LOCUS       DASUYC010000001         4207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_230782,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000001 DASUYC010000000
VERSION     DASUYC010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230782"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VF276_00005"
     CDS             <1..792
                     /locus_tag="VF276_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605272.1"
                     /translation="MKAHHHRGPGPIPTPLRLLTLALTLGLALLLAACGDGGAPPTAA
                     PASGSGSTGGTTLPANPPTATLPTAAQPGGAAPTDTAPGPPPAAAEPTETISPDLPPP
                     DEPPPPTSGPVATVSADEAVVIYSLIAHDLVAQSLTTAATKTTPAYIGISQQAGLGAL
                     LDTDSSNVTIPDDVIDEMGDLGTTVTFSPFMDAIGALEDGAKVRDDGIYLTLGVLEDQ
                     GTPDKIAAYASYYRANDDATGYRYVLEKAAGGPWVIKDRQPMWDH"
     gene            780..2075
                     /locus_tag="VF276_00010"
     CDS             780..2075
                     /locus_tag="VF276_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605273.1"
                     /translation="MGSLTPPDNRRVWAFRAALIGAVVLNLIALGLLVNRPMETLKPP
                     ATPTAGPPQIGPAGTPVPGATPIAGATATGLPRVEHPGGKMRGVNIPVWGPDFVVAKP
                     SIQGAAALDANWVALVTHWYVSSAVPYSGGGIFQEDTGPQRSASDASLGAAIDNAHAL
                     GLKVMLKPHVDWTQGGWRGSFYFDDAKAPGGRQDWWTSYHALISDTVRLAIDHHAEAI
                     CIGTEFADINKEGASAPEWIHIIEQIRAAGFTGQLTYAANWGYGADAEYNRPGLGGVL
                     EQLDFIGIDAYYPLSMERDPTLAQLVAGWHDAINPYDTRPEPMHQDNFAEIEKLHKRT
                     GKPIVFTEVGYPADDFAAKNPAADGGSGNNEALQNRAAEALYQVWGDVPWWQGALWWQ
                     YGPVYNSHSLQGRPILDTLRQVWSEAAETGSPAATAGSP"
     gene            2334..4022
                     /locus_tag="VF276_00015"
     CDS             2334..4022
                     /locus_tag="VF276_00015"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX6605274.1"
                     /translation="MLRHDPGAPPTTPLAGTDNPALAMMRLLLAASALLIIWISPTQP
                     DRLVIPTYLTLGLYTLYSAVLGILTLRRDHLPAVVTQHVHWLDVAWYVVLISLSSGTS
                     SIFFFGFFFAILTASFQCGFAPGLRVTVASAVLYGVVGFATADHAREFELNRALLRPV
                     YLLVLGYLIAYWGGHEITAKRQLAFLKEVSTLSNPRFGVDRTIDLLLRRLQGFYGADA
                     ALLVMCDVGAGVHRLYQTAQGAAAAAGAAPIPAEVANMLLELPAAQPILYPGHALPWQ
                     RFTQRARRRLRRSGAGAAAPRHAELGAAVAALLEVDSFISVSLRNSGGTTGRIYLTAR
                     RRFADSDMHFLTQVAEHVSPVLENIRLVDRLAADAAEHERERIARNIHDSVIQPYIGM
                     QMGLAAVRDKLAAGRGDVTADLDRLIALAGTGITDLRGFARKLPGEVAPEGSLLPAMR
                     RFTDKFTDATGIAVALDVPADLYLHDRLAAEVFQIIAEGLSNVRRHTQAAHAAIHLQC
                     RADQLHLCIENDGVDNAPAFMPRSITGRATALGGHVSVGHPTPDHTAVLVEIPL"
BASE COUNT          687 a         1534 c         1341 g          645 t
ORIGIN      
        1 atgaaagcgc accaccatcg cggccccggt cccatcccga ccccgctccg gctcctgacg
       61 ttggccctga cgctgggcct cgccctgctg ctggccgcct gcggggatgg cggggcgccg
      121 cccacggcgg ccccggccag cggcagcggc agcaccggcg gcaccaccct accggccaac
      181 ccgccgacgg ccacgctccc cactgccgcc cagcccggcg gcgcggcccc gaccgacacg
      241 gcgcccggcc cgccccccgc cgccgcggag ccgaccgaga ccatctcgcc cgacctcccg
      301 ccgccggacg agccgccccc gcccacgtcc ggccccgtcg ccaccgtcag cgccgacgaa
      361 gccgtggtca tctacagtct catcgcccat gacctggtgg cccagagcct aaccacggcg
      421 gccaccaaga ccacgcccgc gtacatcggc atcagccagc aggccgggct gggcgccctg
      481 ctcgacaccg actcctcgaa cgtcacgatc ccggacgacg tgatcgacga gatgggcgac
      541 ctgggcacca ccgtcacctt cagcccgttc atggacgcca tcggcgcgct ggaggacggc
      601 gccaaagtgc gcgatgacgg catctacctc acgctcggcg tgctcgaaga ccagggcacc
      661 cccgataaga tcgccgccta cgccagctat taccgcgcca acgacgatgc caccggctat
      721 cgctatgtgc tcgaaaaagc cgccggcggc ccgtgggtca tcaaggatcg gcaaccgatg
      781 tgggatcact aacgccgccc gacaaccggc gcgtctgggc ctttcgcgcg gcgctgatcg
      841 gcgccgtcgt cttgaacctg atcgcgctgg gtttgctggt gaatcgcccg atggagacac
      901 tcaagccacc cgccactccg accgccgggc cgccgcagat cggccccgcc ggcacgcccg
      961 tgcccggcgc cacgcccata gccggcgcca cggccaccgg cctgccccgc gtcgagcacc
     1021 ccggcggcaa gatgcgcggc gtcaatatcc ccgtctgggg gccggacttc gtcgtcgcca
     1081 agccgtcgat ccagggcgcc gccgcgctcg acgccaactg ggtggccctg gtcacgcact
     1141 ggtatgtgtc gtccgccgtg ccctacagcg gcggcggtat cttccaggag gacacgggcc
     1201 cccagcggtc ggccagcgat gccagcctgg gcgcggccat cgacaacgcg cacgccctgg
     1261 gcctcaaggt gatgctcaag ccgcacgtgg actggaccca gggcggctgg cgcggctcgt
     1321 tttacttcga cgacgccaag gcgcctggcg ggcggcagga ctggtggacc agctaccacg
     1381 cgctgatcag cgatacggtg cgcctggcca tcgaccacca cgccgaggcc atctgcatcg
     1441 gcaccgagtt tgcagacatc aacaaggagg gcgccagcgc tccggagtgg atccacatca
     1501 tcgagcagat ccgcgcggcg ggcttcaccg ggcagctcac ctacgcggcc aactggggct
     1561 acggcgccga cgccgaatac aaccggccgg ggctgggcgg tgtgctggag caactcgact
     1621 tcatcggcat cgacgcctac tatccgctgt cgatggaacg cgaccccacc ctggcccaac
     1681 tcgtcgccgg atggcacgac gcaatcaacc cctacgacac ccgccccgag ccgatgcacc
     1741 aggataattt tgcggagatc gaaaagctgc acaagcgcac cggcaagccg attgtcttca
     1801 ccgaggtcgg ctatccggca gacgacttcg ccgcgaagaa cccggcggcg gacggcggct
     1861 ccggcaacaa cgaggcgctg caaaatcgcg ccgcggaggc gctctaccag gtctggggcg
     1921 acgtaccctg gtggcagggc gcgctctggt ggcaatacgg cccggtctac aactcgcaca
     1981 gcctccaggg ccgccccatc ctcgacacct tgcgccaggt ctggtcggaa gccgccgaaa
     2041 ccggcagccc cgcggccacg gcgggcagcc cgtagcaaaa gagcggcttg tgagcgcata
     2101 gaacatccga tataatcagc ataacttgga acaggcttgt acaggggcgc gtagaggtag
     2161 attggcagcc agcacggggg atggcacaag cgtttaagcc ggtgactcgc cattgagggt
     2221 agggatcgtt tgcgggcggc cgccggtagg cggggcggtt gccatacccg ccggctttca
     2281 gcttgccacc cttcgcctgt atatacgact cgccgattac ccgagccatc gctatgctcc
     2341 gccacgatcc gggcgcgccc cccacgaccc cgctagccgg caccgataat cccgcgctgg
     2401 cgatgatgcg gctgctgctg gctgcatcgg ccttgctgat tatctggatc tcgcccaccc
     2461 aacccgaccg cctggtcatc cccacctatc tgacgctggg tctgtatacc ctctatagcg
     2521 cggtgctggg catccttacc ctgcgccggg atcatctgcc cgccgtggtt acccagcacg
     2581 tgcattggct ggatgtcgcc tggtatgtgg tgctgatctc gcttagctca ggcaccagca
     2641 gcatcttctt tttcgggttt ttctttgcca tcctgaccgc gtcgttccag tgcgggttcg
     2701 cccccggtct gcgggtgacg gtagcctcgg cggtgcttta cggggtagtc ggcttcgcca
     2761 ccgcggacca tgcgcgggag tttgagttga accgggcgct actgcggccg gtatacctgc
     2821 tcgtgctcgg ctatttgatc gcgtactggg gcggccacga gatcaccgcg aaacgccaac
     2881 tggcgttctt gaaggaagtc tcgaccctct ccaacccgcg cttcggcgtg gaccgcacca
     2941 tcgatctgct gctgcggcgc ttgcagggtt tctatggcgc cgatgccgcg ttgctggtca
     3001 tgtgcgacgt gggggccggg gtgcatcgcc tgtaccagac cgcccagggt gccgccgcgg
     3061 ccgccggggc ggcgccgatt cccgccgagg tggccaatat gctgctggag ctaccggccg
     3121 cacagccgat tctctaccca ggtcatgccc tgccctggca gcggttcacg caacgcgccc
     3181 ggcgccggct ccgccggtca ggggccggcg cggcagcccc gcggcacgcg gaactgggcg
     3241 ctgccgtggc ggcgctgctg gaagtggatt cgttcatcag cgtatcgctc cgcaacagcg
     3301 gcggcacgac cggccggatc tatctcacag cccgccggcg cttcgccgac tcggatatgc
     3361 acttcctgac ccaggtcgcc gaacacgtct cgccggtcct cgaaaacatc cggttggtcg
     3421 atcggctggc cgccgatgcg gcggagcacg agcgggagcg catcgcccgg aatatacacg
     3481 acagcgtcat ccaaccctac attgggatgc aaatgggcct ggcggcagtc cgggacaaac
     3541 tggccgcggg ccggggcgac gtgacggccg atctcgatcg gctgatcgcg cttgccggca
     3601 ccgggattac ggatctgcgg ggattcgcac gcaagttgcc cggcgaggtg gcgccagagg
     3661 gcagcttgct gcccgcgatg cggcggttca cggacaagtt taccgatgcc acgggaatcg
     3721 ccgtggcgct cgacgtgccg gcggatctct atctccacga ccgcctggcc gctgaggtgt
     3781 tccagatcat cgccgagggg ttgagcaacg tccggcgcca cacccaggcc gcccatgccg
     3841 cgatccacct gcaatgccgg gccgaccagt tgcacctctg tatcgagaac gacggcgtag
     3901 acaacgcgcc cgcgtttatg ccgcgctcga tcaccgggcg ggccacggcg ctgggcggcc
     3961 acgtcagcgt cggccatccc acgccggacc acacggcagt gctggtcgaa atcccccttt
     4021 aggctactgt gttggagatc gagcatggac agtgcgaccc cccggcccat ccgcattttg
     4081 ttggtcgaag accacggcat cgtgcgcgcc ggcatccgga tgcttatcga gaactcaccc
     4141 ggccagaccg tggtcggcga ggcagccggc cgggaccccg cgctggacct ggccgcgcac
     4201 acccaac
//
LOCUS       DASUYC010000002         2431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_692634,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000002 DASUYC010000000
VERSION     DASUYC010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692634"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="VF276_00020"
     CDS             complement(<1..799)
                     /locus_tag="VF276_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010995697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HEX6605275.1"
                     /translation="MSETKSIQDVDSPRTRATLAADLRALGLEPGMTVLVHSSLSAIG
                     YVVGAAVAVVQALMDVVTPEGTLVMPTHTSENSEPSYWRHPPVPEAWWPIIRDTMPAF
                     EPAITPTRQMGRIVETFRTWPGVRRSAHPALSFAAWGRHAAFVTADHALEYSLGEHSP
                     LARVYDLDGWVLLLGVGHGNNTSLHLGEFRAGTSGDELQGAALLEDGRRVWKTYRDTA
                     WDDDPFPEIGAAFDATGQTRIGKIGSAESRLFRQRAAVDFAQQWLAAR"
     gene            1058..2095
                     /locus_tag="VF276_00025"
     CDS             1058..2095
                     /locus_tag="VF276_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6605276.1"
                     /translation="MAIIEVDDLRKTFRSKRKEAGLRGSLKALVRPDYHVVEAVKGIS
                     LRCERGELLAFIGPNGAGKSTTIKMLTGILYPSAGRAQVLGLTPWRDRERLAFNIASV
                     FGQKSQLWIHLPPLDTFNLLARIYELEDRAYRARRDYLIEVFDIGDLVNTPARRLSLG
                     ERMRCEFAAALIHRPQVLFLDEPTIGLDVVTKQRIRDLIRQLNREEQVTVFLTSHDAG
                     DIEQLCKRVIVINHGTMILNTSVSTLRRRYLKHKIVDLKLQDPLPPEGLQLPGATVLK
                     TSTYGAKLQVDMAVTGIDAVIGRLVAAYQVADITIEDPPLEEIIAAIYAQPAESAAPA
                     GATPVAVQVSP"
     gene            2092..>2431
                     /locus_tag="VF276_00030"
     CDS             2092..>2431
                     /locus_tag="VF276_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605277.1"
                     /translation="MSSIASNPQAELGALGVAGLRGPGPWPARLGKYAAVLRMSIANN
                     IAYAGEVILRSVFLVVLVYVFLQLWHVTYGVLNQPTIGGYNIGQMIWYFAFAEAIILS
                     APRLAQRIDQE"
BASE COUNT          404 a          839 c          810 g          378 t
ORIGIN      
        1 cgcgcgccgc cagccactgt tgcgcgaagt ccacagcggc gcgctggcgg aacagccgcg
       61 actccgccga gccgatcttg ccgatccgcg tctgcccggt ggcgtcgaac gccgcgccga
      121 tttcggggaa cggatcgtcg tcccaggcgg tgtcgcggta ggtcttccag acgcggcgcc
      181 cgtcttccag cagggcggcg ccctgtagct cgtcgcccga cgtgcccgca cgaaactcgc
      241 ccaggtgcag cgaggtgttg ttgccgtggc ccacgcccag caacagcacc cacccgtcga
      301 ggtcgtagac gcgggccagc ggcgagtgct cgcccagcga atactccagc gcatgatccg
      361 cggtgacgaa ggcggcatgg cggccccagg cggcaaacga gagcgccggg tgggcgctgc
      421 gccgcacgcc cggccaggtg cggaacgtct cgacgatgcg gcccatctgc cgggtcgggg
      481 tgatggccgg ctcaaaggca ggcatcgtgt cgcggatgat cggccaccag gcttcgggca
      541 ctggcgggtg ccgccagtag gacggctcgg agttctcaga ggtgtgcgtg ggcatgacca
      601 gcgtgccttc cggcgtcacc acgtccatca gcgcctgcac cacggccacc gccgcaccca
      661 cgacatagcc gatggcgctc aacgacgagt ggaccagcac ggtcatgccc ggttccagcc
      721 cgagcgcgcg taaatcggcg gccagcgtgg cgcgggtgcg cgggctgtct acatcctgga
      781 tcgatttcgt ttcgctcacg atgcccctcc ggacaattgc cgctgagtgc cgtctggtca
      841 acgcaggatc gtttgtccgc aggcagtata ccatcccggc cgcggtgccg tcatcgccgc
      901 ctggaccgag ttcccgccgc cgccgttagt cgtacgcctc atgagcgaaa gcggctacac
      961 ccccgccgat tgcggcacgc gaaatgcgcg tgctacaatt acccacaatg gatccgtagc
     1021 cgtgcatatg cggcggattc gggcggggag gtgcgctatg gcaatcatcg aggtagacga
     1081 cctgcggaag acctttcgca gcaaacgcaa ggaggcgggg ctgcgcggca gcctgaaggc
     1141 gctggtgcgg cccgactatc acgtggtcga ggcagtcaag ggcatcagcc tgcgctgcga
     1201 gcggggcgag ttgctggcct tcatcgggcc gaacggcgcg ggcaagtcga ccactatcaa
     1261 gatgctgacc ggcatcctct atcccagcgc tggccgcgcc caggtgctcg gcctgacccc
     1321 ctggcgcgac cgcgagcggc tggccttcaa catcgcctcg gtcttcggcc agaagtcgca
     1381 gctctggatc cacctgccgc cgctggatac cttcaacctg ctcgcgcgca tctacgagct
     1441 ggaggaccgc gcctatcgcg cccggcgcga ctacctgatc gaggtcttcg acatcggcga
     1501 cctggtgaat acgcccgcgc gccggctcag cctgggcgaa cgcatgcgct gcgagttcgc
     1561 cgccgcgctg atccaccggc cccaggtgct gttcctggac gagccgacca tcggcctgga
     1621 tgtcgtcacc aagcagcgca tccgcgatct gatccggcaa ctgaaccgcg aggagcaggt
     1681 gacggtcttc ctgacctcgc acgacgcggg cgacatcgag cagttgtgca agcgggtcat
     1741 tgtcatcaac cacggcacga tgatcctgaa cacgagcgtc agcacgctgc gccgccgcta
     1801 cctcaagcat aagatcgtcg acctcaagct gcaagacccg ctgccgccgg agggactgca
     1861 gttgccgggc gccaccgtgc tgaagaccag cacttacggc gcgaagctgc aagtcgatat
     1921 ggcggtcacg ggcatcgacg cggtgatcgg gcggctggtg gccgcgtacc aggtggccga
     1981 catcaccatc gaggacccgc cgctggagga gatcatcgcc gcgatttacg cccaaccggc
     2041 ggagagcgcc gcccccgcgg gcgccacgcc ggtggcggtg caggtgtcgc catgagcagc
     2101 attgcgagca atccgcaggc cgaactgggt gcgctagggg tcgccgggct gcgcgggccg
     2161 ggaccgtggc ctgcccggct gggcaagtac gccgccgtgc tgcgcatgag catcgccaac
     2221 aacatcgcct acgcgggcga ggtgatcctg cgcagcgtct tcctggtcgt gctggtctat
     2281 gttttcctgc aactctggca cgtcacctac ggcgtgctca accagccgac catcggcggc
     2341 tacaacatcg gccagatgat ctggtacttc gccttcgccg aggcgatcat cctgtccgcg
     2401 ccgcggctgg cccagcgcat cgaccaggag g
//
LOCUS       DASUYC010000003         3026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_692237,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000003 DASUYC010000000
VERSION     DASUYC010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692237"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VF276_00035"
     CDS             complement(<1..216)
                     /locus_tag="VF276_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605278.1"
                     /translation="MGANTGWFRRAAGRSQTGPYSAIRVGAGRFATGPYSAIPVAGRF
                     ETGPYNAICWRRAGRKPAPTAPFYIRCI"
     gene            complement(206..1852)
                     /locus_tag="VF276_00040"
     CDS             complement(206..1852)
                     /locus_tag="VF276_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605279.1"
                     /translation="MRHVRSLFSLGLVALLFGIWPAAAPRAFAQSAGCKSFPQTGHTL
                     CGQFLAYWESRGGLAQEGYPISDEITAVNPLDGKTYTQQIFERAVMEKHPENAPPYDT
                     LLMQLGTLRYKQKYPQGAPGQTPAAGGQWFGQTGHRIGGQFLAYWQQHGGLAQQGYPI
                     SDEFTEVSELDGKPYKVQYFERAVFEYHPENAPPNDVLLSQLGTFHARSLPPGTLQAA
                     APPVQVAAKAPFQKGLNFVSWYAGGYASAAADQALAQLAATGANTLAVVVTGYQDTIG
                     STSIRLDSPRTPTDADLAHVIATAHRLGLRVLLKPHVDLANDPGHWRGQIGQAFSGEG
                     QWQAWFAAYRDFIGHYAALAQQNGADLLCVGTELVGTSAREADWRNVISTVRSKYQGQ
                     LTYAANWGDDVVSIRWWDALDYIGVDAYYPLTGKTGPTADEIARAWVDRGYVGTLEGL
                     AGRFGKQIVFTEIGYGSVAGTNRAPYDWQAGGTLDLQEQANAYQAAFAVFWGKPWLAG
                     MYWWSWDADPNTGGAGDKTYTPHNKPAEQVLKSFYSGGGR"
     gene            complement(2154..>3026)
                     /locus_tag="VF276_00045"
     CDS             complement(2154..>3026)
                     /locus_tag="VF276_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX6605280.1"
                     /translation="FRPAEPHPDRFRSLTGLWDRTPLRPADLAPAEDFHWRSVDGLEI
                     QGWLYRAPAPARGTVVYIHGGPTAHSMDAVNTQIQFFVNGGFNVLDPNYRGSTGFGLR
                     FRDAIKEDGWGGREQDDIRTGIEALIAAGIAAPGKVGVTGTSYGGYSSWCAITHCPPE
                     IVAASAPICGMTDLVVDYETTRPDLRPYSEEMLGGRPDQVPERYHERSPIHFVQNIRG
                     RLLVVQGMQDPNVTPENVRSVRAALDEAGIPYEILAFEDEGHGILRPRNQKTLFLRLA
                     EFFGQAFADPAPAR"
BASE COUNT          449 a          987 c         1097 g          493 t
ORIGIN      
        1 tatgcaacga atatagaaag gcgctgtagg ggccggtttg cgaccggccc gccgccagca
       61 gatcgcgttg taggggccgg tttcaaaccg gcccgccacc gggatcgcgc tgtaggggcc
      121 ggttgcaaac cggcccgccc ccacccggat cgcgctgtag gggccggttt gcgaccggcc
      181 cgccgcgcgg cggaaccagc cggtgttagc gcccaccccc gctgtagaac gatttcagca
      241 cctgctcggc gggcttgttg tgcggggtat acgttttgtc gcccgcgccg ccggtgttcg
      301 ggtcggcatc ccagctccac cagtacatgc cggccagcca gggcttgccc cagaagacgg
      361 cgaaggccgc ctggtaagcg ttggcctgct cttgcaggtc gagcgtgccg ccggcctgcc
      421 agtcgtaggg cgcgcgattg gtgcccgcca cgctgccgta gccgatctcg gtgaagacga
      481 tctgcttgcc gaaccgcccg gccagcccct ccagcgtgcc cacatagccg cgatcgaccc
      541 aggcgcgggc gatctcgtcc gccgtcgggc cggtcttgcc ggtcagcggg tagtaggcgt
      601 ccacgccgat atagtccagg gcgtcccacc agcggatgct gaccacgtcg tcgccccagt
      661 tggcggcata ggtcagttgg ccctgatact tgctgcgcac cgtgctgata acattgcgcc
      721 aatcggcctc acgggcgctg gtgccgacga gttcggtgcc gacgcagagc agatccgcgc
      781 cgttttgctg ggccagggcg gcgtagtggc cgatgaagtc gcggtaggcg gcgaaccagg
      841 cttgccactg gccctccccg ctgaacgcct gcccgatctg gccgcgccag tggccgggat
      901 cgttcgccag atcgacgtgc ggcttgagca gcacccgcaa gcccagccgg tgagcagtgg
      961 cgatgacgtg ggccaggtcg gcgtcggtgg gcgtgcgcgg cgagtccagg cggatgctgg
     1021 tcgagccgat cgtgtcctgg tagccggtca cgaccacggc cagggtgttg gcgccggtgg
     1081 ccgccagttg ggccagcgcc tggtcggcgg cggcggacgc atacccgccc gcgtaccagg
     1141 agacgaagtt gaggcccttc tggaacggcg ccttggcggc gacctgcacg ggcggcgcgg
     1201 cggcctgcag ggttcccggc ggcaggctgc gcgcgtggaa ggtgcccaac tggctcaaca
     1261 gcacgtcgtt gggcggggcg ttctccgggt ggtactcgaa cacggcgcgc tcgaagtatt
     1321 gcaccttgta gggcttgccg tcgagttccg acacctcggt gaactcgtcg ctgatggggt
     1381 agccctgctg ggccagcccg ccgtgctgct gccagtaggc caggaactgg ccgccgatgc
     1441 ggtggccggt ctgcccgaac cactgcccgc ccgccgcggg ggtctggccc ggcgcgccct
     1501 gcgggtactt ttgcttgtag cgcagcgtgc ccagttgcat caagagcgta tcgtagggcg
     1561 gcgcgttctc ggggtgcttc tccatcaccg cgcgctcgaa gatctgctgc gtataggtct
     1621 tgccgtcgag cggattcacc gccgtgatct cgtcgctgat cgggtagcct tcctgagcca
     1681 gcccgccgcg gctttcccag taggccagga actggccgca gagggtatgc ccggtctggg
     1741 ggaaggactt gcatccggcg ctttgcgcga acgcccgcgg ggccgccgcg ggccatatgc
     1801 cgaagaggag ggctaccagc cccagggaga acagagagcg cacatgtcgc atggagtgga
     1861 tcggcctttc gtgcatagat ggaggatggc aacctgacta tgatactgag gggtccgcaa
     1921 ggccggcagg ctcggccagg atactgcatt ccggcgcggg aaccgggcga ccacaaccgc
     1981 agcggtcacg aaatcattat gcccgatatg cggtcacttt cgcgtcaatt ttccgcgcga
     2041 atcgtcacag tcggcacaca atgtcctagc gaccgcagcg ccggggcgcc ggcagcaaaa
     2101 cgcccgtggc cggggcggtc acgggcgtta cgtctgagca gaggcgcggg actctagcgc
     2161 gcgggcgccg gatccgcgaa ggcctggccg aagaactcgg cgaggcgcag gaagagcgtc
     2221 ttctggttgc ggggccgcag gatgccgtgg ccctcgtcct cgaaggccag gatctcatag
     2281 gggatgcccg cctcgtcgag ggctgcgcgc accgagcgca cgttctccgg cgtgacgttg
     2341 gggtcttgca taccctggac gacgagcagc cgcccgcgga tgttctgcac gaagtggatc
     2401 ggcgagcgct cgtggtagcg ttcgggcacc tggtcgggcc ggccacccag catctcttcg
     2461 ctgtagggcc gcaggtcggg gcgcgtggtc tcgtaatcga ccaccaggtc ggtcatgccg
     2521 cagatgggcg ccgaggcggc gacgatctcc ggcgggcaat gggtgatggc gcaccacgac
     2581 gagtagccgc cgtacgacgt gccggtcacg cccactttgc ccggtgcggc gatacccgcc
     2641 gcgatcagcg cctcgatccc ggtgcggatg tcgtcctgct cgcgcccgcc ccagccgtcc
     2701 tccttgatcg cgtcgcggaa gcgcaggccg aagccggtgc tgccccggta attggggtcc
     2761 agcacgttga agcccccatt gacaaagaac tggatctggg tgttgacggc gtccatgctg
     2821 tgcgcggtcg gtccgccatg tatgtagacg acggtgccgc gcgcgggggc gggcgcccga
     2881 tagagccagc cctggatctc caggccatcg accgagcgcc agtggaagtc ctcggcgggc
     2941 gcgagatcgg cggggcgcag gggcgtgcgg tcccagaggc cggtcaggct gcggaagcgg
     3001 tcggggtggg gctcggcggg gcggaa
//
LOCUS       DASUYC010000004         2513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1381517, whole genome shotgun sequence.
ACCESSION   DASUYC010000004 DASUYC010000000
VERSION     DASUYC010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381517"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..1343
                     /locus_tag="VF276_00050"
     CDS             54..1343
                     /locus_tag="VF276_00050"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6605281.1"
                     /translation="MAVATPAPAATPARTRQPRVVIIGAGFGGLNAVRTLAGTPARVL
                     VLDRNNYHGFWPLLYQVATAGLEPESIAYPVRAIVRRYRNAGFRMGQVDGIDFARRLV
                     RASDRQIPYDYLILAAGSANNYFGNDALAEHTYGLKDIGDGERLRNRILSSFEAAVSE
                     PDPARRQALLTFVIVGAGPTGVELAGAIAELIHHVLRKDYPMLDVGRARVVLVEAIDR
                     VLAAFPESLQRSARRRLEQLGVEVILNAPVKSVSEQAVTFADGQPLPARTVVWAAGIR
                     SAHLVDALDLPLGRGARVKVLPTLNVPGHPEVFAIGDLVYLEGYKGDQPYPQVAPVAI
                     QQGRHAARNILAQMAGRPMRPFHYFDKGSMATIGRRAAVFDAFGIRLSGVLAWFGWLF
                     VHLIYLIGFRNRLIVLANWIYNYFTYDRGVRLISGDE"
     gene            1543..>2513
                     /locus_tag="VF276_00055"
     CDS             1543..>2513
                     /locus_tag="VF276_00055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX6605282.1"
                     /translation="MTGSSAREERPGTPVQDGTDLPTDADLLQTIERAADEARRQDNA
                     DLLQLITQLQQAFAQRRQADDSLRESEQRYRDMFEKNQAIKLLIDPMTMLIVDANPAA
                     CEFYGYPRDVLTRMTLHEINMLSQEDLAGQLGRAMIEQHSYFYFRHRLASGAVRDVEI
                     HSGPVPIAGRQLLYSIVHDITERKQAEAQVRRQLAFTNAITGSLGEGVYALDREARLT
                     FMNPAAERMLRWTTTELLGRPTHELIHFQRADGTPLPAADCPLLQGIRAGRVVRVDDD
                     IYTRKDGTTFPVAYTSAPIVLDATVVGTVVAFRDMTGPKRAEETLRE"
BASE COUNT          423 a          847 c          818 g          425 t
ORIGIN      
        1 gccggtattc ggcagcacgc gcaatcatgg gcaagcgcaa agcggagaca cctatggcag
       61 tggcaacacc ggcacccgcg gcaactcccg cccggacgcg gcagccgcgg gtggtgatta
      121 tcggcgcggg gttcggcggc ctcaacgccg tgcgcaccct ggcgggcacc ccggcgcggg
      181 tgctggtact cgaccgtaac aactatcacg gcttctggcc gctgctctac caggtggcga
      241 cggcgggcct cgaaccggag tccatcgcct acccggtgcg cgccatcgtg cgccgctacc
      301 gcaacgccgg cttccgcatg ggccaggtcg acggcatcga cttcgcccgc cgcctggtgc
      361 gcgccagcga ccggcagatt ccctacgact acctgatcct ggcggcgggc agcgccaata
      421 actacttcgg caacgacgcc ctggccgagc atacctacgg cttgaaggac atcggcgacg
      481 gtgagcgcct gcgcaatcgc atcctctcgt cattcgaggc cgcagtgagc gagcccgacc
      541 cggcccgccg ccaggcattg ctcaccttcg tgatcgtcgg ggccgggccg acgggggtgg
      601 aactggcggg cgccatcgcc gaactgatcc accacgtact gcgcaaggat tacccgatgc
      661 tcgacgtggg cagggcccgc gtggtcctgg tcgaggccat cgaccgcgtg ctggcggctt
      721 ttccggagtc gttgcagcgc agcgcccggc gccgcctgga acaactgggc gtggaagtta
      781 tcctcaacgc gccggtcaag tccgtgagcg agcaggcggt gacatttgcc gatgggcagc
      841 cgctgccggc gcgcaccgtt gtctgggcgg cgggcatccg cagcgcgcac ctggtcgatg
      901 cgctggatct gccgctgggg cgcggggcgc gggttaaagt gctgcccacg ctcaatgtgc
      961 ccggccaccc ggaggtgttt gcgatcggcg acctggtcta cctggagggc tacaagggcg
     1021 accagcccta tccgcaggtg gcgccggtgg cgatccagca gggccggcac gcggcgcgca
     1081 atatcctggc gcagatggcg ggccggccca tgcggccttt ccactacttc gacaaaggca
     1141 gcatggcgac tatcgggcgg cgggccgccg tgttcgatgc tttcggcatc cggctcagcg
     1201 gggtgctggc ctggttcggc tggctgttcg tccacctgat ttatctgatc ggcttccgca
     1261 accgcctgat cgtgctggcg aactggatct ataactactt cacctatgat cgtggggtgc
     1321 gcctgatttc gggagatgag taaacgaatc ggtcaaaata tggtattctt aaggaagtgc
     1381 cgggacttgt cccgcgggat cactcctggg tccgggtcta cgccccgcct ggtgcgggca
     1441 tgatcttctc ctctctggca tgagctgggt tcggcgcgtc catggcaggt attcgcccct
     1501 gggttccgtc cccatcgcca acgtagaaaa cgaggcacct tgatgactgg gtcatccgcg
     1561 cgtgaagagc gccctggcac tccggtccag gatgggactg acttaccgac cgacgccgat
     1621 ctcctgcaga ccatcgaaag ggcggcggac gaggcgcgcc ggcaagacaa cgccgatctg
     1681 ttgcaactga ttacgcaact ccagcaggcc tttgcccagc gccgccaggc cgatgactcg
     1741 ttgcgcgaaa gcgaacagcg ctatcgcgac atgttcgaga aaaaccaggc catcaagctg
     1801 ctcatcgatc cgatgaccat gctgattgtc gacgccaacc ccgccgcctg cgagttctac
     1861 ggctatccgc gcgacgtgct gacccgcatg accctgcatg agatcaacat gctctcgcag
     1921 gaggacctgg ccgggcagtt gggccgggcc atgatcgagc agcacagtta cttttacttc
     1981 cgccaccggc tagcctccgg cgccgtgcgc gacgtggaga tccactccgg gccggtgccc
     2041 atcgccgggc ggcaactgct ctactcgatt gtccacgata ttaccgagcg caagcaggcc
     2101 gaagcgcagg tgcgccgcca gcttgctttc accaatgcga ttaccggtag cctgggcgaa
     2161 ggggtctacg cgctggaccg cgaggcccgg ctcaccttca tgaacccggc cgcggagcgc
     2221 atgctgcgct ggaccaccac ggaactgctg ggccgtccga cgcacgaact tatccatttc
     2281 cagcgcgccg acggaacgcc gctgccggcg gccgactgcc cgctgctgca gggcatccgg
     2341 gccggacgcg tcgtccgcgt ggacgacgac atctatacgc gtaaagacgg caccaccttc
     2401 cccgtggcct acacctcagc gccgattgtc ctcgacgcta ccgtggtcgg cacggtggtg
     2461 gccttccgcg atatgaccgg gccgaagcgc gccgaggaga ccctgcgcga gag
//
LOCUS       DASUYC010000005         4059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1381352, whole genome shotgun sequence.
ACCESSION   DASUYC010000005 DASUYC010000000
VERSION     DASUYC010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381352"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="VF276_00060"
     CDS             complement(<1..580)
                     /locus_tag="VF276_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605283.1"
                     /translation="MTSRPLSVRVASVSVLALVVFALGFAFGPHLASAGPAAAATLST
                     LTIQGSITSADRTQAGRIIRDWAPSACGAVKSAPDTGDTAAHHYDAYNYTNTSASNQC
                     VTVQLRSACTGSNDVFSIAYLGSFNPASIKTNYRADLGLSPGQDSGTTGYSFDVPAGQ
                     SFAVTVYEVTANAGCGGYTLDVTGAGITGGSTG"
     gene            1167..2390
                     /locus_tag="VF276_00065"
     CDS             1167..2390
                     /locus_tag="VF276_00065"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX6605284.1"
                     /translation="MPEQTTTRIRTLGVLTGGGDVPGLNAAIKALVYRAETMGIRVTG
                     IRAGWEGITYLDRARGRDDLIFRPFDRTTWHGSYLMPLDRLNTRTIDRQGGTILQSSR
                     TNPARTKVSELPPHLRSYGEGHSPDDRVDLTGEVLKNLEFLDLDGLVVIGGDDTLSYG
                     SVLTRRGVPVWGIPKTMDNDVPGTDYCIGFQTAISRAGEFIGRVRSTAGSHRETVLFR
                     LFGRDAGFTALETAIVTSADRLLIPEVPTSVDRLAELVAVDRENPSGYSIVVMSEGAN
                     LGGTAVPEVGPADAYGHRKKVNIAEFLAEELGARLPHVRFLPIDLTYFLRSGEPEVYD
                     KRMAIFYANLVMNLVAEGTHGVMAAYRDGEYICTDVPGKNLHARRVDPADYDMQEYRP
                     NFVNIGTTYRPQAEP"
     gene            2592..2753
                     /gene="rpmH"
                     /locus_tag="VF276_00070"
     CDS             2592..2753
                     /gene="rpmH"
                     /locus_tag="VF276_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX6605285.1"
                     /translation="MSKRTYQPKRVPRKREHGFLKRMATRGGRLVLAARRQKGRYRLT
                     IGSEQKGPR"
     gene            3150..3359
                     /gene="yidD"
                     /locus_tag="VF276_00075"
     CDS             3150..3359
                     /gene="yidD"
                     /locus_tag="VF276_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX6605286.1"
                     /translation="MKTLALALIRLYQKTISQALPPTCRFVPSCSEYGYQAIAKHGFL
                     KGSLLAGWRILRCNPFNDGGFDPVP"
     gene            3373..>4059
                     /locus_tag="VF276_00080"
     CDS             3373..>4059
                     /locus_tag="VF276_00080"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HEX6605287.1"
                     /translation="MPIWDQFVQLLEESLKLINQWVGNPGVAIIIFTLIVKVLTLPLT
                     LKSIKSMRETQRIQPLIKEVNKRFKDDKAKQQAEVMRLYQQHGVNPMGSCLPMVLQIP
                     IFLALYNALITLVQHDQSFASAFLWVHDLSKADPLFIWPVLSGAFQFIGNRMAQPYGS
                     TKNADPQQAMMNKIMQFMPIYLIVIYLNFAAGAVIYWTFSALFSAVQTYFINGFGTLP
                     EVPGLGWLPKR"
BASE COUNT          764 a         1313 c         1239 g          743 t
ORIGIN      
        1 cgccggtgct gccccccgtg atgccggccc cggtcacgtc gagggtatag ccgccgcagc
       61 ccgcgttggc cgtcacctcg tagacggtga cggcgaagct ctgcccggcg ggcacgtcga
      121 aggagtagcc ggtggtgccg ctatcttgcc ccggactcag gcccaggtcg gcgcgatagt
      181 tggtcttgat actcgccgga ttgaagctgc ccaggtaggc gatgctgaac acgtcgttgc
      241 tgcccgtgca ggccgagcgc agttgcaccg tcacacactg gttgctcgcg ctggtattcg
      301 tgtagttgta ggcatcgtaa tgatgcgcgg cggtatcgcc ggtatcgggc gcgctcttga
      361 cggcgccgca ggcgctgggc gcccagtcgc ggatgatgcg gcctgcctgg gtgcggtcgg
      421 cactggtgat gctgccctgg atggtcaagg tgctcagggt ggcggcggcc gccgggccgg
      481 cggaagccag gtgcggcccg aaagcgaacc cgagggcgaa tacgacgagg gccaacacac
      541 tcaccgaggc cacacgaacc gaaagaggtc gggaagtcaa aaggcattct cctctgtaca
      601 tggatgtggt tccaacatgt tcgcgatcct cgaagccaca cattcgagaa cgcgcccact
      661 tcacatcccc aattgtccca tattgacgac gttcatcgtc accgcaatcc taccaacatc
      721 cgcagttaca tcgcaattac taaaaggccc atccgtatca atttaaggtc acaactccga
      781 tgtggatgat catgcctatc atatggcgtg ccgcgcatga cggaggccgg ttccatgccc
      841 atctgcgcat gaatcatcct gttctgcagg ggatgttctg cttctatccg gagacgatat
      901 atagatccca cacaggaaca tctgctcgta agtagttgcg gatgctccgg cccgccgcag
      961 gctaacctcg ccccccggcc ccttctcgcc gcggatctcc gcacttcttt tctgccagcc
     1021 accgtactca gcacgcagca ttcagtactc gtctgggcga ggattaggtt gcgcccatcg
     1081 ccgcccgcca cgcacgggtc gtattctggg tcaatttgtg gtatgctgtc atgaaaacga
     1141 tctgttcttc tcaggaggac tcaactatgc ctgaacagac aaccacacgg atccgcacat
     1201 tgggcgtcct gaccgggggt ggcgacgtgc cgggcctcaa cgcggcaatc aaagcgctcg
     1261 tctatcgggc ggagacgatg ggcattcgcg tgaccggcat tcgcgccggg tgggaaggga
     1321 tcacctatct cgaccgggca cggggccgcg acgacctgat cttccggccg ttcgaccgca
     1381 ccacctggca cggcagctac ctgatgccgc tggatcggct caacacccgg accatcgacc
     1441 gccagggcgg caccatcctg caatcgtcgc gcaccaaccc cgcccggacc aaggtgtccg
     1501 aactgccgcc ccatctgcgc agctacggcg aaggccacag ccccgacgac cgggtcgacc
     1561 tgaccggcga agtgctcaag aatctggagt tcctcgacct cgacgggtta gtcgtgatcg
     1621 gcggggacga cacgctgagc tacggctcgg tgctgacccg gcgcggtgtg cccgtctggg
     1681 gcatccccaa gacgatggac aacgacgtgc cggggaccga ttactgcatc ggcttccaga
     1741 cggcgatcag ccgggcaggc gagttcatcg ggcgtgtgcg gtccacggcg gggtcgcacc
     1801 gcgagacggt gctcttccgc ctgttcgggc gcgacgccgg gttcaccgcg ctcgaaaccg
     1861 ccatcgtgac cagcgccgat cggttgctca tcccggaagt gccgaccagc gtcgaccggc
     1921 tggccgaact ggtcgccgtg gaccgggaga acccgtccgg ctattcgatc gtcgtcatgt
     1981 cggaaggggc gaacctgggc ggcaccgccg tgcccgaggt gggccccgcc gatgcctacg
     2041 gccatcgcaa gaaggtgaac atcgccgagt ttctggccga ggaactgggg gcgcggctgc
     2101 cccatgtgcg cttcctgcct atcgatctaa cctacttcct gcgcagcggc gaacccgaag
     2161 tctatgacaa gcgcatggcc atcttctatg ccaacctggt catgaacctg gtggccgagg
     2221 gcacgcacgg ggtcatggcg gcctatcgcg acggcgaata catctgcacc gatgtgccgg
     2281 gcaaaaatct gcacgcgcgc cgggtcgatc ccgccgacta cgacatgcag gaatatcgcc
     2341 ccaacttcgt gaatatcggc accacctacc ggccgcaagc cgagccgtaa gacttgccgc
     2401 cccgcgccgc cacggcgtgg ggtatacccc agccggggag aggtgcgcga gccggggttt
     2461 ggttgacact gccccggcga atccgtataa tgggtaggtg cttcgcggcc tggccggggc
     2521 tgcaacatgc ccgagcgagc gcgaaccgta tccgatattt ttgctatcta cacaggagta
     2581 gctaaccgtc aatgagcaag cgcacttatc agcccaagcg cgtgccgcgc aagcgcgaac
     2641 atggatttct caagcgaatg gcgacccgcg gggggcgcct ggtgctggcc gcccggcgcc
     2701 agaaggggcg ctaccggttg accatcggca gcgagcagaa gggtcctcgc taatcacgga
     2761 gccgcgccgg gaacccgatc cgcccgccgg acgggtcccg tctcccccgc gcttccgctt
     2821 ccgccgggac caccgcctga cgcgggggcc ggagatcgag cgagtccgcc gcaccgggcg
     2881 ctcgtggagc cagggtcccc tggtgctgta tgcggccccc ggtgtcccgg ccgcccccac
     2941 acgggcggcc tttatcgcag gaaagaaagt cggcgacgcc gtccggcgca accgggccaa
     3001 acgctggatg cgtgaagcct ttcgcttaca acttccgtat ataaaacaag gctgggatat
     3061 gatctggatc gcccggccca gtatggatca ggccgagttc cgccgggtcc aggtcgcgat
     3121 ggcggaatgt ttgaagcgag cacacctata tgaaaacact agcgctggcg cttatccgcc
     3181 tctatcagaa gaccattagc caggccctgc ccccgacctg ccggtttgtg ccgagctgtt
     3241 cggaatatgg ttaccaggcg attgccaagc acggcttcct gaaaggctcg ttgctggcgg
     3301 ggtggcgcat cttgcgctgt aaccccttta acgacggcgg attcgacccc gtcccgtaag
     3361 gagacccagg aattgccaat ttgggatcaa tttgtccaac tgcttgagga atcgctcaag
     3421 ctgatcaacc aatgggtggg caaccccggc gtcgcgatca ttattttcac cctgatcgtg
     3481 aaagtgctca ccctgccgtt gaccctgaag tcgatcaagt cgatgcgcga gacgcagcgc
     3541 atccagccct tgatcaagga agtcaacaag cgcttcaagg acgataaagc caagcagcaa
     3601 gccgaggtga tgcgcctgta ccagcaacac ggcgtcaatc ccatgggcag ttgtctgccg
     3661 atggtgctgc agatcccgat cttccttgcc ctctacaacg cactgatcac gctggtgcaa
     3721 cacgaccaga gtttcgcctc ggccttcctg tgggtgcatg atctgtcgaa ggccgacccg
     3781 ctgttcatct ggcccgtgct gtcgggcgcg ttccagttca tcggcaaccg catggcccag
     3841 ccctatgggt cgaccaagaa cgccgacccg caacaggcga tgatgaacaa gattatgcag
     3901 tttatgccga tctatctgat cgtcatctat ctgaacttcg cagcgggcgc cgtcatttac
     3961 tggaccttct cggcgctctt tagcgcggta cagacgtact tcatcaacgg cttcggcacc
     4021 ttgcccgagg tgcccggtct gggctggttg cccaagcgc
//
LOCUS       DASUYC010000006         3044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_689769,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000006 DASUYC010000000
VERSION     DASUYC010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689769"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            253..2964
                     /locus_tag="VF276_00085"
     CDS             253..2964
                     /locus_tag="VF276_00085"
                     /inference="COORDINATES: protein motif:HMM:NF012693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX6605288.1"
                     /translation="MAPPEEAAVTAPPAAGAWDWSAPPALETAEDMVEATGIPAAAAT
                     DQYAEYSVGDYPDFAPEPELLGASETFAPVEPGRADQEDVADGATVTGDTAPANDLEL
                     QPEESDNAPIGLWVAGTPESPDAPASPTPASAAVPNPGLPASEFATAASSSMQEEPAA
                     TPGGDLVGEDMLPVESTGSNTDEAGAAEPISATPPTGSGPAAAGTSDVAGAGTVSAAE
                     LQEAEAAVGNPDEVPATANADLAQPTLVVEAPPTLYAEVPPAAPAEPPTLNAPAPAEP
                     PALNTSAAAEPPALNASAPVESADTPFGTPPASPQTGPLTPAEAGPAEAGAGPDDSAL
                     PTTPIEPDIPMAPAPPIPAEGTTAKLEEGDVPEVAPPAPGDPFPALQPGDVVADRYQI
                     AQVLQTGPQRQTYLAVDLKGYERCWACGSRENVQGEQYCTDCGAQLTGRTYRLFETPP
                     EEPIAAVPAPLLENQHAGVAGVFDRFTDPATGRHYLVLEDVGGAPLSAGPGDQPLTDE
                     RILNWLHQAAATLQELHAARVVGCDFTPAVLQVLADDRLVLTDPTACRLAGTAGDGRT
                     PQAEAQADVQRVAAVLEQWYRGVRPDYPPAAPGETNTVAAVLAHGREGGYPTAADFAG
                     ALQDILDAENPPADMQLVSGRASDVGVQRQLNEDSLFALECVAMEAAGNTPTGLYVVA
                     DGMGGHASGEVASSIAIRTIAGLVGKVVDGRVSGESPPMADEALGKLLRDAILEANHR
                     ITQYSQERHNDMGTTVTMALVLGNRVTVANVGDSRTYLWREGKLHQISQDHSLVARLI
                     AAGQLTAEEGRHFDRRNEIYRALGDAHMTADEIDIFHVRLRPLDALVLCSDGLWEMVR
                     DEDIERIMLDAPDLPTAAQHLIDAANRNGGEDNISVIIAQVLTASED"
BASE COUNT          510 a         1126 c         1033 g          375 t
ORIGIN      
        1 cgttgtggca ccgaaaaccg cactgaagcc cgtttctgcc gcgcctgcgg gcaggccctc
       61 accgctccgg cgatcgcggc gaccgcactc gctccggtga tggagccagg cacgccgctc
      121 ggcgcggcag agaaaacagc agcgaccagc ggcgccctat ccgcaaccgc cggcccgcgc
      181 gatatagacc tgtccgcatt ggccggtgag aatgacgggg ccctcgcaag tgacgcggcg
      241 cccgcctcgg agatggcgcc gccggaggaa gccgccgtga cggcgcctcc tgctgccggc
      301 gcctgggatt ggagcgcgcc gccggcacta gagacggcag aggacatggt cgaagcgacc
      361 ggcattcccg cagccgccgc caccgatcaa tacgccgaat acagcgtggg cgattatccc
      421 gacttcgcac cggagcccga attgctcggc gcgtcggaga cctttgcgcc ggtcgaaccg
      481 gggcgcgctg accaggagga cgtggcagat ggcgccacag tcaccggcga tacggcgccc
      541 gcgaacgacc tggaattgca gccggaggaa agcgataacg cgcccatcgg cctctgggtg
      601 gccggcacgc cggaatcccc tgatgccccg gctagcccta ccccggcgag cgccgcggtg
      661 cccaaccccg gcctgccggc aagcgaattc gccacggcag cctcgtcatc tatgcaagag
      721 gagccggcgg ctacgcccgg cggggatcta gtcggagagg atatgctacc agtggaatct
      781 acgggcagca acacggacga agccggcgcg gcggaaccga tcagcgccac cccgcccacc
      841 ggcagcggcc ccgcagccgc cggcacatcg gacgtggcgg gcgccgggac cgtgagcgcg
      901 gcggaactgc aggaggccga ggcggctgtc ggcaatcccg acgaggtgcc ggccaccgcc
      961 aacgccgatc tcgcccagcc gaccctggtg gtcgaagcgc ccccgaccct ctatgccgaa
     1021 gtgccgcccg ccgccccggc ggaaccgcct accctgaacg cgcccgcccc ggcggaaccg
     1081 cctgcgctga atacgtcggc agcggcggaa ccgcctgcgc tgaacgcatc cgcgccggtg
     1141 gagagcgccg acaccccgtt cggcacgccg cccgccagtc cgcagaccgg ccccttaact
     1201 cccgccgagg ccggcccggc ggaggccggc gccgggcctg acgacagcgc gctgcccacc
     1261 acgccgattg aacccgacat ccccatggcg cccgcgccgc cgatccccgc cgagggcacg
     1321 acggccaaac tggaggaagg tgatgtgccg gaggtggccc cgcccgcgcc gggcgacccc
     1381 ttcccggcgc tccagcccgg cgacgtggtg gccgatcgct accagatcgc ccaggtgtta
     1441 cagaccggcc cgcagcgcca gacctacctg gccgtggacc tcaagggcta tgagcgctgc
     1501 tgggcctgcg gctcgcgcga gaacgtgcaa ggcgagcagt attgcaccga ctgcggcgcc
     1561 caactgaccg gccgtaccta ccggcttttt gagacgccgc cggaggagcc tatcgccgcc
     1621 gtgcccgcgc ccttgctcga aaatcagcat gccggcgtgg ccggtgtctt cgaccgcttc
     1681 accgacccgg cgacgggccg ccattacctc gtactggaag atgtgggcgg cgcgccgctc
     1741 agcgccggac cgggcgatca gcccttgacc gacgagcgca tcctgaactg gctgcaccag
     1801 gccgccgcca cgctgcagga actacacgcg gcgcgcgtgg tcggctgcga tttcacgccc
     1861 gccgtgctcc aggtcctggc cgatgatcgc ctcgtcttga ccgatccgac cgcctgccgc
     1921 ctggcgggca ccgcgggcga cgggcgcacc ccgcaggccg aggcgcaggc cgacgtgcag
     1981 cgcgtcgccg ccgtgctgga gcagtggtac cgcggcgtgc ggcccgacta cccccccgcc
     2041 gcccccggcg agacgaacac cgtggctgcg gtgctggccc acgggcgcga gggcggctat
     2101 ccgacggccg ccgacttcgc cggggcgctg caagacatcc tggatgcgga aaacccgccc
     2161 gccgatatgc agttggtgag cggccgggcc agcgatgtgg gcgtgcagcg ccagttgaat
     2221 gaagacagcc tcttcgctct cgaatgcgtg gcgatggaag ccgccggcaa cacgcccacc
     2281 ggcctctatg tcgtggccga cggcatgggc ggccatgcca gcggcgaggt cgccagttcg
     2341 atcgccatcc gcaccatcgc cggactggtc ggcaaggtgg tggacgggcg cgtcagcggc
     2401 gagagtccgc cgatggcgga cgaggcgctc ggcaagctgc tgcgcgacgc gatcctggag
     2461 gccaaccacc gcatcaccca gtacagccag gagcgccaca acgacatggg caccaccgtg
     2521 accatggccc tggtgctcgg caaccgcgtg acggtggcga acgtgggcga cagccgcacc
     2581 tatctctggc gcgaaggtaa gctgcaccag atcagccagg accactcgct ggtggcccgc
     2641 ctgatcgccg ccgggcagtt gaccgccgag gaaggccgcc acttcgaccg gcgcaatgag
     2701 atttaccgcg ccctcggtga cgcccacatg acggccgacg agatcgatat tttccatgtc
     2761 cgcctgcgcc cgctcgacgc gctggtgctc tgctcagacg ggctctggga gatggtgcgc
     2821 gacgaggaca tcgagcgcat catgctcgac gcgcccgacc tgccgacggc ggcccagcac
     2881 ctgatcgatg ccgcgaaccg caacggcggc gaagacaaca tttcggtgat tatcgcgcaa
     2941 gtgctcacag cttcggagga ctgaaccggc gcgccggggc ctggccccga tctgcaccgg
     3001 cagcccgcat acccaaagtg gaggtggaat cctgcgatgc ctga
//
LOCUS       DASUYC010000007         4054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_2075223, whole genome shotgun sequence.
ACCESSION   DASUYC010000007 DASUYC010000000
VERSION     DASUYC010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2075223"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..503)
                     /locus_tag="VF276_00090"
     CDS             complement(39..503)
                     /locus_tag="VF276_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6194 family protein"
                     /protein_id="HEX6605289.1"
                     /translation="MDEAAITQYITATFPGVDLAETQGATFYFYNPPDLEPDHRFPFA
                     TLVTSDAFDPFSNLNRPGAFRLNIGIGKATFRALFGPPPARAGEEAAPGYDFTALDQV
                     LPHPVYGRQYWVCILNPSPATFATQVQPLLAEAYDLAVGKRTRHGRVLSAEY"
     gene            753..1487
                     /locus_tag="VF276_00095"
     CDS             753..1487
                     /locus_tag="VF276_00095"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX6605290.1"
                     /translation="MIVEATLRTIDLARAGRISVQQVRNYEAAGLIPPAARGPNGYRR
                     YTRKHLVALTTARALVGSYGPLHAAALMQAVHRGDLPAALALIDARHAELAATRQQVE
                     QTLAALRALAAQPAGTGGAPHGPRLRVGAAARQVGVRVSALRFWEQQGLLLPVRDPHS
                     GYRLYHEPQMRRLRVVVLLRAAGYDFPAIRGALDALAAGRTETAIAAVERRRAELART
                     SWACLAALGAFQAYVTEFWADLGGAL"
     gene            complement(1482..2543)
                     /locus_tag="VF276_00100"
     CDS             complement(1482..2543)
                     /locus_tag="VF276_00100"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX6605291.1"
                     /translation="MVELADFDAARLARAGAVAGEAVAAGVYGGVVVAVGNPAGTLWM
                     HTAAESAAVDLDTIFPVASVTKPIVALAVMQLAEAGRLLLNDPVARVIPEFGQQGKDQ
                     ITVWHLLTHTSGLPDDLDDLFAQRAPATAFLRAACERGVRFAPGSHYAYCNVSFVVLG
                     ELIARLSGQPYPDYLRDHIFAPAGMASTGFDPADRARTAAVVFEDGPEEQAYFRTLAA
                     PYGGLWTTANDLLAFGRALLSGGPPDAPLLSPAALAAMTRVQPGLTSFDTGKPVYAAL
                     GWGKRSPMGVLLASDASFGHGGSTGAFLWIDPVPALMFVFLAAPLLPGDPDTDRQRDA
                     QRALNAVYGALRAPPSSAS"
     gene            complement(2558..3595)
                     /locus_tag="VF276_00105"
     CDS             complement(2558..3595)
                     /locus_tag="VF276_00105"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX6605292.1"
                     /translation="MEQAQADLRLLLQETVARYVAPEATVVAVAPAPMAAHELGWSGA
                     EVRRYRVALDSGDSLTLITKTMPLKERRVLVLLSEQGHANVPFTHTLDLTTDAPALAC
                     MQDVGGDPGPLPPDLDARVARALAAIHYRNLGQGDRLPWLPRADYAYFADFLIPEVWR
                     AHWDTALASPPFAEEFAAYTPRLEAAAAHFVAAMDMLWDEGTSLTVTHGDMHPADGSH
                     IITQGGTPYLIDWGWTYYGSFYLDLPNYFTPATVHHYRAALADLGLAIPEAAFMARYH
                     AAARYVGFKYLCAGIWQWPPGPTRQTGRMLLNMLRRALDGAPPDSGFTLAGATWDGLL
                     AAHMQFTGQPD"
     gene            complement(3727..>4054)
                     /locus_tag="VF276_00110"
     CDS             complement(3727..>4054)
                     /locus_tag="VF276_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605293.1"
                     /translation="NGQLVGIGTLTGTITNLLGQVIGAVTNTAVTLPLAASGTCQILH
                     LDLGPLDLNLLGLHVHLSEVVLDITAQQGGGLLGDLLCGVANLLNSGAPLSAIAQVLN
                     RILAIL"
BASE COUNT          633 a         1351 c         1457 g          613 t
ORIGIN      
        1 tgatcactga ccactgacca ctcgtcgtca ctcagcactc agtactcagc actcagcact
       61 cgcccgtgcc gggtgcgctt gcccacggcc aggtcgtacg cctcggcgag gagcggctgc
      121 acctgggtcg cgaacgtggc cgggctgggg ttgaggatgc agacccagta ttgccgccca
      181 tacaccgggt gcggcagcac ctggtccagg gccgtgaagt catagcccgg cgccgcctcc
      241 tcgccggcgc gggcgggcgg gggaccgaac agggcccgga aggtggcctt gccgatgccg
      301 atgttcaggc ggaaggcgcc cggacggttg agattggaga acgggtcgaa agcatcgctg
      361 gtcaccaggg tggcgaacgg gaagcggtga tcgggttcca ggtcgggcgg gttgtagaaa
      421 tagaaggtgg cgccttgcgt ctcggccagg tccacgccgg gaaacgtcgc ggtgatgtac
      481 tgggtgatcg cggcttcgtc catgagcagg ggtcctcctg ttggctgtgc ccgctccgcc
      541 cgtgtggggg tgcgggcctt cgtgtgtgat ggtccaggcc ggccggcgtt ttggactccg
      601 attctattgt ataactcaga tgcaatagta gagggttgga caggagcgtt cggcgcgccg
      661 attctcctgg caggcacgaa acccgcccca cacagccggc ggatgcggta caatgggagc
      721 ggcaagtcgc taaaaccact tgagggtttt acatgatagt tgaggccacg ctgcggacga
      781 tcgacctggc gcgggcgggg cggatcagcg tccagcaggt gcgaaactac gaagccgcgg
      841 ggttgattcc gcccgccgcg cgcggcccta atgggtatcg ccgctacacg cgcaaacacc
      901 tggtcgcgct tacgaccgcg cgggcgctgg tgggcagcta tgggccactg cacgcggcgg
      961 cactgatgca ggcggtccac cggggcgacc tgcccgccgc gctcgcgctg atcgacgcgc
     1021 gccacgccga attggccgcg acgcggcagc aggtggagca aacgctggcc gccctgcgcg
     1081 cgctggccgc gcaaccggcg ggcaccggcg gtgcccccca cgggccgcgg ttgcgcgtgg
     1141 gcgcggcggc ccggcaggtg ggggtgcgcg tctcggcgct gcgcttctgg gagcagcagg
     1201 gcctgctgct ccccgtccgc gatccgcaca gcggctatcg cctctatcac gagccgcaga
     1261 tgcgccgcct gcgcgtggtg gtcctgctgc gcgcggcggg ctacgatttc cccgccatcc
     1321 gcggggcgct tgatgcgctg gccgcgggcc gcaccgagac ggccattgcc gcggtggaac
     1381 ggcggcgcgc cgagctggct cggaccagtt gggcctgcct ggccgcgctg ggcgcgtttc
     1441 aggcctatgt caccgagttc tgggcggacc tgggcggcgc gttatgaggc cgacgatggc
     1501 ggcgcccgca aggcgccgta cacggcgttg agcgcgcgct gggcgtcgcg ctgccggtcc
     1561 gtgtcggggt cgcccggcag cagcggggcg gccaggaaca cgaacatcag cgcgggcacc
     1621 gggtcgatcc agaggaaggc gcccgtgctg ccgccgtgcc cgaacgaggc gtcggaggcc
     1681 agcagcacgc ccatgggact gcgcttgccc cagcccagcg cggcatacac cggcttgccc
     1741 gtgtcgaagc tggtcaggcc gggctgcacg cgggtcatgg cggccagggc ggcggggctg
     1801 agcaacggcg catccggcgg cccgccgctg agcagggcgc gcccgaaggc cagcaggtcg
     1861 ttcgccgtgg tccagaggcc gccgtagggc gcggccagcg tgcggaagta ggcttgctcc
     1921 tccgggccgt cctcgaagac caccgccgcc gtgcgcgccc ggtcggcggg gtcgaagccg
     1981 gtgctcgcca tgcccgcggg cgcgaagatg tggtcgcgca ggtaatcggg atagggctgc
     2041 ccgctcagcc gggcgatcag ttcgcccagc accacgaacg acacgttgca gtatgcgtag
     2101 tggctgccgg gcgcgaagcg cacgccgcgc tcgcaggcgg cgcgcaggaa ggcagtggcc
     2161 ggcgcccgct gcgcgaagag gtcgtccagg tcgtcgggca ggccgctggt gtgcgtcagc
     2221 aggtgccaga cggtgatctg gtctttgccc tgttgcccga actcggggat gacgcgggcc
     2281 accgggtcgt tcagcagcag ccgccccgcc tccgccaact gcatcacggc cagcgccacg
     2341 atcggtttgg tgacggaggc cacggggaag atggtgtcca ggtccacggc ggcgctctcc
     2401 gccgccgtgt gcatccagag tgtgcccgcg gggttgccca ccgcaaccac cacgccgcca
     2461 tacacccccg cggccaccgc ctcgccggcc acggctccgg cccgcgccag ccgcgcggca
     2521 tcgaaatcgg ccagttcgac catgcgctcc tcccttgcta atccggttgc ccggtgaact
     2581 gcatatgcgc ggccagcagg ccgtcccagg tcgccccagc cagggtaaag ccgctgtccg
     2641 gcggggcacc gtcgagcgcg cgccgcagca tgttcagcaa catgcgcccc gtctgccggg
     2701 tggggccggg cggccactgc cagatgcccg cgcagagata cttgaagccc acgtaccgcg
     2761 ccgccgcgtg gtagcgcgcc atgaaggcgg cttcggggat tgccagtccg agatccgcca
     2821 gcgcggcccg gtagtggtgg acggtggcag gcgtgaaata gttcggcagg tccaggtaga
     2881 acgagccgta gtaggtccag ccccagtcga tcaggtaggg ggtgccgccc tgcgtgatta
     2941 tatggctgcc gtcggcgggg tgcatatcgc cgtgcgtcac ggtcagcgaa gtaccctcgt
     3001 cccagagcat gtccatggcc gcaacgaagt gcgcggcggc agcctccagg cgcggcgtat
     3061 aggcggcgaa ctcctcggcg aaaggcgggc tcgccagcgc cgtatcccaa tgggcgcgcc
     3121 agacctccgg gatgaggaag tccgcgaagt aggcatagtc ggcgcgcggg agccagggca
     3181 gccggtcgcc ctggcccagg ttgcggtaat gaatggcagc cagagcgcgc gccacccgcg
     3241 catccaggtc gggtggcagc ggaccggggt caccgcccac atcctgcata caggcgaggg
     3301 ccggggcgtc ggtagtcagg tcgagggtgt gggtaaacgg cacattggcg tggccctgct
     3361 cgctgagcag caccagcacc cggcgctcct tcagcggcat cgtcttggtg atgagagtca
     3421 gcgaatcgcc gctatcgagt gccacccggt agcggcgcac ctccgccccc gaccagccga
     3481 gctcatgcgc ggccatcggc gcaggggcca ccgctaccac cgtggcctcc ggcgcgacat
     3541 accgcgccac cgtctcctgc aacaacagcc tcagatccgc ctgcgcttgc tccactctgg
     3601 tgcccctttc gggcttatga taccacagag tgtgccggac acaaagaaag ggaggaagtg
     3661 acatgccact tcctctccgt tggctctatt cggttgtact ggttatgctc gggctgatgc
     3721 tggatgctaa agtatcgcta gaatacgatt cagcacctga gcgatggcgc tcaacggcgc
     3781 gccgctgttc aacaagttgg caacgccgca cagcagatcg ccgagtaagc cgccaccctg
     3841 ttgggccgtt atgtcgagca cgacctcact gaggtggaca tgcagaccga gcaggtttag
     3901 atccagcggg ccgagatcca ggtgcaggat ctggcaggtg ccggaagccg cgagcggcag
     3961 ggtaaccgcc gtgttcgtca ccgcgccgat cacctgcccc aacaggttgg tgatcgtccc
     4021 ggtgagcgtc ccaattccga ctaattggcc gttc
//
LOCUS       DASUYC010000008         2108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1614139, whole genome shotgun sequence.
ACCESSION   DASUYC010000008 DASUYC010000000
VERSION     DASUYC010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1614139"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1719)
                     /locus_tag="VF276_00115"
     CDS             complement(<1..1719)
                     /locus_tag="VF276_00115"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX6605294.1"
                     /translation="MNTVHAFLRPFTAFLAFGVTVAGALLANDQLWLVCLGVIFLCLL
                     YLVWSLVPPVAPRARAALAGPASAEDREQARNEALSHSVQMLAGFLLAGFVLLSAHLL
                     REQFVQAGAIAGSRVLNETVQTGPDQYQQDTTLIRNGNPITFTRTIEASGVQTGTDSL
                     QNPRLLARDLRVQRGQIFDAAGNIIAGRTVYSDTGYVERTYPAPNMSYLLGYYNPTIY
                     GLAGMEANLDPYLSGEAGSNPILAEEDRILHRPTVGSDVYLTLVPAIQNAATQALGQR
                     KGGVVVIDIPTGAILALVAYPHIDPNGLAFNPNNDWTEENQRIIDYWNTVTARADLPM
                     LNRATQGLYPPGSTFKTVTLAAALDMGKVQPTTVFTDTGRIKVEAGNYFHVDCSTCRP
                     AGHPSDLFTLMEGYQWSLNVVFAELATRYLGTDPLVRYAQGFGFGRDYNENNPMLAIP
                     VAASRLGDPDFLKTPNGIAATSYGQGQVQATPFEMAMVAATVGHGGELPEPYLVSKVV
                     NPKGDTVEQTRPGTLGRAVSPATDAVLTQMMITSVQKGWANGAAIPGYTVGGKTGTAE
                     TGRGTSH"
     gene            complement(1728..>2108)
                     /locus_tag="VF276_00120"
     CDS             complement(1728..>2108)
                     /locus_tag="VF276_00120"
                     /inference="COORDINATES: protein motif:HMM:NF022359.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2695 domain-containing protein"
                     /protein_id="HEX6605295.1"
                     /translation="LPHQHQPDPHHSNNDAAMPLEDDEEWEEVPVTETLMEALADVIS
                     MDPETGEIAGCADDFRVTQEVLTRHGQEHVAEHVLALLRAQGAMCDCGVLRYSQIDLV
                     FLEDDLEEEDLEEYEEEDEEEMAT"
BASE COUNT          305 a          659 c          753 g          391 t
ORIGIN      
        1 gtgcgaggtg ccgcggcccg tttcggcggt gcccgtcttg ccgccgactg tgtagccggg
       61 gatggccgcg ccgttcgccc accccttttg caccgacgta atcatcatct gcgtgagcac
      121 cgcgtccgtg gccggggaga cggcgcggcc cagcgtgccg gggcgggtct gctccacggt
      181 gtcgcccttg ggattgacga ccttgctgac gagatagggc tccggcaact cgccgccgtg
      241 gccgacggtg gccgccacca tcgccatctc gaacggggtg gcctgcacct ggccctggcc
      301 gtagctggtg gcggcgatgc cgttgggcgt cttgaggaag tcgggatcac ccaggcggct
      361 ggcggcgacc gggatcgcca gcatggggtt attctcgtta tagtcgcgcc caaagccgaa
      421 gccctgcgcg tagcgcacca gcgggtcggt gcccaggtag cgtgtcgcca gttcggcgaa
      481 gacgacgttc agcgaccact ggtagccttc catcagcgtg aacaggtcgc tggggtggcc
      541 ggcgggccgg caggtcgaac aatcgacatg gaagtagttc ccggcctcga ccttgatccg
      601 ccccgtgtcg gtgaacacgg tggtgggttg gaccttgccc atgtccagcg cggcggccag
      661 ggtgacggtt ttgaacgtcg accccggcgg gtagaggccc tgcgtggccc ggttcagcat
      721 cggcaggtcg gcgcgcgccg tgacggtgtt ccagtagtcg atgatgcgct ggttctcctc
      781 ggtccaatcg ttgttggggt tgaaggccag cccgttcggg tcgatgtgcg gataggctac
      841 cagggccagg atggcgccgg tggggatgtc gatgacgacc acgccgccct tgcgctggcc
      901 cagcgcctgg gtggcggcgt tctggatggc gggcaccagc gtcagataga catccgagcc
      961 gaccgtgggg cggtgcagga tacggtcctc ctcggccagg atcgggttgc tgcccgcctc
     1021 gccgctcaga taggggtcga ggttggcttc catgccggcc aggccgtaga tggtcggatt
     1081 gtagtagccg agcaggtagc tcatattggg cgccgggtag gtgcgctcga cgtagccggt
     1141 gtcggagtag accgtgcgcc cggcaatgat gttgcccgcg gcgtcgaaga tctgtccgcg
     1201 ctgcacgcgc aggtcgcgcg ccagcaagcg cgggttttgc agcgagtcgg tgcccgtttg
     1261 cacgccgctg gcctcgatgg tgcgggtgaa cgtgatcggg ttgccgttgc ggatcagggt
     1321 cgtgtcctgc tggtactggt cggggccggt ctgcacggtt tcgttgagca cacggctgcc
     1381 ggcgatggcg cccgcctgca cgaactgctc gcgcagcagg tgggcgctga gcagcacaaa
     1441 gccggccagc aggaacccgg ccagcatctg cacgctgtgg ctcagtgcct cgttgcgggc
     1501 ctgctcccgg tcctcggcgc tggcagggcc ggccagggcg gcgcgggcgc gcggggccac
     1561 cggcggcacg aggctccaga ccaggtacag caggcagagg aatatcacgc ccaggcaaac
     1621 gagccagagc tggtcattgg cgagcagcgc tccggccacc gtcaccccga aggcgaggaa
     1681 ggcggtaaag gggcgcaaga aagcgtgtac ggtattcatg ccccggttca ggtcgccatt
     1741 tcctcttcgt cttcttcttc gtactcttcg agatcctctt cttcgaggtc atcttccagg
     1801 aagacgaggt cgatctggct gtagcgcagc accccacagt cgcacatggc gccttgcgcg
     1861 cgcagcaagg ccagcacgtg ctcggcgacg tgttcctggc cgtgccgggt gagcacttcc
     1921 tgagtgacgc ggaaatcgtc ggcacagccc gcgatctctc cggtctccgg gtccatgctg
     1981 atcacatcgg ccagcgcctc catcagcgtt tcggtaacgg gcacctcttc ccattcctcg
     2041 tcgtcttcca gtggcatcgc cgcatcgttg ttcgagtgat gcgggtcggg ttggtgctga
     2101 tgcggcaa
//
LOCUS       DASUYC010000009         2904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_688955,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000009 DASUYC010000000
VERSION     DASUYC010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688955"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /gene="rsmH"
                     /locus_tag="VF276_00125"
     CDS             <1..633
                     /gene="rsmH"
                     /locus_tag="VF276_00125"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX6605296.1"
                     /translation="DTPARGFSFLAEAPLDMRMDPTASGQPPAADLVNQLPEGELADL
                     IYRYGEERDSRRIARRIVAERQVAPIETTSRLAAIVLAAVGGRPAGPKGRIHPATRTF
                     QALRIAVNRELDVLPGALDAAVGLLKPGGRLAVIAFHSLEDRIVKQFIQQEVKGCICP
                     PELPVCQCGRKPRLRTISKGAIQAAAAEVESNPRARSARLRVAERLPGGP"
     gene            844..1302
                     /locus_tag="VF276_00130"
     CDS             844..1302
                     /locus_tag="VF276_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605297.1"
                     /translation="MALRIAVPNFPLEQEEPTPNPSPVSDARERRIKLDAYLALDPAI
                     RFLIALALVAAISLLYLVQASTVTELNYDVQRQAVEYDKLVRERQQLQLQIAHAQALP
                     QIEQIARTKLKMVPVGDAYRYLQVPPAAGNTSGATTPPAAGAPPAAGAQP"
     gene            1434..>2904
                     /locus_tag="VF276_00135"
     CDS             1434..>2904
                     /locus_tag="VF276_00135"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX6605298.1"
                     /translation="MPQSQTLIRGWRIWAVLVFFVLGAGYIAVHLFKLQVLQQPVFAK
                     SAAARITWTDTILPNRGVIRDSRGFLLAGNAVASDLYLSTSNRTDADLHTIADLLGPI
                     LSQAPEDLYQQIKGQTATTQLLYRRLDSEAAARVLQLRNTWPVVRSAIWLEDQPRRDY
                     PNGNFAAHILGFADYDNHGQYGVEQFYDDRLAGVPGSLTAERDSQGYPLPLGDASQKQ
                     AVDGADLDLTIDSAVQYMAQRELDNTLKQTGAASGQILIMDPHTGAIVALATSPRFDP
                     NHFTDVAQDQYDIFKNPAVVDVYEPGSTFKVLTYASALDAGVITPDTTYNSTGVFMAY
                     GVPIYNSIKAGYGLETMREGLARSDNNAADFAADQLGEKKFYEYIKNFGIGQETGVDM
                     AGEVAGLLAYPGDDGYSPVNLYTNAFGQGLAVTPLQLVTAISAVANGGNLMKPYVVSR
                     VSRNGQVLQENKPAVVRRVISPESARTITDMLAWAVDTTP"
BASE COUNT          515 a          990 c          939 g          460 t
ORIGIN      
        1 gacacccccg cgcggggatt ctccttcttg gccgaggccc cgctggacat gcgcatggac
       61 cccaccgcgt ccgggcagcc cccggccgcc gaccttgtca atcagttacc cgaaggggaa
      121 ctggcggatt tgatttaccg ctacggcgag gagcgcgact ctcgccgtat cgcccggcgc
      181 atcgtagccg agcgacaggt cgccccgatc gaaaccacca gcaggctggc cgcgattgtc
      241 ctggcagcgg tcggcggccg gcctgcaggg cccaaagggc ggatccaccc cgccacgcgc
      301 acctttcagg cgctgcgcat cgccgtcaat cgtgagcttg acgtgttgcc cggcgcgctg
      361 gacgcggcgg tgggtctcct gaagcccggt ggacgcctgg cggtgatcgc cttccactcg
      421 ttggaagacc gcatcgtcaa gcagtttatc cagcaagaag tgaagggttg tatctgtccg
      481 ccggaattgc cggtctgcca atgcgggcgc aagccccggt tgcggaccat cagtaaaggc
      541 gcgatccagg ccgcggcggc cgaggtcgag agcaacccgc gtgcccgcag cgctcgcctt
      601 cgtgtggccg agcgcctgcc cggcgggcca tagcgtccgg cctcgtgccg ggcatatgat
      661 tagaagccgg gagatcacgc gggggggtac ccagcgggat ctctttttta ttggccggcg
      721 cacggggcct cttgacccca ggtgctataa tcggtataga acggaagttc tatcgacgct
      781 cgcagcgcgg ccggcaggcc gcgttcgtcc ctactgatca cgatccagca aaggtgacac
      841 gcgatggcgc tacgaattgc agtaccgaac ttcccgttgg agcaggaaga gccgactccg
      901 aaccccagcc cggtttcgga tgctcgcgaa cgccggatca agctcgacgc ctacctcgcc
      961 cttgatccgg cgattcgctt cctgattgcc ctggccctgg tcgcggcgat cagcctgctc
     1021 tacctggtgc aggccagcac agtcacggag ttgaactatg acgtgcagcg ccaggcggtg
     1081 gagtacgaca agctggtgcg cgagcgccag caactgcaac tccagatcgc gcacgcgcag
     1141 gcgttgccgc agatcgagca gatcgcgcgc accaagctga agatggtgcc cgtgggcgac
     1201 gcgtaccgct atttgcaggt gccgcccgcc gcgggcaaca ccagcggggc gaccaccccg
     1261 cccgccgcgg gggcgccacc ggcggcgggg gcgcagccat agcggcccgg cccgcgcaac
     1321 acgatccggg cgcaagcccc gcgagaggga tccgggccgg tcgatccggc ccggatccct
     1381 gagccattct tgtcgaacgt catgtgaggc cctcggggcg ctgaggtgga acgatgccgc
     1441 aatcgcaaac tctgatccgc ggatggcgca tctgggccgt actggtcttc ttcgtgctgg
     1501 gcgcgggcta tattgcggtg catctgttca agctgcaggt gctccagcag cccgtctttg
     1561 ccaagagcgc ggcggcgcgc atcacctgga ccgacaccat cctgcccaac cggggcgtga
     1621 ttcgcgattc ccgcggcttc ttgctggccg gcaacgccgt ggcctcagat ctctacctga
     1681 gcaccagcaa ccgcaccgac gccgatctgc acaccatcgc cgatctgctc ggtcccatcc
     1741 tctcccaggc gcccgaagac ctctatcagc agatcaaggg ccagaccgcg acgacccaac
     1801 tgctctaccg gcggctggac agcgaggccg ccgcccgcgt gttgcagttg cgcaacacct
     1861 ggccggtggt gcgtagcgcc atctggctcg aagaccagcc gcggcgcgac tatcccaacg
     1921 gcaacttcgc cgcccatatc ctcggcttcg cggactatga caaccacggc cagtacggtg
     1981 tggagcagtt ctacgacgac cggctggccg gcgtgcccgg ctcactcacg gcggagcgcg
     2041 actcgcaggg ctatccgttg ccgctgggcg acgccagcca gaagcaggcc gtggatggcg
     2101 ccgacctgga cctgaccatc gacagcgcgg tgcagtacat ggcccagcgc gaactcgaca
     2161 acacgctcaa gcagaccggc gccgccagcg gccagatctt gatcatggac ccgcacaccg
     2221 gggccatcgt cgccctggcg acctcgccgc gcttcgaccc gaaccacttt accgatgtcg
     2281 cgcaggacca gtacgacatc ttcaagaacc cggcggtggt cgatgtctac gaacccggct
     2341 cgacgttcaa ggtgctgacc tacgcctcag ccctcgacgc cggggtgatc acgccggaca
     2401 cgacgtacaa ctccaccggc gtattcatgg cctacggtgt gccgatctac aacagcatca
     2461 aggccggcta cgggctggag acgatgcgcg aggggctggc gcggtccgac aacaatgccg
     2521 cggacttcgc cgcggaccaa ctgggcgaga agaaattcta tgagtatatc aagaatttcg
     2581 gcatcggcca ggagaccgga gtggatatgg cgggcgaggt ggccgggttg ctggcctatc
     2641 ccggcgacga cggctattcg ccggtcaacc tctataccaa cgccttcggg cagggcctgg
     2701 ccgttacgcc gctgcaactg gtcacggcga tctcggcggt cgccaacggc gggaacctga
     2761 tgaagcccta tgtcgtgtcg cgggtctcgc gcaacggcca ggtgctgcaa gagaacaagc
     2821 cggcagtggt gcgccgggtg atcagcccgg agtcggcgcg gacgataacc gatatgctgg
     2881 cctgggcggt ggacaccacg cccg
//
LOCUS       DASUYC010000010         2915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1613541, whole genome shotgun sequence.
ACCESSION   DASUYC010000010 DASUYC010000000
VERSION     DASUYC010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1613541"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="VF276_00140"
     CDS             <1..990
                     /locus_tag="VF276_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014173077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX6605299.1"
                     /translation="IGHGHSHGAVQIDPALEGSTEGIQAIRLSLLILGATAVVQAIIA
                     LLSGSAGLLADTIHNGADALTALPLWIAFTLTRRPPNRRFTYGYDRAEDVAGVAIVLI
                     IFVSAGVALWESVDKLIHPVPLTNVWWVIAAAAGGFAGNEWVANIRTRTGERIGSAAL
                     VADGQHARADGLTSLAVLVGAAGALLGFPILDPLVGLLITLAILVIGKEAGQAILYRL
                     MDAVDPELVDQVEHEAAHVAGVRATHDVRIRWHGHRLIAELHADVDPALSTVDSHEIG
                     EQVRHALLHKIAGLSEVLVHIDPAGHAPEYYHQTTWHHRRPTGGTAPPQAHTH"
     gene            1262..>2915
                     /locus_tag="VF276_00145"
     CDS             1262..>2915
                     /locus_tag="VF276_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HEX6605300.1"
                     /translation="MAEQQTDTAAYDVRLITDGANGGTVPAPARNDPTKREPPPSALA
                     LRPQSSATRHPTLETLLKLVRQYSQPKDEDLEAIERAYHFAARAHEQQKRQSGEPYIL
                     HPLAAAIILAEMRLVDPETLQAALLHDVLEDNPDISKAMIQEEFGEGVARLVDSVTKL
                     STLPKTALFEDPERRENAKKQEQAETLRKMLMAMFDDMRVVLIKLADRLHNMRTLDCT
                     PPDKQRRIAAQTLEIYAPLANRLGIWQIKSELEDLAFFYLEPEKYRDLVAQVQERKDS
                     STRYIQRVIFHLQRALKEAGIECEIKGRTKHIYSLYQKMLRKNRPSDRIYDLLAVRII
                     VNEIQDCYAALGVIHTLWPPIFGEFDDYIAVPKESMYQSLHTAVWAFDNKPLEIQIRT
                     HKMHETAEFGIAAHWRYKEGQRAARRDREYETKIASLRRQMDWRHDIEDAQEFVESLH
                     SDILQDHIYVYTPKGDLIELPAGATPIDFAFRIHTELGYQCSGATANGKIITLDTPLK
                     NGDMVKVIKDPRRKGPSRDWIQSGRRYAVTANARQKISQWFRK"
BASE COUNT          587 a          991 c          889 g          448 t
ORIGIN      
        1 atcggccacg ggcatagcca cggcgcggtc cagatcgacc cggcgctcga aggcagcacc
       61 gaaggcatcc aggccatccg cctgagcctg ctgatcctcg gcgctaccgc cgtggtccag
      121 gcgatcatcg ccctgctgag cggcagcgcc ggcctgctcg ccgacacgat ccacaacggc
      181 gccgatgccc tcaccgccct gcccctgtgg atcgccttca ccctgacccg ccggcccccc
      241 aatcgccggt tcacctacgg ctacgaccgg gccgaagacg tggccggcgt cgccatcgtg
      301 ctgatcatct tcgtcagcgc cggggtggcc ctctgggagt cggtggacaa gctgatccac
      361 ccggtgccgc tgaccaacgt ctggtgggtg atcgcggcgg ctgcgggcgg cttcgcgggc
      421 aacgagtggg tggccaatat tcgcacgcgc acgggagaac gcatcggcag cgcggccctc
      481 gtcgccgatg ggcagcacgc ccgcgccgac ggcctgacct cgctggccgt gctggtcggc
      541 gccgccgggg cgctgctggg cttccccatc ctcgacccgc tggtcggcct gctgatcacg
      601 ctggcgatcc tggtcatcgg caaggaggcc gggcaggcca tcctctaccg cctgatggac
      661 gccgtcgatc ccgaactggt cgaccaggtg gagcatgaag ccgcgcatgt ggcgggcgtg
      721 cgcgccacgc acgatgtgcg gattcgctgg catgggcacc ggctgatcgc cgaactgcat
      781 gcggatgtcg atcccgccct ttccaccgtg gacagtcacg agattggcga gcaggtgcgc
      841 cacgccctgc tgcacaagat cgccggcctg agcgaagtgc tggtgcatat cgaccccgcc
      901 gggcacgcgc ccgagtatta tcaccagacc acctggcatc accggcgccc caccggggga
      961 accgcgcccc cgcaggcgca cacccactaa cggcgggcac gccgggatga tagcgcaggc
     1021 gcggcaggga aaacaatggc cgaccggccc cacgcgaacc gcgaatcagg gcggttggtc
     1081 ccttacacat gtgtgctggg ctgcgaactg aattgacacg atcccgccga tgcggtatag
     1141 ttaatagaag cgatgcctgg gcgggaggct gctatagttg tttgccggga gtcggagact
     1201 cccgcatttt ttcccccgcc accgggtcca caatatcatt gaaataataa ggaggtgcgc
     1261 catggctgag cagcaaaccg acacggctgc ctatgatgtg cgcctcatca cggacggcgc
     1321 caacggcggg accgttcctg caccggcgcg gaatgaccct acgaaacgcg aaccgccgcc
     1381 ctcggcgctg gccctgcggc ctcagtcgtc cgccacccgg caccccacgc tggaaaccct
     1441 gctgaagctg gtccgccagt actcccagcc gaaagatgag gatctggagg cgattgagcg
     1501 cgcctaccac ttcgcggcgc gcgcgcacga gcagcagaag cgccagagcg gcgaacccta
     1561 tatcctccat cccctggccg ccgccattat cctggccgag atgcgcctgg tcgacccgga
     1621 aacgctgcag gcagcgctgt tgcacgacgt gctggaggac aacccggaca tcagcaaggc
     1681 gatgatccag gaggaattcg gcgaaggagt tgcgcgcctg gttgacagcg tcaccaaact
     1741 gagcacgctg ccgaaaacag ctctgtttga ggatccggag cggcgggaga acgcgaagaa
     1801 acaagagcag gccgaaaccc tccgcaagat gctcatggcg atgtttgacg atatgcgggt
     1861 ggtgctgatt aagctggccg accgcctcca caacatgcgc accctcgact gtaccccgcc
     1921 cgacaagcaa cggcgcatcg ccgcccaaac actcgaaatt tacgcacccc tcgcaaaccg
     1981 cctcggcatc tggcagatca aaagtgaact cgaagacctc gctttcttct acctcgaacc
     2041 tgaaaaatac cgggacctcg tcgcgcaagt ccaggaacgc aaagattcga gtacccgcta
     2101 tatccagcgg gtgatcttcc atctgcaacg ggccttgaaa gaggcgggca tcgagtgcga
     2161 aatcaagggc cggaccaagc acatctattc gctgtatcag aagatgctgc gcaagaaccg
     2221 gccatccgat cgcatttacg atctgctcgc cgtgcgcatc atcgtcaacg agatccagga
     2281 ctgctacgcg gcgctgggcg tcatccatac gctctggccg ccgatcttcg gggagttcga
     2341 cgattatatc gccgtgccca aagagagcat gtaccagtct ttacataccg ccgtctgggc
     2401 gttcgacaac aagccgctcg aaatccagat ccgcacacac aaaatgcacg agacggccga
     2461 gttcggcatc gcggcccact ggcgctacaa ggaaggccag cgcgccgccc gccgcgaccg
     2521 cgaatacgag acgaagatcg ccagcctgcg ccgccagatg gactggcggc acgacatcga
     2581 ggacgcgcag gagtttgtcg agtcgctgca ttcggatatt ttgcaggacc acatctacgt
     2641 ctacacgccg aagggcgatc tcatcgagtt accggcgggt gccacgccca tcgacttcgc
     2701 cttccgcatc cataccgagc tgggctacca gtgcagcggg gccaccgcca acggcaagat
     2761 catcaccctc gacacgccgc taaagaacgg cgacatggtc aaggtgatca aggacccgcg
     2821 gcgcaagggc cccagccgcg actggatcca gtcggggcgg cgctatgccg tcacggccaa
     2881 cgcgcggcag aagatcagcc agtggttccg caagc
//
LOCUS       DASUYC010000011         2010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1489696, whole genome shotgun sequence.
ACCESSION   DASUYC010000011 DASUYC010000000
VERSION     DASUYC010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1489696"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2010
                     /locus_tag="VF276_00150"
     CDS             <1..>2010
                     /locus_tag="VF276_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX6605301.1"
                     /translation="DGTQVRKIVDWVTYTDPAGDDDPTWINMQDQVTASGGVFTYHGV
                     TYTAPANGTYRIGLFNERDSRLGGELGNDVNRDGNPAGSSGIFAVLWNTTSNNVWVDT
                     NQNRNFADQAGMTDYKVRYDIGTFGTDNPATAIRESVPFVVQTDGRNKYVNIGIVSGA
                     HGTHVAGIATGKGFFGGAFNGAAPEAQIVSIRVCLFVSGCTAHALVEGMIYAEKQANV
                     DVINMSIGGLPALNDGNNTRAVIYNRLITQYKAQMFISAGNDGPGVNTIGDPAVATNV
                     MAIGAYVTKDSWMANYGADAAKDEGLFVFSSRGPREDGGFKPNIVAPGSAVSSVPAWQ
                     PGQPVGGTYNLPPGYGMFNGTSMAAPQATGGAALLISAAKQAGAQWKPDQLRQAINSS
                     ARFIAGYGAYEQGNGLFRVGAAWDLLKTNIKTVGITSSAPVNTIISQFLATPNVGTGI
                     YEREGWKAGDTGTRTVTFTRTTGGSRAITYNLTWVGNDGTFSSAGSIALPLNRPVSLP
                     VTVHPTGDGVHSAILNLDDPATTGVDYGVLNTIVVAGQFSAGNNFTVSYNGSADRPDK
                     ASFFFYVPANTPAFKVDVTNVNGRVRLLRFHPYGISIDNTSTTPYATNSNQSRTLSNP
                     QAGVWEVTVDTSRTSPNSPATFTVAGTILGVDINPTSWVINSATVG"
BASE COUNT          392 a          709 c          598 g          311 t
ORIGIN      
        1 gacggcacac aggtccgcaa aatcgtcgat tgggtgactt acacagatcc cgccggcgac
       61 gacgatccga cctggatcaa catgcaggac caggtcacgg ccagcggcgg cgtcttcacc
      121 tatcacggtg tgacttacac cgcgcccgct aacggcacgt accgcatcgg tctgttcaac
      181 gaacgggact cgcggctggg cggcgaactc ggcaacgacg tgaaccgcga cggcaatccg
      241 gccggcagca gcggcatctt cgccgtgctg tggaatacca ccagcaacaa tgtctgggtg
      301 gacacgaacc agaaccggaa ttttgccgac caggcgggga tgactgatta caaggtgcga
      361 tatgacatcg gcaccttcgg gaccgataac ccggccacgg cgatccgcga aagcgtgcct
      421 ttcgtcgttc agactgacgg gcggaacaag tacgtcaaca tcggcatcgt ctccggtgcg
      481 cacggcacgc atgtcgccgg catcgccacc ggcaagggct tcttcggcgg ggccttcaac
      541 ggcgcggcgc ccgaagcgca gatcgtgtcg atccgtgtct gcttgttcgt ctccggctgc
      601 accgcgcacg ccctggtcga gggcatgatc tacgccgaaa agcaggccaa tgtcgacgtg
      661 atcaacatga gcatcggcgg cctgcccgcg ctgaacgacg gcaacaacac tcgcgccgtc
      721 atctacaacc ggctgatcac ccagtacaag gcgcagatgt tcatctccgc cggcaacgac
      781 ggccccggtg tcaacaccat cggcgacccg gcggtggcga ccaatgtgat ggctatcggc
      841 gcttacgtaa ccaaagatag ctggatggcg aactacggcg cagatgccgc caaggacgaa
      901 gggctgttcg tattctcgtc gcgcggcccg cgtgaggacg gcggatttaa gcccaatatc
      961 gtcgcccccg gctcggccgt ctccagtgtt ccggcctggc agccggggca gccggtcggc
     1021 ggcacctaca acctgccgcc tggctatggc atgttcaacg gcacctcgat ggcggcgccg
     1081 caggccaccg gcggcgcggc cctgctgatc agcgcggcca agcaggccgg cgcccagtgg
     1141 aagcccgacc agttgcgcca ggcgatcaac tccagcgccc gcttcatcgc cggctacggc
     1201 gcctatgagc agggcaacgg cctgttccgg gtcggcgccg cctgggatct gctcaagacc
     1261 aacatcaaga cggtcggcat caccagcagc gccccggtga acacgatcat cagccagttc
     1321 ctggcgactc ccaacgtggg caccggtatc tatgagcgcg aaggctggaa ggccggtgac
     1381 acgggcacgc gcacggttac cttcacccgc accaccggcg gctcccgcgc catcacctac
     1441 aatctgacct gggtcggcaa cgacggcacc ttcagcagcg ccggctcgat tgctctcccg
     1501 ctcaaccggc cggttagcct ccccgtcact gtccatccca ccggggacgg cgtgcacagc
     1561 gccatcctga acctggacga tcccgccacc accggcgtgg actacggggt gctgaacacg
     1621 atcgtcgtcg ccgggcagtt cagcgcaggc aacaacttca ccgtcagcta caacggttcc
     1681 gccgaccggc ccgacaaggc cagctttttc ttctacgtgc cggccaacac cccggccttc
     1741 aaggtcgacg tgaccaacgt gaacgggcgc gtgcgcctgc tgcgcttcca cccctatggc
     1801 atctcgatcg acaacaccag cacgacccct tacgcgacca acagcaacca gagccgcacc
     1861 ctctccaacc cgcaggcggg cgtctgggaa gtgacggtcg atacctcgcg cacctcgccg
     1921 aacagccccg ccaccttcac cgtcgccggc acgatcctgg gtgtggatat caaccccacg
     1981 tcctgggtga ttaactcggc aacggtgggg
//
LOCUS       DASUYC010000012         2776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_53633,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000012 DASUYC010000000
VERSION     DASUYC010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53633"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VF276_00155"
     CDS             <1..442
                     /locus_tag="VF276_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEX6605302.1"
                     /translation="FGVPANTPPPADRTFRDVPADHPFYAAIEAAAHAGIVSGYACGT
                     RPDEPCPGLYFRPYANVTRGQLTKIVAIAGRWTLTNPPAPTFHDVPAESPFYPYIEAA
                     ACRGVISGYADGTFRPGANATRGQTAKIVYNALTAAASCVPAGR"
     gene            498..>2776
                     /locus_tag="VF276_00160"
     CDS             498..>2776
                     /locus_tag="VF276_00160"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX6605303.1"
                     /translation="MLRPPSHPWRARWPAMAVICAALLVTLYQPAPSAGADPGGTRPV
                     SALGAPRVSTAADATANRVAPDLREAVAAASGPDVRVPVIVVSQGPAQDGGLLQNAVA
                     RRPDPHGVVFTSGQARAGQVNALARPANVIAVLSGAPPPSPEPPGLAGSAPVRPDRRA
                     GPQRPTGDLIPRPAPGQKAAAPAGAQAPQSPDSWHTVDVQDVRPAWQAGYRGAGVNVA
                     VVDSGVDFGHPDLQNTFARVEDPASPYYGWPLAFDPTSMALYAGGIVGDIDNAIRAYG
                     SWYIDTSAVIAGSSGAFTTVTAGASGMERITHTYQMPGTSKSGRYHIGIFPDEHLAFD
                     VYGEYPVVVVADTVQAGVYDTVYVDLLNTHDLRNAVALRKGHEVGTVDTTGDGVADLS
                     TGMLYFIADGAHPVPGSDWLYGLSAPDNGTLVAMMGSYDYAEHHGTACASSVVAQGRI
                     DGDNAGLRPPYKPPGAGGMVQGMAPDAKVIAIGNVYRGGMAIYDSYALITYGLDGRPN
                     TGDEPQIASLSFGFSGGIDSGWDFQSRYLAALQKDNPHLSIVVATGNGGPGYGTVTTP
                     ATAPNVISVGAATQYGETTTFEPISSTAEITWGDIQPWSNRGPSILGNEAPSVLAVGA
                     WGTADDALNYSRNGAIAYNIWGGTSMATPITAGVLALGYQAYHLATGQWPTMTLAREL
                     LASSARDLNYGVFSQGAGLVDGKRLVDLAAGQAGLRVSPMRWTPGQQAPAFAGYVYPG
                     GSATTPVTVANPGSTPLTVT"
BASE COUNT          399 a         1119 c          899 g          359 t
ORIGIN      
        1 gtttggggtg ccggccaaca ccccgccccc cgccgaccgc accttccggg acgtgccggc
       61 ggatcacccg ttctacgccg cgatcgaggc cgccgcgcac gcgggcatcg tcagcggcta
      121 cgcctgcggc acgcgccccg acgagccatg ccccggcctc tatttccggc cctacgccaa
      181 cgtcacgcgc gggcaactga ccaagatcgt cgccatcgcc gggcgctgga cgctgaccaa
      241 cccgcccgcc cccacgttcc acgacgtgcc ggcggagtcg ccgttctatc cctacatcga
      301 ggcggccgct tgccggggcg tcatctccgg ctacgccgac ggcacgttcc gccccggcgc
      361 caacgcaacc cgcgggcaaa ccgccaagat cgtctacaac gcgctcaccg ccgccgcatc
      421 ctgtgtgccc gcggggcgct gaacccgccc ctgaccgggc cgtggcctac cggctttagt
      481 ttctagaagg atcgctcatg cttcgccctc ccagccatcc ctggcgcgct cgctggcccg
      541 ccatggccgt gatctgcgcc gccctgctcg tgacgctgta ccagcccgcg ccttctgccg
      601 gcgcggaccc cggcggcacc cggcccgtca gcgccctcgg cgcgccgcgc gtcagcaccg
      661 ccgccgacgc gacggccaac cgcgtcgccc ccgacctgcg cgaggccgtg gccgcggcaa
      721 gcgggcccga cgtgcgcgtg ccggtgatcg tggtcagcca gggaccggcc caggatggcg
      781 ggctgctgca aaacgccgtg gcccggcggc ccgacccgca cggcgtcgtc ttcaccagcg
      841 ggcaggcgcg cgccgggcag gtcaatgccc tggcccgccc cgccaacgtc atcgccgtgc
      901 tctccggcgc gccgccaccc tcgcccgaac cgcccggcct ggccggcagc gcccccgtcc
      961 gccccgaccg gcgcgccggg ccgcagcggc ccaccggcga tctgatcccg cgccccgccc
     1021 ccggtcagaa ggccgccgcc ccggcgggcg cgcaggcgcc gcagagcccc gatagctggc
     1081 acaccgtaga cgtgcaggat gtccgcccgg cgtggcaggc gggctaccgc ggcgcgggcg
     1141 tcaacgtcgc cgtggtggac tcgggcgtcg atttcggcca ccccgatctg caaaacacct
     1201 tcgcccgcgt cgaagacccg gcctcgccct actatggctg gccgctggcg ttcgacccca
     1261 cctcgatggc gctctacgcg ggcggcatcg tcggcgatat cgacaacgcc atccgcgcct
     1321 acggctcgtg gtatatcgac acctcggccg tgatcgccgg cagcagcggc gccttcacca
     1381 ccgtgacggc gggcgcgagc ggcatggaac ggatcaccca tacctaccag atgccgggca
     1441 ccagcaagag cgggcgctac cacatcggca tcttccctga cgagcacctg gctttcgacg
     1501 tgtacggcga gtaccccgtc gtcgtggtgg ccgacacggt gcaggcgggc gtctacgaca
     1561 cggtgtacgt ggacctgctc aacacgcacg acctgcgtaa cgccgtcgcc ctgcgcaagg
     1621 gccacgaggt gggcacggtg gacaccaccg gcgacggcgt ggccgacctg tccaccggca
     1681 tgctctactt catcgccgac ggcgcgcacc cggtccccgg cagcgactgg ctgtacggcc
     1741 tgagcgcgcc cgacaacggc acgctggtcg ccatgatggg ctcgtatgac tacgccgagc
     1801 accacggcac cgcctgcgcc tcgtcggtcg tggcccaggg ccgcatcgac ggcgacaacg
     1861 cggggctgcg cccgccctac aagccgcccg gcgcgggcgg catggtccag ggcatggcgc
     1921 ccgacgcgaa ggtgatcgcc atcggcaacg tctatcgcgg cggcatggcg atctacgact
     1981 cctacgcgct gatcacctac ggcctcgacg ggcggcccaa cacgggcgac gagccgcaga
     2041 tcgcttcgct cagcttcggt ttcagcggcg ggatcgactc cggctgggac ttccaatcgc
     2101 gctacctggc cgcgttgcag aaggacaatc cgcacctgag catcgtcgtc gccacgggca
     2161 acggcggccc cggctacggc accgtcacca ccccggccac cgcgcccaac gtgatcagcg
     2221 tcggcgcggc cacgcagtac ggcgagacga ccaccttcga gccgatctcg tccaccgccg
     2281 aaatcacctg gggcgacatc cagccctggt ccaaccgcgg cccgtcgatc ctgggtaacg
     2341 aagcgccctc ggtgctggcc gtgggcgcct ggggcaccgc cgacgacgcg ctcaactact
     2401 cgcgcaacgg cgccatcgcc tacaacatct ggggcggcac cagcatggcg accccgatca
     2461 cggcgggcgt gctggccctc ggctaccagg cataccacct ggccaccggc cagtggccca
     2521 ctatgaccct ggcccgcgaa ctgctggcct cgtcggcccg tgacctgaac tacggcgtct
     2581 tcagccaggg cgcgggcctg gtggatggca agcggctggt cgatctggcg gcgggccagg
     2641 cggggctgcg cgtctcgccc atgcgctgga cgccggggca gcaggcgccc gccttcgccg
     2701 ggtatgtcta ccccggcggc agcgccacca cgcccgtcac cgtcgccaac cccggcagca
     2761 cgccgctcac cgtcac
//
LOCUS       DASUYC010000013         2626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1719227, whole genome shotgun sequence.
ACCESSION   DASUYC010000013 DASUYC010000000
VERSION     DASUYC010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1719227"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2626
                     /locus_tag="VF276_00165"
     CDS             <1..>2626
                     /locus_tag="VF276_00165"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HEX6605304.1"
                     /translation="GIALFMQVFGQGLGGLRMFSMVSGTLMLVPFYPLVRQWFGVRAA
                     IIASIFLAISDVAIHFSRQEFSNITTPLFLVTGFFFLFRGLADRRLINFVLAGYAHML
                     SMYFYMGGRLTPFMLAAVAGYLFLLMPLVRLPGVYREFRQRLPDIGRLRALGRAVRRE
                     ARGVWLYANQIIVFTLACFIFASPWLFFFIDHAQTLNSRTNDKLIFNNPERMVQQYGV
                     THDPLYLGVRMPTADDVFPLPLVFEKTPVSIQLVPDGFWPQSIWHQLTTTLSILTYRA
                     DASSVYTFTGEPVAKPIEAVLIILGIAWALWRWRDTRMAVLSLWFWSTIIIGGALTID
                     APYMARIIGAVPTFAVFAAIPLSKLLAELTNVVGRFGRSVPLARTGQVLGIAALAAML
                     GFLTVKNFTDYYILYMSPYPFPEVTGQAYFVRQMNQQVTSEGRPLPRYYDLGAHLIYW
                     GHGDNRFLNHGTPGSDMVNAANDLPVIENGDRDVVFMVWDVDQQYLNTIKTYYPDGIE
                     EQFNYAPGGNGGRLFTAYRVKKEQLDARRVLLATYTPASGPAIERQEPNLGTAGPPPA
                     GLAYPVQAKWTGNLMAPTFGRYRLAIESAGPARLVIDGTPVLTTGVGTAKPEHEVILA
                     RGLHDIELTGTLASATSRITVTWQAGGSEPAPIQRKFLWSGPGRALSGEIRPMSGDPF
                     GDLPEDTPAPNSPPVTGRRVDGFLGFRDAPSALSGGGPLIGDWSGTLTVQDAGNYQFE
                     IYSNGDSTVMIDGQLVVDNRKGNGTAHAGQGQIMLTPGPHTYEVRYVWAGGTGYLEAY
                     WQPPGQPKTILGPDNLHADGGIWEPGTITEPGNAQIEVAEAPPVVAPESVIEVRSEVK
                     APRGLAVDKDGNIYVADTE"
BASE COUNT          432 a          935 c          821 g          438 t
ORIGIN      
        1 gggcatcgcc ctgttcatgc aggtgttcgg ccagggcctg ggcgggctgc gcatgttctc
       61 gatggtgtcc ggcacgctga tgctggtgcc cttctacccg ctggtgcgcc agtggttcgg
      121 cgtgcgcgcg gcgatcatcg cgagcatctt cctggccatc tccgacgtgg caatccactt
      181 cagccgccag gaattcagca acatcaccac gccgctcttc ctggtcacgg ggttcttctt
      241 cctcttccgc ggcctggccg accggcggct gatcaacttc gtgctggccg gctacgccca
      301 tatgctcagc atgtacttct acatgggcgg gcggctcacg cccttcatgc tggccgccgt
      361 cgccggctac ctgttcctgt tgatgccgct ggtgcgcctg ccgggcgtct accgcgagtt
      421 ccggcagcgg ttgccggaca tcgggcggct gcgcgcgctg ggccgggccg tgcgccgcga
      481 agcccgcggc gtgtggctct acgccaacca gatcatcgtc ttcaccctcg cctgcttcat
      541 cttcgcgtcg ccctggctgt tctttttcat cgaccacgcg cagacattga actcgcgcac
      601 gaacgacaag ctgatcttca acaatcccga gcgcatggtc cagcagtatg gggtgacgca
      661 cgatccactc tatctgggcg tgcgcatgcc gaccgccgac gatgtcttcc cgctgccgct
      721 ggtgttcgag aagacgccgg tcagcatcca gcttgtgccc gacggcttct ggccgcagtc
      781 gatatggcac caactgacga ccacgctgtc gatcctgacc taccgcgccg acgccagttc
      841 ggtctacacc ttcaccggag agccggtcgc caagcccatc gaggccgtgc tgattatcct
      901 gggtatcgcc tgggccttgt ggcgctggcg cgacacgcgc atggccgtgc tctcgctctg
      961 gttctggtcc accatcatta tcggcggcgc gctgaccatc gacgcgccgt atatggctcg
     1021 catcatcggc gcggtgccca cgttcgccgt cttcgccgcg atccccctca gcaagctgct
     1081 ggccgaactg accaacgtcg taggccggtt cgggcgctcc gtgcccctgg ctcgtaccgg
     1141 gcaggtgctg ggcatcgctg cgctggccgc gatgctcggc ttcctgacgg tcaagaactt
     1201 caccgactac tatatcctct acatgagtcc ttatccgttc ccggaagtaa ccggccaggc
     1261 ctacttcgtg cgccagatga accagcaagt gaccagcgag gggcgccccc tgcctcgcta
     1321 ctacgacctg ggcgcgcatc tgatctactg gggtcacggc gacaaccgct tcctgaacca
     1381 cggcacaccg ggcagcgaca tggtcaacgc ggccaacgat ctgcccgtga tcgagaacgg
     1441 cgaccgcgat gtcgtcttca tggtctggga cgtggaccag caatacctga acacgatcaa
     1501 gacctactat cccgacggca tcgaggagca attcaactac gcgccgggcg gcaacggcgg
     1561 gcgcctgttc acggcgtacc gcgtgaagaa ggaacaactc gacgcgcggc gcgtgttgct
     1621 ggcgacgtat acgcctgcga gcggtccggc catcgagcgg caggaaccca acctgggtac
     1681 cgccggcccg ccccccgcgg gcctcgccta ccctgtgcag gccaaatgga ccggcaacct
     1741 catggcgccc actttcggcc gctaccgcct cgccatcgaa agcgccggcc cggcccgcct
     1801 cgtcatcgac ggtacgcccg tgctgaccac cggcgtgggc accgccaagc cggagcacga
     1861 ggtgatcctg gcgcgcggac tgcatgacat cgagctaacc ggcaccctgg cctcggcgac
     1921 cagccgcatc accgtgacgt ggcaggcggg cggttcggag cccgctccca tccagcgcaa
     1981 gttcctctgg tcgggaccgg ggcgcgccct gagcggcgag atccggccca tgagcggtga
     2041 ccccttcggc gatctgccgg aagacacccc ggcgcccaac agcccgccgg tgacgggccg
     2101 gcgcgtggac ggcttcctgg gcttccgcga cgcccccagc gccctgagcg gcggcggccc
     2161 gctgatcggg gactggagcg gcacgctgac ggtgcaggat gcgggcaact accagttcga
     2221 aatctactcg aacggcgaca gcaccgtgat gatcgacggg cagttggtgg tggacaaccg
     2281 caagggcaac ggcaccgcgc acgccgggca aggccagatc atgctcaccc ccggcccgca
     2341 cacctacgag gtgcgctatg tctgggcggg cggcaccggc tatctggaag cctactggca
     2401 accacccggc cagccgaaga cgatcctggg ccccgacaac ctgcacgcgg atggcggcat
     2461 ctgggagccg ggcaccatca ccgagccggg caacgcgcaa atcgaggtcg ccgaagcccc
     2521 gcccgtagtc gcaccggaaa gcgtgatcga ggtccgcagc gaagtcaagg cgccgcgcgg
     2581 cctggccgtg gacaaagacg gcaacatcta cgtcgccgac accgag
//
LOCUS       DASUYC010000014         5280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1497376, whole genome shotgun sequence.
ACCESSION   DASUYC010000014 DASUYC010000000
VERSION     DASUYC010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5280
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1497376"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2342
                     /locus_tag="VF276_00170"
     CDS             <1..2342
                     /locus_tag="VF276_00170"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEX6605305.1"
                     /translation="AGDLQVRVAAGDGSWEAHVPLRVGQTRYETTVTVDHPRLWNTDD
                     PFLYNVAVSLPATGGDELGTRFGFREIRTDKGQLLLNGAPLFLLCALDQDLYPETIYT
                     VPSEDYLRDEFAKAKTLGLNSLRCHIKPPDPRYLDLADEMGLLIWAEIPSWRTFYPKG
                     TAQPNLLNLDDIIKRRVEATLEGMVRRDFNHPSLIIWTIVNEDWGTALPLSADDRRWV
                     REMYQRCKALDPTRLVVDNSACPHGWGPNIHVQTDLDDFHIYANIPDQARAWEHSLDQ
                     FNLRPLWTFSSHGDAERTGQEPLILSEFGNWGLPSLQKLREGAGGHDPAWFSIGPWWS
                     PWEGEAGWPRGAEDRFHRLGLESIWGDYEGFATATQWHQFAAMKFEIETMRRQPHLAG
                     YVITEFTDAYWESNGLLDFDRNPKVYYDRFATINALDMIVPQAASYACWDDHELAVTF
                     HVAHYSAAEWADARLTWTVQGTDIAGELAVPNLGRGVAQKLETRSLAIPQVEQSRMVQ
                     FRFSLQAGGQELARNTLDVLVLPAAGRAPGYGQPVAVVAHTMTGGLGRETLPEPDTAL
                     RTAATEPTEAIPQGTLEDETAATEAPVLGLQATLNALGYRTTRRLSAKVKLAVTDYPT
                     AELLDWVRAGGDLLFINNQGPSPFFWAQGRGGAYSGSWISSFSWMRPEIYKRFAVVNP
                     LSMPYQRVMPTRTILGLPVGDATFHGDLLAGMVCGWVRHPSVHTVQFRYGKGRVIATT
                     FALEEALALYNDPVAVALLHDLIDHLHSDACRPVLAANY"
     gene            2616..4595
                     /gene="acs"
                     /locus_tag="VF276_00175"
     CDS             2616..4595
                     /gene="acs"
                     /locus_tag="VF276_00175"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX6605306.1"
                     /translation="MTSTAEPSRPVPNFRRPDFEQTDRVLHEERMFTPSQEMVENANI
                     TAYIRAKGFQSYEELYAWSIANPEDYWAEQARELHWFKPWDVTFQWTEKPFFNWFAGG
                     KFNICYNCLDRHKGTPVWDRIAYYWEGENPEETSTITYADLYRGTNRFAQVLLNAGVK
                     RGDTVTIYMSRCPEMVMAMLAVARIGAMHSVIFGGFASHAIVDRVEDADAHTIITIDG
                     YRYNGKQLNLKAIVDEATDKLPDVNRVIVVRRSGMDVNMKEGRDFYYDELLKDVPEDA
                     EVPCAEMDAEDPLYVLYTSGSTGKPKGVIHVHGGYAVGCYATTKFVFDIKPDDIYWCT
                     ADPGWVTGHSYIVYGPLMTGVSSIFYEGGTMIPDAARWWNLVEKYKVTILYTAPTAIR
                     GLMRHGDQYPARHDLSSLRLIGTVGEPINPEAWLWYRHVTGDRLPVMDTWWMTETGAI
                     LISPTPITPLKPGSCTHPLPAIEADIVTLEGKPVDVNHGGFLVIRKPWPSMMRTIYKD
                     PERYRTYWTTIEGNYFAGDAAHKDADGYFWVQGRVDDVIKVSGHRLGSMEIESSLVSH
                     PAVAEAAAIGLPDELTGEHIRVYVILKSGNEQSDALQTELKQHVRRELGGIAVPKEIE
                     FVPSLPKTRSGKIMRRYIRAKAMGQDPGDLTTLES"
     gene            complement(4861..>5280)
                     /locus_tag="VF276_00180"
     CDS             complement(4861..>5280)
                     /locus_tag="VF276_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002616000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HEX6605307.1"
                     /translation="MLDDRTVSAADPHPYAGEFLWTADANRFGWTALLGQAPGSDGVP
                     AYAAAARAERLAGLPPTYICVGALDLLLEENLEYARRLLREGVPTELHVYPGAYHGFM
                     QVPNAQVSRAATRDYMAALRRALYPAENPATGSAGPA"
BASE COUNT          958 a         1835 c         1669 g          818 t
ORIGIN      
        1 ccgccggtga cttgcaggtc cgggtggccg cgggcgacgg gtcctgggaa gcgcacgtgc
       61 cgctcagggt cgggcaaacg cgctacgaaa cgaccgtcac cgtagaccat ccgcgactct
      121 ggaatacgga cgatcctttc ctctataacg tggcggtgag cctgcccgcg acgggcggcg
      181 atgaactggg cacgcgcttc ggcttccgcg aaatccgcac cgacaagggg caattgctgc
      241 tgaacggtgc gccgctgttc ctgctctgcg cgctcgacca ggatctctac ccggaaacga
      301 tctacacggt gccctcggaa gactacctgc gcgacgagtt cgccaaagcc aaaacgctcg
      361 gcctcaacag cctgcgctgc cacatcaagc cgcccgaccc gcgctacctc gacctggccg
      421 acgagatggg tctgctcatc tgggccgaga ttccctcctg gcgcaccttc tatcccaagg
      481 gcacggccca gcccaacctg ctgaacctgg acgacatcat caaacggcgg gtcgaagcca
      541 ctctggaagg gatggtgcgg cgcgacttca accatccctc gctcatcatc tggaccatcg
      601 tcaacgagga ctggggcacg gccctgcccc tgagcgccga cgatcgccgc tgggtgcgcg
      661 agatgtacca gcgctgcaag gcgctcgatc cgacccggct ggtggtcgat aactcggcct
      721 gcccgcacgg ctggggcccg aacatccacg tgcagaccga tctcgacgac ttccacatct
      781 acgccaacat ccccgaccag gcgcgcgcct gggagcactc gctggaccag ttcaacctgc
      841 gcccgctctg gaccttctcc agccatggtg acgccgagcg caccgggcag gagccgctga
      901 tcctctccga gttcggcaac tggggcctgc cctcgctcca aaagctgcgg gaaggcgcag
      961 gcggccacga cccggcctgg ttcagcatcg gcccctggtg gtcgccgtgg gagggcgagg
     1021 ccggctggcc gcgcggcgcc gaggatcgct tccaccggct cggactcgaa agcatctggg
     1081 gcgattacga gggctttgcc accgccaccc agtggcacca gtttgccgcc atgaagttcg
     1141 agatcgagac gatgcgccgg cagccgcacc tggccggcta cgtgatcacc gagtttaccg
     1201 acgcctactg ggaaagcaac ggcctgctcg acttcgaccg caaccccaag gtctactatg
     1261 atcgcttcgc gacgatcaac gcgctcgata tgatcgtgcc gcaggcggcg tcctatgcct
     1321 gctgggacga ccacgagctg gcggtgacgt tccacgtcgc gcactacagc gcggcggagt
     1381 gggccgatgc ccggctcacc tggaccgtcc agggcacgga catcgccggc gagttggccg
     1441 tgcccaacct gggccgcggg gtggcgcaga agctggagac gcgcagcctg gccatcccgc
     1501 aggtcgagca gagccggatg gtgcagttcc ggttcagctt gcaggcgggc gggcaggaac
     1561 tggcccgcaa cacgctggat gtgctggtgc tgcctgcggc gggccgcgcg ccgggatacg
     1621 ggcagccggt ggccgttgtc gcccacacca tgaccggcgg cctggggcgg gaaacactgc
     1681 ccgaacccga cacggcgctg cgcacggctg cgaccgagcc gaccgaggcc attccgcagg
     1741 gtacactgga ggacgagacc gccgccaccg aggcgcccgt gctgggcctg caagccaccc
     1801 tgaacgcgct cggctatcgc accacccgcc gcctcagcgc gaaggtcaag ctcgccgtga
     1861 ccgattatcc caccgcggag ttgctggact gggtgcgcgc gggcggcgat ctgctgttca
     1921 tcaacaacca gggccctagc cccttcttct gggcgcaggg ccgcggcggc gcctacagcg
     1981 ggagctggat cagcagcttt agctggatgc gcccggagat ctacaagcgg ttcgcggtgg
     2041 tcaacccgct cagcatgccc tatcagcggg tgatgccgac ccggacgatc ctggggctgc
     2101 ctgtcggcga cgccacgttc cacggcgacc tgctggcggg catggtctgc ggctgggtgc
     2161 gccacccctc ggtccacacc gtgcagttcc gctacggcaa gggccgggtt attgccacca
     2221 cctttgccct ggaggaggcc ctggcgctct acaacgaccc ggtggccgtg gccctgctgc
     2281 acgacctgat cgaccacctc cactccgacg cctgccgccc ggtgctcgcg gccaactact
     2341 agacaccctg gcggggagca acctaacccc ccttcccccc ttcccgccgc gggaaggggg
     2401 ggaatcgcgt ccgggaggga aagtaattcc cctccctgcg gcggggaggg gcgggggaga
     2461 ggttagctgc gccgcacgct tgccgcacac atagggcggg agtatgctat aataagcgca
     2521 ccaggtccgg ggaatacccc aggcaagcga aaaccggttt cgcaggcgcg gttccctagc
     2581 gccgtccgcg aagcacgcac ctcaaggagg ctcgcatgac cagtacagca gaaccgtccc
     2641 gccccgtccc caatttccgc cgccccgact ttgaacagac cgaccgcgtc ctgcacgaag
     2701 agcgcatgtt cacgccgtcg caggagatgg tcgagaacgc gaatatcacg gcctatattc
     2761 gcgccaaggg gttccagtcg tacgaagagc tttacgcctg gtccatcgcc aacccggaag
     2821 actattgggc tgagcaggcc agggaactgc actggttcaa gccctgggat gtcaccttcc
     2881 agtggaccga gaagccgttt ttcaactggt tcgccggggg caagttcaac atctgctata
     2941 actgcctcga ccggcacaag ggcacgcccg tctgggaccg catcgcctac tactgggagg
     3001 gggagaaccc cgaggaaacc tcgaccatca cctatgccga cctctaccgt ggcaccaacc
     3061 gcttcgccca ggtcttgctg aacgccgggg tgaaacgggg cgacaccgtg actatttaca
     3121 tgtcgcgctg cccggagatg gtcatggcga tgctggccgt ggcccgcatc ggcgcgatgc
     3181 actcggtgat tttcggcggc ttcgcctccc atgccatcgt ggaccgcgtg gaagacgccg
     3241 acgcccacac catcatcacc atcgacggct accggtacaa cggcaagcaa cttaacctca
     3301 aggccatcgt cgacgaagcc accgacaaac tgccggacgt gaaccgcgtc atcgtggtgc
     3361 gccgcagcgg catggacgtg aacatgaagg aaggccgcga cttctactac gacgagttgc
     3421 tgaaggacgt gcccgaagac gctgaggtgc cctgcgccga gatggacgcc gaggatccgc
     3481 tctacgtcct ctacacctcc ggctcgaccg gcaagccgaa gggcgtcatc cacgtccacg
     3541 gcggctatgc cgtcggctgc tatgcgacca ccaagttcgt gttcgacatc aagcccgacg
     3601 acatttattg gtgcaccgcc gaccccggct gggtgacggg ccatagctac atcgtctacg
     3661 gcccgctcat gaccggcgtc agcagtatct tctacgaggg cggcacgatg atccccgacg
     3721 ccgcccgctg gtggaacctg gtcgagaagt acaaggtcac gatcctctac accgcgccga
     3781 cggccatccg cggcctcatg cgccacggcg accagtaccc ggcgcgccat gacctgtcca
     3841 gcctgcgcct catcggcacg gtgggcgagc cgatcaaccc cgaagcctgg ctctggtatc
     3901 gccatgtcac cggcgaccgc ctgcccgtca tggacacctg gtggatgacc gagaccggcg
     3961 cgatcctgat ctcgccgacg ccgatcacgc cgctcaagcc cggctcgtgt acccacccgc
     4021 tgccggccat cgaagccgac atcgtcaccc tggaaggcaa gccggtggac gtgaaccacg
     4081 gcggcttcct ggtgatccgc aagccctggc cgtcgatgat gcgcaccatc tacaaagacc
     4141 cggagcgcta ccgcacctac tggacgacca tcgagggcaa ctatttcgcc ggggatgccg
     4201 cgcacaaaga cgccgacggc tatttctggg tgcagggccg cgtggacgac gtgatcaagg
     4261 tctccggcca ccggctaggg tcgatggaga tcgagtcgag cctggtcagc cacccggcgg
     4321 tggccgaagc cgcggccatc ggcctgccgg acgaactgac cggcgagcat atccgcgtct
     4381 atgtcatcct caagagcggc aacgagcaga gcgacgcgtt gcagaccgaa ctgaagcagc
     4441 acgtgcggcg cgagttgggc ggtatcgccg tgcccaagga gatcgagttc gtgccgtcgc
     4501 tgcccaagac gcgcagcggc aagatcatgc gccgctacat ccgcgccaaa gccatgggcc
     4561 aggaccccgg cgacctgacc acgctggagt cgtaacccga cccggcgcgc aacccggcaa
     4621 caaaaagccc ccggcgcagg ccgggggctt tttcgcgcta tcgccgatgt tgtcattctg
     4681 agcgcagcga agaatctccg tctcgtagcc caaccaccga gacccttcac tgcgctccct
     4741 agactagcac ctagtacagc gcggcggaaa taacccgccg cttgcgggac gtagacgata
     4801 ggctggtgtc cctccgtcta tcgtccgtca tcccgcgtct aacaggtgca ggcttgcttc
     4861 ttaggcaggg ccggcgcttc ccgtggccgg gttttccgcc ggatacagcg cgcggcgcaa
     4921 ggcggccatg tagtcgcgcg tggcggctcg cgacacctgc gcgttcggca cctgcatgaa
     4981 gccgtggtac gcgccggggt agacatggag ttctgtcggc acgccctccc gcagcaaccg
     5041 gcgagcgtac tccaggtttt cctccagaag gaggtctagg gcgccgacgc agatgtaggt
     5101 cggcggcagg cccgctagcc gttcggcgcg agcggccgca gcataggccg gcacgccgtc
     5161 gctgcccgga gcctgcccca gcagcgcggt ccagccgaag cggttggcgt ccgccgtcca
     5221 gagaaactct ccggcatacg gatgcggatc ggccgcgctc acggtacggt cgtcgagcat
//
LOCUS       DASUYC010000015         2910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1948554, whole genome shotgun sequence.
ACCESSION   DASUYC010000015 DASUYC010000000
VERSION     DASUYC010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1948554"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..279)
                     /locus_tag="VF276_00185"
     CDS             complement(16..279)
                     /locus_tag="VF276_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002777515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEX6605308.1"
                     /translation="MDYEWDSDKAHSNIDKHHVDFADAVQVFSDPLALTILDDYPGEE
                     RFVTLGMDGLGRILIVVYTWRGERIRIISARKATRQERKQYEG"
     gene            complement(720..1391)
                     /locus_tag="VF276_00190"
     CDS             complement(720..1391)
                     /locus_tag="VF276_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013914889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEX6605309.1"
                     /translation="MKLLLTSAGIKNPRIHSALLDLLGKPIAESSALCIPTASYGHPM
                     AGPVRAWKFISGQEPETPMCELGWKSLGVLELTALPSIEEKYWVPLVRETDVLLVNGG
                     DPLYLCYWMRQSGLAALLPALRETVYVGLSAGSMVMAPRIGEDFVGWKPPTGGDETLG
                     LVDFALFPHLDHPDLPENTMADAERWAAGLTVPRYAIDDETAIKVVDGTVEVVSEGHW
                     KRFTP"
     gene            1805..>2910
                     /locus_tag="VF276_00195"
     CDS             1805..>2910
                     /locus_tag="VF276_00195"
                     /inference="COORDINATES: protein motif:HMM:NF013056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEX6605310.1"
                     /translation="MARTATSPAPPASTSMLERRFRLRERGTSPRIEFIAGLTTFFTM
                     AYIIFVNPDIMKVTGMDPVALTIGTIIAAAVPTLVLGLWADLPWALAPGMGYNALFAY
                     TVVLGYHVPWQAALALVFLDGIAFTLIVSGPWARAIIEGIPLNLKLAAGAGIGLFIAF
                     IGMVNSGVVQLSVSAELKPGVLPFGGSSALPVLGPLSSPSVVIALIGLIITGWLMARN
                     VRAAILLGIVATTLLAWLAGAVSADWRAALAVHYPAGLGDIVQLPDLARWINLGVFKM
                     DFAGLSHFTTGALILFFLTFLVTDMMDSFGTFSGLAAKLGILDRNSNFPGSRQTMLVD
                     AGAGAFGPLVGTATVATFIESSAGVGEGGRTGLAS"
BASE COUNT          492 a         1010 c          873 g          535 t
ORIGIN      
        1 ttctcgtttc atcgtttagc cctcatactg cttgcgttcc tgccgagttg ctttgcgcgc
       61 agagataatc cgtatccgtt ccccgcgcca ggtatagact acgataagaa ttcggcccag
      121 cccatccatg cccagcgtga caaaccgttc ttcccccgga taatcgtcta gaatggtaag
      181 agccaaagga tcactaaaga cctggacggc atcggcgaaa tcgacatggt gtttgtcgat
      241 attgctgtgt gctttgtccg aatcccattc gtaatccata cacgaattat atgcacagct
      301 gtacacaagt gcaagagcta ggggaggccg ctagaggcga aatcattgcc ggcacgccac
      361 cacccaggcg aggcataggc tctgctaata agaatccgga tacccgccgc gccctggccg
      421 ggacgcagcg gatcacggac catgtttagc aggagaattg gcgcatagca cgcatcgcaa
      481 caccccccgc tcccgcgccg cccgcgggcg cccccgcgct ggagacgcgc ttccggctgc
      541 acgagcgcgg cacctccccg cgcatcgcgt ttatcgccgg gctgacgacc ttcttcacta
      601 tggcctacat catctttccc gctaacaaga tgcgcacccc gtgatgcacg atgtcgatcc
      661 ggtgccgcgg gggcaggcgg ctagcacggg cggcctggcg cggggcgagt gctgaggact
      721 tagggcgtaa accgtttcca atggccttcg gagacgactt cgacggtccc gtcgaccact
      781 ttgatggcgg tctcatcatc aatcgcgtac cgtggcaccg tcaggccggc ggcccagcgt
      841 tcggcgtcgg ccatggtatt ctccggtagg tccgggtgat ccaggtgcgg aaacagcgca
      901 aaatccacca ggcccagcgt ctcgtcgccc ccggtgggcg gcttccagcc gacgaagtcc
      961 tccccgatgc ggggggccat caccatactg ccggcgctca gccccacata gaccgtctcg
     1021 cgcagcgccg gcaggagggc ggccagtccg gactgccgca tccagtagca caggtacagg
     1081 gggtcgccgc cattcaccag caggacgtcc gtctcccgaa ccagggggac ccaatacttt
     1141 tcttcgatgc tgggcagcgc ggtgagctcc agcaccccca gggacttcca accgagctca
     1201 cacatgggcg tttcgggctc ttgtccgctg atgaacttcc aggcccggac aggaccggcc
     1261 atggggtgcc cgtaggacgc ggtgggaatg cagagggcgc tggactcggc aatcggtttg
     1321 cccaagaggt caagcagcgc gctatggatt ctcgggttct tgatgccggc ggaagtgagc
     1381 agcaatttca tgattcctct ccttaccgat ttaaggggag ccgcaaggct cctccagttc
     1441 ctctcttgga tactgaagca gagccacccc ggaacgtcgc gattgcgttt ctaccaacgg
     1501 gagtcgtatg ccctccaagg ggtcttgaaa caaagctctt gcgcttccta acaccactta
     1561 cacgtatctt gatgtagttg gcgaatgcgg aatccgcgcg ggtgccggcg aactttccag
     1621 cgccggtcag gcacgctacc gggattcgcc ccaacccatc catgcccagg gtgacgaacc
     1681 gctcttctga aaccgcagcc cccggcccgc ggcatatgct ctgctgatac gaccccggat
     1741 agcccgtgcg ctacggccat ggcgaggcgg gccaccgacc ctggtctagc aggagagttg
     1801 gcgcatggcc cgcaccgcga cctcacccgc cccgcccgcg agcacatcca tgctggagag
     1861 gcgcttccgc ctgcgcgagc gcggcacctc cccgcgcatc gagttcatcg ccgggctgac
     1921 gaccttcttc accatggcgt atatcatctt tgtcaatccc gacatcatga aggtgacggg
     1981 catggacccg gtcgcgctga ctatcggcac gatcatcgcc gccgccgtgc cgacgctggt
     2041 gctggggttg tgggccgacc tgccctgggc gctggcgccc ggcatgggct acaacgccct
     2101 cttcgcctac accgtcgtgc tgggctacca tgtgccctgg caggccgcgc tggccctcgt
     2161 cttcctcgac ggcatcgcct tcaccctgat cgtctccggc ccctgggcac gcgccatcat
     2221 cgagggcatc ccgctcaacc tgaagctggc cgcgggcgcg ggcatcggcc tgttcatcgc
     2281 cttcatcggc atggtcaatt cgggcgtcgt ccaactcagc gtcagcgccg aactcaagcc
     2341 cggcgtgctg cccttcggcg gcagcagcgc gctgccggtg ctcggcccgc tcagcagccc
     2401 cagcgtggtc atcgcgctga tcggcctgat catcaccggc tggctcatgg cgcgcaacgt
     2461 gcgcgcggcc atcctgctgg gcatcgtggc gaccaccctg ctggcctggc tcgccggtgc
     2521 tgtctccgcc gattggcgcg cggccctggc cgtccattat cccgccggcc tgggcgacat
     2581 cgtgcaactg cccgacctgg cccgctggat caacctgggc gtgttcaaga tggacttcgc
     2641 cgggctgagc cacttcacca ccggcgcgct gatcctcttc ttcctcacct tcctggtgac
     2701 ggacatgatg gacagctttg gcaccttcag cggcctggcc gccaagctgg gcatcctcga
     2761 ccgcaacagc aacttccccg gctcgcgcca gaccatgctc gtcgacgcgg gcgcgggcgc
     2821 cttcggcccg ctcgtgggca cggccaccgt cgccaccttc atcgagagca gcgcgggcgt
     2881 gggcgagggc gggcggaccg ggctggcctc
//
LOCUS       DASUYC010000016         2092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1100089, whole genome shotgun sequence.
ACCESSION   DASUYC010000016 DASUYC010000000
VERSION     DASUYC010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100089"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="VF276_00200"
     CDS             complement(<1..427)
                     /locus_tag="VF276_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605311.1"
                     /translation="MDKAKPPAEDGTLSGVDPAQALAELRDQIRQRSARLGGRLDAGA
                     PGGGGGADFSMNQVRQSVDEVNDLWFVSAHLPITWKVPGVGTAIAYAKKATRVLLRWY
                     VNPIVEQQNRYNSAVARALVEITAYQERLTREWQVLEERV"
     gene            complement(441..935)
                     /locus_tag="VF276_00205"
     CDS             complement(441..935)
                     /locus_tag="VF276_00205"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX6605312.1"
                     /translation="MVTDREIAELLARLAAVPARFATACGRFEEADAVVHPAAGEWSP
                     AEVLAHLRASHDITEPRILAILARDNPPLPAFDERRWQEVARYVALPMTESLETLRLR
                     RHELVRALRGLPAAAWMRIGLHEERGPLTVLEIARDIAAHDEEHCIQVERMTLDGGAQ
                     AGSV"
     gene            complement(964..>2092)
                     /locus_tag="VF276_00210"
     CDS             complement(964..>2092)
                     /locus_tag="VF276_00210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX6605313.1"
                     /translation="PQVRVLAPGANLGFGGGANLGWQTATGDILAVVNPDVRVRSDWL
                     RALAAGLHTHADARAAIAGGKLVYPDGRVQHAGGAFHFPAATTEHIGRGATDSPAFAQ
                     DREMAYVTGGALAITRAAYAALGGFDAGFWPVYFEDVDLCTRAWDAGYSVWYIAAAVA
                     THAESASLAHGGPDYFRYYHRNRLRYVLRHYARPQVLGAFGPAEEARLRGDLELADRS
                     ASLNLYADPAALSDVAGTAVPPPLPSAAPGPATARRDRLAGMVGEALEHWRVTPPPFR
                     SRVPGLAWLRTRLNNLWTRWYVDPIVAQQVEYNAALARTVRELADQVAGLEAALLVRT
                     GLEQATTDQLLPPTPTSSAPAGAGDNQPLSTGMDHGSRGDA"
BASE COUNT          279 a          759 c          750 g          304 t
ORIGIN      
        1 gcacgcgctc ttccagcacc tgccattcgc gagtcagccg ctcctggtag gcggtgatct
       61 cgaccagggc gcgggccacg gcgctgttgt agcggttctg ctgctcgaca atcgggttga
      121 cgtaccagcg cagcagcacg cgcgtcgcct tcttggcgta ggcgatggcc gtgcccacgc
      181 ccggcacctt ccaggtgatc ggcaggtggg cgctgacgaa ccagaggtcg ttgacctcgt
      241 ccacgctctg gcgcacctgg ttcatgctga aatcggcgcc gcccccgccg cccggcgctc
      301 cagcatccag gcgtccgccc aggcgcgcgc tgcgctgccg gatctggtcg cgcaattcgg
      361 ccagggcctg cgcggggtcc acgccggaga gggtgccgtc ttctgctggt ggttttgctt
      421 tgtccattga tagtcctgtc ctacacgctt ccagcctggg ctccgccgtc cagggtcatg
      481 cgctcgacct ggatgcaatg ctcctcgtcg tgggcggcga tgtcccgcgc gatctcgagc
      541 acagtgagcg ggccgcgctc ctcgtgcagc ccgatgcgca tccaggcagc ggcgggcagc
      601 ccgcgcagcg cccgcaccag ttcgtgccgc cgcaagcgca gcgtctccaa tgattcggtc
      661 atgggcagtg cgacgtagcg ggccacctcc tgccagcggc gctcgtcgaa ggcgggcagc
      721 ggcgggttgt cgcgggcgag gatcgccagg atgcgcggct cggtgatgtc gtgcgaggcg
      781 cgcagatggg ccagcacctc cgccggcgac cattcgcccg ccgccggatg caccaccgcg
      841 tcggcctcct cgaaccggcc acaggccgtg gcgaagcgcg cgggcaccgc cgccaacctg
      901 gccagcagtt ccgcgatttc gcgatccgtt accatgcctg ctcctttcgc ggccaaccgg
      961 gcactacgca tcgccccgcg atccgtggtc cattccggtg ctgagcggtt ggttgtctcc
     1021 tgccccggcg ggcgccgagg acgtgggcgt cgggggcagc agttggtccg tggtcgcctg
     1081 ttccagcccc gtgcgaacca gcagtgcggc ttccaggccc gcgacctgat cggccagttc
     1141 gcgcaccgtg cgggccagcg cggcgttgta ctcgacctgt tgggccacga tgggatcaac
     1201 gtaccagcgc gtccagaggt tattgagccg ggtgcgtagc caagccagcc ccggcacccg
     1261 cgaccggaac ggcggcggcg tcacccgcca gtgctccagc gcctccccta ccataccggc
     1321 aagccggtcg cggcgggccg tggccgggcc gggagcggcg gagggcagcg gcggcggcac
     1381 agcggtcccg gccacgtcgc tcagcgccgc cgggtcggcg tagaggttca ggctggcgga
     1441 gcggtcggcg agctccaggt cgccgcgcag gcgggcctcc tccgcggggc cgaacgcgcc
     1501 cagcacctgg ggccgggcgt agtggcgcag cacgtagcgc agccggttgc ggtggtagta
     1561 gcggaagtag tcggggccgc cgtgggccag cgaggccgac tcggcgtgag tggccaccgc
     1621 cgcagcgatg taccagaccg agtacccggc gtcccaggcg cgggtgcaga ggtccacgtc
     1681 ctcgaagtag accggccaga accccgcgtc gaagccgccc agcgccgcgt aggccgcccg
     1741 cgtgatcgcc agcgcgccgc ccgtcacata ggccatctcg cggtcctggg cgaacgccgg
     1801 gctgtccgtc gcgccgcgcc cgatatgctc ggtggtcgcg gcggggaagt ggaaggcgcc
     1861 gcccgcgtgc tgcacccgcc cgtcgggata gaccagctta ccgccggcga tagccgcccg
     1921 cgcatcggcg tgggtatgaa gacccgcggc gagggctcgc agccagtcag agcgcacgcg
     1981 cacatccgga ttgaccacgg ccaggatgtc gccggtggcc gtctgccagc cgaggttcgc
     2041 cccgccgccg aagcccagat tcgcgcccgg cgccagcacg cgcacctgcg gg
//
LOCUS       DASUYC010000017         5633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1189268, whole genome shotgun sequence.
ACCESSION   DASUYC010000017 DASUYC010000000
VERSION     DASUYC010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5633
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189268"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /gene="pgl"
                     /locus_tag="VF276_00215"
     CDS             <1..726
                     /gene="pgl"
                     /locus_tag="VF276_00215"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEX6605314.1"
                     /translation="RDAADLATRAADMFAALALAAGVDWRTISVALAGGSTPRAMYEL
                     LAGDNYREGIHWPLIHFFWGDERYVPADSKDSNYRMAYDALLSKVPVPEANIHRMVTD
                     YPDENTAAAAAAQDLVDHFKLNPGEFPRFDLVLLGLGDDGHTASLFPGKPALHEQARL
                     VVPTPPGRLPPPVDRLTMTLPVLNAAAHVVFLVAGAGKAGIVGEILGSAENPEPYPAQ
                     LVCAPNGRLTWMLDEAAAAELHR"
     gene            723..1400
                     /gene="rpe"
                     /locus_tag="VF276_00220"
     CDS             723..1400
                     /gene="rpe"
                     /locus_tag="VF276_00220"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011433633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX6605315.1"
                     /translation="MTTRRTDVEICPSILSADFTQLGAQVRAVEEAGARRLQIDVMDG
                     TFVPNITMGAGIVEAVRGVTGMLVEAHLMIVRPELHIPAFAKAGADLIIVHQEAAVHL
                     HRAVQQIKGLGKRAGVALNPATPLVMVEEILEYVDLVLIMTVNPGFGGQDFIASMLPK
                     IRRLRAMLDERNLSCEIEVDGGIHTATAPLVVEAGARVLVAGSAVYSKQQSVPDAIAA
                     LRASYGG"
     gene            1837..3348
                     /locus_tag="VF276_00225"
     CDS             1837..3348
                     /locus_tag="VF276_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6605316.1"
                     /translation="MSPLIHRLQGGAGSSEDRTNETADYAIASGADGGAGGAAAMVAP
                     APAPQPMAGPASSAASAAPPIGNYLAEPKTIAASGLNPSIISDLILKTLYYTSYLNGH
                     ELAATIRLPFYGVLDQLTVVLKREELLEITGTSGLGEAGYQYLLTRKGMDRAEAALRR
                     SAYVGPAPVPLNKYIEIVLIQAQRRPRIDRQVVHNALTGMILTQDMIDKVGAAVNAGR
                     SLFLFGAPGNGKSMLAERIGKMLGGNIAVPYAIEADGQIIQFFDLNLHRPVPFQTPNS
                     GDQVGLGGIADPRTAEYSDRRWVQTQRPVVIVGGELTMESLDLIYHGDTGFYEAPFQL
                     KANNGVFLIDDFGRQQMSPQQLLNRWIVPLEKSVDFLTLHTGKKLQVPFELFLVLSTN
                     LEPADLVDEAFLRRIQNKLNMPDPTPEQFRNIFISECQRQGVPFDENGLAYMMREHYI
                     RHARPLRAVHPRDLLRQLVGLARYQTLPPQLIPHLLDQSANTYFVPMKKPDKL"
     gene            3615..3896
                     /locus_tag="VF276_00230"
     CDS             3615..3896
                     /locus_tag="VF276_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605317.1"
                     /translation="MLRPAMRILAVSCGLLLTMGGVTAAETQAAGSTPTIPPAVSTPA
                     PAPPKVTDAPGTVDPGFAVTVQGYPDPGFTATVKGYPDPGFEGRLTPAR"
     gene            4115..4843
                     /gene="rpiA"
                     /locus_tag="VF276_00235"
     CDS             4115..4843
                     /gene="rpiA"
                     /locus_tag="VF276_00235"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEX6605318.1"
                     /translation="MSTSDELKREAAAVAVQYIADGMAVGLGTGSTADFFLQALGARV
                     QAGLRVVGVPTSARTEARARELGIPVTTLDATPRLAVTVDGADEVDLATFYVVKGRGG
                     ALLREKIVAVASDLEIIIVDGGKVVPALGARGTVPVEVIPFGWHNTAAALTALGAAVE
                     LRSLAPAEAVNTSPAEHAFVTDSGNFILDCHWPAIADAAALGAQIKAITGVVEHGLFI
                     GIAGRVIVAADTGVQVYERPSSTG"
     gene            4818..5570
                     /locus_tag="VF276_00240"
     CDS             4818..5570
                     /locus_tag="VF276_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX6605319.1"
                     /translation="MSDHPAPADSSPTTDIVFLYDVDNTLLNNDVLKHDLDAQLDALL
                     GVPESDLFWRIYEEVREEEDVVDIPEALRRFEEQCTNRPLCLSIRAVFEQVNFPHYLY
                     PGALDTLRYTASLGTNVILSDGDQVFQRRKIEESGIADAAACHVLIYVHKEEHVDEIR
                     ARFPARHYVMIDDKPRILKALPDRFGGGLRAVFVCQGKYARDPKRPADLAPDLTIAGI
                     ADLQEYSAEELRTGRFHRQGSCPVDAGAAQAL"
BASE COUNT          960 a         1924 c         1781 g          968 t
ORIGIN      
        1 cgcgacgccg cggacctcgc gacgcgggcg gcggacatgt tcgccgccct ggccctcgcc
       61 gccggggtgg actggcgcac tatctcagtc gccctggccg gcggctcgac gccgcgcgcc
      121 atgtatgaat tgctggccgg cgacaactat cgtgagggca tccactggcc gctcatccac
      181 ttcttctggg gcgatgagcg ctatgtgccc gccgacagca aggacagcaa ctatcgcatg
      241 gcctatgatg ccctgctgtc gaaggtgccg gtgcccgagg cgaatatcca ccgcatggta
      301 acggactacc ccgatgaaaa cacggccgcg gcggcggcgg cacaggacct ggtggaccac
      361 ttcaagctga atccaggcga gttcccccgc ttcgacctgg tcttgctcgg cctgggcgac
      421 gacggccaca ccgcctcgct gttccccggc aaaccggcgc tgcacgagca ggcgcgcctg
      481 gtggtgccga ccccgccggg gcgcctgccg ccccccgtgg atcgcctgac catgacgctg
      541 cccgtgctca acgccgccgc gcacgtcgtc ttcctggtgg ccggggcggg caaggccggg
      601 attgtcggcg agatcctggg cagcgccgag aatcccgaac cctacccggc ccaactagtg
      661 tgcgcgccca atggccgctt gacctggatg ttggacgagg ccgccgccgc ggagttgcac
      721 cgatgaccac ccggcgcacc gatgtcgaga tctgcccgtc cattctctcc gcggacttca
      781 cccagctcgg cgcgcaggtg cgcgcggtcg aggaagcggg cgcgcgccgc ctgcagatcg
      841 acgtgatgga cggcaccttc gtgcccaaca tcaccatggg cgcgggcatt gtcgaagccg
      901 tgcgcggcgt caccggcatg ctggtcgaag cgcacctgat gatcgtgcga cccgaactgc
      961 acatcccggc ctttgccaaa gccggcgccg acctgattat cgtgcatcag gaagccgccg
     1021 tgcatctgca tcgcgccgtg caacagatca agggcctggg caagcgcgcc ggggtggcgc
     1081 tgaacccggc cacgccgctg gtcatggtcg aggagatcct ggagtacgtc gatctggtgc
     1141 tgatcatgac ggtcaacccc ggcttcggcg ggcaggactt tatcgccagt atgctgccga
     1201 agatccgccg cctgcgcgcc atgctcgacg agcgcaacct gtcgtgcgag atcgaggtcg
     1261 acggcggcat ccacaccgcg acggccccgc tggtggtcga agccggggcg cgggtgctgg
     1321 tggccggctc ggcggtctac agcaaacagc agagcgtccc cgacgccata gccgccctgc
     1381 gcgccagcta tggcggctga cgctgctctg cgcgcttgcc tatccgtgcc ctgcccggcg
     1441 taacccgccc ggcggggcac ggcgctttcg gccagccggc ggcgcacgca tgcagcgcca
     1501 cctggccctg cgggaccggg cgtccctgtc tgctaccccg ttattaccgc taacctccta
     1561 ccgcccaggg actttcgggt acgagcaccg gtttgggtga gaaatttgcg cctcatttta
     1621 ggcagaagcg ctcgggttac cggtccgggc gctgctggcg ctcgttacgg cgccagccgg
     1681 ccgctgccac aaatgattga cacccctcag gccacctgtt atactcgcaa taggttcacc
     1741 ggcctgatcg gagagttagg cgtgcacccc gccgatccgg ctcgtgggtt gttggttccc
     1801 cagtagtgta ccgccacgcg caaggagagt gacccgttga gtcccctaat tcatcgcctc
     1861 cagggagggg ccggctcgtc cgaagatcgg acgaacgaga ctgccgatta cgcaattgcc
     1921 tccggcgcgg atggcggcgc gggcggcgcg gcggccatgg tcgccccggc cccggccccc
     1981 caaccaatgg cggggccggc gtcgagtgcg gctagcgcgg ctcccccgat cggcaactac
     2041 cttgccgagc cgaagaccat cgccgccagc ggcctcaacc ccagcatcat cagcgacctg
     2101 atcctcaaga cgctctatta caccagttac ctcaacgggc atgagctggc cgccacgatc
     2161 cgcctgccgt tctacggcgt gctggatcag ctcaccgtgg tgctcaagcg cgaggaactg
     2221 ctggaaatca caggtacaag cggcctgggc gaggccggct accagtacct gctgacgcgc
     2281 aagggcatgg accgcgcgga agcggccctg cgccgctcgg cctatgtcgg cccggccccg
     2341 gtgcccttga acaagtatat cgagatcgtg ctcatccagg cccagcgccg cccccgcatc
     2401 gaccggcagg tggtgcataa cgccctcacc ggcatgatcc tcacccagga catgatcgac
     2461 aaggtaggcg cggcagtcaa cgccgggcgg tcgctcttcc tcttcggcgc gccgggcaac
     2521 ggcaagagca tgctcgccga gcgcatcggc aagatgctgg gcggcaacat cgccgtgccc
     2581 tatgccatcg aggccgacgg ccagatcatc cagtttttcg acctcaacct gcatcgcccg
     2641 gtgcccttcc agacgcccaa cagcggcgac caggtgggcc tcggcggcat cgccgacccg
     2701 cgcaccgccg agtactccga ccggcgctgg gtgcagacac agcgccccgt cgtcatcgtc
     2761 ggcggcgaac tgacgatgga gagcctggac ctgatttacc acggcgacac cgggttctac
     2821 gaagccccct tccagctcaa ggccaacaac ggcgtcttcc tgatcgatga ctttggccgc
     2881 cagcagatga gcccgcagca attgctcaac cgctggatcg tcccgctgga gaagagcgtg
     2941 gacttcctga cgctgcatac cggcaagaag ctgcaggtgc ccttcgagtt gttcctggtc
     3001 ctttcgacca acctggaacc cgccgacctg gtggacgaag ccttcttgcg ccgcatccag
     3061 aacaaactga acatgcccga cccgacgccg gagcagttcc gcaacatctt catcagcgag
     3121 tgccagcgcc agggcgtgcc cttcgacgag aacgggctgg cctacatgat gcgcgagcac
     3181 tacatccgcc acgcgcgccc gctgcgtgcc gtgcatccgc gcgacctgtt gcgccaactg
     3241 gtgggcctgg cccgctacca gaccctcccc ccgcaactga tcccgcacct gctcgaccag
     3301 tcggccaaca cctacttcgt gccgatgaag aagccggata agttgtagat cggacagcgt
     3361 agcagcgctg ctgaaccgtg ggtgcgtagc cgctatacca gcatatcatc cctccgcttc
     3421 cgtgaagggt acgaagccgc cttcgggcgg cctctgtgtt gcctgccggt atacgtctac
     3481 gaacctgtat aactcccaaa cagtatccag cctgggaacc ctgctaccca ttccagcgtc
     3541 aagaaggtat aatagaccca atacacagca gccctgctgc tgtgccggca ccagtgagga
     3601 gaaagatcac gtccatgctt cgtcctgcta tgcgtatcct ggctgttagt tgtgggctgt
     3661 tgctcacgat ggggggcgtg accgccgccg agacacaggc cgcgggatcg acccccacga
     3721 tcccgcctgc cgtgagcacg cccgcgcctg ccccgcccaa agtgaccgat gcaccgggta
     3781 ccgtggatcc tggttttgcc gtcaccgttc agggctaccc agatcccgga ttcaccgcta
     3841 cggtcaaggg ctatccggat cccggctttg aggggcggtt gaccccggcg cggtagcgct
     3901 ttcccgccgg tggagcagct ccgccggctg tttgcccgct cgaacgagcc gcctcagggc
     3961 ggcttgtttg attccctcgg gttggcatgg tcgttgctct cgtggtaggg gcggctggcc
     4021 gctgggcaaa gcccggcgtc tgcccggtgt ctgttggcga gcggtggata ataagtgcgc
     4081 gtgatgcggc acaggtttgt gctataatgt tgccatgagt acttccgatg aactgaagcg
     4141 cgaagccgcc gccgtggccg tccagtatat cgccgacggg atggcggttg gcctgggcac
     4201 gggcagcacg gcggactttt ttctccaggc gctgggtgcc cgtgtgcagg cggggctgcg
     4261 cgtggtcggc gtgcccacct cggcgcggac cgaggcgcgg gcgcgggaac tcggcatccc
     4321 cgtcaccacg ctcgacgcga ctccgcgcct ggctgtgaca gtggacggcg ccgacgaagt
     4381 ggacctggcg acgttttacg tggtgaaggg gcgcggcggc gcgttgttgc gcgagaaaat
     4441 cgtcgccgtc gccagcgacc tcgaaatcat catcgtcgat gggggcaaag tcgtgcccgc
     4501 gctgggcgcc aggggcaccg tgccggtgga ggtgattccc ttcggctggc acaataccgc
     4561 cgccgctctg acagcattgg gcgccgcggt ggagttgcgg tcgctggccc cggcggaggc
     4621 cgtcaatacg tcaccggcgg agcacgcctt tgtcaccgac agcggcaact ttatcctcga
     4681 ttgccactgg ccggcgatcg ccgatgcggc ggcgctgggt gcgcagatta aagcgatcac
     4741 gggagtcgtc gagcacggct tatttatcgg catcgcggga cgggttatcg tcgccgccga
     4801 tacaggggtg caagtctatg agcgaccatc cagcaccggc tgattcatcg ccaaccaccg
     4861 atattgtgtt tctctacgat gtcgataaca cgctgctcaa taacgacgtg ctcaagcacg
     4921 accttgacgc gcagctagac gcactcctgg gcgtgccgga aagcgacctg ttctggcgga
     4981 tttacgaaga ggtgcgcgaa gaagaggatg tagtcgacat cccggaggcg ctgcggcggt
     5041 ttgaagagca gtgtaccaat cgcccgcttt gcctatcgat acgtgccgtc tttgaacagg
     5101 taaatttccc gcactatctc tatcccggtg ccctggacac gttgcgttac accgcctcgc
     5161 tgggcaccaa tgtgattttg tcggacggcg accaggtctt ccagcgccgc aaaatcgaag
     5221 agagcggtat tgccgacgcc gctgcgtgcc atgtgctgat ctatgttcac aaggaagagc
     5281 acgtagacga aatccgggcg cgcttccctg cccgccacta cgtgatgatc gacgataagc
     5341 cgcgcatcct caaagctctg cccgatcgct ttggcggcgg cctgcgcgcg gtgtttgtct
     5401 gccagggcaa atatgcccgc gatccgaagc gccccgccga cctcgcgccc gacctgacca
     5461 tcgccggcat cgccgacctg caggagtaca gcgcagagga actgcgcacg ggccgcttcc
     5521 accgccaggg gtcgtgcccc gtggacgccg gggccgccca ggcgctctaa cacgtaccgc
     5581 tagacagtct ccccgcaaag cgctcccgcc cggccatcat tctgccggtg agc
//
LOCUS       DASUYC010000018         3028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_792493,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000018 DASUYC010000000
VERSION     DASUYC010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792493"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VF276_00245"
     CDS             <1..414
                     /locus_tag="VF276_00245"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEX6605320.1"
                     /translation="MGSAQQINLMKLLLREADREPDLIATLSQTISQRALTPMGAYMR
                     RQMDLGRIRRDNPEFVAQAFLGMFIAVVLRREILQEPESRAWNLDEYVDTAVGVFLRG
                     IIPAPGEVLEPAPAPPPVPAVDHAARKVDHIPIEE"
     gene            424..1227
                     /locus_tag="VF276_00250"
     CDS             424..1227
                     /locus_tag="VF276_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6605321.1"
                     /translation="MSSSKADHQSVVEVAGLVKRFDGVRALDEMHLTVPAGMIYGLLG
                     PNGAGKTTLIRALLGLVRPDAGTVRVLGHPIPAQAAAVRANIGYMTQAPALYSDLSVR
                     ENLEFFARVFDMNDTARRKARIQEVLELVDLADRQTSPVANLSGGMRQRVSLACALVH
                     RPRLLLLDEPTVGVDPQLRHAFWTYFRTLAASGVTLVVSSHVMDEAERCDRLGLMRAG
                     KLLAEGTAASLRAQTGQATLEDAFLALAGVLPIQGAEAIAEREETGVVG"
     gene            1246..2397
                     /locus_tag="VF276_00255"
     CDS             1246..2397
                     /locus_tag="VF276_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6605322.1"
                     /translation="MSPVHVRALATRIVQQLRRDHRTLALLFVAPIIILALLGYLLRS
                     QESGTLVVGISNQDQPVAPLNVSVAGRLIDTLRQNDRLTVRDLSGDTTAVRDAVRDGT
                     VDAALMFGPTFTADLLARKPVALDVILEGSNPAKTSSAVPALQGALLQAMTSLSAALP
                     GASPAASLPQLRLQPDLLYGSTDLKSLDYFAPVLIGFFAFFLIFLLTCVSFLRERTLG
                     TMERLAASPVTRGEVVVGYMLGFGLFALIQSAIIVLFTAYVLQVHYSGNLAWIFLVTL
                     ILAIGAVNLGIFLSSFARTELQAVQFIPLVIVPQGLLSGLLWPVKAMPDWLQVVARAL
                     PLTYANEALTNIMIRGKGLADTALQIGVLLLFAALMVVLASLTLRREVA"
     gene            2439..>3028
                     /locus_tag="VF276_00260"
     CDS             2439..>3028
                     /locus_tag="VF276_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018771715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEX6605323.1"
                     /translation="MSISEHEPQAGSGSAAAEGVLVVPLRDLDGSSLSLAGGKAAHLG
                     ELIRGGFAVPDGFCATTAAYAQVAAAAGIDPLLAELAGLDAEDTARQAHLAGQIRAAL
                     LRTPLPGDLEATLTRAYETLGGNAAVPVAVRSSATAEDLPGASFAGQQETYLNVAGSA
                     AVLDSVRRSFASLWTDRAVSYRAARGIDPRTVRLAAV"
BASE COUNT          447 a          998 c         1079 g          504 t
ORIGIN      
        1 atgggcagcg cgcagcagat caacttgatg aagttgctgc tgcgcgaggc ggatcgcgag
       61 ccggacctga tcgcgacgct gagccagacc atcagccagc gcgcgctcac gccgatgggg
      121 gcgtacatgc gccgccagat ggatctgggg cgcatccggc gcgacaaccc ggaatttgtg
      181 gcccaggcgt tcctggggat gttcatcgcc gtcgtgttgc gccgcgagat cttgcaggaa
      241 ccggagagcc gggcctggaa ccttgacgaa tatgtggaca ccgccgtggg cgtgtttctg
      301 cggggcatca tcccggcgcc cggcgaggtc ctggaaccgg cgcccgcgcc acccccggtc
      361 ccagcggtgg accacgcggc gcggaaggtg gaccatatac cgatcgagga atgaggtagc
      421 agcatgagca gcagcaaggc ggaccaccag tcggtggtgg aggtggcggg gctggtcaag
      481 cggttcgacg gcgtgcgcgc cctcgacgag atgcacctga ccgtgcccgc gggtatgatc
      541 tatgggctgc tgggaccgaa cggcgcgggc aaaaccacgc tgatccgcgc gctgctgggc
      601 ctggtgcggc ccgacgccgg gaccgtgcgc gtcctcggcc atcccatccc ggcccaggca
      661 gcggcggtgc gtgcgaacat cggctacatg acgcaggcgc ccgcgctcta ctcggacctg
      721 agcgtgcgcg agaacctgga gttcttcgcg cgggtgttcg atatgaacga caccgcccgg
      781 cgcaaggcgc gcatccagga agtgctcgaa ctggtcgacc tggccgatcg gcagaccagc
      841 ccggtggcga acctgagcgg cggcatgcgc cagcgcgtgt cgctggcctg cgcgctggtc
      901 caccggcccc gtttgttgtt gctggacgag ccgacggtgg gcgtcgatcc gcaactgcgc
      961 cacgccttct ggacctactt ccgcacgctg gccgccagcg gcgtgaccct cgtggtatcg
     1021 agccacgtca tggacgaagc cgagcgctgc gaccggctgg ggctgatgcg ggccgggaag
     1081 ctgctggccg agggcaccgc cgcgagtttg cgcgcccaga ccggccaggc gaccctggaa
     1141 gatgccttcc tggcgctggc cggcgtcctg ccgatccagg gggcggaggc tatagcggag
     1201 cgggaagaga ccggcgtggt cgggtgagct gaaaggaagt agacgatgag tccggtccat
     1261 gtgcgtgcgc tggcgacgcg gatcgtccag caattacggc gcgatcatcg caccctggcg
     1321 ctgctgttcg tggcgccgat aatcatcctc gcgctcctgg ggtacctgct gcgctcgcaa
     1381 gaatcgggca cgctggtggt cggcattagc aaccaggatc agccggtcgc cccgctcaat
     1441 gtcagcgttg ccggtcgcct gatcgacacg ctgcggcaga acgaccgcct gacggtgcgc
     1501 gatctctccg gcgacaccac cgccgtgcgc gatgccgtgc gcgacggcac ggtggacgcg
     1561 gcgctgatgt tcggcccgac cttcacggcg gatctgctgg cgcgcaagcc ggtggcgctg
     1621 gacgtgatcc tggagggatc gaacccggcg aaaaccagta gcgctgtgcc cgcgctccag
     1681 ggcgcgctgc tccaggcgat gacgagcctg agcgcggcgc tgccgggcgc gagtcccgcg
     1741 gcgagcctgc cgcagttgcg cctgcaaccc gacctgctct acggcagcac cgatctgaaa
     1801 agcctcgact acttcgcgcc cgtgctgatc ggctttttcg ccttcttcct gatcttcctg
     1861 ctcacctgcg tctcgttcct gcgcgagcgc acgctgggca cgatggaacg gctggccgcg
     1921 tccccggtca cgcggggtga ggtcgtcgtc ggctacatgc tgggctttgg cctgttcgcg
     1981 ctcatccagt cggcaatcat cgtgctgttc accgcctacg tcttgcaggt ccactacagc
     2041 ggcaacctgg cctggatctt cctcgtgacc ctgatcctgg caatcggcgc cgtgaacctg
     2101 ggcatcttcc tgtcgagctt cgcgcggacg gaactccagg cggtgcagtt catcccgttg
     2161 gtgatcgtgc cgcaggggtt gttgagcggc ctgctctggc cggtgaaggc catgcccgac
     2221 tggttgcagg tggtggcgcg ggcgctgccg ctcacctacg ctaacgaggc gctgacgaac
     2281 atcatgatcc gcggcaaggg cctggccgac acggccttgc agatcggcgt gctgctgctg
     2341 ttcgccgcgc tgatggtggt gctggcctcg ctcaccctgc gccgggaggt ggcataagcc
     2401 gcgtgccggt gctctcggtt gtataaggga ggccgctcat gagcatatcc gaacacgaac
     2461 cgcaggcggg aagcgggtcc gccgcagcag aaggcgtgct ggtcgtgccg ctccgggatc
     2521 tcgatggcag ttcgctctcg ctcgccgggg ggaaggcggc ccacctgggg gaactgatcc
     2581 gcgggggatt cgccgtgccc gacggcttct gtgcgaccac ggcagcttat gcccaggtgg
     2641 ccgcggcggc ggggatcgat ccgctgctgg cggaactggc gggcctggat gccgaagaca
     2701 cggcgcggca ggcacacctg gccgggcaga tccgcgcggc cttgctgcgg acgcctctac
     2761 cgggcgatct ggaggccacc ttgacccgcg cctatgagac cctgggcggg aacgctgccg
     2821 tgccggtcgc cgtccgctcg tcggcaacgg cggaagatct gccgggcgcc agcttcgccg
     2881 gccagcaaga gacctatctc aacgtggctg gcagcgccgc cgtgctggat tctgtccgcc
     2941 gcagcttcgc ctcgttgtgg accgatcggg cggtcagcta tcgcgcggcg cggggcatcg
     3001 atccgcggac cgtgcgcttg gccgctgt
//
LOCUS       DASUYC010000019         4249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1872158, whole genome shotgun sequence.
ACCESSION   DASUYC010000019 DASUYC010000000
VERSION     DASUYC010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1872158"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(303..2687)
                     /locus_tag="VF276_00265"
     CDS             complement(303..2687)
                     /locus_tag="VF276_00265"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX6605324.1"
                     /translation="MAVTALPMASQAAPAQQGGTRHFPETNQDVSGRFLEVWESGRSY
                     GDSLYINGLPLTDVHDEISLTDGKIYKTQWFERARFEAHPENQKPYDVLLGLLGVFAA
                     EGRKDTPFKTVDNPGGGVQWFPETKHTLGDSSTGGKAIAKFWNQKGGLSQFGFPISQP
                     FQEVTRETDPKVAGKSFLVQYFERQRFEYHPENAGTPFEVLLGRLGAEQKGQVPVMEK
                     AVAKGNNPVDVLRIGRGQDPATLIPWDDNTLIGTNVRNFVYNGLTKRDDKENVVPDLA
                     AYVPTLDNGGAYYVGTGDDKRLVVKYKLKRGVKWFDGQPFDSNDVIFTYKLVLNPDFP
                     AAGRQPAQYFASVDNPDPYTLIFNYLTWKEAAALIARDKDTYAFLQAFVDKKIPVINP
                     TYNEVLGFILPQHALAKMAPGDIDSSDYSQAVWGTGPYHVTKFQTGQTLEMERNPNYS
                     VTPDKPSFAKVSWPLFSDNKQLPVGIATGTLDLTTSESITPDLITSIQTTVEKAGKGK
                     LLNQGGYSYEHIDFNVTKEPFNDVRVRQALAYSLDRNAINNAIFGGQLTIMNAWIPPT
                     HWASAENPDVMKKYPDLTSQIVKYDYNVAKANELLDAAGWVKGADGIRSKNGKTLKVD
                     WLTTSKAYRKLIATAQQQYLKAVGFDSTPDVQAAGQVFANPPDGPLYSGSYGNFGVIE
                     FAWQFTSDEIDPTGLFDSTQIPSAGNGFGGGNDMFWSNADADKYGRDTLTQIGRTSDR
                     IQSFLRQQIAVSKDIPTFPMFGLPSLYIATNNFQNLQAHAGGYLADPQMWYLGK"
     gene            complement(3244..4191)
                     /locus_tag="VF276_00270"
     CDS             complement(3244..4191)
                     /locus_tag="VF276_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX6605325.1"
                     /translation="MTVAELFQAIDRERRKVIVGQDATFELLIVALLAGGHVLLEGVP
                     GIAKTLMAKTLAATVRATFKRVQFTPDLMPADIVGTNIFDLPTGRFTLHPGPIFANIL
                     LGDEINRAPAKTQSALLEAMEERQVTIDGVSHDLPVPFYVIATQNPVEYEGTYPLPES
                     QLDRFLFKLLVEYPGQADETQLLRNYNRGFDARDLAGAGVQAVADPAALVAARQEMAA
                     VRADDAHLTYIAEIAEASRRAPDLLLGGSPRASVALLTAAKVYAALQGRDYLVPDDIK
                     TLAPAVYRHRIILKPEAEIEGLTPDRAIERILGRVPVPR"
BASE COUNT          697 a         1299 c         1435 g          818 t
ORIGIN      
        1 cggtttgaaa ccggccccta caccgatacc ttgctaactc gcgatgagcg gcccggcggg
       61 tctttcaaac cggccctaca ccgcatccca cggctaaccc gcgatgagtg gcgcgggcgg
      121 gtttcaaacc ggcccctaca ccgctggacg attgtggcct cctaccagcc cgcgcaggcg
      181 ggctttgttt gcgtagcccg ccccttccag gggccgggca gagaccgcac aaaaaaggat
      241 cggcccgccc ggaggcgagc cgatccacta aggccggcgc ggagaccgcg ccgatcccta
      301 gattacttgc ccagatacca catctgcgga tccgccaggt agccaccggc gtgcgcctgc
      361 aggttctgga agttgttcgt cgcgatgtag agcgacggca gaccgaacat cgggaaggtg
      421 gggatatcct tcgagacggc gatctgctgg cgcaggaacg actggatgcg atccgaagtg
      481 cgaccgatct gggtcagcgt gtcgcggcca tacttgtcgg catccgcatt gctccagaac
      541 atatcgttgc cgccgccgaa gccgttcccg gcactcggga tctgcgtgct gtcgaacagg
      601 ccggtggggt cgatctcgtc gctcgtgaac tgccacgcga actcgatcac accgaagttg
      661 ccgtagctgc cgctgtaaag cgggccatcc ggcgggttgg cgaagacctg gccggccgcc
      721 tgcacgtcag gcgtcgagtc gaaaccgacc gccttcagat actgctgctg ggccgtggcg
      781 atcagcttgc gatacgcctt gctcgtcgtc agccagtcca ccttcagcgt cttgccgttc
      841 ttgctgcgga tgccgtcggc ccccttgacc cagcccgcgg cgtccagcag ttcgttggcc
      901 ttggccacgt tgtagtcgta cttgacgatc tggctggtca ggtccgggta cttcttcatg
      961 acgtcagggt tctcggccga ggcccagtgg gtcggcggga tccaggcgtt catgatggtc
     1021 aactggccgc cgaagatggc gttgttgatg gcgttgcggt cgagcgagta ggccagggcc
     1081 tggcgcacgc gcacatcgtt gaacggctcc ttggtgacgt tgaagtcgat gtgctcatag
     1141 ctgtatccgc cctggttgag cagcttaccc ttgcccgcct tctcgaccgt ggtctggatc
     1201 gaggtgatca ggtcgggggt gatactctcg gaggtggtca ggtcgagcgt gcctgtggcg
     1261 atgcccaccg gcaactgctt gttgtcggag aacagcggcc aactgacctt ggcgaaggac
     1321 ggcttgtcgg gcgtgacact gtagttgggg ttgcgctcca tttcgagcgt ctggcccgtc
     1381 tggaacttgg tcacgtggta cggcccggtg ccccagaccg cctgcgagta gtcgctgctg
     1441 tcgatgtcgc caggggccat cttcgccagc gcgtgctgcg gcaggatgaa gcccaggact
     1501 tcgttatagg tcgggttaat caccgggatc ttcttgtcca cgaaggcctg caggaaggcg
     1561 taggtgtcct tatcgcgggc gatgagggcg gcggcttcct tccaggtcag gtagttgaag
     1621 atcagcgtgt agggatcggg gttgtcgacc gaggcgaagt actgtgcggg ctggcggccg
     1681 gccgccggga agtcggggtt caggaccagt ttgtaggtga agatgacgtc gttggagtcg
     1741 aagggctggc cgtcgaacca cttgacgcct ctcttcagct tgtacttgac caccaggcgc
     1801 ttgtcgtcgc cggtgcccac gtagtaggcg ccgccgttat cgagcgtcgg cacataggcg
     1861 gccaggtcgg gcacgacgtt ttccttgtcg tcgcgcttgg tcaggccgtt gtagacgaag
     1921 ttgcggacat tggtaccgat gagcgtgttg tcatcccagg ggatcaacgt ggccgggtcc
     1981 tggccgcgcc cgatgcgcag cacgtccacc gggttgttgc ccttggcgac ggccttctcc
     2041 atcaccggga cctggccctt ctgctcggcg ccgaggcggc ccagcagcac ctcaaacggg
     2101 gtgccggcgt tctccgggtg atactcgaag cgctggcgct cgaagtactg gaccaggaag
     2161 ctcttgccgg cgaccttggg atcggtctcg cgggtgactt cctggaacgg ctgactgatc
     2221 gggaagccga actggctcag gccgcccttc tggttccaga acttggcgat ggccttgccg
     2281 ccggtgctgc tgtcgccgag ggtgtgcttg gtctcgggga accactgcac gccgccgccg
     2341 ggattgtcga cggtcttgaa cggggtgtcc ttgcggcctt cagcggcgaa cacgccgagc
     2401 agaccgagaa gcacgtcata cggcttctgg ttttcggggt gcgcctcgaa gcgggcgcgc
     2461 tcgaaccact gcgtcttgta gatcttgccg tcggtgaggg agatctcatc gtgcacatcg
     2521 gtcagcggga gaccgttgat gtagaggcta tccccatagc tgcgtccgct ctcccagact
     2581 tcgaggaacc ggccggacac atcctgattg gtctcaggga agtgccgggt gccgccctgt
     2641 tgggccgggg cggcctggga ggccatcggc agggcagtta cggccatggc ggcaaccatt
     2701 gccccataga ctaacttccg cccaaatccg ctcgttgagc tcattaaggt tctcctcctg
     2761 ctaacgtgga ctgcgggaag cagatccgtg gatacgtgga cctaaggcag gtccgctagg
     2821 ccggcgcgct attgagccgg cacaagcagg ggtcgcttga ccataccaaa cgacacccgg
     2881 aatccgtgtg agaacttatc cgctccaggc gccaggaggg tcgctgtggg gtagagagac
     2941 cgcaaagacc caggcgcgtg gacaaccacc ccctttgcta gttgttgcgt gccttctccg
     3001 atgcatgtaa ccggccggac cggcctggcc cggcgccatc gcactatacc acatgagagg
     3061 cgagtttgca acactagagc ctgcccgccg gtcccagatg gtcgtgccgt cgaatgggaa
     3121 ccccggtagc cggggtagcc ccattcggga tcaacgcagg cgataccctt ggggacgccc
     3181 actaataggg gtacaacatc cggggccaaa tggttcaaat tcgggcccac gagtttttgc
     3241 ggcctagcgc ggcaccggca cccgccccag gatgcgctca atcgcccggt cgggggtcag
     3301 gccctcgatc tcggcttcgg gcttgaggat gatgcggtgg cggtagacgg cgggggccag
     3361 ggtcttgatg tcgtcgggca ccaggtaatc gcggccttgc agcgcggcgt agaccttggc
     3421 cgcggtcagc agcgccacgg aggcacgcgg gctgccgccc agcagcaggt cgggtgcccg
     3481 ccggctggcc tcggcgatct cggcgatgta ggtcaggtgc gcgtcgtcgg cgcggacggc
     3541 ggccatctcc tgccgggcgg cgacgagggc cgcagggtcg gccacggcct gcacccccgc
     3601 cccagccagg tcgcgcgcgt cgaagccgcg gttgtagttg cgcagcagtt gcgtttcgtc
     3661 ggcctgcccc ggatactcga ccagcagctt gaacaggaag cggtccaact gcgattccgg
     3721 cagtggatag gtgccctcgt actcgacggg gttttgcgtg gcgatgacgt agaaaggcac
     3781 gggcaggtca tgagacacgc catcgatagt cacctgccgc tcctccattg cttcgagcag
     3841 cgccgactgg gttttggccg gggcgcggtt gatctcatcg cccagcagga tattggcgaa
     3901 gatgggaccg gggtgcaggg tgaagcgccc ggtcggcaga tcgaagatgt tggtgccgac
     3961 gatatcggcg ggcatcaggt cgggcgtgaa ctggacgcgc ttgaatgtcg cgcgcacggt
     4021 cgcggccagg gtcttcgcca tcaacgtctt ggcgatgccc ggcacacctt cgagcagcac
     4081 gtggccgccg gccagcagcg ccacgatcag caactcgaag gtcgcatcct gtcccacgat
     4141 caccttgcgg cgttcccggt cgatggcctg aaagagttcg gcgacagtca cacgcggtcc
     4201 tgagtgccgg gtgctgagtg ccgagtcgtt ttcaaattgc atttgtcac
//
LOCUS       DASUYC010000020         2526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1715989, whole genome shotgun sequence.
ACCESSION   DASUYC010000020 DASUYC010000000
VERSION     DASUYC010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1715989"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..809)
                     /locus_tag="VF276_00275"
     CDS             complement(12..809)
                     /locus_tag="VF276_00275"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HEX6605326.1"
                     /translation="MGAPPPAPAAPVSAGPAARPAGYGKADLHIHSTYSDGIDSIPKI
                     LEFVEHQTDLDIIAIADHDDVRGAHEARELVARRGYRVQVIMGTEITTRQGHLLALFV
                     ERDFPMLKSLRASVDMIYEAGGLVIAPHPLCKLTASIQEKALLEVHHSAAPFHGMETF
                     NPSVAGRVSHREVAALNAQLHLPEFGGSDAHALSMIGMGYTYFPGSTVEDLKAAIAAR
                     TLIAGGRFMNFRDHAVIAAPNLWRSMIVSPAYKVQRAVRRGVRGQAT"
     gene            complement(1244..1510)
                     /locus_tag="VF276_00280"
     CDS             complement(1244..1510)
                     /locus_tag="VF276_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017740418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX6605327.1"
                     /translation="MAKHDLDWEVPEARQGQQRRERYLAGAIARAEYKVLDDDTVFGE
                     IPGFQGVWANAATEAACRMELRSVLDGWLTLRLDWHDPIPTQSS"
     gene            1807..2406
                     /gene="recR"
                     /locus_tag="VF276_00285"
     CDS             1807..2406
                     /gene="recR"
                     /locus_tag="VF276_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX6605328.1"
                     /translation="MQSTPEPIARLIGEFSRLPGIGPKSASRLAFYLLRHSREQAEAL
                     AVAITDVKDKIIFCTVCHNVTEEDPCAICSDPHRDRGKICVVEEPLDVMALERTGTYK
                     GLYHVLHGAIQPLEGITPDKLRIPELLRRVKLGDVDEVILATNPNMEGEATAMYLARQ
                     LVPLGVRVTRLARGLPIGGDLEYADEVTLARAIEGRREM"
BASE COUNT          474 a          802 c          808 g          442 t
ORIGIN      
        1 caggcaaatc ttcatgtcgc ctgcccgcgg acgccccggc gcaccgctcg ttggaccttg
       61 taggccgggg acacgatcat gctgcgccag agattaggcg cggcgataac ggcgtgatcg
      121 cggaagttca tgaagcggcc accggcgatg agcgtgcgcg ccgcgatcgc cgccttcaga
      181 tcctccactg tcgaaccggg aaaataggtg tagcccatgc cgatcatcga cagggcatgg
      241 gcatcgctgc cgccgaactc gggcaagtgg agttgggcgt tcagcgcggc gacctcgcgg
      301 tgcgagacgc gcccggccac gctggggttg aaggtctcca tgccgtggaa cggcgccgcg
      361 ctgtgatgca cctccagcaa cgccttttcc tggatgctgg ccgtcagttt gcagagcgga
      421 tgcggcgcga tcaccagccc gcccgcctcg taaatcatgt ccaccgaggc gcgcaacgat
      481 ttcagcatcg ggaagtcgcg ctcgacaaac agcgccagca ggtggccctg gcgggtggtg
      541 atctcggtgc ccatgatcac ctgcacgcga tatccccgcc gcgcaacgag ttcgcgcgcc
      601 tcgtgcgcgc cgcgcacgtc gtcatggtcg gcgatggcga taatatccag gtcggtctga
      661 tgctcaacga actccaggat cttggggatc gagtcgatcc cgtcgctgta ggtggagtga
      721 atatgcagat ccgctttgcc gtagccggcg ggccgggcgg cggggccggc ggagaccggc
      781 gcagcggggg cgggtggggg agcgcccatg gtcacgttgg ttgagtcctt tccatcgggc
      841 agcgcccggc taaaatcggc gagtcccggc ctgatccgcg gggatcgccc ctggcgctgt
      901 cgcaacctcc attataccag attcagcaac cgggaatccc gcccgcgcgg cacggtcagc
      961 ggggcgcagg ggcgcgggca ccccgttact ataccacaac tgtggccccg gcgggccggc
     1021 tagaggtagc cacgaaccgc gcgtggagcg gcgcggccag ggaaggcccg ctataagtct
     1081 agcgcagggt ccaatgacct attgggtgta gcggcgcgat tgatcgcgcc cgtactcgtt
     1141 agtctttagc gtgtgctgac cccaacaggt cgctagaccc tgcaccaggt caataccggc
     1201 ctatctcaag attgccagca acaagctttt ggagatgccg ccactatgag gattgggtgg
     1261 gaatcgggtc gtgccagtcc agtcgcaagg tcagccatcc atccaggacg gagcgcaatt
     1321 ccatgcggca ggcagcctcc gtagccgcat tggcccacac accctggaag ccgggaattt
     1381 caccgaacac ggtatcgtca tccaggactt tatactccgc ccgtgcgatg gccccggcga
     1441 gatagcgttc ccggcgctgc tggccctggc gggcctccgg cacttcccaa tccagatcat
     1501 gtttcgccat gccctgcctc gcttccattg ccaaagtaag ctgaacagcc gccgccgcgt
     1561 gtctccaaag agagaacgtc gcgtcaggtg cctggatttc cattatagca taggcaggtg
     1621 caaatacaac tagaaactgg acgtatcacg cgatctcatg cgggaggcgg aaggacgacg
     1681 ctttccggtt ttacgccaca gtatggtata attataggta cgaaattgat ccggggccgg
     1741 gtatagatag cccgcgcccg cgctgtgatt gtgtgttaag cttgagctag agagatgtga
     1801 ggatcgatgc agtccacccc tgagccgatt gcccgcctga ttggcgaatt cagccgcttg
     1861 ccggggatcg gccccaagag cgcgtcgcgg ctggcgtttt acctgctgcg ccacagccgg
     1921 gagcaggccg aggccctggc cgtggcgatc accgatgtga aagataagat catcttctgc
     1981 accgtctgcc acaatgtgac ggaggaagat ccgtgcgcca tttgctccga cccgcaccgc
     2041 gaccggggca agatctgcgt ggtcgaggag ccgctggacg tgatggccct ggagcgcacc
     2101 ggcacctata agggcctcta ccacgtgctg catggcgcca tccagccgct cgaaggcatc
     2161 acgcccgaca agctgcgcat ccccgaactg ctgcgccgcg tgaagctggg cgatgtcgat
     2221 gaggtgatcc tggcgaccaa tccgaatatg gagggcgagg ccacggccat gtacctggcc
     2281 cgccaactgg tgcccctggg cgtgcgcgtc acccgcctgg cgcgcggcct gcccatcggc
     2341 ggcgacctcg aatacgccga cgaagtcacc ctggcccgcg ccatcgaagg ccggcgcgaa
     2401 atgtagacga gcggtcagcc gtcagccgtc aggaactgtt tgagagcgtg cccatgaaca
     2461 aatggcacgc tctcacgctt tccggtacag gagtccccaa cgggttccac ctgatagctg
     2521 accacc
//
LOCUS       DASUYC010000021         3343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1946813, whole genome shotgun sequence.
ACCESSION   DASUYC010000021 DASUYC010000000
VERSION     DASUYC010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1946813"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1421)
                     /locus_tag="VF276_00290"
     CDS             complement(<1..1421)
                     /locus_tag="VF276_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605329.1"
                     /translation="MSRARVAWMAAGAALLLLVALAVVLAGDRLPGVELRQRDVALLH
                     AITDTATAAPEARRLGQTFTSEHDNLSAVALYLQNFSFRLPPEGRFHLLAGDSPTAPE
                     IVSLPLSAADFRDNPYLVFRFPPQPGSAGRTYRAWIETAAPLAATLSVHLTRVDAYSA
                     GAALIDDVPQPGDLEFRTFYTYTPASLVRDVWGSLVTGWRLGLAALAFLLLPGLVLAH
                     MLGRGWTPGVRLLAAPALTLFAWPVALLVGWTLHLDWGVAAAWLFVLAGVVGLVWTVY
                     RRAAAGGADPAPGGPATLRLTPIPTRPASLRRISVPTATERSRPGATTRLYWILFAAV
                     LGVTLISRLATIRDLVAGMGLDAYHHTLVTALFLDRGGVPHDYQPYAPLASFTYHFGF
                     HAFAAALAWLAPGSLPVERLMVLAGQLATTLPVVTLPLFAWRVLGNRWVGLVAGALAG
                     VVCIFPAFYVNWSRYTQGLGLALL"
     gene            complement(1418..2491)
                     /locus_tag="VF276_00295"
     CDS             complement(1418..2491)
                     /locus_tag="VF276_00295"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX6605330.1"
                     /translation="MNELQILFLEAGKQSAADVAGRLAGFLAQARQTIDIAIYDMHLT
                     GAARDLVVNALHERRRAGVQIRLCYDSGDNCQGADCLGDEPETTLTSTFLADAGLNGK
                     PIASQTHLMHQKYVILDAYTPQAQVWTGSSNFTDDSWTIQENNILMVRSPALANFYAQ
                     DFQELWSTADIQTTGAMDTGHVTLSYDGAPAPTTVSFSPGEGPWIDTEIAHRIGRARN
                     SVTLAFAVLTSGHILGALGDLAQQGVPVDGVYDRTQMEGVFAQWAQNPHSGWKTPAFE
                     SLVRYGHLVGKITTPWTPASPHDFMHNKVIVVDDTVITGSYNFSRNAECNAENILLID
                     SPALAAAYRTYIRHLADRYGQAG"
     gene            2673..>3343
                     /locus_tag="VF276_00300"
     CDS             2673..>3343
                     /locus_tag="VF276_00300"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6605331.1"
                     /translation="MTTSNSGGGDVALVPLTEDQMDEITDVMNRAFADYVVPMHLPVA
                     ALQAMIARDNIDLGQSYLARAADGTGLGVALTAVRCAGDEVRSRVAAMGVTPEGRGQG
                     LARRLLERQIADARARGSREILLECFSNNTRALPLYESAGFRVRRRLVSYEGDVAGLR
                     LPSSAAVTLRPVAPADLPALAARCAEAEPPWQMEPSTLATLNPATPARTIHLPDVPEP
                     AGYLVL"
BASE COUNT          507 a         1070 c         1272 g          494 t
ORIGIN      
        1 agcagggcca gccccaggcc ttgcgtgtaa cggctccagt tgacataaaa cgcgggaaag
       61 atgcagacca ccccggccag cgcccccgcc actaggccca cccagcgatt gcccagcaca
      121 cgccacgcga acagcggcag cgtgaccacc ggcagggtgg tggcgagttg cccggccagc
      181 accatcagcc gctcgaccgg caggctgccg ggggccagcc aggccagcgc ggcggcgaag
      241 gcatggaagc cgaagtggta ggtgaacgag gccagcggcg cgtagggctg gtagtcgtgg
      301 ggcacgccgc cccggtcgag aaacagcgcc gtgaccaggg tgtggtgata agcgtccagg
      361 cccatcccgg ctaccaggtc gcggatggtc gccagccgcg agatgagcgt cacgccgagg
      421 acggcggcga aaaggatcca gtagagccgg gtggtggcac ccggtctgct gcgctcggta
      481 gccgtcggga cacttatccg ccgcaggctg gccgggcgcg tcgggatggg cgtcagccgc
      541 agggtcgcag ggccgcccgg cgcgggatcg gcgccgccgg cagccgcgcg ccggtagacg
      601 gtccacacca gtcccaccac gccggccagg acgaacagcc aggcggcggc cactccccaa
      661 tccagatgta gcgtccagcc caccagcagc gccactggcc aggcgaacag ggtcagcgcg
      721 ggggcggcca gcaggcggac gccgggcgtc cagccccggc ccagcatgtg cgcgagaacc
      781 agtcccggca gcagcaggaa ggccagcgcg gccagcccga gccgccaccc cgtgaccagg
      841 ctgccccaca catcgcggac cagggatgcc ggggtgtagg tgtagaaggt gcggaactcc
      901 aggtcgccgg gctggggcac gtcgtcgatc agggccgcgc ccgccgaata ggcgtccacg
      961 cgtgtcaggt ggaccgagag ggtcgctgcc agcggcgcgg cggtctcgat ccaggcgcgg
     1021 taggtgcgcc cggcggagcc gggttgcggc gggaagcgga agaccaggta ggggttgtcg
     1081 cggaagtcgg cggcgctgag cggcaggctc acgatctccg gcgcggtggg gctatcaccc
     1141 gccagcaggt ggaagcgccc ctccggcggc aggcgaaagc tgaagttttg cagatacagg
     1201 gcgacggcgc tgagattgtc gtgttcggac gtgaatgtct ggcccaggcg gcgggcctcg
     1261 ggcgcggcgg tggccgtgtc ggtgatcgcg tgcagcaggg ccacgtcgcg ctggcggagc
     1321 tcgacgcccg gcagccggtc cccggcgagc acgactgcca gggcgaccag caggaggagc
     1381 gccgccccgg cggccatcca tgcgacgcgc gctcggctca accagcctgc ccgtagcggt
     1441 ccgcgaggtg gcggatatag gtccggtagg cggcggccag ggccgggctg tcgatcagca
     1501 ggatgttctc ggcgttgcac tcggcgttgc gcgagaagtt atagctgccg gtgatcaccg
     1561 tgtcgtctac aacgatcact ttgttgtgca tgaaatcgtg cgggctggcc ggcgtccagg
     1621 gcgtggtgat tttgcccacc aggtggccgt agcgcaccag cgactcgaaa gccggggtct
     1681 tccacccaga gtgcgggttc tgtgcccact gcgcgaacac gccttccatc tgcgtgcggt
     1741 cgtagacgcc gtccaccggg acgccttgct gggccaggtc gccgagcgcg cccaggatat
     1801 ggccggaggt gagcacggcg aaggcgagcg tgacgctgtt gcgcgcccgc ccgatgcggt
     1861 gggcgatctc tgtgtcgatc caggggcctt cgcccggcga gaagctgacc gtggtgggcg
     1921 cgggggcgcc gtcatagctc agcgtgacgt ggccggtgtc catggcgccg gtggtctgga
     1981 tgtcggcggt ggaccagagt tcctggaagt cctgcgcgta gaagttggcg agggcgggcg
     2041 agcgcaccat caggatgttg ttttcctgga tggtccagct atcgtcggtg aagttgctgg
     2101 agccggtcca gacctgggct tgcggcgtgt aggcgtcgag gatgacgtac ttctggtgca
     2161 ttaggtgggt ctggctggcg atgggcttgc cgttcagccc ggcgtcggcc aggaaggtcg
     2221 aggtcagggt cgtctcgggt tcgtcgccca ggcagtccgc gccctggcaa ttgtcgccgg
     2281 agtcgtagca gagccggatc tgcacgcccg cccgccgccg ctcgtgcagc gcgttgacca
     2341 ccaggtcgcg ggccgcaccg gtgaggtgca tgtcgtagat ggcgatgtcg atggtctgcc
     2401 gcgcctgggc caggaacccg gccaggcgcc ccgccacgtc ggcggcggac tgcttgccgg
     2461 cttccagaaa gaggatctgc aactcgttca cgatgtctct ccttccgctt gggggccaac
     2521 ctctcccccg gcgggggtat accccaccgc ggagagggaa gttcggcgcg acgagagcga
     2581 acgttggcgg cgtttcgccc ttccattata ctgatcggac gcgcccttgt gcagggcggt
     2641 gatccggcta tgcgtttgaa ggagcaattt tcgtgacaac atcgaattcg gggggcggcg
     2701 atgtggcgct cgttccgtta actgaagacc agatggacga aatcaccgat gtgatgaacc
     2761 gcgcctttgc cgactacgtg gtgccgatgc acctgccggt ggcggcgttg caggcgatga
     2821 tcgcgcggga taatatcgac ctggggcaat cgtatctcgc gcgggcggcg gacggcaccg
     2881 gcctgggcgt ggcgctcacg gcggtgcggt gcgcgggaga cgaggtgcgg tcgcgggtgg
     2941 cggcgatggg ggtgacgccg gaggggcgcg ggcaggggct ggcccgccgc ctgctggagc
     3001 ggcagatcgc cgatgcgcga gcgcgcggct cgcgggagat cctgctggag tgcttcagca
     3061 acaacacacg cgccctgccg ctctacgaga gcgcggggtt tcgcgtgcgc cgccggttgg
     3121 tcagctacga gggcgacgtg gcgggcttgc gcctgccctc gtccgcggcg gtgacgctgc
     3181 ggcccgtcgc ccccgccgat ctgcctgcct tggcggcgcg ctgcgcggag gccgagccgc
     3241 cctggcaaat ggagccgtcc acgctggcga cgctcaaccc ggccacgccc gcgcggacga
     3301 ttcacctgcc ggatgtgccg gagccggcgg gctacctggt cct
//
LOCUS       DASUYC010000022         4301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_426280,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000022 DASUYC010000000
VERSION     DASUYC010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426280"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1409
                     /locus_tag="VF276_00305"
     CDS             <1..1409
                     /locus_tag="VF276_00305"
                     /inference="COORDINATES: protein motif:HMM:NF028228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6605332.1"
                     /translation="IEYIPGQKREAWGTEGYAPPEIGGICEQPPTMDLFTIVSTLAAL
                     LGLDVAEQRILAPPHESWPVSQEIYDFVVRGRDPDPSVRFQTVDELFDQLEGINRFIK
                     GQELARVAGPAQLPGRGTSGLAQAIAPVQSTLFTGKLLTGTTGKISALPALNTSDPAA
                     PALQPARDLMAAGRYSEALTQIDAALKTNPASIDGHLLKAASLSNLGRADEARDELDR
                     AQRLSTPATRWRTILVQAQAAVAVRDFARAEQLYQELMKLVPGEILPKQALADIYRDT
                     GNYGLAADLYSRVVSADPANADAILGWADSLVALNRTDQAIGVLDAVNENALRFVDAQ
                     LRLIELYLMRIDAYLTQQDGALARGKPAPPGVPALLHDLGLAARALAGLNDRTESRHY
                     YRLLADWWYAAYRLARVGRLPANIEWPDGQVYHADRPAEIGQACREAYRRYLRRAPDA
                     PDREDILARIYFDVTEWQ"
     gene            1476..2768
                     /locus_tag="VF276_00310"
     CDS             1476..2768
                     /locus_tag="VF276_00310"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX6605333.1"
                     /translation="MVTFRVIPHPAEEQVLQGQGLARVLYTVGAEGAAGESVPVFLGV
                     VMDQSGSMSGSKLEAAKDALLHLLHQVPPTDDVVVHITLFSTSAHELAPPITGRALAG
                     QMPELQRRIRRIDAGGSTSLGSGLRVAMGAANYYPNYVRRVLMMTDGKQEGAEPISDA
                     YQTAENLGRAGIRVDAWGVGNAWEADELRTIAHSTGGEAEAVPNARDLGDAVAELFAD
                     VQSTKASDVRLVLTTPKGTLIRSVRQVYPSVQERAASRLDEQRWVIPIGSLTQEEPKF
                     VVELETTPRNVGISFRMLVPTVTYSQGGRDQTDELGRDSWFFVRWVATPAEVRMDPEL
                     SRFTGEEEITSLSQEGFALLEMGAVEEATAKLNAALRKAEQINSPQVATLGAVVNPQT
                     GRLVSTDANAAVNKTGKLHTGKTGKLLAGTGKLPGKGS"
     gene            2867..4243
                     /locus_tag="VF276_00315"
     CDS             2867..4243
                     /locus_tag="VF276_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605334.1"
                     /translation="MGNPRIARLRDRMLTLISAVVAFGLLAGIIAGLASSTTYDAYHT
                     LVDEGAVSVDAALRARAAVLDHMSAAATYLATTGPAHDAAYAQAINSWDTYREQSRLS
                     WRNISDPVQGEGSVYQAADRAASDYIQQIGAMFAYDRAGQKDQAGAAFLAARETLNSR
                     LVPALGGLEAVKVEDMEATYAGAAGRITNWRYAVIAMTVLLSLILLGGLWAVRRMHYR
                     WSWPLGVALLLTIAVGAWTQEQLARAATEARVMVRDAHDTVAGVQDLAALLSQERALQ
                     SIAIFDPTHAATHLASFEQYNFLVEQKLCGPTDCTATTFLSGPDTIDPQVEQAALDAQ
                     AKFGLPRTPLIANVHFPGQAAEFEALRQAYRTWLDQHRNNLAQALAAGDVDAARTLST
                     GSAATNFAAVVKAADAVRDTARTRFDDIWRTVYFTSTLDQGLALAFPIAGLLGAWGLW
                     RRRTELFI"
BASE COUNT          680 a         1603 c         1445 g          573 t
ORIGIN      
        1 ccatcgagta tatccccggc cagaagcgcg aagcctgggg caccgagggc tatgccccgc
       61 ccgagatcgg cggcatctgc gagcagccac ccacgatgga cctgttcacc atcgtcagca
      121 cgctggccgc gctgctcggg ctggacgtgg ccgagcagcg catcctggcg ccgccgcacg
      181 aatcctggcc cgtctcgcag gagatctacg acttcgtggt gcgcgggcgc gaccccgatc
      241 ccagcgtgcg cttccagacg gtcgatgagc tgttcgacca attggagggc atcaaccgct
      301 tcatcaaggg ccaggaactg gcccgcgtgg ccggccccgc gcagttgccg ggtcggggca
      361 ccagcgggct ggcccaggcc atcgccccgg tgcagagcac cctgttcacc ggcaagctgc
      421 tgacgggcac gaccggcaag atcagcgcgc tgcccgcgct caacacgtcg gacccggcgg
      481 cgcccgcctt gcagcccgcc cgcgacctga tggcggcggg ccgctattcc gaggcgctga
      541 cccagatcga cgccgcgctc aagaccaacc cggccagcat cgacggccat ttgctgaagg
      601 cggccagcct gagcaacctg ggccgcgccg acgaggcccg cgacgaactc gaccgcgcgc
      661 aacgcctgag caccccggcc acgcgctggc gcaccatcct ggtgcaggcc caggccgccg
      721 tcgcggtgcg cgactttgcg cgggccgagc agctctacca ggagttgatg aagctggtgc
      781 cgggcgagat cttgcccaag caggccctgg ccgatattta ccgcgacacc ggcaactatg
      841 gcctggccgc cgacctctac agccgcgtcg tctccgccga cccggccaac gccgacgcca
      901 tcctgggctg ggccgacagc ctggtggcgc tgaaccgcac cgaccaggcc atcggcgtgc
      961 tggacgccgt caacgagaac gcgctgcgct tcgtcgacgc ccaactgcgc ctgatcgagc
     1021 tgtacctgat gcgcatcgac gcctacctga cgcagcaaga cggcgccctg gcgcgcggca
     1081 agcccgcgcc gcccggcgtg cccgcgctgt tgcacgacct gggtctggcc gcccgcgccc
     1141 tggccgggct caacgaccgc accgagtcgc gccactacta tcgcctgctg gccgattggt
     1201 ggtacgcggc ctaccggctg gcccgcgtgg gccgcctgcc ggccaacatc gagtggcccg
     1261 acggccaggt gtaccatgcc gaccggcccg ccgagatcgg gcaggcgtgc cgcgaggcct
     1321 accggcgcta cctgcgccgc gcgcccgacg cgcccgaccg cgaggacatc ctggcgcgca
     1381 tctatttcga cgttaccgag tggcagtagc cgagttggca tagcgagggc gcggcgcggc
     1441 ggttgccggt cgggctgtaa ggaggcttgt cgttcatggt tacctttcgg gtcatccccc
     1501 atcccgccga ggaacaggtg ttgcagggcc agggcctggc ccgcgtgctc tacacggtcg
     1561 gcgccgaagg cgcggcgggc gagagcgtgc cggtgttcct gggcgtggtc atggaccagt
     1621 ccggcagcat gagcggcagc aagctggagg cggcgaagga cgccctgctc catctgctgc
     1681 accaggtgcc gccgaccgac gacgtggtgg tgcatatcac gctgttcagc acctcggccc
     1741 acgaactggc cccgccgatc accgggcgcg cgctggccgg gcagatgccc gaactccagc
     1801 gccgcatccg ccgcatcgac gcgggcggct cgacctcgct gggcagcggc ctgcgcgtgg
     1861 cgatgggcgc ggccaactat taccccaact acgtccgccg cgtgctgatg atgaccgacg
     1921 gcaagcagga gggcgccgag ccgatcagcg atgcctacca gacggccgag aacctgggcc
     1981 gcgcgggcat ccgcgtggac gcctggggcg tgggcaacgc ctgggaggcc gacgaactgc
     2041 gcaccatcgc ccacagcacc ggcggcgagg ccgaagccgt gcccaacgcc cgcgacctgg
     2101 gcgacgccgt ggccgagctc ttcgccgacg tgcagagcac caaggccagc gacgtgcgcc
     2161 tggtgctgac tacgcccaag ggcacgctga tccgcagcgt gcgccaggtc tatccctcgg
     2221 tgcaagagcg cgccgccagc cgcctggacg agcagcgctg ggtgatcccc atcggctcgc
     2281 tcacgcagga agagccgaaa ttcgtcgtgg aactggagac caccccgcgc aacgtaggca
     2341 tctccttccg catgctggtg cccaccgtga cctattcgca gggcgggcgc gaccagaccg
     2401 acgaactggg ccgcgactcc tggttcttcg tgcgctgggt ggcgacgccc gccgaggtgc
     2461 gcatggaccc cgagctctcg cgcttcaccg gcgaggagga gatcaccagc ctgtcgcaag
     2521 agggcttcgc gctgctcgaa atgggcgccg tggaggaagc cacggccaag ctcaacgccg
     2581 cgctgcgcaa agccgagcag atcaacagtc cgcaggtggc aacgctgggc gctgtggtca
     2641 atccgcagac cgggcggctc gtgtcgaccg acgccaacgc cgccgtgaac aagaccggca
     2701 aactgcacac cggcaagacc ggcaaactgc tggccggcac cgggaagctg ccgggtaagg
     2761 gcagctaggg aggaccgtta ccatcgctac gatagccccg ccccaaccgg cccccgcgcc
     2821 cgccgcgccg ccgcaggcag cgcccgtccc ggcgccggcg ccgattgtgg gcaatccccg
     2881 catcgcccgg ctgcgcgacc gcatgctcac gctgatctcg gcggtggtgg cctttggcct
     2941 gctcgccggg atcatcgccg gactggcgag ttccaccacc tacgatgcct accataccct
     3001 cgtcgacgag ggcgcggtca gcgtggacgc cgcgctgcgt gcccgcgccg ccgtgctcga
     3061 ccacatgagc gccgccgcca cctacctggc gaccaccggc ccggcccacg acgcggccta
     3121 tgcccaggcc atcaatagct gggacaccta ccgcgagcag tcgcgcctgt cctggcgcaa
     3181 tatcagcgac ccggtgcagg gcgagggcag cgtgtaccag gcggcagacc gcgccgccag
     3241 cgactacatc cagcagatcg gcgctatgtt cgcctatgac cgggccgggc aaaaggacca
     3301 ggcgggcgcc gccttcctgg ccgcccgtga gacgctgaac agccgcctcg tgccggcgct
     3361 cggcggcctc gaagccgtca aggtcgagga tatggaggcg acctacgcgg gcgccgccgg
     3421 gcggatcacc aactggcgct acgccgtgat cgccatgacc gtgctgctca gcctgatcct
     3481 gctgggtggg ctgtgggccg tgcgccgcat gcactatcgc tggtcctggc cgctcggcgt
     3541 cgccctgctg ctgaccatcg cggtcggcgc gtggacgcag gagcaactgg cccgcgccgc
     3601 caccgaggcg cgcgtcatgg tccgcgacgc ccacgacacg gtcgccgggg tgcaggacct
     3661 cgccgccctg ctcagccagg agcgcgccct gcaaagcatc gccatcttcg acccgaccca
     3721 cgcggccacg cacctggcga gcttcgagca gtacaacttc ctggtcgagc aaaagctgtg
     3781 cggccccacc gactgcaccg cgaccacctt cttgagcggc ccggacacca tcgacccgca
     3841 ggtcgagcag gcggccctgg acgcgcaggc caagttcggc ctgccgcgca cgccgttgat
     3901 cgccaatgtc cacttccccg gccaggccgc cgagtttgag gcgctgcgcc aggcgtatcg
     3961 tacctggctc gaccagcatc gcaacaacct ggcgcaggcc ctggcggcgg gcgatgtgga
     4021 cgcggcgcgt acgctaagca ccggcagcgc ggcgaccaac ttcgccgccg tggtcaaggc
     4081 cgccgacgcc gtccgcgaca ccgcccgcac ccgcttcgac gacatctggc ggacggtcta
     4141 cttcaccagc acgctggacc agggcctggc gctggccttc cctatcgcgg gcctgctggg
     4201 cgcctggggc ctctggcgcc gccgcacgga actgtttatc tagcgcacaa caggtagcgc
     4261 gatgcagcga cgcccggcag gtttttcctc gccgggcgcc g
//
LOCUS       DASUYC010000023         4292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1021932, whole genome shotgun sequence.
ACCESSION   DASUYC010000023 DASUYC010000000
VERSION     DASUYC010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1021932"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..601
                     /locus_tag="VF276_00320"
     CDS             185..601
                     /locus_tag="VF276_00320"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX6605335.1"
                     /translation="MSAEANKAIVRRFIEGAWNEKNPAIADELCAPTYIYHGSDHEPG
                     PAIARQLIGMFAEAFPDAQATIDQILAEDDLVAMRWTTVGTHEGDFGGLPATGKRVQM
                     NGMEIFRLEDGLIVERWSNSDTLGLLQQLGVISPAE"
     gene            743..1552
                     /locus_tag="VF276_00325"
     CDS             743..1552
                     /locus_tag="VF276_00325"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HEX6605336.1"
                     /translation="MIEQPTNEAQAPMILRLEADPRDANRVVVTYGPPAADLSAARTL
                     LLHIAVVADEGLHTGQPCPPDLVARLQAGDEFQREYNRALNLLAVRPRSTAEVRDRLR
                     RKNVAPDMIDAVIARLQRAQYLDDAEFARYWIGERARSSPRGARLLRGELRNKGVSPA
                     LIDEALANFEAEAAAEHAARQAAAPADDSDGTEPDLVDGADSRELREALGLAQRKLRA
                     YSNLDPVTFRRRMSGFLLRRGYGYDVVSRVLKHIEATTSDQWSVVSGQDEE"
     gene            1732..2373
                     /locus_tag="VF276_00330"
     CDS             1732..2373
                     /locus_tag="VF276_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6605337.1"
                     /translation="MPDITWQGYASFRLRGKDATIVTDPFDRGLGLEVPRPRADIVTI
                     SRDEPGHNAEATVKGEPRVLRAPGEYEVKGVFINGVATYADAKKGALRGKNTVFVYEL
                     DDVVICHLGGLGHTLSAAQVEALSDVSVLIVPVGAPDALDAAKAVEVIGQIEPAIVIP
                     MMYKTGPETLNLEPLDRFAKEMGLKEWVAQDKLVIKSSDLPETTQVVVLEVKS"
     gene            2471..4057
                     /locus_tag="VF276_00335"
                     /pseudo
     CDS             2471..4057
                     /locus_tag="VF276_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189291.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
BASE COUNT          826 a         1421 c         1430 g          615 t
ORIGIN      
        1 ggcccggaca gcaagagcaa gaagggcgct gcccccgtcg tcgaggaaga ggacgacgag
       61 gagatctaag actggcctcc ggtgtatact acaccgcagt acctgaatag cagccggcac
      121 tggtaggggc gggtaccaac ccgcccctat ccctgttcag ccagggtccg aaggaggatc
      181 gcccatgtcc gcggaagcca acaaggccat cgtgcgccgc tttatcgagg gcgcgtggaa
      241 cgaaaagaac cccgccatcg ccgacgagtt gtgcgccccc acctacatct atcacggctc
      301 ggaccacgaa cccggccccg ccatcgcccg ccaactgatc ggcatgttcg cggaggcctt
      361 ccccgatgcc caggcgacca tcgaccagat cctggccgaa gacgacctgg tcgccatgcg
      421 ctggaccacg gtgggcacgc acgagggcga tttcggcggc ctgcccgcca cgggcaagcg
      481 cgtgcagatg aacggcatgg agatcttccg cctggaggac gggctgatcg tcgagcgctg
      541 gagcaacagc gacacgctgg gcctgctgca acagctcggc gtgatttcgc cggctgagta
      601 actccctgcg gtgaaggtgt gggggacagg ccggtttgaa accggcccct acagcgccaa
      661 acgggcgatc cggccggttt caaaccggcc cctacaccgg gcaattatgc cggctgaaca
      721 ctggaaagcg acagacggac ccatgatcga gcagccaacc aacgaagccc aggcacccat
      781 gatcctgcgg ctggaggccg acccgcgcga cgccaaccgg gtggtcgtga cctacgggcc
      841 gcccgccgcg gacctgagtg ccgcgcgcac gctgctgttg catatcgccg tagtggccga
      901 cgaagggctg cataccgggc aaccgtgccc gcccgacctg gtggcgcggt tgcaggcggg
      961 cgacgagttt cagcgcgagt ataatcgggc gctgaatttg ctggctgtgc gcccgcgcag
     1021 caccgccgag gtgcgcgacc ggctgcgccg caagaacgtg gcgcccgaca tgatcgacgc
     1081 cgtcatcgcc cgcctgcaac gcgcgcagta tctggacgat gccgagttcg cgcgctactg
     1141 gatcggcgag cgcgcccgca gcagtccgcg cggggcacgg ctcttgcgcg gagagttgcg
     1201 caacaagggt gtcagcccgg cgctgattga tgaagcgctg gcgaatttcg aggccgaggc
     1261 cgccgccgaa cacgccgccc ggcaagctgc ggcgccggct gacgatagcg atggcaccga
     1321 gccggatctt gtggatggcg cagatagccg cgaactgcgc gaggcgttgg ggctggccca
     1381 gcgcaagctg cgcgcctaca gcaacctcga tcccgtcacc ttccgccgcc gcatgagcgg
     1441 cttcctgctg cggcgcggct acggctacga tgtcgtgtcc cgcgtcttga agcacatcga
     1501 ggcgacgacg agcgatcagt ggtcagtggt cagtggtcag gatgaggaat gacgccaatg
     1561 gggcacggaa cctgtcattc tgagcgcagc gaagaatctc gtctcggacg accggcgacg
     1621 agactcttcg ccgtgcgaag agcgacaagt catgtgtgat actgaccact gatcactgac
     1681 cactggccac taatctggta taataagcag ttgagaggag attaaggcag catgccggat
     1741 attacatggc aaggctacgc gtcgttccgc ctgcgcggga aggatgccac catcgtcacc
     1801 gatcccttcg atcggggcct ggggctggaa gtgccgcgcc cgcgggccga catcgtgacc
     1861 atcagccggg acgagccggg ccacaacgcg gaggccacgg tcaagggcga gccgcgggtg
     1921 ttgcgcgcgc cgggcgagta cgaggtcaag ggcgtgttca tcaacggcgt cgcgacctat
     1981 gccgacgcca agaagggcgc cctgcgcggc aagaacaccg tcttcgtgta tgaactggac
     2041 gacgtggtca tttgccatct cggcgggctg ggccacaccc tgagcgccgc ccaggtcgag
     2101 gcgttgagcg atgtatcggt gttgatcgtg cccgtgggcg cgcccgatgc gttggacgcg
     2161 gcgaaagccg tcgaagtgat cgggcagatc gagccggcca tcgtgatccc gatgatgtac
     2221 aaaaccgggc cggaaaccct caatcttgaa ccgctggacc gattcgcgaa agagatgggc
     2281 ttgaaagagt gggtagccca ggataagctg gtcataaaaa gcagcgacct gccggagaca
     2341 acccaggtcg tagtgcttga agtcaaatca taacggggcg ggggccaggg gcgacaaccc
     2401 tctggcccct ctcctttttg gagacccgtc acgcacccgg cgcacggcgc ccggagagga
     2461 ggaacactta atggctaaat atattttcgt caccggcggg gtcgtgtcct ccgtcggcaa
     2521 gggcatcacc gtcgccagca tcgggcggat tctcaagagc cgcggcctgg gcgtctcggt
     2581 catgaagctc gacccctaca tcaacgtcga tcccggcacc atgtcgccct accagcacgg
     2641 cgaggtgttc gtgaccgacg acggcgcgga aaccgacctg gacgtgggcc actacgagcg
     2701 gttcatcgac gagaacatga cccgcgcggc caacatcacc acgggccaga tctactcgaa
     2761 cgtgatcggg cgcgagcgcc ggggcgactt ccttggcggc acgatccagg tggtgccgca
     2821 catcaccaac gagatcaagt cgcgcatcgc cgcggtggcc cgccagtcgg gggccgacgt
     2881 ggtgctggtc gaggtgggcg gcacggtcgg cgacatcgag ggcctgccct tcttcgaggc
     2941 gatccgccag atgcggcggg acgcgggccg cgaaaacgtg atgtatatcc acctgaccta
     3001 tctcccctac atcggcgcca cgcacgaact gaaaaccaag cccacccagc acagcgtcaa
     3061 ggaactgcgc tccatcggca tcaaccccaa cgtcatcctc tgccgcgcgg actacccggt
     3121 ggagccggac acactggcga agatcgccat gttctgcgac gtggaccagg acgccgtgat
     3181 cgagatgccg accgtgagca gcatctacat ggtgccgctg atcctggagg acgcgggcct
     3241 gggcaactac atcgtgaagc ggctggaact cgactgccac gcgcccgacc tggccgagtg
     3301 gcgccagttg gtcgagcgga ttcgcgcccc gaaggaaacg ctgtccatcg ccatctgcgg
     3361 caaatatacc gaactccacg acgcctacct cagcgtggcc gaggccctga agcacgccgg
     3421 gctgcaccac cgcgtcgacg tgaatatccg ctgggtgaac accgaggtgc tggagcagga
     3481 aggcagcgag cacttcctgc atgacgtgga cggcatcgtc gtgcccggcg gcttcggcga
     3541 ccggggtgtg cagggcaaaa tcatggccgc cgaatatgcc cgcgagcacg agatccccta
     3601 cctgggcctg tgctacggca tgcagatggc ggtgatcgaa ctcgcccggc acgtagccgg
     3661 cctgcccaac gccaacagca ccgagatcga cccgcgcacg ccgcacccgg tgatcgacat
     3721 catggccgac cagcgcgaca tcgccgacat cggcggcacg atgcgcctgg gcatctacgc
     3781 ctgcaagctg gtgcccggca ccaaagccgc cgccgcctac ggcacggacc aggtcaacga
     3841 gcgccatcgc catcgcttcg agttcaacaa cgcctaccgc gagcggctgg aggaggcagg
     3901 gctggtcatc agcggcgtct cgcccgacgg gcggctggtg gaagtgatcg agttgcgcga
     3961 ccatccctgg ttcgtcgcga gccagtttca cccggaactg aagtcgcgcc cgaccaaccc
     4021 gcacccgcta ttccgcgagt tcatcgccgc ggccatgcgc cggcaccagg cgcacccgga
     4081 cggcgtggtc gcagccgccg agccggcggg ggtgcccggc gaccgccaca acgggcgcgc
     4141 cgcccggccc gagccggtgg ccgcgggcgc cgccgagacc agggcagcgg acgcgcccgg
     4201 cgcatgaggc ccgcacccac cccgctgccc cggcgcgagc gcggcctgac ggtggacggg
     4261 ggcgaggaag ggctgctggc gggcaagctg ag
//
LOCUS       DASUYC010000024         5216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1252067, whole genome shotgun sequence.
ACCESSION   DASUYC010000024 DASUYC010000000
VERSION     DASUYC010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5216
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252067"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..1336)
                     /locus_tag="VF276_00340"
     CDS             complement(152..1336)
                     /locus_tag="VF276_00340"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX6605338.1"
                     /translation="MNRSSSNGPAEPGFNVEIRRGQRAVDQQAALHPAAGAKAGRRAP
                     EAEAAWGGVRVKRRVKLQQKILGGVLILAALVVAGFFALSWWSTQPPPGVLARVNGEN
                     ITVQEVDREILLNRALTALLTGDEQTLSRSATVESLIDRRMRAQDAARAGITVSDADI
                     DAFVQTLITRNGKTMDDLDTALKGYGLTRADLYADQRDVVAINSYIGLKVVAGATSDD
                     QRSQLMNDWITQLQQTSKVERFGTPDEPTAPRVGATAPDFKLRDINDKIYALSDFHGR
                     PVIVNFWATWCEPCRSELPTIQAAYARLQKEGPQPGPGLEVLGVAVDSDPSIVSSFQR
                     EFGLQFPLLPDMAGQGGVKDLYRVGPIPTSFFIDRQGIVRAVKIGALQEQDLTGDLKL
                     IQ"
     gene            complement(1341..2099)
                     /locus_tag="VF276_00345"
     CDS             complement(1341..2099)
                     /locus_tag="VF276_00345"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX6605339.1"
                     /translation="MPESNANIFVAVLAGFLSFASPCVLPLVPVYLGYMSGTSIQRGR
                     MQAARWRVFLHALAFVAGFSFIFVLLGASAGVVRDWLAEYRQPIQIVLGFLLLIFGLH
                     TLGVFQIPFLNYERRLELHPSKNLGYVRSVLIGTGFAIGWTPCVGPFLGLMYTLAMQE
                     RTTEAVPLFIAYSLGLGIPFILAALLAGQLTIWLRKVMMRSFDLRLAGRTLVAGLNPI
                     SLVSGVLLLFMGGLLMMDRVTWLNQFLPTWTLGI"
     gene            complement(2189..3280)
                     /locus_tag="VF276_00350"
     CDS             complement(2189..3280)
                     /locus_tag="VF276_00350"
                     /inference="COORDINATES: protein motif:HMM:NF021384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HEX6605340.1"
                     /translation="MPTITLYSKPNCPLCDHARHYLRQVIAERPDAPAWTVDEVSILD
                     DQDLYAAYRYVIPVVVVDGGTPIPAPASLDVAHLRAALDQGPARATPAPAMPADAPGA
                     TAPATGPGLDPAAGAASVPGDGSTGATVPAQAYPYPYYYGPPRPQTGVFGAFERFGNG
                     MTTHWLAVVNTAIGIFSTLPWLAPVFAALGWWFLADPIYTIYMFFCHQLPERAGNLFG
                     YQVAYCYRNSAIYTTIFLGGLLFAAARRGAFGGRLAWMLRPIRWQVFVVLLVPIAVDG
                     LTHMFGLREDNAWFDTLTGGRFGDFSVGDSAGTLNWWLRVISGAIFGFAVVRLVYPWV
                     LRAVEESRSVYWVPAAPVPPYEAPQTVST"
     gene            3789..5123
                     /locus_tag="VF276_00355"
     CDS             3789..5123
                     /locus_tag="VF276_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HEX6605341.1"
                     /translation="MVSTVKPTAETQQLPGPKAEALVARDDRVISPSYTRSYPFVMDH
                     GKGSEVWDVDGRRFIDFTAGVAVLVAGHAHPAIVEAITKQAQKYIHMAGTDFYLPEAV
                     ELAETLARITPGDYEKQVFFTNSGTESNEAAMKLCHWYTGRPAFISFLGAFHGRTYGS
                     MSLGSSKALHRARYQPLLSGIYHAPYPNPYRPPFDVPTERLGRACVDYIEHTMMKHLV
                     RPDEVAGIFVEPIQGEGGYVVPPGDFLPALRALCDKYDIPLVADEVQSGMGRTGKMFA
                     SEHWGVVPDVISIAKGIGGGLPLGAIVAPKRMMTWPSGAHANTFGGNPVACAAALATI
                     NLLETELLANTNEVGAQMMARLQTWPDRFPIVGEVRGRGLMLGIDLVEDRTTRTPAHD
                     LRDQIVDEAFHRGLLLLGAGAGAIRLCPPLILTQDLADEGLTILEDLLAAHS"
BASE COUNT          948 a         1734 c         1743 g          791 t
ORIGIN      
        1 gacggagatt cttcgctgcg ctcagaatga caaggcggcg ggagtggcta tgcagaatga
       61 cagggcgcct gtgacggatc acagcgcata aaagcgcccc tgacccgttt gtaccgggcc
      121 aggggcggca gggttgcgga aaaaacagcg cctactggat cagcttcaga tcgccggtca
      181 ggtcttgctc ttgcagcgcg ccgatcttca ccgcgcggac gatgccctgg cggtcgatga
      241 agaagctggt cgggatgggg ccgacgcgat agaggtcttt caccccgccc tggcccgcca
      301 tgtcgggcag cagcgggaac tgcaagccga actcacgctg gaaggaactg acgatcgacg
      361 ggtcgctgtc cacggccacg ccaagcactt cgaggcccgg tccaggctgc ggcccctctt
      421 tctgcaaccg tgcgtacgcg gcctggatgg tcggtagctc gctgcggcag ggctcgcacc
      481 aggtggccca gaagttcacg atgaccgggc ggccatggaa atcggacagc gcgtagatct
      541 tgtcgttaat gtcgcgcagc ttgaagtcgg gggccgtggc acccacgcgc ggcgccgtcg
      601 gctcgtcggg cgtgccgaag cgctcgacct tgctggtctg ttgcagttgc gtgatccaat
      661 cgttcatcag ttgcgagcgc tggtcgtcgc tggtggcccc ggccacgacc ttgaggccga
      721 tgtagctgtt gatcgccacc acgtcgcgct ggtcggcata gaggtcggcg cgggtcaggc
      781 cgtagccctt gagcgccgtg tccaggtcgt ccatcgtctt accgttgcgc gtgatcagcg
      841 tctgcacaaa cgcgtcgata tcggcgtcgc tgacggtgat gcccgcgcgc gcggcgtcct
      901 gggcgcgcat gcggcggtcg atcaggctct cgacggtggc cgagcggctc agcgtctgct
      961 catcgccggt cagcagcgcg gtcagagcgc ggttgagcaa gatttcgcgg tccacctcct
     1021 gcacagtgat gttttcgccg ttgacgcgcg ccagcacgcc ggggggcggc tgggtgctcc
     1081 accaggacag ggcaaagaac ccggccacga ccagcgccgc gagaatgagc actccgccca
     1141 ggatcttctg ttgcagcttc acgcggcgct tgacgcgcac gccgccccag gcggcctcgg
     1201 cctccggcgc gcgccggccc gctttcgcgc ccgccgccgg atgcagcgcc gcctgctgat
     1261 cgacggcgcg ctgcccgcgt cggatttcga cattgaagcc gggttccgcc ggcccgttgg
     1321 aggaagatcg gttcatcgca ttagattccc agggtccagg tcggcaggaa ctgattgagc
     1381 cacgtcacac ggtccatcat cagcaacccg cccatgaaca agagcaacac cccgctcacc
     1441 agcgagatgg gattgagtcc cgcgaccagg gtgcggccgg ccagccgcag gtcaaaggag
     1501 cgcatcatca ccttgcgcag ccagatcgtc agttgcccgg ccagcagcgc ggccaggata
     1561 aacggaatgc ccaggcccag cgaataggcg atgaacaggg ggacggcctc cgtagtgcgc
     1621 tcctgcatcg ccagggtgta catcaggccc aggaaggggc cgacgcacgg cgtccagccg
     1681 atggcgaagc cggtgccgat cagcacggaa cgcacatagc cgaggttctt gctggggtgc
     1741 aactccaggc gccgctcgta gttcaggaac gggatctgga agacacccag cgtgtgcagc
     1801 ccgaagatca gcagcaggaa gccgaggacg atctggatcg gctggcggta ctcggccagc
     1861 cagtcgcgca cgacgcctgc actggcgccc agcagcacga agatgaagga gaacccggcg
     1921 acgaaagcga gggcgtgcaa aaagacccgc cagcgcgccg cctgcattcg cccccgctgg
     1981 atgctggtcc cgctcatata gccgagatag accggcacca gcggcagcac gcagggcgat
     2041 gcgaacgaca ggaaccccgc cagcacggcc acgaagatat tcgcgttact ttcaggcaca
     2101 ggctagacct cgtcggcgag aggaatgggc cgcgcgggca agggccacac ggcgagacca
     2161 cgggacaggc gcgccgtctc gcgcgcgatc aggtggatac agtttgcggc gcctcgtacg
     2221 gtggcaccgg ggccgcgggc acccagtaca cgctgcggct ctcctccacg gcccgcagca
     2281 cccagggata gaccaggcgc acgacggcga agccgaagat ggccccgctg atcacgcgca
     2341 accaccagtt caaggtgccg gcgctgtcgc ccaccgagaa gtcgccgaag cggcccccgg
     2401 tcagcgtgtc gaaccaggcg ttgtcctcgc gcaacccgaa catatgggtc aggccgtcca
     2461 cggcgatggg cacgagcagg accacgaaga cctgccagcg gatgggccgc agcatccagg
     2521 ccagccggcc cccgaacgcg cctcgccggg ccgccgcaaa gagcagcccg cccaggaaga
     2581 tcgtcgtgta gatggcgcta ttgcggtaac agtaggccac ctggtagccg aacaggttgc
     2641 ccgcccgctc cggcagttgg tggcaaaaga acatgtagat cgtgtagatt gggtcggcca
     2701 ggaaccacca gcccagggcg gcaaacaccg gggccagcca gggcagcgtc gagaagatgc
     2761 cgatggccgt gttcaccacc gccagccagt gggtggtcat accgttgccg aagcgctcga
     2821 aggctccgaa caccccagtc tgcgggcggg gcgggccgta ataatacggg tatggatagg
     2881 cttgcgccgg gacggttgcc cctgtcgatc catcccctgg caccgaggcg gcccctgctg
     2941 ccgggtccag gccgggacct gtcgccggcg cagtagcgcc cggcgcatcc gcaggcatgg
     3001 ccggagccgg agtcgcgcgc gcaggtccct gatcgagcgc ggcgcgcagg tgcgccacgt
     3061 cgagcgaggc cggggccggg atcggcgtcc cgccgtccac caccactacc gggatcacat
     3121 agcgataggc cgcgtacagg tcctgatcgt ccaggatgct gacctcgtcc accgtccagg
     3181 caggcgcatc gggccgctcg gcgatgacct gccgcaggta gtggcgcgcg tgatcgcaga
     3241 gcgggcagtt gggcttgctg tagagtgtga ttgtcggcat aagatcggtg ctttcgtgcc
     3301 gccggcgcgc gatcccgcat cctccagccg ccgcgggatc cggcggtaat agccctatta
     3361 tactcggcgg ttgcgcgccc cgtcaaacat gcccggttta gcagtggtca gtggtcggcg
     3421 gtcagtggtc agtacgacaa gggcggggcg ccccagaccg gcaaccgaga acggtcattc
     3481 cgagcgcagc gaagagtctc gtccgtgccg gccacaaaac gtgacccttc gctgcgcttg
     3541 gagtgataag ccatggcgtg ggcctgatcg ctgggcactc ggccctcggt atttggcgcg
     3601 cacccgcgtt cagcacagac caccgaccac cgaccaccga ccactgacca ctgccggcag
     3661 gtggcaggaa gccggcggcc tatgatacaa tacgttagag tacccgctcc aggttcttct
     3721 ggcgcaatta ccccacgggt ccaacccatc cgcccattcg caatgtagta gtcaaaggag
     3781 acttgttaat ggtatcgact gtcaagccca ccgccgagac gcagcagctg ccgggaccga
     3841 aagccgaagc cctggtagcg cgcgacgacc gcgtcatttc cccgtcgtac acgcgcagct
     3901 acccgtttgt catggatcac ggcaagggca gcgaggtctg ggatgtcgac ggccggcgct
     3961 ttatcgactt caccgccggg gtcgctgtgc tggtggcggg gcatgcccac ccggccatcg
     4021 tggaagccat cacgaagcag gcgcagaagt acatccacat ggcgggcacc gatttctacc
     4081 tgccggaagc agtggagctg gctgagaccc tggcccggat tacccccggc gattatgaga
     4141 agcaggtctt cttcaccaac tcgggcacgg aatcgaacga agcggcgatg aagctgtgcc
     4201 actggtacac cgggcgcccg gcgtttatca gcttcctggg cgcgttccat ggccgcacgt
     4261 acggcagcat gtcgctgggc agcagcaagg cgctgcaccg cgcgcgctac caaccgctgc
     4321 tgtcaggcat ctaccacgcg ccctatccga acccctatcg cccgccgttc gacgtgccca
     4381 ccgagcgcct ggggcgcgcg tgcgtggact acatcgaaca cacgatgatg aagcacctgg
     4441 tgcgcccgga tgaggtcgcc ggcatcttcg tcgagccgat ccagggcgag ggcggctatg
     4501 tggtgccgcc gggcgatttc ctgcccgccc tgcgcgccct gtgcgacaag tacgacatcc
     4561 cgctggtggc cgacgaagtg cagtcgggca tggggcgcac cggcaagatg ttcgccagcg
     4621 agcactgggg cgtggtgccc gacgtgatct ctatcgcgaa aggcatcggc ggcgggctgc
     4681 cgctcggcgc catcgtggcg cccaagcgta tgatgacctg gccgtcgggc gcgcacgcca
     4741 acaccttcgg cggcaacccg gtggcctgcg ccgccgcgct tgccacgatc aacctgctcg
     4801 aaaccgaact gctcgccaac accaacgagg tgggcgcgca gatgatggcc cggctacaga
     4861 cctggcctga tcgcttcccc atcgtcggcg aggtgcgcgg ccgcgggctg atgctgggca
     4921 tcgacctggt cgaagaccgc acgacgcgca ccccggccca cgacctgcgc gaccagattg
     4981 tggacgaagc cttccaccgc ggcctgctgc tgctgggggc cggcgccggg gccattcgcc
     5041 tctgcccgcc gctgatcctg acccaggacc tggccgacga ggggctgacc atcctcgaag
     5101 atctgctcgc cgcccatagc taagcgcggg ccgggcaggg caaaaaagga agccgtctca
     5161 taacgagacg gcttctctgt taccggacgc gaaagtatgg ggcgcgcata ggagta
//
LOCUS       DASUYC010000025         2440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_558351,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000025 DASUYC010000000
VERSION     DASUYC010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558351"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="VF276_00360"
     CDS             <1..884
                     /locus_tag="VF276_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03621 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX6605342.1"
                     /translation="PFRFGVVSSGVGRARDGWIDLARRAEALGYAALLMPDRPSLGGP
                     APIAALATAAGATTTLRVGSYVFANDYRHPVIVAREAATLDVLSGGRFDLGLGAGVGH
                     GEYEQLGLPFDPPGVRVSRLEEALRLIKQLFTEDRVSFSGRYYAVRDLPGLPKPVQQP
                     HPPIFIGSSGKRMLTFAAREAQMIAPTLKYTPQGPDPADVSLDEKVGWIRVAAGDRFA
                     AIELCQTRYDIAVSDSPAPLAQQAGGPPIPKRPLTIDQAVAALEDERARYGFSYIQIS
                     DTQLENFAPVVARLGGK"
     gene            1132..2403
                     /locus_tag="VF276_00365"
     CDS             1132..2403
                     /locus_tag="VF276_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605343.1"
                     /translation="MTTMKRRVLAGVSLLSVPALFVGMHFATATAAEEPIPFADPAFR
                     GVWARTDFPVANHTVNRTWFWGPSPNTPGLIEEVRDLQGGTRLVQYFDKSRMELNNPA
                     GNPNDPFYVTNGLLTVELISGRMQVGTNDYVTRYPACIPTTGDPGDTNAPTYWAMQKV
                     SNTTLGDHFADDRTGQGVTETIDHDGNVSNNPPMNVITGTVNAYFDTVTKHNIPKVFW
                     DFLNQTGPVYMNDTITNTQVITPWFYASGRPISEAYWTKATIAGQLTPVMIQMYERRA
                     LTYVPTNPDGWQVEMANIGQHYYDWRYKGAGNCGTGTPAATVQPTFAVTGTPGTAVPS
                     GTAEATGTAEPEGSPSPSETVMPGGTVTATTSPMATTTVQATTTAAATTTAGATTTAE
                     ATVTPSPSGSPEPVVTVPDPMLPTDTPTPAP"
BASE COUNT          429 a          844 c          796 g          371 t
ORIGIN      
        1 atccgtttcg ctttggcgtg gtgagcagcg gcgtgggccg cgcacgcgac ggttggatcg
       61 acctggcgcg gcgggccgag gcgctcggct acgcggcgct gctgatgccg gaccggccct
      121 cgctgggcgg gccggcgccg atcgcggccc tggcgacggc ggcgggggct acgaccacgc
      181 tgcgggtcgg cagctacgtc ttcgccaacg actaccggca cccggtgatc gtggcgcgcg
      241 aggccgccac gctcgatgtg ctgtcgggcg ggcgtttcga cctgggcctg ggcgccgggg
      301 tgggccacgg cgagtacgag caactgggcc tgcccttcga cccgcccggt gtgcgcgtgt
      361 cgcgcctcga ggaggcgttg cggctgatca agcaactctt taccgaggac cgggtctcgt
      421 tcagcgggcg ctattacgcg gtgcgggatc tgccgggcct gcccaagccg gtgcagcaac
      481 cccacccgcc catcttcatc ggcagcagcg gcaaacggat gctgaccttc gccgcgcggg
      541 aagcgcaaat gatcgcaccc acgctcaaat acacgcccca gggccccgac ccggcggatg
      601 tctcgctgga cgaaaaggtg ggctggatcc gcgtggcggc gggcgaccgg ttcgccgcga
      661 tcgagttgtg ccagacgcgc tacgatatcg ccgtgtccga cagccctgcg ccgctcgccc
      721 agcaagcagg cgggccgccc atccccaagc gcccgctgac catcgatcag gcggtcgcgg
      781 ccctggaaga cgagcgggcg cgctatggct tctcctacat ccagatttcc gatacgcagt
      841 tggagaactt cgcgcccgtg gtggcgcggt tggggggcaa atagcagggc tatgccgccg
      901 gcccgccagc cacgccccga tgctcaattg cagcgcaccg gcgggctgtt ttcgtcgcta
      961 ctagcggatg tggtacataa cttgcttgaa atttaggccg atagggagcc aggactgcgg
     1021 gtgtacttcc gcgctctcct ggtgcatgcc gcgacgaccc gtgctggcgc agcagttttg
     1081 ggagtcggcc aaatcccttt tcggcataac agcgcaagga ggaacgaaac tgtgactacc
     1141 atgaaacgac gcgtgctggc gggggtgtcc ctgctcagcg tgccggcttt gtttgtcggg
     1201 atgcactttg ccaccgccac cgccgccgag gaaccgatcc cgtttgccga cccggccttc
     1261 cggggcgtgt gggcgcgcac cgattttccc gtcgccaacc ataccgtcaa ccggacctgg
     1321 ttctggggcc cgtcaccgaa cacccccggc ttgatcgaag aggtgcgcga tttacagggc
     1381 gggacgcgcc tggtgcaata tttcgacaag agccgcatgg agttaaataa tccggcgggc
     1441 aaccccaacg atcctttcta cgtgaccaat ggcctgctca ctgtggaact gatttcgggg
     1501 cgcatgcagg tgggcaccaa cgactatgtg acccgctatc ccgcgtgcat ccccacgacg
     1561 ggggatcccg gcgacaccaa cgcgcccact tactgggcga tgcagaaggt cagcaatacg
     1621 accctgggcg accattttgc ggacgatcgc accggccagg gcgtgaccga gaccatcgac
     1681 catgacggca acgtgagcaa taacccgccc atgaacgtca tcaccgggac cgtgaacgcc
     1741 tacttcgata ccgtaaccaa gcacaacatc ccgaaggtct tctgggactt cctgaaccag
     1801 accggcccgg tctacatgaa cgacacgatc accaataccc aggtgatcac cccctggttc
     1861 tatgccagcg ggcgcccgat cagcgaagcc tactggacca aggccaccat cgccggtcag
     1921 ttgacgccgg taatgatcca gatgtacgag cggcgcgcgc tcacctacgt gccgaccaac
     1981 ccggacggct ggcaggtcga gatggcgaac atcggccagc actactatga ctggcgctac
     2041 aagggcgccg gcaattgtgg caccggcacc ccggcggcga ccgtccagcc gacgttcgct
     2101 gtgaccggca cgcccggtac tgccgtcccg agcggcaccg cggaggcaac cggcacagcc
     2161 gaaccggagg gcagcccgtc gccgagcgaa acggtgatgc cgggcggcac cgtcacggcg
     2221 accacctcgc cgatggcgac caccacggtg caggccacca ccacggcggc ggctacgacc
     2281 accgccgggg cgaccacgac ggcggaggcg acagtgactc cgtcgcccag cggcagcccg
     2341 gagccggtcg tgaccgtgcc cgatccgatg ctgccgaccg atacgccgac cccggcgccg
     2401 taacccggcg gcctcggcca gcgccacccc tctgcgcggg
//
LOCUS       DASUYC010000026         3801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1020102, whole genome shotgun sequence.
ACCESSION   DASUYC010000026 DASUYC010000000
VERSION     DASUYC010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020102"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..619
                     /locus_tag="VF276_00370"
     CDS             53..619
                     /locus_tag="VF276_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605344.1"
                     /translation="MIIDFGPKAGLLIRRVWIEELPDVRYPVEDVVEYSLEAAPKGWG
                     AMRSAAAEMVRPSCGPSSYALIGAKFLPAATGSLRIQVAISEAGGEKLETPANEEQAG
                     APIFHSTKFVGFPREFLRGVFDVAVDAADLLGSGLLRFDHAAHDSVGSSIMAFRWATQ
                     ATVRLLTINSNAISEDELRQLLVLDRDR"
     gene            complement(621..944)
                     /locus_tag="VF276_00375"
     CDS             complement(621..944)
                     /locus_tag="VF276_00375"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX6605345.1"
                     /translation="MNRIAATDRTFRRDGDRWIGKCLICNGPLAFDAATGVGANIEHI
                     VPRAQGGSNDLLNLGITHPHCNGEKGVHWDAPRRRRANPDRYAQLVARLLAERRRRWR
                     EPLES"
     gene            1108..1392
                     /locus_tag="VF276_00380"
     CDS             1108..1392
                     /locus_tag="VF276_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605346.1"
                     /translation="MTVAQLIVYLLLAAIVGLIAERLVGAGPWGLVGNIIVGLLGIWV
                     MLNVLHWRLPGDLEIEGVPILTGILGAILVDLLLSLLLRGLGPRRAWRRR"
     gene            complement(1457..3484)
                     /locus_tag="VF276_00385"
     CDS             complement(1457..3484)
                     /locus_tag="VF276_00385"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX6605347.1"
                     /translation="MDQCGFLTGRERATLRAVCDTLLPALEPPPGVTGTAQVAYWRRS
                     AADLDVAAAVEETLWLETTPEQQAQIRQALGLLVQPAALRLLTGSARPFPARSQAGRE
                     AVLRAWATSPLPLLRQAFQVFKRLACFHFYAHPPSAGSNPNATVLGYPGFELPPPAPP
                     ANPAHPLSPLTIDRDTVLDADVCVVGSGAGGGVAAALLAQAGRRVVVLEAGGWYTEAD
                     FTAEEESGWERLYMRRGALATDDAGMLILAGRTLGGGTTVNWTTCFRPPAALLAEWAA
                     RSGVADLQGADLQASYAAVEARMGIHIDDGPFNGNNAVVWNGAAALGLAPARNPRNIT
                     HCGTCGICHFGCRWGAKQGTMRTYLQDAYDAGAQIVPNCYADRVLLMGDRVVGVEARV
                     RRPVEERAPDAPAHRLTVRAPTVVVAGGALESPALLLRSGLRHPALGRNLYLHPATGV
                     IGRFDAPVEAWRGPMQDVYSNALADLTGDGYGVRFEAVPLYPGFAAITAPWTGGRAFR
                     AYLGGLAHYNALLVLTRDRVPGRVVLDSQGRPRLLYRPSAESRRLLLRGCQEGARLVL
                     AAGAREAQTLHTVPLRMRSMGGLDRFQAEVAAAGAAPNRMTIGSAHQMGTCRMDAHPA
                     RGVVDRRGAVHGVRGLYVCDTSIFPSASGVNPMLTVLGLAHWVVQRGLLAG"
     gene            complement(3487..>3801)
                     /locus_tag="VF276_00390"
     CDS             complement(3487..>3801)
                     /locus_tag="VF276_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605348.1"
                     /translation="ETPVAPARPAAVAPAAAKEAPAPATPASASNEAPARDTIESLNI
                     RQRRLLTLLRDRGGITRVEYESFFHVGTRTAKRDLKGLLDAGLIEHRGSSTASYYVLG
                     RS"
BASE COUNT          567 a         1240 c         1373 g          621 t
ORIGIN      
        1 cgccgcctgg cttgatgcat gatctccccc gatccagtat ggagttgcct cgatgataat
       61 cgattttggg ccaaaagctg gactactcat acgccgtgtt tggatagagg aactgcccga
      121 tgtgcgctat cctgtggaag atgttgtgga atatagcttg gaagcagctc ctaaagggtg
      181 gggtgccatg cggtccgccg cggcggaaat ggtccggccc agttgcgggc catcttccta
      241 tgcactgatt ggcgcgaaat ttctcccggc tgccaccgga tcactccgca tccaggtagc
      301 gatatctgag gccggcggcg aaaagctgga gactcccgct aacgaagaac aagcgggcgc
      361 accgattttc cattccacca aatttgtggg ctttccgcgg gagtttctaa gaggcgtgtt
      421 tgatgtcgcc gtagatgccg cggatctttt ggggtccggc ttgcttcgct ttgatcacgc
      481 tgctcatgac tcggtaggat cttccattat ggcgttcaga tgggctacgc aagcgacagt
      541 taggctgctt accatcaaca gcaacgccat ctccgaagac gagttgaggc agttactggt
      601 tttggacaga gatcggtaga ctaggattcc agtggttcgc gccagcggcg gcggcgttcg
      661 gccagcaggc gcgcgacgag ttgggcgtag cggtcggggt tggcgcggcg gcggcgcggc
      721 gcgtcccagt ggacgccctt ctcgccgttg cagtggggat gggtgatgcc caggttgagc
      781 aggtcgttgc tgccgccctg ggcacggggc acgatgtgct cgatgttggc gcccacgccg
      841 gtggcggcgt cgaaggccag tggcccgttg cagatcaggc acttgccgat ccaacggtcg
      901 ccgtcgcgcc ggaaggtgcg gtcggtggcc gcgatgcgat tcatccaatc cgtggttttc
      961 atcgatcaga gtacgcgccg ggccgctggg acggatcgcc gggcagaggg cgaacaaagg
     1021 ttcggctacc tggcatggtt gttgctttta gagcagcagg cgcagtgtgg ttgcgccgcg
     1081 tgtaatcctt agtggagggc acacaccatg acagtagcac agttgatcgt ctatttactg
     1141 ctggcggcaa tcgtcggcct gatcgccgag cggttggtcg gcgccggtcc ctggggtctc
     1201 gtcgggaata tcatcgtggg cttgctcggc atctgggtga tgctgaacgt gttacactgg
     1261 cggttgcccg gcgacctcga aatcgaaggc gtaccgatcc tcaccggcat cctcggcgcg
     1321 atccttgtcg atctgctgtt gagtctcttg ttgcgcggcc tgggtccgcg gcgcgcctgg
     1381 cgccgccgct gatccggcat cccaacttcc ctcatgcggg ccgccccgaa aggggcggcc
     1441 tttttggtgc cccacgctac ccggccagca gaccccgttg cacgacccaa tgggccaggc
     1501 cgagcacggt cagcatcgga ttcacgccgg acgccgaggg gaagatgctg gtgtcgcaga
     1561 cgtagaggcc gcgcacgccg tggacagccc cgcgcctgtc caccacgccg cgggccgggt
     1621 gagcgtccat gcggcaggtg cccatctgat gggcgctgcc aatggtcatg cggttgggcg
     1681 cggcgcccgc ggcggccact tccgcctgga agcggtccag cccgcccatg ctccgcatgc
     1741 gcaacgggac tgtgtgcagc gtctgcgctt cgcgggcgcc cgccgccagc accaggcgcg
     1801 cgccttcctg gcagccgcgc agcagcagcc gccggctctc ggcggagggg cgatagagca
     1861 gccggggccg gccctggctg tcgagcacca cccggcccgg cacccggtcg cgggtcagca
     1921 ccagcagggc gttgtagtgg gccagcccgc ccaggtaggc gcggaaggcg cggcccccgg
     1981 tccacggcgc ggtaatggcc gcgaagccgg ggtagagcgg caccgcctcg aagcgcacgc
     2041 cgtagccgtc gccggtcaga tcggccaggg cgttcgagta gacatcctgc atggggccgc
     2101 gccaggcttc gaccggcgcg tcaaatcgcc cgatcacgcc cgtggccggg tgcaggtaga
     2161 ggttgcggcc aagggccgga tgccgcagcc ccgagcgcag cagcaacgcg ggcgactcca
     2221 gcgcgccacc cgcgaccacc actgttggcg cacgcacggt gagccggtgg gccggggcat
     2281 cgggggcgcg ttcctcgacc gggcggcgga cgcgcgcctc cactcccacc acgcggtcgc
     2341 ccatcagcag cacgcgatcg gcgtagcagt tgggcacgat ctgagcgccc gcgtcgtagg
     2401 cgtcttgcaa gtaggtgcgc atggtgccct gcttggcgcc ccagcggcaa ccgaaatggc
     2461 agatgccgca ggtgccgcag tgggtgatgt tgcgcgggtt gcgggcgggg gcgaggccca
     2521 gcgccgccgc gccgttccag acgacggcgt tgttgccgtt gaaaggcccg tcgtcgatgt
     2581 ggatacccat gcgcgcctcg acggcggcgt agctcgcctg gagatcggcg ccctgcagat
     2641 cagccacgcc cgaccgcgcc gcccattcgg ccagcagcgc agcgggcggg cggaagcagg
     2701 tggtccagtt gaccgtggtg ccgccgccca gcgtgcgccc ggccaggatc agcatcccgg
     2761 cgtcgtcggt ggcgagcgcg ccccggcgca tatagagccg ttcccagccg ctttcctcct
     2821 cggcggtgaa atcggcctcg gtgtaccacc cgcccgcttc gagcacgacc acgcgccgcc
     2881 cggcctgggc cagcagggcc gccgccaccc cgcccccggc ccccgacccg accacgcaca
     2941 cgtcggcgtc gagtacggtg tcgcggtcaa ttgtcaacgg gcttagcggg tgggccgggt
     3001 tggcgggcgg cgcgggcggc ggtagctcga agccggggta gccgaggacc gtggcgttgg
     3061 ggttagaccc ggcgctcggc gggtgggcat agaagtggaa gcaggccagg cgcttgaaga
     3121 cctggaaagc ctggcgcaac aggggcagcg ggctggtggc ccaggcgcgc aacaccgcct
     3181 cgcgtccggc ctggctgcgg gccgggaacg gccgcgccga gccggtcagc aggcgcaggg
     3241 ccgcaggctg gaccagcaag cccagcgcct gccggatctg cgcctgctgc tcgggcgtgg
     3301 tttcgagcca caacgtctct tctacggcgg cggccacgtc caggtccgcg gcggagcggc
     3361 gccagtaggc gacctgggcc gtgccggtca cgccgggcgg cggctctaac gcgggaagca
     3421 atgtgtcgca gacggcgcgc agggtggcgc gctcacgccc ggtcaggaag ccgcactgat
     3481 ccattgttag cttcgcccca gcacgtaata gctggccgtc gatgagccgc gatgctcgat
     3541 cagccctgcg tcgaggagtc ccttcaagtc ccgcttggcc gtgcgcgtgc cgacatggaa
     3601 gaaggactcg tattccacgc gggtgatgcc gccgcggtcg cgcagcaggg tcagcaggcg
     3661 gcgctggcgg atgttcaggc tctcgatggt gtcgcgcgcc ggggcctcgt tagaggcaga
     3721 cgcgggcgtg gcgggcgccg gtgcctcctt cgccgccgcg ggtgcgacgg cggcaggccg
     3781 ggccggcgcg accggcgtct c
//
LOCUS       DASUYC010000027         5364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1482653, whole genome shotgun sequence.
ACCESSION   DASUYC010000027 DASUYC010000000
VERSION     DASUYC010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5364
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1482653"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            466..1233
                     /locus_tag="VF276_00395"
     CDS             466..1233
                     /locus_tag="VF276_00395"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6605349.1"
                     /translation="MHIRLLGVHQGESKTTKFISILVDGVLAIDAGSLTSGLTFDEQQ
                     KLNTILVTHCHYDHIKDIPMIAFNNMHTSQILVYCQADTREALESHIMNQSIWPKMYD
                     IPRPEAPSIRFCEVEAGRRFHINGYSVLPVPMKHAVPTVGYAVHHNGKSFFFTSDTGG
                     DCHQAWLQMQPDLIISEATLPNRLIADADAYGHLTPSKIERELRDFRALHGYVPHVIA
                     VHIDPKHEAEVRAEIAEVAARLDAPIEVGYEGMCIDL"
     gene            1218..2387
                     /locus_tag="VF276_00400"
     CDS             1218..2387
                     /locus_tag="VF276_00400"
                     /inference="COORDINATES: protein motif:HMM:NF014041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF84 family protein"
                     /protein_id="HEX6605350.1"
                     /translation="MHRSVTESAFRPAAVPEDGVSFAPRRIAVGSANPGKIAAVRQAV
                     ARYWPQAGVEGIDVQSGVPDQPRNDAEGARGAVLRAVAARHALDADLGIGLEGSSDEH
                     PWGMYTVAWVAVVDRAGRVGLASTGRCPLPASVAAGIRMGGELGPLVDDLLGEANTKH
                     RGGASAAFTAGHAGRIDSLAGGVVYACAPFLTPAYFGPAAMGHVDDVLARLAEARPLR
                     TAYTLVFLQRPDGRVLMLHRTRPPNAGRLNGVGGGIEAGEDVLHAAAREVREEVGLDV
                     QPRLALALTLWEAGRLAQGAPPILLYVCHAGITAEAAAQVPAACPEGALSWVDPQALA
                     ALDVVPNLRVLLPLLPAPEAGAGALAGTLWYDAGWQGGRYLIHTPGGPRSGPIPP"
     gene            2894..4789
                     /locus_tag="VF276_00405"
     CDS             2894..4789
                     /locus_tag="VF276_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HEX6605351.1"
                     /translation="MDLIRKLEEYRAQEQALAWEGTFADYFEIVKQQPQVAQLSHARV
                     FNMIMSEGVEVGKRGETRYNFFNQEIFGLDRPLQQIVEYFNSAAQRLEVRKRILLLMG
                     PVGGGKSTIVSMLKHGLEQYSRTERGAVYAIATCPMHEEPLHLIPPSLRSDFEREYGI
                     YIEGDLCPRCRFDLEHTWEGHIERVPVRRVAMSEKQRIGIGTFTPSDPKSQDVSELVG
                     SIDLSTIGEVGSESDPRAYRFDGELNIANRGIMEFIEILKSDERFLYVLLTLSQEQNI
                     KTGRFSMIYADEVIVSHTNESEYTTFAANKKSEALQDRVILVRVPYNLRVSDEVRIYE
                     KLLKQSSLQNVHIAPHTLRVASTFAVLSRLEPSKKAGMSLMKKLRLYDGEDVEDFKIK
                     DVKELQEESVREGMEGISPRYIINRLSSALVREGITCINPIDALRALRDGLEQHTSIT
                     REQRDHYLNLIAEARKEYDELAKKEVQRAFVYSYEEAARTLLNNYLDNVEAYCNKQKL
                     RDPITEEEIDPDEGLMRAIEEQIGVSENAKKAFREEILIRISSLARRGQSFDYTSHER
                     LKEAIEKKLFADLRDVVKITTSARTPDPEQLRKINEVVDRLVRDHGYCTVCANALLTY
                     VGSLLNR"
     gene            4928..>5364
                     /locus_tag="VF276_00410"
     CDS             4928..>5364
                     /locus_tag="VF276_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HEX6605352.1"
                     /translation="MANDEQSPEFRLPLMSIGQHDWSLHRKGPADQQRHQEKIKEAVR
                     KNLGQIVSEESIILSDGQKTIKVPIRTLDEYRFRFDYSSGKHTGAGDGDSQVGDVIGQ
                     AGNPRGKGKQGQGPGEAGDAPGADYYEAEISIDELAAMIFQDLG"
BASE COUNT         1016 a         1816 c         1695 g          837 t
ORIGIN      
        1 ggaacgctac gaggatgcgc tggctgccta cagccaggcc atcgagatcg acccgcaatg
       61 ggcgcccgcg tataagggcc gcagcgcagc ctacgccgcg ctgggcgaca cccggcgggc
      121 gcgcacggac ctggcccgcg cccgcaaact cgatcccagc atcgagtgat cccggtgttt
      181 gtggccgggg cgggccggtg agcaacccgc ccgcctcggt gcggccccga tcgacctcga
      241 ccccggacct ttatgagata aaggtactag cggggcctac ttgtcaaccc gatcaaagta
      301 gtgctataat cggctctatg atgccggggc gcggggggcg ccgggcacat ccctaagcct
      361 cacggcggac ctttcacgcc tgaacccggc ctgactgctt gttggtcaag cagcgcatat
      421 tcggcaggcc actcgcagta ccgcccggaa agtcaggccg cacctatgca tatccgcctc
      481 ctcggcgtgc atcaaggcga gagcaagaca accaagttca tctccattct ggtggacggc
      541 gtactcgcca ttgatgcggg cagcctcacc tcggggctga cctttgacga gcagcaaaag
      601 ctcaacacca tcctcgtcac ccactgccac tacgatcaca tcaaagacat tcccatgatc
      661 gccttcaaca atatgcacac cagccagatc ctggtctatt gtcaggcgga cacacgggaa
      721 gcgctggaat cgcatatcat gaaccagagc atctggccca agatgtacga tattccgcgc
      781 ccggaggcgc ccagcatccg cttctgcgag gttgaggcgg gccgccggtt ccacatcaac
      841 ggctacagtg tgctgccggt gcccatgaag cacgccgtgc ccaccgtggg ctatgcggtg
      901 caccacaacg gcaaaagctt cttcttcacc tcggacaccg gcggcgattg ccaccaggcg
      961 tggctccaga tgcagcccga cctgatcatc agcgaggcga cgctgcccaa ccgcctcatc
     1021 gccgacgccg acgcctacgg ccacctcacc ccgtcgaaga tcgagcgcga actgcgcgac
     1081 ttccgcgccc tgcatggcta cgtgccccat gtcatcgccg tgcatatcga ccccaagcac
     1141 gaggccgaag tgcgtgccga gatcgccgag gtcgctgccc ggctcgacgc gcccatcgag
     1201 gtcggctacg agggcatgtg catcgatctg tgaccgagtc cgcgttccgc cccgccgccg
     1261 tccccgagga cggcgtctct tttgccccgc gccgcatcgc cgtgggcagc gccaaccccg
     1321 gcaagatcgc cgccgtgcgc caggccgtag cgcgctactg gccgcaggcc ggcgtcgaag
     1381 gcatcgacgt gcagtcgggc gtgcctgacc agccgcgcaa cgacgccgag ggggcgcggg
     1441 gcgcggtgct gcgtgcggtt gccgcccggc acgcactgga tgccgacctg ggcatcggcc
     1501 tcgaaggtag cagcgacgag catccctggg gcatgtacac cgtggcctgg gtggcggtgg
     1561 tggaccgcgc cgggcgggtg gggctggcca gcaccgggcg ctgccccttg cccgcttccg
     1621 tcgccgcggg catccgcatg ggcggcgaac tcggcccgct ggtcgacgat ttgctcggcg
     1681 aggccaacac caagcaccgc ggcggggcca gcgcggcttt cacggcgggc catgccgggc
     1741 gcatcgactc gctggcgggc ggtgtcgtct acgcctgcgc gcccttcctc accccggcct
     1801 acttcggccc ggcggccatg ggccacgtgg acgacgtgtt ggcccggctg gccgaggcgc
     1861 ggcccctgcg caccgcctat acgctggtct tcctgcagcg ccccgacggc cgcgtgctca
     1921 tgctgcaccg cacccgcccg cccaacgccg ggcgcctgaa cggcgtcggc ggcggcattg
     1981 aagcgggcga agatgtcctg cacgccgccg cccgtgaggt gcgcgaagaa gtgggcctgg
     2041 atgtgcagcc gcggctggcc ctggcgctga ccctctggga agccgggcgc ctggcgcagg
     2101 gcgcgccgcc gatcctgctg tacgtctgcc acgccggcat cacggcggag gccgccgcgc
     2161 aggtgcccgc cgcgtgcccg gaaggcgccc tgtcctgggt cgatccccag gcgctcgccg
     2221 cgctcgacgt ggtgcccaac ctgcgcgtgt tgctgccgtt gctgcctgcg ccggaggcgg
     2281 gggccggggc gctggcgggc acgctctggt acgacgccgg ctggcagggc ggccgttacc
     2341 tgatccacac accgggtggc ccgcgcagcg gccccattcc tccgtgagcg ggccgggcgc
     2401 cgggggccaa ttcctatagg gattgtagga ctctttggta gcttaatacc cgtatcgtcc
     2461 tcttgccctt gcgcgttgtt tatattagaa tagtacaaag ggcagcaggg gccgcccggt
     2521 caggacaggc ccgccgcagc cgcaacggcg cggataggtc tggctgaaaa cctgtctctg
     2581 cgatactggg cgtccccagg gtagcgtgtg tctcacatgc cgctctgggg acgttgtgta
     2641 tttactgccg cgcccacccg tgtgttccag ctccgtcaag cccactcggg ccggacaacc
     2701 cggtccccta ccccaggcta ccaggcgttt ccgccggtcg cctcggcgac aaaagcaacc
     2761 aacctgcccg gtcagccgta tttatagtaa gacacctgtt ctatcccagg caggatggta
     2821 gccataccca agctccgatc tgataccgat ctcgtaccgt agcagccggg caggaaaggg
     2881 aaggtgatcc gctatggatc tgattcgcaa actcgaagag tatcgcgcac aggaacaggc
     2941 actcgcctgg gagggcacct ttgccgatta cttcgagatc gtcaagcaac aaccgcaggt
     3001 ggcccagctc tcgcacgccc gcgtgttcaa catgatcatg agcgagggag tggaagtggg
     3061 gaagcgcggc gaaacgcgct acaacttctt caaccaggag atcttcggcc tcgaccgccc
     3121 gctccagcag atcgtggagt actttaactc ggcggcccaa cggctggagg tgcgcaagcg
     3181 catcttgctg ctgatggggc cggtcggcgg cggcaagtcg accatcgtca gcatgctcaa
     3241 gcatggcctg gagcaataca gccgcaccga gcgcggcgcg gtctacgcca ttgccacctg
     3301 ccccatgcac gaggaaccgc tccacctgat cccgcccagc ctgcgctccg acttcgagcg
     3361 cgagtacggc atctacatcg agggcgatct ctgcccgcgc tgccgcttcg atctggagca
     3421 cacctgggag gggcacatcg agcgcgtgcc ggtgcggcgc gtcgccatga gcgaaaagca
     3481 gcgtatcggc atcggcacgt tcacgccgtc ggaccccaag agccaggatg tttccgaact
     3541 ggtcggctcc atcgatctct cgaccatcgg cgaagtcggc tcggagagcg acccacgggc
     3601 gtatcgcttc gacggcgaac tgaacatcgc caaccgcgga attatggaat tcatcgaaat
     3661 cctgaagtcg gacgagcgct tcctctatgt tctgctcaca ctgtcgcagg agcagaacat
     3721 caagaccggg cgcttcagca tgatctacgc ggacgaggtc atcgtctcgc acaccaacga
     3781 gagcgagtac acgaccttcg ccgccaacaa gaagtcggag gcgttgcagg accgggtcat
     3841 cctggtgcgt gtgccgtata acctgcgcgt cagcgacgag gtacgcatct acgagaagct
     3901 gctcaaacaa agctcgctgc aaaacgtgca tatcgccccg cacaccctgc gcgtcgcctc
     3961 gaccttcgcc gtgctgtcgc ggctggagcc gtccaagaag gccggcatga gcctgatgaa
     4021 gaagctgcgc ctctacgacg gcgaggacgt ggaagacttc aagatcaagg atgtcaagga
     4081 actgcaggaa gagtcggtgc gcgagggcat ggagggcatt tcgccgcgct acatcatcaa
     4141 ccggctgtcg agcgcgctcg tgcgcgaggg catcacctgc atcaacccca tcgacgccct
     4201 gcgcgccctg cgcgacggcc tggagcagca caccagcatc acccgcgagc agcgcgacca
     4261 ttacctgaac ctgatcgccg aagcgcgcaa agaatatgac gagctggcga agaaggaagt
     4321 gcagcgcgcg ttcgtctatt cctacgagga agccgcgcgc acgctgctca acaactatct
     4381 ggataacgtc gaagcctact gcaacaagca gaagctgcgc gacccgatca ccgaggagga
     4441 gatcgatccc gacgagggcc tgatgcgcgc catcgaggag cagatcggcg tctcggagaa
     4501 tgccaagaag gctttccgcg aggagatcct gatccgcatc agcagcctgg cgcgccgcgg
     4561 ccagagcttc gattacacca gccacgagcg gctgaaggaa gccatcgaga agaagctgtt
     4621 tgccgacctg cgcgacgtgg tcaagatcac cacctcggcc cgcacgccgg atccggagca
     4681 actgcgcaag atcaacgaag tcgtcgaccg cctggtgcgc gaccacggct actgcacggt
     4741 ctgcgcgaac gcgctcctga cctacgtcgg ctcgctgctg aaccggtagt gacgagtggt
     4801 cagtggtcag tgatcagtga tcagttgaag tgcagatcct gagtctaagt gccgggcgaa
     4861 tccatccggc acttagcatt actgaccact gaccaccggc cactgaccac tcgcaaggac
     4921 gggcaccatg gccaatgacg agcaatcccc cgaattccgc ctgccgctga tgtcgatcgg
     4981 gcagcatgac tggtcgctgc accgcaaggg gccggccgac cagcagcgcc accaggagaa
     5041 aatcaaggag gcggtgcgca agaacctcgg ccagatcgtc agcgaggaaa gcatcatcct
     5101 gtccgacggg cagaagacga tcaaggtgcc gatccgtacg ctcgacgagt atcgcttccg
     5161 cttcgactac tccagcggca agcacacggg cgccggcgac ggcgactcgc aggtgggcga
     5221 cgtgatcggc caggccggca acccgcgcgg caaaggcaaa cagggccagg ggccgggcga
     5281 agccggcgac gcgcccggcg ccgattacta cgaggccgag atcagcatcg acgaactggc
     5341 cgccatgatc ttccaggacc tggg
//
LOCUS       DASUYC010000028         2421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_817315,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000028 DASUYC010000000
VERSION     DASUYC010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817315"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VF276_00415"
     CDS             <1..300
                     /locus_tag="VF276_00415"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEX6605353.1"
                     /translation="YYLSIVRRAGRQGYPRHGTQSPREYSAGLAPQLPQAHEDLDRLT
                     QEFIEARYSRHAIAPTEATQAQSHWQQVKAALQRARDQVTGAKPPPGPSDGTRTS"
     gene            323..1090
                     /locus_tag="VF276_00420"
     CDS             323..1090
                     /locus_tag="VF276_00420"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6605354.1"
                     /translation="MPHETDVVKAANSAFWDGAAARWRQLDLPAPALLQDWVGHLACA
                     PGALLLDAGCGVGRWSVPVAALGYRVSGVDLSPVMIREAQAKARRFDLAPERIQFQVG
                     DVEHLPFPDARFDGIICLNVLDFVPVPGSALLELRRVLKPGARMLVATLGAYSPIKYE
                     HWRRFLPANTVPAIANQILPWEMEAVLQELGWEIIHQQPDFEPSAAGQPNRYDEAWSR
                     QVSDRILQQTVASKWLFVARKSADQSAPEMESGRRER"
     gene            complement(1014..1724)
                     /locus_tag="VF276_00425"
     CDS             complement(1014..1724)
                     /locus_tag="VF276_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX6605355.1"
                     /translation="MINDRRAFILQNTRLQRPPFVPELQLHLADEITPLWHMTEAELG
                     ARGVPPPFWAFAWAGGLALARYLLDHPAEVAGKQVVDFATGSGLCALAALKAGAASAL
                     AADIDPFCEAAVALNAEANELRVAYTGQDLLAAAPLPADVILAGDICYEQPLAARVLA
                     WLQAAHARGTRVLIGDPGRSYFPRDELIQLAAYEVPTTRELEDSVVKAAGVFTFPVAP
                     TPSREQTDQQTSGRQTAT"
     gene            complement(1740..>2421)
                     /locus_tag="VF276_00430"
     CDS             complement(1740..>2421)
                     /locus_tag="VF276_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6605356.1"
                     /translation="GPLLTAAYNFLVLAGLYVVHGLTVMLTIFASVDAVSGEITSHTI
                     QTIVTKPVPRWQVLMGKYLGYAAMLLVYLGLLASGVLVATNMLVGYVPPNAVAGIGLI
                     LLEALVLLSLSLLGGTRLSTLTNGVALFMLYGLTFIGTWVQQIGAVLQSETAVRIGEV
                     SSWVLPVEALWRQAAFVLQPPNVLPNNFVSPFGALATPNPSIVYYALVYAAVAVGLAL
                     FSFSRRDL"
BASE COUNT          432 a          817 c          830 g          342 t
ORIGIN      
        1 tactacctga gcatcgtgcg ccgggcgggg cggcagggct atccgcgcca cggcacccag
       61 tcgccgcgcg agtacagtgc cgggctggcc ccgcaactgc cgcaggcgca cgaggatctg
      121 gaccggctca cgcaggagtt catcgaagcc cgctacagcc gccacgccat cgcgccgacc
      181 gaggcgaccc aggcacaatc ccactggcag caggtgaagg cggcgttgca gcgggcgcgc
      241 gaccaggtga ccggcgcaaa accgccgccc ggcccctcgg acggaacccg cacaagctag
      301 cccaacaacg caggaggcca ccatgccgca cgagaccgat gttgtcaaag ccgccaatag
      361 cgcattctgg gacggcgctg ccgcccggtg gcggcagctt gacctgcccg cgccggctct
      421 gttgcaggat tgggtgggcc acctggcctg cgcccccggc gcgctgctgc tcgatgccgg
      481 ctgcggggtg ggccggtgga gtgtgcccgt cgcagcgctg ggctatcgcg tgtccggcgt
      541 ggacctatct ccggtgatga tccgggaagc ccaggcgaag gcccgccgtt tcgatctagc
      601 gcccgagcgg attcaatttc aggtgggcga cgtggaacac ctgccctttc ccgatgcgcg
      661 gttcgacggc atcatctgcc tgaacgtgct ggacttcgtg ccggtgccgg ggagcgccct
      721 gctggagttg cggcgggtgc tgaagccggg cgcgcggatg ctggtggcga cgctgggcgc
      781 ctactcgccc ataaaatacg agcactggcg gcgctttctc cccgcgaaca ccgtacccgc
      841 catcgcgaac cagattttgc cgtgggaaat ggaagcagta ttgcaggagt tgggctggga
      901 aatcatccac cagcaacccg atttcgagcc gtcggcggca gggcaaccga accgctacga
      961 cgaagcctgg agccggcagg tcagcgaccg catcctgcaa cagaccgtgg cctctaagtg
     1021 gctgtttgtc gcccggaagt ctgctgatca gtctgctccc gagatggagt cggggcgacg
     1081 ggaaaggtaa agacgcccgc cgccttcacc acgctgtcct ccagttcccg cgtcgtcggc
     1141 acttcatagg cggccagttg gatcagttcg tcgcgcggga aatagctgcg gcccggatcg
     1201 ccaatgagca cgcgggtgcc gcgcgcgtgg gccgcctgca accaggccag cacgcgagcg
     1261 gcaaggggtt gctcatagca gatatcgccc gccaggatca catcggcagg cagcggagcg
     1321 gcggcgagca gatcctggcc cgtataggcg acgcgcagct cgttggcttc ggcgttgagc
     1381 gccacggcgg cctcgcagaa ggggtcgatg tcggcggcca gggcggacgc ggcccccgct
     1441 ttgagggccg ccagggcaca gaggcccgac cccgtggcaa aatcgaccac ctgcttgccc
     1501 gccacctctg ccgggtggtc gagcaggtag cgcgcgaggg ccaggccgcc cgcccaggcg
     1561 aaggcccaga acggcggcgg cacgccccgc gcccccagtt cggcctcggt catgtgccac
     1621 agcggcgtga tctcatcggc caggtgcagt tgcagttcgg gcacgaacgg cgggcgctgc
     1681 aagcgggtgt tttgcaggat gaatgcgcgc ctgtcgttga tcatcgtccc accgatgccc
     1741 tataaatccc ggcggctgaa cgagaacagc gccagcccca ccgccaccgc cgcgtagacc
     1801 agcgcgtagt agacaatgct gggattgggc gtggcgagag caccgaacgg cgagacgaag
     1861 ttgttaggca gcacgttggg cggctggagc acgaaggcgg cctgccgcca cagcgcctcg
     1921 accggcagca cccaactgct gacctcgccg atgcgcacgg ccgtctccga ctgcaacacg
     1981 gcgccgatct gctggaccca ggtgccgatg aaggtcaggc cgtagagcat gaacagggcg
     2041 acgccgttgg tcagcgtgga gagccgcgtg ccgccgagca gcgagagcga cagcagcacc
     2101 agggcttcga gcaggatcag cccgatcccg gccacggcgt tcggcggcac gtagccgacc
     2161 agcatattgg tcgccaccag cacgccactc gccagcaggc ccaggtagac gagcaacatg
     2221 gccgcgtagc ccaggtattt gcccatcagc acctgccagc ggggcaccgg cttggtgacg
     2281 atggtctgga tggtgtgcga ggtgatctcg cccgacacgg catcgaccga cgcgaagatg
     2341 gtcagcatca ccgtcagccc atggaccacg tacagcccgg ccagcaccag gaagttatag
     2401 gccgccgtca gcagcggccc c
//
LOCUS       DASUYC010000029         2055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_33583,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000029 DASUYC010000000
VERSION     DASUYC010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33583"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VF276_00435"
     CDS             complement(<1..192)
                     /locus_tag="VF276_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HEX6605357.1"
                     /translation="MRVIAGSARGLRLKAPEGRGTRPMTDRVKESLFGTLASLGYPQE
                     GDRVLDLYAGTGSLGIEALS"
     gene            complement(202..>2055)
                     /gene="recG"
                     /locus_tag="VF276_00440"
     CDS             complement(202..>2055)
                     /gene="recG"
                     /locus_tag="VF276_00440"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEX6605358.1"
                     /translation="GLLGLPDALRQVHFPADWGTLNQGRRRLAFDEFFLIQLGMLQRK
                     QHWQHDQPGLAFRVRPDILNSFRETLPFQLTGAQQRVLEEITADMERDLPMARLVQGD
                     VGSGKTVVAATALLAAIADGAQGALMAPTEILAEQHYRGLSKLFKNFWLPVGDANPKL
                     AEVAARLAAAKAEAEEAARQAQEDAAAAAAAADEPGADGTGKRKRKATVDSRTYAANE
                     TIVEASVELSPALAALKELLGKLDKSQNTGLQPLVESLEPPPGYRPLNIALLTGGMRK
                     RERDKIYKDLAEGTCDLVIGTHALITEGLQYAKLGLVVIDEQHRFGVAQREKLRQKAA
                     EIGVLADTLVMTATPIPRSLALTIYGDLDISIIDEMPPGRQVIKTRWIDDSRREQAYN
                     FVRREIDAGRQAFIICPLVEESDKIEAKSAVAEHERLQTEIFPDYRLALLHGRMKAAE
                     KEQIMAAFRRQEYDILVSTSVVEVGIDIPNATLMLVEGAERFGLSQLHQFRGRVGRGE
                     HQSYCILVSDTALSTSMQRLRVIEENHDGFRLAQEDLDIRGPGAFFGTRQSGAFDLRV
                     ARISDLETLQLARREAEYLFGQDPNLALPEHEPVRRQLEAFWELANSAPEA"
BASE COUNT          306 a          698 c          681 g          370 t
ORIGIN      
        1 cgatagcgcc tcgatgccca gcgagccggt cccggcatag aggtcgagca cgcggtcgcc
       61 ttcctgcggg tagcccaggc tggcgagggt gccgaacagc gactccttga cccggtcggt
      121 catggggcgg gtgccgcgcc cttccggcgc cttgagccgc agcccgcgag cgctgcccgc
      181 aatcacgcgc ataaggcggg cctacgcttc cggcgcactg ttcgccagtt cccagaaggc
      241 ttcgagttgc cgccgcacgg gttcatgctc cggcaaggcc aggttcgggt cctggccgaa
      301 caggtattcg gcttcgcgac gcgccagttg caacgtctct agatcgctga ttcgggccac
      361 ccgcaggtca aaggcaccgc tctgccgcgt gccaaagaac gcgcctggtc cgcgtatgtc
      421 gagatcttcc tgggctagtc ggaagccgtc gtggttctcc tcgatcacgc gcaagcgctg
      481 catcgacgtg ctgagcgccg tgtccgacac gagaatgcaa tatgactggt gctcgccgcg
      541 gcccacgcgc ccgcggaact ggtgcaactg gctcaggccg aagcgctcgg ccccttcgac
      601 cagcatcagc gtcgcgttgg ggatgtcgat gccgacctcg accaccgacg tggagacgag
      661 gatgtcgtac tcctggcggc ggaaggcggc catgatctgc tctttctctg ccgccttcat
      721 gcgtccgtgc agcagcgcca gccggtagtc ggggaagatt tcggtttgca gccgctcgtg
      781 ctcggcaacc gcggatttgg cctcgatctt gtccgactct tcgaccagcg ggcagatgat
      841 gaacgcctgc cgcccggcgt cgatttcgcg gcgcacgaag ttgtaggcct gttcgcggcg
      901 gctgtcatcg atccagcgcg tcttgatcac ctggcggccc ggcggcatct cgtcgataat
      961 cgagatgtcg aggtcgccgt agatggtcag ggccagcgag cgcgggatgg gcgtggcggt
     1021 catgaccagc gtatcggcca gcacgccaat ctcggcggcc ttctggcgca gcttctcgcg
     1081 ctgggcgacg ccgaagcggt gctgctcgtc gatcacgacc aggcccagct tggcgtattg
     1141 caacccctcc gtgatcaacg cgtgggtgcc gatcaccagg tcgcaggtgc cctcggccag
     1201 gtccttgtag atcttatcgc gctcgcgttt gcgcatcccg ccggtcagca gcgcgatgtt
     1261 gagcggccgg tagccggggg gcggctccaa cgactcgacc agcggctgca accccgtgtt
     1321 ctggctcttg tcgagcttgc ccagcagttc cttgagcgcg gccagcgccg ggctaagctc
     1381 tacggacgct tcgacaatgg tttcgttggc ggcgtacgtg cggctgtcca cggtggcctt
     1441 gcgtttgcgc ttgccggtgc catcggcgcc cggctcgtca gcggcagccg ccgcggctgc
     1501 ggcatcctcc tgcgcctgcc gggcggcttc ttcggcctcg gctttggccg ccgccagccg
     1561 cgcggccacc tcggccagct tcgggttggc atcacccacc ggcagccaaa agttcttgaa
     1621 cagcttgctc aaaccacggt agtgctgctc ggccaggatc tccgttggcg ccatcagcgc
     1681 gccctgcgcc ccgtcggcaa tcgccgcgag caaggccgta gccgcgacca ccgtcttgcc
     1741 ggagccgaca tcgccctgca ccagccgggc catcggcaga tcgcgctcca tgtcggcggt
     1801 gatctcctcc agcacgcgct gctgggcgcc ggtcaactgg aaaggcagcg tctcgcggaa
     1861 gctgttgagg atgtcggggc gcacccggaa ggccaggccc ggctggtcgt gctgccaatg
     1921 ctgtttgcgc tgcaacatgc ccaactggat caggaagaac tcgtcgaagg ccaggcggcg
     1981 gcgcccttga ttcagcgtac cccagtcggc ggggaaatgg acctggcgca aggcgtcggg
     2041 caagcctaac agacc
//
LOCUS       DASUYC010000030         3616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_103522,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000030 DASUYC010000000
VERSION     DASUYC010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103522"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="VF276_00445"
     CDS             <1..727
                     /locus_tag="VF276_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605359.1"
                     /translation="FDLRRGGPAQPLITVPTERGPAPGRYGLQSAFVPAGPRAGQILL
                     TWAEPGGDRQILRLLDPAQPAVPLLTAYTGHRYSKLRLVGVDPAGDGLILANNPNIRP
                     PDGAGGVLVYLDGTGRVRETRVPLAPGAGFLGAWVRAGRLIYSTAGTDGQNPRLNLAV
                     AGLPLDQLGAAGVPPTWIYRDVVAQEGRAATLPFHPGPGLLAYVTEDGQIHTRTYTTG
                     ADVPLAATAAGFSPVSARPETTP"
     gene            925..2607
                     /locus_tag="VF276_00450"
     CDS             925..2607
                     /locus_tag="VF276_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX6605360.1"
                     /translation="MSTSNAMGGWIEINRRVPMRDGVELSADIYYPARPDGGGPWPAI
                     VLRTPYVKANPTLVKTGRYWAEHGYVFVAMDVRGRGDSDGVFVPYFNEGRDGYDSIEW
                     CAAQPWCDGNVGTLGGSYLGRVQWLAALEAPPHLRAMVVLVPPSDPFVETPTGLPSPM
                     HLCWLHGVSGRVNQPMEAVDWMRVYEHLPVRTMDELTGQPQPRWREEVDHAQLDDFWA
                     QICYQDKFDRLAVPVLHISGWYDDEQIGTPLNFIGMSNHAATPEARRSQRLLMGPWDH
                     AVNTASKLGEVDFGSQAIIDLLGEERRWFDRWLKGSPIAVPDAPARIFIMGTNAWRDE
                     QEWPLARTQWTPYYLHSAGHANSRFGDGLLSPTPPAAEPPDAYRYDPARPVPFLTAPT
                     SQQIGGPDDYAAVEQRADVLVYMTPPLPADTEVTGPIRVDLYAASSAPDTDFTAKLVD
                     VWPTGFVQRLTDGIVRARFRDGMAAPSLITPGAVYRYTIDCWNTAQVFRAGHRIGLEI
                     SSSAFPKYDRNPNTGASLGQTADLAPADQRIYHDADHPSALILPIIPRPAQQ"
     gene            complement(2675..3553)
                     /locus_tag="VF276_00455"
     CDS             complement(2675..3553)
                     /locus_tag="VF276_00455"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6605361.1"
                     /translation="MILVVGATGQLGGMITRRLLALGLPVRILVRPTSAYTPLVAAGA
                     HPVFGDLKDQHSLNVACEGVEQVLTTANSALRGGADTVQTVDLDGNRRLIDAAAKAGV
                     QQFVFTSALGADVHSPDAFMQGKARSEAHLRASGLPHTILAPNIYMEVWAAAVVGRPA
                     LAGQPVTLVGEGRRKHTMIAMGDVMEYATRLLSHYTGTHPYLPLGGPEALSWRDIVAT
                     FERILGRHIPVQYVRPGDPVPGLPPTMVGLLAAMEQFDSPVDMHDLAQAFHITPTPLE
                     AVVRRDYAPALAGTLA"
BASE COUNT          541 a         1327 c         1231 g          517 t
ORIGIN      
        1 gttcgacctg cggcgcggcg gcccggccca gcccttgatc acggtgccca ccgagcgtgg
       61 cccggcgccg ggtcgctacg gactccagag tgccttcgtc cccgccgggc cgcgcgccgg
      121 gcagatcctg ctgacctggg ccgagccggg cggcgaccgc cagatcctgc gcctgctcga
      181 tccggcccag cccgcggtgc cgctgctgac cgcctacacc gggcaccgct acagcaaatt
      241 gcggctggtg ggcgtcgatc cggcgggcga cgggctgatc ctggcgaata atcctaatat
      301 ccgcccgccg gacggcgcgg gcggcgtgct ggtctatctc gacggcacgg gccgggtccg
      361 cgagacgcgc gtgccgctgg cgccaggcgc cgggttcctg ggcgcctggg tgcgcgccgg
      421 gcgcctgatc tacagcacgg cgggcacgga tgggcaaaac ccgcggctga atctggccgt
      481 ggccgggctg ccgctggatc aactcggggc ggcgggggtg ccgcccacct ggatttaccg
      541 cgacgtcgtg gcgcaggagg gccgggctgc tacgctgccc ttccaccccg gccccggcct
      601 gctggcctac gtcaccgagg acggccagat ccacacgcgc acctacacca ccggtgccga
      661 cgtgccgctt gccgcgactg ccgccggctt ctccccggtg tccgcccgcc cggaaaccac
      721 cccttgatcc ccagcgccat ttcttcaccg cgaagacgcg aagaacgcga agaataaaac
      781 ctttataaag aatcttcgcg tccttcgcgc cttcgcggtg aatttttaaa cggcggcggt
      841 gacgtttggc gcgatttctg gtaccatggg ggcggcccct cccgccggga gggtagcgcc
      901 tgaccccaga aaggaatcgc gagcatgtct acgagcaacg ccatgggcgg ctggatcgag
      961 atcaaccggc gggtgccgat gcgcgacggc gtcgaactgt ccgccgacat ctactacccg
     1021 gcccgccccg acggcggcgg gccctggccg gccatcgtgc tgcgcacgcc ttatgtgaaa
     1081 gccaacccca ccctggtcaa gaccgggcgc tactgggccg agcacggcta cgtcttcgtc
     1141 gccatggacg tgcgcgggcg cggcgactcc gacggcgtgt ttgtgcccta tttcaacgag
     1201 ggccgcgacg gctacgacag catcgagtgg tgcgcggcgc agccatggtg cgacggcaac
     1261 gtgggcacgc tgggcggctc gtacctgggg cgggtgcagt ggctggccgc gctcgaagcg
     1321 ccgccccacc tgcgggccat ggtcgtgctg gtgccgccgt ccgacccctt tgtcgagacc
     1381 cccaccggcc tgcccagccc catgcacctc tgctggctgc acggcgtcag cgggcgcgtg
     1441 aaccagccga tggaggccgt ggactggatg cgcgtctacg agcacctgcc ggtgcggacg
     1501 atggacgagc tgaccggtca gccgcagccg cgctggcgcg aggaagtgga ccacgcccaa
     1561 ctcgacgact tctgggcgca gatctgctac caggacaagt tcgaccggct ggccgtgccg
     1621 gtgctgcaca tctccggctg gtacgacgac gagcagatcg gcacgccgct caatttcatc
     1681 ggcatgagca accacgccgc cacgcccgag gcgcggcgca gccagcgcct gctgatgggg
     1741 ccgtgggacc acgccgtcaa cacggcgtcg aagctgggcg aggtggactt cgggtcgcag
     1801 gcgatcatcg acctgctggg cgaggaacgg cgctggttcg accgctggct caagggcagc
     1861 cccatcgccg tgcccgacgc gcccgcgcgc atcttcatca tgggcacgaa cgcatggcgc
     1921 gacgaacagg agtggcccct ggcccgcacg cagtggacgc cctactacct gcacagcgcc
     1981 gggcacgcca acagccgctt cggcgacggc ctactctcgc ccacgccgcc cgccgccgag
     2041 ccgcccgacg cctaccgcta cgacccggcc cggcccgtgc ccttcctgac cgcgcccacc
     2101 tcgcagcaga tcggcggccc cgacgactac gccgccgtcg agcagcgggc cgacgtgctg
     2161 gtctacatga ccccgccgct gcccgccgac accgaagtga cgggcccgat ccgcgtggac
     2221 ctttacgccg cgtcgtccgc gcccgacacc gatttcacgg ccaagctggt cgatgtctgg
     2281 cccaccggct tcgtgcagcg cctgaccgac ggcatcgtgc gcgcccgctt ccgcgacggc
     2341 atggccgcgc cttcgcttat cacgcccggc gcggtgtacc gctacaccat cgactgctgg
     2401 aacacggcgc aggtgttccg ggcggggcac cgcatcggcc tggagatcag ctccagcgcc
     2461 ttccccaagt acgaccgcaa ccccaacacc ggcgcctcgc tcggccagac cgccgacctg
     2521 gcccccgccg atcagcgcat ctaccacgac gccgatcatc cctcggcgct gatcctgccg
     2581 atcatcccac gcccggccca gcagtgagcg ggatgcgcgc gggcgcgacc cctacagggg
     2641 tcgcgcccgc atacgccgga ggcgcggtgg ccggttaagc gagggtgccc gccagggccg
     2701 gggcgtagtc gcgccgcact acggcctcca gcggcgtcgg agtgatgtgg aaggcctgcg
     2761 ccaggtcgtg catgtccacg ggcgagtcga actgctccat ggcggccagc aggcccacca
     2821 tcgtcggggg caggccgggc accgggtcgc cggggcgaac atattgcacg gggatgtgac
     2881 ggcccaggat gcgctcgaag gtggcgacga tgtcacgcca cgacagcgct tccggcccgc
     2941 ccagcggcag gtagggatgg gtgccggtgt agtggctcag caggcgcgtg gcgtattcca
     3001 tcacgtcgcc catggcgatc atggtgtgtt tgcgccggcc ttcgcccacc agggtgacgg
     3061 gttggcccgc cagcgccggg cggcccacca cggccgcggc ccagacttcc atatagatat
     3121 tcggggccag gatcgtgtgc ggcagcccgc tggcccgcag atgggcttcg ctccgggcct
     3181 tgccctgcat gaaggcatcg gggctgtgga catcggcgcc cagggccgac gtgaagacga
     3241 actgctgcac cccggccttg gcggcggcgt cgatgaggcg gcggttgccg tcgaggtcta
     3301 cggtctgcac cgtatcggcc ccgccgcgca atgccgagtt cgcggtggtc agcacctgct
     3361 ccacgccctc gcaggccacg ttcaacgaat gttgatcctt caaatcgccg aagaccgggt
     3421 gcgcgcccgc ggcgaccagc ggggtatacg ccgaggtagg gcgcaccagg atgcgcaccg
     3481 gcagccccag ggccagcagc cggcgggtga tcatgccgcc cagttgtcca gtcgctccca
     3541 ctaccaggat catagcactg ctcctttccg ggcccgacgg acgggaatca ggccgtcgtg
     3601 taggcgactg cccgcc
//
LOCUS       DASUYC010000031         2742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1712160, whole genome shotgun sequence.
ACCESSION   DASUYC010000031 DASUYC010000000
VERSION     DASUYC010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1712160"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2222
                     /locus_tag="VF276_00460"
     CDS             <1..2222
                     /locus_tag="VF276_00460"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX6605362.1"
                     /translation="FTSAITMPLTVRDRTLGALTLVSAESGRHYGPADVALAEDLARR
                     AATAIDNARLYQEVAEQRERFAVTLGSIGDAVIATDARGQITFLNHVAADLTGWDPAA
                     AAGQDLATVFHIVNEETRALVENPATRVIREGVTVGLANHTILIDRQGGEHPIDDSGA
                     PIRDAGGALLGVVLVFRDISGRKADEAALRESEERFRTLVDSAPVLVWLAGPDGGRTF
                     FNRPWLDFTGRTAAQEQGAGWWEGVHPDDVARCREIYDTALAARDPFRMEYRLRHADG
                     VYRWVLDTGVPRWAPGGAFAGFIGSCIDITERQESEERLRFLTEAGALLSASLDYEAT
                     LASVAHLTVPAIADNCAIDLVYEDTGLRRVVIDAADPQRGELLRDLYRRYPPDPRALG
                     IFEVLESGRSRLVSQVDDAQRVAPARDAAHAELLRAAGTKSYMVVPFVARGRVIGTLA
                     LNMAESGRHYGPADLALAEALAARAALAVDNARLYQDAQAAIGTRDQFLSIASHELKT
                     PLTSLLMAAQLIQRRTAREGTLNPRDQRALTVVVNQASRLNRMVLSLLDLSRIESGQL
                     SIEEGEVDLTALVRNLQEEVTPALEQHTLVVEAPAAPVLVRGDALRLEQVLQNLVQNA
                     VRYSPEGGPITVLVATQDGEARVSVTDRGIGIPAHALPNLGGRFYRAPNVERHQISGL
                     GIGLYVVNEIVALHGGRLDVTSTEGEGSTFTIYLPLLTAPGALPAGDAAAIPDRDPV"
     gene            complement(2306..2434)
                     /locus_tag="VF276_00465"
     CDS             complement(2306..2434)
                     /locus_tag="VF276_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605363.1"
                     /translation="MFTNAYQLQHLARETTQARTREARQRQIARRFANWRRIQAAR"
BASE COUNT          386 a          974 c          980 g          402 t
ORIGIN      
        1 gcttcacctc ggccatcacc atgccgctga cggtgcgcga ccgcacgctg ggcgcgctga
       61 cgctggtctc cgccgagtcg gggcggcact atggcccggc ggatgtggcc ctggccgagg
      121 acctggcccg ccgcgccgcc acggccatcg acaacgcccg gctctaccag gaggtggccg
      181 agcagcgcga gcgcttcgcc gtgacgctcg gtagtatcgg cgacgcggtg atcgccaccg
      241 acgcgcgcgg ccagatcacc ttcctgaacc acgtggcggc ggacctgacg ggctgggacc
      301 ccgccgccgc ggcgggccag gacctggcga ccgtgttcca tatcgtgaac gaggagacgc
      361 gggcgctggt cgagaacccg gccacgcgcg tgatccgcga gggcgtgacg gtgggcctgg
      421 cgaatcacac catcctcatc gaccgccagg gcggggagca ccccatcgac gacagcgggg
      481 cgcccatccg ggatgccggc ggcgcgctgc tcggcgtggt gctcgtcttc cgcgacatca
      541 gcgggcgcaa ggccgacgag gcggcgttgc gcgagagcga agagcgcttc cgcaccctgg
      601 tagacagcgc gccggtgctc gtctggctcg ccgggcccga cggcgggcgc actttcttca
      661 accggccctg gctcgatttc accgggcgta ccgcggccca ggagcaggga gcgggctggt
      721 gggaaggcgt gcatcccgac gacgtggccc gctgccgcga gatctacgac accgccctgg
      781 ccgcccgcga ccccttccgc atggagtacc gcttgcgcca cgccgacggc gtctatcgct
      841 gggtgctcga caccggcgtg ccccgctggg cgcccggcgg ggcgtttgcc ggttttatcg
      901 gctcctgtat cgacatcacc gagcgccagg agagcgagga gcggctgcgc ttcctgaccg
      961 aggcaggcgc gctgctcagc gcctcgctcg actacgaggc caccctcgcc agcgtggccc
     1021 acctgaccgt cccggccatc gccgacaatt gcgccatcga cctggtctac gaagataccg
     1081 ggctgcgccg ggtggtcatc gacgccgccg atccgcaacg gggcgagttg ctgcgcgacc
     1141 tttaccgccg ctacccgccc gacccgcgcg cgctgggcat cttcgaggtg ctggagtcgg
     1201 gccggtcgcg gctggtatcg caggtggacg acgcgcagcg ggtggccccg gcccgcgacg
     1261 ccgcgcatgc ggaactgctg cgcgccgcgg ggaccaagtc ctacatggtg gtgccgttcg
     1321 tggcccgcgg gcgggtgatc ggcacgctgg ccctgaacat ggccgaatcg ggccgccact
     1381 acgggccggc ggacctggct ctggccgagg ccctggccgc ccgcgccgcg ctggccgtgg
     1441 acaatgcgcg cctgtaccag gatgcgcagg ccgccatcgg cacccgcgac cagttcctgt
     1501 cgatcgcctc gcatgaactc aaaacgccgc tgacctcgct gctgatggcc gcgcagctga
     1561 tccagcgccg caccgcgcgc gagggcacgc tcaacccgcg cgaccagcgc gccctcaccg
     1621 tcgtggtcaa ccaggcgtcg cggctcaacc gcatggtgct gtccctgctc gacctgtcgc
     1681 gcatcgaaag cgggcaactg agcatcgagg aaggcgaggt ggacctgacg gcgctggtgc
     1741 gtaacctgca ggaggaggtg acgccggcgc tggagcagca tacgctggtg gtagaggcgc
     1801 ccgcggcccc ggtgctggtg cgcggcgatg cgctgcgcct ggagcaggtg ctgcaaaacc
     1861 tggtgcagaa cgccgtgcgc tacagccccg agggcgggcc gatcacggtc ctcgtggcga
     1921 cgcaggacgg cgaggcccgc gtctcggtca cggatcgcgg catcggcatc ccggcccacg
     1981 cgctgcccaa cctgggcggg cgcttctatc gcgcgcccaa tgtcgagcgg caccagatca
     2041 gtggcctggg catcggcctc tacgtggtca acgagatcgt ggcgctgcac ggtggccgcc
     2101 tcgacgtgac cagcacggag ggcgagggca gcacctttac catctacttg ccgctgctca
     2161 ccgcgccggg cgcgctgccg gcgggcgacg ccgccgccat ccccgaccgc gacccggtgt
     2221 gaggctgcgg tcaggaggaa agcccggcag gcccgcggaa ctccgcgggc ctgccattta
     2281 tatgagggag gttgggggcc gggcgttagc gcgcggcctg gatgcggcgc cagttggcga
     2341 accggcgggc gatctggcgc tgccgggctt cgcgcgtgcg ggcctgagtc gtctcccgtg
     2401 cgaggtgctg caactggtat gcgttggtga acatcgtcgt ttcctctctc cgggtgggtc
     2461 ggctccggcg ccgggtcatt gtcgcgcacg tttgcttgat gggtccatct taccaccggc
     2521 gcgcgtggcg cacatcggga gggggctgta gccgtgccca tgcattggcc ccagcgcgcc
     2581 cccggcaggc ggacaggcgg cggctacatc tccagaccga ggccgctcca tcgagcgacc
     2641 ccagccgtaa tccgcttgat ctacagtcat aattgtcatg ctgagcgcag cgaagcatct
     2701 cgtcttggtc ccgcacgaga cgagactctt cgctgcgctc ag
//
LOCUS       DASUYC010000032         3434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_556037,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000032 DASUYC010000000
VERSION     DASUYC010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556037"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VF276_00470"
     CDS             <1..489
                     /locus_tag="VF276_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605364.1"
                     /translation="SAGGGGGAGSGNGYLGRRAGWSPAAREADELEELAAKSCILPQN
                     NTLQNKDQPFIDELAIQLRRIFGSENVRTNFPVINSKGKPVEIDIETPNAIIEVKSGG
                     ATGLGSQVEVRVKDPMLNPTGKPVIGYGPERMKGAVYDYINTHGGIAARNIDEILDII
                     VP"
     gene            524..937
                     /locus_tag="VF276_00475"
     CDS             524..937
                     /locus_tag="VF276_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605365.1"
                     /translation="MEDLFYFPPEPISVDDIADVARDAGLTFQTHPPDDLTIYYTDDN
                     FWSWQELHDDGGADMVGFEEADRATMAALSYAKVFMISLRPASLPKLCEFLAMVLQRY
                     GGWIGSDSEGLYPIFTSANIADLAHMRLGYGPLSG"
     gene            complement(989..3100)
                     /locus_tag="VF276_00480"
     CDS             complement(989..3100)
                     /locus_tag="VF276_00480"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX6605366.1"
                     /translation="MNWEDVPALLDRLAEVWPGYSPRPGQRQMARAVAHAMDSGLNLL
                     VEGGTGVGKSVAYLLPAALSALEDDKRTLIATSHKHLQDQLSEKDLPRVDALVRDLGF
                     PGLAWTTIKGLPNYVCRVAADSELALADPHPLIDRVADWMEAPGHHGEFDELPFAIPA
                     DLRSRLSTEAEECPHERCPRYHDCFAMAALRRAKAAPIVITNHALLGLHVAAGGHLLP
                     GPFDNVVVDEAHAFEDAATRAYGFECSSFALRRLLHSDIVRRTAERPLLREAEAALAR
                     LDLEVAALLGVAVAGLPLQQAALDDAEPAAADYTGSHVDIEAVPGPALDAGGINRTLL
                     HAPLAAGHALADLLDKLATDLDPDRRILVDQYPYDRSPFDLDQPALDELSETETAMAR
                     AGKRAASLADRLRTVCGADDPLLVYYVEQVGSAGAGLPAYNLLALPLEVGLFLEAWWE
                     EQATILTSATLSDGQSFSFFSDRLGLARPRKLVVPSPFDYPTRTRLVLTPVPGQDKAG
                     DAYFARLADQIARLMDAAGGKCLLLFTSHRALDAVWQRLSIMRIAGWRLYRQGETSQA
                     QLLTALRQAAPDDRIAMFASRSWWQGVDLPGMRLVVLDKLPFPQLGDPLVAARTAQIE
                     DLEGQSFRRYMLPLALIAFRQGFGRLMRSETDFGAVAVCDARLGTRQYGARFLAALPE
                     GIPVLGSSDELRAWVRGMAAG"
BASE COUNT          595 a         1065 c         1188 g          586 t
ORIGIN      
        1 tccgcaggcg gcggtggagg tgcgggtagc ggcaacggct acctgggacg gcgcgctggg
       61 tggtcgccgg cggcccgcga agcggacgag ctagaagagc tagcagcgaa gagttgtatt
      121 cttccccaga ataatacgct ccagaataag gatcaaccct tcatcgatga gctcgctatt
      181 caattacgcc ggatctttgg tagtgaaaac gtgcgcacta attttccagt gatcaatagc
      241 aaaggtaaac ctgtagagat cgatattgaa acgccaaatg ccattatcga ggttaaaagc
      301 ggtggtgcaa ctggtctggg tagccaagtc gaagtacgag tcaaagatcc gatgcttaac
      361 ccaactggaa agcctgttat cggatacgga cctgagagga tgaaaggggc ggtgtacgat
      421 tacattaata cgcatggtgg cattgcagcg cgtaacattg acgagatact tgatataatt
      481 gtaccttgag aacggaccat gaatcgagca caaggagata gctatggaag atttgttcta
      541 tttcccacca gagccgatat cggtagatga catagcggat gtcgcgcggg atgcgggact
      601 tactttccag acccatcctc cagatgatct taccatctac tacaccgatg ataacttctg
      661 gtcctggcaa gaactacatg atgacggtgg ggccgacatg gtagggttcg aggaggcgga
      721 tcgcgcgacg atggctgcgc tgagctacgc caaagtgttt atgatttcgc ttcgcccggc
      781 gtccttgccc aagctatgcg agttcctggc tatggtttta cagcgctacg gcggctggat
      841 cgggtctgat agcgaggggc tgtatccgat atttacctcg gccaacatcg cagaccttgc
      901 tcacatgcgc ttaggctacg gaccactatc gggttagcat ttaggttcta ctcctcggcc
      961 aaataccagg atgacggggc tatgccgctc accccgccgc catgccgcgc acccaggcgc
     1021 gcagttcgtc gctgctgccc aggacgggga tgccttcggg gagcgccgcc aggaagcggg
     1081 cgccgtactg gcgcgtgccc aggcgggcgt cgcagacggc caccgcgccg aagtcggtct
     1141 cgctgcgcat gagccggcca aagccctggc ggaaggcgat cagggccagc ggcagcatgt
     1201 agcggcggaa cgactggcct tccaggtcct cgatctgggc ggtgcgcgcg gccaccagcg
     1261 ggtcgcccag ttgcgggaag ggcagcttgt ccagcaccac caggcgcata ccgggcaggt
     1321 ccacgccctg ccaccaactg cggctggcga acatggcgat ccggtcgtcg ggggcggcct
     1381 ggcgcagggc ggtgagcagt tgggcctggc tcgtctcgcc ctggcggtag agccgccacc
     1441 cggcgatacg catgatgctc agccgctgcc agacggcgtc gagcgcgcgg tggctggtga
     1501 acagcagcag gcacttgccg cccgcggcgt ccatcaggcg ggcgatctgg tcggccaggc
     1561 gggcgaagta ggcgtcgcca gccttgtcct ggccgggcac gggggtcagc accaggcggg
     1621 tgcgggtggg gtagtcgaag ggcgagggca cgaccagttt gcgcgggcgg gccaggccca
     1681 gccggtcgct gaagaagctg aacgactggc cgtcggacag ggtggcgctg gtcaggatgg
     1741 tcgcctgctc ctcccaccag gcttcgagga acagccccac ctccagcggc agcgccagca
     1801 ggttgtacgc gggcagcccg gcccccgccg accccacctg ctcgacatag tagaccagca
     1861 gcgggtcgtc ggcgccgcag acggtgcgca gccggtcggc cagcgaggcg gcgcgcttgc
     1921 cggcgcgggc catggccgtc tcggtctcgc tcagttcgtc gagcgcgggc tggtcgaggt
     1981 cgaagggcga gcggtcgtag ggatactgat cgaccaagat gcggcggtcg gggtccaggt
     2041 cggtcgccag cttatcgagc agatcggcca gggcgtgccc ggcggccagc ggcgcgtgca
     2101 acagcgtgcg gttaatgccc ccggcgtcga gcgcgggacc gggcacggct tcgatatcga
     2161 catggctgcc cgtgtagtcg gcggctgccg gttcggcgtc gtcgagcgcg gcctgctgga
     2221 gcggcagccc ggccacggcc acgccgagca gcgccgccac ttcgagatcg agccgggcca
     2281 gcgccgcctc ggcctcgcgc aagagcgggc gttcggcggt gcggcgcacg atgtcgctgt
     2341 gcagcagccg gcgcagggca aacgacgagc actcgaagcc gtaggcccgc gtggccgcgt
     2401 cctcgaaggc gtgcgcctcg tccaccacga cgttgtcgaa cggccccggc agcaggtggc
     2461 cgcccgccgc gacatgcagg cccagcagcg cgtggttggt gatgacgatg ggggcggcct
     2521 tggcccggcg cagcgcggcc atggcgaagc aatcgtggta gcgcgggcag cgctcgtgcg
     2581 ggcactcctc ggcctcggtg ctcaggcgcg agcgcaggtc cgccgggatg gcgaagggca
     2641 gttcgtcgaa ctcgccgtgg tggccgggcg cctccatcca atcggccacc cggtcgatca
     2701 gcgggtgcgg atcggccagc gccagttcgc tgtcggcggc cacccggcag acatagttgg
     2761 gcaggccctt gatcgtggtc caggccaggc cggggaagcc caggtcgcgc accagggcgt
     2821 cgacgcgcgg caggtccttc tcgctcaact ggtcttgcag gtgcttgtgc gacgtggcga
     2881 tgagggtgcg cttgtcgtct tcgagcgcgc tgagcgcggc gggcagcagg taggccaccg
     2941 acttgccgac gccggtgccc ccctcgacca gcagattcag ccccgagtcc atcgcgtggg
     3001 ccacggcgcg cgccatctgg cgctgaccgg gccgcgggct gtagcccggc cacacctccg
     3061 ccagccggtc cagcagcgcg ggcacatctt cccagttcat ttgttctata cccccacccc
     3121 cattataccg aattcgcttc tcgccttgca cgtgtttatg ctcaccgcag aggcgcagag
     3181 gacgcagagg atttttgggg tttattcggt cgccccacca cgctgtcatt ctgagcgcag
     3241 cgaagaatct ccgtctcgtt cggggtcacg ccgagactct tcgctgcgct cagagtgaca
     3301 agcctatagc cgtatctctg cgtcctcggc gcctctgcgg tgaagctcgt aaggcaggtg
     3361 gagactcctt ccggagattg aggggtgatc cgcctcgcgg tgcatcccgc cgtggcgccg
     3421 atggggtaga ctgg
//
LOCUS       DASUYC010000033         2918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_54512,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000033 DASUYC010000000
VERSION     DASUYC010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54512"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            286..1701
                     /locus_tag="VF276_00485"
     CDS             286..1701
                     /locus_tag="VF276_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007109757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX6605367.1"
                     /translation="MLEEAIRPAVVLDDVEVRELEDELAGALIRPTDAEYDSARQTWN
                     ARFNRRPALIVRPAGDEDVVRAVRFAKRNDLTLAIRGGAHSLAGLGVADDGLVLDMSG
                     MRRITVDPAARVARVQAGARWKDLEAVAGPLGLATPSGTCPTTGVAGVALGGGVGWLT
                     RHYGLSIDNLLGVDIVTADGVLRHASATENPDLFWAVRGGGGNFGVVTMLEMRLYPVT
                     MLYGGMIGWPMAQAGAVLRHFREFAAAAPDEFGSIVMFMTAPPAPFVPPALHGQPVIA
                     IAVAYSGDLAEGARVVAPLRAFGAPAVDTLGPLPYAAMQAIGEPAGEMGVQHYIKSGY
                     LDTLSDAAIDTIVAYAAALPGPMTALHLNQIGGAMTRVPADATAFGRRTAQFIVNAMP
                     TWVDPAQGAAHRAWADTFLAAMAPVLGDGVYVNFLDAESPERVRAAYGANWDRLRAVK
                     RAYDPTNFFHQNQNIPPADEG"
     gene            2004..2324
                     /locus_tag="VF276_00490"
     CDS             2004..2324
                     /locus_tag="VF276_00490"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6605368.1"
                     /translation="MILARNDAGRPAKGWYTELFQPALPAIGFANAGINEPHYHQELR
                     EVYLVARGSSTMIVDEQKITLQAGDVIVVEPGEVHTFVDNTPDYFHFVLHCPMIRGDK
                     VAVP"
     gene            2354..>2918
                     /locus_tag="VF276_00495"
     CDS             2354..>2918
                     /locus_tag="VF276_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX6605369.1"
                     /translation="MTTNIQNPGGGSGAASYTSLFDANFLKKLERLSVISRKLRAGRM
                     KGERRSTHRGNSVEFADYRDYTPGDDLRRVDWNAYARLERLFLKLYQEEEDITIHVLL
                     DGSKSMDWGDNKPVFGLGTVAAPGGGTPASADQNKLIYGKRTAAALGYIGLSGFDRVT
                     VAAFHRGNISRFAPVRGRGHAVNLLRFI"
BASE COUNT          474 a          977 c         1017 g          450 t
ORIGIN      
        1 tccccccttc ccgcggcggg aaggggggca ggggggttag gttgctcgcc cgcccggatg
       61 ccccctaatg cgtgcggggg cgccaggtta cgcgaaatca gcgacaggat aggcccaatt
      121 gcggcgggac aagtggccca cgcagattcg gcccgccgcc gcatccggca agcccagccg
      181 ggctgctata ctggcggcgt tgaggcaatc gcgcctcacc tgagacaata atcacccttg
      241 tatagcagcg ggcggtcccc ggatcgccca taacaggaga cgttgatgct cgaagaagcg
      301 attcgccccg cggtggtcct ggatgacgtt gaggtgcggg aactggaaga cgaactggcg
      361 ggggcgctga tccgcccgac cgacgccgaa tatgatagcg cgcgccagac ctggaacgcg
      421 cgctttaacc gccgccccgc gctgattgtg cgcccggcgg gcgacgaaga tgtggtgcgc
      481 gcggtgcgct ttgccaagcg gaatgacctg accctggcga tccgcggcgg cgcgcatagc
      541 ctggccggcc tcggcgtggc cgacgacggt ttggtgctcg acatgtcggg catgcgccgg
      601 atcacggtgg acccggcggc gcgggtggcc cgcgtgcagg cgggcgcgcg ctggaaggat
      661 ctggaggcgg tggccgggcc gctcgggctg gcgacgccct cgggcacgtg cccgacgacg
      721 ggcgtggccg gggtggcgct cggcggtggt gtcggctggc tgacccgcca ctacggcctc
      781 agcattgaca acctgctcgg cgtggacatc gtgaccgccg acggcgtgct gcgccacgcc
      841 agcgccaccg agaaccccga cctgttctgg gccgtgcgcg gcggcggcgg caacttcggc
      901 gtcgtgacca tgctggagat gcggctctat cccgtgacca tgctctacgg cggcatgatc
      961 ggctggccca tggcccaggc cggcgccgtg ctgcgccact tccgcgagtt cgcggcggcg
     1021 gcgcccgacg agttcggcag catcgtcatg ttcatgacgg cgcccccggc gcccttcgtg
     1081 ccgcccgcgc tgcacggcca accggtgatc gccatcgccg tcgcctattc cggcgacctg
     1141 gcggaaggcg cccgcgtggt cgccccgctg cgcgcgttcg gcgcgcccgc cgtggacacc
     1201 ctcggcccgc tgccctacgc ggcgatgcag gccatcggcg agcccgccgg ggagatgggc
     1261 gtccagcact atatcaagtc gggctacctg gacacgctct cggacgccgc catcgacacg
     1321 attgtggcct acgccgctgc cctgcccggc ccgatgacgg ccctgcacct caaccagatc
     1381 ggcggcgcga tgacccgcgt gcccgcggac gcgaccgcgt ttggccggcg caccgcccag
     1441 ttcatcgtca acgccatgcc cacctgggtc gatccggcgc agggcgccgc ccatcgcgcc
     1501 tgggccgaca ctttcctcgc cgccatggcc ccggtgctgg gcgacggggt ttatgtcaac
     1561 ttcctggacg ccgaaagccc ggagcgcgtg cgcgcggcct acggcgccaa ctgggatcgc
     1621 ctgcgcgccg tcaagcgcgc ttacgacccg accaacttct tccaccagaa ccagaacatc
     1681 ccgcccgcgg acgagggctg agcggacgag tggtcagtgg tcagcgattg gccgtcagct
     1741 gtcagccgtc aggagacgag tggtcagtgg tcagtggtca gtggtcagtg acgaacgaag
     1801 cttgtcacaa ggccatcagg gcttctgata aaagccggga tgctggtgta ggggcgggtt
     1861 tcaaacccgc cgtgccgccc tacgcgccct gtcgattgcc agcgtagtgc ggcgaagagt
     1921 ctccggcgcc ccaaacgaga cgagattctt cgctgcgctc agaatgacca gaggaagcca
     1981 caggcgctac cgaggaggcc cgcatgatcc tggcgcgcaa cgacgcgggg cggcccgcga
     2041 agggctggta tacggagttg tttcagccgg cgctgcccgc tatcggcttt gccaacgccg
     2101 gcatcaacga gccgcattac caccaggagt tgcgggaggt ctatctggtg gcgcggggca
     2161 gcagtaccat gatcgtggac gagcagaaga tcacgttgca ggcgggcgat gtgatcgtgg
     2221 tcgagccggg cgaggtgcac acatttgtcg acaacacgcc cgactacttc catttcgtgc
     2281 tacattgccc gatgatccgg ggcgataagg tggcggttcc ttagcactat ttgcttagaa
     2341 cggtagggat acgatgacaa ccaacatcca gaatcccggc gggggcagcg gggccgcctc
     2401 ctatacctcc ctcttcgacg ccaacttcct caagaagctg gagcggttga gcgtgatcag
     2461 ccgcaagctg cgcgccgggc ggatgaaggg cgagcggcgg tccacgcatc gcggcaactc
     2521 ggtcgagttc gccgattacc gggactacac gcccggcgac gacctgcggc gcgtggactg
     2581 gaacgcctat gcccggctgg agcgcctgtt cctcaagctc taccaggagg aagaggacat
     2641 caccatccat gtgctgctcg acggcagcaa gtcgatggac tggggcgaca acaagccggt
     2701 gttcgggcta ggcacggtgg cggcgccggg cggcggcacg ccggcctccg ccgaccagaa
     2761 caagctgatc tacggcaagc ggacggcagc ggcgctcggc tatatcggcc tgagcggctt
     2821 cgaccgggtg acggtggcgg cgttccaccg gggcaacatc agccgcttcg cgccggtccg
     2881 cgggcgaggc cacgcggtca acctgctgcg ctttatcg
//
LOCUS       DASUYC010000034         2948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1490378, whole genome shotgun sequence.
ACCESSION   DASUYC010000034 DASUYC010000000
VERSION     DASUYC010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1490378"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VF276_00500"
     CDS             <1..543
                     /locus_tag="VF276_00500"
                     /inference="COORDINATES: protein motif:HMM:NF016051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEX6605370.1"
                     /translation="ALDLGAAVTLIHGPVQLAPLYGVTAVRVGTTRDLEAAVRAAVPE
                     AAALIMAAAPADYRPAAVSEQKIKKQPGQDTFTLDMVRNPDILAGLRDLPGIEGLVRV
                     GFAAETEQLLANAQSKLAAKGLDLIVANEATRSIGAPDSAVLLIDQAGVEELGIQPKS
                     TTARHILARVVALLRRNGSL"
     gene            623..1066
                     /locus_tag="VF276_00505"
     CDS             623..1066
                     /locus_tag="VF276_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605371.1"
                     /translation="MRTEDVLAACLDAQAQGRSPAAILDHDATPEQRAELESLLDLAG
                     RLGRLAPPGLPATRREAMGRRLAAALGAPAGQPDPYDLGRTPEVHSLAPVLELSREDL
                     WRYIGPDGEAKIGRFLDVPGYTTMFLALRLFFRGVKRVENLGLTF"
     gene            1104..1406
                     /locus_tag="VF276_00510"
     CDS             1104..1406
                     /locus_tag="VF276_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX6605372.1"
                     /translation="MTFTVNFSEAGHWLVLLLIAALAGLIVELIRGGAIPLGFVGEMV
                     FAFLGAWLGGDVLQTRFPVLPQPALENVALIPAAVGALVVGLLWGLLGGRRRRGYY"
     gene            1564..2067
                     /locus_tag="VF276_00515"
     CDS             1564..2067
                     /locus_tag="VF276_00515"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX6605373.1"
                     /translation="MPDKTHENPRADTDQVDDEAVQDEDVVEPDAGGAISSIGLGRRR
                     ASRPLPPLSRSTAGSPINADRPVFTLSVAADLLGMHPRTLRIYEEEGLVVPARTPTKR
                     RRYSQNDIKRFQFIQHLTQRLKVNLAGVRIILEMMTALESCGVDFMPRLYEMGEQAAS
                     DNNNTPH"
     gene            2555..>2948
                     /locus_tag="VF276_00520"
     CDS             2555..>2948
                     /locus_tag="VF276_00520"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HEX6605374.1"
                     /translation="MLPMKNLFNVVKRSVSEYSQDNVANMAAALAFAAFFSIFPLILF
                     LVSLASFLLSPEQARQWVLQNVLPNLQSSTDESNVFVKTITDVVAARGTGTGIAALIG
                     LVSLLLSASGVFGTLQLAINRAWECDKAG"
BASE COUNT          521 a         1018 c          907 g          502 t
ORIGIN      
        1 gcgctcgacc tgggcgccgc cgtcaccctg atccatggac ccgtgcagtt ggccccgctc
       61 tacggcgtca ccgccgtccg ggtgggcacc acgcgcgatc tggaagcggc ggtgcgggca
      121 gcggtgccgg aggccgccgc cctgatcatg gccgccgccc cggcggacta ccgccccgcc
      181 gccgtgtcgg agcagaagat caagaagcag cccggccagg ataccttcac gctcgacatg
      241 gtgcgcaacc ccgacatcct ggcgggcctg cgcgatctgc ccggcatcga gggcctcgtg
      301 cgcgtcggct ttgccgccga aaccgagcag ttgctggcga acgcgcagag caaactggcc
      361 gccaaggggc tggatctgat cgtcgccaat gaggccacgc gcagcatcgg cgcgcccgac
      421 agcgcggtcc tgttgatcga tcaggcaggc gtggaagaac tgggcatcca gccaaaaagc
      481 acgactgcac gccatattct ggcgcgggtg gtcgcgctgc tgcgtcgtaa cggcagcctc
      541 taaaccggcg ttataagcca tgcacgatac tagagtcgcg gatgcagcag ggacacctca
      601 ggaaaccgac agggagttac taatgcggac ggaagacgtg ctggccgcat gcctggacgc
      661 tcaggcgcaa gggcgctcac cggcggcaat actggaccac gacgccactc ccgagcagcg
      721 cgccgaactc gaatctcttc tcgatcttgc cggacgcctg gggcggctcg cgccgcccgg
      781 cttgccggcc acccgccgcg aggccatggg ccgccgcctg gccgcggcgc tgggcgcacc
      841 ggccggccag cctgacccgt atgatctggg ccgcaccccg gaggtgcata gtctcgcgcc
      901 cgtcctcgaa ttaagccgcg aggatctctg gcgctatatc ggcccggacg gggaagcaaa
      961 aatcggacgc ttcctggacg tacccggcta cacaaccatg ttcctggcct tacggctctt
     1021 cttccgcggg gtcaagcgag tcgagaacct gggactcaca ttctaatcaa ggacaagcgg
     1081 ccgctccgcc ggccccaccc atcatgacct ttaccgtgaa tttcagcgaa gccggccact
     1141 ggctggtctt gctgctcata gctgcgctgg ccgggctgat cgtcgaactg atccgcggcg
     1201 gcgccattcc cctgggcttc gtcggcgaaa tggtgtttgc cttcctgggc gcctggctgg
     1261 gcggcgacgt gttgcagacc cggttccccg tgctgcccca accggcgctg gaaaacgtgg
     1321 ccctcatccc cgccgccgtc ggcgcgctgg tcgtcggcct cctctggggc ctgctgggcg
     1381 ggcgccggcg ccgcggctac tactaaatga ccccgccgca ggcgtggggc aagatcaaaa
     1441 accggggcgc ctttaggccc aaaaacttga caacgctcat ggtctgtgtt atgatcgacg
     1501 ctgaccacag catgtctatt gccgtgctgt gcccacccaa acgccaagcg attgaggccc
     1561 agtatgccag ataaaaccca cgagaacccc cgcgccgata ccgatcaagt ggacgacgaa
     1621 gcagtacagg acgaagacgt tgtcgaaccg gatgcgggtg gggcgatctc cagtatcggc
     1681 ctgggccggc ggcgggccag ccggcccctc ccacccctgt cgcgcagcac cgccggcagc
     1741 cccatcaacg ccgaccgccc ggtgtttacg ctgagcgtgg ccgcggatct cctgggcatg
     1801 cacccgcgca cattgcggat ttacgaagaa gaagggctgg tcgtgcccgc ccgcaccccg
     1861 acgaagcgcc gccgctattc gcaaaacgac atcaagcgct ttcagttcat ccagcacctc
     1921 acccagcgcc tcaaggtcaa cctggccggt gtgcgcatca tcctggagat gatgaccgcg
     1981 ctcgaaagct gcggggtcga tttcatgccc cgcctctacg agatgggcga acaggccgcg
     2041 tccgacaaca acaacacgcc gcattaaccc ccgcgcctct gcgccggccg cccgcctgaa
     2101 acccgcctct ccccggcgta actggcgggc ttcttttttg ttcttttcac tgaataagcc
     2161 agccgacccg atttgtcact cttcgctgcg ctcagtagtc ggtcacacgg gaattgtcat
     2221 tctgagcgga gcgaagaatc tccgtcttgt agcccaaacg acgagactct gcgctgcgct
     2281 cagagtgaca atggatgctt gatgcgctgt tggcctgccc ggtcgccccg tttgccgcga
     2341 tcaccaccgc gccgggcacc ggcagcaacc gtgtcacgat aattgcttat agcgaatcta
     2401 ggggacggcc ccgcgagtac cggcagcgcg ccgggatgcg gcccggcccc gcccggcggt
     2461 gccacgccgt ccggcgagaa tccgtattaa gcattacctg gccgcggcag caccctgggg
     2521 gccgccggcg atcagttgcg tggaggtctg cgctatgctc ccgatgaaga acctgtttaa
     2581 cgtcgtcaaa cgctcagtca gcgaatacag ccaggacaat gtcgccaata tggccgccgc
     2641 cctggccttc gccgcgttct ttagtatttt cccgctcatc ctgttcctgg tgtcgctggc
     2701 gagttttctg ctctcgcccg agcaggcccg ccagtgggtg ctccagaatg tgctgcccaa
     2761 cttgcagagc agcaccgacg agtcgaacgt ctttgtcaag acgattaccg acgtggtggc
     2821 ggccaggggc accggcaccg gcatcgcggc cctgatcggt ctcgtcagcc tgctcttgag
     2881 cgcctccggc gtcttcggta cactgcaact ggcgatcaat cgcgcctggg aatgcgacaa
     2941 ggcgggca
//
LOCUS       DASUYC010000035         2216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_564135,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000035 DASUYC010000000
VERSION     DASUYC010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564135"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1747)
                     /locus_tag="VF276_00525"
     CDS             complement(<1..1747)
                     /locus_tag="VF276_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011330784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX6605375.1"
                     /translation="MPEFAHAPQPQHHPAAEPARPAPSPSPLPAAGRGRGRGWPAGHD
                     FGRMAVHAPEPGHLQAKLATNAPDDSYEQDADRLAAQVTATPGPPAASDPPPRIRRLT
                     EPPGGPGAPPSVATALAGPGTPLDPALRRDMEGRFGYDFSRVRVHTGAAAAQSARDLD
                     ALAYTTGPHLVFAAGQYAPETPPGRRLLAHELAHVAQQAAAEPLAAQAGSGPAPVAAP
                     GPVPASGVVQRAPDPAPQADPQHPFLLKYKGQPDKDRWINLEDVEWSENYIDFNMVTM
                     QVTFIPGTNLDNIDHDRAKAIHIEYENGHIVDFPLDDIPLIKDPNPRGARVGRLDYYE
                     MRDGMVYPIASGMVMYSPQNTPNLIGLRYDAYNRIEQLRQLALLRSLAGTFANLIAMS
                     VGVMGSVHEMGGMGGVPGGGGRPRRGGTQTDPPDIGREIDQAFSRLPEEDPHSGAVRD
                     PNSPIRSDVMVGGQPEAVGPSKGGVPAVKDPKGPPPELLDVGAGPQRTNLGVPPEHEL
                     VAVTRSDVSTAGNPDVVLDATKPIPANLRGRFTTLMVNNPYGYKPNIGAMREALGPDG
                     KIIVQGNWEANKYFRS"
BASE COUNT          269 a          737 c          843 g          367 t
ORIGIN      
        1 gcgaccggaa gtacttgttc gcttcccagt tgccctgcac gatgatcttg ccgtcgggcc
       61 ccagcgcctc gcgcatggcg ccgatgttcg gcttgtagcc atagggattg ttgaccatga
      121 gcgtggtgaa gcgcccgcgc aggttcgcgg ggatcggctt cgtcgcgtcc aggaccacgt
      181 cggggttgcc ggcggtgctc acgtccgagc gggtgacggc caccagttcg tgctcgggcg
      241 gcacgccgag attcgtccgc tgcgggcccg cgcccacgtc gagcagttcg ggcggcggcc
      301 ccttggggtc cttcaccgcc gggacgccgc ccttgctcgg ccccaccgct tccggctgcc
      361 cgcccaccat cacgtccgac cggatggggc tgttggggtc gcggacggcg ccgctgtgcg
      421 ggtcctcctc gggcaggcgg ctgaacgcct ggtcgatctc ccggccgatg tcgggcgggt
      481 cggtctgggt gccgccccgg cgcgggcgcc cgccgccgcc cggcacgccg cccatcccgc
      541 ccatctcgtg cacgctgccc atcacgccca cgctcatggc gatgaggttg gcgaaggtgc
      601 cggccagcga ccgcagcagc gccagttgcc gcagttgctc gatgcggttg taggcatcgt
      661 agcgcagccc gatcaggttg ggcgtgttct gcggcgaata catgaccatg cccgaggcga
      721 tggggtagac catgccgtcg cgcatctcgt agtagtcgag ccgcccgacg cgggcgccgc
      781 gcgggttggg gtccttgatc agcgggatgt cgtccagggg gaagtcgaca atgtggccgt
      841 tttcatactc gatgtggatg gctttggccc ggtcgtggtc gatgttgtcc aggttggtgc
      901 cggggatgaa cgtgacctgc atggtgacca tgttgaagtc gatgtaattc tccgaccact
      961 ccacgtcttc gaggttgatc cagcggtcct tgtcgggctg gcccttgtac ttgaggagga
     1021 aggggtgctg cgggtccgcc tggggggccg ggtccggcgc ccgttgcacc acgccggagg
     1081 ccgggaccgg gccgggcgcg gctacgggcg cggggccgga gccggcctgc gcggccaggg
     1141 gttcagcggc ggcctgctgg gccacgtggg ccagttcatg cgccagcagc cggcggcccg
     1201 gcggcgtctc cggcgcgtac tgccccgcgg caaacaccag gtgcggtccc gtcgtgtagg
     1261 ccagggcgtc caggtcgcgc gccgattgcg cggcggcggc cccggtatgc acgcgcacgc
     1321 gggaaaagtc gtagccgaag cgcccctcca tgtcccggcg cagggccggg tccagcggcg
     1381 tgcccggccc agccagggcc gtggcgaccg agggcggggc gcccggcccg cccggtggct
     1441 cggtgaggcg ccggatgcgc gggggcgggt cgctcgcggc gggcggcccc ggcgtcgccg
     1501 tcacctgcgc ggcgaggcgg tcggcgtcct gctcgtagct gtcgtcgggg gcattcgtgg
     1561 cgagcttcgc ctgaaggtgg cccggctccg gcgcatggac ggccatgcgg ccaaagtcgt
     1621 gcccggcagg ccaacctctc ccccggcccc tccccgccgc ggggagggga gatgggctgg
     1681 gggccgggcg ggccggctcg gcggcggggt ggtgctgggg ctgcggcgcg tgggcaaatt
     1741 cgggcatgga ccaatccctg ctccgggggc aggggccgca cctgcgctgc cggggtggct
     1801 gttttcttcc gatactaata tcgcgcgttc gggggcgggg gatacggggt gccgctgaat
     1861 ttcaccgcgc agaacgcgaa gcgcgcgcag gctctacggg agtaatctca ccgcgaagga
     1921 acgaaggacg cgaagcatat gattaatccg aggtatctct gcgccctctg cgtctctgtg
     1981 gtgtaacccg attgcccggc acgcgcccga tccgctataa tcgcgctaag cagtttgggc
     2041 tagggttagc gcgggttggc ccccggatag gccggatagg atggaaggat gccgggtcgc
     2101 tttgcggggc cgctcctacc cgcgcgtgca gatcctgacg attgccgagc tcttgcgcgg
     2161 cgccgcgatc cagatgcccc cgcagttcgg cacgttccgc caggccccgc gcgtgg
//
LOCUS       DASUYC010000036         3088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_594912,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000036 DASUYC010000000
VERSION     DASUYC010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594912"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2971
                     /locus_tag="VF276_00530"
     CDS             <1..2971
                     /locus_tag="VF276_00530"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX6605376.1"
                     /translation="QVQYANRRARRWGADLLPAARHLGTTTREVELLVGRPGAEAQLV
                     RVTSSPLQVGGQVAGTCIVARPLPGYSRREAELAERNRALGALNDLAAGLLRLTTRAA
                     CYEAALDGVLAIVGARTGAFLCCDDAHQRFDLVALRGARDATRATLAALTYDTSGANN
                     LARTTGDLVILHAADPPQTGTSILIDEGVQTAVFLPVRAGEGVGAVLSYLLADEHDLT
                     PFEREILRTAATYIGLALERLALVEEAEDERARLAGILDALPVGVHIGAREADGAEHW
                     TPANAAARRQLTGQTLDPRQRGVVRRPDGQVFDFDDLPASRALVHGEASPPTELVITY
                     PDGATRLFLSSGSVLRDTGDRREAVVIAQEITALRAAEAALLQSEERFAKAFQASPDA
                     IIISRIADSLILDVNDHCLALFGYTRADMVGRRATEIGLWPDPATRAQLATRLAGEGA
                     LYDVEVPLRCKSGEIRLCSLSVAVLHLPGEPCILSHVRDITERRAGEDRLRHQLSFTQ
                     AITESLGEGVYAVDRAGHCTFVNPAAAQMLGWPAAELEGAPIHDRIHYQYADGTPRPA
                     LSCPILAVWREGTPVQVDDDVFTRRDGTTFPVAYNAAPIVTAGRVTGAVVVFRDVSEP
                     RAAQAQIARQLSHLTALRRIDVAIAGSRDLAATLYVVLDQVMLQMQVDAAAVLLREPG
                     RPMLTYAATRGFHTHPAAWPDLPAAAGYPAQAMRDRRALALPALAGAPGFERAALAAA
                     EGFQAYYVVPLIVRGAVLGALEVYQRAPRTFAPDWRDFLGTLAGQAAIAIDNARLFTD
                     LQRSNDELAAAYDTTLEGWARALDLRDHETQGHTQRVTERTLALARALGFSEPDLLHV
                     RRGALLHDIGKMGIPDAILLKPGPLTADERAVMRRHPVYAYELLAPIAFLRPALDIPY
                     AHHERWDGTGYPRGLRGSAIPLAARVFAVVDVWDALLSDRPYRPAWPAPEVRAYLRAH
                     AGTHFDPAVVAAFLAGE"
BASE COUNT          409 a         1349 c          926 g          404 t
ORIGIN      
        1 gcaggtgcaa tatgccaacc gccgcgcccg gcgctggggc gccgacctgc tgcccgccgc
       61 ccgccacctg ggcaccacca cccgcgaagt cgaactcctg gtcggccggc ccggcgccga
      121 ggcccaactg gtccgcgtca ccagcagccc cctccaggtc ggcggccagg tcgccggcac
      181 ctgcatcgtc gcccggcccc tgccgggcta cagccggcgc gaagccgaac tggccgaacg
      241 caaccgcgcc ctcggcgccc tcaacgacct ggccgccggg ttgctgcgcc tcaccacccg
      301 cgccgcctgc tacgaggccg ccctcgacgg cgtgctcgcc attgttggcg cccgcaccgg
      361 cgccttcctc tgctgcgacg acgcgcacca gcgcttcgac ctcgtggccc tgcgcggcgc
      421 ccgcgacgcc acccgcgcca ccctggccgc cctgacctac gacacctccg gcgccaacaa
      481 cctggcccgc accaccggcg acctggtgat cctccatgcc gccgatccgc cccagaccgg
      541 cacgagcatc ttgatcgatg aaggcgtcca gaccgccgtg ttcctgcccg tgcgggcggg
      601 cgagggcgtc ggcgccgtcc tgtcctacct gctcgccgac gagcacgacc tcaccccgtt
      661 cgagcgcgag atcttgcgca ccgccgccac ctatatcggc ctggccctcg aacgcctggc
      721 cctggtcgaa gaagccgagg acgagcgcgc ccgcctggcc ggcatcctcg acgccctgcc
      781 cgtcggcgtc catatcggcg cgcgcgaagc ggacggtgcc gagcactgga ccccggccaa
      841 cgccgccgcc cgccgccaac tgaccgggca aacgctcgac ccccgccagc gcggcgtggt
      901 ccgccgcccc gacggccagg tgttcgactt cgacgatctg cccgctagcc gcgccctggt
      961 gcacggcgaa gccagcccgc ccaccgaact cgtcatcacc taccccgacg gcgccacccg
     1021 gctgttcctc tccagcggca gcgtcttgcg cgacaccggc gaccgccgcg aggccgtcgt
     1081 catcgcccag gagatcaccg ccctgcgcgc cgccgaagcc gccctgctcc agtccgaaga
     1141 gcgcttcgcc aaggccttcc aggccagccc cgacgcgatc atcatcagcc gcatcgccga
     1201 cagcctgatc ctcgacgtga acgaccactg cctcgccctc ttcggctaca cccgcgcaga
     1261 catggtcggc cggcgcgcca ccgagatcgg cctctggccc gaccccgcta cccgcgccca
     1321 gttggccacg cgcctggccg gcgaaggcgc gctctacgac gtcgaagtgc cgttgcgctg
     1381 caaatcgggc gagatccggc tctgcagcct gtccgtcgcg gtcctccacc tgcccggcga
     1441 gccctgcatc ctctcgcacg tccgcgacat caccgagcgc cgcgccggcg aggaccgcct
     1501 gcgccaccag ctcagcttca cccaggccat caccgagagc ctcggcgaag gcgtctacgc
     1561 cgtcgaccgc gccggccatt gcaccttcgt caaccccgcc gccgcccaga tgctgggctg
     1621 gcccgccgcc gaactggagg gcgcccccat tcacgaccgc atccactacc aatacgccga
     1681 cggcacgccc cggcccgcgc tcagttgccc catcctcgcc gtctggcgcg agggcacgcc
     1741 ggtgcaggtc gacgacgatg tgttcacgcg ccgggacggc accaccttcc ccgtcgccta
     1801 taacgccgcg cccattgtca ccgcgggccg ggtcaccggc gcggtggtcg tcttccgtga
     1861 cgtgagcgag ccccgcgcgg cccaggccca gatcgcccgc caactgtcgc acctgaccgc
     1921 gctgcgccgc atcgacgtgg ccatcgccgg cagccgcgac ctggccgcca cgctctacgt
     1981 cgtgctcgac caggtgatgc tgcagatgca ggtcgatgcc gccgccgtgt tgctgcgcga
     2041 gccgggccgc cccatgctca cctatgccgc cacgcgcggc ttccacactc atcccgccgc
     2101 ctggcccgat ctccccgccg ccgccggcta ccccgcccag gccatgcgcg accgccgcgc
     2161 cctggccctg cccgccctgg ccggggcgcc cggcttcgag cgcgccgcgc tggccgccgc
     2221 cgagggcttc caggcctact acgtcgtgcc cctgatcgtg cgcggcgccg tgctgggcgc
     2281 gctcgaagtc tatcagcgcg cgccgcgcac cttcgccccc gactggcgcg acttcctggg
     2341 cacgctggcc ggccaggccg ccatcgccat cgacaacgcc cgcctcttca ccgacctgca
     2401 gcggtccaac gacgaactcg ccgccgccta cgacaccacg ctcgaaggct gggcgcgcgc
     2461 cctcgacctg cgcgaccacg agacccaggg ccacacccag cgcgtcaccg agcgcaccct
     2521 ggccctggcc cgcgccctcg gcttcagcga gcccgacctg ctgcacgtgc gccgcggcgc
     2581 cctgctgcac gacatcggca agatgggcat ccccgatgcc atcctgctca agcccggccc
     2641 gctcaccgcc gacgaacggg ccgtcatgcg ccgccacccg gtctacgcct acgaactgct
     2701 cgcgcctatc gccttcctgc gcccggcgct cgacatcccc tacgcccacc acgagcgctg
     2761 ggacggcacc ggctaccccc gcggcctgcg cggctccgcc atcccgctcg ccgcccgcgt
     2821 cttcgccgtg gtcgacgtct gggacgccct gctctccgac cggccctacc gccccgcctg
     2881 gcccgcgccc gaggtccgcg cctacctccg cgcccacgcc ggcacccact tcgaccccgc
     2941 cgtcgtcgcc gccttcctcg ccggcgagta gtcagaggtc agtggtcagt ggtcagtggt
     3001 cagtggtcag ccgtcagccg tcagccgtgt gctacgcacc gctgcgcgat caggagacga
     3061 gtgacgggtg ggccttgtca ttctgagc
//
LOCUS       DASUYC010000037         3451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_64801,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000037 DASUYC010000000
VERSION     DASUYC010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64801"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="VF276_00535"
     CDS             <1..574
                     /locus_tag="VF276_00535"
                     /inference="COORDINATES: protein motif:HMM:NF025049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX6605377.1"
                     /translation="EVGAGAYLGAAPMLQDKNLLGAAASIHNVEAQHASTLRAVMNDP
                     APSPAFGSPLTVDQVLAAVTPLLKPNSAPPAGKYYTNMDPAPNITVATQPVAQVANSA
                     GLVYFAETQHTAGGPFLDYWTRHGGLVQLGFPITEPYKGPSPTNGQEYVQQYFQRARL
                     EWHPEHKGTEFEVQLGLLGAEQLFHDMMGR"
     gene            669..995
                     /locus_tag="VF276_00540"
     CDS             669..995
                     /locus_tag="VF276_00540"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX6605378.1"
                     /translation="MEIFGMGPGEMLIILVLALIIVGPQKLPELGRNLGRTIGEFKAQ
                     TDSLRSVMTFEPAPPSSTTPVKVPHPAERQDERVSGLLAYSNRTVEQHTTTASEAVAT
                     DEQRAS"
     gene            1025..1186
                     /locus_tag="VF276_00545"
     CDS             1025..1186
                     /locus_tag="VF276_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX6605379.1"
                     /translation="MFGLGAPELLLIMGIVIIIFGAGKLPEVGAAMGKSIREFRSATE
                     EPAKKDTVA"
     gene            1277..2044
                     /gene="tatC"
                     /locus_tag="VF276_00550"
     CDS             1277..2044
                     /gene="tatC"
                     /locus_tag="VF276_00550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX6605380.1"
                     /translation="MSVLTWKRRATPPGKSYGPGATMSLVAHLEELRDRLIRATLAVV
                     VTTTLAFVFINSIMSWLLDLAGNHTIQALDPTETFATYFKVAFTAGLGLAMPVLIYQL
                     LRFLAPGLTRGERRALYLSLPFVVVCFVTGALFCYFIILPSALNFLLGFGDPRILKQI
                     SLTKFIGFVSNFILAIGAAFELPVFIFMLAKLGIASYSRLARFRKYALLLAFVVAAII
                     TPTPDPINQALVAVPLFLLYELGLQLSRFARKPRTAA"
     gene            2189..2809
                     /locus_tag="VF276_00555"
     CDS             2189..2809
                     /locus_tag="VF276_00555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6605381.1"
                     /translation="MDSRDPATLPSDAELMAAVRSRNAGALQQLYDRHFRRAYAVAFR
                     MLGEAGAAEDCVHDQFLKLWQKPDLYDPRRGVFIYWFLATVHNNASNYLRRQGRTRPL
                     GNPLSEDEPGLTPEPADRDAGQSSVEDLAHQAEAQAVVRAALAELTSQQRAVLELAYF
                     GGMSQSEIAQYLQEPLGTVKTRIRTGMIKLRAALETRGWGKDMGFQ"
     gene            2806..>3451
                     /locus_tag="VF276_00560"
     CDS             2806..>3451
                     /locus_tag="VF276_00560"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX6605382.1"
                     /translation="MNPSMNGGEDLFREQLAAYALGALDPDEAAALEAHLPTCAACRA
                     ELAEFESVVPALAFSLDVPLEPAGHRERFLNLLNEAPEPAPARPAPAPAAPVVAEPRS
                     VAAPRRPWWQALNPFAGWAAAGLMAVLLIAALLWGAGAQQRATDAERTVVAQQARSQA
                     MAAILANPNARRVTLAGQGAFQDAGVQLWIDPASGRAVLVSGNLAAAPVGKLYEL"
BASE COUNT          572 a         1200 c         1127 g          552 t
ORIGIN      
        1 ggaagtgggg gctggggcct acctgggcgc ggcccccatg ctccaggaca agaacctgct
       61 gggcgccgcc gcgagcattc acaatgtgga agcccagcac gcctcgaccc tgcgcgccgt
      121 gatgaacgac ccggctccgt cgcccgcgtt cggctcgccg ctgacggtgg accaggtgct
      181 ggccgccgtg acgccgctgc tcaagccgaa cagcgcgcca cccgccggca aatactacac
      241 caacatggac ccggcgccga acatcaccgt ggcaacccag cccgtcgccc aggtggcgaa
      301 cagcgccggc ctggtctact tcgcggagac gcagcacacc gcgggggggc ctttcctcga
      361 ctactggacc cgccacggcg gcctggtcca actcggcttc cccatcaccg agccgtacaa
      421 ggggccgagc ccgaccaacg ggcaggaata tgtgcagcag tacttccagc gcgcccgcct
      481 cgaatggcac cccgagcaca aggggaccga gttcgaggtg cagttgggcc tgctgggcgc
      541 cgagcagctc ttccacgaca tgatgggccg ctaaccggcg cgcatcccgg cctgccgggg
      601 cgggaacggg ccagtgggcc cggctctgcc ccggcaccgg gagtacccca tcgcagggag
      661 ggttaacgat ggaaatcttt ggcatgggcc ccggcgagat gctgatcatc ctggtcctgg
      721 cgctgattat cgtgggaccg cagaagctgc ccgaactggg ccgcaacctg ggccgcacca
      781 tcggcgagtt caaggcgcag accgattcgc tgcgtagcgt gatgaccttt gagccggccc
      841 cgccgtctag cacgacgccg gtaaaggtgc cccaccccgc cgagcggcag gacgagcgcg
      901 tgagcggcct gctggcctac agcaaccgga ccgtggaaca acacacgaca accgcctcgg
      961 aggcggtcgc taccgacgaa caacgcgcct cctgagcgga ggcggcatcg agaggagaca
     1021 gaccatgttt ggattaggcg cccctgagct attgctcatc atgggcatcg tgatcatcat
     1081 tttcggcgcg ggcaagctgc cggaggtcgg cgcggccatg ggtaagagca tccgcgagtt
     1141 ccgcagcgcg accgaggaac cggccaagaa agataccgtg gcctagtgcg gtgccgcccg
     1201 cggctgccaa ccccacccgc gggcgcccgt tccccggccc ggcccatgca ggccgggcgg
     1261 cgagaggagt tatgtaatga gtgttcttac ctggaagcgc agggcgaccc cgcccggcaa
     1321 gagctacggc cccggcgcga ccatgtcgct ggtcgcgcat ctggaggaac tgcgcgaccg
     1381 gctgatccgc gccaccctgg cggtggtggt gacgactacg ctggccttcg tgttcatcaa
     1441 cagcattatg agctggttgc tcgacctggc cggcaaccac acaatccagg cgctcgaccc
     1501 gaccgagacc tttgccacct atttcaaggt cgcctttacc gccgggctgg gcctggcgat
     1561 gcccgtgctg atctaccagt tattgcgctt cctggcaccg ggcctgacgc gcggcgaacg
     1621 gcgggcgctc tacctgagcc tgcccttcgt ggtcgtctgc ttcgtgaccg gcgcgctgtt
     1681 ctgctacttc atcattctgc cgtcggcgct gaacttcctg ctgggattcg gcgacccgcg
     1741 catcttgaag cagatctccc tgaccaagtt catcggcttt gtgagcaact tcatcctggc
     1801 catcggcgcg gccttcgagt tgccggtgtt tatatttatg ctggcgaaac tgggcatcgc
     1861 cagttatagc cggctggccc gcttccgcaa gtatgccttg ttgctggcgt ttgtcgtcgc
     1921 ggccattatc acccccaccc ccgatccgat caaccaggcg ctcgtcgccg tgccgctgtt
     1981 cctgctctat gaactcgggc tgcaactgag ccgcttcgcc cgcaaaccgc gcaccgcggc
     2041 ctgacgttga ctggaaggat gggcacattt catgctatgc acctactcgc cgccaggaag
     2101 cccagccggc ttccgctatc acgatgcacg ccgggccccc gccggtggtc gggtggatgt
     2161 gccagcacga gattcggaga aggccctatt ggatagtcga gacccggcca ccctccccag
     2221 cgatgccgaa ttgatggcag ctgtgcgcag ccggaatgcc ggcgcgttgc agcagcttta
     2281 tgatcgccat ttccggcgcg cctatgcggt cgccttccgc atgctgggcg aagccggggc
     2341 cgccgaagac tgcgtgcacg accagttcct caagctctgg caaaagccgg acctgtacga
     2401 tccccggcgc ggcgtcttta tctactggtt tctggcgacg gtgcataaca acgccagtaa
     2461 ctacctgcgc cggcagggcc gcacccgccc gttgggcaat ccgctcagcg aagacgagcc
     2521 tgggctgacc cccgaacccg ccgaccgcga cgcggggcag tcgagtgtgg aagatctggc
     2581 gcaccaggcc gaggcgcagg ccgtggtgcg cgccgccctg gccgagttga ccagccagca
     2641 acgcgcggtg ctggaactcg cctatttcgg cggcatgagc caatcggaga tcgcccagta
     2701 tttgcaggag ccgttgggca cggtgaaaac acgtatccgc acggggatga tcaaattgcg
     2761 cgcagcgctg gaaacccgtg gatggggaaa ggacatgggt ttccaatgaa cccgagcatg
     2821 aacggcggcg aggatctctt ccgggagcag ctcgcggcgt atgccctggg cgccctcgac
     2881 ccggacgaag ccgcggcgct ggaggcccat ctgcccacct gcgcggcgtg ccgggcggaa
     2941 ctggccgagt tcgagtcggt cgtgcccgcg ctggccttca gcctcgacgt gccgctggag
     3001 ccggcggggc accgcgagcg cttcctcaac ctgctgaacg aagcgcccga gccggcgccg
     3061 gcccggcccg cgcctgcacc tgccgcccct gtcgtggccg agccgcgctc ggtggccgcg
     3121 ccgcgccgcc cctggtggca ggcgctcaac ccgttcgcgg gctgggccgc cgccgggctg
     3181 atggccgtgc tgctcatcgc ggcgctgcta tggggcgcag gggcccagca gcgcgccacg
     3241 gacgccgagc gcacagtagt tgcccagcag gcgcgcagcc aggccatggc cgccatcctc
     3301 gccaatccca atgcccgccg ggtaaccctg gccggtcagg gcgcattcca ggatgcgggg
     3361 gtgcaactct ggatcgatcc ggccagcggg cgcgccgtcc tggtcagcgg caatctagcc
     3421 gccgccccgg tgggcaaact ctacgaactc t
//
LOCUS       DASUYC010000038         4891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1939963, whole genome shotgun sequence.
ACCESSION   DASUYC010000038 DASUYC010000000
VERSION     DASUYC010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4891
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1939963"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..1011
                     /locus_tag="VF276_00565"
     CDS             49..1011
                     /locus_tag="VF276_00565"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6605383.1"
                     /translation="MAGIAIGQPWAEGGFKNLRIEGYTPDAAHLAALTRVVNATQGTD
                     WPVEQVAQYYGHPAFELERDARLVWLGDKPVAAAICYPTIHLHDRAPGNFEIFVVPEA
                     RGHGLGSRLLAHLEQAARDRGHHVLETTIDQHDAPGRSFLLAHGFRVIGQAAHLVRAG
                     MADLPPVDLPEGFSISNLADAPDAGEDYRALANRLGAYDPGYNLIDPEELAGLTASAA
                     WEPEGIFVLADPQDREVGVIRASGAGRDHGYLHEIRIDPAYRGRNLGTALVGTALAYL
                     ATNGVQQTELDSEGPDTPPYRLARRCGFVERHRWQQLLKPLARP"
     gene            1016..3028
                     /locus_tag="VF276_00570"
     CDS             1016..3028
                     /locus_tag="VF276_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605384.1"
                     /translation="MRFSRILAPFRGPHLYGLLALALLLIALVYQVGGDYAWDMGNPR
                     ADVYLGNYFGVERAGDVRFRWTQPVSRLQAPLVGSYNGRVRVRLNGDTFGGPPRQATI
                     RVGATLTRTVALEPGWHDYELAFTRADLWPGDLQVWLFESELQIPEGQRDPADTRRLG
                     VPVQAITVERADRLVLPPLGMLAGTALAVVLLFALAVWFGVPRRAASGITALLILGWA
                     AALFVGRLPAVFAAPGLLGTLALGAGMVLLLRPLLGRLFRRGGIAVRPVEWQVLAGLF
                     VLGFVFKASGLLDPMFTTLDHYARLHRLMEMGDDPVYFLANFLSSDKGQTYAGQLGLT
                     AVIPYSPLFYIAFDPLNWLVPDPNARLQAINLVCSALEASNVFFLFYTLKRAWGDGLA
                     GLWAGALFVAFPLSNLLFSDGGYPGIFANWLLVLTIAALAWGYPRIGRPQVLIPLAIL
                     LGLALLAHTANAILLAPTLVLFALAVARWDRARLRGVVLWAGGGLALAFLSFYQFSAG
                     HVVTDVLPQIVDKLRKIGGVGKGADHLGAPLLSGFGPQIEAHFRTWPVLLAVPALWRA
                     AHPAPGAQVERDPAARGLALWLGASAITFAVFSVLDLWINLLQKHMIYFMPALALLSG
                     WSLAAIWRRGGAARWLCLALWAVLFAASLGVWVNRVVNYVLPPGSG"
     gene            3151..4599
                     /gene="glnA"
                     /locus_tag="VF276_00575"
     CDS             3151..4599
                     /gene="glnA"
                     /locus_tag="VF276_00575"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX6605385.1"
                     /translation="MTTEQTTRKPLYANGEQSPAIIQETLRRADEQGVRFVNLQFTDL
                     MGIVKSVTIPIHQLEDSATNGKWFDGSSIEGFARIAESDMFLIPDLRTFAVLPWERDG
                     IGAPSTNGNGKLNGHGPAHGTTTARIICWIYTPNGELFPGDPRLVLARAMDYAANLGY
                     RYQTGPELEFYLFKPENGRSTTATHDMGGYFDLTGDEGTLVRKDMVNALEAMGIKVET
                     SHHEVGPGQHEIDFEYDTALTTADSAVTFKYTLKAVADQHGLYATFMPKPVFGIAGSG
                     MHTHQSLADIDNGKNLFGSADDAYNLTPVAKHFIAGQLRHARAMTAILNPLVNSYKRL
                     VPGYEAPVYMGWARQNRSALIRVPRINPTKPQAARVELRSPDPTCNPYLAFAVMLRAG
                     LEGIEQGLNAPEPVDENLYHFDEHERQRRNIGVLPGSLDEALNEMEKDDLIRETLGDH
                     VFERYLEAKRQEWDDFRTRVTPWEIERYLETY"
BASE COUNT          768 a         1740 c         1581 g          802 t
ORIGIN      
        1 ggctgacggc tgacggctga cggctgccag ctaaggagga accaaacgat ggcgggaatc
       61 gcaatcggcc aaccgtgggc ggagggcggg tttaagaacc tgcgcatcga ggggtatacc
      121 ccggatgcgg cgcatctggc cgcccttacc cgcgtggtga acgccacgca gggcacggac
      181 tggccggtgg aacaggtggc gcagtattac gggcacccgg ctttcgagct ggagcgcgac
      241 gcgcgcctcg tgtggctcgg cgacaagccc gtggccgccg ccatctgcta cccgaccatc
      301 cacttgcacg accgtgcgcc cggcaacttc gagatcttcg ttgtgcccga agcgcggggg
      361 cacggcctgg gcagccgcct gctggcccac ctcgaacagg ccgcgcgcga ccgcgggcac
      421 catgtcctgg agacgaccat cgaccagcac gacgcgcctg ggcgcagctt tttgctggcg
      481 cacggtttcc gcgtcatcgg ccaggccgcc cacctggtgc gcgcgggcat ggccgatctg
      541 cccccggttg atctgccgga gggcttcagt atcagcaacc tggccgatgc gccggatgcg
      601 ggcgaggact atcgggcgct cgccaaccgc ctgggcgcct acgatcccgg ctacaacctg
      661 atcgaccccg aagaactggc gggcctgacc gcgagcgccg cgtgggagcc ggagggcatc
      721 ttcgtgctgg ccgatccgca ggaccgcgag gtcggcgtca tccgcgcgtc gggcgcgggg
      781 cgggatcatg gctacctgca cgagatccgc atcgacccgg cctatcgcgg gcgcaacctg
      841 ggcaccgcgc tggtcggcac ggcgctggct tacctcgcca cgaacggggt ccagcaaacc
      901 gaactcgata gcgaaggccc cgacacgccg ccctatcgac tcgcccggcg ctgcggcttc
      961 gttgagcgcc accgctggca gcaattgctc aagccgctgg cccgcccctg atcgcgtgcg
     1021 tttctcccgc atcctcgcgc ccttccgcgg cccccacctc tacggcctgc tcgcattggc
     1081 cctgctgctg atcgcgctgg tctaccaggt gggcggcgac tacgcctggg atatgggcaa
     1141 cccgcgcgcc gatgtctacc tgggcaacta tttcggcgtc gagcgcgcgg gcgacgtgcg
     1201 cttccgctgg acacagccgg tgtcacggct gcaggcgccg ctggtgggca gctataacgg
     1261 gcgcgtgcgc gtgcggctca acggcgacac cttcggcggc ccgccccggc aggccacgat
     1321 ccgcgtcggc gccaccctca cccgcaccgt ggccctcgaa cccggctggc acgactacga
     1381 gttagccttc acccgcgccg atctctggcc cggcgatctc caggtctggc tgttcgagag
     1441 cgagttgcag atccccgaag gccagcgcga cccggcggac acccgccgcc tgggcgtgcc
     1501 ggtgcaggcc atcacggtcg agcgcgccga ccggctggtg ctcccgccgc tgggcatgct
     1561 ggccgggacc gcgctggccg ttgtgctgct ctttgccctg gccgtctggt tcggcgtgcc
     1621 gcgccgggcg gccagcggga tcaccgccct gctgatcctg ggctgggcgg cggcgctatt
     1681 cgtgggccgc ctgcccgccg tcttcgccgc gccgggcttg ctggggacac tggcgctggg
     1741 tgcggggatg gtgttgctac tgcgcccgct gctgggccgg ctgttccggc gcggcggcat
     1801 cgccgtgcgc ccggtcgagt ggcaggtgct ggccgggctg ttcgtgctcg gcttcgtctt
     1861 caaggcgagc gggctgctcg acccgatgtt caccacgctc gaccactacg cgcgcctgca
     1921 ccggctgatg gagatgggcg acgacccggt ctatttcctc gccaacttcc tgagcagcga
     1981 caagggccag acctatgccg ggcaactcgg cctcaccgcc gtcatcccct attcgcccct
     2041 gttctacatc gcctttgacc cgctgaactg gctggtgccc gacccgaacg cccgcctgca
     2101 ggccatcaat ctggtgtgca gcgccctgga agcgagcaac gtcttcttcc tgttctacac
     2161 gctcaagcgc gcctggggcg acggcctggc tgggctctgg gccggagcgc tgttcgtggc
     2221 cttcccgctc agcaacctgt tgttctcgga cggcggctac cccggcatct tcgccaactg
     2281 gctgctggtg ctgaccatcg ccgcgctggc ctggggctat ccccgcatcg ggcgccccca
     2341 ggtgctgatc ccgctcgcca tcctgctcgg cctggcgctg ctggcgcaca ccgccaacgc
     2401 catcctgctg gcgcccacgc tggtgctgtt cgcgctggcc gtggcgcggt gggaccgggc
     2461 gcggctgcgc ggtgtggtcc tctgggcggg cggcgggttg gccctggcct tcctgagctt
     2521 ctaccaattc agcgcgggcc atgtcgtcac cgacgtgctg ccgcagatcg tggacaagct
     2581 gcgcaagatc ggcggcgtcg gcaaaggcgc cgaccacctg ggcgcgccgc tgctgagcgg
     2641 cttcgggccg cagatcgaag cccatttccg cacctggccg gtgctgctgg ctgtccccgc
     2701 cctctggcgc gcggcacatc ctgcgcccgg cgctcaggtc gagcgcgacc cggcggcgcg
     2761 cggcctggcg ctctggctcg gcgcgtcggc gatcacattc gccgtgttca gcgtgctcga
     2821 cctgtggatc aacctgttgc aaaagcacat gatctacttc atgcccgcac tggcgctact
     2881 gagcggctgg agtctggccg cgatctggcg ccggggcggg gcggcgcgct ggctctgcct
     2941 cgcgctctgg gccgtgctgt tcgcggcgag cctgggggtc tgggttaacc gggtggtaaa
     3001 ctatgtgctg ccgcccggct ccggatgagc gaaatcttag gcataatgca caaaacggcg
     3061 gggggatgtt tgttacggct gctctccggc agtacaccaa aacatgttat gctaaccgtg
     3121 tcgattttcc accccgatta ggaggcatca atgaccaccg agcaaacgac ccgcaagccc
     3181 ctgtatgcca acggcgaaca atctcccgcc atcatccagg aaaccctgcg ccgcgccgac
     3241 gaacaaggcg tgcgctttgt gaacctccag ttcaccgacc tgatgggcat cgtcaagagc
     3301 gtgaccatcc cgatccatca gttggaagac tcggccacca acggcaagtg gttcgacggt
     3361 tcctccatcg aaggcttcgc gcgcatcgcc gagtcggata tgttcctgat tcccgacctg
     3421 cgcaccttcg ccgtgctgcc ctgggagcgc gacggcatcg gcgcccccag caccaacggc
     3481 aacggcaagc tcaacggcca cggcccggcc cacggcacga ccacggcccg cattatctgc
     3541 tggatctaca cgcccaacgg cgagctgttc cccggcgacc cgcgcctggt gctggcccgc
     3601 gccatggact acgccgccaa cctgggctat cgctaccaga ccggcccgga actggagttc
     3661 tatctcttca agcccgagaa cggccgcagc accaccgcga cccatgacat gggcggctac
     3721 ttcgacctga ccggcgacga gggcaccctg gtgcgcaagg atatggtcaa cgcgctcgaa
     3781 gccatgggca tcaaggtcga gacttcgcac cacgaggtcg gtcccggcca gcatgagatc
     3841 gacttcgagt acgacaccgc cctgaccacc gcggacagcg cagtcacctt caagtacacg
     3901 ctgaaagccg tcgccgacca gcacggcctg tacgccacct tcatgcccaa gccggtcttc
     3961 ggcatcgccg gcagcggcat gcacacgcac cagtcgctgg ccgacatcga caacggcaag
     4021 aacctcttcg gcagcgccga cgatgcctac aacctgacgc ctgtcgccaa gcacttcatc
     4081 gccgggcagt tgcgccacgc gcgcgccatg accgcgatcc tgaacccgct ggtgaactcg
     4141 tataagcgcc tggtgcccgg ctatgaggcc ccggtctaca tgggctgggc gcgccagaac
     4201 cgctcggcgc tgatccgcgt gccgcgcatc aacccgacca agccgcaggc cgcccgcgtc
     4261 gaactgcgca gcccggaccc gacctgcaac ccgtacctgg ccttcgccgt catgctgcgc
     4321 gcgggcctgg aaggcatcga gcagggcctg aacgcgcccg agccggtgga cgagaacctg
     4381 taccacttcg acgagcacga gcgccagcgc cgcaacatcg gcgtgctgcc cggcagcctg
     4441 gacgaggccc tcaacgagat ggagaaggac gacctgatcc gcgagacgct gggcgaccac
     4501 gtgttcgagc gctacctgga agccaagcgc caggagtggg acgacttccg cacgcgcgtc
     4561 accccgtggg agatcgagcg ctacctcgaa acctactaag cccgccccgg cggcatataa
     4621 gctgcattcc gacccgcatc gccctcaccc ggcggtgcgg gttttttagc tgtcagccgt
     4681 cagccgtcag gtgtcaggta tcagataaat agcacacagt gtggatttgg taaattaggc
     4741 cacgatgcag ccggtgtcac tctgagcgca gcgaagagtc tcgtcgcgtg accaatgaga
     4801 cgagattctt cgctgcgctc agaatgactg accccggtcg tcaggctata gagttgacca
     4861 ggcgcgcggg atatgacatg cttttcagag a
//
LOCUS       DASUYC010000039         3383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1319611, whole genome shotgun sequence.
ACCESSION   DASUYC010000039 DASUYC010000000
VERSION     DASUYC010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319611"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VF276_00580"
     CDS             <1..550
                     /locus_tag="VF276_00580"
                     /inference="COORDINATES: protein motif:HMM:NF014177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEX6605386.1"
                     /translation="ISGTYGAFLKGWDPRALKPLTLRPPAILPSTQPHAAHCTDATTL
                     VTQQPCDVLYLDPPYNSRPYAGNYHLLETIARYDAPPVYGKTGLRPAAARSPYNRAGH
                     AATALRTLVRGARCTHLLLSYNSEGLIPETAILDILGERGRPEVRRRPSRRFRSDADS
                     SRRRYAPQHEVSENLYYVRCGP"
     gene            547..1398
                     /locus_tag="VF276_00585"
     CDS             547..1398
                     /locus_tag="VF276_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEX6605387.1"
                     /translation="MSEPAPIDPRNRLNDLTGREWVYFLQSVQATAYPARGPESYGHN
                     LRRAHPSPKPPQLMADIIRFFTKAGGWVLDPFMGVGGTLLGCALTGRHGAGVDLSPDY
                     LAIYRQVCAQEGLAEQLTVCGDARDLDRYPAVRDHVFDLILTDPPYGDMLARPQDGEK
                     KKRTGSDAPTPFTAQPEDLGNLPYDAFLDALDGILAGAVRYLKPGGYLVVFAKDLQPT
                     ATHHNMLHADLAARLPAAIPGLRFRGYKIWVDQTVRLYPFGYPFQFVANQLHQYILVF
                     QAPAGPR"
     gene            complement(1395..2399)
                     /locus_tag="VF276_00590"
     CDS             complement(1395..2399)
                     /locus_tag="VF276_00590"
                     /inference="COORDINATES: protein motif:HMM:NF020448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HEX6605388.1"
                     /translation="MLHITNGGSAAGGLREAGLPGTVLAWNDVLHEGPVPAGLIPEAL
                     RDVRVRFLAASGWATYESALAEFTRRDDALARAADQDEVVLWFEHDLYDQLQLLQILD
                     RLARLAPGPTRFSLIAIDHFPGVVPFHGLGQLAPGQLASLYPTRRRLTAPALALGQAA
                     WHAFTAPDPAAITDLLAGDTSPLPFLRDALLRHLEQFPAVRDGLARTERQILQALAAG
                     AQGPAEIFQAGQQQEARPFMGDSVLWSYLLRLGKEPQPLVARTDGARFLLPLAFGRRA
                     DFLAQRLRLTPHGAAVLAGTADAIRGRGIDHWLGGVRLWGADAPWRWDAQAGRVVAAA
                     "
     gene            complement(2631..>3383)
                     /locus_tag="VF276_00595"
     CDS             complement(2631..>3383)
                     /locus_tag="VF276_00595"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX6605389.1"
                     /translation="LAPGQKVAALMGQMGRAYDGSYAEYTLVPASQVMPLTTDLPWET
                     LAAIPETYLTAWGSLVEALDVQAGQPLLIRGGTSALGMAAISLGKDMGLYVAATTRDR
                     AKAPALVANGADDVLIDSGRLAQAARARIPEGFDCTLDLIGTVTLLDSLGTVRPRGIV
                     CMSGLLGNAWTLHEFEPLAQMPSTVRLTIYDSETLTAANGTAALQRIVDGVAAGRYQP
                     NLHKVFRLDEIAAAHRYMEENRGTGKLVVVTE"
BASE COUNT          512 a         1245 c         1144 g          482 t
ORIGIN      
        1 catctccggc acctacggcg cgttcctcaa gggctgggac ccccgcgccc tgaagccgct
       61 gaccctccgc ccgcccgcca tcctgcccag cacgcagcct catgccgcgc attgcaccga
      121 cgcaaccacg ctggtcacgc agcagccctg cgatgtgctc tacctggacc cgccctacaa
      181 cagccgcccc tatgccggga actaccacct gctggagaca atcgcgcgct acgacgcgcc
      241 gcccgtctat ggcaagacgg ggctgcggcc cgccgcggcc cgctcgccct ataaccgcgc
      301 cgggcacgcg gccacggccc tgcgcacgct ggtgcgcggc gcgcgttgca cccacctgct
      361 gctcagctac aacagcgaag ggctgatccc cgaaaccgcc atcctcgaca tcctgggcga
      421 gcgcgggcga cccgaggtgc gccgccggcc ctctcgccgc ttccgcagcg acgccgacag
      481 cagccgccgc cgctacgccc cgcagcacga ggtgagcgaa aacctgtact acgtgcggtg
      541 cggcccatga gcgaaccggc gcccatcgat ccgcgcaacc ggctgaatga cctgaccggg
      601 cgcgagtggg tctacttcct gcaatcggtg caggcgacgg cgtacccggc gcgcgggccc
      661 gagtcctacg gtcacaacct gcgccgggcg cacccctcgc cgaagccgcc gcaactcatg
      721 gccgacatca tccgcttctt caccaaggcg ggcggctggg tgctcgatcc gttcatgggc
      781 gtgggcggga cgctgctggg ctgcgcgctt accgggcggc atggcgccgg cgtggacctg
      841 tcgcccgact acctggcgat ctaccggcag gtctgcgcgc aggagggact ggccgagcaa
      901 ctgaccgtct gcggcgatgc ccgtgacctg gatcgctatc ccgccgtccg cgatcacgtt
      961 ttcgacctga tcctgaccga cccgccgtac ggcgacatgc tggcccggcc ccaggacggc
     1021 gagaaaaaga agcgcaccgg gtcggacgcg cccacgccgt ttaccgcgca gcccgaagac
     1081 ctgggcaacc tgccgtatga cgcctttctg gacgcgctcg acggcatcct ggcgggcgcg
     1141 gtgcgctacc tcaagccggg cggttacctg gtcgtctttg ccaaggactt gcagccgacg
     1201 gccacgcacc acaacatgct gcacgccgac ttggccgccc gcctgcccgc cgcgatcccc
     1261 ggcctgcgct ttcgcggcta caagatctgg gtcgatcaga cggtgcgcct ctaccccttc
     1321 ggctacccgt tccagttcgt cgccaaccaa ctccaccagt acatcctggt gtttcaggcg
     1381 cccgccgggc cgcgctaagc ggcggcgacc acccgcccgg cctgcgcatc ccagcgccag
     1441 ggggcgtccg ccccccacag gcgcaccccg cccagccagt ggtcgatgcc ccggccccgg
     1501 atggcgtcgg cggtcccggc gaggacggcg gcgccgtgcg gggtgaggcg caggcgttgg
     1561 gccaggaagt cggcacgacg cccgaaggcg agcggcaaca ggaagcgcgc gccatccgtg
     1621 cgggcaacca gcggctgcgg ctccttgccg aggcgtagaa ggtagctcca gaggaccgaa
     1681 tcgcccatga aggggcgcgc ctcctgttgc tggccggcct ggaagatctc ggcggggccc
     1741 tgcgcgcccg cggcaagggc ctgcaggatc tgccgctcgg tgcgggcgag gccgtcgcgc
     1801 accgcgggga attgctccag gtggcgcagc agggcgtcgc gcaggaaggg cagggggctg
     1861 gtatccccgg ccagcagatc ggtgatcgcg gcggggtcgg gcgcggtgaa ggcgtgccag
     1921 gcggcctggc ccagggccag cgccggggcc gtgaggcggc ggcgcgtggg gtacaggctc
     1981 gccagttggc cgggcgccag ttggcccagg ccgtggaagg gcacgacacc gggaaagtgg
     2041 tcgatggcga tcaggctgaa gcgggtggga ccgggcgcca ggcgcgccag ccggtcgagg
     2101 atttgcagca gttgtaactg gtcgtagaga tcgtgctcga accagagcac gacctcgtcc
     2161 tggtcggcgg cgcgggccag ggcatcgtcg cgccgcgtga actcggccag ggccgattcg
     2221 taggtcgccc agccggacgc cgccaggaag cgaacgcgca catcgcgcaa ggcctccggg
     2281 atcagccccg ccggcaccgg cccttcgtgc agcacatcgt tccaggccag cacggtgccg
     2341 gggaggcccg cctcccgcag cccacccgcc gccgatccgc cgttcgtgat gtgcaacatc
     2401 gtcccgctcc tccggcgtcc gctcgattcc ccaccgctac gattccctac agtgtagcac
     2461 ggatgttcgc gctgcacaag ccgagtgtgg catcaattca agtacaccgc agagacgcag
     2521 aggccgcaga gatcattaaa ataggtcctg tttatgatcc gaaggccatt aacgcagccc
     2581 gccgccgtgc ccattatctc ggcgcatggg cgcttagctt cgttgctggc ctactccgtc
     2641 accaccacca gctttcccgt gccgcggttc tcctccatgt agcggtgggc cgcggcgatc
     2701 tcgtccaggc ggaagacctt gtgcagattg ggctggtaac gccccgccgc cacgccgtcg
     2761 acgatgcgtt gcagcgccgc cgtgccgttg gccgcggtca gcgtctcgct gtcgtagatc
     2821 gtcaggcgca cggtgctggg catctgcgcc agcggctcaa actcgtgcag cgtccaggcg
     2881 ttgcccagca ggccggacat gcagacgatg ccccgcgggc gcaccgtacc cagcgagtcg
     2941 agcagcgtca ccgtgccgat caggtccagc gtgcagtcga agccctcggg gatgcgggcg
     3001 cgcgccgcct gggcaagccg gcccgagtcg atgagcacgt cgtcggcgcc gttggcgacc
     3061 agcgcggggg ccttggcgcg gtcgcgggtc gtcgccgcca cgtaaagccc catgtctttg
     3121 cccaggctga tcgccgccat gcccagcgcc gaggtgccgc cgcggatcag caacggctgc
     3181 ccggcctgca cgtcgagcgc ctcgaccagc gacccccagg cggtcaggta ggtttcgggg
     3241 atcgccgcca gcgtctccca gggcagatcg gttgtcagcg gcatgacctg gcttgccggg
     3301 acgagggtgt actcggcgta gctgccgtca taggcgcgcc ccatctggcc catcagcgcc
     3361 gcgaccttct gccccggcgc taa
//
LOCUS       DASUYC010000040         6147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1356459, whole genome shotgun sequence.
ACCESSION   DASUYC010000040 DASUYC010000000
VERSION     DASUYC010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6147
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1356459"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="VF276_00600"
     CDS             complement(<1..872)
                     /locus_tag="VF276_00600"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX6605390.1"
                     /translation="MLLIYVAVWMAVALAIFGLTGSIVLLVVQPQRRVYSRVRQFAAT
                     AEAATPAQETQVRKDQRAALFAELDARWASGVKAKDLSLDLSRAEWHLTLTEFALIRA
                     ATAFGLTVLLWLLAPGVWWLSLLPALFIGWRLPLAYLHWSVSRRIRRLDSQLADILNI
                     LAGAVRSGSSLFQALDRIAREAPEPSKTEYTRVMRAISLGAPLDQALRRLAERVPTED
                     MEILVTAITIQQQTGGNLAHILDLIATTVRERHRIQREIQVLSAQQRLSAWLLTALPI
                     GMVGLLYLINPDYIG"
     gene            complement(903..2495)
                     /locus_tag="VF276_00605"
     CDS             complement(903..2495)
                     /locus_tag="VF276_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEX6605391.1"
                     /translation="MPIIPSKSNSLTPPGEPAAPAAPSSTPLDSVSVPSPYRPLSGAP
                     LAGSGEARPASTNGAAGGRPGSGPLNPAVVGARPGTGPLNATVLGPPTSSRTISAMLS
                     RPNAVDLQHRLHSKIIGELRDSVDLNDAVGLRQEVERLFNRFMAEEDVPLNRSERGRL
                     FELVCAEIMGYGPIQQLINSDAVNEIMVNGPHQVWVERQGKITLTDINFKDDEHVMRI
                     ISRIVAPLGRRVDESSPMVDARLPDGSRVHAIIPPLSLSGPILTIRKFARVPLTVEDL
                     MNLGALSQQAVIFLEAAVRAGLNVLVAGGTSSGKTTLLNVLSSFIPSEERIITIEDAA
                     ELQLQQVHVIRLESRPQSMERQNSVTIRDLVVTSLRMRPDRIIVGECRDAEALDMLQA
                     MNTGHDGCMTTVHSNSPRDAISRIETMSMMARAELPLRAIREQISAAFDLIVYMERLD
                     DGTRKVTYVSEVQGFEQDTILMQDVFRREWVQRGDQQVYDLIPTGIRPQVMRKLEKKR
                     IFLAPDFFVVPIAPGLPARNGR"
     gene            complement(2525..3775)
                     /locus_tag="VF276_00610"
     CDS             complement(2525..3775)
                     /locus_tag="VF276_00610"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6605392.1"
                     /translation="MTQLPDYTINVVVGDADADELARTRAVLLQGPKIRVTGTARAHA
                     EVLALAELEPDIFLLSTNLDPAGMPALISQVLDVAPASQVILVTDFGDPLDIGQAMVA
                     GARGLVRKPLAAHDVVGTIQRLFENELNRHQRADEMARQRRVQAANGRVIVVFSPKGG
                     VGCTLLACNLALALQSATQKRVALVDYSLQFGSVGALLNLQSAHTVAELASRQEEIDG
                     AILDDVTVTHPSGVRVLLRPNSLQEMEQITTEGLVAVLDGARKSFDYVVVDTWHVVEP
                     STLAVLEMADRLLLVTTPEVPALNAMRHFLDILKAYPHLRDKPQLLVNRQPSTGEVSL
                     KYVEEALGMKAIATVPSDGQMLTLAINEGTSLLQQKHANRPFVRNLNKLAESLAVQTD
                     LAGATTGTSAASRRFILSRRQNYS"
     gene            complement(3812..4642)
                     /gene="cpaB"
                     /locus_tag="VF276_00615"
     CDS             complement(3812..4642)
                     /gene="cpaB"
                     /locus_tag="VF276_00615"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEX6605393.1"
                     /translation="MKRPALLLLIVGLFAALAAGGLVYFVSSSAVAKTAPPPPPTPLP
                     TPTAIPMEVVVLAALDIPVRHLVTAADVVTRAYPLGVAPEDSFHSVTEVMSRTATTQV
                     FKGEMLLARQFIDAGGATGASTTIPPGKMLVAFPATDMLNATGAVHAGDHVDIMISIP
                     VSGTAALNSSAGATQEGAGTEAKTVVTQATMQNIEVYSTGTWTPTGPAADQNSAEVKI
                     ITFIVDHQEALILKYVKDSGGTIDLGVRSIADTQDVATDPVSLDYLVDLYHLIQVPSR
                     "
     gene            complement(4942..>6147)
                     /locus_tag="VF276_00620"
     CDS             complement(4942..>6147)
                     /locus_tag="VF276_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605394.1"
                     /translation="LEYHIMAGAPTCTNPPNTTGCYQVAPNDTARVPDWVDGVRVTAS
                     IQNPTTFARAVNINTVPVSAAAAAKIIGTCPVAAPPGPSLPFTRFRPDLENELRTSAN
                     DILHPYQFWSANSPSGDSSWKLLLSFRERSLYNPDPNSPPANPNPLQLLTGVDQRNGL
                     PSPGGNGVGPNYGSTGCTNSPLNPANCADMRGANPPDQALDLSNWTYWNWNGTIALTS
                     TWPLSAQVDTRDGDWLEAYNLQANLGNNVAAPIIDVINRDGVMTALSGPPYNLGKALD
                     KTVYVWGPDLATFQAGAQGGELGRTTEYAQQWNPGRINPTLQMGSWSNIAVVQKSNGQ
                     YGTSPTNATIGRVRFKFTYNFRFYAALQSNNSEVDGLFISEAQPGPVPPGNCGLTPGG
                     AIYTRLTDP"
BASE COUNT          990 a         1911 c         2128 g         1118 t
ORIGIN      
        1 ccaatgtaat caggattgat caggtagagc aggccgacca tgccgatggg cagcgccgtg
       61 agcagccagg ccgagagccg ctgctgggca ctcaagacct ggatctcgcg ctggatgcgg
      121 tggcgctcgc gcacggtggt ggcgatgaga tccaggatgt gggccaggtt gccgccggtc
      181 tgctgctgga tggtgatggc cgtgaccagg atctccatgt cctcggtggg cacccgttcg
      241 gccaggcggc gcaaggcctg gtccagcggc gcgcccaggc tgatcgcccg catcacccgc
      301 gtgtactcgg tcttgctcgg ttccggggct tcgcgggcga tgcgatccag cgcctggaac
      361 aggctgctgc cggatcgcac cgccccggcc aggatgttga ggatgtcggc cagttgggaa
      421 tcgaggcggc ggatgcgccg gctcacgctc cagtggagat aggccagggg cagccgccag
      481 ccgatgaaca gggcgggcag cagcgacagc caccacaccc ccggcgccag cagccagagc
      541 agcacggtca ggccaaaggc cgtggccgcg cggatcagcg cgaactcggt cagggtcaaa
      601 tgccactccg cgcgcgacag gtcgaggctc aggtccttgg ccttgacgcc cgacgcccag
      661 cgcgcgtcga gttccgcgaa cagcgcggcg cgttgatcct tgcgcacctg cgtttcctgc
      721 gcgggcgttg ccgcctcggc ggtcgccgca aactggcgca cgcgcgagta gactcgccgc
      781 tggggttgga ccacgagtag aacgatactc ccggtcagcc cgaagatcgc cagggccacc
      841 gccatccaaa cagctacata gattaacagc acgttgaacc tcgtcctaac gatcggccgt
      901 gcttagcgcc cattgcgcgc cggcaggccg ggggcgatcg gcaccacgaa gaagtcgggc
      961 gccaggaaga tccgtttctt ctccagtttg cgcatgacct gcgggcggat gcccgtcggg
     1021 atcaggtcgt agacttgctg gtcgccgcgc tgcacccact cgcggcggaa cacgtcctgc
     1081 atcaagatcg tgtcttgctc gaagccctgc acctcgctga cataagtcac tttgcgcgtg
     1141 ccgtcgtcca accgctccat gtagacgatc aggtcgaagg cggcggagat ctgttcgcgg
     1201 atggcgcgca ggggtagttc ggcgcgcgcc atcatcgaca tagtctcgat gcgcgagatg
     1261 gcatcgcgcg ggctgttcga gtggaccgtg gtcatacagc cgtcatggcc ggtgttcatg
     1321 gcctggagca tatccagcgc ttcggcgtcg cggcactcgc cgacgatgat gcggtcggga
     1381 cgcatgcgca ggctggtcac gaccaggtcg cggatagtca cggagttctg ccgttccatg
     1441 ctctgcgggc gcgactccag ccggatcacg tgcacctgtt gcaactgcag ttccgcggcg
     1501 tcctcgatgg tgatgatccg ctcctccgac gggatgaagg aggagagcac gttgagcagg
     1561 gtcgtcttgc cgctgctggt gccgccggcc accaggacgt tcaacccggc gcgcacggcg
     1621 gcctccagga agatcaccgc ttgctgcgag agggcgccca ggttcatcag gtcttccacg
     1681 gtgagcggca cgcgggcgaa cttgcggatg gtcaggatcg ggccggacag cgacagcggc
     1741 gggatgatgg cgtggacgcg cgagccgtcg ggcaggcggg cgtcgaccat cggcgaggac
     1801 tcgtccacgc ggcggccgag cggcgccacg atgcggctga tgatgcgcat gacgtgttcg
     1861 tcgtctttga agttgatgtc ggtcagcgtg atcttgcctt gccgctcgac ccacacctgg
     1921 tgggggccgt tgaccatgat ctcgttcacg gcgtcgctgt tgatcaactg ctggatcggc
     1981 ccatagccca tgatctcggc acagacaagc tcgaacagcc ggccacgctc gctgcgattg
     2041 agcggcacgt cttcctcggc catgaagcgg ttaaagagcc gttcgacctc ctgccgcaag
     2101 ccgaccgcat cgttgaggtc cacagagtcg cgtagttcgc cgataatttt gctgtgcagg
     2161 cgatgctgca gatcgacggc gttgggcctg ctcaacatgg cgctgatggt gcggctgctg
     2221 gtgggcgggc cgaggaccgt ggcgttcagt ggccccgtgc cggggcgggc gcccacgaca
     2281 gccggattca gcgggccgct gccgggacgg ccccccgccg cgccgttcgt ggaagcgggg
     2341 cgggcctccc cgctgccggc cagcggcgcg ccgctgaggg gccggtaggg gctgggcacg
     2401 gagaccgagt ccagcggcgt ggacgacggc gccgcggggg cggccggctc gccggggggg
     2461 gtcaggctgt tcgatttgct ggggatgata ggcatcgcaa tacgtctcct gctgtgctat
     2521 gcgcttacga atagttctgg cggcgggata agataaagcg ccgcgacgcc gcgctggtac
     2581 cggttgtcgc cccggccagg tcggtctgca cggccaggga ttccgcgagc ttgtttaagt
     2641 tgcgcacaaa gggccggttg gcgtgttttt gctgcaacag cgaggtgcct tcgttgatcg
     2701 ccagggtcaa catctgcccg tcgctgggca ccgtggcgat cgctttcatt cccagcgctt
     2761 cctcgacgta cttcaggctc acctcaccgg tgctcggctg gcgattgacc agcagttgcg
     2821 gcttgtcgcg caggtgcggg taggctttga ggatatccag gaagtggcgc atcgcattca
     2881 gcgccggcac ctccggcgtg gtgacgagca gcaaccggtc ggccatttcg agcacggcca
     2941 gggtggacgg ctcgaccacg tgccaggtgt ccacgaccac gtagtcgaag cttttccgcg
     3001 cgccatccag caccgcaacc agtccctcgg tcgtgatctg ttccatctcc tgcaagctgt
     3061 tgggccgcag cagcacgcgc accccggacg gatgggtcac ggtcacgtcg tcgaggatcg
     3121 ccccgtcgat ctcctcctgc cgggaggcca gttcggcaac ggtatgggcc gattgcaggt
     3181 tcaacagggc gcccacactg ccgaactgca ggctataatc caccagggcg acgcgcttct
     3241 gggtggcgct ctgcaaggcg agcgccagat tgcacgccag cagcgtgcaa cccacgccgc
     3301 ccttggggct gaagaccacg atcacgcgcc cgttggcggc ctggacccgg cgctggcgcg
     3361 ccatctcgtc ggcgcgctgg tggcggttca gctcgttctc gaacaggcgc tggatggtgc
     3421 cgaccacgtc gtgcgcggcc agcggcttgc gcaccaggcc gcgcgccccg gccaccatcg
     3481 cctgcccgat gtcgagcggg tcaccaaaat cggtcaccag gatcacctgg gacgcgggcg
     3541 ccacatcgag cacctggctg atcagcgcgg gcatgcctgc cgggtccaga ttcgtggaga
     3601 ggagaaagat gtcgggttcg agttcggcca gcgccagcac ttcggcgtgg gccctggccg
     3661 tgccggtgac gcggatcttc gggccttgca gcaacacggc gcgggtgcgg gcgagttcgt
     3721 cggcatcggc atcgcccacc acaacgttga tcgtatagtc gggtaactgg gtcatgaaac
     3781 gcgttcctcc ttgccgggca gcgccgggac gttagcgcga cggcacctgg atcaggtgat
     3841 agagatcgac cagatagtcc agcgaaaccg gatcggtcgc cacatcttgc gtgtcggcga
     3901 tggaccggac gccgaggtcg atggtgccgc cgctgtcttt gacgtatttc aggatcaggg
     3961 cttcctggtg gtccacgatg aaggtgataa tcttcacctc agcgctgttc tgatcggccg
     4021 cggggccggt cggggtccag gtgccggtgc tgtagacctc aatgttctgc atcgtggcct
     4081 gggtcaccac cgtcttcgcc tcggttcccg cgccttcctg ggtggcgccg gcgctgctgt
     4141 tcagcgcggc ggtcccgctg accgggatcg agatcataat atcgacatga tcgccggcat
     4201 ggacggcgcc cgtggcgttc agcatgtcgg tggccgggaa ggcaaccagc atcttgccgg
     4261 gcgggatcgt ggtcgaggcc ccggtggcgc ctccggcgtc gatgaactgg cgagcgagca
     4321 gcatctcgcc cttgaagacc tgggtggtcg cggtgcggct catcacctcg gtgacgctgt
     4381 ggaacgagtc ctccggcgcc acacccaacg ggtacgcccg cgtaaccaca tcggcagcgg
     4441 tcaccaagtg gcggacgggg atgtccaggg ccgccagcac caccacttcc atggggatcg
     4501 ccgtgggcgt cggcaacggg gtgggcggcg gcggcggcgc ggtcttcgcc acggcgctgc
     4561 tgctgacaaa gtagaccagc ccgccggccg ccagcgcggc gaacaacccg acaattaaca
     4621 ggagcaaggc aggacgcttc attcggtgca attcccttct tcgatcacgt cgtatggccc
     4681 ggtgcgcggg ccaccccgcg ccacatgctg cttatgggac aagatgaaac cgcaggtgtt
     4741 tacgcttgcc gaggacaagt ataaacagat tctgtatatc cgctgtgaat agcggggtgg
     4801 gggcctgtaa taattttacc cgttgatcct gggttatctg gtggaattta catataaatc
     4861 gcgcggatta ttgttacagc ttgttaacca ccgctggagg ggggcgatcc gccccgacct
     4921 ccagcagcat cgtttgtggg atcaggggtc ggtgaggcgc gtatagatcg cgccgcccgg
     4981 agtcaggccg cagttcccag gtggcaccgg ccccggctgt gcctcggaga taaacaggcc
     5041 atccacctcg ctgttgttgc tctgcaaggc cgcgtagaac cggaagttat acgtgaattt
     5101 gaagcggacc cggccgatgg tcgcgttcgt cgggctggtg ccgtactggc cgttgctctt
     5161 ttgcactacg gcgatgttgg accagctccc catttgcagc gtgggattga tgcgtccggg
     5221 gttccattgc tgggcatact cggttgttcg ccccagttcc ccaccctggg cgcctgcctg
     5281 gaaggtggca agatccggcc cccagacata gacggtcttg tcgagcgctt tgccgaggtt
     5341 gtacggcggc ccgctcagcg cggtcatcac gccgtcgcgg ttaatcacgt cgataatcgg
     5401 ggcggcgacg ttgttgccca ggttggcctg caggttgtag gcttccagcc agtcaccgtc
     5461 gcgggtatcc acctgcgccg ataagggcca ggtgctggta agcgcgatgg tgccgttcca
     5521 gttccagtag gtccagttgg agagatccag ggcctggtcg ggcggattgg ctccgcgcat
     5581 atcggcgcag ttggccgggt tcagcgggct gttggtgcag ccggtcgagc cgtaattagg
     5641 gcccacgccg ttcccgcccg gcgacggtag cccgttacgc tgatcgacgc cggtgagcag
     5701 ttgcagcggg ttgggattgg ccggcggcga gttgggatcc gggttataaa gactccgctc
     5761 acggaaggag agcaagagct tccaactgga atcgccgctg ggcgagttcg ccgaccagaa
     5821 ctggtaggga tgcaggatgt cgttcgccga ggtccggagt tcgttttcca gatcgggccg
     5881 gaaccgggtg aagggcaggc tggggccggg cggcgcggcc accgggcagg tgccgatgat
     5941 cttcgcggca gcggccgcgc tcacgggcac ggtgttgatg ttgaccgcgc gcgcaaacgt
     6001 cgtcggattc tggatactcg ccgtgacccg cacgccgtct acccagtcgg gcacgcgcgc
     6061 tgtgtcattt ggcgcaacct ggtagcagcc ggtcgtgttc ggcgggttcg tgcaggttgg
     6121 cgcgccggcc atgatgtgat actccaa
//
LOCUS       DASUYC010000041         2573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1322063, whole genome shotgun sequence.
ACCESSION   DASUYC010000041 DASUYC010000000
VERSION     DASUYC010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322063"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..836
                     /locus_tag="VF276_00625"
     CDS             168..836
                     /locus_tag="VF276_00625"
                     /inference="COORDINATES: protein motif:HMM:NF016726.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEX6605395.1"
                     /translation="MGIVRIAFTRAIQDWWYALINLAILNLIWFGFVVTVVAGPPATA
                     ALLVVARHAAVGQGAEFSTFFSALRQLFWRAWKMGLVTALGTVILVFDIYFYADRLGG
                     SGLLYTTGIVFLLYVLMIWIEIQLIAWPLLVDRPEMQVRHVLRNAAVVTLRMPGASFL
                     LFFLVAVLVIVSVYLAPLTALALGVVVALMVQHYLHLAAPVLANFPPRPGEGFPDAPP
                     VSAE"
     gene            complement(932..2527)
                     /locus_tag="VF276_00630"
     CDS             complement(932..2527)
                     /locus_tag="VF276_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX6605396.1"
                     /translation="MSKQHLWWQEGVIYQIYPRSFMDSNGDGIGDLPGITSRLDYLQW
                     LGVDAVWLSPIYPSPMADFGYDVADYTGIEPMFGTMADFDRLLAEVHGRGMKLVLDLV
                     PNHTSNEHAWFLESRASRDNPKRDWYIWRDAAPGGGPPNNWLSAFGGSAWQWDEATGQ
                     YYLHSFAVEQPDLNWRNPEVEAAVFDAIRFWLDKGVDGFRVDVIYYMVKDAEFRDNPP
                     NPDYQPGQDPARTQIPVYSYNRPEVHDIARRMRTLFDAYDDRVIIGEIYLSPDELMKY
                     YGANLDEVHLPFNFQLILLPWDATIIGRAVSAYEALLPELGWPNWVLGNHDQHRIATR
                     VGRAQARVAAMLLLTLRGTPTCYYGDELAMVDGEIPPDLVRDPQELGKPGLGLGRDPE
                     RTPMQWDASPHAGFTTGTPWLPVAADYQTYNVAAERDDPESMLSFVRALLALRRDTSA
                     LSVGNYAEMPSGSADLFAFTRGHAGHEILVILNFSHAPQRFQAQSGPASADVLLSTLP
                     GRSGNVDLANLEVGADEGLILRL"
BASE COUNT          393 a          812 c          871 g          497 t
ORIGIN      
        1 ggcctggcct tcttgcgcgg ccagtacggc acgttctata atgtagtgct ggcggggtcg
       61 atgttcaaca ctctgccgat ggtgttgttg ttcatcatct tctcgcgctt ctatgtccag
      121 ggggcaacct acagcggcgt gaagggctag aagcacacgg gagcggcatg ggcatcgtac
      181 gcattgcgtt tacacgcgct atccaggact ggtggtacgc gctcatcaat ctagccatcc
      241 tgaacctgat ctggttcggc ttcgtcgtga cagtggtcgc cggtccgccg gcgaccgctg
      301 cgctactggt ggtggcgcgg cacgccgccg tggggcaagg cgccgagttc agcaccttct
      361 tcagcgcgtt gcggcaactg ttctggcgcg cctggaagat ggggttggtc accgcgctgg
      421 gcacggtgat cctggtcttc gacatctatt tctacgccga tcgcctgggc ggctccggct
      481 tgctctacac gacgggcatc gtcttcctgc tctacgtgct gatgatctgg atcgagatcc
      541 agctcatcgc ctggccgctg ctggtggatc gcccggagat gcaagtgcgc catgtgctgc
      601 gcaacgccgc cgtcgtgact ctgcggatgc cgggggccag cttcctgttg ttcttcctgg
      661 tcgccgtcct ggtgatcgtg tcggtgtacc tggcgcccct cacggcgctt gctctcggcg
      721 tggtggtggc gctcatggtg cagcattacc tccacctggc cgcgcccgtc ctggcgaact
      781 tccccccgcg ccccggcgag ggctttcccg acgcgccccc tgtctccgcc gagtaaccgc
      841 acgctaccgg tagagccgaa aagcttgaca taacgagggc gaacctgccg gcaggttcgc
      901 cccgtttgca ttggtccggc ccgtgccgaa cctacagacg caggatcagc ccttcgtcgg
      961 ctcctacttc gaggttcgcc aggtccacgt tcccgctgcg accgggcagg gtggacagca
     1021 ggacatcggc tgatgccggg cccgattgcg cctggaaccg ctggggcgcg tggctgaagt
     1081 tgagaatcac caggatctcg tgcccagcgt gcccgcgggt gaaggcgaag agatcggcgg
     1141 agccggacgg catctcggca tagttgccca cactgagggc ggacgtgtcc cggcgcaagg
     1201 cgaggagggc gcgcacaaag ctgagcatcg actccgggtc gtcgcgctcc gccgccacgt
     1261 tgtaggtctg atagtctgcg gcaacgggca gccagggcgt gcccgtggtg aacccggcgt
     1321 gcggcgaggc gtcccactgc atgggtgtgc gctccgggtc gcggcccagg cccagccccg
     1381 gcttgcccag ttcttgcggg tcgcgcacca ggtcgggcgg gatctcgccg tccaccatcg
     1441 ccagttcgtc gccataatag caggtgggcg tgccgcgcag ggtcagcagc agcatcgccg
     1501 ccacccgcgc ctgggcgcgc cccacccgcg tggcgatgcg gtgctggtcg tggttgccca
     1561 gcacccagtt gggccagccc agttccggca acagcgcctc gtaggcgctg acggcgcggc
     1621 caatgatcgt ggcgtcccag ggcagcaaga tcaactggaa gttgaagggc aggtggacct
     1681 cgtccaggtt tgccccgtaa tatttcatca attcatcggg ggacagatag atctcgccga
     1741 tgatcacccg gtcgtcgtag gcgtcgaata gcgtccgcat gcggcgggcg atgtcgtgca
     1801 cttcgggccg gttgtagctg tagacgggga tttgcgtccg cgccgggtcc tggccgggct
     1861 ggtagtcggg gttgggcggg ttgtcgcgga actcggcgtc cttgaccatg tagtagatga
     1921 catcgacgcg gaagccgtcc acgcccttgt cgagccagaa gcggatggcg tcgaacacgg
     1981 cggcttcgac ttcggggttg cgccagttca gatcgggctg ctccaccgca aacgagtgca
     2041 ggtaatactg cccggtcgcc tcgtcccact gccaggcgct gccgccgaaa gcgctgagcc
     2101 agttattcgg cggcccgccg cccggtgccg cgtcgcgcca gatgtaccag tcgcgcttcg
     2161 ggttatcgcg gctcgcgcgg gactccagga accaagcgtg ttcgttggag gtgtggttgg
     2221 gcaccaggtc caggaccagc ttcatcccgc gcccgtggac ctcggcgagc aagcggtcga
     2281 agtcggccat ggtgccgaac atcggctcga tgccggtgta gtcggccacg tcgtagccga
     2341 agtcggccat gggcgagggg tagatgggcg acagccagac ggcatcgacg cccaaccatt
     2401 gcagataatc gagccgggac gtgatgccgg gcagatcgcc gatgccgtcg ccgttgctgt
     2461 ccatgaagct gcgcgggtag atctggtaga tcactccttc ttgccaccac agatgttgct
     2521 tggacatatt gctccttatc ttcggtaaac gaacctgggc cggtggatgc ggg
//
LOCUS       DASUYC010000042         2351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1554751, whole genome shotgun sequence.
ACCESSION   DASUYC010000042 DASUYC010000000
VERSION     DASUYC010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554751"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VF276_00635"
     CDS             <1..267
                     /locus_tag="VF276_00635"
                     /inference="COORDINATES: protein motif:HMM:NF044708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605397.1"
                     /translation="DARRNGYPPGQQRAFVLARVLAENPIIIVGAEQPEVIRQAHLRT
                     APTMESALADAIALLGPRPADAPPLEVLIVPHALLTLPVVAAEA"
     gene            complement(488..841)
                     /locus_tag="VF276_00640"
     CDS             complement(488..841)
                     /locus_tag="VF276_00640"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX6605398.1"
                     /translation="MGTGMRRGWRRMVAGVLLAAATIGVVAGCGDTPTVGPTATAVPP
                     ELQAGQRVFLQYCNTCHPGGHQGVGKNLVNAGLTADKITTTVRNGKRNMPAFGTAQIS
                     DSDLQSLIGYVQALR"
     gene            complement(939..1024)
                     /locus_tag="VF276_00645"
     tRNA            complement(939..1024)
                     /locus_tag="VF276_00645"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(988..990),aa:Ser,seq:tga)
     gene            complement(1046..2299)
                     /locus_tag="VF276_00650"
     CDS             complement(1046..2299)
                     /locus_tag="VF276_00650"
                     /inference="COORDINATES: protein motif:HMM:NF013473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6605399.1"
                     /translation="MNQAIFGQWGRRLGPTARGSLFYLGVSGGSAAFLPFVNVFYADR
                     GLSGREIGLLAAAGPVVALLAAPLLTALADRHGWQRQVLVLGLAGVAVTTLLIPLAPA
                     FVVLLAVVAGNGLIGSTVGSISDGMIAQMATRHRINYGKMRLWGSAAWVVMSLLGGFL
                     WPRFGLWLMFPLASLLFAATMVVARLLGEDGSSAKVERRPFRLTSGAARLWIVLICAV
                     LLSLGMVMARTFSAIYIDRVSGQMLVGLYAAVAAAIEIPIMLWTERVLRRVGGPFTLA
                     LAGLLLGGAFIGLATIPRPELLLLAALLEGIGYALFVTAIVQLVAGWAPHGQVATYQG
                     LLNAGHYGLAPLLAGLMGGALFDATGPQAVFVAGAGAVAVGMAVLLVTQVLGVFSGDP
                     AHDIPVPPSGETGADAPAGRLDPPG"
BASE COUNT          441 a          838 c          722 g          350 t
ORIGIN      
        1 gacgcgcggc gcaacggcta tccgcccggc cagcagcgtg cctttgtgct ggcccgcgtg
       61 ctggccgaaa acccgatcat catcgtcggc gcagagcagc cggaggtgat ccgccaggcc
      121 cacctgcgca ccgcgcccac catggagtcc gccctggccg atgccatcgc gctgctggga
      181 ccgcggcccg ccgatgctcc gccgctggag gtgctgatcg tgccccacgc gctgctcacg
      241 ctcccggtgg tcgcggcgga ggcctagctc tttccctcgt tgtcatgctg aacgcagtgc
      301 agcatctcgt ctcgttggga taagcgacga gactcttcgc tgtgctcaga gtgacaagcc
      361 tgcattgtca tgctgaacgc agtgaagcat ctcgtctcgt tggggtaagc gccgagactc
      421 tttgctgcgc tcagagtgac aagcctttag acgagactct gagcgcagcg aagagtgaca
      481 ggggggctta gcgcagggcc tggacatagc cgatcagcga ttgcaggtcg ctgtccgaga
      541 tctgcgccgt gccgaaggcg ggcatgttgc gcttgccgtt gcgcaccgta gtggtgatct
      601 tgtctgcggt cagcccggcg ttgaccaggt tcttgcccac gccctggtgg ccgccggggt
      661 ggcaggtatt gcaatactgc aaaaagaccc gctgccccgc ctgcaactcc ggcggcacgg
      721 cagtggccgt cggccccacc gtcggggtat cgccgcagcc ggccacgacg ccgatagtgg
      781 ccgccgccag cagcaccccg gccaccatgc gccgccaccc gcggcgcatt ccagtaccca
      841 tcgctcgttc ctgtcccatc gctatgcgtg cctcacacaa atgatggtca gcattcagcc
      901 aaagctaaac actgaccact gaccactgac cactcgtttg gcggagaggc agggattcga
      961 accctgggga ctcacgtcca gttgttttca agacaaccgc aataggccac tctgccacct
     1021 ctccctgcgc cctattctac ctgctttagc ccggagggtc aagccgcccg gcgggcgcat
     1081 cagcgccggt ttcaccgctg ggcgggaccg ggatgtcatg ggccggatcg ccggaaaaca
     1141 cgcccagcac ctgggtcacc agcaataccg ccatcccaac cgccacggcc ccggcgccgg
     1201 cgacgaagac ggcttgcggt ccggttgcgt cgaaaagggc accccccatc agtccggcca
     1261 gcaacggcgc gaggccgtag tgaccggcat tgagcaggcc ctggtaggtc gctacctgtc
     1321 cgtggggcgc ccacccggcg actagctgca cgatcgccgt cacgaacagg gcatagccga
     1381 tgccttccag cagcgccgcc agcagcagca actccgggcg gggaatcgtt gccaatccaa
     1441 taaaggcgcc gcccaggagg agaccggcca gcgccaaagt gaacggcccg cccacccgcc
     1501 gcagcacccg ctcggtccac aacatgatcg ggatctcgat ggcggcggcg acggccgcgt
     1561 acaggccgac cagcatctgc ccgctcacgc ggtcgatgta gatggcggag aaggtccgcg
     1621 ccatgaccat gcccaggctc agcaacaccg cgcagatcag cacaatccat aagcgcgctg
     1681 cgccggaggt caggcgaaac ggccggcgtt ccactttggc gcttgacccg tcctcgccga
     1741 gcaggcgcgc gacgaccatg gtcgccgcaa acagcaggct cgccagggga aacatcagcc
     1801 acaggccaaa ccgcggccag aggaagccgc ccagtaagga catcaccacc caggccgccg
     1861 atccccatag gcgcatcttg ccgtagttga tccggtgccg ggtcgccatc tgcgcgatca
     1921 tgccgtcgga gatggagcca accgtgctac caatcaggcc gttgccggcc accaccgcca
     1981 gcagcaccac gaacgccggc gccagcggta tgagcagtgt cgtcaccgcg acgccggcca
     2041 gacccagcac cagcacctgc cgctgccagc cgtgccggtc cgccagcgcc gtcagcagcg
     2101 gcgcggcgag cagcgccact accggcccgg ctgcggccag caggccgatt tcgcggcccg
     2161 agagcccacg atcggcatag aacacgttga cgaaaggtaa gaacgcggca cttccgccgg
     2221 agacgcccag gtaaaagagg ctaccccgcg ctgtcgggcc cagtcgcctg ccccattgcc
     2281 caaatatcgc ctggttcaca tgtatctcac tggtaatctg ccgagcatac aaaagagcgc
     2341 cgggtcatgc c
//
LOCUS       DASUYC010000043         2061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_840428,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000043 DASUYC010000000
VERSION     DASUYC010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840428"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1092
                     /locus_tag="VF276_00655"
     CDS             <1..1092
                     /locus_tag="VF276_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HEX6605400.1"
                     /translation="DPARSWLAYVAGVLVVLHRALGLRLLGGLRLLIDSDVPIGKGVS
                     SSAALEVATMQAVCAAAGWTFDGRELALLCQRVENLVVGAPCGVMDQMAAACGEQDRL
                     LALLCQPAELQPSVALPPDVEVWGIDSGIHHAVTGADYGSVRVGAFMGYRIIAALAGL
                     PATLREPGRVAVEDPQWRGYLANITPSVWESTYRTQVPEMMDGATFLAHYGGSTDSVT
                     RIDPERTYPVRAATAHPIWEHHRVRLFRALLRKRRLAEEDRTLLGELMYQSHASYSAC
                     GLGAEGTDRLVALVREAGPAAGLYGAKITGGGSGGTVAVLARRGAGTAVEALAARYTQ
                     DTGHPAAVLGGSSPGAMQFGFIRLRVTPG"
     gene            complement(1126..>2061)
                     /locus_tag="VF276_00660"
     CDS             complement(1126..>2061)
                     /locus_tag="VF276_00660"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEX6605401.1"
                     /translation="FIAVHPNRRWLYAVSETSMDQEGVPGAVWAMACTRAPWGFSAIN
                     QQASGGDWPCHLAIHSAGRWLLVSNYASGTVGVLPIGDDGALGELSDLARHAGSGPHP
                     VRQQGPHAHSATFTPDERFVIVADLGLDALMLYAFDAAAGRLQPHTQVQTRAGAGPRH
                     MAFHPNGTRLYVANELDNTVAVYDYDASQGGLRAGQILDTLPPGAPENTVADIHITPA
                     GDRLYVSNRGYDSVAVFDVAADGQLAPVAIRPCGGQWPRNFAIAPGGRFLLVANQYSD
                     TISTLPVLDTADGLDAPSVQTPVPGASCVQFIAAA"
BASE COUNT          284 a          701 c          762 g          314 t
ORIGIN      
        1 gacccggcgc gtagctggct ggcctacgtc gccggggtgc tggtggtgct gcaccgggcg
       61 ctggggctgc ggctgctggg cggcctgcgg ctgttgatcg actccgacgt gcccatcggc
      121 aagggcgtga gttcgtccgc cgcgctggaa gtggcgacga tgcaggcggt gtgcgccgcc
      181 gcaggctgga cgttcgatgg gcgcgaactg gcgctgctct gccagcgggt ggagaacctc
      241 gtcgtgggcg cgccctgcgg ggtgatggac cagatggccg cggcctgcgg ggaacaggac
      301 cgcctgctgg cgctgctctg ccagcccgcc gaattgcagc cctcggtcgc cttgccgccc
      361 gatgtcgagg tctggggcat cgactccggc atccaccacg cggtcacggg cgccgattac
      421 ggctcggtgc gggtgggcgc gttcatgggc tatcgcatca ttgccgccct ggccggccta
      481 cccgctacgc tgcgggagcc ggggcgggtc gccgtggagg acccgcaatg gcggggctat
      541 ctcgctaaca tcacgccctc ggtctgggaa agcacctacc ggacgcaggt cccagaaatg
      601 atggatggag cgaccttcct ggcccactat ggcggatcga ccgacagcgt cacccgcatc
      661 gatcctgaac gaacctaccc ggtgcgggcg gccacggcgc acccgatctg ggagcaccac
      721 cgcgtgcgcc tgttccgcgc cctgctgagg aagcgccgct tggccgagga agaccggacg
      781 ctgctgggcg aattgatgta ccagtcgcac gccagttaca gcgcctgcgg cctgggcgcc
      841 gaggggaccg accgattggt ggccctggtg cgcgaagccg gaccggcggc ggggttgtat
      901 ggcgccaaga tcaccggggg cggcagcggc ggcacggtcg cggtgctggc gcggcgcggc
      961 gcgggcacgg cggtcgaggc cctggctgcg cgttacacgc aggataccgg acacccagcc
     1021 gccgtcctgg gcgggtcgtc accgggcgct atgcagttcg gattcatccg cttgcgcgtt
     1081 acccctggat aacgtcccca tcagtccgtc acgccatgca gcgcttcaag cggcggcgat
     1141 gaattgcacg cacgatgcgc ccggcaccgg ggtctgcacg ctgggggcat ccaggccgtc
     1201 cgccgtatcc agcaccggca gcgtcgagat ggtgtcgctg tactggttcg ccaccagcag
     1261 gaaccggccg ccgggcgcga tcgcgaagtt gcgcggccat tgcccgccgc agggccggat
     1321 ggcgacaggc gccaactgcc catcggcggc cacgtcgaag accgccacgc tgtcgtagcc
     1381 gcggttggag acgtagagcc ggtcgcccgc gggggtgatg tggatgtcgg ccacggtgtt
     1441 ctccggtgcg ccgggcggca gcgtatcgag gatctgcccg gcccgtaacc cgccctggct
     1501 cgcgtcgtag tcgtacacgg ccacggtgtt gtccagttca ttggcaacgt acagccgcgt
     1561 gccgttcggg tggaaggcca tgtgtcgcgg cccggccccc gctcgcgttt ggacctgggt
     1621 atgcggctgc aaccggcccg ccgcggcgtc gaacgcatat agcatcagcg cgtccaaccc
     1681 caggtcggcc acgatgacga agcgctcgtc gggtgtgaag gtcgccgagt gggcgtgcgg
     1741 cccctgctgg cgcacggggt ggggcccgct gccggcgtga cgcgccaggt cgctcagttc
     1801 gcccagcgcg ccgtcgtccc cgatgggcag cacgccgacg gtgccgctgg cgtagttgga
     1861 caccagcagc cagcgcccgg ccgaatggat cgccaggtga cacggccagt cgccgccgct
     1921 ggcctgctga ttgatcgcgc tgaagcccca cggcgcgcgg gtgcaggcca tcgcccagac
     1981 cgcgccgggc acgccctcct ggtccatgct ggtctcgctg acggcgtaga gccaccgccg
     2041 gttggggtgg acggcgataa a
//
LOCUS       DASUYC010000044         3188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1919599, whole genome shotgun sequence.
ACCESSION   DASUYC010000044 DASUYC010000000
VERSION     DASUYC010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1919599"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VF276_00665"
     CDS             complement(<1..379)
                     /locus_tag="VF276_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX6605402.1"
                     /translation="MADLMMPRVEVVATAPDYRRFAIGPLEKGYGLILGNALRRILLS
                     SLPGAAVTSVRIEGVYHEFSTLPHVKEDVTEIVLNVKKLRLRSFSDRPVRLQLSATGP
                     GKVRATDIQLPSTVELINADQVLA"
     gene            complement(497..1126)
                     /gene="rpsD"
                     /locus_tag="VF276_00670"
     CDS             complement(497..1126)
                     /gene="rpsD"
                     /locus_tag="VF276_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX6605403.1"
                     /translation="MARYTGPVCKLCRREGLKLFLKGDRCFTPKCAIERRNQPPGQHG
                     QRRRGKVSDYGLQLREKQKARRTYGIMERQFKRHFEMAEGRPGVTGENLLQVLETRLD
                     NVVYRLGFANSRPQARQLVSHGHFLVNGVKTDIPSYICKATDVIAVRPEHATDEYFKV
                     VAAEIGRKDIPTWLTLDPRNLSGTVISLPARADLDVNLNEQLIVEFYSR"
     gene            complement(1234..1656)
                     /gene="rpsK"
                     /locus_tag="VF276_00675"
     CDS             complement(1234..1656)
                     /gene="rpsK"
                     /locus_tag="VF276_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX6605404.1"
                     /translation="MAEKKQQAGAPRSARAARGRRRERKMVPRGQAHVQSSFNNTIVT
                     LTDPTGNVLSWGSAGASGFKGSRKSTPYAAQITADGAAKRAMEHGLRQVDVFVKGPGS
                     GREAAIRALQGAGLQVTSITDVTPIPHNGCRPPKRRRV"
     gene            complement(1702..2079)
                     /gene="rpsM"
                     /locus_tag="VF276_00680"
     CDS             complement(1702..2079)
                     /gene="rpsM"
                     /locus_tag="VF276_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016484177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX6605405.1"
                     /translation="MARIAGVDIPRDKQVATALTYIYGIGPTSARKIIARTGIAPETR
                     VRMLSEEEVNRLREIIDREYRVEGDLRREVNLNIKRLIEIKCYRGIRHQRGLPVHGQR
                     TRTNGRTRRGPKRVVAAKKKARK"
     gene            complement(2116..2229)
                     /gene="rpmJ"
                     /locus_tag="VF276_00685"
     CDS             complement(2116..2229)
                     /gene="rpmJ"
                     /locus_tag="VF276_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012626782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEX6605406.1"
                     /translation="MKVKASVKTRCERCRVIKRKGVIMVICTNPRHKQRQG"
     gene            complement(2251..2478)
                     /gene="infA"
                     /locus_tag="VF276_00690"
     CDS             complement(2251..2478)
                     /gene="infA"
                     /locus_tag="VF276_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX6605407.1"
                     /translation="MPGRQGARELEGRVIAPQPNAMFTVELENGNRVLAHVASEVRVT
                     IGRILPGDRVKLELSPYDISRGRITRRCHNP"
     gene            complement(2767..>3188)
                     /locus_tag="VF276_00695"
     CDS             complement(2767..>3188)
                     /locus_tag="VF276_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX6605408.1"
                     /translation="VGVGKIDPAAQKMIDVGWESLWAGIKLARAGNRLEDISATIQEV
                     LERNGYGVVRDLVGHGIGRRLHEPPNVPNFGRRGQGLRLVPGLVLAIEPMLTQGSYEG
                     RTLDDDWTIMTVDQGLAVHVEHTVAITDGDPEILTLP"
BASE COUNT          526 a         1042 c          990 g          630 t
ORIGIN      
        1 tcgccagcac ctggtcggca ttgataagct cgacggtact cggcaactgg atatcggtcg
       61 cgcggacctt gcccggtccg gtggccgaaa gttgcagccg caccgggcgg tcgctgaacg
      121 agcgcaggcg cagctttttc acattcaaga cgatctcggt cacgtcctct ttcacatgag
      181 gcagtgtcga gaactcgtgg tagacgcctt cgatgcgcac gctggtcaca gcggcgcccg
      241 gcagcgacga aagcaggatc cggcgcaagg cgttgcccag gatcagcccg taacccttct
      301 ccagcggccc gatggcaaag cgccgatagt caggagcggt ggctactact tccacccggg
      361 gcatcatcag atcagccaca cgaacccctc ctctagcaat atcatgatca agatccagca
      421 cggcggtaac atcctatctg gcccgcggtt gcccgcgagc gcaagggccg gggcgtccgg
      481 cccgttgctc tccgtattac cggctgtaga actcgacgat gagctgctcg ttgaggttca
      541 cgtccaggtc ggcccgtgcc ggtaaactaa tcacggtgcc gctcaggttg cgcggatcca
      601 gcgtcagcca ggtcgggatg tccttgcgcc cgatttccgc ggccacgacc ttgaagtact
      661 cgtccgtggc gtgctcgggg cgcacggcga tcacatcagt cgctttgcag atgtaggacg
      721 ggatatcggt tttgacgccg ttgaccagga aatgcccgtg ggagacaagc tggcgcgcct
      781 gggggcgcga attggcgaag ccgaggcggt agaccacatt gtcgaggcgg gtctccagca
      841 cctgcagcag gttctcaccg gtcacgccgg ggcggccttc ggccatctcg aaatggcgct
      901 tgaactgccg ctccataatg ccgtatgtgc gccgcgcctt ctgcttctcg cgcagctgca
      961 ggccgtagtc agatactttg ccgcgccgcc gctgcccatg ctgtccgggg ggctgattac
     1021 gccgctcaat cgcgcacttg ggcgtgaagc agcgatcccc cttgaggaac agcttgagtc
     1081 cttcgcgacg gcacagttta caaaccggtc cggtataacg ggccatggtt tttcctcctg
     1141 tgtcgtatgt tctagattgt catgttgctc ggcgggtgag gccggaggcc agaatagtcg
     1201 atcagcccgc gagcagattg ttatgtcaat aaatcagacg cggcggcgct taggcgggcg
     1261 gcagccgttg tgcgggatgg gcgtcacgtc ggtgatcgag gtgacctgca acccggcacc
     1321 ctgcaaggcg cgaatcgcgg cttcgcggcc cgaccccggc cccttgacga acacatccac
     1381 ctgccgcagt ccatgctcca tcgcgcgctt ggcggcgccg tcggcggtga tctgggccgc
     1441 atacggggtg ctcttgcgcg agcctttgaa gcccgacgcg ccggcactac cccaactcag
     1501 cacattgccg gtcgggtcgg tcagcgtgac gatggtattg ttgaagctgg actggacatg
     1561 cgcctggccg cgcggcacca tcttgcgctc gcggcggcga ccccgtgcgg cacgcgcgga
     1621 gcgcggcgcc ccggcctgct gctttttctc agccatctta tctcctctaa acgggacctg
     1681 gcctagcgga ccaggtcctg atcacttccg ggccttcttc ttcgccgcga cgacccgctt
     1741 ggggccacgg cgcgtacgac cattggtgcg ggtgcgctgc ccatgcaccg gcagcccgcg
     1801 ctgatggcgg atgccccggt agcacttgat ttcgatcagg cgcttgatgt tcaggttgac
     1861 ttcccggcgc aggtcgcctt cgacgcggta ctcgcggtcg atgatctcgc gcaggcggtt
     1921 cacttcttct tcgctcagca tccgcacccg cgtctcgggg gcgatcccgg tccgcgcaat
     1981 gatcttgcgc gccgaggtcg ggccgatgcc gtaaatatag gtcagggcag ttgcaacctg
     2041 cttgtcgcgc gggatgtcta ccccggcaat tcgtgccacg ctcagttcct cctttccgtc
     2101 agattgggcg cgggcttagc cctggcgctg cttgtgccgc gggttggtgc aaatcaccat
     2161 gatcacgcct ttgcgcttga tcacgcggca ccgctcgcag cgggtcttca ctgatgcttt
     2221 aaccttcatc ttggcccaca ctcctctggt ttacggatta tgacagcggc gggtgatccg
     2281 cccccgcgat atatcatagg gcgacagctc gagtttcacc ctgtcgcccg gcaaaatccg
     2341 cccgatagtg acgcggactt cgctcgcaac gtgggccagg acccggttgc cgttctccaa
     2401 ctcgacggtg aacatcgcat tgggctgggg tgcgatgacc cggccctcga gttcccgtgc
     2461 gccctgccgt cccggcatat gctgcgtcct ctttacccgg cccaaacgcc ggatcaccta
     2521 cagacaatat gcttcctgcg ccacaggggg caccaagcgt caattatacc ataacgccct
     2581 gctatttgtc cagtcgggca caagccgtca gctatcagcc gtcagccgtc aggagacgat
     2641 agtatccgca ggtgcggtct acgctgcgac aaacaagacg agactcttcg ctgctctcag
     2701 agtgacaagc ctgggaatcc ctgacggctg acggctgata gctgacggct gatagctgac
     2761 ggctcgctac ggcagcgtga gaatttcggg gtcgccgtcg gtgatggcga ccgtgtgctc
     2821 gacgtggacg gccaggccct ggtccacggt catgatggtc cagtcgtcgt ccagggtgcg
     2881 gccttcgtag ctgccctggg tgagcatcgg ctcgatggcg aggaccaagc cgggcaccag
     2941 gcgcaggccc tggccgcgcc ggccaaagtt gggcacattg ggcggctcgt gcaggcggcg
     3001 gccaatgccg tggcccacga ggtcgcgcac cacgccgtag ccgttgcgct ccagcacttc
     3061 ctggatcgtc gcgctgatgt cttccagccg gttacccgcc cgtgccagct tgatgcccgc
     3121 ccagagcgac tcccaaccca catcgatcat cttctgcgcg gcgggatcga tcttgcccac
     3181 gccgactg
//
LOCUS       DASUYC010000045         5020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1707117, whole genome shotgun sequence.
ACCESSION   DASUYC010000045 DASUYC010000000
VERSION     DASUYC010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5020
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1707117"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..1249
                     /locus_tag="VF276_00700"
     CDS             221..1249
                     /locus_tag="VF276_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX6605409.1"
                     /translation="MTVSTTESNARAAQPAADTLTPEQFRVAATAIEEEVGKVIVGQR
                     ALVRQVLITLVAGGHALLEGVPGLGKTALVRALAEALDCEFGRIQFTPDLMPADIIGT
                     NILVEDEQGRRQFQFQRGPIFANLVLADEINRATPKTQSALLEVMQEGTVSVAGTVYR
                     LQAPFIVLATQNPLEMEGTYPLPEAQLDRFLFKLNVVFPTAAELLTIVERTTGTPPAA
                     LRPVVDSDDIVQMQQLARQTPIAEHVAAYGVRLLRGTHPDAPESTPLVRKYVRYGASP
                     RGLQAMILAGKIMALLDGRFNVAFKDIRAAALPALRHRIILNFDGEAEGIAPDALITS
                     ILDATPTE"
     gene            1388..3373
                     /locus_tag="VF276_00705"
     CDS             1388..3373
                     /locus_tag="VF276_00705"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA and WFA domain-containing protein"
                     /protein_id="HEX6605410.1"
                     /translation="MGLLAPLALAFVPVLGLIIALYLLRLRRPETPVSSLLLWRALLR
                     DQEANAPWQRLSTSLLLLLQLAIAAVLILALARPWSVAATPGGSNLVIVLDTSASMGA
                     TDGPAGRTRLAEAVRDAGARISALPANGTAALIAAGTHARIVSPATGDRDALRRALDT
                     LQPAPTTTDLSEALSLAAGVAAASADPEVLVYSDGQFPDPRGTVPALAAPLHFVAMGT
                     RGANQAIVALSVRRTAATLSLLAQVANADTAQVTRRLDIALDGVAWAGRTLTLPPGET
                     TQVLFDEVPPAAQVVQAHLAGADDLAVDDTAWTINHAAEPAPVLLVTDGNRFLQNALV
                     LLPNVALDHTAPADYSPALTATLTVFDRTVPSTTLPTGNLLFVAPPTSTTIFEVTGVL
                     TTPNPLPQVAPASVDPGTAPADGAGGDPLLRYTDLSDLHIARAAHIARPPWARVVLDS
                     DAGPLILAGEVEGHRVVIIGFDLHDSDLPLQMSYPLLMRNLVGYLLPEAAGDLAASVQ
                     PGDPVPLAAAVQEGVDRLTVRGPTGAQLASYAVGPDQFRFTFTGADTPGVYIVSQWVG
                     DREVRREGFTANLFSLDEARTAPRADPPLPAGRPVGAADVSDVQTGQGQFEWWQGLAA
                     LALLALIAEWLITHRLGLRQLGRRLRGRPLEDPLGHS"
     gene            3360..3773
                     /locus_tag="VF276_00710"
     CDS             3360..3773
                     /locus_tag="VF276_00710"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6605411.1"
                     /translation="MDIREFAWDDYDAVYALWERLHMLKPSIDSRVALAGCLERNPGL
                     FLVAVAPDGHPAGTVLGTFDGRRGYLYHVAVDPAYRRQGIGRALMTEIMDRLRARGAV
                     KITLRVARENQSAIAFYRSVGLDADEHVLGMSIEY"
     gene            3864..>5020
                     /locus_tag="VF276_00715"
     CDS             3864..>5020
                     /locus_tag="VF276_00715"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX6605412.1"
                     /translation="MDLSWAGLPLLFTDPWWLVAFALLPPLIVLGLWEGRTVRRRRVA
                     LGVVRAGPERWRSRVSAIVRATILSCVLLALAGLQLVRSAGPVSTVYLVDVSDSVSAN
                     DRAAAHAYVQRALDAARSDERSAVVLFAGQAQVVRPFAATGPLAAFDAQLPAGGTDIA
                     AALRLGTALLPADGPRRLVLISDGQETDEQARTEATTAAAAGIPVSVVPLHSGALNEV
                     AVDAVGAPSMVPSGQGFEIQVRLTSTAAEGAHLRLFQDDTLLAERDVSLQKGSNLFTF
                     PATARDEGFHSYRAVVSAVDDRWAQNNEAAAVTVVQSPPRVLIVAGQPDDAEPLRVAL
                     EAAHIEPQVAKAADVPHTVDGLAAYDSIVLANTPAGALGDATLAAMQAYVR"
BASE COUNT          715 a         1849 c         1749 g          707 t
ORIGIN      
        1 cgtggtgcgc agcgggccgg gcaccaatcc cggcgtgaaa accccgtata acaaagtcat
       61 cggcaagtac caggacaccg ccgcgcaggc cctcgaccgc acctatattc ccgccgacgc
      121 caagcagtac gttcgcgact attttaactc gcttgcccca acaccctgac cccggttgct
      181 accctcgccg ccgggtcatc atgatcgaac aggagccttt atgaccgtca gcacgaccga
      241 gagcaacgcc cgcgccgcgc aaccggcggc ggataccctg acgccggagc agttccgcgt
      301 ggcggccacg gccatcgagg aagaagtcgg caaagtaatc gtgggccagc gcgccctggt
      361 gcgccaggtc ttgatcaccc tggtggccgg gggccacgcc ctgttggaag gcgtgcccgg
      421 cctgggcaaa accgccctgg tccgcgcgct ggccgaggcg ctcgactgcg agttcgggcg
      481 catccagttc acccccgacc tgatgcccgc cgatatcatc ggcaccaaca tcctggtcga
      541 ggacgagcag ggccgccggc agttccagtt ccagcgcggc ccgatcttcg ccaacctggt
      601 gctggccgac gagatcaacc gcgccacgcc caagacccaa tcggccttgc tcgaagtgat
      661 gcaagagggc actgtctccg tcgcgggcac cgtctaccgc ctgcaagccc cgttcatcgt
      721 gctggcgacg cagaacccgc tcgaaatgga gggcacctat ccgctgcccg aggcccagct
      781 agaccgcttc ctgttcaagc tcaacgtcgt cttccccacc gccgccgaat tgctgaccat
      841 cgtggagcgg accaccggca cgccgcccgc ggccttgcgc cccgtggtcg acagcgacga
      901 tattgtccag atgcagcaac tggcccgcca gacgcccatc gccgagcatg tcgcggccta
      961 tggcgtgcgc ctgctgcgcg gcacgcatcc cgacgcgccg gagtcgaccc cgctggtccg
     1021 caagtatgtg cgctacgggg ccagcccgcg tggcctgcag gcgatgatcc tggccggcaa
     1081 gatcatggcc ctgctcgacg ggcgctttaa cgtggccttc aaagacatcc gcgcggcggc
     1141 cctgcccgcc ctgcgccatc gcatcatcct gaacttcgac ggcgaggccg aagggatcgc
     1201 ccccgatgcg ctgatcacgt cgattctgga cgcgacgccg acggagtagc tatcagccgt
     1261 cagccgtcag ccgtcagcta tcaggaatcg gctaaagaag ccgtccacac ctctccgccg
     1321 gcaatcgccg gcagatgtta cgtctcctga cggctgacgg ctgacggctg accactgacc
     1381 actgacgatg ggattactcg caccgctggc cctggccttc gtgcccgtgc tgggcctgat
     1441 catcgccctc tacctgctgc ggctgcggcg gccggagact ccggtgtcga gcctgctgct
     1501 ctggcgcgcc ctcctgcgcg accaggaggc gaacgcgccc tggcagcggc tgagcacctc
     1561 gctactgctg ctgctgcaac tggcaatcgc ggcggtgttg atcctggccc tggctcgccc
     1621 ctggtcggtg gccgcgacgc cgggcggcag caacctggtg atcgtgctcg acacctcggc
     1681 cagcatgggc gcgaccgatg gccccgccgg gcgcacgcgg ctggctgagg ccgtgcgcga
     1741 cgccggggcg cgcatcagcg ccctgccggc caacggcacc gccgcgctga tcgccgcggg
     1801 cacccatgcg cgcatcgtca gcccggccac gggcgaccgc gacgcgctgc ggcgcgccct
     1861 cgacacgctg caaccggccc cgaccacgac cgacctgagc gaggccctca gcctcgcggc
     1921 aggcgtggcc gccgcgagcg ccgaccccga agtgctggtc tattcggacg gccagttccc
     1981 cgacccgcgc ggcaccgtcc cggcgctggc cgcgccgctg cactttgtcg ccatgggcac
     2041 gcggggggcg aaccaggcca tcgtcgccct gagcgtgcgg cgcacggcgg ccacgctgag
     2101 cctgctggcc caggtcgcca acgccgacac cgcgcaggtc acgcgccgcc tcgacatcgc
     2161 actcgacggc gtggcctggg ccgggcgcac gctgaccctg ccgcccggcg agacgacgca
     2221 ggtgctgttc gacgaggtgc cgcccgccgc ccaggtggtt caggcgcatc tcgccggggc
     2281 cgacgacctg gcggtggacg acacggcctg gacgatcaac cacgccgccg aacccgcccc
     2341 ggtgctgctg gtgacggacg gcaaccgctt cctgcaaaac gcgctcgtcc tgctgcccaa
     2401 cgtcgccctg gaccacaccg ccccggcgga ctactcgccc gcactgaccg ccacgctgac
     2461 cgtcttcgac cgcacggtgc ccagcaccac gctgcccacg ggcaacctgc tgttcgtggc
     2521 cccgcccacc tccacgacca tcttcgaggt cacgggcgtg ctgaccacgc cgaacccgct
     2581 gccccaggtc gcgccggcct cggtcgatcc cggcacggcg ccggcggacg gggcaggcgg
     2641 cgacccgctg ctgcgttata ccgacctgag cgacctgcac atcgcccgcg cggcgcatat
     2701 cgcgcgcccg ccgtgggcgc gcgtcgtcct cgacagcgac gccgggccgc tgatcctggc
     2761 cggggaggtg gaggggcatc gggtggtgat catcggcttc gacctgcacg acagcgatct
     2821 gccgttgcag atgtcgtatc cgttgctgat gcgtaacctg gtcggctacc tgctgccgga
     2881 ggcagcaggc gacctggcgg ccagcgtcca gcccggcgac ccggtgccgc tggccgccgc
     2941 cgtgcaggag ggcgtggacc ggctgacggt gcgcggtccc accggcgcgc aattggcgag
     3001 ctatgccgtg ggtcccgacc agttccgctt caccttcacc ggggccgata cgccgggggt
     3061 ctacatcgtc agccagtggg tgggcgaccg cgaggtgcgc cgcgagggct tcaccgccaa
     3121 cctcttctcg ctggacgagg cgcggacggc cccgcgcgcc gatccgccgc tgcccgccgg
     3181 gcggccggtg ggcgccgccg acgtatccga cgtgcaaacc gggcaggggc agttcgagtg
     3241 gtggcagggc ctggccgcgc tggccttgct cgcgctgatc gccgaatggc tgatcaccca
     3301 ccggctgggg ctgcgccaac tgggccgccg cttgcgcggg cggcccctgg aggatccgct
     3361 tggacattcg tgagtttgcc tgggatgact acgacgccgt gtatgcccta tgggaacggc
     3421 tgcacatgct caagccgagt atcgacagcc gcgtggcact ggccgggtgc ctggagcgca
     3481 atccgggcct gttcctggtc gccgtggcgc ccgacgggca cccggcgggc acggtgctgg
     3541 gcacgttcga cgggcggcgc ggctacctgt accatgtcgc ggtcgatccc gcctaccggc
     3601 gccagggcat cgggcgcgcg ctgatgaccg agatcatgga ccggttgcgg gcgcgcggcg
     3661 ccgtgaagat cacgctgcgc gtggcccgcg agaaccaaag cgccatcgcc ttttaccgca
     3721 gcgtgggcct cgacgccgac gagcacgttt tgggcatgag catcgagtat tagccgcaac
     3781 cgccgggcgc ccggacccgg cgggttgagg cggcggtgga tcgcatcgcc gcggaccgca
     3841 agttcgcgtg gaagaggggc gtcatggacc tatcgtgggc ggggctgccg ctgctgttta
     3901 ccgacccctg gtggctggtc gcgtttgccc tgctgccccc gctgatcgtg ctggggctgt
     3961 gggaggggcg caccgtgcgc cggcggcggg tggcgctggg cgtggtgcgc gccgggccgg
     4021 agcgctggcg ctcgcgggtc agtgccatcg tgcgggcgac gattctcagt tgcgtgctgc
     4081 tggccctggc cgggctgcaa ctggtgcgca gcgccgggcc ggtgagcacg gtctacctgg
     4141 tggacgtgtc ggacagcgtg agcgcgaacg accgggcggc ggcgcacgcc tatgtgcagc
     4201 gggcgctgga cgccgcgcgg tccgacgagc gctccgccgt cgtgctgttc gccgggcagg
     4261 cccaggtggt gcgcccgttc gccgccacgg ggccgctggc ggccttcgac gcccaattgc
     4321 ccgcgggcgg caccgacatc gcggcggcgc tgcgcctggg cacggcgctg ctgcccgccg
     4381 acggcccgcg ccgcctggtg ctgatcagcg acgggcagga gaccgacgag caggcccgca
     4441 ccgaggccac cacggcagcc gccgccggca tccccgtcag cgtcgtgccg ctgcacagcg
     4501 gggcgctgaa cgaggtcgcg gtggacgccg tgggcgcgcc gagcatggtg ccctccggac
     4561 agggcttcga gatccaggtg cgcttgacca gcacggcggc ggagggcgcg cacctgcgcc
     4621 tgttccagga cgacacgctg ctggccgagc gcgatgtgag cctgcaaaaa ggcagcaacc
     4681 tgttcacgtt cccggccacc gcgcgcgacg agggcttcca cagctaccgc gccgtggtca
     4741 gcgcggtgga cgaccgctgg gcgcagaaca acgaggccgc cgcggtcacg gtggtgcaaa
     4801 gcccgccgcg cgtgctcatc gtggccgggc agccggacga cgccgagccg ctgcgcgtgg
     4861 cgctggaagc ggcgcacatc gagccgcagg tggcgaaggc cgccgacgtg ccgcacacgg
     4921 tggacggcct ggccgcctac gacagtatcg tgctggcgaa cacgcccgcc ggcgccctcg
     4981 gcgacgcgac gctggccgcc atgcaggcct acgtgcgcga
//
LOCUS       DASUYC010000046         3853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1631630, whole genome shotgun sequence.
ACCESSION   DASUYC010000046 DASUYC010000000
VERSION     DASUYC010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1631630"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="VF276_00720"
     CDS             complement(<1..746)
                     /locus_tag="VF276_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX6605413.1"
                     /translation="MISDTMPVVYDRPAALTPALTHYCPGCTHGVIHRLVAEVLDELH
                     LRERTIGVASVGCSVFAYNYFDCDFASAPHGRAPAMATAIKRVHPDRVVFTYQGDGDL
                     ASIGAGEILHAAARGENITVIFVNNAVYGMTGGQMAPTTLPGMRTTSSPLGRDPVQAG
                     WPVRMSELLAQVDGAAYVTRQSVHDPGHINRTRKAIRTAFQAQLHEAGFAMVEVLSSC
                     PVNWDLTPKEALRFVAEKMVPYYPLGEFKVT"
     gene            complement(803..1876)
                     /gene="vorB"
                     /locus_tag="VF276_00725"
     CDS             complement(803..1876)
                     /gene="vorB"
                     /locus_tag="VF276_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HEX6605414.1"
                     /translation="MTRELWKGNEAIAEAAVRGGLTAYFGYPITPQTELLEYLARRMS
                     ELGRVFVQAESELAAINMVYGAACAGARTMTSSSGPGISLMQEGFSYIAASEVPCVVV
                     DIMRGGPGLGNLAPTQADYFQLTKGGGHGDYHPLVLAPSSVQEAADLTYQAFDIAEHY
                     RTIVIIAADGTIGQMMEPVVMPPMRTIWPQDHGWELTGAVGRERRMITSLRLDPAALE
                     ELNDNLQAKERAIRETEVRYDERWTDDAELIVVAFGSAARIAQTAVTAARQRGIRVGM
                     FRPISLWPFPAAALAALTQRVPRVLVLELNAGQMLEDVRLAAGSAAHIAFMGRMGGLV
                     PLPDEILAQIQLLAPRAAVGLPG"
     gene            complement(1873..2163)
                     /locus_tag="VF276_00730"
     CDS             complement(1873..2163)
                     /locus_tag="VF276_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEX6605415.1"
                     /translation="MRGTIVVDESRCKGCELCTSVCPKDLIHLADYFTSRGYRPAVLQ
                     DPGGLCTGCLLCSTICPDVAITVYREAARRPVISARVPAADAPVPVLVGGAP"
     gene            complement(2473..3243)
                     /locus_tag="VF276_00735"
     CDS             complement(2473..3243)
                     /locus_tag="VF276_00735"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX6605416.1"
                     /translation="MDATMLTLKATGKEAPRLRTAQIVALHLAPGLLATPAYIGLAPV
                     VTGAGAPPALALLLVSLFVLLPVELGYLLYQGRRRNGRWAPAGVVLYRAPLARRQYLL
                     LVPALLVWSAVSSTLLTPLDEAIRQSLFAGLPDWFFATNFVQNLGQYSRESLVAVLLL
                     SAVLNIAVPAVEELYFRGYLLPRMAGAGRWAPLLNVALFSLYHLWTPWDNPARIVALL
                     PVVYAVWWQRNIYLSMAVHCALNTIGTLGLLALLITRL"
     gene            complement(3346..>3853)
                     /locus_tag="VF276_00740"
     CDS             complement(3346..>3853)
                     /locus_tag="VF276_00740"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6605417.1"
                     /translation="TQFGILVGAYGLAMVFGQAVLGRLSDRVGRKPVIAAGMLLTMLF
                     YLGLATQDQFVPMVATALLAGLGIALATPALSAFYLDITAAAHRSRVMGLKESAAALG
                     GVAGPLAVALFSRWLPPQTIFIIAAVLMAAGAALVVALLKEQRRSRTATQAAAPPVGA
                     GTMPEPTA"
BASE COUNT          658 a         1337 c         1301 g          557 t
ORIGIN      
        1 gtgactttaa actcgccgag cgggtagtaa gggaccatct tctcggccac aaaccgcaac
       61 gcctctttgg gcgtcaggtc ccagttcacg ggacacgacg agagcacctc gaccatcgcg
      121 aagcccgctt cgtggagttg cgcctggaac gcggtgcgga tggctttgcg ggtgcggttg
      181 atatggccgg ggtcatgcac cgattggcgc gtgacgtagg cggcgccgtc tacctgggcc
      241 agcagttcgg acatgcgcac gggccacccg gcctgcaccg ggtcccgccc cagcggcgag
      301 gacgtggtgc gcatgcccgg cagcgtcgtg ggcgccattt gcccgcccgt catgccgtag
      361 accgcgttgt tgacgaagat gacggtgata ttctcgcccc gcgcggcggc gtgcaagatc
      421 tcccccgcgc cgatggacgc cagatcgccg tcgccctggt aggtaaacac cacgcggtcg
      481 ggatgcaccc gcttgatcgc cgtcgccatg gccggcgcac gcccatgcgg cgccgaggcg
      541 aagtcacagt cgaaatagtt gtaggcgaac accgaacagc ccaccgaggc cacgccgatg
      601 gtgcgttcgc gcaggtgcaa ctcgtccagc acctcggcga cgaggcgatg gatgacgccg
      661 tgcgtgcagc cggggcagta gtgggtcagc gccggggtca gcgccgcggg ccggtcgtag
      721 acgaccggca tcgtgtcaga gatcatgaga cagctccttt ccggcacgcg ccggggcaac
      781 ctcgttgttg cgccgtggcc gatcagccgg gaagcccgac cgcagcccgc ggagccagca
      841 attggatctg ggcgagaatt tcatcgggca gcggcaccag cccgcccatg cggcccatga
      901 aggcaatgtg cgcggcgctg cccgcggcca ggcgcacgtc ttcgagcatc tggcccgcgt
      961 tgagttcgag caccagcacc cgcggcaccc gctgcgtcag cgcggccagc gccgccgccg
     1021 ggaacggcca caggctgatg ggccggaaca tgcccacgcg gattccgcgc tgccgggcgg
     1081 ctgttaccgc cgtctgcgcg atgcgcgccg ccgagccgaa cgccaccacg atcaactcgg
     1141 cgtcgtcggt ccagcgctca tcatagcgca cctcggtttc gcggatggcc cgttccttgg
     1201 cttgcaggtt gtcgttcaac tcctctagcg ccgccggatc gagccgcagc gaggtgatca
     1261 tgcgccgttc gcggcccaca gcgccggtca gttcccagcc gtggtcctgc ggccagatcg
     1321 tgcgcatcgg cggcatgacc accggctcca tcatctggcc gatggtgccg tcggcggcga
     1381 taatgacgat ggtgcgatag tgctcggcga tgtcgaacgc ctgataggtc aggtccgctg
     1441 cttcctggac cgacgaaggg gccaggacga ggggatgata gtcgccgtgg ccgccgccct
     1501 tggtgagctg gaagtagtcg gcctgcgtgg gcgccaggtt gcccaggccg ggtccgccgc
     1561 gcatgatgtc gaccaccacg cagggcactt ccgacgccgc gatatacgag aacccttcct
     1621 gcatcagcga gatacccggt cccgacgacg aggtcatggt ccgcgcgccg gcgcaggccg
     1681 cgccgtagac catgttgatg gccgccagct cgctctcggc ctggacgaac acgcgcccca
     1741 gttccgacat gcgtcgcgcc aggtactcca acaactcggt ttgcggggtg atcggatagc
     1801 cgaaataggc ggtcagcccc ccccgcacag ccgcttcggc gatggcctcg ttgcccttcc
     1861 acaactcccg tgtcatggcg cacctcctac cagcaccggg accggggcgt ctgccgccgg
     1921 aacgcgagcg ctgatgaccg ggcgccgcgc cgcctcgcgg tagaccgtga tcgccacgtc
     1981 gggacagatc gtcgagcaga gcaagcaacc ggtgcataaa ccgcccggat cctgcaggac
     2041 cgccgggcgg taaccgcgcg aggtgaagta atcagccagg tgaatcaagt ctttggggca
     2101 gaccgaggta cagagttcgc agcctttgca gcggctctca tcgacgacga tggtaccgcg
     2161 catggttaca cctccttcgc aggctgctca ggcccggcgc cgggatggac gatcatggac
     2221 ctgagccggc ggtatcctgg ccgaacgggg tctcatgatc ctgtgccgcc gtggaacagg
     2281 atgccggcga ggcccgccct ccgcctcagg atggcgccac cctgattcta aggcggtggc
     2341 gtgaaatgcg cggtatagag aggtgaaaaa ttgatgacaa atgcgccgcc gcgggtgcag
     2401 ccgccggggc cggggggcac ggtgccgcct gccgggccgt gccccgccgc cgtcatcgcg
     2461 ggtgacgccg gctcatagcc gggtgatcaa caacgccagc aggcccagcg tgccgatcgt
     2521 gttcagcgca cagtgtaccg ccatactcag gtagatgttc cgttgccacc acacggcgta
     2581 gaccacgggc aacagcgcga cgatccgcgc cgggttatcc cacggcgtcc acaggtggta
     2641 gagcgagaac agcgcgacgt tgagcagcgg cgcccagcgc cccgcgcccg ccatgcgcgg
     2701 caacaggtag ccgcggaagt agagttcttc gacggcgggc acggcgatgt tgagaaccgc
     2761 gctcaacagc agcaccgcga caagcgactc gcgcgagtat tgccccaggt tctgcacgaa
     2821 attggtcgcg aagaaccagt ccggcagccc ggcgaacagg ctttgccgga tcgcctcgtc
     2881 gagcggagtc agcagcgtgg acgatacggc gctccacacc aggagcgcgg gcaccagcag
     2941 cagatactgc cgccgcgcca ggggtgcgcg ataaagcacc acgcccgccg gcgcccagcg
     3001 cccgttgcgc cgccggccct ggtagagcag atagcccaac tccacgggaa gcagcacgaa
     3061 cagcgagacc aacaacagcg ccagggccgg cggcgcgccc gcgccggtga cgacgggcgc
     3121 cagcccgata taagccggcg tggcaagcag gcccggcgcc aggtgcagcg ccacgatttg
     3181 cgctgtccgc aagcgcgggg cctctttgcc ggtagccttg agggtgagca ttgtagcatc
     3241 cacgggaatt ctccttcgcc tacccggata cgctgtcgct gccgcccggc gtgcggggcc
     3301 ggtttgctac cgctccccac cgtaccgggc ggcagcgacg ctgccttacg cggtcggttc
     3361 gggcatcgtg cccgcaccca ccggcggcgc ggccgcttgc gtggcggtgc ggctgcggcg
     3421 ctgctctttc agcagggcca ccaccagcgc ggcgcccgcc gccatcagca ctgcggcgat
     3481 gatgaagatc gtctgcgggg gcagccagcg gctgaacaac gctaccgcca gcgggccggc
     3541 cacgccgccc agcgccgccg ccgactcctt gaggcccatc acccgcgacc ggtgcgcggc
     3601 cgccgtgatg tcaaggtaaa acgcactcaa cgcgggtgtt gccagggcga tgcccagccc
     3661 ggccagcaac gccgtggcga ccatcgggac gaactgatcc tgcgtcgcca ggcccaggta
     3721 gaacagcatg gtcagcagca tcccggcggc gatcacgggc ttgcgcccga cccggtcgct
     3781 cagccggccc aggaccgcct ggccgaatac catcgccagc ccataagcgc ccacgaggat
     3841 gccgaactgc gtg
//
LOCUS       DASUYC010000047         2248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_323674,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000047 DASUYC010000000
VERSION     DASUYC010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323674"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VF276_00745"
     CDS             complement(<1..280)
                     /locus_tag="VF276_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605418.1"
                     /translation="MREFGTLALLCGLLLFAALAQIAPGGRGMAAPVAETAALHSDSG
                     DPGGPVAADDDLIAQLAAGAPPARPGLPQMAPRTPTGDSIDLIPPGPIP"
     gene            complement(281..1171)
                     /locus_tag="VF276_00750"
     CDS             complement(281..1171)
                     /locus_tag="VF276_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX6605419.1"
                     /translation="MLDDAIEDLPRDMAPALPTGWERAWWTPMNYALATLVGLLFIVP
                     LVWMISTSLRPIGLPAPARFEWIPPVLTLDNYPYIFQILELGRFLGNSIGVVVVAVPL
                     TLLTASAAGFALAQLPPRPRGWLTALSVAALLVPAMALWITRFLVYKWIGILDTPLAL
                     IAPAIMGTSPLYVLLFGWAFGRVPGEIFEQARLDGAGAWRIWWSVALPLVRPAVGAVA
                     ILSMVFYWSDFISPLLYVNNQAYYTLPVGVQALQQVNRTNWPLMMSGAVVLTVPMLIL
                     FIAAQRFFFQEDRNQSWWGR"
     gene            complement(1164..2075)
                     /locus_tag="VF276_00755"
     CDS             complement(1164..2075)
                     /locus_tag="VF276_00755"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX6605420.1"
                     /translation="MLRLSVYHRQLLVLLAPYLLGMLVLIIIPTLLAAPIAFTEYDAL
                     SPPRFVGLDNFGKILADPQFRNGLTASLWLVVIAVPLRVLGALGLALLLRARKRGFTV
                     FRGLAYLPTIIPDVAYGLVWLYIFNPLFGPLNNLSYIFQGTIETEFSPGALPGIWLTD
                     PRAAQAAVIIMLLFTIGEGFVLLLASLNEIPAELYEAAALDGAAGDQQVRHITLPHLA
                     PSLLLLSLRDTIFIFQASFVAAVIVTKGGPYYATTYLPYWIYINITEFQRFGYGAAMT
                     WVLYIITALVIALQFWVARRWRGMQYA"
BASE COUNT          437 a          706 c          799 g          306 t
ORIGIN      
        1 gggggatagg ccccggcggg atgagatcga tggaatcgcc ggttggcgtg cggggcgcca
       61 tctgcggcag gccgggcctg gccggcggcg ccccggcggc cagttgggca atcaggtcgt
      121 cgtccgcggc aaccgggccg ccgggatcgc cgctatcgct gtgcagcgcc gcagtctccg
      181 ctaccggcgc ggccatgccg cggccgccgg gcgcgatctg cgccagggcg gcgaacagca
      241 gcaacccgca gagcagggcc agcgtgccga actcgcgcat ctagcggccc caccagctct
      301 ggttgcgatc ttcctggaag aagaaccgct gggccgcgat gaacaggatc aacatcggca
      361 cggtcagcac cacggcgccg gacatcatca gtggccagtt ggtgcggttc acctgctgca
      421 atgcctgcac ccctaccggc agcgtgtaat acgcctggtt gttcacatac agcagcgggc
      481 tgatgaagtc gctccagtaa aaaaccatgc tcagaatggc gacagcgccg acggccgggc
      541 gaaccagcgg cagcgccacc gaccaccaga tgcgccaggc gcccgcgccg tcgaggcggg
      601 cctgctcgaa gatctcgccc ggcacgcggc caaaggccca gccaaagagc aagacataga
      661 gcgggctggt gcccatgatc gccggggcaa tcagcgccag tggcgtatcg aggatgccga
      721 tccacttgta gaccaggaag cgcgtgatcc agagcgccat agccgggacg agcagtgccg
      781 cgaccgagag cgcggtcagc cagccgcgcg ggcggggcgg caactgggcc agcgcgaacc
      841 cggcggcaga cgccgtgagc agcgtcagcg gcacggccac gacgaccacg ccgatggagt
      901 tgcccaggaa gcggcccagt tcgaggatct ggaagatgta gggatagttg tccagtgtga
      961 ggacgggcgg gatccactcg aagcgcgcgg gcgcgggcag gccgatgggc cgcagcgagg
     1021 tgctgatcat ccagacgagc ggcacgatga acagcaggcc caccagtgtc gccagggcgt
     1081 agttcatcgg cgtccaccac gcgcgctccc agccggtcgg cagggccggc gccatgtcgc
     1141 ggggcaggtc ctcaatcgcg tcgtcaagca tattgcattc cccgccaccg tcgcgccacc
     1201 cagaactgca gcgcgatgac cagcgccgtg atgatgtaga gcacccaggt catggccgct
     1261 ccgtagccga agcgttggaa ctcggtgata ttgatataga tccagtaggg caggtaggtg
     1321 gtggcgtaat acggcccgcc cttggtgacg atgacggcgg cgacgaagct ggcctggaag
     1381 ataaagatcg tgtcgcgcag gctgagcagc agcagcgagg gcgccaggtg cggcagggtg
     1441 atgtggcgca cctgctggtc gcccgccgcg ccgtccagcg ccgccgcttc gtagagctcc
     1501 gccgggatct cgttcagcga ggccagcagc agcacgaagc cctcgccgat ggtgaacagc
     1561 agcatgatga tgacggcggc ctgggccgcg cgggggtcgg tgagccagat gccggggagc
     1621 gcgccggggc tgaactcggt ttcgatggtc ccctggaaga tgtaggagag gttgttgagc
     1681 ggcccgaaca gcgggttgaa gatgtagagc cagacgaggc cataggccac atcggggatg
     1741 atcgtgggca ggtaggccag gccgcggaac acggtaaagc cccgcttgcg cgcgcgcagc
     1801 agcaaagcca accccagcgc gcccagcacc cgtaacggca cggcgatcac caccagccag
     1861 agcgacgcgg tcagcccgtt tcggaactgc ggatcggcca ggatcttgcc gaaattgtcc
     1921 aggccgacga agcgcggcgg ggagagcgca tcgtactcgg tgaaggcgat gggcgcggcc
     1981 agcagcgtgg ggatgatgat cagcaccagc atgcccaaca aatagggcgc cagcagcacc
     2041 aggagttggc gatggtagac ggacagacgc agcatattgc cttatgatag catatggctc
     2101 aggccctgat caaggcgacg gcccggcggc ttgcgctgcc ggctagcttt acgacggcgg
     2161 tctgggcggc ctcatcaccc acccgcagcc cgcttgcgct tttcggcgct agaatgtacc
     2221 atgcgtcacg aaggagtctt gtgcatga
//
LOCUS       DASUYC010000048         5436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1706693, whole genome shotgun sequence.
ACCESSION   DASUYC010000048 DASUYC010000000
VERSION     DASUYC010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5436
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1706693"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VF276_00760"
     CDS             <1..471
                     /locus_tag="VF276_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX6605421.1"
                     /translation="DDGVQCRCGNYGCLETVVSTPAVCRRALDLARTHRDSLLNQADV
                     PPGEMPIEAVVRVFQAGDPTAQQIVRELGHYLGIAASQLVGTLNVRHIALVGDLTLFG
                     EPLAAAIRAEMLRRALPQLAQATEVALLDESPDVVILGAAALLLARELGLNLAR"
     gene            728..1771
                     /locus_tag="VF276_00765"
     CDS             728..1771
                     /locus_tag="VF276_00765"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX6605422.1"
                     /translation="MTAREGSRNLLVGVDAGGTKVAVLVADAQGSPRGRATYPTDLSG
                     PEQTLAGIAAAIRVTLAVAGVPLDAVAAVGLGVPGRVDPETGVVRHAVNLKWEALPVG
                     DLLGGLLGVPCFVENDVRAAALGLQRHPAHAGVQHLAYLSVGTGIAAGVILNGRLHRG
                     AHGMAGEIGHMIVDPAGPRCECGSRGCLEALTAGPAIARLGAQAVAAGADTVLRAAAP
                     LTAEAVYQAAGAGDAVAQAITGQVGRRLAQALQQLIMSYDVERVVLGGGVARAGDLFL
                     GPILAEFGRLRAESALAAEMLPPQMFHLLPPDYDAGTWGAVTLAREGLNRVHSAVVDS
                     TEGGSQREPVHTG"
     gene            1914..3254
                     /locus_tag="VF276_00770"
     CDS             1914..3254
                     /locus_tag="VF276_00770"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6605423.1"
                     /translation="MRTKIMLLLMVLSALLAACGSEPAATPTAVPAAPPAATTAAQPA
                     ATTAPASSGQTTIKVMLVDYIKDKTDKWLQDEAIPAFQKAHPNIVVQPIYVTWSTLDE
                     TVQGYYTAGDGADILNLGSEYIAQYGDRLADLNQYLGEAAWPDIKQYLPSTLATVTWQ
                     GKLRGLPWLTAPRAYMCRTDLAPSSTTFKDAIANAKAGTKADGGAVTQAGLVTTGRLD
                     DWQEWIELIWALGGQLYKDDGTPQFDSAEAKAALQFMYDRRRAVYATETLADLPEAKG
                     SRLVDGTAACVWGSTWAGPPTGDALWSKIDLQPSPIDATAFPKAKPVVQVFNDWLAVP
                     SYSKHVAEAAEFLKFLGSAENQNRYNADFGSFPPRSDAWTGYVAKDPVLQKMGALMQQ
                     YGVGFADIRESAKLREVLQKEMPAFFTDQQDLATTLANIQKQYTQVLKDAGRLK"
     gene            3319..4464
                     /locus_tag="VF276_00775"
     CDS             3319..4464
                     /locus_tag="VF276_00775"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX6605424.1"
                     /translation="MSDSYLTTLLWAAGTVIFIIAASLALGSLARWIVRRRGASQGLQ
                     DRTFAGYLFAGPWIIGFVIFVVAPMLASLYWSFTTFKPPAQAEWVGAQNYLRVLTEDK
                     DFRSALFNSLFLTVVGLPLQLGVALALAVLLNQRLRGERIFRMAFYLPVVLGLNAAAL
                     LCWRLMLNANNGIVNSALRGLGAIFEPFNWLTRTFIYAMEVANAFFLGLQNGNMGPLN
                     GVLKAGFPAPERVPLWLQNPLWTKTSIILIMIWSCGTMMLIYLAALYNIPREMQEAAE
                     VDGASTWQRFRHVTLPLITPATFYNLVVGTIATIQIFEQTYVLFRDTPTVAQSAYSVV
                     YYLWRATFRFNEIGYGSAISWLLLVLILVITLVQFRLQDRWVQYDLR"
     gene            4477..5322
                     /locus_tag="VF276_00780"
     CDS             4477..5322
                     /locus_tag="VF276_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX6605425.1"
                     /translation="MTRRKQGWLWRPVFYVLAITGSVLMAFPLLWMIATSFKSEAEAN
                     APRIVWLPSKPQLDAYIRILTDAAFQRSYLNSTFVTVLALLGTLISIAAVAYAFSRIE
                     WPGRNVVFLLMLSTMMIPAQALIVPQYVFFNAARWIGSFNPITIPGFFAGGAALIFLL
                     RQFMLQIPSEVDEAAHIDGANYFQIWLYIILPLSKPALATVGTFLFVGTWNSLLNPVI
                     YLQSSDLYTLPIYVASLVNPQQTSQPWPTIMAASVLTTIPLIVLFIFAQRFLLQSIAL
                     SGSKG"
BASE COUNT          867 a         1848 c         1826 g          895 t
ORIGIN      
        1 gacgacggag tgcagtgccg ctgcggcaac tacggctgcc tggaaacggt ggtcagcacc
       61 ccggcggtgt gccgccgcgc actcgacctc gcccgcacgc accgcgactc gctgctgaac
      121 caggcggacg tgccgccggg cgagatgccg atcgaagccg tggtgcgggt cttccaggcc
      181 ggcgacccca cggcgcaaca gatcgtgcgc gaactgggcc actacctggg catcgccgcc
      241 tcgcagttgg tcggcacgct gaacgtgcgg cacatcgcgc tggtgggcga cctgaccctg
      301 ttcggcgagc ccctggcggc ggctatccgc gccgagatgc tgcggcgggc gctgccacaa
      361 ctggcccagg ccacggaggt ggcgctgctc gacgagagtc ccgatgtggt gatcctgggc
      421 gcggcggcgt tgctgctggc ccgcgaactg gggctgaacc tggcccgtta gcgtcattcc
      481 ggcaacttat cggggatagc acaggcgaaa tacgcacgag gacggcgcga aaaaacgcgc
      541 ggcggcactc gtagggccgt gctgtgccaa agacggacgc atacggcgcg attcatcgcg
      601 cggctacaca tatggagacg tgacgcgctc aagacggacg cgtacggcgc gatgaatcgc
      661 gcggctacgg atagcggaca acgggatctg cgtgagcgga ccattgctgg aagtggagac
      721 aacggtagtg acggcacgtg aggggtcgcg gaacttgctg gtcggcgtgg acgcgggcgg
      781 caccaaggtg gcggtgctgg tggccgatgc ccagggcagc ccgcggggcc gcgccacgta
      841 cccgacggac ctgagcggcc cggagcagac gctggcgggc atcgccgccg ccatccgggt
      901 gacgctggcc gtggcaggcg tgccgctgga cgcggtcgcc gccgtcggcc tgggcgtgcc
      961 aggccgggtc gatcccgaga cgggcgtggt ccggcacgcg gtcaacctga agtgggaagc
     1021 gttgccggtg ggcgacctgc tcggcggact gctgggcgtg ccctgcttcg tggagaacga
     1081 tgtgcgggcg gcggcgctgg ggttacagcg ccacccggcc cacgcgggcg tgcaacacct
     1141 ggcctacctg agcgtcggca cggggatcgc cgcgggcgtg atcctgaacg ggcggctgca
     1201 tcgcggcgcg cacggcatgg ccggcgagat cgggcatatg atcgtggacc cggcggggcc
     1261 gcgctgcgaa tgcggctcac gtggctgcct ggaggccctg acggcgggtc cggccatcgc
     1321 ccggttgggc gcgcaggccg tggccgcggg cgcggatacc gtcttgcgcg ccgctgcgcc
     1381 gctgacggcg gaagccgtct accaggccgc gggcgcgggg gacgccgtgg cccaggccat
     1441 caccgggcag gtgggccgcc ggctcgccca ggccctgcaa cagttgatta tgtcctatga
     1501 cgtcgagcgc gtcgtccttg gcgggggcgt ggcgcgcgcc ggcgatctgt tcctgggccc
     1561 gatcctggcg gagttcggcc ggctacgcgc ggaatcggcc ctggccgcgg aaatgttgcc
     1621 ccctcagatg ttccacctcc tgccgccgga ctatgacgcc ggcacctggg gcgccgtgac
     1681 cctggcacgc gagggcctga atcgcgtcca ctccgcggta gtggacagta ccgaaggagg
     1741 ttcgcagcga gaaccggtcc ataccgggta ggcgccccgc gcgaggcggc gggacgcccg
     1801 gcccggtggg cacccggccc ccggctactg acgggcctgg ggcgggcgat tcttccacga
     1861 gtcataccct ggcgacagca catcttccat ttcacagtag gaggagcgca ccgatgcgta
     1921 cgaaaatcat gttgttgctg atggttctca gcgcgttact cgccgcgtgc ggcagcgaac
     1981 cggctgccac acccaccgcc gttcccgcgg ccccgcccgc cgccaccacc gcggcgcagc
     2041 ccgccgccac caccgccccg gccagcagcg gccagacgac catcaaggtc atgctggtcg
     2101 actacatcaa ggacaagacc gacaagtggc tgcaagacga ggccatcccc gccttccaga
     2161 aggcccaccc caacatcgtc gtccagccga tctacgtgac ctggagcacg ctcgacgaga
     2221 ccgtgcaggg ctactacacc gccggcgacg gcgccgacat cctcaacctc ggctcggagt
     2281 atatcgccca gtacggcgac cggctggccg atctcaacca gtacctgggc gaagcggcct
     2341 ggcccgacat caagcagtac ctgccctcga ccctggcgac cgtgacctgg cagggcaagc
     2401 tgcgcggcct gccctggctg acggcgccgc gcgcctacat gtgccgcacg gacctggcgc
     2461 cgagttccac caccttcaaa gacgccatcg ccaatgccaa ggccggcacc aaggccgacg
     2521 gcggcgccgt gacgcaggcg ggcctggtta cgaccgggcg gctggacgac tggcaggagt
     2581 ggatcgagct gatctgggcg ctcggcggcc agctttacaa ggacgacggc acgccgcagt
     2641 tcgattcggc ggaggccaag gccgcgctgc agttcatgta tgaccgccgc cgcgccgttt
     2701 atgccactga aaccctggcc gacctgcccg aagccaaggg gtcgcgcctg gtggacggca
     2761 ccgccgcctg tgtctggggc agcacctggg ccggcccgcc gaccggcgat gccctctgga
     2821 gcaagatcga cctgcagccg tcgcccatcg acgcgaccgc cttccccaag gccaagccgg
     2881 tggtgcaggt gttcaacgac tggctggccg tgccgtccta ctccaagcat gtggccgaag
     2941 ccgccgagtt cctgaagttc ctcggcagcg ccgagaacca gaaccgctac aatgccgatt
     3001 tcggctcgtt cccgccgcgg tccgatgcct ggaccggcta cgtggcgaag gacccggtgt
     3061 tgcagaagat gggcgcgctg atgcagcagt acggggtcgg gttcgccgac atccgcgaat
     3121 cggccaagct gcgcgaagtc ttgcagaagg agatgcccgc gttcttcacc gatcagcagg
     3181 acctcgccac gacgctggcg aatatccaga agcaatacac gcaagtgctc aaggatgccg
     3241 ggcgcctgaa gtaaggccgg cgcagggatc cggatggggc cgcccggccc cgtccggacc
     3301 cacttcgggg agtagaccgt gagcgattcg tacctgacca cgctgctgtg ggccgcgggc
     3361 accgtgatct tcatcatcgc cgccagcctg gcgctgggga gcctggcccg gtggatcgtg
     3421 cgccggcggg gggccagcca gggcttgcag gaccgcacct tcgccggcta cctgttcgcc
     3481 gggccgtgga tcatcggctt cgtgatcttc gtggtggcgc ccatgctggc ctcgctctac
     3541 tggagtttca ccacgttcaa gccgccggcc caggccgaat gggtgggcgc gcagaactac
     3601 ctgcgcgtgc tgacggagga caaagacttc cgctcggcgc tgttcaactc gctgttcctg
     3661 accgtcgtgg ggctgccgct gcaactaggg gtggcgctgg ccctggccgt gctgctgaac
     3721 cagcggctgc gcggcgagcg catcttccgc atggccttct acctgccggt ggtgctgggg
     3781 ctgaacgcgg cggcgctgct ctgctggcgg ctgatgctca acgccaacaa cggcatcgtc
     3841 aactcggcgc tgcgcgggtt gggcgccatc ttcgagccgt ttaactggct cacgcgcacc
     3901 ttcatctacg cgatggaggt cgccaacgcc ttcttcctgg ggctgcaaaa cggcaatatg
     3961 gggccgctga acggcgtgct caaggcgggc ttcccggcgc cggagcgcgt gccgctctgg
     4021 ctgcaaaacc cgctctggac caagacctcg atcatcctga tcatgatctg gagttgcggc
     4081 acgatgatgc tgatttacct ggccgcgctc tataacatcc ccagggagat gcaggaggcg
     4141 gcggaagtgg acggcgcgag cacctggcag cgcttccgcc atgtgacgct gcccctgatc
     4201 accccggcca ccttttacaa cctggtcgtc ggcaccatcg cgacgatcca gatcttcgag
     4261 cagacctacg tgctgttccg cgacacgcca acggtggccc agtcggccta ctcggtcgtc
     4321 tactatctct ggcgggcgac cttccgcttc aacgagatcg gctacggctc ggccatctcc
     4381 tggctcttgc ttgtgctcat tctcgtgatt accctggtcc agttccggtt gcaagatcgc
     4441 tgggtccagt acgatctgcg ataggagtag cgtgccatga cccgcaggaa gcaaggatgg
     4501 ctctggcggc ccgtgttcta cgtcctggcg atcacgggca gcgtgctgat ggccttcccg
     4561 ctgctctgga tgatcgccac gtcgttcaag agcgaggccg aggccaacgc gccgcgcatc
     4621 gtctggctgc ccagcaagcc gcaactcgac gcctatatcc gtattttgac cgacgccgcg
     4681 ttccagcggt cgtatctcaa ctcgaccttc gtcaccgtgc tggcgctgct gggcacgctg
     4741 atctcgattg ccgccgtggc ctatgccttc agccgcatcg agtggccggg ccgcaacgtg
     4801 gtcttcctgt tgatgctgtc cacgatgatg atcccggcac aggcgctgat cgtgccgcag
     4861 tacgtgttct tcaacgcggc gcgctggatc ggcagcttca acccgatcac catccccggc
     4921 ttcttcgcgg gcggcgcggc gctgatcttc ttgttgcgcc agttcatgct gcagatcccc
     4981 agcgaggtgg atgaggcggc gcacatcgac ggcgcgaact acttccagat ctggttgtac
     5041 attatcctgc cgctgagcaa gcccgccctg gcgaccgtcg gcacgttcct gttcgtgggc
     5101 acctggaact cgctgctgaa cccggtgatc tatctgcaat cgtccgacct ctacacgctg
     5161 ccgatctacg tggcctcgct ggtcaacccc cagcagacga gccagccctg gccgacgatc
     5221 atggccgcct cggtgctgac cacgatcccg ctgatcgtgc tgtttatatt cgcccagcgc
     5281 ttcctgctgc aaagcatcgc gctttccggg tctaaaggat gatgacaccc ttgacgacga
     5341 caccgcaact caccctggac caggcggcgg gccagaagct catggccgcc tttgttggct
     5401 acgaaccgtc cgcgcagatc ctggggctat tgcagc
//
LOCUS       DASUYC010000049         8496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_550812,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000049 DASUYC010000000
VERSION     DASUYC010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 8496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8496
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550812"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1286)
                     /locus_tag="VF276_00785"
     CDS             complement(<1..1286)
                     /locus_tag="VF276_00785"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX6605426.1"
                     /translation="MAATDSISPTGAGDSLDAIFRAMESVRRAETLEAAVQALFDLSR
                     LLLAGHAGYAALYLVSEHAGGDAAPAPAELLSLRAAYPPDKAATALPPRAILAEAAFA
                     TAPRTWAPAEAATWVLLPLGTGAATGTLAVLIDAPPDDAALARLQIVAGLAAQAGELV
                     LLRGQAGRGIAEMSLLDRISAVVSSSLSLDEILTESLAELARIMPFEGGSIALINKAQ
                     ELEIRAMCGVIDAEARQVRVPVGAGISGWVAAHGRPYLSNDLDAEQGVRPVMRSTGTN
                     RKIKAYMAVPLTVEGRVIGILQVNSPAKHAFTGRDLALLAEVGERCAVAIERARLFDE
                     MQARANRLASVTEMARFISASLDPDELFRICYEQVRKVMPADAFLVALYDAEAAAVRY
                     EFVVDMGEVYPKYSKPLDAGLTSYVIRTRRPLRVGQR"
     gene            1627..3435
                     /locus_tag="VF276_00790"
     CDS             1627..3435
                     /locus_tag="VF276_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605427.1"
                     /translation="MHRSPARVRPLMLLTLLTLLVAPLAGLSGAQPAHAQATVPSTWT
                     KGFVMIGYGPDPYKISNVPASLAALKATGANTVALAPIWFMDNGSSTTMGPKPEAGTP
                     SDESTVAAIREAHRLGLRVMMRPYIDVSDGTWRADITPRDVNAWFANYTAFINHFLDM
                     SRAEGVEEFTIGVELINMTDPQYDAKWRALIAQSRARYSGKLTYSANWGKHTRNEYKQ
                     ITFWDALDYIGLSAYFPISLKDNPTTEEMARGWSNYTDQVGQTYNWISEIKATADQYH
                     KPVVFTEIGFGSYSNSPGRWDVTQHVNTLSLETQQRAVEATFQAFVGQPWFQGIYWWH
                     WEPYALGGGAKDLSDTLNNKPAEATITRWFGGSGQPQPGPQPQPPPQPQPGPPPGVTP
                     RPAPAPGQAGVNNPAFNAVPSPGPDTATAVYFAATGHKLGGGFLDYWKNHGGLAMFGY
                     PISEEYWEINPTDGKPYLVQYFERQRFEYHPEAPAGSQVLLGLLGVALVNGRNTGPFA
                     VVPSPGPDTAARVFFAPTGHTLSDRFLRYWQQYGGLAMFGYPLSEPFQEVSTTDGKTY
                     TVQYFERARFEYHPENSGSFYEVLLGFLGRWYAGKQ"
     gene            3631..5958
                     /locus_tag="VF276_00795"
     CDS             3631..5958
                     /locus_tag="VF276_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605428.1"
                     /translation="MPIPMHADQSPPAGRRARLAVFLVAALLAVLPLLGLAATAPRPS
                     QAAPAQAEFSPNLRLNQAQTDQQQEPSLSINPKDPRMIIAAAKDWRSGRKEVWHYHSS
                     DGGATWTDLHLPGLPADLPNQSDPVVIYDADGVAYTSIIGYNDSDFTRGGLYVARSTD
                     NGATWSTPVAAAKNSDRVFNDKEWLTVDRSNTATRGTLYLTWTRFTTVSEHQDRGDIV
                     EVRSTDGGTTWSEPVQVSRAQDQTDVQGSFPAVGPGGELTVLYYDQTKNAALWAARST
                     DGGKNFAPPVKVADAHRIPSPLPTSKFRTFILPSLAANPLDGTLVATWNDYTTNADIL
                     LATSRDGNQTWSPPVRVNQDTGTADQFFPSAVFGPDGVLHVAWLDRRDDPKNLTFSCY
                     YTQSTDDGATFAPNVRISDAQSDPSVGFGGTLIGDYIQVDAVAGRAWVAWIDTRTGDQ
                     NIYGASISGSLGPGTPPGTPAATPTPGGATATPTPVPGLNFVDPAFARVWERSDQPVA
                     SHVAARPWMWGPAPFAAGNESYQQGPNGRRLVQYFDKSRMEINNPAADPAQKWYVTNG
                     LLVVEMMTGKIQVGDAEYDTRTYTPNAEPVAGDPGSPDAPTYASLARVASLNGENRAE
                     DRTGQIISATIDAAGNVTAATPPLAAAAQVANARYEPKVGHNIPNVFVDFMNQQGPIY
                     VNGQVVQGPVVDPLFDLGYPITEAYWARLRIAGQDHWALIQAYQRRVLTYVPDNAPPW
                     QVEMGNVGRHYYDWRYGPNSPYAAERGDMDADEGE"
     gene            complement(6128..7360)
                     /locus_tag="VF276_00800"
     CDS             complement(6128..7360)
                     /locus_tag="VF276_00800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6605429.1"
                     /translation="MPRSSGVVFFYPRLKKLTGAERLILKLADYVSRAGMPVTLLTHR
                     LAAACRPDLAPQVRLRQTGRRLEWTGRHYADAALEYLLGPWLLRGLPPLREVRGLVFF
                     GPPSLPALWWAKRVLLPWHGRRDIPCLYFCFEPPRVIYSDTAAISRRLGPAGVALRLL
                     ARVHKGVDRRMVAAADALLGNSPFGAELLARAYGRPAVVLPHGVDFVPPTPAQVAAAR
                     ARIALGDRPLAITVNHLHPRKRVDMFLRALALARQAGHDVGGVIVGLGPERAALEALA
                     GELGLQGHVHFAGFVPEADLPAYYRAADIYVHTGLLESFGLSVIEAAALGLPVVAVAE
                     GGPTATVRDGETGLLVASTPEAVAAGLGRLAGDADLRRCMGEAAAAYIARTYRWQDGA
                     RVFLDQLAAARRRLGARG"
     gene            complement(7422..7592)
                     /locus_tag="VF276_00805"
                     /pseudo
     CDS             complement(7422..7592)
                     /locus_tag="VF276_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
     gene            complement(7783..8427)
                     /locus_tag="VF276_00810"
     CDS             complement(7783..8427)
                     /locus_tag="VF276_00810"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABATE domain-containing protein"
                     /protein_id="HEX6605430.1"
                     /translation="MNKSAPDPTQLKLIGGRPCLDFVNTVGSRLNQTGAPFEWLTSYE
                     GLLIWCRHAGILPDDTVATLLEQAESQADEAAAVLARAQALREALYRIFVAVAQDRDP
                     APADLAILNAVLAATLGRRRLVSTATGIDWTWVDPSGALDAMLGPLAESAASVLTSSD
                     VARVRQCGDDECAWMFVDTSRNHSRRWCDMEDCGNRAKARRHFHRVRQSSAAGS"
BASE COUNT         1429 a         3104 c         2778 g         1185 t
ORIGIN      
        1 cgctggccga cccgcagggg tcgccgtgtg cggatgacgt agctggtcag gcccgcgtcc
       61 agtggcttgc tgtacttggg gtagacctcg cccatatcga ccacgaactc gtagcgcacc
      121 gccgccgcct cggcatcgta caacgcgacc aggaaggcat cggcgggcat caccttgcgc
      181 acctgttcgt agcagatgcg gaacagttcg tcgggatcga gcgaagccga gatgaagcgc
      241 gccatctcgg tcacgctcgc cagccggttg gcgcgcgcct gcatctcgtc gaacagccgc
      301 gcgcgctcaa tcgccacggc gcagcgctcg cccacctcgg ccagcaaggc caggtcccgg
      361 cccgtgaacg catgtttggc cgggctgttg acctgcaaga tgccgatcac ccggccctcc
      421 acggtcagcg gcacggccat atacgccttg attttgcggt tggtgccggt cgagcgcatc
      481 accgggcgca ctccctgctc ggcgtccagg tcgttgctga ggtagggccg gccatgggcc
      541 gccacccagc ccgagatgcc ggcgcccacc ggcacgcgca cctggcgggc ctccgcgtcg
      601 atcacgccgc acatggcgcg gatctccagt tcctgcgcct tgttgatcag cgcaatcgac
      661 ccgccttcaa acggcataat ccgcgccagt tcggccaggc tctcggtcag gatctcatcc
      721 aggcttaggc tggaactgac cacggcggag atgcgatcca gcaggctcat ctcggcgatg
      781 ccgcgcccgg cctggccgcg cagcagcacc agttcgcccg cctgggccgc cagcccggcc
      841 acaatctgca agcgggccag ggcggcgtcg tccggcggcg cgtcgatcag gacggccagg
      901 gtgccggtgg ccgcgccggt gcccaggggc agcaagaccc aggtggcggc ctcggcgggc
      961 gcccaggtgc gcggcgccgt ggcgaaggcc gcctcggcca ggatggcgcg gggcggcagc
     1021 gcggtcgccg ctttgtccgg cggatacgcc gcgcgcaaac tcagtaattc agcaggcgcc
     1081 ggcgccgcat ccccgccggc gtgctcgctc acgaggtaga gcgccgcgta gccggcgtgc
     1141 ccggccagga ggaggcggct gagatcaaag agggcctgga ccgcagcctc cagcgtctcg
     1201 gcgcggcgca cgctctccat cgcacggaaa atcgcgtcga gcgaatcgcc ggcgccggtg
     1261 ggcgagatgg aatcggtggc ggccaaaggg atcctcccgg ataggtcgcg gtgcgcagcg
     1321 gagcctgaac tgttaaccgg atcggtgaat atattataga acgccgcgcc cctatccacc
     1381 gttaccccgg cccggcgccc gcgtggaagc cgggttcccg tcgccccgcc ttgcgaccgc
     1441 ctaaaccatc ctggtatact tagccgatac ggcatccggc catccccaaa aagggtgtaa
     1501 cggcacaacc tattttgcag caggttcgtt ttaaagatga acccggcgcc cggcgtggcc
     1561 ggtcgtatca gggtgcgcgc tgcgcgcgcg ccggcaaccc gacacacact gagaggagct
     1621 tttttgatgc atcgatcccc cgcccgcgtg cgtcccctga tgctgctcac gctgctgacc
     1681 ctgctggtcg cgccgctggc cggcctgagc ggggcccagc cggcccacgc ccaggcgacc
     1741 gtgccgagca cctggaccaa gggcttcgtg atgatcgggt acggccccga tccctacaag
     1801 atcagcaacg tgccggcgtc gctcgccgcg ctgaaggcca cgggcgccaa taccgtggcc
     1861 ctggccccga tctggttcat ggacaacggc agctccacca cgatggggcc gaagcccgaa
     1921 gcgggcaccc cgtccgatga gtccactgtg gcggccatcc gcgaggcgca ccggctcggc
     1981 ttgcgcgtca tgatgcgccc gtacatcgac gtgagtgacg gcacctggcg cgccgacatc
     2041 accccgcgcg acgtgaacgc ctggtttgcg aactataccg ccttcatcaa ccacttcctg
     2101 gatatgtcgc gggccgaggg cgtcgaggag ttcaccatcg gcgtcgagtt gatcaacatg
     2161 accgacccgc agtatgacgc caaatggcgg gcgctcatcg cgcagagccg cgcccgctat
     2221 tccggtaagc tgacctactc ggccaactgg ggcaagcaca cccgcaacga gtacaagcag
     2281 atcaccttct gggacgcgct cgattacatc ggcctgtccg cctacttccc catctcgctg
     2341 aaggacaacc cgaccaccga ggagatggcg cgcggctgga gcaattacac cgaccaggtg
     2401 ggccagacct ataactggat cagcgagatc aaggcaaccg ccgaccagta ccacaagccg
     2461 gtggtcttca ccgagatcgg cttcgggtcc tacagcaatt cgccgggccg ctgggatgtc
     2521 acccagcacg tgaacacgct gagcctggag acgcagcagc gcgcggtcga ggcgacgttc
     2581 caggcgtttg tgggccagcc ctggttccag ggcatctact ggtggcactg ggagccgtat
     2641 gccctgggcg gcggcgccaa ggacctgagc gacacgctga acaacaagcc cgccgaagcg
     2701 acgatcacgc ggtggttcgg cggcagcggc cagccgcagc ccggcccgca gccgcaaccc
     2761 ccgccgcaac cgcagcccgg cccgccgccc ggcgtgacgc cgcgcccggc ccccgcgccc
     2821 ggccaggccg gggtgaataa ccccgccttc aacgccgtgc cctcgcccgg ccccgacacc
     2881 gccacggctg tctacttcgc ggcgacgggg cataagctgg gcggcggctt cctcgactac
     2941 tggaagaacc acggcgggct ggcgatgttc ggctacccga tctccgagga gtactgggag
     3001 atcaacccga ccgacggcaa gccctacctg gtgcaatact tcgagcgcca gcgcttcgag
     3061 taccaccccg aggcccccgc gggcagccag gtgctgctcg gcctgctggg cgtggccctg
     3121 gtgaacggcc gcaataccgg cccgtttgcc gtcgtgccct cgcccggccc cgacaccgcc
     3181 gcgcgcgtct tcttcgcgcc caccggccac accctgagcg accgcttcct gcgctactgg
     3241 cagcagtacg gcgggctggc gatgttcggc tacccgctca gcgagccgtt ccaggaggtc
     3301 tcgaccacag acggcaagac ctacacggtg cagtacttcg agcgcgcccg cttcgagtac
     3361 cacccggaga acagcggcag cttctacgaa gtgctgctgg gcttcctggg ccgctggtac
     3421 gccggcaagc agtaacgcgc actccgcaca cacgcagagg cggcccatcg gggccgcctc
     3481 tgtctatctg aaccccggtg ccggcgcccc cggtaatcta catgagcggg gccggcagcg
     3541 gccctacctg caatagccgc gatctgttag aatgcagcaa gcacgccgca tacaccgggc
     3601 aacggataac cgacctagcg ctagatggag atgccgattc ccatgcacgc cgatcaatcg
     3661 cccccggcgg gccgccgcgc ccgcctcgct gtgttcctcg tcgccgcgct gctggccgtt
     3721 ctgccgctgc tgggcctggc ggcaaccgcc ccgcgcccca gccaggccgc gcccgcccag
     3781 gccgagttca gccccaatct gcgcttgaac caggcgcaga ccgatcagca acaagagccg
     3841 agcctgagca tcaaccccaa ggacccgcgc atgatcatcg cggcggccaa ggactggcgc
     3901 agcggccgca aggaagtctg gcactatcac tccagcgacg gcggcgccac ctggaccgac
     3961 ctccatctgc cgggcctgcc cgccgatctg cccaaccaga gcgacccggt ggtcatctac
     4021 gatgccgacg gcgtggccta caccagcatc atcggctata acgactccga tttcaccagg
     4081 ggcggcctct acgtcgcgcg ctcgaccgac aacggcgcca cctggagcac cccggtggcg
     4141 gcggcgaaaa atagcgacag ggtcttcaac gacaaagaat ggctgacggt ggaccgcagc
     4201 aacaccgcca cgcggggcac gctctacctg acctggaccc gctttaccac cgtctccgag
     4261 caccaggatc gcggtgatat cgtcgaggtc cgttccaccg acggcggcac cacctggagc
     4321 gaaccggtgc aggtcagccg cgcccaggac cagaccgatg tacaggggtc cttcccggcg
     4381 gtcgggccgg gcggcgagct caccgtgctc tactacgacc agaccaaaaa tgccgcgctc
     4441 tgggccgcgc gcagcaccga cggcggcaag aactttgccc cgccggtcaa ggtggccgat
     4501 gcccaccgca tcccctcgcc cctgcccacc agcaagttcc gcacgttcat cctgccctcg
     4561 ctggccgcca acccgctcga cggcaccctg gtcgccacgt ggaacgacta cacgaccaac
     4621 gccgacatcc tgctggcgac ctcgcgcgac ggtaaccaga cgtggagccc gccggtgcgc
     4681 gtcaaccagg acaccggcac ggcagaccag ttcttcccct cggcggtgtt cgggcccgac
     4741 ggcgtgctgc atgtggcctg gctggaccgg cgcgacgacc ccaagaacct gaccttctcc
     4801 tgctactaca cgcagtccac cgacgacggc gcgaccttcg cccctaatgt ccgcatcagc
     4861 gacgcccaga gcgaccccag cgtcggcttc ggcggcacgc tgatcggcga ctacatccag
     4921 gtggacgccg tggccgggcg ggcctgggtg gcctggatcg acacgcgcac cggcgaccag
     4981 aacatctacg gcgcgagcat cagcggcagc ctggggccgg gtacgccgcc cggcacgccc
     5041 gccgccaccc cgacgcctgg cggcgccacg gccacgccta ccccggtgcc ggggctgaac
     5101 ttcgtggacc cggccttcgc gcgcgtctgg gaacgcagcg accagccggt ggcatcgcac
     5161 gttgcggccc ggccctggat gtggggaccg gcgcccttcg ccgcgggcaa cgaatcctac
     5221 cagcaaggcc ccaacggccg ccggctggtg cagtatttcg acaagagccg catggagatc
     5281 aataaccccg ccgccgaccc ggcgcagaag tggtacgtga ccaacggcct gctggtggtc
     5341 gagatgatga ccggcaagat ccaggtgggc gacgcggagt acgacacgcg cacctacacg
     5401 cccaacgccg agccggtggc gggcgacccc ggcagccccg acgcgcccac ctacgcctcg
     5461 ctggcccgcg tggcctcgct gaacggcgaa aaccgggccg aagatcgcac cggccagatc
     5521 atcagcgcga cgatcgacgc cgcaggcaac gtcaccgcgg ccacgccgcc gctcgccgcc
     5581 gcggcccagg tggcgaatgc gcgctacgag ccgaaggtgg ggcacaacat ccccaacgtc
     5641 ttcgtcgatt tcatgaacca gcaggggccg atctacgtga acggtcaggt ggtacagggg
     5701 ccggtggtcg atccgctgtt cgacctcggc tacccgatca ccgaagccta ctgggcgcgg
     5761 ctgcgcatcg ccgggcagga ccactgggcg ctgatccagg cgtaccagcg ccgcgtgctg
     5821 acctacgtgc ccgacaacgc cccgccctgg caggtggaga tgggcaacgt gggccgtcac
     5881 tactatgact ggcgctacgg ccccaactcg ccctacgccg ccgaacgcgg cgacatggac
     5941 gcagacgagg gcgaataacc ccgccgcttg tgacactgag cggagcgaag agtctcgtct
     6001 aggctgattc cgcaccgcga aggaacgaag gaaacgaaga ttcttgatat aagcgttatt
     6061 cttcgtttcc ttcgttcctt cgcggtgaat ttatgcacgg atgccggaca gggccgcagc
     6121 ccgcacccta accccgcgcg cccaaccgcc gccgggcggc ggccagttgg tccaggaaca
     6181 cccgcgcgcc gtcttgccag cgataggtcc gggcgatgta cgcggcggca gcctcgccca
     6241 tgcagcgccg gaggtccgca tcgcccgcga gccgccccag gcccgccgcc accgcttccg
     6301 gcgtgctcgc caccagcagt cccgtctcgc cgtcgcgcac ggtggcggta gggccgccct
     6361 cggccacggc gaccaccggc aggcccagcg cggcggcctc gatcactgag aggccaaagc
     6421 tttccagcag gcccgtgtgg acgtagatgt cggcggcgcg gtagtaggcg ggcaggtcgg
     6481 cttcgggcac gaacccggcg aaatggacat gcccctgcaa gcccagttcg ccggccaggg
     6541 cttcgagcgc ggcccgctcc ggccccaggc ccacgatcac cccgcccacg tcgtggccgg
     6601 cctggcgggc cagggccagg gcgcgcagga acatatcgac gcgcttgcgc gggtgcaggt
     6661 gattgaccgt gatcgccagc gggcggtcgc ccagggcgat gcgggcgcgc gcggcggcca
     6721 cttgcgccgg ggtaggcggc acgaagtcca cgccgtgcgg cagaacgacc gccgggcgcc
     6781 cgtaggcgcg cgccagcagt tcggccccga aggggctgtt gccgagcagc gcgtcggcgg
     6841 cggcgaccat gcgccggtcc acgcctttgt gcacccgcgc cagcagccgc agggcgaccc
     6901 ccgccgggcc gaggcgccgg ctgatcgccg cggtgtcgct gtagatgacg cggggcggct
     6961 cgaagcagaa atagaggcag ggaatatcgc gccgcccgtg ccagggcagc agcacccgct
     7021 tggcccacca cagcgcgggc aggctgggcg gcccgaaaaa gaccaggccc cgcacctcgc
     7081 gcagcggcgg tagcccgcgc aagagccacg ggccgagcag gtattccagc gcggcgtcgg
     7141 cgtagtggcg gcccgtccac tccagccgcc gcccggtctg gcgcaggcgc acctggggcg
     7201 ccaggtcggg ccggcaggcg gcggccagcc ggtgcgtcag cagcgtcacc ggcatgcccg
     7261 cccggctcac gtagtcggcc agtttgagga tcagccgctc ggcgccggtc agttttttta
     7321 ggcgcggata gaaaaagacg acgccgctac tgcgcggcat cggcggcgcc gggcgtgcga
     7381 ccgctgctgt cgggctgcgc atcggcttca ccctcgcggg tctgccgcgc ctcttccacc
     7441 agcatcaccg ggatgccgtc gcgcaccggg taggcgcgcc cgcattcggc gcacaccagt
     7501 tcctcgccct gcaacgtcac cttcgcgtga tcgaccgggc agaccagaat atcgagcagg
     7561 tcctggggta tttcgttcgt ggtcgcgtcc atagctcctc ctgggaatcg gcaaattcca
     7621 cgcgcccgcg ggcccgtgta ccggcattat acgccaaatc gcggcaatcg ggccaggggc
     7681 cggcctaacc cccttgcccc cttcccggcg cgggaaaggg gaaaaccgac tcccctcccc
     7741 gccgcgggga ggggctgggg gtgaggttgg ttcggatgcg cgtcagctcc ccgcagcgct
     7801 gctctggcgg acgcggtgga agtggcggcg cgccttggcc cggttgccgc agtcttccat
     7861 gtcgcaccag cggcggctgt ggttgcggct ggtgtccacg aacatccagg cgcactcgtc
     7921 gtcgccacac tggcgcaccc gcgccacgtc ggaggacgtg agcacgctcg ccgccgactc
     7981 ggccagcggg ccgagcatcg cgtccagcgc gccggacggg tcgacccagg tccagtcgat
     8041 gcccgtagcg gtggagacca gtcgccgccg gcccagcgtg gcggccagca cggcgttgag
     8101 gatcgccagg tccgccgggg ccgggtcccg atcctgggcc acggcgacga agatgcggta
     8161 gagcgcctcg cgcaacgcct gcgcgcgggc cagcacggcg gcagcctcgt ccgcctgact
     8221 ctcagcctgt tcgagcaagg tcgccacggt atcgtcgggc aaaatcccgg cgtgccggca
     8281 ccagatcaac aacccctcat agctggtcaa ccattcaaac ggcgcaccgg tctggttcag
     8341 gcgactgccc acggtattga caaaatcgag gcacggccgc ccaccgatca gcttcaactg
     8401 cgtcgggtct ggggcggatt tgttcatcgg atctccttct atagtcctag actataacca
     8461 ccaataggtc cttgacaggt tatacaaggc aggcta
//
LOCUS       DASUYC010000050         2291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1342672, whole genome shotgun sequence.
ACCESSION   DASUYC010000050 DASUYC010000000
VERSION     DASUYC010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1342672"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VF276_00815"
     CDS             complement(<1..311)
                     /locus_tag="VF276_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605431.1"
                     /translation="MNGFEQTPEKPRHSLRRLSDYDYAQAGAYFVTLVTVDRALLFGT
                     IADGAMCLSSYGQIAHDVWADLPQHYPHITLDSFVVMPNHIHGVVFFADAAAVGAGLK
                     PA"
     gene            complement(320..>2291)
                     /locus_tag="VF276_00820"
     CDS             complement(320..>2291)
                     /locus_tag="VF276_00820"
                     /inference="COORDINATES: protein motif:HMM:NF024295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 44 protein"
                     /protein_id="HEX6605432.1"
                     /translation="VKVTYGEGWGAFSVADWKARNNADSRKYSHLHFWINGGPDGGQR
                     IGVSLGGDRVVLSNYLPDKTLAANRWQEVFIPLDDLQAGGQPIGRLTFQETAGKPQSP
                     YYLDGIEFYKDNTPPPTPILTTAQVTVDLSARRQPISPFIYGMSQAPEDVARAANITV
                     NRMGGNPYSRYNWTLGNARNAGSDAEFRNYQREPDDPAYRQPSGLADMFFKADQRTGA
                     ATLLTVPMLGYVAKNADNSTRSTNVPQEGGAPIAPGSEAIAGYDPKANQDATSVKSYP
                     RKGAPFQDPPDPNAGAVYQDEWINHLTRTFGKAVDGGVRFYAMDNEPDLWADDTHVDV
                     HPTRVGYDELLQKFMDYASAVKAVDPTAEVTGPVVSGWTGYWFSALDRGDDSFGTAAD
                     RAAHGGMPLIPWFLDQVHQRDQAGGRRTLDVLDIHYYPQAAGLAQDKTDPQTAALRLR
                     STRALWDPTYEDESWIAHTEAKQVNLIPRMLAWIDQYYPGTKLGITEWKWYAETTMNG
                     ALAIADVLGIYGREGVYLANYWTNPPLNSPGLEAFKLYRNYDGKQGTFGDVSVAATSS
                     APDKLAVYASEDTATHQVKIMVLNKAPQEQIATSLAVQGASLGGSAKVYHLSEATNLT
                     IAPQPAVPVTGGSLRYTFPAYSATLLVIDKGQ"
BASE COUNT          352 a          699 c          804 g          436 t
ORIGIN      
        1 gccggtttca aaccggcccc tacagcagca gcatctgcaa agaagacgac accgtgaata
       61 tggttcggca tgacgacgaa ggaatccagc gtgatatggg gatagtgctg ggggagatct
      121 gcccacacgt cgtgggcaat ctgtccatat gacgataggc acatcgcacc atcggcgatg
      181 gtgccaaaca ggagtgcacg gtctacggtt acaagcgtga cgaagtaggc gccggcctgg
      241 gcataatcat agtccgataa ccgccgtagg ctgtggcgtg gtttttcggg cgtttgctcg
      301 aatccgttca tgctaaggcc tattgcccct tgtcgatcac cagcagcgtg gccgagtagg
      361 ccgggaaggt gtagcgcagg ctgccgccgg tcacgggcac ggcgggctgc ggcgcgatgg
      421 tcaagttcgt ggcctcgctg aggtggtaga ctttggcgct gccgccaagg ctggcgccct
      481 gcacggccag gctggtcgct atctgctcct ggggcgcttt gtttagcacc atgatcttga
      541 cctggtgcgt ggcggtgtcc tcgctggcgt agacggccag cttgtcgggc gccgacgagg
      601 tggcagccac cgagacatcg ccgaacgtgc cctgcttgcc gtcgtagttg cggtagagct
      661 tgaaggcttc caggcccggc gagttgagcg gtgggttggt ccagtagttc gccaggtaca
      721 cgccctcgcg gccgtaaatc cccagcacgt cggcaatcgc cagcgcgcca ttcatcgtcg
      781 tctccgcgta ccacttccac tcggtgatgc ccagcttcgt gcccggatag tactggtcga
      841 tccaggccag catgcgcgga atcaggttga cctgcttcgc ctccgtgtgc gcaatccacg
      901 actcgtcctc atacgtgggg tcccagaggg cgcgcgtcga gcgcaggcgc agcgccgccg
      961 tctgcgggtc ggtcttgtcc tgggccaggc ccgcagcctg cgggtagtag tggatgtcga
     1021 gcacgtcgag cgtgcgccgc ccgccggcct ggtcgcgctg atgcacctgg tcgaggaacc
     1081 aggggatcag cggcatgccg ccatgggcgg cgcggtcggc ggcggtgccg aagctgtcgt
     1141 cgccgcggtc cagcgccgag aaccagtagc cagtccagcc ggagaccacc gggccggtga
     1201 cttccgccgt cgggtccacg gctttcaccg cgctcgcgta gtccataaac ttctgcagca
     1261 gttcatcata gccaacgcgc gtgggatgca cgtccacgtg cgtatcgtcg gcccagaggt
     1321 cgggctcgtt gtccatggcg tagaagcgca cgccgccgtc caccgccttg ccgaaggtgc
     1381 gcgtcaggtg gttgatccac tcgtcctggt aaacggctcc ggcattagga tcgggcgggt
     1441 cctggaaggg cgcgcccttg cgcgggtagg acttcacgct ggtggcgtcc tggttggcct
     1501 tgggatcgta gccggcaatc gcctcggagc cgggcgcgat gggcgcgccg ccctcctgtg
     1561 gcacattggt cgagcgcgtg ctgttgtcgg cgttcttcgc cacgtagccc agcatcggca
     1621 cagtcaacag cgtggcggcg ccggtgcgct ggtcggcctt gaagaacata tcggccaggc
     1681 cagacggttg ccggtaggcg gggtcgtcag gttcgcgctg gtagttgcgg aattcggcgt
     1741 cgctgccggc gttgcgggcg ttgcccagcg tccagttgta gcgcgaatag gggttgccgc
     1801 ccatgcggtt gacggtgatg ttggccgcgc gggccacgtc ttcgggtgcc tgggacatgc
     1861 cgtagatgaa cgggctgatc ggctggcgcc tggcgctcag gtccaccgtg acctgcgccg
     1921 tggtcaggat cggcgtgggc ggcggggtat tgtctttgta gaactcaatg ccgtcgaggt
     1981 agtagggtga ctggggcttg cccgccgtct cctggaaggt gagccgcccg atgggctggc
     2041 ctccggcctg caagtcgtcg agcgggatga agacctcctg ccagcgattg gccgccaggg
     2101 tcttatccgg cagatagttg gagaggacga cgcggtcgcc gcccagggac acgccgatgc
     2161 gctggccgcc gtcggggccg ccgttgatcc agaagtgcag atggctatac ttgcgcgagt
     2221 cggcgttatt gcgggccttc cagtcggcaa cggagaaagc gccccagccc tcgccatacg
     2281 tcaccttgac c
//
LOCUS       DASUYC010000051         5935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1937303, whole genome shotgun sequence.
ACCESSION   DASUYC010000051 DASUYC010000000
VERSION     DASUYC010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5935
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1937303"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..3712)
                     /gene="metH"
                     /locus_tag="VF276_00825"
     CDS             complement(131..3712)
                     /gene="metH"
                     /locus_tag="VF276_00825"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEX6605433.1"
                     /translation="MTESAYLAALRDRVLVFDGAMGTSVQRYDLDAEAFGGCMGCNDY
                     LPMTRPEVIEEIHASFMEVGCDVLETDTFGGNRLKLDEYGLGARTHEINFGAAQLARR
                     VADRYSTPDKPRFVAGSIGPTGMLPSSDDPTLSNITFDQLVEIFYEQSKALVEGGVDL
                     LIIETTQDILELKAAIIGANRYFKDSGRRVPIQAQVTLDVTGRMLLGTDIAAALTTLE
                     TLAVDVIGLNCSTGPEHMREPIRYLAEHCSKPISCIPNAGLPLNQNGQAVYPLEPVPM
                     AETLAEFVNELGVEVVGGCCGTTPAHLAEVVKRVGGKQRTPRQVTRTPAISSAMRSVD
                     LHQVPAPLIVGERVNAQGSRKVKRLLLANDYDGILGVAREQVEGGAHALDVTVALTER
                     TDEDAQMVAVIKKLRAGVEAPIVIDSTEANVIEAALKAYPGRPVVNSINMERGRERIE
                     AVVPNVVEHGAAVVVMPIDEVGMAKTADKKAEVCKRIFDICVNEYGLPADSIIFDVLT
                     FPVTTGQEDLVDAAIQTLEGIRQAKAACPGSLSILGVSNVSFGLTPHARAVLNSVFLH
                     HAVQAGLDLAIVHPSHVVPYTEVPAEQRGLADDLLLARRPDALPRYIAYFEENKPEAG
                     GQETAADPTEGMAVEEKIHWQILHRRKDGIEALLDEAITHQDPVGVLNNVLLPAMKDV
                     GDKFGAGELILPFVLQSAEVMKKAVAHLEQYFEKKEGYTKGKVVVATVFGDVHDIGKS
                     LVNTILTNNGYTVYDLGKQVPINVIIDRALEVQADAIGLSALLVNTSKQMPLCVQELA
                     KRNLEIPVLIGGAAINRNFGRRIGFVNEGERYTPGVYYCRDAFEGLDTMEALSGPERA
                     TFIERKHREARIAAEREVSEEELLAEAAARPKPARSPKVRTDVPIPTPPFWGWRVVDP
                     IPAADMFACLDLNSLFRLSWGAKSLKGERWDTLLRDDFLPRLERLKTELIQSRALQPR
                     MIYGYYPCQSAGDQVIVYDPADPQARREITRFTFPRQPDRDFLALSDYFADVDSGRID
                     VIGLQCVTVGDRVSELTAEWNRAGNYTEAYYLHGLSVACAEALAEYGQGRFREELGLD
                     PNQGRRYSWGYPACPDTMEHYRLLEALPFDRTIGVTLTTAGQLSPEQSTAALVTHHPQ
                     SKYYTTRPVRQLRNGAGGAGEDGDEGTDEAGAGPTAPLLIPAAAGVE"
     regulatory      complement(3889..3989)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4115..4708)
                     /locus_tag="VF276_00830"
     CDS             complement(4115..4708)
                     /locus_tag="VF276_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605434.1"
                     /translation="MSFNSSACRARNPALPLPVRATALRSCVMRLCRLTGERYTHALA
                     RYGDALGIDLTNVTEQELVTVLARIESERNQILSKVRAFEQQRVQDKQRGNRRLAKED
                     AATFQALRDPTGLAPDPARPREPVPRKNRWSAEQWRRFAVALVAWAGIFFLGAIVVLM
                     CRFLGIIRPEEQSWVIPFVTGGCGLILGTIILVFKGR"
     gene            complement(5033..>5935)
                     /locus_tag="VF276_00835"
     CDS             complement(5033..>5935)
                     /locus_tag="VF276_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605435.1"
                     /translation="FCVVDNDYAGFPRTPVENLQVTAAHEYYHATQFAYDVADDGWFL
                     EATATWAEDLVYPTLDQEQSQLQDGGWARNNDGPLGPLDLFEDDGLAQYGAYIWPFYL
                     THRPGGDPTLIGQIYKASEHQAPIKALHAMPDWNARFKEFALWNWNWDPVDMYRDHGA
                     HIGPLAQSPVMLGDDGTLSLATASDQARVNLKETSIEYYEVARIDEGTNGGKPVRQLR
                     FDLSAVTGQAGAGVQAIITVGSGAAARRYTEDWSGLQTKTFCRDRSAEQVSKVVLVVT
                     NANIGARQTIRGRINVDAIRDACR"
BASE COUNT          919 a         1956 c         1960 g         1100 t
ORIGIN      
        1 cgcatcttcc ctactggttt atggccggcc tggcgccggc ttatttcgcc atctatcgct
       61 cctgtacaaa tagacaaaac gcagccgctc tccgatcaga gagcggctgc gctgttggcg
      121 gcggatgcgg ctactcgact cctgccgccg cgggtatcag cagcggggcg gtggggccgg
      181 cgcccgcttc gtctgtgccc tcgtcgccgt cctccccggc tccgcccgcg ccgttgcgta
      241 gctggcgcac gggccgggtg gtgtagtact tcgactgcgg gtggtgggtg accagggccg
      301 ctgtgctctg ctcggggctg agttgcccgg cggtggtcag cgtgacgccg atagtgcggt
      361 cgaagggcag cgcttccagc aaacggtagt gctccatggt gtccgggcag gccggatagc
      421 cccaggagta gcggcggccc tggttcgggt cgagacccag ttcctcgcgg aagcggccct
      481 gcccgtactc ggccagcgcc tcggcgcagg ccacgctgag gccgtgcagg tagtaggctt
      541 cggtgtagtt gcccgcccgg ttccactccg ccgtcaactc gctcacccgg tcgcccacgg
      601 tcacgcactg caagccgatc acgtcgatgc gccccgagtc cacgtccgcg aagtagtcgc
      661 tgagggccag gaagtcgcga tcgggctggc gcgggaaggt gaagcgcgtg atctcgcgcc
      721 gcgcctgcgg gtcggcggga tcatagacga tgacctggtc gcccgcgctc tggcagggat
      781 agtagccgta gatcatgcgc ggctgcagcg cccggctctg gatcagttcg gttttcaggc
      841 gctccagccg gggcaggaag tcgtcgcgca gcaatgtatc ccagcgctcg cctttcaggc
      901 tcttggcgcc ccacgagagg cggaagagcg agttcaggtc gaggcaggcg aacatgtcgg
      961 cggcggggat aggatcgacc acgcgccagc cccagaaggg cggggtgggg atgggcacat
     1021 cggtgcgcac cttgggcgag cgggcgggct tgggccgcgc ggcggcttcg gccagcaact
     1081 cttcctcgga gacctcgcgc tcggcggcga tgcgcgcctc gcggtgcttg cgctcgataa
     1141 acgtcgcgcg ctccggcccg ctcagcgcct ccatcgtgtc caggccctcg aaggcgtcgc
     1201 ggcagtagta gacgccgggc gtgtagcgct cgccctcgtt gacgaagccg atgcgccgcc
     1261 cgaagttgcg gttgatggcc gcgccgccga tcagcacggg gatttcgagg ttgcgcttgg
     1321 caagctcctg cacacagagc ggcatctgct tcgacgtgtt gaccagcagc gcgctgaggc
     1381 cgatggcgtc ggcctgcacc tccagcgccc ggtcgatgat cacgttgatc ggcacctgct
     1441 tgcccaggtc atagaccgtg tagccgttgt tcgtgaggat ggtgttgacc aggctcttgc
     1501 cgatgtcgtg cacatcgccg aagaccgtgg cgacgacgac cttgcccttg gtgtagcctt
     1561 ccttcttctc gaaatactgc tccaggtggg cgacggcctt cttcatcacc tcggcggact
     1621 gcaacacaaa cggcaggatc aattcgcccg cgccgaactt gtcgcccacg tccttcatgg
     1681 cgggcagcag cacgttgttc agcacgccga ccgggtcctg gtgggtgatg gcctcgtcca
     1741 gcagcgcctc gatgccgtcc ttgcgccggt gcaggatctg ccagtggatc ttctcctcca
     1801 ccgccatgcc ctcggtggga tcggcggcgg tttcctgccc gcccgcttcc ggcttgttct
     1861 cctcgaaata ggcgatgtag cgcggcaggg catcggggcg gcgggccagc agcagatcgt
     1921 cggccagtcc gcgctgctcg gcgggcacct cggtgtaggg caccacgtgc gacgggtgga
     1981 caatcgccag gtccagcccg gcctgcaccg cgtgatgcag gaagacgctg ttgagcacgg
     2041 cgcgggcgtg cggcgtcagg ccgaacgaca cgttgctgac acccaggatg gacagcgagc
     2101 cggggcaggc ggccttggcc tggcggatgc cctccagcgt ctggatggcg gcgtcgacca
     2161 ggtcttcctg gccggtcgtg accgggaagg tcagcacgtc gaagatgatg ctgtcggcgg
     2221 gcaggccgta ctcgttcacg cagatgtcga agatgcgctt gcagacctcg gccttcttgt
     2281 ccgccgtctt cgccatgccc acctcgtcga tgggcatgac caccacggcg gcgccgtgct
     2341 cgaccacgtt gggcaccacg gcttcgatgc gctcgcgccc gcgctccatg ttgatcgagt
     2401 tgacgacggg gcggcccgga taggccttca gcgcggcctc gatgacgttg gcctcggtgc
     2461 tgtcgatgac gatgggcgct tccacgcccg cgcgcaactt cttgatcacg gccaccatct
     2521 gcgcgtcttc gtcggtgcgc tcggtcagcg ccaccgtcac gtcgagcgcg tgggcgccgc
     2581 cctcgacctg ctcgcgggcc acgcccagga tgccgtcgta atcgttcgcc agcagcaggc
     2641 gcttgacctt gcggctgccc tgcgcgttca cccgctcgcc gacgatcagc ggggcgggca
     2701 cctggtgcag gtccacggag cgcatggcgc tggagatggc gggggtgcgc gtcacctggc
     2761 gcggcgtccg ctgcttgccg ccgacccgct tgacgacctc ggccaggtgc gcgggggtcg
     2821 tgccgcagca gccgccgacc acctccacgc ctagctcgtt gacgaactcg gccagtgtct
     2881 cggccatggg caccggctcc agcgggtaga ccgcctggcc gttctggttc agcggcaggc
     2941 ccgcgttggg gatgcaggag atcggcttgc tgcagtgctc ggccaggtag cggatcggct
     3001 cgcgcatatg ctcggggccg gtcgagcagt tcaggccgat cacgtccacg gccagcgtct
     3061 ccagcgtggt cagcgcggcg gcgatgtcgg tgcccagcaa catgcgcccg gtcacgtcca
     3121 gcgtcacctg ggcctggatc ggcacccggc gcccgctgtc cttgaagtag cggttggcgc
     3181 cgatgatcgc cgccttcagt tcgaggatgt cctgcgtggt ctcgatgatc agcaggtcca
     3241 cgccaccctc gaccagggcc ttgctctgct catagaagat ctcgaccagt tggtcgaacg
     3301 tgatattgct cagcgtcggg tcgtcggacg agggcaacat gccggtgggg ccgatggacc
     3361 cggccacgaa gcgcggcttg tcgggcgtgc tgtagcggtc ggccacacgg cgggccagtt
     3421 gcgccgcgcc gaagttgatc tcatgggtgc gcgcgcccag cccgtactcg tccagcttga
     3481 gccggttgcc gccgaaggtg tccgtctcca gcacatcgca gcccacttcc atgaaagagg
     3541 catggatttc ctcgatgacc tcgggccggg tcatcggcag ataatcgttg cagcccatgc
     3601 agccgccgaa agcctcggca tccagatcat agcgctgcac cgaggtgccc atcgcgccgt
     3661 caaacaccaa tacccgatca cgtaaagccg ccagatacgc agattccgtc atgtttcacc
     3721 tacctaatta cctactgccc ttgcgggcac cgacgatcac tagggccatc cccggcccac
     3781 caagctaccc tgccgccgga tcacccacct accaggtaat gccgcgggca taaaaaaggc
     3841 ccctctaaga cgaggggcag gatgctaaca cggtagattc caaccactcc cttatctcac
     3901 agatcgctcc gtcggaattg gcacctgggc gggtccgcct ggttgccggg tttcatcggg
     3961 ccgtttccct ccacctctct tgataagagc ctggaatgct atgcaattgt aaagtgcctg
     4021 aaaattatag cacagccgcg cgggcactgt caagaatcgg cggatcgatg cgcctgccga
     4081 tggtttcggc gcttggtcta gccgaagtag ggactcagcg gcccttaaaa accagaatta
     4141 tcgttcccag gatcagtccg catcctccgg taacgaacgg gatgacccag ctttgctcct
     4201 ctgggcggat gatgcccagg aagcggcaca tgagcacgac aatggccccc aggaagaaaa
     4261 tgccggccca tgccaccagg gcgaccgcaa accgccgcca ctgctcggcg ctccagcggt
     4321 ttttccgcgg gaccggttcg cgaggacggg ccggatcagg cgctaaaccg gtgggatcgc
     4381 gcaacgcctg aaaggtcgcc gcatcctctt tcgccaggcg ccgattcccc cgctgtttat
     4441 cctgtacccg ctgctgctca aaagcacgca ccttgctcag gatctggttt cgttcgcttt
     4501 ctatgcgtgc caatacggtt acaagttcct gttcggtaac gttggtcaaa tcgatcccca
     4561 gagcatcacc gtagcgggcc agagcgtggg tatagcgctc tccggtcagc cggcataggc
     4621 gcatgacaca ggaccgtagc gccgtggcgc gcaccggcag gggcagtgca ggattccgcg
     4681 cccggcacgc tgacgagttg aaggacatgc ctttacccag gaagccgatc gcagagtcgt
     4741 gtatgcaccg agcttacaac ttctctggct tcgtggcata caactcggta taaccacaat
     4801 ggctgcatac tttcgcttcc agcgaggaca catcccagag ccggccacgt ccctcttttg
     4861 cttttatgcc caaggcgccg gtagttacaa gcgttgccgc tttcatcaac ccgccgcatt
     4921 tcgggcagct atcgtcagcc gttgggtcgt ccaggcgtcg ggtcttttga tcatccatcc
     4981 ttgctgctgg ttctttctct gttaatagcg gccaatcggc ccaggaacgg ccttaccggc
     5041 aggcgtcgcg gatggcgtcg acgttgatgc ggccgcggat ggtctggcgc gcgccgatgt
     5101 tggcgttggt cacgacgagc acgaccttgc tgacctgctc ggcggagcgg tcgcggcaga
     5161 aggtcttggt ttgcaggccg ctccagtcct cggtgtagcg ccgggcggcg gcgccgctgc
     5221 ccaccgtgat gatggcctgc accccggcgc cggcctgccc ggtgacggcg ctgaggtcga
     5281 agcgcaactg gcgcaccggc ttgccgccgt tggtgccctc gtcgatgcgg gccacctcgt
     5341 agtactcgat ggacgtttcc ttgaggttga cgcgcgcctg gtccgaggcc gtggcgagcg
     5401 agagcgtgcc gtcgtcgccc agcatgaccg ggctttgcgc cagcggcccg atgtgggcgc
     5461 catggtcgcg gtacatgtcc accgggtccc agttccagtt ccagagcgcg aactccttga
     5521 agcgcgcgtt ccagtcgggc atggcgtgaa gcgccttgat cggcgcctgg tgctccgatg
     5581 ctttgtagat ctgcccgatc agcgtggggt cgccgccggg gcggtgggtc aggtagaagg
     5641 gccagatata cgcgccgtac tgggccaggc cgtcatcctc gaacaggtcg agcggcccca
     5701 gcgggccgtc gttattgcgc gcccagccgc cgtcctgcaa ctggctctgc tcctggtcca
     5761 gcgtggggta gaccaggtcc tcggcccagg tcgccgtggc ctcgaggaac cacccgtcgt
     5821 cggcgacgtc gtaggcgaac tgggtcgcgt ggtagtactc gtgtgcggcg gtcacctgca
     5881 ggttctcgac cggcgtccgc gggaagccgg cgtagtcgtt gtcgaccacg cagaa
//
LOCUS       DASUYC010000052         4628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1946227, whole genome shotgun sequence.
ACCESSION   DASUYC010000052 DASUYC010000000
VERSION     DASUYC010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4628
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1946227"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="VF276_00840"
     CDS             complement(<1..753)
                     /locus_tag="VF276_00840"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX6605436.1"
                     /translation="MARARMGHMERQRAIQGWLFVSPFVVGFLLFFAGPMLFAAYISL
                     NRWPVLGEPTFIGLTNFTNMFADDLFWTSLRITTIYTIFSVPLGMIAGLALAMLLNTK
                     VKALALFRTLYYVPAVITGVALALLWWMMFNTEYGLINNALAIIGIPRIPWLTHTQWA
                     LPALILMSLWQVGGGMVIYLAGLQGIPSELYEAATVDGAGGWSKVRHITIPMLSPVLL
                     FNLVLGIIGSFQSFTNALIMTNGGPSQSTLVLV"
     gene            complement(814..2235)
                     /locus_tag="VF276_00845"
     CDS             complement(814..2235)
                     /locus_tag="VF276_00845"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6605437.1"
                     /translation="MLRTSKAGLALLLVLAVLLTACGESPATSTSVPAAATDTPAPAA
                     AATDTPATGGGEATATATIGIPTVGPTPVVPAGVTTIKVVDWVAENTSSWANRWYKPF
                     EEANPDIKIVHEQITGNYIETVLTRLTGSNDIDLIWVAPDSVGSFLRSGAALDLTDKI
                     KAEGDSLQLDKFPQWTKDDYTTVRYPDLKVGAGQYGLPLVMFVWEMWHNADMEAAAGL
                     STPQRGWTWDDFATMSKKMTDESKHQYGYQNQNWTLPLYTWVWQNGGDILTPDAKQMV
                     VGQPPAVEAFTFLQDKQVNDKIFVPIDQTTGANPIGFDSGNAGMITRGNWNLDLSRDW
                     KFKWDLSYPPKNKTEGTLGEELGLIINKSSPKADAAWKVLKWIAGPEGQRTLAVNDVV
                     PNTAVMSEVGLQAAPDNVRSLVVPLAGDAMVHSAPQWFRPKYTPGDFQDRLSVLWTGE
                     KKAADLLPAIEKEFNEALSKPLP"
     gene            complement(2312..3322)
                     /locus_tag="VF276_00850"
     CDS             complement(2312..3322)
                     /locus_tag="VF276_00850"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX6605438.1"
                     /translation="MLRRLALLLLLVLSVSACGGDQSYTAVTVDNAPSVARIKILTGH
                     TGGVNAVVWSPDGKMIASAGDDRAIYLWDPDTGKEIRHWSGHTLAITSLAFAPDGKTL
                     ASGGRDKAVILWDPADGRQLRTFSDSDQILALAFSPDGKLLASTGRSKTVIFSDPSTL
                     QPVRTIKGFGDRVNDLAFSPDGATLATAAGDNKVQTWDVASGAKKLEITDHKAGATAV
                     AFSPDGKMLATGDGAAKIRVWDAASGSAIATCAGLIGTVRDLAFSPDSKTLASASADH
                     AFALWNPATGARLGPGRTPAEQAATGHGSEVNSVAWRPDGTIVATGSYDQRVLLWAVQ
                     IQ"
     gene            complement(3395..4411)
                     /locus_tag="VF276_00855"
     CDS             complement(3395..4411)
                     /locus_tag="VF276_00855"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX6605439.1"
                     /translation="MKPPTVKEVAQQAGVSVATVSRVLNGSTAVDGELRQRVMAAAEQ
                     LRYRPNRLARNLRTKSTKTIAIVVPDIENPFFVSVTRGLEEVAFREGYTVLVCNTDDD
                     PQRESVYLQVLLDEAVAGIVVCATDERRSSAQVLKIMEQGVAMVGLDRRLENAPVDGV
                     LSDNFGGTRTAVSYLIEQGHRRIGLIAGPAKYAPARERRMGYEQALLDHGIAVEPALI
                     KVTNFKGPEAAVATEELFTAGDPPTALFLSSGNVALGSLRALHRLGLLVPADVSLIVF
                     DDLEWADAYNPPLTVVSQNTRQLGGLAGDLLLRRLRGSTEPPQERHLATQLIVRGSVT
                     RPRS"
BASE COUNT          735 a         1477 c         1599 g          817 t
ORIGIN      
        1 caccaggacc agggtggact ggctgggtcc gccgttggtc ataatcaggg cgttggtgaa
       61 actctggaac gagccgatga tgcccagcac caggttgaac agcagcaccg gcgacagcat
      121 cgggatggtg atatggcgga ccttggacca accgcccgcc ccgtccacgg tcgccgcctc
      181 gtacaactcg ctggggatgc cctgcaaccc ggccaggtag atcaccatgc cgccgcccac
      241 ctgccacagg ctcatgagga tcagcgcggg caaggcccac tgcgtgtgcg tgagccaggg
      301 gatgcgcggg atgccgatga tcgccagcgc gttgttgatc agcccgtact cggtgttgaa
      361 catcatccac cacagcaggg ccagggcgac gccggtgatc accgcgggca cgtagtagag
      421 cgtgcggaac agcgccagcg ccttgacctt ggtgttcagc aacatcgcca gggccagccc
      481 ggcgatcatg cccagcggca cgctgaagat cgtatagatg gtcgtgatgc gcaacgaggt
      541 ccagaacaga tcgtcggcga acatgttggt gaagttggtc aggccgatga aggtgggttc
      601 gcccagcacc ggccagcggt tgaggctgat gtatgccgcg aacaacatcg gcccggcgaa
      661 aaagagcagg aagccgacca cgaacggcga gacgaacagc cagccctgga tcgcccgctg
      721 gcgctccatg tggcccattc tggctctggc cataaggctc tcctttggtc ccgcggcgcg
      781 gggagtgccc cgcgccgcag aacgctagaa ctgctagggc agcggcttcg acagcgcctc
      841 gttgaactct ttttcgatgg ccggcagcag gtcggccgcc ttcttctccc ccgtccagag
      901 gacggagagg cggtcctgga agtcgcccgg cgtatacttc gggcggaacc actgcggggc
      961 cgagtggacc atggcgtcgc cggcaagcgg caccaccagg ctgcgcacgt tgtcgggcgc
     1021 ggcctgcagg cccacttcgc tcatgacggc cgtattgggc accacgtcgt tgacggccag
     1081 ggtgcgctgg ccctcggggc cggcgatcca cttcagcacc ttccaggcgg cgtcggcctt
     1141 ggggctgctc ttgttgatga tcaggcccaa ttcttcgccc agcgtgccct ccgtcttgtt
     1201 cttcggcgga tacgacaggt cccacttgaa cttccagtcg cggctcaggt ccaggttcca
     1261 gttgccgcgg gtgatcatgc cggcgttgcc gctgtcaaac ccgatcgggt tggcgccggt
     1321 ggtctgatcg atgggcacga agatcttgtc gttgacctgc ttgtcttgca agaaggtgaa
     1381 cgcctcgacc gcgggcggct ggccgacgac catctgcttg gcgtccggcg tgaggatgtc
     1441 gccgccgttc tgccagaccc aggtatagag cggcagcgtc cagttctggt tctggtagcc
     1501 gtactggtgc ttcgactcgt cggtcatctt cttgctcatc gtggcgaagt cgtcccaggt
     1561 ccagccacgc tgcggcgtgc tgaggccggc ggcggcttcc atatcggcgt tgtgccacat
     1621 ttcccagacg aacatcacca gcggcagccc atactggccg gcgccgacct tcaggtcggg
     1681 gtagcgcacc gtggtgtaat cgtccttggt ccactgcggg aacttgtcga gctggaggct
     1741 gtcaccctcg gccttgatct tgtcggtcag gtcgagcgcc gcgccggagc gcaggaagct
     1801 gccgacgctg tcgggcgcga cccagatcag gtcgatgtcg ttgctgccgg tcaggcgggt
     1861 gagcaccgtc tcgatatagt tgccggtgat ctgctcgtgg acaatcttga tgtcggggtt
     1921 ggcttcttca aagggcttgt accagcggtt ggcccaggac gaggtattct cggccaccca
     1981 gtccacgacc ttgatcgtgg tcacgccggc cgggacgacg ggcgtcggcc cgacggtcgg
     2041 gatgccaatc gtggcggtgg ccgtggcctc gccgccgccg gtggcagggg tgtcggtggc
     2101 cgccgccgcg ggcgcggggg tgtcggtggc cgccgccggc acggaggtgc tggtcgcggg
     2161 ggactcccca caggcggtca gcagcaccgc cagcacgagc agcagcgcca aaccggcctt
     2221 ggatgtacgt agcatgcgaa gtttcctcct atgaacgggt cgttaacata tcgggttcgc
     2281 aaacagaccg tgtgaagtat aggccacctc cttactgtat ttgcaccgcc cagagcagca
     2341 cgcgctggtc atagctcccg gtggcgacga tagtgccgtc ggggcgccag gccacactgt
     2401 tgacttcgct gccgtggccg gtggccgcct gctcagccgg cgtgcgtccg ggcccgagac
     2461 gcgcgccggt ggcagggttc cacagggcga aggcatgatc cgcgctggcc gaggccaggg
     2521 ttttgctgtc gggcgagaag gccaggtcgc ggacggtgcc gatcaggccg gcgcaggtcg
     2581 cgatcgcgct gccgctggcc gcgtcccaga cgcggatctt ggcggcgccg tcgccggtcg
     2641 ccagcatctt gccgtcgggc gagaaggcca cggcggtggc gccggcctta tgatcggtga
     2701 tctccagctt cttagcgccg ctggcgacat cccaggtctg caccttgttg tcgccggccg
     2761 cagtcgccag cgtggcgccg tccggcgaga aggccaggtc gttcacccgg tcgccgaacc
     2821 ccttgatggt gcggaccggc tgcaacgtgg agggatcgga gaagatgacg gtcttgctgc
     2881 ggcccgtgga ggcgagcagc ttgccgtcgg gcgagaaggc cagggccagg atctggtcgc
     2941 tgtcggagaa ggtgcgcagt tgccggccat cggcggggtc ccagaggatc acggccttgt
     3001 cgcgcccgcc gctggcgagc gtcttgccgt cgggcgcgaa ggccagcgag gtgatggcga
     3061 gcgtgtgccc cgaccagtgg cggatctcct tgcccgtgtc ggggtcccat agatagatgg
     3121 cgcggtcgtc gccggccgag gcgatcatct tgccgtcggg cgaccagacg acggcgttca
     3181 ccccgccggt gtggccggtg aggatcttga tgcgggccac gctgggcgcg ttgtccacgg
     3241 ttacggcggt gtaactctgg tctccgccgc aggccgagac gctcagaacc agcagcaaca
     3301 gcaatgcgag acggcgcagc atctggtttg ctccttgcag cgaccccacg ccgggcgctg
     3361 cgctagggcc ggtctgccgg cgcagacggg cgggctagga ccgcgggcgc gtaaccgacc
     3421 cgcggacgat caactgggtc gccaggtggc gctcctgcgg cggctcggtg ctgccgcgga
     3481 ggcggcgcag caagagatcg ccggccaggc cgcccaactg gcgggtgttc tgggatacca
     3541 cggtcagcgg cgggttgtag gcatcggccc attccaggtc gtcgaacacg atcagggaca
     3601 catcggcggg caccagcagg cccagccggt gcagggcgcg caggctgccg agggcgacgt
     3661 tgccgctgct caagaacagc gccgtgggcg ggtcgcccgc cgtgaagagt tcttcggtcg
     3721 ccacggcggc ctcgggacct ttgaagttcg tcaccttgat cagggccggt tcgacggcga
     3781 tgccgtggtc gagcagcgcc tgttcgtagc ccatgcgccg ctcgcgggcc ggggcgtatt
     3841 ttgccggccc ggcgatcagc ccgatgcgcc ggtggccctg ctcgatcagg taggagacgg
     3901 cggtgcgcgt gccgccgaag ttatccgaga gcacgccgtc gaccggggcg ttttccaggc
     3961 gccggtcgag gccgaccata gccacgccct gctccataat cttcagcacc tgggccgaac
     4021 tgcggcgctc atcggtcgcg cagacgacga tcccggccac ggcctcgtcc agcagcacct
     4081 gcagataaac gctctcacgc tgcgggtcgt cgtcggtgtt gcagaccagc acggtatagc
     4141 cttcgcggaa ggccacttcc tccaggcccc gcgtcaccga cacaaagaag gggttttcga
     4201 tatcgggcac gacgatggcg atcgtcttgg tggacttggt gcggagattg cgggcgaggc
     4261 ggttcgggcg gtagcgcagt tgctccgcgg ccgccatcac gcgctggcgc aattcgccgt
     4321 ccacggcggt gctgccgttc agcacccgcg acaccgtcgc cacggagacg cccgcctgtt
     4381 gcgccacttc cttgaccgtc ggtggtttca tagtcgaaaa ccttcctgag taatcaatta
     4441 cagagatgat aggatgttac gcaagattag acccaggcta agaccatccc aggcacacca
     4501 gacggactca acacgcgctc aagtcgcggc ccatcggggc cctggctgcc ttgcggtcta
     4561 tagtaatcga tttctcaggc cttgtcaata gggcaacact gttgtctttg gtatcggttt
     4621 ctccaccg
//
LOCUS       DASUYC010000053         2060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_319070,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000053 DASUYC010000000
VERSION     DASUYC010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319070"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..722
                     /locus_tag="VF276_00860"
                     /pseudo
     CDS             1..722
                     /locus_tag="VF276_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009015792.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1476..>2060
                     /locus_tag="VF276_00865"
     CDS             1476..>2060
                     /locus_tag="VF276_00865"
                     /inference="COORDINATES: protein motif:HMM:NF019155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEX6605440.1"
                     /translation="MELQPLGQLEPEIQALVRDAFVKHQESRWIIHVIDQLWTRHLTT
                     MEGLRQSIGLQAYAQKDPLVEYKRNAYELFDELKAEIRQLGTRVLSLRIEPAAAPAPP
                     PQQQPARREAAHKEPARAPQAAVAGMIGAGAAADGASQGNGAARGGNAPRPAQPQQLP
                     RGKRHGKGSPAAVGATAGNKTPGRNDPCPCGSGRK"
BASE COUNT          387 a          712 c          700 g          261 t
ORIGIN      
        1 tcaaacgacg cggcatcgac caccaggtat tgaacgcaaa gttccacgag cgcgaggccg
       61 ccatcgtcgc cgacgccggc cagaagggcg ccgtgacgat tgccaccaac atggcgggcc
      121 gcggtaccga catcatcctg ggctttggcg tggccgaact cggcggcctg catatcgtcg
      181 gcactgagcg ccacgagagc cggcgcatcg acaaccagtt gcgcgggcgc gccggtcgcc
      241 agggcgaccc cggctcgtcg cgcttctatg tctccatcga agacgagatc atgcgccggt
      301 ccggcgtcaa ccagaacatc gtcaactcgc gcgtgttcaa cgcgctctgg gaagaggata
      361 tgcccatcga gcataacctg atctccaaga gcgtggagac ggcgcagacc aagatggaag
      421 gctacaactt cgacctccgc aaacacctgg tcgaatacga cgacgtggtg aacacgcagc
      481 gcgacgtgat ctacaaggag cgccgctatg tgctgaccca gggcgagccg cggaccatcg
      541 tgctcgacat gctggccgaa acggtggacg acctggcgaa tcccaaccgg gtgggcgcca
      601 atcccgccgc gccgcccccc acgaccccgc gcaagaaggg ccagatcgac gccgaaagcc
      661 tgttcgcggc cctggccgaa accttcagcg aggacgtgac ccggcgcatg gcccatcacc
      721 tgctgcgcga ccatgtcgcg gcgctgatcg agacggggaa ccgcgaagcc gtcggcgacg
      781 aactggcgac gatcgcggcg gaggcggccg ccgggcggct cgacctggtg cagggcgcca
      841 tcgaccgcga cctgcgcgcc ttctaccatg tcgatgaccc gatgtacgag atggtgcagc
      901 aggacagctt cgactacaac aagctgctga agatcatggc ggccatcctg ccgctgcccg
      961 ccgacctcac ggcgcccgac ctggaaggca tgcagtacga cgaggccaag caatacttcg
     1021 gcgatctggc cgccgccgag ttcgaccgcc agttcgccga tcgcctggcc gaggtgcagc
     1081 acgcggcgcg ggtggccgcc gagcgcggcg acgcgcatac cctggccgtg caggccgggg
     1141 tcacggagga gttgagcagc gacgccgagc tttcggaggc cgagcgcgaa gccggggctt
     1201 tgctcgacct ggtggacagc gcgttcccgc tgagtcccgc gtcggcggcg gcccggctgc
     1261 ccatcgatcc gggcagcacc atgggcgccc aggaactgcc gcgcatcatc ggcggccagt
     1321 tggaacagat ctatgccgag gtgcaggagc gcctgggtca ggacttcatc ctgcaaaacg
     1381 cccacgtgcc gctgctgggt atggtcgacc acagtatccc cgccccggtg taccgcgaga
     1441 tcgaggatgt ggtcggcatg gagacgctgg aggcgatgga gttgcagccg ctgggccagt
     1501 tggagccgga gatccaggcg ctggtgcgcg atgccttcgt caagcaccag gagtcgcgct
     1561 ggatcatcca tgtcatcgac cagctctgga cgcgccacct gaccacgatg gagggcctgc
     1621 gccagtcgat cggcttgcag gcgtatgcgc agaaggaccc actggtcgag tacaagcgca
     1681 acgcctacga actgttcgat gagttgaagg ccgagatccg ccagctcggc acgcgcgtgc
     1741 tgagcctgcg catcgagccg gcggccgccc cggcgccgcc gccgcaacag cagcccgcgc
     1801 gcagggaagc cgcgcacaaa gaacccgcca gggcaccgca agccgcggtg gccggtatga
     1861 tcggcgcggg cgccgccgcg gatggcgcga gccagggcaa cggcgccgcg cggggcggga
     1921 acgcaccgcg cccggcccag ccgcaacaac tgccgcgcgg caagcgccac ggtaaaggca
     1981 gccctgccgc ggtcggcgcc accgccggca acaaaacgcc gggccgcaac gacccctgcc
     2041 cctgcggcag cgggcgcaag
//
LOCUS       DASUYC010000054         3737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_98767,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000054 DASUYC010000000
VERSION     DASUYC010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98767"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="VF276_00870"
     CDS             <1..734
                     /locus_tag="VF276_00870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HEX6605441.1"
                     /translation="PIVACGGDGTIQAVAGTLLDLGSPVPLGIIAAGSGNDYAHGTLA
                     LPRELRPALAAALYGRPERVDAARLNDRWMINAFGTGIDANVAWDVRDMVESGRARVG
                     GTLLYTLSALKQVLFHYQRIPTLEITLNGRAWGTRRMLLLAVMLGPTAGGGYRLTPRA
                     SARDGQLDVLLARRMPQAKALFALPLAKAGRHGWLREVEMVHAREVAIRSARPVQAHM
                     DGELISDRSFDIRIMPGALTVMVPR"
     gene            complement(785..1972)
                     /gene="chrA"
                     /locus_tag="VF276_00875"
     CDS             complement(785..1972)
                     /gene="chrA"
                     /locus_tag="VF276_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEX6605442.1"
                     /translation="MKETIEPATAETGGNGGLGEVAALALRLGFTAFGGPAVHIAMLH
                     DEVVTRRRWVTDGYFLDLLGATNLIPGPNSTEMVMHIGHVRAGRRGLVVAGACFILPA
                     ACIVLALAWLYGQYGTTPAAAWLLYGIKPVIIAVVLQALWGLSKTAIKSRFLAVVGAA
                     TFILYLLGVNELVLLLGGGLLVMLAENAARLRAGAPPLAALLPFLGFAPRGWAALPAD
                     TPANLAQLFLTFLKIGAVLYGSGYVLLAFLRNDFVNRLGWLTDQQLLDAIAIGQFTPG
                     PVFTTATFIGYVVGSWPGAVLATVGIFLPSFIFVAVVNPFVPRLRRSPWMGALLDGVN
                     VTALGLMAAVTWELARAAVVDGLTAVLALVAAGLLLRFKVNSTWLVLGGGLVSLAYHL
                     LGG"
     gene            2276..3652
                     /locus_tag="VF276_00880"
     CDS             2276..3652
                     /locus_tag="VF276_00880"
                     /inference="COORDINATES: protein motif:HMM:NF017019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HEX6605443.1"
                     /translation="MAGDRSLLQSLHQHTQYDHLIWSAGGHGNVTRSSLESGFALKIR
                     SITFGAGLHERQPRGTLLGTLGHAAAAATAAYKEAGYEVQTVRLATSPLPQLLATPGA
                     DPDPVRLAVELEAGCRDAGIGFCSLGAIPAGRSDASLGLIERIPEVIAATRDIFASVQ
                     VGGAAREDGQLKGYVNLKAARHTAAAIRAIAAGTPAGAGNLRFAALANCPPHIPFFPA
                     AYYLPDADSRPTLAIALEAADLAVDAFAAADSPEDAQVRLVGALERASEVISPIAAHV
                     AAEYRLRFAGIDLSLAPTPGEAISIGRAFELLTGGPFGGPATLSAAAFLTDCLRRVRV
                     PRTGFSGLMLPVLEDDVLAAQAGQTYDVDSLLLYSAVCGLGLDTVPLPGDTTEAQLAG
                     IIADMATLAVRLDKPLTARLLPIPGARPGDPVAWDFFSVRAGHVLAVKPRRATGLFAT
                     APGLAFSD"
BASE COUNT          599 a         1342 c         1258 g          538 t
ORIGIN      
        1 gccccatcgt cgcctgcggg ggcgacggca ccatccaggc cgtcgccggc accctgctgg
       61 acctcggaag cccggtgccg ctcggcatca tagcggcggg ttctgggaac gactatgccc
      121 acggcacgct ggcgctgccc cgcgagctgc gcccggcgct cgccgcagcg ctgtatggcc
      181 ggcccgagcg cgtggatgcc gcccggctca acgaccgctg gatgatcaac gccttcggca
      241 cgggcatcga cgccaacgtg gcctgggatg tgcgcgatat ggtggagtcg gggcgggcgc
      301 gcgtcggcgg caccctgctc tataccctct cggccctcaa gcaggtgctc ttccattacc
      361 agcggatccc cacgctggaa atcaccctga acggccgggc ctggggcacc cggcgcatgc
      421 tgttgctggc cgtcatgctc gggccgactg cgggcggcgg ctaccgcctg accccgcgcg
      481 ccagcgcccg cgacgggcag ttagacgtac tgctggcgcg gcgcatgccg caggccaagg
      541 ccctgttcgc cctgccgctg gccaaagccg ggcgccacgg ctggctgcgc gaggtggaga
      601 tggtccacgc ccgcgaggtc gccattcgca gcgcccgccc ggtgcaggcc cacatggacg
      661 gcgaactgat cagcgaccgc tcgttcgaca tccgcatcat gcccggcgcg ctgacggtga
      721 tggtgccgcg ctaacccggc ccggcccaga cgcgctgtag gggcgggttt gcaaccggcc
      781 cggcctaccc gccgagtagg tggtaggcca gcgagacgag accgccaccc agcaccagcc
      841 aggtcgagtt caccttgaag cgcaagagca gccccgccgc caccagggcc agcaccgcag
      901 tcaacccgtc gaccacggcc gcgcgggcca gttcccaggt gacggcggcc atcaggccga
      961 gcgccgtgac gttgacgccg tcgagcagcg cgcccatcca gggcgaacgg cgcaaccggg
     1021 gcacgaacgg gttcaccact gcgacgaaga tgaacgacgg caggaagatg cccaccgtgg
     1081 ccagcacagc gcccggccag cttcccacca cgtagccgat gaacgtggcc gtggtgaaca
     1141 ccgggccggg tgtgaactgg ccgatggcga tcgcatcgag cagttgctga tcggtgagcc
     1201 agcccaggcg gttcacgaag tcgttgcgca agaacgccag cagtacatag ccgctgccat
     1261 agagcaccgc gccgatcttg aggaatgtca ggaaaagctg cgccaggttg gccggggtgt
     1321 cggcgggcag ggccgcccag ccgcgcggcg cgaatccgag gaacggcagc agcgcggcca
     1381 gcggcggcgc tccggcgcgc aaccgggcgg cattctcggc cagcatcacc agcaggcccc
     1441 cgcccagcag caagacgagt tcgttcactc ccagcaggta gaggataaac gtcgccgccc
     1501 cgaccacggc caggaaacgg ctcttgatcg cggtcttgct cagaccccag agcgcctgca
     1561 acaccacggc gataatcacc ggcttgatgc cgtagagcag ccaggctgcc gccggggtcg
     1621 tgccgtactg cccatagagc caggccaggg ccagcacgat gcacgccgcg ggcaggataa
     1681 agcaagcccc ggccaccacc agcccgcgcc gcccggcccg cacgtggccg atgtgcatga
     1741 ccatctcggt cgagttgggg ccggggatca ggttcgtggc gccgagcagg tcgaggaagt
     1801 agccgtcggt gacccagcgg cggcgcgtca ccacctcgtc gtgcagcatg gcgatatgca
     1861 ccgccgggcc gccgaaggcc gtgaagccca ggcgcaaggc cagcgcggcc acctcgccca
     1921 gcccgccgtt gccgccggtc tcagccgtcg ccggctctat cgtctctttc acgccatctc
     1981 cttgagtccc tcatgcaggt tccggccgcg tctcgtgcgt agccgcgtga tttatcgcgc
     2041 cctgctccca ttgtgtgtag ccgcgcgatt tatcgcgccg tactcgtcgg tcgttagcgt
     2101 aggcaaagcc caacggccac ctggaacaga agcaaatgcc ggttcaactt accgattaga
     2161 gagcaactac actaccgata aacgagtacg gcgcgataaa tcgcgcggct accgccccaa
     2221 tccagctcca cacgtaaatc cgccgttttt gcagcccggt ccttgatatg gtatcatggc
     2281 gggcgatagg agtctactgc agtcgctgca ccagcacacg caatatgatc acctgatctg
     2341 gagcgcgggg gggcacggca acgtgacgcg ctctagcctg gaaagcgggt ttgcattgaa
     2401 aatccgatcc atcacctttg gggccgggct gcacgaacgg caaccgcgcg gcaccctgct
     2461 gggcaccctg gggcacgcgg cggcggcggc caccgcagcc tataaggagg ccgggtatga
     2521 ggtccagacg gtgcgcctgg cgacctcgcc gctgccgcag ttgctggcga cgcccggcgc
     2581 cgaccccgac ccggtgcggc tggccgtaga attggaagcg ggctgccgcg acgccggcat
     2641 tggcttctgc tcgctgggcg cgatccccgc cgggcggtcc gacgcctcgc tgggcctgat
     2701 cgagcgcatc ccggaggtca tcgccgccac gcgcgacatc ttcgccagcg tgcaggtcgg
     2761 cggcgccgcg cgggaagatg ggcaactgaa gggttatgtc aacttgaagg cggcgcggca
     2821 caccgccgcc gcgatccggg ccatcgccgc cggcacgccg gccggcgcgg gcaacctgcg
     2881 cttcgccgcg ctcgccaact gcccgccgca catccccttc ttccccgccg cctattacct
     2941 gcccgacgcc gacagccgac ccaccctggc gattgccctg gaagcggcgg acctggccgt
     3001 ggacgccttc gccgccgccg acagtccgga agatgcgcag gtccggctgg tcggggcgct
     3061 ggagcgcgcg agcgaggtca tcagcccgat tgccgcccac gtggccgccg agtatcggct
     3121 gcgttttgcc ggcatcgacc tgtcgctggc gcccacgccc ggcgaggcga tcagcattgg
     3181 ccgggccttc gagttgctga ccggcggccc cttcggcggg ccggcgacgc tcagcgcggc
     3241 ggcattcctc accgactgcc tgcgccgcgt gcgcgtgccg cgcaccggct tctccggact
     3301 gatgctgccc gtgctggagg acgatgtgct ggcggcccag gccggacaga cctacgatgt
     3361 ggacagcctg ctgctctatt cggcggtctg cggcctgggt ctcgacaccg tgccgctgcc
     3421 cggcgatacc accgaggcgc aactggcggg catcatcgcc gacatggcga ccctggccgt
     3481 gcggctcgac aagccgctca cggcccggct gctgccgatc cccggcgcgc gccccggcga
     3541 cccggtggcc tgggactttt tctcggtccg cgcgggccac gtgctggcgg tcaaaccccg
     3601 ccgcgccacg ggtctcttcg ccaccgcgcc gggcctggcg ttcagcgact gacacgacga
     3661 gtggtcagtg gtcagtgatc agtggtcagt gatcagtagc tttcggtaag cggtacgggg
     3721 ttggtaaaca ccgaaat
//
LOCUS       DASUYC010000055         3219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1429846, whole genome shotgun sequence.
ACCESSION   DASUYC010000055 DASUYC010000000
VERSION     DASUYC010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429846"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="VF276_00885"
     CDS             <1..984
                     /locus_tag="VF276_00885"
                     /inference="COORDINATES: protein motif:HMM:NF025839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEX6605444.1"
                     /translation="DGKPRGLRVADIYTGSGFRFQVLLDRAMDIGAAEFNGKPLAWTH
                     PALGTPEQFDPHGYGWGRTFGGGLVTTCGLTHFGQPEQDGTQALGLHDRISHTHASHV
                     CVTEAWRGDDYVLEIEGQSRQAVLNGENLLLIRKISTRLGATTLTIDDTVRNDGYRST
                     PHMILYHCNFGFPVVSPDSVLLVDDEFANPRDAAARAGFDTHRQFDPPSPEYAEQVFF
                     HKPRVGAGGFVQAAIANRALGFGAYVRYRAAELPFLAEWKMMAMHDYVCALEPANQWE
                     TPRHKLREEGRLRFLAAGEEVHYHVELGVLPDAESIAAFEAALGQTPQTSG"
     gene            1218..2030
                     /locus_tag="VF276_00890"
     CDS             1218..2030
                     /locus_tag="VF276_00890"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX6605445.1"
                     /translation="MKSTNKLWVAMSGVAAIALLFGALLSTWLPQGAAAQSRPDSGKT
                     DPAPAATPAKPAQITVRGSGIVTAVPDTLVINIGVVMQDSTVAAAQTKAGAALDAMLA
                     KLKAANIDAKDYRTIQYNVEPVMDYSGAGKGPEAANQPPQLVGFRVTNILEVTVRDAA
                     KGPELIDSLVSAGANTIYGVTYTFGDRAAIEKQAYDSAMKDANDKAARLAGLAKQQLG
                     RIVSVSEASTGGGVPMPVDAKGGMGAGSAPIAPGQQTIGVDLIVTYEAQPSN"
     gene            complement(2027..2815)
                     /locus_tag="VF276_00895"
     CDS             complement(2027..2815)
                     /locus_tag="VF276_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605446.1"
                     /translation="MQMLTCGSIPPGDGPGVEDAINPDILSLYGPPFDLTSDMLFVAA
                     LAPAGPLRRAFPGVPFLAVAGRIPLLMWFSRIQEACFRDAAGKRRCLGGPESVLYNEL
                     NVIALLRRPAVFVPGIYATSPLTIRLGHRYGMPKQPTTMGLATTQRQIASRVIDGRRQ
                     SFVRARLLGSGRGVAALVSRFWPWWSWPARFPDGRQVRARIVATPRVQLARVRGGRLA
                     LDAAWLPRPVPLLPLGIYVPDQRMQLPSPDNLRWSGTEAPVSEA"
     gene            complement(2921..3037)
                     /locus_tag="VF276_00900"
                     /pseudo
     CDS             complement(2921..3037)
                     /locus_tag="VF276_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015190806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
BASE COUNT          564 a         1150 c         1062 g          443 t
ORIGIN      
        1 gacggcaagc cgcgcgggct acgcgtggcc gacatctaca ccggcagcgg cttccgcttc
       61 caggtcttgc tggatcgcgc catggacatc ggcgcggcgg agttcaacgg caaaccgctg
      121 gcctggaccc acccggcgct cggcacgccc gagcagttcg acccgcacgg ctacggctgg
      181 ggccgcacct tcggcggcgg gctggtgacg acctgcgggc tgacccactt cggccagccc
      241 gagcaagacg gcacccaggc cctgggccta cacgaccgca tctcgcacac ccacgcgagc
      301 cacgtctgcg ttaccgaagc ctggcgcggc gatgactatg tgctcgaaat cgagggccag
      361 tcccgccagg cggtgctgaa cggcgagaac ctgctgctta tccgcaagat ctccacccgg
      421 cttggcgcca ccaccctgac catcgacgac accgtgcgca acgacggcta ccggtcgacc
      481 ccgcacatga tcctctacca ttgcaacttc ggcttcccgg tggtcagccc ggactcggtg
      541 ctgctggtgg acgacgagtt cgccaacccg cgcgacgcgg cggcgcgcgc cggattcgac
      601 acgcaccggc aattcgaccc gccgagcccg gagtacgccg agcaggtctt ctttcacaag
      661 ccgcgcgtgg gcgccggcgg cttcgtgcag gcggccatcg ccaaccgcgc gctcggcttc
      721 ggcgcctatg tgcgctaccg ggccgccgaa ctgcccttcc tggccgagtg gaagatgatg
      781 gcgatgcacg actacgtctg cgcgctggag ccggccaacc agtgggagac cccgcgccac
      841 aagctgcgcg aggagggccg cctgcgcttc ctggccgcag gcgaagaggt tcactaccac
      901 gtggagctag gcgtgctgcc ggacgccgag tctatcgccg cgttcgaggc cgccctgggc
      961 cagactccac agaccagcgg ctgatcgccg cccggtacat acccgagcgc cgccccggct
     1021 ggataggccg ccgggcggcg tttttgtttg ccgccgtgac catggcacag gtgtcgatat
     1081 taccgttcgc cggatgcgcg aacgagtttt cgtgactttg caaccggcgg gaaccttcca
     1141 gcggggtggg gcgtctggat cagtgagggt ccaaagtcac attaaaacga gatggttgaa
     1201 cacaaggaga accagtgatg aagtccacta acaaattatg ggttgccatg agtggcgttg
     1261 ccgcgatcgc gttgctgttc ggcgccctcc tgtccacctg gctgccgcag ggcgccgccg
     1321 cccagagccg ccccgatagc ggcaagaccg atcccgcccc cgccgccacc ccggccaagc
     1381 cggcccagat caccgtgcgc ggcagcggca tcgtgaccgc cgttcccgat acgctggtga
     1441 tcaacatcgg cgtggtcatg caggacagca ccgtcgccgc cgcccagacc aaggcgggcg
     1501 cggccctcga cgcgatgctg gcgaagctca aggccgccaa tatcgacgcc aaggactacc
     1561 gcacgatcca gtacaacgtc gagccggtca tggattacag cggcgccggc aaggggccgg
     1621 aggccgccaa tcagccgccg cagttggtcg gcttccgcgt gaccaacatc ctggaagtca
     1681 cggtgcggga cgccgctaag gggccggaac tgatcgactc cctggtcagc gcgggcgcga
     1741 acaccatcta tggcgtcacc tacaccttcg gcgaccgcgc cgcgatcgag aagcaagcct
     1801 acgacagcgc catgaaggac gcgaacgaca aggccgcccg cctggcgggt ctcgcgaagc
     1861 agcagttggg ccgcatcgtc agcgtgagcg aagcctcgac cggcggcggt gtgccgatgc
     1921 cggtggacgc caagggcggc atgggcgccg gtagcgcgcc gattgctccc ggccagcaga
     1981 ccatcggcgt ggacctgatt gtcacctatg aggcgcagcc cagcaactaa gcctcgctaa
     2041 cgggcgcctc cgtgcccgac cagcgcagat tatccggcga cggaagctgc atacgctggt
     2101 cgggcacgta gattcccagg ggcagcaacg gcaccgggcg cggcaaccac gccgcgtcga
     2161 gggccagccg cccgccccgc acccgcgcaa gctgcacccg cggtgtcgcc acgatccggg
     2221 cgcgcacctg gcgcccgtcg gggaaccgcg ccggccagct ccaccacggc cagaatcgcg
     2281 agaccagcgc cgccaccccc cgccccgatc ccagcaaccg cgcccgcacg aaactctgcc
     2341 gccgcccgtc gatcacccgc gaggcgatct gccgctgcgt cgtcgccagg cccatcgtcg
     2401 tgggctgctt gggcatgccg tagcggtggc ccaggcggat agtcagcggg ctggtggcgt
     2461 agatgccggg gacgaacacc gccgggcggc gcagcagggc gatcacgttg agttcgttgt
     2521 agagcacgct ctccggcccg cccaggcagc ggcgtttccc ggcggcatcg cgaaaacacg
     2581 cctcttggat gcggctgaac cacatcagca gcgggatccg gcccgccacg gccagaaacg
     2641 gcacgcccgg aaaggcgcgg cgcagcggcc cggcgggcgc gagcgccgcg acgaacagca
     2701 tatcggatgt gaggtcgaac ggcgggccat agagcgagag aatatcgggg ttgatagcgt
     2761 cctccacgcc cggcccatcc cccggcggga tcgagccgca ggtcagcatc tgcatgggcg
     2821 gcctctagcg cccggcgcgc cggatgagcg ccaccaggcc gaacgtggcg gcggtccaca
     2881 gcagccgttt gcggttgggg tagaggtcca gcgcgaccag attgcgcgcg gctagcacca
     2941 ccgaggcgcc gcgctgcccg aactgcaaag ccgtctcccg gccaatgccg gaggacgcgc
     3001 ccgtgatgac gacaacctgc tcgttgatcg gtcgcatcgc gtttcccctc ctgttgattt
     3061 gccggggcct cgcggcagga cctcacgaca cggtccgtac tagcaagtct gtgaccacga
     3121 cccgcttcgc tgcgtgcgcc gggacgacaa atgtaggatg atcccgacag acggacgcca
     3181 cagcccggcc cgatcgtcta taatacagag gatcccggt
//
LOCUS       DASUYC010000056         6431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_561173,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000056 DASUYC010000000
VERSION     DASUYC010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6431
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561173"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VF276_00905"
     CDS             <1..684
                     /locus_tag="VF276_00905"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HEX6605447.1"
                     /translation="VRQTLRALRQVAPAARFIYVSSNAVFGGGRGLYRETERPDPAAR
                     HDPLRTYALTKAAGERAALAGWTNTIVARTSVVDGYDAQGRLSARLAALVAELQAGRP
                     LGRFTDRYLSPTLVDNLVAALLEISAPTFLYRGILHLAGAERVTDYTYALALARRLGV
                     SPALVRPEPLAGHPTMAASARDASLDVTFTQRLLQTRLLDVEDQLARLFPRVSSGAEA
                     ETPGGWHGY"
     gene            784..1227
                     /locus_tag="VF276_00910"
     CDS             784..1227
                     /locus_tag="VF276_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009358962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HEX6605448.1"
                     /translation="MADRTAQATWKGTLTEGSGTINSVGSGAISNLPVTWKARTESSD
                     GMTSPEELIAAAHAACYAMAFSNVLAKAGHPADQLNVTAVCSFEPKPEGGWKVSKMTI
                     DVEGRVPGVNDAEFKRLAQEGEAGCPVSGALRNNVDIELNAHLAA"
     gene            1405..2649
                     /locus_tag="VF276_00915"
     CDS             1405..2649
                     /locus_tag="VF276_00915"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEX6605449.1"
                     /translation="MTAPVRTLTPTLARRLAITRQRLAGPPPARDAAGILDVVRHLGC
                     LQLDPIAVVARSHQLVLWSRLGAYDLDDLDTLLWRERKLFEYWAHCASIVLTEDYPIH
                     QVQMRKSRAGSSNWATRVRDWIAANQELHDYILEELCTKGPRMSNQFEDQAEEHWESS
                     GWTAGRNVSRMLDFLWIRGQITVAGRAGIQKVWDLTERHLPDAVTLPELPEQEAVRVA
                     VQKSLRALGVGRRADINVHYTRGNYPRLPAVLAELEAEGRIAQVAVRDGDRAWPGPWY
                     IHVDDLPLLDRLAAGDWTPRTTLLSPFDNLICDRARTELLFDFNFRIEIYVPQAKRQY
                     GYYVLPILHGDRLIGRIDPRMDRKQGRLTVNAVYAEPDAPMTDETARAVAGAVGDLAA
                     FLGARDIAYTERVPDGWRHALN"
     gene            2880..4661
                     /locus_tag="VF276_00920"
     CDS             2880..4661
                     /locus_tag="VF276_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016362563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6605450.1"
                     /translation="MAHNLTIAAPARPQVPLQQQRALLARYLKPQRGKVALLAVLLFA
                     NIGLQLARPLIVRTFIDVALAQGPLDSLFNAGLLFLAVALVNQGVAVAETYVAEDVGW
                     TATNAMRADLVFHCLHLDMGFHNTRTPGELIERIDGDVTALANFFSRFVLLVAGNAVL
                     LAGVLVMLAREDWRVGAVMTLVALGALWVLARSRNIGVAATTEERQASAKLFGFLEER
                     LAGLDDLRANGGSAHTMRRYYEYMRDFFYKWRHAWMLRAIIILIAVALFVAIFILVLS
                     LGAALYQAGAVSLGTVYLFSAYVGMLQRPLNQITEQMQDLQKASASMLRVEELYHIER
                     QIQDGPGAGLPHGPLAVEFAGVTFGYGDETAIIDGVSFRLAPGEVLGLLGRTGSGKTT
                     LTRLLLRLYDPAAGIVRLGGRDVRDLPVEALRRAIGVVTQDVQLFRATVRDNLTLFDA
                     TIPDEHIVAVLRDLGLGAWFDALPAGLDTELEAGGRSLSAGEAQLLAFTRVFLRDPGL
                     VILDEASSRLDRATEQLIERAVDKLLAGRTGIIIAHRLGTVERADRILILDDGQVAEQ
                     GERARLAHDPGSRFHHLLRTGLEEVLA"
     gene            4658..>6431
                     /locus_tag="VF276_00925"
     CDS             4658..>6431
                     /locus_tag="VF276_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010500763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6605451.1"
                     /translation="MKTLRIVWRLALARPGYFALTLLTWSIFFSLPLVSGLLLRAFFD
                     ALSGHAPAGWTVWTAVALMVATELVRMTSFYSGFWVWFNLEGAIQTVLRKNMLAHILE
                     GPGARSLPDSPGEAISRFRDDVDEIVDYIDTYVDLTGTLIFAVVALGVMIQTDAAIAL
                     TAFVPLLCIIVFVNSLTGRIKRVRRRAREATGRVTGFLGEIFGAVQAVKVSSAEARVT
                     AHFRTINARRRKAALMDSLFTQLLDSFNMNTANLATGVVLILAAQAMRNGSFTVGDFA
                     LFVSYLEALMGLPRQIGRVLARHKQVSVSIDRMTALLQGAPSENLAASGPIYLDGPLP
                     SVPYAPKTGADHLRTLDVRGLTYRYPGSDRGIADIDLRLEQGHVTVITGRIGAGKTTL
                     LRALLGLLPAQAGQVRWNGQAVTDPATFFVPPRCAYTPQVPRLFSEPLRDNILLGLPA
                     ARVDLPGALDQAVLEPDVAEMEDGLDTVVGPRGVRLSGGQVQRSAAARMFVREPELLV
                     FDDLSSALDVETERLLWDRLFARGGATCLVVSHRRAALRRADHIIVLKDGRIEAEGRL
                     DHLLATSAEMQRLWHGEAEADTPAE"
BASE COUNT         1002 a         2253 c         2146 g         1030 t
ORIGIN      
        1 gtgcggcaaa ccctgcgcgc gctacggcag gtcgccccgg cggcgcggtt tatctacgtg
       61 tcgagcaacg ccgtgttcgg cggcgggcgc ggcctctacc gtgagaccga gcgcccggac
      121 ccggcagcac gccacgatcc gttgcggacc tacgcgctga cgaaagcggc cggtgagcgg
      181 gcggccctgg caggctggac gaatacgatc gtggcccgta cctcggtcgt ggacggctac
      241 gacgcccagg gccggctcag cgcgcggttg gccgccctgg tggcggagtt acaggcgggc
      301 cggccgctgg ggcgcttcac cgaccgctac ctctctccca ccctcgtgga taacctggtc
      361 gccgcgttgc tcgaaatcag cgcgcccacc ttcctctatc gcggcatcct gcacctcgcc
      421 ggtgcggagc gcgtgaccga ctacacctac gccctggccc tcgcccgccg gctgggggta
      481 tccccggcgc tggttcgccc cgaacccctg gccggccacc cgaccatggc cgcaagcgcc
      541 cgcgatgcca gcctcgacgt gacgtttacc cagcgcctct tgcagacgcg cctcctggat
      601 gtcgaagacc aactggcgcg gctgtttccg cgcgtctcat ccggcgccga agccgaaacc
      661 cccggcggat ggcacggata ttgatcccat ggaagcacca atgctaatag ttgcatatgg
      721 ttgatcggca gcgccatccc gagccgccga acgctttttt ggacaatgaa agtggaggat
      781 tacatggctg atcgaacagc gcaagcgaca tggaaaggta ctttgacgga gggcagcggc
      841 acgatcaatt ccgtgggcag cggggcgatt agcaatctgc ccgtgacctg gaaggcgcgc
      901 accgaaagct cggacggcat gaccagtccc gaggaactga tcgcggcggc ccatgcggcc
      961 tgctacgcga tggcgttttc caacgtgctg gcgaaggcgg gccacccggc ggatcagctt
     1021 aacgtgaccg cggtttgctc gtttgaaccg aagcccgaag gcgggtggaa agtcagcaag
     1081 atgacgatcg acgtggaggg ccgcgtgccc ggcgtcaacg acgccgagtt caagcgcctg
     1141 gcgcaagaag gcgaagccgg gtgcccggtg tcgggcgcgc tgcgcaacaa cgtcgatatc
     1201 gaactcaacg cgcacctggc cgcctgaccc gcagccggag gcggggcaac ggggagcata
     1261 cgccgcgtgg cgtatgctcc cttgttgtcg cctacacctt tcgccgcaat ttccccggcc
     1321 cgtacttctc aaaccgcccc gcatctgcta tactggtagc tcaggtagaa cattcgtgcg
     1381 attccatccc caggaggtag taatttgacg gctcccgtgc gcacactgac ccccaccctc
     1441 gcccgccgcc tggcgatcac gcgccagcgc ctggccggcc ccccgcccgc gcgcgatgcc
     1501 gccggcatcc tcgacgtggt gcgtcatctc ggctgcctgc aactcgatcc gatcgcggtg
     1561 gtggcgcgca gccatcaact ggtgctctgg agccgcctcg gcgcctatga cctggacgat
     1621 ctcgacacgc tgctctggcg cgagcggaaa ctgttcgagt actgggcgca ctgcgcctcc
     1681 atcgtcctca ccgaggatta ccccatccac caggtccaga tgcgcaagtc gcgcgcggga
     1741 tccagtaact gggcgacgcg ggtgcgcgac tggatcgcgg cgaatcagga gctgcacgat
     1801 tatatcctgg aagaactctg caccaaaggc ccgcgcatga gcaaccagtt cgaggaccag
     1861 gccgaggagc actgggagtc ctccggatgg acggcggggc gcaacgtcag ccgcatgctc
     1921 gacttcctct ggatccgggg gcaaatcacg gtggccgggc gggcgggcat ccagaaggtg
     1981 tgggacctga ccgagcgcca cctgccggac gccgtcacgc tgccggagct accggagcag
     2041 gaagcagtcc gggtggctgt gcaaaaatcg ctacgggcgc tcggcgtggg ccggcgggcc
     2101 gacatcaacg tccactacac gcgcggcaac tatccgcgcc tgcctgccgt gctggccgag
     2161 ttggaagccg agggccgcat cgcccaggtc gcggtccgcg acggcgaccg ggcgtggccg
     2221 ggaccgtggt acatccatgt ggacgacctg cccctgctgg atcgcctggc ggcgggcgac
     2281 tggaccccgc gcaccaccct gctctcgccc ttcgacaacc tcatctgcga ccgggcgcgc
     2341 accgagttgc tgttcgactt caacttccgc atcgagatct acgtgccgca ggccaagcgc
     2401 cagtacggct actatgtgct gcccatcctg cacggcgacc ggctgatcgg gcgcatcgat
     2461 ccgcgcatgg accgcaaaca ggggcgcctg acggtcaacg ccgtctacgc cgaacccgac
     2521 gcgcccatga cggacgaaac ggcgcgcgcc gtggccgggg ccgtcggcga cctggcggca
     2581 ttcctgggcg ccagggacat cgcctacacc gagcgcgtcc ccgacggctg gcggcacgcc
     2641 ctcaactagc ggcaatgagc cttgtcactc tgagcgcagc gaagagtctc gtcccgtgag
     2701 ataccaggac gagatgcttc actgcgttca gcatgacagg tgagagacat ttccacctat
     2761 aggcggaccg ggataaggcc taccgactga tgcgaatggg cgggcccggc gggtagaatc
     2821 gggcaagggc cgtgcgcttg ggcacggcct gcgcttgacc gcaaggaaca cccgccgcca
     2881 tggcccataa tctgactatc gctgccccgg cccggcccca ggtgccgttg caacagcaac
     2941 gcgcattgct ggcccgctac ttgaagcccc aacggggcaa agtggcgctg ctggccgtgc
     3001 tgctcttcgc caacatcggg ctgcaactgg cccgcccgct gatcgtgcgt accttcatcg
     3061 acgtggccct ggcccaggga ccgctcgaca gcctgttcaa tgccgggttg ctcttcctgg
     3121 ccgtcgccct cgtcaaccag ggcgtcgccg tggccgaaac ctacgtagcc gaggatgtgg
     3181 gctggaccgc caccaacgcc atgcgcgccg acctggtgtt ccactgcctg cacctggaca
     3241 tgggcttcca taacacgcgc acgcccggcg aactcatcga gcgcatcgac ggcgacgtga
     3301 cggccctggc gaatttcttc tcccgcttcg tgctgttggt ggccggcaac gccgtgctgc
     3361 tggccggggt gctggtgatg ctggcccgcg aggattggcg cgtgggcgcg gtcatgacgc
     3421 tggtcgcgct gggcgcgctc tgggtgctgg cgcgcagccg caacatcggc gtggcggcca
     3481 ccacggagga gcgccaggcc agcgccaagc tgttcggctt cctcgaagag cggctcgcgg
     3541 ggctggacga cctgcgcgcc aacggcggca gcgcccacac catgcgccgc tattacgagt
     3601 acatgcgcga cttcttctac aagtggcgcc acgcctggat gctgcgcgcg atcatcatcc
     3661 tgatcgccgt cgccctgttc gtcgccatct tcatcctggt cctcagcctg ggcgcggcgc
     3721 tctatcaggc gggcgcggtc agcctgggca ccgtctacct cttctcggcc tatgtcggca
     3781 tgttgcaacg cccgctgaac cagatcaccg agcagatgca agatctgcaa aaggccagcg
     3841 ccagcatgct gcgcgtcgag gaactctacc atatcgagcg ccagatccag gacggccccg
     3901 gcgccggcct gccgcacggg ccgctggccg tcgagtttgc cggggtcacg ttcggctacg
     3961 gcgacgagac agcgatcatc gacggcgtgt ccttccgcct ggcgccgggc gaggtgctgg
     4021 ggttgctggg ccgcaccggc agcggcaaga cgaccctgac ccgcctgctc ttgcgcctgt
     4081 atgacccggc ggcgggcatc gtccgcctgg gcggccgcga cgtgcgcgac ttgcccgtcg
     4141 aggcgttgcg gcgggccatc ggcgtggtca cgcaggatgt gcaactcttc cgcgccaccg
     4201 tccgcgacaa cctgaccctg ttcgacgcga ccatccccga cgagcatatc gtcgccgtgt
     4261 tgcgcgacct gggcctgggg gcgtggttcg acgcgctgcc cgccggcctg gacaccgaac
     4321 tcgaagcggg cgggcgcagt ctgtcggcgg gcgaggcaca gttgctggcc ttcacccgcg
     4381 tgttcctgcg cgatccgggc ctggtgatcc tggacgaagc ctcttcgcgc ctggaccggg
     4441 ccaccgagca actgatcgag cgcgccgtgg acaaactcct ggcgggccgc accggcatca
     4501 ttatcgccca ccggctgggc acggtcgagc gcgccgaccg catcctcatc ctggacgacg
     4561 gccaggtcgc cgaacagggc gagcgggcgc ggctggccca tgatcccggc tcgcgcttcc
     4621 accacctgtt gcgcaccggc ctggaggagg tgctggcatg aaaacgctgc gcatcgtctg
     4681 gcggctggcc ctggcccggc ccggctactt cgcgctgacc ctgctgacct ggagcatctt
     4741 cttcagcctg ccgctggtct ccgggctgct gctgcgcgcc ttcttcgacg cgctgagcgg
     4801 ccacgcgccc gccgggtgga ccgtctggac ggcggtggcc ctgatggtcg ccaccgaact
     4861 cgtgcgtatg acttcgttct acagcggctt ctgggtctgg ttcaacctgg aaggcgccat
     4921 ccagacggtg ctgcgcaaaa acatgctggc ccatatcctg gaaggccccg gcgcccggtc
     4981 gctgcccgac tcgccgggcg aggccatcag ccgcttccgc gacgacgtgg acgagatcgt
     5041 cgactacatc gacacctacg tggatctcac cggcacgctg atcttcgccg tcgtggccct
     5101 gggcgtgatg atccagaccg acgccgccat cgcgctgacc gcgtttgtgc cgctgctctg
     5161 catcatcgtc ttcgtcaact cgctgacggg gcgcatcaag cgggtgcgcc gtcgcgcccg
     5221 cgaggccacg ggccgtgtca ccggcttcct gggcgagatc ttcggcgcgg tgcaggcagt
     5281 gaaggtcagc agtgccgagg cgcgggttac ggcgcacttc cgcaccatca acgcgcggcg
     5341 gcgcaaggcc gcgctcatgg acagcctgtt cacgcagttg ctcgactcgt tcaacatgaa
     5401 cacggccaac ctggcgaccg gcgtggtgtt gatcctcgcc gcccaggcca tgcgcaacgg
     5461 cagcttcacc gtgggcgact tcgcgctgtt cgtcagctac ctggaggcgc tgatggggct
     5521 gccacgccag atcgggcgcg tgctggcccg ccacaagcag gtcagtgtct ccatcgaccg
     5581 catgacggcg ctgctgcaag gcgcgcccag cgaaaatctg gcggcgtccg gcccgatcta
     5641 cctcgacggc ccgctgccct cggtgcccta tgcgccgaaa accggagcgg accacctgcg
     5701 cacgctcgac gtgcgcgggc tgacctaccg ctaccccggc agcgaccggg gcatcgcgga
     5761 catcgatctg cggctcgaac agggccatgt cacggtgatc accgggcgca tcggcgcggg
     5821 caagacgacg ctgctgcgcg cgctgctggg cctgctgccc gcgcaggcgg gccaggtgcg
     5881 ctggaacggc caggccgtga ccgacccggc gaccttcttc gtgccgccgc gctgcgccta
     5941 cacgccgcag gtgccgcgcc tgttcagcga accgctgcgc gacaacatct tgctgggcct
     6001 gcccgccgcg cgggtggatc tacccggcgc gctggaccag gcggtgctgg aacccgatgt
     6061 cgccgagatg gaggatgggc tggatacggt ggtcgggccg cgcggcgtgc ggttgtcggg
     6121 cgggcaggtg cagcggtcgg cggcggcgcg tatgttcgtg cgcgaaccgg agttgctggt
     6181 ttttgacgac ctgtccagcg cgctcgatgt cgagaccgag cgcctgctct gggatcggct
     6241 gttcgcacgg ggcggcgcca cctgcctggt ggtctcgcat cgccgcgccg ccctccgccg
     6301 cgccgaccac atcatcgtgc tgaaagatgg gcggatcgag gccgagggcc gcctggacca
     6361 cctgctcgcc acgtccgccg agatgcaacg gctctggcat ggcgaggccg aggcggacac
     6421 gcccgccgaa c
//
LOCUS       DASUYC010000057         2865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1636571, whole genome shotgun sequence.
ACCESSION   DASUYC010000057 DASUYC010000000
VERSION     DASUYC010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1636571"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="VF276_00930"
     CDS             complement(<1..809)
                     /locus_tag="VF276_00930"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX6605452.1"
                     /translation="MTVLFRKRQPLAGVGLLMLALALCLVPGAAAPPTGAAPAAQIRA
                     GVEALPPGTSIETVLTDLNAPVAMAFDPGGRLFYTERASGRVRLYANGTLQPNPVINF
                     DVSNCSERGLLGIAIDPDFTANHYIYVYYTQGPGCETTENKVARFVERDGVGSNPVVI
                     FTSPQTAGNHNGGNIHFGPDGKLYITIGESANPSNSQNMRVPNGKLHRINPDGSIPAD
                     NPSFGAGALGSIYAVGLRNSFDFTFDPVVRGRIFASENGPGCDDEMNRIERG"
     gene            complement(952..2025)
                     /locus_tag="VF276_00935"
     CDS             complement(952..2025)
                     /locus_tag="VF276_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEX6605453.1"
                     /translation="MATDNAFPIFDGHNDTLLDLALPKPGEERSFFTRSEYGHLDLPR
                     AREGGFGGGFFAVFVPSFPRAEDDELGIIFTEDGYEVPMAPPVDLIPAQEMALALVAS
                     LFRLEAASDGQMQVVRTADQLARCLDEGVLAAVLHFEGAEAIDPDFDALEVFYQAGLR
                     SLGPVWSRPNIFGHGVPFQFPRSPDTGPGLTDAGRDLVRACNRLRIMIDLSHLNEAGF
                     WDVAALSDAPLVATHSAAHALCPSTRNLTDRQLDAIRDTGGMVGLTFTVSDLREDGGN
                     DADTPLDTMVRHIQYLTERVGIDGVGFGSDFDGTTVPAEIGDVTGLPGLVAALRESGY
                     NDAELRKLTHENWLRVLRATWGR"
     gene            complement(2044..>2865)
                     /locus_tag="VF276_00940"
     CDS             complement(2044..>2865)
                     /locus_tag="VF276_00940"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX6605454.1"
                     /translation="RGAAPGEEENASMRLAVLSDIHGNPFALDAVLADVAAQGGVDGY
                     WVLGDLVAFGPDPAAVMERLAALPNARFVRGNTDRYLVRNEDPPGMRERAAQEPSFLE
                     ILLEFARSIAWTRGYVTANGHLDRLADLPLEQRRTLPDGTRMLGVHAAPGTDDGPGIH
                     PGLSDDELAALLAPADADLVFVGHTHVPVDRTVGGVRVINLGSVSNPATADLRACYVL
                     LDADESGYQARFRRVHYDREAAIAAVERLAHPGARHILRYLRGENRSRWADQAAG"
BASE COUNT          412 a          970 c         1046 g          437 t
ORIGIN      
        1 ccgcgctcga tgcggttcat ctcgtcatcg cagccggggc cgttttcgct ggcgaagatg
       61 cgcccgcgca ccaccgggtc gaaggtgaag tcgaagctgt tgcgcaggcc cacggcgtag
      121 atcgagccga gcgcccccgc gccgaagctc ggattgtcgg cggggatgct gccgtcgggg
      181 ttgatgcggt gcagcttgcc gttgggcacg cgcatgttct gggagttact cggattggcg
      241 ctctccccga tggtgatgta gagcttgccg tcgggaccga agtggatgtt gccgccgttg
      301 tggttgcccg ccgtctgcgg cgaggtgaag atcacgaccg ggttcgaccc cacgccgtcg
      361 cgctcgacga agcgggctac cttgttctcg gtcgtctcgc agccggggcc ttgcgtgtaa
      421 tagacgtaga tgtagtggtt ggccgtaaag tcggggtcga tggcgatgcc cagcagcccg
      481 cgctccgagc agttgctcac gtcgaagttg atcaccgggt tgggctggag tgtgccgttg
      541 gcgtacaggc gtacccggcc cgaggcgcgc tcggtgtaga acaagcgccc gccgggatcg
      601 aaggccatgg cgaccggcgc gttcaggtcg gtgagcaccg tctcgatgga tgtgcccggc
      661 ggcagcgcct ccaccccggc gcggatctgc gcggcgggcg ccgcgcccgt gggcggcgcg
      721 gcggcccccg gcacgaggca tagcgcgagt gccagcatga gcaggcccac acccgccagt
      781 ggctgccgtt tgcggaacag cacggtcatg ttcggcacct ccatacgcgg gccgactgga
      841 aaccggcccc tacatcgcaa tcggagtgcg ggccggtttg aaaccggccc ctacaacgcc
      901 tgtcctacct gtcgatcata ccgcggcggg cgggcgttgg ttccagggcg gctagcggcc
      961 ccaggtggcg cgcagcacgc gcagccagtt ctcgtgggtc agcttgcgga gctcggcgtc
     1021 gttgtagccg gattcgcgca gggcggcgac caggcccggc aggccggtca cgtcgccaat
     1081 ctcggcgggc acggtggtgc cgtcgaagtc cgagccgaag cccacgccgt cgatgcccac
     1141 gcgctcggtc aggtactgga tgtggcggac catcgtgtcc agcggcgtgt cggcatcgtt
     1201 gccgccgtcc tcgcgcaggt cgctgacggt gaaggtgagg ccgaccatgc caccggtgtc
     1261 gcggatggcg tcaagctggc ggtcggtcag gttgcgcgtg gacgggcaga gggcgtgggc
     1321 ggcggagtgc gtcgccacca gcggcgcgtc ggacagcgcg gccacgtccc agaagcccgc
     1381 ttcgttgagg tgcgacaggt cgatcatgat acgcaggcgg ttgcaggcgc gcacgaggtc
     1441 ccgcccggcg tcggtcaggc ccggcccggt gtcgggtgag cgggggaact ggaacggcac
     1501 accgtggccg aagatgttgg ggcggctcca gaccgggccg agcgaacgca gcccggcctg
     1561 ataaaacacc tccagggcgt cgaaatcggg gtcgatggcc tcggcgccct cgaagtgcag
     1621 caccgccgcc agcacgccct cgtccaggca gcgtgcgagt tgatccgccg tgcggaccac
     1681 ctgcatctgc ccgtccgagg ccgcctccag gcgaaacagg ctcgccacga gggccagggc
     1741 catctcctgc gccgggatca ggtcgaccgg cggggccatg ggcacctcgt agccgtcctc
     1801 ggtgaagatg atgcccagtt cgtcgtcttc cgcgcgtggg aaggagggca cgaacacggc
     1861 gaagaacccg ccgccgaagc cgccctcgcg ggcgcggggc aagtccaggt ggccgtactc
     1921 gctgcgcgtg aaaaagctgc gctcctcgcc cggcttgggc agcgccaggt ccagcagggt
     1981 gtcgttgtgc ccgtcgaaga tcgggaaggc gttgtctgtc gccacgctgg gttcctttcc
     2041 ggctcagccc gccgcctggt cggcccagcg ggatcggttt tcgccgcgca gatagcgcaa
     2101 gatgtgccgc gcgcccggat gcgcgagccg ctcgaccgcg gcgatggccg cctcgcggtc
     2161 atagtgcacc cgccggaagc gggcctggta gccggactcg tcggcgtcca gcagcacgta
     2221 gcaggcccgc aggtcggcgg tggccgggtt gctgacgctg cccaggttga tcacgcgcac
     2281 gccgcccacc gtgcggtcca ccggcacatg ggtatggccg acgaagacca gatcggcgtc
     2341 cgccggggcc agcagcgcag ccagttcgtc gtcgctgagg ccggggtgga tgcccggccc
     2401 gtcgtcggtg cccggcgcgg cgtggacgcc aagcatccgg gtgccgtcgg gcagggtccg
     2461 gcgctgctcc agcggcaagt ccgccagccg atccaggtgc ccgttcgccg tgacatagcc
     2521 gcgcgtccag gcgatgctgc gcgcgaactc cagcaggatt tcgaggaagg acggttcctg
     2581 cgcggcccgt tcgcgcatac cgggcgggtc ctcgttgcgc accaggtagc ggtcggtgtt
     2641 gccgcgcacg aagcgggcgt tgggcagggc cgccagccgc tccatcaccg ccgccgggtc
     2701 gggcccgaac gccaccaggt cgcccagcac ccagtagccg tccacgccgc cctgcgcggc
     2761 cacatcggcc agcacggcat ccagcgcgaa cgggttgcca tgaatatcgg acagcaccgc
     2821 taaccgcatg cttgcattct cctcttcgcc cggcgccgcg ccccg
//
LOCUS       DASUYC010000058         2088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1055945, whole genome shotgun sequence.
ACCESSION   DASUYC010000058 DASUYC010000000
VERSION     DASUYC010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055945"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1365)
                     /locus_tag="VF276_00945"
     CDS             complement(<1..1365)
                     /locus_tag="VF276_00945"
                     /inference="COORDINATES: protein motif:HMM:NF044618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605455.1"
                     /translation="MAVLLAVVGFNLSNTRAAPAAPPAPRADGKLPQRVAVNVAAPGK
                     APAAPLDVLYDQMGSPSGTGTSSQEFEAAFSTYNDELADDFMVPAGVDWTVTGVDAAG
                     SYFNGVGPAVSFNVVFYNSTTSGGNTVPGSVVATRTGQSYTEGGGVETITLSTPVTLT
                     AGGHYWVSVQARMDFSAGGQWAWTDSSGATSNSPATWRNPGGGFGETNCVDWNLRTTC
                     VAGSTGTDQSFRINGTSGPSFTATATVTGTPPTATNTPTATATACVVPATLLTEDFET
                     GLGQFASAVATCAPGGCGWNAVTSDKHGGLQSAFAPDLTGISDQMLTLTSTVSIPANA
                     TSAVLSFWHRVALESTYDGGVLEFSTDGGSTWSTTVPTFITGGYNSTISTSFGSPIGG
                     RRAWSGTIGSTGNFVNVQVDLISYAGQSVSFRFREANDSSVAATGWWVDDVSVTVETP
                     CVGTP"
BASE COUNT          288 a          672 c          770 g          358 t
ORIGIN      
        1 gggcgtgccg acgcaaggcg tctccaccgt cacggacacg tcatcgaccc accagccggt
       61 ggccgccacc gacgagtcgt tggcttcgcg gaagcggaag ctcacgctct gcccggcata
      121 gctgatcagg tcgacctgga cgttgacgaa gttgccggtc gagccgatgg tgccactcca
      181 ggcgcggcgc ccaccgatcg ggctgccgaa gctggtggag atggtggagt tatagccgcc
      241 ggtgatgaag gtcggcacgg tggtggacca ggtgctgccg ccgtcggtcg agaactccag
      301 cacgccgccg tcataggtgg actccagggc cacgcggtgc cagaaggaaa gcaccgcgct
      361 ggtggcgttg gccgggatgc tgacggtgct ggtcagggtc agcatctggt cggagatgcc
      421 ggtcaggtcg ggtgcgaagg cgctctgcag gccgccgtgc ttgtcggagg tgacggcgtt
      481 ccagccgcag ccgccgggcg cgcaggtggc gaccgccgag gcgaactggc ccagcccggt
      541 ctcaaagtct tcggtcagca gggtggcggg caccacgcag gcggtggccg tcgcggtggg
      601 cgtattggtc gccgtgggcg gggtcccggt cacggtcgcg gtggccgtga agctggggcc
      661 cgaagtgccg ttgatgcgga agctctggtc ggtgccggtg gagccggcca cgcaggtggt
      721 gcgcaggttc cagtccacgc agttggtctc gccgaagccg ccgccggggt tgcgccaggt
      781 ggccgggctg ttggaggtgg cgccgctgct gtcggtccag gcccactggc cgcccgcgct
      841 gaagtccatg cgcgcctgca cggagaccca gtagtggccg ccggcggtga gcgtgacggg
      901 ggtgctcagg gtgatagttt cgacgccgcc gccctcggtg tacgactgcc cggtgcgcgt
      961 ggcgaccacg ctgcccggca ccgtgttgcc gccgctggtg gtgctgttgt agaacacgac
     1021 gttgaaggag accgccgggc ccacgccgtt aaagtacgag ccggccgcgt cgaccccggt
     1081 caccgtccaa tccacgccgg cggggaccat gaaatcgtcg gccagttcgt cgttgtaggt
     1141 gctgaaggcc gcttcaaatt cctgcgaact ggtgcccgtg ccggaggggc tgcccatctg
     1201 gtcgtagagc acgtccaggg gcgccgcggg cgctttgccc ggcgcggcca cgttgaccgc
     1261 gacgcgctgg ggcagcttgc cgtcggcccg cggggcgggc ggggccgcgg gcgccgcgcg
     1321 tgtgttgctc aggttgaatc ccacgacagc cagtagtact gccactacga tcgttattcc
     1381 ccattgccag gagaatcgcc gcatgatgca ctccttgtcc tacagattcc atagcccagg
     1441 ttgcgagatg ggtcgatgca gaaccggcgc ccgccagtgt tgtgaacgcg ggccgtgggg
     1501 ccgcgcgggt gcctcctttc cttggtgtac tcccctcgcg cgcgcggtcg gtccgcacgc
     1561 ggggttgcga cggtccgggc gggcgtggac tctgccgtta acgcgcaacg cagggattat
     1621 accatgtcgt atcgttttgc gacagggggt accgggcggg aatcgcgaat cgcgggtggg
     1681 tcgcccagcg gtgggccgtg ggccgccgtg tacctgcggc atcgctgtgt gaacagggac
     1741 gggcgacggg tgggcagaac attgtgcaac ggttctttcg ttgcactgcg tgtctcaggg
     1801 gatcggagat gattggtggt tgactccggt tgaagtttgt gctatgattc gctctgtact
     1861 atgggcgtcc gtccacgcgt cccccctaga cacccggccc gcctcatgcg gccggctccc
     1921 cgcggccagg agctatgctc acccacccga tcggcaccgg gctgcccccc gaccgatacc
     1981 gcttgctgca gcgaatcgta tctgacgccg cgcccggcca gggccgcccg tcccccgccg
     2041 cgcgccgccc cgctccggtc gcatcctgcc ctgagaggag ggatcgct
//
LOCUS       DASUYC010000059         3166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1024599, whole genome shotgun sequence.
ACCESSION   DASUYC010000059 DASUYC010000000
VERSION     DASUYC010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1024599"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="VF276_00950"
     CDS             complement(<1..420)
                     /locus_tag="VF276_00950"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX6605456.1"
                     /translation="MIRLSALADEISPDLDTQLVVLRAEGIHHLELRGAWGTNVLDLS
                     DAQVAEIGRAVAAAGLRVSGIASPIGKVPIDAPAAAEEPRLARAIAVAQALGTARIRI
                     FSFYPAAGEESPPPRDAVLARLRALTAQAAAAGVTLLH"
     gene            complement(417..1451)
                     /locus_tag="VF276_00955"
     CDS             complement(417..1451)
                     /locus_tag="VF276_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX6605457.1"
                     /translation="MAKPLRFGLVGCGVISGTHAEAIAGLPDAELVAVADANRARAEQ
                     LAMKYGVPMYTDLQAMLDGEQLDAVTICTASGLHGAHACQVMRSGRHVLVEKPMDIRR
                     EALDEMLRVQQETGVKLAVISQHRFDAASQQVHDLIAAGAFGRLVLGNASVAWWRSQA
                     YYDSGDWRGTRALDGGVLMNQAIHSIDLLQWLMGPVRTVYAYADTLTHRMESEDVAVA
                     VLRFANGALGTIAATTGGYPGDLTRIEVCGNQGAAVIENDKLAYLRVAGEDTANAAAT
                     DAGAPAVTGGVTAPLPTASGHARQIADLVRAIREDGTPEIDGLAGRQPVDLILAIYES
                     ARTGQEVTLP"
     gene            1648..2337
                     /locus_tag="VF276_00960"
     CDS             1648..2337
                     /locus_tag="VF276_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009671703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX6605458.1"
                     /translation="MKTANLQKISRLPLRDHVYNMLQRAIIAGEIAPGGRVRDQDLAA
                     QLGVSRTPVREALQRLEDEGLVETVPGALTRVTPLDTRAAREAFPVVAALHALATRLG
                     VPCLSSAERAAMEAANARLAAALDSRAVPEAIAADDDFHGVLLQAAGNGEIAQALARL
                     MPKVRRLEFAQFGSLAGRRSVEQHAAILAACARGAAADAATLVEENWLTLGQLIVESL
                     ERPESPPAAGA"
     gene            2358..>3166
                     /locus_tag="VF276_00965"
     CDS             2358..>3166
                     /locus_tag="VF276_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003386818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX6605459.1"
                     /translation="MQPPNDPNAPFEVHDPAILAEARAVLDACASPAEMQEAVLGAVA
                     RNEEWRGRRCLNLLAPEAPTSPTVRGLLAAEVGTRAAEGHIGAVNRWFAGTRHIDEIE
                     ALCVELLKRVFRSRYADHRLVASMIGNMAVYTALTEPGDVIMSIPQPFGGHSSNRPDG
                     PAGVRGLKIVDIPYDPVELEVDLDLFRKAAPLVRPRLVALGASMTLFPFPLREMAEVV
                     AEWGGTIFFDGAHQLGLIAGGQFQDPLREGAAVITGSAGKTFSGPQSGVIV"
BASE COUNT          497 a         1143 c         1040 g          486 t
ORIGIN      
        1 atgcagcagg gtcacgcccg cggcagcggc ctgggccgtc agcgcccgca ggcgcgccag
       61 caccgcgtcg cggggcggcg gagattcctc gcccgccgcc gggtagaacg agaagatgcg
      121 gatgcgggcc gtgcccaggg cctgggccac cgcgatcgcc cgcgccagcc gcggctcctc
      181 ggcagcggcc ggagcgtcga tgggtacctt gccgatgggc gaggcgatgc ctgaaacccg
      241 taaccctgcg gccgccaccg cgcgcccgat ctccgccacc tgggcgtcgc tgaggtccag
      301 cacgttggtg ccccaggcgc cgcgcagttc caggtggtgg atgccttcgg cgcgcagcac
      361 aacaagctgc gtgtccaggt cgggcgagat ctcgtcggcg agcgcgctga gccggatcat
      421 ggcagcgtca cctcctgccc ggtgcgggcc gattcgtaga tggcgaggat gaggtccacc
      481 ggctgtcgcc cggcaagtcc atcgatctcc ggcgtgccgt cttcccggat cgcacggacc
      541 agatcggcaa tctgccgtgc gtggccgctc gcggtgggca gtggcgccgt cacacctccg
      601 gtaacggcgg gtgcacctgc atccgtggcg gcagcgtttg ccgtgtcctc accggccacg
      661 cgcaggtagg ccagcttgtc gttctcgatc accgccgccc cctggttgcc gcacacctcg
      721 atgcgcgtca ggtcgccggg gtagccgccg gtggtcgccg cgatagtgcc gagcgcgccg
      781 ttggcgaagc gcaacacggc cacggcgaca tcctcgctct ccatgcggtg ggtcagcgta
      841 tcggcgtagg cgtagacagt gcgcaccggg cccatgagcc attgcagcag gtcgatggaa
      901 tggatggcct ggttcatcaa gaccccgccg tccagtgccc gtgtgccccg ccagtcgccg
      961 ctgtcgtagt acgcctgcga gcgccaccag gccacgctcg cgttaccaag caccagccgc
     1021 ccgaaggcgc ccgccgcgat gagatcgtgt acctgctgtg aggccgcatc gaaccggtgc
     1081 tggctgatca cggccagctt cacccccgtc tcctgctgca cgcgcaacat ctcgtccagg
     1141 gcttcacgcc ggatatccat cggcttctcg accagcacgt ggcgcccgct acgcatcacc
     1201 tggcacgcgt gggcgccatg tagcccgctc gcagtgcaaa tagtgacagc gtccaattgc
     1261 tccccatcaa gcattgcctg caagtcggtg tacataggca ccccatactt catcgccaac
     1321 tgctcggcac gggcccgatt ggcatcggcc acggccacca gctcggcgtc cggcaacccg
     1381 gcgatggcct ccgcgtgcgt ccccgagatt acaccacacc cgaccaaccc gaaccgtaac
     1441 ggctttgcca tatgttattg ctcctttcag ctaacagtac cgctatctgc cagcaccttt
     1501 tgtaccattg accctcctgc ccaccggcca gcaaaatctc gtgttgtagg ggctggtttg
     1561 caaaccggcc cagcacctat cattgcgatc aatcagcact ccaagcaagg gcttgacagg
     1621 ccattctgta tacaatatat tggatacatg aaaaccgcaa atctgcagaa aatcagccgc
     1681 ctgccgctgc gcgaccatgt ctacaacatg ctgcagcgag ccatcatcgc cggggagatc
     1741 gcgccgggcg ggcgcgtgcg cgaccaggat ctggccgccc agcttggcgt cagccgcacg
     1801 cccgtacgcg aggccttgca gcggctcgaa gacgaagggc tggtcgagac ggtgcccggc
     1861 gcgctgacgc gcgtcacgcc gctggacacg cgggccgccc gcgaagcctt tcccgtggtg
     1921 gccgcgctgc acgcgctcgc cacccgcctg ggcgtgccgt gccttagctc ggcggagcgg
     1981 gccgccatgg aggccgccaa cgcccgcctc gccgccgcgc tcgacagcag agctgtgccc
     2041 gaggccattg ccgccgacga cgacttccac ggcgtgctgc tgcaggcagc aggcaacggc
     2101 gagatcgccc aggcgctcgc ccggctcatg cccaaggtgc ggcgcctgga gttcgcgcag
     2161 tttggctcgc tggcgggccg ccgctcggtc gagcagcatg ccgccatcct ggccgcctgt
     2221 gcgcggggcg ccgccgccga cgccgctacg ctggtcgagg agaactggct gacgctcggc
     2281 cagttgatcg tggaatcatt ggaacgcccg gagtctccac ccgctgcggg cgcttagcac
     2341 agcaaggaga acatgccatg caaccgccga acgatcccaa cgccccgttt gaagtccacg
     2401 atcccgcgat cctggccgag gcccgcgcgg tcctggacgc ctgcgcctcg ccggcggaga
     2461 tgcaggaggc cgtcctgggc gcggtggccc gcaacgagga gtggcgcggg cggcgctgcc
     2521 tcaacctgct cgcgccggaa gcgcccacca gccccacggt gcgcggcctg ctggcggccg
     2581 aagtgggcac gcgcgcggcg gagggccaca tcggggcggt gaaccgctgg ttcgccggca
     2641 cccggcacat cgacgagatc gaggcgctct gcgtcgagtt gctgaagcgt gtcttccggt
     2701 cccgctacgc cgaccaccgg ctcgtcgcca gcatgatcgg caacatggcc gtctacaccg
     2761 cgctaaccga gccgggcgac gtgatcatga gcatcccgca gccgttcggc ggccactcca
     2821 gcaaccgccc agacggcccc gcgggcgtgc gcggcctcaa gatcgtcgat attccctatg
     2881 acccggtgga actggaagtg gacctggacc tcttccgcaa agccgcgccg ctggtgcggc
     2941 cccggctggt cgcgctcggc gcctcgatga cgctcttccc cttccccctg cgcgaaatgg
     3001 ccgaggtcgt tgccgagtgg ggcgggacga tcttcttcga cggcgcacac caactagggc
     3061 tgatcgcggg cgggcagttc caggacccgc tgcgcgaggg ggcggcggtc atcaccggat
     3121 cggcgggcaa gacgttcagc ggcccgcaga gcggcgtcat cgtctg
//
LOCUS       DASUYC010000060         2160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1701945, whole genome shotgun sequence.
ACCESSION   DASUYC010000060 DASUYC010000000
VERSION     DASUYC010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1701945"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VF276_00970"
     CDS             <1..422
                     /locus_tag="VF276_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HEX6605460.1"
                     /translation="QTPVVIHRVIYGSLERFLGILIEHLAGAFPLWLSPVQAVVIPIS
                     NVKHLEYAEQVRAQLKAARLRVELDDSSERMGAKIRQAQLQKVPYMLVVGDKEREAGT
                     VSVRLRSGADLGALPVDALIARLQAEIVGRALTSAES"
     gene            complement(588..2120)
                     /locus_tag="VF276_00975"
     CDS             complement(588..2120)
                     /locus_tag="VF276_00975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX6605461.1"
                     /translation="MSRIPRPARPLPWRAVLLVGGLAVIVGLNLATLLETPPPFVDEG
                     IYGGYAWDWIHTGHPFGMPATDAFQRHDYYPWTAMVFYTLGMIVAGPHLVALRLVSLL
                     FGLLLLAIIYTIGVRLLGPRGGLVAAGILAFTPSFAYSSHLARVDIMAAVCGFGAIAL
                     YLSDPDARRVPRAALAGLLIGAALAMHPNAVIYAPVIFALYLLDYGRRVLRRPGLWGF
                     AGGLGAGVILFLASQILPWADPAAVLARLGTGAGRTPPMLRLDPAVWLESLAGTGVLL
                     VVGLTFWLFLLVAAVVGLGRARTAAARRLLVILGGLLLQSVLVFSIHPIHYAILVVPA
                     AALVIAALLDGWVARPWRPGLATYVRAALAGGLIVAAGLSAWTVIPRQDYAAYESALA
                     AIRAAVAPGGAVLGTTAYWFAVPDHPYYNWEQPGYYRYNHPGATFLDSLTAMHPDVVV
                     TDGVLAPSIVDDVQALPPDIRFLYIPRAELTNFLATQGQLVAARPIGALGTVRIYKIH
                     WP"
BASE COUNT          421 a          700 c          786 g          253 t
ORIGIN      
        1 agcaaacgcc ggtggtgatc caccgggtga tctacggctc gctggagcgc ttcctgggca
       61 tcctgatcga gcacctggcg ggggcgttcc cgctgtggct cagcccggtg caggccgtgg
      121 tcatcccgat ctccaacgtc aagcacctgg agtacgccga gcaggtgcgc gcccaactga
      181 aggcggcccg gctgcgcgtg gaactcgacg acagcagcga gcgcatgggg gccaaaatcc
      241 gccaggcgca gttgcagaag gtgccctaca tgctggtggt gggcgacaag gagcgcgagg
      301 caggcacggt tagcgtgcgg ctgcgcagcg gcgccgacct gggcgcgctg ccggtggacg
      361 cgctgatcgc ccgcctgcaa gcggaaatcg tcggccgtgc gctgaccagc gcggagagct
      421 aagcaaccgg tacaggtcgg acaagacaca ggcgcagccg cgcgttcacg gctgcgcctg
      481 tgctatttcg cggcgggatt tacgtggtgg ccggcgcggc gagagttccg cctcactggt
      541 tgggcgggcc gttggcgacc agggaccggg gagcgcgcag acaacactca gggccagtgg
      601 atcttgtaga tacgcacggt gcccagcgcg ccgatgggcc gggcggcgac gagttggccc
      661 tgggtagcta aaaagttcgt tagctcggcg cggggaatat acaggaagcg gatgtcgggc
      721 ggcagcgcct ggacatcgtc gacgatcgac ggggccagca cgccgtctgt caccaccacg
      781 tcgggatgca tggccgtgag actgtcgaga aacgtggcgc cggggtggtt gtagcgatag
      841 tagcccggtt gttcccagtt atagtaaggg tgatcaggca cggcgaacca gtaagcggtc
      901 gtgcccagca cggcgccgcc cggcgccacg gcggcccgga tcgcggccag cgccgactcg
      961 taagctgcgt agtcctggcg cgggatcacc gtccaggcgc tcaacccggc ggcgacgatc
     1021 agcccgccgg ccagggccgc ccggacatac gtcgccaagc cgggccgcca gggccgcgcc
     1081 acccacccgt cgagcaaggc ggcgatcacc agcgccgccg ccggcaccac caggatggcg
     1141 tagtggatgg ggtggatgct gaacacgagc acagattgca gcagcagacc acccaggatc
     1201 accagcaggc ggcgagcagc ggcggtgcgc gcccgtccca gcccgacgac ggcggcgacc
     1261 aggaggaaga gccagaaggt gaggccaacc accagcaaca cgcccgtgcc cgcaagcgat
     1321 tcgagccaga cggcggggtc caggcgcaac atgggcgggg tgcgcccggc cccggtgccc
     1381 agccgggcca gcacggctgc cgggtcggcc cagggcagga tctgggaggc caggaacagg
     1441 atcacgcccg cgcccagccc cccggcaaag ccccacaggc cgggccggcg cagcacccgg
     1501 cgcccgtagt cgagcaggta aagcgcgaaa atcacgggcg catagatgac ggcattgggg
     1561 tgcatcgcca gcgccgcgcc gatcaatagg ccggccaacg cggcccgcgg cacacggcgg
     1621 gcgtccggat cggaaagata gagcgcaatc gcgccgaacc cgcaaacggc cgccataata
     1681 tcgacccgcg ccaggtggct ggagtaggca aaagagggcg taaaggccag gatgcccgcc
     1741 gcgaccaggc ccccccgcgg gccgagcagg cgcacgccga tggtatagat gatcgccagc
     1801 agcaacaggc cgaacagcag gctcacaagg cgcagcgcga cgaggtgcgg gccggcgacg
     1861 atcataccga gcgtgtagaa gaccatcgcg gtccagggat aataatcatg gcgctggaaa
     1921 gcgtcggtcg ccggcatgcc gaagggatgg ccggtgtgga tccagtccca ggcatagccg
     1981 ccatagatcc cctcgtcgac aaagggaggc ggcgtttcca gtaacgtggc gagattcaac
     2041 cctactatga cggccaggcc acccaccagc agcaccgcgc gccagggcaa ggggcgcgcg
     2101 ggccggggga ttcgggacat agaacggatc tccatggggc gtatggctag cacaggcggc
//
LOCUS       DASUYC010000061         2704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_674581,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000061 DASUYC010000000
VERSION     DASUYC010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674581"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..510
                     /locus_tag="VF276_00980"
     CDS             130..510
                     /locus_tag="VF276_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX6605462.1"
                     /translation="MSHRPVLEAIDPQAVAAARESALPDDMIALVVRVFGALADPTRA
                     RILYALIGQSLCVRDLAILTGVSESAVSHQLRILRDRRLVKSQRDGNVIFYSLDDHHV
                     GRLFREAAYHADHVRTNLPDHPYP"
     gene            584..2512
                     /locus_tag="VF276_00985"
     CDS             584..2512
                     /locus_tag="VF276_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEX6605463.1"
                     /translation="MTNKAIAICVSCVRGPRTFAGRYRDFLLAPGTLFTLASFILLMA
                     AIIQDPAGLNEAAQAGSLLYFAAALAGSMYIWWSAIQGIRAGDFTADIPVSLATAAAI
                     AIGQYSAAAVVAVLLLVGGLLEEFVSARAGNALAALARLLPDQVTVRRDGHDRVVPIA
                     AVIPGDILLVRSGERVPVDGVVVLGSASINQAAITGESLPVEKQAGDEVYAGTLNELG
                     ALELRATKVGEQTTLGQIRRMVAEAQEQKAPIERILDRYARLYTPAAILLGLLVWWWS
                     GDILRAITILIVFCPCVMVLATPTALVASIGNAALRGSLVKKGATIEALSRVDAVAFD
                     KTGTLTYGAPRLVMTIPLNGVAPLEILRLAAVAEKFSEHPLGRAVVQAAAGAGMAIPD
                     PDDFQVLPGAGVRATLAGQPVLLGRPQLLSEHGVGMPVDLQTQANVFAAAGRTVVGVV
                     AGRAPVGLLVLEDTLRPEARGVVERLQKLGVRTVLITGDNQATAARLAEQVGISEIYA
                     DVLPQQKVAIVQRLQAEGRRVAFVGDGVNDGPALAVADVGVAMGLGGTDVAIETAEIA
                     LLADDLARLPHLLRLARQAMGAIRQNLVFSLGVLALAVGLAIPGILSPVTGALLHELS
                     SIPVIANSARLIAAGERR"
BASE COUNT          404 a          876 c          965 g          459 t
ORIGIN      
        1 cggcgcccat ggagcgcgac ctccagcaga caggatagag gaacgggccg ggtgcccgca
       61 atcttgattg ggtagcccga ttctggtaga ctaattgaac aggtatgcag ataactttgg
      121 ggaggtaatg tgagccaccg tccagtcctg gaggcaatcg acccgcaggc cgtcgcggcg
      181 gcgcgggaat cggctttgcc tgacgatatg attgcgcttg tggtcagagt ttttggcgca
      241 ctggccgacc ctacccgggc ccgcattctc tacgcgctga tcgggcagtc gctctgcgtg
      301 cgcgacctgg ctatcctgac tggtgtctcc gagtccgcgg tctcccacca gttgcgtatc
      361 ctgcgcgacc ggcgtctggt caagtcacaa cgggacggca acgtgatctt ttactccctg
      421 gacgaccatc acgtcggcag gctgttccgc gaggccgcct accacgccga tcatgtccgc
      481 accaaccttc ccgaccatcc ctacccgtga ggcgttgctc cgggcgacct tttttgccga
      541 tctatctgag cctctgaaca aacaaatgaa tagaaaccca gccatgacga acaaagccat
      601 cgcgatttgt gttagctgcg tacgcggccc gcggactttc gccggccgct accgcgattt
      661 cctgctggcc cccggcacgc tattcacgct cgccagcttc atcctgttga tggcggcgat
      721 catccaggac ccggccgggc tcaacgaggc cgcccaggcc ggcagcctgc tctatttcgc
      781 ggcggcgctg gccggctcga tgtatatctg gtggtcggcc attcagggca tccgtgcagg
      841 cgacttcacc gcggatatcc cggtctccct ggccacggcc gcggccatcg ctatcggcca
      901 gtactcggcc gccgccgtag tcgccgtgct actgctggtc ggcggcctgc tcgaagagtt
      961 cgtctcggcc cgcgcgggga acgcattggc ggcgctggcc cggctgctgc ccgatcaggt
     1021 gaccgtccgc cgggacggac atgaccgggt cgtacccatc gcggcagtga ttccgggcga
     1081 tatcctgctg gtgcgctccg gcgaacgcgt cccggtagac ggcgtggtgg tgctgggcag
     1141 cgcctcgatc aaccaggcgg cgatcaccgg cgagagcctg ccggtcgaga agcaagccgg
     1201 cgacgaggtt tacgccggga cgctcaacga gctgggggcg ctggagttgc gggcgacgaa
     1261 ggtcggcgag cagacgacgc tgggccagat ccgtcgcatg gtggcggaag cgcaagagca
     1321 aaaggcgccc atcgagcgca tcctcgaccg gtacgcccgg ctctacactc cggccgcgat
     1381 tctgctcggg ctgctggtct ggtggtggag cggcgatatc ctgcgcgcga tcacgatcct
     1441 gatcgtgttc tgcccctgtg tgatggtgct ggccacgcct acggccctgg tggcctccat
     1501 cgggaatgcc gcgctgcgcg gcagcctggt caagaagggc gccaccatcg aggcgttatc
     1561 gcgggtggac gccgtggcct tcgacaaaac cggcaccctg acctacggcg cgccccggct
     1621 ggtcatgacc attccgctca atggggtggc gccgctggag atcctgcggc tggcggccgt
     1681 cgccgagaag ttcagcgagc acccgctggg ccgcgcggtg gtgcaggccg ccgccggcgc
     1741 cggcatggcc atccccgacc cggatgactt ccaggtgctg ccgggggccg gggtgcgggc
     1801 gacgctggcg gggcagccgg tactgctagg gcgcccacag ctcttgagcg agcacggcgt
     1861 tgggatgccc gtggatctgc agacccaggc gaacgtcttc gccgcagccg gccggacggt
     1921 ggtcggggtc gtggcgggcc gggcgccggt gggcctgctg gtgctggagg atacgctgcg
     1981 ccccgaagcc cgcggcgtgg tggaacggct gcagaaactg ggcgtgcgca cggtgttgat
     2041 caccggcgat aaccaggcca ccgcggcacg gctggccgag caggtgggca tcagcgagat
     2101 atacgcggac gtattgccgc agcagaaggt cgcgatcgtc cagcgcctgc aggcggaggg
     2161 gcggcgggtg gcgtttgtgg gcgatggggt caacgatggg ccggccctgg ccgtggcgga
     2221 cgttggggtc gcgatggggc tgggcgggac ggacgtggct atcgaaaccg cggagatcgc
     2281 cctgctcgcg gacgacctgg cccggctgcc ccatctgctg aggttggcgc ggcaggcgat
     2341 gggcgccatc cggcagaacc tggtcttctc gctcggcgtg ctggccctcg cggtcgggct
     2401 ggcgatcccg ggcatcctgt cccccgtgac gggggcgttg ctgcacgaac tgtcgtcgat
     2461 cccggtgatc gccaactcgg cccggctgat cgcggccggg gaacgacgct agcgcggcgc
     2521 cgagcgggaa gatccgcgcg ggccggtgtg cccgtacggg aagacgggtc gacggaacca
     2581 ggtgcgcttg tagctaccgg catacctagg tccgtggacg gtacgactgg cccgcgccgg
     2641 gcggtctttt ccggagcatc ttcaggcagg gctcgaacct cacgccgtgg gacggatcgg
     2701 gctc
//
LOCUS       DASUYC010000062         2900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_777246,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000062 DASUYC010000000
VERSION     DASUYC010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777246"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VF276_00990"
     CDS             <1..265
                     /locus_tag="VF276_00990"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6605464.1"
                     /translation="DAYNAEADIERVCTHRGFRRRGFARAVILECLDRLRAMGLRGAY
                     ITGYSPAAVALYRAVGAAEESTSFVYAASDPVSDREPPTVPTP"
     gene            complement(645..1304)
                     /locus_tag="VF276_00995"
     CDS             complement(645..1304)
                     /locus_tag="VF276_00995"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEX6605465.1"
                     /translation="MKNRSYNQYCGLAYALDVVGERWTLLIVRELVAGPRRFKDLMEG
                     LPDISTNLLADRLRSLEQQGILRRRVLPPPAGSTVYELTDVGQGLETALLELGKWGSR
                     FVPPSAEGVTVLGLNSYALTLKTFFRPEQAQGLDETYELHIGREVLQVQIARGTIAVQ
                     QGTARKADAVFYTDIETYLALLLGQIAPDAAIAEGRIRVEGDPAALSRLLAVCGVRGG
                     H"
     gene            1473..>2900
                     /locus_tag="VF276_01000"
     CDS             1473..>2900
                     /locus_tag="VF276_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014984764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6605466.1"
                     /translation="MNTADETRALVSAERAPRATTREWVGLAVLTLACLLYSMDLTVL
                     HLAVPRLSEDLKPSTTQLLWIIDIYGFLVAGSLITMGTLGDHIGRRKLLLIGAAVFSI
                     TSVIAAFSVSAEMLIVSRALMGVAGATLAPSTLSLIFNMFRDPRQRSVAVGVWISAFS
                     AGGAIGPVLGGLLLEHFWWGSVFLMALPVMALLLVLGPRVLPEFSDPAAGRLDLRSAA
                     MSLVAMLGVIYGLKEIAQHGLELVPALSIVVGVGVGVAFVRRQLALADPMIDVRLFRI
                     PSFSAALATNVLGVFVVVGYFLFIAQYLQLVLGLAPFEAGVWSVPSAVGFIVSSNLAP
                     RVLHRLRPAFVISTCLALAAVGLSLLLLAGPSSGLTVLVTASVVIALALSPLFALTTE
                     LIVGSAPPERAGAASGIAETGSELGGALGIAILGSIGTAVYRSDLAGSLPAGIPPAAA
                     AAAQDTLGGAVGVAAHLPAPLAGALL"
BASE COUNT          409 a         1003 c          987 g          501 t
ORIGIN      
        1 cgacgcgtac aacgccgaag ccgacatcga gcgggtctgt acgcatcgcg gctttcgccg
       61 gcggggcttc gcccgcgccg tcatcctgga gtgcctggat cgcctgcggg caatggggct
      121 acgcggcgcc tatatcaccg ggtacagccc ggcggcggtg gcgctgtacc gagcggtcgg
      181 cgcggctgaa gagtccacct catttgtata cgccgcgagc gatcctgtgt ccgaccggga
      241 accgcccacg gtgcccacgc cataggcgcg tacccggtcg gggagcggat ggccatagac
      301 gccgccgccc ccgcgccacc tgttgccagc ggacgcgaag attggccgcc tgccggtggc
      361 cggcgctgtc ttggagcggg ggatagcggg gggcgtggcg gatcatcgcc aactggtccg
      421 gcgccagttc ggcgtcgggc agcgcgaagg cgagcaaccg gcccgtgggc gcctgcaccg
      481 ggtcctccca accggcaaag gccggcccgc tgcccgcacc gacccggcgc cgcaggcgcc
      541 cggcggcacg ccccgctcag ggcccgcgca agccgtcggt ctccagcacc cgttggaccg
      601 ccgccaggaa gggatcaatc cgcacgcccg gtgcgtgggt tccgtcaatg cccgccgcgc
      661 actccgcaca cagccagcag gcgactcagg gcggcagggt cgccttcgac gcggatgcgg
      721 ccctcggcta tggccgcgtc cggggcgatc tggcccagca ataacgccag ataggtctcg
      781 atatcggtgt aaaatacggc atccgccttg cgcgcggttc cctgctgcac cgcaatcgtc
      841 cccctggcga tctgcacctg caagacctcc cggccgatat gcagctcata agtctcatcc
      901 agcccctgcg cctgctcggg ccggaaaaag gtcttgaggg tcagcgcgta ggaattcagg
      961 cccagcacgg tgacgccttc ggctgacggc ggtacgaacc ggctacccca tttgcccaac
     1021 tccagcagcg ccgtttccaa cccctgcccc acgtcggtga gttcatagac cgtggacccg
     1081 gcgggcggcg gcagcacgcg ccggcgcagt atcccctgct gctccaggct gcgcaatcga
     1141 tccgctagca aattggtgct gatgtccggc agcccctcca tcagatcctt gaagcggcgc
     1201 ggcccggcga ccaactcgcg gacgatcaac agggtccacc gttcgccgac gacatccagg
     1261 gcataggcga gcccacaata ctggttgtag ctgcgattct tcatactcct tattatacaa
     1321 caaattctcc ctgcttgaaa gaggggaatt catacaatag attttgaagt aagggcttga
     1381 ttttgcttcc gcattctcct gccccgccgg gatagattct gtataccgga aactgagaca
     1441 ggtggggtac tggaacccgt aaaggaggag ctatgaacac cgctgatgaa acacgggcgc
     1501 tcgtgagcgc cgagcgcgcg ccccgcgcga cgactcgcga gtgggtcgga ctggccgtgc
     1561 tcaccctggc ttgcctgctc tactcgatgg acctgaccgt gctgcacctg gcggtgcccc
     1621 ggctgagcga ggatcttaag ccgagcacca cgcagttgtt gtggatcatc gacatctacg
     1681 gcttcctcgt cgccggctcg ctgatcacga tgggcacgct gggcgaccac atcgggcgcc
     1741 gcaagctgct gctgatcggg gccgccgtgt tcagcatcac ctcggtaatc gccgcgtttt
     1801 cggtcagcgc cgagatgctc atcgtgagcc gcgcgctcat gggggtggcc ggggccacgc
     1861 tggcgccgtc cacgctgtcc ctgatcttca acatgttccg cgacccgcgg cagcggtcgg
     1921 tcgccgtcgg cgtgtggatc tcggccttct cggcgggcgg cgccatcggc ccggtgctgg
     1981 gcggcctgct gctggagcac ttctggtggg ggtccgtgtt cctgatggcg ctgccggtga
     2041 tggcgctgct gctcgtgctg ggaccgcgcg tgctgcccga attcagcgat ccggcggcgg
     2101 ggcgcctgga cctgcgcagc gcggcgatgt cgctggtcgc catgctgggc gtgatctacg
     2161 gcctgaagga gatcgcgcag catggcctgg aactggtgcc ggcgctctcc atcgtcgtcg
     2221 gcgtgggcgt cggggtggcg tttgtgcgcc ggcaactggc cctggccgat ccgatgatcg
     2281 acgtgcgcct gttccgcata ccgtcgttca gcgcggcgct ggcgactaac gtgctgggcg
     2341 tgttcgtggt ggtgggctac ttcctgttta tcgcgcagta tctgcagctc gtgctcgggc
     2401 tggcgccgtt cgaggcgggc gtgtggtcgg tgccctcggc ggtcgggttc atcgtcagtt
     2461 ccaacctggc cccgcgcgtg ctgcaccgcc tgcgcccggc gttcgtgatc agcacctgcc
     2521 tggcactggc agccgtcggg ctgagcctgc tcttgctggc cggcccatcg tccggcctca
     2581 ccgtcctcgt gaccgcatcg gtcgtcatcg ccctggcgct ctcgccgctg ttcgcgctga
     2641 ccacggagct gatcgtcggc agcgcgccgc cggagcgggc gggcgcggcc tcgggcatcg
     2701 ccgaaaccgg ctcggaactg ggcggcgcac tgggcatcgc catcctgggc agcatcggca
     2761 cggcggtata ccgcagcgac ctggccggta gcctgccggc cggcatcccg cccgcggcgg
     2821 cggcggcagc gcaggacacg ctgggcggcg cggtcggcgt ggcggcccac ctgcccgccc
     2881 cgctggccgg cgcgctgctc
//
LOCUS       DASUYC010000063         3130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1931472, whole genome shotgun sequence.
ACCESSION   DASUYC010000063 DASUYC010000000
VERSION     DASUYC010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1931472"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..808)
                     /gene="istB"
                     /locus_tag="VF276_01005"
     CDS             complement(29..808)
                     /gene="istB"
                     /locus_tag="VF276_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEX6605467.1"
                     /translation="MELTHQLTPKLKHLRLSGILDTLEARTRAAVDGQWSYVEFLERL
                     LEDEVERRAQKQLALRVRRANMRSDKTLEEFDFSFNPSISRQAVLALASCDYVRAKRN
                     VLICGPTGVGKSHLATALAHEACRQGFDVLVVDTAKMLSHLNGGRADGTWEKRLAAYV
                     RPALLVLDDFGLKPVRAPGAEDLYDVINERYERGSIVLTSNRAPGEWPELFGDPLLSS
                     AGLDRLGDRAEVLVLTGTSYRARGRRSAGAGPASAPAVVQQ"
     gene            complement(799..2328)
                     /gene="istA"
                     /locus_tag="VF276_01010"
     CDS             complement(799..2328)
                     /gene="istA"
                     /locus_tag="VF276_01010"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEX6605468.1"
                     /translation="MDLRMLLLHLRDTPSDRAVARDTGLHRQTVSRYRAWATTHGLLT
                     DPLPTLEQIQTLLATTLPTTAPPQNVSSVAPYRDQVLTLRAQGVEVAALYQRLLEQGY
                     RGSYAAVYRFVRSLEPRTPDATVRVERTPGEEAQIDFGFIGRLQDPATGAWRKAWAFV
                     MTLAWSRYAFVTFVFDQTVPTWLRCHQLAFEFFGGVPGRLVIDNLKAGITQACWDDPQ
                     VQASYRECAEHYGFRIAPCRPATPEHKGKVESGVHYVQRNFFGGRAPSTLPDANRDVL
                     GWCRTTAGERTHGTTREAPRARFEAVEAARLRPLPATGYEYGIWKLAVVHRDCYITFA
                     NAYYSVPFRLVGQQVRVRGGTSEIRVYTPDYELVATHARAAQAGSRQTHLDHLPPYKL
                     PGLTLTREHAQAQAAAIGPATAAVVAALLTDPVLERLPLAGRLVRLGERVGTARLEAA
                     CRRALEFGDPSYRTVAQILAAELETAPPPPPPPPPPAGATFRRSASELLGHLFGGQLW
                     N"
     gene            complement(2738..>3130)
                     /locus_tag="VF276_01015"
     CDS             complement(2738..>3130)
                     /locus_tag="VF276_01015"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEX6605469.1"
                     /translation="AHEEAVRFEHALSPRMEQRIIKLLHNPTRCPHGNPIPGLALRAP
                     RNRMVLADAAPGDDLQIERISEEAEVDPQLLAFLQRNGLVPDALLHVDLVLKFNHTFT
                     VQVAGDPEPVVVGEEVARLIWVFPRAEA"
BASE COUNT          501 a         1009 c         1103 g          517 t
ORIGIN      
        1 gcgcatggtg ccacctcccg ttttcgccct attgttgcac aaccgcgggg gcgctcgccg
       61 gccccgctcc cgcgctacgc cggcctcgcg cccggtaact cgtgccggtc agcaccagca
      121 cttccgctcg gtcgcccagc cggtccagcc cggctgaact caagagggga tccccgaaca
      181 actcgggcca ttcccccggt gcgcggttgc tggtcagcac aatgctgccc cgctcatacc
      241 gctcgttaat cacatcgtag aggtcttccg cgcccggcgc gcgaaccggc ttgagcccga
      301 agtcgtccag caccagcagg gccgggcgca catacgccgc cagccgcttt tcccaggtgc
      361 cgtcggcccg tccgccgttg agatggctca gcatcttggc cgtgtccact accagcacgt
      421 caaacccctg ccggcacgct tcgtgggcca acgccgtcgc caggtgactt ttccccacac
      481 ccgtcggccc gcagatcaac acgttgcgct tggcgcgcac gtagtcgcaa ctggccaggg
      541 ccagcaccgc ctgccgcgag atactggggt tgaagctgaa gtcgaactcc tccagggttt
      601 tgtcactgcg catgttggcg cgccgcaccc gcagcgccaa ctgcttctgc gcccggcgtt
      661 cgacttcgtc ttccagcagc cgctccagga actcgacata gctccactgc ccgtccacgg
      721 ccgcgcgggt gcgcgcctcg agggtgtcca gaatgcccga caggcgcagg tgcttgagct
      781 tgggggtcag ttggtgggtc agttccatag ctggcctcca aacaggtgcc ccagcagttc
      841 gctggcgctg cgccggaagg tcgcgccggc cgggggcggc ggcggcgggg gtggcggcgg
      901 cgccgtctcc aactcggcgg ccaggatctg ggccaccgtg cggtagctgg ggtcgccgaa
      961 ctccagggcc cgccgacacg cggcttctag gcgggccgtt ccgacgcgct ctcccaagcg
     1021 caccaggcgc ccggcgaggg gcaagcgttc cagcactggg tcggttaaca acgccgccac
     1081 gacggccgcg gtggcggggc caatggcggc ggcttgggcc tgggcatgct cgcgcgtgag
     1141 agtcaagccg ggcagcttgt acggcggcaa gtggtccagg tgggtctggc gactgcccgc
     1201 ttgggcggcg cgggcatggg tcgccaccag ttcgtagtcg ggcgtgtaca cgcggatctc
     1261 gctggttccc ccgcgcaccc gcacttgctg gccgaccaac cggaagggca ccgagtagta
     1321 ggcattcgcg aacgtgatgt agcagtcgcg atgtacgacg gctagtttcc agatcccgta
     1381 ctcgtagccg gtggccggca aggggcgtaa gcgcgccgcc tcgaccgctt caaagcgcgc
     1441 ccgcggggct tcccgcgtgg tgccatgggt gcgctcgccg gcggtggtgc ggcaccagcc
     1501 caggacatcc cgattggcgt cggggagcgt actcggtgcg cgcccgccga agaagttgcg
     1561 ctggacgtag tggacgccgg attcgacctt gcccttgtgt tcgggggtcg ccgggcggca
     1621 gggcgcgatg cggaagccgt agtgttcggc gcactcgcgg taggacgcct ggacctgggg
     1681 gtcgtcccag caggcctggg tgatgccggc cttgaggttg tcaatgacca gccgccccgg
     1741 gacgccgccg aagaactcaa aggccagttg gtggcagcgc aaccaggtgg gcacggtctg
     1801 gtcgaagaca aaggtgacga aggcgtagcg gctccaggcc aaggtcatga caaaggccca
     1861 ggctttgcgc caggcgccgg tggccgggtc ctggaggcgg ccaatgaacc cgaaatcgat
     1921 ctgggcttcc tccccagggg tgcgctcgac ccgcacggtg gcgtcgggag tacggggttc
     1981 aagcgagcgc acgaagcggt agacagcggc gtaactgccg cggtagcctt gctccagcag
     2041 ccgctggtag agcgcggcga cctccacgcc ctgagcgcgc agggtcagca cctggtcgcg
     2101 gtagggggcg acggaggaga cattctgggg cggcgcggtg gtcggcaagg tggtggcgag
     2161 cagggtctgg atctgctcca gggtgggcag ggggtcggta agcaggccat gggtggtggc
     2221 ccaggcgcgg tagcggctga cggtctggcg atgcaggccg gtgtcgcgcg cgacagcccg
     2281 gtcgctgggg gtgtcgcgca aatgcaagag caacatgcgt agatccatga cagtgactct
     2341 cctattgggc atagctcctc ccgcttggtg ctaaaggccg aatgacttgg gaggaacatg
     2401 atacatccac gcggagagcc gggggtggtc cggtggtaca ccggggccgc caagctggta
     2461 cactttggcc gcaaagcggc tgaggcggtc ccgccttacc cccggtacac tttgggtgca
     2521 aaccgctggt ctattctaac ccgcaaagtg acaccgagaa cgcagagaat gagatgtgat
     2581 tgaaaaggag ttggcccgaa ctaccgctcg gtagcctgga atctcgcaca ggtggggccc
     2641 ccggttcgcg agaatagcat aaataccact tctattaatg atctttgcgt tctcaacgcc
     2701 tctgcggtga atctgcacgg atgccaggtt gcccggctca ggcttcggcg cgggggaaga
     2761 cccagatcag gcgggcgacc tcttcgccga ccactaccgg ctccgggtcg ccggccacct
     2821 gcacggtgaa ggtgtggttg aacttgagca cgaggtcgac atgcaacagg gcgtcgggca
     2881 ccaggccgtt gcgctgcaag aaggccagca actgcggatc gacttccgcc tcttccgaga
     2941 tgcgctcgat ctgcaagtcg tcgccgggcg cggcgtcggc cagcaccatg cggttgcggg
     3001 gggcgcgcag ggccagcccc ggaatgggat tgccgtgcgg gcagcgcgtc gggttatgca
     3061 gcagtttgat gatccgctgc tccatgcgcg ggctgagggc gtgctcgaag cgcaccgctt
     3121 cttcgtgcgc
//
LOCUS       DASUYC010000064         3157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1603677, whole genome shotgun sequence.
ACCESSION   DASUYC010000064 DASUYC010000000
VERSION     DASUYC010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1603677"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VF276_01020"
     CDS             complement(<1..269)
                     /locus_tag="VF276_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6605470.1"
                     /translation="MGRRQGPSEERRAAILQAAEAVFTEAGYTGASIREIARRAEVSS
                     ALLYWFFPNKAKLFAAVLLAKVDAQGVLEFPPAIFAIPPQEFLPRL"
     gene            complement(463..1101)
                     /locus_tag="VF276_01025"
     CDS             complement(463..1101)
                     /locus_tag="VF276_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX6605471.1"
                     /translation="MTTHTPLARDVLAAAETHLAGADPVLAQIITTTGPCTLSRQVDL
                     FGALAESIISQQISVKAADAIMSRFLSLLPAGQLDPAAILALPEESLRAAGLSRAKAV
                     YLRDLAAHVADGRLDLARLGQEPDEAVIAALLPVKGIGRWTAEMFLIFALGRLDVLPV
                     DDLGFRRAVQLRYNLPDLPVRTTLPALAEPWRPYRSIATWYLWRSLNNTPAL"
     gene            complement(1311..2924)
                     /locus_tag="VF276_01030"
     CDS             complement(1311..2924)
                     /locus_tag="VF276_01030"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HEX6605472.1"
                     /translation="MSNGKYQLHRQERFNGKHGGGLAVAEPAHTKTRLAAAPATEAPV
                     GRRPAPPRPAAPDNLETGAVASPLQATQNMRKSPLILIVAQTIIDMLALLSALVLAYK
                     LRFETDIAPDYTSPSVETYSLMLGTTLLCLLLAFNMSGLYRLSRGASRVDEFYKLTAA
                     ISMGTVGSIAVNSVLLGDRFIYSRLILVFGWFFCIIFAATGRLIYSVAVGYLRQRGVS
                     LQRVLVIGQGDAARTILDRMQARPELGYRILGIIEPPRLTEERAVWDTPVLGRVEDIE
                     RVAREYRADEVIVAMPGATPAELFDIVSNCQDANVSIKLYPDIVQLMIEPEVNLGLLG
                     GIPMYNVRDVALRGWNRVLKRAFDMVFSAAVLVVSSPLLLLIALIVKLDSPGPVFFIQ
                     ERVGLDGKIIHVIKFRTMRTDVSGPGWTVEGDPRRTRSGKWLRRFSLDELPQFINVLL
                     GEMSVVGPRPEQLYFVQQFSLTIPRYMRRHREKAGITGWAQVNGLRGDTSIEERTRYD
                     LDYVERWSLLFDLKIIARTVVQLLRGSAY"
     gene            complement(2951..>3157)
                     /locus_tag="VF276_01035"
     CDS             complement(2951..>3157)
                     /locus_tag="VF276_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012954920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX6605473.1"
                     /translation="HMKGASSRKRSTRSIREFYRAMHVFYVKHYADKYNSLINILVRL
                     GITLRGALALALNATRPAEQKRVS"
BASE COUNT          560 a         1010 c         1056 g          531 t
ORIGIN      
        1 agccgcggca agaactcctg cggcgggatg gcgaaaatcg ccggtggaaa ctccagcacg
       61 ccctgcgcgt ctaccttcgc cagcagcacc gcggcgaaca gcttggcctt gttgggaaag
      121 aaccagtaca gcagcgccga actgacctcc gcccgccgcg cgatctcgcg gatggacgca
      181 ccggtatagc ccgcctcggt aaacaccgcc tcggcggcct gcaaaatcgc cgcccgccgt
      241 tcctccgacg gcccctgtcg tctccccata tgcctccttt ggtttgctta atcaatcgat
      301 tgattaatta cagagccatt atagatcggg agtgtgtgtc tgtcaatagc ccttccggcc
      361 ctgcttgtca cgctgagcgc agcgaagagt ctcgtctcgt ccgggaaagc cgggatgctg
      421 cactccgttc agcatgacaa cgaggagtgc cgcctctgtt tcttacagcg cgggggtgtt
      481 gttgaggctg cgccagaggt accaggtggc gatggagcgg tacgggcgcc agggttcggc
      541 cagcgcgggg agggtggtgc gcacgggcag atcgggcagg ttgtagcgca gttggacggc
      601 ccggcggaag cccaggtcgt ccaccggcag cacgtcgagc cggcccaggg cgaagatcag
      661 gaacatctcc gccgtccagc gcccgatgcc cttcaccggc agcagcgcgg caatgaccgc
      721 ttcgtccggt tcctgcccca accgggcgag atccagccgc ccatcggcca catgcgcggc
      781 caggtcccgc agatacacgg ccttggcccg cgacagcccg gcagcgcgca gcgactcttc
      841 gggtagggcc aggatggccg cggggtccaa ctgcccggcg ggcagcaggc tgaggaagcg
      901 gctcatgatg gcgtctgcgg ccttgaccga gatctgctgg ctgataatcg actcggcgag
      961 cgcaccgaac agatcgacct gccgcgacag cgtacaaggg ccggtggtcg tgatgatctg
     1021 ggccagcacg gggtcggcgc cggccaggtg cgtctcggcg gcggccagca cgtcgcgcgc
     1081 caggggcgtg tgggtggtca tgcgggtctc acaaaaagcg aggcgcaccg gtcaggtccg
     1141 gctgccccgc tgtaggggcc ggttcacaac cggcccgtca tctacccggc aggcgggtgc
     1201 tgcaaatccg cgctgtaggg gccggtttca aaccggccca ccgtctatcc gccaagccag
     1261 tgatgcagat ccgcgctgta ggggccggtt tcaaaccggc ccgccgctgc ctagtacgcg
     1321 ctgccgcgca gcagttgcac cacggtgcgg gcgataatct tcaggtcgaa gagcagcgac
     1381 cagcgctcga catagtcgag gtcgtagcgc gtgcgctcct cgatggaggt atcgccgcgc
     1441 aggccgttga cctgcgccca gccggtgatg ccggccttct cgcgatggcg gcgcatatag
     1501 cgcgggatgg tcaggctgaa ctgctgcaca aagtagagtt gctccggacg cgggccgacc
     1561 acgctcatct cgcccagcag cacattgatg aactgcggga gttcgtcaag cgagaagcgg
     1621 cgcagccact tgccgctgcg tgtgcggcgc gggtcgcctt cgaccgtcca gcccggcccg
     1681 ctcacgtcgg tgcgcatcgt gcggaacttg atcacgtgga tgatcttgcc gtcgaggccc
     1741 acccgctcct ggatgaagaa gaccggcccc ggcgagtcga gcttgacgat gagcgcgatc
     1801 agcagcagca gcggcgagga gaccaccagc acggcggcgc tgaagaccat gtcgaacgcg
     1861 cgcttgagca cgcggttcca cccgcgcagc gccacgtcgc gcacattgta catcgggatg
     1921 ccgccgagca ggcccaggtt aacttcgggt tcgatcatca actggacaat atcgggatag
     1981 agcttgatcg agacgttggc gtcctggcaa ttggagacga tgtcgaacaa ctcggcgggg
     2041 gtggcgccgg gcatcgccac gatcacctcg tcggcgcggt actcgcgggc cacgcgctcg
     2101 atatcctcga cccggcccag caccggcgta tcccagacgg cgcgctcctc ggtcaggcgg
     2161 ggcggctcga tgatgcccag gatgcggtag cccagttccg ggcgcgcctg catgcggtcc
     2221 aggatcgtgc gggcggcgtc gccttggccg atgaccagca cgcgctgcag gctcaccccg
     2281 cgctggcgca ggtagcccac cgcgacgcta tagatcaacc ggcccgtggc ggcaaagata
     2341 atgcagaaaa accagccgaa gacgagaatc agccgggagt aaataaagcg gtcgcccagc
     2401 agcaccgagt tgacggcgat ggacccgacg gtgcccatgg agatcgccgc ggtcagcttg
     2461 tagaactcgt ccacgcgcga ggcgccgcgg ctcaagcggt agaggccact catgttgaag
     2521 gccagcagca ggcagagcag cgtggtgccg agcatgagcg agtaggtctc gacggacggc
     2581 gaggtgtaat ccggagcaat gtcggtctcg aaccgcagct tgtaggcaag cacgagcgcg
     2641 ctcagcagcg ccagcatatc gatgatcgtc tgcgcaacga tcaggatgag cgggctttta
     2701 cgcatattct gcgtcgcttg cagcggcgat gctacggcgc cggtctccag gttgtccggg
     2761 gcggcgggcc tgggtggggc ggggcgccgc ccgaccggcg cttccgtcgc cggggcggcg
     2821 gccagccgtg tcttcgtgtg tgccggttcg gcgacggcca atccgccgcc atgctttccg
     2881 ttgaaccgtt cctggcggtg caactggtat ttcccgttgc tcataattcc gatcctgctt
     2941 tatgcccgat ttagctaacg cgcttttgtt cggcgggccg cgtggcattg agcgccagcg
     3001 cgagcgctcc gcgcaaggta attcccagcc gcaccagtat attgatcagg ctattgtatt
     3061 tatccgcata atgtttcaca taaaagacat gcatggcgcg atagaactcg cggatcgacc
     3121 gtgtgctgcg cttgcggctg ctggcaccct tcatatg
//
LOCUS       DASUYC010000065         2900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_847492,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000065 DASUYC010000000
VERSION     DASUYC010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847492"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2462
                     /locus_tag="VF276_01040"
     CDS             <1..2462
                     /locus_tag="VF276_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX6605474.1"
                     /translation="DLHSQTERIAAALHEQGVGPGTRVIFWAPNSPWWVAAYFAVLRL
                     GAIVVPLDARNTPDFVDRVAAQTEPVLALLAAATAPGWDSRVPVRLLESFADLPAGPP
                     PPLPADLGPQSLAVIAFTSGTTGDPKGVMLTHGNILADAINTNKHVPEFPTYRLLSIL
                     PLSHMLEEVVGLLLPLLRGAGVTYTASRQSTVLFRAIQEQQITTILLVPQAVELLMNA
                     IEQEVRAQGKEALWRRMQRVAGYLPPRARRLLFRQVHQRLGGHIDFLMCGGARLDPAL
                     IHKWALLGVPVLQGYGTTEAAPVITIDSMEAHIPAAVGKPIPGAQIRLAPDGEILTRG
                     PNVTPGYWKNPTATAEAFEDGWYKTGDLGEIDEQGFLYIKGRKRDRIVLASGQKVYPD
                     DIEPILKAVSGVADATVVGMESPHGPVVHAVVIPRSPEVDIPAVIREANAVLGTYQQI
                     RGYSAWPDSDFPRNALGKVKKFQVAATLVRPEGVPARAEPVLATATTPAGKLMRITAE
                     VAHVDPATLTPAATLGEGAGLDSLMQIELLAGIERELGVHLDEANIGPTTTLGELLAM
                     IEHRPAAPRTADAVPAGSPPPATGTATAQAAIRQANKALWYHKAFRSFARTVFSAVYR
                     VRIVGRRNLPATPAILCINHLGYADLFVPLFYLPTKPLIHVVGEEHVKHVSAFRNWLI
                     DHMNIMVPVDRSKPLEALYTMVDVLRADGSLLMYPEGIVSHTEGALQPLQEGAAHAAI
                     LANVPLVPAGITGAADLWFRKPLTLRIGQAIDPAAFAGDLDTRRAAMTAELTSRMRAL
                     LPGEPPYAGRKLLKNWLTHLF"
     gene            2579..>2900
                     /locus_tag="VF276_01045"
     CDS             2579..>2900
                     /locus_tag="VF276_01045"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6605475.1"
                     /translation="MAIPAAQPVVAPGAPGLPQSEAPPAPVAGVAAAPFSWKTAVQYS
                     VANLGASVVYSLFNFAMPQYLDTYKLNPSLIGLLANERSFVGAFIQPIVGRLSDRTRT
                     RLGRR"
BASE COUNT          458 a         1056 c          945 g          441 t
ORIGIN      
        1 ccgatctgca ctcgcagacc gagcgtattg ccgcggcgct ccacgagcaa ggggtgggac
       61 ccggcacccg cgtgatcttc tgggcgccca acagcccctg gtgggtggcc gcctatttcg
      121 cggtcctgcg cctgggcgca atcgtggtgc cgctcgacgc ccgcaacacg cccgatttcg
      181 tcgaccgggt cgcagcgcag acggagccgg tgctggcgct gctggccgcg gcgacggcac
      241 ccggctggga tagccgggtg ccggtccggt tgctcgaatc gttcgcggac ttgcccgcgg
      301 gcccgccgcc cccgctgccc gccgatctcg gcccgcagag tctcgccgtc atcgcgttca
      361 cttcgggcac caccggcgac ccgaagggtg tgatgctgac ccacggcaat atcctggccg
      421 acgcgatcaa caccaacaag catgtgccgg agttcccgac ctatcgcctg ttgtcgatcc
      481 tgcccctgag ccatatgctg gaagaggtgg tcgggctgct gctgccgctg ctgcgcgggg
      541 ccggcgtcac ctacacagcc agccgccagt ccaccgtttt gttccgcgcg atccaggaac
      601 agcagatcac cacgatcctg ctcgtgccgc aagcggtgga actgctgatg aacgcgatcg
      661 agcaagaagt ccgggcgcag ggcaaagaag cgctgtggcg ccgcatgcag cgggtcgccg
      721 ggtacctgcc gccgcgcgcc cggcggttgt tgttccgcca ggtgcaccag cgcctgggcg
      781 gccatatcga cttcttgatg tgcggcgggg cgcgactcga tcccgccctg atccacaaat
      841 gggccctgct cggcgtgccc gtgctgcagg gctacggcac caccgaggcc gccccggtca
      901 tcaccatcga ctcgatggag gcgcacatcc cggcggcggt gggcaaaccc atccccggcg
      961 cccagatccg cctcgccccc gacggcgaaa tcctgacccg cgggccgaat gtgacccccg
     1021 gctactggaa gaaccccacc gccaccgccg aggcgttcga ggacggctgg tacaagacgg
     1081 gtgacctggg cgaaatcgac gaacagggct tcctgtatat caagggccgc aaacgcgatc
     1141 gcatcgtgct cgccagcggg caaaaggtct atcccgacga catcgagccg atcctgaaag
     1201 cggtctccgg cgtcgccgac gccacggtcg tcggcatgga gtcgccccac ggcccggtcg
     1261 tccacgccgt cgtcatcccg cgatcgcccg aggtggatat cccggcggtg atccgcgaag
     1321 ccaacgccgt cctcggcacc taccagcaga tccgcggcta cagcgcctgg ccggactccg
     1381 acttcccacg caacgcgctg ggcaaggtga agaaattcca ggtggcggct acgctggtcc
     1441 gccccgaggg ggtgccggcc cgcgccgagc cggttctcgc gaccgcgacc acgcccgccg
     1501 ggaagctgat gcgcattacc gccgaggtcg cgcacgtgga cccggccacc ctgacccccg
     1561 ccgccacgct cggcgaaggt gccgggctcg actcgctgat gcagatcgaa ttgctggcgg
     1621 gcatcgagcg cgaactcggc gtccacctgg acgaggcgaa tatcggcccg acgacgaccc
     1681 tgggcgaatt gctggcgatg atcgaacacc ggcccgccgc cccgcgcacg gcggacgccg
     1741 tccccgccgg cagcccaccg cccgccaccg ggaccgcaac cgcgcaggcg gcaatccggc
     1801 aggcgaacaa ggcgctgtgg tatcacaaag ccttccggtc gttcgcccgc accgtcttca
     1861 gcgctgtcta ccgcgtgcgc atcgtcgggc ggcgcaatct gcccgccacc ccggccatct
     1921 tgtgcatcaa ccacctgggc tacgccgacc tgtttgtgcc gctgttttac ctgccgacga
     1981 agccgctgat ccatgtcgtg ggcgaggaac acgtcaagca cgtgagcgcg ttccgcaact
     2041 ggttgatcga ccacatgaac atcatggtgc cggtggaccg cagcaaaccg ctggaggcgc
     2101 tgtataccat ggtagacgtg ttgcgggcgg acgggtcgct gctgatgtac ccggagggga
     2161 tcgtcagtca caccgagggc gcgctccaac cgctgcagga aggcgcggcc cacgccgcga
     2221 tcctggcgaa cgtgccgctg gtgccggcgg gcatcaccgg ggccgcggat ctctggttcc
     2281 gcaagccgct gacgctgcgc atcggtcagg cgatcgaccc cgcggcgttc gctggcgatc
     2341 tggacacgcg ccgcgcggcc atgaccgccg aactgaccag ccggatgcgg gcgctcctgc
     2401 cgggcgagcc accgtatgca gggcgcaagc tgctgaagaa ctggctgacg cacctgttct
     2461 aaaccgatcc ggggcagggg cccccggtat ccgccccctg gccgccgatc cggcgcaacg
     2521 tgcgtctcgt tcgcgccgga tcggcccggt tccttaactg caactggagg ttattcccat
     2581 ggcaattcct gctgcccaac cggtcgtcgc tcccggcgca ccggggctgc cgcagagtga
     2641 ggcgccgccc gccccggtgg ccggggtggc ggcggcccct ttctcctgga aaacggcggt
     2701 gcaatacagc gtcgccaacc tgggcgcctc ggtggtctat tcgctgttta acttcgcgat
     2761 gccgcagtac ctggacacat acaagctcaa cccctcgctc atcggcctcc tggccaacga
     2821 gcggtcgttt gtcggcgcgt ttatccagcc catcgtcggg cgcctgagcg accgcacgcg
     2881 cacccgcctg gggcggcgcc
//
LOCUS       DASUYC010000066         2291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1666029, whole genome shotgun sequence.
ACCESSION   DASUYC010000066 DASUYC010000000
VERSION     DASUYC010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1666029"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="VF276_01050"
     CDS             complement(<1..826)
                     /locus_tag="VF276_01050"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEX6605476.1"
                     /translation="MADLGILASYAHPDDEQGVTGTVALYAQQGIRTGLICATRGELG
                     EISDPALATPETLGAVREQEMRTAAAIAQIQHVWFLDYRDSGFVNSPRNQDPEAFANA
                     DPDEVIGRIVRIIRAFQPQVIVTFDPTGGYGHADHIAISRWTTDAFHAAGEATRYPEA
                     GPAWAPARLYYAAFTRSFSRRLNAFIRKHNPESSFAAIDLDQMGLPDEEITNVVDVSD
                     LVALKLDSVRAHATQWNPDSPFARLSAETRNQWRRNESFKLAAGPRLPPGADPGDLF"
     gene            complement(1028..>2291)
                     /locus_tag="VF276_01055"
     CDS             complement(1028..>2291)
                     /locus_tag="VF276_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX6605477.1"
                     /translation="GPGESVAAAIAWLEANGRGKGAVTYRLRDWLISRQRYWGTPIPI
                     VYCEKCGEVPVPEDQLPVELPEDVDFRPTGESPLKLHPTWRFTTCPQCGGPAERDTDT
                     MDTFVDSSWYQYRYLSPHYDEGPFDPQFRDWLPVTQYTGGIEHAVMHLLYTRFWTKAM
                     RDLGLVQFDEPMVRLFNQGIILGPDGLRMSKSRGNVVDPDDLVQNYGADAVRCFLMFI
                     GPWDQGGPWNTRGMEGITRWLGRVWAVATEPGRAGAAGNEAVATDLRRRLHQTIRDVG
                     EDTDNFRFNTAISAMMELTNALMKARDELAGSPAWDETVRTFLLLLAPYAPHLAEELW
                     HRRGYEGSVHQQAWPTWDPALAAEETIEVVIQLNGKVRDRLTVPADSDAKALEQAALT
                     SNRVQDLIGGKAVRRVIVVPGKLVNILV"
BASE COUNT          328 a          746 c          819 g          398 t
ORIGIN      
        1 caaacaggtc gcctggatcc gcgccaggcg ggaggcgcgg gcctgccgcc agcttgaacg
       61 actcgttgcg gcgccactgg tttcgcgttt ctgccgagag ccgcgcaaag gggctgtcgg
      121 ggttccactg cgtggcgtgg gcgcgcaccg agtccagttt gagcgccacc aggtcggaca
      181 cgtctaccac attcgtgatc tcttcgtcgg gcaggcccat ctggtcgaga tcgatagccg
      241 cgaacgagct ttcggggttg tgcttgcgga tgaaggcatt caaccggcgg ctgaagctgc
      301 gcgtaaacgc ggcatagtag agccgggcgg gtgcccaggc ggggcccgcc tccgggtagc
      361 gcgtggcctc gcctgcggca tggaaggcgt ccgtggtcca gcggctgatg gcgatgtggt
      421 ccgcgtggcc gtagccgccg gtcgggtcga aggtgacgat cacctgcggc tggaacgcgc
      481 ggatgatgcg cacgatacgg ccgattacct cgtcggggtc ggcgttggca aacgcctcgg
      541 ggtcctggtt gcgcgggctg ttgacaaagc ccgagtcgcg gtagtcgagg aaccagacgt
      601 gctggatctg cgcgatggcg gcggccgtgc gcatttcctg ctcgcgcacg gcacccaggg
      661 tttcgggcgt cgccagcgcg gggtcggaga tctcgccgag ttcgccgcgg gtggcgcaga
      721 tcaggccggt gcggatgccc tgttgcgcgt agagcgccac cgtgcccgtg accccttgtt
      781 cgtcgtcggg gtgggcgtac gatgccagta tgccgagatc ggccatgcga taggactcct
      841 ctgccgcctg gggaccgtgc ggccccaggc ggtgggccgg tttcaaaccg gcccctacaa
      901 ttgcgattcc gtagtgattc caccgggtgg caggccggtt tgaaaccggc ccctaccagg
      961 gtgatcttac cgggcggcag gccggtttga aaccggcccc taccagggtg atcttaccgg
     1021 gcggctgcta aactaggatg ttgaccagtt tccccggcac gacgatgacc cggcgcacgg
     1081 ctttgccgcc gatcaggtct tgcacgcggt tgctggtcag cgcggcctgc tccagcgcct
     1141 tggcgtcgga atccgccggc acggtcagcc tgtcgcgcac cttgccgttg agctggatga
     1201 ccacctcgat ggtctcctcg gcggccaggg ccgggtccca ggtgggccag gcttgctggt
     1261 gcacgctgcc ctcgtagccg cgccggtgcc agagttcctc ggccagatgg ggcgcgtagg
     1321 gcgccagcag cagcaggaac gtgcgcaccg tctcgtccca ggcggggctg cccgccagtt
     1381 cgtcgcgggc tttcatcaag gcattggtca gctccatcat cgccgagatg gccgtgttga
     1441 agcggaaatt gtcggtatcc tcgcccacgt cgcggatggt ctggtgcagg cggcggcgca
     1501 ggtcggtcgc cacggcctcg ttgcccgcgg cgcccgcccg ccccggctcg gtggcgacgg
     1561 cccagacgcg gcccagccac cgggtgatgc cctccatgcc gcgcgtgttc caggggccgc
     1621 cctggtccca tgggccgatg aacatcagga agcagcgcac cgcgtcggcg ccgtagttct
     1681 gcaccaggtc gtcgggatcg accacgttgc cccggctctt gctcatacgc aggccgtccg
     1741 gccccaggat gatgccctgg ttgaacaggc gcaccatcgg ctcgtcgaac tgcaccagcc
     1801 ccaggtcgcg catggccttg gtccagaagc gcgtgtagag caggtgcatg acggcgtgct
     1861 cgatgccgcc ggtgtactgc gtgaccggca gccagtcgcg gaactgcggg tcgaacggcc
     1921 cctcgtcgta gtgcgggctg aggtagcggt actggtacca cgaactatcg acaaacgtgt
     1981 ccatggtatc ggtgtcgcgc tcggcggggc cgccgcattg cgggcaggtg gtgaaccgcc
     2041 aggtggggtg cagcttgagc gggctttcgc cggtgggccg gaaatcgaca tcctcaggca
     2101 actctacggg caactggtct tcgggcacgg gcacctcgcc gcacttctcg cagtagacga
     2161 tagggatggg cgtgccccag tagcgctggc ggctgatcag ccagtcgcgc aggcggtagg
     2221 tcacggcgcc cttgccccgc ccgttggcct ccagccaggc aatagccgcc gccacgctct
     2281 cgccggggcc a
//
LOCUS       DASUYC010000067         5713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_331026,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000067 DASUYC010000000
VERSION     DASUYC010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5713
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331026"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /gene="dprA"
                     /locus_tag="VF276_01060"
     CDS             <1..759
                     /gene="dprA"
                     /locus_tag="VF276_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX6605478.1"
                     /translation="WAIGVVGTRQVTSYGREVAARLAGDLARSKVTVVSGLARGVDTL
                     AHTAALDADGRTVAVLGSGLDQLYPAENRTLARRIADGRGAVITDYPLGTRPEAINFP
                     PRNRIISGLSLGVLIVEAGPGSGALITHRFALEQGREVFAVPGSIFNVQSQGTNDLLK
                     QGAKCVTRVEDILEELKMGLVAEHVQAVLALPEDPTERTLLAWLQRDAEPQHIDAIVR
                     GCNLPAYVVSSTLTMMELKGLVRQVGALQYVPVR"
     gene            1273..2985
                     /locus_tag="VF276_01065"
     CDS             1273..2985
                     /locus_tag="VF276_01065"
                     /inference="COORDINATES: protein motif:HMM:NF010167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6605479.1"
                     /translation="MNEIVAQPPVIEVAELSFQYAAAGTRTLDNISFQVPAGSLLLVA
                     GPSGCGKSTLLRCLNGLIPHSYKGTLWGEARLGGEDIFHLSRAALARRVGTVLQDPSR
                     QLVASTVGSDVAFGPENLGLPVDEIEARVTRALGLLGLEGLRERETQALSGGEHQKVA
                     IAGVLALDPTVLLLDEPLANLDPRSAWEALQLFRRLADSGRSIILVEHRVEDALKIAP
                     EQVILMDSGRITYYGDVAGMRAAADYHAVKLPAAWVMEKAMTAPAEATALAATAPPPR
                     QTTGAPLVRYRDVHFAYPAVGGRTPRPIVRGVSAEIYPGDRIAVLGPNGSGKSTLVKQ
                     AIGLLRPQQGEVVVAGTPTTRQSVAQIARTLGYVFQNPGHMLFAPTVREELAFGPRNL
                     GRPAAQIPGDVDWALETTNLVPQADRPPLGLSFGQQKRVALASVLSMRPRVLIMDEPT
                     AGQDYANYTRFMDEIVALPDLEGVIFITHDLDLAIAYANRVWIIREGTLAADGTPEDV
                     LSRPALLQECGLLPTSLLEANLAALPQTGRFLRAELLAGAPGTAARFEDVGAYESGVQ
                     PTAR"
     gene            3034..3615
                     /locus_tag="VF276_01070"
     CDS             3034..3615
                     /locus_tag="VF276_01070"
                     /inference="COORDINATES: protein motif:HMM:NF018816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEX6605480.1"
                     /translation="MAQRVDITSGRENTNAWAVGTRQVVYMAIGAALYGLFSWATAGL
                     RIPGPFNSSIRPGVAIPLFFGAAFGPVVGFFTGFVGNIIGDLLSGYGFSFNWSLGNGI
                     MGLIAGLSALYVTKLDNPRAIGIAVGFALLGIVVGMGFASFTDIWFYGIALDAVWGEF
                     LPVAVTNAIAAIILIPILSIAWESVRGRTGQAV"
     gene            3682..4509
                     /locus_tag="VF276_01075"
     CDS             3682..4509
                     /locus_tag="VF276_01075"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HEX6605481.1"
                     /translation="MIANFSYVERDSPIHRLDPAAKVVFLLCYIFSMVLFLDVRVLLP
                     LMLLGFVYYRLAGLRWQETRRAWTFVLVFALVFVGLSAVLWGGGQQVAQPHVIWPGPF
                     GFDLTWEKLYYAAAVVMRMLGIAAVAIPLTFTTRPQDYGVAFKGLGLPDNFAVALDLA
                     LRLVPTFGDDFQSTLDAQRARGYETDALRGGPIAKLRRMAPLLVPVTINAIVGGEDII
                     NAMDLRAFGSGPRTWSRKRTRRPADRLVIAGGLLLLACALLWAFTGHGGFTVLWVPG"
     gene            4642..>5713
                     /locus_tag="VF276_01080"
     CDS             4642..>5713
                     /locus_tag="VF276_01080"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6605482.1"
                     /translation="MAPNPTAEWIVDEVVGEAFARPVALNTTIHPDDQMLSFAAGVRG
                     SEEIALADYFRSGGALLNAVRQLVTWKFGSFDQVAAFLDFAGGYGRLTRFLVHELPPD
                     RIWIAEIQPEAVAFQQAQFGVHGILSATNPADLACDQRFDCIFVASLFTHLPEATFTA
                     WLTRLYSLLNPGGLLIFSVHDAALLTPGEVMPESGLYFREASEIASLDTREYGMSIVT
                     EAFVRAAVQQATGRAVYHRIPYGLQYHQDLYLLANEPQPDFSTLHYRYGPYGFVDAGK
                     WVARDQLWMRGWAIDATPGAAVQEIQVLVDGRLGQKCLPFTERPDVDAQFPGAGASHA
                     GWACSCYVPDATPQSRLEVKVVS"
BASE COUNT          840 a         1972 c         1919 g          982 t
ORIGIN      
        1 tgggccatcg gcgtggtcgg cacccgccag gtcacgtcct atggccggga agtggccgcc
       61 cgcctggcgg gtgacctggc ccgcagcaag gtcacggtgg tgagcgggct ggcgcgcggc
      121 gtcgatacgc tggcccacac tgcggcgctc gatgcggacg ggcgcacggt ggccgtgctc
      181 ggctcgggcc tcgaccagct ctaccccgcc gagaaccgca ccctggcccg gcggatcgcc
      241 gacgggcgcg gcgcggtgat caccgattac ccgctcggca cccgccccga agcaatcaac
      301 ttcccgccgc gcaaccgcat catctccggc ctgagcctgg gtgtgctcat cgtggaggcc
      361 ggccccggca gcggcgccct gatcactcac cgcttcgccc tggagcaagg gcgcgaggtc
      421 ttcgccgtgc ccggctcgat cttcaacgtg caaagtcagg gcaccaacga tctgctcaag
      481 caaggggcca agtgcgtgac gcgggtggaa gacatcctcg aagagctgaa gatgggcctg
      541 gtggccgagc atgtgcaagc ggtcctcgcc ctgcccgaag accccaccga gcgcacgctg
      601 ctggcgtggt tgcaacgcga cgccgagccg cagcacatcg acgccatcgt gcgcggctgc
      661 aacctgcccg cctacgttgt cagcagcacg ctgaccatga tggaactcaa gggcctggtg
      721 cgccaggtcg gggcgttgca gtacgtgccg gtgcgctgag ggcgccgggc gatcccgcat
      781 ggcacgcgtt tgacaggtgc cgggggctgt gctacaatcg gcccaagtga agaaccacta
      841 tcacgagagt gcgagggatc tggcttgtgg accactcggc aacctgcagc gtggcaaggt
      901 gccccatcca gccctggtag ggaagatagg ccgcgccgcg gaacccgcaa cgcactgatg
      961 cctcttcccg ccgggagagg ttttttgttg acgtgagaat cgtgcttatg ccgcatacgt
     1021 ttcgcccggc aacgccgccc gtgcgcgctg ttgccgccca tgcccaatac tctgcatcgg
     1081 tgccgcacca cgggcaggcg ctggagccag gctgtctctg ttgtggccgg gagcggggcg
     1141 ctacgagccg gcgcgcggtc ccctgatcgg ggccggcatc cggcacaagc cagcgtctag
     1201 cccgtcgtcc tgtccccgga tggcgggttt tgtttcgcac cgccctgagc cgtcccagat
     1261 ggaaccatga tcgtgaatga aatcgtcgcc caaccaccgg ttatcgaagt cgcggaactg
     1321 agctttcagt atgcggcagc cggcacgcgc acgctcgaca atatctcgtt ccaggtgccg
     1381 gcgggcagcc tgttgctggt ggccgggcca agcggctgcg gcaaatcgac cctgctgcgc
     1441 tgcctcaacg gcctgatccc gcatagctac aagggcacgc tctggggcga ggcgcggctg
     1501 ggcggggagg acatcttcca cctgagccgg gccgcgctgg cgcggcgcgt gggcaccgtg
     1561 ttgcaggacc cgtcgcggca actggtcgcc agcacggtgg gcagcgatgt ggcctttggc
     1621 ccggagaacc tgggtttgcc ggtcgatgaa atcgaggcgc gcgtcacgcg ggcgctgggg
     1681 ctgctcggcc tggagggctt gcgcgagcgc gaaacgcagg cgctctccgg tggtgagcac
     1741 cagaaggtcg ccatcgcggg cgtgctggcc ttggacccca ccgtgctgct gcttgacgag
     1801 ccgttggcaa acctcgaccc gcgcagcgcc tgggaggccc tgcaactctt ccggcggctg
     1861 gccgactcgg gccgcagcat catcctggtg gagcaccggg tggaggatgc gctgaaaatc
     1921 gcgcccgagc aggtgatcct gatggacagc gggcgcatca cctactacgg cgacgtggcc
     1981 gggatgcgcg cggcagccga ctaccacgcg gtcaagctgc ccgccgcctg ggtgatggag
     2041 aaagccatga ccgcgcccgc tgaagcgacg gcgctagcgg cgaccgcgcc gccaccccgg
     2101 cagaccaccg gcgccccgct ggtgcgttac cgcgatgtgc acttcgccta ccctgccgtc
     2161 ggcgggcgca ccccgcggcc catcgtgcgc ggtgtgagcg ccgagatcta tccgggcgac
     2221 cgcatcgccg tgctcggccc caacggcagc ggcaagagca cgctggtcaa gcaggctatc
     2281 ggcctgctca ggccgcagca aggcgaggtc gtggtggcgg gcacgcccac gacgcggcaa
     2341 tcggtggccc agatcgcccg cacgctgggc tacgtcttcc agaaccccgg ccacatgctc
     2401 ttcgcgccga cggtgcgcga ggaactggcc ttcgggccgc gcaacctggg ccgcccggcg
     2461 gcgcagatcc ccggggacgt ggactgggcg ctggagacga ccaacctggt gccgcaggcg
     2521 gaccgcccgc cgctgggcct gtcgttcggc cagcaaaagc gcgtggccct ggcctcggtg
     2581 ctcagcatgc gcccgcgcgt cctgatcatg gacgagccga ccgccgggca ggactacgcc
     2641 aactacaccc ggtttatgga cgaaatcgtg gctttgcccg atttggaggg ggtgatcttc
     2701 ataacccacg acctggacct ggcaatcgcc tacgcgaatc gcgtctggat catccgcgag
     2761 ggcacgctgg ccgccgacgg cacgcccgaa gacgtgttga gccggcccgc gttgctgcag
     2821 gagtgcggcc tgctgccgac ctcgctgctg gaggcgaatc tcgcggcgct gccgcagacg
     2881 gggcgcttct tgcgcgccga attgctggcc ggggcgcccg gtaccgcggc ccggttcgag
     2941 gacgtgggcg catacgaatc gggtgtgcaa cccacagccc gctaatcaca gggactcgcg
     3001 cttagcaatt actacccgga ggaggaaaat ctcatggcac agcgtgtcga tattacatcg
     3061 ggccgcgaaa acacgaacgc ctgggcggtt gggacgcgcc aggtcgtcta tatggctatc
     3121 ggcgccgcgc tctacgggct gttctcctgg gccacggcgg ggctgcggat ccccggcccg
     3181 ttcaacagct cgatccgccc cggcgtcgcc atcccgctgt tcttcggcgc cgctttcggg
     3241 ccggtggtcg gcttcttcac cggcttcgtc ggcaacatca tcggcgacct gctgtcgggc
     3301 tatgggttct cgttcaactg gagcctgggc aacgggatca tgggcctgat cgccgggctg
     3361 tccgccctgt acgtgaccaa gctcgataac ccgcgtgcca tcggcatcgc cgtcggcttc
     3421 gccctgctcg gcatcgtcgt gggcatgggc ttcgcgtcct tcaccgacat ctggttctac
     3481 ggcatcgccc tcgacgccgt ctggggcgag ttcttgcccg tggccgtcac caacgccatc
     3541 gcggcgatta tcctgatccc gatcctgagc atcgcctggg aaagcgtgcg cgggcgcacc
     3601 ggccaggccg tctagcttag cggcgggcgg gacggcgctg gccaccgccg caggtcccgc
     3661 ccgcgttctc ctgggaacgt catgatcgcc aatttctcct atgtcgaacg cgacagcccg
     3721 atccaccggc tcgatccggc ggccaaggtg gtgttcctgc tctgctatat cttcagcatg
     3781 gtgctgttcc tggatgtgcg cgtgctgcta ccgttgatgt tgctcggctt cgtctattac
     3841 cggctcgcgg gcctgcgctg gcaggagacg cggcgcgcct ggacgttcgt gctggtgttc
     3901 gcgctggtgt ttgtcggcct cagcgcggtg ctctggggcg gcgggcagca ggtggcccag
     3961 ccgcacgtga tctggcccgg ccctttcggt ttcgacctga cctgggagaa gctgtactac
     4021 gcggcagccg tggtcatgcg catgctcggc atcgcggcgg tggcgatccc cctcaccttt
     4081 actacccgcc cgcaagacta cggcgtggcc ttcaagggcc tgggcctgcc cgacaacttc
     4141 gccgtcgcgc tggacctggc gctgcgcctg gtgcccacct tcggcgacga tttccagtcc
     4201 acgcttgacg cccagcgggc ccgcgggtac gagaccgatg ccttgcgcgg cggccccatc
     4261 gctaaactgc gccgcatggc cccgctgctg gtgcccgtga ccatcaacgc catcgtcggc
     4321 ggcgaggaca tcatcaacgc catggacctg cgcgcgttcg gctcgggccc gcgcacctgg
     4381 agccgcaagc gcacccgccg ccccgccgac cgcctggtca tcgccggggg cctgctgctg
     4441 ctggcctgcg cgctgctctg ggccttcacc ggccacggcg gcttcaccgt cctctgggtc
     4501 cccggctgac cctggcccga ctccgtaaat cttgacaagc cccgccggat gtcctagact
     4561 gaccccgcgt gatcgttgcg ccggtgcgcc gttcgcgtgc catccccatt tgtgctgatc
     4621 acgaggtagg atttgataga tatggctccc aacccaaccg ccgagtggat cgtggacgag
     4681 gtcgtgggcg aggccttcgc ccggcccgtg gccctgaaca ccacgatcca tcccgacgac
     4741 cagatgttga gctttgccgc cggggtgcgc ggcagcgagg aaatcgccct ggccgactac
     4801 ttccgctccg gcggcgccct gctcaacgcc gtccgccaac tggtgacctg gaagttcggg
     4861 tctttcgacc aggtggcggc tttcctcgat tttgccggcg ggtatggccg cctgacgcgc
     4921 ttcctggtcc acgagttgcc gccggatcgc atctggatcg ccgagatcca acccgaagcc
     4981 gtggcgttcc agcaggcgca gttcggcgtg cacggcatcc tctcggccac caatcccgcc
     5041 gatctcgcct gcgaccagcg gtttgactgt atcttcgtcg cgtcgttgtt cacgcacctg
     5101 ccggaagcga ccttcaccgc ctggctgacc aggctctaca gcctgctcaa tcccggcggg
     5161 ctgctgattt tcagcgtcca tgacgccgcg cttctgacgc cgggcgaagt catgccggag
     5221 tccgggctgt acttccgcga agcgagcgag atcgcgtcgc tcgatacccg cgagtatggg
     5281 atgagcatcg tcaccgaggc gtttgtgcgg gccgcggtgc aacaggcgac ggggcgcgca
     5341 gtctaccatc gcatcccgta tggcttgcag taccaccagg atctctatct gctggcgaat
     5401 gagccgcagc ccgatttttc cacgctgcac taccggtacg gcccctatgg cttcgtcgat
     5461 gccggcaagt gggtggcgcg cgaccagctc tggatgcgcg ggtgggccat cgacgcgacg
     5521 ccgggggcgg cggtccagga aatccaggtc ctcgtggatg ggcggctggg ccagaagtgc
     5581 ctgcctttta ccgagcgccc ggatgtcgat gcccagttcc ctggtgccgg cgcgtcgcac
     5641 gccgggtggg cctgttcgtg ctatgtgccc gacgccacgc cccagagccg gttagaggtg
     5701 aaagtcgtct cga
//
LOCUS       DASUYC010000068         2142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1004898, whole genome shotgun sequence.
ACCESSION   DASUYC010000068 DASUYC010000000
VERSION     DASUYC010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004898"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VF276_01085"
     CDS             <1..435
                     /locus_tag="VF276_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HEX6605483.1"
                     /translation="ALYNRAAGDPATAEPIARAVRRFSADLILVGLAGSLMLDAGRAA
                     GLRVAAEAFADRAYEADGTLRSRRLPGAIHPDPATAAAQALSIVRDGQVRAYDGTLVP
                     IRADTLCIHGDTPDAPAYARAVRAALEGAGIAVAPLAPVART"
     gene            443..1111
                     /gene="pxpB"
                     /locus_tag="VF276_01090"
     CDS             443..1111
                     /gene="pxpB"
                     /locus_tag="VF276_01090"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEX6605484.1"
                     /translation="MTPSPAIRPFGDSALLVVFGDSIDVALNSRVHALAAAVGALRAA
                     PDAPWGVPVPAYSSLLVPYDPQRLSYAEAEAALAPLVAEAEAALADETPGEVVEIAVR
                     YGGDDGPDLANVAARHDLTPAEVVALHTGALYRVFMLGFAPGFAYLGPLPPQLVTPRR
                     PTPRPRVPPGSVAIAGSQTGIYPLATPGGWHLLGRTEAALWDPHRDPPALLRPGQFVR
                     FVAR"
     gene            1111..2130
                     /locus_tag="VF276_01095"
     CDS             1111..2130
                     /locus_tag="VF276_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEX6605485.1"
                     /translation="MLEVVSPGLLSTVQDAGRTGYADLGVPGAGACDPHALAVANLLL
                     DNPPGAPALEITIAGPEFTVDSTCVIALAGADLGAHLVEAERPLAPGGAYLLRGGVTL
                     AFAGRDVGARAYLSLPGGIAVPPVLGSASTYLAGGFGGLDGRPLRAGDTLRPVQPGDF
                     HAAGRRWPAPSLLSPFPLREGGRGVRPVVTVRLVPGPHAHHFAPDALETLLAATWEVG
                     PQSDRMGLRLRGPVLLHSAPARAELVSQGIVWGALQVPADGQPIALLADHQTVGGYPV
                     LAVAIRADWPLLAQLPPGAPVRFALTTIAEAQVAYRAQQVALRSAAARLQRPELWARL
                     LAGTE"
BASE COUNT          222 a          829 c          801 g          290 t
ORIGIN      
        1 gcactctaca accgcgccgc gggcgacccg gcgacggcgg agccgattgc ccgcgccgtg
       61 cgccgcttct cggcggacct gatcctggtc gggctggccg ggtcgctgat gctcgacgcc
      121 gggcgggcgg cgggcctgcg cgtggccgcc gaagccttcg ccgaccgcgc ctacgaggcg
      181 gacggcacgc tgcgctcgcg gcgcctgccc ggcgccatcc accccgaccc ggccaccgcc
      241 gccgcccagg cgctgtccat cgtgcgcgac ggccaggtgc gcgcctacga cggcacgctc
      301 gtgcccatcc gggccgacac gctctgcatc cacggcgaca cgcccgatgc cccggcctat
      361 gcccgcgccg tgcgcgccgc gctggaaggc gcaggtatcg ccgtggcgcc gctggcgccg
      421 gtggcgcgga cctgagcggc gcgtgacccc ttccccggcc atccgcccct ttggcgacag
      481 cgcgctgctg gtggtcttcg gcgacagcat cgacgtggcg ctgaacagcc gggtccacgc
      541 gctggccgcc gccgtgggcg cactgcgcgc cgcaccggat gcgccgtggg gcgtgccggt
      601 gcccgcgtac agcagcctgc tcgtgcccta tgacccgcaa cggctgtcct acgcggaagc
      661 ggaagcggcg ctggcgcccc tggtcgccga ggcggaggca gcgctggcgg atgagacgcc
      721 gggcgaggtg gtagagatcg cggtgcgtta cgggggcgac gacgggccgg acctggccaa
      781 cgtggcggcg cggcacgacc tgacgcccgc cgaggtcgtg gccctgcaca ccggcgcgct
      841 ctatcgggtg ttcatgctcg gctttgcgcc cggcttcgcg tatctcgggc cgctgccgcc
      901 gcaactggtg acgccgcggc gccccacccc gcgcccgcgg gtgccgccgg gcagcgtcgc
      961 catcgccggg agccagaccg gcatctaccc cctggcgacg ccgggcggct ggcacctgct
     1021 gggccgcact gaggccgcgc tctgggaccc gcaccgcgac ccgcccgcgc tgctgcggcc
     1081 cggccagttc gtccgcttcg tggcccgctg atgcttgaag tcgtcagccc agggctgctg
     1141 tccaccgtgc aagacgccgg gcgcaccggt tacgccgacc tgggcgtgcc gggcgcgggc
     1201 gcctgcgacc ctcacgcgct ggccgtcgcc aacctgctgc tcgacaatcc gcccggcgcg
     1261 cccgcgctcg aaatcaccat cgccgggccg gagttcacgg tggacagcac ctgtgtcatt
     1321 gccctggccg gggccgacct gggagcgcac ctggtggagg cggagcgccc gctggcgccc
     1381 ggcggcgcgt acctgttgcg tggcggggtg acgctggctt tcgccgggag ggacgtgggc
     1441 gcgcgggcct acctgagctt gccgggcggc atcgccgtcc cgccggtgct cggctcggcg
     1501 tccacgtacc tggccggggg cttcggcggg ctggacgggc gccccttacg cgcgggcgat
     1561 accttacgcc ccgtacagcc cggcgatttt cacgccgctg gccgccgctg gcccgcaccg
     1621 tctctgctct cccccttccc gctccgggaa gggggccggg gggttaggcc ggtcgtcacc
     1681 gtccgcctcg tgcctgggcc gcacgcacac cacttcgcgc cggacgcgct ggagacgctg
     1741 ctggcggcga cttgggaggt ggggccgcag agcgaccgta tgggcctgcg cctgcgcggg
     1801 ccggtcttgc tgcatagcgc ccccgcgcgc gccgaactgg tctcgcaggg catcgtctgg
     1861 ggggcgttgc aggtgcccgc cgacggccag cccatcgccc tgctggccga ccaccagacc
     1921 gtgggtggct acccggtgct ggcggtggcg atccgcgccg actggccgct gctggcccaa
     1981 ctgccgcccg gcgccccggt ccgcttcgcc ctcacgacca tcgcggaagc ccaggtggcc
     2041 taccgggcgc agcaggtggc gctgcggtcc gcagcggcgc ggttgcagcg cccggagctg
     2101 tgggctcgcc tgctggccgg gaccgagtag gccgggcggt ta
//
LOCUS       DASUYC010000069         5694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1927943, whole genome shotgun sequence.
ACCESSION   DASUYC010000069 DASUYC010000000
VERSION     DASUYC010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5694
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1927943"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..1079
                     /locus_tag="VF276_01100"
     CDS             231..1079
                     /locus_tag="VF276_01100"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012750704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX6605486.1"
                     /translation="MKAEQVTDPVAYHGEGPVWSERWGGLRWVDMLAGDVLALPADGV
                     VTRRHVGTVAAALRPRQQGGAVIGVERGFALEAADGALTYLPPLWSDAGVRMNEGACD
                     PDGRFYCGSMAYDQQPGAGALYRLDPGGVVHPVLRSVTVSNGLDWSPDGAQAYYNDTA
                     TYRVSIFDYDRDHGLTNRRLFVDLADEGLRPDGLTVDAQGGVWVALSNGGQVRCYTTD
                     GRLADVVEVPARKTTACAFGGPHLDVLFITTSRENLEPGTDPLAGALFRASPGITGRP
                     VREFAG"
     gene            1131..2573
                     /locus_tag="VF276_01105"
     CDS             1131..2573
                     /locus_tag="VF276_01105"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012948982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX6605487.1"
                     /translation="MIKVLLLFGATGDLAARFLLPALAELYDAGKLPAGFRVVGTARE
                     DWDDQAFRHYAAQSLDQYAKAQVSAAARTALVRSLHYREVDFNDPGTVAAVVAAAGGK
                     PAAGAAAEPVAAYLALPPAVFPSAVRALGAIGLPEGSRIVLEKPFGEDLQSAIALNAL
                     LARVTGVAGEQAVFRVDHALALSTVQNLLGVRLANRVLEPVWNSTHIAQVDILWDETL
                     AMEGRVSYYDKTGALKDVIQNHLLQILCLMAMEPPLTLGEEDLRNGKLAVLRAVRPMT
                     ASDVATRTRRARYTAGRLANTGGADGRTVPSYMDEEGVDPKRCTETFAEVVLELDSWR
                     WSGTRFLLRTGKALSERRKEAIVRFRPVPHLPFGAEAGPPPPNELRIGLDGPHDFSLR
                     LTGWAAGPPAHLAPLNLAADLPAPDLPAYSRVLMDVLRGDSTLSIRGDEAEQAWRVLT
                     PVLQGWAEDLVPLHTYPAGSSVAARAPAAG"
     gene            complement(2615..5089)
                     /locus_tag="VF276_01110"
     CDS             complement(2615..5089)
                     /locus_tag="VF276_01110"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6605488.1"
                     /translation="MEASPLFGDWLQQRRKALDLTQQALADRAHCGKSTIVHIESGAR
                     RPSRALAETLAAALAIPPEDRAAFVQFARTGQLPAAPAAPGAPATPARPAPGGDLAIP
                     RTPLVGRAEAVATLAAAVRRPDTRLLTLTGPPGVGKTRLAAAVAATLADAFADGVTLV
                     PLAPIRDPDLVASAIVQALDLPPGEAAPGARLVEYLADKQILLVLDNFEQVVSAAPAL
                     AEWLAACPGLKALVTSREALSVRGEQQFAVPPLALPDTARRADPAALAQVAAVALFVE
                     RAQAVQPAFALTEENAPAVAALCVRLDGLPLAIELAAARSKLLPPAAILARLESRLGL
                     LTGGPRDLPARQQTLRAAIAWSYDLLDPAERAFFARLGIFVGGCTFQAIESVCDCAAL
                     AEGGALDGVASLLDKSLLRQATAGAAEPRYLMLETIREYAAEQLRAGDEAGEIARRHA
                     AYYTALAEAAEPELTGAEQSNWVARLEEEHANLRAALAWALDHDPAAALRLGGALWRF
                     WWMRGHLAEGRRAIEAALARGGGEAPGLRAKAENAAGGLAWFQSDVPAAVAHYEASLA
                     LARAAGDALGLSRALGNLASALSTEGGDYTRAQALLEEGLALDRALGDDSLMAFSLGK
                     LADLAYFQGDIPRAAALYEESLALQRARNDVHSMAITLNSLGTMHTLLGDAPRATAQF
                     EESLTLFRGLDSKHGIAYNLHSLGDLARKQGDLARARALYGEAVTLFREVDYRRPLAT
                     SLAALGGVLLAQDQPEPAARVLGAVDALLTALDTHLDAAEQTDYDRHVAAAQAHLAPA
                     IWTAAWTAGSTLPLDQVLAGIVRDPA"
     gene            5358..>5694
                     /locus_tag="VF276_01115"
     CDS             5358..>5694
                     /locus_tag="VF276_01115"
                     /inference="COORDINATES: protein motif:HMM:NF028473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-60 repeat domain-containing protein"
                     /protein_id="HEX6605489.1"
                     /translation="MHKQRRTARALVPALLLAGVLTLGQGGASYAAPQAPQAPLTKAP
                     TTGAGSLDPTFGTGGMVEVNAYEPGSDQARAIAILPDGKILTGGYTQNGSASAVIRYL
                     PNGAVDNTFG"
BASE COUNT          805 a         1955 c         2088 g          846 t
ORIGIN      
        1 cggtgctgct gcacaagtcg gcgcactgca tgatctactc tccgcacacg taagccactc
       61 accgcgtgtg gcgccgatgc acggcgatag gcgcgatgcc ggcgaggccg ggaggcctcg
      121 gccaggcgcg acgtgctata atctatccag gtgatctcgt gttgcgcggc gcgtttcggc
      181 tctgtgccca gccacgggtg tcgctcatct acagaaaggc aggtacaggc gtgaaggcgg
      241 aacaagtgac ggacccggtc gcctaccacg gcgaaggccc cgtttggtcg gagcgctggg
      301 gcggcttgcg gtgggtggac atgctggccg gggatgtcct ggccctgccg gcagacggcg
      361 ttgtcacccg ccgccatgtg ggcacggtcg ctgcggcctt gcgtccgcgg cagcagggcg
      421 gcgcggtcat cggggtggag cgcggcttcg cactcgaggc ggctgacggc gcgctcacct
      481 acttgccgcc gttgtggagc gatgccggcg tgcggatgaa cgaaggggcc tgcgatcccg
      541 acggccgctt ctactgcggc tcgatggctt acgaccagca gcccggcgcg ggtgccctgt
      601 accgcctgga tcccggcggc gttgttcacc ccgtgctgcg gagcgtcacc gtctcaaacg
      661 ggctggactg gagcccggac ggcgcgcagg cctactacaa cgataccgcc acctatcgcg
      721 tctccatctt cgattacgac cgggaccacg gattaaccaa ccgccggctg ttcgtggatc
      781 tcgccgacga gggcctgcgc cccgatggcc tcacggtcga cgcgcagggg ggcgtgtggg
      841 tcgccctctc caacggcggg caagtgcggt gctacactac ggacggccgg cttgctgacg
      901 tcgtggaggt gccggcgcgc aagacgaccg cctgcgcctt cggtggcccg cacctggacg
      961 tactgttcat tactacatcg cgagagaacc tggagccggg caccgatccc ctggccggcg
     1021 cgctgttccg cgcatcccct ggaatcacag gccggcccgt ccgcgagttc gccggctgag
     1081 ctagcggctg ggccgaacct gctctggatt caaccgcaag gagcctggtc atgattaagg
     1141 tattgttgct gttcggggcg acgggggacc ttgcggcgcg ctttctgctg ccggcgctcg
     1201 ccgagttgta cgatgccggc aagctgcccg cgggcttccg ggttgtgggc accgcccgtg
     1261 aggactggga cgaccaggct ttccggcact atgctgcgca gagtcttgac cagtacgcga
     1321 aggcccaggt ttcggcggct gcccgtaccg cactggtgcg ttctctgcac taccgcgagg
     1381 tggatttcaa cgatcccggc accgtggccg cagtcgtggc ggcggccggt ggaaagcccg
     1441 ccgcgggggc tgccgccgag ccggtggccg cgtacctggc cctaccaccg gccgtgttcc
     1501 cgtccgcggt tagggcgctg ggcgcgatcg ggttaccaga aggcagccgg atcgtgttgg
     1561 agaagccgtt cggcgaggac ctgcagagcg cgatcgccct caacgccctg ctggcgcgcg
     1621 tgaccggcgt cgccggcgag caagcggtgt tccgggtgga ccacgccctg gcactttcaa
     1681 cggtgcagaa tctgctcggg gtgcgactgg cgaaccgggt cctggagccg gtctggaaca
     1741 gcacccacat cgcgcaagtg gacatcctct gggacgagac gctggcgatg gaaggccggg
     1801 tctcctacta cgacaagacg ggcgcgctca aagatgtgat ccagaaccac ctgctgcaga
     1861 tcctctgcct gatggcgatg gaaccgcccc taacgcttgg cgaggaggac ttgcgcaacg
     1921 gcaagctcgc cgtgctgcgc gcggtacgcc ccatgactgc gtcggacgtc gcgacccgca
     1981 cccggcgggc acgttatacc gccgggcggc tcgctaacac aggcggggca gacggcagga
     2041 ctgtcccgtc ctacatggac gaggagggcg tggatcccaa gcgatgtacc gagacattcg
     2101 ctgaggtcgt gctggaactg gacagctggc gctggagcgg tacccgtttc ttgttgcgga
     2161 ccggcaaggc gttgagcgaa cggcgcaagg aggccatcgt gcgcttccgg cccgtgccgc
     2221 acctgccttt cggtgccgag gccgggccgc cgcctcctaa tgagctacgc atcggcctcg
     2281 acggcccgca cgacttcagt ctgcggctca ccgggtgggc cgccggccca cccgcgcacc
     2341 tggcgcccct caacctggcc gcggatctcc ccgcgcccga tctgcccgcg tacagccgcg
     2401 tgctgatgga tgtcctgcgg ggcgacagca cgctgtcgat tcgcggtgat gaggccgaac
     2461 aagcctggcg cgtgctaacc cctgtgttgc aggggtgggc ggaggatctg gtcccgctgc
     2521 atacgtaccc ggccgggtca tccgtggcgg cgcgcgcacc agccgccggc taaaggtctg
     2581 cgtccccggc gcctctacaa acggccgagt cggatcaggc cgggtcgcgc acgatgccgg
     2641 cgagcacctg gtcgaggggc agggtgctgc ccgcggtcca ggcggccgtc cagatggcgg
     2701 gcgcgaggtg cgcctgcgcg gcggcgacgt ggcggtcgta gtcggtctgc tcggcggcgt
     2761 ccaggtgcgt gtccagggcc gtgagcaggg catcgacggc gccgagcacg cgcgccgccg
     2821 gttcgggctg gtcctgggcc agcaggacgc cgccgagcgc ggccaggctg gtcgccagcg
     2881 ggcggcgata gtccacctcg cggaacagcg tgaccgcttc gccgtagagc gcgcgggcgc
     2941 gggccaggtc gccttgcttg cgcgccaggt cgcccaggct gtgcaggttg taggcgatgc
     3001 cgtgcttgct gtcgagtccg cggaacaggg tcaggctctc ctcgaattgt gccgtggcgc
     3061 ggggcgcgtc gcccagcaaa gtgtgcatgg tgcccaggct gttgagcgtg atcgccatgc
     3121 tgtgcacgtc gttgcgggcg cgttgcaggg ccaggctctc ctcgtagagc gcggcggcgc
     3181 gcgggatgtc gccctggaag taggccaggt cggccagctt gccgagcgag aaggccatca
     3241 ggctgtcgtc gcccagggcg cggtcgaggg ccagcccttc ttcgagcagc gcctgggcgc
     3301 gggtgtagtc gccgccctcg gtgctcagcg cgctggcgag gttgccgagc gcccgcgaga
     3361 ggcctagcgc gtcgcccgcc gcgcgggcca gggccagact cgcctcgtag tgcgcgacgg
     3421 cggcgggcac gtcgctttgg aaccaggcca gccccccggc ggcgttctcg gccttggccc
     3481 gcaggccggg cgcctcgccg ccgccccgcg ccagcgcggc ctcgatggcc cgccgcccct
     3541 cggccaggtg gccgcgcatc caccagaagc gccagagcgc gccgcccagg cgcaaggccg
     3601 ccgccgggtc gtggtccagc gcccaggcca gcgcggcgcg caggttggcg tgctcctcct
     3661 ccagccgggc gacccagttg ctctgctcgg ctcctgttag ttccggctcg gcggcctcgg
     3721 ccaatgccgt gtagtacgcc gcgtgccggc gcgcgatctc gccggcttca tccccggccc
     3781 gtaattgctc cgccgcgtac tcgcggatgg tttcgagcat caggtagcgc ggctcggcgg
     3841 cgcccgccgt ggcctggcgc agcaggctct tgtccagcag cgaggccacg ccgtccagcg
     3901 ccccgccttc cgccaaagcg gcacagtcgc acacgctctc gattgcctgg aaggtgcagc
     3961 cgcccacaaa gatgcccagc cgggcgaaga aggcccgctc cgccgggtcg agcaggtcgt
     4021 agctccaggc gatggcggcg cgcagcgttt gctggcgggc gggcaggtcg cgcgggccgc
     4081 cggtgagcaa ccccagccgc gactccagcc gggccaggat ggccgccggg ggcagcagtt
     4141 tgctgcgcgc cgccgccagt tcaatggcga gcggcagccc gtcgagccgg acgcagagcg
     4201 cggccacagc gggcgcgttc tcctccgtca gcgcgaaggc gggctgcacc gcctgggcgc
     4261 gctccacgaa cagggccacg gcggccacct gcgccaacgc cgccggatcg gcccggcggg
     4321 cggtatcggg tagggccagc ggcggcacgg cgaactgctg ctcgccgcgc acgctcaggg
     4381 cctcgcggct cgtcaccagc gccttcagcc ccggacaggc ggctaaccac tcagccaagg
     4441 ccggggcggc gctcaccacc tgctcgaagt tgtccagcac cagcaggatc tgcttgtccg
     4501 ccaggtattc gaccaggcgc gcgccggggg cggcctcgcc gggcggcagg tccagcgcct
     4561 gcacaatggc ggacgccacc agatcggggt cgcggatggg cgcgaggggc accagcgtga
     4621 cgccgtcggc gaaggcatcg gccagtgtag cggccacggc ggcggcgagg cgcgtcttgc
     4681 ccacgccggg cgggccggtg agtgtgagca ggcgcgtatc ggggcggcgc acggcggcgg
     4741 ccagggtcgc caccgcttcg gcgcggccca cgagcggggt gcgcgggatg gcgagatcgc
     4801 cgccgggcgc cgggcgggcc ggggttgcgg gcgcgccggg agcggcgggg gcggcgggca
     4861 attggccggt gcgggcaaat tgcacgaagg cggcgcgatc ctccggcggg atcgccagcg
     4921 ccgccgccag cgtctcggcc agcgcccgcg agggccggcg cgcgcccgac tcgatgtgga
     4981 caatcgtgct tttgccgcag tgggcgcggt ccgcgagggc ttgctgggtc agatccaggg
     5041 ctttgcggcg ctgctgcagc cagtctccga acagcgggga tgcttccatc ggcgggcgga
     5101 ttctcctgtc gcgtccgtgc cgcacgccgc gccgggccta tgcagatggc gggtcggtgt
     5161 tgggtggatg gcgggatgag tcaggttttt gagacacttt ctgtccggat cattatagcc
     5221 gatcccatgc tttaataggg ccaggacaca acactcaggc ccggcacaac aggtcgcggc
     5281 agggggacag ggtccgcatt catcgcttat tcacgtgcga ccggcacacg acaacatcac
     5341 gcgaaaggat caagtagatg cacaagcaac gacgaacggc gcgggcgctg gtgcccgcgc
     5401 ttttgctggc cggggtgctg accctgggcc agggcggggc gagctatgcc gctccgcaag
     5461 cgccgcaagc tcccctaacc aaggcaccca cgaccggcgc gggcagcctg gaccccacct
     5521 tcggcacggg cggcatggtg gaggtgaacg cctacgagcc gggttcggac caggcgcgcg
     5581 cgattgccat tctgcccgac ggcaaaatcc tcaccggcgg ctacacgcag aacggcagcg
     5641 cctccgccgt gatccgctac ctgcccaacg gcgccgtaga caacaccttt ggca
//
LOCUS       DASUYC010000070         4601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1282699, whole genome shotgun sequence.
ACCESSION   DASUYC010000070 DASUYC010000000
VERSION     DASUYC010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4601
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282699"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="VF276_01120"
     CDS             complement(<1..748)
                     /locus_tag="VF276_01120"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HEX6605490.1"
                     /translation="MAKIYIGNYEAADKGLLDALRRLNDSYHVFVEFQIPNPRGQRQV
                     DFLVIREDPAKPCIFMLEVKNERRRLRGTINGPWEYEESPGMWRLLPVSNPRDQNPIQ
                     QAYNTARTMQAWLSSMRALIQDPDNVWSDIPTVFPRLVLPVAHADSQLQTDSFTWRFD
                     GYERCLDSLTTFIPRDPVPLSAAAIERLARHLGVQRLPDAEPVEVPTDLPTWLRSTLM
                     RLSDEVAGLHQEVRELRMALGRPAAHIPAGN"
     gene            complement(1093..2343)
                     /locus_tag="VF276_01125"
     CDS             complement(1093..2343)
                     /locus_tag="VF276_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6605491.1"
                     /translation="MPAEGTAPAQSAWQPANRRGLIVILVDTFFMWGGFFMVVPLISV
                     YYVDGLGWAAATVGLVLGVRQLAQQGLSPLSGMLADRLGAKGPICAGLAIRAVGFAAL
                     AWATTFPILLASVLLAALGGALFDSPKSAAIAALTDETNRGRFYSLLGVVSGLGVALG
                     TQVGALLLAVDFALVALLSAACFVATFVITLVLLPPVQIAAERRGLTAGFRLAFHDRP
                     FMTFTLLLLGYWFLWVQFSISLPLLSKDVSGTTDSVSWIYGVNAGMNIFLQYPLLRLV
                     ERRLRPLPTLILGIVVMAAGFGGLALIHTVPGLLFCVVLVSTGMLLAAPTQQTVTAGL
                     TNPAALGSYFGVSSLAVALGGGLGNVSGGLLYDLGRQFAAPALAWAVFALVGFATAAG
                     LGLTLLRRRAPVAPHDIPAPGPAR"
     gene            complement(2352..2567)
                     /locus_tag="VF276_01130"
     CDS             complement(2352..2567)
                     /locus_tag="VF276_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015903415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX6605492.1"
                     /translation="MKDGQCPKCGSHDVHVDRENNGYSTTLRTGGLSYARLDAYVCVN
                     CGYVENYLSDKEKLRQIAEKWPQVGEE"
     gene            2801..3988
                     /locus_tag="VF276_01135"
     CDS             2801..3988
                     /locus_tag="VF276_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX6605493.1"
                     /translation="MHVGINAHLLAFTGDYRAAGLSKHIADLIPALLALDDDTRYTIY
                     TGSGARHRPPDFACSPRARLSESRWPTLRPEPRILWEQTVLPVAARRDRLDVLHCPVL
                     IQPVVSPVPTIITVHDLIFLRYPERYPRAKRLYLTALARASVRRARRIIAVSEATRRD
                     LQALLGVPPQRVTVIPNGVDHGRYYPRPPAEIAAFRAAHDLPERMILYIGTLEPRKNL
                     PQLLRAYAAARPDLGGARLVIGGAKGWFYDEIFRVAQELGLDSGPHAVRFAGYIPDAE
                     QPLWYNSATAFVYPSVYEGFGLPPLEALACGIPVIAADRSALPEVVGDAGLLVDPDSE
                     AALAAALRAVVTAPALAARLRAAGPAQAARFSWPASAEATLAVYRAAARRPTRNRRTE
                     SHR"
     gene            3985..4209
                     /locus_tag="VF276_01140"
     CDS             3985..4209
                     /locus_tag="VF276_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605494.1"
                     /translation="MNDLGSILSFALPLAAVLIMGFFIIRRPGKMRTMTCTRCDGSGQ
                     VNEHWPDPSQPGGWHDVHGVCPKCGGVGKV"
     gene            4437..>4601
                     /locus_tag="VF276_01145"
     CDS             4437..>4601
                     /locus_tag="VF276_01145"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX6605495.1"
                     /translation="MDVISHSLDQTRRIGMRLAALLAPGDLILLEGPFGAGKTTLTQG
                     IARGLDVGSDY"
BASE COUNT          778 a         1637 c         1495 g          691 t
ORIGIN      
        1 ggttccccgc cgggatatgc gccgccggcc gtcccagcgc catgcgcagc tcgcgcactt
       61 cctggtgcag cccggccact tcatcgctca ggcgcatcag cgtcgagcgc agccaggtcg
      121 gcaggtcggt cggcacctcc accggctcgg cgtccggcag gcgctgcacg cccaggtgcc
      181 gggccagccg ctcaatcgcc gccgcgctca gcggtaccgg gtcccggggg ataaaggtgg
      241 tcaggctgtc gaggcagcgc tcatagccgt cgaagcgcca ggtgaagctg tcggtctgca
      301 actggctgtc ggcgtgtgcc accggcagca ccaggcgcgg aaagaccgtg gggatgtcgg
      361 accagacgtt gtccgggtcc tggatcagcg cgcgcatgct gctgagccag gcttgcatgg
      421 tgcgggccgt gttgtacgct tgctggatgg ggttctggtc gcgcgggttc gacaccggca
      481 gcagccgcca catccccgga ctctcctcgt actcccaggg gccgttaatg gtgccccgca
      541 accggcggcg ctcgttcttc acttccagca tgaagataca gggcttggcc gggtcttcgc
      601 gaatgaccag gaaatcgacc tggcgttgcc cgcgcggatt ggggatctga aactcgacga
      661 aaacgtgata actgtcgttt aaccggcgta acgcatccaa caaaccctta tcggcggcct
      721 catagttgcc gatgtagatt ttagccatag aagaaggtcc tctctcgaaa aatgaaaatt
      781 aaacgcaaag gtagataaac ccggcgcccg gtcacagacg gcgttcgcgc tgggggcacc
      841 cccgatccgc ccagagttgg gcataatcgg ccaccccaca aacgcccggc acggcacctg
      901 catcatatac accacagctt ctgctaccct ggtccggtac aatgcatgtt cctgttaaag
      961 tagcgacgac tggataggtg cgggccatac ccgcacggtg tttcattata catcaggccc
     1021 gcggctcgcg ccaatcggac accggcgcgg gcaacctcag ctatggcgcc ccagcaggcg
     1081 cacgggcacc gctcagcggg ccggaccggg ggccgggatg tcatgcggcg cgaccggcgc
     1141 gcggcggcgc agcagggtca ggcccaggcc cgccgcggta gcgaacccga ccagggcgaa
     1201 gacggcccag gccagtgcgg gcgccgcgaa ttgccggccc agatcgtaga gcaggccgcc
     1261 gctgacgttg cccagcccgc cgccgagggc cacggccagc gaactgacgc cgaagtagga
     1321 tccaagcgcc gcagggttgg tcaggcccgc cgtcaccgtc tgctgggtag gcgccgccag
     1381 cagcatgccg gtcgagacga gcaccacgca gaacagcagg cccggcacgg tgtggatcag
     1441 cgccaggccg ccgaagcccg ccgccatcac cacgatgccc aggatcagcg tgggcagcgg
     1501 gcgcagccgg cgctcgacca gccgcagcag cgggtattgc aggaagatgt tcatgcccgc
     1561 attgacgccg taaatccagc ttaccgagtc cgtggtcccg ctgacatcct tggacagcag
     1621 cggcagcgag atcgagaact gcacccacag gaaccagtag cccagcaaga gcagggtgaa
     1681 ggtcatgaag ggccggtcgt ggaaggccag gcggaacccg gcggtgaggc cccggcgctc
     1741 ggcggcgatc tggacgggcg gcagcagcac cagcgtgatg acgaaggtgg cgacaaaaca
     1801 ggccgcagac agcagcgcca ccagcgcaaa gtccaccgcc agcagcagcg cgcccacctg
     1861 cgtgcccagg gccacgccca gcccgctgac cacgccgagc agggagtaga accggccccg
     1921 gttggtctcg tcggtcagcg cggcgatggc cgccgacttg ggcgagtcaa acagcgcgcc
     1981 gccgagcgcg gccagcagca ccgacgccag cagaatcggg aacgtcgtgg cccaggccag
     2041 cgcggcgaag cccacagcgc ggatcgccag cccggcgcag atcggcccct tggcgcccag
     2101 gcggtcggcc agcatcccgc tgagcggact caacccctgc tgcgcgagct ggcgcacccc
     2161 cagcaccagc cccaccgtgg ccgccgccca gcccagcccg tctacatagt agaccgagat
     2221 caggggcacg accatgaaaa agccgcccca catgaagaac gtgtcgacca ggatgacgat
     2281 caacccgcgc cggttcgccg gttgccatgc gctctgagcc ggcgccgtac cctcagccgg
     2341 catgcgttgc gctattcttc gccaacctgc ggccacttct cggcgatctg ccggagcttc
     2401 tctttatccg agagatagtt ctccacgtag ccgcagttga cgcagacata agcgtcgagc
     2461 cgggcgtagc tcaggccgcc ggtgcgtaat gtggtgctgt agccgttgtt ctcgcggtcc
     2521 acatgcacgt cgtgggagcc gcatttgggg cattgaccgt ctttcatagc ttctcctgtc
     2581 cagatcactg tcatgctgaa cgtagtgaag catctcgttt cattggggtg agcgacgaga
     2641 ctcttcgctg cgctcagagt gacaagcctc tcgctgtcat gctgaacgca gtgaagcatc
     2701 tcgtctcctt gcccacgcgc cgagactctt cgctgcgctc agagtgacaa gaacgaggtg
     2761 cgagactact gtatgggccg catagagtat aatagccgtt atgcatgtag gaatcaacgc
     2821 gcacctgctg gcgttcaccg gcgattaccg ggccgccggc ctctccaagc atatcgccga
     2881 cctcatcccg gccctgctcg cgctcgacga cgacacccgc tacaccatct ataccggctc
     2941 cggcgcgcgc caccggccgc ccgacttcgc ctgctcgccg cgggcacgcc tgagcgagag
     3001 ccgctggccc acgctgcgcc ccgaaccgcg catcctctgg gagcagacgg tgctgcccgt
     3061 cgccgcccgg cgcgaccggc tcgacgtgct gcactgcccc gtgctcatcc agccggtggt
     3121 cagcccggtg cccaccatca tcaccgtgca tgacctgatc ttcctgcgct accccgagcg
     3181 ttacccgcgc gccaagcgcc tctacctgac ggcgctggcc cgcgcgtcgg tgcgccgcgc
     3241 ccgccggatt atcgccgtgt cggaggcgac ccgccgcgat ctccaggcgt tgctcggcgt
     3301 gccgccccag cgggtgacgg tcatccccaa cggcgtggac cacgggcgct actacccgcg
     3361 cccgcccgcc gagatcgccg ccttccgcgc cgcccacgac ctgccggagc ggatgatcct
     3421 ctacatcggc acgctggagc cgcgcaagaa cctgccgcaa ctgctgcgcg cctacgccgc
     3481 cgctcgcccg gacctgggcg gggcgcgcct ggtgatcggc ggggccaaag gctggttcta
     3541 cgacgagatc ttccgcgtgg cccaggagct ggggttggat agcggccccc acgccgtgcg
     3601 cttcgccggc tatataccgg acgccgagca gcccttgtgg tataatagcg ctacggcttt
     3661 cgtctatcca tccgtctacg agggcttcgg cctgccgccg ctggaagcgc tggcatgcgg
     3721 gattccggtc atcgccgcgg atcgctcggc gctgccggaa gtcgtgggcg acgcgggcct
     3781 gctggtggac ccggactcgg aggcggccct ggccgcggcg ctgcgcgcgg tggtcaccgc
     3841 cccggccctg gccgcgcggt tgcgcgccgc cggccccgcc caggcggccc gcttttcctg
     3901 gcccgcgagc gcagaggcca cactcgccgt ctatcgcgcc gccgcccggc gccccacccg
     3961 taatagaagg actgaatcgc accgatgaac gacctgggca gtatcctgag ttttgcgctt
     4021 ccgctggccg ccgtgctgat catgggcttt tttatcatcc gccggccggg caagatgcgc
     4081 accatgacct gcacccggtg cgacggcagc gggcaggtca acgagcattg gcccgacccc
     4141 tcccagccgg ggggctggca cgatgtccac ggtgtgtgcc ccaagtgtgg cggggtgggc
     4201 aaggtttaac ccgcgccgcc cgttgcaggg tggggcatgg gccgttgtaa aagagaacgg
     4261 cggatagggg caacccggta tacaggaggg tggatgcgct caccggcccg cagcccgcac
     4321 acacgccggg gccgggcgcg cccgtagtag ctatgcccgt ttttcgcaca cccagccccc
     4381 agagtcgccc gcccggcccc gccggtccgg ggcgccccgc gcggccgaca cgcgatatcg
     4441 acgtgatcag ccatagcctg gaccagaccc ggcgcatcgg catgcgcctg gccgccctcc
     4501 tggcgcccgg cgacctgatc ctgctcgaag gtccctttgg ggcgggcaaa accacgctca
     4561 cccagggcat cgcccgcggt ctcgacgtgg ggtccgacta c
//
LOCUS       DASUYC010000071         3567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1465306, whole genome shotgun sequence.
ACCESSION   DASUYC010000071 DASUYC010000000
VERSION     DASUYC010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1465306"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VF276_01150"
     CDS             <1..536
                     /locus_tag="VF276_01150"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX6605496.1"
                     /translation="GVFPLVPSELILPLAGSLASRGQLLLPLLLLAAVLGSLASATLL
                     YSIARWGGEPLIGRLLDRWGRWILMSREDLDHTRAWFNRRGHIMVLLGRFTPGLRTLI
                     SVPAGLTGMPYPKFALYTALGSSIWTGGLLVAGWLLGENWPRVQGWVAPIAPLIYLTL
                     LILVAVFFWRRWSARRG"
     gene            651..3251
                     /locus_tag="VF276_01155"
     CDS             651..3251
                     /locus_tag="VF276_01155"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HEX6605497.1"
                     /translation="MIPLRLTLKNFMSYRDGSPPLDFAPIHTACLSGENGAGKSALLT
                     AITWALWGATPVHADDEDLIAQGETEMSVDFEFGLGDQVYRVLRSRTRKGKTTTGKLY
                     FEMQGPDGGWRPISGNSARETQRLIIERLRMDYDTFVNSAFLRQGRADEFTTKNATER
                     KEILAKILGLEEYDVLAERAREQARSRESDRRLLAAHIQQLDIQIARKPEVQAQLAEL
                     AGYLSQARELLAVATTEAERLHLQVAALEKDKLELENVRARMAQDEVEISKADKAIAV
                     LEANLLRYREVLAQRDGITTGYQRLQMLRTRERELSDLARQSRDLEGEIHRLERAIDH
                     ERSGLLNQIGLVERTISESRRVLETRPDLERRLGEVRVELAHFEALDQEWRAAEVERD
                     TLQQRQAGLVGANDKLKEEADAIKRKLDMLNEAVAAGPTGHQHGRANCPLCNGLLDAD
                     ALARVRASYEQDITARRQQWKTNKRECETLDKQIATLASRIADLEQKLKARKMAEKRE
                     ATYERELQSTVEAETRLARETDNLQALQVRLDMADYAHAERQALAEANARLTALGYDA
                     QEHRRVATESQQLNDYERRFGDLRSAEEKQESDTERLAREKENREGHAARLTRERAEE
                     VRLAAAVYELAERRREAGEQDSKLRTERDRFERLSREQAALDRELEGIASAESECAIK
                     AAELTLASSEKTIYEDLAKAFGKGGIQAMIIEDVVPELAEEANRLLSRMTDGRMQLAF
                     ETQRQARSKDGVIETLDIKITDGAGVARKYEMFSGGEAFRINFAVRIALSRLLAHRAG
                     AQLQTLIIDEGFGTQDAQGRERLVQAIRTIQDDFEKILVITHIDELKDEFSARIDVVK
                     TPRGSQAVVN"
     gene            3256..>3567
                     /locus_tag="VF276_01160"
     CDS             3256..>3567
                     /locus_tag="VF276_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605498.1"
                     /translation="MAEKNQPAAPVGAAVPPKATAATPRLRRAAPRPAPAPETGAVAA
                     AQPKRTRRRAPRPPRPAPPPSYLWGVGLAGAGALLILAGLLATHDVETMANPWAPWRV
                     AV"
BASE COUNT          620 a         1242 c         1216 g          489 t
ORIGIN      
        1 aaggcgtctt cccgctggtg ccgtcggagc tgattctgcc gctggccggg agcctggcct
       61 cccgcgggca gctcttgttg cccttgctgt tgctggcggc ggtgctgggg tcgcttgcca
      121 gcgccacgct gctctacagc atcgcccgct ggggcggcga gccgctgatc gggcgcctgc
      181 tcgaccgctg gggccgctgg atcctgatgt cgcgcgagga tctggaccac acgcgcgcct
      241 ggttcaatcg tcgcggccat atcatggtgc tgcttggccg cttcacgccg gggctgcgca
      301 cgctgatctc ggtgcccgcc ggcctgaccg ggatgcccta tcccaagttc gcgctttaca
      361 cggcgctcgg cagtagcatc tggaccggcg gcctgctggt cgcgggctgg ctgctcggcg
      421 agaactggcc ccgcgtgcag ggctgggtcg cgcccatcgc cccgctcatc tacctcaccc
      481 tgctcatcct cgtcgctgtc ttcttctggc ggcgctggtc ggcccggcgc ggctgaaccc
      541 ccggttatgg tataattagg cgttatagta acgcctctcc ggcctgtttt gcgggctgtt
      601 gtgtgtgact gcccggttcg taccgacacc tgaaggagtc tattcgccgc atgattcccc
      661 tgcgtctcac gctcaagaac tttatgagct atcgcgacgg cagcccgccg ctcgacttcg
      721 cgcccatcca taccgcgtgc ctctccgggg agaacggggc cggcaaatcg gccctgctca
      781 cggccattac ctgggcgctg tggggcgcga ctccggtcca cgccgacgac gaagatctga
      841 tcgcgcaggg cgagaccgag atgtcggtcg atttcgagtt cggcctgggc gatcaggtct
      901 accgcgtgct ccgcagccgc acgcgcaagg gcaagacgac caccggcaag ctctacttcg
      961 agatgcaggg ccccgacggc ggctggcgcc cgatcagcgg caactcggcc cgcgagaccc
     1021 agcggctgat catcgagcgg ctgcgcatgg actacgacac cttcgtcaat tcggccttcc
     1081 tgcgccaggg gcgcgccgac gagttcacca ccaagaacgc caccgagcgc aaggagatcc
     1141 tcgctaagat cctgggcctg gaggaatacg acgtgctggc cgagcgcgcc cgcgaacagg
     1201 cccgctcgcg cgagagcgac cgccgcctgc tggccgccca catccagcaa ctggacatcc
     1261 agatcgcgcg caagcccgaa gtgcaggccc aactggccga actggccggc tatctgagcc
     1321 aggcgcgtga gctcctggcc gtggcgacga ccgaggccga gcggttgcac ttgcaggtgg
     1381 ctgcgctgga gaaggataag ctcgaactgg aaaacgtccg tgcccgcatg gcgcaggacg
     1441 aggtcgagat cagcaaggcc gacaaagcca tcgccgtgct cgaagccaac ttgctgcgct
     1501 accgcgaggt gctggcccag cgcgacggca tcacgaccgg ctaccagcgc ttgcagatgc
     1561 tgcggacccg cgagcgcgaa ctgagcgacc tggcccgcca gagccgcgac ctggaaggcg
     1621 agatccaccg cctggagcgc gcgatcgacc acgagcgcag cggactgttg aaccagatcg
     1681 gcctggtcga gcgtaccatc agtgagagcc gccgcgtgct ggagacgcgc ccggacctgg
     1741 agcgccggct cggcgaggtg cgcgtcgaac tggcccactt cgaggcgctg gaccaggaat
     1801 ggcgcgccgc cgaggttgag cgcgacacgt tgcagcagcg ccaggccggg ctggtgggtg
     1861 ccaacgacaa gctgaaggaa gaagccgacg ccatcaagcg caagctggac atgctcaacg
     1921 aagccgtggc cgccggcccg accgggcacc agcatgggcg ggccaactgc cccctctgca
     1981 acggcctgct cgacgccgac gcgctggccc gtgtgcgcgc cagctacgag caggatatta
     2041 ccgcgcgccg ccagcagtgg aagacgaaca agcgcgagtg cgaaacgctg gacaagcaga
     2101 tcgccacgct tgcctcgcgc atcgccgatc tggagcagaa gctgaaggcg cgcaagatgg
     2161 ccgagaagcg cgaggccacc tatgagcgcg aactccagag caccgtggag gccgagaccc
     2221 gcctggcgcg ggaaaccgac aatctccagg cgctgcaggt ccgcctcgac atggccgact
     2281 acgcccacgc cgagcgccag gccctggctg aggccaacgc ccgcctgacc gcgctgggct
     2341 acgacgcgca ggagcaccgc cgggtggcga ccgagtcgca gcaactgaac gactacgagc
     2401 ggcgctttgg cgacctgcgc tcggcggagg agaagcagga gtcggatacc gagcgcctgg
     2461 cccgcgagaa agagaaccgc gagggccatg ccgcccgcct gacccgcgaa cgggccgaag
     2521 aggtgcgcct ggccgccgcg gtctacgaac tggccgagcg gcgccgcgag gccggcgagc
     2581 aggacagcaa gctacgcacc gagcgcgacc ggtttgagcg cctttcacgc gagcaggcgg
     2641 ccctggaccg cgaactggag ggcatcgcca gcgccgaaag cgaatgcgcg atcaaggccg
     2701 ccgagttaac cctggctagc tcggaaaaga cgatctatga ggacctggcg aaagccttcg
     2761 gcaagggcgg gattcaggcc atgatcatcg aggacgtggt gcccgaactg gccgaggagg
     2821 ccaaccgcct gctgagccgc atgaccgacg ggaggatgca actcgccttc gagacgcagc
     2881 ggcaggcgcg cagcaaggac ggcgtcatcg aaacgcttga cataaagatc accgacgggg
     2941 ccggggtggc gcgcaagtac gagatgttca gcggcggcga ggctttccgc atcaacttcg
     3001 ccgtccgcat cgcgctcagc cgcttgctgg cccaccgcgc gggcgcccag ttgcagacgc
     3061 tgatcatcga cgagggcttc ggcacccagg acgcgcaggg gcgcgagcgc ctggtgcagg
     3121 ccatccgcac catccaggac gacttcgaga agatcctggt cattacccat atcgacgagt
     3181 tgaaggacga attttccgcg cgcatcgacg tagtcaagac gccgcgcggc tcgcaggccg
     3241 tggtgaactg agcgtatggc cgagaaaaac cagcccgccg cccccgtcgg cgcggcggtg
     3301 cccccgaaag cgaccgccgc caccccgcgg ttgcgccggg ccgccccgcg ccccgcgccc
     3361 gcgccggaga ccggcgccgt agcggcagcg cagccgaagc gcacccgccg ccgggcgccc
     3421 cgtccgccgc gccccgcgcc gccgcccagc tacctctggg gcgtgggcct ggccggggcg
     3481 ggcgcgctgc tgatcctggc cggcctgctg gcgacccacg atgtcgagac catggcgaac
     3541 ccctgggccc catggcgggt cgccgtc
//
LOCUS       DASUYC010000072         2625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1748011, whole genome shotgun sequence.
ACCESSION   DASUYC010000072 DASUYC010000000
VERSION     DASUYC010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1748011"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2625
                     /locus_tag="VF276_01165"
     CDS             <1..>2625
                     /locus_tag="VF276_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605499.1"
                     /translation="VAYLGSVGGGVWKTTNCCGSATSWSAVTDDPLLTTISIDDITID
                     PSNHNTVYAGTGDLNYGSFSMGSQGILKSTDSGASWTVIGADVFGAALPQPPGNFPQY
                     QAVGKVRVDPRNSNNVVAGTKTGLYFSYDAGANWTGPCTTNSFSTQRQDITDLALNNI
                     GGSTRIIAAVGVRGFATPVQYNLDQNGANGIYKGTLPASGCPTDFTLISTNGNGWAGM
                     NAGSGTPYAGAGSGDQLGRINLAVAPSNPNYIYAQVQAITPSSANGCGGAQGCQLAVW
                     RTTNGGTTWTQLPGSLGSQLKNCANGGGDYSQNWYDQAVAVDPNNPDRVFISTFDIWF
                     WSAGATSFNDISCGYSYSGSAGPVHVDQHALAFVPGSSSILLAGHDGGVNMTGNAQQV
                     TSTVDPTWVNLDNGLNTIEFYSGDISGFFATSPVQMANGGAQDNGSSTVKYTNGAPGP
                     AQWQMGTGGDGFYARIDPVAGRFWQGGNSGGVTRCIAADCTQPGTSWATARGAWTGDT
                     QAFQLPYEIFKGTPGDPANDCAPAPAGCNHLIAGTIRVWETINGDAAAGTPVRNSWYV
                     NSPTNLTKQTLGNRSYINQLAYAPSISTTAIVGTNDGNVQYGFGLGRGPLNPATWVNV
                     TGGNTVLPNRPILDVTIDPTTPTTAYASVGGFNANTPSTPGHIFQVVCSANCAAFTWT
                     DKTGNLPDIPADSIIANPRYPQQVFAGTDFGLYFTNDITAASPVWYRFQNGLPNVMIW
                     DMQIDRGATTLSVWTRGRGAYVWPLPDSPVKGSQAISFAALADKTFGDADFTVNASAT
                     SGLPVSLSPAGACTVSGSTVHITGAGSCTITATQDGDENYYPATPVVRSFNIARLTTT
                     TQLSVTPSSVQYSDVVTI"
BASE COUNT          490 a          980 c          773 g          382 t
ORIGIN      
        1 tgtggcctac ctgggaagcg tgggcggcgg cgtttggaag accacgaact gctgcggcag
       61 cgccacctcg tggagcgccg tcaccgacga cccgctgctc accacgatca gcatcgacga
      121 catcactatc gacccgagca atcacaacac cgtatatgcc ggcaccggcg atctgaacta
      181 tggctccttc tccatgggca gccagggcat cctcaaatcg accgactccg gcgcgtcctg
      241 gacggtgatc ggcgccgatg tgttcggcgc ggccctcccg cagccgcccg gtaacttccc
      301 gcagtatcaa gccgtcggca aggtccgggt ggacccgcgc aacagcaaca acgtcgtggc
      361 gggcaccaaa accggcctct atttctcata tgacgccggc gccaactgga ccggcccctg
      421 cacgacgaac agcttcagca cgcagcgcca ggacatcacc gacctggccc tgaacaacat
      481 cggcggcagc acgcgtatca tcgccgcggt cggcgtgcgc ggctttgcca cccccgtgca
      541 gtacaacctc gatcagaacg gcgcgaacgg catctacaag ggcacgctcc cggccagcgg
      601 ctgcccgacc gactttaccc tgatcagcac caacggcaac ggctgggcgg gcatgaatgc
      661 cggcagcggc accccctacg ccggggcggg cagcggcgac cagcttggcc gcattaacct
      721 cgccgtggcg ccgagcaacc cgaactatat ctatgcgcag gtgcaagcca tcacgcccag
      781 cagcgccaac ggctgcggcg gcgcgcaggg ctgccagctc gcggtttggc gcacgactaa
      841 cggcggcacc acctggacgc agctccccgg ttcgctcggc tcgcaactca agaactgcgc
      901 gaacggcggc ggcgactaca gccagaactg gtacgaccag gccgtcgcgg tcgatccgaa
      961 taaccccgat cgcgtcttca tttccacctt cgacatctgg ttctggagcg cgggcgcgac
     1021 ctcgttcaac gacattagct gcggttacag ctatagcggc agcgccggcc ccgtgcatgt
     1081 ggaccagcac gccctggcct ttgtgcccgg ctcctccagc atcttgctgg ccggccacga
     1141 cggcggcgtg aacatgaccg gcaacgccca gcaggtcacc tcgaccgtcg acccgacctg
     1201 ggtcaatctc gacaacggcc tgaacaccat cgagttctac tccggcgata tcagcggctt
     1261 cttcgccacc tcgccggtgc agatggcgaa cggcggcgcc caggataacg gttcctcgac
     1321 cgtcaaatac accaacggtg cgcccggccc cgcccagtgg cagatgggca ccggcggcga
     1381 cggcttctat gcgcgcatcg acccggtggc gggacggttc tggcagggcg gcaacagcgg
     1441 cggcgttact cgctgcatcg ccgccgactg cacccagccg ggcacctcct gggcgacggc
     1501 gcgcggcgcc tggaccggcg atacccaggc attccaacta ccctatgaaa tcttcaaggg
     1561 cacgcccggc gatccggcca acgactgcgc gcccgcgccc gccggctgca accacttgat
     1621 cgccggcacc atccgcgtct gggagacgat caacggcgac gcggcggccg gcaccccggt
     1681 ccgcaactcc tggtacgtca acagcccgac caatctgacc aagcaaacgc tgggcaaccg
     1741 ctcgtacatc aaccagcttg cctatgcgcc gagcatctcg accacggcca tcgtgggcac
     1801 caacgacggc aacgtgcaat acggcttcgg cctgggccgc ggccccctga acccggccac
     1861 ctgggtgaac gtcaccggcg gcaacacggt gctgccgaac cgcccgatcc tggacgtaac
     1921 catcgacccc accaccccga ccacggccta tgcctcggtg ggcggcttca acgccaacac
     1981 gccgagcacg cccggccata tcttccaggt cgtctgtagc gcgaactgcg cagcgttcac
     2041 ctggactgac aagaccggca acctgcccga catcccggcg gactcgatca tcgccaaccc
     2101 gcgctacccg cagcaggtgt tcgccgggac cgacttcggc ctctacttca ccaacgacat
     2161 caccgcggcc tcgccggtct ggtatcgctt ccagaacggg ctgccgaacg tgatgatctg
     2221 ggacatgcag atcgaccgtg gggccacgac tctctccgtc tggacgcggg gccgcggggc
     2281 ctatgtctgg ccgctgccgg actcgccggt caagggcagc caggccatca gcttcgccgc
     2341 cctggccgat aagaccttcg gcgatgcgga cttcaccgtg aacgcctcag caacctcggg
     2401 gctgccggtt agcctcagcc ccgcgggcgc ctgcaccgtc tccggcagca cggtccacat
     2461 caccggcgcc gggtcctgca cgatcactgc tacccaggac ggcgacgaga actactaccc
     2521 ggcaacgccc gtcgtccgga gctttaacat cgcccgcctg accaccacga cgcagttgag
     2581 cgtaacgccg tcgagcgtgc agtatagcga cgtagtcacc atcag
//
LOCUS       DASUYC010000073         2105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_540296,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000073 DASUYC010000000
VERSION     DASUYC010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540296"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VF276_01170"
     CDS             <1..492
                     /locus_tag="VF276_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605500.1"
                     /translation="LLDWNIYLTNVGAAQLTGEQVRQVYGARWQIELLFKLWKSAGEL
                     DNVRGQHTGRVLCELYAKLIGLAVFGYLSAPVRLAAAGDELSPLKAWQVWQRQIGPVA
                     QALRRGHGLAGILAQVYRLWQQFGRKERRRDRPASLQQLQRQPRRNPPPGYCRPLARP
                     AAA"
     gene            complement(812..1984)
                     /locus_tag="VF276_01175"
     CDS             complement(812..1984)
                     /locus_tag="VF276_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605501.1"
                     /translation="MFRHTTGWNRRLYVLIRACAALCLAVLGLAVASGAPAAAQGVPD
                     RAGLPAGARPLSTARAHGSSVRRDTETIKYWSGSFTYQDTTYPFTMVGTDPAAGSKTT
                     SVPMKLIPVRLVFADGTVLDGSSKAGLTIQSPMFQATDVGAGTGGAAPHDVTQVADAI
                     QRAEFANYVSTTSPGYHVLLGGPALLATQTLHVPTDKGFVVQDSAGENIGLVDIDWFD
                     AQLVQLMSALHIKAVTVPIFLTNNTVLYIQNPNNCCVFGYHNALGSTSPSGKQQVQTY
                     IFSSYLTEHVFGTANLADIDGLSHEVSEWMNDPFVNNVVPPWSVPSQPQYGCTAVLET
                     GDPLVSVAFALTMPNGVTYHPQDEAFLPWFARQVPSTSYHGHYTYLATFADVSPPC"
BASE COUNT          319 a          659 c          799 g          328 t
ORIGIN      
        1 ctcttggact ggaacatcta cctgaccaat gtcggggcgg cgcagttgac gggggagcag
       61 gtgcggcagg tctatggcgc gcgctggcaa atcgaactgc tgttcaaact ctggaaaagt
      121 gccggggaac tggacaatgt ccgggggcag cacacggggc gggtgctgtg cgaactgtat
      181 gccaagttga tcgggctggc cgtgtttggc tacttgagtg cgccggtgcg cttggccgcg
      241 gcaggggacg agttgagtcc gctgaaggcc tggcaggtgt ggcaacggca aatcggcccc
      301 gtggcccagg cgctgcgccg ggggcacggg ctggccggca tcctggcgca ggtatatcgg
      361 ctctggcagc aattcggccg gaaagagcgg cgccgggatc ggccggcgag cttgcagcaa
      421 ttacagcgcc aaccccggcg gaatccgccg cccggttact gccgcccact cgcccggccc
      481 gcagcggcgt aaccgccgtg tgtgaaatcc ttaacttgac gcggatgggg tataccccac
      541 cccgccgcgg ggaggggcga tcgcttaccg ggatgccgcc tccggggcgg cgccgcccgg
      601 cccataaatg ggcgggctac gtcaacgaag gccgcctgcg cggcctgtct cggggcggcg
      661 agcccgcccg tggtgctccc cggcccgccg cgggcagggg ggttggcttg cccgccgcgg
      721 ggaggggcga tcgcttaccc cgccggtcgt cgccccccgg cccgccgcgg gccggggggt
      781 tggggggtca ggttggtccg cgcggccctc gctagcaggg cggcgacaca tcggcgaagg
      841 tcgccaggta ggtgtagtgc ccgtgatagc tggtggaggg cacctgccgc gcgaaccagg
      901 ggaggaacgc ctcgtcctgc gggtggtagg tcaccccgtt gggcatcgtg agcgcgaagg
      961 ccacgctcac cagcgggtcg ccggtttcca gcaccgcggt gcagccgtac tgcggctgcg
     1021 acggcaccga ccacggcggc accacgttgt tcacaaaggg gtcgttcatc cactccgaga
     1081 cttcgtggct caggccgtcg atgtcggcca ggttcgccgt cccgaacacg tgctccgtca
     1141 ggtacgacga gaagatgtag gtctgcacct gctgcttgcc gctgggcgac gtgctgccga
     1201 gggcgttatg gtagccgaac acacagcagt tgttggggtt ctggatatac agcacggtgt
     1261 tgttggtgag gaagatcggc acggtcaccg ccttgatgtg cagggcgctc atgagttgga
     1321 ccaattgcgc gtcaaaccag tcgatatcca ccagcccgat attctccccg gcggagtcct
     1381 gcacgacgaa gcccttgtcg gtgggcacgt ggagcgtctg ggtggcgagc agggccggcc
     1441 cgccgagcag cacatggtag ccgggactgg tggtcgagac gtagttggcg aactcggcgc
     1501 gctggatggc gtccgccacc tgggttacgt catggggcgc cgcgcccccg gtgcccgcgc
     1561 ccacgtcggt cgcctggaac atgggcgact ggatggtcag gccggctttg ctgctgccgt
     1621 cgagcaccgt gccgtcggcg aacaccaggc ggaccgggat gagcttcatc gggaccgagg
     1681 tcgttttcga gccggcggca gggtcggtgc cgaccatggt aaaggggtag gtggtgtcct
     1741 ggtaagtgaa cgacccgctc cagtacttga tggtctcggt gtcgcggcgg acggagctgc
     1801 cgtgggcgcg ggcggtggac agcggccggg cgccggcggg caggccggcc cggtcgggga
     1861 ccccctgggc ggcggcgggc gccccgctcg cgaccgccag gcccaacacg gcgaggcaga
     1921 gggccgcgca ggcgcggatc aggacataca gccggcgatt ccaacccgta gtgtggcgaa
     1981 acatgctggt attcctttcc atgcagtagc gggtgaagat catgcaggac cgcggaagcg
     2041 atccgcgggg gccggccact ggcgcgcgtg gaccacgccg ccggccgcgg cctcggcaac
     2101 gacgg
//
LOCUS       DASUYC010000074         2981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_803276,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000074 DASUYC010000000
VERSION     DASUYC010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803276"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VF276_01180"
     CDS             <1..732
                     /locus_tag="VF276_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605502.1"
                     /translation="LRIPSDPTRLSPPGPAAQLAAALNIVAGDILVWIDPKAGRMNAS
                     AIRALAGPLLHDPGVLLSKPFWPEPDSEGTGVPEGRVPNARFAPIRVTDLLAMSVPEL
                     AALPLYSWLRAFYPRLGAVLNPLGSTFAARVSLLRDLLPSLQATEDALGGDGARSATI
                     SPATAFAAGMLLETAALHSSRAIAQVEMAGSRRGPRGSQVANRDTRRLRQVAELLALF
                     ATRHDAIQRQAALQEIRGRILNATA"
     gene            882..2045
                     /locus_tag="VF276_01185"
     CDS             882..2045
                     /locus_tag="VF276_01185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6605503.1"
                     /translation="MPRGRKSSAQSSHPPPAVDVSGIGLDPEGEAAAKAEHDSPEGEA
                     LFQELKRMEEAAHTGADLATIVDGAPPTAAAAAPAPPVAVVAEEDEEEEESEPWVDEE
                     EALRHIDPAYVTDPVRLYLREISRAPLLSSQQEFALARAINEGDVEATQKFVLANLRL
                     VVSIAKKYVGRGLTLLDLIQEGNMGLLRAVHKFDPERGFKFSTYATWWIRQAILRAIS
                     EQSRTIRLPAHIGEAMGRVAQVSQDLHQKLGRTPKAEEIGKELGIEGSRVREILRAAQ
                     TPVSLEAPVGDESEENQLRDFVRDETSAAPEEMAAQTLLKKEMSDVLDDVLTERERIV
                     LQLRFGLGNGHQYPLEKVGEHLGVTRERVRQIEAEALRKLRSPRLTERFRDFL"
     gene            complement(2139..2212)
                     /locus_tag="VF276_01190"
     tRNA            complement(2139..2212)
                     /locus_tag="VF276_01190"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2176..2178),aa:Glu,seq:ttc)
     gene            complement(2376..>2981)
                     /locus_tag="VF276_01195"
     CDS             complement(2376..>2981)
                     /locus_tag="VF276_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605504.1"
                     /translation="EHSQNTYWLNNCFTAADLQPHLIYGSVLDNQKAVTVKGTYLQNR
                     QYLEFYYRENYTVVEMEAGPYLDALYESTYPTRHPSGEHINFTRLPFDLGILHYASDT
                     PYTRGKNLGAASLAYLGMDSAYATVVAMARRIFTMELAQMAREQERSRPHREVPDGAA
                     PVPAGEPGLPTANGNSGNESLQPAGMETDTGGRRHHRRADR"
BASE COUNT          532 a          998 c          982 g          469 t
ORIGIN      
        1 ctgcgcatcc cgtccgatcc cacgcgcctc agcccgcccg gtcctgccgc ccaactggcc
       61 gccgcgctca atatcgtggc cggggacatc ctcgtctgga tcgatccgaa agcggggcgc
      121 atgaacgcaa gtgcgatccg cgccctcgcc ggaccgctgc tgcacgaccc cggcgtcctg
      181 ttaagcaagc ccttctggcc cgaacccgat agcgagggca cgggcgtgcc ggaggggcgg
      241 gtgccgaatg cgcgcttcgc cccgatccgg gtcacggatc tcctggcgat gtccgtaccc
      301 gaactggcgg cgctaccgct ctatagctgg ctacgcgcgt tctatccccg gctgggggcg
      361 gtcttgaacc ccctcggcag cacctttgcc gcccgcgtga gcctcctgcg cgatctcttg
      421 cccagcttgc aggccacgga ggatgcgctg ggtggcgacg gcgcgcgcag tgctacgatc
      481 agcccggcca cagcctttgc cgccgggatg ctgctggaaa cggccgcgct gcacagcagc
      541 cgcgccatcg cgcaggtgga aatggcgggc agccggcgcg gaccacgagg atcccaggtc
      601 gccaatcgcg atacccgccg gttgcgccag gtggccgaat tgctcgccct gtttgccacc
      661 cggcacgatg cgatccagcg ccaggccgcg ctgcaggaga tccgcgggcg catcctcaac
      721 gccacggcct gaaacggggc gggcctgttg gcatatctct tgcacagcac accggctaca
      781 gacagcagcg ccccctgcta cccgcagcgc cctgcagggc gcactaccgg cgaccctggg
      841 gctgccccgc tgccgaacgt aaaaggagag agggatcaga gatgccgaga ggacgtaaga
      901 gcagcgcgca gagttcccac cccccgcccg cggtcgacgt gagcggcatc ggtctggacc
      961 cggagggcga ggccgccgct aaagccgaac acgacagccc ggaaggcgag gccctcttcc
     1021 aagaattgaa gcgcatggaa gaggcggcgc acaccggcgc agacctggca accattgtcg
     1081 atggtgcgcc tcccaccgct gcggccgccg cccccgcgcc cccggtcgcc gtggtcgccg
     1141 aggaggacga agaggaggag gagtcggaac cctgggtcga tgaagaagag gcgctacgcc
     1201 atatcgaccc ggcgtatgta accgacccgg tgcgcctcta tttacgcgag atcagccggg
     1261 ccccgctctt gagcagccag caggagttcg cgctggcccg cgccatcaac gaaggcgatg
     1321 tcgaagccac gcagaagttc gtgctggcaa acctgcgcct ggtcgtcagc atcgccaaga
     1381 agtacgtggg ccgcggcttg accctgctcg acctgatcca ggaaggcaac atgggcctgc
     1441 tgcgtgccgt gcataagttc gacccggagc gcggcttcaa gttctccacc tatgcgacct
     1501 ggtggatccg ccaggccatc ctgcgcgcca tctcggaaca gtcgcgcacc atccgcctgc
     1561 ccgcccatat cggcgaggcg atgggccgcg tcgcccaggt ctcgcaggac ctgcaccaga
     1621 agctgggccg cacgcccaaa gccgaagaga ttggcaagga gttgggcatc gagggcagcc
     1681 gcgtgcgcga gatcctacgc gccgcgcaga cgccggtgtc gctggaagcg ccggtgggcg
     1741 acgagtccga ggagaaccag cttcgcgact ttgtgcgcga cgagaccagc gccgcgcccg
     1801 aggagatggc cgcgcaaacg ctgctgaaaa aggaaatgtc cgatgtgctc gacgacgtgc
     1861 tgaccgagcg cgaacgcatt gtgctgcaac tgcgcttcgg cctgggcaac gggcaccagt
     1921 acccgctgga gaaagtaggc gagcacctgg gcgtcacccg cgagcgcgtc cgccagatcg
     1981 aggccgaggc gctgcgcaaa ctgcgctccc cccgtctcac cgagcgcttc cgcgacttcc
     2041 tctagaggat cacacgcgat caaaaaggcc gccagcgaaa cactggcggc cttttttaca
     2101 ctcagcactc agcactcagt actcagcact cggctaggta gcgcgtaggg gattcgaacc
     2161 cctgatctca tccttgaaag ggatgcgtcc taggccacta gacgaacgcg ccatgcaaac
     2221 tgtctaatta taccagaagc ccccccggcg cgcaaggact ttagactgag tctcaggacg
     2281 attacgccta aattagtagt tccccaccgg gattctgagg aggcaccggg ccggtttcaa
     2341 accggcccct acaacgttac agcgaagttg agcgattacc ggtcggcccg ccggtggtgg
     2401 cgccggcccc cggtgtcggt ctccattcca gcgggttgga gcgattcgtt gccgctgttg
     2461 ccgttggcgg tcggcaggcc cggctcgccg gccggcaccg gggcggcgcc atcgggcacc
     2521 tcgcgatggg gccggctgcg ctcctgttcg cgggccatct gggccagttc catggtgaag
     2581 atgcgccggg ccatggcgac cacagtggca taggccgagt ccatgcccag gtaggccagg
     2641 ctggccgcgc ccaggttctt gccccgtgtg tacggcgtgt ccgaggcata gtgcaagatg
     2701 cccaggtcga acggcagccg ggtgaagttg atatgctcgc cggaggggtg gcgggtgggg
     2761 taggtgcttt cgtacagcgc gtccaggtag gggccggcct ccatctcgac caccgtgtag
     2821 ttctcacggt agtagaattc gagatactgg cggttttgca ggtacgtgcc cttcaccgtg
     2881 accgccttct gattgtcgag caccgagccg tagatcaagt gcggctgaag gtcggcggcg
     2941 gtgaagcagt tgttgagcca gtaggtattc tgcgagtgct c
//
LOCUS       DASUYC010000075         2680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_819029,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000075 DASUYC010000000
VERSION     DASUYC010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819029"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VF276_01200"
     CDS             complement(<1..577)
                     /locus_tag="VF276_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2181 domain-containing protein"
                     /protein_id="HEX6605505.1"
                     /translation="MHQLLSYFAPRYGVTRLSEITWGHAVNTQARLARACASAAMMLE
                     CDVRASPAGEIILAHPPDTTSDLTFAGLLDAVRPLDKGIKLDFKDPAVGPACLEALRA
                     APLVQPVLLNADIVQGQGAGPPAVDGAAFIAACTRRYPRGFLSLGWTTTAPPAGGYTA
                     ANVRAMLEQCAGRAGIVFPVRANLLPGSWAAL"
     gene            782..2506
                     /locus_tag="VF276_01205"
     CDS             782..2506
                     /locus_tag="VF276_01205"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HEX6605506.1"
                     /translation="MLKRVHWLLALCMVACLGLAGVPAARAQSATTTVTLLHFSDYHS
                     HAVPFYSEGAPNQGGIARGIAYLKAQRQSTPNLLILDGGDMVNKGSPTWSDEYMCAEW
                     PWLNGLVDAMALGNHEFDYGQDVFKACQAQITYPIISANYVGADGKPLLTADGKTYIV
                     KEVGGVKIGLFAVAGTDFSTLVTAANRAPGATFSDRIAVARQIVDTLRNTEKVNAVVM
                     FGHELREDDYALAQAVPGIDLILGTHSHYKSELTTIPNTKTYFISPFQYLTYISNVQL
                     TFTGGQLTGVSGHLVKMDAQQPEDPQIAAQVTKMQADLEAKHPDRFKVLGTAAVELGV
                     DNVSDDETGLGNWSMEVVRAAAGVNAFFSTSSSFRASIPPGPITVETFYTAIPYKNSI
                     VTADMTGQQVANLLNVVVSKRGTDSFCQESGVRFQMQNGQATNIEILRDPANPAAGYA
                     PLDLSKTYKIGATNFMTGVSAFYKPVFAAASNVTDTKVDIGTLLTSTIQSAGTITAAL
                     DGRMGTAVPGGGGTTPGMPRTGNGQAPDGAGLALLAALGLLAAGLLVRARLRVPASLP
                     VSERTPRE"
BASE COUNT          477 a          939 c          862 g          402 t
ORIGIN      
        1 gcagcgccgc ccaggaaccg ggcaacaggt tcgcgcgcac ggggaagacg atcccggcgc
       61 gcccagcgca ctgttcgagc atggcgcgga cgttggccgc agtgtatccg cccgcgggcg
      121 gcgccgtggt cgtccaaccc agcgacagga agccgcgtgg atagcgccgc gtgcaggcgg
      181 cgataaaggc ggcgccgtcc accgcgggcg ggcccgcgcc ctgcccctgc acaatatcgg
      241 cgttgagcag cacgggttgg accaggggcg ccgcccgcag cgcctccagg cacgccggcc
      301 cgacagccgg gtctttgaaa tcgagcttga tccccttgtc cagcggccgc acggcatcga
      361 gcagccccgc gaaggtcaga tcgctggtgg tgtccggcgg gtgggccagg atgatctcgc
      421 ccgcgggcga cgcgcgtaca tcgcactcca gcatcatggc ggcggacgcg caggcgcggg
      481 ccagccgggc ctgtgtgttc acagcatgtc cccaggtgat ttcgctcagc cgggtcacgc
      541 cgtagcgcgg cgcgaagtac gagagtagtt ggtgcaatgt gctatactcc ctggggccgg
      601 catgggcggt gccccggtat gcgacgacgt taccgggcga gccggaccat tggcccggaa
      661 tcccggcggc ggcccatatc atcgcccggc gttgttagtt gtataatagc gcatcgctcc
      721 ccgcactgtt cccgcggccc gccattaccg gcacatctta atagttactt caggaggtca
      781 tatgttgaag cgagttcact ggctcctggc tctttgtatg gtcgcgtgcc tcggcctggc
      841 cggcgtgcct gccgcccgcg cgcaatcggc taccactacc gtcacccttc tgcactttag
      901 cgactatcac tcccacgccg tgcccttcta ttccgaaggc gcgcccaacc agggcgggat
      961 cgcccgcggc atcgcctacc tcaaggcgca gcggcagagc acgcccaact tgctgatcct
     1021 ggacggcggc gacatggtca acaaaggcag ccccacctgg agcgacgaat acatgtgcgc
     1081 cgaatggccc tggctcaacg ggctggtcga cgccatggcc ctcggcaacc acgagttcga
     1141 ttacgggcag gacgtgttca aggcgtgcca ggcccagatc acctacccca tcatcagcgc
     1201 caactacgtg ggcgccgacg gcaagccgct gctgacggcg gacggcaaga cctatatcgt
     1261 caaggaggtc ggcggcgtca agatcggcct gttcgcggtg gccggcaccg atttcagcac
     1321 cctcgtgacc gccgccaacc gcgcgccggg tgccaccttc agcgaccgca tcgccgtggc
     1381 ccggcagatc gtggacaccc tgcgcaacac cgagaaagtc aacgccgtgg tcatgttcgg
     1441 gcacgagttg cgcgaggacg actacgcgct ggcccaggcc gtccccggca tcgacctgat
     1501 cctgggcacc cactcgcact acaagagcga actgaccacg attcccaaca ccaagaccta
     1561 tttcatctcg ccgttccagt acctgaccta tatctccaac gtgcaactca ccttcaccgg
     1621 cggccagctg accggcgtga gcgggcacct ggtgaagatg gacgcccagc agcccgaaga
     1681 cccgcagatc gcggcccagg tcaccaagat gcaggccgac ctggaggcca agcaccccga
     1741 ccgcttcaag gtgctgggca cggcggcggt cgagcttggc gtggacaacg tgtcggacga
     1801 cgagacgggc ctgggcaact ggtcgatgga agtggtgcgc gcggcggcgg gggtcaacgc
     1861 cttcttctcc acgtcgtcgt cgttccgcgc ctcgatcccg ccgggcccga tcaccgtgga
     1921 gaccttctac acggccatcc cctacaagaa cagcatcgtc accgccgaca tgaccgggca
     1981 gcaggtagcc aatctgctga acgtggtggt ctccaagcgc ggcaccgaca gcttctgcca
     2041 ggagagcggc gtccgcttcc agatgcagaa cgggcaggcc accaatattg aaatcttgcg
     2101 cgacccggcc aacccggcgg cgggctatgc gccgctcgac ctgagcaaga cgtacaagat
     2161 cggcgccacc aacttcatga cgggcgtctc ggcgttttat aagccggtgt tcgccgcggc
     2221 gagcaacgtg accgacacga aagtggacat cggcacactg ctgaccagca cgatccagtc
     2281 cgcgggcacg atcacggcgg cgctcgacgg gcgcatgggc acggccgtgc cgggcggtgg
     2341 cggcaccacg ccgggcatgc ctcgcacggg caacggccag gcgccggacg gcgcaggact
     2401 cgcgctgctg gcggccctgg ggctgctggc cgccggcctg ctcgtccgcg cccgcctgcg
     2461 cgtgccggcg tccctgcccg tatccgagcg gacgccgcgc gagtaacagc gggacgccgt
     2521 agcgcatcgc gcccggtccc gtcattctga gcgcagcgaa gaatctccgt ctcgcaggcc
     2581 aaacgacgag actcttcgct ccgctcagag tgacaatagc gtagggaccg gcagcgaggc
     2641 aacgaccaca tcccccttgt cattctgcgc gcagcgaaga
//
LOCUS       DASUYC010000076         2666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_309399,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000076 DASUYC010000000
VERSION     DASUYC010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309399"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2666
                     /locus_tag="VF276_01210"
     CDS             <1..>2666
                     /locus_tag="VF276_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006104162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6605507.1"
                     /translation="HAYVQGATALRPTLVENERRIDADFTALAAADRQAGATLQTTPR
                     FQALRESWAELKAIPLDAQARVDRHTQFITDIRTLISQVGDSADLVLDPQLDSYYLAN
                     SLLVQLPDWQDLLAQATLLGEDMATQGAPPAQDRTQLIALRGLILADTTKARRNMNMA
                     FDSNPAGTLQPALQPPLEAAAAATEDFLQQIKQDLIDPPVVQSPAQPYRVAGAPARAA
                     TFVYWDAAIDRLDGLLQARLDEFGTRRWLVVGLTAAALLLVTYLWAGFYLAVMRTVSR
                     LDAAAQRMISGTAAEPVNLDNRDELGQVARAFNQLATALVSAGRYRQAVVDNAADAII
                     TTDSWGMIRSFNPAAEHIFGYQAGEVIGQPFTMLLETAAHAGPGARREVGGQRKDGSI
                     FPMDLSVSAMRLGDEELSICVLRDVTERKRAEDDLEQARDAAEAASRAKSEFLANMSH
                     EIRTPMNAVMGMTGLLLDTPLTDEQRDFAETIRRSSDDLLTIINDILDFSKIEADRLE
                     LENQPFDLRECLESSLDLLAPRAGEKGLELAYQIDAQVPNTLVGDVTRLRQILVNLIS
                     NAVKFTERGEIVVSVDAARQTDGRYELHFAVKDTGIGIPADRMDRLFRSFSQIDASTT
                     RRYGGTGLGLAISRRLSELMGGRMWVESTVGQGSTFHFTLLAAAGTSARRVHLQGTQP
                     QLEGKCLLIVDDNETNRRILTLQLQAWGMQAVEAASGPAALACLQEQDPFDGAILDLH
                     MPEMDGITLAGEIRKLPEGAQLPLIMLTSLGRREQEAEAAGFAAYLHKPMKPAQLYTV
                     LVEILGGQAPRKRAPGEAPQIDRQLAARLPLRLLVAEDNAVNQKLALQLLARMGYRAD
                     VAGNGLEVLQALERQVYDIVLLDVQMPEMDGL"
BASE COUNT          442 a          922 c          904 g          398 t
ORIGIN      
        1 ccatgcctat gtgcagggcg cgacggcgct gcgcccgacg ctggtggaga acgagcgccg
       61 gatcgacgcc gatttcacgg cgctcgccgc cgccgaccgg caggccggcg ctaccctgca
      121 gaccacgcct cgcttccagg cgctgcgcga gagttgggcc gagctgaaag cgatcccgct
      181 ggacgcgcag gcgcgcgtgg accgccatac ccagttcatc accgatatcc gcacgctgat
      241 aagccaggtg ggcgactccg ccgacctggt gctcgatccg cagctcgata gctactacct
      301 ggcgaactcg ctgctggtgc aactgcccga ctggcaggat ctgctggcgc aggcgaccct
      361 gctgggcgag gatatggcga cacagggcgc cccgccggcc caggaccgca cgcaactgat
      421 cgcgttgcgc ggcctcatcc tggccgacac cacgaaggcc cgccgcaaca tgaacatggc
      481 cttcgacagt aaccccgcgg gcacgctgca gcccgccttg cagccgccgc tggaagctgc
      541 cgccgcggcc accgaggact tcctgcagca gatcaaacag gatctgatcg acccgccggt
      601 cgtgcagtcc ccggcgcagc cctaccgcgt ggccggcgcc ccggcgcggg ccgccacctt
      661 tgtttactgg gatgccgcga tcgacaggct ggacggcctg ttgcaggccc ggctggacga
      721 gttcggcacc cgccgctggc tggtcgtagg gttgacggcg gcggcgctgc tgctggtgac
      781 ctacctgtgg gcgggtttct acctggccgt catgcgcacc gtctcccggc tcgatgccgc
      841 tgctcagcgc atgatcagcg gcaccgcggc ggagccggtg aacctggaca accgcgatga
      901 actgggccag gtggcgcgcg ccttcaacca gctcgccacc gcgctcgtct ccgccggccg
      961 gtaccgccag gcggtggttg ataacgccgc cgacgccatc atcacgaccg attcctgggg
     1021 catgatccgc tctttcaacc cggcggcgga acacatcttc ggctatcagg cgggcgaagt
     1081 gatcgggcag ccgtttacca tgctgctgga gacggccgcg cacgccgggc cgggcgcgcg
     1141 ccgcgaggtg ggcggccagc gcaaggacgg gtcgattttc cccatggacc tctcggtcag
     1201 cgcgatgcgc ctgggcgacg aggagctgag catctgcgtg ctgcgcgatg tgaccgagcg
     1261 caaacgggcc gaggatgacc tggaacaggc ccgcgatgcc gccgaggccg cctcgcgggc
     1321 caagtccgag ttcctcgcga acatgagcca cgagatccgc acgccgatga acgccgtcat
     1381 gggcatgacc ggcctgctgc tcgacacccc gctcacggat gaacagcgcg atttcgccga
     1441 gacgatccgc cgcagcagcg acgacctgct gacgatcatc aacgatattc tcgacttctc
     1501 taagatcgag gccgataggc tcgaactgga aaaccagccg ttcgacctgc gcgagtgcct
     1561 ggagtcctcg ctcgatctgc tggcgccgcg ggcgggcgag aaggggctgg aactggccta
     1621 tcagatcgac gcccaggtgc cgaacacgct ggtgggcgat gtgacccgcc tgcggcagat
     1681 cctggtcaac ctgatcagca acgccgtgaa gttcaccgag cggggcgaga tcgtcgtctc
     1741 cgtcgatgct gcccgccaga ccgatggccg ctatgaactg cacttcgccg tcaaggacac
     1801 cggcatcggc atcccggcgg accgcatgga tcgcctgttc cgctcgttca gccagatcga
     1861 cgcctcgacc acgcgccgct acggcggcac cgggctgggg ctggcgatca gccgccggct
     1921 gagcgagttg atgggcgggc ggatgtgggt cgagagcacg gtgggccagg ggtccacctt
     1981 ccacttcacc ttgctggccg ccgccgggac cagtgcccgc cgcgtccatc tccagggcac
     2041 ccagccgcag ttggagggca aatgcctgct cattgtggac gataacgaaa cgaaccgccg
     2101 catcttgacc ttgcaattac aagcctgggg catgcaggcc gtggaggccg cctcggggcc
     2161 ggcggcgctg gcctgtctgc aggagcagga ccccttcgac ggcgcgattc tcgacctgca
     2221 catgccggag atggacggca tcacgctggc cggggagatc cgcaaactgc ccgagggcgc
     2281 gcagcttccg ctgatcatgc tcacgtcgct gggccgccgg gagcaggagg ccgaggccgc
     2341 cgggttcgcc gcctacctgc acaagccgat gaagcccgcg cagctttata ccgtgctcgt
     2401 cgagatcctg ggcgggcagg cgccgcgcaa acgcgcgccg ggcgaagccc cgcagatcga
     2461 ccggcagctt gccgcgcggc tgccgctgcg cctgctggtg gccgaggata acgccgtgaa
     2521 ccagaaactg gcgctgcaac tgctggcgcg catgggctac cgcgccgacg tggcgggcaa
     2581 cggcctggag gtgctgcaag cgctcgaacg ccaggtctac gacattgtgc tgctcgacgt
     2641 gcagatgccc gagatggacg ggttgg
//
LOCUS       DASUYC010000077         2842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1926167, whole genome shotgun sequence.
ACCESSION   DASUYC010000077 DASUYC010000000
VERSION     DASUYC010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1926167"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..685)
                     /locus_tag="VF276_01215"
     CDS             complement(50..685)
                     /locus_tag="VF276_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605508.1"
                     /translation="MRPPRIRKALDDYAARQLPGAPDLWPAVRREVLRRHPATGRIHI
                     EPLGRRRGAWLPALGVLLLLAVLALGPWLNQGHVPVPAATPSPAPVAVPGAPTMPPAS
                     LPPGTDSGYVADLLARNLGTRLGMTQPQLDAALTAAVLDTIDQAVRDGQLTPAQAAQA
                     KVRAGQGLAALFRDGFAGAGSDLSSNGVRALLDALGPAPGAPTPTAPGSAK"
     gene            complement(663..1271)
                     /locus_tag="VF276_01220"
     CDS             complement(663..1271)
                     /locus_tag="VF276_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX6605509.1"
                     /translation="MTPDRDEQEAVARLQRGDISGLEVLVRRYQAPALRAAVLVCRDA
                     ALAEDLVQAAFVRAYERIGQFDARRPFGPWFIRGVVNDALKAVTRHRQVPLDPAALET
                     PAPPGDDPEARLAAAETREAIWAVLDELTPPQRAAIVLRYYADLSDAEIAQMLDCPPG
                     TVRRRMHDARRRLRRLLPAWLRPPGLDLAAAKGDADAPATDP"
     gene            complement(1650..>2842)
                     /locus_tag="VF276_01225"
     CDS             complement(1650..>2842)
                     /locus_tag="VF276_01225"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX6605510.1"
                     /translation="DAGLRTLTAVYHLQTAHNWTEFRAALADFDGPAQNFVYADRQGN
                     IGYQATGRIPIRKQGDGSVPVDGASGAYGWTGFIPFDQLPSAYNPPQGYLVTANNRIV
                     GAGYPYLLATRWTPGYRAARITQLLEALPRVSPDDSRRIQFDVQSAYAAEVGKYFAAL
                     PTTDARLAPVVRRFAGWDGALPGDSTLASIYEVAFTHLVSTTTANVLGTPLNRTYMDK
                     AWDEAKLFTLAALAEPDAAWWDDPATPARENRDAVLQTSLTAALDELAATLGDDPAGW
                     QWGKLHQIGFPHQLGGVPPLDKLFNLGPYPSAGDNLTVNSSYFQPTVSYTQLAHPSTR
                     QVTDVGNWDAMTVIIAPGQSGQPFARHWGDLTADWLAGRARPLPFSPAAVQAAAVDTL
                     TLQP"
BASE COUNT          388 a          943 c         1100 g          411 t
ORIGIN      
        1 caaggggcgt tcccacccgg cccgcgccgg gggccgcgcc gccagggtgc tatttcgcgc
       61 tgcccggcgc ggtgggggtt ggcgcgcccg gtgccggccc cagggcatcc aacagtgcgc
      121 gcaccccatt ggagctgaga tcggagccgg ctccggcgaa cccgtcgcgg aacagcgcgg
      181 ccaggccctg gccggcgcgg actttggcct gggcggcctg cgccggggtc agttgcccgt
      241 cgcggaccgc ctgatcaatc gtgtcgagga cggcggcggt gagcgccgcg tcgagttggg
      301 gctgggtcat gcccagccgg gtacccaggt tgcgggccag taaatcggca acatagccgc
      361 tgtcggtgcc gggcggaagc gacgcgggcg gcatggtggg cgcgcccggc acggccaccg
      421 gcgcgggtga cggcgtggcg gcgggcaccg gcacgtggcc ctggttgagc cacggcccca
      481 gcgcgagtac cgccagcagc agcaggacgc ccagcgccgg cagccacgcg ccccgacggc
      541 gacccagcgg ctcgatatgg atgcgcccgg tggcagggtg ccgccgcagc acctcgcggc
      601 gcaccgcggg ccacagatcg ggcgcgccgg gcagttgccg cgcggcgtaa tcgtccagcg
      661 ctttacggat ccgtggcggg cgcatcggcg tctcctttcg cggcggccag gtcgagtcct
      721 ggcgggcgca gccacgcggg caacagccgg cgcaggcgcc ggcgggcatc gtgcatccgg
      781 cggcgcaccg tgcccggcgg gcaatcgagc atctgggcga tctcggcgtc gctcagatcg
      841 gcgtagtagc gcaacacaat ggccgcccgc tgaggcgggg tgagttcgtc gagcacggcc
      901 cagatggctt cgcgggtttc cgcggcggcc agccgcgcct ccgggtcgtc ccccggtggg
      961 gcgggcgtct ccagtgctgc cgggtcgagc ggcacctggc ggtggcgggt gacggctttg
     1021 agggcgtcgt tgaccacgcc gcggatgaac cagggaccga acgggcggcg ggcgtcgaac
     1081 tggccgatgc gctcgtaggc gcgcacaaag gccgcctgca ccaggtcttc ggccagcgcg
     1141 gcatcgcggc aaaccagcac cgcggcccgc agcgccggcg cctgatagcg ccgcaccagc
     1201 acctccaggc cgctgatgtc gccccgttgc agccgggcca cggcctcctg ttcgtccctg
     1261 tctggcgtca tcgttccccc gcccgcggcg tgttgcttcc accggtacta tcccgcgcgg
     1321 ccccgatttg ttcggagcgg cgggcccgga ttcgggacca atatagcacg gtggcggagg
     1381 tggcggggcg ggcggatggg ctgccgtcgg gcgccacagc acaaggccgg tgtagggacc
     1441 ggtttgaaac cggcccgtgc gggaatgagg atctacgggt agggcatgga gaatccgttg
     1501 tcatgctgag cgcagcgaag catctcgtct tggtcccgca cgagacgaga ctcttcgctg
     1561 cgctcagcgt gacaagctcg ggggcacggc gcgagtctga gcgccgcgcg aaatgacacg
     1621 gaacagggcc gggtgaaacg accggggcct tacggctgca gggtcagcgt gtccacggcg
     1681 gcggcctgga ccgcggcggg gctgaagggt aacgggcgcg cccgcccggc caaccagtcg
     1741 gcggtgaggt caccccagtg gcgggcgaag ggctggcccg actggcccgg cgcgatgatc
     1801 accgtcatgg cgtcccagtt gcccacgtcg gtgacctggc gcgtcgacgg gtgggccaac
     1861 tgcgtgtagc tcaccgtggg ctggaagtag gacgagttca cggtcaggtt gtccccggcg
     1921 gaggggtagg ggccgaggtt gaacagctta tcgagcggcg gcacgccgcc caactggtgc
     1981 gggaagccga tctggtgcag cttgccccac tgccagcccg ccgggtcgtc gcccagcgtg
     2041 gccgcaagtt cgtccagcgc cgcggtcaag ctggtctgca gcacggcgtc gcggttctcg
     2101 cgggccgggg tggccgggtc gtcccaccag gccgcgtccg gctcggccag cgccgccagg
     2161 gtgaacagct tggcttcgtc ccaggctttg tccatatagg tgcggttgag cggcgtgccc
     2221 agcacattgg ccgtggtcgt gctgacgagg tgcgtgaagg cgacttcata aatcgaagcc
     2281 agggtgctgt cgccgggcag ggcgccgtcc catcccgcga accggcgcac cacgggtgcc
     2341 agtcgggcgt cggtggtggg cagcgcggca aagtacttgc ccacttcggc ggcataggcg
     2401 ctctgcacgt cgaactggat gcgccgcgag tcgtccgggc tgaccctggg cagcgcctcc
     2461 agcagttggg tgatacgggc ggcgcggtag ccgggggtcc agcgcgtcgc cagcaggtag
     2521 gggtagccgg cgccgacgat gcggttgttg gccgtgacca gatagccctg cgggggatta
     2581 taggcgctcg gcaactggtc gaaggggatg aatccggtcc agccataggc gccgctcgcg
     2641 ccgtccaccg gcacgctgcc gtcgccctgc ttgcggatcg ggatgcggcc tgtcgcctgg
     2701 tagccgatgt tgccctgcct gtcggcatag acgaagtttt gcgcggggcc gtcgaagtcg
     2761 gccagcgcgg cgcggaactc ggtccagttg tgcgcggttt ggagatgata cacggcggtg
     2821 agcgtgcgca gcccggcgtc cg
//
LOCUS       DASUYC010000078         3189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_865971,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000078 DASUYC010000000
VERSION     DASUYC010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865971"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VF276_01230"
     CDS             complement(<1..504)
                     /locus_tag="VF276_01230"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6605511.1"
                     /translation="MSDPITLMQQLGFSEYEARAYVALLERSPLNGYELAKESGLPRA
                     NVYAVLQKLEERAAVVRVDTPSGTRFAPVPPDELTRRLGSHFEDVLSAARGSLGAVRK
                     PAPHAQVWNAEGYAALLERARAIIGAAHEQLLLATWPEEAHALSAAVSGAVARGVSVS
                     TLCLRACP"
     gene            complement(563..1747)
                     /locus_tag="VF276_01235"
     CDS             complement(563..1747)
                     /locus_tag="VF276_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006889802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6605512.1"
                     /translation="MTHYDYLLIGGGMTGAAAIEGIRAVDKSGTIGLIGAEADRPYNR
                     PPLSKGLWKGDTTLDDIWTGAAESDATLHLGRTARAIDPQAKQVTDDTGTVYSYDKLL
                     LATGGTPRRLPFGGNDIIYYRTLGDYRRLRALAETGQRFAVIGGGFIGSEIAAALSMF
                     GKQVVLLFPEPAIGARIYPADLAQFLNDYYREHGVDVQAGASVTGVTPKGNGYRVHTK
                     EGQTFDVDGVVAGIGMQPNVDLAQAAGLRVDNGILVDEYLRTSAPAIYAAGDAARYPD
                     ALFGSRRVEHENNAVQMGKRAGRAMAGDEKPYRHQPFFYSDLFDLGYEAVGEMDSRME
                     TWADWKEPFREGVVYYLRDGRVRGVLLWNVWEQVGAARKLLAETGPFTAHDLQGRLPV
                     QQ"
     gene            complement(1744..2616)
                     /locus_tag="VF276_01240"
     CDS             complement(1744..2616)
                     /locus_tag="VF276_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6605513.1"
                     /translation="MYFGIYAANAETCADPQTTAQVAREAEAAGWDGFFLADNLLFER
                     VVPVADTWITLAAIAAQTERIRLGPLVTALPRRHLGRLARETVTLDHLSRGRLVLGVG
                     SGDDRWGEYSTFGPAPSQAQLGAMLDEALAVLTGLWSGQPFQFEGTYYHIGPTQFQPT
                     PVQQPGIPIWVAGRWPHKRPFRRAAAWNGVFPNAIDGELTPGDYRAIRAFLAEHGADL
                     THFDIVHTHEAVPLAHPADDMLRAYAQAGVTWWLVSFPDATPLAVLRDYIRGGPPRLP
                     AGEPLQQTQGGMQP"
     gene            complement(2637..>3189)
                     /locus_tag="VF276_01245"
     CDS             complement(2637..>3189)
                     /locus_tag="VF276_01245"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF013398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HEX6605514.1"
                     /translation="YMAEIFTAFGMETGTPATADTPARYIRALFDATSGYEGDPKLLT
                     VFSTECHGGPDCRLSQVIEGPIPFYALCEHHALPFYGQAYVGYIAHEGIIGISKLTRL
                     VRLYARRFSVQERLGRQIADTLVTLLAPHGVAVYLEAHHLCTQMRGVQETAAVTRTTF
                     WRGEYEQNPDLRAEFFTASGLPR"
BASE COUNT          462 a         1134 c         1096 g          497 t
ORIGIN      
        1 cgggcaggcg cgcaggcaga gcgtgctcac actcaccccc cgcgccaccg ccccgctgac
       61 ggccgcgctc agcgcgtgcg cctcctcggg ccaggtcgcc agcagcaact gctcgtgggc
      121 cgcgccgatg atcgcccgcg cgcgctccag caacgccgcg tagccctcgg cgttccagac
      181 ctgggcgtgc ggcgcgggct tgcgcaccgc gcccaacgac ccgcgcgccg cgctcaggac
      241 atcctcgaaa tggctgccca gccgccgggt tagctcgtcg gggggcaccg gcgcgaaccg
      301 ggtccccgac ggcgtgtcca cccgtaccac ggcggcgcgc tcctccagct tttgcagcac
      361 cgcgtagacg ttggcgcgcg gcaagcccga ctccttcgcc agttcgtagc cgttgagcgg
      421 gctgcgttcg agcagcgcca cgtaggcgcg ggcttcgtat tcgctgaacc ccaactgctg
      481 catcaaggtg atcggatcgc tcatcggtgt ctccttgtgg ccgcgctcgc cggctatacc
      541 cacgcccata ggccgcaggg cgttattgct gtaccggcaa gcggccttgc agatcgtgcg
      601 cggtgaacgg gccggtttcg gccagcagct tgcgcgccgc gcccacctgc tcccagacat
      661 tccacagcag taccccgcgc acgcgcccgt cgcgcagata gtagaccacg ccctcgcgga
      721 acggctcctt ccagtcggcc catgtctcca tccgcgagtc catctcgccg acggcctcat
      781 agccgaggtc gaacaggtcg gaatagaaga agggctggtg gcgatagggc ttctcgtcgc
      841 cggccatggc ccgcccggcg cgcttgccca tctgcacggc gttgttctcg tgctcaaccc
      901 gccgcgagcc gaagagggcg tcggggtagc gggcggcgtc gcccgccgcg tagatggccg
      961 gggcgctcgt ccgcaggtac tcgtcgacca ggatgccgtt gtccacgcgc aacccggcag
     1021 cctgggccag atcgacgttg ggctgcatcc cgatcccggc gaccacgcca tccacgtcga
     1081 atgtctgccc ctccttggta tgcacgcgat agccgttacc cttgggcgtc acgccggtca
     1141 cgctggcgcc tgcctggaca tcgaccccgt gctcgcggta gtagtcgttc aggaactgcg
     1201 ccaggtcggc cgggtagatg cgcgcgccga tggccggctc cgggaacagc agcaccacct
     1261 gcttgccgaa catactcagc gcggcggcga tctcgctgcc gatgaagccg ccgccgatga
     1321 ccgcgaaccg ctgcccggtc tcggccagcg cccgcagacg gcgataatcg cccagcgtgc
     1381 ggtagtagat gatgtcgttg ccgccgaagg gcagccggcg cggggtgccg cccgtggcca
     1441 gcagcagctt gtcgtagctg tagaccgtcc cggtgtcgtc cgtgacctgc ttggcctggg
     1501 ggtcgatcgc gcgggcggtg cggcccaggt gcagcgtggc gtccgattct gccgccccgg
     1561 tccagatgtc gtccagcgtg gtgtcgccct tccacagccc cttggacagc ggcgggcggt
     1621 tgtagggccg gtcggcctcc gcgccgatca gcccgatggt gccgctcttg tccacggcgc
     1681 ggatgccctc gatcgccgcg gcgcccgtca tgccgccgcc gataagcaga taatcgtaat
     1741 gggtcatggt tgcatacctc cttgtgtctg ctgaagtggc tcgcctgccg gcagccgggg
     1801 cggaccgccg cggatatagt cgcgcaggac cgcgagcggc gtagcgtcgg ggaacgacac
     1861 tagccaccac gttacccccg cctgcgcgta ggcgcgcagc atatcgtccg cggggtgggc
     1921 aagcggcacc gcctcgtgcg tatgcacgat atcgaagtgc gtcagatcgg cgccatgctc
     1981 ggccaggaag gcgcggatgg cgcggtagtc gccgggcgtc aattcgccgt cgatggcgtt
     2041 gggaaagacg ccgttccagg cggcggcgcg ccggaacggg cgcttgtgcg gccagcgccc
     2101 cgccacccag atcgggatgc ccggctgctg gaccggcgtg ggctggaact gggtggggcc
     2161 gatatggtag taggtgccct cgaattggaa gggctgcccg ctccacagcc cggtcagcac
     2221 ggccagggct tcgtccagca tcgcgccaag ctgggcctgg gagggggccg ggccaaaggt
     2281 gctgtactcg ccccagcggt cgtcgccgct gcctaccccc agcaccagcc gcccccgcga
     2341 cagatgatcc agcgtcacgg tctcgcgcgc cagccggccc aggtggcggc ggggcagcgc
     2401 cgtcaccagc ggccccagcc ggatgcgctc ggtctgcgcg gcgatggcgg ccaacgtgat
     2461 ccaggtatcg gccaccggca ccacccgctc gaacagcagg ttgtccgcga ggaagaaccc
     2521 gtcccagccg gcggcttccg cctcgcgggc cacctgcgcc gtcgtctgcg ggtccgcgca
     2581 cgtttcggca ttcgccgcgt agattccaaa gtacatgtcg gaccccccgc gtcgccctag
     2641 cgcggtagcc cgctagcggt gaagaactcg gcgcgcaggt ccgggttctg ctcgtactct
     2701 ccgcgccaga acgttgtccg cgtcacggcg gcggtttcct gcacgccgcg catctgcgta
     2761 cacaggtgat gggcctccag gtagacggcc acgccgtgcg gcgccagcag cgtcaccagg
     2821 gtatcggcga tctgccggcc caggcgctcc tgcacactga agcggcgggc atacaggcgc
     2881 accagccggg tcagcttcga gatgccgatg atgccctcgt gcgcgatata gccgacgtag
     2941 gcctgaccgt agaagggcag ggcgtggtgc tcgcagagcg catagaacgg gatcggtccc
     3001 tcgatcacct ggctgagccg gcaatcgggg ccgccgtggc attctgtgga gaagaccgtg
     3061 agcagcttgg ggtcgccctc gtagccgctc gtagcgtcga acagggcgcg gatgtagcgg
     3121 gccggcgtat ccgccgtcgc cggggtgccg gtctccatgc cgaacgcggt gaagatctcg
     3181 gccatatag
//
LOCUS       DASUYC010000079         2736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1820009, whole genome shotgun sequence.
ACCESSION   DASUYC010000079 DASUYC010000000
VERSION     DASUYC010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1820009"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VF276_01250"
     CDS             complement(<1..397)
                     /locus_tag="VF276_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEX6605515.1"
                     /translation="MATQTPEQFITARQADWARLEALSTRATRGRLSELTPTEIEELG
                     RLYRQASSDLARARRDYPADRLTLYLNRLVATSYTAIYREGGWSWRRIARFYSTDFPR
                     LVRKQWVYVAIAAALLFLPAIAAWIAVVVQ"
     gene            555..1418
                     /locus_tag="VF276_01255"
     CDS             555..1418
                     /locus_tag="VF276_01255"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX6605516.1"
                     /translation="MQTTDEQFVVATPELVEFGYEVAGVGSRFIAALVDTALIAVIYL
                     LIQVAGLATSYGGQSLFSKEGNSLSAIFLAVLTVLAFVVLWGYYVFFETLWNGQTPGK
                     RLTHLRVVREDSSPISLWEALIRNLVRLIDFLPLAYGIGVTVMFLNGRARRLGDFAAG
                     TLVVREGSAVTLQGLLARTAIAQTSPYGPPAPDSPLAAELDMRRLTPADLYLAREVLA
                     RQATLAPPAAWELCLKATQIIAARGGLRGTVTDPRAFLMEVLRLADRNAAPPAPDPAP
                     AAIGMQGPPPP"
     gene            1474..1568
                     /locus_tag="VF276_01260"
     tRNA            1474..1568
                     /locus_tag="VF276_01260"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1508..1510,aa:Sec,seq:tca)
     gene            1859..>2736
                     /locus_tag="VF276_01265"
     CDS             1859..>2736
                     /locus_tag="VF276_01265"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6605517.1"
                     /translation="MPAPLVDSLTQRVPPDQAVAEASLRLDAALAALVPFVPAPVRDI
                     HLAQDTPTRIPGLYLTGTVLSADLSGFTALSSELAAAGRQGSEEISALINRLFAVLVE
                     EVEAHGGGVIQFGGDGLTAVFDAARLGGTHPALASAAALAMQARMDAFSAVPTSKGPF
                     RLQVRLAVHTGKLFAAEVGDADHTELIVTGRVINRVELAQESAAPGEVIITANTLEAL
                     GHAATERRATGLYLLRALDHLPPLPAAARPAWQPGPPSPALIRALVHRIQACRPFVPH
                     GLPDRFLGADAEGGEFR"
BASE COUNT          387 a         1027 c          914 g          408 t
ORIGIN      
        1 gctggaccac gacggcgatc caggcggcaa tcgcgggcag gaacagcagc gccgccgcga
       61 tggcgacata gacccactgc ttgcgcacca gccgggggaa gtcggtgctg tagaagcgcg
      121 caatgcggcg ccagctccag ccgccctcgc ggtagatagc cgtgtagctg gtcgccacca
      181 ggcgattgag gtagagggta agccggtccg ccgggtagtc gcggcgggcg cgcgccaggt
      241 cggacgacgc ctggcgatag aggcggccca gctcctcgat ctcggtgggg gtcaactcgc
      301 tcaggcgccc gcgggtggcg cgcgtgctca gcgcctccag ccgcgcccaa tcggcctgcc
      361 gggcggtgat aaattgctcg ggggtttgcg tcgccatgcg cccattgtag gcagcccgcc
      421 ccgccacgtc aaggagacgg cgaacacggt ttatgcggct tgcggagaag ccgggcattc
      481 ggatagaata ggcgtagccg gcacctgccg ggcgcgtcgc gttatcccca cccggttgga
      541 aggaccgctc ctttatgcag accaccgacg agcaattcgt cgtcgccacg cccgaactgg
      601 tcgaattcgg ctacgaagtc gccggagtcg gctcgcgctt catcgccgcc ctggtcgata
      661 cggccctgat cgccgtgatc tatctgctga tccaggtggc cgggctggcg acctcctacg
      721 gcgggcagag cctgttcagc aaggaaggca acagcctcag cgccatcttc ctggccgtgc
      781 tgaccgtgct ggccttcgtc gtgctctggg gctactacgt cttcttcgag acgctctgga
      841 acggccagac ccccggcaag cgcctgaccc acctgcgcgt ggtgcgcgag gacagcagcc
      901 cgatcagcct ctgggaggcg cttatccgca acctggtgcg gctcatcgac ttcctgccgc
      961 tggcctacgg catcggcgtc acggtgatgt tcctcaacgg gcgcgcccgc cgcctgggcg
     1021 acttcgccgc gggcaccctg gtggtgcgcg agggcagcgc ggtgacgctg caaggcttgc
     1081 tggcccgcac cgccatcgcg caaacgtcgc cgtacggccc gcccgcgccc gactcgccgc
     1141 tggccgccga actcgacatg cgccgcctga cgcccgccga tctctacctg gcgcgcgagg
     1201 tgctggcccg ccaggccaca ctcgcgccgc ccgccgcctg ggaattgtgc ctgaaggcca
     1261 cgcagatcat cgccgcgcgg ggcggcctgc gcggcaccgt caccgacccg cgcgccttcc
     1321 tcatggaagt cttgcgcctc gccgatcgca acgccgcccc gcccgctccc gatcccgcgc
     1381 ccgccgccat cggtatgcaa ggcccgcccc cgccttgacg ttcgcgcctg tgttcaggta
     1441 tgctacaggc acgagacgcc gcccggcgtc cgggggggtg gttgggtccc ggtgggctcc
     1501 gcagtcttca aaactgtctg ggcgtcgcta agaacggcgt cggtgggttc gactcccatg
     1561 cactcccgcc ctgccccgcg cacggcccgc cgccagatta ctggcggcgg gcatctcgtt
     1621 atcgcgggcc ggcatccatg gcccaaagat tgcaaggtga tccccccata acgccgcgag
     1681 atgcgctaca atacgcgcgt gccgcgcgca cgacggaccg gcgtctggcc gccagggcgg
     1741 cgagttggca gacgccgccc ttccgatctc ctacatcctg ccgcgcgcag gacccggcgg
     1801 tggcttcttg ctgacagcag actggtctcc aacggacttt gggttactgc aaagcttatt
     1861 gcccgccccc ctggtcgatt cgctgaccca acgggtgccg cccgatcagg ccgtagccga
     1921 ggcgagcctg cgcctggacg ccgcgctggc cgcgctggtg ccgttcgtgc ccgcgcccgt
     1981 gcgcgacatc cacctggccc aggacacccc cacccgcatc cccggcctct acctgaccgg
     2041 cacggtgctc agcgccgacc tgtcgggctt caccgcgcta tcctcggaac tggccgccgc
     2101 cgggcgccag ggcagcgagg agatcagcgc gctgatcaac cgcctcttcg ccgtgctggt
     2161 ggaagaggtg gaagcccacg gcggcggcgt catccagttc ggcggcgacg gcctgaccgc
     2221 cgttttcgac gccgcccggc tcggcggcac gcacccggcc ctggcaagcg ccgccgccct
     2281 ggcgatgcag gcgcgcatgg acgccttcag cgccgtgccc accagcaagg ggccgttccg
     2341 gctgcaagtg cgcctcgccg tccacaccgg taagctgttc gcggcggaag tgggcgacgc
     2401 cgatcacacc gagctgatcg tcaccgggcg ggtaatcaac cgggtcgaac tggcgcagga
     2461 gagcgccgcg ccgggcgaag tgatcatcac ggccaatacg ctcgaagccc tgggccacgc
     2521 ggccaccgag cggcgggcca cgggcctcta cctgctgcgg gcgctcgacc acctgccgcc
     2581 cctgcccgcc gccgcccggc ccgcctggca gccggggccg ccgagtcccg cgctgatccg
     2641 ggcgctcgtg caccgcatcc aggcgtgccg gcccttcgtg ccccacggcc tgcccgaccg
     2701 cttcctgggc gccgacgctg agggcggcga gttccg
//
LOCUS       DASUYC010000080         2036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_77020,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000080 DASUYC010000000
VERSION     DASUYC010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77020"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(400..603)
                     /locus_tag="VF276_01270"
     CDS             complement(400..603)
                     /locus_tag="VF276_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010390049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605518.1"
                     /translation="MDQQPNKPAPANNGMAGLGAGIAIGVGVGTALGVALGSMTLGVA
                     VGTAIGIALGVAFSRQQQPPRDH"
     gene            complement(794..1651)
                     /locus_tag="VF276_01275"
     CDS             complement(794..1651)
                     /locus_tag="VF276_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007460262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6605519.1"
                     /translation="MQYGLELSNADFAGDARTLADLAALAEATGWDGVFLEDYIVHWS
                     GPAAPTYDPWVALATMAVNTRRVRLGTTVTPLARRRPWKVARETVTLDHLSGGRLTLG
                     VGLGDVSDPGFGAVGEETGGKVRAARLDEALDVLAGLWSGVPFSYQGTHFQVHEVTFQ
                     PRPLQTPRIPIWVGGNWPNKGPVARAARWDGFVGGKVHAPDADWHLTPAEVRALKADI
                     QRYRTSAAPFDIALGGGEPVFELEQERALIRAMAEAGASWWMEFVPTSLGSLEAMRAR
                     IARGPIRID"
     gene            complement(1781..>2036)
                     /locus_tag="VF276_01280"
     CDS             complement(1781..>2036)
                     /locus_tag="VF276_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605520.1"
                     /translation="AGGVVRGGPQWRFYSVVLFLAGLYNIILGILTVFFRLTQADTAT
                     ALLQTFGGLILCLISAFTLSAAATAERTPDFDRQGTYEEY"
BASE COUNT          319 a          669 c          746 g          302 t
ORIGIN      
        1 cggtggtggt gacggtgatc gtggcgctgg gcgcggcgcg gctcgcggac aaccccagca
       61 gcgacaccac cagggtaagc accaggaaga gcgaccgcac gcctacccgc gccggccatt
      121 tcgcagaacg gaccatgatc atctcctcct cgatggtgga tggttacgtt gtacccaccg
      181 ccggctcacg cgccgccagg gacaaggtgc gagaaggtta agtcgtgtcg agtggaacgc
      241 aggcgaggac taccggtggc cgccggtcgc actaggagta caccagatta cccttagcgt
      301 agcaccgatt ccaggcggtg tcaacggcac acagatggtg cgtttcttta caattacgta
      361 aggatttggt aagggggcgg atcttacggc ggcgggcgcc tagtgatccc tggggggctg
      421 ctgctgacgg ctgaaggcca cgccgagggc gatgccgatg gcggtgccca cggccacgcc
      481 gagcgtcatg ctgccgaggg cgacgccgag cgccgtgccc acgccgacgc cgatggcgat
      541 gccggcgccg aggcccgcca tgccgttgtt ggcgggggcc ggtttgttgg gttgctgatc
      601 cattagttaa gtgctccttc cgtcaggcga ccgcgccggg tggcggcgcc gggttcccgc
      661 attatacccg aaccccgccc gccggtccag ggcgggcggc gcatccattc cgtcgttcac
      721 cgcgaagaaa cgaaggcaac gaagattatt aagattggtc ttgtgcttcg cggcgaactg
      781 cgaaacgccg gggctagtcg atgcggatgg ggccgcgggc gatacgggcg cgcatggcct
      841 ccaggctgcc cagcgaggtg ggcacgaact ccatccacca ggaggccccc gcctcggcca
      901 tggcgcggat cagagcgcgc tcctgctcca actcaaagac cggctcaccg ccgcccagcg
      961 cgatgtcgaa cggggcagcg ctggtccgat agcgctggat gtcggctttc agggcgcgca
     1021 cctcggcggg ggtgagatgc cagtcggcgt cgggggcgtg gactttgccg ccgacgaagc
     1081 cgtcccagcg cgcggcgcgg gcgaccgggc ctttgttcgg ccagttgccg cccacccaga
     1141 tcgggatgcg cggcgtctgc agcgggcgcg gctggaaggt gacttcgtgg acctggaagt
     1201 gggtgccctg gtagctgaag gggacgccgc tccagagacc ggccagcaca tcgagcgcct
     1261 cgtcgagccg cgcggcgcgc accttgccgc cggtctcctc gccgaccgcg ccgaagccgg
     1321 ggtcgctcac gtcgcccagg cccacgccga gcgtcaggcg cccgcccgac aggtggtcga
     1381 gcgtgacggt ttcgcgggcc accttccagg gccgccgccg ggccaggggc gtgacggtgg
     1441 tgcccaggcg cacgcgccgg gtgttcaccg ccatcgtggc cagggctacc caggggtcgt
     1501 aggtgggggc ggcggggccg ctccagtgga cgatgtagtc ttcgagaaag acgccgtccc
     1561 agccggtggc ctcggccagc gcggccaggt cggccagcgt gcgcgcatcc ccggcgaagt
     1621 cggcgttcga gagttcgagt ccgtactgca tgcgccctcc cgttcgccct ggtccatcag
     1681 cagccgggcc ggtcccggcg cgggccggtt tgaaaccggc ccctacaccg cacggtttcg
     1741 gtaggggccg gtttcaaact ggcctccgcc cgcgccccga tcaatactcc tcatacgtgc
     1801 cctggcggtc gaagtcgggc gtccgctcgg cggtggcggc ggcggagagg gtgaacgcgg
     1861 agatcaggca gaggatgagc ccgccgaagg tctgcaacag ggcggtggcg gtatcggcct
     1921 gggtgaggcg gaagaagacg gtgaggatgc ccaggatgat gttgtagagc ccggccagga
     1981 acaggaccac gctgtagaag cgccactgcg gcccgccgcg cacgacgccg cccgcc
//
LOCUS       DASUYC010000081         3238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_770675,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000081 DASUYC010000000
VERSION     DASUYC010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770675"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /locus_tag="VF276_01285"
     CDS             <1..1004
                     /locus_tag="VF276_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HEX6605521.1"
                     /translation="AGVRAGARQIECTINGIGERAGNASLEEVVMALHTRRPFFDLET
                     GIQTREISRASRLVAACTGVHVPPNKAIVGANAFAHEAGIHQDGILKNRLTYEIMSAE
                     TVGQDGSALVLGKHSGRHAFRQHLQELGYVLADDEFRRAFARFKELADKKKVIDDRDI
                     EALLAGEAGRPVSIYELDQVQVACGTHAIPTATVRMRGPDGAMHVESAQGTGPVDAVC
                     QAVNKIVGQPGELIEFAVNAVTEGIDAVGEVSIRVQEYGAHTNDTVPAENSLAPRPGR
                     PRVFTGYGVNTDIVVAAAEAYVSALNKLFHARQEAQPTAGAAVAPAAQAAPVAASYA"
     gene            1020..2615
                     /gene="cimA"
                     /locus_tag="VF276_01290"
     CDS             1020..2615
                     /gene="cimA"
                     /locus_tag="VF276_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HEX6605522.1"
                     /translation="MQILLYDTTLRDGTQREGLSLSAADKVKIARELDSLGVHYIEGG
                     WPGSNPKDAEFFVRIADVPLRNARVAAFGSTRHADVRCEDDANIQALVAANTPVVTMF
                     GKSSTLHVERVLETTREENLRMIADSVAYFKALGKEVVYDAEHFFDGYRLDADYALAT
                     IVAASAAGADWIVLCDTNGGALPDQVAETVRTARGRVQTPLGIHTHNDGALAVANAMA
                     AVDAGCRQVQGTINGYGERCGNMDLVPLIANLQLKRGYQCVAPEQLRRLSEVSQFVAA
                     VANLNPDTHAPYVGHSAFAHKGGVHVAAIAKVAESYQHIDPALVGNEMRVVVSELAGR
                     GNVRLRAAALGLPLNGNERAVLERIKELENDGFQFEAAEGSFEMLVRRSDPGYVPPFE
                     LLDFTVLVEKRGPNAVMSQATVKIRVGDEVMHTAAQGDGPVNALDRAARKALLPHYPE
                     LADIHLVDYKVRIVDEYLGTAARPRVLIESACGEERWSTVGCSENIIAASWQALADSL
                     ELPLLRRGATPRAAALAGAPAPA"
     gene            2637..>3238
                     /locus_tag="VF276_01295"
     CDS             2637..>3238
                     /locus_tag="VF276_01295"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEX6605523.1"
                     /translation="MPIPATKYIWFNGEMVPWEKATVHVLTHALHYGSSVFEGIRCYD
                     TPNGSALFRPAPHIRRLFDSAKVYRMELRFDAVQLLDACKRVVRENGLKSAYIRPLVW
                     RGYGEIAPSPLKNPVEVMIAAWNWGAYLGEDALENGADVAVSSWTRMAPNTIPAMAKA
                     AGNYLSSQLITMEAERNGYVEGIALDVNGQLSEGAGENLFV"
BASE COUNT          559 a         1103 c         1112 g          464 t
ORIGIN      
        1 tggcgggggt gcgcgcgggc gcccggcaga tcgaatgcac gatcaacggc atcggcgagc
       61 gggcgggcaa cgcctcgctc gaagaggtcg tcatggcgct gcacacgcgg cgcccgttct
      121 tcgacctgga gaccggtatc cagacccgcg aaatctcgcg ggcctcgcgc ctcgtggccg
      181 cctgcaccgg cgtgcatgtg ccgcccaaca aggccatcgt cggcgctaac gctttcgccc
      241 acgaggcggg catccaccag gacggcatcc tgaagaaccg cctgacctac gagatcatga
      301 gcgccgagac ggtgggccag gacggcagcg ccctggtgct cggcaagcac tctgggcgac
      361 acgccttccg ccagcacctg caggagctgg gctatgtgct ggccgacgac gagttccgcc
      421 gtgccttcgc ccgctttaag gaactggccg ataagaaaaa ggtgatcgac gaccgcgaca
      481 tcgaggcgct gctggccggg gaggccgggc gcccggtctc gatttatgag ttggaccagg
      541 tgcaggtggc ctgcggcacc cacgccatcc ccacggcgac cgtgcgtatg cgcggccccg
      601 acggcgcgat gcacgtcgag tcggcgcagg gcaccggccc ggtggacgcc gtctgccagg
      661 ccgtgaacaa gatcgtcggc cagccgggcg agttgatcga gttcgccgtc aacgccgtga
      721 ccgaagggat cgacgcggtg ggcgaggtga gcatccgcgt gcaggaatac ggcgcgcaca
      781 ccaacgacac cgtgcccgcc gagaacagcc tggcgccgcg gcccggtcgc ccgcgggtct
      841 ttaccggcta tggcgtgaac accgacatcg tggtggcggc ggcggaagcc tacgtcagcg
      901 cgctcaacaa gctgttccat gcgcgccagg aggcccagcc cacggcgggc gccgcggtag
      961 cacccgccgc ccaggccgcg ccggtcgcgg cgagctacgc ataagcagag gagtcgccga
     1021 tgcagatcct actctacgac acgacgctcc gtgacggcac gcagcgcgag ggcctgtcgc
     1081 tctccgccgc cgacaaggtc aagatcgccc gcgaactcga cagcctgggc gtccactaca
     1141 tcgagggcgg ctggccgggg tcgaacccca aggacgccga gttcttcgtg cgcatcgccg
     1201 atgtgccgct gcggaatgcc cgtgtcgccg cgttcggcag cacccgccat gccgatgtcc
     1261 gctgtgagga cgacgccaat atccaggcgc tcgtcgccgc caacacgccg gtcgtgacca
     1321 tgttcggcaa aagctccacg ctccatgtcg agcgggtgct ggagacgacg cgcgaggaaa
     1381 acctgcgcat gatcgccgac agcgtggcct acttcaaggc gctgggcaaa gaggtggtct
     1441 acgacgccga gcacttcttc gacggctacc ggctcgacgc ggactacgcc ctggcgacta
     1501 tcgtcgccgc gagcgccgcg ggcgccgact ggatcgtgct gtgcgacact aacggcggcg
     1561 cgctgcccga ccaggtagcc gagacggttc gcacggcgcg cgggagggtg cagacgccgc
     1621 tgggcatcca cacgcacaac gacggcgcgc tggccgtcgc caacgccatg gccgctgtgg
     1681 acgccgggtg ccgccaggtg cagggcacca tcaacggcta cggcgaacgc tgcggcaaca
     1741 tggacctggt gccgctgatc gccaacctgc aacttaagcg cggctaccag tgcgtggcgc
     1801 ccgagcagtt gcgccgcctc agcgaggtct cgcagttcgt ggccgccgtg gcgaacctga
     1861 accccgacac ccacgcgccc tacgtgggcc acagcgcctt cgcccacaag ggcggcgtgc
     1921 acgtggccgc catcgccaag gtggccgaga gctaccagca catcgacccc gcgctggtcg
     1981 gcaacgagat gcgcgtggtg gtgagcgaac tggccgggcg gggcaatgtg cgcctgcgcg
     2041 ccgccgcact gggcctgccg ctgaacggca acgagcgcgc ggtgctggag cgcatcaagg
     2101 agctggagaa cgatggcttc cagttcgagg ccgccgaggg gtctttcgag atgctggtgc
     2161 gccggtccga ccccggctat gtgccgccgt ttgaactgct cgacttcacc gtgctggtcg
     2221 agaagcgcgg cccgaacgcc gtgatgtcac aggccacggt caagatccgc gtgggcgacg
     2281 aggtgatgca caccgcggcc cagggcgacg gcccggtgaa cgcgctcgac cgtgccgcgc
     2341 gcaaggcgct gctgccccac taccccgaac tggccgacat ccacctggtg gactataaag
     2401 tccgtatcgt cgacgagtac ctgggcacgg cggcccgccc gcgcgtgctg atcgagtcgg
     2461 cgtgtggcga ggaacgctgg agcaccgtgg ggtgctcgga gaatatcatc gcggcaagct
     2521 ggcaggcgct ggccgatagt ctggagttgc cgctgttgcg tcgcggggcc acgccgcggg
     2581 cggcggccct ggccggggca ccggctccgg cataagacaa ctaaaggaga cctcgaatgc
     2641 ctattcctgc tactaaatat atctggttca acggcgagat ggtgccctgg gagaaggcga
     2701 ccgtccacgt gctgacccac gcgctgcatt atggttcctc ggtgttcgag ggcatccgct
     2761 gctatgacac gcccaatggg tcggccctgt tccgcccggc gccgcatatc cgccgcctgt
     2821 tcgactcggc caaagtctac cgcatggaac tgcgcttcga cgccgtgcaa ctgctggacg
     2881 cctgcaagcg cgtcgtgcgc gagaacggcc tcaagagcgc ctacatccgc ccgctggtct
     2941 ggcgcggcta cggcgagatc gccccatcgc cgctcaagaa cccggtcgag gtgatgatcg
     3001 cggcgtggaa ctggggcgcg tatctaggcg aagacgcgct ggagaacggc gccgatgtgg
     3061 ccgtgtcgtc ctggacgcgc atggcgccca acacgatccc cgccatggcc aaggccgccg
     3121 ggaactatct gtcctcgcaa ctgatcacca tggaagccga acgcaacggc tacgtcgagg
     3181 gcatcgcgct cgacgtaaac ggccagctta gcgagggcgc gggcgagaac ctgttcgt
//
LOCUS       DASUYC010000082         2694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_307397,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000082 DASUYC010000000
VERSION     DASUYC010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307397"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VF276_01300"
     CDS             complement(<1..252)
                     /locus_tag="VF276_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605524.1"
                     /translation="MEDILTADFAGAVAQYGDTMLFVGFDLGDGQLQITVYPGYRFPA
                     DDRVHGHIGIFDVPVGTPWTEIMQRVDAIEAQAQRNYQRC"
     gene            complement(285..686)
                     /locus_tag="VF276_01305"
     CDS             complement(285..686)
                     /locus_tag="VF276_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEX6605525.1"
                     /translation="MDRQSQIDNLLELYQHPEHRGTLEDADVSVKGGNPGCGDIVCYH
                     LKVGDDGRISALRFEGQGCTISQAAAEFVAERAEGASLESIEDLTHDEIIDLLGREVV
                     MSRPRCATLALGTLKDAVKTYRQGRRSVCPE"
     gene            1030..1614
                     /locus_tag="VF276_01310"
     CDS             1030..1614
                     /locus_tag="VF276_01310"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEX6605526.1"
                     /translation="MEFNVAQLMKEPVGAKRSYEFGTPELLLSEALPGESQTLVARDV
                     QGHITLTRLRAQVRAVGNVQAQVELQCSRCLESFVTAVEAPLDELFKQTIDVVSGHPM
                     PESDRPDEDEDDLFPISQNHILDLTEPVRQALLVALPMQPLHSEDCRGLCPTCGTNWN
                     LAQCDCAPDEGDPRFAALSNLLADFDTSDRHSNN"
     gene            1672..1869
                     /gene="rpmF"
                     /locus_tag="VF276_01315"
     CDS             1672..1869
                     /gene="rpmF"
                     /locus_tag="VF276_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX6605527.1"
                     /translation="MGAVPKQRISRAQQGKHRTGYRAKTIHVVECPNCHNLHRSHYVC
                     PSCGTYKGLQVYEIKQAKTEE"
     gene            1960..>2694
                     /locus_tag="VF276_01320"
     CDS             1960..>2694
                     /locus_tag="VF276_01320"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX6605528.1"
                     /translation="MKNYAAIRGWGMYTPERVMSNDEMATFVDTSDEWIRTRTGIAQR
                     HILADDETTSSLATHAARQALAQADLPADQLDLIIVATCTPDYLLPSTACLVQEALGA
                     KNAAAFDLGAVCSGFMYALVTATQFVQTGSYRNVLVVGAESLSRFLNFKDRTTCVLFG
                     DGAGAVVLQANESPAGVLSFVLNAQGSGADLIKIPAGGSAAPGMRGSLAEEDYYIHMS
                     GGEVYQFAVRTMSEDSVNAVRRAGLTL"
BASE COUNT          462 a          909 c          854 g          469 t
ORIGIN      
        1 gcaacgctgg tagttgcgct gggcctgggc ctcgatggcg tccacccgct gcatgatctc
       61 ggtccacggc gtgccgacgg gcacgtcgaa gatgccgata tggccgtgca cccggtcgtc
      121 ggcggggaag cgatagcccg gatagacggt gatctgcaac tggccgtcgc ccaggtcaaa
      181 gccgacgaac agcatcgtgt cgccgtactg ggccacggcc cccgcgaaat ccgcggtcag
      241 gatatcttcc atatcaggcg atccggtctg gatgggcgcg ggcgctattc gggacagaca
      301 gagcgccggc cctggcgata ggttttgacg gcgtccttga gtgtacccaa cgccagcgtg
      361 gcgcaccggg gccggctcat caccacttcg cgccccagca ggtcaatgat ttcgtcgtgc
      421 gtcaggtcct cgatggactc caggctggcg ccctcggccc gctcggccac gaactcggcg
      481 gcggcctggc tgatggtgca gccctggccc tcgaagcgca gcgcgctgat gcgcccgtcg
      541 tcgcccacct tgaggtgata gcagacgata tcgccgcagc cggggttgcc gcccttcacc
      601 gagacatcgg cgtcttccag cgtgccccgg tgttcggggt gctgatacag ttccaacagg
      661 ttatcgatct gggattgtcg atccatcata tgctcgtttg tttctctaca tgaatagggc
      721 caggcacgcc cggccccggc cagtctcact actataactt tacacgtatt gctgtgtaac
      781 ggggatctac gctgcattct agcacgcgcc ggggcgctgc gtcaacgcgc ccgcccaacc
      841 tctcccccaa cccctccccg ccacggggag gggagttcgc cgacgggcgt tccccccttc
      901 ccgcgccggg aaggggggca gggggggtta ggccagccgc gccgcgcttg acatccacgc
      961 ctgcgttccc tataatacag aggttatgcc gcgcccgatg cgcggatggg agttatgtgt
     1021 ctgatggaca tggaatttaa tgttgcccag ctcatgaaag agccggtcgg cgcaaagcgc
     1081 agctacgagt tcggcacgcc cgaattgctg ctcagcgagg cgctgccggg ggagtcgcag
     1141 acgctggtag cgcgggatgt gcagggccac atcacgctga cccggctgcg agcgcaggtg
     1201 cgcgccgtgg gcaacgtgca ggcccaggtc gaactccagt gcagccgctg cctggaatcc
     1261 tttgtgaccg ccgtcgaggc gccgctggac gaactgttca agcagaccat cgacgtggtc
     1321 agcggccacc ccatgcccga gagcgaccgc ccggacgagg acgaggacga cctgttcccc
     1381 atcagccaga accatattct cgatctgacc gagcccgtgc gccaggcgct gctggtcgcg
     1441 ctgcccatgc agcccttgca cagcgaggac tgccggggcc tctgccccac ctgtggcacc
     1501 aattggaacc tggcccagtg tgattgcgcc cccgacgagg gcgatccgcg ctttgcggcg
     1561 ctctcgaatt tgctggccga ttttgacacc agcgaccggc attctaacaa ctaggacacc
     1621 ctcgccgccc gcggcgaacc agcaacagta gcaaaggagt ttgacaaggt catgggagct
     1681 gtcccgaagc aacgaatctc gcgcgcgcag cagggtaagc atcgcaccgg ctaccgcgcg
     1741 aagacgattc acgtcgtcga gtgcccgaat tgccacaacc tgcaccgctc gcactatgtg
     1801 tgcccgtcgt gcggcaccta caagggcctg caagtgtacg aaatcaagca ggccaagacc
     1861 gaggagtaat cccggctgcc ccgcggcggc gccagctcca tccgaagctg ccgccgccgc
     1921 gagttctatc cgtgggcttc gtcattcagg agcatggttg tgaagaacta cgccgcaatt
     1981 cgcggatggg gcatgtacac gccggagcgc gtcatgtcca acgacgagat ggctactttt
     2041 gtcgatacct cggatgagtg gatccgcacg cgcaccggca tcgcccagcg ccacatcctc
     2101 gccgatgacg aaaccacctc ttccctcgcc acccacgccg cccgccaggc cctggcccag
     2161 gcggacctcc ccgccgacca gttggacctg atcatcgtcg ccacctgcac gcccgattat
     2221 ctcttgccct cgaccgcctg cctggtccag gaggcgctgg gcgccaagaa cgccgccgcc
     2281 ttcgacctgg gcgccgtctg ctcgggcttc atgtatgccc tggtcacggc gacccagttt
     2341 gtgcagaccg gcagttaccg caacgtgctg gtcgtcggcg ccgagtcgct gagccgcttc
     2401 ctgaacttca aggaccgcac cacctgtgtg ctgttcggcg acggggcggg cgcggtcgtg
     2461 ttgcaggcca acgagtcccc ggcgggcgtg ctctcgttcg tgctcaatgc ccaggggtcg
     2521 ggcgccgacc tgatcaagat cccggcgggt ggcagcgccg cgcccggcat gcgcgggtcg
     2581 ctggccgagg aagattatta tatccacatg agcggcggcg aggtctacca gttcgccgtg
     2641 cgcaccatga gcgaggattc ggtgaatgcc gtgcgccggg cggggttgac gctc
//
LOCUS       DASUYC010000083         3390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1924048, whole genome shotgun sequence.
ACCESSION   DASUYC010000083 DASUYC010000000
VERSION     DASUYC010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1924048"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(429..1094)
                     /locus_tag="VF276_01325"
     CDS             complement(429..1094)
                     /locus_tag="VF276_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013222893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6605529.1"
                     /translation="MDRVRILIADDHPVFRYGIRALLQAEPTTEVVGEATNGDEAVAA
                     AAALQPDLVLMDLNMPGLNGIEATRRILATRPQTGILVLTMFDDDDSVFAAMRAGARG
                     YLLKGAEGAETVGAIHIISSGEAIFSPAIARRLIQYFGASPPAMAPPAFPDLTDRERE
                     ILALIAEGYTNTAIAERLGLSSKTVRNHVSGIFGKLQVADRAEAISRARAAGLGPAQG
                     RSR"
     gene            complement(1130..3250)
                     /locus_tag="VF276_01330"
     CDS             complement(1130..3250)
                     /locus_tag="VF276_01330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX6605530.1"
                     /translation="MNPLLAPAAPQANPGRLAMALAWWSRHRFALAHAGSLALIGLAL
                     VYFLLALPAQFAYLQTVCDGGGCALTPAQAGELVALGLPAGFFAGYGIVVNVVVTLSW
                     FAVGGTIYRRRRDDPMALFTATMVITYGTITASVPVADLAGYPLLQGLFAVVAFWGNT
                     SSFLLFYLFPNGRFVPGATRYLAIPVVGIGVCLFFFPTWAVTQWLISWRFIQFVAILA
                     SGVFAQLYRYWRVSGPAQRQQTKWVVLGLTGAVVGTLGTQIALIASGPHGIRVSLVAD
                     TVVILAQLLIPLSIGAAILRYRLWDINILISRTLVYGALTAIVVGLYVLVVGALSALF
                     QASSSLLISLVATGLVAVLFQPLRERLQRSANRLLYGERDDPYALLSRLGQRLEATLA
                     PEALLPAIVETTGQALKLPYAAILVRRGDALNLAAAYGTPPDPATLVRLPLVSHSEPV
                     GELALAPRAPGESFAGADLRLLQHLAQQMAVAVHNVRLTADLQRLTADLQQSRERLVV
                     AREEERRRLRRDLHDGLGPQLASQTLTLSAARTLLRRDPDTAETLLADATAHAQQAIT
                     DIRRVIYALRPPALDDLGLVGVLQEQINQYRASGVTITLHAPEHLPPLPAAVEVACYR
                     IAQEALTNVVRHAHAHNCAIQISIDSALALEISDDGQGLPPAYQAGVGLNSMRERVAE
                     LSGTFGAEPGPNGGTRIRVRLPLS"
BASE COUNT          622 a         1128 c         1139 g          501 t
ORIGIN      
        1 caattgcaga tattggcata gtatgccgcc ttgctcccgg cgcagacgat tcgggccgga
       61 tggaagccga aactctatca cccaacgata tgataccgat cgcaggttaa aatcgcgttg
      121 gaatatttgc agaggtcttt aaggattccg gcgatgagca ggcatgtgta cgctgggcgg
      181 ccgccgcggt tgggcaggcg aacacctgcg gcgccagcgg cgaaccctgc cccggcctct
      241 acttccggcc caacgccaac gtgacccgcg ggcagaccag caagttcatc agcaacgcct
      301 tcttccccaa ctgccagacc ccgcgctaac cgcgcacccg accgaggcgc ccgcgcgggc
      361 accacggaac cagccaggcc gccgaggttc gccccggcgg cctggctgct ttcggcccgc
      421 tgtctcctct aacgggaccg gccctgcgcc ggtcccaacc cggcggcgcg ggcgcggctg
      481 atggcttcgg cccgatcggc gacctgtagc ttgccgaaga tcccggagac gtggttgcgc
      541 accgtcttgg aactgagtcc cagccgctcg gcgatagccg tgttggtgta gccttccgcg
      601 atcagagcca ggatctcccg ttcgcggtcg gtgagatccg ggaaagcggg cggggccatg
      661 gcaggcgggg atgcgccgaa atactggatc aggcgccggg cgatcgccgg gctgaagatc
      721 gcctccccgc tgctgatgat gtggatggcg cccaccgtct ccgcgccttc cgcgcctttg
      781 agcagatagc cgcgcgcccc ggcccgcatc gcggcgaaca cagaatcgtc gtcgtcgaac
      841 atcgtcagca cgaggatacc ggtctgcggg cgggtcgcca ggatgcggcg ggtcgcctcg
      901 atgccgttca ggccaggcat gttgagatcc atcaggacca ggtcgggttg cagtgctgcg
      961 gcagcggcga cggcttcgtc gccgttcgtc gcttcgccca cgacttccgt cgtcggctcg
     1021 gcttgcagca gagcgcggat accgtagcga aagacgggat gatcgtcggc aatcaagatg
     1081 cgaacacggt ccatagtaac gcaaaactcc tctattctca ggccaggaat caggacaacg
     1141 gcaggcggac ccgaatacgg gtgccgccat tggggccggg ttcggcccca aaggttccgc
     1201 tcagttccgc gacccgctcg cgcatcgagt taagccctac tccggcctgg taggcgggcg
     1261 gcagaccctg gccgtcgtcg ctgatttcca gcgccagcgc gctgtcgatg ctgatctgta
     1321 tcgcgcagtt gtgggcgtgg gcgtgacgca ccacattggt gagggcttct tgagcgatgc
     1381 gataacaggc gacctccaca gccgcgggta acgggggcag atgctccggc gcatggaggg
     1441 taatcgtgac gccactggcg cggtactggt ttatctgctc ctggagcacc cccaccaggc
     1501 ccaaatcgtc cagggccggt gggcgcagag cgtagatcac ccggcggata tcggtgatcg
     1561 cttgctgcgc gtgcgcggtg gcgtcggcca gtaaggtttc cgccgtgtcc gggtcgcggc
     1621 gcagcagcgt ccgcgcggcg gagagggtga gcgtctggct ggcaagctgc ggcccgagtc
     1681 cgtcgtgcaa atcgcggcgc aagcggcggc gctcttcctc gcgcgccacc accaaccgct
     1741 cgcgggactg ctgcaaatcg gccgtcagcc gttgcagatc ggcggtcagg cgcacattat
     1801 ggaccgccac ggccatttgc tgggcgagat gttgcagcag ccgcaagtcg gcccccgcga
     1861 agctttcgcc gggcgcccgc ggcgccagcg ccagttcgcc aaccggctcg ctatgcgaga
     1921 cgagcggcaa ccggaccagc gtggccgggt cgggcggcgt accataggca gccgccaggt
     1981 tgagcgcgtc gccccgccgg accaggatgg cggcataggg tagtttcagc gcctggccgg
     2041 tggtctcgac aatcgccggc aacagggcct ccggcgcgag ggtggcctcc aggcgctgcc
     2101 ccaaccggga cagcaacgca tagggatcat cgcgttcgcc atagagcagg cggttggcgc
     2161 tgcgctgcaa gcgttcgcgc aagggttgga acagcacggc caccagcccc gtcgccacga
     2221 gcgagatcag caggctgctg cttgcctgga acagcgccga cagcgcgccg accaccagca
     2281 cgtagaggcc aacgacgatg gcggtcagcg ccccgtagac cagcgtgcgg ctgatcagga
     2341 tattgatgtc ccacaagcgg tagcgcagga tggcggcgcc aatcgaaagc gggatcagca
     2401 actgcgccag gataaccacc gtgtctgcca ccagcgagac ccggatgccg tgcgggccgg
     2461 acgcgatcag cgcgatctgc gtgcccagcg tgcccaccac cgcgccggtg aggcccagca
     2521 ccacccactt ggtctgctgg cgctgcgccg gccccgatac acgccagtag cgatagagtt
     2581 gcgcgaacac cccggaggcc aggatggcga cgaactggat aaagcgccag gaaatcagcc
     2641 actgcgtaac cgcccaggta gggaagaaaa agagacagac gccgatcccg acgacgggga
     2701 tcgccaggta gcgggtcgcg ccgggcacaa accggccgtt gggaaacagg tagaacagca
     2761 ggaaggacga ggtatttccc cagaaagcga cgacggcgaa caacccctgt agcagcgggt
     2821 atcccgccag atcggccacc gggacggaag cggtgattgt accgtaggtt atcaccatcg
     2881 tggccgtaaa caacgccatg gggtcgtccc ggcgcctccg gtagatggtc ccgccgaccg
     2941 cgaaccagct cagagtcacg acaacattca ccacgatacc gtagccggcg aagaagcccg
     3001 cgggcaggcc gagcgccacg agttcgccgg cctgggcggg ggtcagcgcg catcccccgc
     3061 cgtcgcatac ggtttgcagg tacgcaaact gggcgggcag cgccaggagg aaataaacca
     3121 gcgccaggcc gatcagcgcc aggctccccg cgtgggccag cgcgaaccgg tgccggctcc
     3181 accaggcgag agccatggcg aggcggccgg gattggcctg cggcgccgcc ggggcaagta
     3241 gagggttcac tgccgccctc ctctccgcac cattgagtga ccaccgatgc ataaggttgt
     3301 gcccaaccgg ccaccggccg attctctgca ctctaccacg ttttggccgg cttcgctaga
     3361 cccgcgcccg gcccgaacgg agccgggata
//
LOCUS       DASUYC010000084         4330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_254288,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000084 DASUYC010000000
VERSION     DASUYC010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254288"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="VF276_01335"
     CDS             complement(<1..768)
                     /locus_tag="VF276_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEX6605531.1"
                     /translation="MADAAERADGAERTDYDAAARPSGGSGAPAGQFIDGTFSNEAGT
                     RRYKLYIPSGYDGQPVPLIGLLHGCTQPVEELAAGTRMNLLAEAQTFLVVYPQQSSHA
                     NRATCWNWWRTGDQQRGAGEPALIAGLTQELMARYRVDPRRVYLAGISAGGALTTIMG
                     ATYPDLYAAIGVHSGVAYGTAHSMLSALDVMRRGREGVALPATRRNGRAAVPPRFVPL
                     ILFQGDRDRVVHPSNADWVIDQWLDACKRLTGAHGVEL"
     gene            1115..2302
                     /gene="bktB"
                     /locus_tag="VF276_01340"
     CDS             1115..2302
                     /gene="bktB"
                     /locus_tag="VF276_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketothiolase BktB"
                     /protein_id="HEX6605532.1"
                     /translation="MAEEREIVVLSGVRTAVGKYGGSLKDFSPTDLGGMVIKEAVRRS
                     GLEPGDIGHVVFGNILHTDSGDMYGGRLAMLKAGLPIEVPALTLNRLCGSGLQAIVTA
                     SHYIKLGYTDAAVAGGMEVMSRAQYWLPGMRWGQRMNDATVIDSMVAVLTDPQDGCHM
                     GITAERVATKYNISREDQDALAVESHRRAVQAIDNGYFREQILPIEIKTKAGVTVFDT
                     DEGPRRDANTAAMAKLKPAFDKTGSVTAGNASSINDAASAVVLMERKAAEARGLRPLG
                     RLVADSVAAVDPKYMGIGPVPAVRALLERTGLTIKDMDVIELNEAFAAQALGVIRELD
                     LPMDRTNPNGSGISLGHPVGASGAILTVKALNELQRTGGRYALVTLCIGGGQGIAAIY
                     ERL"
     gene            2366..3109
                     /locus_tag="VF276_01345"
     CDS             2366..3109
                     /locus_tag="VF276_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP reductase"
                     /protein_id="HEX6605533.1"
                     /translation="MNRLEGKTAFVTGGSRGIGRAIALAFAAEGAKVAVVYHAHEEAA
                     VEVQKEIIRKGGEALVVQADIANSAQIRAAIQFVIEQWGRVDILVNDAAITHDKTLRK
                     MTDEDWREVIDTNLNGVFYATSAVIPSMIANQYGRIINISSYTGHYGNFGQANYGAAK
                     AGVIGLTKTAALELAKYQITVNAICPGYTETDMMATIPSNIREQLTARIPVGRFATPE
                     EIAWGAVFLAADTGFVTGQEIGINGGMGM"
     gene            3167..3736
                     /locus_tag="VF276_01350"
     CDS             3167..3736
                     /locus_tag="VF276_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605534.1"
                     /translation="MSQTTNDGTSLFDPFGIMGNLKQARDVQLEAWSKFMIDLVSSDE
                     YARATGRALAESLAVSQPARQALERSMTATLEALNMPSRAEVISIAERIVNVEMRLDD
                     LDAKLTAYQKALPAAVQGVVAAAVREAVQEVVQDAVKEAVRQALAPTTRHLRELDTRL
                     TALDTRIAALQQAVAPPAPATEKAQEGQP"
     gene            3733..>4330
                     /locus_tag="VF276_01355"
     CDS             3733..>4330
                     /locus_tag="VF276_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6605535.1"
                     /translation="MSTPKTTTDEAQSEIQRLWENRETLRHVLTTRYPVGQTPHEVVW
                     TLNKAKLIRYLPTHPSKRKHRTPFLLVFALMSRAYILDLRPGASFVEYMTNQGYDVYL
                     LDWGAPGYEDRNLKFDDYVLDYMPRAIRKMKEVSGEEEFSMLGWCIGAILATCYAALR
                     PNDGLRNLILLTAPLDFSNQNMGGFAKWVRGDYLDVESV"
BASE COUNT          821 a         1447 c         1384 g          678 t
ORIGIN      
        1 aagctccacc ccgtgcgcgc cggtcaggcg cttgcaggca tccagccact ggtcgatcac
       61 ccaatcggcg ttactcgggt ggaccaccct gtcgcgatcg ccctggaaca gaatgagcgg
      121 cacgaaacgc gggggcaccg ccgcgcggcc attgcggcgc gtggccggga gggccacgcc
      181 ttcccggccc cggcgcatca cgtccagcgc cgagagcatg ctgtgcgccg tgccataggc
      241 gaccccggaa tgtaccccga tcgcggcata gagatcggga taagtggcgc ccatgatggt
      301 ggtcagcgcg cctcccgcgg agatcccggc caggtacacc cgccggggat cgacccggta
      361 tcgcgccatc agctcctggg tcagcccggc gatcagcgcc ggttcacccg ccccgcgctg
      421 ttgatcaccg gtgcgccacc agttccagca cgtcgcgcga ttggcgtggc tcgactgctg
      481 gggatacacg accaggaagg tctgcgcctc ggccagcagg ttcatgcggg tgcccgcggc
      541 cagctcctcg accggctgcg tgcagccgtg caacagcccg atcagcggca cgggttgccc
      601 gtcatagccg ctggggatat acagtttgta ccggcgagtg ccggcctcgt tgctgaacgt
      661 gccgtcgata aactgccccg ccggtgcccc acttccgccg gagggacgcg ccgccgcgtc
      721 ataatccgtg cgttccgccc catctgcccg ctccgccgca tccgccattg gacaccttct
      781 cccttttccc cgattcggta agccgcgccc gcgcgccagg gcgcctgccg ccattgtgca
      841 ggccccgctc cggcaccatt tcaagatcca ggccgccgct ccgcgctgcc ggccgcgcaa
      901 gcggcagcgg ccccgccccg gcccctgtac acgtattgcc acccgtttgt aacctggcga
      961 tacccaatta tacacgcccg gctgctagac tgaccctcat agcgcggcaa aatggcccgg
     1021 cgtctgcgca ttacatcctt cgccgttgac aggcagacac cgttttgtgg agtctccgta
     1081 gttgctctgc atgaaaggaa caaggtggtt caggatggca gaagaacgag aaattgtcgt
     1141 actaagtgga gtccgcacag cggtcggcaa gtacggcggc agcctcaaag acttttcgcc
     1201 caccgacctg gggggcatgg tcatcaagga agctgtccgg cgatcggggt tggaaccggg
     1261 ggacatcggc catgtcgtgt tcggtaacat cctccatacc gatagcggag acatgtatgg
     1321 aggccgctta gccatgctca aggccggcct gcctatcgag gtcccggcgc tcaccctcaa
     1381 ccggctgtgc ggcagcggct tacaggccat cgtcaccgca tcgcactaca ttaaactggg
     1441 ctataccgac gcggctgtgg cgggtggcat ggaagtgatg agccgcgcgc agtactggct
     1501 gcccggtatg cgctggggcc agcgtatgaa cgacgccacc gtgatcgatt cgatggtcgc
     1561 cgtgctgacc gacccgcagg acggctgcca catgggcatc accgccgagc gcgtcgccac
     1621 caagtataac atctcccgtg aagaccagga cgcgctggcg gtcgagagcc atcgccgggc
     1681 ggtgcaggcg atcgacaacg gctacttccg tgagcagatc ctgcccatcg aaatcaagac
     1741 caaggccggg gtcaccgtgt tcgacaccga cgaagggccg cgtcgcgatg cgaacaccgc
     1801 ggcgatggcg aaactcaagc ccgcctttga caagaccggc agcgtcaccg ccggcaacgc
     1861 ttccagcatc aacgacgccg cctccgcggt ggtcctgatg gagcgcaaag ccgccgaggc
     1921 gcgcggcctg cggcccctcg gccggctcgt cgccgattcc gtcgcggccg tggatccgaa
     1981 atacatgggc atcggcccgg tcccggccgt gcgcgcgctg ctggagcgca ccgggctgac
     2041 gatcaaagac atggacgtga tcgagttgaa cgaggccttc gcggcgcagg cgctcggcgt
     2101 catccgcgaa ctcgacctgc cgatggatcg caccaatccc aacggcagcg gtatctcttt
     2161 gggccacccg gtcggcgcca gcggcgcgat cttgactgtg aaagccctga atgagttgca
     2221 gcgcacgggt gggcgctacg cactggtcac gctgtgcatc ggcggcggcc agggcatcgc
     2281 ggccatctac gagcgcctgt aaagaccggg ccgccccgtg cgacccacgg ggcgcgccgc
     2341 aagccctagt cggaggaaca aagccatgaa tcggttggaa ggcaagaccg cttttgtgac
     2401 gggcgggtcg cgcggcattg gccgggccat tgccctggct ttcgcggcgg aaggcgccaa
     2461 ggtggccgtc gtctatcacg cgcatgaaga ggcggcggtc gaggtgcaaa aagagatcat
     2521 ccgcaagggt ggcgaggcgc tggtcgtgca agccgacatc gctaacagcg cgcaaatccg
     2581 cgccgccatc cagtttgtga tcgagcagtg gggccgggtc gacatcctgg tgaacgatgc
     2641 cgcgatcacc catgacaaga ccctgcgcaa gatgaccgat gaggattggc gcgaggtgat
     2701 cgacaccaac ctcaacgggg tgttctacgc cacctcggcg gtgatcccgt caatgatcgc
     2761 taaccagtat gggcggatca tcaacatcag ttcctacacc gggcactacg gcaacttcgg
     2821 gcaggccaac tatggcgccg cgaaggccgg cgtgatcggc ctgaccaaga ccgccgccct
     2881 tgaactggcg aagtaccaga ttaccgtcaa cgcgatttgc cccggctaca cggaaaccga
     2941 catgatggcg acgatcccga gcaacatccg tgagcaactc acggcgcgca tccccgtggg
     3001 ccgtttcgcc acgcccgaag agattgcgtg gggcgcggtc ttcctcgccg ccgataccgg
     3061 ctttgtgacc ggccaggaaa tcggcatcaa cggcggcatg ggcatgtaac catcgctggc
     3121 cggcgaccac gctgcacccc cgcgtagcga gggggaagga attacgatga gccagactac
     3181 aaatgatggc accagtctgt tcgatccctt cgggatcatg ggcaatttga agcaggcccg
     3241 cgacgtccaa ctggaagcct ggtcgaagtt catgatcgac ctggtcagtt ccgacgagta
     3301 tgcacgggcc accggacgcg ccctggccga aagcctggcg gtgtcgcagc ccgcccggca
     3361 ggcgctggag cgatcgatga ccgcgacgct ggaggccctg aatatgccga gccgggccga
     3421 ggtgatcagc atcgccgagc ggattgtcaa cgtggaaatg cgcctcgacg acctggacgc
     3481 caagctcacg gcctaccaga aagcgctgcc cgcggcagtg cagggggtcg tggcggcggc
     3541 ggtgcgcgaa gccgtgcagg aggtagtcca ggacgcggtg aaggaagcgg tgcgccaggc
     3601 cctggcgccc accacgcgcc atctgcgcga actcgacacg cgcctgacgg cgctcgatac
     3661 ccgaattgcg gcgttgcagc aagcggtggc cccgccggcg cccgcgacgg agaaagcgca
     3721 ggagggccag ccatgagcac gccaaaaacc accaccgacg aagcgcaatc cgaaattcag
     3781 cggctctggg agaatcggga aacgctgcgc cacgtgctaa ccacccgcta cccggtgggc
     3841 cagacccccc atgaggtcgt ctggacgctc aacaaggcca agctgatccg ctatctcccg
     3901 acccacccga gcaagcgcaa gcaccgcacg ccgttcttgc tggtcttcgc gctgatgagc
     3961 cgggcctaca tcctcgatct gcgccccggc gccagtttcg tcgaatatat gacgaaccag
     4021 ggctacgatg tctacctgct ggattggggc gcgcccggct acgaagaccg caacctcaag
     4081 ttcgacgatt acgtgctgga ctatatgccc cgcgcgatcc gcaagatgaa ggaagtatcg
     4141 ggcgaagaag agttcagtat gctcggctgg tgcatcgggg ccatcctggc gacctgctat
     4201 gccgccctgc gccccaacga cggcctgcgg aacctcatcc tgttgaccgc gccgctggac
     4261 ttcagcaacc agaacatggg cggcttcgcc aagtgggtgc gcggcgacta tctcgacgtt
     4321 gagagcgtca
//
LOCUS       DASUYC010000085         3198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1938390, whole genome shotgun sequence.
ACCESSION   DASUYC010000085 DASUYC010000000
VERSION     DASUYC010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1938390"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1924)
                     /locus_tag="VF276_01360"
     CDS             complement(<1..1924)
                     /locus_tag="VF276_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX6605536.1"
                     /translation="MDDSTPPSDLTPPAKKPRAPRKRSSAPVEPIAPVESVAEDAPLP
                     PPELVPVPGETDAVPPRKRRTTRKAVAAAPADEAPAPPTDEVPAPEPPARLEPVAAAP
                     VNVATDVPVAEPATPPKRRRVARKAVAAGPPPLSVPGPLGPIAKDFDPHDPAQLIAAF
                     RARYPFPLDAFQEEAIGFLAANESVLVAAPTGTGKTVVAEFGVFRAHARGWRIIYTTP
                     IKALSNQKFRDLREQYGDAVGLLTGDIVENREGAILIMTTEVLRNMLLQTPSELWGVG
                     CIVFDEIHYLADKERGTTWEESIIMCPPDVQLACLSATVSNAGEIAEWISRTHRPVHL
                     VSHTQRAVPLSYYYYLDGELNLTINAAGVQVANFAGVGGEVRRRVRGSAWSSAGDEDD
                     GPARERPEPTPREIVESLERRNMLPAIFFLFSRRDCEAAAEVCAMMRFRAAHEPATRR
                     RIETVIATYMDRLAPEDRTLEQVQSITYLARRGLGFHHAGLLPILKQLVEELFSAGLM
                     RVVFATDTLALGINMPARSVVIGRMSKWDGVQRRPLLPNEFQQMAGRAGRRGIDEQGH
                     VVIPYSPWVSFEDALAIATGPLLPVESAFTVRYNTILNLWDPPDGDRVLDVMRHSLLQ
                     FQQSRRLLELEDTVAEW"
     gene            complement(1994..2965)
                     /locus_tag="VF276_01365"
     CDS             complement(1994..2965)
                     /locus_tag="VF276_01365"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEX6605537.1"
                     /translation="MAGLNWVDGVLLVLVALVAWGATTRGFVQVFTGLVGFVLTLLAA
                     LLLTAPLAEWLGRNLAVGQLWAAPIAFLLIWLAAQIVFTAISRLILHRTHYQASRSTL
                     NRWLAVLPGAAQGLLVGGLLLTVLALAPIPGVPQQAILHSAIGGALVRTTVAFERPLE
                     GVFGPALRQTLGFLTVKPEPESGETVALKFQVPAPTVDADAEERMLMLINAERSARGL
                     APLAMDPALRQLARAHATDMFQQGYFSHTSQDGRSPFDRMHDARIYYSAAGENLALAP
                     TTEFAHDGLMNSPGHRANILNGQFHRVGIGVMDGGIYGKMFVQEFTD"
BASE COUNT          512 a         1072 c         1117 g          497 t
ORIGIN      
        1 cccactcggc caccgtgtct tccagctcca gcaaacgccg tgattgctgg aattgcagca
       61 ggctgtggcg catcacatcc agcacccggt cgccgtccgg cgggtcccat aggttgagga
      121 tggtgttgta gcgcacggtg aaggcgcttt ccaccggcag cagcgggccg gtggcgattg
      181 cgagcgcatc ctcgaaggag acccatgggc tataggggat gaccacgtgg ccctgctcgt
      241 cgatgccgcg ccgcccggcc cgcccggcca tctgctggaa ctcgttgggc aagagcgggc
      301 ggcgctgcac gccgtcccac ttggacatcc gcccgataac cacgctacgc gcgggcatat
      361 tgatgccgag cgccagtgtg tcggtggcga agaccacgcg catcagcccc gcgctgaaca
      421 actcctcgac cagttgcttg aggatcggca ggaggccggc gtggtggaag cccaggcccc
      481 gccgcgccag gtaggtgatc gactgcacct gctccagcgt gcggtcctcc ggcgccaggc
      541 ggtccatata ggtggcgatg acggtctcga tgcgccgccg cgtcgccggt tcgtgggccg
      601 cgcggaagcg catcatcgcg cagacctccg ccgccgcctc gcagtcgcgc cggctgaaga
      661 ggaagaagat cgccgggagc atgttgcgcc gctccagcga ttcgacgatc tcgcgcggcg
      721 tcggctcagg ccgttcgcgg gccgggccgt catcctcgtc gcccgcgctg ctccacgccg
      781 agccgcgtac ccggcggcgc acctccccgc ccacgcccgc gaagttggcc acctgcacgc
      841 ccgccgcgtt gatggtcagg ttcagttcgc cgtcgagata gtagtagtag ctcagcggca
      901 cggcgcgctg ggtgtgcgag accaggtgca ccgggcggtg cgtgcggctg atccactcgg
      961 cgatctcgcc ggcgttgctc accgtggcag acaggcaggc cagttgcacg tcgggcgggc
     1021 acatgatgat cgactcttcc caggtggtgc cgcgctcctt gtccgccagg tagtggatct
     1081 cgtcgaacac gatgcagccg acgccccaga gctcggacgg cgtttgcagc agcatgttac
     1141 gcagcacctc ggtggtcatg atcaggatcg cgccctcgcg attctcgacg atgtccccgg
     1201 tcagcaggcc cacggcgtcg ccatattgct cgcgcaggtc gcggaacttc tggttgctca
     1261 gcgccttgat cggcgtggtg tagatgatcc gccagccgcg ggcgtgggcg cggaagaccc
     1321 cgaactcggc caccactgtc ttgccggtgc cggtcggcgc ggcgaccagg accgattcgt
     1381 tggccgccag gaagccaatg gcctcctcct gaaaggcatc cagcggaaac gggtagcggg
     1441 cgcggaaagc ggcgatcagt tgcgcgggat cgtgcgggtc gaagtctttg gctatgggac
     1501 cgagcgggcc gggcacggat agcgggggcg ggccggcagc gacggctttg cgcgcgacgc
     1561 ggcggcgttt gggcggagtt gccggttcgg ccaccggcac atcggtcgcc acattgaccg
     1621 gcgcggcagc caccggttcc agcctagcgg gcggctccgg cgcgggcacc tcatctgtgg
     1681 gcggcgcggg cgcttcatcg gcaggcgcgg cagctaccgc cttgcgcgtc gtccggcgct
     1741 tgcggggcgg gaccgcatcg gtctcgccgg gcaccggcac cagttcgggc ggcggtaagg
     1801 gcgcgtcctc ggcgacagac tcgaccggcg ctatcggctc gacgggcgca gaggaacgct
     1861 tgcgcggggc acgaggcttc ttagcgggcg gcgtcaggtc ggatggcggg gtcgaatcgt
     1921 ccaagttggc atgctcctca atgtcggctg attgggccgt atggcacccg gctactgcac
     1981 aaatcaggcc gcgttagtcg gtgaactcct gcacgaacat cttgccgtag atgccgccgt
     2041 ccatgacgcc gatgcccacg cggtggaact ggccgttgag gatgttggcc cggtggccgg
     2101 ggctgttcat cagcccatcg tgcgcgaact cggtggtggg ggccagggcc aggttttcgc
     2161 ccgcagcgct gtagtaaatg cgggcatcat gcatgcggtc gaacggcgag cggccatcct
     2221 ggctggtgtg cgagaaataa ccctgctgga acatatcggt ggcgtgcgcc cgcgccaact
     2281 ggcgcagcgc cgggtccatg gcgagcgggg ccagcccccg cgcgctgcgc tcggcgttga
     2341 tgagcattaa catccgctcc tcggcatcgg cgtccacggt cggcgccggc acctggaact
     2401 tgagcgccac cgtctcgccg ctttcgggtt cgggcttgac ggtgaggaag ccgagcgtct
     2461 ggcgcagagc cgggccgaac acgccttcca gaggccgctc gaaggcgacc gtcgtgcgca
     2521 ccagtgcgcc gccgatggcc gagtgcagga tggcctgctg gggcacgccg ggaatgggcg
     2581 ccagggcaag cacggtcagc agcagcccgc ccaccagcag cccctgcgct gcgcccggca
     2641 gcacggccag ccagcggttc agcgtggacc gcgacgcctg gtagtgggtg cgatgcaaaa
     2701 tcagccggga gatggcggtg aagacgatct gggcggccag ccagatcagc agaaaggcga
     2761 tgggcgccgc ccagagctgg cccaccgcca ggttgcggcc cagccactcg gccagcggcg
     2821 ccgtcagcag cagggcggcg agcagcgtca gcacaaagcc caccagcccg gtaaacacct
     2881 gcacaaagcc gcgcgtcgtc gcgccccagg ccaccagcgc caccagcacc agcagcacac
     2941 cgtccaccca gttcagtccc gccacccgtt ccccctcgca tatcggccaa ccatcaggaa
     3001 agtatagtcg cgcggcgccc tggctgtcaa tcaggcaggc ctgacatctg ttcattcacg
     3061 ctattgtgca ccgcgaagac gcgaaggacg cgaagaagaa aactgatata aagaaccttc
     3121 gcgctcttcg ttgctttgcg gtgcatttat gaccggctgt gagggcgcgc ggtggagacg
     3181 accggccctt cgacgggc
//
LOCUS       DASUYC010000086         2952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1922513, whole genome shotgun sequence.
ACCESSION   DASUYC010000086 DASUYC010000000
VERSION     DASUYC010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1922513"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /locus_tag="VF276_01370"
     CDS             complement(<1..1145)
                     /locus_tag="VF276_01370"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEX6605538.1"
                     /translation="MSDTPSPTTRLERDSLGEVEVPADALYGVQTVRGIHNFPITGWL
                     PHPLLVEATVTVKKAAAITNQQLGQLDDQRAKAIVQAADEILAGRHREHFRIDPFQAG
                     AGTSHNMNTNEVLANRANEILGAPRGSYKPVHPNDHVNMAQSTNDVFPTSMRLAVLMN
                     LETFYPAITAMAEAYAEKGREFDHIIKSGRTHMQDAVPIRLGQEFTAYSRVLVKMRDK
                     IKRASEALLELNIGATAAGTGLNSDPRYQQQMVANLRDLTGFPLQPAEHLVEMTQSQY
                     AMAEVSAALRALALELIRISHDMRFMSSGPQTGIMEIVLPPVQPGSSIMPGKVNPVMF
                     EMLTMVCNQVVGNDTAVALAVSEGQLELNVMMPQMAFSTMFSVDLLK"
     gene            complement(1301..1684)
                     /locus_tag="VF276_01375"
     CDS             complement(1301..1684)
                     /locus_tag="VF276_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605539.1"
                     /translation="MAEQLVVEVGNENFGPGRARVEVSATGTVRVSNRFLGHEREVET
                     TIPPAEAARLLAQARAVRPPPPWPGRMPVPDEVSYQIAVYDGGRAIYTTECWQGDLRA
                     DPAADDLITALRGIVREAANGQIVL"
     gene            complement(1904..2668)
                     /locus_tag="VF276_01380"
     CDS             complement(1904..2668)
                     /locus_tag="VF276_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605540.1"
                     /translation="MTGPALPGRPRRATELIVHCHGERPYFAEVPYFVWGAVDYASSG
                     DAQTPTDRDWRELGLVNRETGEILEMAPVPGAGRRVVIAVRAMNPDVALRAAYFLAWR
                     TDGQAGSGPDAALETFGEMAQRLGAWDYKEATARSVRVRKDFGRAELLPFDDNGFWPS
                     WKWVGVPACARTRVGRWIMDSVLRKDTRAVYLCIEWLRRGSATPVQRDALCYALQRFT
                     GRELPSPADWVAWYAREGASVYPRPDFATWWAEVPH"
BASE COUNT          461 a          975 c         1016 g          500 t
ORIGIN      
        1 ttcttcagca ggtcgacgct gaacatggtg ctgaaggcca tctgcggcat catgacgttc
       61 agctccaact ggccctcgga cacggccagg gccacggcgg tgtcgttgcc caccacctgg
      121 ttgcagacca tggtcagcat ctcaaacatg accgggttga ccttgccggg catgatgctt
      181 gaccccggct gcaccggcgg cagcacgatc tccatgatcc cggtctgcgg cccgctgctc
      241 atgaagcgca tgtcgtggct gatgcggatc agttccagcg ccagcgcgcg cagcgccgcc
      301 gagacctcgg ccatggcata ctgcgactgg gtcatctcga ccaggtgctc ggcgggttgc
      361 agcgggaaac cggtcaggtc gcgcaggttg gcgaccatct gctgctggta gcgcggatcg
      421 ctgttcaggc cggtgcccgc cgccgtcgcg ccgatgttca gttccagcag cgcctcggat
      481 gcgcgcttga tcttgtcgcg catcttgacc agcacgcggc tgtaggcggt gaactcctgg
      541 cccaggcgga tcggcacggc gtcctgcatg tgcgtgcggc ccgacttgat gatgtggtcg
      601 aactcgcggc ccttctcggc gtaggcttcg gccatggccg tgatcgccgg gtagaaagtc
      661 tccaggttca tcagcacggc caggcgcatc gaggtcggga acacgtcgtt ggtggactgg
      721 gccatgttga cgtggtcgtt ggggtggacc ggcttgtagc tgccgcgcgg cgcgccgagg
      781 atctcgttgg cgcggttcgc cagcacctcg ttggtgttca tgttgtgcga ggtgcccgcc
      841 ccggcctgga aggggtcgat gcggaaatgc tcgcgatgcc ggcccgccag gatctcgtcg
      901 gcggcctgga cgatggcctt cgcgcgctgg tcgtccaatt ggccgagttg ctggttggtg
      961 atcgccgccg ccttcttcac ggtgacggtg gcctcgacca gcagcgggtg gggcagccag
     1021 ccggtgatgg ggaaattgtg gataccgcgc actgtctgca cgccatagag cgcgtcggcg
     1081 ggcacttcga cctcgccgag cgagtcacgt tccaggcgcg tcgttgggct gggggtgtcg
     1141 ctcatgctgt cacttgtctc ctacatatag atgggggtag gcccgaccac ggggccgggc
     1201 ggttgaccca ttctaacatt gtagcgagaa aagcgtcaag gcgcgcgccc tcattatggg
     1261 cgcgcccggt agccgccgca tgcaggctga taccctgggg ctacagaacg atctgcccgt
     1321 tcgccgcctc gcgcacaatg ccccgcaggg cggtaatgag atcgtccgcc gcgggatcgg
     1381 cgcgcaggtc gccttgccaa cactcggtgg tatagatggc cctgccgccg tcatagacgg
     1441 cgatctggta ggacacttcg tcggggacgg gcatgcggcc cggccagggc ggcggcgggc
     1501 gcacggcgcg cgcttgcgcc aggagccggg cggcctcggc aggcgggatc gtggtttcga
     1561 cctcgcgctc gtggcccaga aagcggttgc tgacgcgcac ggtgcccgtc gcgctcactt
     1621 ccacgcgggc gcgccctggc ccaaagttct cattgccgac ttccaccacc agttgctctg
     1681 ccatcaggta cctcccgcca gggacagtca ttccgcaggg tagccaggcc ggtttgaaac
     1741 cggcccctac agcgcgattg ggcggcgggc cggtttgaaa ccggccccta caacgcgatt
     1801 gagcgccggg ccggtttgaa accggcccct acaacgcgat tgaggagaac cggtctgaaa
     1861 ccggccccta ccgcggccaa gagcgccgcc cctctgcccg ccgctagtgg ggcacctcgg
     1921 cccaccaggt ggcgaagtcg gggcggggat agacggacgc gccttcgcgg gcgtaccagg
     1981 ccacccaatc ggcggggctg ggcagttcgc ggcccgtgaa gcgttgcagc gcgtagcaca
     2041 gggcgtcgcg ctgcacgggg gtcgcgctgc cccggcgcaa ccactcgatg cagaggtaga
     2101 ccgcgcgcgt gtctttccgc agcaccgagt ccatgatcca gcggcccacc cgcgtgcggg
     2161 cgcaggccgg caccccgacc cacttccaac tcggccagaa gccgttgtcg tcgaagggca
     2221 acagctcggc gcggccaaag tccttgcgca cccgcacgct gcgcgccgtg gcctccttgt
     2281 agtcccacgc gcccaggcgc tgcgccattt cgccgaacgt ctccagcgcc gcgtccggcc
     2341 cgctgccggc ttgcccgtcg gtgcgccacg cgagaaagta ggccgcgcgc agggccacat
     2401 cggggttcat ggcgcgcacg gcgatgacca cgcggcgccc cgcgccgggc accggcgcca
     2461 tctccaggat ctcgcccgtc tcgcggttga cgaggccgag ttcccgccag tcgcggtcgg
     2521 tgggggtctg cgcgtcgccg ctcgacgcgt aatcgacggc gccccagacg aaatagggca
     2581 cctcggcaaa ataggggcgt tcgccatggc agtggacgat cagttccgtc gcccggcgcg
     2641 ggcgaccggg cagcgcgggt ccggtcacag ggcctccttg cgcgtggtca agagagtagg
     2701 tcggattcgc ggctattata ctacaagcgg tgtgtggcgt ggatggcacc cgcccggcgc
     2761 gcggcacgcg tgaaaaacgc ctgataggac aactgcccgg ccaatatcgg tcttaagacg
     2821 gatgtgcatc gggcgggggg tatgatacac tggtcacgtc ctagtgtgac aacacttctg
     2881 atgaaaggat ggcgagcacg atgctaacaa acagcacccc aacgacgcaa accggtatgc
     2941 cgcggctctt tg
//
LOCUS       DASUYC010000087         3729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_160996,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000087 DASUYC010000000
VERSION     DASUYC010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160996"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1539
                     /locus_tag="VF276_01385"
     CDS             <1..1539
                     /locus_tag="VF276_01385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEX6605541.1"
                     /translation="GGYAAKNDTFNSADIALARYNPDGSLDPTFAGGGELIYDLMNGE
                     DKIYGVGLQRDGKIVAAISDGSNIIAGRFNPDGSWDAGFGGNQGVAVTGLVGHANALA
                     VQPDDRIVIAGDVRNVDGYDYAQAVTLRLDPDGALDPGFAAGGVAVTPFGDSWALSAF
                     RAVVLQPDGRIVAGGDNIPATGTEFALARYNADGSLDPTFGDGGRVTTKFTQDGPEVI
                     NALALQPDGKIIAAGRMDEFGSNFDFAICRYNPDGSRDLSIGNRGRAYTDVSTLFPDE
                     ANAVLVQPSGTIVTAGYSSDLNFTRNEWKNTALVGFLGGAAPPPPPPPPPPPPPACTV
                     PFTDIAGNPFATYIEDLYCRHIVDGYPDNTFRPYNQATRGTLAKWVVLSRGWALDTTG
                     GPHFVDVPPTDPLYRYIETAYNHGVLGGYADHTFRPNANVTRGQMSKMIVNAMGWAPV
                     TPAVPHFRDVNTANPFYSQIETIYAHGLVSGYTCGTGCLEFRWGNDNTRGQLTKVLDN
                     ALGQ"
     gene            complement(1597..>3729)
                     /locus_tag="VF276_01390"
     CDS             complement(1597..>3729)
                     /locus_tag="VF276_01390"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NB-ARC domain-containing protein"
                     /protein_id="HEX6605542.1"
                     /translation="DVAAVRALLQPAGTRLVTLIGPAGVGKTRLGLQVAAEFLDDAVD
                     GVTFVALAAVTHPDGVVPAIAQALGVRESSGAALTDDLRRYLRDRRLLLLLDNFEQVI
                     AAAPEVAALLAAAPALRVLVTSREALRLRGEHEYTIPPLALPDRRHLPPPEALSQVPA
                     VCLFLARALEVKPDFAVTDANAAAVAEICERLDGLPLAIELAAARIKTLSSAALLARL
                     SGRLALLTTGARDLPARHQTLRAALEWSYDLLDPAAQTLFARLAVFTGGGTVEAVAAI
                     CHDAPDALDGLARLVDKSLIRQDAAGDQERRFSMLETIREYAAERLEASGERAAIQAR
                     HAVYYLALAEAEEPAVRGADQLAAIARLESEHANIRAALDWFHHNPTGSDGELRLAGA
                     LWWFWQLRGYWREGRAYLERALARVSRADLRAAQARALCGAGLLAWLQGDGGKGWGYL
                     EESVAVAGACGDPSAAAHALSFLARAALDQGDFHGARRRAEESVALCRAGAGAWGLAL
                     ALRMLASAMSGLGDAARDRALLHESLAVARTVGDRWILTTMLIGLGEMARWRGADGEA
                     AGFYREALKLSRELGNKRYIAVALANLGFVTRDDPYAAEDCFLESLVVTEELGNIHLS
                     GLSLIGLAWARAAQGQPGQAAELLGTAQALLGAGGRVLDDTDRLDHERTVAAVCHTLG
                     LAAFNAAFAIGRARSLDQTLREVRHAVQ"
BASE COUNT          585 a         1399 c         1254 g          491 t
ORIGIN      
        1 ggcggctacg ccgccaagaa cgacaccttc aacagcgccg acatcgccct ggcgcgctac
       61 aatcccgacg gcagcctcga cccgaccttc gccggcggcg gcgagttgat ctacgacctg
      121 atgaacggcg aggacaaaat ctacggcgtg ggtctgcagc gcgacggcaa aatcgtcgcc
      181 gccatctccg acggcagcaa catcatcgcc ggacgcttca accccgacgg cagttgggac
      241 gccggcttcg gcggcaatca gggtgtcgca gtgaccggcc tggtcgggca cgccaacgcc
      301 ctggccgtcc agcccgacga ccgcatcgtc atcgccggcg atgtccgcaa tgtggacggc
      361 tacgactacg cgcaggcggt gaccctgcgg ctcgatcccg acggcgcgct ggaccccggc
      421 ttcgccgccg gcggcgtggc cgtgaccccc ttcggtgata gctgggcgct gagcgccttc
      481 cgcgccgtcg tcctgcaacc tgacggccgc atcgtggccg gcggcgataa catcccggcc
      541 accggtaccg agttcgccct ggcccgctat aacgccgacg gcagcctcga cccgaccttc
      601 ggcgacggcg gccgcgtcac gaccaagttc acgcaggacg gccccgaggt catcaacgcg
      661 ctggcgctgc agccggacgg caagatcatc gccgccgggc gcatggacga attcggcagc
      721 aacttcgact tcgccatctg ccgctacaac cccgacggca gccgcgacct gagcatcggc
      781 aaccgcggac gcgcctacac tgacgtttcg accctgtttc ccgacgaagc caacgccgtg
      841 ctggtgcaac cgagcggcac catcgtgacc gccggctata gctcagacct caacttcacg
      901 cgcaacgagt ggaagaacac ggccctggtc ggcttcctcg gcggcgcggc gcccccgccg
      961 cccccgcccc cgccgccgcc cccgccgccg gcgtgcacgg tcccgttcac cgacatcgcc
     1021 ggcaacccgt tcgcgaccta cattgaggac ctctactgcc gccacatcgt cgacggctac
     1081 cccgataaca cctttcgccc gtacaaccag gcgacgcggg gcacactggc caaatgggtg
     1141 gtgctgagcc ggggctgggc gctcgatacc accggcggcc cccacttcgt agacgtgccg
     1201 cccaccgacc cgctctatcg ctacatcgaa acggcctaca accacggggt gctcggcggc
     1261 tacgcggacc acacgttccg tccgaacgcc aacgtgacgc gcgggcagat gagtaagatg
     1321 atcgtcaacg ccatgggctg ggcgccggtc acccccgcgg ttccgcactt ccgcgatgtg
     1381 aacacagcca acccgttcta cagccagatc gagacgatct acgcgcacgg attggtcagc
     1441 ggctacacct gcggcacggg gtgcctggaa ttccgctggg gcaacgacaa tacccgcggc
     1501 cagttgacca aagtcctcga caacgcgctc ggccagtagc cggtcgacca gcaaaacaag
     1561 cacccgcccc tggacctgat cgggtccagg ggctttttat tgtacggcgt gccgcacctc
     1621 gcgcagggtc tggtccaggg agcgggcccg cccgatagcg aaggcggcgt taaaggccgc
     1681 cagcccgagg gtatggcaca cagcggccac ggtgcgctcg tgatcgaggc ggtcggtatc
     1741 gtcgagcacg cggccgcccg caccgagcag cgcctgcgcg gtgcccagca actcggcggc
     1801 ctggcccggc tgcccctgcg cggcgcgcgc ccaggccaac ccgatcaggc tcaagccgct
     1861 caggtggatg ttgcccaatt cctccgtcac gaccaggctt tcgaggaagc agtcctcggc
     1921 ggcgtagggg tcgtcgcgcg tgacgaatcc caggttcgcc agggccacgg cgatatagcg
     1981 cttgttgccc agttcgcgcg agagcttcag ggcctcccgg tagaagccgg ccgcctcgcc
     2041 gtccgcgccg cgccagcggg ccatttcgcc caggccgatc agcatggtcg tcaggatcca
     2101 gcggtcgccc acggtgcgcg cgacggccag gctctcatgc agcagggcgc ggtcgcgcgc
     2161 ggcatcgccc aaccccgaca tggccgaggc cagcatgcgt agcgccagcg ccagccccca
     2221 ggcgccggcc ccggcgcggc agagggccac gctttcctcg gcgcggcggc gggcgccgtg
     2281 gaaatcgccc tggtcgaggg cggcgcgggc caggaagctc aacgcgtggg ccgccgcgga
     2341 aggatccccg caggcgccgg ccacggccac gctctcctcc aggtagcccc agcctttgcc
     2401 tccgtcgcct tgcagccagg ccagcaagcc cgcgccgcag agtgcccgcg cctgggccgc
     2461 gcgcagatcg gcccggctga cgcgggccag ggcgcgttcc aggtaggcgc ggccctcgcg
     2521 ccagtagccg cgcagttgcc agaaccacca gagcgccccg gccagccgca actcgccgtc
     2581 gctgcccgtg gggttgtggt gaaaccaatc gagcgccgcg cggatgttgg cgtgctcgga
     2641 ttccaggcgc gcgatggccg ccaactggtc cgcgccgcgc acggcgggtt cctcggcctc
     2701 ggccagggcc aggtaataga ccgcgtgccg ggcctggatc gcggcgcgct cgccgctcgc
     2761 ctccaggcgc tcggcggcgt actcgcggat cgtctccagc atgctgaacc ggcgctcctg
     2821 gtccccggcg gcgtcctggc ggatgaggct tttgtcgacc agccgggcca gcccgtccag
     2881 agcatcgggg gcatcgtggc agatcgcggc caccgcctcg acggtgccgc cgcccgtgaa
     2941 gacggccagc cgcgcgaaca gggtctgtgc cgccggatcg agcagatcgt agctccactc
     3001 cagcgcggcg cgcagcgtct ggtgccgcgc cggcaggtcg cgcgccccgg tggtcagcag
     3061 ggcgagcctg ccgctcaggc gggccagcag cgccgcactg gagagggtct tgatgcgcgc
     3121 ggcggctaac tcgatggcca gcggcaggcc gtcgagccgt tcgcagatct cggccacggc
     3181 ggcggcgttc gcgtccgtga ctgcgaagtc gggcttgacc tccagcgcgc gcgcgaggaa
     3241 caggcacacc gccggcacct ggctaagcgc ctccggcggc ggcaggtggc gccgatcggg
     3301 cagcgccagc ggcggtatcg tgtattcatg ctcaccgcgc agccgtagcg cttcccggct
     3361 cgtgaccagc accctgaggg cgggcgcagc ggccagcagg gcggctactt ccggcgcggc
     3421 cgcgatcacc tgctcgaaat tgtccagcaa cagcagcagg cggcgatcac ggaggtaacg
     3481 ccggaggtcg tcggtgagcg cggcgccgct gctctcgcgc acccccagcg cctgggcgat
     3541 ggcgggcacg accccgtcag ggtgggtgac ggcagccagg gccacgaaag tcacgccgtc
     3601 caccgcgtcg tccagaaact cggcggccac ctgcaacccc aggcgggtct tgcccactcc
     3661 cgccgggccg atgagcgtca ccagccgcgt gccggcgggc tgcaagagcg cgcgcacggc
     3721 ggccacgtc
//
LOCUS       DASUYC010000088         3525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_642134,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000088 DASUYC010000000
VERSION     DASUYC010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642134"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VF276_01395"
     CDS             complement(<1..556)
                     /locus_tag="VF276_01395"
                     /inference="COORDINATES: protein motif:HMM:NF039189.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5671 domain-containing protein"
                     /protein_id="HEX6605543.1"
                     /translation="MKVARRVYIYLISYISLLLMLSGASDLLRLLLDAVLGTPAPIVD
                     ASYQREQYSRAGAMLLVGAAVWAIHWMLAQRSVAGGGPEATEERLSVLRKLLIYGVLF
                     TTAAQVFFALYGLIQGVLALHAGPGAESLSGVVSRSVPTMLVYGIGWLYYRQVSRRDN
                     AVTPERGGAATVRRWYFYLVNFGAL"
     gene            complement(591..1091)
                     /locus_tag="VF276_01400"
     CDS             complement(591..1091)
                     /locus_tag="VF276_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605544.1"
                     /translation="MDTASTPSAAPPQTDRFLIGILVGVVVLLAAAGLSLLVLRRPPP
                     QLPADSPGGTIQRFYSALEQRNYNQAYSLLSDNMEHKPTRDSFTRAMADQYTYGTQAA
                     RASIDSEQIDGATATVTVRVTYYYGGGPPLLGSNEYTSTETFTLAQEAGAWRITALPP
                     RYQAPS"
     gene            complement(1377..2342)
                     /locus_tag="VF276_01405"
     CDS             complement(1377..2342)
                     /locus_tag="VF276_01405"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HEX6605545.1"
                     /translation="MTDAPFAWDRLPRGFLALSPMAGVTDSPFRQICKRMGADVLFTE
                     MVSAEAFLHHHNKTTAIMLHYEPFEHPIICQLMGSDPVRLAEAARRIEDYGFDGVDLN
                     MGCPAQKIYGNRCGSSLLEYPDEAAEIVYAMSRATRLPVSVKMRAGVTGNVQPAFAQR
                     MIEAGAKALTIHPRTKEQAYRGQSDWAITRAIAAASPLPVMGSGDLMRWADVERLFAT
                     TGVQGAFIARGSMGNPWIFRRADSAPSIEELVAVVRRQAQLSLGLKGPHGLIELRKHL
                     GWYCKGFAGASDIRRRATSVETMDDVEALLAAVLDAAGAGTPIAA"
     gene            2482..2769
                     /locus_tag="VF276_01410"
     CDS             2482..2769
                     /locus_tag="VF276_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605546.1"
                     /translation="MANKQTDPVPQSPEVQPADVREELDQTQVSHEGRPEATRGGKPS
                     THMGATEDAVTPVVPPMRGPGNLVGPSAPGDSHDPPTKDQLIDPVDEITPG"
     gene            2896..>3525
                     /locus_tag="VF276_01415"
     CDS             2896..>3525
                     /locus_tag="VF276_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEX6605547.1"
                     /translation="MVSTPDVQTRPQPASAPERPEVVNDFSIVAATVNGSGSQTANST
                     LIRALFKMGIPVNGKNLFPSNISGLPTWYTIRVSKDGYIARREVTEVLVAFNPQTADA
                     DLASLPVGGVCIYPEDLTFANRRADVTYYPLPVKEMVKNSGADAKLRDYVANMVYVGA
                     LAELLHIEFDEIKNALSYHFKGKTKAVDMNYGVVSAAAEYTRANLPKQDP"
BASE COUNT          640 a         1097 c         1213 g          575 t
ORIGIN      
        1 acagggcacc gaagttgacc aggtagaagt accagcggcg caccgtggcc gcgccgcctc
       61 gttccggcgt gacggcgtta tcgcgccggc tcacctggcg gtaatagagc caaccgatcc
      121 cgtagacaag catggtgggc acgctgcgac tcaccacgcc ggataggctc tcggcgccgg
      181 ggccggcgtg caaggccaga acgccctgga tcagcccgta cagggcgaag aagacctgcg
      241 cggcggtggt gaagagtacg ccgtagatca agagcttgcg caagacggac aggcgctcct
      301 cggtcgcttc cgggccgcca cccgccacgc tgcgctgcgc cagcatccag tggatggccc
      361 agaccgcggc gccgaccagc agcatcgccc cggcgcggct gtactgctcg cgctggtagc
      421 tcgcgtcgac gattggcgcc ggcgtgccca ggacggcgtc gaggagcagg cgcaagagat
      481 cggatgcgcc tgacagcatc agcaggaggc tgatgtagct gatcaggtaa atatagaccc
      541 ggcgtgcgac tttcatggcc ggcctcctct gttagcgcct ggcttatctc tcaggatggt
      601 gcctggtacc ggggcggaag ggcggtgatg cgccaggcgc cggcttcctg ggcgagcgtg
      661 aaggtttccg tcgaggtata ttcgttggag cccaatagcg gcgggccgcc gccgtaatag
      721 taggtcacgc gcaccgtcac cgtggccgtc gcgccgtcga tctgctcgga gtcgatactg
      781 gcgcgcgccg cttgcgtacc gtaggtgtac tggtcggcca tggcgcgcgt gaaggagtcg
      841 cgcgtgggct tgtgctccat gttgtcgctt aaaaggctgt aggcctggtt gtagttccgc
      901 tgttccaggg cgctgtaaaa gcgctggatc gtgccgcccg gcgaatcggc cggtaactgg
      961 ggcggcggcc ggcgcaagac cagcagactt agcccggcgg cggccagtaa cacaacgacc
     1021 ccgaccagga tgccgatcag gaagcggtcg gtttgcggcg gcgctgcgga tggcgtggac
     1081 gctgtatcca tcggtatcac ctctccacac gctggtagcc ggcagcggca acttgccgca
     1141 tagctgcccc acggggcgcc gggttgagtc ctgctgctgc caacggcgct gctcgccgat
     1201 ggcagggcgg gtcccaccac tatagttgta gagcgcgaag cggaaagacc gggttaacgg
     1261 cgggcgacaa cgtggtactg gatggatcgg gtcaggatcg gagcgggacg agttaacata
     1321 actgctaaca gcaatgcgat tagcctgtta tcgggcggct gggccggcga gcggccttat
     1381 gcagcgatgg gcgtgcccgc gcccgcggcg tcgaggacgg cggccagcag ggcttccacg
     1441 tcgtccatag tctcgacgct ggtggcgcgg cggcggatgt cggaggcgcc cgcgaagccc
     1501 ttgcagtacc agcccaggtg cttgcgcagt tcgatcaggc cgtgcggccc cttgagcccc
     1561 aggctcagtt gcgcctgccg gcggaccacg gccacgagtt cctcgatgga cggggccgag
     1621 tcggcgcggc ggaagatcca ggggttgccc atggagccgc gggcgatgaa ggcaccctgc
     1681 accccggtcg tggcgaacag ccgctccacg tcggcccagc gcatcaggtc gccgctgccc
     1741 atcacgggca ggggggacgc cgccgcgatg gcgcgggtga tggcccagtc ggactggccg
     1801 cggtatgcct gctccttggt tcgcgggtgg atcgtcagcg ccttggcccc ggcctcgatc
     1861 atgcgctggg caaaggcggg ctgcacgttg ccggtcacgc ccgcgcgcat tttcaccgac
     1921 accggcaggc gcgtggccct gctcatggca tagacgatct ccgccgcctc gtcgggatac
     1981 tccagcagcg aggagccgca gcggttgccg tagatcttct gcgccgggca gcccatgttg
     2041 aggtccacgc cgtcgaaccc gtagtcctcg atgcgccgtg ccgcctcggc cagccgcacc
     2101 gggtcgctgc ccatcaactg gcagatgatc gggtgctcaa acggctcgta gtggagcatg
     2161 atcgccgtgg tcttgttgtg gtggtgcagg aacgcctcgg cgctgaccat ctcggtgaac
     2221 agcacgtcgg cccccatccg cttgcagatc tggcggaagg ggctatcggt gacgccggcc
     2281 atgggcgaga gggccaggaa cccgcgcggc aggcggtccc aggcgaaagg tgcatcggtc
     2341 atagcccaat tgtaccagaa aacgcgggcc tatctcaacc gggctggcgc gcggcagata
     2401 aagccgagat cgtttacaat agggtaaggc tgcgcacagg tgcgcggcct ggtatacgag
     2461 acgatgcaag gaggaacgac gatggcaaac aagcagaccg accccgtgcc gcagtcccca
     2521 gaggtgcaac cggcggatgt gcgcgaagag cttgatcaga cgcaggtgag ccacgaaggc
     2581 cgcccggaag cgacccgcgg gggcaagcca tccacccaca tgggcgccac cgaagacgcg
     2641 gtcaccccgg tggtgccgcc gatgcgcgga ccgggcaacc tggtcggtcc gtcggccccc
     2701 ggcgacagcc acgacccgcc caccaaggac caactgatcg acccggtcga cgagatcacc
     2761 cccggctgag cccgccgccc cgatgctgag gatgcgcgga aagccctttg ggtgtataat
     2821 aatggatgca agtaatgtaa ggtgtgccca gtgggccgca cgactccctg ggttttctac
     2881 gaggaggtag actgcatggt aagcacgcca gacgtgcaaa ctagacccca accggcatcc
     2941 gcgccggagc ggcccgaggt cgtcaacgac ttctcgatcg tcgccgccac ggtgaacggg
     3001 tcggggagcc agacggcgaa ttcgacgttg atccgcgcgc tcttcaaaat gggcatcccg
     3061 gttaacggta agaatctgtt cccgtcgaat atctcgggcc tgccgacctg gtacaccatc
     3121 cgggtcagca aggatgggta tattgcacgc cgtgaagtga ctgaggtgtt ggtggctttt
     3181 aacccgcaga cggccgatgc cgacctggcg tctctgccgg tgggcggcgt ctgcatctat
     3241 cctgaggatc tgacctttgc caaccggcgc gcggatgtga cttattatcc gctcccggtg
     3301 aaggagatgg tcaagaattc gggcgcggac gccaagttgc gcgactatgt ggcgaacatg
     3361 gtctatgtcg gcgcgctggc ggaactgctg catattgagt ttgacgaaat caagaacgcc
     3421 ctgtcgtacc acttcaaggg taaaaccaaa gcggtagata tgaactatgg cgtggtcagc
     3481 gcggcggcag aatatacccg ggccaacctg cccaagcaag atccg
//
LOCUS       DASUYC010000089         4261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_767337,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000089 DASUYC010000000
VERSION     DASUYC010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767337"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1090
                     /locus_tag="VF276_01420"
     CDS             <1..1090
                     /locus_tag="VF276_01420"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX6605548.1"
                     /translation="DRPYLVLPPIFKDWAATQPKTLFPPTATCCFPTPVPTEPPTPDI
                     QETPVPGLVPPIQIPNPVPRITPARDDDLPGGVVNITAPRPGAGVGGQVTITGNAVAK
                     DFDYYTVEYGAGAAPASWVAINGQTTMSVRDGVLAIWNTNGLPEGPYTLRLTVANKQQ
                     QRRQYLVQVSIERTPLKVQLTAPVAGQPYLSGNPVALAAAVTGPKDLAGVEFYVDNVR
                     IAVDYDAPYQATWTATGGDHTLDAVAYSATGRRATSDAVQITVADPPTPTATPRPSFT
                     ILYPQDQASVNASTMNVLVAAGPGSGITRVDFYVDGWNVSTAQGQDTFQIQWQAIPGR
                     HTIMAIGYNAAGTEVARAQATVYTNPGP"
     gene            1379..2719
                     /locus_tag="VF276_01425"
     CDS             1379..2719
                     /locus_tag="VF276_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006348422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605549.1"
                     /translation="MEQQTQVVTNADELQDAVKRGVAHITVEGEIRGMPMLALQPGQT
                     LTGGTLYFGASGVQLSRDNTLSDITIRTSETERAILNDTGVTDLGTLTLHNVRTTGQV
                     ALLLGGATRGGHVRVENLHIERADVRGRAVRPHAYGVDAMQGAFTLWNTHTQNVVITA
                     ELLNLSAGSVESPVRGSGVFVGGQGQPDGTPEGGRVQVKLLTTGEIHADGGIAENTPD
                     LISGGVFVIYGAFVEHVENLGAVTTYGPNDMVLDNWGNVREWIARAPVVSRGTSGIGF
                     VQFGEIGSVRVEAPIETFGKGARGFNLYAGNLQDAAFHSINTHADGAVGIQVSRDLPR
                     LSVEGDVTTQGGESDSLVKGKVIKLKAYAVSVQPGGQIGELSVGGELSTEGQEVVTLQ
                     VQGKIDRLDVRGGIHALGEHSDAVMIEGGDVPLEGTKITARDGERIRVSQPKHA"
     gene            2904..>4261
                     /gene="htpG"
                     /locus_tag="VF276_01430"
     CDS             2904..>4261
                     /gene="htpG"
                     /locus_tag="VF276_01430"
                     /inference="COORDINATES: protein motif:HMM:NF003555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HEX6605550.1"
                     /translation="MADAEATASPQPAPESLEFRAEIRQLLNILAHSLYTEREIFLRE
                     LISNASDALNRLRFEQLTNQDILDPDAELAIRITVDQDARIVRVSDTGIGMTREELIE
                     NLGTIAHSGAMSFLTRLEQDPKHGADIIGQFGVGFYSVFMVADEVRVSSRSYQPDAEA
                     VEWVTRGENTYTVGPANKATRGTTVELTLREDAAEFANTWRLESIIRKHSDFIAFPIY
                     VGDGDKPVNRQTAPWRRAPREVTEEQYTEFYKQLTFDPEPPLSHIHLVTDAPVQVRAL
                     VYIPRHREQGMLRLRTEEGLQLYSHNVLIQEYSTDLLPKHFRFVTGVVDSEDLPLNIS
                     RETVQMQSNRVMERIRNLLTKRVQGELERLAADAPDDYHTFWTAFSVYLKEGVATDPG
                     AREPLARLLRYPSSATRGDELTSLQAYVDRMKPEQDAIYYVLADDQRAAAASPHLDPF
                     KAR"
BASE COUNT          837 a         1401 c         1353 g          670 t
ORIGIN      
        1 cgaccggccc tacctggtgc tgccgccgat ctttaaggac tgggcagcaa cccagcccaa
       61 gacgctcttc ccgccgacgg ccacctgctg cttcccgacg ccggtgccca ccgagccgcc
      121 cacgcccgac atccaggaga cgccggtgcc cggcctggtg ccgcccatcc agatccccaa
      181 cccggtgccg cgcatcaccc cggcgcgcga cgacgatttg cccggcggcg tggttaacat
      241 caccgcgccg cgccccggcg ccggggtggg cgggcaggtc accatcaccg gcaacgccgt
      301 ggcgaaggac ttcgactact acacggtgga gtacggcgcg ggcgcggccc cggcgagttg
      361 ggtggcgatt aacggccaga ccacgatgag cgtgcgcgac ggcgtgctgg cgatctggaa
      421 caccaacggc ctgccggaag gcccctatac gctgcgcctg accgtggcga acaagcaaca
      481 gcagcgccgc cagtacctgg tccaggtcag tatcgagcgc acgccgctca aggtgcaact
      541 caccgcgccc gtcgccgggc agccctacct cagcggcaat ccggtggcgc tggccgccgc
      601 cgtcaccggg ccgaaggacc tggcgggcgt cgagttctac gtggacaacg tgcgcatcgc
      661 cgtggactac gacgcgccct accaggccac ctggaccgcg acgggcgggg accacactct
      721 cgacgccgtg gcctacagcg cgaccgggcg gcgggccacc agcgacgcgg tgcagatcac
      781 cgtcgccgac ccgcccacgc ccacggccac gccgcgcccg tcgttcacca tcctctaccc
      841 gcaggaccag gccagcgtca acgcctcgac catgaacgtg ctcgtggccg ccgggccggg
      901 gagcggcatc actcgcgtcg atttctacgt ggacggctgg aacgtgagca cggcccaggg
      961 ccaggatacg ttccagatcc agtggcaggc cattccgggc cgccacacca tcatggcgat
     1021 cggctacaat gccgcgggca cggaggtcgc ccgcgcccag gccaccgtct acaccaatcc
     1081 cggcccgtag cggcagccgg agcgcgccgt atttgcaacc ggcgcccgca agcggtagaa
     1141 tcaacgtggg agcccggttt tgaaccggcg ccgacaacca ttagcatcaa cgtaggggcc
     1201 ggttttgaac cggctcctat ccatgttagc accgtgcccg ttttgagtcg cgctcgaaat
     1261 ggtcgcacga gatgaggagg atcttggtct cgttcttgct gggaactgct gatcaggaac
     1321 gccgcggccg gagtggcgct ggaaacagcg ctcctgtaca ccagctcagg gggagtatat
     1381 ggagcaacaa acccaagttg tgacgaacgc agatgagctt caggacgcag tgaagcgcgg
     1441 cgttgcgcac attaccgtcg aaggtgagat tcgcggaatg ccgatgcttg cccttcaacc
     1501 cggacagact ttgaccggag ggactttgta tttcggcgcg agcggcgtcc agttgagccg
     1561 cgacaacacg ctctccgaca tcaccattcg cacaagcgaa acagagcggg ctattttaaa
     1621 cgatacgggt gtcacggatt taggcacact cacgcttcac aacgttcgca cgacgggaca
     1681 agtggcgttg ttgctcggcg gcgcgacgcg cggcgggcat gtgcgcgtcg agaatcttca
     1741 tatcgagcgc gccgatgtgc gcgggcgcgc cgtgcgacct cacgcctacg gtgtggacgc
     1801 gatgcagggt gcgttcacct tgtggaatac gcacacccag aatgtggtga tcactgccga
     1861 actgctcaac ctttcggcag gcagtgtaga atcgcccgtg cgcggcagcg gcgttttcgt
     1921 cggcgggcag ggccagccgg acggcacacc ggaaggcggg cgggtgcagg tgaagcttct
     1981 taccaccggc gagatccacg ccgacggcgg catcgccgag aatacgcctg atctgattag
     2041 cggcggcgtg ttcgtgattt acggcgcgtt tgtggagcac gtcgagaacc tcggagcggt
     2101 tacgacttac ggcccgaatg atatggtgct tgataactgg ggcaacgtgc gcgagtggat
     2161 cgcccgtgcc cctgtcgttt cacgcggaac gagcggcatc ggatttgtgc aatttgggga
     2221 aatcggcagt gtgcgagtcg aggcgcctat cgagaccttc ggcaagggcg cgcgcggctt
     2281 caatctctac gccggaaatt tgcaggatgc tgcattccac agcataaaca cacacgctga
     2341 tggggcggta ggcattcagg ttagccgcga tttgccccgc ttgagcgtcg agggcgatgt
     2401 gaccacgcaa ggcggcgaaa gtgatagttt ggtaaaaggt aaagtgatca agctcaaggc
     2461 atatgcagtg agcgtgcagc caggcggtca gatcggcgag ttaagcgtcg gcggcgaact
     2521 gagtaccgaa gggcaagaag ttgtaacgct ccaagtgcag gggaaaattg atcgtttgga
     2581 cgtgcgagga ggcattcatg cactggggga gcattcggat gctgttatga tcgaaggcgg
     2641 cgatgtcccg cttgaaggta caaagattac tgctcgtgat ggcgagcgca tccgcgtatc
     2701 ccagccaaag catgcgtaaa tcgacatgga acccaccagc ttcgaccgca tgaaacctat
     2761 ccaatggcac ggaacacggg gcgcgaactc tccttgtcgt cggtacggcg ggcgcggtga
     2821 ctccggcttt gcatcattca tcgaaaccga gaaaccctga tgccaaagca attagcacca
     2881 atgaaataag gagggagacc cctatggcag acgccgaagc gacggcgagt ccgcaaccgg
     2941 cgcccgaatc gctggagttc cgcgccgaga tccggcagtt gttgaatatc ctcgcccact
     3001 cgctctatac cgagcgcgaa atcttcctgc gcgagttgat ctccaacgcc tcggacgccc
     3061 tgaaccggct gcgctttgaa caactgacca accaggacat cctcgacccc gacgccgaac
     3121 tggcgatccg catcaccgtc gaccaggacg cgcgcatcgt gcgcgtcagc gacacgggca
     3181 tcggcatgac gcgcgaggag ttgatcgaaa acctgggcac catcgcccac tccggcgcga
     3241 tgagcttcct gacccggctg gagcaagacc ccaagcacgg cgccgacatc atcggccagt
     3301 tcggggtggg cttctactcg gtgttcatgg tggccgacga ggtgcgcgtc agctcgcgct
     3361 cgtaccagcc cgacgccgaa gcggtcgagt gggtgacgcg cggcgagaac acctataccg
     3421 tcggcccggc caacaaggcc acccgcggca ccacggtcga gttgacgctg cgtgaggacg
     3481 ccgccgagtt cgccaacacc tggcggctgg aaagcatcat ccgcaagcac tccgacttca
     3541 tcgccttccc gatctacgtg ggcgacggcg acaagccggt gaaccgccag acggcgccct
     3601 ggcggcgcgc gcccagagag gtcacggagg agcagtacac cgagttctac aagcagctca
     3661 ccttcgaccc cgagccgccg ctgagccaca tccacctcgt caccgacgcc ccggtgcagg
     3721 tgcgcgccct ggtctacatc ccgcgccacc gcgagcaggg catgttgcgc ctgcgcaccg
     3781 aggaagggct gcaactctac tcgcacaacg tgctgatcca ggagtatagc accgacctgc
     3841 tgcccaagca cttccgcttc gtgaccggcg tggtcgactc cgaggacttg ccgctgaaca
     3901 tctcgcgcga gaccgtgcag atgcagagca accgcgtgat ggagcgcatc cgcaacctgc
     3961 tgaccaagcg ggtgcagggc gaactggagc gcctggccgc cgacgcgccc gacgattacc
     4021 acaccttctg gacggcgttc agcgtctatc tcaaagaggg tgtggcgacc gaccccggcg
     4081 cccgcgagcc gctggcccgc ctgctgcgct acccgtcctc cgccacccgc ggcgacgagt
     4141 tgacctcgct gcaagcctac gtggaccgca tgaagccgga gcaggacgcc atctactacg
     4201 tcctggccga cgaccagcgc gcggcggcgg ccagcccgca cctggatccg ttcaaggcgc
     4261 g
//
LOCUS       DASUYC010000090         7102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_388942,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000090 DASUYC010000000
VERSION     DASUYC010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 7102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7102
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388942"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..1663
                     /locus_tag="VF276_01435"
     CDS             122..1663
                     /locus_tag="VF276_01435"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEX6605551.1"
                     /translation="MLRLENLSHSRKMGMIGGHIFLIAVLVLLGISTDPAPVHPLRAA
                     PVLLAQAMAAPQAMLHVIVQKQPAAADVEAQVARLGGRVTRDLHIIHAFGADVPGRAM
                     AQLAATSGVRWISADTPVVAAGCNAPCINTGNLKSSYPQTVRAKNVWNTSPGFRQGQG
                     IGVAVLDSGITPHADLGNRVVANVTVGASTTVSDTYGHGTHVAGIIGGDGTASNGTYI
                     GIAPKVNLVNVKISDDTGAASAADVVAGLQWVNDNKSTYNIRVVNLSLNSSVAESYNV
                     DPIDAAVEILWFNKVVVVVSAGNDGKNALNPPANDPFVITVGAIDDKGTGGTSDDRMP
                     AFSGYGTTSAGFAKPDLVAPGVNLISDLAGSQATLPTQHPDHLTSGFTGASAYFRMSG
                     TSMAAPVVAGAVALLLQDEPNLTPDQVKYRLISTTHALAGAAGPGGGAGLLNIEAALK
                     GSSTQNANTGIPASQMLWTGNEPVTWDSINWNSINWNSINWNSVNWNSINWNSINWNS
                     DDWGP"
     gene            1782..4655
                     /locus_tag="VF276_01440"
     CDS             1782..4655
                     /locus_tag="VF276_01440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX6605552.1"
                     /translation="MSRRAWAYIWGVLAAGTLLGGWLLPGLADEAAWPTCIALTLLAT
                     LALLFKTVFEIRHQTDGGSTAYSPVLVFFFAGVLLLPPAFLVPLVVIPHLIDWGKERW
                     NKTGNLPAWYIQPFNIATYLIVGVFACALYRGLNIGPVTGLSPAPVLGVMGIILAYVV
                     LNSLFIGLALVLARGLSWQATGVLNPENLLPDFIMLSLGYAVALLWNLNPWLVLPVLS
                     PLVLVSRALQVPQLKKEAQTDGKTGLANARHFGNLAKAELDRAARFNRPLALVMADLD
                     LLRDVNNTYGHLAGDVVLTGIGQIIRETVREYDVAGRFGGEEFALVLPEAGPAEAQRL
                     AERLRQAVEAASFKVTTSPTPIHVTMSLGVACFPTDGDTLTGLIHEADVAVYQAKLKG
                     RNRVVCAADVPHHIRLGDVTAGPGGEESARAYLAAHAPSLPAAPRPPAPPAALDAAAP
                     AANAANASDPAAGSPAVDAAGGPAEAGAESHDAGGPSGLLWMFVGGVTAIALAVTLLT
                     GAAGEPPPLVPLLLFALLAGLFEWLQINVYSDNTTSVSVAITFAAALVTGVPGVAVVS
                     AVIALVHYLRRRPQPYKTAFNWATHLLAGAAVVLIMQSVPLPLQLENVLWLVAPTVLA
                     ALVQYAIESGLIAGAISLAGGVRFIGTWRKQLQWLAAHYVVLGLMGLFLSTAYTSLGT
                     LGVAVFTLPVLMMRYAQQQYVDRTKDSVQELTRMNGELARANREIVSASRSIQQLNDE
                     LFLTLSKILDVRDPYVGGHASQVSRYATAIAVELGLPRERVEQVRQAGFLHDIGKIAI
                     SEQILHKPSKLTAEEYEYVKGHAAIGAEFLATSQALRHLAPMVRGHHERWDGAGYPDG
                     LRGEEIPLEARILAVCDAVEAMASDRPYQPAKSLAEVIAEVRRCAGTHFDPAVVEVFI
                     RVAQRDGATLVVNSAREVLRKQADSSDPFSLETQVFMPQPAPA"
     gene            4817..6403
                     /locus_tag="VF276_01445"
     CDS             4817..6403
                     /locus_tag="VF276_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605553.1"
                     /translation="MKSPTTESPATSPLRRPRLGRLRRGGCLLRVLLVLLVLGGGGAL
                     TLKLFGNDILPPLVDTVRGVVGPEAIAEIENRYYGFVETTHQWQRDLGLRPKVVAPWA
                     VSTAPPRPTDPVTVADAGDSQDIVPAVPSPTPDLTVIHELPGELPSETPTPVPETRVI
                     DLLPATVAPPTATPPPTATRPPKRATRTPRPVKHTATPVPKLPAAQPTEPPTAVAVEP
                     AAPAVSPSPVIEAAPPPVAPPPVYTGAGPAPMAPIIQDGALPGEGVWTADGFPAAPDG
                     GPPAFWKTYLRPDAARPDAMVYMVRVDLRRVRLHMVAGTKEPVSSLGTHGSGVVPPAD
                     QALLAGAFNGGWKSVHGNYGMRVNGLQIVPPNPRPDTATLALHADGRMELAGWKTLQN
                     AGDLVSYRQNCPLLIDNGTINVSTHITSTWGLSLLNEMYVWRSGVATTPDGALIYAAG
                     TPISADELAAAFQHAGATMAMQLDINSAWVQWLSYDRNSAGRPQATPLVPEMAYRRDQ
                     YLSPNDRDFFYLTWRMSTAR"
     gene            complement(6687..6944)
                     /locus_tag="VF276_01450"
     CDS             complement(6687..6944)
                     /locus_tag="VF276_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605554.1"
                     /translation="MSNNYRFFHIEVNERALTDELYDYVTDPSPARNDRPVIEELDEV
                     APDMVPVLECMVLDGVEEREHVADYVHSVVHGEGQWRLETR"
BASE COUNT         1135 a         2391 c         2407 g         1169 t
ORIGIN      
        1 cggtaagctg gaactcggta agctggaact cggtgagttg gaatagcgat tattggggcc
       61 cgtgaccgca cgcatgatgg gcagcgcacc gggagccgct acgctgccca aggagttccc
      121 gatgttgaga ttagagaacc tgtcgcattc tcggaagatg ggcatgattg gggggcatat
      181 cttcctcata gcggtcctgg tgctgcttgg gataagcact gaccccgcac ccgtgcatcc
      241 gttgcgagcg gcgcccgtct tgctggccca ggcgatggcc gccccgcaag ccatgctgca
      301 cgtgatcgtc cagaaacagc cggctgcagc cgatgtggaa gcgcaggtgg cgcgcctggg
      361 tgggcgggtc acgcgcgacc tgcacatcat ccatgccttt ggggccgacg tgccggggcg
      421 ggcaatggcc caactggcgg cgacgtccgg ggtgcgctgg atttccgcgg atacccccgt
      481 cgttgctgcc gggtgcaatg cgccctgtat caataccggg aatctcaaga gcagctatcc
      541 gcaaacggtt cgcgcgaaga acgtgtggaa tacgtctcct ggtttccggc aaggccaggg
      601 gatcggcgtg gcggtgctgg acagcgggat caccccgcat gccgacctgg gcaaccgggt
      661 ggtggcgaat gtcaccgtcg gcgcctcgac gaccgtgagt gatacctacg ggcatggcac
      721 gcatgtcgcc ggaattatcg gcggcgacgg cacggcctcc aacggcacct atatcggtat
      781 cgcgcccaag gtcaacctgg tgaacgtcaa gatcagcgac gataccggcg ccgccagcgc
      841 cgccgatgtc gtggccgggc tgcaatgggt gaacgacaac aaaagcacat acaatattcg
      901 cgtcgtcaac ctctcgctca acagtagcgt ggccgagtcc tacaacgtgg acccgattga
      961 tgccgcggtg gaaatcctgt ggttcaacaa ggtagtcgtc gtggtgtcgg caggcaacga
     1021 tgggaaaaat gccctcaacc cgccggccaa cgatccgttc gtgatcacgg tcggggcgat
     1081 cgacgacaag ggcacgggcg gcacctccga cgatcgcatg cccgccttct cgggctacgg
     1141 cacgacgagc gccgggttcg ccaaacccga cctggttgcg ccgggggtca atctcatcag
     1201 cgacctggcc ggatcgcagg ccaccttgcc cacgcaacac cccgaccact taaccagcgg
     1261 ctttaccggg gcctcggcgt acttccgcat gtcgggcacc tcgatggccg cgcccgtggt
     1321 ggccggggcc gtcgccttgc tgctgcagga tgagcccaat cttacgccgg atcaggtaaa
     1381 ataccggctg atctcgacga ctcacgcgct ggccggggcg gccggccctg gcgggggcgc
     1441 gggcctgctc aatatcgagg ccgccctgaa gggcagcagc acccagaacg ccaacaccgg
     1501 tatccccgcc agccagatgc tctggaccgg gaacgaaccg gtgacctggg actcgatcaa
     1561 ctggaactcg atcaactgga actccattaa ctggaattcc gtgaactgga actcgatcaa
     1621 ctggaactcg atcaactgga acagcgacga ctggggtccc tgaccaggac cgtagcggcg
     1681 tgtgagtggt ttgcgaatat acctgggcag gccgggcgcg ggtctgccca gccgggggcg
     1741 aggagtggtg acttctcgct ccgcttggtt ggagaaaagc tatgtcgagg cgagcctggg
     1801 cctatatctg gggagtactg gcggcgggga ccctgctcgg cggctggttg ctgccgggcc
     1861 tggccgacga ggccgcgtgg ccgacctgca tcgccttgac gttgctggca accctggcgc
     1921 tgcttttcaa gactgtcttc gagatccggc accagaccga cggcgggtcc accgcctata
     1981 gccccgtgct cgtctttttc ttcgccgggg tgctgttgtt gccgccggcg ttcctggtgc
     2041 cgcttgtcgt catcccgcat ctgatcgact ggggcaaaga gcgctggaac aagaccggta
     2101 atttgcccgc gtggtatatt cagcctttca acatcgccac ctatctcatc gtgggggtgt
     2161 ttgcctgcgc gctgtaccgg gggctaaata tcgggccggt aacgggcctg tcgcccgcgc
     2221 cggtgctggg cgtcatgggc atcatcctgg cctatgtcgt cctgaacagc ctgtttatcg
     2281 gcctggcgct ggtgctggcg cgcgggctgt catggcaggc caccggggtg ctcaacccgg
     2341 agaacctgct gcccgacttt atcatgctct cgctgggcta cgccgtggcc ttgctgtgga
     2401 acttgaatcc gtggctggtg ctgccggtgc tctccccgct ggtgctggtg tcgcgggcgc
     2461 tgcaggtgcc ccaactgaag aaagaggcgc agaccgacgg caagactggg ctggcgaacg
     2521 cgcggcactt cggcaatctc gccaaagccg agctcgatcg tgcggcgcgc ttcaaccgac
     2581 ccctggcgct ggtcatggcc gacctcgact tgctgcgcga tgtcaacaac acctacggcc
     2641 acctggccgg cgatgtggtg ctgaccggga tcggccagat tattcgcgag acggtgcgcg
     2701 aatacgatgt ggccgggcgg ttcggcggcg aggaatttgc cctggtgctg cccgaagcgg
     2761 ggccggcgga agcccagcgg ctggccgagc gcctgcgcca ggccgtcgag gcggccagct
     2821 ttaaagtcac caccagcccc acgccgatcc acgtgacgat gagcctgggc gtggcctgct
     2881 tcccgaccga tggcgatacg ctgaccgggc taatccacga ggccgatgta gccgtctacc
     2941 aggccaagct gaaagggcgc aaccgcgtgg tctgtgccgc cgatgtgccc catcacatcc
     3001 ggctcggcga tgtgacggcg gggccggggg gcgaagagtc ggcccgcgcc tacctggccg
     3061 ctcatgcgcc cagtctgccg gcagccccgc ggcccccggc cccgccggcc gcgctagacg
     3121 ccgccgcgcc cgcggcgaat gccgccaatg cgagcgaccc cgccgctgga tcgcccgccg
     3181 tggacgccgc gggcggccct gccgaagcgg gcgcggagtc gcacgacgcg ggcgggccgt
     3241 caggcttgct ctggatgttc gtcggcggcg tcacggctat cgcgctggcc gtcaccctgc
     3301 tgacaggggc cgccggcgag ccgccgcccc tcgtgcctct gctgctgttt gcgctactgg
     3361 ccgggctgtt tgaatggctg cagatcaacg tctatagcga caacaccacc tcggtctcgg
     3421 tggcgatcac ctttgccgcc gccctcgtca ccggggtgcc gggggtggcc gtggtcagcg
     3481 cggtgatcgc gctggtccat tacctgcgcc ggcgcccgca gccgtacaag accgccttca
     3541 actgggccac tcatctgctg gccggggcgg cggtggtgct gatcatgcag tcggtgccgc
     3601 tgccgttgca actggagaac gtgctctggc tggtcgcgcc gacggtgctg gccgccctgg
     3661 tgcagtacgc catcgagagc gggctgatcg cgggggcgat cagcctggcg ggcggcgtac
     3721 gctttatcgg cacctggcgc aagcaactgc aatggctcgc cgcgcactat gtcgtgctcg
     3781 gcctgatggg gctcttcctg agcacggcct acacctcgct tggcaccctg ggcgtggccg
     3841 tgttcaccct gccggtgctg atgatgcgct atgcccagca gcaatacgtg gaccgcacca
     3901 aggacagcgt ccaggaactg acccgcatga acggcgagct ggcccgcgcc aatcgcgaga
     3961 tcgtcagcgc cagccggtcg atccagcaac tcaacgacga gttattcctg accctgtcca
     4021 agatcctcga cgtgcgcgat ccgtatgtcg gcggccatgc ctcgcaggtg tcgcgctatg
     4081 ccacggccat cgccgtcgaa ctcggcttgc cgcgcgaacg ggtcgagcag gtgcgccagg
     4141 cgggcttttt gcacgacatc ggcaagatcg ccatttccga gcagatcctg cacaagccga
     4201 gcaagctcac cgccgaggaa tacgagtacg tcaagggtca cgcggcgatt ggcgctgagt
     4261 ttctggcgac cagccaggcg ctgcggcatc tggcgccgat ggtgcgcggc catcacgagc
     4321 gctgggatgg cgcgggctac cccgacggct tgcgcggcga ggagatcccg ctcgaagcgc
     4381 gcatcctggc cgtctgcgac gccgtcgagg cgatggcctc agaccggccc taccagccgg
     4441 ccaagtcgct ggccgaggtg atcgccgagg tgcgccggtg cgccggcacg catttcgacc
     4501 cggcggtggt cgaggtcttc atccgggtgg cccagcgcga cggcgctacc ctggtcgtga
     4561 actcggcccg cgaggtgcta cgcaagcaag ccgatagttc cgacccgttc tcgctggaaa
     4621 cgcaggtgtt tatgccccag cccgccccgg cctgacctgc gggggatcaa acggggcaaa
     4681 attgtgacgc cgttgtaacg tccctaggtt tgacgagggc tgcgctttcg ggtaacatta
     4741 ccgtgtaaca taccgccccc gacgaccgga atgacgcacg gtcgttgact agcgctcaat
     4801 aaaggccccg tcacgtttga aatcaccgac cactgaatca cccgccacct cgccgctgcg
     4861 ccggccccgg ctgggccgcc tgcggcgcgg gggctgcctg ctgcgcgtcc tgctcgtcct
     4921 gctcgtgctg ggtgggggcg gcgcgctgac gctgaaactg ttcggtaacg acatcctgcc
     4981 gccgctggtc gatacggtgc gcggcgtcgt cgggccggag gcgatcgccg aaatcgagaa
     5041 ccgctactac ggcttcgtcg aaaccaccca tcaatggcag cgcgacctgg ggctgcgccc
     5101 gaaagtcgtg gccccctggg cggtcagcac cgccccgccc cggcccaccg acccggtgac
     5161 cgtggccgat gccggcgata gccaggacat cgtgccggcg gtgcccagcc ccacgcccga
     5221 tctcaccgtg atccatgagt tgccggggga actgccgagc gagacgccca cgccggtgcc
     5281 ggaaacccgc gtcatcgacc tgctcccggc cacggtggcg ccgccgaccg ccaccccccc
     5341 gccgaccgcc actcgcccgc cgaaacgggc cacgcgcacg ccgcgcccgg tgaagcacac
     5401 ggccacgccg gtgcccaaac tgcccgcggc gcagcccacc gagccgccca ccgccgtcgc
     5461 ggtggagccg gcggccccgg cggtctcgcc ctcgccggtg atcgaggccg cgccgccgcc
     5521 ggtggcaccg cccccggtct acaccggtgc cggccccgcg cccatggcgc cgatcattca
     5581 ggacggcgcg ctgccgggcg agggcgtgtg gacggcggac ggtttcccgg ccgcgcccga
     5641 cggtggcccg cccgccttct ggaaaaccta cctgcgcccc gacgcggcgc gcccggatgc
     5701 catggtctac atggtgcgcg tcgatctccg ccgcgtccgg ctgcatatgg tggccggcac
     5761 caaagagccg gtctcgtcgc tgggcacgca cggcagcggc gtggtgccgc ccgccgatca
     5821 ggccctgctg gcgggtgcct tcaacggcgg ctggaagtcg gtccacggca actatggcat
     5881 gcgggtcaac ggcctgcaga tcgtgccgcc caacccgcgg cccgacacgg ccacgctcgc
     5941 cctgcacgcc gatgggcgca tggaactggc cggctggaag acgctgcaaa acgccggcga
     6001 cctggtgagc tatcgtcaga actgcccgct gctgatcgac aacggcacga tcaacgtctc
     6061 aacgcacatc acgagcacgt ggggcctgtc gctgctgaac gagatgtatg tctggcgctc
     6121 gggcgtggcg acgacgcccg acggcgcgct gatctacgcc gcgggcacgc ccatttccgc
     6181 cgatgaactg gccgccgcct tccagcacgc gggcgcgacc atggcgatgc aactcgatat
     6241 caacagcgcc tgggtacaat ggctgtcgta tgaccgcaac agcgcgggcc gcccgcaagc
     6301 gacgccgctg gtgcccgaga tggcctaccg gcgcgaccag tatctgtcgc cgaacgaccg
     6361 ggacttcttc tatctgacct ggcgcatgtc cacggcgcgg tagccttcgg ccggcacggg
     6421 ccggtttgaa accggcccct acaccgctac cggcgcaggc cgtggcgggg gcggaagata
     6481 cgcgggtcag tttgaaaccg gcccctacag ggctaacggt gcgggacgta gcgagggaag
     6541 gcggcacggg ccggtttgaa accggcccct acagggggca ttgtaaagcg ccctggtacg
     6601 catgatgatt gcgtgccggg gcgctttgtt gcctcgggtt ggctatcccg cgggccgggg
     6661 tatggtggcg gtggaggtcg ggcgggctag cgggtttcga ggcgccactg gccttcgccg
     6721 tggaccaccg agtggacgta atcggcgacg tgttcgcgct cttccacgcc gtccagcacc
     6781 atgcactcca gcaccggcac catgtccggg gcgacctcgt ccagttcctc gatcaccggg
     6841 cgatcgttgc gcgcgggcga ggggtcggtc acgtagtcat agagttcgtc ggtgagggcg
     6901 cgctcgttga cctcgatatg gaaaaagcga taattgttgc tcatggtttc aggctcctct
     6961 cagggcgcgg ggcgcccatc ggtgtggcaa gctaacgcga tgatggggcc ggcgcggcga
     7021 ccggccggtg ggcctcgata cagcggacgc cctgcggccc gaccgtggcc gcgtggcggg
     7081 tgttgggtgg cagcaacagc cg
//
LOCUS       DASUYC010000091         2135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_73159,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000091 DASUYC010000000
VERSION     DASUYC010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73159"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..1094
                     /locus_tag="VF276_01455"
     CDS             141..1094
                     /locus_tag="VF276_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEX6605555.1"
                     /translation="MITLDDIYAAREAIQGALVRTPLIFSEALSERVGRPLWLKLENL
                     QQTGSFKPRGVLTKIAALSAEEKERGLVTISAGNHAQALAYAARAAGVRCTVVMPADA
                     PATKIANTRAYGATVILHPDRVTLLDRCREEQEAHGYVYAPPFDDPQVIAGQGTVGLE
                     IVEDLPDVAAVVVPIGGGGLIAGVAVAVRGLRPEALVYGVEPEGAPGMVRSLAEGHAV
                     HLDKISTIADGLAAPFAGELNYAIVRDMVHQVVLLSDEEIAAAIPPLMEWAKVVPEPG
                     GAAALAGLLAGKLDLPAEGPVVAIVSGGNVDLSRLRGFLPA"
     gene            1150..>2135
                     /locus_tag="VF276_01460"
     CDS             1150..>2135
                     /locus_tag="VF276_01460"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX6605556.1"
                     /translation="MIAATPGQVLGGRYRLERLLGAGTVALVYEARDEQLERQVAVKI
                     LRPEWAQDPPTAAAFEREAQAGAGLHHPHLAQIYDAGSADGAPYIVMERVPGAPLDPD
                     RRLAPAQAVDLGAQIAGALAFVHDHGLLHCDIKPANLLVDPAGQVKLVDFGVACPVDE
                     PPPAAAPADAAMGTTLQLPAAHASPGMLPYLAPERLVGAPPCPAADVYSLGAILYTVL
                     AGRPPFPGTSADEIAAAQRAGAPPALRDLNPAVPPGLEAAVARAVAVDPAQRFATARE
                     MEAALEAVRAESRAQTAAVAAPPPVVFAPSDVTVANPVPPNAEQTVANAVPPS"
BASE COUNT          309 a          759 c          776 g          291 t
ORIGIN      
        1 ggagttagcg ggcgacgtgc tgcacgacgc tgtgataacg agacgcccgc tgacaaacgc
       61 tattattcct gacggctgat cgctgatagc tgacggctga cggctgacgg ctgacggcta
      121 atcgctaacg gagcagacat atgattacgc tagacgacat ctacgcagcg cgcgaggcga
      181 tccagggcgc gctggtgcgg acgccgctga tcttcagcga ggcgctgagc gagcgagtgg
      241 gccggccgct gtggctgaag ctggagaact tgcagcaaac cgggtcgttc aagccgcgcg
      301 gcgtgctgac caagatcgcg gcgctgagcg ccgaggagaa ggagcgcggc ctggtgacga
      361 tttcggcggg caaccatgcc caggcgctgg cctacgccgc gcgggccgcc ggagtgcgct
      421 gcacggtcgt catgcccgcc gacgcacccg ccaccaaaat cgccaacacc cgcgcctacg
      481 gcgcgacggt catcctgcac ccggatcgcg tgacgctgct ggaccgctgc cgggaggaac
      541 aggaggcgca cggctatgtc tatgccccgc cgttcgacga cccgcaggtc atcgccgggc
      601 aaggcaccgt cggcctggag atcgtggagg acctgcccga cgtggccgcg gtcgtcgtgc
      661 ccatcggcgg tggcgggcta atcgccgggg tggccgtcgc ggtgcgcggc ctgcggcccg
      721 aggcgctggt ctacggcgtc gaacccgagg gcgcgcccgg catggtgcgc agcctggccg
      781 aagggcacgc cgtccatctc gacaagatta gcacgatcgc cgacgggctg gccgcgccgt
      841 tcgccggaga gctgaactac gccatcgtgc gcgacatggt ccaccaggtc gtcctgctct
      901 ccgacgagga gatcgccgcc gccatccccc cgctgatgga atgggccaaa gtggtgccgg
      961 agccgggcgg cgcggcggcc ctggccggac tgctggccgg caagctcgat ctgcccgccg
     1021 aggggccggt ggtggcgatt gtcagcggcg gcaacgtcga cctcagccgc ctgcgcgggt
     1081 tcctgcccgc atgaggacca attgcgtaaa ctggcacagc ctgtgctttt acgggagcgg
     1141 aggttgttta tgatcgctgc aacgccaggg caggtgctgg gcgggcgcta ccggctggaa
     1201 cgtctgctgg gtgcgggcac cgtcgcgctg gtctacgagg cccgcgacga gcaactggag
     1261 cgccaggtgg ccgtcaagat cctgcggccc gagtgggcac aggacccacc gaccgctgcc
     1321 gccttcgagc gcgaggcgca ggccggggcc gggctacatc acccgcacct ggcccagatt
     1381 tacgacgcgg gctcggcgga cggcgcgccc tacatcgtca tggagcgcgt gcccggcgcg
     1441 ccactcgatc ccgaccggcg gctggcgccc gcgcaggcgg tggacctggg cgcgcagatc
     1501 gccggggcgc tggccttcgt gcacgaccac gggctgctgc attgcgacat caagcccgcc
     1561 aacctgctgg tggacccggc ggggcaggtc aagctggtgg acttcggcgt ggcctgtccg
     1621 gtggacgaac cgccgcccgc cgccgcgccg gcagacgccg ccatgggcac gacgctgcaa
     1681 ttgcccgcgg cgcacgccag tccgggcatg ttgccgtacc tggcgccgga gcggctggtg
     1741 ggcgcgccgc cctgccccgc cgccgatgtc tacagcctgg gcgcgatcct ctacaccgtg
     1801 ctggccgggc gcccgccttt ccccggcacc agcgccgacg agatcgccgc cgcccagcgc
     1861 gccggggcgc cgcccgccct gcgcgacctg aaccccgccg tgccgcccgg tctggaggcc
     1921 gctgtggccc gcgccgtggc ggtcgatccg gcgcaacgct ttgccaccgc gcgtgagatg
     1981 gaagccgcgc tggaggccgt tcgcgcggaa agccgggcgc agaccgccgc cgtggccgcc
     2041 ccgccgccgg tcgtcttcgc gccgtccgac gtcaccgtcg ccaacccggt gccgcccaat
     2101 gcggaacaga cggtggcgaa tgcggtgccg ccgtc
//
LOCUS       DASUYC010000092         2636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1228414, whole genome shotgun sequence.
ACCESSION   DASUYC010000092 DASUYC010000000
VERSION     DASUYC010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228414"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..454)
                     /locus_tag="VF276_01465"
     CDS             complement(134..454)
                     /locus_tag="VF276_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605557.1"
                     /translation="MTADPRAAYDAVAAELAGAGVSSSKMFGMPCLKYGGKAFAGFHQ
                     EAMTFKLRLPAHAAALALPGAHLFDPSGAGRPMREWVEVPATHAARWPEFAREALHYL
                     ESTL"
     gene            complement(590..1660)
                     /locus_tag="VF276_01470"
     CDS             complement(590..1660)
                     /locus_tag="VF276_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605558.1"
                     /translation="MKSPLKSRRPLGSLFILLLVLPAVISWPAAAQDSSRTFPETGQT
                     VSGPFLAYWTAHGGLAQQGYPISEELLEVSDLDGKPYRVQYFERAVFEQHPENQPPFD
                     VLLSQLGTFQYKAKYGAAGAPGQVAGTTNPRLFPETGKTLAGTFRAYWEQHGGLAQQG
                     YPISNEFQETSPLDGKVYTVQYFERAVFELHPENAPPFDVLLSQLGTFRYQAKYQATP
                     TATALPTRTAAPAPTNTPVPTAPAGPVTTCTDIPPSQNMRVVPNCIPLFEQVGLIGEK
                     FQGDNEVGVYATMPNGQVFGAPFHVHVWTTGETQLVHLIVDDQTPYFGIWTVTMEGVQ
                     THRKAFGYFRLIPQATPAPGRP"
     gene            complement(2001..>2636)
                     /locus_tag="VF276_01475"
     CDS             complement(2001..>2636)
                     /locus_tag="VF276_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605559.1"
                     /translation="WRTGRDWLRRRPPLTPAVALLVLTTLGHIVLIPASITYHDTYVL
                     PFLPGLIGMLLLGARLTRPAVGLAGAGLAAFVVISLLQMGEYKGWQQAATAAEAALIR
                     QGVPRDHILGTAEWAYWDLWETDARAAKAAGEPRPPEFQPGQVIPDFVISYVPQPREF
                     FILFALPPEDVAAHYTICARTPYAQPFAAAPAEIYTLTRTRCPAGSAQAAP"
BASE COUNT          420 a          856 c          947 g          413 t
ORIGIN      
        1 gggcgggcta cgtcaacgaa ggccgcctgc gcggcctgga tcatgttggt cccagcccgc
       61 gcaggcgggc ttggtggtgc tagcccgctc ctttaggagc cgggcggtgc cgcgctcgtg
      121 gccgggccgg gcctcacagg gtagattcca ggtagtgcag ggcctcgcgg gcgaactcgg
      181 gccagcgggc ggcgtgcgtg gcgggcacct cgacccactc ccgcatcggg cgacccgcgc
      241 cggaggggtc gaacaggtgg gcgccgggca gggccagcgc ggcagcatgc gcgggcaggc
      301 gcagcttgaa ggtcatcgcc tcctggtgga acccggcaaa ggccttgccg ccgtatttga
      361 ggcagggcat gccaaacatc ttgctgctgc tgacgccggc cccggccagc tcggcggcga
      421 cggcgtcgta ggcggcgcgc ggatcggcgg tcatgggcgt ggtcctcctc tcctgtggca
      481 cactcatacg gtcgcgattc tattatctgt ccgccgtcaa taggccccgg cgcccacaaa
      541 aaacccagcc tgttcgcccc agccacagag tgtagccgga acggcaaggt cagggccgcc
      601 cgggcgcggg cgtcgcctgc gggatcagcc ggaagtagcc gaaggctttg cggtgcgtct
      661 ggacgccctc catcgtcact gtccagatcc caaagtatgg tgtctggtca tccactatca
      721 gatggacaag ctgcgtttcg cccgtggtcc acacgtgaac atggaagggc gccccaaaca
      781 cctggccgtt gggcatggtt gcgtagaccc cgacctcatt atctccctgg aacttctcgc
      841 cgatcaaacc aacctgctca aacaagggga tgcaattcgg caccacgcgc atattctggc
      901 tgggcgggat gtcggtgcag gtggtgaccg ggccggcggg cgcggtgggc acaggcgtgt
      961 tagtcggcgc gggcgccgcc gtgcgggtgg gcagggccgt agccgtgggc gtggcctggt
     1021 acttggcctg atagcggaag gtgcctaact ggctcagcag cacgtcgaac ggcggggcat
     1081 tctccgggtg caactcgaac accgcccgct cgaaatactg cacggtgtag accttgccgt
     1141 ccaggggcga ggtctcctga aactcgttgg agatggggta gccttgctgg gccagcccgc
     1201 cgtgttgctc ccaataggcg cggaacgtgc cggccagcgt cttgccggtc tccgggaaca
     1261 ggcggggatt ggtcgtgccg gcgacctggc cgggcgcgcc ggcagcgccg tatttggctt
     1321 tgtactggaa ggtgcccaac tggctcagca acacgtcgaa gggcggctga ttttcgggat
     1381 gctgctcgaa cacggcccgc tcaaagtact gcacgcggta cggcttgccg tcgaggtcgc
     1441 tgacctccag cagttcctcg ctgatgggat agccctgctg ggccagcccg ccatgcgcgg
     1501 tccaatacgc cagaaacggg ccgctcacgg tttggccggt ttcggggaag gtgcggctac
     1561 tatcctgcgc ggcagccggc caactgatga cggccggcag caccagcagc aggatgaaca
     1621 ggctgccgag gggccggcga cttttcaagg ggctcttcaa cgcgtacgct cctccgtgtg
     1681 gttgggtctt gggccaataa ggtcgatgca ggaagccggc ggcctccggt ggtaaaaggc
     1741 agccggacca ggactcattt acccctggag atgggccgat tcccgccgct cgctattata
     1801 gctggtctcg tccgtttgcg ccaccggcct ggcactggat ggaaagccgc cgccccaccg
     1861 gcccgtgggg aacccggccc ccggtgcccg gcgtctatag catagcaggc atccggcggc
     1921 acgagcggac ccttccagaa cctcgtgccg cccgatgcgg tatccgccgg acgccgggag
     1981 cgcacgcccg gccacgcgtg tcacggcgcc gcctgggcgc tcccggcggg acagcgggtg
     2041 cgggtcagcg tgtagatctc ggcgggcgcg gcggcgaacg gctgcgcgta gggcgtccgg
     2101 gcgcaaatgg tgtagtgggc cgccacatcc tcgggcggca acgcgaacag gataaagaac
     2161 tcgcgcggct gcgggacgta gctgatgaca aaatcgggga tcacctggcc gggctgaaac
     2221 tccggaggcc ggggctcgcc cgcggctttc gcggcgcggg cgtccgtctc ccagaggtcc
     2281 cagtaggccc actcggcggt gccgagaata tggtcgcggg ggacgccctg ccggatcagg
     2341 gcggcttccg ccgcggtcgc cgcctgctgc cagcctttgt actcgcccat ctggagcaga
     2401 ctgatcacga caaaggccgc caggccggcc ccggccaggc caaccgcggg gcgcgtcagg
     2461 cgcgcgccca gcagcagcat cccgatcagg ccgggcagga acggcagcac ataggtgtcg
     2521 tgataggtaa tgctggccgg gatcaacacg atgtggccca gggtggtgag caccaggagc
     2581 gcaaccgcgg gggtgagcgg cggccgccgg cgcagccagt cgcgcccggt gcgcca
//
LOCUS       DASUYC010000093         2937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_997565,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000093 DASUYC010000000
VERSION     DASUYC010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997565"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            484..1521
                     /locus_tag="VF276_01480"
     CDS             484..1521
                     /locus_tag="VF276_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605560.1"
                     /translation="MRSTEPHLSLSCRPRRPAVLTLVSLACLTLAAVLSVPLAVPAGA
                     APLPTSGSIYFSQTNHSASNGFLSFWLAHGQAERWGYPITDEFKENGVVVQYFERGRL
                     EYHPEAPANARITASLTGSLLVGSRRFQPGPAIRPTSYETYFPQTQHTLRGSFYNYWR
                     SHGDLASFGYPISEEFVEGGHTVQYFQRARFELTRDGGIWLGHIGRELLDLHNVEAAA
                     QAAARPGFEMSFNGGSTWFPGNWQRIISLNQSWGNLPYGYKGYGFYAAMPADLNLYGR
                     WGRVTRGDKSIWVQFVDVIDWKDINQVRADGKVIDLGTESFQQFAPLSAGVIRVTVDV
                     AWPGFQPEFAR"
     gene            1690..2898
                     /locus_tag="VF276_01485"
     CDS             1690..2898
                     /locus_tag="VF276_01485"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEX6605561.1"
                     /translation="MRTTAAPNRSLPFVDWYLLPESYSTQLVNQYIDRYAIPPGATVL
                     DPFAGAGTTVVAARLRGLHALGVEINPFLAFAARVKSRFGYDLPRLQADVDALLARVD
                     AALAGPVPDYPLPAMPRLERWIYPHVARRLLSVRAAILEIAEPAHREFCLLAFAAILR
                     RCSNMKLLPHSFGTRLDRSAPPIRAMLAAHLAKMLADVAGLPVPPEGCGEIIVGDARA
                     LDAPGHPLLPAALAVTSPPYLNNLDYTMQTRLELFFLGFVDDIADVRRIRKRMLVCDA
                     KAVYPEIEDYQLVTDHAEVAGLVDALREKHAAKNWGWDYPLMVGQYFGGMRRVLAGVH
                     RALAPGGRLVLITGESAHSGILVPVPRLTAELGELTGFRVESIQVLRTRRSPSHGLPI
                     AESAVTLVRE"
BASE COUNT          444 a         1030 c          943 g          520 t
ORIGIN      
        1 ccctgcccgg cagcagcgcc ggaacgccgt ctacgtcaca ggtaggtcaa tagctggtaa
       61 tctcctacgc agaagaagtg ccttaactta cccccacttt actcgcacct taccgccaac
      121 ttacagcctg ttttgtgaca caaatgttat gctctccccg ttgctattgc tttgccggtt
      181 ttggtacaat ccgctttgtg cgtacccctc acgccgaacg gtaaggattg gccctaacct
      241 caggtgagtt aaatacgcat ccgtcagccc cgccgccacg tgtgcggacc gtcactccgc
      301 gcgtgggtca tcggtgctag ggcgggcggg atccaggctc ggtgactggc gaacgccggg
      361 ctttgctatg tcgcacgcct gttgccctgg gggcgctgag gccaattcag cgccgcgcct
      421 tgcggcggtt tcccgcaaga ccgggccggg ccggtgccta ttcgaaggag tagtgttcgc
      481 tatatgcgta gtacggaacc ccatttatcc ctgtcgtgcc gccctcgtcg tcccgcagtt
      541 ctcacccttg tcagtctcgc ctgtctcacc cttgccgccg tgctgagcgt accgctcgcc
      601 gtgcccgcgg gcgccgcgcc actgccgacc agcgggtcga tctatttcag ccagaccaac
      661 cactcggcca gcaacggctt cctgagcttc tggctggcgc acggccaggc cgagcgctgg
      721 ggctacccca tcaccgatga gttcaaggag aacggcgtcg tcgtgcagta tttcgagcgc
      781 gggcgcctgg aataccaccc cgaggctccg gccaatgcgc gcatcacggc cagcctcacc
      841 gggagtctgc tggtgggcag ccgccgcttc cagccagggc cggcgatccg ccccacgagc
      901 tacgagacct atttcccgca aacccagcac acgctgcgcg gcagcttcta caactactgg
      961 cgcagccacg gcgacctggc ctcgttcggc tacccgatca gcgaggagtt tgtggagggc
     1021 ggccacacgg tgcagtattt ccagcgcgcc cgctttgaac tgacccgcga cggcggcatc
     1081 tggctgggcc atattggccg cgaactgctc gacctgcata acgtcgaggc agcagcccag
     1141 gcggcggccc gccccggctt cgagatgtcg tttaacggcg gctcgacctg gttccccggc
     1201 aactggcagc gcatcatcag cctgaaccag agctggggca acctgcccta cggctacaag
     1261 ggctacggct tctatgccgc catgcccgcc gacctgaacc tgtacgggcg ctggggtcgc
     1321 gtgacccgcg gcgacaagag catctgggtc cagttcgtgg atgtaatcga ctggaaggac
     1381 atcaaccagg tgcgcgccga cggcaaggtc atcgacctgg gcaccgagag cttccagcag
     1441 ttcgcgccgc tcagcgcggg cgtgatccgg gtgacggtgg acgtggcctg gccgggcttc
     1501 cagcctgaat ttgcgcgcta gccgtctgct ctatgggcgc gggccggttt caaaccggcc
     1561 cctacaacgt gattgaggcg cgggccgggg agattgcctc cccggcccgg ttgctttccg
     1621 gcggcccgcc ctgcccggca tcgcgatcat cgtgtataat gagcagcaaa tcgccggagg
     1681 ccctgccaca tgcgcactac cgccgcgccc aatcgctcgt tgccgttcgt cgattggtat
     1741 ctgctgccgg aatcgtattc cacccaactc gtcaaccagt atatcgaccg ctacgcgatc
     1801 ccgccgggcg ccacggtgct cgacccgttt gccggggccg ggacaacggt ggtggctgcg
     1861 cgcctgcgcg ggctgcacgc cctgggcgtg gagatcaacc ccttcctggc cttcgccgcg
     1921 cgggtcaaaa gccggttcgg ctacgacctg ccgcgcctgc aggccgatgt agatgcgctg
     1981 ctggcgcggg tcgatgccgc gctggccggg ccggtgcccg actacccgct gcccgccatg
     2041 ccgcgcctgg agcgctggat ctatccgcac gtggcccggc ggctgttgag cgtgcgcgcg
     2101 gctatcctgg agatcgccga gcccgcgcac cgcgagttct gcctgctggc gttcgcggct
     2161 atcctgcgcc gctgctcgaa catgaagctg ctgccccatt ccttcggcac gcggctcgac
     2221 cgcagcgccc cgcccatccg cgccatgctg gcggcgcacc tggcgaagat gctggccgac
     2281 gtggccgggc tgcctgtgcc gccggagggc tgcggcgaga tcatcgtcgg cgatgcgcgc
     2341 gccctcgacg cgccgggcca ccccttgctg cccgccgcgc tggccgtcac ctcgccgccc
     2401 tatctgaaca acctcgacta caccatgcag acccggctgg aactgttctt cctgggcttt
     2461 gtggacgaca tcgccgacgt gcgccgcatc cgcaagcgca tgctggtgtg cgacgccaag
     2521 gccgtctacc cggagatcga ggactaccaa ctggtgacgg accacgctga ggtggcgggg
     2581 ctggtggatg cgctgcgcga gaagcacgcg gcgaagaact ggggttggga ctacccgcta
     2641 atggtggggc agtatttcgg cggcatgcgg cgggtgctgg cgggcgtgca tcgcgccctg
     2701 gcgccgggcg gccgcctcgt gctgatcacc ggcgagtcgg cgcacagcgg catcctcgtg
     2761 ccggtgcccc gcctcaccgc cgaactgggc gaactgaccg gcttccgcgt ggagtcgatc
     2821 caggtcctgc gcacgcggcg cagcccctcc cacggcctgc ccatcgccga gtcggcggtc
     2881 acgctggtgc gggagtaggc gtcgctaaat cgcgggaaac cagagcaggg cttcgtc
//
LOCUS       DASUYC010000094         5411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1921483, whole genome shotgun sequence.
ACCESSION   DASUYC010000094 DASUYC010000000
VERSION     DASUYC010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5411
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1921483"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1696)
                     /locus_tag="VF276_01490"
     CDS             complement(<1..1696)
                     /locus_tag="VF276_01490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX6605562.1"
                     /translation="MTLDLAPAPITAGDIRSPERGLSESEVRARRAAGQGNNAPLTTS
                     RTYPEILRAQAVSFINIVLFCIGLGLVLLGRPDDAFMTAGIVLLNVIVGVGQEVRAKR
                     QLDHIALLTRPQATVIREGREQSVDPSALVLGDLLIVRPGDQIVLDGTVVRGGRMEVD
                     ESLLTGESDRVPKQAGDAVYSGSFCVTGSARYVARQVGAGSLANQITASARAFRQVKT
                     PLQCDVEFVVRLLVVLVLLLGLLLGLAYLINPIPVVQQVQIAAVLVALVPQGLFAMIT
                     VTYAMGALRMAGKGALIQQINAVESLSNVTVLCLDKTGTLTSNTLKLADLVPLGITEQ
                     ELTCLLGDYVASISAGNRTAEALGAACAGQARPVREEVVFSSAQKWSAVIFAGGAGPG
                     VYVLGAPEILEPNLQPGSDLGPRPATWADQGLRVLLFAYCADPVELRGPDGEPVLPGG
                     LIPLGLVSLRDELRAEAHNTLQAFAAAGVHLKIISGDHPQTVAALARQAGLGVDHGDL
                     QVVSGLDLADMDEAAFARAAAEATIFGRITPAQKEHLVRCLQAQGHYVAMIGDGVND"
     gene            1973..2674
                     /locus_tag="VF276_01495"
     CDS             1973..2674
                     /locus_tag="VF276_01495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019586959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6605563.1"
                     /translation="MIYSDLELARMQVEALFVHDAQGRLLRVNEQDPGEPAPRFFLAR
                     TATGNLWRTRADLPADLSAALVRLAADEPIAADLREPAAHVAEYAALLEQHTPISHTE
                     AGPAYYLPELDPPACAVIITPENANLVEAHFPYTRTHYAELAPVVVCVADGVAVAVCF
                     SARITARVAEAGVYTVEAYRGRGYAADTVRGWAAAVRALGRLPLYSTSWDNTASQAVA
                     RRLGAVQYGVDLSIM"
     misc_binding    2756..2996
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3066..4328
                     /locus_tag="VF276_01500"
     CDS             3066..4328
                     /locus_tag="VF276_01500"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX6605564.1"
                     /translation="MDNKLAVLAFSGGLDTSFCVPWLQEQGYDVVTVTVDTGGFSAAE
                     RAAIEARATALGVVAHHTIDGRQPLWDLLVGYIIKGNVLRGGVYPLCAGPERLVQAMQ
                     VVGVAQSYGTTTIAHGSTGAGNDQVRFDLAIRVLLPEANILTPIRDLNAQRAFEVEFL
                     NKHGIPVPEKTGRYSVNKGLLGTTIGGGETLDSWGFPPDEAFVDTVAPADAPDEPAVI
                     TLSFADGVPVALDGVAMAPLDLMAALAEIGGKHGVGRGLHLGNTILGLKGRIVFEAPA
                     AVIAITAHKELEKLVLSKNQGFWKDHLADVYGNMLHEGLYFDPVMRDIEAMIDSSQGA
                     VTGDVRVSLFKGHIQVQGCRSPYSLLDRKVGTYGEANRAWTGAEAAAFCKLYGLQSML
                     AYRRQHTPVPAPDGKLHAILNMPAGAKV"
     gene            5026..>5411
                     /locus_tag="VF276_01505"
     CDS             5026..>5411
                     /locus_tag="VF276_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605565.1"
                     /translation="MQVTLDKIASVTARVNLSREVEVSSSVVAERGAVIIVEALEEKS
                     VYGELELLGGRMARIIKGDIIAGVLGERQALKGFVGAIPGQIAAGDILHILNLGGVIG
                     VCSSANAEVGQPLRVRVLGAALVDGQP"
BASE COUNT          972 a         1731 c         1843 g          865 t
ORIGIN      
        1 cgtcgttgac gccgtcgccg atcatggcga catagtggcc ctgcgcttgg aggcagcgga
       61 ccagatgttc tttctgcgcg ggcgtgatcc gcccgaagat cgtcgcttcc gcggcggcgc
      121 gggcgaacgc ggcctcgtcc atgtccgcca gatccagccc cgaaaccact tgcagatcgc
      181 cgtggtcaac gcccagcccg gcctgccgcg ccagggcggc gacggtctgc ggatgatcgc
      241 cggagatgat cttcaggtgg acgcccgccg ccgcgaaggc ctggagcgtg ttgtgggctt
      301 cggcccgtaa ctcgtcgcgc aagctcacca accccagggg gatcaggccg ccgggtaaga
      361 ccggctcgcc atcgggcccg cgtaactcga caggatcggc gcaataggcg aacagcagca
      421 cgcgcaagcc ctggtcggcc caggtggcgg gccgcgggcc gagatcgcta cccggctgca
      481 aattcggctc caggatctcc ggcgcaccga gcacgtagac gccgggcccc gcgccgccgg
      541 caaagatcac cgccgaccac ttctgcgccg acgagaagac cacttcctcg cgcaccgggc
      601 gggcctgccc ggcgcacgcg gcgcctagcg cctcggcggt gcgattgccg gcggagatgc
      661 tggcgacgta atcgcccagg aggcaggtca gttcctgctc agtgatgccc agcggcacca
      721 ggtccgcgag cttgagcgtg ttgctggtca gggtgcctgt cttgtccagg cacagcaccg
      781 tcacattgct caaggactcg acggcgttaa tctgctggat cagcgcgcct ttgcccgcca
      841 tgcgcagggc gcccatggca taggtcacgg tgatcatggc gaacagcccc tgcggcacca
      901 gcgcgacgag cacggcggcg atctgcacct gctgcaccac ggggatcgga ttgatcaggt
      961 aggccaggcc gagcaatagc cccaatagca gcaccagcac caccagcaac cgcacgacaa
     1021 actcgacatc gcactgcagc ggcgtcttga cctggcggaa agcccgcgcg ctcgccgtga
     1081 tctggttcgc caggctgccc gccccaacct ggcgcgcgac atagcgcgca ctgcccgtga
     1141 cgcagaagct gcccgagtaa accgcatcgc cggcctgctt gggcacgcga tccgactcgc
     1201 cggtcagcag cgactcgtcg acctccatgc gcccgccccg gacgaccgtg ccgtcgagca
     1261 cgatctggtc gccgggccgc acgatcagga ggtcgcccag caccagcgcg ctcgggtcca
     1321 cgctttgctc gcggccctcg cggatgaccg ttgcctgggg tcgggtgagc agcgcgatat
     1381 ggtcgagttg ccgcttcgcg cgtacctcct ggcccacccc aacgatcacg ttgagcaaga
     1441 cgatgccggc cgtcatgaag gcgtcgtcgg gccggcccag cagcaccagg ccgaggccga
     1501 tgcagaacag cacgatgttg atgaaggaga cggcctgggc gcgcaggatc tccggatagg
     1561 tgcgactggt ggtgagcggt gcgttgttgc cctggcccgc ggcgcgccgg gcgcggacct
     1621 cggactcgga taagcctcgt tccggggagc gaatatcgcc ggccgttatc ggggccggcg
     1681 caagatcgag ggtcatcggc gatctcccta ttctagtctg cgtccgacac cgccggatga
     1741 ccggcgtgat cgccatgcca acattgccga gtctagtatc ggtcgcccag gtatataagg
     1801 cggcatatac cgggcaacaa caggtaaaaa gcccgcgggg cgccgggctt gcctgccctg
     1861 tgaccgccca tcgcacacgc ctggaaccca atcggcggca aatgcgcgcc gatccggcct
     1921 cgacagcgcc ggtatcctgt gctacaatag cgaattcgga gaaagcgcac ctatgatcta
     1981 cagcgatctt gaactggccc ggatgcaggt ggaggcgctc ttcgtgcatg acgcacaggg
     2041 gcggctgctg cgcgtcaacg agcaggatcc cggcgagccg gcgccgcgct tctttctggc
     2101 ccgcaccgcg accggcaacc tctggcgcac gcgggccgat ctgcccgccg atctgagcgc
     2161 ggccctggtc cggctggcgg cagatgagcc catcgccgcc gatctccgcg aaccggccgc
     2221 gcacgtggcc gaatatgccg ccttgctgga gcaacacacg cctatcagcc acactgaggc
     2281 gggaccggcc tactatctgc cggaactcga tccgcccgcc tgcgcggtga ttatcacgcc
     2341 ggaaaacgcg aacctggtgg aagcccattt cccctacacg cggacccact acgccgagct
     2401 agcgccggtg gtggtgtgtg tggccgatgg ggtggcggtg gccgtgtgtt tctcggcgcg
     2461 gatcaccgcg cgggtggcgg aagccggggt ctacacggtc gaggcgtacc gcgggcgcgg
     2521 ctatgcggcg gacacggtgc gcggctgggc cgccgccgtt cgcgccctgg gccggctgcc
     2581 gctctacagc acgtcctggg acaacaccgc ttcgcaggct gtggcgcggc ggctgggggc
     2641 cgtgcagtac ggggtcgatt tgagcatcat gtaatttggt tgcgatcaag ccttgacatg
     2701 cgcgacaggt tgtgctacaa tagcccatgc aaattgaata gcggccgttg gccgcgtgcc
     2761 cgcgttgatg tggaatagta gttccaatgc gtgtttgcag cgaatcgggg gttggtgtga
     2821 gcccgagaaa ccagttggag tgaacgcgcc acagagcggt ccaccgaaga aagcccaatg
     2881 gcgggcaagt aggatggagt gggtggtgcc cacgccggca acaaccaaga gcggtcacag
     2941 gctataaatg tgacaattag ggtggtacca cgggagtctg gactctcgtc ccttgctttc
     3001 gatagcggga cgggagtttt ttattgccca aaattggggt cagacggccc ctggaggata
     3061 cgacgatgga caacaagctc gcggtgttgg cgttttcggg cggcctggat acttcgttct
     3121 gcgtgccctg gctgcaagag caaggctacg acgtggtgac ggtgacggtg gacaccggcg
     3181 gcttcagcgc cgccgagcgc gcggccatcg aggcgcgggc cacggccctg ggcgtcgtcg
     3241 cgcaccacac catcgacggg cgccagccgc tctgggatct gctggtcggt tatatcatca
     3301 agggcaacgt gctgcgcggc ggcgtctatc cgctgtgcgc cgggcccgag cgcctcgtcc
     3361 aggccatgca ggtggtcggc gtggcccaga gctacggcac caccaccatc gcccacggct
     3421 cgaccggcgc gggcaacgac caggtccgct tcgacctcgc catccgcgtg ctgctgccgg
     3481 aagccaatat cctgaccccg attcgagacc tgaacgccca gcgcgccttc gaggtcgagt
     3541 tcctgaacaa gcacggaatc cccgtgccgg agaagacggg ccgctattcg gtgaacaagg
     3601 gcctgctcgg cacgaccatc ggcggcggcg aaacgctcga ctcctggggc ttcccgcccg
     3661 atgaagcctt cgtggacacg gtggccccgg cggacgcgcc cgacgagccg gcggtgatca
     3721 cgctcagctt cgccgacggc gtgccggtgg ccctggacgg cgtggcgatg gccccgctcg
     3781 acctgatggc cgcgctggcc gaaatcggcg gcaagcatgg cgtgggccgc ggcctgcacc
     3841 tgggcaacac gatcctgggc ctcaagggcc gcatcgtctt cgaggcgccc gccgcggtta
     3901 tcgccattac ggcgcacaaa gagctggaaa agctcgttct aagcaagaac cagggtttct
     3961 ggaaggatca cctggccgac gtgtacggca acatgctgca cgagggcctg tacttcgacc
     4021 cggtgatgcg cgatatcgag gcgatgatcg acagcagcca gggcgccgtg accggcgacg
     4081 tgcgggtgag cctgttcaag ggccacatcc aggtgcaggg ttgccgcagc ccctattcgc
     4141 tgctcgaccg caaagtcggc acctacggcg aggccaaccg cgcctggacc ggcgccgagg
     4201 cggcggcctt ctgcaagctc tacggcctgc aaagcatgct cgcctatcgc cggcagcaca
     4261 ccccggtccc cgcgcccgac gggaagctgc acgcgatctt gaacatgccg gcgggggcca
     4321 aggtctagcg agtgctgagt gctgagtacg acgagtggtc agtggtcagt ggtcagtggt
     4381 cagtagtgtt tgtcattctg agcggagcga agaatctccg tctggtaggc taaaaagacg
     4441 agactcttcg ctgcgctcag agtgacaagt gggggcgacc cggacgagac tcttcgctgc
     4501 gcttagagtg acaagccgga cgcagggact cagcactcag cacccagtac tcagcactcg
     4561 gtttggggta ttgacttatt tcgcgcggta tggtacactg cgccttaatt gaatagcggc
     4621 aacgagccgc gtgcccgcgt tgaagtggaa tagtacccgt agcaggggtc gacagcgaat
     4681 cagggagagt gcgagcctga gaggcctggt tacggcgaac ccgtcactga gcggtccgcc
     4741 gaagaaagcc caatgggggg caagtaggag ggagtgggtg gtgcccacgc gccgaacaac
     4801 cacgagcggt cacaggccag aaatgtgaca attagggtgg taccacggga gtgtgaactc
     4861 tcgtcccttg ctttcaggag cgggacggga gttttttatt gcccggaaac caggacgacg
     4921 gagtccggtg agcgtccagc gttggtcgca tttgagcaag acgatacgca gcgtaccata
     4981 cgaaaccggc tcgtggcggg ccgggtgggc gagaggagta acacaatgca agtcacgctg
     5041 gataaaatcg cgtcggtcac ggcgcgggtc aacctcagcc gcgaggtcga agtgagcagc
     5101 agcgtcgtcg ccgagcgcgg cgccgtcatc atcgtcgagg cgctggaaga aaagagcgtc
     5161 tacggcgaac tcgaactgct gggcgggcgc atggcccgga tcatcaaggg cgacatcatc
     5221 gccggggtgc tgggcgagcg gcaggcgctc aagggcttcg tcggggctat tcccggccag
     5281 atcgccgcgg gcgatatcct gcacatcctg aacctgggcg gcgtcatcgg cgtgtgcagt
     5341 tcggccaacg ccgaggtcgg ccaaccgcta cgggtgcgcg tattgggcgc ggcgctggtg
     5401 gacggccagc c
//
LOCUS       DASUYC010000095         2128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1624520, whole genome shotgun sequence.
ACCESSION   DASUYC010000095 DASUYC010000000
VERSION     DASUYC010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1624520"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2128)
                     /locus_tag="VF276_01510"
     CDS             complement(<1..>2128)
                     /locus_tag="VF276_01510"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6605566.1"
                     /translation="QRPSHQLAGLLVAALSVPPAQHAEVIHRLRIAPPTPAPTPVAAS
                     TGRDAGGRRLPNPLTALIGREADVGSVGAMLLKAEARLVTLVGPPGIGKTRLALAVAR
                     HLHEEFADGVWFVELAPLTDPALVVPTLLTTLGLPERRSKALLTYLTGFLQDKQMLLV
                     LDNFEHLLTAAAVVDALLKAAPALKVLATSRTPLRVYGEKEFPVPALRVPPDTQAAAN
                     DLGQYEAVRLFVERARDVLPDFALGAANAAAVAAICARLDGLPLAIELAAARIRLFPP
                     AALLARLDHRLPLLTGGARTLPARQQTLRAAIAWSYDLLDAGEQRLFRRLGVFQGGAT
                     LEAIAAICNADGDRDVLAGVEALVIQSLLQQREGRNGEARFWMLETLKEYAGEKLAAS
                     GEGPLLCEQHLSYFLTLAETAAPELRGPQQVAWGHRLDAEHDNLRAALRWAIDNLETE
                     TGLRLAGALFWFWYAHDAFSEGRAWLANLLALPASQGATAVARIKALNAAAALASRQN
                     DAASAMPLLKASLALAREVGDDMGIAWALHNYAMIAHYQGHYTQAVALSEQSLSLYRQ
                     MGDSWGIAASLLNLGNVMMSQGNYEQATARYDECLKVCRQAGNKRGMAFTLHQQGLVA
                     QHQGDYDRASALLGKSLLLFREIGEVWGIAWSGTVLGRVALHQGDYEQAAVALEEGLA
                     AARKLDDKKDKAFALIVLGNRAFKQG"
BASE COUNT          356 a          730 c          685 g          357 t
ORIGIN      
        1 ttgccttgtt tgaaggctct attccccaac acgatgagtg caaaggcctt atccttcttg
       61 tcgtctaact tccgagccgc cgccaggcct tcctccagcg caactgccgc ctgctcatag
      121 tcgccctgat gcagcgccac acgcccgagc acagtaccgg accacgcaat accccatact
      181 tctcctatct cgcggaacag aagcaaactc tttccgagca gcgcactcgc tcgatcgtag
      241 tcgccctggt gctgggccac taacccttgc tggtgaaggg tgaaagccat cccgcgcttg
      301 ttccccgcct gccggcagac cttgaggcac tcgtcataac gcgctgtcgc ctgttcatag
      361 ttcccctgac tcatcatcac gttccctaag ttcaagagcg acgcagcgat accccagctg
      421 tcgcccattt ggcgatacaa gctgaggctc tgctcagaca gggcaacggc ctgagtatag
      481 tggccctggt agtgtgctat catcgcataa ttgtggagcg cccaagcgat ccccatgtcg
      541 tccccgacct cgcgggccag agccaggctg gctttgagca aaggcattgc cgaagcagcg
      601 tcgttctgcc tggacgccag cgcggcggct gcattgaggg ccttaatccg tgcgaccgct
      661 gtggcgccct ggcttgcagg gagtgccagg agattcgcca gccatgctcg tccctcactg
      721 aaagcatcgt gcgcgtacca gaaccagaat aaggctccag ccagtcgtaa ccctgtttct
      781 gtctccagat tatcgatcgc ccacctcagc gctgcccgca gattgtcgtg ctcggcatcc
      841 agccgatgcc cccaggctac ctgctgtggt ccgcgcaact ccggtgcggc agtctcggcg
      901 agcgtcagaa agtaggacag gtgctgttcg catagcagcg gcccttctcc actggccgcc
      961 agcttctccc cggcgtactc cttgagcgtc tccagcatcc agaagcgcgc ttctccgttg
     1021 cgcccctcac gctgttgcag caagctctgg atgaccagtg cctcgacccc agcgagcacg
     1081 tcccgatcgc catccgcgtt gcagatggct gcgatggctt ccagcgtcgc cccgccctgg
     1141 aacacgccca gtcgccggaa caaccgctgc tcccctgcgt ccaacaggtc gtagctccag
     1201 gcaatggccg cgcgcaacgt ctgctggcgg gccggtaggg tgcgcgcgcc cccggtcagc
     1261 agtggcaacc ggtggtccaa ccgggccagc aaggctgctg ggggaaagag ccggatgcgc
     1321 gccgcggcca gctcgatcgc caggggtagc ccgtccagcc gggcgcagat ggcggccacg
     1381 gcggcggcgt tcgccgcccc cagggcgaag tcagggagca catcgcgcgc ccgctccacg
     1441 aacaggcgca ccgcctcgta ctgccccagg tcgttcgccg ctgcctgcgt gtcgggcggc
     1501 acgcgcaggg cggggaccgg gaactccttc tcgccgtaaa cgcgcagtgg cgtgcggctg
     1561 gtcgccagca ccttgagtgc aggcgcggct ttcagcagcg catccacaac cgcggcggcg
     1621 gtcagcaggt gctcgaagtt gtccagcacc agcagcatct gcttgtcctg gagaaagccc
     1681 gtcagatagg tgaggagggc cttactccgg cgctcaggca ggccgagcgt ggtgaggagc
     1741 gtgggcacga ccagggcggg gtcggtgagg ggggccagtt ccacgaacca gacgccgtca
     1801 gcaaactcct cgtgcaggtg ccgggcgacg gccagggcga ggcgggtctt gccgatgccg
     1861 ggcgggccga ccagcgtaac tagccgcgcc tcggccttca gcaacatggc acccacgctc
     1921 cccacgtcgg cctcgcggcc aatgagggcc gtgagggggt tgggcagacg gcgcccgccc
     1981 gcatcgcgcc cggtggaggc ggcaacgggg gtgggcgccg gggtaggagg ggcgattcgc
     2041 aggcggtgga tgacctcggc gtgctgcgcg ggcggtacgc tcagggccgc gaccagcaag
     2101 ccggccagtt ggtgggacgg gcgctggc
//
LOCUS       DASUYC010000096         5579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1485723, whole genome shotgun sequence.
ACCESSION   DASUYC010000096 DASUYC010000000
VERSION     DASUYC010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5579
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485723"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VF276_01515"
     CDS             complement(<1..343)
                     /locus_tag="VF276_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605567.1"
                     /translation="MIALQRATDAIRRWRWTEFQLLIVPAVLSLVGMLIVIVVPTGNL
                     SFTWRDLWMSLLFIGLLYGTHFFLGATQRRADQVLLPVVAFITALGTIMIQRLQLSLE
                     RTYPGVGYEGVA"
     gene            complement(340..1257)
                     /gene="mtnP"
                     /locus_tag="VF276_01520"
     CDS             complement(340..1257)
                     /gene="mtnP"
                     /locus_tag="VF276_01520"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEX6605568.1"
                     /translation="MSGAEEAHAGNPAGDAPRIGIIGGSGFYNMPGLTGIEEVRVDTP
                     FGPPSDAIILGTLAGRRVAFLPRHGRGHVISPTELPVRANIWALKSLGVQRLLSISAV
                     GSMLEGIAPSDLVVPDQLIDRTVSRPRTFFGEGLVGHVALADPYCPQLRSELVASGHA
                     AGAPIHDGGTYIVIEGPQFSTRAESRLFRSWGVAVIGMTAMPEARLAREAELCYANLA
                     LATDYDSWHDVHESVTADLVMQNLARSVQAAQATVTGLIGNLVEQAAAGCTCGHALDG
                     AIMTAPDKIPATVRERLGPILERVLTTHP"
     gene            complement(1280..1648)
                     /locus_tag="VF276_01525"
     CDS             complement(1280..1648)
                     /locus_tag="VF276_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX6605569.1"
                     /translation="MRTIAVVGLSDHPGRPSYDVARYMQGQGYRIIPVNPTIREALRQ
                     PAYPSLHDVPEPVELVDIFRRSEYVADIVDEAIAIGAKGVWMQVGVIDPAAAEKARAA
                     GLLVVMDRCIKVEHSRQFSL"
     gene            2185..2733
                     /gene="pyrR"
                     /locus_tag="VF276_01530"
     CDS             2185..2733
                     /gene="pyrR"
                     /locus_tag="VF276_01530"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016148183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEX6605570.1"
                     /translation="MSGESVVILDSEQIRRVLTRMAHEILERNGGARDLLLVGVRTRG
                     VPLARRLAERMGTIERMAVPVLELDAKPFRDDLPRQAGGPAPALPALPVEGGRIVLVD
                     DVLYTGRTVRAAIDALIRAGRPAAIQLAVLVDRGHRELPIRADYAGKNVPTARTEYIS
                     VRLLETDGLDEVRLVRPAGDGA"
     gene            complement(2735..3616)
                     /locus_tag="VF276_01535"
     CDS             complement(2735..3616)
                     /locus_tag="VF276_01535"
                     /inference="COORDINATES: protein motif:HMM:NF003738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacetylase"
                     /protein_id="HEX6605571.1"
                     /translation="MTNANGELTATQAVDLERLAAFVGDADRLVVLTGAGCSTESGIP
                     DYRSPGGLWTNHKPIFYADFVRSATTRQRYWARSMAGWNSFHRAAPNPAHVALARLEA
                     LGRVQYLITQNVDRLHQAAGSRNVLELHGNNDGVVCLGCGHEMTRQAMQRALQALNPT
                     WNAHPVAVAPDGDAQLDTEAFAAFQVPGCPVCGGLLKPDVVFFGESVPPARVGQATAE
                     VAECDALLVVGSSLTVWSGYRFAHQAVTAGKPLALVNIGPTRADPIATLKIEQRVGEF
                     LPHLLTHVGPRVPAPAG"
     gene            complement(3866..5041)
                     /locus_tag="VF276_01540"
     CDS             complement(3866..5041)
                     /locus_tag="VF276_01540"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605572.1"
                     /translation="MTRQAKLSTRRRVTRLAIPLLVMIALLGALVMLGATRAAGAPLG
                     GPAAYAPLGDKGGNGGGQGNGGGQKDGKGDDQGKDKGHGQGNGNGNGNGNGNGNGQGN
                     GNGNANGHGNGNGNGQGNGNGQGNGSPGQSHGTVSKRADRATAAPGDQIQYTITLRNG
                     ATAATVVLSDKMPPHTSFVSASGPCTVTGGNNQKLDCRVTLPANGTATVQVSLRVDAN
                     APCNSMLTNVAHVNGWGSAQAQTRVICAAATATAGSGATATSTAVAATATSTAAAATA
                     TSTPPPATATGTALPATATSTAPATATATAETEATATNTPESGETATATATGTLVPPP
                     TVTPGTPTVTATPALSGSAPTLIAGSQDGTVWGIVGTVLILLGVGLAFAARSLRSTP"
     gene            5232..>5579
                     /locus_tag="VF276_01545"
     CDS             5232..>5579
                     /locus_tag="VF276_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020373340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEX6605573.1"
                     /translation="MLANIDPALRVALQRSLIGWYVATGRDLPWRRTTDPYAVLVSEI
                     LLQQTQVATVIPVYETFLARWPTFEALAAAPLAAVKEVTDPLGYHVRGEWLHRIATIV
                     TNELGGCLPDTLEG"
BASE COUNT          777 a         1871 c         1978 g          953 t
ORIGIN      
        1 tggcgacgcc ctcgtagccc acgccggggt aggtgcgctc caggctcagt tgcagccgct
       61 ggatcataat cgtgcccagg gcggtgataa aggccaccac tggcagcagc acctgatcgg
      121 cccgccgctg ggtcgcaccc aggaagaagt gcgtgccgta gagcagcccg atgaacaaca
      181 ggctcatcca gaggtcgcgc cacgtaaagc tgaggttgcc ggtcggcacg acgatcacga
      241 tcaacatgcc gaccaggctg agcacggcgg gcacgatcag caactggaac tcggtccagc
      301 gccagcggcg gattgcatcg gttgcgcgtt gtaaggcgat catgggtggg tggtcagcac
      361 acgttccaga atggggccga ggcgctcgcg cacggtggcc gggatcttgt cgggcgcggt
      421 catgatcgca ccgtccagcg cgtgcccgca tgtgcagccc gcggcggcct gctccaccag
      481 gttccctatc agcccggtga cggtggcctg cgccgcctgc acgctgcggg ccaagttctg
      541 catcaccaga tcggcagtaa cggactcgtg tacgtcgtgc cagctatcgt aatcggtcgc
      601 cagggccagg ttggcgtaac acagctcggc ttcgcgggcc agccgcgctt ccggcatcgc
      661 ggtcatgccg atcacggcca cgccccatga gcggaacaac cgcgactcgg cccgcgtgga
      721 gaactgcggc ccttcgatca ctatatacgt gccgccgtcg tggatcggtg cgcccgcggc
      781 atggccggag gcgacgagtt cgctgcgcag ttgcgggcag tatgggtcgg ccagcgcgac
      841 gtgcccgacc aggccctcgc cgaagaaggt gcgcgggcgg ctcacggtgc ggtcgatcaa
      901 ctggtcgggc accaccaggt cgctgggcgc gatgccttcg agcatgctgc ccacggcgct
      961 gatcgacagc aaccgctgga cgcccaggct tttcagcgcc cagatgttgg cccgcacggg
     1021 caactctgtg gggctgatga cgtgcccgcg cccgtgacgg ggcaggaacg cgacccgccg
     1081 cccggccagc gtgcccagga tgatcgcgtc gctcggcggg ccgaacggcg tgtccacccg
     1141 cacctcttcg atgccggtca gccccggcat gttatagaag ccggagccgc cgataatccc
     1201 gatgcgcggg gcatcccccg ccgggttgcc ggcgtgcgct tcctcggcac cgctcacgtt
     1261 gctgcgtcct ccttgcgccc tacagggaaa actgccgcga atgttcgacc ttgatgcagc
     1321 ggtccatcac cacgagcaag cccgcggcgc gcgccttctc ggcggcggcg gggtcgatca
     1381 cgccgacctg catccagacg cccttcgccc cgatggcgat ggcttcgtcg acgatatccg
     1441 ccacatactc cgagcgccgg aagatgtcga ccagttccac cggctccggc acatcgtgca
     1501 gcgagggata ggccggctgg cgcagcgcct cgcggatcgt cgggttcacc gggattatcc
     1561 ggtagccctg gccttgcata tagcgcgcta catcgtagct ggggcgccct gggtggtccg
     1621 acagcccgac gacggcgatg gtccgcatgt tgagcaagac gcggatggct tgcggatcgt
     1681 tcacgggcgg gatcgcggcg ggcaaggtgg gctccggcga cagcggggtt ccttgggtca
     1741 tggcgcttcg gttctcctat cggctctcta ccactgcaac ggttgtgcca cggatggtag
     1801 gcagcagtca gtggtcagtg gtcagtcgtg aatattgcat ggggcacggc ttcccatgcc
     1861 gggtgtatgt acattgctgc gagtatacca ttaccgggcg actcctgacc agggtcgcac
     1921 agcccgccgc gggcttcgtg gtgctagccc gcccgtcaac gggcggtcct accctgccgc
     1981 gatgcacgag acgctcgccc gccgatccgg ctcgctaccc gctcacaact gaccactgct
     2041 cgcgtagcgg tgcatagcac accaccgagc gctgaccgct gacggctgct cgcgcagcgg
     2101 tgcgtagcac atggctgaca gctacttgcg ccttgacacg gggcggcggg ctatgctacc
     2161 atgcgccgcg atgggaggcg gggcgtgagc ggcgagagtg tcgtcatcct ggacagcgag
     2221 cagatccggc gggtgctgac ccgcatggcg catgagatcc tggagcgcaa cgggggcgcc
     2281 cgcgacttgc tgctggtggg ggtgcggacg cggggcgtgc cgctggcgcg ccgcctggcc
     2341 gagcgcatgg gcaccatcga gcggatggcg gtgcccgtgc tggaactcga cgccaagccc
     2401 ttccgcgacg acctgccccg gcaggccggg gggccggcgc ccgcgttacc cgccttgccg
     2461 gtcgaggggg gccgcatcgt gctggtggac gacgtgctct acaccgggcg cacggtgcgc
     2521 gcggccatcg acgcgctgat ccgcgccggg cgccccgccg ccatccaact ggcggtgctg
     2581 gtggaccgcg gccaccgcga actgccgatc cgcgcggact acgccggcaa gaacgtgccg
     2641 acggcgcgca cggagtacat cagcgtgcgg ttgctggaga ccgacggcct ggacgaggtg
     2701 cgcctggtgc ggcccgcggg ggatggcgcc tgacctaccc ggcgggcgcc ggcacgcgcg
     2761 ggcccacgtg ggtgagcagg tggggcagga actcgcccac ccgttgctcg atcttgagcg
     2821 tggcgatggg gtcggcgcgc gtcgggccga tgttgaccag ggccagcggt ttgcccgccg
     2881 tcaccgcctg gtgcgcgaag cggtagcccg accacaccgt cagcgacgag ccgacgacca
     2941 gcagcgcgtc gcattcggcc acctccgccg tcgcctgtcc cacccgcgcg ggcggtaccg
     3001 attccccgaa gaagaccaca tcgggtttga gcaggccgcc acacaccggg cagccgggca
     3061 cctggaacgc cgcgaacgct tcggtgtcga gttgggcgtc gccgtcgggg gccaccgcga
     3121 ccgggtgcgc gttccaggtg gggttgagcg cctgcagcgc gcgctgcatg gcctggcggg
     3181 tcatttcgtg gccgcagccc aggcagacca cgccgtcgtt gttgccgtgg agttccagca
     3241 cgttgcgact gcccgccgcc tggtgcagcc ggtccacatt ctgcgtgatc aggtactgga
     3301 cgcggcccag ggcttcgagc cgggccagtg cgacatgggc cgggttgggc gcggcgcggt
     3361 ggaagctgtt ccagccggcc atcgagcgcg cccagtaccg ctgccgcgtc gtggcgctac
     3421 gcacgaagtc ggcgtaaaag atcggcttat ggttggtcca caggccgccg gggctgcggt
     3481 agtcggggat gccggattcg gtcgagcagc ccgcgccggt gagcacgacc agccgatccg
     3541 cgtcgcccac aaaggcggcc agccgttcaa ggtcgacggc ctgcgtggcg gttagttccc
     3601 cgtttgcatt ggtcatgttc cccacctgtt ctgtcgtgcg ccgctgctgg taagttcacc
     3661 gcgaaggaac gaaggacgcc aaggttgtta tgttggtttt gttcttcgcg ctctgcgcgt
     3721 cttcgcggtg cataagcgac gccggcgcgc ggatgccggt gtatcactaa ttatacggat
     3781 tgccggttct ctccgccacc gggcgaggat gcgaaacggc ctgccccgtg gggcaggccg
     3841 tcgcggatgt cgagagcgcc ggacgttacg gcgtgctgcg caggctgcgc gccgcgaagg
     3901 cgaggcccac gcccagcagg atgagcacag tgcccacgat gccccagacg gtgccgtcct
     3961 ggctccccgc gatgagggtg ggcgcgcttc cgctcagggc cggtgtggct gtgactgtcg
     4021 gcgtgcccgg cgtcacggtg ggcggcggca ccagggtacc tgtcgctgtg gcggtggccg
     4081 tctcgccgga ttccggtgtg ttggtggccg tggcttccgt ctcggcggtc gccgtggcgg
     4141 tggccggcgc ggtgctcgtc gcggtggccg ggagcgcggt accggttgcg gtagccggcg
     4201 gcggggtgct ggttgcggta gccgcggctg ccgtgctggt cgccgtggcc gcaactgcgg
     4261 tgctggtcgc cgtggcgccg ctacccgccg tggcggtggc cgccgcgcag atgacgcgcg
     4321 tctgcgcctg tgccgatccc cagccgttca catgcgcgac gttggtcagc atgctgttgc
     4381 agggggcgtt ggcgtccacc cgcaggctca cctgcaccgt cgccgtgccg ttcgcgggca
     4441 gagttacgcg gcagtccagt ttctggttgt tgccgccggt cacggtgcac ggtccggacg
     4501 cgctgacgaa gctggtgtgc gggggcattt tgtcgctcag gaccaccgtg gccgccgtcg
     4561 cgccgttgcg cagggtgatg gtgtactgga tctggtcgcc gggggcggcc gtcgcccggt
     4621 cagcgcgctt gctcaccgtt ccgtgggact gccccggcga gccgttgccc tggccgttgc
     4681 cgttgccctg gccgttacca ttaccgttgc cgtgtccatt cgcgttacca ttaccgttac
     4741 cctggccgtt gccgttgccg ttgccgttac cattaccgtt gccgttgccc tgaccatggc
     4801 ctttgtcctt gccctggtca tcgcccttgc cgtccttctg gccgccgccg ttgccctggc
     4861 cgccgccgtt cccgcccttg tcgcccagcg gggcgtaggc ggcggggccg cccagcggcg
     4921 cgcccgcggc acgggtggcg cccagcatca ccagcgcgcc caggagcgcg atcataacca
     4981 gcagcggaat cgccagacgg gtaactcgtc gtcgtgtgga aagtttcgct tgtcttgtca
     5041 agtcccaggt cctccgtatc gcgtgccccg ctggtgccag cgcccgagcg acggggccgc
     5101 gtgtatcggt ccgaaaaccc ctcctagtgt tttcaagaac ggtgcgcgtc gccgggggat
     5161 aggtttggta aggtaacaat tgcatatcaa ccgcggggtg ggccgatcag catatcgggt
     5221 ataatgcacc tatgcttgcg aatatcgacc ccgcgttgcg ggtggcgctg caacggagcc
     5281 tgattggctg gtatgtggcg accggccgcg acctgccctg gcgccgcacc accgatcctt
     5341 atgcggttct cgtctcggaa atcctgctcc agcagaccca ggtggcgacc gtgatcccgg
     5401 tctatgagac ctttctggcg cgctggccga ccttcgaggc cctggccgcc gcgccgctgg
     5461 ccgccgtcaa ggaggtaacc gacccgctgg gctatcatgt gcgcggcgag tggctgcacc
     5521 gcatcgccac catcgtgacg aatgaactgg gcggctgcct gccggatacg ctggagggg
//
LOCUS       DASUYC010000097         2462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_535250,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000097 DASUYC010000000
VERSION     DASUYC010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535250"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="VF276_01550"
     CDS             <1..724
                     /locus_tag="VF276_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605574.1"
                     /translation="VCTIGSDDWMAPDALESIAAILDRYPQVGLVHGGVTIVDAAGHA
                     HPEVAPPPYPRGLYHDVSTLFRHNYIIPPATLCRRAALLDAGPFLDHPYTQDWAMWLR
                     IGLHGWAFYGLDKAVAFYRRHGENLSDAKNFPAAHADERRMLRAVAQEYLDRLAAQGQ
                     GALHADERRLVRMLGWRSLARGDRRTARRAFAWVIRAALDVTSVPGLLLALLPSRVYA
                     EMRQVYLRFPAISRVSRRVVRH"
     gene            complement(810..2285)
                     /locus_tag="VF276_01555"
     CDS             complement(810..2285)
                     /locus_tag="VF276_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605575.1"
                     /translation="MVPERSIVDFLKPLGKHTVLIALIVLVVVAGTLIGASRSVEHTP
                     YEATAQLLLNPVSRVNLEGTGNTSSTVTDPSRIVRSAGTLMMSLDVANRVHDAAAKSD
                     NATIRALAGIEPVDLRGMITVENSQDLLVVKSHADSADVATWLANTWATEGVAAINAI
                     YAETSSSDLDKALEKARTDLAAAEKAVQDYLADNPISGLEQELKQANAFVDAATDSKT
                     KSAFALYNAGRDALQRQLDSYYAFTYSLEQTVTELKALRARIEQSPDDPNTLSNNQLS
                     LMLLQNKALAQTPAVSGTIPSQIQFQISAAPGSAPPSRDSQLRDVDSTLTALQQLQES
                     TRGQIAGLEAQLRAPIPTVAPEIVGTVAPALQQQIARINELQSELEARNFELNGLQKN
                     RDLQQNTYDLLRSRQTEQQINQQISGVANITSPASNTDTLRSRRPLNNLLLSAGMGIA
                     FALVLAVVLAYLLSLVRPNFSSNAALKRLFQRNPRRPVPAE"
BASE COUNT          352 a          763 c          867 g          480 t
ORIGIN      
        1 tgtttgcacg attggctcgg atgactggat ggcccccgac gccctggagt ccatcgccgc
       61 catcctggat cggtatccgc aagtcgggct ggtccatggg ggggtgacca tcgtcgacgc
      121 cgcgggccat gcccatccgg aggtcgcgcc gccgccctat cccaggggac tctatcacga
      181 tgtatcgacc ttgttccggc ataactacat aatcccgccg gcgactctct gccggcgggc
      241 tgcgttgctg gatgcggggc cgttcctcga tcacccgtac acgcaggact gggccatgtg
      301 gctgcgcatc gggctccacg gttgggcctt ctatggcctg gacaaggccg tggccttcta
      361 ccgccggcac ggggagaatc tctccgatgc caagaacttc ccggcggcgc acgccgatga
      421 gcggcgtatg ctgcgcgccg tggcgcagga atatctcgac cggctggcgg cccaggggca
      481 aggggcgctc cacgccgatg agcggcgact cgttcgtatg ttgggctggc ggagcctggc
      541 gcgcggcgat cgccggacgg cacgccgggc gtttgcgtgg gtgatccggg ctgctctaga
      601 tgtcacgtcg gtgccgggtc tgctgctggc gctgctaccc agccgggtct atgccgagat
      661 gcggcaggtg tacctgcgct tcccggccat cagccgcgtg agccggcgcg tggtgcgcca
      721 ctgatcgcgc ggccctctta tctcgtcgga gaataaactg cggcccccgc cctgaccggg
      781 gcgggggccg cgggctatcc gtgccccgat tactcggcgg gcacgggccg ccgcggattg
      841 cgctggaaca gccgcttgag cgcggcgttg ctgctgaagt tgggccggac gaggctgagc
      901 aagtacgcga ggaccacggc caggaccagg gcgaaggcga tgcccatgcc cgcggagagc
      961 agcaggttat tcaacggacg gcgcgagcgc agcgtgtcgg tgttgctggc cggcgaggtg
     1021 atgttggcga ccccgctgat ctgctggtta atctgctgct cggtctggcg gctgcgcagt
     1081 agatcgtagg tgttctgttg caggtcgcgg ttcttttgca ggccgttcaa ctcaaaattg
     1141 cgggcctcca gctcgctttg caactcgttg atgcgggcga tctgctgctg cagcgcgggc
     1201 gctacagtgc ccacgatctc gggcgccacg gtggggatgg gcgcccgaag ctgcgcttcc
     1261 aggccggcga tctgcccacg ggtcgattcc tggagctgtt gcagcgccgt gagtgtggaa
     1321 tcgacatcgc gcaactggct gtcgcggctg ggcggcgcgc tgccgggcgc ggcgctgatc
     1381 tggaactgga tctgcgacgg gatcgtgccg ctcaccgcgg gggtttgcgc cagcgccttg
     1441 ttctgcaaca gcatcaggct aagctggttg ttgctgagtg tgttggggtc gtccggcgat
     1501 tgctcgatgc gggcgcgcaa cgccttgagc tcggtcaccg tctgctcaag gctgtatgtg
     1561 aacgcataat agctgtcgag ttggcgctgc agggcgtccc gccccgcatt gtagagggcg
     1621 aaggcactct tcgttttgct gtcggtcgcg gcatcgacga aggcgttggc ctgtttcagc
     1681 tcctgttcga gtccgctgat cgggttgtcc gccaggtaat cctgcacggc tttctcggcg
     1741 gcggccaggt cggtgcgggc tttctccagc gctttgtcga ggtccgaact cgaagtctcg
     1801 gcatagatcg cgttgatggc ggccacgccc tccgtggccc aggtattcgc cagccaggtc
     1861 gccacgtcgg cgctatcggc gtggctcttg accacgagca ggtcctggct gttttccacc
     1921 gtgatcatcc cgcgcaagtc gacgggttct atgccggcca gggcgcggat ggtggcgttg
     1981 tcgcttttgg ccgccgcgtc atggacccgg ttcgccacat cgagcgacat catgagcgtg
     2041 ccggcggagc gcacgatgcg gctcggatcg gtcacggtgc tgctagtgtt gccggtgcct
     2101 tcgaggttga cgcggctgac cgggttgagc aggagctggg ccgtggcctc gtagggcgta
     2161 tgctcgacgg atcgggaagc gccgatcagc gtgccggcga ccaccacgag gacgatcagg
     2221 gcgatgagca ccgtgtgctt acctaacggc tttaagaaat cgacgatact gcgttccggt
     2281 accatggggc tattgcttct cctcgccagg ggacgtgatt catgcccggc acgccactgt
     2341 ccatgccccg gcactctcga cccaaaaaca cccgcattat aagcaatcat tggacggggc
     2401 gcaagcaggg aggtcaccgc gccccggcag gatgccgcga cggcgcgccg ggcgggtcgc
     2461 tg
//
LOCUS       DASUYC010000098         3137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1056015, whole genome shotgun sequence.
ACCESSION   DASUYC010000098 DASUYC010000000
VERSION     DASUYC010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056015"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2804)
                     /locus_tag="VF276_01560"
     CDS             complement(<1..2804)
                     /locus_tag="VF276_01560"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6605576.1"
                     /translation="MQCPSCNFMNVPGMRFCGQCGARLAVPLDCPVCSFLNAAGMRFC
                     GQCGSALFVGAAAAPARIDPAAATGGASVAEPAGGNGLARLVPTIDERKVVTILFGDI
                     SGFTTMAERLDPEEVKHVMDLTLRSLAAEVERYEGHVDKFLGDNIMALFGAPRAHEDD
                     PERAVRCAIEMQHAMRRLSADLERTRGLDLQLHIGINTGEVLAGRMAAGRDIDYTVMG
                     DPVNLAARLQHTAGPGEIVVGEATYRASSAVIEYRSRGHVQVRGKEQPVSAWEVVNVR
                     TRRGEERRLTGRESRLVGRDAEFAQLKHLYGQVVRDRRLHHVLLLGPAGIGKSRLLWE
                     LEKYLSGLPQAPAFRKGRAQPYGPGAGFRALGEMLKAQCGILDDDPRPVAAEKLLAGV
                     RAVVERAGGRDLPGGDPADPIGRLVRRAVAGASEAEAVAHWLGIMLGLRAVDVEPDTV
                     RTEIFWSLRCFFARQAEAAPLVLAVEDLQWADQSMLEFIEYLRETLPDHPILIVTLAR
                     PALWESGTGQAWIARSRAAPAFTQLAVGPLSEDHSRRLVRELLADADLPAAFLDLIMA
                     KTEGNPFFLEEIIRMLIDAQVIVQRDGQWKLVAAVETLHIPDTVQALVGARVDDLPEG
                     EKRLLQGASVVGRVFWAGALSEMFPQVPPAGIAESLRFLERREFIVERTGPAFAGERE
                     YSFRNLLTRDVTYISVPKALRGEEHARVAAWIEAKAGDRVDEFCDLLAYHYEKALLLG
                     LDMMTLGETATASLLDRAIHFLKTAGETAMARQALSEANGYFSRVIELLALCGREPAD
                     PAPGAAAAVHPEYLDVLCGHAEVQEGLGNYDLALLELGHVIGYAEADGAQATLARALL
                     ARATIHYTRGVLDRLEADAGAALALFSALHDPRGKAATRLLLGQMWIARDNLEAAESE
                     MTRAMQLAGQVEDRLGEARA"
     gene            complement(2938..>3137)
                     /locus_tag="VF276_01565"
     CDS             complement(2938..>3137)
                     /locus_tag="VF276_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="stage V sporulation protein R"
                     /protein_id="HEX6605577.1"
                     /translation="GDYRRSRELYLVHRHEGRDLDTVYAEKTLQYMYQIWGRPVHLET
                     IVEGDRVLLSYDGTKNTRTEL"
BASE COUNT          467 a         1106 c         1088 g          476 t
ORIGIN      
        1 atggcgcgcg cctcgcccag ccggtcctcg acctgcccgg ccagttgcat ggcgcgggtc
       61 atctcggatt cggcggcctc caggttgtcg cgggcgatcc acatctggcc gagcagcaga
      121 cgggtggcgg ccttgccgcg cgggtcgtgc aaggcgctga atagcgccag ggcggctccg
      181 gcgtcggctt ccaggcggtc gaggacgccg cgggtgtagt ggatggtggc gcgggcgagc
      241 agcgcgcggg ccagcgtagc ctgggcgccg tcggcctcgg cgtagccgat cacgtggccc
      301 aactccagca gcgccaggtc gtaattgccc aggccctcct gcacctcggc atggccgcag
      361 agcacgtcga ggtattcggg gtgaaccgcc gccgccgcgc cgggcgccgg gtccgccggt
      421 tcgcggccac agagcgccag cagttcgatc acccgcgaga agtagccgtt ggcctcgctg
      481 agcgcctggc gggccatcgc ggtctcgccc gccgtcttca ggaagtggat cgcgcggtcg
      541 agcaggctcg ccgtcgccgt ttcgccgagc gtcatcatgt cgaggccgag cagtagcgcc
      601 ttctcgtagt ggtaggccag caggtcgcag aactcgtcaa cccggtcgcc cgccttggcc
      661 tcgatccagg cggccacgcg ggcgtgttcc tcaccgcgca acgccttggg cacgctgata
      721 taggtgacat cacgggtgag caagttgcgg aagctatact cgcgctcgcc cgcgaaggcc
      781 gggccggtcc gctcgacgat gaactcgcgc cgctccagga agcgcagcga ctcggcgatg
      841 ccggcgggcg gcacctgcgg gaacatctcg ctcagcgccc cggcccagaa gacgcgcccc
      901 accaccgagg cgccctgcaa cagccgcttc tcgccttccg gcaggtcgtc cacccgcgcg
      961 cccaccaggg cctgcaccgt gtcggggata tgcagggtct cgaccgccgc caccagtttc
     1021 cactggccgt cgcgctgcac gatcacctgg gcgtcgatca gcatgcggat gatttcttcc
     1081 aggaagaagg gattgccctc ggttttcgcc atgatcaggt cgaggaaggc ggcgggcaga
     1141 tccgcgtcgg ccagcagttc gcgcaccagc cggcggctgt ggtcctcgct gagcgggccg
     1201 acggcaagct gggtgaaggc cggggccgcc cgcgagcgcg cgatccacgc ctggcccgtg
     1261 ccgctctccc acagcgcggg gcgggccagc gtgacgatca agatcgggtg gtcgggcagc
     1321 gtctcgcgca ggtactcgat gaattcgagc atgctctgat cggcccattg caggtcttcg
     1381 acagccagca ccagcggcgc cgcctcggcc tggcgggcga agaaacaacg cagcgaccag
     1441 aagatctccg tgcgcacggt gtccggctcg acatcgacgg cacgcagccc cagcatgatg
     1501 cccagccagt gggccaccgc ctcggcctcc gaggccccgg ccaccgcgcg gcgcaccagc
     1561 cggccaatgg gatcggcggg gtcgccgccc ggcagatcgc ggcccccggc tcgctcgacc
     1621 acggcgcgca cgccggccag cagtttctcg gcggcgacgg ggcgcggatc gtcgtccagg
     1681 atgccgcact gggccttgag catctcgccc agggcgcgga agcccgcgcc ggggccgtag
     1741 ggctgggcgc ggcccttgcg gaaggcgggg gcctggggca acccggagag gtacttctcc
     1801 aactcccaga gcaggcgcga cttgccgatg cccgccggcc ccagcagcaa cacgtgatgc
     1861 agccgccggt cgcgcaccac ctggccgtac aggtgcttga gttgcgcgaa ttcggcgtcg
     1921 cggcccacca gccggctctc gcgcccggtc aggcgccgct cctcgccgcg gcgcgtgcgc
     1981 acgttgacca cttcccaggc ggacaccggc tgctccttgc cgcgcacctg cacgtggccg
     2041 cgcgagcggt actcgatcac ggcgctgctg gcccggtagg tcgcctcgcc cacgacgatc
     2101 tcgcccggcc cggcggtatg ttgcaggcgg gcggccaggt tgacggggtc gcccatgacc
     2161 gtgtagtcga tgtcccggcc cgcggccatg cgccccgcca gcacttcgcc ggtgttgatg
     2221 ccgatatgca attgcaggtc aaggccgcgg gtgcgctcca ggtcggcgct gagccggcgc
     2281 atggcgtgct gcatctcgat ggcgcagcgc acggcacgct ccgggtcgtc ctcgtgggcg
     2341 cgcggcgcgc cgaagagcgc catgatgttg tcgcccagga acttgtcgac gtggccctcg
     2401 tagcgctcca cctcggcggc caggctgcgc agcgtcaggt ccatcacatg cttgacttct
     2461 tcggggtcca gccgctcggc catcgtagtg aagccggata tgtcgccgaa caggatggtg
     2521 acgactttgc gctcgtcgat ggtgggcacc aggcgggcca gcccgtttcc gcccgccggc
     2581 tcggcaacgc tcgccccgcc cgtagctgcc gccgggtcga tccgggcggg cgcggcggcg
     2641 gccccaacaa acagggcact gccacactga ccgcaaaagc gcatccccgc cgcgttgagg
     2701 aagctgcaca ccgggcaatc gagcggcacg gccagccgcg ccccgcactg cccgcagaag
     2761 cgcatccccg gcacgttcat gaagttacag gatggacatt gcatacgagt gctaacgagc
     2821 cgtcagccgt cagcggtcag gcggcctgaa ggtttgtcat gcctgacttt ggcaactggc
     2881 acgaacaaga cgtgtttata aactcacacc tgacggctga ccgctgacgg ctgatggcta
     2941 cagttccgtg cgggtgttct tggtgccgtc gtacgagagc aggacgcggt cgccctcgac
     3001 gatggtttcc agatggaccg gacgccccca gatctggtac atgtattgca gagtcttctc
     3061 ggcatacacc gtgtcgagat cgcggccttc gtggcggtgg accaggtaga gttcgcggct
     3121 gcggcggtaa tcgccgt
//
LOCUS       DASUYC010000099         4200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_340793,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000099 DASUYC010000000
VERSION     DASUYC010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340793"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..2890
                     /locus_tag="VF276_01570"
     CDS             194..2890
                     /locus_tag="VF276_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006513026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX6605578.1"
                     /translation="MLATKLYIPPSPPTAVQRARLLERLTAGLHRKLTLIAAPAGFGK
                     TTLVSAWVAGCGRPVVWLSLDAADSDPARFLTYLVTALRGVAPDIGEGVLRVLAAPQP
                     PPPEAILTSLLNEIAAVPQAFVLVLDDYHLIEVPAVDQALAFLIEHLPPQMHLVIATR
                     EDPPLPLARLRARAQLTEVRGADLRFTSAEAAAFLRAMGLNIAAEDVTALEERTEGWI
                     AGLQLAALSLRGHEDVHDFIQAFAGDHRYILDYLVEEVLQRQPAPIRRFLLQTAILDR
                     LYGPLCEAVTGQPDGSALLEDLERGNFFVVPLDDRRQWYRYHHLFGQVLSAHLRAEQP
                     DQVATLHRRASAWYEQQGAVVEAIHHALAAADFARAADLIERTVPEMRRTREVARLLS
                     WFKALPDAVLRARPVLSAEYGGVLLADGQVAGVEARLQDAERWLDPTTAMRARQDAPA
                     AMVVVDDEAFRRLPGAIAMYRAGLALVRGDVAGTMHYARQARDLAPEDDDLGRSGAVA
                     LLGLASWASGDLAAAHRTYAEGMAGVLRAGFISDAINGAIARAEIRIAQGRLHEAGRT
                     YEQALQLAAAQGEPVLQGTADLYVGLSELARERNDLPAAAEHLQRSQELGERTGFPQN
                     RSRWCVAMARIRQAQGDLAGALDLLQEAERRYVSDFFPNVRPVAAWRTRVWVAQRRLG
                     DAFDWARERGLSVEDDLSYLREFEHLTLARVLLARYTSDGTAHWVQQALGLLARLLQT
                     AEAGERTGSVIEILVLQALAHQAQGAIPAALVPLQRALALAEPEGYVRIFVDEGAPMA
                     VLLQAAARQGIAPDYSRQLLAALGPAPAGPPAKQPLIEPLSDRELEVLRLLGSDLSGP
                     EIARALMVSLNTMNTHTKNIYAKLGANNRRAAVRRAAELHLL"
     gene            3115..3858
                     /locus_tag="VF276_01575"
     CDS             3115..3858
                     /locus_tag="VF276_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018930552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605579.1"
                     /translation="MSTIRQTATSNGNTHADPTIAAHLIRWTGLAAVGGGIIFVGIQP
                     IHPPDVVASVTTSTWAIITTLKTAMCLMFLLGIGGIYARQANKAGWLGLAGWFLFSLS
                     WALQLCFVFAETFILPLLATTAPQFVDGFLRNIAGGEVTSVDLGALPVIYGIGVGGLY
                     MLGGVVFGLATLRAAVLPRWAAGLLAVAALLTPAAALFPHNIQRFAAVPVAFALAALG
                     YALWAERRARTAAPVPSPVSPQLGQTGAK"
     gene            3942..>4200
                     /locus_tag="VF276_01580"
     CDS             3942..>4200
                     /locus_tag="VF276_01580"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HEX6605580.1"
                     /translation="MNPNPHATTSGTATRKLAIAAGVCYLITHVTSVGAAILYDPILH
                     NAGYITGGGPDTPVLLGAFFEIILALAIVGTAVALFPVVKRQ"
BASE COUNT          627 a         1459 c         1425 g          689 t
ORIGIN      
        1 ctgactttgc agcgtgatgt gatggtcgcc gggatccgcc ggcgtgggac ggattggcgg
       61 gtcggtgtgg acgcgcgttg tgcagtggaa ccggctgctg ggttgccgca acggatcagc
      121 gcgtgcctct ttgcagcgct cccctccccc gttccgccgg tcgataggct ataatggatc
      181 tgtgtcgatt ccagtgttag ccacgaaact ctatatcccg ccgtcgcccc cgacggcggt
      241 gcagcgggcc cgcctgctgg agcggttgac tgccgggctg caccgcaagc tgacgctcat
      301 cgcagcccct gccggcttcg gcaagaccac gctggtcagc gcctgggtcg ctgggtgcgg
      361 gcggccggtg gtctggttgt cgctggacgc cgcggatagc gacccggccc gctttctgac
      421 ctacctcgtc acggcgttac gcggggtggc cccggatatc ggcgaggggg tgttgcgcgt
      481 gctcgcggcc ccgcagcccc cgccccccga agccatcctg acctcgctac tcaatgaaat
      541 cgccgccgtg ccccaggctt tcgtcctcgt cctcgatgac taccacctga tcgaggtccc
      601 cgcagtggac caggcgctcg ccttcctgat cgagcacctg cccccacaga tgcacctggt
      661 gatcgccacg cgggaggatc ccccgctgcc gctggcccgg ctccgcgccc gcgcccaact
      721 gaccgaggtg cgcggagccg acttgcggtt tacttccgcg gaggctgctg catttctccg
      781 ggcgatgggt ctgaacatcg cggcggaaga tgtgaccgcc ctggaagagc gcaccgaggg
      841 ctggatcgcg ggcttgcaat tagctgccct gtcgctgcgg ggccacgaag acgtgcatga
      901 cttcatccag gcgtttgcgg gcgaccatcg ctacatcctg gactacctgg tcgaagaggt
      961 gctgcaacgc caacccgccc ccatccgccg cttcttgctc cagaccgcca ttctcgaccg
     1021 cttgtatggt ccgctgtgcg aggccgtcac cggccagccg gacggcagcg cgctgctgga
     1081 ggatctggaa cgcggcaact tctttgtcgt gcccctggat gaccggcgcc agtggtatcg
     1141 ctatcaccac ctctttgggc aagtgctctc ggcgcatctg cgggctgagc agcccgacca
     1201 ggtcgcgacc ctacaccggc gcgccagcgc gtggtacgag cagcaggggg cagtggtcga
     1261 ggcgatccac catgcgctgg ccgccgccga tttcgcgcgc gcggcggacc tgatcgagcg
     1321 gactgtgccc gaaatgcgcc ggactcgaga ggtcgcccgg ctgctgagct ggttcaaagc
     1381 gctgcccgac gcggtgctcc gcgcccggcc cgtgctcagc gcggagtatg gcggggtgct
     1441 attggccgac ggtcaggtcg ccggcgtgga ggcccgcctg caggacgccg aacggtggct
     1501 ggacccgacc acggccatgc gtgcgcgaca ggacgcccca gcggcgatgg tcgtcgtgga
     1561 cgacgaggcc tttcgccggc tccctggggc gatcgccatg taccgggccg gcctggccct
     1621 ggtgcggggc gatgtggccg ggaccatgca ctacgcgcgg caggcgcgcg acctcgcgcc
     1681 ggaggacgac gatctcgggc gcagcggggc cgtggcgctg ctggggctgg cctcctgggc
     1741 gagcggggat ctcgcggcgg cgcaccggac gtatgccgag ggcatggccg gagtgctgcg
     1801 cgccgggttc atctccgacg ccatcaacgg cgccatcgcc cgcgcggaga tccggatcgc
     1861 ccaggggcgg ctccatgagg ccgggcgcac ctacgagcag gccttgcagt tggcggccgc
     1921 gcagggcgag ccggttctgc agggcaccgc agacctgtat gtgggcctga gcgagctcgc
     1981 gcgcgagcgg aacgatctgc cggcggccgc cgagcacctg cagcgcagcc aggagctagg
     2041 cgagcgcacc gggttcccgc aaaaccggtc gcgctggtgc gtggcgatgg cgcgcatccg
     2101 ccaggcgcag ggggatctgg ccggcgcgct cgatctgctc caggaagccg agcgccggta
     2161 tgtgagcgac ttcttcccca atgtgcggcc ggtcgcggcg tggcggaccc gcgtgtgggt
     2221 cgcgcagcgg cgactagggg acgccttcga ctgggcgcgg gagcgcggcc tgtcggtgga
     2281 ggacgacctc agctatctac gcgagttcga gcatctcacc ctggcccgcg tgctcctggc
     2341 ccgctatacg agcgacggga cagcccactg ggtgcagcag gcgctgggcc tgctggcgcg
     2401 cctgctgcaa accgcggaag ccggggagcg gacgggcagc gtcatcgaaa tcctggtgtt
     2461 gcaggcgctg gcccaccagg cgcaaggcgc tatcccggcc gcactcgtgc cgctgcaacg
     2521 cgcgctggcg ctggccgagc cggagggcta tgttcgcatc tttgtcgacg aaggcgcccc
     2581 catggcggtg ctgctgcaag cggccgcccg gcaggggatc gcgccggact atagccggca
     2641 actcctggcc gccctgggcc cggccccggc tggcccgccg gccaaacagc cgctgatcga
     2701 gccgctgagc gaccgggaac tggaggtgct gcgcctgctc ggcagcgact tgagcggccc
     2761 ggaaatcgcc agggcactga tggtgtcgct gaacacgatg aatacccaca ccaaaaacat
     2821 ctacgccaag ctcggagcca acaaccgccg cgccgccgtc cgccgggccg cggaactcca
     2881 cctgttataa ccaggaatcc gcacccccgg ccccggcacg ttccgtgaat cccctgccgg
     2941 ggctttttgt tttccctcgc cctgcccgac caccggatca ccagatcaat caccagatct
     3001 ggtgatgcct gctcaccatg ttggcctgta ttctcttttc aagtcaagaa cgcattcagc
     3061 gggaatgcca gagcgtgacc cacagcccga tagctcatat aaggagacaa accgatgagt
     3121 accattcgcc agaccgcaac cagtaacggg aacacacacg cggacccaac catcgccgcg
     3181 catctcatcc gctggaccgg cttagccgcg gtggggggag ggatcatttt cgtgggcatt
     3241 cagccgatcc atcccccgga cgtagtggcg tccgtcacca ccagcacctg ggcgatcatc
     3301 accaccttga agacggccat gtgcctgatg ttcctgctcg gcattggggg gatctacgcg
     3361 cggcaagcga acaaggccgg gtggctgggg ctggccggct ggttcctgtt tagcctctcc
     3421 tgggcgctcc agttgtgctt tgtcttcgcc gaaaccttta tcttgccgct gctggcgacc
     3481 acggcgccgc agtttgtgga cggcttcctg aggaacatcg ccgggggcga ggtcaccagc
     3541 gtagacctcg gcgccctccc cgtgatctat gggatcggcg tcgggggcct gtatatgctc
     3601 ggcggcgtgg tgtttggcct cgcgacgctg cgcgccgccg tcctgccgcg ctgggcggcc
     3661 gggctgctgg ccgtcgcggc cttgttgacc cccgcggcgg cgctgttccc gcataacatc
     3721 cagcggttcg cggcggtgcc ggtggcgttc gcgctggccg cgctgggcta cgcgctctgg
     3781 gcggaacggc gcgctcgcac cgcggccccc gtccccagcc cggtcagccc ccagcttggc
     3841 caaaccggag ccaagtaagc gcgccggcaa cggcatggag aagacaggcg gccggtgggc
     3901 cgcctgtctc accgggaaat cgcatgaaag gagacaaacc catgaaccct aatccgcacg
     3961 ctaccacctc tgggaccgcc acgcgcaaac tggcgatcgc ggcgggcgtg tgctacctca
     4021 tcacccacgt cacctcggtc ggcgcggcca tcctgtatga ccccattctg cacaatgccg
     4081 ggtacatcac cggaggcggc cccgacacac cggtgctctt gggagcgttc ttcgagatca
     4141 tcctggccct cgccattgtc ggcaccgccg tcgcgctgtt tcccgtcgtc aagcggcaaa
//
LOCUS       DASUYC010000100         4375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_866519,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000100 DASUYC010000000
VERSION     DASUYC010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866519"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1353
                     /locus_tag="VF276_01585"
     CDS             <1..1353
                     /locus_tag="VF276_01585"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX6605581.1"
                     /translation="RADLPVVENADSPPAGFQLRSRPGVTGEQQISITFLQATAILGR
                     VVANVKVGGTAPIVTYEVPIAGSDGEPGTGGETVTPDVLLHIDRIVQDGRDHLHFQYS
                     WPQNGELDYAEGGTVELNNVESWAQAQYANLSARARLPASEAPPGDGSGQGALTAAVR
                     ALEEIGENLYDMLFPPLLKDFYKRCAPQARTILIYSTEPWIPWEIIKPYGGGLADHDC
                     DFLCARFQVARWLTLTNTRPNPLRRQVTMRTLCPVVPPSNLPAALLESRYLTSLPARW
                     PPLALYNPLPRSADEVRAVMASGRVNLFHIATHGNFQVSDPTNAAIQIGTSQLTPASL
                     TGNPIANGFAAEQPLVFINACHSGRQGLALSGLGGWVARVVQLGASGFIGTNWEVQDD
                     LAAQFAIRFYDNLRLGSTFGAACRMARQEIRQANPGNSTWLAYVLYAHPLGTLQVPPA
                     "
     gene            1384..2529
                     /locus_tag="VF276_01590"
     CDS             1384..2529
                     /locus_tag="VF276_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605582.1"
                     /translation="MADPFAIIALINSHRADLEAALGSDGPAFFSRLDDLFDRGLAVA
                     ADMDAQGRVGDDLLALVDQYPGARAVLRAADPATFGPLTPADTPPAPPMDTAPAGHAG
                     LRAPGPEEATGGGSPPDTNAGESPAPPAAPAPADTGATPALVGVPGPATIDTPSGGGP
                     VTITLVPPPGTPAAPPGPDALANAPVAGAPGPAGPQGPPGPQGPPGPQGPAAPPASTA
                     ALTGDVTSAERLQLRKETVATVLALLVVGGTVGLALITVLFMRGSDLSTAKDVLVVMS
                     SLAGVVLGYYFGRIPSEARAEQSAQQAQQAQQVAATARSAAQSVGERALQMANEVSRI
                     REALVLQTARGTTRDIGAGAAPPADTIRGLQELQNDLENLARQARQV"
     gene            2526..3734
                     /locus_tag="VF276_01595"
     CDS             2526..3734
                     /locus_tag="VF276_01595"
                     /inference="COORDINATES: protein motif:HMM:NF022113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqB family beta-propeller domain-containing
                     protein"
                     /protein_id="HEX6605583.1"
                     /translation="MSAPLRQGRATVARLLAAGLLLGALSACDFGPSGPTTPTPIPTA
                     PSAGSALGLTPVATDTPLAATPPAPPTLNPLANAGPPATATKAPPAAPATQPPGNVPP
                     TLPVATVPPAATEAPPPAAPAPPVAGATPRWMVQSQPGDLKSPAWSPDGTHLAYVQWA
                     EQSSDIYTIAALGGPATRIATGAFNAAPAWGPAGIAFTSNRDGNFDLYLMDPSGANVR
                     RLTNNPAYEVNPTWSPDGQHLAYLGEQDGRIWIFTLALTPGAQPQRVATADQPYSNGT
                     LAWSPDGSRFAFVVLENQVTNVETVAADGGDPRRLTNYDDPQTTAGNPIWGPNGSVIY
                     VVQDEHGTVQRLVARQADGRQIAPLLPPPSLSPERFPALAPGTSTLAFISDKAGAAGL
                     YLIDLRTRLP"
     gene            3974..>4375
                     /locus_tag="VF276_01600"
     CDS             3974..>4375
                     /locus_tag="VF276_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016638307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605584.1"
                     /translation="MMNRLARAQALAVQGRREAARALYAEPYAEATGAEDPYWASVAA
                     HFLAHLAGTPEAQRDWHLRALQAADAVGHERVAAFYPSLHANLAEVYLRLHNPAQARA
                     HLAQAHAARQCLPPGTGDPSVGLLIERVTQAL"
BASE COUNT          645 a         1726 c         1432 g          572 t
ORIGIN      
        1 cgcgccgacc tgccggtggt ggagaacgcc gacagcccgc cggccggctt ccagttgcgg
       61 tcgcggcccg gcgtcaccgg cgagcagcag atcagcatca ccttcttgca ggccaccgcc
      121 atcctgggcc gcgtcgtcgc caacgtcaag gtcggcggca cggcgcccat cgtcacctac
      181 gaggtgccta tcgcgggcag cgacggcgaa ccgggcaccg gcggcgagac cgtgacaccc
      241 gatgtgctgc tgcatatcga ccgcatcgtg caggacgggc gcgaccacct gcacttccag
      301 tatagctggc cgcaaaacgg cgaactcgat tatgccgaag gcggcaccgt cgagttgaac
      361 aatgtcgaaa gctgggccca ggcgcagtat gccaacctca gcgcccgcgc ccggctgccc
      421 gcctcagagg caccgcccgg cgacggcagc ggccagggcg ctcttaccgc agcggtacgc
      481 gccctggagg aaatcggcga gaacctgtac gacatgctct ttcccccgct gctcaaggat
      541 ttctacaaac ggtgcgcgcc ccaggcgcgc accatcttga tctatagcac cgaaccctgg
      601 atcccctggg aaatcatcaa accctacggc ggcggcctgg ccgatcatga ttgcgacttc
      661 ctctgcgccc gcttccaggt ggcgcgctgg ctgacgctga ccaacacgcg gcccaatccg
      721 ctgcgccgcc aggtgacgat gcgcaccctc tgcccggtgg tgccgccctc aaacctcccg
      781 gcggccctgc tcgaaagccg ctacctgacc agcctgcccg cgcgctggcc gccgctcgcg
      841 ctctacaacc ctctgccgcg cagcgccgac gaggtgcgcg ccgtcatggc gtcgggccgg
      901 gtcaacctgt tccacatcgc cacgcacggt aatttccagg tcagcgaccc gaccaacgcc
      961 gccatccaga tcggcaccag ccagttgacg cccgcctcgc tgaccggcaa tcccatcgcc
     1021 aacggcttcg ccgccgagca gccgctggtc ttcatcaacg cctgccactc gggccgccag
     1081 gggctggcgc tcagcggcct gggcgggtgg gtggcccgcg tggtccagtt gggggccagc
     1141 ggcttcatcg gcacgaactg ggaggtgcag gacgacctgg cggcccagtt tgccatccgc
     1201 ttttatgaca acctgcgcct gggcagcact tttggcgcgg cctgccggat ggcacgccag
     1261 gagatccgcc aggccaatcc cggcaactcg acctggcttg cctatgtgct ctacgcccac
     1321 ccgctgggca ccttgcaggt gccgcccgcc tgacgcccgc ctcgccgcca aggagcttgc
     1381 cccatggccg accccttcgc cattatcgcc ctcatcaaca gccatcgcgc ggacctggag
     1441 gcggccctcg gctccgacgg tcccgccttc ttcagccgcc tggacgacct gtttgaccgc
     1501 gggctggccg tggccgccga catggacgcc cagggccggg tcggcgacga cttgctggcc
     1561 ctggtggacc agtatccggg cgcccgcgcc gtgttgcgtg ccgccgaccc ggcgacgttc
     1621 ggtccgctca cccccgccga cacgccgccc gccccgccta tggacacggc gcccgccggc
     1681 cacgccgggc tgcgcgcgcc cggtccggag gaggccacgg gcggaggaag cccgccggat
     1741 accaatgccg gcgagagtcc ggccccgcct gccgccccgg cgcccgccga tacaggggcc
     1801 acaccggccc tggttggcgt acccggcccc gccaccatcg acaccccgtc cggcggcggc
     1861 cccgtcacga tcaccctggt gcccccgccc ggcacccccg ctgcgccccc cggccccgat
     1921 gccctggcga acgcgcccgt cgccggggcg cccggccccg ccggtccgca ggggccaccc
     1981 ggtccgcagg ggccgcccgg cccgcagggg ccagcggcgc cgcctgcctc taccgccgcc
     2041 ctgaccggcg acgtaacatc ggcggaacgg ctgcaattac gtaaagagac ggtcgccacc
     2101 gtgctggccc tgctcgtggt gggaggcacg gtcggcctcg ccctgatcac agtcctcttt
     2161 atgcgcggga gcgacctgag tacggccaag gacgttctag tagtaatgag cagcctggcc
     2221 ggcgtggtcc tgggctacta cttcgggcgc atccctagcg aggcgcgcgc cgagcagtcc
     2281 gcgcagcagg cacagcaagc acaacaggtc gcggcgaccg cccggagcgc cgcgcagagc
     2341 gttggcgaaa gggcattgca gatggcgaat gaggtcagcc gcatccgcga ggcgctcgtg
     2401 ctgcaaaccg cgcgcggcac cacccgcgac atcggcgccg gagccgcgcc gccggccgat
     2461 acgatccgcg ggttgcagga actgcagaac gatctggaga acctggcccg gcaggcgcgg
     2521 caggtatgag cgccccgttg cgacaagggc gcgccacggt ggcgcgcctg ctggccgccg
     2581 ggctgttgct tggcgcgctc agcgcctgcg atttcggccc cagcggcccc accacgccga
     2641 cgcctatccc caccgcgccg tccgccgggt cggccctcgg gctcacgccg gtcgccacgg
     2701 acacgccctt ggcggccacg ccgcccgcgc cgcccacgct aaacccgctg gcgaacgccg
     2761 ggccgcctgc cacggccact aaggcgccgc ccgccgcccc ggccacgcag ccgcccggca
     2821 acgtgccgcc cacgctgccc gtggcgacgg tgccgcctgc cgctacagag gcaccgccgc
     2881 ccgccgcgcc cgcgcccccg gtggcgggcg ccaccccgcg ctggatggtc caaagccagc
     2941 ccggcgacct caagtcgccc gcgtggtcgc ccgacggcac ccacctggcc tatgtgcagt
     3001 gggccgagca aagcagcgac atctacacca ttgccgcgct gggcggcccc gccacgcgca
     3061 tcgccacggg cgcgttcaac gccgcgcccg cctgggggcc tgccgggatc gcctttacca
     3121 gcaaccgcga cggcaacttc gacctctacc tgatggaccc cagcggcgcc aacgtccgcc
     3181 gcctgaccaa caacccggcc tatgaggtca accccacctg gtcgcccgac gggcagcacc
     3241 tggcctatct gggcgagcag gatgggcgca tctggatctt cacgctggcc ctcacccccg
     3301 gcgcccagcc gcagcgcgtg gcgaccgccg accaacccta cagcaacggc accctggcct
     3361 ggtcgcccga cggcagccgc ttcgccttcg tcgtcctgga aaaccaggtc acgaacgtcg
     3421 agacggtggc cgccgacggc ggcgacccgc gccgcctgac caactacgat gacccgcaga
     3481 ccacggccgg caaccccatc tgggggccga acggctcggt catctacgtg gtgcaggacg
     3541 agcacggcac ggtgcagcgg ctggtggccc ggcaggccga cggccggcag atcgcgccgc
     3601 tgctgccccc gcccagcctc agccccgagc gcttccccgc cctggccccc ggcacgtcca
     3661 cgctcgcctt catctcggac aaagccggcg ccgcgggcct ctacctcatc gacctccgca
     3721 cccgcctgcc ctagcgagtg ctgagtgctg agggctgagt gctgagtcga gacgagtttg
     3781 gagtacggtg agggctggtg cgtgttgagt ggggagtaac gagtcgggga ggggctgtca
     3841 ctctgagcgc agcgaagagt ctcgtctcgc ccgcgcggct tgtcactctg agcgcagcga
     3901 agagtctcag cgcctctctt gtcactctga gcgcagcgaa gagtctcgtc tcgtggggga
     3961 agcagacggg ggcatgatga accggctggc gcgggcgcag gcgctggccg tgcagggccg
     4021 ccgggaggcg gcgcgggcgc tgtacgcgga accatacgcg gaggcgacgg gcgccgagga
     4081 tccctactgg gcctccgtgg cggcgcactt cctggcccac ctggcgggca cgccggaggc
     4141 ccagcgcgac tggcacctgc gcgccctcca ggcggcggac gccgtgggcc acgagcgcgt
     4201 ggcggccttc tatccctcgt tgcacgccaa cctggcagaa gtctatctgc gcctccacaa
     4261 cccggcgcag gcccgcgcgc acctcgcgca agcccacgcc gcccgccagt gcctgccccc
     4321 cggcaccggc gacccctcgg tcgggctgct catcgagcgt gtcacgcagg ccctt
//
LOCUS       DASUYC010000101         2253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_765662,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000101 DASUYC010000000
VERSION     DASUYC010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765662"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..1247)
                     /gene="plsX"
                     /locus_tag="VF276_01605"
     CDS             complement(213..1247)
                     /gene="plsX"
                     /locus_tag="VF276_01605"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEX6605585.1"
                     /translation="MKIILDAMGGDNAPLETVAGAVLAAREFDVEVVLVGREAAIKAE
                     LAKHDTAGLRLPIVDAPDVIPMDEHQPSTVWKRMKNSSLVVALNLLRDGGGDGLVSAG
                     NTGAVVASALFILKRVPGIERPCLATIFPTRSAKRPVLIADAGATPDAKPHYLLQWAR
                     MGSVYMQNVFGLQAPRVGLLSNGEEEVKGNALVQESFPLLQGAGLNFIGNVEGKDIPT
                     GSVDVVVTDGFTGNVVIKLAEGLGSMILGLLREELTANLFSKLLAAGLQPAFKRVRKR
                     MDYAEYGGAPLFGVQGLVIKAHGRSNARAIRSAIRVANQAAERGTLAAFQQIGAAPAP
                     AAATPSEVAG"
     gene            1520..>2253
                     /locus_tag="VF276_01610"
     CDS             1520..>2253
                     /locus_tag="VF276_01610"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEX6605586.1"
                     /translation="MTQEPEAGQDAGILARAQGCLLGQLAGDALGSMVEFQSAATIRN
                     THPGGLREIGPSPVFGTLAGQPTDDSELALALARTLVAHGFDIEQIAAAYGDWHESGP
                     FDEGGTTRQALSGIAAARRRGDSAATAARESASQNSQANGALMRQSPLGIWGYALDPA
                     VLDGYVRADTQLTHPNHVCQDASAAFIVALAAVIREGLDGPAAYARACAWDKAHGHSP
                     KVTQALAAAQTGRPNYEQNIGHVPIAL"
BASE COUNT          382 a          721 c          795 g          355 t
ORIGIN      
        1 gcagctattc gccctctccc gcggatgatc ggctctgtgc cgcaagttac gggccatgat
       61 tcgggaaatg ggcggggttc agcaggaggc gccaggccgg tttgaaaccg gcccctacac
      121 agcaacgatg gcatgaacaa gcaaaacaga cccgaccagg attgcttctg cccggttggg
      181 tctgtctgtg aatgattgct ggattgtcgc gcttacccgg cgacctcgct cggcgtggcg
      241 gcggcaggtg cgggggcggc gccgatttgc tggaaggcgg ccagggtgcc gcgctcggcg
      301 gcctggttgg cgacgcggat ggcgctgcgg atggcgcggg cgttggagcg gccgtgcgcc
      361 ttgatcacca ggccctggac gccgaagagg ggcgcgccgc cgtactcggc gtagtccata
      421 cgcttgcgga cgcgcttgaa ggccggttgc aagcctgcgg ccagcagttt gctgaacaga
      481 tttgcggtca gttcctcgcg caacaggccc aggatcatgc tgcccagacc ctcggccagc
      541 ttgatcacca cgttgccggt gaagccgtcg gtcacgacca cgtctaccga cccggtcggg
      601 atgtccttgc cctcgacgtt gccgatgaaa ttgaggccag caccttgcag cagcgggaag
      661 gactcctgca ccagggcgtt gcccttgacc tcttcctcgc cgttgctgag caggccgacg
      721 cgcggcgctt gcaacccgaa cacgttctgc atatagaccg accccatgcg cgcccactgc
      781 aacaggtagt gcggcttggc gtcgggcgtg gcgcccgcgt cggcgatgag cacggggcgc
      841 ttggcgctgc gggtagggaa gatggtcgcc aggcagggcc gctcgatgcc gggcacgcgc
      901 ttgaggatga agagcgccga ggcgaccacc gcgccggtgt tgcccgccga gaccaggccg
      961 tcgccgccgc cgtcgcgcag caggttgagg gccacgacca gcgacgagtt cttcatgcgc
     1021 ttccagacgg tggagggctg gtgctcgtcc atggggatca cgtcgggtgc gtccacgatg
     1081 ggcaggcgca gcccggcggt gtcgtgcttg gcaagttcgg ccttgatcgc cgcctcgcgc
     1141 cccaccagca ccacctccac gtcgaactcc cgcgccgcca gcacggcccc ggccacggtc
     1201 tccagcggcg cattgtcgcc gcccatcgca tccagtatga tcttcatcgg ctgccccttg
     1261 tgctctgttt gtggcgagta tgatgtgagc ggattatacc gcacggccgg gggcgcggca
     1321 accggcccga accggggcac gttgcgcgta tacgttcacc gcgaaggaac gaagaacgcg
     1381 aagacgattt gtattagtgg tgaagaatac atctgacagc cgcctttaga ggatggctat
     1441 tgacaggtgc actctgatag catacaaccc acaccattgt tgcagtacat cgaaccccaa
     1501 agtagggaag gagacaggga tgacgcagga gccggaggcc gggcaggacg cggggatatt
     1561 ggcgcgggcg cagggttgcc tgctggggca gttggccggg gatgcgctgg gcagcatggt
     1621 cgagttccaa tcggcggcga cgatccgcaa cacgcatccg ggcggcttgc gcgagatcgg
     1681 gccgagcccg gtgttcggca cgctggccgg gcagccgacc gacgactcgg aattggcgct
     1741 ggccctggcg cgcacgctgg tggcgcatgg cttcgacata gagcagatcg ccgccgcata
     1801 tggcgactgg cacgagagcg ggccgtttga cgaagggggc acgacccggc aggcactgag
     1861 cgggatcgcg gcggcccggc ggcgcggcga cagcgcggcg acagcggcgc gggaaagcgc
     1921 ctcccagaat tcgcaggcca acggcgcgct gatgcggcaa agcccgctgg gcatctgggg
     1981 ctatgcgctc gaccccgccg tgctcgacgg ctatgtgcgc gccgacacgc aactcacgca
     2041 cccaaaccac gtctgccagg acgcgtcggc ggcgttcatc gtggcgctgg ccgcggtgat
     2101 tcgcgagggg ctcgacggcc ctgcggccta tgcgcgggcc tgcgcgtggg ataaggcgca
     2161 cggccacagc cccaaagtaa cccaggcgct ggccgccgcg cagacgggac gcccgaacta
     2221 tgagcagaac atcggccatg tgcctatcgc cct
//
LOCUS       DASUYC010000102         3034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1635576, whole genome shotgun sequence.
ACCESSION   DASUYC010000102 DASUYC010000000
VERSION     DASUYC010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1635576"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="VF276_01615"
     CDS             <1..428
                     /locus_tag="VF276_01615"
                     /inference="COORDINATES: protein motif:HMM:NF042701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM hydroxide adenosyltransferase"
                     /protein_id="HEX6605587.1"
                     /translation="FAPCAAHVARGVAPEKLGTAIPIDSLVRLPNLQATAGAGVVRGQ
                     VVHIDRFGNVITNIPANLLAELGAGRAVTVRVGGQNICGLATAYTDVAVGAMVALISS
                     GGSLEISVRNGDASRRLGCRAGDWVECRCPTADNAPENS"
     gene            491..574
                     /locus_tag="VF276_01620"
     tRNA            491..574
                     /locus_tag="VF276_01620"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:525..527,aa:Leu,seq:cag)
     gene            702..908
                     /gene="tatA"
                     /locus_tag="VF276_01625"
     CDS             702..908
                     /gene="tatA"
                     /locus_tag="VF276_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006890816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX6605588.1"
                     /translation="MPLGPTELILILLIVVVVFGVGKLTQVGPALGKSIRGFRDAVEG
                     EPDKPAATTTPPTTPSAEDKKTIV"
     gene            complement(905..1981)
                     /locus_tag="VF276_01630"
     CDS             complement(905..1981)
                     /locus_tag="VF276_01630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX6605589.1"
                     /translation="MIRHPVHRVLVCALLPIGDTLFTTPALHAVRVRYPQARITALTY
                     PTNAGILAASPDIDEIVLAPTRARPQSPGQILQFARELRAARYDLLLEFSSYNYGLGL
                     WGGARMHTSMRLPAFWWIRPDAGVTWRRLHAVEHYANVVRRAGIPVTDWRLRMYATRE
                     EIEARDQLLHDNGVQLQEPIIGLHPGGEGLWGRKRWGTAGFARVADQLHAQFGVKIVV
                     LGGTEDARLAATLARKSSAPVLNLAGQTTLGATAALAARCALFIGNDSSPLHIAVAAG
                     TRVVGIYGVTDPRSYRPWVPGGVEGRDYAVVQSTAPCAGSFPLIGGIPLWEWIDVLRC
                     DALAAVTPEQVVAAAAPLLAAALV"
     gene            complement(1978..2229)
                     /locus_tag="VF276_01635"
     CDS             complement(1978..2229)
                     /locus_tag="VF276_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605590.1"
                     /translation="MGKRLIIHLVGEDPIEAEVDELPKSGESYIQVSNPRRRDGRPLN
                     YVTAGARSFLFPWTRISFIEVMVSEAEHREVIEFFREDR"
     gene            complement(2260..2895)
                     /locus_tag="VF276_01640"
     CDS             complement(2260..2895)
                     /locus_tag="VF276_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX6605591.1"
                     /translation="MLPVDETGATAAPVGPAPAAAGPEESQDSTPRDGSGENTGVMPP
                     PPDEVNAAPAAAAVLANPLLGDTARPAEDDTPGTLPLPGTQERDTGRVTVGSVPTRLR
                     VLVLNTGRLTDWIDDPVIHVGRTDRSANVFPNIDLETDGGHNAGVSRRHVRISRQPDG
                     YYLEDLGSINGTFLNRRRLSPGNPTELKDGDEVRLGNIVLRVVLGGFQRAS"
BASE COUNT          495 a         1040 c         1018 g          481 t
ORIGIN      
        1 tcttcgcgcc ctgcgccgcg cacgtggccc gcggtgtggc gcccgagaaa ctgggcacgg
       61 caatccccat cgacagcctg gtccgcctgc ccaatttgca ggccaccgcc ggggccgggg
      121 tcgtgcgcgg gcaggtcgtg cacatcgacc gctttggcaa cgtcatcacc aatatcccgg
      181 cgaacctgct ggccgaactg ggcgccgggc gcgccgtcac cgtgcgcgtg ggcgggcaga
      241 atatctgcgg cctcgccacc gcctacaccg atgtcgcggt cggcgccatg gtggcgctga
      301 tcagcagcgg cgggtcgctg gagatcagcg tgcgcaacgg cgacgcctcg cgccggctgg
      361 gttgccgggc cggcgattgg gttgaatgcc gctgcccgac ggcggacaat gcacccgaaa
      421 attcttgaca acgtgggaga gattctgtat aatacctctg caagttgggc gggcacccgc
      481 ccaacgatgc gccgaagtgg cggaattggc agacgcgcta cgttcagggc gtagtgacca
      541 ttgggtcgtg tgggttcgag tcccacctcc ggcaccccga gggccaggct ctagagcctg
      601 gccctctttt tgcttgcccg atctccccag ccgcgcggcc cggtggcata aagtggtata
      661 ataggagcaa cggtagctag cgcaacagaa ggaggcacac aatgccgctt ggaccgaccg
      721 aattaatctt gatcttgttg attgtcgtgg tcgtgtttgg cgtgggcaag ctgacccagg
      781 tcggcccggc gctcggcaag agcatccggg gcttccgcga tgccgtcgag ggcgagccgg
      841 acaagcccgc cgcgaccaca accccgccca ccactccgtc cgccgaggac aagaagacga
      901 tcgtctagac cagcgcggcg gccagcaacg gggcagcggc ggccacgacc tgctccgggg
      961 taacggccgc cagcgcgtcg cagcgcagca cgtcgatcca ttcccacaac gggatgccgc
     1021 cgatcagggg gaacgagccg gcgcacgggg ccgtgctctg taccacggcg tagtcgcggc
     1081 cctcgacccc acccggcacc cagggccggt aggagcgcgg gtctgtcacg ccgtagatgc
     1141 cgacgacgcg cgttccggcg gccacggcaa tgtgcagcgg gctgctgtcg ttgccgatga
     1201 acagggcgca gcgcgcggcc agcgccgccg tggcgccgag ggtcgtctgg cccgccaggt
     1261 tcagcaccgg ggccgaactc ttccgcgcca gcgtggccgc gaggcgcgca tcctccgtgc
     1321 ctcccagcac cacgatcttc accccgaact gggcgtgcaa ctgatcggct acacgggcaa
     1381 accccgccgt gccccaccgc ttgcgccccc acaagccctc gccgcccgga tgcagcccga
     1441 tgatcggctc ctggagttgc acgccgttat cgtgcagcaa ctgatcgcgc gcttcgattt
     1501 cctcgcgcgt ggcgtacatg cgcagccgcc agtccgtcac cgggatgccc gcccgacgca
     1561 ccacgttggc gtagtgctcg acggcgtgca gccggcgcca ggtcacgccg gcatcggggc
     1621 ggatccacca gaaggccggc aggcgcatgc tggtgtgcat gcgggcgccg ccccacaagc
     1681 ccaggccgta attgtaactg gaaaactcca gcagcaggtc gtagcgggcc gcgcgcaact
     1741 cgcgcgcgaa ctgcaatatc tggcccggac tctgcgggcg cgcccgcgtg ggcgcgagca
     1801 cgatctcgtc gatatcggga ctggccgcca ggatcccggc gttggtgggg taggtcaggg
     1861 ctgtaatgcg cgcctgcggg tagcgcaccc gcacggcgtg cagcgccggg gtggtaaaca
     1921 gagtatcgcc gataggcaga agcgcgcaga ccagaacgcg gtggaccggg tggcgaatca
     1981 ccgatcctcg cggaagaact cgatcacttc ccggtgttcg gcttcgctga ccatgacctc
     2041 gataaagctg atccgcgtcc agggaaacag gaacgaacgc gcgccggcag tcacataatt
     2101 caggggccgg ccatcgcgcc ggcgcggatt cgatacctgg atatagcttt ccccggactt
     2161 gggtaactcg tccacctcgg cttcaatcgg atcctcgccg acaaggtgga tgatcagccg
     2221 ctttcccatc ggtccccttc tcctctagcc acgccccgct taactggcgc gctggaatcc
     2281 acccagcacc acgcgcagca caatattgcc caggcgaacc tcgtcgccgt ctttgagttc
     2341 ggtgggattc cccggactga ggcgccggcg gttcaagaag gtgccgttaa tgctgcccag
     2401 atcttcgagg taatagccat cgggctgccg gctgatgcgc acatggcggc gcgacacccc
     2461 ggcattatgg ccgccgtccg tctccaggtc gatattgggg aagacgttgg ccgagcggtc
     2521 ggtgcgcccg acgtggatca ccggatcgtc gatccagtcg gtgagccggc ccgtgttcag
     2581 caccagcacg cgcaggcgcg tgggcaccga cccgacggtc acgcgcccgg tgtcgcgttc
     2641 ctgcgtgccc ggcagcggca gagtgccggg ggtgtcgtcc tcggcgggcc gggccgtatc
     2701 gcccagcaac ggattggcca gcacggcagc agcggcgggc gccgcgttca cttcgtcggg
     2761 cgggggcggc atcacgccgg tattctcgcc actcccgtcc cgcggggtgg agtcctggct
     2821 ctcctccggt ccggctgcgg cgggcgcggg accgaccggc gccgccgtgg cgcccgtttc
     2881 atcgacagga agcactccga tgagagagct tccgcactgc tcgcaaaaca gcgtaccctg
     2941 cacgtgcgct gcgcggcaat tggggcaaat gatcatacgt gtccttctcc gctcttagcc
     3001 tggggccggt tcgggcatgc gcatgtgctg cgtc
//
LOCUS       DASUYC010000103         2134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1485980, whole genome shotgun sequence.
ACCESSION   DASUYC010000103 DASUYC010000000
VERSION     DASUYC010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485980"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1302
                     /locus_tag="VF276_01645"
     CDS             <1..1302
                     /locus_tag="VF276_01645"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEX6605592.1"
                     /translation="AGATAPAAGSAAPAAQGSCRTFPETGKTVSQPFLDYWQTHGGLA
                     QQGFPIEETGMEQSPTDGQDYRVQYFERAVFELHPENKPPYNVLLSLLGRFRYDAKYP
                     GGAPNQQPNTDPGSRLFPETGKRVGGKFLAYWESHGGLAQQGFPISDEFTEVSELDGK
                     PYTVQYFERAVFELHPENAPPYDVLLSQLGTFRFHNLYPNGWPQALLVANDCTAPPPT
                     HDSDGDGIPDNADKCPQQPETANGVFDSDGCPDTVQDLISFARDDINNFWQQTFTASD
                     LDYIPPTQFKAYTQRISTPCGRAMLDNAFYCERDHAIYYDLNFLTRQLQTDGDFAPVT
                     IIAHEWGHLVQADLGLLDSSRYTIDIELQADCFAGAYAKHAGEEGLLEEGDLDEGVNN
                     LYKAGDESDLPWFAAGAHGQPDQRVGAFQLGLDNGADKCLQ"
     gene            1611..>2134
                     /locus_tag="VF276_01650"
     CDS             1611..>2134
                     /locus_tag="VF276_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605593.1"
                     /translation="MQLSMVVRILGLATLLIGMLPAGSAAAPPAGTWTGAAPLISITG
                     PTTAFPLPSGQVLVAGTANTSQGQVSTLERYDPATGRWTAGGPWINDRSGYVTAVLSD
                     GRVLAAGGLDKDGHFTGAAALYNPAANAWTSIPPLHTPRWGGTATLLPNGQVLVLGGV
                     GDNGKGANALATAER"
BASE COUNT          387 a          773 c          665 g          309 t
ORIGIN      
        1 gccggcgcca cggcgcccgc cgcgggcagc gctgcgcccg cggcgcaggg cagttgccgg
       61 actttccccg agacgggcaa aaccgtcagc caaccgttcc tcgactactg gcagacgcac
      121 ggcgggctgg cccagcaggg cttccccatc gaggaaacgg gcatggaaca gtcgcccacc
      181 gacggccagg actatcgggt gcagtatttc gagcgcgccg tgttcgagtt gcaccccgag
      241 aacaagccgc cctataatgt gctgctctcg ctcctgggcc gcttccgcta cgacgccaag
      301 tatccgggtg gcgcgcccaa ccagcagccg aatacggacc ccggctcgcg cctctttccg
      361 gagacgggca agcgcgtggg cggcaagttc ctcgcctact gggagagcca cggcgggctg
      421 gcccagcagg gcttcccgat ctccgatgag ttcacggagg tgtcggaact cgacggcaaa
      481 ccgtacacgg tgcagtattt tgagcgcgcc gtgttcgagt tgcaccccga gaacgcgccg
      541 ccctacgacg tgctgctgag ccagctcggc accttccgct tccacaacct gtatcccaac
      601 ggctggccgc aagcgctgct cgtcgccaac gactgcaccg ccccgccgcc cacgcacgac
      661 agcgacggcg acggcatccc cgacaacgcc gacaagtgcc cgcagcagcc ggagacggcc
      721 aacggcgtgt tcgacagcga cggctgcccc gataccgtgc aggacctgat cagcttcgcg
      781 cgggacgaca tcaacaactt ctggcagcag accttcaccg catccgacct ggactatatc
      841 ccgccgaccc aattcaaggc ctatacccag cgcatctcca cgccgtgcgg gcgggccatg
      901 ctggataacg ccttctattg cgaacgcgac cacgcgatct actatgacct gaacttcctc
      961 acccggcaac tccagacgga cggcgacttc gcccccgtga cgatcatcgc ccacgagtgg
     1021 ggccacctcg tgcaggcgga tctggggctc ctcgacagtt cccgctacac catcgacatc
     1081 gagctccagg ccgactgttt cgcgggcgcc tacgccaagc acgcgggcga ggagggcttg
     1141 ctggaagaag gcgacctcga tgagggcgta aacaacctct acaaagccgg cgacgagagc
     1201 gacctgccct ggttcgccgc aggcgcccac ggccaacccg accagcgggt cggggccttc
     1261 caactcggcc tggacaacgg cgccgataag tgcttgcagt aggcacgcgc catcgcttcg
     1321 cgccctgacg ggaagccgat ccaaccgaac atcacgaggg ccgccggtga atgataccgg
     1381 cggccctttg cttttaacaa agcgttcagg tttgatgccc tccaccggcc cgcatcctga
     1441 gtcgcgggcc ggtgttgcac ctgtaacctg ccggttacaa gccgtcaccc gcgccgtcac
     1501 gccccggtgg tacattaaat agtggtggaa tcgatcttca agcgcgctgc cccggcccgc
     1561 gctatgaacc gggcacgcac gcaacgtcga ggaagcgagg gagattcgct atgcagctgt
     1621 cgatggtagt acgcatcctc ggcctggcaa cactgctgat cgggatgcta ccggccggca
     1681 gtgccgccgc gccgcccgca ggcacctgga ccggcgcggc cccgctgatc agtatcaccg
     1741 gccccacgac ggccttcccc ctgcccagcg ggcaggtgct ggtcgcgggc acagccaaca
     1801 caagccaggg ccaggtgagt acgctcgaac gatacgaccc ggcgaccggc cggtggaccg
     1861 ccgggggccc ctggatcaac gaccgcagcg gctacgtgac cgccgtcctg agcgacgggc
     1921 gggtgctggc ggcgggcggg ctggacaagg acggccactt taccggcgcg gcggcgcttt
     1981 ataaccccgc cgcgaatgcc tggacatcca tcccgccgct ccacacgccg cgctggggcg
     2041 gcactgccac gctgcttccc aatggtcagg tgctcgtcct cggcggggtc ggcgacaacg
     2101 gtaagggcgc gaacgcgctc gccaccgccg agcg
//
LOCUS       DASUYC010000104         3011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_753377,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000104 DASUYC010000000
VERSION     DASUYC010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753377"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2453
                     /locus_tag="VF276_01655"
     CDS             <1..2453
                     /locus_tag="VF276_01655"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX6605594.1"
                     /translation="RDRLATAWGADEEATGGGYLTVWADRATYDAMLAQGLRVEIDQE
                     TTRQANTPDSSGLNSPTTFYGGYKTVEEMETFLDQKVAAYPTLAAKVDVGNSWCKDHP
                     GTCTRPAAFNGYDLLALHITNQAIPGPKPVFWYDAGIHAREIATPEVAMRFINWLLDN
                     YNTDPDAHWLVDYHDIWVMPMLNPDGHHIVEAGGGGSSPYYQRKNADNDDGCTTWPPS
                     SSNHFGTDLNRNFPFMWACCGGSSGAACGETYRGPSAGSEVEVQYVMNKIRELIPDQR
                     GPLLTDAAPITTTGVIQNMHSYAGLNLYPWGHTTTYAPNRADLHNIGAHMSALNAGGN
                     GYDYGQPPEILYAVDGDSLGWAYGELGAAAYTTELNGNGFFPAYSTVDARWNENKGML
                     TYLAKIARTPYLTTRGPDTNGPVISGGGAPQGIPAALSATINYAWTNNTYQQNVAAAE
                     YYIDAPPWAGGTPVAMTATDGAFNGSTEAVQAAIDTTGLTVGRHIVFVRGRGVNSYSG
                     FPSWGPVSAVFLDVSPSEGTATPTATHTATPPPTATAVATHTPTATVSPTATVPPTAT
                     VPPTATVPPTATVPPGATHTPTAAPSATAAPPGATATPTVTETPCAITFTDVSTEYFA
                     EPVRYLACHGIISGYSNGDGTLSYRPYNATTRGQMVKIMVNAFGVPLVTPAAPTFLDV
                     PPAHPFYTYIEAAAAAGIVSGYTCGGPGEPCPGAYFRPNNDVTRGQLSKIVVNTAGWA
                     TINPPVAHFRDVPVGSVFYPFVETAVCRGLVSGYDCGPGCYEFRPANNATRGQIAKIA
                     YNAITGAQACVPPAAVTR"
     gene            2593..>3011
                     /locus_tag="VF276_01660"
     CDS             2593..>3011
                     /locus_tag="VF276_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019009048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX6605595.1"
                     /translation="MDHASSTTDIPRRPLGVFDSGVGGLSVVAELRRLLPHEAIVYLA
                     DTANCPYGPRPAAEIRALAEQATAFLLERGAKMIVVACNTASAAGLAPLRARHGPGLP
                     IVGLVPAVKPAVALTRSGTIGVLATPATLRGALLEEV"
BASE COUNT          498 a         1185 c          930 g          398 t
ORIGIN      
        1 agcgcgaccg gctggcgacc gcgtggggcg ccgacgagga ggcgaccggc ggcggctatc
       61 tgaccgtctg ggccgaccgc gccacctacg acgccatgct ggcccagggc ctgcgcgtgg
      121 agatcgacca ggagacgacc cgccaggcca acacgcccga ctcctccggc ctgaacagcc
      181 ccaccacctt ctacggcggc tacaagacgg tggaggagat ggagaccttc ctcgatcaaa
      241 aggtggccgc gtaccccacc ctggccgcca aggtcgacgt gggcaactcc tggtgcaagg
      301 accatcccgg cacctgcacc cggcccgcgg ccttcaacgg ctacgacctg ctggccttgc
      361 acatcaccaa ccaggccatc cccggcccca agccggtgtt ctggtatgat gccggcatcc
      421 acgcgcgcga aatcgcgacg cccgaagtcg ccatgcgctt catcaactgg ctgctcgaca
      481 actacaacac cgaccccgac gcccactggc tggtggacta ccacgacatc tgggtcatgc
      541 ccatgctcaa ccccgacggc caccatatcg tcgaggcggg cggcgggggc agcagcccct
      601 actatcagcg caaaaacgcg gataacgacg acggctgcac cacctggccg cccagcagca
      661 gcaaccactt cggcaccgac cttaaccgca acttcccctt catgtgggcc tgctgtgggg
      721 gatccagcgg ggctgcctgc ggcgaaacct atcgcggccc gtccgccggg tcggaagtcg
      781 aggtgcagta tgtgatgaac aagatccgcg agctcatccc cgaccagcgc ggcccgctgc
      841 tgaccgacgc cgcgcccatc accaccaccg gcgtgatcca gaacatgcac tcgtatgccg
      901 gcctcaacct ctacccctgg ggccatacga cgacctatgc gcccaatcgc gccgatctgc
      961 acaatatcgg ggcgcacatg agcgccctga acgcgggcgg caacggctac gactacggcc
     1021 agccgcccga aatcctctac gccgtcgacg gcgactcgct gggctgggcc tacggcgaac
     1081 tgggcgccgc cgcgtatacc accgaactca acggcaacgg cttcttcccg gcctattcca
     1141 ccgtcgatgc ccgctggaac gagaacaagg gcatgctcac ctacctggcg aagatcgcgc
     1201 gcacgcccta cctgaccacg cgcgggcccg acaccaacgg cccggtcatc agcggcggcg
     1261 gcgcgccgca gggcatcccc gccgccctga gcgcgacgat caactacgcc tggaccaata
     1321 acacctacca gcagaacgtg gccgccgccg aatactacat cgacgcgccg ccctgggctg
     1381 ggggcacacc cgtcgccatg acggcgaccg acggcgcctt taacggctcc accgaggcgg
     1441 tccaagccgc cattgacacc acagggctga ccgtgggccg ccacatcgtc ttcgtgcgcg
     1501 ggcgcggcgt gaatagctac tccggcttcc caagctgggg cccggtctcc gccgtcttcc
     1561 tggacgtgag tccgtccgag ggcacggcca cgccgacggc tacgcacacc gccacgcccc
     1621 cgcccaccgc gacggcggtt gccacgcata cgcccacggc cacggtctcg cccacggcta
     1681 cggtcccgcc cacggccacg gtcccgccca cggccacggt cccgcccacg gctacggtcc
     1741 cgccgggcgc cacgcataca cccacggctg cccccagcgc cactgccgcg ccgccgggtg
     1801 ccaccgccac gccgacggtc acggagacgc cctgcgccat caccttcacc gacgtgagca
     1861 cggagtactt tgccgagccg gtgcgctacc tggcctgcca cggcatcatc tcgggctaca
     1921 gtaacggcga cggcacgctg agctaccgcc cctataacgc gacgacccgc ggccagatgg
     1981 tgaagatcat ggtcaacgcc tttggcgtgc ccctggtcac acctgccgcg cccacgttcc
     2041 tggatgtgcc gcccgcccac ccattctaca cctacatcga ggcggcggcc gccgcgggca
     2101 tcgtcagcgg ctacacctgc ggcgggccgg gcgagccgtg tccgggcgcc tacttccgcc
     2161 ccaacaacga cgtgacgcgc ggccagctct cgaaaatcgt cgtcaacacg gcgggctggg
     2221 cgacgatcaa cccgcccgtg gcccacttcc gcgacgtgcc cgttgggagc gtgttctatc
     2281 ccttcgtgga gacggcggtg tgccgcgggc tcgtctcggg ctacgactgc ggccccggct
     2341 gctacgagtt ccgcccggcg aacaacgcca cgcgcggcca gatcgccaag atcgcctata
     2401 acgccatcac cggcgcgcag gcctgcgtcc cgccggcggc tgtaacgcgc taaccggcgc
     2461 acaacacacg atcccgcggg ggtgggccac ggcggtgcgc ccgcccccgc cgcttgcccg
     2521 atcttgacaa ggcgggccgg agcggggcat actggcctgg ggcgtgcggc gcgttgcccc
     2581 aggaggcgca ggttggacca cgcgagcagc accaccgaca tccctcgccg cccgctgggc
     2641 gtcttcgact cgggcgtggg cgggctgtcg gtggtggcgg aactgcgccg cctgctgccg
     2701 cacgaagcga tcgtctacct ggccgacacg gccaactgcc cctatggccc gcggcccgcc
     2761 gccgagatcc gcgcgctggc cgaacaggcc acggcgttcc tgctggagcg cggcgccaag
     2821 atgattgtcg tggcctgcaa caccgcctcc gcggcgggcc tggcgcccct gcgcgcccgg
     2881 cacggccccg gtctgcccat cgtcggcctg gtgcccgccg ttaagccggc ggtcgccctc
     2941 acccgctccg gcaccatcgg cgtgctggcg accccggcca cgttgcgcgg cgccctgctg
     3001 gaggaagtca t
//
LOCUS       DASUYC010000105         2472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1365529, whole genome shotgun sequence.
ACCESSION   DASUYC010000105 DASUYC010000000
VERSION     DASUYC010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1365529"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VF276_01665"
     CDS             complement(<1..204)
                     /locus_tag="VF276_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605596.1"
                     /translation="MALRLPLRVRTAARPVAWVHRGVTVGALAGLTGAVGEVLLLQPG
                     RRAAGAAVLAAAMVLAGLAWRGFR"
     gene            495..1358
                     /locus_tag="VF276_01670"
     CDS             495..1358
                     /locus_tag="VF276_01670"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX6605597.1"
                     /translation="MSEVEHVLAVQNRLGEGPRWNGEEQALYWVDIENDAFHRFDPAT
                     GEHDRCVVGLPVGALGFRAAGGLVLATRDGFAVWEPQTQALRFLADPEADRPASRFND
                     GAVDCMGRFWAGTMSADPTSSLYRLDPDGSVHQMETGLTISNGIGWSPDNRTMYLTDT
                     PRNVIYAYDFDPATGAVANRRPFVHTPDEPGGPDGLAVDSEGGVWSARWGGWKVTRYD
                     PAGAMEREIRLPVECPTSCAFGGAELDELYITSAWTGVDPAHRPAQPWAGDLFRVRVG
                     IQGLPEHKYAG"
     gene            1384..2358
                     /locus_tag="VF276_01675"
     CDS             1384..2358
                     /locus_tag="VF276_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014395730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEX6605598.1"
                     /translation="MPFYTNPVFDTDFPDPMILRAPDGCYYAYATQTVVAGRILNIQV
                     ARSPDLVHWERLGDALPVKPDWARHTQCFWGPHVLAAEGRYYLYYSASPDEGEGLCLA
                     VAVATAPGGPFVDSGAPLRCGPGFANIDPMAFDDPATGQRLLYWGSGFGPIYVQPLAP
                     DRVHFEPGTEPLPLLYPHGTRGYERLIEAAYVVARDGYYYLFYSGDNCCGDHAHYAVL
                     VARAEHATGPFEPRAVIEGTPDSVILERSARWDAPGHNGVIRDAAGDDWIVYHAIDPQ
                     QRCLDASIRGDRDVRRILLIDRLVYQDGWPTVAGGVPSAEPRPGPQTD"
BASE COUNT          383 a          872 c          842 g          375 t
ORIGIN      
        1 gcggaagccc cgccacgcca ggcccgccag gaccatagcc gccgccagca ccgcggcccc
       61 agcggcgcgc cggcccggct ggagcagcag cacctcgccc acggcgccgg tcaggcccgc
      121 cagcgcgccc accgttacgc cccggtggac ccaggccacc gggcgggcgg cagtgcggac
      181 acgcagcggc aggcgcagag ccatgggcat cgactcccct cgcggtagcc ccctcggcaa
      241 actcggagtc cagtatagcg catggcaccg ggtcgcgcca tgccccttgc ctgaccaccg
      301 ttcccaaact caccgcgaag gaacgaagga aacgaagatt atttctatta gtttcattct
      361 tcgcgttctt cgttccttcg cggtgaatga tccacgcctt gggccggatg ttaggcgttg
      421 cattcgcggg gcggctcgtt tacactgggc ttgcgccccg ccgccgggcg gatcgctgga
      481 aaggagcgtg gcctgtgagc gaagtcgaac atgtgttagc cgtgcaaaac cggctgggcg
      541 aaggcccgcg ctggaacgga gaggagcagg cgctctactg ggtggacatc gaaaacgacg
      601 cctttcaccg cttcgacccg gccacgggcg agcacgaccg ctgtgtggtc ggcctgccgg
      661 tgggcgcgct cggctttcgc gccgccgggg gcctggtcct ggcgacgcgc gacgggttcg
      721 ccgtctggga gccgcaaacg caagccctgc gcttcctggc cgatccggag gccgaccggc
      781 ccgcatcgcg cttcaacgac ggcgcggtgg attgcatggg gcgcttctgg gcgggcacca
      841 tgagcgcgga cccgaccagc agcctctacc ggctcgaccc cgacggcagc gtgcatcaga
      901 tggaaaccgg gctgaccatc tctaacggca tcggctggag ccccgacaac cgcacgatgt
      961 acctcaccga cacaccgcgc aacgttatct acgcctatga cttcgacccg gcgaccgggg
     1021 ccgtcgccaa ccggcgcccg tttgtccaca cgccggacga gccgggcggg ccggatgggc
     1081 tggccgtgga cagcgagggc ggggtgtgga gtgcgcgctg gggcggctgg aaggtgacgc
     1141 gctacgaccc ggcgggcgcc atggagcgcg agatccgcct gccggtggaa tgcccgacca
     1201 gttgcgcttt cggcggggcc gaactggacg aactctacat caccagcgcc tggaccggcg
     1261 tggacccggc gcaccggccc gcgcagccgt gggccggcga cctgtttcgc gtgcgggtgg
     1321 gcatccaggg cctgcccgaa cataaatacg ccgggtaagg cgcccggccc ggagtcatgc
     1381 cgcatgccgt tctacaccaa tcccgtcttt gacaccgatt ttcccgatcc gatgatcctg
     1441 cgcgcgcccg acggctgtta ctatgcctat gccacgcaga ctgtcgtcgc cgggcgcatc
     1501 ctgaacatcc aggtggcgcg ctcgcccgat ctcgtccact gggagcggct gggcgatgcg
     1561 ctgccggtga agcccgactg ggcgcggcac acccagtgtt tctgggggcc gcacgtgctc
     1621 gccgccgagg ggcgctacta cctgtactac tcggcctcgc ccgacgaggg cgaggggttg
     1681 tgcctggcgg tggccgtggc gacggcgccc ggcgggccgt tcgtcgacag cggcgcgccg
     1741 ctgcgctgcg gccccggctt cgccaacatc gacccgatgg cctttgacga cccggccacc
     1801 gggcagcggc tgttgtactg ggggtcgggc ttcggcccaa tctatgtgca gccgctggcg
     1861 ccagaccggg tccacttcga gcccggcacg gagccgctgc cgctgctcta cccgcacggc
     1921 acccgcggct acgagcggct gatcgaggcg gcttatgtcg tcgcgcgaga cggctactac
     1981 tacctgtttt actccggcga caactgctgc ggcgaccacg cgcactacgc ggtgctggtg
     2041 gcacgcgccg agcacgcgac cgggccgttc gagccccgcg ccgtcataga gggcacgccc
     2101 gacagcgtga tcctggagcg cagcgcccgc tgggacgcgc ccggccataa cggcgtgatc
     2161 cgcgatgccg ccggggacga ctggatcgtc taccacgcga tcgatccgca gcagcgctgc
     2221 ctggacgcca gcatccgcgg cgaccgcgac gtgcggcgca tcttgctgat cgaccgcctg
     2281 gtctaccagg acggctggcc cacggtcgcg ggcggcgtgc cctctgctga gcctcgcccc
     2341 ggcccgcaga ccgattaacc ggcgacggtg attcaccgcg aagaaacgaa ggaaacgaag
     2401 aatgtttata taaacaatct tcgcgttctt cgcgtcttcg cggtgaattt ctgacctgct
     2461 acggtgcggg gc
//
LOCUS       DASUYC010000106         4074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1423806, whole genome shotgun sequence.
ACCESSION   DASUYC010000106 DASUYC010000000
VERSION     DASUYC010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423806"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="VF276_01680"
     CDS             <1..514
                     /locus_tag="VF276_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6605599.1"
                     /translation="AVRLVADLQPDVVLLDLQMPELDGVEALRAMQATTPGVRALVFT
                     AFDTDERIIAAVQAGARGYLLKGAPRDELFSAIRVVAGGGSLLQPAVAAKLIQRIAAP
                     ASPELIPLTDREREVLRLLAQGLQNKEIAGHLHISERTVKFHVSAILDKLGAGNRTEA
                     VHLAAQHGLL"
     gene            772..2559
                     /locus_tag="VF276_01685"
     CDS             772..2559
                     /locus_tag="VF276_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017749407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEX6605600.1"
                     /translation="MTTTLGRPADAPDTETMQHRWVRAAALADLDPDGHTTVTAGGQV
                     LVLWRQDGQVYAINNRCPHMGFPLDRGTCRDGILTCHWHAARFDLRTGGTFDQFADDA
                     QVYPVEVRGDEIWVDTAPQRDTRAYFRTRLRNGLEQDIRLLLAKSAIALLDGSGDARE
                     PFTIGLEFGVRNRRMGWGQGLTMLTCLANMLPYLDGEDRPLALYQGLSAVARETAGSP
                     PRFVLDALPGDPPSVGTLKGWLRRFLAVRDTEGAERIVVSAVRAGATPAELADMLFTA
                     ATDYRYIQIGHAVDFTNKAFEALDLAGWEPDRAEAVLSSVVRGIATGTRQEESNAWRN
                     PIDLVAMLDAAFPQIAGALAEGQARRGTWAGRVALANKLLEDDPQGNITALLDALRAG
                     ATEAHLAEAVTYAAALRIARFATSNEFGDWDTTLHTFSFANAMQMGVRRAPSPEVLRG
                     VFDAAMSIYLDRFLNIPAARLPQPAGDPGDPATVLAGFLPLLDKQQQVGEAAGLVARY
                     QAAGGPDARLLAVLGKALLREDRDFHTIQTVEGAFNLYRTLRGTPEATHALIAAARYL
                     AAHAPTPRGQAQTYQIALRLHRGERVFEE"
     gene            complement(2617..>4074)
                     /locus_tag="VF276_01690"
     CDS             complement(2617..>4074)
                     /locus_tag="VF276_01690"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX6605601.1"
                     /translation="YTSEEMLGRHLSILITHEDQPRIPAYLATVRDGGAFTAQALGAR
                     KDGSVFHAEIQSSTIIYRGTLHVLAVSRDITERVQAYQLLEQRVAERTREITTLLDIA
                     RNVASTLELQPLLDLILEQLKVVVDYQNAGLYTIDEDSFISAGYRGPLKQEDVVHRRI
                     PFGMLPLHTRVVRDQAPLIIDDLHTIDPAGEYGPAAALMLQTSHDSTASWLGVPLMVK
                     DRVIGMLSISSPQPGYYTQRHADLAMAIATQAAVALENAKLYERAQEVATLEERQRLA
                     RELHDSVSQALYSIGLAANAARLLIDRDPPQAAAPLDYLIALTEAGITEMRALIFELR
                     PESLEQEGLIAALEKQAAALSARHRIAVHADLGAEPVLPLEIKEALYRIGQEAMHNVV
                     KHARAKRVEIRLTVEPDKVTLRVADDGKGFAVDGAFPGHLGLRSMRERAARLDGAIEI
                     ISAPGEGTCVRATLPVALATVKDRGRQTTPQPAGA"
BASE COUNT          610 a         1392 c         1420 g          652 t
ORIGIN      
        1 agccgtgcgc ctggtcgccg atctccagcc cgacgtggta ctgctcgacc tgcagatgcc
       61 cgaactcgac ggcgtggaag ccttgcgcgc catgcaagcg accacgccgg gcgtgcgcgc
      121 gctggtcttc acggctttcg acaccgacga gcgcatcatc gctgcggtgc aggcgggggc
      181 gcgcggctat ctgctgaagg gcgcgccccg cgacgaactg ttcagcgcca tccgcgtggt
      241 ggccgggggc ggctcgctgc tgcaaccggc ggtcgccgcc aagctgatcc agcgcatcgc
      301 cgcgccggcc tcgcccgaac tgatcccgct caccgaccgc gagcgcgaag tgctgcgcct
      361 gctggcccag gggctgcaaa acaaggagat cgccgggcac ctccacatca gcgagcgcac
      421 cgtcaagttc cacgtctcgg ccatcctcga caagctgggc gcgggcaacc gcaccgaggc
      481 cgtccacctg gccgcgcaac acggcttgct ctaaccaacc ataagttcac cgcgaagacg
      541 cgaagaacga gaagatttct tatattagtt tcgttcttcg cgtccttcgc gtcttcgcgg
      601 tgcagaatca cggtagcagg ccggatgctg gactgatcta tcgaccaggg gccggccccg
      661 tccatggagc ggtggcgtac tccgtccctg gtggtagaat ggagctatcc gggccggctg
      721 cgtggccggg gcccgtgcga cgatggcgtg cgaccatgga ggaggcgacc gatgacgacg
      781 acgcttggac ggccggcgga tgcgccggat accgagacga tgcagcaccg ctgggtacgc
      841 gcggcggcgc tggccgatct ggaccccgac ggccacacga cggtgacggc gggcgggcag
      901 gtgctggtgc tctggcgcca ggacggccag gtctacgcga tcaacaaccg ctgcccgcat
      961 atgggtttcc cgctggaccg cggcacctgc cgcgacggca tcctcacctg ccactggcac
     1021 gcggcccgct tcgacctgcg caccggcggc accttcgacc agttcgccga cgacgcccag
     1081 gtctatccgg tcgaagtgcg cggcgacgag atctgggtcg atacggcgcc gcaacgcgac
     1141 acccgcgcct atttccgcac gcggctgcgc aacgggctgg aacaggacat ccgcctgctg
     1201 ctggcgaaaa gcgcgattgc cctgctcgac ggcagcggcg acgcgcggga gccgttcacc
     1261 atcgggctgg agttcggcgt gcgcaaccgg cgcatgggct ggggccaggg cctgaccatg
     1321 ctcacctgcc tcgccaatat gctgccgtat ctcgacggcg aggaccgccc gctggcgctc
     1381 taccagggcc tgagcgcggt ggcccgcgag acggcggggt cgccgccgcg cttcgtgctc
     1441 gacgccctgc cgggcgatcc cccatccgtc ggcacgctga agggctggct gcgccggttc
     1501 ctggccgtgc gcgacaccga gggggccgag cgcatcgttg tgagcgccgt gcgcgccggg
     1561 gcgacgcccg ccgaactggc cgatatgctc ttcacggcgg cgaccgatta ccgctacatc
     1621 cagatcgggc acgccgtcga tttcaccaac aaggcgttcg aggcgctgga tctggcgggc
     1681 tgggaaccgg accgcgccga ggcggtgctg agcagcgtgg tgcgcggcat cgccaccggc
     1741 acccgccagg aggagagcaa cgcctggcgc aacccgattg acctggttgc catgctcgac
     1801 gccgccttcc cgcagatcgc cggggcattg gccgagggcc aggcgcgccg ggggacctgg
     1861 gccgggcggg tggccctggc gaacaagctg ctggaggacg atccgcaggg caacatcacc
     1921 gccctgctcg acgccctgcg cgcgggtgcg accgaggccc atctggccga ggccgtgacc
     1981 tatgccgccg cgctgcgcat cgcccgcttc gccaccagca acgagtttgg cgactgggac
     2041 accacgctgc acacgttctc cttcgccaac gccatgcaga tgggcgtgcg ccgggcaccc
     2101 tcgccggagg tgctgcgcgg ggtcttcgac gcggcgatga gcatctacct ggaccgcttc
     2161 ctcaatatcc ccgcggcgcg gctgccgcag cccgcgggcg atcccggcga tcccgccacc
     2221 gtgctggccg ggttcctgcc gttgctcgac aagcagcagc aggtgggcga ggccgcgggc
     2281 ctggtggcgc gctaccaggc ggcgggtggg ccggacgcgc gcctgctggc cgtgctgggt
     2341 aaggccttgc tgcgcgagga tcgcgacttc cacaccattc agacggtgga aggcgccttc
     2401 aacctctatc gcaccctgcg cgggacgccg gaggccacgc acgccctgat cgccgccgca
     2461 cgctacctgg cggcccatgc gcccacgccg cgcggccagg cacagacgta ccagatcgcc
     2521 ttgcgcttgc atcgcggcga acgggtcttc gaggagtagg ttctagccca ggttgggtct
     2581 gccgccccgc cgctgcagca gcggcggggc tttgcttcag gcaccggcgg gctgcggtgt
     2641 ggtctggcgt ccgcggtctt tcacggtcgc cagggcgacc ggcagcgtgg cgcgcacaca
     2701 ggtgccctcg ccgggcgcgc tgatgatttc gatcgcgccg tcgaggcggg cggcgcgttc
     2761 gcgcatcgag cgcaggccca ggtggccggg aaaggcgccg tccaccgcga agcccttgcc
     2821 gtcatcggcc acacgcagcg tgactttgtc cggttccaca gtcaggcgga tctcgacccg
     2881 cttggcgcgg gcatgtttca ccacgttgtg catggcttcc tgcccgatcc ggtacagcgc
     2941 ctctttgatt tccagcggca ggaccggctc cgcccccagg tcggcgtgga cggcgatgcg
     3001 atggcgcgcg ctcagggccg cggcctgttt ttccagcgcg gcgatcaggc cctcctgctc
     3061 cagcgactcg ggacgtagct cgaagatgag cgcgcgcatc tcggtgatgc cggcttcggt
     3121 cagggcgatg aggtagtcga gtggggcggc ggcctgcggc gggtcgcggt cgatcagcaa
     3181 ccgggcggcg ttggcggcca ggccgatact gtagagcgcc tgggagaccg agtcgtgcag
     3241 ttcgcgggcc agccgctggc gctcctccag cgtcgccacc tcctgcgcgc gctcgtacag
     3301 cttggcgttt tccagggcca cggcggcctg ggtggcaatc gccatggcga ggtcggcgtg
     3361 gcgctgggtg tagtaaccgg gttgggggga gctgatactg agcatgccga tcacgcggtc
     3421 cttgaccatg agcggcaccc ccagccagga ggccgtactg tcgtgcgacg tttgcaacat
     3481 caacgccgcc gccggcccgt actcgccggc cgggtcgata gtgtgcaaat cgtcgatgat
     3541 cagcggggcc tgatcgcgca cgaccctggt gtgcaggggc agcataccaa aggggatgcg
     3601 ccggtgcacc acatcctcct gtttgagcgg cccgcgatac cccgcgctga tgaagctgtc
     3661 ttcgtctatc gtataaaggc cggcgttctg gtaatcgacc acgaccttga gttgctccag
     3721 gatcaagtcg agcagcggtt ggagttcgag ggtcgatgcc acattgcgcg cgatgtcgag
     3781 cagggttgtg atctcgcgtg tgcgctcggc cacgcgctgc tccagcaact gataggcttg
     3841 cacccgttcc gtaatatcac gcgacacggc gaggacgtgg agcgtacccc ggtagatgat
     3901 cgtgctgctc tggatttccg cgtgaaacac ggacccgtct ttgcgtgcgc ccagggcttg
     3961 cgctgtaaag gcgccgccat cgcgcacagt cgccaggtat gccggaatcc gcggttggtc
     4021 ctcgtgggta atcaagatgc tcaggtggcg tccgagcatc tcctcggagg tata
//
LOCUS       DASUYC010000107         3669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1144270, whole genome shotgun sequence.
ACCESSION   DASUYC010000107 DASUYC010000000
VERSION     DASUYC010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1144270"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VF276_01695"
     CDS             <1..429
                     /locus_tag="VF276_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6605602.1"
                     /translation="DLPLDEEVLQLAFSLVAPLGDRRNKQRARIEIIHVIEVPQALPL
                     DAELPEAVQNGEGVLARAEQLARQREVEVSAEMLQARSPGVAIVDEAVERGADIIIMG
                     ARPRRLHGEYHMGNTLPYVFKNAPCRVLVNRAARAESELK"
     gene            520..1086
                     /locus_tag="VF276_01700"
     CDS             520..1086
                     /locus_tag="VF276_01700"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEX6605603.1"
                     /translation="MKIVIMGCGRVGARLAQLFDQEGHDVAIVDLHTESFDRLGPNFH
                     GTTMAGTGIDEDVLKSVGIEHADLFLAVTNRDNSNIMAAQIAKMTFRVPRVIARIYEP
                     DREETFHQLNLETICPTTLISNRIYEAVTTGEDAHIARVEPAPLPTLPAPIESVAAGP
                     VEEPAGVGHAGTPAREHRGHSLRQRFFR"
     gene            1127..1786
                     /locus_tag="VF276_01705"
     CDS             1127..1786
                     /locus_tag="VF276_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX6605604.1"
                     /translation="MYVIVVGGGKVGYYLTKTLVNEGYEILLIEKNPVKVNTFQDRFG
                     SVVVQGDGCEVMTLERAGAARADVVIAVTGDDEDNLVICQVAKQRFHVRKTIARVNNP
                     KNEEIFRRLGIDVTVSQTNVILSLIEQLIPDRPMVHLMTLKHAQMTIVETTVSEDSLV
                     VGSPLADIKLPPNVVLSAIMRDGELMIPNGTTILQAGDEVIAVAQRSSEAPLRRLLAG
                     S"
     gene            complement(1869..>3669)
                     /locus_tag="VF276_01710"
     CDS             complement(1869..>3669)
                     /locus_tag="VF276_01710"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX6605605.1"
                     /translation="NTLLANRYTLRDQLGAGGAAVVYRAEDLRLGRIVAVKVLRPEFV
                     GNPEQAARFENEARAAAALTAPNVVDVYDYGRDAGTLFIVMQYIDGEDLKHLIRTRGP
                     LRPQEAARIAADVCTGLEAAHERGLIHRDVKPQNILIDRHGVVRITDFGVAKALAGPG
                     LTQAGTTYGTAAYLSPEQATGGPISPATDVYALGIVLYEMLCGQPPFEGDNAAAVAYK
                     QVYEPPPPLRDCAPDVPPPLEAIVARAMAKDPAARYATAADMAGDLQGFLLTSGSALS
                     PGPPAKPAPWVPPATAGAAPAPVAVALPPAPVPDQPTATYGARPRDDGSAYATWDGAE
                     AAHRTGPGGGKLVWVAIPVLVLLLVGGFVGGRMLMNRAPGPPPSPTVGGGAAPPPATA
                     TVIAAVPPAATATARPPTATPAAITVPRNTPAPTAPPAPATPTGSKGHPVFPTPTPPT
                     AADRRVTLEDLAFAGGYRYAPPSVYEGRTAVWVYGQGTSFAAMTAPFVLPAQPTGNGH
                     LTLAGMDSEDRAKTPMRILINGQSIFNGANPLPNDFSPNAGTWGTYTWTFPAAVLRPG
                     DNTITVENLAPSAALGIPFIMIDSATLTWDGEQ"
BASE COUNT          615 a         1185 c         1307 g          562 t
ORIGIN      
        1 gacttgccgc tggacgaaga agtactacag ttagcttttt cgttggtggc gccgctgggc
       61 gatcgacgca ataaacaacg ggccaggatt gaaatcattc atgtgatcga agtgccgcag
      121 gccctgccac tggatgcaga attgccggaa gcggtccaaa acggcgaagg agtgctggcg
      181 cgagccgagc aactggcgcg tcagcgcgaa gtcgaagtca gcgccgaaat gttgcaagca
      241 cgatcaccgg gcgtggcgat cgtggacgaa gccgtggagc ggggggcgga tatcattatc
      301 atgggcgccc ggccgcgccg cctgcacggc gagtatcata tgggcaatac cctgccgtat
      361 gtgttcaaga acgcgccctg ccgggtgctg gttaatcgcg ctgcacgagc agagtcggag
      421 cttaagtaga aaacccagcg acgagcgcca ccggccggcc cggccttgcg gtggcgcttt
      481 cctgttgccg cccgtgcagc agaaggagtg tggacaggca tgaaaatcgt catcatgggc
      541 tgcgggcgag tcggcgcccg gctggcccag ctcttcgacc aggagggcca cgacgtggcg
      601 atcgtggatc tgcacaccga gtcgtttgac cggctcgggc ccaatttcca cggcaccacc
      661 atggcgggca ccgggatcga cgaggatgtg ttgaaatcgg tcggcatcga gcacgccgat
      721 ctgttcctgg ccgtgaccaa ccgcgacaac tcgaacatca tggccgcgca gatcgccaag
      781 atgaccttcc gcgtgccccg cgtcatcgcg cgcatctacg aacccgaccg ggaagagacc
      841 ttccaccagt tgaacctgga aacgatctgc ccgaccacgc tgatctccaa ccgcatttac
      901 gaagcggtga ccaccggtga ggacgcccat atcgcgcgcg tcgaaccggc accgctgccc
      961 acgctgcccg cgccgatcga gtcggtagcc gccgggccgg tggaagaacc cgccggcgtc
     1021 ggccatgccg ggacgccggc acgggagcat cggggccatt cgctgcgcca gcggttcttc
     1081 cgttagccgc agtaactgag gctgccgcgc agccgggagt gataatatgt atgtgatcgt
     1141 ggtggggggt ggcaaagtcg gctactacct caccaagacc ctggtcaacg agggttacga
     1201 gatcctgttg atcgagaaga acccggtcaa ggtcaatacc ttccaggacc gctttggctc
     1261 ggtggtcgtc cagggcgacg gctgcgaggt gatgacgctc gaacgggcgg gggcggcgcg
     1321 cgccgacgtg gtcatcgccg tcaccggcga cgacgaggat aacctggtca tctgccaggt
     1381 ggcgaagcag cgcttccatg tgcgcaagac catcgcgcgg gtcaacaacc cgaagaacga
     1441 ggagatcttc cggcgcctgg gcatcgacgt gaccgtcagc cagaccaacg tgatcctgtc
     1501 gctgatcgag cagttgatcc ccgaccggcc catggtccac ctgatgacgc tcaagcacgc
     1561 gcagatgacc atcgtggaga ccaccgtcag cgaggactcg ctggtggtcg gctcgccgct
     1621 ggccgacatc aagctgccgc ccaacgtggt cctctcggcg atcatgcgcg acggcgagtt
     1681 gatgatcccc aacggaacca cgatcctgca ggccggcgac gaggtgatcg cggtggccca
     1741 acgcagcagc gaagccccgc tgcgccgctt gctcgccggc tcataacctc gcttgattca
     1801 agcaaacacc agaaaccgcc ccggttgcga tacaccgggg cggttgcgct ttccgggcgg
     1861 cccgatagct actgctcgcc gtcccaggtg agcgtggccg agtcgatcat gatgaagggg
     1921 atgcccaggg ccgccgacgg cgccaggttc tcgacggtga tcgtattatc gcccggccgc
     1981 agcacggcgg cggggaaggt ccaggtgtag gtgccccagg tcccggcatt ggggctaaag
     2041 tcgttcggca gcgggttggc gccgttgaag atcgactggc cgttgatcag gatacgcatg
     2101 ggcgttttgg cgcggtcctc ggaatccata ccggccaggg tcaggtggcc gttgccggtg
     2161 ggctgcgcgg gcaggacgaa gggcgcggtc attgcggcga aggacgtgcc ctgcccgtag
     2221 acccagaccg ccgtgcgccc ctcatagacg ctgggcgggg catagcgata gccgccggca
     2281 aatgccagat cctccagcgt cacgcgccga tccgcggcgg tgggcggggt gggcgtagga
     2341 aacaccgggt ggcccttgct gccggtcggg gtggcgggcg ccggcggcgc cgtgggcgcg
     2401 ggcgtgttgc ggggcacggt aatggccgcg ggcgtcgccg taggggggcg cgccgtcgcg
     2461 gtcgccgccg gaggcacggc ggctatgacg gtggccgtgg cgggcggagg cgccgcgcct
     2521 ccgcccacgg tcggcgaagg cggcggaccg ggtgcgcggt tcatgagcat gcgcccgccg
     2581 acaaacccgc ccaccagcag cagcaccagt accgggatcg ccacccagac cagtttgccc
     2641 ccgcccggcc ccgtccggtg ggcggcctcg gcgccgtccc aggtagcata ggccgagccg
     2701 tcgtcccgcg ggcgggcgcc ataggtggca gtgggctggt cgggcaccgg cgcgggcggc
     2761 agggcgacgg ccaccggggc gggcgccgcg cctgcggtag cgggcggcac ccagggcgcc
     2821 ggcttggcgg gtgggcccgg cgacagggcg ctgccgctag tcagcaggaa gccctgcagg
     2881 tcgccggcca tgtcggcggc ggtggcatag cgcgcggccg ggtcttttgc catggcccgc
     2941 gcgacaatcg cttcgagtgg cggcggcaca tccggggcac aatcgcgcag ggggggcggc
     3001 ggctcgtaga cctgtttgta ggccacggcg gcggcgttgt cgccctcaaa cggcggttgc
     3061 ccgcagagca tctcataaag cacgatgccg agggcataca cgtcggtcgc cgggctgatc
     3121 gggccgccgg tggcctgctc cggcgagaga taggcggcag tgccgtaggt ggtgccggcc
     3181 tgggtcagcc ccggcccggc cagcgccttg gcgacgccga agtcggtgat gcgcaccacc
     3241 ccgtgccggt cgatcagaat attctgcggc ttcacgtccc ggtggatcag gccgcgctcg
     3301 tgggcggcct ccagcccggt gcagacatcg gcggcgatgc gtgccgcttc ttgcgggcgc
     3361 aacgggcccc gcgtgcggat caggtgcttg aggtcctcgc cgtcgatata ctgcatgacg
     3421 atgaacaggg tgccggcgtc gcggccatag tcatacacat cgacgacgtt gggcgccgtg
     3481 agcgcggcgg cggcgcgcgc ctcgttctcg aagcgcgccg cctgctcagg gttgccgaca
     3541 aactcgggtc gcagcacctt caccgcgaca atacggccca gccgcaggtc ttctgcccgg
     3601 tagaccaccg cggccccgcc cgcccccagt tggtcccgca acgtatagcg atttgccagt
     3661 aaggtgttt
//
LOCUS       DASUYC010000108         4089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1918732, whole genome shotgun sequence.
ACCESSION   DASUYC010000108 DASUYC010000000
VERSION     DASUYC010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1918732"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2338)
                     /locus_tag="VF276_01715"
     CDS             complement(<1..2338)
                     /locus_tag="VF276_01715"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6605606.1"
                     /translation="MTERPSAFGYALRRRRKALDLTQAELATRAGCVLTTIKKIETGA
                     RRPSPQLAERLAGALRLSGAERAMLLGALEDVGPSRPPALPARPLPAALPIPPGPLIG
                     RGRDVAALLSLLRDPTTRLVTLTGPGGVGKTRLALEIATDLRDAFVDGVWFVDLAPLR
                     APEAVPGAIVRALGFESDAAPLAVLTRMLHDQHALIVLDNFEQVVGAAPVVAQLLGAA
                     PYLTILVTSRAPLRLTPEQEYAVPPLELPPEQDAHALDTYAAVQLFVRRARAVEPHFA
                     LTAENGAAVAAICRRLDGLPLAIELAATRVRLLTPPALLRRLDRRLDLLTEGPRDLSP
                     RQQTLRATLDWSYHLLGARARRLFARLAVFVGGAAPDAIEVVCGPSARGDVLAGLTIL
                     VEQSLIRPVADAAGEPRFVMLETIREYARERLTAGGEETTTRARHAAYYRDLAEAAVP
                     RLRGPAQVQWLDWLEADHDNLRAACEWYLESGDPEEALRLAGALHWFWDRRGYLEEGR
                     ARIQAALAAAAHVEDAGATLRRARAWALVGAAALAFDQGDRAAVAALAEESAALFRQL
                     DDRRGLVLALVRLAFAVSAAEPQRAGGLIAEARTLARAVDDPWFVGLVGFVSAQAALF
                     GAGDTTAARAAITEAVSALQAGDPYLLAHGLGTRGLIELADGALTPAREALEQALAVI
                     RTLRDTRSLALLAATTADVARCQGDYDRAADLYSESLALYHELGNHAEIPAILHNQGY
                     VALGTHDLAAARELFTTSLRRQYLAGNQAGIAEGLAGLA"
     gene            complement(2667..3140)
                     /locus_tag="VF276_01720"
     CDS             complement(2667..3140)
                     /locus_tag="VF276_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003336594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEX6605607.1"
                     /translation="MAETKVTIEGVEIKDLVTHPDERGFFREIIRVTDSFFGEGFGQL
                     SHSLMYAGVGKAWHIHKTQIDWWYVPIGALKVALHDMRPDSPTHGVTMELFMGEAYPE
                     RVLKIPPGVAHGCRALTLSHLFYVTSNTYDPAEEGRIAHDDAEIGYDWTAFPSIK"
     gene            3304..>4089
                     /locus_tag="VF276_01725"
     CDS             3304..>4089
                     /locus_tag="VF276_01725"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX6605608.1"
                     /translation="MTAIHFSSRTKTWLVWGMVLLALGFLYCVRPIVTPFVWAFVTAY
                     ILNAAVVFAMRRVGGPRWAWATLIYFGLVAGLVWLITAVAPVLVMQIQQLANEVPNYL
                     RQFELFTQNNNLPFGMSHWSAREISAGIDRTLNEFAGGLGARAPELARDAFDVLLKLL
                     LYLIATFSMLLQADRFTRSVRRLFSTAVLTELDPWFKRINNTLGSYLRGQALLIAIVS
                     TATFIGLELLGVRFAVALAIMSGLVETIPYLGPYAAGAIAVLVA"
BASE COUNT          606 a         1401 c         1452 g          630 t
ORIGIN      
        1 ccgccagccc ggccagtccc tcggcgatgc cggcctggtt cccggccagg tactggcggc
       61 gcaggcttgt agtgaatagc tcgcgggctg cggcgaggtc gtgcgtgccg agggccacgt
      121 agccctggtt gtgcaggatg gccgggatct cggcgtgatt gcccaactcg tggtacagcg
      181 ccaggctctc gctgtacagg tccgcggccc ggtcgtaatc gccctggcag cgggcaacgt
      241 cggcggtcgt ggccgcgagg agggcgagag agcgggtatc gcgcagcgtg cggatcaccg
      301 cgagggcctg ctccagggcc tcgcgcgccg gggtcagggc gccgtcagcc aactcgatca
      361 ggccacgcgt gcccaggccg tgcgcgagca agtaggggtc gccggcctgt agggcggaca
      421 cagcttcggt aatagcggcg cgcgccgctg tcgtgtcgcc ggcaccgaag agcgcggcct
      481 gggccgacac aaacccgaca aggcccacga accaggggtc atccaccgca cgggcgaggg
      541 tgcgcgcttc ggcgatcagc ccgcccgccc gctggggctc ggcggcactg acggcgaaag
      601 ccagccgcac cagcgccagc accaacccgc gccggtcgtc cagttggcgg aagagcgcgg
      661 cgctctcctc ggccagggca gccaccgccg cccggtcacc ctgatcaaag gccagcgccg
      721 ccgccccgac cagcgcccat gcccgcgccc gccgcagggt ggcgccggcg tcctccacgt
      781 gtgccgcggc ggcgagagcc gcctgaatcc gcgcgcggcc ctcctccagg tagccgcgcc
      841 ggtcccagaa ccagtgcaac gcgccggcca ggcgcaacgc ttcctccgga tcgcccgatt
      901 ccagatacca ctcgcaggcc gcgcgcaggt tgtcgtgatc ggcctccagc cagtccagcc
      961 actgaacttg cgcgggcccg cgcaggcgcg gcacggccgc ttcggcgaga tcccggtagt
     1021 aggcggcgtg ccgggcgcgg gtggttgtct cctcgccgcc cgccgtgagc cgttcccgcg
     1081 cgtactcgcg gatggtctcc agcatcacga agcggggctc gcccgcggcg tccgccaccg
     1141 ggcggatcag gctctgttcg accaggatcg tcagaccggc gagcacatcg ccgcgcgcgg
     1201 acgggccgca caccacctcg atggcgtcgg gcgcggcgcc tcccacgaac acggccagcc
     1261 gggcaaagag ccgccgcgcg cgggcgccga gcaggtggta gctccagtcg agtgtggcgc
     1321 gcagcgtctg ctggcgcggg gagaggtcgc gcggcccctc ggtgagcagg tccaggcgtc
     1381 ggtcgagccg ccggagcagg gcgggcggcg tgagcagccg gacccgcgtg gcggcgagct
     1441 cgattgccag gggcaagccg tcgagccgcc ggcagatcgc ggccaccgcc gccccattct
     1501 cggcggtcaa cgcgaagtgc ggctcgaccg cgcgggcgcg ccggacgaag agttgcaccg
     1561 ccgcgtaggt gtcgagcgcg tgtgcgtcct gctccggcgg cagctccagg ggcggcacgg
     1621 cgtactcctg ctcaggcgtc aggcgcagcg gggcgcggct ggtgaccagg atggtgagat
     1681 agggcgcggc gcccaggagc tgcgcgacga ccggcgccgc gccaaccacc tgctcgaagt
     1741 tatcgagcac gatcagcgca tgctggtcgt gcagcatgcg cgtcaggacg gcgagcgggg
     1801 ccgcgtccga ctcgaacccg agggcacgga caatggcccc cggcacggcc tccggggcgc
     1861 ggagaggcgc gagatcgacg aaccagacgc cgtcgacaaa ggcatcccgc aggtcggtgg
     1921 cgatctccag cgccagccgg gtcttgccga ctccaccggg gccggtgagg gtgaccaggc
     1981 gcgtggtggg gtcgcgcagg agggatagca gcgcggccac gtcccgtccg cgcccgatga
     2041 gcgggccggg cgggatcggg agcgccgcag gtagcggccg cgcggggagc gcagggggtc
     2101 tgctggggcc gacatcttcc agcgcaccca gtagcatggc gcgctccgcg ccggacagcc
     2161 ggagcgcgcc ggccaggcgc tcggcgagtt ggggcgatgg tctgcgggca ccggtctcga
     2221 tcttcttgat cgtagtcagc acgcagccgg cgcgagtggc gagttcggct tgcgtcagat
     2281 ccagggcttt gcgccgccgg cgcagtgcgt agccgaacgc cgatgggcgc tccgtcatgg
     2341 cgcggacccg acccgaacgg caagctcatg catattgcag atccttcgcg caagtagacc
     2401 gcggccggat gggcaacgtg cggctcgtgc cggggcaacc tgcgctgcct accctggctg
     2461 ctctgggctc attgtaccag atcgggtgcc ggccgggctc ccccataggc gcgccccgcc
     2521 cggactgtac accaaaggta gccgcaagca accagcgctg ccggcgtccg cggaatccgg
     2581 cccaggcgat agcttgccgg cccgccccgt cgccaaaaaa agcccctccc cgccgcgggg
     2641 aggggttggg gagaggttcc ccgcgcctac ttgatgctcg ggaacgccgt ccagtcgtag
     2701 ccgatttcgg cgtcgtcatg ggcgatgcgg ccctcttcgg cggggtcgta ggtgttcgag
     2761 gtgacgtaaa acaggtggct gagcgtcagg gcgcggcagc cgtgggctac gccgggcggg
     2821 atcttgagca cgcgctccgg gtacgcctcg cccatgaata attccatggt cacgccgtgg
     2881 gttggcgagt cggggcgcat atcgtggagc gcgaccttga gcgcgccgat gggcacgtac
     2941 caccagtcga tctgcgtctt gtggatgtgc caggctttgc ccaccccggc gtacatcagg
     3001 gaatggctca gttggccaaa gccctcaccg aagaagctgt cggtgacgcg gatgatctcg
     3061 cggaagaacc cgcgctcgtc cgggtgcgtg acgaggtcct tgatttccac gccttcaata
     3121 gtgactttgg tttcggccat gcgccgtgaa tcctcctgtt gcctgcggtc cgataaatcg
     3181 cctcattgta ccatgccgcc cgcgacagcg tcaaacttta cgagcggtgg gggacagatg
     3241 tccctccgga gataggaaga gtgggaattg tgtcgcggga cccgctatgt tatgattaag
     3301 ggtatgactg ctattcattt tagttcgcgt accaagacgt ggctggtctg gggcatggtg
     3361 ctgctggccc tgggcttcct ttattgcgtg cggcccatcg tcaccccgtt cgtctgggcc
     3421 tttgtcaccg catatatcct caacgcggca gtcgtattcg cgatgcgccg ggtgggcggc
     3481 ccgcgctggg cctgggccac cctgatctat ttcggcctgg tcgccgggct ggtctggctc
     3541 atcaccgccg tggcgccggt gctcgtcatg cagatccagc agttggcgaa cgaagtcccc
     3601 aactacctgc gccagttcga gctattcacc cagaacaaca atctcccctt cggcatgtcg
     3661 cactggtcgg cgcgcgagat cagcgcgggc atcgaccgga ccctgaacga attcgccggg
     3721 ggcctgggcg cgcgggcgcc ggaactggcc cgcgatgcct tcgacgtgct cttgaagctg
     3781 ctgctctacc tgatcgccac cttctcgatg ctgctacagg ccgaccgctt cacccgcagc
     3841 gtgcgccgcc tgttctccac cgcggtgctg accgaactcg atccctggtt caagcgcatc
     3901 aacaacacgc tcggctcgta cctgcgcggc caggcactgc tgattgccat cgtcagcacg
     3961 gccacgttca tcggcctgga gttgctgggg gtgcgcttcg ctgtggctct ggcgatcatg
     4021 agcggcctgg tcgagacgat cccttacctg gggccatacg ccgcaggcgc tatcgccgtc
     4081 ctggtagcg
//
LOCUS       DASUYC010000109         2688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_532780,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000109 DASUYC010000000
VERSION     DASUYC010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532780"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..701)
                     /locus_tag="VF276_01730"
     CDS             complement(18..701)
                     /locus_tag="VF276_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605609.1"
                     /translation="MNEQLTKFVLEDVPHQEVKTHPLEKLLAIAQARTVYSEPLTVGE
                     HTIITACEVTAVLGYGYGTPPAGAPNGTHPHNGTAPETNGALHEPPPAPSRAGQGGGG
                     GGSAYARPVAVIHIDPDGVWVDPVVDVVKISLGAFAVAATVARTVLRVALPPPAARGR
                     ALFGRPARLRPGKPAARVLRALLRRPARRRGLFSRFARARRTPPVRLMITQLRALIPA
                     PPVPRRHLA"
     gene            complement(741..1025)
                     /locus_tag="VF276_01735"
     CDS             complement(741..1025)
                     /locus_tag="VF276_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605610.1"
                     /translation="MTRLANWIEWRTITGAPVVAGEYTVRPQTQMLAIRPPAGGLVWS
                     RPVAVLAEHAGHTRRIRIVDVTRVAQLGLLIAGLGAGLVLRRIRPHAAGA"
     gene            1256..1477
                     /locus_tag="VF276_01740"
     CDS             1256..1477
                     /locus_tag="VF276_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605611.1"
                     /translation="MGRQAVGPALGQSRATDPHVIGMALVGMPFLALLPRATHVRLPG
                     VSHVLHNEQPVAVLAAMRPFLAAVRAMPA"
     gene            complement(1533..1889)
                     /locus_tag="VF276_01745"
     CDS             complement(1533..1889)
                     /locus_tag="VF276_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605612.1"
                     /translation="MRLLLIVALAGVLSVLGSATAAAQEMCPMTPTVAALRDCVTHAT
                     QEGLIDNAGVAGSLFAKIDAAQAAVDRGQPAVAATILNAFVHEVQAQAGRHIDATHAA
                     HMVMHAQAVIAALRAP"
     gene            complement(2230..2499)
                     /locus_tag="VF276_01750"
     CDS             complement(2230..2499)
                     /locus_tag="VF276_01750"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEX6605613.1"
                     /translation="MSNIVLEVPDISCAHCAHTITSALQGQPGVQSVQVDIPAKEVLL
                     SYDENALNLDQVGAILDEEGYPVAGSHAAGAAPAPRRNVIPLTGK"
BASE COUNT          356 a          913 c          974 g          445 t
ORIGIN      
        1 cggggtcggc gcgggactca ggcgaggtgc cggcgcggga ccggcggggc cgggatcagc
       61 gcccgcaact gggtgatcat caggcgcacc ggcggggtcc gccgcgccct cgcgaagcgg
      121 gaaaacaggc cccggcgccg ggccgggcgg cgcagcagcg cgcgcagcac cctggcggcc
      181 ggtttgccgg gccgcaggcg ggccgggcgc ccgaacaggg cgcgcccccg cgccgccggc
      241 ggcggcagcg ccacgcgcag caccgtgcgg gcgaccgtcg cggccacggc gaacgcgccc
      301 aggctgattt tgaccacatc gaccaccgga tccacccaga cgccgtccgg gtcaatgtgg
      361 atcacggcca ccgggcgcgc gtaggcgctg cccccgccgc ccccgccctg gccggcccgg
      421 ctgggtgcgg gcggcggctc gtgcagggcg ccgttggttt cgggtgcggt gccgttgtgc
      481 gggtgcgtgc cgttgggcgc cccggcgggc ggcgtgccgt aaccgtagcc gagcaccgcc
      541 gtgacttcgc acgccgtgat aatcgtgtgc tcgcccaccg tcagcggctc gctgtagacc
      601 gtgcgggcct gggcgattgc cagcaacttc tccagcgggt gcgtcttcac ctcctggtgc
      661 ggcacatctt ccaacacgaa cttcgtgagt tgctcattca ttgcggttgc tcctctcttg
      721 accgggggcg cggggagggg tcacgcgccc gccgcgtgcg ggcggatgcg gcgcaatacg
      781 aggccggctc ccagcccggc gatcagcagc ccaagctggg ccacccgcgt gacatcgacg
      841 atgcggatgc gcctggtgtg cccggcgtgt tccgccagca cagccacggg ccggctccag
      901 accaggccgc cggccggcgg gcggatcgcc agcatctgcg tctgcgggcg cacggtatac
      961 tcgccggcaa ctaccggcgc gccggtgatc gtgcgccact caatccaatt cgccaatcgt
     1021 gtcatccaag ctcctccttc cgcgtgcctg ccccggcgga tactcccagt atcatcttag
     1081 caccgccgcg ggcgcgcgcc ggtgagcatc cgttaaccgc gggtggcaga tcgcacgcgc
     1141 ggcggaaacc ggggggccga tgggtgccgc agccattcgc gcaatagcga gatgactcgg
     1201 cgcatcaacg ggcggggcgg tgttgggccg gcggcgggac cggcgggtgc cgtccgtggg
     1261 ccgccaggct gtcgggccgg ctctcggcca gagtcgcgcc actgatccac acgtcatcgg
     1321 catggccctg gtgggcatgc ccttcctcgc gctgctgccg cgggccacgc acgtccgcct
     1381 ccctggcgtc agccatgtgc tgcacaacga gcagccggtg gccgtgctgg ccgctatgcg
     1441 cccgttcctc gccgccgtac gcgccatgcc cgcctgatac ccacgacagt tcgtgcaccg
     1501 ctatgcgggt ggacgcgcct gggcgtgcgc ggtcagggcg cccgcaaggc cgcgatcacg
     1561 gcctgggcgt gcattaccat gtgggcggcg tgcgtggcgt cgatgtgtcg cccggcctgg
     1621 gcctgcactt catggacaaa ggcattgagg atggtggcgg ccaccgccgg ttggccccgg
     1681 tcgacggcgg cctgcgccgc gtcgatcttg gcgaagaggc tgccggccac gccggcattg
     1741 tcgatcaagc cctcctgcgt cgcgtgggtg acgcagtcac gtagcgccgc cacggtaggg
     1801 gtcattgggc acatctcctg ggcggcggcg gtggcactgc ccaacacgct cagcacgccg
     1861 gccagcgcga cgatcaatag cagtctcatc gttcgctcct ctcgataggt cagagatttt
     1921 ggacagggta tcgtttccgc gcattgcgga ttgtgccctc cagtatgcgc ggttgggtta
     1981 gcaacaaggg ttgcgccggg tgaataaccc acgccaatag gtgacagtat ggtgaatagg
     2041 ggatgagatg ccggcctatc gcccggtggc cggccctgta gcctgattag aggggtgcgg
     2101 cgggcctcgt ctgtctgatg gcctccaggc gggtcgtggg tgccggcctg tgcttaatgg
     2161 acttctccaa atgcgacggc ccggtgcacc attgcaccgg gccgcggtat atgcatgctg
     2221 ggctgcggct tacttgccgg tcagcgggat gacgttgcgc cggggggcgg gcgcggcgcc
     2281 ggcggcgtgg ctgcccgcca ccgggtagcc ttcctcgtcg aggatggcgc ccacctggtc
     2341 caggttcagc gcgttctcgt cgtagctgag gaggacctcc ttggccggga tgtccacctg
     2401 cacgctctgt acgccgggct ggccctgcag cgcgctggtg atggtgtgcg cgcaatgggc
     2461 gcacgagatg tccgggactt ccaacacgat gttcgacatg gaattgttgc tccttgtata
     2521 tttgctaatc gactatggcc ggtttgaaac cggcccctac aactcgtctg gtgccgggcc
     2581 gggttatggc cggtccctac aacggattga tcggcgggcc ggattatcgc cggtccctac
     2641 gatgttggcc ggacggtggg gccggggtgt ggccagcccc accgcgct
//
LOCUS       DASUYC010000110         3278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1236820, whole genome shotgun sequence.
ACCESSION   DASUYC010000110 DASUYC010000000
VERSION     DASUYC010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1236820"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VF276_01755"
     CDS             <1..483
                     /locus_tag="VF276_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015795528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX6605614.1"
                     /translation="NTLLVDLGLPGLQQNWLGDSSWALPSVMLMLVWFQIGYPLVLFM
                     AGMQRIDPEVYEAAAIDGATWLQRFYRITVPLLRPEIYVVVLTTTINALKTFGPIYAM
                     TRGGPGTATMVASYFSYKNFFQNANVGYGATMATVLTLIVMAITVVYIRVQTRQEENA
                     "
     gene            485..1357
                     /locus_tag="VF276_01760"
     CDS             485..1357
                     /locus_tag="VF276_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX6605615.1"
                     /translation="MATTTMSPGIKVAPPSLLRRLAGWIPLVLLSLLLLVMLFPLLLV
                     VISSFKTEAEYATNGPFALPQSLNFSVLSSTWQSTDYPVKLLNSAVISIGTAILATVL
                     SLFNAFAIAIGKIRGRAAFLSFFLLAITLPTEALIYPLYYMFKAVHLYDTQLSVILIT
                     ATLHSAFGTYLMTTVLNSFPKELLEAARLDGATRMQLLWRVVVPLSMPSLSVLFVFFF
                     IWTWNDFFLPLIFLISNAKQTVPLAVLSAQGEHGSVITTQAAASLLGILPCILFFIIF
                     QRTLTRGVAVGSIK"
     gene            1358..>3278
                     /gene="yicI"
                     /locus_tag="VF276_01765"
     CDS             1358..>3278
                     /gene="yicI"
                     /locus_tag="VF276_01765"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016362731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HEX6605616.1"
                     /translation="MKFTDGYWHMRPEVTAHWPAEAYETRVAPGTLTVYAPTKRAVTR
                     GATLNTPLITVRLSSPMPNVIRVQIYHFKGGHPRGPAFELEPLAVPEVLITDDADAAT
                     LTSGRLTARVAKGDWRLEFRDGDRVITGSGWRAMGFMDTPGGRFMVEQLDLGVGECVY
                     GLGERFTPFVKNGQVVDLWNEDGGTASEQAYKNVPFYLTNRGYGVFINHPERVSLEVA
                     SEKVERVQFSVPGEELDYFLIYGPSPKEVLERYTALTGRPALPPPWSFGLWLSTSFTT
                     DYDEATVTSFIQGMADRDLPFSVFHFDCFWMREFHWVDFLWDERVFPDPAAMLGRLKE
                     RGLRICVWINPYIAQQSVMFDEGMAGGYLVMRPNGDVWQWDKWQAGMGLVDFTNPAAR
                     QWFGDKLRALMDMGVDCFKTDFGERIPTDVVWFDGSDPFKMHNYYAYLYNKTVFEVLE
                     EKRGPGEAVVFARSATSGGQKYPVHWGGDNTATFESMAESLRGGLSLTLSGFGFWSHD
                     IGGFEQTATPDLYKRWCAFGLLSSHSRLHGSQSYRVPWLFDEEAVDVLRFFTRLKCRL
                     MPYLYGAARDAHETGVPVMRAMLLEFPDDPTCDYLDRQYMLGESLLVAPIFRPDGVVE
                     YYLPAGRWTNFLTGAV"
BASE COUNT          538 a         1067 c         1044 g          629 t
ORIGIN      
        1 aacaccctgc tggtggatct cgggctgccc ggtctgcagc aaaactggct gggcgacagc
       61 tcatgggcct tgccgtcggt gatgctgatg ctggtctggt tccagatcgg ctatcccctg
      121 gtgctcttca tggccggtat gcagcgcatc gatcccgaag tgtatgaagc ggcggccatc
      181 gacggggcca cctggctgca gcggttctac cgcatcaccg tgccgctgct gcgccccgaa
      241 atctatgtcg tggtgctgac gaccaccatt aacgccctga agaccttcgg cccgatttac
      301 gcgatgacgc gcggcgggcc gggcacggcc accatggtgg cctcgtattt ctcgtacaag
      361 aatttcttcc agaacgcgaa cgtcggctat ggcgctacca tggcgacggt gctgacgctg
      421 atcgtcatgg cgattacggt cgtctatatc cgcgtgcaaa cgcggcagga ggagaatgcc
      481 tgatatggct accaccacca tgtccccagg catcaaggtt gcgccaccca gcctgctccg
      541 ccgcctggcc ggttggatcc cgctggtgtt gctctccctg ctgctgctgg tgatgctgtt
      601 cccactgctg ctggtcgtga tcagctcctt caagaccgaa gccgagtacg caaccaatgg
      661 gccgtttgcc ctgccgcaga gcctcaactt cagcgtgctc agcagcacct ggcagagcac
      721 cgattacccg gtcaagttac tgaacagtgc cgtcatcagc atcggcacgg ccatactggc
      781 cacggtgctg tcgctgttca acgccttcgc catcgccatc ggcaagatcc gggggcgcgc
      841 cgccttcctg tcgttcttcc tgctggcgat cacgctgccg accgaggcgc tgatctaccc
      901 tctgtactac atgttcaagg ccgtccacct ctacgacacg cagttgtcgg tcatcctgat
      961 cacggcgaca ctgcacagcg ccttcggcac ctacctgatg accacggtgc tcaactcgtt
     1021 cccgaaagaa ctgctggagg cggcgcgact cgacggcgcc acgcggatgc aactgctctg
     1081 gcgggtggtc gtgccgctca gcatgccgtc gctgtcggtg ctgtttgtgt ttttctttat
     1141 ctggacctgg aacgatttct tcctgcccct gatcttcctg atctcgaacg cgaagcagac
     1201 cgtgccgctg gccgtcttgt cggcacaggg cgaacacggg tccgtcatca caacgcaggc
     1261 cgcggcgtcg ttgctcggca tcctgccgtg catcctcttc ttcatcattt tccagcggac
     1321 gctgacgcgg ggcgtggctg tggggagtat caaatagatg aaattcaccg atggatactg
     1381 gcacatgcgg cccgaggtga cggctcattg gccggcggag gcgtatgaga cgcgggtcgc
     1441 gcccggcacg ctgacggtgt atgcccccac caagcgggcc gtcacccgtg gcgcgaccct
     1501 caacacgccg ctgatcacgg tgcgcttgtc ctcgcccatg ccgaacgtca tccgtgtgca
     1561 gatctaccac ttcaagggcg ggcacccgcg cgggccggcc tttgagttgg agccgctagc
     1621 ggtgcccgaa gtgctcatca ccgacgacgc ggacgcggcg acgctgacca gcggccggtt
     1681 gacggcgcgc gtggcgaagg gcgattggcg cctggagttc cgcgacggcg accgggtgat
     1741 caccgggagc ggctggcggg cgatgggctt catggacacg cccggcgggc gcttcatggt
     1801 cgagcaactc gacctgggcg tgggcgaatg cgtttacggc ctcggtgagc gcttcacccc
     1861 cttcgtcaag aacggccagg tggtcgatct gtggaacgag gacggcggca cggccagcga
     1921 gcaggcatac aagaacgtcc cgttctacct gaccaatcgc ggctacggcg tcttcatcaa
     1981 ccacccggag cgcgtctcgc tcgaagtggc gtcggagaaa gtcgagcggg tgcagttcag
     2041 cgtgcccggc gaagaactgg actacttcct catctacggg ccgtcgccga aagaggtgct
     2101 ggagcgctac acggcgctga ccgggcgccc ggctctgccg ccgccgtggt cgttcggcct
     2161 ctggctcagc acttctttca caaccgacta cgacgaggcg accgtcacca gctttatcca
     2221 gggcatggcc gaccgcgacc tgccgttcag cgtgttccac ttcgactgct tctggatgcg
     2281 cgagttccac tgggtcgatt tcctgtggga cgagcgcgtc ttccccgacc cggcggcgat
     2341 gctcgggcgg ctcaaagagc gcggcctgcg catctgcgtc tggatcaacc cgtacatcgc
     2401 ccagcagtcg gtgatgttcg acgagggcat ggcgggcggc tacctggtca tgcggcccaa
     2461 cggcgacgtg tggcagtggg ataagtggca ggcgggcatg gggctggtcg atttcaccaa
     2521 cccggcggcc cggcagtggt tcggcgacaa gctgcgcgca ctgatggaca tgggcgtgga
     2581 ttgcttcaag accgacttcg gcgagcgcat cccgaccgac gtagtctggt tcgacggctc
     2641 cgacccgttc aagatgcata actactacgc ctacctgtac aacaagacgg tattcgaggt
     2701 gctggaagaa aagcgcggcc ccggcgaagc ggtcgtcttt gcccgctcgg cgaccagcgg
     2761 cgggcagaag taccccgtcc actggggcgg cgacaacacg gccacgttcg agtcgatggc
     2821 cgagagcctg cgcggcggcc tgtcgctgac cctgtcgggc ttcggcttct ggagccacga
     2881 catcggcggg ttcgagcaga cggccacgcc cgatctgtac aaacgctggt gcgccttcgg
     2941 cctgctgtcg tcgcatagcc gcctgcacgg cagccagtcc taccgcgtgc cctggctgtt
     3001 cgacgaagaa gcggtggacg tgctgcgctt cttcacccgg ctcaagtgcc ggctcatgcc
     3061 atacctgtat ggcgcggccc gcgatgcgca cgagacgggc gtgcccgtca tgcgagccat
     3121 gctgctggag ttcccggacg atcccacctg cgattatctc gaccggcagt acatgctggg
     3181 cgagtcgctg ctggtcgccc cgattttccg gcccgatggg gtcgtggagt actacctgcc
     3241 tgcggggcgc tggaccaact tcctgaccgg cgcggtgg
//
LOCUS       DASUYC010000111         2423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1591118, whole genome shotgun sequence.
ACCESSION   DASUYC010000111 DASUYC010000000
VERSION     DASUYC010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1591118"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1200
                     /locus_tag="VF276_01770"
     CDS             <1..1200
                     /locus_tag="VF276_01770"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEX6605617.1"
                     /translation="RRVPLPQAATLDRAAYVALLHTALDAANAVPAAADAPERPALLA
                     RARAVLPASAVVRDAGGVLAEVDFGPVRAALAKDPPDLAGARRYLTALIGLLDPGSLP
                     SPTATPTPLQTPSAAQAALALTPVYLPPTGAGGPPIAAGDAGSRLERVLADPRFQPDE
                     GGGIQQALARALEPLVGTLLAMPTIQRNLLIAAVAGLLVAFMIYAGYREAPWSRQRYR
                     ATVAGGGLATFVLVFLLLTYGLNALGLLLAIPFLAPVLGALGVLVAVGVAVFGWLGLR
                     RARAPQHTRVASPFAAEAGWTAAQARAAAEAAAGAQDYRKAIRYRYLATLLALDEAGH
                     VRFDPALTNHEYLQRAPGPLRDPLQPLVGIFERLWYGGFPATAEDYRAYQALAARAES
                     VPAEVAS"
     gene            1197..2384
                     /locus_tag="VF276_01775"
     CDS             1197..2384
                     /locus_tag="VF276_01775"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HEX6605618.1"
                     /translation="MSRRWGDRLIFALLLVVLLGVSVISFLGDTARQGQAELRGSTLS
                     AAPPGTLALYRWLEDAGYPVTRIQGSARFADALRDTDLLFVLNPQTQFSPEQLAALEA
                     WMGAGGVLVLSIEGSEGLLNPITARLGAELSPILPPSAGALPLRQPLWTDPPVRTVSV
                     QTSWQLDLKGDDAIPLLSAGSGPLMIAQPRGRGRLILLSTTWPFTNAGLREADNRWLA
                     WNLAASGAGRRIAFDEIHHGYTGGDLRALVLSRPWGWALIYAAGVLVVGLLLAGRRLG
                     PPLVPMAAGGRRGAAEYVAALAGLFQRAGRTDWVAGRYRAEFRRALAAPYGLDATAPA
                     GALAAAFAAAHHRPVDVSALTALLAELDQAATPAGRGGKATGDAALLRLVRRAEAFRN
                     DIM"
BASE COUNT          260 a          903 c          904 g          356 t
ORIGIN      
        1 cgccgcgtcc cgctgccgca agccgcgacg ctcgaccgcg ccgcctacgt ggccctgctg
       61 cacactgcgc tcgacgcggc caacgccgtg cccgccgccg ccgatgcgcc cgagcggccc
      121 gccttgctgg cgcgcgcccg cgccgtgctg ccggcaagcg cggtcgtgcg cgatgcgggc
      181 ggggtgctgg ccgaggtcga tttcgggccg gtgcgcgctg cgctggcgaa agacccgccc
      241 gacctggctg gagcgcgccg ctacctgacc gcgttgatcg gcctgctgga ccccggcagc
      301 ctgccctcgc cgacggcgac ccccacgccg ctgcaaaccc ccagcgccgc gcaggccgcc
      361 ctggcgttga ccccggtgta tctgccgccc acgggcgcag gcgggccgcc gattgccgcg
      421 ggcgatgccg ggagccgcct ggagcgcgtg ctggccgacc cgcgctttca gccggacgag
      481 ggagggggca tccagcaggc cctggcgcga gcgctggagc cgctggtcgg taccctgctg
      541 gcgatgccca ccatacagcg caatttgctg atcgccgcgg tggccgggct gctcgtcgcc
      601 ttcatgatct atgccggcta tcgggaagcg ccctggtccc gccaacgcta ccgggccacc
      661 gtggcgggtg gcgggttggc gactttcgtg ctggttttcc tgctgctgac ctacggcctg
      721 aacgcgcttg gcctcctcct ggcgatcccg ttcctggcgc cggtgctggg ggcgctgggg
      781 gtgctggtcg ccgtgggcgt ggccgtgttt gggtggctgg gcctgcgccg ggcgcgcgcc
      841 ccgcagcaca cgcgggtggc ctcgccgttc gccgccgaag cgggctggac ggcggcccag
      901 gcgcgggcgg cagccgaggc cgccgccggg gcgcaggact atcgcaaggc catccgctat
      961 cgctacctcg ccacgctgct cgcgctcgac gaggccgggc atgtgcgctt cgatcccgcg
     1021 ctgaccaacc atgagtattt gcagcgcgcg cccggcccgc tgcgcgaccc gttgcagccg
     1081 ctggtcggca tcttcgagcg gctctggtat ggcggcttcc cggccacggc ggaggactac
     1141 cgcgcctacc aggcgctggc cgcccgcgcc gagagcgtgc ccgcggaggt cgcgtcgtga
     1201 gccgccgctg gggcgaccgg ctgatcttcg ccctgctgct cgtggtgctg ctcggcgtgt
     1261 cggtcatctc gttcctgggg gacacggcgc gccaggggca ggccgaactg cgcggcagca
     1321 cgctgagcgc ggccccgccc ggcacgctgg ccctctatcg ctggctggaa gacgcgggct
     1381 atcccgtcac tcgtatccag ggcagcgcgc gcttcgccga tgccctgcgc gacacggatc
     1441 tgctctttgt gctgaacccg cagacgcagt tcagccccga gcagttggcg gcgctggagg
     1501 cgtggatggg cgcgggcggc gtgctggtgt tgagcatcga gggcagcgaa gggctgctca
     1561 acccgatcac cgcccggctg ggcgccgagt taagcccgat cttgccgccg agtgccggcg
     1621 ccctgccgct gcgccagccg ctgtggaccg acccgcccgt gcgcacggtc agcgtgcaga
     1681 cctcgtggca actcgatctc aagggcgacg acgcgatccc gctgctgagc gccggcagcg
     1741 ggccgctgat gatcgcgcaa ccccgcgggc gcgggcggct gatcctgctc tcgaccacct
     1801 ggcccttcac caacgccggg ctgcgcgaag ccgataatcg ctggctggcc tggaacctgg
     1861 cggcatccgg cgcggggcgg cgcatcgcct tcgacgagat ccaccacggc tacaccgggg
     1921 gcgacttgcg cgccctggtg ttgagcaggc cctggggctg ggcgctgatc tacgcggcgg
     1981 gggtgctggt ggtgggcctg ctgctggccg ggcggcggct gggcccgccg ctggtcccca
     2041 tggccgcggg cgggcgccgg ggcgccgccg agtatgtggc ggccctggcc gggctgttcc
     2101 agcgcgccgg gcgcaccgac tgggtggcgg gccgctatcg cgccgagttc cgccgggcgc
     2161 tggccgcccc ctatggcctg gacgcgacgg cccccgccgg tgccctggcc gccgcctttg
     2221 ccgccgctca ccatcgtccg gtggatgtgt ccgcgctgac cgcgctgctc gctgagctgg
     2281 accaggccgc gacccccgcc gggcggggcg gcaaggccac cggcgacgcg gcgctgctgc
     2341 ggctcgtccg ccgcgccgag gcgttccgca acgacattat gtgagccgcc caggggcggg
     2401 gaactatact atggccttac atc
//
LOCUS       DASUYC010000112         5625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1225032, whole genome shotgun sequence.
ACCESSION   DASUYC010000112 DASUYC010000000
VERSION     DASUYC010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5625
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225032"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="VF276_01780"
     CDS             <1..925
                     /locus_tag="VF276_01780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX6605619.1"
                     /translation="PFPPLTSWPAPPGWPETADRVAATLRGRFDLAILPRPDDPMTGA
                     LVAAAGIPIRLGYACPRTAPFLTMALPAPPRRHVAALACDLVRAAAHCFGAQTPDAAM
                     PAPRYFVPTPADEAEAATAQAQVRARCGPHPILIHPAAGWPLKSWPLARWAALAAGLR
                     ARYGVCPLVVCGPDAREIESAIIDLSRGAACGLGHALSLGALAAVQQQARLVIGIDSG
                     PLHLAALLGTPVISLFGPADPREFRPWCPPDRYRIVRVRLACSPCGTLMDPPCGATRD
                     PACMTGITVEAVLAAVGELHAQTLLPAGVKE"
     gene            928..1755
                     /locus_tag="VF276_01785"
     CDS             928..1755
                     /locus_tag="VF276_01785"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6605620.1"
                     /translation="MVPSEQSSTPSALIRADQPWGDAILARLYDAFPFAADLPLYLEL
                     AAAQGGRVLEVACGSGRVLVPLAQAGHRVTGVDASPHMLAVAREKLAAAGPAAQANAR
                     LVQADMRSFELDEQFDLAVIAVKSIAYLTERADQERTLARVAAHLRPGGLLAIDLMHP
                     APAWLLEPPGSLRQDLVHDVAAQGITVARTEAVVSTDLAAQVRVIRSAYEIVAADGTV
                     TKRFVEWPYRYIYRFEAEHLLERAGFVIEALYGGYQREPFSSESRTMLFLARRPGAS"
     gene            complement(1686..2843)
                     /locus_tag="VF276_01790"
     CDS             complement(1686..2843)
                     /locus_tag="VF276_01790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6605621.1"
                     /translation="MTRILRVALLTLGDPGTLTGGYLYHLRMAQLAPQHDARIVFVSF
                     PERPFPLATIDAPAVIAGARRRGVDVVVLDSIAAAYLGPWLAGRAPHWPLLAMLHQPP
                     GGIDYGPARTTVQAVLDRWAYRHARRLLVASESLADELTAQGIPRDRVRVVPPGRDVA
                     AAPGAPPGDLRQGRRAALLCVGNWVARKGILNLLDAVARLPPDAATLHLAGDDATEPD
                     YAARVRARLRLPDLAGRVVVHGRLALAAVAALYAAADVFVLPSLKEPYGTVYGEAMAA
                     GLPVVGWRAGNLPYLADDGGEGLLVPPGDIGGLARALARLADDAPLRRRMGEAARRRA
                     LARPTWAESAGLFFAAIREALRESGGKPDQDAPGRRARNSMVRDSDENGSR"
     gene            2833..3924
                     /locus_tag="VF276_01795"
     CDS             2833..3924
                     /locus_tag="VF276_01795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6605622.1"
                     /translation="MRVIHVAPTPFGRAGLLGGGERYPLELARALAAEVDCELISFGP
                     RPLRIREPGGLRVRILRAPLYLHGHPAHPVAPALPAALRRADLVHTHHLRSTPSRVAA
                     LAARARRQKLVVTDHGLRGGDWAGLLPRLFDRFLAVSAYSARELHSPPRRTTVIYGGA
                     DPQRYRPDPSARRDGVLFVGRITPHKGVDRLIAALPPGARLQIAGSTGHDPHPPERDY
                     PQLLQRLAAGRDVAFLGPVADADLPVLYRQARVLVLPSVHSTCYGRAIRVSELLGLVV
                     LEAMASGTPVIGSRLGGIPEIVEHGVTGFLVEPGNVAELRARLAELLHDPARAARMGR
                     NARDLVLERFTWRKCAERCLTVYSDLVRA"
     gene            complement(3944..5416)
                     /gene="betC"
                     /locus_tag="VF276_01800"
     CDS             complement(3944..5416)
                     /gene="betC"
                     /locus_tag="VF276_01800"
                     /EC_number="3.1.6.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR03417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline-sulfatase"
                     /protein_id="HEX6605623.1"
                     /translation="MAASPNILLIMADQLTPFLMGAYDHPVVQTPHLDALVADGVRFD
                     AAYTPYPLCAPARAAFLTGKYASSLGVYDNAAVLHADEPTLAHYLTNAGYDTALSGKM
                     HFIGPDQLHGFRRRLTTDVFPANIAWVPTRDPVTGRFPGGGHARLYVAPNVGVRPWTK
                     FLAYDEETQFRALEYIRERGRAAPDEPFFLIASYHHPHDPFHVTQELWDLYEGADIAI
                     PTYPPNLAETYSALDRWLNEVHETDQVDLGNPDSLYQLRRAYYGLVTYIDRKVGELLA
                     ALDQTGQRENTVVIFTSDHGDMLAERGMVQKRCFYEWSARVPLIIQFPHGAYAGRKVA
                     EPVSLLHLAPTLLDLAGVPPVARLPMDGTSLLPLLDGAETGERPVLSEYHVEKVYAPC
                     FMVRQGRYKYVYIHGWGSQLFDLDADPGEWQNLAGRPGLQAVEAALHGRILAQFDPDG
                     IAEAGEASVARRVVIKEALARNGTHWDYTPQFDGTKQYVR"
     gene            complement(5428..>5625)
                     /locus_tag="VF276_01805"
     CDS             complement(5428..>5625)
                     /locus_tag="VF276_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017238123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HEX6605624.1"
                     /translation="NPQYDTGHLARVARLEALAAAHLPGVYLAGSAYRGIDVAATVRE
                     ARRAAEQILAVRQADSPGTSA"
BASE COUNT          760 a         2066 c         1986 g          813 t
ORIGIN      
        1 cccgttcccg ccgctgacat cctggcccgc cccgcccggc tggccggaga cggcggaccg
       61 cgtcgccgcc accctgcgcg ggcgctttga cctcgccatc ctgccccgcc ccgatgaccc
      121 gatgacgggg gcgctggtgg cggcggcggg catccccatc cgcctcggct atgcctgccc
      181 gcgcacggcc ccgttcctga cgatggcact cccggccccg ccccggcggc atgtcgcggc
      241 cctggcctgc gatctggtgc gggccgccgc gcactgcttc ggcgcgcaga cccctgatgc
      301 cgccatgccg gcgccccgct attttgtgcc caccccggcg gacgaggcag aagcggccac
      361 ggcgcaggcg caggtgcgcg cccggtgcgg cccgcatccg atcctgatcc atcccgccgc
      421 gggctggccg ctcaaaagct ggccgctggc gcgctgggcg gcgctggccg ccgggttgcg
      481 cgcccgctac ggcgtgtgcc cgctggtggt ctgcgggccg gacgcccgcg agatcgagtc
      541 cgccatcatc gacctgagca ggggcgcagc gtgcggcctg ggccatgcgc tctcgctagg
      601 cgcgctggcg gcggtgcagc agcaggcgcg cctggtgatc ggcatcgaca gcggcccgct
      661 gcatctggcg gcgctgctcg gcacgccggt gatcagtctg ttcggccccg ccgatccgcg
      721 cgagttccgg ccctggtgcc cgccggaccg ctaccgcatc gtgcgcgtgc ggctggcgtg
      781 cagcccgtgc ggcacgctca tggacccgcc gtgcggcgcg acccgcgacc ccgcctgcat
      841 gaccggcatc accgtggagg cggtgctggc ggcggttggc gagttgcacg cgcaaacgct
      901 cttgcctgcc ggtgtgaagg agtaagcatg gttccctctg agcaatcgtc cacccccagc
      961 gcgctgatcc gggccgatca gccgtggggc gacgccatcc tggcccgcct gtatgacgcc
     1021 tttcccttcg cggcggacct gccgctgtac ctggaactcg ccgcggcgca gggcgggcgg
     1081 gtgctggagg tggcctgcgg cagtgggcgc gtgctggtac ccctggccca ggcggggcac
     1141 cgggtgacgg gcgtcgatgc ctcgccccat atgctggccg tggcgcgcga aaagctggcc
     1201 gccgccggcc ccgccgcgca ggccaatgcg cgcctggtgc aggccgacat gcgctcgttc
     1261 gagttggacg agcagttcga cctggcggtg atcgccgtca agagcatcgc ctacctgacc
     1321 gaacgcgccg accaggagcg gaccctcgcc agggtggcgg cgcacctgcg gcccggcggc
     1381 ctgctggcaa tcgacctcat gcacccggcg ccggcctggc tgctcgaacc tcccggcagc
     1441 ttgcggcagg acctcgtcca cgacgtggcc gcacagggca tcacagtggc ccgcaccgag
     1501 gccgtggtca gcaccgacct cgccgcgcag gtgcgggtca tccgctcggc ctacgagatc
     1561 gtcgccgccg acggcacggt cacgaagcgc ttcgtggagt ggccctatcg ctatatctat
     1621 cgcttcgagg ccgaacatct gctggagcgc gctgggtttg tcatcgaggc cctctatggc
     1681 ggctatcagc gcgagccgtt ttcgtcggaa tcgcgcacca tgctgttcct ggcccgccgg
     1741 cccggcgcgt cctgatccgg tttcccgccg ctttcgcgca gcgcttcgcg gatggcggcg
     1801 aagaacaggc ccgccgactc ggcccaggtg ggtcgggcca gggcgcggcg gcgggctgcc
     1861 tcccccatgc gccggcgcag cggcgcgtcg tccgccagcc gtgccagcgc ccgcgccagc
     1921 ccaccgatat cgccgggcgg caccagcagc ccttcgccgc catcgtccgc cagatacggc
     1981 aggttgcccg cccgccagcc gaccaccggc agcccggcgg ccatcgcctc accatagacc
     2041 gtgccgtacg gctctttcag gctcggcagc acgaacacgt ccgcggcggc gtagagcgcg
     2101 gccacagcgg ccagggccag ccgcccatgc acgaccaccc gcccggcgag gtcgggcagg
     2161 cgcagccggg cgcgcacgcg ggccgcatag tcgggctcgg tcgcatcgtc gcccgccagg
     2221 tgcagcgtgg cggcatcggg cggcaggcgg gcgaccgcat cgagcaggtt gaggatgccc
     2281 ttgcgcgcca cccagttgcc cacgcacagc agggcggccc ggcgcccctg ccgcagatcg
     2341 ccgggcggcg caccgggagc cgccgccacg tcgcgccccg gcggcaccac ccgcacccga
     2401 tcccgcggta tgccttgtgc agtgagttca tcggccagcg attcgctcgc caccagcaag
     2461 cggcgggcgt gccggtaggc ccagcggtcg aggacggcct ggacggtggt gcgcgccggg
     2521 ccgtagtcga tgccgccggg gggctggtgg agcatggcga gcagcggcca atgcggcgcc
     2581 cgcccggcca gccacggccc caggtaggcg gcggcgatgc tgtcgagcac caccacgtcc
     2641 acgccccggc ggcgcgcccc ggcgatcacg gcgggcgcgt cgattgtcgc cagcgggaag
     2701 ggccgttcgg ggaaggacac aaacacgatc cgggcgtcgt gctgcggcgc cagttgcgcc
     2761 atccgcagat gatagagata acccccggtc agcgtgccgg gatcgccgag cgttagcaat
     2821 gcgaccctaa gtatgcgtgt catccacgtc gcccccacac cctttggccg cgccggcctg
     2881 ctcggcggcg gcgaacgcta tccgctggag ttggcgcggg cgctggccgc cgaagtcgat
     2941 tgcgaactga tcagctttgg gccgcgcccc ttgcgtatcc gcgaaccggg cggcctgcgc
     3001 gtgcggatct tgcgggcgcc cctgtacctg cacggccacc ccgcccaccc ggtggcgccc
     3061 gcgctgcccg ccgccctgcg ccgcgccgac ctggtgcata cccatcacct gcgcagcacg
     3121 cccagccggg tggccgcgct ggccgctcgc gcccgccgcc aaaagctggt ggtgacggac
     3181 cacggcctgc gaggcggcga ctgggccggg ctgttacccc gccttttcga ccgcttcctg
     3241 gcggtctccg cctattcggc ccgcgagttg cacagcccgc ctcgccgcac cacggtgatc
     3301 tacggcgggg ccgacccgca gcgctaccgg cccgacccgt ccgcccggcg cgacggcgta
     3361 ttgttcgtgg gccggatcac gccccacaaa ggcgtggatc gcctgatcgc cgccctgccc
     3421 cccggcgcgc gcctccagat cgccgggtcc accggccacg acccgcaccc gccggagcgg
     3481 gattatccac agctattgca acggctggcc gccgggcggg atgtggcctt cctggggccg
     3541 gtggccgacg ccgatctgcc tgtgctctac cggcaggcgc gcgtgctggt gctgccgtcc
     3601 gttcacagca cctgctatgg ccgggccatc cgggtctcgg aactgctggg cctggtcgtg
     3661 ctcgaagcga tggcgagcgg caccccggtg attggcagcc gcctgggcgg catcccggag
     3721 atcgtcgagc acggcgtcac cggcttcctg gtcgagcccg gcaacgtggc cgaactgcgg
     3781 gcccgcctgg cggaactcct gcacgacccc gcccgcgccg cgcgcatggg acgcaacgcg
     3841 cgagacctgg tcctggaacg tttcacctgg cgcaaatgcg ccgaacgctg cctgaccgtc
     3901 tacagcgatc tcgtgcgggc ttgaaccggg ccgcgcccgg cgctcagcgc acgtattgct
     3961 tcgtgccgtc gaactgcggc gtatagtccc agtgcgtgcc gttgcgcgcc agcgcctcct
     4021 taatcacgac ccgccgggcc acactcgcct cccccgcctc cgcgataccg tccgggtcga
     4081 actgcgccag gatccggcca tgcagcgccg cctccaccgc ttgcaggccc ggccgcccgg
     4141 ccaggttctg ccactcgccg ggatcggcat ccaggtcgaa caactggctg ccccagccgt
     4201 ggatatagac atacttatac cggccctgcc gcaccatgaa acacggcgca tatacctttt
     4261 ccacatggta ttccgacagg acggggcgct ctcctgtctc ggcgccgtcc agcaggggca
     4321 gcaggcttgt gccgtccatc ggcaggcggg ccaccggcgg cacgcccgcc aggtcgagca
     4381 acgtgggcgc caggtgcagc agggagaccg gctccgccac cttgcgcccc gcgtaggcgc
     4441 cgtgcgggaa ctggatgatc aacggcacgc gcgccgacca ctcatagaag cagcgcttct
     4501 gcaccatgcc ccgctcggcc agcatatcgc cgtggtcgct ggtgaaaatg accacggtgt
     4561 tctcgcgctg gccggtctgg tctagcgccg ccagcagttc gcccactttg cggtcgatat
     4621 aggtcaccag cccatagtag gcgcggcgca actggtagag actatccggg ttgccgagat
     4681 cgacctgatc ggtctcgtgg acctcgttga gccagcggtc cagcgccgaa taggtctccg
     4741 cgaggttcgg cggataggtg gggatggcga tgtcggcgcc ctcgtacagg tcccaaagct
     4801 cctgcgtgac gtggaaggga tcgtgggggt gatggtagga agcgatgagg aagaatggtt
     4861 cgtccggcgc ggcgcggccc cgttcgcgga tgtactccag tgcccggaac tgggtctctt
     4921 cgtcataggc caggaacttg gtccagggcc gcacgccgac gttgggcgcg acatagagcc
     4981 gggcgtggcc gccgccgggg aagcgccccg tgacgggatc gcgggtgggc acccaggcga
     5041 tgttggcggg aaagacatcc gtggtcaggc ggcggcggaa gccatgcaac tggtcggggc
     5101 cgatgaaatg catcttgccg ctcagcgccg tgtcgtagcc ggcgttggtc aggtagtggg
     5161 ccagcgtcgg ctcgtcggcg tgcagtacgg cggcgttgtc gtagacgccg agggacgagg
     5221 catatttgcc ggtcaagaac gcggcgcgcg ccggggcaca gagcggatag ggcgtgtaag
     5281 ccgcgtcgaa gcgcacgccg tcggccacca gcgcgtccag gtggggcgtc tgcaccacgg
     5341 ggtggtcata ggcgcccatc aggaagggag tcaactggtc ggccataatc agcaagatgt
     5401 tcggggacgc agccatgaac agtactccta tgcggacgtg ccgggtgaat cggcctgccg
     5461 gacggccagg atctgctcgg cggcccgccg ggcctcgcgg acggtggccg ccacgtcgat
     5521 gccccgatag gcgctgccgg cgaggtagac gcccggcagg tgggccgccg ccagggcttc
     5581 gagccgcgcc acccgcgcca ggtggccggt gtcgtactgc gggtt
//
LOCUS       DASUYC010000113         2124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_69753,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000113 DASUYC010000000
VERSION     DASUYC010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69753"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="VF276_01810"
     CDS             complement(<1..733)
                     /locus_tag="VF276_01810"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEX6605625.1"
                     /translation="MEPAESPAYAVRLPDFEGPLDVLLRLIERQELEISKVSLALVAD
                     QFLEYVEALPAPEPGVLAAFMVVAAKLLLIKSQALLPRPPPPPAPDEDEEDVGAELVR
                     RLQEYRAIQQTARELREREAAGLHSYVRPATLARQRRPVQRPPTLGLEGVSLGQLTRL
                     VNRRLQLALPFAEPPALIPHEITLAEKVAELQEHLDAAGPGGTVGVWPLLVAARSRAE
                     IVITFMAVLEILRRRLAVAEQDAAFG"
     gene            complement(737..1309)
                     /locus_tag="VF276_01815"
     CDS             complement(737..1309)
                     /locus_tag="VF276_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014794378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605626.1"
                     /translation="MQAWPLRGLVARLPYRVRQFLTANLAPVAPAEIEAALAQARLPE
                     PAARLFRAMPRPYQRHALNVAARLQHAGHSDPILLSAALLHDMGKWDPASGRKVPVVH
                     RSIVALITWCAPGRQLLRRLVAGPPPMRSWRYPWHLQQHHPELSARLAEAAGVDPDVV
                     ALVRYHQDGAGIPARLRPTLALLQQADDQE"
     gene            complement(1324..1989)
                     /locus_tag="VF276_01820"
     CDS             complement(1324..1989)
                     /locus_tag="VF276_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX6605627.1"
                     /translation="MFGNLDPVRIIGVMLGFIIGTTIHEFMHAYSAVMLGDNSPRRAG
                     RVTLNPVAHFDPLGFFMFVLLALGLGFFAWGRPVMVNPSALRGGRKGMAIVALAGPAS
                     NLVIATVLGLPFRVGVADQLPSQVGDVIGYIVFVNVLLFAFNLIPLPPLDGFTVMTGL
                     VSNYWAMLMEPLRRYGVALLMLLLFVPYFLPGAQNFNILARVLGPVMAVINAAIGGGR
                     LPV"
BASE COUNT          356 a          714 c          756 g          298 t
ORIGIN      
        1 cgccgaacgc ggcatcctgc tcggccacgg ccagccggcg gcgcaggatt tcgagcacgg
       61 ccataaaggt gatgacgatc tcggcacgcg agcgggcggc gacgagcagc ggccagacgc
      121 cgaccgtgcc gcccggcccg gcggcatcca ggtgctcctg tagttcggct accttctcgg
      181 ccagggtgat ctcatggggg atgagggccg gcggctcagc gaagggcagg gccagttgca
      241 ggcgccgatt gaccagccgg gtcaactggc ccagcgagac cccttcgagg cccagcgtgg
      301 gcgggcgctg caccgggcgg cgctgccggg ccagggtggc ggggcgcacg tagctgtgca
      361 gccccgcggc ctcgcgttcg cgcaactcgc gcgccgtctg ctggatggcg cggtactctt
      421 gcagccggcg caccagttcg gcgcccacat cttcctcgtc ctcgtcgggc gccgggggcg
      481 gtggcgggcg gggtagcaac gcctgggact tgatcagcag cagcttggcc gcgacgacca
      541 tgaacgcggc cagcacgccc ggttcgggcg cgggcagcgc ctcgacatat tccaggaact
      601 gatcggccac cagcgccagc gagaccttgc tgatctccag ctcctggcgc tcgatcaggc
      661 gcagcagcac gtcgagcggc ccctcgaagt cgggcaggcg gacggcgtac gcgggcgact
      721 cggccggttc cacgccctac tcctggtcgt cggcctgctg caacagggcc agcgtgggcc
      781 gcagccgggc cgggatgccg gcgccatcct ggtgatagcg caccagggcc accacatcgg
      841 gatcgacccc ggcagcttcc gccaggcgcg cgctcagttc ggggtgatgt tgctgcaagt
      901 gccaggggta gcgccagctt cgcatcgggg gcggcccggc caccagccgg cgcagcaact
      961 gccgccccgg cgcgcaccag gtgatgagcg ccacgatgga gcggtggacc accggcacct
     1021 tgcgcccgct ggccgggtcc cacttgccca tgtcatgcag cagggcagcg ctcagcaaga
     1081 tgggatcgct gtggccggcg tgctggagcc gggcggcgac gttgagtgcg tggcgctggt
     1141 aggggcgggg catggcgcgg aacaggcgcg ccgccggctc gggcaaccgc gcctgggcga
     1201 gcgccgcctc gatttccgcc ggggccacag gcgccaggtt cgcggtcagg aactgccgca
     1261 cgcgataggg tagccgggcg accagtccgc gcaggggcca tgcttgcatg tcggtcgcgg
     1321 ccatcagacg ggtaaccggc ccccgccgat tgccgcgttt atgaccgcca tcaccgggcc
     1381 gagtacgcgg gcaaggatat taaagttctg cgcgcccggc aggaagtatg gtacgaacaa
     1441 cagcagcatc aacagggcca caccgtagcg gcgcaacggt tccatcaaca tggcccagta
     1501 gttcgacacc agcccggtca tgaccgtgaa gccgtcgagg ggcggcagcg ggatcaggtt
     1561 gaacgcaaac agcaggacgt tcacgaacac gatatagccg atcacatcgc ccacctggga
     1621 cggcaactga tcggctacgc cgacccggaa cggcaggccg agcaccgtgg cgatcaccag
     1681 gttgctggcg gggccggcca gggccacgat cgccatgcct ttgcgcccgc cgcgtaacgc
     1741 gctggggttg accatgactg ggcggcccca ggcgaaaaag cccagcccca gcgcgagcag
     1801 cacaaacatg aagaacccca gcgggtcgaa gtgcgccacc gggttcaagg tgacgcgccc
     1861 ggctcggcgg gggctgttgt cgcccagcat caccgccgag taggcgtgca taaactcgtg
     1921 gatcgtcgtg ccgatgatga aaccaagcat gaccccgata atacggacgg ggtccagatt
     1981 accaaacatc catgggcctt tggctagtct cgctgttcga gatccttttt cacatcgtcg
     2041 agcgccgtct tcaactgctc gacccgcgct tgcaggtcgc tggtatcggg caggggttcg
     2101 ggcaggtcag gggccgccgg tggc
//
LOCUS       DASUYC010000114         4393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1348147, whole genome shotgun sequence.
ACCESSION   DASUYC010000114 DASUYC010000000
VERSION     DASUYC010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1348147"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(283..678)
                     /locus_tag="VF276_01825"
     CDS             complement(283..678)
                     /locus_tag="VF276_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6605628.1"
                     /translation="MDNGTQPHAASRILVVDDDADFFALLYDALTHTMPNRAIVGVRS
                     AEAALAELERGPFDLILVDYRLEGRNGLALLHDLRALAPALPVIMITGYPTQALRQQA
                     EALGVSGFLIKPVFVPDLRRAVQAALAGR"
     gene            795..3206
                     /locus_tag="VF276_01830"
     CDS             795..3206
                     /locus_tag="VF276_01830"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HEX6605629.1"
                     /translation="MQHTPGPVPPPRLQTAAGRLILDGAPWLMRAAEFHYFRIAPERW
                     APGLARLAALGFNAVSTYVPWIWHEPAPGQRDFTGITDPRRDVLGFAHACRAAGLPLI
                     VRPGPFIYAEYQGLGHPRWLEQAIPEALMRGPRGKPVRGAFWAAYSLGHPAYRAAVAG
                     WYGAVAGAFAGLWNDPILAWQLDNETGFLYANGLGKWDWNPDTVARFHAWLRGEYGTV
                     ARLNAAWGARYPDFAAIGPPRVPFRRGLTQDYQRFLEAWIDDYLAWLRDTAQDAGVPV
                     PLSHNDSANFIPPINPARKLAAGVVELPGYDLYVKMTGARPATDYPWGSACASAYFRA
                     VTPTALPLLAWELGAGWFDPRARLTDAALLNNLVGALAQGLQGFTLYIAHDAVEVEGH
                     PYSYQTVLDAAGQPGPRYAIMQRLLAFLQAHEADLLAAAPPGPPRDSAGASPILGFAF
                     HYADFRFTPEDFLPLIGLQDPARILALVMSPFGIYAALLAAGYGPILRLINLETVRPA
                     DLAACTAVVMPNRGVLAPATLQMLEDYVQAGGHLITCGRTPRRTLTGAPLPSRRLYPA
                     PVQRTRFLGRLPALLYLGWEWLVKYQLFERPRLRRRHPTSMHIIDLGEPLYTLFYAPQ
                     FGSPLQRADGATVRGDYLRQTYRLPDGATPQLWTHGACAGYTGRNGAGSSTMIGSMPG
                     GSYFTAEYYRLDAATRRGIRAFWQETLAPFGLAPPVALSGDLEIEVTHRPFAGGGLLF
                     LLNRGPEPQRGTIRFGPAAGVRGAAQVIFAATTGQAVARGADALDVDLPGNEVLILRW
                     TEPGA"
     gene            3264..3827
                     /locus_tag="VF276_01835"
     CDS             3264..3827
                     /locus_tag="VF276_01835"
                     /inference="COORDINATES: protein motif:HMM:NF021577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DICT sensory domain-containing protein"
                     /protein_id="HEX6605630.1"
                     /translation="MDDVHDLIDRQTATWPDTAFREFTLDEARTLAGAPLTFTTSVLG
                     MEAISHVIEDHAAALGRRVEVFACFQKLSNALAQRARYEAILAAGSQVYLYGEPDWPA
                     WDHPHLRVVPIRPGHPLADVWLVALCDPRHVSMALLARELPDSAGRIRRQGDLIDRRF
                     QGFKTYDGNLINEMVEGLKLYAGAARR"
     gene            complement(3995..>4393)
                     /locus_tag="VF276_01840"
     CDS             complement(3995..>4393)
                     /locus_tag="VF276_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX6605631.1"
                     /translation="RLIINSVSCTSTFSQKAAVEAITGPTEEVDHMVEQFRKRRELVI
                     GGLNQIPGLSCVMPQGAFYAFPNITGVGMDSRAYADYLLTQADVAVLPGTAFGQFGEG
                     YIRISFANSMENLQEALNRIARANERLLQK"
BASE COUNT          621 a         1615 c         1494 g          663 t
ORIGIN      
        1 gccggcggct cgggcgccga ccggcagttg aaggtctacc ggcaaaccgg cgacatgcgg
       61 cgcgtggtgg agttcttgat ccacgagacc atgcgcggcg tgccctacgc ggaggagcta
      121 gggctgcccg cgaccgacat gacgccgatc gcgccggaga cctgacgccg ggcgggccta
      181 acccccctgg cccccttccc ggcgcgggaa ggggggaacg acgcagcatt ggagcagcgc
      241 gactcccctc cccacggtgg ggaggggcgg gggagaggtt gctcagcgcc cggccagggc
      301 agcctgcacc gcccgccgca ggtcgggcac gaacaccggc ttgatcagga agcccgacac
      361 gcccaatgct tcggcctgct ggcgcagcgc ctgggtcggg tagccggtga tcatgatcac
      421 cggcagggcc ggggccaggg cgcgcagatc gtgcagcagg gccagcccgt tgcggccctc
      481 caggcgataa tccaccagga tcaggtcgaa cggcccccgc tccagttccg cgagcgccgc
      541 ctcggcggaa cgcaccccca cgatggcgcg gttgggcatg gtatgggtca gcgcgtcgta
      601 cagtaaggcg aagaaatcgg cgtcgtcgtc cacgacgagg atgcgtgagg cggcatgtgg
      661 ttgggtcccg ttgtccatgt cccggctgct ccgttctggt agatcgagcg cgccgcccac
      721 ggggggcggc caggtagcat ccttaccgcc agcttacgcg gcggcatgaa catattacat
      781 gggcggggac aaaaatgcaa cacactcccg gtccagtccc gccgccgcgc ctgcaaacgg
      841 cggcgggccg gctcatcctc gacggcgccc cgtggctgat gcgggccgcc gagtttcatt
      901 actttcgcat cgcgcccgag cgctgggcgc cgggcctggc gcggctggcg gcgctcggct
      961 tcaacgccgt cagcacctat gtgccctgga tctggcacga accggcgccg gggcaacgcg
     1021 acttcaccgg gataaccgac ccccgccgcg atgtgctggg cttcgcccat gcctgccggg
     1081 ccgccgggct gccgctgatc gtgcgccccg gcccgttcat ctacgccgag taccagggcc
     1141 tcggccaccc gcgctggctc gaacaggcca tcccggaggc gctgatgcgc gggccgcgcg
     1201 gcaagccggt ccgcggcgcg ttctgggccg cctacagcct gggccacccg gcctaccggg
     1261 cggcagtggc cgggtggtat ggcgccgtag cgggggcctt cgccggcctc tggaacgacc
     1321 ccatcctggc ctggcaactg gataacgaga ccggcttcct gtacgccaac gggctgggca
     1381 aatgggactg gaaccccgac accgtggccc ggttccacgc ctggctgcgc ggcgagtatg
     1441 gtactgtcgc ccggctcaac gccgcctggg gcgcgcgcta cccggacttc gccgccatcg
     1501 gcccgccgcg tgtgcccttc cggcgcgggc tgactcagga ctaccagcgc ttcctcgaag
     1561 cctggatcga tgactacctc gcctggctgc gcgacacggc gcaagacgcg ggggtgccgg
     1621 tgcccctgag ccacaacgac agcgccaatt ttatcccgcc gatcaacccg gcgcgaaagc
     1681 tcgccgccgg ggtggtcgag ctaccgggct atgacctcta cgtgaagatg accggcgcgc
     1741 gcccggcgac cgactatccc tggggcagcg cctgcgcgtc ggcctacttc cgcgccgtca
     1801 cgccgaccgc gctgccgttg ctggcctggg aactgggggc cgggtggttc gacccgcgcg
     1861 cccgcctcac cgatgcggcc ttgctcaaca acctggtggg cgcgctggct cagggactgc
     1921 agggcttcac gctgtacatc gcgcacgacg ccgtcgaagt cgagggccac ccctacagct
     1981 atcagacggt gctcgacgcc gcggggcagc cagggccgcg ttacgcgatc atgcagcgcc
     2041 tgctggcgtt cctgcaagcc cacgaggccg acctgctggc cgccgcaccg cccggcccgc
     2101 cccgcgactc cgccggggca tcgcccatcc tcggctttgc cttccactac gccgactttc
     2161 gcttcacgcc ggaggatttc ttgccgctca tcggcctgca agacccggcg cgcatcctgg
     2221 ccctggtcat gagcccgttc ggcatctacg ccgcgttgct ggccgcgggc tacggcccga
     2281 tcttgcgcct gatcaacctg gagacggtcc gccccgccga cctcgccgcc tgcaccgccg
     2341 tcgtcatgcc caaccgcggc gtcctggccc cggccacgtt gcagatgctg gaagactatg
     2401 tgcaggcggg cggccacctg atcacctgcg gacgcacgcc gcgccgcacg ctgaccggcg
     2461 ccccgctgcc gtcgcgccgc ctctacccgg cgccggtcca gcgcacgcgc ttcctggggc
     2521 ggctgccggc cctgctctac ctgggctggg agtggctggt caagtaccaa ctgttcgagc
     2581 ggccccgcct gcgccgccgg cacccgacca gtatgcatat catcgacctc ggcgaaccgt
     2641 tgtacaccct gttctatgcg ccgcaattcg gcagcccctt gcagcgggcg gacggggcaa
     2701 cggtacgcgg tgactatctg cggcaaacgt accgcctgcc ggacggcgcc acgccgcaat
     2761 tgtggacaca cggggcctgc gccgggtata ctggccgcaa cggcgccggc agcagcacca
     2821 tgatcggcag tatgccgggc gggagttact tcacggcaga gtactaccgg ctcgacgccg
     2881 cgacccggcg aggcatccgc gccttctggc aggagacgct ggcgccgttc gggctggcgc
     2941 cgccggtggc gctcagtggc gacctggaga tcgaggtgac gcaccggccc tttgcgggcg
     3001 gcgggttgct ctttctcctc aaccgcgggc ccgagcccca gcgcggcacc atccgcttcg
     3061 gccccgccgc cggggtccgc ggcgcggcgc aggtgatctt cgccgccacg acggggcagg
     3121 ccgtcgcccg cggcgccgat gcgctcgacg tggacctgcc gggcaacgag gtgttgatct
     3181 tgcgctggac cgagccgggc gcttagtgac atggggccgc gccgctgccg cgcactgcca
     3241 tccggcccca ctggaggcga cgcatggatg atgtccacga cctgatcgac cggcagacgg
     3301 ccacctggcc cgacacggcc ttccgcgagt ttaccctcga cgaggcccgc accctggccg
     3361 gcgcgccgct caccttcacc acgtcggtgc tgggcatgga ggcgatcagc cacgtcatcg
     3421 aggaccacgc cgccgcgctg ggccgccggg tcgaggtctt cgcctgcttc cagaagctgt
     3481 cgaacgccct ggcccagcgc gcccgctacg aggcgatcct cgccgccggc agccaggtct
     3541 atctttacgg cgaacccgac tggcccgcct gggaccaccc ccacctgcgc gtcgtgccca
     3601 tccggcccgg ccacccgctg gccgatgtct ggctggtggc cctctgcgac ccccgccacg
     3661 tgtccatggc gctcctcgcc cgcgagttgc ccgacagcgc cgggcgcatc cgccgccagg
     3721 gcgacctgat cgaccggcgc ttccagggct tcaaaaccta tgacggcaat ctcatcaacg
     3781 agatggtgga ggggttgaag ctctacgccg gggccgcccg ccgctaaaca gacacgcccc
     3841 accggtggtc ccggtggggc gtgttcgcgg cggcgcgggc cggtttgaaa ccggccccta
     3901 caatgacgag tcgccgtctt tccccccttc ccgcggcggg aaggggggtt ggggggttag
     3961 gttggtcggg ggccgagatg tccccgcgtg gcgcctactt ctgcagcaac cgctcgttgg
     4021 cgcgcgcgat gcggttcagc gcctcttgca ggttctccat cgaattggcg aagctgatgc
     4081 ggatatagcc ctcgccgaac tggccgaacg ccgtgccggg cagcaccgcc acgtcggctt
     4141 gcgtcagcag gtagtcggcg taggcgcggc tgtccatgcc cacgccggtg atgttgggga
     4201 aggcgtagaa cgcgccctgc ggcatgacgc aactcaggcc ggggatctgg ttcagcccgc
     4261 cgatcaccaa ctcgcgccgc ttgcggaact gctcgaccat gtgatcgact tcttccgtcg
     4321 gcccggtgat cgcctcgacc gccgccttct ggctgaaggt ggacgtgcag gacacgctgt
     4381 tgatgatcag gcg
//
LOCUS       DASUYC010000115         4299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1224732, whole genome shotgun sequence.
ACCESSION   DASUYC010000115 DASUYC010000000
VERSION     DASUYC010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224732"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1302
                     /locus_tag="VF276_01845"
     CDS             <1..1302
                     /locus_tag="VF276_01845"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX6605632.1"
                     /translation="LGAPEILQPALVAGSDLGTQGTDWAASGLRVLLFARRAGGAPLH
                     DTQGRPQLPVDLVPLGLVSFSDELRPEAQATLREFAESGIRVKIISGDHPETVAALAR
                     QAGLVLEGDSAVVSGLDLAALDENQFAARAGAATIFGRITPQQKERLVRVLRAQGRYV
                     AMIGDGVNDVLALKQANLGIAMQSGSQATRSVADMVLLGDSFAALPAAFREGQRIVNG
                     MHDVMRLLLSRTVYVTLLIAATTMAGVAFPLTPKYTTVQGLLAVGIPTLALAAWARSG
                     PRRLSLRRALVHFVLPAGYTMAVLGLGLYLLYLLPAGDAGLARTVLTTSTLLCGLLVI
                     VFAEPPTRLWADGDTLSGDWRPTILAAALLALYVLIMLIPAWRGFFELQPLSLWDVLL
                     IVVLTAGWAVVLRFLWHRDVVERMLGVDVRPSAAGGPAPAS"
     gene            1491..1856
                     /locus_tag="VF276_01850"
     CDS             1491..1856
                     /locus_tag="VF276_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEX6605633.1"
                     /translation="MPDTAVYRQRLEAERARLLEDLHRFDEEVTDSADDLDPTRGGVG
                     NHMADDANVMFEEEKFVALRRNTEKMLEQVDRALERVAEGTYGICANCGKPIPPERLE
                     ARPSATLCVNCQQQLESAS"
     gene            1858..2481
                     /gene="lspA"
                     /locus_tag="VF276_01855"
     CDS             1858..2481
                     /gene="lspA"
                     /locus_tag="VF276_01855"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX6605634.1"
                     /translation="MEVRNPPVPSNAAGDPAPAAVDSGAAAAQPPMTLAARVRAVLPL
                     LALAALILIADQLTKLWIVAAIGRAAPVHEIDIIPGWFVFQYVENTGAAFGMFTNGSW
                     ILAFIAVAVVVGIVVMAPRMQQEATAGLPRWQIWLSLGLVLGGAVGNLADRLTRGYVV
                     DFVLVPKAQITLGNTLYHFPNFNVADSAITVGIILLLIGLLFAGERK"
     gene            2504..3448
                     /locus_tag="VF276_01860"
     CDS             2504..3448
                     /locus_tag="VF276_01860"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX6605635.1"
                     /translation="MPEFTAVVTPDQAGERLDRVLPLLGGDLSRSYGQQLIAAGQVLL
                     NGRPARASQKLKAGDTVQVTIPAAAPTAVEPEPMDLRVVYEEADVLVVDKPAGLVVHP
                     APGHARGTLVNALLAHTDQLSLHGDIRPGIVHRLDKDTSGLLVVARTDAAHADLVRQH
                     QARTMDKEYLGLVQGQPQPPTGLIDAPIARDPRDRKRQAVLATGRPARTRYTTETVYH
                     PGHNLETCALLRVRLETGRTHQIRVHMAYIGHPILGDAVYGARTLRAARALGLERQFL
                     HATALAFDLPSSGRRVAFTSPLPPDLAAVLARLGPPDS"
     gene            3469..>4299
                     /locus_tag="VF276_01865"
     CDS             3469..>4299
                     /locus_tag="VF276_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX6605636.1"
                     /translation="MRILITGSSGQIGTNLALRLVAEGHQVFGVDKRLNTWTDAFRYI
                     LQDLSGHYPAYPTGIGGVDYPPADVVVHLAAHAKVHQLVRYPHRALENMIMTFNVLEY
                     CRQTGTPIIFSSSREVYGDIHRFSTEETSADFAYTESTYSASKITGEALIYSYARCYG
                     LRYLVFRFSNVYGRYDNDIARMERVIPLFIRRIGRDEPITIYGPEKVLDFTYIDDCVD
                     GIVRGITALASGQVSNQTINLAYGEGNTLIRMAELVGAALGRTPRITVAPPLLGEVTH
                     Y"
BASE COUNT          642 a         1526 c         1409 g          722 t
ORIGIN      
        1 ctgggagcac cggagatcct ccagccggcc ctcgtagctg ggagcgacct gggcacgcag
       61 ggtactgact gggcggcaag cgggctgcgg gtgctgctgt tcgcgcggcg ggcgggtggc
      121 gcgccgctac acgataccca gggccggccc cagcttccgg tggatctggt gccgctgggc
      181 ctggtgagtt ttagcgatga attgcgcccg gaggcccagg cgactctgcg cgagtttgcc
      241 gagtccggta tccgggtcaa gatcatctcc ggcgaccacc ccgaaacggt cgccgcgctc
      301 gcgcgccaag ccgggctggt cctggagggc gatagcgccg ttgtctcggg gctagacctg
      361 gccgcgctgg acgagaacca gtttgccgcc cgggcggggg cggccacgat ctttgggcgc
      421 atcacgccgc agcaaaaaga gcgcctggtg cgggtcttgc gcgcgcaggg ccgctatgtc
      481 gccatgatcg gcgatggggt gaacgacgtg ctggcgctga agcaggccaa cctgggcatc
      541 gccatgcaaa gcggcagcca ggccacgcgc agcgtcgccg atatggtgtt gctgggcgac
      601 tcgtttgcgg cgttgcccgc cgcgttccgc gagggccagc gcatcgtcaa cggcatgcac
      661 gacgtcatgc gcctgctgct ctcgcgcacc gtctacgtga ccttgctgat cgccgccacg
      721 acgatggccg gcgtcgcctt cccgctcacc cccaagtaca ccacagtgca gggcctgctg
      781 gccgtcggca ttcccaccct ggccctggcc gcatgggctc gctccggtcc gcggcgcctg
      841 agcttgcgcc gggcgctcgt gcacttcgta ctgcccgccg gctacacgat ggcggtgctg
      901 ggcttggggt tgtatctgct ctacctgctg ccggcgggcg atgccggcct ggcccgcacg
      961 gtgctgacca ccagcaccct gctgtgcggg ctcctcgtga tcgtgttcgc cgaaccgccc
     1021 acccgcctgt gggccgatgg cgataccttg agcggcgact ggcgccccac gattctagct
     1081 gcagcgctgc tggcgctcta tgtgctgatc atgctcatcc ccgcctggcg cgggtttttc
     1141 gagttgcagc cgctgagcct ctgggacgtt ttactgatcg tcgtgctgac ggccgggtgg
     1201 gccgtggtgt tgcgctttct gtggcaccgc gacgtggtgg agcggatgct gggcgtcgat
     1261 gtccggccgt ccgcggccgg cggcccggct cctgcttctt gatctctcgc ccggtggcgc
     1321 atacctgctt gcatggatgg cggcaccggg tgcctgccag gaccggtcgc gcgacacgct
     1381 ctcgccgctg aggccggttg ctcagaccgg aagctttgtg ctataatctg aagctaacgc
     1441 agccgcaccc ggatgcacgc cgcatgttat tggtgccgga ggagaattaa atgcctgata
     1501 ccgccgttta tcggcagcgc ctcgaagccg agcgcgcgcg cctgctggaa gatctccacc
     1561 gcttcgacga ggaagtgacg gacagcgcgg acgacctgga ccccacgcgc gggggcgtgg
     1621 gcaaccacat ggccgacgac gccaacgtca tgttcgagga agagaagttt gtcgccctcc
     1681 gccgcaatac cgagaagatg ctggaacagg tggaccgggc gctggagcgc gtcgccgagg
     1741 gcacctacgg catctgcgcc aattgcggca agccgatccc gcccgagcgg ctggaggcgc
     1801 gcccgtcggc cacgctctgc gtcaactgcc agcagcagct agaaagcgca tcgtaggttg
     1861 gaggttcgca atccgccggt tccgtcgaat gccgccggcg acccggctcc ggcggcggtc
     1921 gattccggag cggcggcggc gcaaccgccg atgacgctgg ccgcgcgcgt gcgcgcggtg
     1981 ctgcccttgc tggccctggc cgcgctgatc ttgatcgccg accagttgac caagctctgg
     2041 atcgtggcgg cgattggccg cgccgcgccg gtgcacgaga tcgatatcat ccccggctgg
     2101 tttgtgttcc agtatgtcga gaacaccggt gccgcgttcg gcatgttcac caacgggagc
     2161 tggatactgg cgtttattgc ggtggccgtg gtggtcggca tcgtcgtcat ggcgccgcgc
     2221 atgcagcaag aggcgaccgc aggcctgccg cgctggcaga tctggctcag cctgggactg
     2281 gtgctcggcg gggcggtggg caacctggcc gaccggctca cccgcggcta cgtggtcgac
     2341 ttcgtgctgg tgcccaaggc gcagatcacg ctgggcaaca cgctctatca cttccccaac
     2401 tttaacgtgg ccgacagcgc cattaccgtc ggcatcatcc tgctgctgat cggcctgttg
     2461 ttcgccgggg agcgcaagta agcccccggc gcggccccct gccatgcccg agttcaccgc
     2521 cgtcgtcacc ccggatcagg ccggcgagcg cctggaccgg gtgctgcccc tgctcggcgg
     2581 cgacctctcg cgctcttacg gccagcagtt gatcgccgcg ggccaggtgt tgctgaacgg
     2641 ccgcccggcg cgcgcctccc agaagctgaa ggccggcgac accgtgcagg tcacgatccc
     2701 cgccgccgcg cccaccgccg tcgaacccga accaatggac ctgcgcgtgg tttacgagga
     2761 agccgacgtg ctggtggtgg ataaaccggc ggggctggtc gtccaccccg cgcccggcca
     2821 cgcccgcggc acgctcgtca acgcgctgct ggctcacacc gaccagctct cgctgcacgg
     2881 cgatatccgc cccggcatcg tgcaccggct cgataaggac accagcggct tgctggtcgt
     2941 ggcccgcacc gacgccgcgc acgccgatct cgtccgccag caccaggcgc gcaccatgga
     3001 caaggagtat ctggggctgg ttcagggcca accccagccg cccaccggcc tgattgacgc
     3061 gcccatcgcc cgcgacccgc gcgaccgcaa gcgccaggcc gtgctggcga ccgggcgccc
     3121 ggcccgcacg cgctacacca ccgagacggt ctatcatccc ggccataacc tggagacctg
     3181 cgcgctgctg cgggtgcgcc tcgaaacggg ccgcacacac cagatccgcg tccacatggc
     3241 ctatatcggc cacccgattc tcggcgatgc ggtctacggc gcacggaccc tgcgcgcggc
     3301 gcgcgccctg ggcctggaac gccagttctt gcacgccact gccctggcct tcgacctgcc
     3361 gtcgtcgggg cggcgcgtgg ccttcaccag ccccttgccg cccgacctgg ccgccgtcct
     3421 ggcccggttg gggccgcccg attcctgact cagccccaga ggagccccat gcgtatcttg
     3481 atcaccggtt ccagcggcca gatcggcacc aacctggcct tgcgcctcgt ggccgagggc
     3541 caccaggtct tcggcgtcga taagcgcctc aacacctgga ccgacgcctt ccgctacatc
     3601 ttgcaggatc tcagcggcca ttatcccgcc tatcccaccg gcatcggcgg tgtcgattac
     3661 ccacctgccg atgtggtcgt ccacctggcc gcccacgcca aggtgcatca gttggtgcgc
     3721 tacccgcacc gggcgctcga aaacatgatc atgaccttca atgtgctcga atattgccgc
     3781 cagacgggca cgccgatcat cttttccagc agccgcgagg tctacggcga catccatcgc
     3841 ttcagcaccg aggagacgag cgccgacttc gcctacaccg agagcaccta ctcggccagc
     3901 aagatcaccg gcgaggccct gatctactcg tatgcgcgct gctacgggct gcgctacctg
     3961 gtcttccgct tcagcaacgt ctatggccgc tacgacaacg acatcgcgcg tatggagcgg
     4021 gtgatcccgc tcttcatccg ccgcatcggc cgcgacgagc cgatcaccat ctacgggccg
     4081 gagaaagtgc tcgattttac ctacattgac gactgcgtgg acggcatcgt gcgcggcatc
     4141 accgcgctgg cgagcgggca ggtcagcaac cagacgatca acctggccta cggcgagggc
     4201 aacacgctga tccgcatggc cgaactggtg ggcgccgccc ttggccgcac cccgcgcatc
     4261 actgtcgcgc cgccgctgct cggcgaggtc acgcattac
//
LOCUS       DASUYC010000116         6870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1470719, whole genome shotgun sequence.
ACCESSION   DASUYC010000116 DASUYC010000000
VERSION     DASUYC010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6870
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1470719"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..528)
                     /locus_tag="VF276_01870"
     CDS             complement(88..528)
                     /locus_tag="VF276_01870"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX6605637.1"
                     /translation="MSTEENKAVVRAYIEEVTNKGDIAAVPQYVTGDFREHYGPPGLP
                     QGIAGAIALVQIFKDNFDGYRFEIEELLAEGDRVLVRGWGSGTHTGPFLGIPPTGKAI
                     RFRAAHVFQVVDGKLAARWAYPDMLGMLQQLGAAPMPGAPPPGR"
     gene            complement(715..2430)
                     /locus_tag="VF276_01875"
     CDS             complement(715..2430)
                     /locus_tag="VF276_01875"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX6605638.1"
                     /translation="MEDLKLVLDLVLAVAAAFAGGAAAQRLGQPVILGYLIAGMIIGP
                     FTPGPVADQHSVSVLAEIGVAFLMFALGAEFSLGELRRMGRVASVGGVLQILITMGLG
                     PLLAPILGLTVVQGIFLGALLALSSTVVALKLLMARGELQALHGRVALGILIAQDLAV
                     VPMVVILPAVASGSATLAQDLGLAALKAAGILAGAYVLGTRLVPALLRQIAVARTREL
                     FLLGVVALALGTALVTQAAGLSIAFGAFLAGLVVSESEFRTQVIAEVLPLRDLFASLF
                     FVSVGMLINPVTLLDQLGWVALVAAVVLVGKTVIVTAIVLALKLPGRVALLSGLALAQ
                     VGEFSFVLARIGVDRDAIPPIIFDLILAVSLVTIVLTPALLGLAPGLLRRLERLPGLG
                     RRFTAPVEGATETEDLAQHVVICGFGRVGRELAEALEQRGLRYLVIEYNPLIVDELRR
                     RQVPVIYGDAANPAVLEHAHLDTARVMAVLMPDARAAELAVHHTRLDYPDLDIIARVG
                     NARQVDQLRRVGASEVVQPEFEAGVEVLLQVLRHYDMNERELIQMVSGRRTAFYQRPL
                     MDELA"
     gene            complement(3021..6080)
                     /locus_tag="VF276_01880"
     CDS             complement(3021..6080)
                     /locus_tag="VF276_01880"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HEX6605639.1"
                     /translation="MGRTYIALDTEATGLRAEADEIIEIGAVKFRDGQVLDHWQSFIR
                     PSQPIPYKITALTGITPQDVRRAPALYAVAPALLRFVGDCPIVGHSIEIDLALLKRQG
                     VQMRNRSYDTFELATLLLPEVPVYNLAGVAAALQLPIPTTHRAVADAELAMHVFNALV
                     GRLQELPVEVLAEINRATATTEWSLRYLFQEVEREAVRSTFSTPLSAAGSIGSKLAAA
                     GFSQTEFDLGLIHPPETPPPLDPDDDTTRPIDIPALLDALGADGVVAQTFPAYEFRPQ
                     QLDMLRSVAEAFNNGDHLLVEAGTGTGKSLAYLLPALKFAVQNNTRVVVATNTINLQD
                     QLFHKDLPALTEVLAAWDAQTRRPAADDAWSQALARQSGRAGRKGQHDHKAAGSATPQ
                     EPPFRAALLKGRSNYLCLRRWAGFRKQTPTNIDELRTLVKVLIWLPTTHTGDRSELLL
                     VNTEPQAWNKIAVSEEGCPLHECVYNQKGLCFFHRARVNAEAAHVLVVNHALLLADIA
                     TGNRVLPPYKHLVIDEAHHLEDEATDQLGYQVTQHAVLELTTELSHRHGDHYEGFLAD
                     LRRQFDAAPATRPAASLRGTLPPLERVHVSSRELQALDQILEQLHAACERARPMAHEV
                     FTCMHRLLEAHSEERNEYDKRLRLTPDVRKGPLWGEVELTWDNLNLVLAGLLDDLNRL
                     ITVIEDLDAAQSGDLANLLLELSNLWRQVETLHTRMKAAIANPSAGAIYWLSSNARDE
                     TTTLHCAPLHVGELLDRYLFQTKRTVVLTSATLSTDGDFGYVRERLGLHTTGELHVGS
                     PFDYVNSTLLYIPEDLPEPNQPGYQRQLEQTVHDLCKATRGRALVLFTSHSALRTTYR
                     ALQRPLEESGLLLLGHNLDGSRRQLLERFRNTPHSVLMGTSSFWEGIDVVGDALSVLI
                     IAKLPFRVPSDPVFAARSEQFDDAFAQYSVPQAILKFKQGFGRLIRSREDRGVVAVLD
                     RRVISKGYGHTFLTSLPVCTVRRGPAHNMPDEAARWLAPAPAPVAEE"
     gene            complement(6133..>6870)
                     /locus_tag="VF276_01885"
     CDS             complement(6133..>6870)
                     /locus_tag="VF276_01885"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEX6605640.1"
                     /translation="DHMIGAGMFDRRPDVLVTTASGIHAIPISEQVIGYILTFCRGFL
                     RDWRAQREARWDRYTPEEAAGKTLGIVGYGPIAQRTAALAHALGMHVLVLRHTPHAQA
                     EPPIERFYTPDQLHEMLGASDFVLLAAPLTPETRGMIDAAALAAMRPTAVFMNISRGG
                     LVDEPALIAALQGGKIAGAALDVFAQEPLPGESPLWAMQNVFITPHMAGANPSYNHRA
                     TELFCDNLRRYIAGEPLRNQVDPARGY"
BASE COUNT         1151 a         2292 c         2363 g         1064 t
ORIGIN      
        1 atgctgaccg gcgccacatg agccggcccc gccctggcta tctctgcagg tgacaatggg
       61 cggaggccgg aaaccgtgtc acatcgccta ccggcccggc gggggcgcgc cgggcatggg
      121 ggccgcccca agctgctgca acatgccgag catatcggga taggcccagc gcgccgcgag
      181 tttgccgtcc accacctgga agacgtgcgc cgcgcggaag cggatggctt tgccggtggg
      241 cgggatgccc aggaaggggc cggtgtgcgt gccgctgccc catccgcgca ccagcacccg
      301 gtcgccttcg gccagcagct cctcgatctc gaagcgatag ccgtcgaagt tgtccttgaa
      361 tatctggacc agcgcaatgg ctccggcgat gccctgcggc aaccctggcg gcccgtaatg
      421 ctcccggaaa tccccggtga cgtattgggg caccgcggcg atatcgccct tattcgttac
      481 ctcctcgatg tatgcgcgca caacggcttt gttctcctcg gtggacacag cacaaccctc
      541 cctatcgagc gtagcaagca acgtatggcc ggaccgcgca gggacacgtc gcccgcgcgc
      601 cgcaactacc ggcgcggcta cccagactct agcgcgccgg gttgcttata tgccagccca
      661 aacgatgctc gttttttgct caatcgaata ctgccgtagg cggaggccac gcgatcaggc
      721 cagttcgtcc atcagggggc gctggtagaa cgccgtgcgc cgcccgctga ccatctggat
      781 cagttcccgc tcgttcatgt cgtagtggcg cagcacctgc aagagcactt ccacccccgc
      841 ctcgaactcc ggttgcacca cctcgctggc gcccacgcgg cgcaactgat cgacctgccg
      901 ggcgttgccc acccgcgcga tgatgtcgag atccgggtag tccaggcgcg tgtggtggac
      961 ggccagttcg gcggcccgcg catcgggcat gagcacggcc atgactcgcg cggtgtccag
     1021 gtgggcgtgc tccagcacgg cggggttcgc ggcgtcgccg tagatcaccg gcacctggcg
     1081 ccgccgcaat tcatccacga tcagcgggtt gtattcgatg accaggtagc gcaagccgcg
     1141 ctgctctaac gcctccgcca gttcgcggcc tacccggcca aacccgcaga tgacgacgtg
     1201 ctgcgccagg tcctccgtct ccgtcgcccc ttccaccggc gcagtaaacc ggcgccccag
     1261 gccgggcagg cgctccaacc ggcgcaataa gccgggcgcc aacccgagca gcgcgggcgt
     1321 cagcacaatc gtcaccagcg agacggccag gatgaggtcg aagatgatcg gggggatggc
     1381 gtcgcggtcc acgccgatgc gggccagcac gaaggagaac tcgcccacct gggccagcgc
     1441 cagcccgctg agcaacgcga cccgccccgg caacttcagc gcgagcacga tcgcggtgac
     1501 aatcaccgtc ttgcccacca ggaccacggc ggccaccagg gccacccatc caagctggtc
     1561 cagcagcgtc accgggttaa tcagcatgcc gaccgagacg aagaacagcg acgcaaacag
     1621 gtcccgcaac ggcaacacct cggcgatcac ctgggtgcgg aactccgatt cggagaccac
     1681 caggccggcc aggaaggcgc cgaaggcaat cgacagcccg gcggcctgcg tcaccagcgc
     1741 cgtgcccagc gccagcgcga cgacgcccag caagaacaac tcgcgcgtgc gggcgacggc
     1801 gatctggcgc agcagcgcgg gcaccaggcg cgtgccgagc acgtaggcac cggccaggat
     1861 gcccgccgcc ttgagcgccg ccaagcccag gtcttgcgcc agcgtcgcgc tgccgctcgc
     1921 caccgcaggc aagatcacca ccatcggcac cacggccagg tcctgggcga tcaggatgcc
     1981 cagcgccacc cgcccatgca gcgcctgcaa ctccccgcgc gccatgagca gcttgagcgc
     2041 gacgaccgtg ctcgacagcg ccagcagcgc gcccaggaag atcccctgca cgaccgtcag
     2101 ccccaggatg ggcgccagca gcgggcccag gcccatagta atcaggatct gcaacacgcc
     2161 gccgacggaa gccacccggc ccatgcgccg cagttcgccc agcgagaact cagcacccag
     2221 cgcgaacatg aggaaggcca ccccgatctc ggccagcaca ctcaccgaat gttgatcggc
     2281 aacgggaccg ggtgtgaagg ggccgatgat catccccgcg atcagatagc cgagaatgac
     2341 gggctggccc aggcgttggg ccgcagcccc gccggcaaag gctgccgcca cggccagtac
     2401 caggtctaga acaagtttca gatcttccat agcgattact ccagccatca agcgtgtggg
     2461 ccataaacct gcggcactac ggtgatgggg ggcggccgaa ccggtgaggc ggcgccctgg
     2521 gttcgcgata aattggaggc taccggacag ccagcgcgcg tggggccccc gcggcaggtc
     2581 cgatcggttg gatggatggc aaaatagcag gtgatgcgtg gccggcggat gggtcatgcc
     2641 ggatggtacg gattcgcgct gaggccgtgt gccgcgcggc ggggccggcg ggagtagggc
     2701 gcccgccgaa agcgacgcgg atcagattaa tggcacggag gcggataagc agcgccggca
     2761 ggcgggccgg gtcgtggacc ggtggtaccg ctacgccctg ataggggccg ggatgggcgg
     2821 accgggatgg caggggtgat cggcggggtc gcgcgggggg tccatccatg ggcatggttc
     2881 ctcctgggcc ggtcgtctct gaattagcaa tacgcgatag cagtgccttc cattgacaac
     2941 gggagatgcc gggggccggt gcttgccggc taaactcgaa cctaattcta tactatcggc
     3001 ccgtggcggg caaggcggcg ttactcttcg gcgacgggcg cgggggccgg ggcgagccag
     3061 cgcgcggcct cgtccggcat gttgtgtgcc gggccccgcc gcaccgtgca gacgggcaac
     3121 gaggtgagga aggtgtgccc gtagcccttg ctgatcaccc ggcggtcgag cacggccacc
     3181 acgccgcggt cctcccggct gcggatcagt cggccgaagc cctgcttgaa cttcaggatc
     3241 gcctgcggca ccgagtactg ggcaaaggcg tcgtcgaact gctcgctgcg cgcggcaaag
     3301 accgggtcgc tgggcacgcg gaagggcaac ttagcgatga tcagcacgct cagcgcgtcg
     3361 cccaccacgt cgatgccctc ccagaaggac gaggtaccca tcagcacgct gtgcggggtg
     3421 ttgcggaagc gttcgagcaa ctggcggcgc gaaccgtcca ggttatggcc cagcagcaac
     3481 agccccgatt cttccagcgg gcgctgcaag gcgcggtacg tcgtgcgcag ggcgctgtgc
     3541 gaggtgaaca gcaccagcgc ccggccccgc gtcgccttgc acaggtcgtg gacggtctgc
     3601 tccaactgcc gctggtagcc cggctgattc ggttcgggca gatcttcggg gatgtagagc
     3661 agcgtggagt tgacataatc gaacggtgag ccgacgtgca actcgccggt ggtatgcagg
     3721 cccagccgtt cgcgcacgta gccgaagtcg ccgtccgtgg agagcgtagc cgaggtcagc
     3781 accaccgtgc gcttggtctg gaacaggtag cggtcgagca attcgccgac atgcagcggc
     3841 gcgcaatgca gggtagtggt ctcgtcgcgg gcgttggacg acagccagta gatagcgccc
     3901 gcgctggggt tggcgatggc cgctttcatg cgcgtgtgca gcgtttccac ctggcgccac
     3961 aggttggaca gctccagcag cagattcgcc aggtcgccgc tctgcgccgc gtccaggtct
     4021 tcgatgacgg tgatcagccg gttcaggtcg tccagcaggc ccgccagcac caggttcagg
     4081 ttgtcccagg tcagttcgac ctcgccccag agcggcccct tgcgcacgtc gggcgtgagg
     4141 cgcaggcgct tgtcgtattc attgcgctcc tcgctgtgcg cctccagcag ccggtgcatg
     4201 caggtgaaca cctcgtgggc catgggccgc gcccgttcgc acgccgcgtg caactgctcc
     4261 aggatctggt ccagggcctg caactcgcgc gagctgacat ggacgcgctc aagcggcggc
     4321 agcgtcccgc gcagcgacgc agccgggcgc gtcgccgggg cggcgtcgaa ctggcggcgc
     4381 aggtcggcca ggaagccctc gtaatgatcg ccgtggcggt ggctcaactc ggtggtcagc
     4441 tccagcaccg cgtgctgcgt cacctggtag ccgagctggt cggtggcctc gtcctccagg
     4501 tggtgggctt cgtcgatgac caggtgcttg tagggcggca gcacgcgatt gccggtggcg
     4561 atgtcggcca gcagcagcgc gtggttgacg acgagcacgt gggccgcctc ggcgttcacc
     4621 ctggcgcggt gaaagaagca gaggcccttc tggttataga cgcactcgtg cagcgggcag
     4681 ccttcctcgc tgacggcgat cttgttccag gcctgcggct ccgtgttcac cagcaacaac
     4741 tcgctgcggt ccccggtgtg ggtcgtcggc aaccagatca gcactttgac cagggtgcgt
     4801 aactcgtcga tattggtggg cgtctgcttg cggaagccgg cccagcggcg caggcagaga
     4861 tagttgctac ggcccttgag cagagcggcc cggaacggcg gctcctgggg cgtggcgctg
     4921 ccggcggcct tgtggtcgtg ctggcccttg cggcccgccc gcccgctctg ccgggccagg
     4981 gcctggctcc aggcgtcgtc cgcggcgggc cggcgcgtct gggcgtccca cgccgccagc
     5041 acctcggtca gcgcgggcag atccttgtgg aacaactggt cttgcaggtt gatcgtgttg
     5101 gttgccacca ccacgcgggt attgttctgc acggcgaact tgagcgcggg cagcaggtag
     5161 gccagcgatt tgccggtgcc ggtcccggct tccaccagca gatgatcacc gttgttgaat
     5221 gcctcggcca ccgagcgcag catatcgagt tgctggggcc ggaactcata ggccgggaag
     5281 gtctgcgcca ccacgccatc ggcgcccagc gcgtcgagca gcgcaggaat gtcgatcggg
     5341 cgcgtcgtgt cgtcgtcggg gtccagcggc gggggcgtct ccggcgggtg gatcaggccc
     5401 aggtcgaact cggtctgcga aaacccggcg gcggccagct tgctgccgat gctgcccgcc
     5461 gcactcagcg gcgtggagaa ggtcgagcgg acggcctcgc gctccacttc ctggaacaaa
     5521 tagcgcagcg accactcggt ggtcgccgtc gcgcggttga tctcggccag cacttcgaca
     5581 ggcagctctt gcagccgccc caccagcgca ttgaacacgt gcatcgccag ttcggcgtcg
     5641 gccacggccc ggtgcgtcgt cgggatgggc aactgcaacg cggcggctac cccggccagg
     5701 ttatagaccg gcacttcggg cagcagcagc gtcgccagtt caaacgtgtc gtaggaccgg
     5761 ttgcgcatct gcacgccctg gcgtttgagg agggccaggt cgatctcgat ggaatggccg
     5821 acaatggggc aatcgccgac gaaccgcaac agcgccgggg ccacggcata taacgcgggc
     5881 gcccgccgca catcctgggg cgtgatgccg gtcagggcgg tgatcttgta ggggatgggc
     5941 tggctgggcc ggatgaagct ctgccagtga tccagcacct ggccgtcgcg gaacttgacc
     6001 gcgccgattt cgatgatctc atcggcttcg gcccgcaggc ccgtggcctc ggtatctaat
     6061 gcgatatagg tacgacccac ggtatgcgtt gttccgatct agacgacgac ccgctggacg
     6121 ggtccagcta catcaatacc cccgtgcggg gtccacctgg ttacgcaacg gctccccggc
     6181 gatgtagcgc cggaggttgt cacagaatag ctcggttgcg cggtggttgt aggatggatt
     6241 ggccccggcc atatgcgggg tgatgaacac gttttgcatg gcccacagcg gactctcgcc
     6301 gggaaggggt tcctgcgcga acacgtccag cgcggccccg gcgatcttac ccccttggag
     6361 cgcggcgatc agcgccggct cgtcgaccag cccgccgcgc gagatgttca tgaacacagc
     6421 ggtggggcgc atagcggcca gcgcggcggc gtcgatcatg ccgcgcgtct ccggggtgag
     6481 cggcgcggcc agcagcacaa aatcactcgc tcccagcatc tcgtgcaact ggtcgggggt
     6541 gtagaagcgc tcaatcggcg gctcggcctg ggcgtgcggt gtgtgccgca gcacgagaac
     6601 gtgcatgccc agcgcgtggg ccagggcggc ggtgcgctgg gcgatggggc catagccgac
     6661 gatacccagc gtcttgcccg ccgcttcctc cggcgtgtag cggtcccagc gcgcctcgcg
     6721 ttgcgcccgc cagtcgcgca aaaagccccg gcagaaggtg agaatatagc cgatgacttg
     6781 ctcggagatg gggatggcgt ggatgccgct ggccgtcgtc accagcacat ccggccgccg
     6841 gtcgaacatc ccggcgccga tcatgtggtc
//
LOCUS       DASUYC010000117         2864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_993542,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000117 DASUYC010000000
VERSION     DASUYC010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993542"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            360..1124
                     /locus_tag="VF276_01890"
     CDS             360..1124
                     /locus_tag="VF276_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX6605641.1"
                     /translation="MEQLLDPDVWVALITLTALEIVLGIDNVIFISILAGKLPADRQA
                     RARQLGLGLAMITRLLLLLSLSWIIQLTAPLFTVVGQEISGRDLVLIGGGLFLLAKST
                     HEIHQNLEGEGAHAEGRQPRATSFGSVIIQIGLLDIVFSLDSVITAVGMANQLWVMMT
                     AVVIAVGVMLVASGPISHFVDQHPTVKMLALSFLILIGVSLIAEGFDQHIPKGYIYFA
                     MGFSVFVELLNLRARGRAAKPVELRGPHLEEPAAQQ"
     gene            complement(1127..2503)
                     /locus_tag="VF276_01895"
     CDS             complement(1127..2503)
                     /locus_tag="VF276_01895"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019586973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX6605642.1"
                     /translation="MTTDAAREQQLGEQVAAAMEKYAVPGVALGIVQGDAEYVAGFGV
                     TNTEQPLPVDADTLFQIGSTTKTVTGTLAMRLVEAGTLDLDAPIRTYLPDLRLRDADV
                     AARVTMRHLLTHTGGWVGDYFDDTGRGEDAVTRIVDRVAELPQLTPLGEVWSYNNAGF
                     YLAGRVLEVLTGRPYETLVQEWVFAPLGMNRSFFFAEDMITYRVAAGHEEPHGEGATR
                     PTVARPWTVPRAAAPAGGIVSTVRDQLRYARFHLGDGTAPDGTRLLSPDSMALMQTAQ
                     VAANNDQQMGITWFLRDVEGTRIVAHGGATNGQESAFLLIPSRRFAMTVLTNSSRGSD
                     LNRDVTRWALEHYLGLHDRDPEPLAATADELTAYTGHYSAAAADVDLTMQGTDLMLQL
                     TPKGGFPTPESPAEPAPPPVRAALCGPDAILVLDEPLQGLRGEFLRGPDGQIAWLRAG
                     GRVMRRER"
     gene            complement(2671..>2864)
                     /locus_tag="VF276_01900"
     CDS             complement(2671..>2864)
                     /locus_tag="VF276_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605643.1"
                     /translation="SVKTVVLPFDKFPALPRVLGPEMQSTGEAMGVDTTYAGALAKAR
                     LAAGRSIAAPAPEAEAEAA"
BASE COUNT          406 a          949 c         1011 g          498 t
ORIGIN      
        1 ccggcggcca actcgtggcc gaaggcacgc cggaggaggt ggcccgtgtg tccgcctcgg
       61 ccacgggcgc gttcctccgc ccgctgctgt cgtaataccg cgggcgccgg cgggcgccgg
      121 tggggtatcg gcctcaccgg cgcccgcttg ccctttcgcc tgccctccat ccctctgccg
      181 gtcctcctgg ccttcctctc gccgctcgcc gcctgagagt ttgctgaggc tatcgccgct
      241 ggtatgctgt atgcttgctc tatacggccc ctttggggta tcgtataacc ctggtcccgg
      301 tgcgcggggg tcggggagtc tgccgcgccg tatagcaggc accggaagga gtatgattgg
      361 tggagcaact attggatccc gatgtctggg tagcattgat cacgttgacg gcgctcgaaa
      421 tcgtcctggg catcgacaac gtgatcttca tctcgatcct ggccggcaag ctgcccgccg
      481 accggcaggc gcgggcgcgg cagttgggtc tgggcctggc gatgatcacg cggctgctgc
      541 tgctgctctc gctgtcctgg atcatccagc ttacagcgcc gctgttcacg gtggtcgggc
      601 aggagatctc cgggcgcgac ctggtgctga tcggcggcgg cctcttcctg ctcgccaaga
      661 gcacgcatga gatccaccag aacctggagg gcgagggcgc ccacgccgag ggccggcagc
      721 cgcgcgccac gtcgttcggc tccgtgatca tccagatcgg gctgctggac atcgtcttct
      781 cgctcgactc ggtcatcacg gcggtgggca tggcgaacca gctctgggtt atgatgaccg
      841 cggtggtcat cgccgtgggc gtcatgctgg tggcctccgg cccgattagc cacttcgtgg
      901 atcagcaccc gacggtcaag atgctggctt tgagcttcct gatactgatc ggcgtctcgc
      961 tgatcgccga aggcttcgac cagcatatcc cgaagggcta catctacttc gccatgggct
     1021 tctcggtctt cgtggagctg ctgaacctgc gcgcccgcgg ccgggccgcc aagccggtag
     1081 agctacgcgg gccgcacctg gaggagccgg cagcccagca gtagacctac cgttcgcggc
     1141 gcatcacgcg gcccccggca cgcaaccagg cgatctggcc gtccggcccg cgcaggaatt
     1201 caccgcgcaa gccctgtagc ggctcgtcga ggaccaggat ggcatccggc ccgcagaggg
     1261 cggcgcgcac aggcggcggg gccggttcgg cgggcgactc gggtgtgggg aagccgccct
     1321 tcggcgtcag ttgcagcatc agatccgtgc cctgcatcgt cagatcgaca tcggcagcag
     1381 ctgcgctgta gtggccggta taggccgtca gttcgtcggc ggtggcggcc agcggttcgg
     1441 gatcgcggtc gtgcaggccg aggtagtgtt cgagcgccca gcgggtcacg tcgcggttga
     1501 ggtcgctgcc ccggctggaa ttggtgagca cggtcatggc gaagcggcgg ctggggatca
     1561 gcaggaacgc cgactcctgc ccgttggtcg cgccgccgtg ggccacgatg cgcgtgcctt
     1621 ccacatcgcg cagaaaccag gtgatgccca tctgctggtc gttgttggcc gcgacctgcg
     1681 cggtttgcat cagggccatg ctgtcgggcg agagcaggcg cgtgccgtcc ggggccgtgc
     1741 cgtcgcccag gtggaagcgc gcgtagcgca actggtcgcg cacggtggag acgatgcccc
     1801 cggcaggggc ggcggcgcgc ggcaccgtcc agggccgggc caccgtgggg cgggtagcac
     1861 cctcgccgtg cggctcctcg tgcccggcgg ccacgcgata ggtgatcatg tcctcggcga
     1921 agaagaacga ccggttcatg cccagcgggg cgaagaccca ttcctgcacc agcgtttcat
     1981 agggccgccc ggtgagcact tcgagcacgc gcccggccag gtagaacccg gcgttgttgt
     2041 aggaccagac ctcgcccagc ggggtcagtt gcggtagctc ggccacgcga tccacgatgc
     2101 gggtcacggc atcctcgccg cgcccggtgt cgtcgaagta gtcgcccacc cagccgccgg
     2161 tgtgcgtcag caggtggcgc atggtcacgc gggcggccac gtccgcatcc cgcaggcgca
     2221 ggtcgggcag gtaggtgcgg atcggggcgt cgagatccag cgtgcccgct tccaccaggc
     2281 gcatggcgag ggtgccggtg acggtcttgg tcgtcgagcc gatctggaac agcgtatcgg
     2341 cgtccaccgg cagcggctgc tcggtattgg tcacgccgaa gccggccacg tactccgcgt
     2401 cgccctggac gatgccgagc gcgacgccgg gcacggcgta tttctccatg gcagcggcga
     2461 cctgctcgcc cagttgttgc tcccgcgcgg cgtcggtggt cataatctgc tccttcgcat
     2521 tggggtctat gaagtgcggc cattatagca cagccggcgg gtacggacga cgcgccgcat
     2581 agttcgaggc gtcatatcgc gcatatttaa cccaagaaac cgccccttcc gtactacgaa
     2641 aggggcggca gacttagcag tgtagcgccg tcaggcagcc tcggcttcgg cctccggcgc
     2701 cggggcggcg atgctccggc ccgcggcgag gcgggctttc gccagggcac ccgcgtaggt
     2761 ggtgtccacg cccatggcct cgccggtgct ctgcatttcg gggccgagca cgcgcggcag
     2821 ggccgggaac ttgtcgaagg gcagcaccac cgttttgacc gaga
//
LOCUS       DASUYC010000118         3340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1027619, whole genome shotgun sequence.
ACCESSION   DASUYC010000118 DASUYC010000000
VERSION     DASUYC010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027619"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="VF276_01905"
     CDS             <1..641
                     /locus_tag="VF276_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605644.1"
                     /translation="LTLPTSDTAVRTSAFVLLSRAKVAAPLYHSLVVHEFFHVLQDAH
                     NALVWSRNSGTAAAPVWDAFWFMEASATWAEAYYDRTITWPQRAAGTYTYPWFRDFQQ
                     NLAALHASDPPDHMYGAFIWPFFMEQETGGPTLMGDAWRAFEPLGAGDFDGAMTALDR
                     TFAFDTHFADFAVRNLNSEFDGQDPIRTHYRDRDSLFPEGVMPRPNEQDAFV"
     gene            684..1622
                     /locus_tag="VF276_01910"
     CDS             684..1622
                     /locus_tag="VF276_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605645.1"
                     /translation="MAPLQAAYYHYAVLSGIKQIRLNFAQLAPAAHLKARAVVRVRGE
                     NGAPSQWSLRDLSTTSENVLCDVDEIWIVLSNTSFSYDVSQTIRSSFRVRALAAPCTC
                     DEALLVRSGWAGTYGLSWNDTASRTVTGGMEQAHVQHLANLQLRLSDWNPDPMTQDPR
                     WEAWVLSNNISGDAHANDTDTWTMNGNATTYSNIGHGPPDFLTFARLTLRAATCTYDL
                     QIMVSTATTRMPGGNEENALLGLLNLTGLPLPVDGAEQRLQDYRNVHATLTGSDFQVG
                     ATAGVTGLERIKGPDGMNDATVTWDFTPIAPAAAER"
     gene            complement(1668..2282)
                     /locus_tag="VF276_01915"
     CDS             complement(1668..2282)
                     /locus_tag="VF276_01915"
                     /inference="COORDINATES: protein motif:HMM:NF025636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX6605646.1"
                     /translation="MVAQDRRVRRTQQLLARALVALTLEKGYEAVTIRDLTDRADIGY
                     ATFFRHYRDKDELLQDVLDVVLEELTGLLSGAVPDGDPAAVGTLVFRYVQEHTEVVRV
                     LLGSRQVLQRLVAVATDLTMREQTARPDSPVPLEIAAHHIVSATIALVEWWLDHGMPY
                     PPEQMGRLYRDLIAGPTGALVFAPPALAPSGDGAQPGPASAQHT"
     gene            2487..>3340
                     /locus_tag="VF276_01920"
     CDS             2487..>3340
                     /locus_tag="VF276_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX6605647.1"
                     /translation="MSLVQDNPSTQAPARCPVDHHAPAQKKSVRPRSEGTARVEQDSR
                     GVWHVRGFEEVRTVLRSSETQQAGFKAELVTAGNALSNRPILYQEGKVHQEQRTKTAR
                     FFTPKTVSTQYRRMMERLADRLVARARRQPQIDLTRMGLTMAVAVTAQVVGLTNSRRR
                     GMDRRLDAFFEQTTVSRHRGALGHLMQMVNLRRMLWFYWLDVRPAIAARRITPQEDVI
                     SHLLAQNYNDREILTECVTYGAAGMVTTREFISVAAWHLLDHPDLRARYLAAPEAERV
                     AILHEMLRL"
BASE COUNT          587 a         1128 c         1092 g          533 t
ORIGIN      
        1 gcctgacgct gccgacctcc gacacggccg tccgcaccag cgcctttgtg ctgctgtcgc
       61 gggcgaaagt ggccgcgccg ctctatcact cgctggtggt ccacgagttc ttccacgtcc
      121 tgcaggacgc gcacaacgcg ctggtgtggt cgcgtaacag cggcaccgcc gccgcgccgg
      181 tgtgggacgc gttttggttc atggaagcct cggccacctg ggccgaggcc tactacgacc
      241 gcaccatcac ctggccgcag cgggccgccg gcacctatac ctacccctgg ttccgcgact
      301 tccagcagaa cctggccgcg ctacacgctt cggacccgcc cgaccatatg tacggcgcct
      361 tcatctggcc cttcttcatg gaacaggaga ccggcggccc gaccttgatg ggggatgcct
      421 ggcgcgcgtt tgaaccgctc ggcgcgggcg acttcgacgg agcgatgacc gcgctggacc
      481 gcaccttcgc cttcgacacg cactttgccg acttcgccgt gcgcaatctc aacagcgaat
      541 tcgacggcca ggaccctatc cgcacccact accgggaccg cgactccctc ttcccggagg
      601 gcgtgatgcc gcgcccgaac gagcaggatg cgttcgtcta gcccatggat cccgcaaatc
      661 ccggcaagga ggtcgcggcg actatggcgc cgttgcaggc cgcttactac cactacgcgg
      721 tgctgtcggg tatcaagcag atccggctca acttcgcgca actggccccg gcggcccacc
      781 tcaaggcccg cgcggtggtg cgggtgcgag gcgagaacgg cgcgccgagc cagtggagtc
      841 tgcgcgacct ctcgaccacc agcgaaaacg tcctctgcga cgtggacgag atctggatcg
      901 tgttgagcaa cacgtcgttc tcctacgacg tgagccagac gatccggtcg agctttcgcg
      961 tgcgcgcgct ggcggcgccg tgcacctgcg acgaggcctt gctggtccgc agcgggtggg
     1021 cgggcaccta cggcctgtcc tggaacgata ctgcgtcgcg caccgtcacg ggcggcatgg
     1081 agcaggcgca cgtgcagcac ttagccaacc tgcagctccg gctctccgac tggaacccgg
     1141 acccgatgac ccaggacccg cgctgggaag cctgggtgtt gagcaacaat atctcgggcg
     1201 acgcccacgc caacgacacc gatacctgga cgatgaacgg caacgccacc acctactcga
     1261 atatcgggca cgggccgccg gatttcctga cctttgcccg cctgacgctg cgcgcggcca
     1321 cctgcaccta cgacttgcag atcatggtca gcacggcgac gacgcgtatg cccggcggca
     1381 acgaggaaaa tgcactgctg ggcctgctca atctcaccgg gctgccactg cccgtggacg
     1441 gggccgaaca gcggctgcaa gactaccgga acgtgcatgc cacgctgacg ggctctgact
     1501 tccaggtcgg cgcaaccgcg ggcgtgaccg ggctcgaacg catcaagggg ccggacggca
     1561 tgaacgacgc gaccgtcacc tgggacttca cgcccattgc gcccgcggcg gctgagcgat
     1621 agtttacata agtataagag cggcgctgcc ggcaacggca gcgccgctca ggtgtgctgc
     1681 gcgcttgccg ggccgggctg ggcgccgtcg ccgctagggg cgagggccgg cggcgcgaac
     1741 accagcgcgc cagtggggcc ggcgatcaag tcgcggtaga ggcggcccat ctgctcgggc
     1801 gggtagggca taccatggtc cagccaccac tcgaccagcg cgatggtggc gctgacgatg
     1861 tgatgggcgg cgatctccag tggcaccggg ctgtcggggc gggcggtttg ctcgcgcatg
     1921 gtcagatcgg tcgccacggc gacgaggcgt tgcagcacct ggcggctgcc cagcagcacg
     1981 cgcaccacct cggtgtgttc ctgcacatag cgaaacacca gcgtgcccac cgccgccgga
     2041 tcgccgtcgg gcacggcccc ggacagcagg ccggtcagtt cctccagcac cacatcgagg
     2101 acatcctgca ataactcgtc cttgtcgcgg tagtggcgga aaaaggtggc gtagccgata
     2161 tcggcccggt ccgtgaggtc gcgtatggtc acggcctcat agcccttttc cagcgtcaat
     2221 gcgaccagcg cgcgcgccag caactgctgc gtgcgccgga cccggcggtc ctgtgctacc
     2281 atgcctgcct ccccgctgcc tgatgttcat tttgcctatc gttgtgcata tctatcatac
     2341 catcataacc gtccgttgtg taacgtcttg actgttgcat cgttatgagc tatagtataa
     2401 cataagatac tcagttaacc aaaacggtaa ggtactatca taaccgggtg ccctactgga
     2461 accagcgaaa ggaatgcgga ttcgctatgt cgcttgtgca agataaccca tccacgcagg
     2521 ccccggcgcg ctgccccgtc gaccatcacg ccccggcgca gaagaaatcg gtgcgcccgc
     2581 ggtccgaagg cacggcgcgc gtggaacagg acagccgcgg ggtgtggcat gtgcgcgggt
     2641 ttgaggaggt gcggacggtc ctgcgcagca gcgagacgca gcaggccggc ttcaaggccg
     2701 aactggtgac ggcgggcaac gcgctgagta atcgccccat cctctaccag gagggcaaag
     2761 tccaccagga gcagcgcacc aagacggcaa ggtttttcac gcccaagacg gtgagcacgc
     2821 agtaccggcg aatgatggaa cggctggccg accggctggt ggcccgcgcc cggcggcaac
     2881 cgcagatcga tctgacccgg atgggtctga ccatggcggt ggcggtgacg gcgcaagtcg
     2941 tggggctgac caacagccgg cggcgcggca tggaccggcg cctggacgcc ttcttcgagc
     3001 aaaccaccgt ctcgcggcat cgcggcgcgt tgggccacct gatgcagatg gtcaatctgc
     3061 ggcggatgct gtggttctac tggctggacg tgcgcccggc catcgcggcg cgacggatca
     3121 cgccgcagga ggacgtgatc tcgcacctgc tggcgcagaa ctacaacgac cgcgagatcc
     3181 tgaccgagtg cgtgacctac ggcgcggcgg gcatggtcac gacccgcgag tttatcagcg
     3241 tggccgcctg gcacctgctg gaccaccccg acctgcgggc gcgctacctg gccgcgccgg
     3301 aagccgagcg cgtggcgatc ctgcacgaga tgttgcggct
//
LOCUS       DASUYC010000119         2832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_251126,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000119 DASUYC010000000
VERSION     DASUYC010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251126"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..1151
                     /locus_tag="VF276_01925"
     CDS             231..1151
                     /locus_tag="VF276_01925"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6605648.1"
                     /translation="MRIRMRPYAGEADQQRMSDLVHAAPAGNIHLADLPYRLSSWAFD
                     DPGNIGLWEDGTGHLLAWAVLQTPFWAIDYAYRPDVGDYGIHPMILTWAVAHAAAVVG
                     TANGRPAWYVSVFADQADRQGDLERAGFVRQADWSQVLMERPAAELVPPAILPDGFTV
                     RPLAGPAEAAAYTELHRAAFRSENMTAAWRARTLARREYIADLDLVVVAPDGRLAAFC
                     VGWLDRHGPGSTVLGQVEPLGVHPDFQGQGLGRAVLLEAMRRMQAHGATRLVVETDTY
                     RDTAFGLYQSVGFGVAHDIWVYGRAFTAAA"
     gene            complement(1148..1507)
                     /locus_tag="VF276_01930"
     CDS             complement(1148..1507)
                     /locus_tag="VF276_01930"
                     /inference="COORDINATES: protein motif:HMM:NF019459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1634 domain-containing protein"
                     /protein_id="HEX6605649.1"
                     /translation="MHETVDKTLYQGVQRTLVGGMILSFGLMALGLLGLALDPAAGAR
                     ATQVVPLDRLPAALLALDPAALLDLGVLALMFIPVVHLAVALLTFVRRREPRYAVAAL
                     VVLALLAGSAILAFTRH"
     gene            complement(1488..2003)
                     /locus_tag="VF276_01935"
     CDS             complement(1488..2003)
                     /locus_tag="VF276_01935"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX6605650.1"
                     /translation="MPEATHLADALLGLFAHDHGWFTPFTRAVAGLTAAQAAWEPPEG
                     SARSIWAIVNHLGFWHDVVLHDLHGLPLAGSGLDTAQDWPPPGPPTDEPGWEQACRRV
                     IALNSALAGSIAALADEELDQPVRPGGAELWQIVQGLIGRSSFYTGEICSIRRRQGLW
                     PPREHDARNGG"
     gene            complement(2112..>2832)
                     /locus_tag="VF276_01940"
     CDS             complement(2112..>2832)
                     /locus_tag="VF276_01940"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX6605651.1"
                     /translation="SSTYVRTRLTNIRLAVLLGTATVAAAIGASLVAQAIDGRILRGL
                     FAFLLLYTAVTMIRRARPAAQPAAQAAPAPAPKGAAKEAPAPPASDGWNLGGSYRDAR
                     LGQVAYQATRVPLGMGASLLGGTISGLLGVGGGIVQVPVMHLLMGVPLKVATGTSSFL
                     IGITAAASALIYYASGRINPVLAAPAALAVFVGARLGAYLVQRLQSTTLKRIFGVVAL
                     LVAVQMILQAMDACPWCPPGR"
BASE COUNT          448 a          950 c         1015 g          419 t
ORIGIN      
        1 ctgggttagc aggcaggctc aagttatcca ccgcaacggt ggataagttc gtggataagc
       61 ttgtggataa aggggcttga ctcaccacgg gatcggtgct atactggcat cacttcagta
      121 aattggtctt agcaataaga gcaagataca ggagcacaaa ggccggagaa tccgcaagga
      181 ttgtccggct tttggtttgt ccgcgcatcg ctcggaggga gtggttcagg atgcggatcc
      241 gcatgcgccc gtatgccggc gaggccgacc agcagcgtat gagcgacctt gtccacgccg
      301 ccccggcggg caacatccac ctcgccgatt tgccctaccg gctcagctcc tgggcgttcg
      361 atgatcccgg caatatcggc ctctgggaag acggcaccgg gcatctgctg gcctgggccg
      421 tattgcaaac gccgttctgg gccatcgact acgcgtatcg cccggatgtg ggcgattacg
      481 gcatccaccc catgatcctc acctgggcag tggcgcatgc cgcggcggtg gtcgggaccg
      541 cgaacgggcg cccggcctgg tacgtcagtg tgtttgccga tcaagcggat cgccagggcg
      601 acctggagcg ggcggggttc gtccgccagg ccgactggtc gcaggtactc atggagcgcc
      661 ccgccgccga actcgtgccg cctgctatcc tgccggacgg cttcaccgtg cgcccgctgg
      721 ccgggcccgc cgaggcggca gcctacacgg aactgcatcg cgccgccttc cgcagcgaga
      781 atatgaccgc cgcgtggcgc gcccgcaccc tggcgcggcg cgagtacatc gccgatctcg
      841 atctggtggt cgtcgcgccg gatgggcggc tcgcggcatt ttgcgtgggc tggctggacc
      901 gccacgggcc gggcagcacg gtgctgggcc aggtggaacc gctgggcgtg catcccgatt
      961 tccagggcca ggggctaggg cgggcggtgc tgctggaagc gatgcggcgc atgcaagcgc
     1021 acggcgccac ccggctcgtg gtggagacgg acacctaccg ggacaccgcc tttggcctgt
     1081 accagtcggt gggcttcggg gtggcgcacg acatctgggt ctacgggcgg gctttcacgg
     1141 ctgcggccta atggcgcgtg aaggcgagga tggcgctccc ggccagcaac gccagcacga
     1201 ccagcgcggc cacggcatag cgcggctcgc ggcggcggac aaaggtcagc aacgcgacgg
     1261 ccaggtggac taccgggatg aacatgagcg ccagcacgcc caggtcgagc agggcggcgg
     1321 ggtcgagcgc cagcagggcc gcgggtaagc ggtcgagcgg gacgacctgg gtggcgcggg
     1381 cccccgccgc cgggtccagg gccaggccga gcagcccgag cgccatcagc ccgaagctga
     1441 ggatcatgcc gccgaccagg gtgcgctgca cgccctgata gagcgtctta tccaccgttt
     1501 cgtgcatcgt gctcccttgg cggccagagc ccctgccggc gccggatact acagatttcc
     1561 ccggtgtaaa agctgctgcg gccgatcagc ccctggacga tctgccagag ttcggcgccg
     1621 ccgggccgta ccggctggtc cagttcctcg tcggcgaggg cggcgatgct gccggccagc
     1681 gcgctgttga gggcaattac ccgccggcac gcctgctccc agcccggctc gtcggtgggc
     1741 gggccgggcg gcggccagtc ttgcgcagtg tcgaggccgc tgcccgctag cggtagccca
     1801 tgcaggtcgt gcagcaccac atcgtgccag aagccgagat ggttgacgat ggcccagatg
     1861 ctgcgggcgg acccctccgg cggttcccag gcggcctggg ccgcggtcag gccggccacg
     1921 gcgcgggtaa agggcgtgaa ccagccgtgg tcgtgcgcga acaggcccag cagagcatcg
     1981 gccaggtgcg tggcttccgg catcgtccgt cctccgctgg gtggcaggcc ggtttgcaac
     2041 cggtccctac atcgccgcca agcacgggcc ggtttcaaac cggcccctac atcgcaaaat
     2101 gacagtttcc gctagcggcc cggcgggcac caggggcagg cgtccatggc ctgcaagatc
     2161 atctgcacgg cgaccagcaa cgcgaccacg ccgaagatgc gcttcaaggt ggtgctttgc
     2221 aggcgctgca ccaggtaggc gcccaggcgc gcgcccacga acacggccag cgcggcgggc
     2281 gccgccagta ccgggttgat ccggccgctg gcgtaataga tcagcgcact ggccgccgcc
     2341 gtgatgccga tcagaaacga actggtgccc gtcgccacct tcagcgggac gcccatcagc
     2401 aggtgcatca ccggcacctg aacgatcccg ccgccgacgc cgagcaagcc cgaaatcgtg
     2461 ccgccgagca ggctggcgcc catgccgagc gggacgcgcg tcgcttggta ggccacctgc
     2521 cccagccggg cgtcccggta gctgccgccc aggttccagc cgtcggacgc gggcggggcc
     2581 ggtgcctctt tcgctgcccc tttaggggca ggcgcgggag cggcttgcgc cgccggttgg
     2641 gctgccgggc gggcgcggcg gatcatcgtc acggcggtgt agagcagcag gaacgcgaac
     2701 agcccgcgca ggatgcgccc atcgatagcc tgggcgacca gcgaggcgcc gatggcggcg
     2761 gctaccgtcg ccgtgcccag cagcacggcc agccggatgt tggtcagccg ggtgcggaca
     2821 taggtgctcg ac
//
LOCUS       DASUYC010000120         2441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_736013,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000120 DASUYC010000000
VERSION     DASUYC010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736013"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="VF276_01945"
     CDS             complement(<1..790)
                     /locus_tag="VF276_01945"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX6605652.1"
                     /translation="MSRFPLRMDLKTAQHDRDWDNILRYCRDTEAAPAFTGFWLFDHF
                     VPINGHVEGPCMDGWTLLAALAAATQRLRLGLMVGCNGYRYPAVLAKIATTVDQISGG
                     RLEMGLGAGWFELEYNMYGIPFPPPAQRIRELDEACQVIKLLWTEEVANFAGKYYTLT
                     AARHEPKPVQHPYPPFTIGGAGEKLTLRVVAKHAAIYNLSGGTPDDVRHKNAVLDDHC
                     AAIGRDPGEIRRSIQVHVRRPDDAATIREQIEPYLEVDIDHICIG"
     gene            complement(871..1713)
                     /locus_tag="VF276_01950"
     CDS             complement(871..1713)
                     /locus_tag="VF276_01950"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017238.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6605653.1"
                     /translation="MDNLYSTDYFRRRRMGQALTTPFDFQSPALKAYRRAARMLMVQP
                     GMRILDAGCGLGAGSWLLAMLGAQVTGVDVSADAIAWARETYGREPLRKGGTLDYLTA
                     DLTTWTPPDRYDAIIVADVLEHFPRAAGVDMLRRLLSALVATEQPSARGWADGLFLHL
                     PITANLIDWVLLVKNHLLLPRLRGEIIDHHGDTTHALRYGLLDVPQVAHAAGGQICRV
                     ELRIYKPRLRTVERLLLGGNHRTLTRFGGTVITDLDAILLPELVAALRAAPPDPEPPR
                     SPES"
     gene            complement(1767..>2441)
                     /locus_tag="VF276_01955"
     CDS             complement(1767..>2441)
                     /locus_tag="VF276_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605654.1"
                     /translation="GALVLVLFLGILAFVTINITLDIASGQGGEFTGPFFSAGLCGFA
                     SLVLCIGILYFLNAIRLAVLDLASAPVTYEGRIVQRMGGRGRGGAFWVVVGPPRTEES
                     ETPASLAAAPAFVPPVMSTEAAGPAPRDRGFVSSSGGSFGAQLQGAKVRSAPAESAGA
                     WRMPETRHKLAPGEVNLRVDKHIFEALVNGDRVQVVYSPHLQHVYYVRKRDPGGALVL
                     RNLSLL"
BASE COUNT          415 a          809 c          853 g          364 t
ORIGIN      
        1 agccgatgca gatgtggtcg atgtccactt cgaggtaggg ttcgatctgc tcccggattg
       61 tggccgcatc gtcgggcctg cgcacgtgga cctggatcga gcggcggatc tcgccggggt
      121 cgcggccaat ggcggcgcag tgatcgtcga gtacggcgtt cttgtggcgc acgtcatcgg
      181 gcgtgccgcc cgacaggttg tagatagccg cgtgtttcgc caccacgcgc agggtcagct
      241 tctcgccggc gccgccgatg gtgaagggcg ggtaggggtg ctgcaccggc ttcggctcgt
      301 gccgggccgc ggtcagggtg tagtacttgc cggcgaaatt ggcgacttct tccgtccaga
      361 gcaacttgat cacctggcag gcttcgtcca gttcgcggat gcgctgggcg ggtggcggaa
      421 acgggatgcc gtacatgttg tactccaact cgaaccagcc cgcccccagg cccatctcca
      481 ggcgcccgcc gctgatctgg tccaccgtgg tggcgatctt ggccagcacc gccgggtagc
      541 ggtagccgtt gcagccgacc atcaggccca ggcgcaggcg ctgagtcgcc gcggccagcg
      601 ccgcaagcag cgtccagccg tccatgcacg ggccttcgac atggccgttg atcggcacga
      661 agtggtcgaa cagccagaag ccggtaaagg ccggcgccgc ctcggtgtcg cggcaatagc
      721 gcagaatatt atcccagtcg cggtcgtgct gggccgtctt cagatccatc cgtagcggaa
      781 atcggctcat ggctcacctc cagcagcaaa gcagcagccc ggatgccggc cggtcccggc
      841 cccgcgccag ggcgggtgac gcacagggtt ttagctctcc gggctgcgcg gcggttcggg
      901 gtctggcggg gccgcacgca gggccgccac cagttccggc agcaggatcg cgtccaggtc
      961 ggtgatcacg gtgccgccga accgcgtcag ggtgcgatgg ttgccgccca gcaacagacg
     1021 ttccacggtg cgcaagcgcg gcttgtagat gcgcaactcc acgcggcaga tctggccgcc
     1081 ggcggcgtgg gccacctgcg gtacgtcgag caggccgtag cgcagcgcgt gggtcgtgtc
     1141 gccgtggtgg tcgataatct cgccgcgcaa ccggggcagc aacaggtggt tcttcaccag
     1201 gagcacccag tcgatcaggt tcgcggtgat gggcaggtgg agaaacaagc catcggccca
     1261 gccgcgcgcc gaaggctgtt cggtagccac cagagccgaa agcaggcggc gcaacatatc
     1321 gacgccggcc gcacgcggaa agtgttccag cacatcggcg acgatgatgg cgtcgtagcg
     1381 gtcggggggc gtccaggtgg tgaggtcggc ggtaagataa tcgagggtgc cgcccttgcg
     1441 caacggttcc cgcccgtaag tctcgcgcgc ccaggcaatc gcgtcagccg acacatcgac
     1501 gccagtcacc tgcgcgccga gcatcgccag cagccaggac ccggcgccca acccgcagcc
     1561 ggcatcgagg atgcgcatgc cgggttgcac catcagcatc cgcgcggccc ggcggtaggc
     1621 tttcagggcc ggcgactgga agtcaaacgg ggtcgtcagc gcctgcccca tgcggcggcg
     1681 tcggaagtag tcggttgaat agaggttatc catagagagc taatcgccgg gagccgcccc
     1741 caccaccggc gcgggccagg actcccctac aggaggctca ggttgcgcag caccagcgcg
     1801 ccgccggggt cgcgcttgcg tacatagtat acatgctgca agtgcgggga gtacaccacc
     1861 tggacgcggt ccccgttgac gagcgcttcg aagatatgct tgtcgacgcg caggttcact
     1921 tcgccgggcg ccagcttgtg ccgggtctcc ggcatccgcc aggcgccggc cgactcagca
     1981 ggggcgctgc gtaccttggc gccctggagt tgcgcgccga aggatccgcc gctgctgctg
     2041 acaaaaccgc gatcccgcgg ggccgggccc gccgcctcgg tactcatgac cggcggcaca
     2101 aaggccggcg cggcggccag agaggccggc gtctcgctct cctcggtgcg cggcgggccg
     2161 acaacaaccc agaaggcccc gccccgaccc cgcccgccca tgcgctgcac aatgcggcct
     2221 tcgtaggtca ccggggcgga ggcgaggtcc agcacggcga gccgaatcgc gttcaggaag
     2281 tagaggatac cgatgcacaa aaccagcgag gcgaagccac acaggcccgc gctgaaaaac
     2341 ggcccggtga actcgccgcc ctgcccgctg gcgatgtcga gcgtgatgtt gatcgtgaca
     2401 aaggcgagga tgccgagaaa gaggaccagc accagcgccc c
//
LOCUS       DASUYC010000121         4684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_530966,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000121 DASUYC010000000
VERSION     DASUYC010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4684
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530966"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VF276_01960"
     CDS             complement(<1..408)
                     /locus_tag="VF276_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605655.1"
                     /translation="MTDEKRTPPERREALANRPAPTRRARQGKSRGTAHMKQIITAAS
                     IASVVGGWALMAGQDADSSAGAAAVAVAPATATATDQPAATASPTAQVAITLPAGEAT
                     VTTQAPATATATLQPEATATAAATSTPVPTATTP"
     gene            886..1302
                     /locus_tag="VF276_01965"
     CDS             886..1302
                     /locus_tag="VF276_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605656.1"
                     /translation="MTNRPAQQKPDPAIGRNIRRARRGPAGDKAKGLVVAVSLATTLM
                     GWSIFAHEDAQQAALQAADTAPVAAVAETTAASISTAQSSSALPTATATAVPIVTANA
                     GTASNQSSSTTTTVSTSQSSSTTTNPVAVARTQSSR"
     gene            1334..2170
                     /locus_tag="VF276_01970"
     CDS             1334..2170
                     /locus_tag="VF276_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605657.1"
                     /translation="MQLTPQSNAAPRQTGIWLWLVAGAAAVGAIALAGLPALANSFAG
                     TAPHAYWYISRACAFVAFGLLWLSMLAGLGITSRLARYWPGLPGSFELHRFTALLGLG
                     FAAMHALVLLGDPYMNYTLAQLLVPFMSTEYRQAWVGFGQLAIYLLAVVAFSFYVKDR
                     LGIHAWRLIHMLSFALFLMTIIHGVAGGTDSGNLWAQALYWIAALSVLGLSVYRVPAV
                     RRGRAKADPAAKVLVATAGRAPVPPAAPVTGASPRMVRAGVLVPSGTAYVGRSRGSER
                     LE"
     gene            2489..3262
                     /locus_tag="VF276_01975"
     CDS             2489..3262
                     /locus_tag="VF276_01975"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HEX6605658.1"
                     /translation="MNGHDARIEIVIPVYNEAAQLAASIETLRAYLLLELPYRFQITI
                     ADNASTDATPAIARALAARYPEVQVLRLEQKGRGRALRAAWGASRADVVSYMDVDLST
                     GLPAFLPLIAPLVSGQSDLAIGTRLARGAEVTRSPKREVISRVYNLLIKPAFFNRFSD
                     AQCGFKAARADVIKRLLPLIENNAWFFDTELLLLAEHNGLRIAEVPVRWIEDPGTSVH
                     LRSTIAEDLRGLWRVRRAFWRGQGRLPAPRRLDAVPVSR"
     gene            3437..4540
                     /locus_tag="VF276_01980"
     CDS             3437..4540
                     /locus_tag="VF276_01980"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX6605659.1"
                     /translation="MSVFSTSARRAPHPGPVTGAGIGGPRPRPALAVAAHDGAEIGLG
                     RRLRDWKTLAGFALSAAIILVFVLTAHLDLGRIWATVRTADGRYLAAALAVYFGAFLL
                     RGLRWRLLLRRADLGAAIRLPALGGLVEMIFLSWFVNCLVPAKLGDAYRAYLLKQDTG
                     AGMDRTLGTVVGERMADVLALVVLLLGSGLLILGDLAGAGGNLPLILLLGAGLAGAIA
                     GGAMALRMGAGRVAARLPERRRVVFERFVAALLGTFRRDIQAPLYGLTALIWAGEVIR
                     IWLVLQSFHITGLAPGVIIFTALAGSLLSTIPFTPAGLGAVEGASVAILAAFGVAGSL
                     AGAVAVMDRVINYWSNIPVGALLYLARRRRWRA"
BASE COUNT          682 a         1527 c         1648 g          827 t
ORIGIN      
        1 gggggtcgtg gcggtgggca cgggcgtgct ggtggcggcc gccgtggccg tggcctccgg
       61 ttgcaaggtc gcggtggccg tggccggcgc ctgcgttgtc accgtcgcct cgcccgcggg
      121 cagggtaatc gcgacctgcg ccgtaggcga cgccgtggcc gccggctgat cggtcgcggt
      181 ggccgtggcc ggcgctaccg ccacggcggc cgcaccggcg ctgctgtcgg catcctgccc
      241 ggccatcagc gcccagccgc cgaccacgga tgcgatcgat gcggcggtga tgatctgctt
      301 catgtgcgcc gtcccgcggg acttgccctg gcgggcgcgt cgggtcgggg caggccggtt
      361 ggcgagtgcc tcgcggcgct cggggggtgt gcgcttctca tcagtcatgt ccatgttcct
      421 cgcttggtct ctttctagtg ctgccgataa cccttgtcac tctgagggga gcgaagagtc
      481 tcgtctcttg tcctaacagg acggagattc ttcgctacgc tcagaatgac ggtttccttt
      541 tgtagtgttg ccaatagccc ttgtcactct gagcgcagcg aagagtctcg tctcttgtcc
      601 taacaggacg gagattcttc gctgtgatca gcatgacggc tcctggtggg ccgtcactac
      661 gtgtatttcg cgccgcgccg gtgcgcggta cctgattagt gtagggcagg tgcgttagag
      721 gatggccggg gaagcattag aggttgctga gattcgcccc ggcgaatcga gggcgcggtt
      781 tctcagtgtt tcctaatttt tccgggccgg gttttaagcc ggccccggta gactcggacc
      841 atgcaatcat cgttcggggt atcccgaaat caaggagaca ctgacatgac aaacagaccc
      901 gcacaacaga aacccgatcc ggccattggc cgcaatatcc ggcgcgctcg ccgcggcccc
      961 gccggcgaca aagccaaggg cctcgtcgtc gcggtctcgc tcgccacgac gcttatgggc
     1021 tggagcatct tcgcccacga ggacgcgcag caggcggcgt tacaggcggc ggatacggcg
     1081 ccggtggccg ccgtcgcgga gaccacggct gcatccatca gcaccgcgca gagtagcagc
     1141 gcgctgccaa cagccaccgc cacggcggtg cccatcgtaa cggcgaacgc cggcaccgcc
     1201 agcaaccaga gcagcagcac gaccacgacg gtcagcacta gccagagcag cagtacgacc
     1261 acgaacccgg tagctgtcgc ccggacgcag tcgtcccgct aacgggcgac ggatcagaac
     1321 aggagaaatg acgatgcaac ttacccctca atccaacgcg gcaccccggc aaacaggcat
     1381 ctggctctgg ctggtggccg gggcggcggc ggtgggcgcg atcgcgctgg ccgggctgcc
     1441 cgctctcgcc aactcgtttg ccggcacggc gccgcacgcc tactggtata tctcgcgggc
     1501 ctgcgccttc gtcgcctttg ggctgctgtg gttgtcgatg ctggcgggcc tgggcatcac
     1561 cagccggctg gcgcgctact ggccgggcct gccgggcagc ttcgagttac accgcttcac
     1621 ggccctgctg ggcctgggct tcgccgccat gcacgcgctc gtgctgctgg gcgacccgta
     1681 catgaattac actctggcgc aactgctggt gccgttcatg agtaccgagt accggcaggc
     1741 atgggtaggc ttcggccaac tggcgatcta cctgctcgcg gtggtggcgt ttagcttcta
     1801 tgtcaaggat cgcctgggca tccacgcctg gcggctgatc cacatgctga gcttcgcgct
     1861 gttcctgatg acgatcatcc acggcgtggc cggcggcacg gacagcggca acctgtgggc
     1921 gcaggcgctg tactggatcg cggcgctgag cgtgctcggt ctctcggtct accgggtgcc
     1981 ggccgtgcgc cggggccggg cgaaagccga cccggcggcg aaggtcctgg tcgccacggc
     2041 gggccgggcg ccggtgccgc ccgccgcccc cgtgacgggc gcatccccgc gcatggtgcg
     2101 ggcgggcgtg ctggtgccga gcgggacggc ctacgtgggg cggtcgcgcg ggtcggagag
     2161 actggaataa acggacgtgc gccggacgaa gtatctgccc ggaggggatc ggccggcctg
     2221 gccggggtct cctccgggct tcgtgttggc gacgcgatac ggtgggtaaa tctcagcgaa
     2281 ttctaattct tctccgcctt cttctaaagc cggaggtcta cactggaatt agcatagatg
     2341 ttacgagccg gacagacgac ctgcccggcc catgaagcaa atcccgcgaa gtggggcgcg
     2401 cgttccggcc cgaaagcccc cttgcactta atcggcaagg atctcccgcc gcgcgggggc
     2461 gccatgaaca gcgaaaggac caatgaacat gaacggccat gatgcccgca tcgagatcgt
     2521 gatcccggtg tataacgagg cggcgcagtt ggcggcgagc atcgagacgt tgcgcgcgta
     2581 cctgttgctc gaattgccct accggttcca gatcaccatc gccgacaacg ccagcaccga
     2641 tgccaccccg gcgattgcgc gggcgctggc ggcgcgctat cccgaagtgc aggtgctgcg
     2701 cctggagcag aaaggccgcg ggcgcgcgct gcgggccgcc tggggcgcca gccgggccga
     2761 tgtcgtcagc tacatggatg tcgacctctc gacggggttg cccgcctttc tcccgctgat
     2821 cgcgccgctg gtcagcggcc agagcgacct agccatcggt acgcggcttg cgcggggggc
     2881 cgaagtcacc cgctcgccca aacgcgaagt gatctcccgc gtgtataacc tgctgatcaa
     2941 gccggctttc ttcaaccgct tcagcgatgc ccagtgcggc ttcaaggccg cccgcgccga
     3001 cgtgatcaag cgcctgctgc ccctgatcga gaacaacgcc tggttcttcg atacggaact
     3061 gctgttgctg gccgagcaca acggcttgcg catcgccgag gtaccggtgc gctggatcga
     3121 ggaccccggc acgtcggtgc atctgcgcag caccatcgcc gaggatctgc gcgggttgtg
     3181 gcgggtgcgg cgtgctttct ggcggggtca ggggcgcctg ccggcgcccc ggcggctcga
     3241 tgccgtgccc gtgagccggt agcctgtcgt ttccaccgtg gtcgtcatga acaattgcgg
     3301 agtttgtttg aggggcaccg tcattctgag cgcagcgaag aatctccgtc tagtagacca
     3361 aacgacgaga ctcttcgctg cgctcagagt gacaaacgcc tggggataaa cgattgagta
     3421 ccaaacgagg ctggacatga gtgtattttc gacaagcgcg cgccgggcgc cgcatcctgg
     3481 cccggttacg ggagccggga tcggcggccc gcgcccgcgc cccgccctgg ccgtggcggc
     3541 ccatgacggc gcggagatcg gcctgggccg ccgcctgcgc gactggaaga cgctggcggg
     3601 ctttgccctc agcgcggcca tcatcctggt ctttgtgctg acagcgcacc tggatctggg
     3661 ccggatctgg gccaccgtgc gcaccgccga cgggcgctat ctggcggcgg cgctggccgt
     3721 ctatttcggc gcctttctgc tgcgcgggtt gcgctggcgg ctgctgttgc gccgggccga
     3781 tctcggcgcc gccatccggc tgccggcgct gggcgggctg gtggaaatga tcttcctctc
     3841 gtggttcgtc aactgcctgg tgcccgccaa gctgggcgac gcctaccgcg cctacctgct
     3901 caagcaggac accggcgccg gcatggaccg gacgctgggc accgtggtgg gcgagcgcat
     3961 ggcggacgtg ctggccctgg tggtcctgct gctgggcagc gggctgctga tcctcggcga
     4021 cctggccggc gccgggggca acctgccgct gattctcctg ctcggcgcag ggctggcggg
     4081 ggccatcgcg ggcggcgcga tggcgctgcg catgggggcg gggcgggtag ccgcccggct
     4141 gccggagcgc cggcgcgtgg tgttcgagcg cttcgtcgcc gccctgctgg gcacgttccg
     4201 gcgcgacatc caggcgccgc tgtatggcct gaccgcgctg atctgggccg gcgaagtcat
     4261 ccgcatctgg ttggtgctgc aaagcttcca cattaccggg ctggcgccag gggtgatcat
     4321 cttcaccgcg ctggccgggt cgctgctgag cacgatcccg ttcacgcctg ccgggctggg
     4381 cgcggtcgag ggcgcgtcgg tcgccatcct ggccgccttt ggcgtcgccg gcagcctggc
     4441 cggcgcggtc gccgtgatgg acagggtgat caactattgg agcaatattc cggtgggcgc
     4501 gctgctgtac ctggcccgcc ggcggcggtg gcgggcatga gcgcccctgt cctggcccga
     4561 ccttgccgcg gctaatcagc cggtagccga ggatggtata ctatatggta aggtaaacgg
     4621 gttgaaccgc gcggcggcac cggcggcgcg cggcgggggc aaggattatc atgcaaaatc
     4681 gggt
//
LOCUS       DASUYC010000122         2731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_89766,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000122 DASUYC010000000
VERSION     DASUYC010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89766"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /locus_tag="VF276_01985"
     CDS             complement(<1..1015)
                     /locus_tag="VF276_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605660.1"
                     /translation="MTASPSLPAAPPAPPAVGENPPTAAEVYRERCARFGGLRDFYTD
                     RWNLIANVRLGTFVLAAIGLGWGIWQDIPPLTWLGGLLLVAFAVLVNYHNRLDRQRRR
                     YAELWAINDEASKRLARDWESLTPRFTAQAPPDHPYAGDLDIFGRASLFQLLESMGTA
                     IGARTLERWLLAPAPPAEVRERQPAVAELAPLIDLRDALALSGRMMGNPPPDPEPFLA
                     WAESAPWLARHQSLLWAGRVSTVLLWVTFAAWAAGLTRYPFWAIFLVANIVLVRVLGV
                     RATMTLERLAAGEDSFRHYAESFALLGEADFRAPRLRHLQSVLSAGGLTAHQQMRRLH
                     TFAR"
     gene            complement(1118..1834)
                     /locus_tag="VF276_01990"
     CDS             complement(1118..1834)
                     /locus_tag="VF276_01990"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX6605661.1"
                     /translation="MTHLYLIRHGEAVGAVQRRVADAIPDAGLTPLGRTQAARLRDRL
                     AATHEIAADVLIASSFPRARETAEIIAPALGHLPLLIDDEVQELRTGEADGLPWDEFN
                     ARYGPHDFDQDPFTPVAPGGESWSHFKLRVGAALHRIIREHAGKTIVVVCHGGVIDGS
                     FLYFFGLPTLVHSSAGFLTHNTSITEWEEYDRRGTRRWRLLRYNDDLHLRDNIRWSGV
                     SAEAETGADAPAVPLPTEAR"
     gene            complement(1923..>2731)
                     /locus_tag="VF276_01995"
     CDS             complement(1923..>2731)
                     /locus_tag="VF276_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605662.1"
                     /translation="PRFLAAALIVLLVFSVAFLFPATPLPLLFLYNLISAVVILGIAA
                     AFLLRRTGTVALRVTVALFGLAYLGYYLYALAAILAQGFGLPVGEFGVAANAAGEAAA
                     MTAGISLLWTAGLFRRAPGVPRLHPLWLVLAGLAGAVVLIAPLFETWLQGVATQFSVG
                     FTLFLPVPVSALALAAYCYALLAANSRRGPGITGLPWAWDMGAALCLLPAAGYQLQLN
                     YQHLLLAAVLLLITGTIRPLSSAVPRAESAPATAPAAWPEGRAAEQGPVR"
BASE COUNT          438 a          906 c         1042 g          345 t
ORIGIN      
        1 ggcgggcgaa ggtgtgcagc cggcgcatct gctgatgggc cgtcaggccg cccgcgctga
       61 gcacgctttg caggtgccgg aggcgcggcg cccggaaatc ggcttcgccc agcaaggcga
      121 aggattcggc gtagtggcgg aaggagtcct ccccggcggc gagccgttcc agggtcatgg
      181 tggcgcgcac gcccagcacg cgcaccagca cgatgttggc gacgaggaag atggcccaga
      241 acgggtaccg ggtcagcccc gccgcccagg cggcgaacgt cacccagagc aggaccgtgc
      301 tgacacgccc ggcccagagc aggctctggt gccgcgccag ccacggcgcc gactcggccc
      361 aggccaggaa cggttcggga tcgggtggcg ggttgcccat catccgcccg ctgagggcca
      421 gcgcgtcgcg cagatcgatc agcggcgcca gttcggccac cgcgggttgc cgctcgcgca
      481 cctcggcggg cggcgcgggg gccagcagcc agcgttcgag cgtacgcgcg ccgatggccg
      541 tgcccatcga ctccagtagt tggaagagcg aggctcgccc gaagatgtcc aggtccccgg
      601 cataggggtg atcgggcggc gcctgggccg tgaagcgggg cgtcaggctt tcccagtcgc
      661 gggccaggcg cttgctcgcc tcgtcgttga tggcccagag ttcggcgtag cggcggcgct
      721 gccggtccag ccggttgtgg tagttgacca gcacggcgaa ggcgaccagc agcaatccgc
      781 ccagccaggt caggggcggg atgtcctgcc agatgcccca gcccaggccg atcgccgcca
      841 gcacgaacgt gcccaggcgc acgttggcga tcaggttcca gcggtcggta tagaagtcgc
      901 gcaggccgcc gaagcgcgcg caccgttcgc ggtagacctc cgcggcggtg ggcggattct
      961 ccccgactgc cgggggcgcg gggggcgcag cgggcaagga tgggctagcg gtcatcgaca
     1021 gagccgccgc catagcggga cataatagca gtatttcatg tcgtagaata ccatgatcgg
     1081 cctattgctg caatgcccgc cgcctgatcg ggcgccccta gcgcgcttcc gtcggcaggg
     1141 ggacggcggg ggcgtccgcg ccagtctcgg cttcggcgga gacgccgctc cagcggatat
     1201 tgtcgcgcag gtgcaggtcg tcgttgtagc gcagcagccg ccagcggcgg gtgccccgcc
     1261 ggtcgtattc ctcccactcg gtgatcgacg tgttgtgggt gaggaagccg gcagacgaat
     1321 gaaccagcgt gggcaggccg aagaagtaca ggaacgagcc gtcgatcacc ccgccatggc
     1381 agacgaccac gatggtcttg ccggcgtgct cgcggatgat gcggtggagc gcggcgccca
     1441 cgcgcagctt gaaatgcgac cagctttcgc cgccgggggc caccggcgtg aagggatcct
     1501 ggtcgaagtc gtgcgggccg tagcgggcgt tgaactcgtc ccagggcagc ccgtcggctt
     1561 cgccggtgcg caattcctgc acctcgtcgt cgatcagcag gggcaggtgg cccagggcgg
     1621 gggcgatgat ttcggcggtc tcgcgggcgc ggggaaagct gctggcgatg agcacgtcgg
     1681 cggcgatctc gtgggtagcc gccagccggt cgcgcaggcg cgcggcctgg gtccgcccca
     1741 gcggcgtcag gcccgcgtcg gggatggcgt cggcgacccg gcgctgaacg gcgcccacgg
     1801 cttccccgtg gcggatgaga tagagatgcg tcacgaatcc aggtcctcct gtatagcgaa
     1861 ggggcgcaac cgcgtgcgcc cctcctgctg cgtgctgccg tgcggccctg ccgggggcgc
     1921 gcttagcgga ccggcccctg ttcggcggcg cggccttcgg gccaggcggc gggcgcagtg
     1981 gccggagcgc tctcggcgcg cgggacggcg ctgctcagcg ggcggatggt gccggtgatg
     2041 aggagcagaa cggcggccag cagcaggtgc tggtaattca gttgcaactg gtagcccgcc
     2101 gcgggcagca ggcagagcgc cgcgcccata tcccaggccc acggcaggcc cgtgatgccg
     2161 ggcccccggc ggctgttggc ggcgagcagc gcgtagcaat aggcggccag ggccagcgcg
     2221 ctaaccggca cgggcaagaa cagggtaaag ccgacgctga attgcgtggc gacgccctgc
     2281 aaccaggtct cgaacagcgg cgcgatcagc acgacagccc cggccagccc ggccagcacc
     2341 agccagagcg ggtggagccg gggcacgccg ggggcgcgcc ggaagagacc cgccgtccac
     2401 agcaggctaa tgcccgcggt catggcggcg gcttccccgg cggcgttggc ggccacgccg
     2461 aactcgccca ccggcagccc gaagccctgg gccaggatgg cggccagcgc gtacaggtag
     2521 tagcccaggt aggccaggcc gaacagggcc acggtgacgc gcagggccac cgtcccggtg
     2581 cgccgcagca gaaaggccgc cgcgatgccc aggatcacca ccgctgagat cagattatag
     2641 agaaacagca acggcagcgg cgtggccggg aagaggaagg ccacgctgaa cacgagcagg
     2701 acgatcagcg cggcggccag gaagcgcggc a
//
LOCUS       DASUYC010000123         4414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_380238,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000123 DASUYC010000000
VERSION     DASUYC010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4414
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380238"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /locus_tag="VF276_02000"
     CDS             <1..1161
                     /locus_tag="VF276_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605663.1"
                     /translation="ATNRQALSLLRRFYGRLALVVGVIGAAVVFSPLYDLLLRGVMNI
                     PADVAAAAQPTLAVFLLWPLPIGWRRLHQGALIRHGQSRAVGAGAFARIFALVAVIGA
                     LMPTLGGVINGSTIAALAMLASVTAESLYTHWSAGRLLADLPPAAPDGRHLTMRELWT
                     FYWPLAGTSILNTLNRPILSAGLAAAAVALPTGLDADAALAAWGVAWGILFLVNGATL
                     TLGQVAIAWDADARAAVRRRSERIIVGLGLGLSGVVALAVLSPLAAWLLDTVYVVTPA
                     IRAAALPVLALLVPLPLLMAADGLMRGKLIHRRQTRLVQRAQVIDLAVLAVVLLLGVY
                     WLVPAWQMPATTLGALAMLIVNATDAVILGLGVRANPRALALGPGVPAGQTP"
     gene            complement(1149..1529)
                     /locus_tag="VF276_02005"
     CDS             complement(1149..1529)
                     /locus_tag="VF276_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6605664.1"
                     /translation="MNNGKFILIVEDDPALRTSLTRDLEAQGYMVLQAGTYREALDML
                     TVRPALAIVDVTGPACAGWDVARWLEQTTDSVPLMLISDTLPDARRLQRYHPVACMPR
                     FCATTDLPAAVRSRLARPTTHYGV"
     gene            1851..3521
                     /locus_tag="VF276_02010"
     CDS             1851..3521
                     /locus_tag="VF276_02010"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6605665.1"
                     /translation="MSQRPGDRIARRPRPGPGATPARPRPRRAIALPAPDAEASPGSR
                     LARYSITVLVLLLVTALLAPVREIVDLLNIGLIYLLIVIAATLLAGRRTGILAAVLGF
                     ALFDFFLVPPYLTFAIDNLRNILALFVFLGISTLLSWLIAGAREQARQALRRAEDVSR
                     LYELNQAITGAQRRDEVLPAIAGKVAGVFEAQACWIFLPGPDRRLEVAALAPAGARAP
                     TRDELTLAGWAFRHGREVAQGDHDPAQPDSPAGRNRSTFLPLQGGRRTIGVLGVADKH
                     DQRPFTLAERTALATFAGQAAAALERIDLLRDAQRAELLARTDELKSALMSAVSHDLR
                     TPLASIMASATSLLEPGIAWDEATRRDLLESINEEAVRLNRLVGNLLDISRIEGGALR
                     PEKDWYAVGEVIDAVVGRLESRLGGHPLTVDVAPDIPLVPLDFTEIDQVISNLLENAL
                     KYTPPGTPIQIQARRAGDEVEIVVADRGPGVPPEHLAHLFDKFYRTGSVRPAHGMGLG
                     LAISKGLVEAHGGEIRAASTSGAGLTVTFTLPIPARAPGAPALVPTQP"
     gene            3625..4323
                     /locus_tag="VF276_02015"
     CDS             3625..4323
                     /locus_tag="VF276_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6605666.1"
                     /translation="MRELRILIVDDEPAIARALRPALQAQGFAVSIAATGGEALAGLE
                     SASPDLILLDLGLPDVDGVTLLQQIRARLHVPVIVLSVRGADRDKISALDSGADDYLT
                     KPFSMGELLARIRVALRHVQQAPPTATPRAPITIGAVVLDPEQHTVTAHGRPVHLSPT
                     EFNLLEILMTNAGKVSTHRTLLHKVWGPEYVSETQLLRVYISQLRGKIEDRPDRPAYI
                     LTEPGIGYRFRDSE"
BASE COUNT          638 a         1545 c         1560 g          671 t
ORIGIN      
        1 gcgactaacc ggcaggcgct gagcctgttg cggcggttct atggccggct ggcgctggtg
       61 gtcggggtga tcggggcagc ggtggtcttt tcgccgctct atgacctgct gctgcgcggc
      121 gtcatgaaca tcccggcgga cgtggccgcg gcggcacaac ccacgctggc cgtcttcctg
      181 ctctggccgc tgcccatcgg ctggcggcgg ctgcaccagg gcgcgctgat ccgccacggc
      241 cagtcgcggg cggtgggcgc gggcgcgttc gcccgcatct tcgccctggt cgccgtgatc
      301 ggcgcgctga tgcccacgct gggcggcgtg atcaacggca gcaccatcgc cgccctggcg
      361 atgctggcga gcgtcactgc cgagtcgctc tacacccact ggtcggcggg gcggctactg
      421 gccgaccttc cgcccgccgc gcccgacggt cgccacctga ccatgcgcga actctggacg
      481 ttctactggc cgctggccgg cacatcgatc ctgaacacgc tgaaccggcc catcttgagc
      541 gccgggctgg cggccgccgc cgtcgccctg cccacggggc tggacgccga cgccgcgctg
      601 gccgcctggg gcgtggcctg gggcatcctc ttcctggtca acggcgcgac gctgacgctg
      661 ggccaggtgg cgattgcctg ggacgccgat gcgcgggccg ccgtgcgccg ccgcagcgaa
      721 cggatcatcg tcgggctggg tttgggcctg tcgggcgtgg tggcgctggc cgtgctcagc
      781 ccgctggccg cctggctgct cgacaccgtg tacgtcgtaa ccccggcgat ccgggcggcg
      841 gccctgccgg tgctggccct gctggtgcct ctgccgctgc tgatggcggc ggacgggctg
      901 atgcgcggca agctgatcca ccgccgccag acccggctgg tgcagcgcgc ccaggtcatc
      961 gatctggccg tgctggcggt ggtgctgttg ctgggggtgt actggctggt gccggcgtgg
     1021 cagatgccgg cgaccacgct cggcgccctg gcgatgctga tcgtgaacgc caccgatgcg
     1081 gtcatcctgg gcctgggggt gcgcgccaat ccgcgcgccc tggccctggg gccgggcgtg
     1141 cccgccggtc agacgccgta gtgcgtggtg ggccgcgcca ggcggctccg gaccgccgcc
     1201 ggcaggtcgg tcgtcgcgca gaagcgcggc atgcaggcca ccgggtggta gcgttgcagc
     1261 cgcctggcgt cgggcagggt gtccgaaatc agcatcaagg gtacggagtc ggtcgtctgt
     1321 tccagccagc gggccacgtc ccacccggcg cacgccgggc cggtcacgtc cacgattgcc
     1381 agcgcggggc gcacggtgag catgtccagc gcctcgcggt aggtccccgc ctgtaacacc
     1441 atatagccct gggcttccag gtcgcgggtc aggctggtcc gcaacgccgg gtcgtcttct
     1501 acgatcagga taaacttgcc gttattcatt agtcgaatcc tctcggtttc cggcggcccg
     1561 gtagcacctt tgcgggatgc gggatcgaac aacaacccgc aatctgccgg cttctaccct
     1621 ggccggccgc ctctcacttg attctaagcc cgcgcccata agaaatagat aaagaaacag
     1681 cgccgggagc taagagaaag ataagcgcca ggcagcgggg gcataagaaa aggataagga
     1741 ccgcaacccc ggctcgcctc cgccggatac ggaatggcgc gccctcgcgg ctggatgtat
     1801 gataatgagg agattgccgt gtgctgcgtg cgaaggaggc ggagaggcgg atgagccagc
     1861 gtccaggcga ccgtatcgcg cgccgcccgc gaccagggcc gggggcgacc ccggcgcggc
     1921 cccgcccgcg ccgggccatc gcgctgcccg cgcccgacgc cgaggcctcg ccggggtcgc
     1981 ggctggcgcg ctacagcatc acggtgctgg tgttgctgct ggtgacggcg ctgctggccc
     2041 cggtgcgcga aatcgtcgac ctgctgaaca tcggcctgat ctacctgctg atcgtcatcg
     2101 cggcgacgtt gctggccggg cggcgtacgg gcatcctggc cgcggtgctc ggcttcgccc
     2161 tgttcgactt cttcctggtc ccgccgtatc tcaccttcgc catcgacaac ctgcgcaata
     2221 tcctggcgct attcgtcttc ctgggcattt cgacgctgct aagctggctc atcgccgggg
     2281 cgcgcgagca agcccgccag gcgttgcgcc gcgccgagga tgtgtcgcgc ctgtacgagt
     2341 tgaaccaggc catcaccggg gcacagcgcc gggacgaggt gttgccggcc atcgccggca
     2401 aagtggccgg ggtgttcgag gcgcaagcct gctggatctt cctgccgggg cccgaccggc
     2461 ggctggaggt ggcggcgctg gccccggcgg gggcgcgcgc ccccacccgc gacgagctga
     2521 cgctggccgg gtgggccttc cgccacggca gggaggtggc ccagggcgac cacgacccgg
     2581 cccaaccgga ctcacccgcc gggcgcaacc gctccacctt cttgccgttg cagggtggcc
     2641 ggcgcaccat cggcgtgctg ggcgtggccg ataagcacga ccagcggccc ttcaccctgg
     2701 ccgaacggac ggcactcgcc acctttgccg ggcaggccgc cgccgcgctg gagcgcatcg
     2761 acctgctgcg cgatgcccag cgcgccgaac tgctggcgcg caccgatgaa ctgaagtcgg
     2821 cgctcatgtc ggcggtatca cacgatctgc gcacgccgct ggcctcgatc atggcctcgg
     2881 cgaccagcct gctggaaccg ggcatcgctt gggacgaggc gacccggcgc gacctgctgg
     2941 agagcatcaa cgaggaggcg gtgcgcctga accggttggt cggcaacctg ctggacatat
     3001 cgcgcatcga gggcggggcc ttgcgcccgg agaaggactg gtacgccgtg ggcgaggtga
     3061 tcgacgcggt ggtggggcgg ctggaatcgc ggctgggcgg gcacccgctc accgtggacg
     3121 tggcaccgga tatccccctc gtgccgctcg acttcaccga gatcgaccag gtgatcagca
     3181 acctgctgga gaacgcgcta aaatacacgc cgccgggcac gcccattcag atccaggccc
     3241 ggcgcgcggg cgacgaggtg gaaatcgtgg tcgccgaccg ggggccgggg gtgccgccgg
     3301 aacacctggc gcacctgttc gacaagttct accggacggg cagcgtgcgg ccggcgcacg
     3361 gcatgggtct gggcctggcg atcagcaagg ggctggtcga agcccacggc ggcgagattc
     3421 gcgcggcgag cacatccggc gccgggctga ccgtgacctt taccctaccg atcccggcgc
     3481 gggcaccggg cgccccggcg ctcgtgccca ctcagccctg atagtccact ggacgacagg
     3541 ccggtttgaa accggcccct acagcgtaga cggacgcagg ccggtttgaa accggcccct
     3601 acaagacaca atcagcgagg agccatgcgc gagttacgca tcctaattgt cgacgacgaa
     3661 ccggccatcg cccgcgcgtt gcggcccgcg ttgcaggcgc agggcttcgc ggtcagcatc
     3721 gccgcgaccg ggggcgaggc cctggccggc ctggagagcg catcgcccga cctgatcctg
     3781 ctggatctgg gcttgcccga cgtggacggc gtcacgctcc tgcagcagat ccgggcgcgg
     3841 ctgcatgtgc cggtcatcgt gctgtcggtg cgcggcgccg accgggataa aatcagcgcg
     3901 ctcgatagcg gcgccgacga ctacctgacc aagccgttca gcatgggcga gttgttggcg
     3961 cgcatccgcg tggcgttgcg ccatgtgcag caagcacccc ccaccgccac accccgcgcg
     4021 cccatcacca tcggcgcggt ggtgctcgac cccgaacagc acaccgtgac cgcgcacggc
     4081 cggccggtcc atctcagccc caccgaattc aacctgctgg agatcctgat gaccaacgcg
     4141 ggcaaggtga gcacgcatcg caccctgctc cacaaggtct gggggccgga gtacgtgagc
     4201 gagacgcagt tgctgcgcgt ctacatcagc caactgcgcg gcaaaatcga ggaccggccc
     4261 gaccgtcccg cctacatcct gaccgaaccc ggcatcggct accgcttccg cgacagcgag
     4321 tagcggcggc gctctcgctc gggccaatac ccaggccgcg caggcggcct tcgtagcgtt
     4381 agccggaggc ttgtggggta taccccagcc gggc
//
LOCUS       DASUYC010000124         2759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_999193,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000124 DASUYC010000000
VERSION     DASUYC010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999193"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="VF276_02020"
     CDS             <1..650
                     /locus_tag="VF276_02020"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX6605667.1"
                     /translation="GEEAAVLEERNRMAREIHDTLAQGFTGIVVQLEAAEDTLTAEPA
                     AAQAHLDRARALARDSLAEARRSVWALRPRALEGGDLAGALDRAAQALTAGTATRAAV
                     HVHGVPRPLPPDLAGHLLRIGQEALTNAVRHAAARQVAIDLTYDPAAVGLRVRDDGSG
                     FDPAAPAPAAGGGLGLPGMRERVAGLGGTLTLTSAPGAGTEVRVQVPVGAHKESA"
     gene            647..1282
                     /locus_tag="VF276_02025"
     CDS             647..1282
                     /locus_tag="VF276_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015181742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6605668.1"
                     /translation="MSKEGAIRVLIADDHPVVREGVAAMIERRPDMTVVGEAATGREA
                     VAAYRAAQPDVVLMDLRMPDMTGVEAIGAIRGEFPGARIIVLTTYDGDEDIYRGLQAG
                     ARAYLLKDAPRDDLLDAIRAVHAGQSRIPPAVAARLAERVLAGPELTARELEVLQGIV
                     AGRSNKEIGAALGISEGTVKAHVNSILGKLGVADRTQAVTTALQRGIVHLD"
     gene            1612..2313
                     /locus_tag="VF276_02030"
     CDS             1612..2313
                     /locus_tag="VF276_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6605669.1"
                     /translation="MHRILPGLYTFSGLGMGRVYLIRDPDGFTLIDASIRGAADKIVA
                     QIRRAGGSARDVRRILITHAHPDHVGGLPRLKELTGAEVICSEIERPVTEGTAPINRP
                     AGDQLRGPLGRARAAALLPGTPVDRVVGEGDTLPEVMGGLRVLAVPGHAPGHLAFWQP
                     ERGVLFCGDVMMRVAGLRLPIAPFTADMAENRRSITRLAALDTRVLCLGHGVPLTQNA
                     AATIRAFAVQEGRPL"
     gene            2310..2714
                     /locus_tag="VF276_02035"
     CDS             2310..2714
                     /locus_tag="VF276_02035"
                     /inference="COORDINATES: protein motif:HMM:NF020950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HEX6605670.1"
                     /translation="MSKRLRWGLLGGLVLLILVVGGAAALLLAGPPTYSDESVPITVA
                     PGGDFAIRLASNATTGYRWELHSAPDSAVVTLRESRYDGPDAPLVGAGGHEVWTFHAV
                     APGQTTIGLRYAFQDHAPTRPTQDTVFTVIVR"
BASE COUNT          374 a         1012 c         1016 g          357 t
ORIGIN      
        1 gcggcgagga ggccgcggtg ctggaggagc gcaaccgcat ggcccgcgaa atccacgaca
       61 cgctggccca gggcttcacc ggcatcgtgg tgcagttgga ggccgccgag gacacgctga
      121 ccgccgagcc cgccgccgcc caggcccacc tggaccgggc gcgcgccctg gcccgcgaca
      181 gcctggccga ggcccgccgc tcggtctggg ccttgcgccc gcgcgcgctc gaaggcggcg
      241 acctggccgg ggcgctcgac cgcgcggccc aggcgctgac cgcgggcacg gccacgcgcg
      301 ccgccgtcca cgtccacggc gtgccccgcc cgctgccgcc cgacctggcc ggccacctgc
      361 tgcgcatcgg ccaggaggcg ctgaccaacg ccgtgcgcca cgccgccgcc cgccaggtgg
      421 cgattgacct gacctatgat cccgccgccg tgggcctgcg cgtgcgcgac gacggcagcg
      481 gcttcgaccc cgccgcgccg gcgcccgccg ccgggggtgg cctgggcttg cccggtatgc
      541 gcgagcgcgt cgcgggcctg ggcggcacgc tgaccctgac cagcgcgccc ggcgccggga
      601 ccgaggtgcg cgtgcaggtg cccgtgggcg cgcacaagga gtccgcatga gcaaagaggg
      661 cgcgatccgc gtgttgatcg ccgacgacca cccggtggtg cgcgaagggg tggcggccat
      721 gatcgagcgc cgccccgaca tgaccgtggt gggcgaggcc gcgacgggcc gcgaagccgt
      781 ggccgcctat cgcgccgccc agcccgacgt ggtgttgatg gacctgcgca tgcccgacat
      841 gaccggcgtg gaggctatcg gcgccatccg cggcgagttc cccggcgcgc gcatcatcgt
      901 gctgaccacg tacgacggcg acgaggacat ttatcgcggg ctgcaagccg gggcgcgggc
      961 ctacctgctc aaggacgccc cacgcgacga cctgctcgat gccatccgcg ccgtccacgc
     1021 cgggcagagc cgcatcccgc ccgccgtggc cgcgcgcctg gccgagcgcg tgctggccgg
     1081 gccggaactg acggcgcgcg aactggaggt gttgcagggc atcgtcgcgg gccgcagcaa
     1141 caaggagatc ggcgcggcgc tgggcatcag cgagggcacg gtcaaggccc acgtcaacag
     1201 catcctgggc aagctgggcg tggccgaccg cacccaggcc gtgaccacgg ccctccagcg
     1261 cggcatcgtg catctggatt agccggcgcc ggggcgggcc gggccggttt caaaccggcc
     1321 cctacagccg cgattgaggc gggccggttt gaaaccggcc cctacagccg cgattggggg
     1381 acggccggtg gcagcccggc gtctgccgcg ggttgcgggc cggttggaaa ccggccccta
     1441 cagcgcgatt gaagtgggtc ggtggcagcc ctgcgtctgc cgcgggcgcg ctacaccttc
     1501 gcccttgtca ctctgagcgc agcgaagagt ctcgtcgccg ttggggtacg agacgagatt
     1561 cttcgctgcg ctcagaatga cagtaacagt agaagagaag acaggagatc tatgcaccgc
     1621 attctaccag ggttatacac tttcagcggc ctcggcatgg ggcgcgtgta cctaatccgc
     1681 gaccccgacg ggttcacgct gatcgacgcg agtattcgcg gggcggcgga caagatcgtg
     1741 gcgcagatcc ggcgggcggg cggcagtgcc cgcgacgtgc gccgcatcct gatcacccac
     1801 gcccacccgg accacgtcgg cgggctgccg cgcttgaagg agctgaccgg cgccgaggtg
     1861 atctgctcgg agatcgagcg gcccgtgacc gagggcacgg cgcccatcaa ccgcccggcg
     1921 ggcgaccagt tgcgcgggcc gctgggccgg gcgcgggcgg cggcgctgct gccgggcacg
     1981 ccggtggacc gcgtggttgg cgagggcgac acgctgcccg aggtcatggg cgggctgcgc
     2041 gtactggccg tgccggggca cgcgcccggc cacctggctt tctggcagcc ggagcgcggc
     2101 gtgctcttct gcggcgacgt gatgatgcgc gtggccgggc tgcgcctgcc catcgccccg
     2161 ttcaccgccg acatggccga gaatcgccgc tcgatcacca ggctggccgc gctcgacacg
     2221 cgggtgctct gcctgggcca cggcgtgccg ctcacgcaga acgcggcggc gaccatccgc
     2281 gcctttgccg tgcaggaggg ccgcccgcta tgagcaagcg gttacgctgg gggctgctgg
     2341 gcgggttggt cctgctgatc ctggtcgtgg gcggggccgc cgccctgctg ctggccgggc
     2401 cgcccactta cagcgacgaa tcggtgccga tcaccgtggc gccgggcggc gatttcgcca
     2461 tccgcctggc ctcgaacgcg accaccggct accgctggga actgcacagc gcgcctgaca
     2521 gcgcggtcgt cacgctgcgc gaatcgcgct atgacggtcc cgatgcgccc ctggtggggg
     2581 ccggcggcca cgaggtctgg accttccacg ccgtcgcgcc gggccagacg accatcggcc
     2641 tgcgctacgc cttccaggac cacgccccca cccgccccac ccaggacacc gtcttcacgg
     2701 tcatcgtccg ctgagcgcgg cgccggccct gtgtcactct gagcgcagcg aagagtctc
//
LOCUS       DASUYC010000125         3830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_760258,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000125 DASUYC010000000
VERSION     DASUYC010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760258"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VF276_02040"
     CDS             complement(<1..209)
                     /locus_tag="VF276_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX6605671.1"
                     /translation="MEPFEHVDARSVAGAITLLEGAEGGAAAARPIAGGTDLLTTLKV
                     GIHAPVRLVNLKTINGLDSISDFAGT"
     gene            complement(209..2536)
                     /locus_tag="VF276_02045"
     CDS             complement(209..2536)
                     /locus_tag="VF276_02045"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6605672.1"
                     /translation="MVRVVRTKVEIEGRTYEETVVVEGEEPQPWQQDELHYVGRETVR
                     IDGVERVTGAARYSADIQLPGMLYAAIARCPYAHARIRSIDVSAAEALPGVRYILTHQ
                     NAPPIPWHSRASRIFDTELRYEGDEVAAVAADDSATADRAVRLITVDYEPLPAVIDPE
                     AALRPDAPQVHPDGNVLKDDDGQPGETYTRGDLDHGFRDADVVVEGRYTTPAQMHNAL
                     ETHGCVAAWDGATLTVYESTQAIYEVRDGLARALGLRRSQVRVIKNYMGGGFGAKFGA
                     GKHTIIAALLARATGRPVHLMLDRRAENLVAGNRAPTIQYIKIGARKDGTLTALDLRI
                     ISQLGAYGNWTPAIAGPAKEMYEIPNLRVQVFGVRTNTGTHAAFRAPGYVEGTVALDG
                     ALDDLARKLNMDPLALRRKNYTQKDPPTGQDYTAKHLDECYTRGAAMFGWDDLRRQLA
                     AQRAADGAPPTMRRGIGMATQTWGGGGGPPAQALCRINGDGSVDVYCGTQDLGTGTRT
                     VLAQIAADALGFPLEQIRVHLGDTEVGPYGPGSGGSATVASAGPAMRMAAADARKQLL
                     EIASAFMDTSPDKLEVREGNIVRTNNPKESMAIKDVLDQIGDYQITGKGFRGANPAQP
                     IRTWGAQFAEVLVDTVTGRVQVVRTVGVYDTGRVINPLTYRNQIHGGIVQGLGLALTE
                     ERILDPPTGKSLNPNLEDYKVPTIADIPVIDVDWIGEPDLAANHVGAKGIGEPPIIPT
                     PAAIANAVADALGVRVFDLPITPERVLTALAGGKP"
     gene            complement(2540..3064)
                     /locus_tag="VF276_02050"
     CDS             complement(2540..3064)
                     /locus_tag="VF276_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015656432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX6605673.1"
                     /translation="MTNSTGTGPAGAVRGPGSTEIHLHVNGQPYTIQAPPARTLLDAL
                     REDLGLTGTKNVCDQGQCGACTVLRDGVAVYACLTLAVECGRNEITTIEGLAAPDGTL
                     HPVQQAFLDCDDLQCGFCTPGQVLAMKALLDRHPNPTPDDIARGMSGNLCRCGAYPGI
                     RRAAEQAARALQGA"
BASE COUNT          490 a         1255 c         1434 g          651 t
ORIGIN      
        1 gtcccggcga aatcgctgat gctgtcgagg ccgttgatcg tcttgagatt gaccaggcgc
       61 accggcgcgt ggatgcccac cttgagcgtg gtcagcaggt cggtgccccc ggcgatgggc
      121 cgggccgcgg cggcgccgcc ctccgcgcct tcgagcaggg tgatggcgcc ggcgaccgac
      181 cgcgcatcga catgttcaaa cggttccatc agggctttcc tccggcgagc gcggtcagca
      241 cccgctccgg cgtaatgggc agatcgaaga cgcgcacgcc gagcgcgtcg gccacggcgt
      301 tggcgatggc ggcgggcgtg gggatgatcg gcggctcgcc gatgcccttg gcgcccacat
      361 ggttcgcggc caggtcgggc tcgccgatcc agtctacgtc gatgaccggg atatcggcga
      421 tggtcggcac cttgtagtct tcgagattgg ggttgagcga cttgccggtg ggcgggtcga
      481 ggatgcgctc ctcggtcagc gccaggccca ggccctgcac gatgccgccg tggatctggt
      541 tgcgataggt cagcgggttg atcacgcggc ccgtgtcgta gacgcccacg gtgcgcacga
      601 cctggacccg cccggtgacg gtatcgacca gcacctcggc gaactgcgcg ccccaggtgc
      661 ggatcggctg ggcggggttg gcgccgcgga agcccttgcc ggtgatctgg tagtcgccga
      721 tctggtcgag cacgtccttg atcgccatgc tctctttcgg gttgttcgtg cgcacgatgt
      781 tgccctcgcg cacctccagc ttatcgggcg aggtgtccat gaaggccgag gcgatttcga
      841 gcagttgctt gcgcgcgtcg gcggcggcca tgcgcatggc aggaccggcg gacgccaccg
      901 tggccgaacc gccgctgccc ggcccgtagg ggccgacctc ggtgtcgccc aggtggacgc
      961 ggatctgctc cagggggaag cccagcgcgt cggcggcgat ctgggccagc acggtgcgcg
     1021 tgccggtgcc gagatcctgg gtgccgcagt agacatcgac gctgccgtcg ccgttgatgc
     1081 ggcaaagggc ctgcgcgggc gggccgccgc cgccgcccca ggtctgcgtc gccatgccga
     1141 tgccgcggcg catggtgggc ggcgcgccgt ccgcggctcg ctgcgcggct aactggcggc
     1201 gcagatcgtc ccagccgaac atggccgcgc cgcgcgtgta gcactcgtcg aggtgcttgg
     1261 cggtgtagtc ctggccggtg ggcgggtcct tctgggtgta gttcttgcgg cgcagggcga
     1321 gcgggtccat atttagcttg cgcgccaggt cgtccagcgc gccgtcgagc gccacggtgc
     1381 cttcgacgta gccgggggcg cggaaggcgg cgtgggtgcc ggtgttggtg cgcacgccga
     1441 agacctgcac gcgcaggttg gggatctcgt acatttcctt ggccggtccg gcgatggcgg
     1501 gcgtccagtt gccataggcg ccaagctggc tgatgatgcg cagatcgagg gccgtgagcg
     1561 tgccgtcctt gcgcgcgccg atcttgatgt actggatcgt gggcgcgcgg ttgccggcga
     1621 cgaggttctc ggcccggcgg tcgagcatga ggtggacggg gcggcccgtg gcgcgggcca
     1681 gcagggcggc gatgatcgta tgcttgcccg cgccgaactt ggcgccgaac ccaccgccca
     1741 tgtagttctt gatcacccgc acctggctgc ggcgcaaccc cagcgcgcgg gccagcccgt
     1801 cgcgcacctc gtagatggcc tgggtgcttt cgtagaccgt cagggtggcg ccgtcccagg
     1861 cggcgacgca gccgtgggtc tccagcgcgt tatgcatctg ggcgggggtg gtgtagcggc
     1921 cctccacgac cacgtcggcg tcgcgaaagc cgtggtccag gtcgccgcgg gtataggtct
     1981 cgcccggctg cccgtcgtcg tccttgagca cgttgccgtc tgggtggacc tggggcgcgt
     2041 cggggcgcag ggccgcctcc gggtcgatca cggcgggcaa gggctcgtag tccaccgtga
     2101 tcaggcgcac ggcacggtcc gccgtcgcgg agtcgtcggc ggccacggcg gccacttcgt
     2161 cgccttcgta gcgcagttcg gtgtcgaaga tgcgcgaggc gcgcgagtgc caggggatgg
     2221 gcggcgcgtt ctggtgggtc aggatgtagc ggacgccggg cagcgcctcg gcggcgctca
     2281 cgtcgatgct gcggatgcgg gcgtgggcat acgggcagcg ggcgatggcg gcgtagagca
     2341 tgcccggcaa ctggatgtcg gcggaatagc gggccgcgcc ggtgacgcgc tccacgccgt
     2401 cgatgcgcac ggtttcccgg cccacatagt ggagttcgtc ctgttgccag ggctggggct
     2461 cctcgccctc gacgaccacc gtttcctcgt aggtgcggcc ctcgatttcg actttggtgc
     2521 gaaccacacg taccatggct taggctccct gcagggcacg cgccgcctgc tccgccgcgc
     2581 gccggatgcc cggataggcg ccgcagcggc agagattgcc ggacatgccg cgcgcgatgt
     2641 cgtccggggt ggggttgggg tggcggtcga gcagcgcctt catcgccagc acctggccgg
     2701 gcgtgcagaa gccgcattgc aggtcgtcgc aatcgaggaa cgcctgctgg acggggtgca
     2761 gcgtgccgtc gggcgcggcc aggccctcga tggtggtgat ctcgttgcgc ccgcattcga
     2821 cggccagcgt caggcaggcg tagacggcga cgccgtcgcg cagcaccgtg caggcgccgc
     2881 actggccctg gtcacagacg ttcttcgtgc cggtcaggcc caggtcttcg cgcagcgcgt
     2941 cgagcagcgt gcgcgcgggc ggcgcctgga tggtgtaggg ctggccgttg acgtgcaggt
     3001 ggatttcggt ggaaccgggc ccgcggacgg cgcccgccgg gccggtccct gtgctgttgg
     3061 tcataccgtg tgctcctgtc gtgggctggg ggatttgacg ccgcggccgc cccgcctgga
     3121 tggggccggg ccgccggaac attccgcggc ataccagcaa gtttcgcgcc ctgacgccgg
     3181 atcgcgcaac cttttcgcga tttgcacagt atacatagta gagagaccgc aaggtacccc
     3241 acccaggtgc ctgggcgacc ccccgcgaag tctgccccgg cgggcttcgc ggatgggcca
     3301 ggcggatagc cgtcagccgt cagtggtcag tggtcagtgg tcagtggtca gtggtcagtg
     3361 gtcagtggtc agtgaccggc gtccgacccg gcaacgggca gtctcgcccg gcccgtgaac
     3421 gggcgggcta gcgctacgaa gcccgccttc gcgggctatt ccattcgcaa tccggccgtc
     3481 cgcgcggtag cgagatcgcc cggcccgtga acgggcgggc tagcgctacg aagcccgcct
     3541 gcgcgggcta tttccccgga gacaggccgc gcaggcggcc ttggttttcg tagcccgcgg
     3601 cttgagccac cgggcggccc cccgctaacg tgcatcctcc aatattgagc cctcgtctcc
     3661 tgacggctga cggctgacgg ctgacagcta tccgttgttg accccgccgg gccttttggc
     3721 atataatagc ctcgccttat ttgcgccccg atgcgcgcag tttccgtcag atagcgacgc
     3781 ggctggaccg ggaatcgccg gatgccggcc tgcgcgggcg gcaccacgac
//
LOCUS       DASUYC010000126         4601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1221217, whole genome shotgun sequence.
ACCESSION   DASUYC010000126 DASUYC010000000
VERSION     DASUYC010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4601
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221217"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(273..587)
                     /locus_tag="VF276_02055"
     CDS             complement(273..587)
                     /locus_tag="VF276_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012813798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605674.1"
                     /translation="MTTLLTRLTLLVVGVVVSVLAIYLILIAVALIRANQNLEKLVGG
                     LTAIRDNTAPLKEDLATINGAAVVLRNRLVSVDTHLQGIARHLPGTASGSPTGEHREA
                     HG"
     gene            complement(584..754)
                     /locus_tag="VF276_02060"
     CDS             complement(584..754)
                     /locus_tag="VF276_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605675.1"
                     /translation="MAENTANVAALKDTIAVAPQLLAGAEALDRHATIIETALAAAPP
                     GDGHVAREEVGP"
     gene            complement(903..1166)
                     /locus_tag="VF276_02065"
     CDS             complement(903..1166)
                     /locus_tag="VF276_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008865784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605676.1"
                     /translation="MLTFVGALVAFLARITKALEAIGGQADSSLAKLAFGLRAIETET
                     SHLAPQVTQLNAGLTSLGGKLGVVDGHLQAVAEALTGGKGAGG"
     gene            complement(1236..1550)
                     /locus_tag="VF276_02070"
     CDS             complement(1236..1550)
                     /locus_tag="VF276_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605677.1"
                     /translation="MKTRKPQGVPEATVVRYWSTALVAGLAVTGVVAVLLTILARTAE
                     AIEAGAAEIWRVGKLIANNTVHIPLLTRTNQQVAAILETADGIAAATARIGAAVTGKP
                     EL"
     gene            complement(1547..1858)
                     /locus_tag="VF276_02075"
     CDS             complement(1547..1858)
                     /locus_tag="VF276_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605678.1"
                     /translation="MSEKDFHRYWFAGGFFATAVVAVVATLVLAIIGTARSILANAGR
                     ALGLANEIVTTTAPIWELDTTNAVAGQLLEGAQAIRQHATQVADALAGPAPGTAGEPR
                     P"
     gene            complement(1881..3218)
                     /locus_tag="VF276_02080"
     CDS             complement(1881..3218)
                     /locus_tag="VF276_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010548666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression protein HypE"
                     /protein_id="HEX6605679.1"
                     /translation="MPRSPAAEATVAKRVEPVDWLTSSAERELHKDPAAGRQGALLGP
                     VKVAHAFWLAGMSCDGCTVSVSGATAPSVEDLLTGRMPGVPRVVLHHSVLSAEAGHEF
                     IKNYQMAVDGKLNAPYVVIYEGSITDERLAAATGGYWSAMGVEEEDGQQKPIPTATWL
                     KRMAPGAAAVIAIGTCATWGGIPAAYGNPTGAMSVMDFLGKDYRSALGLPVVNVPGCA
                     PVGDNFTETVAAILLFLQGLGPLPEFDERGRPAWLFSETVHQRCVRAGYYEEGTFAEE
                     YGDKECLVEIGCWGPVVQCNITSRGAINHMGGCMNTGGGCIGCTMPGFPDRFAPFYNR
                     APGALVSTNSSRVVGSFVRPLRRMTQQFQNRETRWDRDGEHVPSGWGNVAAPGPIKKG
                     MHFFYEKLQHQGSVTPGRTRPAEQYRNGYEVPGVRSGESARPERREQTGGNGR"
     gene            complement(3274..3726)
                     /locus_tag="VF276_02085"
     CDS             complement(3274..3726)
                     /locus_tag="VF276_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605680.1"
                     /translation="MLGQLIAETDALTIVDMEASVEHMGRGTVRYADILLVLAEPYYR
                     SLETVGRMAPLAQDLGIGRVALVANKVRTPRDEEAIRRYCAERDLAVIAVLPFDDVVL
                     EADQAGIPILDRAADAPSVQVVAELQQRIESAAASVPALDRGDSTAVG"
     gene            complement(4414..>4601)
                     /locus_tag="VF276_02090"
     CDS             complement(4414..>4601)
                     /locus_tag="VF276_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011140052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEX6605681.1"
                     /translation="LDAQRASPARRRLRAIEGQVRGLVPMIDEDRPCLDMLVQIAAAQ
                     EALSQVNKLVMRTFLEN"
BASE COUNT          752 a         1593 c         1461 g          795 t
ORIGIN      
        1 cgaccagcgc agcgctgcca actgagcagc cgacccacca tgccatgctt gtcgtttggg
       61 gccaattcgc ccaggaactc ggtttcctgg cgcaactcgc ccaggtcccc atcccccaga
      121 aaaccgtcca gcacccgccc gccgccaaat tagcgacctt gttcatgggc ttgctctcgg
      181 gcatggagta cctaaccgat ctcacgcggg cccccgcgcc gctgtcgccc tcgctccatg
      241 acccggcgcc gggcgccgga atccgctgcg cgctagccgt gcgcctcccg gtgttcaccg
      301 gtggggctgc cgctggcggt gcccggcaaa tgccgggcaa tgccctgcaa atgggtgtcc
      361 acactcacca gccggttgcg taggaccacg gcggccccgt tgatggtcgc caagtcctct
      421 ttcagcggtg cggtgttgtc ccggatcgcc gtcaggccgc ccaccaattt ctccaggttc
      481 tgattggccc gaatgagcgc gacggcgatc aaaatcaggt agatcgccag caccgacacc
      541 accacgccga cgaccagtag cgtcaggcgg gttaagaggg tcgtcatggg ccgacctcct
      601 cgcgggcgac atgcccgtca ccgggcggcg ccgcggccag ggccgtctcg atgatcgtcg
      661 cgtgccgatc gagcgcctcg gcgccggcga gtagttgggg cgcgaccgcg atggtgtcct
      721 tcagggccgc cacgttggcc gtattttcgg ccaccccgac gcctgccgcc agcactccgc
      781 ggtgtattgc tcaatatggc gggccgcggc gagcagccgc gtcagatagc ggaccacgat
      841 gggcacaacg ccgaagacgg tagcgaggag cataccggcc cagatggcaa ggatcccccg
      901 gttcatcccc cagccccctt tccacccgtg agcgcctcgg ccacggcctg caagtggccg
      961 tcgaccacgc cgagcttgcc gcccaggctg gtcagccccg cattgagttg ggtcacctgc
     1021 ggcgcgaggt ggctggtttc cgtctcgatc gcccgcaagc cgaaggcgag cttggcgagc
     1081 gagctatccg cctggccgcc gatggcttcc agcgctttgg tgatgcgcgc caggaaggcg
     1141 accaacgcgc ccacaaaggt cagcacctcc agggcagaga ggatcgtaag tatcagggcg
     1201 atcaggcact ctctccttac ggcacggggc cgtggctaaa gctccggctt gccggtcacc
     1261 gccgcgccaa tgcgcgccgt ggcggcggca atgccatcgg cggtttccag aatggcggcg
     1321 acctgttggt tggtccgcgt gagcagcggg atatggacgg tgttgttggc gatgagcttg
     1381 ccgacgcgcc agatctcggc ggccccagcc tcgatcgcct ccgccgtgcg cgccaggatg
     1441 gtgagcagca cggcgacaac gccggtcacg gccagccccg ccacgagggc cgtactccag
     1501 tagcggacga cggtggcttc cgggactcct tgcggcttgc gtgttttcat ggccgcggct
     1561 ctcccgcggt gccaggcgcc gggccggcca gggcatccgc cacctgcgtg gcatgttgcc
     1621 ggatcgcctg cgccccttcc agcaactggc ccgccacggc gttggtcgtg tccagttccc
     1681 agatcggtgc ggtggtcgtc acgatctcgt tggctaagcc gagcgcccgc ccggcattag
     1741 cgaggatact ccgcgccgtg ccgatgatcg ccagcaccaa ggtggcgacc actgcgacca
     1801 ccgccgtggc gaagaatcca ccggcaaacc agtagcgatg gaagtccttt tcgctcatgc
     1861 cgcctcctct accgtcgccg tcagcgcccg ttgccgccgg tctgttcgcg ccgctccggt
     1921 cgggcgctct cacccgaccg cacgccaggc acctcataac cattgcggta ttgttccgcg
     1981 ggtcgggtcc ggcctggcgt tacgctgccc tggtgctgga gcttttcgta gaagaaatgc
     2041 atccccttct tgatcggccc tggtgcggcc acgttccccc agccgctcgg tacgtgctcc
     2101 ccgtcgcgat cccagcgcgt ctcgcggttc tggaactgct gggtcatgcg gcgcagcggc
     2161 cggacgaacg agccgaccac acgcgacgag ttggtggaaa ccagcgcgcc cggcgcacgg
     2221 ttgtagaacg gggcgaaccg atccgggaag cccggcatgg tgcagccgat gcagccgccg
     2281 cccgtgttca tgcagccgcc catgtgattg atcgcgccgc gcgaggtgat attgcactgc
     2341 acgacgggac cccagcagcc gatctccacc aggcattcct tgtcgccgta ttcctcggcg
     2401 aaggtgccct cctcgtagta cccggcccgc acgcagcgct ggtggaccgt ttcgctgaac
     2461 agccaggccg ggcggccgcg ttcatcgaac tcgggcaacg ggcccagccc ttgcaggaac
     2521 agcaagatcg cggcaaccgt ctccgtgaaa ttatcgccaa cgggagcgca gcccggcacg
     2581 ttgactaccg gcaagcccag tgcgctacgg taatccttgc cgagaaagtc catcacactc
     2641 atcgccccgg tggggttgcc gtaggccgcg ggaatgccgc cccaggtggc gcaggtgccg
     2701 atggcgatca ccgcggcggc gccgggcgcc atgcgcttca gccaggtcgc cgtcgggatc
     2761 ggtttctgct gcccgtcttc ctcttccacg cccatcgccg accagtagcc cccggtcgcg
     2821 gcggcgaggc gctcatcggt gatcgagccc tcgtagatca cgacataggg ggcgttgagc
     2881 ttaccgtcga cggccatctg gtagttcttg atgaactcgt ggccggcttc ggccgacagc
     2941 accgagtggt gcagcacgac tcgcggcacg cccggcatgc gcccggtcag caggtcttcg
     3001 acgctcgggg ccgtcgcgcc cgacacggat acggtacatc cgtcacagct catgcccgcc
     3061 agccagaagg cgtgcgcgac tttgaccggc cccagcagcg ctccctggcg gcccgcggcc
     3121 gggtctttat gcaactcccg ctccgcactc gatgttagcc agtcaaccgg ttccacgcgc
     3181 ttcgccactg ttgcctcggc ggcgggcgat cgcggcattg gcttctgttc catagcaacg
     3241 tgcctttcct aagaccgtgg aatggcgccg tggttatcct acagccgtgc tgtcaccccg
     3301 atcaagagcg gggacgctgg ccgccgccga ctcgatgcgc tgctgtaatt cggcaactac
     3361 ctgtacagac ggcgcgtccg cggcgcgatc cagtataggg atgcctgctt ggtcggcttc
     3421 cagcacgacg tcgtcgaacg gcagcacggc gatcaccgct aaatcgcgtt cggcgcagta
     3481 ccgccggatc gcctcttcat cgcgaggtgt ccgcaccttg tttgccacaa gcgcgacccg
     3541 gccgataccc aggtcctgcg ccagcggcgc catgcgcccc actgtctcca gagaccggta
     3601 gtagggttcg gccagcacga gcaagatatc ggcatagcgc actgtgcccc gccccatatg
     3661 ctcgaccgag gcctccatat cgacaatggt cagggcgtcc gtctcggcga tcaactggcc
     3721 taacatgcca cgcacggtgg catgcgcgcc acacagtcag ccggcgcccg cgtggtccac
     3781 ctgtgcggtc agcaacagcc ggacgccgtc cggggcgacc aggccgtgcg tcgcggcgat
     3841 ttcctctacg ggagcggtga gagtcgtgcg ggttccgccg tcagaagctt tcgtttcctg
     3901 gagaatggtg cgtggcagcg ggtggagcgc cgtgaggctg gtctgcgcca ggcccagggc
     3961 aaccccgagg ttcgggttgg gatcgccgtc gatagccagg accgattatc cgtcccgtgc
     4021 aaagagccgg gccagcgcgg cagcgagtgt ggttttcccc gcaccgccct tgccggcaat
     4081 ggcgagtttc atcgagggtg gcctccttca acggagcgga aaacaacgcc tctttaccgg
     4141 attatagcag aggccgggcc gctgtcaaaa cgtgggtccg atccggcaca ttttcagcgc
     4201 aatataagcc tgatatggcg gccacggagg attagcggtg ggcacgcggc ccaataaccg
     4261 ccggaaatag aggtagcaga ccccccctgg ggtcttgtaa cgactagcat ccgatagcag
     4321 cagtcaccgc cgatatatat aaataacatc caggagatcg tcgtagacga tggcctgctc
     4381 ctccgcgctg gtcgcctgca gggccgtgcc cgcctagttt tccaggaagg tgcgcatgac
     4441 caatttgtta acctgcgaca aggcttcttg cgcggcggcg atttgcacga gcatatccag
     4501 acagggccga tcttcgtcaa tcatcggcac cagaccacgc acttgccctt caatggcccg
     4561 cagtcgccgg cgcgcggggc tcgcacgttg ggcatcgagt t
//
LOCUS       DASUYC010000127         2129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_561819,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000127 DASUYC010000000
VERSION     DASUYC010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561819"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2129
                     /locus_tag="VF276_02095"
     CDS             <1..>2129
                     /locus_tag="VF276_02095"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX6605682.1"
                     /translation="LDRYVAVKVLYPFLLNEEDSRDRFRKEARAVAALRHTNIVQVID
                     YDDEGDVYFMVMEFIDGPNLKEVLHREHVAGTRLSLAQIEQVITAIGNALSYAHKHGM
                     IHRDIKPHNIMFTAEGHPLLTDFGIAKIVSAATSSASGGLSGTPAYMSPEQGRGAKLD
                     PRTDIYSLGVVLYEMVTGRVPFDADTPFAVVIKHMNEPLPLPHLVDSSVPEAVERVVL
                     KAMAKAPEDRYQTAEEMVRALQQAVAEAQAGQPAVTAGPPLVVTPAVAANAPTLDTTP
                     AMLPAEVGTPGLATAPPAEPATATSAAATLPGAATIPAPADGEVPANLPPAAALEVAQ
                     ATSPTQGMPPALAPVELAATLPAEVVAADAAATVSPALVTEAAASEVAQTIPPTAGTA
                     DEVAQTISPAPAPEATAGEVAQTISPTAAGAAGEVAQTIPSGPAPVDAAPEVAQTLPS
                     APREPAAAPTIPPITPAPLVAETAPPAAPMAGVSAPPPSAWPPDAEYPVPSMVSAPVP
                     TTPGVPAPVYEAPVAVPPAPGYQAPVAVPPAPAYQAPVAVPPMPPRVERGAPAARPAG
                     RSGRTLAFAAGGLLIALLLLAGGLAALAGNKPQPPPAPTPTVLAANTGATATAAAAGA
                     ADATGTAQGALAQSAATGTAAAQGAAQATGTVLAQATAAAQSQAAGQATATAQFEAAA
                     QATAAAQTQDAQAQATAAAQAQAAAAE"
BASE COUNT          318 a          822 c          745 g          244 t
ORIGIN      
        1 cctcgaccgc tatgtcgccg tgaaggtgct ctatcccttc ctgctcaacg aagaggacag
       61 ccgcgaccgc ttccgcaaag aagcgcgcgc cgtcgccgcg ctgcgccaca ccaacatcgt
      121 ccaggtgatc gactatgacg atgagggcga tgtctacttc atggtgatgg agttcatcga
      181 cgggccgaac ctcaaggaag tgctgcaccg cgagcacgtc gcgggcaccc ggctgagcct
      241 ggcccagatt gagcaggtga tcacggccat cggcaacgcg ctgtcgtacg cgcacaagca
      301 cggcatgatc caccgcgaca tcaagccgca caacatcatg ttcaccgccg aaggccaccc
      361 gctgttgacc gatttcggca tcgccaagat cgtcagcgcg gccacgtcga gcgccagcgg
      421 cgggctgtca ggcacgcccg cctacatgag cccggagcag ggccgcggcg cgaaactcga
      481 cccgcgcacc gatatctact cgctgggcgt cgtgctctac gagatggtca ccgggcgcgt
      541 gccgttcgac gcggatactc cctttgccgt ggttatcaag cacatgaacg agccgttgcc
      601 cctgccccat ctggtggaca gcagcgtgcc cgaagccgtc gagcgggtgg tgttgaaggc
      661 gatggcgaag gcgccggagg accgctacca gacggcggag gagatggtgc gggcgttgca
      721 gcaggcggtc gccgaagcgc aggccggcca acccgctgtg acggctggcc ccccgctggt
      781 ggtcacgccc gccgtcgcgg ccaacgcccc gacgctggac acgaccccgg ccatgctgcc
      841 tgcggaagtg ggcacacccg gcctggcgac ggccccgccc gccgaaccgg caaccgcgac
      901 ctctgccgcc gccacgctgc ccggcgcggc gacgatcccc gcgcccgcgg acggggaagt
      961 gccggcaaac ctgccgcctg ctgcggcgct ggaggtcgcc caggcgacga gtcccaccca
     1021 ggggatgccg cctgctctgg cccccgtgga actggccgcc accctgccgg cagaggtggt
     1081 cgccgccgat gcggcagcca ccgtgtcgcc cgccctggtg acggaagccg cggcgagtga
     1141 agtcgcgcag accatcccgc ctaccgccgg cacagccgat gaggtggcgc agaccatctc
     1201 gcctgccccg gcgccggagg ccaccgcagg ggaggtcgcg cagaccatct cgcccaccgc
     1261 agccggcgcc gcaggcgagg tcgcacagac catcccgtcc ggcccggcac cagttgacgc
     1321 ggcaccagag gtcgcgcaga cgttaccgtc ggcgccgcgc gagccggccg cggcgccgac
     1381 gatcccgccg attacacccg caccgctcgt ggcggaaacg gcgccgcccg ccgcgccgat
     1441 ggcgggcgtg tccgcgcccc cgccgtccgc ctggcccccg gacgcagagt atcctgtacc
     1501 gagcatggta tccgcgccgg tgcccacgac gccgggcgtg cccgcgccgg tctacgaggc
     1561 gccggttgcc gtgccgcccg cgccgggcta tcaggcgccg gttgccgtgc cgcccgcgcc
     1621 tgcttaccag gcgccggttg cggtgccgcc tatgccgcct cgcgtcgaga ggggggcacc
     1681 cgcggcgcgg cccgccggcc ggtcaggccg cacgctggcc ttcgcggcag gtgggctgct
     1741 gatcgcgttg ctgctgctgg ccggcgggtt ggcggcactg gccggtaaca agccacagcc
     1801 gcccccggcc cccacgccga ccgtcctggc ggcgaacacc ggcgcgaccg cgactgccgc
     1861 ggcagccggc gcggcggacg ccaccggcac ggcgcagggg gcgctggcgc aaagcgctgc
     1921 caccggcacc gcggcagcgc aaggggccgc gcaggcgacg ggtacggttc tggcccaggc
     1981 gactgctgcg gcgcagagcc aggcggcggg ccaggccacg gccaccgccc agttcgaggc
     2041 cgccgcccag gccacggctg ccgcgcagac gcaagatgcc caggcccagg ccaccgccgc
     2101 cgcccaggcg caggcggccg ccgccgaag
//
LOCUS       DASUYC010000128         3032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1322780, whole genome shotgun sequence.
ACCESSION   DASUYC010000128 DASUYC010000000
VERSION     DASUYC010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322780"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..786)
                     /gene="hpt"
                     /locus_tag="VF276_02100"
     CDS             complement(184..786)
                     /gene="hpt"
                     /locus_tag="VF276_02100"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX6605683.1"
                     /translation="MRGGLAVPGARALDAAIAEVLIDEATLQARVRALGAEITARYAD
                     GPAPVLVGVIKGVTFFMADLVRAIDLPVEMDFLAISRYGPHARGSRAAVAVLYDLTTP
                     ITGRPVILVEDVIDTGLPVAFLLRMLRARQPASLEVCALLDRQSVRLIDIPLTYVGFR
                     IPDRFVVGYGLDYQEKYRNLPYIGVLRPDHAEDSLRPLAG"
     gene            complement(771..1259)
                     /locus_tag="VF276_02105"
     CDS             complement(771..1259)
                     /locus_tag="VF276_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605684.1"
                     /translation="MDQQANPAAPAPQEPAPHLPGVPVPTGPADAASPSPDPYTVAGV
                     RFAHRAEVEFAQLLEFYQIEWRYEPDEFPLEWRDGHVTRQFRPDFYLPEYDLYIELTV
                     MKQSLVRRKNRKLRLLRQLYPHIQIKLLYRRDFQRLLAGFGLPTAALEPPATGSDKCA
                     AA"
     gene            1678..2310
                     /gene="udk"
                     /locus_tag="VF276_02110"
     CDS             1678..2310
                     /gene="udk"
                     /locus_tag="VF276_02110"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEX6605685.1"
                     /translation="MGMQPLVIGIAGGSGSGKTTVTNAIIAAVGAEQVALLQQDSYYR
                     DFGALSPEQRLAINWDHPDALEDELLIAHVAALKAGQPVAVPCYDFRNYRRLAATVPV
                     APRPVIIVEGILVLVEKELRDLMDIKVFVDIDADVRFVRRLERDITERGRSVESVIAQ
                     YMSTVRPMHLEFVEPSKRYADVIVPRGGHNRVAIGMLVARVQAALHSGDG"
     gene            2313..>3032
                     /locus_tag="VF276_02115"
     CDS             2313..>3032
                     /locus_tag="VF276_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HEX6605686.1"
                     /translation="MHIWIGFLSSFLASAVEVVEAVTLVLAAGVTRGWRSTWIGVVCA
                     ALALAVLVVVFGPALEGLIPLDVLRIVIGTLLLIFGLQWLRKAIMRYTGLKELHDESR
                     IYQREVAELQAAPGAQAGMDWTAFTVAFKGVLLEGLEVVFIVITFGLSAGDVMPPLLG
                     AVAAAVVVTAVAVVIHQPLSRVQENTLKFVVGIMLSAFGTFWAGEGIGLEWPGADLAI
                     LGLLAFYAVVAGLIILALRGQA"
BASE COUNT          493 a          938 c         1099 g          502 t
ORIGIN      
        1 agtctcgtct cgtaatgctg cctaccgctt ccagtcgtcc ttgtcactct gagcgcagcg
       61 aagagtctcg gcgccgcacc ttcccgctcg aagacgagat tcttcgctgc gctcagaatg
      121 acaatactgg tggacactct attcagaagg acaacggagg ctcgaagcag tactgcggcg
      181 gatctaaccc gccaggggcc ggaggctatc ttcggcgtgg tccgggcgca gcacgccgat
      241 gtagggcagg ttgcggtact tctcctggta gtcgaggccg tagccgacca cgaagcggtc
      301 ggggatgcgg aagcccacgt aggtcagcgg gatgtcgatg aggcggacgc tttgccggtc
      361 gagcaaggca cagacttcga gcgaggccgg ttggcgggcg cgcagcatgc gcagcaggaa
      421 ggccaccggc agcccggtgt cgatcacatc ttcgaccagg atcacgggcc gcccggtgat
      481 cggcgtggtc aggtcgtaga ggacggcgac tgccgcccgg ctgccccgcg cgtgcgggcc
      541 gtagcggctg atggcgagga agtccatttc caccggcagg tcgatggcgc gcaccaggtc
      601 ggccatgaag aaggtcacgc ccttgatcac gccgaccagc accggcgccg ggccatccgc
      661 gtagcgggcg gtgatctccg cgccgagcgc gcgcactcgc gcctgcaacg tggcttcgtc
      721 gatcagcact tcggcgatgg cggcatccag cgcgcgggcg ccgggaacag ctaggccgcc
      781 gcgcacttgt cagatccggt agccgggggt tcgagggcgg cggtgggcag tccgaagccg
      841 gccagcaggc gctggaagtc gcggcgatag agcagcttga tctggatgtg cgggtaaagc
      901 tggcgcagga ggcgcagctt gcggttcttg cgccgcacca ggctctgctt catcaccgtc
      961 agttcgatgt acagatcgta ctccggcaga tagaagtcgg ggcggaactg gcgggtcacg
     1021 tgcccgtcgc gccactccag cggaaactcg tcgggttcgt agcgccactc gatctggtaa
     1081 aattcgagca gttgggcgaa ttcgacctcg gcgcggtggg cgaagcgcac accggcgacg
     1141 gtgtaggggt cgggggacgg agatgcggcg tccgcggggc cggtggggac cgggacgccc
     1201 ggaaggtgcg gggccggctc ctggggggcc ggcgcggcgg gattcgcttg ttgatccata
     1261 agtaccctgc gtgtgacgct tcgcggcgcg agcccacgtc acggggcgcc acgactctac
     1321 ataacgcaat cttattataa cacggacgcc gctgcgatac aagagccggc actactgttg
     1381 tggcaagcag atcatgtacc atagcgcaac aagcgcgcgg cccgcgccac ggctgccgga
     1441 atagcggtgt aggggccggt ttcaaaccgg cccgcgccac ggctgccgga atagcggtgt
     1501 aggggccggt ttcaaaccgg cccgtggcgg gcaccatctg taaccgggaa ccgctcaaac
     1561 agggcggcgg gacaatggcc cacttcttga tcagtcctgg gcgacccggc cacccgccat
     1621 gagcggtcgg ggcagcgggg caggatcgaa ctctggggat agcgaaggaa ctgcggcatc
     1681 ggcatgcaac cactggtcat cggcatcgcg gggggcagcg gatcgggcaa aaccaccgtg
     1741 accaacgcga tcatcgccgc ggtcggcgcc gagcaggtgg cgctgctgca acaggactcc
     1801 tattaccgcg acttcggcgc gctctcgccg gagcagcggc tggcgatcaa ctgggaccac
     1861 cccgacgcgc tggaagacga actgctgatc gcccatgtcg cggccctcaa ggcggggcag
     1921 ccggtggcgg tgccctgcta cgatttccgc aactaccggc gcctggccgc gacggtgccc
     1981 gtggcgcccc gcccggtgat tatcgtcgaa ggcatcctcg tgctggtcga aaaggaactg
     2041 cgcgacctga tggacatcaa ggtcttcgtc gatatcgacg ccgacgtgcg cttcgtgcgc
     2101 cgcctggagc gcgacatcac cgagcgcggg cggtcggtcg agtcggtgat cgcgcagtac
     2161 atgagcacgg tgcggcccat gcacctggag tttgtcgagc cgagcaagcg gtatgctgat
     2221 gtgatcgtgc cgcgcggcgg ccataaccgc gtggctatcg gcatgctggt cgccagggtc
     2281 caggccgccc tgcacagcgg agacggataa tcatgcacat ctggatcggg ttcttatcga
     2341 gctttctcgc cagcgccgtg gaggtggtcg aggcggtgac gctggtgctg gccgcggggg
     2401 tcacgcgcgg ctggcgcagc acctggatcg gcgtggtgtg cgccgcgctg gccctggcgg
     2461 tgctggtggt ggtgttcggg ccggcgctgg agggcctcat cccgctggac gtgctgcgca
     2521 tcgtcatcgg cacgctgctg ctgatcttcg ggctacagtg gctgcgcaag gccatcatgc
     2581 gttacaccgg gctgaaagaa ttgcacgacg agtcgcgcat ctaccagcgc gaggtggccg
     2641 aactgcaagc cgcgccgggc gcgcaggcgg gcatggactg gacggcgttt accgtggcct
     2701 tcaagggcgt gctgctcgaa gggctggaag tggtcttcat cgtgatcacc ttcggcctga
     2761 gcgccgggga tgtcatgccg ccgctgctcg gcgcggtggc cgccgccgtg gtcgtgaccg
     2821 ccgtggccgt tgtcatccac cagccgctga gccgggtgca ggagaacacg ctcaagttcg
     2881 tcgtcgggat catgttgtcg gccttcggca ccttctgggc cggggaaggc atcggcctgg
     2941 agtggccggg cgcggacctg gcgatcctgg gcctgctggc gttctacgcg gtggtggccg
     3001 ggctgatcat cctggccctg cgcggccagg cc
//
LOCUS       DASUYC010000129         4159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1483721, whole genome shotgun sequence.
ACCESSION   DASUYC010000129 DASUYC010000000
VERSION     DASUYC010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1483721"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VF276_02120"
     CDS             <1..496
                     /locus_tag="VF276_02120"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEX6605687.1"
                     /translation="RSMLRAELWRDSHRIHAFDALNDVVASRAALSRVVDCKLMVNGE
                     HMTTFVADGVILATPTGSTAYAMAAGGPIMHPEVRGILVTPIAPYLSIIRSLVLPEDS
                     QIELDITTDDAAFLTVDGQTHVPLEDGDAIRITTSPVPALFARLQPRPYFAATLAQRL
                     QRKD"
     gene            complement(568..3069)
                     /locus_tag="VF276_02125"
     CDS             complement(568..3069)
                     /locus_tag="VF276_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6605688.1"
                     /translation="MPAIAEIFETMEYGPAPEAPDPALAWLDARGRELKLFINGEWVA
                     PQSGQYIETISPATNKPLTRVAAAGPADVDAAVAAARAAFPGWSGLSGHARARYLYAL
                     ARHIQKHSRLLAVLEALDNGKPIRETRDLDIPLVARHFYYHAGWAQLMETELRDYRPL
                     GVVGQIIPWNFPLLMLAWKIAPALAMGNTVVLKPAKFTPLSALAFAQIVEEVGLPPGV
                     VNILTGEAGQVGEALVSHPDVDKIAFTGSTDVGRHIRQVTAGSGKRLSLELGGKSPFV
                     VFDDADLDSVVEGVVDAIWFNQGQVCCAGSRLLVQENVAERLYARLRARMEKLRVGDS
                     LDKAVDIGAIAGPVQLETVRRFVDQGVAEGATLWQPSWACPTEGCYYPPTLFTNVAPA
                     ANIAQAEIFGPVLVAMTFRSPSEAVALANNTRYGLAASVWSDNINLALDVARRIKAGT
                     VWVNCTNLFDAASGFGGYRESGFGREGGKEGLYEYIRPAWEAEALQAASSEPAEDGTQ
                     AAQGSNGKRKHTRAAHGPAQTVRLRANGHGNGHHDGLPSLGNLPGIDRTPKMYIGGKQ
                     ARPDSGYSRPVYDATGQQIGEVGEGNRKDIRNAVEAAHKASGWGNSTGHLRAQILYYI
                     AENLAARADEFATRITESTGRSAEDAESEVAASIDRLFTYAAWADKFDGQVHHTPLRN
                     VTIAMPEAIGVMGIACSTEMPLLGFIALVAPAIAMGNTVVAIPSEAHPLSATDLYQVF
                     DTSDLPAGVVNIVTGARDTLTRVLAEHDDVDAVWYFGSAEGSTMVETAGAGNMKRTWV
                     NYGHLRDWRDPVQGAGEDFLREATQVKNIWVPYGE"
     gene            complement(3091..3441)
                     /locus_tag="VF276_02130"
     CDS             complement(3091..3441)
                     /locus_tag="VF276_02130"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HEX6605689.1"
                     /translation="MLRLAADENFNNIILRGARRRNPGIDIVRVQDVGPVGADDPTVL
                     EWAAQEGRILLTHDSSTLIGFAYARVASGQPMPGVLAVKRTVPITIAIEDILLIAECG
                     EANEWEGLVRYLPL"
     gene            complement(3445..3780)
                     /locus_tag="VF276_02135"
     CDS             complement(3445..3780)
                     /locus_tag="VF276_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016951837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEX6605690.1"
                     /translation="MSLTIETEPVPLAINQQGVVRVGGTRVTLDTVVAAFEEGATAEE
                     IVLQYPVLKLSDVYLVIGYYLRHRAEVEAYLQQQQELADRIYEENKKRLDPDGLRDRL
                     LARRAERKV"
     gene            complement(3822..4133)
                     /locus_tag="VF276_02140"
     CDS             complement(3822..4133)
                     /locus_tag="VF276_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX6605691.1"
                     /translation="MAAKNTSTDINELLHAKREDILRTAARHGASNVRVFGSVARGEA
                     GPSSDIDFVVELAPDRSLLDQVALLQDLEDLLGRRVDVVEPEGLHWYIRDRVLKEAVP
                     L"
BASE COUNT          652 a         1389 c         1350 g          768 t
ORIGIN      
        1 ccgctcgatg ttgcgggccg aattgtggcg cgacagccac cgcatccacg ccttcgacgc
       61 gctcaacgac gtggtggcaa gccgtgccgc cctctcgcgg gtggtcgact gcaagctgat
      121 ggtcaacggg gagcacatga ccaccttcgt cgccgacggg gtgatcctgg cgacgccgac
      181 cgggtccacg gcgtatgcca tggctgcggg cgggccgatc atgcacccgg aggtgcgcgg
      241 catcctagtc acgcccatcg cgccctacct gagcatcatt cgtagcctgg ttttgccgga
      301 agacagccag atcgagctcg acatcaccac cgacgacgcg gctttcctca cggtggacgg
      361 tcagactcac gtgcccttgg aagacggcga cgccattcgc atcacgacca gcccggtccc
      421 ggcgctcttt gcccggctcc aaccgcgccc ctatttcgcc gccacactgg cccagcgcct
      481 gcaacgcaag gactgatcgc gcccgccagg gcaaaaacga ggccgccgag cggaatgccc
      541 ggcggcctgc ttgcggtccg gctggatcta ctctccgtag ggcacccaga tgttcttgac
      601 ctgggtggcc tcgcgcagga agtcttcgcc cgcgccctgc accgggtcgc gccagtcgcg
      661 caggtggccg tagttgaccc aggtgcgctt catgttgccc gcgccggccg tctcgaccat
      721 cgtgctgccc tcggcgctgc cgaagtacca gacggcgtcc acgtcgtcgt gctcggccag
      781 cacgcgggtc agcgtgtcgc gcgcgccggt gacgatgttg accacgcccg cgggcaggtc
      841 ggaggtgtcg aacacctggt agaggtcggt ggcgctgagc gggtgcgcct ccgaggggat
      901 cgccacaacg gtgttgccca tggcgatggc cggggcgacc agggcgatga agccgagcag
      961 cggcatctcg gtggagcagg cgatgcccat gacgccgatg gcttcaggca tggcgatggt
     1021 cacgttgcgc agcggcgtgt gatgcacctg gccgtcgaac ttgtcggccc aggccgcgta
     1081 ggtgaagagg cggtcgatgc tggccgcgac ctcgctctcg gcgtcctctg ctgagcggcc
     1141 cgtggattcg gtgatgcgcg tggcgaactc gtcggcgcgg gcggccaggt tttcggcgat
     1201 gtagtagagg atctgcgcgc gcaggtggcc ggtgctgttg ccccagccag acgccttgtg
     1261 cgccgcttcg acggcgttgc ggatgtcctt gcggttaccc tcgcccacct cgccgatctg
     1321 ctgcccggtg gcgtcgtaga ccgggcggct gtagccggag tcgggccgcg cctgcttgcc
     1381 gccgatgtac atcttgggcg tgcggtcgat gcccggcaga ttgcccaggc tgggcagacc
     1441 gtcgtggtgg ccgttgccgt ggccgttggc gcgcaggcgc acggtttgcg ccgggccgtg
     1501 cgcggcgcgg gtgtgtttgc gcttgccgtt gctgccctgg gcggcttgcg tgccgtcctc
     1561 cgccggttcg ctcgacgcgg cctgcaaggc ttcggcttcc caggccgggc ggatgtactc
     1621 gtacaggccc tctttgccgc cctcgcgccc gaagccgctc tcgcggtagc cgccgaagcc
     1681 cgacgccgcg tcgaacagat tggtgcagtt gacccagacc gtgcccgcct tgatgcgccg
     1741 ggccacgtcg agcgccaggt tgatgttgtc gctccagacg ctggcggcca ggccgtagcg
     1801 cgtgttgttc gccagggcca cggcttcgct gggcgagcgg aaggtcatgg cgaccagcac
     1861 cgggccgaag atctcggcct gggcgatgtt ggccgccggg gccacgttgg tgaacagggt
     1921 gggcgggtaa tagcagccct cggtcgggca ggcccacgac ggctgccaga gcgtcgcgcc
     1981 ctcggccacg ccctggtcca cgaaccggcg caccgtctcc aactgcaccg gcccggcgat
     2041 ggccccgatg tccacggcct tgtccagcga gtcgccgacg cgcagctttt ccatgcgcgc
     2101 gcgcagccgg gcgtagaggc gttcggcgac attctcctgc accagcaggc gcgaccccgc
     2161 gcagcagacc tggccctggt tgaaccagat ggcgtcgacc acgccctcga ccacactgtc
     2221 caggtcggcg tcgtcgaaga ccacgaacgg cgacttgccg cccaactcca gcgacaggcg
     2281 cttgccgctg cccgcggtga cctggcggat gtggcggccg acgtcggtcg agccggtgaa
     2341 ggcgatcttg tccacgtcgg ggtggctgac cagcgcctcg cccacctggc ccgcctcgcc
     2401 ggtcaggatg ttgaccacgc ccggcggcaa ccccacctct tccacgatct gggcgaaggc
     2461 cagcgcactc agcggggtga acttggcggg cttgagcacc accgtgttgc ccatcgccag
     2521 cgccggggcg atcttccagg ccagcatgag caaggggaag ttccagggga ttatctggcc
     2581 gacgacgccg agcggccggt agtcgcgcaa ctcggtttcc atgagttgcg cccagcccgc
     2641 gtggtagtag aagtggcggg ccaccagcgg gatgtcgagg tcgcgcgtct cgcggatcgg
     2701 cttgccattg tcgagcgctt cgagcacggc cagcaggcgt gagtgcttct ggatgtgccg
     2761 ggcgagggcg tacagatagc gggcgcgggc gtggcccgac aggccgctcc agccggggaa
     2821 ggcggcgcgc gccgccgcca cggcggcatc cacgtcggcg ggcccggcgg cggccacgcg
     2881 ggtcagcggc ttattggtgg ccgggctgat cgtctcgata tactgcccgc tctgcggcgc
     2941 cacccactcg ccgttgatga acagcttcag ttcgcgcccg cgtgcgtcga gccaggccag
     3001 cgccgggtcg ggagcctcgg gcgccgggcc atactccatc gtctcaaaaa tctccgcgat
     3061 tgcaggcatg gatgcctcct cgttttgtac tcataagggc aggtaacgta ctagcccttc
     3121 ccactcattt gcttcgccac attccgctat cagcaagatg tcttcaatag caatggtaat
     3181 tggaacagta cgctttaccg ctaacacacc tggcattggc tgtccagacg caactcgtgc
     3241 gtaggcaaac cctataagcg tcgatgagtc atgtgtcagc aatatacgtc cttcttgcgc
     3301 cgcccactct agcactgtcg gatcgtcagc cccaacagga cccacatctt gaactctgac
     3361 gatatcaatt ccaggattac gtctgcgcgc accccgaagg atgatattat taaagttttc
     3421 gtcggctgct agcctgagca ttgctcagac cttgcgttcg gcacggcgag ccagcagacg
     3481 atcccgtaac ccgtcgggat caagacgttt cttattctct tcgtatatgc gatctgccag
     3541 ttcttgctgc tgttgtaaat aagcttcgac ttcagctcgg tgccgtagat aatagccaat
     3601 taccaaatag acatcggaca gcttgagcac gggatattgt agaacgattt cctccgccgt
     3661 tgcgccttcc tcaaacgcgg cgacgaccgt gtcgagcgtg acgcgtgttc cgcccacccg
     3721 cacgactccc tgctggttga ttgccaatgg cacaggttct gtttcaatag tgaggctcat
     3781 agtttgatct cccacgtagt gcagttagct tataaacgcg ctcacagcgg cacggcctct
     3841 ttgagtaccc ggtcgcggat gtaccagtgc aagccttcgg gttctaccac gtccacccga
     3901 cgcccgagca gatcctccag gtcctgaagc agggccactt gatcgagcag gctacgatcc
     3961 ggcgccagtt ccaccacgaa gtcgatgtcg ctgcttggcc cggcttcgcc gcgggcaaca
     4021 gagccaaaca cgcgcacatt agacgccccg tgccgtgccg ccgtgcgcaa gatatcctcg
     4081 cgcttcgcgt gcagcagttc gttgatgtct gtgctcgtat tcttggcggc catattcatg
     4141 cactccatcc cgctgggcg
//
LOCUS       DASUYC010000130         2509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_2018100, whole genome shotgun sequence.
ACCESSION   DASUYC010000130 DASUYC010000000
VERSION     DASUYC010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2018100"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="VF276_02145"
     CDS             <1..862
                     /locus_tag="VF276_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX6605692.1"
                     /translation="NDDSRRIAGHLLEFLTHEVRKGRLPANLLPLQSGVGNIANAVLM
                     GLQESPFEQLTSYTEVIQDGMLALMRSGKLLMASATAFALSAGGVAELTAHLDEYRQR
                     IILRPQEISNNPEVIRRLGCLSMNGLIEADIYGNVNSTHVMGTRIQNGIGGSGDFTRN
                     AYISCFMTPSQAKNGAISCIVPMVAHVDHTEHDVQVIVTEQGLADLRGLAPKQRTRQI
                     IENCAHPDFRPALRDYYRRALDGAPGKQTPHLLEEAFSWHLRYQATGTMRAQPDAPPA
                     EPVPADAARP"
     gene            859..>2509
                     /locus_tag="VF276_02150"
     CDS             859..>2509
                     /locus_tag="VF276_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase"
                     /protein_id="HEX6605693.1"
                     /translation="MSRRTRAGRAVPVARDGAPWPLGVTWVPEARAYNFSLYAGHAYC
                     VTLLLYTADDPVSPVLQYRMNYLQNKSHYIWHCRLPASAVRTARYYAYQVEGPTVLEA
                     GLRFDPQKILLDPYAPAVFFPPLFSRAAARRPGCNAGRAPLGVIPHGPERFNWNHDRR
                     PRHTSDTIIYEMHVRGFTERANSGVAPDRRGTFAGVIDKIPYLQELGVTAVELLPVFQ
                     FDPQEGNYWGYMPLNFFALHQTYAGAQDPDAARQEFRAMVQALHAAGIEVILDVVYNH
                     TTEEDAAGPTYSFRGIDNPTYYLLEGERQHYCNDAGTGNVLRCANPYVRKLILDSVRF
                     WAREMHVDGFRFDLASILTRNSNGSINLADPPVIIEISSDPDLADLRFIAEAWDISSY
                     QLGRSFPGTTWLQWNGEFRDDVRAFVRGDPGKVGDLMARLYGSPDLFPDTLADAYHPY
                     QSVNYVTAHDGFCLYDLVAYNRKHNEANGHHNTDGPSENLSWNCGWEGDAGAPGEVTA
                     LRKQQVKNFCCLLMLANGTPMFSAGDEFLQTQHGNNNPYNQD"
BASE COUNT          453 a          888 c          762 g          406 t
ORIGIN      
        1 gaacgacgac tcccggcgca tcgcgggcca cctgctggag ttcctgaccc acgaagtgcg
       61 caagggccgg ctgccggcga acctgttgcc cctgcagtcg ggcgtcggca atatcgccaa
      121 cgcggtgctc atgggcctgc aggaaagccc gttcgagcag ctcacctcct ataccgaggt
      181 catccaggac ggcatgctgg cgttgatgcg ctccggtaag ctgctgatgg cctcagcgac
      241 agccttcgcc ctgagcgccg gcggcgtggc ggagttaacc gcacacctgg acgaataccg
      301 gcagcgcatc atcctgcgcc cccaggagat cagcaacaac ccggaagtca tccgccggct
      361 gggctgcctg tcgatgaacg ggctgatcga ggccgacatc tacgggaacg tgaactcgac
      421 ccacgtcatg ggcacgcgca tccagaacgg catcggcggc tcgggcgatt tcacccgcaa
      481 cgcctacatc tcctgcttca tgactccctc gcaggcgaag aacggcgcca tctcctgcat
      541 cgtgccgatg gtggcccatg tggaccacac ggaacatgac gtgcaggtga tcgtgaccga
      601 gcagggcctg gccgatctgc gcggtctcgc gcccaaacaa cgcacccggc agatcatcga
      661 gaactgcgcg cacccggact tccgtcccgc gctgcgcgac tactaccggc gcgcgctcga
      721 cggggcgccc ggcaagcaaa caccgcacct gctggaggag gctttctcct ggcacctgcg
      781 ctaccaggcc accggcacca tgcgcgccca gcccgacgcg ccccctgccg aaccggtgcc
      841 cgccgacgcg gcacggccgt gagccgccgg acgcgcgcag ggcgcgccgt cccggtcgcc
      901 agggacggtg cgccctggcc gctgggggtc acctgggtgc ccgaggccag agcgtataac
      961 ttctcgctgt acgcgggcca cgcttactgc gtgaccctgc tgctgtacac ggcggacgac
     1021 ccggtgtctc cggttctgca gtaccggatg aactatctgc agaacaagtc acactacatc
     1081 tggcactgcc ggctgccggc ctccgccgtc cgcaccgctc gctactacgc ctaccaggtg
     1141 gaagggccga ccgtgctcga ggcgggcttg cgctttgacc cgcagaagat cctgctggat
     1201 ccctacgcgc ccgccgtctt cttcccacct cttttcagcc gcgccgccgc ccgccgcccc
     1261 ggatgcaacg cgggccgggc gcccctcggc gtcatccctc acggcccgga acgcttcaac
     1321 tggaatcacg atcggcgtcc gcgccacacc tccgacacga tcatctatga gatgcacgtg
     1381 cgcggtttta cggagcgcgc caactccggc gtcgcgcccg atcgccgggg cacctttgcc
     1441 ggcgtgatcg ataagattcc ctatctacag gaattggggg tgacggcggt ggagttgctg
     1501 cccgtcttcc agttcgaccc gcaggagggc aattactggg gctatatgcc gctcaacttc
     1561 ttcgccctgc accagaccta cgccggcgcg caagacccgg atgccgcccg gcaggaattc
     1621 cgcgcgatgg tgcaggccct gcacgcggcg gggatcgagg tcatcctgga tgtggtctat
     1681 aaccatacga cggaagaaga tgccgccggg ccgacctata gcttccgcgg catcgacaac
     1741 cccacctact acctgctgga aggcgagcgg cagcactact gcaacgacgc gggcaccggc
     1801 aacgtcttgc gctgcgccaa cccctatgtc cgcaagctca tcctggacag cgtgcgcttc
     1861 tgggccaggg agatgcacgt cgacgggttt cgcttcgacc tggcgtcgat cctgacgcgc
     1921 aacagcaacg gatcgattaa cctggccgat ccgcccgtca tcatcgagat cagttccgac
     1981 ccggacctgg cggacctccg ctttatcgcc gaagcctggg atatttccag ctatcagttg
     2041 gggcgcagct ttccgggcac tacctggctg caatggaacg gcgagttccg cgacgatgtg
     2101 cgcgccttcg tccgcggcga ccccggcaaa gtcggcgatc tcatggcccg gctgtacggc
     2161 agtcccgatc tattcccgga tacgctggcc gatgcctacc atccctacca gagcgtgaac
     2221 tatgtcaccg cccacgacgg cttctgcttg tacgatctgg tggcctacaa tcgcaagcat
     2281 aacgaggcga acgggcatca caacacggac gggcccagcg agaacctgag ctggaactgt
     2341 ggctgggaag gcgatgccgg cgcgcccggc gaggtgacgg ccctgcgcaa gcagcaggtc
     2401 aagaacttct gctgcctgct gatgctcgcc aacggcacgc cgatgttcag cgcgggcgac
     2461 gagttcctgc agacccagca tggcaacaac aacccctaca accaggaca
//
LOCUS       DASUYC010000131         2193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_759497,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000131 DASUYC010000000
VERSION     DASUYC010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759497"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1773
                     /locus_tag="VF276_02155"
     CDS             <1..1773
                     /locus_tag="VF276_02155"
                     /inference="COORDINATES: protein motif:HMM:NF019758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605694.1"
                     /translation="EDTVTEWDQAVAAVPEGCLIGLPAGEELLSEYEALGRAAQTGRE
                     RLRKLSTARERLAAQRTALPWTRPDRETLRRLLRVFPPGGVVHIENRGWAVYLGPRPG
                     GAHLFWLGDHVIALTGYSAIDYVPPQRPQVTLPPELADLAAGDIAAAPGERIPVSHEA
                     RAWMAEQLAGIDLPDLEQWILSHQQTQGAEIEEQLQQFDTQVAEAEEYVHRVAAEERA
                     HACHTCPVRKQHRHYRRERGRLLAGRQEAVDYLEGRRAYEDTRLQALLDGLVAVLRRF
                     YYLTRDGEKTAKAARLMDIFDTNALMISELIEGGYLETLTGPDVAEVFSWFAYDRDIE
                     FRNRNLLPGHLVQLRRQMDELQRDIFGAERRHDLMITTGYNPYFYGVVRSWCRGASMA
                     GLLAKVELSEGDIVMAMTKTLDLMRQVREMLLRHNPDNPLYAALREAEGLLRRGVVEM
                     VNSIGFVRSGPESPAPGAVAPETPAPAKPAGSGFADVDRMLGILADPNSTPPPIHPPD
                     TELQRPDRPARFPRPERDGEPARSPRRTGYPGTGRDRGARPPASGGRERDARSPYSGG
                     THPKHRTRPGRDTRPGHRPRRGGG"
     gene            1776..>2193
                     /locus_tag="VF276_02160"
     CDS             1776..>2193
                     /locus_tag="VF276_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605695.1"
                     /translation="MDIWAALATLDALGLPRDSYAVFGSGPLGARGLRESGDLDIIVA
                     PALWAELAQRYPIRHKAEGAAVVIGDIEVWDRWHPAAGPLADLIAGAEEIRGYRFVRL
                     DKVLQWKAAGNRPKDRADAELIRRYLAEHRGPPGGGS"
BASE COUNT          330 a          816 c          770 g          277 t
ORIGIN      
        1 gaagacacgg tgaccgagtg ggaccaggcg gtggccgccg tgcccgaagg ctgcttgatc
       61 ggtctgcccg cgggcgagga actgctcagc gagtacgagg cgctgggccg ggccgcgcaa
      121 acgggccgtg agcgcctgcg caagctgagt acggcccgcg aacggctggc cgcccagcgc
      181 acggccctgc cctggacgcg ccccgaccgg gaaacgctgc gccggctgtt gcgcgtgttc
      241 ccgcccggcg gggtggtcca tatcgagaac cgcggctggg ctgtctatct ggggccgcgc
      301 cccggtggtg cccacctctt ctggctcggc gaccacgtga tcgcgctcac cggctacagc
      361 gccatcgact atgtgccgcc gcagcgcccg caggtgacat tgccgcccga actggccgac
      421 ctggccgcag gcgacatcgc cgcggcgccc ggcgagcgca tcccggtcag ccacgaggcg
      481 cgcgcctgga tggccgagca actggccggc atcgacctgc cggacctgga gcaatggatc
      541 ctgagccatc agcagacgca gggcgcggag atcgaggagc aactccagca gttcgacacg
      601 caggtggccg aggccgagga gtatgtgcac cgggtggccg ccgaggagcg ggcccacgcc
      661 tgccacacct gcccggtgcg caagcagcac cgccactacc ggcgcgagcg cgggcgcctg
      721 ctggccgggc ggcaggaggc cgtggactac ctggagggcc gccgggccta cgaggacacg
      781 cgcctccagg cgctgctgga cggcctggtc gcggtgctgc gccgcttcta ctacctgacc
      841 cgcgacggcg agaagaccgc caaggcagcg cgcctgatgg acatcttcga caccaacgcg
      901 ctcatgatct cggagttgat cgagggcggc tacctggaaa cgctgaccgg ccccgatgtg
      961 gccgaggtct tctcctggtt cgcctatgac cgcgacatcg agtttcgcaa ccgcaacctg
     1021 ctgcccggcc acctggtcca actgcgccgc cagatggacg aattgcagcg cgacatcttc
     1081 ggggccgagc ggcgccacga cctgatgatc accaccggct acaacccgta cttctacggc
     1141 gtggtccgct cgtggtgccg gggcgccagc atggccggtc tgctcgccaa ggtggagctg
     1201 agcgagggcg acatcgtcat ggcgatgacc aagacgctcg acctgatgcg ccaggtgcgc
     1261 gagatgctgc tgcgccacaa ccccgacaac ccgctctacg ccgccctgcg cgaagccgag
     1321 ggcctgctgc gccggggcgt ggtcgagatg gtgaacagca tcggcttcgt gcgcagtggc
     1381 cccgagtcgc ccgcgccggg cgcggtagcc ccggagacac ccgcccccgc gaagcccgcc
     1441 ggcagcggct tcgccgacgt ggatcgcatg ctgggcatcc tggccgaccc caactccacc
     1501 ccgccgccca tccacccgcc ggataccgag ttacagcgcc ccgaccgccc ggcccgcttc
     1561 ccccgtcctg agcgcgatgg ggaaccggcg cggtcgccgc gccgcaccgg ctaccccggc
     1621 acgggccgcg accggggcgc gcgcccgccg gcatccgggg gccgggaacg tgacgcccgc
     1681 tcgccctatt ccggcggcac tcaccccaag caccgcaccc gccctggccg ggatacccgc
     1741 cccggccacc gcccccggcg cggcggcggc tagcgatgga catctgggcc gccctggcga
     1801 cgctcgacgc gctcggcctg ccccgcgaca gctatgccgt gttcggtagc gggccgctgg
     1861 gcgcgcgggg cctgcgcgaa agcggcgacc tggacattat cgtggcgccc gcgttgtggg
     1921 cggaactggc ccaacgctac ccgatccggc acaaagccga gggcgccgcc gtggtgatcg
     1981 gcgacatcga ggtctgggat cgctggcacc cggccgccgg cccgctcgcc gatctcatcg
     2041 ccggggccga ggagatccgc ggctatcgct tcgtgcgcct ggacaaggtg ctgcaatgga
     2101 aagcagccgg gaaccgcccc aaggaccggg ccgacgcaga gttgattcgg cgctacctgg
     2161 ccgagcatcg gggtccaccc ggcggaggtt cgg
//
LOCUS       DASUYC010000132         2040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_759456,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000132 DASUYC010000000
VERSION     DASUYC010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759456"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VF276_02165"
     CDS             complement(<1..252)
                     /locus_tag="VF276_02165"
                     /inference="COORDINATES: protein motif:HMM:NF037041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605696.1"
                     /translation="MDQQTGSPMDTNQAGSFAGPNSKALEALLPDYLRGQRWFSAKAR
                     TITRVRLAGQVPFAPDPAGPPQAYLTFIEVQYAEGAPQTF"
     gene            complement(288..>2040)
                     /locus_tag="VF276_02170"
     CDS             complement(288..>2040)
                     /locus_tag="VF276_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="maltotransferase domain-containing protein"
                     /protein_id="HEX6605697.1"
                     /translation="FMVAEMGHYQYTVQGWLDHFKHWSRDLGKRIAAGQDVSIDLLIG
                     AQHVEEALPRATGADHDKLRGYLDTLRAGGDAATGAAFSAELADLMYRYTDRSTATTY
                     EPTLGIVVDRVRSRFSTWYEFFPRSCWTKNCTQTTLREAVAQLAYISSMGFDIVYLPP
                     VHPIGTTKRKGKNNNVVAAPGDVGSPWAIGNEAGGHKAINPELGTLDDFRSFVAKARE
                     YNMEVAMDIAFQVSPDHPYVREHPEWFKWRPDNTVQYAENPPKKYEDIYPFNFESENW
                     RELWDELKSVFDFWIGFGVKVFRVDNPHTKSLRFWEWLIAGIKQDHPDVIFLAEAFTR
                     PKVMYYLAKIGFTQSYTYFAWRNTRWELTEYFTELTKTGVAQFFRPNVWPNTPDILTE
                     HLQQGGRPAFMSRIVLAATLAASYGIYGPAYELGVNQPIAHGKEEYLNSEKYEVKHWD
                     VDNPLSLRLLIARLNQIRCDNPALQSNEGLRFVGTTNDQLIAYYKATPNMDNIILTVV
                     NLDPRNTQSGMIDVPVEELGIDPHRSYEVLDLLADNRFLWSGWQNYVELNPQVLPAHV
                     FSLRRRMNTEQNFDPFV"
BASE COUNT          352 a          595 c          681 g          412 t
ORIGIN      
        1 gaaggtctgc ggggcgccct cggcatactg gacctcgatg aaggtcaggt acgcctgcgg
       61 cggcccggcg gggtccggcg cgaaagggac ctgaccggcc agacgcacgc gcgtgatggt
      121 gcgcgccttg gcgctgaacc agcgctgccc gcgcaggtag tcgggcaaga gcgcttccag
      181 cgccttgctg ttcgggccgg cgaagctgcc ggcctggttt gtgtccatgg gcgacccggt
      241 ttgctggtcc attcgttccc cacccctccc attcatcaca caaggcgcta cacaaacgga
      301 tcgaagttct gctcggtgtt catgcgccgc cgcaggctga agacatgcgc gggcagcacc
      361 tgggggttga gttccacgta gttttgccag cccgaccaga gaaagcgatt atcggcgagc
      421 aggtccaata cctcgtacga gcggtgcgga tcgatgccca gttcttcgac cggcacatcg
      481 atcatgcccg attgcgtatt gcgcgggtcc aggttgacca cagtcaggat gatgttatcc
      541 atattcggcg tggctttata gtaggcgata agctggtcgt tggtcgtgcc gacaaagcgc
      601 aggccctcgt tgctttgcag cgccggattg tcgcagcgga tctggttgag ccgggcgatc
      661 agcaggcgca ggctcagcgg attgtccacg tcccagtgct tgacctcgta cttctcggaa
      721 ttgaggtatt cctctttgcc gtgcgcgatg ggctggttga cgcccagttc gtaggccggg
      781 ccgtagatgc cgtagctggc ggcgagcgtg gcggccagca cgatccgcga cataaacgcg
      841 ggccgcccgc cctgttgcag atgctcggtc aggatgtcgg gcgtgttggg ccagacgttg
      901 ggacggaaga actgggccac cccggtcttg gtcagttcgg tgaaatactc ggtcagttcc
      961 cagcgggtgt tgcgccaggc aaagtaggta tacgactggg tgaagccgat tttcgccagg
     1021 taatacatga ccttgggccg ggtgaaggcc tcggccagga agatcacgtc gggatgatcc
     1081 tgcttgatgc cggcaatcag ccattcccag aagcgcagcg acttggtgtg cggattgtcc
     1141 acacggaaca cttttacgcc gaagccgatc cagaagtcga acacgctctt gagttcgtcc
     1201 caaagttcgc gccagttctc ggactcgaag ttgaagggat agatatcctc atacttcttc
     1261 ggcgggttct cggcatactg gaccgtgttg tcggggcgcc acttgaacca ctccgggtgc
     1321 tcgcgcacgt agggatggtc gggcgagacc tggaaggcga tgtccatggc gacttccatg
     1381 ttgtattcgc gggctttcgc cacaaagctg cggaaatcgt ccagggtgcc cagttcggga
     1441 ttgatcgcct tgtggccccc cgcctcgttg ccgatggccc aggggctgcc cacgtcgccg
     1501 ggtgcggcca ccacgttgtt gttcttgccc ttgcgcttgg tggtgccgat cgggtggacc
     1561 ggcgggaggt agacgatgtc gaagcccatc gaggagatat aggccagttg cgctacagcc
     1621 tctctcaaag tggtctgggt gcagttcttg gtccagcaag agcgggggaa aaactcgtac
     1681 caggtgctga accgggagcg gacccgatcc accacgatgc ctaaggtggg ttcatatgtg
     1741 gtggccgtgc tgcggtcggt atagcgatac atcaggtcgg ccagttccgc cgagaaggcg
     1801 gcacccgtgg ccgcgtcacc cccggcgcgc agggtgtcca ggtagccgcg caacttgtcg
     1861 tggtcggcgc cggtggcgcg cggcagggcc tcctctacat gctgggcgcc gatgagcagg
     1921 tcaatcgaca catcctgccc ggcggcgatg cgcttgccca ggtcgcgcga ccagtgcttg
     1981 aagtggtcga gccagccctg cacggtgtac tggtagtggc ccatctcggc aaccatgaac
//
LOCUS       DASUYC010000133         2049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_989295,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000133 DASUYC010000000
VERSION     DASUYC010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989295"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1687
                     /locus_tag="VF276_02175"
     CDS             <1..1687
                     /locus_tag="VF276_02175"
                     /inference="COORDINATES: protein motif:HMM:NF019311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HEX6605698.1"
                     /translation="LGGDDDAAPIHPPAWMTYPLAVQWTGGLVVPAYGAYTFQLKAPL
                     GATLEIDGRPVLTSTGGTAPPAPGQIVLAKGVHQVRLAGTLADPRGAVELRWTTDNGV
                     AVPIDRAFLWNDPPGALLGTGYAAVSGPDWFTGANPATTAPLVVRRDGFLGWRAVNGS
                     LGGGTNVAAIWRGTLRVAQPGSYRLDPRTAGRIAVWIDGQVVGTGNVPGVTDSLPAMV
                     PLTAGDHALEVRFQATGDNALFEFYWQPPGGPRELLPPTALAPLAGGAWPAAERPDAP
                     SPNAALIGAAQPTLAVQKAGSISGNGVWMEARGVGVLPDGRVVVGDTGHAQILVYDQS
                     GKQVAAWGRPGDGDGQFATLSDIAVSGDGIIAALDAGHRDVQLFDGNGTLIAHLRNDQ
                     IGISPASGIAWGPDGKLYIADTGGGRVLRVSRGGQPEFSMSAGDATHQALSQPVDVAV
                     TADGIVYAADLIGRVVRFNAAGVIDQEWKVPVGGARGGGHLAVWGTRLAVTDPSGNSV
                     NFIDLRAANDAVLATPASAALGLAVPVGVAAGPDGRLYVLDSDNARVVILTAP"
     gene            complement(1803..>2049)
                     /locus_tag="VF276_02180"
     CDS             complement(1803..>2049)
                     /locus_tag="VF276_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605699.1"
                     /translation="PAFDLCLYNAGMDPYGECLNGGLAGINTAILAERERLVFAWCRE
                     QRIPVAFVLAGGYVGGRLTAASLVALHRLTLAAAADR"
BASE COUNT          287 a          722 c          747 g          293 t
ORIGIN      
        1 gctaggcggg gacgatgatg ccgcgccgat ccacccgccc gcctggatga cctatccgct
       61 ggccgtgcaa tggacaggcg gcctggtggt cccggcctac ggcgcctaca cctttcagtt
      121 gaaagccccg ctgggcgcca cgctggagat cgacgggcgc cccgtgttga cgagtaccgg
      181 ggggacggcg ccgcccgcgc cgggacagat tgtgctggcg aagggtgtcc accaggtgcg
      241 tctcgcaggc acgctggccg atccacgcgg cgcggtggaa ctgcgctgga ccaccgacaa
      301 cggcgtcgcg gtgcccatcg accgggcgtt tctctggaac gatccgccgg gcgccttgct
      361 gggcaccggc tacgccgccg tgtccgggcc ggactggttt accggtgcga acccggccac
      421 gacggcgccg ctcgtcgtgc gccgggacgg cttcctcggc tggcgggctg tgaacggcag
      481 cctcggcggc gggacgaatg tggccgcgat ctggcgcggc accctgcgcg tcgcccagcc
      541 gggatcttac cgcctggatc cgcggaccgc gggccgcatc gccgtgtgga tcgacggcca
      601 ggtggtgggc acgggcaatg tgccgggcgt gaccgacagc ctgcccgcca tggtgccgct
      661 cacggcgggc gatcacgcgc tggaggtgcg gttccaggct accggcgaca atgcgctctt
      721 tgagttctac tggcagccgc ccggcggccc gcgcgaactg ctgccgccca ccgccctggc
      781 gccgcttgcc ggaggcgcct ggcccgccgc cgagcgcccg gatgcgccca gccccaatgc
      841 cgcgctcatc ggcgcggccc agcccacgct cgccgtccag aaggcgggca gcatcagcgg
      901 caacggcgtc tggatggagg cccgcggcgt gggtgtgctg cccgacggcc gggtcgtggt
      961 cggcgatacc gggcacgcgc agatcctcgt ctacgaccag agcggcaagc aggtggcggc
     1021 gtggggccgc cccggcgacg gcgacgggca gttcgctacc ttgagcgaca tcgcggtgag
     1081 cggcgacggc atcatcgccg cgctcgacgc cgggcaccgc gatgtgcaac tcttcgacgg
     1141 caacggcacg ctcatcgccc atctgcgcaa cgaccagatc ggtatctccc cggccagcgg
     1201 catcgcctgg ggtcccgacg gcaagctcta tatcgccgac acgggcgggg ggcgcgtgct
     1261 gcgcgtgagc cgcggcggcc agccggagtt cagcatgagc gccggcgatg ccacgcacca
     1321 ggcattgagc cagccggtgg acgtggccgt caccgcggac ggcatcgtct atgccgccga
     1381 cctgatcggg cgagtggtgc gcttcaacgc ggcgggggtg atcgaccagg agtggaaggt
     1441 gccggtcggc ggggccagag gcggcggcca cctggccgtc tggggcaccc gcctggccgt
     1501 gaccgatccc tcgggtaaca gcgtgaactt tatcgatctg cgtgcggcga acgacgccgt
     1561 gctggctacc cccgcttccg ccgcgctggg cctcgccgtg ccggtgggcg tggccgccgg
     1621 ccccgatggg cgcctctatg tgctcgacag cgataacgcg cgcgtcgtga tcctcaccgc
     1681 gccttaacgg cccggccccc atctattgtc actctgagcg cagcgaagag tctcgtcgtt
     1741 tgggctacca gacggagatt cttcgctacg ctcagaatga cgggggtggt gtgacgcgcg
     1801 ccttagcggt ccgctgcggc ggccagggtc agccggtgga gcgcgaccag gctcgccgcg
     1861 gtgagccgtc ccccgacata gccgcccgcc agcacgaagg cgacggggat gcgctgttcg
     1921 cggcaccagg caaagaccag gcgctcgcgc tcggccagga tggccgtgtt gatgcccgcc
     1981 aacccgccgt tcagacactc gccatatggg tccatgcccg cgttatacag gcagaggtca
     2041 aaggcaggg
//
LOCUS       DASUYC010000134         2256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1784791, whole genome shotgun sequence.
ACCESSION   DASUYC010000134 DASUYC010000000
VERSION     DASUYC010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1784791"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VF276_02185"
     CDS             <1..264
                     /locus_tag="VF276_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605700.1"
                     /translation="VIGGLVYLLADREQEGAAPLAEPAPPQEVGDAVAQRRKEQAAVA
                     AVAVALALTREAEAVHPAVRGAPITPWQNIMRGRQALERGRRR"
     gene            261..707
                     /locus_tag="VF276_02190"
     CDS             261..707
                     /locus_tag="VF276_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX6605701.1"
                     /translation="MKVQVCVGDETFQVEVDDLHTRPIIARVAGEEFAVWPDSAPASA
                     ANGAAAPDHLVEPPALRLVPPPATHAAATPGGDGPAVRAPIPGVIHEILVREGERVEA
                     GQEICMLEAMKMQNTIRAPHAGRISRIPVAVGQAVKHRDVLLEFGD"
     gene            712..1839
                     /locus_tag="VF276_02195"
     CDS             712..1839
                     /locus_tag="VF276_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HEX6605702.1"
                     /translation="MNGSQLLADLLQGFTHFTAGNAVMILVGIVLILLAIGKEYEPML
                     LLPIGFGCLLANIPLTGMTDPDGLFSLLYKAGISTELFPLLIFVGIGAMIDFSPLLSQ
                     PRLVLLGAAGQFGIFGTLILATLLGFPLNEAASIGVIGAIDGPTSIFVATKLAPHLLA
                     PIAVAAYSYMSLIPIIQPPLMRLLTTPAERRIRMEYTPRPVSRRALVLFPIVITLILG
                     ILVPAAAPLVSTLMIGNLLRESHVVERLSKAAQNEIANIATLFLGLTIGSTMGAASFL
                     NGATLGILALGLVAFVLDTVAGLLFGKLMSVLSGRRINPLVGACGISAFPMAARLSAR
                     MANDEDNQNFILMQAIGANTAGQLASVVAGGVLLALVSGFL"
     gene            1876..>2256
                     /locus_tag="VF276_02200"
     CDS             1876..>2256
                     /locus_tag="VF276_02200"
                     /inference="COORDINATES: protein motif:HMM:NF015686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX6605703.1"
                     /translation="MESFVVRLMGIVVINLLLSGDNAVVIGMAVRGLPGALKRRAILI
                     GGAGALVLRVVLTGLAALLLQVPLLEAAGGLMLAWITYRLLIPSADAADPFDPDEHMT
                     FGLALRVIIIADLTMSLDNVLAVGA"
BASE COUNT          340 a          743 c          777 g          396 t
ORIGIN      
        1 gtgatcggcg gcctggtcta tctgctcgcc gatcgggagc aggagggcgc tgcaccactg
       61 gccgagccgg cgccgcccca ggaggtgggg gacgcggtcg cgcagcggcg taaggagcag
      121 gccgccgtcg cggcggtggc cgtggccctg gctctgacca gggaagccga agcggtgcac
      181 ccggcggtgc ggggcgcacc catcaccccc tggcaaaaca ttatgcgggg gagacaggca
      241 ctagagcgag ggcgaagacg atgaaagtcc aggtgtgtgt tggggacgaa acgttccagg
      301 tcgaagtcga cgacttgcac acgcgtccga taatcgcccg cgtggcgggc gaggagttcg
      361 ccgtctggcc ggatagcgcg ccggccagtg ccgcgaacgg cgccgccgca ccggaccacc
      421 tcgtggaacc gccggccctg cggctcgtac cgccgccggc gacacacgcg gcggcgacgc
      481 cgggcggaga tggtcccgcc gtgcgcgcgc ccatccccgg cgtcattcac gagattctgg
      541 tgcgcgaggg cgagcgcgtg gaggcgggcc aggagatctg tatgctggaa gcgatgaaga
      601 tgcagaacac catccgcgcc ccccatgccg gccgcatcag ccggatcccg gtggcggtgg
      661 gccaggcggt caaacaccgc gatgtcctgc tggagttcgg ggactgagcc gatgaacgga
      721 tcgcaactgc tggccgatct gctgcaaggg tttacgcatt tcacggcggg caacgccgtc
      781 atgatcctgg tgggcattgt gctgatcctg ctggcgatcg gcaaggaata cgagccgatg
      841 ctgctgctgc cgatcgggtt tggctgcctg ctggcgaata ttcccctgac cgggatgacc
      901 gaccccgacg gtctcttctc gctgttgtac aaggccggga tcagcaccga attgttcccc
      961 ctgctgatct tcgtcggcat cggggcgatg atcgacttca gcccgctgtt gagccagccg
     1021 cgcctggtgc tgctaggcgc cgccggccag tttggcatct tcggcacgct gatcctggcg
     1081 acgctgctgg gcttcccgct caacgaggcg gcctcgatcg gcgtgatcgg ggccatcgac
     1141 gggcccactt cgatctttgt ggcgacgaaa ctggcgccgc acctgctggc gccgattgcc
     1201 gtcgccgcct attcctacat gagcctgatc ccgatcatcc agccgccgtt gatgcgcctg
     1261 ctgaccaccc cagcagagcg gcgcatccgc atggagtata cgccacggcc cgtgtcgcgc
     1321 cgggcgctcg tcctcttccc catcgtcatc accctgatcc tcggcatcct ggtgcccgcc
     1381 gcggcgcccc tcgtcagcac cttgatgatc ggcaacctcc tgcgcgagag ccatgtggtc
     1441 gagcgcctga gcaaagcagc gcagaacgag attgccaata tcgccacgct gttcctcggg
     1501 ctgaccatcg gctcgacgat gggtgcggcc agtttcctca acggcgcgac gctgggtatc
     1561 ctggcgttgg gcctggtggc ctttgtgctg gacacggtgg ccggcctgct gttcggtaag
     1621 ctgatgtcgg tgctgagcgg tcgacggatc aacccgctcg tcggcgcgtg cgggatcagc
     1681 gcgttcccga tggcggcgcg gctctcggcg cgcatggcca acgacgaaga caaccagaac
     1741 tttatcctga tgcaggccat tggcgccaac accgccgggc aactcgccag cgtcgtcgcc
     1801 gggggggtgc tgttggccct ggtttccggg tttctatagt cccggcagag gcgcgccggc
     1861 aggaggcacg agatcgtgga gagttttgtg gtccgcctca tgggcatcgt cgtcatcaac
     1921 ctgctcctga gcggggataa cgccgtggtc atcggcatgg ccgtgcgcgg gctaccgggc
     1981 gcgctgaagc gccgggcaat cctcatcggc ggggccggcg cgctggtcct gcgggtggtg
     2041 ctcacgggcc tggctgcctt gctgctgcaa gtgcccctgc tcgaagcggc cggcggcctg
     2101 atgctcgcct ggatcaccta tcgcctgctg atcccgagtg ccgatgccgc cgacccgttc
     2161 gaccccgatg agcacatgac tttcggcctg gcgctccgcg tgatcatcat cgccgatctg
     2221 acgatgagcc tcgataacgt gctggcggtc ggagcg
//
LOCUS       DASUYC010000135         2093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_65042,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000135 DASUYC010000000
VERSION     DASUYC010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65042"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(5..81)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            326..907
                     /gene="scpB"
                     /locus_tag="VF276_02205"
     CDS             326..907
                     /gene="scpB"
                     /locus_tag="VF276_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX6605704.1"
                     /translation="MDETDSPSPEPRRDDEGIAELARAIEAVLFAAPEPQALDALRKG
                     LGASATRLDAALDHLAADLRAGRRGIRLQRLTNQVQLVTAPEMGPELERYFGYEAGTR
                     LSAAALETLAIIAYRQPVTRAQVEQLRGVDCSGVINTLLNRGLIAESGRLDTAGHPIL
                     FSTTLAFLRHFGLGSLSDLPPLPAEQMPQEPAG"
     gene            complement(941..1552)
                     /locus_tag="VF276_02210"
     CDS             complement(941..1552)
                     /locus_tag="VF276_02210"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX6605705.1"
                     /translation="MKADQFSAQMVEIMALPPEERHERMAVLHTNIVTEYRAAVEALT
                     DEQARQIVAGGADPRTLAQVVGHIAEWERFAILGAGDMLAGLTHPRMVTSVAGYYEPD
                     GTAPTFENIDAFNAYQAARHASWPWARIQALALETATTLHALFTEPRLLSAARLEATE
                     PFRKRLEDGTRIRNITMGWNLWITVLEHEAVEHAGALGMVGGV"
     gene            complement(1754..>2093)
                     /locus_tag="VF276_02215"
     CDS             complement(1754..>2093)
                     /locus_tag="VF276_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004842707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX6605706.1"
                     /translation="TERLPNNAAFCFAGCSGEDLLLMLDLAGICASSGSACNAGSTQP
                     SHVLTAIGTPLDLAHGSLRLTLGAENTEAEIDYTLDTLERIVHELRTHRPHGAAAESQ
                     PVPTVAAGAV"
BASE COUNT          343 a          714 c          695 g          341 t
ORIGIN      
        1 tgagttcggt aacccggcta tccagcaact ggacccgtgg ctttgcgtcc tcacctcgcg
       61 atgagtttgc cgtttcgctc cgcacgccgg cctgcccaag cctggtagag cccgcgtggt
      121 ttcattatag atgatgaacc ttacacgcca gcttaccgag ttggttacag tttcataaca
      181 atgcggctgg ataaagaaat atatcgcgcg aggctgccgc ccggcccgct caactgcgcc
      241 ctcgccaccg cgcggggtgg tagtgtataa taaggcgccc cgccgccccg cgccgggccg
      301 ggtagccttg ccggatggaa ttgtgatgga cgaaaccgac agccccagcc ccgaaccccg
      361 ccgcgacgac gaaggcatcg cggaactagc tcgcgccatc gaagccgtgc tgtttgccgc
      421 gcccgaaccc caggcgctcg atgcgctgcg caagggcctg ggcgcgtcgg cgacgcggct
      481 cgatgccgcg ctggatcacc tggccgccga tctgcgcgcc gggcgccgtg gcatccgcct
      541 gcagcggctg accaaccagg tccaactggt gacggcgccg gagatgggcc cggaactgga
      601 gcgctacttc ggctacgagg cgggcacgcg actgtctgcc gccgccctgg agaccctcgc
      661 catcatcgcc tatcgccagc cggtgacgcg ggcccaggtc gagcaattgc gcggcgtcga
      721 ttgtagcggc gtgatcaaca ccctgttgaa tcgcggcctg atcgccgaga gcggacgact
      781 ggataccgcc gggcacccca tcctcttcag caccacgctg gccttcctgc gccacttcgg
      841 tctcggcagc ctgagcgatc tgccgcccct ccccgccgag cagatgccgc aggaaccggc
      901 cgggtagcgg gccgcccgcc gcgccgcaac ggcgcacccg tcacacgccg cccaccatgc
      961 ccagcgcccc ggcgtgctcg acggcttcgt gttccagcac ggtaatccac agattccagc
     1021 ccatcgtgat attgcggatg cgggtgccgt cttccagccg cttgcggaag ggttcggtcg
     1081 cttccaggcg tgccgccgac agcaagcgcg gctcggtgaa gagcgcatgc agcgtggtgg
     1141 ccgtctccag cgccagcgcc tggatgcggg cccagggcca cgaggcgtgc cgggccgcct
     1201 ggtaggcgtt gaaggcatca atgttctcga acgtgggcgc cgtgccgtcg ggctcatagt
     1261 agcccgcgac gctcgtcacc atgcgtgggt gggtcagccc ggccagcata tcgcccgcgc
     1321 ccaggatcgc gaagcgctcc cactcggcga tgtggccgac cacctgggcc agggtgcgtg
     1381 ggtccgcccc gccggccacg atctgcctgg cctgctcgtc cgtcagcgct tccaccgccg
     1441 cccggtactc ggtgacgatg ttcgtgtgca gcacggccat acgctcgtgg cgctcctccg
     1501 ggggcagcgc catgatctcc accatctgcg cggaaaactg atcggctttc acaattgtct
     1561 ccttcgctgt ctgttggcaa agagccgggt tgttatgtca aggaagcagc acagtaagcc
     1621 acaaccaagg ccggttgcaa accggcccct acaagtaccc gtcgcagggt agagacgagg
     1681 caaaaacaaa gccaccagcc ggggcaagtg gctttgttct gattcaaggc cggctcgggc
     1741 cggtacgcgc cggctaaacg gcgcctgcgg ccacggtggg cacgggctgg gactcggcgg
     1801 ctgcgccgtg cgggcggtgg gtgcgcaact cgtggacgat gcgctccagg gtatcgaggg
     1861 tgtagtcgat ctcggcttcc gtattctccg cgccgagggt caggcggagg gaaccgtgag
     1921 ccaggtcgag cggcgtgccg atagcggtca gcacatggct gggctgggtg gacccggcgt
     1981 tacaggccga gccgctggag gcacagatgc cggccaggtc cagcatgagc aggagatctt
     2041 cgccgctaca gcccgcgaag cagaaggcgg cgttgttcgg cagacgctca gtc
//
LOCUS       DASUYC010000136         2709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1342692, whole genome shotgun sequence.
ACCESSION   DASUYC010000136 DASUYC010000000
VERSION     DASUYC010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1342692"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="VF276_02220"
     CDS             <1..971
                     /locus_tag="VF276_02220"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX6605707.1"
                     /translation="KCSVVDVPYGGGKGGVVVDPTKLSVGELERLSRRFITEIQGIIG
                     PERDIPAPDVNTNSQIMAWMMDQYSRNRGYPVPGMITGKPLALGGSLGRTDATGVGVM
                     MATRQAMQKLNMPAEGTRVVVQGFGNVGSVSARAMHELGTTIVAVSDVFGGIYNPKGL
                     DIPALLAHMRRTGTVKGFADADAITNEELFGIDCDILIPAALENQITAQNADKIKAKL
                     IAEGANGPTTPEADRILHDRGILVLPDILANAGGVTVSYFEWAQNIQGFYWTEEEVAE
                     RLERVMVRSFRAVYETAQEHEVDMRTAAYIVAIGRVAEAVRLRGIA"
     gene            1221..2525
                     /locus_tag="VF276_02225"
     CDS             1221..2525
                     /locus_tag="VF276_02225"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX6605708.1"
                     /translation="MVESIQVNVDQTIPTITPPRPPKPNPFLVAQEQIHIAAQRMKLD
                     PEMEAILRQPERELHVHFPIRMDDGSIRMFDGYRVQHNTARGPAKGGIRFHQDVSINE
                     VKALATWMTWKCSVVDIPYGGGKGGIIVDPKKLSLGELERLSRRFVTEIQGIIGPERD
                     IPAPDVNTNSQIMAWMMDQYSRNKGYPIPGMITGKPLALGGSVGRNEATARGLQFCVR
                     EAVRTTGMDLAGATVVIQGYGNAGNIAARLLMEDGARVVAVSDSHGGIYAPDGLDPVR
                     VLRWKEEHGSVQGFGNAGNVTNEELLEIPCDILVPAALEGQITAHNADKIKAKLIAEA
                     ANGPTTPEADRILYEKGVLLLPDILANAGGVTVSYFEWAQNIQGFYWTEEEVDERLHR
                     VMVRAFKAVYETAQEHEVDMRTAAYIVAIGRVAEAVRLRGIY"
BASE COUNT          516 a          906 c          865 g          422 t
ORIGIN      
        1 ggaaatgctc ggtcgtcgat gtgccctacg gcggcggcaa gggcggcgtg gtggtcgatc
       61 ccaccaagtt gtcggtgggc gagttggagc ggctgagccg gcggttcata accgagatcc
      121 aggggatcat cgggccggag cgcgacatcc cagcgccgga cgtgaacacc aacagccaga
      181 tcatggcctg gatgatggac cagtacagcc gcaaccgcgg ctaccccgtg cccggcatga
      241 tcaccggcaa acccctcgcc ctcggcggct cgctgggccg caccgatgcc accggcgtcg
      301 gcgtgatgat ggcgacccgc caggccatgc aaaagctgaa catgcccgcc gagggcaccc
      361 gcgtcgtggt gcagggcttc ggcaatgtcg gctcggtctc cgcccgcgcg atgcacgagc
      421 tcggcaccac catcgtcgcc gtgagcgacg tgttcggcgg catctataat cccaagggcc
      481 tcgacatccc ggccctgctc gcccatatgc gccggaccgg cacggtcaaa ggcttcgcgg
      541 atgccgatgc catcaccaac gaggaactct tcggcatcga ttgcgacatc ctgatcccgg
      601 cggcgctcga aaaccagatt accgcgcaga acgccgacaa aatcaaggcc aagctgatcg
      661 ccgagggcgc caacggcccg acgacacccg aagccgaccg catcctgcat gatcgcggca
      721 tcctggtgct gcccgacatc ctggccaatg ccggcggcgt gaccgtctcg tacttcgagt
      781 gggcccagaa catccagggc ttctactgga ccgaggaaga ggtcgccgag cggttggagc
      841 gcgtgatggt gcgctcgttc cgcgcggtgt acgagacggc ccaggagcac gaggtcgata
      901 tgcgcaccgc cgcctatatc gtcgccatcg ggcgcgtggc cgaggccgtc cgcctgcgcg
      961 gcatcgccta agcgcggatc gacgagcgct gatcccggta ggagccggct tgccgccggc
     1021 tcctactcta tttcccccca acccgttcct cgatcttact gctttgtaag ggtttaatga
     1081 gaccgtctgt gctataatag cactggaaac ccctatttcc ctgctagttg gcgcaccgac
     1141 gccggcttta ccagcggccc aagctggcgc ctgggagatt cctcgctatt cgcagttgaa
     1201 gaagaggagg acagcattcc atggtggaaa gcatacaggt caacgttgac cagacgatcc
     1261 ccacgattac ccccccgcgc ccacccaaac ccaacccatt cttagtcgcc caggaacaga
     1321 tccatattgc cgcccagcgg atgaagctcg atcccgagat ggaagcgatt ctgcgccagc
     1381 ccgagcgcga actgcatgtc catttcccga tccgcatgga cgacggcagc attcgcatgt
     1441 tcgacggcta ccgcgtgcag cacaacaccg cccgcggccc ggccaagggc ggcattcgct
     1501 tccaccagga tgtcagcatc aacgaggtca aggccctcgc cacctggatg acctggaaat
     1561 gctcggtcgt ggacatcccg tacggcggcg gcaaaggtgg gatcatcgtg gatcccaaga
     1621 aactctcgct gggcgagttg gagcggctga gccggcggtt cgtgaccgag atccagggga
     1681 tcatcgggcc ggagcgcgac atcccggcgc cggacgtgaa caccaacagc cagatcatgg
     1741 cctggatgat ggaccagtac agccgcaaca agggctaccc catccccggc atgatcaccg
     1801 gtaaacccct cgccctcggc ggctccgtcg ggcgcaacga ggcgaccgcg cggggcctgc
     1861 agttctgcgt gcgcgaagcg gtgcgcacca ccggtatgga cctcgccggc gcgacggtgg
     1921 tcatccaggg ctacgggaac gccgggaata tcgccgcccg gctgctgatg gaggacggcg
     1981 ccagggtcgt cgccgtcagc gacagccacg gcggcattta tgccccggat ggcctggatc
     2041 cggtgcgcgt gctgcgctgg aaagaagagc acggctcggt gcaggggttc ggcaacgccg
     2101 gcaatgtcac caacgaagaa ctgctggaga tcccctgcga catcctggtg ccggcggcgc
     2161 tcgaaggcca gatcaccgcg cacaacgccg acaaaatcaa ggccaaactg atcgccgagg
     2221 ccgccaacgg cccgacgaca cccgaagccg accgtatctt gtacgaaaaa ggcgtcctct
     2281 tgctgcccga catcctggcg aatgccggcg gcgtgaccgt ctcgtacttc gagtgggccc
     2341 agaacatcca gggcttctac tggacggaag aagaagtcga cgaacggctg caccgggtaa
     2401 tggtgcgggc gttcaaagcc gtgtacgaga cggcccagga gcacgaggtc gatatgcgga
     2461 ccgccgccta tatcgtcgcc atcggtcggg tggccgaggc cgtccgcctg cgcggcatct
     2521 actgaccggc gcgccgcccg gcacccgccg ggcggcctct cgcatgtaca cacgtccctc
     2581 gtcgcccggc ggcgggggac atttcgttgt gccgggcggc tcctgggtac cgggccgccc
     2641 gccgggtaga gggcatgaaa cgtgctttta tgctcgcaga ggccgtggca acggggcgcc
     2701 atggtgtgc
//
LOCUS       DASUYC010000137         2991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1681518, whole genome shotgun sequence.
ACCESSION   DASUYC010000137 DASUYC010000000
VERSION     DASUYC010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1681518"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1796)
                     /locus_tag="VF276_02230"
     CDS             complement(<1..1796)
                     /locus_tag="VF276_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX6605709.1"
                     /translation="MPVPDSALSGPIPCPRCGRVLEPGQKFCDGCGLDLWEPTVPTPL
                     VLVAAAPPAQPPQRKRTRKAPAVPDAAPAAEAATHRLPDAPADAPVEAPPQRKRTRKA
                     PAAPAAPAAPVAGAAALFLPDAALPAEAPAAPEAPPAPPKRKRTRKPPAAQATPAPAP
                     EVPPAPSADWLAEPPAQSPVPPKPRRPRRAAPTVAPAPEAPPEAGTQVQPEAPPERGT
                     QFLPEAPSERGTQFLSEVPPEPGTQFLPEVAPALGTQFLPEVPPPAPAADAAPPAPAV
                     TRHLEVAAPVAPYLPPASVAPAKPRRPRRRRRAAAPAPAPAPAPAPAPAAPHILPHSA
                     RRTLPGGDQTALVGIVLLGVLAIIVFSQAARGVFGPVPALSPTPAPVPATPPRLNVVP
                     PTLALGPLTPTPDGAVANLDIVASTTYRDRAGTLCLVGVIGNGLDVGLGAEVTATLVD
                     SAGATLAQAQSRYYPGSLLAPGVRLPFRMELAHPPPAWGNIQWSLSAFDRSGESEIVY
                     HSLEAQDLELRPPAGPTAPWMLAGQIANPGARAARPVRLYAAVYDAAGALLDANEVDG
                     PPDSVVPGARTAFTLPLPRPGMQPTRAEVVVEG"
     gene            complement(1830..2840)
                     /locus_tag="VF276_02235"
     CDS             complement(1830..2840)
                     /locus_tag="VF276_02235"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605710.1"
                     /translation="MGERKAWISAPAIAGLIILVLAVVVVVRAVVLQISPPSPVRTLV
                     EYGFCLRCSTPPGVFDATFFPDGETMALGLSTATEVRRVANGELLQTLPSGPTFHVAV
                     SPDGQLVATVAEHAVQLWRTTDGVSLWTVSTPARDTALAFSPDSRLLLAAGESGIEVR
                     RIADGSLADTVPGWARDLALLPDGLLASAGVDTIRIWSFAQAGGRLSLPPIPMGTVPG
                     WVYAAISPDGQLVAAPVSASASDVQIWHWRDGQPRVRLRPQSDDAQVHGVSFAPDGQT
                     LATVSAGSSNTLRLWRVTDGIEIARQKIDSSSSIEGGIFAPDGKTLAAWSVSAVYLWT
                     LP"
BASE COUNT          409 a         1039 c         1152 g          391 t
ORIGIN      
        1 ccttcgacga cgacctcggc gcgggtgggt tgcatgccgg ggcgcggcag gggcagggtg
       61 aacgccgtgc gcgcgccggg gaccaccgaa tcgggcgggc cgtcgacttc gttggcgtcg
      121 agcagggcgc ccgcggcatc gtagacggcg gcgtagaggc gcaccgggcg ggcggcgcgg
      181 gcgccggggt tggcgatctg cccggccagc atccagggcg ccgtgggccc ggcgggcggg
      241 cgcaactcca gatcctgcgc ctctaggctg tggtagacga tctcggactc gcccgaccgg
      301 tcgaaggcgc tcaggctcca ttggatgttg ccccaggcgg gcggtgggtg ggccagttcc
      361 atccggaagg gcaggcgcac gccgggcgcc agcaggctgc cggggtagta gcgcgactgc
      421 gcctgcgcca gcgtggcgcc cgcgctatcg accagggtcg ccgtcacctc ggcgcccagg
      481 cccacgtcca ggccgttgcc gataacgccg accaggcaga gcgtgcccgc ccggtcgcgg
      541 taggtggtcg aggcgacgat gtcgaggttg gcgacggcgc cgtcgggcgt gggcgtgagc
      601 gggccgagcg ccagggtggg cgggaccacg ttgaggcgcg gcggggtggc cggcacgggc
      661 gcgggcgtgg ggctgagggc cgggaccggc ccgaagacgc cccgcgccgc ctggctgaac
      721 acgataatcg ccagcacccc cagcagcacg atccccacga gcgccgtctg gtcgccgccc
      781 ggcagcgtgc gccgcgcgct gtgcggcagg atatgcggcg ccgcaggggc gggcgcgggt
      841 gcgggcgccg gcgccggcgc gggcgcggcg gctcgccggc ggcggcgcgg acggcgcggc
      901 ttcgccgggg ccaccgacgc gggcggcaga tagggtgcga cgggtgcggc cacctccagg
      961 tgccgggtga cggctggggc gggcggggcc gcgtcagcgg caggcgcggg cggcggcacc
     1021 tcgggcagga actgcgtacc tagcgcgggc gctacctcgg gcaggaactg cgtgcctggc
     1081 tcgggcggca cctcggacag gaactgggtg ccccgctcgg acggcgcctc gggcaggaac
     1141 tgggtgcccc gctcgggcgg cgcctccggc tggacctggg tccccgcctc gggcggcgcc
     1201 tccggggccg gggccactgt cggggctgcc ctgcgcgggc gccggggctt cggcggcacg
     1261 ggcgactggg caggcggttc cgccagccag tccgcggacg gcgcgggcgg cacttccggc
     1321 gccggggccg gggttgcttg cgcagcgggc ggcttgcgcg tgcgcttgcg cttcggcggc
     1381 gcgggtggtg cctcgggcgc ggcgggcgcc tccgcgggca gcgcggcgtc gggcaggaag
     1441 agcgccgccg cgccggcgac cggggccgcc ggggccgccg gcgcggcggg cgccttgcgg
     1501 gtacgcttgc gctgcggcgg cgcctcgacg ggcgcatcgg cgggcgcatc cggcagccga
     1561 tgcgtggccg cttcggcggc gggggcggca tccggcacgg cgggcgcctt acgggtgcgc
     1621 ttgcgctgcg gcggctgtgc gggcggggca gccgccacca gcacgagcgg cgtggggacc
     1681 gtcggctccc acaggtcgag cccgcagccg tcgcagaact tctgccccgg ttcgagaacc
     1741 cgcccgcagc gggggcaggg gatcgggccg ctcagggccg agtcaggcac gggcatgggc
     1801 accgctttcc aggaagcgac cagggccgtt cagggtagag tccataagta cacggcgctt
     1861 accgaccatg ccgctagggt tttgccgtcg ggagcaaaga tcccgccttc gatactactg
     1921 ctggaatcga tcttttgcct cgcgatttct atcccgtccg tcactcgcca cagccggaga
     1981 gtgttgctgg atcccgcgga aaccgtagct agcgtctgcc catcgggtgc aaagctgacg
     2041 ccgtgaactt gggcgtcgtc ggattgcggg cgcagccgga cgcgcggctg cccatcccgc
     2101 caatgccaga tctgcacatc gcttgccgac gccgataccg gcgcggccac cagttgccca
     2161 tcgggggaga tagcggcgta aacccagcca gggacggtac ccatcgggat tgggggcagc
     2221 gacaggcgcc ctcccgcttg agcgaaagac catatccgga tcgtgtctac gccggcactt
     2281 gccagcaaac catcgggcag caaagccaga tcccgcgccc agcccggcac cgtgtcggcc
     2341 agggatccat cagcgatgcg ccgcacttcg atgcccgatt cacccgccgc cagcagcagc
     2401 cggctatcgg gcgaaaaggc aagtgcggtg tctctcgccg gcgtactgac agtccagagc
     2461 gaaacgccgt ccgtcgtgcg ccatagctgc acagcgtgtt ccgccaccgt cgccacgagt
     2521 tgcccatccg ggctgaccgc gacgtggaat gtgggaccgc tcggcagggt ctgcagaagt
     2581 tcgccgttgg cgacccggcg gacctcggtg gccgtgctta gccccaacgc catcgtttct
     2641 ccatcgggga aaaaggtggc atcgaagacc cccggcggtg tggaacagcg caggcaaaac
     2701 ccgtattcca ccagggtccg caccgggctc ggcgggctga tttgcagcac gaccgcacga
     2761 acgactacaa cgacggcgag caccaggata atcagtcccg cgatggccgg ggcggaaatc
     2821 cacgctttgc gttcacccat gccggtgtct cactgttctg attcctccgt cccccgattc
     2881 gcgccaccgc ccggcgcaat acgacgcagg tgtatcacat catagctgct attataagcc
     2941 acaaccgcgc ggcgggccaa cctctccccg cccctccccg ccgcggggag g
//
LOCUS       DASUYC010000138         5305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1014044, whole genome shotgun sequence.
ACCESSION   DASUYC010000138 DASUYC010000000
VERSION     DASUYC010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5305
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014044"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VF276_02240"
     CDS             <1..526
                     /locus_tag="VF276_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605711.1"
                     /translation="TQDSMEPSQRAKHAGTGSLEKIGLLCELDWLVSLCVSLLAHHSA
                     VVKVLAWFYRSRERQGVLYQMEKPCVKALFHNLSTDLSATLSTELWITGAAGRQAHGH
                     TKTGAYNAGRTITVMLIRVPRLAVPLRSIFSARPNETGIGAAFHSGFGIIPDRVHPVK
                     GFRRRFCYQFCGRC"
     gene            812..1825
                     /locus_tag="VF276_02245"
     CDS             812..1825
                     /locus_tag="VF276_02245"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6605712.1"
                     /translation="MMDELQDRSEGQWRPDGPLPPITRLELRTPFKMGPVNSYVVDED
                     PLTLVDCGPLTEDAWADLQAGLARLGRRPEEIRRLILTHGHVDHWGLAARIVALSGAE
                     VWVHHRLDLWMREFADEWQRRMAYLTLLCIEGGVPSGEMVAINRGVKAMGGYAEAVPP
                     TRLLDAGDVLSLAGHEWTIHYTPGHATGHISLHQPETRTFIAGDHLLADISSNPVLEA
                     PLIGESARPRMLPLYVESLARVAELPVLWVFPGHGSPFQGHRKLIKRRLQLHEERAAR
                     LRDWLREEPAAVHTLSRRLFPRLEGVDLFLGFSETLGHLDLLEDRGQVRRDAARHPVV
                     YHV"
     gene            complement(1816..2697)
                     /locus_tag="VF276_02250"
     CDS             complement(1816..2697)
                     /locus_tag="VF276_02250"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6605713.1"
                     /translation="MNSITQTKKGPLEYRVVGQGPAVLVLNGGHTNCNSPFGHEQFFL
                     DQGCRLVIPSRPGYGRTPSATGKTAEMFADALVALLNRLRLERVIVVGISAGGPTALQ
                     LAGRHPERVSKLILQNAVTGGKFPAPGIRLGAYLMFNPWVERGTWAAFRAFARVAPMA
                     ALRSMMGSLSSLDPAQVVATMSREQQQAALAFLLASRSGSGFLHDLQHRCGDLRRITA
                     PTLIIESKYDGSVDASHAAYAADHIADAELFVSPAESHLLWFSSYNAAIEEKMRAFLT
                     AQHLARKGWPCPTACYT"
     gene            2837..3568
                     /locus_tag="VF276_02255"
     CDS             2837..3568
                     /locus_tag="VF276_02255"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX6605714.1"
                     /translation="MDPFANGSGPVPPGTRRAEGGANTAPLFPHDLPPEPGTEMTALR
                     RLPGPVLSGSTSYFPPSAVLSLLNLQKATGVLYLQNRALAATIGLEDGEVVAATLGSD
                     QGLPALFYALSWNTGTFEFRRVPPGPRSINLTLPVIQVRATLWLDRWKELRRFFPTIW
                     HRVGIHPQPAGEVVIQPHQWQVLTRIVAEPISLVRLAELLRMDVLAVTRVAVELVMLG
                     LAIVVAPDEGEAERDAAAEEWNLGL"
     gene            3558..4025
                     /locus_tag="VF276_02260"
     CDS             3558..4025
                     /locus_tag="VF276_02260"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6605715.1"
                     /translation="MASDLPADAPPDSAGAASRSLRRVGVIDDDYMTARLVCTLLELD
                     GYQATHFPNSSSLPDDLVQAGVGAVICDNRMPGRSGSEVLALLRADPRFREVRAVLMS
                     GLPLDETAWRDQGADAFLLKPFTHRHLLAALTAAGAGPPPPRRSGAGQEERNG"
     gene            4022..5209
                     /locus_tag="VF276_02265"
     CDS             4022..5209
                     /locus_tag="VF276_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HEX6605716.1"
                     /translation="MSLDTDLRGAGRMAARVAGFGTSIFSEMSALARAHGAVNLSQGF
                     PDFPSPEWIRAAAAQAIAADLNQYAVSHGSPRLRQALAAQEAAAYGLAYDPDREVTVT
                     SGATEAIFAAMLALVNPGDEVIVFEPCYDSYVPSIEMAGGTARYVRLDAPGWEFDPAA
                     LEAAVTPRTRLILLNTPHNPTGKVFTPIEMETVADLCRRHNLIAVTDEVYEHIVFPPA
                     THVPLATLPGMRDRTVTISSTSKTFSLTGWKIGYAFAPPDLTEAIRRVHQFVVFCSVT
                     PLQEAMATAVEEAPGRGYYAEFLAAYATRRDFLYHALRKAGLRPQQPQGAYFIMCDVT
                     HLGFADDVAFARYLTTEVGVAAIPPSAFYHTPPPQQLARFCFAKNPQTLQAAADRLAA
                     WRR"
BASE COUNT          884 a         1739 c         1730 g          952 t
ORIGIN      
        1 cacccaagat tcgatggaac catctcaacg tgcgaagcac gccggaaccg gttcgttgga
       61 aaagattggg ctattgtgtg aacttgactg gttggtgtca ctttgtgtga gcttgcttgc
      121 tcaccactct gctgttgtta aagtgcttgc ttggttctac cgctctcgcg agcggcaagg
      181 ggtattgtac cagatggaga agccctgtgt caaggcgctt tttcacaact tgtccaccga
      241 tttatccgca actttatcca ccgaattgtg gataacgggg gcggccggca gacaagccca
      301 cgggcacaca aaaaccggcg cttacaacgc cggccggacg attacagtga tgctgatccg
      361 tgtcccgaga ctggctgttc ccttgagatc gatcttttcg gcaaggccga acgagaccgg
      421 aataggtgcc gcgtttcaca gcggctttgg tattataccc gatcgtgtcc accctgtcaa
      481 ggggtttcgc cgtcgatttt gctaccaatt ttgcggccgc tgttagattg ggcagggtcg
      541 tttcggcggg gccgaacgag atcgtgaatt gcgccggttt cgcaaccggc ttactgatta
      601 taccgagcgg gcgggctcat gtcaaggaat ccgggcacaa gttttagatc catttccgac
      661 aattatcatc caagcccaca acacagcggc accgcaggcc ggcggcacga ccggcgggcg
      721 cggggcgcgg cgcggtttga cattcgcgga cggcagtttt atgatgaatt gcttgcggtg
      781 gctaccgccg gttgaggagg atcgcggatc gatgatggac gagttacagg atcggagcga
      841 gggccagtgg cgcccggatg gaccgctgcc gccgattacc cggctggaac tgcgcacccc
      901 gttcaagatg gggccggtca acagctatgt ggtagacgag gacccgctga cgctggtgga
      961 ttgcggacca ctgaccgagg atgcctgggc cgacttgcag gccgggctgg cgcgcctggg
     1021 ccggcgcccc gaggagattc gccggctcat tctgacgcac ggccacgtcg accactgggg
     1081 gctggcggcg cggatcgtgg ccctaagcgg ggccgaagtc tgggtgcacc accggctgga
     1141 cctgtggatg cgcgagttcg ccgacgagtg gcaacggcgc atggcctatt tgacgctgct
     1201 gtgtatcgag ggcggcgtgc cgtcgggcga gatggtggcg attaaccgcg gcgtcaaggc
     1261 gatgggcggc tatgccgagg cggtgccgcc cacacgcctg ctcgacgcgg gcgacgtgct
     1321 ctcgctggcc ggccatgagt ggaccatcca ctataccccc ggccacgcca ccggccatat
     1381 ctcgctgcac cagccggaga cccgcacctt catcgccggc gaccacctgc tggccgatat
     1441 cagctcgaac ccggtgctcg aagcgccgct gatcggcgag agcgcgcgcc cgcgtatgct
     1501 gccgctctac gtcgagtcgc tggcgcgggt ggctgagttg ccggtgctgt gggtctttcc
     1561 gggccacggc agcccgttcc aggggcaccg caaactgatc aagcgccggt tgcaactgca
     1621 tgaagagcgc gcggcccggc tgcgcgactg gctgcgcgag gagccggccg ctgtccacac
     1681 gctgagtcgc cgcctcttcc cgcggctcga aggggtcgat ctgttcctcg gcttctccga
     1741 aaccctcggc catctcgatt tgctggagga ccgcggccag gtccgccggg acgcggctcg
     1801 ccatcctgtt gtgtatcatg tgtagcaagc agtaggacag ggccagccct tccgagcgag
     1861 gtgctgtgct gttaagaacg cccgcatttt ctcttcaatg gcagcgttgt acgagctaaa
     1921 ccacagcaga tgactctcgg ctggagagac aaagagttcc gcatcggcaa tatggtctgc
     1981 cgcataggcg gcgtgagagg cgtccacaga tccgtcatac ttgctctcga taatcaaggt
     2041 gggcgccgta atacgccgga gatcgccgca ccggtgctgc aagtcatgca gaaagccgga
     2101 tccggatcgc gacgccagca agaaagccag ggccgcttgc tgttgttccc gactcatggt
     2161 cgccaccacc tgcgcgggat ctaaagacga aagactgccc atcatacttc gcaaagcggc
     2221 catcggcgcg actcgcgcga aggcacgaaa tgcggcccag gtccctcgtt ccacccaggg
     2281 gttgaacata aggtaggcac caagccggat gccgggcgca gggaatttcc cgcctgtgac
     2341 ggcgttttgc aggatgagct tgctcacccg ttcaggatgg cgccccgcca attgtaaggc
     2401 tgtggggccg cccgccgaaa tgcccactac gattacccgc tcaaggcgca aacgattgag
     2461 gagcgcgacc agcgcatcgg caaacatttc ggcggttttg ccggtagcag acggcgttct
     2521 tccatagccg ggccgtgagg gtataaccag ccggcagccc tgatccagga aaaactgctc
     2581 atgtccaaat ggggagttgc agttggtatg tccaccattg agcacgagca ccgccggccc
     2641 ttgccccacg acgcgatatt caagcggtcc cttcttcgtc tgagtaatgc tgttcattag
     2701 cgttcctctt tccctaagct gcacccggat cattccgtcc ctggcgggag cctacccgct
     2761 tcctatatgc tataatagaa ggacacttgg gcgccaggga tactacacgc ctgatagctg
     2821 acgaaggagc gtgcgcgtgg atccgtttgc gaatgggagc ggcccggttc cccccggcac
     2881 ccgccgggcc gaagggggcg ccaataccgc accgctgttt ccgcacgatc tgccccccga
     2941 acccggcacc gagatgaccg cgttgcgccg cctgcccggc ccggtgttga gcgggtccac
     3001 cagctatttc ccgcccagcg ctgtcctgtc gctgctgaac ctccagaaag ccaccggggt
     3061 gctctatctg cagaaccgcg cgctggcggc cacaatcggc ctcgaagacg gggaggtcgt
     3121 ggcggccacg ctgggcagtg accagggctt gccggcgctg ttctatgcgc tgagttggaa
     3181 caccggcacg ttcgaatttc gccgcgtgcc gcccgggccg cgcagcatca acctgacctt
     3241 gcccgtgatc caggtgcgcg ccacgctctg gctcgaccgc tggaaggaac tgcgccgctt
     3301 cttcccgacc atctggcacc gggtggggat tcacccgcag ccggcgggcg aagtggtgat
     3361 ccagccgcat caatggcagg tactgacgcg cattgtggcc gaaccgatca gcctggtgcg
     3421 cctcgcggag ttgctgcgca tggatgtgct agcggtgacg cgcgtggccg tggaactggt
     3481 gatgctgggg ctggcgattg tggtggcgcc cgatgaaggg gaggcggagc gcgatgccgc
     3541 cgccgaggag tggaatcttg gcctctgatc tcccggcgga tgcgccaccg gactctgccg
     3601 gtgcggcgtc tcgctcgctg cgccgcgtcg gcgtgatcga cgacgattat atgacggcgc
     3661 gcctggtctg caccctgctg gaactggacg gctaccaggc tacccacttc cccaacagct
     3721 ccagcctgcc cgatgatctg gtgcaggcgg gcgtgggcgc ggtcatctgc gataaccgca
     3781 tgccgggtcg cagcggctcg gaggtgctgg cgctgttgcg cgccgatccc cgcttccgcg
     3841 aggtgcgcgc cgtgctgatg tcgggcctgc cgctggatga gaccgcctgg cgcgaccagg
     3901 gcgccgatgc cttcctgctc aagcctttta cccatcgcca tttgctggcc gcgctgaccg
     3961 ctgccggcgc cgggccgccg ccgccccgcc gctcgggcgc cggtcaggaa gaaaggaacg
     4021 gatgagcctc gataccgatc tgcggggcgc cgggcgcatg gccgcgcgtg tggccggctt
     4081 cggcacctcg atcttcagcg aaatgagcgc gctggcccgc gcgcatggcg ccgtgaacct
     4141 gtcgcagggc ttccccgatt ttccctcgcc ggagtggatc cgcgccgccg ccgcgcaggc
     4201 catcgccgcc gacctgaacc agtatgccgt cagccacggg tcgccgcggc tgcgccaggc
     4261 gctggctgcc caggaagccg ccgcctatgg cctggcctac gacccggacc gcgaagtcac
     4321 cgtgaccagc ggcgcgacgg aggcgatctt cgcggcgatg ctggccctgg tgaaccccgg
     4381 cgacgaggtg atcgtgttcg agccgtgcta cgactcctac gtgcccagta tcgagatggc
     4441 cgggggcacc gcgcgctacg tccgcctgga cgcgcccggc tgggagttcg acccggcggc
     4501 gctcgaagcc gccgtcacgc cgcgcacgcg cctgatcctg ctcaacacgc cgcataatcc
     4561 caccggcaag gtcttcacgc ccatcgagat ggagacggtg gcagatctct gccgccggca
     4621 caacctcatt gccgtgaccg acgaggtcta cgagcatatc gtcttcccgc ccgccacgca
     4681 tgtgccgctg gcgaccctgc ccggcatgcg cgacaggacg gtgacgatca gcagcacctc
     4741 gaagaccttc agcctcaccg gctggaagat cggctatgcc ttcgcgccgc ccgacctgac
     4801 cgaggccatc cgccgcgtac accagtttgt cgtcttttgc tcggtgacgc cgttgcagga
     4861 ggcgatggcg acggctgtcg aagaggcgcc ggggcgcggg tattacgccg agttcctggc
     4921 cgcctatgcc acgcggcgcg acttcctcta ccacgcgctg cgcaaggccg gcctgcgccc
     4981 gcagcaaccg cagggcgcct acttcatcat gtgcgacgtg acccatctgg gcttcgccga
     5041 cgatgtggcc tttgcccgct acctcaccac ggaggtcggc gtggcggcca ttcccccgtc
     5101 ggccttctac cacacgccgc cgccccagca gttggcccgc ttctgcttcg ccaagaatcc
     5161 gcaaaccctg caagccgccg ccgaccgcct cgccgcctgg cgtcgctagg tcagcggcgc
     5221 gtaggcgtga ggggccggtg atccggcttc tcgcgaaccg gcgcgggcca ggccgggctg
     5281 gcgaagatgg tgcgggtggg agtgc
//
LOCUS       DASUYC010000139         2159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1019936, whole genome shotgun sequence.
ACCESSION   DASUYC010000139 DASUYC010000000
VERSION     DASUYC010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1019936"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..1408)
                     /locus_tag="VF276_02270"
     CDS             complement(113..1408)
                     /locus_tag="VF276_02270"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007390473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX6605717.1"
                     /translation="MAAAPDSSSLHATGDAPPHEGVQPATDSPSRAARFGRYARPTPG
                     GHSPVLAAPTRPARVSGRQVRAFVRNTLVLAVIATLALLLGLSWQRSTALEAEVAALS
                     HDAEVSRTHESELNTVLRLQKDKLSGQDAQIQSTGQDMQTVQRELQDLRDRMARLDQH
                     NAELRGALGLTEEDGAITASPTPTTTLTPGPTSLAPGPEGGESGPLYTLLLAPPLATP
                     NPNPPDLIANFRDSLADLNHSLDIYEQIEDELTNVAAQRMQEWAQMDEDDDTEAPPQV
                     AEDKPPPPADDGGAGKPPVKKPPAAVSHVPSGYPYYGLITSPFGWRSSPFVQGKVGFH
                     KGLDIKAPMGTPVHATQTGIVSQAGWSDVYGRMVMIDHGAGWSTLYGHNSKLLVSVGQ
                     RVVKGQVIAYSGSTGMSTGPHIHYEVRRNGVPVNPANYR"
     gene            complement(1519..>2159)
                     /locus_tag="VF276_02275"
     CDS             complement(1519..>2159)
                     /locus_tag="VF276_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605718.1"
                     /translation="LIAVLDEPEHGWRVCDWYWRNGVALAPGVQVTDSEDARLGDLTA
                     PRQIYWLLAADDPGALARIAAGPPPGTQVAGQAERVLILREANPGGAAPAARLAALVQ
                     AVGAAMPGPRPRYLAAALGSAAQAQGDVAGARRWDRQAGTDRPLGAEHLANATGWVDR
                     GAPDRALIDIATARALEPANPAIHLRAAALLGQMGYAPAAERERRLAALLPP"
BASE COUNT          278 a          721 c          822 g          338 t
ORIGIN      
        1 ctggtgccgg gtagcaccgg atgtccgccg ctcaggcgca gtgatattgc cgggcggcaa
       61 ggccggtttg caaccggccc ctacaccgct cgacggtggt agctggtgcg ggctagcgat
      121 aattcgccgg gttgaccggc acgccgttgc ggcgcacttc gtagtggatg tgcgggccgg
      181 tggacatgcc cgtgctgccg gagtaggcga tgacctggcc tttgacgacg cgctggccca
      241 ccgagaccag cagcttcgag ttgtggccgt agagcgtgga ccaccccgcg ccgtggtcga
      301 tcatcaccat gcgcccgtac acgtcggacc acccggcctg gctcacgata ccggtctgcg
      361 tggcgtgcac cggcgtgccc atcggcgcct tgatgtccaa ccccttgtgg aagcccactt
      421 tgccctggac gaaggggctg ctgcgccacc cgaagggcga cgtgatcagg ccgtagtagg
      481 gatagccgct gggcacgtgc gacacggcgg cgggcggctt cttgacgggc ggcttgccgg
      541 caccaccgtc gtcggcgggg ggtgggggct tatcctcggc cacctgcggc ggggcttcgg
      601 tgtcgtcgtc ctcatccatc tgcgcccatt cttgcatccg ttgcgcggcc acgttagtca
      661 gttcatcctc gatctgctcg taaatatcga ggctgtggtt gaggtcggcc agcgagtcgc
      721 ggaaattggc gatgaggtcg ggcggattgg gattgggcgt cgccaggggc ggggcgagca
      781 gcagcgtgta gagcgggccg gattccccgc cctcggggcc gggggccagg ctggtggggc
      841 cgggcgtgag cgtcgtggtg ggcgtggggc tggcggtgat ggcgccgtct tcctcggtca
      901 ggccgagcgc gccgcgcaac tcggcgttgt gctggtcgag ccgggccatg cggtcgcgca
      961 ggtcctgcaa ttcgcgctgc accgtctgca tatcctggcc ggtgctctgg atctgcgcgt
     1021 cctggccgct cagcttgtcc ttctgcaggc gcagcacggt gttgagttcg ctctcgtgcg
     1081 tccggctgac ctcggcgtcg tggctcagcg cggcgacctc cgcttccagg gcggtagatc
     1141 gttgccaact cagccccagc agcagcgcca gggtggcgat cacggccagc acgagcgtgt
     1201 tgcggacgaa ggcgcgcacc tggcgtccgg aaacgcgggc agggcgggtc ggcgccgcca
     1261 gcaccggcga atggccgccg ggggtggggc gagcgtagcg gccaaagcgg gccgcgcggg
     1321 atggcgaatc agtagccggc tggactccct catgaggcgg cgcgtcacca gtcgcgtgga
     1381 gggaggaact gtcgggggcg gctgccacgt tatgtcatcc tcctgagcac aacgaaatag
     1441 tagcatagcc gccgagtagt gtcaataacc aaatagtcct atttcttacc agcccggtaa
     1501 ggcggcgcgg cggggaaact acggtggcag cagcgccgcc agtcgccgtt cgcgctccgc
     1561 tgccggcgca tagcccatct ggccgagcag cgcggcggcg cgtaggtgga tcgccgggtt
     1621 ggccggttcg agggcgcgtg cggtggcgat gtcgatcagc gcgcggtcag gcgcgccccg
     1681 gtccacccag ccggtggcgt tcgccaggtg ctcggcgccg aggggccggt cggtgcccgc
     1741 ctgccggtcc cagcgccgcg cgccggccac atcgccctgc gcctgggccg cgctgcccag
     1801 cgcggcggcc aggtagcggg gccgcggccc cggcatggcc gcgccgacgg cctggaccag
     1861 cgccgccagc cgggcggcgg gggcggcgcc gccggggttg gcctcgcgca agatcagcac
     1921 gcgctcggcc tggcccgcga cctgggtgcc gggcggcggc cccgccgcga tgcgcgccag
     1981 ggcgcccgga tcatcggcgg ccagcagcca gtagatctgc cggggcgcgg tcaggtcgcc
     2041 caggcgcgcg tcctccgagt ccgtcacctg gacgcccggc gcgagcgcga cgccgttgcg
     2101 ccagtaccag tcgcagacgc gccagccgtg ctccggctcg tcgagcacgg cgatcaggt
//
LOCUS       DASUYC010000140         2116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1450671, whole genome shotgun sequence.
ACCESSION   DASUYC010000140 DASUYC010000000
VERSION     DASUYC010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450671"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2116
                     /locus_tag="VF276_02280"
     CDS             <1..>2116
                     /locus_tag="VF276_02280"
                     /inference="COORDINATES: protein motif:HMM:NF016563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="non-lysosomal glucosylceramidase"
                     /protein_id="HEX6605719.1"
                     /translation="NGDDGPWQGAPVGGLGAGSIGRTYRGDFARWHLAPGENRYGTVW
                     ADQFSVRVARPEAPVSARVLLPARPPQGLDAWNWDYPADGGTYAALYPFAWYDYGAAA
                     AGVTLQARQFSPVIPHNYQESSYPVAVFEWTVTNDAATPADVSLLFTWENPLGWDDRV
                     ARPAGNTNRIRRDTLDGAPMLGIVMGRSAPDGTPGPTAVKQAWDGQFVIAARETPGVQ
                     LSALGRFATQGDGAEVWQPWAATGRLPDSDDPAPARQGEPIGAALAASTHLAPGESRT
                     ITFVLAWDLPLMQFGAGAKAEQWYRRYTEWFGKDGHHAWEIARTGLQQAPAWAAALTA
                     WQAPILAGTQRPTWYKTALFNELYYLVDGGTIWENGRVGDPTPSTHRFSYLECYDYPF
                     YSTLDVRFYGSFAQLMLWPEIEKAEMREFALTIPQSDPTRYITTDVEHRREAVRKVPG
                     AAPHDVGMPSEAPWTRINAYNYQDVSNWKDLNSKFVLLVYRDYIATGDRAFLDATWPA
                     VRQALAYLKTLDQDGDGIPENTNFPDQTYDTWVMQGISAYCGSLWLAALEAGAAMADL
                     QGAPDTAAQYRAWFAQAQPRFESALWNGRYYNYDTGAHGYHDSVMADQLAGQWYADAT
                     GLPPIVPVTHTRSALDTVYQLNVRGFGDGQMGAVNGMRPDGAPDTSNMQAEEVWTGTT
                     YALAGFMLHQNMDDAAWATAYGV"
BASE COUNT          344 a          800 c          689 g          283 t
ORIGIN      
        1 gcaacggcga cgacggcccc tggcagggcg cgccggtggg cggcctcggc gcgggcagca
       61 ttgggcgcac ctatcgcggc gacttcgcgc gctggcacct ggcccccggc gagaaccgct
      121 acggcaccgt ctgggccgac cagttcagcg tgcgcgtggc ccgccccgag gccccggtca
      181 gcgcccgcgt gctgttgccc gctcgcccac cccaggggct ggacgcctgg aactgggatt
      241 acccggcgga cggcggcacc tacgctgcgc tctatccctt tgcctggtac gactatggcg
      301 ccgctgccgc cggggtcacg ctgcaagccc gccagttctc gcccgtcatc ccgcacaact
      361 accaggagag cagctatccg gtcgccgtct tcgagtggac cgtgaccaac gacgccgcga
      421 cccccgccga tgtcagcctg ctattcacct gggagaaccc gctgggctgg gatgatcggg
      481 tggcgcgccc ggcgggcaac accaaccgca tccgccgcga cacgctcgac ggcgcgccca
      541 tgctcggcat cgtcatgggc cgcagcgccc ccgatggcac ccccggcccc acagccgtca
      601 agcaagcgtg ggacgggcag ttcgttatcg ccgcgcgcga gacgccgggc gtgcaactca
      661 gcgccctggg ccgcttcgcc acgcagggcg acggcgccga ggtctggcag ccgtgggccg
      721 cgacaggccg gctgccggat agcgacgatc ccgcccccgc ccgccagggc gaaccgattg
      781 gcgcggcgct ggcggcgagc acccacctgg cgcccggcga gagccgcacc atcaccttcg
      841 tgctggcctg ggacctgccg ctgatgcagt tcggcgcggg cgccaaagcc gagcaatggt
      901 accgccgcta caccgagtgg ttcggcaagg acggccacca cgcctgggag atcgcgcgca
      961 ccggcttgca gcaggcgccc gcctgggccg ccgcgctcac cgcctggcag gcgcccatcc
     1021 tggccggcac gcagcgcccg acgtggtata agacggcgct gttcaacgaa ctctattacc
     1081 tggtcgacgg cggcaccatc tgggaaaacg gccgcgtggg cgaccccacg cccagcacgc
     1141 accgcttctc ctacctggag tgctacgact atcccttcta ctccaccctc gacgtgcgct
     1201 tttacggctc cttcgcccag ttgatgctct ggccggagat cgagaaggcc gagatgcgcg
     1261 agttcgctct caccatcccg cagtccgacc cgacgcgtta tatcaccacc gatgtcgagc
     1321 accggcgcga ggccgtgcgc aaggtgccgg gcgcggcgcc ccacgacgtg ggcatgccca
     1381 gcgaagcgcc gtggacgcgc atcaacgcct acaactatca ggatgtgagc aactggaagg
     1441 acctcaacag caagttcgtg ctgctggtgt accgcgacta tatcgccacg ggcgaccggg
     1501 ccttcctgga cgccacctgg cccgccgtgc gccaggccct ggcctacctg aagacgctcg
     1561 accaggacgg cgacggcatc cccgagaaca ccaacttccc cgaccagacc tacgacacct
     1621 gggtgatgca gggcatcagc gcctattgcg gcagcctctg gctggcggcg ctcgaagccg
     1681 gggccgccat ggccgatctc cagggcgccc cagacaccgc ggcgcagtac cgcgcgtggt
     1741 tcgcccaggc gcagccgcgc ttcgagtcgg cgctgtggaa cggccgctac tacaactacg
     1801 acaccggcgc ccacggctac cacgacagcg taatggccga ccagcttgcc gggcagtggt
     1861 atgccgacgc caccggcctg ccgcccatcg tgccggtcac gcacacgcgc agcgcgctcg
     1921 acaccgtcta ccagcttaac gtgcgcggct tcggcgacgg ccagatgggc gcggtcaacg
     1981 gcatgcgccc cgacggcgcg cccgacacca gcaacatgca ggccgaagag gtctggaccg
     2041 gcacgaccta tgcgctggcc ggattcatgc tacatcagaa tatggacgac gcggcctggg
     2101 ccacggccta cggggt
//
LOCUS       DASUYC010000141         2988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1681376, whole genome shotgun sequence.
ACCESSION   DASUYC010000141 DASUYC010000000
VERSION     DASUYC010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1681376"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..1314
                     /locus_tag="VF276_02285"
     CDS             94..1314
                     /locus_tag="VF276_02285"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HEX6605720.1"
                     /translation="MQLRAGNEVMRGAVRGGDRAAPTLRVPVDAGRPGPQTTYHTRTW
                     LLWFLAAGLPALLTRNPLYLLLVMLAARGVDAAVAGYESAATPDAVDTRRQSWGGLLW
                     LGVGLAAVSALFNGLGAHFGTTVLIRLPDDWPIIGGPLTVEGFAYGALGGMSIVAVLT
                     VAAAFNTGADTYALLRSIPAAFSQAGLVTAIGLAYVPQTITRIREIHEAQLLRGHRFR
                     GARSVLPMAVPLLAGGLERAIQLAEAMESRGFSHPGPARPAPTGRAMGEPDRLTGALL
                     AIGAVAVALGAFALLYYRTAPLGGALVLAGGLGAIGWALRRVGQASRRTRYRREIWRR
                     RDTLVALTLGGALAVTLWSWATHSAWWGYTPYPTLHWPSFEPALGLALALLVAPALPL
                     LRREARPLEEGSSC"
     gene            1308..2975
                     /locus_tag="VF276_02290"
     CDS             1308..2975
                     /locus_tag="VF276_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019067162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6605721.1"
                     /translation="MLITVDHLTYTYPHAAQPAIQDVSLDVDEGEFLLLAGESGAGKS
                     TLLRALNGLVPHFYGGRIGGRVRVAGHDPVAEGPAPMSAVVGLVFQDPEAQVVADTVE
                     DELAFAMENAGLPQATMRKRIEEVLDQLSIAHLRGRRLSTLSGGERQRVAIGAVLTLQ
                     PRILVLDEPTSQLDPQAAEEVLDTLVKLNQDLGLTIILSEHRLERVVQHVDRIVFLGR
                     RPEAGPTPVLSGPPGEVLAAMPLAPPLAEVARALGWRPVPLSIKEARRRLSGDRRFTP
                     PPSDGENEAAVTPATDGSALETAGVSLRAENVWYTYGGREALRGLSLEARPGEILALM
                     GRNGAGKTTLLKLLVGLLKPGQGRVLVGGTDTRRLPLETIVRQVGYVPQRPDVLLFAD
                     NVRDELAFTRRNHGLPLDGAAVDDLVQRLHLTDYMDRDPKDLSVGERQRVALAAVLAA
                     EPGALLLDEPTRGMDYRQKDALAAVLRALAAAGRTIVLATHDVELAAHLADRVALISE
                     GQVVVHGPARRVMADSLVFASQVSKLFRDPRLVTAADVRARLAEIAV"
BASE COUNT          371 a         1062 c         1130 g          425 t
ORIGIN      
        1 cggccatccc ggccgggctg ctgctggccg ggctggtgct ggtgctgagc gggcggcgcc
       61 tggccgcgcg gcgcgcggag gactgagaga cagatgcaac tgcgggccgg gaatgaggtg
      121 atgcgcggag ccgtgcgcgg cggggaccgg gcggccccta ccctgcgcgt gcccgtggac
      181 gccgggcgcc ccggtccgca aaccacctat cacacgcgca cctggttgct gtggttcctg
      241 gccgcggggc tgcccgcgct gctgacccgc aacccgctct acctgctgct ggtcatgctg
      301 gcggcgcgcg gcgtggatgc cgccgtcgcc ggctatgaat ccgccgccac gccggacgcc
      361 gtggatacgc ggcggcaaag ctggggcggc ctgttgtggc tcggtgtggg gctggccgcc
      421 gtgtcggccc tgttcaacgg gctgggtgcc cacttcggca ccaccgtgct gatccgcctg
      481 cccgacgact ggccgatcat cggcgggccg ctgacggtgg agggcttcgc ctacggcgcg
      541 ctcggcggca tgtccatcgt ggccgtgctg accgtggcgg cggcgttcaa caccggcgcc
      601 gatacctatg ccctgctgcg gtcgatcccc gccgccttct cgcaggccgg gctggtcacg
      661 gccatcgggc tggcctatgt gccgcagacc atcacccgca tccgcgagat ccacgaggcg
      721 cagttgctgc gcggccaccg cttccgcggt gcgcgctcgg tcctgccgat ggccgtgccg
      781 ctgctggcgg gcgggctgga acgggccatc caactggctg aagcgatgga gtcacgcggc
      841 ttctcacacc ccggcccggc gcgtcccgcg cccaccgggc gcgccatggg cgaaccggat
      901 cggctcaccg gcgcgctgct ggcgattggc gcggtcgccg tggcgctggg ggcattcgct
      961 ctgctctatt accgtaccgc cccgctgggt ggcgcgctcg tgctggccgg ggggctgggc
     1021 gccatcggct gggcgctgcg ccgggtgggt caggcgagcc ggcgcactcg ctaccgccgc
     1081 gagatctggc ggcggcgcga tacgctggtc gcactgacac tgggcggcgc gctcgccgtc
     1141 acgctgtgga gttgggccac gcacagcgcg tggtggggct acacgcctta ccccacactg
     1201 cactggccgt cgttcgagcc ggcgctgggg ctggcgctgg ccctgctggt ggccccggcc
     1261 ctgccgttgc tgcgccggga ggcccggccc ctggaagaag gttcatcgtg ctgattaccg
     1321 tcgaccatct aacctatacc tatccgcacg cggcccagcc cgccattcag gatgtcagcc
     1381 tggacgtgga cgaaggcgag ttcctgctgc tggcgggcga gtcgggcgcg ggcaaatcga
     1441 cgctgctgcg ggcgctgaac gggctggtgc cgcacttcta cggcgggcgc atcggcgggc
     1501 gggtgcgcgt ggccgggcat gacccggtgg ccgaggggcc ggcgcccatg agcgctgtcg
     1561 tcggcctcgt cttccaggac cccgaagccc aggtggtggc cgacacggtg gaggacgaac
     1621 tggccttcgc catggagaac gccgggctgc cgcaggcgac catgcgcaag cgcatcgagg
     1681 aggtgctcga tcagctttcc atcgcccacc tgcgcgggcg gcggctcagc acgctgtcgg
     1741 gcggcgaacg gcagcgcgtg gcgatcggcg cggtgctcac gctgcaaccg cgcatcctcg
     1801 tgctcgacga accgacctcg caactcgacc cgcaagccgc cgaggaagta ctggacacgc
     1861 tggtcaagct gaaccaggac ctggggctga ccatcatcct gtccgagcac cggctggagc
     1921 gcgtggtgca gcatgtggac cgcatcgtct tcctggggcg gcgcccggag gccggcccca
     1981 cgccggtctt gagcgggccg ccgggcgagg tgctcgcggc gatgccgctg gccccgccgc
     2041 tggccgaggt ggcgcgcgcc ctgggctggc gcccggtgcc gctcagcatc aaggaggccc
     2101 gccgccggct cagcggcgac cggcgcttca cgccgccccc cagtgacggg gagaacgagg
     2161 cggcagtcac accggccact gacggcagcg cgctggaaac ggcgggagtc tcgctgcggg
     2221 ccgaaaacgt ctggtacacc tacggcgggc gcgaggcgct gcgcgggctg agcctggagg
     2281 cgcgccccgg cgagatcctg gccctgatgg ggcgcaacgg cgcgggcaag acgaccctgc
     2341 tcaagctgct ggtcggcctg ctcaagccgg gccagggccg cgtgctagtc ggcggcacgg
     2401 atacccgccg cctgccgctg gagaccatcg tccgccaggt gggctacgtg ccgcagcggc
     2461 ccgacgtgct gctgttcgcc gacaacgtgc gcgacgaact ggccttcacc cggcgcaacc
     2521 acggcctgcc tctggacggc gccgcggtgg acgatctggt gcagcggctg cacctgacgg
     2581 actacatgga ccgcgaccca aaggacctga gcgtgggcga gcgccagcgg gtggccctgg
     2641 ccgccgtcct ggccgccgag ccgggcgccc tgctgctgga cgagccgacc cgcggcatgg
     2701 actaccgcca gaaggacgcg ctggccgccg tgctgcgcgc cctggccgcc gccgggcgca
     2761 cgattgtcct ggcgacgcac gacgtggaac tggccgccca cctggccgac cgcgtggcgc
     2821 tgatcagcga ggggcaggtg gtggtgcacg gcccggcccg ccgggtgatg gccgattcgc
     2881 tggtcttcgc ctcgcaggtc agcaagctgt tccgcgaccc gcggctggtc acggcggcgg
     2941 acgtgcgcgc ccggttggcg gagatagcgg tatgagccgg gcgatcgt
//
LOCUS       DASUYC010000142         2047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_612273,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000142 DASUYC010000000
VERSION     DASUYC010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612273"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="VF276_02295"
     CDS             complement(<1..911)
                     /locus_tag="VF276_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605722.1"
                     /translation="MRKLFSLVGIFATLAFAITANVSAVFAAGPDAGGNPLAYTRFQV
                     TKDQAAADQPAQPGPLAGSGNLIYHTGGRVQTGTHHTYAIYWGSSFSTNYKTLVNRYF
                     GDVAADSGKTSNVYYSDTQYYQTISGVTTHISYSEQFKGSWSDTVLPSSSGCSSTAGG
                     TTKCVSDSQIRAEVTKAINANGWPTGLGNEYFVLLGNGISTCLGSSCAFTQFCAYHSH
                     YTLNSATVLYANQPYTGHNLSACGGGNYPNGDAAADSVLSVVSHEANETITDALGNAW
                     YDAAGYENGDKCAWNFGTQLGGSTGTH"
     gene            complement(1091..>2047)
                     /locus_tag="VF276_02300"
     CDS             complement(1091..>2047)
                     /locus_tag="VF276_02300"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX6605723.1"
                     /translation="AVATATGMPPVASPAPPAPPPAGPPSARRVADWPVVPVIDAAMQ
                     ARLRRILGLGLKMGNRPAVFAKAGDSITAAPAFLQAFACPGAGASVPADLAPVLAYFG
                     ATRFPADATKGGCGVADSFSRASVSAMPGWTSANALAPLRHPPPGCDPPYDTGLICEL
                     HLLRPGIVLIMFGTNDLGKSDDVDGFAGRLTVLVQQALALGTIPVLSTIPPRLDETEY
                     AKRVPLFNAAIVQVAAAQQVPLWNYWLALQTPGLPNHGLSVDGIHPSVYGCPACDPTN
                     LTPAGLRYGYNLRNWTALQVLATLKRVVIDAGSPDAGAGIDS"
BASE COUNT          309 a          640 c          753 g          345 t
ORIGIN      
        1 tagtgcgtgc cggtgctgcc gcccaactgg gtgccgaagt tccaggcgca tttatcgccg
       61 ttctcgtagc ccgcggcgtc gtaccaggcg ttgcctagcg catcggtgat cgtctcgttg
      121 gcctcatgcg ataccacgct gagcaccgag tcggcggcgg cgtcgccgtt ggggtagttg
      181 ccgccgccgc aggcgctcag gttatggccg gtgtacggtt gattggcgta gagcaccgtg
      241 gcgctgttga gcgtgtagtg cgagtggtag gcgcagaact gggtgaaggc gcaactgctg
      301 ccgaggcagg tgctgatgcc gttgcccagc agcacgaagt attcgttgcc cagccccgtc
      361 ggccagccat tagcgttgat cgccttggtc acttccgcgc ggatctgcga atcgctgacg
      421 cacttggtgg tcccgccggc agtggacgag cagccactgc tgctgggcag caccgtgtcg
      481 ctccacgacc ccttgaactg ttcgctgtag ctgatgtggg tggtcacgcc gctgatcgtc
      541 tggtagtact gcgtgtcgct gtaataaacg ttggaggtct tcccgctgtc ggcggccacg
      601 tcgccaaaat agcggttgac gagcgtcttg tagttcgtgc tgaaactgct gccccagtag
      661 atcgcgtagg tatggtgcgt gccggtctgc acccggccgc cggtgtggta gatcaggttg
      721 cccgacccgg ccagcgggcc gggttgcgcc ggctgatcgg cggcggcctg gtccttggtg
      781 acctggaagc gggtgtaggc cagcggattg ccgccggcgt ccggcccggc ggcgaagacg
      841 gcggacacgt tggcggtaat ggcgaacgcg agtgtagcga agatgcccac gagcgagaac
      901 agtttacgca tgatggtacc cttccgtcgc gagaggctgg aagtcgctac ccctgaaggc
      961 cgcggggggc gggtgccccg ccggtacatg gccgtgtacc ctggcgcacc tgggcgctat
     1021 tttagcacaa tttcaactcc gcaaggggaa ttagtccaat tatatatgcc gcaataacac
     1081 cactagttag tcaggaatcg attccagcgc ccgcatcggg cgaaccggca tcgatgacca
     1141 cgcgcttgag ggtggcgagc acttgcagcg cggtccagtt gcgcaggttg tagccgtaac
     1201 gcaggccggc gggggtcagg ttcgtggggt cgcaggccgg gcagccgtag acgctggggt
     1261 ggatgccgtc gacgctcagg ccgtggttgg gcaggccggg cgtttgcagc gccagccagt
     1321 agttccagag cggtacctgc tgcgcggcgg ccacctgcac gatggcggcg ttgaacagcg
     1381 gcacgcgctt ggcgtactcg gtctcgtcga gccggggcgg gatggtgctc agcaccggga
     1441 tggtgcccag cgccagcgcc tgctgcacca gcaccgtgag ccgccccgcg aagccgtcca
     1501 cgtcgtccga cttgcccagg tcgttggtgc cgaacatgat cagcacgatg ccggggcgca
     1561 gcaggtggag ctcgcagatc agccccgtat cgtagggcgg atcgcagccg ggcggcgggt
     1621 ggcgcagggg cgccagggcg ttggcgctgg tccagcccgg catcgcgctc acgctggccc
     1681 ggctgaacga gtcggcgacg ccgcagcccc ccttcgtcgc atccgccggg aaccgggtcg
     1741 cgccaaaata ggcgagcacc ggcgccaggt ccgccggtac cgacgcacca gcgccggggc
     1801 aggcgaacgc ttgcaggaac gcgggcgccg ccgtgatcga gtcaccggcc ttggcgaaga
     1861 cggcgggccg gttgcccatc ttgaggccga gccccaggat gcggcgcagc cgggcttgca
     1921 tggccgcgtc gatgaccggc acgaccggcc aatcggccac ccggcgggcg gatggcggcc
     1981 cggcgggcgg gggcgcgggg ggcgcggggc tggcaaccgg cggcatcccc gtcgccgtgg
     2041 cgaccgc
//
LOCUS       DASUYC010000143         3349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1094567, whole genome shotgun sequence.
ACCESSION   DASUYC010000143 DASUYC010000000
VERSION     DASUYC010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094567"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="VF276_02305"
     CDS             <1..950
                     /locus_tag="VF276_02305"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX6605724.1"
                     /translation="GDQGSPATLGSTLSDRPSYPEGDHYGLFDALDTTTGKLAWQMKV
                     PERAVSGITVAGDLVFLGESNGKFDAVDAKSGTVLWSYKPTEPNAGGANGASAVYVMN
                     GREYVVMAFGGNTQVRSGQNSPPGDVLIAFALPQAGQGAPNVVTAQPTQIPSGAIPDS
                     AMVPPATSAPPDATVVEVHAHNIHFYPDHLTATPGQKLALHLMNVEPGEVPHNFFIAL
                     PAGIIGTKGVLEPGQDGYLVITAPEQPGDYFFWCDVGSHRFQGMNGTLTVAAAGGGAP
                     GMPRTGGGGLAWDSLLISLIAVLLLTAGVALLGRRRVSR"
     gene            1279..>3349
                     /locus_tag="VF276_02310"
     CDS             1279..>3349
                     /locus_tag="VF276_02310"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX6605725.1"
                     /translation="MRNTGHPLRTSAWARGGALALLLLSLLAGPGVAGYAAPPPARAA
                     VAGTAADWVPGRVLVKPRAGIAMAALAPVLGRLGLAASGPATAGVLAVQGGADLDVAA
                     TVRALRADPAVAYAEPDYRVHTLLVPNDERYADEEWWLTAIQAQAAWDLSTGDPNLIV
                     AILDTGVATDHPEFAGRILPGHNFVANNNDAYDDNGHGTHVAGIASAMGNNGKGIAGI
                     SWQSKILPVKVLNGEGQGSTYDFTQGIRWAVDSGARVINISAGADFSTETEHDAVKYA
                     HDKGVVIVAAAGNTPDGKPEYPAGYDEVIAVSASSRRDSVAGFSSYGDYVDVAAPGIN
                     ILSTWVDHRKPGYQSESGTSMASPVVAGAAALILARNPALNPEQVQLLLQQTADDIGD
                     PGFDPKSGWGRLNLLHALQQAPAGPRPGSTLPPTRTPPGASGSHIQGTVAGVDPAFVD
                     VLLLPQNGAPNRDIQPDAAGLYVFDNLSAGKYTVQLALKTGGFNFPAPQVVELNGRTD
                     HTAQVNFSLVARRPAAFENVPAPDPAPDLVYFPPVQHTLRGPFLAYWNAHGGLPIFGY
                     PISEEFQEVSATDGKPYTVQYFQRNRFEYHPEFAGTPNAVLLGLLGVERSKDRNFPPG
                     TPGVTDATHQWFPPVQHNLSGSFLQYWNTHGGLAIFGYPISEEFPEVSATDGKTYTVQ
                     YFQRNRFE"
BASE COUNT          533 a         1235 c         1125 g          456 t
ORIGIN      
        1 acggcgacca gggcagcccg gccaccctcg gctcgacgct gagcgaccgg cccagctacc
       61 cggaaggcga ccactatggc ctgttcgacg cgctggacac gacgacgggc aagctggcct
      121 ggcagatgaa ggtgcccgaa cgcgccgtct ccggcatcac cgttgcgggc gacctggtgt
      181 tcctgggcga gagcaacggc aagttcgacg ccgtggacgc caagagcggc acggtgctct
      241 ggtcctataa gcccaccgag cccaacgcgg gcggggcgaa cggcgcatcg gcggtgtatg
      301 tcatgaacgg gcgcgagtac gtcgtcatgg ccttcggcgg caacacgcag gtacgctccg
      361 ggcagaactc gccgccgggc gacgtgctga tcgcgttcgc gctgccgcag gccggccagg
      421 gcgcgccgaa cgtggtcacc gcccagccga cgcaaatccc cagcggggcc atcccggact
      481 cggcgatggt gccgcccgcc accagcgcgc cgcccgacgc cacggtggtc gaggtgcatg
      541 cccataacat ccacttctac ccggatcacc tgacggcgac tcctgggcag aagctggcgc
      601 tgcatctcat gaacgtcgag ccgggggagg tgcctcacaa cttcttcatc gccctgccgg
      661 cgggcatcat cggcacgaag ggtgtcctgg aacccggcca ggacggctac ctggtcatca
      721 cggcgccgga acagccgggc gactatttct tctggtgtga cgtcggctcg caccgcttcc
      781 agggcatgaa cgggacgttg acggtggcgg ccgccggtgg cggtgcgccc ggcatgcccc
      841 gcaccggcgg gggcggtctg gcctgggaca gcctgctgat cagcctgatc gcggtgctgc
      901 tcctgacggc gggcgtcgcc ctgctgggcc ggcggcgggt gtcccgctag acgcaaggtg
      961 cccgctcggc ggcgggcggg ggccggttcg gaagcgcacc ggtgcccccc gccgccgagc
     1021 acggccagtc ccggcctcgt gccgcggcat ccccatggcg ccgcgacacg gggcgggcga
     1081 ccgccgccgt ttcgggccaa acggcccgtc gataagcgcc gatattcccc cagtttacgc
     1141 cgcctatagg cagacttgac tgcctctggt ataataaacg cggttcctct ccatcccgcc
     1201 gggaccgggc gccggattgc tgccgccatg ccgggcaggc acctcgttaa ctctcacacg
     1261 ctgttagaag gacgatccat gcgaaacacc gggcatccgc tacgaacttc cgcctgggcg
     1321 cgcgggggcg cgctcgcttt gttactgctg agcctgctgg ccgggccggg cgtggccggg
     1381 tatgccgcgc caccgcccgc gcgggccgcc gtcgccggga ccgccgccga ctgggtgccg
     1441 ggccgcgtgc tggtgaagcc gcgcgccggg atcgcgatgg cggcgctggc gcccgtgcta
     1501 ggccgcctgg ggctggccgc gtccggcccg gcaacggcgg gcgtgctggc cgtgcagggc
     1561 ggggccgatc tcgacgtggc ggcgaccgtg cgcgccctgc gcgccgaccc cgccgtcgcc
     1621 tatgccgaac ccgactaccg cgtccacacc ctgctcgtgc ccaacgacga gcgctacgcc
     1681 gacgaagagt ggtggctgac ggccatccag gcccaggcgg cctgggacct cagcaccggc
     1741 gaccccaacc tgatcgtggc catcctcgac accggcgtgg cgaccgacca tcccgagttc
     1801 gcgggccgca tcttgcccgg ccacaacttc gtcgccaaca ataacgacgc ctacgacgac
     1861 aacggccacg gcacccacgt cgcgggcatc gccagcgcga tgggcaacaa cggcaagggc
     1921 atcgccggca tctcctggca gagcaagatc ctgccggtga aggtgctcaa cggcgagggc
     1981 caggggtcca cctacgactt cacccagggc atccgctggg ccgtggacag cggcgcgcgg
     2041 gtgatcaaca tcagcgccgg ggccgacttc tcgactgaga ccgagcacga cgccgtgaaa
     2101 tacgcccacg acaagggcgt ggtgatcgtg gccgcggcgg gcaacacgcc cgacggcaag
     2161 cccgaatacc cggcaggcta cgacgaggtg atcgccgtca gcgccagcag ccgccgcgac
     2221 agcgtggccg gcttctcgtc ctacggcgac tatgtggacg tggccgcgcc cggcatcaac
     2281 atcctgagca cctgggtgga ccaccgcaag cccggctacc agagcgagag cggcacgtcg
     2341 atggcgagcc cggtggtggc cggggcggcg gcgctgatcc tggcccgcaa cccggcgctg
     2401 aatcctgagc aggtgcaact cctgctgcaa cagacggcgg acgacatcgg cgaccccggc
     2461 ttcgacccga agtcgggctg ggggcggctc aacctgctgc acgcgctgca acaggcgccc
     2521 gcgggaccgc gacccggctc gaccctgccg cccacgcgca ccccgcccgg cgcatccggc
     2581 tcgcacatcc agggcacggt cgcgggcgtg gacccggcgt tcgtcgacgt gctgctgctg
     2641 ccgcagaacg gcgcgcccaa ccgcgacatc cagcccgatg ccgccgggct gtacgtgttc
     2701 gacaatctgt ccgcaggcaa gtacaccgtg caactggcgc tcaagaccgg cggcttcaac
     2761 ttccccgcgc cgcaggtggt cgagctcaac gggcgcaccg accacaccgc gcaggtgaac
     2821 ttcagcctgg tggcccggcg ccccgccgcc ttcgagaacg tgcccgcccc ggaccccgcg
     2881 cccgacctgg tctacttccc gcccgtgcaa cacaccctgc gcgggccgtt cctggcctat
     2941 tggaacgccc acggcggcct gcccatcttc ggctatccca tctcggagga gttccaggag
     3001 gtgagcgcga ccgacggcaa gccttacacc gtgcaatatt tccagcgcaa ccgcttcgaa
     3061 tatcacccgg agttcgcggg cacgccgaac gccgtgctgc tgggcctgct cggcgtcgaa
     3121 cgcagcaagg accgcaactt cccgcccggc acgcccggtg tgaccgatgc cacgcaccag
     3181 tggttcccgc ccgtgcagca caacctgagc ggttcgttcc tgcagtattg gaacacgcac
     3241 ggcggcctgg ccatcttcgg ctacccgatc agtgaggagt tcccggaggt cagcgccacc
     3301 gacggcaaga cctacacggt gcaatatttc cagcgcaacc gcttcgagt
//
LOCUS       DASUYC010000144         3333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1680898, whole genome shotgun sequence.
ACCESSION   DASUYC010000144 DASUYC010000000
VERSION     DASUYC010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1680898"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1188)
                     /locus_tag="VF276_02315"
     CDS             complement(<1..1188)
                     /locus_tag="VF276_02315"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEX6605726.1"
                     /translation="MLQHHPSQRTLRRGLTLLAGAVLVTLLMPTGWSAPAFAALPAQA
                     PGDNTQYFPATSHQVSGALLDFYRRAGGTDRFGAPLTEQYQDGAQVYQIFERGALQYN
                     PQSGAAAVRALGQELVAGRAVDAPVSVGTNGPDGLFVMETGHTVGRAFAPYWQKAGTW
                     DRFGKPISEQLVEGDRAVQYFEFGKLTADPAAPNGIREQLLDSVRAAGSPFAPAAGAT
                     DATALFVAATGHSIAGGFREVYQALGADASFGAPLTDEVLENGLMVQYFSAGKLSWSG
                     ARPAGHQVQVEAVGKDWMATHSVPAAALQPVAAPVATPAPDAQGGVKVPAGWTLVAQG
                     QTTYKGSSEARTKNIVLAAKKLDGVTVPAGGVFSFNRSLGDDTEKAGFVVALIIYNGR
                     TIEG"
     gene            complement(1317..2810)
                     /gene="gatA"
                     /locus_tag="VF276_02320"
     CDS             complement(1317..2810)
                     /gene="gatA"
                     /locus_tag="VF276_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEX6605727.1"
                     /translation="MNTTDLSYLALHEVRAMLDRGEMSSLELAEACLARMAAVDGAVH
                     AFLTITDEVAREQARASDTRRANGVVLSPLDGIPMSLKDILVTAGIRTTAGSRMLENF
                     VPPYNGTVVQRLLDAGAVLLGKANQDEFAMGSSTENSYFGPTRNPWDLSRVPGGSSGG
                     SAAAVAAGMGYFSLGTDTGGSIRQPAALCGVVGVKPTYGRVSRSGVVAFASSLDQIGP
                     FARTARDAALVLGVIAGQDPLDATTGTEPVDDYAGALTADLRGLRVGVPREYGVDRLP
                     AEVRDPVQAAIAQFESLGATIQEVSLPHSEYALAAYYIIAPAEASANLARYDGVKYGF
                     RAETTGTDADSAGVDLMSRTRGAGFGAEVKRRIMLGTYGLSSGYYDAYYKKAQQVRTL
                     VAQDFANAFTQVDLLLGPTSPTPAFRVGEVDDPLQMYLMDIYTIPANLAGICGVSVPC
                     GFAGRLPIGLQILARPFAEATLLRAAHAYEQSTTWHLRHPDLPVAAT"
     gene            complement(2815..3108)
                     /gene="gatC"
                     /locus_tag="VF276_02325"
     CDS             complement(2815..3108)
                     /gene="gatC"
                     /locus_tag="VF276_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX6605728.1"
                     /translation="MLTTEEVAHVARLARLDLAPDELETMRRQLSSVLDHINRLNEVD
                     TSASPPTAQVFALRDVVRLDSPRPPFPQAVMLANAPDAADGCFKVPAVLETEG"
BASE COUNT          495 a         1143 c         1186 g          509 t
ORIGIN      
        1 gccctcgatc gtccgcccgt tatagatgat taacgccacc acgaagccgg ccttttcggt
       61 gtcgtcaccg aggctgcggt taaaggagaa cacgccgccc gcgggcacgg tcacgccgtc
      121 gagtttcttg gcggccagca caatgttttt cgtgcgcgcc tcgctcgacc ccttgtacgt
      181 cgtctgcccc tgggcaacca gcgtccagcc ggcgggcacc ttcacgccgc cctgcgcgtc
      241 gggggcaggg gtcgccaccg gcgcggcgac gggctgcaag gcggcggcgg gcacgctatg
      301 cgtcgccatc cagtccttgc ccacggcttc gacctgcacc tggtgcccgg cgggccgcgc
      361 gcccgaccag gagagcttgc ccgcgctgaa gtactggacc atcaggccgt tctccagcac
      421 ctcgtcggtc agcggcgcgc cgaacgaggc gtccgccccc agcgcctggt acacttcgcg
      481 gaacccgccg gcgatgctgt ggccggtcgc cgcgacaaac agcgcggtgg cgtccgtggc
      541 acccgcggcg ggcgcgaacg gcgacccggc ggcgcgtacg ctgtccagca gttgctcacg
      601 gatgccgttg ggcgccgcgg ggtccgccgt cagcttaccg aactcgaagt actgcacggc
      661 gcggtcgcct tcgaccaact gctcgctgat cggcttgccg aagcggtccc aggtgccggc
      721 cttctgccag taaggcgcga acgcgcggcc cacggtgtgc ccggtttcca tgacgaacag
      781 accgtccggg ccgttggtgc ccaccgagac gggcgcatcg acggcgcgcc cggccaccag
      841 ttcctggccc agcgcgcgca cggccgcggc gccgctctgc ggattgtatt gcagggcgcc
      901 gcgctcgaag atctggtaga cctgcgcgcc atcctggtat tgctcggtca gcggcgcgcc
      961 gaagcgatcc gtgccgcccg cgcggcggta aaagtcgagc agcgcgccgc tgacctggtg
     1021 cgacgtggcc gggaagtatt gcgtgttatc gccgggcgcc tgcgcgggca gcgcggcgaa
     1081 ggccggagcc gaccatcctg tgggcatgag cagcgtcacc agtaccgcgc ccgccagcag
     1141 ggtcaggccg cggcggagag tacgctggga tgggtggtgc tgaagcatga ataaaaccgt
     1201 ccttctctcc gagtacggat aaattgggtg gtgtaccggc gcgtcaaagg tgcgtcagct
     1261 atggcgccgt ccggtacgtg cgatagatag agtgtctgcg cccctatcga attgcgctag
     1321 gttgcggcca ccgggaggtc cgggtggcgc aggtgccagg tggtcgactg ttcgtaggcg
     1381 tgcgccgcgc gcaagagcgt tgcctccgcg aacggccgcg ccaggatctg caggccgatc
     1441 ggcagccggc ccgcgaagcc acagggcacc gacaccccac agatgccggc cagattcgcg
     1501 gggattgtgt aaatatccat caggtacatc tgcaacggat catccacctc gcccacgcgg
     1561 aaggcgggcg tcggcgatgt cgggccaagc agcagatcca cctgcgtgaa agcgttggcg
     1621 aagtcctggg ccaccagcgt gcgtacctgc tgggccttct tgtagtacgc gtcgtagtag
     1681 cccgaactca gaccataggt gccgagcatg atgcggcgct tgacttcggc gccgaagccc
     1741 gcgccgcgcg tccgcgacat caggtccacc ccggcgctgt cggcatcggt gccggtggtc
     1801 tcggcccgga acccgtactt caccccgtca tagcgggcca ggttggcgct cgcctcggcg
     1861 ggggcgatga tatagtaggc ggccagcgca tattcggagt gcggcaggct cacctcctgg
     1921 atcgtcgcgc ccagcgactc gaactgggcg atggccgcct gcaccgggtc gcgtacctcg
     1981 gcgggcaggc gatccacgcc gtactcgcgg ggcacgccga cgcgcaggcc gcgcaaatcg
     2041 gcagtcaacg cacccgcgta atcgtccacc ggctcggttc cggtggtcgc gtccagcggg
     2101 tcctgccccg cgatgacgcc cagcaccagg gccgcgtcgc gggcggtgcg ggcgaacggc
     2161 ccgatctggt cgagcgaact ggcgaacgcg accacgcccg agcgcgacac ccgcccatag
     2221 gtcggcttga cgccgacgac gccgcagagc gcggcgggct ggcggatgct gccgccggta
     2281 tcggtgccca ggctgaagta gcccataccg gcggccaccg ccgccgccga gccgccgctg
     2341 ctgccgcccg gcacccggct gagatcccag gggttgcgcg tggggccgaa atacgagttc
     2401 tcggtgctcg accccatggc gaattcgtcc tggttggcct tgcccagcaa gacggcgccc
     2461 gcatcgagca ggcgctgcac caccgtgccg ttgtagggcg gcacgaagtt ctccagcatg
     2521 cgcgacccgg cggtggtgcg gatgcccgcc gtcaccagga tgtccttgag gctcatcggg
     2581 atgccgtcga gcgggctgag caccacgccg ttggcacggc gggtatcgga ggcgcgcgcc
     2641 tgttctcggg ccacttcgtc ggtgatggtc aggaaggcgt gaacggcgcc gtccaccgcc
     2701 gccatgcgcg ccaggcaggc ctcggccagt tccaggctgg acatctcgcc ccggtcgagc
     2761 atggcgcgca cctcgtgcag cgccagatag ctgagatcgg tggtgttcat cggcttagcc
     2821 ctcggtctcc aggacggcgg gcaccttgaa gcagccgtcg gcggcatcgg gggcgttcgc
     2881 cagcatcacc gcctgcggga acggggggcg cgggctgtcc aagcggacta cgtcgcgcag
     2941 ggcgaatacc tgcgccgtgg gcgggctggc cgacgtgtcc acttcgttca agcggttgat
     3001 atggtcgagc accgacgaga gctggcggcg catcgtctcc agttcgtcgg gcgccaggtc
     3061 gagccgggcc agacgagcca catgggccac ttcttcggta gtcagcataa atcagcgatt
     3121 ccttccggtc ggggccgccg ctcacgcagc ggcgcggcag cgggcggtgg gcttacacgc
     3181 gcacgccgca ctgcgcgcaa aaggcggcgt gcgggcgcag cggggcgccg cagttttggc
     3241 aatattcggt ctggccgggc gcgggcacac ggcgggtgcc ctggtcctcc aggttcggca
     3301 gcgggcgcgc cgggtagttg gacgccggcg cgg
//
LOCUS       DASUYC010000145         3205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_614881,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000145 DASUYC010000000
VERSION     DASUYC010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614881"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..1511
                     /locus_tag="VF276_02330"
     CDS             222..1511
                     /locus_tag="VF276_02330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6605729.1"
                     /translation="MRVLMLSWEWPPNMVGGLGKHVVELLPELVDEDVEVHLVTPRLK
                     GDVHDDAIISAATGKPAENGSHFYRVDPFPYQVGNFYANTAQTNVTLEAACQRIIDQI
                     GGFDLIHAHDWLVSFAAITLKHTRRLPLISTIHATEMGRNHGFLWEDMQRDIHTAEWR
                     LVYESWRTIACSNYMAWEIMNYFGAPAEKVDVIPNGVDPRPLDRWNGQDLSWFRNTLA
                     RPDEKIVFYVGRLVWEKGLQVLVDAAPQIVAIYPNVKFIIAGGGDFRFDLANRARAAG
                     VGDWFFFPGRISDDDRDRLYKVADCAVFPSLYEPFGIVALEAMAAGCPVVATDTGGLS
                     EVINLHENGLKVYPNDPGSLAWGILQTLVHPDWARARAVNARRDVDTLYNWDRIAKMT
                     KAVYERVAGEARAGDWAYHTQPVQQGAEPVEPVTPLV"
     gene            1530..2144
                     /locus_tag="VF276_02335"
     CDS             1530..2144
                     /locus_tag="VF276_02335"
                     /inference="COORDINATES: protein motif:HMM:NF012895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605730.1"
                     /translation="MLLSDKRILEEMDKGNVIIEPFERRQLGTNSYDVRLGQWYFLPN
                     HNMVTVSFFDAEQSRNFWGEPQRAHDGMIAIRPGDTILAHTEEIIGGQNGFTTSMRAR
                     SSIGRSCLSVCKCAGVGDVGYISHWTMEITNHSHAMVLLPVGLRVAQILFYEVGETLG
                     EYKGKYGVRDGWTPQDMLPKLYRDWDLDRLGKARMQLVGEDGWR"
     gene            2135..2569
                     /locus_tag="VF276_02340"
     CDS             2135..2569
                     /locus_tag="VF276_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605731.1"
                     /translation="MALRCEAGLVYCREVADGLCSICERPFCARHGNLAGPYCQRCKR
                     AFVERQRAVEAAAAEATRREMAAQHNSDGQCGWIACTGPLLALCQHCGLQYCSQHSNR
                     YRYSYRYQTRRGVERRHATIILCDACKPALKEYKREKTWLEV"
     gene            2619..>3205
                     /locus_tag="VF276_02345"
     CDS             2619..>3205
                     /locus_tag="VF276_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605732.1"
                     /translation="MTERRQTRVTMTPRLQARLTVLVERLHLTGLPVERVNEVAAALD
                     EVITQIGAAEHGVHGREWVVALRTAQNVAPLLRDTCDLARSLGPAPDEPQMHRHPVQT
                     TQALWIWLITQAELYCKLQLVHMGLALRDPGDEDDDDDTEMFDFTGHLGEELVRQIGL
                     SEGVVLDVWRRDRYRLYRILFSNYTRLRTYTAARG"
BASE COUNT          600 a         1072 c         1017 g          516 t
ORIGIN      
        1 ggcttcggct tccgccagac caccgaagcg tatgatattt acggtcattg gctcgacgaa
       61 tagctattag ccgtcagccg tcagctatca ggtttaacga taaaagccgc tccgtccagc
      121 gtagaactca ggtgcaggcc aggaagcggc aaagccggga ttagagtgct gatagctgac
      181 ggctgatagc taatagctga cggcttcgca ggaggggtta catgcgcgtt ctgatgctct
      241 cgtgggagtg gcccccgaac atggtgggcg gcctcggcaa gcacgtcgtg gaactgttgc
      301 ccgaactggt ggatgaagac gtggaggtcc acctggtcac gccgcgcctc aagggcgatg
      361 tgcatgacga cgccattatc tcggcggcga ccggcaagcc cgccgaaaac ggcagccact
      421 tctaccgcgt ggatcccttc ccctaccagg tgggcaactt ctacgccaac accgcgcaaa
      481 ccaacgtcac cctcgaagcg gcctgccagc gcatcatcga ccagatcggc ggcttcgacc
      541 tgatccacgc gcacgactgg ctggtgtcct tcgccgccat cacgctcaaa cacacgcgcc
      601 gcctgcccct gatctcgacc atccacgcca cggagatggg ccgcaaccac ggctttctgt
      661 gggaagacat gcagcgcgac atccacaccg ccgagtggcg tctggtctac gaaagctggc
      721 gcaccatcgc ctgcagcaat tacatggcct gggagatcat gaactacttc ggcgcgcctg
      781 ccgagaaagt cgacgtgatc cccaacggcg tcgatccgcg cccgttagac cgctggaacg
      841 ggcaggacct gagctggttc cgcaacaccc tggcccgccc cgacgaaaag atcgtcttct
      901 acgtgggccg cctggtctgg gaaaaaggct tgcaggtgct cgtcgacgcg gcgccgcaga
      961 tcgtggcgat ctaccccaac gtcaagttca ttatcgcggg cggcggcgac ttccgcttcg
     1021 acctggcgaa ccgggcgcgc gccgcgggcg tgggcgattg gttcttcttc cccggccgca
     1081 tttcggacga cgaccgcgac cggctctaca aggtcgccga ttgcgccgtc ttccccagcc
     1141 tctacgagcc gttcggcatc gtcgcgttgg aagcgatggc ggcgggctgc ccggtggtcg
     1201 ccacggacac cggcgggctg tcggaggtga tcaacctgca cgagaacggc ctgaaagtct
     1261 acccgaacga tcccggctcg ttggcctggg gtattctgca aacgctggtg catcccgatt
     1321 gggccagggc gcgcgcggtg aacgcccgcc gcgatgtcga taccctctac aactgggacc
     1381 gcatcgccaa gatgaccaag gccgtctacg agcgggtggc cggcgaggcc cgcgccggcg
     1441 attgggccta ccacacccag cccgtccagc agggagccga gccggtggag ccggtcacac
     1501 cgctggttta actagtcagg agacaacctg tgctgctatc cgacaagcgt atcctggaag
     1561 agatggacaa aggcaacgtc attatcgagc cgttcgagcg ccgccagctt ggcaccaact
     1621 cgtatgatgt gcgcctgggc cagtggtact ttctgcccaa ccacaacatg gtcaccgtca
     1681 gcttcttcga cgccgagcaa tcccgcaatt tctggggcga gccgcaacgt gcccatgacg
     1741 gcatgatcgc catccgcccc ggcgacacca tcctggccca caccgaggag atcatcggcg
     1801 ggcagaacgg cttcaccacg tcgatgcggg cgcgcagcag catcgggcgc agttgcctgt
     1861 ccgtctgcaa atgcgcgggc gttggcgacg tgggctacat ctcgcactgg accatggaga
     1921 tcaccaacca cagccacgcc atggtgctgc tgccggtggg cctgcgcgta gcgcagatcc
     1981 tcttctacga ggtgggcgaa accctgggcg agtacaaagg caaatacggc gtgcgcgacg
     2041 gctggacgcc gcaggatatg ctgcccaagc tctaccgcga ttgggacctg gaccggctgg
     2101 gcaaggcacg gatgcagcta gtaggcgagg acggatggcg ttaaggtgcg aagccgggct
     2161 ggtgtattgc cgggaagtgg ccgacggcct gtgcagcatc tgcgagcgcc cgttttgcgc
     2221 gcgccacggc aacctggcag gcccctactg ccagcgctgt aagcgcgcct tcgtcgagcg
     2281 gcaacgcgcc gtcgaggccg ccgccgccga ggccacgcgg cgcgagatgg cggcccagca
     2341 taacagcgac ggccagtgcg ggtggatcgc ctgcaccggg ccgttgctgg cgctgtgcca
     2401 gcattgtggc ctgcaatact gctcgcagca cagcaaccgc taccgctata gctatcgcta
     2461 ccagacgcgg cgcggcgtcg aacggcggca cgccacgatc atactctgcg atgcctgcaa
     2521 accggccctc aaggaataca aacgagagaa aacctggctc gaagtgtgat cccgccccgg
     2581 caagcgcctg gggcgtgtcg cgatccgaga tctgatatat gaccgagcga cgccagaccc
     2641 gcgtcaccat gaccccgcgc ctgcaggccc gcctgaccgt gctggtcgag cggctgcatc
     2701 tcaccgggct gccggtggag cgcgtgaacg aggtcgcggc agccctcgac gaggtcatta
     2761 cccagatcgg ggcggcggag cacggcgtcc acgggcgcga gtgggtggtc gccctgcgca
     2821 cggcgcagaa cgtggcgccg ctgctgcgcg acacctgcga cctcgcgcgc agcctcggcc
     2881 ccgcccccga cgaacctcag atgcaccggc acccggtgca aaccacgcaa gccctctgga
     2941 tctggttgat cacgcaggcc gagctctact gcaaactcca gcttgtccat atgggcctgg
     3001 ccctgcgcga ccccggcgac gaggacgacg acgacgacac cgagatgttc gatttcaccg
     3061 gccacctggg cgaggaactg gtccgccaga tcgggctatc cgagggcgtg gtgctggacg
     3121 tgtggcggcg cgaccgctac cggctgtacc gcatcctctt cagcaactat acccggctcc
     3181 gtacctatac cgccgcgcgc ggctg
//
LOCUS       DASUYC010000146         2559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1778529, whole genome shotgun sequence.
ACCESSION   DASUYC010000146 DASUYC010000000
VERSION     DASUYC010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1778529"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="VF276_02350"
     CDS             <1..562
                     /locus_tag="VF276_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6605733.1"
                     /translation="LGLGIAQPLYTLIVQNAFPPQRLGVVTAATTFFRSIGSTVGVAV
                     FGTVVNNRFSSEYAARLPDQLKASPQFGAFLSNLTPQALISPDTIKAVQAQLQSLGLP
                     AGQVQQTIAAIEAPIRPALGVATTSAFLIGAVVLALSVVLVAFIPEIALRRRVSRQAA
                     SVETTAVEGTTEAFSPEPAGVAARRD"
     gene            846..2105
                     /locus_tag="VF276_02355"
     CDS             846..2105
                     /locus_tag="VF276_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012520341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEX6605734.1"
                     /translation="METFTASYRFNPSTHRFAFATGIENSYPVITGRDGRPFRVDEMA
                     KAHHYERWREDFALVRELGLTYLRYGPPYYQTHRGPGRYDWEFADATLGELRRLGITP
                     IVDLCHFGVPDWVGNFQNPDWPALFAEYARAFAERYPWVRLYTPVNEIYICAKFSASF
                     GWWNEQLRSDHAFVTAIKHLVRANILAEEAILEVQPGAYFIQSESSEHFHPISHDPAV
                     QELAEHMNHARFVSLDLCYGHHDMCWCVYQWLLDHGMTRDEFAWFQRHGRALKPYHIM
                     GNDYYVTNEHMVHPDGRLSPAGDAFGYYMVTKQYYERYRLPVMHTETNMADAEQAPLW
                     LWKQWTNMLRLKQDGVPVVGFTWYSLTDQVDWDTALREDNGRVNPLGLFDLDRQIRPV
                     GEAYRRLVTGWSEVLPRESIFHAVAAA"
     gene            complement(2305..>2559)
                     /locus_tag="VF276_02360"
     CDS             complement(2305..>2559)
                     /locus_tag="VF276_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605735.1"
                     /translation="RQAVLRAATDDVPDDLSRLAATVDRLFCDFVEAQMARHPDLARE
                     LCRRCSNVMARRRAVVAATQMDLVLRRFLPAPPLPPPPPV"
BASE COUNT          404 a          867 c          891 g          397 t
ORIGIN      
        1 cctgggcctg ggcatcgccc agccgctcta cacgctgatc gtgcagaatg ccttcccgcc
       61 gcagcggctg ggcgtggtca cggcggcgac gaccttcttc cgctccatcg gcagcacggt
      121 cggcgtggcg gtgttcggca ccgtcgtcaa caaccgcttc agcagcgagt acgcggcccg
      181 cctgccggac cagctcaagg ccagcccgca gttcggggcc ttcctgagca acctgacgcc
      241 gcaggccctg atcagcccgg acacgatcaa ggcggtgcaa gcgcagttgc aaagcctggg
      301 cttgccggcg gggcaggtcc agcaaacgat cgccgcgatt gaggcgccga tccggccggc
      361 gctgggggtg gcgacgacga gcgcgttcct catcggcgcg gtggtgctgg ccctgtccgt
      421 ggtgctggtg gccttcatac ccgaaattgc cctgcggcgc cgcgtgagcc gccaggcggc
      481 cagtgtggag accaccgccg tggagggcac gaccgaggct ttcagcccgg agccggcggg
      541 ggtagccgcg cgccgggact agcggtcgct ccgggccgcc ggggacttcc ggcgccgcgc
      601 ccccgcgcgg cgccgggacc aacgtcccta aagccgggga cgcccggtta caatccggta
      661 atttatcttg acgcgcgatc ccaaccgcgt tatgctgagg gtaatcgtcc gcgccctggt
      721 tcgcccccgt tgtgcctcag agccggcgct gcccgcggac gttgggacac tgccttcgac
      781 cgacaatggt gtcgagagaa aggggatcgc ggggcctgca ccgggcccat gccgccgcgg
      841 agactatgga aaccttcacc gcttcttacc gttttaaccc cagcacgcac cgcttcgctt
      901 tcgccaccgg catcgagaac agctaccccg tcatcaccgg gcgggatggc cggcctttcc
      961 gcgtggacga aatggcaaag gcgcatcatt acgagcgctg gcgcgaggac ttcgccctgg
     1021 tgcgcgagct gggcctgacc tatctgcgct acggaccgcc ctattaccag acgcatcgcg
     1081 ggccggggcg ctacgactgg gagttcgccg acgcgacgct gggcgagttg cgccggctgg
     1141 gcatcacgcc catcgtggac ctgtgccatt tcggcgtgcc cgactgggtg ggcaatttcc
     1201 agaaccctga ctggccggcg ctgttcgccg agtatgcccg cgccttcgcg gaacgctatc
     1261 cgtgggtgcg gctctacacg cccgtcaacg agatctacat ctgcgccaag ttctcggctt
     1321 ccttcggctg gtggaacgag cagttgcgca gcgatcatgc cttcgtgacg gcgatcaagc
     1381 acctggtgcg ggccaatatc ctggccgagg aagcgatcct ggaggttcaa ccgggcgctt
     1441 acttcatcca gagcgagtcg agcgagcact tccaccccat cagccacgac ccggcggtgc
     1501 aggaactggc cgagcacatg aaccacgcgc gcttcgtgtc gctggacctc tgctacgggc
     1561 accacgacat gtgctggtgt gtctaccagt ggctgctcga ccacggcatg acccgcgacg
     1621 agttcgcctg gttccagcgc cacgggcgcg cgctcaagcc ctaccacatc atgggcaacg
     1681 actactacgt caccaacgag cacatggtcc accccgacgg ccggctctcg cccgcgggcg
     1741 acgccttcgg ctactatatg gtgaccaagc agtattacga gcgctaccgc ctgccggtga
     1801 tgcacactga gacgaacatg gccgacgccg agcaggcgcc gctctggctg tggaagcagt
     1861 ggaccaacat gctgcgcctc aagcaagacg gggtgccggt ggtgggcttc acgtggtaca
     1921 gcctgacaga ccaggtggac tgggacacgg cgctacgcga ggacaacggg cgcgtcaacc
     1981 cgctgggcct gttcgacctg gaccgccaga tccgcccggt gggcgaggcc tatcggcggc
     2041 tggtgacggg ctggagcgag gtgctgcccc gcgagagcat cttccacgcg gtggccgccg
     2101 cgtaggcccg gccccggcgc gcataccgac agggcacgtc cggctggcgg gcgtgccttt
     2161 gtttgtgccc gccccgaccc tcaagggtcg ggctacgtca acgcagcccg cctccgcggg
     2221 ctgggggaga gggacaggcc gcgcaggcgg ccttcgtaag gctagccgga ggcttgagcc
     2281 tccgggcgtt gcctccgggc gggctcagac gggcggaggt ggcggcagag gcggggcagg
     2341 caggaagcgg cgcagcacca ggtccatctg tgtggcggcg accacggcgc ggcggcgggc
     2401 catgacgttg ctgcaccgcc ggcagagttc gcgggccagg tcgggatggc gggccatttg
     2461 cgcttcgaca aagtcgcaga agaggcggtc cacggtggcg gcgaggcggc tcaggtcgtc
     2521 gggcacgtcg tcggtggcgg cgcgcaacac ggcctggcg
//
LOCUS       DASUYC010000147         2141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_524127,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000147 DASUYC010000000
VERSION     DASUYC010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524127"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            619..1392
                     /locus_tag="VF276_02365"
     CDS             619..1392
                     /locus_tag="VF276_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6605736.1"
                     /translation="MNRGSHNGAPAGRAGSPYAVEATDLTRVLPLGAEQVPVLNGISL
                     QIAPGEWVALTGPSGSGKSTLLGILAGLDTPSGGRICIDGADITGMDERDLARIRNQK
                     IGIVFQSFNLIPTLTAQENVEVPLYVRANHRQAPARAREMLERVGLGHRRGHRPHQLS
                     GGEQQRVAIARALVTEPALLLADEPTGNLDSTTGAQVLDLFARLRADLGVTLIMATHD
                     PAIAARAGRELHIVDGRLTDPPARPAEPAADLAVAGGPR"
     gene            1389..>2141
                     /locus_tag="VF276_02370"
     CDS             1389..>2141
                     /locus_tag="VF276_02370"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6605737.1"
                     /translation="MTARFYLRYAARALQRGGQRTVLALLCVAFGVMSLVAMQLLAGL
                     IREAMMGDPRLVTGGDGLISVRETGALTAADLATLDRLRADGTISNYSLLARQDMFML
                     KPGNGNPVYLLTHPPRGVDPATFPLVGSIPLRAPAGLTFAAAIAPPLHAVITRDLADG
                     LGLKPGDHFKLIGSPDGSAPELTVAGVAESMPDSQGDAVLYSLDTARELAHTALVADA
                     ATVTWPGGGAVPARLEASGLPVIPAAQQAERMG"
BASE COUNT          322 a          838 c          697 g          284 t
ORIGIN      
        1 gtcctcgatc ggttgcgcca ccagcaaccc cgccatccgc ttcgcatcgc gttccgcccc
       61 ctgcaacgcc gggagatccg tgcggggcgt gccctcataa ttgcagccga tgaccactcc
      121 aaacagccga ttcatatgca tcctcctggc gggcgcgacc ggaggctcaa gcctccggct
      181 accgttacca aggccgcctg cgcggcctcc cgtctcagcc cgcgcaggcg ggcttcgcaa
      241 atgtagcccg cccgttgacg ggccgggcgc tccccctgcc gcccgttgac gggccgggcg
      301 ctcccaccct cccgcctgcc ggactcaccc ccttaaggca atggcgtaaa ccggcgcagg
      361 ttactgcgaa gacaggacga tatatctccc gcgaatcttc acaatttcag cacacccctg
      421 cgctacagta aggatagcgg ctcgcggcgc cgccgggccg cggcgtgccg taacacacgg
      481 taacacaacc accggcgcgc gggtaacatt cgggtgctac gataagccca acacggcgcc
      541 gcccgtatcc ccggcaggcc aggccacgcc tgcgcggcac gggtggttcg cgccaattcc
      601 ctggacaagg agtagtgaat gaatcgagga agccataacg gtgcgccggc ggggcgcgcc
      661 ggaagcccgt acgccgtcga ggcgaccgac ctcacgcggg tgcttccgct cggcgccgag
      721 caggtgcccg tcctcaacgg catctcgttg cagatcgccc ccggcgagtg ggtggcgctg
      781 accgggccgt ccggctcggg caaatcgacc ctgctgggca tcctggccgg gctggacacg
      841 cccagcggcg ggcgcatctg catcgacggc gcggacatca ccggcatgga cgagcgcgac
      901 ctggcccgta tccgcaacca gaagatcggc atcgtcttcc agtcgttcaa cctcatcccc
      961 acgctgacgg cgcaggagaa cgtcgaggtg ccgctctatg tgcgcgccaa ccaccgccag
     1021 gcgccggcgc gggcgcgcga gatgctggag cgcgtgggcc tgggccaccg ccgcgggcac
     1081 cggccccacc agttatcggg cggcgagcaa cagcgcgtcg ccatcgcccg cgccctggtg
     1141 acggagccgg ccctgctgct ggccgacgag cccaccggca acctcgacag caccaccggc
     1201 gcgcaggtgc tcgacctgtt cgcccgcctg cgcgccgacc tgggcgtgac cctgatcatg
     1261 gcgacccacg acccggccat cgccgcccgc gccggtcgcg aactccacat cgtggacggc
     1321 aggctcaccg acccgcccgc ccggcccgcc gagccggccg cggacctggc cgtcgcggga
     1381 ggcccccgat gacggcgcgc ttctacctgc gctacgcggc gcgtgccctc cagcgcggcg
     1441 gtcagcggac ggtgctggcc ctgctctgcg tggccttcgg cgtcatgtcg ctggtcgcca
     1501 tgcaactgct ggccgggctg atccgcgaag ccatgatggg cgacccgcgc ctggtgacag
     1561 gcggcgacgg cctgatctcc gtgcgcgaga ccggcgcgct caccgccgcc gatctcgcga
     1621 cgctcgaccg cctgcgagca gacggcacga tcagcaacta cagcctgttg gcccgccagg
     1681 atatgttcat gctcaagccg ggcaacggca acccggtcta cctgctcacc cacccgccgc
     1741 gcggcgtgga cccggccacc ttcccgctgg tgggcagcat ccccctgcgc gcacccgccg
     1801 ggctgacctt cgcggcggcc atcgccccgc cgctccacgc tgtgatcacg cgcgacctgg
     1861 ccgatgggct gggcctgaag ccgggcgacc acttcaagct catcggcagc cccgacggct
     1921 cggcgcccga actgaccgtg gccggcgtgg ccgagagcat gcccgactcg caaggcgacg
     1981 ccgtgctcta cagcctggac acggcccgcg aactcgcgca caccgcgctg gtggccgacg
     2041 cggccaccgt gacctggccg ggcggtggcg cggtcccggc ccgcctagaa gcgagcggcc
     2101 tgcccgtcat ccctgccgcc cagcaggccg agcgcatggg t
//
LOCUS       DASUYC010000148         6033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1447947, whole genome shotgun sequence.
ACCESSION   DASUYC010000148 DASUYC010000000
VERSION     DASUYC010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6033
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447947"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1629
                     /locus_tag="VF276_02375"
     CDS             <1..1629
                     /locus_tag="VF276_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-binding domain-containing protein"
                     /protein_id="HEX6605738.1"
                     /translation="TLIDENGAPAGRKVFALYNPPLVHRELGLRRSAMLETTDLAGQF
                     LANDIQTIVFARSRTAVEILLTYLRTAMAQHRRDPEKVRGYRGGYLPLQRREIERGLR
                     DGKVRAVVSTNALELGIDIGTLEVCLMAGYPGTIAGTWQQAGRAGRRSGTALAMFVAG
                     SSPLDQFLVQHPDYFFARSPEHGLINPDNLLVLMQHLQCAAFELPFAEDEAYGDHPSA
                     ETALMLGYLAEGGVLYHSGEGWHWSSENFPAENVSLRTAAADNFVIIDTTDPTPRVIG
                     EMDRFSVPTLLHEEAIYLHEGTQYHVDKLDWDEQKGYVRQVEVDYYTDANLAVNLKVL
                     DVLSGEDRHAPRSHGEVMVSALATIYKKIKLGTHENIGWGRIHLPEQEMHTSAYWLTV
                     PPQVVADLPKGALQAALVGLAHVLSYLGPLYLMCDPHDLGVVPQVKSPFTEAPTIFVY
                     DSYAGGIGFSKTLYEQHATLLRAAHALVAACGCDEGCPSCIGTAAELGEQGKQQTLTL
                     LRGLLAVAAHLPPPASKEAPPPPEDRWEVAAGVD"
     gene            1768..2649
                     /locus_tag="VF276_02380"
     CDS             1768..2649
                     /locus_tag="VF276_02380"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX6605739.1"
                     /translation="MDFLLWLQGLRNPVLTAFFGAWTFLGSEEFLLVFVPLIYWCVSR
                     VAGLRIALVFFGSQYLNEVLKDLANEPRPGPPVVQLYPDTALGGGWPSGHAQNTGAVW
                     GTLAGLVRRAWVWGLAVVVTLLVGFSRLYLGLHWPIDVVSGWVIGAAVVGVALAVIGA
                     QARRASPPIPLPWLAGALVVPVLLLAVHPTNTTAKIAGALLGTFLGWWLERRLVGFEA
                     FGSMGQRAIMAAIGLVVAFGLRTVLKPVFGVLPGDILPDLLRYSIILLWVVWLAPAVF
                     VRLFGRAAAPTAVAAKG"
     gene            complement(2905..4476)
                     /gene="aceB"
                     /locus_tag="VF276_02385"
     CDS             complement(2905..4476)
                     /gene="aceB"
                     /locus_tag="VF276_02385"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018466514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HEX6605740.1"
                     /translation="MESDIQLTGSAVPGSDEILTPGALDFVARLHREFNPTRQDLLAR
                     RAERQTRLDAGELPDFLPETASIRGGDWVVAATPPDLQKRRVEITGPAERKMMINALN
                     SGASVFMADFEDALSPTWDNIVGGQINLRDAIRRTIEFSNPDGKVYRLNEQTATLLVR
                     PRGWHLPEKHVLVDGEPISASLFDFGLYFYHNAQELLARGSGPYFYLPKLESHLEARL
                     WNSVFNAAQDALGIPRGTIRATVLIETILAAFEMEEILFELREHSAGLNAGRWDYMFS
                     AIKKFRNRENLILPDRAQITMTVPFMRAYTELLVKSCHTHGAHAIGGMAAFIPNRRDP
                     EVTERALAKVREDKEREAGDGFDGTWVAHPDLVPVAMAEFDRVVGDQPHQKARRREEV
                     QVQASQLLDLRVPGGKITEAGARNNISVALQYLNAWLQGNGAVAIFNLMEDAATAEIS
                     RAQLWQWLRHGAQLDDGRPFTPDLYKQFRQEERAKLGGTAEERYSDAVAILDSLALAP
                     QFTEFLTLPAYQELP"
     gene            complement(4645..5958)
                     /gene="glcF"
                     /locus_tag="VF276_02390"
     CDS             complement(4645..5958)
                     /gene="glcF"
                     /locus_tag="VF276_02390"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014690046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HEX6605741.1"
                     /translation="MSIFDQHHPPPADLLDDCVHCGFCLPTCPTYVLWGEEMDSPRGR
                     IYLMNLGADGQAEMNDTYVRHFDQCLGCMACVTACPSGVQYDKLIEAVRPQIERHYPR
                     SLPDRLFRQMLFQLFPYPNRLRALAFPLWFYQASGVRALVHRSGMLDRLPARLQAMES
                     LLPRVTPRQLRRRFPERIRARGPARRRVGLLLGCVQRVYFSDVNAATARVLAAEGCEV
                     VIPTTQGCCGALLVHAGQEDAALDFARRTIDAFEAADVDTIAINAAGCGSTMKEYGWL
                     LRDDPAYAERAKAFSAKCRDIMEILADLEPRAPRHPLPLRVAYHDACHLQHAQGVRAQ
                     PRAVLATIPGLEVVEIPESALCCGSAGIYNMVEPEPAMQLGDRKVANVLKTGADVLAS
                     ANPGCLLQIRNGLQRAGRDLPVVHTVELLDAAIRGVRPAALAQHD"
BASE COUNT          855 a         1922 c         2260 g          996 t
ORIGIN      
        1 acgctgatcg acgagaacgg cgcgcccgcc gggcgcaaag tcttcgcgct ctacaacccg
       61 ccgctggtcc accgggagtt gggcctgcgc cgctcggcca tgctggagac gaccgacctg
      121 gccgggcagt tcctcgccaa cgacatccag accatcgtct tcgcccgctc gcgcacggcg
      181 gtggagatcc tgttgaccta cctgcgcacg gcgatggccc agcaccggcg cgacccggag
      241 aaggtgcgcg gctaccgggg cggctacctg ccgttgcaac ggcgcgagat cgagcgcggg
      301 ctgcgcgacg gcaaggtgcg cgccgtggtc agcacgaacg cgctggaact gggcatcgac
      361 atcggtacgc tggaggtctg cctgatggcg ggctatcccg gcaccatcgc cggcacctgg
      421 cagcaggccg ggcgcgccgg gcggcgcagc ggcacggccc tggcgatgtt cgtggcggga
      481 agcagtccgc tcgaccagtt cctggtgcag caccccgact acttcttcgc ccgttcgccg
      541 gagcacggcc tgatcaaccc ggataacctg ctggtgctga tgcagcatct acaatgcgcc
      601 gccttcgagc tacccttcgc cgaggacgag gcctacggcg accacccgtc cgccgagacg
      661 gcgctgatgc tgggctacct ggccgagggc ggggtgctgt accatagcgg cgagggctgg
      721 cactggagca gcgagaactt ccccgccgag aacgtgtcgc tgcgcaccgc cgccgccgac
      781 aacttcgtca tcatcgacac gaccgacccg accccgcgtg tcatcggcga gatggaccgc
      841 ttcagcgtgc cgacgctgct gcacgaggag gcgatctacc tgcacgaggg cacgcaatac
      901 cacgtcgata agctggactg ggacgagcag aagggctatg tgcgccaggt ggaggtggac
      961 tactacaccg acgccaacct ggcggtgaac ctcaaggtgc tggacgtgct gtcgggcgag
     1021 gaccgccacg cgccgcgctc gcacggcgag gtcatggtca gcgcgctggc gacgatctac
     1081 aagaagatca agctgggcac gcacgagaac atcggctggg ggcgcatcca cctgcccgaa
     1141 caggagatgc acacctcggc ctactggctg accgtgccgc cgcaagtggt ggccgacctg
     1201 ccgaagggcg cgttgcaggc cgcgctcgtc ggcctggccc atgtgctgtc ctacctgggg
     1261 ccgctctacc tgatgtgcga cccgcacgac ctgggcgtgg tgccgcaggt gaagtcgccc
     1321 ttcaccgagg cgccgaccat cttcgtctac gacagctacg cgggcggcat cggcttcagc
     1381 aagacgctct acgagcagca cgccacgctg ctacgcgccg cccacgccct ggtggccgcc
     1441 tgcggctgcg acgagggctg cccctcctgc atcggcacgg cggcggaact gggtgagcag
     1501 ggcaagcagc agacgctgac cctgctgcgc ggcctgctgg ccgtcgccgc ccacctgccc
     1561 ccgcctgcct ctaaagaggc accgccgccc cccgaagacc gctgggaagt cgccgcgggc
     1621 gtggattagc cggtgcctgg cacagattcc ctgacagaca ctgttatggt atagtagagc
     1681 cgcctcgggt gtgtcctgaa gcggctctcg ttttgttccg tagctcataa gagcatgtaa
     1741 ttgcgggagg gaggcccggt cgcgttcatg gactttctgt tgtggttgca gggcttgcgg
     1801 aacccggtcc tgacggcgtt tttcggcgcg tggacctttc tcggatcgga agagttcttg
     1861 ctggtgtttg tgccgctgat ctactggtgc gtgagccggg tggccgggct gcgcatcgcg
     1921 ctggtcttct tcgggtcgca gtatctgaac gaggtgctga aggacctggc gaacgagccg
     1981 cgaccggggc cgccggtggt gcaactctac ccggacacgg cgctgggcgg cggctggccg
     2041 agcgggcacg cccagaacac cggggcggtg tggggcacgc tggccgggct ggtgcgccgg
     2101 gcctgggtct gggggctggc ggtggtcgtc accctgctgg tcggcttctc gcggctctac
     2161 ctgggcctcc actggcccat cgacgtggtc agcggctggg tcatcggcgc ggcggtggta
     2221 ggggtggcgc tggcggtgat cggggcgcag gcgcggcgcg cgtcgccgcc catcccgctg
     2281 ccctggctgg cgggcgcgct cgtcgtgccg gtgctgctgc tggccgtcca ccccaccaac
     2341 accacggcca agatcgccgg ggcgctgctg ggcaccttcc tgggctggtg gctggagcgg
     2401 cggctggttg gctttgaggc gttcggcagc atggggcagc gggcgatcat ggcggccatc
     2461 gggctggtgg tggcgttcgg gctgcgcacg gtgctcaagc cggtgttcgg cgtgctgccg
     2521 ggtgatatcc tgcccgatct gctgcgctac agcatcatct tgctctgggt ggtctggctg
     2581 gccccggcgg tgttcgtgcg gctgttcggg cgggctgctg cgcccacggc ggtggccgcg
     2641 aaggggtagc ggcgcgggga ggttctcccc cgatcccgaa cgagacgaga ttcttcgctg
     2701 cgctcagaat gacaggcgca tgatccccca cgagacgaga ttcttcgctg cgctcagaat
     2761 gacaagaaaa tgcctatcat tggttctttt gtcactctga gcgcagcgaa gagtctcgtc
     2821 ctggtttctc ccgcgagacg agattcttcg ctgcgctcag aatgacaggg gatggcgggc
     2881 gggcggctcg gcagggcggt ggcgctacgg caactcctgg taggcgggca gggtcaggaa
     2941 ctcggtgaac tgcggggcca gggccaggct gtcgaggatg gcgacggcgt cgctgtagcg
     3001 ctcttcggcg gtgccgccga gcttggcccg ctcttcctgg cggaactgct tgtagaggtc
     3061 gggcgtgaag gggcgcccgt cgtctagttg cgcgccgtgg cgcagccact gccagagctg
     3121 cgcgcgcgag atctcggcgg tggcggcgtc ttccatcagg ttgaagatgg cgacggcgcc
     3181 gttgccttgc agccaggcgt tgagatattg cagcgccacg ctgatgttgt tgcgcgcgcc
     3241 cgcctcggtg attttgccgc cgggcacgcg caggtccagc aactggctcg cctgcacctg
     3301 cacttcctcg cggcggcggg ccttctggtg cggctggtcg ccgaccacgc ggtcgaactc
     3361 ggccatcgcc accgggacga ggtcggggtg ggcgacccag gtgccgtcga agccgtcacc
     3421 cgcctcgcgc tctttgtcct cgcgcacctt cgccagcgcg cgctcggtga cttcggggtc
     3481 gcggcggttg gggatgaagg cggccatgcc gccgatggcg tgggcgccgt gggtgtggca
     3541 cgacttgacc agcaactcgg tgtaggcgcg catgaagggc acggtcatgg tgatctgggc
     3601 gcgatcgggc aggatcaggt tttcgcggtt gcggaacttc ttgatcgcac tgaacatgta
     3661 gtcccagcgc ccggcgttca gcccggcgga atgctcgcgc aactcgaaca ggatctcctc
     3721 catctcgaag gcggccagga tcgtctcaat aagcacggtg gcgcggatgg tgccgcgcgg
     3781 gatgccgagc gcgtcctggg cggcgttgaa gacgctgttc cagaggcggg cctccaggtg
     3841 gctttccagc ttgggcaggt agaagtacgg cccgctgccc cgagccagca gttcctgcgc
     3901 gttgtggtag aagtagagcc cgaagtcgaa caggctggcc gagatcggct cgccgtcgac
     3961 caggacgtgc ttctccggca ggtgccagcc gcgcgggcgc accagcaggg tggccgtctg
     4021 ctcgttgagg cgatagacct tgccgtcggg gttgctaaac tcgatcgtcc gcctgatggc
     4081 gtcgcgcagg ttgatttgcc cgccaacgat gttgtcccag gtaggcgata gggcgtcctc
     4141 gaagtcggcc atgaagacgc tggcgccgga gttgagcgcg ttgatcatca tcttgcgctc
     4201 ggcggggccg gtgatttcga cccggcgctt ctgcaggtcg gggggggtgg cggccaccac
     4261 ccagtcgccg ccgcggatgg acgccgtttc gggtaggaag tcgggcagtt cgcccgcgtc
     4321 gaggcgcgtt tgccgctcgg cccggcgggc gagcaggtcc tggcgggtgg ggttgaactc
     4381 gcggtgcagg cgggccacga agtcgagcgc gccgggggtc aggatctcgt cgctgccggg
     4441 gacggcggag ccggtgagtt gtatgtcgct ttccatagca tcaggttccc ttctgtactg
     4501 cagcgccggg gcgcccgatc ccagtgccgg gcgcctgcgg tcttgggtct gtggcctcac
     4561 cgggatggtc cagtggtgcg ggcagacggg gcgagcaacg gggcctgcgg gcggcgccgg
     4621 gggtagccgc gtcgcgggcc agggtcagtc atgttgggcc agggccgccg ggcgcacgcc
     4681 gcggatggcg gcgtcgagca actcgacggt gtggacgacg ggtaggtcgc ggcccgcgcg
     4741 ctgcaggccg ttgcggatct gcaaaaggca gccggggttg gcgctcgcca gcacgtcggc
     4801 gcccgtcttg agcacgttgg cgactttgcg gtcgccgagt tgcatggccg gttcgggttc
     4861 gaccatgttg tagatgcccg ccgagccgca gcagagcgcc gactcgggga tctcgaccac
     4921 ctccaggccg gggatggtcg ccaggacggc acgcggttgg gcgcggacgc cctgggcgtg
     4981 ttgcaggtgg caggcgtcat ggtaggcgac gcgtaggggc agcgggtggc gcggggcgcg
     5041 cggctccagg tcggccagga tctccatgat gtcgcggcac ttggcggaga aggctttggc
     5101 gcgctcggcg taggccgggt cgtcgcgcag cagccagccg tactccttca tggtggaacc
     5161 gcagcccgcc gcgttgatgg cgatggtgtc cacgtccgcc gcctcgaagg cgtcgatggt
     5221 gcgccgggcg aagtcgagcg cggcgtcctc ctgcccggcg tgaaccagca gcgcgccgca
     5281 gcagccttgc gtggtgggga tcaccacctc gcagccctcg gcggccagca cgcgggccgt
     5341 ggcggcgttt acgtcgctga agtagacgcg ctgcacgcag cccagcagca ggcccacgcg
     5401 ccgccgggcc gggccgcgcg cgcggatgcg ttccgggaac cggcggcgca actggcgcgg
     5461 cgtgacgcgc ggcagcagcg actccatggc ctggagccgg gcgggcaacc ggtcgagcat
     5521 gccggagcgg tggaccaggg cgcgtacccc ggaagcctgg tagaaccaga gcgggaaagc
     5581 cagcgcgcgc agccggttgg gataggggaa caactggaag agcatctgcc ggaacagccg
     5641 gtcgggcagg ctgcgcgggt agtggcgctc gatctgcggg cgcacggcct cgatcaactt
     5701 gtcgtactgc acgcccgacg ggcaggccgt gacgcaggcc atgcagccga ggcactggtc
     5761 gaagtggcgc acataggtgt cgttcatctc cgcctggccg tcggcgccca ggttcatcag
     5821 gtagatgcgg ccccgtggcg agtccatctc ctcgccccag aggacgtagg tggggcaggt
     5881 gggcaggcag aagccgcagt ggacacagtc atcgagcagg tcggcgggcg gcgggtggtg
     5941 ctggtcgaag atgctgatcg gcgcgccacg tccggcgggg ccgtggccgt tgggtgcggt
     6001 agggccgtcg ccgtggatcg ccggcgcgcc cga
//
LOCUS       DASUYC010000149         3063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_633550,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000149 DASUYC010000000
VERSION     DASUYC010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633550"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2820
                     /locus_tag="VF276_02395"
     CDS             <1..2820
                     /locus_tag="VF276_02395"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6605742.1"
                     /translation="YYFEQLHMWHIVPAAGRTLTLLDCCYSAAILGSPPPAARSAEPH
                     SAPEDQVRSAAAIAQEFDARAQATLAMQPGHLVIAACAANEQARELPRFGHGVFTYYV
                     LAHWRDHPGEVSYGSLVQYVDTMLEKAGLPGAAHGDAAHDRFVLRAGSAPGRAVLHVP
                     YPRNPRFVGQADMLAQLSTLLHTHAVVALTGVSGIGKTELACEFAYRNREQFPGGVFW
                     LAMDPPEGIAPQIAAYAGPAGLNLSGAGARSFDQRIAAVRAHWDHPLPSLLIFDNLAD
                     PAVLDHWRPRGPGARVLITSRRQPWAADRGIEMLALDGLTRSAGHSLLLTPRAHRHGA
                     SATTLPAPDGPLVDELCGELGDFPLALSLAAAYLEVHPDRTVGDYLAAIRGQALAHAS
                     LDDFSLALALATTYLQAHPGRTLGDYLAEVRTQVLAHDSFRATLEEPLPTEHEAGIVA
                     TFALSYNELLGTTPGDTLARTLLHRAAHAAPTPIPRALLAAVVDLDSVDPAANRSLDT
                     ALRCLAGLGLVDTLPDGSSLLHPLLAAYARSRAPDPGADAAALERALIAAAYQLLRAG
                     NLPAAQPYLPHLRATATAAMPRTDLPAATLLNNLASLLRAQGDYAAARPLYERALAIA
                     EQTLGPTHPDTAGSLTNLASLLQAQGDYAAARPLHERALAIAEQSLGPTHPDTATSLN
                     YLAGLLYGMGDYAAARPLYERALAIAEQTLGPAHPATVAGLDNLASLLSAQDDYAAAR
                     PLLERALAIREQTLGPTHLQTAQSLSNLASLLYGMGDYAAARPLYERVLAIRERALGP
                     THPDTATSLNYLASLLQAQGDPAAARALYERALAIAEQSLGPTHPTTAASLNNLAALL
                     RAQGDSGAARPLLERALTIAEQTLGPTHAATAISLNNLAELLQAEEDPGAARSLYARA
                     LAVAEQALGPSHPTTRAIRQNLHSL"
BASE COUNT          448 a         1258 c          893 g          464 t
ORIGIN      
        1 tactatttcg agcagctgca catgtggcac atcgtgccgg cggcgggccg caccctcacg
       61 ctcctcgatt gctgctacag cgccgccatc ctgggcagcc ccccgcctgc ggcgcgctct
      121 gcggaacctc acagcgcccc cgaggaccag gttcggtccg ccgctgcgat tgctcaagag
      181 ttcgatgccc gcgcccaggc gacgctcgcc atgcagccgg ggcatctcgt cattgccgcg
      241 tgcgcggcta acgagcaggc tcgcgaactg ccccggttcg gccacggtgt cttcacttac
      301 tatgtcctgg cccattggcg cgatcaccct ggcgaagtga gctacggtag cctggtccag
      361 tacgtcgaca ctatgctgga aaaggcgggc ctccccggtg cggctcatgg tgacgccgcc
      421 cacgaccgct ttgtgttgcg ggcaggatcg gcccctgggc gggccgtcct ccatgtgccc
      481 tatccgcgca atccgcgctt cgtgggccag gcggacatgc tcgcgcagct cagcaccctg
      541 ctccacacgc acgccgtggt cgcgctcacg ggcgtaagcg gcatcggcaa aaccgagctg
      601 gcttgcgagt ttgcgtaccg gaaccgggag caatttccgg ggggcgtttt ctggctggcc
      661 atggaccccc cggaaggcat cgcgccgcag atcgccgcct acgccggacc ggccggcctg
      721 aacttatcgg gtgcaggcgc gcgctccttt gatcagcgaa tcgccgcggt gcgcgcgcac
      781 tgggatcacc cgctccccag cctgttgatc ttcgacaacc tggccgaccc ggcggtcctg
      841 gaccactggc gcccgcgcgg accaggcgca cgcgtgctga tcaccagccg tcgccaaccc
      901 tgggccgccg accggggaat cgaaatgctc gccctcgatg gtctcacccg gtctgccggc
      961 cacagcctat tgctcacacc ccgcgcccac cggcatggcg cgagcgcgac cacgctcccc
     1021 gcgcccgacg gtcccctggt cgatgaactc tgtggcgaac tcggcgactt cccgctcgcg
     1081 ttgtccctgg ccgcggcgta cctggaagtc caccccgatc gcaccgtcgg cgactatcta
     1141 gccgcgatcc gcggccaggc gctggcccat gcttcgctcg acgacttttc cctggcgctg
     1201 gccctggcga cgacgtacct gcaagcccac cccggtcgca ccctcggcga ctacctggcc
     1261 gaggtccgca cccaggtgct ggcccatgat tcgttccggg ccacgctgga agagccattg
     1321 cccaccgaac atgaggccgg cattgtcgcc accttcgcct tgagctataa cgaactcctc
     1381 ggcaccacgc cgggcgatac cttggcccgc acgctcctgc atcgcgcagc gcacgcggcg
     1441 cccaccccga tcccccgtgc cctgcttgcg gctgtcgtcg atctcgactc tgtcgatccg
     1501 gccgcgaacc ggagtctgga caccgcactc cggtgcctcg ccggcctcgg cctggtcgat
     1561 acgcttcccg acggcagctc cctgctccat cccctcctcg cggcctacgc ccgttcccgc
     1621 gcccccgatc ccggcgccga tgccgccgcc ctggagcggg ctctgatcgc tgccgcctat
     1681 caactgttgc gcgccggcaa cctcccggct gcccaaccct acctgcccca cctgcgcgcc
     1741 accgccaccg cggccatgcc gcgcaccgac ctgccagcgg ccaccctgct caacaacctg
     1801 gcgagcttgc tgcgcgcgca aggtgactac gcggcggccc gccccctcta cgagcgggcc
     1861 ctggcgattg ccgagcagac cctcggcccc acgcaccccg acaccgccgg tagccttacc
     1921 aacctggcca gcctgctaca ggcccagggc gactacgccg ctgcgcgccc gcttcacgag
     1981 cgggccctcg ccattgccga gcagagcctc ggccccacgc accccgacac cgccaccagc
     2041 ctcaattacc tggccgggct actctacggt atgggcgact acgcggcggc ccgccccctc
     2101 tatgaacgcg ccctggccat tgccgagcaa accctcggcc ccgcccaccc tgccacggtc
     2161 gccggcctcg acaacttggc cagtctgctg tccgcccagg acgactacgc ggcggcccgc
     2221 cccctcctgg agcgcgccct cgccatccgc gagcagaccc tcggtcccac ccacctccag
     2281 acggcccaga gcctcagtaa cctggccagc ctgctgtatg gtatggggga ctacgcggcc
     2341 gcgcgcccgc tctacgagcg cgtcctggct atccgcgagc gcgccctcgg gcccacccat
     2401 cccgacaccg cgacgagcct caactacctg gcgagcctgc tacaggctca gggtgaccct
     2461 gccgccgccc gcgccctcta tgagcgcgcg ctggccattg ccgagcagag cctcggcccc
     2521 acccacccga ccacggccgc cagtctcaac aacctggcgg cattgctacg cgcccagggc
     2581 gactccggcg ccgcccgccc cctgttggag cgcgccctca cgattgccga gcagaccctc
     2641 ggccctaccc acgccgccac ggccatcagc ctcaacaacc tggccgaact gctgcaggct
     2701 gaggaggacc ctggcgccgc ccgctcgctc tacgcgcgag ccctggccgt ggccgagcaa
     2761 gccctcggcc ccagccaccc gaccacgcgc gcgattcggc agaacctgca cagcctctag
     2821 cctcccagga cgcgcctcca acttgccgcc ccgccgccgg tagcgcgccc gcgcccgctt
     2881 cttcccgacc aggcccgtcc gcgaccctgc tcgcgggcgc cggctgaccg ggaaatgagc
     2941 agtttttggt ctggaaaatg agcagcgaaa ccgcatacga tacgactgtc gaccgacagc
     3001 cgtatttgtt tgtggagggg gccgccatgt gtgaagcgat tcctccggta gtcaacgatc
     3061 tcc
//
LOCUS       DASUYC010000150         5010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1697064, whole genome shotgun sequence.
ACCESSION   DASUYC010000150 DASUYC010000000
VERSION     DASUYC010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1697064"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VF276_02400"
     CDS             complement(<1..465)
                     /locus_tag="VF276_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6605743.1"
                     /translation="MALQAEADGSAGAPEGARPAVDPARDFYDLDGLLTPQERDVRDR
                     VRAFVDAEVTPIINPYWERAEFPAHLIPKLAALGICGGVLQGYGCPGLSSVATGLASM
                     ELARGDASLCTFFGVTSGLAMSSIYYCGSEAQKQRWLPGLARMDLIGAFGLTE"
     gene            716..1633
                     /locus_tag="VF276_02405"
     CDS             716..1633
                     /locus_tag="VF276_02405"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6605744.1"
                     /translation="MSRIGIGWFLPNGPESPEARATFVADVDRNLQAVAGHFTSAWMA
                     DHLQAEKYDMLEAWTTLTYYAARHPQLLFGHAVLCQSYRNPALLAKTAATFQYLTGGR
                     LVFGLGAGWKEDEYLAYNYPFPSPGTRVDELEEALQIVKAMWTQEQATFEGQHYQIHA
                     AYCEPKPDPIPPIMIGGWKPRMLHLIARYADWWDVSGFGLPLANYPGFAAGMDRACAE
                     VGRDPRTLRRTFSTSCVCAPTEEAVQTLAEGLRPGRGFVGTPAQIVEQMRPFLDLGVD
                     HFQLAFHGFPDTAPVELFIAEVLPALEAL"
     gene            2113..3315
                     /locus_tag="VF276_02410"
     CDS             2113..3315
                     /locus_tag="VF276_02410"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6605745.1"
                     /translation="MIRYFRSPRAGAGFLLAVLVAALTLSGCDTPGASSAAAPIMIGG
                     LFNVTGPMGGLDVEGNNGALLAAQEINAAGGVLGTTINLVVKDGKTDPTTIGQVTADL
                     LKDHPAALVGFGDTDSVLISGPFAQRANVPFVTYGATSPRLPVTVGSTLFLACFGDNV
                     QAAAGAEFMYNTLHARKVALVYDKGVEYTRLLAGYFKDSWTGLAGRDSILTEETYRYK
                     DTDYSVQIQHIKALKTQPDVLYIAAMPVDIPVIVKQLRDAGITLPVVGGDGYDTPDLL
                     KVGPAANNVYYSTHALVNAEQSSPALKSFTERYKAAYKTDPSAFSALGYDAMMLVADA
                     VKRAGSGDPVKVQKALEETTNFAGLTGSISFSASSHIPRKDVTMLAVKDGRLDLASEL
                     QPKVIPAP"
     gene            complement(3407..4150)
                     /locus_tag="VF276_02415"
     CDS             complement(3407..4150)
                     /locus_tag="VF276_02415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6605746.1"
                     /translation="MFANSVFDTPRAAYGAAPAPVPAANPAPLSMNEQARLIAAAQAD
                     PEAFAALYHRYYPVILRFARRRTRNTAQAEDVTAATFLAAWEALPRYEWRGIPFTAWL
                     YRIAATIIAGEYRKQHGVSYVPLDDPLAPAQDIPSGDPDPEAWLEAREQREYARALIR
                     RALPHLTPAQREAVTLRYAGPEPLPLAEVARRMGRTEGAVKLLLHRAMGALRRALADV
                     EEPGDPRPAHSHHRTHDVPSLPYDTLEAA"
     gene            complement(4356..>5010)
                     /locus_tag="VF276_02420"
     CDS             complement(4356..>5010)
                     /locus_tag="VF276_02420"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX6605747.1"
                     /translation="MRAHEVPAEASSVRVARLLRTLATGDPAVWHHGQRVAWFAIRIG
                     ATLDVDLAQLYLAGLLHDIGKLALPAELLAKPDALTPAEQTWMRMHPDLGAAVLPQDR
                     SLAVVYAGVRYHHERWDGAGYPTGLRGEAIPLAARILAIADSLDAICMDRPYRPRRPF
                     SVALDTVSRAAGQQFDPALVDIAVRALAKNPAPPRPAAPPAPAFALPLAATTPAFIN"
BASE COUNT          771 a         1700 c         1749 g          790 t
ORIGIN      
        1 ctcggtcagc ccgaacgcgc cgatgaggtc catgcgcgcc aggcccggca gccagcgctg
       61 tttctgcgcc tcggagccgc aataatagat gctggacatc gccagcccgc tggtgacgcc
      121 gaaaaaggtg cacagcgagg cgtcgccgcg agccagttcc atgctcgcca gcccggtcgc
      181 cacgctggac aggcccggac agccgtagcc ctggagcacc ccgccgcaga tgcccagcgc
      241 ggccagtttg gggatcaggt gcgccgggaa ctcggcccgt tcccagtagg ggttgatgat
      301 gggcgtgact tcggcgtcga caaaggcgcg gacgcggtcg cgcacgtcgc gctcctgggg
      361 cgtcagcagc ccatccaggt cgtagaaatc gcgcgccggg tccaccgcgg gccgtgcccc
      421 ctcgggtgcc ccggcgctgc cgtccgcctc ggcttgcaac gccatgtacg tttctcctgt
      481 tctctccgcg aataggccgg tgcagatcgc gcctgtagac gcgccccgcc ccttgattat
      541 aaccaatccg ggcgtatccg gcccggagtg cctggtcgat gtgttgcccg gtctaccgtg
      601 cgccgcttgc cgggccgggt ataatggcga tcaggcgtct tgtttgcggt gcccgatcca
      661 cgacggcgcc cggcgtctgc cgtatcgatc ccaagcgcag cagaggagtt cgcacatgag
      721 tcgcattggc attggctggt ttttgcccaa cggcccggag tcgcccgaag cgcgggccac
      781 gttcgtcgcg gatgtcgatc gcaatctcca ggccgtggcc gggcatttca cgtcggcctg
      841 gatggccgac cacttacagg ccgaaaagta cgacatgctg gaagcctgga ccacgctgac
      901 atattacgcc gcgcgccacc cgcaattgct gttcggtcac gcggtgctgt gccagtcgta
      961 tcgcaacccg gcgctgctgg cgaaaacggc ggccaccttc cagtacctga ccgggggtcg
     1021 cctggtgttc gggctgggcg cgggctggaa ggaggacgag tacctggcct acaactaccc
     1081 cttcccgtcg cccggcacgc gcgtggacga gttggaggaa gcgctccaga tcgtcaaggc
     1141 gatgtggacg caggagcagg cgaccttcga gggccaacac taccagatcc acgccgcgta
     1201 ctgcgagccg aagcccgacc cgatcccgcc catcatgatc ggcggatgga agccgcgtat
     1261 gctgcacttg atcgcccggt atgccgactg gtgggatgtc tccggcttcg gtttaccgct
     1321 cgccaactac cccggcttcg ccgccgggat ggaccgcgcc tgtgctgagg tggggcggga
     1381 cccgcggacc ttgcgccgaa cgttttcgac atcctgtgtt tgcgcgccga cagaagaggc
     1441 ggtgcagacg ctcgcggaag gactgcggcc cggtcgcggc ttcgtcggca ccccggcgca
     1501 gatcgtggag cagatgcgcc ccttcctcga cctgggcgtc gatcacttcc aactggcgtt
     1561 ccacggcttc cccgataccg cgcccgtcga gttgttcatc gccgaggtgc tgcccgcgct
     1621 ggaagcactg tagcggggaa acccgacggg gcgagaccgg cgcggatgct caggccgcgc
     1681 gggtctcgcc ccgtcttttt gcgcgggtgc gtcgcgccgg cgacgatagg tgcttgtctc
     1741 tccgcgattt tgccggtgct cacccctttg ctctggtgga cggcgccatc cagtcccgcg
     1801 cgcctcacaa cggccccgat ggggcgctgg aaaccgggac taccggtccc ggcggttggg
     1861 acctgggcca ctagcaagcg ggtgtgcatc caccgtagaa ttgggctcaa tccaggtgta
     1921 gcgctgggcg tgggccggtt gggggaccgc cgccggatgc gccacctgat cgatgggcgc
     1981 atacccccag ccgtcgccct ccttccccgc cggtggccgg cggcgacagg gccgcggaga
     2041 gcgcgtgctg gacaccgccg cacgcctcct acgtcgcaac cagcttcgtg cgaaccttgt
     2101 aaggagtagt ccatgatccg gtatttccgc agcccccgcg ccggcgcggg ttttttgctg
     2161 gcggtcctcg tcgccgcgct gaccctgagc gggtgcgata cgcccggcgc cagcagcgcc
     2221 gccgccccga ttatgatcgg cggcctgttt aacgtcacgg gtcccatggg gggcctggat
     2281 gtcgagggca acaacggcgc cctgctggcg gcccaggaga tcaacgccgc gggcggcgtg
     2341 ctgggcacca ccattaacct ggtcgtcaaa gacggcaaga ccgatccgac gaccattggg
     2401 caggtcaccg ccgatttact gaaggatcac ccggcggccc tggttgggtt cggcgatacc
     2461 gactcggtgc tcatctccgg ccccttcgcc cagcgggcca acgtgccgtt cgtcacctac
     2521 ggcgccacct cgccccgcct gcccgtgact gtcggctcga cgctcttcct cgcctgcttc
     2581 ggcgacaacg tccaggccgc ggcgggcgcc gagttcatgt acaacacgct gcacgcacgc
     2641 aaggtcgccc tcgtctatga caagggcgtt gagtataccc gcttgctggc cggctatttc
     2701 aaggacagtt ggaccgggct ggccgggcgc gactcgatcc tgaccgagga aacctaccgc
     2761 tacaaggaca cggactacag cgtgcagatc cagcacatca aggcgttgaa gacgcagccg
     2821 gacgtgctct atatcgcggc gatgcccgtc gatattccgg ttatcgtcaa gcaattgcgc
     2881 gacgccggga tcacattgcc ggtggtgggc ggcgacggct acgatacgcc cgacctgctg
     2941 aaagtggggc cggcggccaa caacgtctac tacagcactc acgcgctggt caatgccgag
     3001 cagtccagcc ccgcgctcaa gtcgttcacc gagcgctaca aggcggccta caagaccgac
     3061 cccagcgcct tttcggccct gggctatgat gccatgatgc tggtggccga cgccgtcaag
     3121 cgcgcgggca gcggcgaccc ggtcaaagtc cagaaagcgc tggaagagac gacgaacttt
     3181 gcgggcctta ccggcagcat ctccttcagc gcctccagcc acatcccgcg caaggacgtg
     3241 acgatgctgg cggtcaagga cggccggctc gacctggcct cggagttgca gcccaaggtt
     3301 attccggcgc cctaagcggc gcgggccggt ttcaaaccgg cccctacagc agaaacgcga
     3361 cgcgcccggc agatgccggg cgcgttgctg tgttctggtc ttggggtcaa gcggcttcca
     3421 gcgtgtcata cggaagcgat ggcacgtcgt gcgtccggtg atgtgagtgg gccgggcggg
     3481 gatcaccagg ctcctcgaca tcggccagcg cgcggcgcag cgcgcccatg gcgcggtgca
     3541 gcagcaactt gaccgcgccc tcggtgcggc ccatgcgccg ggcgacctcg gccagcggca
     3601 gcggttcggg gccggcgtag cgcagggtca cggcctcgcg ctgggccggg gtcaggtggg
     3661 gcagggcgcg ccggatgagg gcgcgggcgt actcgcgctg ctcgcgggct tccagccagg
     3721 cttcggggtc ggggtcgccg gacgggatgt cctgcgccgg ggccagcggg tcgtcgagcg
     3781 gcacatagct cacgccgtgc tgcttgcggt actcgcccgc gatgatggtg gccgcgatgc
     3841 ggtagagcca ggcggtgaac gggatgccac gccactcgta gcgcggcagg gcttcccacg
     3901 cggccaggaa cgtggcagcg gtcacgtcct cggcctgggc cgtgttgcgc gtgcggcgcc
     3961 gggcgaagcg caggatcacc gggtagtagc gatggtagag tgcggcaaac gcctcggggt
     4021 ccgcctgcgc cgcggcgatc aggcgcgcct gctcattcat ggacagcggc gccggattgg
     4081 cggcggggac gggggccggg gcggcgccat aggcggcgcg gggcgtatcg aaaacagaat
     4141 tcgcgaacat caatcggctc cttgacaaca ggctccccgg cagctagtag tgcaccacac
     4201 taattcttat gggcggcagg ccggtttgaa accggcccct acaccgcaaa atcaccgtgg
     4261 tcgcctatca gggcgctgcc gggcgagcgc aaacccatca gggtggagcg ggcgcggggc
     4321 ggccacgcgc ccccgccggc ccgacaacaa gtgtcttagt tgatgaaggc cggagtggtc
     4381 gccgccaacg gcagggcgaa ggccggggcc ggcggagcgg cggggcgcgg cggcgccggg
     4441 ttcttggcca gggcgcgcac cgcgatgtcc accagtgccg ggtcgaactg ttggcccgcc
     4501 gcccggctca ccgtgtccag cgccacgctg aacggccgcc gcggccggta gggccggtcc
     4561 atgcagatcg cgtcgagcga gtcggcgatg gcgaggatgc gtgcggccag cgggatggcc
     4621 tcgccgcgca gccctgtcgg atagcccgcg ccgtcccagc gctcgtggtg gtagcgcacg
     4681 cccgcgtaga ccaccgccag gctgcggtct tgcggcagca cggcggcgcc caggtcggga
     4741 tgcatgcgca tccaggtttg ctcggcgggg gtcagcgcat ccggcttcgc cagcagctcg
     4801 gcgggcagcg ccagcttgcc gatatcgtgc agcagcccgg cgaggtagag ttgcgccagg
     4861 tccacgtcca gcgtggcgcc gatgcggatc gcgaaccagg cgacccgctg cccgtggtgc
     4921 cagacggcgg gatctcccgt cgccagcgtg cgcagcaggc gcgccacgcg gaccgaggag
     4981 gcttcggcgg gcacctcgtg ggcgcgcatc
//
LOCUS       DASUYC010000151         2309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1519052, whole genome shotgun sequence.
ACCESSION   DASUYC010000151 DASUYC010000000
VERSION     DASUYC010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1519052"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="VF276_02425"
     CDS             complement(<1..550)
                     /locus_tag="VF276_02425"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX6605748.1"
                     /translation="MQWAELTGDQFPEAVAQSGGVCLVPLSVLERHGHHLPLATDTYI
                     ARELCRRAAAREPAIVFPDYIFTQIPEARHLAGTISIDGDLMVRLLDNICREIARNGC
                     KKIVLVNAHGGNHGLLSLFNMLQLAGPRDYVVYVVQPVEMMFGGTLETPWDPTSDGHA
                     GPGETSMILALRPDLVDMGRVPA"
     gene            complement(571..>2309)
                     /locus_tag="VF276_02430"
     CDS             complement(571..>2309)
                     /locus_tag="VF276_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005480355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX6605749.1"
                     /translation="RPIEERVADLLAQMTLEEKAGMLFHTMIMMGADGSLVEGAGMFG
                     PFSTRDMVVGRCMNHFNILRGAGPRQMAAWYNRLQELAEDTRLGIPITISSDPRHAFS
                     DNPATSFLSGAFSQWPEPLGLAATGDAALVEQFGDIARQEYSAVGIRVALHPMADLAT
                     EPRWARSNGTFGEDAELSARMTAAYIRGFQGATLGPQSVACMTKHFPGGGPQKDGEDP
                     HFPYGREQVYPGDNFDYHLIPFEAAFAAGTAQIMPYYGMPVGTPLEEVGFGFNKDVIT
                     GLLRDKFGFDGVVCTDWGLLTDSEIGGAPFPARAWGVEHLSRPERAKKILDAGCDQFG
                     GESCPEVVVELVRSGQIPESRLDQSVRRLLRDKFRLGLFDNPYVDPDTAERVVGKAEF
                     RAAGEQAQRRSIVLLKNDNTSNGPTLPLTGRPRIYVENLDPAVAATYGEVVPTPEVAE
                     LAILRLKAPYEQREGNFLERLFHAGDLDFKEPEKSRILGLLAQVPTVVDIYLDRAAVI
                     PEIAAAAAALLADFGASDAAVLDVIFGRSAPSGRLPFELPSSMDAVRRQYPDVPRDSA
                     NPLFPFGYGLTY"
BASE COUNT          361 a          775 c          801 g          372 t
ORIGIN      
        1 ccgccggcac ccggcccata tccaccagat cgggccgcaa agccaggatc atgctcgttt
       61 cgcccggccc ggcatggccg tcgctggtgg ggtcccaggg cgtctccagc gtccccccga
      121 acatcatctc gacgggttgc accacgtaga cgacgtagtc gcgcgggccg gccagttgca
      181 gcatgttgaa cagcgacagc aggccatggt tgccgccgtg ggcgttcacc agcacgatct
      241 tcttgcagcc gttgcgggcg atctcgcggc agatattgtc gagcaggcgc accatcaggt
      301 cgccgtcgat gctgatcgtg ccggccaggt ggcgcgcctc cgggatctgg gtgaagatat
      361 agtcgggaaa cacaatcgcc ggttcgcggg ccgcggcccg ccggcacaac tcgcgggcga
      421 tatacgtgtc ggtcgccagc ggcaggtggt gaccgtggcg ctccagcacc gacaggggca
      481 ccaggcagac gcccccgctc tgcgcgacgg cttcgggaaa ctggtcgccg gtcaattcgg
      541 cccattgcat ggcggcgcgc tcctttcgcc tcagtaggtc aggccgtagc cgaacgggaa
      601 cagcggattg gccgagtcgc ggggcacatc cgggtactgc cggcgtaccg cgtccatcga
      661 cgacggcaac tcaaacggca gccggcccga cggcgcgctc cgcccgaaga tcacatcgag
      721 cacggcggca tcgctcgcgc cgaagtcggc cagcaacgcc gccgcggccg ccgcgatttc
      781 ggggatcacg gcggcacggt ccaggtagat gtccaccacg gtgggcacct gcgccagcag
      841 gcccaggatg cggctctttt ccggctcctt gaaatcgagg tcgcctgcgt ggaacaggcg
      901 ctccaggaaa ttgccctcgc gctgctcata gggcgccttc agccgcagga tcgctaattc
      961 ggcgacctcg ggcgtgggca ccacctcgcc gtaggtggcg gccaccgccg ggtcgaggtt
     1021 ttcgacgtag atgcggggcc gcccggtcag cggtaaggtc gggccgttgg aggtgttgtc
     1081 atttttcagc agcacgatcg accggcgctg ggcctgctcg cccgccgcgc ggaactcggc
     1141 cttgcccacc acgcgctccg ccgtgtcggg gtccacgtag gggttgtcga acaggccaag
     1201 ccggaacttg tcgcgcagca accggcgcac cgactggtcg aggcggcttt cggggatctg
     1261 cccgctgcgc accaattcga ccactacctc cggacaggat tcaccgccga actggtcgca
     1321 tccggcgtcg aggatcttct tcgcccgctc cgggcggctg aggtgctcga caccccaggc
     1381 gcgcgccggg aacggggcgc cgccgatctc ggagtcggtc agcaggcccc agtcggtgca
     1441 gaccacgccg tcgaagccga acttgtcgcg cagcaggccg gtgatcacgt ccttgttgaa
     1501 gccgaagccg acttcttcga gcggtgtgcc gaccggcatg ccgtagtagg gcatgatctg
     1561 ggcggtgccc gccgcaaagg cggcctcgaa cggaatcagg tgatagtcga agttgtcgcc
     1621 gggatagacc tgctcgcggc cataggggaa atgcgggtcc tcgccgtctt tctgcgggcc
     1681 gccaccaggg aaatgcttgg tcatgcaggc gacgctctgc gggccgagcg tagcgccctg
     1741 gaagccgcgg atgtaggcgg cggtcatgcg cgcggacagc tcggcgtctt cgccgaaggt
     1801 gccgttgctg cgcgcccagc gcggctcggt ggcgaggtcg gccatggggt gcagggcgac
     1861 gcggatgccg acggcgctgt actcctgccg ggcgatatcc ccgaactgct ccaccagcgc
     1921 cgcgtcgccg gtggcggcca ggcccagtgg ttcgggccac tgcgagaagg cgccggacag
     1981 gaagctggtc gccgggttgt cgctgaaggc gtggcgcggg tcggacgaga tcgtgatcgg
     2041 gatgccgagc cgggtatcct cggccagttc ttgtagccgg ttgtaccatg cggccatttg
     2101 ccgggggccg gcgccacgca gaatattgaa gtggttcatg caccgcccga ccaccatgtc
     2161 cctggtcgag aacgggccga acatgcccgc gccttccacc aggctgccgt ccgcgcccat
     2221 catgatcatc gtatggaaca gcatcccggc cttctcctcc agggtcatct gggcgagcag
     2281 gtcggcgaca cgctcctcga tgggccggc
//
LOCUS       DASUYC010000152         2729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_600245,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000152 DASUYC010000000
VERSION     DASUYC010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600245"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..636
                     /locus_tag="VF276_02435"
     CDS             217..636
                     /locus_tag="VF276_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX6605750.1"
                     /translation="MPDRNQPIIDEFRANNGKVGGYFEGANMLLLHTAGAKTGQARTN
                     PLIYVTDGDRLVIIASKGGAPSNPDWYYNVLANPLVTVELGNEQFQARATPVTDEPER
                     SRLYARMVEHRPDFAEYERKTSRKIPAIILERVSYPA"
     gene            complement(767..>2729)
                     /locus_tag="VF276_02440"
     CDS             complement(767..>2729)
                     /locus_tag="VF276_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEX6605751.1"
                     /translation="FAPAQASYLADPPGATAQFASPLFERVWSRTDAPVAANIAHRSW
                     TWGPGPGPTREEPFAGAPNGTRQVQYFDKGRMEVNPAVKDPNSPWAATSGLLVVEMVS
                     GQIQTGPNQFEARGPADVPVVGEPPAPGDVRVPTYRSFASVASLPGGPDRRAPVNKKI
                     VVGTIDRDGAGQRPLTKDINVSNVAYVPETGHNVPDVFWSFMEAHGLVLTGGVYDQRQ
                     LFDPVFVLGYPITEAYWTTAIINGKPTQVLVQLFQRRVLTFVPSFPPAWQVQMGNVGQ
                     HYYEWRYGQPLPEAAPVAVTNAPPLPPADAFVHVEGDQLVLRGQPITIKGTNYWLHDA
                     PFVETWAQWDAPTVKAELGKAHELGANTVRITLPYDYLSLRDTVWGDKASERINGPII
                     NLMTQFLQIAASYDIKVIFALFDATDRIEELGDADKRIQLNYVQGLVEPFANDDRVLA
                     WDLRNEPDNGVVGRRNPTLMIGWLNMIAQAVRSIDTRHPITVGLGNYENLWAAPRKLS
                     VLDFVDFASFHSYDAGRFADQIAAVKAHTKLPILVSEFGWPTGPAAESTAAATYDEPT
                     QQGLYRTMLGAARAAGIAGMMQWTLWDYGKDAPQRNYEAHFGLVRLDGSFKPAAADFR
                     DGYPAPPLPSVTRTNVPLTPHGVEPQIGP"
BASE COUNT          456 a          890 c          915 g          468 t
ORIGIN      
        1 tacgaaaagg gccagatcct gggcaacgcc cagcgccacc tgctggagtt ctacaaaacc
       61 ggcgacgcga acgtgatccg ctcggcgcgg tagcggtcga ccgagctaag cggggcacag
      121 agtttgcgac ccgttacttg acgccatcgt ctacaggcgg tggcctcttt acttgataga
      181 caaaccctac gcggccacct ctcgaaagag gatgtcatgc ctgatcgtaa tcagcccatc
      241 atcgacgagt ttcgggccaa taacggcaaa gtcggcgggt acttcgaggg cgccaacatg
      301 ctcctgctgc acacggccgg tgccaagacc ggccaggccc gcacgaatcc gctgatttac
      361 gtcaccgacg gtgatcgcct cgttatcatt gcgtccaaag ggggcgcgcc cagtaatccc
      421 gactggtact acaatgtact agccaacccg ctcgtgacgg ttgaactcgg caacgaacag
      481 tttcaggcgc gggcgacccc tgttaccgac gaaccggagc gatcccgcct ctatgccagg
      541 atggttgagc accggcccga ctttgcggag tatgagcgaa agaccagccg caagattccc
      601 gccattatcc tggaacgagt aagctaccca gcctgagccg agcactgctg ctacgaaccg
      661 caaagtaaag agccgccggc ctctttcggg cgcgggcggc tttttactat atcctgatag
      721 ctgatcgcgc agcggtgtgt agcacacggc tgaccgctga ccgctctcat ggcccgatct
      781 gcggctccac gccgtggggc gtcaggggca cgttggtgcg ggtgacgctg ggcagcgggg
      841 gcgccgggta gccgtcccgg aagtcggcgg cggcgggctt gaacgagccg tccagccgca
      901 ccaggccgaa gtgggcctcg tagttgcgct gcggcgcgtc cttgccgtag tcccacaacg
      961 tccactgcat catcccggcg atgccggcgg cgcgggccgc gccgagcatc gtccggtaca
     1021 ggccctgctg cgtcggctca tcgtaggttg cggcagcggt ggactccgcc gccgggccgg
     1081 tgggccagcc gaactcgctg accaggatgg gcagtttggt gtgcgccttg accgccgcga
     1141 tctgatcggc gaagcggccc gcgtcgtagc tgtggaagct ggcgaagtcg acaaagtcga
     1201 gcacagacag cttgcgcggc gcagcccaca ggttctcgta gttgcccagg ccgaccgtga
     1261 tcgggtggcg ggtgtcgatg gagcgcacgg cctgggcgat catgttcagc cagccgatca
     1321 tcagcgtcgg gttgcgccgc ccgacgacgc cgttgtcggg ctcgttgcgc agatcccagg
     1381 ccagcacccg gtcgtcgttg gcgaacggct ccaccaggcc ctgcacatag ttcagttgga
     1441 tgcgtttgtc cgcgtcgccc agttcttcga tgcggtcggt ggcgtcgaac agcgcgaaga
     1501 tgaccttaat gtcgtaggac gcggcgattt gcaggaactg ggtcatcagg ttgatgatcg
     1561 ggccgttgat ccgctcgctc gccttgtcgc cccagacggt atcgcgcagc gacagatagt
     1621 catagggcag cgtgatgcgc accgtgttgg cgccgagttc atgagccttg ccgagttcgg
     1681 ctttgaccgt tggcgcgtcc cactgagccc aggtctcgac gaagggcgcg tcgtgcagcc
     1741 agtagttggt gcccttgatc gtgatcggct gcccgcgcag caccaactgg tcgccctcga
     1801 catggacgaa ggcatcggcg ggcggcagcg gcggggcgtt ggtcaccgcc accggcgcgg
     1861 cttcgggcag cggctggccg tagcgccact cgtagtagtg ctggcccacg ttacccatct
     1921 gcacctgcca tgcgggcggg aagctgggca caaaggtcag cacacggcgc tggaagagct
     1981 gcactagtac ctgggtcggt ttgccgttga tgatcgcggt ggtccaatac gcctcggtga
     2041 tggggtagcc gagcacaaac accgggtcga acaactggcg ctggtcgtac acgccgccgg
     2101 tgagcaccag accatgggcc tccatgaagc tccagaacac atcgggcacg ttatggccgg
     2161 tctcgggcac ataggccacg ttgctgacgt tgatgtcctt ggttagcggg cgctgcccgg
     2221 cgccgtcgcg gtcgatggtg ccgacgacga tcttcttgtt caccggcgcg cgccggtccg
     2281 gcccgccggg cagcgaggcc acgctcgcga agctgcggta cgtgggcacg cgcacatcgc
     2341 cgggcgcggg cggttcgccc accaccggca catcggcggg gccgcgcgcc tcgaactggt
     2401 tcggcccggt ctggatctgc ccgctcacca tctccacgac aagcaaaccg ctggtcgccg
     2461 cccaggggct gttcggatcc ttgaccgccg ggttcacctc catgcggccc ttgtcgaaat
     2521 actgcacctg gcgggtgccg ttgggcgccc cggcaaacgg ctcctcgcgc gtcggccccg
     2581 gccccggccc ccaggtccat gaccggtggg cgatattcgc ggccacgggc gcgtcggtcc
     2641 gcgaccagac ccgctcgaac agcggcgacg cgaactgcgc cgtggcacca ggggggtccg
     2701 ccaggtagct cgcctgggcg ggcgcgaag
//
LOCUS       DASUYC010000153         3466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_791498,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000153 DASUYC010000000
VERSION     DASUYC010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791498"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..3038)
                     /gene="ppdK"
                     /locus_tag="VF276_02445"
     CDS             complement(159..3038)
                     /gene="ppdK"
                     /locus_tag="VF276_02445"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEX6605752.1"
                     /translation="MTSTANAATTPGSATSPNGQDGAQTHKWVYLFPEGNGKMKDLLG
                     GKGAGLSEMTNAGLPVPPGFTITTEACRAYYENGEQFPPGMWDQALAALHTVEEQTGK
                     KLGDPANPLLVSVRSGAKFSMPGMMDTVLNLGLNEQTLQGLVKLTGNERFGYDAYRRF
                     IQMFSKIVLDIPAERFEKKLDELKERVGAHYDTDLTAENLRDLVSEFKAIVREASGKE
                     FPDDPLEQLRLAIEAVFASWNNRRAITYRDLNKIPHDLGTAVNVVTMVFGNMGNDSGT
                     GVAFTRNPSTGESQLYGEFLMNAQGEDVVAGIRTPQKISQLREVLPEVYNQFEQIGKR
                     LESHYRDAQDLEFTVERGKLYMLQTRSAKRTAAAAVKMAVDMANDGLISKEEALQRVE
                     PAQVVQLLLPRFDPAATEAARQAGRLLAKGLNASPGAASGKAVFDADRVAEMGQAGEA
                     VILVRQETNPDDVHGMIAARGILTARGGATSHAAVVARGMGKPCVAGTESIKVDAEAR
                     QFTVDGKVIKEGDEISIDGTTGEVFAGQIPTTAASFKDEKELVQLLKWADEVRKLGVW
                     ANADYPRDAERAIEFGAEGIGLCRTEHMFFEEVRLPIVRRMILAAADATRLLNARDRA
                     QQALDATSSDRRGDAEAALQAAQQAADSSDVVQDYLGALGELEQFQTSDFEGILGAMG
                     EKPVVVRLLDPPLHEFLPSHDELLAEISEMRGAGKTDSDEYREKEHLLHVVEGLREQN
                     PMLGLRGDRLGILYPAIYEMQVRALLNAAARLTKSGKKVHVEIMIPLTGTEKELEVMR
                     KLVDRAAAEVEKAQGGPVAFKYGTMIEVPRAALVAGKLAQSAEFFSFGTNDLTQTTFG
                     YSRDDAEGKFLLQYVEMGVLPVNPFQTLDRDGVGRLIEIAVKEGRQTRPDLKVGICGE
                     HGGDPDSIAFCNQAGLNYVSCSPFRVPVARLAAAQAVLTTTERDR"
     gene            complement(3350..3454)
                     /locus_tag="VF276_02450"
     CDS             complement(3350..3454)
                     /locus_tag="VF276_02450"
                     /inference="COORDINATES: protein motif:HMM:NF044424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605753.1"
                     /translation="MQSKPTGGDEEVTIAFAGHGIKRLLASLANLERL"
BASE COUNT          490 a         1107 c         1235 g          634 t
ORIGIN      
        1 tgcgccgttg cagcagcagg cgccctgcgg cgtcgaagat ggccgcggac gcgcctgcgc
       61 gcatctggtt gggcgggccg ccgtaagtgg tctggtcggg catagaggaa ggggagaaaa
      121 agaggagggg cgccgcaggg cgcccctccg ggtgttgctt agcggtcgcg ctcggtggtg
      181 gtcagcaccg cctgggccgc cgccaggcgg gcgacgggca cgcggaacgg gctgcacgag
      241 acgtaattca gaccggcctg gttgcagaag gcaatcgagt cggggtcgcc gccgtgctcg
      301 ccgcagatgc ccaccttgag atcggggcgg gtctggcggc cttccttcac ggcgatctcg
      361 atcaggcggc ccacgccgtc gcggtcgagc gtctggaacg ggttgaccgg cagcacgccc
      421 atctcgacat actgcaacag gaacttgccc tcggcgtcgt cgcggctgta gccgaacgtc
      481 gtctgggtca ggtcgttggt gccgaaggag aagaactcgg cggattgggc cagcttcccg
      541 gcgaccagcg cggcgcgcgg cacctcgatc atggtgccgt acttgaaggc gaccggcccg
      601 ccctgggcct tctccacctc ggcggcggcg cggtcgacca gcttgcgcat gacttccagc
      661 tctttctccg tgccggtgag cgggatcatg atctcgacgt ggaccttctt gccgctcttg
      721 gtcagcctgg cggcggcatt gagcagcgcg cgcacctgca tctcgtagat ggccgggtag
      781 agaataccga ggcggtcgcc gcgcaggccc agcatggggt tctgctcgcg caacccttcg
      841 acgacgtgca gcaggtgctc cttctcgcgg tactcgtcgg aatcggtctt gcccgcgccg
      901 cgcatctcgc tgatctcggc cagcagttcg tcgtgcgagg gcaggaactc gtgcagcggc
      961 gggtcgagca ggcgcaccac gacgggcttc tcgcccatgg cgcccaggat gccttcgaag
     1021 tcgctggtct ggaattgctc caactcgccg agcgcgccca ggtagtcctg gacgacatcg
     1081 ctgctgtcgg cggcttgctg cgcggcctga agcgccgctt cggcatcgcc gcggcggtcg
     1141 ctggaggtgg cgtccagcgc ttgctgcgcc cggtcgcggg cgttgagcag gcgggtggcg
     1201 tcggcggcgg ccaggatcat gcggcgcacg atgggcaggc gcacttcctc gaagaacatg
     1261 tgctcggtgc ggcagaggcc gatgccctcg gcgccgaact cgatggcgcg ctcggcgtcg
     1321 cgcgggtagt cggcgttggc ccagacgccc agcttgcgca cctcgtcggc ccacttcagc
     1381 agttgcacca gttccttctc gtccttgaac gaggcggcgg tggtcgggat ctgcccggca
     1441 aagacctcgc cggtggtgcc gtcgatggag atttcgtcgc cttccttaat taccttgccg
     1501 tccacggtga actggcgggc ctcggcgtcg accttgatcg actcggtgcc ggccacgcag
     1561 ggcttgccca tgccgcgggc gaccacggcg gcgtggctgg tggccccgcc acgggcggtc
     1621 aggatgccgc gggcggcgat catgccgtgc acgtcgtcag ggttggtctc ctggcgcacc
     1681 aggatcacgg cctcgccggc ctggcccatt tcggccacgc ggtcggcgtc gaagacggcc
     1741 ttgccgctgg cggcgccggg ggaggcgttg aggcccttgg cgagcaggcg gcccgcctgg
     1801 cgcgcggcct cggtggccgc cgggtcgaag cgcggcagca gcagttggac gacctgcgcc
     1861 ggttcgacgc gctgcaacgc ctcttccttg ctgatcagcc cgtcgttcgc catatcgacg
     1921 gccatcttga cggcggcggc ggcggtgcgc ttggccgagc gggtctggag catgtagagc
     1981 ttgccgcgct ccacggtgaa ctccaggtcc tgggcgtcgc ggtagtggct ctccagccgc
     2041 ttgccgatct gctcgaactg gttgtagacc tcgggcaaca cctcgcgcag ttgcgagatc
     2101 ttctgcggcg tgcggatgcc ggccaccacg tcctcgccct gggcgttcat caggaactcg
     2161 ccgtatagtt gcgattcgcc ggtggacggg ttgcgggtga aggctacgcc ggtgccgctg
     2221 tcgttgccca tgttgccgaa gaccatggtc acgacgttga cggcggtgcc caggtcgtgc
     2281 gggatcttgt tcaggtcgcg gtaggtgatg gcgcggcggt tgttccagga ggcgaacaca
     2341 gcctcgatgg cgagccgcaa ctgctccagc ggatcgtcag ggaactcctt gccgctggct
     2401 tcgcggacga tagccttgaa ctcgctcacc aggtcgcgca ggttctcggc ggtcaggtca
     2461 gtgtcgtagt gggcgccgac gcgctctttc aactcgtcca gcttcttctc gaagcgctcg
     2521 gcggggatgt cgagcacgat cttggagaac atctggatga agcggcggta ggcgtcatag
     2581 ccgaagcgct cgttgccggt cagcttgacc aggccctgga gcgtttgctc gttgaggccc
     2641 aggttgagca ccgtgtccat catgccgggc atcgagaact tggcgcccga gcgcaccgag
     2701 acgagcagcg ggttggccgg gtcgcccagc ttcttgccgg tttgctcctc gaccgtgtgc
     2761 agggcggcca gggcctggtc ccacatgccg ggcgggaact gctcgccgtt ctcgtagtag
     2821 gcgcggcagg cctcggtggt gatggtgaag cccggcggca ccggcagccc ggcgttggtc
     2881 atctcagaca gcccggcgcc cttgccgccg agcaggtcct tcattttgcc gttgccttcg
     2941 gggaacaaat agacccactt gtgggtttgc gcgccatcct ggccgttcgg gctggtggcg
     3001 ctgcccggtg tggtcgctgc gtttgcggtt gaagtcatgg agaatctcct tggcgggcac
     3061 gaggcccata ttccgatacc tggtgcggag caggtcaatc tgtgcggggc acgcgacccc
     3121 gcggacgtcc ttccgattct agcaaagaat cgcgaccctg gcaaggcgac gccctgctcc
     3181 cgaaatcgac accttactat accatacgaa aatcacagta ggggtatacg tccggtgaca
     3241 gcttggtgag aattggcgaa gcacgcggct gtacgcccgg cgcccgctgt gagacgacgg
     3301 acgccgggct acggtcttgc ggcgggatgg cgcaggcgga ttgcgaaccc tagagccgtt
     3361 cgagattcgc caggctggca agcaggcgct tgataccgtg gcccgcgaag gcgatggtga
     3421 cttcctcgtc gccgccggtg ggcttgctct gcacgacgat gccctc
//
LOCUS       DASUYC010000154         2588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1496721, whole genome shotgun sequence.
ACCESSION   DASUYC010000154 DASUYC010000000
VERSION     DASUYC010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1496721"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="VF276_02455"
     CDS             complement(<1..852)
                     /locus_tag="VF276_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX6605754.1"
                     /translation="MSQVSPDPALLVARPTLKTRTFHLSRDRLISIALITPSIIAVAV
                     FVYIFIAQTAYISLVRWNDLAPDFTWVGLYNYTRLFQTERFQIDLRNTFVFTVAFIAA
                     CLALGLLLATLLDMRIKFEAVFRNIFMFPLAVSFIVTGVAWRWLETPSSGINLLLDYA
                     GLGFLKSGWFTDPKIGILAVVLAAVWQMSGYVMAMYLAGLRGIPDELREAARVDGATE
                     WQIFRRVILPLLQPITLSAVIILGHISLKIFDLTKSMTGPGPGFSSDVPALFMYDTTF
                     SGSHFSQG"
     gene            complement(932..2242)
                     /locus_tag="VF276_02460"
     CDS             complement(932..2242)
                     /locus_tag="VF276_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006677685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6605755.1"
                     /translation="MNRKRTLWLMSLLAVVAMLLTACGGETPTATAVPAAGSSGGQTA
                     AGGGKLEMFSWWTTGGEEAGLKAMYALYKKDHPNVEIVNQAVAGAAGADAKAVLKNRM
                     IGGDPPDAFQVHMGHELLDGYVAAGQVEPLDDLYKSEGFDKAFPKGVLDIVSGDGHYW
                     SVPVNIHRANVLWYNKKVFADNNLTAPVTFDDFFKVADALKAKGITALALGDKVTFAS
                     AHLFETVLLGTLGPDGYKGLWTGATKWDDPKVTDALNTFKKMLGYINSDHAALTWDGA
                     NDLVISGKAGMTIMGDWTDADYKAKKFTDYGWTNSPGTAGIYDALSDTFALPKNAKDR
                     DNALAWLKLAGSREGQDAFNPLKGSIPARTDAGQGDYDAYLKSAMGDWKKDIIVPSVV
                     HGAAAKDGWAQSYTDAVNSFVTNQDVAATQKALGQACVDAGICK"
BASE COUNT          422 a          792 c          920 g          454 t
ORIGIN      
        1 gccctgcgag aaatggctgc cgctgaaggt ggtgtcgtac atgaacaggg ccggcacgtc
       61 gctggaaaag ccggggccgg ggccggtcat cgacttggtc aggtcaaaga tcttgagcga
      121 gatgtggccg agaataatca ccgcgctgag cgtgatcggc tgcaacaggg gcaggatcac
      181 ccggcggaag atctgccact cggtggcgcc gtccacgcgg gccgcctcgc gcaactcgtc
      241 ggggatgccg cgcaggccgg ccaggtacat cgccatcaca tagcccgaca tctgccagac
      301 cgccgccagc acgacggcca ggatgccgat tttggggtcg gtgaaccagc ccgacttcaa
      361 gaagcccagc cccgcatagt cgagcagcag gttgatgccc gacgacggcg tctccagcca
      421 gcgccaggcc acgccggtca cgatgaacga cacggccagc gggaacatga agatgttacg
      481 gaacaccgcc tcgaacttga tgcgcatgtc gagcagcgtc gccagcagca ggcccagggc
      541 caggcaggcg gcgatgaagg ccacagtgaa gacgaacgta ttacgcaggt cgatctggaa
      601 gcgctcggtc tggaacaggc gcgtgtaatt atagaggccg acccaggtga agtcgggcgc
      661 caggtcgttc cagcgcacca gcgagatata ggcggtctgg gcgatgaaga tatagacgaa
      721 gacggcgacg gcgataatcg agggcgtgat cagggcgatg gagatcagcc ggtcccggct
      781 caggtggaag gtgcgggtct tcagggtggg acgggcgacg agcagcgccg ggtccgggct
      841 tacctggctc ataacgattc tcccgctggg gcagcgcggc ggccccgcag agccgccgcg
      901 cctgggggcc gtccgcggac ggccaaccgg cttacttgca gatgcccgcg tccacgcagg
      961 cctggccgag cgccttctgg gtggcggcca cgtcctggtt ggtcacgaag ctgttgaccg
     1021 cgtcggtgta ggactgcgcc cacccgtcct tggccgcggc gccgtggacc acgctgggca
     1081 cgatgatgtc cttcttccag tcgcccatgg ccgacttcag gtaggcgtca tagtcgccct
     1141 gcccggcgtc ggtgcgggcc gggatcgagc ccttgagcgg gttgaaggcg tcctggccct
     1201 cgcgcgagcc ggccagcttg agccaggcca gcgcgttgtc gcggtccttg gcgttcttgg
     1261 gcagggcgaa ggtgtcggac agcgcgtcgt agatgcccgc ggtgccgggg gagttggtcc
     1321 agccgtagtc ggtgaacttc ttggccttgt agtcggcgtc ggtccagtcg cccatgatcg
     1381 tcatgccggc tttgccggag atgaccaggt cgttggcgcc gtcccaggtc agggcggcgt
     1441 ggtcggagtt gatgtagccg agcatcttct tgaaggtgtt tagcgcgtcg gtgactttgg
     1501 ggtcgtccca cttggtggcg ccggtccaca ggcccttgta gccgtcgggg ccgagggtgc
     1561 cgagcagcac ggtctcgaac aggtgggccg aggcgaacgt gaccttatcg ccgagggcca
     1621 gcgcggtgat gcccttggcc ttgagcgcgt cggccacctt aaagaagtcg tcgaaggtga
     1681 cgggcgcggt caggttgttg tcggcgaaga ccttcttgtt ataccagagc acgttggcgc
     1741 ggtggatgtt caccggcacg gaccagtagt ggccgtcgcc ggagacgatg tcgagcacgc
     1801 ccttggggaa ggccttgtcg aagccctcgg acttgtagag gtcgtccagc ggctcgacct
     1861 gccccgcggc cacgtagccg tcgaggagtt catggcccat gtgcacctgg aaggcgtcgg
     1921 gcgggtcgcc gccgatcatg cggttcttga gcacggcctt ggcatccgcg cccgccgccc
     1981 cggcgacggc ctggttgacg atctcgacgt tggggtgatc tttcttatag agggcgtaca
     2041 tggccttgag gccggcctct tcgcccccgg tggtccacca ggagaacatt tcgagtttgc
     2101 cgcccccggc ggcggtctgg ccgccgctgc tgccggcggc gggcacggcg gtggcggtgg
     2161 gtgtctcgcc cccgcaggcc gtcagcagca tcgccaccac ggccagcagg ctcatgagcc
     2221 ataacgtgcg cttgcgattc atgcgattcc tcctttggaa aaccgaccct cctctagaac
     2281 cagggaccgc gggggtcccg ccagtgcggt gcgcggggtg gcgcctcctt tctctcgccc
     2341 tgccgagatc ggatacgact ctggtggcaa aacgctgccg tgcgctgttg tactggccgg
     2401 caccgggccg gccctgggtg tggttgtgga tgaacgatgc gaggatgaat cgccaccagg
     2461 atggggtagc aacagcgccc atcttacgcg agccgcgccg gtttgtcaag ggggtcatgg
     2521 cagcgcttac acgccgattt tgtgaccgct cgtattgccg caaaccgggc tgacagcagg
     2581 tgctttgc
//
LOCUS       DASUYC010000155         2862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1889535, whole genome shotgun sequence.
ACCESSION   DASUYC010000155 DASUYC010000000
VERSION     DASUYC010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1889535"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..1080)
                     /locus_tag="VF276_02465"
     CDS             complement(112..1080)
                     /locus_tag="VF276_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEX6605756.1"
                     /translation="MRAIRIQAFGGPEVLQVEEVPLPAPGPGEARVRIEAAGVNFIDI
                     YHRTGQYRGNLPLTLGQEGAGVVDAVGPDVSEVQVGERVAYASVQGAYAEYAIVPAAR
                     LVPVPDGVSSQVAAAVMLQGMTAHYLSHSTYPLGPQDTALVHAAAGGVGGLLVQIARR
                     RGARVIGTVSTEAKAAEARAAGADEVIRYTETDFVAETRRLTGGQGVPVVYDSVGKTT
                     FAGSLDCLRPRGYLVLFGQSSGAVPPLDPQVLNAKGSLFLTRPTLAHYIATRDELLWR
                     AGDLFGWIAAGELHVRVAATYTLSAAGEAQDYLAGRHAHGKVLLIP"
     gene            complement(1101..2687)
                     /locus_tag="VF276_02470"
     CDS             complement(1101..2687)
                     /locus_tag="VF276_02470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEX6605757.1"
                     /translation="MQNSSRYRYAIALTAALGLFMAVLDNTVVNVALTPMSSALQSSL
                     SSIQWVVTGYFLAQAAVIPIAGYFSTRFGIKRLFMICLSFFTVGSLLCGLSQNETMLI
                     GFRVLQGLGGGALFPLTQAIAFGAFPPRDRAAASAVVGIPVLLAPTFGPTIGGLLTDN
                     FGWEYIFFVNVPVGIVALALAWRIIPRDEPAPAGPRRSFDTIGLLLSVLGVLAVVYAF
                     TLVSQPQPGSETALNPRGTLYGWGYWPVWALLAAGAAILAGFIFYELRVSKDPVLDLR
                     LYRRRDFALGSLVTWVTAMIIFGSLILIPIFLEQVRVPHLSALDAGLALMPQGIAAAI
                     AVGLGGRLYNSIGVRNLIGLGAVCLFASSWMLTQLTPTTDGFGLWPALIVRGFGFGFT
                     NIPVQTLALQQITGPALPRASSLFTVTRQIFSSIGVAMVTTLFVQQTTQHADSLRASL
                     QGALPAGTAIDPNNPAFQAARDHLFAQAGTAGMNDVFTYVTAGTVLIVLLALALPNRA
                     QQAQAGATSGEQGQRPVLAE"
BASE COUNT          463 a          961 c         1050 g          388 t
ORIGIN      
        1 tctacgaacg ggcggcgatg ctgcgaacgg cgtgcccgga ccacgcctgg acggcgcagg
       61 acgtgagcca ggcgctctgg gcggcggcgg gcggcaaggc gggcgcggcg gctacgggat
      121 gagcagcacc ttgccgtgcg cgtggcggcc cgccagatag tcctgcgctt ccccggcggc
      181 ggacagggtg taggtcgcgg cgacgcgcac gtggagttct cccgccgcga tccagccgaa
      241 caggtcgccc gcgcgccaga gcagttcgtc gcgcgtggcg atgtagtggg ccagggtggg
      301 ccgggtcagg aagagcgacc ccttggcgtt cagcacctgc gggtcgagcg ggggcaccgc
      361 gccgctcgac tggccgaaca gcaccaggta gccgcgcggg cgcaggcaat ccaggctgcc
      421 ggcgaaggtc gtcttgccca ccgagtcgta gacaaccggg acgccctgcc cgccggtcag
      481 gcggcgggtc tcggccacga agtcggtctc cgtgtagcgg atcacctcgt cggcgcccgc
      541 ggcgcgcgct tcggcggcct tggcctcggt cgagacggtg ccgatgacgc gcgccccgcg
      601 ccgccgggcg atctgcacca gcaggccgcc cacgccgccc gccgccgcgt ggaccagcgc
      661 ggtatcctgc ggccccagcg ggtaggtgct gtggctgaga tagtgcgccg tcatgccctg
      721 gagcatgacc gccgcggcca cctggctgct gaccccgtcg ggcacgggca ccagccgcgc
      781 cgcggggacg atcgcatact cggcgtaggc gccctgcacc gaggcatagg ccacccgctc
      841 gcccacctgt acttcactca catcggggcc aacggcatcg accaccccgg cgccttcctg
      901 gccgagggtc agcggcaggt tgccgcgata ctggccggtc cggtgataga tgtcgatgaa
      961 gttgaccccg gcggcttcga tgcggacgcg cgcctcgccc ggtccgggcg ccggcagcgg
     1021 cacctcttcc acttgcaata cctccggccc gccgaaggcc tggatacgga ttgcccgcat
     1081 ggttcgcgct cctttccgag ctattcggcc agcacgggcc gctgcccttg ctcgccgctg
     1141 gtcgcgcccg cctgggcttg ctgcgcccgg ttgggcaggg ccagcgccag cagcacgatc
     1201 agcaccgtgc cggcggtaac gtaggtgaac acgtcgttca tgcccgccgt gccggcctgg
     1261 gcgaacaggt ggtcgcgggc ggcctggaac gccgggttgt tcggatctat cgccgtcccg
     1321 gcgggcagcg cgccctggag gctcgcgcgc aggctgtcgg cgtgctgcgt ggtctgctgc
     1381 acgaacagcg tcgtcaccat ggcgacgccg atggaactga agatctgccg ggtgacggtg
     1441 aacagggaac tggcgcgcgg cagggcgggg ccggtgatct gttgcagggc cagcgtctgc
     1501 accgggatgt tggtgaaccc gaagccgaag ccgcgcacga tcagcgccgg ccacaggccg
     1561 aacccatcgg tggtgggcgt cagttgggtg agcatccacg aactggcaaa caggcagacg
     1621 gcgcccagcc cgatcaggtt gcgcacgcct atgctgttat acaggcgccc gcccaggccc
     1681 acggcgatgg ccgccgcgat gccctgcggc atgagcgcca gcccggcgtc gagcgccgac
     1741 aggtggggca cgcgcacctg ttccaggaag atcgggatca ggatcaggct gccgaagatg
     1801 atcatggccg tcacccaggt gacgaggctg cccagggcga agtcgcgccg ccggtagagg
     1861 cgcaggtcca gcaccgggtc tttgctgacg cgcaactcgt agaagatgaa cccggccagg
     1921 atcgccgcgc ccgcggccag cagcgcccag accggccagt agccccagcc gtagagagtg
     1981 ccgcgcgggt tcagcgcggt ctcgctgccc ggctgcggct ggctgaccag ggtgaaggcg
     2041 tagaccaccg ccagcacgcc cagcaccgag agcagcagcc cgatggtgtc gaagctgcgc
     2101 cgcggcccgg cgggcgccgg ctcgtcgcgc gggatgatgc gccaggcgag ggccagggcc
     2161 acgatgccca ccggcacgtt gacgaagaag atatactccc agccgaagtt atcggtgagc
     2221 aggccgccga tggtcgggcc gaaggtcggc gccagcagca cggggatgcc gacgaccgcg
     2281 ctggccgccg cccggtcgcg gggcgggaac gcgccgaagg cgatggcctg ggtcagcggg
     2341 aagagcgcgc cgccgcccag cccttgcaac acgcggaagc cgatcagcat cgtctcgttc
     2401 tgcgacaggc cgcagagcaa cgagccgacg gtgaagaagc tcaggcagat catgaagagg
     2461 cgcttgatgc cgaagcgggt gctgaaatag cccgcgatgg ggatcaccgc ggcctgggcc
     2521 aggaaatagc ccgtcaccac ccactggatc gagctcaggc tgctctgcag cgcgctggac
     2581 atgggcgtga gggcgacgtt caccaccgta ttgtcgagca cggccatgaa cagaccgagc
     2641 gcggcggtga gggcaatggc ataccggtat cgagatgaat tttgcacgat tctctacctc
     2701 aaacctgtac gtgcggtggg catcctcgcg tcgcggacgc ataaagcgcc ccggtggcgg
     2761 agccggggcg gtgggcaggt atagatgtgg aagacgcgag gacggatcgg gcgcggcatc
     2821 ttcgtcgcga tattagagca tacggatgaa tcgtccgggc gg
//
LOCUS       DASUYC010000156         3732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1046615, whole genome shotgun sequence.
ACCESSION   DASUYC010000156 DASUYC010000000
VERSION     DASUYC010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046615"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..630
                     /locus_tag="VF276_02475"
     CDS             247..630
                     /locus_tag="VF276_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605758.1"
                     /translation="MTQLMWNPFEGQYRGQASTGEVVTVDEDVMNQAIKKAGSGVFEV
                     DWWIESRQDVLQHWGFELKRWVDPVTQMPRVNYALGAKGPAPQDEHGHTTSGILHKLT
                     SSGPLTTRILNTGKLNSGKLDEHQK"
     gene            complement(643..1698)
                     /locus_tag="VF276_02480"
     CDS             complement(643..1698)
                     /locus_tag="VF276_02480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEX6605759.1"
                     /translation="MPDSSNSTPRLPTSLDDIRTLLNTLNWTGMQREVQREAEERLAI
                     VGPVNSGKSTLFNLIAGKMVSQTSPIPGTTRTVIEQGLGPFTLIDTPGFGEAGGEDRA
                     ATALQGAATADALLVVFDAAAGLRQSDLQLMDALRRLGKPMVVVANKIDLVGKDAQSV
                     ETDMVRRLGVPVIAISAKKGTNVGERLLPALVDALPDLAVLLGRNLPSFRRTAANKVV
                     RNSMIINGGIGAEPIPFIDLPLLLANQARMVLRIAAIYGEPFNVHHARELIGTIAGGL
                     AFRYMAQQGARLVPTAGWAVSAGIAAIGTFAIGRVALEYFASGKRLTRREMRDMYKNM
                     LKRDRTTFEKDLAPRGK"
     gene            2322..3143
                     /locus_tag="VF276_02485"
     CDS             2322..3143
                     /locus_tag="VF276_02485"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009057 - macromolecule catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HEX6605760.1"
                     /translation="MSLPLAPAAPLLLIGGGSASQPVDMLHEFAKLAGGPGARIAVIT
                     SGSRAPQQVGARYERFFRSIDMEVGVYHLLSRAESNDPAVLQGFADAQAYFFTGGEQL
                     CITARMGGTEALRVLRRRHAEGAVLGGTSAGTSVMSETMIAFGNEGPVPSHSMVNLAP
                     GLGFLHGIILDQHFSQRQRLGRLKTAVSYNPEELGVGVDEDTAALFRPDGTLEVLGPG
                     TVTVVDELDVTDNTSPDVEDPDLPLCIEGVRIYQLSAGERFDCLGRSLAAIRAAA"
     gene            3329..>3732
                     /locus_tag="VF276_02490"
     CDS             3329..>3732
                     /locus_tag="VF276_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605761.1"
                     /translation="MTTRLPLRGVTGAVLALIFGLYLLTMSGHTYSPDEETMLATAEA
                     VGLHGTFALPRSRTLVEVPGLDGRFYSQYGPGQPVAAVPWIWAGHGVAALLGLDAAGQ
                     GFVVRFVLGSYNLLVAGGLAALLYAVAQRLGAG"
BASE COUNT          578 a         1238 c         1249 g          667 t
ORIGIN      
        1 cccccgcgtg tttggcttcc acgagatatt ccacctgttc gtcatcgcgg gcagcatcgc
       61 gttcgcggtg gcggtgtgcg catgggcgct gccgtacccg cgggcttagg gcgatcacac
      121 cctattcctc atagtactta atgcccatcc atgggtcatg ttttgcgctt ttgggcatat
      181 cccgatggta taatgcgccc aaagcggcct gaggtctcgc ttaggtaaca taggaagggc
      241 gcgccgatga cacaactgat gtggaacccg ttcgagggcc agtaccgggg ccaggcgtcc
      301 acgggcgagg tcgtcaccgt ggacgaagac gtgatgaacc aggctataaa gaaggcgggt
      361 tcaggcgtct tcgaagtcga ctggtggatc gagagccgtc aggacgtgct gcaacactgg
      421 ggcttcgaac tgaagcgctg ggtcgatccc gtcacccaga tgccgcgggt aaactacgcg
      481 ctcggtgcca aaggtcctgc tccgcaggac gagcacggcc acaccacatc cggcatcttg
      541 cacaagctta cgagctcggg tccgctgacc actcgcatcc tcaacacggg caagctcaac
      601 tccggtaagc tggacgagca ccagaagtag cgcccgcctc cctcacttcc ctcgcggcgc
      661 gagatctttc tcgaacgtcg tgcggtcgcg cttgagcatg ttcttgtaca tgtcgcgcat
      721 ctcgcggcgc gtgaggcgct tgccgctcgc gaagtattcc agcgcgaccc gaccgatggc
      781 gaacgtgcct atcgccgcga tccccgccga caccgcccac ccggccgtag gcacgagtct
      841 ggcgccctgc tgcgccatgt agcggaacgc cagcccgccc gcgatcgtcc ctatcagctc
      901 gcgcgcgtgg tgcacgttga acggctcgcc gtagatggcc gcgatgcgca gcaccatgcg
      961 cgcctggttc gccagcagca gcggcaggtc gatgaacggg atcggctcgg cgccgatgcc
     1021 cccattgatg atcatcgagt tacgcacgac cttattggcc gccgtgcgcc ggaaggaggg
     1081 taggttgcgc cccagcaaca cggccaggtc cggcagtgcg tcgaccagcg cgggcagcaa
     1141 ccgttcgccg acgttggtgc cctttttggc cgagatggcg atcaccggca cgcccaggcg
     1201 gcgcaccatg tcggtttcga cgctctgcgc gtccttgccc accaggtcga tcttgttggc
     1261 gaccacgacc atgggcttgc ccaggcggcg cagcgcgtcc atcagttgca ggtcactctg
     1321 gcgcagcccg gcggcggcgt cgaacaccac cagcagcgcg tcggcggtgg ccgcgccttg
     1381 cagcgcggtg gccgcgcggt cttcgccccc cgcttcgccg aagccgggcg tgtcgatgag
     1441 cgtgaacggc cccagcccct gctcgatgac cgtgcgcgtg gtgccgggga tagggctagt
     1501 ctgcgaaacc atcttcccgg cgatcaggtt gaacagggtg gacttgcccg aattgaccgg
     1561 gccgacaatc gccagtcgct cctcggcctc gcgctgaacc tcgcgctgca tgccggtcca
     1621 gtttagcgta ttcagcaacg tgcggatgtc gtcgagcgag gtgggtagcc ggggggtcga
     1681 gttggacgaa tcgggcatgc gtggtctcct ctttgcgcgc acggggcgcg gtggattgtg
     1741 aacaggttgt cgatcaacaa cacgaagtaa cacatttacg cacggcgcgg gcgccgggtt
     1801 gctgccgggg tggcgcacgt tacggtcggg cctgctgcct gcaagagatg tgccgttgct
     1861 gctcagggac catcgggcag ggcaggtggg gacctttcgc cctcgtgcgc cgcgcgggca
     1921 ggcgctacaa taaagccgtt aattacgata agcctgtgac gttgggcagg tgttatcgtt
     1981 agggtctgtt atcgggcccc tgcgcgcggg cgcacctctt atccccgcgc cccgcgcagc
     2041 cgatagcccg ctcaacccca cctcttactg ccgccccgcc ttcccgtaag cccgcaaagc
     2101 gcattgccca tcccgccgcc ccggcggtta tggtataatt gacgcggtgt ttgcgtttcc
     2161 gggaccgcaa aggaccctct tcgagacccc ctctgccggg taattgaacg cgcacccgcg
     2221 caatggtgcg cgggcctccg ggcggcttgc ggcagaccgc gggccggcgc cacgggccaa
     2281 tcatcggctt tcttattgta cgctggagga tcgcttgtct tatgtccttg ccgcttgccc
     2341 cggctgctcc gctgctgctt atcggtggcg ggtcggccag ccaacctgtc gatatgttgc
     2401 acgagtttgc caaactggcc ggggggccgg gcgcgcgcat cgccgtgatc accagcgggt
     2461 cgcgggcgcc gcagcaggtc ggggcacgct atgagcggtt ttttcgctcc atcgacatgg
     2521 aggtcggggt ctatcacctg ctgagccgcg cggagtcgaa cgacccagcg gtgctgcagg
     2581 gctttgccga tgcccaggcg tatttcttca ccggcggtga gcagttgtgc atcaccgcgc
     2641 gcatgggcgg gacagaggcg ttgcgcgtgc tgcgccggcg gcacgccgaa ggggcggtgc
     2701 tgggcggcac cagcgcaggc acctcggtga tgagcgagac gatgatcgcc ttcggcaacg
     2761 aggggccggt gcccagccac agcatggtca acctggcgcc gggcctgggc tttctgcacg
     2821 gcatcattct cgatcagcat ttcagccagc gccagcgcct gggccggctc aagacggcgg
     2881 tgtcctacaa ccccgaagaa ctgggcgtcg gtgtggacga ggacacggcg gcgctgttcc
     2941 ggcccgacgg cacgctggag gtgctcggcc ccggcaccgt gaccgtggtg gacgaactcg
     3001 acgtgaccga caacaccagc cccgatgtgg aagatcccga cctgccgctc tgtatcgagg
     3061 gcgtgcgcat ctaccaactg agcgcgggcg agcgctttga ttgcctgggc cgcagcctgg
     3121 ccgccatccg cgcggcggcc tgagtcgggc cggccccagc cgccctgtca ctctgagcgc
     3181 agcgaagagt ctcgtcgccg ccccacacga gacggagatt cttcgctcca ctcagaatga
     3241 cagtttgccg gacggcgtct gagcggtcgg aaacgcccgg attctacccc gcttgtcaag
     3301 cccccatcgg cccggtacaa tgagccccat gaccacccga ctcccgctac gcggcgtgac
     3361 cggcgccgtg ctggcgctga tcttcggcct ctatctgctc accatgtccg ggcacaccta
     3421 ttcgcccgac gaggagacga tgctggcgac cgccgaggca gtggggctgc acggcacctt
     3481 tgccctgccg cgtagccgta ccctggtcga ggtgccgggg ctggacgggc gcttctacag
     3541 tcagtacggg ccggggcaac cggtggcggc ggtcccctgg atctgggccg ggcatggcgt
     3601 ggccgcgctg ctggggctgg acgcggcggg gcagggcttc gtcgtgcgct tcgtgctcgg
     3661 cagctacaac ctgctggtgg ccggcgggtt ggccgcgctg ctctacgccg tggcccagcg
     3721 tttgggcgcc gg
//
LOCUS       DASUYC010000157         4319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1446727, whole genome shotgun sequence.
ACCESSION   DASUYC010000157 DASUYC010000000
VERSION     DASUYC010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446727"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VF276_02495"
     CDS             complement(<1..340)
                     /locus_tag="VF276_02495"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX6605762.1"
                     /translation="MEPQRLLSIDGGGIRGILPLCQLVELERVTGQLTQKSFQFVAGT
                     SVGAIIAAGIAAGIPAARLLDRFISMAGQVFPLRPWNPLVRLVTGHMYSVARLNSVLR
                     GALPEAADLTL"
     gene            complement(385..1113)
                     /locus_tag="VF276_02500"
     CDS             complement(385..1113)
                     /locus_tag="VF276_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605763.1"
                     /translation="MQKLRTTRGLPAVVLLLALAVLAACGGPAAEPTATVPPIVVPTE
                     APTATAAPRDLATATPAPTEAPTQAPTEVPATDTAVPPPPTVTPRPPTAVPPTAAPTS
                     KPKPTRAPVPPTPKPPTGTPVPPTQVAPAGTLAVGTWGGQHIALEVTGAGATYELDCA
                     HGTIDGPIALDAAGHFTAHGTYVQEHGGPTTADETPNSQAVTYTGTVQGDQLSLVITG
                     TASGTDLGKFTLGLGAPGRVLKCL"
     gene            1365..3239
                     /locus_tag="VF276_02505"
     CDS             1365..3239
                     /locus_tag="VF276_02505"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6605764.1"
                     /translation="MDTNPAVASPTGAPAAPADPAPRPAWAARFSAVKTWAIDWARLA
                     PERVLYGCDRTGVAQIYAWDQAADTHTQLTSKPSGVQADLAWLAQDGQTVYYLDDEGG
                     SETGRLVRLPFTGGPPEVVDPSLPLLWVGWVEAPTGGVVLFDGSDATGYRIYRHEGAG
                     PSRVLYEHKNEAYGGPLSHDHGRLAIVTSEQVPNRHWHITVLDAATGARQAELDDGPE
                     ATAVPNAWAPFDGDARLLYTSNVSGRNLPGIWDVAADTRQPLALDLPGEVTGVDWLPD
                     GQGVLLAQHHDGRVQLHTYDLASGTVTALPHSPGTLAPLHVRPDGAIWYNYESAAAVD
                     QVRALEDGQERRLLGGDQALPGTPLESVHFPSTEGASIHGFLGVPPGPGPFPAIVVVH
                     GGPATHATDSYDPFLQADIDEGYAVLTINYRGSSGYGRAHQDIVQGDPGHYELEDMAA
                     ARDYLIARGITEPDRVVVRGRSYGGYLTLMALTRQPALWAAGCALVGIADFAMMYEDA
                     NEPLRGWVRQIFGGGPEDGSLLFMARAPITRAADLRAPLLIIQGRVDTRCPPRQMEVF
                     IARLRELGKPFQVEWFNGGHILVDMAETARLETVFFDFLRGALGDRAGNESPSVAPAG
                     "
     gene            complement(3255..>4319)
                     /locus_tag="VF276_02510"
     CDS             complement(3255..>4319)
                     /locus_tag="VF276_02510"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6605765.1"
                     /translation="LGGIWSTAAAAFLADRLGRRRLLLGFSALMAAAGLAFVALANPW
                     ALSAAALLGTISPTSSETAPFLSLEQAILPQVTPADRRTEVFARYNLVALLAVAGGAL
                     LAGLPGVLAPPGAARLVAERAMFIVYAGLALGAAACFAGLSPAVELHGQDGGTAGRSA
                     PLGPSRGIVLRLAGLFAVDALGGGLAVQSLIALWFHERYGLDLSALGVIFLAANLASA
                     FSLLAAARLARRFGLLNTMVFTHLPSNLLLMAVPLLPGPGLAVAALLARQALSQMDVP
                     TRQAYTMAVVTPAERTAAAGVTSIARSAASATGPSLAGLALQLAPLALGLPFLLAGGL
                     KIVYDLSLYAVFRHIRPGDE"
BASE COUNT          645 a         1596 c         1503 g          575 t
ORIGIN      
        1 tcaacgtcag gtcggcggct tcgggcaagg ccccacgcag cacgctgttg aggcgtgcaa
       61 ccgagtacat gtggccggta accagccgga ccagcggatt ccacggccgc agcggaaaga
      121 cctgtccggc catggagatg aaccggtcca gcagtctcgc ggcgggaatc cccgcggcga
      181 tgccggcggc gatgatcgct cccaccgagg ttcccgcgac aaactggaag gatttttgcg
      241 tgagttggcc ggtcacccgt tccagttcca ccaactggca gagagggaga atcccgcgga
      301 tgccgccccc atcgatgctc aatagacgtt gcggttccat gcgtgctcct tccatccccc
      361 caccatcctc aggtgctgga cgcgctagag gcacttgagc acccgaccgg gcgcgcccag
      421 gccgagggtg aacttgccca ggtccgtgcc gctcgcggtg ccggtgatca ccaggctcaa
      481 ctggtcgccc tgcaccgtgc cggtgtaggt cacggcctgg ctgttgggcg tttcgtcggc
      541 ggtggttggg ccgccgtgct cctggacata ggtgccgtgg gccgtgaagt ggcccgccgc
      601 atccagggca atcggcccgt cgatggtgcc gtgcgcgcaa tcaagctcat acgtggcgcc
      661 cgcgccagtc acttccaggg cgatatgctg cccgccccag gtgccaaccg ccagcgtacc
      721 cgccggggca acctgcgtcg gcggcaccgg cgtgccggtg ggcggcttag gcgtcggcgg
      781 cacgggcgca cgggtcggct tgggcttgct cgtcggcgcc gcggtcggcg gcacggcggt
      841 cggcgggcgg ggagtcacgg tgggcggcgg gggcacggcc gtatccgtcg cgggaacctc
      901 ggtgggcgcc tgggtgggcg cttcggtcgg cgcgggggtg gctgtggcaa gatcccgcgg
      961 cgcggcggtt gcggtgggcg cctcggtcgg caccacaatg ggcggcacgg tggccgtcgg
     1021 ctccgcggcg gggccgccgc aggccgccag cacggcgagt gccagcaaca gaaccaccgc
     1081 cggcaagccg cgcgttgtgc gtaatttctg cataaggcta tccttccttg acctgtgcgc
     1141 gatctatagg atcgtctgga gtcttgacgt ttggcagggc cggcgggttc cgttcccggt
     1201 ccgcccgtgt gcgcgcgccg cgtcgtcgcc gggccgcgga agggtccggc ggcataaccg
     1261 actataccac ggcggtggtc tgtggtacaa tggcaccact gacccgtctc cggcccgctt
     1321 ccaccatgct atcgggccgg aatcccgccc ccagaggaga ttgtatggac acgaaccctg
     1381 ccgttgcctc gcccaccggg gcgcctgccg cccccgccga tcccgcgccc cgccccgcct
     1441 gggccgcgcg cttttccgcc gtcaagacct gggccatcga ttgggcgcgc ctggcgcccg
     1501 aacgcgtatt gtacggctgc gaccgcaccg gcgtcgcgca gatctacgcc tgggaccagg
     1561 ccgccgacac gcacacccag cttaccagca agcccagcgg cgtccaggcc gacctcgcct
     1621 ggctggccca agacgggcag acggtctatt acctggacga cgagggcggc agcgagacgg
     1681 gccgattggt gcggctgccg tttaccggcg ggccccccga ggtggtcgat cccagcctgc
     1741 cgctgctgtg ggtcggctgg gtggaggccc cgaccggcgg ggtggtgctg ttcgacgggt
     1801 cggacgccac gggctatcgg atctatcgcc acgaaggggc cgggccgtcg cgggttcttt
     1861 acgagcataa gaacgaagcc tacggcggcc ccctgtccca cgaccatggc cgcctggcga
     1921 tcgtcaccag cgagcaggtg cccaaccgcc actggcacat caccgtgctc gacgcggcca
     1981 ccggtgcccg ccaggccgag ctggacgacg ggccggaagc gaccgcggtt cccaacgcct
     2041 gggcgccctt cgacggcgac gcccgcctgc tctacaccag caacgtcagc gggcgcaacc
     2101 tgcccggcat ctgggacgtg gccgcggata cccggcagcc gctggcgctc gacctgccgg
     2161 gcgaagtcac cggcgtggac tggctgcccg acggccaggg cgtgctgctg gcacagcacc
     2221 acgacgggcg cgtgcaactc cacacctacg acctggcgag cgggaccgtg acggcgttgc
     2281 cgcacagccc cggcaccctg gccccgctcc acgtccgccc cgacggcgcg atctggtaca
     2341 actacgagag cgccgccgcc gtcgaccagg tgcgcgcgct ggaggacggg caggaacggc
     2401 gcctgctcgg cggcgatcag gccctgcccg gcacgccgct ggaatcggtc cacttcccca
     2461 gcaccgaggg cgcgtccatc cacgggttcc tcggcgtgcc gcccggcccc ggccccttcc
     2521 cggccatcgt tgtcgtccac ggcggcccgg ccacccacgc caccgactcc tacgacccct
     2581 tcttgcaagc cgacatcgac gaaggctacg ccgtgctgac catcaactat cgcggcagca
     2641 gcggctacgg gcgcgcccac caggacatcg tgcagggcga tccgggccac tatgaactgg
     2701 aggacatggc cgcggcccgc gactacctca tcgcccgcgg catcaccgaa cccgaccgcg
     2761 tggtcgtccg cgggcgctcc tacggcggct acctgacgct catggcgctg acccgccagc
     2821 ccgcgctctg ggccgcgggc tgcgcgctcg tcggcatcgc cgacttcgcc atgatgtacg
     2881 aggacgccaa cgagccgctc cgtggctggg tgcgccagat cttcggtggc ggcccggagg
     2941 acggttcgct gctgttcatg gcgcgggcgc cgatcacccg cgccgccgac ctgcgcgccc
     3001 cgctgctcat catccagggc cgcgtggaca cccgctgccc gccgcgccag atggaagtgt
     3061 tcatcgcgcg cctgcgcgaa ttggggaagc cgttccaggt cgagtggttc aacggcggcc
     3121 acatcctggt cgatatggct gagaccgcgc gcctggagac cgtcttcttc gacttcctgc
     3181 gtggagcgct gggcgaccgg gcgggcaatg aaagcccatc cgtcgcgccc gccggctagc
     3241 gcccggaccc gccgctactc gtcgccgggc cggatgtgcc ggaacaccgc gtacaggctc
     3301 aggtcataga cgattttgag gccgccggcc agcaggaagg gcaggccgag cgccagaggc
     3361 gccagttgca gcgcgagccc ggccaggctc ggcccggtgg ccgaggccgc cgagcgagcg
     3421 atcgacgtga cacccgccgc cgccgtccgc tccgccgggg tgacgacggc catggtatag
     3481 gcctgccgcg tgggcacgtc catctgcgag agggcctgcc gggccagcaa ggcggccacc
     3541 gccaaccccg gtcccggcag caggggcacg gccatcagca gcaggttcga cggcaggtgg
     3601 gtgaagacca tcgtgttgag caggccgaag cgccgggcca gccgcgcggc cgccagcagc
     3661 gagaaggccg aggccaggtt ggcggccagg aagatgacgc cgagcgcgct caggtcgagc
     3721 ccgtagcgct cgtggaacca cagcgcgatc aggctttgca ccgctaaccc gccgcccagg
     3781 gcatcgaccg cgaacagccc ggccagcctc agcacaatgc cccgcgacgg ccccagcggc
     3841 gcgctgcgtc ccgccgtccc gccgtcctgg ccgtgcaact ccaccgcggg cgacagcccg
     3901 gcgaagcagg ccgccgcccc gagcgccagc ccggcataca ctatgaacat ggcccgctcc
     3961 gctaccaggc gggccgcgcc ggggggcgcg aggactcccg gcagcccggc cagcagcgcg
     4021 ccgcccgcca ccgccagcaa ggccaccagg ttgtagcgcg caaaaacctc cgtgcgccgg
     4081 tccgcgggcg tgacctgcgg caaaatagcc tgttccagcg acaggaacgg cgccgtctcg
     4141 ctggacgtgg ggctgatcgt gcccagcaag gccgccgcgc taagcgccca cgggttcgcc
     4201 agtgcaacga aggccagccc ggcagccgcc atcagcgccg aaaagccgag caacagccgc
     4261 cgccgcccca gccggtcggc caggaaagcc gccgcggccg tggaccagat accgccgag
//
LOCUS       DASUYC010000158         2231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_328803,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000158 DASUYC010000000
VERSION     DASUYC010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328803"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..310)
                     /locus_tag="VF276_02515"
     CDS             complement(176..310)
                     /locus_tag="VF276_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605766.1"
                     /translation="MLLTIALICFLAQVLVWMMLPASPRPATMPALRESAPREALAVE
                     "
     gene            complement(359..1591)
                     /locus_tag="VF276_02520"
     CDS             complement(359..1591)
                     /locus_tag="VF276_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX6605767.1"
                     /translation="MSTAINGADTAWILIATALVMLMTPALGFFYGGLVRRKNVLSTI
                     MHSFFMLALISVQWVLWGYSLAFGPDKGGLIGGLDWLGLQGVGLAPNPDYAATIPHQA
                     FMIYQAMFAAITPALITGAFAERKRFKAFVIFSLLWATLVYDPVAHWVWGTGGWLRGL
                     GVLDFAGGTVVHITSGVSALVAALMLGRRLGFGQESMDPHDATMTMLGAAMLWFGWFG
                     FNAGSALTSGPLAVNAFVVTNTAAAMAALTWTTVSWMHQQRPSAIGAAAGAVAGLVAI
                     TPAAGFVDARAAIIIGTGAGIVCYWAVQLKHRLKVDDALDVWAVHGVGGWWGAIATGL
                     FATTAVNAAGANGLFFGNPGQVGVQILAVLVSSLYAGGATWVILKLVDRFVGLRVPEA
                     EEIRGLDASQHGEPAYQL"
     gene            2003..>2231
                     /locus_tag="VF276_02525"
     CDS             2003..>2231
                     /locus_tag="VF276_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009227023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6605768.1"
                     /translation="MKSRILVIDDDANITSFLKRALSYEGYTVDTAATGASGLARALQ
                     EPPDLVILDVMMPGLDGYEVATRLRAGGPQPI"
BASE COUNT          457 a          741 c          697 g          336 t
ORIGIN      
        1 acgagacgga gattcttcgc tgcgctcaga atgacaagag ggacatgatc agggagactg
       61 gaaggactat caagcaggac aaaaaggagc caggcgggga actcaaccgg ggagagaccc
      121 tgcctggcac ggaagctatg ggattcgggt caccaggacc cgcggataaa tccgcctatt
      181 cgacggcgag ggcttcgcgc ggggccgact cgcgcaaggc gggcatggtg gccgggcgcg
      241 gcgacgcggg caacatcatc cagaccagca cttgcgccag gaaacagatc aacgcaatcg
      301 tgagcaacat cgtaggaacc tccatgtata cgtgatagga ccgcaattgg atcgcatgct
      361 agagttgata ggccggctcg ccgtgctgcg aggcgtccag gccgcggatc tcttccgcct
      421 ccgggacccg cagtcccacg aagcggtcca cgagtttcaa gatcacccag gtggcgccac
      481 cggcgtacag gctgctcacc agcacggcga ggatctgcac gcccacctgg ccggggttgc
      541 cgaagaagag gccgttggcg cccgccgcgt tcactgcggt cgtggcgaac aggccggtgg
      601 cgatcgcgcc ccaccatcca cccacgccgt gcacggccca gacatcgagc gcgtcgtcca
      661 ccttgagccg gtgcttcaac tgcaccgccc agtagcagac gatgcccgcg ccggtgccga
      721 taatgatggc ggcgcgagca tcgacgaagc ccgccgccgg ggtaatcgcc accaggcccg
      781 cgaccgcgcc cgccgccgcg ccgatggcgc tgggccgctg ctgatgcatc cagctcaccg
      841 tggtccaggt cagcgcggcc atcgccgccg ccgtgttcgt gaccacgaag gcgttcaccg
      901 ccagcggccc cgacgtgagc gcgctgccgg cgttgaagcc gaaccagccg aaccagagca
      961 tggccgcgcc cagcatggtc atcgtggcgt catgcgggtc catcgactcc tggccgaagc
     1021 ccagccgccg tcccagcatc agcgcggcga ccagcgccga cacgcccgac gtgatatgga
     1081 ccacggtacc gcccgcgaag tcgagtacgc ccaggccgcg cagccagccg cccgtgcccc
     1141 agacccagtg cgccaccggg tcgtagacca gcgtggccca gagcaggctg aagatcacga
     1201 aggccttgaa gcgcttgcgc tcggcgaagg ccccggtgat cagcgccggg gtgatcgcgg
     1261 cgaacatggc ctggtagatc atgaaagcct ggtgcgggat cgtggccgcg tagtcggggt
     1321 tgggggccag gcccacgcct tgcagcccca accaatccag tccgccgatc agcccgccct
     1381 tgtcgggccc gaaagccagg ctatagcccc acagcaccca ctgcacgctg atcagcgcca
     1441 gcatgaagaa gctgtgcata atggtggaca ggacgttctt gcggcggacc agtccgccat
     1501 agaaaaaacc gagcgccggg gtcatcagca tcacgagggc cgtggcgatg aggatccagg
     1561 cggtatccgc gccgttaatg gcggtcgaca tagtgcattc ctccaacata agccaggacg
     1621 gggcacccat gccgccggcg agccgcactg ctcaccacca tagcctagcg caaatgcctg
     1681 cccccggcct atggctaggg gaacaaaaag ccaccggcat gttttgtcta tattgcacaa
     1741 aacagataca ccccgaaacg atgaaggcgg ctaaccatcg gcaaaaggat gcgcactggc
     1801 acagcaggcg attcgatctt gtgcactccg cacatattgt gcgcgggggg aaccggcgga
     1861 atccttctta acgattccta ataattctct gcgccttttc ttatgcatat agtagataat
     1921 tatggtgccg catcatgcgt ggcgtgcgcg ggcacgtccg caacttagcg cggcgatgag
     1981 aaaatctgtt atgatagact ccatgaagtc acgcattctc gtcatcgacg atgacgccaa
     2041 tatcacgagc ttcttgaagc gcgccctgtc ctacgagggc tacaccgtgg acaccgctgc
     2101 gaccggcgct tccgggctgg cgcgcgccct gcaagaaccg cccgatctgg tcatcctcga
     2161 cgtgatgatg ccgggcctcg acggttacga ggtcgccacc cgcctgcgcg cgggcggccc
     2221 gcagccgatc c
//
LOCUS       DASUYC010000159         4765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1975993, whole genome shotgun sequence.
ACCESSION   DASUYC010000159 DASUYC010000000
VERSION     DASUYC010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1975993"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="VF276_02530"
     CDS             <1..946
                     /locus_tag="VF276_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tagatose 1,6-diphosphate aldolase"
                     /protein_id="HEX6605769.1"
                     /translation="RNALIQAGLPDPTPAQITEFKAIVTEVLTPHASAILLDPEYSLP
                     VIPKRAPDTGVLLAYEKWGYETSTPGRLPDLLPDWSVRRLVEAGATAIKILLYYDPHD
                     KAEINEVKLAFIERVGTECRGADVPFFLEPIAYSDAIPDEKGRDFAKVKPEKVRKSIE
                     TFSQPRYGVDILKVEVPVNLRYVAGTRAAEGQDPVYDRDEALGYFRQSAAASRLPFIY
                     LSAAVNTPVFHEALEMANEAGVPYSGVLCGRATWQGGIPVYVRDGATAFRRWVETEGV
                     NNIQALNALLAEGAFPWWDAYGGKDRIHLVDSPPGAAV"
     gene            1147..1638
                     /locus_tag="VF276_02535"
     CDS             1147..1638
                     /locus_tag="VF276_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020580042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6605770.1"
                     /translation="MAVAVQIRLLTGEDIDAVVQLSVLAWEPVFESFAQILGPAIYPI
                     VYPDWRRQQAEGVEAVCKDTAKYTVWLAEVGGAVVGFIAYTLSDADKTGEVDLLAVHP
                     DYQNHGIGTDLNTFVLGKMRDAGMRLAVVGTGGDPGHAPARRSYEKAGYTGLPLVRYY
                     KDL"
     gene            complement(1650..3002)
                     /locus_tag="VF276_02540"
     CDS             complement(1650..3002)
                     /locus_tag="VF276_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018754645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HEX6605771.1"
                     /translation="MPVAWQNWSGSVHATPAEVVHPATQDEIIAIVKDCRARGRKLRV
                     VGTGHSFTPLVQTGGVLMVLDRYTGIVSVDAERGQATVRAGIPIQTLGEALFGHGLAQ
                     PNLGDIDVQTIAGAISTGTHGSGGTLGSLSTQVAGLTLITAAGEVLECSEETNRAIFK
                     AAQVSLGALGIISTVTLQLVPAFTLHYVWRKESLRACLAQIDRYVADNRNFEFFWMPY
                     TDAVLAKFMNPTDASPRSKSLVRRFNDSVLENGVFWLMSEYARRFPAQAPAVSRVAGA
                     LMSGGTDINHSHRIFATPRLVKFQEMEYSIPAAEFLPVIEEIDATIRRRRFQVHFPIE
                     CRFVCGDDIPLSPAYGRDSAYIAVHMYKGMAYRPYFAAMEAIFKRHAGRPHWGKLHSR
                     HAGDLPALYPQWDAFQAVRRALDPEGFFLNRHLVALFGEAAPAEARESEAANPAGRPA
                     "
     gene            complement(3137..4324)
                     /locus_tag="VF276_02545"
     CDS             complement(3137..4324)
                     /locus_tag="VF276_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015893747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid deaminase/aldolase"
                     /protein_id="HEX6605772.1"
                     /translation="MSNYETYKAAFQGRQMPFAYVDLDLLDANIRQIVARAGGKPIRV
                     ASKSIRSLPILQHILAADPALRGVLCFTAPEAVYLSRQGLDDLVIGYPIWHPAQIAAV
                     CAQVRQGKTIVAMVDSREHVAHLEAIACAQGVVLPVCLDIDLSTDFPGLHFGVWRSSI
                     FTPRQALAVYRAIAHSPHLRLDGLMGYEAQIAGVGDQVPGQRVRSALIRALKRRSIAQ
                     IAERRARTVALLARHGARLRFVNGGGTGSLESTRAEPCVTEVTAGSGFFAPALFDHYR
                     AFRHAPAAGFAVEIVRKPKPGIYTCSGGGYIASGATGPEKQPQPYLPPGARLLPLEGA
                     GEVQTPVAYQGPVPPALGDPIFLRHSKAGELCERFNTLLLLRQGRVVDEVPTYRGAGL
                     CFL"
     gene            complement(4371..>4765)
                     /locus_tag="VF276_02550"
     CDS             complement(4371..>4765)
                     /locus_tag="VF276_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605773.1"
                     /translation="LQPRAVIESVWVDHNVSENNQTGMRIHVKFTINNLKDSTGQVSA
                     YFYMASGEKVQAKNGYYTTPDGQVAVGSNFVPSYDNALFNDFSFFLPYDALQFGSGSY
                     DLKFHVSIYTPEYRLALSDYKTFTLTVR"
BASE COUNT          814 a         1496 c         1643 g          812 t
ORIGIN      
        1 gcgcaacgcg ctgatccagg ccggcctgcc cgaccccacc ccggcgcaga ttaccgagtt
       61 caaagccatc gtcaccgaag tgctgacgcc tcacgccagc gcgatcctgc tcgaccccga
      121 atacagcctg ccggtgatcc cgaagcgcgc gcccgatacc ggcgtgctgc tggcctacga
      181 aaagtggggc tacgagacga gcacgccggg ccggctgccc gatctgctgc ccgactggtc
      241 ggtgcgccgg ctggtcgagg ccggcgccac ggcgatcaag atcttgctct actacgatcc
      301 ccacgacaaa gccgagatca acgaggtcaa gctggccttc atcgagcggg tgggcaccga
      361 gtgccgtggc gccgacgtgc ccttctttct ggagccgatc gcctacagcg atgccatccc
      421 cgacgagaag ggccgcgact tcgccaaagt gaagccggag aaagtgcgca agtctatcga
      481 gacgttctcc cagccgcgct acggggtgga catcttgaag gtcgaggtgc cggtgaacct
      541 gcgctacgtg gcgggcaccc gtgcggctga ggggcaagac cctgtctatg accgcgacga
      601 ggcgctgggc tacttccgcc agtcggcggc ggcctcgcgg ttgccgttca tctacctcag
      661 tgccgccgtc aacacgccgg tgttccacga agcgctggaa atggcgaacg aggcgggcgt
      721 gccgtactcc ggcgtgctct gcgggcgggc cacctggcag ggcggcatcc cggtctatgt
      781 gcgcgacggc gctaccgcgt tccgccgctg ggtcgagacg gagggcgtga acaacatcca
      841 ggcgttgaac gcgctgctgg ccgagggcgc cttcccctgg tgggacgcct atggcggcaa
      901 ggaccgcatc caccttgtag acagcccgcc gggcgcggcg gtatagcccc ggttgcccgg
      961 cccctgaagg ggcgggtaac ctttgcgaag cccgcctgcg cgggctggtc gaatgagaga
     1021 caggccgcgc aggcgggctt tgcaaggcta gccggaggct tgagcctccg ggcgcgggct
     1081 gatgagtatt agatgtgggt cgcccggccc agcagggggc acgggcggcc ctgctttatg
     1141 cagaggatgg ctgtggcagt acagattcgc ctgctgaccg gcgaggatat tgacgctgtg
     1201 gtgcaactct cggtgctggc gtgggagccg gtgttcgagt cattcgcgca gatcctgggt
     1261 cccgccatct acccgattgt ctatcccgat tggcgccggc agcaagccga gggcgtggag
     1321 gcggtgtgca aggacaccgc gaaatacacc gtctggttgg cggaggtggg cggcgccgtg
     1381 gtcggcttta ttgcttacac cttgagcgac gccgataaaa cgggcgaagt ggatctgctg
     1441 gccgtgcatc cggactatca aaaccacggc atcggcacgg acctgaacac cttcgtgctc
     1501 ggcaagatgc gggacgcggg catgcggctg gccgtggtcg gcaccggcgg cgatccgggg
     1561 cacgcgcccg cccgccggtc ttacgagaag gccgggtata cggggctgcc gctggtgcgc
     1621 tattacaagg atctatagcc gccgttggct caagccggac ggcccgccgg gttggccgcc
     1681 tcggactccc gtgcctccgc gggcgcagct tccccaaata gcgccaccag gtggcggttg
     1741 aggaagaagc cctccgggtc gagcgcgcgg cggacggcct ggaatgcgtc ccactgcggg
     1801 tagagcgcgg gcaagtcccc ggcgtggcgg ctgtgcagct tgccccagtg cgggcgaccc
     1861 gcgtggcgct tgaagatggc ttccatcgcc gcgaaatagg ggcggtaggc catgcctttg
     1921 tacatgtgca cggcgatata ggccgagtcg cgcccgtagg ccgggctgag cgggatgtcg
     1981 tcgccgcaca cgaaccggca ctcgatggga aagtggacct ggaagcgccg gcggcggatc
     2041 gtggcgtcga tctcctcgat caccgggagg aactcggcgg cggggatgct gtattccatc
     2101 tcctgaaact tgaccaggcg cggcgtggcg aagatgcggt ggctgtggtt gatgtcggtg
     2161 ccgccggaca tgagcgcccc ggccacccgg ctcacggccg gcgcctgggc cgggaagcgg
     2221 cgggcgtact cgctcatcag ccagaagacg ccgttttcga gcacgctgtc gttgaagcgg
     2281 cgcaccaggc tcttgctgcg cggcgacgcg tccgtcgggt tcatgaactt cgccagcacg
     2341 gcgtcggtgt agggcatcca gaagaactcg aaattgcggt tatcggccac gtagcggtcg
     2401 atctgcgcca ggcatgcgcg cagcgactcc ttgcgccaga cgtagtgcag ggtgaaggcg
     2461 ggcaccagtt gcagcgtcac cgtgctgatg atgcccagcg cgcccagcga cacctgcgcg
     2521 gctttgaaga tcgcccggtt ggtctcctcc gagcactcca gcacctcgcc cgcggcggtg
     2581 atcagggtca gcccggcgac ctgcgtggac aggctgccca gcgtgccgcc ggagccgtgg
     2641 gtgccggtgc tgatggcccc ggcgatcgtc tgcacgtcga tgtcgcccag gttgggctgg
     2701 gctagcccgt ggccgaatag cgcctcgccc agcgtctgga tcgggatgcc cgcgcggacc
     2761 gtcgcctgtc cccgttcggc atcgaccgac acgatgccgg tgtagcggtc cagcaccatg
     2821 agcacgcccc cggtctgcac gagcggcgtg aacgagtgcc cggtgccgac cacgcgcagc
     2881 ttgcgcccgc gggcgcggca gtccttgacg atggcgataa tctcatcctg cgtggccggg
     2941 tggacgacct ccgcaggcgt cgcgtggacg gagccggacc agttttgcca ggcaacaggc
     3001 atcagtgtac ctccactatc tcttcacact tgtcactctg agcgaagcga agagtctcgt
     3061 ctttcgggct acgagacgga gattcttcgc tgcgctcaga atgacaagaa ggtggctatc
     3121 cggaccgcgt ttctcgctac agaaaacaga gtcccgcgcc gcgataggta gggacctcgt
     3181 cgacgacccg gccctggcgg agcagcagca gcgtgttgaa gcgctcgcag agttcgccgg
     3241 ctttgctgtg gcgcaggaag atggggtcgc cgagggccgg cggcaccggc ccctggtagg
     3301 ccaccggcgt ctgcacctcg cccgcccctt cgagcggcag gagccgggcg ccgggcggca
     3361 ggtagggctg cggttgcttt tccgggccgg tggcgccgga ggcgatgtac ccgccgccgc
     3421 tacaggtgta gatgcccggc ttcggtttgc gcacgatttc gacggcgaac ccggcggcgg
     3481 gcgcatgccg gaacgcgcgg tagtggtcga acagggcggg cgcgaaaaag ccggacccgg
     3541 cggtgacttc ggtgacgcac ggctcggcgc gggtggactc caggctgccc gtgccgccgc
     3601 cgttcacgaa gcgcaggcgc gcgccgtggc gcgccagcag ggccaccgtg cgcgcccgcc
     3661 gctcggcgat ctgggcgatg gagcggcgct tgagtgcccg gatgagggcg ctgcgcaccc
     3721 gctgccccgg cacctggtcg cccaccccgg cgatctgcgc ctcgtagccc atcagcccgt
     3781 cgagccgcaa atgcgggctg tgcgcgatgg cgcgatagac ggccagcgcc tggcgcggcg
     3841 tgaagatgct ggagcgccac acgccgaaat ggaggccggg gaagtcggtg gacaggtcga
     3901 tatccaggca caccggcagc accacaccct gcgcgcaggc gatcgcctcc aggtgggcca
     3961 cgtgctcacg cgagtcgacc atcgccacga tggttttccc ctggcgcacc tgcgcgcaga
     4021 cggcggcgat ctgcgccggg tgccagatgg ggtagccgat caccaggtcg tccaggccct
     4081 gccggctcag gtagacagct tcgggcgcgg taaagcagag cacgccgcgc aatgccggat
     4141 cggcggccag gatatgctgg aggatgggca gggagcggat gcttttggag gcgacgcgga
     4201 tcggcttgcc gcccgcccgc gccacgatct ggcggatgtt ggcgtcgagc aggtccaggt
     4261 ccacataggc gaagggcatc tggcggccct ggaacgcggc cttgtaggtt tcgtagtttg
     4321 acatgctaca ccttagccgt ctagccttga ctatgatcta aacgtataca ctagcgaacc
     4381 gtgagtgtga aagttttata gtctgataaa gctaatcggt attcgggagt gtagatagac
     4441 acatggaatt tgagatcata actgcctgaa ccaaactgta gcgcatcata cggtaggaag
     4501 aaggaaaaat cattaaacaa cgcattgtca tatgaaggta caaagttgga tcccaccgcc
     4561 acctgcccgt ccggtgttgt ataataccca ttcttagctt gcaccttctc gccagaagcc
     4621 atatagaaat aagctgatac ttggcctgtg ctgtctttga gattatttat agtgaatttt
     4681 acatgaattc tcatcccggt ttgattgttc tcgcttacgt tatggtctac ccagaccgat
     4741 tcgataacag cccttggttg aagcc
//
LOCUS       DASUYC010000160         2337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1446536, whole genome shotgun sequence.
ACCESSION   DASUYC010000160 DASUYC010000000
VERSION     DASUYC010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446536"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1255)
                     /locus_tag="VF276_02555"
     CDS             complement(<1..1255)
                     /locus_tag="VF276_02555"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX6605774.1"
                     /translation="MSSIKRSVSVTRLVDIIQGRAYSDPEAATQAAAALIEIGEPAVL
                     PLAQALNEDGPHAHDLLLDTLQKIGEPAVSRLVAILMDPQTRLGLRRDAGFALMALHS
                     KRALEPFLELIGDKRAALRAIAAQGLGELGAQQALDPLLTALRDSHWSVRRGAATALG
                     TLGDPRAVDPLIKTLLDTNRFVRGSAAEALGRLGDALAVESLLVALHDAEPDVRALMA
                     LALGKLGDRRAVPALLALLADEYAESRNSAIWALGALQDRRALEPLVHLLSSENRSSR
                     VDAVQALGKLSDPQAIPSLIKALQDAEPFLRQQAAQVLGKFRDNQAAHALINALSDED
                     AAVRLYAAEALGQIGDGRAVLPLIATLDDTVEDVRAQAAEALADLGDAQALPALRRMQ
                     EQDLGGTYKGSNKDIATAVIRRIQDG"
     gene            complement(1504..>2337)
                     /locus_tag="VF276_02560"
     CDS             complement(1504..>2337)
                     /locus_tag="VF276_02560"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605775.1"
                     /translation="RYTDAALAAFRRLGNRREAAYCLSNLATILSYAPDLREHQRFAQ
                     EALGLFQELDDRRGEALLLLTMAYAAADRRQTAQAEEYGRRALALFRRQGDRRSEAWA
                     LRGIALALAPAGRTPEAGILYSQALDNALSMGDNGILPELYRGQAEVLLALDQPPAAL
                     AAAEKGVASVAPDDGYSLTTTYRALGLARLACGDCPGALDALQQSLAAAKPDTYPLEY
                     ARSCWAQAALLDHAGHLAGAGRRRADALAVVESLVWADSPIPPPADLLTLARHEAREV
                     P"
BASE COUNT          404 a          736 c          788 g          409 t
ORIGIN      
        1 agccgtcctg gatacggcgt ataacggcag tcgcaatgtc tttgttgcta cctttgtagg
       61 ttccacccag gtcctgctcc tgcatccgcc gcaatgcagg tagtgcctgc gcgtcgccaa
      121 gatcggctaa tgcttcggct gcctgtgcgc gcacgtcctc aacggtatca tccagggtgg
      181 caattaacgg cagcaccgca cggccatcgc ctatttgacc aagtgcctca gcagcataca
      241 gccgtactgc cgcatcctca tccgacaatg cgtttatgag cgcgtgggca gcctggttat
      301 ctcgaaattt ccccaagact tgtgccgcct gctgtcgcag aaaaggttcc gcatcctgaa
      361 gggccttaat tagtgaaggg atcgcctgcg gatcggacag cttccccagg gcctggacag
      421 cgtcaacgcg ggaagatcta ttctcactgc tcagtagatg caccaggggt tctagggcgc
      481 gcctatcctg tagcgcgccc agtgcccata tagcgctatt acgcgattca gcgtattcat
      541 cggctagaag cgctagcaac gcgggcaccg cgcggcgatc tccgagttta cccaaagcca
      601 acgccatgag tgcgcgcaca tccggctccg catcgtgcaa cgcaaccagc aatgactcga
      661 ccgctagtgc atcgccaagc cgacccaaag cctcggcggc agaaccccgt acaaaacggt
      721 tggtgtccag tagtgtcttg atgagcggat cgacggcgcg tggatcacct aatgtgccca
      781 aggccgtggc ggccccacga cgtaccgacc agtgggaatc gcggagcgcc gtaagcaggg
      841 gatcaagcgc ctgctgagcg cctagttcac ctaacccctg tgcagcgatt gcacgtaagg
      901 cggcccgttt atctcctatc aattccagga aaggttcgag tgcacgtttg ctgtggagtg
      961 ccatcagggc aaacccggcg tcccggcgca agccaaggcg tgtttgcgga tccatcagaa
     1021 ttgccacaag ccgcgacact gccggttccc cgatcttttg cagcgtgtca agtagaagat
     1081 cgtgtgcatg cggtccatcc tcattaagcg cctgggccag gggtagtacc gccggctcgc
     1141 ctatctcgat gagggcagcc gcggcctgcg tggccgcttc cggatcggaa tatgcgcgcc
     1201 cctgaataat gtccaccagg cgcgttacgg acacgctacg ctttatcgat gacatgggat
     1261 cttccttgct tggctagctg tttttgatca ggtagcggcg aatagcaacg agcatgagta
     1321 ggttgctgta cgacgtaggt caggtgtaag ccggatgtgt gtgtgtccag gggcgcagcg
     1381 gcattctcca agaatcatcc ttcagcactg tcgggtgccc gcgcccgtac ccaggatggg
     1441 gtggaccaag gcggggtgga aatgggcgag gtcgccggtg gaggcgtagc cccccgcgag
     1501 gccctacggc acctctcgcg cctcgtggcg ggccagggtg agcaggtcgg cgggcggggg
     1561 gatcgggctg tcggcccaga ccagcgactc gaccacggcc agggcatcgg cccggcggcg
     1621 gcccgcgccg gccaggtggc ccgcgtggtc cagcagggcc gcctgcgccc agcaactgcg
     1681 ggcgtactcc agcggatagg tgtcgggctt agcggcggcc aggctctgtt gcagggcgtc
     1741 gagcgcgccg ggacagtcgc cgcaggccag ccgggccaga cccagggcgc ggtaggtggt
     1801 ggtcagcgag tagccgtcgt cgggcgcgac gctggcgacg cccttctcgg cggcggccag
     1861 ggcggcgggc ggctggtcga gcgccagcaa cacctcggcc tggccgcggt aaagttcggg
     1921 caggatgccg ttgtcgccca tggacagcgc gttatcgagc gcctggctgt aaaggatgcc
     1981 ggcctccggc gtgcgcccgg cgggggccag ggccagcgca atgccgcgca acgcccaggc
     2041 ttcgctgcgc cggtcgccct gccggcggaa gagagccagc gcgcgccgcc cgtattcctc
     2101 ggcctgggcg gtctggcgcc ggtcggcggc ggcgtaggcc atggtcagca gcagcagcgc
     2161 ctcgccgcgg cggtcgtcca gttcctggaa caggcccaac gcttcctggg cgaagcgctg
     2221 gtgctcgcgc aggtcggggg cgtaactgag gatcgtcgcc aggttgctca agcagtaggc
     2281 ggcctcgcgg cggttgccca ggcgacggaa ggcggccagg gccgcgtcgg tgtagcg
//
LOCUS       DASUYC010000161         2564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1689389, whole genome shotgun sequence.
ACCESSION   DASUYC010000161 DASUYC010000000
VERSION     DASUYC010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1689389"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="VF276_02565"
     CDS             complement(<1..670)
                     /locus_tag="VF276_02565"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6605776.1"
                     /translation="MSEPMPRPPAGSLLATKLFVPPPASAQVSRPRLLARLDEGRRQG
                     RRLTLVIAPPGWGKTTLLSAWLGSLAGDPAGRAPAVAWVSLDAGDNDPLRFWTYVCAA
                     LRAAYPDLGGLLPLLQAPQPPPLESLLTALLNAVAAPPAPPALLVLDDYHVIDAPAIH
                     QALAFLIEHLPPHLHLVLAGRADPPLPVGRLRARDALTELRAADLGFTPTEAAAFLTA
                     TMGLR"
     gene            complement(927..2084)
                     /locus_tag="VF276_02570"
     CDS             complement(927..2084)
                     /locus_tag="VF276_02570"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX6605777.1"
                     /translation="MQIQVVPDTLTRLAQLGDVTAFEPAGDQPAQEAPARRYQSHSLA
                     ECITNVTTPGGRKPILKAMLTTACERNCFYCPFRAGRSKTARVTFTPDEMARGFLALQ
                     QAGQADGVFLTSGIIRGSTTTQDRLIATAEMIRRAGYQGYLHLKIMPGIEYDQLHRAM
                     QLATRVSVNLEAPTQERLNALAPKKDWGRELLQMLEWTAAIRRAHPEERLAGTVTQFV
                     VGAAGDTDRELLGLSARLYRDLRLTRTYFSAFHPIAQTPLENLPATDPLREHRLYQAG
                     FLLRDYGWGVDDLPFRDDGNLRTDVDPKRAWGDAHLRHTPLEIMTAARADLLRVPGLG
                     AVAADAILRARRQVRLTDLAQLRRLGIRTADQAAPYILLDGRRPAAQLRLF"
     gene            2383..>2564
                     /locus_tag="VF276_02575"
     CDS             2383..>2564
                     /locus_tag="VF276_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605778.1"
                     /translation="MKWRVFAVTASLTALAGGAALAGALGATPAAAGTLAAPQAQTAQ
                     ATPTPGAQATPPSTPGT"
BASE COUNT          383 a          794 c          989 g          398 t
ORIGIN      
        1 gccgcagccc catcgtggcg gtgaggaagg ccgcggcctc cgtgggcgta aagcccaggt
       61 cggcggcgcg caattcggtc agcgcgtcgc gtgcccgcag gcggcccacc ggcaacggcg
      121 ggtcggcgcg cccggccagc accaggtgca gatgcggcgg cagatgctcg atcagaaagg
      181 ccagcgcctg gtggattgcc ggagcgtcga tcacgtggta gtcgtcgagt accagcaggg
      241 ccgggggcgc gggcggggcg gccaccgcgt tgagcagggc cgttagcagc gactccaggg
      301 gcggtggctg cggcgcctgg agcagcggca gcaggccccc gagatccggg taggcggcgc
      361 gcagggcggc gcagacatag gtccagaagc gcagcgggtc attatcgccg gcgtcgagcg
      421 acacccaggc gacggcgggg gcgcggcccg cgggatctcc ggcgaggcta cccagccagg
      481 cgctgagcag cgtggttttg ccccagccgg gcggcgcgat aaccagggtg agccgccgcc
      541 cctgccgccg cccttcatcc agccgggcga gcaggcgcgg gcgcgacacc tgggccgagg
      601 cgggcggcgg gacgaacagc ttggtcgcca gcaggctccc ggcgggcggg cggggcatgg
      661 gttcagacat gggcggcaac agcggcggca cccctatggc gccgccggct agtggtgtcc
      721 tctcttgggt cgaggtccgc gtcgcgtgat caggctcact atagcaggcc gcctgggact
      781 ctgcaagcgg cccagcctcg cattcgttcc caagttcacc gtgaagaaac gaaggacgcg
      841 aagacgattt taattagttt tgttcttcgc gctcttcgcg tcttcgcggt gcagaacatg
      901 ggtgtgggaa ggagtaggag gcggggctag aagaggcgga gctgggcggc ggggcggcgc
      961 ccgtcgagca ggatgtaggg ggcggcctgg tcggcggtgc ggatgccgag gcggcgcagt
     1021 tgggccaggt cggtgaggcg gacctggcgg cgggcgcgca ggatcgcgtc ggcggcgacc
     1081 gcgccgaggc cgggcacgcg cagcaagtcg gcgcgggcgg cggtcatgat ttcgagcggc
     1141 gtgtggcgca ggtgggcgtc gccccacgcg cgcttggggt ccacgtcggt gcgcaggttg
     1201 ccgtcgtcgc ggaagggcag gtcgtccacg ccccagccgt agtcgcgcag cagaaagccg
     1261 gcctggtaaa gccggtgctc ccgtagcggg tcggtggcgg gcaggttctc cagcggggtc
     1321 tgggcgatgg ggtggaaggc cgagaagtac gtgcgcgtca ggcgcaggtc gcggtagagg
     1381 cgcgcgctga ggcccaacaa ctcgcggtcg gtgtcgcccg ccgcgccgac gacgaactgg
     1441 gtgacggtgc ccgcgagccg ctcttcgggg tgcgcccggc ggatggcggc ggtccattcg
     1501 agcatctgca acaattcgcg gccccagtct ttcttgggcg ccagggcgtt cagccgttcc
     1561 tgtgtgggcg cctccaggtt gaccgagacg cgcgtggcaa gctgcatggc acggtgcaac
     1621 tggtcgtact cgatgcccgg cataatcttc aggtgcaggt agccctggta gcccgcccgg
     1681 cggatcatct cggcggtggc gatcaggcga tcctgcgtgg tggtgctgcc gcggatgatt
     1741 ccggaggtga ggaacacgcc gtcggcctgc ccggcctgtt gcagcgccag gaagccgcgg
     1801 gccatctcgt cgggggtgaa ggtgacgcgg gcggttttgc tgcgcccggc gcggaagggg
     1861 cagtagaagc agttgcgctc gcaggccgtg gtcagcatcg ccttgaggat cggcttgcgc
     1921 ccgcccggcg tggtcacgtt ggtgatgcat tcggccaggc tgtgcgactg gtagcggcgc
     1981 gcgggggcct cctgggccgg ctggtcgccg gccggctcga aggccgtgac atcgcccagt
     2041 tgcgccaatc gggtcagcgt atcgggcacg acctggatct gcataggccc attgtagcga
     2101 acatctgttc ggctgtcaat agatacgctt tgaccatcaa cgtcaccgcg aagacgcgaa
     2161 gcgcgcgaag aatttttaga ctgggtctac tcatcgcgtt ttcgtggtgc gattaactgg
     2221 gtagacccgg cgcgccgcta tctcacccaa acctaatcca atcctaactt ttcgccagcc
     2281 gtcgtctaat tttgcccggc tacactaggg atgtgctgca cacgggtgca gcgtcgtgaa
     2341 tccatgttcg tggtgcgtgg caccagcaaa ggagcttgag ttatgaagtg gagagtattc
     2401 gccgtcactg cgtcgttgac ggcgctggcg ggtggtgcgg cactggccgg tgccctgggg
     2461 gcaaccccgg cggccgccgg cacgctggcc gcgccccagg cgcagacggc ccaggcgacg
     2521 ccgactcccg gtgcgcaagc gacgcccccc agcacaccgg gcac
//
LOCUS       DASUYC010000162         5218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_800213,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000162 DASUYC010000000
VERSION     DASUYC010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5218
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800213"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="VF276_02580"
     CDS             complement(<1..909)
                     /locus_tag="VF276_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX6605779.1"
                     /translation="MKRIFQAIACLTVAMLVLAGCGNETAPTATPVSSAPAGGQGSAP
                     AQSGERSAIHIAVVTHGQASDPFWSVVKKGVDQAGKDMGVTVTYQAPDKFDMVAMSQL
                     IDAAVATKPDGLVVSIPDANALSPSIKKAIAAGIPVISINSGSDVAKQLGVLVHVGQT
                     EYEAGLGGGEKMAAAGVKYALCVNQEVGNAGLDARCKGFTDAMTKAGAKVEVLAVDLA
                     NPTESQQRIQAALQSKPDVDGILTLGPTGATPALAALKAGNMMSKIKLATFDLSPDVL
                     QAINSGDMLFAIDQQQYLQGYLPIVLL"
     gene            complement(1002..2885)
                     /gene="iolD"
                     /locus_tag="VF276_02585"
     CDS             complement(1002..2885)
                     /gene="iolD"
                     /locus_tag="VF276_02585"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HEX6605780.1"
                     /translation="MTTETTERMTMAQALIAFLKNQYVERDGREQPFFAGCFGIFGHG
                     NVAGVGQALQQMPDFRYYQARNEQAMVHAAVAYAKTRNRLQTLACTTSIGPGATNMIT
                     GAATATVNRLPVLLLPGDIFARRNVAPVLQQIEWEHSQDASANDCFKPVSRYWDRLNR
                     PDQLLTALPEAMRVLTSPAATGAVTLALPQDVQTEAYPYPAAFFRKRIWHIPRNRPDR
                     AALREAAAWIRQSARPLIVAGGGVIYSEATEALRRFIEATGIPSGETMAGKGSLPYDH
                     PLNMGAVGVTGTLAANRLAHGADLVIGIGTRYSDFTTASKTAFQDPGVRFVNINVAEF
                     DAAKHLALALVGDAQVTLEELAAELAGYHVAAEYEAAARALHDEWEHEVDRIYAIRNT
                     PLPSQGELIGAVNELSSPAAIMVCAAGSLPGDLHKLWRARHPKNYHLEYGYSCMGYEI
                     AGGLGIKMAAPDRDVYVMVGDGSYLMMNSEIVTSVQEGYKLIIVLLDNAGFKSIGSLS
                     RSLGQQGFGTRYVYPQNGVLPGDEAADVQTLPVDLAANARSLGAQVITCTTYDDFTAA
                     LGAAQQTDRTTVIYIRNDRYVGVPGYESWWDVAVAEVSDLPTVQAARKEWEALRARER
                     EYF"
     gene            complement(2890..3963)
                     /locus_tag="VF276_02590"
     CDS             complement(2890..3963)
                     /locus_tag="VF276_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015131851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX6605781.1"
                     /translation="MAQRSVSIEDIARAAGVSHSTVSRALRNSPLISPDVRTRIQHLA
                     GEMGYTPNALAQSLQTHRSHTLGLVVTSIADPFIADVVRGVEEIARPAGFSVFLSAAY
                     NDPAQEVEVIETFHRRRVDGILVASGRISSNYEAHLSRVKVPTVLLNNEARGDGRRLH
                     WVRVDDDLGAQLATDHLLQLGHRAIGYLGVANRPRSNDQRLAGYRAALAAARVAAPDA
                     WVALPDAAGDEAQDGPAADLAAGQRLLPQLLAAGVTAVFCFNDMVAVGALLACRARGL
                     VVPRDLSIVGYDDIEVARYVTPPLTTVAQPRLRLGTLATQVLLDLLAERPVQNHVLPP
                     ALAIRESTAPPCPASIPARPIGD"
     gene            complement(4319..4816)
                     /locus_tag="VF276_02595"
     CDS             complement(4319..4816)
                     /locus_tag="VF276_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX6605782.1"
                     /translation="MQARMNYSKVAPGAVKAIQGLEAYNHTSSIEPELRELVKLRASQ
                     LNGCAYCVDMHSLDARALGETEQRLYALPVWQDTPFFSERERAALLWAEKLTLLAVDH
                     VPDEVYEQVRPHFTDEELADLTMVISAINVWNRFSVAFRAVPGHYRPAPQPESAPVHE
                     ASPVQ"
     gene            5038..>5218
                     /locus_tag="VF276_02600"
     CDS             5038..>5218
                     /locus_tag="VF276_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEX6605783.1"
                     /translation="MSIDILITGARVVDGTGNPWWYGDVAIQGDRIAAVTPPGLIAPD
                     QAGEVVDAQGMVVCPG"
BASE COUNT          792 a         1669 c         1828 g          929 t
ORIGIN      
        1 cagcagaacg atgggcaggt agccttgcag atattgctgc tggtcgatgg cgaacagcat
       61 atcaccgctg ttgatcgcct gcaacacgtc gggcgagagg tcgaaggtgg cgagcttgat
      121 cttgctcatc atgttgcccg ctttgagggc ggccagggcc ggcgtggccc ctgtcgggcc
      181 gagggtcagg atgccgtcca cgtcgggctt cgattgcagg gcggcctgga tgcgctgctg
      241 cgactcggtg gggttcgcca ggtccaccgc cagcacttcg actttggcgc cggccttggt
      301 catggcgtcg gtgaagccct tgcagcgggc gtcgagcccc gcgttgccca cttcctggtt
      361 cacgcacagg gcgtacttga cccccgccgc ggccatcttc tcgccgccgc ccagcccggc
      421 ttcatattcg gtctggccga cgtgcaccag cacgcccagt tgcttggcga catcgctgcc
      481 ggagttgatc gagatcaccg ggatgccggc ggcgatagcc ttcttgatcg aggggctaag
      541 ggcgttcgcg tcggggatcg agaccaccag cccgtcgggt ttggtggcga ccgccgcgtc
      601 gatcagttgg gacatggcga ccatgtcgaa cttgtcgggc gcctggtagg tcacggtcac
      661 gcccatgtct ttcccggcct gatccacgcc tttcttcacc accgaccaga aggggtcgga
      721 tgcctggccg tgcgtcacca cggcgatatg gatggcggag cgttcgccgc tctgggccgg
      781 ggccgatccc tgccccccgg cgggcgcgct gctaacgggg gtggcggtcg gcgcggtctc
      841 gttgccgcag ccggccagca cgagcatggc aaccgtgaga caagcaatcg cctggaatat
      901 gcgtttcata gtctcctcct ttgggggctg ttgctctggt gcgtctagcc gagtccgtta
      961 cctcgtcaag ggccgctcgt gataggcatc ctcctccttt cctagaaata ctcgcgctcg
     1021 cgggcgcgca gcgcttccca ctccttgcgg gcagcctgga cggtgggcag gtcgctcact
     1081 tcggccaccg ccacgtccca ccagctttcg tagccgggca cgccgacgta gcggtcgttg
     1141 cggatataga tcaccgtggt gcggtcggtc tgctgggcgg cgccgagggc ggcggtgaag
     1201 tcgtcataag tcgtgcaggt gatcacctgg gcgccgaggc tgcgcgcgtt ggcggccagg
     1261 tccacgggca gggtttgcac atccgcggcc tcgtcgccgg gcaggacgcc gttctgcggg
     1321 tagacataac gtgtgccgaa gccctgctgg ccgagcgacc ggctgagcga gccgatgctc
     1381 ttgaagccgg cgttgtccag cagcacgatg atcagcttat agccttcctg caccgaggtg
     1441 acgatctcgc tgttcatcat caggtacgag ccgtcgccca ccatgacgta cacatcgcgg
     1501 tcgggcgcgg ccatcttgat gcccaggccc ccggcgatct cgtagcccat gcagctatag
     1561 ccgtattcca ggtggtagtt cttggggtgg cgcgcccgcc agagtttgtg caggtcgccc
     1621 ggcaagctgc ccgccgcgca gaccatgatc gcagcggggc tgctgagttc gttcaccgcg
     1681 ccgatcagct cgccctggct gggcagcggc gtgttgcgga tagcatagat gcggtcgact
     1741 tcgtgttccc actcgtcgtg tagagcgcgg gccgccgctt cgtactccgc cgcgacatgg
     1801 tagccggcca gttcggcggc cagttcctcc agcgtgacct gggcatcgcc caccagcgcc
     1861 agcgccaggt gcttggccgc gtcgaactca gcgacgttga tgttgacgaa gcgcacgccg
     1921 gggtcctgga aggcggtttt ggacgccgtg gtgaagtcgc tgtagcgcgt gccgatgccg
     1981 atgaccagat cggcgccgtg cgcgaggcgg ttggcggcga gagtgccggt cacgccgacg
     2041 gcgcccatgt tcagcgggtg gtcgtagggc aggctgccct tgccggccat cgtctcgccg
     2101 gacgggatgc cggtagcttc gataaaccgg cgcaacgcct cggtggcctc cgaatagatc
     2161 accccgccgc ccgctacgat cagcgggcgc gcgctctgcc ggatccacgc cgcggcttcc
     2221 cgcagtgccg ccctgtcagg ccggttgcgc gggatgtgcc agatccgctt gcggaagaac
     2281 gccgccgggt aggggtacgc ctcggtctgc acatcctgcg gcagggccag cgtgaccgcg
     2341 ccggtcgcgg cggggctggt cagcacgcgc atggcctccg gcagggcggt cagcaactgg
     2401 tcggggcggt taaggcggtc ccagtagcgg ctcaccggct tgaagcagtc gttggccgag
     2461 gcgtcctggc tgtgttccca ctcgatctgc tggagcaccg gggcgacgtt gcgccgggca
     2521 aagatgtcgc ccggcagcag cagcaccggc aggcggttga ccgtggccgt ggccgcgccc
     2581 gtgatcatgt tggtggcgcc cggcccgatc gacgtggtgc aggccagggt ttgcagccgg
     2641 ttcctggtct tggcgtaggc cacggcggca tggaccatgg cctgctcgtt gcgggcctga
     2701 tagtagcgga agtcgggcat ctgttgcagc gcctggccca cgccggccac attgccgtgg
     2761 ccgaagatgc cgaagcaccc ggcgaagaag ggttgctccc ggccgtcgcg ttcgacatac
     2821 tggtttttga gaaaggcgat cagcgcctgg gccatcgtca tccgttcggt cgtctctgtg
     2881 gtcatcgggc tagtctccta tgggccgggc ggggatggac gccgggcacg gcggggcggt
     2941 gctctcgcgg atcgccagcg ccggcggtag cacatggttc tggaccgggc gctcggccag
     3001 caggtccagc agcacctggg tcgccagggt gcccaggcgc aggcgcggct gggccaccgt
     3061 cgtcagcggg ggcgtgacgt agcgggccac ttcgatgtcg tcgtagccga cgatgctcag
     3121 atcgcgcggg accaccaacc cgcgtgcccg gcaggccagc agtgccccaa ccgccaccat
     3181 gtcgttgaag cagaacacgg cggtaacccc cgcggccagc aactgcggca ggagccgctg
     3241 cccggcggcg aggtcggctg ccggcccatc ctgggcctcg tcgcccgcgg cgtcgggcag
     3301 cgccacccag gcatcgggcg cggcgacgcg ggcggcggcc agcgcggccc ggtagccggc
     3361 caggcgctgg tcattcgagc gcgggcggtt cgccacgccg agatagccga tggcgcggtg
     3421 gcccagttgc agcaggtgat cggtggccag ttgcgccccc aggtcgtcgt cgacgcgcac
     3481 ccagtggagc cgccggccgt cgccgcgcgc ctcgttgttg agcagcaccg tcggcacttt
     3541 gacccggctc aggtgcgctt cgtagttgct gctgatgcgc ccggaggcga cgaggatgcc
     3601 gtccacgcgc cgccggtgga aggtctcgat cacctcgacc tcctgggccg ggtcgttgta
     3661 cgcggcgctg aggaacacgc tgaacccggc gggccgggcg atctcctcga ccccgcgcac
     3721 cacgtcggcg ataaagggat cggcgatgga ggtcacgacg aggccgagcg tgtgcgaacg
     3781 gtgggtttgc agactctggg cgagcgcgtt gggcgtgtag cccatctccc cggccaggtg
     3841 ctggatgcgc gtccgcacat cggggctgat cagcgggctg ttgcgcagcg cccgcgacac
     3901 ggtggagtgc gacacccccg cggcgcgtgc aatatcctca atcgacaccg aacgctgcgc
     3961 caaaatgcga tcctccgcga gaattggctc ctcggctgcc actaccatgc agatggtgcg
     4021 ggcgtgctgc gggacgctgg acgaggaaga cgcgatgctg atccgggtgc gctgaactga
     4081 tcgggatagg gtcatgcaca cgtgtgcatg ggcagagtat ataccaacct cagcgccctg
     4141 tcaataggtt ggctgccggt atttggggtc gaaaccggaa cgcggacctg tacgtgcagt
     4201 gcgctgcacc gtagcaggat aggtgtcgcc gcccggcacg ggcaatacgc gaatggcgtg
     4261 gaaagcgcgg cggcagcagg caacgctgct gccgccggat ggggctaccg ttcaggatct
     4321 actgcacggg gcttgcttca tggacaggcg cgctttccgg ctgcggggcg gggcggtagt
     4381 ggccggggac cgcgcggaag gcgacgctga agcgattcca gacgttgatg gccgaaatca
     4441 ccatcgtcag gtccgccagt tcttcgtcgg taaaatgggg ccggacctgc tcgtagacct
     4501 catcgggcac atgatcgacg gcgagcaggg tgagcttttc ggcccagagc agggcagcgc
     4561 gttcgcgttc gctgaaaaac ggcgtatctt gccacaccgg caaggcatac aggcgctgct
     4621 cggtttcgcc cagggcacgg gcatccaggc tgtgcatgtc cacgcaatac gcacagccgt
     4681 tgagctgcga ggcgcgcaat ttcaccaact cgcgcaattc cggctcgatg ctggaggtgt
     4741 ggttgtaggc ttcgagtccc tggatcgctt tcacggcgcc cggcgccact ttcgagtagt
     4801 tcattctggc ttgcattgct atccatcctt tgcatttgta ttccgggagc ggctattgct
     4861 ccaccactat cataccacta taatgcagtt atcaaaaggg ccaattatgc gccaacgaag
     4921 gtagccatta agaaagattc ggaatccggc gctggtccag gaagacgccg agggcttatc
     4981 tctacggacc ggcccgcggc agcgatcctc atctgttgtt agcggaagga gcctggtatg
     5041 agcatcgata ttctgatcac cggcgcccgc gtcgtggacg gcaccggcaa cccctggtgg
     5101 tatggcgacg tggcgatcca gggcgaccgc atcgccgccg tcacgccgcc cggcctgatc
     5161 gcgccggacc aggcgggcga ggtggtggac gctcagggca tggtcgtctg ccccggtt
//
LOCUS       DASUYC010000163         2234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1257434, whole genome shotgun sequence.
ACCESSION   DASUYC010000163 DASUYC010000000
VERSION     DASUYC010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1257434"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            310..1185
                     /locus_tag="VF276_02605"
     CDS             310..1185
                     /locus_tag="VF276_02605"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6605784.1"
                     /translation="MPPRATQWTPALWVQQSRLYHTNSGIVIHRGRACLIDPGIFPAE
                     IVALARLVAAQGATVAAIILTHSHWDHLLGPEHFPGVPVVAHAAYPATVVAHGAEQMR
                     AVGAWAARHRIKRKHPFRLPQPTTAVATPATYTLGDLTLRLDHAPGHWPDQITLYDPA
                     SGVLWTADMLSDREIPFVSHSLGAYRETLARLAELDIGVLVPGHGQTTRDRAEIAARL
                     AADSTYLDELQTRVRAAVAGGLSLAETVAACARMPYRHRAANAGPHRWNVASAYRELG
                     GAVGAEPVGWDLENL"
     gene            complement(1191..>2234)
                     /locus_tag="VF276_02610"
     CDS             complement(1191..>2234)
                     /locus_tag="VF276_02610"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEX6605785.1"
                     /translation="YSDSNKDGGYLAATWQTYGAQAALAYAAASVGVDLLIFHGRGGA
                     IGRGGGPMNRAILARPPIARYPNLKVTEQGEVISARYGHPVIAERHFEQVIHALLVSA
                     FGPVEDSPDPDWIATMDRLADRSRARYEELVKETPDFLAFFREATPFPELGTLNLASR
                     PVSRAAQSTGDLTLDDLRAIPWVFSWSQVRVNLPGWFGLGTALQEEIAAGGLPQLQSM
                     YRNWRFFATALDNAQLSLGIADMPAARRYASLATRGAELFDQIVAEYEASVAAVLAVA
                     RQSELLERSPVLSRSIKLRNPYVDALHIAQIALLRRYRTLPPDASEEERAALLDAIHH
                     SINGIAAGLQTTG"
BASE COUNT          334 a          798 c          780 g          322 t
ORIGIN      
        1 ggcgtgggcg tctcgcccgg cgccaacctc gatgcgccca gttcgctgag cgcgggtccc
       61 gccggccggg tggtcgaagc gccgcagcag gcccatccgt gagccgttcg cgctaggttc
      121 caccggcgcg gcaggggcgg cgccggccgg acagcacttt gctccgggct gagtagggag
      181 acggctcgcg gcagagtgct gccccgccgg cgtcgccctt gccgcgttct cgcgcgagtg
      241 gcgcgccggg ctggatgtcg ggtatagtgg cggcgcgacg acctgaccga aaggaaccga
      301 gaacccgcca tgccgccccg cgccacgcaa tggaccccgg cgctctgggt gcagcagagc
      361 cgcctatacc ataccaacag cgggatcgtg atccaccggg gccgcgcctg cctgatcgat
      421 cccggcatct tcccagcgga gatcgtggcc ctcgcccggc tggtggccgc gcagggcgcc
      481 acggtcgcgg cgattatcct cacccacagt cattgggacc acctcctggg gccggaacac
      541 tttcccggcg tgccggtggt cgcccacgcc gcctacccgg ccaccgtagt ggcccacggc
      601 gccgagcaga tgcgcgccgt cggggcctgg gcagcgcgcc accgcatcaa gcgcaagcac
      661 ccttttcgcc tcccccagcc caccaccgcc gttgccactc ccgcaaccta tacgctcggc
      721 gatctgaccc tgcgtctcga ccatgcgccc ggccactggc ccgaccagat cacgctttac
      781 gatccggcga gcggcgtgct gtggacggcc gatatgctca gcgaccggga aattcccttt
      841 gtcagccaca gcctgggcgc ctaccgggag acgctggccc ggctggcaga gctcgacatc
      901 ggggtgctgg tgccggggca cggccaaacc accagggacc gcgcggagat cgcggcgcgg
      961 ctggccgcgg attccaccta cctggacgag ttgcaaacgc gggtccgcgc cgccgtggcc
     1021 ggcggcctgt ccctcgccga gaccgtggcc gcgtgcgccc ggatgcccta ccgccaccgg
     1081 gcggcgaacg ccgggccgca ccgctggaac gtcgccagcg cctaccgcga actgggcggc
     1141 gccgtgggcg cggagccggt tggctgggat ctggaaaacc tgtaacgggt ctacccggtg
     1201 gtttgcaagc cggcggcgat gccgttgatg ctgtggtgga tcgcgtcgag cagggcggcc
     1261 cgttcctcct ccgaggcgtc cggcggcaaa gtgcggtagc gacgcaacaa ggcgatctgg
     1321 gcgatgtgca gggcatcgac gtagggattg cgcagcttga tcgaccggga cagcaccggc
     1381 gagcgttcga gcagttcgct ctgccgggcc acggccagca cggcggccac gctggcctcg
     1441 tattcggcca cgatctggtc gaaaagctcc gcgccccgtg tcgccaggga tgcatagcgc
     1501 cgggccgccg gcatatcggc gatgccgagg ctgagctggg cgttgtcgag tgcggtggca
     1561 aagaagcgcc agttgcgata catcgattgc aattgcggca gcccacccgc cgcgatctcc
     1621 tcctggagcg ccgtgcccag cccaaaccag cccggcaggt tgacccgcac ctgcgaccag
     1681 ctaaacaccc acgggatggc gcgcaagtcg tccagagtca ggtcgccggt gctctgcgcg
     1741 gcgcggctca ccgggcgcga ggccaggttg agtgtgccca gttcggggaa aggcgtcgcc
     1801 tcgcggaaga aggcgaggaa atcgggcgtt tctttgacca gttcttcata gcgcgcgcgc
     1861 gaccggtcgg ccagccggtc catcgtggcg atccagtcgg ggtccggcga atcctccacc
     1921 ggcccaaacg ccgagaccag cagcgcatgg atcacctgct cgaaatggcg ctcggcgatg
     1981 accgggtgac cgtagcgggc ggagatgact tcgccctgct cggtcacttt caggttgggg
     2041 tagcgggcga tgggcgggcg ggccaggatg gcgcggttca tcggcccgcc gccccgcccg
     2101 atcgccccgc cccgcccatg gaagatcagc aggtccacgc ccaccgacgc cgccgcatag
     2161 gccagggccg cctgcgcgcc gtaggtctgc cacgtcgcgg ccaggtaccc gccgtccttg
     2221 ttgctgtcgg agta
//
LOCUS       DASUYC010000164         2786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_980519,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000164 DASUYC010000000
VERSION     DASUYC010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980519"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2786
                     /locus_tag="VF276_02615"
     CDS             <1..>2786
                     /locus_tag="VF276_02615"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEX6605786.1"
                     /translation="VIQDPQTALYPSMPLALPPTVVDYTVDVEEMGPLLYELTQGLAQ
                     PAPPPELADPLHDIMDRVSQQSSIDFRQYKPATIVRRIFRRMTVTHTATMRDYVRYLD
                     QHPEEVGELVRSFLIKVTEFFRDRDAFVYLKNTILPDLITRARAKERVLRFWSAGCAT
                     GEEPYSLALLLADLLGPELGEWSVRIFATDLDTDAVAFARRGVYPENLLRNLPDDYRQ
                     RFFEPTDRGFRVAKFLRQMVIFGEQDLSRGVPFPRIDLVICRNLLIYFKPELQQTVLD
                     IFAYSLQQANGYLFLGQAETVRPSKAIYELVNKRWKIYRCLSGPLPLLNRPVARLPAT
                     LPPDRPSGPRRPPVELTELRSPPPPVSEVELGQLRRLNEWVLRFLPHGVVVIDRTYRI
                     LTINGTARRLLGIREAGVDQDFLHSVRGLPYEEVRGAIDTVFRERGGVTLPELEVEAT
                     ASGELRYLTLSIVLMHLDNDGPDLALISVTDLTEQVQTRRHLESVQRDQKQLLEELQV
                     TNRRFGEMNKELQDANEELQAANEEMMLTQEELQATNEEFEATNEELQATNEELETNN
                     EELQATNEELETTNEELQARTNELAEVTRNLVTERMRLLDMVELAPFYILLLYGPDLQ
                     IEAFNPRYAQLLGAREVVGLPLADVFTDPAQAELVSLVHEVYQQNTARTTPRMPIHLP
                     NEQGRLVARYFVYTIVPTHGDAGKVNGIVIYADDVSAQVADEAAERRENLKLMIEHAD
                     QVALALFDAQTRRLLQASPRYLDIAERATGYARDTILGRPWPEMAFFSSSPAEAEELF
                     QAVLAGGESRRLPGLRVRLSDEAQEAVWDCSLTPIPAGAPSPVPQVQFVVVSAVEVTQ
                     PTQALQQLTEVDRLKDEFMSLASHELRTPLVPLRGYADLLKRLIRKQETEPGWDPRIG
                     DYVGKFDQQIRYLSR"
BASE COUNT          523 a          882 c          880 g          501 t
ORIGIN      
        1 gtcatccagg acccgcaaac cgcgctctat ccgtcgatgc ccctggcgct gccgccgacg
       61 gtggtcgatt acaccgtcga tgtcgaggag atgggaccct tgctctatga gctgacgcaa
      121 gggctggcgc aaccggcgcc accgccggag ttggccgatc cgttacacga catcatggac
      181 cgggtgagcc agcagtcgag catcgacttc cggcagtata aaccggcgac catcgtgcgc
      241 cgcatcttcc gccgcatgac cgtgacgcat accgcgacta tgcgcgacta tgtgcgctac
      301 ctggatcagc acccggagga agtgggcgaa ctggtgcgat ccttcctgat caaagtgacc
      361 gagttttttc gcgaccgcga tgcctttgtc tacctgaaga atacgatcct gcccgacctg
      421 attacgcgcg cgcgggctaa ggagcgcgtg ctacgcttct ggtcggcggg ctgcgccacg
      481 ggcgaagaac cctattcgct ggccctgttg ctggccgatc tgctggggcc cgaactgggg
      541 gagtggagcg tgcgcatttt cgccaccgat ctcgacacgg atgccgtcgc ctttgcccgc
      601 cgcggggtgt acccggagaa cctgctgcgc aacctgcccg acgattaccg ccagcgcttt
      661 ttcgagccga cggatcgcgg ctttcgggtg gcgaagttct tgcgccagat ggtcattttc
      721 ggcgagcagg acctgagccg cggcgtgccc ttcccgcgca tcgatctggt tatctgccgc
      781 aacctgctga tctacttcaa gccggagttg caacagacgg tgctggacat cttcgcctac
      841 tcgctgcaac aggccaacgg ctatctcttc ctgggccagg ccgaaacggt gcggccctcc
      901 aaggcgatct atgagctggt caacaagcgc tggaagatct atcgctgttt gagcggcccc
      961 ttaccgctgt tgaatcgccc ggtggcccgg ctgccggcga ccctgccgcc cgatcgtcca
     1021 tccggcccgc gccgcccgcc ggtcgaattg acggagcttc gttcgccccc gccgcccgtt
     1081 tcggaagttg aactcggcca actgcgccgc ctgaacgagt gggtgctgcg ctttttgccc
     1141 cacggggtgg tggtgattga tcgcacgtat cgcatcctga cgatcaacgg cacggcccga
     1201 cggttgctgg gcatccggga ggcgggcgtc gaccaagatt ttttgcattc ggtgcgtggg
     1261 ctgccctatg aggaggtgcg cggcgccatt gacacggtgt ttcgtgagcg gggcggggtg
     1321 acgttgccgg aactggaggt ggaggcgacg gctagcggcg aactgcgcta cctgacactc
     1381 agcatagtcc ttatgcatct tgacaacgac ggtccggatc tggccctgat cagcgtgacc
     1441 gatctgactg aacaggtgca aacccggcgc catctggagt ctgttcagcg cgaccagaaa
     1501 caactgttgg aggaactaca ggttaccaac cggcggttcg gcgagatgaa taaagagctc
     1561 caagacgcca acgaagaact ccaggcggcc aacgaggaaa tgatgctcac gcaggaagag
     1621 ctacaagcca ccaacgagga gtttgaggcc accaacgagg agttgcaggc caccaacgag
     1681 gagttggaaa ccaataatga ggagttgcag gccaccaacg aggaactgga gaccaccaac
     1741 gaggaattgc aggcacgcac caacgaactg gcggaagtga cccgcaacct ggtgactgag
     1801 cggatgcggc tgctcgatat ggttgaactg gcacccttct acatcctgct gctgtacggc
     1861 cccgacttac agattgaagc gttcaatccg cggtatgcgc agttgctggg tgcccgcgag
     1921 gtggtgggcc tcccgctagc cgacgttttc accgatccgg cgcaggccga actggtgtcc
     1981 ctggtgcacg aggtgtacca gcagaatacc gcgcgcacca cgccgcgcat gccgatccac
     2041 ctgcccaatg agcaggggcg gctggtggcc cgctacttcg tctacactat tgtgcccaca
     2101 catggggacg ccggcaaagt caacggcatc gtgatttacg ccgacgatgt ctccgcgcag
     2161 gtggcggacg aagccgctga gcgccgcgag aatctgaagc tgatgattga acatgccgat
     2221 caggtcgccc tggccctctt cgacgcccaa acgaggcgcc tgttgcaggc cagcccgcgc
     2281 tacctggaca tcgccgaacg cgcgacgggc tatgcccgcg acacgatcct cgggcgcccc
     2341 tggccggaaa tggccttctt cagcagcagc ccggccgagg ccgaggagtt gttccaggcc
     2401 gtactggccg gcggcgagtc gcgccggttg ccgggtctgc gcgtgcgcct gagcgatgag
     2461 gcccaggagg ccgtctggga ttgcagcctg accccgatcc cggccggcgc cccttcgccc
     2521 gtgccgcagg tgcaatttgt ggtcgtctcc gccgtcgagg tgacgcaacc gacccaggcg
     2581 ctgcagcaac tgacagaagt ggatcggctc aaagacgagt ttatgtcgct ggcgagccat
     2641 gagttgcgca cgccgctcgt gccgttgcgc ggctacgccg atctgctcaa gcggctgatc
     2701 cgcaagcagg agaccgaacc gggatgggac ccccgcatcg gcgactatgt cggcaaattc
     2761 gatcaacaga tccgctacct cagccg
//
LOCUS       DASUYC010000165         2960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_59795,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000165 DASUYC010000000
VERSION     DASUYC010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59795"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..1746)
                     /locus_tag="VF276_02620"
     CDS             complement(205..1746)
                     /locus_tag="VF276_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605787.1"
                     /translation="MYLVWYDDSPKKSVQEKIDEAIDRFQDRFGFAPDQCLVSTGLDI
                     QHPHLTVRPVHYVRPHYFQVGCEDDVMAALAAHKDENVAPLVPVSVGEEPRGGRRRQA
                     APATPVAPPKPVAPVAAVAAPDAAAEPVVAPPPAPVVPVADEPAAPPIHKPVAPVAEV
                     APPAVAASKPARTHKAAPGAATPAVEAPKPKQKATSVAETPVVEPPKPARKAAAPVAE
                     APKPVRKAAAPVAEAPAVEAPKPARKAAPPVAEAPVIEAPKPARSRKAAVPPVEPVVE
                     APKPARKGATPVAGEAPVVAAPKATPKAAASAEPPVAEAPKRARKSSAGAEVPDAAAA
                     VVAVPPAPAADAPKATRTRKAATPVAEAPAPLPVPAPVAASAAEAPKPARTRKAAPVA
                     ESVPAPVAEAAPATPVADKSRLTPARPRKAAPAPEPAPVVVPPAAARKKPAAAAPVPA
                     PAPAAPRKIAAAARPEQLRQTSMFGEDLAAPLAVDRDVKAAVDAAAPRRAARKAPQPP
                     LRQAS"
     gene            complement(1840..2088)
                     /locus_tag="VF276_02625"
     CDS             complement(1840..2088)
                     /locus_tag="VF276_02625"
                     /inference="COORDINATES: protein motif:HMM:NF039800.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HEX6605788.1"
                     /translation="MYRIDEPIALLELRYNYQPRAFRWRGMIHAIYQVERRWAAVALP
                     WRPAHRQYWRVRCQDGRRYDIYQDLRANTWHLAAIRAA"
     gene            complement(2119..2673)
                     /locus_tag="VF276_02630"
     CDS             complement(2119..2673)
                     /locus_tag="VF276_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605789.1"
                     /translation="MKKFLALMAMLFVSVLAIWVAYRLAPETWAMIAGIVFGVVASLP
                     MCGIVVLLLLRGNRREPERPVETTYYQAGPPRVILNAEPAPYASFAPYGPERGPVRRL
                     PAGPAYQPAPARAAAPPRTFVDHAQPSYDYAPAREWAPEPEAWMGEDPYEMDDAGWDA
                     PAEPYAAWEDPAPHGSGRPRILGH"
     gene            complement(2686..>2960)
                     /locus_tag="VF276_02635"
     CDS             complement(2686..>2960)
                     /locus_tag="VF276_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605790.1"
                     /translation="RGTGAEGLLGKGDFLAVLGGEVIRLQAAFATGAEVGRTVALLHE
                     CAQERAGSGQPRAARRNGPMTDDEAAERRGLWKAQRGPIRLLKSGS"
BASE COUNT          381 a         1036 c         1081 g          462 t
ORIGIN      
        1 tcaaccccga aaattagcac gccgccgcta cccacccccc ttgtcactct gagcgcagcg
       61 aagagtctcc tcgcgtcgga gtaggagacg agatgcttcg ctgcgctcag cctgacaccg
      121 gggaacagca gccgggcatg acgaaggggc gggaccccag ccgcttggct ggagataccc
      181 gccccttttc gatggcgagg gcgcttacga cgcctggcgc aggggcggtt ggggggcctt
      241 gcgggccgcc cggcgtgggg ccgccgcgtc caccgccgcc ttcacatcgc ggtccacggc
      301 cagcggggcc gccaggtctt cgccgaacat cgaggtctgg cggagttgct ccgggcgcgc
      361 cgctgccgcg atcttgcgcg gtgcggcggg tgccggagcg ggaacgggcg ccgccgctgc
      421 tggcttctta cgcgcggcgg cgggcggcac gactacgggc gccggctcgg gcgcgggggc
      481 cgctttgcgc ggccgggccg gggtcaggcg cgacttgtct gccaccgggg tggcgggtgc
      541 ggcttcggcc accggcgcag gcacgctttc cgccaccggc gctgccttgc gcgtccgggc
      601 cggtttgggc gcttccgcgg cggaggccgc gaccggcgcg ggcacgggca gtggagccgg
      661 agcttcagca acgggggtcg cggccttgcg cgtccgggtt gctttcggcg catccgccgc
      721 gggggcgggc ggcacggcaa ccactgctgc ggcggcatcc ggaacctcgg ccccggcaga
      781 agacttgcgc gcccgcttcg gcgcctcggc aaccggcggc tcagccgagg cagcggcctt
      841 gggagtggct ttcggtgccg caacgaccgg cgcttctcca gcaaccgggg tggcgccctt
      901 ccgcgcgggc ttcggcgctt cgaccaccgg ctcaacgggt ggtacggcag ccttgcggga
      961 ccgcgccggc ttgggtgctt cgatcacggg ggcttcggct actgggggcg ctgctttccg
     1021 cgcaggcttc ggcgcttcta ccgcgggggc ttcggctacc ggggcagccg ctttgcggac
     1081 gggtttgggc gcttcggcca ccggcgcagc cgctttccgc gcaggcttcg ggggttccac
     1141 caccggcgtt tcggccaccg aggtggcttt ctgcttgggc ttaggcgctt caacagcagg
     1201 cgtcgcggct ccgggcgctg ctttgtgggt ccgggctggc ttggaggctg ccaccgcagg
     1261 cggcgccact tccgctaccg gtgccacggg tttgtggatg ggcggggccg ccggttcgtc
     1321 tgcgaccgga acgaccgggg caggcggcgg ggccaccacc ggttcggcgg cggcgtcagg
     1381 cgcggccacg gctgccaccg gcgctaccgg ctttggcggg gccaccggag ttgccggtgc
     1441 cgcctggcgg cgccgaccac cgcggggctc ttccccgacg gatacgggca ccagcggcgc
     1501 cacgttctcg tctttgtgcg ccgccagcgc ggccatcacg tcgtcctcgc agcccacctg
     1561 gaaataatgg ggccggacat agtgtacggg gcgcaccgtc aggtgcgggt gctggatatc
     1621 gaggccggtg ctgaccaggc attgatccgg cgcgaagcca aagcggtcct ggaaccggtc
     1681 gatcgcttcg tcgatcttct cctggacgct cttctttggc gagtcatcat accaaaccag
     1741 gtacatctct cattctccct gattactcac taaacgcagt tctgacctgg gatcgtcact
     1801 actggatcat gttacaggca cgccggtgta tacgcacact tacgccgcgc ggattgccgc
     1861 caggtgccag gtattggccc gcagatcctg gtagatgtcg taccggcgcc cgtcctggca
     1921 gcgcactcgc cagtattgcc ggtgggcggg ccgccagggc agcgcgaccg cggcccagcg
     1981 gcgctcgacc tgatagatcg cgtggatcat gccgcgccac cggaaggcgc ggggctgata
     2041 gttatagcgc agttcgagca aggcgatcgg ttcgtcaatc cgatacatgg cgtcctccct
     2101 ggcgcgcgac cggcgggatt aatgacccag aatgcgcggg cggcctgagc cgtgcggagc
     2161 cgggtcttcc caggcggcat acggctcggc aggcgcatcc cacccggcgt cgtccatctc
     2221 gtagggatcc tcgcccatcc acgcctcggg ctcgggcgcc cactcgcgcg cgggcgcata
     2281 gtcgtaggac ggctgggcgt ggtccacgaa ggtgcgcggc ggggcggcgg cgcgggcggg
     2341 cgcgggctga taggccgggc cggcgggcag gcggcgcacc gggccccgct ccggcccgta
     2401 gggcgcgaag ctggcgtacg gcgcgggttc ggcgttcagg atcacgcgcg gcgggccggc
     2461 ctggtaatag gtcgtttcca ccgggcgctc gggttcgcgc cggttgccgc gcagcagcaa
     2521 caacaccacg atgccgcaca tcggcagcga ggccacgacg ccgaacacga tgcccgcgat
     2581 catggcccag gtttcgggcg ccaaccggta cgcgacccag atcgccagca ccgacacaaa
     2641 cagcatcgcc atcaaggcga ggaatttctt catcgtgttt tctccctacg acccggactt
     2701 gagcagccgg atcgggccgc gctgggcctt ccagaggccc cgccgctcgg cagcctcgtc
     2761 atcggtcatc gggccgttgc ggcgggcggc acgcggctgg ccgctcccgg cgcgttcctg
     2821 cgcgcactca tgcagcaggg ccacggtgcg gcccacctcg gcgccggtgg cgaacgccgc
     2881 ttgcagccgg atcacctcgc cgccgagcac ggccaggaag tcgcccttgc ccagcagccc
     2941 ttcggctccc gtgccccgca
//
LOCUS       DASUYC010000166         2525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1907345, whole genome shotgun sequence.
ACCESSION   DASUYC010000166 DASUYC010000000
VERSION     DASUYC010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1907345"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VF276_02640"
     CDS             complement(<1..219)
                     /locus_tag="VF276_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6605791.1"
                     /translation="MRPINEQVVVITGASSGIGRETALQFGQRGAPVVLAARNMVALD
                     EVARQVREGGGQAQVVVADVGEWEQVRRV"
     gene            492..1370
                     /locus_tag="VF276_02645"
     CDS             492..1370
                     /locus_tag="VF276_02645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HEX6605792.1"
                     /translation="METGYIYSNGVRLYYEMIGQGDPLLLLNGGPGFPHDYLQELRAL
                     APYATLVFFDQRGTGKSDRVDPAGYTIDANVEDVENLRDALQLGPCGVLGHSWGGMLA
                     QAYVLTYPQHVTRLILADTFSSIADCNAALARMRAAMPPETQAIYDRYEREGLYAAGD
                     TYPAEYQAALDPAYEPVFISVPPPDYLQDTFAKIAYDVYRTMWGEESEFRVTGTLAAF
                     DVADRLGEIRVPTLVIAGASDMPTVAMAAATARAIPDARLEMFAHSRHFPFIEEPAKF
                     LQVVRQFLEETSPAQV"
     gene            1754..>2525
                     /locus_tag="VF276_02650"
     CDS             1754..>2525
                     /locus_tag="VF276_02650"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX6605793.1"
                     /translation="MAHYDPPVPRAATESPYLRALAALAARGVAAPQETIAAILRTAA
                     DELGMACSLLTRRLPEQNQAEVVAAYHATPGGGPTPGAILSLEAGAAPHAPFAVADTD
                     AHPARDGGPPAPIAGVRSYIGVPVRLAGGSVYGTLWTLDPQPRPFAPADTQLLEVLAH
                     LLGAAVERDRDQAARARLEQELQQQREFALQVMNTMGQGLYTADPAGRFEYVNRAFAR
                     MLGTAEPALIGRSARDVTFAPDDLLHDPQTGRPPEGLTI"
BASE COUNT          400 a          927 c          821 g          377 t
ORIGIN      
        1 gacccggcgc acctgctccc actcgcccac atcggcgacc accacctggg cctggccgcc
       61 gccctcccgc acctgccgcg cgacctcgtc cagcgcgacc atattgcgcg cagccaggac
      121 gaccggcgca ccgcgctgcc cgaactgcaa ggccgtctcc cggccaatcc ccgaagacgc
      181 gcccgtgatg acgaccacct gctcgttgat cggtcgcatc gtgttcccct cctgcttctt
      241 tgccgggcgg ttgcccttgc ccaggcccgg cgcccggcgg cctcgcggca gaccctcacg
      301 acacggtccg tactagcaag tctgtgacca cggcccaccc cgctgcgtac ggtggcgcga
      361 caaatgtggg atgatcccga cagacggacg caactctctg gcccgatcgt ctatagtaca
      421 gagcatcccg gccgccacag ccgggacagt gggtgccggg ccggccatta gcgaggaggg
      481 cgaagcgatc catggagaca gggtatatct actcgaatgg ggtgcggctc tactacgaga
      541 tgatcgggca gggcgacccg ctgctgctgc tgaacggcgg ccccggcttc ccgcacgatt
      601 atctccaaga actgcgcgcc ctggcgcctt acgccacgct ggtcttcttc gatcagcggg
      661 gcaccggcaa gtcggaccgg gtggatcccg cgggctacac catcgacgcg aacgtggagg
      721 atgtcgagaa cctgcgcgat gccctgcaac tgggaccgtg cggggtgctg gggcactcct
      781 ggggcggcat gctggcgcaa gcctatgtgc tgacgtatcc gcagcacgtc acccgcctga
      841 tcctggccga tactttctcc tccatcgccg actgcaacgc cgcgctcgcg cgcatgcgcg
      901 cggccatgcc gcccgaaacg caggcgattt atgatcgcta cgagcgcgag ggcctctacg
      961 cagccggcga cacctacccc gccgaatacc aggcggccct ggaccctgcc tacgagccgg
     1021 tcttcatcag cgtgccgccg cccgactatc tgcaagacac gttcgccaag atcgcctacg
     1081 atgtctaccg caccatgtgg ggcgaggagt ccgagttccg cgtgacgggc acgctggccg
     1141 cgttcgacgt ggcggatcgg ctgggtgaga tccgggtgcc cacgctggtc atcgccgggg
     1201 cgagcgatat gcccacggtc gctatggccg ccgccaccgc gcgggccatt cccgacgccc
     1261 ggctggagat gttcgcgcac tcgcgccatt tccccttcat cgaggaaccg gccaagtttc
     1321 tccaggtggt ccgccagttc ctggaggaga ccagcccggc gcaggtatag caaccgcgca
     1381 gcggcagccg ccgcccgacg ataactcatc gtctctcccc ttcccgcggc gggaaggggg
     1441 gccggggggt taggttggtt gggcgcccgg ctagacagcg accccatgaa ttaggtatta
     1501 tcccagtagc agcatagacc gggtcaccgc ctgccggaag cggtagccac cacagcataa
     1561 tggtgcctgt ggcacacggt cggtcgggac acaggcgatt cccggcggca gcatgttgcc
     1621 gggcacggca catcgctggg cgcaacattg atcgcacaaa cctcggagca gatgggcagg
     1681 gcgccggttt ccgcgccacc agccacccgt gcgcagccgc gccggcctgc cccggccagg
     1741 agagaaactg tcgatggcgc attacgatcc ccccgttccc cgagccgcga ccgagtcacc
     1801 ctacctgcgc gcgctggccg cgctggccgc tcgtggtgtt gccgcccccc aggagactat
     1861 cgccgccatt ctgcgcacgg cggcggacga actggggatg gcatgcagtc tcctcacgcg
     1921 ccggctgccg gagcagaacc aggcggaggt ggtggccgcg tatcacgcca cgcccggcgg
     1981 cggccccacg cccggcgcca tcctgtccct ggaggcaggc gccgcgccgc acgccccgtt
     2041 cgccgtggcc gataccgatg ctcacccggc ccgggacggc ggcccgcccg cgcccattgc
     2101 cggggtgcgc agctacatcg gcgtgccggt gcgcctcgcc ggcggcagtg tctacggcac
     2161 cctctggacg ctggatccgc agccgcgccc gttcgccccg gcggacaccc agttgctgga
     2221 agtgctggcc catctgctag gcgcagctgt cgaacgggac cgggaccagg ccgcccgcgc
     2281 ccggctggag caagaactgc aacagcagcg cgaattcgcc ctgcaagtga tgaacacgat
     2341 gggccagggg ctgtacacgg cggaccccgc cgggcgcttt gaatacgtga accgggcctt
     2401 cgcgcggatg ttgggcactg cggagcccgc cttgatcggt cggtcggcgc gcgacgtgac
     2461 cttcgccccc gacgatttgc tgcacgaccc ccagacgggc cgaccgccgg agggcctcac
     2521 catct
//
LOCUS       DASUYC010000167         2416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_292186,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000167 DASUYC010000000
VERSION     DASUYC010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292186"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2416
                     /locus_tag="VF276_02655"
     CDS             <1..>2416
                     /locus_tag="VF276_02655"
                     /inference="COORDINATES: protein motif:HMM:NF021570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HEX6605794.1"
                     /translation="LLDPARSPLNPRINGQAMHYGAMFLYIVKTVAVNASNLFNKPEW
                     LGYDGITLVGRTTSGVFDLITVFLVFLIGRRLYGRNAGLLAAVFSALAVTQIQIAHFF
                     ISEPYLVTFLTATLYFCVVLMQNRRAWAAALAGLMMGFALACKISVLPMALLIVAALA
                     LRVAYRARTRQLGPPPELDDPYGLEPATRAERTAPGWLARRRAYGLLALAALMTLIGI
                     TIGDPYGVLDSGFRVVVGNPPTVPGNTFFQLGPVQLFAEQGKYLYQLGEEAAIQRGAA
                     DVPFTRQYIGTIPVVYHFQQLVQWGMGPASGIPALFGLFAAFWFAIRRRRSAEILLLS
                     GMIPYFLTIVTLEAKWMRYMLPLVPYMCILGAALLLRGLHWSGAARDRIALRLQWGKG
                     VPLGLRLQRWAFPVLTAVAVLGSAAWATAFMNIYTHEESRVIASRWMAHNMAPGSAVG
                     HETWDDALPLNMPGDPLPPLSGVDMGLYDDKPSTEEFNYIASLLNQTDYIVLSSNRLW
                     RSIPNLPWRYPVQIRYYDLLMSGKLGFKQVLPEAYQKVYPEIFGIKINDDSADESFTV
                     YDHPQVLVFHKERSLSMDELRTLFGATLDQVSIPARHLETRTPQTYDKSLMLAEPPGQ
                     QLALGDYAWNALAQNQWVAVLLLLIVVEVFGLLAWPVTAVVCRRLPDRGYPLSKTLAL
                     VVVAWMVWMAASLRLVPFTVWSILGGVVLLGLLSAFLWRRFGSELREYMRSHRALILA
                     WEGLFLLAFGAFLLVRLLNPDLWQPWNGGEKPMEFGFLNSVLRSPWMPPGDPFYSGGY
                     INYYYYG"
BASE COUNT          345 a          831 c          798 g          442 t
ORIGIN      
        1 gttgctcgac cccgcccgca gcccgctcaa cccgcgtatc aacggccagg ccatgcacta
       61 cggggcgatg ttcctctaca tcgtcaagac cgtggccgtc aacgcgtcca acctgttcaa
      121 caagcccgaa tggctgggct acgacggcat caccctggtc gggcgaacga catccggcgt
      181 gttcgacctg atcacggtgt tcctggtgtt cctgatcggg cggcggttgt atgggcgcaa
      241 cgcggggctg ctggccgccg tgttctcggc cctggccgtc acgcagatcc agatcgccca
      301 cttcttcatc tccgagccct acctggtcac gttcctgacg gccacgctct atttctgcgt
      361 ggtgctgatg caaaatcggc gcgcctgggc cgcggcattg gccgggctga tgatgggctt
      421 cgcgctggcc tgcaagatca gcgtgctgcc gatggcgctg ctcatcgtgg ccgcgctggc
      481 gctgcgcgtg gcctatcgcg cccgcacccg ccagttgggg ccgccgcccg aattggacga
      541 cccctacggt ctggaaccgg ccacgcgcgc tgagcgcacc gcccccggct ggctggcccg
      601 gcgccgcgcc tacgggctgc tggcgctggc tgcgctgatg acgctgatcg gcatcaccat
      661 cggcgacccc tatggcgtgc tcgactccgg gttccgggtg gttgtgggca acccgccgac
      721 cgtgcccggc aacaccttct tccagttggg gcccgtgcaa ctgttcgccg agcagggcaa
      781 gtacctctac cagttgggcg aggaggcggc catccagcgc ggcgccgccg acgtgccgtt
      841 cacccggcag tatatcggca ccatcccggt ggtctaccac ttccagcagt tggtgcagtg
      901 gggcatgggc ccggcgtcgg gcatcccggc gctgttcggc ctgttcgcgg cgttctggtt
      961 tgcgatccgg cgccgccggt cggcggagat cctgctgctc agcggcatga tcccctactt
     1021 cctgaccatc gtcacgctgg aagccaagtg gatgcgctac atgctgccgc tggtgcccta
     1081 catgtgcatc ctcggcgcgg cgctgctgtt gcgcgggctg cactggagcg gggcggcgcg
     1141 cgaccggatc gccctgcgct tgcagtgggg caagggcgtg ccgctgggct tgcggctgca
     1201 gcgctgggcc ttcccggtgc tcaccgccgt ggctgtgctg ggcagcgcgg cctgggccac
     1261 cgccttcatg aacatctaca cgcacgagga atcgcgcgtg atcgcctcgc gctggatggc
     1321 ccacaacatg gcgcccggct cggcggtcgg ccacgagacc tgggacgacg ccctcccgct
     1381 gaacatgccc ggcgacccgc tgcccccgct gagcggcgtg gatatgggcc tctacgacga
     1441 caaacccagc accgaagaat tcaactacat cgccagtctg ctcaaccaga ccgattatat
     1501 tgtgctgtcg agcaaccggc tctggcgctc gatccccaac ctgccgtggc gctacccggt
     1561 gcagatccgc tactatgacc tattgatgag cggcaagctg ggcttcaagc aggtgctgcc
     1621 cgaagcctac cagaaggtct atccggagat cttcggcatc aagatcaacg acgacagcgc
     1681 cgacgagagc ttcaccgtct acgatcaccc gcaggtgctg gtgttccaca aggaacgcag
     1741 cctgagcatg gacgagctgc gcacgctgtt cggcgcgacg ctcgaccagg tgtccatccc
     1801 ggcgcgccac ctggagacgc gcaccccgca gacctacgac aagtcgctga tgctggcgga
     1861 gccgccgggg caacaactgg ccctgggcga ctacgcctgg aacgcgctgg cccagaacca
     1921 gtgggtggcg gtgctgctgc tgctgatcgt cgtggaggtg ttcgggctgc tggcctggcc
     1981 ggtgacggcg gtcgtctgcc ggcgcctgcc cgaccgcggc tacccgctga gcaagacgct
     2041 ggcgctggtc gtcgtggcct ggatggtctg gatggcggcc agcctgcggc tggtgccgtt
     2101 caccgtctgg agcattctgg gcggcgtggt gctgctgggg ctgctgtcgg cgttcctctg
     2161 gcggcgcttc gggtccgaat tacgcgagta tatgcgcagc caccgcgcgc tgatcctggc
     2221 ctgggagggg ctgttcctgc tggccttcgg cgccttcctg ctggtgcgcc tgctcaaccc
     2281 cgacctgtgg cagccctgga acggcggcga gaagccgatg gagttcggct ttctcaactc
     2341 ggtgctgcgc agcccctgga tgccgcccgg cgatcccttc tacagcggcg gctacatcaa
     2401 ttactactac tacggc
//
LOCUS       DASUYC010000168         3449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_753418,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000168 DASUYC010000000
VERSION     DASUYC010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753418"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..1616)
                     /locus_tag="VF276_02660"
     CDS             complement(84..1616)
                     /locus_tag="VF276_02660"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX6605795.1"
                     /translation="MSTTPTPSPGKVLLEMKDIGKTFPGVKALEGVSLSVREGQVHAL
                     LGENGAGKSTLIKILSGAYSKDEGQIFFDGKPVEIRQPQDAQALGIQTIYQEFNLARD
                     LTIGENIYLGHLPMRGLSVDWAQVREGSRKILDALGVALSPDAMVSTLSVAEQQLVEI
                     AKSLNRKTRILIMDEPSAVLGEKDLEKLFQVVRSLQAQGIGVIYISHRLREIFELADE
                     VTVLKDGRYVGTRQVADVTMDDLVKLMIGRDLKDVYPKREPVLGETLLEVKNLSRSKL
                     VHDISFNLRAGEIVGFAGITGSGRTEVVRAIFGADPCTAEMRVRGKPYKPSSPEGAIR
                     NGVALVTEDRKAQGLFLKQNVTVNTTISGLKLLSRFGLFLQEALELALVKRMIQNLRI
                     KTPGPNFMVINMSGGNQQKVILARCMALKPKVLIVDEPTRGIDVGAKLEIYRLIEELA
                     GAGIAILVISSELTELLGLTDRIAVMRAGEIVGELPTAEATEEAILALAMGQTATEKA
                     AA"
     gene            complement(1634..2548)
                     /locus_tag="VF276_02665"
     CDS             complement(1634..2548)
                     /locus_tag="VF276_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6605796.1"
                     /translation="MGPLAALVLAIIFFATQSPQFLRGGNFSLIIQQVVVVGTLAIGQ
                     TLVILTGGIDLSNGLIMALASVLMAGLAVNSGLDPLLAILLGFLVCIVFGLANGGLVT
                     TIGLPAFIVTLGTLNIAFALVRIYTTSTITQLPAPLLFFGTTFNLAGTDLTYGSVLMV
                     ILYVIFWYVLRATPWGRSVYAVGDNPEAARLAGINTRRVLASVYVVAAITYGIAGLLL
                     ISRTQVGDPQAGQTGNLDSITAVVLGGTSLLGGRGHIVGTLIGALIVGVFRNGLQLMG
                     VESNYQVLITGILVIVAVTVDYLSHRNR"
     gene            complement(2685..>3449)
                     /locus_tag="VF276_02670"
     CDS             complement(2685..>3449)
                     /locus_tag="VF276_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX6605797.1"
                     /translation="IAAGAKTILITPNDSKAIVPAVKKARDKGVLVIALDTATEPQTA
                     VDALFATNNFNAGLLIGQWSKAAMAGKTPVIATLDLQPGITVGAQRHNGFLQGMGLAT
                     AKPDSSDLVTAAPVVCSQDTHGDQTEGQTAMENCLSKNPNINLVYTINEPAAFGAYTA
                     IKAAGKDKDITIVSVDGGCQGVQGVVDGKIAATSQQYPLKMASLGVDAVVNFAKTGTK
                     PTGYTDTGVTLITDKAQTGVDSKDSKYGAANCWGNK"
BASE COUNT          590 a         1091 c         1128 g          640 t
ORIGIN      
        1 gagcaggctg aggttctggg tgccgacctg ggcgacgacg cgcgcccagg ggccggcgac
       61 ggggcgggcg atcggctggg cgatcatgcg gcggccttct cggttgcggt ctggcccatg
      121 gcgagggcga ggatcgcctc ctcggtcgct tcggccgtcg gcagctcgcc gacaatctca
      181 cccgcgcgca tgaccgcgat ccggtcggtg aggcccagaa gctcggtgag ctcggaggag
      241 atcaccagga tggcgatgcc ggccccggcc agctcctcga tcaggcggta gatctcgagc
      301 ttggcgccga catcgatgcc gcgggtcggc tcgtccacga tcagcacctt gggcttaagc
      361 gccatgcagc gggccaggat gaccttctgc tgattgccgc cgctcatatt gatgaccatg
      421 aagttcggac cgggcgtctt gatgcgtaga ttctggatca tccgcttaac cagggccagc
      481 tccagggcct cttgcaagaa gaggccgaag cggctcagca gctttaagcc ggagatcgtg
      541 gtgttgaccg tcacgttctg cttgagaaac agcccctgcg ccttgcgatc ctcggtcacc
      601 agggccacgc cgttgcggat ggcgccctct ggggaactcg gcttgtaggg cttgccgcgg
      661 acgcgcatct cggccgtgca ggggtcggcg ccgaagatgg cgcgcaccac ctcggtgcgc
      721 cccgacccgg taatgccggc gaacccgacg atctcccccg cgcggagatt gaaggagatg
      781 tcgtgaacga gcttgctccg cgagaggttc ttgacttcca acagggtttc gcccaagacg
      841 ggctcgcgct tggggtagac atccttcagg tcgcgcccga tcatgagctt gaccaggtcg
      901 tccatcgtca cgtcggccac ctgccgcgtg ccgacgtaac ggccgtcttt gagcacggta
      961 acctcgtcgg ccagctcaaa gatttctcgc agccggtgcg aaatgtagat aacgccgatg
     1021 ccctgggcct ggagggagcg caccacctgg aacaacttct ccagatcctt ttcgcccagc
     1081 acggcggatg gctcgtccat gatcaggatg cgtgtcttgc ggttcaacga tttggcgatc
     1141 tcgaccagtt gttgctcggc gacggagagg gttgaaacca tcgcgtccgg cgacaacgca
     1201 acaccgaggg cgtccaggat cttccgcgat ccttcccgga cttgcgccca gtctaccgag
     1261 aggccgcgca tcggcagatg ccctaggtag atgttctccc cgatcgtcag gtcgcgggcc
     1321 aggttgaatt cctggtagat ggtctggatg cccaacgcct gcgcgtcctg cggctggcgg
     1381 atttcgaccg gcttgccgtc gaagaaaatc tggccttcgt ccttgctata ggcgcccgac
     1441 aagatcttga tcagcgtcga cttcccggcc ccgttctcgc ccagcaaagc atggacttgg
     1501 ccttcacgca cgctaaggct gacgccctcc agggccttga cgccggggaa ggtcttgccg
     1561 atgtccttca tttcgagcag caccttgccc ggcgatggcg tgggagttgt gctcatggcg
     1621 gcctcctttc cggctacctg ttgcggtgcg agagatagtc aacggtcacg gccacgatca
     1681 ccaggatccc ggtaatcagc acctgatagt tcgactcgac gcccatcagt tgcaggccgt
     1741 tccggaacac gcccacgatc agcgcgccga tcaacgtccc gacgatatgg ccgcgcccgc
     1801 ccaacaggct ggtgccgccc agcaccaccg ccgtgatgct gtccaggttg ccggtctgcc
     1861 cggcctgggg atcgcccacc tgggtgcgcg agatgagcag caggccggcg atgccgtagg
     1921 tgatcgccgc cacaacgtag accgacgcca gcacgcgccg ggtattgatg ccggcgaggc
     1981 gggccgcttc gggattatcg cccaccgcgt agaccgagcg cccccacgga gtcgcgcgca
     2041 agacgtacca gaagatcaca taaaggatga ccatgagtac ggagccatac gtgaggtccg
     2101 tcccggccag gttgaaggtg gtcccaaaga acaggagcgg cgccggcagt tgcgtgatcg
     2161 tcgaggtggt atagatgcgc accagggcaa aggcaatgtt taacgtgccc agcgtgacga
     2221 tgaaggcggg cagcccgatc gtggtcacca gccccccgtt cgccaggccg aagacaatgc
     2281 acaccagaaa cccgagcagg atcgccagaa ggggatccag accggagttc accgctaggc
     2341 cggccatgag caccgacgcc agcgccatga tcaagccgtt cgagaggtcg atcccgccgg
     2401 ttaagatcac tagcgtttgg ccgatagcca gggtgcccac caccactacc tgctggatga
     2461 tcagggagaa gttgccgccc cgcaggaact ggggggattg cgtggcaaag aagatgatcg
     2521 ccaggacgag cgccgccagt ggccccatga tgggcgaccc ctggaacacc cgcagaatgt
     2581 tcgagcgacg cttggtgact tcgctcacga ctgcgctcat gggtaatcct cctgtatgag
     2641 cgagcggcgg cggacctctt ccaccgccgc tctgtcatcc cgtgttactt gttgccccag
     2701 cagttggccg cgccgtactt cgagtccttg ctgtcgacgc cggtttgcgc cttgtcggtg
     2761 atgagtgtga cgcccgtatc cgtatagccg gtgggcttcg tgccggtctt ggcaaagttg
     2821 accaccgcat cgacgcccag cgacgccatc ttcagcgggt attgctggga cgtggcggcg
     2881 atcttgccgt ccaccacgcc ctgcacgccc tggcagccgc cgtcaaccga cacgatggtg
     2941 atgtccttgt ccttgccggc cgccttgatc gcggtgtagg cgccgaaggc tgcgggttcg
     3001 ttaatggtgt acaccaggtt gatgttcggg ttcttgctca ggcagttttc catggcggtc
     3061 tggccctcgg tctggtcgcc gtgggtatcc tggctgcata ccacaggcgc agcggtcacc
     3121 aggtccgacg agtcgggctt ggcggtcgcc aggcccatgc cctggaggaa gccgttgtgg
     3181 cgctgggcgc cgacggtgat gccgggctgc aggtccagcg tggcgatgac gggagtcttg
     3241 ccggccatgg ccgccttcga ccactgcccg atcagcaggc cggcgttaaa gttgttggtg
     3301 gcgaacagcg catcaacggc agtctgcggc tcggtggctg tgtccagggc gatgacgagc
     3361 acgcccttgt cgcgcgcctt cttgacggcc ggcacgatgg ccttggagtc gttcggcgtg
     3421 atgaggatgg tcttggcgcc ggcggcgat
//
LOCUS       DASUYC010000169         2346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_792148,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000169 DASUYC010000000
VERSION     DASUYC010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792148"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VF276_02675"
     CDS             complement(<1..536)
                     /locus_tag="VF276_02675"
                     /inference="COORDINATES: protein motif:HMM:NF018154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6605798.1"
                     /translation="MSLTGDSNGQGPLVVVVAGAPGSGKTTLARYLATALTLPLFTKD
                     DIKETLFDTLGWGDRIWSRKLGTAAVALLFRCIEAELAAGRSFIVESNFHPVRANGDF
                     RALRARYQFQPCVVYCHAEEAVLLARFAARAHSADRHPGHVDQQNYDEVAAAMRGGMY
                     GPPDIGGPVIPVDTTDFAR"
     gene            complement(514..1119)
                     /locus_tag="VF276_02680"
     CDS             complement(514..1119)
                     /locus_tag="VF276_02680"
                     /inference="COORDINATES: protein motif:HMM:NF016045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HEX6605799.1"
                     /translation="MNKWFTRFAGKASELTGSSWAFLAAVAVIVIWAVTGPIFGFSDT
                     WQLVINTGTTIITFLMVFLIQNTQNRDAKAIHLKLDEIIHVIGPAHDELLDVEDRTDE
                     ELRELIRLYRRVAERKHNGGDSDEIAADARERRDENVEEGKSRIARNVDEEVLERELK
                     AAGRPADAQANGDKAPGDGSELVVSGTKEVAPARDESNRRQ"
     gene            complement(1401..>2346)
                     /locus_tag="VF276_02685"
     CDS             complement(1401..>2346)
                     /locus_tag="VF276_02685"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX6605800.1"
                     /translation="NVDYSWRMGQPYVVRSRTPLVYMVQRSRFDYYLAEQAARAGADL
                     RDGTAVTAVESDGHGATVQTAHGALHSGYVIGADGATGRVARSVGLMGDRWALPAVES
                     EVAVEPATMAYWRDKIGLDLGTVPAAYGWVFPKADHLNIGVVGAPLGEDFGSRLKAYD
                     ARHTAARVPGITRVICSRGYLLPCRRPGAPVQQGRVLLVGDAAGLVEAFSGEGIYWAI
                     RSGQLAAQAIIQEDVPGYSPALDTLLMPNLLSARHWWGLYKLCPYLCYALPRHLPVFW
                     NIVCQVVRGERHYTDIRRRLGPLGFVENWLPDPLLQLA"
BASE COUNT          368 a          812 c          769 g          397 t
ORIGIN      
        1 cgggcgaaat cggtggtatc gaccgggatg accggcccgc cgatgtcggg cgggccgtac
       61 atgccgccgc gcatagccgc ggccacctcg tcgtagttct gctgatcgac gtggcccgga
      121 tggcgatccg ccgagtgggc gcgcgccgcg aagcgcgcca gcagcacggc ctcctcggca
      181 tggcagtaga ccacgcaggg ctggaactgg tagcgcgccc gcagcgcccg gaagtcgccg
      241 ttggcgcgca ccgggtggaa gttgctctcc acgatgaacg accgccccgc ggccagttcg
      301 gcctcgatgc agcggaagag cagcgccacc gccgcggtgc ctagcttgcg cgaccagatc
      361 cggtcgcccc agcccagcgt atcgaacaac gtctccttga tatcgtcctt ggtgaacagg
      421 ggcaaggtca gcgccgtcgc caggtagcgg gccagcgtgg tcttgcccga tccgggcgcc
      481 ccggccacca cgacgacgag cggcccctgg ccgttactgt cgccggttag actcatccct
      541 ggcgggcgcg acttccttgg tgccgctgac gacgagttcc gatccgtcgc cgggggcctt
      601 gtcgccgttc gcctgcgcgt cggcgggccg gcctgccgct ttcagttcgc gctccagcac
      661 ttcttcgtcc acattccgcg cgatacggct cttgccttcc tcgacattct cgtcccgccg
      721 ttcgcgggca tcggcggcga tttcgtcgct gtcgccgccg ttgtgtttgc gctcggccac
      781 gcggcggtac aggcggatca actcgcgcag ttcctcgtcc gtgcggtcct ccacgtcgag
      841 cagttcgtcg tgcgccgggc cgatcacgtg gatgatctcg tccagcttca gatgtatggc
      901 tttcgcgtcg cggttctggg tgttctggat caggaacacc atcagaaagg tgatgatggt
      961 cgtgccggtg ttgatcacga gttgccaggt atcggagaag ccgaagatcg gcccggtcac
     1021 ggcccagatg acgataaccg ccaccgcggc cagaaacgcc cagctactgc cggtcaactc
     1081 cgatgccttg ccggcaaaac gggtaaacca tttattcatc gtggtttctc tcaagtgcta
     1141 gtgtgtcgag cgcccagcgc ccggaccggc ggtccgtcac gctccggcac atcaggtttc
     1201 cccgcacgct accgcgtctc cgcggtggat aggccgcgca tcgattggct cttgatcatg
     1261 gatcaaacga atgttgatcc atgctactaa tctaagcgta gcagtgcgcc agttgttacg
     1321 cggcaggatc agcaggcggg gtcgaccgga tctgcctctg gacagacccg gcggtagggg
     1381 ggatagcggc ggctttgggg tcaggcgagt tgcagcaagg gatcgggcag ccagttttcg
     1441 acgaacccca gcggccccag gcggcggcgg atgtcggtgt aatggcgttc gccgcgcacc
     1501 acctggcaga cgatgttcca gaacaccggc aggtgcctgg gcagggcgta gcacaggtag
     1561 gggcagagct tatacagccc ccaccagtgg cgcgcgctca gcagattggg catgagcagc
     1621 gtatcgagcg ccgggctgta gcccggcaca tcttcctgga taatggcctg cgcggcgagt
     1681 tgcccgctgc ggatcgccca gtagatgccc tcgccactga acgcctccac cagcccggcg
     1741 gcatcgccca ccagcagcac acgcccctgc tggaccggcg cgccaggccg ccggcagggc
     1801 agcagatagc cccggctgca gatcacccgc gtaatgccgg gcacgcgcgc cgcggtgtgc
     1861 cgcgcatcat acgctttaag ccggctgccg aaatcctcgc ccagcggcgc acccaccacg
     1921 ccgatgttga gatggtcggc tttggggaag acccagccgt aggcggcggg caccgtgccg
     1981 aggtcgaggc cgatcttgtc ccgccagtag gccatggtcg ccggctcgac cgcgacttcg
     2041 ctctccaccg caggcagcgc ccagcggtcg cccatgaggc ccacgctccg cgccacgcgc
     2101 ccggtggccc cgtccgcgcc gatcacgtag ccggagtgca gagcaccgtg cgccgtctgg
     2161 acggtggcgc cgtgcccatc gctttcaacc gccgtcacgg cggtgccgtc gcgcaggtcc
     2221 gcgcccgcgc gggccgcctg ctcggccagg tagtagtcga accggctacg ctgcaccata
     2281 tagaccaacg gcgtccggct gcggaccaca tacggctgtc ccatccgcca gctatagtcc
     2341 acgttg
//
LOCUS       DASUYC010000170         2194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_676198,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000170 DASUYC010000000
VERSION     DASUYC010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676198"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1305
                     /gene="glgP"
                     /locus_tag="VF276_02690"
     CDS             <1..1305
                     /gene="glgP"
                     /locus_tag="VF276_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEX6605801.1"
                     /translation="IREKVAQGIPFRDALNQVAAGGVFTTHTPVPAGNDAFSYDLMDK
                     YFNVYWPKLNISRDEFMNIARQDNSWGPTFSMTVLGLAGSRRHNGVSQLHGQVSRNMW
                     HWLWPEKKIEDVPIGAITNGVHTATWLSAELGAFYTKYLGADWYAHLDDPKTWEPLRE
                     APADELWAIHTRMKHDLVEFARTRTATRLMRMNESPGVWPILDPDALTLGFARRFATY
                     KRATMIFTDRDRLKALLNNPDRPVQIVFAGKAHPADAPGKQFIQQIYWLSKEPGFNGK
                     IIFLEEYDMNVARYLVHGSDVWLNTPRRPYEASGTSGEKAALNGLPNLSVLDGWWAEG
                     FNGKNGWAVGEGREYSNPDEQDWNDAQSLYTTLEQSVVARFYDRDATGVPQAWVQTMK
                     EAIISLAPVFSTRRMLKDYCEELYIPAIRESGAPEAALTPGR"
     gene            1442..>2194
                     /locus_tag="VF276_02695"
     CDS             1442..>2194
                     /locus_tag="VF276_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX6605802.1"
                     /translation="MALLILLVLAGWTVLQLIQPANVLTGHQGPVVGLAFSPDGRLLA
                     SAATDNSIRLWRMSDRRLDRVLTGPVTGGGAWSVTFTPDGRTVIAGYGDGQVRQWRVM
                     DGQALGALTGHTGAVTGVAVTPDGSQVVSASEDGTLHIWDTGATTLPRIITSTVGALS
                     SVAVSPDGQQIAAGGEQGQVDLWRVSDGSLVRMLSDPNAYLGHPQTRVAFAPNSEEIG
                     SAAPAYKGQVTVWRVADGILLNRINVVGDPIRA"
BASE COUNT          388 a          733 c          730 g          343 t
ORIGIN      
        1 atccgcgaaa aagtggcgca gggcatcccg ttccgcgatg ctttgaacca ggtggccgcg
       61 ggcggtgtct tcaccacgca cacgcccgtg cccgccggca acgacgcctt ctcctacgac
      121 ctgatggaca agtatttcaa cgtctactgg cctaagctga acatctcgcg cgatgagttc
      181 atgaacatcg cgcggcagga caactcctgg gggcccacgt tcagcatgac ggtgctgggg
      241 ctggccgggt cgcgccgcca caacggcgtg agccagttgc atgggcaggt gtcgcgcaac
      301 atgtggcact ggctctggcc cgagaagaag attgaggacg tgccgatcgg cgcgatcacc
      361 aacggcgtgc acaccgcgac ctggctcagc gccgaactcg gcgccttcta cacgaagtat
      421 ctcggcgccg actggtacgc ccacctggac gatcccaaga cctgggagcc attgcgcgag
      481 gcgcccgccg acgagttgtg ggccatccac acgcggatga agcacgacct ggtggagttc
      541 gcccgcacgc gcacggccac ccgcctcatg cgcatgaacg agtcgcccgg cgtctggccg
      601 atcctcgacc ccgacgcgct gacgctcggc ttcgcacgcc gcttcgccac ctacaagcgc
      661 gccacgatga tctttaccga ccgcgaccgg ctgaaggcgc tgctcaacaa cccggatcgc
      721 ccggtgcaga tcgtcttcgc gggcaaggcg cacccggccg acgcccccgg caagcagttc
      781 atccagcaga tctactggct gtccaaggag ccgggcttca acggcaagat catcttcctc
      841 gaagagtacg acatgaacgt ggcccgctac ctggtgcacg gctcggatgt ctggctgaat
      901 acgccgcgcc ggccctacga ggccagcggc accagcggcg agaaggccgc cctcaacggc
      961 ctgcccaacc tgagcgtgct cgacggctgg tgggccgagg gcttcaacgg caagaacgga
     1021 tgggccgtgg gcgagggccg cgaatacagc aaccccgacg agcaggactg gaacgacgcc
     1081 caatcgctct acaccacgct ggagcaatcc gtcgtcgcgc ggttctacga ccgcgacgcc
     1141 accggcgtgc cgcaagcctg ggtgcagacg atgaaggaag cgatcatcag cctggccccg
     1201 gtcttcagca cgcggcgcat gctcaaggac tactgcgagg agctctacat ccccgcgatc
     1261 cgcgaaagcg gcgcgccgga agctgcgctg acgcccggcc gctagccggc ccgctgggtg
     1321 ccccggtggc ggaatcgcgg ttccgccgcc ggggcgtctt catgctgtgc gaccgcacag
     1381 cccaccgtcc gcgtgaatcc cgagtaagcc aatgcgatac ctgcgatgga tcctgcccgg
     1441 catggcactg ctcatcctgc tggtcctggc cgggtggacc gtgctacaac tcatccagcc
     1501 tgctaacgtg ctgaccggcc atcaggggcc agtggtcggg ctggctttca gccccgacgg
     1561 ccggctcctg gcctcggctg ctaccgacaa tagcatccgg ctgtggcgca tgagcgatcg
     1621 ccgcctggat cgcgtgctga ccgggccggt tacgggcggt ggggcctgga gtgtcacctt
     1681 cacgcccgac ggtcgtaccg tgatcgccgg gtacggggac gggcaggtac ggcagtggcg
     1741 cgtgatggat gggcaggctc taggcgcgct taccgggcac accggtgcgg tgacgggggt
     1801 ggcggtcacg ccggatggga gccaagtcgt ctcggcttcc gaagatggca ccttgcacat
     1861 ctgggatacc ggcgcgacca cactcccacg gatcatcacc agcacggtcg gcgcactgag
     1921 cagtgtcgct gtctccccgg atgggcagca gattgcggcg ggaggcgagc aggggcaggt
     1981 agacctctgg cgcgtcagcg acggtagcct ggtgcgtatg ctgtccgatc ccaatgcgta
     2041 tctggggcac ccgcagacca gggttgcctt tgcgcccaat agcgaggaga ttggatcagc
     2101 ggcgccggct tacaaagggc aggtgacggt ctggcgggtg gcggatggaa tcttgctcaa
     2161 ccggatcaat gtcgtgggcg atccgatccg ggcc
//
LOCUS       DASUYC010000171         2180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1015012, whole genome shotgun sequence.
ACCESSION   DASUYC010000171 DASUYC010000000
VERSION     DASUYC010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015012"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..1547)
                     /locus_tag="VF276_02700"
     CDS             complement(93..1547)
                     /locus_tag="VF276_02700"
                     /inference="COORDINATES: protein motif:HMM:NF014871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX6605803.1"
                     /translation="MKQDPAEIELTLLHTNDMHGSVAGIARAARLARVIRAECAAAGR
                     PCLWWDAGDVEEWTVLESAASQGAAMWALLRAAGVDQATVGNGLLLTYGSGALAGAAA
                     AFGKPVLLAGLTELATGAPSPATAPFALHDLGGLTLGVIAITVAIREYRSLGFQVADP
                     RTVLETWLPRVRAAGADVVAVLSHCGSGRDQELAAHFGPALGWIISAHDHAVYDEPLR
                     VNGVAISETGHRGTYLGRLDIRLGRDGVRALRGTLLPLDRDEPGDPEVEAAWQRAVTA
                     GQEGLDRVVATLAAPAPLDYATESPLANLLADAIRDRTGADLAVILPGHLRHGLAAGP
                     VTVGDMLRTLGTPTHPAVVTLTGAQVRALLEWRLAPARMAAKPMPLRNKPMGQPAVSG
                     LRVTLDPDGPPGARIAALTLPDGTPLDPAALYRVGSAYYDLHFGVLDEIGPPRPTDLD
                     LAAGPLGRTLTEYLAARSPVVPPPVGRFRVAGES"
     gene            1869..>2180
                     /locus_tag="VF276_02705"
     CDS             1869..>2180
                     /locus_tag="VF276_02705"
                     /inference="COORDINATES: protein motif:HMM:NF013383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX6605804.1"
                     /translation="MTGADTGEPKITVVGTGSWGTTLAVLQARLGRQVTLLARTAEEA
                     IRLQTERQNARFLPDVPFPPGLRVEHDPAAALAGCRVLLLIAPAQTMRANVAALRPYL
                     PE"
BASE COUNT          305 a          789 c          789 g          297 t
ORIGIN      
        1 tgaattcacg ttgtaggggc cggtttgtaa ccggcctgcc gcccgaacca atcgctgccg
       61 ggactggccg gtgccccacg gttaatacag cgctatgact cgcccgccac gcggaagcgc
      121 cccacgggcg ggggcaccac cggcgagcgg gcggcaaggt actcggtcag ggtgcggccc
      181 agcggccccg cggccagatc gaggtcggtg ggccgcggcg ggccgatctc gtccagcacg
      241 ccgaaatgca ggtcgtagta ggccgacccc acacggtata gcgcggcggg gtcgagcggc
      301 gtgccgtcgg gcagggtcag ggcggcgatc cgcgcgcccg gcgggccgtc cggatcgagc
      361 gtcacgcgca gaccggagac ggcgggctgg cccatcggct tgttgcgcag cggcatcggc
      421 ttggccgcca tgcgcgccgg ggccaggcgc cattcgagca gcgcccgcac ctgcgcgccg
      481 gtcagcgtga ccaccgccgg gtgcgtcggc gtgcccaggg tgcgcagcat gtcgcccacc
      541 gtgaccggcc cggcggcaag gccgtggcgc aggtgccccg gcaggatcac cgccaggtcg
      601 gcgccggtgc ggtcacgaat ggcgtcggcc agcaggttcg ccagcgggct ttcggtcgcg
      661 tagtcgagcg gcgcgggcgc ggccagcgtc gccaccaccc ggtccaaacc ctcctggccc
      721 gcggtgacgg cgcgctgcca ggccgcctct acctccgggt cgcccggctc gtcgcggtcg
      781 agcggcagca gcgtgccgcg cagcgcccgc accccgtccc gacccagccg gatgtccagg
      841 cgccccaggt aggtgccccg gtggccggtt tcgctgatag ccacgccgtt gacccggagc
      901 ggttcgtcat agacggcatg gtcgtgcgcg ctgatgatcc agccgagcgc cgggccgaag
      961 tgggcggcca gttcctggtc gcggcccgac ccgcaatggg acagcacggc caccacgtcg
     1021 gcgcccgcgg cgcgcacccg cggcagccag gtttccagca ccgtgcgcgg gtcggctacc
     1081 tggaagccca ggctgcggta ctcacggatc gccacggtga tcgcgatgac gccaagggtg
     1141 aggccgccga gatcgtgcag cgcgaagggc gccgtggccg gggacggcgc gccggtcgcc
     1201 agttcggtca gcccggccag caacaccggc ttaccgaagg cggcggcggc cccggccagc
     1261 gccccgctgc cgtaggtcag cagcaggccg ttgcccaccg tggcctggtc caccccggcg
     1321 gcgcgcagca gcgcccacat ggccgcgccc tggctggccg cgctctccag cacggtccac
     1381 tcctcgacat cgcccgcatc ccaccagagg cagggccgcc cggcggcggc gcactcggcg
     1441 cggatcacgc gggccagccg cgccgcgcgg gcgatcccgg ccaccgagcc gtgcatgtcg
     1501 ttggtatgga ggagcgtcag ctcgatctcc gcggggtcct gcttcatctg cgcccattat
     1561 aaaaccctgc cggacgccgt gcaacgcatg gtataatagc tgtcagccgt cagccgtcag
     1621 gagacgagtg gtcagtggtc agtggtcagt ggtcagtggt cggtgatcac cgcgtcttat
     1681 cgagattaca ggtcgctatt cgttcgtcac ctgatcgcgt agcggtgcgt agcacacggc
     1741 tgttagctga cggctgatag ctgacggctg atagctatcg tccgtcacct gacggctgac
     1801 ggctgacagc tatcgtccgt cacctgacgg ctgacggctg acggctgaca gctacggagg
     1861 gcaccgccat gaccggcgcg gacaccggcg aaccgaagat caccgtggtg ggcacgggca
     1921 gttggggcac cacgctggcc gtgttgcagg cgcggctggg gcggcaggtg acactgctgg
     1981 cgcgcaccgc cgaggaggcc atccgcctgc aaaccgagcg ccagaacgcc cgcttcctgc
     2041 ccgacgtgcc cttcccgccc ggcctgcgcg tggagcacga cccggcggcg gccctcgcgg
     2101 gctgccgggt gttgctgctg atcgcgcccg cgcagaccat gcgcgccaat gtcgccgccc
     2161 tgcggcccta tctgcctgag
//
LOCUS       DASUYC010000172         3561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1863372, whole genome shotgun sequence.
ACCESSION   DASUYC010000172 DASUYC010000000
VERSION     DASUYC010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1863372"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VF276_02710"
     CDS             complement(<1..227)
                     /locus_tag="VF276_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEX6605805.1"
                     /translation="MILVVNPNPALDRVAAVRFRRGATLRPTRFSTWAGGSGVHAAHV
                     AHMLGAEVLVQGFVAGHTGALVRDHLAQDGLA"
     gene            complement(224..1192)
                     /locus_tag="VF276_02715"
     CDS             complement(224..1192)
                     /locus_tag="VF276_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007716404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX6605806.1"
                     /translation="MTGPQPPPAPDAAWVLGFDVGGTKTAAIAGSLTGQVLDRTVFAS
                     RPERGFAAMWDDMVAAGRGLIAARGQPVAVGVSIGGPLDSARGIIYSPPNLPGWDTIP
                     LKDRLVEAFGVPAYVEHDAKACALAEWLLGAARGRSQVVFLTFGTGLGAGLILDGRLY
                     RGATDHAGEVGHWRIAADGPLVYGKRGSWEGYSSGAGLAALAAYQYPDAYPPGVTAAD
                     IIAQARAGDERARELVKLSATALGQGIALLIDLLNPEVVVLGSLAVRAGDLYLPIARQ
                     VAAAEAGPEAYQACAILPAALGDQLGDVAALCAALYHGRIGPGAGP"
     gene            complement(1189..1761)
                     /locus_tag="VF276_02720"
     CDS             complement(1189..1761)
                     /locus_tag="VF276_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011138609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX6605807.1"
                     /translation="MQDETLRKQLDETIQVTQYLQTQLPKIREVATMLIEALRAGHRI
                     LAVGNGGSALDAQHLASELVGHYRTDHTPLPAFALTSDGGILTAIANDYDFESVFSRQ
                     VAALGTKGDVLVAFSTSGKSRNVLKALDKAGERGLVRLGLAGHDGGQMQGRCEYDLFI
                     PFTDTARIQEAHLMFIHLLSEYIDEAFAES"
     gene            complement(1926..2321)
                     /locus_tag="VF276_02725"
     CDS             complement(1926..2321)
                     /locus_tag="VF276_02725"
                     /inference="COORDINATES: protein motif:HMM:NF024082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HEX6605808.1"
                     /translation="MKAQLSQVRWDLVLKTSILIYILTFILGVGLSLLLPPVFNWGHI
                     DPERAVQAVVLIGPVLVIVVTGYGAWWVARKVEHAALLQGLLVGVVVALISFLLDVAF
                     SMRIDPVGLLFYALMIAAGWLGGSLGSRR"
     gene            complement(2364..3179)
                     /locus_tag="VF276_02730"
     CDS             complement(2364..3179)
                     /locus_tag="VF276_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004861860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605809.1"
                     /translation="MDARTGKRIRLGRLFNQASGRSIIIAYSHGVLLGPLPGMASLAE
                     MARVTTQLHAAEGIMIAPGLVEKLEAAFIGRDRPSLVVHLDWTNFSRSILPYEQGAQS
                     SLATIEEVAAAGADAVMTYLLLGSDDPQVEREEIARNAAVARACERWGIVHMIEPRHS
                     QERRKPETKQDLAIMRLYCRISAEIGADLVKCIWSGSVESMAAIVESCPAPVLMAGGA
                     RQERVTGAVALAEQAMAAGCRGLVFGRNIYQAPDPAGVLAALRAVVHGTGMAR"
     gene            complement(3330..>3561)
                     /locus_tag="VF276_02735"
     CDS             complement(3330..>3561)
                     /locus_tag="VF276_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605810.1"
                     /translation="LRLKETAGVPTEATIRWLGPGTIALSRTDFLETAPPQPLPGDGH
                     VFRLPLDAHALATVQVVSYQPSAVSRQVLGSG"
BASE COUNT          540 a         1268 c         1203 g          550 t
ORIGIN      
        1 gccaggccgt cctgcgccag gtggtcgcgg accagcgcgc cggtgtgccc ggcgacgaag
       61 ccctgcacca gcacctcggc gcccagcatg tgcgcgacgt gggccgcgtg gacgcccgac
      121 ccccccgccc aggtggagaa gcgcgtcggg cgcagcgtgg ccccgcgccg gaaccgcacg
      181 gcggccacgc ggtcgagcgc cgggttgggg ttgacgacca ggatcacggc cccgcgcccg
      241 gcccgatccg cccgtggtag agcgccgcgc agagcgccgc cacgtcgccc agttggtcgc
      301 cgagggcggc gggcaggatc gcgcacgcct ggtacgcctc gggccccgct tcggcggcgg
      361 ccacctgccg ggcgatgggc aggtagagat cgcccgcgcg cacggccagg ctgcccagca
      421 cgaccacctc ggggttgagc aggtcgatca gcagcgcgat gccctggcct agcgcggtgg
      481 ccgagagctt gaccagttcg cgcgcccgct cgtcgcccgc gcgggcctgg gcgatgatgt
      541 ccgccgccgt cacgccgggc gggtaggcat cggggtactg ataggcggcc agggcggcca
      601 gcccggctcc gctgctgtag ccttcccacg agccgcgctt gccgtagacc agcggcccgt
      661 cggcggcgat gcgccagtgg cccacttcgc ccgcgtggtc ggtcgcgccc cggtagagcc
      721 gcccgtcgag gatcagcccg gcgcccagcc ccgtgccgaa ggtgaggaag accacctgtg
      781 atcgcccccg cgccgcgccc aacagccact cggccagcgc gcaggctttg gcgtcgtgtt
      841 ccacataggc gggcaccccg aacgcctcga ccagccggtc cttgagcggg atggtgtccc
      901 agccgggcag gttgggcggg ctgtagatga tgccccgcgc gctgtccagc ggcccgccga
      961 tgctcacccc gacggccacc ggctgcccgc gcgccgcgat caggccgcgc ccggcggcga
     1021 ccatgtcgtc ccacatcgcc gcgaagccgc gctcgggccg cgaggcgaac acggtccggt
     1081 ccagcacctg cccggtcagg ctgcccgcga tggccgccgt tttcgtgccg cccacgtcga
     1141 agcccaggac ccaggcggcg tccggggcgg gcggcggttg cggtccggtc atgactccgc
     1201 gaatgcctcg tcgatgtatt cggacagcag gtggatgaac atcagatgcg cttcctggat
     1261 gcgcgccgtg tcggtgaagg ggatgaacag gtcatactcg cagcggccct gcatctggcc
     1321 gccgtcgtgc ccggccagcc ccaggcgcac gaggccgcgc tcccctgcct tgtctagcgc
     1381 cttcaagacg ttgcgcgact tgccgctggt gctgaaggcc accagcacgt cgcccttggt
     1441 gcccagcgcc gccacctggc gggagaacac gctctcaaag tcgtagtcgt tggcgatcgc
     1501 cgtgaggatg ccgccgtcgc tggtcagggc gaaggcgggc agcggtgtgt gatcggtgcg
     1561 gtagtggccc accagttcgg acgccaggtg ctgggcgtcc agggcgctgc cgccgttgcc
     1621 cacggccagg atgcggtgcc cggcccgcag cgcctcgatg agcatcgtcg ccacttcgcg
     1681 gatcttgggc agttgcgtct ggagatactg cgtgacctgg atcgtctcgt ccaattgctt
     1741 gcgtaacgtc tcgtcctgca tggggtctgt ccctccttcg ctacatccgg cgtgcccggc
     1801 ctccgctttg atcgcctgct taattttcac cgcggaggcg cagaggacgc agagatcgtt
     1861 aagttaagaa cggtttgcaa acgggttttg tgcgtgcacc cttgtcctgc tgacaggaaa
     1921 gctgctcacc tgcggctacc caggctgcca ccgagccacc cggcggcgat catcagggca
     1981 tagaatagca gtcccaccgg atcaattctc atgctaaagg ccacgtccaa gaggaaagag
     2041 atgagcgcga ccaccacgcc tacgagcaag ccctgtagca gcgccgcgtg ttctaccttg
     2101 cgggccaccc accatgcgcc atagcccgtc accacgatca ccagtacggg tccgatcagg
     2161 acaaccgcct gcacggcacg ctcgggatct atgtggcccc agttgaacac agggggcaag
     2221 agcaacgaca aaccaacacc gaggatgaag gtcaagatgt agatcagaat gctcgtcttc
     2281 aagacgagat cccaacgcac ctgcgagagt tgggctttca tccgcacctc tacttcgcta
     2341 cccccctggc cgggaccgca cgcctatcgg gccatgcccg tcccgtggac gacggcgcgc
     2401 agggcggcca gcacgcccgc cgggtcgggc gcctggtaga tgttgcgccc gaaaaccagc
     2461 ccgcggcagc ccgcggccat cgcctgctcg gccagggcca cggcgccggt cacgcgctcc
     2521 tggcgcgcgc ccccggccat cagcaccggc gcggggcagc tctccacgat agccgccatg
     2581 ctctcgaccg acccgctcca gatgcacttg accaggtcgg ccccgatctc ggcagagatg
     2641 cggcagtaga ggcgcatgat ggcgagatcc tgcttggtct cgggtttgcg ccgctcctgg
     2701 ctgtggcgcg gctcgatcat gtggacgatg ccccagcgct cgcaggcgcg ggccacggcg
     2761 gcgttgcggg cgatctcctc gcgctcgact tgcgggtcgt cgctgcccag cagcaggtag
     2821 gtcatgacgg cgtcggcgcc cgccgcggcc acttcctcga tggtcgccag gctgctctgc
     2881 gcgccctgct catagggcag gatgctgcgg ctgaagttgg tccagtcgag atggacgacc
     2941 aggctgggcc ggtcccgccc gatgaaggcg gcttcgagtt tctccaccag ccccggcgcg
     3001 atcatgatgc cctcggcggc gtgcagttgc gtggtcacgc gcgccatctc ggccaggctc
     3061 gccatgcccg gcagcggccc cagcaatacg ccatgcgagt aggcgatgat gatgctccgg
     3121 cccgacgcct ggttgaacaa ccggcccagc cggatgcgtt tcccggtgcg tgcgtccatg
     3181 cggcgctcct tcttcagtgg tcggtggtca gtggtcagtg gtcagccgtc agccgtcagc
     3241 tatcagccgt cagtgatata ttgggcatcg ctcgatgagt ccctaaaatc ggcaattcaa
     3301 cccaacgatg tctacaccgg ccccagcact cagccactac ctaacacctg acggctgacg
     3361 gctgacggct gatagctgac cacctgcacc gttgccaggg cgtgcgcgtc cagcggcagg
     3421 cggaagacat ggccgtcgcc cggcagcggc tgcggcggcg cggtttcgag aaagtcggta
     3481 cgggagagcg cgatggtgcc cggccctagc cagcggatcg tcgcctcggt gggcacgccc
     3541 gccgtctcct tgaggcgcag c
//
LOCUS       DASUYC010000173         5133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1676297, whole genome shotgun sequence.
ACCESSION   DASUYC010000173 DASUYC010000000
VERSION     DASUYC010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5133
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1676297"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VF276_02740"
     CDS             <1..291
                     /locus_tag="VF276_02740"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HEX6605811.1"
                     /translation="DERYHRFLITPQDMLRAEKTARAQGLSVVGFYHSHPNAPAVAST
                     YDRDHAWPWYSYIIVSVRDGQPHEMRSWTLRDDRSAFDEEDLTVAGSLAKGV"
     gene            297..1748
                     /gene="moeB"
                     /locus_tag="VF276_02745"
     CDS             297..1748
                     /gene="moeB"
                     /locus_tag="VF276_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEX6605812.1"
                     /translation="MAKILIPTALKQYAEGQEEVELDAGTVDEALADLTGRYPGLKKH
                     LYNESGALRNFVNIYLNDEDVRYLERGKTRLTADDSLSIIPAIAGGAPAQTARRPLVE
                     NGHTSLDAGEIQRYSRHLLVPEIGLDGQKKLKAASVLMIGAGGLGSPLALYLAAAGVG
                     RIGIVDFDVVDYSNLQRQIIHNTSSVGQPKLESARDRILEVNPGVQVDLYDTALSSDN
                     ALEIFAPYDVIVDGTDNFPTRYLVNDACVLLGKPNVYGSIFRFDGQASVFWAQEGPCY
                     RCLYPEPPPPGLVPSCAEGGVLGVLPGMIGIIQATETVKLLLGIGDPLVGRLLLYDAL
                     KMKFREMKLQKDPDCPICGPHPTINELIDYEAFCGIRPHTAEDDAWDITATELKRRLD
                     AGDDLVLLDVREPHEWDIAHLEGATLIPVGQVQARAGELNTADDIVVYCKGGTRSARA
                     VQELRGLGFRKLKNLKGGINAWSQDVDPSVPLY"
     gene            1968..2954
                     /locus_tag="VF276_02750"
     CDS             1968..2954
                     /locus_tag="VF276_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605813.1"
                     /translation="MTATTTPRPGRVTRIPAPVAAGARSAPRTHRWTNALGTAIVAVC
                     LLVPSILGSITDQVEATATRAYLQAPPAREPAPVPLAARDYYLVCGCVSNPIDCGKSN
                     RTAWGEYYEELSRLYAGETATAYEAPGFPQWAADHIHYVSVQTAADGPDAIAAALSAN
                     TTARSIQVIGTSAGGAALVSYLSRAMRGAFALDARLRAALIVDAPLGFAFPLRWSNLL
                     AGVEAGGMKTEVARGLGAWLRQRHIAIFAVDTPYDLIEHDPIPGVPYDPTPLYPDSDA
                     PPRSVTGDGCRGALCPLLQARDTLMARIAWHNYTGNHMPQSAQDFMAAQWAK"
     gene            2942..3652
                     /locus_tag="VF276_02755"
     CDS             2942..3652
                     /locus_tag="VF276_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605814.1"
                     /translation="MGEMIAIAEPRARQTLIHRCSRAAAAGWLLFQAGANVLLGPHIS
                     IVRTLALGSSIGVIVGLIVALLQWGALRRYVAAGWWIPATTAGCTVGGGLGAYFAYNS
                     LQEWRGFTTLLPLPQLAANLLVGAILTGGQWLVLRHWVTGTGRWVSLNLVGAGLAAPL
                     AAVAGLLTAALLNAVLGLRAWPLTLVTCYLVNGVVGSLIYTRCAALAFNAILPHPVPA
                     APAPAAWVTTWPAPDNGL"
     gene            complement(3690..4295)
                     /locus_tag="VF276_02760"
     CDS             complement(3690..4295)
                     /locus_tag="VF276_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605815.1"
                     /translation="MPRPRSRLPAIAGGLLCLLLVACTADGSDPDPRPKAINVQSGTA
                     ATPIVVYRSLAAARPAVAFALLAPTWLPAGVWPEPYVLVDAAGISPGRNGTPGVELAY
                     YVTSGNQALSIEEYSPQPPPPWADRALPGSATGLPGQEEAVSLPQGPGWLLSIALDPE
                     QPLSLTNQTIRVRFRQGAVAVILSGTRLTRDDVLHVAGSLK"
     gene            complement(4307..4771)
                     /locus_tag="VF276_02765"
     CDS             complement(4307..4771)
                     /locus_tag="VF276_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605816.1"
                     /translation="MTLAQQSQIAASLLWLALILGPPLALALGAGMVYSDGATRGLNT
                     VPVRTDNRAILVLLLITLAVWVVLLKFLYLPIVGFDAQFLSFNNLTTYDYGDIRSVQT
                     LWHLTRDWVATRPGEAMLLLSFVPLIGASVLIFARALGRAAALPGPPQRYWR"
BASE COUNT          850 a         1818 c         1695 g          770 t
ORIGIN      
        1 gacgaacgct atcatcgctt cctgatcacg ccgcaggata tgttgcgcgc cgaaaagacg
       61 gcgcgcgcgc aggggctgag cgtggtcggc ttttatcact cgcaccccaa cgccccggcg
      121 gtggcgtcga cctacgacag agatcacgcc tggccctggt actcgtatat tatagtgtcg
      181 gtccgggacg ggcagcctca cgagatgcgg tcctggaccc tgcgcgacga ccgctcggcc
      241 ttcgacgagg aggatctgac ggtcgcaggt tcgctagcaa aaggagtata gccactgtgg
      301 caaagattct gatacccacg gccctcaagc aatatgccga ggggcaagaa gaggtcgaac
      361 tggacgccgg caccgtcgat gaggcgctgg ccgatctcac cgggcgctat cccggtctca
      421 agaagcatct ctataacgaa agcggcgccc tgcgcaactt cgtcaacatc tatctgaacg
      481 acgaagacgt gcgctacctg gagcgcggca agaccaggct gaccgcggac gacagcctct
      541 cgatcatccc ggccatcgcc gggggcgcgc ccgcgcagac cgcgcgccgc ccgctggtcg
      601 agaacggcca caccagcctc gacgccggcg agatccagcg ctacagccgc cacctgctcg
      661 tgcccgaaat cggtctcgac gggcagaaga agctcaaggc ggccagcgtg ctgatgatcg
      721 gcgcgggcgg gctggggtcg ccgctggcgc tctacctggc cgcggcgggc gtgggccgca
      781 tcggcatcgt cgacttcgac gtggtcgatt acagcaacct gcaacggcag atcatccaca
      841 acaccagcag cgtgggccag cccaagctgg agtcggcccg cgaccgcatc ctggaggtca
      901 atccgggcgt gcaggtcgat ctctacgaca cggcgctgtc gtcggacaac gcgctggaga
      961 tcttcgcgcc ctacgacgtg atcgtggacg gcacggacaa cttcccgacg cgctacctgg
     1021 tgaacgacgc ctgcgtgctg ctgggcaagc ccaacgtcta cggcagcatc ttccgcttcg
     1081 acggccaggc gtcggtgttc tgggcacagg aaggcccctg ctaccgctgc ctctacccgg
     1141 aaccgccgcc ccccggcctg gtgccctcgt gcgccgaagg cggcgtgctg ggcgtgctgc
     1201 ccggcatgat cggcatcatc caggccacag agacggtcaa gctgctgctg ggcatcggcg
     1261 acccgctggt gggccgcctg ctgctctacg atgcgctcaa gatgaagttc cgcgagatga
     1321 agctgcaaaa ggaccccgac tgccccatct gcggcccgca tcccaccatc aacgagctga
     1381 tcgactacga ggcattctgc ggcatccgcc cgcacaccgc cgaggacgac gcctgggaca
     1441 tcacggcgac cgagctcaag cggcggctcg acgccgggga cgacctggtg ctgctcgacg
     1501 tgcgcgagcc gcacgagtgg gacatcgccc acctggaagg cgccacgctg atcccggtcg
     1561 gccaggtgca ggcccgcgcc ggcgaactga acaccgccga cgacatcgtg gtctactgca
     1621 agggcggcac gcgcagcgcc cgcgccgtgc aggagctccg cggcctgggc ttccgcaagc
     1681 tgaagaacct caagggcggc atcaacgcct ggtcccagga cgtggacccg agcgtcccgc
     1741 tctattaatc ccgcgccaat cccgtgcggc ccggcagctt tgccgggccg cgcggcatgt
     1801 agccgcatcc cgcgccctgc ggttgttccc cgatttgtca cctgcggttc accaccccgt
     1861 atacctggct ctgctataat gatcgcgtag cacccgcctt actcgccagc gaccctacgg
     1921 ccacgcgagc ggcgacaccc gctacagggc gcagaggcag gccggctatg accgcaacta
     1981 caactccgcg accaggacgc gtaacgcgta tccccgcgcc cgttgccgcc ggggcacgta
     2041 gcgcaccgcg cacccaccgc tggaccaacg cgctgggcac cgcgattgtc gccgtctgcc
     2101 tcctggtccc gtcgatcctt gggtccatca ccgatcaggt cgaggcgacc gccacccgcg
     2161 cctatctcca ggccccgccc gcgcgcgaac ccgcgccggt cccgctcgcc gcccgcgatt
     2221 actatctggt ctgcgggtgc gtctcgaacc cgatcgattg cggcaagtcg aaccgcaccg
     2281 cctggggcga gtattacgag gaattaagcc gcctctacgc tggcgagacg gcgaccgcct
     2341 atgaagcgcc cggtttcccg cagtgggcgg cggaccatat ccattacgtg agcgtgcaaa
     2401 ccgccgccga cggccccgat gccatcgcgg cggccctgtc cgcgaacacg acggcgcgca
     2461 gcatccaggt catcggcacc agcgcgggcg gcgcggccct ggtctcgtat ctgagccggg
     2521 ccatgcgcgg cgcgttcgcg ctcgacgcgc ggctgcgcgc ggcgttgatc gtcgacgccc
     2581 cgctcggctt cgccttcccg ctgcgctggt ccaatctgct cgccggggtg gaggccggcg
     2641 ggatgaagac cgaggtggcg cgcggcctgg gcgcgtggct gcggcagcgg catatcgcca
     2701 tcttcgccgt cgacacgccc tacgacctga tcgagcacga cccgattccc ggcgtgccct
     2761 acgaccccac cccgctgtac cctgacagcg acgccccgcc ccggtccgtg acaggggacg
     2821 gctgccgggg cgcgctctgc ccactcctac aagcgcggga caccctaatg gcgcggatcg
     2881 cctggcacaa ctacaccggg aaccatatgc cgcagtcggc tcaggacttc atggccgctc
     2941 aatgggcgaa atgatcgcca tcgccgagcc gcgcgcgcgg cagaccctga tccatcgctg
     3001 cagccgggca gcggccgcgg gctggctgct gttccaggct ggcgccaacg tgctgctggg
     3061 gccgcacatc agcatcgtgc gcacgctggc gctgggcagc agcatcggcg tcatcgtggg
     3121 gctgatcgtc gcgctgctgc agtggggcgc cctgcgccgg tacgtcgccg ccgggtggtg
     3181 gatcccggcc accacggcgg gctgcaccgt cggcggcggc ctgggcgcct acttcgccta
     3241 caactcgctg caggagtggc gcggctttac cacgctgctg cccctgcccc aactcgccgc
     3301 caatctcctg gtcggggcca tcctgaccgg cggccagtgg ctggtgctgc ggcactgggt
     3361 cacgggcacg gggcgctggg tcagcttgaa tctcgtcggg gccgggctcg cggcaccgct
     3421 cgccgccgtg gccgggctgc tcacggcggc cctgctgaac gccgtgctgg gcctgcgcgc
     3481 ctggccgctg accctggtga cttgctacct ggtcaacggc gtggtgggca gcctcatcta
     3541 cacgcgctgt gcggccctgg cgttcaatgc catcttgccc catccggtac ccgccgcgcc
     3601 cgcccctgcc gcctgggtca cgacctggcc cgctcctgac aatgggctgt aggggccggt
     3661 ttgaaaccgg cccccgcgtt aatggggtat tatttcaaac taccagccac gtgcagcacg
     3721 tcgtcgcggg tcaggcgggt gccgctgagg ataacggcga ccgcgccctg gcggaagcgc
     3781 acgcggatgg tctggttggt gaggctgagc ggctgctcgg ggtcgagggc gatggacagc
     3841 agccaaccgg gaccctgcgg caggctcacg gcttcctcct gccccggcag gccggtggcg
     3901 ctgcccggca gcgcccggtc cgcccatggc gggggcggct gcgggctgta ctcctcgatg
     3961 ctcagcgcct ggttgcccga cgtgacatag taggccagtt cgacgccggg ggtgccgttg
     4021 cggcccggcg aaatcccggc ggcatccacc agcacatagg gttcgggcca gacgcccgcg
     4081 ggcagccagg tgggcgccag cagcgcgaag gccacggcgg gccgggcggc ggccaggctg
     4141 cggtagacca cgatgggcgt ggccgccgtg cccgactgca cgttgatcgc cttggggcgc
     4201 gggtcggggt cgctgccgtc ggcggtgcag gccaccagca gcagacaaag caaaccgccc
     4261 gcgatcgccg gcaggcggga tcgcgggcgg ggcatcgcgg gcgccgttac cgccaatagc
     4321 gctgcggcgg accgggcagc gcggcggccc ggcccagcgc gcgcgcgaag atcagcaccg
     4381 aggccccgat cagcggcacg aagctcagca gcagcatggc ctcaccgggc cgcgtggcga
     4441 cccaatcgcg ggtcaggtgc cagagcgtct gcacgctgcg gatatctccg taatcgtagg
     4501 tggtgaggtt gttaaagctc aggaactggg catcgaagcc gacgatcggc agatacagga
     4561 acttgagcag caccacccag acggccagcg taatcaggag cagcaccagg atggcccggt
     4621 tgtcggttcg caccggcacg gtgttcaagc cacgggtggc gccgtcactg tagaccatgc
     4681 ccgcgcccag cgccagggcc aggggcggcc ccagaatcaa cgccagccac agcagggacg
     4741 ccgcgatctg gctctgttgt gcaagtgtca tcggttcccc cctctcggta ggtaagctgc
     4801 gatgtacgtc cgcaatacca accagggaat ctatcgcgca ctttaccacc ctggtaagtt
     4861 taataacctc tattatagca aagcaaaagc cccggcacaa gatacggctg cggaattagc
     4921 cagaaagtac cgtttgtccc tgggccatcc gaggcaaatg cccggcctgc gccgcggcac
     4981 gccgtcccaa atcgcggtat cgtcccaact tgtcattctg aacgcagcga agaatctcgt
     5041 ctcctgccca accaagacga gactcttcgc tgcgctcaga gtgacacggg gtggcgggcc
     5101 gcctgggacc gggtattaac agacgctaac acc
//
LOCUS       DASUYC010000174         3409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_521410,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000174 DASUYC010000000
VERSION     DASUYC010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521410"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VF276_02770"
     CDS             <1..240
                     /locus_tag="VF276_02770"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX6605817.1"
                     /translation="QMTLEDLVAEGDRVVDRWTMRATHTGNFAGMPATGKQFTIGGID
                     ISRLENGKIVEVWHQEDIMGMMQQLGAIPAPAAAG"
     gene            complement(313..2442)
                     /gene="mnmG"
                     /locus_tag="VF276_02775"
     CDS             complement(313..2442)
                     /gene="mnmG"
                     /locus_tag="VF276_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEX6605818.1"
                     /translation="MNEAQYDVIVVGAGHAGCEAALAAARMGRRTLLLTLNLDSVALM
                     PCNPSIGGPAKGHVVREIDALGGEMGRNTDRTFIQIRMLNTSKGPAVQALRAQCDKKL
                     YQLTMKTALETAPNLDLKQGAAAQLLLEPLAGEDAADFGGTLDPSAQAADEAIANPRR
                     RLRVTGIVTATGRTYRARSVVLTTGTFLRGRIIMGEVTASAGRAGEAPSIALAENLGE
                     LKFPLLRLKTGTPPRIDARTIDWSQVEYQFGSPTPLAFSYTPVEPVLRPPLPGYPNPA
                     PTDWRPQMPCYMVHTTAETHSVIRANLSRAPMFNGSIEGVGPRYCPSIEDKIVRFAHK
                     ESHQLFLEPEGWQTNEVYVQGANTSLPEDVQWAMLRSIPGLENAEIMRIGYAVEYDAV
                     PSTEITAALETKRVAGLFLAGQINGTSGYEEAAGQGILAGINAALSARAAEQGAPGPF
                     HPLNPRPAPLAAWPEDTLNADLTRGMAAALAEGGLLVLPRSHSYIGVMVDDLTTHVLT
                     EPYRLFTSRAEYRLLLRSDNADLRLTPIAYHLGLVDRARYEAVEAKRARIAALLARLD
                     GIRINPLAPEAAALAAQGWTLPERTVTVAEYLRRPESDWPLLPALGLTDDAEVAEQVT
                     VAAKYGPYIEKQEAEVRRLQRVEERRLPEDFDYTNVLGIRNEARQKFSQFRPATIGQA
                     ARIGGITPSDIAVLLVALERGKAPSGV"
     gene            complement(2575..3375)
                     /locus_tag="VF276_02780"
     CDS             complement(2575..3375)
                     /locus_tag="VF276_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017298172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6605819.1"
                     /translation="MSKSYLKDKVVMISGASSGIGAATARAFAAEGARLALCARRTDR
                     LDALGPELLNAGAGPVLTYPVDVRDVAQIDSFVQATMEEFGRIDILVNNAGLARRMDP
                     IASADEDAWNEMIDTNVKGLLALTRRVVPIMRDQDGGHVINLGSTAGHSVYEGGSVYC
                     ATKHAVIAITETLRLEVADRRIRVTAISPGLVETEFSVVRFQGDQGRADAVYADMTPL
                     TAEDIAECILFAANRPPHVNIDEMIIKPLDQASVNKVIRRPATEAKRD"
BASE COUNT          473 a         1152 c         1227 g          557 t
ORIGIN      
        1 cagatgacgc tggaggacct ggtcgccgag ggcgaccggg tggtggaccg ctggacgatg
       61 cgggcgacgc ataccggcaa cttcgccggc atgccggcca ccggcaagca gttcaccatc
      121 ggcgggatcg acattagccg cctggaaaac ggcaagatcg tcgaagtctg gcaccaggaa
      181 gacatcatgg ggatgatgca gcagcttggc gccatccccg cgcctgccgc agcggggtag
      241 ggcttgcccc gcggatagca cgagggcggc ccaagcgggc cgccctcgtc ttgtcttgag
      301 caggctcccg gatcagacgc cgctgggcgc cttgccgcgc tccagggcca cgagcagcac
      361 ggcgatatcc gagggcgtga tgccgccgat gcgcgccgcc tggccgatgg tggccgggcg
      421 gaactggctg aacttctggc gcgcctcgtt gcggatgccc agcacattcg tgtagtcgaa
      481 gtcttccggc aagcggcgct cttcgacgcg ctgcaagcgg cggacttcgg cctcctgctt
      541 ctcgatgtag gggccgtact tggccgccac cgttacctgc tcggccacct cggcgtcatc
      601 ggtcagcccc agggcgggca gcagcggcca gtcgctctcg gggcggcgca gatactcggc
      661 gacggtgacg gtgcgctccg gcagcgtcca gccctgggcg gccagcgcgg cggcctcggg
      721 ggccagcggg ttgatgcgga tgccgtccag ccgcgccagc agagcggcga tgcgtgcgcg
      781 cttggcctcg accgcctcat agcgggcgcg gtccaccaac cccaggtggt aggcgatggg
      841 cgtcaggcgc aggtcggcgt tgtccgagcg cagcagcagg cggtactcgg cgcgcgaggt
      901 gaacaggcgg tacggctcgg tcagcacgtg cgtagtcagg tcgtccacca tcacgccgat
      961 gtacgaatgg ctgcgcggca gcaccagcag cccgccctcg gccagggcgg cggccatgcc
     1021 gcgggtcagg tcggcgttga gtgtatcctc cggccaagcg gccaggggcg cggggcgcgg
     1081 gttcagcggg tggaacgggc cgggcgcgcc ctgctcggcg gctcgcgcgc tcagcgcggc
     1141 gttgatcccg gccaggatgc cctgcccggc ggcctcctcg tagcccgacg tgccgttgat
     1201 ctgcccggct aggaacagcc cggccacgcg cttggtctcc agcgcggcgg tgatctcggt
     1261 gctgggcacg gcatcgtact ccactgcgta gccgatgcgc atgatctcgg cgttttccaa
     1321 gccggggatc gagcgcagca tggcccactg cacgtcctcc ggcaggctgg tgttggcacc
     1381 ctggacgtag acctcgttcg tctgccagcc ctccggctcc aggaaaagct ggtggctctc
     1441 cttgtgggcg aagcgcacga tcttgtcttc gatcgacggg cagtagcgcg gccccacgcc
     1501 ctcgatgctg ccgttgaaca tgggcgcgcg gctcaggttg gcacggataa cggagtgcgt
     1561 ctcggcggtc gtgtgcacca tgtagcaggg catctgcggg cgccaatcag tcggcgccgg
     1621 gttggggtag ccgggcagcg gcgggcgcag caccggctcg accggcgtgt agctgaacgc
     1681 cagcggcgtg gggctgccga actggtactc gacctggctc cagtcgatgg tgcgggcgtc
     1741 gatgcgcggc ggggtgccgg tcttgaggcg cagcagcggg aacttgagct cacccaggtt
     1801 ctcggccagg gcgatagacg gcgcctcgcc cgcgcggccc gcactggccg tcacctcgcc
     1861 catgatgatg cgcccgcgca ggaaggtgcc cgtggtcagc acgaccgacc gcgcccggta
     1921 ggtgcgcccg gtggcggtga cgatgccggt gacgcgcagg cggcggcgcg ggttggcgat
     1981 ggcttcgtcg gcggcctggg cgctggggtc gagcgtgccg ccgaagtccg ccgcgtcctc
     2041 cccggccagc ggttccagca gcagttgggc ggcggcgccc tgcttgagat cgaggttggg
     2101 cgcggtctcc agcgcggtct tcatggtcaa ctggtagagc ttcttgtcgc actgggcgcg
     2161 cagcgcctgc acggcgggcc ccttgctcgt gttgagcatg cggatctgga tgaaagtgcg
     2221 gtcggtgttg cggcccatct cgccgcccag cgcgtcgatc tcgcgcacca cgtggccctt
     2281 ggccgggccg ccgatggacg ggttgcacgg catcagcgcc acgctgtcga ggttgagggt
     2341 cagcagcagc gtgcgccggc ccatgcgcgc ggcggccagc gcggcctcgc acccggcgtg
     2401 tcccgcgccc accacaatca catcgtactg tgcctcgttc atgctcgcca tcctacccag
     2461 cccgccgcgc gggaatgctg catactggtc acgcgcggtc caccaccgca acgtatgatt
     2521 gtaccatacc gtccgccggc ccggcaagcg cggatcgcga gcacaggaca gccatcagtc
     2581 gcgctttgct tcggtcgccg ggcggcggat gaccttgttg acgctggcct ggtccagcgg
     2641 cttgatgatc atctcgtcga tattcacgtg cgggggccgg ttggcggcga agaggatgca
     2701 ctcggcgatg tcctccgccg tcagcggcgt catatcggcg tagacggcat cggcgcgtcc
     2761 ctggtcgccc tggaagcgca ccacgctgaa ctcggtttcc accagccccg gcgagatggc
     2821 cgtgacgcgg atgcgccggt cggcgacctc cagccgcagc gtctcggtga tggcgatgac
     2881 cgcatgcttg gtggcgcaat agacgctgcc gccctcgtac accgagtgcc cggcggtcga
     2941 accgaggttg atgacgtgcc cgccgtcctg gtcgcgcatg atcggcacga cgcggcgggt
     3001 gagcgccagc aggcccttga cgttggtgtc gatcatctcg ttccaggcgt cctcgtcggc
     3061 gctggcgatg gggtccatgc ggcgggccag cccggcattg ttcaccagga tgtcgatgcg
     3121 cccgaactct tccatcgtgg cctggacgaa ggagtcgatc tgcgcgacat cccgcacatc
     3181 gaccgggtag gtaagcaccg gccccgctcc cgcgttcaat aattcgggac cgagggcgtc
     3241 gaggcggtcg gtgcgtcggg cacagagggc caggcgggcg ccctcggcgg cgaaggcccg
     3301 cgccgtggcc gcgccgatgc cgctcgacgc gccgctgatc atcaccactt tgtccttgag
     3361 atacgatttg ctcatgcttg gactcctttt ccatccgctg cccacccct
//
LOCUS       DASUYC010000175         4302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_557482,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000175 DASUYC010000000
VERSION     DASUYC010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557482"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            429..851
                     /locus_tag="VF276_02785"
     CDS             429..851
                     /locus_tag="VF276_02785"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX6605820.1"
                     /translation="MNRESAAALIRRIYEDAFNGHNPALFDELFTGDYIDYSVGYGGP
                     MDREGAKWALADYIRAFPDAHWTFEDVLVDGDRVAWRETFTGTHGQELHGIPPTGRRV
                     RAEGMSMAELRDGKVCRHWSIYDSLGIMQQIGAVPAIG"
     gene            1117..1740
                     /locus_tag="VF276_02790"
     CDS             1117..1740
                     /locus_tag="VF276_02790"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX6605821.1"
                     /translation="MAGETRRKILEATEHLAQIKGLGRVTTKEIAQQTGLSEGALYRH
                     FVHKEEVFFAILAQHLPPYLATLQTYTAGSGALSANLEGIALATMRYYRQLLPITAAL
                     FADTDLLGKYRALLHQIEGGPQTVFELVAAYIREEQQLGRITPHVPALTLAILLLGPC
                     FQYEFVRQFTGNLPFQQTDEMFVQMLVRGLAPGYFPAATPGTGPETG"
     gene            1798..2244
                     /locus_tag="VF276_02795"
     CDS             1798..2244
                     /locus_tag="VF276_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX6605822.1"
                     /translation="MAKQTQPPALLKPGNFVITTLLRAGVKIGHMSLLTVRGRKSGQP
                     RTTPVWLEERDGQRWLISPYGQVNWVRNLRAAGEATVTRGRRTERVASVELPPHEAAP
                     MLKQSLASAPSMVRAYFDVTPDSPLEDFEREVPRHPVFLLTPIREQ"
     gene            2394..3776
                     /locus_tag="VF276_02800"
     CDS             2394..3776
                     /locus_tag="VF276_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX6605823.1"
                     /translation="MEGKVAGESAPEIRAEDERREAGPPARPPDAARGRRDAAAISAA
                     QAVLQGESRRPRPARVLPFLGPAFIASIAYVDPGNFATNIQGGAKYGYLLLWVIVAAN
                     LMAMLIQALSAKLGIATGCNLAEVCRAEFAPPVVWAMWGVSEVVAMATDLAEFLGAAI
                     GLDLLFHTGLFPAALLTGVATFVILSLERRGFRPLEAVITAMIGIIAACYVVETLLDR
                     PDWGAIALHAVTPQFAGPESIVLAAGILGATVMPHAIFLHSALTQHRIVVRDDRQRRR
                     LFRFEQLDVGIAMGLAGLVNAAMLIMAASTFYSSGQTQVGSIEEAYRTLQPLLGPAAG
                     TIFAVSLLASGLSSSSVGTMAGQVIMQGYLHRKIPVWIRRLATMLPALIVIAIGVDPT
                     WALVVSQVVLSFGIPFALIPLVLFTRRPDLMGNLVNRPLTTLLATLVAAVIVSLNVYL
                     LYATFFGGGL"
     gene            3773..>4302
                     /locus_tag="VF276_02805"
     CDS             3773..>4302
                     /locus_tag="VF276_02805"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6605824.1"
                     /translation="MSRPAGFRRILVPLDGSRLAESVLPMVTLLARDLGAQIWLLHVV
                     EERPPQAVHGEPHLTTAGAAEAYLAGVAARLGPGLAVETHVHARGEPNVAASIALHVV
                     ELQADMIALCTHGPSDVRRVVAGSMGQQVGRRATTPVLLVRPQQPAPAQIRDLLVPLD
                     GTPAAACALPAASTLAR"
BASE COUNT          696 a         1521 c         1386 g          699 t
ORIGIN      
        1 cgccgggttg tccaggaaaa gctgccgcag ggcaccccgg cgatcccccc gatagccggc
       61 cagcagcacc atgtgctgct ccttgccgcg ctacccgcgc ccggccccag gaacgccggc
      121 atgggcggga ccgtggcgga ttcgccaatg gtgtagcaga gatcggacca ttacgggcgt
      181 gtgcgagtcc cgccggggct aagggcggcc agccggcagg gtaacagact cgggcttctt
      241 cacgatcctg taacgcatgg atacccgcct gttgacgttg ggcagcgaca atagaattgg
      301 gcgagtctaa ctgtcgccgg ctgcgatatt gctcccggtg gcgggctagg ggccggcagc
      361 agggcggcac ccggcgcccc gaggaatatc ctgccctgcc cgccaacgat agaaggagtc
      421 cgcggactat gaaccgagaa tcagcggcgg ccttgatccg ccggatctac gaagacgcct
      481 ttaacgggca taaccctgcg ttgtttgacg agctattcac cggcgactat atcgactatt
      541 cagtgggcta cggcggcccg atggatcggg aaggggccaa gtgggcgctg gccgactata
      601 tccgcgcctt cccagatgcc cattggacct tcgaggatgt gctcgttgac ggcgaccggg
      661 tggcctggcg ggagaccttc accggcacgc acgggcaaga gttgcacggc atcccaccca
      721 ccggccgcag agtgcgggcc gaggggatga gcatggccga actgcgcgac ggcaaggtct
      781 gccggcactg gagcatttac gacagcctcg gcatcatgca gcagatcggg gccgtccccg
      841 ctattggcta gccgccggcg cgacatcatc atgcgcgctg cgacaccctg cgcctctgtg
      901 ctgcatagac cgcaccattt ccctatccgc tgttcaccgc cagggaacga agaacgcgaa
      961 ggctatctgt ataagccttg ttcctcgtgt tcttcgcgtc tgcgcggtgc cagataagcc
     1021 cgtctgaaca gatgacagcc attgcctctt gacagccggg tctttccgag atataatgtg
     1081 agtgagtact cacacacaac gacgaaagga ccggctatgg cgggagaaac acgacggaaa
     1141 atcctggagg ccaccgagca cctggcgcag atcaaagggc tgggccgggt gacgaccaag
     1201 gagatcgcgc agcagaccgg cctgtccgaa ggcgcgctct accggcactt tgtgcataag
     1261 gaggaggtgt ttttcgccat cctggcccag cacctgcccc cctatctcgc caccctgcaa
     1321 acctacaccg ccggcagcgg cgcgctcagc gccaatctgg agggcatcgc cctggcgacc
     1381 atgcgctatt accggcagtt gctgcccatt accgcggccc tgttcgccga caccgatctg
     1441 ctgggcaagt atcgcgcgtt gctgcatcag atcgagggcg ggccgcagac cgtgtttgaa
     1501 ctggtggcag cctatatccg cgaggagcag caactcgggc gcatcacacc gcatgtgccc
     1561 gccctgaccc tggcgatcct gctgctcggc ccgtgctttc agtacgagtt tgtgcggcag
     1621 tttaccggca acctaccctt tcagcagact gatgagatgt tcgtgcaaat gctggtgcgc
     1681 ggcctggcgc cgggctattt ccccgccgct acgccgggca ccggcccgga aaccggatga
     1741 cccgcccaca ggttcaacgc gccggacagc ccggcgcgga aaggaaagca caatcccatg
     1801 gcaaagcaga cacaaccgcc ggccctgctc aaacccggca atttcgtcat cacaaccctg
     1861 ctgcgtgcgg gggtcaaaat cgggcacatg agcctgctga cggtgcgcgg gcgcaaaagc
     1921 gggcaacccc ggacgacgcc ggtctggctg gaggaacgcg acggacagcg ctggctgatc
     1981 tcgccctacg gccaggtcaa ctgggtgcgc aacctgcgcg ccgccgggga ggccaccgtc
     2041 acccgcgggc ggcgcactga gcgcgtcgcc tcggtcgaac tgccgccaca tgaggccgcg
     2101 ccgatgttga aacagagcct ggcctccgcg ccgtcgatgg tgcgggccta ctttgacgtg
     2161 acgcccgact cgccgctcga agactttgag cgcgaggtgc cccggcaccc ggtgttcctg
     2221 ctgacaccga tccgcgagca gtaaaccacc acaagaatga ctggcagggc cgatttgcta
     2281 ccggccccgc cgtcacccga ttcccggtgg aatatctcct gctgtagccc tgccagggcc
     2341 gggtagcgcc gccgtgacgc gcccatgatg ccgcacgaac ggagaggcgg gggatggaag
     2401 gcaaggtagc gggcgagagc gcgccggaga tccgcgccga ggacgagcgc cgggaggccg
     2461 ggccgcccgc gcgcccgccg gatgctgcgc ggggccgacg cgacgccgcc gccatctccg
     2521 ccgcgcaggc cgtgcttcaa ggcgagagcc gccggccccg ccccgcccgc gtgctgccct
     2581 tcctcggccc ggccttcatc gcctccatcg cctacgtcga ccccggcaac ttcgccacga
     2641 acattcaggg cggggcgaaa tacggctacc tgttgctctg ggttattgtc gcggccaacc
     2701 tcatggcgat gctcatccag gcactgtcgg cgaagctggg catcgccacc ggctgcaatc
     2761 tggccgaggt ctgccgggcc gagttcgcgc cgccggtggt ctgggccatg tggggcgtct
     2821 cggaggttgt cgccatggcg accgacctgg ccgagttcct gggcgcggcc atcgggctgg
     2881 acctgttgtt ccacaccggc ctgttccccg ccgcgctact caccggcgtc gccaccttcg
     2941 tcatcctcag tctggagcgg cgcggcttcc ggcccctgga agcggtcatc accgccatga
     3001 tcggcatcat cgccgcctgt tacgtggtcg aaaccttgct cgaccggccc gactggggcg
     3061 ccatcgcctt gcacgccgtc acgccgcagt tcgccggccc cgagagcatt gtgctcgccg
     3121 ccggcatcct gggcgccacg gtcatgcccc acgccatctt cctgcattcg gcgctgaccc
     3181 agcaccgcat cgtcgtccgc gacgaccgcc agcgccggcg cctgttccgc ttcgagcaac
     3241 tcgacgtggg catcgccatg ggcctggccg ggttggtcaa cgccgccatg ctaatcatgg
     3301 ccgcctcgac cttctacagc agcggccaga cgcaggtggg cagcatcgag gaagcctatc
     3361 gcacgctgca acccctgctc ggcccggcgg cgggaaccat tttcgccgtg tcgctgctcg
     3421 cctccggcct ctcctcgtcc agcgtcggca ccatggccgg gcaggtgatc atgcagggct
     3481 acctgcaccg caagatcccc gtctggattc gccgcctcgc caccatgctg cccgcgctga
     3541 tcgtcatcgc catcggcgtc gatcccacct gggcgctcgt agttagccag gtggtcttga
     3601 gcttcggcat ccccttcgcg ctgatcccgc tggtgctgtt cacccgccgc cccgacctga
     3661 tgggcaatct ggtcaaccgc ccgctgacga cgctgctggc cacgctggtc gccgcggtga
     3721 tcgtcagcct gaacgtctat ctgctctatg caaccttctt cggaggtggc ctatgagccg
     3781 gcccgccgga ttccggcgca tcctggtgcc gctggacggc tcgcgcctcg ccgagtcggt
     3841 gctgccgatg gtgacgctgc tggcgcgcga tctcggcgcg cagatctggc tgctgcacgt
     3901 ggtggaggaa cgcccgccgc aggccgtcca cggcgaaccg catctgacca cggcgggagc
     3961 ggcggaagcc tacctggccg gcgtggcggc gcggctggga ccgggcctgg ccgtggaaac
     4021 gcacgtccac gcccgcggcg agccgaacgt cgcggcgagc atcgccctgc atgtggtcga
     4081 actccaggcc gatatgatcg ctctgtgcac ccacggtccc agcgacgtgc gccgggtggt
     4141 ggcgggcagc atgggccagc aagtgggccg ccgcgccacc accccggtgc tgctggtgcg
     4201 cccccagcaa ccggcccccg cccagatccg cgatctgctg gtgccgctgg acggcacgcc
     4261 cgccgccgcc tgcgcgctgc ccgccgcctc cacgctggct cg
//
LOCUS       DASUYC010000176         3339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1444258, whole genome shotgun sequence.
ACCESSION   DASUYC010000176 DASUYC010000000
VERSION     DASUYC010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1444258"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..635)
                     /locus_tag="VF276_02810"
     CDS             complement(87..635)
                     /locus_tag="VF276_02810"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015236077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX6605825.1"
                     /translation="MSKHLYLVRHCQAAGQDPDSPLTPEGTAQAAALARFLGRPPVDR
                     IVSSPFLRARASIMPLARHLDLPIEIDQRLAERVLAGPDPLPDWRDHLRAGFADPDLC
                     LPGGESSRTAQARGVAALRDILDHPAQHTVIVTHGNLLALLLQHFDGAYGFEAWQRLT
                     NPDVFIADVQEDAAAVRRIWPE"
     gene            811..1683
                     /locus_tag="VF276_02815"
     CDS             811..1683
                     /locus_tag="VF276_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012412335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
                     /protein_id="HEX6605826.1"
                     /translation="MNKPMAVAENVKNEAEAKGRHAAANPWVERLARFGYAVRGILYA
                     VVGLLAVQVALGAGGATTDKHGAIATISRQPFGEVLLVLIAVGLTGYSLWGFIRAILD
                     PLGRGTDPKGIAQRIGYLISGLSYGTLVIPTVRFIMGAGGDATSNASVDMTATLLSQP
                     FGQWLAALVGVIGMAGGLGQMYQAYSTDFKKDFKSSEMSPNEMKLMTWVGRIGMAARG
                     LVFVMLGFFVLQAGLQANAQQAKGLDGALATLAAQPFGPWLLGAVALGLVAFGAYSLL
                     CARWIRVTPGDKAK"
     gene            complement(1786..2367)
                     /locus_tag="VF276_02820"
     CDS             complement(1786..2367)
                     /locus_tag="VF276_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HEX6605827.1"
                     /translation="MVNRTAQRETVIGVFNDQMTAQRAIERLKDAGFAPSDIGILMQN
                     QQGAQNLAAQTGTKAGEDAGKGAVAGGLLGALGGLLVGVGALAIPGIGPVVAAGPLAA
                     GLGAVLGSTATGAVVGAGVGAIAGALIGMGVPENEARVYEDRFRQGRILVTVKAGFNR
                     YDEAWRILRNAGAEDINFNNPQYGTQPYTNPTV"
     gene            2719..3255
                     /locus_tag="VF276_02825"
     CDS             2719..3255
                     /locus_tag="VF276_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605828.1"
                     /translation="MQGNGTITILIVGALILFALFRRAKGLLTRQKVRPNTMAVRLGI
                     FAVLGVAILLLTLNNAPILLGDLIGLALGAGLAWVGLRLTTFDRQPDGLYYTPNKYIG
                     IGVFALFAIRLIYKLVVGLSAAETPAGTTPRGAGLNALSQFSSDPLTTAVYFLLIGYY
                     AFYYTMLILRYRQTAATG"
BASE COUNT          546 a         1104 c         1121 g          568 t
ORIGIN      
        1 cgcacgagac gagactcttc gctgcgctca gagtgacatg gatacctggg aactgaaccc
       61 ctgcgagtcc atggggcaga caggggctat tccggccaga tccggcgaac ggccgccgca
      121 tcctcctgca catccgcgat aaacacatcg gggttggtga gccgctgcca ggcttcgaag
      181 ccgtaggcgc cgtcgaagtg ctgcaacagc agcgccagca ggttgccgtg ggtcacgatc
      241 acggtgtgct gcgcggggtg gtccaggatg tcccgcagag cggccacgcc acgggcctgg
      301 gccgtgcggc tcgattcgcc gccgggcagg cagaggtcgg ggtcggcgaa gccggcgcgc
      361 aggtgatccc gccaatcggg cagcgggtcg ggcccggcca gcacgcgctc ggccaggcgc
      421 tggtcgatct cgatgggcag atccaggtgg cgggccagcg gcatgatcga cgcgcgggcg
      481 cgcaagaagg ggctggacac gatgcggtcc acgggcgggc gccccaggaa ccgggcgaga
      541 gccgccgcct gcgcggtgcc ctccggggtg agcggcgagt cggggtcctg gccggcggcc
      601 tggcagtgcc gcaccaggta gaggtgcttg gacaatcctt tcctccatcc aatggcgaaa
      661 gcgtcatggc acgacttttg aaagtagagg ctgagtacgg caaatattat accgtgccca
      721 ccttgaaaac aacagccgcc ccgtcccgct ccggtgcgtg gccccatggc accgcgcccc
      781 tgtcaggccg gcgggaaagg attggatgta gtgaacaaac ccatggccgt ggccgaaaat
      841 gtgaaaaacg aggcggaagc caaaggccgg cacgccgccg ccaatccctg ggtcgagcga
      901 ttggcccgct tcggctacgc ggtgcgcggc atcctctacg ccgtggtcgg gctgctggcc
      961 gtccaggtcg ccctgggcgc gggtggcgcc acgaccgaca agcacggcgc catcgccacg
     1021 atcagccgcc agcccttcgg cgaggtgttg ctggtgctga tcgccgtcgg gttaaccggc
     1081 tactcgctgt ggggctttat ccgcgcgatc ctggaccccc tgggccgcgg caccgatccc
     1141 aagggcatcg cccagcgcat cggctacctg atcagcggac tgtcctacgg gaccctggtg
     1201 atccccaccg tgcgcttcat catgggcgcg ggcggcgacg cgacctcgaa cgccagcgtc
     1261 gatatgaccg ccaccctgct cagccagccg ttcgggcaat ggctggcggc cctggttggg
     1321 gtgatcggca tggccggcgg cctgggccag atgtaccagg cttatagcac cgacttcaag
     1381 aaagatttca agtcgagcga gatgagcccc aatgagatga aactgatgac ctgggtgggc
     1441 cggattggga tggccgcgcg gggcctggtg ttcgtcatgc tcggcttctt cgtgctgcaa
     1501 gccggcttgc aggcgaacgc ccagcaggcc aaaggcctcg acggcgccct ggcaacgctg
     1561 gccgcgcagc ccttcggtcc ctggctgctg ggcgcggtcg ccctcggcct ggtggcgttc
     1621 ggcgcctatt cgctgctctg cgcccgctgg atccgcgtga cgccgggcga caaggcgaag
     1681 taaacggggt cacgcgcggg cacaaaacga gccgggtggt ttaccacccg gctccctttc
     1741 atggcatccc gccgcccgta ggcgacggga cggcgcccgg cctgactaga cggtcgggtt
     1801 ggtgtacggc tgcgtgccgt actgcgggtt gttgaagttg atgtcctccg cgcccgcgtt
     1861 gcgcagaatg cgccaggcct cgtcgtagcg gttgaacccc gccttcaccg tgaccaggat
     1921 gcggccctgg cggaagcggt cctcatacac gcgggcctcg ttctcgggca cgcccatgcc
     1981 gatcagggcg ccggcaatcg cgcccacgcc cgcgccgacc accgcgccgg tggcggtgct
     2041 gcccagcacg gcgcccaggc ccgcggccag cggccccgcc gccaccaccg ggccgatgcc
     2101 cggaatcgcc agcgcgccga cgccaaccag caagccgccg agggcaccca gtagcccgcc
     2161 ggccacggcg cccttcccgg cgtcctcgcc ggccttggtg cccgtctgcg cggccagatt
     2221 ctgcgcgccc tgctgattct gcatcaagat gccgatgtcg ctgggcgcga acccggcatc
     2281 cttcagccgc tcaatggcgc gctgagccgt catctgatcg ttgaacacgc caatgactgt
     2341 ctcgcgttgt gctgtccgat taaccatgag ttaaacctcc ttggttccct acgatgccgc
     2401 gggccgcgta cctgaccggt gatacaggtc gcgtaacaag tgctgcccgc cggcatgcat
     2461 aagcacagcc cgtctgccaa aaacatcggg ggcaggctgt gcggggcgaa tgtccatact
     2521 taaatatgag caattactat gccacaacgt actattcttt ctagtaaatg gtggcagcag
     2581 gccgtctcgc ttgacccctg ttgcggatgg gtgtaacgtg ggtgtaatat gccccgtata
     2641 ggctatattg agatatccgc ggcctggtga tatggcgccg cttccggcgg tggcatgtac
     2701 ggaacaagga gcgtttctat gcaaggcaac ggcaccatca ccatcctgat tgtgggtgcc
     2761 ctgatcctgt ttgccctctt tcgccgggcc aaggggttgc tgacccgcca gaaggtccgc
     2821 ccgaatacca tggcggtacg gctgggcatt ttcgccgtgc tgggcgtggc gatcctgctg
     2881 ctcacgctga acaacgcccc gattctgctg ggggatctga tcgggctggc gctgggggcc
     2941 ggcctcgcct gggtgggctt gcgcctgacg acctttgacc ggcagccgga cgggctgtac
     3001 tacacgccga acaagtacat tggcatcggg gtattcgcgc tctttgcgat ccggctgatc
     3061 tacaaactgg tcgtcgggtt gagcgccgcg gagacgccgg cgggtacgac gccgcgcggg
     3121 gcgggcctca atgccctgtc gcagttcagc agcgatcctc tgaccacggc ggtctatttc
     3181 ctcctcatcg gctactacgc cttctattac acgatgctca tcctgcgcta ccggcagacg
     3241 gcggcaaccg ggtgacggcg ccgcggctcg ccgggcagcg ccgtcagcgc accaggttgg
     3301 ggtaaagccg cgcggcaagc gacacaatca gcacgttgg
//
LOCUS       DASUYC010000177         4705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_310693,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000177 DASUYC010000000
VERSION     DASUYC010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4705
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310693"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VF276_02830"
     CDS             <1..195
                     /locus_tag="VF276_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605829.1"
                     /translation="PANEPRPGTGPLTPPGARPGTGPLGEGASRRTGTTGKLNLPSRG
                     GGSSGRTGTAPLQWPKDKEE"
     gene            197..1360
                     /locus_tag="VF276_02835"
     CDS             197..1360
                     /locus_tag="VF276_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605830.1"
                     /translation="MSLYYPTRQPTIAPNIMVRKQRRLPVAGDILVRIGSRVEPDDVV
                     ARASIPGTPLTVDVAGPLGVKPKAAQRLLARPTGNTFEAGDVLASRRQSLTKRLTVKS
                     PVAGTLTGYDATTGLATVTPASTNFELQAYIDGVVTEHLPYRGVVIDTPAAVVRGIVG
                     VGGERHGVLQVAVADPAEELMPDQISARYAYAIVLGGGTTTAETLRRAVEHNVRALIV
                     GSIPEAELRAFLGYGDGLRGWTLGRTGWDFPPQAPGAPAVPPLTLIVVEGFGRVPMAA
                     KAWELLAAHDGGEVAVDGTTRLREGLIRPEVIVPLPRASGVAPYETTAPAIGVRSLVR
                     LIAPPYLGRVGRVLSVSQGKQPVPSGINTNAAEVQLPDGATIWVPLADLEVLE"
     gene            1362..2177
                     /gene="panB"
                     /locus_tag="VF276_02840"
                     /pseudo
     CDS             1362..2177
                     /gene="panB"
                     /locus_tag="VF276_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
     gene            2284..3132
                     /gene="panC"
                     /locus_tag="VF276_02845"
     CDS             2284..3132
                     /gene="panC"
                     /locus_tag="VF276_02845"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX6605831.1"
                     /translation="MQTFRTIGELRAARAAVGPGTRVGLVPTMGYLHAGHLSLVEQAR
                     NECDLVVVSIFVNPTQFGPTEDLSRYPRDEARDLQFLESAGVDWVFIPAAEEIYPPGF
                     QTYVDVREVTTVLEGAARPGHFAGVATVVAKLFNLVQPGVAYFGQKDAQQVVVIRRMV
                     RDLNFPVDLVIGMTIREPDGLALSSRNVYLDPAERQAALVLSRALRAAHDLWDAGARD
                     GERLRAAMRAVLAAEPLAQPDYVSIADPDTLAELNAIPPGQGALASLAVRIGRGTRLI
                     DNHILE"
     gene            3274..4071
                     /locus_tag="VF276_02850"
     CDS             3274..4071
                     /locus_tag="VF276_02850"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX6605832.1"
                     /translation="MLLAIDVGNTNAVIGIYEGATLRASWRAATDHERTADEWGGLIY
                     TFLSARGMALREVHACIVSSVVPPVTSALREMSAGLLGHPAMVIEPGLKTGVRIAIDN
                     PREVGADRIVNAVAAYRRYGGPCIVVDFGTATTVDAIGADGAYLGGAIAPGLTTSAEA
                     LYRLAARLYSVELVAPRSVIGKNTVNSMQAGIVIGYVALVEGLVGRMKAELQAGGAPA
                     DLPVIATGGLSGLIAHETAVITHTDPDLTLDGLRMIYEMNAHQQGAV"
     gene            4068..>4705
                     /gene="coaBC"
                     /locus_tag="VF276_02855"
     CDS             4068..>4705
                     /gene="coaBC"
                     /locus_tag="VF276_02855"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX6605833.1"
                     /translation="MSAFQDKRIVLGVCGGIAAYKVADLASKLTQAGAQVDVIMTAAA
                     QRFVTPLTFAALTHREVRTDLYESWLGEDTGHVALAHQAAALVVAPATAHTIARLALG
                     LGDDFLSAVALATTAPLLIVPAMEHNMYHHPATQGHLATLRERGALVMEPGAGRLASG
                     ATGTGRLPETPQILGALRVLLGRGGPLAGQRIVITAGGTQEPLDPVRYVGNRS"
BASE COUNT          730 a         1738 c         1581 g          656 t
ORIGIN      
        1 ccggcgaacg aaccgcgccc cggcaccggc ccgttgaccc cgcccggcgc ccgtcccggc
       61 accggcccgc tgggcgaagg cgcatcccgg cgcaccggga cgaccggcaa actgaacctg
      121 cccagccgcg gcggcggcag cagcgggcgc accggcacgg ccccgctgca atggccgaag
      181 gacaaggagg agtaaggtga gcctctacta cccgactcgc cagcccacca tcgcccccaa
      241 tatcatggtg cgcaagcagc gccggctgcc ggtggccggc gacatcctgg tgcgcatcgg
      301 atcgcgtgtc gagccggacg acgtggtggc gcgcgccagt atccccggca cgccgctcac
      361 cgttgacgtg gccgggccgc tgggcgtcaa gccgaaggcc gcgcagcgcc tgctggcccg
      421 ccccaccggc aacacgttcg aggccggcga cgtgctggcc tcgcgccggc agagcctcac
      481 caagcgcctc accgtcaaga gcccggtggc gggcacgctg accggctatg acgccacgac
      541 cggcctggcg accgtcacgc ccgccagcac caatttcgag ttgcaggcgt acatcgatgg
      601 cgtggtcacg gaacacctgc cctatcgcgg ggtggtgatc gacacgcccg ccgccgtggt
      661 gcgcggcatc gtcggcgtgg gcggcgagcg ccacggcgtg ctccaggtcg ccgttgccga
      721 tcccgccgaa gagttgatgc ctgaccagat cagcgcgcgc tatgcctacg ccatcgtgct
      781 gggcggcggg accacgaccg ccgagacgct gcgccgggcc gtggaacaca acgtgcgcgc
      841 cctgatcgtg ggcagcatcc cggaggccga actgcgcgcc ttcctgggct acggcgacgg
      901 cctgcgcggc tggacgttgg gccgcaccgg ctgggatttc ccgccgcagg cgcccggcgc
      961 gcctgccgtg ccgccgctca ccctgatcgt cgtcgagggc tttggccgcg tgcccatggc
     1021 cgccaaagcc tgggaactgc tggccgcgca cgacggcggc gaggtggccg tggacggcac
     1081 cacccgcctg cgcgagggcc tcatccggcc cgaggtcatc gtgcccctgc cgcgcgccag
     1141 cggcgtcgcg ccctatgaga ccaccgcacc cgccatcggc gtccgctcgc tggtgcgctt
     1201 gatcgcgccg ccctatctag gccgggtcgg gcgggtgctg agcgtctcgc agggcaagca
     1261 acccgtgccc tcgggcatca acaccaacgc cgccgaggtg caattgccgg acggggccac
     1321 catctgggtg cccctggccg acctggaagt attggagtaa catgcgcgtc tcgattaccc
     1381 agttacagca aatgaaaaag cgcgggcagc gcatcgtcat ggtcacggtc tatgactaca
     1441 gcaccgcgcg cctcaccgcc gatgccggca tccccgtggt gctcgtcggc gattcgctgg
     1501 gcatggtcat gctcggctac gactcgacca tcccggtgac gatggacgtg atggtccacc
     1561 acaccgccgc cgtggtgcgc ggcaacacca aatcgctagt catcggcgat atgcccttcc
     1621 tcagctatcg cggcagttcg gacgacgcct tgcgcaacgc cgggcgcttc ctgcaagagg
     1681 ccggggcggg cgcggtcaag gtcgagggcg cagacaccgt gccgacgatc cggcgcatgg
     1741 tggccgccgg catcccggtc atgggccacc tgggcctgac gccgcaatcg gtcaaccagt
     1801 tcggcggctg gaaggcgcag ggcaagaccc tcgccgccgc cgcgcgcatg ctcgacgatg
     1861 cgctggcgct gcaagacgcg ggcatcttcg ccctcgtgct cgaaaccgtg cccgcgaccc
     1921 tggcccgcat catcagcgaa cgcctcgcca tcccgaccat cggcatcggc gccgggccgc
     1981 attgcgacgg gcaggtccag gtgctgcacg acctgctcgg ctggtatccc gacttcatgc
     2041 ccaagcacgc gcggccctat gcccagatcg gcgacgcgat ccgcgaagcc ttccaggcct
     2101 acagcgccga cgtgcagagc ggcgccttcc ccggcccgga ccagaccatc gatgtcgatc
     2161 cggccgtctt cgccgcgctc gaagcggccc cggacggcac cccatcctcg gatcagccgg
     2221 ccaccgatac gccggacccg gaactgcgct atggcggcgc cggggccggg cccaacggct
     2281 aacatgcaaa ctttccgcac aatcggcgaa ctgcgcgcgg cgcgcgccgc cgtggggccg
     2341 ggcacgcgcg tcggcctcgt gcccaccatg ggctacctcc acgccgggca cctctcgctg
     2401 gtcgagcagg cgcgcaacga gtgcgacctg gtggtggtga gcatcttcgt caacccgacg
     2461 cagttcggcc ccacggaaga cctgagccgc tacccgcgcg acgaggcccg cgatctccaa
     2521 ttcctggagt cggctggcgt cgattgggtc ttcatccccg ccgccgagga gatctatcca
     2581 cccggcttcc aaacctatgt ggacgtgcgt gaggtaacga ccgtgctgga aggcgcggcc
     2641 cggcccggcc acttcgcggg cgtcgccacg gtcgtcgcca agctgttcaa cctggtgcag
     2701 cccggcgtgg cctatttcgg gcaaaaggac gcccagcagg ttgtcgtcat ccggcgcatg
     2761 gtgcgcgacc tgaacttccc ggtggacctg gtgatcggca tgaccatccg cgagcccgac
     2821 ggtctggcgc tgagttcgcg caacgtctac ctcgaccccg ccgagcggca ggcggcgctg
     2881 gtactgtccc gtgccttgcg agccgcgcat gacctctggg acgcgggcgc gcgcgacggc
     2941 gaacggctgc gcgccgccat gcgcgccgtg ctggccgccg agccgctggc ccaacccgac
     3001 tacgtcagca tcgccgaccc cgacacgctg gccgaactca acgccatccc gccgggccag
     3061 ggcgccctgg cctcgctcgc cgtgcgcatc gggcggggca cgcggctgat cgataatcat
     3121 attctggagt agctatcagc catcagctat cagccgtcag gttaagaacg atttgtaggg
     3181 cagtggtagt accgaacaga gcgaacacaa atccgagaat acctgatagc tgacggctga
     3241 tagctgacgg ctgacggctg acggctgacc actatgctgc tagcaatcga tgtcggcaat
     3301 accaatgccg tgatcgggat ttacgagggg gccacgctgc gcgcgtcctg gcgggcggcg
     3361 acggaccatg agcgcaccgc cgacgagtgg ggcgggctga tctatacctt cctgtcggcg
     3421 cgcggcatgg ccttgcgcga agtccacgcc tgcatcgtct ccagcgtggt gccgccggtc
     3481 acgtcggcgc tgcgcgaaat gagcgccggg ctgctgggcc acccggccat ggtcatcgag
     3541 ccgggcctca agacgggcgt gcgcatcgcc atcgacaacc cgcgcgaagt gggcgccgat
     3601 cgcatcgtca acgccgtcgc cgcctaccgc cgctacggcg ggccatgcat cgtggtcgac
     3661 ttcggcacgg cgacgacggt ggacgccatc ggcgcggacg gcgcgtattt aggcggggcc
     3721 atcgcgcccg gcctcaccac ctccgccgag gcgctctacc ggctggccgc ccgcctctac
     3781 tcagtcgagc ttgtggcccc gcgcagcgtg atcggcaaga acaccgtcaa cagcatgcag
     3841 gcgggcatcg tcatcggcta cgtcgcgctg gtcgaggggc tggtggggcg catgaaagcc
     3901 gaactccagg ccggcggcgc gcccgccgat ctgccggtca tcgccaccgg cggcctctcc
     3961 ggcctcatcg cccacgaaac ggccgtgatc acccacaccg accccgactt gacgctcgac
     4021 ggcctgcgca tgatctacga aatgaacgcc catcagcagg gggcagtatg agcgccttcc
     4081 aggacaaacg catcgtcctc ggcgtctgcg gcgggatcgc ggcctacaaa gtggccgacc
     4141 tggcgagcaa gctgacccag gccggggcgc aggtggacgt gatcatgacc gccgccgccc
     4201 agcgcttcgt cacgccgctg accttcgccg ccctcaccca ccgcgaggtg cgcaccgacc
     4261 tctatgaaag ctggctgggc gaggacaccg gccacgtggc cctggcccac caggcggcgg
     4321 ccctcgtcgt ggccccggcc acggcccaca ccatcgcgcg gctggcgctc ggcctcggcg
     4381 acgatttcct gagcgccgtc gccctggcga ccacggcgcc gctgctcatc gtgccggcca
     4441 tggaacataa catgtatcac cacccggcca cgcaaggcca cctggcgacg ctgcgcgagc
     4501 ggggcgcgct ggtgatggag ccgggcgcgg gccggctggc ctccggcgcc acgggcacgg
     4561 gccgcctgcc ggagacgccg cagatcctgg gcgcgctgcg cgtgctgctg ggccggggcg
     4621 ggccgctggc ggggcagcgc atcgtcatca ccgcgggcgg cacgcaggaa ccgctcgacc
     4681 cggtgcgcta tgtcggcaac cgctc
//
LOCUS       DASUYC010000178         4321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1217855, whole genome shotgun sequence.
ACCESSION   DASUYC010000178 DASUYC010000000
VERSION     DASUYC010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4321
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217855"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1659)
                     /locus_tag="VF276_02860"
     CDS             complement(<1..1659)
                     /locus_tag="VF276_02860"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6605834.1"
                     /translation="MTNRNLPPDPPGPPDDGQATRRYPTNDPNPNPPNPATPGDPNAA
                     GAAPGPAPWETGGTPAQPPRAPWETTGAQTPPPARAPWETTGSTPAGAAAPGTPWPPT
                     TPGAGTPGGPSPSDPTAVYAVPPTAGLPPEPPAAYPPAGANIVYSTPTEAAPPPPPVP
                     PPTGPVYPPPSQGPGPLPWIIGAVVALALLGLLVFLLVNNNRNPVVAPTPGPTATATE
                     TPLPSPTPATTATATPPGPTDTPSPAPPTETPSPAPTSTPVPGLPTVTPLPTSTPAPP
                     PTDTAVPATATSAPVPTTAVPAPGTGTTPTLPPLPVFTATPGTTPAPSDVTPGTTGTA
                     PAGGPNAPDTTPAASQEGYLTVQWFGQSSFLLAGGEGVRILIDPVGPNLGYHLPVFNN
                     LDALLISHLHQDHTYTQVVAGGTPNYVGLNTNGGFQPITQNVKTAAVRDVGSFHDDQN
                     GHQRGANAMWVIDMDGFHIVHLGDLGQTELDDKQLQQLGQPDILMIPVGGNFTIDGRQ
                     ARAIMAQLKPRLVIPMHYRTDRTPANLNLAPVDAFLGQAPPPNPP"
     gene            1804..2850
                     /gene="ruvB"
                     /locus_tag="VF276_02865"
     CDS             1804..2850
                     /gene="ruvB"
                     /locus_tag="VF276_02865"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX6605835.1"
                     /translation="MSDSGDRVISGRAGEAERVIEQNLRPKRLPEYIGQEKVKESLEI
                     AIAAAKARGEALDHILLHGPPGLGKTSLANVLANEMGAQLKTTSGPAIERPGDLASIL
                     TNMQKSEVLFIDEIHRLNRAVEEVLYPAMEDFALDIIIGKGPAARSLRLKLPRFTIIG
                     ATTRLAMLSSPLRDRFVAPYRLEFYTQTAMETIISRSAGILQVPIDAEGTTEIARRAR
                     GTPRIANRLLRRVRDYAQVRAGGSITGEVAIEALNRMEVDPLGLDETDRRILLTIIQK
                     FGGGPVGVATIAAATSEDPDTIEDVYEPYLIQLGFLQRTPRGREVTPAAYSHLGLSGP
                     GRKANSSLQSQLPL"
     gene            2966..3196
                     /locus_tag="VF276_02870"
     CDS             2966..3196
                     /locus_tag="VF276_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HEX6605836.1"
                     /translation="MDLSEMGKWLVLAGVGLALLGGLIWLLGRVPFLGRLPGDINIQV
                     GNVSCFAPIVTMILLSVILTIVLNVLLRLFQK"
     gene            3207..4316
                     /gene="queA"
                     /locus_tag="VF276_02875"
     CDS             3207..4316
                     /gene="queA"
                     /locus_tag="VF276_02875"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEX6605837.1"
                     /translation="MHTSDFDYHLPPELIAQTPAQPRDSSRLLVLPRDGGPIAHRRFT
                     DILSYFQPGDLLVANESRVLPARLLGHKAATGGHIEALLLRPAAEQPDAAPAAGGNLV
                     WEALVKPGRSLRTGTRVIFSSAAREGRPGPPAGVVLEAEIVGRTEIGGRLLRFDQPPE
                     PWLDRIGVMPLPPYIHEPLADPERYQTVYARVPGSAAAPTAGLHFTPRLITALREKGV
                     QWTTVTLHIGLDTFRPVQEDDPRTHPMHREWYELPAATAAAINATRAAGRRVVVVGTT
                     TVRVLETVAQEQGLGDAGNDTLRPAIGWSQLFIYPGFRFRVTDSLVTNFHLPRSTLVM
                     LVSALVGRERLLAAYEEAVREAYRFYSFGDAMLIL"
BASE COUNT          602 a         1452 c         1594 g          673 t
ORIGIN      
        1 gggcgggttg gggggtggcg cctggcccag gaaggcgtcc accggggcca ggttcaggtt
       61 agccggggtg cggtcggtcc ggtagtgcat ggggatgacc aggcgcggct tcaactgggc
      121 catgatggcg cgcgcctggc ggccgtcgat ggtgaagttg ccgcccaccg ggatcatcag
      181 gatatcgggc tggcccagtt gctgcaactg cttgtcgtcc agctcggtct gccccaggtc
      241 gcccaggtgc acgatgtgga agccgtccat gtcgatcacc cacatggcgt tggcgccccg
      301 ctggtggcca ttctggtcgt cgtggaacga gccgacatcg cgcacggcgg ccgtcttcac
      361 gttctgcgtg atcggctgga agccgccgtt ggtgttgaga cccacgtaat tcggcgtgcc
      421 cccggccacc acctgcgtgt acgtgtggtc ctggtgcagg tggctgatga gcagggcgtc
      481 caggttgttg aacaccggca ggtgatagcc caggttcggc cccaccgggt caatcaagat
      541 gcgcacgcct tcgcctccgg ccagcaggaa actggactgg ccgaaccatt gcaccgtcaa
      601 gtagccctcc tgcgaagcgg cgggcgtggt gtcgggcgca ttcggcccac cggcgggagc
      661 cgtgccggtg gtgcccggcg tgacatccga gggtgcgggc gttgtgccgg gtgtggcggt
      721 gaagaccggc agcggcggca gcgtcggcgt ggtgcctgtc cccggtgccg gcaccgcggt
      781 cgtgggcacg ggtgcggatg tcgcggtcgc gggcacggcg gtgtcggtgg gcggcggggc
      841 cggggtgctg gtcggcagcg gcgtcaccgt cggcaggccc ggcaccggcg tgctggtcgg
      901 cgcgggcgac ggcgtctcgg tcggcggcgc gggcgagggc gtatccgtcg ggcccggcgg
      961 cgtggccgtg gccgtggtgg ccggcgtggg actgggcagc ggcgtctccg tggccgtcgc
     1021 cgtggggccg ggcgtgggcg ccacgacagg gttgcgatta ttgttgacca gcaagaacac
     1081 gagcagaccc agcagtgcca gcgccaccac ggcgccgatg atccagggca gcggccccgg
     1141 cccctggctt ggcggcggat agaccggccc ggtcggcggc ggcaccgggg gcggcggggg
     1201 cgccgcctcg gtgggcgtgc tgtagacgat gttggccccg gcgggcgggt aggcggcggg
     1261 cggctcgggc ggtagcccgg cggtcggcgg caccgcgtag acggctgtcg ggtcggaagg
     1321 cgacggcccg cccggtgtgc ccgcgcccgg cgtggtcggg ggccacggcg tgccgggcgc
     1381 cgctgcgccc gcgggagtgc tgccggtcgt ctcccacggc gcgcgggcgg gcggcggcgt
     1441 ctgcgcgcca gtggtctccc agggggcgcg gggcggctgc gccggggtgc cgccggtttc
     1501 ccagggcgcg ggaccgggcg cggccccggc ggcgttcggg tcgccgggag tcgccgggtt
     1561 gggcggattc gggttcgggt cgttggtggg gtaccggcgc gtggcctgcc cgtcgtcggg
     1621 cggccccggc ggatccggcg gcaggttgcg attggtcatg gctggtcctc ctgtgccgca
     1681 gtagcgcggc tggctaaagc agcaaaatgc aactagcgtg ccatcctgcg gcgcgcgtgg
     1741 gcttgtggta taataagaca actagcaagt ttgttctaaa ggcaaggtga gggcaagcaa
     1801 gaagtgtcgg acagcggaga tcgggtcata tcaggccggg ccggcgaagc ggagcgggtc
     1861 attgagcaga acctgcgccc caagcgcctg ccggagtata tcggccagga gaaggtcaag
     1921 gagagcctgg agatcgccat cgccgcggcc aaggcgcgcg gggaggcgct cgaccacatc
     1981 ctgctgcatg ggccgcccgg cctgggcaag accagcctgg cgaacgtgct ggcgaacgag
     2041 atgggcgccc agctcaagac cacgtccggc ccggccatcg agcgccccgg cgacctcgcc
     2101 tcgatcctga ccaacatgca gaaaagcgaa gtcctgttca tcgacgagat ccatcgcctg
     2161 aaccgcgccg tggaagaggt gctctacccg gcgatggagg acttcgcgct tgacatcatc
     2221 atcggcaagg gcccggcggc gcgcagcctg cgcttgaagc tgccgcgctt caccatcatc
     2281 ggggcaacca cgcggctcgc catgctctcg tcgccgctgc gcgaccgctt cgtcgcgccc
     2341 taccgcctgg agttctacac gcagacggcc atggagacga tcatcagccg ctcggcgggc
     2401 atcttgcagg tgccgattga cgccgagggc acgaccgaga tcgcccgccg ggcgcgtggt
     2461 accccccgca tcgccaaccg gctcttgcgc cgcgtccgcg attatgccca ggtgcgcgcc
     2521 gggggcagca tcaccggcga ggtcgcgatc gaagccctga accgcatgga ggtcgatccg
     2581 ctcggcctgg acgagaccga ccggcgcatc ctgctgacga tcatccagaa gttcggcggc
     2641 ggtcccgtcg gcgtggcgac cattgccgct gcgaccagcg aggaccccga caccatcgag
     2701 gatgtctacg agccgtacct gatccagctc ggcttcttgc agcgcacacc gcgcgggcgc
     2761 gaggtgacgc ccgccgccta tagccatctc ggcctgagcg ggccgggccg caaggccaac
     2821 agcagcctcc agagccaact gccgctctga gtccgccacg gcacctgccc gccctcgttc
     2881 tatttagcgt ggaaggccga tgtaggggcc ggtttcaaac cggcccggtc gcaaatacgg
     2941 cggtggccta tcaggcgagg cggcgatgga tctgagcgag atgggcaaat ggctggtgct
     3001 ggccggggtg ggcctggccc tgctgggcgg cctgatctgg ctgctggggc gcgtgccgtt
     3061 cctggggcgg ctgcccggcg acatcaacat ccaggtgggc aacgtcagtt gcttcgcccc
     3121 catcgtcacc atgatcctgc tcagcgtgat cctgactatt gtgctcaacg tgctgttgcg
     3181 cctgttccag aagtaacctg atcttaatgc ataccagcga tttcgattat cacttaccgc
     3241 ccgaacttat cgcccagact ccggcccaac cgcgtgattc ctctcgcctg ctcgtgctac
     3301 ctcgcgacgg cggccccatc gcccaccggc gcttcaccga tattctgagc tacttccagc
     3361 cgggcgacct gctcgtggcg aacgagagcc gggtgctacc cgcccgcctg ctgggccaca
     3421 aggcggcgac cggcggccac atcgaggcgc tgctcttgcg ccccgccgct gaacagccgg
     3481 atgcggcccc ggctgccggg ggcaacctgg tgtgggaggc gctggtcaag ccgggccgca
     3541 gcctgcgcac gggcacgcgg gtcatctttt cgtcggcggc ccgagaaggg cggcccggcc
     3601 cgcctgcggg cgtggtgctg gaggccgaga tcgtcgggcg cacggagatc ggcgggcggc
     3661 tgctgcgctt cgaccagccg cccgaaccct ggctcgaccg catcggcgtg atgccgctgc
     3721 cgccctacat ccacgagccg ctggccgacc ccgagcgcta ccagacagtc tatgcgcgcg
     3781 tgcccggctc ggcggcggcg cccacggcgg ggctgcattt cacgccccgc cttatcaccg
     3841 ctctgcgcga gaagggcgtc cagtggacca cggtcacgct acacatcggc ctggacacct
     3901 tccgcccggt gcaggaggac gatccgcgca cccacccgat gcaccgcgag tggtatgaac
     3961 tgcccgcggc gacggcggcg gccatcaacg ccacgcgcgc cgccgggcgc cgggtggtcg
     4021 tcgtcggcac gaccaccgtg cgtgtgctgg agaccgtggc ccaggagcag gggctgggcg
     4081 atgccggcaa cgacacgctg cgcccggcca tcggctggag ccagttgttt atctatccag
     4141 gcttccgctt ccgcgtcacc gacagcctgg tgaccaactt ccacctgccg cggtccacgc
     4201 tggtcatgct ggtcagcgcg ctggtgggcc gcgagcgcct gctcgccgcg tatgaggaag
     4261 ccgtgcgcga ggcctaccgc ttttactcgt tcggcgacgc catgctcatc ctttagctgg
     4321 a
//
LOCUS       DASUYC010000179         2087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_326058,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000179 DASUYC010000000
VERSION     DASUYC010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326058"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VF276_02880"
     CDS             <1..583
                     /locus_tag="VF276_02880"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6605838.1"
                     /translation="EDEYKAYGYDFPSAGVRVDELEEAMQIIEALWTDEQATVHGQYY
                     QVTDAYCEPKPDPRPPIMIGGAKPRMLRVVARHADWWNVSWTGIEDYRRQVPECERAC
                     AEVGRDPRTLRRTWFGGCLCAPTEAAVQRLNTTGMSAANAFVGTPAQLIEQMSAFVEL
                     GVDYFMLGCGGFPELTTLEMLVHEVLPALNRGR"
     gene            725..895
                     /locus_tag="VF276_02885"
     CDS             725..895
                     /locus_tag="VF276_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605839.1"
                     /translation="MPPRGVKEGSKRDRQYEHIKDSYEDRGVSAKEAEERAARTVNKD
                     RREAGETRKDKD"
     gene            939..1076
                     /locus_tag="VF276_02890"
     CDS             939..1076
                     /locus_tag="VF276_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605840.1"
                     /translation="MSEQKCANCGMPKSEWQGNNGQGITLGDKTYCCQGCAIGTGCTC
                     K"
     gene            complement(1146..>2087)
                     /locus_tag="VF276_02895"
     CDS             complement(1146..>2087)
                     /locus_tag="VF276_02895"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6605841.1"
                     /translation="LYAELLAGQRDSYQVEQRYIRRDGSVLWARRTASLVRTEAGEPQ
                     FVISVIEDITTRKQAQEALAASLAAQQAANRQLAELNQAKSDFVSIVSHEFRTPLTVI
                     QGFSEMLRDEEIDPDEIKEYAGDINTEAVRLNRMINDLLDLERMESGRMTLHRQATDL
                     NGVIRDVVELMRPNAPGHTLAVDLDPALPIFEGDQDKLVQVLTNLLHNAVKYSPDGGE
                     VLVASRVEGPLAHVEVRDHGLGIPAEALETIFERYARIESAANRHIQGTGLGLPIVRQ
                     IVEMHGGRVWAESEVGAGAVFHVTIPLHGLPRNAGTA"
BASE COUNT          362 a          664 c          705 g          356 t
ORIGIN      
        1 ggaggacgaa tacaaagcct acggctacga cttccccagc gcgggcgtcc gcgtggacga
       61 gttggaagag gcgatgcaga ttatcgaggc gctctggact gacgagcagg ccacggtgca
      121 cggccaatat taccaggtga cggatgccta ctgcgaaccc aagcccgatc cgcggccccc
      181 gatcatgatc ggcggcgcca aaccgcgcat gttgcgcgtg gtcgctcgcc acgccgactg
      241 gtggaacgtt tcctggaccg gcatcgagga ctaccgccgg caggtgccgg agtgcgagcg
      301 tgcctgcgcc gaggtcgggc gcgacccgcg gacgctgcgc cgcacctggt tcggcggctg
      361 cctctgcgcg ccgaccgagg ccgccgtgca acggctcaat accacgggca tgtcggcggc
      421 caatgccttc gtcggcacgc ccgcgcaact gatcgagcag atgagcgcct ttgtggagtt
      481 gggggtcgac tacttcatgc tcggctgcgg cggcttcccg gagctgacca cgctggagat
      541 gctggtccac gaggtgctgc ccgcgctcaa tcgggggcgg tagggccaac atgccggtcc
      601 gcgcctatgg gatagcggca aagtagtgcg agatccggcg ctgccggcgc gggtggcttg
      661 gcctgctatt tgctctaaaa atgggagcga ccgcgttgtt tagcggggcg aaaggaggtc
      721 cagtatgcca cctagaggtg tcaaggaagg cagtaaacgc gatcgtcaat acgagcatat
      781 caaggactcc tatgaagatc gcggggtttc cgcaaaagaa gctgaggaac gggccgcgcg
      841 caccgtgaac aaggaccgcc gcgaagctgg agaaaccagg aaggacaagg actaagtacg
      901 ctgcggccgg tatggcgccg cggaaaggag caatccccat gtctgagcag aaatgcgcta
      961 attgtggcat gcccaagagc gagtggcaag gcaacaacgg ccagggcatt accctgggcg
     1021 ataagaccta ttgctgccag ggctgcgcta tcggcaccgg gtgtacctgc aagtaagccc
     1081 ggcgcgccca gggcgcaccc atttgcaaag aacggcgggc cgcccggccc gccgcttctt
     1141 gttttctagg ccgtgccggc gttgcggggc aggccgtgca gcgggatcgt gacgtggaag
     1201 accgccccgg cgcccacctc gctttcggcc cacacccgcc cgccgtgcat ctccacgatc
     1261 tggcgcacga tgggcagccc caggcccgtg ccctggatat gccggttggc cgccgattcg
     1321 atccgcgcgt agcgctcgaa aatcgtctcc agcgcctcgg cggggatacc cagcccatgg
     1381 tcgcgcactt cgacatgggc cagcggtccc tcgacgcggc tggcaaccaa cacctcgccg
     1441 ccgtccggtg agtacttgac cgcgttatgc agcaggttgg tcagcacctg caccagcttg
     1501 tcctggtcgc cctcgaagat gggcagcgcc gggtccagat ccacggccag cgtgtggccc
     1561 ggcgcgttgg ggcgcatgag ctcgaccacg tcgcggatca cgccgttgag atcggtcgcc
     1621 tgccggtgca gcgtcatgcg gcccgactcc atccgttcga ggtcgagcag gtcgttgatc
     1681 atgcggttga gccgcaccgc ttcggtgttg atgtcgccgg cgtattcctt gatttcgtcc
     1741 gggtcgattt cctcgtcgcg cagcatttcg ctgaagccct ggatcacggt gagcggggtg
     1801 cgaaactcat ggctgacaat cgacacgaaa tcgctcttgg cctggttgag ttcggccagt
     1861 tgccggttgg cggcctgttg ggccgccagc gaggcggcca gggcctcctg ggcctgcttg
     1921 cgcgtggtga tgtcctcgat cacgctgatg acgaactgcg gctcacccgc ttccgtgcgt
     1981 accagcgacg cggtgcgccg ggcccagagc acgctgccgt ccctgcggat atagcgctgc
     2041 tcgacctggt agctgtcgcg ctgcccggcg agcaactccg catagag
//
LOCUS       DASUYC010000180         3829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_318957,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000180 DASUYC010000000
VERSION     DASUYC010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318957"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="VF276_02900"
     CDS             complement(<1..937)
                     /locus_tag="VF276_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605842.1"
                     /translation="MRKMQMWLLACGAMLLLMGTLALGAPARANGLVHIQDDANVLSA
                     ADKQQITAAAQKAPFAVTVWTNSSYNTKDDFYNAVRGMVGNDTVVLGVDPVHKWSHIS
                     AKQDTGLTADQVNAAASQANAQFGNGAWAAGFTSALNNLAAVAPPGGTSGGTSGNTAP
                     AQQPAPASNGGGGFGLLACLIPIVLIVLAFFFLRNRMGGNRATAPMAPNQPYVPPQQG
                     GYPPQQGGYPPQQGGYYPQQGGYPQNRGSGIGGNIASGGLGALAGGLIGYELGRNAGE
                     DQGGQAGVQPGYAPDFGGGGGGIIDSASGGSGPDFG"
     gene            complement(1164..1895)
                     /locus_tag="VF276_02905"
     CDS             complement(1164..1895)
                     /locus_tag="VF276_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4261 domain-containing protein"
                     /protein_id="HEX6605843.1"
                     /translation="MSNPPKIVLGIPGLWQTRSDFVEAMALNSKGFVFAGRTLLNIAS
                     RASWELELRAYTTRLVEAFTVANRGSIDEPTMRAIAKHTFTAYIVGPGGSLDAARSIM
                     SAAVGVLHAGGLAVKVESAGVAHSSEQWKALTTATDAEALYYAYVTLVGQGDNRTYYS
                     CGMHNLGLRDAIITGNLTAYEAGSLLNTFLLYCLIEQPQLETGHTFSVDAQSPRYRLS
                     IEQCTVYPKDHLFNNPFGLWRLSPL"
     gene            complement(2036..3022)
                     /locus_tag="VF276_02910"
     CDS             complement(2036..3022)
                     /locus_tag="VF276_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605844.1"
                     /translation="MALNANEDTPKSSDPAGPVLSSPAPPAPDADGPSGITATETPPY
                     YSITPDGAAPSAANEAPVAPVDTVAPVDSTAPLPVRPAGRVAPPRPDFGAAHGENGAT
                     APAPAPWAVSNAPTPPPAQIYTPPPAPADTAPPPPADPAPAAPVLLTEAAPLEPLPAP
                     APAAPAPPPTGALVLLPGEEVIMQLGALYLTNKRAILYAPTILRAAFLRDVDAIGTVT
                     ERAGGWMLLFGLLAVALAGGAIYLGVTQPGSDLVLGDLFRANPFVVAVPLALLGLLML
                     ASYFFWVKRSLFLSVGGRPLIVVSISGWSGKKLESVDSFVNAFSQAKERERS"
     gene            3389..>3829
                     /locus_tag="VF276_02915"
     CDS             3389..>3829
                     /locus_tag="VF276_02915"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX6605845.1"
                     /translation="MSTSDSEAAARAGQVAALGAIIRAHIAAAGGRIPFADFMDLALY
                     HPEYGYYSAGTVTLGRGGDFTTSPEVDPAFGALLAEFVRRCDAALDHPPAFLLAEHGA
                     GSGRLLADLLDALRAGDPGLYARLDPVIVERSPALQARQLATLAA"
BASE COUNT          636 a         1277 c         1262 g          654 t
ORIGIN      
        1 ccccgaaatc gggaccgctg ccgccgctgg ccgagtcgat gatgccaccg cccccgccgc
       61 cgaagtcagg cgcgtagccg ggctgcaccc cggcctggcc gccctgatcc tccccggcgt
      121 tccggcccag ttcatagccg atcagcccac cggccagggc cccgaggccg ccggacgcga
      181 tgttgccgcc gatgccgctg ccccggttct gcggatagcc gccctgctgg ggatagtatc
      241 cgccctgctg cggcggatag cccccttgct gcggcggata gccgccctgc tgcggcggga
      301 cgtacggctg gttcggcgcc atgggcgcag tcgcccggtt gccgcccatg cggttgcgca
      361 ggaagaagaa cgccagcacg atcagcacga tcgggatcag gcaggccagc aggccaaacc
      421 cgcctccgcc gttcgacgcc ggggccggct gctgcgcggg cgccgtattc cccgacgtgc
      481 cgcccgacgt gccgcccggg ggcgccaccg ccgccaggtt gttcagcgcg ctcgtgaagc
      541 ccgcggccca ggccccgttg ccgaactgcg cgttggcctg gctcgccgcc gcgttcacct
      601 ggtccgccgt caggccggta tcctgcttgg cgctgatatg gctccactta tgcaccgggt
      661 ccacgcccag cacgaccgtg tcgttgccca ccatgccgcg cacggcgttg taaaagtcgt
      721 ccttggtgtt atagctgctg ttggtccaca cggtcacggc gaacggcgct ttctgggccg
      781 ccgccgtaat ctgctgcttg tccgccgccg acaggacatt ggcatcgtcc tggatatgca
      841 ccaggccgtt agcccgcgcc ggcgcgccga gcgccagcgt ccccatcagc agcagcatcg
      901 ccccacaggc gagcagccac atctgcatct tacgcatgaa tcagctcctt atgctagccg
      961 gcgtctccag gccagggggc gtctgcaacc ggtctacgct gcccggcccg cgccaccgcc
     1021 gggcgccttt ccctgctatt aaagcaaagg ctgtgccccg tcgcgccggc atccgcctat
     1081 acgcaaaaag gccgtggcgg ggtgccacgg cccttagttt atttcgatat cgtccctcta
     1141 tccgcatcag atttgcgtaa cagctataat ggcgataagc gccataagcc gaaaggattg
     1201 ttgaacaggt gatctttggg atagactgta cactgctcta tgctcaatcg atagcggggt
     1261 gattgcgcat cgacgctgaa tgtatgacct gtttctagct gcggttgttc gataaggcaa
     1321 tacagtagga aggtgttcag aaggctaccc gcctcatatg cggttaagtt gccggtgata
     1381 atggcatcac gtaaaccaag attatgcatt ccacaggaat aatatgtgcg gttatccccc
     1441 tgcccaacca gagttacgta agcataatac agtgcctccg cgtctgttgc ggtcgtcaac
     1501 gctttccatt gctcggaact atgagccacg ccggcagact cgaccttcac agccaggccg
     1561 cccgcatgaa ggaccccaac tgccgccgac atgatactcc gcgccgcatc aagggatccg
     1621 ccagggccga cgatgtaggc tgtgaaagta tgtttagcaa tcgcccgcat ggttggctca
     1681 tctattgaac cgcggttggc tacggtaaac gcctccacca gacgtgtggt atatgcgcgt
     1741 agctccagtt cccaggaggc tcgcgaagct atattcagca gagtccgacc tgcgaatacg
     1801 aaacccttgc tattcagcgc catggcttcc acaaaatcgg atcgagtttg ccacaaccca
     1861 ggaataccaa ggacgatctt cggaggattc gacatcttca caagctccga ggtacaaacg
     1921 gtcgtttcac tttacaatgc gatatcattc gtcatagccc tatcaacaaa gagcgaggcc
     1981 gcagtactta cactgcggcc tttcagcaca agttaattag ggccgattga tttcgctaag
     2041 accgctcgcg ctccttggcc tggctgaagg cgttgacgaa gctgtccacc gactccagct
     2101 ttttgccgct ccagccggag atgctgacca cgatcagcgg gcgcccgccg accgagagga
     2161 acaggctgcg cttgacccag aagaaatagc tcgccagcat gagcaggccg agcagggcca
     2221 gcggcacggc caccacgaag gggttggcac ggaagaggtc gcccagcacc aggtcgctgc
     2281 ccggctgggt cacgccgagg tagatggcgc caccggccag ggccacggcc agcaggccga
     2341 acagcagcat ccagccgccc gcgcgctcgg tcacggtgcc gatggcgtcc acatcgcgca
     2401 ggaaggcggc gcgcaggatg gtcggggcgt agaggatggc ccgcttgttg gtcaggtaga
     2461 gcgcgcccag ttgcatgatc acttcctcgc cgggcagcag caccagcgcg ccggtgggcg
     2521 gcggcgcggg ggcggcgggg gccggggcgg gcaacggctc cagcggcgcg gcctcggtga
     2581 gcaagaccgg ggcggcgggg gccggatcgg cgggcggggg cggcgcggtg tcggcgggcg
     2641 ccgggggcgg ggtgtagatc tgcgccgggg gcggcgtcgg ggcattgctc acagcccagg
     2701 gcgcgggcgc gggggccgtg gcgccgtttt cgccgtgcgc ggcgccgaag tcaggcctcg
     2761 gcggggccac gcgcccggcc gggcgcaccg gcaagggcgc cgtgctgtcc accggggcga
     2821 cggtgtcgac cggcgcgacc ggtgcctcgt tagcggcaga tggcgcggcg ccgtcgggcg
     2881 tgatgctata atacggcggc gtttcggttg ccgtgatgcc gctcggaccg tcggcgtcgg
     2941 gggccggcgg agcgggcgac gagaggacgg ggccggcggg atcgctgctc ttgggggtgt
     3001 cctcgttcgc gttcaaagcc atcgagtgtg tgaccttcct tagccaaatt tcgtactgat
     3061 gttatacggg cgcagtgata cagcggcatt atacccgccg atttgcggcc ctgtcaaccg
     3121 cgaggcgacg gcggcacccg tatcacgcgg tagctttctc cctttccgcg ccgggaagtg
     3181 gggccggggg tttagtgcag cttcaggcat cctgctgggg ataacgcacg ctgacgacaa
     3241 acgagtacgg gcgcgataaa tcgcgccgct acaaccaaga gccggctaga atctgcatca
     3301 cggcgtttcc cccttcccgc gccgggaaga ggggcagggg gttaggcccg tcgcggaact
     3361 gaggaccatg gaccacgtta gttatcatat gagcacttct gattccgagg ccgccgcgcg
     3421 ggccggccag gtcgccgcgc tgggggccat catccgcgcc cacatcgccg cggcgggcgg
     3481 gcgcattcct ttcgccgact tcatggacct ggcgctgtat cacccggagt acggctacta
     3541 cagcgcgggc accgtcacgc tgggccgggg cggcgacttc accacctcgc cggaggtgga
     3601 cccggccttc ggcgcgctgc tggccgagtt tgtgcgccgc tgcgacgccg cgctcgacca
     3661 cccgcccgcg ttcctgctgg ccgagcacgg cgccgggtcg ggccgcctgc tggccgacct
     3721 gctcgacgcc ctgcgcgccg gcgatcccgg cctctacgcc cggctggacc cggtcatcgt
     3781 cgagcgcagc ccggcgttgc aggcccgcca gcttgccacc ctggccgcg
//
LOCUS       DASUYC010000181         8344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1584452, whole genome shotgun sequence.
ACCESSION   DASUYC010000181 DASUYC010000000
VERSION     DASUYC010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 8344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8344
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1584452"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1722
                     /locus_tag="VF276_02920"
     CDS             <1..1722
                     /locus_tag="VF276_02920"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial transcriptional activator
                     domain-containing protein"
                     /protein_id="HEX6605846.1"
                     /translation="LREEGPGEDTLSVRVKLRTGQLDEAQRILESWAESERREAARGQ
                     MHQPRAHRETLLILSLIHAFRGEAGRAFTLAEEGIATGARLESPFITAVGHMRLGHAW
                     QVRGNDSWPPERAPQAGEAIRCYQQAIAQGDRLAVRRIRAEAMWGLTRAYGFAGDLEA
                     AERAAAEGVETGRWAGDSWIVALIELTLGASYVLAGRAGEGVEILTRVLVAFRDCGDR
                     FGRAATRLWLALAYHDLRRPELLATHLDDGLALCEAHGYDFLFTAPTLNGPPDPRRFV
                     PLLVAARARRRRAAYATRLLAALGLADIPAHPGYQLRVQTLGAFRVWRGDVEVEPREW
                     QRDKARQVFQVLLGERGGWLQREEIVERLWPTLSAEAAGRDFKVALNALNKALEPGRA
                     ADAPFAFIVREGTAYRIRPDADLWLDAVAFEDACEAGLRLAADTPGAALDPLRTAMRL
                     YAGDYLPDALYEDWASAERERLLALYLRAADRLTGILVERGAYEEALDICGRILARDP
                     CWERAYRWMMLAHARQGNRSPALRAYARCAQTLQAELGVDPSPATTALHESIVQARAL
                     PETRAVG"
     gene            1932..2663
                     /locus_tag="VF276_02925"
     CDS             1932..2663
                     /locus_tag="VF276_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020048974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX6605847.1"
                     /translation="MGWEALKQWGDDVARLAPLTGGVANDVWTVRANGHLAVARLGRR
                     SDADLAWETALLAHLDRAGLAVPVPIPTTDGRLFAAGVVVMTYVEGGPPETEADWRRV
                     AATLRRLHRLTAGWPQRPGWRSSTDLLHAATGTKIDLGAMPPEGVARCRAAWARLVGR
                     PTCVVHGDPNPRNIRLTAERVALIDWDEAHVDVPDLDLVLPYNAAGLDEEAHDIAAQA
                     SAAWEAAVCWDDQYSIKRLAEVRAV"
     gene            2909..3298
                     /locus_tag="VF276_02930"
     CDS             2909..3298
                     /locus_tag="VF276_02930"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX6605848.1"
                     /translation="MREPDADTVQQVIQAERAWLQAHLELDTALLDHLMAEEYTQVDS
                     HGALVGKQAVLASLRSGTREWDWADSDEYRVQIYGAVAVVYGRWRARGVNAGHPFDYA
                     ARYVSVWVYRDGRWQMVSDQSTEIGPE"
     gene            complement(3403..3624)
                     /locus_tag="VF276_02935"
     CDS             complement(3403..3624)
                     /locus_tag="VF276_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEX6605849.1"
                     /translation="MLDGTLIEQNADVLGGTPVFAGTRVPVHTLLDYLIAGDSLADFL
                     DDFPTVTRAQAIAVLELLKQILLAEFSLK"
     gene            complement(3677..4879)
                     /locus_tag="VF276_02940"
     CDS             complement(3677..4879)
                     /locus_tag="VF276_02940"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX6605850.1"
                     /translation="MTALDPTCHPRWARTVAELEPRPATAGGRAAISFAYGLPDPALF
                     PTEALNAATGIVLREHSDRALQYGAGQGLPQLINVLVERLNRAEGLAIRPDECMITAG
                     AGQAIAVVGRALLDPGDTVLVETPAWPGGINAFRRCGARLVPVPVDAEGLDTDALAQT
                     LETLAAAGTQPKLLYTVPNFQNPTGVTTTLARRQALLDLARRYDFVILEDDAYRDLAF
                     EGPLPPSLFALDTDGRVLRTGTYSKILAAGLRLGWLLGPRDVLPRLIALKDDGGTSPF
                     TSYLAATYSRAGELDPHIARLVASYRRKRDVMLAALTRYFPKSVTWTVPAGGFFVWVT
                     LPPGTDAADLLRAAREEGVDFLPGRSCFPDPQAGTSHLRLAFSLPTPDQIEEGIKRLG
                     NVLAAALG"
     gene            complement(4892..5803)
                     /locus_tag="VF276_02945"
     CDS             complement(4892..5803)
                     /locus_tag="VF276_02945"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX6605851.1"
                     /translation="MDFRTELNNIISDEHPFSVAALTFLSDISSADAATLALMWPGAG
                     VARRRRIAATLVELAEDNIEYDFSNVFNVLLTDPDAEVRRSAVEGLVEDESIGTVRRL
                     IDVLDSDPAPDVRAAAALVLGPAALRAETGKLKGEWPARLCDALLRAVRDPAAGEDVH
                     RRALESVAYFCDNADVAAEIERAYAGQGLLPTSAVNAMGRNMNTRWAPTLLKELQSPN
                     PAMRYEAAHAVGELGERANVPALIPLLDDADLEVQLAAIWALGQLGGRVATQALQLRA
                     QDDDEAIREAVDEALTEIRYASDPLQP"
     gene            complement(6082..8130)
                     /locus_tag="VF276_02950"
     CDS             complement(6082..8130)
                     /locus_tag="VF276_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605852.1"
                     /translation="MNSATVAAKGGPAFEGTPAQQELAAKAFDLMRRQGMLFAADAPI
                     VVTLDRLVQGLSRSYPKVAADKLKDQVTAALDANPGVFTRQEVKGAVAYETTKSGQRP
                     ADMTDARHMFRHRLNDAAREVTEQESRSLTEGWIAQAQARAESFTIFQEPAAQAAVSG
                     QWQGPADIAAFVAPTTPAPNAAPMPQPVSPMTPWPQAVPEAEPIAASDVDESVLIDTP
                     EAPIVPESEELPLEVTPAAPPPPAQVEPVAAAPVVEPAAPALAPATPPAPVAPVAPAA
                     PPAPVAPTRPVRYTLPTEAGEATIDLAPGVDGALEEQGAALEAMLAQALRDDFRFASF
                     GALWYPEEHVERFSKGDFRRIKEYLIETQEALSDRAFLNDVLSRRENDPDYERLRFSL
                     NYRMLREKKDFEFVGVPDDHLWIVAGASPVSVPKRKPSELGQDYRFLEDAAIRALEPE
                     VPADLTQAEHALTYYEYENGLLPYDNAMRVLLPRPMLDEQRAALLRIEVPALFQSFPV
                     ELRYPTGNRGGYLVGFDAFFQENLVPGATFTLESTEQQNVLLLRFERTSAQEASLLQY
                     DERRNKFVYRPTTYYAGTSPDWLLSEKRFPNLDGVKRLDEAERKKTENVVTRAFELVG
                     ETVDGRLFALTSDLLPVVNLERPFSPGALRAVLETGNHSYFEADPDTPGAFFYDPSKK
                     "
BASE COUNT         1169 a         2838 c         3093 g         1244 t
ORIGIN      
        1 ctgcgcgagg aaggccccgg cgaggacacg ctgagcgtgc gcgtcaaact gcgcaccggc
       61 caactggacg aggcccagcg catcctggaa tcgtgggcgg agagcgagcg gcgcgaggcg
      121 gcgcgcgggc agatgcacca gccgcgggcg catcgcgaga cgctgctgat cctgtcgctg
      181 atccacgcct tccgcggcga ggccgggcgc gccttcaccc tggccgagga aggcatcgcc
      241 accggggcgc ggctggagtc gccgttcatc accgccgtgg gccacatgcg gctgggccac
      301 gcctggcagg tgcgcggcaa cgactcctgg ccgccggagc gcgcgccgca ggccggcgag
      361 gccatccgct gctaccagca ggcgattgcc cagggcgacc ggctggccgt gcgccgcatc
      421 cgcgccgagg cgatgtgggg cctcacccgc gcctacggct tcgcgggcga cctggaggcg
      481 gcggaacggg cggccgccga gggcgtggag acgggccgct gggccggcga ctcctggatc
      541 gtggcgctga tcgagctgac cctgggcgcg agctacgtgc tggccgggcg ggccggcgag
      601 ggggtcgaga tcctgacccg cgtgctggtc gccttccgcg actgcggcga ccgcttcggg
      661 cgggcggcca cgcggctctg gctggccctg gcctaccacg acctgcgccg ccccgaactg
      721 ctggcgacgc acctggacga cgggctggcg ctgtgcgagg cgcacggtta cgacttcctg
      781 ttcaccgcgc ccacgctcaa cggcccgccc gatccgcgcc ggttcgtgcc gctgctggtg
      841 gcggcgcgcg ccaggcgccg ccgcgccgcg tatgccacgc gcctgctggc cgcgctgggg
      901 ttggccgaca tccctgccca ccccggctac cagttgcgcg tgcagacgct gggcgccttc
      961 cgcgtctggc gcggcgacgt tgaagtcgag ccgcgcgagt ggcagcgcga taaggcgcgg
     1021 caggtgtttc aggtgctgct gggcgagcgc ggcggctggc tgcaacgcga ggagatcgtc
     1081 gagcgtctgt ggcccaccct gagcgcggag gccgccgggc gcgacttcaa ggtggcgctc
     1141 aacgcgctga acaaggcgct cgaaccgggc cgcgccgccg acgcgccgtt tgccttcatc
     1201 gtccgcgagg gcacggccta ccgcatccgc cccgacgccg acctgtggct cgacgccgtg
     1261 gccttcgagg acgcctgcga ggcggggctg cggctggccg ccgatacgcc gggcgcggcg
     1321 ctggaccccc tgcgcacggc catgcgcctc tacgcgggcg actacctgcc cgacgcgctc
     1381 tacgaggact gggccagcgc cgagcgcgag cggctgctgg cgctctacct gcgcgccgcc
     1441 gaccggctca ccggcatcct ggtcgagcgc ggcgcctacg aggaagcgct ggacatctgc
     1501 gggcgcatcc tggcccgcga cccctgctgg gaacgcgcct accgctggat gatgctggcc
     1561 cacgcccgcc agggcaaccg ctcgccggcc ctgcgcgcct acgcccgctg cgcgcaaacg
     1621 ctgcaagccg aactcggcgt ggacccctcc ccggccacca cggccctcca cgagtccatc
     1681 gtccaggccc gtgccctgcc cgaaacgcgg gcggtgggct agcgcgcgcc gggaagcatg
     1741 atttcgcgct gtaggggccg gtttcaaacc ggcccgcgcc cgtccaattg cgctgtaggg
     1801 gccggtttca aaccggcctg ccgcccgtca gaatcgggct gggggatcac caccgccccc
     1861 ggcgaaggac agcattacac ttgtggcgcc agggcgtgat ccggcgctgc gggaagggga
     1921 ggatgaggcc cgtgggatgg gaggcactca agcagtgggg tgacgatgtc gcgcgcctcg
     1981 caccgctcac cggcggcgta gccaacgacg tgtggaccgt gcgcgccaac gggcacctcg
     2041 cggtcgcgcg gctcggtcgc aggagcgacg cggatcttgc ctgggagacc gcgctcctcg
     2101 cccacctcga ccgggccggg ttggccgtgc cggtgccgat cccgacgacg gacggccggc
     2161 tgttcgccgc cggggtggtg gtgatgacct acgtcgaggg cggcccgccc gagacggagg
     2221 ccgactggcg gcgcgtggcc gccacgctgc gccggctgca tcggttgacg gcggggtggc
     2281 cgcagcgccc cggctggcga tcatcgaccg atctgctgca cgccgcgacc gggacgaaga
     2341 tcgacctcgg cgcgatgccg ccggagggcg tggcgcgctg ccgcgccgcc tgggcgcggc
     2401 tcgtgggccg gccgacctgc gtggtccatg gcgaccccaa cccgcgcaac atccgcctga
     2461 ccgccgagcg ggtcgcgctg atcgactggg acgaggcgca cgtcgatgtc cccgacctcg
     2521 acctggtgct gccctacaac gctgccgggc ttgacgagga ggcgcacgac atcgcggcgc
     2581 aagcgtcggc cgcctgggaa gccgccgtct gctgggacga ccagtactca atcaagcggc
     2641 tggccgaagt gcgcgcggtc tgagccgccg cggccacgaa tccccagacc gctcgctgcg
     2701 aaccggcggg ctattgcgct gtaggggccg gtttcaaacc ggcccgcgcc cgtccaattg
     2761 cgctgtaggg gccggtttca aaccgacctg ccgccgtcgg gatcaatgcc gatattgcgg
     2821 tgtaggggcc ggtttcaaac cggcctgcca cccatcagaa tcgggctggg ggatcaccac
     2881 cgcccccggg agagaggata gcaccctgat gcgcgaaccc gacgcggata ccgtgcagca
     2941 agtcatccag gcggaaagag cctggttgca ggcacacctg gaactggaca cggcgctgct
     3001 cgatcacctg atggccgagg aatacaccca ggtggacagc cacggcgcgc tcgtgggcaa
     3061 gcaagcagtg ctggcgtcct tgcggagcgg cacgcgggag tgggattggg ccgacagcga
     3121 cgagtaccgg gtgcagatct acggcgcggt ggcggtggtg tacgggcgct ggcgcgcccg
     3181 cggcgtgaac gcgggccatc ccttcgacta cgccgcccgc tacgtctcgg tctgggtcta
     3241 ccgcgacggt cgctggcaga tggtgagcga ccagtctacg gagatcgggc cggagtaaac
     3301 ccactgtcca actagtgcag cgtcaagcat cctgttaggg acaaaacacg ctggcgacaa
     3361 acgagtacgg gcgcgatcaa tcgcgccgct acgcccatga ggtcacttga ggctgaattc
     3421 ggccagcaaa atctgcttga gcaattccag cacggctatt gcctgcgcgc gggtgacggt
     3481 ggggaagtcg tccaggaagt cggcgaggga gtcgcccgcg atcaggtaat cgagcagggt
     3541 gtgcaccggc acgcgggtgc cggcgaagac cggcgtgccg cctaacacgt ccgcgttttg
     3601 ttcaatcagc gtgccgtcca gcatggctgt tgcctccacg cattatcccg gcccgatggg
     3661 cgaggagcgt gcccgactag cccagcgcgg cggccagcac gttgccgagg cgcttgatgc
     3721 cttcctcgat ctggtcgggg gtgggcaggc tgaaggcgag gcgcaggtgg gacgtgccgg
     3781 cctgcgggtc ggggaagcag gagcggcccg gcaggaaatc aacgccctcc tcgcgggccg
     3841 cgcgcagcag atcggcggcg tcggtgccgg gcggcagcgt gacccagacg aagaagccgc
     3901 ccgccgggac cgtccaggtg acggacttcg ggaagtagcg ggtcagcgcg gccagcatta
     3961 cgtcgcgctt gcggcggtag ctcgccacca gccgggcgat atgaggatcg agttcgccgg
     4021 cccggctgta agtcgcggcc aggtaggacg tgaaggggct ggtgccgccg tcgtccttga
     4081 gcgcgatgag gcggggcagc acgtcgcgcg ggccgagcag ccagcccagg cgcagaccgg
     4141 cggccaggat cttcgagtag gtgcccgtgc gcagcacgcg cccgtcggtg tcgagcgcga
     4201 acagcgaggg cggcagcgga ccctcgaagg ccaggtcgcg gtaggcgtcg tcttccagga
     4261 tcacgaaatc gtagcgccgg gccaggtcga gcagggcctg ccgccgggcc agcgtcgtgg
     4321 tcacgccggt ggggttctgg aagttgggca cggtgtagag cagcttgggc tgtgtgcccg
     4381 cggcggccag cgtctccagc gtctgcgcca gcgcgtccgt gtccaggccc tcggcgtcca
     4441 ccggcacggg gacgaggcgc gcaccgcagc ggcggaaggc attgatcccg ccgggccagg
     4501 ccggggtctc gaccagcacc gtatcgccgg ggtccagcag cgcccggccc acgacggcaa
     4561 tcgcctggcc cgcgcccgcc gtgatcatgc actcgtcggg ccggatcgcc agcccttcgg
     4621 cgcggttgag ccgttcgacc agcacgttga tcaactgggg caggccctgg cccgcgccgt
     4681 attgcagcgc gcggtcgctg tgttcgcgca gcacgatgcc ggtggcggcg ttgagcgcct
     4741 cggtggggaa cagggcgggg tcgggcaggc cgtaggcgaa gctgatcgcc gcgcggcccc
     4801 cggcggtcgc cgggcgcggt tcgagttcgg ccacggtgcg ggcccagcgc gggtggcagg
     4861 tggggtcaag cgcggtcatg gcgcctcctg gtcagggttg cagcgggtcg gatgcgtagc
     4921 ggatctcggt cagggcttcg tccaccgcct cgcggatggc ctcgtcgtcg tcctgggcgc
     4981 gcagttgcag cgcctgggtg gcgacgcgtc cgcccagttg gcccagcgcc cagatggcgg
     5041 ccaactgcac ttccaggtcg gcgtcgtcga gcagggggat gagcgcgggc acgttggcgc
     5101 gctcgcccag ttcgcccacc gcgtgcgccg cctcgtagcg catcgccggg ttcgggcttt
     5161 gcagctcctt gagcagcgtc ggcgcccagc gcgtgttcat gttgcggccc atggcgttga
     5221 ccgcgctggt gggcagcagc ccctggcccg cgtaggcgcg ctcgatctcg gcggccacgt
     5281 cggcgttgtc gcagaagtag gcgacggact ccagggcgcg ccggtggaca tcctcgccgg
     5341 ccgcggggtc gcgcacggcg cgcagcagcg cgtcgcacag gcgggccggc cactcgccct
     5401 tgagcttgcc cgtttcggcg cgcagggccg ccggccccag cacgagggcg gcggcggcgc
     5461 gcacgtcggg cgccgggtcg gagtccagca cgtcgatcag gcggcgcacc gtgccgatgc
     5521 tctcgtcctc caccaggccc tcgacggcgc tgcggcgcac ctcggcgtcc gggtcggtca
     5581 gcagcacgtt gaacacgttg ctgaagtcgt actcgatgtt gtcttcggcc agttcgacca
     5641 gagtggcggc gatgcggcgg cggcgggcca cgccggctcc cggccacatc agggccaggg
     5701 tcgcggcatc ggcgctcgaa atgtccgaca ggaaggtcag cgcggccacc gaaaacgggt
     5761 gctcgtcgct gatgatgttg ttgagttcgg tgcgaaagtc catacgagct attagctgtc
     5821 agccgtcagc cgtcaggagt tacggtgacg ttggcggcta ttatgagcca tcagcggtca
     5881 gccgtcagcc gtcgggagtt accgggacgt tggcggctct gttgagttgt cagccgtcag
     5941 ccgtcagccg tcaggaacta cggagacgtt ggcggctctg ttgagttgtc agccgtcagc
     6001 cgtcagccgt cagccgtcag cccggcagcc cggatcactc ctgacggctg atagctgata
     6061 gctgatagct gacggctcgt cctacttctt gctcgggtcg tagaagaacg cgccgggcgt
     6121 gtcggggtcg gcctcgaagt acgagtggtt gcccgtttcg agcacggcgc gcagggcgcc
     6181 cggcgagaag ggccgctcca ggttgaccac gggcagcagg tccgaggtca gcgcgaacag
     6241 gcggccatcc acggtctcgc ccaccagctc gaaggcgcgc gtgacgacat tctcggtctt
     6301 cttgcgctcg gcctcgtcca gccgcttgac gccgtcgagg ttcgggaacc gcttctcgct
     6361 cagcagccaa tcggggctgg tgcccgcgta gtaggtggtg ggccggtaga cgaacttgtt
     6421 gcggcgctcg tcgtattgca gcagcgaggc ttcctgcgcc gaggtgcgct cgaagcgcag
     6481 cagcagcacg ttctgctgct cggtgctctc cagcgtgaag gtcgcgcccg gcaccaggtt
     6541 ctcctggaag aaggcgtcga agccgaccag gtagccgccg cggttgcccg tcgggtagcg
     6601 caactcgacc gggaagctct ggaagagcgc cggcacttcg atgcgcagca gggcggcgcg
     6661 ctgctcgtcg agcatgggcc ggggcaacag cacgcgcatg gcgttgtcgt agggcagcag
     6721 gccgttctcg tattcgtaat aggtcagcgc gtgctccgcc tgggtcaggt cggcgggcac
     6781 ttcgggttcc agcgcgcgga tggcggcgtc ttccaggaag cgatagtcct ggccgagctc
     6841 gctcggcttg cgcttgggca cgctgacggg cgacgccccg gccacgatcc agagatggtc
     6901 gtcgggcacg cccacgaact cgaagtcctt cttctcgcgc aacatgcggt agttcagcga
     6961 gaagcgcagg cgctcgtagt cggggtcgtt ctcgcggcgg ctcagcacgt cgttgaggaa
     7021 ggcgcggtcg gacagggctt cctgggtctc gatcaggtat tccttgatgc ggcggaagtc
     7081 gcccttgctg aagcgctcga cgtgctcttc gggataccac agcgcgccga aggacgcgaa
     7141 gcggaaatcg tcgcgcagcg cctgggccag catcgcctcc agggcggcgc cctgctcctc
     7201 cagcgcgccg tccacgccgg gcgcgaggtc gatggtcgcc tcgccggcct cggtgggcag
     7261 ggtgtagcgc acggggcggg tcggggccac gggggccggc ggggccgcgg gcgcgaccgg
     7321 cgcgaccggg gccggcgggg tcgcgggcgc gagggccggc gctgccggtt cgaccaccgg
     7381 ggccgcggcc acgggttcca cctgcgcggg cggcggcggg gccgcgggcg tgacctccag
     7441 cggcagttct tcggactccg gcactatcgg ggcttccggc gtgtcgatca ggacgctttc
     7501 gtccacatcg ctggcggcaa tcggctcggc ctcgggcacg gcctggggcc agggcgtcat
     7561 gggcgagacg ggctgcggca tcggcgcggc gttgggcgcg ggcgtggtcg gggcgacaaa
     7621 ggcggcaata tcggcggggc cctgccactg gccggacacc gcagcttgcg ccgccggctc
     7681 ctggaagatg gtgaacgact cggcgcgcgc ctgggcctgg gcgatccagc cctcggtcag
     7741 cgagcgcgac tcctgctcgg tcacttcgcg ggcggcgtcg ttgagccggt ggcggaacat
     7801 gtggcgggca tcggtcatgt ccgccgggcg ctgcccgctc ttggtggtct cataggcgac
     7861 ggcgcccttg acctcctgcc gggtaaacac accggggttg gcgtcgagcg ccgcggtgac
     7921 ctggtccttc aacttgtcgg cggccacctt gggatagctg cggctcaggc cctgcaccag
     7981 gcggtcgagc gtgaccacaa tcggcgcgtc cgccgcaaac aacatcccct ggcggcgcat
     8041 caggtcgaag gctttggcgg ccagttcctg ctgtgcgggc gtgccttcaa aggccggccc
     8101 cccttttgct gcgacggtag cgctgttcat gctgctcctt tatccttggt catgtcgtgt
     8161 agtcaaagcc gcgccttaac gaccgcgcgt cgcctcatcg cgcaagcgtt ccaaccagaa
     8221 ccgcagcagt tcctcggtcg tgcgcgggcg cccgctgcgc cgcatccatt ccaacaaccg
     8281 atccaggtcc tgcgggcgaa tacgcagttc ttcttccacc agtgtacccc tctcccatcc
     8341 cgat
//
LOCUS       DASUYC010000182         2289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_56995,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000182 DASUYC010000000
VERSION     DASUYC010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56995"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1247)
                     /locus_tag="VF276_02955"
     CDS             complement(<1..1247)
                     /locus_tag="VF276_02955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6605853.1"
                     /translation="MRLAYFSPLNPVQSGISDYSEELLPFLARGATVDLFIDKYKPSN
                     PAIPVQFRVLPAGRFDAAPYDSAVYQMGNSPAHAYIYERLLKTPGVLVLHEFVLHHLR
                     MWMALRRGKGRAYLQAMRATYGEAGRQAAERVLLGQHPEALFNFPLVEEALTRATGVI
                     VHSRYMADQVRQVRADVPLAVVNMGVPLPPYVPRDVARARLGLDPDVFILSSMGHLNP
                     YKRLNVALRAFKALVMQYPRALFLLVGSRSPNYNVDRMIAMLGLQERVRVIGYAPPAE
                     FADYLAASDVCINLRYPTAGETSAALLRIMGGGKPVLVSAVGSFTELPDAAAAKVDVG
                     GIEEELLLEDLLLLARRPDLRLAMGRAARRYVAEQHTLEGAARGYLTFLADLLGQPAP
                     VAGLVPPPYQDDMPTAPDPPFAPP"
     gene            complement(1247..2287)
                     /locus_tag="VF276_02960"
     CDS             complement(1247..2287)
                     /locus_tag="VF276_02960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6605854.1"
                     /translation="MAVCTAQVPFVHGGNEVLVEGLVAALRGRGHRVAEIALPFKWYP
                     RESFMGSVLAWRMIDITEANGVPVDLAICTKFPSYVVQHPRKVVWLVHQYRQAYDWFG
                     TPLSDLTSTPDDTRFRRWLADLDRKTIGEARGIYAISRNVAGRLRKYNGLQATPLYPP
                     LRPGFHPGPLGDYVLHLNRLDAAKRVDLLLRAAALVPRDAANGLRYVIAGDGPDRAAL
                     ERQAASLGLGERVRFAGRVSDAEAADLYAGARAVYYAPVDEDYGFSTVEAFASGKPVI
                     TTSDAGGVLEFVEDGVTGLVTPPDPARVAESLAQVAADPAACVRMGEAGRARVAGITW
                     DHVVDTLLAGAR"
BASE COUNT          348 a          808 c          831 g          302 t
ORIGIN      
        1 ggcggggcga agggcgggtc gggtgccgtg ggcatgtcgt cctggtaggg cggggggacc
       61 aagccggcga ccggcgcggg ttggccgagc agatcggcca ggaaggtcag gtagccgcgg
      121 gccgcgcctt cgagcgtgtg ctgctcggcc acgtagcggc gggccgcccg gcccatcgcc
      181 agtcgcagat cggggcgccg ggccagcagc agcaggtctt ccagcagcaa ctcctcctcg
      241 atcccgccca cgtccacctt ggccgccgcc gcgtccggca actcggtgaa cgagccgacc
      301 gccgagacca gcaccggctt gcccccgccc atgatgcgca gcagcgccgc cgaggtctcg
      361 cccgccgtgg ggtagcgcag gttgatgcag acatccgagg cggccaggta gtcggcgaac
      421 tcggcgggcg gcgcgtagcc gatgacgcgc acgcgctctt gcaggccgag catggcgatc
      481 atgcggtcta cgttgtagtt gggcgaccgg ctgccgacca gcaggaacag cgcccgcggg
      541 tactgcatga ccagcgcctt gaacgcgcgc agggccacgt tgagccgctt gtacgggttg
      601 aggtggccca tcgaggagag gataaacacg tcggggtcga ggcccagccg ggcgcgggcc
      661 acgtcgcgcg gcacgtaggg cggcagcggc acgcccatat tgacgacggc cagcggcaca
      721 tcggcgcgca cctggcgcac ctggtcggcc atgtagcggc tgtggacgat cacgcccgtg
      781 gcgcgggtca gcgcctcttc caccagcggg aagttgaaca gcgcctccgg gtgctggccc
      841 agcagcacgc gctcggcggc ctgccgcccg gcctcgccgt aggtcgcgcg catggcctgg
      901 agataggccc gccccttgcc ccggcgcagc gccatccaca tgcgcaagtg gtgcagcacg
      961 aactcatgca gcaccagcac gccgggcgtc ttcagcaggc gctcgtagat gtaggcatgg
     1021 gcggggctgt tgcccatctg gtagacggcg ctgtcgtagg gcgcggcgtc gaagcggccc
     1081 gccggcaaca cccggaactg caccgggatg gccgggttgg acggcttgta cttgtcgatg
     1141 aacagatcga ccgtggcccc acgcgccagg aacggcagca actcctcgct gtagtccgag
     1201 atgcccgact gcaccgggtt gagcgggctg aaataggcca gccgcatcag cgcgcccccg
     1261 ccagcagcgt atcgaccaca tggtcccagg tgatgcccgc cacgcgcgcc cgcccggcct
     1321 cgcccatgcg cacgcacgcc gccgggtccg cggctacctg cgccagcgac tcggctaccc
     1381 gcgccgggtc gggcggcgtg accagcccgg tcacgccgtc ctcgacgaat tcgagcacgc
     1441 ccccggcgtc gctggtcgtg atcaccggct tgcccgacgc gaacgcttcg acggtggaga
     1501 agccgtaatc ttcgtccacc ggggcgtaat agacggcgcg ggcgcccgcg tagagatcgg
     1561 cggcctcggc gtcggagacg cgcccggcga agcgcacccg ctcgcccagg cccaggcttg
     1621 ccgcctgccg ctccagcgcc gcgcggtccg gcccgtcgcc ggcgatgacg tagcgcaggc
     1681 cgttcgccgc gtcgcgcggc accagggcgg cggcgcgcaa gagcaggtcc acgcgcttgg
     1741 ccgcgtcgag ccggttcagg tgcagcacgt agtcgcccag cgggccgggg tggaagccgg
     1801 ggcgtaacgg cgggtagaga ggtgtggcct ggaggccgtt gtacttgcgc agccgcccgg
     1861 ccacgttgcg cgaaatggcg tagatgccgc gcgcctcgcc gatggtcttg cggtccagat
     1921 cggccagcca gcggcggaag cgggtgtcgt cgggcgtgga ggtgaggtcg ctcagcggcg
     1981 tgccgaacca gtcatacgcc tggcggtact ggtgcaccag ccagaccacc ttgcgcgggt
     2041 gctgcacgac ataggacggg aacttggtgc agatcgccag gtccaccggc acgccgttgg
     2101 cctcggtgat gtcgatcatg cgccaggcca gcacgctgcc catgaacgac tcgcgcgggt
     2161 accacttgaa cggcagcgcg atctcggcga cacggtgccc gcgcccgcgc aacgccgcca
     2221 ccagcccctc gaccagcacc tcgttgccgc cgtgcacaaa gggcacctgc gccgtgcaga
     2281 cggcaatgt
//
LOCUS       DASUYC010000183         2220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_655965,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000183 DASUYC010000000
VERSION     DASUYC010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655965"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..720)
                     /locus_tag="VF276_02965"
     CDS             complement(121..720)
                     /locus_tag="VF276_02965"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX6605855.1"
                     /translation="MQQPDVGDTSPRETQRANILEAARRVFARKGKAATMADVAQAAG
                     VSQGLAYRYFAGKEQIYRELVEQAVLQAGLATAPPPAESSATPGRRLALLITRLVEYR
                     RDHLEIFQLLDQVLNADQAQDEVTEEIRRRGERFVAELRQLIVEGQATGEVGSGDPDQ
                     LVIAIIAMLDGLVRFGLHHPEQFQRLCPDANIILRLLMP"
     gene            978..1616
                     /locus_tag="VF276_02970"
     CDS             978..1616
                     /locus_tag="VF276_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605856.1"
                     /translation="MPRPTRGRVLVLACAGLAGLLLWAGTRAPVTAPRLVTPGAAPHL
                     AAASSSPAPAPVSSQAPAAPRLGPSADIVDSADIIDSLDPTLPAPSPTDPPRLPPVPT
                     SLPPPAPAPAPAVDPYRRWIHEARLQYPYPESEEQMYQLLLCESSGNPAADSPDGLYH
                     GLFQYSAETWGGDWNPYRDQSIYDAQAQIWATARAWNLGMQQQWGCYSTLVP"
     gene            1893..>2220
                     /locus_tag="VF276_02975"
     CDS             1893..>2220
                     /locus_tag="VF276_02975"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEX6605857.1"
                     /translation="MDTSRTCPRCGNTLQPNDKFCKNCGMAVDAAPTQAMNAPEEAAR
                     ASYPPPGAGYQPPAPGGPQGYQPPPAPGGPQGYQPPAASGAGYQPPAPGPQDYQPPPG
                     GSQTYQP"
BASE COUNT          365 a          823 c          682 g          350 t
ORIGIN      
        1 gctgttgcct gggcgcgggc cgcgcggcgg cgggccggcg cgaaatctcg aatgtgctag
       61 cgcgtcgccg gcgcgccacg aactaccgcc tgggagcatg cccgcacgcg ggagccggtg
      121 ctacggcatg agcaggcgca ggatgatgtt cgcatcaggg cagagccgct ggaattgctc
      181 ggggtggtgc aagccgaatc tgactaggcc gtccagcatt gcgataatgg cgataaccaa
      241 ttggtcggga tcgccgcttc cgacttctcc tgtagcctga ccctcgacga tcagttgccg
      301 caactccgcc acaaagcgct cgccacgtcg gcggatctct tccgtgacct cgtcctgcgc
      361 ctgatccgcg ttgagcacct gatcgagaag ctggaagatt tccagatgat cgcgccggta
      421 ttcgaccagc cgggtaatca gcagggccag gcgccggccc ggtgtcgcgg aactctcggc
      481 tgggggcggc gcggtcgcaa ggccggcctg cagcaccgct tgctccacca gctcgcgata
      541 aatctgctcc ttgccggcga agtaacgata ggccagcccc tggctgaccc ccgccgcctg
      601 ggccacatcg gccatcgtgg ccgccttgcc cttgcgcgca aagacccgcc gggcggcttc
      661 gagaatattg gcccgctgcg tttcgcgggg actcgtatcc ccaacatccg gttgctgcat
      721 cgcagactcc tgctgagtga atgaatgatt cattcattaa tataaccgct cagtccctcc
      781 atgtcaagcg gcaaagctgg agatgaggga gaaaatggcg tccacgccaa cgctgtgcgg
      841 ccattcggac gctatcccag gccggccgtc cagcccgccc ggtgcccggc gggataagcc
      901 cgagttcgct tgcttgcatc cattggcgct gctatactgc gcgcatgcct cgccgttccc
      961 tgccccgccg cagatcggtg ccgcgcccta cgcggggccg cgtcctggtc ctggcctgcg
     1021 cggggctggc tgggttgctc ctatgggccg gcacccgcgc cccggtcacg gcgccgcggc
     1081 tcgttacgcc gggggctgcg ccccacctgg ccgccgcgtc cagctccccc gcccctgccc
     1141 cagtctccag ccaggcgccc gccgccccgc gcctggggcc atccgcagac atcgtggatt
     1201 cggcggacat catcgattcg ctggacccaa ccctgcccgc gccctcaccg accgatccgc
     1261 cacgcctccc gcctgtgccg acctccctac cgccgcccgc gcctgcgcct gcgccggcgg
     1321 ttgacccgta ccgccggtgg attcacgagg cccgcctgca gtatccttac cccgagagcg
     1381 aagagcagat gtaccagttg ctgctctgcg agtcgagcgg caaccccgcc gccgacagcc
     1441 ccgatgggct gtatcatggg ctattccagt acagcgccga gacctggggc ggcgactgga
     1501 acccctaccg cgaccagagc atctacgacg cgcaggccca gatctgggcc acggcccgcg
     1561 cctggaacct gggcatgcaa cagcagtggg gatgctactc caccctcgtc ccgtagcgcg
     1621 actcctacag ccggcgcgtc gaaatccggc taccctacgc agtaacatgc cccgcaattc
     1681 cgcaataaat acagcagcca tcctgcggcg cccggcgcgg ggccgcgcgg aaagccgccc
     1741 ggcggtgccc cggtgataca ggatgccggc accgcatcgg tccgtcccga caaaacggac
     1801 ttgtcatttg ctacaaagct tggtaatatg ccttgacccc tgatagggtc atcttttcgc
     1861 aatacgcgaa tcagctatta agtggaggat tcatggacac atctcgtacc tgcccgcgct
     1921 gtggcaatac gctgcaaccc aacgacaagt tctgcaagaa ttgcggcatg gcggtggatg
     1981 cagcgcccac ccaggcgatg aacgcaccgg aagaggctgc gcgggcctcc tatccgccgc
     2041 ccggcgccgg ctaccagccc cccgcgcccg gtgggccgca aggctaccag ccgccccccg
     2101 cgccgggcgg cccgcagggt taccagccgc ccgcggcgag cggcgccggc taccagccgc
     2161 ccgcgcccgg cccgcaagac taccagccgc cgcccggcgg gtcgcagacc taccagcccg
//
LOCUS       DASUYC010000184         3659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1056813, whole genome shotgun sequence.
ACCESSION   DASUYC010000184 DASUYC010000000
VERSION     DASUYC010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056813"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3659
                     /locus_tag="VF276_02980"
     CDS             <1..>3659
                     /locus_tag="VF276_02980"
                     /inference="COORDINATES: protein motif:HMM:NF018449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HEX6605858.1"
                     /translation="SVQLTLTPRIADRSTTPAEYTVRLSFVGADPTTRIDSGALLPGR
                     VNYLLGSDPAAWKTDLPTYGDVHYTALYPGVDLTYTGTEGRLKGTYTLAPGTDPTRIR
                     WRYSGAEALTLDAAGNLQITAAGGACDGAIVTEARPRAWQEIAGRRVAVPARYDLAAD
                     GTVGFALGAYDRNAPLTVDPYLSYATYFGGIIADLPQSLARGPDGSIYITGYTASSNF
                     PVVNAYQPTYQGQNDAFVARFTADGTPIYSTYLGGNYLDAATGIAVDSAGNIYVTGWT
                     GSTNFPTANAFQPTYAGNWDVFVTKLNPSGNALVFSTFLGGSAQENYQSTGDIAVDSA
                     GNVYVDGDTQSTNFPTHNAYQPQHSNPGSTDLFVTKFDPTGQALVYSTYLGGSNGGEY
                     SHGIATDAAGNTYVTGETTATNYPTRSAYQPACAPSFAGCWDVVLTKFNPSGSALVYS
                     TYLGGNDQEYVDRPYDVAVDPTGNAYITGFTGSPNFPVLNAYQVAYGGQVDVFVSAFS
                     TAGSLLYSTFLGGNNADVGGALALDPSGSFYVGGQTLSRNFPLLDPIQATLRGFEDGF
                     VTKFNAGGQTLGYSTYLGGSNNREEYGVVGIATDGAGGVTLVSSSSATDFPVVNAYQP
                     NNAGSYDGIIAQIQEGTPPTATGTPPTPTATPTGSPTPLPPSLLYSTYYGAWERDSIQ
                     DIGRDAAGNIYVMGTTFQQDIDYGDIYVAKFSPDGQTRLYQTILGGTRIDYGYGLSVD
                     AAGNATAVGIATSFDYPVVNAIQPNHALGIYDGVVTKLNSSGSIIFSTYLGGSGTDYA
                     QRVATDATGNVYIVGNTSSTNFPTTAGVFQPSNRGGVDGYITKITANGSAIAWSTYLG
                     GVYVDEIHDIALDGTGSLYLTGFTASPDYPLLNAFQRTYFDGEAFVTKMNPQGTALVY
                     STYLGGNNPPGPGEDNGQGIVVDATGAAYVTGYTQSPNFPVTAGAFQTVFRGYYDAFV
                     TKFTPAGNAVVYSTFVGGTVNPPEGDDEAFDIAVDGAGQAHITGKTNSPDFPVVRAVQ
                     PMKADVYDGFVTKLNASGSDLVYSTFLGGHYDPPDATGEEAGEAILIDGAGNAVIGGG
                     TGSFDFPIANAYQPQNAGESDGFISKLSGSNPLPTSTPTAVVARTATATAPPAGTPRP
                     ATPSATAPAAPPTRPPAASATPAEATATPCAISFSDVYPTDYFYEAVRYLYCHGVISG
                     YADGTFRPYNETTRS"
BASE COUNT          658 a         1342 c         1095 g          564 t
ORIGIN      
        1 tccgtccaat tgaccctgac cccgcgcatc gccgatcgca gcaccacgcc tgcggagtac
       61 accgtgcgcc tcagcttcgt cggcgccgat ccgacgacgc ggatcgacag cggcgccctc
      121 ttgcccggcc gagtcaacta cctactgggc agcgacccgg ccgcctggaa gacggacctg
      181 ccgacctacg gtgacgtcca ctacacggca ctctaccccg gcgtggatct gacctacacc
      241 ggcaccgagg ggcggctgaa aggaacgtac accctcgccc ccggcaccga tccaacccgg
      301 atccgctggc gctacagcgg cgcggaggcg ctcacactcg acgccgcggg caatctgcaa
      361 atcacggcgg ccggcggcgc gtgcgacggc gcgatcgtga ccgaggcgcg cccgcgggcc
      421 tggcaggaga tcgccggccg gcgcgtagct gtgcccgcgc gctatgatct cgctgccgac
      481 ggcacggtcg gctttgccct tggcgcttat gaccggaacg cccccctgac tgtcgatccc
      541 tacctgagct acgcgactta ttttggtggg atcatcgcgg acctgccgca gagtctcgcg
      601 cgcgggccgg atggcagcat ctacatcacc ggctacaccg cgtccagcaa cttcccggtc
      661 gtcaacgcct accagccgac ctaccagggc cagaacgacg ccttcgtcgc ccggttcacc
      721 gccgacggca ccccgatcta ttccacctat ctcggcggga actacctcga cgccgcgacg
      781 ggcatcgctg tcgacagcgc cggcaacatc tacgtcaccg gctggaccgg ctcgaccaac
      841 ttcccgacgg ccaacgcctt ccaacccacc tacgcgggca actgggatgt gtttgtcacc
      901 aagctgaacc ccagcggcaa cgcgctggtc ttctcgacct ttctgggggg cagcgcccag
      961 gaaaactacc agtccaccgg cgacatcgcc gtggactcgg cggggaacgt ctacgtcgat
     1021 ggggatacgc agtccaccaa cttccccacg cacaatgcct accagccgca gcactccaat
     1081 cccggcagca cggacctgtt cgtcaccaag ttcgacccga ccggccaggc tctggtttat
     1141 tcgacctacc tggggggcag caacggcggc gagtactcgc atgggatcgc gaccgatgcc
     1201 gccggcaaca cctacgtgac cggggaaacc acggccacga actatcctac gcgcagcgcg
     1261 tatcaaccag cctgcgctcc aagcttcgcc ggctgctggg acgtcgtgct gaccaagttc
     1321 aatccctccg gctcggcgct ggtctattcc acctatctgg gcggcaacga ccaggaatat
     1381 gtggacaggc cctatgatgt ggccgtggac ccgaccggca acgcctatat cacgggcttc
     1441 accggctccc ccaatttccc cgtgctcaac gcctaccagg tcgcctatgg gggccaggtc
     1501 gacgtgttcg tcagcgcatt cagcaccgcc gggagcctgc tctattcgac tttcctgggc
     1561 ggcaacaacg cggatgtcgg cggggcgctg gccctggacc catcgggcag cttctacgtc
     1621 ggcggtcaga ccctgtcgcg caacttcccg ctgctcgatc cgatccaggc gaccctgcgc
     1681 ggattcgaag acgggtttgt gaccaagttc aatgccgggg ggcagacgct cggctactcg
     1741 acctacctgg gcggcagcaa caaccgcgag gagtatggcg tggtcggcat cgccacggac
     1801 ggtgcgggcg gcgtaaccct ggtcagtagc tcctcggcca ccgatttccc ggtggtcaat
     1861 gcctaccagc cgaacaacgc gggcagctac gacgggatca tcgcccagat ccaggaaggg
     1921 accccgccga cggcgacggg cacgccgcca acccccacgg cgaccccgac cggcagcccc
     1981 accccgctgc cgccgagcct cctctactcg acctactacg gcgcgtggga gcgcgattcg
     2041 atccaggaca tcggtaggga cgccgcgggg aacatctatg tgatgggcac caccttccag
     2101 caggacattg actacggcga catttacgtc gccaagttct cccccgacgg ccaaacgcgc
     2161 ctgtaccaga ccatcctggg cggcacgcgc atcgactatg gctacgggct gagcgtcgac
     2221 gcggcaggca acgcgacggc ggtgggcatc gcgacctcgt ttgactaccc cgtggtgaat
     2281 gccatccagc ccaaccatgc cctgggcatc tatgacgggg tcgtaaccaa gctcaattcc
     2341 agtgggagca tcatcttctc gacctatctg ggtgggagcg gcactgatta cgcccagcgc
     2401 gtcgccaccg acgcgacggg caacgtctac atcgtcggta acaccagctc caccaacttc
     2461 ccgaccacgg caggcgtctt ccagccgtcc aatcggggcg gcgtcgatgg gtacatcacc
     2521 aagatcaccg ccaacggctc ggcgattgcc tggtccacct acttaggcgg ggtgtacgtc
     2581 gacgagattc acgacatcgc cctggatggg acgggcagcc tctacctgac cgggtttacc
     2641 gcgtccccgg actatccgct gttgaacgcc ttccagcgca cctacttcga cggcgaagcc
     2701 ttcgtcacca agatgaaccc gcagggcacg gcgctcgtct actccaccta cctggggggc
     2761 aataatcccc cggggccggg cgaggacaac ggccagggca tcgtcgtgga cgcgacgggg
     2821 gcggcctatg tcaccggcta cacccaatcg cccaacttcc ccgtcaccgc cggggcgttc
     2881 cagaccgtct tccggggcta ctacgacgcc tttgtgacta agttcacccc ggccggcaac
     2941 gcggtcgtct actcgacttt tgtcggcggc acggtcaacc cgccggaggg cgacgacgaa
     3001 gctttcgaca tcgccgtaga cggggccggt caggcccaca tcacaggcaa gaccaactcc
     3061 cccgacttcc ccgtcgtgcg tgcggtccag ccgatgaagg cggacgtgta cgatgggttc
     3121 gtgaccaagc tgaacgccag tggttccgac ctcgtgtact cgacgttcct gggcgggcac
     3181 tacgatccgc cggacgccac cggggaggaa gcgggcgagg ctatcctgat cgatggcgcg
     3241 gggaacgcgg tgatcggggg gggcaccggc tcgttcgact tccccatcgc caacgcctac
     3301 cagccgcaga acgcggggga gagcgacggg tttatcagca agctctcagg cagcaacccc
     3361 ctgcccacta gcacgcccac agccgttgtc gcgcgcacgg ccactgctac cgcgccgccc
     3421 gccggcaccc cgcgcccggc gacgccctcg gccacggcgc ccgccgcgcc cccgacccgc
     3481 ccccccgcgg cgagcgccac gccggcggag gccacggcga cgccctgcgc gatcagcttc
     3541 agcgatgtct atccgacaga ctacttctac gaggcggtgc ggtatctcta ctgccacggg
     3601 gtgatcagcg gctacgcgga cggcaccttc cgcccgtaca acgagaccac gcgcagcca
//
LOCUS       DASUYC010000185         3218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1266079, whole genome shotgun sequence.
ACCESSION   DASUYC010000185 DASUYC010000000
VERSION     DASUYC010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266079"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VF276_02985"
     CDS             complement(<1..471)
                     /locus_tag="VF276_02985"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:NF014857.4"
                     /note="catalyzes the formation of
                     N(1)-(5-phospho-D-ribosyl)glycinamide from
                     5-phospho-D-ribosylamine and glycine in purine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX6605859.1"
                     /translation="MIRVLVIGSGAREHALVLALARSPQVERIYCAPGNPGTALVAEN
                     LAQTADSEAAIQELAKWAFEQQIDLTVVGPELPLTLGVVDEFRAVGLPIVGPARAAAQ
                     LESSKSWARAFMDRHGIPAPQYQVTYTAEELGAYLAHARFPLVLKVDGLAGGKGT"
     gene            complement(536..>3218)
                     /locus_tag="VF276_02990"
     CDS             complement(536..>3218)
                     /locus_tag="VF276_02990"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEX6605860.1"
                     /translation="DSVLQFEPWLVYSAARLHAGALPLWNSDNMLGAPFIGNMQSAVF
                     YPLNWPYLLWPDGSMLALRAWLKLFVAALGMYLLAREVVRVGPVGAALAAATFTFGGF
                     LIAWLLYPLTGAVIWLPWLWWATGRLMAQPGARAVAALALVVALTLLAGQPEMAFHMT
                     AATGLLALFLAWQAAPRRVGPLLRRLGLWAAAFVLGALPAAIQIVPFLEYLAHSAALA
                     SRSGSGPAQFWLPFYLAWTAISPDLFGNPAHGTWWDTWTNYNEANSYSGIVPLVLAPF
                     AFLAPDRRQRALAAFLLGGSVIAAGVVYHWPGVFDVVTALPLLRQAANQRLLVVIECA
                     LGILAALGVCGLRARLPAWRRRLLLGLGLWTGVLLAVGIGVPLLLAYSFFRLPPDGPA
                     VGVWQAALGRGAVLVLVSSALVGGIFALWGARPRAARALLVALPLLLVADLWQARIDY
                     TPTVAPGQYFPPTAATRFLQQQPGPFHTVAAGWMLPPNTNLAYGLADLRGYDAVEPRT
                     YHDLAVALDPSIYQGPGGGFKPFNAIQSPLIDLLNVRYVLAPPGTDPNPTTLVDVRQE
                     GPPDRPVGEIFGTYRPGQTFTAGLDNLTAVEVFGATFGRPHGGRLIFHLKTDPAAPGD
                     LATVTVDAATLKDDSWWTFSFPPIAQARGKQFYFYFEAPDVPERQSVTVYYTQRDPYA
                     GGTRMAGDQPQDGDLVFRTLAGPDPADPWFARVLDGGPDGTSVFANRRALPRAWLVHR
                     AEVVPDDKTRLARLADPAFDRAGTALLDAPPAAPLDLPATAPPLGSDTATITRYAPED
                     VEIRTESPAAGLLILADQAFPGWEARVDGQPVPILTADHALRAVAVPGGAHTVRFTYA
                     PGSFGLGAGLTGLALVLVAGLGWAPRWRPFPLPGNRL"
BASE COUNT          521 a         1175 c         1138 g          384 t
ORIGIN      
        1 ggtgcccttg ccgcccgcca ggccgtcgac cttgagcacc agcgggaaac gcgcatgggc
       61 caggtaggcg cccagttcct cggcggtgta ggtcacctgg tattgcggcg cggggatgcc
      121 gtggcggtcc atgaaggccc gtgcccacga cttgctcgac tccagttgcg cggcggcgcg
      181 cgccggcccg acaatcggca ggcccacggc gcggaactcg tccacgacgc cgagcgtcag
      241 cggtagctcc ggcccgacga cggtcaggtc aatctgctgc tcgaaggccc acttggcaag
      301 ctcctgtatt gccgcttcgc tgtcggcggt ctgcgccagg ttctcggcca ccagcgccgt
      361 gccggggttg ccgggcgcgc aatagatgcg ctccacctgc gggctgcgcg ccagggccag
      421 caccagcgca tgttcccgcg ccccgctgcc aatcaccagc acccgtatca cctttggact
      481 ccttcccaga cccacccggt tagtcgcatc atctctatca gggtgtctcg ctgcactata
      541 gccgattgcc cggcaaaggg aaaggccgcc accggggtgc ccagcccagc ccggcgacca
      601 gcaccagcgc cagccccgtg aggcccgcgc caagcccgaa tgaaccgggt gcataggtaa
      661 agcggacggt gtgcgcgccg cccggtaccg ccaccgcgcg caacgcgtgg tcggcggtca
      721 ggatgggcac cggctggccg tccacacgcg cctcccagcc ggggaacgcc tggtcggcca
      781 ggatgagcag gccggcagcg gggctttcag tgcggatctc cacgtcctcc ggcgcgtagc
      841 gcgtgatcgt cgccgtgtcg ctgcccagcg gaggggcggt ggcaggcaga tccagcggcg
      901 cggcgggcgg ggcgtcgagc agcgcggtcc cggcccggtc gaaggcggga tcggctagcc
      961 gggccagccg cgtcttgtcg tcaggcacca cctcggcccg gtggaccagc caggcgcggg
     1021 gcaacgcgcg ccggttggcg aagacgctcg tgccgtccgg tccgccgtcc agcacgcgcg
     1081 caaaccacgg atcggcgggg tcgggaccgg ctagcgtacg gaagaccagg tcgccatcct
     1141 gcggctgatc tccggccatg cgtgtgccgc cggcataggg gtcccgctgc gtgtaataga
     1201 ccgtcaccga ctgccgttcg ggcacatccg gcgcctcgaa gtaaaagtag aactgcttgc
     1261 cgcgcgcctg ggcgatgggc gggaaggaga acgtccacca gctatcgtcc ttcagcgtgg
     1321 cggcatccac cgtcaccgtc gccaggtcgc ccggcgcggc gggatctgtc ttgaggtgga
     1381 agatcaagcg ccccccgtgc gggcgcccaa aagtcgcgcc gaagacctcg accgcggtca
     1441 ggttatccag ccccgccgtg aaggtctggc cgggccggta ggtgccgaag atctcgccca
     1501 cggggcggtc cggcggcccc tcctgccgca cgtcgaccag cgtggtcggg ttggggtcgg
     1561 tgcccggcgg cgccaggaca tagcgcacgt tcagcaggtc gatcagcggg gattggatgg
     1621 cgttgaacgg cttgaagccg ccgcccggcc cctggtagat cgacgggtcg agcgccacgg
     1681 cgagatcgtg gtaggtgcgc ggctccacgg cgtcatagcc gcgcagatcg gccagcccgt
     1741 aggccaggtt ggtattgggc ggcagcatcc acccggcggc caccgtgtgg aacggccccg
     1801 gctgctgctg caaaaagcgc gtcgccgccg tgggcgggaa gtactggccc ggcgccacgg
     1861 taggcgtgta gtcgatgcgc gcctgccaga gatcggcgac cagcagcagc ggcaaggcaa
     1921 ccagcagggc gcgcgccgcc cgtggccggg cgccccaaag ggcaaagatc ccgcccacca
     1981 gcgcgctgct caccagcacc agcacggccc cgcgccccag cgccgcctgc cagacgccca
     2041 ccgcggggcc atccggcggc agccggaaga agctatacgc cagcagcagc ggcacgccga
     2101 tgcctaccgc cagcagcacg ccggtccaga ggccgaggcc cagcagcagc cgccggcgcc
     2161 acgcgggcag ccgcgcccgc agaccgcaga cgccgagcgc ggccaggatg cccagggcgc
     2221 actcaatgac cacgagcagc cgctggttgg ccgcctggcg cagcaacggc agcgccgtca
     2281 ccacgtcgaa gacacctggc cagtggtaga ccacgcccgc cgcgatgacg ctgcccccca
     2341 gcaggaacgc agccagcgcg cgctgccgcc gatccggcgc caggaaggcg aagggcgcca
     2401 gcaccagggg cacgatgccg ctgtagctgt tggcctcgtt gtagttggtc caggtgtccc
     2461 accaggtgcc gtgggccggg ttgccgaaca ggtcggggct gatcgccgtc caggccaggt
     2521 agaacggcag ccagaactgc gccgggcccg atccgctgcg gctggccagc gcggcgctgt
     2581 gcgccaggta ctccaggaag ggcacgatct ggatggccgc cggcagcgcg cccagcacaa
     2641 aggcggcggc ccatagccct agccgccgca gcagcggccc cacccggcgc ggcgccgcct
     2701 gccaagccag gaacagggcc agcagccctg tcgccgcggt catgtggaag gccatctccg
     2761 gctgcccggc cagcagggtc agcgcgacca cgagcgccag ggcggccacc gcccgcgcgc
     2821 ccggctgggc catcagccgc ccggtggccc accacagcca gggcagccag atcaccgcgc
     2881 cggtcagcgg gtagagcagc cacgcgatga ggaacccgcc gaaggtgaag gtggccgccg
     2941 ccagcgccgc cccaaccggc ccgacgcgca ccacctcccg cgccagcagg tacatgccca
     3001 gcgcggccac aaacagcttc agccaggcgc gcagggccag catactgccg tcgggccaga
     3061 gcaggtaggg ccagttgagc gggtagaaca ccgccgactg catgttgccg atgaacggcg
     3121 cgccaagcat gttgtcggag ttccagagcg gcagcgcccc cgcgtgcagc cgggcggcgc
     3181 tgtagacgag ccacggctcg aattgcaaca cgctgtcg
//
LOCUS       DASUYC010000186         2792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_518577,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000186 DASUYC010000000
VERSION     DASUYC010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518577"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="VF276_02995"
     CDS             complement(<1..803)
                     /locus_tag="VF276_02995"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEX6605861.1"
                     /translation="MAAPESPIALVVHGGAGNIPDDEVAAYRAGVQAAALAGWAVLRG
                     GGSALDAIEAAIRLMEDDPTFDAGRGSFLNRDGVVELDAGIMHGPTLDLGMVAGVRGV
                     ANPITLARRVMESGRFVMLVGEGAAEYARASGLPLAADADLIVPRERERWEALRRDPT
                     FDVATMFTPPTSGGTVGAVALDRAGQVVAGTSTGGSQFKHPGRVGDSPIVGAGFYADA
                     GGGASSTGHGELILKVGLARRAVEALAPPAGLDPPAAAAAALARLAALPG"
     gene            complement(803..2644)
                     /locus_tag="VF276_03000"
     CDS             complement(803..2644)
                     /locus_tag="VF276_03000"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX6605862.1"
                     /translation="MKTRPGFLRPRLTPGLIAAVQQVGAAQWAEGDYVIYQRSHDKRA
                     AFERVPLDVGPVLRLTSDPPPAGAAAYTGALFDVSEDHVVYTATDGGLALMPLAGGPG
                     RRLATIAGTRALAPTFAPGGARLAFVTDNGQTADVGLIDVREGAWPRIIPAEADFVID
                     PTWHPDETHLAWVEWDVPNMGWDESRIVLCDVATGAQRAVLAGAGVSYSQPRWSPDGR
                     RLAFLCDKTGYLNLWLADADGGNPRPLVEEALEHGTPPWGSGVRTFDWTPDSTCIVLG
                     RNLAGSWGIRVVEVASGAGRDLPVAPGVYTGLRCSPDGGYLLAGFSGPATAPQIVTYN
                     LQTGARRVLATGAVGGVEDGAVAPQAITWQAPDGLTVPGLLYQPALRVQERAPLLVWV
                     HGGPTGQSNATFQPMIQYFVQRGWAVLMPNHRGSAGYGRAFTVAERGEWGGADMADIL
                     AGVDAVIARGGIDAGRVVPIGGSAGGYAVLQLLVLYPDRFKAGVDLFGISDHLELART
                     THRLEARYLDQLLGPLPESAALYKERSPIFHLDRIQAPLLILQGAEDRAVPVAQAQRI
                     YDGLRARGQTVELQIYPGEGHGWQQAATVADALTRIDDFLRTYGLLR"
BASE COUNT          406 a         1039 c          996 g          351 t
ORIGIN      
        1 ccgggcagcg cggcaaggcg cgccagggcc gcagcggcag cagcgggcgg atcaagtccg
       61 gcgggcggag ccagcgcctc cacggcgcgg cgcgccaggc ccaccttgag gatgagttcg
      121 ccgtggccgg tgctcgacgc cccaccgccc gcgtcggcgt agaacccggc gccgacgatg
      181 ggcgagtcgc ccacgcgacc ggggtgcttg aactggctgc cgccggtgga cgtgcccgcc
      241 accacctggc ccgcgcggtc gagggccaca gcgccgacgg tgccgcccga ggtgggcggg
      301 gtgaacatgg tcgccacgtc gaaggtgggg tcgcgacgca gcgcctccca gcgctcccgc
      361 tcgcgcggca cgatcaggtc ggcatcagcg gccagcggta ggccgctcgc gcgggcgtac
      421 tccgccgcgc cctcgccgac cagcatcacg aagcgcccgc tctccatcac gcgccgggcc
      481 agggtgatcg gattcgccac gccgcgcacg cccgccacca tgcccaggtc gagcgtcggc
      541 ccgtgcatga tgcccgcgtc cagttccacc acgccgtcgc ggttcagaaa actgccgcgc
      601 ccggcgtcga aggtgggatc gtcctccatc agccggatcg ccgcctcgat cgcatccagg
      661 gcgctgccgc cgccgcgcag cacggcccac ccggcgagag ccgccgcctg cacgcccgcg
      721 cggtaggcgg ccacctcgtc gtcggggatg ttgcccgcgc cgccgtggac gaccagcgcg
      781 atgggcgatt cgggggccgc catcaacgga gcagcccgta ggtgcgcagg aaatcgtcga
      841 tgcgggtgag cgcatcggcc accgtggccg cctgctgcca gccgtggccc tcgccggggt
      901 agatctgcaa ctccacggtc tgcccgcgcg cccgcaaccc gtcgtagatc cgttgcgcct
      961 gggccaccgg caccgcccga tcctcggcgc cctgcaagat gagcagcggc gcctggatgc
     1021 ggtccaggtg gaagatcggc gagcgttcct tatagagcgc ggcgctttcg ggcaacgggc
     1081 cgagcagctg gtcgagataa cgggcttcga gccggtgcgt ggtgcgcgcc agttcgaggt
     1141 ggtccgagat gccgaacagg tcgacgcccg ccttgaagcg gtcggggtag agcaccagca
     1201 actgcaacac ggcatagccg cccgcgctgc cgccgatggg caccacgcgc ccggcgtcga
     1261 tcccgccccg cgcgatcacg gcgtccaccc cggccaggat gtcggccatg tcggcgccgc
     1321 cccactcgcc ccgttcggcc acggtgaagg cgcgaccata gcccgcgctg ccgcggtgat
     1381 tgggcatcag cacggcccag ccgcgctgca cgaaatactg gatcatcggc tggaacgtgg
     1441 cgttggattg cccggtcggg ccgccgtgga cccagacgag caggggcgcg cgctcttgca
     1501 cccgcaacgc gggctggtac agcaaccccg gcacggtcag gccatcgggt gcctgccagg
     1561 tgatcgcctg cggcgccacg gcgccatcct ctacgccgcc caccgccccg gtcgccagca
     1621 cacgccgggc gcccgtctgc aaattgtacg tcacgatctg cggcgccgtg gccgggccgc
     1681 tgaacccggc tagcaagtag ccgccgtcgg gcgagcagcg caggccggtg tagacgcccg
     1741 gcgccaccgg gagatcgcgc cctgccccgc ttgccacctc gaccacgcgg atgccccaac
     1801 tgcccgccag gttgcgcccc agcacgatgc aggtgctgtc gggcgtccaa tcgaaggtgc
     1861 gcacgcccga cccccagggc ggcgtgccgt gctcgagcgc ctcttcgacc agcgggcgcg
     1921 ggttgccgcc gtcggcatcg gccagccaga ggttgaggta gccggtcttg tcgcacagga
     1981 aagccagccg gcggccgtcg ggcgaccagc gcggctggct gtagctcacg cccgccccgg
     2041 ccagcaccgc ccgctgcgcc cccgtggcca catcgcacag gacgatgcgg ctctcgtccc
     2101 agcccatgtt gggcacgtcc cactcgaccc aggccaggtg cgtctcgtcg gggtgccagg
     2161 tggggtcgat gacaaagtcg gcctcggcgg ggatgatgcg cggccaggcg ccttcgcgta
     2221 cgtcgatcag cccgacatcc gccgtctggc cgttgtcggt gacgaaggcg aggcgggcgc
     2281 cgccgggagc gaaggtgggg gccagcgcgc gggtgccggc gatggtcgcc aggcggcggc
     2341 ccggcccacc ggccagcggc atcagcgcca gcccgccgtc ggtggccgtg tagaccacat
     2401 ggtcctcgga cacatcgaac agcgcgccgg tataggcggc ggcgcccgcc gggggcggat
     2461 cgctggtcag gcgcaacacc ggccccacgt cgagcggcac ccgttcgaac gcggcgcgct
     2521 tgtcgtggct gcgctggtag atcacgtaat cgccctcggc ccactgggcc gctcccacct
     2581 gctgcaccgc cgcgataagg ccgggcgtga ggcgcgggcg taagaagccg ggacgcgttt
     2641 tcaaaggatc cacattgacc tccgaagggc gaacagggcg gcaagccggc agcgcacagg
     2701 cgccgctgcc agtctagcaa aagcgcgccg tgcttgtcaa acccacttgc cacgatgatg
     2761 gtttcaccgc cggggcgcgg aggagtctat gg
//
LOCUS       DASUYC010000187         2846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1903550, whole genome shotgun sequence.
ACCESSION   DASUYC010000187 DASUYC010000000
VERSION     DASUYC010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1903550"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            258..500
                     /locus_tag="VF276_03005"
     CDS             258..500
                     /locus_tag="VF276_03005"
                     /inference="COORDINATES: protein motif:HMM:NF025807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX6605863.1"
                     /translation="MAEIQVKLFATLARYRPGAQHGQPFALPLVEGQDLRAAVAKLGL
                     PDEAVRHVFVNGRRVALDYVPRPGDEVAIFPPLAGG"
     gene            508..1620
                     /locus_tag="VF276_03010"
     CDS             508..1620
                     /locus_tag="VF276_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX6605864.1"
                     /translation="MTIDEAAQAFKDELLAKSPRTLKTYSAALRAFRLFLLSLKFDPA
                     TAPVSDLREDHVRQFLLSLYREPARYREPSKAEKAHPGAARRSAEPFAERTVRTYVTA
                     INRFYSFMARESYAAPNLEVVREKLRATRRRYSPSIPFVGTDTIETIINHVKSRPVQT
                     DPALEVKRRRDIALVVLLARTGLRLSEICNLQRRDIRPNDGLLLVRGGKGGKDRVVDI
                     NAEVLKVLRHYWQYADSRRAPHDTRPLADLPAIAGHDKRSPLLQPISPKTVERIVEGY
                     VKELQIGEHVTPHSFRHGFATVLVENDVNLRLVQELLGHANLSTTQVYAHISDRDRRD
                     AYQRIFGQWGNTAADTAGTDGDGGTTESAGSAENNK"
     gene            2065..>2846
                     /locus_tag="VF276_03015"
     CDS             2065..>2846
                     /locus_tag="VF276_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX6605865.1"
                     /translation="MTEKPTPERYDPASIEAKWQARWAADHTYAIDLHEESRPHYYFL
                     TMLPYPSGDLHIGHWFTMAPSDTRARYLRMRGYAVFYPIGFDAFGLPAENAAIKRNIH
                     PKQWTYANIENMRKQLRTMGAIFPWDHEVVTCDPEFYRWNQWFFIQFFKRGLAYRKFA
                     PVDWCPSCNTTLAREQVVGEDRRCERCGTPVIKKDLDQWLFKITNYAEELLDFTGLEW
                     PERIMAMQRNWIGKSEGAHVTFTTEQGHPIEVFTTRPDTLWGA"
BASE COUNT          557 a          976 c          847 g          466 t
ORIGIN      
        1 gtctcggcga ttcaggtact ggtcgcggac catgctgaat cggctggacg gcaggcccta
       61 catcgcaaaa tcagcgtggc cagccacgac actgattcgg ctgggcggca ggccggtttg
      121 aaaccggccc ctacaccgca aaatcagcgt ggtcggctac gagacggaga ttcttcgctc
      181 tgctcagaat gacaatccaa gcatgtcact ctgagcgcag cgaagagtct cttgggctac
      241 gaaaaggaca ggcgccgatg gctgaaatcc aggtcaaatt attcgccacg ctggcccgct
      301 accggcccgg cgcgcagcat gggcagccgt ttgccctacc gctggtcgag ggccaggatc
      361 tgcgcgccgc cgtggcgaag ctgggcctgc ccgacgaggc cgtgcgccac gtgttcgtga
      421 acgggaggcg ggtggcgctc gactatgtgc cgcgccccgg cgacgaggtg gcgatcttcc
      481 cgccattggc cggtggctga gatgccgatg actatcgacg aagccgccca ggcgttcaag
      541 gacgagttgc tggcgaaatc gccgcgcacg ctcaaaacct acagcgccgc gctgcgcgcc
      601 ttccgcctgt tcctgctctc gctgaagttc gacccggcca ccgccccggt cagcgacctg
      661 cgcgaagacc acgtgcgcca gttcctgctc agcctctacc gcgaacccgc ccgctaccgc
      721 gagccgagca aggccgagaa agcccacccc ggcgccgccc gccgcagcgc cgaacccttc
      781 gccgagcgca cggtgcggac ctacgtcacg gccatcaacc gcttctacag cttcatggcc
      841 cgcgagtcgt atgccgcgcc caatctggaa gtggtgcgcg aaaagctgcg cgccacccgg
      901 cggcgctact cgccgtccat ccccttcgtc ggcaccgaca cgatcgagac gattatcaac
      961 catgtcaaga gccgcccggt acagaccgac cccgcgctgg aggtcaagcg ccgccgcgac
     1021 atcgcgctgg tcgtgctgct ggcgcggacg ggcctgcgtc tgagcgagat ctgcaacttg
     1081 cagcgccgcg acatccggcc caacgacggg ttgttgctgg tgcgcggcgg caagggcggc
     1141 aaggaccgcg tggtggacat caacgccgag gtactcaagg tgctgcgcca ttactggcag
     1201 tacgccgaca gccgccgcgc gccgcacgac acccgtcccc tggccgacct gcccgccatc
     1261 gccgggcacg acaaacgctc gcccctgctg caaccgatct cgcccaagac cgttgaacgg
     1321 attgtcgagg gctacgtcaa ggagttgcag atcggcgagc atgtcacgcc gcactccttc
     1381 cgccacggct tcgccacggt actcgtggag aacgacgtga acctgcggct ggtgcaggaa
     1441 ctgctgggcc acgccaacct gagcaccacc caggtctacg cccatatcag cgaccgcgac
     1501 cggcgcgacg cctaccagcg catcttcggc cagtggggca acaccgcggc ggacaccgcc
     1561 ggcacagacg gcgatggagg cacaacagag tctgccggct cggcagaaaa taacaaatag
     1621 agcaatttca gagcgaaaat agagaatcct attgacaagc ccaccaataa ctgctattat
     1681 aacgtcagcg caggtttagc caacgaaacc ttcgcgttta gaggccactc cagccaaggg
     1741 agtggcctag ctttttgtcc gcaagctgtc agccatcagc ggtcagccgt caggagacgg
     1801 aaagcgactc tgttatccct ctgctcatcc ctcgaacagc ggtcagcggt gtgctacgca
     1861 ccgctgcgcg agcagccgtc aggcgacggt aaggtactct gttatccctc tgccaatccc
     1921 ccaagcagtt caagtttgcg cgctctattc tcggtctacc ctccgtttct gttataatgc
     1981 actatctata tctcagctaa gtcggcccaa cccgtcgtca cctgacggct gaccgctgac
     2041 ggctgacagc taagagggaa tcgcatgact gagaagccga cgccggagcg gtatgatccg
     2101 gccagcattg aggccaagtg gcaagcgcgc tgggccgccg accataccta cgccatcgac
     2161 ctgcacgagg agagccggcc ccactactac ttcctgacga tgttgcccta ccccagcggc
     2221 gatctccata tcggccactg gttcaccatg gcgccgagcg acacccgcgc tcgttacctg
     2281 cgcatgcgcg gctatgcggt gttctacccc atcggcttcg acgccttcgg cctcccggcg
     2341 gagaacgccg ccatcaagcg caacatccac cccaagcagt ggacctacgc caacatcgag
     2401 aacatgcgca agcagttgcg cacgatgggc gccatcttcc cctgggacca cgaggtcgtg
     2461 acctgcgatc cggagttcta ccgctggaac cagtggttct tcatccagtt cttcaagcgc
     2521 gggctggcct atcgcaagtt cgcgccggtc gattggtgcc cgtcgtgcaa caccacgctg
     2581 gcccgcgagc aggtggtcgg cgaggaccgg cgctgcgagc gctgcggcac gcccgtgatc
     2641 aagaaggatc tcgaccagtg gctcttcaag atcaccaact acgccgagga gctgctcgac
     2701 tttaccggcc tggagtggcc cgagcggatc atggcgatgc agcgcaactg gatcggcaag
     2761 tccgagggcg cgcatgtcac ctttacgacc gagcagggcc acccgatcga ggtcttcacg
     2821 acgcggcccg atacgctgtg gggcgc
//
LOCUS       DASUYC010000188         3850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_200530,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000188 DASUYC010000000
VERSION     DASUYC010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200530"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..1962
                     /locus_tag="VF276_03020"
     CDS             124..1962
                     /locus_tag="VF276_03020"
                     /inference="COORDINATES: protein motif:HMM:NF024810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEX6605866.1"
                     /translation="MNPQTQRTLRFPHALLLLALGLVVMMLAALAGAGGGSAAAAPLD
                     GPAGGCGPSGTPGPWATSTVGPSARYRTGATTDGTYVYVYGGSTSAATYLSDLWRWDP
                     TGQTWTQLASMPTGKSNIQGSYYDGKIYVPGGYNNSHMAEMAIYDIAGNSWSTGAPEP
                     AIRSGATAAYNGKIYVFGGNPGPSNQTLIYDIAGNSWSTGANMPVAITYGRAIRAGSY
                     MYYVGGITSGTVGTVYRYDPVGDSWATMAPLQTARTSAELMLSPDGTQIYAVNGGDAT
                     YFTGVPLAQTVEIYDIAGNSWTYGNPVVTKAAASAGGLAGGKLMIQGGVDGTSYYDQV
                     QVSVLTGGVPCTATPTATAPPTETATATATETSAPPTATATSAPPTATATSVPPTATA
                     TGVAATATATVTPCVASFSDVNPADYFYTPVGYLACHGVISGYADGTFRPSNSTTRGQ
                     MVKIVVGGFGLTAPPTTPPGGYTFADVPPAHPFFSYIETAAALGIVSGYTCGGPGEAC
                     DAFNRPYFRSGADVTRGQLSKIVVNAAGWAVQTPPVATFTDVPPAHTFYSYIETAACH
                     GVISGYADHTFRPGNNATRGQIAKIVYFALGSGASCGPAATPPAAR"
     gene            2245..2901
                     /gene="dhaL"
                     /locus_tag="VF276_03025"
     CDS             2245..2901
                     /gene="dhaL"
                     /locus_tag="VF276_03025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HEX6605867.1"
                     /translation="MSDALTFDLTRAIIYEITASMAQHADYLTQLDKAIGDGDHGRNM
                     LTGFQSVQQAIEPLPPGTPGILLRSAGMTLVTTVGGASGPLYSTAFIAAAIAAHWKEA
                     LQLADLAHMTKSAADALARRGRCRLGDKTILDALDPAARALAAAAAANQSLLYGLQAA
                     ADAARAGMEATTPMVARCGLAMQYGPGSAGHQDPGATSCYLMFDAAVRTFRRLCPQAR
                     "
     gene            3198..>3850
                     /locus_tag="VF276_03030"
     CDS             3198..>3850
                     /locus_tag="VF276_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605868.1"
                     /translation="MLEMGAADPAAGAERGLAVLCAGLETMGVPALVLDAAGAVAYVT
                     PAAARLLALDATAIRGQPWAPLWAALCPGGPVLGGAPRRMDVRCGAATVAVRAWQRPV
                     AGSPLSLVHLEDLSAEDAREREVLRIFRDSQQQADDLFALYQITQFLSAAQELDQLCA
                     IFLRELERMTGAETACLYLATPPAGLAPQVWHGLAADPPAQPDSETAAAWLQARSGD"
BASE COUNT          584 a         1382 c         1355 g          529 t
ORIGIN      
        1 aaggaggacc ccgcggcttc gattgtgcct accccgcacc gccatccccc agggggatgg
       61 ccctcgtttc atcaggacgg aactcggaat gcacacgctc gtttcatcag gaaggaactc
      121 cgtatgaacc cgcagacgca acgaaccctg cgattccctc acgcgctcct gttgctcgcc
      181 ctgggcctgg tggttatgat gctcgccgcg ctggccggcg cggggggcgg cagcgccgcg
      241 gcggccccgc ttgacggccc ggccggcggc tgcggcccca gcgggacgcc cggcccctgg
      301 gccaccagca ccgtcgggcc gagcgcccgc taccgcaccg gggccaccac cgacggcacc
      361 tacgtctacg tctacggtgg gagcaccagc gccgcgacct acctcagtga cctctggcgc
      421 tgggacccga ccggccagac ctggacccag ctcgccagta tgcccacggg caagtcgaac
      481 atccagggca gctactacga cggcaagatc tatgtgccgg ggggctataa caacagccat
      541 atggccgaaa tggcgatcta cgacatcgcc ggcaatagct ggtccaccgg ggcgcccgaa
      601 ccggcgatcc gcagcggggc caccgccgcc tacaacggca agatttacgt ctttggcggc
      661 aatcccggcc ccagcaacca gaccctgatc tacgacatcg ccggcaatag ctggtcgacg
      721 ggggccaaca tgcccgtcgc catcacctat gggcgggcca tccgcgccgg cagctacatg
      781 tactacgtcg gcgggatcac cagcgggacg gtgggcaccg tctatcgcta cgacccggtg
      841 ggcgacagtt gggcgacgat ggcccccttg cagacggcgc gcacgagcgc cgagttgatg
      901 ctctcgcccg acggcaccca gatctacgcg gtgaacggcg gggacgcgac ctacttcacc
      961 ggggtgccgc tggcccagac cgtcgagatc tacgatatcg ccggcaatag ctggacctac
     1021 ggcaaccccg tcgtcaccaa ggccgccgcc tcggcggggg gcctggccgg cgggaagctg
     1081 atgatccagg gcggcgtcga cggcaccagc tactacgatc aggtccaggt ctcggtcctg
     1141 accggcgggg tgccgtgcac cgcgaccccc acggccaccg ccccgccgac cgagaccgcg
     1201 acggccacgg cgaccgaaac cagcgccccc ccgacggcga ccgcaaccag cgcgccgccg
     1261 acggcgaccg caaccagtgt gccgccgacg gcgacggcga ccggcgtcgc cgccacggcg
     1321 acggccacag tgaccccctg cgtggcgagt ttcagcgatg tgaatccggc ggattacttc
     1381 tacacgccgg tgggctacct ggcctgccac ggggtcatca gcggctacgc cgacggcacc
     1441 ttccggccca gcaactcgac cacccgcggc cagatggtca agatcgtggt cggcggcttc
     1501 gggctgaccg cgccccccac gaccccgccc ggcggctaca ccttcgccga cgtgccgccc
     1561 gcgcacccgt tcttcagcta catcgagacg gcggcggccc tgggcatcgt cagcggctac
     1621 acctgcggcg gccccggcga ggcctgtgac gcctttaacc ggccctactt ccgctccggc
     1681 gccgatgtga cgcgcgggca gttgagcaag atcgtggtca acgcggcggg ctgggcggtg
     1741 cagaccccgc cggtggcgac gttcaccgac gtgccgcccg cgcacacgtt ctacagctac
     1801 atcgagacgg cggcgtgcca cggcgtcatc agcgggtacg ccgaccacac gttccgcccc
     1861 ggcaacaatg ccacgcgcgg ccagatcgcc aagatcgtct actttgcgct cggcagcggc
     1921 gccagttgcg gccccgccgc gactccgccc gccgcccggt aaccccgccc gcgggcaccg
     1981 aggggggact ctccggagtc ccccctcctc actgcgggag caacctcgcc ccccgcccct
     2041 ccccgccgcg gggaggggag tcacggcttc gcacatcggc ccgtcccccc tgcccgcgcc
     2101 gggaaggggg ccagggggtt aggcccgtcc ccacgcgctt gacaaagcga gtataatgtg
     2161 ggcaagcacg acccggcgca ccctcttgcg ggcgtgcggt agcggggcca tccacccata
     2221 gctatggaag agccacatct ccctatgagc gatgcactca cattcgatct gacccgcgcc
     2281 atcatttacg agattaccgc cagcatggcg cagcacgcgg actatctcac ccaattggat
     2341 aaggccatcg gcgacgggga tcatggccgg aatatgctca cgggctttca gagcgtgcag
     2401 caggccatcg agccgctgcc gcccggcacc cccggcattt tattgcggtc ggcgggcatg
     2461 acgctggtga cgacggtggg cggggcgtcg ggcccgctct acagcacggc gtttatcgcg
     2521 gcggccatcg cggcgcactg gaaggaggcg ctgcaactgg ccgacctggc ccatatgacc
     2581 aagagcgccg ccgacgcgct ggcccggcgc gggcgctgcc gcctgggcga taagacgatc
     2641 ctggacgcgc tggacccggc ggcgcgggcg ctggcggcgg cggcggcggc caatcagagc
     2701 ctgttgtacg gcctgcaagc cgccgcggac gccgcccgcg cgggcatgga ggcgacgacg
     2761 ccgatggtgg cgcgctgcgg gctggcgatg cagtatggcc ccggctcggc gggccaccag
     2821 gaccccggcg ccacctcctg ctatttgatg ttcgacgcgg cggtgcggac tttccggcgg
     2881 ctgtgtccgc aggcgcggtg acgagtggtc agtggtcagt ggtcagtggt cagtggtcag
     2941 tgggcggtga ttagccgtca gccgtcagcc gtcaggagac gggtgggggg cagtggtcag
     3001 tggtcagtgg tcagtggtca gtgggcggtg attagccgtc agccgtcagc cgtcaggaga
     3061 cgagtgacgg gtggggcttg tcattctgag cggagcgaag aatctcgtct cgttggggta
     3121 ggcgccggga ctcttcgctg cgctcagagt gacaggggcg gggggcgcgg gcggaggcgc
     3181 ggctaggaga ggcgcggatg ctggagatgg gggcggcgga cccggcggcg ggcgcggagc
     3241 gcgggctggc ggtgctctgc gcggggctgg agacgatggg ggtgcccgcg ctggtgctcg
     3301 atgccgccgg ggccgtggcc tatgtgacgc ccgcggcggc ccggttgctg gcgctggacg
     3361 cgacggcgat ccgcgggcag ccctgggcgc cgctgtgggc ggcgctgtgc cccggtgggc
     3421 ctgtgctggg gggggcgccc cggcgcatgg acgtgcgctg cggggcggcg acggtggccg
     3481 tgcgcgcctg gcagcggccc gtggccggca gcccgctctc cctggtccat ctcgaagacc
     3541 tctcagccga ggacgcccgc gaacgcgagg tcttgcgcat cttccgcgac tcgcagcagc
     3601 aggccgacga cctcttcgcc ctctaccaga tcacgcagtt cctgagcgcg gcgcaagagc
     3661 tcgaccagtt gtgcgccatc ttcctgcgcg agctcgaacg catgaccggc gcggagacgg
     3721 cgtgcctcta cctggcgacg ccgcccgccg ggctggcacc gcaggtctgg cacggcctgg
     3781 ccgccgaccc tcccgcgcaa cccgacagcg agaccgccgc cgcctggttg caggcgcgca
     3841 gcggcgacca
//
LOCUS       DASUYC010000189         2892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1945132, whole genome shotgun sequence.
ACCESSION   DASUYC010000189 DASUYC010000000
VERSION     DASUYC010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1945132"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..845)
                     /locus_tag="VF276_03035"
     CDS             complement(99..845)
                     /locus_tag="VF276_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6605869.1"
                     /translation="MTELADRLDQPISTATAPPIITMQGLVRRYKMGAGIVTAVDGID
                     LAIAAGEFVALMGPSGSGKSTLLNLLGGLDRPTAGEIVVDGVNLGRARKRDLVEYRRH
                     RVGFIFQSFHLLAHRTALENVEVPMMLSGQAPGPRRDRARALLAQVGLAARAGHHPSQ
                     LSGGEQQRVAIARALANQPRILLADEPTGNLDSATGADVMELLRDLNQGGQTLVVVTH
                     DPRVGAYADRIVHLSDGRVVKIETPEASEL"
     gene            complement(1024..2223)
                     /locus_tag="VF276_03040"
     CDS             complement(1024..2223)
                     /locus_tag="VF276_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605870.1"
                     /translation="MKRLFGLAALLIMASLVLTGCGQQITAQEIVQHMRDTAANTNSA
                     HLVVTVSGQVKGDAANATLGGTHLSDMSGQIQAELWYQKPNLLKVQLLSSNHPEYNGA
                     FALFDGTNIWAYDSSHKIAYKMDTTGLSELAGKANIPADLQGILENPNLESLIDQVLA
                     LTDYTLGPSEKVGAYQTYRLDLKPKAGSPAAQLLAGATATVWVDQATWVPVKATATAS
                     QGSGNMELSTFELNKDIPASTFQFSLPQGGHVVDLSGLAPRAVTIDQARANAKQGGYT
                     LLEPSYVPAGSTLVQVMSSRGLMGNGATTIMNYSGGSAAPNFWISELNGKDALGMGKG
                     QPDTSGPAQTVTVRNTQGHLATHADQNGGSPTAVLWWNEPGTKLTIAIGGQISQDELL
                     KIAAGLK"
     gene            2445..>2892
                     /locus_tag="VF276_03045"
     CDS             2445..>2892
                     /locus_tag="VF276_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605871.1"
                     /translation="MVKCQYPDCDGELEPGAPFCDECGRSQAPAQVAAATAGAQVRLQ
                     IQANTLAAPTLTARPQAPPRPPRPPPAPPRREPTIPVPLALGFGLVLLLLFLGCVGAL
                     AIAIVTSPLVPLPATPTPQALPGGGLAFPPVAARPAAQDGAGLTVTG"
BASE COUNT          386 a          982 c         1004 g          520 t
ORIGIN      
        1 cgtcaggatg ccgacccaga cgccaatcga ggtcagcacg gtgcgcactt tgcgccggcc
       61 caggttgctc agtccggtgc tcaaaacatc ggcaaatctc atagttcgct cgcttccggg
      121 gtctcgatct tgaccacccg cccgtcgctc aggtggacga tgcggtcggc gtaagcgccc
      181 acacgcgggt cgtgggtcac gacgacgagg gtctgcccgc cctggttcag gtcgcgcagc
      241 agttccatca catcggcgcc ggtggcgctg tccagattgc ccgtcggctc gtcggccagc
      301 aagatgcgcg gctggttcgc cagggcgcgg gcgatggcga cgcgctgctg ctcgccgccc
      361 gatagctggc tggggtggtg cccggcgcgc gccgctaggc ccacctgggc cagcagcgcc
      421 ctggcccggt cgcggcgcgg ccccggcgcc tgcccgctca gcatcatcgg cacttcgacg
      481 ttttccagcg ccgtccggtg ggccagcagg tggaaactct ggaagatgaa gccgacgcga
      541 tggcgccggt actccaccag gtcgcgctta cgcgcccggc ccaggttcac cccgtccacg
      601 acaatctcgc ccgccgtggg ccggtcgagg ccgcccaata ggttgagcag cgtcgacttg
      661 cccgaccccg acggccccat cagggccacg aactcgcccg cggcgatggc caggtcgatg
      721 ccgtccacgg cagtcacgat gcccgccccc atcttgtagc ggcggaccag cccctgcatc
      781 gtgataatcg gcggggccgt ggcggtgctg atcggttggt cgagccggtc ggcgagttcg
      841 gtcatgcgtt ccgtaccttt actggcggcc cggcagcgcg cgccgccggg cgagatcctg
      901 tcatcggcat acaacaggaa tacgccccgg cggcggcgct ggttgcatcg gccggggcgc
      961 agacacaaat cggcggccct tcgccggggg tgccggggag ggccgcctgg ttacgttgcg
     1021 ggcttacttc aggcccgcgg cgatcttgag cagttcgtcc tgcgagatct ggccgccgat
     1081 ggcgatggtc agcttcgtgc ccggttcgtt ccaccagagc accgccgtcg ggctgccgcc
     1141 gttctggtcg gcgtgggtgg cgaggtggcc ctgcgtgttg cggacggtca cggtctgcgc
     1201 cggacccgac gtgtcgggct ggcccttacc catccccagc gcgtccttgc cgttcagttc
     1261 gctgatccag aagttgggcg ccgcgctgcc gccgctgtag ttcatgatcg tcgtggcgcc
     1321 attgcccatc aggccgcggc tggacatgac ctgcaccagc gtgctgcccg ccgggacgta
     1381 gctcggctcc aacagggtgt agccgccctg cttcgcgttg gcccgcgcct ggtcgatggt
     1441 cacggcgcgc ggggccagcc cgctcaggtc gaccacatgg ccgccctgcg gcaggctgaa
     1501 ctggaaggtg ctcgccggga tgtccttgtt caactcgaat gtactcagtt ccatgttgcc
     1561 gctgccctgg ctggcggtgg cggtggcctt gaccggcacc caggtggcct ggtccaccca
     1621 gacggtggcc gtggctccgg ccagcagttg cgccgccggg ctgcccgcct tgggcttgag
     1681 gtcgagccgg taggtctggt acgcgccgac cttctcgctg gggccgagcg tgtaatcggt
     1741 cagcgccagc acctggtcga tcaggctctc cagattcggg ttctccagga tgccctgcag
     1801 atccgccggg atattggcct tgcccgccag ttcgctcaga ccggtcgtat ccatcttgta
     1861 ggcgatcttg tggctggagt cataggccca gatgttcgtg ccgtcgaaga gggcgaacgc
     1921 gccgttatac tccgggtggt tgctcgacag gagttggacc ttcagcaggt tgggcttctg
     1981 ataccagagt tcggcctgga tctgcccgct catgtccgag aggtgggtgc cgccgagcgt
     2041 ggcgttggcg gcgtcgccct tgacctggcc gctgacggtc accaccaggt gggcgctatt
     2101 cgtgttggcg gcggtgtccc gcatatgctg cacgatttcc tgcgcggtga tctgctggcc
     2161 gcacccggtc aggaccaggg atgccataat cagcagggcc gcgaggccga agaggcgttt
     2221 catcatcggg ttcctttcgc atggggcacg ctgcaggcgc gccgcgtgat cgctaattac
     2281 cgctattcta ccacggtgat atgacagggc gcccggcgcc aatgttacag catgttacag
     2341 cgaaaccggg cgcccgatct tgacattgtg catgagtgcg gtacaattta agcagcgagg
     2401 gcgcaggatc ggtgtcacgc cgacttggcg ggcggagatc gaccatggtc aagtgtcaat
     2461 acccggattg tgatggagaa ctggagccgg gcgcgccgtt ttgtgatgaa tgcgggcggt
     2521 cccaggctcc ggcgcaggta gccgccgcga cggccggggc gcaagtgcgg ctgcaaatac
     2581 aggcgaacac gctggcggcg cccaccctga cggcgcgccc gcaggcgccg ccgcgcccac
     2641 cgcgcccgcc gcccgcgccg ccgcggcgcg aaccgacgat ccccgtgccc ctggcgctgg
     2701 gctttggcct ggtgttgctg ctgctcttcc tggggtgtgt gggtgcgctg gctatcgcca
     2761 tcgtcacgtc gccgctggtc cccctgccgg ccacgcccac gccgcaggcc ctgcccggtg
     2821 gcggcctcgc tttcccgccg gtggcggccc ggcccgccgc tcaggatggc gccgggctga
     2881 ccgtgaccgg gg
//
LOCUS       DASUYC010000190         2599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_54348,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000190 DASUYC010000000
VERSION     DASUYC010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54348"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VF276_03050"
     CDS             complement(<1..482)
                     /locus_tag="VF276_03050"
                     /inference="COORDINATES: protein motif:HMM:NF039786.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6448 family protein"
                     /protein_id="HEX6605872.1"
                     /translation="MKAKAIGLAVALTALLAAGLFQPLGAAAHCDTMDGPVVKSAQEA
                     LATGNLDPVLAWVADKDVAAVRAAFEQARTVRALSPETKEMADTYFFETVVRVHRASE
                     GEPYTGLKPAGTDLGPVVPQADLALEQGAVDDLSAQVAGKVQEGIRARFNDVVSKKNY
                     A"
     gene            complement(606..1322)
                     /locus_tag="VF276_03055"
     CDS             complement(606..1322)
                     /locus_tag="VF276_03055"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX6605873.1"
                     /translation="MTATPGTTREVGQESEGVMRALAAVACFAALDQRALERMARASV
                     RRRYDAGQVVLLEGEPSRGLYVVEQGWLKMVKLNAAGREQTVRTIGPGEHFNEISVFA
                     GTPNLATVVALEPATLWVVARAAVLDLLDAIPGLARTVIEALAHEVVEMIALVEDLSL
                     RSVEGRLARLLTQQAEGGTVPRPRWATQTELAARLGTVPDVLNRAMRRLSEMGLIKVS
                     RRQIEILDPAGLEELAIWGG"
     gene            1765..>2599
                     /locus_tag="VF276_03060"
     CDS             1765..>2599
                     /locus_tag="VF276_03060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX6605874.1"
                     /translation="MPITPRVGVFVPQGWRMDLVDIPDPVEKYEAMTRVAQEAERLGF
                     DSIWLYDHFHTVPTPELETTFECWTATAGLARDTRTIRIGQMVTCNGYRNPALMAKIA
                     STVDVMSHGRLDFGIGSGWYEHEYRAYGYNYPDAPTRLKMLADALPIIKAMWTEDYAT
                     YEGKYHQVHGAINEPKGVQQPHPPLWIGGGGEKVTLKLVARYGDASNFPGLDPEAFRA
                     KAAVLRQHCETVDRDYDSIIKSTNIIAMLLDPTEVDQAEALTAPYRGNLSLEDARKSA
                     FI"
BASE COUNT          416 a          932 c          844 g          407 t
ORIGIN      
        1 gcgtagttct tcttgctcac cacgtcgttg aagcgggcgc ggatgccctc ttgcaccttg
       61 ccggcgacct gcgcgctgag gtcatcgaca gcgccctgtt cgagcgccag gtcggcctgg
      121 ggcaccaccg ggccgaggtc cgtccccgcg ggtttcaacc cggtgtacgg ctcgccctcg
      181 ctggcgcggt gcacccgcac caccgtctca aagaagtagg tgtcggccat ctctttggtc
      241 tccgggctca gcgcacgcac ggtgcgcgcc tgctcgaacg ccgcccgcac cgcggccacg
      301 tccttgtccg cgacccacgc cagcaccggg tcgagattgc ccgtcgcgag cgcctcctgc
      361 gcgctcttga ccaccgggcc gtccatcgta tcgcagtggg ccgccgcgcc caggggctgg
      421 aacagccccg ccgccagcag cgcagtcagc gcgaccgcca gtccaattgc tttcgctttc
      481 atcgtgtccc tccacaaaag tcgtatcaac gctatggtcc aggcctgtgc tcttagcata
      541 cggcctgggc gcggcggagt ctctgacttt tgtcggattt gacacggcgg tggggccggg
      601 cgcgattatc cgccccagat agccagctct tccagccccg ccgggtcgag gatctcgatt
      661 tgccggcgcg ataccttgat cagccccatc tcggacaggc ggcgcatggc gcggttgagc
      721 acgtcgggca cggtgccgag gcgggcggca agctcggtct gcgtcgccca gcgcgggcgg
      781 ggcaccgtgc caccctcggc ttgctgcgtc agcaagcggg cgagccgccc ctccacactg
      841 cgcaacgaga ggtcttcgac gagcgcgatc atctcgacca cttcgtgggc cagcgcctcg
      901 atgacggtgc gcgccaggcc ggggatggca tccaggaggt cgagcacggc ggcgcgggcc
      961 acgacccaca gggtggccgg ctccagggcc accacggtgg cgaggttggg cgtgccggcg
     1021 aagacgctga tctcgttgaa atgctcaccg gggccaatcg tgcgcaccgt ctgctcgcgc
     1081 cccgccgcat tgagcttgac catcttcagc cagccctgtt cgacgacata cagcccgcgg
     1141 gacggctcgc cttccaacaa gaccacctgc cccgcgtcat agcggcggcg gaccgaggca
     1201 cgggccatcc gctccagcgc gcgctggtcc agcgcggcga agcaggcgac ggcggccagg
     1261 gcgcgcatca cgccctcgct ctcctgccct acctcgcgag ttgtgccggg tgtcgcggtc
     1321 atatgtcaga ctccatgctg tcgcgccggg gcggtcccgg cgcgggcggg ccggcggtgc
     1381 gataccagcg ggtgcccccg tttctactat agcagcggcg ggtcaaaatt cgggtatacc
     1441 tgcgttgcaa ttcgggcaat aatccgcgga tgcgagcacc gcgcgcggca tcacgcctgc
     1501 cggcagccac ccccggcaca aaatgtgctg catggatcgt cccatatggc ccggttgcat
     1561 ggcacgcttg tacatccggg ccaccggcca acctctcccc ccgcccctcc ccgcggcggg
     1621 gaggggagtc agattgcccc cctgcccgcg ccgggaaggg ggccaggggg ttaggcccgt
     1681 cgccccctgc ccgtgccggg aagggggcag gggggttaag cccgccgcat accggataac
     1741 ctgatcaaag gagaattccg cactatgcct atcactccgc gagttggcgt gtttgtgccc
     1801 cagggctggc gcatggacct ggtcgatatc cccgacccgg tcgagaagta tgaagcgatg
     1861 acccgcgtgg cccaggaggc tgagcgcctc ggcttcgact cgatctggct ctacgaccac
     1921 ttccataccg tgcccacgcc cgaactggag acgacgttcg agtgctggac ggccaccgcc
     1981 gggctggccc gcgatacgcg gaccatccgc atcgggcaga tggtgacgtg caacggctac
     2041 cgcaacccgg cgctgatggc gaaaatcgcg tccacggtgg acgtgatgag ccacgggcgg
     2101 ctcgatttcg gcatcggctc gggctggtac gagcacgagt accgcgccta cggctacaac
     2161 tatcccgacg cccccacccg gctcaagatg ctggccgatg ccctgccgat tatcaaggcg
     2221 atgtggaccg aggactacgc cacctacgag ggcaagtacc atcaggtcca cggcgcgatc
     2281 aacgagccca agggcgtgca gcagccccac ccgcccctct ggatcggcgg cggcggcgag
     2341 aaggtcacgc tcaaactggt cgcgcgctac ggcgacgcct cgaacttccc cggcctggac
     2401 ccggaggcgt tccgcgccaa ggccgccgtc ttgcggcagc attgcgagac ggtggaccgc
     2461 gactacgact cgatcatcaa gtccaccaac atcatcgcca tgctgctgga cccgaccgaa
     2521 gtggaccagg ccgaggcgct caccgcgccc taccgcggca acctctcgct ggaggacgcg
     2581 cgcaagagcg cctttatcg
//
LOCUS       DASUYC010000191         2596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_844395,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000191 DASUYC010000000
VERSION     DASUYC010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844395"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VF276_03065"
     CDS             complement(<1..465)
                     /locus_tag="VF276_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605875.1"
                     /translation="MIGYYVLAFDGQLFETTLYPAVRRAYYQNDFSDLKRLVGDFPLL
                     QHYYQYAQTWTRRGCRRPLKHWTNGICEYNTKCPGIARLNPPDPTAEADPAWTPVIGM
                     LEERGRCMLINVPCGENRSIHHDLVDLCRYKTAGGASWELGTPAQREFIYAPP"
     gene            673..906
                     /locus_tag="VF276_03070"
     CDS             673..906
                     /locus_tag="VF276_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605876.1"
                     /translation="MPFTDPFRPQDQIDQVRKVGRLAQLLLEMRSEYERKPRRALLDQ
                     IIARIDELSALRAEMGKALEASEAAASANAPHE"
     gene            complement(1007..2356)
                     /locus_tag="VF276_03075"
     CDS             complement(1007..2356)
                     /locus_tag="VF276_03075"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX6605877.1"
                     /translation="MALEGTLEDLNIVAVLQMVASGGMTGTLRVWDATQRASIAFVQG
                     QIIHADSTLQGDRLGEVLVRTRRLTPAQLAQATALQTDQSGKRLGQILLDVGLISIED
                     LAMAVEIQIIEVMSRLLAWTRGRWQFQFTPPDPSGTIPVGAMTVDEILSGQVMLLDEP
                     EPLDEAEARLDEVYTLAAGRTRESVRGALDGESWQVLCALDGRATLSEVAQQLQIEPA
                     KVSRIAGDMLAVNLLTLAAPLGAESPPPPAGPPVPPGAPQAVPPALAIHAPGTVVVRP
                     SDTARINQVLSLLLARAEAKEVCLIDSTGSLIARQGREVHNSYPTLFALAASIFASWQ
                     ELGRSLGESKASTLLYQGAGLNICLTPVGWQAILMTLYQQTSNSGLVNFWSREASARL
                     GRVLDASTAAPAPQPAAPDLPTSPGAGAPLNGAGGSDKLSGEFRDEIARQMDDLFHS"
BASE COUNT          399 a          821 c          926 g          450 t
ORIGIN      
        1 gggcggcgcg tagataaact cacgctgggc gggcgtgcct aactcccacg acgcgccgcc
       61 cgccgtcttg tagcggcaga gatcgaccag gtcatggtgg atcgagcggt tttcgccgca
      121 ggggacattg atcagcatgc agcggccccg ctcctccagc atgccgatca ccggagtcca
      181 ggccgggtcg gcctcggcgg tggggtcggg cgggttcagg cgggcgatgc cggggcattt
      241 ggtgttgtat tcgcagatgc cgttggtcca gtgcttcaag gggcggcgac agccgcggcg
      301 ggtccacgtc tgcgcatact ggtagtaatg ttgcagcaac gggaagtcgc ccaccaggcg
      361 cttcaggtcg ctgaagtcgt tctggtagta ggcgcggcga accgcgggat acaacgttgt
      421 ctcgaacagt tgcccgtcga aggccaacac gtaatagccg atcatcgtcg tcccttgcgc
      481 tataggtgtt gcgggtgccg ggtgcgaggc gacgcctata ttacacgccc ggcgggggcc
      541 tgtcaagcgc acttgacagc cgggatcgtt tgctgtatgc tgcacgcagt tgtccaaggg
      601 gtacctcgtc cactcctttg ccgccacgga cgccactatt cattgcatac atgctagccg
      661 gaggattcgt cggtgccatt cacggaccct tttcgtcccc aggaccagat cgatcaggtg
      721 cgaaaggtgg gccggctggc ccagttgctc ctggaaatgc gcagtgaata cgagcgcaag
      781 ccgcgccggg cgcttctcga ccagattatc gcgcgcatcg acgaactgtc ggccttgcgc
      841 gccgagatgg gcaaggccct ggaggctagc gaggccgccg cttcggcgaa cgccccccac
      901 gaataaggat cgttagcctg gttgcgccgc cggtaggact tcctaccggc ggtttgacgt
      961 gccgggttgg acatagctat cgggcgggaa cgcgcgtgag gtggggttaa ctgtggaaga
     1021 ggtcgtccat ctgccgggcg atctcgtcgc ggaactcgcc gctgagcttg tcggatcccc
     1081 cggcgccgtt gaggggcgcg cccgcgccgg ggctggtcgg cagatcgggc gccgccggct
     1141 gcggcgcggg cgcggcagtc gaggcgtcga gcacacggcc caggcgggca ctcgcttcgc
     1201 ggctccagaa attgaccagc ccgctgttcg aggtttgctg gtagagcgtc atcaagatag
     1261 cctgccagcc gaccggcgtc aggcagatat tgaggccggc gccctgatag agcagggtcg
     1321 aggctttact ttcccccagt gagcggccca gttcctgcca cgaggcgaag atgctggcgg
     1381 ccagcgcgaa cagcgtcggg tagctgttgt ggacctcgcg gccctggcgg gcaatgagcg
     1441 agccggtgct gtcgatcagg cagacttcct tggcctcggc gcgcgccagc agcaagctga
     1501 gcacctggtt gatgcgcgcc gtgtcgctgg ggcggacgac cacggtgccc ggtgcgtgga
     1561 ttgcgagcgc tgggggcacg gcctgcggag cgccaggggg caccggtggg ccggcgggag
     1621 gcgggggact ctcggcgccc agcggggcgg ccagggtcag caagttcacc gccagcatat
     1681 cgccggcaat gcggctgacc ttcgcgggct cgatttgcag ttgctgggcc acctcgctca
     1741 gggtggcccg cccgtcgagc gcgcagagca cctgccagct ctcgccgtcc agggccccac
     1801 gcacgctttc gcgtgtgcgg cccgcggcca gggtatacac ctcgtccagc cgggcctcgg
     1861 cctcgtccag cggctcgggt tcgtcgagta acatgacctg gccgctgagg atctcgtcga
     1921 ccgtcatcgc gcccacgggg atggtcccgc tgggatcggg cggcgtgaac tggaattgcc
     1981 agcggccgcg cgtccaggcc agcagccgcg acatgacctc gatgatctgg atctccacgg
     2041 ccatggcgag atcctcgatc gaaatcagcc ccacgtcgag caggatctgg ccgagccgct
     2101 tccccgactg gtcggtctga agggcggtgg cctgcgccag ttgcgcgggg gtgaggcgcc
     2161 gggtgcgcac cagcacttcg cccagccggt cgccctgcag cgtggagtcg gcgtggatga
     2221 tttgcccctg cacgaacgcg atcgacgcgc gctgggtcgc gtcccacacg cgcagggtgc
     2281 cggtcatgcc gccgctggcg accatttgga gcaccgcgac gatatttagg tcttcaagcg
     2341 ttccttccaa cgccatagct ttctccggat gcaggccaat taggtggtgg ggccaagcag
     2401 gggcacgtcc gccgggttgc gccaggggcc tttgcgtggc aggcacaccc gcggacagag
     2461 gggccggcgc gtgggtctgt ccttcggctg tagtatacca tatcgccctg gagcgcggca
     2521 aaagtcccgc ctgtgatccc ccccgccgcg gctggcgggc aggcggggcg atctggtaca
     2581 atatggatca ggctgg
//
LOCUS       DASUYC010000192         2405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_515723,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000192 DASUYC010000000
VERSION     DASUYC010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515723"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VF276_03080"
     CDS             complement(<1..250)
                     /locus_tag="VF276_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605878.1"
                     /translation="MRTLHLETAAISAPNVSAADPGGFPPAAGGKGGVAGLLWHHCRS
                     VRCSFNSPTPAHFAAIRQDQPGGCVAYHTSIVILSGAKN"
     gene            253..1602
                     /locus_tag="VF276_03085"
     CDS             253..1602
                     /locus_tag="VF276_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HEX6605879.1"
                     /translation="MARFLIATMPITGHVGPGIPIARKLVERGHEVRWYTGSRFQAKV
                     EATGARYVPMHAAYDFDDLAINDAFPGRAALKDFAQLKFDLKRIFGDAGIGQVADLKA
                     IMQEYPADVILCDTGFAGARLLHEQGGPPWAAFGITALTISSRDTAPFGLGILPSTGA
                     AGHLRNRVLNWLLDNVLFRDVNANFQRIRAEMGLPPGKGGIYDSPISPFLYLQATVPE
                     FEYPRSDLPAQVAFIGPFLPDPPATFTPPAWWDELLTSKRPVIHVTQGTSATDSDQLI
                     VPALQALAGEEVLVVATTGGKPVETVKLDPLPANVRLERFIPYHYLMPHVTAMVTNGG
                     YGGVQTALAHGVPLVVAGSTEDKPEIANRVAWSGTGLNLKTKTPTSDQVRAAVRQVLA
                     EPRFRRQAQQMQAAIAQYDAPAEAVLLLEQLAATRQPVVQRAAKAMGAARLKAAGVA"
     gene            1901..>2405
                     /locus_tag="VF276_03090"
     CDS             1901..>2405
                     /locus_tag="VF276_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEX6605880.1"
                     /translation="MRRGLTTTGLLLIWTVLALYPNPMMLGRAVAQSWTPVVDADAVR
                     DLAATLPDDPHQIEAAVLGSIVPYGVPWETYGVPWYFPTPAEVLATGRGDCQGRAVVF
                     ASLLKAKGIPFTLEASFDHIWVDYPGKNPNAMENPQVSIAQKAPDQSYGFHLPADWNL
                     QQSWEIER"
BASE COUNT          414 a          831 c          768 g          392 t
ORIGIN      
        1 gattcttcgc tccgctcaga atgacaatgc tcgtgtgata ggcgacgcag ccgcccggtt
       61 gatcctggcg gatggccgca aaatgagcag gagttggact gttaaatgag cagcgcacgc
      121 tcctacaatg atgccatagc aggccggcca ccccgccctt tccgcccgca gcgggcggga
      181 agccgccggg gtccgccgcg gagacattcg gtgcagagat cgctgcagtt tccagatgga
      241 gggtacgcac gcatggcacg tttcttaatc gccaccatgc ccatcaccgg gcacgtcggg
      301 ccgggcatcc ccatcgcccg caagctggtc gagcgcgggc acgaagtccg ctggtacacc
      361 ggcagccgct tccaggcgaa ggtcgaagcc accggcgccc gctacgtccc gatgcacgcc
      421 gcctatgatt tcgacgacct ggcgatcaac gatgccttcc ctgggcgcgc cgcgctcaag
      481 gatttcgccc agctcaagtt cgacctcaag cgcatcttcg gcgatgccgg catcggtcag
      541 gtcgcggatc tcaaggcgat catgcaggag tacccggcgg atgtgatcct gtgcgatacc
      601 ggctttgcgg gcgcgcgcct gttgcacgag caaggcggcc cgccctgggc ggccttcggc
      661 atcaccgcgc tgactatctc cagccgggat acggcgccgt tcggcctggg tattttgccc
      721 agcaccggcg cggcggggca cctgcgcaat cgcgtcctca actggctgct cgacaacgtg
      781 ctgttccgcg atgtcaacgc caatttccag cgcatccgcg ccgagatggg gctgccgccg
      841 ggcaagggcg gcatctacga ctcgcccatc tcgcctttcc tgtacctgca agcgacggtg
      901 ccggagttcg agtatccgcg cagcgacctg ccggcgcagg tggccttcat cggcccgttc
      961 ctgcccgacc cgcccgccac gttcaccccg cccgcctggt gggacgaact gctcacgagc
     1021 aagcggcccg tgatccatgt cacccagggc acgtcggcca ccgactccga ccagttgatc
     1081 gtgcccgcgt tgcaggcgct ggcgggcgaa gaggtgctgg tggtggcaac caccggcggc
     1141 aagcccgtcg aaacggtgaa actcgacccg ctcccggcca atgtccggct ggaacgcttc
     1201 atcccctacc actacctgat gccccatgtg acggcgatgg tgacgaacgg cggctacggc
     1261 ggtgtgcaga cggccctggc ccacggcgtg ccgctggtgg tggccggcag caccgaagac
     1321 aagcccgaga tcgccaaccg cgtggcctgg tcgggcaccg gcctcaacct caagaccaag
     1381 acgcccacct cggaccaggt gcgcgcggcg gtgcggcagg tgctcgccga gccgcgcttc
     1441 cgccggcagg ctcagcaaat gcaagccgcc atcgcgcagt acgatgcgcc cgccgaggcg
     1501 gtcctgctcc tggaacaact ggccgccacc aggcagccgg tggtgcagcg cgccgccaag
     1561 gcgatgggcg cggcccggct caaggcggcc ggcgtcgcct gatctgccgc tgctcgcccg
     1621 tccccatgcc ggacacgccg ccggtatggg ggcggttcgc tttcaccgca gcccgattca
     1681 cgattgtgga ccgtgacatc cggtccaaca gctatattct gtgccgccca accacgaaga
     1741 acaagcctta tatcaataag cttcgcggcc ttcgttcctt cgcggttgaa ctcatggaca
     1801 gatgccggcg gaatccgttg actccggctg ctggggcggg gtataatgaa agcaccgctg
     1861 tagtgcgagc acgaagctga atagtgattg cgaggatgca atgcgtcgag ggctgacgac
     1921 aacggggttg ctgctcatct ggacggtatt ggcgctctac ccgaatccga tgatgctggg
     1981 gcgcgccgtc gcgcaaagct ggacgccggt ggtcgacgcc gacgccgtgc gcgacctggc
     2041 cgcgaccttg cccgacgacc cgcaccagat cgaagccgcc gtcctgggca gcattgtgcc
     2101 ctacggcgtg ccctgggaga cctacggcgt gccctggtac ttccccaccc cggcggaagt
     2161 gctggcgacg gggcgcggcg attgtcaggg ccgcgcggtc gtctttgcca gcctgctcaa
     2221 ggccaagggc atccccttca ccctcgaagc ctcgttcgac catatctggg ttgactaccc
     2281 cggcaagaac cccaacgcca tggaaaaccc gcaggtgagc atcgcccaga aagcgccgga
     2341 tcagtcgtat gggttccatc tgcccgccga ctggaacttg cagcaaagct gggagatcga
     2401 gcgcg
//
LOCUS       DASUYC010000193         2084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_196595,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000193 DASUYC010000000
VERSION     DASUYC010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196595"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /gene="nusG"
                     /locus_tag="VF276_03095"
     CDS             <1..416
                     /gene="nusG"
                     /locus_tag="VF276_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEX6605881.1"
                     /translation="VVPTEEEIEIRDGQRRTVQRKVFPGYVLVEMAMSDDAWYLVRNT
                     PGVTGFVGMGNKPTPLGKDEAAGILRQMEAEAPKVKVTFSPGQAVKIIDGPFADFEGR
                     VDNVNHEKGKVRVLVSIFGRETPVELDFLQVEKAV"
     gene            554..979
                     /gene="rplK"
                     /locus_tag="VF276_03100"
     CDS             554..979
                     /gene="rplK"
                     /locus_tag="VF276_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX6605882.1"
                     /translation="MAKKVRAIVKLQLPAGKATPAPPVGPALGAHQINMMGFIKEYNE
                     RTAPMAGQIIPVEITVFEDRTFTFVLKTPPAADMLRKAAGIAKGSGVPNKQKVGRVTA
                     AQIREIAETKLKDLNAYDVDAAVKMVEGTARSMGIEVVA"
     gene            1064..1786
                     /gene="rplA"
                     /locus_tag="VF276_03105"
     CDS             1064..1786
                     /gene="rplA"
                     /locus_tag="VF276_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX6605883.1"
                     /translation="MGNKHGKNYERVAALIDQTKAYTPGEAATLVKETSYVKFDPTIE
                     VHLRLGIDPRHADQVVRGTAILPHGTGKKIRILVFAAGDGERLAREAGADFVGADDLI
                     RQIEGGWVDFDVAIATPDMMGRVGKLGKILGRRGLMPNPKSGTIANPQDLPRVIADVR
                     RGKVEFRNDKTGLVHVGIGKGSFDESQIRENMLALVDAIVRAKPSGAKGTYVKSITLT
                     STMGPGIPLDVAPTLAAAQTGQ"
     gene            1979..>2084
                     /locus_tag="VF276_03110"
     CDS             1979..>2084
                     /locus_tag="VF276_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006482226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX6605884.1"
                     /translation="MMPRLEEILLRLMLAAVLGSLVGIERERLDWAAGL"
BASE COUNT          397 a          664 c          709 g          314 t
ORIGIN      
        1 tcgtcgtacc caccgaggaa gaaatcgaga tccgcgacgg ccagcgccgc acggtacagc
       61 gcaaggtctt ccctggctat gtgctggtcg aaatggcgat gtcggacgac gcctggtatc
      121 tggtgcgcaa cacgccgggc gtgaccgggt tcgtgggcat gggcaacaag cccacgccgc
      181 tgggcaaaga cgaagccgcg gggattctcc gccagatgga ggcggaggcg ccgaaggtca
      241 aagttacctt cagccccggc caggcggtca agattatcga cggccccttc gccgatttcg
      301 agggccgcgt ggacaacgtg aaccacgaga agggcaaagt ccgggtgctg gtgtcgattt
      361 ttggacgcga gacacccgtc gaactcgact tcttgcaggt cgagaaggcc gtctagggtc
      421 ttctccctgc gttggagctt gggcgaggcc acctggcccc gcccacttta ctgtatggga
      481 gggcggcatc gttgctgccc gctagcacca ggagcggtcc cgtgtggccg gctcccgaag
      541 aaaaggaaac gctgtggcta agaaagtacg cgcaatcgtc aagttgcaac tgcccgcggg
      601 caaggccacg ccggccccgc cggtcggccc cgcgctcggc gcccaccaga tcaacatgat
      661 gggcttcatt aaagagtaca acgagcgcac cgccccgatg gccgggcaga tcattccggt
      721 ggagatcacg gtcttcgagg accgtacctt caccttcgtg ctcaagactc cgcccgccgc
      781 cgatatgctg cgcaaggctg ccgggatcgc caaggggtct ggtgtgccca acaagcagaa
      841 ggtcggtcgg gtcactgcgg cgcagatccg cgagatcgcg gagaccaagc tgaaggatct
      901 caacgcctac gatgtggatg ccgccgtcaa gatggtcgaa ggcaccgcgc gcagcatggg
      961 catcgaggtc gtcgcctaac gacgctacac gcaggtggga gggtggcaat cgaaccaccc
     1021 ggcgcctcgt ccgaggcacg accacccgga ggattgcaga accatgggta acaaacacgg
     1081 taagaattac gagcgcgtgg ccgccctgat cgatcagacc aaggcgtata cgcccggtga
     1141 agcggcgacg ctggtcaagg agaccagcta tgtgaagttc gacccgacca tcgaggtcca
     1201 cctgcgcctg ggcatcgacc cccgccacgc cgaccaggtg gtgcgcggca cggcgatcct
     1261 gccgcacggc accggcaaga agatccgcat cctggtcttc gccgccggtg acggcgagcg
     1321 gctggcccgc gaggcgggcg ccgatttcgt gggcgccgac gacctgatcc gccagatcga
     1381 gggcggctgg gtcgatttcg acgttgccat cgccacgccc gacatgatgg gtcgcgtggg
     1441 taagctcggc aagatcctgg gccggcgcgg tctcatgccc aaccccaaga gcggcaccat
     1501 cgctaacccg caggacctgc cccgcgtgat cgccgacgtg cgccgcggta aggtggagtt
     1561 tcgcaacgac aagaccggcc tggtccacgt cggtatcggc aagggcagct tcgacgagag
     1621 ccagatccgc gagaacatgc tggccctggt ggacgctatt gtgcgcgcca agccgagcgg
     1681 cgccaagggc acctacgtga agagcatcac gctcaccagc acgatgggcc ccggcatccc
     1741 gctggacgtg gcgccgaccc tggccgccgc gcagaccggc cagtaagccg ccggcaattg
     1801 gcatgcaaag accccggcag ttctgccggg gttctttttt gcccgcgcgc cggcgccggg
     1861 cacgatcgat gcgggcagca cgccgggggc aggtgttaca tatctgggga cggcggtcca
     1921 tggtgaggcg cggcgccgat ctaccggccg cgatagcata ccgggtgcga agggaggaat
     1981 gatgccgaga ctcgaagaaa tcttgctgcg cctgatgctg gccgcggtcc tgggcagcct
     2041 ggtcgggatc gagcgcgagc gcctcgactg ggcggcgggg ctgc
//
LOCUS       DASUYC010000194         2666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1933550, whole genome shotgun sequence.
ACCESSION   DASUYC010000194 DASUYC010000000
VERSION     DASUYC010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1933550"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2313
                     /locus_tag="VF276_03115"
     CDS             <1..2313
                     /locus_tag="VF276_03115"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX6605885.1"
                     /translation="TQNANTVQATHDVDHKLDELKSKLPGTDIVPSFNQADYIEESLN
                     SLIREGVSGAILAILVILLFLRSVRSTLVTAVSIPVSILIAFILLNVFDISLNIMSLA
                     GLAVAVGRVVDDSIVVLENIYRHVHNGESVRSAAYTGTREVASAITASTLTTVSVFLP
                     LGFLAGLVSEIFRPFAVAVTLALLASLLVALTIVPVLATIFIKPAKAGTAPAAENTEE
                     KDTLVQRLYTPTLRLVLRNGWTKAGTLALAIIMFAGSLLPMALGGIGFSFLNFGSDKV
                     LTANIKLKPGTDIGTTSNLAAGIEAKLAENKAVNNYQTTIGTNTEGAGSGFVGLGSDS
                     AAAMTVNLNRDADVDQQANWLRDLLKATPNVVDYTVSTGGNDGGFNSSSFALVVSGPT
                     QESLQQVSTTLIDKLKQVPDLVNVTSDLAEAKPEIRIQVDPDKALQHGSTAVQIAGQV
                     RALLTQQKISQITLDSNTYDVVAGYDPATVNSIDAIRDIKVGAVNPVPLSDVATVENG
                     TGAVSITRVDQQRAVTLKGTITAKSTSGVLQEAQRIVNETPLPAGVKVQNSGVAQLQG
                     EAFGQLTTALVIAVGLVYLVMVLFFGSLSTPFVIMFSLPLAAIGSFLALWITGRPFGI
                     SAMIGILMLVGIVVTNAIVLLDMVEQHKRLGFSTYESLIYGGRIRVRPILMTAIATII
                     ALIPLALSNEGSLIAAELGTVVIGGLFTSTMLTLIVVPVVYSILDSIRHRITRRGSGT
                     PADEAPEPASVDEAREPELVGAGSQTEPAH"
     gene            2404..>2666
                     /locus_tag="VF276_03120"
     CDS             2404..>2666
                     /locus_tag="VF276_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605886.1"
                     /translation="MGERPVLDSAPPPTAAHPPGAPAPADAARPGAATAPVAISPRIC
                     EIFCALLLLILALAPNSLINTDGDVAQHLAVGHLILNGPMIPR"
BASE COUNT          478 a          910 c          845 g          433 t
ORIGIN      
        1 acgcagaacg cgaacaccgt ccaagccacc catgacgtcg accacaagct cgacgaattg
       61 aagagcaagc tgcccggcac cgatatcgtg ccctcgttca accaggccga ctacatcgaa
      121 gagtcgctga acagcctgat ccgcgaaggc gtgtcgggcg cgatcctggc gatcctggtg
      181 atcctgttgt tcctgcgcag tgtgcgctcg acgctggtca cggcggtctc catcccggtc
      241 tcgatcctga tcgcgttcat tttgctcaac gtcttcgaca tctcgctcaa catcatgagc
      301 ctggccgggt tggccgtggc cgtcgggcgc gtggtggacg atagtattgt ggtgctggag
      361 aacatctacc gccacgtcca taacggcgag tcggtgcgct cagcggcgta tacgggcacg
      421 cgcgaggtgg cctcggccat cacggcgtcc acgctgacga cggtgtcggt cttcctgccg
      481 ctgggcttcc tggccgggct ggtcagcgag atcttccgcc cgttcgccgt tgcagtcacg
      541 ctggccctgc tggcctcgct gctggttgcg ctgaccatcg tgccggtgct ggcgacgatc
      601 ttcatcaagc cggcgaaggc cggtacggcg cccgcggcgg aaaacacaga agagaaagat
      661 acgctggtgc agcgcctcta cacgcccacc ctgcgcctgg tgctgcgcaa cggctggacc
      721 aaggcgggca cgctggccct ggcgatcatc atgttcgccg ggagcctgct gccgatggcc
      781 cttggcggca tcgggttcag cttcctgaac ttcggtagcg acaaagtgct gacggccaac
      841 atcaagctga agcccggcac cgacatcggc accaccagca acctcgccgc cggcatcgaa
      901 gcgaagctgg ccgagaacaa ggccgtcaac aactaccaga cgaccatcgg caccaacacc
      961 gaaggggccg ggtcgggctt cgtcgggctg ggcagcgaca gcgccgccgc catgacggtc
     1021 aacctgaacc gcgacgccga tgtggaccag caagcgaact ggctgcgtga cctgctcaag
     1081 gccacgccca acgtcgtgga ctacactgtg agtaccggcg gcaacgacgg cggcttcaat
     1141 agctccagct tcgcgctggt ggtcagcggg ccgacgcagg aatcactgca gcaggtcagc
     1201 acgacgctca tcgacaagct gaagcaggtg cccgacctgg tgaacgtgac cagcgacctg
     1261 gccgaggcca agcccgagat ccgtatccag gtggacccgg acaaggcgtt gcagcacggc
     1321 agcaccgccg tgcagatcgc cggccaggtg cgcgccttgc tgacccagca gaagatcagc
     1381 cagatcaccc ttgacagcaa cacctatgat gtcgtggccg ggtacgatcc ggcgacggtg
     1441 aacagcatcg acgctatccg cgatatcaag gtcggcgcgg tcaacccggt gccgctaagc
     1501 gatgtggcga cggtcgaaaa cggcacgggc gcggtttcga tcacccgcgt ggaccagcag
     1561 cgcgccgtga cgctgaaagg cacgatcacg gccaagagca ccagcggcgt gttgcaagaa
     1621 gcccagcgca tcgtgaacga gacgccgttg cccgcgggcg tgaaggtgca gaacagcggc
     1681 gtggcccagc ttcagggcga ggccttcggc cagttgacca cggcgcttgt catcgccgtc
     1741 ggtctggtgt atcttgtgat ggtgttgttc ttcggctcgc tctcgacgcc gttcgtcatc
     1801 atgttctcgc tgccgctggc cgctatcggc tcgttcctgg cgctctggat taccgggcgg
     1861 cccttcggca tcagcgccat gatcggcatc ctgatgctgg tcggcatcgt ggtgaccaac
     1921 gcgatcgtgt tgctcgatat ggtggagcag cacaagcggc tcggcttcag cacctatgaa
     1981 tcgttgatct acggcgggcg catccgcgtc cgcccgatcc tgatgacggc catcgccacc
     2041 atcatcgccc tgatcccgct ggccctcagc aacgaaggct cgctgatcgc ggcggaactg
     2101 ggcacggtgg tcatcggcgg cctgttcacg tcgactatgc tcacgctgat cgtggtgccg
     2161 gtggtctaca gcatcctcga cagcatccgc caccgcatca cgcggcgcgg atcggggacg
     2221 cccgcggacg aggcgccgga accggcaagc gtcgacgaag cccgcgagcc ggaactggtc
     2281 ggcgcgggca gccagacgga accggcccac taagccaggc cgggtcctgc gtaatcgaaa
     2341 agccaggggt cgcgccgggt gcgccggcgc gaccccttca cctacctctg cacaggagtg
     2401 cccatgggcg agcggcccgt acttgactct gcacccccac ccaccgcagc gcacccaccc
     2461 ggcgccccgg ccccggcaga tgccgcgcgg cccggcgcgg caacggcccc ggtcgccatc
     2521 tcgccacgca tctgtgagat cttctgcgcc ctgctgctgc tcatcctggc gctggcgccc
     2581 aacagcctca tcaacaccga cggcgacgtg gcccagcacc tggccgtggg ccacctcatc
     2641 ctgaacgggc cgatgatccc acggga
//
LOCUS       DASUYC010000195         4763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1225368, whole genome shotgun sequence.
ACCESSION   DASUYC010000195 DASUYC010000000
VERSION     DASUYC010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4763
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225368"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VF276_03125"
     CDS             <1..640
                     /locus_tag="VF276_03125"
                     /inference="COORDINATES: protein motif:HMM:NF024920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sterol carrier protein domain-containing
                     protein"
                     /protein_id="HEX6605887.1"
                     /translation="ATHWRRRKVSRPANIYYWRDPAGAPRAYVIYTFKETAPWTRQVQ
                     IREMMALDGTALRAVFGFLRNHDSQAQEITVDQPEAGRLLALLDDPRIKVEVDPGFML
                     RILDVPAALEARRYPAGVTASLALQVTPVHGDTPPVSGQLAVNDGTGSWTPTRIAPAL
                     SLDERVLGQLYTGYLTPTQAADLGLLTVHDLAALAQAEAVFAGPRPYLPDFF"
     gene            846..2285
                     /gene="lpdA"
                     /locus_tag="VF276_03130"
     CDS             846..2285
                     /gene="lpdA"
                     /locus_tag="VF276_03130"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX6605888.1"
                     /translation="MAEPQTRAPADASAAGDSTSHFDVLVIGAGTGGYPTAIRAAQLG
                     LRTAIIEREKVGGLCLHRGCIPTKALLQSALALSTIRRAAEHGIQTGDVSFDYATMQA
                     RKDKIVGQLHRGVESLFKKYKVTQLNGTAQVAGPREVQYTPADGGAAQRLTCDHLVLA
                     TGADSRPLDGLPFDGQRILNTDQVLALTTVPASVLVVGSGAWNTEWAFLLATLGAQVT
                     LVEPGAHLLPGFDPEVGKQLERLVGRKLLKVQTGTTLSAGDFEQQGEGLRVRLGKDKT
                     VDVACALIGTGSTPRTGAGVDGLGLRMADGFVAVDEFGRTNVDGVYAIGDLIGGMMLA
                     HEAMLEGITVAETIAGRRPLPVDRTRIPRMAYTWPECASIGLTEEAAKARGGGAKTAK
                     FPYSANARAQTHAEATGFVKVVAAEDGQVLGVHIIGPNASELIGTPAVAQLMQAVAWE
                     VGAASFPHPTVSEAVWEAARMIDGAQIHL"
     gene            2317..3390
                     /locus_tag="VF276_03135"
     CDS             2317..3390
                     /locus_tag="VF276_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX6605889.1"
                     /translation="MMVTTAKRQSAAKGQPARPLHEELGLNGETLREMYYYMVLARKL
                     DERMWQLNRQGKAAFVISCQGQEAAQIGTAFALERGKDWFVPYYRDLGLMLCLGQTPR
                     MVMLSLFAKAEEPNSGGRQMPGHYGFSQANVLTTGSPVGTQWLHAAGVGLANKLLRKN
                     SAVVAGFGGEGSTSEGDWHEAMNFAGIHKLPVLFIIENNHYAISVPLPLQVGGQNLVA
                     RAAGYGMPGVGVNGLNPLECYKAVKDAADRARRGEGPTLIEMRCLRLTAHSSDDNDRT
                     YRTKEEKEADRKADPIAFFQHYLMEHGILSEAGDEEIRRKIDAEVNEATDYAEQAPAP
                     VAEDLLKHVYATEEEVAEWRSKL"
     gene            3372..4355
                     /locus_tag="VF276_03140"
     CDS             3372..4355
                     /locus_tag="VF276_03140"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017248037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX6605890.1"
                     /translation="MAVKTLIEAIRDGLREEMERDERVVVFGEDVGPRGGVFGVTDGF
                     SKQFGPDRVIDTPLAESLIAGVAIGASAGGMHPVAEVQFADFIMPAFDQIVSEAARFR
                     YRSNNDWCLPMVIRAPYGGGIHGALYHSQSVEALFGHVPGLIVVIPSNPYDAKGLLKA
                     AIRHPDPVLYFEHKGAYRLIKGDVPEGDYIVELGKCKVSREGRHLTAVAWGMMHYRCM
                     EAAEILAKDGISVEVVDVQTISPLDRETILESVKKTGKCLVVYEDNRSGGWGAEISAS
                     IAEAAFEYLDGPIMRVTGPDVPAMPYNHAQEAAFMPNTDKIVAAMRELAAY"
     gene            4384..>4763
                     /locus_tag="VF276_03145"
     CDS             4384..>4763
                     /locus_tag="VF276_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX6605891.1"
                     /translation="MANIAKVTMPQLGESVTEGTIGAWLKKPGDHIEKYESLAEVVTD
                     KVNAEIPSPYTGIIKELLVPENEPVPNGTLICTIETEAAVEEAPAGHEAPAPAAAAAP
                     AAPAAQAPAPAPAPAPAPAPAPAPA"
BASE COUNT          832 a         1666 c         1589 g          676 t
ORIGIN      
        1 cgcgacccac tggcgccgcc gcaaggtgag ccgcccggcc aacatctact actggcgcga
       61 ccccgccggg gcgccgcgcg cctatgtgat atacaccttc aaggagacgg cgccctggac
      121 ccgccaggtg cagatccgcg aaatgatggc cctggacggc acggcgctgc gggccgtgtt
      181 cggcttcctg cgcaaccacg acagccaggc gcaggaaatc accgtcgatc agccggaggc
      241 ggggcgcctg ctggccctgc tggacgatcc gcgcatcaag gtggaagtgg accccggctt
      301 catgctgcgc atcctggacg tgcccgccgc cctcgaagcc cggcgctacc cggcgggcgt
      361 cacggcgagc ctggccttgc aggtcacgcc ggtccacggc gacaccccgc cggtgagcgg
      421 ccaactggcg gtgaacgacg gcacgggatc atggacgccc actcgcatcg ccccggccct
      481 gagcctcgat gagcgcgtgc tgggccagct ctacaccggc tatctgacgc ccacccaggc
      541 ggcagacctg ggcctgctca cggtccacga cctcgccgcc ctggcccagg ccgaagccgt
      601 cttcgccggc ccccggccct acctgcccga cttcttctaa gccggccctc catagcataa
      661 ccctcgcccg tctccccctt cccgcgccgg gaagggggcc ggggggttag gcccgcccaa
      721 ctttgacaag gcgcgccgac ttgtgataca ctacagcgcg gctccgccaa ttacgccggg
      781 gtcgcgcgct ggcttcaggc cggcgcaccg cacgcgaccc agcccagggc aaggaggcca
      841 gcagcgtggc tgaaccacaa acccgcgcac cggcggatgc gtccgccgcg ggcgattcta
      901 cgtcccattt cgatgtgctc gtgatcggcg ccggcaccgg cggctatccc accgcgatcc
      961 gcgccgcgca actcggcctc cgcacggcga tcatcgagcg cgagaaagtg ggcggcctct
     1021 gcctccatcg cggctgcatc cccaccaagg cgctgctgca aagcgcgctg gccctcagca
     1081 ccatccgccg cgccgccgag cacggcattc agaccggcga cgtatcgttc gactacgcga
     1141 ccatgcaggc gcgcaaggat aagatcgtcg ggcaactgca ccgcggcgtc gagtcgctgt
     1201 tcaagaagta caaagtcacc caactcaacg gcaccgccca ggtcgccggg ccgcgcgaag
     1261 tgcagtacac gcccgcggac ggcggcgcgg cccaacggct gacgtgtgac cacctcgtgc
     1321 tcgccaccgg cgccgattcc cgcccgctcg acggcctgcc cttcgacggg cagcgcatcc
     1381 tcaacaccga ccaggtgctg gcgctgacta ccgtgcccgc ctcggtgctg gtcgtcggat
     1441 cgggcgcctg gaacaccgag tgggcgttcc tgctcgccac gctcggcgcg caggtcacgc
     1501 tcgtcgaacc cggcgcgcac ctgctgcccg gcttcgaccc cgaggtgggc aagcagttgg
     1561 agcgcctggt cgggcgcaag ctgctcaagg tgcaaaccgg cacgacgttg agcgccggcg
     1621 acttcgagca gcagggcgag ggcctgcgcg tgcgcctggg caaggacaag acggtggacg
     1681 tggcctgcgc cctgatcggc accggctcga cgccccgcac cggcgcgggc gtggacgggc
     1741 tgggcctccg catggcggac ggcttcgtcg ccgtggacga gtttggccgc accaacgtgg
     1801 acggcgtcta cgccatcggc gacctgatcg gcgggatgat gctggcccac gaggcgatgc
     1861 tcgaaggcat caccgtggcc gagaccatcg ccgggcggcg cccgctgccg gtggatcgca
     1921 ctcgcatccc gcgcatggcc tacacctggc ccgaatgcgc ctccatcggc ctgaccgagg
     1981 aggcggcgaa ggcgcggggc ggcggggcca agacggccaa gttcccctac agcgcgaacg
     2041 cgcgggcgca gacgcatgcc gaggccaccg gcttcgtcaa agtcgtggcg gccgaggacg
     2101 gccaggtgct gggcgttcat atcatcggcc cgaacgccag cgaattgatc ggcacgcccg
     2161 ccgtggccca gcttatgcag gctgtggcct gggaagtggg cgcggcctcc ttcccgcatc
     2221 ccaccgtcag cgaagcggtc tgggaagcgg cccgcatgat cgacggcgca caaattcatc
     2281 tctaaacttt ttacgtctgg ttttggacag gaggacatga tggtcacgac agccaaacgg
     2341 cagagcgcag ccaaaggcca gcctgcgcgg ccgttgcacg aagaactggg actcaacggc
     2401 gagaccctgc gcgagatgta ctattacatg gtactcgcgc gcaagctcga cgagcgtatg
     2461 tggcaactca accgccaggg caaggcggcc tttgtcatct cctgccaggg acaggaagcc
     2521 gcgcaaatcg gcacggcgtt cgccctggag cgcggcaagg actggtttgt cccctattac
     2581 cgcgacctgg ggctgatgct ctgcctgggc cagaccccgc gcatggtcat gctctccctg
     2641 ttcgccaagg ccgaggagcc gaactccggc gggcgccaga tgcccggcca ctacggcttc
     2701 tcgcaggcca acgtgctcac caccggcagc cccgtgggca cgcagtggct gcacgccgcg
     2761 ggcgtgggcc tggcgaacaa gttgctgcgc aagaacagcg ccgtcgtcgc cgggttcggt
     2821 ggcgaaggct cgacctccga aggcgactgg cacgaggcga tgaacttcgc cggcatccat
     2881 aagctgcccg tgctgtttat catcgagaac aaccattacg ccatctccgt gccgctgccg
     2941 ttgcaggtgg gcggccagaa cctggtggcg cgcgccgcgg gctacggcat gccgggcgtc
     3001 ggcgtcaacg gcctcaaccc gctcgaatgc tacaaggcgg tcaaggatgc cgccgatcgc
     3061 gcccgccgcg gcgaaggccc caccctgatc gagatgcgct gcctgcgcct caccgcgcac
     3121 tcttccgacg acaacgaccg gacgtatcgc accaaggaag agaaggaggc cgaccgcaag
     3181 gccgacccca tcgccttctt ccagcactac ctcatggagc acggcatcct gagcgaagcc
     3241 ggcgacgagg agatccgccg caaaatcgac gcggaagtga acgaggccac cgactatgcg
     3301 gagcaagccc cggcgcccgt cgccgaagat ctgttgaagc acgtctatgc cacggaggag
     3361 gaggtcgcgg aatggcggtc aaaactttaa tcgaggcgat ccgcgacggg ttgcgcgagg
     3421 agatggagcg cgacgagcgc gtggtggtgt ttggcgagga tgtgggcccg cgcggcggcg
     3481 tcttcggcgt gaccgacggc ttcagcaagc agttcggccc cgaccgggtg atcgacaccc
     3541 ccctggccga gtcgctgatc gccggggtcg ccatcggcgc gtcggcgggc ggcatgcacc
     3601 cggtggccga ggtccagttc gccgacttta tcatgccggc cttcgaccag atcgtgagcg
     3661 aggcggcgcg cttccgctat cgctcgaaca acgactggtg cctgccgatg gtcatccgcg
     3721 ccccctacgg cggcggaatt catggcgcgc tgtatcacag ccagtcggtc gaggcgttgt
     3781 tcgggcatgt gccggggctg atcgtggtca tcccgtccaa cccgtatgac gccaaggggc
     3841 tgctcaaggc cgccatccgc caccccgacc cggtgctcta tttcgagcac aagggcgcct
     3901 accgcctgat caagggcgac gtgccggagg gcgactatat cgtcgagttg ggcaagtgca
     3961 aggtgagccg cgagggccgg cacctcaccg cggtggcctg gggcatgatg cactatcgct
     4021 gcatggaagc cgccgagatc ctggccaagg acggcatctc ggtcgaggtg gtcgacgtac
     4081 agacgatcag cccgctcgac cgcgaaacga ttctcgaatc ggtgaaaaaa accgggaagt
     4141 gcctggtggt gtacgaggac aatcgcagcg gcggctgggg cgccgaaatc tcggccagca
     4201 tcgccgaggc ggcgtttgaa tacctggatg gcccgattat gcgcgtgacc gggccggatg
     4261 tgcccgccat gccgtataat catgcgcaag aagcggcgtt catgccgaat acggataaga
     4321 tcgtggccgc catgcgcgag ctggccgcct attaagatcc gcacatacag gagtaggaga
     4381 gatatggcaa acatcgcaaa agtcacgatg ccgcagttgg gcgagagcgt cactgagggc
     4441 accattgggg cctggttgaa gaagcccggc gaccacatcg agaagtacga gtcgctggcc
     4501 gaggtggtca ccgacaaggt gaacgctgag atcccgtcgc cgtacaccgg catcatcaag
     4561 gaactgctgg tgccggagaa cgagccggtg cccaacggca cgctgatctg caccatcgag
     4621 accgaggccg cggtcgaaga ggccccggcc ggccacgagg ccccggcgcc cgccgctgcc
     4681 gcggcgcccg cggcgcccgc cgcgcaagcg cccgccccgg ccccggcgcc tgcgcccgct
     4741 cccgcgcccg ctccggcccc ggc
//
LOCUS       DASUYC010000196         2823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1083570, whole genome shotgun sequence.
ACCESSION   DASUYC010000196 DASUYC010000000
VERSION     DASUYC010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083570"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1830)
                     /locus_tag="VF276_03150"
     CDS             complement(<1..1830)
                     /locus_tag="VF276_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605892.1"
                     /translation="MAGARDSRLPPAPTAEVEEAAQATLPFPRRGGAGGEGAPTRLPL
                     SREIRALGRTLGSSAPYLVTLIALVVLALAYQVRPSYSIVAGSPTDAPYWRDIHQDEE
                     DPAHTQYRWTTPDTLLYLQGVGAGAYRVAFDLTGSRPAGFPPPQVRITAGGQTLAELQ
                     PGPARASYEVQVPASATAGGDLRVTLETSNPFSPPGDKRQLGVVVYGVQIDPVGRDLA
                     LPPLDTWLPFTASAGLLALLLGLAGWGPGVVRAGGIAWALALAGFLLWDRLWITLIPG
                     VLIQVLLIAGLLLLLLGPLWRLLYRAGGIPWPEAEQRGLLAIYGLAFVARMAGQLHPQ
                     IKIIDLLFHAHRFDDVLAGRLLFTIKSNEWGGHETFYLPTPYVFMLPLQWLLNDELLT
                     IRLFTVALDTACVFLVYYLAKRAFGDGRAGLLAAGLLVTLPQAVLPFSWGITANLFGQ
                     FVGLAAIAIAVGCYERLPRPGPWLVLVGVLTAAMLSHPGNVQLTGALMGGAIVVWAWG
                     SGIGRRGAGGFALGSRKAWLALAAALVASVLIAYAGYYSHFVASQLQQLQEIRAERAA
                     KAATEGFSMITGGGVNDRTLGLIQRIARNRSTWLLDGVAGFAAE"
     gene            complement(1817..>2823)
                     /locus_tag="VF276_03155"
     CDS             complement(1817..>2823)
                     /locus_tag="VF276_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605893.1"
                     /translation="LGPELLPTFSSEPVLPLPYMLLYQFVPGIATMRVTTRVAMLITM
                     GLAVLAGFAIAWLGTRRPGWRGGRLVPAACVVLLLLESWTAPLALQPLPAGNEIPLYR
                     WLAAQPSDQVIVEYPMVYYQRGPRNVAMISLYEYYSSYHWLRMPNAALTVRPTSWTAL
                     NDELENCFPCPRSLDVLWALGVNLATVHLENLSGAQQVEFAWRSTAGQAAGLYPGEFV
                     QVADFGENKVYQIKRLGPQAPAALRSLVRPGASLVLGDPKADGGGTGAYIDALAYYLR
                     DTREYGDPDRSFGQPIAPRGAGPYDYALLYTRENPAAYGFTSADALWHNDFVVLYGRR
                     "
BASE COUNT          451 a          944 c         1069 g          359 t
ORIGIN      
        1 ttcggcggcg aagcccgcca ccccgtcgag aagccaggtg ctgcggttgc gggcgatgcg
       61 ctggatcagg ccgagggtgc ggtcgttgac gccgccgccg gtgatcatgg agaagccctc
      121 cgtggccgcc ttggccgcgc gctcagcgcg gatctcctgg agttgctgga gttgcgaggc
      181 cacgaagtgc gagtagtagc ccgcataggc gatcagcacg gaggcgacga gggcggcggc
      241 cagggccagc cacgctttgc gcgaaccgag cgcgaagccg cccgcgcccc gccgcccgat
      301 gcccgacccc catgcccaga cgacgatggc gccgcccatc agcgcgccgg taagctggac
      361 gttgccgggg tgcgagagca tggcggcggt cagcacgccc accagcacca gccaggggcc
      421 gggccggggc aggcgctcgt agcagcccac ggcgatggcg atggcggcca ggcccacgaa
      481 ttggccgaac aggttggccg tgatgcccca ggagaagggc aggacggcct gcggcagcgt
      541 gaccagcagc cccgccgcca atagcccggc gcgtccatcg ccgaaggcgc gcttcgccag
      601 gtagtagacc aggaagacgc aggcggtgtc cagggccacc gtgaacaggc ggatggtcag
      661 cagttcgtcg ttcaggagcc attgcagcgg cagcatgaag acatagggcg tgggcaggta
      721 gaaggtctcg tggccgcccc actcgttgct cttgatggtg aacagcagcc gcccggccag
      781 cacgtcgtcg aagcggtggg cgtggaacag caggtcgatg atcttgatct gcgggtgcaa
      841 ctggcccgcc atgcgggcga cgaaggccag gccgtagatc gccagcagcc cgcgctgctc
      901 ggcctccggc caggggatgc cgcccgcccg gtagagcagc cgccagagcg ggccgagcag
      961 cagcagcaac agcccggcga tcagcagcac ctggatcagc acgccgggga tgagcgtgat
     1021 ccagaggcgg tcccagagca ggaacccggc cagcgccagc gcccaggcga tgccgcccgc
     1081 gcgcaccacc cccggccccc aacccgccag ccccagcagc agcgccagca gccccgccga
     1141 ggcggtgaac ggcagccagg tgtcgagcgg cggcagggcc agatcgcggc ctaccgggtc
     1201 gatctgcacg ccgtagacga ccacgcccaa ctggcgctta tcgccgggcg gcgagaaggg
     1261 gttgctggtc tcgagggtca cgcgcaggtc gccgccggcg gtggccgagg cgggcacctg
     1321 cacctcgtag ctagcgcgcg ccgggccggg ttggagttcg gccagcgtct ggccgcccgc
     1381 ggtgatgcgc acctggggcg gggggaagcc cgccgggcgg ctgccggtca ggtcgaaggc
     1441 gacgcggtag gcgcccgcgc ccacgccttg caggtagagc agcgtatcgg gcgtcgtcca
     1501 gcggtattgc gtgtgcgcgg ggtcctcttc gtcctgatgg atgtcgcgcc agtagggcgc
     1561 gtccgtcggg ctgcccgcga cgatgctata actggggcgc acctggtagg ccagggcgag
     1621 caccacgagc gcgatcagcg tgaccaggta gggcgcgctg ctgcccagcg tgcggcccag
     1681 ggcgcggatc tcgcgcgaga ggggtaggcg ggtgggggca ccctctcccc cggcccctcc
     1741 ccgccgcggg aaggggagtg tcgcctgcgc tgcttcttcc acctccgccg tgggcgcggg
     1801 aggcaagcgg ctgtccctag cgccggccat agagcaccac gaagtcattg tgccagaggg
     1861 catcggcgga ggtgaagccg taggcggcgg ggttctcgcg ggtgtagagc agcgcgtagt
     1921 cgtaggggcc ggcgccgcgc ggggcgatgg gctggccgaa gctgcggtcg gggtcgccgt
     1981 attcgcgtgt gtcgcgcagg taataggcca gcgcgtcgat ataagcgccc gtgccgccgc
     2041 cgtcggcctt cgggtcgccc aggacgagcg aggcgccggg gcggaccaga ctgcgcaggg
     2101 ccgcgggcgc ctgcgggccg agtcgcttga tctgatagac cttgttctcg ccaaagtcgg
     2161 ccacctgcac gaactcgccg gggtagaggc ccgcggcctg acccgccgtg ctgcgccagg
     2221 cgaactccac ctgctgggcg ccgctcaggt tctccaggtg gaccgtcgcc aggttgacgc
     2281 ccagcgccca gagcacatcc aggctgcgcg ggcaggggaa gcagttttcc agttcgtcgt
     2341 tgagcgccgt ccaggatgtc gggcgcaccg tcagcgcggc gttgggcatg cgcagccagt
     2401 ggtagctgct gtaatattcg tagaggctga tcatggcgac attgcgcggc ccgcgctggt
     2461 aatagaccat cgggtattcg acaatcacct ggtccgacgg ctgggcggca agccagcggt
     2521 agagcgggat ctcgttgccg gcgggcagcg gctgcaacgc caacggcgcc gtccacgatt
     2581 cgagcaacag cagcaccacg caggccgccg ggaccagccg cccgccccgc cacccagggc
     2641 ggcgcgtgcc gagccaggcg atggcgaagc ccgccagcac ggccaggccc atggtgatca
     2701 gcatggcgac gcgcgtggtg acgcgcatgg tggcgatgcc gggcacgaac tggtagagca
     2761 gcatgtaggg caacggcagc accggctcgc tggagaacgt gggcagcaat tccgggccga
     2821 ggg
//
LOCUS       DASUYC010000197         2035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_493273,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000197 DASUYC010000000
VERSION     DASUYC010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493273"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="VF276_03160"
     CDS             complement(<1..659)
                     /locus_tag="VF276_03160"
                     /inference="COORDINATES: protein motif:HMM:NF018435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX6605894.1"
                     /translation="MPTNGTGRDLVLDLGTSTLRLARIVPGKYQGPAADGEDVIVLPA
                     LTLVDPQRHSVVAVGAQAAAIARATLPTGVEMVPPWRDGLVAAFDPAVALVRAALHEA
                     LGGERALRRLARRPRALYTLPAPATDADRQITQDVLQYAGVGTAVAVPATLAAALGAE
                     LPIRGARPRAVCDIGAGRAEIAVLAGGRIQIAGSWPLGGDWFNRAVVRAVRRRGISIT
                     PA"
     gene            complement(662..1522)
                     /gene="rfbD"
                     /locus_tag="VF276_03165"
     CDS             complement(662..1522)
                     /gene="rfbD"
                     /locus_tag="VF276_03165"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEX6605895.1"
                     /translation="MRIAITGTRGTLGATLDRQARAAGHTVLPLNRPEHDLIDRAALD
                     RALGEFGPDVVLHPAAYTDVDGCERNPDLAYTINGLGTRNVALACAQTGAALLYISTN
                     VVFDGTATAPYDEWHPRSPLGIYGKSKAAGELYVEHLLQKFYIARVSWLYGRTGNHFI
                     HKITQAADARGALSVVDDEIATPTYAEDMAAAILELVSRPHPAYGWYHLVNEGECSRY
                     AYTQRIMELTGRGSIPLTPVPLTTFQRPAPVPPYSTLANTVAAADGIRLRTWDAALAD
                     YIATTPSLQV"
BASE COUNT          279 a          688 c          773 g          295 t
ORIGIN      
        1 gccggggtga tgctgatgcc gcggcggcgc acggcgcgga cgacggcccg gttgaaccag
       61 tcgccgccga gcggccaact gcccgcgatc tggatgcgtc cgcccgccag cacggcgatc
      121 tccgcccgcc cggcgccgat gtcgcagacc gcgcggggcc gcgcgccgcg gatcggcaac
      181 tcggcgccca gggcggcggc cagcgtggcg ggcaccgcca ccgccgtgcc gaccccggca
      241 tattgcagca cgtcctgggt gatctgccgg tcggcatcgg tggccggggc gggcagggta
      301 tagagcgcgc gcgggcgccg ggccaggcgg cgcagggcgc gttcgccccc cagcgcctcg
      361 tgcagcgcgg cgcgcaccag ggccaccgcc gggtcgaacg cggccaccag gccgtcgcgc
      421 cagggcggca ccatctccac gcccgtgggc agggtggcgc gggcgatggc cgcggcctgc
      481 gcgcccaccg cgaccacgct gtgccgctgc gggtccacca gagtcagggc gggcaggacg
      541 atgacatcct cgccgtctgc cgccgggccc tggtacttgc cgggcacgat ccgcgcgagg
      601 cgcagggtgc tggtgcccag atcgagaaca agatcgcgtc cggttccatt cgtcggcatg
      661 gctagacctg caagctgggg gtagtggcga tatagtcggc cagcgcggcg tcccacgtgc
      721 gcaggcggat gccgtccgcc gccgcgaccg tattcgccag ggtggagtag gggggcaccg
      781 gcgccgggcg ctggaaggtg gtcagcggca ccggcgtgag cgggatgctg ccgcgcccgg
      841 tcagctccat gatccgctgg gtgtaggcgt agcgcgaaca ctcgccctcg ttgaccaggt
      901 ggtaccagcc gtaggcgggg tgcgggcggc tcaccagttc caggatggcg gcggccatgt
      961 cctcggcata ggtcggcgtg gcgatctcgt cgtcgaccac gctcagcgcg ccccgcgcgt
     1021 cggcggcctg ggtgatcttg tggatgaagt ggttgcccgt gcgcccgtag agccaggaga
     1081 cgcgggcgat atagaacttc tgcagcaggt gttcgacgta taactcgccc gccgccttcg
     1141 acttgccgta gatgccgagc gggctgcgcg ggtgccattc gtcgtagggc gccgtggccg
     1201 tgccgtcaaa gaccacgttg gtgctgatat agagcagcgc cgcgccggtc tgcgcgcagg
     1261 ccagggccac gttgcgcgtg cccaggccgt tgatcgtgta ggccaggtcg ggattgcgct
     1321 cgcagccgtc cacgtcggtg tacgccgctg ggtgcagcac cacgtcgggg ccgaactcgc
     1381 cgagggcgcg gtcgagggcc gcccggtcga tcaggtcgtg ctcgggccgg ttgagtggca
     1441 gcacggtatg cccggcggcg cgggcctggc ggtccagggt agcgccgagg gtgccgcgcg
     1501 tgcccgtaat cgcaatacgc ataaatctcc ttacccggca aacaacgcca atcgtgtgcg
     1561 ttcagacgtt caccgcgaag acgcgaagaa cgcgaagatt ggttttattt actcgtattc
     1621 ttcgcgtccc atacggcagg cgggcggtgt tcgtgtcggt ctcggggcag gggaagcaaa
     1681 tcccgcgccc gcccgatcct ggagcggcgc gcgcaagcgg ggcatctgcc gccatgcggc
     1741 ggcgctggct gtggtacaat ggcgcggcgc ctggtccaac tcgccggcgc cggctcgccg
     1801 ttgccgtccg tggggccggg gggagcggtc ctactgggcg ccagtgtacc atatcggtcg
     1861 ggtggcggtc aaccgcgccg cggcccggag gctcaagcct ccggctaccg tgacgaaggc
     1921 cgcctgcgcg gcctacggag tgctgagtgc tgagtcaaga cgaaagacgc gagacaggta
     1981 ggggcggacc ggcgtgtccg ccctggccgc cctaccgcac ccgccgttca gcgcc
//
LOCUS       DASUYC010000198         2761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1669914, whole genome shotgun sequence.
ACCESSION   DASUYC010000198 DASUYC010000000
VERSION     DASUYC010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1669914"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..362
                     /locus_tag="VF276_03170"
     CDS             39..362
                     /locus_tag="VF276_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HEX6605896.1"
                     /translation="MWLPLLALVIGLIIGLQFSFTVPQEYARYTAVGILAALDAILGA
                     VRAELNHAYDNKVFISGLLSNVSLAIGLTFLGDRLGVDLIIAAVVAFGVRMFNNVAII
                     RRHFL"
     gene            763..2193
                     /gene="ftsA"
                     /locus_tag="VF276_03175"
     CDS             763..2193
                     /gene="ftsA"
                     /locus_tag="VF276_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEX6605897.1"
                     /translation="MPKNQERIIVGMDVGTTKICTVVGEVGRGPEVNILGAGIAPSQG
                     LSKGMIVDIPQTVRSIALSIERAERLSGCKIGSAYVGVAGNHIASLNSRGVVAVSRGD
                     RVITNEDVTRAVDAARAIAIPQQREVLHVIPRSYVVDGHEGVHDPLGMAGFRLEVETH
                     IVTGAVTALQNLVRCVQRTGIEVDDLVLQPLASAEAVLTEPEKNLGVVLVDIGGGTTD
                     IAIFIEGSIWHTVVLPVGGNHLSNDLSIVLKVPFEGAERLKLAHGVVAPPPGVRDLYA
                     RRRPVAPPAAPLAGADRIAAEDGYGAAAQSHPVQTELLRILDDVEPEYEYDDDEVIEV
                     ETFEVGRTDRISRNLVTRILEARVEQIFGLVAAEIKRSGYDGLLPAGIVLTGGAARLP
                     GIADVAARVLHMPVRISVPQGMTGLTDTLDSPAYATSVGLALWATRHSGASGHTPQHA
                     LSPGQPRERGNEFYGRLRGFLREFLP"
     gene            2280..>2761
                     /locus_tag="VF276_03180"
     CDS             2280..>2761
                     /locus_tag="VF276_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX6605898.1"
                     /translation="MGDVENFAQIKVIGVGGGGSNAVNRMIRAEVKGVEFITVNTDAQ
                     ALLHSDAPTRIRIGDKLTKGLGSGGNPTVGAKAAEESSDELFDVLRGADMVFITAGMG
                     GGTGTGAAPVIAQIAREAGALTVGVVTRPFTFEGSRRRLSAEEGINNLKQHVDTVITI
                     P"
BASE COUNT          473 a          877 c          966 g          445 t
ORIGIN      
        1 acgcgcgagc gccggagcgg ctggcggtga cgaaatagat gtggctgccg ttgctggcgc
       61 tggtgatcgg gctgattatc ggcctgcagt tcagctttac ggtgccgcag gagtacgccc
      121 gctatacggc ggtcggcatc ctggcggcgc tggacgcgat actgggcgcg gtgcgcgccg
      181 agctgaacca cgcctacgac aacaaggtgt tcatcagcgg gctgttgtcg aacgtgagcc
      241 tggcgatcgg gctgaccttc ctgggcgacc gcctgggcgt ggatctgatc atcgccgccg
      301 tggtcgcctt cggggtgcgc atgttcaaca acgtagcgat cattcgccgg cattttctgt
      361 aggtgtccgc tgctatgcag cggcttaggg gccggtttga aaccggccca cctaccggac
      421 gaacggcacg tatcgcggtg taggggccgg tttcaaaccg gcccgcgccc ggcggatagc
      481 ggtgtagggg tcgatttgaa accggcccgc gggcgcagca caacgacggc aggaccggct
      541 tcgtgccgga ccgtaaggca ggcgaaaagt tgacataaaa acgcagccaa aactatagca
      601 ttttcgactc gggtgtggta tgataagcta tgatctacat atcgcaccct gggagatatg
      661 gctactctgt ccgtgggtgg gggccgccgg gcggttggcc ggggttcgcg aggacccttc
      721 gccgggtcgc cgtcacccga tcacagaggg gagcgtagac tgatgcctaa gaaccaggaa
      781 cggatcatcg tcggcatgga tgtcggcaca accaagatct gcacggtggt cggcgaggtg
      841 gggcgcggcc cggaggtgaa catcctcggc gcgggcatcg ccccgtcgca gggcctcagc
      901 aagggcatga tcgtggacat cccgcagacg gtgcgttcga ttgccctgtc catcgagcgg
      961 gccgagcggc tgtcgggctg caaaatcggc tcggcctatg tcggcgtggc cggcaaccac
     1021 atcgcctcgc tcaacagccg gggcgtggtg gccgtgtcgc gcggcgaccg ggtgatcacg
     1081 aatgaagatg tgacccgcgc ggtggatgcc gcacgggcca tcgccatccc ccagcagcgc
     1141 gaagtgttgc acgtcatccc gcgctcgtac gtggtggatg ggcacgaagg cgtgcatgac
     1201 ccgctgggca tggccgggtt ccggctggag gtcgagacgc acatcgtcac cggcgccgtg
     1261 accgcgctgc aaaacctggt gcgctgcgtg cagcggacgg gcatcgaggt cgacgacctg
     1321 gtgttgcagc cgctggcctc ggcggaggcg gtgctgaccg agcccgaaaa gaacctgggc
     1381 gtcgtgctgg tcgacatcgg cggcgggacg accgacatcg ccatctttat tgaaggcagc
     1441 atctggcaca ccgtggtctt gccggttggc ggcaaccacc tgagcaacga tctcagcatc
     1501 gtgctcaaag tgccgtttga gggagcggag cggctgaagc tggcccacgg cgtcgtggcg
     1561 ccgccgcccg gcgtgcgcga cctgtatgcc cggcgccgcc ctgtcgcgcc gcctgctgcg
     1621 cccctggccg gggccgaccg catcgcggcg gaagacggct atggcgccgc ggcgcaaagc
     1681 catcccgtgc agacggagtt gctgcggatt ctcgacgacg tggagccgga atatgagtat
     1741 gacgacgacg aagtgatcga ggtcgagacc ttcgaggtgg ggcggaccga ccgcatttcg
     1801 cggaacctgg tcacgcgcat cctggaggct cgcgtggaac agatcttcgg cctggtggcc
     1861 gccgaaatca agcgcagtgg gtacgatggg ctgctaccgg ccggcatcgt gctcaccggt
     1921 ggcgcggccc gcttacccgg cattgccgac gtggcggcgc gcgttctcca catgccggtg
     1981 cgcatcagcg tgccgcaagg gatgaccggg ctgaccgaca cgctcgacag ccccgcgtat
     2041 gctaccagcg tcggcctggc cctgtgggcc acgcggcact ccggcgcgag cgggcacacc
     2101 cctcaacatg cgctctcgcc gggccaaccg cgcgagcgcg gcaacgagtt ctatggtcga
     2161 ttacggggct ttttgcgcga gtttttgccg tagggcacgt tatataacag aaggagccaa
     2221 ctatgtttga tcgcaggaac cagacgcccc cgccgccgcc cggcccgtcc tatggcccca
     2281 tgggcgatgt cgagaacttc gcccagatca aagtcatcgg cgtgggcggc ggcggcagca
     2341 acgccgtgaa ccgcatgatt cgcgcggaag tcaagggcgt ggaattcatc accgtcaaca
     2401 ccgatgccca ggcgctgctg cactcggacg cgcccacgcg catccgcatc ggcgataagc
     2461 tgaccaaggg cctaggctcg ggcggcaacc cgaccgtggg cgccaaggcc gccgaggaga
     2521 gcagcgacga gctgttcgac gtgctgcgcg gcgccgacat ggtgttcatc accgcgggca
     2581 tgggcggcgg caccggcacc ggcgcggccc ccgtcatcgc gcagatcgcc cgcgaggccg
     2641 gcgcgctgac cgtgggcgtc gtcacccgcc cgttcacctt cgagggcagc cgccgccgcc
     2701 tcagcgcgga ggagggcatc aacaacctca agcagcatgt cgatacggtg atcaccatcc
     2761 c
//
LOCUS       DASUYC010000199         2902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_52585,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000199 DASUYC010000000
VERSION     DASUYC010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52585"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..582
                     /locus_tag="VF276_03185"
     CDS             40..582
                     /locus_tag="VF276_03185"
                     /inference="COORDINATES: protein motif:HMM:NF025210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX6605899.1"
                     /translation="MAHDSNVARVTVAGLPHAPLELHQVFTPLAAAGINVDAIAYALD
                     ADGHTADCSFTVAESDLARTVQVAGTVAVELGAREIRTRRDVAKVSLVGVGVEDTPGL
                     AARAFAVLAQAGIPIEMVTTSQIRITCLVPQSRMEDAVHLLHHAFNLDAGDPPPELAA
                     LRNGHVAGLEARPEVTLWNG"
     gene            570..1634
                     /gene="asd"
                     /locus_tag="VF276_03190"
     CDS             570..1634
                     /gene="asd"
                     /locus_tag="VF276_03190"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011019757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX6605900.1"
                     /translation="MERVKVGVLGASGAVGQRIVSLLIDHPWFEIGALGGSERAAGRR
                     YGDLLKPAALGSAASRPLPPALADTVVQDTHPDAFAGCRLIFSALPADVAHTVEPAFA
                     RAGFGVVSNASSFRMAEDVPLLIPEANAGHLPLIARQRESRGWRGFIVTNPNCSAIHL
                     TLALAPLHEAFGLAAVQVTTMQAISGAGLRGVAGLDIIDNVVPYIGGEEDKLEQEPQK
                     LLGRLTDSGISPAGFVVSAQCNRVATTDGHLESVAVRFKQPADIEAVRAAWREWRPLS
                     GRGLPSAPDPPVIVRDEPDRPQPRLDRDVAGGMASTVGRLRPDPILDYKFTVLGHNLV
                     RGAAGGVVLIAELLHAEEWV"
     gene            1634..2857
                     /locus_tag="VF276_03195"
     CDS             1634..2857
                     /locus_tag="VF276_03195"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEX6605901.1"
                     /translation="MELPTTGRAGITVAAGPPILDDDPSVAARNGGHPALLSHTEHWH
                     DPEPLPLDCGRTLTGLQVAYETWGRLNAAADNAVVICHALTGDAHAAGVYAGDDRPGW
                     WDPLIGPGRAIDTDRYFVVCANVLGGCRGTTGPATPAPGDGLPYGSRFPQVTVRDLVR
                     LQYRLVRALGVQSIAAVIGGSLGGMQVLEWAVLYPGLVRNAISIGASLAHSAQGIAFN
                     LIGREAIMLDPAWQDGDYYGTHRSPDRGLSIARMMGMITYQSAESMRRKFDRARVDSG
                     AENYYRPASRFQVENYLHYQGESLVRRFDANSYLVLSRAMDLHDLAQGRESMAAALAQ
                     IAPETRVLAVGISSDLLFPIDLQHAIVAAVQAAGRAATYAEIESPWGHDAFLIEYAQL
                     GAIVAGFLSGSSNAD"
BASE COUNT          416 a         1052 c         1011 g          423 t
ORIGIN      
        1 gagggagacc acatgtaacg cgacaacaag atctgcgggg tggcccacga cagcaacgtc
       61 gcccgcgtca ccgtggccgg gttgccccac gcgccgctgg aactgcacca ggtgttcacg
      121 ccgctggccg ccgcgggcat caacgtggac gccatcgcct acgccctgga cgccgacggg
      181 cacacggccg attgcagctt caccgtggcc gagagcgacc tggcgcgcac cgtgcaggtg
      241 gcgggcacgg tggcggtgga actgggcgcc cgcgagatcc gcacccggcg cgatgtcgcc
      301 aaggtgtcgc tggtcggggt cggcgtggag gatacgccgg gcctggccgc gcgggccttt
      361 gccgtgctgg cccaggcagg catccccatc gagatggtca cgacctcgca gatccgcatt
      421 acctgcctgg tgccgcagag ccgcatggaa gacgcggtcc acctgctgca ccacgccttt
      481 aacctggacg cgggcgaccc gccgccggag cttgctgctc tgcgcaacgg gcacgtcgcc
      541 ggccttgaag cacgaccgga ggtgacacta tggaacgggt aaaagtgggg gtgttgggcg
      601 cgagtggcgc cgtcgggcag cgcatcgtgt cgctgctgat cgaccacccc tggttcgaga
      661 tcggcgcgct gggcggctcg gaacgcgccg cgggccgccg ttacggcgat ctgctcaagc
      721 ccgccgcgct gggcagcgcc gccagccgcc cgctgccgcc ggccctggcc gataccgtgg
      781 tccaggacac ccatcccgac gccttcgccg gttgccgcct gatcttcagc gccctgcccg
      841 ccgacgtggc ccacacggtc gaacccgcct tcgccagagc cgggttcggc gtcgtgtcca
      901 acgccagcag cttccgcatg gccgaggacg tgccgctgct gatcccggag gccaacgccg
      961 ggcacctgcc cctgatcgcc cggcagcgcg agagccgcgg ctggcgcggc ttcatcgtga
     1021 ccaaccccaa ctgctcggcc atccacctga cgctggctct ggccccgctg cacgaagcct
     1081 tcggcctggc cgccgtgcag gtcacgacca tgcaggccat ttcgggcgcc gggctgcgcg
     1141 gcgtcgcggg gttggatatt atcgacaacg tcgtgcctta tatcggcggc gaggaagaca
     1201 agctggagca ggagccgcag aagctgctgg gccgcctgac cgattccggc atcagcccgg
     1261 cgggctttgt ggtcagcgcg cagtgcaacc gggtcgccac caccgacggc cacctggagt
     1321 ccgtcgccgt gcgcttcaag cagcccgccg acatcgaggc ggtgcgcgcg gcctggcgcg
     1381 agtggcggcc cctcagtggg cgcggcctgc ccagcgcccc tgacccgccg gtgatcgtac
     1441 gcgacgaacc tgaccgcccg cagccccggc tcgaccgcga cgtggccggc ggcatggcct
     1501 cgacggtcgg gcggctgcgc cccgacccca ttctcgatta caagttcacc gtgctggggc
     1561 acaacctggt gcgcggcgcg gcgggcggcg tggtgctcat cgccgaactg ctgcacgcgg
     1621 aggagtgggt ctgatggagt tgcctactac cgggcgggcc ggcatcacgg tcgccgccgg
     1681 cccgcccatt ctcgacgacg acccctccgt ggcggcgcgc aacggcgggc accccgcgct
     1741 gctgtcgcac accgagcact ggcacgaccc ggagccgctg ccgctcgact gcgggcgcac
     1801 gctgaccggc ctgcaggtgg cctacgaaac ctggggccgg ctcaacgccg ccgccgacaa
     1861 cgccgtggtc atctgccacg cgctgaccgg cgacgcccac gcggcgggcg tctatgccgg
     1921 cgacgaccgg cccggctggt gggacccgct gatcgggccg ggccgcgcca tcgacaccga
     1981 ccgctacttc gtcgtctgcg ccaacgtgct gggcggctgc cggggcacca ccggcccggc
     2041 caccccggcg cccggtgacg gcctgcccta cggcagccgc ttcccgcagg tcacggtgcg
     2101 cgacctcgtg cggctgcaat accggctggt gcgcgcgctc ggcgtgcaga gcatcgccgc
     2161 cgtcatcggc gggtcgctcg gcgggatgca ggtgctcgaa tgggccgtgc tctatccggg
     2221 cctggtacgc aacgccattt cgatcggcgc gtcgctggct cactcggccc agggcatcgc
     2281 ctttaacctc atcgggcgcg aggcgattat gctcgacccg gcctggcagg acggcgacta
     2341 ctacggcacg caccgcagcc cggatcgcgg cctgagcatc gcgcgcatga tgggcatgat
     2401 cacctaccag agcgccgaat cgatgcgccg caagttcgac cgggcgcgtg tagattccgg
     2461 cgcagaaaac tactaccgcc ccgcgtctcg ttttcaggtt gagaattacc tgcattatca
     2521 gggggagagc ctggtgcgcc gcttcgacgc gaacagctat ctggtcttga gccgggccat
     2581 ggacctgcac gacctggccc aggggcgcga gtccatggcc gccgcgctgg cccagatcgc
     2641 gccggagacg cgcgtgctgg ccgtcggcat cagttccgac ctgctgttcc ccatcgactt
     2701 gcagcacgcc atcgtcgccg ccgtgcaggc ggctgggcgg gcggcgacgt atgccgaaat
     2761 cgagtcgccg tggggccacg acgccttttt gatcgagtac gcgcaactcg gcgccatcgt
     2821 cgccgggttc ttgagcggga gtagcaatgc cgactgagac cgccgcgctg gggatcgtcc
     2881 agatgggcct gggccaggtg gg
//
LOCUS       DASUYC010000200         2191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1568615, whole genome shotgun sequence.
ACCESSION   DASUYC010000200 DASUYC010000000
VERSION     DASUYC010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1568615"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2191)
                     /locus_tag="VF276_03200"
     CDS             complement(<1..>2191)
                     /locus_tag="VF276_03200"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6605902.1"
                     /translation="LPPTLKSQSLLAYLILQRARPQPRERLIGMYWPEHPEERARRSL
                     TTALWHIRRCLPDDGLLSDTQQVQFNPALALWLDVDEFRARAAQNNLDSLAAAGRLYR
                     GDFLDGFYDDWIVEERNRLLDVLVAALGRLAAGYEQAGAAGAALDAALRLLAQEPLQE
                     EAHRLVMRAYCRLGRRGRALEQYERCRDELRQELDTDPAPETIALYRAILDGSFAAGP
                     VAPAGILPAAEPPARPAPGARHPLEGLTPGRMVGREADLAAIAARWRDACAGHGGLLL
                     IEGEAGVGKSRLVEEFARQVERWDLPVLRGRCFEFERSLPYQPFPDLMHTGLQLMAPR
                     ALAACPPWALAEAARLAPELAETARAGAGTEPAPAALGAATDPERLFEGLLRFFIALA
                     AGKPLLIILDDLHWAGRSCLQLIHFLTRHIATQPILLAGTFRPEALAPRHPLVALQRD
                     LQTEGLGAVQPLLPLPRAAVETMVRELSGNAEAAAPLAARLCDETEGNPFFLIELLRA
                     LVEAGVIRVDPQTWQIDLARLGHAGRPLPAGVAQAIQARTAGLTTPAREALQYAAVLG
                     QEFDFAALERMWGHGEEATLAALDQLLRRRMLLESSGSLSRDYSFTHKLIQEAVVAGL
                     SRRTRLHLHALAAAALEQVYAGQLAAMAGELGFHYRQAQEHDPAVAGKAVAYLILAGD
                     RARDVDMLREAIDYYEQALGLLRAQPPGEQAARVCMKLGLVRHWLRD"
BASE COUNT          310 a          768 c          828 g          285 t
ORIGIN      
        1 aagtcgcgca gccagtggcg gaccaggccg agcttcatgc acacgcgcgc cgcctgttcg
       61 ccggggggct gggcgcgcaa gagccctagc gcctgctcgt aataatcgat cgcttcccgc
      121 agcatatcca cgtcgcgcgc cctgtcgccc gccaggatca ggtaggcgac ggctttgccg
      181 gccacggccg ggtcgtgctc ctgggcctgg cggtagtgga agccgagttc cccggccatg
      241 gcggcaagct gccccgcata gacctgttca agggccgcgg cggccagggc gtgcaggtgc
      301 agccgggtcc ggcgagacag gccggccacc acggcttcct ggatcagctt gtgcgtgaag
      361 ctgtagtccc ggctcaaact gccgctgctc tccagcagca tccggcgccg cagcaactga
      421 tccagcgcgg ccagcgtggc ctcttcgccg tgcccccaca tccgctccag ggcggcaaaa
      481 tcgaactcct gcccgagcac ggcggcgtac tgcaacgcct cgcgggcggg cgtggtcaag
      541 ccggcggtgc gggcctggat cgcctgcgcg acccccgcgg gcagcgggcg gcccgcgtgg
      601 ccgaggcgtg cgagatcgat ctgccaggtc tgcgggtcca cccggatcac gcccgcctcg
      661 accagggcgc gcagcagttc gatcaggaag aacgggttgc cctcggtctc gtcgcacagc
      721 cgcgcggcca gcggcgccgc cgcctcggcg ttgcccgaca gttcgcgcac catggtctcg
      781 acggcggccc gcggcagggg gagcagcggt tgcacggcgc ccaggccctc ggtctggaga
      841 tcgcgctgca aggcgacgag gggatgccgg ggtgccaggg cttcggggcg gaaggtgccg
      901 gcgagcagga tcggctgcgt ggcgatgtgc cgcgtgagga agtggatcag ttgcaggcag
      961 gaccggccgg cccaatgcag gtcgtccagg ataatcaaca gcggtttgcc cgccgccaga
     1021 gcgatgaaga agcgcagcag cccctcgaac aggcgctccg ggtccgtggc ggcgcccagg
     1081 gcggccgggg ccggctccgt gccggccccg gcgcgggccg tttcggcgag ttccggcgcc
     1141 agccgggcgg cctcggccag cgcccagggc gggcacgccg ccagagcacg cggagccatg
     1201 agctgcaggc cggtgtgcat caggtcgggg aacggctgat agggcaggga tcgctcgaac
     1261 tcgaagcagc gcccgcgcag cacgggcagg tcccagcgct ccacctgccg ggcgaactcc
     1321 tcgaccagcc gggacttgcc caccccggcc tcgccctcga tcagcagcaa gccgccgtgc
     1381 ccggcgcagg cgtcgcgcca gcgcgcggcg atggcggcca ggtctgcctc gcgtccgacc
     1441 atccgtcccg gcgtcagtcc ttccagcgga tggcgcgcgc ccggtgccgg acgcgcgggc
     1501 ggctccgcgg caggcaggat gcccgcgggc gcgaccggcc cggcggcgaa gctgccgtcc
     1561 aggattgcgc ggtagagcgc gatggtttcc ggcgcggggt cggtatccag ctcctggcgc
     1621 aattcatccc ggcagcgctc gtactgttcg agggcgcggc cgcgccggcc caggcggcag
     1681 taggcgcgca tgaccaggcg gtgtgcttct tcctgcaagg gctcctgggc cagcagccgc
     1741 agggcggcgt ccagggccgc gccggcggca ccggcctgct cgtagcccgc cgccagccgc
     1801 ccgagggccg ccaccaacac gtccagcagg cggttacgct cttcgacgat ccagtcgtcg
     1861 tagaagccgt cgaggaagtc gccgcgatag agccggccgg cggcggccag gctgtccagg
     1921 ttgttctgcg cagcgcgcgc ccggaactcg tctacgtcga gccagagcgc gagcgcgggg
     1981 ttgaactgga cctgctgggt atcgctgagc agcccatcgt cgggcaggca gcggcggatg
     2041 tgccagaggg cggtggtgag cgagcggcgg gcgcgttcct ccgggtgctc cggccagtac
     2101 atgccgatca ggcgctcgcg aggctgcggg cgggcgcgct ggaggatcaa ataggccagg
     2161 agcgactgcg atttgagggt gggcggcaac a
//
LOCUS       DASUYC010000201         3865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_52483,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000201 DASUYC010000000
VERSION     DASUYC010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52483"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1223
                     /locus_tag="VF276_03205"
     CDS             <1..1223
                     /locus_tag="VF276_03205"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX6605903.1"
                     /translation="HLAGLASADLGLPVRAVALTVPANAEDRVRVSVQAALAAAGLDL
                     VRIVNAPTAALLALRRLLPGDTRLRDGLVALVDIGGGTTDVSIARLDGAGVTILATAG
                     DPYLGGVELRARLAAGANAGFRAAGVDLLAPAGAATGHSRAGELALLRAADEAMTALS
                     SAAQADLLLDHGAGFGRDLWARVARTEFEAWIAPDLVRLATLCGQALRRAGLPASALD
                     AVALAGGCAHIPAIQRAIAAAFGRVPADLIIREPAALAAYGAALQAGIALGQVAERVQ
                     DVTPYPLGIAAFNAPYPGGVELFSEVVARGTPIPTPGPTARAACRRTFYTRVPGQDAM
                     TLQVLQYRGADEPVAVPPAACETLGEWTLTGIAPHGQVPVDVTFHIDADGILHLTAQE
                     QGTRNILRQAIARW"
     gene            1410..2189
                     /locus_tag="VF276_03210"
     CDS             1410..2189
                     /locus_tag="VF276_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605904.1"
                     /translation="MDPNEQTRPRMTVVVAQCVQRSFISPLAGPDPRLKPCHLHCGYV
                     NSIRLLGGGAQHAPNHLETFLRRAHGLDGADRGSDRVHVVYVDDEHYDDGNELTAAHF
                     AQFGRHCVPGEDGYLGTGETAEWRALRNGRTHVVKTPGLNDNMYGTGFIPVLTAICQE
                     AEEVYGIAPGDIRILNIGGWSIIKVLFCGVWELMHHGIPDWRDPTNREATIRFNDVAI
                     CDALTYSVQREQHTVALEVVEMIGGQVLRSVDDALAYCGLS"
     gene            2288..2851
                     /locus_tag="VF276_03215"
     CDS             2288..2851
                     /locus_tag="VF276_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605905.1"
                     /translation="MGYFRANDRYLQGPPEAGVDPPWDVQAQEAGRPGSVRLAWRRPY
                     TAVDAFTVYGRRADDGPAAEWTSLCEVVGTATEAVLHLAPGGWQLAVGARRGQTTSPL
                     SPPTLWLPPQAAVVEVAPPAPLPPVPPPAPPAPPARVPPPAPPTPPPTPPPAEPAPEF
                     DWQAIDHALRDNSAHLGRHGVFIRPPR"
     gene            3074..3409
                     /locus_tag="VF276_03220"
     CDS             3074..3409
                     /locus_tag="VF276_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605906.1"
                     /translation="MDTQNTRTTATPGLAYCTCCAPAQILIPRDDLGEPGRWAVCLAT
                     GAWYEARDGRFVATAAPREGTIVPAPARTAAPTPVGWRASDGGWTGGDARPAIVPGVR
                     IDLSKESYA"
     gene            3423..>3865
                     /locus_tag="VF276_03225"
     CDS             3423..>3865
                     /locus_tag="VF276_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605907.1"
                     /translation="MEQAIVLPGTGDEAMEVRWTVDLAAAGRTAQWAHGNPALLAQEV
                     AEMGTYFPRWVLSVSDGAVLTRCARCADLLVFREGKLRCLRCGRAGQGKHLAWTGHLP
                     VPVAGLPRALERIHAHPHPGYPLVRLGATLLWLVPLLAGYPADWPR"
BASE COUNT          583 a         1409 c         1378 g          495 t
ORIGIN      
        1 gccacctggc cggactggca agcgccgacc tggggcttcc agtgcgcgcc gtggcgctga
       61 cggtgcccgc caacgccgag gaccgcgtgc gggtgagcgt gcaggcggcg ctggcggcgg
      121 cggggctgga cctagtgcgc atcgtcaacg cgcccacggc ggcgctgctg gccctgcgcc
      181 ggttgctgcc cggcgacacc cggctgcgcg acggcctggt ggccctggtc gatatcggtg
      241 ggggcaccac ggatgtctcc atcgcccggt tggacggcgc gggcgtgacg atcctggcga
      301 cggcgggcga tccctacctg ggcggggtcg agctacgggc gcggctcgcg gcgggcgcca
      361 atgccggctt ccgggcggcg ggcgtcgatc tgctcgcccc ggcgggcgcc gccaccggcc
      421 acagccgggc gggcgaactg gccctgctgc gcgcggcgga cgaagccatg accgcgctga
      481 gcagcgccgc gcaggccgat ctgctgctgg accacggcgc gggctttggc cgcgatctct
      541 gggcgcgagt cgctcgcacc gagttcgagg cgtggatcgc gccggacctc gtgcggctgg
      601 cgaccctctg cggccaggcc ctacgccggg ccgggctgcc ggcgagcgcc ctggacgccg
      661 tggccctggc gggcggctgt gcccacatcc cggcgatcca gcgggccatt gccgccgcct
      721 tcggtcgcgt cccggcagac ctgatcatcc gcgaaccggc ggcgctggcc gcctacgggg
      781 ccgccttgca ggccgggatc gccctcggcc aggtcgcgga gcgcgtgcag gatgtcaccc
      841 cgtatccgct gggcatcgcc gccttcaacg cgccctatcc gggcggggtg gagttgttca
      901 gcgaggtggt ggcgcgcggc acgccgatcc cgacgcccgg tcccaccgcc cgcgcggcct
      961 gccggcgcac cttctacacc cgcgtgcccg gccaggacgc gatgacgctg caggtgttgc
     1021 agtaccgtgg cgcggacgaa ccggtcgccg tgccgcctgc cgcctgcgaa acgctgggcg
     1081 agtggaccct gaccggcatc gcgccgcacg gccaggtccc ggtggacgtg accttccaca
     1141 tcgacgccga cggcatcctg cacctgacgg cgcaggaaca gggcacgcgc aacatcttgc
     1201 gccaggcgat tgcgcgctgg taacgagccg tcagccgtca gccatcagcc gtcaggagac
     1261 ggggagacgg cagacgggca gacggaaagt agagattatc ggccaacaaa tcaacacgag
     1321 gccgcagacc caattactga ttgctgacgg ctgacggctg acagctgacg gctgacggct
     1381 gacggctgac agctaaaagg aggcacaaca tggacccgaa cgaacagacc cgcccacgta
     1441 tgaccgtggt ggtcgcgcag tgcgtgcagc gcagctttat cagcccgctg gccgggccgg
     1501 acccgcggct gaagccctgc cacctgcatt gcggctatgt gaacagcatt cgcttgctgg
     1561 gcggcggcgc gcagcacgcg cccaaccacc tggagacctt cctgcgccgg gctcacggcc
     1621 tggacggcgc ggaccggggc agcgaccgcg tccacgtggt ctacgtggac gatgagcact
     1681 acgacgacgg caacgaactg accgccgccc acttcgccca gtttggccgc cattgcgtgc
     1741 cgggcgagga cggctacctg ggcaccggcg agaccgccga gtggcgcgcc ctgcgcaacg
     1801 ggcgcaccca cgtcgtgaag acgccgggcc ttaacgacaa catgtacggc accggcttca
     1861 tccccgtgct gacggccatc tgccaggaag cggaggaagt ctacggcatc gcgcccggcg
     1921 acatccgcat cctcaacatc ggcggctgga gcatcatcaa ggtgctgttc tgcggtgtct
     1981 gggagctgat gcaccacggc atccccgact ggcgcgaccc gaccaaccgc gaggcgacca
     2041 tccgcttcaa cgacgtcgcc atctgcgacg cgctgaccta cagcgtgcag cgcgagcagc
     2101 acaccgtggc cctggaagtc gtcgagatga tcggcggcca ggtgctgcgc agcgtggacg
     2161 acgcactggc ctattgcggg ctgagctaac gcgggccggt ttcaaaccgg cccctacaga
     2221 ggacggctgg ggtaggagcc ggtttcaaac cggcccctac agaggacaag gatcagaggg
     2281 atcggcgatg gggtactttc gcgccaacga ccgctatctg caaggcccgc ccgaggccgg
     2341 ggtcgatccg ccgtgggacg tgcaggcgca ggaagcgggc cggcccggca gcgtccgcct
     2401 agcgtggcgg cggccctaca cggcggtgga cgccttcacc gtgtatggcc gccgggcgga
     2461 cgacggcccg gcagcggagt ggacgtccct atgcgaggtg gtgggcacgg ccacggaggc
     2521 cgtgctgcac ctggcgccgg gcggctggca gttggcggtg ggcgcgcggc gggggcagac
     2581 aaccagcccg ctctcgccgc ccacgctctg gctgccgccg caagccgccg tggtcgaggt
     2641 cgcgccgccc gcgccgctgc ccccggtccc gccacccgcg ccacccgcgc cgccggctcg
     2701 ggtgccgccg cccgcgccac cgaccccgcc gccaacgccg cccccggcgg aaccggcccc
     2761 ggagttcgac tggcaagcca tcgaccacgc cctgcgcgac aactcggccc acctgggccg
     2821 ccacggcgtc ttcattcgcc cgccccggta acgggcggcg gggcggcacc gaggctctgg
     2881 gatgtgaggc gaggaacacg cttacgagca acgagtacgg gcgcgataaa tcgcgccgct
     2941 acgaataaga gcagcttgaa tcacaatacc gcataaccca tcaccaatta ctgatcactg
     3001 accactgacc actgaccact cgtacctgac ggctgatagc tgacggctga tagctcggaa
     3061 aggagcaaca accatggaca cgcaaaatac ccgcaccacc gcgacccccg gcctggccta
     3121 ctgcacctgc tgcgcgccgg cgcagatcct gatcccgcgc gatgacctgg gcgagccggg
     3181 gcgctgggcc gtctgcctgg cgaccggcgc ctggtatgag gcgcgcgacg ggcgcttcgt
     3241 cgccaccgcc gccccgcgcg agggcactat cgtgcccgcc cccgcgcgta ccgcagcgcc
     3301 cacgcccgta ggctggcggg ccagcgacgg cggctggacc ggcggcgatg cccgcccggc
     3361 catcgtgccc ggcgtgcgca tcgacctgag caaggaaagc tacgcgtagg agggccgcgg
     3421 ccatggaaca ggcaatcgtg ctgccgggca ccggcgacga ggcgatggaa gtgcgctgga
     3481 cggtggacct ggccgccgcc gggcggacgg cgcaatgggc gcacggcaat cccgccctgc
     3541 tggcccagga agtggccgag atgggcacct actttccgcg ctgggtactg agcgtcagcg
     3601 acggcgccgt gctcacccgc tgcgcgcggt gcgccgatct cctcgtcttc cgcgaaggca
     3661 agctgcgctg cctgcgctgc gggcgggccg ggcagggcaa gcacctggcc tggaccggcc
     3721 acctgccggt gccggtggcc gggctgccgc gcgcgctgga acgcatccac gcccacccgc
     3781 accccggcta tccgctggtg cgcctgggcg ccacgctgct ctggctcgtg ccgctgctgg
     3841 ccggctaccc ggcggactgg ccgcg
//
LOCUS       DASUYC010000202         4149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1124588, whole genome shotgun sequence.
ACCESSION   DASUYC010000202 DASUYC010000000
VERSION     DASUYC010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124588"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="VF276_03230"
     CDS             complement(<1..1023)
                     /locus_tag="VF276_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HEX6605908.1"
                     /translation="MTRTHPYLPGLDEPAPKTFRDGTHRLIAPAETVARVRRFMPVMG
                     ITRIANITGLDCIGIPVVMVCRPNARSLSVAQGKGLDLPAAQASGLMEAIESYHAEHI
                     TLPLKLATYEELRYTHQVVDPAQLPRTAGGVFHPHLALPWIAGYDLLQDEPVWVPYEM
                     VHLNYTVAMRRDTPTFVASSNGLASGNHLLEAISHAICEVVERDATTLWSLRDPATQA
                     ATRIDPATVDDPACRVVLDKYTQAGIAVGIWEITSDLGIPAFLCRIVDATEEPLRRLC
                     NGDGMGCHPAREVALLRALTEAAQSRLTWIAGSRDDRYHADYVHLRDPVVLQQRRAEL
                     TALAPGR"
     gene            complement(989..2164)
                     /locus_tag="VF276_03235"
     CDS             complement(989..2164)
                     /locus_tag="VF276_03235"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6605909.1"
                     /translation="MANRHATCDVAVIGGGPAGATAALTLAQTGYQVLLVEQQSRPAF
                     KVGEALPPAARPLLRDLGLLARLEAGGHLPSYGIQAAWGGPALQSTDFIRDPHGPGWH
                     LDRAAFDAGLRAAAREAGAGVREGARAIAAARTGGGWHVTLAAPAGPPDIAARWLIDC
                     SGRARWLARQLRVARHSDDRLLAFVTRFRPAPPGFARDHDARTLVEAGADGWWYTALL
                     PDGERVVVYHTDARNATSAPARTATGYGDLLAVTTHMRALLTTYNYQLGHGPRPTAAN
                     STRLAGFAGAGWIAAGDAAIAFDPLSSQGIFTALYAGLHAARALHAHLTGDAGALAAY
                     DRNLTAIYAAYLHHRAVFYRLESRWAQQPFWHRRRQDPLERGIAPSHDAHPPIPARS"
     gene            complement(2175..4061)
                     /locus_tag="VF276_03240"
     CDS             complement(2175..4061)
                     /locus_tag="VF276_03240"
                     /inference="COORDINATES: protein motif:HMM:NF036537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LodA/GoxA family CTQ-dependent oxidase"
                     /protein_id="HEX6605910.1"
                     /translation="MDAARQIVRAVIHPGIGIARVGNSAGDYFIGPEVPYAMPPPAGG
                     YKDATGALKRQAARFRLFGYDRNGAVVQELTADDADITWTVHVANKKAAWYNFEKALD
                     IPEAIPCPRRNAAYPLDQRGDLIIDPGPRSVTGRDQDGAAAQFDTGTFCGHPVYLGEL
                     RTDAQGRLLFLGGRGQSGTPFTHNPLTTYGNNNGWHDDISDGPVTAQVTLNGRSIPVD
                     PAWVVVAPPNYAPDLISIQTMYSVMVDAYQFWWLAPITRPSFTQHIYPILRRLCDLAW
                     VNYGFHIQFGWGAPYDFLRPHYLAQLASKHAAYREVRRQIFNLFRDPAATTLDVKGWP
                     PMYGDDFVMPSGGPRTFLALTTTEYGYLQQWAEGNFIADWEPDTAAAAPEIDDVPLAQ
                     QPDTLDRAALEFCLGGPFHPGCEMTWPMRHVTMYYAPFRIRPRQPGQPEPDYGDQLTP
                     DSINAENGPLYANGPGDITRWMAVPWQADSASCRAGYEKDYDPYLPTFWPPRVPNHVL
                     RAEEYDRARNPNLSIEERMRAFHTRATWLRGLKGHYLDWINEMVTEFGKLGIVERREG
                     PADDLFPPVMYVESRPGVEPAAPVDRNLVAGPVEKVTRHRPHARRTGPVAPSDAGIGS
                     RGQR"
BASE COUNT          618 a         1324 c         1556 g          651 t
ORIGIN      
        1 ccggcccggt gcgagcgcgg tgagttcggc ccggcgctgc tgcagcacga ccgggtcgcg
       61 caggtgcaca taatccgcgt ggtagcgatc atcgcgcgat ccggcgatcc aggtcaagcg
      121 gctctgggcg gcctcggtga gcgcgcgcag cagcgcgact tcgcgcgcgg ggtggcagcc
      181 catcccgtcg ccgttgcaca gccggcgtaa gggctcctct gtcgcgtcca cgatccggca
      241 gagaaacgcg gggatgccaa ggtccgaggt gatctcccag atcccgacgg cgatcccggc
      301 ctgggtatac ttgtcgagga cgacccggca cgccgggtcg tcgactgtag ccgggtcgat
      361 ccgggtagca gcctgcgtgg cggggtcacg gagagaccac aacgtggtgg cgtcgcgctc
      421 gaccacttcg caaatggcat ggctgatcgc ctccagcagg tggttgccgg atgccagccc
      481 attggaagat gcgacaaaag tgggcgtgtc gcggcgcatc gcgacggtgt agttcaggtg
      541 caccatttcg tagggcaccc agaccggctc gtcctgcagc agatcgtagc cggcgatcca
      601 gggcaaggcc aggtgcgggt gaaacacccc gcccgccgtg cgcggcaatt gcgccgggtc
      661 gactacctgg tgggtgtaac gaagctcctc ataggtggcg agtttcaggg gcagggtgat
      721 gtgctcggcg tggtagcttt caatcgcttc catcaggccc gatgcctggg ccgcgggcag
      781 atccagaccc ttaccctggg ccacactcag cgagcgcgca ttggggcggc agaccatcac
      841 tacggggatg ccgatgcaat ccaggccggt gatgttggcg atgcgggtga tgcccatgac
      901 cggcatgaac cgccgcaccc gtgccacggt ttcggcgggc gcaattaggc ggtgcgtgcc
      961 gtcgcggaag gttttggggg ccggctcgtc aagaccgggc aggtatgggt gggtgcgcgt
     1021 catgggatgg agcaatcccc ctctccagcg ggtcctgccg gcggcggtgc cagaacggct
     1081 gctgcgccca gcgcgactcc agccggtaaa agaccgcgcg gtggtgtaaa taggcggcgt
     1141 atatggcggt gaggttgcga tcataggccg ccagtgcgcc ggcatctccg gtcaggtgcg
     1201 cgtgcagggc gcgcgccgcg tgcagcccgg catagagcgc ggtgaagatg ccttgcgaag
     1261 aaaggggatc gaaggcgatg gcggcatcgc cggcggcgat ccaccccgcg cccgcgaacc
     1321 cggccagtcg cgtgctgttc gccgcggtgg ggcgggggcc gtggccgagt tgatagttat
     1381 acgtcgtgag caaggcgcgc atatgggtgg tcacggccag caggtcgccg tagcccgtgg
     1441 ccgtgcgcgc gggggcgctt gtcgcgttgc gggcatcggt atgatacacg accacgcgct
     1501 cgccgtccgg cagcagggcc gtgtaccacc agccgtccgc cccggcttcg accagcgtgc
     1561 gcgcgtcgtg atcgcgggcg aaaccgggcg gggccgggcg gaagcgcgtc acgaaggcca
     1621 ggagccggtc gtcgctgtgc cgggccacgc gcaattgccg ggccagccag cgcgcgcggc
     1681 cactgcaatc gatcagccag cgggcggcga tgtccggcgg gcccgccggg gcggccaggg
     1741 taacgtgcca tccgccgccg gtgcgcgcgg cggcgatcgc gcgggcccct tcgcgtaccc
     1801 cggcgcccgc ctctcgggcg gccgcgcgca acccggcgtc aaaggcggca cggtcgagat
     1861 gccagccggg cccgtgcgga tcgcggataa agtcggtgct ctgcagggcg gggccgcccc
     1921 aggccgcctg gataccgtag gacggcagat ggccgcccgc ctccagccgg gcgagcaggc
     1981 ccaggtcccg gagcagcggg cgggccgccg ggggcagcgc ctcgcccact ttaaaggcgg
     2041 ggcggctctg ttgctcgacc agcagcacct ggtacccggt ctgggccagg gtgagcgccg
     2101 cggtcgcccc cgccgggccg ccgccgatca ccgccacatc gcaggtagcg tgccggttag
     2161 ccatcgcggt gccgttaccg ctggccccgc gacccgatgc ccgcatcgga gggggcgacc
     2221 gggccggtgc gccgggcatg gggccggtgg cgggtgacct tctccaccgg cccggccacg
     2281 aggttgcgat cgaccggcgc agcgggctcg acgcccggtc gcgactcgac gtacattacg
     2341 ggcgggaaga ggtcatccgc cggcccttcc cggcgttcaa cgatgcccag tttgccgaat
     2401 tccgtgacca tttcgttaat ccaatcgaga tagtgccctt tgaggccccg cagccaggtg
     2461 gcccgtgtat gaaaggcgcg catcctttcc tctatggaga gattcgggtt gcgggccctg
     2521 tcgtattcct cggcccgcaa gacgtggttc ggcacgcgcg gcggccagaa ggtgggcagg
     2581 tagggatcgt agtccttttc gtagcctgcg cggcaactgg cgctgtcggc ctgccagggc
     2641 acggccatcc agcgggtgat gtcgcccggc ccgttggcat agagcggccc attttcggcg
     2701 ttgatgctat ccggggtgag ctggtcgccg taatccggtt cgggctggcc gggctggcga
     2761 ggccggatgc gaaatggcgc gtaatacatg gtgacatggc gcatgggcca ggtcatttcg
     2821 cagccgggat ggaacgggcc gcccaggcag aattcgagcg cggcgcggtc cagcgtgtcc
     2881 ggctgctggg cgagcgggac gtcgtcgatc tccggcgcgg ccgccgcggt atcgggctcc
     2941 caatcggcga tgaagttgcc ctcggcccac tgctgcaagt acccgtattc ggtggtggtg
     3001 agcgccagga atgtgcgcgg cccgccggac ggcatgacga aatcgtcgcc atacataggg
     3061 ggccagccct tgacgtcgag cgtggtggcg gccgggtcgc ggaagaggtt gaagatctgc
     3121 cgccgcactt cccggtacgc ggcgtgcttg ctggccagtt gcgccaggta gtgtggccgc
     3181 agaaagtcgt agggcgcgcc ccagccgaac tggatgtgga agccgtagtt gacccaggcc
     3241 aggtcgcaga ggcggcgcag gatggggtag atgtgctgcg tgaaggacgg gcgcgtgatg
     3301 ggcgccagcc accagaactg gtaggcgtcg accatcacgc tgtacatggt ctgaatcgag
     3361 atcaggtcgg gcgcgtaatt gggcggcgcc accaccaccc aggccggatc gacggggatg
     3421 ctgcgcccgt tcagcgtgac ctgggcggtg acagggccgt cggagatgtc gtcatgccag
     3481 ccgttgttgt tgccgtaggt ggtcagcggg ttgtgcgtga acggcgtgcc ggactggccg
     3541 cgcccgccca ggaacagcag ccggccctgc gcgtcggtgc gcagttcgcc caggtagacg
     3601 gggtggccgc aaaaggtgcc ggtgtcgaac tgggccgcgg cgccgtcctg gtcgcgcccg
     3661 gtgaccgagc gcggaccagg gtcgatgatc agatcgccgc gctggtcaag gggataggcg
     3721 gcgttgcggc gggggcaggg gatcgcttcg gggatgtcga gcgccttctc gaagttgtac
     3781 cacgccgcct tcttgttggc gacatgcacc gtccacgtga tgtccgcgtc atcggccgtc
     3841 aattcctgca cgacggcgcc gttccggtca tagccgaaga ggcgaaaccg ggccgcctgg
     3901 cgcttcaggg caccggtcgc atccttgtac ccgcccgcgg gcggcggcat agcatagggc
     3961 acctccggtc cgatgaagta atcgccggcg ctgttgccga cgcgggcgat gccgatgccg
     4021 ggatggatca ccgcgcgcac aatctgtcga gctgcatcca tgggctgtct tcctcccact
     4081 gccgtggacc cctctgccgg tgcgagggcg ggggcgggac tcctggggcg gctgcggcgg
     4141 acatagccc
//
LOCUS       DASUYC010000203         2051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1598586, whole genome shotgun sequence.
ACCESSION   DASUYC010000203 DASUYC010000000
VERSION     DASUYC010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1598586"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VF276_03245"
     CDS             <1..231
                     /locus_tag="VF276_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605911.1"
                     /translation="GRGGVFFFDAQLKRAGQVRRNVFHALNPLRRPAAGQPDSAIARG
                     WVVPIAIAIEYNISSLIVPILGPACNGGLPDP"
     gene            250..897
                     /locus_tag="VF276_03250"
     CDS             250..897
                     /locus_tag="VF276_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6605912.1"
                     /translation="MTTEGTGPIRVLIADDHSVLREGLRMVIELQPDLEVAGEAKDGA
                     EALALAQQLQPDVVLLDLMMPQLDGLSALGQMKGVAPHARVVVLTSAEEEDRMIEAMR
                     AGAQGYLLKTEGASAVLDAIRAAYRGEVALAPRVARLLMHAVAQPASHPEDTLSPRER
                     EVLQLLAHGLSNKQIAAQLGMAEKTARAHVSSILQKLGFAGRTQAALYAREKGLV"
     gene            1120..1518
                     /locus_tag="VF276_03255"
     CDS             1120..1518
                     /locus_tag="VF276_03255"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6605913.1"
                     /translation="MADSVQRVRDAVRELIDLQSDMHVVGEAADGLTTLVLVEQLRPD
                     LILLDVRLEDGDGLRLAAHLRRTCPWLVLVVYTANPHDDTLEAAMRAGAARFVEKGAT
                     PQTLLHALREAAAARPLPLAGMPALEGDRT"
     gene            1515..>2051
                     /locus_tag="VF276_03260"
     CDS             1515..>2051
                     /locus_tag="VF276_03260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6605914.1"
                     /translation="MIASPMLDSVSFTNGAARTVPPTPGFLPAARPMTTGPSREAALI
                     AAAQSDPDAFADLYNLYYPAIYRFAQRHTGDPVQAEDVAAETFLAALKALPHYEWRGI
                     PFSAWLYRIASTVIAGQHRRNRGIAHVPLENPRFPLVEIACEEPGPDACIEAQEQQQI
                     ERHLLGIALKRLTDDQRRA"
BASE COUNT          340 a          689 c          670 g          352 t
ORIGIN      
        1 ggccgcggcg gtgtgttttt cttcgacgcg caactcaaac gtgccggtca ggtccggcgg
       61 aatgtctttc acgcactaaa tcctctccgc cgcccggcgg ccgggcagcc tgattcggcg
      121 atcgcacgag ggtgggtagt tcccatcgcg atcgctatcg agtataatat atccagcctg
      181 attgtaccca tccttgggcc ggcctgcaac ggcggcctgc ctgacccata aacgcagacg
      241 ggagcaccca tgacaacaga gggaacgggg ccgattcgcg tgttgattgc cgacgatcat
      301 tcggtgctgc gcgagggcct gcgcatggtg atcgagctac agcccgacct ggaggtcgcc
      361 ggggaagcca aggacggggc ggaggcgctg gccctggccc agcagttgca gcccgacgtg
      421 gtgctgctcg acctgatgat gccgcaacta gacggcctct cggcgctggg gcagatgaag
      481 ggcgtggcgc cccacgcccg cgtggttgtg ctgaccagtg cggaagaaga ggatcgcatg
      541 atcgaggcga tgcgggcggg cgcgcagggc tacctgctga agaccgaggg cgcctccgcc
      601 gtgctcgacg cgatccgcgc ggcctatcgc ggcgaggtgg cgctggcgcc ccgcgtggcg
      661 cgcctgctca tgcacgccgt ggcccagccg gccagccacc cggaggacac gctcagcccg
      721 cgcgagcgcg aagtcctgca actcctggcc catggcctca gcaacaagca gatcgccgcg
      781 cagctcggca tggctgaaaa gacggcgcgc gcccacgtca gcagcattct ccagaagctg
      841 ggcttcgccg ggcggaccca ggccgccctc tatgcgcgcg aaaagggcct cgtctaagcg
      901 tcctctgtgg gacatttgtc ccgccccccg ctaggcacat gaccgaccgc caggtcggtc
      961 attttgttat ggtcggatgc ccgttcgggc tgatcccagg gcggattaga acaggtacaa
     1021 tagcctcact tcttatgaac ggcattgttc tcatacttta gtcggagagg ttcttggaag
     1081 ctcatggacg cactacaccg ttcccccaca cgaattctgt tggctgacag tgtgcagcgg
     1141 gtgcgtgatg cggtgcgcga actcatcgat ttgcagagcg atatgcatgt cgtgggagaa
     1201 gccgcagatg ggttgactac cctggtgctg gtcgagcaat tgcgccccga cctgattttg
     1261 ctggatgtgc gcctggaaga cggggacggc ctgcgtctcg ccgcacatct gcgccgcacc
     1321 tgtccgtggc tggtgctggt agtctacacg gccaatccgc atgacgatac cctggaggcg
     1381 gccatgcgcg ccggggcggc tcgctttgtc gaaaagggcg ctactcctca gacacttttg
     1441 cacgcattac gggaggccgc cgcggcgcgg cccctgcccc tcgccggtat gccggcgctc
     1501 gaaggagacc gcacatgatc gccagtccga tgcttgattc cgtcagcttt acgaatggcg
     1561 cggcccgcac cgtgcccccg acccccggtt ttctcccggc tgcgcggccc atgacgaccg
     1621 gtccttcgcg ggaagccgcc ttgatcgcgg cggcccagtc ggatcccgat gccttcgccg
     1681 acctgtacaa tctctattac ccggccattt accgctttgc ccagcgccac accggcgacc
     1741 ccgtccaggc cgaggacgtg gcggcggaga ccttcctcgc ggcgctgaag gcgctgccgc
     1801 actacgagtg gcggggcatc ccgttcagcg cgtggctcta ccgcatcgcc tcgacggtca
     1861 tcgccgggca gcaccgccgc aaccggggca tcgcccatgt gccgctggag aacccgcgct
     1921 tcccgctggt ggagatcgcc tgcgaggaac cgggccccga cgcctgcatc gaggcccagg
     1981 aacagcagca gatcgagcgg cacctgctgg ggatcgcgct caagcggctg accgacgacc
     2041 agcgccgggc c
//
LOCUS       DASUYC010000204         2865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1701166, whole genome shotgun sequence.
ACCESSION   DASUYC010000204 DASUYC010000000
VERSION     DASUYC010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1701166"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="VF276_03265"
     CDS             <1..778
                     /locus_tag="VF276_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006714952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605915.1"
                     /translation="GHPLYSPYGLEATIPAMLLAHLFGASVVEALITGLGVAYLQKSH
                     PEYLTSLRSVFAGPEAVEGRAARRPLWQIVTGAAVVGAIGLFVAGLITGGGDIGHLFG
                     ADWAQVDWPAVATMLFIVAVIAVILIPLAYFVLPQRMKRVGTAFTALAVLAPLGLIAP
                     GFAYGEGSVEDVHAAFGYVPKGLQDLSSIFSAPLAGYNLPLPFFSHANAALWQTAIGY
                     EISGIVGILLVGGVMLALSALIRRRDSQRRTAPAQEAPQL"
     gene            775..1632
                     /gene="cbiQ"
                     /locus_tag="VF276_03270"
     CDS             775..1632
                     /gene="cbiQ"
                     /locus_tag="VF276_03270"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HEX6605916.1"
                     /translation="MSARAASAPAGRVGWLERTIADVAGSIERAVFTEEHARKPGWLQ
                     RIDPRAKLGMFVALVLAAGLSNALPVLTGLYLVILLAARASRVPFDFFVRRVWLGIPF
                     FAGVVILPSIFFAPGPRLFDLALGPLHLAPSIPGLTGAVVFVARVGVSVSLAVLLVLT
                     TPWADVLKSLQALRVPQVFILLLSMTYRYIFLFFRTANGMFEARKSRVVGRTSGNEQR
                     GWITGSMMSLLNRSFKMSNDVYAAMMARGFTGAIRSYTTYRMQTADWVALAGAVALAA
                     VALLAGRYL"
     gene            1632..2441
                     /locus_tag="VF276_03275"
     CDS             1632..2441
                     /locus_tag="VF276_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6605917.1"
                     /translation="MPDDLVFHLRGVRYRYGGRQLALDGIDLDIARGEQVVLLGANGS
                     GKSTLLKLLNGIYAPSEGTMRALGRDIQAVAAGMDAFQFHREVGLVFQDPDVQLFSAT
                     VFDDVAFGPLQLGLSQDEVKARCDEALQQMEITHLAQRAPFELSGGEKKRAAIASVLS
                     LRPDVILLDEPTASLDPRTKWVLVNLIRKLGAAGKTLITTTHELEIVPIIANRVVVIG
                     EERRILADGTPQEILSDHELLIKANLIHEHLIHDHLREPALAGEYARDGVR"
     gene            2564..>2865
                     /locus_tag="VF276_03280"
     CDS             2564..>2865
                     /locus_tag="VF276_03280"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEX6605918.1"
                     /translation="MSENRLTEYHEKLVASGVRLTPQRFMVLEVLAAHPGHITAEQVL
                     TAVQQRYPYVNKTTVYRTLELLTGLGLASMTHMGGNQYEYELLESPHHHLICKICG"
BASE COUNT          424 a          970 c          964 g          507 t
ORIGIN      
        1 cggccacccg ctctacagcc cctacggcct ggaggcgacg atcccggcga tgctgctggc
       61 ccacctgttc ggcgcgtcgg tggtcgaggc gctgatcacc gggctgggcg tggcctattt
      121 gcagaagtcg caccccgaat atctcacctc gctgcgtagc gtgttcgccg ggccggaggc
      181 cgtggagggc cgcgcggcgc gccgcccatt gtggcagatc gtcaccggcg cggcggtcgt
      241 gggcgcgatc gggttgtttg tggccgggct gatcaccggc ggcggcgaca tcggccacct
      301 gttcggtgcc gattgggcac aggtcgattg gcccgcggtc gccacgatgc tgttcattgt
      361 cgcggtgatt gcggtgatcc tgatcccgct cgcgtatttc gtgctgccgc agcgcatgaa
      421 gcgcgtgggt acggcgttca ccgcgctggc cgtgctggcc ccgctggggc tgatcgcacc
      481 ggggttcgcc tatggcgagg gcagcgtcga agatgtccat gccgcgttcg gctacgtgcc
      541 caagggcctg caagatctgt cgagcatttt cagcgcgccc ctggccggct ataacctgcc
      601 gctgcccttc ttcagccatg ccaacgccgc actctggcag acggccatcg gctacgagat
      661 cagcggcatc gtgggcatcc tgctggtggg cggggtcatg ctcgcgctga gcgccctgat
      721 ccggcggcgc gacagtcagc gccggacggc gcccgcccag gaggcgcccc aactatgagc
      781 gcccgcgccg catccgcccc cgcaggccgg gttggctggc tcgaacgcac catcgccgat
      841 gtggccggct cgattgagcg cgccgtcttc accgaggaac acgcgcgcaa accgggctgg
      901 ttgcagcgca tcgacccgcg ggccaagctg ggcatgttcg tcgcgctcgt gctggcggcg
      961 ggcctctcca atgccctgcc ggtgctgacc ggcctctacc tggttatctt gcttgcggcg
     1021 cgcgccagcc gggtgccgtt cgactttttc gtgcgccggg tctggctggg catcccgttc
     1081 ttcgccgggg tggtgatcct gccgtcgatc ttcttcgcgc cggggccgcg cctcttcgac
     1141 ctggccctgg ggccgctgca cctggccccc tcgatccccg gcctgaccgg cgccgtggtc
     1201 ttcgtggccc gcgtgggcgt gagcgtgtcg ctggccgtgt tgctggtgct gaccacgccc
     1261 tgggccgatg tgctcaagag cctgcaagcc ctgcgcgtgc cgcaggtgtt catcctgctg
     1321 ctgtcgatga cctaccgata tatcttcctc ttcttccgca ccgcgaacgg catgttcgaa
     1381 gcgcgcaaga gccgggtggt ggggcgcacc agcggcaacg agcagcgcgg ctggatcacc
     1441 ggctcgatga tgagcctgct caaccgctcc ttcaagatga gcaacgacgt gtacgcggcg
     1501 atgatggcgc gcggctttac cggggctatc cgcagctaca ccacctatcg tatgcagacc
     1561 gccgactggg tcgcgctggc cggggccgtg gccctggccg ccgtggcctt gctggcgggg
     1621 aggtatttgt aatgcccgac gatctggtct tccacctgcg cggggtgcgc taccgctacg
     1681 gcgggcgcca actggcgctc gacggcatcg acctcgacat cgcccgcggc gagcaggtcg
     1741 tcctgctggg cgccaacggc agcggcaaat cgacgctgct caagctgctg aacggcatct
     1801 acgcgcccag cgagggcacc atgcgcgcgc tgggccgcga catccaggcc gtggccgcgg
     1861 gtatggacgc cttccagttt caccgcgagg tggggctggt cttccaggac cccgacgtgc
     1921 aactgttcag cgccacggtg ttcgacgacg tggccttcgg ccccttgcag ttgggcctga
     1981 gccaggacga ggtgaaggcg cgctgtgacg aagcattgca gcagatggag atcacccacc
     2041 tggcccagcg cgcaccgttc gagttgtcgg gcggcgagaa gaagcgggcg gccatcgcct
     2101 cggtgctctc gctgcggccc gatgtgatcc tgctggacga gccgacggct tcgctcgatc
     2161 cgcgcaccaa gtgggtgctg gtcaatctga tccgcaagct gggcgcggcg ggcaagaccc
     2221 tgatcacgac cacgcacgag ctcgaaatcg tgccgattat cgccaaccgg gtcgtggtca
     2281 tcggcgagga acggcgcatc ctggccgacg gcacgccgca agagatcttg agcgatcacg
     2341 agctgttgat aaaggccaac ctgatccacg agcacctgat ccacgaccac ctgcgcgagc
     2401 cggcgctcgc gggcgaatac gcgcgcgacg gcgtgcgcta agccctgatt cgcggcatct
     2461 ttcgtgctgc cggctatccg cagcatgacg acgatgccgc ggcgcaggcg gtcgccggat
     2521 gtccatcccg ccggtccgcc gtagcggacc ctggattcaa gtcatgtctg agaatcgctt
     2581 aaccgaatat cacgagaaac tggttgccag cggtgtccgg ctgacgccac agcgcttcat
     2641 ggtgctggaa gtgctggcgg cccaccccgg ccatatcacc gccgagcagg tgctgaccgc
     2701 ggtccagcag cgctacccct atgtcaataa gacgacggtg taccgcacgc tcgaactgct
     2761 gaccggcctg gggctggcgt cgatgaccca catgggcggc aaccaatacg agtacgaact
     2821 gctggaatcg ccgcaccatc acctgatctg caaaatctgc ggcga
//
LOCUS       DASUYC010000205         2710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_887231,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000205 DASUYC010000000
VERSION     DASUYC010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887231"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..1098)
                     /gene="recA"
                     /locus_tag="VF276_03285"
     CDS             complement(34..1098)
                     /gene="recA"
                     /locus_tag="VF276_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX6605919.1"
                     /translation="MADREKAIETAIAQIERQFGKGSIMKMTEASEKIQVESIPTGAI
                     ALDLALGIGGIPRGRIIEIYGPESSGKTTLAQHIIAEAQKLGGTAAYIDAEHAMDPMY
                     AEKCGVNLETLLISQPDNGEQGLEIAETLVRSGAIDVIVVDSVAALTPRAEIEGEMGD
                     QHVGLQARLMSQALRKLAAAVSKSNTSLIFINQIRMKIGVMFGNPETTTGGNALKFYA
                     SVRMEIRRIESIKQGTDVVGNRVRVKVVKNKVAPPFRVAEFDIMFNEGISRIGNIVDI
                     GTELGILRKSGAFYSFGDQRLGQGREKVKEFLKQNPTIADDIEGRIRAAALAEAAPVV
                     LAAPANGNGAVADDEDLEEL"
     gene            complement(1259..1561)
                     /locus_tag="VF276_03290"
     CDS             complement(1259..1561)
                     /locus_tag="VF276_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605920.1"
                     /translation="MQLKTVHEGKVGAEVTPQKYALVRAAILAALPAAGTGLTWDELV
                     QAIAPGLPPHLFPHLGSVRWYTKAVQLDLEARGLIARVPKTRPLRLLRRPDVVPSS"
     gene            complement(1631..2023)
                     /locus_tag="VF276_03295"
     CDS             complement(1631..2023)
                     /locus_tag="VF276_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6605921.1"
                     /translation="MISHISSVTVFVRDQDQALDFYVNKLGFQKTADEPMGEGMRWVT
                     VAPPGAATAIVLAHGYGGYDEARIGQFAGVVFTTDDIQATYQELSGRGVHFTEPPALQ
                     PWGMMQAIFDDQDGTHFVLVQPPGGGQG"
     gene            complement(2237..>2710)
                     /locus_tag="VF276_03300"
     CDS             complement(2237..>2710)
                     /locus_tag="VF276_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEX6605922.1"
                     /translation="LWRDYQNVGVVLQAYLYRTPQDVDEMIRLGARVRLCKGAYNEPP
                     SVAFPEKKDTDMQYARLMCKLIDLGNYPGIATHDVRLINLAKRFVAKKGIPSDRFEFQ
                     MLYGVRSQVQRDLTAEGFNMRVYVPYGEQWYPYFMRRLAERPANLIFIMSNLLRK"
BASE COUNT          437 a          909 c          848 g          516 t
ORIGIN      
        1 tcggctgctg tcagctaagc agatgccggc gcgctaaagc tcttccagat cctcgtcgtc
       61 ggccactgcg ccgttgccgt tggccggggc ggccagcacg acgggcgcgg cctcggccag
      121 cgcggcggcg cggatgcgac cctcgatgtc gtcggcgatg gtcggattct gcttcaggaa
      181 ctccttgacc ttttcgcggc cctggcccag gcgctggtcg ccgaagctgt agaaggcgcc
      241 gctcttgcgc aggatgccca gctccgtgcc gatgtccacg atgttgccga tgcggctgat
      301 gccctcgttg aacatgatgt cgaactccgc gacccggaac ggcggggcca ccttgttctt
      361 gaccaccttc acgcgcaccc ggttgccgac cacgtcggtg ccctgcttga tcgactcgat
      421 gcggcggatc tccatgcgca ccgacgcgta gaacttgagc gcgttgccgc cggtcgtcgt
      481 ctccgggttg ccgaacatga cgccgatctt catgcggatc tggttgatga agatgaggga
      541 cgtgttgctc ttgctcacgg cggcggccag cttgcgcagc gcctggctca tcaggcgcgc
      601 ctgcaaacca acgtgctggt cgcccatctc gccctcgatc tcagcgcggg gagtcagcgc
      661 ggccaccgag tcgaccacga tcacgtcgat ggcgcccgag cggacgagcg tctcggcgat
      721 ctccaggccc tgctcgccgt tatccggctg cgagatcagc agcgtttcga ggttgacgcc
      781 gcacttctcg gcgtacatcg ggtccatcgc gtgctcggcg tcgatgtagg ccgccgtgcc
      841 gcccagcttc tgcgcctcgg cgatgatgtg ctgggccagg gtcgtcttgc ccgagctttc
      901 cgggccgtag atctcgatga tccggccccg cgggatgccg ccgatgccca gcgccaggtc
      961 cagcgcgatg gcgccggtgg ggatcgactc cacctggatc ttctcgctgg cctcggtcat
     1021 cttcatgatc gagcctttgc cgaactgccg ctcgatctgc gcgatggcgg tctcaattgc
     1081 tttttcacga tctgccatgg tctcgttgct ccttctgcat ctctatctca gcttggtaca
     1141 caatcgggga ttggagtccg cctccgggca ggagcgggaa ccggtcgcga aatacgacca
     1201 tttgttctac tcacatctct aatcataccc gattcgcgac cgtttgtcaa ctaatttatc
     1261 aacttgacgg gaccacatct ggccggcgca gcaagcgcaa gggccgggtt ttgggcaccc
     1321 gcgcgatcag gccgcgcgcc tccaggtcga gttgcaccgc cttggtgtac cagcgcaccg
     1381 agcccaggtg cgggaacagg tgtggcggca ggcccggcgc gatggcctgc accagttcgt
     1441 cccaggtcag gccggtgccg gcggcgggga gggcggccag gatcgccgcg cgcaccagcg
     1501 cgtacttctg cggcgtcacc tccgcgccca ccttgccctc gtgcaccgtc tttagctgca
     1561 taacggttcc tcccgctagc ctcgcagcgc cgctggaggg gccggtatgt cgcccgccct
     1621 gcggctccgc ttaaccctgc ccaccgcccg gcggctgcac cagaacgaag tgggtgccat
     1681 cctggtcgtc gaagatggcc tgcatcatgc cccagggctg gagggcaggc ggctcggtga
     1741 aatggacacc gcgcccgctc agttcctggt aggtcgcctg gatatcgtcg gtcgtgaaga
     1801 caaccccggc gaactggccg atgcgcgcct cgtcatagcc gccgtagccg tgggccagca
     1861 caatcgccgt cgcggcgccc ggcggcgcca ccgtcaccca gcgcatgcct tcgcccatgg
     1921 gctcgtcggc ggtcttctgg aagcccaatt tgttgacata aaaatccagc gcctggtcct
     1981 gatcgcggac aaagacggtc acggaactga tatgcgagat catcgggcta cctcctcagc
     2041 cggaacggcg cctggggcgt ggtgtgcgca cgccccatcg ctcccgttct ctctacctat
     2101 cgccacgccg cggaaaaagc ggaaagcggc cagcccccaa tattggaaac tgaccgctga
     2161 ctccgactct tgcctcctgc ccaccaacgt actgacggct gctcgcgtag cggtgccgca
     2221 ggtacacggc tgatagctac ttgcggagca agttactcat aatgaagatc aaattcgccg
     2281 ggcgctcggc caggcggcgc ataaagtagg ggtaccactg ctcgccgtag ggcacgtaga
     2341 cacgcatatt gaagccctcg gcggtcaggt cgcgctggac ctggctgcgc acgccgtaga
     2401 gcatctggaa ctcgaaccgg tcggacggga tgcccttctt ggcgacgaag cgcttggcga
     2461 ggttgatcag gcgcacgtcg tgcgtggcga tgcccggata gttgcccaga tcgatcagct
     2521 tgcacatcaa gcgcgcgtac tgcatatcgg tgtccttttt ctcagggaag gcgaccgagg
     2581 gtggctcgtt ataggcgccc ttgcagagcc gcacgcgcgc acccaggcgg atcatctcgt
     2641 ccacatcctg cggcgtgcga tacagatagg cctgcaacac cacgcccaca ttctggtaat
     2701 cgcgccagag
//
LOCUS       DASUYC010000206         2716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_438683,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000206 DASUYC010000000
VERSION     DASUYC010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438683"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VF276_03305"
     CDS             <1..200
                     /locus_tag="VF276_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605923.1"
                     /translation="DGTLRSGTVQGSIHAVGRALAGLPSCNGWDAWYYDDPATGERQV
                     LDTLRRRIRLGGTEPLRAAGE"
     gene            complement(374..1870)
                     /locus_tag="VF276_03310"
     CDS             complement(374..1870)
                     /locus_tag="VF276_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX6605924.1"
                     /translation="MSEVLFGQSYYLRFDPKEWATMMPYPPLGTLYAASYLRAQGYDV
                     ALFDSMLAESEDEWTAALDAHRPRFAVIYEDSFNYLSKMCLTRMREAAFRMIPAAKAR
                     GCYVIISGSDATDHQEKYFAAGADAIVLGEGEKTLAELLDCLTGREEGDPAAVVGVST
                     PAAPSHTKRDFIRALDDLPFPAWDLVDIAKYKAIWHEHHGYYSMNLVTTRGCPFHCNW
                     CAKPIYGQRYNVRSPESVAAEVRWLKANFAPDHIWFADDIFGLKPNWIPKFADEIERL
                     DARIPFKCLLRVDLIKEGVPEALKRAGCDIVWVGAESGAQKVLDAMDKGTKVEQIYAA
                     AKNLHRVGIRIGFFLQFGYPGEVRADIEKTLQMVRDCNPDDIGISVSYPLPGTRFYDR
                     VQAQLGTKRNWVNSQDLDLMFQGAYVPEFYRTLYSVVHHEFRMRKARDRAVPALRRPW
                     TLRPTHARALAAIPRHWAGLQVRRAQLNRLERVKRPDDPVIPLMPVSG"
     gene            complement(2234..>2716)
                     /locus_tag="VF276_03315"
     CDS             complement(2234..>2716)
                     /locus_tag="VF276_03315"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX6605925.1"
                     /translation="EMIRSQRYDRPLSCIMIDVDHFKRVNDTYGHQVGDQVLLDLARR
                     LNRLARSNDVFARYGGEEFTLLLPETGLAEAVQTAERIRQRIAGAPFLVTPAPDSPPL
                     AVHLTASLGVCTREASVRDGAQLLRDADQALYRAKGAGRNCVMYTHRPSLDENLRVSR
                     "
BASE COUNT          435 a          864 c          948 g          469 t
ORIGIN      
        1 ccgacggcac gctgcgcagc ggcacggtgc aggggtcgat ccatgccgtg ggccgggcgc
       61 tggcgggcct gccctcgtgc aacggctggg acgcgtggta ctacgacgac cccgccaccg
      121 gtgagcggca ggtgctcgat acgttgcgcc ggcggatccg gctcggcggc accgaaccgt
      181 tgcgcgccgc cggggaatag ggcggcgggc atggcgtggg ccggtttgaa accgacccct
      241 acagcggcca gcagcggtga cgcccggata tacggcgcgg gccggtttga aaccggcccc
      301 tacaatagcc aaccggcgcg atgatcggat atacggcgcg ggccggtttc aaaccggccc
      361 gcgtcttgtc cagctagccg gaaaccggca ttagcgggat caccgggtcg tcggggcgct
      421 tgacgcgctc caggcggttc agttgggcgc ggcgcacttg caggcccgcc cagtggcggg
      481 ggatggccgc cagggcgcgg gcgtgggtgg ggcgcagcgt ccagggccgc cgcagcgccg
      541 ggaccgcgcg atcccgcgcc ttgcgcatgc ggaactcgtg gtggacgacg ctgtagagcg
      601 tgcggtagaa ctcgggcacg taggcgccct ggaacatcag gtcgaggtcc tggctgttca
      661 cccagttgcg cttggtgccc agttgcgcct gcacccggtc gtagaagcgg gtaccgggca
      721 gcgggtagga gaccgagatg ccgatgtcgt cggggttgca gtcgcgaacc atctgcagcg
      781 tcttctcgat gtcggcgcgc acctcgccgg ggtagccgaa ctgcaggaag aagccgatgc
      841 ggatgcccac ccggtgcagg tttttggccg ccgcgtagat ctgttcgacc ttggtgccct
      901 tgtccatagc gtcgagcacc ttctgcgcgc cgctctcggc gcccacccag acgatgtcgc
      961 agcccgctcg cttcaacgcc tcgggtaccc cctccttgat caggtccacg cgcagcaggc
     1021 acttgaaggg gatgcgcgcg tccagccgct cgatctcgtc ggcgaatttc gggatccagt
     1081 tgggcttgag gccgaaaata tcgtcggcga accagatgtg atcgggcgcg aagttggctt
     1141 tgagccagcg cacctcggcg gccacgcttt cggggctgcg cacattgtag cgctggccgt
     1201 agatcggctt ggcgcaccag ttgcaatgga acgggcagcc gcgcgtcgtc accaggttca
     1261 tcgaatagta gccgtggtgc tcgtgccaga tcgctttgta cttcgcaata tcgaccaggt
     1321 cccaggcggg gaagggcagg tcgtcgagcg cgcggatgaa atcgcgcttg gtgtggctgg
     1381 gcgccgccgg tgtcgacacg ccgaccaccg ccgccgggtc gccttcctcg cggccggtca
     1441 ggcagtcgag cagttcggcc agggtcttct cgccctcgcc cagcacgatg gcatcggccc
     1501 cggcggcgaa atacttctcc tggtggtctg tggcatcgga gccggagata atgacgtagc
     1561 agccacgggc cttggccgcc gggatcatgc ggaaggcggc ctcgcgcatg cgcgtcaggc
     1621 acatcttgct caagtagttg aagctgtcct cgtagatgac ggcgaagcgg gggcggtggg
     1681 catcaagggc tgccgtccac tcgtcttcgc tctcggcgag catgctgtcg aacagcgcca
     1741 catcgtaacc ctgcgcgcgc aggtacgagg cggcgtaaag ggtgccgagg ggcgggtacg
     1801 gcatcatggt cgcccactct ttggggtcga agcgtaagta gtacgactga ccgaacaaca
     1861 cttcgctcat agtggtactc cttcctgcgc tgggccattc ccgcgattat agtgcgatcc
     1921 cggcgtggcg tcaatgtatc gcggcctgcc atccggccat gcaccctgat tctgcgccgt
     1981 gcagacacga aggcgcgaaa aacaagacgg acataaagcg gcttcgcggc gaacttatca
     2041 ccggctgcga ggcagcggca ccgattggcg cccgtggccc acattcctgc taacgccgcg
     2101 cccgcgcgaa aagtagcggg gcgcccccct agcgcgaggt cgagtggcct gttaggtgta
     2161 gttgcgcgat ttatcgcgcc gtactcgttg gttgttagcg tattcggtcc ccaacaggtc
     2221 gctggaacct gcgctagcgc gagacacgca gattttcgtc cagtgagggg cggtgcgtat
     2281 acatgacgca gttccggcct gcacctttgg cccggtacag ggcctggtcg gcgtcgcgca
     2341 gcagttgggc gccgtcgcgc accgaggctt cgcgcgtgca gacgcccagg ctggccgtca
     2401 ggtgcacggc gagcggcggg ctgtcggggg ccggggtgac aaggaaaggc gccccggcga
     2461 tgcgctggcg gatgcgctca gccgtttgca cggcctcggc cagccctgtc tccggcagca
     2521 acagggtaaa ctcctcgccg ccgtagcgcg cgaacacgtc gttgctgcgc gccagccggt
     2581 tcaagcgccg ggccaggtcc agcaacacct ggtcgcccac ctggtgccca taggtgtcgt
     2641 tgacccgctt gaaatggtcc acatcgatca tgatgcaact cagcgggcga tcatagcgct
     2701 ggctgcggat catttc
//
LOCUS       DASUYC010000207         4130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_774380,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000207 DASUYC010000000
VERSION     DASUYC010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774380"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /locus_tag="VF276_03320"
     CDS             <1..1143
                     /locus_tag="VF276_03320"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6605926.1"
                     /translation="DSQTGGEIGALSGEFNAMANRLSDTIRRRTTERNQMAAVLAHMH
                     DGIVLTDAHGAIDGINPTACRLLGTTPEHAVGRSLVALTQSHEMYQAGRAALAAPGER
                     QYLELTLGPYQVACVVTGIPGPAGAPPSGLAVLQDVTEVRRLERVRRDFVANIGHELR
                     TPLASIKLLVETLHTAIHDDPEAADDFLRSIDVELDGLTQLVRELLELSRIESGQVQL
                     DRHPVPVDTLLERAAARLRAQAERAGLTLTVRPAPTLPPADVDATRIEQVLVNLVHNA
                     IKFTPPGGEVTLGAEPAPAGVRLYVRDTGIGIAPAEQPRLFERFYKVDKARAAGAASA
                     GGTGLGLAIAKHLVQAHGGEIGVISRPGAGATFAFTLPAARVLSAE"
     gene            complement(1338..1766)
                     /locus_tag="VF276_03325"
     CDS             complement(1338..1766)
                     /locus_tag="VF276_03325"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX6605927.1"
                     /translation="MVCKNCGKELPLAASFCWQCGTSLRAADGPAREDPAPAWETCEV
                     MWEVVQEGKPALVGSPTHLLRFWARAYGARGTYMAGETEPFSVKKAGSIAYPIPEREA
                     VTVHGTFIQQLIDEGWEPIRDEAETWFHTHLRRRVRSPRR"
     gene            2372..2959
                     /locus_tag="VF276_03330"
     CDS             2372..2959
                     /locus_tag="VF276_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX6605928.1"
                     /translation="MTLTSSEQNTQSTANAAAAPVYQGWRVDPMHTIVQFAVKHMMIS
                     TVRGGFHSPNGTILVDEQDLTRSRVEAVIDARTLDTHAEERDVHLRSADFLDVENHPQ
                     ITFTSTRVEVLDPEHVRVLGNLTIRGVTQEVPLDVELGGRNVTPWGEQKMGFSATTKI
                     NRKDFGLNWNMALEMGGMMVGDDIKISIDGEATPL"
     gene            3254..4030
                     /locus_tag="VF276_03335"
     CDS             3254..4030
                     /locus_tag="VF276_03335"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6605929.1"
                     /translation="MVPATQQISNPRTLPAAMQVRPSLPAPFDPAPPAGDGISAALLD
                     RAQQRRAAGDLLGALRDLDACLATRPDHAPALVLRAWVLRDLGRVHAAAFDFDRAVAL
                     APHDPDVLYNRALFHEESGDMSRAAVDWLQMQALRRESGAAAPAPPALRPHRPLYNRP
                     AHLQAPPAAPAPIDELYRRGIQYVEMGNPPAGIGALLQVLDYRPADPDVLYRLAWAWA
                     CAGDYGEALQWLGAALAIRPALAALADQDPAFAPCRLPEL"
BASE COUNT          673 a         1470 c         1331 g          656 t
ORIGIN      
        1 gacagccaga ccggcggcga gatcggggcg ctgagcggcg agttcaatgc catggcgaac
       61 cggctgagcg acaccatccg ccgccgcacg accgagcgca accagatggc cgctgtgctg
      121 gcccatatgc atgacggcat cgtgctcacc gacgcccacg gcgccatcga cggcatcaac
      181 cccactgcct gccgcctgct cggcaccacg ccggagcatg ccgtgggccg gtcgctggtc
      241 gcgctgaccc agagccacga gatgtaccag gcggggcgcg ccgccctggc cgcgccgggc
      301 gagcggcaat acctggagct gaccctcggc ccctatcagg tggcctgcgt cgtcaccggc
      361 atccccggcc cggcaggcgc cccgcccagc ggcctggccg tgctgcaaga tgtgaccgag
      421 gtgcgccgcc tggagcgcgt gcgccgtgac ttcgtggcca atatcggcca cgagctccgc
      481 acgccgctgg cctcgatcaa gctgctggtg gagacgctgc acacggccat ccacgacgac
      541 cccgaggcgg cggacgactt cttgcggagc atcgacgtgg aactggatgg gctgacccaa
      601 ctggtgcgcg aactgctgga actgtcgcgc atcgagtccg ggcaggtgca actggaccgc
      661 cacccggtgc cggtggacac gctgctggag cgggccgccg cacggctgcg cgcccaggcc
      721 gagcgcgccg gcctcaccct gaccgtgcgc cccgccccga ccctgccgcc cgccgatgtg
      781 gacgccacgc gcatcgagca ggtgctggtc aatctggtcc acaacgcgat caagttcacg
      841 ccgccgggtg gggaggtcac gctcggggcc gagccggctc cggcgggcgt gcggctctac
      901 gtgcgcgaca ccggcatcgg catcgcgccg gctgagcagc cgcggctgtt cgagcggttc
      961 tacaaagtgg acaaagcgcg cgccgcgggg gccgcgagcg cggggggcac ggggttgggc
     1021 ctcgccatcg ccaagcacct ggtgcaggcc cacggcggcg agatcggcgt gatcagccgt
     1081 cctggtgcgg gcgccacctt cgccttcacg ctgccggccg cacgagtgct gagtgctgag
     1141 tagggacgag tggtcagtgg tcagtggtca gtaatgaggc ttgtcccgct gagcgcagcg
     1201 aagagtctcg tctcgcgcag gaggcttgtc actctgagcg cagcgaagag tctcgtcccg
     1261 tagccacgga gacgagatgc ttcactgcgt tcagcatgac aagtgaggcc ggcagcagag
     1321 gtaaggcggc gatgttgcta gcgccgcggc gatctcaccc ggcggcgcag gtgcgtgtgg
     1381 aaccaggttt ccgcctcgtc gcggatcggt tcccagccct cgtcgatcaa ttgctggatg
     1441 aaggtgccgt gcaccgtgac ggcctcgcgc tcggggatgg gataggcaat cgaccccgcc
     1501 tttttcacgc tgaacggttc cgtctccccc gccatatagg tgcccctggc cccataggcg
     1561 cgcgcccaga agcgcagcag atgcgtgggg gaaccgacca gggccggctt gccttcctgg
     1621 acgacctccc acatcacttc gcaggtctcc caggcggggg cggggtcttc gcgcgccggg
     1681 ccgtcggcgg cacgcagcga cgtgccgcac tgccagcaaa aactcgccgc caacgggagt
     1741 tccttgccac agtttttgca gaccataacg aacctcctgg tggagagtcg gatggaggcc
     1801 acggcagtgg ggccggcatc tgggtacagg atcggacata tggggtatgc gaatcccaga
     1861 attgtcatgg gcccggttgg ggcgccagga agcttaccaa actgcttggt cgccggtcat
     1921 cctgttgtgc cgtgccgatt aactcggtgg ccgccacggg tgcgccccat agcaaactct
     1981 cagcggcaat ggtagccgga gcgtcgggtg cggacggcgc tgagaaaggt tcagctatta
     2041 tgatggtttg tattgtagca gaatcccccc cgcaatgcaa gtgcccggtc aggttttggt
     2101 aaggtaaagg gcggctgcgc ggtaaggtca gcgctgtacc cctccgcggc gcaccgcctg
     2161 gccgcggaaa agtcaagcgc gaggcgccaa tagacccatt gcagtattgc gaatctatgt
     2221 tatactattc tcagtgagac ctcgctgtgc gttcaccagc actcgcttgc catctcctcc
     2281 gctttctttc gcgccgcgtg tgcctgatcc cctgtgttgt ctctggagca gcaactggat
     2341 tcgtatgggc gcataaggag ccggcaattc tatgactctt acttcgagcg agcagaacac
     2401 gcaaagcacc gcgaacgccg ccgcggcccc tgtttaccag ggctggcggg tcgatcccat
     2461 gcacacgatt gtgcagttcg ccgtgaagca catgatgatc tccaccgtgc gcggcggctt
     2521 ccactcgccc aatgggacca tcctggtcga tgaacaggac ctgacccgct cgcgcgtgga
     2581 ggcggtcatc gacgcgcgca cgctggatac gcacgccgag gagcgcgatg tgcatttgcg
     2641 cagcgccgat ttcctggacg tggagaacca cccgcagatt accttcacga gcacacgcgt
     2701 cgaggtcctt gaccccgagc atgtccgcgt cctaggcaat ctgaccatcc gcggcgtgac
     2761 ccaggaggtg cccctggacg tggaactggg tgggcgcaac gtgaccccct ggggcgagca
     2821 gaaaatgggc ttctccgcga ccaccaagat caaccgcaag gactttggcc tgaattggaa
     2881 tatggccctg gagatgggcg gcatgatggt cggcgacgac atcaagatca gcatcgacgg
     2941 cgaagccacg ccgctctaag cacccggcgt gcccccgaac ccacgcgcgt aggagccggt
     3001 tcgggggcac gtcaccgttg tacggccaaa ccgcctcgcc cgcctgcacg aacgcgaccg
     3061 ctgccccacg gcgcccccca tccccacgac cgctcccgcc caattcatag caattgttta
     3121 cattcctcta acccgcgctt aatcttccgg cgcgataata gtcgtgtagc aaccacaaac
     3181 ataacgccgc cactgccggc ggcaacaaga atccacgcac gcttgccgat ccgcgcgatc
     3241 ggggaggttt cagatggtgc ccgccacaca gcagatcagc aacccacgca cgttacccgc
     3301 cgcgatgcag gtccggccca gccttccggc gccgttcgac cccgcgccgc cagcaggcga
     3361 cggcatatcc gccgccctcc tggaccgcgc gcagcagcgg cgcgctgcgg gcgatctgct
     3421 cggcgccttg cgcgacctgg acgcctgcct ggcgacccga cccgatcacg ccccggccct
     3481 cgtgctgcgc gcctgggtgc tgcgcgacct ggggcgggtg catgccgccg cctttgactt
     3541 cgaccgcgcc gtggccctcg cccctcacga tcccgacgtg ctctacaacc gggcactctt
     3601 ccacgaggaa tccggggata tgtcccgcgc cgccgtcgat tggctgcaga tgcaggcgtt
     3661 gcgtcgcgag tccggtgccg ccgcgccggc cccacccgcg ctgcggcccc accggccgct
     3721 gtataaccgg cccgcgcatc tgcaggcgcc gcccgccgcc ccggcgccca tcgacgaact
     3781 ctaccggcgc ggcatccagt atgtcgagat gggcaacccg cccgccggca tcggcgcgct
     3841 cctgcaggtg ctcgactacc gccccgccga ccccgacgtg ctctaccgcc tggcctgggc
     3901 ctgggcttgc gcgggcgact acggcgaggc gctgcaatgg ctcggcgccg ccctggccat
     3961 ccgccccgcg ctggccgccc tggccgacca ggacccggcc ttcgccccct gccgcctgcc
     4021 ggagctgtag cgacccggcc tctctccggc catcttgccg ctgtaggggc gggtttcaac
     4081 cccgcctgct gccccaccac cccgctctct caccagccgt cccgctgctg
//
LOCUS       DASUYC010000208         3882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1206921, whole genome shotgun sequence.
ACCESSION   DASUYC010000208 DASUYC010000000
VERSION     DASUYC010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1206921"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..1466)
                     /gene="asnS"
                     /locus_tag="VF276_03340"
     CDS             complement(126..1466)
                     /gene="asnS"
                     /locus_tag="VF276_03340"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEX6605930.1"
                     /translation="MSDTEVKEEAAPAAAAPVVYIRDLPDHVGQEVTVRGWVQHHRSK
                     GKLQFIVMRDGTGVCQLVAFKGDLSAEDWEAADKLTLESAFAATGTVRADERAPGGVE
                     VSVRHVAVYQYTQDYPIAEKEHGVAFLMENRHLWLRSSRQNAALKLRAGVIKAVRDYL
                     DSHDFTLVDAPILTPSAAEGTSTLFEIDYFGQPAFLSQSGQLYNEAAIGSLGKVYCFG
                     PTFRAEKSKTRRHLMEFWMVEPEVAFMELPGLLELEEQFVSYIVQTVLDTHKEELKVL
                     ERDTSKLEKIVPPFPRVTYDEAIAYLQRTVNPDAKWGDDFGGDEETALGSQYDKPLFI
                     THYPTAIKAFYMQPDPQRPDVVLAADLIAPEGYGEICGGSQRIHDLALLEERMRAHNL
                     PREAYEWYLDVRRYGSVPHSGFGMGIERVTAWIGGLPHVRETIPFPRMYEWCRP"
     gene            complement(1539..3755)
                     /locus_tag="VF276_03345"
     CDS             complement(1539..3755)
                     /locus_tag="VF276_03345"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6605931.1"
                     /translation="MSPDSAGRRTGAPAPEPAAVPDLPAGAVDTLLAFSEAVARSTRP
                     DDLLARITAAAVALVGLPWSGVVLRDPHTDELRLVATEGVPLAALPPVLEFSLESRLY
                     QTLRRGELFIVEDYAAATSQTLVGRILARGIGANTMLAAPLQIDGDFLGAIYLAGPAP
                     HHYTPLEQALIRRLARLAAQLLVHTRALSAAEQRAGALETMQALATSLEATLDLETVL
                     RMLIGQSVDLVGASGGALILWDEDSGKLVTRMSSGTAYDYPPVAPGQGLTGTAFTTGR
                     PQVQNSYQHFAGAIPRASDQHVTNALAVPLLRGGRPFGTITVFSMIFKPFTPEDIRTL
                     TLFASQAAVAIDNQMLYSTVLSQQRQLDSIIRAMHDALVVYDRAGTIILINPAAEDLI
                     GLRAAVVGVSRDELMRNYERYAKYQVEPLHDWERTFETVLTTGVTLAAMSHVHSDRVR
                     TVESIFAPYLDAEGAIIGVISVTRDVSVSQELERVRAELEVARRQEDFLSIAAHELRT
                     PITAIKGYADLLNRRMAPGYTVAERDTRMLGSIVAQVSRLTELIDDLLDMGRIQAGRV
                     EFHWERCSIRAVLRGAIDSVRPVAGAREGDLQLVGEDDGLVRCDPGRLEQVFVNLLTN
                     ALKYSTAGPVTCTITRLHDRVRVAVQDRGAGIPPDDLPRLFERFFRAHDVYAHQRGLG
                     LGLYISRQIVERHDGRIWAESELDAGSTFYVELPLADGNSASDGAASAAPEALAGS"
BASE COUNT          631 a         1302 c         1320 g          629 t
ORIGIN      
        1 gactgccgtc tgtaccaccc ggcatcaacc ttgatgtcga actaaaggca gaaagcgtgc
       61 cagggccgcc cgcgcgaacg ggcggccctg gcaccacatg attattcgag ttagctataa
      121 tccggtcagg gccggcacca ctcgtacatg cgcgggaagg ggatggtttc gcggacgtgg
      181 ggcagcccgc cgatccaggc ggtgacgcgc tcgatgccca tgccgaagcc cgagtggggc
      241 acgctgccgt agcggcgcac atccaggtac cactcatacg cctcgcgcgg caggttgtgc
      301 gcgcgcatgc gctcttccag cagcgccagg tcgtggatgc gctggctgcc gccgcagatc
      361 tcgccgtagc cctccggggc gatcaggtcg gcggccagca ccacgtcggg ccgctgggga
      421 tcgggctgca tatagaaggc tttgatcgcc gtcgggtagt gggtgatgaa cagcggcttg
      481 tcgtactggc tgcccagcgc cgtctcctcg tcgccgccga agtcgtcgcc ccacttcgcg
      541 tcggggttca ccgtgcgctg caaataggcg atggcctcgt cgtaggtgac gcgcgggaag
      601 ggcggcacga tcttttccag cttgctggta tcgcgctcca gcaccttgag ttcctctttg
      661 tgcgtgtcta gcaccgtctg gacaatatag gagacaaact gctcctctaa ttccagcagg
      721 ccgggcaact ccataaacgc cacctcgggc tcgaccatcc agaactccat caggtgacgg
      781 cgcgtcttcg acttctcggc gcggaaggtg gggccgaagc aatagacctt gcccaggctg
      841 ccgatggcgg cctcgttgta aagctggccg ctctggctca ggaaagcggg ctggccgaag
      901 tagtcgatct cgaacagcgt cgaggtcccc tcggcggcgc ttggcgtcag gatcggcgca
      961 tcgaccagcg tgaagtcgtg gctgtcgaga tagtcgcgca cggccttgat caccccggcg
     1021 cggagcttca gcgcggcgtt ctgccgcgac gagcgcagcc agaggtggcg gttttccatc
     1081 aggaatgcga cgccgtgctc tttctcggcg atggggtagt cttgcgtgta ctggtagacc
     1141 gcgacatgcc gcaccgagac ttcgacgccg cccggcgcgc gctcgtcggc gcgcacggtg
     1201 ccggtggcag cgaaggccga ctccagcgtc agcttatcgg cggcctccca gtcctcggcg
     1261 ctcaggtcgc ccttgaaggc gacaagctgg cagacaccgg tgccgtcgcg catgacgata
     1321 aattgcagct tgcccttcga ccggtggtgc tgcacccagc cgcgcacggt gacttcctgc
     1381 cccacatggt ccggcagatc gcggatatag acgacggggg ctgccgccgc gggcgcggcc
     1441 tcttccttca cttcagtgtc cgacatggct ctctcctcca gacccgttct cgccgtgtgc
     1501 ggaacgcggc agtgtactat accacaaccg ggccgggctt agctacccgc aagcgcctcg
     1561 ggcgccgccg aggcggcgcc gtcgctcgcg ctattgccat cggccagggg cagttcgaca
     1621 tagaacgtgc tgcccgcgtc caactccgac tcggcccaga tgcgcccgtc gtggcgttcg
     1681 acgatctggc ggctgatgta gaggccgagg cccagcccgc gctggtgggc atagacatcg
     1741 tgcgcgcgga agaagcgctc gaacagccgg ggcagatcgt ccggcggaat gcccgcgccg
     1801 cgatcctgca cggccacccg gacccggtca tggaggcgcg taatggtgca ggtcaccggc
     1861 cccgccgtcg aatacttgag cgcgttggtc agcaggttga cgaaaacctg ctccaggcgc
     1921 cccggatcgc agcgcaccag cccatcgtcc tcgcccacga gttgcaaatc gccctcgcgg
     1981 gcgccggcca ccggccgcac gctgtcgatg gccccgcgca gcaccgcgcg gatagagcac
     2041 cgctcccagt gaaactccac ccgcccggcc tggatgcggc ccatgtcgag cagatcgtcg
     2101 atcagttcgg tgaggcggct cacctgggcc acgatggacc ccagcatccg ggtgtcgcgc
     2161 tcggcaacgg tgtagccggg ggccatgcgg cggttcaaca ggtcggcata gcccttgatc
     2221 gccgtgatcg gcgtgcgcaa ctcgtgggcg gcaatcgaga gaaaatcctc ttgccggcgc
     2281 gccacttcca attcggcgcg tacgcgctcc aactcctgcg agacactgac atcgcgcgtc
     2341 accgaaatca cgccgatgat cgcgccctcg gcgtccaggt acggggcgaa gatgctctcg
     2401 acggtccgca cccggtcgct atgcacgtgg gacatcgcgg ccagcgtgac gccggtcgtc
     2461 agcaccgtct cgaaggtccg ctcccaatca tgcagcggtt ccacctggta tttcgcgtag
     2521 cgctcataat tgcgcataag ctcgtcgcgc gacacgccga cgacggcggc ccgcaggccg
     2581 atgagatctt ccgccgccgg gttgatcaga atgatcgtgc ccgcgcggtc gtagacaacc
     2641 agcgcgtcgt gcattgcccg gatgatgctg tcgagctggc gctgctggct gagcaccgtg
     2701 ctgtagagca tctggttgtc gatggcgacg gcggcctggc tggcgaagag ggtcagcgtg
     2761 cggatgtctt cgggcgtgaa gggcttgaag atcatgctga acacggtaat cgtgccgaag
     2821 ggccggcccc cgcgcagcag cggcacggcc agggcgttgg tcacgtgctg gtcgctggcg
     2881 cgcgggatgg cgccggcgaa atgctggtag ctgttctgca cctggggccg gcccgtggtg
     2941 aaggccgtgc cggtgaggcc ctggccgggc gccacgggcg ggtaatcgta ggcggtgccg
     3001 ctcgacatgc gcgtcaccag tttgcccgaa tcctcgtccc agaggatcaa cgcgccgccg
     3061 ctggccccga ctagatcgac cgattgcccg atcagcatgc gcagcactgt ctccagatcg
     3121 agtgtggcct ccagcgaggt ggccagggcc tgcatcgttt cgagcgcccc ggcgcgctgt
     3181 tccgcggcgc tcagagcgcg ggtatgcacc agcaactgcg cggccaggcg ggccaggcgc
     3241 cggatcagtg cctgctccag cggggtatag tggtgcgggg cggggccggc cagatagatg
     3301 gcgcccagga agtcgccgtc gatctggagc ggcgccgcca gcatcgtgtt cgcgccaatc
     3361 ccgcgcgcca ggatacgccc gaccagcgtc tggctggtgg ccgccgcgta atcttcgacg
     3421 atgaacaact caccccggcg caacgtctgg tatagccggc tttccaggga gaactccagc
     3481 accgggggta acgccgccag cggcacgccc tccgttgcca cgaggcgcag ttcgtccgta
     3541 tgtggatcgc gcaacaccac gccgctccac ggcaggccca ccagcgcgac ggctgcggcg
     3601 gtgatacggg ccagcaggtc gtcggggcgg gtcgagcgcg ccaccgcctc gctgaaagcc
     3661 agcagcgtat ccaccgcgcc ggccggcaga tccggcaccg ccgccggctc aggcgcgggc
     3721 gcgccggtgc gacgaccggc ggagtcagga ctcatataga tctccgggac agggcggcca
     3781 gggatgcgga gcaatctcgg gcaaggaaag agggacggcg ccgcgcgcgg cgcggcgccg
     3841 gcagcggtta accgagttgg gccatcgcct tggcaagcgc ct
//
LOCUS       DASUYC010000209         4748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_663645,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000209 DASUYC010000000
VERSION     DASUYC010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4748
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663645"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="VF276_03350"
     CDS             complement(<1..739)
                     /locus_tag="VF276_03350"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEX6605932.1"
                     /translation="MDRNQLHNPFLDRLAERFATAGHRLYLVGGVVRDVLAEHPPGDM
                     DLATDAPVETVKRLAAKARPQTIYAVGEKFGTVGVIAGEHSYEITTFRTPPDGGPPPV
                     PPPDDPTPPDLYADLAYRDFTMNALAYDLATGTLYDPFEGRADLAARIVRGVGDPAAR
                     FAEDPLRMLRAVRLALQIGGQLDPETRLAATAQAPLLASVAWERKAAEMNRILLAPRP
                     SPGIRLLDELGLLAQVIPEITPMHGMAQ"
     gene            complement(835..1035)
                     /locus_tag="VF276_03355"
     CDS             complement(835..1035)
                     /locus_tag="VF276_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605933.1"
                     /translation="MHEDWIKREVRSFTSAAASLLNKNAPKVELPIAPHTAEGTPPPE
                     DPPATPPRSRRTARRRRKQAKR"
     gene            complement(1060..1818)
                     /locus_tag="VF276_03360"
     CDS             complement(1060..1818)
                     /locus_tag="VF276_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEX6605934.1"
                     /translation="MTAPPQTLAAALAAIRERIARAAERAGRDPATVTLIAVTKTQPL
                     TRIAEAAALGVRDFGENRVQEALAKFAPPDGSAEGAHSPEGADLIPRPALTLHLIGTL
                     QRNKARRAAAFFDSIQSVDRRDLLADLERHRASDAPDRPPLPVLIEVNISGEAAKTGA
                     APADVPALADALRACPHLAPRGLMTIAAPGLDEAALRRQFAAVRELRDQLQAAHPDVA
                     WSELSMGMSDDFEAAIQEGATMVRLGRALFGERR"
     gene            complement(1823..2731)
                     /gene="pgeF"
                     /locus_tag="VF276_03365"
     CDS             complement(1823..2731)
                     /gene="pgeF"
                     /locus_tag="VF276_03365"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEX6605935.1"
                     /translation="MALSATDASISPPVPPIYLLAGFAAYPDLVQGCTTRCRPAGPWG
                     PAGDWNLKRRGADPARADANWAAFAATLGIPATRMVAPEQIHGTEVGAVTEADAGLGS
                     LAGQRGIAGADGLITRTPGLLLLTGSADCVPVFVYDPVTPAVGLAHAGWKGTAGRIAE
                     TTVQAMTRAYGSDPTRLVAVMGPSIGPCCYDVGGEVVEAIRDAYPGADDEWQGEPPLL
                     VWSRRRPAWKDQQDLPRTEPESDEKPFLDLWQANRRALLLAGLAPENIAIDGICTAET
                     TELFYSHRAEQGRAGRFVAFLGLPEA"
     gene            complement(2700..3098)
                     /locus_tag="VF276_03370"
     CDS             complement(2700..3098)
                     /locus_tag="VF276_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605936.1"
                     /translation="MNLKLRRSARTPHSEEISIFDADVKDENEEPMNIGKLDVHYVED
                     QIVGTLLVWAEYAGRLNADQRTDLIDEILDEVSEPLGVSAEYGIEVYYPRLEQHEFVS
                     NYDEFEDEDDLIDEFEEEPADGTERDGRIH"
     gene            complement(3113..>4748)
                     /locus_tag="VF276_03375"
     CDS             complement(3113..>4748)
                     /locus_tag="VF276_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX6605937.1"
                     /translation="ESAVQAATAPAAAAPARAVRAAEAQTGGVWVLSGVVFVGGMVTM
                     GLEMTASQLLRPYFGSSLFIWANLIGLVMIYLTVGYYLGGRLADRYPRTGVLYGMVAA
                     AGIAAAVIPLLARPILSWSLEAFAEVSVSLFYGSLAGVIALFAIPMILLGCISPFAIR
                     LRMNQVDSAGETAGSIYALSTLGSILGTLVPVFLLLPTIGSARTLYLGAGALLIVAAL
                     GMLVTRHAGSRVVAMALLAVVALFLLPPSGLIKPPPYGTLLAEQESVYNYIQVVQQKN
                     GDIHLVLNEGHAIHSVYRADQSQPLTGGPWDYWLVAPYVNADMRPDDVHNMLMLGSAA
                     GTAAGLFTDVYGPKPVDNVEIDPAVVAMGRKYFHLDEPNVNNIIQDARAYLRTTDKKY
                     SIIGIDAYRQPYIPFHLTTREFFEEVRDKLEPNGVVMINAGRTATDYRLVDAIAATMR
                     TVYPHVYTIDVAGTGNTMVIGSMQDDGLAHFQANAAAIHQPLLQQVFAVSLDPNIGKM
                     REFTPADAAGHPVFTDDRAPVEEVIDQLILSYVTGQ"
BASE COUNT          680 a         1575 c         1732 g          761 t
ORIGIN      
        1 cctgggccat gccgtgcatc ggcgtgattt cggggatcac ctgggccagc aggcccaact
       61 cgtcgagcag gcggatgccg ggcgagggcc ggggcgcgag caggatgcgg ttcatctcgg
      121 cggccttgcg ctcccaggcc acgctcgcca gcaggggcgc ctgtgcggtg gcggccaggc
      181 gcgtctccgg gtccagttgc ccgccgattt gcagcgccag ccgcacggcg cgcagcattc
      241 gcagggggtc ctcggcgaag cgggccgccg ggtcgcccac gccgcgcacg atgcgcgcgg
      301 ccaggtcggc gcggccctcg aagggatcgt agagcgtacc cgtcgccagg tcgtaggcca
      361 gcgcgttcat cgtgaagtcg cggtaggcca ggtcggcgta gaggtcgggt ggcgtcgggt
      421 cgtccggcgg cggcacgggc ggcggcccac cgtccggcgg cgtgcggaag gtggtgatct
      481 cgtagctgtg ctcgcccgcg atgacgccga cggtgccgaa cttttcgccc acggcgtaga
      541 tcgtctgggg gcgggccttg gccgccagcc gcttaacggt ctcgaccggg gcatcggtcg
      601 ccaggtccat gtcgccgggc gggtgttccg ccagcacgtc gcggacgacg ccgccgacca
      661 ggtacagccg gtgccccgcc gtggcaaacc gctcggcgag gcgatccaga aatgggttgt
      721 gcagttgatt tcggtccatg acgagtggtc agtagtcggt ggtcagtggt cagtaatcac
      781 tggtatggag catcgccatc cctgagtagg gggcatctga cagtcggtat ttcgttagcg
      841 tttggcctgc ttgcgccggc ggcgggccgt gcggcgggag cgcggcggcg tagcgggcgg
      901 gtcttccggc ggcggggtgc cctccgcggt gtgcggggca atgggcaact cgactttcgg
      961 tgcgttcttg ttcagcaagg acgccgccgc ggaggtaaag ctgcgcactt cccgtttaat
     1021 ccagtcttcg tgcatggtcc tgttccttta cataatatct cagcgccgtt cgccgaacag
     1081 cgcgcggccc aggcgcacca tcgtcgcgcc ttcctggatg gcggcctcga agtcgtcgct
     1141 catgcccatg cttagctcag accatgccac gtccgggtgt gccgcctgga gttggtcgcg
     1201 gagctcgcgc acggcggcga actggcggcg cagcgccgct tcgtccaggc cgggcgcggc
     1261 gatggtcatc agcccgcgcg gggccaggtg cgggcaggcg cgcagcgcat cggccagggc
     1321 cggcacatcg gcgggcgccg cgcccgtctt ggccgcctcg ccgctgatgt tgacctcgat
     1381 cagcaccggc aggggcgggc ggtccggcgc gtcgctggcc cggtggcgct ccaggtcggc
     1441 cagcaggtcg cgccggtcca ccgactggat cgaatcgaaa aaagcagcgg cgcggcgtgc
     1501 tttgttgcgc tgcaatgtgc cgatgagatg cagggtcagg gcggggcgcg ggatgagatc
     1561 cgcgccctcg ggcgagtgtg ccccctcggc agagccgtct ggcggcgcga acttcgccag
     1621 cgcctcctgc acgcggttct cgccgaagtc gcgcacccct agcgcggcgg cctcggcgat
     1681 gcgcgtgagc ggctgggtct tggtcacggc gatcagcgtc accgtggccg ggtcgcgccc
     1741 ggcgcgctcg gcggcgcggg cgatgcgctc gcggatagcg gccagggccg cggccagggt
     1801 ctggggcggg gccgtcaccg ggctatgcct ccggcaagcc gaggaaggcc acgaagcgcc
     1861 cggcccggcc ttgctcggcg cggtgcgagt agaacaactc ggtcgtctcg gcggtgcaga
     1921 tgccgtcgat ggcgatgttc tccggggcca gcccggccag cagcagggcg cgccggttgg
     1981 cctgccagag gtccaggaac ggcttctcgt cggactccgg ctcggtgcgc ggcagatcct
     2041 gctggtcctt ccaggcgggg cggcggcggc tccagaccag cagcggcggc tcgccctgcc
     2101 actcgtcgtc ggcgccggga taggcgtcgc ggatggcctc gaccacctcg ccgcccacgt
     2161 cgtagcagca cggcccgatg ctggggccca tgaccgcgac caggcgggtc gggtcgctgc
     2221 cgtaggcgcg ggtcatggcc tgtaccgtgg tctcggcgat gcgccccgcc gtgcccttcc
     2281 accccgcgtg ggccagcccc acggcgggcg tcaccgggtc gtagacgaag acgggcacgc
     2341 agtcggcgga gccggtgagc agcagcagcc ccggcgtgcg cgtgatcagc ccgtcggcgc
     2401 ccgcgatgcc gcgctgcccg gccaggctgc ccaggcccgc gtcagcctcc gtcaccgcgc
     2461 cgacctccgt gccgtggatc tgctccggcg ccaccatgcg cgtggccggg atgcccagcg
     2521 tggcggcaaa ggcggcccag ttggcgtcgg cgcgggccgg gtcggcgccg cgccgtttca
     2581 ggttccagtc gcccgccggt ccccaaggcc ccgccgggcg gcaacgcgtg gtgcagccct
     2641 gcaccagatc gggatacgcg gcgaacccgg ccaggagata gatgggcggg acaggcgggc
     2701 taatggatgc gtccgtcgcg ctcagtgcca tcggccggtt cttcctcaaa ctcgtcgatc
     2761 aggtcgtcct cgtcctcgaa ctcgtcgtag ttggagacga actcgtgctg ctccaggcgc
     2821 gggtagtaga cctcgatccc gtactctgcc gacacgccca gcggctcact gacctcgtcg
     2881 aggatctcgt cgatgaggtc ggtgcgctgg tcggcgttca ggcgcccggc gtattcggcc
     2941 cagaccagca gcgtgccgac gatctggtct tccacataat gcacgtcgag cttgccgatg
     3001 ttcatcggct cctcgttctc gtccttgaca tcggcgtcga agatcgagat ctcctcggag
     3061 tggggcgtgc gggcactgcg gcgcagcttc aggttcatgg ctctggctcc ttttactgac
     3121 ccgtgacgta gctcaagatg agctgatcga tcacttcttc gacgggggca cggtcgtcgg
     3181 tgaagacggg gtgcccggcg gcatcggcgg gcgtgaactc acgcatcttc ccaatgttcg
     3241 gatccaacga gaccgcgaag acctgttgga gcagtggctg gtggatggcg gcggcgttgg
     3301 cctggaagtg ggccagcccg tcgtcctgca tgctgccgat gaccatcgtg ttgccggtgc
     3361 ccgccacgtc gatggtataa acgtgcggat agaccgtgcg catcgtggcg gcgatggcat
     3421 cgactagccg atagtcggtg gcggtgcgtc cggcgttgat catgaccact ccgttcggct
     3481 ccagcttgtc gcgcacttcc tcgaagaact cgcgcgtggt caggtggaac gggatgtacg
     3541 gctggcggta ggcgtcgatg ccgatgatgc tgtatttctt gtcggtggtg cgcaggtagg
     3601 cccgcgcatc ctggatgatg ttgttgacgt tcggctcgtc caggtggaaa tacttgcggc
     3661 ccatcgcgac caccgcgggg tcgatctcga cgttgtcgac cggcttaggg ccgtatacgt
     3721 cggtgaacag ccccgccgct gtgcccgccg ccgagccgag catgagcatg ttatgcacat
     3781 cgtcgggccg catatcggcg ttgacgtagg gcgccaccag ccagtagtcc caggggccgc
     3841 cggtcagcgg ctgcgactgg tcggcgcgat agacgctgtg gatggcgtgg ccctcgttga
     3901 gcaccagatg aatatcgccg ttcttttgct ggacgacctg gatgtagtta tagaccgact
     3961 cctgctcggc cagcagcgta ccgtagggcg gcggcttgat caggccgctc ggcggcagca
     4021 ggaacagggc gaccaccgcc agcagcgcca tggccaccac ccgcgacccg gcgtgccgcg
     4081 ttaccagcat gccaagcgcg gcgacgatca gcagcgctcc cgccccaaga tacagcgtgc
     4141 gcgcgctgcc gatggtgggc agcagcaaga agaccgggac cagcgtgccc aggatgctgc
     4201 ccagcgtgga tagggcgtag atcgaccccg ccgtctcgcc cgccgagtcc acctggttca
     4261 tgcgcaggcg gatggcgaac ggcgagatgc agccgagcag gatcatcggg atggcgaaca
     4321 gggcgatgac cccggccagc gagccgtaga acaggctgac cgagacctcg gcgaaggctt
     4381 cgagcgacca ggagaggatg ggccgggcca gcagcggaat cacggcggcg gcgatgcccg
     4441 cggcggccac catgccgtag agcacgccgg tgcgcgggta gcggtcggcc aggcgcccgc
     4501 ccaggtagta gcccacggtc aggtagatca tcaccaggcc gatcaggttg gcccagataa
     4561 acagcgagct accgaagtag ggccgcagca actgcgaggc ggtcatttcc agccccatcg
     4621 tcaccatgcc gcccacgaag accacgccgg acagcaccca cacgccgccg gtctgcgctt
     4681 ccgccgcgcg cacggcccgc gcgggggcgg cggcagcggg tgccgtggcg gcctggacgg
     4741 cgctctca
//
LOCUS       DASUYC010000210         2673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_108773,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000210 DASUYC010000000
VERSION     DASUYC010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108773"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VF276_03380"
     CDS             <1..621
                     /locus_tag="VF276_03380"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6605938.1"
                     /translation="VPVVESLRQAGHYMQSTRVVGLLVLLSALVGFFGRSYIQLTPAF
                     ARDLLHVDARGLGLLYAAPGLGALLGAGVLAGRRDMRVEQRILIGSVLAFAGLLIAYS
                     FDPWFPVALVLLTGTGISSQVAATMISTTLQLQTPGRLRGRILSFYAITIIGLASLGA
                     LATGILAEITGPGIAVALGAVIMGGAALLIAPHIHGMDALNADAAR"
     gene            complement(694..1560)
                     /locus_tag="VF276_03385"
     CDS             complement(694..1560)
                     /locus_tag="VF276_03385"
                     /inference="COORDINATES: protein motif:HMM:NF014754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEX6605939.1"
                     /translation="MEQRAGAAQVPVAKVLEMIAATLYQERGRLNGLSSFGGDARHGD
                     RMARAFGDAADAVVAAGIGNAGKELQLAGQILADPAYGRASRYFGQGLSYAAPHFMGQ
                     AGLSLADLGTLLTDLLEGVRADNPAQPGMGTMIDVLIPATMAYTGAVEQGLTYNQAIQ
                     GLLGAAMSGARSTANMPQPYTRTQGGGTGSAGQIDVGAAFAQTVLTGLVKALLGDKVP
                     PTPAGQNSDNFLLNLIQQGIDLGNIVAAPRQSEVESLLGATGRAGRAVRPLDATDPGY
                     GKGPAMVGETKR"
     gene            complement(1585..>2673)
                     /locus_tag="VF276_03390"
     CDS             complement(1585..>2673)
                     /locus_tag="VF276_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX6605940.1"
                     /translation="QLDLEMSFVEREDILQLIEGMLTEMVPAVSEKRLMTTPFPRLKY
                     ADVLERYGSDKPDIRFGMEIHDLSDIVRGSEFAVFRDALQAGGVVRGIAAPGCAGYTR
                     RELDDLTEQAKRAGARGLVWVALENRTPDGWQIRSSAAKYLQPAEVDGMADALEAKAG
                     DLLLLVADKRETAGAVLGKLRVEMARRLNLLDDDVLAFAWVLDPPLLEWNTDENRWDA
                     VHHPFTMPMPEDIPLLDSDPGAVRATAYDIVCNGFELSTGSIRIHERALQAKVFGLMG
                     YSDEEATFRFGHLLEAFEYGAPPHGGIAPGIDRIVMLLAGKDNIRDVIAFPKTQSATD
                     LMTDAPSPVPEQALRELHIRVREKDPRS"
BASE COUNT          377 a          902 c          938 g          456 t
ORIGIN      
        1 gtgccggtgg tcgaaagcct gcgccaggcc gggcactata tgcaatcgac gcgggtggtg
       61 ggtttgctcg tgctgctgtc ggcgctcgtc gggttcttcg ggcggtccta catccaactg
      121 acgcccgcct ttgcgcgcga cctgctgcat gtggacgcgc gggggctggg gttgctgtac
      181 gcggcgccgg ggctgggcgc gttgctgggc gcgggcgtgc tggccgggcg gcgcgatatg
      241 cgcgtggaac agcgcatcct gatcggatcg gtgctggcct tcgccgggct gctgatcgcc
      301 tacagcttcg atccctggtt cccggtggcg ctggtgctgc tcaccggcac cggcatcagc
      361 agccaggtgg cggcgacgat gatcagcacc acgcttcagc tccagacgcc gggccggctg
      421 cgcgggcgca tcctctcgtt ctacgcgatc accatcatcg gcctggcctc gctgggcgcg
      481 ctcgccaccg gcatcctggc cgagatcacc ggcccaggga tcgccgtcgc gctcggcgcg
      541 gtgatcatgg gcggggccgc gttgctgatc gcgccgcaca tccacggcat ggacgccctg
      601 aacgccgacg ccgcgcgctg agccgggccg gaaagcgcaa cagggctggc cgccggacgc
      661 cccgccctgt tgtgttaggc cgatcccgga ttgttagcgc ttcgtttcac cgaccattgc
      721 cggccctttg ccatagccgg gatcagtggc gtcgagcggt cggacggcgc gcccggcgcg
      781 gcccgtggcc cccagcaggc tctcgacctc gctctggcgg ggcgcggcga cgatattgcc
      841 caggtcgatg ccctgctgga tcaggttgag caagaaattg tcgctgttct gcccggcggg
      901 ggttggcggc actttgtcgc ccagcagcgc cttgaccagg ccagtcagca cggtctgcgc
      961 gaaggcggcg cccacgtcga tctggcccgc cgagccggtg ccgccgccct gcgtgcgcgt
     1021 gtagggttgg ggcatattgg ccgtgctgcg tgcgccgctc atggccgcgc cgagcaggcc
     1081 ctggatggcc tggttgtagg tcagcccctg ctcgaccgcg ccggtgtagg ccatcgtggc
     1141 ggggatcagc acatcgatca tcgtgcccat gcccggctgg gccgggttgt cggcgcgcac
     1201 gccttcgagc aggtcggtga gcagcgtccc gaggtcggcc agactgaggc cggcttggcc
     1261 cataaagtgc ggcgccgcgt agctcagacc ctggccgaaa tagcgcgacg cccggccata
     1321 ggcgggatcg gcgaggatct gccctgccag ttgcagttcc ttgccggcat taccgatgcc
     1381 cgcggcgacg acggcatcgg cggcatcgcc aaacgcgcgg gccatgcgat caccgtgtct
     1441 ggcgtcgccg ccgaaactcg acaggccgtt caaccggccc cgctcctggt agagggtggc
     1501 cgcgatcatc tccaggacct tggcaacggg gacctgggcc gcgccggcgc gctgttccat
     1561 aaaacgtgcc tcctgccttt cgcgttagga tcgggggtct ttctcgcgca cccggatgtg
     1621 cagttcccgc agcgcctgtt cgggcacggg agacggcgca tcggtcatca aatcggtggc
     1681 cgattgcgtc ttggggaacg caataacgtc acgaatgtta tccttgcccg ccagcagcat
     1741 gacgatgcgg tcgatgccgg gggcgatgcc gccatgaggc ggcgcgccat actcgaaggc
     1801 ttcgagcagg tgcccgaagc ggaaggtggc ctcctcgtcc gagtagccca tcaggccgaa
     1861 caccttggcc tgcaatgccc gctcatggat acggatcgag ccggtcgaaa gctcgaagcc
     1921 gttgcagacg atatcgtagg ccgtggcgcg caccgcgccg ggatcgctgt ccagcagcgg
     1981 gatgtcctcg ggcatgggca tggtgaaggg gtgatgcacg gcgtcccagc ggttttcgtc
     2041 ggtgttccac tccagcagcg gcgggtcgag cacccaggcg aaggccagca cgtcgtcgtc
     2101 aagcaggtta aggcggcggg ccatctcgac gcgcagcttg cccagcacgg ccccggcggt
     2161 ctcgcgcttg tcggccacga gcagcagcag atcgcccgct ttggcctcca gcgcatcggc
     2221 catgccgtcc acctcggcgg gttgcagata cttcgccgcc gacgagcgga tctgccagcc
     2281 gtcgggggtg cgattctcca gcgccaccca gaccaggccg cgggcgcccg cccgtttcgc
     2341 ctgctcggtc aggtcgtcga gttcgcggcg cgtgtagccc gcgcagccgg gggcggcaat
     2401 gccgcgcacg acgccgcccg cctgcaacgc atcgcggaac acggcgaact cgctgccgcg
     2461 cacgatgtcg ctcaggtcgt ggatctccat gccgaagcgg atgtcgggct tatcggagcc
     2521 gtagcgctcc agcacgtcgg cgtatttcag gcgcgggaac ggggtagtca tgaggcgctt
     2581 ctcgctgacg gcgggcacca tctcggtcag catgccctcg atcagttgca ggatgtcctc
     2641 gcgctcgaca aagctcatct ccaggtcaag ctg
//
LOCUS       DASUYC010000211         2189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_87168,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000211 DASUYC010000000
VERSION     DASUYC010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87168"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VF276_03395"
     CDS             <1..681
                     /locus_tag="VF276_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605941.1"
                     /translation="LEDLSVDERNRFITTIAQLEARSAYMIRSYIARHLTASAILPLP
                     MYEVRALVNDAVNRGLFQRGTRPFRHPATGEMQSLETLLLDRGHPWVAEALAGQAAPS
                     DEDTADSDQDLPREAGDAGDWDAEDHDHWTDDAPAASDGHSHAEPAGSDEPEDAVAAP
                     ARPRPAIEAAEGEDELDARPASPAALNGTAGAHAGGDHGGYRRPAYEPAGGEHGPRPA
                     YDEDEDQY"
     gene            complement(739..>2189)
                     /locus_tag="VF276_03400"
     CDS             complement(739..>2189)
                     /locus_tag="VF276_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605942.1"
                     /translation="RKQVIVKEPAPAAPQVRRALRPPAPEKAPAPPAAAAPDDSRPLP
                     YDWVLLVGLAAIVAIIDPIVIDMQHHIEVSLIILAAGVGLGWWGLTRARRAAVWTGAA
                     ILWAGIVAGIATQIAPLGVAAGTIWAPRGALLVAALLGWLLVRELPRALWRGVLALAV
                     PTLVVLAWLWWTAPAFARAAQVYWLATDSRGNVYASDDTDGVVWRFDSDGHVLGKLWP
                     RWAKPPGTPGPGYGPAGVSSTLLEPFARQTAVPIGPIVRHFSFCGIAIGPDDGLYTVD
                     LDTLQILDFDRDGQLRHSWPLPAGYVPAQGCIAADAQHLYLADLRSSIHVYDFTGHEQ
                     TQWPVTQTPHGLAPTPDGRLLVLHERSVSFLQPPTGQVVGEWTLPPSSGVLLAAYQTV
                     TVRHNGEVLITDYNANMIVRYAADGTPLPAWGGPGTQPGQFAAPAGITEGPQGQVYAA
                     DSTHHAIQRFQGDGQVDRVWSASADDPIEAGE"
BASE COUNT          326 a          765 c          824 g          274 t
ORIGIN      
        1 ctggaagacc tctcggtgga cgaacgcaac cgctttatta cgacgattgc ccagttggaa
       61 gcgcgcagcg cctacatgat ccgctcgtac atcgcccgcc acctgacggc gtcggccatc
      121 ctgccgctgc cgatgtacga agtgcgcgcg ctggtgaacg acgcggtgaa ccgcggcctg
      181 ttccagcgcg gcacgcggcc cttccgccac ccggccaccg gcgagatgca gagcctggaa
      241 acgctgctgc tggatcgcgg ccacccctgg gtggccgagg ccctggccgg gcaggccgcg
      301 cccagcgacg aggataccgc cgacagcgat caggatctgc cgcgcgaagc cggcgatgcg
      361 ggtgactggg acgccgagga tcatgaccac tggacggacg acgccccggc ggctagcgac
      421 ggccacagcc acgccgagcc tgcgggatca gatgaaccgg aggatgcggt ggcggccccg
      481 gcccgcccgc ggcccgcgat agaagccgcg gaaggggaag acgaacttga tgcgcgcccg
      541 gccagccccg ccgccctgaa cggcaccgcc ggcgcacacg cgggcggcga ccacggcggc
      601 taccggcggc ccgcctatga accggcgggc ggcgagcacg gcccgcgccc cgcctatgac
      661 gaggacgagg accagtatta gcgcctgatc gacggatctg cgcgacggcc ccggcgggat
      721 tccgccgggg ccgtgttgtt attcgcccgc ttcgatggga tcgtcggcgg aggcgctcca
      781 gacccggtcc acctggccgt cgccctggaa gcgctggatc gcgtggtggg tggagtcggc
      841 ggcgtagacc tggccctgcg gcccttcggt gatgccggcg ggcgccgcga actgccccgg
      901 ctgggtgccc ggcccgcccc aggccggcag cggcgtgccg tcggcggcgt agcgcacaat
      961 catgttggcg ttgtagtcgg tgatgagcac ttcgccgtta tggcgcaccg tgacggtctg
     1021 gtaagcggcc agcagcacgc cggacgatgg cggcagcgtc cattcgccca ccacctgccc
     1081 ggtgggcggc tggaggaacg agacgctgcg ctcgtgcagc accagcaagc ggccgtcggg
     1141 cgtcggggcc aggccgtgcg gcgtttgcgt caccggccat tgcgtttgct cgtggcccgt
     1201 gaaatcgtag acatgaatgc tgctacgcag gtcggccagg tagaggtgct gggcgtcggc
     1261 ggcgatgcag ccctgcgccg ggacatagcc cgcgggcagc ggccaggagt ggcgcaactg
     1321 gccgtcgcgg tcgaagtcca gaatctgcag tgtgtccagg tcaacggtgt aaaggccatc
     1381 gtccgggccg atggcgatgc cgcagaagga gaagtggcgc acgatggggc cgatgggcac
     1441 ggcggtctgg cgggcgaacg gctccagcag ggtggaactg acgcccgccg ggccgtagcc
     1501 ggggccgggg gtgccgggcg gtttcgccca gcgcggccag agtttgccca gcacgtgccc
     1561 gtcgctgtcg aagcgccaga ccacgccgtc ggtgtcgtcg ctggcgtata cgttgccgcg
     1621 cgaatccgtg gccagccagt agacctgggc ggcgcgggcg aacgcgggcg cggtccacca
     1681 cagccaggcc agcacgacca gggtcggcac ggccagggcc agcacgccgc gccagagcgc
     1741 gcggggtagc tcgcgcacca gcagccagcc gagcagggcc gcgaccagta gcgcgccgcg
     1801 cggcgcccag atcgtgcccg ccgccacgcc cagcggcgcg atctgcgtgg cgatcccggc
     1861 gacgatcccc gcccagagga tggccgcccc ggtccacacg gcggcccggc gcgcgcgcgt
     1921 cagcccccac caccccaacc ccacccccgc ggccaggatg atcaggctca cttcgatgtg
     1981 gtgttgcatg tcgatgacga tggggtcgat gatcgcgacg atggcggcga ggccgacgag
     2041 cagcacccag tcgtagggca gcgggcgcga gtcgtcgggc gcggcggccg ccgggggcgc
     2101 cggcgccttt tcgggggcgg gcgggcgcag cgcgcgccgg acctggggcg ccgccggggc
     2161 cggctccttc actatgacct gtttgcgac
//
LOCUS       DASUYC010000212         4849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_581247,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000212 DASUYC010000000
VERSION     DASUYC010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4849
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581247"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            336..1418
                     /locus_tag="VF276_03405"
     CDS             336..1418
                     /locus_tag="VF276_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsO family NAD(P)H-dependent flavin-containing
                     monooxygenase"
                     /protein_id="HEX6605943.1"
                     /translation="MQSDPARRDYDVVVIGGGQAGLATGYYLRRSGLSFVILDGEPAP
                     GGAWQHTWDSLALFSPAQWSSLPGVFMPGGTHTYPDRDAVLAYLAAYEARYQLPVVRP
                     VQVQTVDRAEGGLVVTSDAGMWRARAMVSATGSWRHPYIPPYPGRDQFQGTQIHSAHY
                     RNAAGLAGQQVVVVGARNSGAQILAEVSQVAATTWVTREPPRFLPDDVDGRVLFDRAT
                     AYYQAQQAGGGSLAAAAQGALGQIVMVPPVRAARARGVLHSVRPFTRLTAHGAVWPDG
                     RETPVDAIIWCTGFFPALAHLAPLGILTPEGQVATQGTCALAEPRLWLVGYGDWTGFA
                     SATLIGVGRSARQTVEEIKATFQPPC"
     gene            complement(1544..1843)
                     /locus_tag="VF276_03410"
     CDS             complement(1544..1843)
                     /locus_tag="VF276_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605944.1"
                     /translation="MIPTDKLRQFARKAAELRDQQLAAGNDDQLTARAVIGALDAPPD
                     EIDYFVHEVPTLVPVLARRGLVVIEHEPAPEHPDATADDYAFRLTAQGIFWARSG"
     gene            complement(2164..3588)
                     /locus_tag="VF276_03415"
     CDS             complement(2164..3588)
                     /locus_tag="VF276_03415"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6605945.1"
                     /translation="MTTMNEPVAQTAGLRAVLHNGPFLRLWLAQALSQTAQQMISYVL
                     LVQVAAISQSSAAVSGIMIAFALPGILFSALAGIYVDRRAKRTVLVATNAGRTLALLG
                     YILLTSPVLRFDPLLVLYITALVFASVSQFFMPAEGAAIPLLVGRDHLLPANSLFNLT
                     FTASQLLGFLLLGPLCVKVLVHGTDFGAFYAILAGLFLVCTLLTAMLPRGEQVKPVAE
                     IDGVGSAVQSVLADLREGWQYIRNDAAIYSAIIQSSVATAVVLMLGVIGPRFLAEEVG
                     IPASDLYLVLAPGGIGLGIGVALVGRFSTEANRMRMISLATFAAGLGLLAFAALSPAA
                     QLLAGMVGGAAGTHAWVLGAMMVLVLILGALNSFVTVPAQTVLQERARDDVRARVLSA
                     FNTVSNIGVTAATFFAGALADAVGVATTVGLIGLGVAGVASWGIRQATAPARRRVVAP
                     LPARSGKLSTGPLTPLPSGSRKAA"
     gene            complement(4073..4501)
                     /locus_tag="VF276_03420"
     CDS             complement(4073..4501)
                     /locus_tag="VF276_03420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6605946.1"
                     /translation="MKHILLVDDDPGLRQLLLVTLAAPEFLIRQVSSGKMALRVAREF
                     QPDLIILDLYLTPNQAEGLEVCRQLKTDPLTARSYILMLTGANRPEDRAAAAAAGADF
                     YFTKPFSPRALLAHIYTVLGLEPGGPPAVVRAPQRHIPPS"
BASE COUNT          833 a         1706 c         1590 g          720 t
ORIGIN      
        1 gagccgggtc cgccccggaa atcggttgcg aattatagtc ttgcatgata ctctcccctc
       61 atcgctgccc ccacggggca ctacaacaaa acggtgcggc cctcgcggac cggctgcgtt
      121 ggcgtctttc ggttggaacc ccggcggtcg ccccttgcat gggccgccgg ggttcgtaca
      181 gttccattat cctgcgccca ccgcagaaca accgcagagc tagagcagaa tgaggtaaga
      241 gtttggtcct gggcaccggc acaccggctg gtgtatcatg gcggtgcaca gagccatacg
      301 ggcaaccagg gccaacaagg gggaaacaac gcgccatgca gagcgatccg gcccggcgcg
      361 actatgatgt cgtcgtcatc ggcggcgggc aggcgggcct cgccaccggc tattacttgc
      421 gccgtagcgg cctgtcgttc gtcattctcg atggcgaacc ggcccccggc ggcgcctggc
      481 aacacacctg ggactcgctg gcgctcttct ccccggcgca gtggagttcg ttaccggggg
      541 tcttcatgcc gggcggcacg cacacctatc cggaccgcga tgccgtgctt gcctatctgg
      601 ccgcgtatga ggcccgctac cagttgcccg tcgtccgccc ggtgcaggtg caaaccgttg
      661 accgcgccga gggcgggctg gtcgtgacga gcgatgccgg gatgtggcgg gcgcgcgcca
      721 tggtcagcgc caccggctcc tggcgccacc cctacatccc gccctatccg gggcgtgacc
      781 agttccaggg cacgcagatc cactctgctc actatcgcaa cgcggcgggc ctcgccgggc
      841 agcaggtggt ggtggtcggg gcgcgcaatt cgggcgcgca gatcctggcc gaggtctccc
      901 aggtggcggc taccacctgg gtcacgcgcg aaccgccgcg cttcctgccc gacgacgtgg
      961 acgggcgcgt gctgttcgac cgcgccacgg cctactacca ggcgcagcaa gccggcggcg
     1021 gatcgctggc cgcggcggcc cagggcgcac tcggccagat cgtcatggtg ccgccggtgc
     1081 gcgcggcgcg cgcccgcggc gtgctgcaca gcgtccgccc gttcacccgc ctgaccgcgc
     1141 acggcgccgt ctggcccgac ggccgcgaaa cgccggtgga tgcgatcatc tggtgtaccg
     1201 gcttcttccc cgccctggcc caccttgcgc cgctcggcat cctcacgccg gaggggcagg
     1261 tcgccacgca gggcacgtgc gccctggccg agccgcgcct ctggctcgtc ggctacggcg
     1321 actggaccgg cttcgcctcg gccaccctca tcggcgtggg ccgcagcgcc cgccagaccg
     1381 tcgaggagat caaggccacc ttccagccgc cgtgctgaca tccggttaac cggtggtctt
     1441 tctcaccgcg aagacgcgaa ggacgcgaag aacaaaacta acataaataa ccttcgcgtc
     1501 cttcgcgtct tcgcggtgaa ttaacaaacg gatatccagc cgatcacccg ctacgcgccc
     1561 agaagatgcc ttgcgccgtc agccggaagg cgtagtcgtc cgccgtcgca tccgggtgct
     1621 ccggcgcggg ttcgtgctca atgaccacca ggccccggcg cgccagcacc ggcaccaggg
     1681 tgggtacctc gtggacaaaa tagtcgattt cgtccggggg cgcatccagg gcgccaatca
     1741 ccgcccgcgc cgtcaactga tcgtcattgc ccgccgcaag ttgctggtcg cgcagttccg
     1801 ccgccttccg cgcgaactgc cgcagcttat ctgtagggat cattgcctgc taccccagtg
     1861 catccgtgat ttatgaccag ggagtggccg ggtagcgaac gctacccggc acgggggacg
     1921 gcccatctac catccacacg cggcgacggc caacgcggcg ctgtggcccc aggttcagca
     1981 tacggcgccc ggcttgcagt cgcgtgaacg cgccccccgc gcctgccgac agttttatag
     2041 ccggtctgtg agcgcccatc cttaccgttt gctaaggatt gcgccggcca ccgggcacac
     2101 aaaacgggcg ccggataaac cggcgcccgc ctcgcgtatt tgcttgtagt ccggtgtgcc
     2161 ggctcaggcc gccttgcgcg aaccggacgg cagcggggtc aacgggccgg tgctcagctt
     2221 gccgctgcgg gccggcagcg gggccaccac ccgccgccgg gccggcgccg tcgcctgccg
     2281 gataccccag ctggccaccc cggccacgcc cagcccgatc aggcccaccg tggtcgccac
     2341 gccgaccgcg tcggccagcg ccccggcgaa gaaggtcgcc gccgtcacgc cgatgttcga
     2401 gacggtattg aaggcgctca gcacccgcgc ccgcacgtcg tcgcgcgccc gttcctgcag
     2461 caccgtctgg gccggcacgg ttacgaacga gttgagcgcg cccaggatca gcaccagcac
     2521 catcatcgcg cccagcaccc aggcgtgggt gcccgcggcg ccacccacca ttccggccag
     2581 caactgggcc gccgggctga gggccgcgaa ggccagcagc cccagccccg ccgcgaacgt
     2641 cgccaggctg atcatgcgca tccggttcgc ctcggtcgag aagcgcccga ccagcgccac
     2701 gccgatgccc agcccgatgc cgccgggggc cagcaccagg tacaggtccg atgccgggat
     2761 gcccacctct tcggccagga agcgcgggcc gatcacgccc agcatcagca ccaccgccgt
     2821 cgccaccgac gactggataa tggcgctgta gatcgcggcg tcgttgcgga tgtactgcca
     2881 gccttcgcgc aggtcggcca gcaccgactg gaccgcgctg ccgacgccgt cgatctccgc
     2941 gaccggcttc acctgctcac cgcgcggcaa catagccgtc agcagcgtgc agaccaggaa
     3001 cagcccggcc aggatcgcgt agaaggcccc gaaatcggta ccgtggacca gcaccttcac
     3061 acacagcggc cccagcagca ggaagccgag caactggctc gccgtgaagg tcaggttgaa
     3121 cagcgagttc gcgggcagca gatggtcgcg gcccaccagc agcgggatcg ccgcgccttc
     3181 cgccggcatg aagaactggc tcaccgaggc gaagaccagg gcggtgatat acagcaccag
     3241 cagcggatcg aaccgcagga cggggctggt cagcaggata tagccgagca aggccagcgt
     3301 gcgcccggcg ttggttgcca ccagcaccgt ccgcttggcc cgccggtcca cgtagatacc
     3361 ggccagcgcg ctgaacagga tgcccggcag cgcaaaagcg atcatgatgc cgctcaccgc
     3421 cgcgctcgac tggctgatcg ccgcgacctg caccagcagc acgtagctga tcatctgctg
     3481 cgcggtctgc gacagcgcct gggccagcca gagccgcagg aacggcccgt tgtgcaggac
     3541 ggcccgcagc ccggcagtct gagccacagg ttcgttcatc gtcgtcatgt aatcaagtct
     3601 cccgtccgta atacgcggcg ctcgttcaga aggtcacata gtaggggagg cgccacgcct
     3661 ggttcgcgtt gtatgtcacg cttagaatag cacggagtcg gggccgggca catgccgcca
     3721 ggggtgtaac acgggccgta tgattgccca tagtaacccg cgcggattct acgacttggg
     3781 aggtaggcaa aattgggcgg atgccgcacc cctcaccgcg tgctgatcgc gcagcggtgc
     3841 ggagcacatc gcgtaagcgg tgtgtagcac attgctgggt gctgagggct gagtacggtg
     3901 agtaacggcg gatgagtgca aaggggctag cgcgggaaag gagaggccga tttcaggggc
     3961 gcggcaatcc ggcgatgtag gggccggttg caaaccggct cgccgccgtc gcaaacaaca
     4021 gcaactcccc tccccgcagc gggggagagg ttggcccggc tacgcgcccg gcttatgagg
     4081 gcgggatgtg gcgctgcggc gcgcgcacca cggcgggcgg gccgcccggc tccaggccca
     4141 gcaccgtgta gatatgcgcc aacaaggcgc gcgggctaaa cggcttggtg aaatagaaat
     4201 cggcccccgc ggcagccgcc gccgcccggt cttcgggccg gttcgccccg gtgagcatga
     4261 ggatatagct gcgcgcggtg agcgggtcag tctttagctg ccggcacacc tccagccctt
     4321 ccgcctggtt cggcgtgagg tagaggtcca ggataatgag atccggttgg aattcgcggg
     4381 ctacgcgcaa cgccatcttg ccgctgctca cctggcggat caagaactcc ggtgcggcca
     4441 gcgtcaccaa caacagttgg cgcagcccag gatcgtcgtc gaccagcaaa atgtgcttca
     4501 tacagatccc acccgtgcgc cctgacgggc cggcgccctg gcggacgccg ggtaaattgg
     4561 gggcaagtac gcaggtccca acacgcatta tgttaccacc ggggtactga cgaaacactg
     4621 acaacaccgt gggccgccca tccacctgcg ccgcaaggcg tggaaccgcc gcgcggcgtg
     4681 agccgcccgg cgccgctttc agcatacccc aaacccggca ggcgcgctac agaccaagat
     4741 gcctatggcg gctgcgacct agtaccgaga atagaatccg cctttgggtg gagacgagtc
     4801 cctgcccgcg actccaggga gccggggcat ccgctgctat aatgacagc
//
LOCUS       DASUYC010000213         2267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_974744,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000213 DASUYC010000000
VERSION     DASUYC010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974744"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /gene="xseB"
                     /locus_tag="VF276_03425"
     CDS             complement(<1..274)
                     /gene="xseB"
                     /locus_tag="VF276_03425"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEX6605947.1"
                     /translation="MNAPSKLNLSYEEAYKRLEEAVAQLSAGGLSLEEALARFEQGMA
                     LANYCGTLLDQAELRVQQVGQLAPEEMEALIDGGTPAPAAQPAPEPP"
     gene            936..1607
                     /locus_tag="VF276_03430"
     CDS             936..1607
                     /locus_tag="VF276_03430"
                     /inference="COORDINATES: protein motif:HMM:NF017778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HEX6605948.1"
                     /translation="MVATVNRGLIDQTSLPGIIKAQPWQRTLARVTRELAQLVDLPAP
                     LAVREGRAVLAHLTQDREFLMAQIWPILGEVQAEGPYVARRYEAPGGAYSLQVFVWPA
                     GAQTQIHDHSCWGIFGPVAGTLREERYLRLDDGARADEAHLRADWRRLWTPDDGLSTL
                     LPYAGGVHRVSNRSLRPAISIHVYGPAGAIDGRDYDPLRDYVCDRLTDDTPGGLLLDD
                     LIAAC"
     gene            complement(1707..>2267)
                     /locus_tag="VF276_03435"
     CDS             complement(1707..>2267)
                     /locus_tag="VF276_03435"
                     /inference="COORDINATES: protein motif:HMM:NF014640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX6605949.1"
                     /translation="VARAVYASIVPVVTGVGHETDTTIVDYVADLRAPTPSAAAELVA
                     PDIRELIDDVEALRQELWDLMNGCLEQAAGDLAEQRAVLARHSPRAQVEQQRQRLDSL
                     AEAARLHLSHRLALQRAGLTTQQARLQALAPTAILTRGYVIARNRRTGHVAASIADIA
                     GGDILDLQFRDGTVPVRAGHPKPPQS"
BASE COUNT          375 a          764 c          770 g          358 t
ORIGIN      
        1 tcggcggctc gggcgccggc tgcgcggcgg gcgcgggcgt gccgccgtcg atcaacgcct
       61 ccatctcctc cggcgccagt tggcccacct gctgcacgcg caactcggcc tggtcgagca
      121 gggtgccgca ataattcgcc agcgccatgc cctgctcgaa gcgggccagc gcttcctcca
      181 ggctcaaccc acccgcgctg agctgggcca cagcctcttc caggcgcttg tacgcctctt
      241 catacgatag gttgagtttc gacggggcgt tcacacacgg ctccttacga gtactgatcg
      301 cgtcagcggt gcgtagcaca ttgctgagtg ctgagtacga acgaaagtca aacagcgcag
      361 cgctcggcga cgagtgaatc actgatagtc tgtgggtacg gtaggcccgg catggttcac
      421 ggggcaatac ggaattatac cacacgcagc ccggcagcgc gcaggctacg gcgcgcgcac
      481 aggtgggtgg caggatgtcg tggcccggcg cccgccccgc gcaatgcatc accccatagc
      541 gcggcgtcac tcgcatccgt cccgcgcccg gagcgggaca ggggcatcag gccggtccgc
      601 cgcccggcca gggccgcaag aggtctacgc aacgttacgt agtccttcgg cgctttgtat
      661 ccctccgcac gatacataat tcccccgatg tgcgggcctc tccgtcgtcg tatagtcaga
      721 tcgtgagcaa ttgcccggca aaatggcgca aagtgggtac acagtgaaca ttttttgatc
      781 tggtgagtga gcaaatgcca gggatacagt agagatgaga cgcaggagac accgggcacg
      841 gaccgcactc tccggcaggc cacgacggga ggccgctgca aaggcatcgc gctacaactc
      901 aacagcgagc gcgtagagta gggaggttat ttctcatggt cgcaacagta aatcggggcc
      961 ttatcgatca gacgagcctg ccgggcatca tcaaagcgca accgtggcaa cgcaccctgg
     1021 cccgcgtcac ccgcgaactg gcccaactgg tggatctgcc cgcgccgctg gccgtgcgcg
     1081 aaggccgcgc cgtgctggcc cacctgacgc aagatcgcga attcctgatg gcgcagatct
     1141 ggcccatcct gggcgaagtc caggccgaag gcccgtatgt ggcgcggcgc tacgaggcgc
     1201 cgggcggggc ctactcgctc caggtctttg tgtggcccgc gggcgcacag acacagatcc
     1261 acgaccattc gtgctggggc atcttcggcc cggtggcggg cacgttgcgc gaagagcgct
     1321 acctgcggct cgacgacggc gcccgcgccg acgaagccca cctgcgcgcc gactggcgcc
     1381 gcctctggac gccggacgac ggcctgtcca ccctgctgcc gtatgcgggc ggcgtccacc
     1441 gggtgagcaa ccgcagcctg cgccccgcca tctcgatcca cgtctacggc cccgcgggcg
     1501 ccatcgacgg gcgcgactat gaccccctgc gcgactacgt ctgcgaccgc ctgaccgacg
     1561 acacgccggg cgggctgctg ctggacgacc tcatcgccgc ctgctgatcc actagatccg
     1621 tcattctgag cgcagcgaag aatctccgtc ttgtactccc acgagacgag actcttcgct
     1681 ccgctcagag tgacaagccc tcggcgtcag gattgcggcg gcttgggatg cccggcgcgg
     1741 accggcacag tgccgtcgcg gaactggaga tcgaggatgt cgccgccggc gatgtcggcg
     1801 atggaggcgg cgacgtggcc ggtgcggcgg ttgcgcgcga tgacgtagcc gcgcgtgagg
     1861 atcgccgtgg gggccagggc ttgcaggcgg gcctgctgcg tggtcaggcc ggcgcgttgc
     1921 agggcaaggc gatggctcaa gtgcaggcgg gccgcctcgg ccaggctgtc gaggcgctgg
     1981 cgctgctgtt ccacctgggc gcgtggcgaa tggcgggcca gcacggcgcg ctgctcggcc
     2041 aggtcgcccg ccgcctgctc caggcagccg ttcatcaggt cccagagctc ctggcgcagg
     2101 gcttccacgt cgtcgatcag ttcgcggata tcgggcgcca ccagttcggc ggcggcggag
     2161 ggcgtcggcg cgcgcagatc ggctacgtag tccacgatgg tcgtatcggt ctcgtggccc
     2221 acaccggtca cgacaggcac gatggatgcg tagacggcgc gggccac
//
LOCUS       DASUYC010000214         3002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_16778,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000214 DASUYC010000000
VERSION     DASUYC010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16778"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..143)
                     /locus_tag="VF276_03440"
                     /pseudo
     CDS             complement(1..143)
                     /locus_tag="VF276_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012688574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
     gene            131..652
                     /locus_tag="VF276_03445"
                     /pseudo
     CDS             131..652
                     /locus_tag="VF276_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258918.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
     gene            complement(1061..2731)
                     /locus_tag="VF276_03450"
     CDS             complement(1061..2731)
                     /locus_tag="VF276_03450"
                     /inference="COORDINATES: protein motif:HMM:NF024598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HEX6605950.1"
                     /translation="MSRQRYFSDYGRGVSRREQRANIIPYLVIALLALLALVLTRVAP
                     PEAAINISGRVLDSYTGQPVVGANVAILAAGPDNGPAVASSSGVSIVGTQPVTVAGIS
                     TTTTTLPAPAPVVTTTSAADGGFTLLNLSVPNPVLRVEQPGYAPQQVPTGGRSGLDIR
                     LVPDTLHGTVSDAAGHAVVSATVAVGDQVATTGSDGSYTLSGIGTEHKLLVKAPGYAL
                     ARTQFGATTEQNVTLTPFAARAIYVNADTMASSGKFYNLIKLANDTEINAMVIDVKAD
                     TTGYVLYNSALPQVQAAGAANPMIGDLRSVIDTLHKNNIYVIARQALFWDEKLVAAHP
                     DWAIKSKSTTGPWQDAYGHHWANPYRQEVWDYNIAIAKEVASLGADEIQFDYVRFPSD
                     GNLADTDYGVPDSQTRASAITGFLQAAHKELSKSGAFIAADVFGLSPIVSDDLGIGQQ
                     FDELVQYLDYICPMAYPSHYADGFLGFDKPAEHPSEVVGYTLQQAQAKMLKTSARLRP
                     WLQDFTLGGIAYDATRVRGQIETAEQDDTSGWMLWNFDNVYTQAALKP"
     gene            complement(2768..>3002)
                     /locus_tag="VF276_03455"
     CDS             complement(2768..>3002)
                     /locus_tag="VF276_03455"
                     /inference="COORDINATES: protein motif:HMM:NF024598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HEX6605951.1"
                     /translation="AHPYEIIKQAGIYTGERMVGQRAKYRPWLQGFDWTHLVYDAHLY
                     RLQIQAADETGAAGWMFWDPSNVYSVGGFLPEP"
BASE COUNT          505 a          966 c         1031 g          500 t
ORIGIN      
        1 tcgatcaacg gacggatcag gtcgagcggg agcgggaaga cgatcgtcga gccctggttc
       61 gcgctgatct cggagagcgc ttggaggtag cgcagctgaa tcgtgaccgg gttcgggctg
      121 atcacctcgg ctgcctccgc aagaagtcat caccagagac aacgtgacgg tgcgcgtgaa
      181 cgccgtgctc tacttccagg taacggaccc tgggcgagcg gtaacacagg tcacggacta
      241 tatccgcgcc accttgcaga tcgcccagac gacgctacgt tcggtgctgg ggcagtcgga
      301 actggacgag atgctgtcgc accgcgagtc gatcaaccag cggttgcagc agatcatcga
      361 cgagcagacc gagccgtggg gcatcaaagt gaatatcgtc gaggtgaagg acgtggagct
      421 gccgcagtcg atgcagcgcg ccatggcccg ccaggcggaa gccgagcgcg aaaagcgcgc
      481 caagatcatc cacgccgacg gcgagttcca ggcgtcgcag atgctggccg acgcggccaa
      541 ggtgatcggc tcggagccgg cctcgatcca gttgcgctat ctgcaaaccc tgaccgagat
      601 cgccacggag cgcaactcga cgatcatctt cccggtgccg ctggaattgc tgagcctgat
      661 cccgcatgtc agttcggcgg ccgccgggct gatgggcgcg ggaaccgcga caccgccgat
      721 cccgcgtccg gaagtccggc ccgcaccggc gccggaggcg ctgccggcct cggtggcccg
      781 cgtgggcgcc ccgacccagg ccgcgccgcc gcccggcttc gccccgccgc cccagccggg
      841 cgccgcaggt ccgccgccgg gcgtcccgcg ccccccggcg caaggctagc accggcgccg
      901 ggcaccgggc ccggcgcaca aaagcggggc gggtggaagg ttccacccgc cccgttcgca
      961 tccccaacat cgggccggtt tcaaaccggc ccctacaatt agccctccgc tatcagcact
     1021 ctgtactcta cactcgtcgt aactcagcac tcagcactcg ctaaggcttg agggcggcct
     1081 gggtgtagac gttatcgaag ttccagagca tccagccgga ggtatcgtcc tgctcggcgg
     1141 tttcgatctg gccgcggacc cgcgtggcgt cataggcgat gccgcccagg gtgaagtctt
     1201 gcagccaggg gcgcaagcgg gcgctcgtct tgagcatctt cgcctgggcc tgctgcaagg
     1261 tgtagccgac gacttccgac gggtgctcgg cgggcttgtc gaagccgagg aagccgtcgg
     1321 cgtagtgcga cgggtaggcc atcgggcaga tatagtcgag atactgcacc agttcgtcga
     1381 actgctggcc gatgcccagg tcgtcgctca cgatggggct gaggccgaat acgtccgccg
     1441 cgatgaaagc accgctcttg gagagttctt tgtgggcggc ctgcaggaag ccggtaatgg
     1501 cgctggcgcg ggtctgggag tccggcacgc cataatcggt atcggccaga ttgccgtccg
     1561 aggggaagcg cacatagtcg aactggatct cgtcggcgcc gagcgaggcg acctccttgg
     1621 cgatggcgat attgtagtcc cagacttcct ggcggtaggg gttggcccag tggtggccgt
     1681 aggcatcctg ccaggggccg gtcgtggatt tgctcttgat ggcccagtcg gggtgcgcgg
     1741 ccaccagttt ctcgtcccag aacagcgcct ggcgggcgat gacgtagatg ttgttcttgt
     1801 gcagcgtatc gatcacgctg cgcaggtcgc cgatcatcgg gttggcggcg ccggcggcct
     1861 ggacctgggg cagcgcgctg ttgtagagca cgtacccggt ggtatcggcc ttcacgtcga
     1921 tgaccatggc gttgatctcg gtgtcgttcg ccagcttgat caggttgtag aacttgccgg
     1981 acgaggccat agtgtcggca ttaacataga tggcgcgggc ggcaaagggc gtcagggtga
     2041 cgttctgctc ggtggtcgcc ccgaactgcg tgcgggccag ggcatagccc ggcgccttca
     2101 ccagcagctt gtgctcggtg ccgatgccgg agagggtgta gctgccgtca ctgccggtgg
     2161 tggcgacctg gtcgccgaca gccacggtgg cgctcacgac cgcgtgcccc gcggcatcgc
     2221 tgaccgtgcc gtgcagcgtg tcgggcacca ggcggatatc gagcccgctg cgcccgccgg
     2281 tcggcacttg ctgcggtgcg tagccgggct gttccacgcg cagcaccggg ttgggcacgc
     2341 tcaggttgag caaggtaaag cccccatcgg cagcactcgt ggtggtgacg acaggggcgg
     2401 gcgcgggcaa cgttgtcgtg gtggtactga taccggccac ggtcaccggc tgggtgccca
     2461 caatggacac cccgctacta ctggcgaccg ccgggccgtt gtcgggcccg gcggcgagga
     2521 tcgccacatt ggccccgaca accggttgcc cggtatacga gtcgagcacg cgcccgctga
     2581 tattgatggc cgcctccggc ggggccaccc gcgtgaggac gagagcgagg agcgcgagca
     2641 gcgcgatcac caaatagggg atgatgttcg cgcgctgctc acgccgcgag acgccgcgac
     2701 cgtaatccga aaaatagcgt tgccgcgaca caaactcctc ctcctggcgg ggcgctgccg
     2761 ggcagcctta cggctccggc aggaacccac ccacggaata gacgttgctg ggatcccaga
     2821 acatccaccc cgcagcgccc gtttcatccg ccgcctggat ctgcaagcga tacaagtgcg
     2881 cgtcgtagac caggtgggtc cagtcaaacc cctgtagcca cggccggtat ttcgcccgct
     2941 gcccgaccat gcgctcgccg gtatagatgc cggcttgctt gatgatctca tacgggtgcg
     3001 ca
//
LOCUS       DASUYC010000215         2348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1689617, whole genome shotgun sequence.
ACCESSION   DASUYC010000215 DASUYC010000000
VERSION     DASUYC010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1689617"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /locus_tag="VF276_03460"
     CDS             <1..1088
                     /locus_tag="VF276_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605952.1"
                     /translation="HPQHQDPNDLWDWTRFMNMVKTRLDSAGYVDYYVEGPPTMSRRL
                     FHDTLAANPRSPITDDADRYYDLCMQDRVNPAVALAFFVKESSKGTAGVAVQTQSWGN
                     MRRARKAERTTGTLNTQWGPFAIYRTWLDSLADWCENLLSEVYKGEGRTTVRSVLPKY
                     APSFENDTALYIQQTITRISQWKDMPPGGDGSPGGFVDFAIKDQPTMSRGQFAAVLQQ
                     YNSPARADANTLYDLCVTNGVNPAVALAFFVQISRAGTAYANPAKANNHNWGEITGDG
                     SGAGGVQAYPGWPQGLVDWIRVIAGMYGSQGLTTLSTVVPRYRPGGTTDPAGFARALS
                     AMIQGWKAEYDQFAYSLGPPNNPMGGR"
     gene            1216..1410
                     /locus_tag="VF276_03465"
     CDS             1216..1410
                     /locus_tag="VF276_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605953.1"
                     /translation="MVNRITLRDPDLEDDDELDDEDFDDEDEDFDDEDEDFDDDEDFD
                     DEDEEDLEDEEDYDPEEEEV"
     gene            complement(1501..>2348)
                     /locus_tag="VF276_03470"
     CDS             complement(1501..>2348)
                     /locus_tag="VF276_03470"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEX6605954.1"
                     /translation="APWFAVSGLPISEAYWATVKIAGKPADVLIQAFERRVLTYVPSQ
                     PKDWQVEMGNIGLHYFEWRYGGGPAAQPGVSPPPGPPAGPPALAQIAITGIVVGKSGL
                     DLNEQSVTLVNRGAAPVLMTGWRLVSPKNDHVDIYTFPQDFTLPAGAQVVVHAGQGFD
                     GNGNLYMRRITWLFDATGFDGVILYDNLGREVTRYFLQQGAVPTAPAPAATAPPTATE
                     EPGAGVTDTPEPTDTPESGDITATATPGSRSGTPTATVTPGGTVTVTATPGARTATAT
                     ATATP"
BASE COUNT          437 a          762 c          797 g          352 t
ORIGIN      
        1 gccatcccca gcaccaggac cccaacgacc tgtgggactg gacacgcttc atgaacatgg
       61 tcaagacccg cctggacagc gccggctacg tggactacta cgtcgagggg ccgccgacga
      121 tgagccgccg gcttttccac gacacactgg ccgccaaccc gcgcagcccc atcaccgacg
      181 atgccgaccg gtactatgat ctctgcatgc aggaccgcgt gaacccggcg gtggcgctgg
      241 cctttttcgt gaaagagtcg agcaaaggca cggcgggcgt ggccgtgcag acgcaaagct
      301 ggggcaacat gcgccgggcg cgcaaggccg agcgcaccac cggcaccctc aatacccagt
      361 gggggccctt cgccatctac cggacctggc tcgacagcct ggccgactgg tgcgagaacc
      421 tgctcagcga ggtctacaaa ggcgagggcc gcacgacggt gcgcagcgtc ctgcccaagt
      481 acgcgccgtc gttcgagaac gacaccgcgc tctacattca gcagacgatc acccgcatca
      541 gccagtggaa ggatatgccg ccgggcgggg acggcagtcc gggcggcttt gtcgatttcg
      601 cgattaagga ccagccgacg atgagccggg gccaattcgc ggcggtcctg caacagtaca
      661 acagcccggc ccgcgccgac gccaatacgc tgtacgacct ctgtgtcacc aacggggtga
      721 atcccgccgt cgcgctggcg ttcttcgtgc agatcagccg cgcgggcacc gcctacgcga
      781 acccggccaa ggccaataac cacaactggg gcgagatcac cggagacggc agcggcgccg
      841 gcggcgtgca ggcctaccct ggctggccgc aggggttggt cgattggatc cgcgtcatcg
      901 cggggatgta cggcagccag ggcctgacca cgctgagcac cgtggtgccg cgctaccggc
      961 ccggcggcac gaccgacccg gcggggttcg cgcgcgcgct cagcgccatg atccagggtt
     1021 ggaaagccga gtacgatcaa tttgcctatt cgctaggacc acctaataac ccgatgggcg
     1081 ggagatagtt agtaatacgg gcgaccaagc gccgggtagg gccgcgtatg ccacgcgtca
     1141 cgcccgtcat cagcagcgag gagtgcggcc gccaaggcac cgcctcacta atcgactcga
     1201 aggagtgtgt ctgcaatggt aaaccgaatc acgctacgcg atccggattt ggaagacgat
     1261 gatgaactcg acgacgagga cttcgacgac gaggacgagg acttcgacga cgaggacgag
     1321 gacttcgatg acgacgagga cttcgacgac gaggacgagg aagacctgga ggatgaggaa
     1381 gactacgatc ccgaagaaga ggaagtctag gcgcgccgcg ccaatcgcat aagcgccaca
     1441 gaaacagggt cgtatgccgc agcatacgac cctgttttca atccggcccc ggttagccgg
     1501 tcaaggcgtc gcggtggccg tggcggtggc cgtgcgcgcg ccgggcgtgg cggtaacggt
     1561 caccgtgccg ccgggagtaa ctgtggcggt gggcgtaccg ctgcgcgatc ccggcgtggc
     1621 ggtcgcggtg atgtcgccgg attccggggt atcggtcggc tccggagtgt cggtcacgcc
     1681 ggcgcccggt tcctcggtcg cggtcggcgg tgcggtcgcc gccggggcgg gggccgtcgg
     1741 tacggcgccc tgctgcaaga agtagcgggt cacttcgcgg cccaggttgt cgtacagaat
     1801 cacgccgtcg aacccggtcg cgtcgaacaa ccaggtgatc cggcgcatgt acaggttgcc
     1861 gttgccgtcg aagccttgcc cggcatggac aaccacctgg gccccggcgg gcagtgtgaa
     1921 gtcctgcgga aatgtgtaaa tatcgacgtg gtcgttcttg gggctgacca gccgccagcc
     1981 ggtcatgagc accggcgcgg caccacggtt caccagagtc accgactgct cgttcagatc
     2041 gaggccactc ttgcccacga cgatgccggt gatggcgatt tgcgccaggg cgggcggccc
     2101 ggcgggcggg ccgggcggcg gcgacacgcc gggttgtgcc gcgggcccgc cgccgtagcg
     2161 ccattcaaaa tagtgcagcc cgatgttgcc catttccacc tgccaatcct tcggctggct
     2221 gggcacatag gtcagcacac ggcgctcgaa cgcctggatc agcacatcgg ccggtttgcc
     2281 cgcgatcttg actgtggccc agtacgcctc gctgatcggc agcccgctga ccgcgaacca
     2341 gggcgcga
//
LOCUS       DASUYC010000216         6987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1948511, whole genome shotgun sequence.
ACCESSION   DASUYC010000216 DASUYC010000000
VERSION     DASUYC010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6987
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1948511"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="VF276_03475"
     CDS             <1..1140
                     /locus_tag="VF276_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014209714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX6605955.1"
                     /translation="ESYVEDVPAATVAALAERGRLAATTDYAALAVCDAVSICVPTPL
                     GKTGDPDISYIVSASQEVARYLHPGMVVVLESTTYPGTTTEVLLPLLEGNGQSLRAGE
                     DFFLAFSPERVDPGREDWTTHTTPKVIGGVTPACLQVAMAYYSSAIQTLVPVSSTEAA
                     EMVKLLENTFRAVNIGLVNEVLLMADKLGLDIWEVIDAAATKPFGFMKFTPGPGLGGH
                     CIPIDPLYLSWKMKTLNYTARFIELASEVNTNMPRYWVQKVQDALNDAGKPVKGSRIL
                     VLGVAYKKNISDVRESPALDIIHLLSEKGAQVSYHDPHVPAFHHEGMEMASVTDLDAA
                     LTASDCVVIATDHAAYDWARIGERAQLIVDTRRAVRPVPAPAGAA"
     gene            1395..3200
                     /gene="lepA"
                     /locus_tag="VF276_03480"
     CDS             1395..3200
                     /gene="lepA"
                     /locus_tag="VF276_03480"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEX6605956.1"
                     /translation="MPDQSHIRNFCIIAHIDHGKSTLADRLLQTTGTVAERDMVAQVL
                     DSMPLEREKGITIKARAVRMRYQATDGAEYELNLIDTPGHVDFTYEVSRSLAACEGAV
                     LVVDAAQGIEAQTLANVYLALEHDLSLVPVVNKIDLPSAQPELVAMEIDRVIGLPMDE
                     VIFASAKEGRGITEILEAIVKHVPPPTGAHRRPLRALIFDSHYDPYKGVIAYCRIVDG
                     EVKEGDRVQMMATGAGAEVLEIGVFTPAMTATGVLRTGEVGYVATGLKGISECQVGDT
                     ITLVAQPADEPLPGYRPVKPMVFAGIYPVETVEYQNLRDALDKLHLNDASLVYQPESS
                     TALGFGFRCGFLGLLHMEIIQERLEREYGLELITTAPSVEYRITKTDGSVIVVDNPAE
                     MPDAASIRQVEEPWMAIQVVTPTRFIGAIMELVTNRRGIYTKTEYLDETRVSLAYDVP
                     LSEMIVDFYDQLKSRTQGYASLDYSFSDYQPADLVKLDVLVNGVPVDALSVITHRDRA
                     ISQGRALVDKLRRLIPRQMFDVPIQAAIGSKIIARETISAMRKNVLAKCYGGDISRKR
                     KLLEKQKEGKARMKTVGSVDIPQEAFLAMLSLDED"
     gene            3277..4602
                     /locus_tag="VF276_03485"
     CDS             3277..4602
                     /locus_tag="VF276_03485"
                     /inference="COORDINATES: protein motif:HMM:NF025009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEX6605957.1"
                     /translation="MTFQQRPSYDDEELSPGGAPAPRRAEKARRPVPQPLDEALEGEE
                     AYLAEDEPMAAEPAGSVVDAADGTLEDEEGEPAPAPVRVRPAASAPRRPAPPQPAAKP
                     PAAKPAPPKSTARPAARTASPARPAAPAPRTPAPRSAKAAGRTRPAPTGGGIPRWLMI
                     ALVAVVLLGATGAVAVMGNNNPGGAPAGTPVSSAPPQSSGDVAATVNGKPISKAAWQE
                     RVRLAKQDYIDQFQLNFDNDQGKRMEDVLSFDVLDQMINFEILMQEAQRQGIVTNPTQ
                     VEDQYQKIRGNIFKQEAEKIAPSPNPTQVEQRLQQINANPDEQARIWSDFLKSQNMTS
                     DAQFRQTIIDSFIYLIMATNHTTPGATDDEKTTQFANYICSTREKYDVKILVTFIVPQ
                     TPCESAGASAPGALPSLSTVPGSPVPTQAVPPAPVETGEPAGLTPGAQP"
     gene            4606..5415
                     /locus_tag="VF276_03490"
     CDS             4606..5415
                     /locus_tag="VF276_03490"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX6605958.1"
                     /translation="MDDTTRALARRTAVEAATAAAAIAHEGFLAPRQITAKGHNDIVT
                     EIDLAAEETICRALGAAFPADGILAEERGQSAGDSPYLWIIDPLDGTHNYANQLPFWC
                     VSIARYNSETRQVELGVIADPLHGETYVAERGQGATLNGRPIHVSTLTDMRQSIIGCD
                     IGYAQTISARMLRTAVQVQPDVRRLRILGSAVLAMAYVACGRLDMFFHLHLQPWDLAA
                     AWLLIEEAGGRCTNWRGEPTTLEDREIVAGPPALQRALIDRLLNLGEDHAS"
     gene            5412..6248
                     /locus_tag="VF276_03495"
     CDS             5412..6248
                     /locus_tag="VF276_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605959.1"
                     /translation="MSGPRLLVILLAALVLGGALLACDAMPGPQPTPTVAGTPLPTAP
                     PTDTPAPTTTPVPTNTPVPTPTEGAVRTPVGGPAGPEFMAALAAMDAAPTYSYTLRME
                     LGPTSTQYLLTGSGRHQPPADYDTNYSALGYHTEIAVISGTTYFRNFGVWKQGLPDAT
                     QFPMGPPPDIPAILGLLTYAADAGLAPEGSDGPDAPSPRHLQFRLDPDGLSGPGGPGL
                     GTSGGDLWVDPATHRYVRLILHFGRPDTPADNAGQLQMDFLDYGAPVTLTPPALPSPA
                     GN"
     gene            6407..6805
                     /locus_tag="VF276_03500"
     CDS             6407..6805
                     /locus_tag="VF276_03500"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017366483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX6605960.1"
                     /translation="MKEYRNPANVHPPVAGYTHQIEVREPARWLVLSGQIGKREDGTV
                     PTAPVEQLDVALDNVIRNLQAAGMDLPDLVKLTFYLVGDMDPQRRREVTAARLNGHQP
                     CMTVLVVAALAHPDYKVEIDAWASSSDPGR"
BASE COUNT         1267 a         2488 c         2227 g         1005 t
ORIGIN      
        1 gaatcgtatg tcgaagacgt gcccgccgcc accgtggcgg ctctggcgga gcgcgggcgg
       61 ttggcggcca cgaccgacta cgcggccctg gccgtgtgcg acgccgtctc gatctgcgtg
      121 ccgacgccgc tgggcaagac cggcgacccg gacatttcgt acatcgtctc ggcctcgcag
      181 gaggtggcgc gctatctgca ccccggcatg gtcgtcgtgc tggagtcgac tacctacccc
      241 ggcaccacca ccgaggtgct gctgccgctg ctcgaaggca acggccagag tttgcgcgcc
      301 ggggaagact ttttcctggc cttctcgcct gagcgggtgg acccagggcg tgaggactgg
      361 accacgcaca ccacgcccaa ggtcatcggc ggcgtaaccc cggcctgcct ccaggtggcg
      421 atggcctact acagcagcgc catccagacg ctcgtgcccg tctcgtccac cgaggcggcg
      481 gagatggtca agttgctgga gaacaccttc cgcgccgtca acatcggcct ggtcaacgaa
      541 gtgctgctga tggccgacaa actgggcctc gatatctggg aagtgatcga cgcggcggcc
      601 accaagccct tcggcttcat gaagttcacg ccggggccgg gcctgggcgg ccactgcatc
      661 cccatcgacc cgctctacct gagctggaag atgaagacgc tcaactacac cgcgcgcttc
      721 atcgagttgg ccagcgaggt caacacgaat atgccgcgtt actgggtgca gaaggtgcag
      781 gacgcgctca acgacgcggg caagccggtc aagggtagcc gcatcctggt gctgggcgtg
      841 gcctacaaga agaacatcag cgacgtgcgc gagtcgccgg cgctcgacat catccatctg
      901 ttgagcgaga agggcgcgca ggtgagctac cacgacccgc acgtgcccgc cttccaccac
      961 gagggtatgg agatggcaag cgtgaccgat ctcgacgcgg ccctgacggc gagcgattgc
     1021 gtggtcatcg ccaccgacca cgccgcctac gattgggcgc ggatcggcga gcgcgcgcaa
     1081 ctgatcgtgg acacgcggcg ggcggtgcgc ccggtgccgg ccccggcggg cgcagcctga
     1141 cacctgcacg taagcggtgc caatttcacc gcgaaggcgc gaaggacgcg aagaatagga
     1201 ctgaaataaa caaccttcgt gtccttcgcg ccttcgcggt gcatttacgc ctggatagca
     1261 ggggcgcggc ttgacaaagg tgggccgcct gcctatacta aaaattaccg aaccccgccc
     1321 ggttgccgcg cggggtgtga tacgtgccaa tgttgggcgg ccgaggtcgg ccgcctagtt
     1381 tgtgttagga taccgtgccc gaccaatccc acatccgcaa tttctgcatt atcgcgcata
     1441 tcgaccacgg caaatcgacg ctggccgacc ggctgctgca aaccaccggc acggtcgccg
     1501 agcgcgacat ggtggcgcag gtgctcgaca gcatgccgct ggagcgcgaa aaaggcatca
     1561 ccatcaaggc gcgcgccgtg cgtatgcgct accaggccac cgatggcgcc gagtatgagc
     1621 tgaacctcat cgacacaccg gggcatgtgg acttcaccta cgaagtgagc cgcagcctgg
     1681 ccgcctgcga gggcgccgtg ctggtcgtgg acgcggcgca gggcatcgag gcgcagacgc
     1741 ttgccaacgt ctacctggcc ctggagcatg acctgagcct ggtgcccgtc gtcaacaaga
     1801 tcgacctgcc cagcgcgcag cccgagttgg tcgccatgga gatcgaccgg gtgatcgggc
     1861 tgcccatgga cgaggtgatc tttgcctcag ccaaagaggg gcgcggcatc accgagatcc
     1921 tggaggccat cgtcaagcat gtgccgccgc ccaccggcgc ccaccgccgc cccttgcgcg
     1981 ccctgatctt cgacagccat tacgatccct acaaaggcgt catcgcctac tgccgcattg
     2041 tggacggcga ggtgaaagag ggagataggg tccagatgat ggcgaccggc gccggggccg
     2101 aagtgctgga gatcggcgtg ttcaccccgg ccatgaccgc caccggcgtg ctgcgcaccg
     2161 gcgaggtggg ctacgtggcg acgggcctca agggcatcag cgagtgccag gtgggcgata
     2221 cgatcacact ggtcgcccag cccgccgacg aaccgctgcc cggctaccgc cccgtcaagc
     2281 cgatggtctt cgccgggatc tacccggtcg agacggtcga gtaccagaac ctgcgtgacg
     2341 cgctcgacaa gctgcacctc aacgacgcct cgctggtcta ccagccggag agcagcacgg
     2401 cgcttggctt cggcttccgc tgcgggttcc tgggcctgct gcatatggag atcatccagg
     2461 agcgcctgga gcgcgagtac ggcctggaac tgatcaccac cgcgcccagc gtggagtacc
     2521 gcatcaccaa gaccgacggc agcgtgatcg tcgtggacaa ccccgccgaa atgcccgacg
     2581 ccgccagcat ccggcaagtc gaggagccgt ggatggcgat ccaggtcgtc accccgactc
     2641 gttttatcgg cgcgatcatg gaactggtga ccaaccggcg cggcatctac accaagaccg
     2701 agtacctcga cgagacccgc gtctccctgg cctatgacgt gcccttgagc gagatgatcg
     2761 tcgatttcta cgaccagttg aagtcgcgca cgcagggcta tgcctcgctc gactacagct
     2821 tctccgatta ccagcccgcc gacctggtga agctcgacgt gctggtgaac ggcgtgccgg
     2881 tcgacgcgct cagcgtgatc acgcaccgcg atcgggccat cagccagggc cgcgcgctgg
     2941 tcgataagct gcgccgcctg atcccgcgcc agatgttcga cgtgcccatc caggcggcca
     3001 tcggcagcaa aatcatcgcc cgcgagacca tctccgccat gcgcaagaac gtgctggcga
     3061 agtgctacgg cggcgacatc tcgcgcaagc gcaagctgct cgaaaagcaa aaagaaggaa
     3121 aggcccggat gaagaccgtg ggcagcgtcg atattccgca agaagccttc ctcgccatgc
     3181 tcagcctgga cgaggactga ccgtccaccg gcggctccag gcccggcgca ttgcaaccgg
     3241 acgccattgg gccggagccg catatcaggg aaccgtatga cctttcaaca acgaccgagt
     3301 tacgacgacg aagaactgtc tccgggaggc gcgcccgccc cacgtcgcgc cgagaaggcc
     3361 cggcgccccg tcccccagcc gctagacgag gcgctggagg gcgaggaggc gtatctcgcc
     3421 gaggacgagc cgatggcggc ggagcctgcc ggatcggtcg tggatgcggc ggacggcacg
     3481 ctggaagacg aagaaggcga gcctgcgccc gccccggtgc gggtccggcc cgctgcgtct
     3541 gcgccccgcc gtcccgcccc gccgcaaccc gccgccaaac cgcccgcagc gaaaccggcg
     3601 ccgcccaaat ccaccgcccg gcccgccgcc cgcaccgcga gcccggcccg cccggcggcc
     3661 cccgcccccc ggacgcccgc cccgcgcagc gccaaggccg ccgggcgcac ccgcccggcc
     3721 ccgaccggtg gcggcatccc gcgctggtta atgatcgccc tcgtggccgt cgtcctgctg
     3781 ggcgccaccg gcgccgtcgc tgtcatgggc aacaacaacc ccggcggcgc gcccgcgggc
     3841 acgcccgtct ccagcgcccc gccgcagagt tcaggcgatg tggccgccac cgtgaacggc
     3901 aagccgatct cgaaagcggc gtggcaggag cgtgtccggc tcgccaagca ggactatatc
     3961 gaccagttcc agttgaattt cgacaacgac cagggcaagc gcatggagga tgtgctgtcc
     4021 ttcgatgtgc tcgatcagat gatcaacttt gagatcctca tgcaggaggc acagcgccag
     4081 gggatcgtca ccaaccccac ccaggtcgag gaccagtacc agaagatccg cggcaacatc
     4141 ttcaagcagg aagccgagaa gatcgcgccc tcgccgaacc cgacgcaggt ggagcagcgc
     4201 ctgcagcaga tcaacgccaa tcccgacgag caggcgcgga tctggagcga ctttctcaag
     4261 agccagaaca tgaccagcga cgcgcagttc cgccagacga tcatcgacag cttcatctac
     4321 ctgatcatgg cgacgaacca cacgacgccc ggcgccaccg acgacgagaa gacgacgcag
     4381 ttcgccaatt acatctgctc gacgcgcgag aaatacgacg tgaagatcct ggtcacgttc
     4441 atcgtgccgc agacgccgtg cgagagcgcc ggtgcctctg cccccggcgc gttgccgagc
     4501 ctgagcaccg tgccgggcag cccggtgccg acccaggccg tgccgcccgc gcccgtcgaa
     4561 acgggcgaac cggcgggcct gacgccgggg gcgcagccgt aagccatgga tgataccacc
     4621 cgcgccctgg cccgccgcac ggccgttgag gccgccaccg ccgccgccgc gatcgcccat
     4681 gagggcttcc tggccccccg gcagatcacg gccaagggcc acaacgacat cgtcaccgag
     4741 atcgacctgg cggcggagga gacgatctgc cgggcgctgg gcgccgcctt ccccgccgac
     4801 ggcatcctgg ccgaggagcg cggccagagc gcgggcgaca gcccctatct ctggatcatc
     4861 gatccgctgg acggtacgca caactacgcc aaccagttgc ccttctggtg cgtgtctatc
     4921 gcccgctaca actccgagac gcggcaggtg gaactgggcg tgattgccga cccgctgcac
     4981 ggcgagacct acgtagccga gcgcggccag ggcgcgacgc tgaacgggcg ccccatccac
     5041 gtcagcaccc tcaccgacat gcgacagagc atcatcggct gcgacatcgg ctatgcgcaa
     5101 acgatctcgg cccgcatgtt acgcactgcc gtgcaggtcc agcccgacgt gcgccgcctg
     5161 cgcatcctcg gctctgccgt gctggcgatg gcttatgtgg cgtgcgggcg gctcgatatg
     5221 ttcttccacc tgcacctgca accgtgggac ctggccgccg cctggctgct gatcgaggaa
     5281 gcgggcggcc gctgcaccaa ctggcgcggc gaaccgacca cgctggagga ccgcgagatc
     5341 gtggccgggc cgcccgcctt gcagcgcgcg ctgatcgacc ggctgctcaa cctgggcgag
     5401 gaccacgcgt cgtgagtgga ccccgcctgc tcgtgatcct gctggcggcg ctggtgctgg
     5461 gcggcgcgct gctggcctgc gacgccatgc cggggccaca gcccacgccg acggtggcgg
     5521 gcacgccgct gcccaccgcg ccgccgaccg atacccccgc gccgacgacc acgccggtgc
     5581 ccaccaacac gccggtgccc acgcccaccg agggcgctgt ccgcactccg gtcggaggcc
     5641 ccgccgggcc cgagttcatg gccgccctgg ccgcgatgga tgccgcgccc acctacagct
     5701 acaccctgcg catggagctt gggccgacaa gcacgcagta cctgctgacc ggcagcgggc
     5761 gccaccagcc gccggcagac tacgacacga actacagcgc gcttggctac cacaccgaga
     5821 tcgcggtgat cagcggcacc acctatttcc gtaacttcgg cgtctggaag caagggctgc
     5881 ccgatgccac gcagttcccc atggggccgc cacccgacat ccccgccatc ctgggcctgc
     5941 tcacctatgc cgccgacgcc ggcctggccc cggagggcag cgacggcccc gacgcgccct
     6001 cgccgcgcca cctgcaattc cggctggacc cggacggtct ctccggcccc ggcggtccgg
     6061 gccttggcac gtccggcggc gacctctggg tcgatcccgc gacgcaccgc tacgtccgcc
     6121 tcatcttgca cttcgggcgg cccgataccc cggcggacaa cgctggccag ttgcagatgg
     6181 acttcctcga ctacggcgcg cccgtcaccc tcaccccacc cgcgctgccg tcccctgccg
     6241 gcaattgagg gccggcgggt tacgacactc ggcggcccgg ctcttgaagc gcccacccgg
     6301 cccttatcca aaatagcgga atagccccgg ccccgcattc gacggcaagc acgcggcgcc
     6361 ggtgatgcac ctggccgcat ggacatggaa aggaaggagc aacgccatga aggagtatcg
     6421 caaccccgcc aacgtccacc cgccggttgc cggatatacg caccagatcg aggtgcgcga
     6481 acccgcacgc tggttggtcc tgtccgggca gatcggcaaa cgggaagacg gcacggtgcc
     6541 cactgcccca gtggagcaac tcgatgtggc gttggacaac gtcatccgca acctccaggc
     6601 agctgggatg gacctgcccg acctggtgaa actgaccttt tacctcgtgg gcgacatgga
     6661 cccgcagcgg cgccgcgagg tgaccgccgc gcgcttaaac gggcaccaac cctgcatgac
     6721 ggtgctggtc gtggctgcgc tggcgcaccc ggactacaag gtcgagatcg acgcctgggc
     6781 cagtagttcc gaccctgggc gttagcacca tggtgcctgc agcgttaata tagcgggccc
     6841 cagaacgacc cggaataccc atgcgcactc gcatcagttc ctgggttcac cgcgaggaac
     6901 gaagaacgcg aagattattg atataggttt tattcttccc atccttcgcg ccttcgcggt
     6961 gcagaataag ctatctgaac cgatgcc
//
LOCUS       DASUYC010000217         3490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_282264,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000217 DASUYC010000000
VERSION     DASUYC010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282264"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..294
                     /locus_tag="VF276_03505"
     CDS             112..294
                     /locus_tag="VF276_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605961.1"
                     /translation="MYTNEYGLQRLALETPQARVREARQAERAQIALHAHQAPAVTAS
                     QPRLARLLAGLHLTTR"
     gene            560..1102
                     /locus_tag="VF276_03510"
     CDS             560..1102
                     /locus_tag="VF276_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605962.1"
                     /translation="MSVQNIDPEFKVYRPVDEAILTAQAVAGLYGRAQWRGKLYRLFA
                     RLTRQGYRLRDLAAAEAEATITRRYYAGTQTVPLAAIRGSESRGADFDAWFHPLRPHD
                     RNRWLSVATAWHQGLDLSPVELIQMGTHYFVRDGHHRISIAAALGVRDIEANVTVWQM
                     AVPAPQPVCSPVACAEPSAA"
     gene            1289..2698
                     /locus_tag="VF276_03515"
     CDS             1289..2698
                     /locus_tag="VF276_03515"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6605963.1"
                     /translation="MSNVTLDEVPDRAAPAAADAGPPPAPAAQEAPPIPPEAGPGPSL
                     RGARDFWLVWSGQSVSLLGDGLYGIAMAWWITQTLGSATALAGYALCWFIPTITLPFV
                     AGSLIDRANRKALIALMDGLQGLAVTVLALLLATNRLELWHVYAGAVVLAACGAIHGP
                     TLDSSIPNLVPAAALVRANGLYATSGSVANIAGPLLGGTLVGFAGLPVAMVLNALSFW
                     FATGATLLARIPSPRVRDGGEKSAFARLLDDATYGYRWIWRHRAILYLLLIFTAGNFL
                     IAPTFPLETLVVKNQLAAGGAALGWDGAFIFGLISAAMAVGALVGAVFFSRGWAIKPM
                     AWGVCLGWAAGGLGALGFGLSTAVWLSVGLAFLIGASEPLCNIPSQTIWMSYTPDSER
                     GRIFAARRTIAWGIQPISIALGGIVAEQIGVGTLYVVAGALVTLIALGNLVFNRTIRT
                     LYAPDAAPEAANRGLLYRA"
     gene            complement(2752..2826)
                     /locus_tag="VF276_03520"
     tRNA            complement(2752..2826)
                     /locus_tag="VF276_03520"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2792..2794),aa:Met,seq:cat)
     gene            3185..>3490
                     /locus_tag="VF276_03525"
     CDS             3185..>3490
                     /locus_tag="VF276_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605964.1"
                     /translation="MPTTPAPPGSATRSERVRVRLAAVLLVLLVSAGLLAGALAYFMR
                     PVLLPINGRATLGPPVCGAWQVSTGPRALFGPPEDHISGVAVAADEAWAVGHSGLRRG
                     "
BASE COUNT          534 a         1255 c         1136 g          565 t
ORIGIN      
        1 gggtcccgat gcgcggatca cgggccggtg ctaagatagc atcatcaacg acagcaacca
       61 ccgcccggac gcggtaccag cggccaccga gccgagagaa aggaacccac gatgtatacc
      121 aatgaatacg ggttgcagcg cctggccctg gagactccgc aggctcgcgt ccgcgaagcc
      181 cgccaggcag aacgcgcgca gatagccctc catgcccatc aggcgcccgc ggtcactgcg
      241 tcccagccgc gcctggcccg gctcctggcc gggttgcact tgaccactcg ctaaccaaca
      301 gcgcgcttcc ctcccagaat gatggtaggc cgggtgatac acccggcctg ctgcactttc
      361 ccccgcatcc gcgcgctacc ccgaccggac aaaggccccc gcccggctcc gtctcccgat
      421 ggggaaaaac agttcgcaag ttccgcgtcg ggaccgacct ccatttgccc atcgcgaccg
      481 atgtgctccg gcgcattgtc ggctatagta gctatggaga cgaacacgca tacctggaca
      541 tgaaagggag tcgtagactg tgtcggttca gaatatagac ccggagttca aagtctacag
      601 gccggtggat gaggcgatcc tcaccgcgca ggccgtggcc ggcctgtacg ggcgcgccca
      661 atggcgcggg aaactctacc ggctcttcgc gcgcctgacc cgccagggct accggctgcg
      721 cgacctggcg gccgccgaag ccgaggccac catcacccgc cgctactacg ctggaacgca
      781 gacggtgccg ctggcggcca ttcgcggcag cgagagccgc ggcgccgact tcgacgcgtg
      841 gttccacccg cttcgcccgc acgaccgcaa ccgctggttg agcgtcgcca ccgcctggca
      901 ccagggcctg gatctgtcgc ccgtcgaact gatccagatg ggtacacact acttcgtccg
      961 cgacggccac catcgcatct ccatcgccgc cgcgctgggc gtgcgcgaca tcgaggccaa
     1021 cgtaaccgtc tggcagatgg ccgtgccggc gccgcagccg gtgtgcagcc cggtagcgtg
     1081 cgcggagccg tctgcggcat aggcggctcc ggcccggcgc gccccggttt ttcggcgcag
     1141 cggcccgcgt gatccacccc gcgccccccc ttattccatc cccagacgga tagaccccac
     1201 ccgattctcc cgctataatc aaggtagccc gttcagcgct cgaagccgcg gccaggtgca
     1261 acgcccgctg ggcgaaggac atcgcactgt gagcaacgtc actctcgacg aggttcccga
     1321 ccgcgccgcg cccgccgccg cggacgccgg tcccccaccc gcgcctgccg ctcaagaagc
     1381 accgcccatc cccccggaag ccggccccgg cccctcgctg cggggtgcgc gcgatttctg
     1441 gctggtctgg agcgggcaga gcgtctcact gctgggcgac ggcctctacg gcatcgccat
     1501 ggcctggtgg atcacccaga ccctggggtc ggcgacggcg ctggccgggt atgcgctctg
     1561 ctggttcatc cccacgataa cgctgccctt cgtcgccggg tcgctgatcg accgggccaa
     1621 tcgcaaggcg ttgatcgcgc tcatggatgg cctgcaaggg ctggccgtca ccgtgctggc
     1681 gctcctgctg gcgacaaacc gattggaact ctggcacgtc tacgcgggcg cggtggtgct
     1741 ggccgcctgc ggcgccatcc acggccccac gctcgactcc tcgatcccca acctggtgcc
     1801 cgccgccgcg ctggtgcgcg ccaacggcct ctatgccacc tccggcagcg tcgccaacat
     1861 cgccgggccg ctgctgggcg gtaccctggt cggcttcgcc gggctgccgg tggcgatggt
     1921 gctcaatgcg ctcagcttct ggttcgccac cggcgccacc ctgctggcgc gcatcccctc
     1981 gccgcgcgtg cgcgacggcg gcgagaaaag cgccttcgcc cggttgctgg acgacgccac
     2041 ctacggctac cggtggatct ggcgccaccg ggccatcctc tacctgctgc tgatcttcac
     2101 cgccgggaac ttcctcatcg cgccgacctt tcccctggag acgctggtcg tgaagaacca
     2161 gcttgcggcc ggcggggcgg cgctgggctg ggacggcgct ttcatcttcg ggctgatcag
     2221 cgcggcgatg gcggtgggcg cgctggtggg cgcggtgttc ttcagccgcg gctgggcgat
     2281 caagccgatg gcctggggcg tctgcctggg ctgggcggcg ggcggcctgg gcgcactcgg
     2341 cttcggcctc tcgacggcgg tctggctctc ggtcgggctg gcgttcttga tcggcgcctc
     2401 ggagccgctc tgcaacatcc ccagccagac catctggatg tcgtacacgc ccgacagcga
     2461 gcgcgggcgc atcttcgcgg cgcggcgtac catcgcctgg ggcatccagc cgatctcgat
     2521 tgccctgggt ggcatcgtgg ccgagcagat cggcgtcggc acgctctacg tcgtggcagg
     2581 cgcgctggtt acgctgatcg cgctgggcaa cctggtcttc aatcgcacca tccgcacgct
     2641 ctacgcgccc gacgcggcgc ccgaagcggc caaccgcggc ctgctctacc gcgcctgacc
     2701 cagcagccag gcaaccaaaa aagaggcccc gtttccgggg cctcttatcg ttggtagcgg
     2761 aggcaggatt cgaacctgcg acctttgggt tatgagccca acgagctgcc actgctccac
     2821 tccgcgccgc tatggtcaaa gtatatcgca tcgcaggcca aatgtcaagg cgttcgcgtg
     2881 atatccaagc cgaaatttca ccgcgaaggc gcgaagagcg cgaagaatac ccccaatact
     2941 aataatcttc gcgcccttca ctgcaagtgg gaaggatggg tagccctggg aactgtcatt
     3001 ctgagcgcag cgaagaatct cgtctcgttg gggagcacga cgagactctt cgctgcgctc
     3061 agagtgacac gctacctttc atgccctcca gtgtcccacc gggacacacc acacttcgcg
     3121 gttaataaac gtcgcgcctg ggggcgtggc agatgggcgg cgttgtggta tagtggggcc
     3181 accgatgccc acgacccctg ccccacccgg ctccgcgacg cggagcgagc gcgtccgagt
     3241 ccggcttgcc gccgtcctgc tcgtgctgct ggtgagcgcc gggctgctgg cgggggcgct
     3301 cgcgtacttt atgcgcccgg tgctgctgcc gattaacggg cgggccacgc tgggcccgcc
     3361 ggtctgcggg gcgtggcagg tgagcaccgg gccgcgcgcg ttgttcggcc cgccggaaga
     3421 ccacataagc ggcgtcgccg tggcggcgga cgaggcctgg gccgtaggcc acagcggctt
     3481 gcggcgcggg
//
LOCUS       DASUYC010000218         3352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1705072, whole genome shotgun sequence.
ACCESSION   DASUYC010000218 DASUYC010000000
VERSION     DASUYC010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1705072"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VF276_03530"
     CDS             <1..240
                     /locus_tag="VF276_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605965.1"
                     /translation="RNAAGEWHRARREGRRGQAYWYVACRVGGRVQRFYLGPAPAVDG
                     DRLAAVAATIAAARAATVPAPPREEVRSRQPAESA"
     gene            593..1585
                     /locus_tag="VF276_03535"
     CDS             593..1585
                     /locus_tag="VF276_03535"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6605966.1"
                     /translation="MDVEAARALAPWISALASGLAFAGLAIAVYLTFRVLDFPDLTVD
                     GSLGFGASVTAALISFGHWNPWLTLVAATVAGALAGLATGLLHTALKINGLLASILVA
                     ISMFTINLRVMNGSNISLFNADTIYSGANGDPGVLGAWLAPFGALAARGDFRASVVLL
                     IMTAIMVALCFWWLNSEQGIALRATGDNPRMIRALGVNTDHMKILGLTVANGLCGFSG
                     AVLAQQLSYSDITLGPGAILTGLAAVILGEALIHGSGVLAGLAGAVVGAIVFQLIIAA
                     TQVQQVFTIEPTDYKLASALIVLIALLVPAVRSRLGMRSLARTRRSSAGKAGAP"
     gene            1582..2442
                     /locus_tag="VF276_03540"
     CDS             1582..2442
                     /locus_tag="VF276_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013514450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6605967.1"
                     /translation="MTTSPTTAQPAPAPAPRPAGRPALALRGVVKTFYPGSVNEVRAI
                     RGIDLSLQPGEFVTIIGGNGAGKSTLLNLIAGVFPPDSGTITVGGHECTNLSEDRRAR
                     WIGRVFQDPLAGTAPNLTIEQNLAMALLRGRGRGLGRGVTDSRRAIFREQLARLGIGL
                     ENRLHTRAGLLSGGQRQALTLLMATLQKPDVLLLDEHTAALDPATAATIVDLTARLIA
                     DAHLTALMVTHNMSLALHVGTRTLMMHEGQVILDLAGAERQAQTVRSLVDRFFALRGT
                     EVASDRMLLY"
     gene            2501..>3352
                     /locus_tag="VF276_03545"
     CDS             2501..>3352
                     /locus_tag="VF276_03545"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HEX6605968.1"
                     /translation="MNSAHTKTRAGLPRKGAFLPWLSLVLALGLLLSACGPTPDTGGP
                     TPATGATATTATGGTQATATTATGGEATATSAPSGEATATTATGGATATSAPSGPTAT
                     TATGGTQATATTGSATGKHYTVGILQTATHPALDFTREGAKQAFIQAGMVEGQTVTFD
                     LRNAELDAPTAAKMADAFIANKVDAIITIGSLASQAALAEEQKQGSTIPLFFTAVADP
                     YVAKLAGKQEGDTITADPKIHPAFETGVQAFPPVEDGLKLIKEVNPDVKTVGLLWNPN
                     EPNSKATT"
BASE COUNT          536 a         1198 c         1113 g          505 t
ORIGIN      
        1 cggaatgcgg cgggcgagtg gcatcgggcg cggcgggaag ggcgccgcgg ccaggcgtac
       61 tggtacgtgg cctgccgggt gggtggacgg gtgcagcggt tctacctggg gccggcgccg
      121 gcagtggacg gggaccgctt ggcggcggtc gcggccacta ttgccgccgc ccgggccgct
      181 acggtccccg cgccgcccag agaggaggtg agatcgcgcc agccggcgga atccgcctga
      241 gcggcccggg cggcagggac caggagagtc ggggtggtac actttgggtg aaaacctgac
      301 cggctggtac actttgggtg aaaacccgtg gggtggcaca ctttgggtga aaacctgacc
      361 ggctggtaca ctttggatgc aaacgggtgg ccggctgtgg gtgaaaaccg gtggtacact
      421 ccaggcgcaa cttgacactc ggcgtctctg cggtacgaat ccgtattctg accggatctc
      481 aggggccgcc tattgcattt gcggcggtgc ctcgctataa tggcggaagt atcgtgcaca
      541 gttatgtcat gccacagccg ggttcgcgca ggccggcgga ggtgagcgac gattggatgt
      601 tgaagccgcg cgggcgctgg cgccctggat tagcgcattg gcgagcggcc tggcctttgc
      661 cgggctggcc atcgcggtct acctcacctt ccgcgtcctc gacttccccg atttaacggt
      721 tgacggcagc ctgggtttcg gcgcctccgt caccgccgcg ctgatcagct tcggccactg
      781 gaacccctgg ctcaccctgg tggccgccac ggtagccggg gccctggccg gcctcgccac
      841 cggcctgctg cacaccgcgc tgaagatcaa cggcctcctc gcttccatcc tcgtcgctat
      901 cagcatgttc accatcaacc tgcgcgtgat gaacgggtcg aacatctcgc tgttcaacgc
      961 cgacacgatc tactccggcg cgaacggcga tcccggcgtg ctgggcgcat ggctggcgcc
     1021 gtttggcgcg ctggcggcgc gcggtgactt ccgcgccagc gtggtgttgc tgatcatgac
     1081 cgcgatcatg gtcgcgctct gcttctggtg gctcaactcc gaacagggca tcgccctgcg
     1141 ggccaccggc gacaacccgc gcatgatccg cgcgctgggg gtcaacaccg accacatgaa
     1201 gatcctgggg ctgacggtgg cgaacgggct atgcgggttc tccggcgcgg tgctggccca
     1261 gcagttgagc tactcggata ttacgctggg gccgggcgcc atcctcacgg gcctggccgc
     1321 cgtcatcctg ggcgaggcgc tgatccacgg tagcggggtg ctggccggac tggccggcgc
     1381 cgtggtcggt gcgatcgtct tccaactgat catcgcggct acccaggtcc aacaagtctt
     1441 caccatcgag cccaccgact acaagctggc gagcgcgctc atcgtgctca tcgcactgct
     1501 ggtgccggcg gtgcgcagcc gcctgggaat gcgcagcctg gcccgcaccc ggcgcagttc
     1561 ggcggggaag gccggcgccc catgaccacc tcacccacca cggcccagcc cgcccccgcg
     1621 cccgcccccc ggcccgcggg ccgccccgcc ctggccctgc gcggcgtcgt caagaccttc
     1681 taccccggca gcgtcaacga agtccgcgcc atccggggga tcgacctgag cctccagccg
     1741 ggcgagttcg tcaccattat cggcggcaac ggcgcgggca agtccacgct gctcaacctc
     1801 attgccggcg tgttcccgcc cgacagcggg acgattaccg tcggcgggca cgagtgtacc
     1861 aacctgagcg aggaccggcg cgcgcgctgg atcgggcgcg tcttccagga cccgctggcc
     1921 ggcacggcgc ccaacctgac catcgagcag aacctggcga tggcgctgtt gcgcgggcgc
     1981 gggcgcgggc tgggccgcgg cgtgaccgac agccgccggg ccatcttccg cgagcaactg
     2041 gcccgcctgg gcatcgggct ggaaaaccgg ctgcacaccc gcgccgggct gctgtcgggc
     2101 gggcagcgcc aggccctgac cctgctcatg gcgacgttgc agaagcccga tgttctcttg
     2161 ctggacgagc acacggcggc gctggaccca gccaccgccg ccactatcgt cgatctcacc
     2221 gcccggctga tcgccgacgc gcacctgacc gcgttgatgg tcacccataa catgagtctg
     2281 gcgttgcacg tgggcacgcg cacgctgatg atgcatgagg ggcaggtcat cctcgatctg
     2341 gccggagcag agcgtcaggc ccagacggtg cgctcgctgg tcgacaggtt tttcgccctg
     2401 cggggcaccg aagtggcgtc ggaccgcatg ttgctgtact aaaaagggcc gcggggctga
     2461 tccgggttat ggctttattc gataaggagg aaccttcacg atgaacagtg cacacaccaa
     2521 aacgcgagcc gggctgccgc gcaaaggcgc cttcctcccg tggttgagct tggtgctggc
     2581 gctcgggttg ctgctctccg cctgcggccc caccccggac accggcgggc cgaccccggc
     2641 gaccggcgcc acggcgacca cggccaccgg cggcacgcag gccaccgcga cgacggcgac
     2701 cggcggcgaa gctaccgcca cctccgcccc cagcggcgaa gccacggcga ccactgccac
     2761 cggcggcgcc accgccacct ccgcccccag cggccccacc gcgacgaccg cgaccggcgg
     2821 cacgcaggcc accgccacca ccggctcggc gacgggcaag cactacaccg tcggcatctt
     2881 gcagacggcc acccaccccg ccctcgactt cacccgtgaa ggggccaagc aggcgtttat
     2941 ccaggccggc atggtcgagg gccagaccgt gaccttcgat ctgcgcaacg ccgaactcga
     3001 cgcgcccacc gccgccaaga tggccgatgc cttcatcgcc aacaaggtcg atgccatcat
     3061 caccatcggc agcctcgcct cgcaggccgc gctggccgag gagcagaagc aaggcagcac
     3121 gatcccgctc ttcttcaccg ccgtcgccga cccctatgtc gccaagctgg ccggcaagca
     3181 ggaaggtgat actattaccg ccgacccgaa gatccacccg gcctttgaga ccggcgtgca
     3241 ggccttcccg ccggtcgaag acggcctgaa gctcatcaag gaagtgaacc ccgacgtgaa
     3301 gacggtaggc ctgctctgga accccaacga gcccaactcc aaggccacca cc
//
LOCUS       DASUYC010000219         4323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_50873,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000219 DASUYC010000000
VERSION     DASUYC010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50873"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="VF276_03550"
     CDS             <1..816
                     /locus_tag="VF276_03550"
                     /inference="COORDINATES: protein motif:HMM:NF042699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6602 domain-containing protein"
                     /protein_id="HEX6605969.1"
                     /translation="MKLLHALRNAAARLAADLEDSNLFEHAGDKGEFREHIIGRFLRP
                     FLPECYGLGSGQVFAADGTSSAQVDIVVYDAIFSNVLFRDAGNSLFPCESIYGTIEVK
                     SRLSVAELETSIANGASVKRLRRAPSDGGDLLPYRRLAFAAPLALDPQQRNPYLSMIF
                     VYEGLAAPAVVETLNRHLASGRTPAALLPDYLFSYRRGFAVLRAQGNQVAPFGGPFDR
                     YVAVEAGADTLPLFFLTLNICLNSIFLRAPDFNAYWLQVFGEILRGAPGERPA"
     gene            complement(813..1277)
                     /locus_tag="VF276_03555"
     CDS             complement(813..1277)
                     /locus_tag="VF276_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605970.1"
                     /translation="MDDSTRDTIEFLIRRYALHDRLPVDLSGLLDRFTVRRYPFTAWT
                     LGFAVVRPREIHIGINAGLPVPWQRMAEAHEVGHIIAYHPHRLYTCKAYQWDQEDHER
                     TAQLAAALLLVPAAAMQTYGVEFTPAQLARLLQVPTALVDLRWTQAKLEGAI"
     gene            complement(1286..1558)
                     /locus_tag="VF276_03560"
     CDS             complement(1286..1558)
                     /locus_tag="VF276_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605971.1"
                     /translation="MIGTWVRRRRALRRRPVEPPHPDSAVAAPAPVASSPAAPAAGKA
                     PADDPRLELYLQQIADLPAHDRAIIVGVLQSILDEEAAYARRVVSE"
     gene            complement(1793..3268)
                     /locus_tag="VF276_03565"
     CDS             complement(1793..3268)
                     /locus_tag="VF276_03565"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6605972.1"
                     /translation="MDDQHPEPDSSAPPPADSALDPTTRPIAERLREGIMLLRNGDRA
                     GAYNLFRGAADTAPDNLNAWIGVALSSTSLDEAADALVRAERLDPNSRFANQAETDLT
                     RRRPGFAEALAAARERAAAAPPLAEPASSPLEAEAAPPAEPVPAEPVAGADAPVMPAA
                     ETAVISTGTRLGGQTELQPKTDDRMSRAFLTVLVGVLALAVIGVVGLGLANSPIFGPP
                     PPRPTATPTVAPTNTAAPTATVPVAQAPPTLTPLPATPTAARGPLGGTPTAVDPAVTA
                     AIAAVQAGRYADAIPALEAATERNPTDAAALYYLGVAYLNAPGRPHGAEDATLTLRTL
                     QAQHPRWAPGLDLLARSLIAQGQFRDAVPPAHQAVESDPGRPEYWTTLARAYEGAGAT
                     AEASLAYAEAAKRSGTPGATPGTLAPGSATPATGTTTPGAEVTPAGSVTPATTVTAAA
                     GTTPGAAATSTVGAVGTATATPAAGTATATATPLPAPSGIR"
     gene            complement(3285..>4323)
                     /locus_tag="VF276_03570"
     CDS             complement(3285..>4323)
                     /locus_tag="VF276_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HEX6605973.1"
                     /translation="AGACGVTSDEAEEVLRLIQRAAPTGVGARDLRECLLLQLEEIKE
                     RGRPELVPFVEEILRDHLTELGEHKFTLIAHTLGTTYDAVVAVRDFIKTHMNPHPGYA
                     ADGRPWPMPSPVRFVAPDVIIRLRNNNFEVEVVESLRFSLRISPIYHRLAAAAAAHPQ
                     GRVTTNGHGPGPEADGSGEDSGGEFSLPTVTMAGMSDNDREHIRRYVNRSRLFISNIA
                     QRRETMRRITNCLVEVQDDFLRHGVRHLRPLTRAQVAQYLGLHESTVSRATADKYVML
                     PNRQVIPFADFFQASLSAKDRIREMIVTEPKPLTDKEIVQRLREQGVRIARRTVTKYR
                     NQLGIMPSTFR"
BASE COUNT          563 a         1419 c         1656 g          685 t
ORIGIN      
        1 atgaagctac tgcacgcgtt gcgcaacgcg gcggcgcggc tggccgccga tctggaggac
       61 agcaacctct tcgagcacgc gggcgacaag ggcgagttcc gcgagcacat catcgggcgg
      121 tttctgcggc ccttcttacc ggagtgctac gggttgggct ccggccaggt tttcgccgcc
      181 gacggcacca gcagcgccca ggtcgatatc gtggtctacg acgccatctt ctccaacgtg
      241 ctcttccgcg acgccggcaa cagcctgttc ccctgcgaat cgatctacgg gaccatcgag
      301 gtcaagtcgc ggctgtcggt ggccgagttg gagacgagca tcgccaacgg cgcctcggtg
      361 aagcggctgc ggcgcgcgcc gtcggatggc ggcgatctgc tgccctaccg gcggctggcc
      421 tttgcggcgc cgctggcgct cgatccgcag cagcgcaacc cttacctgag tatgatcttc
      481 gtctacgaag gactggccgc gcccgccgtg gtcgagacac tcaaccggca cctggcaagc
      541 gggcgcacgc ccgccgcgct gctgcccgat tacctgttct cctaccggcg cgggttcgcc
      601 gtgttgcgcg cccagggcaa ccaggtggcg cccttcggcg ggccgttcga ccgctacgtc
      661 gccgtggagg cgggcgccga cacgctgccc ctgtttttcc tcactctcaa catctgcctg
      721 aattcgatct tcctgcgcgc gcccgacttc aacgcctact ggctgcaagt cttcggcgag
      781 atcctgcgcg gggcaccggg cgagcgcccg gcctaaatcg cgccctccag cttggcctgg
      841 gtccagcgca gatcgaccag tgcggtgggc acctggagca gccgggccag ttgcgcgggc
      901 gtgaactcga cgccgtaggt ctgcatggcc gcggcgggga ccagcagcag cgccgcggcg
      961 agttgcgcgg tccgctcgtg gtcctcctgg tcccactggt aggctttgca ggtgtagagc
     1021 cggtgcgggt gataggcgat gatgtggccc acctcgtgcg cctcggccat gcgctgccag
     1081 ggcacgggca gcccggcgtt gatgccgatg tggatctcgc gcgggcgcac cacggcgaag
     1141 ccgagcgtcc aggccgtgaa ggggtagcgg cgcaccgtga accgatccag caggccgctc
     1201 agatcgaccg gcagccggtc gtgcaacgca tagcggcgga tcagaaactc gatggtgtcg
     1261 cgcgtggagt cgtccatggg gtggcctact cggagacgac gcggcgggcg tacgccgcct
     1321 cttcgtcgag gatgctttgc agtacgccga cgataatggc gcggtcatgc gcggggaggt
     1381 cggcgatctg ctgcaggtag agttccagac gcgggtcgtc cgccggtgcc ttgccggcgg
     1441 caggcgcggc gggtgaggat gcgacggggg cgggggcggc tacggcagaa tccgggtgcg
     1501 gcggctcgac cgggcggcgc cggagcgcgc gccggcggcg gacccatgta ccgatcatcg
     1561 tgcgacctac gcgcctttct gccaacgcgc gtgtgagata cgcgggcgcc tcgacccgcg
     1621 gagtactcca attatagcac cgcgtcgcgg cggttcgcaa cgcgaacgtc tgtgcgtaat
     1681 gcgcgcacag cccgcccgcg acaggcgcgg catccggtag aacgaacgga gggccgcccg
     1741 gttgcccggc ggccctcatg gtgtgttgcg gagtggcctg cgcgggccgg cgtcagcgga
     1801 taccgctcgg cgcgggcagc ggggtggcgg tggcggtagc ggtgcccgct gcgggtgtgg
     1861 ccgtggccgt gccaacggcg cccaccgtgg atgtggccgc ggcgccgggc gtggtcccgg
     1921 ctgccgccgt cacggtagtg gccggcgtga cgctgcccgc gggcgtgacc tcggcgcccg
     1981 gcgtggttgt gccggtggcg ggcgtggcgc tgccgggcgc cagggtgccg ggggtggccc
     2041 ccggcgtgcc gctgcgcttg gctgcctcgg cgtaggccag gctggcctcg gcggtggcgc
     2101 cggcgccctc ataggcgcgg gccagcgtcg tccagtactc cgggcgcccc ggatcgcttt
     2161 cgaccgcctg gtgggcgggg ggcacggcgt cgcggaactg gccctgcgcg atcaggctgc
     2221 gcgccagcag atcgaggccc ggcgcccagc gcggatgctg cgcttgcagg gtgcgcaggg
     2281 tcagcgtggc gtcctcggcg ccgtgcgggc gacccggcgc gttcaagtag gcgacgccga
     2341 gatagtagag ggcggcggcg tcggtcgggt tgcgttccgt cgccgcctcc agcgctggga
     2401 tcgcgtcggc gtagcgaccc gcctgcaccg cggcaatcgc cgccgtcacc gcaggatcga
     2461 cggcggtggg ggtgccgccc agcgggccac gggcggcggt gggcgtggcg ggcaggggtg
     2521 tgagcgtcgg cggcgcctgg gccaccggca cggtcgccgt aggcgcggcg gtattagtcg
     2581 gcgccacggt gggcgtcgcg gtgggccggg gcggcggtgg gccgaagatg gggctgttgg
     2641 cgaggcccag gcccacgacg ccgatcacgg ccagcgccag cacgccgacc agcaccgtca
     2701 ggaaagcccg gctcatgcgg tcgtcggtct tgggctggag ttcggtctgc ccgcccaggc
     2761 gcgtccccgt gctgatcacc gcagtctcgg cggcaggcat gacgggcgca tcggccccgg
     2821 cgacaggttc ggcgggcact ggttcggcgg gcggcgcggc ttccgcttcc agcggggacg
     2881 atgccggttc ggcgaggggc ggggccgccg cggcacgttc gcgggcggcg gccagggcct
     2941 cggcaaagcc ggggcggcgc cgggtcaggt cggtctcggc ctggttggcg aagcggctgt
     3001 tcgggtccag gcgctcggcc cgcaccagcg catcggcggc ctcgtccagg ctggtgctgc
     3061 tgagcgcgac gccgatccag gcgttcaggt tatcgggcgc ggtatcggcg gcgccccgga
     3121 acaggttata cgcgcccgcg cggtcgccgt tgcgcaacag cataatgcct tcgcgcaggc
     3181 gttcggcgat cgggcgtgtg gtgggatcga gcgcagaatc ggcgggcggc ggggccgagc
     3241 tatcgggctc cgggtgctgg tcgtccaagg caacctcctg gcggctagcg gaaagtggat
     3301 ggcatgatgc cgagctggtt gcggtatttc gtcacagttc gccgggcgat gcgtacgccc
     3361 tgctcccgca gtcgctgcac aatttccttg tcggtgaggg gtttcggttc ggtgactatc
     3421 atctcccgga tgcggtcctt ggcgctcagg gaggcctgga agaagtcggc gaaggggatg
     3481 acctgccggt tgggcagcat cacatattta tcggcggtgg cccggctgac ggtcgattcg
     3541 tgcaggccga gatactgcgc gacctgggcg cgggtcagcg ggcgcaagtg gcgcacgccg
     3601 tggcgcaaga agtcgtcctg cacttcgacc aggcaattgg tgatccgccg catggtctcg
     3661 cggcgctggg cgatattcga gatgaacagc cgcgaacggt tgacataacg ccggatatgc
     3721 tcgcggtcgt tgtccgacat gccggccatg gtgacggtgg gcaggctgaa ctcgccgccg
     3781 ctgtcctcgc cgctgccgtc ggcttccggg ccggggccgt gcccgttggt cgtgacccgg
     3841 ccctgcggat gcgcggctgc cgccgcagcc agccggtgat agatcgggct gatgcgcagg
     3901 ctgaagcgca gcgactcgac gacctcgacc tcgaagttgt tgttgcgcaa ccggatgatc
     3961 acatcggggg cgacgaagcg caccggcgag ggcatgggcc aggggcggcc atcggcggcg
     4021 taaccgggat gcgggttcat gtgcgtcttg atgaaatcgc gcacggccac gaccgcgtca
     4081 taggtggtgc ccagcgtgtg ggcgatgagc gtgaacttgt gctcgcccag ctccgtcagg
     4141 tgatcacgca ggatctcttc gacgaagggc accagttcgg ggcggccccg ctccttgatc
     4201 tcttcgagtt gcagcaacag gcactcgcgc aggtcgcggg cgcccacccc cgtcggcgcg
     4261 gcgcgctgga tcaggcgcag cacttcctcg gcctcgtccg atgtgacgcc gcaggccccc
     4321 gcc
//
LOCUS       DASUYC010000220         4752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1898212, whole genome shotgun sequence.
ACCESSION   DASUYC010000220 DASUYC010000000
VERSION     DASUYC010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4752
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1898212"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..765)
                     /locus_tag="VF276_03575"
     CDS             complement(184..765)
                     /locus_tag="VF276_03575"
                     /inference="COORDINATES: protein motif:HMM:NF017090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="emopamil-binding family protein"
                     /protein_id="HEX6605974.1"
                     /translation="MRNPLARQAIPLAKRPGDLVLIAFFAVNLVVITYMVDVEQLIIA
                     DPARFSYPLWPPPFMVDAVHWWGRSFDPLLLARPVWWKMTIAIDSLFFGPYYVAALYA
                     FLRPRDWIWLPSLVYSGLMLANVTIILGEETWGPYRSPQLGIVFLANLPWILCPLYLI
                     YRMRTAPHPFTEATPAGETQATLPGRRPHERDR"
     gene            1002..1934
                     /locus_tag="VF276_03580"
     CDS             1002..1934
                     /locus_tag="VF276_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HEX6605975.1"
                     /translation="MDTRTLEALNAQFAIPGRIAFRSGPGGLVQAVIENPHASAEIAL
                     QGAQVLSFQPQGSAPVLFLSPHAVYAPGKAVRGGIPICWPWFGPHPTDPDKPQHGFAR
                     TLPWAVLGTEGRDDSSTEVRLGLQDTPATRALWPHPFALTLVVQVGRELHVALETHNP
                     GPESMPVTEALHAYFQIGDLAALSIGGLEGTTYIDKVAGDQRRVQSGPVTISGETDRV
                     YIDTAAACAVDDPGLQRRIEIAKEGSRSTVVWNPGPEKAAQMADLGGDEDTRMVCVEV
                     ANALDDRVVVAPGADHRLAMGIRVAPSGAPPARP"
     gene            complement(1967..2791)
                     /locus_tag="VF276_03585"
     CDS             complement(1967..2791)
                     /locus_tag="VF276_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX6605976.1"
                     /translation="MSLPEPYPDLDEFLVTLGEAGQRLAAIDAGEGAAGNMSIYIGWP
                     IEVRRVFPLVEPIDLPLPAPALAGQIVIVTGSGRRLRDVRADPAANLGAVRIGADGRT
                     GQLYTSPRRLFARLTSEFNSHLAVHDDQVARTGTNFHAVIHAQPPNLVYLTHIPRYRD
                     PQYLNRRLIRWEPETIVQLPEGVGVLPFILPGSPAMMEANVAALREYRIVLWGKHGVM
                     SRSDHSITAATDRVEYAETAARYEYMDLVNGSQAEGLTDAELREVIAAFGVETRLI"
     gene            complement(2938..3909)
                     /locus_tag="VF276_03590"
     CDS             complement(2938..3909)
                     /locus_tag="VF276_03590"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6605977.1"
                     /translation="MRLRTITDTDLHASVICLGALPFGVSLSEDLSFALMDRFVAEGG
                     NFIDTALVYGEWLPGGKGRSERTVGKWVTQRRTRDRVIIGTKGAHPRLSSMDVPRLSR
                     AEIVADLDESLGNLQTTYIDLYWLHRDDPRRPVAEILHTLNEQVQRGKIRYFGCSNWH
                     LDRVQAAQDYAAVHGIRGFAGNQLMWSLAVPNPAALFDPAMVTMDAATKAYHLRTGLA
                     AMAYSSQAGGLFTRLESGAPDDLPAGQRALYAGEENLRRLQRLQRVAQAQDLPVGVLA
                     LAYVVSHPFPAYALSGCSNMDQLQQNLRAGDVQLEPGVLEYLENGPD"
     gene            complement(3977..4360)
                     /locus_tag="VF276_03595"
     CDS             complement(3977..4360)
                     /locus_tag="VF276_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605978.1"
                     /translation="MAFYRLQAWLHRAPDQPAPPARQSVPIPEELAAILDRAATEQQR
                     LTWEALRQYAQAAGLPLELLVARAWGRRFPFAELLAAGVPQSALAGYEREWARSSPAE
                     QTRRLQEAFAYAAALRRGRHTVRER"
     gene            complement(4360..>4752)
                     /locus_tag="VF276_03600"
     CDS             complement(4360..>4752)
                     /locus_tag="VF276_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605979.1"
                     /translation="GQMVVEVVVPPNSRARVTLPGGDGSTIEVGSGSRRWSYPYQAPQ
                     PARRALTLDSSLREVIDDPAAWDATLRTIRRYMPEYNTSDIGQNGNLAMPLRGLFQFG
                     HYPNRDQVLAGIAEALRAAAPLENTEAG"
BASE COUNT          752 a         1573 c         1659 g          768 t
ORIGIN      
        1 cgcgtgctcg tccatcaagt ctgccctcgc tgatcggtcc gtggttggcg ccatcgcgga
       61 aaatgacgca cggttgcccc atttccattg tctgaatgat caacgacgcg tcttcgcggg
      121 agtgatgcgc gcggggcggc atgggctcct gggcggggtc cccgtcagcc gatcgccccc
      181 ggcttatcgg tccctctcgt gagggcgccg ccccggcagg gtcgcctggg tttcgccggc
      241 gggggtcgcc tcggtgaagg ggtgtggcgc agtccgcatg cggtagatca gatacagcgg
      301 gcagaggatc cagggcaagt tcgccaggaa cacgatgccg agttgcggcg agcggtatgg
      361 cccccaggtc tcttcgccga ggatgatggt gacgttggcc agcatcaagc cgctatagac
      421 gagactgggc agccagatcc agtcgcgggg gcgcagaaag gcgtacagcg ccgctacgta
      481 gtacggcccg aagaacaacg agtcgatcgc gatggtcatt ttccaccaga cgggtcgggc
      541 cagcagcagc ggatcgaagc tgcggcccca ccagtggacg gcatcgacca tgaacggtgg
      601 cggccagagc gggtaactga agcgggccgg gtcggcgata atgagttgtt cgacatcgac
      661 catgtaggtg atgacgacca ggttcaccgc gaagaaggcg atcaggacaa ggtcgcccgg
      721 tcgtttggct aagggaatcg cctgccgggc aagtggatta cgcatggtgg tggccgatct
      781 gggtgtcagg ctgagccgtc ctggttgctg cgtcctgtgt gcctcattgg gatcagtata
      841 ccgtagaata cctgagaggt tctatagata cctgttattg atccgcccgg taggccgcgg
      901 gcggcggccc gctggcgcgg atcttgccgc catgcgacac cgcaccggcc gccgcaggaa
      961 gacggcccct tgagttcacg ggccggcgca aggaggcagg catggacacg aggaccctgg
     1021 aagccctgaa cgcgcagttt gccatccccg gccggatcgc gtttcgatcc ggccccggcg
     1081 ggctggtgca ggccgtgatc gaaaatccgc acgccagcgc ggagatcgcg ctgcagggcg
     1141 cccaggtgct gtccttccag ccgcagggca gcgcgcccgt gctcttcctg agtccgcacg
     1201 ccgtctatgc gccgggcaaa gccgtccgag gcgggatccc gatttgctgg ccgtggtttg
     1261 gcccgcaccc caccgacccg gacaaacccc agcacggctt cgcccgcacc ctgccctggg
     1321 cagtgctcgg gacggaaggg cgggacgaca gcagcaccga agtgcgcctg ggcctgcaag
     1381 acacgccggc cacccgcgcg ctgtggccgc atcccttcgc gctgaccctg gtggtgcagg
     1441 ttggacgaga gttgcacgtc gcgctggaga cgcacaatcc ggggccggag tccatgcccg
     1501 tgaccgaggc gttgcatgcc tattttcaga tcggcgatct tgcggcgctg agcatcgggg
     1561 ggctggaagg caccacctat atcgacaaag tcgccggcga tcagcgccgg gtgcagagcg
     1621 ggccggtcac gatctccggc gaaacggacc gggtctatat cgatacggcg gcggcgtgcg
     1681 ctgtcgacga tccggggttg cagcggcgca tcgagatcgc gaaggagggc agccgctcta
     1741 ccgtggtctg gaatccgggg ccggagaaag ccgcgcagat ggccgacctg ggcggcgacg
     1801 aggatacgcg gatggtctgc gtcgaggtgg cgaatgcgct tgatgatcgc gtcgtcgtcg
     1861 cgccgggcgc tgatcaccgg ctggcgatgg gcatccgcgt ggcgccgtca ggggcgccgc
     1921 ccgcccggcc ctagcgctga cgcgatcctg cccgcccact gcggacctag atcaggcggg
     1981 tctccacgcc gaaggccgca atcacctcgc gcaactcggc atcggtcagg ccctcggcct
     2041 ggctgccgtt caccaggtcc atgtactcgt agcgggcggc ggtctcagcg tactcgacgc
     2101 gatcggtcgc cgccgtgatc gagtggtccg agcgcgacat caccccatgt ttgccccaaa
     2161 gcacgatgcg gtactcgcgc agcgcggcca cgttcgcctc catcatcgcc ggggagccgg
     2221 gcaggatgaa cggcagcacg ccgactcctt ccggcaactg cacgatcgtt tccggctccc
     2281 agcgaatcag ccgccggttc aaatactgcg ggtcacgata gcggggaatg tgcgtgaggt
     2341 agaccaggtt gggcggctgc gcgtggatca cggcgtgaaa gttggtgccc gtgcgggcga
     2401 cctggtcgtc atgcacggcc aggtgcgagt tgaattcgct ggtcagccgg gcgaacaggc
     2461 ggcgcggtga ggtgtagagc tgcccggtcc gcccatccgc gccgatgcgt acggcgccaa
     2521 ggttagccgc cggatcggcg cgcacatcgc gcaggcgccg gcccgagccg gtgacgatca
     2581 cgatctgtcc tgccaacgcc ggggccggca agggaagatc gatcggctcg accagcggga
     2641 agacgcggcg cacctcaatg ggccagccga tatagatcga catattgccg gcggcgcctt
     2701 ccccggcgtc gatggccgcc aggcgctggc ccgcctcgcc gagagtcacc aggaattcgt
     2761 ccaggtcggg atacggttcc ggcagcgaca tgcttgcctc ctcctctaac tatccgcctc
     2821 cgcgcgggaa cgccctgtgc aacgccccgg cgaccgcggt gatccggtcc gggtgtgccg
     2881 tcgctcggcc ggcaggacgt tgcgccgctc ggcccttgcg catcgcgccg ccgggcgcta
     2941 gtccggtccg ttttcgaggt actccagcac acccggctcc aattgaacat caccggcgcg
     3001 caggttctgc tgcaattggt ccatattcga gcaacccgat agcgcgtacg ccgggaacgg
     3061 gtgtgacacc acataggcca gtgcaagtac gccgaccggc agatcctggg cttgcgcgac
     3121 ccgctgcaaa cgctgcagcc gccgcaggtt ctcttcaccg gcgtacaggg cgcgctggcc
     3181 cgcgggcaga tcgtccggcg ctccggactc cagccgggtg aacaggccgc cggcttgcga
     3241 actgtaggcc atggcggcca accccgtgcg caagtggtag gccttcgtcg ccgcatccat
     3301 tgtcaccatg gccggatcga acagcgcggc ggggttgggc accgccaggc tccacatcag
     3361 ttgattgccg gcgaacccgc gaatcccgtg caccgcggca tagtcctggg ccgcctggac
     3421 gcgatccagg tgccagtttg agcacccgaa atagcggatc ttgccgcgct gcacctgttc
     3481 attcagcgtg tgcagaatct cggcgaccgg gcggcgcgga tcgtcccgat gcagccagta
     3541 gagatcgatg taggtggttt gtaagttgcc caggctttcg tccaggtcgg ccacgatctc
     3601 ggcgcgcgac agccgcggca catccatgct ggacagccgg ggatgggcgc ctttcgtgcc
     3661 gatgatgacc cggtcgcggg tgcgccgctg cgtgacccac ttgcccacgg tccgctcgct
     3721 ccggcccttg ccgccgggga gccactcccc gtagacgagc gccgtgtcga tgaagttgcc
     3781 gccctcggcc acgaagcggt ccatcagggc gaaggacaga tcctcgctca ggctcacgcc
     3841 gaacggcaac gcccccaggc agatgaccga ggcgtggagg tcggtatcgg tgatggtgcg
     3901 cagtctcatc gtgcttcccc gcgctgcttg cttgcggtcc ggcagagatc ggtcaggtgc
     3961 cgggtcgaca cgcgccctac cgctcccgca cggtgtggcg gcctcgccgc aaagccgcgg
     4021 cataggcgaa tgcctcttgc agccgccggg tctgctcggc cggcgaactg cgggcccact
     4081 cccgttcgta gcccgccagc gcgctctggg gcacgcccgc ggccagcagc tccgcgaacg
     4141 gaaagcgccg gccccaggcg cgggccacca gcaactccag cggcaagccg gcggcctggg
     4201 catactgccg cagcgcttcc caggtcaggc gctgttgttc cgtcgcggcg cggtccagaa
     4261 tggcggccag ttcctcgggt atgggcacgc tctgccgggc cggcggcgcg ggctggtcgg
     4321 gcgcccggtg caaccaggct tgcaatctat agaacgccat cagccggcct cggtgttttc
     4381 tagaggggct gccgcccgca gcgcttccgc gatgccagcc agcacctggt cccggttggg
     4441 atagtgcccg aactggaaca gcccgcgcaa gggcatggcg aggttgccgt tctggccgat
     4501 gtcgctggtg ttgtactccg gcatgtagcg gcggatggtg cgcagggtgg cgtcccaggc
     4561 ggcggggtca tcgatcactt cgcgcaggct gctgtccagg gtcagcgcgc gccgggccgg
     4621 ctgcggtgcc tggtagggat aggaccagcg ccggctgccg gacccgacct cgattgtgct
     4681 gccgtcgccg ccggggagcg tcacgcgggc cctggagttg ggtgggacca cgacttcgac
     4741 caccatctgg cc
//
LOCUS       DASUYC010000221         2420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_560629,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000221 DASUYC010000000
VERSION     DASUYC010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560629"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VF276_03605"
     CDS             <1..511
                     /locus_tag="VF276_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX6605980.1"
                     /translation="TSGGMFVDFFVHDIDLARFLVGEVEEVNAWGSVLIDPGFVDAGD
                     VDTAVAMLRFRNGALGVLEGSRRCTWGYDIRTEVAGSRAKLVIEAPRKTPLQTMRDLG
                     VAEDHYESFPDRFHAAYRAELEYFFACLRAGTPPAPGVRDALETLRVALAAAKSMAEG
                     RPVKVDEIQ"
     gene            526..1419
                     /locus_tag="VF276_03610"
     CDS             526..1419
                     /locus_tag="VF276_03610"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX6605981.1"
                     /translation="MKAASAPVSWGITEIEGLQADLNYGQVMDEIATAGYEGTELGPW
                     GFFPTAPDGLRGALGRRGLSLVGAFVDVPIHDPAQFGVGRQAVRQVVPLLAALGAPTL
                     ILSARQTPERAAIPGRVTEADGLSPAQWRQAGAYLQELAGIARDAGLDATFHHHVGTY
                     VETPQEIARLFDAVDPALLGLCLDTGHLVFGGGDNAAFLARYAPTVRHLHLKNVDAAV
                     LDQVRSGDMNYLQAIRTGVFAPLQAGSIDIPAVIATLQRAGYDGWAVVEQDIDRSQPD
                     YPDPLQGATAARRFLREAAGI"
     gene            1524..2420
                     /locus_tag="VF276_03615"
     CDS             1524..2420
                     /locus_tag="VF276_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6605982.1"
                     /translation="MNYGVTLPPFDDFFEPRTLAEIAREAEEAGWDGFFLWDHVLLWP
                     TPIADPWVALAAVALATERIRIGPLVTPLPRRRPTKLARETVSLDHLSRGRLVLGVGS
                     GGGPWEWDYLGEVRDPRVRGAMLDEGLELLAKLWSGEPVLHEGRFYQFRGDLGPDNPA
                     VSPTPFLPRPVQSPRIPVWVAGTWPRKAPFRRAARWDGVVPLAEGKAFGEYMTPEEIR
                     QITAYVRGQRTSAEPFDVVVSGHTAGTDPAADSALVQEYAGAGATWWLEDISPWPFGW
                     TWQGPWPTAAMRARIRQGPPRD"
BASE COUNT          357 a          874 c          838 g          351 t
ORIGIN      
        1 caccagcggc ggcatgttcg tcgacttctt cgtgcatgac atcgacctgg cccggttcct
       61 cgtgggcgag gtcgaggaag tcaacgcctg gggcagcgtg ctgatcgatc ccggctttgt
      121 cgacgcgggc gacgtggaca ccgccgtggc gatgctgcgc ttccgcaacg gcgcgctcgg
      181 cgtgctcgaa ggctcgcggc gctgcacctg gggctatgat atccgcaccg aggtggccgg
      241 cagccgcgcc aagctggtga tcgaggcgcc gcgcaaaacc ccgttgcaga ccatgcgcga
      301 cctcggcgtc gccgaagacc actacgagag cttcccggat cgcttccacg cggcgtaccg
      361 cgccgagttg gaatacttct tcgcctgcct gcgggcgggg acgccgccgg ctcccggcgt
      421 gcgcgatgcg ctcgaaaccc tgcgcgtggc cctggccgcg gcaaaaagca tggccgaggg
      481 ccggcccgtg aaagtcgacg aaatccaata ggaggaaagc gtttcatgaa agcagccagc
      541 gcgcccgtct cgtggggcat caccgagatc gagggcctgc aagcggacct gaactacggc
      601 caggtcatgg acgagatcgc aacggcggga tacgagggca ccgaactggg gccgtggggc
      661 ttctttccga cggcgcccga tggcctgcgg ggcgcgctgg gccggcgcgg tctgtcgctg
      721 gtgggcgcgt tcgtcgacgt acccatccac gacccggcgc agttcggggt gggccgccag
      781 gcggtgcgcc aggtggtgcc gctgctggcc gccctgggcg cccccacgct gatcctctcg
      841 gcccggcaga cccccgagcg ggcggcgatt ccgggccgcg tgaccgaagc cgacgggctc
      901 agccccgcac aatggcggca ggccggagcg tatctccagg aactggccgg catcgcccgc
      961 gacgccgggc tcgatgccac tttccaccac cacgtcggca cctacgtcga aaccccgcaa
     1021 gagatcgcgc gcctgtttga cgcagtggac ccggccctgc tggggctttg cctcgacacc
     1081 ggccatctcg tgttcggcgg gggcgacaac gcggcctttc tggcccgtta cgcgcccacc
     1141 gtccggcatc tgcacctgaa gaatgtggac gccgccgtgc tcgaccaggt ccgcagcggc
     1201 gacatgaact acctgcaagc gatccgcacc ggggtgttcg cgccgctgca agcgggcagc
     1261 atcgacattc ccgccgtgat cgccaccctg caacgcgccg gatacgacgg ctgggcggtg
     1321 gtcgagcagg acatcgaccg cagccaaccc gactatcccg accccttgca gggcgcgaca
     1381 gccgcccggc gcttcctgcg cgaagccgcc ggcatataag ccgccgctgg ggcacccggc
     1441 gcgccccacg cagcgtgggc gcgccgcggt gccgatttcg cttgctacac tccccggccc
     1501 atgcaacgga gggactttga ccattgaact acggcgtgac cctaccgccc tttgacgact
     1561 tcttcgagcc gcggacgctg gccgagatcg cgcgcgaggc cgaagaggcc ggctgggacg
     1621 gattcttcct gtgggaccat gtgctgctct ggcccacgcc tatcgccgac ccctgggtgg
     1681 cgctggccgc cgtggccctc gccaccgagc gcatccgcat cggcccgctg gtcacgccgc
     1741 taccgcggcg ccggcccacc aagctggccc gcgagacggt ctccctggac cacctctcgc
     1801 ggggccggtt ggtgctgggt gttgggtcgg gcggcggccc ctgggagtgg gactacctgg
     1861 gcgaggtgcg cgatccgcgc gtgcgcggcg ccatgctgga cgagggactc gaactgctgg
     1921 caaagctgtg gagcggcgag ccggtgctgc acgaagggcg cttctaccag ttccgcggcg
     1981 atctcggccc cgataatccg gccgtctccc ccacgccgtt cctgccgcgg cccgtccagt
     2041 ccccgcgcat ccccgtctgg gtggcgggca cctggccccg aaaagcgcct ttccgccggg
     2101 ccgcccgctg ggacggcgtg gtgccgctcg cggaaggaaa agcctttggc gagtacatga
     2161 ccccggagga gatccgccag atcaccgcct acgtgcgcgg gcagcgcacg agcgccgagc
     2221 cgtttgacgt cgtggtcagc ggccacaccg ccgggaccga cccggcagcc gacagtgcgc
     2281 tggtccagga atatgccgga gccggcgcga cgtggtggct cgaagacatc agtccctggc
     2341 cgttcggctg gacctggcaa ggcccctggc ccaccgcggc gatgcgcgcc cgcatccggc
     2401 aaggcccgcc ccgcgactga
//
LOCUS       DASUYC010000222         3050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_884051,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000222 DASUYC010000000
VERSION     DASUYC010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884051"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="VF276_03620"
     CDS             <1..1049
                     /locus_tag="VF276_03620"
                     /inference="COORDINATES: protein motif:HMM:NF013254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HEX6605983.1"
                     /translation="GTVVAHTFAEAREALLLMEQAGILREGEGLETVVCEEFLEGREV
                     SALAFTDGKVVVPMPSACDYKRLLDGGIGPMTGGMGGYSPAPWISDEQWAEIAATVLL
                     PTVAGMAEEGIPFQGFLYAGLMMTAQGPRVLEYNVRLGDPEAQLLLPRLESDLVDILL
                     AITEGRLHAVAPGIVWSPEATCGVVLASGGYPAQSLIGRPISGLGELPDGVLVFHAGT
                     RVQQEGPVWVRSKDPSRPPPPDLYDSPMDETPALAAKGIYRYLRLKALPSTPGKSSLE
                     AQMDALLAGPRMVTAGGRVLTLVARAPTLDEAHRRVYAAAERVTFEGRQYRKDIGLLT
                     EPTPAKQIPGSTAQ"
     gene            complement(1120..1851)
                     /locus_tag="VF276_03625"
     CDS             complement(1120..1851)
                     /locus_tag="VF276_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX6605984.1"
                     /translation="MSRLVLFIDDGGVMNDNERRGAQWRPLVGQFFAVRLGGTPEAWA
                     AANYTVMMRMLETAAWQARMEAVPDYAEFDRLYLRDWLQGMCEIVGVPAPPEDEGRDM
                     ARAATAGIIPQINSAYPGVVDAIRALHTQGYTLHTASGEASSDLEGYLTGMGVRDCFG
                     TLYGTDVVSTFKMGTQFYTRIFAHAGVAPQQALVADDDPQAVAWAAQVGAQAVLVRAT
                     GEAGSWTGPRIDRLAELPDWLAHQS"
     gene            complement(1953..2528)
                     /locus_tag="VF276_03630"
     CDS             complement(1953..2528)
                     /locus_tag="VF276_03630"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX6605985.1"
                     /translation="MSNHTPEEAQAQIQVLDTTRVYDGKIFKVDVEDVRLPSGRTSKR
                     EIVRHPGAVALMVIDPDGKLLLVRQYRRATDRILLEVPAGTREPGEDAEACARREVQE
                     ETGFAAGTVEHLGGFYSAPGFCTEYLECYLCTDLRPGDLQAEEDEDLQLERMSLDEAW
                     AAAAAGTITDAKTLAGLLLYAARHPEARPRG"
     gene            complement(2600..2671)
                     /locus_tag="VF276_03635"
     tRNA            complement(2600..2671)
                     /locus_tag="VF276_03635"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2637..2639),aa:Cys,seq:gca)
     gene            complement(2842..>3050)
                     /locus_tag="VF276_03640"
     CDS             complement(2842..>3050)
                     /locus_tag="VF276_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HEX6605986.1"
                     /translation="AGTHIDLRHLAPTAIGRPVVAHARLTGRRGRRSTFACRVLSGDT
                     LIAEGTYEHAIIDQARILATASEG"
BASE COUNT          464 a         1011 c         1089 g          486 t
ORIGIN      
        1 agggcaccgt cgtggcccac accttcgccg aggcgcgcga ggcgctgctg ctgatggagc
       61 aggccgggat cttgcgcgag ggcgagggcc tggagaccgt ggtctgcgag gagtttttgg
      121 aggggcgtga agtcagcgcg ctggccttca ccgacggcaa agtggtcgtg ccgatgccct
      181 cggcgtgcga ctacaagcgc ctgctcgacg gcggcatcgg cccgatgacc ggcggcatgg
      241 gcggctacag cccggcgccc tggatcagcg acgagcagtg ggcggagatc gcggccacag
      301 tgctcttgcc cacggtggcg ggcatggccg aggaaggcat ccccttccag ggcttcctct
      361 acgccgggct gatgatgacg gcgcaggggc cgcgcgtgct cgaatacaac gtgcgcctgg
      421 gcgacccgga ggcccagttg ctgttgccgc gcctcgaaag cgacctggtg gacatcctgc
      481 tggcgataac cgagggccgg ctgcacgcgg tggcgcccgg catcgtctgg agtccggagg
      541 ccacctgcgg cgtggtgctg gcctccggcg gctacccggc gcagagcctg atcgggcgcc
      601 cgatcagcgg cctgggcgag ttgccggatg gcgtgctggt tttccatgcg ggtacacggg
      661 tgcagcaaga ggggccggtc tgggtgcggt ccaaggatcc cagccgccca ccgccccccg
      721 acctctacga ctcgccgatg gacgagacgc ctgcgctagc cgccaagggc atctaccgct
      781 acctgcggct caaggcgctg ccgagcacgc cgggcaagag ttcgctggag gcgcagatgg
      841 atgcgctgct ggccgggccg cgcatggtga cggcgggcgg acgggtgctg accctggtgg
      901 cccgcgcgcc cacgctggac gaagcgcacc gccgggtcta tgccgctgcc gagcgcgtca
      961 cgttcgaggg ccggcaatac cgcaaggaca tcggcctgct gaccgagcct accccggcca
     1021 agcaaattcc cggtagtaca gcgcagtagt tcgccgagcg tggccgggcc ggtttgaaac
     1081 cggcccctac acagccggtg gggtgggaaa ccgctgtgtt cagctctggt gcgcgagcca
     1141 gtcgggtaac tcggccaggc ggtcgatgcg cgggccggtc caggaaccgg cctctccggt
     1201 ggcgcgcacc agcacagcct gggcgcccac ctgtgcggcc caggcgacag cctgcgggtc
     1261 atcgtcggcc accagcgcct gctgtggcgc gacacccgcg tgcgcgaaga tgcgggtgta
     1321 gaattgcgtc cccatcttga acgtactgac cacgtccgtg ccgtagagcg tgccgaaaca
     1381 gtcgcgcacg cccatgcccg ttagataacc ttccaggtcg ctggaggcct cgccggaggc
     1441 ggtgtgcagc gtgtagccct gggtgtgcag cgcgcggatg gcgtccacga cgccggggta
     1501 ggccgagttg atctgtggga tgatcccggc ggtggccgcg cgggccatgt cccggccctc
     1561 gtcctccggg ggcgcgggca cacccacgat ctcgcacatg ccctgtagcc agtcgcgcag
     1621 atagaggcgg tcgaactcgg catagtcggg caccgcctcc atacgcgcct gccaggcggc
     1681 ggtttccagc atgcgcatca tcacggtata gtttgcggcg gcccaggctt cgggagtgcc
     1741 gcccaggcgg accgcgaaga actggccgac caggggccgc cattgggcgc cgcggcgctc
     1801 gttgtcgttc atcacgccgc cgtcgtcgat aaacaataca agccgggaca cgccggacta
     1861 tctccttcgc tatacaggcg cataatgcgc cccgctcctg tccgcccggt cctggcgccg
     1921 gagagacggc gccaggacca tggggggtac aatcagccgc ggggccgggc ctccgggtgg
     1981 cgggcggcgt agagcagcag gcccgccagc gtcttggcgt cggtgatcgt gcccgcggcg
     2041 gcggcggccc aggcttcatc caggctcatg cgttctagct gcaggtcttc gtcctcctcc
     2101 gcctggagat cgccggggcg cagatcggtg cagaggtagc actccaggta ctcggtgcag
     2161 aagccgggcg cggagtagaa gccgcccagg tgctcgacgg tgcccgcggc gaagccggtt
     2221 tcctcttgca cttcgcggcg cgcgcaggct tcggcgtctt cgccgggttc gcgggtgccc
     2281 gcgggcacct ccagcaagat ccggtcggtg gcccggcgat actggcgcac cagcagcagc
     2341 ttgccgtccg gatcgatcac catcagggcg acggcgccag ggtggcgcac gatctcgcgc
     2401 ttgctggtgc gcccgctcgg cagccgcaca tcctccacat ccaccttgaa gatcttgccg
     2461 tcgtaaacgc gcgtcgtgtc gaggacctgg atctgggctt gcgcctcttc gggcgtgtgg
     2521 ttgctcatgg gggtctcctc cggggccggt caatacgaaa ggcactaccc aggggcagtg
     2581 ccttcacagg tcagacggga ggcgacgagc ggattcgaac cgctgcatgg aggttttgca
     2641 gacctctgcc ttaccacttg gctacgtcgc cacaacgctg tatgcgggac taatatacca
     2701 gatcggcccg cctctgtcaa ccacggacga gtggtcagtg gtcggtggtc agtggtcagt
     2761 agagacgagt aacctaccgc gctacgcaac gctaagccag tagcccacat cacttgtctt
     2821 actgatcact gaccactcgt ctcagccctc gctcgcggta gcgaggatgc gggcctgatc
     2881 gatgatcgcg tgttcgtagg tgccctcggc gatcagggtg tcgccactga ggacgcggca
     2941 ggcgaaggtg ctgcgccggc cccggcgccc agtcaggcgg gcgtgggcca cgaccgggcg
     3001 gccaatcgcc gtgggggcca ggtggcgcag gtcgatgtgc gtcccggcca
//
LOCUS       DASUYC010000223         2463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1203951, whole genome shotgun sequence.
ACCESSION   DASUYC010000223 DASUYC010000000
VERSION     DASUYC010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1203951"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /gene="hemB"
                     /locus_tag="VF276_03645"
     CDS             complement(<1..706)
                     /gene="hemB"
                     /locus_tag="VF276_03645"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX6605987.1"
                     /translation="MTLTPIRETPQETGTPVPARRPRRLRRSGTLRNMVRETTLDAAD
                     FIYPLFVAEGQGFERPIGSMPGQAQRSVDLLAPEVDDLAALGIPAVLLFGIPAHKDAT
                     GSAAWDPQGPVARAAAELKRRNPDLLVIADVCMCEYTDHGHCGLLDGEQGDVLNDETL
                     ALLGRAAVAYADAGVDIVAPSAMMDGQVAAIRAALDGAGHRDVPILAYAAKFASAFYG
                     PFREAAESTPRFGDRRS"
     gene            complement(711..>2463)
                     /gene="hemC"
                     /locus_tag="VF276_03650"
     CDS             complement(711..>2463)
                     /gene="hemC"
                     /locus_tag="VF276_03650"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX6605988.1"
                     /translation="DNVMQLTIGTRGSKLALVQAEQVRAALLARHPGLAVTLEIITTR
                     GDVVLDRPLTAFSDKGLFVDEIEAALRAGRIQMAVHSAKDLPSELAADMTIAAFLPRA
                     DPRDVLVSDSGARLRDLPPGARVGTSSLRRACQLRAARPDLDVRDIRGNVDTRLRKLH
                     EGQYDAIVLAAAGLQRIDRADTITEYLDPAVMLPAVGQGALAVEICRGDEATAAFIGA
                     LDDPATRHAVQAERAFLARLGGGCKLPAAGYATVAGPHLHLAGLVGAADGRLVRDAIA
                     GDVADGARLGETLAGRLLASGGAALLDEARTDSPPGAAMVPGPLAGRRIVVTRAQEQS
                     GPLLAELRALGAEALECPAIAIAPPADYAPLDAALRNLAGYDWLLFTSANGVRAAFDR
                     MRDLGLGGGNPPAIRLPAGLRVGAIGPATAAALAEYGVQADFVPDTYVAEAVVAQIDD
                     MAGQRILLPRADIARPALAEGLRAKGALVTQVDAYRTVPGAGNPRALALLAAGQVDAV
                     TFTSSSTVRFFLNMLAEAGLDRAAARDALSRAQVVCIGPITAATAGECGIPVTAVADE
                     YTAAGVVAALRALLEKR"
BASE COUNT          349 a          880 c          910 g          324 t
ORIGIN      
        1 aactgcgccg gtcgccgaag cgcggagtcg actcggcggc ctcgcggaac gggccgtaga
       61 aggcggaggc gaacttggcg gcgtaggcca ggatcggcac atcccggtgc ccggccccgt
      121 caagcgcggc gcggatcgcg gccacctgcc cgtccatcat cgcgctgggc gccacgatgt
      181 ccacgcccgc atcggcgtag gcaaccgccg cccggcccag cagggccagc gtctcgtcgt
      241 tcagcacgtc gccctgctcg ccgtcgagca ggccgcagtg gccgtggtcg gtgtactcgc
      301 acatgcacac gtcggcgatc accagcagat cggggttgcg gcgcttcagt tcggcggcgg
      361 cgcgcgccac ggggccttgc gggtcccagg ccgcgctgcc ggtggcgtcc ttgtgcgcgg
      421 gaatgccgaa cagcagcaca gcggggatac caagcgcggc gaggtcgtcc acctccggcg
      481 cgagcaggtc caccgagcgc tgcgcctggc ccggcatcga gccgatgggc cgctcgaagc
      541 cctggccctc ggcgacgaag agcgggtaaa tgaaatcggc ggcatccagc gtggtttcgc
      601 gcaccatgtt gcgcagggtg ccgctgcggc gcaggcggcg tgggcggcgg gcgggcaccg
      661 gcgtccccgt ttcttgcgga gtctcgcgga tgggagtcag ggtcatcgat ctacctcttc
      721 tctaagagtg cgcgcagggc ggccacgacc ccggcggcgg tatattcgtc cgcaacggcg
      781 gtcaccggga tgccgcattc ccccgcggta gcggcggtta tcgggccgat acaaactact
      841 tgcgcgcggg atagggcgtc gcgggccgcg gcccggtcca ggcccgcttc ggccagcata
      901 ttcaggaaaa agcgcaccgt ggaggaactg gtgaaggtga cggcgtccac ttgcccggcg
      961 gccagcagag cgagcgcccg cggattgccc gcgccgggca ccgtgcggta ggcatcaacc
     1021 tgagtcacga gcgcgccttt cgcgcgcaaa ccctcggcca gcgccgggcg ggcgatgtcg
     1081 gcgcgcggca gcaagatgcg ctgcccggcc atgtcgtcga tctgggcgac gacggcttcg
     1141 gccacatagg tatcgggcac gaagtcggcc tgtacgccgt actcggccag cgccgccgcc
     1201 gtcgccgggc caatggcgcc cacgcgcaga ccggctggca gccggatcgc gggcggattg
     1261 ccgccgccga ggcccaggtc gcgcatccgg tcgaaggcgg cgcgcacgcc gttggcgctg
     1321 gtgaacagca gccagtcgta gccggccagg ttgcgtagag ccgcatccag tggggcataa
     1381 tccgccgggg gcgcgatggc gatggccggg cactccaggg cctcggcgcc cagcgcgcgc
     1441 aactcggcca gcaacggccc gctctgttcc tgcgcccgcg tgaccacgat gcggcgcccg
     1501 gccagcggtc ccggcaccat cgcagcgccg ggtggcgaat ccgtccgcgc ttcgtccagc
     1561 aacgccgcgc cgccgctcgc cagcaggcgc ccggccaggg tctcgcccag gcgcgcgccg
     1621 tccgccacat ccccggcaat cgcatcgcgc accaggcgcc cgtcggcggc gccaaccagc
     1681 ccggccaggt gcaggtgcgg ccccgccacg gtcgcatacc cggcggcggg caacttgcag
     1741 ccgccgccca gccgggccag gaaagcgcgc tcggcctgca cggcgtggcg ggtagccggg
     1801 tcgtccaggg cgccgataaa ggcggcggtc gcctcgtcgc cccggcagat ctcgacggcc
     1861 agcgcgccct gccccaccgc gggcagcatg accgctgggt ccaggtactc ggtgatcgtg
     1921 tcggcccggt cgatgcgctg gaggcccgcg gcggccagca cgatggcgtc gtactgccct
     1981 tcgtgcagtt tgcgcaggcg ggtatcgacg ttgccgcgga tatcacgcac atccaggtcg
     2041 ggccgggcgg cgcgcaactg gcaggcgcgg cgcaggctgc tggtgcctac ccgcgcgccg
     2101 ggcggcaggt cgcgcagccg ggcgccggag tccgagacga gcacatcacg ggggtcggcg
     2161 cggggcagga acgcggcgat ggtcatatcg gcggccagtt cggagggcaa gtccttggcg
     2221 ctgtgcacag ccatttggat gcggcccgcg cgcaaggcgg cctcgatctc gtcgacgaac
     2281 aggcccttgt cgctgaaggc ggtcaggggc cggtccagca ccacatcgcc gcgcgtggta
     2341 atgatttcga gagtcacggc cagccccgga tgccgggcca gcagggcggc ccgcacttgc
     2401 tcggcctgca ccagggccag cttgctgccg cgcgtgccga tggtcagttg cataacgttg
     2461 tcc
//
LOCUS       DASUYC010000224         5102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1993365, whole genome shotgun sequence.
ACCESSION   DASUYC010000224 DASUYC010000000
VERSION     DASUYC010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5102
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1993365"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1650
                     /locus_tag="VF276_03655"
     CDS             <1..1650
                     /locus_tag="VF276_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605989.1"
                     /translation="QLLRRQRVLLVVDNFETVTDRSLLPWLLRLPEPSKALVTSREYS
                     RAFRNNTFVLDLRGMSETEAQELIAQRLRMLRMDALVRDPAELAPLVAATGGNPKAIE
                     ISLGCLKYEHRPFRQVIDDLYAARGELFEDLFSRCWALLDEAARRVLLAMPLFLDSAG
                     RPALAATADVQGFAFDRAVERLTDLALLDAHQPDLHSAPRYTLHPLVRAFAASRLREQ
                     PDCEAAARERWMDWYRCLVAQVGYCWDDLTRLELLDAEQDTVHAVAQWTFEHHRYADT
                     LAMARGAGYYYYVRALWDKQPHINLLRAEAARHLGDLTGEIEALAYHVHMLSRQGNVA
                     EAETYLGRLDGMAAGVPLPPEVLYEYQYATALYWMARGHLDTAEAACRRSLGLAEHLT
                     PNQYVIARQWLATCLYQQGKLPEGAALFAESLLDATRLGLQRCIIFGMVRLAAIDLDQ
                     GRIDAALAALGDSQARAQQYQDRDNLAQIARLYARAHALRGDLAAARGALTTALDLFE
                     RLGMRRELAEARAELARLAAPDAAAPNAAPGLPQPPAAARR"
     gene            1790..2824
                     /gene="add"
                     /locus_tag="VF276_03660"
     CDS             1790..2824
                     /gene="add"
                     /locus_tag="VF276_03660"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEX6605990.1"
                     /translation="MTTLDPDLDALIRAMPKAELHVHLEGSVRPATLLTLARRHGVDL
                     GCQDEAGLRELYRFRDFRHFIDLYKVISEAMRAPEDFVLIISELGRDAAAQGTRYVET
                     TFSIAPHVRNKGLPADEILDAIGAAADATRRETGVEIRFILDHVRMAPPDECRQVAEW
                     CVAGRDRGVVALGLGGYEPERPASLFAESIRWAQAQGVPFVPHAGEAVGPEGVWDALQ
                     FDPPRIGHGFRAAEDPALVAVLRDRSVVLEICPTSNVCTASVPSLAAHPLRTLWDAGV
                     PISLNSDDPPMFNTTLVDEYRVAATHFGFTPADLAQVSRTAIGAALLPPAERARLVAE
                     FDAELRRLGL"
     gene            2840..3538
                     /locus_tag="VF276_03665"
     CDS             2840..3538
                     /locus_tag="VF276_03665"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEX6605991.1"
                     /translation="MHLHLFSGGGLAMTATVVARAAERLAGVPHPVIGWIPAASVDPP
                     GAWIDYMTQAFAPVGTIRLIDLDLADAASFAADIAACAAVFVPGGNTYLLSKRMHRHR
                     AMGTLSDAVRGGVPLLTRSAGTVFCGPNMLTTNDWNVFGTTEFDALGLSPYNFNVHFT
                     LPPEDEPESRDFRIRQYIAVNRRPVLALEETCNLEWTDSAVRVHGATCWLYTTSGERR
                     PLPPGSTFSPSLGG"
     gene            3540..4364
                     /locus_tag="VF276_03670"
     CDS             3540..4364
                     /locus_tag="VF276_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012187891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6605992.1"
                     /translation="MQALSMSILIRPAPKPDRHIPYGPDPQQFGHLRLPAGPGPHPLA
                     VVIHGGFWRNAYDLKHIGHLCAALGRAGLATWSLEYRRLGDAGGGWPGTFQDVARGTD
                     HVRALAAEYPLDPARVITVGHSAGGHLACWLAGRHRIPPRDILHSPDPLPLAGAVALA
                     GVLDLARAWNLRLSKGVVCEFMGGAAEEMPERYATASPIELLPLGVPQWLVHGTEDGN
                     VPYEISETYHAAALAAGDDSHLILLPGAGHFEVIDPRSREWPQVQEAILSAAGIAS"
     gene            4430..>5102
                     /locus_tag="VF276_03675"
     CDS             4430..>5102
                     /locus_tag="VF276_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019503017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX6605993.1"
                     /translation="MKAAVVEQPGGPEVLQIKELPVPGPRPGWVLIKVKAFGLNRSEM
                     FTRQGHSPSVRFPRVLGIECVGVVADGGGTGLAPGQKVAAVMGEMGRAYDGGYAEYTL
                     VPASQVMPLTTDLPWTTLAAIPETYLTAWGSLVEALDVQAGQQLLIRGGTSALGMAAL
                     SLAKDMGLYVAATTRTGAKVAALVANGADDVIIDTGQLAQAAHERIPAGFDCTLDLIG
                     TVTLLD"
BASE COUNT          746 a         1868 c         1738 g          750 t
ORIGIN      
        1 cagttgctgc gccgccagcg cgtcctgctg gtggtcgata acttcgagac cgtgaccgac
       61 cgcagcctgc tgccctggct gctgcgcctg cccgagccga gcaaggcgct ggtcaccagc
      121 cgcgaatact cgcgcgcctt ccgcaacaac accttcgtgc tcgacctgcg cggcatgagc
      181 gagaccgaag cccaggaact gatcgcccag cgactgcgga tgctgcgcat ggacgcgctg
      241 gtgcgcgacc ccgccgaact ggccccgctg gtggcggcca ccggcggcaa ccccaaggcc
      301 atcgagatca gcctgggctg cctcaagtac gagcaccgcc ccttccggca ggtgatcgac
      361 gacctctacg cggcgcgggg cgaactgttc gaggatctgt tctcgcgctg ctgggcgctg
      421 ctcgacgagg ccgcccggcg cgtcctgctc gccatgccgc tctttctcga cagcgccggg
      481 cgccccgccc tggccgccac cgccgacgtg cagggcttcg ccttcgaccg cgccgtggag
      541 cgcctgaccg acctggccct gctcgacgcc caccagcccg acctgcacag cgcgccccgc
      601 tacacgctgc acccgctggt gcgcgccttc gccgcgtccc ggctgcgcga gcaacccgac
      661 tgcgaggccg ccgcacgcga gcgctggatg gactggtacc gatgcctggt ggcccaggtc
      721 ggctactgct gggacgacct gacccggctg gaactgctcg acgcggagca ggacaccgtc
      781 cacgccgtgg cgcagtggac cttcgagcac caccgctacg ccgatacgct ggcgatggcc
      841 cgcggcgcgg ggtactacta ctacgtgcgc gcgctgtggg acaagcagcc gcacatcaac
      901 ctgctgcgcg ccgaagccgc ccgccacctg ggcgacctga ccggcgagat cgaggctctc
      961 gcctaccatg tgcacatgct cagccgccag ggtaatgtcg ccgaggccga gacgtacctg
     1021 ggccgcctgg acggcatggc agcgggcgtg ccgttgccgc cggaggtact ttacgaatac
     1081 cagtacgcca ccgcgctcta ctggatggcg cgcggccacc tcgacaccgc cgaagcggcg
     1141 tgccggcgca gcctgggcct ggccgagcac ctgacgccca accagtacgt catcgcccgg
     1201 cagtggctgg cgacctgcct ctaccagcag ggcaagctgc ccgagggcgc cgcgctgttc
     1261 gccgaatcgc tgctggacgc gacgcggctg gggctgcaac gctgcatcat tttcggcatg
     1321 gtgcgcctgg ccgccatcga cctggaccag ggccggatcg acgccgcgct ggcggcattg
     1381 ggcgacagcc aggcccgcgc ccagcagtac caggatcgcg acaacctggc ccagatcgcc
     1441 cggctctacg cccgcgccca tgccctgcgc ggcgacctgg cggcggcgcg cggcgcgctg
     1501 accacggcgc tggacctctt cgagcgcctg ggcatgcgcc gcgaactggc cgaggcccgc
     1561 gccgaactgg cccgcctggc cgccccggac gccgccgcgc ccaacgccgc gcccggtctg
     1621 ccccagccgc ccgccgccgc gcgccgctga ggcctgcggc gctgctctgg ttcttgtcac
     1681 tctgagcgca gcgaagagtc tcgtgaggca ggagacgaga tgcttcactg cgttcagcat
     1741 gacaacgggg agtgggccaa cgatactgca tagcaggagg aacccgtttg tgaccacact
     1801 ggacccggat cttgatgcgc tgattcgcgc catgcccaag gccgaactcc atgtccacct
     1861 ggaaggatcg gtgcggcccg ccaccctgct gaccctggcc cggcggcacg gcgtcgatct
     1921 gggctgccag gacgaggcgg ggctgcgcga gttgtaccgc ttccgcgatt tccgccattt
     1981 catcgacctc tacaaggtga tcagcgaggc catgcgcgcg ccggaagact ttgtgctgat
     2041 catcagcgaa ctcgggcgcg acgcggcggc ccagggtacc cgctatgtgg aaacgacctt
     2101 cagcatcgcg cctcacgtgc gtaacaaggg cctgcccgcg gacgagattt tggacgccat
     2161 cggcgcggcg gcggacgcca cgcggcggga gacgggcgtg gagatccgct tcatcctgga
     2221 ccacgtgcgc atggccccac ccgacgagtg ccggcaggtg gccgagtggt gcgtggctgg
     2281 gcgcgaccgg ggcgtggtgg cgctgggact gggcggctac gagccggaac gcccggcgtc
     2341 gctgttcgcc gagtcgatcc gttgggcgca ggcgcagggg gtgccgttcg tgccccacgc
     2401 gggcgaggcg gtggggccgg agggcgtgtg ggatgcgctc cagttcgacc cgccgcgcat
     2461 cggccacggc ttccgcgccg ccgaggaccc ggccctggtg gccgtcttgc gcgaccgcag
     2521 cgtggtgctc gaaatctgcc cgaccagcaa cgtctgcaca gccagcgtac cgagcctggc
     2581 cgcccacccg ctgcgcaccc tgtgggacgc gggcgtgccg atctcgctca attcggacga
     2641 cccgccgatg ttcaacacca cgctggtcga cgagtaccgc gtggccgcca cgcacttcgg
     2701 cttcacgccc gccgacctgg cccaggtgag ccgcacggcc atcggcgctg cgctgctgcc
     2761 ccctgccgag cgcgcccggc tggtggcgga gttcgacgca gagttgcggc ggctggggct
     2821 gtaggcgtat actggcgcca tgcatctaca cctgttcagc gggggcggcc tcgccatgac
     2881 tgcgacggtg gtggcgcgcg ccgccgagcg cctggcaggc gtgcctcacc cggtcatcgg
     2941 ctggattccc gccgccagcg tggacccgcc gggcgcctgg atcgactaca tgacccaggc
     3001 tttcgccccc gtgggcacca tccggctcat cgacctcgat ctggccgatg cggcgagttt
     3061 cgccgcggac atcgccgcct gcgccgccgt cttcgtgccc ggcggcaata cttacctgct
     3121 cagcaagcgc atgcaccgcc accgggccat gggcacgctg agcgacgcgg tgcgcggcgg
     3181 cgtgccgttg ctcacgcgca gcgcgggcac ggtcttctgc ggccccaaca tgctcaccac
     3241 caacgactgg aacgtcttcg gcaccaccga gttcgacgcc ctgggcctca gcccctacaa
     3301 cttcaacgtc cacttcactc tgccgcccga agacgaaccc gagtcgcgcg atttccgtat
     3361 ccgccagtac atcgccgtga accggcgccc ggtgttggcc ctcgaagaga cctgcaacct
     3421 ggagtggacc gacagcgcag tccgggtcca cggcgccacc tgctggctct acacgacgag
     3481 cggcgagcgc cgccccctgc cgccgggcag caccttctcg ccctccctgg gaggctagga
     3541 tgcaagccct cagcatgtcg atcctcatcc gccccgcgcc caaacccgac cggcacatcc
     3601 cttatggccc cgacccgcag cagttcgggc acctgcgctt gcccgcggga cccggcccgc
     3661 acccgctggc cgtcgtgatc cacggcgggt tctggcgcaa cgcctatgac ctgaaacata
     3721 tcggccacct ctgcgcggcg ctggggcgcg ccgggctggc gacctggagc ctcgaatacc
     3781 gccgcctggg cgatgcgggc ggcggctggc ccggcacgtt ccaggacgtg gcgcggggca
     3841 cggatcatgt gcgcgccctg gccgcggagt atccgctcga cccggcccgc gtgatcacgg
     3901 tggggcactc ggcgggcggc cacctggcct gctggctggc cgggcggcac cgcatcccgc
     3961 cccgcgacat cctgcatagc cccgacccgc tgcccctcgc cggcgctgtc gcgctggcgg
     4021 gggtgctcga cctggcgcgg gcctggaatc tgcgcctcag caaaggcgtc gtctgcgagt
     4081 tcatgggcgg cgccgccgag gagatgccgg aacgctacgc cacggcctcg cccatcgaac
     4141 tgctgcccct cggcgtgccg cagtggctgg tccacggcac cgaggacggc aatgtgccct
     4201 acgagatcag cgagacctac cacgccgccg cgctggccgc cggggatgac tcgcatctca
     4261 tcctgctgcc cggcgccggg cattttgagg tgatcgatcc acgttcgcgc gaatggccgc
     4321 aggtccagga agcgatcctg tccgccgcgg gtatcgcttc ctgattcgga ttcgcaaagg
     4381 cgccgcggag gctgggattt taccgattgg cacggagaga ttcacgctaa tgaaagcagc
     4441 ggtagtagag caaccgggcg gtcccgaggt cttgcagatc aaggagctac cggtgcccgg
     4501 cccacggccc ggctgggtgc tgatcaaagt caaggcgttc ggcctgaacc gctcggagat
     4561 gttcacccgc cagggccatt cgcccagcgt gcgctttccg cgcgtgctgg ggatcgaatg
     4621 cgttggcgtg gtcgcggatg gcggcgggac gggcctggcg ccggggcaga aggtcgcggc
     4681 cgtgatgggc gagatgggcc gcgcctatga cggcggctac gccgagtaca ccctggtgcc
     4741 ggcaagccag gtgatgccgc tgacgaccga tctaccctgg acgacgctgg cggcgatccc
     4801 cgaaacctac ctgaccgcct gggggtcgct ggtcgaggcg ctcgatgtgc aggccgggca
     4861 gcagttgctg atccgcggcg gcacctcggc cctcggcatg gcggcgttga gcctcgccaa
     4921 agatatggga ctgtacgtgg cggcgacgac ccgcaccggc gccaaggtcg ctgcgctggt
     4981 cgccaacggc gcggacgacg tcatcatcga cacggggcag cttgcccagg cggctcacga
     5041 gcgaataccc gcgggcttcg actgcacgct ggacctgatc ggcacggtga ctctgctgga
     5101 ct
//
LOCUS       DASUYC010000225         4903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1899159, whole genome shotgun sequence.
ACCESSION   DASUYC010000225 DASUYC010000000
VERSION     DASUYC010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4903
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1899159"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VF276_03680"
     CDS             <1..545
                     /locus_tag="VF276_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020640346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX6605994.1"
                     /translation="ESNLLGLALAAAVGIFLLLQLSFGSWRLATLSFLTLPSALVGGL
                     LAAFAAGGIISLGSLVGFFTVFGIAARNGILMINHYQHLERYEGETFGMGLVLRGARE
                     RLAPILMTALATGLALVPLVIAGEIPGHEIEYPMAVVILGGLVTSTVLNLFIIPAIYL
                     LFGRDKNPSAPVSAIPASGE"
     gene            746..1795
                     /locus_tag="VF276_03685"
     CDS             746..1795
                     /locus_tag="VF276_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605995.1"
                     /translation="MRGLHIVALAGALFLLISTGAYAAGPGPAAPLISDPPVKDYEDF
                     NPNNFDHPTQINNPWLPFTPGRQLTYDGFTVEDDGSLLPHRVVRSATDMTKVIDGVRT
                     VVGWDADYSDGVLKESELVFRAQDNDGNVWHFGEYPEVYEEGKITPNQVWLHGFEEAH
                     AGIAMWGNPQVGTPSYSEGWAPVVDWTDRGKVDQVGQKTCVPAGCYDDVLVIAESSRT
                     EPNAEQIKYYARGVGYVRIGWRGTGPQSKETLELTSVKMLSADELAQVRATALALEQR
                     SYKNSPTVFALTTPIELPPGVSRPPIPSDGGAPGMPRTGRAANPVDLWVIIGFMIGLL
                     GAAGGLVLRRRAGLR"
     gene            1980..2948
                     /locus_tag="VF276_03690"
     CDS             1980..2948
                     /locus_tag="VF276_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018773123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605996.1"
                     /translation="MSRGQRGWSVGLSAVFVFLMLLTSTGVALAGPIDDSSDPSESTI
                     DNRSIPCSQPVEFNRHDFPNRPVIDNQWTPFVPGTQFILEGRANRGGGPLPHRVVLTV
                     TDLTKVINGVRTIVSWDRDFGEATLAPNIPDVEDPETLLSSLQIQESELAFNAQDEDG
                     NVWNMGEYPEEYENGHLTGAPNTWIPGVQGAKAGLLMRAHPRLKTSRYLQGYSPRIDF
                     LDCAKVFQTHQRVCVPANCYPDVLVTDENSPLAPEDGHQRKFYAAGVGNVQITAVDDP
                     EGETLVLVKINHLSARALATIHRKALELDTRGYQISEVYRHTEHAR"
     gene            3029..3889
                     /locus_tag="VF276_03695"
     CDS             3029..3889
                     /locus_tag="VF276_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605997.1"
                     /translation="MLNPFHRRCGRGILSLLGLLSGLLPGVAAAAPVHQGAVDPANFV
                     AQVDNPYFPLTPGRTWVYEGARDGHAQRAVVTVTGATKTILGVQCLVIQDDVTEEGQI
                     VEQTEDWYAQDKAGNVWYFGESFKERLDDGSFSTQGSWLAGVDGVQPGIIMEGQSHLG
                     DNYNQEQASGVAEDKARVIGLNQSVTVRYGAFRDVLVTEEYTPLEPGFVEHKYYAPGI
                     GNVLTVATQGGQERMELVSVTTTAPGMPRTGGAGRPGSPWVLLAALLGLLSVAGGLAF
                     RRRRAAAGPI"
     gene            complement(4064..4606)
                     /locus_tag="VF276_03700"
     CDS             complement(4064..4606)
                     /locus_tag="VF276_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605998.1"
                     /translation="MLTPENAAAATQAVQQYHELQTRTALTPTEPPPPTIQEPPTPTL
                     GMGQITDSDCMERVYSSGPHFPSCWETQINGQWWLVGGGQVGGNTETPLQSIIIVCPE
                     RCYDPTTGTIYETPSNVLEVRIATISGPLVTVVPRDPARPERFFFDLLTRQWVSGPDG
                     APPPAASPSLAPLPTASVQA"
BASE COUNT          861 a         1546 c         1656 g          840 t
ORIGIN      
        1 cggagagcaa tctccttggc ctggccctgg ccgcggctgt cgggatcttc ctgctgctgc
       61 aactgtcgtt cggaagctgg cggctggcga ccctctcctt cctgacgttg ccctcggccc
      121 tcgtgggcgg gctgctggcc gcgttcgccg cgggcggcat catctcgctc ggctcactgg
      181 tcggcttctt caccgtcttc ggcatcgccg cccgcaacgg catcttgatg atcaaccact
      241 accagcacct ggagcgctac gaaggcgaaa ccttcggcat gggcctggtg ctgcgcggcg
      301 cacgcgagcg gctggcgccg atcctgatga ccgcgctggc gaccgggctg gccctggtgc
      361 cgctggtgat cgccggcgag atccccggcc acgagatcga ataccccatg gccgtcgtca
      421 ttctgggcgg gctggtcacg tccaccgtgc tcaacctgtt cattatcccg gccatctacc
      481 tgctgttcgg gcgggacaag aacccgtccg cccctgtatc ggccatcccg gcgagcgggg
      541 agtgacgccg caggcaagac ctgccgggct aaaggaccgt tgaacggtgc ttgcctatct
      601 gcctcctgtt gggcgtagat aggcaagcgc accggcaaca acagaaaagg tctcttgccg
      661 gcgggtgccg gcttctaccc gcaccgcaga tcgtacactc tagccgccgg taaccggcaa
      721 tggatcatat tcagaggaga taaagatgcg cggccttcac atcgttgctc tcgcgggtgc
      781 gttgtttctg ctgatctcga cgggggctta cgcggcggga cccggccccg cggcgccgct
      841 tatcagcgac cctccggtga aggattacga ggacttcaac cccaacaatt tcgaccaccc
      901 gacgcaaatc aacaacccgt ggctgccgtt cacgccgggg cggcagttga cgtacgacgg
      961 cttcacggtg gaggacgacg gcagcctcct gccgcatcgc gtagtgcgca gcgccaccga
     1021 catgaccaag gtgatcgacg gcgtccggac ggtcgtcggc tgggacgcgg actacagcga
     1081 cggcgtactg aaggagtccg aactcgtctt ccgggcgcag gacaacgacg gcaacgtgtg
     1141 gcactttgga gagtaccccg aagtatacga ggaaggcaag atcaccccca accaggtctg
     1201 gctgcacggc tttgaagagg cccacgccgg gatcgcgatg tggggcaacc cgcaggttgg
     1261 tacgcccagc tattccgaag gatgggcccc cgtggtggac tggacggatc gcgggaaggt
     1321 cgaccaggtg ggccagaaga cctgtgtgcc cgcgggttgc tatgacgacg tgctggtgat
     1381 cgccgagagc agccggaccg agccgaacgc cgaacagatc aagtactatg cccgcggcgt
     1441 gggctatgtg cggatcggct ggcgcgggac cggcccgcaa agcaaggaga cgctggaact
     1501 gacctcggtc aagatgctca gcgccgatga actggcgcag gtgcgcgcga cggccctggc
     1561 cctggagcag cgctcctaca agaacagccc aaccgtgttc gcgcttacca cgcccatcga
     1621 gttgccgcca ggcgtgagcc gtccgcccat tccaagcgac ggcggcgcgc ccggcatgcc
     1681 gcggacaggg cgggcggcaa acccggttga tctctgggtg atcatcgggt ttatgatcgg
     1741 gttgctgggg gccgcgggcg gcctggtttt gcggcgccgc gcggggctac gctagccggc
     1801 actgcggtac gccggccaac ccggatagcg gacgcggccc gcgttggaaa tgcggtgggg
     1861 caggcaaatc gcctgctcca ccgcacaggt aatcaggaca atctgcaaga aggagtgttg
     1921 cacgcacccg ttgttctcgc ggccaccagc acgcctatcc ggcgagaaag gatgcttcga
     1981 tgtctagagg acagcgcgga tggtcggtcg gcctgagcgc ggtttttgtg ttcctgatgc
     2041 tcttgacgag caccggcgtt gccctggcag gccctattga cgatagcagc gatccttctg
     2101 agtctaccat cgacaatcgc tccataccct gctcgcagcc ggtggaattt aaccgccacg
     2161 atttcccgaa ccggccggtg atcgacaacc agtggacgcc cttcgtaccg ggcacgcagt
     2221 tcatcctgga ggggcgcgcc aaccggggcg gcgggccgct gccccatcgc gtggtgctca
     2281 ccgtcaccga cctgaccaag gtgatcaacg gcgtgcgcac gattgtctcg tgggatcgcg
     2341 actttggcga ggccacgctg gcgccgaaca tccccgatgt tgaggatccg gaaacgctcc
     2401 tcagttcgct ccagatccag gagtcggaac tcgcctttaa cgcgcaggac gaggacggca
     2461 acgtctggaa catgggcgag tatccggaag agtacgagaa cggccacctg accggcgccc
     2521 ccaatacctg gatccccggt gtgcagggcg cgaaagccgg cctgctgatg cgcgcccatc
     2581 cgcggctgaa gacatcccgc tacctgcagg gctactcgcc gcgcatcgac ttcctggatt
     2641 gcgccaaggt gttccagaca caccagcggg tctgtgtgcc ggccaactgc tacccggatg
     2701 tgctcgtgac cgacgagaac agcccgctcg cgccggagga cggccaccag cgcaagttct
     2761 acgcggcggg ggtcggcaat gtgcagatta ccgccgtgga tgatccggag ggcgagacgc
     2821 tggtgctggt caagatcaac cacctcagcg cccgcgccct ggcgaccatc caccgcaagg
     2881 ccctggagct ggatacgcgc ggctaccaga tcagcgaggt gtatcgccat accgagcatg
     2941 cccggtagtc gccatcggcg gttgaacaaa ccggcctgtg cgaggagcgg ttgcggcctt
     3001 ccgctccgct atcggaaagg agacgcctgt gttgaatcca ttccatcgcc ggtgcggtag
     3061 ggggatctta tcgctgctcg gtctgctctc cggcttgctg cctggcgtgg ccgcggcggc
     3121 accagtccac cagggggcgg tcgacccggc gaatttcgtg gcgcaggtcg ataacccgta
     3181 tttcccgctg acgccgggcc gaacctgggt ctacgaaggc gcccgcgacg gccacgcgca
     3241 gcgggcggtg gtgaccgtga ccggcgccac caaaacgatc cttggcgtcc aatgcctggt
     3301 catccaggac gacgtgacgg aggagggcca gatcgtcgag cagacggagg actggtatgc
     3361 ccaggataag gcgggcaatg tatggtactt cggcgaaagc ttcaaggagc ggctcgacga
     3421 cggcagcttc agcacgcagg gttcctggct ggcgggtgtc gacggcgtgc agccggggat
     3481 tatcatggaa gggcagtcac atctcggcga taactataat caagagcagg caagcggggt
     3541 tgccgaggac aaggcgcggg taatcggcct gaatcaatcg gtgacggtgc gctatggtgc
     3601 gttccgcgat gtgctggtga cggaagagta cacgccgctg gagccgggct tcgtggagca
     3661 taagtactac gcgcccggca tcggcaatgt gctgacggtg gcgacccagg gcgggcaaga
     3721 gcgcatggaa ctcgtgagcg tgaccaccac cgcgcccggt atgccgcgga ccggtggggc
     3781 cgggaggccg ggttccccct gggtattact ggcggcgctg ctcggcctgc tcagtgtagc
     3841 ggggggcctg gcttttcggc gcaggcgggc agcggccgga cccatctgag ccgcagggtc
     3901 gctcgccgtg cccatgcgcg gcacccggca tccgggcgcc acgcgccggc atggggccac
     3961 gccgtaacac gcggcgtgcc gcgagcaccc gctcgcgccg ccgaattccg atacggcgtt
     4021 ggccgcgccg tcccactgta ggtttggatt gcatcacagg cctctacgcc tgcaccgatg
     4081 cggtcggcag cggcgcgagc gacggcgacg cggcgggggg cggggcgcca tccggcccgc
     4141 tcacccactg gcgcgttagc agatcgaaaa agaagcgctc cgggcgggcc ggatcgcgcg
     4201 ggacgaccgt gaccaggggg ccgctgatag tcgcgatacg gacttccagg acattgctcg
     4261 gcgtctcata gatggtaccg gtggtgggat catagcagcg ctctgggcag acgatgatga
     4321 tgctctgtaa gggcgtttcg gtgttgccgc cgacctggcc gccgcccacc aaccaccact
     4381 gcccgttgat ctgagtttcc cagcaactgg gaaagtgggg gccactggag tagacccgtt
     4441 ccatacaatc gctgtcggtg atctgcccca tccccagtgt cggggtcggc ggctcttgga
     4501 tggtcggcgg cggcggttcg gtcggagtga gcgccgtgcg cgtctgtagt tcgtgatact
     4561 gctgcacagc ctgggtagcg gcggcggcgt tctcgggagt cagcaatgcc cccgaaggat
     4621 agcccccttt gagttcctca aatacggacg taggatcacg cgtaggattg ttatccggca
     4681 tccgactcgc ggttattgcg atcgaagtag gactcggccc cggtgcatta gtgtaagtcg
     4741 gcacagcggt actgacagca gtagccgcag gcaaggtggt tgtgccacag gcgctaacat
     4801 atagcgcgat gcaccccgtt agggccaaca cctggatttt catcgtactt cgcattggcg
     4861 tgcgtcgatc tccatgcttc actatcaggg cgccgggacg ggg
//
LOCUS       DASUYC010000226         3507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_48582,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000226 DASUYC010000000
VERSION     DASUYC010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48582"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VF276_03705"
     CDS             <1..294
                     /locus_tag="VF276_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6605999.1"
                     /translation="DIQAAHRDRARGEYGAEEEPLYGLEALPGGHGEAPIEESVAEEA
                     ASEAGRPGEQPGLNLTWGIILGGVLLLVLVTAVLFIGVGQTYQADIVTHYGVK"
     gene            308..622
                     /locus_tag="VF276_03710"
     CDS             308..622
                     /locus_tag="VF276_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606000.1"
                     /translation="MAYVRTMTFVVPPERTHELQPGHNLFMAAVNGTQIVAQNTPGFH
                     RGGVWMQNLPQGSIKVVCYTQWYGVPDIDRYASTPMIRDFEADVARYHSTPVIEVYET
                     LS"
     gene            632..892
                     /locus_tag="VF276_03715"
     CDS             632..892
                     /locus_tag="VF276_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606001.1"
                     /translation="MWSFISWTNLEDLLLDMGAVLVVTVLVTIWAVANNRRAERMHHI
                     PQPEPGPLPPLPVPQPEPPSPVPGPPHPQPPPPEPPLPGSPT"
     gene            908..1444
                     /locus_tag="VF276_03720"
     CDS             908..1444
                     /locus_tag="VF276_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606002.1"
                     /translation="MPTSLHTWRKIMYPLIGILILIVFGLSLYETYVTNFGPDPSSAI
                     GVPAVGGVLPRDDQRGAYVQLKPGADPRFPSSLVQLFDSDTPGLFTADEAVQLDGHAI
                     DAVIVSQSTIDQDAAQYRVYAVGQDTQETTAQRLNSGRMIRIQPKSGLWAPGSYVVDV
                     PSGGMFDTSRTYYTFTIK"
     gene            1500..>3507
                     /locus_tag="VF276_03725"
     CDS             1500..>3507
                     /locus_tag="VF276_03725"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX6606003.1"
                     /translation="MSAIATPKNGSFAPSGYADDRAEVAVDTVNADHGLPRFFFAAGV
                     ALVISAIHGILQRLPIFAEWIRTADYGGHMITNLAHTHITIVGAGTISLTALLYYIMP
                     RLGRRPLYSGTLTSVSFWFTVFGVFGFYLSVLAVGAYEGMMVHAGWQYEAARDYLGAW
                     HKAPIAITAAIMGIGYWTFTANVWITARNIGKFRRENPKEGATPIEWLLVQFCVVCAT
                     ALFIGTVQGVYQVMPWSLDWLRAAGHAGELIDPMAHAHMNLVGGVSVGMMALLYFFLP
                     RITGRPIYSYNLARFSYYSVVIGVFGFWLVAITLGFVEGTPVVEGRMTYDQIRAQVGL
                     WHTIPLVTVATIMGIGFWAFIANIFLTLRQGPAPDAPSDRWLARFVGLSVFGLLLGTI
                     QGVLQTIPVIHDWLEEAEGTGWMVMPFAHAQLNMVAFAILGLVTLSTFALPRVTGRPL
                     FSVPFARRTLAVIASGIFATYVVFITLGLLETRAFYQMFPQGEHGASGAMDDFLPPNT
                     AVIVRSAVGGDFVHTALVLLVACWVGLGYFMLMRHIAGSIGREAIQAYWDAFRGRTAA
                     AAHQHSMPHPSAVPQDVRQVRRRGLQLAFYEVAAGWAGFLGMGWLLSGRGFIGVMVLS
                     VWAGIYWSTLFILLAVTAVGPVGIAITAILWIGLPLLSAVAVYRT"
BASE COUNT          535 a         1212 c         1121 g          639 t
ORIGIN      
        1 gatatccagg cggcccaccg cgaccgggcg cggggcgaat atggcgccga ggaagagccg
       61 ctgtatggcc tggaagcgct gcccggcggg cacggcgagg cgcccatcga ggagagcgtg
      121 gccgaagagg ccgcgagcga agcgggccgc cccggcgaac agcccggcct gaacctcacc
      181 tggggcatca tcctcggcgg ggtgctgctg ctggtgctcg tcaccgccgt gctgttcatt
      241 ggcgtcggtc agacgtatca ggccgacatc gtgacccatt acggggttaa gtagaggtta
      301 agaccgcatg gcctatgtac gtaccatgac ctttgtcgtg ccgcccgaac ggacccacga
      361 gttgcagccg ggccataacc tgttcatggc ggccgtgaac gggacccaga ttgtggctca
      421 gaataccccc ggctttcatc gcgggggggt ctggatgcaa aatctgccgc agggcagcat
      481 caaagtcgtg tgctacacac agtggtacgg cgtgcccgat atcgaccgct atgccagcac
      541 accgatgatc cgcgatttcg aggccgatgt ggcgcgctac catagcacgc cggtcatcga
      601 agtctatgaa acgctgagct aaggccctgg gatgtggagc ttcattagct ggaccaacct
      661 ggaagacttg ctgctggaca tgggcgccgt cctggtcgtc accgtgctgg tgacgatctg
      721 ggccgtggcg aacaaccggc gggcggagcg gatgcaccat attccgcaac ccgagcccgg
      781 cccgctgccg ccgctgcccg tgccccagcc cgagccgccc tcgccggtcc ccggcccgcc
      841 gcatccccaa cccccccccc cggagcctcc gcttccaggg agccccacct agaaacgagc
      901 cgcacgcttg ccgacttccc tgcacacctg gcgcaagatc atgtatccgc tgatcggcat
      961 actgatcctc atcgtctttg ggctgtcgct ctacgagacc tatgtcacca actttggccc
     1021 cgaccccagc tccgccatcg gcgtgcccgc cgtgggcggc gtgctgccgc gggacgatca
     1081 gcgcggcgcc tatgtgcaat tgaagcccgg cgccgatccc cgcttcccct ccagcctggt
     1141 gcaattgttc gactccgata cccccggcct cttcaccgcg gacgaagcgg tgcagctcga
     1201 cggccacgcc atcgacgcgg tgatcgtcag ccagtcgacg attgaccagg atgccgcgca
     1261 gtaccgggtg tatgccgtgg gccaggacac ccaggagacg acggcccagc ggctcaacag
     1321 cggccgcatg atccgtatcc agcccaaatc cggcctgtgg gcaccgggat cctatgtggt
     1381 cgatgtgccg tccggcggca tgttcgacac cagccggact tattacacct tcaccatcaa
     1441 atagggacgc caggggccgt tgcggtagag tcgcgcccct tatcacagga gcctgcatcg
     1501 tgagtgctat tgctacgccc aaaaacggca gctttgcccc ctccggttac gccgacgacc
     1561 gcgccgaggt cgccgtcgat accgtcaacg ccgatcacgg cctgccgcgc tttttctttg
     1621 cggcgggtgt cgcgctggtg atcagcgcga tccacggcat tttgcaacgc ctgccgattt
     1681 ttgccgagtg gatccgcacc gccgactacg gcggccacat gatcactaac ctggcccata
     1741 cccatatcac catcgtgggc gccgggacga tcagcctgac ggccctgctc tattacatca
     1801 tgccccggct ggggcggcgg cccctctata gcggcacgct gaccagcgtg tcgttctggt
     1861 tcaccgtctt cggcgtgttc ggcttctatc tctcggtgct ggccgtcggc gcctacgagg
     1921 gcatgatggt ccacgccgga tggcagtatg aagcggcgcg cgactatctc ggcgcctggc
     1981 acaaggcgcc catcgccatc acggcggcga tcatgggcat cggctactgg accttcacgg
     2041 ccaatgtctg gatcaccgcc cgcaacatcg gcaagttccg ccgcgagaac cccaaggagg
     2101 gcgccacgcc tatcgagtgg ctgctggtgc agttctgcgt ggtctgcgcc acggcgctgt
     2161 tcatcggcac ggtgcagggc gtgtaccagg tgatgccctg gtcgctcgac tggctgcgcg
     2221 cggcagggca cgcgggcgaa ctgatcgacc cgatggccca cgcgcacatg aacctcgtcg
     2281 gcggcgtgtc ggtcggcatg atggcgctgc tctacttctt cctgccgcgc atcaccgggc
     2341 ggcccatcta cagctacaac ctggcccgct tcagctacta cagcgtcgtc atcggcgtgt
     2401 tcggcttctg gctggtggcg atcaccctcg gcttcgtcga gggcacgccg gtcgtcgaag
     2461 ggcgcatgac ctatgaccag atccgcgcgc aggtggggct gtggcacacc attcccctgg
     2521 tgacggtggc gacgatcatg ggcatcgggt tctgggcctt catcgccaat atcttcctca
     2581 cgctgcgcca ggggccggcg cccgacgcgc ccagcgaccg ctggctggcc cggtttgtcg
     2641 gcctcagcgt cttcggcctg ctgctcggca cgatccaggg cgtgctccag acgatcccgg
     2701 tgatccacga ctggctggaa gaggccgagg gcaccggttg gatggtcatg ccgttcgccc
     2761 acgcccaact gaacatggtg gccttcgcta tcctgggcct ggtcacgctc tcgaccttcg
     2821 ccctgccccg cgtgaccggg cggcccctgt tcagcgtgcc gttcgccagg cgcacgctgg
     2881 ccgtgatcgc cagcggcatc ttcgccacct acgtggtctt catcacgctg ggcctgctgg
     2941 aaacgcgggc tttctaccag atgttcccgc agggcgagca cggcgcctcc ggcgcgatgg
     3001 acgacttcct gccgcccaac acggcggtca tcgtgcgctc tgccgtcggc ggcgacttcg
     3061 tgcatacagc gctggtgctg ctggtggcct gctgggtggg cctgggctac ttcatgctga
     3121 tgcgccacat cgccgggtcc atcgggcggg aagcgatcca ggcctactgg gatgctttcc
     3181 gcgggcgcac ggccgccgcc gcgcaccagc actcgatgcc gcaccccagc gccgtgccgc
     3241 aggatgtgcg gcaggtgcgg cgccgcggcc tgcaactggc cttctatgaa gtcgccgcgg
     3301 gctgggccgg gttcctgggc atgggctggc tcttgagcgg gcgcggcttc atcggcgtca
     3361 tggtgctctc ggtctgggcc gggatctact ggtccaccct gttcatcctg ctggctgtga
     3421 cggcggtggg gccggtgggc atcgccatca ccgccattct ctggatcggc ctgccgctgc
     3481 tcagcgcggt ggccgtctac cgcacgt
//
LOCUS       DASUYC010000227         3277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_279987,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000227 DASUYC010000000
VERSION     DASUYC010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279987"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            853..1611
                     /locus_tag="VF276_03730"
     CDS             853..1611
                     /locus_tag="VF276_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX6606004.1"
                     /translation="MSGHSKWATIKHKKEMTDNKRGQLFTKLTREITVAAREGGGDPD
                     TNFRLRLAVQRAKAESKPADNIKRAIERGAGGAAGGANYEEVSYEGYGPSGTALIVSA
                     LTDNRNRTVAEVRAAFTRGGGTLGEAGSVGWMFDTMGLITVEVDPKQDMDEAELAAID
                     AGAQDVKRDDDERTLEVYTEFTDLKAVTDKLTAAGLNVTSSEKAMVPKTTVQLDEDKA
                     TQNLKLIDRLEDLDDVQQVYSNLELSDAQVAALG"
     gene            1738..2325
                     /gene="ruvC"
                     /locus_tag="VF276_03735"
     CDS             1738..2325
                     /gene="ruvC"
                     /locus_tag="VF276_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007133160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX6606005.1"
                     /translation="MSTVLYPLDSVNPDLVLGIDPGTAIMGYGLVSHAVAQQQEAPGG
                     AAWLGMSGLQGLEYGALRTPAHMPLVERLPRLYGGLIELIREYQPSAVAIEALFFGRN
                     VTTAISVGQARGVAVLAAAHCGVPIAEYTPMQVKQAVVGYGKATKDQVQQMVRMLLNL
                     ESIPQPDDAADALAIAICHLHSRSLDTLLAQQSQR"
     gene            2346..3164
                     /locus_tag="VF276_03740"
     CDS             2346..3164
                     /locus_tag="VF276_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEX6606006.1"
                     /translation="MAKQKQRLDLLLVERGLAETRERARALIMAGAVTANGRRADKPG
                     MAFPVESSLEVKEALPYVSRGGFKLAGALAAFGLGPRVAGAVALDAGASTGGFTDVLL
                     QHGAARVYAADVGSGQLAWKLQTDPRVVVMDDVNVRHLDALPEPVGVVTADLSFISLS
                     LVMPALIRVSAPDAWMVLLVKPQFEAGREQVGKGGVVRDRRVHRAVLERVTAEWQALG
                     LTVAGLARSPVLGPAGNVEFLAYLEKQAPAEAPLPVAAAIDHALAAPAESESPA"
BASE COUNT          586 a         1012 c         1112 g          567 t
ORIGIN      
        1 gtacatgctt ttgtctaaat cgtaccaaga taaggctgaa tgcctcttga catcaatcta
       61 acgataaggt atagtaaggt tgcctcgatg gtccataagg tgatgtaccc cgctcacgcg
      121 catagctatg gtagtggtga ccgctttgca gccacgaact ggctgtgctc ttggggatag
      181 gcaatgtcgc ttagaaagga ggtacagtat ggcaggaaag ctctcccatc tttccactcg
      241 ccaaaaggtc ggcttagcta tagctgtagt gtttctgctg gttagcgtgg ttgcatccgg
      301 ttccgccctt gctcagaact catcaactgg gcggccagat cccaacggct tcgttaagaa
      361 tgtgttccac gctgtggtca cgcccaaccc tactgaggta gcccgtagct accaggagtg
      421 gcaagagcta ctcaagcacc aggatcctgc tgcttcgcca accccctcga atccctaatc
      481 ttcccctata acacggccac ctcggtccac aatggtctaa acttgtcctc cgactctcag
      541 tattgttttc aggcgtattt gtcactctga gcggagcgaa gagtctcgtc ttggacctac
      601 acgagacgag attcttcgct gcgctcagaa tgacagggcg cggttggcgg tctactcagg
      661 ggcccacttt cccacttcac cgcggttgca ggaagcgcgg gatgcggtgt ataatatgtg
      721 gcaattcgta ccctggggcc gggccgcctg cgtgcctgct ctcttttccg ccggggccgg
      781 tccctgtttg ccgcagcgga gttcctcttc tacgtccgcg cactattacc gtataaagga
      841 ggatgggcca ggatgtctgg acattctaaa tgggcaacca ttaagcacaa gaaggaaatg
      901 acggacaaca agcgcgggca gcttttcacc aagctgacgc gcgaaatcac ggtggcggcc
      961 cgcgagggtg gcggcgaccc ggataccaac ttccggttgc gcctggccgt gcagcgcgcc
     1021 aaggccgaga gtaagcccgc cgacaacatc aagcgcgcca tcgagcgcgg cgccggcggc
     1081 gcggcgggcg gcgcgaacta cgaggaagtc agctacgagg gctacggccc atcgggcacg
     1141 gcgctgatcg tctcggcgct gaccgataac cgcaaccgca ccgtggccga ggttcgcgcc
     1201 gccttcacgc gcggcggcgg cactctgggc gaggcgggta gcgtcggctg gatgttcgac
     1261 accatgggcc tgatcaccgt cgaagtcgat cccaagcagg atatggacga ggcggaactg
     1321 gcggccatcg acgcgggcgc gcaggacgtg aagcgcgacg acgacgagcg cacgctggag
     1381 gtctacaccg aattcaccga cctcaaagct gtgaccgaca agttgacggc agcgggcctg
     1441 aatgtcacgt cgagcgagaa ggccatggtc cccaagacca ccgtccagct tgacgaagac
     1501 aaggccacgc agaatctgaa gctgatcgac cggttggaag acctggacga tgtgcagcag
     1561 gtctacagca acctggaact cagcgacgca caggtggccg cgctcggcta ggcgccgggg
     1621 ggccggggcc gctcgatagg gggagtgcga cagcggacaa ggggcgcggg cctcttgtcc
     1681 gcgatctatc cgggaatggc cgcccggtcc cggtggtggc cctggaaagt tggcttcatg
     1741 tccacggtgc tatatccgct cgactccgtt aatcccgacc tggtgctggg catcgatccg
     1801 gggacggcga tcatgggcta cggcctggtg tcacacgccg tggcccagca gcaggaggcg
     1861 ccgggcggcg cggcctggct gggcatgagc gggctgcaag ggctcgaata tggtgccttg
     1921 cgcacgcccg cgcatatgcc actggtcgag cgcttgcccc ggctctacgg cgggctgatc
     1981 gaattgatcc gcgaatacca gccgagcgcg gtagccatcg aggcgctgtt ctttggccgc
     2041 aacgtgacga cggccatctc ggtggggcag gcgcgcggcg tggccgtgct ggccgccgcg
     2101 cattgcggcg tgccgatcgc cgagtacacg ccgatgcagg tcaagcaggc ggtggtgggc
     2161 tacggcaagg ccaccaagga ccaggtccag cagatggtac gcatgttgct caacctggag
     2221 tcgattccac agccggacga cgcggcggac gcgctggcga ttgccatctg ccatctgcac
     2281 tcgcgctcgc tcgataccct gctggcgcag caaagccagc gatgaccggc ggggcgggga
     2341 ctcgcatggc gaaacagaag caacgcctgg acctgttgct ggttgagcgc gggctggcgg
     2401 agacccgcga acgcgcgcgg gcgctgatta tggccggggc cgtcacggcc aacggccggc
     2461 gggccgacaa gccgggcatg gccttccccg tcgaaagctc cctggaagtg aaggaagcac
     2521 tgccctacgt cagccggggc ggcttcaagc tggccggggc gctagcggcg tttggcctgg
     2581 ggccgcgggt ggccggggcg gtggccctgg acgcgggcgc ctcaacgggc ggcttcaccg
     2641 atgtgctgct gcaacacggc gcggcgcgcg tctatgccgc cgacgtgggc agcgggcaac
     2701 tggcctggaa gctgcaaacc gacccgcgcg tcgtggtgat ggacgacgtg aacgtgcgcc
     2761 atctcgacgc gctgccggag ccggtgggcg tcgtcaccgc cgacctgtcg ttcatctcgc
     2821 tgagcctggt tatgcccgcg ctgatccgtg tgagcgcgcc cgatgcctgg atggtgctgc
     2881 tggtgaagcc gcagttcgag gccgggcgcg agcaggtggg caagggcggg gtggtgcgcg
     2941 accggcgggt tcaccgggcc gtgctggagc gcgtcaccgc cgagtggcag gcgctggggc
     3001 tgaccgtggc cgggctggcc cgctcgcccg tgctggggcc tgccggcaac gtcgagttcc
     3061 tggcctacct ggagaaacaa gcgcctgctg aggccccatt gcccgtggct gccgcgatcg
     3121 atcacgcgct ggcggccccg gcggagagcg aatcgcctgc atgatcagta aagtcggctt
     3181 tgtcgtcaat cccaccatcc cggcggcgcg gtccgtcgcc gaagaggtca ccaagatcct
     3241 ccacgcggcg cgggtgctga ccgtcgagtg ttccaac
//
LOCUS       DASUYC010000228         4684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1270624, whole genome shotgun sequence.
ACCESSION   DASUYC010000228 DASUYC010000000
VERSION     DASUYC010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4684
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270624"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VF276_03745"
     CDS             complement(<1..237)
                     /locus_tag="VF276_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005439434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEX6606007.1"
                     /translation="MPDSAAFAVFLTAAVILALTPGPGIFYVLARSLQGGRRVGLASA
                     LGTALGGLVHVVAAALGLSAILATSALAFSVVKYA"
     gene            complement(410..1585)
                     /locus_tag="VF276_03750"
     CDS             complement(410..1585)
                     /locus_tag="VF276_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6606008.1"
                     /translation="MYFKRVLAAAVLSAALLLSACGGEAATSTSAPATAPTEAATSTS
                     APMPTAAATATAAAPTTTEAAPATGTNAAAAPTTYTGPVQKVSLALDWTPNTNHTGFY
                     VAQQKGWYKEQGIDLEILPYSDAASPDALVAAGKADFAISFVEQVVIDRVTGLPVKSV
                     AAIVQHNTSELATLKSSGLDRPAKLAGKRYAGFGTPYEEPVIADVIKCDGGPATPIQN
                     ITTNTGGYQALVAKQADFVWIYKGWEALQAKHDKVDLNEFLVRDFCVPDYYSPVIITS
                     EAFLKDKADVGRRFMAATARGFEFAIQQPDQAADLLIAGNPPGSFPDAALVHESAQYL
                     AKEYQAEAPRWGDQTLQVWSGYPKFMLDTGRLEDANHQKVTSLDYAAMFTNELLPAK"
     gene            1872..2462
                     /gene="ruvA"
                     /locus_tag="VF276_03755"
     CDS             1872..2462
                     /gene="ruvA"
                     /locus_tag="VF276_03755"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX6606009.1"
                     /translation="MIAGLRGRLAGKDADSVVIDCGGVLYRVFVPGSALSELSADGAE
                     VLLHTHLAVREDSMTLYGCTTRRQLDLFETLLNVTGVGPKVALAIIGSMPADALEQAI
                     AQGDLVLLTRVPGVGKKLAQRLVLELKGKLDLAALGVGTPGAVRPTGPQAEVAEALGA
                     LGYNASEVQQVLPKLPQDGSVPVEDLVMQALQQMGR"
     gene            2500..3489
                     /locus_tag="VF276_03760"
     CDS             2500..3489
                     /locus_tag="VF276_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX6606010.1"
                     /translation="MEDLELSAVEAAYARLREVVRRTPVLAWGVTGGAGWQPPAGQRL
                     TLKLEHLQLTGSFKVRGAWNLIADLPAEQRARGLVTASGGNHGLAVAWAAARAGVAAT
                     VFLPQMTPAAQEAKIRSFGAAVFRHGLAWDDAWEGAVAYAAERDLPLVHPFDDPRIVA
                     GQGTVGLELLEQAPDLDLLVVAVGGGGLLGGIAAYAGQRRPDLRIVGVEPTGAASMSA
                     ALRAGEPLALPGVQTIAHTLAPRRVSRLTLDLCRRYVESVVLVEDADLVAAMERLWAD
                     ANLLVEPSGAATLAALMAGRIPGAETAHHVGIVVCGANVDAAPAIALATGRHG"
     gene            3649..4347
                     /locus_tag="VF276_03765"
     CDS             3649..4347
                     /locus_tag="VF276_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606011.1"
                     /translation="MVLMPLFAMIVGFALAIVILNHYAANPARRTHEFIWGISFFLFG
                     LGALAEVAADFLGWTDTMARVYYISGAILAVAYLGLGEAVLLWPGRVARIAMIIGALF
                     TVVTLAALYFAPLDTQALHGDAPWRAVAPPGSLPSILAGVSNGIGTLLIVGGALYSAW
                     VFWRKGIMRSRMIGCILLAVGTLVVASGGILAGPLGQHAWLYPPMAVGVAIMFVGYLQ
                     TTRAPAPKPAAQPS"
     gene            4426..>4684
                     /locus_tag="VF276_03770"
     CDS             4426..>4684
                     /locus_tag="VF276_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019493779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6606012.1"
                     /translation="MTEPSRAPASARRTLRRIGLFSLTLLVVEFLDEFVFGAREAAWP
                     LIRDDLGLSYAQVGVLLGLPNIVGNLVEPVLGILGDVWRRRV"
BASE COUNT          687 a         1581 c         1575 g          841 t
ORIGIN      
        1 cgcgtatttg accacgctga acgcgagcgc cgaggtcgcc aggatggccg agaggcccag
       61 cgccgccgcc acgacatgca cgagcccgcc aagcgccgtg ccgagcgccg aggccagccc
      121 gacccgccgc ccgccttgca ggctgcgcgc caggacgtag aaaatgcccg gccccggcgt
      181 caacgccagg atcacggcgg cggtcaggaa cacggcaaag gcagcggaat cgggcatcaa
      241 ggcttcctca cataaactcg gatttcattc gtcgggcaaa ccggtttagt atagcgcggc
      301 gattggtcca tcggcaaggt ccaatcccgc gcaaatggga ggtgccattc cacggcccgg
      361 caagtagccg cttgcgtggc gcttgccggg ccgggttccc tcgatccgct tacttcgcgg
      421 gcagcagctc gttcgtgaac atggctgcgt agtccaggct ggtgaccttc tggtggttgg
      481 cgtcctccag gcggcccgtg tcgagcatga acttggggta gccgctccag acctgcaacg
      541 tctggtcgcc ccagcgcggc gcctcggcct gatactcctt ggcgagatac tgcgcgctct
      601 catgcaccag cgcggcgtcg gggaagctgc cgggcgggtt gcccgcgatc agcagatcgg
      661 cagcctggtc cggctgctgg atggcgaact cgaagccgcg cgccgtggcc gccatgaagc
      721 gccgccccac gtcggccttg tccttgagga acgcctcgct ggtgatgatc accgggctgt
      781 agtagtcggg cacgcagaag tcgcgtacca ggaactcgtt cagatcgacc ttgtcgtgct
      841 tggcctgcaa cgcctcccag cccttgtaga tccagacgaa atcggcttgc ttggcgacga
      901 gcgcctggta gccgcccgtg ttcgtggtga tgttctggat cggcgtggcc gggccgccgt
      961 cgcacttgat cacatcggcg atcaccggct cctcgtaggg cgtgccgaag ccggcgtagc
     1021 gcttgcccgc cagcttggcc gggcggtcca ggcccgagct tttcagcgtc gccagttccg
     1081 acgtattgtg ctggacgatg gcggcgacgg atttaaccgg caggcccgtc acccggtcga
     1141 tcaccacctg ctcgacaaag ctgatggcga aatcggcctt cccggcggcc accagggcgt
     1201 ccgggctggc cgcgtcggag tagggcagga tctccaggtc gatgccctgc tccttgtacc
     1261 agcccttttg ttgcgcgaca tagaatccgg tatggttggt gttcggcgtc cagtcgagcg
     1321 ccaggctcac cttttgcacg gggccggtat aggtggtggg ggccgccgct gcgttggtgc
     1381 ctgtcgcggg cgccgcctcg gtcgtcgtgg gcgcggcagc agtggccgtg gccgccgccg
     1441 tgggcatcgg cgccgaggta gaggtcgctg cctcggtagg cgccgtggcg ggcgctgatg
     1501 tcgaggtggc cgcctcgcct ccgcacgcgc tcaacagcag ggccgcgctc agcaccgcgg
     1561 cggcaagtac acgtttgaaa tacatatccc tgtaatctcc ttactcaaat accggtcact
     1621 ctgagcgccg cgaagagtct cgtctccgtt cccaacgaga cggagattct tcgctccgct
     1681 cagaatttct tgttttgact aattcttaca atttttggag attagctctg gttctagcag
     1741 caattcgaga caacactgtt aactaatgaa gtgatcgatt gctactgagt caagcccctt
     1801 tgtgaaaatt agtcaaaaca acaatcagaa tgacggattc ggattgacgg accaatgaga
     1861 ggaacccacg tatgatcgcc ggactccgtg gccggttggc ggggaaggac gccgatagcg
     1921 tcgtcatcga ctgcggcggg gtgctctacc gggtcttcgt gcccggctcg gccctgagcg
     1981 agctgagcgc cgacggcgcc gaggtgctgc tgcacacgca tctcgccgtg cgcgaagact
     2041 ccatgaccct ctacggctgc accacgcgcc gccagttgga cctgttcgag accttgctca
     2101 atgtgacggg tgttggcccc aaggtggccc tggcgatcat cggcagcatg cccgccgacg
     2161 ccctggagca ggccatcgcc cagggcgacc tggtgctgct gacgcgcgtg cccggcgtgg
     2221 gcaagaagct ggctcagcgc ctggtgctcg aactcaaagg caagctcgat ctggccgcgc
     2281 tcggcgtggg cacgcccggc gccgtgcggc ccaccggccc ccaggccgag gtggccgagg
     2341 ccctcggcgc gctcggctac aacgcgagcg aagtccagca ggtgctgccc aagctgccgc
     2401 aggacggctc cgtgcccgtc gaagaccttg tcatgcaggc gttgcagcag atgggccgct
     2461 agccgccgca gctactcgca gcagatggga ttgttcacta tggaagacct ggaactgagc
     2521 gcggtcgagg ccgcgtatgc gcgcctgcgc gaggtcgtgc gccgcacgcc cgtactcgct
     2581 tggggcgtga ccggcggcgc cggctggcag ccgcccgccg ggcagcgcct gaccctgaag
     2641 ctggaacatc tgcaactgac cggctcgttc aaggtacgcg gcgcctggaa cctcatcgcc
     2701 gatctcccgg cggagcagcg ggcgcgcggg ctggtgacgg cgtcgggcgg caaccacggg
     2761 ctggccgtgg cctgggccgc cgcgcgagcg ggcgtggccg ccacggtctt cctgccgcag
     2821 atgacccccg ccgcacagga agccaagatc cgcagcttcg gcgccgccgt cttccgccac
     2881 ggcctggcct gggacgacgc ctgggagggc gccgtggcct acgccgccga gcgcgacctg
     2941 ccgctggtgc atcccttcga cgacccgcgg attgtcgccg ggcagggcac ggtcggcctg
     3001 gaactgctgg agcaggcgcc cgatctcgat ctgctggtcg tcgccgtggg cgggggcggg
     3061 ctgctgggcg gcatcgccgc ctatgccggg cagcgccggc ccgatctgcg catcgtcggc
     3121 gtcgagccca ccggcgcggc cagtatgagc gccgcgctgc gagccgggga gccgctggcc
     3181 ctgcccggcg tgcagaccat cgcccatacc ctggccccgc gccgcgtgag ccggctgacc
     3241 ctggatctct gccgccgcta cgtcgaatcg gtggtgctgg tcgaggacgc cgacctggtg
     3301 gcggccatgg aacggctctg ggccgacgcg aacctgctcg tggagccgag cggcgccgcc
     3361 acgctggccg cgctgatggc cgggcgcata cccggcgcgg aaaccgccca ccatgtgggc
     3421 atcgtcgtct gcggcgccaa cgtcgatgcc gccccggcca ttgccctggc gacgggccgt
     3481 cacggctgag ccggtatatc gctataagat ttaatccaac attaagctac gccgccttgc
     3541 cgccctggga tcaacgtggt acactgaaca ccataccgga gcctgacgag gcgctttgca
     3601 gcactctttt tctcccctac tgcggcctgg ttctgaaagg attttgctat ggtgctgatg
     3661 ccgttgttcg ccatgatcgt gggttttgcc ctggcgatcg tcatcctgaa ccactatgcc
     3721 gccaacccgg cccgccgcac gcacgagttc atttggggta tttccttctt cctgttcgga
     3781 ctcggcgcgc tggccgaagt ggcggcggat ttcctgggct ggaccgacac aatggcccgc
     3841 gtctactaca tcagcggcgc gatcctggcc gtggcctacc tggggctggg cgaagcggtg
     3901 ctgctctggc cgggccgggt cgcccgtatc gcgatgatca tcggcgcgct gtttaccgtc
     3961 gtgaccctgg cggccctcta tttcgccccg ctggatactc aggcattgca tggcgatgcg
     4021 ccctggcgcg ctgtggcgcc cccaggctcg ctgccctcca tcctggccgg cgtcagcaac
     4081 ggcatcggca ccctgctcat cgtcggcggc gcgctctatt cggcctgggt cttctggcgc
     4141 aagggcatca tgcgtagccg catgatcggt tgcattttgc tggccgtggg tacgctcgtg
     4201 gtggcgagcg ggggtattct cgccgggccg ttgggccagc acgcctggct ctatccgccg
     4261 atggccgtgg gcgtggcgat catgttcgtg ggctatttgc aaaccacgcg cgcgccggca
     4321 cctaagcccg ccgcccaacc ttcgtaggtt ccctgctgcc atgatcagcc ccgccgggcg
     4381 gcaccacccg gcggggccgt tttgtgccct gggtaggagc accctgtgac cgaaccctcg
     4441 cgcgccccgg cctccgcgcg ccgcaccctg cgccggatcg gcctgttcag cctgaccctg
     4501 ctcgtggtcg agttcctgga tgagttcgtg ttcggcgcgc gcgaagccgc ctggccgctg
     4561 atccgcgacg acctgggcct cagctatgcc caggtgggcg tgctgctggg cctgcccaat
     4621 atcgtcggca acctggtcga gccggtgctg ggcatcctgg gcgatgtctg gagacgccgg
     4681 gtgc
//
LOCUS       DASUYC010000229         2433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_792108,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000229 DASUYC010000000
VERSION     DASUYC010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792108"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            606..1547
                     /locus_tag="VF276_03775"
     CDS             606..1547
                     /locus_tag="VF276_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX6606013.1"
                     /translation="MSSLTGPRPTVAVSEAVQTPARRRDWQKLAIIVAFLLPPGLIYF
                     LLVLLPVIQAVHFSFYRWNGLGPMEDFTGLDNYARILRDTVFRRALGNNLLIVGLSVV
                     VQLPLALGLALLIRRNLPGRAIFRVIFFLPYVLSEVITGVLWSFVYNPQYGLLNKVLG
                     VFPGFVPQGWLGDPKMVIYALFVVISWKYFGLHLILYMAGLQNVPAELEESAIIDGAN
                     GGQVIRHVTIPLLAPTIRLSLFLSVLGSLQIFDLVWVMTTGGPVNSSETMATYLYRYG
                     FQRFSIGYGSAVAVVLFAICFAFSFVYQRYVMRRDYQ"
     gene            1555..2412
                     /locus_tag="VF276_03780"
     CDS             1555..2412
                     /locus_tag="VF276_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015661936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX6606014.1"
                     /translation="MSSIPASQRRPGESLPLQLVKYGICIFIALLVLVPLVTAVVGGF
                     KTNADLQTRPFGWPDPLVLTNYSSVIANKAFWEELGNSTLVMLATTAGVVALASMAAF
                     VFARIRFAGREVLFNFFTLGLLFPLTVAILPLYITLRQANLVDNLWGIILPQVAFGLP
                     GNILILRGFFAAVPTELEEAAAIDGCTYFGFFWRVLLPLVRPSLAAVAVLTMVASWNN
                     YFLPLLVLNTASRYTLPLGIMQYQGQYGTDWARVLAFVSLALLPTLVFYLLAERHIVA
                     GLTAGAVKG"
BASE COUNT          341 a          818 c          805 g          469 t
ORIGIN      
        1 ggcgccgttc gcgcggcgct ccgccgggag ggaccagacc cccgctgcgc ccccggtgga
       61 ccctggtacg cacgccgtca cgctgcgcgc cgcgccgaga ctcttcgctg cgctcagagt
      121 gacaagcctg ggagggccgt ccttgggggg aacggtcatt ctgagcgcag cgaagaatct
      181 ccgtcggggg aacggtcatt ctgagcgcag cgaagaatct ccgtcttgtt ggggggtagc
      241 gccgagactc ttcgctgcgc tcagagtgac aaggcggggc gcgggggcgg gcaaggcggg
      301 ttgcaaaccc gcccctacac cgcgctcaga gtgacaaggc ggggcgcgtg ggggcggcaa
      361 ggcgggtttg aaacccgccc ctacaccgcg ctcagagtga caaggcgggg cgcgtgggcg
      421 gcaaggcggg tttcaaaccc gcccctacac cggcatcccg gcctgtttca gcgcctatcg
      481 tgggccgcag gcccatgatt tgctcagagt gacgactcgc tctcgcattt ctgacggctg
      541 atggctgata gctgacggct actgaccact gaccaccgac cactgaccac tcgtggagga
      601 tgccgatgag ctcgctgact ggaccccgcc ccacggtggc tgtaagcgag gcggtgcaga
      661 cgcccgcccg gcggcgcgac tggcaaaagc tcgccatcat cgtcgccttc ttgctgccgc
      721 cggggctgat ctacttcctg ctggtgctgc tgccggtgat ccaggcggtg catttcagct
      781 tctaccgctg gaacggcctg gggccgatgg aggacttcac cggcctcgac aactacgcgc
      841 gcatcctgcg cgacacggtg ttccggcgcg cgctgggcaa caacctgctg atcgtggggc
      901 tgtcggtggt ggtgcagttg ccgctggcgc tgggcctggc gctgctcatc cggcgcaacc
      961 tgccgggccg cgccatcttc cgggtcatct tcttcctgcc ctacgtgctc tccgaagtga
     1021 tcaccggcgt gctctggtcg ttcgtctaca atccgcagta tggcctgctc aacaaggtgc
     1081 tgggcgtgtt ccccggcttc gtgccgcagg gctggctggg cgaccccaag atggtcatct
     1141 acgcgctgtt cgtggtgatc agttggaagt acttcgggct gcacctgatc ctctatatgg
     1201 ccgggctgca aaacgtgccc gccgagctgg aggagtcggc catcatcgac ggcgcgaacg
     1261 gcggccaggt gatccgccac gtcacgatcc cgctgctggc gccgaccatc cgcctgtcgc
     1321 tgttcctgtc ggtgctcggc tcgttgcaga tcttcgacct ggtctgggtc atgaccaccg
     1381 gcgggccggt gaactccagc gagacgatgg cgacctacct ctaccgctac gggttccagc
     1441 gcttctccat cggctacggc agcgcggtgg cggtggtgct gttcgccatc tgctttgcgt
     1501 tctcgttcgt ctaccagcgc tacgtcatgc ggcgcgacta ccagtgaggt tgccatgtcc
     1561 agtatccctg cttcccagcg gcgccccggc gagagtctgc cgctccaact ggtcaagtac
     1621 ggcatctgca tcttcatcgc actgctggtg ctggtgccgc tcgtcaccgc ggtggtgggc
     1681 ggcttcaaga ccaatgccga cctgcaaacg cggcccttcg gctggcccga cccgctcgtc
     1741 ctgaccaact acagctcggt catcgccaac aaggcgttct gggaggagct cggcaacagc
     1801 acgctggtga tgctggcgac gacggcgggc gtggtcgcgc tggcgagcat ggcggccttc
     1861 gtcttcgccc gcatccgctt cgcggggcgc gaggtgctgt tcaacttctt cacgctgggg
     1921 ctgctgttcc cgctgaccgt ggcgatcctg ccgctgtaca tcacgctgcg ccaggccaac
     1981 ctggtcgata acctgtgggg catcattctg ccgcaggtgg ccttcgggct gccgggcaac
     2041 atcctgatcc tgcgcggctt cttcgcggcg gtgcccaccg agttggagga ggcggcggcc
     2101 atcgacggct gcacctattt cggcttcttc tggcgggtgc tgctgccgct ggtgcgtccc
     2161 tcgctggcgg cggtggccgt gctgaccatg gtcgccagtt ggaacaacta cttcctgccg
     2221 ctgctggtgc tgaacacggc ctcgcgctac acgctgccgc tgggcatcat gcaataccag
     2281 gggcagtacg gcaccgactg ggcgcgcgtg ctggccttcg tgtcgctggc gctgctgccc
     2341 acgctcgtct tctacctgct ggccgagcgc cacatcgtag ccggcctcac cgccggggcc
     2401 gtcaagggct aatcacccgg cagcctggcg tca
//
LOCUS       DASUYC010000230         4466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_971542,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000230 DASUYC010000000
VERSION     DASUYC010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971542"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /locus_tag="VF276_03785"
     CDS             complement(<1..1067)
                     /locus_tag="VF276_03785"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX6606015.1"
                     /translation="MTTTPASRPIPLNDTRPQYEAIGAEVRAAMDAVLERSWFILGQE
                     VAAFEREFAAFCGTAQAVGVSNGTDAIHIAVRALGLGPGDEVILPTHTATFSALGVSM
                     AGAVPRLVDVEPDTGNIDPAQIEAAITPATKAIMPVHLYGQAADMDPIRTVAARHGLA
                     VIEDAAQAHGATYRGATVGSLGRIGCFSFYPTKNLGAYGDGGAVTTDDADLAGIMREL
                     RHGGQADRYHHVRLGFCNRLDEIQAAVLRVKLPYLAGWNAARAERAAHYTRLLAAAGN
                     PVEPLAIRDYGQPVYHLYVVKTPNRRAELMAALKAAGIDSYIHDPVPVHLQEAYRFLG
                     HGPGSFPGAEDLAARIVSLPL"
     gene            complement(1079..2032)
                     /locus_tag="VF276_03790"
     CDS             complement(1079..2032)
                     /locus_tag="VF276_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX6606016.1"
                     /translation="MITGGLGFLGSNLARRLVEYGAEVLVIDSMIPEYGGNMFNVAGI
                     EDRLRININDVRLEHAMRYLVRGQEYIFNLAGQVSHLDSMTDPFTDLEINCRSQLSLL
                     EVCRKNNPAVKIVFAGTRQQYGKPDYLPVDERHLMHPTDVNGINKMAGEWYHILYNNV
                     YGVRACSLRLTNCYGPRQYVRDARMGFIGWFIHLAVEGKEISIYEPGTQLRDFNYVDD
                     VVDAFLLAGATDETNGQVYNLGGPPISHVDLTRMIIEIAGRGSYKLVPFPPERKAIDI
                     GDVYSDYRKIQAALGWEPRVPLCEGLEQTIAYYRKYHQYYW"
     gene            complement(2086..2985)
                     /locus_tag="VF276_03795"
     CDS             complement(2086..2985)
                     /locus_tag="VF276_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX6606017.1"
                     /translation="MGSNPETDTQTIAGNGPTTETKANNRAAAAVPWAEQPGILPAVD
                     EAGKPLPSAPGISVFFPAYNDGGTIASMVLEALRTCARVTNDYEVIVIDDASTDYTPE
                     VLDDLAAQNQHVRVIHHKQNAGYGGALRSGFQAASKELVFYTDGDAQYDPRELVVLLS
                     RMGPRVDIVQGYKIERHDPIFRIIIGRVYHWGVKLLFGLRVRDTDCDFRLIRKTALDR
                     IELESTSGTICVELVKKLQDSGARFAQVPVHHYHRVHGRSQFFNFPRLWRTATQLLQL
                     WWKLVVQGNKRSVAATSRRAQGK"
     gene            3375..4268
                     /locus_tag="VF276_03800"
     CDS             3375..4268
                     /locus_tag="VF276_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018533782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX6606018.1"
                     /translation="MPVETLPLVEQVRSFNRIMTERIGVLNDHFLGRDHPLGEARLIW
                     EIGEQGATVRALRRRLGLDSAYVSRLLRSLEGQGLVSVVPSGTDGRVRQVFLTAAGLR
                     ERAELDRRADAFAHSLLEPLSERERAKLAAAMAQVERLLIASMVQIGVADPTSADARW
                     CFEQYFAELGDRFEAGFDPVRSISASAHELTPPNGLLLIARLRDEPVGCAALKLHAQA
                     PAEIKRMWVAKRARGLGLGRRLLLELERHARELGVTVLHLETNRTLNEAIHLYRESGY
                     QEVAPFNDEPYAHHWFEKRLS"
BASE COUNT          705 a         1435 c         1509 g          817 t
ORIGIN      
        1 tagagcggca ggctgacgat gcgcgcggcc agatcctcgg cccccgggaa gctacccggc
       61 ccgtggccca ggaagcgata ggcttcctgc aaatgcaccg gcaccgggtc gtggatgtag
      121 ctgtcgatgc ccgcggcttt cagcgcggcc atcagttccg cgcggcggtt gggcgtcttg
      181 accacgtaca ggtggtagac cggctggccg tagtcgcgga tggcgagcgg ctcgaccggg
      241 ttgcccgccg ccgccagcag ccgcgtgtag tgggcggcgc gctcggcgcg ggcggcgttc
      301 cagcccgcca ggtagggcag cttgacgcgc agcaccgccg cctggatctc gtccagccgg
      361 ttgcagaagc ccaggcgcac gtgatggtag cggtcggcct ggccgccgtg gcgcaactcg
      421 cgcatgatcc cggccagatc ggcgtcgtcc gtggtcacgg cgccgccgtc gccgtaggcg
      481 cccaggttct tggtcgggta gaagctgaag cagccgatgc gccccaggct gccgaccgtc
      541 gcgccgcgat aggtggcgcc gtgcgcctgc gccgcatcct cgatcaccgc cagcccgtgc
      601 cgcgcggcga cggtgcggat cgggtccatg tcggcggcct ggccgtagag gtggaccggc
      661 atgatcgcct tcgtcgccgg ggtgatcgcc gcctcgatct gcgccgggtc gatgttgccg
      721 gtgtcgggct cgacatcaac cagccgcggc acggcccccg ccatcgacac gcccagggcg
      781 ctgaaagtgg ccgtgtgcgt cggcaggatc acttcgtcgc cggggccgag gcccagcgcc
      841 cgcacggcga tgtggatcgc gtcggtgccg ttgctcacgc ccacggcctg cgccgtgccg
      901 cagaaggcgg cgaactcgcg ctcgaacgcg gccacttcct ggccgaggat gaaccaactg
      961 cgctccagca ccgcgtccat ggccgcgcgc acttcggcgc cgatggcctc gtactgcgga
     1021 cgggtgtcgt tcagggggat gggccgcgag gccggagtcg tcgtcatggg aagatcctct
     1081 accagtaata ttggtgatac ttgcgataat aggcgatggt ctgttccagc ccctcgcaca
     1141 ggggcacgcg cggctcccag cccagggccg cctggatctt gcgataatcg ctgtacacgt
     1201 cgccgatgtc gatggccttg cgctccggtg ggaagggcac cagcttgtag gagccgcgcc
     1261 ccgcgatctc gatgatcatg cgggtgagat cgacatgcga gatgggcggc ccgcccaggt
     1321 tatacacctg cccgttggtc tcgtcggtgg ccccggccag caggaaggcg tccaccacgt
     1381 cgtccacgta gttgaagtcg cgcaactgcg tgcccggctc gtagatgctg atctccttgc
     1441 cctcgacggc caggtggatg aaccagccga tgaagcccat gcgcgcgtca cgcacgtatt
     1501 ggcgcgggcc gtagcagttg gtcagccgca gcgaacaggc gcggacgccg tagacgttgt
     1561 tgtagagaat atgataccac tcgccggcca tcttgttgat gccgttgaca tcggtcgggt
     1621 gcatcaggtg gcgctcgtcc accggcagat agtcgggctt gccgtactgc tgccgcgtgc
     1681 ccgcgaagac gatcttcacg gcggggttgt tcttgcggca gacttcgagc agcgaaagct
     1741 ggctgcggca gttgatctcc aggtcggtga acgggtcggt catgctgtcg aggtggctga
     1801 cctgcccggc caggttgaag atatactcct gcccgcgcac caggtagcgc atggcgtgtt
     1861 ccaggcgcac gtcgttgatg ttgatgcgca gccggtcctc gatgcccgcg acgttgaaca
     1921 tgttgccgcc gtactcgggg atcatcgagt cgatcaccag cacctcggcg ccgtactcga
     1981 ccaggcggcg ggccaggttg ctgccgagga agcccaggcc gccggtgatc atgacccggc
     2041 gcccgcggta gatgtcgggg aggtggttca gaaccggcac cacgcttatt tcccctgcgc
     2101 gcgccgggac gtggcggcca cgctgcgctt attaccttgc accaccagct tccaccacaa
     2161 ctgcaacagt tgcgtggcgg tgcgccagag ccgggggaag ttgaagaact gcgagcgccc
     2221 atgtacgcgg tgatagtgat gaacgggcac ctgggcgaag cgcgccccgc tgtcctgcag
     2281 cttcttcacc agctccacgc agatggtgcc gctggtcgat tccagctcga tgcggtccag
     2341 ggcggtcttg cggatcaggc ggaagtcgca gtcggtgtcg cgcacgcgca ggccaaacag
     2401 caacttgacg ccccagtggt acacccgccc gatgatgatg cggaagatgg ggtcgtggcg
     2461 ctcgatcttg tagccctgca cgatatcgac gcgcggcccc atgcgcgaca gcagcaccac
     2521 cagttcgcgc gggtcgtact gcgcgtcgcc gtcggtgtag aagaccagtt ctttgctcgc
     2581 cgcctggaag ccggagcgga gcgcgccgcc gtagcccgcg ttctgcttgt ggtggataac
     2641 ccgcacatgc tggttctgcg cggccaggtc gtcgaggact tccggcgtgt agtccgtgct
     2701 ggcgtcgtcg atgacgatga cttcgtagtc gttggtcacg cgggcgcagg tgcgcagcgc
     2761 ctccagcacc atgctggcaa tcgtcccgcc gtcgttgtag gcggggaaga agaccgaaat
     2821 ccccggcgcc gaggggaggg gcttcccggc ctcgtccacg gcgggcaaga tgcccggttg
     2881 ttcggcccag ggcacggccg ccgcggcgcg attattcgct ttcgtttccg ttgtggggcc
     2941 gtttccggca atcgtttgcg tgtctgtctc gggattacta cccatacaat aaccgaatct
     3001 ccctgttggc gtgcctaccg cacttcgtgg acccgtcccc gcgcaggcta taacatgcgt
     3061 gctgattata ccataccgcc cccagcgcca caaattgccg cgcgcccgtg cgctgtaata
     3121 ttcctctcgc gccaggcgtc gtaacaggta cgaggatcca tgcacatgcg ccgcatgatc
     3181 ttcgctcctt cctgaacccg gcgtcgctct gcgcagcgac gccggttttt tatagagcaa
     3241 gccccctttt ggcgcaacct catatggccg gaccggctga tctgcttccc ggatctcttg
     3301 tattatccaa ttatctgact ataatcagat aatgcgaggt aactgccggg ctagttagga
     3361 ggtttgttat ggagatgccg gttgagacgc tgccactggt ggagcaggtc cgcagcttca
     3421 atcgaatcat gaccgagcgc atcggcgtgc taaacgacca cttcctgggg cgcgaccatc
     3481 cgcttggcga ggcgcggttg atctgggaga tcggcgaaca gggcgcgacg gtgcgtgcgc
     3541 tccggcgccg gttggggctt gattccgctt acgtcagccg cctgttgcgt tcgctcgaag
     3601 ggcaagggtt ggtcagtgtg gtgcccagcg ggaccgatgg tcgggtgcgc caggtctttc
     3661 tcaccgccgc cggcttgcgc gaacgcgcgg aactagaccg ccgcgccgat gcgtttgccc
     3721 attcgctcct ggagccgctg agtgagcggg agcgcgccaa gctggcggcg gccatggcgc
     3781 aggtcgagcg gctgctgatc gcgtccatgg tgcagatcgg cgtcgcggat cccacgagcg
     3841 cggatgcccg atggtgcttc gagcagtact tcgccgaact gggcgaccgc ttcgaggccg
     3901 ggttcgatcc cgtgcgcagc atttccgcat cggcgcacga actgacgccg ccgaacggcc
     3961 tgctgctcat agcccggctg cgcgatgagc cggtgggctg cgctgcgttg aagctgcacg
     4021 cccaggcgcc tgcggagatc aagcgcatgt gggtcgcgaa gcgcgcacgc ggtctcgggt
     4081 tgggccggcg gttgctgctg gaactggagc gccatgccag ggaactggga gtcacggtcc
     4141 tgcatctgga aacgaaccgc acgctgaatg aagccattca cctgtaccgc gagagcgggt
     4201 atcaggaagt tgcgcccttc aacgacgagc cgtatgccca ccactggttc gaaaagcgcc
     4261 tttcgtgatg ctcccgatcc cgcacccggc gtcgctctgc gccgcggcac cggtttttta
     4321 tgaatccaca ggcgataaac gaggcggcgc cgtgtgatcc agcgccgcct gcacggttat
     4381 cgttcccgcc acaccgggtc actcgataaa catcagctcc atctgcgggg cggagaggaa
     4441 ttcgcagccg tggtcggtga cgagca
//
LOCUS       DASUYC010000231         2491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1911581, whole genome shotgun sequence.
ACCESSION   DASUYC010000231 DASUYC010000000
VERSION     DASUYC010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1911581"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..964)
                     /locus_tag="VF276_03805"
     CDS             complement(17..964)
                     /locus_tag="VF276_03805"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX6606019.1"
                     /translation="MADRFLVLPDDSMIPLLDALAAARQTIDIYVFTLSNPDILAALR
                     EAVARGVGVRAVVDPAPSENAAAGHAAMRALNDANVQVRPRPSYFERVHAKSFVVDGA
                     QALVSSINYLDDWMHTRDHGVLTTNPVMVQGIRQTFAADWAATPDDSVPAPPLVLSPA
                     NSRATLAGLIDGAQTSLILEEEQITDEAMGDLLAARSNAGVAVQIVTNALQTKNRPCL
                     ERLQARAPQAQVRYSTQLWLHTKLVIADERRMLVGSVNLTPTSLDRRREVSMLVDDPA
                     AVARAVAVARDDFASGASEPPDPDTAPPRPADSPGGGKD"
     gene            complement(1148..2239)
                     /locus_tag="VF276_03810"
     CDS             complement(1148..2239)
                     /locus_tag="VF276_03810"
                     /inference="COORDINATES: protein motif:HMM:NF042532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protealysin inhibitor emfourin"
                     /protein_id="HEX6606020.1"
                     /translation="MPIHTRLRGLVLVAGLALLTGCNVISMQPAATPTPITPPTTTPV
                     PVSDPTAPFVTFQVSGGIAGLQRAVSVQAGGAATLSERAQVIGTAQVPPDRLAEIRAK
                     LDAAQFFTLQDRYDNGRVADDIYYTITATEDGQTKKVTVAEVGGKESTPQAVIDLITN
                     LRELEGTVRANLTPAPGAAPATDTPAPPAATDTPMPEVTDTPLPAPATDTPATSGPPA
                     TATPVAGTPEPPAATATAEAGTVNEGADLVIYRVRGGIAGLDTAMFIGRHGAVTFQSR
                     GQETGRAQLTAAQVGELIDLFDSSGFFTFQDRYAPAAAPADSQELSVTYNHGGQVKTV
                     TVATEGQPPAALQTILDRLAALQADLQKK"
BASE COUNT          348 a          777 c          933 g          433 t
ORIGIN      
        1 caaccgggcg cggtagttag tctttgccac cgccggggct gtcggcggga cgcggcgggg
       61 cggtgtcggg gtcgggcggt tcggaggccc cggatgcgaa atcgtcacgc gcgacggcga
      121 cggcgcgggc cacggcggcg ggatcgtcga ccagcatact gacctcgcgc cgcctgtcca
      181 ggctggtggg cgtgaggttc acgctgccca ccagcatccg gcgctcatcc gcgatgacca
      241 gcttggtgtg caaccagagc tgggtgctgt agcgcacctg ggcctgcggc gcccgcgcct
      301 gcaagcgctc caggcagggt cgattcttag tttgcagtgc gttcgtgacg atctgcacag
      361 caacgcccgc gttgctgcgc gccgccagga gatcgcccat ggcctcgtcg gtgatctgct
      421 cttcctccag gatgagcgag gtttgcgccc cgtcgatcag gccggccagc gtggcgcggc
      481 tgttggccgg gctgagcacc aggggcgggg ccggcaccga gtcgtcgggc gtggcggccc
      541 agtcggcggc gaaggtttgc cggatgccct gcaccatgac cgggttcgtg gtcagcacgc
      601 cgtggtcgcg ggtgtgcatc cagtcgtcca gatagttgat gctggagacg agggcctggg
      661 cgccatcgac cacaaagctc ttggcgtgga cgcgctcgaa ataggagggg cgcgggcgga
      721 cctggacatt ggcatcgttc agcgcgcgca tggcggcgtg cccggcggcg gcgttctcgc
      781 tgggcgccgg atcgaccacg gcgcgcaccc ctactccgcg ggctacggct tcgcgcaacg
      841 cggccaggat atcgggattg ctgagggtga agacgtagat gtcgatggtc tgccgcgccg
      901 ccgcgagagc atcgagcagg gggatcatgc tgtcgtcggg cagcaccagg aaccggtcgg
      961 ccataggggt ctctccttcg catacaacgc taaggccgag accgtcgcac atcgcgtgcc
     1021 aggctatgga gacccgctaa ttcaccgcga aggcgcgaag aacgcgaaga ataagactaa
     1081 gaaaaataat cttcgcgtcc ttcgcgcctt cgcggtgcac gtccgaggct tgccgccggg
     1141 cgcggggcta tttcttctgt aagtcggctt gcaacgcggc cagccggtcc aggatcgtct
     1201 ggagggcagc gggcggctgg ccctccgttg cgacggtcac ggtcttgacc tggccgccgt
     1261 ggttgtaggt cacgctcaat tcctggctgt ccgccggggc cgccgcgggc gcgtagcggt
     1321 cctggaaggt gaagaagccg ctgctgtcga acaggtcgat cagttcgccc acctgggcgg
     1381 cggtcagttg ggcgcggccc gtctcctgcc cgcgcgactg gaacgtgacg gcgccgtgcc
     1441 gcccgatgaa catggcggtg tccagcccgg cgatgccgcc gcgcacccgg tagataacca
     1501 ggtcggcgcc ctcgttcacg gtcccggcct ccgcggtggc cgtggcggcg ggaggctcag
     1561 gcgtcccggc caccggggtg gccgtggccg gcggcccgga tgtcgccggg gtgtccgtgg
     1621 cgggggcggg cagcggggtg tccgtgacct cgggcatcgg ggtgtcggtc gccgcgggcg
     1681 gggcgggcgt atcggtcgcc ggggccgcgc cgggcgccgg ggtgagattg gcgcggacag
     1741 tgccttccag ttcgcgcagg ttggtgatca ggtcgatcac ggcctgcggc gtgctctcct
     1801 tgccgccgac ctcggccacc gtgaccttct tggtctgccc atcctcggtc gcggtgatcg
     1861 tgtagtagat gtcgtcggcc acgcggccgt tatcgtagcg gtcttgcagg gtgaagaact
     1921 gcgccgcgtc gagcttggcg cggatctcgg ccagccggtc gggcggcact tgcgccgtgc
     1981 cgatcacttg cgcgcgttcg ctgagcgtgg ccgcgccgcc ggcctggacg ctcacggcgc
     2041 gctgcaagcc ggcgatgccc ccgctgacct ggaaggtcac gaagggcgcg gtggggtcgc
     2101 tcaccgggac cggagtcgtg gtgggcggcg tgatgggagt cggcgtggcg gcgggttgca
     2161 tgctgatgac gttgcatccg gtcagcaggg ccaggccggc aaccaggacc aggccgcgga
     2221 gtcgtgtgtg gattggcata cagtctttct ttagctatca gcagtcagct atcagctgtc
     2281 aggttacgga gcgacgggta atcgtcagtg gtcggtagtc ggtggctgag catattgccc
     2341 tagcagtgat aggacgtggc tacaggcctg taagttccgt ggagtcgggc cggttcgctt
     2401 ttattccagg ccactcacca gccatgtctg gcatgcgcac gcaccgcttg tcagtagcac
     2461 gccgatgaac aagccacgcg cgatccaggg a
//
LOCUS       DASUYC010000232         2908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_971002,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000232 DASUYC010000000
VERSION     DASUYC010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971002"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2908
                     /locus_tag="VF276_03815"
     CDS             <1..>2908
                     /locus_tag="VF276_03815"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6606021.1"
                     /translation="ESGAEFHVLTGLARPAAPAPWPPLGPAAVPDEQVRPWLLPAVWD
                     RLHHGRGAFLAELRPVVALFLRFTGIDYTRPTAGARLDTFIRWVQEVLARYEAALLQL
                     TIGEKGSYLYAAFGAPLAHPDEAARAAAAALELRTPPPSCSFIRGVQIGISQGQVWTG
                     AYGGQARRTYGVLGDAVNLAARLMQSAAPGGILVTQAVRQAAGPRFGWAAMPALRVKG
                     KAGAVPVYGLVAERTEAEQALREPGAGAPLVGRAAELAQLDAALAQAHAGQGRIVGIT
                     GEAGLGKSRLVLEAVRRARAAGLPIYAGTAQSYGTNTPYLAWHAVWHAFFDLDPDGTP
                     DEIALELEDKLAEIDPALLERLPLLGPALGLPLPDTPLTAALDPELRKASLEALLIAC
                     LRARAAETPLALVLEDCQWLDPLSHDLLEAVGRAIPALPVLVLLAYRPPELERLRAPQ
                     VETLPHFTAIRLAPFVPDEARRLVAARLGEDHGAAWPPDLVEQIAARGDGNPFYLEEL
                     INYLAESGVDPRDGAALAAVEWPASLQSLLLARIDQLTEQQRTVLKVASIVGRRFVQP
                     ELWGVHPDLGPPEPVAADLAELDRLDLTPLDTPEPDPAYLFKHQMTQEVTYSSLPRAL
                     RARLHEQFAAWLEAGSAVAGGPPPLDLLAYHYGQSSNTAKQREYYRRAGDAAAAAYAG
                     PAAIDYYTRLLAVCPPGEERAARLALGQVLTEWGEWEAAAAHYQAALAEAEAARDTAA
                     VGQAAHGLSKVRRYQAAWPEAETWLERARAAYEAASDAGAVSRVLSDLGDVYRALGDY
                     ERARSLQAESIARARAAGDLHGQADALHRLGVLREKQGEYAEATALYEESMVHRRALG
                     DRPGIALCLSELATVALDQGDYARSRALRAESLALQREVGARPGIAQMLNNLGNVLDI
                     QGDHEGARALYEEALTLGRELGEANLTALTLGNLGLIAQAQGDYAAARARLEEAQ"
BASE COUNT          396 a         1089 c         1065 g          358 t
ORIGIN      
        1 gagagcggcg ccgagttcca tgtgctgacc ggcctggctc ggcccgccgc ccccgcaccc
       61 tggccgccgc tgggcccggc ggcggtgccc gacgagcagg tgcgcccgtg gctgctgccc
      121 gccgtgtggg accggctgca ccatggccgg ggcgccttcc tggccgaact gcgcccggtg
      181 gtcgccctgt tcctgcgctt caccggcatc gactataccc gtcctacggc gggcgcccgg
      241 ctcgacacgt ttatccgctg ggtgcaggag gtgctggcgc gctacgaggc ggcgctgctg
      301 caattgacga tcggcgagaa gggcagctac ctgtacgccg cctttggcgc gccgctcgcc
      361 caccccgacg aggcggcccg cgcggcggcg gcggccctgg aactgcgcac gcccccgccc
      421 agttgcagct tcatccgcgg cgtgcagatc ggcatcagcc agggccaggt gtggaccggg
      481 gcctatggcg ggcaggcccg gcgcacctac ggcgtgctgg gcgacgcggt caacctggcc
      541 gcccggctga tgcaatcggc ggcgccgggc ggcatcctgg tgacgcaggc ggtgcgccag
      601 gccgccgggc cgcgcttcgg ctgggccgcc atgcccgccc tgcgcgtgaa gggcaaggcc
      661 ggcgccgtgc ccgtctacgg gctggtagca gagcgcacgg aggccgagca ggctctgcgc
      721 gaaccgggcg ccggggcgcc gctggtgggc cgggcggcgg aactggccca actcgacgcc
      781 gcgctggccc aggcgcacgc cgggcagggc cgcatcgtgg gcatcaccgg cgaggccggg
      841 ctgggcaaat cgcggctggt gctggaggcc gtgcgccggg cgcgcgccgc cgggctgccc
      901 atctacgcgg gcacggccca gtcctacggc accaatacgc cctacctggc ctggcacgcc
      961 gtctggcacg cctttttcga cctcgacccc gacggcacgc ccgacgagat cgcgctggag
     1021 ttggaagata agctggccga gatcgacccg gcgctgctcg aacgcctgcc gctgctcggc
     1081 ccggccctgg gcctgcccct gcccgacacc ccgctgacgg cggcgctcga ccccgagcta
     1141 cgcaaggcgt cgctggaggc actgctgatc gcctgcctgc gcgcccgcgc cgccgagacc
     1201 cccctggccc tggtgctcga agattgccag tggctcgacc cgctgtcgca cgacctgctg
     1261 gaggccgtgg gccgggctat ccccgcgctg ccggtgctgg tgctgctggc ctaccggccc
     1321 ccggaactgg agcgcttgcg ggcgccgcag gtcgagaccc tgccccactt caccgccatc
     1381 cgcctggcgc ccttcgtgcc cgacgaggcc cgccggttgg tggctgcccg gctcggcgag
     1441 gaccacggcg ccgcctggcc ccccgacctg gtcgagcaga tcgccgcccg cggcgacggc
     1501 aaccccttct acctggaaga actgatcaac tacctggccg agtcgggcgt cgacccgcgc
     1561 gacggggcgg cgctggcggc ggtggagtgg ccggccagcc tgcaaagcct gctgctggcg
     1621 cgcatcgacc agttgaccga gcagcagcgc accgtgctca aggtcgccag catcgtcggg
     1681 cggcgctttg tacaacccga gctctggggc gtccaccccg acctcggccc gccggagccg
     1741 gtggccgccg acctggccga actcgaccgg ctggacttga cgccgctcga cacgccggag
     1801 cccgacccgg cctacctgtt caagcaccag atgacgcagg aagtgaccta cagcagcctg
     1861 ccgcgcgccc tgcgcgcccg gctgcacgag cagttcgccg cctggctgga agcgggcagc
     1921 gccgtggccg gcggcccgcc gccgctcgac ctgctggcct accactatgg gcaaagcagc
     1981 aacacggcca agcagcggga atattaccgc agagccggcg atgccgccgc cgccgcctac
     2041 gccggacccg cagccataga ctactacacg cgcctgctgg ccgtctgccc gcccggcgag
     2101 gaacgcgccg cccggctggc gctgggccag gtgctgaccg agtggggcga gtgggaggct
     2161 gccgccgcgc actatcaggc cgcgctggcc gaagccgagg ccgccaggga taccgccgcc
     2221 gtggggcaag cggcccacgg gctaagcaag gtccggcgct accaggcggc gtggcccgag
     2281 gccgagacct ggctggagcg ggcgcgggcc gcctacgaag cggcgagcga cgcgggcgcg
     2341 gtgagccgcg tcttgagcga cctgggcgat gtctaccggg cgctaggcga ttacgagcgg
     2401 gcgcggtccc tgcaagccga gagcatcgcc cgcgcgcggg cggccggtga cctgcatggg
     2461 caggcggatg ccctgcaccg cctgggcgtc ttacgggaaa agcagggcga gtacgccgag
     2521 gccaccgcgc tgtacgagga gagcatggta caccggcgcg ccctgggcga ccggcccggc
     2581 atcgcgctct gcttatccga gctggcgacg gtcgccctcg accagggcga ctatgcgcgc
     2641 tcgcgcgccc tgcgggccga gagcctggcg ttgcagcggg aagtgggcgc ccgccccggc
     2701 atcgcgcaga tgctcaacaa cctgggcaat gtgctcgata tccagggcga tcacgaaggg
     2761 gcccgcgccc tatatgaaga agcgctgacc ctggggcggg agctaggcga ggcgaacctc
     2821 accgccctca ccctgggcaa tctcgggctg atcgcccagg cgcaggggga ctacgcggcg
     2881 gcgcgcgccc gcctggaaga agcccaga
//
LOCUS       DASUYC010000233         5605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_794397,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000233 DASUYC010000000
VERSION     DASUYC010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5605
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794397"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="VF276_03820"
     CDS             complement(<1..639)
                     /locus_tag="VF276_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX6606022.1"
                     /translation="MAATRKPKLAVWKFSSCDGCQLSLLDCEDELLAVAGAVDIANFP
                     EASRAVVKGPYDLSLVEGSITTPHDAERIREVRRVSRFLVTIGACATAGGIQALRNFK
                     DVQTLIPLVYARPEYISTLDRSTPIAAHVPVDFELRGCPINKAQLLEVLNAYLHGRKP
                     NVPPHSVCVECKLRGTVCVMVAHGTPCLGPITHAGCGALCPTYHRGCYGCFGP"
     gene            complement(641..1477)
                     /locus_tag="VF276_03825"
     CDS             complement(641..1477)
                     /locus_tag="VF276_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEX6606023.1"
                     /translation="MITTTSMPMLVQPYRVQRVRKEIPGTLTLDLAAPDAGGFAFAPG
                     QFNMLYVWGVGEVPISISGDPARPQTLTHTIRAVGPVSEALCRLPRGATVGVRGPYGN
                     HWPVEAAVGSDVVVAAGGIGLAPLRPVLYHLLAQRPAYGNIVLLYGARTPQDLLYTDE
                     LARWRGRFDLEVGVTVDKATDGWQGHVGTVTTLIPKARFDPARTVAFVCGPEIMMRFT
                     IRELQTRGVPDERIFISMERNMKCAIGLCGHCQLGPFFICKDGPVFAYSAIKEWFGKR
                     EV"
     gene            complement(1560..2018)
                     /locus_tag="VF276_03830"
     CDS             complement(1560..2018)
                     /locus_tag="VF276_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX6606024.1"
                     /translation="MIDTSEYVLGEQAFLKGLSPAHLMAIAECAHPVTFASGEYVFHE
                     GDPANHFYILTYGQVSLELHVPGRGSHVIQTVEKDEVLGWSWLFPPYRWHFDGRALTV
                     VRAISFNGMCLRAKCDADHDLGYELMQRFAQTMMGRLQATRLQLLDVYGG"
     gene            complement(2015..3196)
                     /locus_tag="VF276_03835"
     CDS             complement(2015..3196)
                     /locus_tag="VF276_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX6606025.1"
                     /translation="MVATTPHTSVAPALGVACVIERAAFSALLAALRARGYELIGPTV
                     RDNAIVYDRIAGVEDLPAGWTDEQAGGHYRLKRRADAALFGYSVGPQSWKQFLYPPVL
                     RLWRGTRTETGFTVTPETDDPPQRALIGVRACELHAIAIQDRVLLQGPYVDPAYAARR
                     EKLFIVAVNCGQAGGTCFCVSMQTGPQATFGYDLALTEVLDGARHYFVVEPGTERGAE
                     VLAEVPHTLANTAEEAAATAAVAAATAQMGRSLDTDGLKDLLYRNTENPRWDMVAERC
                     LNCANCTMVCPTCFCTTVEEVTDLTGEHAERQRRWDSCFTTDFSYIHGGSVRPTPKAR
                     YRQWMTHKLATWIDQFGTAGCVGCGRCITWCPVGIDITEEARAIRESEGVAPIPPGGR
                     Q"
     gene            complement(3487..4788)
                     /locus_tag="VF276_03840"
     CDS             complement(3487..4788)
                     /locus_tag="VF276_03840"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX6606026.1"
                     /translation="MTTKLSPFSRDQLEAISRTYPTPFHIYDEAGIRASARRLRQAFA
                     WAPGFKEYFAVKATPNPYILTLLRAEGLGVDCSSLAELVLAQRCGFAGEDIMFTSNDT
                     PAHEFRQARALGAIINLDDLSHLRYLADHAGLPDLLCFRYNPGELRTGNAIIGNPKEA
                     KYGLTRAQLFEGYRRARDLGVRRFGLHTMVASNELDPQYFIDTAVMLFDLVGELSRAL
                     DIRFEFVNLGGGIGIPYKPEQQPVDLARVGAGVRAAYETRIAATGLHPLRLAMECGRV
                     ITGPSGYLVTRALHVKQTYKTFVGVDASMADLMRPGMYGAYHHITVPGKDGQPAAQVY
                     DVTGSLCENNDKFAVDRPLPEIAPGDLLVIHDTGAHGHAMGFNYNGKLRAAELLWRPD
                     HTVMLIRRAETLDDYFATLDFAGLPGFAAGGALAEPASRTP"
     gene            4947..5462
                     /locus_tag="VF276_03845"
     CDS             4947..5462
                     /locus_tag="VF276_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007379310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606027.1"
                     /translation="MILPKDRDPRFITIRRGGTLTDADHHLLALWAAACAEHVLPLFE
                     SVQPADSRPRHAIEQARAWVRGEIPMSQSRAAGGHAMAAARDLSGAPRHAAYAAGQAA
                     VVAHVAAHELGAAAYAIKAARAAAPEGEGERAGRRECRWQRDQLPEAIRDLVLDDQRL
                     RNDICWSVFDC"
BASE COUNT          938 a         1826 c         1937 g          904 t
ORIGIN      
        1 cgggccgaag cagccgtagc agccccggtg gtacgtcggg cacagggcac cgcacccggc
       61 gtgggtgatc ggcccgaggc acggcgtgcc gtgcgccacc atgacgcaga cggtgccgcg
      121 gagcttgcac tccacgcaga cgctatgcgg cggaacgttg ggtttgcgcc cgtgcaaata
      181 ggcgttcagc acctccagca gttgcgcctt attgatgggg cagccgcgca actcgaagtc
      241 gaccggcaca tgggcggcga tgggcgtgga ccggtccagc gtggagatgt actccgggcg
      301 ggcgtagacc agcgggatca gcgtctgcac gtccttgaag ttacgcagcg cctggatgcc
      361 acccgccgtc gcgcaggcgc cgatggtgac caggaagcgc gacacgcgcc gtacttcccg
      421 gatgcgctcg gcgtcgtgcg gcgtcgtgat cgacccctcc acgagcgaga gatcgtacgg
      481 gcctttcacc acggcccgcg acgcctcggg gaagttggca atgtccaccg cgcccgccac
      541 cgcgagcagt tcatcctcac agtcgagcag actcaactgg cagccgtcgc aggatgagaa
      601 tttccagacg gccagtttcg gtttgcgtgt cgccgccata tcaaacctct cgcttcccga
      661 accactcttt gattgccgag taggcgaaga cgggtccgtc tttgcagatg aagaacggcc
      721 caagctggca atggccgcac agcccgatgg cgcacttcat gttgcgctcc atcgagataa
      781 agatgcgttc gtcggggacg ccgcgggtct gcagttcgcg gatggtgaag cgcatcatga
      841 tctcggggcc gcaaacgaag gccacggtgc gcgccgggtc gaacctggcc ttggggatca
      901 gcgtcgtcac cgtgcccacg tggccctgcc agccgtcggt ggccttatcg acggtaacac
      961 ccacctccag gtcgaagcgc ccgcgccagc gcgccagttc gtcggtatac agcaggtctt
     1021 gtggggtgcg cgcgccgtag agcagcacaa tgttgccgta ggcggggcgc tgggccagca
     1081 ggtgatagag cacggggcgc aagggcgcca ggccgatccc gcccgcggcc accaccacat
     1141 cgctgcccac cgcggcttca acgggccagt ggttgccgta gggcccgcgc acgcccaccg
     1201 tggcgccgcg cggcagccgg cacagcgcct ccgagaccgg accgactgca cggatcgtat
     1261 gcgtgagcgt ctgcggccgg gccgggtccc cgctgatcga aatgggcact tcgcccacgc
     1321 cccagacata cagcatgttg aactggccgg gcgcgaaggc gaagccgccg gcatccggcg
     1381 cggcgagatc gagcgtcagg gtgccgggga tctccttgcg cacccgttgc acgcgatagg
     1441 gttggaccag catgggcatg ctcgtcgtcg tgatcatccc gctacctctc atagccatac
     1501 cgcggggggc tgccggaagc cggcgcccgc gcgggtgggg ttttgcggca ggtcgttacc
     1561 tacccgccat agacatccag cagttgcagg cgcgtcgcct ggagccggcc catcatggtc
     1621 tgggcaaacc gctgcatcaa ctcgtagccc aggtcgtggt cggcgtcaca cttggcgcgc
     1681 aggcacatcc cgttaaaaga gatggcgcgc accaccgtca gggcgcgccc gtcgaagtgc
     1741 cagcgatagg gcgggaacaa ccacgaccag ccgagcacct cgtccttctc caccgtctgg
     1801 atcacgtggg atccccggcc cggcacgtgg agttcgagcg acacctggcc gtaggtgagg
     1861 atatagaagt ggttggccgg atcgccctcg tggaacacgt attccccgct cgcgaacgtg
     1921 acgggatgcg cgcactcggc aatcgccatg aggtgggcgg gggagaggcc cttcaagaag
     1981 gcttgctcgc ccagcacata ctccgatgta tcgatcattg ccggcctcca gggggaatgg
     2041 gagcgactcc ctcagactcg cggatggcgc gggcctcttc ggtgatatcg atgccgaccg
     2101 ggcaccaggt gatgcagcgg ccgcaaccga cgcagcccgc cgtgccgaac tggtcgatcc
     2161 aggtggcaag cttgtgcgtc atccattgcc ggtagcgcgc cttgggcgtg ggtcgcacgc
     2221 tgccgccgtg gatgtaggag aagtcggtgg taaagcacga gtcccagcgg cgctgccgct
     2281 cagcgtgctc accggtcagg tccgtcactt cctccacggt ggtgcagaag caggtggggc
     2341 aaaccattgt gcagttggcg cagttcaggc agcgctcggc caccatatcc cagcgcgggt
     2401 tctcggtgtt gcggtagagc agatccttga ggccgtccgt gtccaggctg cgacccatct
     2461 gtgcggtcgc cgccgccacg gctgctgtag ccgcggcctc ttcagccgta ttggcgagcg
     2521 tatgcggcac ttcggcgagc acctcggccc cgcgctcggt gccgggctcg accacgaagt
     2581 agtgccgtgc cccgtcgagc acttccgtca aggccaggtc gtagccgaag gtggcctgcg
     2641 gtcccgtctg catcgagacg cagaaacagg tgccgcccgc ctggccgcag ttcacggcca
     2701 cgatgaacag cttctcccgc cgcgccgcat acgccggatc gacgtacggc ccctgcaaca
     2761 ggacgcggtc ctggatggcg atggcgtgca gttcgcacgc ccgcacgccg atcaacgcgc
     2821 gctgcggcgg atcgtcggtc tcgggcgtca cggtgaagcc ggtctcggtc cgtgtgcccc
     2881 gccacagccg cagcaccgga ggatagagaa actgcttcca ggattgcggc ccgacgctgt
     2941 agccgaacag ggcggcatcg gcgcgccgtt tgagccgata gtgcccgccc gcctgctcgt
     3001 cggtccaccc ggcgggcaaa tcctcgacgc cggcgatgcg gtcatagaca atggcgttgt
     3061 cgcgcacggt ggggccgatt aactcgtagc cgcgggcacg caaggccgcc aggagggcgg
     3121 agaatgcggc ccgctcgatg acgcaggcga cgccgagcgc cggagctaca ctcgtgtgcg
     3181 gagtagttgc gaccatggag aggggcctcc attgcatgga tacaggcaga gtcgtgtgcg
     3241 tgtggttcgc cgggggatgg ttccggccgg tcacgcgagg gcggcccatc acgcggccac
     3301 caatcccagt gtagcagcgg caggtcatag ccccggtaaa tgccggttgc agattcgtga
     3361 caggaacgcg ggtaggcagg atagatgtgg tgattgccgc gctacccagg agtcttcttg
     3421 ttcacggaac tactctcctg cctgccgtgc tgcgcggcaa ccggccgggc aggggcagct
     3481 gtccgctcag ggggtgcggg aggcaggttc cgcgagcgcg ccaccagcgg cgaagccggg
     3541 cagtcccgcg aaatcgagcg tggcgaaata gtcgtccagc gtttcggcgc gccggatgag
     3601 catgacggtg tgatcggggc gccacagcag ttccgccgcg cgcagcttgc cgttgtagtt
     3661 gaagcccatg gcgtgcccgt gcgcccccgt gtcgtggatc accagcagat cgcctggcgc
     3721 gatttccggc aggggccggt ccacggcaaa cttgtcgttg ttctcgcaga gcgacccggt
     3781 cacatcgtac acctgcgcgg cgggctggcc gtccttgccg gggacggtga tatggtggta
     3841 cgccccgtac atgccgggcc gcatcaagtc ggccatgctg gcgtccacgc ccacgaacgt
     3901 cttgtaggtc tgcttcacat gcagcgcgcg cgtcaccagg tagccggacg gtccggtgat
     3961 gacgcgcccg cattccatgg ccaggcgcaa gggatgcagc ccggtcgccg cgatccgggt
     4021 ctcgtaggcg gcgcggacac ccgcgccgac ccgcgccagg tccaccggct gctgctcagg
     4081 cttatagggg atgccgatac cgccgcccag gttcacgaac tcgaagcgga tatcgagcgc
     4141 ccgcgagagt tccccgacca ggtcgaacag catcaccgcc gtgtcgatga aatactgcgg
     4201 gtccagttca ttcgacgcga ccatggtgtg cagcccgaag cggcgcacgc ccaggtcgcg
     4261 ggcgcgccgg tagccctcga acaactgggc gcgggtcagc ccatacttgg cctctttcgg
     4321 gttgccgatg atggcgttgc cggtgcgcaa ctcgccgggg ttgtagcgga agcagagcag
     4381 gtcgggcagc ccggcatgat ccgccaggta gcgcaggtgc gacagatcgt ccaggttgat
     4441 gatcgcgccc agcgcccgcg cctgccggaa ctcgtgggcc ggggtgtcgt tcgaggtgaa
     4501 catgatgtcc tcgccggcaa acccgcagcg ctgggccaat accagttcgg ccagcgagct
     4561 acaatcgacg cccagcccct cggcgcgcag cagcgtgagg atatagggat tgggtgtggc
     4621 cttgacggcg aagtattcct tgaagccggg cgcccaggcg aaagcctgcc gcaatcggcg
     4681 cgcgctggcg cggatgcccg cctcgtcgta gatgtgaaac ggcgtggggt aggtgcggct
     4741 gatcgcttcg agttggtcgc gtgaaaacgg cgagagttta gtcgtcatta ctgtcctttg
     4801 caaagtcgtg tatttgcccg ccgccgggcc aggcctgctt tattatacac cggcgcagac
     4861 gccgcgcggg cggatcggtg tatactgagc ataccgtata gataaagcta aggaatgtta
     4921 aaatttctgc tcaggaaagg tgcagcatga tcctccccaa agatcgcgac ccgcgcttca
     4981 tcaccatccg ccgcggtggg acgctcacgg atgcagacca tcacctgctc gccttgtggg
     5041 cggctgcgtg tgcagagcac gtcctgcccc tcttcgagtc ggtgcagccg gcggactcgc
     5101 ggccacgtca cgccattgag caggcgcggg cctgggtgcg cggcgagatc ccgatgtccc
     5161 agtcccgcgc ggcgggcggc catgcgatgg cggcggccag ggatctgagc ggcgcaccgc
     5221 gccatgccgc gtatgcggcc gggcaggcgg cggtggtcgc ccacgtcgcc gcgcatgagc
     5281 tcggcgccgc cgcctacgca atcaaggccg cgcgcgcggc ggcgccggaa ggcgagggcg
     5341 agcgggccgg ccggcgcgag tgccgctggc agcgcgacca gttgccggag gcgattcgcg
     5401 atctggtcct ggacgatcag cggttgcgca acgacatctg ctggtcggta ttcgactgct
     5461 gatgggcgac gggcgtgtgc cggcacggcc caacaagcgc tgcgcaccgg caccgcgagc
     5521 ccgacatcct ggtgacgttg cagcctcgct agagccggcg caagccgcgg cacgcaaatg
     5581 gatgtatagt agctgaaagg cccgt
//
LOCUS       DASUYC010000234         2134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_416205,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000234 DASUYC010000000
VERSION     DASUYC010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416205"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /locus_tag="VF276_03850"
     CDS             <1..962
                     /locus_tag="VF276_03850"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEX6606028.1"
                     /translation="AFARLRLRPRILAGVGTRDLRTTLLGQPVSFPVLVAPMAFHQLV
                     CPDGELATARAAGAAGTIMCVSTAANYSLEDVAAAATGPLWFQLYCYKDRELTRHLVD
                     RARAAGYRALVLTADTPMVGRRERDIRNRFTLPPGTGWKNVEAYGKRDLPQDVEGSGL
                     AAYVAGQQDSNLTWADVDWLVGSSRLPVLVKGVLCAADARLAAEHGAAGVIVSNHGGR
                     QLDTALATIDALPEIAAAAADGNLTVLLDGGVRRGTDVLKALALGAQAVLLGRPVLWG
                     LAVGGEAGARTVLDALRAEFDLALALAGATSLAGLDADLVVRP"
     gene            959..1417
                     /locus_tag="VF276_03855"
     CDS             959..1417
                     /locus_tag="VF276_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606029.1"
                     /translation="MSTQRLPPGPGYPVPVEHPTQPLGPLRRYTRRRYMLFHLGFTLR
                     LVGRCLADPQVAAGRKLIFLGVLGVLMLALLVPETGADLIGLFVPIFDLFGIPFEGVV
                     DWGFFVFALGWLIGIFPAEVLRRHIAELQGRGWPAPPAPPPPPQLPPPRR"
     gene            1540..>2134
                     /locus_tag="VF276_03860"
     CDS             1540..>2134
                     /locus_tag="VF276_03860"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX6606030.1"
                     /translation="MTHAPASKEAPAGDSPSSPFAPPTPDPARNPIPAGRRTLVVGQA
                     GGPTPVINASLAGVVQAAQAAGFTRILGLRYGVRGLLGGQYADLTGLSGAELDRLART
                     PSSALGACRLKLDDAQVATGLAALRALEAGGFVYIGGNDSADTAHRLARAAAQADDPL
                     RVLAVPKTMDNDLPLTDHCPGYGSVARYVATSTLETTL"
BASE COUNT          258 a          813 c          754 g          309 t
ORIGIN      
        1 ccgcctttgc ccgcctgcgg ctgcgcccgc gcatcctggc gggcgtgggc acgcgcgacc
       61 tgcggaccac gctcctgggg cagcccgtca gcttcccggt gctcgtcgcg ccgatggcct
      121 tccaccagct cgtctgcccc gacggcgaac tcgccacggc ccgcgccgcg ggcgccgccg
      181 gcacgatcat gtgcgtcagc actgccgcga actacagcct ggaggatgtg gcggcggcgg
      241 cgaccgggcc gctctggttc cagctctatt gctacaagga ccgcgagttg acgcgccacc
      301 tcgtggaccg ggcgcgggcc gccggctatc gcgcgctggt gctcaccgcc gacacgccga
      361 tggtgggccg gcgcgagcgc gacatccgca accggttcac cctgccgccc ggcacgggct
      421 ggaaaaacgt cgaagcctac ggcaaacgcg atctaccaca ggatgtcgaa ggttcgggac
      481 tggcggccta cgtggccggc cagcaggaca gcaatctcac ctgggccgac gtggactggc
      541 tggtgggcag cagccggttg ccggtgctgg tcaagggcgt gctctgcgcg gcggacgcgc
      601 gcctggccgc cgaacacggc gcggcgggcg tgatcgtctc caatcacggc ggccggcagt
      661 tggacacggc tctcgccacc atcgacgccc tgccggagat cgcggcggca gccgcggacg
      721 gcaacctgac ggtgttgctc gacggcggcg tgcggcgcgg caccgacgtg ctcaaggcac
      781 tggccctggg cgcccaggcg gtgctgctgg ggcgcccggt gctctggggg ttggccgtgg
      841 gtggcgaggc gggagcgcgg acggtgctcg acgccctgcg cgccgagttc gatttggcgc
      901 tggccctcgc cggggccacg tccctggccg gcctggacgc ggacctggtg gtgcggccat
      961 gagcacgcag cgcctgccgc ccggccccgg ctatcccgtg ccggtggaac atcccacgca
     1021 gccactgggg ccgctgcgcc gctacacgcg ccggcgctac atgctgttcc acctgggctt
     1081 caccctgcgc ctggttgggc gctgcctggc cgatccccag gtggccgccg ggcgcaagct
     1141 catcttcctc ggcgtcctcg gcgttctgat gctggccctg ctggtgccgg agaccggcgc
     1201 cgacctgatc ggcctgttcg ttcccatctt cgacctgttc gggatcccgt tcgagggcgt
     1261 ggtggattgg ggcttcttcg tcttcgcgct cggctggctc atcggcatct tccccgcaga
     1321 ggtcttgcgt cggcacatcg cggaactcca gggccgcggg tggcccgccc cgcccgctcc
     1381 cccgccgccc ccgcaactcc cgccgccgcg ccggtagccc cgcccgccgg gcgggccgtt
     1441 gccttgacac tcgccgcggc ttgggcgtag aatcaggcgg cggccgcaac cggcggtccg
     1501 tcgcagatcc acacttggaa gaggagtatc agcaccccga tgacccatgc gcctgcctct
     1561 aaagaggcac ctgccggcga ttcgcccagt tcaccctttg cccccccgac tcccgatccc
     1621 gcgcgtaatc ccatcccggc gggccgccgc acgctcgtgg tgggccaggc aggcggcccc
     1681 acgccggtga tcaatgccag cctggccgga gtggtccagg ccgcccaggc cgccggcttc
     1741 acccgcatcc tgggcctgcg ctacggcgtg cgcggcctgc tcggcggcca gtacgccgac
     1801 ctgaccggcc tgagcggcgc ggagttggac cggctggccc gcacgccgtc gtcggcgctc
     1861 ggcgcctgcc ggctcaagct ggatgacgcg caggtggcga cgggcctggc cgcgctgcgc
     1921 gccctggaag cgggcggctt cgtctacatc ggcggcaacg actcggcgga cacggcgcat
     1981 cgcctggcgc gcgccgccgc gcaggcggac gacccgctgc gcgtgctggc cgtgcccaag
     2041 accatggaca acgacctgcc gctcaccgac cactgccccg gctacggcag cgtggcccgc
     2101 tacgtggcga ccagcaccct ggagacgacg ctcg
//
LOCUS       DASUYC010000235         3409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1894789, whole genome shotgun sequence.
ACCESSION   DASUYC010000235 DASUYC010000000
VERSION     DASUYC010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1894789"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="VF276_03865"
     CDS             <1..970
                     /locus_tag="VF276_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HEX6606031.1"
                     /translation="VKPDGAKVEDKATLDRIRSLAIPPAYTDVWICAKANGHIQATGR
                     DAKGRKQYRYHPAFREVRESTKYEHMLEFAQGLPAIRKTIDEHMSLRGLPREKVLATV
                     VHLLETTLIRVGNSDYVKQNKSYGLTTLRDPHVKVEGGELRFQFKGKSGKTWKLQVKD
                     RRIARIVKACQDLPGQDLFQYVDENGEQQAITSADVNAYLKEITGRDITAKDFRTWAG
                     TVLAAMALQEFEATETETQAKKNVVQAIEHVAERLGNTPAVCRKCYVHPAIIESYLDG
                     SMRAAVKQRVAAEAADPEDLHPDEAKVMGLLQARLKDEGAPAQKAG"
     gene            1120..2253
                     /locus_tag="VF276_03870"
     CDS             1120..2253
                     /locus_tag="VF276_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606032.1"
                     /translation="MPPAPNPSEIYCPRCGAANDAALPRCLLCGTPLTAPDRARAAVP
                     PVAPPVVPPPAYAPWPVQAAYPPAPAPPPAATPPGYHALQAPPYLPPGYVPHPPPGYV
                     PPAPPAPGPSPYAPAPYAGQPPPAVPLPHRSGLRIGVALGLLFSVCVITLLSGSALAV
                     IWGGSMAPVVAPPPPDSTLLLDQAADAHAQLKSVHYQLTAEFPSATGGSDLPRQIMMD
                     GDVIFPDSYTMHSSVYGERIVLGDTVYRRAAGSATWQRVGPDTPDQNAPVSATDLLTV
                     VKYYDTTDPAEEVISGTRRLVRVHFTVDTQRMAADEGPDAAVAALAGSDVQATALIDE
                     ATRYITRLTLRIVTPDNARGNFTLVLSRFDAPVTIKPPPNSSP"
     gene            complement(2550..>3409)
                     /locus_tag="VF276_03875"
     CDS             complement(2550..>3409)
                     /locus_tag="VF276_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606033.1"
                     /translation="STRPACCAGGARPRRTTAMPDARALAAFADFMLAAAPRLLLYPG
                     GLFAAACALVLALALPRITRTPPPPAGPPPAREGDLLAALALAWTAWALLPLPGAPDL
                     PGAPDLLGPLALLLGATLLARAHRPDVDWRREALGRPLLLGAPLVALVALGAPGLLPA
                     APGAVPFLAAMGRLLAALAYVVGFCVCYAAPGTAPWTARGPIVARAAGWMEWLGWLGL
                     AVALGPAERATAGGPAWAGPVLVAVIAAAAAAGTGTPVARALAQRGAPLGWLALGLAL
                     LLVLLAGQG"
BASE COUNT          555 a         1269 c         1174 g          411 t
ORIGIN      
        1 cgtcaaaccc gacggcgcga aggtggagga caaggccacc ctcgaccgca tcaggtcgct
       61 tgccattccg ccggcctata cggacgtatg gatctgcgcg aaggccaacg ggcatatcca
      121 ggcgaccggc cgcgatgcca agggccgcaa gcaataccgc taccatccgg cgttccgcga
      181 ggtccgcgag agcacgaagt acgagcacat gctcgaattc gcgcaaggcc tgccagcgat
      241 ccgcaagacc atcgacgagc atatgagcct gcgcgggctg ccgcgcgaga aggtgctggc
      301 gaccgtggtg cacctgctgg agacgacgct gatccgcgtc ggcaattccg actacgtgaa
      361 gcagaacaag agctacggcc ttaccacctt gcgcgatccg catgtgaagg tggaaggcgg
      421 cgagctgcgc ttccagttca agggcaagag cggcaagacc tggaaattgc aggtcaagga
      481 ccggcgcatc gcgaggatcg tcaaggcctg ccaggacctg ccgggccagg acctgttcca
      541 gtatgtcgac gagaacggcg agcagcaggc gatcacctcg gcggatgtga acgcctatct
      601 caaggagatc accggccgcg acatcacggc caaggatttc cgcacctggg ccgggacggt
      661 gctggcggcg atggcgttgc aggagttcga ggcgaccgag accgaaaccc aggccaagaa
      721 gaacgtggtg caggccatcg agcacgtggc cgagcggctg ggcaacacgc ccgccgtgtg
      781 ccgcaagtgc tacgtccacc cggcgatcat cgagtcctac ctcgacggct cgatgcgggc
      841 cgccgtgaag cagcgggtcg ccgccgaggc cgcggacccg gaggacctgc accccgacga
      901 ggccaaagtc atggggctgt tgcaggcgcg gctgaaggac gagggggcgc ccgcgcagaa
      961 ggcgggataa gctcgccgcg tcggcccggc aactggggac gaaagtcccc gcgcggcgcc
     1021 gccctgtccg atcttgcatc gtggcgtggc ccggtgttat gatagaccgg agagccgccc
     1081 cgcatgcccg gccgcggcct cgctggaaga ggtctcgcta tgccgcccgc gcccaaccca
     1141 tcggaaatct attgcccgcg ctgcggcgcc gccaacgacg cggcgctgcc ccggtgcctg
     1201 ctctgcggca cgccgctgac cgcgcccgac cgggcgcgcg ccgccgtccc gcccgtggcg
     1261 ccgccggtcg tgccgccgcc cgcctacgcg ccctggcctg tgcaggctgc gtatcccccg
     1321 gcccccgccc cgcctccggc ggccaccccg cccggctatc acgcgctgca ggcgccgccc
     1381 tatctgccgc ccggttatgt gccccatcca ccgcccggct acgtaccgcc ggccccgccc
     1441 gcgcccggcc cctcacccta tgccccggcg ccctacgccg gtcagccgcc gcccgccgtg
     1501 cccctgccgc accgcagcgg gttgcgcatt ggggtggcgc tggggttgct gttcagcgtc
     1561 tgcgtcatta ccctgctgag cggtagcgcc ctggccgtca tctggggcgg cagcatggcc
     1621 cccgtcgtcg ccccgccgcc gcccgattcg accttgctgc tggaccaggc cgccgacgct
     1681 cacgcccagc ttaagagcgt ccactaccag ctcacggcgg agtttcccag cgcgaccggc
     1741 ggcagcgacc tgccgcgcca gatcatgatg gacggcgatg tcatcttccc cgacagctac
     1801 accatgcaca gcagcgtcta tggcgaacgg attgtgctgg gggacaccgt ctaccgccgg
     1861 gcggcgggta gcgcgacctg gcagcgggtc ggccccgata cgcccgacca gaacgcgccg
     1921 gtcagcgcga ccgacctgct gaccgtggtc aaatactacg acacgacaga cccggcggag
     1981 gaggtcatca gcggcacgcg gcggctggtc cgcgtccatt tcaccgtgga cacccagcgc
     2041 atggccgccg acgaagggcc ggatgcggcg gtagccgccc tggccggctc cgatgtccag
     2101 gccaccgcgc tgatcgacga ggcgacccgc tacatcaccc ggctcaccct gcgcatcgtg
     2161 acgccggaca acgcgcgcgg caacttcacc ctggttctct cccgcttcga cgccccggtg
     2221 acgatcaagc ccccgccgaa cagcagcccg tagtcgtccc cccactgtga ttctgtaggc
     2281 ggcaggccgg tttaaaaccg gcccctatat cgcgcactta ttgggacccc ccgaccggtc
     2341 ccgattcccc ctggctgtag ccgcgcgatt tatcgcgccg tactcgttgg tcgctagcgt
     2401 gcgctctccc caacagattc ctggaactca tgcttgggca accaatactc tgttgcgttg
     2461 tagaggccgg tttcaaaccg gcccggcggt gccagatgcg ttgtaggggc cggtttcaaa
     2521 ccggcctgcc gccggatcgg gccggatggc tagccctgcc cggccagcag caccagcaac
     2581 aacgccaggc cgagcgccag ccagcccagc ggcgccccgc gctgcgccag ggcacgggcc
     2641 acgggcgtgc cggtcccggc ggcggcagca gcggcgatca ccgccaccag cacgggccca
     2701 gcccaggccg gaccgccggc ggtggcgcgc tccgccggac cgagcgccac ggccagcccc
     2761 agccagccca gccactccat ccagcccgcg gcgcgcgcca cgatggggcc gcgggcggtc
     2821 cagggcgccg tccccggcgc ggcatagcag acacagaacc ccacgacata ggccagcgcg
     2881 gccagcaagc ggcccatggc ggccaggaaa ggcaccgcgc ccggtgccgc gggcagcagc
     2941 cccggcgcgc cgagcgcgac cagagccacc aggggcgcgc ccaataaaag cgggcggccc
     3001 agcgcctcgc gccgccagtc cacgtccggg cgatgggcgc gcgccagcag agttgcccct
     3061 agcagcaggg cgagcgggcc gagcaggtcc ggcgcgccag gcaggtccgg ggcgccgggc
     3121 agcggcagga gcgcccacgc ggtccaggcc agagccagcg ccgccaagag atccccttcg
     3181 cgggcgggcg gcggcccagc gggcgggggc ggtgtgcgcg tgatgcgcgg cagggcgagg
     3241 gccagcacga gcgcgcaggc ggcggcgaac agcccgccgg gatagagcag cagccggggc
     3301 gcggcagcca gcatgaaatc ggcgaaggcg gccagggcgc gggcgtcagg catcgcggtg
     3361 gtccggcggg gacgggcgcc gccggcgcag caggcgggcc gggtcgatc
//
LOCUS       DASUYC010000236         3269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_2044957, whole genome shotgun sequence.
ACCESSION   DASUYC010000236 DASUYC010000000
VERSION     DASUYC010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2044957"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="VF276_03880"
     CDS             <1..642
                     /locus_tag="VF276_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606034.1"
                     /translation="EYYGDAERILRGHLLPSQLRDISFIAAPLDAGATDGTRDVARRH
                     RGAFGFPAPYGHEPVGMDHVSFNMDIVGGAVGSLCAAYRHIARRDPDGHRVNLLFDHE
                     TADLKVESPYTHPHLRVTVKRPAPLFVRLPAWVRPETIRIPGLAGPPQIVAGYLTIPD
                     PPVNQALSIEFPLPEAEIVLAHRTRQIRTRLRGDQVLAMDNFGADLTFFDSID"
     gene            700..2814
                     /locus_tag="VF276_03885"
     CDS             700..2814
                     /locus_tag="VF276_03885"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEX6606035.1"
                     /translation="MTLPNDYLERVYAGVLGKMIGVYLGRPFEGWTHERIMAELGPIQ
                     YYVHERLGVPLIVTDDDLSGTFTFLRALPDHGYRRDLTPAEIGETWLNYIIEGRTVLW
                     WGGLGNSTEHTAFLRLKAGIEPPASGSMALNSRVVAEQIGAQIFIDGWGMVAPGDPEL
                     AADLARRAACVSHDGEAIYGAQVLAAMEALAFVEPDLNRLLDTAVALIPRDSIIYRLI
                     ADIREWHLQDADWQTTRARIAAQYGYDRYGGNCHIVPNHALIILSLLYGADDFQRSLM
                     IVNSCGWDTDCNSGNVGCVLGIKNGLAGIEDGPDWRGPLADRLYLPTADGGRAITDAV
                     AETLHVVNAGRALAGAAPLAPKDGARFHWELPGSVQGFQVEQYPTSGATVWLENTARH
                     SRRGERSLALHFRHLAPGCPARVATPTFIPPDARAMPGYRLLASPTLYAGQMVSAGLS
                     AGDTNTRPVQCRLYISLYDADDRLVRRYGPDVVLGAGDWQELSWRVEDTGGAPIAGIG
                     VELNADGPAGGCVYLDYLTWDGPPDVELGHPVGTGEMWRRAWVDAVDQLEINRREPYR
                     LIQNRGTGLLIQGTRDWRDYRASADLTPHLVRAAGIGARVQGLRRYYALLLCADGVLR
                     LIKALHGTRELAAVPFLWEQGHTYRLTLQVEGAHLSAWVDDQLVLEVADDDQPLAGGG
                     VALLCQEGRLAAGPVRVQPALR"
     gene            complement(2877..>3269)
                     /locus_tag="VF276_03890"
     CDS             complement(2877..>3269)
                     /locus_tag="VF276_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013423748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX6606036.1"
                     /translation="QADTPMGRTIRSGVHLMQHFAEVPLLLIGFGRTRDGSGIYPALW
                     SAMLAARAEGIGATLTGMLQSYFADEVGEILGVPKDAGWYQHGVITMGYPTGKWGVAQ
                     RKPVHEVAARNSWQGDLGFTVPEPLWPQ"
BASE COUNT          509 a         1138 c         1096 g          526 t
ORIGIN      
        1 gaatactacg gcgacgccga gcgcatcctc cgcggccacc tgctgccgtc gcaactgcgc
       61 gatatctcgt ttatcgccgc gccgctcgat gccggcgcca cggacggcac gcgggatgtg
      121 gcgcggcggc atcgcggcgc cttcggcttt cctgccccct atgggcatga gccggtcggg
      181 atggaccatg tgagcttcaa catggatatc gtcggcgggg cggtggggtc gctgtgcgcc
      241 gcgtatcgcc acatcgcccg ccgcgatccg gacggccatc gcgtcaacct gctcttcgat
      301 cacgagaccg cagacctcaa ggtggagtcg ccctacaccc acccgcacct ccgagtgaca
      361 gtcaagcgcc cggcgcctct gttcgtacgt ctcccggcgt gggtccgccc cgaaacgatc
      421 cgcataccgg gactcgcagg gccgccccag attgtcgccg gctacctgac catccccgat
      481 ccacccgtca accaggcgtt gtccatcgag tttccgttgc cggaggcaga gatcgtgctg
      541 gcgcaccgta cccggcagat tcggacgcgg ctacgcgggg atcaggtgct tgccatggac
      601 aacttcggcg ccgatctgac gttcttcgat tcgatcgatt agccggaaca accccgcgcc
      661 cggcagggca ggggtactgg aggaggccgg aaaggcgcca tgaccttacc gaacgactac
      721 cttgagcgcg tgtatgccgg cgtgctgggc aagatgatcg gcgtctacct gggccgccca
      781 ttcgagggct ggacccacga gcgcatcatg gccgaactgg ggccgatcca gtattacgtg
      841 catgagcggc tgggcgtgcc gttgatcgtc accgacgacg acctgtcggg cacgttcact
      901 ttcctgcgcg cgctgcccga ccacggctac cggcgggacc tgacgcccgc cgagatcggg
      961 gagacctggc tcaactatat catcgagggg cgcacggtgc tctggtgggg cggactgggc
     1021 aactcgaccg aacacaccgc ctttctgcgg ctcaaagccg gcatcgaacc cccggccagc
     1081 ggctcgatgg ccctgaattc gcgcgtggtg gccgagcaga tcggcgcgca gatcttcatc
     1141 gacggctggg gcatggtggc gccgggcgac ccggaattgg ccgccgatct ggcgcgccgg
     1201 gcggcctgcg tgagccacga cggcgaggcg atctacggcg cgcaggtgct ggccgcgatg
     1261 gaggcgctgg cctttgtcga acccgatctt aaccggctgc tcgacacggc ggtggccctc
     1321 atcccgcggg actcgatcat ctatcgcctg atcgccgaca tccgcgagtg gcacctccag
     1381 gacgccgatt ggcagacaac gcgcgcccgc atcgcggccc agtacggcta tgaccgctac
     1441 ggcggcaact gccatatcgt gcccaaccac gccctgatca tcctcagcct gctgtacggc
     1501 gccgatgact tccagcggtc gctgatgatc gtgaatagct gcggctggga taccgactgc
     1561 aactcgggca acgtgggctg cgtgctgggc atcaagaacg gcctggccgg catcgaggac
     1621 ggccccgatt ggcgcggccc gctggccgac cggctctatc tgccgaccgc cgacgggggc
     1681 cgggcgatca ccgacgcggt ggccgagacg ctgcacgtag tcaacgccgg gcgggcgctg
     1741 gccggggcgg ccccgctggc gcccaaggac ggcgcgcgct tccattggga actgcccggt
     1801 tcggtccagg ggttccaggt ggagcaatac cccacctcgg gggccacggt atggctggag
     1861 aacaccgcca ggcacagccg ccgcggcgag cgcagcctgg cgctgcactt ccggcacctg
     1921 gcgcccggtt gccccgcgcg ggtggcgaca cctaccttca tcccgccgga cgcacgcgcc
     1981 atgccgggct accgcctgct cgcgtcaccc acgctgtacg cgggccagat ggtgtccgcc
     2041 ggcctgtctg ccggcgatac gaacacccgg cccgtccaat gccggctgta catcagcctc
     2101 tacgacgccg atgatcggct cgtccggcgc tacggtcccg atgtcgtgct cggtgccggg
     2161 gattggcaag agctatcctg gcgggtggaa gataccggcg gcgcgcccat cgccggcatc
     2221 ggcgtcgaac tcaacgcgga cggtccggcc ggcggctgcg tttatctgga ctacctcacc
     2281 tgggacggcc cgcccgatgt cgaactgggc catccggtcg gcacgggaga aatgtggcgg
     2341 cgggcctggg tcgatgcggt ggatcaactg gagatcaacc ggcgcgaacc ctaccggctg
     2401 atccagaacc ggggcaccgg gctgctgatc cagggcaccc gcgactggcg ggactaccgc
     2461 gccagcgcgg atctgacccc gcatctggtg cgggccgccg ggatcggcgc gcgggtccag
     2521 gggctgcggc gctactatgc cttactgctg tgcgcagacg gtgtgctgcg tctgatcaag
     2581 gcgctccacg gtacgcggga actggccgcg gtcccattcc tctgggagca ggggcacacg
     2641 taccggctaa cgctgcaagt tgagggcgcc cacctgtcgg cctgggtgga tgaccagctg
     2701 gtactggagg tggcagatga cgatcagccc ctggccggcg gcggcgtcgc cctactctgc
     2761 caagaggggc gcctggccgc ggggccggtg cgggtgcagc cggccctacg ctgaccttgt
     2821 ctgcccaatc tacgggcgca ggtgtaccgg ctcgtcagtt cttcgtctga agggaatcac
     2881 tgcggccaca agggctcggg cacggtgaac cccaggtcgc cctgccagct attgcgggcc
     2941 gccacctcgt gcaccggttt gcgctgcgcg actccccact tgccggtggg gtagcccatc
     3001 gtgatgaccc cgtgctgata ccagccggcg tccttcggca cccccaggat ctcacctacc
     3061 tcatccgcaa agtagctctg cagcatcccg gtcagggtcg cgccgatccc ttcggcgcgg
     3121 gcggccagca tcgcgctcca gagcgccggg tagatcccgc tgccatcacg cgtgcgaccg
     3181 aatccaatca gcagtaacgg gacctcggca aagtgctgca tcagatgtac ccccgagcgt
     3241 attgttcgcc ccatcggggt atccgcctg
//
LOCUS       DASUYC010000237         2465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_930291,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000237 DASUYC010000000
VERSION     DASUYC010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930291"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(309..1718)
                     /locus_tag="VF276_03895"
     CDS             complement(309..1718)
                     /locus_tag="VF276_03895"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein/redoxin"
                     /protein_id="HEX6606037.1"
                     /translation="MLILLLVAFVAGVLTILSPCILPVVPLVLGTASGGGRARPLGIV
                     AGLAGSFTLFSVVLASTLAALGLTTSTLRGGAVLTLAGFGLALLIPALGGRLEAALAP
                     LARVGARATGGATRRGDFAGGLVLGAGLGLIWAPCAGPIMGAVLALAATGGASPVTAA
                     VALAYAMGAGVPLLLIGYGSRALVARMRGAARRDGVQRALGGLMLLACLAILFNLDLR
                     AQDTVANALPADWSTRLYSVEQQPAVAQELRALQAPVPAIAAATVAPAPPAPMQPAIQ
                     KESPAMAEQLQPTAAPTATPARAPAAALPAPVEANLPAAVTLQNIGQAPEPVGITEWI
                     NSAPLTLQQLRGQVVLVHFWTFACINCIHVQPHVKAWYDRYHAAGFTVLGIHTPELSF
                     EREIANVRDAVKKDEVRFPVAFDPKYATWGAYNNGYWPAFYYVDKQGQIRYTHIGEGD
                     YAGQEQAIRQLLLEPASGS"
     gene            complement(1957..>2465)
                     /locus_tag="VF276_03900"
     CDS             complement(1957..>2465)
                     /locus_tag="VF276_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606038.1"
                     /translation="GGLMDMQARPGATWEETAQRLAPPDLSHLRLDDLVRHMGEGPLA
                     ALDESFRRSFFRSLMEEQPDGTVRPRLTRTRHMAILHAMWDQRLAPVLEQVACPILGI
                     LAEAPQPVDKADPFQAAKQRTLAQAAQHDNVHIVTLPDTIHDIPLHRPEELVRLIGDW
                     LATLPAPD"
BASE COUNT          380 a          819 c          899 g          367 t
ORIGIN      
        1 tgcgcatgct ggccgaggtc tggggcctcg attcctgacg tggccatccc ggcgcgctat
       61 ccccagcctg agccgatagg gcggcaggcc ggtttgaaac cggcccctac aacgcgggtg
      121 gggtagcgcg ggccggtttg aaaccggccc ctacaacgcc ggtagggcac gcgggccggt
      181 ttgaaaccgg cccctacaac gcgggtgggt tagcgcgagg cggattccaa ccggccccta
      241 cagcgtgggt gggccggtgg gctggtcgga aactggtttg tacaccgcaa agcgccggag
      301 gccggtggct aactgccgct tgccggctcc aggagtagct gccggatcgc ctgttcctgg
      361 ccggcgtaat cgccttcgcc gatgtgtgtg tagcggatct gcccttgctt gtccacgtag
      421 tagaaagccg gccagtagcc gttgttatag gcgccccagg tggcatactt ggggtcgaag
      481 gcgaccggga agcgcacttc atccttcttg acggcgtcgc gcacgttggc gatctcgcgc
      541 tcaaaggata gctccggcgt gtggatgccc agcacagtga agcccgcggc gtgatagcga
      601 tcgtaccagg ctttcacatg cggctgcacg tggatgcagt tgatacaggc gaaggtccag
      661 aagtggacca gcaccacctg cccgcgcagt tgctgcagcg tcagcggcgc cgagttgatc
      721 cactcggtga tcccgaccgg ctccggtgct tgcccgatgt tttgcagcgt gacggcggcg
      781 ggcaaattgg cttcgaccgg cgccgggagg gcggcggcgg gcgcacgcgc cggggtagcg
      841 gtgggggcgg cggtaggctg caattgctcg gccatggccg gggactcctt ctgtatggcg
      901 ggttgcatgg gcgccggggg cgcgggcgcg accgtggctg cggcgatggc cggcaccggg
      961 gcttgcaacg cgcgtagttc ctgggccacc gccggctgct gctcgacgct gtagagccgg
     1021 gtgctccaat cggcgggcag ggcgtttgcc acggtatcct gtgcgcgcag atcgaggttg
     1081 aacaggatcg ccaggcaggc cagcagcatc aggccgccta aggcgcgctg cacgccgtcc
     1141 cgccgggcgg ctccgcgcat gcgggccacc agcgcccggc tgccgtagcc gatgagcagc
     1201 aggggcacgc ccgcgcccat cgcgtaggcc agcgccacgg ctgccgtcac cggcgaggcg
     1261 cccccggtgg cggcgagtgc cagcacggcg cccatgatcg gcccggcgca gggcgcccag
     1321 atgaggccca ggcccgcgcc cagcaccagt ccgccggcga aatcgccccg ccgggtggcc
     1381 ccgccggtgg cacgagcgcc cacgcgggcc aggggcgcca gcgccgcctc cagccgcccg
     1441 ccgagtgccg ggatcagcag ggccagcccg aagcccgcca gcgtcagcac cgcccccccg
     1501 cgcaatgtgc tggtggtcag accgagcgcc gccagcgtgg aggccaacac gacgctgaac
     1561 agcgtgaagc tgcccgccag cccggcgacg atccccaggg gccgggcgcg cccgccgccg
     1621 ctcgccgtgc ccagcaccag gggcaccacc ggcaaaatgc acggcgagag gatcgtcagc
     1681 accccggcca cgaaggcaac cagcaagagg atcagcacgg cggtactcct ttgcgctgcg
     1741 gcggtccgcc cgcgtgggtg gggcggttat agacagaaca aacgcggggc cgtgaaagtt
     1801 ccccgcgcct attgccctgc cggtccgccg ctggccgcat aggagtccca tacaccaata
     1861 ttgtcattct gagcggagcg aagaatctcc gtctcgttcg ggataaggac gagactcttc
     1921 gctgcgctca gagtgacaag gctggtgtgg aaccgcttag tccggcgccg gcagcgtcgc
     1981 cagccaatcg ccgatgaggc ggaccagttc ttcgggccgg tgcagcggga tgtcgtggat
     2041 agtgtcgggc agggtgacaa tgtggacgtt atcgtgctgg gcggcctggg ccagcgtgcg
     2101 ctgcttggcc gcctggaacg ggtcggcttt gtccacaggc tggggcgcct cggcgagaat
     2161 gcccaggatg gggcaagcga cctgttccag caccggggcc aggcgctggt cccacatggc
     2221 gtgcaggatc gccatatgcc gggtgcgcgt cagccggggg cgcaccgtgc cgtcgggttg
     2281 ttcttccatg agcgagcgga agaagctacg ccgaaaggac tcgtccagcg cggccagcgg
     2341 gccttcgccc atgtgccgga ccaggtcgtc caggcgcaga tgtgacaggt caggcggggc
     2401 cagccgttgg gcggtctctt cccacgtggc gccgggccgg gcctgcatat ccatcagccc
     2461 gccgt
//
LOCUS       DASUYC010000238         2849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1432875, whole genome shotgun sequence.
ACCESSION   DASUYC010000238 DASUYC010000000
VERSION     DASUYC010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432875"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1432)
                     /locus_tag="VF276_03905"
     CDS             complement(<1..1432)
                     /locus_tag="VF276_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX6606039.1"
                     /translation="MDLPVHVDPDGAEPLHRQLYTQVRGAILAGRLPPGSRLPPTRHL
                     AAALGLSRNTVNEAYAALLAEGYLEARVGAGTYVAALLPDELLAPHPALTTPKAPAPA
                     PAPRRLSAWAGRIGAAVGPTFTPRRAVPLPYDFRPGLPAWDAFPATVWRRLVAGVWEE
                     IGPETLIYGDPAGYGPLRKEIAAYLGRTRAVRCRPEQIVVVSGSQQALDLLARLTLDP
                     GDAVAVEDPCYLGAQRVFSAAGARLVPVPVDSDGLVVEALPAQGARLLYVTPSHQYPT
                     GAILSLPRRLRLLEWAARTGTLVVEDDYDSQYRYTGRPLESLQGLDGAGTVVYTGSFS
                     TALFPALRIGYAVLPPDLVDLYRAAKATADREAASPLGHALALFMARGHFERHLRRMQ
                     ALYAGRRAALLAALARDLPEAVPGPAESGMHVMIYFSVPLDDTRLVAAAARAGVGLYS
                     ARPYYLHPPAQAAFLLGFASLPEATIA"
     gene            complement(1448..1753)
                     /locus_tag="VF276_03910"
     CDS             complement(1448..1753)
                     /locus_tag="VF276_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606040.1"
                     /translation="MEDVPMLCPHLHPLEAEILANGRQETFRGQAWSRHCREWVYFDC
                     WFDRAAIRARLPLAPCVQDHEHRGTHDGAEAGFVCAACEDGIMGYHPAYAAGKPVYR"
     gene            complement(1781..2599)
                     /locus_tag="VF276_03915"
     CDS             complement(1781..2599)
                     /locus_tag="VF276_03915"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEX6606041.1"
                     /translation="MIRLVALDLDGTLFGDDLVVSPANRAAIAAAQARGVHVTLATGR
                     MFRSARIHAAALDITTPLICYQGALVQDPVTEEILFHRPVAQALALEVIALLEEWGLY
                     PNVYLNDKLYTSELNPGTAYYSHVNGDLPIHPVGDLAAFLRQEGGDPTKLSTVLGNEE
                     RTTRTVALLREHFGERIYAVKSHPRFAETVNPHCDKGVALAMLADHLGIAQDQTLAIG
                     DGSNDLALLRWAGIGVAMGQAHAEVRAHADYVTGTLAEDGVAAALERFVLAPAG"
BASE COUNT          448 a          959 c         1053 g          389 t
ORIGIN      
        1 cggcgatggt ggcttcgggc agggacgcga agcccagcag gaacgcggcc tgggcgggcg
       61 ggtgcaggta gtagggccgg gcggaataga ggccgacgcc ggcgcgcgcc gccgcagcga
      121 ccaggcgggt atcgtccagg ggtaccgaaa agtagatcat cacgtgcatg ccgctttcgg
      181 cggggccagg cacggcctcc ggcagatcgc gggccagggc ggcgagcagc gcggcccggc
      241 gcccggcata cagcgcctgc atccgccgca ggtggcgctc gaaatgcccg cgggccatga
      301 acaacgccag cgcgtgcccc agcggcgacg cggcttcccg gtcggcggtg gccttggcgg
      361 cgcggtagag gtccaccagg tcgggcggca gcacggcgta gccgatgcgc agcgccggga
      421 aaagcgccgt cgagaacgag ccggtgtaga ccaccgtgcc cgcgccgtcc agcccctgca
      481 acgactccag cgggcgcccg gtgtagcggt actggctgtc gtagtcgtcc tcgaccacca
      541 gcgtgccggt gcgcgcggcc cattccagca ggcgcagccg ccggggcaga ctgaggatcg
      601 cgccggtggg gtactggtgc gacggggtga catagagcag gcgcgccccc tgggcgggta
      661 gcgcctcgac cacgaggccg tcgctgtcca ccggcaccgg caccagccgc gcgcccgccg
      721 cgctgaacac gcgttgcgcg cccaggtagc aggggtcctc cacggccacc gcatcgccgg
      781 ggtcgagggt caggcgcgcc agcaggtcca gcgcctgctg cgacccgctg acgacgacga
      841 tctgttccgg gcggcagcgc acggcgcggg tgcggcccag gtaggcggcg atctccttgc
      901 gcaaggggcc gtagcccgcc gggtcgccgt agatcagcgt ctccgggccg atctcctccc
      961 agaccccggc caccaggcgc cgccagacgg tagccgggaa ggcgtcccag gcgggcaggc
     1021 cgggccggaa atcgtagggc agcgggacgg cgcgccgggg cgtgaaggtc ggtccgaccg
     1081 ccgcgccgat gcgccccgcc caggccgaca accggcgcgg ggccggcgca ggcgcggggg
     1141 ctttgggggt ggtgagcgcg ggatggggcg ccaataactc gtcgggcaag agcgcggcga
     1201 cataagtgcc agcgccgacg cgggcctcca gatagccctc ggccagcaag gcagcatatg
     1261 cctcgttgac cgtgttgcgc gagaggccga gcgccgctgc caggtgccgg gtcgggggca
     1321 agcggctgcc gggcggcagg cgcccggcga ggatagcgcc acggacctgg gtgtatagct
     1381 ggcggtgcag cggttccgcg ccgtccggat cgacatgtac cgggagatcc acgctgctgc
     1441 cctcgcgcta gcgatagacg ggcttacccg ccgcatacgc cgggtggtag cccatgatgc
     1501 cgtcttcgca tgcggcgcag acaaagcccg cctcggcgcc gtcatgggtg ccgcggtgct
     1561 cgtggtcctg cacgcagggc gccagcggca accgggcacg gatggcggcc cggtcgaacc
     1621 agcagtcgaa atagacccac tcgcggcagt ggcggctcca ggcctggccg cggaacgtct
     1681 cctggcggcc attggcgagg atctcggctt cgagcgggtg caggtgcggg cagagcatgg
     1741 gcacgtcttc catcggggtg ctaacttgcc gggcgcggcg tcagccggcg ggggccagga
     1801 cgaagcgctc cagcgccgcc gccacgccat cctcggccag ggtgccggtc acgtaatcgg
     1861 catgggcgcg cacctcggca tgggcctgac ccatcgccac gccgatgccc gcccagcgca
     1921 gcagcgccag atcgttgctg ccgtcgccga tcgccagcgt ctggtcctgg gcgatgccca
     1981 ggtggtcggc cagcatcgcc agggccacgc ccttgtcgca gtgggggttc acggtttcgg
     2041 cgaagcgcgg gtgcgacttc acggcgtaaa tccgttcgcc gaagtgttcg cgcagcaggg
     2101 cgacggtgcg cgtggtgcgc tcctcgttgc ccaacaccgt cgagagcttg gtggggtcgc
     2161 cgccctcctg gcgcaggaat gcggccaggt cgcccaccgg gtggatgggc agatcgccgt
     2221 tcacgtgcga ataataggcc gtgccgggat tgagttcgga ggtgtagagc ttgtcgttga
     2281 ggtagacgtt ggggtagagg ccccactcct ccagcaaggc gatgacctcc agcgccagcg
     2341 cctgggccac ggggcgatga aacaggatct cttcagtcac ggggtcctgg accagcgcgc
     2401 cctggtagca gatcagcggc gtggtgatat ccagcgccgc cgcatggatc cgcgccgacc
     2461 ggaacatgcg ccccgtcgcc agggtcacat gcaccccgcg cgcctgggcc gccgcaatcg
     2521 ccgcccggtt cgccggacta acgaccaaat cgtcgccaaa caacgtccca tcgagatcta
     2581 acgcaaccaa tcgaatcatg tctgtttcct tagcggtcag tggtcagtgt cagtggtcag
     2641 ttgagacggg tgttgtgtgc taaatacaaa ctgatcactg atcactgatc actgaccact
     2701 gaccactgac cactgaccac tcgtccgcac tcgtctgcac ccagaggatg cggtcgcggt
     2761 gttcgaggtc gtgcagcacg cggagttccg cgccgggaaa ggctgcggcg gcggcgggca
     2821 agagcagagg agcctggtcc cagccgatt
//
LOCUS       DASUYC010000239         3844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_505341,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000239 DASUYC010000000
VERSION     DASUYC010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505341"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VF276_03920"
     CDS             <1..559
                     /locus_tag="VF276_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6606042.1"
                     /translation="HGSLESAITGLHVGVFDYLLKTADPQEVLTRVAAAIRRGQEERR
                     KQELLATLQSVVGELGGRPTPAPRPAEPAEGWIAIGDLQISTWKQVARVGDQTLNLTP
                     TEFRILVCLAQQAGQVMTYQQIVRCAQGYDTQALEAAELIKPHIYHLRQKIEQNPSDP
                     RYILTVRGTGYLLAAGPDQVPDEPD"
     gene            complement(569..2473)
                     /locus_tag="VF276_03925"
     CDS             complement(569..2473)
                     /locus_tag="VF276_03925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX6606043.1"
                     /translation="MQQRLWSFVKENPIPPFALLGLIAGTVLTFWVHPQHITELITGG
                     SFAFGWQDLDIADWVWLITLVVGGAPLVWSTLKGMLRGRFASDVVAMLAILTAVVMGE
                     YFAGVIVVLMQSGGEALERYGLRRASSSLEHLLARAPRTARRKQGDRLEEIDVAQVAI
                     GDHLVVRPGELIPVDGTLLSVSADVDESAVTGEPLAAGKQRDDRLLSGSVNAGGPFEM
                     QATALSSQSQYARIVALVRQAQDEKPPIGRLADQYAVWFTPLTLLMCGLGWFITGDPR
                     TILAVLVVATPCPLILATPIAIISGINRAADRGIIVKGGAAIEQVGRAKAVVFDKTGT
                     LTLGTPVLDRVTLFNGHSTGGLLRKAASVEQLSSHLLGRTMVQAAQADGGSLPLPVNF
                     QEVPGRGVEGHVEGEHILVGSPRFLAERLGAEHVESVTALREHAGAGDMLAAFVAVDL
                     APAGVVIFNDKLRPGVPDLVRRLRLLGVQRTVMLTGDRLANARSIAQEAGVDQVHADL
                     LPADKVAVLQKLKDQYEPIVMVGDGINDAPALATATVGIAMGAHGTGVSAEAADIVLL
                     VDDVTRVGDAVEVGQRTLRVARQSIYAGLGLSFGLMVAAAFGLIPPAIGALAQEVVDV
                     AVIVNALRAR"
     gene            3048..>3844
                     /locus_tag="VF276_03930"
     CDS             3048..>3844
                     /locus_tag="VF276_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEX6606044.1"
                     /translation="MTDMLLDTWKLVEAGFPRSGTPADQLGFLLRYAVRAPSGHNTQP
                     WLFHLTGDAVELHGDRRRAMPAVDPGDRELIMSCGAALLHLRIALHHFGYTGDVTLFP
                     DATRPDYLARVGLGRPGPEPPEDEYLFRTIPHRRTHRLKFDEHPVPVPLQTALQAAAA
                     AEGAGLHLIHDRDQQAAVADLVAAGDALLADDAQFREELAAWVRSNHDLTGDGLPGYA
                     FGAGDVASHFLPFLIRKFDLLQGQAAKDQTLAAEAPLLAVLDTPADTP"
BASE COUNT          654 a         1373 c         1248 g          569 t
ORIGIN      
        1 ccacggctcg ttggagagcg ccatcaccgg gctgcacgtc ggtgtgttcg actacctgct
       61 caaaaccgcc gacccgcaag aggtgctgac tcgtgtggcc gccgctatcc gccgcgggca
      121 ggaggaacgc cgcaagcaag agttgctggc aacgctgcaa agtgtggtcg gcgagttggg
      181 cgggcgcccc acgccggccc cgcggcccgc cgagccggcg gaaggctgga tcgccatcgg
      241 cgacctgcag atctccacct ggaagcaggt ggcccgcgtc ggcgaccaga cgctgaacct
      301 gacccccacc gagtttcgca tcctggtttg cctggcccag caggccgggc aggtgatgac
      361 ctaccagcaa atcgtgcgtt gcgcgcaggg ctacgatacc caggccctgg aagccgccga
      421 actgatcaag ccgcacattt accacctgcg gcaaaagatc gagcagaacc catccgaccc
      481 gcgttatatt ctcaccgtgc gcggcaccgg ctatctgctg gccgccggcc ccgatcaggt
      541 accggacgag ccggactgag cgggcgcgct aacgcgcccg caaagcattg acgatgaccg
      601 ccacgtcgac gacttcctgg gcgagcgcgc cgatagccgg cgggatcagg ccgaaggcgg
      661 cggccaccat caggccgaag ctcaggccca gcccggcata gatactttgc cgcgccacgc
      721 gcaaggtccg ctgtcccacc tccaccgcat cgccgacgcg ggtcacgtcg tccaccagca
      781 gcacgatgtc ggcggcttcc gccgacacgc ccgtgccgtg cgcgcccatg gcgatgccga
      841 ccgtggccgt cgccagcgcg ggcgcgtcgt tgatcccgtc gcccaccatg acgatgggct
      901 catactggtc cttcagcttt tgcaacacgg ccactttatc cgcgggtagc aggtcggcgt
      961 ggacctgatc gactcccgcc tcctgcgcga tgctgcgcgc gttcgccagc cggtcgccgg
     1021 tcagcatcac agtgcgctgc acgcccagga ggcgcagacg gcgcacgaga tcgggcacgc
     1081 cggggcgtag cttgtcgttg aagatgacca cccccgccgg cgccaggtcc accgcgacga
     1141 aggcggcgag catatcgccc gccccggcgt gctcacgtag cgcggtgacg ctctcgacat
     1201 gctccgcccc caggcgttcg gccaggaagc gtggcgaacc gaccaggata tgctcgccct
     1261 cgacatgccc ttcgacgccg cgccccggca cttcctggaa attgaccggc aagggcagcg
     1321 acccgccgtc ggcctgggcc gcctgcacca tcgtccggcc cagcaggtgg gaagacagtt
     1381 gctcgacgct ggctgccttg cgcagcaggc cgccggtgct gtgcccgttg aacagtgtca
     1441 cccggtcgag cacgggcgtg ccgagggtca gggtgcccgt cttgtcgaac acgaccgctt
     1501 tggcgcggcc cacctgctcg atggcggcgc cgcccttgac gatgatgcca cggtcggcgg
     1561 cccggttgat cccgctgatg atggcgatgg gtgtggcgag gatcaggggg cacggcgtcg
     1621 ccaccaccag cacggccagg atcgtgcgcg ggtcgccggt aatgaaccag cccaggccgc
     1681 acatgagcag cgtgagcggg gtaaaccaga cagcatattg gtcggccagc cggccgatgg
     1741 gcggtttctc gtcctgtgcc tggcgcacca gcgcgacgat ccgggcatac tggctctggc
     1801 tgctcagcgc cgtcgcctgc atctcgaacg ggcctcccgc gttcacactc ccgctcaaca
     1861 gccggtcgtc gcgctgtttg ccggcagcca gcggctcccc ggtgacggcg gactcgtcca
     1921 cgtcggcgct caccgacagc agcgtgccgt ccacggggat taattcgccg ggccgcacca
     1981 ccaggtgatc gccgatcgcc acctgggcca cgtcgatctc ctccagccga tccccctgtt
     2041 tgcggcgggc ggtgcgcggg gcgcgcgcca gcaaatgctc cagcgacgac gaggcccggc
     2101 gcaagccgta gcgctccagc gcttcgccgc ccgactgcat gaggaccacg atgaccccgg
     2161 cgaagtattc gcccatgacc acggcggtga gaatcgccag catcgccacc acatcgctgg
     2221 cgaaccggcc ccgcagcatc cccttcagcg tggaccagac cagcggcgcc ccgccgacga
     2281 ccagggtgat gagccagacc cagtcggcga tgtccagatc ctgccagccg aaggcaaagg
     2341 acccgccggt gatcagctcg gtaatgtgtt gcggatgtac ccagaaggtc agcacggtgc
     2401 cggcgatgag gccgagcagc gcaaacggag gtatgggatt ctctttaaca aaagaccata
     2461 atcgctgttg catgatttgc cgggttgcgg gggagtgggg ggagagacga gcttcgagcg
     2521 atgcggcgct gccatccaca ggccgcaccg ctcgaagaag cggatccttg ctctcactgt
     2581 ccgggtaggc aggccagaaa agccggtatc cgtcgttcgc actttcacta tacccgcaac
     2641 caacctcact ttctgtgaag aatcgaccac ggcgggtggt gccttgctca ccggcgcccg
     2701 ccagggcggg gcaccgcgcg gggcgatcgc cccgatcgcc gtcgccgcta agccgtccgt
     2761 ccccggaacg gctgctgaaa acgacaatgc gccggctccg gaggcgaacg tggaactgga
     2821 cgggccacgg ggtagacgca cctgccttaa gtataagcgg cgagccgggc gcctgcggta
     2881 aagaaagcac cacagccgat gaagtctcca cgacggcgca caaggggcgc cggcccccgc
     2941 ctgctaaccg cgggtggtga actcttcacc ggcatcccgg cgtctgggcg ctattataag
     3001 gatgaaggat gacggtaagg tgccgttcct aggaggagat tcccgtcatg accgacatgc
     3061 tgctcgacac ctggaaatta gtggaagcag ggttcccccg cagcgggacg ccggcggacc
     3121 agctcggctt tttgctgcgc tacgccgtgc gtgccccttc cgggcacaac actcagccct
     3181 ggctgttcca cctcacgggc gacgccgtgg aactgcatgg cgaccggcgc cgggccatgc
     3241 ccgccgtgga tccgggcgac cgggaactga tcatgagctg cggcgcagcc ctgttgcacc
     3301 tgcgtatcgc gctgcaccac ttcggctaca cgggcgacgt gaccctgttc cccgacgcga
     3361 cccggcccga ctacctggcg cgcgtggggc tgggccggcc cggcccggaa ccgccggaag
     3421 acgagtacct gttccgcacg atcccccatc gccgcaccca tcgcctcaag ttcgatgaac
     3481 acccggtgcc cgtcccgttg caaaccgcgc tccaggccgc cgcggcggcg gaaggcgccg
     3541 ggctacacct gattcacgac cgggatcagc aagccgccgt ggccgatctg gtcgcggcgg
     3601 gcgacgcgct gctggccgat gacgcgcaat tccgcgagga actggccgcc tgggtgcgct
     3661 cgaaccacga cctcaccggc gacggcctgc ccggttacgc cttcggcgcc ggcgacgtgg
     3721 cctcgcactt cctgcccttc ctgatccgca aattcgacct gctacagggg caagccgcga
     3781 aagatcagac gctcgccgcc gaggcgccct tgctggccgt gctcgacacc cccgccgata
     3841 cccc
//
LOCUS       DASUYC010000240         3153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_785133,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000240 DASUYC010000000
VERSION     DASUYC010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785133"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2878)
                     /locus_tag="VF276_03935"
     CDS             complement(<1..2878)
                     /locus_tag="VF276_03935"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6606045.1"
                     /translation="MSNLVRAGRLPRTARAAAPPRPPSELRRTTGLLWRYLAGQQRVF
                     IQGGIMLLVESLTAVLPPLLIAYLIDKYITPRVDQLTGKAAVAPLSPLQAVGLPSVFG
                     PDIDTVALVVLGLILLTALNSMADSLAEIYLARGGRTLGLNLRVALYSHLQRLSLAFY
                     NQRRTGDLLTRVTGDVTALEDFVTASLSDIAGSILLIIWSVIAMVLYGWQVAIVAAVI
                     IPIMALISNYFSQRIKAASKKQRAREGELAAAAQQMLTSIRVIQTYGRGGNEAQQFAK
                     QSRQTMEAALEAAGLQARFSWVVSVLEAAATAAVICLGVWLILPPQAAIKVGTLLAFI
                     TLMQNMFKPTKKIIKEWNTVGKIYASVERIGELLDRKPAVQDAPNAVAAPPLRGDIEF
                     RHVGFAYQPEPEDMKPGAPPPPLRMTLKDVNFHVPPGGVVALVGPTGAGKSTILQLLP
                     RLYDPHTGQIMIDGHDSREFTMDSLRGQMSMVLQESILFTGSIADNIAYGREGATREE
                     IIAAAIQANAHEFIEKLPEGYDTLLGERAGNLSGGQRQRLAIARAFIRNTPILILDEP
                     TTGLDAESTDLVLLALRTLMKGKTTLIISHDLNLIRHVDQILVLKDGQIIQTGTHKEL
                     LKAGGLYADLYNMQFGRAAEEAGTPLPKPAAEPEPDAEPEAVSPLAFQTMLMQALPLP
                     VTSQAFETLATQRPRLDTNGAGTEHPAGGRAAPAAPRAPAAPAAASKEAPPPETGRAP
                     IFQTTMMRVLPRRGPQDGTIPKEARMSDQAPLATPASPPAQKTDGHAYIPAVRASKEV
                     PAAPANPDGGVAVAAADAPPAALMQGAPPAVVASDAPSAVAAQAAPPAVVSAGPPAAT
                     PPAATPEAPRRRDPQSIAEVGGAPVDPLQSPLLQQDLPGLVEAFDGEAMRERLQAALF
                     GKGNPNYTIERATPGQAIYQPGESCVLRYSLEVRDSRSGQILKPL"
     gene            complement(2875..>3153)
                     /locus_tag="VF276_03940"
     CDS             complement(2875..>3153)
                     /locus_tag="VF276_03940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018856661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6606046.1"
                     /translation="AVSGVPELVQHETNGLLVPPHDAPALAAALAALLDDPARRARLG
                     QAAQRTVADHFDLRAAAGQLAALFGRRPAPGPVVAADIVTDQGEKVLL"
BASE COUNT          437 a         1048 c         1167 g          501 t
ORIGIN      
        1 ccagcggctt gaggatctgg ccgctgcggc tgtcgcgcac ctccaggctg tagcgcagga
       61 cgcaggactc gcccggctgg tagatggcct ggcccggcgt ggcccgctcg atggtgtagt
      121 tggggttgcc cttgccgaag agcgctgcct gcaagcgttc gcgcatcgcc tcgccgtcga
      181 acgcctcgac cagaccgggc aaatcctgct ggagcagggg actctgcagc gggtccactg
      241 gcgccccgcc gacctcggcg atgctctgcg ggtcgcggcg gcgcggcgct tccggcgtgg
      301 cggcgggcgg cgtcgcggcc ggtggcccgg cggacacgac ggcgggcggt gcggcttgcg
      361 cggccaccgc cgagggcgca tccgaggcca caacagcggg cggcgcgccc tgcatcagcg
      421 cagccggcgg cgcgtccgcc gccgcgacgg cgactccgcc gtccgggttg gccggggccg
      481 ccgggacctc tttagaggcc ctaacggcgg ggatgtaggc gtggccgtcc gtcttttgcg
      541 cgggcgggga cgcgggagtc gctagcggtg cctggtcgct catcctggcc tccttcggga
      601 tagtgccatc ctgcgggcca cgccgcggga ggacgcgcat catcgtggtc tggaagatcg
      661 gtgcccgccc ggtttccggc ggcggggcct ctttagacgc ggcagcgggc gcagccggcg
      721 cgcggggcgc ggcgggcgcg gcgcggccac cggcggggtg ttccgtgccc gcgccgttgg
      781 tatcgaggcg cggcctctgg gtcgccagcg tctcgaacgc ctgcgaggtc acgggcagcg
      841 gcagcgcctg catgagcatg gtctgaaacg ccagcgggct gaccgcttcc ggctcggcat
      901 cgggttccgg ctccgcggcg ggtttgggca gcggcgtgcc cgcttcctca gcggcccgac
      961 caaactgcat attgtagagg tccgcataga ggccgccggc tttgagcaac tctttatgcg
     1021 tgcccgtctg gatgatctgc ccatccttca gcaccaggat ctggtccacg tggcggatca
     1081 ggttgaggtc gtgcgagatg atgagcgtcg tcttgccctt catcagcgtg cgcagagcca
     1141 gcagcaccag gtcggtggac tcggcgtcga ggccggtggt cggctcatcg aggatgagga
     1201 tgggcgtgtt gcggatgaag gcgcgggcga tggcgaggcg ctggcgctgg ccgccggaga
     1261 ggttgcccgc gcgctcgccc agcagtgtgt cgtagccctc cggcagcttc tcgatgaact
     1321 cgtgggcgtt ggcctggatc gcggcggcga tgatctcctc gcgcgtggcg ccctcgcgcc
     1381 cgtaggcgat attgtcggcg atgctgccgg tgaacaggat gctttcttgc agcaccatgc
     1441 tcatctgccc gcgcagcgag tccatggtga actcgcggct gtcgtggccg tcgatcatga
     1501 tctgcccggt gtgcgggtca tagaggcggg gcagcagttg caagatggtg ctcttgcctg
     1561 cgcccgtcgg ccccaccagg gccaccaccc caccgggcgg cacgtggaag ttaacatcct
     1621 tgagcgtcat gcgcagcggg ggcggcggcg cgcccggctt catatcctcc ggctcgggct
     1681 gataggcgaa gcctacgtgg cggaactcga tgtcgccgcg cagcgggggc gctgcgaccg
     1741 cgttgggcgc gtcctgcacg gcgggcttgc ggtccagcaa ctcgccgatg cgctccacgc
     1801 tggcgtagat tttgccgacc gtgttccact ccttgatgat cttcttggtc ggcttgaaca
     1861 tattctgcat cagggtgata aaggcgagga gcgtcccgac tttgatcgcc gcttgcgggg
     1921 gcaaaatcaa ccagacgccg aggcagatca ccgccgccgt ggccgccgcc tccagcacgc
     1981 tgaccaccca actgaagcgc gcctgcaagc ccgccgcctc cagcgcggcc tccatcgtct
     2041 gccggctctg cttggcgaat tgctgggctt cgttgccgcc gcgcccgtag gtctggatca
     2101 cgcggatcga ggtgagcatc tgctgggcgg cggcggccag ttcgccctcg cgcgcccgct
     2161 gcttcttcga ggcggccttg atgcgctgcg agaaatagtt cgagatcagg gccatgatcg
     2221 ggatgatcac cgcggcgacg atcgccacct gccagccgta gaggaccatg gcgatgacgc
     2281 tccagatgat gagcaggata ctcccggcga tgtccgacag cgaagcggtg acgaagtcct
     2341 ccagcgcggt cacatcgccg gtcacgcggg tgagcaggtc gcccgtgcgc cgctggttgt
     2401 agaaggccag cgagaggcgt tgcaagtgcg aatagagggc cacgcgcagg ttcaagccga
     2461 gcgtgcgccc gccccgcgcc aggtagatct cggccaggga gtcggccatg ctgttgaggg
     2521 cggtgagcag gatcaggccg agcaccacca gggccacggt gtcgatgtcc ggcccgaaga
     2581 cgctcggcag ccccacggcc tgcagcgggc tgaggggcgc cacggcggct ttgccggtca
     2641 actggtccac gcgcggggtg atgtacttgt cgatcaggta ggcgataagc agcggcggca
     2701 gcaccgcggt gagcgattcg acaagcagca tgatcccgcc ctggatgaac acgcgctgct
     2761 ggccggccag gtagcgccag agcagcccgg tggtccgccg cagttcgctc ggcgggcggg
     2821 ggggcgccgc ggcgcgggca gtgcgcggca ggcgcccggc acgaacgaga ttgctcacag
     2881 taacaccttc tccccttgat cggtgacgat atcggcggcc acgaccggcc ccggtgcggg
     2941 ccgccgcccg aagagcgcgg ccaattgccc ggctgccgcg cgcagatcga aatggtcggc
     3001 cacggtgcgc tgggcggcct gccccaggcg ggcgcggcgc gccggatcgt cgagcagcgc
     3061 ggccagcgcg gcggccagcg ccggcgcgtc gtgcggcggc accagcagcc cgttcgtctc
     3121 gtgctgcacc agttcgggca cgcccgacac ggc
//
LOCUS       DASUYC010000241         3462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1793048, whole genome shotgun sequence.
ACCESSION   DASUYC010000241 DASUYC010000000
VERSION     DASUYC010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1793048"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3462)
                     /locus_tag="VF276_03945"
     CDS             complement(<1..>3462)
                     /locus_tag="VF276_03945"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX6606047.1"
                     /translation="VILLLGMMHVRAALGTPAMAVLLPLVAIYAGALLMRVRAVLPAG
                     ARRRSAAPRAGDTAAGLQLPLDAAFILGVTVAGVHGGSLSVPFMVILAGTRFFAWNPG
                     RPTWRLLLPFALPLLVLAGRAYDTLGDSVVIAALVRETMLTECLLFMVYLGTRQMNQG
                     FTAVAELATAVAERDQRLDEAEHRAEKDARRLAEQVLYLTVLQNGIAAINSAMALDDL
                     LQMIVTNAVRVLKAEQSSIGLIDEATGELVIQAATGVKASTLRRQRFRPGFGVAGLVM
                     QTGEPLLVGDVLQDPRYVHPYAEDGDDATKRPAGAVTRTILCVPLMVEHRVIGVLSAT
                     HSQPNALSQDDQRWLISFAEQAALAVYRTRLLEERTQQGEELRRRGEILSSLMSIRQA
                     MVSSLNLQQVLDTTISRIGELIRFDYGLIYLHHRRTGNFELAVTNSDPATVPAALTMA
                     PDTLAMRLWDQALPTGSAMGAGDELCVLSLPLVNRGKTLGCILLAREAASPFAELERD
                     AAYQMASAAAIAVDNARLFSRVSKQEQQATALYRLMIKVSDAADRRQLAQVVCEELQH
                     IAGARAAALLVHDTDQARFIGWAATGDWQDRHDVTSVVLAAHGDPFVSNILLALQQRT
                     SPDLLVFPEVPSQGDDIFDGPGCVTIPLTMARRIYGLLILRPGTDPAIEPESRELVNL
                     AVSHCTVALERAELFEQTLAAARQSGILHKIGTEVQASLDPVTVVQTMASGALLALPI
                     DSCEVYIFDAERKNLLRTGVGVSRTAEEVGWRPGPDRIPVEGNASVQEVLHSRGLVTD
                     DVRVGTRSLSDTFGVSVVLGRLMGSAEPLGIVRLRTVLPAEEFIRRHATFCQTLWMHS
                     GGALERSHLYTTTANQARMLRQRAQQLTDILDLGHLSAADVPLDTLLPQIAAGITRSL
                     GFAAVEIREIDAESAADALARSEPAPGMVPLSLLERMLQAGHRPRADFNGVYIEEARL
                     ANILHRPPQPGIVSVGRPHILMPLDSTGGGALGYLMATPALHTEITDAQSEHELLEVL
                     SIFAQRTALIVENHRVYSQLLTSKRKIESVVLSISDGVIVTDEELNIMITNSLADQLL
                     GVPAAESRDLPLHRFIDNAALLAQVEECITTGVSTTADVDFFLGREERTYQAVVHPIA
                     ALEMGMLG"
BASE COUNT          542 a         1117 c         1266 g          537 t
ORIGIN      
        1 gcccagcatg cccatctcca gggcggcgat ggggtggacc acggcctggt aagtccgctc
       61 ctcgcggccc aggaagaagt cgacatcggc ggtggtcgag acgccggtgg tgatgcactc
      121 ttccacctgg gccagcagcg ccgcgttgtc gatgaagcgg tgcagcggca ggtcgcggct
      181 ctccgccgca gggacgccca gcaactggtc ggccaggctg ttggtgatca tgatgttcag
      241 ttcttcgtcg gtcacgatca cgccgtcgct gatcgagagc acgaccgact cgatcttgcg
      301 cttgctggtc agcagttgcg agtagacgcg gtggttctcc acgatcaggg cggtgcgctg
      361 ggcgaagatg ctcagcactt cgagcagttc atgctcggac tgggcgtcgg tgatttcagt
      421 atgcaaggcg ggggtcgcca tcagatagcc gagggcgccg ccgccggtcg agtccagcgg
      481 catgaggata tgcgggcggc ccacgctgac gatgccgggc tgcgggggcc ggtgcaggat
      541 attcgccagg cgcgcttcct cgatgtagac gccgttgaaa tcggcgcgcg ggcggtgccc
      601 cgcctgcaac atgcgttcca gcaggctgag gggcaccata ccgggggcgg gctcgctgcg
      661 ggccagggcg tccgccgcgg attcggcgtc gatctcgcgg atttccacgg cggcaaagcc
      721 gaggctgcgg gtgatcccgg cggcgatctg gggcagcagc gtatccagcg gcacatcggc
      781 ggcggacaga tggcccagat cgaggatgtc ggtcagttgc tgggcgcgct ggcgcagcat
      841 gcgcgcctgg ttggccgtcg tggtgtagag atggctgcgt tcgagcgcgc cgccggagtg
      901 catccagagc gtctggcaga aggtggcgtg gcggcggatg aactcctcgg cgggcaggac
      961 cgtgcgcagg cgcacgatgc ccagcggctc ggcgctgccc atgagccggc ccagcaccac
     1021 gctgacgccg aaggtatcgc tcagcgagcg cgtgcccacg cgcacatcgt ccgtgaccag
     1081 cccgcgcgag tgcagcactt cctggaccga ggcgttgccc tcgaccggga tgcggtcggg
     1141 gccgggccgc cagcccactt cttccgccgt gcggctcacg ccgaccccgg tgcggagcag
     1201 attcttgcgc tcggcgtcga agatgtagac ctcgcaggaa tcgatgggca gggccagcag
     1261 cgcgccagag gccatcgtct gcaccaccgt gaccgggtcg agcgacgcct ggacttcggt
     1321 gccgatcttg tgcaggatgc ccgactggcg ggcggcggcc agggtttgct cgaacagttc
     1381 ggcgcgctcc agggccaccg tgcagtggga cacggccagg ttgaccaatt cgcggctctc
     1441 gggttcgatg gccgggtcgg tgccggggcg caagatgagc aggccgtaga tgcgccgggc
     1501 catggtcagc gggatggtga cgcagccggg gccgtcgaag atatcgtcgc cctggctggg
     1561 cacctcgggg aacaccagca gatcgggcga ggtgcgctgt tgcaaggcca gcaggatgtt
     1621 gctcacgaag ggatcgccgt gggcagccag caccacgctg gtgacatcgt ggcgatcttg
     1681 ccaatcgccg gtggcggccc agccgatgaa gcgggcctgg tcggtgtcgt ggaccagcag
     1741 cgccgcggcg cgcgcgccgg cgatgtgttg caattcctcg cagaccacct gggccagttg
     1801 ccgccggtcg gcagcgtcgc tgaccttgat catgaggcgg tagagcgcag tggcctgctg
     1861 ctcttgcttc gatacccggc tgaacagccg ggcattgtct acggcaatgg cggccgcgct
     1921 ggccatctgg taggcggcgt cgcgctccag ttcggcgaag gggcttgccg cttcgcgggc
     1981 gagcaagatg caaccaagcg tcttgccgcg gttcaccagg ggcaggctga gcacgcagag
     2041 ttcgtcgccg gcgcccatcg ccgaaccggt gggcagtgcc tggtcccaca gccgcatggc
     2101 cagcgtatcg ggggccatcg tcagcgcggc gggcaccgtc gcgggatcgc tgttggtcac
     2161 ggccagttca aagttccccg tgcgccggtg atgcagatag atcaggccgt agtcgaagcg
     2221 gatcaactcg ccgatgcgcg agatggtcgt gtcgagcacc tgttgcagat tcaacgaact
     2281 gaccatcgcc tggcggatcg acatcaggct ggagaggatc tcaccgcggc ggcgcagttc
     2341 ctcgccctgt tgcgtgcgtt cttcgagcag ccgggtgcgg tagacggcca gggccgcctg
     2401 ctcagcgaaa ctgatcagcc agcgctggtc gtcctggctg agcgcgttgg gctgcgagtg
     2461 ggtcgcgctc agcacgccga tgacgcggtg ctcgaccatg agcggcacgc agaggatcgt
     2521 ccgcgtgacc gccccggcgg ggcgcttggt cgcgtcgtcg ccgtcctcgg cgtaggggtg
     2581 gacgtagcgc gggtcctgca acacgtcgcc gaccagcaag ggctcgcccg tctgcatcac
     2641 caggccggcc acgccgaagc cggggcggaa acgctggcgg cgcagcgtgc tcgccttgac
     2701 gccggtggct gcctggatga ccagttcgcc ggtcgcttcg tcgatcaggc cgatggagct
     2761 ttgctcagcc ttcagcacac ggacggcatt ggtgacgatc atttgcagca gatcgtcgag
     2821 ggccatagcc gagttaatcg cggcgatccc gttctgtaac acggtgagat agagcacctg
     2881 ctcggccagc cggcgggcgt ccttctcggc gcggtgctca gcctcgtcga ggcgctgatc
     2941 gcgctcggcc acggcggttg ccagctcggc gacggcggtg aagccctggt tcatctggcg
     3001 ggtgcccaga tagaccatga acagtagaca ctcggtcagc atcgtctcgc ggaccagcgc
     3061 cgcgatcacc accgaatcgc ccagggtatc gtaggcgcgc ccggccagca ccaggagcgg
     3121 cagcgcgaac ggcaacagca accgccaggt gggccggccg gggttccagg cgaagaaccg
     3181 cgtcccggcc aggatgacca tgaacggcac cgacaggctg ccgccgtgga cgccggccac
     3241 cgtgacgccg aggatgaagg cggcgtcgag cgggagctgc agcccggctg ccgtgtcgcc
     3301 cgcgcgcgga gcggcggatc gccggcgcgc cccggcgggc agcacggccc gcacccgcat
     3361 cagcagcgcc ccggcataga tcgccaccag cggcagcagg acggccatgg cgggcgttcc
     3421 cagcgccgcg cgcacatgca tcatgcccag gagcaggatc ac
//
LOCUS       DASUYC010000242         2288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_365484,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000242 DASUYC010000000
VERSION     DASUYC010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365484"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VF276_03950"
     CDS             <1..429
                     /locus_tag="VF276_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6606048.1"
                     /translation="FDHVAPPAAPIPPADALAGNIDGLRGFRVPCLLISPWSPRATVA
                     HNVYDHTSILKLIEWRWDLPPLTVRDASANNLATALDFSQSNLAAPTPTVPPGPFGGA
                     CPAGESGPDDEWQMLRDLAQKYGWPIYGPRPVLSGKEHTR"
     gene            426..>2288
                     /locus_tag="VF276_03955"
     CDS             426..>2288
                     /locus_tag="VF276_03955"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEX6606049.1"
                     /translation="MMQSPTLAPAARRIPRPLLFALLAGLAFAMAGLVGTGSAALSPA
                     PVPAALAAAPLGCPPGFVITASPNISNSLNTFYAVAARAAGDVWAVGNYDNGGILQTL
                     TAHYDGAAWTAVPSPNQGSTNNTLQGVAALAPDDAWAVGGYGEAITQSQTLIEHWNGS
                     AWSLATPPAVSGANGLLAVSAVAPNDVWAVGSAANIIGAPQTFIAHWNGSAWSHIASP
                     NGTALGLNVLTSVSARAADDVWAAGFYTDLSGVSYTLIEHWNGTAWSVIDSPERGTGD
                     NALRGIVALAANDVWAVGSAANAGGTPETLIEHWNGTAWSIVSSPNQGTSGNVLQAVS
                     ATGTGDVWAAGESGGSAGAQTLVLHWNGTAWSIVPSPNQGTGANTLYGIAAVAADDVW
                     AAGTYADAAAVNRTLVLHYTTVCSTPTATATVAASPTATPPPPATATPGGPPSATATP
                     GGPTATPVASATPCPIAFTDVPPSQPFYAYIRCLACRGIVSGYSDNTFRPGNDVTRGQ
                     LAKFVSNAAGYADAIPATQQTFSDVPPADPFWLFIERAALHGVISGYSDGTFHPTANV
                     TRGQAAKFIANSAGYTDAIPATQQSFTDVLPVDTFWLFIERVKLHDVISGYTCGA"
BASE COUNT          344 a          939 c          714 g          291 t
ORIGIN      
        1 ttcgaccatg tggcgccgcc cgccgcgccc atcccgcccg ccgacgcgct ggccggcaac
       61 atcgacgggc tgcgcggctt ccgcgtgccc tgcctgctca tctcgccctg gtcgccgcgc
      121 gccaccgtgg cccacaacgt ctatgaccac acctcgatcc tcaagctgat cgagtggcgc
      181 tgggatctcc cgccgctgac cgtgcgcgac gcgagcgcca acaacctggc gacggcgctc
      241 gacttctcgc agtcgaacct ggcggcgccc acgcccaccg tgccgcccgg cccgttcggc
      301 ggcgcctgcc ccgcgggcga gtccggcccc gacgacgaat ggcagatgct gcgcgacctg
      361 gcgcagaagt acggctggcc catttacggc ccccgcccgg ttctctcagg aaaggaacac
      421 actcgatgat gcaatcgccc actctggcgc ccgcggcccg gcgcatcccg cgcccgctcc
      481 tgttcgcgct ccttgccggg ctggctttcg ccatggccgg gctggtgggc acgggcagcg
      541 ccgcgctctc gcccgccccg gtccccgccg cgctcgccgc cgcgccgctc ggctgcccgc
      601 ccggcttcgt catcaccgcc agccccaata tcagcaacag cctcaacacg ttctacgcgg
      661 tcgcggcgcg cgccgccggc gatgtctggg ccgtgggcaa ctacgacaac ggcggcatcc
      721 tgcaaacgct gaccgcgcac tacgacggcg cggcctggac cgccgtgccc agccccaacc
      781 agggcagcac caacaacacg ctgcaaggcg tggccgccct cgcgcccgac gatgcctggg
      841 ccgtcggcgg ctacggcgaa gccatcaccc agagccagac gctgatcgag cactggaacg
      901 gcagcgcctg gagcctggcg accccgccgg cggtatcggg tgccaacggc ctgctggccg
      961 tctccgccgt tgcgcccaac gacgtgtggg ccgtgggcag cgcggccaac atcatcggcg
     1021 cgccgcagac ctttatcgcg cactggaacg gcagcgcctg gagccacatc gccagcccca
     1081 acgggaccgc tctcggcctc aacgtcctga ccagcgtgtc ggcgcgcgcc gccgacgacg
     1141 tgtgggcggc gggcttctac acggatctca gcggcgtgag ttacacgctg atcgagcatt
     1201 ggaacggcac cgcctggagc gtgatcgaca gtccggagcg cggcaccggc gacaacgccc
     1261 tgcgcggcat cgtggcgctg gccgccaatg atgtctgggc cgtgggcagc gccgccaacg
     1321 ccggcggcac gcccgaaacc ctgatcgagc actggaacgg caccgcctgg agcatcgtgt
     1381 ccagccctaa ccagggcacc agcggcaacg tgttgcaggc cgtcagcgcc accggcaccg
     1441 gcgatgtctg ggctgccggc gaaagcggcg gcagcgcggg cgcgcagacc ctcgtgctgc
     1501 actggaacgg cacggcctgg agcatcgtgc ccagccccaa ccagggcacc ggcgccaaca
     1561 cgctctacgg catcgccgcc gtcgccgccg acgatgtctg ggccgccggc acctacgccg
     1621 atgccgccgc cgtcaaccgc acgctggtcc tgcactatac cacggtctgc agcaccccca
     1681 ccgccacggc cacggtcgcc gcctcgccca cggccacgcc cccgccgccc gccaccgcca
     1741 cgccgggtgg cccgccatcc gcgacggcca cgccgggcgg gccaaccgcc acgccggtcg
     1801 cctcggccac gccctgcccc atcgcgttca ccgacgtgcc gcccagccag cccttctacg
     1861 cctacatccg ctgcctggcc tgccgcggca tcgtcagcgg ctacagcgac aacaccttcc
     1921 gccccggcaa cgatgtgacg cgcggccaac tggcgaagtt cgtgtccaac gccgcgggct
     1981 acgccgacgc catcccggcc acgcagcaaa ccttcagcga cgtgccgccc gccgacccgt
     2041 tctggctgtt catcgagcgc gccgcgctcc acggcgtcat cagcggctac agcgacggca
     2101 ccttccaccc caccgcgaac gtgacccgcg gccaggcggc caagttcatc gccaacagcg
     2161 cgggctacac cgacgccatc ccggccacgc agcagtcgtt caccgatgtg ctgccggtcg
     2221 atacgttctg gctgttcatc gagcgggtca agctgcacga cgtgatcagc ggctacacct
     2281 gcggcgcg
//
LOCUS       DASUYC010000243         2159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1408512, whole genome shotgun sequence.
ACCESSION   DASUYC010000243 DASUYC010000000
VERSION     DASUYC010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408512"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..474)
                     /locus_tag="VF276_03960"
     CDS             complement(109..474)
                     /locus_tag="VF276_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX6606050.1"
                     /translation="MPRERNEAWEHHLAMDIMPDAYDEEEQVMSWYYYLEEKLHFPFK
                     ARCIKARLKSPLEVGEIVQVIGMPGEDEIDHEMYVKIRWQKRDLAVPLIQLEGIDVDE
                     DTQEGISDWHYWVEQGYQF"
     gene            complement(674..2119)
                     /gene="guaB"
                     /locus_tag="VF276_03965"
     CDS             complement(674..2119)
                     /gene="guaB"
                     /locus_tag="VF276_03965"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX6606051.1"
                     /translation="MDEIPLSLSYDDVLLVPQRTAVRSRKQVDVSAQLSRHVRLQIPV
                     VSANMDTVTESTMAIAMARAGGIGIIHRFLPVEQQAAEVRRVKRAENFVIDQPYACRP
                     TDSIAHAQDLMHDYDVGTLMVVDNAGILAGMVGTRDVQFARDRTRPVESVMRPLDRLV
                     VGPPDVSTDKAAEIFAEQKIDKLPLVDTHGRLVGLITARDMLRISENPGAAKDRRGRL
                     LVGAAIGVIGDYMDRAAALLAEDADVLVIDIAHGHSEQVIEVAKEVRARWPQVELIAG
                     NVATAEGTLDLIAAGVDAVKVGVGPGSACTTRIVAGAGVGQFTAVWECARAARAHNIP
                     IIADGGIKASGDLTKAMAAGAHTVMIGGMLAGTDESPGRPLTRRGRRYKHYRGMASLA
                     ATVDRRKRERLDPGADDEWVSDVVPEGVEALVAYRGSAHEVLTQLVGGLRSGMSYCGA
                     FTLDEMYEKARFTRITSAGLRESHPHDVEFD"
BASE COUNT          331 a          758 c          673 g          397 t
ORIGIN      
        1 gtggaccccg ccaaggtcat cgaaggcagc ttcccgctgc tggacttcgt gcaaacggcg
       61 gtcgcgcgct aataaggggt tgcggcaggg cgggcggcga tccgtgcgct agaactggta
      121 gccctgctcg acccagtaat gccagtcgga gataccctcc tgggtatcct cgtccacgtc
      181 gatcccttcc aactgtatca ggggtacggc cagatcgcgc ttctgccagc gaatcttcac
      241 atacatttcg tgatcgatct cgtcttctcc cggcatgccg atcacctgga cgatttcacc
      301 gacctccaac ggcgatttga ggcgggcctt gatgcagcgc gccttaaagg ggaagtgcag
      361 cttttcttcc aggtagtagt accagctcat cacctgctct tcctcgtcgt aagcatcggg
      421 catgatgtcc atggcgaggt gatgttccca ggcttcattc cgttcgcggg gcatggcgct
      481 tcctcctccg caactcccgg catggtggca cggcaatccg ccgaataggg ctgcgggcgg
      541 gccggtttca aaccggcccc tacaaccgcg ggcgcactga ggccggcgat ctgcctgatt
      601 caccgatcac cgaccaccga ctactgacca ctgatcactg atcactgacc actgaccact
      661 gaccactcgt cccctaatcg aactccacgt cgtgcgggtg gctttcgcgc agcccggcgg
      721 aggtgatgcg ggtgaagcgg gctttctcgt acatttcgtc cagggtgaag gcgccgcaat
      781 agctcatgcc gctgcgcagc ccgcccacca gttgcgtcaa cacctcgtgg gcgctgccgc
      841 gataggcgac cagcgcctcc acgccttcgg gcaccacgtc gctgacccac tcgtcgtcgg
      901 cgccggggtc caggcgctcg cgcttgcggc ggtctaccgt ggcggccagc gaggccatgc
      961 cgcgatagtg cttgtagcgc cgcccgcggc gggtcagcgg gcgaccgggg ctttcgtcgg
     1021 ttccggccag catcccgccg atcatcacgg tgtgcgcacc cgccgccatg gctttggtca
     1081 ggtcgcccga tgccttgatg ccgccgtcgg cgatgatcgg gatgttgtgc gcccgcgccg
     1141 cccgcgcgca ttcccagacc gccgtgaact ggcccacccc ggcccccgcc acgatgcgcg
     1201 tggtgcaggc cgagccaggt cccacgccca ccttcaccgc gtccaccccg gcggcgatca
     1261 ggtcgagcgt gccctcggcg gtggcgacgt tcccggcgat caattcgacc tgcggccagc
     1321 gggcgcgcac ctccttcgct acttcgatca cctgctcgct gtggccgtgc gcgatatcga
     1381 tcaccagcac gtcggcgtcc tccgccagca gcgctgccgc tcggtccata taatcgccga
     1441 tcacgccgat agcggccccg accagcagcc gcccgcgccg gtccttggcc gcgcccggat
     1501 tctcgctgat gcgtagcatg tcgcgcgccg tgatcaggcc caccagccgg ccatgcgtat
     1561 ccaccagggg cagcttatcg atcttttgct cggcgaagat ctcggcggct ttgtccgtgc
     1621 tcacgtcggg cgggccgacc acgagccggt cgagcgggcg catcacgctc tccacgggcc
     1681 gggtgcggtc gcgggcgaat tgcacgtcgc gcgtgcccac catgccggcc aggatacccg
     1741 cgttgtccac gaccatcagc gtgcccacat cgtagtcgtg cataaggtcc tgcgcgtggg
     1801 caatcgagtc ggtggggcgg caggcatagg gctggtcgat cacgaagttc tcggcgcgct
     1861 tcacgcggcg cacctcggcg gcctgctgct ccaccggcaa gaagcggtgg atgatgccga
     1921 tgcccccggc gcgcgccata gcaatcgcca tggtcgactc ggtcacggtg tccatgttgg
     1981 cactgaccac cgggatctgc agccgcacat ggcgcgaaag ctgcgcgctc acgtccacct
     2041 gcttgcgcga gcgcacggcg gtccgttgcg gcaccagcaa cacatcgtca tacgacaggg
     2101 acaggggtat ttcgtccatt cgggtctcct tggtcagtgg tcagtggtca gtggtcagt
//
LOCUS       DASUYC010000244         3988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_484252,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000244 DASUYC010000000
VERSION     DASUYC010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484252"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="VF276_03970"
     CDS             complement(<1..889)
                     /locus_tag="VF276_03970"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6606052.1"
                     /translation="MVTTLQQPAPAGIATGQPVIDIEGLQVSYGAVQAVRNVSLQVRQ
                     GEIFGLLGPNGAGKTTTLAAIEGLVQPQAGRVIVLGRDIRTQAREIKRRLGISLQTTA
                     FFDSLRVWELVRFYAGLYDVMLSKAQVLDLLAGFELAEKANARAEELSGGQQQRLALV
                     LAIANDPEIVILDEPTTGLDPQARRHVWDSIRQIRARGCTVLLTTHYMEEAQELCHRV
                     AIIDRGEIIALDTPGGLINNLHADSLITATVRLPEDRVRALPDVTGAHYEGDKLTVRT
                     GNAQETVLGLQQLAVEQRQV"
     gene            complement(1084..2316)
                     /locus_tag="VF276_03975"
     CDS             complement(1084..2316)
                     /locus_tag="VF276_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013192402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6606053.1"
                     /translation="MPDVEKIDCCIVGGGPAGAVLALLLARQGVPVLLLEEHKDFDRD
                     FRGDTIHPSVLQVMDEIGLAGRLLQLPHTEMHTMSLETGAGTITPVDFRRLKTHYPYI
                     MMVPQVDFLNFVTKEAACYPQFHLRMNARVEELIEEGDVIRGVRYRGPDGRHEVRARL
                     TVGADGRFSRLRKLAGLAPIASSPPMDVLWFRLPRRPGDAEEAGVRFGAGRLGILLNR
                     FDYWQVGYVIAKGTYQPLRAAGIAAFRAQVAELFPELSDRVDAIRDWKDISLLSVESS
                     RLARWYRPGLLLIGDAAHVMTPVGGVGINYAIQDAVVAANVLGPRFRQGRLRLRDLAA
                     VQRRREWPTRIIQAAQATVQERLLGEALRSSGPFHLPPIARILLGIPLLRNLPARFIA
                     FGVWPVHVRGGRAGSPRA"
     gene            complement(2561..3832)
                     /locus_tag="VF276_03980"
     CDS             complement(2561..3832)
                     /locus_tag="VF276_03980"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEX6606054.1"
                     /translation="MANATLAADAASTARPAPDWAAVRADFPILRQEVHGYPLCFLDN
                     TATAQKPEPVLAAMDSYYRTTNANIHRGVYQMSEQATALYEDARARIAAFINAQSSRE
                     IIYTRNTTEAINLVAATWGRANVGPGDAILYTAMEHHSNLVPWQLLAQDRGATLRYIP
                     IDDAGRLVLDDLDTLLDGVKIVAVTYASNVLGTINPVAEITEAAHRHGAVVLIDGAQA
                     VPHMPVDVQALGCDFLAFSSHKMLGPTGIGCLYGRRSLLEAMPPYQGGGDMIRRVTLE
                     RSTWNDLPWKFEAGTPAIAEAIGLGAAVDYLSALGMDNVRAHEQQLVAYALDRLATVP
                     GLRVLGPPAAERGGVAAFSLGEIHPHDIASLLDQRGIAIRAGHHCCQPIMDYYDVPAL
                     ARASFYIYNTEADVDRLVEGLHFVREVFGSE"
BASE COUNT          620 a         1292 c         1399 g          677 t
ORIGIN      
        1 gcacctggcg ctgctcgacc gcgagttgtt gcagccccag gacggtctcc tgggcgttgc
       61 cggtgcgcac ggtcagctta tcgccttcgt agtgggcgcc ggtgacgtcg ggcagagcac
      121 gcacccggtc ttccggcagg cgcaccgtgg cggtgatcag gctgtcggcg tgcagattgt
      181 tgatcaggcc gccgggtgta tccagggcga tgatctcgcc gcggtcgatg atggcgaccc
      241 ggtggcagag ttcctgggcc tcctccatgt agtgcgtggt cagcagcacc gtgcagcccc
      301 tggcgcggat ctgccggatg ctgtcccaga cgtggcgccg ggcctgcgga tcgaggccgg
      361 tggtcggctc gtccaggatg acgatctccg ggtcgttggc gatcgccagg accagggcca
      421 ggcgctgctg ctgcccgccg ctgagttcct cggcccgtgc gttcgctttc tcggccagct
      481 cgaacccggc cagcagatcg agcacctgcg ccttgctcag catcacgtcg tagagaccgg
      541 cgtagaaccg gaccagttcc cagacccgca gcgagtcgaa gaacgctgtg gtttgcaggc
      601 tgatgcccag gcggcgcttg atctctcgcg cctgcgtgcg gatgtcgcga cccaggacga
      661 tgacgcgccc ggcctgcggc tgcaccaggc cctcgatggc tgccagcgtg gtggtcttgc
      721 ccgcgccgtt cggccccagc agcccgaaga tctcgccctg ccgcacctgc aagctcacgt
      781 tgcgcacggc ctgcaccgcg ccgtaggata cctgcaagcc ttcgatgtcg ataaccggct
      841 gcccggtggc aattcccgcg ggcgccggtt gttgtaatgt agtgaccata cttcctccca
      901 ttgcgacata cgtgtcgttt tcgatacagg tctatcttag caggcaaccg cggattctct
      961 tagcggagga agtaatgacc aggccgggag gaaagtcaaa caggcactgt gactttagtc
     1021 acaatgcctg taggtccagc gccgtaggct gtctaggcgg gcggttcttc cgctagtgtg
     1081 ctgtcaggcc cgcggcgagc cggctcgtcc gccgcgcaca tggaccggcc agacgccgaa
     1141 ggcgatgaac cgcgccggga gattgcgcag caacgggatg cccagcagga tgcgggcaat
     1201 gggcggcagg tggaacgggc cgctgctgcg tagcgcctcg cccagcaggc gctcctggac
     1261 ggtggcctgt gccgcctgga tgatccgcgt gggccattcg cgccggcgtt gcaccgccgc
     1321 caggtcgcgc aggcgcagcc ggccctggcg gaagcgcggc cccagcacgt tggccgccac
     1381 cacggcgtcc tggatggcgt agttgatgcc cacgccgccg accggcgtca tcacatgcgc
     1441 ggcgtcgccg atgagcagga ggccgggccg gtaccaacgc gccagccggc tcgactcgac
     1501 ggacagcagg gagatgtcct tccagtcccg gatggcatcc acccggtcgc tcagttcggg
     1561 gaacagttcg gccacctggg cgcggaacgc cgcgatgccg gcggcgcgaa gcggctgata
     1621 ggtgcctttg gcgatgacgt agcccacctg ccagtagtcg aaccggttga gcaggatgcc
     1681 cagccgcccg gcgccgaagc gcactcccgc ctcctcggcg tcaccggggc ggcggggcag
     1741 gcggaaccag agcacgtcca tgggcggaga actggcgatg ggcgccagcc cggccagctt
     1801 gcgcagccgc gagaagcgcc cgtcagcccc cacggtgagg cgggcgcgca cctcatgtcg
     1861 cccatccggc ccgcggtagc gcacaccgcg gatcacgtca ccctcctcga ttagttcttc
     1921 cacgcgggca ttcatccgca ggtggaactg cgggtagcat gccgcctctt tggtgacgaa
     1981 gttgaggaaa tcgacctggg gcaccatcat gatgtagggg tagtgggttt tcagccgccg
     2041 gaagtccacc ggcgtgatcg tcccggcgcc ggtctccagg ctcatcgtgt gcatctcggt
     2101 gtggggcagt tgcagcaggc gcccggccag cccgatctcg tccatgacct gcaataccga
     2161 gggatgaatg gtgtcgccgc ggaagtcgcg gtcgaagtcc ttgtgctcct ccagcagcag
     2221 cacgggcaca ccctggcgcg ccagcagcag ggccagcacg gcgcccgccg gcccgccgcc
     2281 tacaatacaa cagtcgattt tctccacgtc aggcacgcct gcctccttcg ccggcttcgt
     2341 ctgcgtctgc atagccctac ctctcgcttc attaatcgaa cgattgatta attacaagcc
     2401 gattatagat ccgattccgg cgcatgtcaa gaggatggcg ggaaccgcta tggaaacgca
     2461 ccgcgaaagc ggaaggacgc gaagaaccgg actaatttaa atcctcttcg cgttcttcgc
     2521 gtcttcgcgg tgcgaaacgt caccggccgg ctagcgcgga ctattcgctg ccgaagactt
     2581 cgcggacgaa gtgcaggccc tcgaccaggc ggtccacgtc ggcctcggtg ttgtagatgt
     2641 agaaggaagc gcgggccagg gcgggcacgt cgtagtagtc catgatcggc tggcagcagt
     2701 ggtgcccggc gcggatggcg atgccgcgct ggtcgagcag gctggcgatg tcgtgcgggt
     2761 ggatctcgcc caggctgaag gcggcgaccc cgccgcgctc ggcggcgggc gggccgagga
     2821 cgcgcaggcc gggcacggtg gcgagccggt ccagcgcgta ggccaccagt tgctgctcgt
     2881 gggcgcggac gttgtccatg cccagcgcgc tcaggtaatc cacggcggcg cccaggccga
     2941 tggcctcggc gatggccggg gtgcccgcct cgaacttcca gggcaggtcg ttccaggtcg
     3001 agcgctccag cgtgacccgg cggatcatgt cgccgccgcc ctggtagggc ggcatggcct
     3061 ccagcagcga gcgccgcccg tagaggcagc cgatgccggt ggggccgagc attttgtgcg
     3121 acgagaaggc caggaagtcg cagcccagcg cctgcacgtc caccggcata tggggcacgg
     3181 cctgcgcgcc gtcgatgagg accacggcac cgtggcggtg ggcggcctcg gtaatctcgg
     3241 ccaccgggtt gatcgtgccc agcacattgc tggcgtaggt cacggcgacg atcttcacgc
     3301 cgtcgagcag cgtgtcgaga tcgtcgagca ccagccggcc cgcgtcgtcg atggggatgt
     3361 agcgcagagt cgcgccgcgg tcctgggcaa gcaactgcca gggcaccagg ttgctgtggt
     3421 gctccatggc ggtgtagagg atggcgtcgc ccggcccgac gttggcccgg ccccaggtgg
     3481 cggccaccag gttgattgcc tcggtggtgt tgcgcgtgta gatgatctcg cggctcgact
     3541 gggcgttgat gaacgcggcg atgcgggcgc gcgcgtcctc gtagagcgcg gtggcctgct
     3601 cgctcatctg gtagacgccg cggtggatgt tggcgttggt ggtgcggtaa taggagtcca
     3661 tggcggccag caccggttcg ggcttttgcg ccgtggccgt gttgtcgagg aagcagagcg
     3721 ggtagccgtg cacctcctgg cgcagaatgg ggaagtcggc acgcaccgcc gcccaatcgg
     3781 gcgcggggcg cgctgtggat gcggcatccg ccgccagggt tgcatttgcc atcgctgtgc
     3841 cctcttacgt cgctgtcagc tatcagccgt cagccgtcag gtattagccg tcaggcccat
     3901 accctgttat atcggcattg tcgcaggaaa gcggatctgg tcgggatggc gaacgagatc
     3961 cgccgggcag gggccggttt gcaaccgg
//
LOCUS       DASUYC010000245         2737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_357601,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000245 DASUYC010000000
VERSION     DASUYC010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357601"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VF276_03985"
     CDS             <1..272
                     /locus_tag="VF276_03985"
                     /inference="COORDINATES: protein motif:HMM:NF014445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HEX6606055.1"
                     /translation="TYLLQWEAMRWARSRGCAHYDFWGIPDTPEAEAPEAGANQNVRQ
                     GLWGVYRFKQGFGGQEVDYTGAWDYVYRPLAYAVYRRLLARRVEG"
     gene            703..1653
                     /locus_tag="VF276_03990"
     CDS             703..1653
                     /locus_tag="VF276_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606056.1"
                     /translation="MRRGVIAALLVVLAGLAVLVAPGVRPAPLAAPTLTGRIAYASGG
                     NIWIWRNGAHQFTNSGHDGSPDLTPDGQRLTYVRYDDSFSDVLVQATAGGDAQFLTNN
                     RPVAETGSLEYVQQALWALNPAWAPDGSRIAWASDRGTDAPVLWIMTATGDNQHSLQT
                     SPPNPPVERLHWSPDGSAIVATSQGSGKDEIWSLDLDSGVWKEVAAPTDGAYDPAWSP
                     DGTRVAYAARTGRQTDIWVVPVDGSAPPAQLTQMGRARAPVFSPDGKQIAFLAEKDDR
                     FQMFVIDVREENGTLRAGSPQQVTSQDAGLDAPSGLSWSR"
     gene            complement(1762..2718)
                     /locus_tag="VF276_03995"
     CDS             complement(1762..2718)
                     /locus_tag="VF276_03995"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013630787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX6606057.1"
                     /translation="MDEILVFGGSGSPQLTQRICAYLGIAPGKNEVLRFSEGNLFVRI
                     LENVRGRPVYLVQSTAFPANDNFMELLFWIDAFRRASAESVTVVMPYFSYAKGDKKDE
                     PRVSIRARVCADAIEAAGADRIVTMDLHAPQIQGFFHIPVDDLYALPLLGDAVKAKGW
                     PNLIVVSPDAGFAKKAREYASYLGTSLAIADKERVAHDERATVLEIIGDVRGKTALIV
                     DDFTISAGTLVSVADKLIDRGADAVYAAITHAVFAPGSMERLDASPIRTLLISDTVET
                     QPVPISAKVETVSVAPLFGEAIRRIHNHESISVLFPQRTGSP"
BASE COUNT          454 a          933 c          914 g          436 t
ORIGIN      
        1 ccacctatct gctccagtgg gaggcgatgc gctgggcgcg cagccgcggc tgcgcgcact
       61 acgatttctg gggcatcccc gacacgcccg aagccgaggc gccggaggcc ggggcgaacc
      121 agaacgtgcg ccaggggctg tggggcgtct atcgcttcaa gcagggcttc ggtgggcaag
      181 aggtcgatta cacgggcgcc tgggactatg tctaccgccc gctggcctac gccgtctatc
      241 gccgcctgct ggcccgccgc gtggagggct gacccgttgc agttgtaggg gccggtttga
      301 aaccggcccg cgcatgccac ccactatcgt tccccgctgt cgcgtatgct gccgctgtag
      361 gggccggttt caaaccggcc tggtgggttt acccgcatga taggggtaga ttcccaggct
      421 ggttgcattg tataatggcg atacggcgct gatagtccgc cctggcgatt cggcccggcg
      481 gcaggccggt ttgaaaccgg cccctacagc gcgaaacggc ggcaggccgg tttgaaaccg
      541 gcccctaccg cgcgaaccgg cggtgggatg ctattctgac cactgaccac tgaccactga
      601 ccactgacca ctgattcggg acggcgaaca cgcgtaaagc agcgggggat acgagaccat
      661 gcaccacagg aagcagttgc acggtgccgg gcggagggcg cgatgcgccg cggcgtaatc
      721 gcggcgctgc tggtcgtgct ggccgggctc gcagtgctgg tcgcgcccgg cgtgcggccc
      781 gcgcccctgg ccgcgcccac gctcaccggg cgcatcgcct acgccagcgg cggcaatatc
      841 tggatctggc gcaacggcgc ccaccagttc accaacagcg gccacgacgg ctcgcccgac
      901 ctcacccccg acggccagcg cctgacctat gtgcgctatg acgacagctt ctccgacgtg
      961 ctggtgcagg ccacggcggg cggcgatgcc cagtttctga ccaacaatcg cccggtggcc
     1021 gagaccggca gcctggagta tgtgcagcag gcgctatggg ccttgaatcc ggcctgggca
     1081 cccgacggca gccgcatcgc ctgggcgtcg gacaggggca cggacgcccc cgtgctctgg
     1141 atcatgaccg cgaccggcga caaccagcat tcgttgcaga ccagcccgcc caatcccccc
     1201 gtcgagcggc tacactggtc gccggacggc agcgcgatcg tcgccacgag ccagggcagc
     1261 ggcaaggacg agatctggag cctggacctc gacagcgggg tctggaagga agtggccgcg
     1321 cccaccgacg gcgcctatga cccggcctgg tcgcccgacg gcacgcgcgt ggcctacgcc
     1381 gcgcgcaccg ggcggcagac cgacatctgg gtcgtgccgg tggatggctc ggcgcccccc
     1441 gcgcagttaa cgcagatggg gcgcgcccgc gccccggtct tctcgcccga cggcaagcag
     1501 atcgcgttcc tggccgaaaa ggacgaccgc ttccagatgt tcgtgatcga cgtgcgggag
     1561 gagaacggca cgctacgcgc cggcagcccg caacaggtca ccagccagga cgccggcctc
     1621 gacgccccgt cgggcctctc ctggagccgc tagccgggcg ctgtgtagtt atcttgtcat
     1681 tctgagcgga gcgaagaatc tcgtctcgta gcccaaacag cgagactctt cgctgcgctc
     1741 agagtgacag agttgggacg gtcagggcga tccggtccgc tgcggaaaca gcacgctgat
     1801 gctctcgtgg ttgtggatgc ggcggatagc ttcgccaaac agcggggcca cggagaccgt
     1861 ctcgaccttc gccgagatgg gtacgggttg cgtttccacg gtatcggaga tcagcaaagt
     1921 gcggatcggg ctggcatcca ggcgctccat cgaccccggc gcgaacaccg cgtgtgtgat
     1981 cgcggcgtag accgcatccg ccccgcggtc gatcagcttg tcggccacgc tcaccagcgt
     2041 ccccgccgag atggtgaagt cgtcgacgat cagcgcggtt ttgccccgca catcgccgat
     2101 gatctcaagc acggtggccc gctcgtcgtg ggccacccgc tccttgtcgg caatcgctaa
     2161 cgatgtgccc aggtaggatg cgtattcgcg cgccttcttg gcgaaccccg cgtcgggtga
     2221 caccacgatc aggttgggcc accctttggc cttgacggca tcccccagca gcggcagcgc
     2281 gtagaggtcg tccacgggaa tatgaaagaa gccctggatt tgcggggcgt gcaggtccat
     2341 cgtgacgatg cggtcggcgc ccgccgcctc gatcgcatcc gcgcagaccc gcgcccggat
     2401 cgagacccgc ggttcatcct tcttgtcgcc cttggcgtag ctgaagtacg gcatcaccac
     2461 cgtgaccgac tcggcgctgg cgcgccggaa ggcgtcgatc cagaataaga gctccatgaa
     2521 gttgtcgttg gccgggaacg ccgtggactg gaccaggtag accgggcgcc cgcgcacgtt
     2581 ctccagaatc cgcacaaaga ggttgccttc ggagaagcgc aggacctcgt ttttgcccgg
     2641 cgcgatgccg aggtacgcgc agatcctctg cgtgagctgc gggctgcccg aaccgccgaa
     2701 caccaggatt tcgtccacgt cccgcctccc gctacct
//
LOCUS       DASUYC010000246         2687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1439497, whole genome shotgun sequence.
ACCESSION   DASUYC010000246 DASUYC010000000
VERSION     DASUYC010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439497"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..922
                     /gene="rimI"
                     /locus_tag="VF276_04000"
     CDS             212..922
                     /gene="rimI"
                     /locus_tag="VF276_04000"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEX6606058.1"
                     /translation="MPYIIEQMQMADVPEVSAVEKECFASPWPASAYRRELRNPQANR
                     YVVARWVHPSARRRPPTVWPPAGSPLRNRLGEIFPALFPPVEPPGSPYPIAGFAGLWL
                     MGEEAHVTTIGVASAHRGRHVGELLFLTMIDIALEQRAAWLTLEVRVSNRVAQNLYQK
                     YGLTIAGTRKHYYSDDGEDAYLMWSEPLQSPAFRDRLSRLRAEFLAGMSRQDTEESAG
                     RLQLPASGKQSAPEGSGP"
     gene            957..1319
                     /locus_tag="VF276_04005"
                     /pseudo
     CDS             957..1319
                     /locus_tag="VF276_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641590.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            1560..1898
                     /locus_tag="VF276_04010"
                     /pseudo
     CDS             1560..1898
                     /locus_tag="VF276_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641590.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
BASE COUNT          445 a          998 c          924 g          320 t
ORIGIN      
        1 gcaactgagc acgtatgatg aagcccggaa ggcttggaaa tgggcagatc gttccggtga
       61 ttcagaactg atttcattaa ttctcaccga gcagggaaca cgagcgttaa cagaataact
      121 gtccgatata ctgcaagcag caaggactag ccggttggcc gccagtgatc accgcccacg
      181 gatcactgac cactgaccac cgatcactcg tatgccgtat atcatcgaac aaatgcagat
      241 ggccgatgtg ccggaggtct cggcggtgga gaaggagtgc ttcgccagcc cgtggccggc
      301 cagcgcctac cgccgggagt tgcgcaaccc gcaggccaac cgctacgtgg tggcccgctg
      361 ggtccacccc agcgcgcgcc ggcgcccgcc caccgtctgg ccgcccgccg ggtcgcccct
      421 gcgcaaccgg ctgggcgaga tcttcccggc gctcttcccg ccggtcgaac cgcccggcag
      481 cccctacccc atcgccgggt tcgcgggcct gtggctcatg ggcgaggaag cacacgtcac
      541 cactatcggc gtggcgtccg cccaccgggg ccgccacgtg ggcgagttgc tgttcctgac
      601 catgatcgac attgccctgg agcagcgcgc cgcctggctg accctcgaag tgcgcgtcag
      661 caatcgcgtg gcccagaact tgtaccagaa gtatgggctg accatcgccg gcacgcgcaa
      721 gcattattac agcgacgacg gcgaagatgc ctacctgatg tggagcgagc cgttgcagtc
      781 gcccgcgttt cgcgaccggt tgagccgcct gcgggccgag tttctggcgg gcatgagccg
      841 ccaggacacc gaggaaagcg ccgggcggct gcaactgccg gcatcgggga agcaatccgc
      901 cccggaaggg agcggcccgt gagccgcggc gggcggcggg aaggagccga gcgccggtga
      961 atatcctggc tatcgaaacg tcgtgtgatg aaacatccgt cgccattgtg cgcgacggac
     1021 gcgaagtgct ggcgaatatc atcgcctcgc agatcggcct gcaccagcgc ttcggcggcg
     1081 tggtgcccga actggcctcg cgccagcaca tcgtcacgct gatccccacg ctggaagagg
     1141 caatgcacca tgcgggcgtg gactgggacg gtatcgacgc catcgccgtc acctgcgggc
     1201 cggggctggc cggggcgctg ctggtcggcc tcaacgcggc caaggctatc gccttcgccc
     1261 gcaacaaacc gctgatcgcc gtcaaccacc tcgaagcgca tatctattcc aactggatca
     1321 tcccggagac gctggagcgc gaagggccgc cgcccccgcg cgaacacccg cccgccgccg
     1381 agcaaacccg cgcgggcgcg gcgttgcggg cgctgggcgt ggaaccgccg cgccgggccg
     1441 gcgaaaccga tctgccgccc ggcccccctg cgggcgggcg cccgcaatat actccgccgc
     1501 cgccgaagca ggaggcgccc cggcccgaca tcgaccgggt ggcgccgacc gatccggcct
     1561 tcccgctgct ctgcctggtc gtctccggcg ggcacaccga gctgatctat atgagcgggc
     1621 acggccagta cagcctgctg ggcaagacgg tggacgacgc ggcgggcgag gcgttcgaca
     1681 aagtggctcg cgtgctggga ctgccctacc ccggcggccc ggccatccag cgcgtcgccg
     1741 ccttcggcga tcccgacgcc atcgcgttgc cgcgcacggt gctgcggggc agcgccgact
     1801 tcagcttcag cggcctgaag acggcggtgc tccgccaggc ccagaactac ggcccgccga
     1861 tcacgccgga ggagcaggcc gccgagcgcg aagccgccga acgcgcccgc cgtgaggagg
     1921 aagcgcgcat cgccgccgag gaccgcacgc gccgcgaagc cgaggagcgc acgcgccggg
     1981 aagaagcagc gcgccaggaa gccgaggaac gggagcgccg cgaggccgaa gcgcgcgcgc
     2041 gctgggaggc cgaggaacag gcccgccgcg aagccgcgga agagcgccgc gccgccgctg
     2101 ccgcccgccg ggccgaggaa gagcgtgcac ttcaggagga agccgatcgt cgcgcggcgg
     2161 aagcccgcac ccgccgcccc gaaccggccc caccggcgga cgaccgcccg cgccgtgccg
     2221 atacgtccga gcgcccgtct atctcgatcc tgggcggcgg gcgcgcgacc ccgcgcccgg
     2281 cgccgcccga atctgcgccc gaagcgccgc cggcatccgc tggcccgccc gcagaggcgg
     2341 aacccgagcc tccgggctct aaagaggccc cggccgcgcc gcgcaaatcg cgcagttccg
     2401 gcctgatccg cgtcacgccg gagagcatcc gcccggaggc cgggccgccg cctggcgccg
     2461 tggagcactt cgaccgcgaa gcggccccgc cgatcactgt gcgccccgaa cgggacacgc
     2521 cccgcgtgct gggcaagacc gagcaggaag aggtgccgcc gcccgccttt ctggccgagc
     2581 gtgggggcgg caccccgccc acgcctatgc gccgccgccc cgcgacgccg ggcgaacccg
     2641 acgtgcccgc gttcctgcgc gggcgccccc aacccggcga accggcc
//
LOCUS       DASUYC010000247         2018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1883589, whole genome shotgun sequence.
ACCESSION   DASUYC010000247 DASUYC010000000
VERSION     DASUYC010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1883589"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VF276_04015"
     CDS             complement(<1..247)
                     /locus_tag="VF276_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEX6606059.1"
                     /translation="MADRLLLGIDIGTSGSKAILLAADGAVVATATAEYPMYTPRPLW
                     AEQNPTDWWAATVQAIRQVLAAPGVEARQVAGVGLTGQ"
     gene            563..1147
                     /locus_tag="VF276_04020"
     CDS             563..1147
                     /locus_tag="VF276_04020"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX6606060.1"
                     /translation="MEYFVAPERLETPTFTVRSYMPGDGVLLNPAVAGSYEHLRTFMP
                     WARPDPSPESSEEFCRRGRGEYLLNQNFVMGIFSPGGDRLLGGTGFHLRGQSLDQRKA
                     DIGMWIRADAAGQGLGTAVLVAMLRWGFDAWPWLRLTWHCDTRNVASARTAEKAGMLL
                     EGTHRSDTFGGASTERHDIHVFAALRDEWTPPAR"
     gene            complement(1157..1462)
                     /locus_tag="VF276_04025"
     CDS             complement(1157..1462)
                     /locus_tag="VF276_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606061.1"
                     /translation="MLEEEWTVAVGADLTRAAQVIAAACAAEGLRLTLDSTLAKYPGC
                     RHWHYKRDRQRGTLEITLWPARRRLWITVHAGRTGDSIADCLPRIRRAVEAGLDAPG"
     gene            complement(1474..>2018)
                     /locus_tag="VF276_04030"
     CDS             complement(1474..>2018)
                     /locus_tag="VF276_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606062.1"
                     /translation="DAGAGFGKPADYGIPAEDVAFRAYRLYDRAQLRHVDLFVWNPAT
                     GAYEPSETHIDFNFGPPETPAMPEDAILLYYQAINAKDYAGAYKYLGPQMQQQLPFDQ
                     FQAGFAKTVRVWVSSLEPEGDSTPVEKLADGTYTDRVEIVSEDQGANGARVRQTFAGT
                     WTVRVTNGVARLESAQIKAK"
BASE COUNT          282 a          671 c          709 g          356 t
ORIGIN      
        1 tctgcccggt cagcccgacg cccgccacct gccgggcctc gacgccgggc gcggcaagca
       61 cctgccggat cgcctgcacg gtcgccgccc accagtcggt ggggttctgt tcggcccaga
      121 gcgggcgcgg tgtatacatc gggtactcgg ccgtggcggt ggcgaccacg gcgccgtcgg
      181 cggccagcag gatcgctttg ctgccggacg tgccgatatc gattcccagc aggaggcggt
      241 cagccatgag ttcggttctc cttgcctgtt tcttgattag ccgtggcggt ggcggggctt
      301 gctcagcaag cagagtgccc tcgcctctgc cgccggcagg cccaccggct cgaggtggca
      361 gacgggccac cacgccgggt ttctcggtga tcgctatgcc gggtccggcc cgagtctgcc
      421 ggcaccgcaa ttatgctaga gaaaccgaag attttcaaat gcgcccggcg cggccactgt
      481 cccggcggcg cttggtggga tgcgagggct atggtagaat ggagcgccga tcagacagca
      541 ccactagcag gaggagggca ggatggagta ttttgtggcc ccggagcgcc tggagacgcc
      601 cacgttcacg gttcgatcct atatgccggg agatggggta ctcctcaatc cagccgtcgc
      661 cggctcgtat gagcatctac gcaccttcat gccctgggca cggcccgatc cctccccgga
      721 gagttccgag gagttctgcc gccggggccg tggcgagtat ttgctgaacc agaatttcgt
      781 gatggggatt ttcagccccg gcggggaccg gttgctgggg ggcaccgggt tccacctgcg
      841 cgggcagagc ctggatcagc gcaaggccga tatcggcatg tggattcgcg ccgacgccgc
      901 ggggcagggg ttgggcaccg ccgtgctggt ggcgatgctg cgctggggct tcgacgcctg
      961 gccctggctg cgcctcacct ggcattgcga cacacgcaac gtcgccagcg cgcgcacggc
     1021 ggagaaggcc ggcatgttac tcgaaggcac gcatcgctcc gataccttcg gaggagcctc
     1081 gaccgaacgc cacgacatac acgttttcgc cgccttgcgc gacgagtgga ctccgcccgc
     1141 ccggtagggg ccggtttcag cccggcgcgt ccaggcccgc ctccactgcc cgccggatgc
     1201 ggggcaggca atcggcgatc gagtcgccgg tgcggcccgc gtggacggtg atccagaggc
     1261 ggcggcgggc gggccagagc gtgatttcga gcgtgccgcg ctgccggtcg cgtttgtagt
     1321 gccagtgccg gcagccgggg tatttcgcca gcgtgctgtc cagcgtcagc cgcaagccct
     1381 ccgccgcgca ggctgcggcg atcacctgcg cggcgcgggt caggtcggcg cccacggcca
     1441 ccgtccactc ttcttcaagc accgggcggc ctgctatttc gccttgatct gggcgctttc
     1501 gagccgggct acaccgtttg tgacgcgcac cgtccaggtg ccggcgaagg tctggcgcac
     1561 ccgcgcgccg ttcgcgccct ggtcctcgct gacgatctcc acccggtcgg tgtacgtgcc
     1621 gtcggccagc ttttcgaccg gcgtgctgtc gccctccggc tccaggctgc tgacccagac
     1681 gcgcaccgtc ttggcgaagc cggcctggaa ctggtcgaac ggcagttgct gctgcatctg
     1741 cggccccagg tacttgtacg ctccggcgta gtccttcgca ttgatcgcct gatagtagag
     1801 caagatcgca tcttcgggca tggccggcgt ttccggcggg ccgaagttga agtcgatgtg
     1861 cgtttcgctc ggctcatagg cgcccgtggc cgggttccag acgaacaggt ccacgtggcg
     1921 gagttgggcg cggtcgtaga gccggtaggc ccggaaggcg acatcctcgg cgggaatgcc
     1981 gtagtcggcg ggcttgccga agcccgcgcc ggcatcgc
//
LOCUS       DASUYC010000248         3289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_935119,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000248 DASUYC010000000
VERSION     DASUYC010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935119"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            371..>3289
                     /locus_tag="VF276_04035"
     CDS             371..>3289
                     /locus_tag="VF276_04035"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6606063.1"
                     /translation="MADREPFKAWFKQRRKAFDLTQGELAQQAGCSIYTVQRIEEGVL
                     RPSRQLGELLAAALAIPPEERAAFVRWARTGAGPAVLAGVSVALADATASTATPAGNG
                     RGDPAVSPSPVVLGVPANPYKGLRAFQEADAPYFFGRESLTRQLQARLADDEALSRFL
                     AVVGPSGSGKSSVVRAGLVPALRRHLLPGGLSPVVADLVPGTHPLEELEAALLRVAAN
                     PPASLMEQLRADERGLARAVKRVLPSDDQTELVLVIDQFEELFTLVADEEVRQDFLDS
                     LFSAVADPRSRLRVVITLRADFYDRPLLYLPSSELLGRRTEVVGPLTAQELYAAITGP
                     AERAGLELESRLVATILQDVGDQPGTLPLLQYALTELYERRDGHRLTLAAYQAGGGVF
                     GSLAGRAERLYAALSEAEQTAARQLFLRLVTLGEGVEDTRRRVPMSELVSASRAEDAL
                     HRVLDLYGRYRMLAFDREPRTNEPTVEVAHEALLHSWGRLRDWLRDSRELLQTERRLL
                     AAADEWQAAGQDASFLASGARLAQFAGLAAQSGQPQTPALTSEEQAYLAASLETQRRQ
                     TEAEEARRTREVALQKRSANRLRYLVAALALFLAVATGLALLAEGRQQEAQANLIHSE
                     ALRLAAEANSLLQTGGPAELAGLLSVRAIRTQYSPQGDAALEAAARLAYPVRRFAGYT
                     DTVWISTFSPDGKILLAQSDTATREWDVATGRQLAVLPFSGNNLAFSPDGRAIAGPQN
                     DGTIDIWDTQAMTVTQVLTAAPAPGGKPAALLEAFYSPDGRLLAANGEDKTVRVWDVA
                     GGRQLQVFTLESKLTSALAWSPDSKFVVAGAEDNVARVWDVQSGRLVHSFNKGLAPGA
                     GPGVVAVAYSPDGKTLATDGPRNNALLWDVATGDQLRDFAGHTGPVWSVAFAADGKTL
                     LTGAGDATARLWDPATGQPLQVFRGPPAMVEGTVAAAISPDGTHVATNYFTGEVILWD
                     A"
BASE COUNT          504 a         1108 c         1163 g          514 t
ORIGIN      
        1 ggcgcatcgg ccagcgcgtg ggcgcccggc agggcggcga ccactgccag caggctcacg
       61 agcagggtac caatccgtgc cccaaactta gcgaagcgca tctgcttacc tcctgtatct
      121 ccctcatttg ctgccggatc ccctcttccg ccgcgccacg gaacggagcc gcgcccgaga
      181 acccgctccg gggatagccg cgccactcct gggcggctaa cccccgcggt gcagccatta
      241 tcgcaaggtc cgccgccggg cgtacagggt tttgtgatat aataggtcac acagttattc
      301 gtacaaatac tcatacagcc tcagaattac accctgtttg ccccttatat ccgtagaaag
      361 ttaaagcatg atggcggatc gggagccctt caaggcgtgg tttaagcagc gccgcaaagc
      421 cttcgacctc acccagggcg agcttgccca gcaggccggc tgctcgatct atacggtgca
      481 gcggatcgag gagggcgtgt tgcgcccgtc gcggcagtta ggggaactgc tggccgccgc
      541 gctggccatc ccgccggagg agcgcgcggc tttcgtgcgc tgggcccgga cgggggcagg
      601 gccggcggtg ctcgccggag ttagcgtggc cctggccgat gccacagcgt ccaccgcgac
      661 cccagcaggt aatgggcgcg gcgatcccgc cgtatctccc tcgcctgtcg tcctgggcgt
      721 gcccgcgaac ccgtacaaag gcttgcgcgc cttccaggag gccgatgcgc cctatttctt
      781 cgggcgggaa tcgctcaccc ggcaattgca ggcacgtctg gccgatgacg aggcgctgtc
      841 acgcttcctg gcggttgtcg ggccgtcggg cagcggcaaa tcgagcgtgg tgcgcgcggg
      901 attggtgccc gcgttgcgcc ggcacctgtt gccgggcggg ctatcccccg tggtggccga
      961 tctcgtgccg ggcacacacc cgctggaaga actagaagcg gccttgctgc gggtcgccgc
     1021 caatccgccg gccagcctga tggaacagtt gcgtgccgac gagcgcgggc tggcgcgcgc
     1081 cgttaaacgc gtgctgccca gcgacgacca gaccgaactg gtgctggtga tcgaccagtt
     1141 cgaagagctg ttcaccctcg tcgccgacga ggaggtgcgc caggattttc tcgacagcct
     1201 cttcagtgcc gtcgccgatc cgcgcagccg cttacgggtg gtgatcacac tgcgcgccga
     1261 cttctacgat cgccccctgt tgtatctgcc gtctagcgaa ttgctgggcc gccgcaccga
     1321 ggtggtgggg ccgctcaccg cgcaggagct ctacgcggcg atcaccggtc ccgccgagcg
     1381 ggccgggctg gagttggaga gccgcctggt cgccaccatt cttcaggacg tgggcgacca
     1441 gccgggcacg ctgccgctgc tgcaatacgc tctcaccgag ttgtacgagc ggcgcgacgg
     1501 gcaccggctg acgctggcgg cgtaccaggc gggcgggggc gtgttcgggt cgctggccgg
     1561 gcgggccgag cgtctctacg cggcgctgag cgaagcggag cagacggcgg cacggcagct
     1621 ttttctgcgg ctggtgacgc tgggcgaagg agtggaggac acgaggcgtc gcgtgccgat
     1681 gtccgaactg gtttcggcgt cccgcgctga ggatgcgctg caccgcgtcc tagacctgta
     1741 cgggcgatac cgcatgctgg cgttcgaccg cgagccccgg accaacgaac cgacggtgga
     1801 ggtggcccac gaggccctgc tgcatagctg ggggcggctg cgcgactggt tgcgcgacag
     1861 ccgggaactg ttgcagacgg agcggcgctt gctggccgcg gcggacgagt ggcaggccgc
     1921 ggggcaggat gcgagtttcc tggcgagcgg ggcgcggttg gcgcagtttg ccggactcgc
     1981 cgcgcagtcc ggccaaccgc agaccccggc gttgacgagt gaggagcaag catacctggc
     2041 cgccagcctg gaaactcaac ggcggcagac ggaggcggag gaggcgcgcc ggacgcgaga
     2101 agtcgcttta cagaagcgat ccgcaaaccg gctgcgctat ctggtggcgg cgctcgcgct
     2161 cttcctggcg gtggcgaccg ggctggcgct gctggccgag ggccggcaac aggaagccca
     2221 ggccaacctc atccattccg aggcgttgcg gctggccgcc gaagccaata gcctgctgca
     2281 gaccgggggc cccgccgaac tggccggctt gctgagcgtg cgcgcgatcc gtacccaata
     2341 ctcgccgcag ggcgacgccg cgctggaggc tgccgcccgg ctcgcctacc ccgtgcgccg
     2401 cttcgccggc tatacggata ctgtctggat ctccaccttc tcgcccgacg gcaagatctt
     2461 gctggcccag agcgacaccg cgacgcgcga gtgggatgtc gccacggggc gccaactcgc
     2521 cgtcctaccc tttagcggca acaatctggc tttctcgccc gacgggcggg cgattgccgg
     2581 cccgcagaac gacggcacca tcgacatctg ggatacccag gccatgaccg tcacccaggt
     2641 gctgaccgcc gcacctgccc ctggcgggaa gcccgcggcg ctgctggagg ccttctactc
     2701 gcccgacggg cgcctcctcg cggcgaacgg cgaggacaag acggtgcggg tgtgggacgt
     2761 ggccggcggg cggcagttgc aggttttcac gctggagagc aagttaacca gcgccctggc
     2821 ctggtcgccc gacagcaagt ttgtggtcgc cggggcggaa gataatgtgg cgcgggtctg
     2881 ggatgtgcaa agcgggcggc tggtgcatag cttcaacaaa gggctggcgc ccggcgcggg
     2941 gcccggcgtc gtcgccgtcg cctactcgcc ggacggcaag accctggcga ccgatgggcc
     3001 gcgcaacaac gcgctgctct gggatgtcgc caccggcgac cagctacgcg acttcgcggg
     3061 gcacacgggc ccggtctgga gcgtcgcctt tgcggcggac ggcaaaacgc tcctgaccgg
     3121 ggcgggcgat gcgacggctc gcctctggga ccccgcgacg gggcagccgt tgcaggtgtt
     3181 ccgcggcccg ccggccatgg tcgaaggcac cgtcgccgcc gccatctcgc ccgacggcac
     3241 gcatgtcgcc accaactact tcaccggcga ggtgatcctg tgggatgcc
//
LOCUS       DASUYC010000249         2807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_19893,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000249 DASUYC010000000
VERSION     DASUYC010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19893"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..940)
                     /locus_tag="VF276_04040"
     CDS             complement(146..940)
                     /locus_tag="VF276_04040"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HEX6606064.1"
                     /translation="MKRWQIATVGVALAVASVGVAGASFTGSPHPDGPVVVVPGTVVV
                     ANPNWQPSGPGPARPAPLPAAPALPAATGPVLFQADFAAADPAAWHAPLLRDSDLAPA
                     WTVRDGVLQQSGDEALNTRNDEAYYLTGAANWSDLVLDADVFATSGEGAGLVWNVQGD
                     NFYRVQLFPALPNNAPKARLELVQGGQVTVLAEADPAAYAGYPFDAWQQVRVVSSAGH
                     QQVWINGVAVFDVTNSVLSSGQVGLYAWADSGTRFDNVRVQRAVVR"
     gene            complement(1200..1508)
                     /locus_tag="VF276_04045"
     CDS             complement(1200..1508)
                     /locus_tag="VF276_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017297844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEX6606065.1"
                     /translation="MAFAETRYEHVVLDDTQTAYIAGTSMKVIELVSAWRAYGWSPEE
                     LHFQYPQLSLGQIHSALAYYWDHQAALDTAIDSQLAEADALRRSTPAVLLAGRVRRHS
                     "
     gene            complement(1725..>2807)
                     /locus_tag="VF276_04050"
     CDS             complement(1725..>2807)
                     /locus_tag="VF276_04050"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEX6606066.1"
                     /translation="KDVTTYYIHGRGTTFSTPELVFPSSWGLAVVRHPDIDVNGGYAH
                     IAANSNVSGSYENYYAYTQALGGSITPLPTATNTPHACIPGQFDDVPPANPFYRYITD
                     LVTRGAISGYSDCTFRWENNITRGQTAKVIMLANSDKYQIINPPMESFEDVPYGSTFY
                     TYIETAYRNGIISGYDCGGVGEPCDAQNRPYFRPNNQVTRGQLSKMASLARQWTLLNP
                     TTPTFTDVPQANPFYKEVETAYAHGIISGYNCGAPPAGVCDPQNRPYFIPFGTATRGQ
                     ASKIIDLSLYSNDITPTPVPPTATATSVPPTATSVPPTATVTGTPPTATATSVPPTAT
                     STSVPATATATELPATATSTPMVNVR"
BASE COUNT          454 a          804 c         1053 g          496 t
ORIGIN      
        1 aatcggtcgg gacggcctgg gccgccgccg gggtgcccgc cgccgccggg aagacagcga
       61 agcttgcgcc ggccacagtg agcatggaca ggaggctcgc ggctatcgcg aaaactcgtt
      121 tcatggaaag cagatctcct ctcggctagc gcacgaccgc gcgctgcacg cggacattgt
      181 cgaagcgggt gccgctgtcg gcccaggcat acagccccac ctggccgctg ctcaacacgc
      241 tgttggtgac atcaaacacg gccaccccgt tgatccagac ctgctggtgg ccggcgctgc
      301 tgaccacgcg cacctgctgc caggcgtcga agggatagcc ggcataggcg gcgggatcag
      361 cctcggccag caccgtcact tggccgccct ggacgagttc gagacgagcc ttgggggcgt
      421 tgttgggcag ggcggggaag agttggacgc ggtagaagtt gtcgccctgg acgttccaga
      481 ccaggccggc gccttctccg ctggtggcga agacatcggc gtcgaggacc agatcggacc
      541 agttggcggc gccggtgagg tagtaggcct cgtcgttgcg ggtgttgagg gcctcgtcgc
      601 cgctctgctg gaggacgccg tcgcggacgg tccaggcggg ggcgaggtcg gagtcgcgca
      661 acaggggcgc gtgccaggcg gcggggtcgg cggcggcgaa gtcggcctgg aagaggacgg
      721 ggccggtggc ggcgggcagg gcgggggcgg cggggagggg ggcggggcgg gcggggccgg
      781 ggccgctggg ctgccagttg ggattggcga cgacgacggt gccgggtaca accacgaccg
      841 ggccgtccgg gtgcggcgag ccggtgaacg aggccccggc aacgcccacg gaggcaacgg
      901 ccagcgccac gcccacggtg gcaatttgcc atctcttcat taggtactcc tctcgggatt
      961 tcttgccttg cgtgccatgg cacgcgcata tggcgcgccc tacagtgggt ggatggtggg
     1021 tatgatgtat cgggaaacga cggtgccctg cgctgtcacc atgggagcgg cccacaccga
     1081 cggagcgccg tcccgcgcgg gcgaagaacc gggaaggcca tcaatctcgc ggacgagatt
     1141 ctgacggaaa ttatagcact tctaagtagt ttatgtcaat gtgcctaacg ggagcgcaat
     1201 caggagtggc ggcgcacgcg accggccagc agcacggcag gtgtgctacg gcgcaacgca
     1261 tccgcctcgg caagttggct gtcgatggcg gtatcgagcg cggcttgatg gtcccagtaa
     1321 taggcaagcg cggagtggat ctggccaaga gacaactggg gatactggaa atggagttct
     1381 tcgggactcc acccgtaggc acgccaagcg ctaaccaact cgatgacctt catcgaggtg
     1441 ccagcgatgt aagccgtctg tgtgtcatcc agcacgacat gttcgtagcg ggtttcggca
     1501 aaggccatgg ttcgttcgtc gccctccgcg agtaacttac ggccggcaaa gggatccccg
     1561 ctgtgggcag gaaacccaat cgaatctcaa cctattatat catagcaccg ttgtgtacat
     1621 ctctgtgttg attctacacg ggtattagac caaacaggcc ggagcacatg ctccggcctg
     1681 tcgggcggtg agtaccgcgg gatgcccgcg cggggcggac ctgcttagcg cacgttcacc
     1741 atcggggtcg aggtggccgt ggcaggcagt tccgtcgcgg tcgcggtcgc cggcacactg
     1801 gtcgaggtgg ccgtgggcgg cacactggtc gccgtggctg tcggcggggt gcccgttacg
     1861 gtcgcggtcg gcggcacgct ggtagccgtg ggcggcacgc tggtcgcggt ggccgtcggc
     1921 gggaccggcg tcggcgtgat gtcgttcgag tagagcgaca ggtcgatgat cttcgaggcc
     1981 tggccgcggg tggcggtgcc gaacgggatg aagtacggcc ggttctgtgg gtcgcagacg
     2041 cccgccggcg gcgcgccgca gttgtagccg ctgatgatgc cgtgggcgta ggccgtctct
     2101 acttccttat agaacgggtt ggcctgcggc acgtcggtga aggtcggcgt ggtggggttc
     2161 agcagcgtcc actggcgggc caggctcgcc atcttggaca actggccgcg cgtgacctgg
     2221 ttgttgggcc ggaagtaggg ccggttctgc gcgtcgcagg gttcgccgac gccgccgcag
     2281 tcatagccgc tgatgatccc attgcgatag gcggtttcga tgtaagtgta gaaggtcgag
     2341 ccgtagggca cgtcctcgaa gctctccatc ggcgggttga tgatctggta cttgtcgctg
     2401 ttggcgagca tgatcacctt ggccgtctgg ccgcgggtga tgttgttctc ccagcggaag
     2461 gtgcagtcgc tgtagccgga gatggcgccg cgcgtcacca ggtcggtgat gtagcgatag
     2521 aacggattgg cgggcggcac gtcgtcgaac tggccgggga tgcaggcatg cggcgtgttc
     2581 gtcgcggtgg gcaacggcgt gatgctgccg ccgagggcct gggtgtaggc gtagtagttc
     2641 tcataggagc ccgacacgtt gctgttcgcc gcgatgtggg catagccgcc gttcacgtcg
     2701 atatccgggt ggcggacgac cgcgaggccc cacgagctag gaaagaccag ttccggtgtg
     2761 ctgaacgtcg tgccacggcc gtggatgtag taggtggtga cgtcctt
//
LOCUS       DASUYC010000250         2189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_735220,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000250 DASUYC010000000
VERSION     DASUYC010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735220"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VF276_04055"
     CDS             complement(<1..385)
                     /locus_tag="VF276_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606067.1"
                     /translation="MAEAPPDLNPAPVESAPPPAPAAEAPATGRRVVLRRRHLWVIAP
                     LALIWILQSLASIEPFDFWWNVTSGRIMFETGRFLADDVLVYTPVRQPYSNPQWGSQL
                     LFYAFYALWPGLLLAVRVAIITATYG"
     gene            complement(366..974)
                     /locus_tag="VF276_04060"
     CDS             complement(366..974)
                     /locus_tag="VF276_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1990 domain-containing protein"
                     /protein_id="HEX6606068.1"
                     /translation="MLQLTPPTDAQIRRLLADQRPRPYSYRAVGATRTRPPAWHTVDH
                     NRVRLGSGRAVWERAVAAVRRWEMFHLGWVRLYWPDTPIAAGSTVAVVSRQWGFWSVN
                     AARIVYVVCDDGPVARFGFAYGTLPHHVERGEERFCVEWRHADDSVWYDILAFSRPHH
                     PLLIPGYPVARIVQRRFARDSKRAMRRAVHAQEESARGRGAT"
     gene            complement(993..1880)
                     /locus_tag="VF276_04065"
     CDS             complement(993..1880)
                     /locus_tag="VF276_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6606069.1"
                     /translation="MPEIKFGWIAPVMGAEKTGWVPIVMSQQETILPVVAQHFDSVWI
                     YDHLYGLIDPADPWLECWTTLTWLAARFPTLRVGPIVLGLSYRNPALVAKMAATLQTL
                     SGGRLVLGIGAGWREDEHRAFGYPFPPAAVRRRQLDEALEIIRRMWTEPQPSFQGEYF
                     GIQDAYCRPQPPPPPIMIGSSGEAILPLVARRADWWDVWFWTAAEISAEAYRPKGDAL
                     NRAAEAIGRDPASIPRSISSTGCRLPRDSAQSQQWLDLLRPFVDLGVTRFLFDFGNPR
                     SPEPILRFAEEVIAPLNRV"
     gene            complement(1967..>2189)
                     /locus_tag="VF276_04070"
     CDS             complement(1967..>2189)
                     /locus_tag="VF276_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606070.1"
                     /translation="DFDPIQLMFWVNVLQGVLAPVLVAVLLLVGNNRRIMGVQRFGWA
                     TNLGLAVTALVMFAATALLFYGLLTGQGG"
BASE COUNT          365 a          745 c          782 g          297 t
ORIGIN      
        1 tgccgtaggt ggccgtgatg atcgccacgc gcacggccag caacagcccc ggccagagcg
       61 cgtagaaggc gtagaacagc agttgcgacc cccactgcgg gttgctgtag ggctggcgca
      121 cgggggtata gaccaggaca tcgtcggcca ggaagcggcc cgtctcgaac atgatccgcc
      181 cgctggtcac gttccaccag aagtcgaacg gctcgatact ggccaggctc tggaggatcc
      241 agatcagcgc cagcggggcg atcacccaga ggtggcggcg gcgcagcacg acgcggcgcc
      301 cggtcgccgg ggcctcggcg gcaggggcgg gtggcggggc gctctcgacg ggcgcggggt
      361 tcagatcagg tggcgcctcg gccacgggca gactcctctt gtgcatggac ggcgcgccgc
      421 atggcccgct tggagtcgcg ggcgaagcgg cgctggacga tccgcgctac cggatagccc
      481 ggaatgagca gcggatggtg gggccgtgag aaggctaata tatcatacca taccgagtca
      541 tcggcgtggc gccattcgac gcagaatcgc tcctcgccgc gctcgacatg atgaggcagc
      601 gtgccgtagg cgaagccgaa gcgcgccacc ggcccgtcat cgcagacgac gtagacgatg
      661 cgggcggcgt tgaccgacca gaagccccac tgccggctga ccacggcgac ggtggacccg
      721 gcggcaatcg gcgtatcggg ccagtagagg cgaacccagc ccaggtggaa catttcccag
      781 cggcgcacgg cggccacggc ccgctcccac accgcgcgcc cgctgcccag ccgcacccgg
      841 ttgtggtcga cggtgtgcca tgcgggcggc ctggtgcgcg tggcgcccac ggcgcggtac
      901 gaatacggtc gcgggcgctg atcggcgagc aggcggcgga tctgggcgtc agtgggcggc
      961 gtgagttgca gcatcaagca ggcggcccgg cctcagaccc gattcagcgg cgcgatcact
     1021 tcctcggcga agcgcagaat cggctccggc gagcgcgggt tgccgaagtc gaacaggaag
     1081 cgggtgacgc ccagatccac gaagggtcgc aacaggtcca gccactgctg cgattgcgcc
     1141 gagtcgcgcg gcaggcggca gccggtgctc gaaatgctgc gcgggatgct cgccgggtcg
     1201 cggccgatgg cctcggcggc gcggttcagc gcgtcccctt tgggccggta cgcctccgcg
     1261 ctgatctccg ccgccgtcca gaaccagaca tcccaccagt cggcgcgccg ggccaccagc
     1321 ggcaggatgg cttcgccgga gctaccgatc ataatcggcg ggggcggcgg ctgcgggcgg
     1381 cagtaggcgt cctggatccc gaaatactcg ccctggaagc tcggttgcgg ctcggtccac
     1441 atgcggcgga tgatctccag ggcttcgtcc agttgccggc ggcgcacggc ggcgggcggg
     1501 aacggatagc cgaaggcgcg gtgctcgtcc tcgcgccagc ctgcgccgat gcccagcacc
     1561 agccgcccgc cgctgagggt ctgcaacgtg gcggccatct tcgccacgag cgccgggttg
     1621 cggtagctca ggccgagcac gatggggccg acacgcagcg tgggaaagcg cgccgccagc
     1681 caggtcagcg tggtccagca ttcgagccag gggtccgccg ggtcgatcag cccgtagagg
     1741 tggtcgtaga tccagaccga gtcgaagtgc tgtgccacaa ccggcaggat cgtctcctgc
     1801 tggctcatga cgataggcac ccagccggtc ttctcggcgc ccatgaccgg cgcgatccag
     1861 ccgaacttga tctccggcat gctttcctcc attgctgccc cgcgcgggcc ggtttgaaac
     1921 cggcccctac accgccggcg ggatctgccc tacccgacgc acaggattaa cctccctgcc
     1981 cggtcagcag gccgtagaac agcagcgccg tcgcggcgaa catgaccagc gccgtcaccg
     2041 ccaggcccag gttggtcgcc cagccgaagc gctgcacgcc catgatccgg cggttgttgc
     2101 cgaccagcag caggacggcg acgagcaccg gcgccagcac gccctgcagg acgttgaccc
     2161 agaacatcaa ctggatcggg tcgaagtcg
//
LOCUS       DASUYC010000251         2045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_30439,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000251 DASUYC010000000
VERSION     DASUYC010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30439"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1582)
                     /locus_tag="VF276_04075"
     CDS             complement(<1..1582)
                     /locus_tag="VF276_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004047674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEX6606071.1"
                     /translation="MDKALRNTLRTTVTQARTLLEGEIGDLLEGQFGVHRDGRVEPAA
                     AVAHLSADDQTYRAQILANLRHIEAAGYKPADAVEQLTREAAFTHLNRLCAYKLLERR
                     KLIREAVSRGPKSNGFQFYLSDHPDEEALSTSGQQEVVYRHFLEWLGGTLSAEIGVLF
                     APHDPANRLFPRGQVLGKVLALLNAEELTPVWDSDETIGWVYQYFTPKELRDRARKES
                     AAPRNSYELAFRNQFFTPRYVVEFLTDNTLGRLWYEMRGGQTRLAAQCRYLVRRHDTV
                     FLDKPMSEEGLIAVRWLQGDTVPEPDPTILAHTVAGHTRHPQGDKTDAWMHHMQAEVL
                     AGRAAEQTSQDLLDLLFLLARAERHISGEGVDAGFPDEVAAILPVLRERRPRPGAESA
                     DGAGGPRPTIHTVAYREPQDPRSLRVIDPACGSGHFLLYAYDLLETIYDEAYDDETLG
                     PALHRAYPDRDAYRREVPRLILAENLHGIDIDLRATQIAALALWLRAQRSWAAQGLKR
                     EARPPVRRTNLVPAAPMPG"
     gene            complement(1588..>2045)
                     /locus_tag="VF276_04080"
     CDS             complement(1588..>2045)
                     /locus_tag="VF276_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606072.1"
                     /translation="DKELQDYFKTFTFSLAETLRILEDPEGAVLFIVQKMLSFMNIRY
                     LSSIKGVTVVATLLWVRDPWVVLGRRLNRNPTDLEQVIKRTTDRRNDIVHRADRSEKD
                     PEGVIQPVSLAFAQQAVDTIKHVCLALDELVEERMAEFRGEIQSRAQEN"
BASE COUNT          298 a          678 c          706 g          363 t
ORIGIN      
        1 cgccgggcat gggcgcggcg ggcaccagat tggtgcggcg cacgggcggg cgggcctcgc
       61 gcttcagccc ctgggcggcc caactgcgct gcgcccgcaa ccacagcgcc agcgcggcga
      121 tctgcgtggc ccgcaggtcg atgtcgatgc cgtgcaggtt ttcggccagg atcagccggg
      181 gcacctcgcg gcgataggcg tcgcggtcgg ggtaggcgcg gtgcagggcc ggccccagcg
      241 tctcgtcgtc atacgcctcg tcgtaaatgg tctccagcag gtcgtaggca tagagcaaga
      301 agtggccgct gccgcaggcc gggtcgatca cgcgcaggct gcgcgggtcc tgtggctcgc
      361 ggtaggcgac ggtgtggatc gtgggccgcg ggccgccggc gccgtccgcg gactcggcgc
      421 cagggcgcgg gcggcgctcc cgcagcacgg gtaggatagc cgccacctcg tcggggaagc
      481 ccgcgtcaac gccttcgccg cttatatgcc gctcggcgcg cgccagcagg aacagcaaat
      541 cgagcagatc ctggctcgtc tgctccgccg cccgccccgc cagcacctcg gcttgcatgt
      601 gatgcatcca ggcatccgtc ttatcgccct gggggtggcg ggtgtggccg gcgacggtgt
      661 gggccaggat ggtggggtcg ggttcgggta cggtgtcgcc ttgcagccag cgcacggcga
      721 tcagcccctc ctcgctcatg ggcttgtcca ggaagaccgt gtcatggcgg cgcaccaggt
      781 agcggcactg ggcggccagg cgggtctggc cgccgcgcat ctcataccag agccggccca
      841 gcgtgttgtc ggtcaggaac tcgaccacgt agcgcggggt gaagaactgg ttgcggaagg
      901 ccagttcgta gctgttgcgg ggcgcggcgc tctccttgcg cgcccggtcg cgcagctcct
      961 tgggcgtgaa atactggtac acccagccga tggtctcgtc ggagtcccac accggggtca
     1021 gctcctcagc gttgagcagg gccagcacct tgcccagcac ctgccctcgg ggaaacagcc
     1081 ggttggccgg gtcgtgtggc gcgaacagca cgccgatctc cgccgacagc gtgccgccca
     1141 gccattcaag aaagtgccgg taaaccactt cctgctgccc gctggtcgac aacgcttctt
     1201 catcggggtg gtcggacagg tagaactgga agccgttgga tttcggcccc cggctcaccg
     1261 cctcgcggat cagcttgcgc cgttccagca gcttataggc gcacagccgg ttgaggtggg
     1321 tgaaggcagc ctcgcgggtc agttgctcga cggcatccgc cggcttgtag cccgccgcct
     1381 cgatatggcg caggttggcg aggatctgcg cgcggtaggt ctggtcgtcc gcgctcaggt
     1441 gggccacggc cgccgccggc tccacccgcc cgtcgcggtg gacgccgaac tgcccctcca
     1501 acaggtcgcc gatctcccct tccagcaggg tgcgcgcctg ggtcacggta gtgcgcagag
     1561 tgttgcgcag ggctttatcc atgccgtcta gttctcctgt gcccggctct gtatctcgcc
     1621 gcggaactcg gccatgcgtt cctccaccag ttcgtcgagc gccaggcaca catgtttgat
     1681 ggtgtctacc gcctgctggg caaacgccag gctcaccggc tggatgacgc cttcggggtc
     1741 tttctccgac cggtcggccc ggtggacaat atcgtttcgc cggtcggtgg tgcgcttgat
     1801 cacctgctcc aggtcggtcg ggttgcggtt gagccgccgg cccaacacca cccagggatc
     1861 gcgcacccac aacagggtcg ccaccacggt tacacctttg atggaactga ggtagcggat
     1921 attcatgaag ctgagcatct tctggacaat gaacaggacc gccccctccg ggtcttcaag
     1981 gatgcgcagc gtttccgcca ggctgaaggt aaaggtcttg aaatagtcct gtagctcttt
     2041 atcgg
//
LOCUS       DASUYC010000252         2140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1707732, whole genome shotgun sequence.
ACCESSION   DASUYC010000252 DASUYC010000000
VERSION     DASUYC010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1707732"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="VF276_04085"
     CDS             complement(<1..868)
                     /locus_tag="VF276_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008302483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX6606073.1"
                     /translation="MIYSGSAISVSLLSDGIAELKFDLANESVNKFDRATLTELAAAT
                     DAIKASKDVKGVVVTSGKGVFIVGADITEFGALFSGGEGQIAEWGIGANKVFSAFEDL
                     DVPKVVAINGFALGGGFEMCLACEYRVMSTAAKVGLPEVKLGIMPGFGGTVRLMRVIG
                     VDNAAEWIAAGAEKRPDVALKDGAVDAVVAPELLKDAAIALVREAIAGKLDWRAKRQE
                     KLEPIKLNNMEQMMAFNTATAMIAGQAGPNYPAPKLAIQNMQAQANSKRDDAIAIEAQ
                     HFAKAAVTPQANA"
     gene            complement(1186..>2140)
                     /locus_tag="VF276_04090"
     CDS             complement(1186..>2140)
                     /locus_tag="VF276_04090"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6606074.1"
                     /translation="ILDYSKIEAGRLELEALDFAPRELCEQVVALLQPQAEERGLALA
                     TDLASALPPRLRGDHERLRQVLVNLLGNAIKFTAAGQVVLRCQVVGSDARGRARLQFE
                     VEDSGIGMDAATLARLFQPFTQADVSTTRRFGGPGLGLAICKRLVDLMGGSVEVESQP
                     GRGSTFRVQLPFEVVTGPPPAAAAGPALPPVPARPRGRVLVAEDNPVNQLLAVEMLKR
                     LGCRADVVGNGKEAVEALRQLPYDAVFMDCHMPEMDGLEASRRIRTEEAGARHIPIIA
                     FTASALKGERERCLEAGMDDYLSKPLRLVDLAAAVERWLPS"
BASE COUNT          319 a          723 c          759 g          339 t
ORIGIN      
        1 gggcattggc ctgcggggtg acggcggcct tggcgaagtg ctgggcctcg atggcgatgg
       61 cgtcgtcgcg cttgctgttg gcctgggcct gcatgttctg gatggccagc ttcggcgccg
      121 gataattcgg accggcctgg ccggcgatca tcgcggtggc cgtgttgaag gccatcatct
      181 gttccatgtt gttgagcttg atcggctcca gcttttcctg gcgcttggcg cgccagtcga
      241 gcttgccggc gatcgcttcc ctgaccaggg cgatggcggc gtccttcagc agttcgggag
      301 cgaccacggc gtcgacggcg ccgtccttga gggccacgtc cgggcgcttc tcggcgccgg
      361 cggcgatcca ctcggcggcg ttgtccacgc cgatcacgcg catcaggcgc acggtgccgc
      421 caaagcccgg catgatgccc agcttcactt ccggcaggcc gaccttggcg gcggtggaca
      481 tgacgcggta ttcgcaggcc aggcacattt caaagccgcc gccgagggcg aagccgttga
      541 tggccacgac cttgggcacg tccaggtctt cgaaggcgga gaacaccttg ttggcgccaa
      601 tgccccattc ggcgatctgg ccttcgccac cggagaacag cgcaccgaat tcggtgatgt
      661 cggcgccgac gatgaagacg cccttgccgg aggtgacgac gacgcccttg acgtccttgc
      721 tcgccttgat cgcgtcggtc gccgcggcca gctcggtgag cgtggcgcgg tcgaacttgt
      781 tcacggattc gttggcgaga tcgaatttga gctcagcgat gccgtcgctg agcagcgaga
      841 cgctgatggc tgatcccgaa taaatcatga gtgatctcca tagtgggatg gtgagcgttt
      901 gcctgcacgg ttggccccgg gagaacaggg cggcaggtca ccgcagcgca ctgtgggaag
      961 ccggaagccc aactgcctga aaaaccacca agcgggcgtc cgggccggcg cacagggtgc
     1021 ccaagtgtat gcatcccaag gggttttgtt aatgaaaacg cggggggaag ggtgactgga
     1081 cggccacgtt gccagcgccc aatggcggta gcgggccgat cctggccgcg tggcggcagc
     1141 gcgaccgccc cctcccccgg gccggtcccc gccaggccgc cgggttcagg acggcagcca
     1201 gcgctccacc gcggcggcca ggtcgaccag gcgcagcggc ttggacaggt agtcgtccat
     1261 gcccgcctcc aggcagcgtt cgcgctcgcc cttcagggcg ctggcggtaa aggcgatgat
     1321 gggaatgtgg cgcgccccgg cctcttcggt ccggatgcgg cggctggcct ccagcccgtc
     1381 catctccggc atgtggcaat ccatgaagac ggcgtcgtag ggcagctggc gcagcgcctc
     1441 gaccgcctcc ttgccgttgc cgaccacatc ggcccggcag ccgaggcgct tcagcatctc
     1501 caccgccagg agctggttca ccggattgtc ctccgccacc agcacgcgtc cgcgcgggcg
     1561 cgccgggacc ggcggcagcg ccggtccggc cgccgccgcc ggcggcggac cggtcacgac
     1621 ctcgaagggc agctgcaccc ggaacgtcga gccccggccg ggctggctct cgacctcgac
     1681 gctcccgccc atgagatcca ccaggcgctt gcagatggcc aggccgaggc cggggccgcc
     1741 gaagcgccgg gtggtggaaa cgtcggcctg ggtaaagggc tggaacagcc gcgccagggt
     1801 ggcggcatcc atgccgatgc cgctgtcctc gacctcgaac tgcagccggg cgcggccacg
     1861 ggcgtcgctg ccgacgacct ggcagcgcag caccacctgc ccggcggcgg tgaacttgat
     1921 ggcgttgccg agcaggttca ccaggacctg gcgcaggcgt tcatggtcgc cgcgcaggcg
     1981 cgggggcaac gcggacgcga ggtcggtcgc cagcgccagg ccccgctctt cggcctgcgg
     2041 ctgcaggagc gcgaccacct gctcgcagag ctctcgcggc gcgaagtcca gcgcctccag
     2101 ctcgaggcgg ccggcttcga tcttggagta atccaggatg
//
LOCUS       DASUYC010000253         2100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_65845,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000253 DASUYC010000000
VERSION     DASUYC010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65845"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..>2100)
                     /locus_tag="VF276_04095"
     CDS             complement(79..>2100)
                     /locus_tag="VF276_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606075.1"
                     /translation="SALATVSVHAAPLQSGGAATQIPISGTGSPQTGEAPPSGEGDVT
                     QAEFVGEADGDEGPDAYSGNIIDRSLSHGTGHGASTTSGTKAKSNPTFNSGFEGLNFY
                     QQRYARSGNQFSIEPPDQALCVGGGYELEAVNDVLNIFNAAGQSVLPDNTATNIVSGH
                     PRNVNHAVDLNSFYGYAPAINRSTGIRGPELTDPTCLYDAATQRFFVVVLTLEVVPTT
                     GAFTHVNHLDLAVSQTADPTGAWNIYKTDVTNDGTNTGGVNPGPYLGDYPHIGADANG
                     FYITTNAYPWTANGFAGAQIYAFSKAQLAAGAAHVTMVHMDTSGMVNAPSDAGSTQPG
                     FTVWPAQSPGTQFNSANGGTEYFMSSNAADEATHPVAGFGGNYVSNQLVVWTLANTSS
                     LNTVSPALALSNRVLGVNTYAIPPRQKQPGNGSAPGTNAPQGHCINDTTTPTIAGVGC
                     WRLLFNPPGPAHNEVVSRPDSNDTRMQQVMYANGKLWGALDTALNPGGTAQRAGIAWF
                     IVNPNAGSVARQGYLGAAGYDFTYPAIGVTPSGRGVMAFTATGDAHYPSAAYAGIDAV
                     AGVGAWNIVPGGEGAAPHDGFTGYKAQVGNPPRTRWGDYGAAAVDGNAVWVASEYIAQ
                     TCDYAAWGGPFFVGGSGDNLLGTCGGASFGPGARGALGNWSTRVSKFTP"
BASE COUNT          282 a          702 c          744 g          372 t
ORIGIN      
        1 ggagttcgct tgcacactcg gcgtccggcg ccccccttcc cgccgcggga cggggggttg
       61 ggggtcaggt ggcgtgccct acggcgtgaa cttgctgacg cgcgtcgacc agttgccgag
      121 cgcgccgcgg gcgccgggtc caaaactggc gccgccgcat gtccctagca ggttgtcgcc
      181 ggagccgccc acgaagaacg ggccgcccca ggccgcgtag tcgcaggtct gggcgatata
      241 ctcgctggcg acccagaccg cgttgccgtc gaccgccgcc gcgccgtagt cgccccagcg
      301 cgtgcgcggc gggttgccca cctgcgcctt gtagccggtg aagccgtcgt gcggggccgc
      361 gccctcgcca ccggggacga tgttccaggc gcccacgccg gcgaccgcgt cgatacccgc
      421 gtaggccgcg ctcgggtagt gggcgtcgcc ggtggccgtg aacgccatca cgccgcgccc
      481 gctcggcgtg acgccgatgg ccgggtaggt gaagtcatag cccgcggccc ccagatagcc
      541 ttgccgggcc acactgcccg cattcgggtt gacgatgaac caggcgatgc cggcccgctg
      601 cgcggtccca ccggggttga gcgccgtatc gagcgcgccc cacagcttac cgttggcgta
      661 catcacctgc tgcatacgtg tgtcgttcga gtcgggccgc gagacgactt cgttgtgtgc
      721 cggtcccggc gggttaaaca acagccgcca gcagcccacc ccggcgatgg tcggggtcgt
      781 ggtgtcgttg atgcagtggc cctgcggcgc gttcgtcccc ggcgccgagc cgttgccggg
      841 ctgcttctgc cgcggcggaa tcgcgtacgt atttacgccc agcacccggt tactcagcgc
      901 gagcgccggc gacaccgtgt tcagcgacga ggtgttcgcc agggtccaca ccaccagttg
      961 gttggacaca tagttgccgc caaagccggc caccgggtgg gtcgcctcgt cggcggcgtt
     1021 cgagctcatg aagtactcgg tgccgccgtt ggcgctattg aactgggtgc ccggcgactg
     1081 ggccggccag acggtgaaac ccggctgggt cgaaccggcg tcgctcggcg cgttcaccat
     1141 gcccgacgtg tccatatgga ccatggtcac atgggccgcc ccggcggcga gctgcgcctt
     1201 ggagaaggcg tagatctgcg cgcccgcgaa gccgttggcc gtccacgggt acgcgttcgt
     1261 ggtgatgtag aagccgttgg cgtccgcgcc gatgtgcgga tagtcgccca ggtacgggcc
     1321 ggggttcacg ccgccggtat tcgtgccgtc gttggtgaca tcggtcttgt agatgttcca
     1381 ggccccggtc gggtccgccg tctggctaac cgcgaggtcg aggtggttta catgagtaaa
     1441 ggcgccggtc gtcggcacaa cttccagcgt aagcaccacc acgaagaagc gctgcgtagc
     1501 cgcgtcgtac aggcacgtgg ggtcggtgag ttcgggaccg cggatgcccg tgctgcggtt
     1561 gatcgccggt gcgtagccgt agaacgagtt caggtccacc gcgtggttca cattgcgcgg
     1621 atgccccgac acaatgttgg tggccgtgtt gtccggcagc accgactggc cggcggcatt
     1681 gaagatgttg agcacgtcgt tcacagcttc gagttcatag ccgcccccta cgcatagcgc
     1741 ctggtccggc ggctcgatcg agaactggtt gccgctgcgg gcgtagcgct gctggtagaa
     1801 gttcagcccc tcgaagccgc tgttgaacgt cgggttcgac ttggccttcg tgccgctggt
     1861 cgtcgaggcg ccgtggccgg tgccgtgcga gaggctgcgg tcgatgatgt tgccgctgta
     1921 tgcgtccggc ccttcgtcgc cgtcggcttc gccgacgaac tcggcctggg ttacgtcgcc
     1981 ctcgcccgac ggcggggcct cgcccgtctg tggcgagccc gtgccgctga tgggaatctg
     2041 cgtggccgcg ccaccactct gcaacggtgc cgcgtggacc gataccgtag ccagtgcgct
//
LOCUS       DASUYC010000254         5140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1405697, whole genome shotgun sequence.
ACCESSION   DASUYC010000254 DASUYC010000000
VERSION     DASUYC010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5140
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1405697"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="VF276_04100"
     CDS             <1..745
                     /locus_tag="VF276_04100"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6606076.1"
                     /translation="YRHREHGPRDTQIVRVPLLAFDRFIDDPALAGHIVDLNTYYDDV
                     RNGTLPAVAYIAPLGASEQPPGDVGAGQRFVKGLVDTLVKSDAWDSSAFILTYDYWGG
                     WYDHVAPPQVDAYGYSFRVPTLLVSPYVRKGAIDSTTYDVTSILKFIEDNYGLKPLAT
                     RDAQATSIAKAFNFSQAPRAPQIISLDPPAGTLAGPRRVVLELSYGGALVIAGLIIAW
                     AAFGARFGGRSVYIRSLSAKLPSRKDGKR"
     gene            742..>5140
                     /locus_tag="VF276_04105"
     CDS             742..>5140
                     /locus_tag="VF276_04105"
                     /inference="COORDINATES: protein motif:HMM:NF027952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain repeat protein"
                     /protein_id="HEX6606077.1"
                     /translation="MNYRLQLLRTPVLLLALALLTVLGARVAQAGQRPEKGSEHTTTV
                     LTIQLPVSHDSGRHAPVVAHLAKSTGEPIGGITIVFFVDSQRDGQANTDADGNATWRL
                     RTELNAGTHYIQAVLYQVGDLLPAQASTQLVVEGTKLDLQIQPALAAGGTRAVMTAHL
                     TSASGKPVEGGLITFAVDGTRAGEAATDGTGTAQWRFDGNLAGGNHTIQATFAGLPAF
                     TPSQASAGLAVGGTKLTIARVPAPGQDAGHAPVVAHLTTDAGTPINGASITFLSDNQP
                     NGQTATDAAGNAVWHPRTALGIGNHTVTAVFDGVQGYLPSRASLQIGVAATNLTIQAA
                     STAGQSGSRIPVVAHLTNAAGQPVAGASVDFLVDGARDGEALTDASGNAAWRLRADLA
                     AGSHKVEAVFDGQADSGPSRGSMQVTVAATKLVLQTSGAGVTARLTTGDGQPVAKALI
                     KFLVDGTADSATATDAAGNAEWRTRQTLAAGGHKVEAVFEGLPGLLGTQATVQLGIGG
                     TKLTVQMPSNAGQGGTRIPVVAHLTTAGGQPVAKTVVEFQVDGKHDGEALTDAAGNAT
                     WRLRDGLAAGNHRVTAVFAGSPALAGATATTQVAVGAARLEFRAGGQSAIIAHLSAGG
                     RPIAQMPIHFLVDGKEAGQVATNAAGDATWAPKGQLSAGNHKVDAVFRGAPGLLPAQI
                     TTQWAVGATTLTVRPVAATAAGGTRQQVEAHLVDAAGKPVVKATIQFLADGARGGEAA
                     TDANGIATWHIRRDLAAGAHKIDATFAGLPGLVASQASTQVEVGASKLALQQDPAAVG
                     GTRVPTVAHLTDAAGKPISGAVIEFQIDGVRDGEAATDANGNATWRVRQAPTEGSHKV
                     AAIFHGMPGLLGAEASTQFSVGPTTLAVRVVTDASQSGERAPVTAHLIDGAGKPIAGG
                     VVAILVDGKRDGEAATDAAGDAAWRIRADLTPGAHKIEAQFTGLPGLLPSTAATQLTV
                     AATRLTLDQVPLGAAVGDPFVMQARLSNADGTPLTDARVEFLVNGSRDGEARTDATGT
                     VNWRLRRLLSAGTYTITASFPGVPGLEPESRSTQATVQPKEIEIQTVPAVPGMQFQLD
                     DKTFTSDANGIVRMPVSEPRTYKLKPLPLDATKAGLRAEFVRWGDDIFTSERDLIVPA
                     RDRYQIGFNVSYLVNLTFADLGGRTVDPARVTSISLKSSNGKDTFTGQSTSRWLLGSG
                     VTRTAVGLQERKVQYALEEAIVGGSNVVNQSEQRFTPGTATSWPITLRFYAVHFVARD
                     ALFDFPMGKTIRLEYPDKHVQTVSLGTNAEATLDGLPRGPYQVSVTNVPGISPATPIA
                     LSQDQQARLLVLSYLDIGVALGVLGLIALGLLLVGRFQVVAKVRRRFGWEKLGVAPAH
                     RTVRLPEQERALLAATAPVAVDDHTPVNTTSRYAVPELMTYEVVNGSRNVERILLGDR
                     AWERTPNGEWRVSARAKDADPAADGVLDGV"
BASE COUNT          796 a         1919 c         1736 g          689 t
ORIGIN      
        1 ctaccgccac cgcgagcacg gcccgcgcga cacgcagatt gtgcgcgtgc cgctgctggc
       61 cttcgaccgc ttcatcgacg accccgcgct cgccgggcac atcgtcgatc ttaataccta
      121 ctatgacgat gtccgcaacg gcacgctgcc cgcggtggcc tacatcgccc cgctgggcgc
      181 cagcgagcaa ccgcccggcg atgtcggggc cggccagcgc ttcgtaaagg gcctggtcga
      241 tacgctcgtc aagagcgatg cctgggatag ctcggcgttc atcctcacct acgattactg
      301 gggtggctgg tacgaccatg tcgcgccgcc gcaggtcgat gcctatggct acagcttccg
      361 cgtgccgacc ctgctggtca gcccgtacgt gcgcaagggc gcgatcgaca gcacgaccta
      421 tgatgtgacc tcgatcctca agttcatcga ggacaattac gggctgaagc cgctcgccac
      481 ccgtgatgct caggcgacga gcatcgccaa ggcgttcaat ttctcccagg cgccccgcgc
      541 gccgcagatt atttcgctgg acccgcccgc gggcaccctg gccgggccgc gccgggtcgt
      601 tctggaactg tcctacggcg gtgcgctagt cattgccgga ctgattatcg cgtgggcggc
      661 gttcggggcg cggttcgggg ggcgctcggt ctacatccga tcgctctcgg ccaagctgcc
      721 ttcgaggaag gatggtaagc gatgaactat cgactgcaac tgctgcgtac ccctgtgttg
      781 ctgctcgcgc tggcgctgct gacggtgctc ggcgcccgcg tggcccaggc cggccagcgc
      841 cccgagaagg gcagtgagca tacgaccacg gtgctcacga tccaactgcc tgtgtcccac
      901 gacagcgggc ggcatgcgcc cgtggtggcc cacctggcga agagcaccgg cgaacccatc
      961 ggcggcatca ccatcgtctt cttcgtcgac tcccagcgcg acggccaggc taacaccgat
     1021 gccgacggga acgccacctg gcgcctgcgc accgaactca acgccgggac ccactatatc
     1081 caggcggtgt tgtaccaggt cggcgacctg ctgcccgccc aggcttcgac ccaactggtg
     1141 gtcgaaggca ccaagctgga cctgcagatc cagccggccc tggcggcggg cggcacccgc
     1201 gccgtgatga ccgcgcacct gaccagcgcg tccggcaagc cggtcgaggg cggcctgatc
     1261 accttcgcgg tggacggcac gcgcgccggg gaagccgcca ccgacggcac cggcacggcg
     1321 cagtggcgct tcgacggcaa cctggccggc ggcaaccaca ccatccaggc gaccttcgcc
     1381 gggctgcccg cgttcacgcc ctcgcaggcg tccgccgggc tggccgtcgg gggcaccaag
     1441 ctgaccatcg cccgcgtacc cgcaccgggt caggatgcag gccatgcccc cgtggtcgcc
     1501 cacctgacca ccgacgccgg cacgccgatc aacggtgcgt cgatcacctt cctcagcgat
     1561 aaccagccga acggccagac ggcgaccgat gcggcgggca atgccgtctg gcacccgcgc
     1621 accgcgctcg gcatcggcaa ccacaccgtg accgcggtct tcgacggcgt gcagggctac
     1681 ctgccctcgc gggcttcgct gcagatcggc gtcgcggcca ctaacctgac catccaggcg
     1741 gcctcgaccg ccggccagag cgggtcgcgc atccctgtcg tggcccacct gaccaatgcc
     1801 gcgggccagc ccgtggccgg ggccagcgtg gacttcctgg tggacggcgc ccgcgatggc
     1861 gaggcgctga ccgatgcgtc gggcaacgcg gcctggcgcc tgcgcgccga cctggccgcc
     1921 ggcagccata aggtcgaggc cgtgttcgac ggccaggccg actccggccc gtcgcgcggc
     1981 agcatgcaag tcaccgtggc cgccaccaag ctcgtgttgc agacgagcgg cgcgggcgtc
     2041 accgcccggc tcaccaccgg cgacggccag cccgtggcca aagccctgat caagttcctg
     2101 gtggacggca cggccgatag cgccaccgcc accgacgccg ccggcaacgc cgagtggcgc
     2161 acgcgccaga ccctggccgc gggcggccac aaggtcgaag cggtgttcga gggcctgccg
     2221 ggcctgctgg gcacccaggc gaccgtccaa ctcggcatcg gcggcacgaa gctgaccgta
     2281 cagatgccgt ccaacgccgg gcagggcggc acccgcatcc cggtcgtcgc ccatctcacc
     2341 acggcgggtg gccagccggt cgccaagacc gtggtcgagt tccaggtgga cggcaagcac
     2401 gacggcgagg ccctgaccga tgcggcaggt aacgccacct ggcgcctgcg cgacggcctg
     2461 gccgccggca accatcgcgt taccgccgta ttcgccgggt cgcccgcgct ggccggggcc
     2521 accgcgacca cgcaggtcgc cgtcggcgcc gcccggctgg agttccgggc cggcggccag
     2581 tcggccatca tcgcgcacct gagcgcgggc ggccggccca tcgcgcagat gcccatccac
     2641 ttcctggtcg acggcaagga ggccgggcag gtggcgacca atgcggcggg cgacgcgacc
     2701 tgggccccca aggggcaact gagcgcgggc aaccataagg tcgacgccgt gttccgcggc
     2761 gcgcccggcc tcctcccggc ccagatcacc acccagtggg ctgtgggcgc caccaccctc
     2821 accgtgcgcc cggtggccgc gaccgcggcg ggcggcacgc gccagcaggt ggaggcccac
     2881 ctcgtggatg ccgcgggcaa gcccgtggtg aaagccacga tccagttcct ggccgacggc
     2941 gcccgcggcg gcgaagccgc caccgatgcc aacggcatcg ccacctggca tatccgccgc
     3001 gacctcgcgg cgggggcgca caagatcgac gccaccttcg ccgggctgcc cggcctggtc
     3061 gcgtcgcagg cttcgaccca ggtcgaggtt ggcgcctcca agctcgccct tcagcaggac
     3121 cccgcggcgg tgggcggcac ccgcgtaccg accgtggccc acctgaccga cgccgccggc
     3181 aagccgatta gcggcgcagt gatcgaattc cagatcgacg gtgtgcgcga cggcgaagcc
     3241 gccacggacg ccaacggcaa cgccacctgg cgcgtgcgcc aggcgcccac cgagggctcg
     3301 cataaggtgg cggccatctt ccacggcatg ccgggcctgc tcggcgccga ggcgagcacg
     3361 cagttcagcg tgggcccgac caccctggcc gtgcgggtcg tcaccgacgc ctcgcagagc
     3421 ggggagcgcg ccccggtcac ggcgcacctg atcgacggcg cgggcaagcc catcgcgggc
     3481 ggcgtggtcg cgatcctggt ggacggcaag cgcgacggcg aagcggcgac cgacgccgcg
     3541 ggcgacgccg cgtggcgcat ccgcgccgat ctgacccccg gcgcgcacaa gatcgaggcc
     3601 cagttcaccg gcttgccggg cctgctgccc tcgacggctg cgacgcaact gaccgtggcc
     3661 gccacccggc tgacgctgga tcaggtgccg ctgggcgcgg cggtgggcga tcccttcgtg
     3721 atgcaagcgc ggctgagcaa cgccgacggc acgcccctga ccgacgcgcg ggtcgagttc
     3781 ctggtgaacg gcagccgtga cggcgaagcg cgtaccgacg cgacgggcac cgtcaactgg
     3841 cggctgcgcc gcctgctgag cgcgggcacc tacaccatca ccgcctcgtt ccccggcgtg
     3901 cccggcctcg aaccggagtc gcgctcgacc caggcgaccg tccaacccaa ggagatcgag
     3961 atccagaccg tgccggccgt gccgggcatg cagttccaac ttgacgacaa aaccttcacc
     4021 agcgatgcca acggcattgt ccgcatgccg gtgtcggagc cgcggaccta caagctcaag
     4081 ccgctgccgc tggacgccac gaaggccggg ctgcgcgctg agttcgtgcg ctggggcgac
     4141 gatatcttca cctctgagcg cgatctcatc gtgccggccc gcgaccgcta ccagatcggg
     4201 ttcaacgtca gctacctggt caacctcacg ttcgccgacc tcggcgggcg cacggtggac
     4261 ccggcgcgcg tgacctcgat ctcgctcaag agcagcaacg gcaaggacac gttcaccggc
     4321 caatcgactt cgcgctggct gctcggcagc ggcgtgaccc gcacggcggt cggcctgcag
     4381 gaacgcaagg tgcagtacgc cctcgaagaa gctatcgtcg gcggatccaa tgtcgtgaac
     4441 cagtccgagc agcgcttcac gccgggcacg gccacctcct ggccgatcac cctgcgcttc
     4501 tatgccgtcc acttcgtcgc ccgcgacgcg ctgttcgact tcccgatggg caagacgatc
     4561 cggctcgaat accccgacaa gcacgtgcag accgtctcgt tggggacgaa cgccgaggcc
     4621 actctcgatg ggctgccgcg cggcccctat caggtcagcg tgaccaacgt gccgggtatc
     4681 tcgcccgcca cgcccatcgc cctctcgcag gaccagcaag cgcgcttgct ggtgctcagc
     4741 tacctcgaca tcggcgtggc gctgggcgtg ctggggctga tcgcgctggg gttgctgctc
     4801 gtcgggcgct tccaggtggt cgccaaagtg cggcgccgct tcggctggga aaaactgggc
     4861 gtggccccgg cccaccggac ggtgcggctg cccgaacagg agcgcgctct cctggccgcc
     4921 acggcgccgg tcgctgtgga cgaccacacg ccggtgaata ccacgtcgcg ctacgcggtg
     4981 cccgaactca tgacctatga ggtggtcaac ggctcgcgca acgtggagcg catcctgctc
     5041 ggcgaccggg cgtgggaacg cacgcccaac ggcgagtggc gcgtcagcgc ccgcgccaag
     5101 gacgccgatc cggccgccga cggcgtgctc gacggcgtac
//
LOCUS       DASUYC010000255         2746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1461623, whole genome shotgun sequence.
ACCESSION   DASUYC010000255 DASUYC010000000
VERSION     DASUYC010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1461623"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2676
                     /locus_tag="VF276_04110"
     CDS             <1..2676
                     /locus_tag="VF276_04110"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX6606078.1"
                     /translation="RYTQHPLPISKGVIGRAVREDRTLLIRDVHHDPDYITVTPDIRS
                     QLVVLVRRGGQVLGALSLESQDPDGFHTEHVEFTEHLAEHSAIAVENARRYERERRQN
                     EALTRRAGELAAILRIGNVLKADLSLDEVLTQVAEGVRSSLGFNVAILSLIDEGPPRR
                     FVRVAAAGLDAETWARLKTAMPPAEESNKLLDPRFQISESYFISHLYNPVTHLETYFR
                     PDITPRDETEWHPDDMLLVPLRGKGGQLVGVLSVDDPVDRQLPSRATIETLEIFANQA
                     AVAIENARLFEAQRRRLRELSLLQESGVGLNATLDPTELLAAVVRSAVQLFEVRASAI
                     TLVPDPSNPRMSTASLFCEARDGVLEVRPYSAVDQPELTQEIILGGAPVVVPDATADP
                     RLSPDLVARGLRSLAGVPLRVGNQAQGVLYLFGDRAQPLDSQEQQLLRIFANQAAVAV
                     QNARLFEEETRRLQDVTSLQDLGLRFTATLDIRTLLEEVVRAANHLLDTNSSGVMLVD
                     ETGTQAVNVRCWVREGGQVETRSFNRAIRRDGLTNTVMRTGRPIMLAETLGDPRINPA
                     MMAEGIRSFVMVPIRIAEQTLGTLFVNSYQPRAFSAHEQQLLQVFANQVAVAIKNALL
                     FEERRDFEQRLIGENERMARELVTARATQRQLLPELPRRVAGLTMHGICLPAMEVGGD
                     YYDLVPLPDGRLALALGDVTGKGTAAVMLMAMVKTALLSQVSTDPQPHPVLQTLNTLA
                     VDLMQGQLMTFFYALYDPATRVLTYSNAGHLYPYVRRADGTLDYIAGGGLPLGALPTV
                     APKVFTLHVAPGDLLVFFSDGIVEAADSGGEMFSFERVEETLRQVPPGADLRLLVEDI
                     VDRVHAFAGGAPQDDVTLLIARVDDGESAAGTPGS"
BASE COUNT          430 a          959 c          926 g          431 t
ORIGIN      
        1 cgctacaccc agcacccgct gccgatcagc aagggagtga tagggcgcgc cgtgcgcgag
       61 gaccgcacgc tgctcatccg cgacgtgcac cacgatcccg actatatcac cgtcacgccc
      121 gatattcggt cgcaactggt ggtgctggtg cgccggggcg ggcaggtgct gggggccttg
      181 agcctggaga gccaagaccc ggatggcttc cacacggagc acgtcgagtt taccgagcac
      241 ctggcggagc actccgccat cgccgtggag aatgcccgcc gctacgagcg cgagcgccgc
      301 cagaatgagg cgctgacccg ccgggccggg gaactggccg ccatcttgcg catcggcaac
      361 gtgctgaaag ccgatctctc gctggacgag gtgctgaccc aggtggccga aggtgtgcgc
      421 agcagcctgg gcttcaacgt cgccatcctc agcctcatcg acgaggggcc gccgcgccgc
      481 tttgtccgcg tggccgccgc gggcctggat gccgaaacct gggcgcggct gaagacggcc
      541 atgccgcccg ccgaggaaag caacaaattg ctcgacccgc gcttccagat cagcgagtcg
      601 tatttcatca gccacctgta taacccggtg acgcacctgg agacctactt ccggcccgac
      661 atcaccccgc gcgacgagac cgagtggcac cccgacgaca tgctgctcgt gcccctgcgc
      721 ggcaaaggcg gccagttggt cggcgtgctc tcggtggacg acccggtgga ccgccagttg
      781 ccctcgcggg cgaccattga aacgctggag atcttcgcca accaggccgc ggtggcgatt
      841 gagaacgcgc ggctgttcga ggcccagcgc cgccgcctgc gcgaactttc gctgctgcaa
      901 gaatcaggcg tcggcctcaa cgccacgctc gatccgacgg aactgctggc cgcggtggtg
      961 cgctcggcgg tgcaactgtt tgaagtccgc gccagcgcga ttacgctggt gcccgacccg
     1021 tcgaacccgc gcatgtccac cgcgtcgttg ttctgcgaag cccgcgacgg cgtgctggag
     1081 gtgcggccct atagcgcggt ggaccagccg gagctcaccc aggagatcat cctcgggggc
     1141 gccccggtcg tcgtgcccga cgccaccgcc gatccgcgcc tcagtcccga cctggtggcg
     1201 cgcgggctgc gctcgctggc gggcgtgccg ttgcgcgtgg gcaaccaggc ccagggcgtg
     1261 ctctatctct tcggcgaccg cgcccagccg ctcgacagcc aggagcaaca gttgttgcgc
     1321 attttcgcca accaggccgc ggtggcggtc cagaacgccc gcctgtttga ggaagaaacc
     1381 cgccgcctgc aggatgtcac atctctgcaa gacctgggct tgcgctttac cgccacgctc
     1441 gatatccgca cgctgctcga agaagtggtg cgcgcggcca atcacctgct cgacaccaac
     1501 tccagcggcg tgatgctcgt cgacgagacg ggcacgcagg ccgtcaacgt gcgctgctgg
     1561 gtgcgcgagg gcggccaggt ggaaacccgc tcgttcaacc gcgccatccg gcgcgacggc
     1621 ctgaccaata cggtcatgcg caccgggcgc ccgatcatgc tggccgagac gctgggcgat
     1681 ccgcgcatca atccggcgat gatggccgag ggcatccgct cgttcgtgat ggtgccgatt
     1741 cgcatcgccg agcagacgct gggcacgctg ttcgtcaaca gctatcagcc gcgcgctttc
     1801 tcggcccacg agcagcagtt attacaggtg ttcgcgaacc aggtggccgt ggcgatcaag
     1861 aacgcgctcc tgttcgagga gcgccgcgac ttcgagcagc ggctgatcgg cgagaacgag
     1921 cgcatggccc gcgaactggt gacggcgcgc gccacccagc ggcagttgct gccggaactg
     1981 ccgcgccgcg tggccgggct gaccatgcat ggcatctgcc tcccggcgat ggaggtcggc
     2041 ggcgactatt acgacctggt gccgctgccc gacgggcgcc tggccctggc gctcggcgac
     2101 gtgaccggca agggcaccgc cgccgtcatg ctcatggcga tggtcaagac ggcgctgctg
     2161 tcgcaggtga gcaccgatcc gcagccgcac ccggtgttgc agacgctgaa caccctggcg
     2221 gtggacctga tgcagggcca actgatgacc ttcttctacg cgctctatga cccggccacc
     2281 cgcgtgctga cctacagcaa cgccgggcac ctctacccct atgtccgccg ggccgacggc
     2341 acgctggact atatcgcggg cggcggcctg ccgctcggcg ccctgcccac cgtcgcgccc
     2401 aaggtgttca ccctgcacgt cgcgccgggc gacctgctcg tctttttctc cgacggcatt
     2461 gtggaggcgg cggacagcgg cggggagatg ttcagcttcg agcgggtgga ggagaccctg
     2521 cgccaggtgc cgcccggcgc cgatttgcgc ctgctggtcg aggatattgt ggaccgggtc
     2581 catgccttcg ccgggggcgc gccgcaagac gatgtcacgc tgctgatcgc ccgcgtggac
     2641 gacggcgaat ctgctgccgg gacgccgggt tcgtaacgcg cccggcgcat cgggcctggt
     2701 ggggccgctt cccgaccacc ttgcgcgggc cggtttgaaa ccggcc
//
LOCUS       DASUYC010000256         2146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1903686, whole genome shotgun sequence.
ACCESSION   DASUYC010000256 DASUYC010000000
VERSION     DASUYC010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1903686"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /locus_tag="VF276_04115"
     CDS             <1..1072
                     /locus_tag="VF276_04115"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX6606079.1"
                     /translation="LPSATPVAAPTARRTGNTTPGLTWQLVGLTGQELFAVGGSGKAG
                     SPVYAGGTGLYRSDDDGATWTQLSLGNSTTVEEIHASASKPQTLFAGSGANCYGGPPG
                     GQFRSDDGGLNWTPLKEAPASIQIDSQNPERLTAMSCQGVVRSTDGGQTWAPLTDAVQ
                     ARLKNFVGDEVRVPASNGSMIYAVYVSEGGTSRIRVSTDDGKTWSGPDADYAGVSDIL
                     VDDQKPSRAWALSQNGVLLTTDSGQTWTPDVTGLDAAHNTAAGSQGAYQLSSLAAQYS
                     NAGALVELYTGSFGTESQPAAGVFSSKDFGTVWTRFGGDLGGAAIHQLFVTREQGSSG
                     EQVVLYAATDDGLHKITLGSAR"
     gene            1426..>2146
                     /locus_tag="VF276_04120"
     CDS             1426..>2146
                     /locus_tag="VF276_04120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX6606080.1"
                     /translation="MPSLALVIVNYNTRELLTGCLDSVFASRCRYPFHVTLVDNNSTD
                     GSVELVRTQYPQVTLLESAHNGGFGYANNIALRALAVPPRPASPQQPASEIPEQGDAG
                     IRFSTDYILFLNPDTVLPPDALEVMVDFLERTPQAGVVGPRMEKPDGTLDLACRRAFP
                     TPLNSLFKLTGLSRLFPRHPLIARYNLTHLREDELAEVDSVMGAFMLVRGTALGQAGL
                     FDERFFMYGEDLDLAYRIKARG"
BASE COUNT          368 a          738 c          681 g          359 t
ORIGIN      
        1 gctccccagc gccaccccgg tcgccgcgcc gaccgcccgg cgcaccggca acaccacgcc
       61 gggcctcacg tggcaactgg tggggctcac cggccaggag ttgttcgccg tgggcggcag
      121 cggcaaggcg ggcagcccgg tctacgcggg cggcaccggc ctgtatcgct cggatgatga
      181 cggcgccacc tggacgcaac tcagcctggg caattcgacc acggtggagg aaatccacgc
      241 cagcgcgagc aagccgcaaa ccctcttcgc gggcagcggt gccaactgct acggcgggcc
      301 gcccggcggc cagttccgca gcgacgacgg cggcctcaac tggaccccgc tcaaagaggc
      361 gcccgcgtcc atccagatcg actcgcagaa tcccgaacgc ctgacggcca tgtcttgcca
      421 aggcgtggtg cgcagcaccg acggcgggca gacctgggcg ccgctcaccg atgccgtgca
      481 ggcgcggctc aagaatttcg tcggcgatga ggtgcgcgtg cccgcatcaa acggcagcat
      541 gatctacgcc gtctacgtga gcgaaggcgg cacgagccgc atccgcgtca gcaccgacga
      601 cggcaaaacc tggtccggcc ccgacgccga ctatgccggc gtgagcgaca ttctggtcga
      661 tgaccagaaa ccgtcgcgcg cctgggcgct ctcgcagaac ggcgtgctgc tgaccaccga
      721 cagcggccag acctggacgc ccgatgtgac cgggttggat gccgcgcaca acacggccgc
      781 cgggtcgcag ggtgcctacc agctttcgag cctggcggct cagtacagca acgccggcgc
      841 cctggtcgaa ctctacaccg gaagcttcgg caccgagagc cagcccgccg cgggggtctt
      901 cagcagcaaa gacttcggca cggtctggac ccgcttcggc ggcgacctgg gcggggccgc
      961 catccaccag ttgttcgtca cccgcgaaca gggctccagt ggtgagcagg tggtgctcta
     1021 tgccgccaca gacgacggct tgcataagat cacgctcggt agcgcgcgct gacggcgcat
     1081 ccggtcagcg cggattcatg cgggggtggc cgccgtgcca cccctgcccc gtgtccggcc
     1141 cggtgaaagt ccccccgcag tctccgccga gaggctgagg aactttccga ctaccgttgg
     1201 tagaatgaaa gggtcggatt gtgcgacctg cggcatgccg gctcgtttta ttattgacgg
     1261 aattgagacc tgggcagcgg cccccccttc gcgggcgcct tggccccgat cttgccggac
     1321 ttgaggcgaa agtgcggctg ttctgccaga tagagggatc ttctggtggt tacaggccgc
     1381 tattgaccca tcacccatca ttgcgaattc tggtataatc gccctatgcc tagcctggct
     1441 ctcgtgattg tcaattacaa tacgcgcgaa ctactgacgg gctgtttaga ttcggtgttt
     1501 gccagccgtt gccgttatcc gttccacgtt accctggttg ataacaacag caccgacggc
     1561 agcgtagagc tggttcgtac ccaatatccg caggtgacgc tgctggagag cgcgcacaac
     1621 ggcggcttcg gctatgccaa caatatcgcg ctgcgcgcgc ttgccgtgcc gccgcgcccg
     1681 gcgtcgcccc agcaacccgc gagcgagatc cccgagcagg gcgatgcggg catccgattc
     1741 agtaccgact acattctctt cctcaacccg gacacggtgc tgccgcccga cgcgctcgaa
     1801 gtcatggtcg attttctgga gcgcacgccg caggccggcg tcgtgggccc gcgcatggag
     1861 aagcctgacg gcacgctgga cctggcgtgc cgacgtgcct tccccacgcc gctcaactcg
     1921 ttgttcaagc tcaccggcct ctcccgtcta ttcccgcgcc atccgctcat cgcgcgctac
     1981 aacctgaccc acctgcgcga ggatgaactc gccgaggtcg attcggtaat gggggccttc
     2041 atgctggtgc gcggcacggc gctgggccag gccgggctgt ttgacgagcg tttcttcatg
     2101 tatggcgaag acctggacct ggcctaccgc atcaaagcac gtggct
//
LOCUS       DASUYC010000257         2212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_776922,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000257 DASUYC010000000
VERSION     DASUYC010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776922"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="VF276_04125"
     CDS             <1..1016
                     /locus_tag="VF276_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6606081.1"
                     /translation="GRVSAGTWFLFVQSIGVFWFPLTGIASFWNQFQQGLAAGERIFA
                     LIDAQPRVQQTDRRPVPRVDGRIEFHDLDFRYTDKESVLEDFNLTIPAGQTIALVGHT
                     GAGKSTLGKLVARFYEFQGGELLIDGRDIRTFDLQMYRRHLGVVPQVPFLFAGTVADN
                     IRYACPGASDAAVEEVARQIGGGEWLAALPDGLATDVGEGGRALSLGQRQLVALARVL
                     LQDPSIVILDEATASVDPLTEAQIQEGLDLILQHRTAIIIAHRLSTIRHADRIIVLRQ
                     GRIMEEGSHDALMARGGHYAQLYNTYFRHQSPDYRPGEGFVPIRLPLSDLEPEPEEQA
                     LAG"
     gene            complement(1035..1499)
                     /locus_tag="VF276_04130"
     CDS             complement(1035..1499)
                     /locus_tag="VF276_04130"
                     /inference="COORDINATES: protein motif:HMM:NF015678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HEX6606082.1"
                     /translation="MSNYEQARVAGQASEYAQAYPITPAQTQVLPPMPQPGTAYPTPA
                     PPYTPVAQPAYTAPVYQQQPVYAPPPPVYMPQIVQQVNVVQQTTSTAAPVYYVNPGPS
                     MLVCVIYFLFVGWWVGLTWLFIALGLMCTIIGIPIGVIMLRYLPQVTFLRSL"
     gene            complement(1817..>2212)
                     /locus_tag="VF276_04135"
     CDS             complement(1817..>2212)
                     /locus_tag="VF276_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013224375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606083.1"
                     /translation="EHLERDPWLLALLRGRSRAALMDALAEARARLVPDEPASPVPEP
                     ELLPPPEPVLPPPPPAPPANFWEAGAGLADFYVEIAAPPQPAPLLQQLGPPSFWTPPA
                     AFAAQLEPAYAAVTAAVLRLVYGDDAEIA"
BASE COUNT          410 a          718 c          741 g          343 t
ORIGIN      
        1 acgggcgcgt gtcggcgggc acctggttcc tgttcgtgca gagcatcggc gtcttctggt
       61 tcccgctgac cggcatcgcc tcgttctgga accagttcca gcagggcctg gccgcgggcg
      121 aacgcatctt cgccctgatc gacgcccagc cgcgcgtgca gcagaccgac cggcggcccg
      181 tgccgcgcgt cgatggccgc atcgagttcc acgacctcga cttccgctac accgacaaag
      241 agtcggtgct ggaagacttc aacctgacca tccccgccgg gcagaccatc gccctggtcg
      301 ggcacactgg cgcgggtaag tccacgctgg gcaagctggt ggcccgcttc tacgagttcc
      361 agggcggcga actgctcatc gacggacgcg acatccgcac cttcgatctg caaatgtacc
      421 gccgccacct gggcgtggtg ccgcaggtgc ccttcctctt cgcaggcacc gtggccgaca
      481 acatccgcta cgcctgcccc ggcgcgtccg atgccgccgt ggaggaggta gcgcggcaga
      541 tcggcggcgg cgaatggctg gcggcgctgc ccgacggcct ggcgaccgac gtgggcgagg
      601 gcgggcgtgc tctgtcgctc ggccagcgcc agttggtcgc gctggcccgc gtgctgttgc
      661 aagacccgtc catcgtcatc ctcgacgagg ccacggccag cgtggacccg ttgaccgagg
      721 cgcagatcca ggaggggctg gacctgatcc tgcaacaccg cacggcgatc atcatcgccc
      781 accggctctc gaccatccgc cacgccgacc gcatcatcgt gctgcgccag ggccgcataa
      841 tggaggaagg cagccacgat gcgctaatgg cgcgcggcgg ccactacgcc cagttgtaca
      901 acacctactt ccgccaccag tcgcccgact accgccccgg cgagggcttc gtgcccatcc
      961 gcctgcccct gtccgacctg gaaccggaac cggaggagca ggcactggca ggctgagacg
     1021 gcgggaaccg aaccctatag cgaacgaagg aaagtcacct gaggcaaata gcgcagcatg
     1081 atgacgccga taggaatccc gataatagtg cacatcaacc cgagagctat gaacagccac
     1141 gtaagtccta cccaccagcc cacgaacaag aagtaaatca cgcacacaag catgctcgga
     1201 cctggattca cataataaac cggggcggca gtgcttgttg tttgttgaac cacgttcacc
     1261 tgttgcacga tttgcggcat gtagaccggc ggcggcggag catacaccgg ctgttgttga
     1321 tagaccggag cggtataggc gggctgagcc acgggcgtat agggcggggc gggtgtggga
     1381 taagcagtgc caggttgcgg catcggtgga agaacttgcg tttgcgccgg ggtgatggga
     1441 tacgcctggg cgtactcgct tgcctgcccg gccacccgcg cctgttcata gttgctcaag
     1501 gacgaggggt ctaagggtaa ggcgcaaccg ggacagaaag cctgacccgc ctggatgggg
     1561 ccgctacaac ggggacaatg gtaggacgtg atcattgctt actcctgggt acacgatgaa
     1621 gaaagagtgg gacagccggc ttatgctcgc gcagaggtgg ctattccgac ggtatgcacc
     1681 ggcgcgcggt tcaggtcaat tttagcgcgg aagcaaccgc tggttgtagg gcgcattgtc
     1741 cctcaaacgc ttgcccgatc cgccccatct ccgcagaggg gataaccaat agcatgagga
     1801 ttgccaggag gccgattcag gctatctccg catcatcacc atacaccagc cgcagcacag
     1861 cggcggtcac ggcggcgtaa gccggttcca gttgcgcggc gaaggcggcg ggcggcgtcc
     1921 agaaggacgg cgggccgagt tgttgcaaca gcggcgcggg ctgcggcggc gcggcgatct
     1981 cgacgtagaa atcggccagc cccgcgcccg cttcccagaa gttcgccggg ggcgcgggcg
     2041 gcgggggcgg cagaacaggt tcgggcggcg gaagcaattc cggttccggc acgggggaag
     2101 ccggttcgtc cggcaccagc cgggcgcgcg cttcggccag ggcgtccatc agcgcagcgc
     2161 gcgaccggcc ccgcaacagc gccagcagcc aggggtcgcg ctccaggtgc tc
//
LOCUS       DASUYC010000258         2546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_948230,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000258 DASUYC010000000
VERSION     DASUYC010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948230"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1219
                     /locus_tag="VF276_04140"
     CDS             <1..1219
                     /locus_tag="VF276_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606084.1"
                     /translation="TNTAVVPTNTPTNTPTNTAIVPTSTPTNTPTNTAVVPTRTPTNT
                     PTNTAVAPTRTPTNTPTNTAVAPTRTPTNTPTNTAVPPSRTPTNTPTNTAVAPSRTPT
                     NTPVPPSRTPTRTATATRTPVTCSVCNVYIADVNISCNPDGTVHWDALVRNNGACTVF
                     SPWRTDLQQQRNYSDTFTTVDTQTGNGVFPPGDTHVSGDFPPHTFPADTTFIRVQFQL
                     TSDEVECNPDPYSPLIEPCLVGGPTQTPRATATRTPARTATVTRTPGPATATRTPARP
                     ATATRTPVTCSVCNVYVPDVNIACNPDGTVHWTAMIRNNASCTVSSPWKVELQVQKNY
                     GRFTTVLTQTGSGVFPPDDTLIGGDFCYTAPANATNLRVRFTLTSSERACNPDPISQM
                     IEPCVPQDPCPVP"
     gene            1421..1927
                     /locus_tag="VF276_04145"
     CDS             1421..1927
                     /locus_tag="VF276_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606085.1"
                     /translation="MSTVSAEALINSVVILLTEAYAGPPDPSTTWFIDNEANSGILGA
                     LEGVSAAEASRSVDGSGQEGSTIAANAEHLRWSLAQFNAAIRGGAYEGRWEESWQVTS
                     VGEDDWDNLRRALRTEFETLCALLKPQESLPEDYITGTLASVAHAAFHLGLIRQMIAR
                     VRQAAEPG"
     gene            complement(2005..>2546)
                     /locus_tag="VF276_04150"
     CDS             complement(2005..>2546)
                     /locus_tag="VF276_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606086.1"
                     /translation="LDQMRAWPNAPQSVAAMVLYDATGGRLAAIAQTLVGQSLGPPDP
                     AATFVSVCGDFWYYILRTVSRVQRGQAWGARQDYHSLVLNLLSALLRLEVGATARWRA
                     SEAAVGIEAVLSPARLAQLEGCIPGPGLAGVEAALARAAELGWMTCRAGAARYARSWP
                     EQLARDILHLLGTAPPDGG"
BASE COUNT          467 a          939 c          805 g          335 t
ORIGIN      
        1 aacgaatacg gcggttgtgc cgacgaacac accgaccaac acgcctacta ataccgctat
       61 cgtgccgacg agtacgccga ccaacacgcc gacgaatacc gccgtggtac cgacgcgcac
      121 gccgacgaat acaccgacta ataccgccgt tgcaccgacc cgtacgccga cgaacacgcc
      181 gaccaatacc gccgtggcac cgacaaggac cccgacgaat acgccgacga atacggctgt
      241 gccgcctagc cgcacgccga ccaacacccc gacaaatacc gccgtggcgc cgagccgcac
      301 gccaaccaac acgccggtgc cgccgagccg cacgcccacg cgcacggcca cggccacgcg
      361 cacgccggtg acgtgcagcg tctgtaacgt ctacatcgcc gacgtgaaca tctcgtgtaa
      421 ccccgacggc accgtccact gggacgcgct ggtgcgcaac aacggcgcgt gcacggtctt
      481 ctcgccgtgg cgcaccgacc tgcagcagca acgcaactac agcgacacct tcaccacggt
      541 cgatacgcag accggcaatg gcgtcttccc gccgggcgac acccacgtca gcggcgactt
      601 cccgccgcac accttcccgg ccgacacgac cttcattcgc gtgcagttcc aactgaccag
      661 cgacgaggtc gagtgcaacc ccgatcccta ctcgccgctg atcgagccgt gcctggtggg
      721 cggcccgacc cagacgccgc gggctaccgc cacccgcacc cccgcgcgca cggccacggt
      781 cacgcgcacg ccgggcccgg ccacggccac gcgcaccccg gcgcggccgg ccacggccac
      841 ccgcacgccg gtgacgtgca gcgtctgcaa cgtctacgtg cccgacgtga acatcgcctg
      901 caatcctgac ggcacggtcc actggacggc gatgattcgc aacaacgcca gttgcacggt
      961 aagctcgccc tggaaggtcg agttgcaggt gcagaagaac tacggccgct tcaccacggt
     1021 gctgacccag accggcagcg gcgtcttccc gcccgatgac accctcatcg gcggcgactt
     1081 ctgctacacg gccccggcca acgccaccaa cctgcgcgtc cgcttcacgc tgaccagcag
     1141 cgagcgggcc tgtaaccccg accccatctc gcagatgatc gagccctgcg tgccgcaaga
     1201 cccctgcccg gtgccctgac gagggcgggc gggctatgac tataggcaga ggggatccgg
     1261 ttagccgggt ccccttcgcg cgccccgcta acgataccgc cggcgaactt ccacttaagg
     1321 gctagctggc gtagagatcg gtgcatcgat ctgcctgttg gcgctgcgaa agcagatgtg
     1381 cgtgggcacg ccggcgcatt tcacaaggag gacccacaca atgagcacag tctcagccga
     1441 ggcgttgatc aactccgtag tcatcctgct taccgaagcc tacgccgggc cgccggaccc
     1501 gtcgaccacc tggttcatcg acaacgaggc gaactccggc atcctcggtg cgctggaagg
     1561 cgtgtctgct gcggaggcgt ccaggtcggt cgacggcagc gggcaggagg gctcgaccat
     1621 cgccgcgaac gccgagcatc tacgctggag cctggcgcaa ttcaatgccg ctatccgtgg
     1681 cggggcctac gagggcaggt gggaggaaag ctggcaggta accagcgtcg gtgaagacga
     1741 ctgggataat ctgcggcgcg cattacgcac tgagttcgaa acgctgtgcg cgctgctgaa
     1801 gccgcaagaa tccctgccgg aagattacat aaccggcacg ctggcctcgg tcgcgcacgc
     1861 tgccttccat ctgggcctga tccgccagat gatcgcccgc gtacggcagg cggccgaacc
     1921 gggctgacgc gggcaccggc agggcagatt tgaggccgcg caaggcggtc tttgtagggg
     1981 tagccggcgg cttgagcctc cgggctaccc gccgtccggc ggcgcggtgc cgagcaggtg
     2041 caggatgtcg cgcgctaatt gctcgggcca ggagcgagca tagcgggcgg cgccggcccg
     2101 gcaggtcatc cagccgagtt cggcggcgcg ggccagggcg gcctccaccc ctgccaggcc
     2161 ggggccgggg atgcagccct ccaactgcgc cagccgcgcc ggcgacagca cggcctcgat
     2221 gcccaccgcc gcctcggagg cccgccagcg cgccgtcgcg cccacctcca ggcgtagcag
     2281 cgcgctcaac aggttgagca ccaggctgtg gtagtcctgg cgcgcgcccc aggcttgccc
     2341 acgctgcacc cggctcaccg tgcgaaggat gtaataccag aaatcgccac agacgctgac
     2401 gaacgtggcg gcggggtcgg gtgggcccag cgattgcccg accagcgttt gcgcgatggc
     2461 cgccagccgc ccgccggtcg catcgtagag caccatcgcg gccaccgact gcggggcgtt
     2521 gggccaggcc cgcatctggt ccaggg
//
LOCUS       DASUYC010000259         5677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1324141, whole genome shotgun sequence.
ACCESSION   DASUYC010000259 DASUYC010000000
VERSION     DASUYC010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5677
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324141"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VF276_04155"
     CDS             <1..516
                     /locus_tag="VF276_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HEX6606087.1"
                     /translation="AEAAAQVAATRTDLAAAPDIYWTGYVQDAQKEFAEASIIHAVVA
                     GQTVPGAADLGVEAAPYLNGLGEAAGEMRRYVLDIIRHGQLARGEEVLGVMEEIYSLL
                     VTIDYPDAVTNGLRRTTDMVRGVLERTRGDLTFAIQQQILTQALAQHLPAGTTVDLPG
                     YMPAGDDGEGA"
     gene            522..872
                     /locus_tag="VF276_04160"
     CDS             522..872
                     /locus_tag="VF276_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606088.1"
                     /translation="MPPRRRAVRPVSGPPAPKEAPAAATPPNRPRSTGYIVRASELGS
                     YSYCRRAWWLRYVAKLEPPEAARARLEGGHLRHAEHGRGLWLAGVLWKLGLVCLMLAG
                     ALALLWLLTSVLVH"
     gene            899..1765
                     /locus_tag="VF276_04165"
     CDS             899..1765
                     /locus_tag="VF276_04165"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEX6606089.1"
                     /translation="MARIGFVTDSTAHVPEELIAKYDLRIADLTVQFGQESFREHADL
                     DTDDFFRRLKESHTLPTSSQPSPEEFLRAYKSLAQDHDSIICVTLSHKLSGTINSARL
                     AAQMVESDGVKVTVIDSLSAWMGTGLQVIAGAQAAAAGKSHDECVALIEELVKRMQVL
                     LVVDTLEYLQRGGRIGGAQAMIGTMLQIKPVLTVRDGMLQPLERVRTKRKALDRILDI
                     MAEHVGTQPYTAALGHAQAEAEAEELRKRILARMPQVRDMYLSPVSPVVSVHLGPGAI
                     GLVYYVNLPDQP"
     gene            1774..2232
                     /locus_tag="VF276_04170"
     CDS             1774..2232
                     /locus_tag="VF276_04170"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6606090.1"
                     /translation="MQRIAHFILIDAPIERVFDFVADYHNVPRMQTHFKSVRLLSAQE
                     RGQGAQIEALGSFRGMPLTAQMTIIGFDEPHVLVSDSTGGVRSHTTWRFDAHPGATPD
                     TPPRTRAAVVIEYEITIPGLSLLSGLVHRDVDNMTMDSLRRLKLLVEAES"
     gene            2317..2718
                     /locus_tag="VF276_04175"
     CDS             2317..2718
                     /locus_tag="VF276_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX6606091.1"
                     /translation="MGGICVSRVERFEDRIAWQKARALPAAVYEVTRQSSFARDVGLS
                     SQIQRAAVSIMPNIAEGFERNNPAEFHQFLSTAKASCAELRSQLYVALDAGYLSQERF
                     TQLLQQAEEVGRIIGGLRASVERQRNEQRGR"
     gene            complement(3019..3441)
                     /locus_tag="VF276_04180"
     CDS             complement(3019..3441)
                     /locus_tag="VF276_04180"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX6606092.1"
                     /translation="MAQQLANLRASRSQFDPDSEQVRIYFDAAEDGYATLVVFNGQGT
                     WVLSLGEIQIRAGANTLVWHGHDADGWPLPVGPYRFELFGFGCDRRPTGAGPLHLPIT
                     LVRRPAEVDDDPDLPWRRRAWSPASREAADGWSSAPLR"
     gene            complement(3598..>5677)
                     /locus_tag="VF276_04185"
     CDS             complement(3598..>5677)
                     /locus_tag="VF276_04185"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6606093.1"
                     /translation="AWHTNSADYTALPTPASVRRVLEQRLARLTPAARSVLEVGAVLG
                     REVPETLLWRASGSTPEAVLGPAEELLRHQLWVEDEAGRGYAFAHQKVREVAYAGISG
                     PRRRHLHRQAAGALEAEHPQAVDRLADHWLAADDPLRALPYLQEAAERAVAAFANDAA
                     LAFYDQALAAVERLPAGIGEEDIERVYALVAGRAAINKRLERMEAATADLDRLVALAR
                     TGNQPGRVADALARRSAHFISQARYAEAQADLAAALALAEAGHNSAAVARILTQVTRV
                     QIRLGDHRAAIAAAERALVRYRDLGDLRGEVEILSLLGPVYGEIGELDRAVALLEEGL
                     AKVRQVPQAVHLEARLLNNLALYVGAPATSYDLLTRFLALADETGSLPLQETAHQNLA
                     ELLARFGRYAEAQAHLDQALALARTAGSRHDIALLLGTRGQIQAVLGDPGTARADLEE
                     AVRMLEGLGARFSTMQALLWLGDHLLDQGSFSAGLTVVERSHALWQRLGRPAGGQYAP
                     VFPAQMARAHLGLRRAGAARHAAQDALAALDAGPPPSRLSWAEPSVEVLAHCYTVLAA
                     SSPTAEAGAVLERGYAHMMATADQCGAFLRPSYLEGVRECRVLRAAWEARVAAPDGLP
                     GGPDDDSMDAPEVERKVAGRRAELLIMLHDAAERGLALDDGALARSFGVSLRTIQRDL
                     AALRRTGRLA"
BASE COUNT          948 a         1962 c         1944 g          823 t
ORIGIN      
        1 gccgaggccg ccgcccaggt ggccgccacc cgcacggacc tggccgccgc gcccgacatc
       61 tactggaccg gctacgtgca ggacgcgcag aaggaattcg ccgaggccag catcatccac
      121 gccgtggtgg ctgggcagac tgtgccgggg gcggcggacc tgggcgtcga ggccgcgcct
      181 tacctgaacg ggttgggcga ggcggcgggc gagatgcgcc gctacgtgct ggacatcatc
      241 cgccacggcc aactggcgcg cggcgaagag gtgctggggg tcatggagga gatctatagc
      301 ctgctggtga ccatcgacta tcccgacgcc gtgacgaacg ggctgcgccg caccaccgac
      361 atggtgcgcg gcgtgctcga acggacgcgg ggcgacctca cctttgccat ccagcagcag
      421 atcttgaccc aggccctggc ccagcatctc cccgccggga ccactgtcga tctgccgggc
      481 tacatgcccg ccggcgacga cggcgaggga gcgtagattc gatgccaccg cgccggcgcg
      541 cggtccggcc ggtcagcggc ccgcctgccc ctaaggaggc accggctgcg gccactccac
      601 ccaatcgccc ccgatccacc gggtacatcg tgcgggcgag cgaactcggt agttatagtt
      661 attgccggcg cgcctggtgg ctgcgctatg tcgccaagct ggagccgccg gaagcggcgc
      721 gggcgcgcct cgaaggcggc catttgcggc acgccgaaca cgggcgcggc ctgtggctgg
      781 ccggggtgct gtggaaactg ggcctggtct gcttgatgct ggccggcgcc ctggccctgc
      841 tctggctgct caccagcgtg ctcgtgcatt agcccgcaat ctaggaggtt ccccgccaat
      901 ggcccgtatt ggctttgtca cggacagcac cgcccacgta cccgaggagt tgatcgccaa
      961 atacgatctc cggatcgccg acctgaccgt gcagttcggc caggaaagct tccgcgaaca
     1021 cgccgacctc gacaccgacg atttcttccg gcggctcaaa gagtcgcaca ctctgccgac
     1081 gagttcgcag cccagcccgg aagagtttct gcgcgcctac aagagcctgg cccaggatca
     1141 cgacagcatt atctgtgtca ccctgtcgca caagctgagc ggcacgatca attcggcgcg
     1201 tctggccgcc cagatggtcg agagcgacgg ggtgaaagtg acggtgatcg acagcctgtc
     1261 ggcctggatg ggcaccggct tgcaggtcat cgccggggcg caggccgccg ccgcggggaa
     1321 gtcgcatgac gaatgcgtgg cgttgatcga ggaactggtc aagcggatgc aggtgctgct
     1381 ggtggtcgat acgctggagt acttgcagcg cggcgggcgg atcggcggcg cacaggcgat
     1441 gatcggcact atgctgcaaa tcaagcccgt gctcaccgtc cgcgacggca tgctgcaacc
     1501 gctggagcgc gtgcgcacca agcgcaaggc gctcgaccgc atcctggaca tcatggccga
     1561 gcatgtgggc acgcagccgt ataccgcggc gctgggccac gcgcaggccg aggccgaagc
     1621 cgaggaattg cgcaaacgca tcctggcgcg catgccgcag gtgcgcgaca tgtacctcag
     1681 cccggtcagc ccggtcgtgt cggtccactt aggccccggc gcgatcggcc tggtctatta
     1741 cgtcaacctg cccgaccagc cgtaggtggc ggcatgcagc gtatcgccca ctttatcctg
     1801 atcgacgcgc ccatcgaacg ggtctttgac ttcgtggccg actatcacaa cgtgccgcgg
     1861 atgcagaccc acttcaagag cgtccggctg ctcagcgcgc aggagcgcgg gcagggcgcg
     1921 cagatcgaag cgctcggctc tttccgcggc atgccgctca ccgcacagat gactatcatc
     1981 gggtttgacg aaccccacgt gctggtgagc gacagcaccg gcggcgtccg cagccacacc
     2041 acctggcgct tcgacgccca ccccggcgcc acccccgata cccccccgcg cacccgcgct
     2101 gccgtcgtca tcgaatacga aatcaccatc cccggcctct ccctcctcag cggcctggtc
     2161 caccgcgacg tggacaacat gaccatggac tccctccgcc ggctcaagtt gctcgtggaa
     2221 gccgaaagct aacgagtgct gagtgctgag tagagacggg agttcttgag tgctggacgc
     2281 aaacgaggga tagagagtaa atgtcataca cggcgaatgg gcgggatttg cgtaagccgc
     2341 gttgagcgct tcgaagatcg aatcgcctgg cagaaggccc gcgcacttcc agcagctgtc
     2401 tacgaggtga cgcgccagag cagcttcgcc cgcgacgtcg ggctttcgag ccagatacag
     2461 cgggcggccg tctcgatcat gcccaacatc gcggaaggct ttgaacgcaa caaccccgcc
     2521 gaattccacc aattcctctc gaccgccaaa gcctcgtgcg ccgaactccg ttcgcaactc
     2581 tacgtcgcgc tcgacgccgg ctatctctcg caggagcgat tcacccaact cttacagcaa
     2641 gccgaagaag taggcagaat tattggtggc ttacgcgcat cagtagagcg ccaaagaaac
     2701 gaacaacggg gaagataact acaagaggaa cttactgctc actaaactcg taccactctg
     2761 tactcaatca ctccaactac accgactcaa ctccgtactc aacactcgtc gtccctactc
     2821 agcactcagc actcagcact cagcactaag aaagggccgg agggcggcca ccatgacggg
     2881 gcgggtggcc gccgaccgga atcagccggg acgggcgcca cgaaccaggc ggcgaaccac
     2941 cggggccgtg gattggggga aacgcctgtc ccgcctgatc ttgacctcca aagcaagagc
     3001 cggcgcgcgg gccggccgtt agcgcagcgg cgcgctggac cagccgtccg ccgcctcgcg
     3061 cgaggcgggc gaccaggcgc gccggcgcca gggcagatcg ggatcgtcgt cgacctcggc
     3121 ggggcgccgc accagcgtga tgggcaggtg cagcgggccg gcgccggtgg ggcgccgatc
     3181 acagccaaag ccgaacagtt caaagcgata cggccccacg ggcagcggcc agccgtcggc
     3241 gtcatgccca tgccagacga gggtgttggc cccggcccgg atctggatct cgcccaggct
     3301 cagcacccag gtgccctgcc cgttgaagac aaccagcgtg gcgtagccat cctcggcggc
     3361 gtcaaagtag atgcgcacct gctcgctgtc cgggtcgaac tgcgagcggc tggcgcgcaa
     3421 attggctaac tgctgggcca tgctggggcc tcctcggtag tatcacgacc gcctcagggt
     3481 ggtggaaggc agcgtgtcgt cgatctgagg ctactttagc aaggcgttgt cgtaaatcta
     3541 tcgaaaaagc agcgggggtt gcggaagggc ccggcgacat tttcgcaggc cagggggtca
     3601 ggcgaggcgc ccggtccggc gcagggcggc gagatcgcgc tggatggtgc gcagcgacac
     3661 gccgaaactg cgggccagcg cgccgtcgtc cagggcgagg ccgcgctcgg cggcgtcgtg
     3721 gagcatgatc agcagttcgg cccggcggcc cgccaccttg cgctcgactt cgggggcatc
     3781 catgctgtca tcatcaggcc cgccgggcag gccgtccggc gcggcgacgc gagcctccca
     3841 ggcggcgcgc agcacccggc actcgcgcac gccctccagg taggaggggc gcaggaaggc
     3901 gccacactga tccgccgtcg ccatcatgtg ggcgtagccg cgctccagca cggcgccggc
     3961 ttctgctgtg gggctggacg cggcgaggac ggtgtagcaa tgggccagca cttcgaccga
     4021 gggctcagcc cagctgagcc ggctcggcgg cggcccggca tcgagcgcgg ccagggcgtc
     4081 ctgcgcggcg tggcgcgccg ccccggcacg gcgcagcccc aggtgcgccc gcgccatctg
     4141 ggcaggaaag accggggcat actggccgcc cgcgggccgg cccaggcgtt gccagagcgc
     4201 atggctgcgc tccaccacgg tcagcccggc gctaaaactg ccctggtcca gcaaatggtc
     4261 gcccagccag agcagcgcct gcatggtcga gaaccgggcg cccagcccct ccagcatgcg
     4321 cacggcttct tccaggtcgg cgcgcgccgt gccgggatcg ccgagcacgg cctggatctg
     4381 gccgcgggtg cccagcaaca gggcgatgtc gtggcggctg ccggcggtgc gggccagcgc
     4441 cagggcctgg tcgagatgcg cctgcgcctc ggcatagcgc ccgaagcggg ccagcagctc
     4501 ggccaggttt tgatgcgccg tctcctgcaa gggcagcgat ccggtttcat cggccagggc
     4561 caggaagcgg gtcagcaagt cgtagctggt ggccggggcg ccgacataca gcgccaggtt
     4621 gttcaagaga cgcgcttcca ggtgcaccgc ctggggcacc tggcgcactt tagccaggcc
     4681 ctcttcgagc aaggccaccg cgcggtcgag ttcaccgatc tcgccataga ccggtcccag
     4741 caagctgagg atctcgacct cgccgcgcag atcgcccagg tcgcggtagc ggaccagggc
     4801 gcgctcggcg gcggcaatcg ccgcccggtg gtcgcccagg cggatctgca cccgcgtcac
     4861 ctgggtgaga atgcgggcga ccgcggcgct gttatggccg gcctcggcca gggccagcgc
     4921 ggcggccagg tcggcctgcg cctcggcgta gcgggcctgg gagatgaaat gggcgctgcg
     4981 gcgggccagc gcgtcggcca cccgccccgg ctggttgccc gtgcgggcca gggcgacgag
     5041 ccgatcaagg tccgccgtgg ccgcctccat gcgctccagg cgtttgttga tcgcggcccg
     5101 ccccgccaca agcgcataga cacgctcgat gtcctcctca ccgatgcccg ccggcaggcg
     5161 ctcgacggcg gccagggcct ggtcgtagaa ggccagcgcg gcgtcgttgg cgaacgcggc
     5221 gaccgcgcgc tcggcggctt cttgcaggta aggcagggcg cgcagcgggt cgtctgccgc
     5281 cagccagtga tcggccagcc ggtctaccgc ctgcgggtgc tcggcttcga gcgcgcccgc
     5341 ggcctgccgg tgcaggtggc ggcggcgcgg cccgctgatg ccggcatagg ccacttcgcg
     5401 caccttctgg tgggcgaagg catagcctcg cccggcctcg tcttcgaccc agagttggtg
     5461 gcgcaacaat tcctccgcgg gaccgagcac ggcctcgggc gtcgagccac tggcccgcca
     5521 gagcagcgtt tccggcacct cgcgccccag caccgcgccc acctccagca cgctgcgcgc
     5581 ggcgggcgtc aggcgcgcaa gccgctgttc gagcactcgg cgcacgctgg ccggcgtggg
     5641 cagcgcagtg tagtcggcgc tgttggtgtg ccaggcg
//
LOCUS       DASUYC010000260         5365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_874524,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000260 DASUYC010000000
VERSION     DASUYC010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5365
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874524"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            399..710
                     /locus_tag="VF276_04190"
     CDS             399..710
                     /locus_tag="VF276_04190"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEX6606094.1"
                     /translation="MSNFGLYGKIVTQPGRRDELVAILLEAAAGMPDVPGCDLYIINV
                     SPTEPDAIWVTEVWQSREDHAASLTRADVQALIARGRPLIAGGERIEVVPVGGKGLPA
                     A"
     gene            875..1366
                     /locus_tag="VF276_04195"
     CDS             875..1366
                     /locus_tag="VF276_04195"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX6606095.1"
                     /translation="MTSIAKSVTVALFHQNTWANLQLIDACAALPAEQLDLTVPGTFG
                     TIRHTLTHLVANEEHYLSLLSGRPVEKPIQRGEMLTLAELRARAEAAGAGLAREAAHV
                     GMDDTVAQEYQGQMLTVPAYIVLTQAIQHGAEHRGHIATILSQHGITPPAIDGWAYGE
                     AVG"
     gene            1381..2055
                     /locus_tag="VF276_04200"
     CDS             1381..2055
                     /locus_tag="VF276_04200"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6606096.1"
                     /translation="MDRSAALQRSYDQVTPEYAARFFDELAHKPLDRALLDSFAEQVP
                     RSGPVADVGCGPGQIGRYLWGRGLDVVGVDLSPAMVAQAAQLTPEIAFRQGNMLALDV
                     PDGAWAGIVAFYSAIHLAPSELPRGLGEFYRVLQPGGLLLLSFHIGTEPVHLDEWWGY
                     AVDLDFYFYDRATVEAALAGAGFTLAAYVERPPYAPLEHPTRRMYLLARKPVNSPRRN
                     EGREDY"
     gene            2139..2681
                     /locus_tag="VF276_04205"
     CDS             2139..2681
                     /locus_tag="VF276_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HEX6606097.1"
                     /translation="MITAEQQEAARRRSREYLERAGIVLTPQEAANIEIADMGLGELE
                     TTGLQLVVYVNTQRCCAKELVLFPRQTCPEHRHPPVGGEPGKEETFRCRWGLVYLYVA
                     GPPVADPQARPPAGRENTYTVQHEIVLHPGEQYTIPPDTLHWFQAGDEGAVVSEFSTR
                     STDEYDIFTDPAIRRAPEVG"
     gene            2972..4765
                     /locus_tag="VF276_04210"
     CDS             2972..4765
                     /locus_tag="VF276_04210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX6606098.1"
                     /translation="MSTPVPVPGAAATVPDSVRWQAALLEQIHQVVIAFDPAGRLLLW
                     GRGAAALLGATPPPDLDPATWAVASQAWYWAGPDTEGALIALAGRVYPVADDQGRRIA
                     CLVGADLAPPARPALPTGAALYRWIVESAQEGIWLIDAAGRTTFANARMAGLLGYSRA
                     EMFGRTIYDFMDAPARAEAAQNLERRRPGVTEQHEFRLRRKDGRYCWTLMSTNPLHGS
                     DGAFLGALALVTDISARKQAEEALRQSEQRFAAVFHGSPDAISITRRADRQVCDVNES
                     WERLFGYSRGEMVNPAVDAISLWSDSASRDRVRTELRARGEVRDLPVALRHKSGRMFQ
                     AAVSVEILAIEQEEYLLSVCRDISAQQEMEERLLAAERLSALGRVAAGVAHEFNNVLA
                     GIMGRLDLLALQPADPATAATLGEIQAAAEAGAQVVARIQALARLRAPSPPAVVPIAP
                     LIQEVVGTLQPAWQAQRAPGAPPLTVHTVVDHGLAVHGTAAELRELLTHLLQNAVDAV
                     PPGGEIAVTAAAEPPAVLLTVRDSGPGIAPADQGRVFEPFFTTKITGSGLGLTAVRDI
                     VARHGGAITLDSAPGAGTTFRILLPAAPGEC"
     gene            5101..>5365
                     /locus_tag="VF276_04215"
     CDS             5101..>5365
                     /locus_tag="VF276_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606099.1"
                     /translation="MSRSFRQPNTGDGASDAAASREQMRAEVAGWQARLAAREQAEAR
                     ARCLAELSRRALAEADPAPVLAAAVAGVAAMLDVAYSAVLEYLP"
BASE COUNT          815 a         1892 c         1871 g          787 t
ORIGIN      
        1 gggcgggcta cgtgaacgaa ggccgcctgc gcggcctgcc tgggataacg agtgcggagt
       61 gccgaataac gacgagacgg cgagagacgc cgtaggggcg gaccagctca gtcgctcctc
      121 cgcctgacca ccccgctgcc cccgccgtag ggacggaccg gcgtgtcgcc cggccgccct
      181 acggccccca tcgccccagc ggcgccccag cccgcgcagg cgggcttcgt taccgtagcc
      241 cgccccttga ggggccgggc ggcgccgggg cggggatcgg gcggcgcccg gatgccgccc
      301 atgctcgaca ggccccggcg tttgtgatac gatcaacccg gcgcgccgat gcgccttgta
      361 ggggccggtt tcaaaccggc tcgcccagga aggagcctat gagcaacttc ggattatacg
      421 gcaaaatcgt cacccagccg gggcggcggg atgaactggt agcgatcctg ctggaggctg
      481 ccgctggcat gcccgacgtg cccggttgcg acctctacat catcaacgtc tcgcccaccg
      541 agccggacgc catctgggtc acggaggtct ggcagagccg cgaggatcat gcggcgtcgc
      601 tgacccgcgc cgatgtgcag gcgctgatcg cgcggggccg gcccctcatc gcgggcggcg
      661 agcgcatcga ggtcgtgccc gtgggcggca agggtctgcc cgcggcgtag gccgccgccc
      721 gcggcgacga gactcttcgc tacgctcaga gtgacaagcc caccaactgt cattctgcgc
      781 gtagcgaaga atctcgtctc ggttggatcg ggcgagactc ttcgctgcgc gcagagtgac
      841 aagatgccct tgtccataag gaggtttgcg catcgtgaca tccatcgcga aatcggtgac
      901 ggtggcgctg ttccaccaga acacctgggc caacttgcaa ctcatcgacg cctgcgcggc
      961 gttgcctgcc gagcagttgg acctgaccgt gccgggcacg ttcgggacaa tccgccatac
     1021 cctgacgcat ctcgtggcga acgaggagca ctatctgtcg ctgctcagcg ggcgaccggt
     1081 cgagaagccg atccagcgcg gagagatgct gacattggcc gaactgcggg cgcgggccga
     1141 ggcggcgggc gccgggctgg cccgcgaggc ggcgcacgtg ggcatggacg acaccgtggc
     1201 gcaggagtac cagggccaga tgctgacggt gcccgcctac atcgtgctga cgcaggcgat
     1261 ccagcacggc gccgagcatc gcggccacat cgccacgatc ctgtcgcagc acggcatcac
     1321 gccgcccgct atcgacggct gggcctatgg cgaggccgtc gggtagcgcg gggaggccgc
     1381 atggatcgca gcgcggcgtt gcagcgcagc tatgaccagg tgacgccgga gtacgcggcg
     1441 cggttctttg acgaactggc gcacaagccg ctcgaccggg cgctgctcga cagcttcgcc
     1501 gagcaggtgc cgcggtcggg gccggtggcc gatgtgggct gcgggccagg gcagatcggg
     1561 cgctacctgt gggggcgcgg gctggacgtg gtaggcgtag acctgtcgcc ggcgatggtg
     1621 gcccaggccg cgcaactgac gccggagatc gccttccgcc agggcaacat gctggccctc
     1681 gacgtgcccg acggcgcctg ggcggggatc gtcgccttct attcagccat ccacctggcg
     1741 cccagcgagt tgccgcgcgg cctgggcgag ttttaccgcg tgttgcagcc gggcggcttg
     1801 ctgctgctca gcttccacat cggcacggag cccgtccatc tcgacgagtg gtggggctat
     1861 gccgtggacc tcgatttcta cttttacgac cgggcaacgg tcgaagccgc gctggcgggg
     1921 gcgggcttca ccctggctgc ctacgtcgag cgcccgccgt atgcgccgct ggagcacccc
     1981 acccggcgca tgtatctgct ggcccgcaag cccgtaaatt caccgcgaag gaacgaagga
     2041 cgcgaagatt attgatatcg ggtttgttct tcgcgctctg cgcgtcttcg cggtgattgg
     2101 ataagcaggt gtggtgtaaa gaggaggagc atgttgttat gattaccgct gagcaacagg
     2161 aggcggcccg ccgccgcagc cgcgagtacc tggagcgcgc cggcatcgtg ctgacgccgc
     2221 aggaggcggc caacatcgag atcgccgaca tgggcctggg cgaactggag acgaccgggc
     2281 tgcaactggt cgtgtatgtc aacacgcagc gctgctgcgc caaggagctg gtgctgttcc
     2341 cgcgccagac ctgcccggag catcgccacc cgcccgtcgg cggggagccg ggcaaggagg
     2401 agacgttccg ctgccgctgg ggcctcgtct acctctacgt ggcgggtccg cccgtggccg
     2461 atccgcaggc gcggcccccg gcggggcgcg aaaacacgta caccgtgcag catgagatcg
     2521 tgttgcatcc cggcgagcag tacaccatcc cgcccgacac gctgcactgg ttccaggcgg
     2581 gcgacgaggg cgccgtggtc tccgagttct ccacccgcag caccgacgag tacgacatct
     2641 tcaccgaccc ggccatccgg cgcgcgcccg aagtcgggtg aggcgcccgc cactcgtcct
     2701 tgtcactctg agcgcagcga agagtctcgt cttgtagccg agcgagacga gattcttcgc
     2761 tgcgctcaga atgacaacgt aggctggaag ccatgcggag gcgcccgcgc cgcaaaacag
     2821 ggccggtggg taaggtttgg ttagggacca aagtccattg ccgccccgcc gcggaaaatg
     2881 ttacaactag ggcaattatg ctaacctgcg cgacctaccc tccgcccatg ccggggccgg
     2941 ggtgcgcgcc gactgcccag gcggcccgac catgtctaca cccgtgcccg tgccgggcgc
     3001 cgctgccacc gtaccggact cagtgcgctg gcaggcggcg ctgctggaac agatccatca
     3061 agtcgtcatc gccttcgatc ccgcgggccg ccttctgctg tgggggcggg gcgcggcagc
     3121 cttgctgggg gcgacccccc cgcccgatct cgacccggcc acctgggccg tcgcctccca
     3181 ggcctggtat tgggccgggc ccgacaccga gggcgcgctg atcgccctgg ccgggcgcgt
     3241 ctacccggtg gccgacgacc agggccggcg catcgcctgc ctggtgggcg ccgacctcgc
     3301 gcccccggcc cggcccgccc tgcccaccgg cgcagccctc taccgctgga tcgtggagtc
     3361 ggcgcaagaa ggcatctggc tgatcgacgc cgcggggcgc accacgttcg ccaacgcgcg
     3421 tatggccggc ctgctgggct acagccgcgc cgagatgttt ggccgcacta tctacgattt
     3481 catggacgcc ccggcccgcg ccgaggccgc acagaacctg gaacgccgcc gccccggcgt
     3541 caccgagcag catgagttcc ggttgcggcg caaagacggg cgctattgct ggacgctcat
     3601 gtccaccaac cccctgcacg ggtccgacgg cgcgtttctc ggcgcgctgg ccctcgtaac
     3661 cgatatcagc gcgcgcaagc aggccgaaga agcgctgcgc cagtccgaac aacgcttcgc
     3721 cgccgtcttc cacggcagcc ccgacgccat cagcatcacc cgccgggccg accggcaggt
     3781 gtgcgacgtg aacgaaagct gggagcgcct gttcggctac agccggggcg agatggtgaa
     3841 cccggcggtg gatgccatca gcctctggag cgacagcgcg agccgcgacc gggtacggac
     3901 ggaactgcgc gcgcgcggcg aggtgcgcga tctgcccgtg gcgctgcggc ataaatcggg
     3961 ccggatgttc caggccgccg tgtcggtcga gatcctggcc atcgagcagg aagaatacct
     4021 gctgtccgtc tgtcgcgaca tcagcgcgca gcaggagatg gaggagcgcc tgctggcggc
     4081 ggagcggctg agcgcgctgg gccgggtggc ggcgggcgtg gcccacgagt tcaacaacgt
     4141 gctggccggc atcatgggcc ggctcgatct gctggccttg cagcccgcgg acccggccac
     4201 ggcggccacc ctcggcgaga tccaggccgc cgccgaagcc ggggcgcaag tcgtcgcgcg
     4261 tatccaggcg ctggcccgct tgcgcgcccc cagcccgccc gccgtggtgc ccatcgcccc
     4321 cctcatccag gaggtggtcg ggacgttgca gccggcgtgg caggctcagc gcgcgcccgg
     4381 cgccccgccc ctgaccgtgc ataccgtggt ggatcacggc ctggccgtcc acggcaccgc
     4441 cgccgagttg cgcgaactgc tgacccacct gctacagaac gccgtggacg ccgtgccgcc
     4501 gggcggcgag atcgccgtca ccgccgccgc cgagccgccc gccgtgctgc tgaccgtgcg
     4561 cgacagcggc cccggcatcg cccccgccga ccagggccgg gtgttcgagc ccttcttcac
     4621 caccaagatc accggcagcg gcctggggct gaccgccgtg cgcgacatcg tcgcccgcca
     4681 cggcggcgcc atcaccctcg actccgcccc cggcgcgggc accacgttcc gcatcctcct
     4741 gcccgccgcg cctggcgagt gctgagtgct gagtgctgag tgctgagtga cgacgagtgg
     4801 tcagtggtca gtggtcagtg atcagtggtg agccgtcagc cgtcccgtct tagccgtcag
     4861 ccgtgtgcta cgcaccgctg cgcgatcagc cgtcagccgc caagggtcaa tggccgggga
     4921 tctgtcattc tgagcgcagc gaagaatctc cgtctcgttt ggggccgcgc cgagactctt
     4981 cgctgcgctc agagtgacaa gcacccagcc gttccccatc tcctgaccac tgaccactga
     5041 ccactgacca ctgaccactg acccctcgcc agcgccgccc ggatggtgta aactggggcg
     5101 atgagccgca gtttccgcca accgaatacg ggcgacggcg cgagcgacgc ggcggcgagc
     5161 cgggagcaga tgcgggccga ggtggccggg tggcaggccc ggctggcggc ccgcgagcag
     5221 gccgaggcgc gcgctcgctg cctggccgag ttgagccgcc gcgccctggc ggaggccgat
     5281 cccgccccgg tgctggccgc cgccgtcgcc ggcgtggccg ccatgctcga cgtggcgtac
     5341 agcgccgtgc tcgaatatct gcccg
//
LOCUS       DASUYC010000261         3264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_519160,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000261 DASUYC010000000
VERSION     DASUYC010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519160"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..322
                     /locus_tag="VF276_04220"
     CDS             38..322
                     /locus_tag="VF276_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606100.1"
                     /translation="MRSSTIQAISEFEAGVQVGQTVWVRFTSGYRFFNTPATVVRVSP
                     KSFSVTTNAALADGTAYATPQGTRVNVPRFQPTARNGWSANNRIAPAQEV"
     gene            327..584
                     /locus_tag="VF276_04225"
     CDS             327..584
                     /locus_tag="VF276_04225"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX6606101.1"
                     /translation="MGINGKRIRERRKELGWTLAELARQVGINPAHLWRIETGAKGGG
                     NPSAKTLRRFAEVLGLSESELLTPVEPDVDENAPQELALAG"
     gene            1148..1918
                     /locus_tag="VF276_04230"
     CDS             1148..1918
                     /locus_tag="VF276_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606102.1"
                     /translation="MLTDIIPEPAQDCNYRPMYHAPCPHCRANCRYSVATHRVTCPGC
                     GWQVQLSPAEAQDLLWGLAGCSTLDLGAFLAVLATRCAPAPEAPPAVFSAGGRGVLTQ
                     RVAPAPDETPRQRAERLAEQYSLNGGRLRFADGVVYATSQQRRESDLAPLAEYTITRV
                     AGRDFTCNCRHAGLCAHLAAYWAHLCTKLTDPDQRAALSTTQYERTTLTLNHLELQFG
                     IAARGQNPAEGNPIPPPPPTERPRATVVDLTTELYGAA"
     gene            2056..2403
                     /locus_tag="VF276_04235"
     CDS             2056..2403
                     /locus_tag="VF276_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606103.1"
                     /translation="MSVETNRTCKHCGLSYNVFNDVGSTGSYCTMCNAPTLIVVAFYG
                     QRNSETAAHTYALRQANAFFAEFGIGREQFVFPPTTNTAPDTNEDWGAYTITYHLEVP
                     AYVAQQIREARNA"
     gene            2396..2893
                     /locus_tag="VF276_04240"
     CDS             2396..2893
                     /locus_tag="VF276_04240"
                     /inference="COORDINATES: protein motif:HMM:NF024784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX6606104.1"
                     /translation="MPNPTPAALFPPRFAAKIAVNSETGCWEWQGAIQSRGYGSTDLR
                     LADGRRLSLAHHAAYVYATGQTVLPTCCLLHICDNRRCVRPDHLRVGTVADNNADCRA
                     KGRHAHGTRHPKAKLSPAIIAAARERAALGGIPFVHLARVCGVSAPTLRDALAGRTWA
                     ESEAA"
     gene            2893..3024
                     /locus_tag="VF276_04245"
     CDS             2893..3024
                     /locus_tag="VF276_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606105.1"
                     /translation="MPFFLVLGIVCILCIWAGLCWLSPAARDVTWGAPSNESGHFDE"
     gene            3017..>3264
                     /locus_tag="VF276_04250"
     CDS             3017..>3264
                     /locus_tag="VF276_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606106.1"
                     /translation="MSRIAVQGSVVSSIAPRLGLARQIERILADHLTRFAGVPEAVYL
                     PDPRGLCEVTVNGLTIPIRAGWPTDPEARRRAYLADHAV"
BASE COUNT          588 a         1113 c         1057 g          506 t
ORIGIN      
        1 gcatagaagc aacaaacaca gtgaaggagc accggcgatg cgcagcagca ctatccaggc
       61 gatcagcgag tttgaggcgg gcgtgcaagt ggggcagacc gtttgggtcc gcttcacgag
      121 cggctaccgc tttttcaaca ccccggccac ggtggtgcgc gtttctccga agtccttctc
      181 tgtgaccacg aacgcggcgc tggccgacgg caccgcgtac gccacgccgc agggcacgcg
      241 ggtcaacgtc ccgcgctttc agccgacggc gcgcaacggc tggtcggcca ataaccggat
      301 cgcgccggca caggaggtct aacgcgatgg ggatcaacgg caagcgcatc cgcgagcggc
      361 gcaaggaatt gggttggacg ctcgcggaac tggctcgaca agtgggcatc aacccggccc
      421 acctatggcg gattgaaacc ggggccaagg gcggcggcaa ccccagtgcc aaaacgctgc
      481 gccggtttgc cgaggtgctg gggctctcgg aatcggaact actgaccccg gtggaaccgg
      541 acgtagacga gaacgcgccg caagaactgg cgctcgcggg atagccatga cccctgagca
      601 aatcgcccgg ttttgggcca aagtcgataa gtccggtgag tgctggattt ggacgggcgc
      661 ctgtaatcga cagggttatg gacttgcctg gaatggtcgc actactcgcc ttgcccatcg
      721 ccttgcctgg gaactggtac acggcccgat cccgcccggc aagaatgtct gtcacgattg
      781 ccccggcggc gataatccgg catgtatccg gcatctgtgg ctgggtactc agcaagagaa
      841 tatgcgggat tgtttccgca aagggcgcgt gtcgaatgcg cctaaaccgc gcggtatcta
      901 tatgaccatc gcagtagccc gcgaaatgcg cgcgatgcac gccgccgggg ccactatcgc
      961 gcagatcagt cgcaagtaca atctgtgcct tcaggctgtc agtggcgtag tcagaaaccg
     1021 cacttggcag gaatcgactg aagtatcccc gatatacaga agccgatacc agacggacct
     1081 ttaacccata gaaatgcccc ggcgctgcta cgaacagccc agggcgtgaa gcaaaggaac
     1141 gacccacatg cttaccgaca tcatacccga acccgcacag gattgcaact accggcctat
     1201 gtaccacgcc ccgtgcccgc actgccgggc gaactgccgc tacagcgtcg ccacgcaccg
     1261 cgtgacgtgc cccggctgcg gctggcaggt gcaactcagc cccgccgaag cccaggattt
     1321 gctctggggc ctggccggct gtagcacgct ggacctgggc gccttcctgg ccgtgctggc
     1381 aacccgctgc gccccggccc ccgaggcgcc gccggctgtc tttagcgcgg gcgggcgcgg
     1441 cgtcctcacg cagcgcgtgg ccccggcgcc ggacgaaacg ccccggcagc gggccgaacg
     1501 cctggccgag cagtacagcc tgaatggcgg gcggctgcgg ttcgcggatg gcgtcgtcta
     1561 cgccacgtcg cagcagcgcc gggagagcga cctggcgccc ctggcggagt acaccattac
     1621 ccgcgtggct gggcgggact ttacctgcaa ctgccggcac gcgggcctgt gtgcccacct
     1681 ggccgcctac tgggcacacc tgtgtaccaa actgacggac ccggaccagc gggcggccct
     1741 gagcaccacc caatacgaac ggacgaccct gaccctgaac catctggaat tgcagtttgg
     1801 gattgcggcg cgggggcaga acccggcgga ggggaacccc atcccgcccc cgccgccgac
     1861 ggagcgcccg cgggctacgg tggtggatct gacgacggaa ttgtacggag ccgcctgaca
     1921 tcgggcgatc cccttgagag gtacgggcgc cggccttggc tgccggcgcc ccacgaagca
     1981 acactcgttg gctagtgcgg catctggggt aagaatatct cgccgcgcag cccttgtcaa
     2041 gcaaggagta acccgatgtc tgtcgaaacc aaccgcacgt gcaagcactg tggcctctcc
     2101 tacaacgtgt ttaacgatgt tgggtccacc ggcagctatt gcacgatgtg caacgcgccc
     2161 acgctgatcg tcgtcgcctt ttatgggcag cgcaacagcg aaaccgcggc gcacacgtac
     2221 gctctccggc aagctaatgc cttcttcgcg gaattcggca tcgggcgcga gcagttcgtg
     2281 ttcccgccca cgaccaatac cgccccggat acgaacgagg actggggcgc ctacaccatc
     2341 acctaccacc tggaagtgcc ggcgtatgtg gcccagcaga tccgcgaggc ccgcaatgcc
     2401 taaccccacc cccgccgccc tgttcccgcc gcgcttcgcg gccaagatcg ccgtgaacag
     2461 tgagaccggc tgctgggagt ggcaaggcgc tattcagagc cggggctatg gatcgaccga
     2521 tctgcggctg gccgatgggc ggcgcctctc gctggcccat cacgcggcct atgtgtatgc
     2581 cacggggcaa acggtcctcc cgacctgttg cctactccac atctgcgaca atcgccggtg
     2641 cgtgcgcccg gatcatctgc gggtcggcac cgtcgccgac aacaacgcgg attgccgcgc
     2701 gaagggccgc cacgcccacg gcacgcgcca ccccaaagcc aagctcagcc cggccatcat
     2761 cgcggcggcg cgggaacggg cggccctcgg cggcatcccg ttcgtgcatc tggcgcgggt
     2821 ctgtggcgtc agtgccccca cgctgcgcga cgcgctggcc gggcgcacgt gggcggaaag
     2881 cgaggccgcg tgatgccctt cttcctcgtc ctggggatcg tgtgcatcct ctgcatctgg
     2941 gcgggcctgt gctggctctc ccccgccgcg cgggacgtga cctggggcgc ccccagcaac
     3001 gaatcggggc acttcgatga gtaggatcgc cgtgcaaggc agcgtggtca gcagcatcgc
     3061 gccgcgcctc gggctggccc ggcaaatcga gcgcattctc gccgaccacc tgacgcgctt
     3121 tgcgggcgtg cccgaggcgg tctacctgcc cgatccgcgc gggctgtgcg aagtgaccgt
     3181 caacgggctg accatcccga tccgggcggg ctggcccacg gacccggaag cgcggcgccg
     3241 ggcgtacttg gccgatcacg ccgt
//
LOCUS       DASUYC010000262         3942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1866844, whole genome shotgun sequence.
ACCESSION   DASUYC010000262 DASUYC010000000
VERSION     DASUYC010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3942
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1866844"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..2225)
                     /locus_tag="VF276_04255"
     CDS             complement(168..2225)
                     /locus_tag="VF276_04255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX6606107.1"
                     /translation="MITTTDTTKTAVLSGRLTRDSVLAWLGLAGPLVVSAVLNLWNLA
                     QNGYDNLYYSVAVQSMLQSWHNFFFAAYDAGGFISVDKPPVALWIQTLSAKLLGFNAL
                     GILLPQALAGIASVGLLYYLVRRLFGPLAASVAALALALTPIAVAVERGNNTDTWLMF
                     TLLLAAWAVTRATETGRFALLALSLALVGVAFNIKMLEAFIVLPAFYLLYLVAAPIGW
                     GKRLLHLTLGTVVVLGVALSWPLAVDFTPPDQRPWVGGSQHNSVLELALGYNGLSRVT
                     GQGEGFGGGTRGGFRGGAPGGFPNFGNNPGGFSNLGNAPGGPRGGGGLFDAGNPGPLR
                     LFTGALAGQWTWLLPLALFGLPLAVAGLRRRRPLEGRGQAILLWTGWLAMYGVVFSMA
                     AGIFHPYYLIMLAPAAAALVGIGVGALWAAYRRGGAAAWLLPAALLVTAIWQMTLVGA
                     YPAWSAWLTPLAWGGSILAAVPLVVTRLLSRRVWRRWAPGLVGVGLVALLMAPLAWAV
                     TPVLAAPTNASLPEAGPQALNRYDPTSWTQLYATGGPQPDAISGTGLVAYLEAHSAGY
                     FYLVAVPTSQQASSLALETGKPVLAAAGFMGTDPALTADKLAAMVADKQVRFVMGLGA
                     GGMRGGFGGGSADSGAGAWIQSHCTAVDPSLYQGQIGGTGLRGGFGSFRGGNSQLYDC
                     AAQ"
     gene            2571..2975
                     /locus_tag="VF276_04260"
     CDS             2571..2975
                     /locus_tag="VF276_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606108.1"
                     /translation="MPIFKHFDLTALRRDPQIAYYFRYPLYHSDFQAIRANGRLLGYA
                     AAKPLYGRLTAAGQVDRSAGFNGQIAVLFIKSPARSARRAEILLTAVPPAELTRPDGK
                     RNWPVIRAAAEATVRAHVPPPPDLAPSGARPW"
     gene            complement(3402..>3942)
                     /locus_tag="VF276_04265"
     CDS             complement(3402..>3942)
                     /locus_tag="VF276_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX6606109.1"
                     /translation="DVRSSALWLPVRIFTGWQWIDASLHKLQSPAWMDTGAALKGYWT
                     NAVAIPAQGSPAITYSWWRSFLQTMLDAQAYTWFAKLVAIGEFLIGLGLIVGALVGIA
                     AFFGVLMNTSFLLSGSASTNPVMLLLAIGLILAWKVAGWIGVDRYLLPLLGTPWHRGR
                     LFQGGTRGSKTSPIGAARP"
BASE COUNT          680 a         1460 c         1268 g          534 t
ORIGIN      
        1 atgaccaggt tcctcgccgc cctgcgcttc cgcgcctcca cctgaaccgg cgcctccgta
       61 accccgcata tatctgacag gccagggaga tcccctggcc tatctgcttt cgctgggcga
      121 ccctggcgcg ggccgtccgc cggctgcggt gatccgcagg agatgcgcta ttgcgcggcg
      181 cagtcgtaga gctgcgagtt gccgccccgg aagctgccga acccaccgcg caggccggtg
      241 ccgccgatct gcccctgata gaggctggga tcgaccgcgg tgcaatgcga ctggatccac
      301 gcgccggcgc cgctatcggc gctgccgccg ccgaacccgc cgcgcatgcc gcccgcgccc
      361 aagcccatga caaagcgcac ctgtttatcg gcgaccatgg ccgccagttt atcggcggtc
      421 agggccgggt cggtgcccat gaagccggcg gcggccagta ccggcttgcc ggtttccagc
      481 gccagcgacg aggcttgctg cgaggtgggc accgccacca ggtagaaata gcccgcgctg
      541 tgagcctcca ggtacgcgac caggccggtg cccgagatcg cgtccggctg cggcccgccc
      601 gtcgcgtaga gttgggtcca actggtcgga tcgtagcggt tcagcgcctg cggcccggct
      661 tccggtaggc tggcgttcgt gggcgcggcc agcaccgggg tcacagccca ggccagcggc
      721 gccatgagca gggccaccag ccccacgccc accaggccgg gcgcccagcg ccgccacacc
      781 cgccggctca gcagccgcgt caccaccagc ggcacggcgg cgagaatgct gccgccccag
      841 gccagcggcg tgagccacgc gctccacgcc ggataggcac ccaccagggt catctgccag
      901 atggccgtca ccagcagcgc cgccggcagc agccaggccg ccgcgccgcc gcggcggtac
      961 gccgcccaca gcgcgcctac gccgatgccc accagggccg ccgccgcggg cgccagcatg
     1021 atcaggtagt acgggtggaa gatacccgcg gccatgctga agaccacgcc atacatcgcc
     1081 agccagccgg tccagagcag aatggcctgg ccgcgcccct ccaggggccg gcgccgccga
     1141 agccccgcca ccgccaacgg cagcccgaac agggccagcg gcaacagcca ggtccactgc
     1201 ccggccagcg ccccggtgaa cagccgcaac ggccccggat tgcccgcgtc gaacaggcct
     1261 ccgccaccgc gcggcccgcc cggcgcattg cccaagttcg agaagccacc ggggttattg
     1321 ccgaagttgg ggaagccgcc cggcgcgccg ccccggaagc cgccgcgggt accgccgcca
     1381 aagccctcgc cctggcccgt cacccggctc agcccgttgt agcccagtgc cagttcgagc
     1441 acgctgttgt gctggctgcc gcccacccag ggccgctgat cgggaggcgt gaagtccacc
     1501 gccagcggcc acgacagcgc cacgcccagc accaccacgg tgcccagcgt caggtgcagc
     1561 agccgcttgc cccacccaat cggcgcggcc accaggtaca gcagatagaa cgctggcagc
     1621 acgatgaacg cttccagcat cttgatattg aacgcgacgc ccaccagcgc caggctcagc
     1681 gccagcagcg cgaaccgtcc cgtttcggtg gcccgcgtga cggcccaggc cgccagcagc
     1741 agcgtgaaca tcaaccaggt atcggtgttg ttgccgcgct ccaccgccac ggcgatgggc
     1801 gtcagggcca gcgccagcgc tgccaccgac gcggccagcg gcccgaacag ccgccgcacc
     1861 aggtaataga gcaggccgac ggacgcgatc ccggccagcg cctgcggcaa gaggataccc
     1921 agcgcgttga accccagcag cttggcgctc aacgtctgga tccagagcgc caccggcggc
     1981 ttatcgaccg agatgaatcc gcccgcgtca tacgccgcga agaaaaaatt gtgccagctt
     2041 tgcaacatgc tctgcacggc gacagagtag tagaggttat cgtagccgtt ctgcgccagg
     2101 ttccacaggt tcagcaccgc ggacaccacc agcggcccgg ccagccccag ccacgccagg
     2161 accgagtccc gcgtcaggcg gcccgagagg accgcggttt ttgtcgtgtc ggtagttgtg
     2221 atcattccca ttgtcctcgg ttgtcccaac aactcgatcc gcgcacccgc gcgccgccgg
     2281 catggtagcg gcacgccccg gcatccgcgc tccgcgtgcg gatagcccca gtgtaacagg
     2341 cgaactttag aacaaggcca ggaacaggta agaaattgat cagactatcg gcccgaagat
     2401 ttgcgggggc gggccggggt gccacccggt cgggctgatc cccctcgtta cgtggaaaga
     2461 caaccaggct gatcacgcag gtggagtcgg ggcacagatt gtgccggggt gataacagca
     2521 gcgcccgcgc cggatagggg agccaggccg cggcacgagg aggaaaagcc gtgcccatct
     2581 tcaagcactt cgatctaacc gccctgcggc gcgatcccca gatcgcctac tatttccgct
     2641 acccgctcta tcatagcgac ttccaagcga tacgcgcaaa cgggcgcctg ctcggctacg
     2701 ccgccgccaa gccgctctac gggcgactga cggccgcggg ccaggtggac cggtcggcgg
     2761 ggttcaacgg ccagatcgcc gtgctcttca tcaaatcccc cgcccgctcg gcccgccgcg
     2821 ctgaaatcct gctcaccgcc gtaccacccg ccgagctaac acggcccgac ggcaagcgca
     2881 actggcccgt gatccgcgcc gccgccgagg ccaccgtgcg tgcccacgtc cccccaccgc
     2941 ctgacttggc gccctccggc gcccgtccat ggtaattacc cttatagagg ccggtttgaa
     3001 accggcccgg cgctacctgg ggatgcgcgc cggcattata gaggccggtt tcgaaccggc
     3061 ccggcgccgt ccgccggcgc tgtggtatac ttatagcgac gggctgattc tcctgcgtcg
     3121 cagcgagatc cgctcgccac ggagatccgc cgcgggaacc ggccaacccg gcgccgcggc
     3181 ccagttgaag cgccgcgggt gcccgttcag gcggcgtcga acctggacca gcaacgggca
     3241 acggcacgcg atagcggcta tcgcgcgggc tgaggctccg ttcgattcgg agaatatttg
     3301 gtagggtaag ccccggcggg ccggttcgac tccggcccgc gtgctctaat tctcactgat
     3361 tatcggcgct tactccagcg ccggcggcta ttgctccctg gtcaaggccg cgccgcgccg
     3421 atcgggctcg tcttgctgcc gcgtgtgccg ccctggaaga ggcggccccg gtgccacggc
     3481 gtgcccagca gcggcagcag gtagcggtcg acgccgatcc agccggcgac cttccaggcc
     3541 aggatcaggc cgatcgccag cagcagcatg accggattgg tcgaggccga ccccgacagc
     3601 aggaacgacg tgttcatgag cacgccgaag aaggcggcga tccccaccag cgcgcccacg
     3661 atcagcccga gaccgatcag gaattcgccg atagccacga gtttggcaaa ccaggtgtag
     3721 gcctgcgcgt cgagcatggt ctgcaggaag ctgcgccacc agctataggt aatcgccggg
     3781 ctgccctggg ccggaatcgc cacggcgttg gtccagtaac ccttcagcgc ggcgcccgtg
     3841 tccatccagg ccggactctg cagcttgtgc aggctggcgt cgatccattg ccagccggtg
     3901 aagatccgca ccggcagcca gagtgcgctg gaacgcacat cg
//
LOCUS       DASUYC010000263         5842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1245488, whole genome shotgun sequence.
ACCESSION   DASUYC010000263 DASUYC010000000
VERSION     DASUYC010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5842
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1245488"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VF276_04270"
     CDS             complement(<1..327)
                     /locus_tag="VF276_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEX6606110.1"
                     /translation="MIQVKRVYEPAEPGDGARFLVERLWPRGMKKAALQMDGWLKDVA
                     PSTALRQWFGHDPARWAEFQRRYDAELAAAPDAWAPLVAAARQGSVTLLYSARDTEHN
                     AALALRD"
     gene            complement(456..794)
                     /locus_tag="VF276_04275"
     CDS             complement(456..794)
                     /locus_tag="VF276_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6606111.1"
                     /translation="MDNAYTYIADLAKEVTVPQDGILSRILYKDAQVNVTLFGFDAGQ
                     ELSEHTAASPAIIQILQGEARLVIAGEEREGRAGTWIHMAARTPHSLIAQTPVVMLLL
                     LLKSPGGSRD"
     gene            complement(829..2514)
                     /locus_tag="VF276_04280"
     CDS             complement(829..2514)
                     /locus_tag="VF276_04280"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011057661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEX6606112.1"
                     /translation="MAENRPRGNAAELLVRCLENEGVEYVFGLPGEEIVDVLDALSRS
                     SQIRLVVTRHEQGAAFMADVYGRVSTYPGVCLATLGPGATNLITGIADANLDRAPLVA
                     ITGQAGLERVHKESHQYLDVVQMLQPITKWNARVARAETLPEMVRKAFRLARLEKPGA
                     THLELPEDVAAEPVDAAIHPLPVRRTTYPAPTAATLEAAAEQIAAAQHPVVLAGNGVI
                     RREASGHDAAAGLAAFAHTLDIPVLPTFMGNGALDARDALAGPAVGLQRPGADLALHP
                     VLAAADLVITAGYDLVEWAPALWNPNRDKTIIHVDSTPAEIDGHYLPAVEVVGEIGAA
                     LSALAALCADQPCRRPERAALAAHPYESLHAFARDPSVPMKPQRVVWELRAALGESDI
                     LISDVGAHKLWLASYYPAYRPNTLIISNGLATMGLAVPGGLAARLAQPTRRVVAVSGD
                     GGFLMNSQELETAQRLRVPYVNVVWSDGRYGVIELNQQRRLGRTFGVEFGNPDLVAYA
                     RSFGLPAWQVEQADDFRPLLQRALELDGPSLIVVPTDARENQRLGQPATAAAE"
     gene            complement(2501..3133)
                     /locus_tag="VF276_04285"
     CDS             complement(2501..3133)
                     /locus_tag="VF276_04285"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX6606113.1"
                     /translation="MPDTPPVTLEAIRGVLQTTPARWQALMAQLPAAILERPAAPGEW
                     SAVDCLRHLIDLETRVFPTRLRALLAGQAFPAAGAPVATAGASPAELAALFARHRTAN
                     LDLLAGVQPADLDRAIAHPQLGQVTVRNLVHDWAAHDLMHTVQAERAMLQPFLPGTGP
                     WRHVFADHDLGDSAPDVAPPPSGAVGAGDVAPPPSGAAGAGKEDTRDGGE"
     gene            complement(3126..3674)
                     /locus_tag="VF276_04290"
     CDS             complement(3126..3674)
                     /locus_tag="VF276_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606114.1"
                     /translation="MIQLDATTLDLVATAPPSDLFERLTRDLRPAVKRLARQIAASET
                     NLAVLAFLRCYPHTALTANDLARQIDRPYVEVAPALAGWVAAALVEDATAGDITFYRL
                     TTDAARLRDLEDVLAWQARWLDQALGIAQAIGVPVLLLRSPVAAVLPETAVAHCQLGH
                     ALNDEYGPYTYAGHGAGGTGDA"
     gene            complement(4125..4415)
                     /locus_tag="VF276_04295"
     CDS             complement(4125..4415)
                     /locus_tag="VF276_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606115.1"
                     /translation="MDGSDFRAATVEKEMETQQSLEGLRESWRTLRRRMPDIHQDTYT
                     VEGLSHLLGIPRDVIAHEITTGQLQALRCGSHTICIERSAVVDWLQRRGPGV"
     gene            4847..5665
                     /locus_tag="VF276_04300"
     CDS             4847..5665
                     /locus_tag="VF276_04300"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX6606116.1"
                     /translation="MALSAAPRLQAVFFDMSKTLWRPASGTRAGRARQAHDALYAALG
                     RYYPTLTLPARDRFSEVLQAIEHRHGPMAVSNEEILEHLRAAFGWMLDPADDRLLTAW
                     HAPKLEQTVQEPDLPQTLDALQALGLRLGVISNTRWRRPWHDRELAQHGLLSYFPVRI
                     YSADLGIRKPDPDIFAAGLTALGELPAEEVLYVGDKLDTDVAGARQAGWRTAWLLPPG
                     KPAPPPTEAIQPDIMLAALRDLPAQLTAHFRLPTRRSLGADHFSMPAASEVPAP"
BASE COUNT          881 a         2108 c         1905 g          948 t
ORIGIN      
        1 atcgcgcagg gccagggcag cgttgtgctc ggtgtcgcgg gcgctgtaga gcagcgtgac
       61 ggaaccttgc cgggccgctg ccaccagcgg cgcccaggcg tcgggcgccg cggcgagttc
      121 ggcgtcatag cggcgctgga actcggccca ccgcgccggg tcgtggccga accactgtcg
      181 cagcgccgtg ctgggcgcca cgtccttgag ccagccgtcc atttgcagcg ccgctttctt
      241 catgccccgc ggccacagcc gctcgaccag aaaccgcgca ccgtcccccg gctccgccgg
      301 ctcatacact cgtttgacct ggatcatccg tccgcccccc gcttcctgtc cgttccaccc
      361 gagtggcgac cctgcccgtc gcccatcgcc atgctgcgct gtaggggccg gtttgaaacc
      421 ggcccggccc gccgcgccaa ctcgctggta gacggctaat cccggctgcc acccggcgac
      481 ttcaacaaca gcaagagcat gaccaccggt gtctgggcga tcaggctgtg cggggtgcgc
      541 gcggccatgt ggatccaggt gcccgcgcgg ccctcgcgct cctctccggc gatgaccagc
      601 cgggcctcgc cctgcaagat ctggatgatc gccgggctgg ccgccgtatg ctcggacaac
      661 tcctgcccgg cgtcgaagcc gaacagggtc acattcacct gggcgtcttt atagaggatg
      721 cggctcagga tgccgtcctg tggcaccgtg acctctttgg cgaggtcggc gatataggtg
      781 taagcgttgt ccatgactac tttcctgtct ccttttcctt ccggtctctt attcggcggc
      841 cgcagtcgcc ggttggccca ggcgctggtt ctcgcgcgca tcggtgggca ccacgattag
      901 cgacgggccg tcgagctcca gcgcgcgctg cagcagcggc cggaagtcgt ccgcctgctc
      961 cacctgccag gcgggcaggc cgaacgaccg cgcgtaggcc accaggtcgg ggttgccgaa
     1021 ttcgacgccg aaggtgcgcc ccaggcgccg ctgctggttc agttcgatga cgccgtagcg
     1081 cccgtcgctc cagacgacgt tgacgtaggg cacgcgcagg cgctgcgccg tctccagttc
     1141 ctgcgagttc atcaggaagc cgccgtcgcc gctgacggcc accacgcgcc gggtgggttg
     1201 cgccagccgt gcagccagcc cgccgggcac ggccagcccc atcgtcgcca gcccgttcga
     1261 gatgatcagc gtgttcgggc ggtaggccgg gtagtacgag gccagccaga gcttgtgcgc
     1321 gcccacgtcg ctaatcagaa tgtcggattc gcccagcgcg gcgcgtagct cccagaccac
     1381 ccgctgcggc ttcatcggca cgctcggatc gcgggcaaac gcgtgcagcg actcgtacgg
     1441 atgggcggcc agggcggccc gctccgggcg ccggcacggc tgatcggcac acagggcggc
     1501 cagggcgctc agggccgcgc cgatctcacc caccacctcg accgcgggca gataatggcc
     1561 gtcgatctcg gcgggcgtgc tgtccacatg gatgatcgtc ttgtcgcggt tgggattcca
     1621 aagcgcgggt gcccactcca ccaggtcgta gcccgcggtg atcaccaggt cggcggcggc
     1681 caggaccggg tgcagggcca ggtcggcgcc gggccgttgc agccccaccg ccggcccggc
     1741 cagggcatcg cgggcgtcga gcgcgccgtt gcccatgaac gtgggcagca ccgggatgtc
     1801 cagcgtgtgg gcgaaggccg ccaacccggc ggcggcatcg tggccgctgg cctcgcggcg
     1861 aatgacgccg ttgcccgcca gtaccaccgg gtgctgcgct gccgcgatct gctctgccgc
     1921 ggcctccagg gttgccgccg tgggcgccgg gtaggtcgtg cggcgcacgg gcagcgggtg
     1981 aatcgccgca tccaccggtt cggcggccac gtcttcgggc aattccagat gcgtggcgcc
     2041 cggcttttcc agccgtgcca gccggaaggc tttgcgcacc atctccggca gcgtctcggc
     2101 ccgcgccacc cgcgcgttcc acttggtgat cggctgcaac atctgcacca cgtcgaggta
     2161 ctggtgcgac tccttgtgta cccgttccag cccggcctgg ccggtgatcg ccaccagggg
     2221 cgcccggtcg aggttcgcgt cggcgatccc ggtgatcagg ttggtcgcgc ccggccccag
     2281 cgtcgccaag cagacgcccg gataggtgct cacccggcca tacacatcgg ccatgaaagc
     2341 ggcaccctgc tcgtgccgcg tgaccaccag ccggatctgc gaactccgcg acagcgcgtc
     2401 gagcacgtcc acgatttcct cgcccggcag cccgaagaca tactcgacgc cctcgttctc
     2461 caggcagcgc accaataact ccgccgcgtt gccgcgcggc ctattctccg ccatcccgtg
     2521 tatcctcctt acctgcgcct gccgccccac tgggcggcgg cgccacatca cccgcgccta
     2581 ccgccccact gggcggcggc gccacatccg gcgcgctatc gcccaggtcg tggtccgcaa
     2641 agacatgccg ccaggggccg gtaccgggca ggaacggctg caacatggcg cgctcggcct
     2701 ggaccgtgtg catcaaatcg tgtgccgccc agtcgtgcac cagattgcgc actgtgacct
     2761 ggccgagctg gggatgcgcg atggcgcgat ccaggtccgc cggctgcacg ccggccagca
     2821 ggtcgagatt ggcggtgcgg tgccgggcaa acagcgccgc cagctcggcg ggcgacgcgc
     2881 cggcggtggc aactggcgcg ccggcggcgg gaaacgcctg ccccgccagc agggcgcgca
     2941 ggcgggtcgg gaagacgcgg gtttccaggt cgatgagatg ccgcaggcaa tccacagccg
     3001 accactcgcc cggagcagcg ggccgctcca gaatcgccgc cgggagttgc gccatcagcg
     3061 cctgccagcg cgcgggcgtc gtctgtaaca cgccgcgaat cgcctctaag gtgacagggg
     3121 gggtatcagg catcgccggt gcctcctgcc ccgtgcccgg cataggtgta gggcccgtac
     3181 tcgtcgttga gcgcatggcc cagttggcag tgggccaccg ccgtctccgg caaaaccgcc
     3241 gccaccggcg aacgcagcag cagcacgggc acgccgatag cctgggcgat gccgagtgcc
     3301 tggtccagcc agcgcgcctg ccaggccagc acgtcctcca gatcgcgcag gcgtgccgca
     3361 tcggtggtca ggcggtagaa ggtgatatcg cccgcagtcg catcttcgac cagcgcagcg
     3421 gccacccacc cggccagcgc gggggccacc tccacgtacg gccggtcgat ctgccgggcg
     3481 agatcgttcg cggtcagcgc cgtgtggggg taacagcgca agaaagccag cacggcgaga
     3541 ttggtctccg acgcggcaat ctgccgggcc agccgcttca ccgcggggcg caggtcacgg
     3601 gtcaggcgct cgaacaggtc ggacggcggc gccgtcgcca ccagatccag tgtcgttgca
     3661 tccagttgta tcatcgggag tacctccttg tccttcccga tcatcctacc actatcggcg
     3721 ttcccggtgt ttgcgctagc acaaacaggg ccgcgttgat ccgcgccggc gtgcggatca
     3781 cccggcacgc cgggcgccgg cttccttcgt cgtcagtttt tcgggatatg tcgacaggcg
     3841 cggggaggcg cttagtaggc tgtcacactc aaattgtcat tcttcgctcc gctcagaatc
     3901 tccgtctcgt agctagaaag acgagactct tcgttgcgct cagagtgaca aaaaccatgt
     3961 gacagactac tgagatgagg gaggttggca acaccgcggt gtattaccgg cgccgggctg
     4021 cccgtttacc ggccggaggc aaccgttcct ggccccaggc gcgggggcac ggcgggcggt
     4081 gcataaggcc atgctcccgc ccggcgcgcc accggggatc gacgctagac ccccggcccc
     4141 cgccgctgca accagtccac gacggcgctc cgctcgatgc agatggtatg cgacccgcag
     4201 cgcagggcct gcaactggcc ggtggtgatc tcgtgggcga tcacatcgcg ggggataccg
     4261 agcagatgcg ataatccttc gacggtatag gtgtcttgat gaatatcggg catgcgccgg
     4321 cgtagcgtgc gccagctttc gcgcaggccc tctaagcttt gctgggtttc catctctttc
     4381 tccaccgtgg ccgcgcgaaa atccgatccg tccatgccgg cgccggggat aacctgacct
     4441 cccgccatcc cgcgccggtg ggcggatccc aggccggcgg ggcgcgcgct cctactatgg
     4501 ttgcatatgg aaccgtttcc attatacatc ccggcgcccg attcttcaag aaagccgccg
     4561 gtacaaaggc gcccggcgtg gcgcgtaaca gcaggggaac gtgctgcacc gcgcctttgt
     4621 catctgagtt tcatcttccc atcatcttcg cgccgtacaa tacagccata caaccacaag
     4681 gtacacggcc ggccctttga ggccaactgc cccagccgtg taccgtgtac tctcccaaac
     4741 atgctgggag ggcaccctcg ctgcccgact cggtcctccc gatatgcgct gtgtccccgc
     4801 ccttctgcgc cccggcgggg agccgtgcgg accgtggagg ttccatatgg ccctttccgc
     4861 tgcgccccgc ttgcaagcgg tgttcttcga tatgagcaag actctctggc gcccggcgtc
     4921 cggcacgcgc gccgggcgcg cccggcaagc ccacgacgcc ctctacgccg cgctcggtcg
     4981 ctactatccc acgctcacct tacccgcccg cgatcggttc agcgaagtgt tacaagcgat
     5041 tgagcaccgg cacggcccga tggccgtcag caatgaggag attttggagc acttgcgtgc
     5101 agcgtttggc tggatgctgg acccggccga cgatcggttg ctgactgcct ggcatgcgcc
     5161 gaaactagaa cagaccgtcc aggagccgga cctcccgcag acgctggacg ccctccaggc
     5221 gcttggtctg cgcctcggcg tgatctccaa tacgcgctgg cgccgcccgt ggcacgatcg
     5281 ggaacttgcc cagcatgggt tgctcagcta ctttccggtg cgcatctact ccgcagacct
     5341 gggcatccgc aagcccgacc cggacatctt cgccgccggc ctgacggcgc tgggcgaact
     5401 gcccgcggaa gaagtgctct atgtgggcga caaactagac accgatgtgg ccggcgcgcg
     5461 gcaggccggc tggcgcacgg cctggctgct gccgcccggc aaacccgccc cgccccccac
     5521 cgaagcgatc caacccgaca tcatgctggc ggcgctccgc gatctgcccg cccaactcac
     5581 cgcccacttc cggctcccta cccggcgcag cctgggcgcg gatcactttt ccatgccggc
     5641 agcatccgag gttcccgccc cgtaggcggc ggaccgccgg gtctctcccg gcagcgcgga
     5701 caccgcggac gaccagcgcg atcacgccgg ccgcccgcgg ttttcttctt ccgccgccat
     5761 agacttacgg gctaggccgt gccggtaggc agcgcaggcc ggctatggtt gcacgctgcc
     5821 accgcttcca ctttatagcc ag
//
LOCUS       DASUYC010000264         4853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1173309, whole genome shotgun sequence.
ACCESSION   DASUYC010000264 DASUYC010000000
VERSION     DASUYC010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173309"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1770
                     /locus_tag="VF276_04305"
     CDS             <1..1770
                     /locus_tag="VF276_04305"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6606117.1"
                     /translation="PARLIRLLGCIVLGLALLTGCDFFAPAPNTTPVPSPPPATTAGS
                     TAPLATTGPASTVAPTPTTPPNVPRGGTLTVRIPHDVTTLNPLFVDKGPGNQDAAAAQ
                     VTGLIFSGLTRLDDQLRPVPDLAESWDVAPDGLSLTFHLRPGVKWQDGSPVTSADVIW
                     TYNTWLNLTGTTTLQYHLRDAVLQIQPADPPESTVRFWLKKPYAPILADLAAPILPKH
                     LLGNVPLDQLAASDFNFKPVGTGPYEFSDHREGENVILTANPNYYGGPPNISRVAFLV
                     APDPQVAVNALSQGTLSLAEVPQAAWEEYARRPGIQSLFTLARYADPSYYFVAFNVRA
                     GHALSDPRLRQAWALALNKEPLVREATGGAGLPIWSDINPASWAFAPDTPRLNNDSAR
                     ARQLLTEAGWTDTNNDGIVDKGGEPLKISLYVRADDPARRKAAELMGPPLAAVGISTT
                     VVPVDFESVINAKIDPLHEPAFDFHAMVMGWDNQSPDPDDYPLFHSSQIRSKSNPNGL
                     NYIGYNSPEYDELSRKARSEYDPVKRAALYAQIQGLLAQDLPYYFLWADQHYMVLNHR
                     VQGPINLASPRYLWNVEKWWIEP"
     gene            complement(1843..3006)
                     /locus_tag="VF276_04310"
     CDS             complement(1843..3006)
                     /locus_tag="VF276_04310"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEX6606118.1"
                     /translation="MRDLLDHHANAIEYVLHAHDINGRVAGGALSPRLVHFHLRLPST
                     VRTSRIAQLLPQIAEALQVPAVRLAPDPETGEPVLEVPRPDPVAVRLLPLAKKVAEIV
                     PPCTATLGLDTGGTPLLLRLDAPEVCPTLVIGAPAAGKTHLLQTMAVSLALHNSPDTL
                     RMLLMDVSGPARWGRKREQSAWDALAPLPHLVTEPVREPQEAYIRLRWVGRLMKERAD
                     LLTEGESIEGASLVMLVDGLEELAAGPHATESLDLLERIARDGRELGVYLVASSRGSS
                     LVDQLSWGARLVGRCRDAAAARAASSMQGSGAEGLLGGGDFLALLGGEAVRFQAATLG
                     PEEVARTVALLQQVADAEEMSAEMELRAGRPNPAHKGSQMLQRRSGAGPSLLR"
     gene            complement(3067..3756)
                     /locus_tag="VF276_04315"
     CDS             complement(3067..3756)
                     /locus_tag="VF276_04315"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HEX6606119.1"
                     /translation="MPDPRWTAPRGAADALAPGAAPAWGAFDRAPGAGVTIREASARI
                     DGMRVLGSEPVAPGAAFRRNSKNAGVYVTEARRRVAARATVRVPVVGEIAAGRYDATV
                     AYHDYLEYEHGVEVDRALVRDGSGVFALRVRGTSMTHVGIDPGDLVVIHPQDHADNGD
                     FVVARLTDSDDPEGYVTLKRFYRKQDHIFLQSATAAQEPIRLYPNARSRGADRDRVKV
                     QGRVIVVIKSS"
     gene            complement(3781..4449)
                     /gene="lexA"
                     /locus_tag="VF276_04320"
     CDS             complement(3781..4449)
                     /gene="lexA"
                     /locus_tag="VF276_04320"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015408062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX6606120.1"
                     /translation="MAKNISERQTKILEFIRKFIDDHGFPPSVRDIQEGLQPRISSTS
                     VVDYNLKALEERGLIRRGKGISRAIELVENGNGHRNNMTVFRIPVAPTPIAAGSPIPV
                     LQDVRASTEDTLDVSQMLLGRHAAHPESLYALRVKGRSMIEDLINDGDVVIVLAQETA
                     EVGQTVVAWLRNEQEATLKRFYLESDGRVRLQPANETMDPIFTEADNLEIKGRVVGVL
                     RHVD"
BASE COUNT          814 a         1688 c         1588 g          763 t
ORIGIN      
        1 ccggcccggc tgattcgtct gctgggctgt attgtgctgg ggctggcgtt gctgaccggg
       61 tgcgactttt tcgcgccggc gcccaatacc acccccgtcc ccagcccccc gcccgcgaca
      121 accgcaggat cgaccgcgcc cctcgccacc accggcccgg cgtccaccgt cgcgcccacg
      181 ccgactacgc cgcccaatgt gccgcgcggc ggcacgctca ccgtgcgtat cccgcacgac
      241 gtgaccacgc tcaatccgct gttcgtggat aaggggccgg gcaaccagga tgcggcggcg
      301 gcgcaagtga ccgggctgat cttcagcggc ctgacccgcc tggacgacca gttgcgcccg
      361 gtgcccgacc tggccgaaag ctgggacgtg gcccccgacg gcttgagcct gaccttccac
      421 ctgcgtcccg gcgtcaagtg gcaagacggc agcccggtca cgtcggcgga cgtgatctgg
      481 acctacaaca cctggctcaa cctcaccggc acgaccacgt tgcaatacca tctgcgcgac
      541 gccgtgttgc agatccagcc cgccgacccg cccgagtcga ccgtgcgctt ctggctcaag
      601 aagccctacg cgccgatcct ggccgacctg gccgccccca tcctgcccaa gcacctgctg
      661 ggcaatgtgc cgctcgatca gcttgcggcc agcgatttca atttcaagcc ggtgggcacg
      721 ggaccatacg agttcagcga tcatagggaa ggcgaaaacg tcatcctgac ggccaacccg
      781 aactattacg gcgggccgcc caacatcagc cgggtcgcct tcctggtagc gcccgacccg
      841 caggtggccg tgaacgcgct aagccagggc acgctctcgc tggccgaggt gccgcaggcg
      901 gcgtgggagg agtatgcgcg gcggcccggc atccagagcc tgttcaccct ggcgcgctat
      961 gccgatccca gctactattt cgtggccttc aacgtgcgcg cgggccacgc gctgagcgac
     1021 ccgcgcttgc gccaggcgtg ggcgctggcg ctcaacaagg aaccactggt gcgcgaggcc
     1081 accggcggcg cgggcctacc tatctggagc gacatcaacc cggcgtcgtg ggccttcgcg
     1141 cccgacacgc cccggctcaa caacgactcg gcacgcgccc gccagttgct gactgaggcc
     1201 gggtggaccg acacgaacaa cgacggcatc gtggataagg gcggggagcc gctcaaaatc
     1261 agcctctatg tgcgggccga cgaccccgcg cgccgcaagg ccgccgaact gatggggccg
     1321 ccgctcgccg ccgtgggcat cagcaccacg gtcgtcccgg tggacttcga gtcggtgatc
     1381 aacgccaaga tcgatccgct gcacgagccg gcgttcgact tccacgccat ggtcatgggc
     1441 tgggacaacc agtcgcccga cccggacgac tacccgctgt tccattccag ccagatccgc
     1501 agcaagagca acccgaacgg cctgaactac atcggctaca actcgccgga gtacgacgaa
     1561 ctgagccgca aagcgcgcag cgagtacgac ccggtgaagc gcgcggcgct ctacgcgcag
     1621 atccagggcc tgctggccca ggacctgccc tactacttcc tctgggccga ccagcactat
     1681 atggtcttga accaccgcgt gcaggggccg atcaatctgg cctcgccgcg ctatctctgg
     1741 aacgtggaga aatggtggat cgagccgtag ccggacgggg gcacggcacg cacaaaaagg
     1801 gcggcccgca ggccgccctt tcgtttgcag tatggcagta ggtcagcgca gcaggctggg
     1861 cccggcgccg gagcgccgtt gcagcatctg cgaccccttg tgcgccgggt tgggccgccc
     1921 ggcccgcaac tccatttccg ccgacatctc ctcggcgtcg gcgacctgtt gcagcagggc
     1981 cacggtgcgg gccacttcct ctgggccgag cgtggcggcc tggaagcgca cggcctcgcc
     2041 gcccagcagc gccaggaagt cgccgccgcc cagcaggccc tcggcgccgc tgccctgcat
     2101 gctcgacgcc gcccgcgctg ccgcggcatc ccggcagcgc cccaccagcc gggcacccca
     2161 actcagttgg tccaccaggc tcgaaccgcg gctcgacgcc accagataga cgcctagttc
     2221 gcggccatcg cgggcaatgc gctccagcag gtcgagcgac tcggtggcgt gcggcccggc
     2281 ggccaactct tccagcccgt cgaccagcat caccagggac gcgccctcga tggattcgcc
     2341 ttcggtgagc aggtccgctc gctctttcat caggcggccc acccagcgca gccggatata
     2401 ggcttcctgg ggttcgcgca ccggctccgt gaccaggtgc ggcaagggcg caagcgcgtc
     2461 ccaggcactt tgctcgcgtt tgcgccccca gcgcgcaggc ccgctgacat ccatgagcag
     2521 catgcgcagc gtatcggggc tgttgtgcag agccagcgag acggccatgg tctgcaacag
     2581 gtgcgtcttc ccggcggcgg gcgccccgat cacgagcgtg gggcagacct ccggcgcgtc
     2641 gaggcgcaac agcaggggcg tgccgccggt gtcgagaccc agggttgccg tgcagggcgg
     2701 cacgatttcg gcgacctttt tggcgagcgg caacaggcgc acggccaccg ggtcggggcg
     2761 cgggacttcg agcaccggtt ccccggtttc ggggtcgggc gccagacgca ccgcgggaac
     2821 ctgcagggct tcggcgatct gcggcaacag ttgcgcgatc cgcgacgtac gcacggtcga
     2881 cggcaggcgc aggtggaagt ggaccagacg cggcgacagc gccccgcccg ccacccgccc
     2941 attgatgtcg tgggcgtgca gcacgtactc tatggcattg gcatgatggt ctaacaagtc
     3001 gcgcatcgag tcccaggggc atgataataa ggcggcacgg gcgccacgta gcccacgcga
     3061 cctaggttag ctactcttta taacgacaat tacccggccc tgtactttca cccggtcccg
     3121 atctgcaccc cggctgcgag cgttggggta gagccggatc ggttcctgcg ccgccgtggc
     3181 cgactgcaag aagatgtggt cttgcttgcg gtagaagcgc ttcaaggtga cgtagccttc
     3241 cgggtcgtcg gagtcggtca ggcgggcgac gacgaagtca ccgttgtcgg catgatcctg
     3301 gggatggatg acgacgaggt cgccgggatc gatgccgaca tgggtcatgg aggtgccgcg
     3361 cacgcgcagg gcaaagacgc cgctgccgtc gcgcaccagg gcgcgatcga cttccacgcc
     3421 gtgttcgtat tcgaggtagt catggtaggc cacggtggcg tcgtagcggc ccgcggcgat
     3481 ctcgccgacg acgggcacgc gcacggtggc gcgggcggcg acccggcgcc gggcctcggt
     3541 gacatagacg ccggcgttct tgctgttgcg ccggaacgcg gcgccgggcg cgacaggttc
     3601 gctgccgagc acgcgcatgc cgtcgatccg ggcgcttgct tcgcgtatgg tgactccggc
     3661 gccgggggcg cgatcaaacg ccccccaggc gggagccgcg ccgggggcaa gcgcatcggc
     3721 agccccccgc ggcgccgtcc agcgcggatc cggcatggcg gtatccctcc gtcggcgagc
     3781 ttagtctacg tgccgcagaa cgccaaccac gcggcccttg atttcgagat tgtcggcctc
     3841 ggtgaagatc gggtccatcg tctcgttggc cggctgcaag cgcacgcggc catcggactc
     3901 cagatagaac cgcttcagcg tggcttcctg ctcgttgcgc agccaggcca ccaccgtctg
     3961 cccgacttcc gccgtctcct gcgccagcac gatgaccacg tcgccgtcgt tgatcaggtc
     4021 ttcgatcatc gagcggccct tgacgcgcag ggcgtagagg ctctccggat gcgccgcgtg
     4081 gcggccgagc agcatctgcg acacgtcgag cgtatcctcc gtggaggcac gcacgtcttg
     4141 cagcacgggg atgggcgaac ctgcggcgat gggcgtcggc gccacgggga tgcggaatac
     4201 ggtcatgttg ttgcgatggc cgttgccgtt ctccaccagc tcgatggcgc gcgaaatgcc
     4261 cttgccgcgc cggatcagcc cgcgctcttc cagcgccttg aggttgtaat cgaccaccga
     4321 cgtggacgaa atccggggct ggagcccctc ctggatgtcg cgcacgctgg gcggaaagcc
     4381 gtggtcgtcg atgaacttgc ggataaactc caggatcttg gtctggcgct cggatatatt
     4441 ctttgccatg gtctcccctt cgatcagttc gtgtaagccg gctcctggcg gctacacgcg
     4501 cggcacgtcg agtacctgac ccctagctcc atctaccgat aaggcgccaa cgcccgactc
     4561 gcaggtgcgg gccgttgccg ctgatttcca ccagtcgccg ccggcaaaac agaaagtggc
     4621 atctcacaat gacggcaaca cacatacaca gggtacaagg ctactaggct catcatagca
     4681 cagaacaatc gttcgtgtca atagttttgg cggcaaaaac cgaacaaggg tgctattatt
     4741 atagaagccc gcccgcagac ggaacgtggc tccatcggcg ggccgttgcg ttacggcgct
     4801 aaaagcagta gaatgacaaa gaccgcggcg cacggacgct ctgtgctgcc ggt
//
LOCUS       DASUYC010000265         2201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_937619,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000265 DASUYC010000000
VERSION     DASUYC010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937619"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..1286
                     /gene="nuoD"
                     /locus_tag="VF276_04325"
     CDS             18..1286
                     /gene="nuoD"
                     /locus_tag="VF276_04325"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEX6606121.1"
                     /translation="MADERPTWEIQSLEPQAEGDAAVAAVLSEEPHPVQEQELLLNMG
                     PQHPSTHGVLRVLLELDGEIVRKAIPDLGFLHRGIEKIGEYRQYNQFVPWTDRMDYVA
                     AFTSNLAWVQAVEGLMGLEIPPRAKFLRVIFSELQRLASHLLWLGTHVMDLGAVTVFL
                     FAMRERELVLDLLEMQTGARITLNAQRVGGFPKDIPPGFLEKLNDFLRVMPRHIHEYD
                     RLLAENPIFLRRVKGIGVISAAEAVQWGLTGPSLRGSGVAYDIRKARPYAAYDQIDFE
                     IPVGSHGDVYDRYLVRMEEMRQSLKIIRQAADMLPDGPVMTDDIRVALPGKEALMTSI
                     EAMQRHFILVIYGLRPPVGEYYSAVEAPKGELGWYIISHGESNAYRARCRPASFTNLQ
                     GLPIMVEGAQIADVIAAIGSIDIVVGEVDR"
     gene            1286..1945
                     /locus_tag="VF276_04330"
     CDS             1286..1945
                     /locus_tag="VF276_04330"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX6606122.1"
                     /translation="MADTAVRIIPPDQVQEILTRYRDVDPMEAMIPVLQEIQTRFGFV
                     TQAVVDQMAEVLGISETEIYGVVTFYSFFRFSPAGGRTLITCEGTSCYVRGAARLREA
                     IEERLHITAEGTTADNRLSFLPMSVCIGACDLGPLVEVEGHYYSHVTPEKLNAIIDEW
                     VDKPLGEVAHGHDEGATGSGMGPYGYGPPAAALGSALPEQGQPPPGPPRTGQSTRGIR
                     D"
BASE COUNT          404 a          709 c          729 g          359 t
ORIGIN      
        1 agggagaggt atgtcaaatg gctgatgaga gacctacctg ggaaatccag tcgctcgaac
       61 cgcaggccga gggcgatgcg gcggtggcgg ccgtcctgtc cgaagagccg caccccgtgc
      121 aggagcaaga actcctgctg aacatgggtc cgcaacaccc cagcacccac ggcgtgttgc
      181 gggtcttgct ggagctcgac ggcgagatcg tccgcaaggc gatccccgac ctgggcttcc
      241 tgcaccgcgg catcgagaag atcggggagt atcgccagta taaccagttc gtgccctgga
      301 ccgaccgcat ggactatgtg gccgcgttca ccagcaatct ggcctgggtg caggccgtcg
      361 aggggctaat gggcctggag atcccgccgc gagccaagtt cctgcgcgtc atcttctccg
      421 aactccagcg cctcgcctcg cacctgctct ggctcggcac ccacgtcatg gacctgggcg
      481 ccgtgaccgt gtttctgttc gccatgcgcg aacgggaatt ggtgctggac ctgctggaga
      541 tgcagacggg cgcgcgcatt accctgaacg cccagcgcgt gggcggcttc cccaaggaca
      601 tcccgcccgg cttcctggag aagctgaacg actttctgcg cgtcatgccc cgccacatcc
      661 acgagtacga ccggttgctg gccgagaacc ccatcttcct gcgccgggtc aagggcatcg
      721 gcgtaatctc cgccgccgag gccgtgcagt ggggcctcac cgggccgagc ctgcgcgggt
      781 cgggggtggc ctacgacatc cgcaaggccc ggccctacgc cgcctacgac cagatcgact
      841 tcgagatccc ggtgggcagc cacggcgacg tgtacgaccg ctatctcgtg cgtatggaag
      901 aaatgcgcca gagcctcaag atcatccgcc aggccgcgga tatgctgccc gacggcccgg
      961 tcatgaccga cgacatccgg gtggccttgc ccggcaagga agcgctgatg acgtccatcg
     1021 aggcgatgca gcgccacttc atcctggtca tctacgggtt gcgcccgccg gtgggcgaat
     1081 attacagtgc ggtggaggca ccgaagggcg aactgggctg gtatatcatc agccacggcg
     1141 agtcgaacgc ataccgcgcg cgctgccgcc cggcatcctt caccaacttg caggggctgc
     1201 cgatcatggt cgaaggcgcg cagatcgccg acgtcattgc cgccatcggc agcatcgaca
     1261 ttgtcgtagg tgaggtggat cgctaatggc cgatactgct gtacgcatca tcccgccgga
     1321 ccaggtgcag gagatcctga cccgctatcg cgatgtcgat ccgatggagg cgatgatccc
     1381 ggtcctgcaa gagatccaga cccgcttcgg cttcgtgacc caggccgtgg tcgaccagat
     1441 ggccgaggtg ctgggcatct ccgagaccga gatctacggc gtggtcacgt tctacagctt
     1501 cttccgcttc agcccggcgg gcgggcgcac gctgatcacc tgcgagggca cgtcgtgcta
     1561 cgtgcgcggg gcggccaggc tgcgcgaggc catcgaggag cgcctgcaca tcacggcgga
     1621 gggcaccacg gccgacaacc ggctgtcgtt cttgccgatg tcggtttgca tcggcgcgtg
     1681 cgacctgggg ccgctggtgg aagtcgaggg ccactattac agccatgtga cccctgaaaa
     1741 gctgaatgcg atcatcgatg agtgggtgga caagccgctg ggcgaggtgg cccacggcca
     1801 cgacgaaggc gcgacgggca gcggcatggg cccctacggg tacgggccgc ccgccgcggc
     1861 gctgggcagc gccctgccgg agcagggcca gcccccgccc ggcccgccgc gcaccggcca
     1921 gagcacgcgc ggcatccgcg actgacgagc tatcagccgt cagctatcag gtagtttaga
     1981 tagagcagat gatgggtatt gccggcatga ggagcggtca gcatgcgcta atcggcgacc
     2041 agtcattatt actgatagct gacggctgac ggctgacggc tgataactag acaaaggacg
     2101 aactatgagc agcgcggcat actatccatt ggacaccaac gtccggagcc agattacgcc
     2161 ggagcagttc gagcagcgcc agcaggcggc gcgcgatctg t
//
LOCUS       DASUYC010000266         5880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_960167,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000266 DASUYC010000000
VERSION     DASUYC010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5880
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960167"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VF276_04335"
     CDS             <1..667
                     /locus_tag="VF276_04335"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX6606123.1"
                     /translation="VDGVAPALVIPDAPQPAETREVQHFLAGKTQNDLVLGFPALRRT
                     DPDYYAMEMMNLILGRLGLYGRLGKSVREDQGLAYYAYSGLEAGLGAGPWAVRAGVNP
                     ANVRRAVASILTEIRRMQDEPVRPQELDAGRRFLTGTLPLRLETNNGLVRQILEIELY
                     GLGWDYIQRYPEIVNSLTVEDVQRVARTYLHPDHFILAIAGPTVPDAAPAEAAPGDAA
                     PPG"
     gene            746..1936
                     /locus_tag="VF276_04340"
     CDS             746..1936
                     /locus_tag="VF276_04340"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX6606124.1"
                     /translation="MSDWLPGGTGPVSRAAGPGAGADPETDIDVLAVGELVVDMVANG
                     SGPLLTATTFTRAPGGIPAHVAAGVARLGRRAALLSLVGDDPFGRYLIGALETYGVDT
                     TYIGVHPRGRTPVAFVSRKDPHAEAPDAGAGSGTVTEAQALFYREGGADQLLSPEWVP
                     AEAVARAQVVLGSGVSLAHALPRAALHAALGHAAQTGAAVAFDVNWQPALWQYPERAR
                     DLYHAVLPQVDILMLSSQELIMLMGGAASTEAGRLDALLRVWVGRLAVGRTRPLLLAL
                     TRGPAGCTYGLWQPGGRGFEVVNLPACEIAVKDRSGAGAAFLAGLLVALLDRLGPQED
                     DAGAPEAGERRLDLRRLTIGEVAEIFDWANASGALATTRRGALAGLPGRSTVLRLIKG
                     RRSG"
     gene            complement(2085..2273)
                     /locus_tag="VF276_04345"
     CDS             complement(2085..2273)
                     /locus_tag="VF276_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606125.1"
                     /translation="MGRLLFGIVLAIIGLMYLAAAWMSVFTSAGFLTGNLFGALLFLV
                     VGVLAAGASVNLLREKRR"
     gene            2478..3365
                     /locus_tag="VF276_04350"
     CDS             2478..3365
                     /locus_tag="VF276_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016362829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6606126.1"
                     /translation="MATRLEEHYIPTNGITLHVMAAGPSEGPLVVLLHGFPEFWYGWR
                     RQIPVLAAAGYRVVVPDQRGYNRSDKPRGLSAYRSNHMAADIVELLDAVGRERAAIVG
                     HDWGALVGWLLALEYPERVARLAILNVPHPKVLEYNLRHNMRQRIRSLYAVFFQLPGL
                     PERLLRAGNWRALAETMRRSACPGSFTDADLRRYRAAWARPGAITAMLNWYRAALRRP
                     AHQPADPRVHVPVLMIWGARDQALGRAMAPQSIALCDDGRLHVFEDATHWVQHDAADR
                     VNALLLDFLAATKQVGAAA"
     gene            complement(3396..4604)
                     /locus_tag="VF276_04355"
     CDS             complement(3396..4604)
                     /locus_tag="VF276_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606127.1"
                     /translation="MASNDYHFFTHWRVRGDIEEVYAILADGASIARWWPAAYRKAHP
                     VDLGDGQEGRNAVYMVARGWLPYTLGFRCRLLESHAPTYLRVEAQGELDGEGVWRLAQ
                     DGPWVDVTYEWTVKATKPLLRYLSFILRPLFAVNHDWVMMKGEESLKVELARRHAHTP
                     AEQARIPAPPGPSPITPLLSILAAGLALGGAVLLYRKLTAPRAGQQDFQFDPDRVAYF
                     ETYGWRAYYDRQWPWLLRLMVGINQEQFHMPLLPALLGAYYVVRASLAWAPVEHDERI
                     VQDYYAKFYRLARRYSGLRFDPERAGYLEMRYNDDHRRLVGNADKTPFIDTMDQLHAT
                     LFGIAPEQARESAEFRVLAANTVDKITGKTSTDVEGDWQRVEEYLRQCYRSIDRVLAA
                     RQAAPETVSA"
     gene            complement(4644..5255)
                     /locus_tag="VF276_04360"
     CDS             complement(4644..5255)
                     /locus_tag="VF276_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020470626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide cyclase"
                     /protein_id="HEX6606128.1"
                     /translation="MASNDYHFITHWRVEGDIKEVADILGNGPDLVRWWPSVYLNAEE
                     LEPGDAAGVGRVISLYTKGWLPYTLRWQFRVTESRYPHGFTLEAWGDFDGRGIWTLAQ
                     DGPWVDITYDWKIRAEKPLLRYFSFLMKPIFGANHRWAMAQGEESLDLELARRHAATP
                     EERALIPAPPGPTSVAPGAMVVALAAMIAGAGWALRRAQAARE"
     gene            complement(5285..>5880)
                     /locus_tag="VF276_04365"
     CDS             complement(5285..>5880)
                     /locus_tag="VF276_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606129.1"
                     /translation="LLYDATDLPRWWPSVYREVRQIRPNDATGTGSRFTLRAAGWLPY
                     TIRFQFQTIEARRPSRLALQAQGDLTGEGVFTLVQDGEWANVTYDWRIEADKPILRYF
                     SFILKPLFATNHHWTMARGLESLQRELARRHAAGIAEQARVLPPPARGGGSLRPLLGL
                     AALALAGWAASRTPEARRLLETGRVRRDRWLGPGPGR"
BASE COUNT          886 a         2036 c         2082 g          876 t
ORIGIN      
        1 ggtggacggc gtcgcgcccg ccctggtcat ccccgacgcg ccccagcccg ccgaaacccg
       61 cgaagtgcag catttcctgg ccggcaaaac gcaaaacgat ctggtcctgg gcttcccggc
      121 gctgcgacgg accgatcccg actactacgc gatggagatg atgaacctga tcctggggcg
      181 gctgggtctt tatggccgcc tgggcaagag cgtgcgcgag gaccagggcc tggcctacta
      241 cgcctacagc ggcctggagg cgggcctggg cgcggggccg tgggctgtga gggcgggggt
      301 caacccggcc aacgtccgcc gcgccgtcgc cagtatcctg accgagatcc ggcgcatgca
      361 agacgaaccc gtgcgcccgc aggaattgga cgcgggccgg cggttcctca ccggcaccct
      421 gccgttacgg ctcgaaacga acaacggcct ggtgcgccag attctcgaaa tcgagctata
      481 cgggctgggc tgggattata ttcagcgata cccggagatc gtcaacagcc tgaccgtcga
      541 ggatgtgcag cgcgtggccc gcacctacct ccaccccgac cacttcatcc tggcgattgc
      601 cggccccacc gtgcccgacg ccgcgcccgc ggaggccgcg ccgggcgatg ctgccccgcc
      661 cggctaaacg ccgccctggc gaggggccgg gcgaacaccg gtgcccgccc catccgatcc
      721 cgcgccgcac tggaggagag agcctatgag cgactggttg cctggaggga ccggcccggt
      781 gtcgcgtgcc gcgggccccg gcgcgggcgc tgatcctgaa acggatatcg acgtgctcgc
      841 cgtgggcgaa ctcgtggtcg atatggtcgc caacgggtcg ggcccactgc tgaccgcgac
      901 caccttcacc cgcgcgcccg gcggtatccc ggcccatgtg gccgccgggg tggctcgcct
      961 gggccgccgc gccgcgctgc tcagcctggt cggcgacgat cccttcgggc gctacctgat
     1021 cggcgcgctg gagacctacg gcgtggacac cacctacatt ggcgtgcacc cgcgcgggcg
     1081 cacgccggtg gcctttgtca gccgcaaaga tccccatgcc gaggcacccg acgccggggc
     1141 cggcagcggc accgtgaccg aggcgcaggc gttgttctac cgcgagggcg gggccgacca
     1201 gttgctgagc cccgagtggg tgccggcgga agcggtggcc cgcgcgcagg tggtgctggg
     1261 gtcgggcgtc tcgctggccc atgcgctgcc gcgcgccgcg ctgcacgccg cgctgggcca
     1321 cgcggcgcag acgggggcgg cggtggcctt cgacgtgaac tggcagccgg cgctctggca
     1381 atatcccgag cgcgcccgcg atctctacca cgccgtgctg ccgcaggtcg acatcctgat
     1441 gctcagcagc caggagctga tcatgctcat gggcggcgcg gcctcgaccg aggcggggcg
     1501 gctcgatgcg ttgttgcgtg tctgggtggg ccggctggcg gtggggcgca cccggcctct
     1561 gctgctggcg ctgacgcgcg gcccggcggg ctgcacctac ggcctgtggc agccgggcgg
     1621 gcggggcttc gaggtggtca atctgccggc ctgcgagatc gcggtcaagg accgcagcgg
     1681 ggcgggcgcc gccttcctgg ccgggttgct ggtggcgctg ctcgaccggc tggggccgca
     1741 ggaggatgac gccggcgcgc cggaggcagg cgagcggcgg ctggatctcc gccggttgac
     1801 gattggcgag gtggccgaga ttttcgactg ggccaacgcc tcgggcgcgc tggcgacgac
     1861 gcgccggggc gcgctggcgg gcttgccggg gcgcagcacc gtcctgcgcc tgatcaaggg
     1921 ccggcgctcg ggctagtagc cgggcaccat gctttgtggt gtaggggccg gttgcaaacc
     1981 ggcctgccgc cccgcagaat caatctagcc ggcccgccaa cttcggcggt gtaggggccg
     2041 gtttgcaacc ggcctgccgt ccggcagaat caatgcgcgg acgactaacg ccgcttctcg
     2101 cgcagcaggt tgaccgaggc cccggcggcc agcacaccca cgaccaggaa cagcagcgcg
     2161 ccaaagaggt tgccggtcag gaagcctgcg gatgtgaaca ccgacatcca ggccgccgcc
     2221 aggtacatca gcccgataat agccagcacg atgccaaaca ataaccgtcc catgattcga
     2281 cgtctccctt gcgcgggccg gatggcggcg ggcgcattgt ctctcaggcc caccgtgacg
     2341 ggcagttcca ctccatagcc cgattatagc aaatcacccc gatcttgacc agcacgcgac
     2401 ccggtggccc ctggcacagg tcctgcggag gatgccgggc agggaccgcc ggacggcccg
     2461 gctacggagg gaaaggcatg gcgacacgtc tcgaagaaca ctacatcccg accaacggca
     2521 ttacgctgca cgtgatggcc gccggcccct ccgaggggcc gctggtggtc ttgctgcacg
     2581 gcttcccgga gttctggtac ggctggcggc ggcagatccc cgtgctggcc gccgcgggct
     2641 accgggtggt ggtgcccgac cagcgcggct ataaccgcag cgacaagccg cgcgggctga
     2701 gcgcttatcg cagcaaccac atggccgccg atatcgtcga gttgctcgac gcggtagggc
     2761 gggaacgggc cgcgattgtc ggccacgact ggggcgcgct ggtgggctgg ctgctggcac
     2821 tggaataccc tgagcgggtg gcccgcctgg cgatcctcaa tgtgccccac cccaaggtgc
     2881 tggagtacaa cctgcgccat aacatgcgcc agcgcatccg cagcctctac gccgtgttct
     2941 tccagttgcc gggcctgccc gaacggctgc tgcgagcggg caactggcgc gccctggccg
     3001 agacgatgcg ccggtcggcg tgccccggca gcttcaccga cgccgatctg cgccgctacc
     3061 gggcggcgtg ggcgcgcccc ggcgcgatca ccgccatgct gaactggtac cgcgccgcgc
     3121 tgcgccgccc ggcgcaccag cccgccgatc cccgtgtcca tgtgccggtg ctcatgatct
     3181 ggggcgcgcg cgaccaggcg ctgggccgcg caatggctcc gcagagcatc gccctgtgcg
     3241 acgacgggcg gctgcacgtc ttcgaggacg cgacccactg ggtgcagcac gatgcggcgg
     3301 atcgcgtcaa cgcgctgctg ctggacttcc tcgccgccac aaaacaggta ggtgccgccg
     3361 cttgacgccg ggagccgatg gacaccggct ctcgtctatg cggataccgt ctccggagcg
     3421 gcctggcgtg cggccagcac ccggtcaata gagcggtaac actgccggag atactcttcc
     3481 acgcgctgcc agtcgccctc cacatcggtc gaggtcttgc cggtgatctt gtcgaccgta
     3541 ttagcggcca gcacacgaaa ctcggcggac tcgcgggctt gctccggcgc gatgccgaag
     3601 agggtagcgt ggagctggtc catggtgtcg ataaagggcg ttttgtccgc gttgccgacg
     3661 aggcggcggt ggtcgtcgtt gtagcgcatc tccaggtacc cggcgcgctc cgggtcgaat
     3721 cgcagccctg aatagcgccg ggcgaggcgg taaaacttcg cgtagtagtc ctggacgatc
     3781 cgctcgtcgt gctcgaccgg cgcccaggcg agagacgcgc gcacgacgta ataggcgccg
     3841 agcagcgcgg gcagcagcgg catgtggaac tgctcctggt tgatgccgac catgagccgc
     3901 agcagccacg gccattggcg gtcgtagtac gcgcgccagc cgtatgtctc gaagtacgcg
     3961 acccggtcgg ggtcgaactg aaagtcctgc tgcccggccc gcggcgcagt cagcttgcga
     4021 tagagcagca cggcgccgcc cagggccagc ccggcggcca ggatgctcag cagcggcgtg
     4081 atcggcgacg gccccggcgg cgcggggatg cgcgcctgtt cggcgggcgt gtgcgcgtgg
     4141 cggcgcgcga gttcgacttt caaactctct tctcccttca tcatcaccca gtcgtggttc
     4201 acggcaaaga gcgggcgcag gataaaggac aggtagcgca gcagcggctt ggtggccttc
     4261 accgtccact cataggttac gtccacccag gggccatcct gcgccagccg ccagacaccc
     4321 tcgccgtcga gttcgccctg ggcttcgacg cgcagatagg tgggcgcgtg ggattccagc
     4381 agccggcagc ggaagccgag cgtgtagggc agccagccgc gcgccaccat atagacggcg
     4441 ttacggccct cctgcccgtc gcccaggtcc accgggtgcg ccttgcggta agcggcgggc
     4501 caccagcggg cgatactcgc tccgtcggcc aggatcgcgt agacttcctc gatgtccccg
     4561 cgcacccgcc agtgggtgaa gaagtgatag tcgttgctcg ccattgcgtc tcctctcgcc
     4621 ggggcggggt gctgccgatc cgcttactcg cgcgccgcct gcgcccgacg caaggcccag
     4681 cccgcgccgg cgatcatcgc cgccagggcg accaccatcg cgccgggcgc cacgctggtg
     4741 gggccggggg gcgcagggat cagcgcacgt tcctcggggg tggcggcgtg gcggcgggcc
     4801 agctccaggt ccagactttc ctcgccctgg gccatggccc agcggtggtt cgcgccgaag
     4861 atcggcttca tgaggaacga gaagtagcgc agcagcggct tttcggcgcg gatcttccaa
     4921 tcataggtga tatcgaccca cggcccgtcc tgcgccagcg tccagatacc acgcccgtcg
     4981 aagtcgcccc aggcttcgag cgtgaagccg tgcgggtagc gcgactcggt gacgcggaac
     5041 tgccagcgca gggtgtaggg cagccagccc ttggtgtaca ggctgatcac ccggcccacg
     5101 cccgcggcat cgcccggctc cagctcttcg gcgttgaggt agaccgacgg ccaccagcgc
     5161 accaggtcag gcccgttgcc caggatgtcg gcgacctctt tgatgtcgcc ttcgacgcgc
     5221 cagtgggtaa tgaaatgata gtcgttgctt gccattggat cctccaccgg cccagggcgg
     5281 gccgctaacg accagggccg gggcccaacc agcgatcgcg ccgcacgcga ccggtttcca
     5341 gcaggcgccg ggcctcgggc gtgcggctgg cggcccatcc cgccaacgcc agcgccgcca
     5401 ggcccagcaa tgggcgtaac gatcccccgc cgcgcgccgg gggcggcagg acacgcgcct
     5461 gctccgcgat gccggccgcg tggcggcggg ccagttcgcg ttgcaggctt tccaggcccc
     5521 gcgccatcgt ccagtgatgg ttggtggcga acaggggctt gaggatgaac gaaaaataac
     5581 gcaggatcgg cttatcggcc tcgatacgcc agtcataggt cacgttggcc cactcgccgt
     5641 cctgcaccag ggtaaacacg ccctcgccgg tcaggtcgcc ctgcgcctgc agggccagcc
     5701 ggctggggcg ccgggcttcg atagtttgga actggaagcg gatggtatag ggtagccagc
     5761 ccgccgcgcg cagggtgaac cggctgccgg tgcccgtcgc atcgttcgga cggatctggc
     5821 gcacctcgcg gtagaccgac ggccaccagc ggggcaggtc ggtcgcatcg tagagcaggt
//
LOCUS       DASUYC010000267         3315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1865731, whole genome shotgun sequence.
ACCESSION   DASUYC010000267 DASUYC010000000
VERSION     DASUYC010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1865731"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1429
                     /locus_tag="VF276_04370"
     CDS             <1..1429
                     /locus_tag="VF276_04370"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX6606130.1"
                     /translation="GAGRDMIEMVHLHLSPNGRWLLVFVEQGWARSEVHVRDLHAPGG
                     TFQPLITDLDALFGGEVIDNTFYVQTNWEAPRYRVLAIDLEQPDRANWREVIAERPDV
                     VVESMAIVGKRLVLNESKDVTSSVGVYALDGAPLARPALPPLGSLTGLEGEWDGDDLF
                     LGFESYTVPPTVYRYHLPSGELTTWAQVEAPIDLSRFQVERVWYSSKDGTQVPLFILS
                     RRDLVRNGANPTVLSGYGGFNVSNSPVFNRNVVPWLEQGGIYAVANLRGGAEYGEEWH
                     RAGMLDKKQNVFDDFHAAAEYLIREGYTRPERLGIMGGSNGGLLVGAALTQRPELFQA
                     VVCQVPLLDMIRYHHFRIAKLWIPEYGGAGDPEQFAWLLAYSPYHRVEPGTDYPAVLF
                     TTAESDSRVDPMHARKMAALMQSHDPRRPVLLRVETEAGHGQGKPLSKRLAEQTDIWS
                     FLALQLGLERQDTGAGNGGFDQRLD"
     gene            1686..2621
                     /gene="hisG"
                     /locus_tag="VF276_04375"
     CDS             1686..2621
                     /gene="hisG"
                     /locus_tag="VF276_04375"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX6606131.1"
                     /translation="MSYHKAKEFPTALAAREPTVQVPSDGTLKLAMQRSGRLTDDTLG
                     LLRSVGLSVESYGQRLFADCRNFPLSLLYGRDDDIPEYVASGTVDLGVVGQNLLYEEG
                     VDVAELLPLGFGYCKLVLAAHKDAPSAGAADLQGKRIATSYPRSAARFFAEQGVEVEI
                     VTLSGSVEVAPALGLADAIVELTATGSTLLLHDLRTVEVILESEAVLVANKQALTDPR
                     KRQNIDRLIVRLRSAQDARQYKYVMMNAPRAALPAIQTLTPGLKSPTIVPLNDPEWVA
                     VHTVIKEDEFWDIIEQLRAAGASEILVTSIEKLLL"
     gene            2666..>3315
                     /locus_tag="VF276_04380"
     CDS             2666..>3315
                     /locus_tag="VF276_04380"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX6606132.1"
                     /translation="MLVPIKRLADLDAADRERILRRAHGTFDDVLPIVEGVMDAVAAR
                     GDAALREYTAQFDGAQLGDLRVSEAEFEAARSAPARAFLDALAQAAHNITAFHSKHIG
                     REEPVQPQEGVRLWRVWRPIERVGLYIPGGKAAYPSSVLMNVIPARIAGCREIVVCTP
                     PNRQGQVSPAILVAADLLGVHNVFKLGGSQAIAAMAYGTESVPRVYKLFGAGNRWVT"
BASE COUNT          567 a         1084 c         1136 g          528 t
ORIGIN      
        1 cggtgcgggc cgcgacatga tcgagatggt gcacctgcac ctgtcgccca atggccgctg
       61 gctgctggtc ttcgtcgagc agggctgggc gcggtctgag gtccatgtgc gcgacctgca
      121 tgcccccggc ggcaccttcc agccgctgat caccgatctg gacgcgctgt tcggcggcga
      181 ggtcatcgac aacaccttct acgtgcagac gaactgggaa gcgccgcgct atcgcgtgct
      241 ggctatcgac ctggagcaac ccgatcgcgc aaactggcgc gaagtgatcg ccgagcggcc
      301 cgacgtggtg gtggagagca tggcgatcgt gggcaagcgc ttggtcctga acgagagcaa
      361 ggacgtaacc tcgtccgtgg gcgtctacgc gctagacggg gcgccgctgg cgcggcccgc
      421 gctgccgccg ctcggctcgc tgaccgggct ggaaggcgaa tgggatgggg acgacctgtt
      481 cctgggtttc gagtcctaca ccgtaccgcc caccgtatac cgctaccacc tgcccagcgg
      541 cgaactgacc acctgggcgc aggtggaggc gcctatcgac ctgagccggt tccaggtgga
      601 gcgggtctgg tactcatcca aggacggcac gcaggtcccg ctgttcatcc tcagccggcg
      661 cgatctcgtg cgcaacggcg ccaaccccac cgtgctcagc ggctacggcg gcttcaacgt
      721 gagcaacagc cccgtgttca accgcaacgt agtgccctgg ctggagcagg gcggcatcta
      781 cgccgtggcg aatctgcgcg gcggcgccga gtacggcgaa gagtggcacc gcgccgggat
      841 gctcgacaag aagcagaacg tgttcgacga tttccacgcg gcggcggagt acctgatccg
      901 cgagggctac acgcgcccgg agcggctggg catcatgggc ggcagcaacg gcggcctgct
      961 ggtcggcgcg gcgctgaccc agcggcccga actgttccag gccgtcgtct gccaggtacc
     1021 gctgctcgac atgatccgct accatcactt ccgcatcgcc aagctctgga tcccggagta
     1081 cggcggcgcc ggcgacccgg aacagttcgc gtggctgctg gcctactcac cctaccaccg
     1141 cgtcgagccg ggcaccgact acccggcggt gctgttcacc accgccgagt ccgacagccg
     1201 ggtggacccg atgcacgcgc gcaaaatggc ggcgctgatg cagagccacg acccgcgccg
     1261 cccggtgctg ctgcgtgtcg agacggaggc cgggcacggc cagggtaagc cgctgagcaa
     1321 gcgcctcgcc gagcaaaccg acatctggag cttcctggcg ctgcaactcg gcctggagcg
     1381 acaggatacg ggggccggta acggcggatt cgatcagcga ttggactagc ggtgcgcgag
     1441 tcgttgtgtt ataataggcg ctaagtccgc aggagcatat atcgtggatt ttgtcctgac
     1501 cagccaccat agccggcacc accacgccca ttatcatggg cggcggccct ggcatggcct
     1561 ggtttcggca ccgatacctg cgtagcgcat cgacaaaacc aggatactga ccagaggccg
     1621 cagccgtcgc gctgcggcct ttttgtgtgc ccgcggccgg gggcgcaagc aggaggaaca
     1681 aacgattgag ttatcacaaa gcgaaagagt tcccgacggc gctggccgcc cgcgaaccca
     1741 ccgtgcaggt cccctccgac ggcacgctga agctggcgat gcagcgcagc gggcggctga
     1801 ccgacgacac gctgggtctg ctgcgcagcg tgggcctgtc ggtggagagc tacgggcagc
     1861 gcctcttcgc cgactgccgc aatttcccgt tgtcgctgct ctatgggcgt gacgacgata
     1921 taccggaata tgtcgccagc ggcacggtcg acctgggcgt ggtcgggcag aacctgctct
     1981 acgaggaagg cgtggacgtg gccgagctat tgccgctggg cttcggctac tgcaagctgg
     2041 tgctggccgc gcacaaggac gcgcccagcg ccggcgcggc ggatttgcaa ggcaagcgca
     2101 tcgccacctc ttatccgcga tccgcggcgc gcttttttgc cgaacagggc gtggaggtcg
     2161 agatcgtcac cctcagcggc tcggtcgagg tagctccggc gctggggttg gccgacgcca
     2221 tcgtggaact gaccgccacc ggcagcaccc tgctgctgca tgacctgcgc acggtcgagg
     2281 tcatcctgga atcagaggcg gtgctggtcg ccaacaaaca ggcgctcacg gacccgcgca
     2341 agcggcaaaa catcgaccgc ctgatcgtgc gcttgcgctc ggcgcaggac gcgcggcagt
     2401 acaagtacgt gatgatgaac gccccgcgcg ccgccctgcc cgccatccag acgctgacgc
     2461 cggggctgaa gtcgccgacc atcgtgcccc tgaacgatcc ggagtgggtc gccgtgcata
     2521 ccgtgatcaa ggaagatgag ttctgggaca tcatcgaaca actgcgcgcc gcgggcgcct
     2581 cggagattct ggtcacttcg attgaaaagc tattgctcta gagggatata tcacccgtcg
     2641 cggggagcgt gggaggagaa agccgatgtt agtaccgatt aagcgcctgg cggacctgga
     2701 cgccgccgac cgggagcgca tcttacgccg ggcgcacggc acgtttgacg atgtgctgcc
     2761 cattgtcgag ggtgtaatgg acgccgtggc cgcgcgaggt gatgcggcgt tgcgcgagta
     2821 cacggcgcag ttcgacggcg cgcagcttgg cgacttgcgg gtcagcgagg ccgagtttga
     2881 ggcggcgcgg tcggcgcccg cccgcgcctt cctcgacgcg ctggcccagg ccgcgcacaa
     2941 tattaccgcc ttccacagca agcacatcgg gcgcgaggag ccggtgcagc cgcaggaggg
     3001 cgtgcgcctg tggcgtgtct ggcggcccat cgagcgcgtg ggcctgtata tccccggcgg
     3061 gaaagccgcc tatccgtcgt cggtgctgat gaacgtgatc ccggcacgca tcgccgggtg
     3121 ccgagagatc gtcgtgtgca cgccgcccaa ccgccagggc caggtcagcc cggccatcct
     3181 ggtcgccgcc gatctgctcg gcgttcacaa cgtcttcaag ctaggcggaa gccaggccat
     3241 cgcggccatg gcctatggca ccgagagcgt gccgcgcgtg tacaagctgt tcggtgcggg
     3301 caaccgctgg gtcac
//
LOCUS       DASUYC010000268         2494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1215791, whole genome shotgun sequence.
ACCESSION   DASUYC010000268 DASUYC010000000
VERSION     DASUYC010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215791"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VF276_04385"
     CDS             complement(<1..204)
                     /locus_tag="VF276_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606133.1"
                     /translation="MSIPRPGGRALIYLALVAALGVSLLPLGSITHPTQPIYFAETNH
                     TVSGRFLAAWQGPYSYQESVAING"
     gene            573..1055
                     /locus_tag="VF276_04390"
     CDS             573..1055
                     /locus_tag="VF276_04390"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 family protein"
                     /protein_id="HEX6606134.1"
                     /translation="MSASSTEVWKLAAGLGWLAGMRSASAPAAFSYRVARHPAALRGT
                     PFAPLASSRVRTLLTLSALGEFVVDKLPITPSRTDPGPLAGRVGSGALVGAAAFAAHG
                     RPALIGAAIGGGAAFLSTHTLYRLRVAASDNLGIPNLAAGLLEDVAAVALGRFLARSG
                     "
     gene            complement(1092..1508)
                     /locus_tag="VF276_04395"
     CDS             complement(1092..1508)
                     /locus_tag="VF276_04395"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6606135.1"
                     /translation="MSDQAAREPGVQVVRPHERSRATAQTTGMSREAGVAPDTTGSRN
                     LWVGVTTTPPGTVSGWHHHGDCETAIYIVQGRARFSWGPGGGESAEVGPGDFLDVAPG
                     AVHREECLGDEPVVMVVARACNGVIVVNTDGPESGV"
     gene            complement(1562..>2494)
                     /locus_tag="VF276_04400"
     CDS             complement(1562..>2494)
                     /locus_tag="VF276_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606136.1"
                     /translation="LGGPARALEQRFPARNTAERAVYLGYTPILLALLALWLIRRRGV
                     LFWALTAAFTWSLSLGPILHVLGRANFTPYGATVPLPYLVLSTLVPGFSVMRVPSRFI
                     VLSSLALAVLMGYALAALSARLRPTPQPDDMAPPARRVAFPALSARTLAVYGVVAVLI
                     ALEFVTIPYPMAPPGYHIPFYEQVAQEPGRFAILELPLRPMSDYMAYQTVHGKPIVYG
                     YLSRQPPDPFVENTPAVHYLLNTTPVDALPPAEAATGAATLRAAGIRYVVVHWWAFTD
                     PERKAMEAKLAALFPGVTPTPDPAHQMVIYTLGP"
BASE COUNT          370 a          884 c          873 g          367 t
ORIGIN      
        1 gccgttgatg gcgacgctct cctgatacga atacggcccc tgccaggcgg ccaggaaccg
       61 cccggagacg gtgtgattgg tctcggcgaa gtagatcggc tgggtggggt gggtgatgct
      121 gcccagcggc agcagcgaga cgcccagcgc ggccaccagg gccaggtaga tcaacgcccg
      181 gccccccggc cttgggatcg acacgcccat tcgctcctta cccgtgctgt gtagcgcgcc
      241 cccggcatcc gtcattctga gcggagcgaa gaatctccgt ctcgttggga ccgaagacga
      301 gactcttcgc tacgctcaga gtgacaatcg cgcggtgaca ggctacgccg tgctacatac
      361 aaatgattga tccttaccag tccctagtta ctatacgcat tgccgccccg cgacgcaaga
      421 tccccacgcg tggcctgaac cttgcgagaa ccggcgtcgt ttgcgatcca gcgggccggt
      481 ttgaagccgg cccctacagc gcataatgag cggcgcctgc cccaaccacg ccgggcaggg
      541 ctcgggccac cttattggag gaatctgtgt tcatgtctgc gtcgtctacg gaagtatgga
      601 aactggccgc cgggctgggt tggctcgccg ggatgcgctc ggcgtcggcg ccggcggcgt
      661 tcagctaccg ggtggcgcgc catccggccg cgctgcgggg cacgccgttc gcgccgctgg
      721 catcgtcgcg cgtgcgcacg ctgctgaccc tgagcgcgct gggcgagttt gtcgtggaca
      781 agctgcctat cacccccagt cgcaccgatc ccggtccgct ggccgggcgc gtgggatcgg
      841 gggcgctggt cggagccgcc gccttcgccg cgcacgggcg cccggccctc atcggcgcgg
      901 ccatcggcgg cggggccgct ttcctgtcca cccacacgct ctaccggctg cgcgtggccg
      961 ccagcgacaa cctgggcatc cccaacctcg ccgccgggct gctggaggat gtggcagccg
     1021 tcgcgctggg ccggttcctg gcccgtagcg gatgacggcg cacccggcgc gcgggcaact
     1081 cggagtcgag gctagacgcc ggactccggg ccgtccgtat tgaccacgat cacgccgttg
     1141 cacgcgcggg ccacgaccat caccaccggc tcgtcgccca ggcactcctc gcggtggacc
     1201 gcgccgggcg ccacgtcgag gaaatcgccc ggcccaacct cggcgctttc gccgccgccc
     1261 ggcccccacg agaagcgcgc ccggccctgc acgatataga tcgccgtctc gcaatcgccg
     1321 tggtgatgcc agccgctgac cgtgccgggc ggcgtggtgg tcacacccac ccagaggttg
     1381 cggctgccgg tcgtatcggg ggccacgccc gcctcgcggc tcatgccggt ggtttgcgcg
     1441 gtggcccggc tccgctcatg gggccggacg acctgcacgc ccggctcccg tgccgcctga
     1501 tcgctcatac gatgcctctt ctgctaaaac gtctgcttat ccgccgtccc cggcccgcgc
     1561 gtcatggccc cagcgtgtaa atcaccatct ggtgcgccgg gtcgggcgtc ggggtcacgc
     1621 cggggaagag cgccgccagc ttcgcctcca tcgccttgcg ctcggggtcg gtgaaggccc
     1681 accagtggac gaccacgtag cggatgccgg cggcgcgcaa cgtggccgcg ccggtcgccg
     1741 cctcggcggg cggcagcgca tcgaccggcg tggtattcag cagatagtgg accgcgggcg
     1801 tgttctcgac gaacgggtcg ggcggctggc gcgacaggta gccgtacacg atgggcttgc
     1861 cgtggaccgt ctggtaggcc atgtagtcgg acatgggccg cagcggcagt tccaggatgg
     1921 cgaagcgccc cggctcctgc gccacctgct catagaacgg gatgtgatag cccggcggcg
     1981 ccatcgggta ggggatggtg acgaactcca gcgcgatcag cacggccacc acgccataca
     2041 cggccagggt gcgcgcggac agcgccggga aggcgacccg ccgcgcgggt ggtgccatgt
     2101 catccggctg cggcgttggg cgcaggcgcg cgctcagggc cgccagcgcg tagcccatca
     2161 gcaccgccag cgccaggctg ctcaacacga tgaagcggct gggcacgcgc atcacgctga
     2221 agccgggcac gagcgtgctg agcaccaggt agggcaaggg caccgtggcg ccgtagggcg
     2281 tgaaattcgc ccgccccagc acgtgcagga tcgggccgag cgacagcgac caggtgaacg
     2341 cggcggtcag tgcccagaac agcactccgc gccgccggat cagccacagg gccagcagcg
     2401 ccagcaggat cggcgtatag ccgaggtaca ctgcgcgctc cgccgtgttg cgcgcgggga
     2461 agcgctgttc cagcgccctg gccgggccgc cgag
//
LOCUS       DASUYC010000269         2389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_711253,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000269 DASUYC010000000
VERSION     DASUYC010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711253"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VF276_04405"
     CDS             <1..507
                     /locus_tag="VF276_04405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6606137.1"
                     /translation="DAFLGLPADAGGPHLLLAGGGPQRGALEARVAAAGATARVHFLG
                     SLSHARLVAALSAGDIWVVPSETRSNWREQFGRAAVEAMACENAVVTSDSGELPRVVD
                     GAGCVFPEGDAAALRAILVELIAGPARRCALGRAGRARVLACYTPARAAALYAAAYDM
                     VLGCGDCP"
     gene            558..2177
                     /locus_tag="VF276_04410"
     CDS             558..2177
                     /locus_tag="VF276_04410"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6606138.1"
                     /translation="MADQTHNSSSRPWPANLAWATRFLLLAGLIGAIVWSVQATRSGQ
                     AWRAGAQLLQAGHYGAAVTALEDAINLAPPGGGPGYDSAQAHLSLSYAYLARRDFARA
                     AAVLPDARTTPALAPLIALQRARIAAAQGDSTAAQAVLALAPASGGGTAAAGSRRATL
                     WRRGETRFAAGAPDAAEAYRTLAGLPAPPRDPYTLGAWVRLATLAAPTDPAATRQALA
                     AARAALPADPALAVTPDLHLDDLHLTEGLPAAVISDTIPRLEADLQGAAQVADAPPAA
                     QALYWGHALLSLEAWPQAAALLRQAVAADPALADAHAYLGLAQQRQGDAARAEAGYRT
                     AMRLAPDRDLARQLLARLLIAQHRWAEAAPLLDALLARDPGNVVAHLDRAAWAQGQGF
                     DDVAEAEYKAAEQQQIAHEALGTATPDEQALDTSLLLAQFYFVAGPVHHDCSLARDAA
                     QRAVNRRPTAAALDALGWAQHLCGDDATAAATLAHAVAADPQLPSAQYHYGLILKGFG
                     DPAARPALEAAQDLDPGGLWARRALSALASP"
BASE COUNT          292 a          938 c          853 g          306 t
ORIGIN      
        1 gacgccttcc tgggcttgcc cgccgatgcg ggggggccgc acctgctgct ggcgggcggc
       61 gggccgcagc gcggcgcgtt ggaggcgcgg gtcgccgccg ccggggccac ggcgcgggtg
      121 catttcctgg gttcgctgag ccatgcgcgg ctggtggcgg cgctgagcgc gggcgacatc
      181 tgggtggtgc cctcggagac gcgcagcaac tggcgcgagc agtttggccg cgccgcggtc
      241 gaagcgatgg cctgcgagaa cgcggtcgtc acgtcggact ccggcgagtt gccgcgcgtg
      301 gtggacgggg cgggctgtgt cttccccgaa ggcgacgcgg ctgccttacg cgccattctc
      361 gtcgagttga tcgccggccc cgcccggcgg tgcgccctgg gccgggcggg gcgcgcccgc
      421 gtcctggcct gctacacccc ggcccgcgcg gcggcgctct acgcggcggc ctacgacatg
      481 gtgttgggct gcggcgactg tccgtgacgc accgggccgg cacgggcctg catccggcgg
      541 gcgggccgca cacacttatg gcagatcaga cgcacaattc gtcctcccgc ccctggccgg
      601 ccaacctggc ctgggccacc cgcttcctgt tgctggcggg cctcatcggc gcgatagtct
      661 ggtcggtgca ggccacgcgc agcgggcagg cgtggcgcgc gggcgcgcag ttgttgcagg
      721 cgggccatta cggcgccgcc gtcaccgctc tcgaagacgc gatcaacctc gccccaccgg
      781 gcggcggccc cggctacgac agcgcgcagg cccacctctc gctcagctac gcctacctgg
      841 cgcggcgcga tttcgcgcgg gccgcagccg tgctgcccga tgcccgcacc acgcccgctc
      901 tcgccccgct catcgccctc cagcgcgccc gcatcgccgc cgcgcagggc gattccaccg
      961 ccgcgcaggc cgtcctggcc ctggccccgg cgtccggcgg gggcacggcg gcggcgggca
     1021 gccgccgggc cacgctgtgg cggcggggcg agacgcgctt tgccgccggg gcaccggatg
     1081 ccgcggaggc ataccggacg ctggcgggct tgcccgcccc gccgcgcgac ccgtataccc
     1141 tcggcgcctg ggtgcgcctg gcgacgctgg ccgcgcccac cgatcccgcc gccacccgcc
     1201 aggccctggc cgcggcgcgc gccgcgctgc ccgccgaccc cgccctggcc gtgacgcccg
     1261 acctgcacct ggacgacctg catctgaccg aaggcctgcc cgcagccgta atcagcgaca
     1321 ccatcccgcg gctggaagcc gacctgcaag gcgccgcgca ggtggcggac gccccgcccg
     1381 cggcacaggc gctctactgg ggccacgccc tgctgagcct ggaagcgtgg ccgcaggcgg
     1441 cggcgctgct gcgccaggcc gtggccgccg acccggccct ggccgatgct cacgcctacc
     1501 tggggctggc ccagcagcgc cagggcgacg ccgcgcgcgc cgaggccggc tatcgcacgg
     1561 ccatgcgcct ggcgccggac cgcgacctgg cccgccagtt gctggcccgg ctgctgatcg
     1621 cgcagcaccg ctgggcggag gcggcgcccc tgctcgacgc gctgctggcc cgcgatcccg
     1681 gcaacgtggt ggcccatctc gaccgggcgg cctgggccca gggccagggg ttcgacgatg
     1741 tggccgaggc cgagtacaag gccgccgagc agcagcagat cgcccacgag gctctcggca
     1801 ccgccacgcc cgacgagcag gcgctcgata cctcgctctt gctggcgcag ttctatttcg
     1861 tggccgggcc ggtgcaccac gattgctcgc tggcccgcga cgccgcgcaa cgcgcggtga
     1921 accgccgccc caccgccgcg gcgctcgacg cgctgggctg ggcgcagcac ctctgcggcg
     1981 acgacgccac ggcggcggcc acgctggccc atgccgtcgc cgcggacccg caactcccct
     2041 cggcgcagta ccactacggg ctcatcctca agggcttcgg cgaccccgcc gcccgccccg
     2101 ccctcgaagc cgcccaggat ctcgaccccg gcggcctctg ggcgcgccgc gcgctctcgg
     2161 ccctcgcgag tccgtagcag ctgtcagcgg tcagccatca gccgtcaggt gacgtgggat
     2221 caaaggtatg gtgggcggat cgtgcttgga taaaccagac gagactcttc gctgcgctca
     2281 gagtgacaag aaaacggctt acgcaagacg agactcttcg ctgcgctcag agtgacaaga
     2341 aaccatctac tgaccactga ccactgacca ctgaccactc gtctcctga
//
LOCUS       DASUYC010000270         3424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_18341,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000270 DASUYC010000000
VERSION     DASUYC010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18341"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VF276_04415"
     CDS             <1..484
                     /locus_tag="VF276_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606139.1"
                     /translation="SPADAVTPPAEPAAPADAFAYLAAVNGKTRKAASRRNGSAAPEA
                     APAAPVEPPVAEEPATVVYAPAPVEEAPGIDDGMAPVAEAAPAEPSEAAPPARSRRPR
                     TAKAKPAETSPAAEVTEIAADTVAATDEAPDSEAPPASPARGSKKAKPEPEGKQLSLF
                     "
     gene            610..1305
                     /locus_tag="VF276_04420"
     CDS             610..1305
                     /locus_tag="VF276_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606140.1"
                     /translation="MNEVVDNPQPAATREYRLAPEARRTTLVLMLGVLAIAVFAIWSF
                     VALLSGGLQGPEWVSALLMLAILGLSPLVAWGLLAEYNATILTDAAGLTYRTIGGINL
                     TYPWPQVQGLAPAPLGLFTFGDKVRAAGRNAAQSTLAIEREPVTGKDVEIPPAVPEGG
                     AAEALPESEPPRQIVVDPPAPERIANPLARLLYQQSYAGGLPLPMGLQDRPALIDEIA
                     AHRTAATTSQLSV"
     gene            1385..3094
                     /locus_tag="VF276_04425"
     CDS             1385..3094
                     /locus_tag="VF276_04425"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX6606141.1"
                     /translation="MDKQSEKVRIIPLGGLGEVGKNMTVLEYGDDIIIVDIGVAFPEA
                     DQLGVDLVIPDITYLKGKEHRVRGIFITHGHEDHIGSIPFIYPHIPAPIYTTRLALGL
                     IEVKLREHGLMQMADLREVKSGDKVDAGNFIVEFFHVCHSIPDACGLGIHTPEGIIIH
                     TGDFKFDQTPVDGYQTDFATLTRLGSEGVLCLLSDCVHVETPGFTPSEREVTRALDTL
                     FREAPGRIIISTFASLISRVQQIMDLAYYHGRKVALAGRSLDNNVAMAQELGYLQAPP
                     ETLIPIGDVRNLPDDRVVLITTGAQGEPMAALSRIANGDNRYISIVPGDTVILSATPI
                     PGNETSVNRIINSLFTQGADVVYPPQARVHVSGHASQEELKLMINLTRPRFVAPIHGE
                     RRHQVHYARLAEQMGVPRENIFLMDNGSVLEVDEHSAELVDRVPAGDVLVDGLSVGEI
                     GQVVLRDRQFLARDGMLVVIVTIDKSSGAVLAGPDLVTRGFVYAREAEDLLERTKLRV
                     RDTLNGHNGNGNGNGNGNGRGDHTHLTEWSFLNRKIKDTVSDYLYEQTHRRPMVLPVV
                     MEL"
     gene            3128..>3424
                     /locus_tag="VF276_04430"
     CDS             3128..>3424
                     /locus_tag="VF276_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606142.1"
                     /translation="MPPTSGTRSSTRADTAPKVATPPAGNGKSRKGGAGAPPAATPAR
                     PKRETVQTTAATSSRGTRSRSSSHPPAKKTTRAKPKPSGPRMSLNIPQPLQREII"
BASE COUNT          639 a         1188 c         1105 g          492 t
ORIGIN      
        1 gtcgcccgcc gatgctgtga cgccgcccgc ggagcccgcc gcgcctgccg atgccttcgc
       61 gtatctggcc gcggtgaacg gtaagacgcg caaagccgcg agccggcgca acggcagcgc
      121 cgcgccggaa gcagcccccg ccgccccggt cgagccgccg gtggcggaag aaccggcgac
      181 ggtcgtttac gcgcccgccc cggtagagga agcgccgggg atcgacgacg gcatggcgcc
      241 cgtggctgag gcggccccgg cggagcccag cgaggccgcg ccgcccgccc gttcgcgccg
      301 cccgcgtacc gccaaagcga agccggcgga aacgtctccc gccgccgaag tgacggagat
      361 cgccgccgat accgtggctg cgacagacga ggcgccggac agcgaagcgc cgcccgcgtc
      421 cccggccagg ggcagcaaga aggcgaagcc ggagccggaa ggcaagcagc tcagcctctt
      481 ctaacgtgcg ccaccggtgc acctgctgcc ggcccgcacg cagggcacgg actctgccat
      541 agcggaaggc acacgtggcg ataaggccaa tcgtgccgct gtttacgtat actagcgtga
      601 gtattgtgga tgaatgaggt tgtagacaac ccacaacctg ccgcgacccg cgaataccgc
      661 ctggcgccgg aagcgcgccg gacgactctc gtcctgatgc tgggcgtgct ggcgattgcc
      721 gtattcgcga tctggagctt cgtcgcgctg ctgagcggcg gcctgcaagg ccccgaatgg
      781 gtcagcgccc tgctgatgct ggcgatcctg ggtttgagcc cgctggtcgc ctggggcctg
      841 ctggccgaat acaacgcaac cattctgaca gatgccgcgg gcctcaccta ccggaccatc
      901 ggggggatca acctgaccta cccctggccg caggtgcaag gactcgcgcc cgccccgctg
      961 gggctgttta catttggtga caaagtccgt gccgccgggc gaaatgcggc tcaatcaact
     1021 ttagcaatag aaagagaacc agttacagga aaggatgtag agataccacc ggcggtgccg
     1081 gagggcgggg ccgccgaggc cctgccggag agcgaacccc cccggcagat tgtagtcgat
     1141 cctcccgcgc ccgagcggat agccaatccg ctggcgcgcc tgctgtatca gcagtcatat
     1201 gccggcgggc tgcccctacc aatggggttg caggaccgcc cggcgctgat cgacgagatc
     1261 gccgcccacc gcaccgccgc cacaacgtca caactctcgg tctgagatcg gaccccgcgc
     1321 gggtccggcc ggcaccgagt gccgtatctc tccccagcat cctggctgga ggaggagttt
     1381 tagtttggac aagcaatccg aaaaagtgcg tatcatcccc ctcggaggtt tgggggaggt
     1441 aggcaaaaac atgaccgtcc tcgaatacgg ggacgacatc atcatcgtcg acatcggcgt
     1501 cgccttccca gaagccgatc agctaggggt agacctggtc atccccgata ttacctatct
     1561 caagggcaaa gagcaccgcg tgcggggcat cttcatcacc catgggcatg aggaccatat
     1621 cgggtcgatt ccgttcatct acccgcacat ccctgcgccg atctacacca cgcggctggc
     1681 cctcggcctg atcgaagtca agctgcgcga gcacgggctg atgcagatgg ccgacctgcg
     1741 cgaggtgaag tcgggcgata aagtggacgc gggcaacttc atcgtggagt tcttccacgt
     1801 ctgccacagc atccccgacg cctgcggcct gggcatccac acgcccgaag gcatcatcat
     1861 ccacaccggc gacttcaagt tcgaccagac gccggtggac ggctatcaga ccgactttgc
     1921 cacgctgacc cggctgggca gcgagggcgt gctctgcttg ctctccgact gcgtgcatgt
     1981 cgagacgcct ggcttcacgc cttccgagcg cgaagtgacc cgcgcattgg atacgttgtt
     2041 ccgcgaggcg ccgggccgga tcatcatctc gaccttcgcc tcgctgatct cgcgcgtgca
     2101 gcagatcatg gacctggcct actatcatgg ccgcaaagtc gcgctggccg ggcgcagcct
     2161 ggacaacaac gtcgccatgg ctcaggagct gggctacttg caggcgccgc ccgagacgct
     2221 gatccccatc ggcgatgtgc gcaacctgcc cgacgaccgc gtggtcctga tcaccaccgg
     2281 cgcgcagggc gagccgatgg ccgcgctcag ccgcatcgcc aacggcgaca accgctacat
     2341 cagcattgtg ccgggcgaca cggtgatcct gtcggccacc ccgattccgg gcaacgagac
     2401 gagcgtgaac cgcatcatca acagcctgtt cacgcagggc gccgacgtgg tctatccgcc
     2461 gcaggcgcgg gtccacgtgt cgggccacgc cagccaggaa gagctgaagc tgatgatcaa
     2521 cctgacccgg ccccgcttcg tggccccgat ccacggcgag cgccgccacc aggtccacta
     2581 tgcgcggctg gccgagcaga tgggcgtgcc gcgcgagaac atcttcctga tggacaacgg
     2641 cagcgtgctc gaagtcgacg aacactcggc agagctcgtg gaccgcgtgc ccgccggcga
     2701 tgtgctggtg gacggcctga gcgtgggcga gatcggccag gtcgtgctgc gcgaccggca
     2761 gttcctggcc cgcgacggca tgctggtggt catcgtgacc atcgacaaga gcagcggcgc
     2821 ggtgctggcc ggccccgacc tggtgacgcg gggctttgtc tatgcccgcg aggcggaaga
     2881 cctgctggag cgcaccaagc tgcgcgtgcg cgacaccctg aacggccaca acggcaatgg
     2941 gaacggcaac ggcaacggca atggccgggg cgaccacacc catctgaccg agtggagttt
     3001 cttgaatcgc aagatcaagg acaccgtgag cgattatctg tatgagcaaa cccaccgccg
     3061 gcccatggtg ttgccggtgg tgatggaact ctaaccggcg gatcatccgc cctgaaaggc
     3121 ggcacccatg cctcccactt ccggtacgcg ttcctcgacc cgcgccgaca cggcgcccaa
     3181 ggtcgcgact ccgccagccg gcaacggcaa gagccggaaa gggggggccg gggcgccgcc
     3241 cgccgccacg cccgcccgcc ccaagcggga aacggtgcaa accacggcgg ccacaagcag
     3301 ccgcggcacg cggtcgcgct cgtccagcca cccgcccgcc aagaagacca cgcgcgccaa
     3361 gcccaaacca agcgggccgc ggatgagcct gaacatcccc cagccgctgc aacgcgagat
     3421 catc
//
LOCUS       DASUYC010000271         2220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1663307, whole genome shotgun sequence.
ACCESSION   DASUYC010000271 DASUYC010000000
VERSION     DASUYC010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1663307"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="VF276_04435"
     CDS             complement(<1..1026)
                     /locus_tag="VF276_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606143.1"
                     /translation="MNIADVLSGAAGLEGVQWALCGPAMRKVLRGQLAALLPPGQLGV
                     CRLDRAKFKPGRKLTGYYNVDLRAPGAASDTRPIAVTWTPPGSKDLGPPSPELLEMQA
                     EAVERGAAAPFQQLVTADPRCGLRVQVSPLDTDFPQLVRLSDPRHVQAMIAAVYAQEN
                     GAAPPLPARFVITPIRYRPGQRHVLRYDPAGDDGQIDPAGTLFAKLYEGQAGAGAVRV
                     AGWVADQLDAARRGVRGLRPKAYIPADAVVLYPRVVGTPLSGQLQHPDARTAMYLHNA
                     GAALRALHAAPVPALAGLEAHDFAKEIRAIARAAEHVTALLPAVGASIGAMLDRAQEL
                     HARLDPQP"
     gene            complement(1066..>2220)
                     /locus_tag="VF276_04440"
     CDS             complement(1066..>2220)
                     /locus_tag="VF276_04440"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX6606144.1"
                     /translation="ASAPAPFGSAEWSGYLPGLRLALRTQPPDVDLPILPQLVDPAQA
                     RALLEGAIRAGSSRYVDITIESCTPQVMRYKPGSRCTILYHLSYPPELAAGHDWPAFV
                     VAKTYQGKKGQTAYDGMRALWDSPLGQGDVVTVAEPLAFVPDLGLLIQGPIPGDRTLK
                     DLIRAALRTGAPEVRDELHAYLRRTAAGLAALHQSGVQAGKVRDYGDALGELHEMIAE
                     LAPLVPDLPGAATPLLDRLTALAATYPPDAEGPAHGSFRPAQVLLHEGRVGFIDFDSF
                     CRSEPARDLALFLSNIISVGLNTGADEDDDEPVDVAASLDYLAQLEGLAEVFLAQYAA
                     LAPISRPRLALWTAAMIFIQVLEYWAKVKPARLKNMIFLLERRLRADGLV"
BASE COUNT          316 a          774 c          826 g          304 t
ORIGIN      
        1 gggttgcggg tccaggcggg cgtgcaactc ctgggcgcgg tcgagcatgg ccccgatgct
       61 cgcccccacg gcgggcaaga gggccgtcac gtgctcggcg gcgcgggcga tggcgcggat
      121 ctccttggcg aaatcgtgtg cctccagccc ggccagggcc gggaccggcg cggcgtgcag
      181 cgcgcgcaag gccgccccgg cgttgtgcag atacatggca gtgcgcgcgt cggggtgctg
      241 gagttgcccg gagagcggcg tgcccacgac ccgcgggtag agcaccacgg cgtcggcggg
      301 gatataggct ttgggccgca agccgcgcac gccccggcgc gcggcgtcaa gctggtcggc
      361 cacccagccg gccacgcgca cggcgccggc cccggcctgg ccctcgtaca gcttggcgaa
      421 cagcgtgccc gccgggtcga tctgcccgtc gtcgccggcg ggatcgtagc gcagcacatg
      481 gcgctggccg ggccggtagc ggatgggcgt gatcacgaag cgggcgggca gcggcggggc
      541 ggcgccgttc tcctgagcat acacggcggc gatcatcgcc tgcacatggc gcgggtcgga
      601 caggcgcacc agttgcggga agtcggtgtc gagcggcgac acctggacgc gcagaccgca
      661 gcggggatcg gcggtgacga gttgctggaa gggcgccgcc gcgccgcgct ccacggcctc
      721 ggcctgcatc tccagcagct ccggcgacgg cggccccagg tccttgctgc ccggcggggt
      781 ccaggtgacc gcgatggggc gcgtgtcgga cgcggcgccg ggggcgcgca ggtctacatt
      841 ataatagccg gtcagcttgc ggcccggttt gaacttggcc cggtcgagcc ggcagacgcc
      901 cagttgcccc ggcggcagca aggcggccag ttgcccgcgc agcaccttgc gcatggccgg
      961 cccgcagagc gcccattgca caccctccag cccggcggcc cccgataaca catccgcgat
     1021 gttcatgcct gcctccctac cctccagcgc gccacacagg gcgcctcaca ccaacccgtc
     1081 cgcgcgcagc cggcgctcca gcagaaagat catgttcttc aagcgcgccg gtttcacttt
     1141 ggcccagtac tccagcacct ggatgaagat catcgccgcg gtccagagcg ccaggcgggg
     1201 ccgggaaatc ggcgccaggg cggcgtactg cgccaggaag acctccgcca gcccctccag
     1261 ttgggccagg tagtccaggc tggcggcgac atcgaccggt tcgtcgtcgt cttcgtccgc
     1321 cccggtattg aggccgaccg agatgatatt cgagaggaac agcgccaggt cgcgcgcggg
     1381 ttccgagcgg cagaagctgt cgaagtcgat gaagccgacc cgcccttcgt gcagcagcac
     1441 ctgggccggg cggaagctgc cgtgcgccgg cccctcggcg tcggggggat aggtggcggc
     1501 cagcgcggtc aggcggtcga gcagcggggt ggccgcgccg ggcaggtcgg gcacgagggg
     1561 cgccagttcg gcgatcatct catgcagttc gcccagcgcg tcgccgtagt cgcgcacctt
     1621 gcccgcctgc acgcccgatt ggtgcagcgc ggccagcccg gcggcggtcc ggcgcaggta
     1681 ggcgtgtagc tcgtcccgca cctccggcgc gccggtgcgc agggccgcgc ggatcaggtc
     1741 tttgagcgtg cggtcgccgg ggatcggccc ctggatcagc aggcccaggt caggcacgaa
     1801 ggccagcggc tcagctaccg ttaccacgtc gccctgcccc agcggcgagt cccagagggc
     1861 gcgcatccca tcgtaggccg tctgtccctt cttgccctgg taggtcttgg cgacgacgaa
     1921 ggcgggccaa tcgtggcccg cggcaagttc gggcggatag ctcaggtggt acaggatggt
     1981 gcagcggctg cccggcttgt agcgcatcac ctgcggcgtg catgattcta tcgttatgtc
     2041 aacatagcgg gacgacccgg cgcggatggc cccctccagc agcgcccgcg cctgggcggg
     2101 atcgaccagt tgcggcagga tcggcagatc gacatcgggc ggctgcgtgc gcagcgccag
     2161 gcgcaggccg ggcagatagc cggaccactc cgcgctgccg aagggcgccg gtgccgaagc
//
LOCUS       DASUYC010000272         2191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_31170,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000272 DASUYC010000000
VERSION     DASUYC010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31170"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1304
                     /locus_tag="VF276_04445"
     CDS             <1..1304
                     /locus_tag="VF276_04445"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX6606145.1"
                     /translation="VLDKLGLWFRVLAILSALIGVVFAANYIEEKGMPLGDFYSVLAL
                     ATLGTMVVAASQDLTGIFVGIELSSIATYIMTGFARNNRLSNEGALKYFLLGTFATAI
                     LVYGMAWLYGMTGSTNIPLIATQVRTLVASGAAGQGGLLLATLLIVAGLGFKVAAVPF
                     QMWTPDAYQGAPTPVTAIMSVGPKAAGFAAIVRILIEGLGPAWQQWVPVIAILSVLTM
                     TVGNVIALAQRSVKRMLAYSSIAHTGYILVAVASYDPSKVAGANGNQAVASILFYLFA
                     YVFMNMGAFGLVVWLERNGGTEYLKDFRGLSGWAPLPAATMLVLMLSLAGIPPTIGFL
                     GKYYVFLAAINSNLVWLAVIGVLNSALATFYYLRVIWYMYFETPETTLVARPATLVNT
                     GLVFTALATVVLFILGGVFLAMTQLSTPLVLSDLLPALAGR"
     gene            1475..>2191
                     /locus_tag="VF276_04450"
     CDS             1475..>2191
                     /locus_tag="VF276_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HEX6606146.1"
                     /translation="MSTDPVESAKAAGLRYVNTKKPRPGIRREKAGDEFRYLAPDGTV
                     IDDPNKVQRIKALAIPPAWTEVWISTDPRGHIQATGRDAKRRKQYRYHPKWRAVRDET
                     KYGRMMAFGQALPQIREHVAADLARPGLPREKVLATVVRLLETTFIRVGNEEYARANE
                     SYGLTTMHNEHVEVAGSTVRFHFRGKSGVEHDIDVKDRRAAAVIKRCQGLPGQDLFEY
                     KDEHGELQTIDSDDVNEYLRA"
BASE COUNT          358 a          732 c          721 g          380 t
ORIGIN      
        1 tcgtgctgga caaactgggg ctgtggttcc gggtgctggc gatcttgtcg gcgctcatcg
       61 gcgtggtgtt tgccgccaac tacatcgagg aaaagggcat gccgctgggc gacttctact
      121 ccgtgctggc cctcgccacg ctcggcacga tggtggtggc cgcctcgcag gacctgaccg
      181 gcatcttcgt cggcatcgaa ctatcgtcca tcgccaccta tatcatgacc ggctttgccc
      241 gcaacaaccg gctgtcgaac gagggcgcgc tcaagtactt cttgctcggc accttcgcca
      301 ctgcgatcct ggtctacggc atggcctggc tgtacggcat gaccggttcg accaacatcc
      361 ccctgatcgc cacgcaggtg cgcacgctgg tagcgagcgg ggccgcgggc cagggcgggt
      421 tgctgctggc gacgctgctg atcgtggcgg ggctcggctt caaggtggcc gcggtgccct
      481 tccagatgtg gacgcccgac gcctatcagg gcgcgcccac cccggtcacg gcgatcatgt
      541 cggtggggcc gaaggcggcg ggcttcgcgg cgatcgtgcg gattctgatc gaggggctgg
      601 ggccggcctg gcagcaatgg gtgccggtca tcgctatcct ctccgtgctg acgatgactg
      661 tgggcaacgt gatcgccctg gcccagcgca gcgtgaagcg catgctggcc tactccagca
      721 tcgcccacac cggctacatc ctggtggccg tggcctccta cgacccgagc aaggtggccg
      781 gcgccaacgg caaccaggcc gtcgccagca tcctgtttta cctgttcgcc tacgtcttca
      841 tgaatatggg cgccttcggc ctggtggtct ggctcgaacg caacggcggc acggagtatc
      901 tgaaggattt ccgcgggctg tccggttggg cgccgctgcc cgccgccacg atgctggtgc
      961 tgatgctctc cctggccggc atcccgccga ccatcggctt cctgggcaag tactacgtgt
     1021 tcctggcggc gatcaactct aacctggtct ggctggccgt catcggcgtg ctgaacagcg
     1081 cgctggcgac cttctactac ctgcgcgtca tctggtacat gtacttcgag acgccggaga
     1141 cgacactggt ggcgcgaccc gcgacgctgg tcaacaccgg cctggtcttc acggcgctgg
     1201 cgacggtggt gctgttcatc ctgggcggcg tattcctggc catgacccaa ctctcgaccc
     1261 cgctggtgct cagcgacctc ttgcccgccc tggccgggcg ctaggccccg gcctcgcaca
     1321 ctccgcgatc tgcacggcgc cttgcttgcg ggcaaggcgc cgcttttgct agatcgccag
     1381 agcttggcgc cgctccgcac caatgtacct tggcacttca tttgcttcta cagcgcgcat
     1441 gaaccagacc agcacagccc caggcgatcc cgagatgagc acggacccgg tcgagtcggc
     1501 gaaggccgcc gggttgcgct atgtcaacac caagaagccg aggccgggca tccgccggga
     1561 gaaggcgggc gacgagtttc gctatctcgc gcccgacggc acggtcatcg acgacccgaa
     1621 caaggtgcaa cgcatcaagg cgctggcgat cccgcccgcg tggaccgagg tctggatcag
     1681 caccgatccg cgcggccata tccaggccac cgggcgcgac gccaaaaggc gcaagcagta
     1741 tcgctaccac cccaagtggc gcgccgtgcg cgacgagacc aagtatggcc gcatgatggc
     1801 cttcgggcag gcgctgccgc agatccgcga gcacgtggcc gcggacctgg cccggccggg
     1861 gctgccgcgc gagaaggtgc tggcgacggt agtgcgcctg ctcgaaacca cctttatccg
     1921 cgtgggcaac gaggaatacg cgcgagccaa cgagtcctac ggcctgacca cgatgcacaa
     1981 tgagcatgtc gaggtcgcgg gatcgacggt ccgcttccac tttcgtggca agagcggcgt
     2041 ggaacatgac atcgacgtga aggaccggcg cgcggccgcg gtgatcaagc gctgccaggg
     2101 cttgccgggg caggacctgt tcgagtacaa ggacgagcac ggcgagctac agaccatcga
     2161 ctcggacgac gtgaacgagt atctgcgggc c
//
LOCUS       DASUYC010000273         2283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_176620,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000273 DASUYC010000000
VERSION     DASUYC010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176620"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1206
                     /locus_tag="VF276_04455"
     CDS             <1..1206
                     /locus_tag="VF276_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEX6606147.1"
                     /translation="RLRFPVGRDTHGQPVVTDLAKLPHLLIAGATGSGKSVAINALIV
                     SFLMQYTPDELRMLMIDPKRVELSSFNGIPHLLRPVVTEMKHEKEQTKKGPRELTAIE
                     VLKWLLWEMERRYKIFARGSKDADGISRIFRTIEQYHNTARANPAMEPLPYIVLIIDE
                     LADLMLVSPEDVETSLCRLAQLARATGIHLIIATQRPSVDVVTGLIKANFPARIAFAV
                     TSMIDSRVILDTPGAEKLLGRGDALYTASDESKPIRVQGTFLSDKETERIVQFWRKQL
                     PEIHPSPDHAGAPGDGHAGGLALAGQQVQVGANVPMPDWLADGADSGGDDEDAMLQQA
                     IDLVKKHRYASTSMLQRRLRIGYNRAARLVEQMEEMGIVGPADGSRSREVLVPKDADS
                     DTTSAEDGD"
     gene            1239..1463
                     /locus_tag="VF276_04460"
     CDS             1239..1463
                     /locus_tag="VF276_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606148.1"
                     /translation="MREKITNLGDSPALVLSPEILAQMGLSVGDEIEITLADGVLTVR
                     PLPDAERAATIQAAMDDVFARRADALRRLS"
     gene            complement(1625..2101)
                     /locus_tag="VF276_04465"
     CDS             complement(1625..2101)
                     /locus_tag="VF276_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606149.1"
                     /translation="MKLTKLLIIAGLGVLLALIVAACGMAVVPPAAPAPSQAAPGGAA
                     SAAATTRNAYPADPRAVVDAFLADCAANAATDDVVPYVADFRRGDLYAGGAVEFLKIG
                     GRLDSFRTEPALTSTGSTSASVTAVLTVNGSRHVRVLTLISDGGLWHIGYVDTPGE"
BASE COUNT          408 a          803 c          744 g          328 t
ORIGIN      
        1 cggctgcgct tccccgtggg tcgcgatacc cacggccagc ccgtcgtcac cgacctcgcc
       61 aagctgccgc acctgctcat cgccggcgcc accggctccg gtaagagcgt cgccatcaac
      121 gccctgatcg tctccttcct catgcagtat acgcccgacg aactgcgcat gctcatgatc
      181 gaccccaagc gcgtcgaact cagcagcttc aacggcatcc cccacctgct gcgcccggtc
      241 gtgaccgaga tgaagcacga gaaggagcag accaagaagg ggccgcgcga actgacggcc
      301 atcgaggtgc tcaagtggct gctgtgggag atggagcgcc gctacaagat cttcgcccgc
      361 ggcagcaagg acgccgacgg catcagccgc atcttccgca ccatcgagca gtaccacaac
      421 acggcgcgcg ccaacccggc gatggaaccg ctgccctaca tcgtgctcat cattgacgag
      481 ctggccgacc tgatgctggt ctccccggag gacgtggaaa cctcgctctg ccgcctggcc
      541 caactggcgc gcgccaccgg catccacctg atcatcgcca cgcagcggcc ctcggtcgac
      601 gtggtcaccg ggctgatcaa ggccaacttc ccggcccgta tcgccttcgc cgtgacctcg
      661 atgatcgaca gccgcgtcat cctcgacacg cccggcgccg aaaagctgct gggccgcggc
      721 gacgcgctct acaccgcctc cgacgagtcc aagccgatcc gcgtgcaggg caccttcctc
      781 tccgacaagg agaccgagcg catcgtgcag ttctggcgca agcagttgcc cgagatccac
      841 ccgtcgcccg atcacgcggg cgcccccggc gacggccatg ccggggggct ggccctggcc
      901 gggcagcagg tccaggtggg cgccaacgtg cctatgcccg actggctagc cgacggggcc
      961 gacagcggcg gcgacgacga ggacgccatg ctccagcagg ccatcgacct ggtcaagaag
     1021 caccgctacg ccagcaccag catgctccag cgccgcctgc gcatcgggta taaccgcgcg
     1081 gcccgcctgg ttgagcagat ggaggagatg ggcatcgtcg gccccgccga cggcagccgc
     1141 agccgcgaag tcctggtccc caaagatgcg gactcggata cgacctccgc cgaggatggc
     1201 gactaacgtc tcccgtgcgg cgttaaggag agcctattat gcgtgagaag attacgaacc
     1261 tgggcgattc cccggcgctg gtactctcgc cggagatcct ggcccagatg gggctgagcg
     1321 tcggcgacga aatcgagatc acactggccg acggcgtgct gacggtgcgc ccgttgcccg
     1381 acgcagagcg ggcggccaca atccaggccg ccatggacga tgtctttgcc cggcgcgcgg
     1441 atgcgttgcg gcgactgagc taacgggtgg gggccggttt caaaccggcc cgcgcaccac
     1501 gatctatctg taggggccgg tttcaaaccg gcccgcgcca cccagataaa gcaaaaaaga
     1561 ccccggccag cggactggtc ggggtcttcc tatgaaccgg gctagcgcag tacgcgagac
     1621 agcgctactc ccccggcgtg tccacgtagc cgatatgcca gaggccgccg tcggagatca
     1681 gcgtcagcac gcgcacatgg cggctgccgt tgaccgtgag gactgcggtc accgacgcgc
     1741 tggtcgaacc ggtgctcgtc agcgccggtt cggtgcggaa gctgtccaac cggcccccga
     1801 tcttcaggaa ctcgacggcg ccccccgcat agaggtcgcc gcgccggaag tcggcaacat
     1861 agggcaccac gtcgtcggtc gccgcgttgg cggcacagtc ggcgaggaaa gcgtccacga
     1921 cagcgcgcgg gtccgccggg taggcgttgc gcgtggtcgc cgcggcgctc gccgcgccgc
     1981 cgggcgcggc ttgggacggc gccggggccg ccggggggac gaccgccatg ccgcatgcgg
     2041 cgacgatcaa cgccagcagc acgcccaggc cggcgattat cagcagtttc gtcaatttca
     2101 tcctgtttcc tgtatgttcc ttcacggaac ccccaggcaa ggcccagggt ggtggagttc
     2161 gtatggttcc agtatgccat gattcgcgcg gcggcgcgac gggcggatgc cgcgttgtgg
     2221 gcagcgacca gtagcggaac gggctatccg ccaaaaggcg gagacgaatc cggggcacac
     2281 aac
//
LOCUS       DASUYC010000274         4334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_941659,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000274 DASUYC010000000
VERSION     DASUYC010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4334
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941659"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VF276_04470"
     CDS             complement(<1..443)
                     /locus_tag="VF276_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606150.1"
                     /translation="MSRLAPLGLLLGLALILAQLAPHVPGPAPAAPTPTPLPAQVRTL
                     NPKIGVHTRLTDEADPAKIRETLRLVREMGAPWITEYFPWVYIQPNDAAHFDWQHADL
                     VVDAAAAEGLTLIARFDGTPAWARPRDTTWKYLDPDHYADFAAFAG"
     gene            complement(443..1243)
                     /locus_tag="VF276_04475"
     CDS             complement(443..1243)
                     /locus_tag="VF276_04475"
                     /inference="COORDINATES: protein motif:HMM:NF014571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HEX6606151.1"
                     /translation="MAGLRGLGLRAGDHVVVHTALSSFGRVDGGPQALIGALLTVLGP
                     EGTLVAPTMTWRSCYLHTPDGAIPRGVVAYDPATTPCDADMGIVPPALLAWPGARRSP
                     HPLMSVVAVGAQAAALTAEHPIDDPLRPYRVLAGMGGRVLLLGVDHTRNTTVHAAEFL
                     MGLPHLSGPGYALVRDPAAPGGTRTLMIPREPDCSLGFDVLNDLVPRRTRQIGGAVAW
                     LLEGQEVIDRTKALLAGNRGALLCDNPACRTCTRGRHLYSRDSARAAR"
     gene            complement(1442..2917)
                     /gene="proS"
                     /locus_tag="VF276_04480"
     CDS             complement(1442..2917)
                     /gene="proS"
                     /locus_tag="VF276_04480"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX6606152.1"
                     /translation="MAEPQTAADDASSYVEDITDKSDDFSRWYTDVVLRAELADYSPV
                     RGCMVIRPYGYTLWENVRDHLDVRIKRSGCVNAYFPLLIPKSFLEKEAEHVEGFAPQV
                     AWVTKGGEDELAEPYAIRPTSEAIIGYMYAKWIQSYRDLPLLINQWANVMRWEKVTRL
                     FLRTAEFLWQEGHTVHATYDEAEERTLMMLDVYRALCEDDLAIPVVPGRKSEAEKFAG
                     ADRTYSIEALMGDGKALQAGTSHNLGDHFARGFNIKFLDRDGAEKFAYTTSWGLSTRI
                     IGALIMVHGDDQGLILPPKVAPFQVVFVPIWRKETEKADVFASIGRLEHALQSAGVRT
                     KVDWSENTTPGFKFNEWELRGVPLRIEVGPRDVREGKAVLVRRDTRAKEAVSQDMLVE
                     RVQDLLKEIQQGIYDRAVRFRAEHTHWANSYDEFKQLIENRGFIEAWWCGDAACETQV
                     KNDTKATTRCIPLDQSKHPGPGTCLVCGKPASQWVIFARAY"
     gene            complement(3012..3296)
                     /locus_tag="VF276_04485"
     CDS             complement(3012..3296)
                     /locus_tag="VF276_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606153.1"
                     /translation="MSSNADSQGRAWGTAEEAVRRQAGLDDPGNRPAADNATPGPSRA
                     IDSQEPVDPATAVGSPPAREDVESIHDEVAGFVDAGATDAKGGTTPPAEN"
     gene            complement(3545..4294)
                     /locus_tag="VF276_04490"
     CDS             complement(3545..4294)
                     /locus_tag="VF276_04490"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX6606154.1"
                     /translation="MPAIAAVTFDFHNTLVSSDSWLHLEIHTLPAAVLARLAGTGALP
                     PGADAPDVAARAVAEYEAIREEAKASGREVTAAAGVGRVLAALGLDNAVDAAQVDAAM
                     EAAERACLDDAELVPGVRAVLDDLRAAGTRLAVVSSAAYPPFVRWGLERYDLAVYFPV
                     VATSGDTGYYKSDPRLYAWALARLGVAPAHAVHVGDHPRFDVQGAQAAGMRAILFTRG
                     GTHPPDSACRPDATVAHMADLPAVLAELAAG"
BASE COUNT          652 a         1513 c         1497 g          672 t
ORIGIN      
        1 ccggcaaagg cggcgaagtc ggcgtagtgg tcggggtcca ggtacttcca ggtagtgtcc
       61 ctggggcgcg cccaggcggg cgtgccgtcg aagcgggcga tgagcgtcag gccctcggcg
      121 gcggcggcat cgaccaccag gtcggcgtgc tgccagtcga agtgggcggc gtcgttgggc
      181 tggatgtaga cccaggggaa atactcggtg atccagggcg cgcccatctc gcgcaccaga
      241 cgcagggtct cgcggatctt ggccgggtcg gcttcgtcgg tcaggcgggt atggacgccg
      301 atcttggggt tgagcgtgcg cacctgcgcg ggcagcggcg tgggcgtggg cgcggcgggc
      361 gccgggccag gcacgtgcgg ggcgagttgc gccaggatca gcgccagccc cagcagcagg
      421 cccaggggcg cgaggcggga catcaccggg cagcgcgcgc gctgtcgcgg ctgtagaggt
      481 ggcggccccg cgtgcaggtg cggcaggccg ggttgtcgca gagcagcgcg ccgcgattgc
      541 ccgccagcag cgccttcgtc cggtcgatta cctcttgccc ctccagcagc caggccaccg
      601 cgccgccgat ctgccgggtg cgtcggggca ccaggtcgtt cagcacgtcg aagcccaggc
      661 tgcaatcggg ttcgcgcggg atcatcagcg tgcgcgtacc gccgggcgcc gcggggtcgc
      721 gcaccagggc atagcccggc ccgctgaggt gcggcagccc catcaagaac tcggcggcgt
      781 ggaccgtggt gttgcgcgtg tggtccacgc ccagcagcag cacccgcccg cccatcccgg
      841 ccagcacccg gtacgggcgc agggggtcgt cgatggggtg ctccgccgtc agcgcagcgg
      901 cctgcgcgcc cacggccacc accgacatca gcgggtgcgg actgcgccgg gcgcccggcc
      961 aggccagcag cgcgggcggc acgatgccca tgtcggcatc gcagggcgtg gtcgccgggt
     1021 cataagccac cactccgcgc gggatcgccc cgtccggggt atgcaggtag caactgcgcc
     1081 aggtcatggt cggagccacc agcgtgccct ccggccccag cacggtgagc agcgcgccga
     1141 tcagcgcctg cggcccaccg tccacgcgtc cgaagctgga gagcgcggta tggacgacca
     1201 catggtcgcc cgcccgcaac cccaacccgc gcaacccggc caccagcccg gcctcgtcaa
     1261 tgtgcggtat cccgcgtaag cgatcccaga tggtcgtgcc gtccttctag aagtggtcag
     1321 ctatcagccg tcagctatca gctatcaggt aatagcgctc agccaggtgc cagtctccgt
     1381 tatacgcaca gctactccgt aaactcctga cggctgacgg ctgatagctg acggctgacg
     1441 gctagtacgc ccgcgcgaag atgacccact ggctagcggg cttgccgcag accaggcagg
     1501 tgccgggacc ggggtgcttg ctctggtcca gcgggatgca gcgcgtggtg gccttggtgt
     1561 cgttcttcac ctgggtctcg caagcggcgt cgccgcacca ccaggcttcg ataaagccgc
     1621 ggttctcgat cagttgcttg aactcgtcgt agctattggc ccagtgcgtg tgctccgcgc
     1681 ggaagcggac ggcgcggtcg tagatgccct gctggatctc cttcagcaga tcctgcacgc
     1741 gctcgaccag catgtcctgc gagacggcct ccttggcgcg ggtgtcgcgg cgcaccagca
     1801 ccgccttgcc ctcgcgcacg tcgcgcggcc cgacctcgat acgcagcggc acgccgcgca
     1861 actcccactc gttgaacttg aagcccggcg tcgtgttctc gctccaatcg accttggtgc
     1921 gcaccccggc gctttgcagc gcgtgctcca ggcggccaat gctggcgaac acgtcggcct
     1981 tctccgtctc cttgcgccag atcggcacga acacgacctg gaacggggcc accttcggcg
     2041 gcaggatcag gccctggtcg tcgccgtgga ccatgatcag cgcgccgatg atgcgggtcg
     2101 agaggcccca cgaggtggtg taggcgaact tctcggcgcc atcgcggtcg aggaacttga
     2161 tgttgaagcc ccgcgcgaag tggtcgccca ggttgtgcga ggtgcccgct tgcagcgcct
     2221 tgccgtcgcc catcagggcc tcgatggagt aagtacggtc ggcgccagcg aacttctcgg
     2281 cctcgctctt gcggcccggc accaccggga tcgccagatc gtcctcgcac agcgcacgat
     2341 agacatccag catcatcagc gtgcgctcct cggcctcgtc gtaggtggcg tggacggtgt
     2401 ggccctcttg ccagaggaac tccgccgtgc gcaggaacag gcgcgtcacc ttctcccagc
     2461 gcatcacgtt ggcccactgg ttgatcagca gcggcaagtc gcgatacgac tggatccact
     2521 ttgcgtacat gtagccgatg atcgcctccg aggtcgggcg gatcgcatac ggctcggcca
     2581 gttcgtcctc gccgcccttc gtgacccagg ccacctgtgg cgcgaagcct tcgacgtgct
     2641 cggcctcctt ttcgaggaag ctcttgggga tcagcagcgg gaagtaggca ttgacacagc
     2701 ccgagcgctt gatgcgtacg tcgaggtgat cgcgcacatt ctcccagagg gtatagccgt
     2761 agggccggat caccatgcag ccgcgtaccg gcgaatagtc ggccagttcg gcgcgcagga
     2821 ctacatcggt ataccagcgc gaaaaatcat cgctcttatc ggtgatgtcc tcgacatagc
     2881 tcgaagcatc gtccgcggcg gtctgcggtt cggccatcaa tcctactcct taccaagact
     2941 atatcagtaa ataaaagcgg ctcgccgacc cgcgaccggg tggggcgagc cacaaaacgg
     3001 cagcggggcg gtcagttctc ggcgggcggc gtcgtgccgc ctttcgcatc ggtcgcaccg
     3061 gcatcgacaa acccggcgac ctcatcatgg atgctttcca cgtcttcgcg ggcgggcgga
     3121 ctgccgaccg cggtggccgg gtccaccggc tcctggctgt cgatcgcgcg gctggggccg
     3181 ggcgtggcgt tgtccgccgc gggccggttc cccggatcgt ccaacccggc ctgccgccgg
     3241 acggcttcct ccgccgtgcc ccaggcgcgg ccctgagaat ccgcattact gctcataggg
     3301 ttcctttcgt gcggagacgc gcgaattaca cacatcaacc tgggcggcaa gtgcggcaga
     3361 cctgcaccca ctattatacc cgcgtgcgca agccagcgtc aatcgcgctt gcggcgggcc
     3421 taacccccct gccccccttc ccggcgcggg aaggggggaa tcgccggcgc gagggaaatt
     3481 cacgcccctc cgcgcggcgg ggaggggcgg ggggagaggt tggccgggcg ccgggatgcg
     3541 accgtcatcc ggcggcgagt tcggccagca cggcgggcag gtcagccatg tgggcgaccg
     3601 tggcgtcggg gcggcaggcg gagtcgggcg ggtgggtgcc gccgcgggtg aagaggatgg
     3661 cccgcatccc ggcggcctgc gcgccctgca cgtcgaagcg cgggtggtcg cccacatgga
     3721 cggcgtgggc cggggccacc cccaggcggg ccagcgccca ggcatagagc cgggggtcgc
     3781 tcttgtagta gccggtatcc ccgctggtcg ccaccaccgg gaagtagacg gccaggtcgt
     3841 agcgctccag cccccagcgc acaaacggcg ggtaggctgc gctcgacacc acggccagcc
     3901 gggtgcccgc cgcgcgcaag tcgtccaaca ctgcgcgcac gccgggcacc agttccgcgt
     3961 cgtccaggca agcacgctcg gcagcctcca tagcggcgtc tacctgcgcg gcgtccaccg
     4021 cgttgtccag gcccaacgca gccagcactc gccccacgcc cgccgccgcc gtcacctcgc
     4081 gcccgctcgc cttggcctcc tcgcggatcg cctcgtattc ggcgacggcg cgcgccgcca
     4141 cgtcgggcgc atcggcgccg ggcggcaggg cgccggtccc ggccagccgg gccagcacgg
     4201 cggcgggcag cgtgtggatc tccaggtgca gccagctatc actgctcacc agcgtattgt
     4261 gaaagtcaaa cgtcaccgcc gcgattgcgg gcatcattcg ttctccatcc ggctaacccc
     4321 cggcgatgat ctgg
//
LOCUS       DASUYC010000275         2619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_814518,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000275 DASUYC010000000
VERSION     DASUYC010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814518"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="VF276_04495"
     CDS             <1..562
                     /locus_tag="VF276_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606155.1"
                     /translation="PIALATLVQPGAEFVIVVQVAVFANLLLLGRGVAMLRRELAHPE
                     FFAGWPASRRQMLVYHLLPGFGLPLLCGEVVLLGAGAAALGWGAVLPWLVLWPLLMAA
                     NGVIVLLDFERMLRRWPATAETVPNVGPVMLGAVVLVWMITCLGGLLPGLVAMAVVAG
                     AYTLFSGGTGAAAPAAPQDASPGRPA"
     gene            1072..>2619
                     /locus_tag="VF276_04500"
     CDS             1072..>2619
                     /locus_tag="VF276_04500"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEX6606156.1"
                     /translation="MAGLGSRLRSILVLGALLLGAPSGIVSAPGPGTAVQPYIVQAAT
                     ADAAAAAVAQAGGTVRTRLGIINGVAAALDGAALARLGTTPGITLHRDGAVRKADDGH
                     ETDTRGYLLYPAAATDVTGLQQQTVTGPRTACAGGRVTVSTTQQERPPLQGWGVTVAV
                     VDSGLVNASQPGDWADADPATGTLVAEKAGRCFVYRDFLPRSAANGNNGAAAFNSTDQ
                     NGHGTHVISTITDNRATRLAANQPPAPVGVAPQVNLLAARALDSTGAGTYSGVIAAID
                     WVVANKATYNVRVLNLSLYAPVQGPYWADPLNQAVMRAWQAGLVVVAAAGNSGPDAAT
                     ITAPGNVPYVVTVGAVKSGRYTAGGDDELATYSSRGPTESAFVKPDVVVPASRTIAPM
                     PDTSVLALQVVAGRIQEKADPVYQVTKVKDNINYYQLSGTSMAAAEVSGIAALILQAN
                     PGLTNDQVKYRLVSTARAALDVTTGAPAYSVWEQGAGLVDAPQAVFTTTTAAANTGMD
                     IAQDLAGP"
BASE COUNT          393 a          936 c          881 g          409 t
ORIGIN      
        1 gcccatcgcg ctcgccacgc tggtccaacc cggcgccgag ttcgtaatcg tcgtccaggt
       61 cgccgtcttc gccaacctgc tgttgctggg gcgcggggtg gcgatgctac gccgcgaact
      121 ggcccacccc gaattcttcg ccgggtggcc cgcgagccgg cggcagatgc tggtctacca
      181 cctgttgccg ggctttggct tgccgctgct ctgcggcgaa gtggtcttgc tgggcgccgg
      241 ggcggcggcg ctcggttggg gtgcggtgct gccctggttg gtgctctggc cgctgctcat
      301 ggccgcgaac ggcgtcatcg tgctgctgga cttcgagcgc atgctgcgcc gctggccggc
      361 cacggcggaa accgtgccca acgtcggtcc ggtcatgctg ggcgcggtgg tgttggtctg
      421 gatgattacc tgcctgggcg gattgctgcc gggcctggtt gccatggcgg tggtggccgg
      481 cgcgtacacc ctgttctccg gcggcaccgg cgcggcggca cccgcggcgc cccaggacgc
      541 gagtccgggg cggcccgcgt aggcgcggcc cagctttccg atcccgcccg accctgctac
      601 cacccgccat gtacatcaag attttacgga tcggtatttc ttgcgatact gtaaggccgc
      661 ccgcatcacc ccctccgcag cccttctacg ccccgtgccg ccatacccgc cgttattatt
      721 tgcgcttaga taaagcccaa ttttgccgtc aagacagcta tcgtccgtac ccggcgcagc
      781 cccagcccat agctgagacc gcggaccagg cgtgtggaac tggcctctaa tttgctttta
      841 ggttggcggt aaggctaggg catcggcccg gctccgacgg cattattcgg tacttcatcg
      901 cgcagcacac aggacagacc gcgacatcga gatacggcgc tattgctcgg tggcaggcag
      961 gtaccggcgg gaggggagca acaggacacc ggcgtcctcg ctccccagtt ggccccgttc
     1021 ggatccgaat tgtttagccg gttcttcacc gcatccccca ggagcaaacg gatggctggc
     1081 ctcggatctc gcctcagaag catcttagtg ctgggcgcgc tgctgctcgg cgcgccgtcc
     1141 ggtatagtat cggcccccgg ccccggcacc gccgtgcagc cctacatcgt ccaggcggcc
     1201 acggcggacg ccgccgctgc cgcggtggcg caggcgggcg gcaccgtgcg cacgcggctg
     1261 ggcatcatca acggcgtggc tgccgcgctc gatggcgccg ccctggcccg gctcggcacc
     1321 acacccggta tcacgctcca tcgcgacggc gccgtgcgca aagccgacga cggccacgag
     1381 accgacaccc gcggctatct gctctacccg gcggccgcca ccgatgtgac cggcttgcag
     1441 cagcagaccg tcaccggccc gcggacggcc tgcgccggtg gccgggtcac ggtgtccacg
     1501 acgcagcagg aacggccgcc gctccagggc tggggcgtca cggtggccgt ggtcgattcg
     1561 gggctggtta acgcctcaca accgggggat tgggccgatg cggacccggc gacgggcacg
     1621 ctggtggccg aaaaggccgg gcgctgcttc gtctatcggg atttcctgcc gcgcagcgcg
     1681 gccaacggca acaacggcgc cgccgcgttc aacagcaccg accagaacgg gcacggcacg
     1741 cacgtcatct ctaccatcac cgacaaccgg gcgacgcggc tggccgcgaa ccagccgccc
     1801 gcgccggtgg gtgtagcgcc gcaggtgaac ctgctggcgg cccgtgccct ggacagcacc
     1861 ggggccggca cctactccgg cgtgatcgcg gccatcgact gggtcgtcgc gaacaaggcc
     1921 acgtataacg tgcgcgtgct caatctctcg ctctatgcgc cggtccaggg cccgtactgg
     1981 gccgacccgc tgaaccaggc cgtcatgcgc gcctggcagg cagggctggt cgtggtggca
     2041 gcggcaggca acagcggccc cgatgccgca acgattaccg cgccgggcaa cgtgccctac
     2101 gtcgtcacgg tgggcgccgt caagagcgga cgctataccg ccggcggcga cgacgaactg
     2161 gcgacctact ccagccgcgg gcccaccgag tcggccttcg tgaagcccga tgtggtggtg
     2221 cctgcctcac gcaccatcgc gcccatgccc gatacctcgg tcctggcgct gcaggtggtg
     2281 gccggacgca tccaggagaa ggccgacccg gtctaccagg tgacgaaggt caaggataac
     2341 atcaactatt accaactcag cggcacgtcc atggccgccg ccgaggtgag cgggatcgcc
     2401 gcgctgatcc tgcaagccaa tcccggcctg accaacgacc aggtcaaata ccggctggtg
     2461 agcacggcgc gcgcggcgct tgatgtcacg accggcgccc cggcctacag cgtctgggag
     2521 cagggcgcgg gcctggtgga cgccccgcag gccgtgttca ccaccacgac cgccgccgcg
     2581 aacaccggca tggacatcgc ccaggacctg gccggcccg
//
LOCUS       DASUYC010000276         4087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_928131,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000276 DASUYC010000000
VERSION     DASUYC010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_928131"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VF276_04505"
     CDS             complement(<1..594)
                     /locus_tag="VF276_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606157.1"
                     /translation="MTTFPGSPRLLKGAIVGIDPLNPLASVIVFQYNPETLTRTLTPQ
                     MAGGTGSDRAEALRLKGAPVETISVAVEIDATDQLEKADALAVSMGIYPQLSALEMLI
                     YPKSALVIANTVLLAVGTIEIIPPEAPMTLFIWGPKRVLPVRLTTFSIAEEAYDVNLN
                     PIRAKVTLGLQVLSYNDLPLSHPGYALFLAHQVVKETM"
     gene            complement(630..908)
                     /locus_tag="VF276_04510"
     CDS             complement(630..908)
                     /locus_tag="VF276_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606158.1"
                     /translation="MHKHDSSKRDRIRQLLDELNEWEQKYGIRYSGDPRLKSTGESNA
                     RIEDLKAQLAELGAHVHWNGTEYVLDPGTTAPGDPDQAASQPEDDADA"
     gene            complement(933..1772)
                     /locus_tag="VF276_04515"
     CDS             complement(933..1772)
                     /locus_tag="VF276_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606159.1"
                     /translation="MRTHQQKPNHAGPGIIPLSMLQRPSADGPTAAGPVRLHYPAGGG
                     HNFGDVRVHDDRRAGAEPQVAGTKGAETLPEEEGSTGDPEIKRAAGPDAGVPADAGSG
                     SGSGSGGSKATPTLAASKDDYSGDTATESHKNIRFDVTIPSGVTAGDYALVNKLKGSQ
                     KDGSGKAFKVKMYGKTVDFDFSDWQVDSVDEDPVYWSKDGTRWRYNSTATGFYATDDP
                     GPALSSEAGAVYAVKFKMGLYKLADLPEKTTGSISATPLSEYDWQYSVVVDKSGKFSH
                     PAI"
     gene            complement(1797..2642)
                     /locus_tag="VF276_04520"
     CDS             complement(1797..2642)
                     /locus_tag="VF276_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606160.1"
                     /translation="MRTHQRAETHAGPTPIPLPALRRDNPAASAPGGPATLHYTVGAG
                     HDFSRMRVYGDHGAGAWLDDAGQQSRQVKGTESFPEEEGQAGDPELKRLDNGSGTGSG
                     SGSGSGSGSGKAATPTLAASGDTYKDGASESHKSIRFDVTVPSGLTAGDYALVNMVKG
                     SMKNGDGKYFKATMYGKQVDANYADWVVDSRDEDPVYWSTDKARWNFRTTSTGFFATD
                     DPGPALTSETGAIYALKFKMGLYKLADLPTKTTGTISATPLAEYPWQYSVVVDKSGKF
                     SHPAI"
     gene            complement(2677..2961)
                     /locus_tag="VF276_04525"
     CDS             complement(2677..2961)
                     /locus_tag="VF276_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606161.1"
                     /translation="MTPDIDLDIDELVLHGFATGDGEQIGAAIRHELARLVAEEGLPA
                     GLGTGGAIPRLDGGGFQVAAGATTDAIGAQVAHAVYGGLARSAPPQQAKS"
     gene            complement(2958..>4087)
                     /locus_tag="VF276_04530"
     CDS             complement(2958..>4087)
                     /locus_tag="VF276_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6606162.1"
                     /translation="GVTAFLERVRGVVVLATRGRWAGVLRPMRTMEIQKPAPEEQRAL
                     WQAVLGEAAPRLNGHVTRLATEFNLSATAIQATAREALDGADTPDALAARLWDAGRAQ
                     VRPRLDDLAQRIEPAAGWQDLVLPEPALLALHEIAAHVRRRATVYDEWGFATKGARGL
                     GISALFAGASGTGKTMAAEVLAGELDLDLYRIDLSQIVNKYSGETEKNLRRVFDAAED
                     GGAILFFDEADALFGKRSEVKDSHDRYANIEINYLLQRMELYRGLAVLATNMKSALDP
                     AFLRRIRFVINFPFPDATQRAEIWRRIFPAATPTAGLDMARLAQLNIAGGSIRNIALN
                     ATFLAADAGEPVQMRHLLQATRSEYAKVEKPLNATEIGGWL"
BASE COUNT          560 a         1366 c         1385 g          776 t
ORIGIN      
        1 catggtctcc ttgaccacct ggtgcgccag gaagagcgcg taaccgggat ggctcagcgg
       61 caggtcgttg tagctgagca cttgcagccc cagcgtgacc ttggcgcgga tcgggttcag
      121 gttcacgtcg taggcttcct cggcgatact gaacgtcgtg aggcgcaccg gcagcacccg
      181 cttcggcccc cagataaaca gcgtcatggg cgcctcgggc gggatgatct cgatggtgcc
      241 cacggccagc agcacggtgt tggcgatgac cagggcgctc ttggggtaga tgagcatctc
      301 cagcgcggat agttgcgggt agatgcccat gctcaccgcc agggcgtcgg ccttctccaa
      361 ctggtcggtg gcgtcgatct cgacggccac gctgatcgtc tcgaccggcg cgcccttgag
      421 gcgcagcgcc tcggcccggt cggacccggt gccgccggcc atctgcgggg tcagcgtgcg
      481 ggtgagcgtt tccgggttgt actggaacac gatcacgctc gccagcgggt tcagcgggtc
      541 gatgccgaca atcgcgccct taagcaagcg cggggagcct ggaaaggtgg tcatgctcgg
      601 ttctccatca tggagccgca cactgcggct tatgcgtcag cgtcgtcttc cggctgcgaa
      661 gccgcttgat cgggatcgcc gggggccgtc gtgccggggt ccagcacata ttcagtcccg
      721 ttccaatgca cgtgcgcccc caactcggcc agttgggcct tcaagtcctc gatccgggcg
      781 ttggattcgc cggtgctctt gaggcgcggg tcgccggagt accggatgcc gtatttctgt
      841 tcccattcgt tcagctcgtc cagcaattgc cggatccggt cgcgtttgct gctgtcgtgc
      901 ttgtgcatcg ctatgcctcc agggtcctgc ggctagatgg ccgggtggct gaacttgccg
      961 ctcttgtcta cgaccacgga atactgccag tcatactccg acagcggtgt ggcgctgatg
     1021 ctgccggtgg tcttctcggg caggtcggcc agcttataca ggcccatctt gaatttcacc
     1081 gcgtagacgg cgcccgcctc cgagctcagc gccggaccgg gatcgtcggt ggcgtagaag
     1141 ccggtggcgg tcgaattgta tctccagcgc gtgccgtcct tgctccagta gaccgggtct
     1201 tcatcaaccg aatcgacctg ccagtccgag aaatcaaaat cgaccgtctt gccgtacatc
     1261 ttgaccttga acgccttgcc ggatccgtct ttctgcgaac ctttcagctt gttgaccagg
     1321 gcataatcgc ccgccgtcac cccggagggg atggtcacgt cgaagcggat atttttatgc
     1381 gactcggtgg ccgtatcgcc gctatagtcg tccttggacg cggccagggt cggcgtcgcc
     1441 ttggaaccgc ccgaccccga tcctgagccg gatccggcgt ccgcgggcac acccgcatcg
     1501 gggccggcgg cccgtttgat ttctggatct ccagtcgagc cctcttcctc cggcagggtt
     1561 tcggcgccct tggtcccagc aacctgtggc tcggccccgg cacggcggtc gtcgtggacg
     1621 cgtacgtcgc cgaagttgtg gccgccgccc gccgggtagt gcagtctcac cggcccggcg
     1681 gcggtggggc cgtccgcgct ggggcgctgc agcatcgaca gcgggataat ccccggcccc
     1741 gcatgattcg gtttttgttg gtgggtgcgc atcaggtccg ctccttcccg tgccggttag
     1801 atggccgggt ggctgaactt accgctcttg tcgaccacca cggaatactg ccagggatac
     1861 tcggccagcg gcgtggcgct gatggtgccg gtggtcttcg tgggcaggtc agccagcttg
     1921 tacaggccca tcttgaactt caaggcatag atcgcgccgg tctcgctggt gagtgcgggg
     1981 ccggggtcgt cggtggcgaa aaagccggtc gaggtggttc tgaagttcca gcgggccttg
     2041 tcggtgctcc aatacaccgg gtcctcatcc ctggaatcca ccacccagtc ggcataattg
     2101 gcatcgacct gtttgccgta catggtggct ttgaaatact tgccgtcgcc gtttttcatt
     2161 gagcctttca ccatgttgac cagggcgtaa tcgcccgccg tgagcccgga tggaacggtc
     2221 acgtcaaagc ggatactctt atgggattcg ctggcgccgt ccttgtaggt atcgcccgag
     2281 gcagccaggg tcggcgtggc cgctttgccg ctgccggacc ccgaccccga cccggatcct
     2341 gatccggtcc ccgacccgtt gtcgagccgc ttcagttccg ggtcgccggc ctggccctcc
     2401 tcctccggga aggattcggt gcccttcacc tggcgggatt gctgaccggc gtcgtccagc
     2461 caggccccgg cgccgtgatc gccatacacg cgcatgcggc tgaagtcatg gccggcgccc
     2521 acggtgtaat gcagcgtggc cggcccgccc ggcgccgacg cggcgggatt atcgcgccgc
     2581 agcgcgggca acgggatggg cgttggcccg gcgtgggttt cggctcgctg atgggtgcgc
     2641 atgacgtgtg ctcctcgcgg cggtccagcc cgccagtcag gattttgctt gttgcggcgg
     2701 ggcggaacgc gccagcccgc cgtagacggc gtgtgccacc tgggcgccga tggcgtccgt
     2761 ggtcgcgccc gccgccacct gaaacccgcc gccgtccagg cgcgggatcg cgcctccggt
     2821 gcccaggccc gccggcagcc cctcctcggc aaccaggcgg gccagttcgt gccggatcgc
     2881 ggcgccgatc tgctcgccgt cgccggtcgc gaagccgtgc agtaccagtt cgtcgatgtc
     2941 caggtcgatg tcgggcgtca tagccagccc ccgatctcgg tcgcgttcag cggcttctcc
     3001 accttggcgt actcgctgcg cgtggcttgc agcaggtggc gcatctgcac cggctcgccc
     3061 gcgtcggcgg ccaggaaggt cgcgttcagg gcgatattgc ggatgctgcc gccggcgatg
     3121 ttgagttggg ccagccgggc catgtccagg cccgcggtgg gcgtggccgc ggggaagatg
     3181 cgccgccaga tctcggcgcg ctgcgtggca tccgggaacg ggaagttgat cacgaagcgg
     3241 atgcggcgca agaacgccgg atcgagcgcg ctcttcatgt tcgtcgccag caccgccagc
     3301 ccgcgataga gctccatgcg ctgcagcagg tagttgatct cgatattggc gtagcggtcg
     3361 tggctgtcct tgacctcgct gcgcttgccg aagagggcgt cggcctcgtc gaagaagagg
     3421 atcgctccgc cgtcctccgc ggcgtcgaag acgcgccgca gatttttctc ggtctcgccg
     3481 ctgtatttgt tgacgatctg gctcaggtcg atgcggtaga gatccaggtc gagttcgccc
     3541 gccagcacct ccgcggccat cgtcttgccg gtgccgctgg ccccggcgaa cagggcgctg
     3601 atgcccaacc cgcgcgcgcc tttggtggcg aacccccatt catcgtagac ggtggcgcgc
     3661 cggcggacgt gcgcggcgat ctcgtgcaac gccagcagag cgggttcggg cagcaccagg
     3721 tcctgccagc cggccgcagg ctcgatgcgc tgggccaggt cgtccaggcg cgggcgcacc
     3781 tgcgcgcgcc cggcgtccca cagccgcgcg gccagcgcgt cgggcgtgtc tgccccgtcc
     3841 agggcctcgc gggcggtggc ctggatggct gtcgcactca ggttgaactc ggttgccagc
     3901 cgcgtgacgt ggccgttgag gcgcggcgcc gcctcgccca gaacggcctg ccacagggcg
     3961 cgctgttcct ccggggcggg cttctggatc tccatcgtgc gcatgggccg cagcaccccc
     4021 gcccagcgcc cgcgcgtcgc cagcacgacc acgccgcgca cgcgctccag gaaggcagta
     4081 acgccgg
//
LOCUS       DASUYC010000277         2079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1027173, whole genome shotgun sequence.
ACCESSION   DASUYC010000277 DASUYC010000000
VERSION     DASUYC010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027173"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..1373)
                     /locus_tag="VF276_04535"
     CDS             complement(150..1373)
                     /locus_tag="VF276_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606163.1"
                     /translation="MSDNREVKMKSGAARRARLAWGLLALGLALLALGLLLLALHGAL
                     SSNVIGYLLAFNAFLLVGGVVAARRPEHPIGWLFLGVGLSTNLALALNEYALLALVRS
                     PGSLPGGAPAAWLALILQPGAWWLVFNSLLLFPTGRPVSPRWGVVLGLAGAYAVFALV
                     LDAFAFPMLTFDPLPNPYYTSALAPYSGPVNSVYGFGYVLLILITAVLRFRRSGAIER
                     LQLKWLTYSIFLAALLSLVQLLSGVLGIPSGVFANAAILQIFLFAAAIGIAILRYRLY
                     EIDIIIRRTLIYAVLTALLAAVYFGGVTVLQVLLRPLLGANNDLAVVATTLLIAALFF
                     PLRQRVQRFIDRRFYRRKYDAAKTLAAFSHTVRDEVELEQLTGRLVQVVDETMQPRHV
                     SLWLKGVPRAEEVRS"
     gene            complement(1500..>2079)
                     /locus_tag="VF276_04540"
     CDS             complement(1500..>2079)
                     /locus_tag="VF276_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606164.1"
                     /translation="PAAAQPAQPGGQAALLSMIQQFVTAYNAGDTTTLANLFDPSYQG
                     VITNMPPGAPPQATAPTNRATELQNAAPRLVHVTASNCALASAGTVRCDIGLSGGPTA
                     GLPHPYTETAVFTFGNGKIMRLDETLSNTTRSDFAAIFAHLQQPGMPRTGAPGSASDS
                     GMPFLLLALGGLLIAVCGVAVRQVSRGMGTAR"
BASE COUNT          396 a          675 c          717 g          291 t
ORIGIN      
        1 gggctgtgcc agcaggtaaa cccccaccac gacgcacaac ggggtaaaag cccccacgaa
       61 ggccagcgtg agccaggcgc ccagggtcca gcggcccccg gtgcgcaggc tgccaagcct
      121 gggaaggcgg tgaaggcggg agatcacagt catgaacgca cctcttctgc gcggggcaca
      181 cccttcaacc acagcgacac gtgcctgggc tgcatcgtct cgtccaccac ctggaccaac
      241 cgcccggtca gctgctccag ttccacctcg tcgcgcacgg tgtggctgaa ggcagcgagt
      301 gtcttggcgg cgtcgtactt gcggcggtag aagcggcggt caatgaagcg ctgcacccgc
      361 tggcgcaggg ggaagaagag cgccgcgatc aagagcgtgg tcgccaccac cgccaggtcg
      421 ttgttcgctc caagcaaagg gcgcaacagc acctgcagca ccgtcacgcc gccgaagtag
      481 acggcggcca gcagggcggt cagcacggca tagatcagtg tgcgccggat gatgatatcg
      541 atctcgtaga gccggtaacg caggatggcg atgccgatcg ccgcggcgaa caggaagatc
      601 tgcaggatgg cggcattggc gaagacgccc gagggaatac cgagcacgcc gctcaggagc
      661 tggaccaggg acagcagcgc cgccaggaaa atactatagg tgagccattt gagctgaagc
      721 cgctcgatgg ccccggagcg ccggaaacgc aggacggccg tgatcagaat caacaggaca
      781 tagccgaaac cgtagacact gttgaccggc ccgctgtagg gcgccagcgc gctagtgtaa
      841 tacggattcg gcagcgggtc gaacgtgagc atgggaaaag cgaacgcatc caacacgagg
      901 gcaaagaccg cataggcgcc tgccagtccg agcaccacgc cccagcgggg gctcactggg
      961 cgcccggtgg ggaacagcag caggctgttg aacaccagcc accaggcccc gggctggagg
     1021 atcagcgcca gccaggccgc aggcgcgccg cccggcagcg acccagggct gcgcaccagg
     1081 gcgaggagcg cgtattcgtt gagcgccagc gccaggttgg tgctcaagcc cacgccgagg
     1141 aacagccagc cgatcgggtg ctcggggcgc cgcgccgcga ccaccccacc gaccagcaag
     1201 aaggcgttga aggccagcag gtagccaatg acattcgacg agagcgcgcc gtggagcgcc
     1261 agcagcagca ggccgagcgc cagcagcgcc aggcccagcg ccagcaatcc ccaggccaac
     1321 cgcgcccgcc tggctgctcc ggacttcatc ttgacctctc tgttgtcgct cataattgcc
     1381 cgccttgtgg tgagggcatc ccacccgccg cttgtgcata gctgcgcgat ttagcgcgcc
     1441 cggacgagcg ccagtcatgg cgcagctacg cctagcttct cccggcttcg ccgatctctc
     1501 tagcgggcag tacccattcc tcggctaacc tggcggaccg ctacgccgca cactgctatg
     1561 agcagtccac cgagcgccag gaggaggaag ggcataccac tgtcactggc agagccaggt
     1621 gcgcccgtgc ggggcatgcc cggttgctgg aggtgggcaa agatggccgc gaaatcgctc
     1681 ctggtcgtgt tgctcagcgt ctcgtccagg cgcatgatct tgccgttgcc gaaggtgaac
     1741 accgccgtct cggtgtaggg atgcgggagg ccggcggtgg gtccgccgct cagcccgatg
     1801 tcacaccgga cggtgccggc ggacgcaagc gcgcaattgg acgcggtgac atgcaccagg
     1861 cgcggggcgg cgttctgcaa ttccgtggcc cggttggtcg gcgcggttgc ctgcgggggc
     1921 gcgccgggcg gcatgttcgt aatcacgccc tggtacgagg ggtcgaacag gttcgccagg
     1981 gttgttgtgt ccccggcgtt gtaggcggtg acaaactgct ggatcatgct cagcagggcg
     2041 gcctgtccac ccggctgtgc gggttgggcc gccgccggg
//
LOCUS       DASUYC010000278         2265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_38517,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000278 DASUYC010000000
VERSION     DASUYC010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38517"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VF276_04545"
     CDS             complement(<1..476)
                     /locus_tag="VF276_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX6606165.1"
                     /translation="MIAKLGQTRKLLMGILLIPMLLLAGCGTEATPTNVPAAAATNTT
                     APAAATDTPAAAATNTTAPAAATDTAPAATATEATAGTTPTEASSGGSTEPTPAPTPV
                     SQWVPGKYAKTIPAPSKLVKSGILTVGSDASYPPQEYVDASGKPVGMDIDIASELA"
     gene            complement(642..1607)
                     /locus_tag="VF276_04550"
     CDS             complement(642..1607)
                     /locus_tag="VF276_04550"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX6606166.1"
                     /translation="MPQKTPLDDLLEQDGAVIIETWACMLHSLSNTPYGSQPLDELRA
                     NCTACFRAYQALIQDNDQWPLRVFIARIAPLRASQNFGITQVQRAFLIAKQLVRPRLL
                     ARYGDSADMAALLADWRRFELAVDVAVVRFTEDYHARVTADLERQRARWGQLSNQLVE
                     MVTHDELTGLHNYRFFQERIIDEVRRSVRYHRPLSLFMADIDHFKRVNDTFGHQTGNT
                     VLHHVATLIRNKVRNTDIVTRYGGEEFAVLLPETDAGDAMIVAEHVRATVAASRPPRL
                     PHVTVSLGVSTHQRGDLDGARMIGEADHALYQAKQAGRNRVVFFR"
     gene            1940..>2265
                     /locus_tag="VF276_04555"
     CDS             1940..>2265
                     /locus_tag="VF276_04555"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEX6606167.1"
                     /translation="MQDLLSKFRAIRGVGMAAVVGSDGLVIESVHRPELDADALAAVA
                     TNGLLLAEALGRQMERGGALQTIIEYEGGVVLIEPLLRRVLPRGDRRRRAPDRRGLSR
                     ASSTCKR"
BASE COUNT          345 a          701 c          795 g          424 t
ORIGIN      
        1 ctcgccagtt cgctggcgat gtcgatatcc atgccgacgg gcttgccgct ggcgtccaca
       61 tattcttgcg gcggatagct ggcgtcgctg ccgacggtaa gtatcccgct cttgaccagc
      121 ttgctggggg cggggatagt cttggcgtat ttaccgggca cccactgcga gaccggcgtg
      181 ggcgcggggg tcggctcggt gctgccgccg ctgctcgctt cggtcggcgt ggtgcccgcc
      241 gtcgcctccg tggccgtggc ggcgggcgcg gtgtcggtcg cggcagcggg ggccgtcgtg
      301 ttcgtcgcgg cggcagccgg cgtatcagtg gcggcggcgg gcgccgtcgt attggtcgcc
      361 gctgcggcgg gcacgttggt cggcgtcgcc tcggtgccgc agcccgccag caagagcatc
      421 ggtatcagca gaatccccat cagcagtttg cgcgtttgcc caagttttgc gatcattcta
      481 tcgattcctc ctctggatac aacctgccgg gtggcgcagc accccgcggt cgccggctcg
      541 cggaaccggc ggcgggcggc atacgcccga tggaacccgg tcgatcctcg ttcgatcagc
      601 tagagtatac atcaagagcc gcgtccctgc acacaccggc ttcagcggaa gaagaccacc
      661 cggttgcgcc cggcctgctt ggcctggtag agcgcgtggt cggcctcgcc gatcatgcgc
      721 gcgccgtcca ggtcgccgcg ctggtgcgtg ctgacgccca ggctaacagt cacatgcggc
      781 aggcgcggcg ggcggctggc cgcgaccgtc gcgcgcacat gttcggccac gatcatcgcg
      841 tccccggcgt cggtctccgg caagagcacg gcgaactcct cgccgccgta gcgggtgacg
      901 atgtcggtgt tgcggacctt gttgcggatc agcgtggcga catggtgcag cacggtgttg
      961 cccgtctggt ggccgaaggt atcgttgacc cgcttgaagt ggtcgatatc ggccatgaag
     1021 agcgacagcg ggcggtggta acgcacgctg cggcgcacct cgtcgatgat gcgctcctgg
     1081 aagaagcggt agttgtgcag gccggtgagt tcgtcatgcg tgaccatctc caccaactgg
     1141 ttgctgagtt ggccccagcg ggcgcgctgg cgttccagat cggcggtgac gcgggcgtgg
     1201 tagtcctcgg tgaagcgcac cacggccacg tccacggcca actcgaagcg ccgccagtcg
     1261 gccagcaggg cggccatgtc ggcgctgtcg ccatagcggg ccagcagccg gggccggacc
     1321 aactgtttgg cgatcaggaa agcccgctgc acctgggtga tcccgaaatt ctggctggcg
     1381 cgcagcggcg cgatgcgggc gatgaacacc cgcaacggcc attggtcgtt gtcctggatc
     1441 agcgcctggt aagcgcggaa gcacgccgtg cagttggccc gcaactcgtc caggggctgg
     1501 ctcccgtagg gcgtgttgct gagactgtgc agcatacagg cccaggtttc gatgatgacc
     1561 gcgccgtcct gttccagcag gtcgtcaaga ggggttttct gtggcataca tatccatgtt
     1621 tgttggtctg cttcccaaac gggaaaactt gcgttataat tgtccgcaga ttacgtgaca
     1681 taaagccagt cggacgccct gaatagtctg tcaacggcgt tttgtcactc tgagcgcagc
     1741 gaagagtctc gtcttgtgtc ctaacgagac ggagattctt cgctccgctc agaatgacaa
     1801 ttttggtgtg acgggctgct tggaggcccg atttgttgct gatggccggc tgtggagttg
     1861 cgctccgcgg atcacctcgg ccgtgtgtcc cgcgtgacgg cggcgggaca gaaggaggag
     1921 acgctaagtg gcaaccggca tgcaagacct gttaagcaag tttcgtgcga tccgtggcgt
     1981 gggcatggcc gccgtcgtcg gatcggacgg cctggtgatc gaaagtgtgc accggcccga
     2041 actcgacgcc gacgccctgg ccgccgtggc gacaaacggc ttgctgctgg ccgaggctct
     2101 gggccggcaa atggagcgcg gcggcgcgct gcaaacgatc atcgagtacg aaggcggcgt
     2161 ggtcctcatc gagccgctac tgcgacgggt cctgccgcgt ggcgatcgcc gacgccgcgc
     2221 accggaccgt cgaggcctga gccgcgcgtc gtcgacctgc aagcg
//
LOCUS       DASUYC010000279         3200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1685606, whole genome shotgun sequence.
ACCESSION   DASUYC010000279 DASUYC010000000
VERSION     DASUYC010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1685606"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2837)
                     /locus_tag="VF276_04560"
     CDS             complement(<1..2837)
                     /locus_tag="VF276_04560"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEX6606168.1"
                     /translation="MFKSPVPERGGQWRALGIVGALLLLLAGALGALPGVLGARVAAM
                     QAASPAAQTGTPILQRTTGRSLKNDVSLPLRQIPPAKRIPVRSQENERIPFRNNRPLP
                     AVKDAVLQNWFRLGVAAMPTPSLSFEGNTNRGGFTPPDTNGDVGPNHYVQTVNVDLAV
                     WNKAGTLLYGPVPVNTLWYGFGGTCEFTNDGDPNTVYDPIADRWVISQFGVPGDVRGY
                     HECIAVSTSPDPAGSYNRYDFFLSPTLFHDYPQLGVWPDGYYASFNVFNPDNTFNSGA
                     WVTFDRDQMLAGQPAGYQEFNSPEDGGMMPSDLDGTDLPPPGAPNTFVEPFDPAGTLR
                     LWNFYVDWTNPANSHVAGPIAVPVAAWNPYCPGSDQCVPQPGTAQLLDPVSDGRIMRR
                     LAYRNFGGEYQSWVANFTVSGGPPAGHGISGVAAPRWFELRSAGAFTPTLSQEGTYAP
                     DFTNRWMASIAQDRDGNMAMGYSVSSSAVFPGIRYTGRLASDPPGTLPQGEVSLVDGG
                     GSQTGSNRWGDFTNIAVDPVDDCTFWYTNEYYPVTSPNLWQTRIGTFKFPTCGSGGTP
                     PPVTTTPAPSATPHNTPTSTPGPPATATPIPIVACAVNAFTGAIVQGDALQTGQIQPD
                     LYASTCDVPKGPRTLQDTQPRRYDAYTFYNATGSPQCVTIRLTTACAYDNNHALTSAA
                     YLDSFNPADITQNYLGDLGYTAPTLTEYQITVPANRAYVVVVSEYGAGTGCPSYSLQV
                     SCGVAGGTPTPTVTNTPTNTPTPTATDTPTVTPTPTATDTPTVTPTPTATDTPLPTPT
                     PFCDITFTDVPVEYWAYGYIKWAFCGGIVSGYADNTFRPEANASRGQVAKMVMLAGHY
                     DLTIPPGAPHFSDVPPENAFYPYIEAAYRLGVISGYDDGTFHPYGLISRAQLTKIVVA
                     ARGYPLENPVTPTFNDVPPTYWAYPYIETAFT"
BASE COUNT          493 a          922 c         1228 g          557 t
ORIGIN      
        1 gtgaaggccg tctcgatata ggggtaggcc cagtaggtcg gcggcacgtc gttgaaggtc
       61 ggcgtgaccg ggttctccag cgggtagccg cgcgccgcga cgacgatctt ggtcaactgg
      121 gcgcgcgaaa tcaggccgta gggatggaac gtgccgtcgt cgtagccgct gatgacgccg
      181 agccggtagg cggcttcgat gtaggggtag aaggcattct ccggcggcac gtcgctgaag
      241 tggggcgcgc ccggcgggat cgtcaggtcg tagtgtccgg cgagcatgac catcttggcg
      301 acctgcccgc gcgaggcgtt ggcctcgggc cggaacgtgt tgtcggcata gccgctgacg
      361 atgccgccgc agaaggccca cttgatatag ccgtaggccc agtactcaac cggcacatcg
      421 gtaaaggtga tgtcgcagaa gggcgtcggc gtcgggagcg gagtgtcggt cgccgtcggc
      481 gtcggggtca cggtaggcgt gtcggtggcc gtcggcgtcg gggtcacggt gggcgtgtcg
      541 gtggccgtcg gcgtcggggt gttagtgggt gtgttggtca cggtgggcgt gggcgtcccg
      601 ccggccaccc cgcaactgac ctggaggctg tagctagggc agccggtgcc cgcgccgtac
      661 tcggacacga cgaccacata ggcccggttg gccggcaccg tgatctggta ttcggtcagt
      721 gtgggcgcgg tgtagccgag gtcgcccagg taattctggg tgatgtcggc gggattgaag
      781 ctgtccagat aggccgcgct ggtcagcgca tggttgttgt cgtaggcgca ggccgtggtg
      841 agccggatgg tgacgcactg agggctgccg gtcgcattgt agaaggtgta ggcgtcgtaa
      901 cggcgcggct gggtatcttg tagggtccgc gggcccttgg gcacgtcgca ggtgctggcg
      961 tacaggtcgg gctggatctg cccggtttgc agcgcgtcgc cctggacgat ggccccggtg
     1021 aaggcgttca ccgcgcaggc cacgatgggg atgggcgtgg ccgtggccgg tggaccgggc
     1081 gtgctggtcg gcgtgttatg cggcgtcgcg ctgggcgcgg gcgtagtggt gacgggcggg
     1141 ggcgtgcccc cgctgccgca ggtggggaac ttgaacgtgc cgatgcgcgt ctgccagagg
     1201 ttgggcgacg tgaccgggta gtactcgttg gtgtaccaga aggtgcaatc gtccaccgga
     1261 tcgacggcga tattcgtaaa gtcgccccag cgattgctgc cggtttgcga gccgccgccg
     1321 tcgaccaggc tgacctcgcc ctgcggcagc gtgcccggcg gatcgctcgc caggcgaccg
     1381 gtgtagcgaa tgccggggaa cacggcgctg ctggacacac tgtagcccat cgccatgttg
     1441 ccgtcacggt cctgggcgat gctggccatc cagcgattgg tgaaatcggg cgcgtaggtg
     1501 ccttcctggg acagggtcgg cgtaaacgcg ccggcgctgc gcaactcgaa ccagcgcggg
     1561 gcggccaccc cgctaatacc gtggccggcg ggcgggccgc cgctcacggt gaaattggcg
     1621 acccaggact ggtactcgcc gccgaagttg cggtaggcca gccggcgcat gatgcgcccg
     1681 tcggacaccg ggtcgagcaa ctgggcggtg ccgggctggg gcacacattg atcgctgccg
     1741 gggcagtagg ggttccaggc tgccacgggc acggcgatgg ggccggcgac atgggagttg
     1801 gccgggttgg tccagtccac gtagaagttc cagagccgca gcgtccccgc cgggtcgaat
     1861 ggttcgacaa aggtgttggg cgcgccgggc ggcggcagat cggtgccgtc caggtcgctc
     1921 ggcatcatac cgccgtcctc ggggctattg aactcctggt acccggcggg ctgcccggcc
     1981 agcatctgat cgcggtcgaa ggtgacccag gcgccgctgt tgaacgtgtt gtcggggttg
     2041 aacacgttga acgaggcgta gtacccgtcc ggccagaccc ccagttgcgg atagtcgtgg
     2101 aagagggtcg ggctgaggaa gaaatcgtag cggttatagc tgccggccgg gtcgggcgat
     2161 gtggagacgg cgatacattc gtggtagccg cgcacgtcgc ccggcacgcc gaactggctg
     2221 atgacccagc ggtcggcaat cgggtcatat accgtattcg ggtcgccgtc gttggtgaat
     2281 tcgcaggtgc cgccgaagcc gtaccagagc gtattaaccg gcaccgggcc gtagagcagg
     2341 gtaccggcct tgttccagac ggccaggtcc acgttgaccg tctggacata gtgattgggg
     2401 cccacgtcgc cgttggtatc gggcggggtg aagccgccgc gattggtgtt gccctcgaag
     2461 ctcaagctgg gggtgggcat cgccgcgacc ccgagccgga accagttctg caacacggcg
     2521 tctttgacgg cgggcagggg ccggttgttg cggaagggga tccgctcgtt ctcctggctg
     2581 cggacaggaa tccgtttggc cggtgggatc tggcgcaggg ggagcgagac atcattttta
     2641 agggaccggc ccgtggtgcg ctggaggatc ggagtcccgg tttgcgccgc ggggctagcg
     2701 gcttgcatgg cggcgacccg cgcccccagg acaccgggga gcgcgcccaa ggcgccggcc
     2761 agcaggagca ggagcgcccc gacgatgccg agcgcgcgcc attgtccccc tcgttcaggc
     2821 acaggagact tgaacataga actttccttc ctgatgcttc ccgcgatacg aaagtgggtc
     2881 tggtgtgccg gctactaacg ctgccctcct accacgacgc gcaatcgggc cgtgcgccgt
     2941 ggaacgcacc gggtcgtcac aaaccgctac tatgggaccg gctgccggcc atcaggggga
     3001 gaaatctgca tcgcgcgccg ggccaaccgg acggcgcgag acttctatac aggcagtata
     3061 gcacaaggtg tgcgcgaggt gaagatgtga atcgaggaat tgtggcggag taggggacgc
     3121 ccggcccgtt caggggccgg gcgtcgctgg tgggcagggt aagcggctac agcagttctt
     3181 tgagtttctc ggcggcgcgg
//
LOCUS       DASUYC010000280         3209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_260349,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000280 DASUYC010000000
VERSION     DASUYC010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260349"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..1745
                     /locus_tag="VF276_04565"
     CDS             138..1745
                     /locus_tag="VF276_04565"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013919973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEX6606169.1"
                     /translation="MNMDQNGARRAIASGKTALGVEFGSTRIKMVLIGEDHAPIAAGG
                     YEWENRLENGIWTYHLDDVWAGLQESYRRLGAEVAVKYGVRLRTAGAIGVSAMMHGYL
                     AFDKEGNLLAPFRTWRNTMTGQAAEKLTELFQFNIPQRWSIAHLYQAILNHEPHVADI
                     SYLTTLAGYVHWQLTGRKVLGVGEASGMFPIDHATNNYDERLIDLFNERVEVQKLPWR
                     LEQILPRVLLAGDAAGRLSEEGARLIDPSGQLRAGIPLCPPEGDAGTGMVATNSVAAR
                     TGNVSAGTSVFAMIVLEQALSRLYPEIDMVTTPTGLPVAMVHSNNCTSDLNAWVELFA
                     EFSTALGVEIGNARVFEILYKQALAGDSDGGGMLAYNYLSGEHITHLPEGRPLFVRTP
                     TSRFTLPNFMRVHLFSAVGALKIGLDILFEREHVQLQRVLGHGGFFKTTEVGQKIMAA
                     AINVPVAVMETAGEGGAWGIALLAAYMLHRGTDEALETYLADNVFAGEQSTTVAPDLA
                     HVAGFAAFMERYKQGLVIEQVAVDALK"
     gene            1776..2420
                     /locus_tag="VF276_04570"
     CDS             1776..2420
                     /locus_tag="VF276_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013291260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEX6606170.1"
                     /translation="MLEALKEELLQLHLELARHGLVVWTGGNVSARDPATGLVAIKPS
                     GVRYESLRPEQMVVLDLDGNIIEGDLKPSSDTWSHLYIYRHRSDVNGVVHTHSRYATA
                     FAAVGKPIPVVLTAIADEFGGSIPCGGFSLIGDENIGKLVVESIGNSCAVLLKNHGVF
                     TVGKSAGAAVKAAVMTEDVAAAVWLALQIGTPDEIPADAVAKLHERYTNVYGQK"
     gene            2497..>3209
                     /locus_tag="VF276_04575"
     CDS             2497..>3209
                     /locus_tag="VF276_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015811865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HEX6606171.1"
                     /translation="MIDLKQFEVWFVTGSQHLYGPETLDQVAEHSTTLARALDEAASI
                     PVQVVFKPVMTGPDEIRDLCLQANTAPDCVGLVVWMHTFSPAKMWIAGLSALQKPFLH
                     LHTQYNRAIPWAAIDMDFMNLHQSAHGDREFGFIGSRLRLERKVVVGYWQDADVQQSL
                     GTWARAACAWADWQGARVVRCGDNMREVAVTDGDKIEAQRRFGYAVNGYGVSDVVQYV
                     NAVADAEVDGLIREYEDRY"
BASE COUNT          625 a          995 c         1032 g          557 t
ORIGIN      
        1 tggtcctcat cccgccacag ctcatcgtcc gtcaaagcac cagccctgat acgtctgggg
       61 ggaacgcaaa tcaatagcgc atcaagcggc gcgctgaaca cagcggtcgc ccagattatt
      121 ccctactctg ccgggagatg aacatggatc agaacggtgc ccgccgggca atcgcaagcg
      181 gaaagactgc gcttggcgtt gagttcggct caacgcgtat caagatggtg cttatcggtg
      241 aggaccatgc cccgatcgcg gcgggcggct acgagtggga gaaccgcctc gaaaacggca
      301 tctggaccta ccatctcgat gatgtctggg cggggctgca agagagctac cgccggctcg
      361 gcgccgaggt cgctgtaaag tacggggtgc ggctgcgaac cgccggtgcg atcggcgtga
      421 gcgcaatgat gcatggttac ctggctttcg acaaggaagg caacttgctg gccccgtttc
      481 gcacctggcg taatacgatg accgggcagg cggccgagaa gctgaccgag ctgttccagt
      541 tcaacattcc gcagcgctgg agcatcgccc acctctacca ggccatactc aaccacgaac
      601 cccacgtggc ggacatcagt tatctgacca cgctggccgg ttacgttcac tggcaactga
      661 cggggcgaaa ggtgcttggc gttggcgagg catcgggcat gttccctatc gatcacgcga
      721 ccaacaacta cgacgagcgc ctgatcgatc tgttcaatga acgcgtcgag gtgcaaaaac
      781 tgccctggcg gctggagcag atcctgccca gggtactgct tgccggggac gccgccggac
      841 ggctgagcga agaaggcgcc cggctgatcg atcccagcgg ccagttgcga gccggcatcc
      901 cgctttgtcc gcccgagggc gatgcaggca ccggcatggt cgccactaac agcgtggccg
      961 cgcgcacggg caacgtctcc gccggcactt cggtcttcgc catgatcgtg ctggagcagg
     1021 cgctgtcgcg actctacccc gagatcgaca tggtgaccac acccaccggc ttgccggtgg
     1081 cgatggtgca tagcaacaac tgcacgtctg acctcaacgc ctgggttgag ctgttcgcgg
     1141 aattcagcac cgccctgggc gtggagatcg gcaatgcccg ggtgtttgaa atcctgtaca
     1201 agcaggcgct cgccggcgac tcggatggcg gcggcatgct ggcctacaac tatctttcgg
     1261 gcgagcacat cacccacctg ccggaggggc gcccactgtt cgtgcgcacc cccacgagcc
     1321 gcttcacact ccccaatttt atgcgcgtgc atctcttctc cgcagttggc gccctcaaga
     1381 tcggcctcga catccttttt gagcgggagc acgtgcagct ccagcgcgtg ctcgggcacg
     1441 gcggcttctt caagacgacg gaagtgggtc aaaagatcat ggcggcggcg atcaatgtgc
     1501 ccgttgcggt gatggagacc gccggcgagg gcggcgcctg gggcatcgcg ctgctcgccg
     1561 cctatatgct gcacaggggt acggacgaag cactggaaac gtatctcgcc gacaacgtct
     1621 tcgccggaga acagagcacg acggtcgcgc ctgatctcgc gcatgtcgcg ggcttcgcgg
     1681 cattcatgga acggtacaag cagggccttg tcatcgaaca ggtcgccgtg gacgcgctta
     1741 agtagcggat tcagcacaaa acgaggttga taaacatgct ggaagcattg aaggaagaac
     1801 tgcttcaact gcacctggag ctggcgcggc atggactggt cgtatggacc ggcggcaacg
     1861 tcagcgcccg cgatcccgcg accgggctgg tagcaatcaa gccgtcgggc gtgcgctatg
     1921 agagcctgcg tccggagcag atggtggtgc tcgacctgga cggcaacatc atcgagggcg
     1981 atctgaagcc atcctccgac acgtggagcc acctctatat ctaccgtcac cgctccgacg
     2041 tgaacggcgt cgtccacacc cactcccgct atgccaccgc cttcgccgcg gtcggcaagc
     2101 cgatcccggt ggtactcacc gcgatcgccg acgagttcgg cggctccatc ccctgcggcg
     2161 gcttttcgct gatcggcgac gagaacatcg gcaaactggt ggtcgagagt atcggcaact
     2221 cgtgcgcggt cttactgaaa aatcatggcg tgttcaccgt cggcaagagc gccggcgccg
     2281 cggtcaaggc cgccgtgatg accgaggatg tggccgccgc ggtctggctg gcgctgcaaa
     2341 tcggcacgcc cgacgagatc cccgccgacg ctgtggctaa actccacgag cgctacacta
     2401 acgtctacgg ccagaagtag gcgcgataga ctggcacttt tcgcgctaac gccggttcgc
     2461 ctggtaaaac gaaacgatcc attgaaaggg atgaagatga tcgatctcaa gcaatttgaa
     2521 gtctggttcg tgaccggcag ccagcatcta tacggcccgg agacgctgga tcaggtggcc
     2581 gagcattcca caacgctggc gcgggcgctg gacgaggcgg cgagtatccc ggtgcaggtg
     2641 gtgttcaagc cggtgatgac cgggccggat gagatcagag atctatgctt acaggcgaac
     2701 acggcgcctg attgcgtcgg gctggtggtg tggatgcaca ccttctcgcc ggccaagatg
     2761 tggatcgccg gcctgtcggc gctacaaaag ccgttcttac acctgcacac gcagtacaac
     2821 cgggcgatcc cttgggccgc gatcgacatg gacttcatga acctccacca gtcggcgcac
     2881 ggcgaccgcg agttcgggtt catcggcagc cgcttgcgcc tggagcgcaa ggtcgtcgtc
     2941 ggctactggc aagatgccga tgtgcagcag agcctcggca cctgggcgcg agcggcctgc
     3001 gcctgggccg actggcaggg cgcccgggtg gtccgctgtg gcgacaatat gcgcgaagtg
     3061 gcggtcaccg acggcgacaa gatcgaggcg cagcgccgct tcggctacgc ggtcaacggc
     3121 tacggcgtca gcgacgtggt ccagtatgtg aacgccgtgg ccgacgccga ggtcgatgga
     3181 ctgatccgcg agtacgagga ccgctacca
//
LOCUS       DASUYC010000281         4400 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1065729, whole genome shotgun sequence.
ACCESSION   DASUYC010000281 DASUYC010000000
VERSION     DASUYC010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4400
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065729"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VF276_04580"
     CDS             <1..251
                     /locus_tag="VF276_04580"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HEX6606172.1"
                     /translation="TFWAPILIAGNIPIIGGILGFVGGLYSLYLTYLALQPTHRMDST
                     KAMTVVGILIAIAVVLGLCAFFTVGLAFLGLAAAGGGR"
     gene            complement(401..577)
                     /locus_tag="VF276_04585"
     CDS             complement(401..577)
                     /locus_tag="VF276_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606173.1"
                     /translation="MNQASANQVIWGLVGIFLALGLAAFVLDWGSAVVRILGVLVVLL
                     VLYQVVKRQGNAAR"
     gene            781..1269
                     /locus_tag="VF276_04590"
     CDS             781..1269
                     /locus_tag="VF276_04590"
                     /inference="COORDINATES: protein motif:HMM:NF026066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX6606174.1"
                     /translation="MAMTSQEAAEILFNCATLLEMAGANQYRVAAYRNAARVMLQLGD
                     LAPRVVANDEAMRGLHFGKRLTRKLRELFAENHMTFYDELLAELPLPVARLMTVPGVG
                     PKTALRLYNELGISSPRALAAAAGAGEIRKVHGFGPRREARWAAAGAVSELPAAELPK
                     AA"
     gene            1271..2731
                     /locus_tag="VF276_04595"
     CDS             1271..2731
                     /locus_tag="VF276_04595"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012187660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX6606175.1"
                     /translation="MARRARKPTSSAEAWLVPLPGFDLDLGDDDILAAFTDLDATDDA
                     GEGDVPEAAAPEPEPPPPDPFEVARERFALAATPALSLEPRAYQRAALQAWVGNGGRG
                     MVVLPTGAGKTVLALMAVAHAGLRTLVVVPTIELLQQWRDSLVHKLGLPEEMVGMIGG
                     GVRQSRPITVITYDSASMPRRKLDTWGLLIFDEAHHLPANAYRRIAEHSPAPLRLGLS
                     ATPERSDGLHEDLFTLIGQEVYRQGPKALSRDKHIAAYRERRIFVNLTGEEQARYDAL
                     TAEYRWYLATHRADLGAAYGAGMFEALIRRSGFDPAARQALAAQRKARMLALNAESKI
                     QAVEELLRKHAGDKVIIFSEFNVLVDSISKRLLLPSITYRTPAEERKATLAAFRTGAY
                     TKLVTGRVLNEGVDVPDANVAIVVSGSSTPREYIQRLGRVLRPKPGEAVLYEIITRGT
                     SEERSSRRRRPKEDLDPAPAAPAAPPETPAAVAPAA"
     gene            2962..>4400
                     /locus_tag="VF276_04600"
     CDS             2962..>4400
                     /locus_tag="VF276_04600"
                     /inference="COORDINATES: protein motif:HMM:NF017442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF790 family protein"
                     /protein_id="HEX6606176.1"
                     /translation="MTASFNRQNLKKTVRRSAGDGRTYIYPHRIVNGPAARGREVRAQ
                     LAIAIRYFETMVGQRRAALDPEALVALFGDHKLARGLVAAMARYYRYRPLQYSEVVPV
                     AVADVLFEKRLGTPAALRANLFRFLNTAPRAGFATEGDRADILSDFGGDLGLDPEQLA
                     ELLWLDSEENWVLTRLATPDPADLIALYDFLALETVLRYASKLELEFRTPVAAAVGRD
                     LRLLLGYYGLQCDLEEERAGRPWRVTLHGRADARGSWARHGKRLVRVLVRLLTAHPGC
                     LESGEAQIELGNASTVLRMDAPVLAQLGAAPDGVGADVPSVLTPAACADLRAAGLPSK
                     WALRLDPEPLVYAGGVLAPLALCMRQNRRVYLLPVESQATLDRVERALPHLRGRADLL
                     LLAAPGVAWPEGRAPAPLLARGPDDTLDLQTVIALLEQHWGQEAPVPAVTEDISPLTA
                     LLGRVRREGLVSAAEALAVLGEAPAAGPLP"
BASE COUNT          670 a         1561 c         1488 g          681 t
ORIGIN      
        1 cgaccttctg ggcgcccatc cttatcgccg gcaatatccc gatcatcggc ggcattctcg
       61 gcttcgtggg cgggctgtac tcactgtacc tgacctacct ggccttgcag ccgacccatc
      121 gcatggacag cacgaaggcg atgaccgtgg tgggcatcct gatcgctatt gccgtcgtgc
      181 tggggctgtg cgcgttcttc accgtgggcc tggctttcct gggcctggcg gcggcgggcg
      241 gcggccgcta atcttctcgc caaccgtaaa gacccggcct ccacagaggc cgggtctctt
      301 tttgtgccta aggccggcag gggcaccggg tatggctacc tacccgttgg accaggagac
      361 gccgtaggaa cgggccattt cccctacccg ccatcgcacc ttacctcgcc gcgttgccct
      421 gccgcttcac cacctggtac agcaccagca gcaccaccag cacgcccaga atccgcacca
      481 cggcgctgcc ccaatcgagc acgaaggcgg ccagccccag cgcgaggaag atgcccacga
      541 gaccccagat tacctggttg gcggacgcct gattcatccc ttttctacct ctttcaccgg
      601 tccagtttga gctacatatt tccgctttac tggacaaatc cccataggga tggtataatt
      661 atttagaacg gaagttctaa aaatggccga gttcgccgcc tgcgcggccc tgttggtagc
      721 acgtagcaaa gtccctgtag agatgtatat cagtctgtga tgagagaaag aggcacaccg
      781 atggcgatga cttcccagga agcggccgag atcctgttta attgtgccac gttgctggag
      841 atggccgggg ccaaccagta tcgggtggcg gcctaccgca acgccgcccg cgtcatgctc
      901 cagttgggcg atctggcccc gcgcgtggtc gccaacgacg aggcgatgcg cggcctgcac
      961 ttcggcaagc ggctgacgcg caagctgcgc gaactgttcg ccgagaacca catgaccttc
     1021 tacgacgagt tgctggccga gttgccgctg cccgttgccc ggctcatgac cgtgccgggg
     1081 gtcggcccca agaccgccct gcggctctat aacgagttgg gcatcagttc gcccagggcg
     1141 ctggccgccg cggccggggc gggggagatc cgcaaggtgc atggcttcgg gccgcggcgc
     1201 gaggcgcgct gggccgccgc cggcgcggtg agcgagctgc ccgccgccga actgccgaag
     1261 gcggcctgac atggcccgcc gcgcccgcaa acccacctcg tcggcggagg cctggctcgt
     1321 cccgctgccc ggcttcgacc tggacctggg cgacgacgac atcctggccg ccttcaccga
     1381 cctggatgcg accgacgacg ccggcgaggg ggatgtgcct gaagccgccg cgcccgagcc
     1441 ggaaccccca ccgcccgatc ccttcgaggt ggcgcgggag cgcttcgccc tggccgcgac
     1501 cccggcgctg agcctggagc cgcgcgccta ccagcgcgcc gccttgcagg cctgggtcgg
     1561 caacggcggc cggggcatgg tcgtgctgcc cacgggcgcc ggcaagacgg tgctcgcgct
     1621 gatggccgtg gcccacgcgg gcctgcgcac gctggtcgtg gtccccacca tcgaactgtt
     1681 gcagcagtgg cgcgactcgc tggtgcataa gctcggcctg ccggaggaga tggtcggcat
     1741 gatcggcggc ggcgtgcgcc agtcgcgccc gatcaccgtc attacctacg actcggcgtc
     1801 catgccccgc cgcaagctcg atacctgggg cctgctcatc ttcgacgagg cccaccacct
     1861 gccggccaac gcctaccgcc gcatcgccga gcactcgccc gcgccgctgc gcctgggcct
     1921 gagcgccacg ccggagcgct cggacggcct gcacgaagac ctgttcaccc tcatcgggca
     1981 ggaggtctat cgccagggac cgaaggcgct gtcgcgcgac aagcatatcg cggcctaccg
     2041 cgagcggcgt atcttcgtca acctgaccgg cgaggagcag gcgcgctacg acgccctgac
     2101 cgccgagtac cgctggtacc tggcgacgca ccgcgccgac ctgggcgctg cctacggcgc
     2161 gggcatgttc gaggcgctga tccggcgcag cgggttcgac cccgccgccc ggcaggcact
     2221 cgccgcccag cgcaaggcgc gcatgctggc cctcaacgcc gagtccaaga tccaggcggt
     2281 ggaggagctg ctgcgcaagc acgcgggcga caaggtgatt atcttctccg agttcaacgt
     2341 gctggtcgac tcgatcagca agcgcctgtt gctgccgtcg atcacctacc gcacgcccgc
     2401 cgaggagcgc aaggccaccc tggccgcttt ccgcaccggc gcctacacca agctggtgac
     2461 ggggcgggtg ctcaacgagg gcgtagacgt gcccgatgcc aacgtcgcca ttgtcgtcag
     2521 cggcagcagc acgccgcgcg aatatatcca gcgcctgggc cgcgtgttgc ggcccaagcc
     2581 gggcgaggcc gtgctctacg agatcatcac gcgcggcacc tcggaagaac ggtcgtcgcg
     2641 gcgccgccgg cccaaagaag acctggaccc ggcgcccgcc gcgcccgcgg ccccgcccga
     2701 aaccccggca gccgtggccc ccgccgcctg atcgtccggc gcgaggcaaa gtagtccgtc
     2761 acaccgaaat tgtcattctg agcggagcga agaatctccg tcttgcccag ccaacgacga
     2821 gactcttcgc tgggctcaga gtgacacagg agcagcattg tcattctgag cggagcgaag
     2881 aatctccgtc tcgctagacg agactcttcg ctgggctcag agtgacagat ctaccgggaa
     2941 gcggcataga ggaggagctt gatgaccgcg agctttaacc gccagaacct caagaagacg
     3001 gtccgccgct cggcgggcga cgggcgtacc tatatctacc cgcaccgcat cgtcaacggc
     3061 ccggcggcgc gcgggcgcga ggtgcgcgcc caactggcga tcgccatccg ctacttcgag
     3121 acaatggtgg gccagcggcg cgcggcgctt gacccggagg cgctggtcgc gctgttcggc
     3181 gatcacaaac tggcgcgcgg gctggtagcg gccatggccc gctactatcg ctaccgcccg
     3241 ctgcaataca gcgaggtggt gccggtggcc gtggccgatg tgctgtttga gaaacggctc
     3301 ggcaccccgg cggcgttgcg cgccaacctc ttccgcttcc tcaacaccgc gccccgcgcc
     3361 ggcttcgcca ccgagggcga ccgggccgat atcctgtccg atttcggcgg cgacctgggc
     3421 ctggacccag agcaactggc cgagctgctc tggttggaca gcgaggagaa ctgggtgctg
     3481 acccggctcg ccacacctga ccctgccgac ctgatcgcgc tctacgactt cctcgcactg
     3541 gagacggtgc tgcgctacgc cagcaagctc gaattggagt tccgcacgcc ggtcgccgcg
     3601 gcggtgggcc gcgatctgcg cctgctgctg ggctactacg gcttgcagtg cgacctggag
     3661 gaagagcgcg ccgggcggcc ctggcgggtc acgctgcacg ggcgggccga tgcgcgtggc
     3721 tcctgggccc gccacggcaa gcgcctggtg cgggtcctcg tgcgcctgct gacggcgcac
     3781 cccggctgcc tggagagcgg cgaagcgcag atcgagttgg gcaacgcctc caccgtgctg
     3841 cggatggacg ccccggtgct ggcccagttg ggtgccgcgc ccgacggggt cggggcggat
     3901 gtgccgtccg tgctgacgcc cgcggcctgc gccgatctgc gggcggcggg tttgccaagc
     3961 aagtgggcgc tgcggctcga tcccgaaccg ctggtctacg cgggcggcgt gctggcgccg
     4021 ctggcgctct gcatgcgcca gaaccggcgc gtgtacctgc tgccggtcga gagccaggcc
     4081 acgctggatc gcgtcgagcg cgcgctgccc catctgcgcg ggcgggccga tctgctgctg
     4141 ctggccgcgc ccggcgtggc ctggccggaa ggccgggcgc ctgcgccgct gctcgcccgc
     4201 ggaccggacg ataccctcga tctccagacc gtgatcgccc tgctggagca gcactggggc
     4261 caggaggcgc ccgtgcccgc cgtcacggaa gacatctcgc cgttaaccgc gctgctgggg
     4321 cgggtgcgtc gcgagggctt ggtgtccgcc gccgaggcgc tggcggtgct gggcgaggcg
     4381 cccgccgccg ggccgctgcc
//
LOCUS       DASUYC010000282         3862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_656731,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000282 DASUYC010000000
VERSION     DASUYC010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656731"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..1023
                     /gene="purM"
                     /locus_tag="VF276_04605"
     CDS             13..1023
                     /gene="purM"
                     /locus_tag="VF276_04605"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX6606177.1"
                     /translation="MMRARVRATFGPEVLADLGHFSGLYNLGPRLGLPPGDRDPVLVA
                     SADGVGTKLRLAILAGRHATIGADLVNHCVNDILTCGARPLFFLDYFATGTLDPAQAA
                     AVVGGLAAACRAVGCALLGGETAEMPGFYAPGDYDLAGFIVGLVERGRMVLGTAIAPG
                     DVVLGLPAAGLHTNGYSLARRALGLDDPDAAVALARMAEQPPALAAPLGDVLLAPHRC
                     YLPVVAPLLDAPGGPLVRGMAHITGGGLPGNIARVLPPGTGVVLDQGTWPVPPIFGLI
                     AERGAVPVAEMPQVFNMGLGFILIAAPEQVGAIRDLAPEALVVGQVVARPDPAAPAVV
                     LR"
     gene            1031..1735
                     /gene="purN"
                     /locus_tag="VF276_04610"
     CDS             1031..1735
                     /gene="purN"
                     /locus_tag="VF276_04610"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX6606178.1"
                     /translation="MDDDARDPAAQLPLFSAPSTPPPPVVVPAPALPLRLGVLVSGRG
                     SNLRVLLEACTAGTLPATVVAVVSNHSDVPALDIAAEYGVPSTVIPRAGSPSRLAQQQ
                     QMADFLDAAGAQLIVCAGFDRVLEAAICEHYLGRMINLHPSLLPAFGGGLHAIADALA
                     YGVKVTGVTVHFVTTDIDAGPIILQEAVPVLPDDTLASLTARVQAVEHRLLPAAVELY
                     ALGRLAVEGRRVLIRP"
     gene            1996..2490
                     /locus_tag="VF276_04615"
     CDS             1996..2490
                     /locus_tag="VF276_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEX6606179.1"
                     /translation="MDPQLTSLVVSEKDTDAIEECLERLVGDTNANYSMLLDKSGQVI
                     AARGDTQRQDITALGALLAGTFASSREVARLLREKDFRVLFQQGVKENIFTELIEEQW
                     MLVVMFDKRTHIGLVKVLAKRATDELGSILQRVRQQSRQRDAVISTSFRTSVEDTIDL
                     LFRD"
     gene            2503..3078
                     /locus_tag="VF276_04620"
     CDS             2503..3078
                     /locus_tag="VF276_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding-motility protein MglA"
                     /protein_id="HEX6606180.1"
                     /translation="MALINVAAREIHCKIVYYGPGLCGKTTNLKYIHSMVPQQAKGDL
                     ISIATETERTLFFDFLPLDLGQVHGFQTRIHLFTVPGQVLYERTRVAVLNGADGVVFV
                     ADSQRHKLQEDVQSLQELARNITLMGKKFLEFPLVMQYNKRDLENALPVEVMDRYLNS
                     IRVPRYEAAAAKGIGVFDTLKAVSKLVISKL"
     gene            3097..>3862
                     /locus_tag="VF276_04625"
     CDS             3097..>3862
                     /locus_tag="VF276_04625"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX6606181.1"
                     /translation="MALEGTLKDFPLPDIIQLLSLSRKTGAVEIMGSDGFGSGRLYFH
                     QGKVVSAALEDMAPLDAAYSFFTFPGGNFRFYENELPKADAQPITISNEFLIMEGIRR
                     ADEWQKLRDRVPSLDLVLGLVADPVAGNRDINLKPDEWRVLTMINGRDSVRQIARRTS
                     FGDFKTARIIYHLLSAGLIEPLGVPGVPAAPVAAPAPAPAPVTAPVAPPASFEAPVRV
                     PPAPPVEPARPAPVADQVIRRIAPAPAPAPPAPPPAR"
BASE COUNT          597 a         1340 c         1276 g          649 t
ORIGIN      
        1 gaggccgtcg agttgatgcg cgcccgcgtg cgggccacct ttggccccga agtgctggct
       61 gacctgggcc atttcagcgg cctgtacaac ctgggccccc gcctgggcct cccgccgggc
      121 gaccgcgacc cggtgctggt cgccagcgcc gacggcgtcg gcaccaaatt gcgcctggcg
      181 atcctggccg ggcgccacgc aaccatcggc gccgacctcg tcaaccactg cgtgaacgac
      241 atcctcacct gcggggcgcg cccgctgttc ttcctcgact atttcgccac cggcacgctc
      301 gacccggccc aggccgccgc cgtggtcggg gggctggccg cggcctgccg ggcggtgggc
      361 tgtgccctgc tcggcgggga gacggcggag atgcccggtt tctacgcgcc gggggactat
      421 gacctggccg ggttcatcgt gggcctggtg gagcgcgggc gcatggtgct gggaacggcc
      481 atcgcgccgg gcgacgtggt gctgggcctg ccggcggcgg ggctgcacac caacggttac
      541 tcgctggccc gccgcgccct gggcctggac gatcccgatg ccgccgtcgc cctcgcgcgg
      601 atggcagagc aaccacccgc gctggcggcc ccgctgggcg atgtgctgct ggcgccccat
      661 cgctgctacc tgccggtggt ggcgccgctg ctggacgcgc ccggcgggcc gctggtgcgc
      721 ggcatggccc atatcaccgg gggcgggctg ccggggaata ttgcgcgggt gctgccgccg
      781 ggcaccggcg tcgtcctgga tcagggcacc tggcccgtcc cgccgatctt cggcctcatc
      841 gccgagcggg gcgcggtgcc cgtcgccgag atgccccagg tgttcaatat gggcctgggc
      901 ttcatcctca tcgccgcgcc cgaacaggtc ggggcgattc gcgacctggc gccggaggcg
      961 ctggtcgtcg ggcaggtggt ggcgcggccc gacccggcgg cgcccgccgt ggtgctgcgg
     1021 tagcccgccc atggacgacg acgcccgcga ccccgccgcg cagttgccgt tgttctcggc
     1081 gcccagcacg ccgccgccgc ccgtggtggt gcccgcgccg gcgctgccct tgcggctggg
     1141 ggtactcgtg tcgggccggg gcagcaatct gcgcgtgctg ctcgaagcgt gtaccgcggg
     1201 tacgctcccg gcgacggtgg tggccgtcgt ctccaaccac agcgacgtgc cggcgctcga
     1261 catcgccgcc gagtacgggg tgccgagcac ggtgatcccc cgcgcgggca gccccagccg
     1321 cttggcccag cagcagcaga tggccgattt tctcgacgcc gccggggccc aactgatcgt
     1381 ctgcgcgggc ttcgaccggg tgctcgaagc cgcgatctgc gagcattacc tggggcgcat
     1441 gatcaacctg cacccctcgc tgctgccggc cttcggcggc ggcctgcacg ccattgccga
     1501 cgccctggcc tacggcgtca aagtcaccgg tgtcacggtc catttcgtca ccaccgacat
     1561 cgacgccggg ccgatcatct tgcaggaggc cgtgccggtg ctgcccgacg ataccctggc
     1621 gagcctcacc gcccgtgtgc aggcggtgga acatcgcctg ctgcccgcgg cggtcgaact
     1681 ctacgcgctg gggcggctgg cggtggaagg ccggcgcgtg ctgattcgcc cctgagcggg
     1741 cgccattcgg ggccaatcgg ctaccggcaa ccgggaccaa tggctacccc gcccgcccta
     1801 ccgctcatgg tatgcttata tatcgagaga cccggccggc gtgcctgcgc acgcgtgttt
     1861 gttggcgccg tggcgctccg ccgttattat tgacatatca taaagcctag tgtatagtaa
     1921 ggcagatggc acattggacc gcctgaaccg tctcaacacg tcgccgcacc gccgcaacag
     1981 agggaggctc cccgtatgga cccgcagtta acgagcctgg tcgtgtcgga gaaagacacg
     2041 gacgcgatcg aggagtgcct cgaacgtctc gtgggggaca cgaacgccaa ctactccatg
     2101 ttgctcgata agagcggcca ggtcattgcg gcgcgcggcg acacccagcg acaagatatc
     2161 accgccctgg gcgccctgct ggccggcact ttcgcgtcct cccgcgaagt agcccggctc
     2221 ttgcgcgaaa aggatttccg tgttctcttc caacaaggtg tcaaagaaaa tatcttcacc
     2281 gagctgatcg aagagcagtg gatgctggtt gtgatgtttg acaagcgaac ccacatcggc
     2341 ctggtcaagg ttcttgccaa gcgcgcgacc gacgaactcg gttccattct gcaacgcgtg
     2401 cgccagcaga gccgccagcg tgatgccgtc atcagcacca gtttccgcac ctcggttgag
     2461 gataccatcg atttgctctt ccgcgactaa tggtgaggta tcatggcact gattaatgtg
     2521 gccgcccgcg aaatacactg caaaatcgtg tactatgggc cgggactctg tggcaagacg
     2581 acgaacctca aatacatcca ctccatggtg ccccagcagg ccaagggcga cttgatttcc
     2641 atcgcgaccg aaaccgagcg cacgctcttt ttcgacttct taccgctgga tctcgggcag
     2701 gtgcatggct ttcagacgcg catccacctg ttcaccgtgc cgggccaggt gctctacgaa
     2761 cgcacccgcg tggccgtgct gaacggcgcc gacggcgtcg tcttcgtggc cgactcgcag
     2821 cgccacaagt tgcaggagga tgtgcaaagc ctgcaggagt tggcccgcaa tatcacgttg
     2881 atgggtaaga aattcctcga atttccgctg gtgatgcagt acaacaagcg ggacctggaa
     2941 aacgccttgc cggtggaagt gatggaccgg tatctgaatt cgatccgggt tccgcgctat
     3001 gaagccgcgg ctgcgaaagg aattggcgtc ttcgatacgc tcaaagccgt cagcaaactg
     3061 gtcatcagca agctgtaaac agcgaggctc cgcaccatgg cgctggaggg tacgctcaaa
     3121 gacttccctc tgcccgacat catccagttg ctcagcctga gccgcaaaac gggcgcagtg
     3181 gagatcatgg gcagcgacgg cttcggcagc gggaggttat acttccacca ggggaaggtg
     3241 gtgtccgccg cgctcgaaga catggcgccg ctcgacgctg cctattcctt cttcaccttt
     3301 cccggcggca acttccgttt ctacgaaaac gaactgccca aggcggacgc ccagcccatc
     3361 accatctcca acgagtttct gatcatggag ggcatccgcc gggcggatga gtggcagaag
     3421 ctgcgcgacc gggtgccctc gctcgacctg gtgctcggtc tggtggccga cccggtggcc
     3481 ggcaaccgcg atatcaatct caagcccgac gagtggcggg tgctgaccat gatcaacggg
     3541 cgcgacagcg tccggcagat cgcccgccgc accagctttg gcgacttcaa gaccgcgcgc
     3601 attatttacc atctgctgag cgcggggctg atcgaaccgc tgggtgtacc aggggtgccc
     3661 gcggcgcccg tggccgcccc cgcgcccgcc cccgcgcccg tcacggcgcc cgtggcgccg
     3721 ccggcctctt tcgaggctcc ggtgcgcgtg ccgccggccc cgccggtgga gccggcccgg
     3781 cccgcgcccg tggccgacca ggtgatccgg cgcatcgcac ccgcgccggc accggcgccc
     3841 ccggctccgc cgcccgcgcg cc
//
LOCUS       DASUYC010000283         2107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_331433,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000283 DASUYC010000000
VERSION     DASUYC010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331433"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VF276_04630"
     CDS             complement(<1..373)
                     /locus_tag="VF276_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606182.1"
                     /translation="MSTRAPVGRERAALGPGRRLLWSIPLGWQLSALYIVLLAVTLSL
                     VGALVYTRQERFLVDDIANRLADEARRVMALPIGRQAQPGGGGGFPGDDNRQDPNRGY
                     PRGNASGPDGSFGPGEQTRLVT"
     gene            complement(370..1089)
                     /locus_tag="VF276_04635"
     CDS             complement(370..1089)
                     /locus_tag="VF276_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015209517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6606183.1"
                     /translation="MTTGESTATGINDGERLVRVLVVDDEPLIVQMLSIALSYEKFEV
                     SVARTGIEAIQQAARTKPDLIILDIMLPHMDGIEVCRRLRATGDVGILMLTARGEDQD
                     QVAGLDSGADDYLVKPFTLPVLLARMRAILRRKGINLQSTLRVGDLTLDRLTRKVARG
                     ERQVELTPREFDLLELLLAHPRQVFSREAIVNRVWGYDFVGDSNVVDVYVRYLREKLG
                     DEDRNLIRSVRGVGYSLEPSA"
     gene            1406..>2107
                     /locus_tag="VF276_04640"
     CDS             1406..>2107
                     /locus_tag="VF276_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009510835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX6606184.1"
                     /translation="MVVAADTAPYLDSTRPTAERVHDLIAHMTVEEKIGQLQNEAAAI
                     PRLDIPAYNYWNEALHGVARNGRATVFPQIIGMAATWDPALIRRAASAIADEARAKYH
                     EALRRHGYTQLYQGLTFWSPNVNIYRDPRWGRGQETWGEDPYLTGELGAAFVRGMQGD
                     DPRYLKTAACAKHYAVHSGPEKLRHGFDAIVSRHDLHDTYLPAFHAQVEAGVEAVMGA
                     YNRVNGEPCCAHPYLL"
BASE COUNT          359 a          739 c          690 g          319 t
ORIGIN      
        1 tggtcaccag ccgggtttgc tcgcccggcc cgaacgaccc gtcggggccc gacgcattgc
       61 cgcggggata gccgcgattc ggatcctgcc ggttatcgtc gccggggaac ccgccgccgc
      121 cgcccggctg ggcctggcgc ccgatcggca gcgccatgac ccgccgcgcc tcgtccgcca
      181 gccggttggc gatatcgtcc acgaggaagc gttcttgccg cgtgtagacc agcgcgccga
      241 cgagggacag ggtaacggcg agcagcacga tgtagagcgc gctcaactgc cagcccaggg
      301 ggatgctcca gagcagccgg cgccccggcc ccagggccgc ccgctcccgc ccgaccggcg
      361 cgcgtgtgct catgcgctcg gctccaggct gtagccgacg ccgcgcacgc tgcggatcag
      421 gttgcggtcc tcgtcgccca gcttctcgcg caggtagcgc acatacacgt ccaccacgtt
      481 actgtcgccc acaaagtcat agccccacac gcggttgacg atggcctcgc gcgaaaagac
      541 ctgccggggg tgcgccagca atagctccag caggtcgaac tcgcgcgggg tcaactccac
      601 ctgccgctcg ccgcgcgcca ccttgcgcgt cagccggtcc agcgtcaggt cgcccacgcg
      661 cagcgtgctt tgcagattga tgcccttgcg ccgcaagatg gctcgcatcc gcgccagcaa
      721 caccggcagc gtgaacggct tgaccaggta atcgtcggcg ccgctgtcga ggccggccac
      781 ctggtcctgg tcctcgccgc gcgccgtgag catgaggatg cccacatcgc cggtggcccg
      841 caggcggcgg cagacctcga tgccgtccat atgcggcagc atgatgtcga ggatgatcag
      901 gtcgggcttg gtacgcgccg cctgctggat ggcctcgatg ccggtgcggg ccacgctgac
      961 ctcaaatttt tcgtagctga gggcgatact gagcatctgc acgatcagcg gctcgtcgtc
     1021 caccaccagc acgcgcacca gccgttcgcc gtcattgatt cccgtcgccg tcgactcgcc
     1081 ggtagtcaca tttcgtctcc ttctttagca catgctggcc caataggcgc tgagcagcta
     1141 tactatgcaa cgttacacga tcattgtagt gccgccgggt tagagaaagg ccggagaaaa
     1201 attagaaatc gctgagaatg ttagcggccc ggcgccggta acgcgccctg acagctttcg
     1261 gcggtgtagg ggccgggatg caaccggccc gcgcctgtcc gccctgtagg ggccggttgc
     1321 atcccggccc gcggggtaca gcgcggcata gagtgtgcag attgccgctt ggttcgtgcg
     1381 gacaatggag acacaaggga ggggaatggt tgtggcagca gacacggcac catatctgga
     1441 cagcacgcgc ccgacagcgg agcgagtgca cgacttgatc gcgcacatga ccgtggagga
     1501 gaagatcggc cagttgcaga acgaggccgc ggcgatcccg cggctggaca tcccggccta
     1561 caactactgg aacgaggcgc tgcacggcgt ggcgcgcaac gggcgcgcga ccgtcttccc
     1621 gcagatcatc ggcatggccg ccacctggga cccggcgctg atccggcgcg ccgcatcggc
     1681 cattgccgac gaggcccgcg ccaaatacca cgaggcgctg cggcggcacg gctacacgca
     1741 actctaccag gggctgacgt tctggtcgcc caacgtcaac atctaccgcg acccgcgctg
     1801 ggggcgcggc caggagacat ggggcgaaga cccctacctg accggcgagt tgggcgcggc
     1861 cttcgtgcgc ggcatgcagg gcgacgaccc gcgctacctc aagaccgccg cctgcgccaa
     1921 gcactacgcc gtccacagcg ggccggagaa gctgcgccac ggcttcgacg ccatcgtgag
     1981 ccggcacgat ctccatgata cctacctgcc cgccttccac gcccaggtcg aggcgggggt
     2041 ggaggcggtc atgggcgcct acaaccgcgt caacggcgaa ccctgctgcg cgcatcccta
     2101 cctgctc
//
LOCUS       DASUYC010000284         3202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_708188,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000284 DASUYC010000000
VERSION     DASUYC010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708188"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1226
                     /locus_tag="VF276_04645"
     CDS             <1..1226
                     /locus_tag="VF276_04645"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6606185.1"
                     /translation="ARQAAAEQFGYIEERLSGVEDIQGSGASPYTLGRLFGFTRGVLR
                     TRRAAEMVEFLSAGMTNLLVGIGWALGLGIGAYLYTQQQITIGTVFLILAYIGLLTAP
                     LDSLRKQASNLQRATAGIGRIAALFRTQPTVHATGEAALPPGALAVEFDGVSFAYADE
                     APGDEPAADPEAGLVLRDVSFHLPAGRVLGVLGRTGSGKTTLTRLLFRLYDPTGGAVR
                     LGGVDIRTVAPTDLRARVGMVTQDVQLFSASLRDNIAFFDPAIDDARIIAALDALGLR
                     DWLAAMPQGLDTRLAPGGAGMSAGEAQLLAFTRLLLRDPGLVILDEAASRLDLITEGR
                     LDRAVTRLLDGEGGRRSAIIIAHRLHTVQRADDILILDGGRVVEYGPRARLAADPGSR
                     FYRLLQTGGVEEVLA"
     gene            1223..3073
                     /locus_tag="VF276_04650"
     CDS             1223..3073
                     /locus_tag="VF276_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6606186.1"
                     /translation="MSKHPADRSRKIGTAYYNWRLIRYAPWPLVVLIAGDLIFFGARV
                     APGLIEKAAFDRLTNAAPVQFDIPALIALYISVELARAVAYLGDAWGGWTFRGLAGAL
                     LQRNLFAAALRRPGALAPPVSSGEAVNRYRDDVGETADFPTWSGTNLGYLASFGVAVA
                     IMASINLTITLVIFLPLVVTILVSYLAWARWRVAWHTVGQTGDAVTGFLGELFGAVQA
                     VKVADAEHKVIRHLHTLNDNRRRAKIRAGGLQAVIQAFEGGSIDFGVGVILLLAGQAM
                     AAGTFTVGDFALFTYYLVFATDLPALVGNFIGDYQAQEVSIDRLVALVPDEAPEVLLE
                     HHPVYAHGGAPPAPTASAGPAAPLALLEVRGLTYHHPGSSHGIRAADLSLPGGSFTVI
                     TGRVGAGKTTLLRALLGLLPRESGAISWNGTPVADPAAFFVPPRAAYTPQVPRLFSAT
                     LRENILLGAPASAAGLEDAIHRAVLEPDVAGLERGLDTVVGPRGVRLSGGQVQRAAAA
                     RMLVRRPALLVCDDLSSALDVETEQALWDRVLGPPGAPPGARPTVLVVSHRRAALRRA
                     DQIIVLQDGAVTATGTLDTLLATSAEMRRLWAGDLGAPAPPAAPVQLAAP"
BASE COUNT          402 a         1226 c         1108 g          466 t
ORIGIN      
        1 ccgcgcggca ggctgccgcc gaacagttcg gctacatcga ggagcgcctg tccggggtcg
       61 aagacatcca gggcagcggc gcctcgccgt acacgctggg ccggctgttc ggcttcacgc
      121 gcggggtgct gcgcacgcgc cgcgcggcgg agatggtcga attcctcagc gccggcatga
      181 ccaatctgct ggtcggcatc ggctgggcgc tggggctggg tatcggggcc tacctctaca
      241 cgcaacagca gatcaccatc ggcaccgtct tcctcatcct ggcctacatc ggcctgctga
      301 ccgcgccgct cgacagtttg cgcaagcaag cgagcaacct ccagcgggct acggcgggca
      361 tcgggcggat cgcggcgctg ttccgcaccc agcccaccgt gcacgccacc ggcgaggccg
      421 cgctgccgcc cggcgctctc gcggtcgagt tcgacggcgt ctcgttcgcc tatgccgacg
      481 aggcaccggg cgacgagccg gcggcggacc cggaggccgg gttggtcctg cgcgacgtgt
      541 cgttccacct gcccgcgggg cgggtgctcg gcgtgctggg gcgcaccggc agcggcaaga
      601 ccaccctcac gcgcctgctg ttccgcctct acgaccccac cggcggcgcc gtccgcctgg
      661 gcggcgtgga catccgcacc gtcgctccga cagacctgcg cgcccgcgtc ggcatggtca
      721 cgcaagatgt gcagcttttc agcgccagcc tgcgcgacaa catcgccttc ttcgacccgg
      781 ccatcgacga cgcgcggatc atcgccgcgc tcgacgcgtt gggcctgcgc gactggctgg
      841 ccgccatgcc gcagggcctc gatacgcggc tcgcgccggg cggggcaggg atgtcggcgg
      901 gcgaggccca gttgctggcc ttcacgcgcc tgttgctgcg cgatcccggc ctggtcatcc
      961 tggacgaagc ggcctcgcgg ctcgacctga tcaccgaggg gcgcctggac cgcgccgtaa
     1021 cccgcctgct ggacggcgag gggggccgcc gcagcgccat catcatcgcc caccgcctgc
     1081 ataccgtgca gcgcgccgac gacatcctga tcctcgacgg gggccgcgtg gtcgaatacg
     1141 gcccgcgcgc ccggctggcg gcggaccccg gttcccgatt ctaccgcctg ctgcaaaccg
     1201 gcggcgtgga ggaggtcctc gcatgagcaa gcaccccgcg gatcggagcc gcaaaatcgg
     1261 caccgcgtac tataactggc gcctcatccg ctatgcgccc tggccgctcg tcgtgctgat
     1321 cgccggagac ctgatctttt tcggcgcgcg cgtggcgccc ggcctgatcg agaaagccgc
     1381 cttcgaccgg ctcaccaacg ccgcgccggt gcaattcgac atcccggcgc tgatcgcgct
     1441 ctacatctcg gttgaactgg cgcgcgccgt ggcctacctg ggcgacgcct ggggcggctg
     1501 gacgttccgc gggctggccg gggcgctgct acagcgcaac ctgttcgccg ccgcgctgcg
     1561 ccggcccggc gcgctggccc cgcccgtctc gtcgggcgag gccgtcaacc gctaccgcga
     1621 cgacgtgggc gagaccgccg acttccccac ctggagcggc accaatctcg gctacctggc
     1681 ctcgttcggc gtcgccgtcg ccatcatggc gagcatcaac ctgaccatca cgctggtcat
     1741 cttcctgccg ctggtggtca ccatcctggt aagctacctg gcctgggcgc gctggcgggt
     1801 cgcctggcac accgtgggcc agaccggcga cgccgtcacc ggcttcctgg gcgaactgtt
     1861 cggcgccgtc caggcggtga aggtggccga tgccgagcat aaggtgatcc ggcacctgca
     1921 cacgctgaac gacaaccggc gccgggccaa aatccgcgcc ggcggcctcc aggccgtcat
     1981 ccaggcgttc gagggcgggt caatcgactt cggcgtcggc gtcatcttgc tgctggccgg
     2041 gcaggccatg gccgcgggca ccttcaccgt gggcgatttc gccctgttca cctactatct
     2101 cgtcttcgcc accgacctgc ccgccctggt cggcaacttc atcggcgatt accaggcgca
     2161 agaggtgtcc atcgaccgcc tggtggcgct ggtgcccgac gaggcgccgg aggtcttgct
     2221 cgaacaccac ccagtttacg cgcacggcgg cgccccgccc gcccctaccg cctccgccgg
     2281 ccccgccgcg ccgctggcgc tgctggaggt gcgcgggctg acctaccacc atcccggctc
     2341 gtcacacggc atccgcgccg ccgacctgag cctgccgggc gggtcgttca ccgtgatcac
     2401 cgggcgcgtc ggcgcgggca agacgacgct gctgcgcgcc ctgctcggcc tgctgccgcg
     2461 cgagagtgga gccataagct ggaacggtac gccggtggcc gaccccgccg cgttcttcgt
     2521 gccgccgcgc gccgcctata cgccgcaagt gccgcgcctg ttcagcgcga ccctgcgcga
     2581 gaatatcctg ctcggcgcgc cggcctccgc cgccggcctg gaggacgcca tccaccgcgc
     2641 cgtgctcgaa cccgacgtgg ccgggctgga gcgggggctg gacacggtgg tcggcccgcg
     2701 gggcgtgcgg ctgtcgggcg gccaggtgca gcgtgccgcc gccgcgcgta tgctggtgcg
     2761 ccggcccgcg ctgctggtct gcgacgacct gtcgagcgcg ctcgatgtcg aaaccgagca
     2821 ggcgctgtgg gaccgcgtgc tggggccgcc cggcgccccg cccggcgccc gccccacggt
     2881 gctggtggtg tcgcaccgcc gcgccgccct gcgccgcgcc gaccagatca tcgtgctcca
     2941 ggacggcgcc gtgaccgcca ccggcacgct cgacaccctg ctggcgacca gcgccgagat
     3001 gcgccgcctc tgggccggcg acctcggcgc gcccgcgccc cccgccgcac ccgtgcagtt
     3061 ggccgcgcct tagccgggcg cggctcgccg ccgagcgccc tgtaggggcc ggtttgaaac
     3121 cggcccgcgg cggcaggcgg cgccgatttc gttgacccaa ggccggggag gtagagcggc
     3181 aggcgggttt gaaacccgcc cc
//
LOCUS       DASUYC010000285         2771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_648376,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000285 DASUYC010000000
VERSION     DASUYC010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648376"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2545)
                     /locus_tag="VF276_04655"
     CDS             complement(<1..2545)
                     /locus_tag="VF276_04655"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX6606187.1"
                     /translation="MLTTYRTSPLARHPAVRVPAVVLLLLACVLSLAPAPGVAGPATP
                     PHRRAVAKPRPVPPPAQPADWQAGQLLVQLAPAQRLRLDAAGTIQAGGAELRPALAAW
                     GLNRVEVLDAGSGLYRLHADQPGLDVAGAARALAALPGVVYAEPDYRLHAAFVPSDPL
                     FSRQWALNQVGAPAAWDITTGSPDVIVAMIDTGIAPNHPDLVGKLVPGYNFVADTTTA
                     ADDNGHGTYTAGLVGALSNNNIGVAGVAWATRIMPVKILDKDGAGNVGDFSRGIHFAV
                     DHGAKVINISAGIEYPASSMQEAVRYARDHGVLVVASAGNTPDGAARYPAGFDEVVAV
                     SATDEQDKTAAFSSYGAFVDLAAPGVNIVSTGWGQGRAGYTWASGTSSAAPLVTGAAA
                     LLLAARPDLTPDDLQRLLEDGADDLGPAGWDPHYGAGRLNILRSLQIATGATTTPPSP
                     TAAPAATATAVPAPPKPTATPALLSLVLNPAHVLSGGTVTANGRGYQPGEAVGLRMTG
                     PEGRNHELGTAQVGADGSFQQAVTIPADLTPGTGMFFALGALSNALTSAPISIDTATQ
                     PGGPPGPPGPATGSRIEGTISGVPLDQVQVYLQVGNGVRDWRYGATDNLGFYHFDNLP
                     AGDYTVGLNGRNGLTVPAPVSLSVDGQPGTVRVINFDFGAGGSPPVAPPAEAPAPDPA
                     TAFAPAGDPARPGVVYFPPVQHTLRGVFLAYWQQHGGLPVFGYPLSEEFVEISDTDGK
                     PYRVQYFERNRFEYHPEYAGTPNEVLLGLLGRAVTLGRTFAPPIATLGDANHVYFPQT
                     GHMLGGAFLAYWRAHGGLAIFGYPISEEFAENGYTVQYFERNRFEFHPEY"
BASE COUNT          402 a          911 c         1071 g          387 t
ORIGIN      
        1 cgtactcggg gtggaactca aagcggttgc gctcgaagta ttggaccgta tagccgttct
       61 cggcgaactc ctcggagatg ggatagccga agatcgccag gccgccgtgc gcgcgccagt
      121 aggccaggaa ggccccgccc agcatgtggc ccgtctgcgg gaagtagacg tggttggcgt
      181 cgccgagcgt ggcgatgggc ggggcgaagg tgcggcccag ggtcacggcg cggcccagca
      241 ggcccagcag cacctcattg ggcgtgccgg catactcggg gtggtattca aagcggttgc
      301 gctcgaagta ttgcacacga tacggcttgc cgtcggtgtc cgaaatctcg acaaactctt
      361 ccgagagcgg atagccgaag acgggcaggc cgccgtgttg ctgccagtag gccaggaaca
      421 cgccgcgcag cgtgtgctgg acgggcggga agtagaccac gccgggccgg gcgggatcgc
      481 cggcgggggc aaacgcggtg gccgggtcgg gcgccggggc ttcggcgggc ggggccaccg
      541 gcggcgaccc accggccccg aagtcgaagt tgatcacccg cacggtgccc ggctgcccat
      601 cgacgctgag cgagaccggc gccggcaccg tcagcccgtt gcgcccgttg aggcccacgg
      661 tgtagtcgcc cgcgggcagg ttgtcgaagt gatagaagcc caggttatcg gtcgcgccgt
      721 agcgccagtc gcgcacgccg ttgcccacct gcagatagac ctgcacctgg tcgagcggca
      781 ccccgctgat ggtgccctcg atgcgcgacc cggtggccgg ccccggcggg ccggggggcc
      841 cgccgggttg cgtggccgtg tcgatgctga tcggggccga agtcagcgcg ttgctcagcg
      901 cacccagggc gaagaacatg cccgtgccgg gggtcagatc ggcggggatg gtcacggctt
      961 gctggaagct gccgtcggcg cccacctggg cggtgcccag ttcgtggttg cgcccttcgg
     1021 ggccggtcat gcgaaggccc acggcttcgc cgggctggta gccgcgcccg ttggccgtca
     1081 cggtgccgcc ggacaggaca tgggccgggt tcaggaccag cgagagcagg gccggcgtgg
     1141 cggtcggctt aggcggcgcg ggcacggcgg tcgcggtcgc cgccggggca gcggtgggtg
     1201 acgggggcgt ggtcgtcgcg ccggtggcga tctgcagcga gcgcaggata ttgaggcggc
     1261 ccgcgccgta atgcgggtcc cagcccgccg ggcccaggtc gtcggcgccg tcttccagca
     1321 ggcgctggag gtcgtcgggc gtgagatcgg gccgcgcggc cagcagcagc gccgccgcgc
     1381 cggtcacgag cggcgccgcc gacgaggtgc cgctggccca ggtgtagccg gcgcggccct
     1441 ggccccatcc ggtgcttacg atattcacgc ccggcgcggc caggtccaca aaggcgccgt
     1501 aggacgagaa ggcggcggtc ttgtcctgct cgtcggtggc gctcacggcc acgacctcgt
     1561 cgaagcccgc cgggtagcgg gccgcgccgt cgggtgtgtt gcccgccgag gccacgacca
     1621 gcacgccgtg gtcgcgggcg tagcgcacgg cttcctgcat ggacgacgcg ggatactcga
     1681 tcccggcgct gatgttgatg accttggcgc cgtggtccac cgcgaaatgg atgccgcgcg
     1741 agaagtcgcc cacgttgccg gcgccgtcct tgtcgaggat cttgaccggc atgatccgcg
     1801 tggcccaggc gaccccggcc acgccgatgt tgttgttgct gagcgcgccg accagcccgg
     1861 cggtataggt gccgtggccg ttatcgtccg cggcggtggt cgtatcggct acgaagttgt
     1921 agccgggcac cagcttgccc accaggtccg ggtggttggg ggcgatgccg gtatcgatca
     1981 tcgccacgat cacatccggg ctgccggtgg tgatgtccca ggcggcgggc gcgcccacct
     2041 ggttcaaggc ccactggcgg gaaaagagcg ggtcgctggg cacaaaggcg gcgtgcaggc
     2101 ggtaatccgg ctcggcgtag accacgccgg gcagcgcggc cagggcgcgc gccgcccccg
     2161 ccacatcgag ccccggctga tcggcgtgca accggtagag gccgcttccg gcatccagaa
     2221 cctcgactcg gttcaggccc caggcggcca gcgccgggcg caactccgcg ccaccggcct
     2281 ggatcgtccc ggcggcgtcg aggcgcaggc gctgggcggg cgccagttgg acgaggagct
     2341 gcccggcctg ccagtcggcg ggctgcgcgg gcggcggcac ggggcgcggt ttggcgaccg
     2401 cgcggcgatg cggcggggtc gcgggcccgg ccacgcccgg cgcgggggcc aggctcagca
     2461 cacaggcgag caacaggagc acaacggccg gcacgcgcac agccgggtgt cgggcgagcg
     2521 gggaggtgcg gtatgtagtt aacataggcg gtcctgggcg gaaacgaagg gcgcgccggc
     2581 caactgggca cccgcaacga taggtctatt ataccagcta ttggtacaac gcataaccat
     2641 ccggcagggt gcaaagctgt tcaatcgcac cgcagagacg cagaggacgc agagatcatt
     2701 acggtagggt ctccttacgc cccggctgcc cgcaggcggg gccgcagttc tgcgctgttg
     2761 ctccagcctg g
//
LOCUS       DASUYC010000286         2767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1673612, whole genome shotgun sequence.
ACCESSION   DASUYC010000286 DASUYC010000000
VERSION     DASUYC010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1673612"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VF276_04660"
     CDS             complement(<1..218)
                     /locus_tag="VF276_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6606188.1"
                     /translation="MTTKTSFKARPYGGEADLPAICDMLNACDAVDQNDDNYAVDDLR
                     LEFSDPHVDQARDLRVWEDATGRVVALG"
     gene            complement(671..>2767)
                     /gene="mfd"
                     /locus_tag="VF276_04665"
     CDS             complement(671..>2767)
                     /gene="mfd"
                     /locus_tag="VF276_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX6606189.1"
                     /translation="DLELFGRTQPQRRAGQKPTRTTAVEEFLREVKPGDFVVHIEHGI
                     ARYTGLVKMGTSGAEREYMQLEYAEGDRLYVPIEQTDRVAAYVGTGAGTPALHRLGTG
                     DWTRTKKRVQAATVELARELLALYASREAAPGYAYTPDSTWQEELEAAFPYIETADQM
                     RAIDEVKADMENARPMDRLICGDVGFGKTEVALRAAFKAVMDGKQVAVLVPTTILAQQ
                     HYNTFQSRMAAFPVTVEMLSRFRSPKAQKRILGELAVGGVDIIIGTHRLLQKDVQFKD
                     LGLVVVDEEQRFGVRHKERLKQLRQEVDVLTMTATPIPRTLHMAMVGVRDMSVIETPP
                     EERLPIKTYVTAYRDSLVREVIQREIERGGQVYFVHNRVQSIGIVLRELQKLLPDVRF
                     AVAHGQMDEDDLEDVMTAFIDQKYDVLICTTIIESGLDIPNVNTLIVDQANNYGLAQL
                     YQLRGRVGRGDKRGYAYFLYHQGRKVTDVAQERLQTIEQATELGAGFRIALKDMEIRG
                     VGNLLGPEQHGNVGAVGFDLYTRLLAGAVEEAKGHERPVEPPPVTFDLPITAYLPEDY
                     VPEASERLRLYQRLARGRSESGVRDMRRELEDRFGPLPQPAENLLLIVRLKSMALRAG
                     VEAINTIDDEFVIVLGAEAARRRLGPQLHYNLGVKLGDNVRVTQHQVRLQRRALGNDW
                     VTVLEDTLEELIEAAA"
BASE COUNT          422 a          892 c          934 g          519 t
ORIGIN      
        1 tggcccagag ccaccacccg gcccgtcgca tcttcccaga cgcgcaggtc gcgtgcctgg
       61 tccacgtgcg ggtcgctgaa ctccaaccgc aaatcgtcga cggcgtagtt gtcatcattc
      121 tgatcgacgg cgtcgcaggc gttaagcata tcgcaaatgg cgggcagatc ggcctcgccg
      181 ccgtagggcc gcgccttgaa actcgttttc gtggtcatgc ttgattctcc tgaatatcct
      241 gtcacacaag gcgtgtcact ctgagcgcag cgaagggtct cgtcttttag ccggcaagac
      301 ggagattctt cgctccgctc agaatgacac atcccgttga cagcttacta agttgcgctg
      361 atttttgcgt attgatgggc acaatgtagg gatcttacca cagcggggtg ccgggcggca
      421 tcggccctga aacggagacc ggctccgtct atcggcgggt aggaacgggg atggcggggg
      481 tccaggaacc gagccggatt acgtctggag actcagcggg gcggcgggcc ggtttcaaac
      541 cggcccctac ggcgaggatc gtgtccggcg gggcgggccg gtttgaaacc ggcccctaca
      601 gcgaggatcg tggcggagcg gcgggccggt ttgaaaccgg cccctacacc gcatcccgtc
      661 cagtctgaga ctaggcggcg gcctcgatca gttcttcgag cgtgtcttcc agcaccgtga
      721 cccagtcgtt gccgagggcg cggcgttgca ggcggacctg gtgctgggtg acgcggacgt
      781 tgtcgcccag cttgacgccg aggttgtagt gcagttgcgg ccccagacgg cggcgggcgg
      841 cctcggcgcc cagcacgatg acgaactcgt cgtcgatggt gttgatcgcc tcgacgcccg
      901 cgcgcagggc catgctcttg aggcggacaa tcagcagcag gttctcggcg ggttggggca
      961 gcgggccgaa gcggtcctcc agttcgcgac gcatatcgcg cacgccgctt tcgctgcgcc
     1021 cgcgggccaa ccgctggtag agccgcaagc gctcgctcgc ctcgggcacg taatcttcgg
     1081 gcaggtaggc ggtgatcggc agatcgaagg tgacgggcgg cggctcaacc gggcgctcgt
     1141 ggcccttggc ctcctcgacc gccccggcca gcagccgcgt gtagagatcg aagccgaccg
     1201 cgcccacgtt accgtgctgc tccggtccga gcaggttgcc cacgccgcgg atctccatgt
     1261 ctttcagggc gatgcggaag cccgcgccca actcggtcgc ctgctcgatg gtctgcaggc
     1321 gctcctgagc cacgtcggtc actttgcgcc cctggtgata gaggaaatag gcgtagccgc
     1381 gcttgtcgcc gcgacccacg cgcccgcgca actggtacaa ctgggccagc ccgtagttgt
     1441 tggcctggtc cacaatcagc gtgttgacgt ttgggatatc gagaccggac tcgatgatcg
     1501 tggtgcagat caacacgtcg tacttctggt cgatgaaggc agtcatcacg tcttcgagat
     1561 cgtcctcgtc catctggccg tgggccacgg cgaagcggac gtcgggcagc agcttttgca
     1621 actcgcgcag cacgatgccg atgctctgca cgcggttgtg cacgaaatag acctggccgc
     1681 cgcgctcgat ctcgcgctgg atcacctcgc gcaccagcga gtcgcggtag gccgtgacgt
     1741 aggttttgat cggcaggcgc tcttcgggcg gcgtctcgat cacgctcatg tcgcgcacgc
     1801 cgaccatcgc catgtgcagc gtgcgcggga tcggcgtggc ggtcatggtc agcacgtcga
     1861 cttcctggcg caactgcttg agccgctcct tgtggcgcac gccgaagcgc tgctcctcgt
     1921 ccacgaccac caggcccagg tccttgaact gcacgtcctt ttgcagcagg cggtgggtgc
     1981 cgatgatgat gtccacgccg ccaacggcca gttcgccgag gatgcgcttc tgcgccttgg
     2041 gcgagcggaa gcgcgagagc atctccactg tgactgggaa ggcggccatg cggctctgga
     2101 aggtgttgta gtgctgctgg gccaggatgg tcgtcggcac gagcacggcc acctgcttgc
     2161 cgtccatgac ggctttgaag gcggcccgta gcgcgacctc ggttttgccg aagcccacat
     2221 cgccacagat gagccggtcc atgggccggg cgttctccat gtcggccttg acctcgtcga
     2281 tggcgcgcat ctggtctgcc gtttcaatgt aggggaacgc cgcttccagt tcttcctgcc
     2341 aggtgctgtc cggagtatag gcgtagccgg gcgcggcttc gcgcgaggcg tagagcgcca
     2401 gcagttcgcg ggccagttcc accgtggccg cctgcacgcg ctttttcgtg cgcgtccagt
     2461 cgccggtgcc caggcggtgc agcgcggggg tgcccgcccc ggtgccgacg taagcggcca
     2521 cgcggtcggt ctgctcgatg ggcacgtaga gccggtcgcc ctcggcgtac tccaactgca
     2581 tgtactcgcg ctcggcgccg ctggtgccca tcttgaccag gccggtatag cgggcgatgc
     2641 cgtgctcgat gtggaccacg aagtcgcccg gcttgacctc gcgcagaaac tcctcgacgg
     2701 cggtggtgcg ggtcggcttc tgcccggcgc ggcgctgcgg ctgcgtgcgc ccaaacaact
     2761 ccaggtc
//
LOCUS       DASUYC010000287         2726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_16693,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000287 DASUYC010000000
VERSION     DASUYC010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16693"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2012
                     /locus_tag="VF276_04670"
     CDS             <1..2012
                     /locus_tag="VF276_04670"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEX6606190.1"
                     /translation="INAAWGLGEAIVGGQVTPDTVVVNKADGSVIEQQINEKDVMTVR
                     TPTGTEEAPVPPDRRQQPVLTTAQAGDLARLGTRIEALYGRPMDIEWARDDGQVFVVQ
                     ARPITALPEPGAVPAARAAEWELPHPKGRYMRSSLLELLPDPLSPLFATLGMAAFNRA
                     MSAFGRKLGLGALLTNDMLTTVNGYGYYDLTLTPVQAAATALGMVRFMGGAPRLLRTA
                     ETRWQAAHARYAEIVSRWEGADLAATPATTLLQGAQVITARAAQYYMSIQSGILPDAY
                     MTETLFSSVYDRFFKRDDDPAALTFMLGFDSAPIRAEKSLYDLAQWVREQPGLAATLA
                     GMSSEQFAAAYQDDAAAAAVPDPAWPEFRRRLGEHLARFGHAIYDLDFAKPLLADDPA
                     PVLETLKFFLSGQAPDPHKRQATAQAAREAAMRGIHRGRAGLSANVARRLLRTAQRFA
                     PLRENAIADAGLGWPVLRRMLRELGSRLVARHSLAAPDDVFWLTLADLQAAVAALDAG
                     QPTTDYQPVVAERRATWARRRAQTPPLALPLKGGARFLGVDFTSMLPARSNEAAGTTL
                     KGIAVSPGQVRGPARVIHGPEEFTQMRQGDILVARITTPAWTPLFALAAGVVTDIGGP
                     LSHGSIVAREYHIPAVLGTSTATERLHSGEQITVDGDAGTVTLTG"
     gene            complement(2169..2420)
                     /locus_tag="VF276_04675"
     CDS             complement(2169..2420)
                     /locus_tag="VF276_04675"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HEX6606191.1"
                     /translation="MSVQVSISAKVPEELRRQVDRLAAATHRNRSWVVAAALRAYLAR
                     ELEFVEAVEAGIQQREAGEVIPHTDVVAYVRDRRATETE"
BASE COUNT          412 a         1036 c          871 g          407 t
ORIGIN      
        1 tgatcaacgc ggcctggggc ctgggcgaag ccatcgtcgg cgggcaggtg acgcccgata
       61 ccgtcgtggt gaacaaggcc gacggcagcg tgatcgagca gcagatcaac gagaaagacg
      121 tgatgaccgt gcgcaccccg accggcacgg aagaagcgcc cgtcccgccg gatcgccgac
      181 aacagcccgt gctcaccacg gctcaggcgg gcgacctggc ccggctcggc acgcgcatcg
      241 aggccctgta tggccggccc atggacatcg aatgggcgcg cgacgacggc caggtcttcg
      301 tcgtgcaggc ccgcccgatt accgcgctgc ccgaaccggg cgccgtgccc gccgcccgcg
      361 ccgcggaatg ggaactgccc caccccaagg gccgctacat gcgttccagc ctgctggaac
      421 tgctgcccga cccgctgtcc ccgctgtttg ccacgcttgg gatggccgcc tttaaccggg
      481 ccatgagcgc attcggcagg aagctgggcc tcggcgccct gctgaccaac gacatgttga
      541 ctaccgtcaa cggctacggc tactatgatc tcacgctgac gccggtccag gcggcggcga
      601 cggccctcgg tatggtgcgc ttcatgggcg gcgccccgcg cctgttgcgc accgccgaga
      661 cccgctggca ggcggcgcac gcccgctatg ccgagatcgt cagccgctgg gaaggcgccg
      721 acctggccgc taccccggcg accacgctgc tgcaaggcgc gcaggtgatc accgcccgcg
      781 ccgcccagta ctacatgagc atccagagcg gcatcctgcc cgacgcctat atgaccgaaa
      841 cactcttctc cagtgtctat gaccggttct tcaagcgcga cgacgatccg gcggcgctga
      901 cgttcatgct cggcttcgac agcgcgccga tccgggccga aaaatcgctc tacgacctgg
      961 cccaatgggt gcgcgaacaa ccgggcctgg ccgccacgct ggccggcatg tcgagcgagc
     1021 agttcgccgc cgcctaccag gacgatgcgg ccgccgcagc ggtgcccgac ccggcgtggc
     1081 cggagttccg ccgccgcctg ggtgagcacc tggcccgctt cgggcacgcc atctacgacc
     1141 tcgactttgc caagcctttg ctcgccgacg atccggcgcc ggtgctggaa accctcaagt
     1201 tcttcctgag cggtcaggcg cccgatcccc acaagcgcca ggccacggcc caggcggcac
     1261 gcgaggcggc gatgcgcggc atccatcgcg ggcgagccgg cctctcagcc aatgtcgcgc
     1321 ggcgcctgct gcgcacagcc cagcgcttcg ccccgctgcg cgagaacgcc atcgccgacg
     1381 cgggcctggg ctggccggtg ctgcggcgca tgttgcgcga actcggcagt cgcctcgtcg
     1441 cccggcacag cctcgccgcc ccggacgacg tgttctggct cacgctggcc gacctccagg
     1501 cggccgtcgc cgccctggac gccgggcaac ccacgacgga ttatcagccc gtcgtggccg
     1561 agcgtcgcgc cacctgggca cggcggcgcg cccagacacc gccgctggcg ttgccgctca
     1621 agggcggcgc ccgcttcctg ggtgtggatt tcaccagcat gctgcccgcg cgcagcaacg
     1681 aggcggcggg caccacgctg aaggggatcg ccgtcagccc cggtcaggtc cgcggcccgg
     1741 cacgggtcat ccacggcccg gaggagttca cgcagatgcg ccagggcgac atcctggtcg
     1801 cgcgcatcac cacgcccgcc tggacgccgc tcttcgccct ggccgcgggc gtcgtcaccg
     1861 acatcggcgg cccgctcagt cacggctcga ttgtcgcccg cgagtaccac atcccggcgg
     1921 tgctgggcac gagcacggct accgagcgcc tgcacagcgg cgaacagatt acggtcgacg
     1981 gcgacgccgg caccgtcacg ctcacgggct aggctacacc gagggtggcg gcggttatcg
     2041 cgccgccacc ccccgtccat gggtggaacc ggccctccgc ctgcgcgatg tagaggccgg
     2101 tttgaaaccg gcccgccgtc cggtctgatc ggcgctaccg gtttaaggct ggggtcgtcc
     2161 tcattggatt actcagtctc cgtcgcccgc cggtcgcgga cataggccac cacgtcggta
     2221 tgaggtataa cctcacctgc ttcccgctgc tggatgcctg cttccaccgc ctcgacaaac
     2281 tccagttccc gcgccaggta ggcccgtagc gctgcggcca cgacccacga gcgattgcgg
     2341 tgcgtagcag cggcaagacg gtctacctgt ctcctgagtt cctccggcac cttcgccgag
     2401 atgctgacct gcacgctcat attgcatcct cctgcgatgt gttttcttca ggtccctggt
     2461 attcaaggta cacgattctc accgtaagtc aataccgctt tcccgtcgta ggagcggacc
     2521 ggcgtgtccg ccctgccgcc gcgcgaaccc actccgccgt ccccctgatc tccgccgggc
     2581 cagcccgcgc aggcgggctt cgtaaccgtt gcccgcccgt tcacgggccg ggcgccgccc
     2641 ccggttgcgc gccccggcgg gctgtgctat gataggcgca gaacgatccc catcccccaa
     2701 cgacctcgcc cgaccgcaac agccga
//
LOCUS       DASUYC010000288         4189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_247765,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000288 DASUYC010000000
VERSION     DASUYC010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247765"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..1118
                     /locus_tag="VF276_04680"
     CDS             147..1118
                     /locus_tag="VF276_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015121894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6606192.1"
                     /translation="MNTADTMVIRTQDLRKTFKGVQALNGLTLNVPKNSIFGFLGPNG
                     AGKSTTIKLLLGLIRPTGGSAAVFGQDITHDSVSIRQRVGYLAQDPRYYEHLTARETL
                     RYTAHFFYSGPPHLIEDRVTEMLDLVGLADKADRPIKGFSGGERQRLGIAQAQVNYPD
                     LLILDEPAASLDPQGRHDVLAVMEKLRKYTTIFYSTHILEDVQRVSDTVAILNRGQLI
                     AEAPIAELLAGTGGTTIYTLTVKGDPGTLATRVRSQPWVQDLHGAADDGLTTWQVSVS
                     DPDAAEAQLLRLVLTDPAVTVTAFGRKVYNLEEVFLSLVEGSNQNGR"
     gene            1108..1968
                     /locus_tag="VF276_04685"
     CDS             1108..1968
                     /locus_tag="VF276_04685"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX6606193.1"
                     /translation="MAANTTLQQDAAAPGRAALQPVQGSPWLGGFRNMLSKELGDWFG
                     TRRWLIQTGIWVAIINGFVAFILFLVPTIQQEAAQSGAAPPPEDTDPLLLGLSLFFTL
                     AGAAGVIGTIILAQDEIIGEKQSGTAAWILSKPVARAAFILSKLAASSIGIGIFIIGI
                     PAVICYTQVSLAVGHPIDLPSYLAGLVPLALTLLFYLTLTLLLGVVFDQRGPLLGIAF
                     GLFFGASILVQFIPELAQVLPINLPNAGSALAEGRGLPDSAVLPLILTPIWSFLFIVA
                     ALWRFQRMEI"
     gene            2244..3362
                     /gene="apbC"
                     /locus_tag="VF276_04690"
     CDS             2244..3362
                     /gene="apbC"
                     /locus_tag="VF276_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEX6606194.1"
                     /translation="MAVTTVSEEQVLNALRTVKDPDLHKDLVTLKFIEDLKIEGGAVS
                     FTIVLTTPACPVKDLLKQQSEDAVSAVPGVTSVTAAVTSRVQGSYVPQREGIPGVRNI
                     IAVGSGKGGVGKSTVSVNLALALAQTGARVGILDADIYGPNVPLMMGAVGKPETSPSG
                     KIQPMRTYNIDMISIAFFLPDDNSPVIWRGPMLTKLLNQFMYDVEWGELDYLIMDMPP
                     GTGDVQLTTAQSLPLTGAVIVSTPQDVALMDAKRALTMFQKLNVPILGMVENMSTFIC
                     PHCEQETDIFGHGGAHATAQRLGIPFLGDIPLKLEIRTGSDDGRPIVVDQPQAKEAQA
                     FRTVAKALAAQVSILAYERTGSASRGQGAPLPLFRRRG"
     gene            complement(3417..>4189)
                     /gene="hemG"
                     /locus_tag="VF276_04695"
     CDS             complement(3417..>4189)
                     /gene="hemG"
                     /locus_tag="VF276_04695"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HEX6606195.1"
                     /translation="MFLALRDGMAELPAALAAHLGPAVVRTGVEVVRMVRTGAGDDSG
                     YRLHLSSGEQITARQVVLATPAPATARLVAAEAPDLAAALGAIRYVSSATISLGYRAA
                     DLPRPLDGFGFVVAAREPTRLLACTWSSTKFDARAPQDHVLLRAFVGGAHHEELVDLP
                     DAALLALVYEELRAILGITAAPVVSRIFRWPKANPQYDVGHLERVARIESLAAAVPGL
                     YLTGSAYRGVGIPDCVRGAKATAMRVAAALHAQAPAAI"
BASE COUNT          709 a         1395 c         1368 g          717 t
ORIGIN      
        1 agcgtccagt actgccgcta aaggacgcgc ccaacggttg caatgcgaac ccaatacacc
       61 atcttcgctc acgatgcagg catgtgccgg cctgcccgta gcgcttgatc ggggctatgg
      121 ctcatcatat gaggagggaa ctgcccgtga acaccgccga cacaatggtg atccgcaccc
      181 aggatttgcg taagaccttt aagggcgtgc aagcgctcaa cggcctgacg ctgaacgtcc
      241 cgaaaaactc gattttcggc tttctcggac ccaacggcgc gggcaaaagc accacgatca
      301 agctcttgct cggcttgatc cgaccgacgg gcggcagcgc cgccgtgttc ggtcaggata
      361 tcacccacga cagcgtctcc attcgccagc gtgtcggcta cctggcccag gatccgcgct
      421 attacgagca cctgactgct cgcgagacgc tacgctatac cgctcacttc ttctacagcg
      481 gccctccaca cctgatcgaa gaccgggtca cggagatgct cgatttggtg ggcctggccg
      541 acaaagccga tcgcccgatc aagggcttct ccggcggtga gcgccagcgc ctcggcatcg
      601 cccaggcgca ggtcaactac cccgacctgc tgatcctcga cgaacccgcc gcatcgctcg
      661 acccgcaggg ccgccacgat gtgctcgcgg ttatggagaa gttgcggaaa tacaccacca
      721 tcttctactc gacccatatc ctggaggatg tgcagcgggt cagcgacacc gtggcgatcc
      781 ttaaccgcgg gcaactgatc gccgaggcgc ccattgccga actgctggcg ggcaccggcg
      841 gcacgaccat ctatacactc actgtgaagg gcgaccccgg caccctcgca acccgcgtgc
      901 ggagccagcc ctgggtccag gatctgcacg gcgccgcgga tgacggcctg accacctggc
      961 aggtgagtgt gagcgatccg gatgccgcgg aggcccaact gctacggctc gtgttgaccg
     1021 atcccgccgt gaccgtgacc gccttcgggc gcaaagtcta caacctggaa gaagtattcc
     1081 tcagcctggt ggaagggagc aaccaaaatg gccgctaaca caactttgca gcaagacgcc
     1141 gccgcgccgg gccgggcggc gctccagccg gtccagggca gcccctggtt ggggggcttc
     1201 cgcaacatgc tcagcaagga actgggcgac tggttcggca cccgccgctg gctcatccag
     1261 actgggatct gggtggcgat catcaacggc ttcgtggcct ttatcctgtt cctggtgccg
     1321 acgatccagc aggaggcggc ccagtccggc gcggctcctc cccccgaaga caccgatcct
     1381 ctgctgctgg gtctgagtct cttcttcaca ttagccgggg ccgccggagt catcgggacg
     1441 atcatcctgg cccaggatga gatcatcggc gagaagcaat cgggcacggc ggcgtggatc
     1501 ctctccaaac cggtggcgcg cgccgcgttc atcctgtcca agctggcggc aagcagcatt
     1561 ggcatcggga tcttcatcat cgggataccg gcggtgatct gttataccca ggtctcgctg
     1621 gcggtgggtc accccatcga tctgccgtcc tatctggcgg gcctggtacc actggcgctc
     1681 acgctgcttt tctatctcac cctgaccctg ctcctcggcg ttgtgttcga tcagcgcggg
     1741 ccgttgctcg gcattgcctt tggcctgttt ttcggcgcgt ccatccttgt ccagtttatc
     1801 ccggagttgg cccaggtgct ccccatcaac ctgcccaatg ccggcagtgc gctggccgaa
     1861 ggcagagggt tgccggatag cgccgtcctc ccgctgatcc tcacaccgat ctggagcttc
     1921 ctgtttatag tggcggccct gtggcggttc cagcggatgg agatttagcg cccaatagct
     1981 tgcctgggcg gggggcccgc gctttgggcg tggcttcttt caggtggcgg gtttccggcg
     2041 gggagggcct ggcgggcctg ctgctgcctc ctaatcacgt tgacagaccc ccctgcttgg
     2101 ggtatagtta ggtgatgtat tgtgcgctac tgcgtattgc tttttaagtg tgctaagatg
     2161 atcgataatg atctgcgttt cccgcgcacc cggagggggt aggtcctgac cggcgcgggc
     2221 tcgtgaatgg gagaaggtat acgatggcgg ttacgaccgt ttctgaggag caggtcctga
     2281 atgccctgcg gacggtgaaa gatcccgatc tgcataagga cctggtcacg ctcaagttta
     2341 tcgaagacct caagatcgaa ggcggggccg tcagcttcac cattgtgctg actacgcccg
     2401 cctgcccggt gaaggacctg ctcaagcagc agtccgaaga cgcggtgagc gccgtccccg
     2461 gcgtcacctc tgtcaccgcc gccgtgacct cgcgcgtgca gggatcgtat gtgccgcagc
     2521 gcgaaggcat ccccggtgtg cgcaacatca tcgcggtggg cagcggcaag ggcggcgtgg
     2581 gcaagtccac cgtctcggtc aacctggccc tggccctggc ccagaccggc gccagggtgg
     2641 gcatcctcga cgccgatatc tacggcccga atgtgccgct gatgatgggc gcggtgggca
     2701 agcccgagac cagccccagc ggcaagatcc agccgatgcg gacgtacaac atcgacatga
     2761 tctcgatcgc cttcttcctg ccggatgaca actcgccggt gatctggcgc ggaccgatgc
     2821 tgaccaagct gctcaaccag ttcatgtacg acgtggagtg gggcgagctg gactatctga
     2881 tcatggacat gccgcccggc accggcgacg tgcagttgac caccgcgcag tcgcttccgc
     2941 tgacgggcgc cgtgatcgtg agtacgccgc aggacgtggc cctcatggac gctaagcgcg
     3001 cgctgaccat gttccagaag ctgaacgtgc cgatcctggg catggtcgag aacatgagca
     3061 cgttcatctg tccgcattgc gagcaggaga ccgacatctt cggccacggt ggggcgcacg
     3121 ccacggcgca gcgcctgggc atccccttcc tgggcgatat cccgttgaag ctggagatcc
     3181 gcacaggcag cgacgacggc cgcccgatcg tggtggatca gccgcaggcg aaggaagccc
     3241 aggctttccg caccgtcgcc aaggcgctgg ccgcgcaggt cagcatcctg gcctacgagc
     3301 gcacgggcag cgcgagccgg ggccagggcg ccccgctgcc gctcttccgc cgccgcggct
     3361 aagcgcccca aaccagcaca ccagaccggc gccgggacat tcccggcgcc ggtcttttag
     3421 atcgccgcag gggcctgcgc gtgcagcgcg gcggctaccc gcatggccgt cgccttcgcg
     3481 ccgcgcacgc agtcggggat gccgacgccg cggtaggcgc tgccggtcag gtagaggccg
     3541 ggcactgcgg cggccaggct ttcgatgcgc gccacgcgct ccaggtggcc cacgtcgtac
     3601 tgcgggttgg ccttcggcca gcggaagatc cggctcacca ccggcgcggc ggtgatgccc
     3661 aggatggcgc gcagctcctc gtacaccagg gccagcagcg cggcgtccgg caagtccacc
     3721 aactcctcat gatgggcgcc gcccacgaag gcgcgtagga gcacgtgatc ctggggcgcg
     3781 cgggcgtcga atttggtcga actccaggtg caggccagca ggcgcgtcgg ctcgcgggcg
     3841 gcgaccacga agccgaagcc gtcgagcggg cggggcagat cggcggcgcg gtagcccagc
     3901 gagatggtcg cgctggagac atagcggatc gcgccgaggg ccgcggcgag gtcgggtgcc
     3961 tcggcggcca ccagacgggc ggtggcggga gccggcgtgg caaggaccac ctgccgggcc
     4021 gtgatctgct ccccgctgct caggtgcagg cggtagccgc tgtcgtcgcc ggcccctgtc
     4081 cgcaccatgc ggacaacctc cacgcccgtg cgcaccaccg ccggccccag gtgggcggcc
     4141 agcgccgcgg gcagttcggc catgccgtcg cgcagggcca ggaacatgg
//
LOCUS       DASUYC010000289         2925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_732775,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000289 DASUYC010000000
VERSION     DASUYC010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732775"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VF276_04700"
     CDS             <1..471
                     /locus_tag="VF276_04700"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEX6606196.1"
                     /translation="NFYYFSGGDPQYLAATLAGTPTWDVIRRRHEAGAVLAGCSAGAM
                     AFGGWIPNLRAAFTGRATDWHPALGLVPPLLVLPHFDRMANFIGRAVLRRMVQTAPAG
                     ACVIGVDEDTALVRFPAGDAWQVWGRQTVSVFDGPGNGGATVYQSGQTVVLPGV"
     gene            496..1215
                     /locus_tag="VF276_04705"
     CDS             496..1215
                     /locus_tag="VF276_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEX6606197.1"
                     /translation="MEPDLSAVRALTFDVFGTVVDWRGTIIREGRRLARAKGIQVDWP
                     AFADAWRGGYRPAMDRVRRGEVPWTNLDALHRLILDSLLDRFRLGGLDAAERDELNRV
                     WHRLRPWPDAVPGLRRLRRRFVTAALSNGNLALLTNMARHAGLPWDCILSADIAQHYK
                     PDPEVYETAVRLLDLPPAQIPMVAAHPDDLQAAARAGMRTAFVPRPQEWGLGRAADVP
                     PGVTFDLTATDFGDLAAKLGA"
     gene            complement(1266..>2925)
                     /locus_tag="VF276_04710"
     CDS             complement(1266..>2925)
                     /locus_tag="VF276_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606198.1"
                     /translation="SEIGSVYAMALDAAGNGWAWASPKPQTFATSFLLRIENGAWRVA
                     ATSTDNPALLPPEVFIQRMAITADGGDGWAIGHVGNSYPQPPMLWRLQQGAWRLARNS
                     FTPTFQMTDLALSADGRDGWITAQETKNEQDYRLLRLRNGSWDFAPQPTEGVLDYVAM
                     SADGRSGWGIGPSVVTPRGNEGAFRWNGTRWIAIHDEAVPLGEVAVRVAADNAGNGWL
                     ITLPVHEGEADGSVVRLRPDKAPRPAPLNLHLPGADADRVLRLRDVVVDGLGRGWVTG
                     SFDLGDNGELPTPKELYQPELLRLDGDNVTVVPIAGQPILQDKDNDPVSLAISPNGGH
                     AWLGGRNGFAQGRLGELREPWTHAKPAQTAPLPGAAICFAEVPYCLRGTFARYWTSHG
                     GLGQFGFPITPEVQETIGGKTYTVQYTQRNRFEYHPEYAGTPSEVLLGLLGNALADPR
                     KNEPPFQPAPQVAGSRWFPQTGHNIAAPFLAFWQANGGLPVFGLPRSEAFIEGNAADG
                     KPYLVQYFERNRLEYHPENKGTPYEMLLGLLGVEQFSKTYGFKP"
BASE COUNT          372 a          996 c         1099 g          458 t
ORIGIN      
        1 aacttctact acttctccgg cggcgatccc cagtacctgg ccgccacgct ggccgggacg
       61 cccacctggg atgtcatccg ccggcgccac gaggccgggg cggtgctggc cgggtgcagc
      121 gcgggggcca tggcctttgg cggctggatc cccaacctgc gcgccgcctt caccgggcgg
      181 gcgaccgact ggcacccggc gctcggcctc gtcccgccgt tgctggtgct gccgcatttc
      241 gaccgcatgg cgaatttcat cgggcgggcg gtgctgcgac gtatggtgca aaccgcgccc
      301 gccggggcct gcgtcatcgg cgtggacgag gacacggccc tggtgcgctt cccggcgggg
      361 gacgcgtggc aggtctgggg ccggcagacg gtgtcggtgt tcgacggccc tggcaacggc
      421 ggcgcgaccg tgtaccaaag cgggcagacg gtcgtcttgc cgggcgtctg atcacgccgg
      481 gagggggtgt tagtgatgga gccggatcta tcggcggtgc gggcgctcac gttcgacgtg
      541 ttcggcaccg tggtcgactg gcggggaacg atcatccgcg aggggcgccg gcttgcgcgg
      601 gcgaagggca tccaggtgga ttggccggcg ttcgccgacg cctggcgcgg cggctaccgg
      661 cccgcgatgg accgcgtgcg ccggggcgag gtgccctgga ccaacctcga cgcgctgcat
      721 cggctgatcc tcgactcgct gctggaccgc ttccgcctgg gcgggctgga cgccgccgag
      781 cgcgacgagc taaaccgggt gtggcaccgc ctgcgcccct ggccggacgc cgtgccgggc
      841 ctgcgccggt tgcggcggcg cttcgtcacg gcggcgctct cgaacggcaa cctggccctg
      901 ctcaccaaca tggcgcgcca cgccggcctg ccgtgggatt gcatcctctc cgccgacatc
      961 gctcagcact acaagcccga tccggaggtc tatgaaaccg ccgtgaggtt gctcgacctg
     1021 cccccggcgc agatcccgat ggttgccgcg caccccgacg acctgcaggc ggcggcgcgg
     1081 gccgggatgc ggacggcgtt tgtgccgcgc ccgcaggagt ggggcctggg ccgcgccgcc
     1141 gacgtgccgc cgggggtgac attcgacctc acggcgaccg acttcggcga tctggcggcg
     1201 aagctgggtg cctgaagcgg ggagggcggg ccgcggatca tggatgtccg cggcccgccc
     1261 tgctgttagg gcttgaagcc gtaggtcttg ctgaattgtt cgacgccgag caggcccagc
     1321 agcatctcgt agggtgtgcc cttgttctcc gggtggtact ccaggcggtt gcgctcgaaa
     1381 tactgcacca ggtagggctt gccgtcggcg gcgttgcctt cgatgaaggc ttccgagcgc
     1441 ggcaggccga agaccggcag cccgccgttg gcctgccaga aggccaggaa gggcgcggcg
     1501 atgttgtggc cggtctgcgg gaaccagcgg ctgcccgcca cctgcggggc cggctggaac
     1561 ggcggctcgt tcttgcgcgg gtcggccagg gcgttgccca gcaggcccag cagcacctcg
     1621 ctgggggtgc ccgcgtactc cgggtgatac tcgaagcggt tgcgctgcgt gtactgcacg
     1681 gtgtaggtct tgccgccgat ggtctcctgg acttcgggcg tgatcgggaa gccgaactgg
     1741 ccgaggccgc cgtggctggt ccagtagcgg gcgaaggtgc cgcgcaggca gtagggcact
     1801 tcggcgaagc agatcgccgc acccggcagc ggggccgttt gcgcgggctt agcatgggtc
     1861 cacggctcgc gcaactcgcc gaggcggccc tgggcgaacc cgttgcgccc gcccagccag
     1921 gcatgcccgc cgttcgggct gatcgccagc gacaccgggt cgttgtcctt gtcctgcagg
     1981 atcggctgcc cggcgatggg caccaccgtc acgttgtcgc cgtcgagccg cagcaattcg
     2041 ggttggtaga gctctttcgg agtgggcagt tcgccgttgt cgcccaggtc gaacgagccc
     2101 gtaacccagc cacgccccaa cccgtccacc acgacatcgc gcaggcgcag cacccggtcg
     2161 gcgtcggcgc cgggcaggtg gaggttgagc ggggcgggac gcggggcctt gtcggggcgc
     2221 aggcgcacga cgctgccgtc ggcctcgccc tcgtgaaccg gcagcgtgat cagccagccg
     2281 ttgcccgcgt tgtcggcggc cacgcgcacg gccacctcgc cgagcggcac cgcctcatcg
     2341 tggatcgcga tccagcgcgt gccgttccag cggaaggcgc cctcgttacc gcgcggcgtc
     2401 accacgctgg ggccgatgcc ccaaccgctg cgcccgtcgg cgctcatggc gacgtaatcg
     2461 agcacgccct cggtcggctg cggcgcgaag tcccaactac cgttgcgcag gcgcagcagg
     2521 cggtagtcct gctcgttttt ggtctcctgg gcggtgatcc agccgtcgcg cccgtcggcg
     2581 ctcagcgcca ggtcggtcat ctggaaggtg ggcgtgaagc tgttgcgggc caggcgccag
     2641 gcgccctgct gcaaccgcca cagcatcggc ggttgcgggt aggagttgcc gacgtggccg
     2701 atggcccagc cgtcgccgcc gtcggcggtg atcgccatgc gctggatgaa gacctccggc
     2761 ggcagcagcg cggggttatc ggtgctggtg gcggcgacgc gccaggcgcc gttctcgatg
     2821 cgcagtagga agctggtggc gaaggtctgc ggcttgggcg aggcccaggc ccagccattc
     2881 cccgcggcgt cgagggccat ggcgtacacg ctgccgattt cggag
//
LOCUS       DASUYC010000290         2699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1316791, whole genome shotgun sequence.
ACCESSION   DASUYC010000290 DASUYC010000000
VERSION     DASUYC010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1316791"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VF276_04715"
     CDS             <1..403
                     /locus_tag="VF276_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606199.1"
                     /translation="PAALFWAVAEGLRMGRFWLFGTAAWAVFGYVLIFVPPPVAGLTA
                     APWIAGFLVLLFAALLAGLTLPVYAVGWVMFTHRVHRQDLRRAVRQAGLLALFVVACL
                     GMALFSVFNGLNALLLFVVLALAEFFFLSRG"
     gene            433..1497
                     /locus_tag="VF276_04720"
     CDS             433..1497
                     /locus_tag="VF276_04720"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEX6606200.1"
                     /translation="MAQITIIGLGTIGSSLGLALHQYMETTEGRANAFTIVGYDPARD
                     AQMAQKSGSVDRVASDLPSAVRESPFVIIATPTLQVREILQTIAPLLPAGATVTDTAP
                     GKRLVLQWAAELLPPTISFVGGHPLPHRVPAPAVTRDEVEDEPPAADLFAGAPYCIMP
                     LPNAAEAAVNQVIGFAEAVGAKPYFTDPLEHDSFEAAVHDLPVLTSFALMRVLSESPA
                     WRDMGPLAGAAFREASRMASSDPLAVRAELVANRDNLLNWIDRYEATLQDLRELLAAT
                     DPADLEGAGSRDFGAALIAGRNARTGWLNPNAALTPAERQARDTMRQAGSGVLRSFFG
                     GFVADRLPGNRDPEHKDRRS"
     gene            complement(1859..>2699)
                     /locus_tag="VF276_04725"
     CDS             complement(1859..>2699)
                     /locus_tag="VF276_04725"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX6606201.1"
                     /translation="AWGIDYPALQALNPGLVYCTITGYGPVGPLAAHPGYDFAVQAAS
                     GLMSITGAPDGEPMKLGVAIVDLLTGLFAANAIQAALLGRARQPGRGGTHIEVSLLES
                     ALAALINVGQNVLVSGRDAGRYGNAHPNIVPYQLFAAADGWFALAVGNDRQFAALAAA
                     LGRPEWPGDPRFATNPARLAHREMLIPALEAIFEMRPAAHWQALLEAADIPVAPVQTV
                     AEALTSPQARALGIVREVDHPTAGRIRLVGAPLRFDGALPPIHRPPPLLGQHTAEILA
                     ELG"
BASE COUNT          365 a          955 c          962 g          417 t
ORIGIN      
        1 accggcggcg ctcttctggg ccgtggccga ggggctacgc atgggccgct tctggctgtt
       61 cggcacggcg gcctgggccg tcttcggcta cgtgctcatc tttgtgccgc cgcccgtggc
      121 ggggctgacc gcggcgccgt ggatcgccgg gttcctggtc ctgctgtttg ccgccctgct
      181 ggccgggctg accctgccgg tctatgcggt gggctgggtg atgttcaccc accgcgtcca
      241 ccgccaggat ctgcgccgcg cggtgcgcca ggccgggctg ctggcgctgt ttgtcgtcgc
      301 gtgcctgggc atggctctgt tttcggtgtt taacgggctg aacgcgctgc tgctgttcgt
      361 cgtactggca ttggccgagt ttttctttct ctcgcgcggc tagggcgggc cccggccgga
      421 aaggacgttc caatggcgca aatcaccatc atcggcctgg gcacgatcgg gagctcgctg
      481 ggcctggccc tgcatcagta catggagacg accgaggggc gggccaatgc gttcaccatc
      541 gtcggctacg accccgcgcg cgacgcgcag atggcccaga agagcggcag cgtggaccgc
      601 gttgcgtccg atctgccgtc ggcggtgcgc gagtcgccct tcgtgattat cgccacgccc
      661 acgctgcagg tgcgcgagat cctgcagacc atcgcgccgc tgctgccggc gggcgccacc
      721 gtgaccgata ccgcgcccgg caagcgcctg gtgctgcaat gggccgccga actgctgccg
      781 cccacgatca gcttcgtcgg cgggcacccg ctgccgcacc gcgtgcccgc gcccgccgtc
      841 acccgcgacg aagtcgagga cgagccgccc gccgccgatc tgttcgccgg ggcgccctac
      901 tgcattatgc cgctgcctaa cgcggcggag gcggcggtga accaggtgat cggctttgcg
      961 gaggccgtcg gcgcgaagcc gtattttacc gacccgctgg aacacgacag cttcgaggcc
     1021 gcagtccacg acctgccggt gctgaccagc ttcgccctga tgcgcgtgct gagcgaaagt
     1081 ccggcctggc gcgacatggg gccgctggcg ggcgccgcct tccgcgaagc cagccgcatg
     1141 gcctccagcg acccgctggc cgtacgcgcc gaactcgtcg ccaaccgcga caacctgctg
     1201 aactggatcg accgctacga ggcgacgttg caggacctgc gcgagttgct cgccgcgacg
     1261 gatcccgccg acctggaggg cgcgggcagc cgggacttcg gcgccgcgct gatcgctggg
     1321 cgcaacgcgc gcaccggctg gctcaacccc aacgccgcgc tcacgcccgc cgagcgccag
     1381 gctcgtgaca ccatgcgcca ggcgggcagc ggggtgctgc gcagcttctt cggcggcttc
     1441 gtcgccgacc gtctccccgg caaccgcgac cccgagcaca aggaccggcg ttcgtagtcg
     1501 atccaacgca cagtctatgt aggggccgtt ttcaaaccgg cccgcgccca tttccttgat
     1561 atgatgccgg ggaggtaggg cggcaggcgg gtttgaaacc cgcccctaca ccggcggggg
     1621 aggccggtag cgggccgggg gcgtggggca aggcgcggtc ggtttgatga ccaatgatgt
     1681 atatccatgt aggggccggt ttcaaaccgg cccgcgccgc acccgcattg taggggccgg
     1741 tttcaaaccg gcccgcgccg cccttcccgg cttgccaccc caccggcgtt gtaggggccg
     1801 gtttgaaacc ggcccgcgtc atgactgggg tggattaagg ggtcgtggta cgccgggttt
     1861 aacccagttc ggccaggatc tcggcggtgt gctggccgag caggggcggc gggcggtgga
     1921 tcggcggcag ggcgccgtcg aagcgcaacg gcgcaccgac cagccggatg cggcccgccg
     1981 tcgggtgatc cacttcgcgc acgatgccga gggcgcgcgc ctgcgggctg gtcagcgcct
     2041 cggccaccgt ctgcaccggg gccaccggga tgtcggcggc ttccagcagc gcctgccagt
     2101 gggccgcggg ccgcatctcg aagatcgctt ccagcgcggg gatcagcatc tcgcggtggg
     2161 cgaggcgggc ggggttcgtg gcgaagcggg gatcgcccgg ccactccgga cggcccaggg
     2221 cggcggccag ggccgcgaac tggcggtcgt tgcccaccgc cagcgcaaac cagccgtcgg
     2281 cggcggcgaa caactggtag ggcacgatgt tcgggtgggc gttgccgtag cggcccgcgt
     2341 cccgcccgct gacgagcaca ttctgcccca cgttgatcag cgccgccagc gccgactcca
     2401 gcagcgacac ctcgatgtgt gtgcccccgc gccccggctg ccgtgcgcgc cccagcagcg
     2461 ccgcctggat cgcgttggcg gcgaacagcc ccgtcagcag atcgacaatc gccacgccca
     2521 gcttcatcgg ctcgccgtcc ggcgccccgg tgatgctcat cagcccgctc gccgcctgga
     2581 ccgcgaaatc atagccgggg tgcgccgcca gcggccccac cggaccgtag ccggtaatgg
     2641 tgcaatagac cagaccggga ttgagcgcct ggagcgcggg gtagtcgatg ccccaggcg
//
LOCUS       DASUYC010000291         6110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_508399,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000291 DASUYC010000000
VERSION     DASUYC010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6110
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508399"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..1405)
                     /locus_tag="VF276_04730"
     CDS             complement(125..1405)
                     /locus_tag="VF276_04730"
                     /inference="COORDINATES: protein motif:HMM:NF018448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEX6606202.1"
                     /translation="MTAVESHPAAGRSHVQQVLAVAVQAGAIMLSSGAETARVEDTVN
                     ILARAFGIAYSQCLVMTTGIYVSLDDPQMAHPVTLVHRVPSRTLHYARVSAVNDLSRR
                     VAQGRVSVAEAARELDAIARAPDPYTLPVWLAGGAASAAGVALLLGGGPLDVVPAFVS
                     TVPVLLIGVLLQRSKIPALFSDALGAALATAVALALVSLGVPIHADMVIAGGIMTLVP
                     GAALLISVQDAISGNLLSAAARGLETLLKGAALACGVGVGLAGALNLGQQISVQSSTG
                     QVWQMPIQVVAAFAASACYAVLNHVPRFALGTAGVAGAVGWLAYLLLLQVNAGPLAAT
                     FLAAFVVGVLSWGFARGQHAPVSLFILPGILPLLPGVTIYNGMLELATNQSLQGLLLL
                     VHAVFLGGALAAGVGLSDSVAPHLWRRRLTRRRP"
     gene            complement(1835..3208)
                     /locus_tag="VF276_04735"
     CDS             complement(1835..3208)
                     /locus_tag="VF276_04735"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX6606203.1"
                     /translation="MALRTRGRVPAALLLGALCLSACSGNTLPAPGAGFAPPAVAPAA
                     TTAPGAVPFDPAAYRKNAVEPGAHLRVSSWGDTSEQQVVRDALARFQQVYPGITITYE
                     PQPSDYGTKLLAQVKAGAQPDVFYLDVDLPYQLIPHQVLLDLRPALTEVGRSKDDYFP
                     ALTGVFLGPDGQVYGLPKDFTSMALFYNTDLVTTPPKPGWTQADFTAWVKANTTGSGQ
                     TRVFGFASDLVFFLYWGNFALANGARVLDNNRCAINSPAGVTTLDWFYGLYQDHVLAL
                     SADVSAGWEGEAFAKQRAASIVVGGWINPYLTDPKAAFGVHYDAVPLPLGQGGARATT
                     LGFAGWGAAAQTRFPRAAAALVLFLTSRENEGAILQTGFALPSLKGMETHPFFQGSST
                     LSRISRLLYEGASYGQPGVWGGPANPQIQQALNEATERVFAGMQTSRQALDQACQEID
                     AALAAVQ"
     gene            complement(3391..4470)
                     /locus_tag="VF276_04740"
     CDS             complement(3391..4470)
                     /locus_tag="VF276_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX6606204.1"
                     /translation="MRIAVVGLHFGAEFVPIYQQHPDVERVGICDLDAELTARIGDQF
                     GVADRFAGLDEILADARYDAVHLLTPVPLHVEQTLAVLGAGKHCACAVPMATSLEDIQ
                     RVIAAQQASGKTYMLMETGAYTREFLYVEDLYRRGDLGTLTFLRGTYYQDLEGDFPRY
                     WRAQPPMHYLTHALAPLLALTRTRVEKVICLGSGRLRPDIQQPGGNMFPLQTGLFRLA
                     NTNLAAEVTRSWFQVAHPYTETFSVYGDKRGFEWLRENEAPLLFTLEPVVAGQRGRPV
                     TTAQIEVPDWGHLLPPAIAGFTRGHHGGSHPHLVHEFVRSIVEGRAPRIDSYTAADWT
                     APGICAHESAVQDGEPVLVPAFRGA"
     gene            complement(4977..>6110)
                     /locus_tag="VF276_04745"
     CDS             complement(4977..>6110)
                     /locus_tag="VF276_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606205.1"
                     /translation="TQIRAMRGAVGLMLRDWLAQANVPFHLGEPLQGALLLLAGLGFA
                     AGVRGARAERHGALICGSLLLLAPAELAVLSTTNTLSARYVVFLLPFYYLLVSRGGLA
                     VYGVARRNRVLAGAAGVAGLAVLGLFLAGSYRYYQPGGESATRYKPDYRAAAAYLAQR
                     VSPADVVVFVDDPPHGVDVSRFYWQGRMAAQVYDGRDPRLAAYQPRGAVYWVIGFFYY
                     DQDHQAALAAPAQGWAAVARFPGLVVARDPPGSATMIDRLDRLVGKLEALDRGAPAIG
                     TLRASVYQDRGQVPAALAIYRGPAGHVGQLGAEHLQTAQGFARRGLAERAWQQAMLSK
                     YNQAANPALHRWLAAALAATGATAASRTESDLAAGLAQRPDGR"
BASE COUNT          972 a         2114 c         2137 g          887 t
ORIGIN      
        1 gcggtgtagg ggccggtttc aaaccggccc gccgcccggc agaattagcg cggggcttcc
       61 ttttgcggtg taggggccgg tttcaaaccg gcctgccgcc caacgggatc gacgcgggca
      121 acggctaggg ccgccgccgg gtgaggcgcc tccgccagag gtgcggggcc accgagtcgc
      181 tgaggccgac cccggcagcc agcgccccgc cgagaaacac cgcgtggacc agcagcaaca
      241 gcccctgcaa gctctgattg gtcgccagtt cgagcatgcc gttgtagatg gtcacgccgg
      301 gcagcagcgg caggatgccc ggcaggatga agagcgacac gggcgcgtgc tggccgcggg
      361 cgaagcccca gctcaatacg cccaccacaa acgcggccag gaaggtggcg gccagcgggc
      421 cggcgttcac ctgcaacagg agcaggtagg ccaaccagcc cacggccccg gccacccccg
      481 ccgtgcccag ggcgaagcgc ggcacgtggt tcaagacggc gtagcaggcc gacgcggcaa
      541 aggcggcgac cacctggatc ggcatctgcc agacctgccc cgtgctgctc tgcaccgaga
      601 tctgttgccc cagattgagt gcgcccgcca gccccacgcc gacgccgcat gccagcgccg
      661 cgcccttcag caacgtttcc aacccgcgcg ccgccgcgct gagcaggttg ccggagatgg
      721 cgtcctgtac gctgatcagc agcgcggccc ccggcaccag cgtcatgatg ccgccggcga
      781 tgaccatgtc ggcatggatc ggcacgccga gcgacaccag cgccagcgcc accgccgtcg
      841 ccagggccgc gcccagggca tcgctgaaca gcgccgggat cttgctgcgt tgcagcagca
      901 cgccgatgag cagcaccggc acggtgctga cgaacgcggg caccacgtcg agcgggccgc
      961 cgcccagcag caaggcgacg cccgccgccg aggcggcccc gccggccagc cagaccggca
     1021 gtgtgtacgg atcgggcgcg cgggcgatgg cgtccaactc gcgcgccgcc tcggccacgc
     1081 tgacccggcc ctgcgccacc cgccgcgaca ggtcgttcac cgccgagacg cgggcatagt
     1141 gcagcgtgcg gctgggcacg cgatggacca gcgtgaccgg gtgggccatc tgcgggtcgt
     1201 cgagcgacac gtagatgccg gtggtcatca ccaggcactg gctgtaggcg atgccgaagg
     1261 cccgcgccag gatattgacc gtgtcttcga cgcgcgccgt ctccgccccg ctgctgagca
     1321 tgatggcccc cgcctgcacg gccaccgcca gcacctgctg cacatgcgag cgccccgccg
     1381 ccgggtgcga ttcgactgcc gtcaccgctg tcttctctct cccttcgtag ccctaagccg
     1441 ggccggtcgg tccctgttcc cggcagtata gcatacgccg gacccggcat ccgggtgcgg
     1501 gaccaaccta acccccctgg cccccttgtc cgccgcgtcg agcgccggcc aggcgacggg
     1561 gcgcgcacac gccgccaccc aggcgtgccc agcgtggttt tgccgaagcc ggcgggggcc
     1621 gcgatgagcg ccagcttgcg gcgctgcccc gtgttaaggc gtgcgatcag gtgcggccag
     1681 ggcacgactt gcggcggcgg tgggaatata gagtttggtg gccggcacgg ggatagatat
     1741 gaatccatta tagaagatct acccaaaccg cgggcagcgc tgccagagtg gacgccgggc
     1801 cgcaccgggt cccatgccgc caccgcgcgg aggattattg cacggcggcg agcgccgcgt
     1861 caatctcctg gcaggcctgg tcgagcgcct gccggctggt ctgcatcccg gcaaagacgc
     1921 gctcggtggc ctcgttgagg gcttgctgga tctgcggatt ggccgggccg ccccagacgc
     1981 ccggctggcc gtaactcgct ccctcgtaga gcagcctgga gatccggctg agcgtgctgg
     2041 acccctggaa gaaggggtgg gtctccatgc ccttcagcga gggcagggca aagccggtct
     2101 gcaggatcgc cccctcgttc tcccggctgg tgaggaacag cacaagcgcc gcggcggccc
     2161 tggggaacct ggtctgcgcc gcggcgcccc agcccgcgaa gcccaatgtc gtggccctgg
     2221 ccccgccctg gcccagcggc agcggcaccg cgtcatagtg gaccccgaag gccgctttgg
     2281 gatccgtcag gtaggggttg atccagccgc ccacgacaat gctggccgcg cgctgcttgg
     2341 cgaaggcttc cccctcccag ccggcgctga cgtcggccga gagcgcgagg acgtgatcct
     2401 ggtacaggcc atagaaccag tccagggtgg tgacccccgc gggactgttg atggcgcacc
     2461 ggttgttgtc gaggaccctg gcgccgttgg cgagggcgaa gttgccccag tagaggaaga
     2521 atacgagatc gctggcgaag ccgaacaccc gcgtctggcc gctgcccgtt gtgttcgctt
     2581 tcacccaggc cgtgaagtcg gcctgggtcc agccgggttt gggcggggtc gtgaccaggt
     2641 cggtgttgta gaagagggcc atgcttgtga agtccttggg caggccgtag acctgcccgt
     2701 ccgggccgag gaagacgccg gtgagcgcgg ggaagtagtc gtccttgctg cgcccgacct
     2761 cggtcagggc gggcctgagg tccaggagca cctgatgggg aatgagctgg tagggcagat
     2821 ccacgtcgag gtagaagacg tcgggttgcg cgccggcctt gacctgggcg aggagcttgg
     2881 tgccgtagtc ggacggctgc ggttcatagg tgatggtgat gccgggatag acctgctgga
     2941 agcgggcgag cgcgtcgcgg accacctgtt gctcggaggt atcgccccaa ctggagacgc
     3001 gcaagtgggc accaggttcg accgcgttct tccgataggc ggccgggtca aacggaacgg
     3061 cgccgggggc tgtcgtagcg gccggcgcca cggcgggggg cgcgaagccg gcgccgggtg
     3121 cgggaagggt gttcccgctg caggcgctca agcacagcgc gcccagcagc agcgctgccg
     3181 gcacgcgccc gcgggtccgc aaagccatgg cgaatcgcct ccttgctgca tccgggccat
     3241 gccgcgtggg aacgcgcgac gggccgcggg cagcgcgtgt tttcacttgt cgccgcgccc
     3301 ttctacgcaa cgatgcctgc ctgcgggtgc cgtctgtcca ggctatcgca cgcgcgcgga
     3361 tagttctcgt tgcctgggcc aggatacgca ttatgcgccc cggaaggcgg gaacgaggac
     3421 cggttcgcca tcctgcactg ccgactcatg ggcacagatg ccgggcgccg tccaatccgc
     3481 cgcggtatag ctgtcgatcc ggggcgcacg gccctccacg atgctccgga caaactcgtg
     3541 gacgagatgc gggtgcgagc cgccgtgatg gccgcgggtg aagccggcga tggccggcgg
     3601 cagcaggtgg ccccaatcgg gcacctcaat ctgcgcggtc gtcacggggc ggccgcgttg
     3661 tccggcgacg accggctcca gcgtgaagag taacggcgct tcgttttccc gcaaccactc
     3721 gaagccgcgc ttgtcgccat aaacgctgaa cgtctcggta tagggatggg ccacctggaa
     3781 ccaggagcgc gtcacctcgg cggccaggtt cgtgttcgct agacggaaca ggccggtctg
     3841 caaggggaac atgttgccgc ccggctgctg gatgtcgggc cgcagccggc cggagcccag
     3901 gcagatcact ttttcgacgc gcgtgcgggt cagcgccagc agcggcgcga gcgcgtgcgt
     3961 caggtagtgc atcggcggtt gggcgcgcca gtagcgggga aaatcgccct ccagatcctg
     4021 atagtacgtg ccgcggagga aggtgagcgt acccaggtcg ccgcgcctgt acagatcttc
     4081 gacgtagagg aactcgcgcg tatacgcccc ggtctccatc agcatgtagg ttttgccgga
     4141 cgcctgttgg gcggcgatga cccgctggat atcctccagg ctggtcgcca tgggcacggc
     4201 acaggcgcag tgcttgccgg cccccagcac ggccagggtt tgctcgacat ggagcggcac
     4261 cggcgtgagc agatgcacgg cgtcatagcg cgcatcggcg agaatctcgt ccaggccggc
     4321 aaaccgatcc gccacaccga actgatcgcc gatccgcgcc gtcagttcgg cgtccaggtc
     4381 acagatcccc actcgctcca cgtcggggtg ctgctgatag atcggcacga actccgcgcc
     4441 gaaatgcagg cccactacgg caatgcgcat gcggttgctc ctcgctcagt gccgcccacc
     4501 gccgggcggc caatccttga acagccaggg tggtgtcctg ccggccggtg cgaccccgcc
     4561 gcgttcgctt gggtgcggga cctgcgccgt caactgctca gcctgttggg ccaggatttg
     4621 ccggcatcct gcgtccaacg acagaatagc cgcccagtag gcatccgtca agcacgccag
     4681 cggctgaatc acagccctct gtgcccggcg gcgggtttcc gcccatggtt ggatccagca
     4741 gcgggcgggc cactgtcgct taacggacgg acgatagcgc gttggtccgc gccgcggagc
     4801 gccttgccgc ccgaataggg cggcaatgtg cagagccgag tttagagttg gaacatgcgt
     4861 gggactctgc cctagtgcgg gttccagcaa cctcttgggt gtagcggcgc gatttatcgc
     4921 gcccgtactc gtttatcttt agcggatgtg tccccagcag gtcgctggaa cccgcactag
     4981 cgcccgtcgg gccgctgagc caacccagcc gccagatcgc tttcggtgcg actcgcggcg
     5041 gtggcgcccg tggcggcgag cgctgccgcc agccagcgat gcagggcggg gttggcggcc
     5101 tggttgtatt tcgacaacat ggcctgttgc cacgcccgct ccgcgagccc gcggcgggcg
     5161 aacccctgcg cggtctgcaa gtgctccgcg cccaactgcc cgacgtgccc cgccggcccg
     5221 cgatagatcg ccagcgcggc gggcacctgc ccccgatcct ggtagaccga ggcgcgcagg
     5281 gtgccgatcg cgggggcacc ccggtcgagc gcctccagct tcccgacgag ccgatccagg
     5341 cgatcgatca tggtcgcgct gccgggcggg tcgcgggcga caacaagacc cggaaagcgg
     5401 gcgacggcgg cccagccctg cgccggcgcc gccagcgccg cctgatgatc ctgatcgtaa
     5461 tagaagaacc cgatgaccca gtaaaccgcg ccgcgcggct ggtaggcggc cagacgcggg
     5521 tcgcgcccat cgtagacctg cgccgccatc cggccctgcc agtagaaccg gctgacatcc
     5581 acgccgtggg ggggatcgtc gacaaacacc acaacgtcgg cggggctgac gcgctgcgcc
     5641 agatatgccg ccgcggcccg gtagtcgggt ttgtagcggg tcgcgctttc cccgcctggc
     5701 tggtagtagc gatagctgcc cgccaggaac agcccgagca cggccagccc ggccacgccc
     5761 gccgccccgg ccaggacgcg gttccgccgg gccaccccat acacagccag gccgcctcta
     5821 ctcaccaaca ggtagtagaa cggcagcagg aacacaacat agcgcgcgct cagtgtattg
     5881 gtcgtgctga gcacggcgag ttccgccggc gccagcaaga gcagggatcc gcaaatcagc
     5941 gcgccgtgcc gttcggcccg cgcgccgcgc actcctgccg cgaagcccag gcccgccagc
     6001 agcaggagcg ccccttgcag cggctcgccg agatgaaacg ggacgttggc ttgcgccagc
     6061 caatcgcgca gcatcaggcc gacggcgccg cgcatcgccc ggatctgggt
//
LOCUS       DASUYC010000292         2028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_862907,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000292 DASUYC010000000
VERSION     DASUYC010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862907"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VF276_04750"
     CDS             <1..445
                     /locus_tag="VF276_04750"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HEX6606206.1"
                     /translation="RDLDPRGQVRGRERAAALGLTNVRYELGDATDARSLATVDPPPQ
                     VVVASGVYELFNDTDLIRRSMAGIRAILPPGGRFLFTTQVRHPQLEFIANVLVNREGR
                     PWVMGCRSLAEVEGLARAAGFEVVRSALEPVGLFGVTICRVPGAA"
     gene            complement(593..>2028)
                     /locus_tag="VF276_04755"
     CDS             complement(593..>2028)
                     /locus_tag="VF276_04755"
                     /inference="COORDINATES: protein motif:HMM:NF024640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606207.1"
                     /translation="YDTGWVAAVSTASGAPRFPQSLHWLRRHQHGDGSWGAGSTEDQA
                     HPYYHDRLLSTLRAVLTLDAWSESPCDRRQVERGLAYIRAHAGDLAKDPVETVGFELI
                     FPTLLDEAAQRALDVPQEPFVPVRLTGAAKRARAPRHLAYDRRTPLAVSLEALGPHFE
                     VELASAVQEVNGSVGTSPSATAYLLQHCPDNETAVAFLDQVLQNDQAAGGMPAFFPIE
                     TFEYSWALYQLGQAQPAIYRHLAGSITPLLDYLYTTRTRAGWTSCAQATVKESDTTAV
                     CFSVLSQAGYTLNPALLYQYEEADHFRVYPFERNPSPSANAHILDALHYCDPRERAPR
                     VDKLIRFLTETRQPGGFWFDKWHVSPYYTTSHVVLAAQDWAPNLVAPAIDWLLAGQRP
                     NGSWGYYGATLEETAYAVLALLAWQRSGHCIPCDVIARAVDYLHAHYTRDDGAYPPLW
                     IAKTLYAPVLIAQAAVLAALLAAECPD"
BASE COUNT          333 a          640 c          731 g          324 t
ORIGIN      
        1 ccgcgacctg gacccgcgcg gccaggtgcg gggccgcgaa cgggcggcag cgctgggcct
       61 gaccaacgtg cgctacgaac tgggcgatgc gaccgacgcg cgctcgctgg cgacggtcga
      121 tccgccgccg caggtggtgg tggcttcggg cgtgtatgag ctgttcaacg acacggatct
      181 catccggcgc tcgatggccg gcatccgggc gatcttgccg ccgggcgggc gcttcctgtt
      241 cacgacgcag gtgcgccacc cgcagttgga gttcatcgcc aacgtgctgg tcaaccgcga
      301 gggccggccc tgggtgatgg gctgccggtc gctggccgag gtggagggcc tggcccgcgc
      361 cgcgggcttc gaggtggtgc gctcggcgct ggagccggtc gggctgttcg gcgtgacgat
      421 ctgccgggtg ccgggcgcgg cctgagccgc ccatcaccca agcattgtca cgctgagcgc
      481 agcgaagagt ctcgtcttgt atcccaacga gacgagattc ttcgctgcgc tcagaatgac
      541 aattctgccg tgacacacga ctcagaagga ccgggatgac ggaacgcggg gctcagtccg
      601 ggcattcggc ggccagcagg gcggccagca cggcagcctg cgcgatcagc accggggcgt
      661 agagcgtctt ggcgatccag agcggcggat aggcgccatc gtcgcgggtg tagtgcgcat
      721 ggagatagtc taccgcgcgg gcgatcacat cgcatgggat gcaatgaccg gagcgctgcc
      781 aggccagcag ggccagcacg gcgtaagccg tctcttcgag cgtggcccca tagtagcccc
      841 aactgccgtt ggggcgctgc ccggccagca accagtcgat ggccggcgcg accagattgg
      901 gcgcccagtc ctgggcggcc agcaccacat ggctcgtcgt gtaatagggg ctgacgtgcc
      961 atttgtcaaa ccagaaccca cccggctggc gcgtctcggt caggaaccgg attagcttgt
     1021 ccacccgcgg cgcgcgctcg cggggatcgc agtaatggag cgcgtcgagg atatgggcat
     1081 tggcgctcgg cgacgggttg cgctcgaagg ggtagacccg gaagtgatcg gcttcctcgt
     1141 actggtagag cagcgccgga ttgagcgtgt agccggcctg actcaagacc gagaagcaga
     1201 ccgccgtcgt gtcggactct ttgaccgtgg cctgggcgca cgaggtccac ccggcgcggg
     1261 tgcgggtggt atacaggtag tctaacagcg gggtaatgct gccggccagg tgccggtaga
     1321 tagcgggttg cgcctgcccc aactggtata gcgcccacga gtattcgaag gtctcgatgg
     1381 ggaagaaggc aggcatgccc ccggcggctt gatcgttctg cagcacctga tcgagaaagg
     1441 ccaccgccgt ctcgttatcg ggacagtgct gcaacagata ggcggtggcc gacggtgagg
     1501 taccaacact accgttcacc tcttgcacgg cactggcgag ttccacctca aagtgcggcc
     1561 cgagcgcttc caggctgacg gccaggggcg tgcggcggtc atacgccaga tgccggggcg
     1621 cgcgggcgcg tttggcggcc cctgtcagcc gcacgggcac gaacggctcc tgcggtacgt
     1681 ccagggcccg ctgcgcggct tcatcaagga gcgtggggaa gatcaactca aaacccaccg
     1741 tctccaccgg gtccttggcg agatcgccgg cgtgggcgcg gatataggcc aggccgcgct
     1801 ctacctgccg acggtcgcac ggcgattcac tccaggcgtc gagggtgagc acagcgcgca
     1861 gcgtgctgag caagcgatcg tggtaataag gatgggcctg atcttcggta ctgccggcgc
     1921 cccaactgcc gtcgccgtgc tggtgccggc gtaaccagtg caggctctgg gggaagcgcg
     1981 gtgccccgct cgccgtactc accgcggcca cccagccggt gtcgtaag
//
LOCUS       DASUYC010000293         3701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1811772, whole genome shotgun sequence.
ACCESSION   DASUYC010000293 DASUYC010000000
VERSION     DASUYC010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1811772"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1388
                     /locus_tag="VF276_04760"
     CDS             <1..1388
                     /locus_tag="VF276_04760"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEX6606208.1"
                     /translation="PAEFGLVSMALLVITALDILRDFGIGASLIYRQRDGQAAANLAF
                     VLSAVIGVILFVGNWLLAPFVTVFFKTNGPEETATVTGLVQVLGFSLLFASLGSTQDA
                     LLQKAIDYRRRMIPEVGRTLIKGVLQVALALAGFGVWSLVWGQVIGEACATILLWTVS
                     SWRPTRLLDRTLLRPMVNYGTQIMMVGGLGWLVADIDYLIIGRFLGDAALGLYTLAFR
                     IPELIIRNLAQAVSNVAFPVAARFQEDKAAMRHAYLTMQHYMLAILAPLGFGLFAVTP
                     SLIHILFQDKWEPAIPVMELLSIYMVLSGISHWPGVVYKAVGRPDILNRLSFLKLVLL
                     APTLWWAATTYGIVGVGWSQVAVRIVVILIDMWTVSRFVQISMRANLQAIWPPLAASA
                     IMAAAVRLVFLLDPRESSIPLLIVAIITGGAVYAGALWLLDRPTVSALLDLARGMVAR
                     RRRIAPAPDAG"
     gene            complement(1385..2038)
                     /locus_tag="VF276_04765"
     CDS             complement(1385..2038)
                     /locus_tag="VF276_04765"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEX6606209.1"
                     /translation="MLVVNRERTPLRPPRPRRRAIGRLLALALLLPILWVVADLRVVL
                     DGAEADHAAPADVIIVLGCQAWTSAGISPCIRARADHAADLYRRGLARHIIPSGWASA
                     HGPSEASALMLVLLAGGVPRSAIVADDQAQNTIQNIRNSQAIMAAHGWHTAILVTEPY
                     HINRAALIARDAGLAAVASPALASPDWRDPWSRVWHVERDTLSLMLYQVKQVLGIHY"
     gene            2037..2615
                     /locus_tag="VF276_04770"
     CDS             2037..2615
                     /locus_tag="VF276_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606210.1"
                     /translation="MPLSHIEPASRPDAPALPPWATALPLWAAGGWIWISAPPAAPLR
                     YWPGGPAGALAPAPLPHLEGLPPGPAYPVIVLDYQAGPVPPAGWWALLAAALAPGGVL
                     FVCGPHPAGLAPIIHAAAPDLQPADPHLIEPGQDGYWFLRPARPAFRRLIARQLQPPL
                     SVRAWLTQVRRRVRPPAERVPCLPVWRAGGSP"
     gene            2612..3646
                     /locus_tag="VF276_04775"
     CDS             2612..3646
                     /locus_tag="VF276_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606211.1"
                     /translation="MKALLGGILTPDLLTAVAGHPVRPPLGLVQGGPTGRSRDKRILF
                     LIEPGARHPFALVKWAQGAEAAGLARETAALAQIHAAGDPVLDASCPPSWGPFAIAPG
                     VQVSVERYLPARGAYAQLRTSLWPRPLVADHFRRAAGWLDHFARATHGPTRPFDQALL
                     DEAIAAPLETCAARFDLPAAHVRATLDAARAHLGRPVALTAEHGDFWLANLLLPPAPD
                     AAYVVDWEYFAPAALPSFDMLLFCMTYALEYPWRPFGWIDPQTAVARAFVQPELLRPH
                     ISALLARGCEAAGLPPPLLPALLPAMLARMALRHAGTTNSTWLAALAAWWARPAAGWL
                     ETWAADAGAP"
BASE COUNT          461 a         1363 c         1281 g          596 t
ORIGIN      
        1 cgcccgccga gtttggtctg gtcagcatgg ccttgctggt catcacggcg ctcgacatcc
       61 tgcgcgattt cggcatcggc gcctcgctca tctatcgtca gcgcgacggc caggccgccg
      121 ccaatctcgc cttcgtgctc agcgccgtca tcggcgtgat cctgttcgtg ggcaactggc
      181 tgctggcccc gttcgtcacc gtcttcttca agaccaacgg cccggaggaa acggccaccg
      241 tcaccggtct cgtgcaggtg ctgggcttta gcctgttgtt cgccagcctg ggcagcacgc
      301 aggatgcgct gctgcaaaag gccatcgact atcgccggcg catgatcccc gaggtggggc
      361 gcacgctgat caagggcgtg ttgcaggtgg cgctggcgct ggccggcttc ggggtctgga
      421 gcctcgtctg gggccaggtg atcggcgaag cctgcgcgac gatcctgctc tggaccgtct
      481 cgtcgtggcg gcccacgcgg ctgctcgatc gcacgctgct gcggcccatg gtcaactacg
      541 gcacgcagat catgatggtg ggcggactcg gctggctggt cgccgacatc gactacctga
      601 tcatcgggcg cttcctcggc gacgcggccc tgggtctcta caccctggcc ttccgcatcc
      661 cggagctgat catccgcaac ctggcccagg cggtgtcgaa cgtggccttc ccggtggcgg
      721 cgcgcttcca ggaagacaag gcggccatgc gccatgccta cctgaccatg caacactaca
      781 tgctggcgat cctggccccg ctgggcttcg gcctcttcgc cgtcaccccg tcgctgatcc
      841 atatcctctt ccaggacaag tgggagccgg ccatcccggt gatggagttg ctctcgatct
      901 acatggtgct aagcgggatc agccactggc cgggcgtggt ctacaaagcc gtcgggcggc
      961 ccgacatctt gaaccggctc agcttcctga aactggtgct gctggcgccc acgctctggt
     1021 gggcggccac cacctacggc atcgtgggcg tgggctggag ccaggtggcc gtgcgcatcg
     1081 tggtgatcct gatcgatatg tggacggtgt cacgcttcgt gcagatcagc atgcgggcca
     1141 acctgcaggc catctggccg ccgctagccg ccagcgcgat catggccgcg gcggtgcgcc
     1201 tggtgttcct gctcgacccg cgcgaatcca gcatcccgct gctcatcgtc gccatcatca
     1261 ccggcggcgc ggtttatgcg ggcgcgctct ggttgctgga tcgcccgacg gtcagcgcct
     1321 tgctcgatct ggcgcgcggc atggtggccc ggcgccggcg catcgcaccc gcacccgatg
     1381 ccggctagta gtggataccc aggacttgct taacctggta cagcatcagc gataacgtat
     1441 cccgctcgac gtgccagact cgcgaccagg ggtcgcgcca gtcggggctg gcgagcgcgg
     1501 gcgaggcgac agcggcgagt cccgcgtcgc gggcgatcag cgccgcccgg ttgatgtggt
     1561 acggttccgt gaccaggatg gccgtgtgcc agccgtgagc ggccatgatg gcctggctgt
     1621 tgcggatatt ctggatggtg ttctgggcct ggtcatcggc cacgatggcg ctgcgcggga
     1681 cgcccccggc cagcagcacc agcatcaggg cgctggcctc gctggggcca tgcgccgacg
     1741 cccagccgct ggggatgatg tgccgggcca ggccgcgccg gtagagatcg gcggcgtgat
     1801 cggcgcgggc ccggatacac gggctgatcc cggcggatgt ccaggcctgg caccccagca
     1861 cgatgatcac atccgccggg gcggcatggt cggcttccgc gccgtccagc acgacgcgca
     1921 gatccgccac cacccagagg atcggcagca acaacgccag ggccagcagc cgcccgatcg
     1981 cgcggcgacg ggggcgcggc gggcgcaaag gagttcgttc gcgattgacg accaacatgc
     2041 cactctcaca tatcgagccg gcgagccgac cggacgcgcc ggcgctgccg ccgtgggcga
     2101 cggccctgcc cctctgggcg gccggcgggt ggatctggat tagcgcgccg ccggccgccc
     2161 cactacgcta ctggccgggt ggcccggccg gcgccctggc cccggcgccg ctaccgcacc
     2221 tggagggctt gccgcccggc ccggcctatc ccgtgatcgt gctggactac caggcggggc
     2281 cggtgccgcc tgccgggtgg tgggcgctgc tcgcggcggc cctggcgccc ggcggcgtgc
     2341 tgtttgtctg cggcccgcac cctgccggcc tcgcccccat tatccatgct gccgcgcccg
     2401 atctgcaacc cgccgacccc cacctgatcg aacccggcca ggacggctac tggtttttgc
     2461 gcccggcccg cccggccttc cgccgcctga tcgcccggca actccagccg ccgctgagcg
     2521 tgcgcgcctg gctcacccag gtgcggcgcc gcgtgcgccc cccggccgag cgtgtgccct
     2581 gcctgccggt ctggcgggcg gggggtagcc cgtgaaggcc ctgctcggcg gcatcctgac
     2641 ccccgatctg ctgacagccg tggccgggca cccggtgcgc ccgccgctgg gcctcgtcca
     2701 gggggggccg accgggcgca gccgcgataa gcggatcctg ttcctgatcg agccgggcgc
     2761 ccggcacccg tttgccctgg tcaagtgggc gcagggtgcg gaagccgccg ggctggcgcg
     2821 cgagaccgcg gccctggccc agatccatgc cgcgggcgac ccggtgctcg acgcgagttg
     2881 cccgcccagt tgggggccgt tcgccatcgc gcccggcgtg caggtcagcg tcgaacgcta
     2941 cctgccggcg cgcggcgcct atgcccagct acgcaccagc ctctggccgc gcccgctcgt
     3001 ggcagatcat ttccgccgcg ccgccggctg gctcgaccac tttgcccgcg ccacacacgg
     3061 gcccacccgc cccttcgacc aggcgctgct cgacgaagcg attgccgcgc ccctggagac
     3121 ctgcgccgcg cgcttcgacc tcccggcggc gcacgtgcgc gccaccctgg acgcggcgcg
     3181 cgcccacctg ggccgccccg tcgccctcac cgccgagcat ggcgatttct ggctggcgaa
     3241 tctgctgctg ccgcccgcac cggacgccgc ctatgtggtg gactgggaat acttcgcccc
     3301 ggcggcgctg cccagcttcg acatgctgct cttctgcatg acctacgcac tggaatatcc
     3361 ctggcggccc ttcggctgga tcgatccgca aaccgcggtg gcccgcgcgt ttgtgcagcc
     3421 ggagttgctg cgcccgcata tcagcgccct gctggcgcgg ggttgcgagg ccgccgggct
     3481 gccgccgccg ctgctgcccg cgctgctgcc tgccatgctg gcgcgcatgg ccctgcgcca
     3541 cgcgggcacc acgaacagca cctggcttgc ggcgctggcg gcctggtggg cgcgcccggc
     3601 ggccggctgg ctggagacct gggcggcaga cgccggggcg ccgtaggggt tactggcgga
     3661 aggtgaactc cgccgccgcg cgcgggctga ccagcccttt a
//
LOCUS       DASUYC010000294         3639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_107504,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000294 DASUYC010000000
VERSION     DASUYC010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107504"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(393..2243)
                     /locus_tag="VF276_04780"
     CDS             complement(393..2243)
                     /locus_tag="VF276_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6606212.1"
                     /translation="MPGPGPIHEDELLGKAYDHRMMQRLLGYLRPYRKRMVGALVLII
                     TLGAVELAPPYLTKLAIDQYIVPGDLSGMPLVLGLFLASLIAAFGLRYSQNLIMQRMG
                     QDVMYDMRSNIFGHLQRQSLSYFDRNPVGRMMSRLTSDVDALNELLSSGVVAIVGDFV
                     ALVGIVIVMLLLDWKLALVSLAVLPVIYVVSQRFQRWMRRTYRDQRIRLARVNAFLQE
                     NLSGMLVLQLFNREKRQAEEFDRLNKHYLEANVEALRAFALFFPIINLCYALAVALLL
                     WYGAGAVLSQVVTVGVLVAFFQYTERAFQPIRDLAEKYNIMQAAMAAAERIFVMLDEP
                     ISIEDATAPVTLGQVRGDIEFRHVNFEYNPGEPVLKDVSFQIPAGTSVAIVGATGAGK
                     TSIISLLGRFYDVQDGAILLDGVDVRRVRQQQLRRHIGVVLQDAVLFSGTIASNIRLL
                     DDSITDAQVRHAAEFVNAAGFIEALPSGYEHEVKERGTNLSVGQRQLLAFARAIAFNP
                     EVLLVLDEATSSVDTETEALIQDALEKLMEGRTSIIIAHRLSTIRHVDRIIVLHKGRV
                     VEEGTHDELMARGNFYYRLYQLQYQEQEARERRVEAEKRRLHQQADAAHR"
     gene            complement(2258..>3639)
                     /locus_tag="VF276_04785"
     CDS             complement(2258..>3639)
                     /locus_tag="VF276_04785"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6606213.1"
                     /translation="DMSAVRGFIGPGILNTSNTVLMFIIAAVLMFGIDAKLAVIAVLF
                     LPLVSIAFTVIGNKMHQRYEKVQAQFGNLSTRAQENFSGIRVIKAYAQEDLEIARFAK
                     ANREYVRLAMDYQRLSGLLWPLMTFVLGVTAGIILFVGGSEVIAGHITLGQFVQFNGY
                     LGMLSWPMIALGWVVNLYQQGVASMGRISEVMRQEPAIADRPTALPVTAIKGEIEFRD
                     VGLSYGGQRVLNGLSFHVPAGQTLAILGATGAGKTSIVNLITRVYEAQQGQVLIDGVD
                     VRDLPLAVLRRSVGMVPQDTFLFSVALRENVSFGVDKVDEGRLAQAVTVARLSNDIDQ
                     FPQGLDTVIGERGVTLSGGQKQRTAIARAVMRDPAILILDDALSSIDTATQAEILQGL
                     QSVLAGRTSLIIAHRISTVKAADQIIVLDDGHVIEQGRHQELVARRGVYAQMYRRELL
                     SQELEVEVE"
BASE COUNT          647 a         1197 c         1151 g          644 t
ORIGIN      
        1 aggtctcgtc caattccaga tcttcgggga ggacggtccc gcccccggtg tagatagcgc
       61 ccggcatccc ggcgggcacg tggcgtgtcc cttccgtgac cgggtcctgc tccacttcat
      121 cgctgtccat atgtataacc ctcctgttcg gttgccttag tgcggtttcc agcgaccttt
      181 tatgtgtagc ggcgcgattt atcgcgcccg tactcgttgg tctttagcgt atgctatccc
      241 caacaggtta ctggaagctg cattaggccg cgcggcacgc ccggcagccc ataggtccga
      301 tgaaagcatg gatagtgcca ttcgccggac agcaaaacgc gcccggccaa actaaccggg
      361 cgcgtggcgg ttgccgtgag ttgccggcga ccctagcggt gcgcggcgtc ggcttgctgg
      421 tgcaggcggc gcttctcggc ctccacgcgc cgttcgcgcg cttcctgctc ctggtactgc
      481 agttggtaga gccggtagta gaagttgccg cgcgccatca actcgtcgtg cgtgccttcc
      541 tcgaccacgc ggcccttgtg cagcacgatg atgcgatcca cgtggcggat ggtggagagc
      601 cggtgggcga tgatgatgct ggtgcggcct tccatcagct tctccagcgc gtcctggatc
      661 agcgcctcgg tctccgtgtc aacgctggaa gtggcttcgt cgaggaccag cagcacttcg
      721 gggttgaagg cgatggcccg cgcgaaggcc agcaactggc gctggcccac cgagaggttg
      781 gtgccgcgct ccttcacctc gtgctcatag ccggagggca gggcctcgat gaagccggcg
      841 gcgttgacga actccgcggc atggcgcacc tgggcgtcgg tgatcgagtc gtccagtagc
      901 cggatgttgc ttgcgatggt tccggagaag agcacggcgt cctgcaacac gacgccgatg
      961 tggcggcgca actgctgctg gcgcacccgg cgcacgtcca cgccgtcgag caggatcgcc
     1021 ccgtcctgca cgtcatagaa gcggcccagc aggctgatga tgctggtctt gcccgcgccg
     1081 gtggcgccca cgatggcaac gctggtgccc gccgggatct ggaagctgac atctttaaga
     1141 accggctcgc cggggttata ctcgaagttc acgtggcgga actcgatatc gccgcgcacc
     1201 tgccccagcg tcaccggggc cgtggcgtcc tcgatgctga tcggctcgtc gagcatcacg
     1261 aagatgcgct cggcggcagc catggcggcc tgcatgatgt tgtacttctc ggccaggtcg
     1321 cggatgggct ggaacgcccg ctcggtgtac tggaaaaacg ccaccagcac gcccaccgtc
     1381 accacctgcg acagcaccgc gccggcccca taccagagca gcaacgccac ggccagcgcg
     1441 tagcagaggt tgatgatcgg gaagaagagc gcgaacgcgc gcagggcctc gacgttcgct
     1501 tccagatagt gcttgttgag ccggtcgaac tcctcggcct ggcgcttctc gcggttgaac
     1561 aactgcagca ccagcatccc cgacaggttt tcctgcaaga aggcgttcac gcgggccagg
     1621 cgaatccgct ggtcgcgata ggtgcgccgc atccagcgct ggaaccgctg gctcaccacg
     1681 tagatcaccg gcagcaccgc caggctgacc agggccagct tccagtcgag cagcagcatg
     1741 acgatgacga tgcccaccag cgcaacgaaa tcgcccacga tggcgaccac acccgaactg
     1801 agcagttcat tcagcgcgtc cacatcgctg gtcagccggc tcatcatgcg gcccaccggg
     1861 ttgcgatcga aataggagag cgactgccgc tgcaaatggc cgaagatgtt ggaccgcatg
     1921 tcgtacatga catcctggcc catgcgctgc atgatcaggt tctgcgaata gcgcagcccg
     1981 aacgccgcga tcaggctggc gagaaacagc cccagcacca gcggcatccc cgacaggtcg
     2041 cccggcacga tatattggtc gattgccagc ttggtcaggt acggcggcgc cagttcgacc
     2101 gcgccgaggg tgataatcaa cactaaggcc ccgaccatcc gtttgcggta gggccgcaga
     2161 tagcccagca gccgctgcat catgcgatga tcataggctt tgcccagtaa ttcgtcttcg
     2221 tgtataggac cgggtcctgg cataaacgcg tacctcgcta ctcgacttcc acttccaatt
     2281 cctggctcag cagttcgcgg cgatacatct gcgcgtacac gccgcggcgg gccaccagct
     2341 cctggtgccg gccctgctcg atgacatgcc catcgtcgag gacgatgatt tgatcggccg
     2401 ctttcaccgt cgaaatccga tgggcgataa tcaggctggt gcgtccggcg aggacgcttt
     2461 gcaggccctg gagaatctcg gcctgcgtgg ccgtgtcgat gctcgacagg gcatcgtcca
     2521 ggatgaggat ggccgggtcg cgcatcacgg cgcgggcgat ggccgtgcgc tgcttctggc
     2581 cgcccgacag ggtgacgccg cgctcgccga tcaccgtgtc cagcccctgc gggaactggt
     2641 cgatatcgtt gctcaggcgg gccacggtga cggcctgggc cagccggccc tcgtccacct
     2701 tgtccacccc gaagctgacg ttctcgcgca gggccaccga gaacaggaac gtatcctgcg
     2761 gcaccatgcc gactgagcgc cgcaacacgg ccagcggcag gtcgcgcacg tcgacgccgt
     2821 cgatcagcac ctgcccctgc tgcgcctcat agacccgcgt gatcaggttc acgatcgagg
     2881 ttttgccggc tcccgtggcg cccaggatcg ccagggtctg cccggcgggc acatggaagc
     2941 tgagaccgtt gagtacccgc tggccgccat agctcaagcc cacgtcgcgg aactcgatct
     3001 cgcccttgat cgccgtgacc ggcagcgccg tgggccggtc ggcgatggcc ggttcctggc
     3061 gcatcacctc ggagatgcgg cccatcgagg ccacgccctg ctggtagagg ttgaccaccc
     3121 agcccagggc gatcatcggc caactgagca tgccgagata gccgttgaac tgcacaaact
     3181 ggccgagcgt gatatgcccg gcaatgacct cgctgccgcc gacgaacagg atgatacccg
     3241 ccgtgacgcc cagcacgaag gtcatcaggg gccagagcaa cccggagagc cgctggtaat
     3301 ccatcgccag ccgcacatac tcccggttgg ccttcgcgaa gcgggcgatc tccaggtctt
     3361 cctgcgcgta ggccttgatc acgcggatgc cggagaaatt ctcctgcgcc cgcgtggaca
     3421 ggttgccgaa ctgggcctgc accttctcat agcgctggtg catcttgttg ccgatgacgg
     3481 taaaggcgat gctgaccagc ggcaggaaca gcacggcgat cacggccagc ttggcgtcga
     3541 tgccgaacat cagcaccgcg gcgatgataa acatcaacac cgtgttggac gtgttcagga
     3601 tgccggggcc gataaagccg cgcacggcgc tcatgtcgt
//
LOCUS       DASUYC010000295         2313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1448527, whole genome shotgun sequence.
ACCESSION   DASUYC010000295 DASUYC010000000
VERSION     DASUYC010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1448527"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2313
                     /locus_tag="VF276_04790"
     CDS             <1..>2313
                     /locus_tag="VF276_04790"
                     /inference="COORDINATES: protein motif:HMM:NF025930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606214.1"
                     /translation="AVRYPTEDAAPTHAVLRAAGRPAPPTEAGAPALPHHIEPPDRLA
                     ERYTAHPDALRLTAAVAESCRAELADLRAQGDANSAQAALETTVDEALAAAGRERDAY
                     IAAEVAAVRRLGLADALLAARRVAATARGQGLVLGAPGGLIAEGRLPALLGLAPADPF
                     GAPARWLAGDARPGAWTPALAVPAGRRPSLVATLQAREGGARATPAPDLLPAGGRRPL
                     GVAGALAAAGRVFDLAPATRAALETAWVQTTPAARARRWQDAAPALAPETAENLLRIV
                     TALAEAPGPPLADAEALLVVPSGACLPVVALPGAPGGRAAAWTAADCEALGASVVRLW
                     GDPDLDMLQRGLDLAGEPLPTVPTDAMRLLLSSGDIAGLPLPVVAEDSADGLETTPDV
                     LAAFCAALLPPEPEDVPGWAALGALLYGPHGPHPWLRTYLRRRVGGAPVTYRFPNLAP
                     ALDLTWGLPLYCEQLAILHLLQRQDGAVPPQALMQVQEWLGQAEMWATDYSAAAAAAE
                     RLHLALRLKVTHPAAFLAAALESAVLAGDTAAETTLTLEARRHGIELRPPAIQTSAAG
                     FTLEAPAPRAVPPAEPGNARRAPAPPPLPAIRWGLATGRGTLPAAAALVAARAAQPDG
                     RFASPAALCLAAVAADVPPGFLAVLIRAGACDSLGARPALLAALPAAVAEARQTHAAQ
                     QVATQGGQAGLFPVEPPVEAAPGTEAPADGPATDASTETPVESTTETAAPIEVDKAGW
                     RAWEATLIGHTFTVLPSAEPVLGAGAVVRG"
BASE COUNT          242 a          910 c          884 g          277 t
ORIGIN      
        1 cgccgtccgc tatcccaccg aggatgccgc gcccacgcat gctgtgctgc gcgccgccgg
       61 gcgacccgcg ccccccaccg aggccggcgc cccggccctg ccgcaccaca tcgagccgcc
      121 cgaccggctg gccgagcgct acacagccca tcctgacgcc ttgcggctaa ccgccgccgt
      181 cgccgagtcg tgccgcgccg aactggccga tctgcgcgcg cagggcgacg cgaactccgc
      241 gcaagccgcg ctggagacca ccgtggacga ggccctggcc gccgcgggac gcgaacggga
      301 tgcgtacatc gcggcggaag tggccgccgt gcgccgcctg ggcctggccg atgcgctgct
      361 ggcggcccgc cgtgtggcgg caacggcgcg ggggcagggg ctggtgctgg gcgcgcccgg
      421 cggcctgatc gccgagggcc ggctgccggc gctgctggga ctcgcgcccg ccgacccgtt
      481 tggcgccccg gcccgctggc tggccggcga cgcgcggccc ggcgcctgga ctcccgcgct
      541 ggctgtgccc gccgggcggc gccccagcct ggtggcgacc ctgcaagccc gcgaaggcgg
      601 tgcccgcgcg accccggcgc ccgatctgtt gcccgccggg gggcggcgcc cgctgggggt
      661 ggccggggcg ctggccgccg ccgggcgcgt gttcgacctg gccccggcca cgcgggcggc
      721 gctcgaaacc gcctgggtgc agaccacgcc cgcggcccgt gcccgccgct ggcaggacgc
      781 ggcgcccgcg ttggccccgg aaaccgccga gaacctgtta cgcatcgtca cggcgctggc
      841 cgaggcgccc ggtcccccgc tggccgacgc cgaagcactg ttggtggtgc cgtcgggtgc
      901 ctgcctgccc gtggtcgcgc tgcccggcgc accgggcggg cgcgccgccg cctggaccgc
      961 ggcagattgc gaggcgctgg gcgcgagcgt cgtgcggctg tggggcgacc ccgacctcga
     1021 catgctccag cgcgggttgg acctggccgg ggaaccgctg cccaccgtgc ccaccgacgc
     1081 catgcgcctg ctgctctcgt ccggcgatat tgccgggctg ccactgcccg tggtggcgga
     1141 ggacagcgcc gacggactgg agacgacgcc cgatgtgctg gccgctttct gcgcggcgct
     1201 tttgccgccc gaaccggagg acgtgcctgg ctgggcggcg ctgggtgcgt tgctgtacgg
     1261 cccgcatggg ccgcatccct ggttgcgcac ctacctgcgg cggcgcgtcg gcggcgcccc
     1321 ggtcacgtac cgcttcccga atctggcgcc cgcgcttgac ctgacctggg gcctgccgct
     1381 gtactgcgag cagttggcga tcctgcacct gttgcagcgc caggacggtg ccgtcccgcc
     1441 gcaggcgctg atgcaggtgc aggagtggtt ggggcaagcc gagatgtggg ccacggacta
     1501 cagcgccgcc gcagccgccg ctgagcgcct gcacctggcg ctgcggctca aggtgacaca
     1561 ccctgcggcg ttcctggctg ccgcgctgga gagcgcggtc ctggccgggg acacggcggc
     1621 ggaaaccacg ctgacgctgg aggcacgccg ccacggcatc gaactgcgcc cgcccgctat
     1681 tcagaccagc gccgccgggt tcaccctgga agcacccgcg ccccgcgccg tgccgcccgc
     1741 cgagccgggc aacgcccgcc gggcgcccgc gccgccgccc ctacccgcga tccgctgggg
     1801 gctggccacc gggcggggca cgctgcccgc agccgcggcg ctggtcgcgg cgcgtgccgc
     1861 ccagcccgat ggccggttcg cctcgccggc cgcgctctgc ctggccgcgg tggcggcgga
     1921 tgtgccgccc ggcttcctgg ccgtgctcat ccgcgccggg gcgtgcgata gcctgggcgc
     1981 ccgccccgcc ctgctggccg cgttgcccgc cgctgtggca gaggcccggc aaacccacgc
     2041 cgcgcagcag gtggcgacgc agggcgggca ggcgggcctg ttcccggtcg agccgccggt
     2101 ggaggccgcg ccgggcacag aggccccggc ggacggcccc gctaccgatg cctccacgga
     2161 aaccccggtc gagagcacga cggagactgc cgctccaatc gaggtggaca aggccgggtg
     2221 gcgcgcctgg gaggcaaccc tgatcgggca taccttcacg gtgctgccgt ccgccgagcc
     2281 ggtgttgggc gcgggcgccg tggtgcgcgg cag
//
LOCUS       DASUYC010000296         4015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_59192,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000296 DASUYC010000000
VERSION     DASUYC010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59192"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1384)
                     /locus_tag="VF276_04795"
     CDS             complement(<1..1384)
                     /locus_tag="VF276_04795"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6606215.1"
                     /translation="MSLKLRLSLFATVLMLLTLALISFVSYHYVADAFARQLDAALED
                     RSAEVIDNYLRLGPVANSFDLPPTRAQSVPVYVQIVNHLGEKVYPPGPELLPIDRTLI
                     HQALKGEMVTRDIGADPTTAMRVRYAPLRDPDNKTPVQLAVEVGTPLASTQSALALMR
                     LLLVSTTLFSSALIGFGVWFAASRTLAAVDRVTHTARTIELSQSLGARIPEDAATGDD
                     EMGRLVRTFNNMLDRISVAFDAQRQFVADSSHELRSPLTVIRGNLDLLRRTHDAEERH
                     TIERLIEDEAARMSRLVDNLLFLAQLGEMEQAGAPLDRRPVELDSLLLTVYQQARSMS
                     SRHTILLGDEDAITIDGDRDQLQQLLLNLVDNAIKYTPPGGTIILSLRAEGEDALLEV
                     KDTGIGIPPEDLPHIFDRFYRVDKARSRRIGGAGLGLSIVKGIATSHGGDVEVVSAPG
                     EGTTFRVRLPR"
     gene            complement(1389..2081)
                     /locus_tag="VF276_04800"
     CDS             complement(1389..2081)
                     /locus_tag="VF276_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6606216.1"
                     /translation="MMHILAVDDDPRILDLLRRGLTYEGYSVAAVEDGSAALAAARDQ
                     PPDLVVLDWMLPGMDGLEVCRRLRAGGDVPILLLTARSGVPDKVAGLDSGADDYLVKP
                     FSFDELLARVRALLRRRQAAATSPAVLRVGRLTVTPASREVVYDGRALEVSGKEFDVL
                     ELMLRHPRQVVTRGQFYDRIWGYDFGGESNILEVYIRYLRQKLEKVGAGSIIQTVRGV
                     GYMLRESEEDKG"
     gene            2334..3074
                     /locus_tag="VF276_04805"
     CDS             2334..3074
                     /locus_tag="VF276_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606217.1"
                     /translation="MPYPSYLNIDEYSAIRQNGCIPLAWLAALRDAQPLDTEAGEFWG
                     WESTPWETTDSIDRAINVLQRDEYLWAYFNILSLLLEEIQQVPTEEPISFDVSEFAAV
                     DPARAQAARAASDNFRAMLRMLHRGERDAALAALRQLSADLNLDAGLPFTGDLDADIA
                     AMGGGENALQELTWSIMGEIYEGPPERADWYTPEHYRINFWHWLAAPGPASAPEGAPA
                     EAPPADGTTGNGSVIAGVVNHNGSAPEL"
     gene            3079..>4015
                     /locus_tag="VF276_04810"
     CDS             3079..>4015
                     /locus_tag="VF276_04810"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX6606218.1"
                     /translation="MAINPFRGPRPPDAVPHPAGSLGPARPNHGRLAATLAYGLPAAA
                     LAAAGAGLYRRLHRSVPPIDGTLACPVTAPVRVVRDHWGIPHIYAENAADLFTAQGYV
                     QAQDRLWQLDFQRRVAAGRLSEIFGPPTLANDLLLRRFSLRQAAEAEAAALSEEATAA
                     LTAYCAGVNAYIAWAREHRALPAEFAVLAYEPEPWTPADGISWSKVMAWGLGGNWESE
                     LVRARLVNHLGPVRAAALEPAYPPGQPLTAEPGVAFSGLDDLFAQLLEQYEDLVQMTG
                     IGEMMQSAIPASNNWVVSAERSATGHALLANDPHLP"
BASE COUNT          669 a         1425 c         1305 g          616 t
ORIGIN      
        1 cgcggggcag gcgcacccgg aaggtggtgc cctcgcccgg cgcgctgacg acctcgacat
       61 cgccgccgtg gctcgtggcg atccccttga cgatggagag ccccaacccc gccccgccga
      121 tgcgccgcga acgggcctta tcgacccggt agaagcggtc gaagatatgc ggcaggtcct
      181 ccggcgggat gccgatgccc gtatccttca cttcgagcag ggcgtcctcg ccctcggcgc
      241 gcaggctcag gatgatcgtg ccgccgggcg gcgtgtactt gatcgcatta tcgaccaggt
      301 tcaacagcaa ctgctgcaac tgatcgcggt cgccgtcgat ggtgatggca tcctcgtcgc
      361 cgagcaggat cgtgtggcgg ctgctcatcg agcgcgcctg ctggtagacg gtgagtagca
      421 ggctatccaa ctccaccggg cggcggtcca gcggcgcccc ggcctgctcc atctcgccca
      481 gttgggccag gaacagcagg ttatcgacca gccggctcat gcgcgcggct tcgtcctcga
      541 tcaggcgctc gatggtatgg cgttcctccg cgtcgtgcgt gcggcgcagg agatcgagat
      601 tgccgcggat caccgtcagc gggctgcgca actcgtgcga cgagtcggcg acaaactggc
      661 gctgggcgtc gaaggcgacg gagatgcggt cgagcatgtt gttgaacgta cgcaccaggc
      721 ggcccatctc gtcgtcgccg gtggccgcgt cttccgggat gcgggcgccc aggctctgcg
      781 acagctcgat ggtgcgcgcg gtgtgcgtca cccggtccac cgcggccagg gtgcggctgg
      841 cggcaaacca gacgccgaag ccgatcaacg ccgaactgaa caacgtcgta ctgaccagca
      901 gcaggcgcat caaggccagc gcgctctggg tggacgccag gggcgtgccc acctccaccg
      961 ccagttgcac gggcgtcttg ttatccgggt cacgcaacgg cgcatagcgc acacgcatgg
     1021 cggtagtcgg gtccgcgcca atatcgcgcg tcaccatctc ccccttgagc gcctggtgaa
     1081 ttagcgtgcg gtcgatgggc agcaactccg gccctggggg atagaccttc tcgcctaaat
     1141 ggttcacgat ctgcacgtaa acggggacgc tctgggcacg ggtaggcggc aaatcaaagc
     1201 tattggcgac cggccccagc cgcaggtaat tgtcgatcac ctcggcgctg cggtcttcga
     1261 gggcggcatc gagctggcgg gcaaaggcgt cggcgacata gtggtacgac acgaagctga
     1321 tcaacgccag ggtcagcaac atcagcactg tggcgaacag gctcaagcgt agtttcagcg
     1381 acatgcgatt agcccttgtc ttcctcagac tcccgcaaca tatagcccac gccgcggacg
     1441 gtctggatga tgctgcccgc gcccaccttc tccagctttt gccgcaaata gcggatatag
     1501 acttccagga tgttgctctc gccgccgaag tcgtagcccc agatgcggtc gtagaactgc
     1561 ccgcgcgtca ccacctggcg cgggtggcgc agcatcagtt ccagcacgtc gaactctttg
     1621 ccggagacct ccagcgcccg cccgtcgtac accacttcgc gcgaggccgg cgtgacggtc
     1681 aggcggccca cccgcaacac ggcgggactg gtcgcggcgg cctggcggcg gcgcaacaac
     1741 gcgcgcaccc gcgccaggag ctcgtcgaag ctgaacggct tcaccaggta atcgtcggcg
     1801 ccgctgtcca gcccggccac tttatcgggc acgccgctgc gcgcggtcag cagcaagatc
     1861 ggcacgtcgc cgcccgcgcg caaccggcgg cagacctcca ggccgtccat gccgggcagc
     1921 atccagtcga gcacgaccag atccgggggt tggtcgcggg ccgcagcgag cgccgccgag
     1981 ccgtcttcga ccgccgccac cgaatagcct tcgtaggtca ggccgcgccg caataagtcc
     2041 aggatgcgcg gatcgtcgtc tactgcaaga atatgcatca tccctatcgg gcgtccggag
     2101 tacccaccct ctgccagccg cggatgagag aagctagccg tcggcgcgcc catcaccatc
     2161 ccaattatag cacaaacctc cggcttgccc gccgatactt atggcaggtt ttttcgttat
     2221 ataatggtaa gagaacaggg gcgagggcgg gacaggccca cccatccggg cctgttccgg
     2281 accgccgccg gtcgcccccc ttccgccgtg ccggcacgcg aggtgcgaac gcgatgccct
     2341 atccgagtta cctgaatatc gacgagtaca gcgcaatccg gcagaacggg tgcatccccc
     2401 tggcctggct ggcggcgctg cgtgacgcgc agccgctgga caccgaggcg ggcgagttct
     2461 ggggctggga gagcacgccc tgggaaacca cggacagcat cgaccgcgcg atcaatgtcc
     2521 tgcagcgcga cgaatacctc tgggcttatt tcaacattct cagcctgctg ctggaagaaa
     2581 tccagcaggt gcccaccgaa gagccgatca gcttcgacgt atccgaattc gccgccgtgg
     2641 acccggcacg cgcgcaggcc gcccgcgcgg cgagcgacaa tttccgcgcc atgctgcgca
     2701 tgctgcaccg gggcgagcgc gacgcggccc tggccgcgct gcgccaactc tccgccgacc
     2761 tcaatctcga cgccgggctg cccttcaccg gcgacctgga cgccgacatc gccgccatgg
     2821 gcggcggcga gaacgcgctg caagaactca cctggtcgat catgggcgaa atctacgagg
     2881 ggccgccgga gcgcgcggat tggtatacgc ccgagcatta ccggatcaac ttctggcatt
     2941 ggctggccgc ccccggcccg gcgtctgccc ctgaaggggc acctgcagag gcgccgccgg
     3001 ctgacgggac caccgggaac ggcagcgtga ttgccggcgt cgtcaatcac aacggatcag
     3061 caccggagct gtaaacctat ggctatcaat cctttccggg gtcccaggcc cccggacgct
     3121 gtcccccacc ccgcgggatc gctgggcccc gcgcgcccca accatggccg cctggcggcg
     3181 accctggcct atggcctccc ggctgccgcg ctggccgccg cgggcgccgg cctgtaccgc
     3241 cgcctccacc gctcggtgcc gcccatcgac ggcaccctgg cctgccctgt caccgcgccg
     3301 gtgcgcgtcg tccgcgacca ctggggcatc ccgcacatct acgcggagaa cgccgccgac
     3361 ctgttcacgg cccagggcta tgtgcaggcc caggaccggc tgtggcaact cgatttccag
     3421 cgccgggtgg ccgcgggccg cctgtcggag atcttcgggc cgcccacgct cgccaacgat
     3481 ctgctgctgc gccgcttcag cttgcgccag gccgccgaag ccgaagccgc ggccctgagc
     3541 gaggaagcca ccgccgcgct gacggcgtat tgcgccgggg tcaacgccta catcgcctgg
     3601 gcgcgcgagc accgcgcctt gcccgccgag ttcgccgtgc tggcctatga acccgaaccc
     3661 tggaccccgg cggatggcat ctcctggagc aaagtgatgg cctgggggct gggcggtaac
     3721 tgggaaagcg aactggtgcg cgcccggttg gtcaatcacc tcggccccgt gcgcgccgcg
     3781 gcgctggagc cggcctaccc gccgggccag ccgcttaccg ccgagccggg cgtcgccttc
     3841 tccggcctcg acgacctgtt cgcgcagttg ctggaacaat acgaagacct ggtccagatg
     3901 acgggcatcg gcgagatgat gcagagcgcc atcccggcca gcaacaactg ggtggtcagc
     3961 gccgagcgct cggccaccgg ccatgccctg ctggcgaacg acccgcacct gcccc
//
LOCUS       DASUYC010000297         6472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_943798,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000297 DASUYC010000000
VERSION     DASUYC010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6472
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943798"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..1760)
                     /locus_tag="VF276_04815"
     CDS             complement(171..1760)
                     /locus_tag="VF276_04815"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX6606219.1"
                     /translation="MIDPVRSDIQTAPAVRPAPATEGRARLAPPPPCAQASWLIARRE
                     IVDLLSDWRIIGPALLLVVVFPLLLVIMSAQGHDFLIRRVSPDAFTNLVPFALMLVGF
                     FPVSFSLMIALESFAGEKERNTLEALLSTPLSDRTLYLGKLLAALIPPLAASYLSMSL
                     YLGGVMLVTGYRPDWSLIGAIVLLDLAQALVMVGSALIISSHTTSVRAANLLAAFIIL
                     PMSVLIQGNNALILWGGGEQLWLIALALGLFALLLIRMGLRLFNREQILAREMDDLRP
                     GQLALTFRQFWALPPRAALQLRAVPIAASPVAPARPAGLGRLYRRDIPVLLRLRWPDL
                     ALVACALLFALALGWTLAERYPITTSTLINAAGSRGAAGGPLGLLGAVAGDDGGVLGL
                     LGGVVSGVLRVVLLSAFLIMLGVMSFGTLPVLFMMLVAGATGFVLGQLALAGTSPLVL
                     GITLLFPPVLFPALALLLLTAFAARFGLVVAAPPRGFSLGDSLLLALAEYLKICLLAV
                     PLLLLGGLLQSLLLALVQVVR"
     gene            2147..3112
                     /locus_tag="VF276_04820"
     CDS             2147..3112
                     /locus_tag="VF276_04820"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6606220.1"
                     /translation="MAVQAVTPARQVRPRANWGRRLLIAAVALAVLAALIVAGLSIYV
                     AGNLSSAYRVLVDPAVAASIGAKYSDVSFPSRQQVTLKGWLFHATNNTAGRSVIMVHG
                     WHHNRVDTSYHTDKIAQDLLQHGYDVLLFDLSANGDSGGDHFTLGTKEYLDVLGAYDF
                     LKSGAAGTKYDPHEMAVIGDSMGAASTLRASPEMPDVGAIVADSAFAELRPILEKELP
                     ARSPLPTFFTQPTLWAVPLYGLDPNLRPVDAVRAQPNRAFLFFHGLADDFVPPSNADE
                     LRAASTNPDTALVLVPGAKHVRTYEANPAAYMTRVYQFFDKEMSR"
     gene            complement(3265..4827)
                     /gene="malQ"
                     /locus_tag="VF276_04825"
     CDS             complement(3265..4827)
                     /gene="malQ"
                     /locus_tag="VF276_04825"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX6606221.1"
                     /translation="MNFERASGILLHPTSFPGPYGIGDLGAEAYRFVDFLQQTGQKLW
                     QILPLSPTGYGDSPYAAFSAFAGNPLLISPDLLCDQGLLQAKELKKVPDFPADRVDYG
                     PVIEWKMALLRRSYDEYKSGDHPAVRAELALFETESAGWLEDYALFMAGKTAHGGGVW
                     SDWDEGLALRQPEAMTEWRARLEDDIRFQKYIQYLFFKQWWTLKAYANERSVRIVGDI
                     PIFVAYDSADVWAHPELFYLDERGRTTVVAGVPPDYFSAKGQYWGNPLYRWDVLAANG
                     YAWWVERFRNVFRLYDFVRLDHFRGFAAYWEVPVNAEQTAANGRWVPGPGVPFFQAIR
                     AALGDQPIIAEDLGVITPDVVEIRQTMGFPGMRVLQFAFGAFETDATDPYLPHNFDHN
                     TVVYTGTHDNDTTAGWYETASEQERVNVRHYLDLDDRKDVTGQVIAEKLVRLAFSSVA
                     NTAIVPLQDLLGLGSEARMNLPGRAYGNWGWRYDPTCLTDAVRAHLRDITILFARYLP
                     PRDRPLKGQGNI"
     gene            complement(5082..5885)
                     /locus_tag="VF276_04830"
     CDS             complement(5082..5885)
                     /locus_tag="VF276_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6606222.1"
                     /translation="MAVQVDLYNDAYGNFAKAALAQVRRDTYGEDVGQSSWMTTGEYR
                     QFFAWLGLAPESLILDVGSGSGGPALFMARTVGCRVVGVDINENGIATANAQAQGVAD
                     QVRFQHIDAGHLLPFAEDTFDAIVCIDAILHIPDRVATLAEWWRVLRPGGRILFTDPV
                     IVTGFVTKEELATRSSIGYFLFAPVGADERAIQDAGLELIHREDVTDNAAQVSKRWHD
                     SRARHRADLISVEGETTYEGVQQFLAVVHGLTSERRLSRFVFVAQKSAR"
BASE COUNT         1191 a         2099 c         2160 g         1022 t
ORIGIN      
        1 acgcccttga gcgggaacat atgcaccgtg ctggtggtca gcacggtgcg cccggcggcg
       61 cggagttcga tcaacaggcg ttgcagcgtc gtcgtcttgc cgcccgcgcc gacgaaggcc
      121 accatctcgc cgggctggat atcgagcaca tgggtgaggg tgtgcatggg tcagcgcacc
      181 acctggacga gggccagcag caggctttgc agcaggccgc ccagcagcag cagcggcacc
      241 gccagcagac agatcttgag atattcggcc agggccagca gcaggctgtc gcccaggctg
      301 aagccgcgcg gcggggcggc caccaccagg ccgaagcggg cggcaaaggc ggtgagcagc
      361 aacagcgcca gcgcggggaa cagcaccggc gggaagagca gggtgatgcc gagcaccagc
      421 ggcgaggtgc cggccagggc cagttgcccc agcacaaagc cggtcgctcc ggccaccagc
      481 atcatgaaca gcaccggcaa cgtgccgaag ctcatgacgc ccagcatgat caggaaggcg
      541 ctcagcaaca ccacccgtag caccccgctc accacgccgc cgagcaggcc gagcaccccg
      601 ccgtcgtccc cggccacggc accgagcagc cccagggggc cgccggcggc gcctcggctg
      661 cccgcggcgt tgatcagcgt cgaggtcgtg atcgggtagc gttcggccag ggtccagccg
      721 agcgccaggg cgaacagcag cgcgcaggcc accagcgcca ggtcgggcca gcgcagccgc
      781 agcagcaccg ggatgtcgcg gcggtagagc cggcccagac cggcaggccg cgccggggcg
      841 acgggcgaag cggcgatggg taccgcgcgc aactggagcg ccgcgcgggg cggcagcgcc
      901 cagaactggc ggaaggtgag cgcgagctgg ccggggcgca ggtcgtccat ctcgcgggcc
      961 aggatctgct cgcggttgaa caggcgcagg cccatgcgga tcagcagcag cgcgaacagg
     1021 cccagcgcca gcgcgatcag ccacaactgc tcgccgccgc cccagaggat cagcgcgttg
     1081 ttgccctgga tgagcacgct catgggcagg atgatgaagg cggccagcag gttggcggcg
     1141 cgtacgctgg tggtgtggct ggagatgatc agcgcactgc ccaccatgac cagcgcctgc
     1201 gccaggtcga gcaacacgat cgcgccgatg agcgaccagt cgggccggta gccggtgacg
     1261 agcatgaccc cgccgaggta gaggctcatg ctgaggtagc tggccgccag cggcgggatg
     1321 agcgcggcca gcagcttgcc caggtagagc gtgcggtcgc tcaggggcgt ggagagcagc
     1381 gcttcgagcg tgttgcgctc tttctccccg gcgaagcttt cgagcgcgat catcagcgag
     1441 aagctgaccg ggaaaaagcc gaccagcatc agcgcgaagg gcaccaggtt ggtgaaggcg
     1501 tcgggcgaga cccggcggat caggaaatcg tgcccctggg ccgacatgat gacgagcaac
     1561 agcgggaaga cgacgaccag cagcagcgcc gggccgatga tgcgccagtc gctcagcagg
     1621 tccacgatct cgcgccgggc gatcagccag gacgcctgcg cgcacggggg cggcggggcc
     1681 aggcgcgccc ggccctcggt cgcgggcgcg gggcgcaccg ccggggcagt ctggatgtcg
     1741 gatcgtacag ggtcaatcat ggcggcacag ggcccccggc gcgctccgga gcggcggcgt
     1801 ggccccacag catggtgaaa tcagacatct acttccttac cattatacca tacgcgctac
     1861 ggcgactgct gcttgtgcac ccggcccccc gatgcgcgct gacggcgtaa cgccgaccgg
     1921 aatccggcat tttcatggag caggtttccc gctatggctt gcctgggaaa cccgtctgca
     1981 agcagtattg aaggtctagg cacccgaggc agattgtgat ataattaaca gggaccctca
     2041 tccggggtgc gcgcgctctt gcgcacccgg tcttgcctca gtgtgtcgct tgcgccatct
     2101 acagcgacca agacatgcat agctcatgcg aaacgaggta aatactatgg ctgtccaagc
     2161 agtcactccc gcccgccagg tgcggccccg cgccaactgg gggcgccgcc tgctcatcgc
     2221 cgccgtggcc ctggccgtgc tcgccgccct gatcgtggca ggcctgagca tctacgtcgc
     2281 cggcaacctg agttcggcat accgcgtact ggttgatccc gccgttgcgg ccagcatcgg
     2341 cgccaagtat agcgatgtca gcttccccag ccgccagcag gtgacgctga agggctggtt
     2401 gttccacgcg accaacaaca ctgccgggcg cagcgtgatc atggtccacg gctggcacca
     2461 caaccgcgtc gacaccagct accacacgga taagattgcc caggacctgt tgcaacacgg
     2521 ctatgatgtg ctgctgttcg acctgagcgc caacggggac agcggcgggg atcacttcac
     2581 gctggggacg aaagaatacc tggatgtgct gggggcgtat gacttcctga agagcggcgc
     2641 ggcgggtacc aaatatgatc cgcacgagat ggccgtgatc ggcgacagca tgggcgccgc
     2701 ctcgaccctg cgcgcctcgc cggaaatgcc cgatgtgggc gccattgtgg cagacagcgc
     2761 cttcgccgag ttgcgcccga tcctggagaa ggaattgccg gcccgcagcc ccctgcccac
     2821 cttcttcacc cagccgacgc tatgggccgt gccgctctac ggcctggacc cgaacctgcg
     2881 cccggtcgat gcggtacgcg cccagccgaa ccgcgctttc ctgttcttcc acggcctggc
     2941 tgacgacttc gtgccgccca gcaacgcgga tgagctacgc gccgccagca ccaaccccga
     3001 taccgccctg gtgctggtac cgggcgccaa gcacgtccgc acctacgagg ccaatcctgc
     3061 ggcctatatg acgcgggtgt accagttctt cgataaggag atgagccggt agcgcgcgtt
     3121 acgcagcgcg cgggccggtt tgaaaccggc ccctacagcg gcgaggggca tatccttgtc
     3181 actctgagcg cagcgaagag tctcgtctcg gttgggacaa gacgagatgc ttcgctgcgc
     3241 tcagcatgac ataggggcgc agcgctagat gttgccctgg cctttgagcg ggcggtcgcg
     3301 cggcgggagg tagcgggcga acaggatggt gatgtcgcgc aggtgggcgc gcaccgcgtc
     3361 ggtcaggcag gtcgggtcgt agcgccagcc ccagttgccg taggcgcggc ccggcaggtt
     3421 catgcgcgct tcgctgccca ggccgagcag atcctgcagc ggcacgatcg ccgtgttggc
     3481 gaccgaactg aaggccaggc gcaccagctt ttcggcgatg acctgcccgg tcacgtcctt
     3541 gcggtcgtcc aggtccaggt agtgccgcac gttcacccgc tcctgctccg acgccgtctc
     3601 gtaccagccg gcggtggtgt cgttgtcgtg ggtgccggtg tagaccacgg tgttgtggtc
     3661 gaagttgtgg ggcaagtatg ggtcggtggc gtcggtctcg aacgcgccga aggcgaactg
     3721 caacacgcgc atgccgggga agcccatcgt ctggcggatc tcgaccacgt cgggcgtgat
     3781 cacgcccagg tcttcggcga tgatcggctg gtcgcccagg gcggcgcgga tggcctggaa
     3841 gaacggcacg ccggggccgg gcacccagcg cccgttggcg gcggtctgtt cggcattaac
     3901 cggcacttcc cagtaggcgg cgaagccgcg gaagtggtcg aggcgcacga agtcgtacag
     3961 gcggaagacg ttgcggaacc gctcgaccca ccaggcgtag ccgttggcgg ccagcacgtc
     4021 ccagcgatag agcgggttgc cccaatactg gcccttggcg ctgaagtagt cgggcggcac
     4081 cccggccacc acggtagtgc ggcctctttc gtcgaggtag aacaattccg ggtgcgccca
     4141 cacatcggcg ctgtcgtagg cgacaaagat cgggatatcg ccgacaatgc gcacactgcg
     4201 ctcgttggca taggctttca gcgtccacca ctgcttgaag aacaggtact ggatatactt
     4261 ctggaagcga atatcgtcct ccagccgggc gcgccattcg gtcatcgcct ccggctgccg
     4321 cagggccaac ccctcgtccc agtcgctcca gacgccgccg ccgtgggccg tcttgccggc
     4381 catgaacagc gcgtaatctt ccaaccaccc ggcgctctcg gtctcgaaca gggccagttc
     4441 ggcccgcacc gcgggatgat cgccgctttt gtactcatcg taagagcgcc gcagcagggc
     4501 catcttccac tcgatcaccg ggccgtaatc gacccggtcg gcggggaagt cgggcacctt
     4561 cttgagctcc ttcgcctgca acagcccctg atcgcagagc agatcggggc tgatcagcag
     4621 cggattgccg gcaaaggccg agaaagcggc gtagggcgag tcgccgtagc cggtggggct
     4681 gagcggcaag atctgccaga gcttctggcc cgtctgttgc aagaaatcca cgaagcgata
     4741 cgcttccgcc cccaggtcgc cgatgccgta cgggccgggg aaactggtgg ggtgcagcag
     4801 gatgccgctg gcacgttcaa agttcacgtg acgccctcct tatacgcgag cagggttaaa
     4861 agacggctgc tgacctgctg cctgagagac tccccgcgga ttcacacgac tatcgcctgg
     4921 agtcgtcctg gccggtatcg ccgctggaat ggctcccgtc actacaatat ttgtgccctc
     4981 gtccccctga ttccgggcaa ttgttaaaga acgcgggcgc ggcggatggc gggccacaca
     5041 tgccatcatg gccgcaaggc gccaccggga accgcacata atcagcgtgc gctcttctga
     5101 gccacgaaga cgaaccgcga cagccggcgc tcgctggtga ggccatggac cacggccagg
     5161 aattgctgca cgccctcgta tgtcgtctct ccttccacag agatcaaatc ggcgcgatgg
     5221 cgtgcccgcg agtcgtgcca gcgtttggag acctgggccg cattgtcggt aacatcttcg
     5281 cggtggatca attcgaggcc ggcgtcctgg atcgcccgct catcggcccc caccggcgcg
     5341 aacaggaaat agccgatcga gctccgggtc gccagttcct ccttggttac gaacccggtg
     5401 acgatgacgg gatcggtgaa caggatgcgg ccaccggggc gcaagacgcg ccaccattcg
     5461 gccagggtgg cgacccggtc gggtatgtgc aggatcgcgt cgatacagac gatggcgtcg
     5521 aaggtatcct cggcaaaggg aagcagatgc ccggcatcga tgtgctggaa gcgcacctga
     5581 tcggcgactc cttgcgcctg ggcattggcg gtggcgatgc cgttctcgtt gatgtcgacg
     5641 cccaccaccc ggcagcccac cgtccgggcc atgaacagcg cgggcccgcc cgatccgctg
     5701 cccacgtcga ggatcagcga ctccggcgcc agccccagcc acgcgaaaaa ctgccggtac
     5761 tcgccggtcg tcatccaact gctctgcccg acatcctcgc cataggtgtc gcgtcgcacc
     5821 tgcgccagtg ccgccttggc aaaattgccg tatgcgtcat tatagaggtc tacctggaca
     5881 gccatggggg actcctttcc aacgctacaa caacaatgcg cgccgggatc gcgcggccgg
     5941 cggcggagcg atagcactca attgctggga atacgcgtgg gcagcgcgtg gcacctccag
     6001 aggcagactg agcggagcca ccacgcccta ttctagcaca ggtggggcgg cagcactaga
     6061 gtggcgcacg tgggacagga cgtgcggcgg gaatatcaac gcaaatgagc gagtacgagc
     6121 gggcttcgcg tacttaaccc gcggcttgag tcagcaggcc ccgccagcgt agaaatataa
     6181 agcgcactag gtgagatcga gaacatttac aaagtatgaa aagaaccgta tagtgcatta
     6241 ccgggcgtgt tgaaaaaggt gtctgcgaaa ccggattccc aggcacgcat gacgaacctt
     6301 atcgggcaca ctccttaatc gcagtcaggg agccgttttc gtggatcaag aaactagatg
     6361 gcgcatgtgt tacggttcgc cactggcaat aatggtgttt gcctttgccg tcgtcttttt
     6421 agtcggttgt ggtgctgccc ccgctgctgt gccactttcc gtgccaactc tg
//
LOCUS       DASUYC010000298         2290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1416495, whole genome shotgun sequence.
ACCESSION   DASUYC010000298 DASUYC010000000
VERSION     DASUYC010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1416495"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(314..1468)
                     /locus_tag="VF276_04835"
     CDS             complement(314..1468)
                     /locus_tag="VF276_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014814749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6606223.1"
                     /translation="MPATPPPPPPIRLGLQLSAAPPLALMRALVYWARLIGCDSCLVS
                     DSLQNFVPTALWDPQFTWAATQAPSAHIFLDWSVLLGDLARRAGRLHLGVGVTDPTRR
                     HPVLLAQAILTLAHLTHHAPILGLGAGERLNVEPYGLDFTTPVGRLEEALQIIRACLA
                     PHEGPITFHGRHYHLADAALDLRAPAGKTPRIWIAARGPRMLRLTGLYGDGWYPALIA
                     SPQEYAAKLQVVRDAARAAGRNPAAITPALYQAVLVAPTARGVAALRNSTTGRLGGMY
                     AAAAAWRRFGVAHPFGADYHDLLLDFLPHRYDRATCERGLATVPPALRGYGYLYGTPE
                     QIAAKLHAFGAAGMRHVVLDLIGQVTISARAGLYGLLAARRIARLLRSGP"
     gene            complement(1805..>2290)
                     /locus_tag="VF276_04840"
     CDS             complement(1805..>2290)
                     /locus_tag="VF276_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606224.1"
                     /translation="YGLNVSAGRARGLAAGAAATALVSLALYGVLQAADGVGLKHAVD
                     AWAAAPAAEKAARFASAETMRWLEWAARGYHAFVLGLALLLYAGAIMGTGRLPRGLGA
                     VLGLCGLAYWVQGWVVSAEGFSPANSLPTLLTIVLFLAGSLWLLIWLLRGKDARAALP
                     N"
BASE COUNT          375 a          757 c          873 g          285 t
ORIGIN      
        1 tgccaaaact gtgtgccttc catcgacctg acctcccaac aatatgccgg ccgcgctgcg
       61 gggggacagg aacagcgcca tgcggacggg cgaccgtccc cgcgtcatca ttgtagcagg
      121 tttaccccga ggcagcactt ccgcgcccgc cgcagggtgc gggcagagta cggggacgta
      181 tgtggggacg caatatcggc aagtgcgaac gtggcggggc ggcaaggagt ctgcctccgt
      241 gctacccggt ccccgcgcgt gggccgaact ataggtcttg tagtataggc gtggaggcgc
      301 acaagccggg tcgtcatggc ccgctgcgca acaaccgggc aatccggcgt gccgccagca
      361 ggccatagag tccagcccgc gccgaaatcg tcacctggcc gatcagatcc agcaccacgt
      421 ggcgcatccc ggcggcgccg aacgcgtgga gcttggccgc gatctgctct ggggtgccgt
      481 agagatagcc gtagccgcgc agggcgggcg ggaccgtggc tagcccgcgc tcacaggtgg
      541 cgcgatcata gcggtggggc aggaagtcca gcagcagatc gtgatagtct gcgccgaagg
      601 ggtgggccac gccgaaccgg cgccacgccg cagccgcagc gtacatgccg cccaggcggc
      661 ccgtggtcga gttgcgcagg gccgccacgc cccgcgcggt gggcgccacc aggactgcct
      721 ggtagagcgc cggagtaatc gcggcgggat tgcggccggc ggcgcgggcc gcgtcgcgca
      781 caacctgcag cttggcggcg tactcctgcg gcgaggcgat gagggccgga taccaaccat
      841 cgccgtacag cccggtcagc cgcagcatgc gcggtccgcg cgcggcaatc cagatgcggg
      901 gcgtcttgcc cgccggggcg cgcagatcca gcgcggcgtc cgcaaggtgg taatggcggc
      961 catggaaggt gatcggtcct tcatgggggg ccaggcaggc gcggatgatc tgcagggcct
     1021 cttccaggcg gcccaccggg gtggtgaagt ccaggccgta gggttcgaca ttgaggcgtt
     1081 cgcctgcacc caggccgagg atcggggcgt ggtgggtgag gtgggccagg gtgaggatgg
     1141 cctgggcgag cagcaccggg tgacgacggg tggggtcggt gacgccgacg cccaggtgca
     1201 gacgcccggc gcggcgggcg aggtcgccca ggagcaccga ccagtcgagg aagatgtggg
     1261 cactgggggc ctgggtggca gcccaggtga actggggatc ccagagcgcg gtggggacga
     1321 agttctgtag gctatcggag accaggcagg aatcacagcc gatgaggcgg gcccagtaga
     1381 cgagggcgcg catcagcgcg aggggcgggg cggcggagag ttgcaggccg agcctgatcg
     1441 gcggcggcgg gggcggcgta gcgggcatag cagtatcttc cctatgggca gggcgggccg
     1501 ggtggaaggc gcgagcaccc ggcccgtgac cccgcgcaat ccaagctacg gcgccgcagg
     1561 ggcgcacagg ccggaaggaa gggcgggcgt ggtgctatta tagcagatgt tacacatgag
     1621 taagcgcgcc ggccggtggg ggcgtttgtc agccggcgcg gattgcaagc cagtggtccc
     1681 tggcgacggg gaggagaaga cgaccgccag ggtggcgggc gctggccggg cgccgctttc
     1741 ctggcgcgag cggcgcctac cgggcggggc gggagtcgtc gtcccccgtc ccactctgcc
     1801 agggctagtt cggcaaggcg gcccgtgcgt ctttcccgcg tagcagccag atcagcagcc
     1861 agagactccc ggcgaggaac aggacaatgg tcagcagcgt gggcagactg ttcgcggggg
     1921 agaagccctc tgcgctgacg acccagccct ggacccagta ggccaggcca cacaggccca
     1981 gcaccgcgcc caggccccgc ggcagccgcc ccgtccccat aatggccccg gcgtacagca
     2041 gcagcgccag gcccagcacg aaggcgtggt agccccgcgc cgcccactcc agccagcgca
     2101 tcgtctcggc actggcaaac cgcgcggcct tctccgccgc cggtgccgcc gcccaggcat
     2161 cgaccgcatg cttgagcccc actccgtccg ccgcctgcag caccccatac agcgccagcg
     2221 acaccagcgc ggtcgcggcc gcgcccgccg ccagcccgcg ggcgcggccg gcggacacgt
     2281 ttagcccgta
//
LOCUS       DASUYC010000299         2444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1938626, whole genome shotgun sequence.
ACCESSION   DASUYC010000299 DASUYC010000000
VERSION     DASUYC010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1938626"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1364
                     /locus_tag="VF276_04845"
     CDS             <1..1364
                     /locus_tag="VF276_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606225.1"
                     /translation="QSALAPAAAGVAWLFLRDRRAGLRFGGVLLGLLVAGFVALLALS
                     RGGFWEHAIVYQTFPWTLDYWRRLMGRLSGEYGALTLIGVLVALWGLGGWLRRLRGAN
                     LLRDNLELSLPVLYALAATGSLMIQAGYAEGNYNHLLDIFPPLIWLLGRALAGARTAR
                     TAPQWALVAGLYGLLLIQVWLPQGPERWYSMIYWPSPARTTEMTRLGALLRDTPGEIY
                     TEDATVVLLTGRPLAYDDPDTIAAMGQRGLWDQSRVVQDLRDRRFPLIFMRHGAYRWS
                     RDGLAAFDDSYEPAFQGSIDIYRPRVYPLGPQYTLGCEMGSPAELRLAGVSLGPGVAA
                     DGIAAGSDLRLALYWDALRPPGDYATFVHLIDAGGQTVAARDNPHSATDRPTSTWAPG
                     TSVTEITDLPLPAGLAPGTYRLIGGVYSTAGGQFQSVPVACPAAPERRVGDAVDLGAV
                     PVR"
     gene            1389..>2444
                     /locus_tag="VF276_04850"
     CDS             1389..>2444
                     /locus_tag="VF276_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606226.1"
                     /translation="MTDLRILANRLPGWAIPRTPAWAARAEAAALPSWRAALRFAILA
                     VLAVRLLITLWAAVDLLVQPADQAALERQYAIWHAPLYGEGVAGLLLGPWQREDSLRY
                     EQIAARGYQAGQGDIVLFPLFPLLLRVVGVLTLGNYAVAGALVSLLATIGAVAVLYRL
                     IQIDFNRQVAERTVAYLLLFPVAYYLLIPYAESVFLALVCATFYAVRRGHWAWTALFA
                     FLAALTRLQGVLLAIPIGIEALAVAGWQIRAIPWRKVLPAAVAPVLGLAAEQAFNTWI
                     VRDAHSWLEWQAAFGGGKYTTWPWVALAGSLQTVLGERGITVNTFDFAVAVLFLALLV
                     LCMRYLRPSYTLWSAAMV"
BASE COUNT          294 a          856 c          860 g          434 t
ORIGIN      
        1 agcaatcggc gctggccccg gctgctgcag gcgtggcctg gctgttcctg cgcgaccggc
       61 gggccgggct gcgcttcggc ggcgtgttgt tgggcctgct ggtggccggg ttcgtggcgc
      121 tgctggcgct ctcgcggggc ggcttctggg agcacgccat cgtctaccag accttcccct
      181 ggacgctcga ctactggcgg cggctgatgg gccggctcag cggcgaatac ggcgcgctga
      241 ccctgatcgg cgtgctggtg gcgctgtggg gcctgggcgg ctggctgcgc cgcctgcgcg
      301 gggccaacct gctgcgcgac aacctggagc tgtcgctacc cgtgctctac gccctggccg
      361 ccaccggttc gctgatgatc caggcgggct atgctgaagg caactacaac cacctgctcg
      421 acatcttccc gccgctgatc tggctgctgg ggcgggcgct ggccggggcg cgcacggccc
      481 gcaccgcgcc gcagtgggca ctggtggccg ggctctatgg gctgttgttg atccaggtct
      541 ggttgccgca aggcccggaa cgctggtaca gcatgatcta ctggcccagc ccggcgcgga
      601 ccaccgagat gacccgcctg ggcgccctgc tgcgcgacac gcccggcgag atctacaccg
      661 aagacgcgac cgtggtgctg ctcacgggcc gcccgctggc ctatgacgac ccggacacca
      721 tcgcggccat gggccagcgc gggctgtggg accagagccg tgtggtgcag gacctgcgcg
      781 accgccgctt cccgctgatc ttcatgcgtc acggcgccta tcgctggagc cgggacgggc
      841 tggccgcctt cgacgacagc tacgagccgg ctttccaggg cagcatcgac atctatcgcc
      901 cgcgcgtcta tccgctgggt ccgcagtaca cgctcggctg cgagatgggc agcccggcgg
      961 aattgcgcct ggcgggcgtc agcctggggc cgggcgtggc cgctgacggt atcgcggcgg
     1021 gcagcgatct gcgcctggcg ctctactggg acgccctgcg cccgcccggc gattatgcca
     1081 ccttcgtcca cctgatcgac gcgggcggcc agaccgtcgc cgcccgcgac aatccgcaca
     1141 gcgctaccga ccggcccacc tcgacctggg cgcccggcac ctccgtcacc gagatcaccg
     1201 acctgccgct gcccgccggc ctcgcgcccg gcacctaccg cctgatcggc ggcgtctaca
     1261 gcaccgccgg ggggcagttc cagtccgtgc cggtggcctg ccccgccgcc cctgagcgcc
     1321 gggtgggcga tgccgtggac ctgggcgccg tcccggtgcg ttagccggct gacatagaag
     1381 gaatgttgat gaccgatctg cgaatcctgg cgaaccgtct ccctggttgg gccatcccgc
     1441 ggacgccggc ctgggccgcc cgtgcggagg ccgcagcctt gccgagctgg cgcgccgcac
     1501 tgcggttcgc gattctcgcc gtgctggccg tgcgcctgct gatcaccctc tgggcggcgg
     1561 tggatctgct tgtccagccc gcggaccagg ccgcgctgga gcggcagtac gctatctggc
     1621 acgccccgct gtacggggag ggggtcgccg gtctgttgct cggcccctgg cagcgggaag
     1681 atagcctgcg ttacgagcag atcgccgccc gcggctacca ggccggacag ggcgacatcg
     1741 tgctgttccc gctgttcccg ctgttgctcc gcgtggtggg cgtgctgacc ctgggcaact
     1801 acgctgtggc gggcgcgctg gtgagcctgc tcgcgacgat cggcgcggtg gctgtgctgt
     1861 accggctgat ccagatcgat tttaaccgcc aggtggccga gcgaaccgtc gcctacctgc
     1921 tgctctttcc ggttgcctac tatctgctca ttccctacgc cgaatcggtc ttcctggcgc
     1981 tggtctgcgc cacgttttac gccgtgcgac gcggacactg ggcgtggacg gcgctgttcg
     2041 ctttcttggc ggcactcacc cggctgcagg gcgtgctgct ggcgatcccg attggcattg
     2101 aggcactggc cgtcgccggg tggcagatcc gggcgattcc ctggcgcaag gtactgccgg
     2161 ccgccgtcgc acccgtgctg gggctggccg ccgagcaggc gttcaacacc tggatcgtcc
     2221 gcgatgccca ttcctggctg gaatggcagg ccgctttcgg cgggggcaag tatacgacgt
     2281 ggccctgggt agctctggcg ggcagcttgc agacggtact cggcgaacgc gggatcaccg
     2341 tcaacacctt tgatttcgcc gtggccgtgc tgttcctggc actgctggta ctctgcatgc
     2401 gttacttgcg accaagctat acgctctggt ccgcggcgat ggtg
//
LOCUS       DASUYC010000300         3687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_544659,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000300 DASUYC010000000
VERSION     DASUYC010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544659"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /gene="mtnA"
                     /locus_tag="VF276_04855"
     CDS             complement(<1..755)
                     /gene="mtnA"
                     /locus_tag="VF276_04855"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEX6606227.1"
                     /translation="MHLTRTLWWEEGALVLIDQRQLPSAYETLRCTTPEEVATAIRDM
                     AVRGAPAIGCAAAYGLALSAAHHADAGLTAQRAALQADAALLRATRPTAVNLAWALDR
                     QLALARAYRGQDPAELAAHLLAEAEQIADDDEAACRTMGAHGAALIPPGGQVLTHCNA
                     GSLATAAYGTALGVVRAAWEQGRLAHVLVDETRPRLQGARLTAWELQQAGIPCTLIAD
                     NMAASFMRRGAVDAVVVGADRIAANGDTANKIGT"
     regulatory      complement(807..936)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(1092..2177)
                     /locus_tag="VF276_04860"
     CDS             complement(1092..2177)
                     /locus_tag="VF276_04860"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX6606228.1"
                     /translation="MSTEGPDTPPDRAERAFAHLPHRTQIARMRQVAVAALAAYDLPP
                     VRVRLLAHRFNTTFHVDTEGGARYVLRINRAGAPTVETVGSELAWLAALRRDTALEVP
                     APVATRAGALLTVADAPGVPHPHICVLFRWMPGHLSRHGLTPRRMERVGVLMAQLQDH
                     ADHWERPPGFTRGRVDWPVAMGRTLPDPYGPEVVAYIEGLVAETLSAVEAAPVTEALA
                     RVRAAVQALGQGPDAFGLIHADLHYGNLLFSGDTVRAIDFDDCGFGFRLFDFGVMLSA
                     VLDWPAYPALRAALLAGYRRVRPLPAAHEAHLDAFIALRLVQDALWILEWRRHPALGA
                     DWAGQAREVLATLAAFLATGSVAPAGS"
     gene            complement(2222..2740)
                     /locus_tag="VF276_04865"
     CDS             complement(2222..2740)
                     /locus_tag="VF276_04865"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HEX6606229.1"
                     /translation="MDTKQFTNPSMIAGIGLIVLGLIFFAVTQGAFNLDWGNIWPAFV
                     VLAGLYLLAQAFSSNNASRRANGIMAGTLVTLLGAFFFATTFGIVSWGLWPMYSLIVA
                     AAFLAGYFASGREATRFLMPAAILGLIGIVFLALDATGAGYGAIGRLWPIFLVIAGVL
                     VLAQGARRPRAG"
     gene            3028..>3687
                     /locus_tag="VF276_04870"
     CDS             3028..>3687
                     /locus_tag="VF276_04870"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX6606230.1"
                     /translation="MFNTWRAISRRTLGVLLLGLFLPAALPALPGRAAPAPLVVPDWQ
                     DTGITLPELHLPLCFDATRPNVVLVADDSAGTVAFDWTTGTRTVVNSRPFTLCGPNGL
                     LFAGDRPAGPAWRFSLDDPTGQAIAHLPGRAAQDGSNRVYALELVSPTGPAARLWASA
                     DGGLTWEPKNGPPTGSLESIAVAPADGRLLYAVAVEVAPGGVTYVIYASNDGGTTWEE
                     RA"
BASE COUNT          613 a         1335 c         1230 g          509 t
ORIGIN      
        1 gtaccgattt tgttggccgt atcgccgttg gcggcgatgc ggtccgcgcc gaccacgacc
       61 gcgtccacgg cgccccggcg cataaaactg gccgccatgt tgtcggcgat caacgtgcag
      121 gggatgcccg cctgctgcaa ctcccaggcg gtgagccggg cgccctgcag gcggggccgc
      181 gtctcgtcga ccagcacatg ggccaggcgg ccctgctccc acgcggcgcg aaccacgccg
      241 agcgccgtgc cgtaggccgc cgtcgccagg ctgcccgcgt tgcaatgggt cagcacctgg
      301 ccgccgggcg ggatcagcgc cgcaccgtgc gcgcccatcg tgcggcacgc ggcctcgtcg
      361 tcgtctgcga tctgctcggc ctccgccagc aggtgcgcgg ccagttccgc cgggtcctgc
      421 ccgcggtacg cccgcgccag cgccagttgc cggtcgagcg cccaggccag gttgacggcc
      481 gtaggccgcg tggcgcgcag cagggcggcg tccgcttgca aggccgcccg ttgcgccgtc
      541 aggccggcgt ccgcgtggtg ggcggcgctc agcgccagcc cgtaggcggc agcgcagccg
      601 atggccgggg cgccgcgcac ggccatatca cggatcgccg tggcgacttc ctccggcgtg
      661 gtgcagcgca gcgtttcgta cgcgctcggc agttgccgct ggtcgatcag gaccagtgcg
      721 ccctcttccc accagagggt tcgtgtcaga tgcaaaaaaa atctcccatc gcattacaca
      781 atgagagaat agccgatggg gccgctctct catctttcag cagcagacgc cggagcttgc
      841 tgacggattt ggcacccatg actgtgccgc gcttgcggat cacagccggg ttgccgggct
      901 tcacagggcc ggtccctcca cccctctcga tgagtaacga gctattcact tggaaacgcg
      961 gcgccgattg cgcatatttg agacacaaca aacgccgtgc gttcatacac agcttgctaa
     1021 taatagcata ttggtccggt ctgtgtcaag aaatcgcgca aacttccggc gcgagagcca
     1081 ggcagggtgc ctcaagagcc ggcgggcgcc acgctgcccg ttgccagaaa ggccgccagc
     1141 gtcgccagca cttcgcgcgc ctggcccgcc caatcggcgc cgagcgccgg gtgccggcgc
     1201 cactccagga tccagagcgc gtcttgtacc aggcgcaaag cgataaaggc atccaggtgg
     1261 gcctcgtgtg cggcgggcag cgggcgcacc cggcggtagc ccgccagcag cgccgcacgc
     1321 aacgcgggat aggccggcca gtcgagcacg gcactcagca tcacgccgaa gtcaaacagc
     1381 cggaagccaa agccgcaatc gtcgaagtca atggcccgca ccgtgtcgcc ggaaaacagc
     1441 aggttgccgt aatggagatc ggcgtggatc aggccgaagg cgtccggtcc ctggcccagc
     1501 gcctgcaccg ccgcccgcac ccgcgccagg gcctccgtca cgggggcggc ctccaccgcc
     1561 gagagggtct ccgccaccag gccctcgatg taggcgacga cctcggggcc atacggatcg
     1621 ggcaacgtgc ggcccatggc gacgggccaa tcgacccggc ctcgcgtaaa gcccggcggt
     1681 cgctcccaat ggtcggcgtg atcctgcaac tgcgccatca gcacgcccac gcgctccata
     1741 cggcggggcg tcaggccgtg ccgcgagagg tggccgggca tccagcggaa cagcacgcag
     1801 atatgcggat gcggcacgcc gggcgcatcg gccaccgtga gcagcgcccc ggcccgcgtc
     1861 gccacgggcg cgggcacttc gagtgccgtg tcgcgccgca gggcggccag ccaggccagt
     1921 tcggatccga ccgtttccac cgtcggggcg cccgcccggt tgatgcgcag gacgtagcgg
     1981 gcgccgccct ccgtgtccac gtggaaggtg gtgttaaagc gatgggccag cagccgcacg
     2041 cggaccggcg gcaggtcata ggcggccagg gccgccacgg ccacctgccg catccgcgcg
     2101 atctgcgtgc ggtgtggcag gtgcgcaaac gcccgctccg cgcggtcggg cggtgtgtcc
     2161 ggtccctctg tgctcaacgc aggtcgcctg gcctgcgggc ctcgtcagac actggcacag
     2221 gtcagccggc gcgcggccgg cgcgccccct gggcgagcac cagcacaccg gcgatgacca
     2281 ggaagatggg ccacaaccgg ccgatggcgc cgtagccggc cccggtcgcg tccagagcca
     2341 ggaagacgat gccgatgagg cccaggatcg ccgccggcat caggaagcgc gtggcttcac
     2401 gcccggaagc gaaatacccg gccaggaacg ccgccgccac gatgagcgag tacatcggcc
     2461 agagccccca ggagacgatg ccgaaggtgg tggcgaagaa gaacgcgccc agcagggtca
     2521 cgagcgtgcc cgccatgatg ccgttggccc ggcgcgacgc attattgctg gaaaaggcct
     2581 gggccagcaa atacagcccg gccagcacga caaaggccgg ccagatgttg ccccaatcga
     2641 gattaaacgc cccttgcgtc accgcgaaaa agatcagccc cagcacgatg aggccgatgc
     2701 cggcgatcat actcggattc gtgaactgct ttgtgtccat cgcgcccctc ctcggctata
     2761 cattcagacc tacagaccta agcatacttt gcgcgggtcg gcgaagcacc atgcaaaagt
     2821 cgcctttccc tatccgcctc tagttggatt ataccacctg gcgccgcagt gtgctggccc
     2881 cggcgcacag gaaacccgcc gcgcccgccc gcgttaatac aggtagaaga tccgcaacaa
     2941 ggcaccgaca accattagat gatcccgtca cgccgggtcg agccggcgac cagggccgcg
     3001 cattgcgctt ttcaaggaag gacgcatatg ttcaacacct ggcgcgccat tagccggcgc
     3061 acgcttggag tgctcctgct cggcttgttc ctgccggccg cattgccggc cctgcccggc
     3121 cgggcggccc cggccccgct ggtcgtgccc gactggcaag acaccggcat cacgttgccg
     3181 gagctgcacc tgccgctctg cttcgacgcc acccggccca acgtggtgct ggtggccgac
     3241 gacagcgcgg gcactgtggc cttcgactgg accaccggca cgcgcaccgt cgtcaacagc
     3301 cgcccgttca ccctctgcgg ccccaacggt ctgctgttcg ccggggatcg gcccgcgggg
     3361 cccgcctggc gcttcagcct ggacgacccg accgggcagg ccatcgctca cctgccgggg
     3421 cgcgccgccc aggacggcag caatcgcgtc tacgcgctgg aactggtttc gccgaccggg
     3481 ccggcggccc gcctctgggc cagcgccgac ggcggcctca cctgggaacc aaagaacggg
     3541 ccgccgaccg gcagcctgga gagcatcgcc gtggcccccg ccgacggccg gctgctctac
     3601 gcagtggccg tcgaagtggc gcccggcggc gtcacctacg tgatctacgc ctccaacgac
     3661 ggcggcacca cctgggagga acgcgcc
//
LOCUS       DASUYC010000301         3877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1050732, whole genome shotgun sequence.
ACCESSION   DASUYC010000301 DASUYC010000000
VERSION     DASUYC010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050732"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VF276_04875"
     CDS             <1..402
                     /locus_tag="VF276_04875"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606231.1"
                     /translation="TGSDDKTARLWSAKTGQLLQTFTETSRVWGVAFSPDSRRALLAL
                     ADGTGRILDLTTGVEVRRLLGHTNSLLSGVFSPDGKLVLTTSADMTARVWQVDYRDSL
                     QALCARLTRDFSDAERAKYEIADKTPTCPGP"
     gene            complement(422..2356)
                     /locus_tag="VF276_04880"
     CDS             complement(422..2356)
                     /locus_tag="VF276_04880"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6606232.1"
                     /translation="MDGPASFGAWVRQQRKALDLTQDLLAERAGCSSEMIRKIEAGRA
                     RPSRQLAELLVRALGVSVADRERVIGWARTVSPTPVPTAKVVDGASAPPMETPLTNLP
                     TSLTALVGRDPELAQSLALLAHPDVRLLTLTGPPGVGKTRLALQVASNLQINFAGEVW
                     FIALAAVTDVETLIAAIAGTLSVRPEPGRPLLPTVQTAIRGRQLLIVLDNFEHIAAAG
                     ASVLGDLLGGAPDLKLLVTSRVRLHLRGERELALAPLQLPPAAAPLNPSVLAGVPAVA
                     LFVERAAEAQTGFMLTMENAPAVAAICRRLDGLPLALELAAARSKILSPAALLARLDT
                     RLSLLTYGAGDLPAHQQTLRDTITWSYNLLDPATQELFCCLAVFAGGCTLAAAEAICA
                     PANGTAGQVLLHPAPVPPAEVLEGIAQLVDQSLLQRTDDPTGETRFTMLETIHEYAEE
                     RLRESTDAAEVRERHATYYLALAEAADRAWSGPEQMEWAERLEVEHDNLRAALDWAEE
                     TGRRETGARLAGALARFWATHGFLDEARRRLAWALQPGSTLPAPLRARVLDAASEIAY
                     WQADYAAARAWAEDGLSLRRALGQPVQIADALTNLGRILWRLGDPAAAQALLTESQVL
                     RRALGTSPGSAETRHPSTEV"
     gene            2523..2834
                     /locus_tag="VF276_04885"
     CDS             2523..2834
                     /locus_tag="VF276_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606233.1"
                     /translation="MERFNKHIRTVIVVLGVAAGTALSIGSVGAAYPTNTSVAIPATP
                     ALPRVARIPAMCLDDGPHLEGGILAWALTTGIKDSRHYWYHQRALADAYWAYYACPDA
                     S"
     gene            3133..3402
                     /locus_tag="VF276_04890"
     CDS             3133..3402
                     /locus_tag="VF276_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606234.1"
                     /translation="MNVSIIFGNKAVRATLAGVLAGLAILAPGSGAVGRATPRIDPPN
                     ATAYSYYYHYAWYDHPEPPSAAPMNLGDPLIGPGIPMDQDLRALV"
     gene            complement(3666..>3877)
                     /locus_tag="VF276_04895"
     CDS             complement(3666..>3877)
                     /locus_tag="VF276_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606235.1"
                     /translation="VGSNGTDLQYVERGIFERAAATARELLGDDVYAAARSAGQAMTI
                     EQAVAYAQDGLVPAGLEVPLLIHSR"
BASE COUNT          705 a         1260 c         1245 g          667 t
ORIGIN      
        1 accggcagcg acgacaagac ggctcggctg tggagcgcga agacggggca actcctgcaa
       61 accttcaccg aaaccagccg cgtgtgggga gtggcgttct cgcccgatag ccggcgagcc
      121 ttgctggccc tcgccgacgg aacggggcgg atcctggatc tgacgaccgg cgttgaggtg
      181 cgccggcttc tcggccacac gaattccctg ttatccggtg tgttttctcc agacggcaaa
      241 ctggtgctga caacgagcgc ggacatgacg gctcgtgtct ggcaagttga ctaccgcgac
      301 tcgctgcagg cgctgtgcgc ccgccttacg cgcgacttct cggacgctga gcgggctaaa
      361 tacgagatcg cagataagac cccgacctgt ccaggcccgt agtcccagcg cgcaaccggt
      421 gtcacacctc agtagaggga tgccgggtct cggcgctacc gggactcgtg ccgagcgcgc
      481 gccgtagcac ctggctctcg gtcagcagcg cctgggcggc ggcggggtcg cccaggcgcc
      541 agaggatgcg ccccaggttg gtgagggcgt cggcgatctg aaccggctgg cccagggcgc
      601 ggcgtaggga cagtccatcc tcggcccagg cgcgggcggc ggcataatca gcctgccagt
      661 aggcaatctc actcgccgca tccagcacgc gggcgcggag cggcgctggg agggtgcttc
      721 ccggctggag cgcccaggcc agccggcgtc gcgcctcgtc caggaagccg tgcgtcgccc
      781 agaatcgtgc cagcgccccc gccagccggg ccccggtctc gcgccggccc gtttcctctg
      841 cccaatccag cgcggcgcgc aggttgtcgt gctctacttc caatcgttca gcccactcca
      901 tctgctcggg gccggaccag gcccgatcgg cggcttcagc cagggccagg taataggttg
      961 cgtgtcgctc acggacctcg gcggcatccg tgctctcccg cagccgctcc tctgcatatt
     1021 cgtggattgt ttcgagcatg gtaaagcgcg tctcgccggt agggtcgtcg gtgcgctgta
     1081 acaagctttg atcgaccagc tgggcgatgc cttcgagcac ctctgcgggc gggacgggag
     1141 ccgggtgaag gagcacctgg cccgccgtgc cattggccgg cgcacagatc gcctctgccg
     1201 ccgccaacgt acagcccccg gcgaacactg cgaggcagca gaacagttct tgggtggcgg
     1261 gatcgagcag attatagctc cacgtaatgg tatcacgcag ggtttgctga tgagccggca
     1321 gatcgcccgc gccatacgta agcaacgaga gccgagtgtc cagtcgggcc agcagggccg
     1381 cgggcgacag aatcttgcta cgtgccgcag cgagttcgag tgccagtggc aacccatcca
     1441 ggcggcggca aatcgcggcg acagcggggg cgttctccat ggtgagcatg aagcctgttt
     1501 gcgcctcggc ggcacgctcc acaaagaggg caacggcagg caccccagcc aggacagacg
     1561 gattgagtgg cgccgccgcc ggtggcaact gcaagggcgc cagggctagt tcgcgttcgc
     1621 cgcgcaggtg caggcgcacc cggctcgtga ccagcagctt caggtcgggc gctccgccga
     1681 ggagatcccc aagcacgctc gcgcctgcgg cggcgatgtg ctcaaagttg tccagcacga
     1741 tcagcaactg ccggcctcgg atggcggtct ggacggtggg caggagtggc cggcccggct
     1801 ccggacgcac actcaacgtg ccggctatgg ccgctatcag ggtttcgaca tcggtaacag
     1861 ccgcaagcgc gatgaaccag acctcgccgg caaaattaat ctggagattg gaggcgacct
     1921 ggagagcgag acgggttttg ccgacgcccg gcgggccagt gagggtaagc aaccggacgt
     1981 cggggtgtgc aagcaaggcc aggctttgcg ccagttccgg atcccggcct acgagtgcag
     2041 tgaggctagt gggcaggttg gtgagtggag tctccatggg cggtgcactc gcgccatcga
     2101 ccaccttcgc cgtgggcacc ggagtgggcg agacagtgcg cgcccagccg atgacccgct
     2161 cccgatccgc cacggacaca ccgagggcgc gcaccaggag ttcggccaac tgccgcgagg
     2221 ggcgggcgcg acctgcctcg attttgcgaa tcatctcgct ggagcaaccg gcccgctcgg
     2281 ccagcagatc ctgggtcaga tcgagcgcct tacgttgttg ccggacccac gccccaaagg
     2341 atgccggacc gtccatcggc tctctccgtt cccggttgct ttcccggtta ccttcccgcc
     2401 tattatacca cttttccctt cttggaatag ccgctttctg gttcaatagc ctcacacaaa
     2461 cacgcaccga gaatacgagc cgacggatat acaaaaccct aaactaagaa aggcgcacta
     2521 cgatggaacg gttcaacaaa catatccgca ccgtaattgt ggtcctaggc gtggcggcgg
     2581 gcactgccct ctcaatcggc agcgtggggg ccgcgtatcc cacgaatacg agtgtcgcta
     2641 ttccggccac cccggccctg ccgcgggttg cacgcatccc agccatgtgc ctcgacgatg
     2701 gtccccacct ggaaggcggg atcctggcct gggccttgac gacgggcatc aaggattcgc
     2761 gccattactg gtatcaccag cgggccctgg cggatgccta ttgggcgtac tatgcctgcc
     2821 ccgacgcctc ctgactcggt gcccagtgcg gtgcccgcca agatccggct cggtcattcc
     2881 aggtactcgg gttccgttaa gactataccg aaacgcggct gctcccactt gttgcatcgc
     2941 atccttatgc gacgctagtc aaataccagc gtttactgcg cttcacgctg gtcctgaccg
     3001 ttttgggaac gctgggggaa cgggcggttt gctaccatga cagcagcaga attcacaagc
     3061 cgaatgagtg gtggggcctg gcttaatcac ctgacaacga accagtacca agaaaatgaa
     3121 aggagatccc cggtgaacgt atccatcata ttcggcaaca aagcggttcg ggctactctg
     3181 gccggggtac tagccggact ggcgattctg gctcccggca gtggcgcggt tggccgggcg
     3241 actccgcgca tcgacccgcc gaacgccacg gcctattcct attactacca ttacgcctgg
     3301 tacgaccacc ccgagccccc gtccgctgcg cccatgaacc ttggcgatcc cctcatcggc
     3361 cctggtatcc ctatggatca ggatctgcgc gccctggtgt aaggctggcg ggaattatcg
     3421 gcgcaagcaa acgcgtacac gcggccctaa accaggcaag gacccgtacc atggaacagt
     3481 tgggcaaact cagacggaaa gcaatcgtgg acctgggagt ggcggtgggt atggctctct
     3541 ctgccggcag cgtgggggcc gcgtatccca cgaatacgag cgccgtattc caatgccgag
     3601 taagatcgaa acctctgtag cagggcgcag cgccgtagtg gagtatggtc tcctatcagg
     3661 cttcctcagc gtgaatgaat caggagtggc acctctagcc cggcaggcac gagcccgtcc
     3721 tgggcatagg cgaccgcctg ctcgatggtc atcgcctggc ccgcgctgcg tgcagcggcg
     3781 tagacatcgt cacccagtaa ttcacgcgcg gtggcagcgg cccgctcgaa aatgccacgc
     3841 tccacgtatt ggaggtccgt gccgttgctg cctacat
//
LOCUS       DASUYC010000302         2731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1592382, whole genome shotgun sequence.
ACCESSION   DASUYC010000302 DASUYC010000000
VERSION     DASUYC010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1592382"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2547)
                     /locus_tag="VF276_04900"
     CDS             complement(<1..2547)
                     /locus_tag="VF276_04900"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6606236.1"
                     /translation="MAESTTPRASGGDPTTSLRPALQALLSNARLVTGARAAALAVPA
                     GPEGRLTLVSVEGDALTAGATLDPETVPGLRVLPLPPPAPDVPAPRVCLLVLRGLVSN
                     EPGSALASCLLALAAVVHAMQVSEAARRAAPAPKEAPAPPPAQVGFDIPRTPEIVLER
                     TLQRICRLLEADLASVAIRDRRGEIVWRGMAGYRSPIAPHTPVGPGAGLARRVTTTGQ
                     LQYLPEADPAIPLMIEEGVDSALGVPVCIDDQPVGALIIGWRRPRAVSGEEQRLVLAL
                     ADQVAGAVAQTQLYEETYRQRNFLQNLINHAPAGIVAFTLPDFRVREVNRFYLQFLEE
                     PFRSGSRPLQGLRLDDFLPQATENGVQAIFDRVAASGEPFTIREFEYKGFSRGTTYWN
                     WSLVPLQDTPDEAPTGLLLLAVESTEQVLARRRLAAALARARDQAGELEAVIQQMVEG
                     VAICDAEGNLTKINPAGVAMLGRGVIAREPSAAYPERYQLYDMEGNLFPAANLPTERA
                     RRGETVLGQDMVVRAPDGRERIISVSAAPLMDEANNITGGVAVFHDVTKAREVERLKD
                     DFLAIVSHELRTPLAAILGYTDIMLRGLGGPLTPKQEQNQQSIKANATRLLALINDLL
                     DVSKLEAAGVTLSAEAVSLQQVIPKAVAWVQVLASGRGVTVLHQVPANLPPVWGDAER
                     LHQVLTNLLSNAIKFTPAGGTVVVQARNSILPADAPPDSPDSAFAPPVGESILVSVQD
                     TGVGLAGDDLARIWDRFYQADSTTSRRYGGTGLGLTIVKSLVELHGGRVWATSAGTGK
                     GSTFQFRLPLAAHAAPVDQPEETGPDAAGAPAAEATATPAPPLILVIEDNI"
BASE COUNT          404 a          957 c          929 g          441 t
ORIGIN      
        1 gatgttgtct tcgataacca ggatcagcgg cggcgccggg gtggccgtag cctccgccgc
       61 cggcgccccg gcagcatccg gccccgtctc ctccggctga tcgaccggcg ccgcgtgggc
      121 cgccaggggc agccggaact ggaacgtgct gcccttgccg gtcccggcgc tggtggccca
      181 gacccggccc ccgtgcaact ccaccaggct cttgacgatg gtcagcccca ggccggtccc
      241 gccgtagcgc cggctcgtgg tcgagtcggc ctggtaaaag cgatcccaga tgcgggccag
      301 gtcgtcgccg gccaggccca cgccggtgtc ctgcacgctg accaggatgc tctcgcccac
      361 cggcggcgca aacgccgaat cggggctatc gggcggggca tccgcaggca agatgctgtt
      421 gcgcgcctgc accaccacgg tgccgcccgc cggggtgaac ttgatcgcgt tggacaataa
      481 gttggtgagc acctggtgca gccgctcggc atcgccccag acaggcggca ggttggccgg
      541 cacctggtgc agcacggtca caccccgccc cgacgccagc acctgcaccc aggcgaccgc
      601 cttggggatc acttgctgca aggacaccgc ctcggcgctg agggtgacac ccgctgcctc
      661 cagcttcgag acatccagca ggtcgttgat cagggccagc aagcgtgtcg cgttggcctt
      721 gatcgactgc tggttttgct cctgcttggg ggtaagcggc ccgcccagcc cgcgcaacat
      781 gatatcggta tagcccagga tggcggccag cggcgtgcgt agttcgtggc tgacgatggc
      841 gaggaagtcg tccttcaggc gctcgacctc gcgcgccttg gtcacgtcgt ggaagaccgc
      901 gaccccgccg gtgatgttgt tggcctcatc catgagcggg gcggcgctca cgctgataat
      961 ccgttcccgc ccatcggggg cgcgcaccac catatcctgg cccagcaccg tctcgccccg
     1021 gcgggcgcgc tcggtgggca gattcgccgc ggggaagagg ttgccttcca tgtcgtacaa
     1081 ctggtagcgt tcggggtacg cggcgctcgg ctcgcgggcg atcacgcccc ggcccagcat
     1141 ggcgactccc gccgggttga tcttcgtgag attgccctcg gcgtcgcaga tcgccacgcc
     1201 ctcgaccatc tgctggatca ccgcttcgag ttcgccggcc tggtcgcgtg cccgcgccag
     1261 ggccgcggcg agccgccgcc gcgccagcac ctgctcggtg ctctccaccg ccagcagcag
     1321 caagcccgtg ggcgcctcgt ccggcgtgtc ttgcagcggc accaggctcc agttccaata
     1381 ggtggtgccg cgactgaagc ctttgtactc aaactcgcgg atcgtgaacg gctcaccgct
     1441 ggccgccacc cggtcgaaga tggcctgcac cccgttctcg gtggcttgcg gcagaaagtc
     1501 gtcgagccgc aagccttgca gcgggcggct gccgctgcgg aaaggctcct ccaggaattg
     1561 tagatagaag cggttgacct cgcgcacgcg gaaatcgggc agggtgaacg ccacaatgcc
     1621 cgccggggcg tggttgatca ggttttgcag gaaattacgc tggcggtagg tctcttcata
     1681 aagctgcgtt tgtgcgaccg cgccggctac ctgatcggcg agggccagaa ccaggcgctg
     1741 ctcttcgccc gatacggccc gcgggcggcg ccagccgatg atcaacgcgc cgacgggctg
     1801 gtcgtcgatg caaaccggca cgcccagcgc gctgtccacc ccttcctcga tcatcaaggg
     1861 aatggccgga tcggcttcgg gcaaatattg caactgcccg gtcgtagtca cgcgccgggc
     1921 caggccggcc cccggcccca ccggcgtgtg gggtgcgatg gggctgcggt agcccgccat
     1981 gccccgccag acgatttcgc cgcgccggtc gcggatcgcc acactcgcca ggtcggcttc
     2041 gagcaggcgg cagatgcgct gcagggtgcg ctccagcacg atctccggcg tgcggggaat
     2101 atcgaacccc acctgcgctg gtggcggcgc cggtgcctct ttaggggcag gtgcggcgcg
     2161 ccgggccgcc tcgctcacct gcatggcatg gacgaccgcg gccagcgcga gcaggcacga
     2221 ggccagcgcc gagccgggtt cgttggacac caggccccgc agcaccagca agcacaccct
     2281 gggcgccgga acatcgggcg cgggcggcgg cagcggcagc acccgcaagc cgggcacggt
     2341 ctccgggtcc agggtggccc ccgccgtgag cgcgtcgccc tccacgctga cgagcgtgag
     2401 ccgaccctcc ggccccgcgg gcacggccag cgcggcggcg cgcgcccctg tcaccaggcg
     2461 tgcgttgctc agcagcgcct gcaaggcagg ccgcagactc gtcgttggat ccccgccgct
     2521 cgcgcgcggg gtggtggatt cagccatatc cgctgctagt gttctctgta gttgattaga
     2581 gagtttggtc actctgagcg cggcgaagag tctcgtcgtt tgaccagtag gacggcgatt
     2641 cttcgctccg ctcaggatgg tagttccccg ctggcagggt tctccgatct cggtttatgt
     2701 aaacaacgcc caggtagtcc cgatagtgca a
//
LOCUS       DASUYC010000303         6438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_32066,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000303 DASUYC010000000
VERSION     DASUYC010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6438
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32066"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VF276_04905"
     CDS             <1..663
                     /locus_tag="VF276_04905"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEX6606237.1"
                     /translation="EDAIPMPGATSYKQLRVNGLQWPCFAADQPDSPVLYAGGKLPRP
                     AQIAAAPALGGGAAPADGTLLMIAGFSLFPFRTGTQSRHSKSLAKVQPHSRLHLHPAD
                     AQRLGLRDVEQVRVSAATAPRNGHGSGDLSELNTRPVDAITLVSDAVPQGTAFLGLTM
                     AQAGRSPLARQALAAQTGGAKAVAIRVEPLYGVPTGDATIGPGPATGNVVDRANATLP
                     PA"
     gene            697..1983
                     /gene="nuoH"
                     /locus_tag="VF276_04910"
     CDS             697..1983
                     /gene="nuoH"
                     /locus_tag="VF276_04910"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX6606238.1"
                     /translation="MDFDWVWLLIRFIVAVIILTFVLVTMGFLTYFERKVAAHMQHRM
                     GPNRTGPIGLLQWVADAVKLISKEEIIPAGADKVVFWLAPMLSMFTASAAYAFIPFGE
                     QITLFDHSIPYYISETPIGLIALLAFSSLGVYGLIMGAWSSNNKYSLLGGLRSSAQVI
                     SYEVTMGISLIGPMILAQSLSLQDFARAQGGSLIGAGSPLGTPGVWFILLQPVAFMTY
                     LTSAIAETNRIPFDLPESESELVGGYHVEFSAMHFGLYFLAEYINMITVSAIATYCFL
                     GGWQPIIPIGGAALGPIWFILKMSFFIFFYYWLRWTLPRFRYDQLMGLCWKAILPLVL
                     VNILVVAIMRALLPPDIPPLFHGSLEQTWPWLVLIVIQLAFAAAVIFAASRAAVGTWW
                     GKSEKPVFVRDAGPAPLAAALDTTEGQRRIKLRQGL"
     gene            1995..2645
                     /locus_tag="VF276_04915"
     CDS             1995..2645
                     /locus_tag="VF276_04915"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HEX6606239.1"
                     /translation="MELTDQVTGFLLTLRTMFEKTVTVQYPDALRELAPRFRGAVGFL
                     PHPSTGREKCVGCGLCPQICPVDAITIGIIEDETENADHTDEWHPFEHYYMKGLDPLD
                     PDSKKFSYYYHINETRCLYCGLCVEVCPVEALVMSHHFEMSTDNRGETIYDKHKILQM
                     GKTYMAELNERLEGVQVRPQLPGQTRRPTQLGRPAGVEQPGVGAEAYGNEEKTAQK"
     gene            2686..3522
                     /locus_tag="VF276_04920"
     CDS             2686..3522
                     /locus_tag="VF276_04920"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX6606240.1"
                     /translation="MTIAIPSLPQIVFLGLAVIAVAAALLMITRNDAVHSALALVIVL
                     FQLAGMYVLLNAPFLAVLQVLVYAGAILVLFLFVIMLLQLRSGPQLGKEHTIQRVTAW
                     PIGLLLGAELVLVIVLAASLQAPPIPAGTNLPNPGGGWPVTRIDTYGGEPRALGIELY
                     TNFLLPFEVASLILLVAVLGAIVLARREEGALTEIVTRGISLGRIAPLGSAQAAEIEK
                     TLAGEVPNVRVMSGALAGDTPAPPVPVAAGSQPDVTDRRSPPSSSTDVSEDRPQNNGP
                     TA"
     gene            3555..3878
                     /gene="nuoK"
                     /locus_tag="VF276_04925"
     CDS             3555..3878
                     /gene="nuoK"
                     /locus_tag="VF276_04925"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX6606241.1"
                     /translation="MPTDMGAPVPLSWFLILSGVLFAIGMIGVLTRRNVLVIFMSVEL
                     MMNAVNITLVAFGTAMNSLAGQAFVVFVVTVAAAEAAVGLAIIMALVRHKPTVDAAEM
                     EIMKE"
     gene            4020..6239
                     /gene="nuoL"
                     /locus_tag="VF276_04930"
     CDS             4020..6239
                     /gene="nuoL"
                     /locus_tag="VF276_04930"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX6606242.1"
                     /translation="MPSVLNLIWLIPALPLLGMLVTGLGGRALTNGGQRLLASYIATA
                     CVLVSLLISIVVAFNVADRNGATYNSTLYTWIPSGDFVVDIGFWVDGLTAVMLLVVTG
                     IASLVHIYAIGYMRADARDPSVQAHHGAHDTHAAPAAHGAADAHASHEAHGEHGAGES
                     GSVPESGTPPINPADKPGDGGWRIRGGLQKDGGLARFFAFLNLFVFSMLVLVLANNYL
                     MLFVGWELVGLSSYLLIGFWYQKRDWFQDRKGFIRKDVPFEASKKAFIVNRIGDFGFT
                     IGIITLFINFGSLSFGNIFPNAAAQANHFVLGNMTITCLLLFMGAMGKSAQFPLHVWL
                     PDAMAGPTPVSALIHAATMVTAGVYMVARSNPLFHLAPDAMLVVLVIGTVTALIGATI
                     ACTATDLKQVVAYSTVSQLGYMFAGLGAGVWIAAIFHLFTHAFFKGLLFLGSGSVLHG
                     MHDLESEGKDGQDIRWMGGLFPYMKVTAITFIIGALANAGIVPFAGFWSKDEIISGAI
                     LNGFPIAGGLLWIGSFLTAFYMFRLVFLVFFNRRRWDPAVVHPHESPPVMTVPLLILG
                     GAATVVGFLGFPPEAGLFHQYLAPVFSGVMHEAGSGYAPAHIGQTLTLQFISVLFALA
                     GIGLAFMLYWRPSSAAETMSGWIPSLWRALRMRWYFDELYDLVFVRGGKAVANGLWAF
                     DRNVVDGAVNGVAALFRWGGARLRRTQTGFVANYAYAIALGVFVLLGYVLVSTLVAK"
BASE COUNT          979 a         2085 c         2130 g         1244 t
ORIGIN      
        1 gaggacgcca tcccgatgcc gggcgccacg tcgtacaagc agttgcgcgt gaacggcctg
       61 caatggccgt gcttcgcggc cgaccagccc gacagcccgg tgctctatgc cggcggcaag
      121 ctgccgcgcc cggcccagat cgcggcggcg cccgcgctgg gcggtggggc cgccccggcg
      181 gacggcacgc tgctgatgat cgccgggttc agcctgttcc cgttccgcac cggcacccag
      241 agccgccaca gcaagtcgct ggcgaaggta caaccgcata gccggctgca tctgcacccg
      301 gcggacgccc agcggctggg gctgcgcgat gtcgagcagg tgcgggtcag cgccgccacc
      361 gcgccgcgca acggccacgg ctccggcgat ctctccgagc tgaacacccg gcccgtggac
      421 gccattaccc tggtcagcga tgcggttccg cagggcaccg ccttcctggg gctgacgatg
      481 gcccaggccg ggcgcagccc gctggcccgc caggccctgg ccgcgcaaac cggcggcgcg
      541 aaagccgtcg ctatccgcgt tgagccgctc tatggcgtgc ccaccggcga cgccactata
      601 ggtcccggcc cggccaccgg caacgtcgtc gaccgggcca acgcgacctt accgcccgcc
      661 tagcgctgct ggatcatccc tggagggagg ggaacgatgg attttgattg ggtatggttg
      721 ctgatccggt ttatcgtcgc cgtgatcatc ctgaccttcg tgctggtcac gatgggcttc
      781 ctgacctact ttgagcgcaa ggtggcggcc catatgcagc accgcatggg gcccaaccgc
      841 accgggccta tcggcttgct gcaatgggtg gccgacgcgg tcaagctgat cagcaaggaa
      901 gagattatcc cggcgggggc cgacaaggtg gtcttctggt tggcgccgat gttgagcatg
      961 ttcacggcgt cggcggccta tgcctttatt ccgttcggcg agcagatcac gctcttcgac
     1021 cactcgatcc cgtactacat ctctgagacg ccgatcgggc tgatcgccct gctggccttc
     1081 tcgtcgctgg gcgtgtacgg gttgatcatg ggtgcgtgga gttccaacaa caaatactcc
     1141 ttgctgggcg gtttgcgctc cagcgcgcag gtgatcagct atgaagtgac catgggcatc
     1201 tcgctcatcg gcccgatgat cctggcccag tcgctgagcc tgcaagactt cgcgcgggcg
     1261 cagggcggat cgctgatcgg cgccgggtcg ccactgggga cgccgggcgt ctggtttatc
     1321 ctgctgcagc cggtcgcctt catgacctat ctcacctcgg ccatcgccga gaccaaccgc
     1381 ataccattcg atctgcccga atccgaatcc gaactggtgg gtggctacca cgtcgagttc
     1441 agcgccatgc acttcggcct ctacttcctg gccgaataca tcaacatgat caccgtgtcg
     1501 gccatcgcca cctactgttt cctgggcggc tggcagccga tcattcccat cggcggcgcg
     1561 gcgctcggcc cgatctggtt tatcctgaag atgagtttct tcatcttctt ctattactgg
     1621 ctgcgctgga cgctgccgcg cttccgctac gaccagctga tgggcctgtg ctggaaggcg
     1681 attctgccgc tggtgctggt caatatcctg gttgtggcga tcatgcgcgc gctgctgccg
     1741 cccgacatcc cgccgctgtt ccacgggtcg ctggagcaga cgtggccgtg gctggtgctt
     1801 atcgtcatcc aactggcgtt cgccgcagcg gtaatcttcg ctgcctcgcg ggccgcggtg
     1861 ggcacctggt ggggcaaatc cgagaagccg gtgtttgttc gcgacgcggg accggccccg
     1921 ctggccgcag cgctcgacac cacggagggg cagcgccgca tcaagttgcg ccaggggctg
     1981 tagggagagg ctaaatggaa ctgacggatc aggttacagg gttcttgctg accctgcgca
     2041 cgatgttcga gaagacagtc actgtgcagt accccgacgc gctccgcgag ctggcgccgc
     2101 gcttccgtgg cgcggtcggc ttcttgccgc acccgtccac ggggcgcgag aagtgcgtcg
     2161 gttgcggcct ctgcccgcag atctgcccgg tggacgccat caccatcggc atcatcgagg
     2221 acgagaccga gaacgccgat catacggacg agtggcaccc cttcgagcac tattacatga
     2281 aggggctgga cccgctcgac cccgacagca agaagttcag ctactactat cacatcaacg
     2341 agacgcgctg cctctactgc ggcctgtgcg tggaagtctg cccggtcgaa gcgctggtga
     2401 tgagccacca cttcgagatg tctaccgaca accgcggcga gacgatctac gacaagcaca
     2461 agattttgca gatgggcaag acctacatgg ccgaactgaa cgagcgactg gaaggcgtgc
     2521 aggtgcgccc gcagttgccc ggccagacac ggcgcccgac gcagttgggc cgcccggcgg
     2581 gggtcgagca gcccggtgtg ggcgccgagg cctacggcaa cgaagagaag acggcccaga
     2641 aatagccagg ggtgcgcttg ccccttgcag tgtggagcat agaagatgac tattgcaatc
     2701 ccgtcgctac cgcagatcgt gtttctcggc ctggccgtga ttgccgtcgc ggcggcgctc
     2761 ttgatgatca cgcgcaacga cgccgtgcat agcgcgctgg cgctggtcat cgtgttgttc
     2821 caactggccg ggatgtacgt cctgctgaac gcgccgttcc tggccgtact ccaggtgctg
     2881 gtctacgccg gggccattct ggtgctcttc ctgttcgtga tcatgctgct gcaactgcga
     2941 tccggcccgc agttaggcaa ggaacacacc atccagcgag tgacggcctg gccgattggg
     3001 ctgctgctgg gcgccgaact ggtgctggtg attgtgctgg ccgcgtcgtt gcaggcgccg
     3061 ccgatccccg ccggcaccaa cctgcccaat ccgggcggcg gctggccggt cacgcgcatc
     3121 gacacctacg gcggcgagcc gcgcgcgctg ggcatcgagt tgtataccaa tttcctgctg
     3181 ccgtttgaag tggcgtcgct gatcctgctg gtcgcggtgc tgggggccat tgtgctggcc
     3241 cgccgggaag agggggcgct caccgagatc gtcacgcgcg gcatcagcct ggggcgcatc
     3301 gccccgctgg gatcggcgca ggcggcggag atcgagaaga ccctggccgg cgaggtgccc
     3361 aacgtgcggg tgatgtcggg tgcgctggcc ggcgatacgc ccgcgccgcc ggtgccggtg
     3421 gccgcgggga gccagcccga cgtgaccgac cggcgctcgc cgcccagttc gtccacggat
     3481 gtcagcgaag atcgcccgca gaataacggc ccgacggcct agacgtttgc gaatgcgtag
     3541 cctggagaga gtgtatgcct actgatatgg gcgccccggt gccgttgtcg tggttcctga
     3601 tcctgagcgg tgtcctattc gccatcggca tgatcggcgt gctgacccgg cgcaacgtgc
     3661 tggtcatctt tatgtcggtt gaactgatga tgaacgccgt gaatatcacg ctggtcgcct
     3721 tcggcacggc gatgaactcg ctggccgggc aggcgttcgt ggtgtttgtg gtcacggtgg
     3781 cggcggcgga ggcggccgtg ggcctggcga ttattatggc cctggtgcgc cacaagccga
     3841 ccgtcgacgc cgccgagatg gaaatcatga aggagtgacc cgcggcggcg ggccagggcc
     3901 gggcggctgc gattgtggcg cccgccccgg ccggcagtag tggagtccgt cttaccggcc
     3961 cacgccaggg ccgatcatgc tcaaccgata actcgtggct tcccggaggg aattggtcca
     4021 tgccttctgt gctcaacctg atctggctga tcccggcgtt accgctgctc ggcatgctgg
     4081 ttacgggtct cggcgggcgc gcgctcacca acggcgggca gcggctgctg gcttcgtata
     4141 tcgccacggc ctgtgtgctg gtctcgctgc tgatctcgat cgtcgtcgct tttaacgtgg
     4201 ccgacaggaa cggcgccacc tataactcga cgctctacac ctggatccct tccggtgact
     4261 tcgtggtcga tatcggcttc tgggtggacg gcctgaccgc ggtgatgctg ctggtcgtga
     4321 ccggcatcgc ctcgctggtg catatctacg ccatcggcta tatgcgcgca gatgcccgcg
     4381 atccgtcggt gcaggcgcac cacggcgccc acgacaccca tgccgccccg gcggcccacg
     4441 gcgccgccga tgcccatgcg agccatgaag cccacggcga acacggcgcg ggcgagtcgg
     4501 gcagcgtgcc cgagtccggg acgccgccga tcaacccggc ggataagccc ggcgacggcg
     4561 gctggcgcat ccgcggtggg ttgcagaagg acggcggcct ggcccgcttc ttcgccttcc
     4621 tcaacttatt cgtcttcagt atgctggtgc tggtgttggc aaacaactac ctgatgctgt
     4681 tcgtcggctg ggaactggtg gggctctcgt cctacctgct gatcgggttc tggtatcaga
     4741 agcgggactg gttccaggac cgcaaggggt tcatccgcaa agatgtgccg ttcgaggcgt
     4801 cgaagaaggc gtttatcgtc aaccgcatcg gcgacttcgg tttcacgatc ggcatcatca
     4861 cgttgttcat taacttcggc agcctgtcgt ttggtaacat tttccccaac gcggccgcgc
     4921 aggcgaacca ctttgtgcta ggcaatatga ctatcacctg cctgctgctg ttcatgggcg
     4981 ccatgggtaa gtcggcgcag ttcccgctgc acgtctggct gcccgacgcc atggccggtc
     5041 ctaccccggt gagcgccctg atccacgcgg cgacgatggt cacggcgggc gtctacatgg
     5101 tggcccgctc gaacccgctc ttccacctgg cgcccgatgc catgctggtc gtgctggtca
     5161 tcggcacggt gacggccctg atcggggcga ccatcgcctg cacggccacc gacctcaagc
     5221 aggtcgtcgc ctattcgacg gtcagccagc ttggctacat gttcgcgggc ctgggcgcgg
     5281 gtgtctggat cgccgccatc ttccacctgt tcacgcacgc cttcttcaag ggcctgctct
     5341 tcctcggttc cggctcggta ttgcacggca tgcacgacct ggagagcgag ggcaaggacg
     5401 gccaggatat tcgctggatg ggcgggctgt tcccgtacat gaaggtcaca gcgattacgt
     5461 ttatcatcgg cgcactggcg aacgccggga tcgtgccgtt cgcgggcttc tggagcaagg
     5521 acgagatcat cagcggcgcc atcctcaacg gcttcccgat cgccgggggc ctgctctgga
     5581 tcggctcgtt cctgacggcg ttctacatgt tccgcctggt gttcctggtc ttcttcaacc
     5641 ggcgccgctg ggacccggca gtggtccacc cgcacgaatc cccgccggtg atgacggtgc
     5701 cgctgctgat cctgggcggg gcggccaccg tggtcgggtt cctgggattc ccgccggaag
     5761 cgggcctgtt ccaccagtac ctggcaccgg tgttcagcgg ggtgatgcac gaggcgggca
     5821 gcggctatgc gccagcgcac atcgggcaga cgctgactct gcaattcatt agcgtgctgt
     5881 tcgcgctggc cggcatcggg ctggccttca tgctctactg gcggcccagc agtgccgccg
     5941 agacgatgtc cggctggatc ccgtcgctgt ggcgggcgct gcgcatgcgc tggtacttcg
     6001 acgagctcta tgacctggtc tttgtgcgcg gcggcaaggc cgtggcgaac gggctgtggg
     6061 ccttcgaccg caacgtggtc gacggcgccg tcaacggcgt ggccgcgttg ttccggtggg
     6121 gcggcgcgcg cttgcgccgg acccagaccg gcttcgtcgc caactacgcc tacgccatcg
     6181 cgctgggtgt gtttgtcttg ctcggatacg tgttggtttc cacgctggtt gccaagtagc
     6241 cggcgttgag tgcgagggga tacccgtgaa cgactggatc ctgaccctga tcacgttcgt
     6301 gccaatcgtc ttcgtgatca tcatgttgtt accgatgtgg cagacgccgc agttcaagat
     6361 catcgccacg gtgggcgccg ccatcgatct ggtgctgtcg ctgctgctgc tgcccttcta
     6421 taactacgcg ccggggct
//
LOCUS       DASUYC010000304         2806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1503614, whole genome shotgun sequence.
ACCESSION   DASUYC010000304 DASUYC010000000
VERSION     DASUYC010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1503614"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(230..1432)
                     /locus_tag="VF276_04935"
     CDS             complement(230..1432)
                     /locus_tag="VF276_04935"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6606243.1"
                     /translation="MLKLLNARLIDGTGAGPQDGATVLLDGSRIAAVYGPGSTPPSND
                     DPVIDLHGRTLLPGLINAHIHILMDDNAGDSFGSITRLSLPALALRGVQHGRRILEAG
                     ITTARELGGYEYVELELRDAFARGDFPGPRLLCAGKIITMTGGHGWPLGRQADGADEV
                     RKAVREQLRAGADVIKLMATGGVLTPGVEPGAAQLTEDELRAGIEEAHRADRRTASHA
                     QGTTGVKNAIRAGIDSIEHGIYLDDEAVEMMRDRGVVLVPTLAAPYQIVVAGEARGIP
                     AYAVEKSRRVMEAHFDSFKRAVAAGVTIAAGNDGGTPFNPAHDLVTELRLMITHGMSA
                     VEALRTAGHGSAVALGLADEIGTVQAGKQADLLVVDGDPLADIAALGWPWLVLKGGQV
                     VYRAAGDH"
     gene            complement(1452..>2806)
                     /gene="recJ"
                     /locus_tag="VF276_04940"
     CDS             complement(1452..>2806)
                     /gene="recJ"
                     /locus_tag="VF276_04940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEX6606244.1"
                     /translation="NRAALDELAKQAATLVITVDCGISNVEEVTHGQGLGLDLIVTDH
                     HNAPNTLPNTILINPKLGTDGDAFTNLTGVGVAHQVVRALVERLGRPPSLRNNDLLEL
                     VAIGTVADIAELKGANRTLVALGVQCLRKTRRPGLLALLAAASLKPELISTHTIGFII
                     APRINAAGRLDDAKMAYQLLLTEDPAEAADLAAKLEAENARRQELTRAIQDEARKRVA
                     DLDQDTPVIILRDKSWDMGVVGLAAGRLCEEFGRPAIVFADDGEMCRGSARSTTHFNI
                     HDVLEQVEDLLTHFGGHTAAAGMSMPSANFDEFCQRVTELVESELSDESQLAGVLQAD
                     YELPLSRVNVETMAELAALEPYGPGNSRPMFVARNLRVLDHKTNGKDGRTLTMNLAPG
                     DDPGARPVRAVAFGQAPEWAPKLADKPKIDIMFRLEADEWQGQQRVQLRVEGLRLSKA
                     "
BASE COUNT          408 a          936 c          969 g          493 t
ORIGIN      
        1 cgtcattctg agcgcagcga agaatctccg gtcggtaagc ccacaagacg agactcttcg
       61 ctgcgctcag agtgacaatt ccgtacacct tcaaccaagg tccgtcattc tgagcgcagc
      121 gaagaatctc cggtcggtaa cgcaaaagac gagactcttc gctgcgctca gagtgacaat
      181 tcacaaagga cgagactctt cgctgcgctc agagtgacaa ttcacaaagt tagtggtccc
      241 cggcggcgcg gtagaccacc tggccgccct tgagcaccag ccagggccag cccagcgcgg
      301 cgatgtcggc cagcgggtcg ccgtccacaa ccagcagatc ggcctgcttg cccgcctgca
      361 cggtgccaat ctcatcggcc agccccagcg cgacagccga gccgtggccc gccgtgcgca
      421 gcgcctcgac ggcgctcatg ccgtgggtga tcatcaggcg cagttcggtc acgaggtcgt
      481 gggccgggtt gaacggggtg ccgccgtcgt tgcccgcggc aatggtcact ccggcggcca
      541 cggcgcgctt gaaggagtcg aaatgggcct ccatcacccg gcgcgacttc tcgacggcat
      601 aggccgggat gccgcgcgcc tccccggcga cgacgatctg gtagggcgcg gccagcgtgg
      661 gcaccagcac cacgccccgg tcgcgcatca tctccaccgc ttcgtcgtcc aggtagatgc
      721 cgtgctcgat gctgtcgatg cccgcccgga tggcgttctt gacgccggtg gtgccctggg
      781 catgggaggc ggtgcggcgg tcggcgcggt gcgcctcctc gatcccggcg cgcaattcat
      841 cctcggtcag ttgggcggcg cccggctcca cgcccggcgt cagcacgccg cccgtcgcca
      901 tcagcttgat cacgtcggcg cccgcgcgca gttgctcgcg caccgccttg cgcacctcgt
      961 cggccccgtc ggcctggcgt cctagcggcc agccgtgccc gccggtcatg gtgatgattt
     1021 tgcccgcgca gagcaggcgg gggccgggga agtcgccgcg ggcaaaagcg tcgcgtaatt
     1081 ctagctcgac atactcgtag ccgcccagtt cgcgagcggt ggtgatgccg gcctccagga
     1141 tgcggcggcc atgttgcacg ccccgcaagg cgagcgccgg gagcgacagg cgcgtgatgg
     1201 acccgaagct gtcgccggcg ttgtcgtcca tcaggatgtg gatgtgggcg ttgatcaggc
     1261 cgggcaggag ggtgcgcccg tggaggtcga tgaccgggtc gtcgttgctg ggcggggtgc
     1321 tgccggggcc gtagacggcg gcgatgcggc tgccgtcgag gagcacggtg gccccgtcct
     1381 gcggcccggc gccggtgccg tcgatcagcc gcgcgttcag cagtttcagc atggcccctc
     1441 ccgcgtaccc tttacgcttt gctcagccgc aacccttcca cgcgcagttg gacgcgctgc
     1501 tggccctgcc actcgtccgc ttccaggcgg aacatgatgt cgatcttcgg cttatcggcc
     1561 agcttcggcg cccactccgg ggcctggccg aaggcgacgg cgcgcaccgg gcgggcaccc
     1621 ggatcgtcgc ccggcgccag gttcatggtt agcgtgcgcc cgtccttgcc gttggtcttg
     1681 tggtcgagga cccgcaggtt acgtgccaca aacatgggcc gcgaattacc ggggccatac
     1741 ggctccagcg cggccagttc ggccatagtc tcgacgttga cccggctcaa cggcagttcg
     1801 tagtcggcct gcaacacccc ggccagttgc gactcgtcgc tcagttcgct ctcgaccagt
     1861 tcggtcaccc gctggcaaaa ctcgtcgaag ttggcgctgg gcatgctcat gccggcagcg
     1921 gctgtatggc cgccgaagtg cgtgagcagg tcttcgacct gctccaatac gtcgtggatg
     1981 ttgaaatgcg tcgtggagcg ggcgctgccc cggcacatct cgccgtcgtc ggcgaacacg
     2041 atggcggggc ggccaaactc ctcgcacagc cgcccggcgg ccaggcccac cacacccatg
     2101 tcccagcttt tgtcgcgcag gatgatcacc ggcgtatcct ggtcgagatc ggccacgcgc
     2161 ttgcgcgctt cgtcctggat ggcgcgggtc agttcctggc ggcgggcgtt ctcggcttcg
     2221 agtttggcgg ccaggtcggc cgcctcggcg gggtcctcgg tcagcagcaa ctggtaggcc
     2281 attttggcgt cgtcgagccg cccggcagcg ttgatgcgcg gggcgatgat gaagccgatg
     2341 gtgtgcgtgg aaatgagttc gggtttgagc gaggcggcgg ccagcagcgc cagcaggccg
     2401 ggccgccggg ttttgcgcag gcactgcacg ccgagcgcga ccagggtgcg gttcgcgccc
     2461 ttcagttcgg cgatgtcggc gaccgtgccg atggcgacca gttcgagcag atcgttgttg
     2521 cgcaggctgg gcgggcggcc caggcgctcg accagcgccc gcaccacctg gtgggccacg
     2581 cccacgccgg tcaggttggt gaaggcatcg ccgtccgtgc ccagcttggg gttgatcagg
     2641 atggtattgg gcaaggtgtt cggtgcattg tggtgatcgg tgacgatcag atcgaggccc
     2701 aggccctggc cgtgggtgac ttcctcgacg ttgctgattc cacagtccac ggtgatcacc
     2761 agcgtcgcag cctgtttagc gagttcatcc agggcggccc ggttca
//
LOCUS       DASUYC010000305         3473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_101150,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000305 DASUYC010000000
VERSION     DASUYC010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101150"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VF276_04945"
     CDS             complement(<1..515)
                     /locus_tag="VF276_04945"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453916.1"
                     /note="Catalyzes first step of the de novo purine
                     nucleotide biosynthetic pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX6606245.1"
                     /translation="MPEMPNFDLAEDTPLADDRLHEACGVFGVYAPHEDVARITYFGL
                     YALQHRGQESAGIATTTGRDLAVFRRMGLVAQVFDEAALTGLRGAESVAAIGHTRYST
                     TGSNRTCNAQPIEVHDPALGPLALAHNGNIINAPALKARLQEHGVHFAGTTDSEVIAE
                     ALAYTAGADWVA"
     gene            complement(549..2831)
                     /gene="purL"
                     /locus_tag="VF276_04950"
     CDS             complement(549..2831)
                     /gene="purL"
                     /locus_tag="VF276_04950"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF002290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEX6606246.1"
                     /translation="MSAEPIPTIEAGAAPAVPLAEVGLSPDEYAHLCSLLGRTPNAVE
                     LGMVGAMWSEHCGYKNSRPLLKLFPTHGPRVLQGPGENAGAVDIGDGLAVVFKIESHN
                     HPSAVEPVEGAATGVGGIVRDIFTMGARPIALLNSLRFGPLDEPRTRYLFGGVVAGIG
                     RYGNAIGVPTVGGEIYFHPSYSGNPLVNALCAGIIPSADLMRARAAGPGNPVLLVGAD
                     TGRDGIHGATFASDTLDAENEAKRPAVQVGNPFLEKCLIEACLELLQTGIVVGMQDLG
                     AAGLTSSVVEAASRGGVGIRIDVAQVPRRERGMNAYEIMLSESQERMLVVVQRGAEAQ
                     ADTIFARWGLHSARIGEVTTSGRVEIFDGPRQVADLPAPLLTDACPVYHRRGAPAPDL
                     AALQHPDWAALLPPELPGADYSAAWRTLLAAPNIASKAAVYRTYDHTIGTNTVLAPGQ
                     ADAAVLRVKGTPKGIALTTDCNARFCLLDPYQGGMQAVAEAARNVVCTGAEPLAVTDC
                     LNFGSPERAEIFYQMEGCVRGIVAACEALGTPVVSGNVSLYNETAGQAVLPTPVIGML
                     GLLDDVTQRVGMAWPADAQLALLAPPLAPLPDGGVAALGASEYLATIHGREAGAPPPI
                     DLDAERRVQAVALAAIRGGLVQAAHDCSEGGLAVAVAEGCIAGQVGADLDLGALGADL
                     RGDVLLFAEQPSRILLACPPGSRDALADLARQAGVVLVWLGATGGDTIRLRRGAQPIA
                     SLPLAGATDAWEHGLARAGA"
     gene            3037..>3473
                     /locus_tag="VF276_04955"
     CDS             3037..>3473
                     /locus_tag="VF276_04955"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX6606247.1"
                     /translation="MGHVDEIDRKILELLQDDARTPLAAIAKEVSLSGAAVAERVRKL
                     EQTGVIRGYQAIVDHEKLGKPLMAFVRVTQTGPGYDDDALQRSALPADPDIMEIHHVA
                     GEDCFILKVRAADPRGIEALLKRLRRRLHVDRTITMIVLSTVK"
BASE COUNT          546 a         1241 c         1176 g          510 t
ORIGIN      
        1 gccacccagt ctgcgccggc cgtgtaggcc agcgcttcgg cgatcacctc gctgtccgtg
       61 gtgcccgcaa agtggacgcc gtgctcctgc aagcgcgcct tgagggccgg agcgttgatg
      121 atgttgccgt tgtgggccag ggccagcggc cccagggcgg ggtcgtggac ctcgatgggc
      181 tgggcgttgc aggtgcggtt agacccggtg gtcgaatagc gagtgtggcc gatggccgcg
      241 acggactccg cgccgcgcag gccggtcagg gcggcttcgt cgaacacctg cgccaccagg
      301 cccatacgcc ggaacacagc caggtcgcgc ccggtcgtcg tggcgatgcc cgcgctctcc
      361 tgcccgcgat gctgcaaggc gtagaggccg aaataggtga tgcgcgcgac atcctcgtgc
      421 ggcgcgtaga ccccgaacac gccgcacgcc tcgtgcagcc ggtcatccgc cagaggggta
      481 tcctcggcaa gatcgaaatt cggcatttcg ggcatcaact cctccatcgc gcgaaccgcc
      541 cggcggggct acgcgccggc ccgcgccagg ccatgctccc aagcatcggt ggcgccggcc
      601 agcggcagac tcgcgatagg ctgcgcgccc cggcgcaggc ggatcgtgtc gccgccggtt
      661 gcgcccagcc agaccagcac caccccggcc tgccgcgcca gatcggccag cgcatcccgg
      721 ctgccgggcg ggcaggccag caggatgcgc gagggctgtt cggcaaagag cagcacatcg
      781 ccgcgcagat cggcgcccag cgcgcccagg tccaggtcgg cgcccacctg cccggcgatg
      841 cagccctcgg ccacggcgac agccagcccg ccttcgctgc aatcatgcgc cgcctggacc
      901 agcccgcccc ggatcgcggc cagggcgacc gcttgcacgc gccgctcggc atcgaggtcg
      961 atgggcgggg gcgcccccgc ttcccggcca tggatcgtcg ctaggtactc gctcgcgccc
     1021 agcgcggcca ccccgccatc gggcagcggg gccaggggcg gcgccagcaa ggccaactgc
     1081 gcgtccgcgg gccaggccat gcccacccgc tgcgtcacat cgtccagcag gcccagcatg
     1141 ccgatcaccg gcgtgggtaa cacggcctgc cccgccgtct cgttgtagag gctgacgttg
     1201 ccgctgacca ccggcgtacc cagcgcctcg caggcggcca cgataccgcg cacgcagccc
     1261 tccatctggt agaagatctc tgcgcgctcc gggctgccga agttaaggca atcggttacg
     1321 gccagcggct cggcgcccgt gcaaaccacg ttgcgcgccg cctcggccac ggcctgcatc
     1381 ccgccctggt aggggtcgag caggcagaag cgagcgttac aatcggtagt cagggcgatg
     1441 cccttgggcg tgcccttgac gcgcagcacg gcggcgtcgg cctggccggg cgccagcacc
     1501 gtgttcgtgc cgatggtatg gtcgtaggtg cggtagacgg cggctttgct cgcgatattc
     1561 ggggcggcca gcagcgtgcg ccaggcggcg ctgtaatcgg cgccgggcag ttcgggcggc
     1621 agcagcgccg cccagtcggg atgttgcagg gccgccagat ccggcgcggg cgcgccccgg
     1681 cgatggtaca ccgggcaggc gtcggtcagc agcggggcgg gcagatcggc cacctgccgc
     1741 gggccgtcga agatctccac ccgcccgctg gtcgtcacct cgccgatccg cgccgagtgc
     1801 aaaccccagc gagcgaagat ggtgtccgcc tgtgcttccg cgccgcgctg caccaccacc
     1861 agcatccgct cctggctctc gctgagcatg atctcgtagg cgttcatgcc ccgttcgcgc
     1921 cgcggcacct gggccacgtc gatgcggatg cccactccgc cccggctcgc tgcctccacc
     1981 accgacgagg tcagccccgc cgcgcccagg tcctgcatgc cgacgacgat gccggtttgc
     2041 aacagttcca ggcaggcttc gatgaggcac ttttcgagga aggggttgcc cacctgcact
     2101 gccgggcgct tggcctcgtt ctccgcgtcg agcgtgtcgc tggcgaaggt ggcgccgtgg
     2161 atgccgtcgc ggcccgtatc ggcgcccacc agcaacaccg ggttgccggg gccggcggcg
     2221 cgcgcccgca tcaggtcggc gctggggatg atcccggcgc agagcgcgtt gaccagcggg
     2281 ttgccgctat atgaggggtg aaaatagatc tcgccgccca ccgtgggcac gccgatggcg
     2341 ttgccgtagc gcccgatccc ggccaccacg ccgccgaaca ggtagcgcgt gcgcggctcg
     2401 tccagcgggc cgaagcgcag cgagttcagc agcgcgatgg gtcgcgcgcc catggtgaag
     2461 atgtcacgca cgatgccgcc gacgcccgtg gccgcgccct cgaccggctc cacggcgctg
     2521 ggatggttgt ggctctcgat cttgaagacc accgccagcc cgtcgccgat atcgacggcg
     2581 cccgcgttct cgcctgggcc ttgcagcacg cgcggcccat gggtggggaa aagcttcagc
     2641 agggggcggc tgttcttgta gccgcagtgt tcgctccaca tcgcgcccac catgcccagt
     2701 tccacggcat tgggggtgcg accgagcaaa gaacagaggt gcgcgtactc gtcgggcgac
     2761 aaacctactt cggccagggg caccgcgggc gcggcaccgg cctcgatggt tggtattggt
     2821 tcagcggaca tcagactcct tgtcctggac ctctggcagc caatcgcttc ccctcattat
     2881 accacaccgc caatcccgcc accgccgccc cggcgcgtcc gcccgccgcc ttcgatccgg
     2941 agactcgccc aagatccgcc tttcgattga taggcaaaac gggggaaaca ctatataatt
     3001 gcaagtattg tgccggtttt accaaaggaa cgcaagatgg gtcatgtaga cgagatcgac
     3061 cgcaagatcc tggaactgct ccaggacgac gcccgcacgc ccctggcggc catcgccaaa
     3121 gaagtcagcc tcagcggggc ggcggtggcc gagcgggtgc gtaaactgga acagaccggc
     3181 gtgatccgcg gctaccaggc cattgtggac cacgaaaaac tgggcaagcc gctgatggcc
     3241 ttcgtgcgcg tcacccagac aggaccgggc tacgacgacg acgccctgca gcggtcggcc
     3301 ttgccggcgg accccgacat aatggagatc caccacgtgg ccggcgagga ctgttttatt
     3361 ctcaaggtgc gcgccgccga tccgcggggc atcgaagccc tgctcaagcg cctgcgcagg
     3421 cggctgcatg tagaccgcac aatcacgatg atcgtcttgt ccacggtaaa aga
//
LOCUS       DASUYC010000306         2356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1187850, whole genome shotgun sequence.
ACCESSION   DASUYC010000306 DASUYC010000000
VERSION     DASUYC010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187850"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VF276_04960"
     CDS             <1..225
                     /locus_tag="VF276_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606248.1"
                     /translation="VCVDRAVAASGLQPEQIDVVLRTGGSSSIPAFVRLLERRFGPAK
                     IRKQDVFTGIAAGLGIAGYRSLLPPVARTA"
     gene            complement(267..1799)
                     /locus_tag="VF276_04965"
     CDS             complement(267..1799)
                     /locus_tag="VF276_04965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX6606249.1"
                     /translation="MSQQVINTAPRPAAPATAVPWRLTPGAVALAVVVLGGVLRLYRY
                     TALSLWVDEGITIGVSRLPWPDVLGLHGAYEVHPPLYFALTKAMSLLAPETDAGRLVS
                     VIAGTLTLAVVYRLGARLVAPWAGVVAALGLAVAPLHVWYSQEGRPYALMTLLIALSY
                     LALVAYVQAGRRGWVLVYALAVLASVYTEYSASYALLPQGLVLVYLLRHERRRAGVLI
                     AAAGCAALAFLPWLPQLLHTAASGPNKALFAVTPVRVASSLLSLAGLAEPLSQRGTAA
                     EATASLSAGDLALYGLLALAVTPAALTGAVMLARRSRLGLPVAVALLAGTIVTGVVLS
                     LVFPGYAKRTVLPALLGWALLIGAAPFAAGLPGRWRALSLGSTALALLIAAVTLTATY
                     RDGDKQHWRDLAAATMQVQAAGGTVVTYPTVAATLIGVYEPVVMTRPHVAIRDGGRLP
                     ALTAPGGARPDRVWLAYLDFQGIEAVRADLAAQGYVRRSHTEYPDGLYLDQYTPSGSG
                     AP"
     gene            complement(1802..>2356)
                     /locus_tag="VF276_04970"
     CDS             complement(1802..>2356)
                     /locus_tag="VF276_04970"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX6606250.1"
                     /translation="PAIAQEAQALLTGGYFRIYTNTDLIGVELAGALKNIIALGAGIA
                     DGLQAGDSTKAAFITRGLAEIARLGVAAGANLFTFAGVAGVGDLIATCASPFSRNRRL
                     GAALVAGQTVEQFHQETGQVAEGVPTTRAVRALAAQYGVDMPITEEMYQILFAGKHPL
                     LALAALMERDPTDELRGMGVPGSE"
BASE COUNT          356 a          861 c          838 g          301 t
ORIGIN      
        1 gtctgcgtgg accgcgcggt ggccgcctcg ggcctgcaac cggaacagat cgatgtggtg
       61 ttgcgcaccg gcggctcgtc ctccatcccc gccttcgtgc gcctgctgga gaggcggttc
      121 ggcccggcca agatccgcaa gcaggatgtg ttcaccggca tcgccgccgg gttgggcatc
      181 gccggctacc gcagcctgct gcccccggta gcgcgcaccg cgtagcccga cgcgctcgcc
      241 cgccgtccat gcctatattg gaacggtcag ggtgcgcctg acccggacgg cgtatactga
      301 tccaggtaga gcccgtcggg atactcggtg tggctgcggc gcacatagcc ttgcgcggcc
      361 agatcggccc gcacagcctc gattccttgg aagtccaggt aggccagcca gactctgtcg
      421 gggcgcgcgc cgcccggcgc ggtcagggcc ggcagccgcc cgccgtcgcg gatggcgaca
      481 tgcggccggg tcatgacgac cggctcgtac acgccgatca acgtggcggc gacggtgggg
      541 taggtgacga cggtgccgcc cgcggcctgg acctgcatgg tggccgccgc caggtcgcgc
      601 cagtgctgtt tgtcgccatc ccgatacgtt gccgtgagcg tgaccgccgc gatcagcagc
      661 gccagcgcgg tactgcccag gctcagcgcg cgccaccggc ccggcagccc ggcggcaaag
      721 ggcgccgcgc cgatcagcag cgcccagccc agcagcgcgg gcagcactgt gcgcttggcg
      781 tagcccggaa agaccaggct caagaccacc ccggtcacga tggtgccggc cagcagcgcg
      841 acggcaaccg gcagccccaa tcgcgagcgg cgagccagca tcacggcccc ggtcagcgcc
      901 gcgggcgtca cggccagtgc cagtagcccg tagagtgcga gatcgcccgc ggacagactc
      961 gccgtcgctt ccgccgctgt gcctctctgg cttagcggct cggccagccc ggccagcgac
     1021 agcagcgaac tggcaacccg caccggcgtg acggcgaaca gcgccttatt ggggccgctt
     1081 gccgccgtat gcagcaactg gggcaaccag ggcagaaagg ccagcgcggc gcaccccgcc
     1141 gccgcgatca gcacccccgc ccgccgccgt tcgtgccgta gcagataaac cagcaccaga
     1201 ccttgcggca gcagcgcgta gctggcgctg tactcggtat agacgctcgc cagcacggcc
     1261 agggcgtaga ccaggaccca gccccggcgc cccgcctgca cgtaggccac cagcgcgaga
     1321 tacgacagcg cgatgagcag cgtcatgagc gcgtagggcc ggccctcctg cgagtaccag
     1381 acgtgcagcg gcgcaacggc cagccccagc gccgcgacca cccccgccca cggcgcgacc
     1441 agccgggccc cgagccggta gaccacggcg agggtcagcg tccccgcgat cacgctgacg
     1501 agcctcccgg cgtcggtctc cggcgccagc agactcatcg ccttggtgag cgcgaagtag
     1561 agcgggggat gcacctcgta ggcgccgtgc aggcccagca catcaggcca cggcagccgg
     1621 ctgacgccga tggtgatccc ctcgtcgacc cacaggctga gcgccgtgta gcggtagagc
     1681 cgcagcaccc cgcccagcac gacgaccgcc agggccacgg cgccaggggt cagccgccag
     1741 gggacggcgg tggcgggtgc ggcgggccgg ggggcggtgt tgatcacttg ctgggacacg
     1801 cttactcgct gccgggcacg cccatgccgc gcagttcgtc ggtggggtcg cgctccatga
     1861 gggcggcgag ggccagcagc gggtgcttgc ctgcaaagag gatctgatac atctcctcgg
     1921 tgatcggcat atcgacgccg tactgcgcgg ccagggcgcg gacggcgcgc gtggtcggca
     1981 cgccctcggc cacctggccg gtctcctggt ggaactgctc gaccgtctgg ccggcgacca
     2041 gggcggcacc caggcggcgg ttgcggctga acgggctggc gcaggtggcg atgaggtcgc
     2101 ccaccccggc caccccggcg aaggtgaaca ggttggcgcc cgccgccacg cccagccggg
     2161 cgatctcggc cagcccgcgc gtgatgaacg ccgccttcgt gctgtcgccc gcctgcaagc
     2221 cgtcggcgat gcccgcgccc agggcgataa tgttcttcag cgccccggcc agttccacgc
     2281 cgatgaggtc ggtgttggtg tagatgcgga aatagccgcc ggtcagcagg gcctgggcct
     2341 cctgggcgat ggccgg
//
LOCUS       DASUYC010000307         2736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1641219, whole genome shotgun sequence.
ACCESSION   DASUYC010000307 DASUYC010000000
VERSION     DASUYC010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1641219"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VF276_04975"
     CDS             complement(<1..543)
                     /locus_tag="VF276_04975"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX6606251.1"
                     /translation="MPIGTMHVMSRGLGRPTPYTIILPEPDVGPGPYPVLLQLHGKWD
                     DYQSWLVKSRLASHVESLPLIVVLPDGGNFWWSNVGTYLAYEDYLAQDLRAHIANTYP
                     VRPGPWALGGLSMGGFGSVHLALKHPDLYCSVFAHSSPFFTTEELREYEPQFSAATVA
                     DLDCYRLATAANTDRLPVLSF"
     gene            complement(638..1849)
                     /gene="ccsB"
                     /locus_tag="VF276_04980"
     CDS             complement(638..1849)
                     /gene="ccsB"
                     /locus_tag="VF276_04980"
                     /inference="COORDINATES: protein motif:HMM:TIGR03144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEX6606252.1"
                     /translation="MEEAVFGFNEWTLTTTSWVCYFGALLSFIGLLIAQATQLSRQRI
                     LAREAAEFGGVQPVFAGAAGAGTATLAAPVMGRSRGTPRAAETAATVPMGASARLFGR
                     AGLGLTIAGSATLGIALVMRSVHSGHIPYMSLYEICLMFTFGIAMAYLLIGELWLKAR
                     SIGAFALLFVYLIQSYALLLIPPDLKTARPLVPALQSSWLPVHVSMAIVAYSCFAVAA
                     GAAIMYLLKYHEVGGWTRALPSLAAIDEFMFRAVTVGFPFQTLLLITGAYWAQTAWSK
                     AWSWDPKEVWALITWLAYAAFLHVRVQRGWRGTTMAWMALAGFAVVMITFIGVNYLVS
                     WFQLDSMHAYAGDMPGFLGFGIFGVVLVLLLGSIMYGEMRRRRLAAAGRARHAARQAE
                     AATESPASAGN"
     gene            complement(1854..>2736)
                     /locus_tag="VF276_04985"
     CDS             complement(1854..>2736)
                     /locus_tag="VF276_04985"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HEX6606253.1"
                     /translation="MGHGTDFTVRQSNFRIEYFPGTTDVKEYWCDLTITKNGEDVLHK
                     TIRVNDPLRYDNINFFLVQYQQVATVRAIDKSGSALPMNKMGQNGPITTTTEAALQPM
                     LLSFQMTDNDNLPMDLVQVKRPGKDTVTLQVSNYTSTPRGDNENPPLFVRAFIGKNFD
                     QILYNDFIPRQGPLVIPEIPDIAFDFKRDTATIMEVAEDYGLTPVAVWFVIMTAGFAL
                     SLYITFVRCWARITPNAADPAQSDVLLGGLAEKNKVTFEGEFEKLALRVRDYLARRVD
                     TAAGPRATPEMPPGLES"
BASE COUNT          495 a          890 c          920 g          431 t
ORIGIN      
        1 gaagctcaac acgggcagcc ggtcggtatt ggccgcggtc gccaggcgat agcaatcgag
       61 atcggccacc gtggcggcgg aaaattgcgg ctcgtactca cgtagctctt ccgtggtgaa
      121 gaacgggctg gagtgggcga acaccgaaca atagagatcc gggtgcttga gcgccaggtg
      181 caccgagccg aagccgccca tcgacagccc gcccagggcc caggggccgg gccgcaccgg
      241 gtaggtattc gcgatgtgcg cgcgcaggtc ctgggccagg tagtcctcgt aggccaggta
      301 cgtgcccacg ttgctccacc agaaattgcc gccgtccggc agcacgacga tcagcggcag
      361 cgactcgacg tggctcgcca ggcgcgactt caccagccac gactggtagt cgtcccactt
      421 gccgtgcagt tgcagcagca ccgggtacgg ccccggcccc acgtcgggct cgggcaggat
      481 aatcgtgtag ggcgtggggc gccccagccc ccggctcatc acgtgcatgg ttccaatagg
      541 catccgactt cacctttccg gcctggcgtg gctcagacag gccatgaaac aaaacgagtc
      601 cgccgctggg gcggactcgt agagggcgca aatgggatca gttgccggcg gaagccggcg
      661 actcggtggc ggcctcggcc tggcgggcgg cgtggcgggc gcggcccgcg gcggcgaggc
      721 ggcggcggcg catttcgccg tacatgatgc tgcccagcaa cagcacgagc accacgccga
      781 agatgccgaa gcccaggaag cccggcatgt cgcccgcgta ggcatgcatg ctgtccagtt
      841 ggaaccagct caccaggtag ttcacgccga tgaaggtgat catcaccacg gcgaagccgg
      901 ccagcgccat ccaggccatc gtcgtgccgc gccagccgcg ctgcacgcgc acgtgcaaga
      961 aggcggcgta ggccagccag gtgatcagcg cccagacttc cttcgggtcc cagctccagg
     1021 ccttggacca agccgtttgc gcccagtagg cgccggtgat cagcagcagc gtctggaacg
     1081 ggaagccgac ggtgacggcg cggaacatga actcgtcgat ggccgccagc gacggcagcg
     1141 cccgcgtcca gcccccgacc tcgtgatact tcagcaggta catgatcgcc gcgcccgccg
     1201 ccacggcgaa gcagctatag gcgacaatcg ccatcgacac gtggacgggc agccagcttg
     1261 actgcagcgc gggcaccagc gggcgggccg tcttcaggtc gggcgggatc agcagcagcg
     1321 cgtacgactg gatcaggtag acgaacagga gcgcgaaggc gccgatgctg cgcgccttga
     1381 gccagagttc gccgatcagc aggtaggcca tggcgatgcc gaaggtgaac atcagacaga
     1441 tctcgtacag gctcatgtac gggatatggc cgctgtgcac cgagcgcatc accagggcga
     1501 tgcccaacgt ggccgacccg gcaatcgtca agcccaggcc cgcccgcccg aagaggcgcg
     1561 cgctcgcgcc catcggcacc gtcgccgccg tctccgcggc gcgaggggtg ccacggctgc
     1621 ggcccatgac gggtgcggcc agcgtggcag tcccggcgcc ggcggccccg gcaaacacgg
     1681 gttgcacccc gccgaactcc gccgcctcgc gggccagaat ccgctgccgc gaaagctgcg
     1741 tcgcctgggc gatgagcagc ccgatgaacg acaacagcgc gccgaagtaa cagacccacg
     1801 acgtggtggt cagggtccac tcgttgaaac caaacactgc ttcttccatc gtgttaagac
     1861 tccaatcctg gtggcatctc cggcgtcgcc cgcgggccgg cggccgtgtc tacccggcgg
     1921 gcgaggtaat cgcggacgcg cagggccagt ttctcaaact ccccctcaaa ggtgactttg
     1981 ttcttctcgg ccagtccgcc cagcaacaca tcgctttgcg cgggatcggc cgcattgggc
     2041 gtgatccgcg cccagcagcg gacgaaggtg atatacagag acaacgcgaa accggcggtc
     2101 ataatgacga accagacggc gaccggggtc aggccgtaat cctcggcgac ctccatgatg
     2161 gtggccgtgt cgcgcttgaa gtcaaaggcg atgtcgggga tctcggggat gaccagcggc
     2221 ccctggcgcg ggatgaaatc gttgtagagg atctggtcga agttcttgcc gatgaaggcg
     2281 cgcacaaaga gcggcgggtt ctcgttgtcg ccacgcggcg tgctggtgta gttgctcacc
     2341 tgcagggtca cggtgtcctt gccgggccgc ttcacctgca caaggtccat cggcaggttg
     2401 tcgttgtcgg tcatctggaa ggacaacagc atcggctgca aggccgcttc cgtcgtcgtc
     2461 gtgatcggcc cgttctggcc catcttgttc atcggcagcg cgctgcccga cttgtcgatg
     2521 gcccgcaccg tcgccacctg ctggtattgc accaggaaga agttgatgtt gtcgtagcgc
     2581 aacgggtcgt tgacgcggat cgtcttgtgc agcacgtcct cgccgttctt agtgatggtc
     2641 aggtcgcacc agtactcctt cacatcggtg gtgccgggga agtactcgat gcggaagttg
     2701 ctctggcgca cggtgaagtc ggtgccgtgg cccatc
//
LOCUS       DASUYC010000308         7966 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_489265,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000308 DASUYC010000000
VERSION     DASUYC010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 7966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7966
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489265"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1218)
                     /locus_tag="VF276_04990"
     CDS             complement(<1..1218)
                     /locus_tag="VF276_04990"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX6606254.1"
                     /translation="MSAVGTPPPAPGQTPSGGPRRPGWGEPDAPAIGDWLYIGADETV
                     EVYCGKAEVGQGVRTSLSQAVAEELRVPVAQVRMVLGDTARTPFDAGTFGSRTTPYMA
                     PQLRQVAASARELLLDLAASRWQVDRATLVAAGGQVTHPPTGRALSYGALTGGRRLTE
                     PWREDAPLTPATAWTVAGHPAPKVDGRAFVTGRHRYTPDLTRPGMLAGAVLRPPAFRA
                     TLRTLDTQAAEAISGVTVVRDGDFVGVTAPDRATARRACAAIEAEWDVPPQIDAPELY
                     DYLRTHPAPVTEGGWGGPTAYTHGSVAAGRAGAAHTLAATYTVAYIAHTPMETRAALA
                     EWEGDHLTVWTGTQRPFGVRGELAAAFELAEDQVRVIVPDTGAAYGGKHSGEAAVEAA
                     RLARGAGRPVKLIW"
     gene            complement(1215..1709)
                     /locus_tag="VF276_04995"
     CDS             complement(1215..1709)
                     /locus_tag="VF276_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX6606255.1"
                     /translation="MARVTELTVNGAPVAVDVDATTPLLSVLREDLGLTGSKIGCGEG
                     ECGACTVLAGDRAIRACITPVSTVADRPIVTIEGLAQGDRLHPLQQAFLDAEAFQCGY
                     CTPGMIMAGLGLLTACPNPTEAEIVAGMQGNICRCGTYRRIIRAIQQAAEERAAARAG
                     EVLP"
     gene            complement(2002..3261)
                     /locus_tag="VF276_05000"
     CDS             complement(2002..3261)
                     /locus_tag="VF276_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HEX6606256.1"
                     /translation="MSARLPAPPEVERLAAEPVPPPGMPRGPGLVIILGALSAFGPLS
                     IDMYLPALPALGRDFGAGASETQLTLSACLVGLALGQMIGGPISDALGRRRPLLIGVA
                     AYALASLLCVVAPSIGALVGLRVVQGFAGAVGIVIARAVVRDLYAGVALARFFSLLML
                     VNGLAPILAPVIGAQLLRFTSWRGVFVTLGVAGALLFFAATRLVETLPPASRQTGGIP
                     ATLTTFCRLLTDRSFVGYALAGGLAFAGMFSYISGSPFVLQGIYGVSPQGFSLFFSTN
                     ALGLVVAGQINGRLVGRVPVRRLLALGLIGTASGGMALLAVVLLGAGLVGILPALFLV
                     VASLGFVFPNATTLALAGHPRTAGSASALLGVLQYAVGAVAAPLAGLSTGLSALPMAV
                     VIAAMSLSALLTFLLLPSRGARTSAEC"
     gene            complement(3279..6056)
                     /locus_tag="VF276_05005"
     CDS             complement(3279..6056)
                     /locus_tag="VF276_05005"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX6606257.1"
                     /translation="MADLLNTQMGQYRLTEVIRRGGMATVYKAYQESLDRYVAIKVLF
                     HNEDPQFAMRFKREARAMAHLQHPNILPIYDHGEQDGMLFLVLQYVERGVTLSDMLGK
                     PMEPVPALRLVARVLDALDYAHQRGIVHRDIKPGNILMPAPNWPMLADFGIAKLLTEG
                     ENLNLTSPSLIIGTPAYLAPEQALGQPIDGRTDLYSTGVVLYQMVTGHVPFDSGTPVV
                     VLTKQAYEAPPPPTMFNPDLPPTVALAVLRALEKDPAHRYQSAAEMAQALEQIAADLD
                     SARPAPALVESRPAPGAPSELHSHYLAGVQAFAAQRWDQAAHSLRQVVALDPTYENAA
                     RLLDAALAALQTPGAATVVRPPGTSPAPAESQAAPISAPPPVPAESQAAPVPPPTAAA
                     AAAPDTRSPQAIPVGEPAPPVAQPLSMAGPDLAPASPPARRPRRRAASVPPETAGPGT
                     QPAVAVPPPDMETAPVPAASPERARQAAPVAESPVAAAVPAQPGGVVGTVDTSPAPAR
                     RSVWPFVATGAGLLLLALAVLFGPGLFASRAGGTGGGSPTPAPATVVATPDAAATLSA
                     APVVVPPASPPATTPPTAAVPTATPAPPAATPTTAALLATTEQGAIGAVRFADGQKVV
                     DQLQITATLSTLPARPVGKGLFAWMQDSASGTTVNLGRVVPDDAGLWRLTYSDPAQAN
                     LSGRFDGFLVTYEALETDPTTPSSEAVLRGQLPVQALVHIRHLLFSYPDTPEQVGLEV
                     GLRAQVAALAQHAQLLDDAQKAGSLAKVKLEAEQIANLIEGNQGPDFGDLNKDGKVDN
                     AGDGFGLLVGGSQPGYIQDAKDHAELSATAPDATENIKQHAAGVIATADNVQARVTTI
                     RDQALAMLRTPTIAEARPLVATAVKLAGEALNGVPTADGKVLPVANSGGALTGYDEAL
                     LMATMPIQVVK"
     gene            complement(6341..7486)
                     /locus_tag="VF276_05010"
     CDS             complement(6341..7486)
                     /locus_tag="VF276_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005269488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX6606258.1"
                     /translation="MQNTAEDRTLNPFPWYRRMRETDPIHFYPEYRAWQVFRYDDVLR
                     VLSDSAAFSSNISGGHERQDPLAASLVTTDPPRHRQLRNLVTQAFTPRSVAALANRIT
                     TIVNDLLDRVVAQGQMDVVGDLAYPLPVIVIAEMLGIPREDRDRFKAWSDAIVGASMG
                     GGNPQAEMSVYFLEMIEQRRQEPRDDLISALLNAQIEGVHLTRRELLGFCVLLLVAGN
                     ETTTNLIGNAVLCFDEHPQILAELRAAPALIPGAIEEVLRFRSPVQMMYRRVAADVDL
                     DGRPLRAGQMVMAQIGSANRDAAQFPDPDRFDIRRVPNRHLAFGHGIHFCLGAPLARL
                     ESKIALTMMLDRLPEMRRVPSVALQGTGSPIVYGVKHLPITFHARAA"
     gene            complement(7681..>7966)
                     /locus_tag="VF276_05015"
     CDS             complement(7681..>7966)
                     /locus_tag="VF276_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6606259.1"
                     /translation="ERNVAVQTIKSIAYKPWMAEEHTRSTWYKPLENQTDIDAAVWWV
                     LARPGIFLNTVGDINLLPRVLDAASRFDPAMTQAALEPRVAQLSFEPLFV"
BASE COUNT         1185 a         2836 c         2808 g         1137 t
ORIGIN      
        1 ccagatcagc ttgaccgggc ggcccgcacc ccgcgccagc cgcgccgcct ccaccgccgc
       61 ctcgccgctg tgtttgccac cgtaagccgc gccggtgtcg ggcacgatca cccgcacctg
      121 gtcctcggcc agctcgaagg ccgcggccag ttcgccgcgc acgccgaagg gccgctgggt
      181 gccggtccag accgtcaggt ggtcgccctc ccactcggcc agggcggcgc gggtctccat
      241 cggcgtatgg gcgatgtagg ccacggtgta tgtcgcggcc agcgtgtgag cggcacccgc
      301 ccggcccgcg gccaccgagc cgtgggtata ggcggtgggg ccgccccagc cgccttccgt
      361 caccggggcc gggtgcgtgc gcaggtaatc gtacagttcc ggcgcgtcga tctgcggcgg
      421 cacgtcccac tcggcctcga tggcggcgca ggcgcggcgg gccgtcgcgc gatccggcgc
      481 ggtgacgccg acgaaatcgc cgtcgcgtac caccgtcacg ccggagatcg cctccgccgc
      541 ctgcgtgtcc agcgtgcgca gggtggcgcg gaaggcgggc gggcgtagca cggcccccgc
      601 gagcatccct ggccgggtca ggtcgggggt gtagcggtgg cgcccggtca cgaaggcgcg
      661 cccgtctacc ttgggcgccg ggtgcccggc cacggtccac gccgtggccg gggtcagcgg
      721 ggcgtcctcg cgccacggct cggtcaggcg ccgcccgccg gtcagcgcgc cgtagctgag
      781 cgcgcgcccg gtgggcgggt gcgtcacctg ccccccggcg gccaccagcg tcgcccggtc
      841 cacctgccag cgggaagcgg ccaggtccag caggagttcg cgcgccgagg ccgccacctg
      901 gcgcagttgc ggcgccatgt agggcgtcgt gcggctgccg aaggtgcccg cgtcgaacgg
      961 ggtgcgcgcc gtgtcgccca gcaccatgcg cacctgcgcc accggcacgc gcaactcctc
     1021 agccaccgcc tggctcagcg aggtgcggac gccctgcccc acctcggcct tgccgcaata
     1081 gacctcgacg gtttcatccg cgccgatata cagccagtcc ccgatggccg gggcatcggg
     1141 ctcgccccag ccgggccggc gcgggccgcc cgaaggtgtc tgccccggtg cgggcggggg
     1201 cgttcccacg gcgctcatgg cagtacctcc cccgcccgcg ccgccgcccg ttcctcggcg
     1261 gcctgttgaa tggcgcggat aatgcgccgg taggtgccgc agcggcagat gttgccctgc
     1321 atcccggcca cgatctcggc ctcggtgggg ttagggcacg cggtcaacag gcccagcccg
     1381 gccatgatca tccccggcgt gcagtagccg cactggaacg cctcggcgtc gagaaacgcc
     1441 tgctgcagcg ggtgcagccg gtcaccctgc gccagccctt caatcgtcac gatgggccgg
     1501 tcggccaccg tgctcaccgg cgtgatgcac gcgcggatag cccggtcccc ggccaggacg
     1561 gtgcaggccc cgcactcgcc ctcgccgcag ccgatcttgc tcccggtcag ccccaggtcc
     1621 tcgcgcaaca cgctcaacag cggcgtggtc gcgtccacgt ccaccgccac cggcgcgccg
     1681 ttaaccgtca actccgtcac tctcgccatg atctgatgct cctctatcgg gcgctctccg
     1741 gcggggaacc attccccgac ccggcatggg ccgcgtgcgc ctgggggaaa ccgatcagga
     1801 atcgtgccgc cacggagcgg tgcgatccct tcccgcgccg gatcttctcg tcgccactca
     1861 gtagtctgtc gcgcggtgtt tgtcactctg agcgcagcaa agagtctcgt cttggacccc
     1921 accgagacga gattcttcgc tccgctcaga atgacagtgc tcgtgtgacg accaactcag
     1981 cactcactca gcactcagca ctcagcactc agcactcgtc cgggcgccac gcgacggcag
     2041 cagcaggaag gtcagcaggg cggacaggct catcgccgcg atgaccacgg ccatgggcag
     2101 cgcgctcagc cccgtgctca gcccggccag cggcgcggcg acggctccga cggcgtattg
     2161 cagcacgccc agcagcgccg aggcactccc cgcggtgcgc gggtggccgg ccagcgccag
     2221 cgtcgtggca ttggggaaca cgaagcccag gctggcgacg accaggaaca gcgcgggcag
     2281 gatgcccacc agaccggcgc cgagcaggac caccgccagt aacgccatcc cgccgctcgc
     2341 cgtgccgatc agccccagcg ccagcagccg gcgcaccggc acacgcccga ccagccgccc
     2401 gttgatttgc cccgccacca ccagccccag cgcgttcgtg ctgaaaaaga ggctgaaccc
     2461 ctggggcgag acgccgtaga tcccctgcaa cacgaacggg gaacccgaga tgtaggagaa
     2521 catcccggca aaggccaggc cgcccgccag cgcgtagccg acgaacgagc ggtcggtcag
     2581 cagccggcaa aaggtggtca gggtggcggg gatgccgcct gtctgacgcg acgcgggcgg
     2641 cagcgtctcg accagccgcg tggcggcgaa gaatagcaag gcgcccgcga cacccaaggt
     2701 gacgaacacg ccgcgccagg aggtgaagcg cagcagttgc gcgccgatca cgggcgcgag
     2761 aatgggcgcc agcccgttca ccagcatcag cagcgaaaag aagcgcgcca gggccacgcc
     2821 ggcatacaga tcgcgcacga cggcgcgggc gatgacgatg ccgacggcgc cggcgaagcc
     2881 ttgcaccacg cgtagcccca ccagcgcgcc gatagagggg gccaccacgc acaacagcga
     2941 ggccagggcg taggcggcca ccccgatcag cagcgggcgg cgccggccca gcgcgtcgct
     3001 gatcggcccg ccgatcatct gcccgagggc caggcccacc aggcacgcgc tcagcgtcag
     3061 ttgtgtctcc gacgcgcccg caccgaaatc gcgccccagc gcaggcaacg ccggcaggta
     3121 catgtcgatg gacaggggac cgaatgcgct cagggcgccc aggatgatca ccagccccgg
     3181 cccgcgcggc atccccggtg ggggcaccgg ctcagcggcg aggcgttcga cttcgggcgg
     3241 ggcgggcagg cgtgcgctca taacccggcg ccgggcgcct acttcaccac ctggatcggc
     3301 atggtcgcca tgagcagcgc ctcgtcgtag ccggtgaggg cgccgccgct gttcgccacg
     3361 ggcagcacct tgccgtccgc ggtgggcacg ccgttcaacg cctcgcccgc cagttttacc
     3421 gctgtggcga ccaggggccg cgcctcggcg atggtcgggg tcctgagcat ggctagcgcc
     3481 tggtcgcgga tggtcgtgac ccgcgcctgg acgttatccg ccgtggcgat tacccccgcc
     3541 gcgtgctgtt tgatgttctc ggtcgcgtcg ggtgcggtcg ccgacaactc ggcgtggtct
     3601 ttagcgtcct ggatgtagcc gggctgcgag ccgcccacca gcaggccgaa accgtccccg
     3661 gcgttgtcga ccttgccgtc cttgttcagg tcgccgaagt ccggcccctg gttgccttcg
     3721 atcaggttgg cgatctgctc ggcttccagc ttcacttttg ccaggctccc ggccttttgc
     3781 gcgtcgtcga gcaactgggc gtgctgggcc agggccgcca cctgggcgcg caagcccact
     3841 tcgaggccca cctgctcggg tgtgtccggg taggagaaca acagatggcg gatatggacc
     3901 agcgcctgca ccggcaactg cccgcgcagc accgcttcgc tggacggcgt ggtgggatcc
     3961 gtttcgagcg cctcgtacgt caccaggaag ccgtcgaagc gcccgctcag gttggcctgg
     4021 gccgggtcac tataggtgag gcgccagagg ccggcgtcat ccgggaccac gcggcccagg
     4081 ttcacggtcg tgccgctggc gctgtcttgc atccaggcga acaacccctt gcccaccggc
     4141 cgcgcgggca gcgtgctcag cgtcgccgtg atctgcaatt gatcgaccac cttctgtccg
     4201 tcggcgaagc gcacggcgcc gatagcgccc tgctcggtgg tcgccaggag ggccgccgta
     4261 gtaggggtgg cggccggtgg cgcgggcgtc gccgtgggca ccgccgcggt cgggggggtg
     4321 gtcgccgggg gcgatgccgg cgggacgact accggcgccg cggaaagcgt ggccgccgcg
     4381 tcgggtgtgg caaccacggt ggccggcgcg ggggtcggcg aaccgccacc cgtgccgccc
     4441 gcgcgcgacg cgaacagccc cggcccaaag agcacggcca gggccagcag caagagaccc
     4501 gcgccggtgg cgacgaaggg ccacacgctg cgccgcgcgg gtgccggcga cgtgtccacc
     4561 gtgcccacga ccccgcccgg ctgcgcgggc acagccgccg ccaccgggct ctcggccacc
     4621 ggcgcggcct ggcgggcgcg ctccggtgag gcagccggca ccggcgcggt ttccatatcg
     4681 ggcggcggca ccgccactgc cggttgtgtg cctgggccgg cggtctccgg cggcacgctc
     4741 gccgcgcggc gacgcggacg gcgcgcgggt ggggaagccg gggcgagatc ggggccggcc
     4801 atggagagcg gttgggccac aggcggcgcc ggctccccga caggtatcgc ctggggcgag
     4861 cgggtgtccg gcgccgccgc agcggcggcg gtgggcggcg gcaccggcgc ggcctgggac
     4921 tcggcgggta cggggggcgg cgcggatatc ggcgcagcct gggattcggc gggcgcgggc
     4981 gatgtgcccg gcgggcggac taccgtggcg gcgcctgggg tctgcaatgc ggccagcgcc
     5041 gcgtccagca gccgcgccgc attttcgtag gtcgggtcca gggccaccac ctgccgcagg
     5101 ctgtgggccg cctggtccca gcgctgcgcc gcgaaggcct gcaccccggc cagatagtgg
     5161 ctgtgcaatt cgctcggtgc gcccggcgcc gggcgggatt cgaccagggc cggggcgggc
     5221 cgggcgctat ccaggtcggc ggcgatctgt tcgagcgcct gagccatttc cgccgcgctt
     5281 tgatagcggt gggccgggtc tttctccagc gcgcgcaaca cggccagggc gacggtgggc
     5341 ggcagatcgg ggttgaacat ggtgggcggc ggcggcgcct cgtacgcctg cttggtgagc
     5401 accaccaccg gcgtgcccga gtcgaacggc acatgccccg tcaccatctg gtagagcacc
     5461 accccggtcg agtacagatc ggtgcgcccg tcgatgggct ggcccagggc ttgctccggc
     5521 gccagatagg cgggagtgcc gataatcagg ctgggcgagg tcaggttcag gttctcgccc
     5581 tcggtcagca gcttggcgat gccgaagtcg gccagcatcg gccagttggg ggccggcatc
     5641 aagatattgc ccggcttgat gtcccggtgc acgatgccgc gctggtgggc atagtcgagg
     5701 gcgtcgagca cgcgcgccac caggcgcagc gccgggaccg gctccatcgg cttgcccagc
     5761 atgtcgctca gcgtcacccc ccgctccaca tattgcagca ccaggaagag catgccatcc
     5821 tgctcgccgt ggtcgtaaat cggcaggatg ttggggtgtt gcaggtgcgc catcgcccgc
     5881 gcctcgcgct tgaagcgcat ggcgaactgc gggtcctcgt tgtggaacag caccttgatc
     5941 gccacatagc gatccagcga ttcctggtac gccttgtaga cggtcgccat gcccccgcgg
     6001 cggatgacct ccgtgagccg gtattggccc atttgtgtat tgagcagatc agccatcgcg
     6061 ggtgctcctg tgcgacgcgg gtactacgag gcgggcgggg ctgtcaccaa cgcccccgga
     6121 tccctcctcc ggtgggcaca gcgctcggcc gtagcggcta gtgcgagggt aggatgcaaa
     6181 ccggttcgat cagaccatca ttgctgcgct ataaagagtc tcgttcaatt atacaacgat
     6241 ccactacggc ataccacaca atacaatgca aatgccggga aagggtaaca gggtggaagc
     6301 aaaatacgcc aatttgccat cacatttgcg cccggcgcgc ctatgccgca cgcgcgtgga
     6361 acgtgatcgg caggtgcttc acaccgtaga cgatagggct acccgtgccc tgcagcgcca
     6421 cgctgggcac ccggcgcatc tccggcagcc ggtccagcat catcgtcagc gcgattttgc
     6481 tctccagccg cgccagcggc gcccccaggc aaaagtggat gccgtggccg aaagccaggt
     6541 gccggttggg cacgcgccgg atatcaaacc ggtcgggatc ggggaactgc gccgcgtccc
     6601 ggttcgcgga cccgatctgc gccatgacca tctgccccgc ccgcagcggc ctgccgtcca
     6661 ggtccacgtc ggcagccacc cggcggtaca tcatctgcac gggcgaccgg aagcgcagca
     6721 cctcttcgat ggcccccgga atcagggccg gggcggcgcg caactcggcc aggatctgcg
     6781 ggtgctcgtc gaagcagagc accgcgttgc cgatcaggtt ggtcgtcgtc tcgttgccgg
     6841 ccaccagcag caacacgcag aagccgagca gttcgcggcg ggtcagatgc acgccctcga
     6901 tttgcgcgtt cagcagcgcg ctgatcaggt cgtcgcgcgg ctcctggcgg cgctgctcga
     6961 tcatctctag aaagtagacg ctcatctcgg cctggggatt gccgccgccc atgctcgcgc
     7021 ccacgatggc gtccgaccag gctttgaagc ggtcgcggtc ctcccgcggg atgcccagca
     7081 tttcggcaat gacgatgacg ggcagcgggt aggccaggtc gcccacgaca tccatctgcc
     7141 cctgggcgac cacgcgatcc agcaggtcgt tgacgatggt ggtgatgcgg ttcgccagcg
     7201 cggccacgct gcgcggcgtg aaggcttgcg tcaccaggtt gcgcaactgc cggtggcgcg
     7261 gcgggtcggt cgtgaccagg ctggcggcca gtgggtcttg ccgctcatgc cccccgctga
     7321 tgttggacga gaaggcggcg gagtccgaca gcacgcgcaa gacatcgtca tagcggaaca
     7381 cctgccaggc ccggtactcg ggatagaaat ggatcgggtc ggtctcgcgc atccggcgat
     7441 accaggggaa aggattgagc gtgcggtctt cggcggtgtt ttgcatagca ggatggcccc
     7501 ttgtctagag gcagcggggc gtggtgccgg ccgcacgcac cacaaccccc tacgccccaa
     7561 cagggcggcg gcaaccacgt tagtattccg ccgccctgca ctatcccaca aaccgatctc
     7621 cccttcccgc ggcgggaaga gggccggggg gttatgcgca gcaggtcccg cacctccccg
     7681 ctacacgaac agcggctcga agctcaactg cgccacgcgc ggctccaggg cggcctgcgt
     7741 catggccggg tcgaagcggc tcgcggcgtc gagcacgcgg ggcagcaggt tgatatcgcc
     7801 cacggtgttg aggaagatgc cgggccgcgc cagcacccac cagaccgcgg cgtcgatgtc
     7861 ggtctggttc tccagcggct tgtaccacgt cgagcgggtg tgttcctcgg ccatccaggg
     7921 cttgtaggcg atggacttga tcgtctgcac cgccacgttg cgctcc
//
LOCUS       DASUYC010000309         3145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1555888, whole genome shotgun sequence.
ACCESSION   DASUYC010000309 DASUYC010000000
VERSION     DASUYC010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1555888"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VF276_05020"
     CDS             <1..332
                     /locus_tag="VF276_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX6606260.1"
                     /translation="LEEAARIDGCSEPGIFWRIILPLSKPAIGALAIFTFVRSWNAFL
                     WPLIVLQKTRTFTLPVGVASLQNEFAVDYGLIFAGAAIAAVPMILFFLAFQRYFMEGV
                     RMGALKG"
     gene            334..1575
                     /locus_tag="VF276_05025"
     CDS             334..1575
                     /locus_tag="VF276_05025"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019003853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEX6606261.1"
                     /translation="MADPMRVLGLMSGTSVDGIDAALCEFAPDPTGGPGALAFRLLHF
                     QEMPLPAAIRQRVLALCRPETSRIDDLTEMSFVLGDLFAEAALAACAAAGPAPGDLDL
                     IASHGQTVYHLVEPGRRIATLQIGQPAVIAARTGVTTIADLRVADVAAGGEGAPLVSF
                     FDALFFRHPTHHRALQNIGGIGNVTFVAPDGTATAFDTGRGNSLIDYSARRYSGGARS
                     YDEDGEMARAGRVDATLLAELLAEPYYQMPPPKTTGRELFGDAYGAGVVARAEAAGLA
                     PADVVATLTALTARTIADAYARFGPAAGPGEMIVAGGGARNPALLDMLRAALPPGVPI
                     ALHDAYGLPAKAKEATVFALLGYAGLHGCPATLPSCTGATRPAVPGAITPGANYRRLL
                     ARVAATPTEELCPIQSLRLIS"
     gene            1617..2447
                     /gene="murQ"
                     /locus_tag="VF276_05030"
     CDS             1617..2447
                     /gene="murQ"
                     /locus_tag="VF276_05030"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEX6606262.1"
                     /translation="MTPLELARVINEADATVAPAVARQLPQIAAAIAAITDRLAAGGR
                     LIYMGAGTSGRLGVLDASECPPTFNTPPEQVIGWIAGGPAALTTSIEAVEDDPDLGRA
                     DAARLQIGAGDVLVGIAASGRTPYVLGALTYAREVGALTIALACNADPPIGRAAEITI
                     AVETGPEIVNGSTRLKAGTAQKMVLNMLSTGVMIRLGKTYGNLMVDVQLTNAKLRRRA
                     VAIVETVTGLSTEDAAALLAQAGDTKTALVAALAGVPPAIARERLRAAGGVVRRALEG
                     "
     gene            2450..>3145
                     /locus_tag="VF276_05035"
     CDS             2450..>3145
                     /locus_tag="VF276_05035"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEX6606263.1"
                     /translation="MSALYLGVDGGGTSTRAVIVDAGGQECGRGHAGSANHTAVGLDA
                     AVANIRQAVVQAARAAGQDPPLAGAWYGLAGVDRPLDKQWLLPHLADLAAHTRLTNDA
                     ELGLAALPETCGVCLIAGTGSIAVGRDRTGRVARAGGWGHVFSDEGSGYAIGRAALQA
                     VSRAADGRGPATALTAAILAAWDLPEPSAIIAHVYLAGEKAVIAALAPLVLQHAGDDA
                     VARRIVVEAANELT"
BASE COUNT          430 a         1121 c         1150 g          444 t
ORIGIN      
        1 agttggagga ggcggcgcgc atcgacgggt gcagcgaacc gggcattttc tggcggatta
       61 tcctgccgct gtcgaagccg gctatcggcg cgctggcgat tttcacgttt gtgcgctcgt
      121 ggaacgcctt cctgtggccg ctgatcgtgc tgcaaaagac gcgcaccttc accctgccgg
      181 tgggcgtggc gagcctgcaa aacgagttcg ccgtggacta cggcctgatc ttcgccgggg
      241 cggccatcgc cgccgtaccg atgatcctct tcttcctggc cttccagcgc tatttcatgg
      301 aaggcgtgcg catgggcgcg ctcaagggtt agcatggccg atccgatgcg cgtgctgggg
      361 ctgatgtcgg gcacgtcggt ggacggcatc gacgcggcgc tctgcgagtt tgcgcccgac
      421 cctaccggcg gccccggcgc gctggcgttc cggctgctgc atttccagga gatgccgcta
      481 ccggcggcga tccggcaacg ggtgctggcg ctctgccgcc cggaaaccag ccggatcgac
      541 gacctgaccg agatgagctt cgtgctgggc gacctgttcg ccgaggcggc gctggccgcc
      601 tgtgcggcgg cggggccggc ccccggcgat ctcgacctga tcgcctccca cggccagacg
      661 gtctaccacc tcgtggagcc gggccgccgg atcgccacgc tgcaaatcgg ccagcccgcc
      721 gtgatcgcgg cgcgcaccgg cgtgacgacg attgccgacc tgcgcgtggc cgacgtggcc
      781 gccgggggcg agggcgcgcc gctggtgtcg ttcttcgatg cgctgttttt ccgccacccc
      841 acccaccacc gggcgctgca aaacatcggc ggcatcggca acgtgacctt cgtcgcgccc
      901 gacggcacag ccacggcctt cgacaccggg cggggcaaca gcctcatcga ctactccgca
      961 cgccgctaca gcggcggcgc ccggtcctac gacgaagacg gcgagatggc ccgcgccggg
     1021 cgggtggacg ccacgctgct ggccgaactg ctggccgagc cgtactacca gatgccgccg
     1081 ccgaagacga cgggccgcga gttgttcggc gacgcctatg gcgcgggtgt ggtcgcgcgc
     1141 gccgaggcgg cgggcctggc ccccgccgac gtggtggcga ccctgaccgc gctgaccgcg
     1201 cgcaccatcg ccgatgccta cgcccgcttc ggccccgccg ccgggcccgg cgagatgatc
     1261 gtggccgggg gcggggcgcg caatccggcg ctgctggaca tgctgcgcgc ggccctgccg
     1321 cccggcgtcc ccatcgcact ccatgatgcc tacgggctgc cggccaaagc caaggaggcc
     1381 accgtgttcg ccctgctggg ctacgcgggc ctgcacggct gcccggctac cctgccctct
     1441 tgcaccggcg ccacccggcc cgccgtgccc ggcgccatca cgcctggcgc caactaccgc
     1501 cgcctgctgg cgcgggtcgc cgccaccccc acggaggaat tgtgtccgat tcagagcttg
     1561 cgcttgatca gctagcgacc gagggcatca acccggccag caccggcatc gaccggatga
     1621 cgccgctgga gctggcccgc gtgataaacg aagccgacgc gaccgtcgct ccggcggtgg
     1681 cccggcaact gccgcagatc gccgccgcca tcgccgccat caccgaccgg ctggccgccg
     1741 ggggccggct catctacatg ggcgcgggca cctcgggccg cctcggcgtg cttgacgcct
     1801 cggagtgccc gccgacgttc aacacgccgc cggaacaggt catcggctgg atcgccgggg
     1861 ggccggcggc gctgaccacc tcgatcgaag cggtggagga cgaccccgac ctgggccggg
     1921 ccgacgctgc ccgcttgcag atcggcgccg gggatgtgct ggtcggcatc gcggccagcg
     1981 ggcgcacgcc ctacgtgctg ggcgcgctga cctatgcccg cgaggtgggc gccttgacca
     2041 ttgcgctggc ctgcaacgcc gacccgccca tcggccgcgc cgccgagatc acaatcgccg
     2101 tggagaccgg acccgaaatc gtcaacggct cgacgcggct gaaagcgggc acggcgcaaa
     2161 agatggtcct gaatatgctg agtacgggcg taatgatccg cctgggcaag acctacggca
     2221 acctgatggt ggacgtgcag ctcacgaacg ccaagctgcg ccggcgcgcg gtcgccatcg
     2281 tggaaacggt gacggggctc tcgacggaag acgcggcggc gttgctggcc caggcgggcg
     2341 acacgaaaac ggccctggtc gcggcgctgg ccggggtgcc gccggcgatc gcccgcgaac
     2401 ggttgcgggc ggcgggaggg gtggtgcgcc gcgcgctcga aggataagca tgagtgcctt
     2461 gtatttaggg gtggacggtg ggggaaccag cacgcgcgcc gtgatcgtcg atgccggcgg
     2521 gcaggaatgc gggcgcggcc acgccggcag cgccaaccac accgccgtcg gtctcgacgc
     2581 cgcggtggcg aacatccgcc aggccgtggt gcaggcggca cgggcggcgg gccaggatcc
     2641 gccgctggcc ggggcctggt acggcctggc cggtgtggat cgccccctgg ataagcagtg
     2701 gttgctgccc cacctggccg acctcgccgc gcacacccgc ttgaccaacg atgccgaact
     2761 cggcctggcc gcgctgccgg agacgtgtgg cgtttgcctg atcgccggga cgggcagcat
     2821 cgctgtgggc cgggaccgca ccgggcgggt ggcccgtgcg ggcggttggg gtcatgtgtt
     2881 cagcgacgag ggcagcggct acgccatcgg gcgggcggcg ttgcaggccg tctcgcgcgc
     2941 cgccgacggc cgtggcccgg ccacggccct gacggccgcc atcctggcgg cctgggatct
     3001 gcccgagccg agcgcgatca tcgcccatgt ctatctggcg ggcgagaagg ccgtcatcgc
     3061 cgcgctggcg ccgctggtct tgcagcacgc gggggacgat gccgtggccc ggcgcatcgt
     3121 ggtggaggcg gcgaacgaac tgacg
//
LOCUS       DASUYC010000310         2221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_884131,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000310 DASUYC010000000
VERSION     DASUYC010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884131"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..>2221
                     /locus_tag="VF276_05040"
     CDS             161..>2221
                     /locus_tag="VF276_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606264.1"
                     /translation="MARLRLPVALALLLALVAGGAVFAVYRLVPPAWTVEIGGPLSGA
                     LLDSGFYADEAEQAGRYRWTHGQATLTFADIGADSPLVLRFRASAPARPADAPPPVLT
                     LTVASQVVATYTLAAQPAVYRTPPIPRPPGEPGLQATFSIPTFTPGNGDTRDLGMKLE
                     WVRVEPAPGAPAPTPPAAVLLEAVLIALGLYGALARPSLGRSILAALVALVALGLLVA
                     GLALARPLLTPWLPEIALAAAGLGLLVVGWPVWRRWPAGVDSLAAPRSVAAPLILAVI
                     LLGYAAFALNVIPQIDWIGHADYADNAVVARNIAAGRGPVIDYVPQFYRFFPGITHPA
                     ETWPLLQPLLITPFFLLLGAQTWVAKLPNLFVLLALAVAVYHFAGRWWDRRVALLAAV
                     LTLLHPYFFQTVLYPINDLSFTLLALLTIGWAWEALEATVVRARRQVALAAAEEDGAA
                     PGARRPPPVSALPPASPTRPAILAGIAAGLLIWSKGPSAGLIIVGLLLGLAWGWRTGR
                     WAGRRPPRDWQPLLWGLGSAALVVAPLLLRNFLTFGKPFHSTEEYDLWILRFWTGGTG
                     LPWENIYADYLDGRPLPDRSLLLHSYQELYTAVRWGFDQIWTGGIVHGDIVDLPVFVA
                     GLAGWLLIPARLRGLRTAWVGAFVVYGLFVLLAWHYEPRYFLALAPWFYLGTAAALFW
                     IWDHL"
BASE COUNT          237 a          862 c          767 g          355 t
ORIGIN      
        1 ccagccacgc caaacccccg ctggacggcg tgacgctgcc cctgccccac ccggtgacgg
       61 aagggcgcat cgacggcgcc gtggccgcgc agcgccgccc ggaacaggtt gtcatccccc
      121 acctcgcgcc gggcacgccg gtgcgaatcg acgcgcgatg atggcccggt tgcgcctgcc
      181 ggtcgccctg gccctgctgc tggcgctggt cgcgggcggc gcggtgtttg ccgtctatcg
      241 cctcgtcccg cccgcctgga cggtcgagat cggcggcccg ctgagcggcg cgctgctcga
      301 ctccggcttc tacgccgacg aggccgagca ggccgggcgc taccgctgga cgcacggcca
      361 ggccacgctg accttcgccg acatcggcgc ggacagcccg ctggtgctgc gcttccgcgc
      421 ctccgcgccc gcccgcccgg cggacgcccc accgcccgtg ctgacgctca ccgtcgccag
      481 ccaggtggtc gccacctata ccctggccgc gcagcccgcc gtctacagaa ccccacccat
      541 cccgcgcccg cccggcgagc ccggtttgca agccacgttc agcatcccca ccttcacccc
      601 cggcaacggc gacacgcgcg acctgggcat gaagctggaa tgggtgcgcg tggaaccggc
      661 ccccggcgcg cccgcgccca cgccgcccgc cgccgtgctg ctcgaagccg tgctgatcgc
      721 gctcggcctc tacggcgccc tggctcgccc gtcgctcgga cgcagcatcc tggccgcgct
      781 cgtggcgctg gtcgcgctgg ggctgctggt ggccgggctg gcgctggccc gcccgctgct
      841 cacaccctgg ctgccggaga tcgcgctcgc ggcggcgggg ctaggcctgc tcgtggtggg
      901 ctggccggtg tggcggcgct ggcccgcggg cgtggatagc ctggctgcgc cgcgctccgt
      961 ggccgcgccc ctcattcttg ctgtgattct actgggatat gccgcctttg ccctgaacgt
     1021 gatcccgcag atcgactgga tcggccacgc cgactacgcg gacaacgccg tggtggcgcg
     1081 caatatcgcg gcggggcgcg gcccggtcat cgactacgtg ccgcagttct accggttctt
     1141 ccccggcatc acccacccgg cggagacctg gccgctgttg caaccgctgc tgatcacgcc
     1201 gttcttcctg ctgctgggcg cgcaaacctg ggtcgccaag ctgcccaacc tgttcgtgct
     1261 gctggccctg gccgtcgccg tctaccactt cgccgggcgc tggtgggacc ggcgcgtggc
     1321 cctgctcgcc gccgtgctga cgctgctgca cccctacttc ttccagacgg tactctaccc
     1381 gatcaacgac ctgagcttca cgctgctggc cctgctcacc atcggctggg cctgggaggc
     1441 gctggaagcg accgttgtgc gcgcccgccg gcaggtcgca ctggccgccg cggaggagga
     1501 cggggccgcg cccggtgccc gccgcccgcc gccggtctcg gcgctgccgc ccgcgtcgcc
     1561 cacccgcccg gccatcctgg cggggatcgc cgccgggctg ctcatctgga gcaaggggcc
     1621 gagcgcgggc ctcatcatcg tgggactgct gctggggctg gcctggggct ggcgcaccgg
     1681 gcgctgggcc gggcgccgcc cgccgcgcga ctggcagccg ctgctgtggg gcctggggag
     1741 cgcggcgctg gtggtagcgc cgctgctgct gcgcaacttc ctcacctttg gcaagccgtt
     1801 tcactccacc gaggaatacg acctctggat cttgcgcttc tggaccggcg gcaccggcct
     1861 gccctgggag aacatctacg ccgactacct ggacggccgc ccgctgcccg accgcagcct
     1921 gctgctgcac agctaccagg aactgtacac ggcggtgcgc tggggcttcg accagatctg
     1981 gaccggcggc atcgtccacg gcgacatcgt cgatctgccg gtgttcgtgg cagggctggc
     2041 gggctggctg ctgatcccgg cccggctgcg cgggctgcgc acggcctggg tgggcgcctt
     2101 cgtggtctac ggcctgttcg tgctgctggc ctggcactat gagccgcgct attttctggc
     2161 cctggcgccc tggttctacc tgggcacggc ggcggccctg ttctggatct gggaccacct
     2221 g
//
LOCUS       DASUYC010000311         4998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_766066,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000311 DASUYC010000000
VERSION     DASUYC010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766066"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1151..2026
                     /locus_tag="VF276_05045"
     CDS             1151..2026
                     /locus_tag="VF276_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX6606265.1"
                     /translation="MIPSTFDYSVPTTLAEAVALLGQNPDAKILAGGHSLIPLMKFRL
                     AAPALLVDINRIEGLDYIREEDGRLAIGALTRESALDHSGLIREKYPLLADTVKMIAD
                     PLVRNMATVGGNLAHADPANDHPATMLAYGAQVVATGPNGERVIPIEGLFEGLFQTSL
                     AHDEVLTEIRIPAPRPRSGGAYVKVERKVGDFATVAVAVQITLDEQGNCESVGIGLTN
                     VGETPIKATQAEQALLGKPLDDANIHVAAQLAAEAAEPVGDQRGSEEYKRALVKTLMV
                     RALRKAAQRATGEGA"
     gene            2034..2516
                     /locus_tag="VF276_05050"
     CDS             2034..2516
                     /locus_tag="VF276_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX6606266.1"
                     /translation="MSTHHVRVTVNGEVKEADIESRLLLVHFLRDVLNLTGTHIGCDT
                     TSCGACSVLLNGRAVKSCTVYAVQANGGEVLTVEGLSRNGALHPIQEAFTEKHGLQCG
                     FCTPGMMISAVALLERNPHPTEEEIRWGLSGNLCRCTGYVNIVKAVQQAADKMSEVAG
                     "
     gene            2535..4883
                     /locus_tag="VF276_05055"
     CDS             2535..4883
                     /locus_tag="VF276_05055"
                     /EC_number="1.2.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic carbon-monoxide dehydrogenase large
                     subunit"
                     /protein_id="HEX6606267.1"
                     /translation="MTAVHGLGHSLRRKEDPRFIRGKGNYIDDIKLPDMLYLDIVRSP
                     YAHARITNINSEPALQVPGVLAVITGKDLDKYGLAWMPTLMSDRQMVLPVDTVKYQAQ
                     EVAAVVATSRYAAADGVAAVEVDYEPLPVVVDPFQALEPGATKVRDDKENNHIWHWEV
                     GDQAATEQAFADAEVTVKQDIYIPRIHVSSIETCGCIASWDPIEEKLTMYMTTQAPHA
                     VRTVIALVAGHLGLAEHKIRVISPDIGGGFGGKVPVYPGYVIAIAASVLIGKPVKWIE
                     DRSENLQADSFARDYHISAEVAGKRDGTLTAVRVKSVADHGYTDAAANPSKFPAGLFH
                     VCTGSYNFSNAFVEVDGVYTNKPPGGIAYRCSFRITEAVHTIERMADIMAHELGMDPA
                     EFRMKNFIQPEQFPYRSALGWEYDSGNYGAALRKAMDMVGYEELRKEQAEKRARGELM
                     GIGISSFTEAVGAGPSHTFDILGLKMFDSCEIRIHPTGKAIARFGTKSQGQGHETTYA
                     QILAEELGIPAGDIKVEEGDTDTAPYGLGTYASRSTPTAGAAGALAARKIREKAKAIA
                     AHLLEVAEEDLVWEDGKFQVKGSPGHAKTMQEIAFAAYTNHPPGMEAGLEAVSYYDPP
                     NLTYPFGSYICVVDIDRGTGQVNVRRFVAVDDCGVRINPMIVEGQIQGGLTMGLAPAL
                     YEEISYDADGNIYGGTFMDYLLPTAVETPKWEIGETVTPCPHHPQGAKGVGESATVGA
                     PAAVVNAVVDALWHLGVRNIDIPMTAPKVWALLKEKGAALEE"
BASE COUNT          951 a         1672 c         1581 g          794 t
ORIGIN      
        1 gccacggcgg cgcccgtcgc gtcgattacc tggagtttga gaaacgacgt gcccaggtcg
       61 atgcccagga agtgggtcgt tggtgtggtt tgcatggtgt ggttcctgct cctggccgcg
      121 ggccggttgc aacccggccc ctcagtagtc tgtcacctgt atcttgtcat tctgagcgga
      181 gcgaagaatc tcgtctcgtc gcccaaaaga ggagactctt cgctgcgctc agagtgacaa
      241 actcgctgcg accgattact cagcaaggat ggatgatacg ccaaggctgc atgatgtatg
      301 cctgtagttg gggctgccca gcactggcac ccgcaagcac ccaccaggcg ggccggcggg
      361 cgcgacccgc ccggccgctg ggtgcccaac cgggccgtgg caagacccgg tggtccccgc
      421 caccagcgca cgctgcatgg ccctcgatac accatgagaa gtggctaagg gctacctgct
      481 gagatcgtta tagtgcttgc agccccaggc cggatatgct atagtggttt tgctaccatt
      541 cagcctctgc tgaggcacga caccggcatt gatgtagacg atgacgccat gtctagatcg
      601 gttcgccgtc gcgattgcgg ccctgggtgc gcgggcgcgc ccggtgcagg ccgcgctatt
      661 gcttccgctg ttcgatgtgg tcgtccagcc ggaaccgcct cttgcccgca cgttccgccc
      721 cttgctcagc gtaccgcgcg cctcaccgag gtggacgcct gcccgaggac gaccacgcac
      781 accgcgcatc accctccggc ccgtgacggc ttcccgcagc agcttcgttc ctccgatagc
      841 cgttccggcc cgctccctgc cggcgcacca ggctaccctg gttacatctt cctgcctgcc
      901 cgcgcaggtt gaatagttac tacgggcacg gacacgaact caacggatca ggagttacgc
      961 ggaaccgacc cgcgtagaaa gctgcgttat catgccgcgc tcagcatccg ccgccctggc
     1021 gactcgcgcc ccacccgatg ccccgccgcg cccggccggg cgcacaatcg cccgcgtcac
     1081 ctgacccctg atagccgatc tctattcacc ggcaagtaag cacacggacg tgcaatgaag
     1141 gagatcatgt atgattccat cgactttcga ctacagtgtg cccaccaccc tcgcggaagc
     1201 ggtggcgctc ctggggcaaa acccggacgc caagattctc gccggcggtc acagcctcat
     1261 cccgctgatg aaattccggt tagccgcgcc cgcgctgctg gtggacatca atcgcatcga
     1321 ggggttggac tacatccgcg aggaggatgg ccggctggca atcggcgcgc tgacgcgcga
     1381 gagcgccctc gaccactccg gcctgatccg cgagaagtac ccgctgctcg ccgacacggt
     1441 caagatgatc gccgacccgc tggtgcgcaa tatggcaacc gtgggcggca acctggccca
     1501 cgccgacccg gccaacgacc acccggccac gatgctggcc tacggcgcgc aggtggtggc
     1561 gaccggtccc aacggcgagc gcgtcattcc catcgagggc ttgttcgagg gcctgttcca
     1621 gaccagcctc gcccatgatg aagtcctgac cgaaattcgc atcccggcac ctcggccccg
     1681 cagtggcggc gcctatgtga aagtcgagcg caaagtcggc gacttcgcca ccgtcgccgt
     1741 cgccgtgcag atcactctgg atgagcaggg gaactgcgag tcggtgggca ttggcctgac
     1801 caacgtcggg gagacgccga tcaaggcgac ccaggccgaa caggcgctgc tgggcaaacc
     1861 gctggacgac gccaatatcc acgtggcggc gcaactggcc gccgaggccg ccgaaccggt
     1921 cggcgaccag cgcggctcgg aagaatataa gcgtgccctg gtgaagaccc tgatggtccg
     1981 cgccttgcgc aaggccgcgc aacgcgcgac aggagaagga gcgtagatta cccatgagca
     2041 cccatcacgt gcgcgtcacc gtcaacggtg aggtcaagga agccgacatc gagtcgcgcc
     2101 tgctgctcgt ccatttcctg cgcgatgtcc tgaatctcac cgggacgcat atcggctgcg
     2161 acaccacgtc gtgcggcgcc tgctcggtcc tgctgaacgg ccgggccgtc aagtcgtgca
     2221 ccgtctacgc cgtgcaggcc aacggcggcg aggtgctgac cgtcgaaggg ctgagccgca
     2281 acggtgccct gcacccgatt caggaagcct tcacggaaaa gcatggcctg cagtgcggct
     2341 tttgcacgcc gggcatgatg atctccgcgg tcgctttgct ggaacgcaac ccgcacccca
     2401 ccgaggaaga gattcgctgg ggcctatcgg gcaacctgtg ccgctgcacc gggtacgtca
     2461 atatcgtgaa ggccgtccaa caagcggcgg acaaaatgag cgaagtcgcc ggttagccgg
     2521 cagaggagga cgccatgacg gcagttcacg gtttgggcca cagcctcaga cgcaaagaag
     2581 acccgcgctt catccgcggc aaggggaatt atatcgacga tatcaaactg ccggacatgc
     2641 tctacctgga catcgtccgc agcccgtatg cccacgcgcg gatcacgaat atcaacagcg
     2701 agccggcgct ccaggtgccc ggcgtcctcg ccgtcatcac cggcaaggac ctggacaagt
     2761 acgggttagc ctggatgccc acgctcatga gcgaccggca gatggtcttg ccggtggaca
     2821 cggtgaagta ccaggcgcag gaggtcgccg ccgtggtcgc caccagtcgc tacgccgccg
     2881 ccgacggcgt cgcggcggtc gaagtcgact acgaaccgct gccggtggtc gtggacccgt
     2941 tccaggcgct ggagccgggc gcgaccaaag tgcgcgacga caaggagaac aaccacatct
     3001 ggcactggga ggtgggcgat caggcggcga ccgagcaggc gtttgccgat gccgaagtca
     3061 ccgtcaagca ggacatctac atcccccgaa ttcacgtctc gtcgattgag acctgcggct
     3121 gcatcgccag ttgggacccg atcgaggaaa agctgaccat gtacatgacg acccaggcgc
     3181 cgcacgccgt ccgcaccgtc atcgcgctgg tggccgggca cctgggcctg gccgaacaca
     3241 agatccgcgt gatttcgccg gacatcggcg gggggttcgg gggcaaggtg ccggtctatc
     3301 ccggttacgt cattgccatc gccgcctcgg tgctcatcgg caaaccggtc aagtggatcg
     3361 aggatcgctc tgagaacctg caggccgact cgttcgcccg cgattaccac atcagcgccg
     3421 aagtcgccgg caagcgcgac ggcacgctga ccgcggtgcg cgtcaaaagc gtggccgacc
     3481 acggctacac cgacgccgcc gccaatccgt ccaagttccc cgccggcctc ttccacgtct
     3541 gcaccggctc gtataacttc agcaacgcct ttgtcgaggt ggacggcgtc tataccaaca
     3601 agccgcccgg cggcatcgcc taccgctgct cgttccgcat caccgaggcg gtgcatacca
     3661 tcgagcgcat ggccgacatt atggcccacg agctgggcat ggacccggcg gagttccgca
     3721 tgaagaactt catccagccc gagcagtttc cctaccgttc ggcgctgggc tgggagtacg
     3781 acagcggcaa ctacggcgcg gccctgcgca aggcgatgga catggtcggc tacgaggagc
     3841 tacgcaagga gcaggccgag aagcgcgccc gcggcgagtt gatgggcatc ggtatctcct
     3901 cgttcaccga ggccgtgggg gccgggccct cgcacacgtt cgacatcctc ggcctgaaga
     3961 tgttcgactc gtgcgagatc cgcatccacc ccaccggcaa agccatcgcg cgcttcggca
     4021 cgaaatcgca gggccagggc cacgagacga cttacgcgca gatcctcgcc gaagaactcg
     4081 gcatccccgc gggcgacatc aaggtcgagg aaggcgacac cgataccgcg ccctatggcc
     4141 tgggcaccta tgcgtcgcgc agcaccccca ccgccggggc ggcaggcgca ctggccgcgc
     4201 gcaaaatccg cgagaaggcc aaagcgatcg ccgcgcacct gttggaagtg gcggaggaag
     4261 acctggtctg ggaagacggc aaattccagg tgaagggttc gcccggccat gccaagacga
     4321 tgcaagagat cgccttcgcc gcgtacacca atcacccgcc gggcatggag gcggggctgg
     4381 aggcggtgtc ctactacgat ccacccaact tgacctatcc cttcggctcc tacatctgcg
     4441 tggtggacat cgaccggggc acgggccagg tcaacgtgcg gcgcttcgtg gcggtggacg
     4501 actgcggcgt gcgcatcaac ccgatgatcg tggagggcca gatccagggc ggtctgacga
     4561 tgggcctggc gcccgccttg tacgaggaaa tctcctacga tgccgacggc aacatctacg
     4621 gcggcacgtt catggattac ctgctgccga ccgctgtcga aacgccgaag tgggagatag
     4681 gcgagacggt cacgccctgc ccgcaccacc cgcaaggcgc caagggcgtc ggcgaatcgg
     4741 ccacggtcgg cgcgccggca gcggtggtca atgcggtggt ggacgcgctc tggcatctcg
     4801 gcgtgcgcaa catcgacatc ccgatgaccg cgcccaaggt gtgggcgctg ctcaaagaga
     4861 agggagcggc actggaggaa tgaggcaact cagccgtcaa ctatcagcta gtagctcgcc
     4921 accggattct gatgagcggc aggccggttt caaagcggcc cctacaccgc aaactcatca
     4981 gggtcggcta ctagctga
//
LOCUS       DASUYC010000312         3455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1246030, whole genome shotgun sequence.
ACCESSION   DASUYC010000312 DASUYC010000000
VERSION     DASUYC010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246030"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..149)
                     /locus_tag="VF276_05060"
     CDS             complement(<1..149)
                     /locus_tag="VF276_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004190903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HEX6606268.1"
                     /translation="MAALKGSKTEDNLKAAFAGESQANRRYLYFANKADVEGQNDVAA
                     LFRSTA"
     gene            393..1010
                     /locus_tag="VF276_05065"
     CDS             393..1010
                     /locus_tag="VF276_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6606269.1"
                     /translation="MAAVLKDPGALPAPAPIHQLHHYAYRARDAEETRHFYEDILGLP
                     LYHIIQSDYVPSTGEYCPYTHFFFRLQDGSFIAFFDLGDDQACEPSPNTPKWVNHISF
                     RVDSIQALQDMKARLEAHGVEVLGVTDHHIFHSIYFFDPNGVRLELTAQLADEFQMLQ
                     ESKTAHARLKEWTARKEQWRADRAAGKAAAPLKPQQNDRPEFVKK"
     gene            1086..2810
                     /locus_tag="VF276_05070"
     CDS             1086..2810
                     /locus_tag="VF276_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6606270.1"
                     /translation="MADRYAEVAHTNGYELTSGTGYQLPEYPFRAPPEVASGQTGHHP
                     IVIVGGGLTGLTAACALAQYGVPAVLLDEDNTVGVKGASSRGICYTQKSLEIFERLGI
                     YDRIAAKGIQWSIGRTFAGEDEVYSFDLRQQGAFNLSTQPPFINIQQFYIEAFLVDRI
                     YELGQVDLRWRNRVTGFRQDGEAATLSVSTPAGDYELRADHVIDATGCHSPFREWVGA
                     SVTAKKGSDRWCIADVRFSKHPPVERHTWVEAPFNDNRAVWQHLMADNVWRIDYQMPP
                     DCDPAEVSREDVVRERLTRQFGPDAQIEIVWVGPYAYRSECVDRMRHGRVFLMGDAAK
                     VVSPFGARGGNTGIADADNLAWKLAAVLHGRAAPALLDSYHEERHEAATQNVLVTSRT
                     TRFLRPATPMEKLFRDAALGLARKHAFARQFVNTGRMAVANPYTRSGACEATGGLSVQ
                     NVAFAWPDGSRGTVNDLLRWAGRNLLLLVFGGTSPGALARLQGLAQTAPVRCVQVLGP
                     DDAPGALEHVRDPRGHLQGACHVFGHAWALLRPDSYVAATGESVDASLVHAVGRALGT
                     TLEEEPNA"
     gene            2807..2995
                     /locus_tag="VF276_05075"
     CDS             2807..2995
                     /locus_tag="VF276_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2783 domain-containing protein"
                     /protein_id="HEX6606271.1"
                     /translation="MKTLLNQVDADAFYEQLLDAHEGLDEKQSALLNARLILLLANQV
                     GDPKVLKDCVEAARQLPA"
     gene            3012..>3455
                     /locus_tag="VF276_05080"
     CDS             3012..>3455
                     /locus_tag="VF276_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX6606272.1"
                     /translation="MSTEQPGLNSLSKSFEPAAIEAKWGPLWEQRGYARAGWRGTGEA
                     KPGEPSFAIQLPPPNVTGTLHMGHAFNQTIMDSLARYHRMRGFNTLWVPGTDHAGIAT
                     QIVVERQLQEQGTSRHDLGREAFVRKVWEWKQKSGNTITTQMRRMG"
BASE COUNT          579 a         1243 c         1150 g          483 t
ORIGIN      
        1 gccgtggagc ggaacagcgc cgccacgtcg ttctggcctt ccacgtcggc cttgttcgcg
       61 aagtacaggt agcggcgatt ggcctgggac tcgcccgcaa aggcggcctt caggttgtct
      121 tccgtcttgg agcctttgag tgctgccatg gagatctcct tctggtgaat gaagagggga
      181 agctggcgcc ccggccgggc cggggacgca gcaagcgtac tgagcgtgga gggtggcgtc
      241 taatagggtt gctcaatgca ctccatgccc gagggctatt gccgccccgg tgtcgataaa
      301 tggtcctaag gggcgcggcc cggccgcgtt ggagcggccg gagcgttacg gtatagtaaa
      361 tgcattacgc ataggtaaac acggagactc ccatggccgc cgttctcaag gacccgggcg
      421 ccctgcccgc ccccgccccg atccaccagc tgcaccacta cgcctaccgc gcgcgcgacg
      481 ccgaggagac gcggcacttc tacgaggaca tcctcggcct gccgctgtac cacatcatcc
      541 agagcgacta cgtgccgagc acgggcgagt actgcccgta cacccacttc ttcttccgcc
      601 tgcaggacgg ctcgtttatc gccttcttcg acctcggcga cgaccaggcc tgcgagccct
      661 cgcccaacac gcccaagtgg gtcaaccaca tctccttccg cgtcgactcc atccaggcgc
      721 tgcaggacat gaaggcccgg ctggaagcgc atggcgtcga ggtgctgggc gtcaccgacc
      781 accacatctt ccacagcatc tatttcttcg accccaacgg cgtgcgcctg gagctcacgg
      841 cccagctcgc cgacgagttc cagatgctgc aggagagcaa gaccgcccat gcccggctga
      901 aggagtggac cgcgcgcaag gagcagtggc gcgccgaccg cgccgccggc aaggccgccg
      961 cgccgctgaa accgcagcag aatgaccgcc cggagttcgt gaagaagtga cttcgtcacc
     1021 ccgggcaggc gggcgccctg ggttcccgcc gccgcgggaa tgacgcgatc ggaggagaca
     1081 aggacatggc ggaccgctac gccgaggtgg cccacaccaa cggctatgaa ctcacttcgg
     1141 gcacgggtta ccagctgccc gaatatccct tccgcgcgcc gcccgaggtc gcgtccgggc
     1201 agaccggcca ccaccccatc gtgatcgtcg gcggcggcct gaccggcctc accgccgcct
     1261 gcgcgctggc gcagtacggc gtgccggccg tgctgctgga cgaggacaac acggtgggcg
     1321 tgaagggcgc atcctcgcgc ggcatctgct acacgcagaa gtcgctggag atcttcgagc
     1381 ggctgggcat ctacgaccgc atcgccgcca agggcatcca gtggagcatc gggcgcacct
     1441 tcgccggcga ggacgaggtg tactccttcg acctgcggca gcagggcgcc ttcaacctgt
     1501 cgacgcagcc gcccttcatc aacatccagc agttctacat cgaggccttc ctcgtggacc
     1561 gcatctacga gctgggccag gtcgacctgc gctggcgcaa ccgcgtcacc gggttccggc
     1621 aggacggcga ggccgccacc ctgtcggtga gcacgcccgc cggcgactac gaactgcgcg
     1681 cggaccacgt gatcgacgcc accggctgcc acagcccctt ccgcgagtgg gtgggcgcgt
     1741 ccgtcacggc gaagaagggc tccgaccgct ggtgcatcgc cgacgtgcgc ttcagcaagc
     1801 accctcccgt ggagcgccac acctgggtcg aggcgccgtt caacgacaac cgcgccgtct
     1861 ggcagcacct gatggccgac aacgtctggc gcatcgacta ccagatgccg cccgactgcg
     1921 accccgcgga ggtcagccgc gaggacgtcg tgcgcgagcg actgacgcgc cagttcgggc
     1981 ccgacgccca gatcgagatc gtgtgggtgg gcccctatgc ctaccgcagc gaatgcgtcg
     2041 accgcatgcg ccacgggcgc gtgttcctga tgggcgacgc cgccaaggtg gtcagcccct
     2101 tcggcgcgcg cggcggcaac acgggcatcg ccgacgccga caacctggcg tggaagctcg
     2161 ctgccgtcct gcacgggcgc gccgcgcccg ccctgctgga cagctaccac gaggaacgcc
     2221 acgaagcggc gacgcagaac gtgctggtca ccagccgcac cacgcgtttc ctgcggcccg
     2281 ccacccccat ggagaaactg ttccgcgacg cggccctggg cctcgcgcgc aagcacgcct
     2341 tcgcccgcca gttcgtcaac accggccgca tggcggtggc caacccctac acccgctccg
     2401 gtgcctgcga ggcgaccggc ggcctgtccg tgcagaacgt cgccttcgcc tggccggacg
     2461 gcagccgggg cacggtgaac gacctgctgc gctgggccgg ccgcaacctg ctgctgctgg
     2521 tgttcggcgg cacctcgccc ggcgcgctgg cgcgcctgca ggggctggcc cagacggcgc
     2581 cggtgcgctg cgtgcaggtg ctgggccccg acgacgcgcc cggtgcgctg gagcacgtgc
     2641 gcgatccgcg gggccacttg cagggcgcct gccacgtgtt cggccacgcc tgggcgctgc
     2701 tgcgtccgga cagctacgtg gccgccacgg gcgagagcgt cgacgcctcg ctggtgcatg
     2761 cggtcggccg cgcgctcggc acgaccctgg aagaggagcc caacgcatga agaccctgct
     2821 gaaccaggtg gacgccgatg ccttctacga gcagctgctg gacgcccacg aagggctgga
     2881 cgagaagcag tcggcgctgc tcaacgcccg gctcatcctg ctgctcgcca accaggtcgg
     2941 cgaccccaag gtgctgaagg actgcgtgga ggccgcccgg cagttgcccg cgtgaaccgt
     3001 aaaatcccgg ggtgagcacc gaacaacccg ggctgaacag cctttccaag tccttcgaac
     3061 cggccgccat cgaggcgaaa tggggccccc tgtgggaaca gcggggctac gcccgcgccg
     3121 gctggcgcgg caccggcgag gcgaagccgg gcgagccgtc cttcgcgatc cagctgccgc
     3181 caccgaacgt gacgggcacc ctgcacatgg ggcacgcgtt caaccagacc atcatggaca
     3241 gcctggcgcg ctatcaccgc atgcgcggct tcaacacgct gtgggtcccc ggcaccgacc
     3301 acgccggcat cgcgacgcag atcgtggtgg agcgccagct gcaggaacag ggcacgagcc
     3361 ggcacgacct cggccgcgag gcgttcgtgc gcaaggtctg ggagtggaag cagaagtcgg
     3421 gcaacaccat caccacgcag atgcgccgca tgggc
//
LOCUS       DASUYC010000313         3544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1069233, whole genome shotgun sequence.
ACCESSION   DASUYC010000313 DASUYC010000000
VERSION     DASUYC010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069233"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="VF276_05085"
     CDS             complement(<1..939)
                     /locus_tag="VF276_05085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6606273.1"
                     /translation="MDARPRPWVGYILRSYPRLSQTFILNEILALEQRGVRIVIFAGT
                     DPREPVVQPEVAQVRAPVHYLEGATQRPAALIAAEHLRLALAAPRRYVRALRYVLAHR
                     DIDTGYHAASRFACFAQAVYLARLLRVEQRRQGRAAGHLHAHFAHDPTLIAVLAQMLT
                     GLPYSFTAHARDLYQIPPAALIDRVRRARTVVTCCAANIDYLQETIPPALQAKVRLIH
                     HGVDLGRFRPAPAASKEAAPAANQPLLLSVGRLVEKKGFPDLLAACAQLKQAGYRFRC
                     AIYGEGPLEAELAATIARLDLGDTVELAGVCPQHELV"
     gene            complement(1026..2219)
                     /locus_tag="VF276_05090"
     CDS             complement(1026..2219)
                     /locus_tag="VF276_05090"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX6606274.1"
                     /translation="MAERSDDPAPAAGNDIQETVIARVMTSTSMDKHPTAFYTTGLPV
                     LQVALNVEAMTRHLAPWLASLVPPGHTPVVGYAKLIAYKQGNRGLIHYEVTSREGGPR
                     RLVFGKLYPAANQAERVEATMRPLWAGVFGGRTDVGIPQPLGVIPDLSMLVYLPAEGR
                     FLDEVILDDEAVRYMDLAGIWLGLLHQSRLPLERRFQPATEIANLHAWAALIGHKYPD
                     EAEDALRISQLLQDALPNLGFAAEAPIHKDFHYGHIVVGDGLKIIDFDEMRLGDPNFD
                     LAHFCANLYLLAQRKNMPPRRCAELQSVFVRAYARQTGWTANPRFVYFYGYTCLKIAK
                     QLCTRRGLRPRPEGEEQDRQLRLMVAHGLTALGYDVGRQMSGTFATLIMSKPGALRPD
                     GAAEK"
     gene            2640..>3544
                     /locus_tag="VF276_05095"
     CDS             2640..>3544
                     /locus_tag="VF276_05095"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX6606275.1"
                     /translation="MDDLINTQLGQYRLTEVIRRGGMSTVYKAYQESLDRYVAVKVLF
                     HGQNPQFVARFQREARAIAHLQHPNILPIFDYGEQDGLLYLVLQYIEQGMTLGDLLGR
                     PMEPVPALQLMGRVLAALDYAHERGIVHRDVKPANILMPTPSWPMLSDFGIVKLLTES
                     DNQNLTAQGMIVGTPAYLAPEQVLGMHIDRRTDLYSAGIVLYEMVTGRVPFDTGTPIV
                     VATKQAYEPPPPPLTVNPNLPKAVELVVLRALEKDPANRFQSAADMATALERVVRQLD
                     NARTPQPVPSTPEDGAGEEDLDANY"
BASE COUNT          581 a         1162 c         1242 g          559 t
ORIGIN      
        1 caccagttcg tgctgcgggc agaccccggc cagttcgacc gtgtcgccca ggtcgagccg
       61 ggcgatagtc gcggccagtt cggcctccag cggcccctcg ccgtagatgg cgcagcggaa
      121 gcggtagccc gcctgcttga gctgggcgca ggccgccagc aggtcgggaa agcccttctt
      181 ctcgaccagg cggcccaccg acagcagcag cggctggttc gcggcgggcg cggcctcttt
      241 agaggcagcc ggggccgggc ggaagcgacc cagatcgacg ccgtggtgga tgagccgcac
      301 cttggcctgg agcgcgggcg ggatggtctc ttgcaggtag tcgatattcg cggcgcagca
      361 ggtcacgacg gtgcgcgccc ggcgcacccg gtcgatcagc gcggcgggcg ggatctggta
      421 gaggtcacgc gcgtgggcgg tgaagctata gggcaggccg gtcagcatct gggccagcac
      481 cgcgatcagc gtgggatcgt gggcgaaatg ggcgtgcagg tggccggcgg cgcgcccctg
      541 gcggcgctgc tcgacgcgca gcaaccgcgc caggtagacg gcctgcgcga agcaggcgaa
      601 gcgcgacgcg gcatgatagc ccgtgtcgat gtcgcggtgg gccagcacat agcgcagggc
      661 gcggacgtag cgccggggcg cggccagggc caggcgcagg tgctcggcgg cgatgagcgc
      721 cgcggggcgt tgcgtggccc cctccaggta gtgcaccggc gcccgcacct gggccacctc
      781 cggctgcacg accggttcgc gcgggtcggt gcccgcgaaa atcacgatcc gcacgccccg
      841 ctgttcgagg gccaggatct cgttcaggat aaaggtctgc gagagtcgcg gataactccg
      901 gaggatatag ccgacccagg gtcgcgggcg cgcatccatc gttgtaacct ctccaggatc
      961 gcagcacgac gagtccagtg gcgcgccggc ccggttgccc ggtggtccgc gcgccgcgcc
     1021 agggtttatt tttccgccgc cccgtcgggg cgcaacgcgc cgggcttgga catgatcagc
     1081 gtggcgaatg tgcccgacat ctgccggccc acgtcgtagc cgagcgcggt cagcccgtgg
     1141 gcgaccatca gccggagctg gcggtcctgt tcctcgccct cgggccgggg ccgcagcccg
     1201 cggcgggtgc agagctgctt ggcgatcttc aaacaggtgt agccgtagaa gtagacgaac
     1261 cgggggttgg ccgtccaccc ggtctggcgg gcataggccc gcacgaagac gctctgcaat
     1321 tcggcgcagc ggcgcggcgg catgttcttg cgctgggcca gcagatagag attggcgcag
     1381 aagtgggcca ggtcgaagtt ggggtcgccc aggcgcatct cgtcgaagtc gatgatcttc
     1441 agcccgtcgc cgaccacgat gtggccgtag tggaaatctt tgtggatcgg ggcctcggcg
     1501 gcgaagccca gattgggcag cgcgtcttgc agcaactggg agatgcgcag ggcgtcctcg
     1561 gcctcgtcag gatatttatg cccgatgagc gcggcccagg cgtgcaggtt ggcgatctcg
     1621 gtggcgggct ggaagcggcg ctccagcggc agccggctct ggtgcagcag gcccagccag
     1681 atgccggcca ggtccatata ccgcacagcc tcgtcgtcga ggatgacctc gtccaggaag
     1741 cggccttccg cgggtagata gaccagcatg gacaggtcgg ggatcacgcc cagcggctgc
     1801 gggataccca cgtcggtgcg cccgccgaag acgcccgccc agagcgggcg catcgtcgcc
     1861 tccacgcgct cggcctggtt ggccgccggg tagagcttgc cgaagaccag gcggcgcggc
     1921 ccgccctcgc ggctggtcac ttcgtagtgg atcaggccgc ggttgccctg cttataggcg
     1981 atcagcttgg cgtaaccgac caccggggtg tggcccggcg gcaccagcga ggccagccag
     2041 ggggccaggt ggcgggtcat ggcctccacg ttcagcgcga cctggagcac gggcaggccg
     2101 gtggtgtaga acgccgtcgg gtgcttgtcc atgctggtgg acgtcatcac ccgcgcgatc
     2161 accgtctcct ggatatcgtt gccggctgcg ggggccgggt cgtcggaccg ctccgccata
     2221 ggggtagacc cctttctcgg gtagtgcggg gaaacgaacg gcaagttgca cgcgaagagg
     2281 agcgaaattg ggcgtggcga cggcgtaact taccgatggg cgcggcagta tatcactaat
     2341 gtaacctata tagcaagtgc gctatttgag gttacggatc cgggcagatg agtatgttgt
     2401 attctagcat aagggacccg gcttgacaag atattcacat gtagtataat ttagaaagtc
     2461 cttatttcgg ctcagttgcg gtattttcga ggcatcctgg ccggcgctcc tcttcgctgt
     2521 ccgcctcgtt ctgcccccct cgcccgccgc ccgagccgat ggtggtcgct gctgcaccgg
     2581 acaggcgcga ccgccccgcc gcgcggcccc gcgtataccc gccgcaaggg agagtcgtga
     2641 tggacgacct catcaacacg caactcggac agtaccgcct caccgaagtg atccggcgcg
     2701 ggggtatgtc cacggtctac aaagcctacc aggagtcgct ggaccgctac gtggcggtga
     2761 aggtgctgtt ccacggccag aacccccagt tcgtggcccg cttccagcgc gaagcccgcg
     2821 ccatcgccca cctccagcat cccaatatcc tgcccatttt cgactacggc gagcaggacg
     2881 gcttgctcta cctggtcctg caatatatcg agcaagggat gacgctgggc gacctgctgg
     2941 gacgcccgat ggagccggtc ccggcgctac agttgatggg ccgggtgctg gccgcgctcg
     3001 actacgccca tgagcgcggc atcgtccacc gcgatgtgaa gccggccaac atcttgatgc
     3061 cgacgccaag ctggcccatg ctgtcggatt tcggcatcgt caagctgctg accgagagcg
     3121 acaaccagaa cctgaccgcg cagggcatga tcgtgggcac gcccgcgtac ctggcgccgg
     3181 agcaggtgct gggcatgcac atcgaccggc gcaccgatct ctactcggcg ggcatcgtgc
     3241 tctacgagat ggtcactggc cgcgtgccgt tcgacaccgg cacccccatc gtggtcgcca
     3301 ccaagcaggc ctacgagccg ccgccgccgc cgctgaccgt gaaccccaac ctgcccaagg
     3361 ccgtcgaact cgtcgtcttg cgcgcgctgg agaaggaccc ggccaaccgc ttccagtcgg
     3421 cggccgacat ggccacggcg ctggagcgcg tcgtccggca actcgacaac gcgcgaaccc
     3481 cccagccggt gccgagcacc ccagaggatg gggccggcga ggaggacctg gacgccaatt
     3541 acat
//
LOCUS       DASUYC010000314         4392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_861043,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000314 DASUYC010000000
VERSION     DASUYC010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4392
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861043"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..821
                     /locus_tag="VF276_05100"
     CDS             138..821
                     /locus_tag="VF276_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009850066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6606276.1"
                     /translation="MRVLVVEDEMRLAALVRQALEEESYAVDVAYTGRAALEWLRLVT
                     YDLIVLDLMLPEIDGLEVCRRYRATGGRTPILMLTARDTLDDRVRGLDSGADDYLIKP
                     FGMPELLARLRALARREGPSKTPQLQVGDLILDTVSKRAQRAGHSIDLTATEYALLEL
                     LMRHPGQVLSRDQIIDHVWNADFGAGSKLIDVYIHYLRRKIDNEYPAKLLHTVRGMGY
                     RIGDENAGA"
     gene            862..2214
                     /locus_tag="VF276_05105"
     CDS             862..2214
                     /locus_tag="VF276_05105"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6606277.1"
                     /translation="MRVRLVLWSLLVNALLLIGLGVGVGLVLRQIQYQQMDDRLHVSA
                     AELTRSVEIDDLQVNIRPNELTELADRGVFGWVVDPAGRVLKAFGQATSLPWPGLWAP
                     GSFRDLTLGSTEVRLYQQEPGQAGSRIAGGISLVLGTSREPAERTSRAVLLILSVAIP
                     LGLAVAAAGAMFLAGRALAPISTITAQARRIGRANLSERLALPGPRDEVGQLADTFDD
                     MLDRLQAAFEHERQFLADASHELRTPLGLLRMQIDLAQSRPRDAATMTAMVRAMGADV
                     DRMTRLVEAMLALTRLESAQAAPAPVDLYDVLGGLVSQLQPLALERQITLALDSTTEA
                     GVLGDRDRLVQLFLNLLDNALKYTPPGGQICVRVRRAAASWEIQVADSGPGIPPEHLP
                     HLFDRFYRVDTSRARETGGLGLGLAIAQAIAHQHGGAITVASIPGQGTIFAVTLPSAF
                     "
     gene            complement(2313..2534)
                     /locus_tag="VF276_05110"
     CDS             complement(2313..2534)
                     /locus_tag="VF276_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606278.1"
                     /translation="MSTQNPPPSALRLSKQAEDEEGDESTTTVLWIGGGALLLLVFLA
                     FLCVGGLLLSSVVAGHPITDIFLTPAPAP"
     gene            complement(2622..3416)
                     /locus_tag="VF276_05115"
     CDS             complement(2622..3416)
                     /locus_tag="VF276_05115"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEX6606279.1"
                     /translation="MAERIAGVARVLAVASGKGGVGKTTVTVNLALALAQTGARTGVF
                     DADIYGPNIPLMLGVRRQEEARGWLPVARAGKEPYIPPLDRFGLQVMSIGLLVGEAQA
                     VMPHPLFTGRIVTQTIRDVLWDRLDYLLVDLPPGTGEPQQSLLQAVPVDGAVVVTTPQ
                     DLSLLDAGRSLAMFRQSHVPILGLVENMSYLYCPHCGERVEVFHHSDRPWAVQDTEIE
                     VLGRIPMDLAISRGIAAGHPLLAANPDAAEAAVFRHIAARVREKLV"
     gene            3559..4320
                     /locus_tag="VF276_05120"
     CDS             3559..4320
                     /locus_tag="VF276_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018466086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6606280.1"
                     /translation="MPWDPQRYLQFATQRAAPFDDLAGLIQIRDGLQVVDLGCGTGDL
                     TRRLADLLPESHVVGIDSSPEMLARAQEQARSGLRFQLGDIATVTGDWDLVFSHAALQ
                     WLPDHPALIPRVLGLVRPGGQLVVQMPSNHGHATHRLIADIAAEEPFSSALGGWTRQS
                     PVLPLEQYADLLYSHGGRDLIVFEKIYPHVLDDADALADWTSGTALVPYFERLPDDLR
                     AAFMDRYRARLRALWPQGPVFYGFRRILFAATRAA"
BASE COUNT          681 a         1477 c         1502 g          732 t
ORIGIN      
        1 attacgccgt tttaggccgc ctgaccgcgc gctaagcggc taagtggcga tcgccggatg
       61 caggaatcgc ggcgaagtgg tacgatacgg acatggtccc atgcggtggt gcggagggac
      121 cggcgaggtc gtgggtcatg cgcgtactgg tggtcgaaga tgagatgcgc ctggccgccc
      181 tggtgcgcca ggcgttggaa gaagagtcct acgccgtcga tgtcgcatac accgggcgcg
      241 cggccctgga atggctgcgc ctggtgacat atgatctgat cgttctcgac ctgatgctgc
      301 ccgagatcga cgggctggaa gtgtgccgcc gctatcgcgc cacgggcgga cgcacgccca
      361 tcctcatgct gacggcccgc gataccctgg acgatcgggt gcgcgggctg gacagcggcg
      421 ccgacgatta cctgatcaag cccttcggca tgccggaact gctggcccga ctacgcgcgc
      481 tggcccggcg cgagggtccg agcaagacgc cccagttgca ggtgggcgac ctcatcctgg
      541 acacggtgtc caagcgtgcc cagcgggcgg ggcactccat cgacctgacg gctaccgaat
      601 acgctttgct ggagttgctg atgcggcacc ccggccaggt tctctcccgc gatcagatta
      661 tcgaccatgt gtggaacgcc gacttcggcg ccggctccaa gctgatcgat gtttacatcc
      721 actacctacg gcgcaaaatc gacaacgagt atcccgccaa actcctccac acggtgcgcg
      781 ggatgggcta ccgtatcgga gacgagaatg cgggcgcata gcgggccatg gtggcggaat
      841 tggctcgcgc gactgcaaac gttgcgcgtc cggttggtgt tgtggtcgct gctggtcaac
      901 gccctgctgc tgatcgggtt gggggtgggc gtcgggttgg tgttgcgcca gatccagtat
      961 cagcaaatgg acgaccgcct gcacgtcagc gccgcggaac tgacccgctc ggtggaaatt
     1021 gacgacttgc aggtgaacat caggcccaat gagctaacgg aattggccga ccggggggtg
     1081 tttggctggg tggtcgatcc cgccgggcgg gtgctcaagg cattcgggca ggccacgtcg
     1141 ctgccctggc ccggcctctg ggcgcccggt tctttccgcg atctcacgct cggttccacc
     1201 gaggtccggc tttaccagca ggaacccggc caggcgggta gccgcatcgc cggagggatt
     1261 tcgctcgtgc tggggacctc gcgcgaaccg gcggagcgca cgtcgcgggc ggtgttgctg
     1321 attctgagtg tggcgatccc gctggggctg gctgtggcgg cggcgggggc gatgttcctc
     1381 gccgggcgcg cgctggcgcc catcagcacg attaccgcgc aagcgcggcg catcgggcgc
     1441 gcaaacctga gcgagcggct ggccctgccg ggcccgcgcg acgaagtggg ccagttggcc
     1501 gacaccttcg acgatatgct agaccgcctg caggccgcct tcgagcacga gcgccagttt
     1561 ctggccgacg cctcgcacga gttacgcacg ccgctgggcc tcctgcggat gcagatcgat
     1621 ctggcgcaga gccgcccgcg cgacgcggcc accatgaccg cgatggtgcg ggcgatgggc
     1681 gccgacgtgg accgcatgac ccggctggtt gaagcaatgc tggccctgac ccgcctggag
     1741 agcgcgcagg ccgcgcccgc accggtggat ctgtacgatg tgctgggcgg cctggtgagc
     1801 caactccaac cgctcgcgct ggagcggcag atcacccttg ccctcgacag caccaccgag
     1861 gcgggtgtgc tgggagatcg ggatcggctc gtgcagctat tcctcaacct gctggacaat
     1921 gcgctgaagt acactccacc aggcgggcag atctgcgtcc gggtgcggcg cgcggcggcg
     1981 agctgggaga tccaggtcgc cgatagcggg ccggggatcc ctccggagca tctgccgcac
     2041 ctgttcgacc gcttctaccg ggtcgatacc tcgcgggcgc gcgaaacggg cggtctgggc
     2101 ctcggcctcg ccatcgcgca ggccatcgcc caccagcacg gcggcgccat caccgtcgcg
     2161 agcatccccg gccagggcac tatcttcgcc gtcaccttgc cttcggcgtt ctgaccccgg
     2221 cctcctacgc gggcgtcaaa aaccccgatc cttctcccgc atgtcctgcg gtgatgatcg
     2281 gggtctggcg gccagcggcg gcggatgcgg gatcagggcg cgggggccgg ggtcaggaaa
     2341 atatcggtga tcgggtgccc ggcgacgacc gaactgagta acaggccgcc cacgcacagg
     2401 aaggcgagaa acaccagcag caagagcgcc ccgccgccta tccacaagac ggtagtggtg
     2461 ctctcgtcgc cctcttcatc ctcagcttgc ttcgacaagc gcagcgcgga tgggggtgga
     2521 ttttgcgtgc tcatggatcg aaactcctcc tctgggcaat cgcctgccgc gacgacacgt
     2581 gtggcagcgc gccccattat accgttaacc cgccggcagc gtcaaaccaa tttctcgcgc
     2641 acgcgggcgg cgatgtgccg aaagacggcg gcctccgcgg cgtcggggtt ggcggccagc
     2701 agcgggtgcc ctgcggcgat cccgcggctg atcgccaggt ccatcgggat gcggcccagt
     2761 acctcgatct cggtatcttg cacagcccag ggccggtcgc tgtggtgaaa cacctccacc
     2821 cgttcgccgc agtgcgggca gtacaggtag ctcatgttct cgaccagtcc caggatcggc
     2881 acatggctct ggcggaacat ggcgagcgac cggcccgcgt ccagcagcga caggtcttgc
     2941 ggcgtggtga cgaccacggc gccgtccacc ggcaccgcct ggagcaggct ctgttgcggc
     3001 tccccggtgc cgggcggcag atcgaccagc agatagtcga gccggtccca gagcacgtcg
     3061 cggatggtct gggtcacgat gcgcccggtg aacagcgggt gaggcatgac ggcctgggcc
     3121 tcgcccacca gcagcccgat tgacatcacc tgcaggccga accgatccag cggcgggata
     3181 tacggctctt tgccggcgcg ggccacgggt agccagccgc gcgcttcctc ttgccgccgc
     3241 acgcccagca tcagcgggat gttggggccg tagatatcgg cgtcgaagac gccggtgcgc
     3301 gcgccggtct gggccagggc cagcgccagg ttcacggtca cggtggtttt gcccacgccg
     3361 cccttgccgc tggcaaccgc cagcacccgc gccacgcccg cgatccgctc cgccatgccc
     3421 gctcctccct gtccgccgtt cgtcttctat ataacgtgcc atccggggcg aaacgcgctg
     3481 cccaccggga tgacaagggc gggcgcgcgg agtatgatag gaaggatcac agccactcgc
     3541 ctggaaggag atacacccat gccgtgggac ccccaacgct atctgcaatt cgccacccag
     3601 cgcgcggcgc cgttcgacga cctcgccggc ctgatccaga tccgcgacgg gctacaggtc
     3661 gtggatctgg gctgcggcac gggcgaccta acccgccgcc tggccgatct gctgcccgaa
     3721 agccatgtcg tgggcatcga cagctcgccc gagatgctgg cccgcgcgca ggagcaggcc
     3781 cgatcgggcc tccgtttcca gttgggcgac atcgcgaccg tgaccgggga ttgggacctg
     3841 gtgttctcgc acgccgcgct gcaatggctg cccgaccacc cggcgctgat cccgcgggtg
     3901 ctgggcctgg tgcggcccgg cgggcagttg gtggtgcaga tgccgtccaa ccacggccat
     3961 gccacccacc gcctcatcgc cgacatcgcg gcggaagagc cgttcagcag cgcgctcggc
     4021 ggctggacgc gccaatcgcc ggtgctgccc ctggagcagt atgccgacct gctctacagc
     4081 cacggcgggc gcgatctgat tgtcttcgag aagatctatc cccatgtgct ggacgacgcc
     4141 gatgccctgg ccgattggac ctcgggcacg gcgctggtgc cctatttcga gcggctgccc
     4201 gacgatttgc gggcggcctt catggaccgg taccgggccc ggctgcgtgc gctctggccc
     4261 caggggccgg tgttctacgg cttccggcgc atcttgtttg cggcgacccg cgccgcttag
     4321 ttcgcccgcg tacctgagca ggtaggtgcg gcggctgact ctattatgct ttgtgcattt
     4381 cgtctgctca tc
//
LOCUS       DASUYC010000315         3013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1233404, whole genome shotgun sequence.
ACCESSION   DASUYC010000315 DASUYC010000000
VERSION     DASUYC010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233404"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VF276_05125"
     CDS             complement(<1..630)
                     /locus_tag="VF276_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606281.1"
                     /translation="MVSPHSRPAAAREPESAPPRHAPAPDTPTQAALPSPTGAAIPRL
                     VAHSAIHGRGNGTVRATAMQQMQRTSGNRAVQRLLAGRAAPVAPISGRPTAVSVQRNG
                     TGLAGAGAVGQFATNMKAPMAAWDSYGDDLEKRQRALFEPATKQLEALGAPRITSLLK
                     DDRLGDESTYAAFMPATWMVLINPAGLRANATDEAKARLAGTLYHEIRHA"
     gene            884..2455
                     /locus_tag="VF276_05130"
     CDS             884..2455
                     /locus_tag="VF276_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007503050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6606282.1"
                     /translation="METTTAPGQSRTQQSSNGHGTGIETGQLVKHLIGGQWVESAGGG
                     TFTSLNPATGETLAELSQGTAEDANRAVEAARKAFESWRLTPAPKRGEILFRAGQLLI
                     ERKEEIARLMTQEMGKVLAEARGDVQEAIDMTFYMAGEGRRLAGEVVPSELPNKWAMT
                     TRDPLGVVAAITPWNFPVAIPSWKIMPALVAGNTVVIKPASDTPLCAIALIKVLEAAG
                     VPAGVINLVMGPGSTVGTALLEHPDVALISFTGSTDAGNAVAVEAARRLKRVSLEMGG
                     KNAIIVMDDADIDLALEGILWSAFGTSGQRCTAASRVIVHRAVQKELEDRLVARMEKL
                     RLGNGLESTTDVGPVINKSQQQRIHKMVQEGQKAGTKLVTGGKIADEGDLAHGAFYTP
                     SLFTDAKWDDMVAQEEIFGPVTTLVPVDSLDEAIRVNNHTKYGLSSSIYTANVNRAFQ
                     AMRDITTGIVYINAGTIGAEIQAPFGGTRGTGNGHREAGQAGLDTFTEWKTIYVDYSG
                     KLQRAQIDITENPAP"
     gene            2556..2975
                     /locus_tag="VF276_05135"
     CDS             2556..2975
                     /locus_tag="VF276_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606283.1"
                     /translation="MPYDFTLMRLPRPTQPGLESMDDGPQAIMRGQVLQVLAGMPELQ
                     PREPGLLYVADPPDGPHIEVHIDPRNPPEFVSVEFDRYASQADYAAARAVVDRLCNGL
                     RLTIMDDPDAGPNPTADAEAPTGGGSFMDKIRGLFGR"
BASE COUNT          517 a         1010 c         1045 g          441 t
ORIGIN      
        1 ggcatggcgg atctcatgat aaagcgtccc ggccaggcgg gcttttgctt cgtccgtcgc
       61 gttcgcccgc aagcccgcag ggttgatgag caccatccag gtggcgggca tgaatgccgc
      121 gtaggtcgat tcgtccccaa gccggtcgtc ttttaagagc gaggtgatac gcggcgcgcc
      181 gagggcttcc aactgtttgg tggccggttc aaagagggcg cgctgccgct tctccagatc
      241 gtcgccgtag ctgtcccagg cggccatggg cgccttcata ttggtcgcga actggccgac
      301 cgcgcccgcg ccggccagcc cggtgccgtt gcgctggacg ctgaccgcgg tcgggcgacc
      361 actgatcggc gccacggggg cggcccgccc ggcgagcagc cgttgcaccg cgcgattgcc
      421 ggatgtccgt tgcatctgct gcatcgcggt cgcccgcacg gtgccgttgc cccggccgtg
      481 aatggcggag tgggcaacga gccgcgggat ggcggcgccg gtcgggctgg ggagcgcggc
      541 ctgggtcggg gtgtcggggg cgggagcgtg ccggggcggc gcggattccg gttcccgcgc
      601 tgcggcgggc cggctatgcg gagagaccat gaggtacatc tttctttcgc gtggcgcgaa
      661 aatagggcta ttcggtttta gtgcgtctgc tgccaacagt atagcacact tcggctaccg
      721 gtgtgggtgc aacgccaggc ggcggcccgg tatttgattg acactctccg cggctttgtg
      781 atacactttc ggtagcatgg ccggcgttcg cgcccggcgg cgacataggg cgacccagcg
      841 cccggcaaac acaacgacgt gttgaggaga ggagcaagac agcatggaga cgacaaccgc
      901 gcccggccag agccggacgc agcagagcag caacggccac ggcaccggca tcgagaccgg
      961 ccagttggtc aagcacctca tcggcggcca gtgggtcgaa tcggcggggg gtggcacctt
     1021 caccagtctg aacccggcca ccggcgagac cctggccgaa ctgagccagg gcacggcgga
     1081 agatgccaat cgcgccgtgg aggccgcccg caaggccttc gagagctggc ggctgacgcc
     1141 ggcgcccaag cgcggcgaga tcctgttccg cgccgggcaa ttgctcatcg agcgcaagga
     1201 agagatcgct cgcctgatga cccaggagat gggcaaggtg ctggccgagg cccgcggtga
     1261 cgtgcaggag gccatcgaca tgaccttcta catggccggc gaaggccgcc gcctggccgg
     1321 cgaggtggta ccgagcgaac tgcccaacaa gtgggccatg accacgcgcg acccgctcgg
     1381 cgtggtggcc gcgatcaccc cctggaactt cccggtggcg atccccagtt ggaagatcat
     1441 gccggcgctg gtcgcgggca acacggtggt cattaagccg gccagcgaca cgccgctctg
     1501 cgccatcgcg ctgatcaagg tgctcgaagc cgcaggcgtg cccgccgggg tgatcaacct
     1561 ggtcatgggg ccggggtcca ccgtgggcac cgccctgctg gagcacccgg acgtggccct
     1621 gatctcgttc accggctcga ccgacgcggg caacgccgtc gccgtggaag cggcccggcg
     1681 cctgaagcgc gtctcgctgg agatgggcgg caagaacgcc atcatcgtca tggacgacgc
     1741 cgacatcgac ctggccctgg agggcatcct ctggagcgcc ttcggcacca gcggccagcg
     1801 ctgcaccgcg gccagccgcg tgatcgtcca tcgcgccgtg cagaaggagc tcgaagatcg
     1861 gctggtggcg cgcatggaga agctgcgcct gggcaacggc ctggagtcga ccaccgatgt
     1921 cggcccggtc atcaacaaga gccagcagca gcgcatccat aagatggtgc aggagggcca
     1981 gaaggccggc accaagctcg tcaccggcgg caagatcgcc gacgagggtg acctggcgca
     2041 cggcgccttc tacaccccta gcctctttac cgacgccaaa tgggacgaca tggtggccca
     2101 ggaagagatc ttcggcccgg tcacgacgct cgtcccggtg gacagcctcg acgaggcgat
     2161 ccgcgtgaac aaccacacca agtacggcct gtcgagctcg atctataccg ccaacgtcaa
     2221 ccgcgccttc caggcgatgc gcgacatcac caccggcatt gtctatatca acgccggcac
     2281 catcggcgcc gagatccagg cgcccttcgg cggcacgcgc ggcaccggca acggccaccg
     2341 cgaagccggg caggcgggcc tcgacacctt taccgagtgg aagacgatct acgtggacta
     2401 cagcggcaag ctgcagcgcg cgcagatcga cattaccgag aacccggccc cgtagggggt
     2461 agtggcaaaa cccgcgcgca ttcgtttcaa gttccaggct cggggtagcg caggcgccct
     2521 gccgcctgga acttgaaaca ctttcggagg tccgcatgcc ctatgatttc acgctgatgc
     2581 gcctgccccg gcccacccag cccggcctgg agtcgatgga cgacgggccg caggcgatca
     2641 tgcgcggcca ggtgctccag gtactggccg ggatgcccga gttgcagccc cgcgagccag
     2701 gcctgctcta cgtggccgat ccgccggacg ggccgcatat cgaggtccat atcgacccgc
     2761 gcaacccgcc cgagttcgtg tcggtggagt tcgaccgcta cgccagccag gccgactacg
     2821 cggcggcgcg cgccgtggtg gaccggttgt gcaacggact ccggctgaca atcatggacg
     2881 accccgacgc cggccccaac cccacggcgg acgccgaggc gcccaccggc ggcggcagtt
     2941 ttatggacaa gatccgcggg ctattcgggc gctagcagcg cgctgtaggg gccggtttga
     3001 aaccggcccg ccg
//
LOCUS       DASUYC010000316         2172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1856332, whole genome shotgun sequence.
ACCESSION   DASUYC010000316 DASUYC010000000
VERSION     DASUYC010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1856332"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /gene="bchD"
                     /locus_tag="VF276_05140"
     CDS             complement(<1..1008)
                     /gene="bchD"
                     /locus_tag="VF276_05140"
                     /inference="COORDINATES: protein motif:HMM:NF036831.4"
                     /note="involved in chelation of magnesium into
                     protoporphyrin IX; involved in bacteriochlorophyll
                     biosynthesis; part of a complex with BchI, BchD, and BchH;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase ATPase subunit D"
                     /protein_id="HEX6606284.1"
                     /translation="MTPDLSPATALALLALDPALGGAVFWGADGWALEPWAAALTALR
                     GPEAQVLRLPLDAGPAQILGGLDLAASLRTGRRVARPGLLATHDGGVLWLPDPDLLPA
                     ETACPLTTALRQGAVQLERDGVSARLPARLLLLTTAAPGTPPRYPALLAQAGLWVPAT
                     GALDADPPGDLAAWVAGARTRLPDIALAEHQIAALAAVALRMGVPGLRADYIAARTAR
                     AAAALAGRTTVEDADLELAVALVLAPRATLTPAEASAPPPPPAAEPPAPEPASGDQEP
                     AAPPPEASPAPDPAPGPAAQAERLIAAQAAVLPPNILTTSRPRPPRNARSAVGAGRAA
                     GE"
     gene            complement(1005..2096)
                     /gene="bchI"
                     /locus_tag="VF276_05145"
     CDS             complement(1005..2096)
                     /gene="bchI"
                     /locus_tag="VF276_05145"
                     /EC_number="6.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0010007 - magnesium chelatase complex
                     [Evidence IEA];
                     GO_function: GO:0016851 - magnesium chelatase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase ATPase subunit I"
                     /protein_id="HEX6606285.1"
                     /translation="MDTGIPRPFADSESLPAEPQPISAAAPPPYPFTAIVGQDELKTA
                     LLIAAINPRVGGVLILGQRGTGKSTIVRALAGLLPPVTAVAGCRYGCDPAGPLCDECR
                     ADPARPARRALRPVPVVDLPLGVTADRLTGSLDLEQALGQGVRAYEPGLLARANRGFL
                     YIDEVNLLEDHLVDLLLDVAASGVNIVEREGLSVRHPARFVLAGSANPEEGELRPQLL
                     DRFGLLVRVETITDLDQRVEIVRRREAYEHDPGAFARRYAPAQLALARQVRAAQKALP
                     RVAFPDPLLRDTAALCRDLDVDGHRGEITIVRAARALAALEGRVTATVADVRRVAVPA
                     LRHRLRRGPLDPLDGGERVTEVLDHIFPA"
BASE COUNT          287 a          767 c          866 g          252 t
ORIGIN      
        1 ctcgccggcg gcccggcccg cgccgactgc gctgcgtgcg ttgcggggcg ggcgggggcg
       61 cgaggtcgtc aggatgttgg gcggcaggac cgccgcctgc gcggcgatca gccgctcggc
      121 ctgggccgcg gggccgggcg ccgggtccgg cgcgggcgac gcttccggag gcggggcggc
      181 gggttcctgg tcgccgcttg ccggctcggg ggccggtggt tccgctgccg ggggcggcgg
      241 aggcgcggat gcttcggcgg gggtcaaggt ggctcgtggg gccagcacca gggcgacggc
      301 aagctccaga tcggcgtctt cgaccgtcgt gcgcccggcc agggccgcgg cggcgcgggc
      361 ggtgcgggcc gcgatgtagt cggcgcgcag gccgggcacg cccatccgca acgccacggc
      421 ggccagggcg gcgatctggt gttcggctag cgcgatgtcc ggcaagcggg tccgcgcccc
      481 ggccacccag gcggccaggt caccgggcgg atcggcgtcg agtgcgccgg ttgccgggac
      541 ccagaggccg gcctgggcca ggagagccgg gtagcggggt ggcgtgccgg gcgcggcggt
      601 ggtcagcagc agcaggcggg cgggcaggcg tgcgctcacg ccgtcgcgtt ccaactggac
      661 ggcgccctgg cgcagggccg tggtcagcgg gcaggcggtc tcggcgggca gcagatcggg
      721 atcgggcagc cagaggacgc cgccgtcgtg ggtggcaagc agcccagggc gggcgacacg
      781 ccgcccggtg cgcaggctgg cggcgagatc gagcccgccc aggatctgcg ccgggccggc
      841 gtcaagcggc aaccgcagta cctgtgcttc cggcccgcgc aaggcggtca gcgcggcggc
      901 ccagggttcc agcgcccagc cgtcagcgcc ccagaagacc gcgccgccca gcgccgggtc
      961 gagcgcgagc agcgccagcg cggtggcggg cgacagatcg ggcgtcatgc ggggaagatg
     1021 tgatccagca cctcggtcac gcgctcgcct ccgtcgagtg ggtcgagcgg cccgcggcgc
     1081 aggcgatggc gcagagcggg cacggccacc cgccgcacat cggcgaccgt agccgtgacc
     1141 cggccctcca gcgcggccag ggccctggcc gcccgcacga tggtgatctc gccacgatgg
     1201 ccgtccacgt ccaggtcgcg gcagagcgcc gccgtgtcgc gcagcaacgg gtcggggaag
     1261 gcgacgcggg gcagggcttt ctgggcggcg cggacctggc gggccagggc gagctgcgcg
     1321 ggggcgtagc gccgggcgaa ggcgccggga tcgtgctcat aggcttcgcg gcggcgcacg
     1381 atctccactc gctggtccag gtcggtgatc gtctccacgc gcaccagcag gccgaagcgg
     1441 tcgagcaact gcgggcgcag ttcgccctcc tcggggttgg ccgacccggc cagcacaaag
     1501 cgcgccgggt ggcgcacgct caaaccctca cgctccacga tgttgacccc gctggccgct
     1561 acgtccagca gcagatcgac caggtgatct tccagcagat tcacctcgtc gatatacaga
     1621 aagccgcggt tggcgcgcgc cagcaatccc ggttcatagg cccgcacccc ctggcccagc
     1681 gcctgctcca ggtccaggct gccggtcagc cggtcggcgg tcacgcccag gggcagatcg
     1741 accacgggca ccgggcgcaa agcgcgccgg gccggtcgcg ccgggtcggc ccggcactcg
     1801 tcgcagagcg gcccggcggg atcgcaacca tagcggcacc cggccaccgc cgtcacgggc
     1861 ggcaacagcc cggccagggc gcgcacaatg gtcgatttgc ccgtgccgcg ctggcccagg
     1921 atcaagacgc cgcccacgcg cgggttgatc gccgcgatca gcagggccgt cttcaactca
     1981 tcctggccga cgatggccgt gaacgggtag ggcgggggcg ccgccgcgct gatcggctgc
     2041 ggctcggcgg gaagcgattc gctatctgcg aaggggcggg ggatgccggt gtccatggcc
     2101 tcattgtacc ggcagcgccc ctccggcgca agctaatgat aggcatccca taggccaaac
     2161 tcagccgcgg ta
//
LOCUS       DASUYC010000317         3210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_996122,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000317 DASUYC010000000
VERSION     DASUYC010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996122"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VF276_05150"
     CDS             <1..311
                     /locus_tag="VF276_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017519304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEX6606286.1"
                     /translation="GGTGFSGRDSTPEAVSVLFDKHIEGFGEVFRHYSLAEIGTSTLQ
                     SRALAGLANGTVIFCVPGSTNACRTAWDRIIGEQLDNRHRPCNFVPQLKGAACETSRG
                     "
     gene            304..1560
                     /gene="glp"
                     /locus_tag="VF276_05155"
     CDS             304..1560
                     /gene="glp"
                     /locus_tag="VF276_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016391310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX6606287.1"
                     /translation="MAERGLPAADACYPKGLLGVDDAIARLVALARERRPGVETMALA
                     QAAGRVLAQDLVARRDVPPEANSAMDGIAFRLADLPASGEMRISQRIPAGVAPVALEP
                     GTAARIFTGAILPSGADTVAMQEHCDFGDGRVRLRQCPAPGANVRPAGQDVAAGQVML
                     GAGSRLGAAALGLAAAAGLAALPVYRPLRVAILCSGDELLEPGEPWRSAAIYNSNRPM
                     LAALLRGWDVDVTDLGKVADTPAATTAALARAAATGADVIVSTGGVSVGEEDHVRAAL
                     ERAGALAFWKVAIKPGKPVAVGRLGDSVFLGLPGNPQSVFVTALLLARPFLLAAQGQA
                     AVAPPQFPVAAGFATRKAGDRREYLRVRCETGPGGLRLSPHPQQSSGALLSAAWADGL
                     ALVEAGVRVEEGVALPFLPFAGLLSP"
     gene            1803..2861
                     /locus_tag="VF276_05160"
     CDS             1803..2861
                     /locus_tag="VF276_05160"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX6606288.1"
                     /translation="MNRKLLALAIGTALALPVAAQAAPTVYGKLDLSVEHLDGDVGIA
                     GVAADISDTTLETNKSRIGVRGEEKLNGSLTAVYQVEWGVQADGDGANTTSGVDLGMR
                     DRFLGLRHETLGTLRLGRMNTPLKEAEGKVDVFNNSRLDMENVFTGQNRENNSIAYTS
                     PKIADALTLRFTQITEAGAASGGGSDAGQSMSVVFEQEGLYLAAALDNDVSGDADDGI
                     LNAPAGSFYGATGLELDTIRLVAAVNVGDLRIGGLLQKAEGEGLGAQPEQTGFLLSAA
                     YTAGKWTFKGQYGASEGELGAADLGEINQLALGADYQWTQTFKTYGLLGLFEFDDPAP
                     GNADTDLTLLGVGMALTF"
BASE COUNT          493 a         1125 c         1093 g          499 t
ORIGIN      
        1 ccggcggcac cggcttctcc ggtcgcgact ccacgcccga ggcggtgtcc gtgcttttcg
       61 acaagcacat cgagggcttc ggcgaggtct tccgccacta ttcgctggcc gagatcggca
      121 ccagcacgct gcagtcgcgc gcgctcgccg gcctcgccaa cggcaccgtg atcttctgcg
      181 tgccgggctc caccaatgcc tgccgcaccg cctgggaccg catcatcggc gaacagctcg
      241 acaaccggca ccgtccctgc aatttcgtgc cgcagctcaa gggcgcggcc tgcgagacct
      301 cccgtggctg agcgcgggct ccccgccgcg gacgcctgtt atccgaaggg cctgctcggc
      361 gtcgatgacg cgatcgcgcg gctcgtggcg ctcgcgcgcg agcgccggcc gggcgtggaa
      421 accatggcgc tggcccaggc cgccggccgc gtgctggcgc aggacctggt ggcgcgccgc
      481 gacgtgccgc ccgaggccaa cagcgccatg gacggcatcg cctttcgcct cgccgatctc
      541 ccggcctccg gggagatgcg catcagccag cggatcccgg cgggggtggc gccggtagcg
      601 ctggagcccg gcaccgcggc acgtatcttc accggcgcca tcctgccgtc cggcgccgac
      661 accgtcgcca tgcaggagca ctgcgacttc ggggacggcc gcgtgcgcct gcggcagtgt
      721 ccggcgcccg gcgccaacgt ccgccccgcc gggcaggacg tcgcggccgg gcaggtgatg
      781 ctcggcgcgg gcagccgcct cggcgccgcc gcgctgggcc tggccgccgc cgccgggctg
      841 gcggcgctgc cggtctaccg gccgttgcgc gtcgccatcc tgtgcagcgg cgacgaactg
      901 ctggagccgg gcgagccctg gcgcagcgcc gccatctaca actccaaccg gccgatgctc
      961 gcggcgctgc tgcgcggctg ggacgtcgac gtcaccgacc tgggcaaggt cgcggatacg
     1021 ccggcggcca ccacggcggc gctggcgcgg gcggcggcca ccggcgccga cgtcatcgtg
     1081 tccaccggcg gcgtgtccgt gggcgaggag gatcatgtgc gcgcggcgct ggagcgggcc
     1141 ggcgcgctgg cgttctggaa ggtagccatc aagccgggca agccggtggc cgtcggccgc
     1201 ctgggcgaca gcgtgttcct cggcctgccg ggcaatccgc agtcggtttt cgtcaccgcc
     1261 ctgctgctgg cgcggccctt cctgctggcc gcgcagggcc aggcggcggt ggcgccgccg
     1321 cagttcccgg tggcggcggg ctttgccacg cgcaaggcgg gtgatcgccg cgaatacctg
     1381 cgggtgcgct gcgagacggg tcccggcggg ctgcgcctgt cgccgcaccc gcagcagtcc
     1441 agcggcgcgc tcctgtccgc ggcctgggcc gacggcctgg cgctggtgga ggcgggcgtg
     1501 agggtcgagg agggcgtggc cttgcccttc ctgccgttcg caggattgct ctccccttag
     1561 tgggaggggc gtgccgctgg gcacaaggga gcccctcggc tccctttttt ctggaacaag
     1621 caccacgctt gctgtccacc tcagggcgtc gtcatcaagt tgtcatcaaa tcttcatgtc
     1681 ggctataagt cagggtagcc accgcaccag gcgagtgcat gaacacaggc atgtgccgcc
     1741 ttgcagggca tggcccggtc ggtggcttca taccaagaat gagtatcaag gagatacccc
     1801 gcatgaatcg caaactgctc gctcttgcca ttggcaccgc cctggccctg ccggtcgcgg
     1861 cgcaggccgc tcccaccgtc tacggcaagc tggacctgtc cgtggagcac ctcgacgggg
     1921 atgtcggcat cgccggcgtg gccgctgaca tttccgatac caccctggaa accaacaagt
     1981 cccgcatcgg cgtgcgcggc gaggaaaagc tcaatggctc ccttaccgcc gtctaccagg
     2041 tcgagtgggg cgtacaggcc gatggcgacg gcgccaacac caccagcggc gtcgacctgg
     2101 gcatgcgcga ccgcttcctc ggcctgcgcc acgagaccct cggcaccctc aggctgggtc
     2161 gcatgaacac gccgctgaag gaggcggagg gcaaggtgga cgtgttcaac aacagccgcc
     2221 tcgacatgga gaacgtcttc accggccaga accgtgaaaa caactccatc gcctacacca
     2281 gcccgaagat tgccgatgcc ctgaccctgc gcttcaccca gatcaccgag gccggcgccg
     2341 cctcgggcgg tggcagcgat gccggccagt cgatgtcggt agtgttcgag caggaaggcc
     2401 tctacctggc cgcggccctg gacaatgacg tctcgggcga tgccgacgac ggcatcctga
     2461 acgcgccggc cggctccttc tacggcgcca ccggcctgga gctggacacg atccgcctgg
     2521 tggcggcggt gaacgtcggc gacctgcgca tcggcggcct gctgcagaag gcggagggcg
     2581 agggcctggg cgcccagccc gagcagaccg gcttcctgct gagcgcggcc tatacggccg
     2641 gcaagtggac cttcaagggc cagtacggcg cctccgaggg cgagctgggc gccgccgacc
     2701 tgggcgagat caaccagctc gcgctggggg ccgactacca gtggacccag accttcaaga
     2761 cctacggcct gctgggcctg ttcgagttcg acgatcccgc gccgggcaac gccgacaccg
     2821 acctgaccct gctgggcgtg ggcatggccc tgaccttcta agcggatgcg gcaaggaaaa
     2881 ggcccgccac ccggcgggct ttttcttgcc tggcgaagct aatgccgtat atgaataaaa
     2941 atataagttt atatatttaa attataggtc tgtaatgtcg ctgtaatgac cgggtcactg
     3001 ccctgtcatt tgtccccgaa atacttgcgg ccagttgtgg atcagccatg acgccaactg
     3061 ccaactcaaa gcaagcaaga atttcgagga gaaactgata atgaagcgtt cactgattgc
     3121 cctgggtgtc gcggccgcac tggctcttcc cgtcgccgcc caggcggccc ccaaggttta
     3181 cggcaagctc aacgtgaccg tccagaacgt
//
LOCUS       DASUYC010000318         2131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1228823, whole genome shotgun sequence.
ACCESSION   DASUYC010000318 DASUYC010000000
VERSION     DASUYC010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228823"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1324
                     /locus_tag="VF276_05165"
     CDS             <1..1324
                     /locus_tag="VF276_05165"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX6606289.1"
                     /translation="RQRPPTAILSSFTRPAWAPSALPILDAQRPPGQQSGLTATAAVD
                     GGHDLSRIPIHSPTPAAIQTKRVVSEPGDADEQEADRQADQVMRASRPSAPEAGGPAP
                     AGPHSGEAMALDPPDRQFFESRFHHNFADVRVYAGRDANESARRLQARAYTVGNSIHF
                     APGEYRPGTPAGRHLLAHELAHVLQQRALPGSQPARIQRQPEPAKQAPKAPPAQQKTL
                     KEAGVGLDDPVFEKTAQIIDEVLLRNQRLAPYIGDKLKSGLRIAEKGKFVQELSDSNF
                     ENSYRTAYDQNAAQTVPAHIVGFYDPKKSEVHVRPDARFGTALHEAVHRLASATLYNY
                     YLPAAMKISGNLAEVLKEGLTAFFTDCILKDEELTNYNDAYRDVKAKAAQIMKALGSD
                     GFDLLAKFNFKGGGIVEIGEKLGLSRKQYADLKGAGQEEVLKRMNKLL"
     gene            complement(1352..1762)
                     /locus_tag="VF276_05170"
     CDS             complement(1352..1762)
                     /locus_tag="VF276_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX6606290.1"
                     /translation="MPAPRWLARFNRHVTNRILGGVARYLPGFGVITHTGRKTQRPYR
                     TPVNVFRHPGGYVVALTYGPRADWVRNVLASGGCTLETRGRRVPLTRPRLLHDAERRA
                     VPALVRPFLGLLHVYDFLELERAGTAGRPASPHE"
     gene            complement(1875..>2131)
                     /locus_tag="VF276_05175"
     CDS             complement(1875..>2131)
                     /locus_tag="VF276_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606291.1"
                     /translation="FLYYRSVIEDLIRCGPVPPIKIWMDVGLHEWFLAPNRRMQALLR
                     ERGYDVRYTEQTSGHNYPSWRNVLWRGLEYLYGVSGPEPE"
BASE COUNT          417 a          706 c          676 g          332 t
ORIGIN      
        1 gcggcagcgc ccgccaactg ctatactctc cagttttacg cgccccgcct gggcaccatc
       61 cgccctgccg atcctcgatg cgcagcggcc acccggccag caatccggat taaccgctac
      121 cgctgccgtg gacggtggcc acgatcttag ccgcatccct atccactccc ctaccccggc
      181 ggcgatccag accaaacggg tagtcagcga gccgggagac gccgatgagc aggaggcgga
      241 ccgccaggcg gaccaggtca tgcgcgcgag tagaccgtct gctccggagg ccgggggccc
      301 tgcgccggcc gggccgcatt ccggcgaggc catggcgctc gatccgccgg atcggcagtt
      361 ctttgaatcg cgcttccatc acaatttcgc cgatgtgcgc gtgtatgccg gccgggacgc
      421 caacgagtcg gcgcgcaggc tccaggcgcg ggcgtatacc gtgggtaaca gcatccattt
      481 cgcacccggc gaatatcgcc ccggcacccc ggcgggccgg catctgctgg cccacgaact
      541 ggcgcacgtc cttcagcaac gggcgttgcc ggggtcgcag ccggcgcgca tccagcgcca
      601 gccggaaccc gccaagcaag cgcccaaagc gccccccgcc cagcaaaaaa cgctgaaaga
      661 ggcaggagtc ggcctcgacg acccggtgtt cgagaaaacc gcacaaatca tcgacgaagt
      721 gttgctgcgc aatcaacgac tggcgccata cattggcgat aagctgaagt cgggtttgcg
      781 gatcgccgag aagggaaaat tcgtccagga actgagcgac tccaatttcg agaattccta
      841 ccgcacggca tacgatcaga atgccgcgca aaccgtgccc gcccatatcg tcgggtttta
      901 tgaccccaag aaatccgaag tgcatgtgcg gcctgacgcc cgcttcggca cggcgttgca
      961 tgaagccgtg caccggctgg caagcgcgac cctgtataac tattacctgc ccgcggcgat
     1021 gaaaatctcc ggcaacctgg ccgaggtgtt aaaggaaggc ttaacggcgt tcttcaccga
     1081 ttgcattttg aaggacgagg aattaaccaa ctataacgac gcctatcgcg acgtgaaagc
     1141 gaaggccgcc cagataatga aggcgttggg gtccgatggc ttcgacctgc tcgcgaaatt
     1201 caatttcaaa ggcggcggca tcgtggaaat cggcgaaaaa ctgggcctct cgcgcaaaca
     1261 atacgccgat ttgaagggcg ccggtcagga agaagtgctc aaacggatga acaagttgct
     1321 gtaacgcccg gcgaatcgcg ggcatgccag cctactcgtg tggggacgcg ggccgtccgg
     1381 cagttcccgc ccgttccagt tcgagaaagt cgtagacgtg caacagcccg aggaacgggc
     1441 ggacgagcgc gggcaccgcc cggcgctccg cgtcgtggag cagccggggc cgggtgagcg
     1501 gcacccggcg cccgcgcgtt tcgagggtgc agccgccgct cgccagcaca ttgcgcaccc
     1561 aatcggcccg cgggccgtag gtcagggcga cgacatagcc gccggggtgc cggaacacgt
     1621 tcaccggcgt gcggtacggg cgctgggtct tgcgtcccgt gtgcgtgatg acgccgaaac
     1681 ccggcaggta ccgggccacg ccgcccagga tgcggttcgt gacgtggcga ttgaagcgag
     1741 ccagccagcg gggcgcgggc atcggcagta cctccagtgc gtatggtggg aggcgttcgt
     1801 caggcactcc cccggcgttg cttaagctca ttggcggcgc gcccagcgcg accgatccgc
     1861 aggccggccc ccgtctattc cggctccggg ccgctcaccc cgtacaggta ttcgagcccc
     1921 cgccacagca cattgcgcca ggacggataa ttgtggccgc tggtttgctc ggtatagcgc
     1981 acatcgtagc cacgctcgcg cagtagcgcc tgcatccgcc ggttgggcgc caggaaccac
     2041 tcgtgcaggc ccacgtccat ccagattttg atcggcggca ccggaccaca acggatcaag
     2101 tcctcgatca ccgatcggta gtacaggaag t
//
LOCUS       DASUYC010000319         3430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1626180, whole genome shotgun sequence.
ACCESSION   DASUYC010000319 DASUYC010000000
VERSION     DASUYC010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1626180"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1277
                     /locus_tag="VF276_05180"
     CDS             <1..1277
                     /locus_tag="VF276_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009981758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX6606292.1"
                     /translation="AGEAVGYTNAGTVEFILDSEGNYYFLEMNTRLQVEHPITEETIH
                     VPMQPGAAPASLDLVELQLRVAQGEPLPFTQEAVERRGHAVEVRVYAEDPLKGFLPSI
                     GRLRVFQPPGGPGVRNDVGVEAGDQVSIYYDPMLAKLIAFGPDRATAIRRLSVALAAY
                     EVAGVTTNLGFLFWLAQHPAFREGATDTGFLDRYFDPAAPSGAPPPPEVVLAAGAYDL
                     VCGSASQPAANPWTAGPWRQAGQDVVFRYGTLVPGGAAAGREAAHRVTATFSRGDAPG
                     TWRATAAGWSGLVRIVPNAAGGIQLRWLDGEGPVDLPVQVAAPAPDRLVVIWDARIYA
                     LLRLPPLSTDTLGVAAHLGEDKLEAPMPGKIIQVLAGEGDAVGEGQRLVVMEAMKMEF
                     TIKAPHAGWVTRLPVRVGQLVEAGAILAEVVE"
     gene            1283..2203
                     /locus_tag="VF276_05185"
     CDS             1283..2203
                     /locus_tag="VF276_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX6606293.1"
                     /translation="MANTPLQNLPAAVTVVEVGPRDGLQNEKAPVPTEAKIAFINLLS
                     ASGLPVIEATSFVSPRAVPQLADAGEVFAAIDKMPGVRYPVLVPNTRGMERALAAGVR
                     EVAVFTAASESFTRHNINATIAESLANFAAVIALAREHDVRVRGYVSTAFGCPYEGHV
                     PPEKVRDVTMRLFDLGVAEVSVGDTIGVATPNGILEVMGLLLRDLPADHLALHLHDTR
                     GTALANVLAALQLGITIFDSAAGGLGGCPYAPGASGNLATEDLLYMLDGLGIHTGVDL
                     DKVVAASRYLAGVRGSVPASKYFQAVQARA"
     gene            2317..>3430
                     /locus_tag="VF276_05190"
     CDS             2317..>3430
                     /locus_tag="VF276_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606294.1"
                     /translation="MIRPIRLLLPVVMLLLAVLPWSGASRAASGDRYYPETGFTVPAI
                     FADYWDKHGGLPIFGYPITAAQMENGYLTQYFERNRFEYHPEHAGTPYEVLLGLLGDT
                     LTVGRDFPKADPFPDNPTHRYFAATGHDLQGSFLQYWEQNGGLAQFGYPISDELQETS
                     ATDGKTYTVQYFERNRFEYHPENRPPYDVLLGLLSRDYLAHLGPPSSPPASGPFPGWT
                     PNPPAGYPPAPAGPFLSGPHVGFGITVHMYYQDHKRILDAVQDIGFNWIKQQVQWKDI
                     EGSPGAYAWAELDSIVADTAARRINLMISVVKAPAWANGGGNGFPTDPTYLRNFMHAM
                     AAHYGGQVQAYEIWNEQNLFSESGDLNPARYAELLKA"
BASE COUNT          550 a         1158 c         1181 g          541 t
ORIGIN      
        1 gcgccggcga ggcggtgggc tataccaacg cgggcaccgt ggagtttatc ctggactcgg
       61 agggcaacta ttatttcctg gaaatgaaca cacgcctgca ggtggagcac ccgatcaccg
      121 aggagacgat ccacgtgccg atgcagccgg gcgccgcgcc ggcgtcgctg gatctcgtcg
      181 agttgcaact gcgggtggcg cagggcgaac cgctgccctt cacccaggag gccgtcgagc
      241 gccggggcca cgccgtcgag gtgcgcgtct acgccgagga tccgctgaag ggtttcctgc
      301 cgtccatcgg gcggctgcgc gtgttccagc cgccgggcgg accgggcgtg cgcaacgacg
      361 tgggggtgga ggccggggac caggtcagca tctactacga ccccatgctg gcgaagctga
      421 tcgcgttcgg gccggaccgg gccacggcga tccgccgctt gagcgtggcc ctggcggcct
      481 acgaggtggc cggggtgacg acgaacctgg gcttcctgtt ctggctggcg cagcacccgg
      541 ccttccgcga gggcgccacc gataccggct ttctcgaccg ctatttcgac cccgctgcac
      601 cgtccggcgc gccgcccccg cccgaggtgg tcctggcggc aggcgcgtat gatctggtct
      661 gcgggtcggc gtcccaaccc gcggccaacc cctggacggc cggtccctgg cggcaggccg
      721 ggcaagatgt ggtcttccgc tacggcaccc tggtgccggg cggagcggcg gccggccgcg
      781 aggccgcgca ccgcgtgacc gcgaccttct cccgcgggga tgcgcccggc acctggcgcg
      841 cgaccgcggc gggctggagc ggcctggtgc ggatcgtgcc gaatgccgcc ggcggcatcc
      901 agctgcgatg gctggacggc gaggggccgg tcgatctgcc tgtgcaggtg gcggcgccgg
      961 cgcccgaccg gctggtcgtc atctgggacg cccggatcta cgcgctgttg cggctgccgc
     1021 ccttgagcac agacacgctc ggcgtcgctg cccacctggg cgaagacaaa ctggaggcgc
     1081 cgatgccggg caagatcatc caggtcctgg ccggggaagg ggacgcggtt ggcgaggggc
     1141 agcgcctggt ggtgatggag gcgatgaaga tggagttcac gatcaaggcg ccgcatgccg
     1201 ggtgggtcac gcgactgccg gtgcgcgtgg gccaactggt cgaggccggc gcgatcctgg
     1261 ctgaggtggt ggagtagaga ccatggcaaa tacccccctg caaaacctgc ccgccgccgt
     1321 gaccgtggtc gaagtggggc cgcgcgacgg gttgcaaaat gaaaaggcgc ccgtgcccac
     1381 cgaggcgaaa atcgccttca tcaatctgct ctcggccagc gggctgccgg tgatcgaggc
     1441 gacctcgttc gtcagcccgc gcgccgtgcc gcaactggcc gacgccggag aggtcttcgc
     1501 ggcgatagac aaaatgcccg gcgtgcggta tcctgtgctg gtgccgaaca cgcggggcat
     1561 ggagcgggcg ctggccgccg gggtgcgcga ggtggccgtg ttcacggcgg cctcggagag
     1621 cttcacccgg cacaacatca acgccaccat cgccgagtcg ctggcgaact tcgccgccgt
     1681 gatcgcgctg gcgcgagaac acgacgtgcg ggtgcgcggc tacgtctcga cggccttcgg
     1741 ctgcccctac gaagggcatg tgccgccgga gaaggtgcgc gatgtgacga tgcgcctgtt
     1801 cgacctgggc gtggccgagg tctcggtcgg cgataccatc ggcgtcgcca cgccgaacgg
     1861 catcctggag gtgatgggcc tgctcttgcg cgatctgccg gcggatcacc tcgcgttgca
     1921 tctgcacgac acgcggggca cggccctggc gaacgtgctg gcggcgctgc aactgggtat
     1981 cacgatcttc gactcggcag cgggcgggct tggtgggtgc ccctatgcac ccggcgcgtc
     2041 aggcaacctg gctaccgagg acctgctgta catgctggat ggactgggca ttcataccgg
     2101 cgtggacctg gacaaagtcg ttgccgccag ccgctacctg gcgggcgtgc gcggcagcgt
     2161 cccggccagc aaatactttc aggcggtcca ggctcgcgcc tgagccggtg ttcgccccga
     2221 cctggaacat ccctctgcgt aacgcgtcat gaatacaagc gcctgatgcg gccaaccggt
     2281 tctaattaca tatcccttta gacaaaggac ccacccgtga tccgccccat acgcctgctc
     2341 ctcccggtcg tcatgctgct gctggccgtc ttaccctggt ctggtgcgag tcgcgccgca
     2401 tcgggggatc gctactaccc ggagaccggc ttcaccgtcc ccgccatctt cgccgactac
     2461 tgggataagc acggcgggct gcccatcttc ggctacccga tcacggcggc gcagatggaa
     2521 aacggctacc tcacccagta cttcgagcgc aaccgctttg aataccaccc tgagcacgcg
     2581 ggcacgccct atgaggtcct cctgggcttg ctgggcgata cgctgacggt gggccgggat
     2641 ttcccgaagg ccgatccctt cccggataac ccgacgcacc gctatttcgc agccacgggg
     2701 cacgatctac aggggtcctt tctgcaatac tgggagcaga acggcgggtt ggcccagttc
     2761 ggctacccga tctccgatga gttgcaggaa accagcgcca ccgacggcaa gacgtacacg
     2821 gtgcagtatt tcgagcgcaa ccgtttcgag tatcaccccg aaaaccgccc gccctacgac
     2881 gtgctgctgg gcctgctgag ccgcgactac ctggcgcacc tggggccgcc gagttcgccg
     2941 cccgcctccg gccccttccc cggctggacg ccgaacccgc ccgccgggta tccgcccgcg
     3001 ccggcggggc ccttcctcag cgggccacac gtgggcttcg gcatcaccgt gcatatgtac
     3061 taccaggacc acaagcgcat tctcgacgcg gtgcaggata tcggcttcaa ctggatcaag
     3121 cagcaggtgc aatggaagga catcgaaggg tcgccgggcg cttatgcctg ggccgaactc
     3181 gacagcatcg tggccgacac ggcggcacgc cggatcaacc tgatgatcag cgtggtgaaa
     3241 gcgcccgcgt gggccaacgg cggcgggaac ggcttcccca ccgacccgac ctacctgcgc
     3301 aacttcatgc acgccatggc cgcgcactac ggcggccagg tgcaggccta tgagatctgg
     3361 aacgagcaga acctgttcag cgagagtggg gatctgaacc ccgcgcgcta cgccgaactg
     3421 ttgaaggcag
//
LOCUS       DASUYC010000320         2439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1099761, whole genome shotgun sequence.
ACCESSION   DASUYC010000320 DASUYC010000000
VERSION     DASUYC010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1099761"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VF276_05195"
     CDS             <1..696
                     /locus_tag="VF276_05195"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEX6606295.1"
                     /translation="AVPIWGYLRRGGRCRVCGLALPRWWPIVEAAMALLALLAYISAD
                     GWNARFLLYLFDLAVLLTVLVMDWRRHEIYTVVLLAGALGGLIGGFLLPEIGLGGIAW
                     GALVGGVIMLVMYGLGRVLGRIRYGREGLAWGDVELAVMLGLITGFPGIVTPLFWGPI
                     VGVALFLRRGLGNYFPFAPGLCLVTMMFLLTHTSHEPLWVTLRLPLLGNIFAFIGLII
                     WGVIRAILGIPRG"
     gene            728..1636
                     /locus_tag="VF276_05200"
     CDS             728..1636
                     /locus_tag="VF276_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HEX6606296.1"
                     /translation="MAHVSSPPATARILDGRALAADLRAEAATAIAALKRRHPVLPGL
                     AIVIAGDDPASRGYLRMILKTCATVEVPAQVIDLPGRITRGVLQAEIARLNVLPEVAG
                     VIVQMPLPPPLGPETVSEVLDPAKDVDGIHPENAGRLALSYSGLDYFVPPTPQAGIAL
                     LRRNGISLAGKSALVIGRSAVVGRPLALMLLAANATVTTAHSHTPPAVLRRLLAEADV
                     VASAVGRPNLVRGDMLKPGAVVLDFGVSVVDGVMRGDVDFASAQEVASAITPVPGGIG
                     PVTNLMLVQNTIKAARRMLNDPWPAS"
     gene            1684..>2439
                     /locus_tag="VF276_05205"
     CDS             1684..>2439
                     /locus_tag="VF276_05205"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEX6606297.1"
                     /translation="MPFPTDLEISRAAAMLPITDIAAQMGLDEDDLEPYGRYKAKIKL
                     AALPKLQDRPNARYIDVTAITPTPLGEGKTTTTVGIGEGFKHLGKRSIVAIRQPSMGP
                     IFGIKGGAAGGGYSQIVPMEDFNLHLTGDIHAIGAAHNLCSAFLDASLLHGNKLNIDP
                     LTITWKRVVDMNDRALRQIVVGLGGKENGIPRESGFDITVASEVMAVLALCTSMQDLR
                     ARLGRIVVGRRYDGSLVTTEDLRVAGAMTALLRD"
BASE COUNT          349 a          828 c          839 g          423 t
ORIGIN      
        1 gccgtcccca tctggggcta cctgcggcgc ggggggcgct gccgggtctg cggcctggcg
       61 ctgccgcgct ggtggcccat cgtcgaagcc gccatggccc tgctggcgct gctggcctac
      121 atcagcgcgg acggctggaa cgcgcgcttc ctgctctacc tgttcgacct ggccgtgctg
      181 ctcaccgtgc tggtcatgga ctggcgccgc cacgagatct acaccgtggt gttgctggcc
      241 ggggcgctgg gcgggctgat cggcgggttt ctcctacccg agatcggtct gggcgggatc
      301 gcctggggcg cgctggtcgg cggcgtgatc atgctggtca tgtatggcct gggccgcgtg
      361 ctggggcgca tccgctacgg gcgcgagggc ctggcctggg gcgatgtcga actggccgtg
      421 atgctcggcc tcatcaccgg ctttcccggc atcgtcacgc cgctgttctg gggccccatc
      481 gtgggcgtgg ccctgttctt gcggcgcggg ttggggaact atttcccctt cgcgcccggc
      541 ctctgcctgg tcacgatgat gtttttgctg actcatacca gccacgagcc gctatgggtc
      601 acgttgcggt tgcccctgct gggcaacatc tttgccttta tcgggctgat catctggggt
      661 gtgatccggg ctatcctggg catcccgcgc ggctaggtat agataggaat agagaggcag
      721 gccctgcatg gcccatgtaa gttctccgcc ggccacggcg cgtattctcg acgggcgcgc
      781 cctggccgcc gatctgcgtg ccgaggcggc gacggccatc gccgcgttga agcgtcgcca
      841 ccccgtcttg ccgggcctgg cgattgtgat tgccggcgac gacccggcct cgcgcggcta
      901 tctgcgcatg atcctcaaga cgtgcgcgac ggtcgaggtg cccgcgcagg tgatcgatct
      961 gcctggccgc atcacgcgcg gcgtgttgca ggccgagatc gcccggctga acgtgctgcc
     1021 ggaagtggcc ggggtgatcg tgcagatgcc cctgccgccg ccgctggggc cggagacagt
     1081 cagcgaggtg ctcgacccgg ccaaggacgt ggacggcatc cacccggaaa acgcagggcg
     1141 gctggccctg agctatagcg gcctggatta cttcgtgccg cccacaccgc aggccggcat
     1201 cgccttgttg cgccgcaacg gcatcagcct ggccggcaaa tcggccctgg tcatcgggcg
     1261 ctcggcggtg gtggggcggc ccctggcgct aatgctgctg gccgccaacg ccaccgtcac
     1321 caccgcccat tcgcacacgc cgcctgccgt gctgcgccgc ctgctggccg aggccgatgt
     1381 ggtcgcgagc gccgtgggcc ggcctaacct ggtgcgcggc gacatgctca agccgggcgc
     1441 cgtggtgctc gacttcggcg tcagcgtggt ggacggcgtg atgcgcggcg atgtcgattt
     1501 tgcgagcgcg caggaagtcg cgtcggccat caccccggtg cccggcggca tcggtcccgt
     1561 caccaatctc atgctggtgc aaaatacgat caaggcggcg cggcgcatgt taaacgatcc
     1621 ctggcccgcg tcctgagtgt ggacgcctga cacgttcatt tcaatgaaga aatggagctg
     1681 gtaatgcctt tcccgacgga tctggagatc tcccgcgcgg cggcgatgct gccgatcacc
     1741 gacatcgcgg cccagatggg cctggacgaa gatgacctgg agccctatgg ccgctacaag
     1801 gcgaagatca agctggcggc gctgcccaag ttgcaggatc ggcccaacgc gcgctatatc
     1861 gacgtgaccg ccatcacccc gacgccgctg ggcgagggca agacgaccac cacggtcggc
     1921 atcggcgaag gcttcaagca cctgggcaag cgcagcatcg tggcgatccg ccagccgtcg
     1981 atggggccga tcttcggcat caagggcggc gcggcgggcg gcggctacag ccagatcgtg
     2041 cccatggagg acttcaacct ccacctcacc ggcgatatcc acgccatcgg cgcggcgcac
     2101 aacctctgct cggccttcct cgacgcctcg ctgctgcacg gcaacaagct gaacatcgac
     2161 ccgctgacca tcacctggaa gcgcgtggtc gatatgaacg accgcgccct gcgccagatc
     2221 gtggtcggcc tgggcggcaa ggagaacggc atcccgcgcg agagcggttt cgacatcacc
     2281 gtggcctccg aggtcatggc cgtgctggcc ctctgcacca gcatgcagga tctgcgcgcc
     2341 cgcctgggcc gtatcgtcgt gggccggcgc tacgacggca gcctcgtcac caccgaagac
     2401 ctgcgcgtgg ccggcgccat gaccgcgctg ctgcgtgac
//
LOCUS       DASUYC010000321         4570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_932492,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000321 DASUYC010000000
VERSION     DASUYC010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932492"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VF276_05210"
     CDS             complement(<1..392)
                     /locus_tag="VF276_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606298.1"
                     /translation="MSDQNNNYPHHHHHGEYGQGEGGNQGNPNQDPYSNNAAYSNPTT
                     NFGGGQGYAPQDQENYGGQGYAPQGQGNYGGQSNPAEDQVRYGQGPQRGPGAGAPGST
                     FDLNDPQQRQAALQWLQQFQNQGADSGNT"
     gene            complement(557..1906)
                     /locus_tag="VF276_05215"
     CDS             complement(557..1906)
                     /locus_tag="VF276_05215"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX6606299.1"
                     /translation="MFAPCRKAWSIALLCLLAGSLVLAACDTGTAPAATPTPVAGTQD
                     YGVPKNPKIAALGPVDLHVLFAADYYQVKPVVDVVNAFMQMYPNVHVTLEGTDWNQIP
                     ARVKTEIAGGTMSIDLAHQHAFVMGAEGYAESIDDLWPAIDADKLSPGSIEDTVWRGV
                     HYGVPLDVNSLFTIYRKDLFQKAGLPAPSANWTYEQAREYARKLTQGDVYGTVLGNSA
                     WAMAGHVLANGGSLLSADGKTAGLAAPATVQISQFFSDLINVDHVSPPPPAPGQRFDP
                     VTVFWSGKAAILWTGPFDLGRLRNEAPDSFKAICPNLSCIGTATLPHGMDGTTTGSVQ
                     GGGSLFIPKGARNKEVAFELMRWYLSPVYQLAMVRDQARYPVLKDLYNNPELRDDPLV
                     LPFYEQLKTAHPYTLEANPDADQAWSGAVSAILAGAPASETLEAAQQKAQAALDAHP"
     gene            complement(2219..4015)
                     /gene="ade"
                     /locus_tag="VF276_05220"
     CDS             complement(2219..4015)
                     /gene="ade"
                     /locus_tag="VF276_05220"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HEX6606300.1"
                     /translation="MPPEPVDNAQSAAQAARLARIAARIRVAHGDAPADLVLTGGQVV
                     NVLTGEVYPADVAILTGEIAAVGPPGAYQGREQHDVGGQLLCPALIDGHVHVESSMML
                     PRDFARAVVPHGTLTVIHDPHEIANVCGLDGIRFMLAASAGLPLDVRIMLSSCVPATH
                     METSGARLLAADLAMLAWEDRVLGIAEVMNYPGVIGGTDDLLRKIDLGLAAGRPVDGH
                     APGVRGYDIAAYAASGITSDHESFVLEEAREKLRLGFHVLVREGSTTRNLDALLPLIT
                     PLTSAHCSFCTDDKHPDDIQREGHIDYIVRRAIAGGLDPVLAVQCASYNTARHYGLRD
                     RGAIAPGYRADLLVVDRFPDFNVTGVYRAGALVAAEGRLLQEPPPTEVPPTFTQTMRI
                     DPACVAEDSTYLHVPADGGNRVHVIVMIPHQIVTGRDVATMPVVDGRLEADLSQDVLK
                     VAVWERHKACGNVGVGFVRGFGLQRGAIGSSVAHDSHNIVVVGTNDADMLLAARTVGA
                     MGGGQAAVADGQVLVQVPLEVAGLMSARPYEEMARLAHEEVAAAQALGCTLPNPFIAM
                     SFVALPVIPSLKLTDFGLVDVEQFAVIPLVAE"
     gene            complement(4063..>4570)
                     /locus_tag="VF276_05225"
     CDS             complement(4063..>4570)
                     /locus_tag="VF276_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606301.1"
                     /translation="AWEGRGAGIVLIGEQLLPYQIERETQELDVTPIKRRSAPAAEGA
                     AKPRKPAPDPLQYSAGGQRYFQAPRPATPAGNGVDVPAAETDAPRQIVITLNPLGSEQ
                     EDEERMRRLKAILLRHPGSTGVMLYFPDDPLVGMPTHMPLKRTVDASGAFCDEVTALL
                     GAGCYEFR"
BASE COUNT          694 a         1545 c         1586 g          745 t
ORIGIN      
        1 gtgttaccgc tgtcggcgcc ctggttctgg aactgctgca gccactggag ggcggcttgc
       61 cgctgctgcg ggtcgttcag gtcgaatgtg gaccctggag cgcccgcgcc gggtccccgt
      121 tgtgggccct gcccgtagcg aacctggtcc tccgccgggt tgctctggcc gccgtaattg
      181 ccctggccct gcggcgcgta gccctgtcca ccgtaattct cctgatcctg cggcgcgtag
      241 ccctgcccgc cgccgaagtt ggtggtgggg ttgctgtagg cggcgttgtt gctgtaggga
      301 tcctggttgg ggttcccctg gttgccgcct tcaccctggc cgtactcgcc gtgatggtgg
      361 tggtgcggat agttgttgtt ttgatcagac atagtcttgt gatctcctct cgactaagca
      421 tagaccctgg ccggcggcgg ggcgcgcagg cacacctata ccgcgggcgc cagagccgcg
      481 agtctatcga gggatcagag acggcaagtt atgtgccagc cgggggccgg cgcgccccgg
      541 cggggcgcgc cggggcctaa ggatgcgcgt cgagggcggc ctgggccttc tgttgcgcgg
      601 cctccagcgt ctcgctggcg ggggcgccgg ccaggatcgc gctaacggca ccactccagg
      661 cttgatcagc gtccgggttg gcctccagcg tgtaggggtg cgcggtcttc agttgctcat
      721 agaagggcaa caccagcgga tcgtcgcgca gttccgggtt attgtacaga tccttcagca
      781 cgggatagcg cgcctgatcg cgcaccatgg cgagttggta gacaggcgag aggtaccagc
      841 gcatcaactc gaaggcgacc tctttgttgc gcgcgccctt ggggatgaag aggctgccgc
      901 cgccctgcac cgagccggtc gtcgtgccgt ccatgccgtg gggcagcgtg gccgtgccga
      961 tgcaggacag attggggcag atcgccttga aggaatcggg cgcctcgttg cgcagacggc
     1021 ccaggtcgaa cggccccgtc cagaggatgg ccgccttacc cgaccagaac accgtcaccg
     1081 ggtcgaaccg ctggccgggc gccgggggcg gcgggctaac atggtccacg ttgatcaggt
     1141 cgctgaaaaa ctgcgagatc tggaccgtgg cgggcgcggc aagccccgcg gtcttgccgt
     1201 cggcgctcag caaactgccg ccgttcgcca gcacatggcc ggccatggcc caggcgctgt
     1261 tgccgagcac ggtgccgtag acatcgccct gcgtcagctt gcgggcgtac tcgcgggcct
     1321 gttcataggt ccagttcgcc gacggcgcgg gcaggccggc cttctggaac agatccttgc
     1381 ggtagatggt gaagaggctg ttcacgtcca gcggcacgcc gtagtggacc ccccgccaga
     1441 ccgtatcctc gatggaaccg ggcgacagtt tatcggcgtc gatggccggc cagaggtcgt
     1501 cgatggactc cgcgtagccc tctgcgccca tgacgaaggc gtgctgatgc gccaggtcga
     1561 tgctcatcgt cccgcccgcg atctcggtct tcacgcgcgc cgggatctga ttccagtcgg
     1621 tgccttcgag ggtaacatgg acgtttgggt acatctgcat gaaggcgttg acgacatcga
     1681 ccaccggctt gacctggtag tagtcggcgg caaacagcac atgcaggtca accggcccca
     1741 gcgcggcgat cttgggattc ttgggcaccc cgtagtcctg ggtacccgcc accggcgtcg
     1801 gcgtcgccgc tggggctgtg cccgtatcgc aggccgccag caccaggctg cccgccagca
     1861 agcacagcag ggcgattgac cacgctttgc ggcatggtgc aaacatattc ggctctcctc
     1921 caatgtgcga taggcccggc cacgcgcctg gcggcacgtg tcccctccgc gcctatcgac
     1981 cccaccggcc accagggtgc cggcggttgc gctccccaca gcgcactagc atcgtagcat
     2041 agccggcccg gcccgtcaag cggggccgcc ggttcgcggg cacaactcac tggtacagcc
     2101 ctgttgggtc gcactctctt atatcaacgc acgacggcgc cgaaaggtcg catctaccga
     2161 agacagaatc gcgttccaga ttcccccggt gaatgcagcc gggacgggcc ggcgatggtt
     2221 attccgccac tagcgggatc accgcgaact gctccacgtc aaccaggccg aaatcggtca
     2281 gtttgaggct ggggatcacc ggcagggcga cgaacgacat ggcgatgaac gggttgggca
     2341 gggtgcagcc gagagcctgc gccgccgcga cttcctcgtg cgccaggcgg gccatctcct
     2401 catagggccg cgccgacatc agcccggcca cttcgagcgg cacctgcacg aggacctgcc
     2461 catcggccac cgccgcctgc ccgccgccca tggcgcccac cgtgcgcgcg gccagcagca
     2521 tatcggcgtc gttggtgccc accacgacga tattgtggct gtcgtgggcc accgacgagc
     2581 cgatggcgcc gcgctgcaag ccgaagccgc gcacgaagcc cacgcccacg ttgccgcagg
     2641 ccttgtgccg ctcccacacc gccaccttca gcacatcctg gctcaggtcg gcctccaggc
     2701 gcccgtccac caccggcata gtcgcgacat cgcgcccggt cacgatctga tgcgggatca
     2761 tgacgatgac gtggacccgg ttgccgccgt ccgccgggac atgcaggtag gtcgagtctt
     2821 cggccacaca ggccgggtcg atacgcatcg tctgggtgaa ggtgggcggc acctcggtgg
     2881 gcggcggctc ctgcagcaac cggccctcgg cggcgaccag cgcgcccgcc cgatagacgc
     2941 cggtcacgtt gaagtcgggg aagcggtcca ccaccagcag gtcggcgcgg tagccgggcg
     3001 cgatggcgcc ccggtcgcgc aggccataat ggcgcgccgt gttgtacgag gcacactgca
     3061 cggccagcac cgggtcgagg cccccggcga tggcccggcg cacgatgtag tcgatgtggc
     3121 cttcgcgctg gatgtcgtca gggtgcttgt cgtcggtgca gaacgagcaa tgggccgagg
     3181 tcagcggggt gatcagcggc agcaaggcgt cgaggttgcg cgtggtcgag ccctcgcgga
     3241 ccagcacatg gaagcccagg cgcaacttct cgcgcgcctc ctccagcacg aaggactcgt
     3301 ggtccgaagt gatgccgctg gccgcgtagg cggcgatgtc gtagccgcgc acgccgggcg
     3361 cgtggccgtc caccgggcgc ccggcggcca ggcccaggtc gattttgcgc agcaggtcgt
     3421 cggtgccgcc gatcacgccg ggatagttca tcacttcggc gatgcccagc acgcggtcct
     3481 cccaggccag catcgccagg tcggcggcca gcaggcgcgc cccggacgtt tccatgtggg
     3541 tggcgggcac gcaggacgag agcatgatcc gcacgtcgag cggcagcccc gccgacgccg
     3601 cgagcataaa gcggatgccg tccaggccgc agacgttggc gatctcgtgc gggtcatgaa
     3661 tgacggtcag cgtgccgtgg ggcaccacgg cgcgggcgaa gtcgcgcggc agcatcatcg
     3721 agctttcgac atgcacgtgg ccgtcgatga gcgcggggca gagcagttgc ccgcccacgt
     3781 cgtgctgctc gcgcccctga taagcgccgg gcggtcccac cgccgcgatc tccccggtca
     3841 ggatcgccac gtccgccggg tagacctcac cagtcagcac gttgaccacc tggccgccgg
     3901 taagcaccag gtcagccggc gcgtcgccgt gggccacgcg gatccgcgcc gcgatgcggg
     3961 ccaggcgggc cgcctgggca gcagattgtg cgttatcaac cggttccggc ggcatcggca
     4021 gatcctccta taggcaatgg gccggcagtt gccggcccgc gactaacgga attcgtagca
     4081 cccggcgccc agcaaggcgg tgacctcgtc gcaaaaggcg ccggacgcat ccacggtgcg
     4141 cttcaggggc atgtgcgtgg gcatgccgac cagcggatcg tcggggaagt agagcatcac
     4201 cccggtcgat cccggatggc gcagcaagat tgccttgagc cggcgcatgc gctcctcgtc
     4261 ctcttgctcg ctgcctagcg ggttgagggt gatcacgatc tggcgcggcg cgtccgtctc
     4321 ggcggcgggc acgtcgaccc cattcccggc gggcgtggcg ggccggggcg cctggaagta
     4381 gcgctggccg ccggcgctgt actgcaacgg atcgggcgcc ggcttgcgcg gtttggcggc
     4441 accctcggca gcgggggccg accggcgctt gatcggcgtc acgtcgagtt cctgggtctc
     4501 gcgctcgatc tgataaggca ggagttgctc gccgatcagc acgatgcccg caccgcgtcc
     4561 ctcccaggct
//
LOCUS       DASUYC010000322         2856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_937180,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000322 DASUYC010000000
VERSION     DASUYC010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937180"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VF276_05230"
     CDS             <1..305
                     /locus_tag="VF276_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606302.1"
                     /translation="LTEGAGRALAADLASPGARPRALAWFGGLTGLAVLPANVIAAWL
                     WSSYGPAATFGYAAWLGAAACALLLVWGPWITGLRPYHEPEPAPPVADPAVKRPAS"
     gene            complement(370..1731)
                     /locus_tag="VF276_05235"
     CDS             complement(370..1731)
                     /locus_tag="VF276_05235"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX6606303.1"
                     /translation="MKQRIRGRRSLGAVLPLFFLVAACGSEAAPTATPVAAPPPTATT
                     GGGAAAAPATGAVTFQVFGDPAELSVFQAIVEGYKTANPNVKVTINHIPAQAEYMTRL
                     SSALTAGNAPDVFLLNYRRYGQYADKGVLEPLGAWMDKSATLQPGDYYPESLGAFTYQ
                     GTLQCIPQNVSSLSVYYNKDLFQQYNVPLPAAGWKWPDFLKAAQGLTKDTNGDGVLDI
                     HGLGVEPQFIRVAPFVWQHGGEIVDSAENPTKLTLDEPRAREAVRFFVDLSLTYRVVP
                     TEPETKAEDLESRFMKGKLGMFLGSRADTPTFRTIEGFTWDVAPLPGDQTEATILHSD
                     AYCMAATSKNKEAAWDFIQYAQGQAGQEVAAKLGRTVPSRKSVAASPAFLDPAQAPAN
                     SRMYLDVVPSIRRVPITPSWPAVEKVVNEELERAFYGTAPVDIAIQAAIEKANAELAR
                     DQE"
     gene            complement(1756..>2856)
                     /locus_tag="VF276_05240"
     CDS             complement(1756..>2856)
                     /locus_tag="VF276_05240"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX6606304.1"
                     /translation="KINHIPDQNDYMTRLSAAFTAGTPPDVFLLSYSRYGQFASRGVL
                     EPLGSWLDKSPTLKATDFYTQALEVYTYNGTLQCMPLNISSLTVYYNKDLFQKYNVAL
                     PQPGWKWPEFLKTAQALTKDLDGNGTIDIYGVGLEPQLNRLAPFIWAHGQDLVDDPVH
                     PTKLTLDEPLVREVIARFVDLSMTYRVTPTEAETQAEELETRWLNGKLGMFLGARADT
                     AMFRTVKRFTWDVAPIPADETQASILQSEAYCVPAASKNKAAAWDFIQYAAGTEGQTI
                     VAKLGRAVPTLKSVATSPVFLDPTQLPANNQMYLDAIPTLRHLPITPNWPAVESSTDQ
                     ELERAFYGNVPIDIAIQAAVQKAAVDFARDPK"
BASE COUNT          402 a          906 c         1009 g          539 t
ORIGIN      
        1 cgctgaccga aggcgcgggc cgggccctgg ctgccgatct ggcgtcgccg ggcgcccggc
       61 cccgcgcgct ggcctggttt ggcggcctca ccggcctggc cgtgctgccc gccaacgtga
      121 tcgccgcctg gctctggagc agctatggcc ccgccgccac gttcggctac gcggcctggc
      181 tgggcgcggc ggcctgcgcg ctgctgctgg tctgggggcc ctggatcacc gggctgcgcc
      241 cttaccacga gcccgaaccg gcgccccccg tggccgaccc ggcggtgaag cgccccgctt
      301 cgtaaccgcg ccccagggat gaaaacgggg gcggaccatg gtggcccgcc cccgttgcat
      361 gccgccgcgt tactcctggt ccctggcgag ttcggcgttg gctttttcga tagccgcctg
      421 gatggcgatg tccaccggcg cggtgccgta gaaggcgcgc tccagttctt cgttcaccac
      481 cttttccacg gcgggccagc ttggggtgat cggcacgcgg cggatggatg ggaccacgtc
      541 caggtacatg cggctgttgg ccggcgcctg cgccgggtcg aggaaggcgg gcgaggccgc
      601 cacgctcttg cgtgagggca ctgtgcggcc cagcttcgcg gctacctctt gcccggcttg
      661 gccctgcgcg tactggatga agtcccaggc cgcttccttg ttcttggacg tggcggccat
      721 gcagtaggcg tcgctgtgca ggatcgtcgc ctcggtctgg tcgccgggca agggcgccac
      781 gtcccaggtg aagccttcaa tcgtgcggaa ggtgggcgtg tcggcccggc tgcccaggaa
      841 catgcccagc ttgcccttca tgaagcggct ctccagatcc tcggccttgg tttccggctc
      901 ggtcggcacc acgcgatagg tcaggctgag atcgacgaag aagcgcacag cttcccgcgc
      961 gcgcggctcg tcgagggtta gtttggtcgg gttctcggcg ctgtccacga tctcgccgcc
     1021 gtgctgccag acgaacgggg ccacgcggat gaactgcggc tccacaccca ggccatggat
     1081 atcgagcacg ccgtcgccgt tggtgtcctt ggtcaacccc tgggcggcct tgaggaagtc
     1141 cggccacttc cacccggcgg cgggcagggg cacgttgtac tgctggaaca ggtctttatt
     1201 gtagtagacg ctcaggctcg acacattctg cgggatgcat tgcagcgtgc cctggtaggt
     1261 gaacgcgccg agcgactccg ggtagtagtc gccgggttgc agcgtggcgc tcttgtccat
     1321 ccaggcgccc agcggctcca gcacgccttt gtcggcatac tggccgtagc gccggtagtt
     1381 cagcaggaac acatcgggcg cgttcccggc ggtgagcgcc gacgacagcc gggtcatgta
     1441 ctcggcctgc gccgggatgt ggttgatggt cactttgacg ttcggattgg ccgtcttata
     1501 gccctcgacg atggcctgga agacggacag ctccgccggg tcgccgaaga cctggaaggt
     1561 gacggcgccc gtggccgggg ccgccgcagc gcctccgccg gtggtggccg tgggcggcgg
     1621 cgcggcgacg ggcgtggcgg tcggggcggc ctcgctgcca cacgcggcga ccaggaagaa
     1681 gagcggcagc acagcgccca ggctgcgccg cccgcggata cgttgcttca cttatagaga
     1741 tcctttcgcg gttggctact tcggatccct ggcgaaatcc actgcggctt tctgcaccgc
     1801 cgcctgaata gcgatgtcga tcggcacgtt gccgtagaag gcgcgttcca gctcctggtc
     1861 cgtcgaactc tcgaccgcgg gccagttggg cgtgatcggc agatggcgga gagtcgggat
     1921 ggcatccagg tacatctggt tgttcgccgg gagctgggtc gggtccagga acacgggcga
     1981 ggtggcgacc gacttgagcg tcggcaccgc gcggcccagt ttggcgacaa tcgtctggcc
     2041 ttccgtgccc gcggcgtact ggatgaagtc ccaggccgct gccttattct tcgatgccgc
     2101 aggtacgcag tacgcttcac tttgcaggat cgacgcctgg gtttcgtcgg cggggatggg
     2161 cgccacgtcc caggtgaagc gcttgacggt gcggaacatg gccgtgtcag cccgcgcccc
     2221 caggaacatg cccagcttgc cgttgagcca gcgggtttcc agttcttcgg cctgggtctc
     2281 ggcctcggtc ggcgtgacgc gataggtcat gctgagatcg acgaaacggg cgatcacttc
     2341 gcgcaccagt ggttcgtcca gggtcagttt ggttgggtgt accgggtcgt ccacgagatc
     2401 ctggccgtgc gcccagatga acggggcgag ccggttcagt tgcggttcga ggccgacgcc
     2461 gtagatgtcg atggtgccgt tgccgtccag gtccttggtc agggcctggg ccgtcttgag
     2521 gaactccggc catttccagc cgggctgcgg caaggccaca ttgtatttct ggaacaggtc
     2581 tttattgtag taaacggtca ggctggagat gttcagcggc atgcattgca gcgtgccgtt
     2641 ataggtgtag acctccagcg cctgggtgta gaagtcggtg gccttcagcg tcgggctctt
     2701 gtccagccag gagccgagcg gctccagcac gccccgcgag gcgaactggc cgtagcggct
     2761 atagctgagc aggaacacat cgggcggggt acccgccgtg aacgccgccg acagccgggt
     2821 catatagtca ttctggtcgg gaatgtggtt gatctt
//
LOCUS       DASUYC010000323         2958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_44054,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000323 DASUYC010000000
VERSION     DASUYC010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44054"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2276
                     /locus_tag="VF276_05245"
     CDS             <1..2276
                     /locus_tag="VF276_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX6606305.1"
                     /translation="LDEPVVPQAEDALHQGLLNPPLLAALPVSGESGSPVGDLAWSPD
                     GASILTASTDGQTRVWDAAGHQQRFALAGASAGGWAAWSPDGRQICAPDAQGNPRIYD
                     ATTGQERLALAGGDNSVQSVLWSPDGTRILAVGQEGATTIWDAASGRTLVSIPGLNGV
                     RQSSAGGVVPTDAPPSAAWSPDGTRIVLIRADGIAGIWDAATGQERGALAGLMTSVAW
                     SPDGRSILTGGEDGTARIWDAAGGQERAVLRGHTGLVTRAVWSPDGLAIVTTSTDRTA
                     KIWDVGSSLPRATLTGHTDSVTDAAWSPDGQEVLTAGEDQTARVWDARSGLPLYTLAG
                     HNSAVRRIAWSPDGRHILTGAEQETSVRLWQATRGYERAGLQGTPGTITQFTWSPDGN
                     SVLTINDTGEAKVWDTSTGQARFALNPQGPTINYALWSHDGLYIATTADNATATIWDA
                     ARGTVVRDLSGPAEIAPGVAWSPDGKYIAALTGDRQVQVWDAAGGQQRVAVAATAAWA
                     ARPVWSPDSRSLLVASDDGTSAVWSIPDGRKRFMLPSPQPLDPGAGHNPNYAVGMDWS
                     PDGRLLATSTSDGTTQIWDATTGQERFKLDISVTTENGVVWSPDSRYLLTINDDNTVS
                     LWNAADGKEAVTLAGHTDAVLSAAWSGDGRRILTTSGRADGSARVWDAQTGQVRFILP
                     GYTGRVTAAAWSPDSGRIFSAGADGTTRQYMVGIGDVLALAQSRVTRTLTPQERFTYF
                     LEPLPTPARNATPSATP"
     gene            2426..2647
                     /locus_tag="VF276_05250"
     CDS             2426..2647
                     /locus_tag="VF276_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606306.1"
                     /translation="MPATNWEYKIVLLTSLGEYTEAADNHAGTGHINDLGALGWELAQ
                     VLNWDAEAAQGWVVFRREVAPASGDLSPA"
     gene            complement(2651..>2958)
                     /locus_tag="VF276_05255"
     CDS             complement(2651..>2958)
                     /locus_tag="VF276_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEX6606307.1"
                     /translation="VLSFAFQQDAPIVDTNVDRLLSRLFDLHAGRPSAAVRDKRLWAL
                     AAALIPPGHAYLLNQALMDFGAQVCTPYKPVCLFCPLRAYCQWWNPRPDGGVTYTES"
BASE COUNT          523 a         1017 c         1038 g          380 t
ORIGIN      
        1 gcctggacga accggtcgtg ccgcaggccg aagatgcgct gcatcagggc ttgttgaacc
       61 cgcccttgct ggccgcctta cctgtgtcgg gcgagagcgg cagccccgtg ggcgatctcg
      121 cctggagccc ggacggcgcg agcatcctca ccgccagcac cgacgggcag acacgggtct
      181 gggatgctgc cggccatcag cagcgattcg cgctggccgg cgcttcggcg ggcggctggg
      241 cagcttggag ccccgacggc cggcagatct gcgcgcccga cgcccagggc aacccgcgca
      301 tctacgacgc gacgaccggc caggagcgcc tcgcgctggc cggcggcgac aactcggtgc
      361 aaagcgtgct ctggagtccc gacggcaccc gcatcctggc ggtcggccag gaaggggcga
      421 cgaccatctg ggacgcggcg agcggccgaa ccctggtgag catacccggt cttaacggag
      481 tgcggcaaag cagcgcgggc ggggtggtgc cgaccgatgc gccgccgagt gccgcctgga
      541 gccccgacgg cacccgcatc gtgctcattc gcgccgacgg catcgcgggc atctgggatg
      601 cggcgacggg ccaggaacgc ggcgcgctgg ccgggctgat gaccagcgta gcctggagcc
      661 ccgacggccg gagcatcctc accggcggcg aggacggcac cgcccgaatc tgggatgccg
      721 ccgggggcca ggagcgtgcc gtcttgcgcg gccataccgg cctggtgacc cgtgccgtct
      781 ggagccccga cggcctcgcg attgtcacca cgagcaccga ccgcaccgcg aaaatctggg
      841 atgtgggcag cagcctgccg cgcgccacgc taaccggcca taccgacagc gtaaccgacg
      901 cggcctggag ccccgacggc caggaggtgc tgacggccgg cgaggaccag acggcccgcg
      961 tctgggatgc ccgcagcggc ctgccactct acaccctggc aggccacaac agcgcggtgc
     1021 gccggatcgc gtggagtccc gacgggcgcc acattctgac cggcgccgaa caggagacga
     1081 gcgtcaggct ctggcaagct actcgcggct acgagcgggc cggcctgcaa gggacgccag
     1141 gaacgatcac ccagttcaca tggagtcccg atgggaatag cgtgctaacc atcaacgaca
     1201 caggggaagc caaagtctgg gatacgagca ccgggcaggc gcgctttgcg ctcaatccgc
     1261 agggcccgac gatcaattat gccctgtgga gccacgacgg cctgtatatc gccaccactg
     1321 ccgataacgc caccgcaaca atctgggatg cggccagggg aaccgtggtg cgcgacctga
     1381 gcgggccggc ggagatcgcc cctggcgtgg cctggagtcc ggacggcaaa tacattgcgg
     1441 cgctcacggg agatcgtcag gtgcaggtct gggatgccgc gggcgggcag caacgggtcg
     1501 cggtcgcggc gaccgcggcc tgggccgcgc ggcccgtctg gagtcccgac agccggagcc
     1561 tgctggtcgc cagcgacgac gggacatcag ccgtgtggag catccccgac gggcgcaagc
     1621 gcttcatgct gcccagcccc caaccgctag atcccggcgc cggccataat ccgaactacg
     1681 cggtcggtat ggattggagc ccggatggcc ggttgctcgc cacatccaca tccgacggca
     1741 ccacgcagat ctgggacgcg accaccggcc aggaacgctt caaactggac atcagcgtga
     1801 ccacggagaa cggcgtggtc tggagtcccg atagccgcta tctgctgacc atcaacgacg
     1861 ataatacagt cagcctctgg aacgccgccg acggcaagga ggccgtaacc ctggccgggc
     1921 acaccgatgc ggtgctgagc gccgcctgga gcggcgacgg ccggcgcatc ctgaccacca
     1981 gcggccgggc cgacgggtcc gcccgcgtct gggatgcaca gacagggcag gttcgcttta
     2041 tcctccccgg ctataccggc cgtgtcaccg ccgcggcgtg gagccccgac agcgggcgta
     2101 tcttcagtgc gggcgccgac ggcaccaccc gccagtatat ggtcggcatc ggcgatgtgc
     2161 tcgcgctggc ccagagccgg gtcacgcgca ccctcacgcc gcaagagcgc tttacctact
     2221 tcctcgaacc gctgccgacc ccggcccgga acgcaacacc gtctgcgacg ccctgacccg
     2281 ccggtggcgg cgccgtgccc gcgcaggccg gtggattacc ccggaccggg caagcaactt
     2341 gctctagcgg cggcaggcac ggcgctctac cacggcacaa ccgcaacgaa cacccgcgat
     2401 aggccatgca gccaggggag aaacaatgcc ggcaaccaac tgggaataca agatcgtctt
     2461 gctgacaagc ctgggcgagt acacggaagc ggcggacaac cacgccggca cgggccacat
     2521 caacgacctc ggtgcgctgg gctgggaatt ggcccaggtg ctcaactggg atgctgaggc
     2581 cgcacagggc tgggtggtgt tccgccggga ggtagccccg gcttccggcg atctgtcgcc
     2641 ggcatagggt tcaggactcg gtataagtca cgccgccgtc agggcggggg ttccaccact
     2701 ggcagtaggc gcgcagcggg cagaacaggc agaccggctt atagggcgtg cagacctgcg
     2761 cgccgaagtc catcagcgct tggttgagca ggtaggcgtg gccgggcggg atgagcgccg
     2821 cggccagtgc ccagagccgc ttgtcgcgca cggcagcgct gggccgcccg gcgtgcagat
     2881 cgaacaggcg gctcagcaat cggtccacgt tggtatcgac aatcggcgcg tcttgctgaa
     2941 aggcgaagct gagcacgg
//
LOCUS       DASUYC010000324         3199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1255188, whole genome shotgun sequence.
ACCESSION   DASUYC010000324 DASUYC010000000
VERSION     DASUYC010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1255188"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VF276_05260"
     CDS             complement(<1..233)
                     /locus_tag="VF276_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606308.1"
                     /translation="MRRLSTFGTLALLILVLLGTVACGGEVPAGPAQPRPMPPAPQGA
                     PKGAPPPMAPAAAPTTTTAAAAGGTVEHLELAP"
     gene            509..1468
                     /locus_tag="VF276_05265"
     CDS             509..1468
                     /locus_tag="VF276_05265"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEX6606309.1"
                     /translation="MGKFSAAMRGLLMLGVALALAGCGGESAAPLPGAAAPAPPQTAV
                     PANPTPATNSAYLVGAAALIPALDDPALRVVDMGSGDEYAAGHIPGAVHIDWSELQVT
                     DTRAPSIAAWQDTVAKLLGQRGISDQNRVVIYDHGTLYGARLWWVLDQLGHAEKQVLD
                     GGFAAWQAAGGPVSQEAATPPAVTYTAHPQPAVLATEAQVQAALGTGVQLVDARNPDE
                     YSGQNTSGTARGGHIPGAVNVPFTTTADGATPRLFKSPDELRALFGAQGVTPDKPVIT
                     YCSTGVRGAVDYFALRLAGFPDVRVYTGSWAEWGNDPAAPLAK"
     gene            complement(1481..1999)
                     /locus_tag="VF276_05270"
     CDS             complement(1481..1999)
                     /locus_tag="VF276_05270"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEX6606310.1"
                     /translation="MASRTTPGKIAPQILEMPAQKMAVVYTRGDPNSAGPDMMPALYG
                     AVYNLKFALKKQGRDFKVAKLRARWLDAHLVPRDQWQGVWALPIPDDTTELVQKKPGI
                     EVKIETWEYGTVAQILHRGPFSTEGPTVARLHAFIEETGYEIAGPHEEEYLTTLDAKN
                     QKTLIRYPVRKK"
     gene            complement(2040..2600)
                     /locus_tag="VF276_05275"
     CDS             complement(2040..2600)
                     /locus_tag="VF276_05275"
                     /inference="COORDINATES: protein motif:HMM:NF025658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
                     /protein_id="HEX6606311.1"
                     /translation="MDGEGTDDLVARMTAFLQQPAHPALTVEILQATPDADLEQVVLD
                     YIFHLIDRSGEDAAVVVPALPEGLQMVFATWLVESEVSNGGFPQYFWNPASEFAVEAL
                     YGYRMLGAQRHAATLEAALATYIRERPQLERFRGGGTPDDFAAWAAYSALGTLDWQFW
                     SLATAEDPHALRIHYIRTHLDEFVAE"
     gene            complement(2786..>3199)
                     /locus_tag="VF276_05280"
     CDS             complement(2786..>3199)
                     /locus_tag="VF276_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606312.1"
                     /translation="LAVKIEPPQLMLGGKLHVHMVVQNTGQVPLRTQGPDSGYTYTTN
                     DSYSSIEGGRYTDQAGLWRVGLDWEGNAGGAPYRYPFRWGFGHTLAPGETATIDGYVT
                     ILKHEQTMWFYAGVLQEGVRIALDGLGRTPISVSW"
BASE COUNT          487 a         1079 c         1106 g          527 t
ORIGIN      
        1 tccggcgcaa gctccagatg ttcaaccgtt ccgcccgctg cggcggcagt agtggtggtc
       61 ggcgccgcgg cgggcgccat gggcggtggt gctcctttag gtgccccttg cggggcaggc
      121 ggcatgggcc gcggctgcgc gggaccggcg ggcacttcgc caccacaggc gacggtgccc
      181 agcagcacga ggatgagtaa agcaagcgtg ccgaaagtcg atagccggcg catgggtggt
      241 tcctttctac cgtgtagcgg cacgcgcagg gcgcgcacgc ctcgtcgcca ggccccaccg
      301 ggtgctggcg cgtgtgcggt cccgcgagcc gcgctcgcga gggtccctcc gatagacaag
      361 gatcctgggc cggccccgca tccttcttgc ttctattgac gcacaccgcc ccgcgccggt
      421 tcccgccccg cgccacgagt ttggccggag atcgggcgcc cgccgggatg cgtgtactgg
      481 acaacagcgc gaaggaggca gagggacgat gggcaaattt tcggcggcga tgcgtggact
      541 gctgatgctg ggcgtggccc tggcgctggc cgggtgcggc ggcgaaagcg cggcgccgct
      601 acccggcgcc gccgcccccg ccccaccgca gaccgcagtg cccgccaatc ccaccccggc
      661 gaccaacagc gcctaccttg tcggcgccgc cgccctgatc ccggcgctcg acgaccccgc
      721 gctgcgcgtg gtggacatgg gcagcgggga tgagtacgcc gcgggtcaca tccccggcgc
      781 ggtgcatatc gactggagcg agctccaggt gacggacacc cgcgccccct cgattgccgc
      841 ctggcaggac acggttgcta aattgctggg ccagcggggg atcagcgacc agaaccgcgt
      901 ggttatttac gaccatggca cgctctacgg ggcgcggctc tggtgggtac tcgaccagtt
      961 gggtcacgcg gaaaagcagg tgctcgacgg cggcttcgcg gcctggcagg cggcgggcgg
     1021 gccggtatcg caagaggcag cgacgccgcc tgccgtgacc tacacggcgc acccgcaacc
     1081 ggcggtgctg gcaaccgagg cccaggtgca ggccgcgctg ggcaccggcg tgcaactggt
     1141 ggacgcccgt aaccccgacg aatacagcgg ccagaacacc agcggcaccg cgcggggcgg
     1201 gcacatcccc ggcgcggtca acgtgccttt caccaccacg gcggacggcg ccacgccgcg
     1261 cctgttcaag agtcctgacg aactacgcgc cctgttcggc gcgcaggggg tgacgccgga
     1321 caagccggtc atcacctact gctcgacggg cgtgcgcggc gccgtggact acttcgcgct
     1381 gcgactggcc gggttccccg atgtgcgggt ctacaccggc tcgtgggccg agtggggcaa
     1441 cgacccggcg gcgcccctgg cgaaatagcg ctgggcggcc ctactttttg cgcaccgggt
     1501 agcggatcag ggtcttctgg ttcttggcgt caagcgtagt caggtattct tcctcgtgtg
     1561 gtccggcaat ttcatagccg gtttcttcga tgaaggcatg gagtcgggcg acagtcgggc
     1621 cttccgtgct gaatggcccc cggtggagga tctgggccac cgtgccgtat tcccaggtct
     1681 cgatcttgac ctcaatgccc ggtttcttct gcaccagttc ggtcgtgtcg tcggggatgg
     1741 gcaaggccca gacgccctgc cactggtctc tgggcaccag gtgggcatcg agccaccgtg
     1801 cccgcagctt cgccaccttg aagtcccggc cctgtttctt tagcgcgaac ttcaagttat
     1861 acacagcacc atacaaagcg ggcatcatat cgggaccggc tgaattcgga tcgccccgcg
     1921 tatataccac cgccatcttt tgggcgggca tctccagaat ctggggcgcg atcttaccgg
     1981 gtgtcgtgcg cgaggccatg cgttcctccg tgatattggg atggggtagg cgggccgggc
     2041 tactcggcca cgaattcgtc caggtgcgtg cggatgtagt ggatgcgcaa ggcgtggggg
     2101 tcttccgccg tggcgagcga ccagaactgc cagtcgagcg tgccgagcgc gctgtaggcc
     2161 gcccaggcgg cgaaatcgtc gggggtgccg ccgccgcgga agcgttccaa ctgcgggcgc
     2221 tcgcggatat aggtcgccag ggcggcctcc agggtcgccg cgtggcgctg ggcgccgagc
     2281 atacggtagc cgtagagcgc ctccacggcg aactcgctcg ccgggttcca gaagtactgc
     2341 gggaagccgc cgttgctcac ctcgctctcg accagccagg tggcgaacac catctgcaag
     2401 ccctcgggca gcgcgggcac aaccactgcc gcgtcttcgc cactgcggtc gatcaggtgg
     2461 aagatgtagt cgagcaccac ctgttccaga tcggcatcgg gggtcgcctg gaggatctcc
     2521 acagtcagcg ccgggtgcgc gggctgctgg agaaacgcgg tcatgcgcgc gaccagatcg
     2581 tcagtgccct caccgtccat cgcgctgctg tctcccttcc tgtcgtgccc atcatccgct
     2641 atctctatat cgccacgggg tgggaaatag cggaagcgcg ggcggcgcga gccggtttca
     2701 aaccggcccc tacatcgcgc aatcggagcg agcggtaggc gggtgctaat cggcccccca
     2761 ccgcgccgga tcggcagggc tggctctacc agcttacgct aatcggcgtg cggcccaggc
     2821 cgtccagggc gatgcgcacg ccctcttgca gcacgcccgc atagaaccac atcgtctgct
     2881 cgtgcttgag gatggtcacg tagccgtcga tggtcgccgt ctcgccgggg gccagggtgt
     2941 gaccgaagcc ccagcggaag gggtagcggt agggcgcgcc gcccgcgttg ccttcccagt
     3001 ccaaccccac ccgccagagg cccgcctgat cggtgtagcg cccgccctcg atgctcgaat
     3061 acgagtcgtt ggtggtgtag gtgtagccgg agtccgggcc ttgcgtgcgc aacggcacct
     3121 ggccggtgtt ctgcacgacc atatgcacgt gcagcttgcc gcccagcatc agttgcggcg
     3181 gctcgatctt caccgccag
//
LOCUS       DASUYC010000325         2403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1184888, whole genome shotgun sequence.
ACCESSION   DASUYC010000325 DASUYC010000000
VERSION     DASUYC010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184888"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VF276_05285"
     CDS             <1..491
                     /locus_tag="VF276_05285"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX6606313.1"
                     /translation="QLLPALVGIASGGDGCADWAELQVNLRLPPDLPPAAATVWLHEQ
                     AAACRMTVLGGTPAWAGPRTTPLHRALARAIRAQGATPRFLLKTGTADLNLVAPAWGC
                     PALAYGPGDAALDHTPDEHIVLAEYLRGIAVLEQTLRLCAQDAAPFILTAETQSPQSA
                     LR"
     gene            786..1370
                     /locus_tag="VF276_05290"
     CDS             786..1370
                     /locus_tag="VF276_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6606314.1"
                     /translation="MAQTRIIIVDDDSLIRMDLRDLLQRMGYLVVGEAGDAQSGINTA
                     RSLRPDLVIMDVRMAGDKDGIDAAATLTAERLAPVLLLTAFSDMDLVQRATEAGVGGY
                     LLKPFSEGELRPAIEVTLARFREFQELSREAQDLRDQLDTRKLVERAKGVLMNQLHLS
                     EEEAFSRIQKASMNTRKPMKAIAEAILLSKQISS"
     gene            2198..>2403
                     /locus_tag="VF276_05295"
     CDS             2198..>2403
                     /locus_tag="VF276_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606315.1"
                     /translation="MMPNIYNMEQQMRDEVKQRWAITNQLPAEASPVRIARLLRALAV
                     EEPAVWHHGQRVAWFAIRIGGGLE"
BASE COUNT          410 a          862 c          757 g          374 t
ORIGIN      
        1 atcaactgct gcccgcgctg gtgggcatcg ccagcggcgg cgacggctgc gccgactggg
       61 ccgagctgca ggtcaacctg cgcctgccgc ccgatctgcc gcccgccgcc gcgaccgtct
      121 ggctgcatga gcaggcggcc gcgtgccgca tgacggtcct gggcggcacc ccggcctggg
      181 ccgggccgcg caccacgccg ctgcaccggg cgctggcgcg agccatccgc gcgcagggcg
      241 ccacgccgcg cttcctgctc aagaccggca ccgccgacct caacctggtg gccccggcct
      301 ggggctgccc cgcgctggcc tacgggccgg gcgacgccgc cctggaccac acccccgacg
      361 agcatatcgt cctggccgaa tacctgcgcg ggatcgccgt gctggaacag actctgcgcc
      421 tttgcgcgca ggatgccgcg ccgttcatcc tcaccgcaga gacgcagagt ccgcagagtg
      481 cattaagatg agtgcttatt acaataagcg tatcctatcc gaatctccgc ggcccctgcg
      541 tctctgcggt gggactcccg gttagcggca aatgttggcc gatagtaaca ttgacatcgg
      601 cgcgaccatc gtgtatattc cacgtcacag agcttgcatg caatgatgcc cgcagtcgcc
      661 tcagcggtga ggagtggctg ggggctgttt gtttcctggg acctgctcca acgccgcagc
      721 gcgggccggg agaaaccgac caaccgccat cgaccatggc cggcttgaaa gcaggagtga
      781 accctatggc ccagactcga atcatcatcg tagacgacga ctcgcttatt cgcatggacc
      841 tgcgcgatct gctgcagcgc atgggctatc tcgtggtcgg cgaggccggc gacgcgcaga
      901 gcggcatcaa cacggcgcgc tccctgcgcc ccgatctcgt gatcatggac gtgcgcatgg
      961 ccggcgataa agacggcatc gacgccgccg ccaccctgac cgccgagcgc ctggcgcccg
     1021 tgctgctgct gaccgccttc agcgacatgg acctggtcca gcgcgccacc gaggccggcg
     1081 tgggcggcta tctgctcaag cccttcagcg agggcgaact gcgcccggcc atcgaggtca
     1141 cgctggcccg cttccgcgag ttccaggaac tgagccgcga ggcacaagac ctgcgcgacc
     1201 agctcgatac gcgcaagctg gtcgagcggg ccaagggcgt gctgatgaac cagttgcacc
     1261 tcagcgagga agaagccttc agccgcatcc agaaggccag catgaacacg cgcaagccga
     1321 tgaaggccat cgccgaagcg atcctgctga gcaagcagat cagcagctag cgccgcccgc
     1381 cgctccccac cggcccggcc ccaccccgcg cgacgcgcgc caacaggggc cgggctgttg
     1441 gtgtgcccgc cgtgcgcccg gcccgcagcg tgggctcatc ccccatccca ccgcgacgag
     1501 agtcttcgct ccgcgtgttg cagctcggcg accggaccaa cctctccccc agcccctccc
     1561 cgccgcgggg aggggagcta ctttccctca taggagcggt tccctccttc ccgccgcggg
     1621 gaggggcgcc gctttctctc tcacgggcga atcccttctt cccgccgcgg gaaggggcgc
     1681 cgctttctct ctcacgagcg aatcccttct tccccgccgc ggggaggggc gtcattttac
     1741 ctcgcagaag cgattccccc ttcccgcggc gggaaggggg gcaggggggt taggttggcc
     1801 gggcgccagg atgccgcccg gcgcgcgaaa accgtgaccc gcaccgccgt atgctcaccg
     1861 ccgctgtcat gcttcgctgc gctcagcatc tcggctcctg tcccatgcgc agctacgccg
     1921 cgcggcgtgg gccaatggac atatgcccta atgtgccccg atccccgtat ccccggcggc
     1981 aaattaccca ttagtaaggc acaacggagc agattcctgt ccgctttttt gtcagctttt
     2041 tgtcgagatc aaaccggcaa gatctgtgct ctaatagagg catcaggaac cgataaggat
     2101 cggcctgaac catcccggat cgagccaata accggcgagc cgcgccacga ggcggcccgc
     2161 cccgaacccg ccgcaagcga aggatgatcg tagagctatg atgcccaaca tctacaacat
     2221 ggaacaacaa atgcgcgatg aggtgaaaca gcgctgggcc atcaccaacc agttgccggc
     2281 ggaagcctcg cccgtgcgca tcgcgcggct gctgcgcgcg ctggcggtgg aggaaccggc
     2341 cgtctggcac cacggccagc gcgtggcctg gttcgccatc cgcatcggcg gcggattgga
     2401 gat
//
LOCUS       DASUYC010000326         2185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_987870,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000326 DASUYC010000000
VERSION     DASUYC010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987870"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1365)
                     /locus_tag="VF276_05300"
     CDS             complement(<1..1365)
                     /locus_tag="VF276_05300"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX6606316.1"
                     /translation="MWHTYLQPTTLEETLRLLQEYAGQARVVAGGTDLIVEVERGIRP
                     TATVIDISRLPDLKYVRAEGGQIRLGALATHNDVLASDACAAGALPLAQACLEVGAPQ
                     IRTRGTVAGNVITGSPANDTITPLMALGAEVVLLSQAGERVVPLAEFYTGVRRTVIQP
                     QELLREIRFPALRADQRGLFIKLGLRRAQAIAVINVAFVVTLDGDTVREARITMGCLA
                     PTIVHARQAEVFLAGKQLTPDVCGEAGRLACADVRPIGDLRGSANYRLATLAALVADG
                     LGRIAAGTQAQGFPERRVLLDTGRPGARAGAPFAGTIETTINGQRYSLAEAQDKTLLN
                     ALRQDAGLTGSKEGCAEGECGACTVWIDGQAAMSCLVPAAQAHNAGITTIEGLAQDDR
                     LHPLQQAFIDHAAVQCGFCIPGMLMAGAKLLEERPQPSMDEVQSALSGNICRCTGYRK
                     IFDAI"
     gene            complement(1613..>2185)
                     /locus_tag="VF276_05305"
     CDS             complement(1613..>2185)
                     /locus_tag="VF276_05305"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein"
                     /protein_id="HEX6606317.1"
                     /translation="KSTLLRAIGTNIVLAQAGGPVCATAMRLPPVTLWTSMRIQDNLE
                     QGVSYFMAELRRLKQVVDAARTTHTAGSPRLFYLLDEILQGTNTAERQIAARRIILYL
                     VGQGALGTVSTHDLTLADVPEVAAAAQPIHFVETVHSDGAGPAMTFDYKIRPGIATST
                     NALRLMEIVGLHLTGGPTSPPTPPRRGEGS"
BASE COUNT          301 a          744 c          777 g          363 t
ORIGIN      
        1 gatcgcgtcg aaaatcttgc gatagccggt gcagcgacag atgttgccgc tgagcgcgct
       61 ctgcacctcg tccatggacg gctgcggccg ctcttcgagc agcttcgccc cggccatcag
      121 catgccgggg atgcagaagc cgcactgcac ggcggcgtgg tcgatgaacg cctgctgcaa
      181 ggggtggagc ctgtcgtcct gggccagccc ctcgatggtg gtgatgccgg cgttgtgcgc
      241 ctgcgccgcg ggcaccaggc aggacatggc cgcctggccg tcgatccaga cggtgcaagc
      301 gccgcactcg ccctcggcgc agccttcctt gctgccggta agcccggcgt cctggcgcag
      361 ggcgttgagc agcgtcttgt cctgggcctc ggccaggctg tagcgctgcc cgttgatcgt
      421 cgtctcgatg gtgcccgcga acggcgcgcc cgcgcgggcg ccgggccggc ccgtatcgag
      481 caggacgcgg cgctccggga agccctgcgc ctgcgtgccc gccgcgatgc gccccaaccc
      541 gtccgcgacg agggcggcca gggtcgccaa ccggtagttg gccgagccgc gcaaatcgcc
      601 gatgggtcgc acgtcggcgc aggccagccg tccggcctcc ccgcacacgt ccggcgtgag
      661 ttgcttgccc gccaggaaca cctcggcctg ccgggcatgg acgatggtcg gcgccaggca
      721 gcccatcgtg atgcgcgctt cgcgcacggt gtcgccgtcg agcgtgacga cgaaggccac
      781 gttgatcacg gcgatggcct gggcgcggcg tagccccagc ttgatgaaca gcccccgctg
      841 atcggcgcgc aaggccggga agcggatctc gcgcaataat tcctgcggct ggatcactgt
      901 gcgccgcacg ccggtgtaga actcggccag cggcaccacg cgctcgcccg cctggctgag
      961 cagcaccacc tcggcgccga gtgccatcag cggcgtgatc gtgtcgttgg ccggggagcc
     1021 ggtgatcacg ttcccggcca cggtgccgcg ggtgcggatc tgcggggcgc ccacttcgag
     1081 gcacgcctgg gccagcggca gcgcgcccgc cgcgcaggcg tctgacgcca gcacgtcgtt
     1141 atgcgtcgcc agcgcgccca ggcggatctg ccccccttcg gcgcgcacat atttcaggtc
     1201 gggtagccgg ctgatgtcga tgacggtcgc cgtgggccgg atgccgcgct cgacttccac
     1261 gatcaggtcg gtgccgcccg ccaccacgcg agcctgcccg gcgtattcct gcaacagccg
     1321 cagggtttcc tccagcgtgg tcggttggag ataggtgtgc cacatcgcat gttccccctg
     1381 cctgggcgcc ggtgcgccag gctgtgattt ttggttaacg ccgatcttag cagatattgg
     1441 ccgggaatac aatgctgcgt cgcggtgggg gcaacctcac ccccggcccc tctccgccgc
     1501 ggagagggga gaacgtgtta ctgctttggc cggctaattt ccgggaagtt tgaggtcaac
     1561 tgccggtctg atccattcag ccacttctcg cggacagcgc aaaccgttcg tctcaactcc
     1621 cctccccgcg gcggggaggg gttgggggtg aggttggccc gccggtcagg tgcaggccga
     1681 cgatctccat caggcgcagc gcgttggtcg aggtggcgat gccgggccgg atcttgtagt
     1741 cgaaggtcat ggccgggccg gcgccgtcgc tgtgcaccgt ctccacgaag tggatcggct
     1801 gggcggcggc ggctacttcg ggcacatcgg ccagcgtcag gtcgtgcgtg gagacggtgc
     1861 cgagcgcgcc ctggcccacc aggtacagga tgatgcggcg cgcggcgatc tgccgctcgg
     1921 cggtattggt gccctgcaag atctcgtcga gcaggtagaa caggcgcgga ctaccggcgg
     1981 tatgggtggt gcgcgccgcg tccaccactt gcttgagccg ccgcagttcg gccatgaagt
     2041 acgacacgcc ctgttccagg ttgtcctgga tacgcatgct ggtccagagc gtcaccggcg
     2101 gcaggcgcat ggccgtcgcg cagaccgggc cgcccgcctg ggccagcacg atgttggtgc
     2161 cgatggcgcg cagcagcgtg ctctt
//
LOCUS       DASUYC010000327         4350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_282850,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000327 DASUYC010000000
VERSION     DASUYC010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282850"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..916)
                     /gene="aroE"
                     /locus_tag="VF276_05310"
     CDS             complement(71..916)
                     /gene="aroE"
                     /locus_tag="VF276_05310"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX6606318.1"
                     /translation="MRVGLIGHPLGHSLSPPMQDAAFAALGLPYRYELIDIPESALPD
                     QVAALRGGDWLGCNVTVPYKTAIAPLLDELHGDAAALGAVNTVRVEAGRLAGYNTDVA
                     GFAADLAAHLPAGATPGRAVVLGAGGSARAVVWALVQQGWEVVVVARRAVQAAQLRDS
                     LPAGGAIIPAALEPPVVAGWLAGARMLVNCTPLGMWPRTADDPLPPDVPLAADLFVYD
                     LVYRPAQTRLLARAAAAGCRTAGGLGMLVGQGAAAFTLWTGYPAPVAIMRAAAEQALA
                     AQHTQ"
     gene            complement(901..2280)
                     /gene="aroA"
                     /locus_tag="VF276_05315"
     CDS             complement(901..2280)
                     /gene="aroA"
                     /locus_tag="VF276_05315"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019122438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX6606319.1"
                     /translation="MSGPLTDLLPPMPTAQEPDTGPGGTAGVLRVHGRAGRLSGRVTI
                     PGDKSISHRAALFGALAAGTTRIRGFLPANDCLSTLQIVRALGVPVTYSREEVVVEGR
                     GPDRVAEAERPLFCGGSGTTMRLLSGILAGLPFYSILDGNAQLRRRPMGRVAEPLRRM
                     GATILGRQGGKLAPLSIQGGGLHGIEYATPVPSAQIKSALLLAGLFADGTTTVREASP
                     SRDHTERMLAAMGVPVDTRTPGAVSVTRSTLQPLTIMVPGDISSAAFLLCAGAMVPGT
                     EIRLDGVGVNPTRTGVIDVLRAMGADIQEAAPRQEHGEPVADLIVSARPLRGTRIAGA
                     LIPRLIDELPVLAVAATQAEGVTEVRDAAELRVKETDRIATVAEELGKLGAHIEPLPD
                     GFRVHGPTPLHGAAVGSHGDHRLAMALAVAGLVAEGETVVQGSACIDDSYPGFAAALA
                     ALGAECASD"
     gene            complement(2286..3326)
                     /gene="aroF"
                     /locus_tag="VF276_05320"
     CDS             complement(2286..3326)
                     /gene="aroF"
                     /locus_tag="VF276_05320"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX6606320.1"
                     /translation="MLVVMRVGVDDAEVEHVMQRIREFGNTPVLAAGTERVCIGMLGD
                     ERGINPDEILMMSGVDRVVPILAPYKLASRDFQPTDTIVQVGDQAIGSKDVVFMAGPC
                     SVESRAQTMELAAMLHELGVRIFRGGAFKPRTSPYAFQGMGESGLQILADVREKYGMA
                     IVTEVMGTEEVGLVAQYADMLQIGTRNMQNYRLLEAVGQQPKPVLLKRGMSSTLEELL
                     LAAEYILSQGNRNVVVCERGIRTFEKATRYTFDVNAIPMLKLLTHLPVVADPSHGTGR
                     WDLVVPISRAAVAAGADGLIVEVHQRPEEALSDGAQSLRPDRCAALIEELRRVSRAIG
                     RDVAQVPVAAGG"
     gene            complement(3367..3936)
                     /locus_tag="VF276_05325"
     CDS             complement(3367..3936)
                     /locus_tag="VF276_05325"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015148567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX6606321.1"
                     /translation="MILLIDNYDSFTYNLYQYLGELGAEVQVARNDAVCAEEVQATPP
                     AGLIVSPGPGTPDEAGVSLAAIEALAGQRPVLGVCLGHQCIGQLFGGRIVRAPEPVHG
                     KTSAIYHNGQGLFAGMPNPFQATRYHSLVIDRATCPPDLEITAWTEDGLIMGVRHRRL
                     PIEGVQFHPESIMTEGGKRLLQNYLDMLG"
     gene            complement(3933..>4350)
                     /locus_tag="VF276_05330"
     CDS             complement(3933..>4350)
                     /locus_tag="VF276_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEX6606322.1"
                     /translation="SVRVPILFEIEHYSHVMHLVSHVAGDLRAGLDAVDALRACFPAG
                     TLSGAPKVRAMQIISELEADARGPYGGAVGYLSASGNMDMAITIRTIMLRNGVATVQA
                     GAGIVADSVPATERQESENKARALMVALDLAERGAR"
BASE COUNT          626 a         1590 c         1499 g          635 t
ORIGIN      
        1 caatggccga gactcttcgc tccgctcaga gtgacaaggt tgcgctcaga gtgataatgg
       61 gcgggccagt ctattgagtg tgctgggcgg ccagcgcctg ctccgccgcc gcacgcatga
      121 tcgctaccgg ggcgggatag ccggtccaga gcgtgaaagc cgccgcgccc tgccccacca
      181 gcatgcccag ccccccggcg gtgcggcagc cggccgccgc ggcgcgggcc agcagccggg
      241 tctgcgccgg gcggtagacc aggtcataga caaacagatc ggcggccagc ggcacgtccg
      301 gcggcagcgg gtcgtccgcg gtgcgcggcc acatgccgag cggcgtgcag ttgaccagca
      361 tccgcgcccc ggccagccac cccgcgacga ccggcggctc cagcgccgcc gggatgatcg
      421 ccccgcccgc gggaagcgag tcgcgcaatt gcgcggcctg gaccgcgcgc cgcgccacca
      481 cgacgacttc ccagccctgc tgcacgagcg cccagacgac cgctcgcgcc gatccgcccg
      541 cgcccagcac cacggcccgg cccggcgtgg cccccgccgg tagatgcgcc gccaggtcgg
      601 cggcgaaccc ggcgacatcc gtattgtacc ccgccagccg cccggcttcc acccgcacag
      661 tattgaccgc tccgagcgcc gccgcgtcgc cgtgcaactc gtccagcagg ggcgcgatgg
      721 cggtcttgta gggcacggtc acgttgcagc cgagccagtc gccgccccgc aacgcggcga
      781 cctggtcggg cagcgcgctt tcggggatgt cgatcagctc atagcggtag ggcaggccca
      841 gcgcggcgaa cgccgcgtcc tgcatcggcg gcgagaggct gtggcccagc gggtggccga
      901 tcagtccgac gcgcattccg cccccagcgc ggccagtgcc gcggcgaagc ccggatagct
      961 gtcgtcgatg caggccgagc cctggaccac cgtctcgccc tcggccacca gcccggccac
     1021 ggccagggcc atcgccagcc ggtggtcgcc gtggctgccg acggcggcgc cgtgtagcgg
     1081 cgtcgggccg tggacgcgga agccgtcggg cagcggctcg atgtgcgcgc ccagcttgcc
     1141 caactcctcg gccaccgtgg cgatgcggtc ggtctccttg acgcgcaact cggcggcgtc
     1201 gcgcacctcg gtcacgccct cggcctgggt cgccgcgacg gccagcaccg gcaactcgtc
     1261 gatcagccgc gggatcagcg ccccggcgat gcgcgtgccc cgtagcgggc gcgcgctcac
     1321 gatcaggtcc gccaccggct cgccgtgctc ctggcgcggc gcagcttcct ggatgtcggc
     1381 gcccatggcc cgcagcacgt cgatcacgcc ggtgcgagtc gggttcaccc ctaccccgtc
     1441 gagccggatc tccgtgccgg gaaccatcgc cccggcgcag aggagaaaag ctgcggagga
     1501 tatgtcgccc ggaaccatga tggtcagcgg ttgcagcgtg gatcgcgtga cgctgaccgc
     1561 gccgggggtg cgggtatcga ccggcacgcc catcgccgcc agcatccgtt cggtgtgatc
     1621 gcggctgggg ctggcctcgc gcaccgtggt cgtgccgtcc gcgaacagcc cggccagcag
     1681 cagcgccgac ttgatctgcg cgctgggcac cggggtggca tactcgatgc cgtgcagccc
     1741 gccgccctgg atgctcagcg gcgccagctt gccgccctgg cggcccagga tcgtcgcgcc
     1801 catgcggcgc agcggctcgg ccacgcggcc catgggccgg cggcgcaact gggcgttgcc
     1861 gtcgaggatg ctgtagaacg gcagcccggc caggatgccc gacagcaggc gcatggtggt
     1921 gcccgacccg ccgcagaaca gcgggcgctc cgcctcggcc actctatcgg ggccgcgccc
     1981 ctccacgacc acttcttcgc ggctgtacgt gacgggcaca cccagcgcgc gcacgatctg
     2041 caaggtcgag aggcagtcgt tggccgggag gaagccgcgg atgcgtgtgg tgcccgcggc
     2101 gagcgcgccg aacagcgcgg cgcgatgcga gatcgacttg tcgccgggga tggtcacgcg
     2161 cccggatagg cgcccggcac gaccatgcac ccgcagcacc ccggcggtgc cacccggccc
     2221 ggtgtccggc tcctgggcgg tgggcatcgg cggcaggaga tcggtaagcg gtccgctcat
     2281 cggcctcacc ctcccgccgc gacgggcacc tgggccacgt cgcggccaat cgcccggctc
     2341 acccggcgta attcctcgat cagcgccgcg cagcggtcgg ggcgcaacga ctgggcgccg
     2401 tccgacaggg cctcttcggg ccgctggtgg acctcgacga tcagcccgtc agcacccgca
     2461 gccaccgccg cccgcgagat cggcaccacc aggtcccagc gcccggtgcc gtggctgggg
     2521 tcggcgacga ccggcaggtg ggtcagcagc ttcagcatgg ggatggcgtt cacgtcgaag
     2581 gtgtagcggg tggccttctc gaaggtgcgg atgccgcgct cgcagaccac cacgttgcgg
     2641 ttgccctggc tgaggatgta ttcggcggcc agcagcagtt cttcgagcgt gctgctcatg
     2701 ccgcgcttga gcagcaccgg cttgggttgc tggcccaccg cttcgagcag gcggtagttc
     2761 tgcatgttgc gcgtgccgat ttgcagcata tcggcgtact gcgcgaccag gcccacctcc
     2821 tcggtgccca tcacctccgt gacaatcgcc ataccgtact tctcgcgcac gtcggccagg
     2881 atctgcaggc cactttcgcc catgccctgg aaggcgtagg gcgaagtgcg cggcttgaag
     2941 gcgccgccgc ggaagatgcg cacgcccagt tcgtgcagca tcgccgccag ttccatcgtc
     3001 tgggcgcggc tctcgaccga acatggcccg gccatgaaca ccacgtcctt gctgccgatg
     3061 gcctggtcgc ccacctgcac aatcgtgtcg gtgggctgga agtcgcgact cgccagcttg
     3121 tagggcgcga ggatcggcac gacccggtcc accccgctca tcatcaggat ctcgtcgggg
     3181 ttgatgcccc gctcgtcgcc tagcatgccg atgcagaccc gctcggtgcc cgccgcaagg
     3241 acaggcgtgt taccgaattc gcggatgcgt tgcatcacat gttccacttc ggcgtcatcc
     3301 acgccgactc gcatcaccac cagcatggtt tccctctttt ccgggcgcgc acgcgccgcc
     3361 cactgatcag cccagcatat ccaggtagtt ttgcaacagc cgcttgccgc cctcggtcat
     3421 gatcgactcc gggtggaatt gtacgccttc gatgggcaac cggcggtggc gcacgcccat
     3481 gatgagaccg tcttcggtcc aggcggtgat ttcgagatcg ggcgggcacg tggcccggtc
     3541 gatgaccagg gaatggtagc gggtagcctg gaacggattg ggcatcccgg caaacaggcc
     3601 ctggccgttg tggtagatgg ccgaggtctt gccgtgcacg ggctcggggg cgcggacgat
     3661 gcgcccgccg aacagttgcc cgatgcactg gtggcccagg cagacgccga gcaccggccg
     3721 ctgcccggcc agcgcctcga tggcggccag cgacaccccg gcctcgtcgg gcgtgcccgg
     3781 ccccggcgag acgatcaacc cggcaggcgg cgttgcctgc acctcctcgg cgcagacggc
     3841 gtcgttacgg gccacctgga cctcggcgcc cagttcgccc aggtattgat agagattgta
     3901 ggtaaacgag tcgtagttat cgatcaggag gatcatcgcg cacctcgctc ggccaggtcg
     3961 agggccacca tcagcgcccg cgccttgttc tcggattcct gccgctccgt cgcgggcacg
     4021 ctgtcggcca cgatgcccgc gcccgcctgc acggtcgcca cgccgttgcg cagcatgatc
     4081 gtgcggatcg tgatcgccat gtccatattg ccgctcgcgc tcaggtagcc cacggccccg
     4141 ccgtaggggc cgcgggcgtc agcctccagt tcggagatga tttgcatcgc gcggaccttg
     4201 ggcgcgccgg agagcgtacc cgccgggaag caggcgcgca gggcatccac cgcgtccagc
     4261 cccgcccgca ggtcgcccgc cacatgcgac accaggtgca tgacgtggga gtagtgctcg
     4321 atctcgaaga ggatcggcac gcgcacgctg
//
LOCUS       DASUYC010000328         3469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1357488, whole genome shotgun sequence.
ACCESSION   DASUYC010000328 DASUYC010000000
VERSION     DASUYC010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1357488"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(251..1252)
                     /locus_tag="VF276_05335"
     CDS             complement(251..1252)
                     /locus_tag="VF276_05335"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6606323.1"
                     /translation="MQSNEPTPAAAIYRPLRAGDEAAVLALWAETFEEDPQRLRHAVL
                     DNPGTRRDQTRVAVAADGTLLSAMHYHRRELRDLDGAPQPVGGLTVVATRPDVRRQGH
                     AGRLLEQTIAAMRADGCAWSLLFTDINPYYERYGWRTHATRYRQGLLAAEQPTPAGAY
                     TTQGYDPRHAPGGWAPLAAVYAAYNARRPLTTVRDLPYWERYAPVKFCAPNARVWVTV
                     PASEAGTVAGYVLAHFTEETFVVLEIGVRADAAEAIPALLAAVVDEARARGIGMGRVY
                     IPREPAIDAALAGIYQQVHEDYHHVLMGLPLAPGWDGARIAAIFAAPGAIAWPADDF"
     gene            1457..1897
                     /locus_tag="VF276_05340"
     CDS             1457..1897
                     /locus_tag="VF276_05340"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEX6606324.1"
                     /translation="MSPLGMYRFYHRLYRRGIPKLPFWLYRLHRMIYACEIHPAAELG
                     PGCQMPHSVGIVIGPEVRTGRNCFIAQGVTLGYRYPGPRTPVPGDGNPTLGDGVVVGA
                     GAKVFGPVTIGDRAVIGANSVVTHAVPAGYLAVGVPAEIRPRRR"
     gene            2152..2673
                     /locus_tag="VF276_05345"
     CDS             2152..2673
                     /locus_tag="VF276_05345"
                     /inference="COORDINATES: protein motif:HMM:NF020908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HEX6606325.1"
                     /translation="MDSVSEHEDKFRAMAERRIREALESGELKVEGGPDKKLDLEENP
                     FVPEEWRLAFRVLKSGNYRPDWIDLANDIENDLAAWRTAADRHFAFLRRRLDDLTADA
                     GGLRRMREEVAALKQRHMRATAYHAHMLDEINQKIHRYNATVPATSLMRPTLARDDEM
                     RRFADRLPAYLNY"
     gene            2738..>3469
                     /locus_tag="VF276_05350"
     CDS             2738..>3469
                     /locus_tag="VF276_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX6606326.1"
                     /translation="MQESASPAASTLSAAPARTPALGPDSVTRQVLPNGLVVLVYPNP
                     AIPTVSARLSMRAGALFDPPGKSGVAGLTAGAMRRGTRRHTFSDLNERTDERGMSLGL
                     DAGFHLLEIGGRSLREDAPFLMETLAEVLREPSFPSDEFERLRSQLITGLMEQEDDTR
                     SVADRDFRRAAYPDGHPYGREAGGTLESVPTITRQDLEDHYRTYVRPDTGILVIVGDI
                     SPEAGLDLARQALGDWQVDGVAPALV"
BASE COUNT          496 a         1226 c         1230 g          517 t
ORIGIN      
        1 ctacttggcc ctggaaagcg acctgaaccg caccgtcgtg ctggccgcca tcctggtgct
       61 gttcagcttc acgctgttgc tagccttcaa gctgctcggc gggcgccgcc tcgacccgct
      121 gcccgactaa ccgtcgctgg cgggctggcc ggtttgaaac cggcccctac agcgcgggat
      181 ggcaaggttc gcagtagggg tgggccggtt tcaaaccggc ccctacaggg caggtgggcg
      241 ggatcggcgg ttaaaaatcg tcggcgggcc aggcgatggc gcctggcgcg gcgaagatgg
      301 cggcgatccg cgcgccatcc cagccgggcg cgagcgggag gcccatcagg acgtggtggt
      361 agtcctcgtg gacctgctgg tagatgcccg ccagcgccgc gtcgatcgcc ggctccctgg
      421 ggatatagac tcgccccata ccgatccccc gtgcgcgggc ctcgtccacg accgccgcca
      481 ggagcgccgg gatcgcctcc gccgcgtccg cccgcacgcc gatctccagg acgacaaagg
      541 tctcttccgt gaagtgcgcc agcacgtagc cggcgaccgt cccggcctcg ctcgcgggga
      601 ccgtgaccca gacgcgggca ttgggcgcgc agaacttcac cggcgcgtag cgttcccagt
      661 agggcaggtc gcgcacggtg gtcaacgggc gccgggcgtt gtacgcggcg tagacggcgg
      721 cgagcggcgc ccagccgccc ggcgcgtgcc ggggatcgta gccctgcgtg gtatacgcgc
      781 ccgccggcgt gggttgttcc gcggcgagga ggccctgccg gtagcgtgtg gcgtgggtgc
      841 gccagccgta gcgctcgtaa taggggttga tgtcggtgaa gagcagcgac caggcgcagc
      901 cgtccgcgcg catcgcggca atcgtctgct ccagcaggcg cccggcgtgg ccctggcggc
      961 gcacgtcggg ccgggtggcg acgacggtca gcccgccgac gggctgcggg gcgccgtcga
     1021 gatcgcgcaa ctcgcgccgg tggtaatgca tggccgacag cagtgtgccg tccgcggcca
     1081 ccgccacacg ggtctggtcc cgccgggtgc cggggttgtc cagcaccgca tgccgcaggc
     1141 gctgcgggtc ttcctcgaaa gtctcggccc agagggcgag cacggcggcc tcgtcgccgg
     1201 cgcgcagcgg gcggtagatg gctgctgccg gggtgggttc attactctgc ataggtcaag
     1261 tatacccgca ccgggcgcgg acggcatccg gccccggttg gatggtggtt ccgtctgcag
     1321 ccggatgaaa aggcggacgg cacttttcct gctgtgaggc ggggcagttc acgccgggtg
     1381 aaccccgccc cgcgcccgcc gccgatccgc ggggcgacca tgtgcccggc ctccaaggag
     1441 tgccgcgttc gtgaagatgt ccccgctcgg gatgtaccgc ttttatcacc gcctgtaccg
     1501 gcgcggcatc cccaagctcc cgttctggct ctaccggctg caccggatga tctacgcctg
     1561 tgagatccat cccgccgccg agttaggtcc cggctgccag atgccgcact cggtcggcat
     1621 cgtcatcggg ccggaggtgc gcacggggcg gaactgcttt atcgcgcagg gggtgacgct
     1681 cggctaccgc tatccggggc cgcgcacgcc cgtgcccggc gacggcaacc ccactctggg
     1741 cgacggcgtg gtggtgggcg cgggggccaa ggtcttcggc cccgtcacca tcggcgaccg
     1801 ggccgtgatc ggcgccaaca gcgtcgtcac gcacgcggtg cccgccgggt acctggctgt
     1861 gggcgtgccc gcggagatcc gcccccgtcg ccggtaacgt ctaccggatg ggcgggggcg
     1921 cccggaggct caagcctccg gctaccgcta cgaaggccgc ctgcgcggcc tatcggctca
     1981 gcccgcctgc gcgggcttcg taaagctagc ccgcccgttc acgggccggg cggggccgcc
     2041 gcccggcggg ttgctggcag ggcctggggc ttgctggtat aatggacagg ccggggccgc
     2101 gctcatgcgc gcggtccgcg cgatgtcgcc ggcgtaacga aaggcatgtc tatggactcg
     2161 gttagcgaac atgaggataa attccgcgcc atggccgagc ggcgcatccg cgaggcgctg
     2221 gagtcgggtg agctaaaagt cgagggcggg ccggataaaa aactcgatct ggaagagaac
     2281 ccatttgtgc ccgaggagtg gcggctggcc ttccgcgtgc tcaaaagcgg caactaccgc
     2341 cccgattgga tcgacctggc gaacgacatt gagaacgacc tggccgcctg gcgcaccgcc
     2401 gcggatcgcc actttgcctt cctgcgccgc cggttggatg acctgaccgc cgatgcgggc
     2461 gggttgcgcc gcatgcgcga agaggtcgcc gccctgaagc agcgccacat gcgcgcgacc
     2521 gcgtaccatg cccacatgct cgacgagatc aaccagaaga tccatcgcta taacgccacc
     2581 gtgcccgcca ccagcctgat gcgcccgacg ctcgcgcggg acgacgagat gcgccggttc
     2641 gccgaccggc tgccggccta cctcaactat tagcgcaccg cgcccggtct gccgcctaca
     2701 tgtgccggcc tggatccgcg gagagggtac cccagttatg caagaatctg cttcgccggc
     2761 tgcttccact ctcagcgccg cgcccgcgcg cacccctgcc ctggggccgg acagcgtcac
     2821 ccgccaggtg ctgcccaacg ggctggtggt gctggtctac cccaacccgg ccatcccgac
     2881 cgtgagcgcg cggctcagca tgcgcgcggg cgccctgttc gacccgccgg gcaagagcgg
     2941 cgtcgccggc ctgaccgcgg gcgcgatgcg ccggggcacg cgccgccaca ccttcagcga
     3001 cctcaacgag cgcacggacg agcgcggtat gtcgctcggc ctcgacgcgg gcttccatct
     3061 gctggagatc ggcgggcgca gcttgcgcga agatgcgccg ttcctgatgg agaccctggc
     3121 cgaggtgctg cgcgagccgt cgttcccgtc ggacgaattc gagcgcctgc gcagccagtt
     3181 gatcaccggc ctgatggagc aggaggacga cacccgctcg gtggccgacc gcgatttccg
     3241 ccgcgccgcc tatcccgacg ggcatcccta tggccgcgag gccggcggca ccctggagtc
     3301 cgtgcccacc atcacccgcc aggatctcga agaccactat cgcacctacg tgcgccccga
     3361 cacgggcatc ctggtcatcg tcggcgacat ttcgccggag gccgggctgg acctggcccg
     3421 gcaagccctg ggcgactggc aggtggacgg cgtggcgccc gcgctggtc
//
LOCUS       DASUYC010000329         2600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_936578,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000329 DASUYC010000000
VERSION     DASUYC010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936578"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="VF276_05355"
     CDS             complement(<1..734)
                     /locus_tag="VF276_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6606327.1"
                     /translation="MADTRPVGSNYKWVALSNTTLGVLMAAMNSSIVLISLPAIFRGL
                     NVNPLDPAESGYLLWMMLGYMVITATLLVTLGRLSDMWGRVRLYNLGFVIFTVGSIML
                     FLTPSSGNAGAVEMIISRLVQGVGGGFLMANSAAILTDAFPPNERGMALGLNMVATLA
                     GSLVGLIVGGMMSTVWWRAIFLVSVPIGIFGTVWAYKMLREQGATRAARKIDVLGNLC
                     LGGGLTIFLVAITYGIMPYGGSNTGWG"
     gene            complement(809..1267)
                     /locus_tag="VF276_05360"
     CDS             complement(809..1267)
                     /locus_tag="VF276_05360"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX6606328.1"
                     /translation="MVDNLTDVIEEILDLDSVVQHQLRNSWPESWLQINLPLGSTRAL
                     LAIDGLGAHTPGRVADALGVSRTTVTGLLDRLEGEGLITRTIDPHDRRCFVLHLTEKG
                     QTLVGEINGRRREHIRLALAQMDGESLAALRQGMAALAAAMKAGEPARSK"
     gene            complement(1807..2499)
                     /locus_tag="VF276_05365"
     CDS             complement(1807..2499)
                     /locus_tag="VF276_05365"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX6606329.1"
                     /translation="MGKHLRGVILDVDGTLIDSNGEHAEAWVEAFRQHGYDVPYDKAL
                     HMIGMGSDNLLPKAVGVQKDSPEGQALSKASEDIFKSQYLSHIHAFPQTRALLLHMRD
                     QGLRLAVASSAKEDQLHQLLEIAGAADLIETKTSSGDVKHSKPDPDIVHAALTRLGLA
                     PDEVVMIGDTPYDIEAAGKLGIGTIAFRSGGWDDAGLAGAIAIYDGPADLLAHYAESP
                     LAPDAPPPPPPR"
BASE COUNT          464 a          881 c          807 g          448 t
ORIGIN      
        1 ccccagccgg tattcgaccc gccgtagggc atgatgccgt aggtgatggc gaccaggaag
       61 atggtcaacc cgccgcccag gcagaggttg cccagcacgt cgatcttgcg cgcggcgcgg
      121 gtggcgccct gctcgcgcag catcttatag gcccacacgg tgccgaagat accgatgggc
      181 acgctgacca ggaagatggc ccgccaccac acggtggaca tcatcccgcc gacgatcagc
      241 ccgaccaggc tgcccgccag cgtcgccacc atgttcaggc cgagcgccat gccgcgctcg
      301 ttgggcggga aggcatcggt caggatcgcg gcgctgttcg ccatcaggaa gccgccgccc
      361 acgccttgca ccagccgcga gatgatcatc tcgaccgccc cggcgttgcc gctcgacggc
      421 gtcaggaaca gcatgatcga tcccacggtg aaaatcacaa agccgagatt atacagccgc
      481 acccggcccc acatatcgga caaccggccc agcgtcacca gcaaggtcgc cgtgatcacc
      541 atgtagccga gcatcatcca gagcaaatac ccgctctccg cggggtccag cgggttcaca
      601 ttcaacccgc gaaaaatcgc cggcagcgag atcagcacaa tgctgctgtt catcgccgcc
      661 atcagcacgc caagcgtggt attggagagg gcgacccatt tgtagttcga ccccaccggg
      721 cgtgtatccg ccatcacgat ctcctaccca atcgtcgatt atctaaagca cccttccgtc
      781 cgggccgcgg aacgccggaa ggttgccgtc atttactccg ggccggctcg ccggccttca
      841 tcgccgcggc gagcgccgcc atcccctgcc gcaatgcggc cagggattcg ccgtccattt
      901 gcgccagcgc cagccggatg tgctcgcgcc gccgcccgtt gatttcgccg accagggttt
      961 gccccttctc ggtcagatgc agcacgaagc agcgccggtc gtgcgggtcg atggtgcggg
     1021 tgatcagccc ttcgccttcc agccggtcca gcagcccggt cacggtggtg cggctcacgc
     1081 ccagcgcgtc cgcgacccgc cccggcgtat gcgcgcccag cccgtcaatc gccagcagcg
     1141 cccgcgtgga ccccagcggc aggttgatct gcaaccacga ctccggccag ctattccgca
     1201 actggtgctg gaccacgctg tccaggtcca ggatctcctc gatcacatca gtcagattgt
     1261 ctaccatggt tttataatcc tataaactaa atagtacgga cttctgaatt atatccagat
     1321 ccggcgtatg agtcaagtgg caaacacgcc catgaaagga gtaatgcgca gcctagaggt
     1381 tgtcacgcta agcgcagcga agagtctcgt ccccgcccgg tgtcactctg agcgcagcga
     1441 agagtctcgt cccccgcccg gtgtcactct gagcgcagcg aagagtctcg tcctcgcccg
     1501 gtgtcactct gagcgcagcg aagagtctcg tctcgtatcg ctccaagacg agattcttcg
     1561 ctacgctcag aatgacggat taggaggtgt cacgaaggga tgattgggcg gtccctggtg
     1621 cccataaagg ccgggatgcc ggtgtagggg cgggtttcaa acccgccttg cttccttacc
     1681 catttgctcg ttgacagccc tgctgccggt gtaggggcgg gtttcaaacc caccgggccg
     1741 ccttacccca tgtgccggaa gacagccgcg gcgccggggt aggggcgcgg gccaaacccg
     1801 ccggccctat cgcgggggcg gtgggggcgg cgcgtcgggg gccagcggcg actcggcgta
     1861 atgggccagc agatcggcgg ggccgtcgta gatggcgata gccccggcca ggcccgcgtc
     1921 atcccagccg ccgctgcgga aggcgatggt gccgatgccc agcttgcccg ccgcctcgat
     1981 gtcatacggc gtgtcgccga tcatcaccac ctcgtcgggg gccaggccga gccgggtcag
     2041 cgcggcgtgg acgatatccg ggtcgggctt ggagtgcttc acgtcgccgg acgaggtttt
     2101 ggtctcgatc aggtcggcgg cgccggcgat ttcgagcaac tggtggagtt ggtcttcctt
     2161 ggccgaactg gcgacggcca ggcgcaagcc ctggtcgcgc atatgcaaca acagggcgcg
     2221 ggtctgcggg aaggcgtgga tatgggacag gtattgcgac ttgaagatgt cctccgacgc
     2281 cttgctgagc gcctgcccct cggggctgtc cttctggacg cccaccgctt tgggcagcag
     2341 attatcgctg cccatgccaa tcatatgcag cgccttgtcg tatggcacgt cgtagccatg
     2401 ctgccggaag gcctcgaccc aggcctcggc gtgctcgccg ttgctgtcga tcagggtgcc
     2461 gtccacatcc aggatcaccc cgcgcaaatg cttgcccata agactcctca ctagccgagc
     2521 gccaccatgg cgtacccagc gcgcccaggc cgggcggcgc acccgctact gaggcaagct
     2581 atatgccatg acggacacag
//
LOCUS       DASUYC010000330         7924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_782395,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000330 DASUYC010000000
VERSION     DASUYC010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 7924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7924
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782395"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..1014)
                     /locus_tag="VF276_05370"
     CDS             complement(235..1014)
                     /locus_tag="VF276_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX6606330.1"
                     /translation="MDLPPAAPAASNPDRRVWIGCSGYAYKHWRGPFYPPDLPTTRWL
                     AHYVQFFPTVELNNTYYTMPGPATFESWRDKSPEGFVFAVKASRFITHMKKLKDPQEP
                     LARLFERARLLGPKLGPILYQLPPRWHFDAERLAVFLAALPADLQHAVEIRDPSWLND
                     TFFAALERAHVAFCIASLPQYESPVVATAPFVYIRFHGSRAMYYYDYLADELRVWRDR
                     ILGFVATGHTVYCYFNNDPEGWAVRNALELTQLVNAEAHVA"
     gene            complement(1051..2067)
                     /locus_tag="VF276_05375"
     CDS             complement(1051..2067)
                     /locus_tag="VF276_05375"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HEX6606331.1"
                     /translation="MARSTVKPGTEDTETAPPEVAEHATAAGPEIALPSEPGRRRPWP
                     LLLLKTMRPKQWTKNGFVLAALIFAFRFRDPQALALTAAAFILFCLLSSAVYIMNDLA
                     DREADRKHPRKRYRPLASGELNPSIATAACVVILVGTLGVAFAINWMFGAICVLYFAT
                     QVGYSFWLKHVVIVDVLLLASGFVLRAMAGALVIDVPISPWLYVCVTLLGLFIGCSKR
                     RHELILLSEGAGSHRRILQEYSPVLLDQMITVVTSSTVMAYAMYTFTAKNLPENHLMM
                     VTIPFVLYGIFRYQYLIYQKNEGGAPDELLLRDRPLLVCVILWGLTAVAVLTLARIYN
                     LPLQ"
     gene            2823..3113
                     /gene="groES"
                     /locus_tag="VF276_05380"
     CDS             2823..3113
                     /gene="groES"
                     /locus_tag="VF276_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX6606332.1"
                     /translation="MGNLKPLGDRVVVKALEREEVTKSGIYLPDTAKEKPQEGRVEAV
                     GPGRILDNGERSKMELKEGDRVLFAKYAGTEFKLDGEDLLILRESDILAVIS"
     gene            3155..4795
                     /gene="groL"
                     /locus_tag="VF276_05385"
     CDS             3155..4795
                     /gene="groL"
                     /locus_tag="VF276_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258594.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX6606333.1"
                     /translation="MAKQIEFNEEARRSLKRGVDTIANAVKTTLGPKGRNVALDRKYG
                     APTVTHDGVTVAKEIELKDPFENMGAQLLKEAATKTNDIAGDGTTTATVLAQAIVNEG
                     LRNVAAGANPMLLKRGIDQGVNALVERIRENAKPVNSQEEIASVATISAADAEIGRLI
                     AEVMEKVGKDGVITVEESRGLKFETEYTEGMQLDRGYISSYFVTNADRMEAVLDDPYI
                     LITDKKISALSDLLPVLEKFLQISKNLLIIAEDIDGEALATLVVNRLRGVLNVVGVKA
                     PGFGDRRKEMLQDIAILTGGQVISEDIGRKLDSTTVKDLGRARRVVVTKDETTIIEGK
                     GDEAAIRARVDQIKTLIEQTTSDYDREKLQERLAKLAGGVAVIKVGAATETELKEKKH
                     RVEDALSATRAAVEEGIVPGGGVALLNALPALDSIKADGDIATGVSILRRALEEPMRQ
                     IALNAGQDGAVVVETVRRKATDENNASVGYNVLSGQYGDMVAQGIIDPAKVTRSAVEN
                     AASIAGMILTTEALITDIPEEKPAMPAGGGMPGGGMDY"
     gene            complement(5021..6235)
                     /locus_tag="VF276_05390"
     CDS             complement(5021..6235)
                     /locus_tag="VF276_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX6606334.1"
                     /translation="MSMTSAAGSPPALVTDPGRWSAHDDLLRYRPDFPIFEKVNYLNS
                     CSLGALSVRAMAGVNEYLTLWGSMGASAWYEIWLGRLAALRTNLGALINAGADEIAIG
                     PSISGAVSVIASCLDYSKRNKVVMADLDFPTLGHQWLAKRKQGVEVEFVPSRNRVSVD
                     LEAFERAVDERTALVATSHVFFTSGFVQDIKAIAEIAHRKGALVLIDAYQATGQIPTD
                     VKDADVDFLLSGGLKWLLGGTGIVFTYARRELIPQLEPTIPGWFAVNNQFGFDIHALE
                     YRPDAGRLEQGTPSISAVYALLGGLSYVQEIGPPALRERTQHLSEDLIARALAAGFVP
                     RVAEDPAQRSGIVLLNMDNPRPVVAALAQAGTIVDMRPGAVRVSPYFYNTIEENQVVI
                     DAMVQIRDQAGS"
     gene            complement(6571..>7924)
                     /locus_tag="VF276_05395"
     CDS             complement(6571..>7924)
                     /locus_tag="VF276_05395"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEX6606335.1"
                     /translation="NYAAAANKTFEVGSANGYSPVVVNATAGTFPADVTLRAVEGTMP
                     GLVDASRSLQRYWGITAGDLTADLTFNYLDPTDIPATADENSFVIFMNDGTLSQPGGT
                     VTPASNQATITGAPGLTALWSLAEPIAAATFTPTPTGVPTNTPTATHTATATNTPVPP
                     SHTPTPTVTVGAPTHTPTPTGTAGVPTSTPTGTSVAATPTGTALVPTNTPTATPAAPT
                     STPGLTQTAVPSPTGAATQTPASTPTVPPCTISFSDVDPANYFYEPVRYLYCNGVISG
                     YADNTFRPYNNTTRAQMVKIVVLGFGIPDQVPTGEAHTFADVLPAHPFYRYIETAAAR
                     GIVSGYNCGPGGPGPCDDQNRPYFAPNNPVTRGQLSKIDAVAAGWPLQNPPSATFNDV
                     PRDSPFYTYIETAVCRGVISGYDDHTFRPANNATRGQISKIVYLSITAGPGTCAAPNP
                     "
BASE COUNT         1403 a         2410 c         2800 g         1311 t
ORIGIN      
        1 ccagcggggg ggcggcgggg gccgcgtcgt tgtgattcgc catagtttgt ataacgttgc
       61 tccctggctg ctaaagttcc gtttaccaac tattatacca aacttgacat aagcaaagca
      121 aactcgttag tttgtcactc tgagcgcagc gaagcgtctc gtctgttgag ctacgagacg
      181 gagattcttc gctgcgctca gaatgaccga tcccggtggc cggactacgc atgttcaagc
      241 gacgtgcgct tcggcgttga ccaactgcgt cagttcgagc gcgttgcgga cagcccagcc
      301 ttccgggtcg ttgttgaaat agcaatagac cgtgtgcccg gtggcgacaa agcccaggat
      361 gcggtcgcgc cagacgcgga gctcgtcggc caggtagtca tagtaataca tcgcgcggct
      421 gccgtgaaag cggatgtaga cgaacggcgc ggtggcgacc accgggctct cgtattgcgg
      481 caggctggcg atgcagaaag cgacgtgcgc ccgctccagc gcggcaaaaa acgtgtcgtt
      541 cagccagctt gggtcgcgga tctcgacggc gtgttgcaga tcagcaggca gcgcggccag
      601 gaacacggcc aggcgctcgg cgtcgaaatg ccagcgcggc ggcagttggt acaggatcgg
      661 ccccagcttc gggccgagca accgcgcccg ctcgaacagg cgcgccagtg gctcctgcgg
      721 gtccttcagc ttcttcatgt gcgtgatgaa gcggctggcc ttgaccgcga agacgaagcc
      781 ctccggcgac ttgtcgcgcc aggactcgaa tgtggccggg ccgggcatgg tgtaataggt
      841 gttgtttagc tcgacggtgg ggaagaactg gacatagtgg gccagccagc gcgtggtggg
      901 caggtccggc gggtaaaacg gcccgcgcca gtgtttgtag gcgtagccgg aacagccgat
      961 ccagacgcgc cggtcggggt tgctcgccgc cggggccgcg ggtggtaagt ccatggcggc
     1021 ccacgagcaa ttgcggtgcc agggccctag ctactgcaag ggcaggttgt agatgcgcgc
     1081 cagggtcagc acggccacgg cggtgaggcc ccagaggatg acgcagacca gcagcggccg
     1141 gtcgcgcagc agcagttcgt cgggcgcgcc tccctcgttc ttctggtaga tcaggtactg
     1201 gtagcggaag atgccgtaga ggacgaaggg gatcgtcacc atcatcaggt ggttctccgg
     1261 caggttcttc gccgtgaagg tatacatggc ataggccatg accgtgctcg acgtgacgac
     1321 ggtgatcatc tggtcgagca gcaccggcga gtactcttgc aggatgcggc ggtggctgcc
     1381 cgccccctcg ctcagcagga tcaactcgtg gcggcgcttg ctgcacccga tgaacaggcc
     1441 gagcagcgtc acgcagacat agagccaggg cgagatcggc acgtcgatga ccagggcgcc
     1501 ggccatggcc cgcagcacga agccggacgc cagcagcagc acgtctacga tgacgacatg
     1561 cttgagccag aacgagtagc cgacctgggt ggcgaaatag agcacacaga tcgcgccgaa
     1621 catccagttg atggcgaagg ccacgcccag cgtgcccacc aggatgacca cgcaggccgc
     1681 ggtggcgatg ctgggattca actcaccgga cgccaggggc cggtagcgct tgcgcgggtg
     1741 cttgcggtcg gcctcgcggt cggccaggtc gttcatgatg tagaccgcgc tcgacagcag
     1801 gcagaagagt atgaacgcgg cagccgtcag cgcgagggcc tgggggtcgc ggaagcggaa
     1861 ggcgaagatg agggcggcca gcacaaagcc gttcttggtc cactgcttgg gccgcatcgt
     1921 cttgagcagc agcagcggcc aggggcgccg gcgtccgggc tcgctgggca gcgcgatctc
     1981 cggcccagca gcggtggcgt gctccgccac ctccggcggc gccgtctcgg tatcctcggt
     2041 ccctggtttc actgttgaac gcgccatcgc cggcacactg cctcctgcta aaatcgacta
     2101 aacatcttat catacccgaa aggtgccccg cctgcaaccg ccggatgccc gtcctgggag
     2161 gcgagatcgc ccggtccgtc aacgggcacg cgatagaggg atcgccgggc ccgtcaacgg
     2221 gcgggctagc cctacgaagc ccgcctgcgc cagctgggtt gtgttcaagc aacctcttgt
     2281 gtgcagtgat gcgtaggacc gcgggtcgtg ttcggtttgg gtgtaaccgc gtgaaggatg
     2341 gcgcggcggg cttgggttgg gtgtagctgc gcgatttatc gcgccgtact cgttgctcgt
     2401 tagcgtgctc tgccaggaat agatggcgcg tgctgcgccc ggctggttcc tggcacgggc
     2461 tgggcgacca atacgctggc gataaacgag tacggcgcga taaatcgcgc ggctacacaa
     2521 tggctaatcg gtagttgtgc caaggcaggc cgcgcaggcg gccttcgtaa gggtagcggg
     2581 cggcttgagc cgccccggcg gcgcctcccg gccaggattc tattagcact ctaacaaact
     2641 gagtgctaaa attggcccgc aaacccttgc aatcggcggc gttttgctgt atattggggt
     2701 tagcactcag acgacgagag tgctaacccc gtcaaaagta gcgtgcgccg ggaaactgtg
     2761 cgccgcgtgc ggcttattga cacatttcca ccgaggccgg tggatgcaag gaggttcgtt
     2821 ggatgggaaa cctgaaaccg ttgggtgatc gcgtggtggt caaggcgctg gagcgcgaag
     2881 aagtgaccaa gagcggcatt tatctgccgg atacggccaa ggagaagccc caggagggcc
     2941 gcgtcgaggc cgtcggtccg ggccgcatcc tcgataacgg cgagcgctcg aagatggaac
     3001 tcaaggaagg cgatcgcgtg ctcttcgcga agtatgccgg gaccgagttc aagctggacg
     3061 gcgaggatct gctgattctg cgcgagagcg atattctggc tgttatcagc taaccttgta
     3121 ttagactcag tttcatcagg aggatttagg ttttatggct aagcagatcg aattcaatga
     3181 ggaagcccgg cgctcgctga agcgcggtgt ggacacgatc gccaacgcgg tcaagaccac
     3241 cctcggcccc aaggggcgca acgtggccct cgaccgcaag tatggcgcgc cgaccgtcac
     3301 ccacgacggc gtgaccgtcg ccaaggagat cgagctgaag gaccccttcg agaacatggg
     3361 cgcccagctc ctcaaggaag ccgccaccaa gaccaacgac atcgccggtg acggcactac
     3421 caccgcgacc gtgctcgcgc aggcgatcgt caacgagggt ctgcgtaacg tggccgccgg
     3481 cgccaacccc atgctgctca agcgcggcat cgaccagggt gtgaacgccc tcgtcgagcg
     3541 catccgcgag aatgccaagc cggtcaactc gcaggaagag atcgccagcg tcgccaccat
     3601 ctccgccgcc gatgccgaaa tcggccgcct gatcgccgag gtcatggaga aggtcggcaa
     3661 agatggcgtc atcaccgtcg aggagagccg cggcctcaag ttcgagaccg agtataccga
     3721 gggcatgcag ctcgatcgcg gctacatcag ctcctatttc gtcaccaacg cggaccgcat
     3781 ggaggccgtg ctggacgacc cctacatcct gatcaccgac aagaagatct ccgccctcag
     3841 cgacctgctg cccgtgctgg agaagttcct ccagatcagc aagaacctgc tgatcatcgc
     3901 cgaggacatc gacggcgagg ccctcgccac cctggtggtg aaccgcctgc gcggcgtgct
     3961 caacgtcgtc ggcgtgaagg cccccggctt cggcgatcgc cgcaaggaga tgctgcagga
     4021 catcgccatc ctgaccggcg gccaggtcat cagcgaggac atcggccgca agctcgacag
     4081 caccaccgtc aaggacctgg gccgcgcccg ccgcgtcgtc gtgaccaagg acgagacgac
     4141 gatcatcgag ggcaagggcg acgaggccgc gatccgcgcc cgcgtcgacc agatcaagac
     4201 cctgatcgag cagaccacca gcgactatga tcgcgagaag ctgcaggagc ggctggcgaa
     4261 gctggccggc ggcgtggccg tcatcaaagt tggcgccgcg accgagaccg agctgaagga
     4321 aaagaagcac cgcgtcgagg acgccctgag cgctacccgc gccgcggtcg aagagggcat
     4381 cgtgcccggc ggtggcgtcg cgttgctgaa cgcgctgccc gccctggatt cgatcaaggc
     4441 cgacggcgat atcgccaccg gcgtctcgat cctgcgccgc gcgctcgaag agccgatgcg
     4501 ccagatcgcg ctcaacgcgg gccaggacgg cgcggtcgtg gtcgagacgg tgcgccgcaa
     4561 ggcgaccgac gagaacaacg cctccgtcgg ctacaacgtg ctgagcggcc agtatggcga
     4621 catggtggcc cagggcatca tcgacccggc caaggtgacc cgctcggcgg tggagaatgc
     4681 ggcttcgatt gccggcatga tcctgaccac cgaggccctg atcaccgaca ttccggaaga
     4741 gaagcccgcg atgccggccg gcggcggcat gcccggcggc ggcatggatt actaagtcct
     4801 gagtgctgct cgcgcagcgg tgcgtagcac attgctgagt gctgagtagt ttacgagggt
     4861 tcaatggctt ggcttcgggc tgagtaagtt agggaagtaa gagagagtcc ctgatccaaa
     4921 cggatcaggg actcttttta tatggcggaa aacaagactt atacagataa tcttcgcgtc
     4981 cttcgcgtct tcgcggtgaa gttccgtccg gatgcgcggg ctagctgccg gcctggtcgc
     5041 ggatctgcac catggcgtcg atgacgacct ggttctcctc gatggtgttg tagaagtagg
     5101 gcgagacgcg gacggcgccg gggcgcatgt ccacgatggt gcccgcctgg gccagcgcgg
     5161 cgaccaccgg gcgcgggttg tccatgttga gcagcacgat gcccgaccgt tgagccgggt
     5221 cctcggccac gcggggcacg aacccggcgg ccagcgcgcg cgcgatcagg tcttcgctga
     5281 ggtgctgcgt gcgttcgcgc agcgcgggcg gcccgatctc ctgcacatac gagaggccgc
     5341 cgagcagtgc gtagacggcg gagatggacg gcgtgccctg ctccagccgc ccggcgtcgg
     5401 gccggtattc gagcgcgtgg atgtcgaagc cgaactggtt gttgacggcg aaccagccgg
     5461 ggatggtcgg ctccagttgc gggatcaact cgcgccgggc gtaggtgaag acgatgcccg
     5521 tgccgcccag cagccacttg agcccgcccg acagcaggaa atcgacatcg gcatccttca
     5581 cgtcggtcgg gatctggccg gtggcctggt aggcgtcgat cagcaccagg gcgcccttgc
     5641 ggtgcgcgat ctcggcgatg gccttgatgt cctgcacaaa gccgctggtg aaaaagacgt
     5701 gtgacgtggc gaccagcgct gtgcgctcat ccacggcccg ctcaaacgcc tccaggtcca
     5761 cgctcacgcg gttgcgcgag ggcacaaact ccacctcgac accctgcttg cgcttggcga
     5821 gccactggtg gcccagcgtg gggaagtcga ggtcggccat gaccaccttg ttgcgcttgc
     5881 tataatccag gcaggacgca atgaccgaca cggcgcccga gattgacggg ccgatagcga
     5941 tctcgtccgc cccggcgttg atcagcgcac ccaggttggt ccgcagcgcg gccagccggc
     6001 ccagccagat ctcataccag gccgacgcgc ccatgctgcc ccacagcgtc aggtactcgt
     6061 tgactccggc catggcgcgc accgacagcg cgccgagcga acaggagttg agatagttca
     6121 ccttctcgaa gatcgggaaa tccgggcgat agcgcaacag gtcgtcgtgc gcggaccagc
     6181 ggccgggatc ggtgacaagc gcgggcggcg accccgccgc cgaagtcatg ctcatagtgc
     6241 ggtgcctttc atactggccg ggcggcagcg cggggcatgt tggtcccgcc ggggggccgc
     6301 ccgaagggtg agtggccgtg gcccagagcg tagcacaaag tgcgccctgt ttcaacatcc
     6361 tggtgggggc caaccttacc cccggcccct ctccgccgcg gagaggggag tcgtcttttc
     6421 ccccttcccg cgccgggaag gggggcaggg gggttaggtt ggtcgggcga gaaacaaaaa
     6481 accatccagc gctccccggc aagggcgatc cctggatggc ggtggggggc ggcgcctcag
     6541 tgacgaggcg ccgccccgga gcttacggat ttacgggttg ggggcggcgc aggtgcccgg
     6601 tcccgcggtg atcgaaagat agacgatctt gctgatctgc ccgcgggtcg cgttgttggc
     6661 cgggcggaac gtgtggtcgt catagccgct gatcaccccg cggcagacgg ctgtttcgat
     6721 gtaggtgtag aaggggctgt cgcgcggcac gtcgttgaag gtggccgacg gcggattttg
     6781 cagcggccag cccgccgcca ccgcgtcgat cttcgagagc tgcccgcggg tgacggggtt
     6841 gttgggcgcg aagtaggggc ggttctggtc gtcgcaggga ccggggccgc ccggcccgca
     6901 gttgtagccg ctgacgatgc cgcgggccgc cgcggtctcg atgtagcggt agaacgggtg
     6961 cgcgggcagc acgtcggcga aggtatgggc ctcgccggtg ggcacctggt cggggatgcc
     7021 gaaacccagc accacgatct tcaccatctg cgcgcgggtg gtgttgttgt acggccggaa
     7081 ggtgttgtcg gcgtagccgc tgatcacccc attgcagtac aggtagcgca ccggctcgta
     7141 gaagtagttc gccgggtcca catcgctgaa cgagatcgtg cagggcggca ccgttggcgt
     7201 gctcgcgggc gtctgggttg ccgcgccggt cgggctggga accgccgtct gggtcaggcc
     7261 cggcgtgctg gtgggcgcgg cgggggtcgc ggtgggcgta ttcgtgggca ccagggcggt
     7321 gccggtgggc gtggccgcga cagaggtgcc ggtcggcgtg ctggtaggca cgccagcagt
     7381 gcccgtcggc gtgggcgtgt gcgtcggcgc gcccaccgtc acggtcggcg tgggggtatg
     7441 cgacgggggc accggcgtat tggtcgccgt cgcggtgtga gtggccgtgg gcgtgttggt
     7501 cggcaccccg gtcggcgtgg gcgtgaaggt cgccgcggcg atcggctcag ccagcgacca
     7561 gagggcggtg aggccgggcg cgccggtgat cgtggcctgg ttgctcgccg gggtgaccgt
     7621 gccgcccggt tggctgagcg taccgtcatt catgaagatg acgaagctgt tttcatccgc
     7681 cgtggccggg atgtcggtcg ggtcgagata gttgaacgtc aggtcggcgg tgaggtcgcc
     7741 ggcggtgatt ccccagtagc gctggagcga ccggctcgca tcgaccaggc cgggcatggt
     7801 gccctcgacc gcccggaggg ttacgtcagc cgggaaggtg ccggcggtgg cgttgacgac
     7861 cacgggcgag tagccgttgg ccgagccgac ctcgaaggtc ttattggcgg cagcggcgta
     7921 gttc
//
LOCUS       DASUYC010000331         3237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1661558, whole genome shotgun sequence.
ACCESSION   DASUYC010000331 DASUYC010000000
VERSION     DASUYC010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1661558"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(439..1473)
                     /locus_tag="VF276_05400"
     CDS             complement(439..1473)
                     /locus_tag="VF276_05400"
                     /inference="COORDINATES: protein motif:HMM:NF037672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector HopA1 family protein"
                     /protein_id="HEX6606336.1"
                     /translation="MLSDSFPTPATPFLAELTALIGAVQIQTPREIFFAARPIDLGPP
                     DWPVAFPPPLPPLLTGLYYRLYREGYCARYQGAPAPDSAPDGPDADFIRSLEAANPGK
                     TRMDAGWHVVGPGPDGRFWLQKGGVARTAWPGAFLTHAGPNQAPQAGQPASIHWPHES
                     RQLQPGTYFVIGAAVPDDVADAPIMRFYWHVQAGGAPRLLAALAAALNRFQIPFRFKC
                     PAHPANYSRRDAAVLYISRRDYALVARLLAPIYYNLQACMQPDTPLFTRRLAPGLAFA
                     EDPGTEESFGQHRCRLLAGGLWRAHELQAMSSAERLEIIRKHFAETGIDLDRPHLNAT
                     TREDYPWPPA"
     gene            complement(1583..2641)
                     /locus_tag="VF276_05405"
     CDS             complement(1583..2641)
                     /locus_tag="VF276_05405"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX6606337.1"
                     /translation="MLLTAANLFYYLHQGGLITPGALVAGDFCVVPGGSRHRTFAVPR
                     EQGPGYFVKQVKQWEPDTGQTLQTEAGWLRAATDDPLLAPLGSYVPRFYAYDPGSRVL
                     VVEWLAGTPLSLHIARQGKLNLELAAELGTALGTLQTRLAAGWPGGHPPAHFVPIRLW
                     VLNEAGLPTGSSTQSPGQAEVLAALRRYPAFATRLADLRTTWEPTMLIHADLKWDNWL
                     LCRPALKIVDWEFAGIGDPAWDAGTLAQTFLTWWVGNAANPEAIEPDPPAQTQAAIRA
                     FWTAYIAARGLNSVAARHLLDRTIICAAARLIQTAYESMYHSTQLSTIAVRLLQLSLN
                     ILEDPQAAIHELWGSPAA"
     gene            complement(2674..>3237)
                     /locus_tag="VF276_05410"
     CDS             complement(2674..>3237)
                     /locus_tag="VF276_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606338.1"
                     /translation="GLEYFWLMWDANNITYHADGGVTLTIPGIAGGAATTNYYPAEGV
                     IVLRGYVGLNDADTTKFLLYFDVDLTHYLEIPNDSFRYGVQLDIDKSPLKLCYIWIDR
                     GAQVTEHRTAVQRSYTAGLLEGVVVQDYLGGAVGAGADPMVDRPNPGGAGGWTSAEPG
                     CPPSSGCPSSPGGHCGGAPVVSGLPTC"
BASE COUNT          603 a         1014 c         1043 g          577 t
ORIGIN      
        1 gatggccccc aacgcggtgt gccggtaaac ggactccccc gtcagcttgt atagctggcc
       61 caggaagagg gcgatcccgc tggtgcctcc atacaagtca ggcccaccgg cccggtagat
      121 taccgccggt cgcgcgggat ccccaatcac tgcaggtcca agccaattgc aggcagctcc
      181 tgcccatatc gcatcccggc acaggcgcgc gccgatccga tctgcggctt cgagaaaaag
      241 gttgttacca ggcatggtca tagtatatgt ggcgccatcg acctaatagc cggtcacagt
      301 gctgttgagt tgtaggggcc ggtttcaaac cggcccgccg cccagcggca gagaccgatc
      361 tcaaaccggc ccgctgcccc gccgtagggg ctggtttgaa accggcccta gcgcccccga
      421 aagcaccatg tgcgattcct atgccggcgg ccatgggtag tcctcgcgcg ttgtggcgtt
      481 gagatgcggt cgatccaggt cgatgccggt ttcggcgaaa tgcttgcgga taatttccag
      541 ccgctccgcg ctcgacattg cttgtaactc gtgggcccgc cagaggcccc cagccagcag
      601 gcggcaacga tgctggccga agctctcctc cgtccccgga tcctcggcaa aggccagacc
      661 cggcgcaagc cggcgggtaa agagaggggt atcaggctgc atgcaggctt gcaagttgta
      721 gtagatcggc gccagcaggc gggcgactag cgcgtagtcg cgccgactga tgtagagcac
      781 ggcggcgtcg cggcggctat aattggccgg gtgcgccggg catttgaagc gaaagggtat
      841 ctggaagcgg ttaagcgcgg ccgcgagggc cgccagtagt cggggcgcgc cgccggcctg
      901 aacatgccag taaaagcgca tgatcggcgc atcggccacg tcatcgggca cggctgcgcc
      961 gatcacaaaa taggttccag gctgcaactg ccgggactcg tgcggccagt ggatactggc
     1021 cggttgcccg gcctgcggcg cctgattcgg accggcgtgg gtcaagaatg cacccggcca
     1081 cgctgttcga gccacgccgc ctttttgtaa ccagaatcgc ccatccggcc ctggccccac
     1141 cacgtgccag cccgcatcca tgcgtgtctt acccggattc gccgcctcca acgagcggat
     1201 gaagtccgcg tcgggtccgt caggcgcgga atctggcgcg ggggcgccct ggtaacgcgc
     1261 gcaatagcct tcgcggtaca agcggtaata tagccccgtt aagagcggtg gaagcggagg
     1321 cggaaacgcg accggccagt ccggtggccc gagatcgatc gggcgggcgg caaagaagat
     1381 ttctctcggg gtctggatct ggacggcacc gatcaacgcg gttagctcgg cgagaaacgg
     1441 tgttgcgggt gttgggaatg agtcgctcag catggtcgcg tatcctcgct tcttatgccc
     1501 cgcccgccgt cccgcgcgcc gattcgcctg ggtggcaggt tggtttccag ccggcttcat
     1561 cccggcaaag gatcggtgcc ggctacgcgg cgggcgatcc ccacaactca tgaatggccg
     1621 cctgcggatc ttctaaaatg ttgaggctta gttgcaacag acgcaccgct atggttgaaa
     1681 gctgagtgct gtgatacatc gattcgtagg cggtctggat aagccgcgcc gccgcgcaga
     1741 taatcgtccg atcgagtaag tgtcgcgcgg cgaccgagtt caggccgcgg gcggcgatgt
     1801 aggcggtcca gaaggcgcgg atggcggcct gtgtctgcgc cggcgggtcc ggttcaatgg
     1861 cttctggatt ggcggcgttg cctacccacc aggttaggaa ggtttgcgcc agcgtgccgg
     1921 cgtcccatgc tggatcgcca atcccggcga attcccaatc cacgatcttc aacgccggtc
     1981 tgcatagcag ccagttgtcc catttgaggt cggcgtgaat gagcatagtt ggttcccagg
     2041 tggtacggag gtccgccaac cgggtcgcga acgccgggta tcgccgtaag gcggccagca
     2101 cctcggcctg gccgggactc tgcgtggagc taccggtggg caacccggcc tcgttaagta
     2161 cccagagccg gatcggcacg aaatgagcgg gagggtggcc gccgggccac ccggcggcca
     2221 gtcgagtctg gagggtgccc agcgccgtcc ccaattcggc cgccagttcc aggttcagct
     2281 taccctggcg ggcaatgtgc aacgagagtg gtgtgccggc cagccattcg accaccagca
     2341 cccggctgcc cggatcatag gcgtaaaaac gcggtacata tgatccaagc ggggccagta
     2401 acggatcgtc cgtcgctgcg cgcaaccagc cggcctcggt ttgtagcgtt tgaccggtgt
     2461 ccggctccca ctgcttcacc tgcttcacaa agtagcccgg cccttgctca cgcgggaccg
     2521 cgaacgtgcg gtggcggctg ccgccgggca cgacacagaa atctccggcc accagggcgc
     2581 ctggcgtgat caagccgccc tggtgcaggt aataaaagag attggcagcc gtcaggagca
     2641 aggatgtaca gtacccttat ccaccaaggg ctattaacag gtgggcaacc cggataccac
     2701 tggcgcgcct ccacagtgac cgccaggact cgaaggacag ccgctcgaag gggggcagcc
     2761 aggctccgcg ctcgtccacc cgccagcgcc gcctgggttg ggccggtcga ccatcgggtc
     2821 tgctccggct ccgactgccc ctccgagata atcctgcacg acgacacctt cgagcaaccc
     2881 ggcagtgtac gaacgctgca ccgcggtgcg atgttcagtt acctgcgcgc cccgatctat
     2941 ccaaatgtag cacagtttca gcggagactt gtctatgtcc aattgaacgc catagcgaaa
     3001 gctgtcgttc gggatctcca ggtagtgggt gaggtccacg tcgaagtaga gaaggaactt
     3061 ggtcgtatct gcgtcattca agcctacata gccgcgcagc acgatcacac cttcagcggg
     3121 gtagtaattc gtggttgccg ctcctcccgc tatgcccggg atggtcagag ttacgcctcc
     3181 atcggcgtga taagtgatgt tattcgcatc ccacatgagc cagaagtact caagacc
//
LOCUS       DASUYC010000332         3745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1806498, whole genome shotgun sequence.
ACCESSION   DASUYC010000332 DASUYC010000000
VERSION     DASUYC010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1806498"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..438)
                     /gene="rpsH"
                     /locus_tag="VF276_05415"
     CDS             complement(40..438)
                     /gene="rpsH"
                     /locus_tag="VF276_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX6606339.1"
                     /translation="MNTTDPIADMLTRIRNGGMARHQSVLIPTSKMKLAIARILKEEG
                     YIKDFDFPGEDGNRNFRVWLKYGPDKKPVVTGLKRVSKPGLRVYANRQEIPVVLGGLG
                     VCVLSTSQGILSGHEAWRKRVGGEVLCYVW"
     gene            complement(457..642)
                     /locus_tag="VF276_05420"
     CDS             complement(457..642)
                     /locus_tag="VF276_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEX6606340.1"
                     /translation="MAKTSLIVKASRPAKYKVRAYNRCKVCGRPRGYMRKFGLCRICF
                     REMALQGKLPGVVKSSW"
     gene            complement(664..1218)
                     /gene="rplE"
                     /locus_tag="VF276_05425"
     CDS             complement(664..1218)
                     /gene="rplE"
                     /locus_tag="VF276_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEX6606341.1"
                     /translation="MAEYVARQREKYYKEVVPALMKEFGYTNPMQVPRVTKIVLNIGM
                     GEAIANAKALDNAVNDVTAIVGQKPMITRAKKSIAAFKLRAGMPIGVMVTLRSERMYD
                     LMDRLFNIALPRVRDFSGVNTKSFDGHGNYTLGLREQLIFPELEYDRIDKVRGMELVF
                     VTTAKTDQEARRLLELMGMPFRRS"
     gene            complement(1376..1714)
                     /gene="rplX"
                     /locus_tag="VF276_05430"
     CDS             complement(1376..1714)
                     /gene="rplX"
                     /locus_tag="VF276_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEX6606342.1"
                     /translation="MNVHKDDTVLVIAGKDKGKRGRVREVHPSDNKVIVEGINVMRKH
                     TRGRAGARQAGIVEVEAPMDASKVMVVCAKCNKPVRVAHTFLADGSKARSCRNCNELL
                     DRDPDKRWSR"
     gene            complement(1831..2199)
                     /gene="rplN"
                     /locus_tag="VF276_05435"
     CDS             complement(1831..2199)
                     /gene="rplN"
                     /locus_tag="VF276_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEX6606343.1"
                     /translation="MIQPQTRLRVADNTGAKEIMCIRVMGGSNRKYAGVGDIIIAAVK
                     QAAPGGAVKKGEVVRAVVVRTAKEYGRPDGSHIRFDDNAAVILNPQNNPRGTRIFGPV
                     ARELRERQFMKIVSLAPEVL"
     gene            complement(2235..2495)
                     /gene="rpsQ"
                     /locus_tag="VF276_05440"
     CDS             complement(2235..2495)
                     /gene="rpsQ"
                     /locus_tag="VF276_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEX6606344.1"
                     /translation="MPEHGKSRMRTGRVVSNKMDKTVIVAVDRLVRHRLYKKIMRRTN
                     KFVAHDELKCNEGDIVRILETRPISKTKRWRVVEILSRAPQI"
     gene            complement(2678..2920)
                     /gene="rpmC"
                     /locus_tag="VF276_05445"
     CDS             complement(2678..2920)
                     /gene="rpmC"
                     /locus_tag="VF276_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEX6606345.1"
                     /translation="MQASEIRDMNEAEIQRHLDEYRHELFNLRFQIATRKLKNHQRVK
                     TVRRDMARLLTVIREREIEALYAAEIAELQALNASL"
     gene            complement(2907..3374)
                     /gene="rplP"
                     /locus_tag="VF276_05450"
     CDS             complement(2907..3374)
                     /gene="rplP"
                     /locus_tag="VF276_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEX6606346.1"
                     /translation="MLMPKRMKHRKVHRGTTGAGKAQRGNTVAFGEYGLQALEAAWID
                     SRQIESARRAITHYVKRGGKVWIRIFPDKSMTQRPAETRMGSGKGAPDHWVAVIRPGR
                     ILFELGGVREDVAAEAMRLAGHKLPIKTRFVARIAEGAVTHEETDEETSDAGE"
     gene            complement(3378..>3745)
                     /locus_tag="VF276_05455"
     CDS             complement(3378..>3745)
                     /locus_tag="VF276_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606347.1"
                     /translation="PAPAAETTPSAPAAETAIQVSHEALPAPAAPTAEQPAPPSMETS
                     MPAAAPAEEPATPYVAVETQGDTATPATDLGIASEAPATAEAAGTGAGSLEASTENSA
                     ASDAGTPSADEAAQPGEES"
BASE COUNT          570 a         1247 c         1177 g          751 t
ORIGIN      
        1 acgccggcac gcgcccggct gcactgcaca gcagtacgtc taccagacgt agcagagtac
       61 ttcgccgccc acccgcttgc gccaggcttc gtggcccgac aggatgccct gggaggtcga
      121 cagcacgcac acgcccaggc cgccgagcac caccgggatc tcctggcggt tggcatagac
      181 ccgcaggccc ggcttgctga cccgcttaag gccggtgaca accggcttct tgtcggggcc
      241 atacttcaac cagacgcgga agttgcggtt gccgtcctcg cccggaaaat cgaaatcttt
      301 gatatagccc tcctccttca agatgcgggc aatcgccagc ttcatcttgg aggtcggaat
      361 caacacactc tggtgccggg ccatgccgcc gttgcggatt cgggtcagca tatcggcgat
      421 cggatcagtc gtattcatga actacttgtt ctcctcttac caactcgact tcacgacgcc
      481 gggcagcttg ccctgcaacg ccatctcgcg gaagcagatg cggcacaggc caaacttgcg
      541 catgtagccg cggggacgcc cgcagacctt gcagcgattg tacgcccgca ccttgtactt
      601 ggccgggcgg gacgccttca cgataagcga ggttttcgcc attccctgtg tccttatcct
      661 ttcctatgag cgccggaagg gcatgcccat gagttccaac agccgccgtg cttcctgatc
      721 ggttttcgcg gtggtgacaa agaccagttc catgccgcgg accttatcga tccggtcgta
      781 ctccagctcg gggaaaatca gttgctcgcg caggcccagc gtgtagttgc cgtggccgtc
      841 gaacgacttg gtgttcaccc cgctgaagtc gcgcacacgc ggcagggcga tattgaacag
      901 gcggtccatc aggtcataca tgcgctcgct gcgcagcgtg accatgacgc caatcggcat
      961 gccggcgcgc agcttaaagg cggcaatcga cttcttggcc cgcgtgatca tcggcttctg
     1021 gccaacgatg gcggtcacgt cgttcaccgc attgtcgagc gccttggcat tggcgatggc
     1081 ttcgcccatg ccgatgttga gcacgatctt ggtgacgcgc ggcacctgca tcgggttggt
     1141 ataaccaaac tctttcatca gcgccggcac gacttcctta tagtacttct cgcgctgacg
     1201 ggcaacgtat tctgccactc tacatcctcc caccggcacc gggcctgccg cccgccggtt
     1261 cgtctagaaa aacaacagcc gtgagagcgc tactgagctc tcgccccgtg taggctgtcc
     1321 cctaaagggg cgcagcaact ggtcggaagg gccggggcga gttgcttgtc agcgcttagc
     1381 ggctccagcg cttgtccggg tcgcggtcca gcaactcgtt gcagttgcgg caggaacggg
     1441 ccttactgcc gtcggccagg aacgtatggg ccacgcgcac gggcttgttg cacttggcgc
     1501 agaccaccat caccttggag gcatccatgg gcgcctcgac ctcgacgatg ccggcctggc
     1561 gcgcgcccgc gcgcccgcgc gtgtgcttgc gcatcacgtt gatgccctcg acaatgactt
     1621 tattgtcgct ggggtggacc tcccggacgc gcccgcgctt gcccttgtct ttgcccgcga
     1681 tcaccagtac ggtgtcgtcc ttatgtacat tcattggttt ctcttattct cctggctacc
     1741 tggaacaggt gcaaaatggg cgccgcaaag gcggctaaca attataccag ctttaccggg
     1801 cgcctgtcca gcaggtcagg cgagggtcgc ttacagcacc tcaggcgcca gcgacacgat
     1861 cttcataaac tggcgctcgc ggagttcgcg ggccaccggg ccgaagatgc gcgtcccgcg
     1921 cggattgttc tgcgggttca gaatcacggc ggcgttgtcg tcgaagcgga tatgggagcc
     1981 gtcgggccgg ccatattcct tcgcggtgcg cacgacgacc gcgcgcacga cttcgccctt
     2041 cttgaccgcc ccaccgggcg ctgcctgctt cacggcggcg atgatgatat cgcccacgcc
     2101 ggcatacttc cggttcgatc cgcccatgac gcggatgcac atgatctcct tggcgccggt
     2161 gttgtcggcg acccggagcc gggtttgcgg ctggatcatg gtctagtgtc tcctctttac
     2221 ctgcgggccg gcgcttagat ctgcggcgcc cgcgacagga tctccacgac acgccagcgc
     2281 ttggtcttgc tgatcgggcg ggtctccagg atccgaacga tatcgccctc gttgcacttc
     2341 aattcgtcgt gcgccacgaa cttgttggtg cggcgcatga tcttcttgta gagccgatgg
     2401 cggacgaggc ggtcgaccgc gacgatcacg gtcttatcca ttttgttgct cacgacgcgg
     2461 ccggtgcgca tgcgcgactt gccgtgctcg ggcacccaga cgcgcgggtc ggtctcgacc
     2521 gcgccctggc cgctctggcc gggggtcggc gcgggcggca tcgccaccgc ggcttcagtc
     2581 gcggcggcag ccgtcgtcag gtcctcggcg ccgggctgat tcgtgttgtc catactgatt
     2641 agttgctcct atgaccgggg gcttgccccc gcccctatta cagcgaggcg ttcaacgcct
     2701 gtagctcggc aatctcggcg gcatacagcg cctcgatctc gcgctcccgg atgaccgtca
     2761 gcagccgggc catatcgcgg cgcaccgtct tgacgcgctg atggttcttc agcttgcgcg
     2821 tggcgatctg gaagcgcagg ttaaacagtt cgtggcgata ttcatccagg tgtcgctgga
     2881 tttccgcctc gttcatgtcg cggatttcac tcgcctgcat cgctcgtctc ctcgtccgtc
     2941 tcctcgtgcg tcacggcgcc ctcggcgatc cgtgccacga aacgggtctt gatcggcagc
     3001 ttgtgcccgg cgaggcgcat ggcctcggcg gcgacatctt cgcgcacgcc gcccagttca
     3061 aacaggatgc gcccaggccg gatcacggcc acccagtgat ccggggcgcc tttgccggag
     3121 cccatacggg tctcggcggg gcgctgggtc atcgacttgt ccgggaagat gcggatccag
     3181 accttgccgc cacgcttcac atagtgagtg atagcgcggc gggccgactc aatctggcgg
     3241 ctgtcgatcc aggcagcttc aagcgcctgg aggccatact cgccaaaggc cacggtattg
     3301 ccgcgctggg ccttaccggc gccggtggtg ccacgatgca ccttgcggtg cttcatacgc
     3361 ttgggcatta acatcggtta gctctcctcc cccggctgcg cggcctcatc ggcactcggg
     3421 gtgccggcgt cgctggcagc ggagttctcg gtggatgctt cgaggctccc ggcaccggtg
     3481 ccggcggcct cggcggtcgc gggcgcctcg ctggcgatac ccagatcggt agccggcgtg
     3541 gccgtgtcgc cctgggtctc cacggcgaca tacggggtcg caggctcctc ggcgggcgcg
     3601 gcagccggca tgctcgtttc catgctcggc ggcgcgggct gctcggcggt gggcgccgcg
     3661 ggcgcgggca gcgcctcatg gctcacctgg atcgccgtct ccgcagcggg cgcgctcgga
     3721 gtcgtctccg cggcgggcgc gggca
//
LOCUS       DASUYC010000333         3650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1195231, whole genome shotgun sequence.
ACCESSION   DASUYC010000333 DASUYC010000000
VERSION     DASUYC010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195231"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3650)
                     /locus_tag="VF276_05460"
     CDS             complement(<1..>3650)
                     /locus_tag="VF276_05460"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HEX6606348.1"
                     /translation="VYRFEVKEGLKDRYGAPVKTESWTAKVGPLPPDIDLQGGVFQPV
                     YADAPLRLEVQAPNLDQFTATLYRLTENDLRQVLNPSYNNGAPAVPGTRTRTWTVQVP
                     GGKGPAKPFWVTVGPDGAADRLPPGYWVLEATAPAPSYMGPEPLRDYVLFVSGRTALT
                     LKEENGNELVWAVDLGSGKPKPNYPVRVVRWGNGAPAGLTTGSTGADGVARLTLPANQ
                     GDDSYRTVVLSGDPNDVALVTGNWSQGIEPYSFGMSGVYSPQSESGKQRYTAAIYTDR
                     PIYRPAQTVYFRGILRLDDDARYSLPPTGAKVSVNVTAQKDGTQQIYQGQATVSPAGT
                     FDGQFTLPADAPTGQYTISFTPPNGIEATNSAGFSVEEYRKPDFQVSVQPAQPAVIAG
                     DRLNATVQTSYYFGGPVGNMTATLTLRSQPYYYYWTDPDTGETYDFNDVEWDWRIFTQ
                     PGDNSQEWTKVWQAQTGADGTLTVDMSSPITTTDHSKTVSLEAQVQDLSNQTVANRAS
                     AVIHQGAYYVGVRTEGYIATARQPMTVSLRTVAPDANAPSGGRIQPNTRVHLRYLRTE
                     WRPIPLAGAPYGVDWQREDKPVGEADVTTDGAGRATVQFSPPQAGSYRVEATSTDARG
                     HLITSSTTLWVSDSGAGSAFWQQDNPYHVNLVADKKLYKVGDTAHILVTSPYTQATGL
                     LTVERGHLRRYYVVDLRGGAPTVDVPLEAGDLPNVYIGLTMMGLGVPQTGVAEPNGPP
                     PGLTMRQGYVNLSLDTSGQKLEVSLEPQGTTFEPGSTASVVVRTRTEGGAPHPANLSL
                     AVVDEAIYALAAERGPELLSTFYGERGLEVQTATSFSGGDYGPFPTRHGYGAYDSGYG
                     AGGATSGAIPPAAAPVATAQRNATDAKTESAALDQQAPQLRTDFRDTAFWKADVTTGA
                     DGTATVQIPLPDNLTTWRLTTQGGSPDAQLLVGNAGTPITVTKPLLLRPIAPRFLTTG
                     DRAQPQAIVHNSTNSPLTVEVNLQVSGAAAYSMNVPPTRTLTVPAGQEVVATWAIEVS
                     QGTTVTLRYEARAPGGLGDALAVTVPVVPLVAPEAVATSGEVGDKVDETVFLPYGINP
                     QLGELVVQVAPSLAAGTAAGVNYVKEYPYEGTEQTVSRFLPLVTLERVYRAQGLTTPF
                     SRELPGITERAFKRLAQLQRPDGGWGWWENDPDSNAFLTAYAVQGLAAAQRAGYQFDT
                     SMLDRGKTRLFQ"
BASE COUNT          441 a         1194 c         1384 g          631 t
ORIGIN      
        1 aactggaaca ggcgcgtctt gccccggtcc agcatgctgg tgtcgaactg atagccggcg
       61 cgttgggccg ccgccagccc ctgcactgcg taggcagtca ggaaggcgtt gctgtcaggg
      121 tcgttctccc accagcccca gccgccgtcg ggccgctgga gttgggccag ccgcttgaag
      181 gcgcgctcgg tgatgccggg caactcccgg ctgaacgggg tggtcagccc ctgcgcgcgg
      241 tagacccgct ccagcgtgac cagcggcagg aaccggctca ccgtctgctc ggtgccctcg
      301 taggggtact ccttcacgta gttgaccccg gcggcggtcc cggcggccag cgacggcgcc
      361 acctgcacca cgagttcgcc cagttgcggg ttaatgccgt agggcaggaa gaccgtctcg
      421 tccaccttgt cgccgacctc gccggaggtg gccacggctt ctggcgccac cagcggcacc
      481 accggcacgg tcacggcgag ggcgtcgccc aggccgcccg gcgcgcgggc ttcgtagcgc
      541 agcgtgaccg tggtgccctg gctgacctcg atggcccagg tcgccactac ctcttgcccg
      601 gcgggcacgg tcagcgtccg cgtgggcggc acgttcatgc tgtaggccgc cgcgccgctg
      661 acctggagat tgacctcgac cgtcagcggg ctgttggtgc tgttatgcac gatggcctgc
      721 ggctgggcgc ggtcgccggt ggtcaggaag cgcggggcga tcgggcgcag cagcagcggc
      781 ttggtcacgg taatcggcgt gccggcgttg ccgacgagca gttgcgcgtc ggggctgccg
      841 ccctgcgtgg tcagccgcca ggtggtcagg ttgtcgggca gcgggatctg cacggtggcc
      901 gtgccgtcgg cgcccgtggt cacgtcggcc ttccagaagg ccgtatcgcg gaaatcggtg
      961 cgcaactgcg gcgcctgctg atcgagcgcc gcggattcgg ttttcgcgtc cgtggcatta
     1021 cgctgggcgg tcgcgaccgg cgctgccgca ggcgggatcg cgccggaagt cgctccgccc
     1081 gcgccatagc cgctgtcgta ggccccgtag ccgtggcggg tggggaaggg gccgtagtcg
     1141 ccgccggaga acgacgtggc ggtttgcact tccagcccgc gctcgccgta gaaggtggag
     1201 agcaactccg gcccgcgctc ggcggccagg gcgtagatcg cctcgtccac cacggccagc
     1261 gagaggttgg ccgggtgggg cgcgccgccc tcggtgcggg tgcgcacgac cacgctggcg
     1321 gtgctgcccg gctcgaaggt ggtgccctgc ggctccaggc tgacctccag cttctggccg
     1381 ctggtgtcca ggctcaggtt aacataaccc tggcgcatgg tcaggccggg cggcggcccg
     1441 ttgggctcgg ccacgccggt ctgcggcacg cccaggccca tcatcgtcag gccgatgtag
     1501 acgttgggca ggtcgcccgc ctccagcggc acatcgacgg tgggcgcgcc gccgcgcaga
     1561 tcgaccacat agtagcggcg caggtggccg cgctcgacgg tcagcaggcc ggtcgcctgg
     1621 gtgtagggcg acgtgaccag gatgtgcgcc gtgtcgccca ctttgtagag cttcttgtcg
     1681 gccacgaggt tgacgtggta ggggttgtcc tgctgccaga acgccgatcc ggcgccgctg
     1741 tcggagaccc agagcgtggt gctgctcgtg atcaggtggc cgcgggcgtc ggtgctggtc
     1801 gcctcgacgc gatagctgcc ggcctgcggc gggctgaact gcacggtggc ccggcccgcg
     1861 ccgtcggtcg tcacgtcggc ttcgcccacc ggcttatcct cgcgctgcca atcgacgccg
     1921 tagggcgcgc cggccagcgg gatgggccgc cactcggtgc gcaggtagcg cagatggacg
     1981 cgggtgttcg gctggatgcg cccgcccgac ggcgcgttcg cgtcgggcgc gaccgtgcgc
     2041 aggctgactg tcatcggctg gcgggccgtg gcgatgtagc cctcggtgcg cacgcccacg
     2101 tagtaggcgc cctggtggat cacggcgctc gcccggttgg cgaccgtctg gttgctcagg
     2161 tcctgcacct gggcttcgag cgacacggtt ttgctgtggt cggtggtggt gatcgggctg
     2221 ctcatgtcca cggtgagcgt gccgtcggcg ccggtctgcg cctgccagac cttggtccac
     2281 tcctggctgt tgtcgccggg ttgggtgaag atgcgccagt cccactccac gtcgttgaag
     2341 tcgtaggtct cgccggtgtc ggggtcggtc cagtagtagt agtagggctg cgagcgcagg
     2401 gtcagcgtgg ccgtcatgtt gccgacgggg ccgccgaagt agtagctggt ctgcacggtg
     2461 gcgttcagcc ggtccccggc gatgacggcg ggctgcgcgg gctgcacgct gacctggaag
     2521 tcgggcttgc ggtactcctc gacgctgaag ccggcgctgt tggtggcttc gatgccgttg
     2581 ggcggggtga agctgatcgt gtactggccg gtgggggcat ccgccggcaa ggtgaactgg
     2641 ccgtcgaacg tgcccgcggg cgacacggtc gcctggccct ggtagatctg ctgggtgccg
     2701 tccttctgtg ccgtgacatt cacgctgacc ttcgccccgg tgggtggcag gctgtagcgg
     2761 gcatcgtcgt ccagtcgcag gataccgcgg aagtagactg tctgcgcggg ccggtagatg
     2821 ggccggtcgg tgtagatcgc ggcggtgtag cgctgcttgc cgctctccga ctgcgggcta
     2881 tagacgccgg acatgccgaa gctgtagggt tcgatgccct ggctccagtt gcccgtcacc
     2941 agcgccacgt cgttcgggtc gccggagagg acgacggtgc ggtagctgtc gtcgccctgg
     3001 ttggcgggca gggtcaggcg ggccacgccg tcggcgccgg tgctgccggt ggtgaggccg
     3061 gccggtgcgc cgttgcccca gcgcaccacg cgcaccgggt agttgggctt gggtttgccg
     3121 ctgcccaggt ccaccgccca gacgagttcg ttgccgtttt cttccttgag cgtcagcgcg
     3181 gtgcgcccgg agacgaacag cacgtagtcg cgcagcggtt ccggccccat gtaggacggc
     3241 gcgggcgcgg tggcttcgag cacccagtag ccgggcggca gccggtcggc ggcgccgtcc
     3301 ggccccaccg tgacccagaa gggcttggcc ggccccttgc cgccggggac ctgcacggtc
     3361 caggtgcggg tgcgcgtgcc gggcacggcg ggcgcgccgt tgttgtagga cgggttcagc
     3421 acctgccgca gatcgttctc ggtcaggcgg tagagggtgg cggtgaactg gtccaggttg
     3481 ggcgcctgga cttccaggcg tagcggcgca tcggcgtaga ccggctggaa cacgccgccc
     3541 tgcaggtcaa tgtcgggcgg gagcggcccc actttggccg tccacgactc ggtcttgacc
     3601 ggagcgccgt agcggtcctt cagtccctct ttgacctcga agcggtagac
//
LOCUS       DASUYC010000334         2458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_968086,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000334 DASUYC010000000
VERSION     DASUYC010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968086"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..399)
                     /locus_tag="VF276_05465"
     CDS             complement(154..399)
                     /locus_tag="VF276_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606349.1"
                     /translation="MSPPAPGSATATGAATGVQDSTMAPAAGQRPAAAAQFQGHYRPG
                     RAAGARVRVLVGHPGGPDRPAPQEAGYVNKGRLRGLT"
     gene            426..>2458
                     /locus_tag="VF276_05470"
     CDS             426..>2458
                     /locus_tag="VF276_05470"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEX6606350.1"
                     /translation="MPPLPPAPLTTRYLALLAAVAALLLGVFALGPGTHAAAHPAPGV
                     GESHAPSAHPIQVYVDPQYQGSSGFIDHAGRVMTLPVGLDNAISSASLEPGWSVRLFK
                     EAYLGGTSKCLTASDPDFAGQTFEDATPLDNQVSSFYVVNDTTCPPPPPAAPGNLHVS
                     STTQGSVTLAWQDNSTNEDGFQIYRYNGSAYTRYATVPANTTTYTDINVTCNTSYSYK
                     ITAYNSTYESAQDGPAGGTTQACVVPNGVTFYLAPGYGGSYCTYAFSGPFNFTDNAQS
                     CPGFNDATASLQVQAGWAVRVYRDSNQGGPSRCYTASDPDFQNDTFSDGSPVWHQISS
                     IYVSDTGACIPVDTTPPTVHWTIPVPDNGVYTATGATVPLEVAASDASGVRDVTFTRY
                     DSATGQTITLTVVLTSPYHMTLDVGPLNYGPNRITATAADRAGNTASQSISIVKPGGT
                     PPPTVTPTATGTPVTATPTPGACTIQFTDVPVGSPFYSFIQCLVCEGIVSGYADNTFR
                     PGNDVTRGQLAKFVSNAAGYADAIPATQQTFSDVPPSHPFWLFIERAYAHGVISGYSN
                     GTFRPDTGVTRGQAAKFVANAANYTDAIPATRQTFSDVPFGNTFWLFIERVYAHGVIS
                     GYSDGTFRPGADVTRAQASRMVSAARAWSLAEPATVPFSDVQPGDWFYDYVAM"
BASE COUNT          406 a          945 c          747 g          360 t
ORIGIN      
        1 cgcccggccc atgaatgggc gggctagccc tacgaagccc gcctgcgcgg gctggcggat
       61 acatgcggcg gccggccgcg cgggagcggc accgcccggc ccatgaatgg gcgggctagc
      121 cctacgaagc ccgcctgcgc gggctggacc aaattacgtc aggccgcgca ggcggccttt
      181 gttcacgtag cccgcctctt gaggggccgg gcgatccggc ccaccgggat gccccacgag
      241 gacgcgcacc cttgcgcccg cggcgcggcc tggcctataa tgaccctgga actgcgccgc
      301 tgccgccggc cgttgcccgg cggcgggcgc catcgtggaa tcctgcaccc cggtcgcggc
      361 cccggtcgcc gtcgcggacc ccggcgccgg tggcgacacc ggcccggctc caggaaggag
      421 tccctatgcc acccctaccg ccggcccccc tcaccacgcg ctacctggcc ttgctcgccg
      481 ccgtggctgc gctgctcctc ggcgtgttcg cgctcggtcc cggcacgcac gccgccgccc
      541 atccggcgcc cggcgtggga gagagccatg cgccgtccgc gcacccgatc caggtctatg
      601 tcgatccgca ataccaggga tcaagcggtt tcatagacca tgcaggccgg gtaatgacgc
      661 tcccggtggg tctggataat gccatttcgt ctgcctcgtt ggaacccggt tggtcggtcc
      721 gcctgttcaa agaggcgtac ctgggcggga ccagcaaatg cctgaccgcc agcgacccgg
      781 acttcgccgg ccaaacgttt gaagatgcga cacccttaga caaccaggtg tcctcgttct
      841 atgtcgtgaa cgatacgact tgcccaccgc cgccgcccgc cgcgccgggc aacctgcacg
      901 tcagcagcac cacgcagggc agcgtcaccc tggcctggca ggacaattcg accaacgagg
      961 acggcttcca gatttatcgc tacaacggca gcgcctatac ccgctacgcg accgtcccgg
     1021 ccaataccac cacctacacc gacatcaacg tcacctgcaa caccagctac tcgtacaaga
     1081 tcacggccta caacagcacc tacgagtcgg cgcaggacgg ccccgccggc ggcacgacgc
     1141 aggcgtgcgt tgtcccgaac ggcgtcacct tctacctggc gccgggctac ggcggcagct
     1201 attgcaccta cgcctttagc ggcccgttta acttcacgga caacgcccaa tcctgtccgg
     1261 ggttcaacga cgccaccgca tcgctccagg tacaggccgg ctgggccgtg cgcgtctacc
     1321 gggacagcaa ccagggcggg ccgagccggt gttacaccgc cagcgacccc gatttccaga
     1381 acgacacgtt tagcgacggg tcgccggtct ggcaccagat ttcgtcgatc tatgtgtccg
     1441 atacgggcgc ctgcatcccg gtggacacca ccccgcccac ggtccactgg acgatcccgg
     1501 tgccggataa cggcgtctac acggcgacgg gggcgaccgt gccgctggag gtcgcggcct
     1561 cggacgcgtc gggcgtgcgc gacgtgacct tcacgcgcta cgactcggcc accgggcaaa
     1621 cgatcaccct gaccgtggtg ctcacctcgc cctaccacat gacactcgac gtgggcccgc
     1681 tcaactacgg cccgaaccgc atcacggcga ccgctgccga ccgggccggc aacaccgcga
     1741 gccaatcgat cagcatcgtc aagcccggcg gcacgccccc gcccaccgtc acgccgacgg
     1801 ccaccggcac cccggtcacc gcgacgccca cgccgggcgc ctgcacgatc cagttcaccg
     1861 acgtgccggt gggcagcccg ttctacagct tcattcaatg cctggtctgc gagggcatcg
     1921 tcagcggcta cgccgacaac accttccgcc ccggcaacga tgtgacgcgc ggccaactgg
     1981 cgaagtttgt gtccaacgcg gcgggctacg ccgacgccat cccggccacg cagcagacgt
     2041 ttagcgacgt gccgcccagc cacccgttct ggctgttcat cgagcgcgcc tatgcccacg
     2101 gcgtcatcag cggctacagc aacggcacct tccggcccga caccggcgtg acccgcggcc
     2161 aggcggccaa gttcgttgcc aacgccgcca attacaccga cgcgatcccg gccacccggc
     2221 agaccttcag cgatgtgccc ttcggcaaca cgttctggct gttcatcgag cgggtctacg
     2281 cccacggcgt gatcagcggc tacagcgacg gcacgttccg gcccggcgcc gatgtgaccc
     2341 gtgcccaggc ctcgcgcatg gtcagcgcgg cccgcgcctg gtccctggcc gagccggcta
     2401 ccgtgccctt cagcgatgtc cagcccggcg actggttcta cgactacgtg gcgatgat
//
LOCUS       DASUYC010000335         2160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1549094, whole genome shotgun sequence.
ACCESSION   DASUYC010000335 DASUYC010000000
VERSION     DASUYC010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1549094"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..495
                     /locus_tag="VF276_05475"
     CDS             100..495
                     /locus_tag="VF276_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606351.1"
                     /translation="MRRLALVLVVVVMLSLLGCSTAPATLTPEPFVQLTGKGNTRTLP
                     FTLGTGNYDVTWAVPSADEQGACPFSGVIAPHEPDPPLHFQAPFGNDQPATQGQITGT
                     LHLPPTLQGSYDAVITTTCVWTLTFQLVP"
     gene            704..928
                     /locus_tag="VF276_05480"
     CDS             704..928
                     /locus_tag="VF276_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606352.1"
                     /translation="MFDMTNAQRADAPVAGNLAAPSSLQDLEGFTPQEIARLTAMREQ
                     IQLRGQRDRNAEEARLRFARWRYEQGYMNG"
     gene            967..1137
                     /locus_tag="VF276_05485"
     CDS             967..1137
                     /locus_tag="VF276_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606353.1"
                     /translation="MISSLYILERQTHAETVYREAAAHQQLAHSARRHLGAPHTRVLL
                     RLGPLMIAWTTR"
     gene            1205..>2160
                     /locus_tag="VF276_05490"
     CDS             1205..>2160
                     /locus_tag="VF276_05490"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6606354.1"
                     /translation="MYDAIVVGARCAGSPTAMLLARKGYRVLVVDRATFPSDTFRNHV
                     LLYPAVRQLQRWGLLDRIAATNSPPIRLFTQDMGDFPLTGNVLETDGIDALYGPRRKY
                     LDKILVDAAVEAGAEVREGFSVQDLLRTAGRVTGIRGRTAAGTIVTEQARIVIGADGL
                     HSIVARQVGAPTYNERPVLTWAYYSYWSDVSMPGAEFYRHDDEAMLAFPTNDGLTCVA
                     AFGPIEDFSKIRLDIEGNFERMLDAFPAFTERVRGGRRMERFLGTADLPNFFRKPHGP
                     GWALVGDAGAHRDPVTGTGISEAFRDAELLADAIDAGLSGAQP"
BASE COUNT          355 a          803 c          645 g          357 t
ORIGIN      
        1 ctgcgctctc tgcgcctctg cggtgacgct aatggtaggt tgtgccgtcc ggcgaaacgt
       61 gctaggattc gctatcttcg attcgtggag gtctgttcca tgcgccgtct cgctctcgtg
      121 ctggtcgtag tagtcatgct cagcctgctc ggctgttcca cggcgcccgc cacgctgacg
      181 cccgaaccct ttgtccagtt gaccggcaag ggcaacacgc gcactttgcc cttcacgctg
      241 ggcaccggca actacgacgt gacctgggcc gtgccctccg ccgacgagca gggcgcgtgc
      301 cccttcagcg gcgtgatcgc gccgcacgag cccgacccgc cgctgcactt ccaggcgccc
      361 ttcggcaacg accagcccgc tacccagggc cagatcaccg gcacgttgca cctgccgccc
      421 accctgcaag gctcctacga cgccgtgatc accaccacct gcgtctggac gctcaccttc
      481 caactggtgc cgtaagcccg cgcaacccgc cccaaccgcc gcacgtatgg atgggtgcgg
      541 gtcgggtttt tgtcaggttc ttgtgcggat cactttccgc cggagtgtgc tttaatgata
      601 ccaacagacg ccgacgcccg ctactccgta ctcgccgctt cgccgcgttg acgcctcata
      661 gcgaaattgc ctgatatcac cgaacgaagg gatggacccg gcaatgtttg atatgacgaa
      721 cgcgcaacga gcggatgccc ctgtcgccgg caacctcgcc gcaccgagca gcctccagga
      781 tctggaggga ttcacgccgc aggaaatcgc ccgcctgacc gctatgcgcg agcagatcca
      841 actacgcggg cagcgcgacc gcaacgccga ggaagcccgg ctgcgcttcg cccgctggcg
      901 ctacgagcag ggctatatga acggatagcg ccgtcgccgg cagctatctc agccaggagg
      961 ccggccatga tcagcagcct gtacattctg gaacgccaga cacacgcgga aaccgtctat
     1021 cgagaagcag cagcccacca gcagcttgcc cactcggccc gccgccacct cggtgccccg
     1081 cacactcgcg tcctgctgcg cctcggcccc ctgatgatcg cctggaccac gcggtagccc
     1141 tcgcaccccg taacaccgcg ccccggcgcc taccgatctt ctttaccatc cagggagatt
     1201 gttaatgtac gacgccattg tcgtcggcgc gcgctgcgcc ggctcgccca ccgctatgct
     1261 tctcgcccgg aaaggctacc gggtcctggt cgtggaccgc gcgactttcc ccagcgatac
     1321 tttccgcaat cacgtcttac tctacccggc cgtgcgccag ttgcagcgtt ggggcctgct
     1381 ggatcgcatc gcggccacca atagcccgcc gatccgcctg tttacccagg acatgggcga
     1441 cttcccgctc accggcaacg tgctggagac ggacgggatc gacgcgctct atggcccgcg
     1501 ccgcaagtac ctggacaaga tcctggtgga tgccgcggtc gaagccgggg ccgaagtgcg
     1561 cgaaggcttc tcggtgcagg acctgctgcg caccgcgggc cgcgtgaccg gcatccgggg
     1621 ccgcaccgcc gcgggcacca tcgtcaccga gcaggcgcgc atcgtcatcg gcgccgacgg
     1681 cctgcactcg attgtcgctc gccaggtagg ggcgcccacc tacaacgagc ggcccgtgct
     1741 gacctgggcc tactatagct actggagcga cgtgtccatg ccgggcgccg agttctaccg
     1801 ccacgacgac gaggccatgc tggccttccc gaccaacgac ggcctgacct gtgtcgccgc
     1861 cttcggcccc atcgaagact tctccaagat ccgcctcgac attgagggca acttcgagcg
     1921 gatgctcgac gccttccccg ccttcaccga gcgtgtgcgc ggcgggcgcc gcatggagcg
     1981 cttcctgggc acggcagacc tgcccaactt cttccgcaaa ccgcacggcc ccggctgggc
     2041 gctggtcggc gacgcgggcg cgcaccgcga cccggtgacg ggcaccggca tcagcgaagc
     2101 cttccgcgac gccgaactgc tggccgacgc gatcgacgcc gggctgagcg gcgcccaacc
//
LOCUS       DASUYC010000336         2805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_177873,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000336 DASUYC010000000
VERSION     DASUYC010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177873"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..432)
                     /locus_tag="VF276_05495"
     CDS             complement(124..432)
                     /locus_tag="VF276_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606355.1"
                     /translation="MPLPIPGQKKKKQTTLEIHTLRPRLPGPWQYLGLLPMLTVARNL
                     ARQTPPAAYPLVALVVWRTIQDEQRKRRQAIFDAEREAIRRAQQIYVVKPQRKRFILF
                     "
     gene            complement(461..1462)
                     /locus_tag="VF276_05500"
     CDS             complement(461..1462)
                     /locus_tag="VF276_05500"
                     /inference="COORDINATES: protein motif:HMM:NF025380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HEX6606356.1"
                     /translation="MADDYSRGNPYGDSDDDELENLPRRPAGSDPGTARAPGASQPQA
                     WGWASDNPPPARGTPPTPPRPAAYMPDERPATRPTVTAAPPPAPAPTAPRRSTGAACI
                     WLLALAAVIVLACGLLGFGAIQSGVTGLAGWVPRLPNLPLVTPTVVIQAQGPSVVQSI
                     RSLSRIETSQYTVERVLTGESTGALPPFTSDKILFIARGEVIAGVDLQKLTEDDVQVV
                     SGTVTIRLPAPEILSSRLDNEKSRVYDRQTGIFTKADPNLESQVRQRAEEEIKSAALE
                     DGILDKARENAETTLRTLLHSLKYDNVVFVAPGGGVLGPATTPTPGELMPSDLTPSP"
     gene            1669..>2805
                     /locus_tag="VF276_05505"
     CDS             1669..>2805
                     /locus_tag="VF276_05505"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX6606357.1"
                     /translation="MDRDPPDNRDPRQPPRIPRWTDPTWAPEDFPQSPGFDPSVFDSE
                     ETRPMPTGDRAPRPPAPQPPPPPPATPPPAPPRRNARRLSESELPPPVREPGSPRRGA
                     GRLNESDLPTDIRGAPPPRRGAGRLSEDDLPPDVPGAAPPAAEWGAFPPDPGAPSPPA
                     PRWGGAQPPPGEPPPPGEAAPAPRVSRSAAASPSAPAPRAPRSGATRSRQRSIAPPAG
                     RKWVQRRRIILAALALVAIGLCAWLGPLFFKGQALISDIFVTPPPRPSGVSAYPDWNK
                     QERVNILLMGLDTRQPGDSRSDTLILVSIDPAHKTVAMLSIPRDLWVPIPGFGENRIN
                     AAYQYGETNNVPGGGPALVMQTVEQDFAVPVHYFAQVDFNGFRRI"
BASE COUNT          378 a         1014 c          981 g          432 t
ORIGIN      
        1 gttgtggtca tgtcatagct cctttagtcc cgctcgccgg cgggcacgga atggcggcat
       61 caggatggat cgccgtttcc ctacggtcac ggcaacatat atgccaccag ggcgccgctc
      121 cggctaaaag aggatgaacc gtttgcgctg cggcttgacc acgtagatct gctgggcgcg
      181 gcggatcgct tctcgctcgg cgtcgaagat ggcctgccgg cgcttgcgct gctcgtcctg
      241 gatggtgcgc cagacgacca gggcgaccag gggataggcg gcggggggcg tctggcgggc
      301 caggttgcgc gccaccgtca gcatgggcag caggcccagg tattgccagg ggccgggcag
      361 ccgcgggcgc aaggtatgga tctccagcgt cgtctgcttc ttcttcttct gaccgggaat
      421 gggtaacggc atggctctcc tccgcgacac gaaccgctgt ctacgggcta ggggtcaaat
      481 ccgagggcat caactcgccg ggtgtgggag tcgtggccgg gcccagcacg ccgccgccgg
      541 gcgccacgaa caccacgttg tcatacttga gactgtgcag cagcgtgcgc agcgtcgtct
      601 cggcgttctc gcgggccttg tcgaggatgc cgtcctccag cgccgcgctc ttgatttctt
      661 cctcggcgcg ctggcgcacc tgcgactcca ggttcgggtc ggccttggtg aagatgcccg
      721 tctgccgatc atagacccgc gacttctcgt tgtccaggcg gctgctgagg atctccggcg
      781 cgggcaggcg gatggtgacg gtgccgctga ccacctgcac gtcgtcctcg gtcagcttct
      841 gcaagtccac gcccgcgatg acctcgcccc gcgcgatgaa caggatcttg tcgctggtga
      901 agggcggcag cgccccggtc gactcgccgg tcagcacgcg ctcaacggtg tactgcgagg
      961 tctcgatgcg gctgagcgag cggatggact ggaccaccga tgggccctgg gcctggatca
     1021 ccacggtcgg cgtgaccagc ggcaggttgg gcaggcgcgg cacccacccg gccaggccgg
     1081 tgacgccgct ctggatggcg ccaaagccga gcaggccgca ggccagcaca atcaccgcgg
     1141 ccagggccaa cagccagata caggcggcgc cggtgctccg gcgcggcgcg gtgggcgcgg
     1201 gggcaggggg cggcgccgcc gtcacggtgg gtcgggttgc gggccgctcg tccggcatat
     1261 aggctgcggg gcgcggcggt gttggcggcg tgccgcgcgc cgggggcgga ttgtcactcg
     1321 cccagcccca tgcctgcggt tggctagcgc ccggcgcgcg tgctgtgccg gggtcggacc
     1381 ccgccgggcg gcggggcagg ttttcgagtt cgtcgtcgtc gctatcgccg tacggattcc
     1441 cgcggctgta atcgtcggcc atgctgcctg cctctctggg cggaccggag tccgcgaatc
     1501 atcgccagtg tataacggca cccatcgcgg cgccgtgcgt cttacctcta ttataagcac
     1561 acggcgtacc ggcgaatcgg cccaccgggc gggtaggatc gcgcgcgggt ttgtgctata
     1621 atatctgcgc tctacataat actacggcaa acgccaggag ctgtctactt ggaccgcgac
     1681 ccgcccgaca accgagaccc ccgccagccg ccgcggattc cccgctggac cgacccgacc
     1741 tgggcgccgg aggactttcc gcagtcgccc ggcttcgacc cctcggtgtt cgactcggag
     1801 gagacgcggc ccatgcccac gggggaccgg gcgccgcggc cccccgcgcc gcaacccccg
     1861 ccgccgccac ccgcgacccc cccgcccgcg cccccgcgcc gcaacgcccg ccgcctgagc
     1921 gagtcggagt tgccgccgcc ggtgcgcgag ccgggctcgc cacggcgggg cgccgggcgg
     1981 ttgaacgaat cggacctgcc aactgatata cgcggggccc cgccgccacg tcgcggggcc
     2041 gggcgcctga gcgaggacga tttgccgccc gatgtgccgg gcgcggcccc gcccgcggcg
     2101 gagtggggcg ccttcccgcc ggacccaggc gccccgtcgc ctcccgcgcc gcgctggggt
     2161 ggcgcgcagc cgccgcccgg cgaaccgccg ccgcctggcg aagcggcgcc cgccccgcgg
     2221 gtgtcgcgca gcgccgccgc atctccgtcc gcccccgcgc cgcgcgcgcc gcgttcgggg
     2281 gccacccggt cacgccagcg ctctatcgcg ccgcctgccg ggcggaaatg ggtgcagcgc
     2341 cgccgcatca tcctggccgc cctggccctg gtcgccatcg ggctgtgcgc ctggctgggc
     2401 ccgctgttct tcaagggcca ggcgttgatc agcgatatct tcgtcacgcc cccgccgcgc
     2461 ccctccggcg tgtcggccta tcccgactgg aacaagcagg agcgagtcaa tatcctcttg
     2521 atggggctgg acacccgcca accaggcgac tcgcgcagcg acacgctgat cctggtcagc
     2581 attgacccgg cgcacaagac cgtggcgatg ctctcgatcc cgcgcgacct ctgggtgccc
     2641 atccccggct tcggcgagaa ccgcatcaac gcggcctacc agtacggcga gacgaacaac
     2701 gtgcccggcg gtggcccggc cctggtcatg cagacggtgg agcaggactt cgccgtgccg
     2761 gtgcattact tcgcccaggt cgactttaac ggcttccggc gcatc
//
LOCUS       DASUYC010000337         3583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_515175,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000337 DASUYC010000000
VERSION     DASUYC010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515175"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VF276_05510"
     CDS             complement(<1..371)
                     /locus_tag="VF276_05510"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606358.1"
                     /translation="MALLTEWKTLEYRPTWGPSVAWSPDSRVLACGSGEHTIRLWDVA
                     TGQPIQTFTGPYCYSYFLVYSPDGITLASGDEEFDPAVVRLWDVPSGRLRLTLPGQAD
                     FITDLAFSPDGTLLASAGDGVR"
     gene            complement(459..899)
                     /locus_tag="VF276_05515"
     CDS             complement(459..899)
                     /locus_tag="VF276_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606359.1"
                     /translation="MGRIWVAWVMLGFAGLGGMLSLLLLVATFLYAPSNVIALRDWTL
                     WIMFGLFFPTTLLLAFYHLLFSARRRAGVTIIPMSRRLRLSLALLVLGYGVIFIYLTV
                     STANSTDELVKQVYIIRSWLAMTCFAGASLILVTLRTAFGAGNK"
     gene            complement(981..1199)
                     /locus_tag="VF276_05520"
     CDS             complement(981..1199)
                     /locus_tag="VF276_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606360.1"
                     /translation="MKPVRDVWQAIRTRRAGRYCFGMGLGWLLAALLIVLTDAWIGAN
                     WLNSNAPGFIVVGLAWLLIALILVWRDR"
     gene            1568..1840
                     /locus_tag="VF276_05525"
     CDS             1568..1840
                     /locus_tag="VF276_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606361.1"
                     /translation="MGPADRPPAVAVPAEAAPWQPALDRLGLGSSAALVTDLVRPVSW
                     LGAQLLLILQPTLTLLGAGPRVDHLTDYLETLDSRPTGAPGEEDVC"
     gene            1834..2670
                     /locus_tag="VF276_05530"
     CDS             1834..2670
                     /locus_tag="VF276_05530"
                     /inference="COORDINATES: protein motif:HMM:NF042826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6754 domain-containing protein"
                     /protein_id="HEX6606362.1"
                     /translation="MLIATGLALLILLFFILALVLMTPRIRRGQRVTLRPLRAYDAAH
                     HAIARTAEQGRTTHLSTGPGALAGDTPRTAETLAGLEVMHQMARRAAATGTPLLATTG
                     SGLAFPMAENVVQAGYAEVGRLDEVPLVGGASTAAQAVTLEGRAQGVRMLTHHDPMAY
                     AAAAGALAENEGVSNGVLVGGWSAEYLLVNEAQARAGVHAVAGSADPQGLATQLLTAD
                     QTLIGEEIYTAGAYLDARPAHQASLIAEDTIRVLLILLIVVGALLATAGINLAPLLGV
                     SR"
     gene            2667..3368
                     /locus_tag="VF276_05535"
     CDS             2667..3368
                     /locus_tag="VF276_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606363.1"
                     /translation="MRGRRQANRAPRAGAPVDRPPRPRRQVNLFIALNILISLLVGGL
                     VLFGFFAPGAGGVLVQLFSTWTTTIIVFALLLGILNLLRVHLTRIARRGAGWPYSLAL
                     VLSAALVLILGFSGTWSAGDPAVQWVFDWVYSPIASSLFALLAFFVITAAFRALRAGP
                     SAAWGMLAVAVLVIVGTAPWSREGPLSLLADTKDWIVNYPALAGLRGILLGAALGAVA
                     TSLRVLLGIDRPYVS"
     gene            3380..>3583
                     /locus_tag="VF276_05540"
     CDS             3380..>3583
                     /locus_tag="VF276_05540"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEX6606364.1"
                     /translation="MYCARCGESNPAGSAYCAKCGSPVGPAPGKGTPNGRRCPMCGVV
                     NRADALYCANCGARVVPVDELSED"
BASE COUNT          620 a         1239 c         1165 g          559 t
ORIGIN      
        1 cgcacgccgt cgccggcgct cgccagcagc gtgccgtccg ggctgaaggc gagatcggtg
       61 atgaagtcgg cctgaccggg cagggtcagc cgcaaccgcc cgctgggcac atcccagagc
      121 cgcaccaccg cgggatcgaa ctcctcatcc ccgcttgcca gcgtgatgcc gtcgggactg
      181 tagaccagga agtaggaata gcaatacggt ccggtgaagg tctggatcgg ctgcccggtc
      241 gccacatccc agagacggat ggtgtgttcg ccgctgccgc aggccagcac ccggctatcg
      301 ggactccagg ctacggacgg tccccaggtg gggcgatact ccagcgtctt ccactccgtc
      361 agcagcgcca cgactgcatc ctcctacatc atccgctctt gatctatcgt gagcataatc
      421 cggaaggcac ctgtgaagcc ctgctagccg ccttacaact atttgttccc tgccccgaac
      481 gcagttctga gggtaacaag gatcaaactc gcaccggcaa agcaggtcat agccagccaa
      541 gaccgaataa tatagacctg cttcactagc tcgtcagtgg aattagcggt ggatacggtg
      601 aggtagataa agataacgcc gtagcctaat accaggagcg cgagcgacaa ccgcaaccgg
      661 cgtgacattg gtattatcgt tacaccggcc cgcctgcgtg cgctgaacag cagatggtaa
      721 aatgccagca acagcgttgt agggaaaaag agaccgaaca taatccacaa cgtccaatcg
      781 cgcaaggcga tgacattgga cggcgcatat aagaatgtgg caacaagcag cagtagactg
      841 agcataccgc ccagaccagc aaacccgagc atcacccacg ctacccagat tcttcccatc
      901 tcctcatttc cccgctagcc cggcgccgcg ccgatacacc gtggcagcgg cacaggccag
      961 tgccatcggt attgctgcca tcatcgatct cgccaaacca ggataagagc tatcaatagc
     1021 caggccagcc cgaccacgat aaaccctgga gcgttgctgt tgagccagtt cgccccgatc
     1081 caggcgtcgg tcagcacgat cagcagcgcc gccaacagcc agcctaagcc catcccgaag
     1141 caatagcgac cggcgcggcg ggtacggata gcttgccata cgtcacggac aggtttcatc
     1201 gtcgttccgc ctttaccttc cctcggcgca gtccatggta tttgatggaa aagctagcga
     1261 gtgggaatac ggggtgctac caaccggccc caacaacgcc attctaacac gctcagtcag
     1321 cgctttcaat actaacagaa gccccgatag ctggcggcgg ggcgcgggga tcgcgggcgc
     1381 acacccaaag ccgccgggcg caggcgggcg caggtggccg gatacttgac gtaacataac
     1441 gcctgtacta taatggccct aatccaacgg ccccctctgc cggcgatatc gtgacggacg
     1501 gcggcgcaga gaggctagag agccgccaat ggccccagac acaacaggca agcgtgatca
     1561 aggaccggtg ggtcccgcgg atcgcccgcc cgccgtggcg gtcccggcgg aggccgcgcc
     1621 ctggcagccc gcgctggatc ggctggggct aggctccagc gcagccctgg tcacggacct
     1681 ggtgcgcccc gtgtcctggt tgggtgccca actgctgctg atcctccaac ccacgctcac
     1741 cctcctcggc gccggcccgc gggtggacca cctgacggac tacctggaaa ccctcgactc
     1801 gcgcccgacc ggcgcgcccg gagaggagga cgtatgctga tcgccaccgg gctggcgctg
     1861 ctgatcctgc tgttcttcat cctcgccctc gtgctgatga ccccgcgcat ccggcgcggc
     1921 cagcgcgtga cattgcgccc gctgcgcgcg tatgatgccg cgcaccacgc catcgcccgc
     1981 acggccgagc agggccgcac cacccacctg tccaccggcc ccggcgcgct ggccggcgac
     2041 acgccgcgca ccgccgagac gctggccggg ctggaggtca tgcaccagat ggcccgccgc
     2101 gccgccgcca cgggcacgcc gttgctggcg acgaccggca gcgggctggc tttccccatg
     2161 gccgagaacg tggtgcaggc cggctacgcc gaagtgggcc gcctggacga agtgccgctg
     2221 gtcggcgggg cctcgaccgc ggcccaggcc gtgacgctgg agggccgcgc ccagggcgtg
     2281 cgcatgctca cccaccacga cccgatggcc tacgccgccg cggcgggtgc gctcgccgag
     2341 aacgaagggg tgagcaacgg cgtgctggtc ggcgggtgga gcgcggagta tctgctggtg
     2401 aacgaggccc aggcccgcgc gggcgtccac gccgtggccg ggtccgccga cccgcagggg
     2461 ctggcgacgc aactgctgac cgccgaccag acgctgatcg gcgaggagat ctacacggcg
     2521 ggcgcgtatc tggacgcgcg gcccgcgcac caggccagcc tgatcgccga ggacacgatc
     2581 cgcgtgctgc tgattctgct gattgtggtg ggcgcgctgc tggcgacggc cggcatcaat
     2641 ctcgcgccgt tattgggggt tagtcgatga gaggacgccg ccaggcgaac cgcgcgcccc
     2701 gcgccggcgc gcccgtggac cggccgccgc ggccccgccg ccaggtcaat ctctttatcg
     2761 ccctgaacat cctcatcagc ctgctggtcg gcgggctggt gttgttcggc tttttcgcgc
     2821 cgggcgcggg cggggtgctg gtccagcttt tcagcacgtg gaccaccacc atcatcgtct
     2881 tcgccctgct gctgggcatt ctcaacctgc tgcgcgtcca cctgacgcgc atcgcgcggc
     2941 ggggcgcggg ctggccctac agcctggcgc tggtgctgag cgcggcgctg gtgctgatcc
     3001 tgggcttctc cggcacctgg agcgccggcg accccgccgt gcagtgggtc ttcgactggg
     3061 tctacagccc catcgcgagc agcctcttcg cgctgctggc gttcttcgtc atcaccgccg
     3121 ccttccgcgc cctgcgtgcc gggccgagcg cggcctgggg catgctggcg gtggccgtgc
     3181 tggtgatcgt cggcacggcg ccctggagcc gtgaaggccc gctgagcctg ctggccgaca
     3241 cgaaggactg gatcgtcaat tacccggcgc tggccgggct gcgcggcatc ttgctgggcg
     3301 cggccctggg cgcggtggca acaagtctgc gggtgctgct cggtatcgat cgcccgtatg
     3361 tgagctgagg aactgaggaa tgtactgcgc ccgttgcgga gaatcgaatc cggccggcag
     3421 cgcctactgc gccaagtgcg gtagccccgt tggccccgcg cccggcaagg gcaccccgaa
     3481 cggccggcgc tgccccatgt gcggggtagt caaccgcgcc gatgccctct attgcgccaa
     3541 ctgcggcgcg cgggtggtgc cggtcgacga actaagcgag gac
//
LOCUS       DASUYC010000338         6280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_241160,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000338 DASUYC010000000
VERSION     DASUYC010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6280
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241160"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..214
                     /locus_tag="VF276_05545"
     tRNA            143..214
                     /locus_tag="VF276_05545"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:175..177,aa:Met,seq:cat)
     gene            294..938
                     /locus_tag="VF276_05550"
     CDS             294..938
                     /locus_tag="VF276_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HEX6606365.1"
                     /translation="MYLPPAFREDRLEVQHELIRAHPLGLLITAGPCGLVANPVPMLI
                     YPDEGELGTLRAHLSRANRQWEEIAAVGECLVVFQGPQDYISPSWYASKQETGKVVPT
                     WNYCTVHVWGRPQLMEDAGWLRRQLKDLTQAQEGARPRPWRVDDAPESYIAGQMKGIV
                     GVEIAITRSEGKWKLSQNRSQADRSGVVAGLRQDGAAHEPMAALVAERLHSGGR"
     gene            complement(1006..2028)
                     /locus_tag="VF276_05555"
     CDS             complement(1006..2028)
                     /locus_tag="VF276_05555"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6606366.1"
                     /translation="MADPIAPTQPAPGSAVHDGSQDGRGDPAPDAAAQVERSEALAAP
                     TKVHVKFWGTRGSIPVSGDSYRRYGGNTVCVSVTSDTGHVFIFDAGSGIRPLGIDLAQ
                     AATAPGGTPPTGYLLLTHTHWDHIQGFPFFRPVFTPQARFGIIGCSDRTQKLVELLAG
                     QMDWSYFPVALASLPAHLTFHAMCGGECLLDGAKLTMFLQKHTLPSLAFRVQLGEKSV
                     VYATDNEPLSPPAPAADALLGYDVIDERMVEFARGADLLIHDAQYTLEEYASHIGWGH
                     NVPEVAVDAAIRAGVKCLALFHHDPDHNDDALDAMLARARARAERLAPGALQVISARD
                     GMEFPL"
     gene            complement(2169..2495)
                     /locus_tag="VF276_05560"
     CDS             complement(2169..2495)
                     /locus_tag="VF276_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606367.1"
                     /translation="MPADRPLRNLTRDHLLGVYTELQSEPTSKKYNLRKELQGDKRDN
                     VVYANRWLTRDQYLEMARQEILARGLDLPAEEPAEPPAAVASAAPEAAPAAPATADTT
                     PAATPE"
     gene            2773..3417
                     /locus_tag="VF276_05565"
     CDS             2773..3417
                     /locus_tag="VF276_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6606368.1"
                     /translation="MNLADEWITLAEAAALLATPLSTVRRWGDQGILQVRRTPGGHRR
                     FSRASVVAARDRAPGATPPVLPAPAPWNLDQTVLARQNWHAHFQAAGEAGRMRGLGQR
                     LLGLLIQYLTRREEDSRFLVEGREIGTAYGTEAAQSAASLHDLVEAFLFFRSSFMALA
                     LQVPAAARANDAGEIARLWQRADRFMNEVLLGTIRGYEAARSTEQPGELDRPWT"
     gene            3408..4649
                     /gene="hemA"
                     /locus_tag="VF276_05570"
     CDS             3408..4649
                     /gene="hemA"
                     /locus_tag="VF276_05570"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEX6606369.1"
                     /translation="MDLIVTGLSHQTAPLEEREAATLRGEKLPDALARLARVAGEGAI
                     LSTCNRTEIYAYSDGDPAELAAMLRAALLGPAGSGDHWYTRSGADAAAHLFRVAAGLD
                     SLVPGEVQILGQIKEAWAAAHAAGVAGPVCSALFQRALAAGKRVRSTTAISALPASIS
                     YAAVSLVRRILGGLEGRRVLVVGAGAMGEAVARCLMENGAGALLVANRHAARAQALAA
                     AHGGSAVAWAELPAALAAADVVISATDAPGFVLPLDLVRQAAHARPETPLHLIDLAVP
                     RDIDPACARLPGVVLHNIDDLRDVVDAGLAARRAALPAAAAIVEEEAAAFCAWLESRR
                     AAPTIEALHRRADAIRQNETEWALSHLNDLSARDRQIVEALAARLVGKLLHEPTVRLR
                     ELAGAPEGDTYTAAIHTLFDL"
     gene            4665..5363
                     /locus_tag="VF276_05575"
     CDS             4665..5363
                     /locus_tag="VF276_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HEX6606370.1"
                     /translation="MEPTGETTPANQPMFVSYTFFKTDPAWRRLPAGERDRGRAEFRT
                     AVDRAGGVTTYAYSTVGLKADADLLLWRSSPTLDALQESMAALLQTGLGSYTTITRTM
                     FGMTRPSHYTKRRTVQEQAVGLKERERYLVVYPFVKTIGWYLMSQEARQGMMNEHIRV
                     GHEFPGVRQVLLYSTGLDDQEFIVVYEMADLAEFQALVIALRDTEARRYTERDTPIAT
                     AIHRPLDEALALLG"
     gene            5373..>6280
                     /gene="hemE"
                     /locus_tag="VF276_05580"
     CDS             5373..>6280
                     /gene="hemE"
                     /locus_tag="VF276_05580"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020542919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEX6606371.1"
                     /translation="MSESAARFLRACRRQEVDTTPVWFMRQAGRSLPEYRAIRERYSL
                     LEICRRPEVCAEVTLQPIRRLGVDAAILFADIMIPLIATGVDLDIVENVGPVIRHPLR
                     TAGDIAALRPIEPEQDVPFVLETIRLLRGELGGTPLIGFAGAPFTLAAYLVEGKPSRD
                     FVRVKGFMYREPNLWHDLMTRLSDMVAAYLQAQIVAGAQAVQLFDSWVGALSPRDYAR
                     YVLPYSRRILSAVGGGVPVIHFGTNSATLLDQMAAAGGDVIGVDWRIPLDAAWERVGP
                     DRGVQGNLDPCVLLAPPAVIEEQARDV"
BASE COUNT          986 a         2107 c         2164 g         1023 t
ORIGIN      
        1 tcacgaccat atctgagcat agtgcgggcc aacgcgggat tctgccctgt tttgcggctc
       61 aggtcgcttg acagggcggg cggcatcccg tataatgatg cacgtcaaca gagccggagt
      121 tcgctcccgc tctgtcgttt atggcggggt agctcagtgg tagagcaggg gactcataag
      181 cccttggtcg agagttcaaa tctctccccc gccacccagt gcccacgcaa gcagagagcc
      241 gccctcaccg gggcggcttt tgttataccg cccgtaaaga ggaggagtga acaatgtacc
      301 tgccccctgc atttcgcgag gatcggctgg aggtgcaaca cgaactgatc cgggcgcacc
      361 cgctgggcct gctcatcacg gccgggccgt gcggactggt ggcaaacccg gtgcctatgc
      421 tgatctatcc tgatgaaggt gaacttggta ccctgcgcgc gcatctctcg cgggccaacc
      481 ggcagtggga agagatcgcc gccgtggggg aatgcctggt ggtgttccag gggccgcaag
      541 actatatcag tccatcctgg tacgccagta agcaggagac gggcaaggtc gtgcccacct
      601 ggaactactg caccgtccat gtctggggcc ggccgcagct aatggaagat gccggctggt
      661 tgcggcggca actgaaggac ctgacgcagg cgcaggaagg cgcccgtccg cggccctggc
      721 gcgtggacga cgcaccggag agctacatcg ccgggcaaat gaagggcatc gtgggcgtgg
      781 agatcgccat aacgcgcagt gaaggcaaat ggaagctgag ccagaaccgc tcgcaggccg
      841 atcgcagcgg ggtggtggcg ggcctgcgcc aggacggcgc ggcgcacgaa ccgatggccg
      901 ccctcgtcgc tgagcggctg cactcgggag gccgatagag acggtcggta gcgttgcccg
      961 gcgcatccct ggccggcagg cggcaggcgg tttttcagcg cacgcttaaa gcggaaactc
     1021 catgccgtcg cgcgcgctga tgacttgcag cgcgccgggg gccaggcgct cggcgcgtgc
     1081 gcgggcgcgg gccagcatcg cgtcgagcgc gtcgtcgttg tggtccgggt cgtggtggaa
     1141 cagcgccagg cacttgaccc cggcccggat ggcggcatcc acggccactt ccggcacatt
     1201 gtggccccag ccaatgtgcg acgcatactc ctccagcgtg tattgcgcgt cgtggatcag
     1261 taaatcggcg ccccgcgcga attccaccat gcgctcgtcg atcacgtcgt agcccagcag
     1321 ggcgtccgcc gccggggccg ggggactcag cggctcgttg tcggtggcgt agactacgct
     1381 cttctcgcca agttgcacgc ggaaggccag gctgggcagg gtgtgcttct gcaggaacat
     1441 ggtcagcttc gcgccgtcca gcaggcactc gcccccgcac atcgcgtgga aggtgaggtg
     1501 cgcgggcaga ctcgccagcg ccacggggaa atagctccaa tccatctgcc cggccagcag
     1561 ttcgaccagc ttttgtgtgc ggtcgctaca gccgatgatg ccgaagcggg cttgcggggt
     1621 gaagaccggg cggaagaagg ggaagccctg gatatggtcc cagtgggtgt gcgtcagcag
     1681 tagatagccg gtgggcgggg tgccgccggg ggccgttgcg gcctgggcca gatcgatgcc
     1741 caggggacgg atgcccgacc cggcgtcgaa aatgaacacg tggccggtgt cgctggtgac
     1801 gctgacacag acggtgttgc cgccgtagcg ccggtagctg tcgccggaaa cgggaattga
     1861 gccgcgggtg ccccagaatt taacgtggac cttggtgggc gccgcgaggg cttcgctgcg
     1921 ctccacttgc gcggccgcgt cgggggcggg atcgccgcgt ccatcctgcg agccgtcatg
     1981 aaccgccgag ccgggtgccg gttgtgtggg cgcgatcgga tctgccatag gtaccgattg
     2041 ctcctttgtt accccaaact cgccgggcca atccggcggc actgcctgcg ctggtcgcgt
     2101 gctggtcagg tctagtatac acacgaaccg ccgggctatg caatagtatt accgccgcgg
     2161 gccggaggtt actccggcgt cgcggcgggc gtggtgtccg ctgtcgcggg tgcggcgggc
     2221 gcggcctccg gtgcggcgga tgcgacggcg gccggtggct ccgcgggttc ttcggcgggc
     2281 agatccagcc cgcgcgcgag gatctcctgg cgggccatct ccaggtactg gtcgcgggtc
     2341 agccagcggt tggcgtacac cacgttgtcg cgcttatccc cttgcaactc tttccgcaaa
     2401 ttgtacttct tgctggtcgg ctcgctttgc agctccgtgt agacacctaa caggtggtca
     2461 cgggtcaggt tccgtaaagg gcggtcggct ggcatactgg cgctccttga cataccgccc
     2521 ggcgcacaac gtgcggacgc ggcgggagtg tattgcatca ctatagccga aaacatgcca
     2581 accggcaagg atcgatcccg gcagatatat accatcataa gaatacctta attttgcgta
     2641 taaaatgccg aatctctctt gacgcccatc catctacgac atatactcag agtaaggaaa
     2701 tctacagcag ttttgagaga tttgatagat tccatggata acagtgcggc tggaaagcac
     2761 gatattgata atatgaacct cgctgacgag tggattaccc tcgcggaggc cgctgcgctc
     2821 cttgcgacgc cgctgtccac cgtgcgccgc tggggcgacc agggcatctt gcaggtgcgc
     2881 cggacgccgg gcggccatcg ccggttctcg cgcgcctccg ttgtcgcggc gcgcgaccgg
     2941 gcgccgggag ccacgccgcc ggtgctgccc gcgcccgcac cctggaacct ggaccagacc
     3001 gtgctggccc gccagaactg gcacgcccac ttccaggctg ccggggaggc ggggcgcatg
     3061 cgcggcctcg gccagcggtt gctggggctg ctcatccagt acctgacccg ccgggaagaa
     3121 gatagccggt tcctggtcga aggccgcgag atcggcaccg cctacggcac cgaggcggcc
     3181 cagtccgctg cgagcctgca cgacctggtc gaggcgtttc tgtttttccg cagcagcttc
     3241 atggccctgg cgctccaggt gcccgccgcg gcccgtgcca acgacgcggg cgagattgcc
     3301 cggctgtggc agcgcgccga ccgctttatg aacgaggtgt tgctgggcac gatccgcggc
     3361 tatgaagccg cgcgatccac cgagcaaccg ggagaactcg accgtccatg gacctgattg
     3421 tgaccgggct gagccaccag acggctccgc tggaggaacg cgaggcggcg actctgcgcg
     3481 gcgagaagtt gcccgacgcc ctggctcggc tggcccgcgt ggcgggggag ggcgcgatcc
     3541 tctccacttg caaccgcacc gagatctacg cctatagcga cggcgatccg gcggaactgg
     3601 ccgccatgct ccgcgccgcg ttgctcggcc ctgccggatc gggcgaccac tggtatacgc
     3661 gcagcggggc ggatgccgcc gcacacctgt ttcgcgtggc ggcgggactg gattcgctgg
     3721 tgcccggcga ggtgcagatc ctgggccaga tcaaggaggc gtgggcggcc gcccatgccg
     3781 ccggggtcgc tggaccggtc tgctcggcgt tgttccagcg cgccctggcc gcgggtaagc
     3841 gcgtgcgcag cacgaccgcc atcagcgcgc tcccggcctc gatcagttat gccgccgtga
     3901 gcctggtccg ccgcatcctg ggtgggctgg agggtcgccg ggtgctggtt gtgggcgcgg
     3961 gggccatggg cgaggcggtg gcgcgctgcc tgatggagaa cggcgccggg gcgctgctgg
     4021 tcgccaaccg ccatgccgcg cgggcgcagg ccctggccgc cgcccacggc ggtagcgccg
     4081 tcgcctgggc tgaactcccc gccgcgctgg ccgccgccga tgtggtgatc agcgccaccg
     4141 atgcccccgg cttcgtgctg cccctggatc tggtccggca ggcggcgcac gcgcgacccg
     4201 agaccccgct gcatctcatc gacctggccg tgccccgcga catcgatccg gcgtgcgcgc
     4261 ggctgccggg cgtggttctg cacaatatcg acgatctgcg cgacgtggta gatgccgggc
     4321 tggcggcccg gcgcgcggcg ctgcccgccg cggcggccat cgtcgaggag gaagccgccg
     4381 ccttttgcgc ctggctggag tcgcgccgcg ccgcaccgac catcgaggcg ctgcaccgcc
     4441 gcgccgacgc gatccgccag aacgaaaccg agtgggcgct gtcccatctg aacgacctct
     4501 cggcgcgcga ccggcagatc gtcgaggcgc tggccgcccg cctcgtcggc aagctgctgc
     4561 acgagccgac cgtgcgcctg cgcgaactgg ccggcgcacc cgaaggcgac acctataccg
     4621 cggccatcca tacgttgttc gatctctagt caggagcctg ttccatggag ccaaccggcg
     4681 aaaccacccc ggcgaaccag ccgatgtttg tcagctatac cttcttcaag accgacccgg
     4741 cctggcgccg tttgcccgcc ggcgaacgcg accggggccg cgccgagttc cggaccgccg
     4801 tggatcgcgc cggcggggtg acgacctacg cctacagcac cgtcggcctc aaggccgatg
     4861 ccgacctctt gctctggcgc agcagcccca cgctggacgc gctgcaagag agcatggccg
     4921 ctttgctaca gaccggcctc ggcagctaca cgacgatcac gcggaccatg ttcggcatga
     4981 cccggccctc gcactacacc aaacggcgca cggtgcagga gcaggccgtg ggcctcaagg
     5041 agcgtgagcg ctacctggtg gtctacccgt tcgtcaagac cattggctgg tacttgatga
     5101 gccaggaggc ccgccagggc atgatgaacg agcatatccg cgtgggccat gagttccccg
     5161 gcgtgcgcca ggtgctgctc tattccaccg gcctggacga ccaggagttc atcgtggtct
     5221 acgagatggc cgacctggcc gagttccagg ccctggtcat cgccctgcgc gacaccgagg
     5281 cgcgccgcta caccgagcgc gacacgccca tcgccaccgc gatccatcgc ccgctggacg
     5341 aagcgctggc gctgctagga tagaggccgc ccgtgagcga atccgccgcc cgcttcctgc
     5401 gcgcctgccg ccgccaggag gtggatacca cgcccgtctg gttcatgcgc caggccgggc
     5461 gcagcctgcc cgaataccgc gcgatccgcg agcgctattc gctgctggag atctgccgcc
     5521 gcccggaggt ctgcgccgaa gtgacgctgc aaccgatccg ccggctcggc gtggacgccg
     5581 ccatcctctt cgccgacatc atgatcccgc ttatcgccac tggggtcgat ctcgacatcg
     5641 tggagaacgt ggggccggtg atccgccacc cgctccgcac ggcaggggac atcgccgcgc
     5701 tgcgccctat cgagcccgag caagacgtgc cctttgtgct ggagacgatc cgcctgctgc
     5761 gcggggaact cggcggcacg ccgctgatcg gcttcgccgg ggcgccgttc acgctcgccg
     5821 cctacctggt cgaaggcaag ccctcgcgcg acttcgtccg cgtcaagggc ttcatgtacc
     5881 gcgaacccaa cctctggcac gacctgatga cccggctcag cgacatggtc gccgcctatc
     5941 tccaggccca gatcgtggca ggcgcacagg cggtgcaact gttcgatagc tgggtgggcg
     6001 ccctcagtcc ccgcgactac gcgcgctacg tgctgcccta ttcgcggcgc atcctgagcg
     6061 cagttggcgg tggcgtgccg gtgatccact tcggcactaa cagcgcgacc ctgcttgacc
     6121 agatggcggc ggcgggcggc gacgtgatcg gcgtcgattg gcgcatcccg ctggacgcgg
     6181 cctgggagcg ggtggggccg gatcgggggg tacagggcaa cctcgacccc tgcgtgctgc
     6241 tggccccgcc cgccgtgatc gaggaacagg cccgcgacgt
//
LOCUS       DASUYC010000339         2326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_479308,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000339 DASUYC010000000
VERSION     DASUYC010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479308"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /locus_tag="VF276_05585"
     CDS             complement(<1..1098)
                     /locus_tag="VF276_05585"
                     /inference="COORDINATES: protein motif:HMM:NF013570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HEX6606372.1"
                     /translation="MDAQTLRRAFLDYYQARGHAIIGGAPLIPEHDPSVLFTTAGMHP
                     LVPFLLGESHPAGGRLANVQRCLRTNDIDEVGDGVHLTFFEMLGAWSLGDYWKREAQR
                     LSFGFLTERLGLDPARIWVTCFAGDTDAPRDDEAAEFWRELGIPDRRILFLPKADNWW
                     GPAGASGPCGPDSELFYDTNPTGDPDATPATDPARFWEVGNNVFMQYDLRADGRYHPL
                     GRNNVDLGIGLERVLALVEGVPSAYETELFAPIMDRIRTLAAHPAPFAVRVIADHTRA
                     AVNILAEGIVPGKVDQPYIARRLIRRAIRYGREIGIAGHFLADLAGVVIPTLSGPYPD
                     LAREQARIAAALDDEETRFQRTLAEGEKELAK"
     gene            complement(1708..>2326)
                     /locus_tag="VF276_05590"
     CDS             complement(1708..>2326)
                     /locus_tag="VF276_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxyneurosporene dehydrogenase"
                     /protein_id="HEX6606373.1"
                     /translation="DEHYFAWLPAVPQGDVDAAITLGGAPQHFTGIGYHDHNWGNISI
                     VRLIDHWYWARGAIGPYTLIASYITVAQRYGYRTVPVFMLARGGRIVADDGSKVRFAA
                     RAIQPDPATGKPVANLTRYEYRDAGHHYVLTFTRRNTILRMKLVEGIHGIQGALARLI
                     HFDGAFLRFTGDLHLVRYEGDQIVEAQQDEAIWELMYLAHAHAER"
BASE COUNT          349 a          765 c          805 g          407 t
ORIGIN      
        1 cttcgccagt tccttctcgc cctcagccag ggtgcgctgg aagcgcgtct cttcgtcgtc
       61 cagcgccgcc gcgatgcgcg cctgctctcg cgccaggtcg gggtacggcc cgctcagcgt
      121 ggggatgacc acgcccgcca gatccgccag gaagtggccc gcgatgccaa tttcgcgccc
      181 gtagcggatg gcgcggcgaa tcaggcggcg cgcgatgtag ggttggtcta ccttgcccgg
      241 cacgatcccc tcggccagga tgttgaccgc cgcgcgggtg tggtcggcga tgacccgcac
      301 cgcgaacggc gccgggtgcg ccgccagcgt gcggatgcgg tccatgatgg gcgcgaacag
      361 ttcggtctcg taggccgagg gcacgccctc gaccagcgcc agcacgcgct ccaggccgat
      421 gccgaggtcc acgttgttgc ggcccagcgg atggtagcgc ccgtcggcgc gcaggtcgta
      481 ctgcatgaag acgttgttgc ccacctccca gaagcgcgcc gggtcggtcg ccggggtcgc
      541 gtcggggtcg ccggtggggt tggtgtcgta gaacagttcg gagtccggcc cgcacggccc
      601 gctcgcgccc gccggtcccc accagttgtc tgccttgggc aggaacagga tgcgccggtc
      661 ggggatgccc agttcccgcc agaactccgc cgcctcgtcg tcgcgcggcg cgtcggtgtc
      721 gcccgcgaag cacgtcaccc agatgcgcgc cgggtccagg cccagccgct cggtcaggaa
      781 gccgaagctg agccgctgcg cctcgcgttt ccagtagtcg cccagcgacc acgcgccgag
      841 catctcgaag aaggtcaggt ggacgccgtc gcccacctcg tcgatgtcgt tggtgcgcag
      901 gcagcgctgg acattcgcca gccgcccgcc cgccgggtgg ctttcgccga gcaggaaggg
      961 caccagcggg tgcatgcccg ccgtcgtgaa cagcacgctc ggatcgtgtt cggggatcag
     1021 cggcgccccg ccgatgatcg cgtggccgcg cgcctggtaa tagtccagaa acgcccggcg
     1081 caaggtttgc gcatccatgg cttatgctcc tttcacattc gttataggct tgtcactctg
     1141 agcgcagcga agagtctcgt cttgtgcgcc aacgagacgg agattcttcg ctccgctcag
     1201 aatgacaccg gtgttgcctc ccgcgggaga caaataaaaa ccgcccatga gtccggagac
     1261 tcaagggcgg agacaaatcc gcggtaccac cttggttgga acagcacgct gctcccactc
     1321 tgccgccgct accgggccgc cgggggcggg ggtaagggcg cgtcctgcta acggcggact
     1381 tggccggcgc gccttcggcc ctcgcggggt tcggcggcaa ctcaggtgtg gcttcggcgc
     1441 ccgttagccc gtcgagctta cactctcctc gactcgctgg atgcctcacg gggcgcgtac
     1501 tcttcaccgt caacgtcggt ggtattcgat tgtgccctaa tcatacccgc tggcgggggc
     1561 cggtgtcaat cggccatccg gtgtatgcgc ttattaactt caccgcgaag gaacgaaggc
     1621 cgcgaagatt atttgtagta gttgtattct ttgcgcgctt cgttcctgcg cggtgcaaca
     1681 tcagtgtccg gcgccgacgg gcgacgctta gcgctcggcg tgcgcgtggg ccagatacat
     1741 caattcccag atcgcttcgt cctgctgcgc ctccacgatc tgatcgccct cgtagcgcac
     1801 caggtgcagg tcgccggtga agcgcaggaa ggcgccgtcg aagtggatca gtcgggccag
     1861 cgcgccctgg atgccgtgga tgccctcgac caacttcatg cgcaggatag tgttgcggcg
     1921 ggtgaaggtc agcacgtagt ggtgtccggc gtcccggtac tcgtagcgcg tgaggttggc
     1981 gaccggcttg ccggtggccg ggtcgggttg gatggcgcgg gcggcaaagc ggaccttgct
     2041 gccgtcgtcg gcgacgatgc gcccgccgcg ggccagcatg aaaacgggga ccgtgcggta
     2101 gccgtagcgc tgggcgacgg tgatatagga ggcaatcagc gtgtacggcc cgatcgcgcc
     2161 ccgcgcccag taccagtggt cgatcagccg cacgatggag atattgcccc agttgtggtc
     2221 gtggtagcca atgccggtga agtgttgggg cgcgccgccc aaggtgatcg ccgcgtccac
     2281 gtcgccctgc ggcacggcgg gcagccaggc aaagtagtgc tcgtcg
//
LOCUS       DASUYC010000340         3197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_410650,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000340 DASUYC010000000
VERSION     DASUYC010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410650"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VF276_05595"
     CDS             complement(<1..400)
                     /locus_tag="VF276_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX6606374.1"
                     /translation="MRIIVAGGAGFIGSHLTAALLADGHDVLILDNYLTGNPRNIAAL
                     RDNPRLRVIEHDISRPLPDLPQADQVYHLASPASPADFTRIPLEILATNTYGTQNLLE
                     WATQYNARILFTSTSEAYGDPLVHPQVETYF"
     gene            complement(433..>3197)
                     /locus_tag="VF276_05600"
     CDS             complement(433..>3197)
                     /locus_tag="VF276_05600"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX6606375.1"
                     /translation="VKAVVSRYKGKVRYFQIWNEPNLIGEWPPQPNAAAYTALLKAAY
                     EAAHAANPDAVILMAPLAQTHTGDQCNPNDILYLQAMYEAGARPYFDIANVMDYGLNY
                     SPADRRTDLDRINFSRPVLARDVMVRNGDGAKGIWASEYGWVSLPPDWDADPANKPGI
                     WGSVTPEQQANYLISGYDRARSEWPWMGAMFVWFLRDPAPLPHEPQPYFAILNPDWSQ
                     RPAYGAIARYSKRFPLAETGAGLPDNPAVTVAGPWTKAAPEGRPVYTATTASSTATLT
                     FEGNILDLVFAPGAKPAPVQVELDGGPPPGWPVDGTGRALATIPAAGGTATRVTPDAA
                     WPAPPGMSALRLRVAGDLKDVRHTLTIRPANVDAPVALLGFVVGRTSPQAGLFAAGYL
                     LLTGLALWLGVRSTFAVVALPRLVRQAVALPGLTGDGARAGVVVAAMAATLVAYYLLP
                     SPRWALLPLAVWFLLAVLRPDMAVVMTAAAIPFATAPRALLDWSFPVSETCLWLTLVA
                     WIIHRAWRELAHRRAAAAVAAAPADLTVTGYLNLYQELQGPVPWRERLGTALRRDLFG
                     VPAVALLLVGTFSLLTVANPEYLRDTLRMYRWVIVEPVLFYFLATEIRAGRRQALRVA
                     DGFIGGSALAAALALALGFLNVPGHTLDVQGVVRFQGLFTHPDNLGLFLGRALGFTAA
                     LALFLTAPGETLRRRAYMLATLVMIPALVLSYSRGAWLAFVVALGVSVWMVGGRAARW
                     FTGALVAGGAGLLALAALGRLPERIVHTGSGLIRLDLWQTALAMLRDHPIFGIGLDQF
                     LNQYQGPYADPAHQQERWLSHPHNLILDCWLSLGIIGVLVAGWILARVVLGALALIRR
                     TDARGAAMARAVLAGMTVVVVHGLVDNSYFLPDLALTFWLFCALLQMMWKQAADPAAG
                     RPTVRR"
BASE COUNT          504 a         1082 c         1201 g          410 t
ORIGIN      
        1 cgaagtaggt ctcgacctgc gggtggacca gcggatcgcc gtaggcttcg gaggtggagg
       61 tgaaaaggat gcgcgcgtta tactgcgtgg cccattcgag caggttctgc gtgccgtagg
      121 tgttggtcgc caggatctcc agcgggatgc gggtgaaatc ggcggggctg gccgggctgg
      181 cgaggtgata gacctggtcg gcctgcggca gatcgggcag gggccggctg atgtcgtgct
      241 cgatcacgcg cagccgggga ttatcacgca gcgcggcgat attgcgcggg ttgccggtga
      301 ggtagttgtc gagaatgaga acgtcatgcc cgtccgccag cagggcggcg gtgaggtgcg
      361 agccgataaa accggcccca ccggcgacaa tgatgcgcaa aggatcctcc tactggttgc
      421 cgggtgcggg ggctaacggc gaaccgtggg ccggccggcg gcgggatcgg ccgcctgctt
      481 ccacatcatc tgcaacaggg cacagaagag ccagaaagtg agcgccagat ccggcagaaa
      541 atatgagttg tccacgaggc catgcacgac cacaacggtc atcccggcca gcaccgcgcg
      601 ggccatcgcg gccccccgtg cgtcggtgcg acggatcagg gccagcgccc ccaggaccac
      661 gcgtgccagg atccaaccgg cgacaagcac acctattata cccaaactga gccagcaatc
      721 caagatcagg ttatgcgggt gcgacagcca gcgctcctgc tggtgcgccg ggtcggcgta
      781 ggggccttga tactggttga ggaactggtc gaggccgatg ccgaagatgg ggtggtcgcg
      841 gagcatggcg agcgccgtct gccacaggtc gaggcggatc aagccgctgc cggtgtgcac
      901 gatgcgctcc ggcaggcggc ccagcgcggc cagcgccagc agcccggccc cgcccgccac
      961 cagcgccccg gtgaaccagc gcgccgcccg cccgcccacc atccagacgc tcacgcccag
     1021 ggcgacaacg aaggccagcc aggccccgcg cgaatacgac agcaccagcg ccgggatcat
     1081 caccagcgtc gccagcatgt aggcgcggcg gcgcagcgtc tcgcccggcg cggtcaggaa
     1141 cagggccagc gcggcggtga agccgagcgc ccggcccagg aacaggccca ggttgtcggg
     1201 gtgggtgaac agcccctgga agcgcaccac cccctgcacg tcgagcgtgt ggccgggcac
     1261 gttgaggaag ccgagcgcca gcgcgagcgc cgcggccagc gccgacccgc cgatgaagcc
     1321 gtcggccacg cgcaacgcct ggcggcgccc ggcgcggatc tcggtcgcca ggaagtagaa
     1381 cagcaccggc tcgacgatca cccagcggta catccgtagc gtgtcccgca ggtactccgg
     1441 gttcgccacg gtgagcagcg agaaggtgcc caccagcaac agcgccaccg cgggcacgcc
     1501 gaacaggtcg cggcgcagcg ccgtccccag ccgctcgcgc cagggcacgg ggccctgtag
     1561 ctcctggtac aggttcaggt agccggtgac ggtgagatcg gcgggcgcgg cggcgacagc
     1621 ggcggcggcg cggcggtggg ccagctcgcg ccaggcgcga tggatgatcc aggcgaccag
     1681 cgtgagccag aggcaggtct cgctgaccgg gaagctccag tcgagcaggg cgcgcggcgc
     1741 cgtggcgaac gggatggcgg cggccgtcat caccacggcc atgtcgggcc gcagcacggc
     1801 cagcaggaac cagacggcca gcggcaggag cgcccagcgc ggagacggca gcaggtagta
     1861 ggcgaccagc gtggcggcca tcgcggccac gaccaccccg gcgcgcgccc cgtcgccggt
     1921 cagccccggc agggccacgg cctggcgcac cagccgcggc agggccacca cggcgaacgt
     1981 gctgcgcacc cccagccaga gcgccagccc cgtcagcagg agataccccg cggcgaacag
     2041 cccggcctgc ggcgacgtgc gcccgaccac gaagccgagc agcgccaccg gcgcgtccac
     2101 gttggccggg cggatagtca gggtgtggcg cacgtccttc aggtcgccgg ccacgcgcag
     2161 gcgcagggcg ctcatgccgg gcggcgcggg ccaggccgcg tcgggcgtca cgcgggtggc
     2221 cgtgcccccg gcggcgggga tggtggcgag ggcgcggccc gtgccgtcca ccggccagcc
     2281 gggcggcggc ccgccgtcga gttcgacctg caccggcgcg ggtttcgcgc ccggcgcgaa
     2341 caccaggtcc aggatgttgc cctcgaaggt cagggtcgcg gtgctgctgg ccgtggtcgc
     2401 cgtgtagacc gggcggccct ccggcgccgc cttggtccac ggcccggcca ccgtcacggc
     2461 tgggttgtcg ggcaggcccg cgcccgtctc ggccagcggg aagcgcttgc tgtagcgggc
     2521 gatagcgccg taggccgggc gctggctcca gtcggggttg aggatggcga aatagggctg
     2581 gggttcgtgc ggcaggggcg cggggtcgcg caggaaccag acgaacatcg cgcccatcca
     2641 gggccactcg ctgcgtgccc ggtcgtagcc gctgatcagg tagttcgcct gctgctccgg
     2701 cgtcacgctg ccccagatgc ccggcttatt cgccgggtcg gcgtcccagt cgggcggcag
     2761 gctgacccag ccgtactcgc tggcccagat gcccttggcg ccgtcgccgt tgcgcaccat
     2821 cacgtcgcgc gccagcaccg ggcgcgagaa gttgatgcgg tcgagatccg tgcgccggtc
     2881 ggcgggcgag tagtttagcc cgtagtccat gacgttggcg atgtcgaagt agggcctggc
     2941 gcccgcctcg tacatggcct gcaagtagag gatgtcgttg gggttgcact ggtcgccggt
     3001 gtgcgtctgg gccagcggcg ccatcaggat caccgcgtcg gggttggcgg cgtgggcggc
     3061 ttcgtaggcc gctttcagca gtgcggtata ggcggcggca ttgggctgcg gcggccactc
     3121 gccgatcagg ttcggctcgt tccagatctg gaagtagcgc accttgccct tgtagcgcga
     3181 cacgacggcc ttgacga
//
LOCUS       DASUYC010000341         2837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1161729, whole genome shotgun sequence.
ACCESSION   DASUYC010000341 DASUYC010000000
VERSION     DASUYC010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161729"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="VF276_05605"
     CDS             complement(<1..674)
                     /locus_tag="VF276_05605"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606376.1"
                     /translation="MAKITELLLVPHTHHDIGYTHVPDVCMRMHEQAIYEAMGLCEAA
                     LADDSPAAFRWTVEISRPLLAFLRGAVPADVARLQALVARGRMAVTAGYLHMTQLIGH
                     EEYVRFFLPVREIRERYGLPVSIVQHGDINGLSWGVVPLMREAGLDCLVMALNPDHGR
                     APFEQPSVFWWAGPDGARVLVCLNPHYSTTNNPWELTAGRIEAAIAPLAALVARTEAR
                     SDYPFD"
     gene            1064..>2837
                     /locus_tag="VF276_05610"
     CDS             1064..>2837
                     /locus_tag="VF276_05610"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX6606377.1"
                     /translation="MKRSTLVLFYAAIGLSFVLVGARLVQMQVVDGARYQLQADSNRI
                     REVSVDAPRGVIYDRNLRQLVSNQPAFSVAATEADMPEDPAEQQGVFDRLATLLNTKP
                     VVTGEPDKLFADPVLATKVVGQLATLLKVPVSELNKTLADARRISPEAPNLLRSDLDA
                     ATAAAITAHKDDWPGIEVMNELQYNYITHHDNPIHPVTVERNIPFETMQRVEEAHLDL
                     PGISVVSEPVRQYALGSFMSHILGYVGPIPPDQYAASLPPEGSLDPPPYDKDDKVGLL
                     GVEASMENALRGAKGMSQIEVNANQREVREIRSVPPVPGQNVVLTIDSALQVTVTHLL
                     QEGIDAAHTGSGTRAGVAIVENVQTGEILSMVSLPSYDDNLFAAGISQADFDRLNNDP
                     NLPLFHRAIGGAYPPGSTLKMITASAGLQTGVITPLTQIFCPGLISVPLTYNEKQRTP
                     YYDWKRAGHGTLTVREALQQSSDVFFYEVAGPGQLDALGHLTHFYVPGDPNPHPFSGL
                     GIDKLGNYMELFGMGERTGIDLPGEIPGVVPSPAYKEGLNPADKWSLGDTLFTAIGQG
                     FDLSTPLQLTNVTAAVANGGTLYRP"
BASE COUNT          506 a          989 c          899 g          443 t
ORIGIN      
        1 aagtcgaagg ggtagtcgct tcgcgcctcg gtgcgcgcca ccagtgccgc cagcggcgcg
       61 atggccgctt cgatgcgccc ggcggtcagt tcccaggggt tgttggtggt gctgtagtgc
      121 gggttcaagc agaccagcac gcgcgccccg tcgggccccg cccaccagaa gaccgacggc
      181 tgctcgaacg gcgcccgccc gtggtcgggg ttgagggcca tgaccaggca atccagcccg
      241 gcctcgcgca tcagcggcac cacgccccag ctcagcccgt tgatgtcgcc gtgctgcaca
      301 atcgacaccg gcagcccgta gcgttcgcgg atctcgcgca cgggcaggaa gaagcgcacg
      361 tactcctcgt ggccgatcag ttgcgtcatg tgcaggtagc ccgccgtgac cgccatgcgc
      421 ccgcgcgcca ccagcgcctg caagcgggcc acatcggcgg gcaccgcccc gcgcaaaaac
      481 gcgagcagcg ggcggctgat ctccacggtc cagcggaagg cggcgggcga atcgtcggcc
      541 agcgccgcct cgcacagccc catcgcctcg tagatggcct gctcgtgcat gcgcatgcac
      601 acgtcgggca catgcgtgta accaatgtca tggtgcgtat gcggcaccag caacaactcg
      661 gtgattttcg ccatggatcc gccccccggc gcggcgatgc ccgcagccgt tatatgcagc
      721 agtgctaaaa tcagcccaaa cccagtatag taacgcgttc cggcaccgtc aaggcacgtt
      781 gctgccgtcc cattatattc accgcgaagg cgcgaagaac gcgaaaactg tttgtacaag
      841 tcatattctt cgcgtccttc gcgtcttcgc ggtgaattta cggccggata gcccgcgaat
      901 cgcttgcggt aacgggccgg taaggtttat actagacccc gattgcctgt ttgcgtgacc
      961 ccccttgcca cgcctctgct tgccgcggcc cttcggcagg aagtgaatcg tgcacccggc
     1021 gccggcctgg gacggcggcg cgcggggtga gaattgttgc ctcatgaaac gttcaacgct
     1081 ggttttattt tatgccgcga tcggcctgtc gtttgtcctg gtcggcgccc ggctagtgca
     1141 gatgcaggtg gtggacggcg cgcgctacca gttgcaggcc gacagcaacc gcatccgcga
     1201 ggtgagcgtg gacgcgccgc gcggcgtcat ctacgaccgc aacctgcgcc aactggtcag
     1261 caaccagccg gccttctccg tggccgccac cgaggccgat atgcccgagg accccgccga
     1321 gcagcagggg gtgttcgacc ggctggcgac cctgctcaac accaaaccgg tggtcacggg
     1381 cgagcccgac aagctcttcg ccgatcccgt gcttgcgacg aaggtcgtgg gccaactggc
     1441 gacgctgctc aaggtgccgg tgagcgaact caacaagacg ctggccgatg cgcgccgcat
     1501 ctcgcccgaa gcgcccaacc tgctgcgcag cgacctcgac gcggccacgg cggcggctat
     1561 caccgcgcac aaggacgact ggccgggcat cgaggtgatg aacgagttgc agtacaacta
     1621 catcactcac cacgacaacc cgatccaccc ggtcacggtc gagcgcaaca tccccttcga
     1681 gaccatgcag cgggtggaag aggcgcatct cgacctgccc ggcatcagtg tggtttccga
     1741 gccggtgcgc cagtacgcgc ttggctcgtt catgagccac atcctgggct acgtcggccc
     1801 catcccgccc gaccagtacg ccgcctcgct gccgccggaa ggcagcctcg acccgccgcc
     1861 ctatgacaag gacgacaagg tgggcctgct gggcgtcgag gcgagcatgg agaacgccct
     1921 gcgcggcgcg aagggcatga gccagatcga ggtcaacgcc aaccagcgcg aagtgcgcga
     1981 gatccgcagc gtgccgccgg tgcccggcca gaacgtggtg ctgacaatcg acagcgcgct
     2041 gcaagtgacc gtgacccacc tgttgcagga aggcatcgac gcggcgcaca ccgggagcgg
     2101 cacgcgggcc ggggtggcga ttgtcgagaa cgtgcagacc ggcgagatcc tctccatggt
     2161 cagcctgccg tcgtacgacg acaacctgtt cgcggcgggc atctcgcagg ccgatttcga
     2221 ccggctgaac aacgacccga acctgccgct gttccaccgg gccatcggcg gcgcctaccc
     2281 gcccggctcg acgctcaaga tgatcaccgc ctcggcgggc ttgcagacgg gcgtcatcac
     2341 gccgctgacg cagatcttct gccccggcct catctcggtg ccgctgacct ataatgagaa
     2401 gcagcgcacg ccctactacg actggaagcg cgcgggccac ggcacgctga ccgtgcgcga
     2461 agccttgcag caatcgtccg atgtgttctt ctacgaggtg gccgggccgg gccagttgga
     2521 cgccctgggc cacctgaccc acttctacgt gcccggcgac cccaacccgc atcccttcag
     2581 cggcctgggc atcgacaaac tgggcaacta catggaactg ttcggcatgg gcgaacgtac
     2641 cggcatcgat ctgccgggcg agatccccgg cgtggtgccc agcccggctt ataaagaggg
     2701 cctgaacccc gccgacaagt ggtcgctggg cgatacgctg ttcacagcca tcggccaggg
     2761 cttcgacctc tcgaccccgc tgcaactgac caacgtgacc gcggcggtgg ccaacggcgg
     2821 cacgctctat cggccgc
//
LOCUS       DASUYC010000342         5616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1619038, whole genome shotgun sequence.
ACCESSION   DASUYC010000342 DASUYC010000000
VERSION     DASUYC010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5616
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1619038"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1997)
                     /locus_tag="VF276_05615"
     CDS             complement(<1..1997)
                     /locus_tag="VF276_05615"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX6606378.1"
                     /translation="MPRLTLALLGGFQAAIDGTPVAGFDTDKTRALLAYLAVEAGRPH
                     ERETLAGLLWPELPDDAARRNLRNSLFKLRQALGEEDGAPGFLRITLKTVQIDPDAEV
                     VVDAGAFSGLVAACMAHRHRHVERCATCHARLAQAAALYQGAFLTGFHLGDCAAFEEW
                     LVLTRERLHRAAMQTLGRLAAYHEGRGEGAPALEYTFRQLALEPWREEAHRQAMRLLA
                     RSDQRSAALAQYAACRKALAEELNAEPGAETTALYDRLRAGALPDDPAALPDAPNALH
                     RMPQPLTPFIGREAELRLIIERLDSPDYRLITVIGPGGVGKTRLAWQAAAEQVGAFAG
                     GVYWVPLADLTEPDLIPATIGQAVGLRFETADNPAAQLVNYLRDKEMLLVLDNFEQLQ
                     SRAATDLLPAILRGAPRVSLLVTSRERLGLQAEVVLDLAGLPLPDAVQAAPAARQGSR
                     GPVAVAPALLEPATLLASGAVQLFVERAQQAQPAFALTAETAPGVVEICRLVAGLPLG
                     IVLAAAWVRHFPPARIAAALRANLDFLASRAADLAPQHRSLRAVFDHSWHLLPATEQR
                     GFRGLAVFSGGWDDEAAARVAGAPLGALLSLVDKSLVRQDSAGRFHVHEVLRQYAAER
                     LADVPGEQAQVRQDHAAYFLELAELAATGLSGETQGQALARL"
     gene            2377..2622
                     /locus_tag="VF276_05620"
     CDS             2377..2622
                     /locus_tag="VF276_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606379.1"
                     /translation="MGSQDHARPEYLSYLLRLWHTHSGGETVWRAALEDPLTREVVRF
                     DNLAGLFAFLSGRIGEGGTEGSSAPAASHDNEGSIRA"
     gene            2619..5585
                     /locus_tag="VF276_05625"
     CDS             2619..5585
                     /locus_tag="VF276_05625"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEX6606380.1"
                     /translation="MKGQAHPHTRGRGWWLGLVALAVLWLLGGAAAGGRPVAGAPASR
                     PPPPGCNICNLSFSSVTSICRTDNSLGWTAVVANGNTGCTVTDVYTASLQVHMPGQAP
                     GQYQTIATQSGVRSFAPGATTLSGAFCATLPAGANSVRVSVDLDRQGATCSLSGRSVA
                     QAPCPACFTPTPTPTNTPTNTPTNTPTNTPTNTPTNTPTNTPTDTPTNTPTPTNTPTN
                     TPTNTNTPTNTPTNTSTPTNTATNTPTNTPSNTPTNTATVTPTNTATNTATNTPTNTP
                     TPTPVATNTPTATPTPCVAWGRGLAAPTPASATGVGSDGTVIYAAGGTTGLNSVTGRF
                     ARYNPATNTWTNLSPMPAPAYAPSVAVLNGKVYVIGGADGGNNGLTANRIYTIASDTW
                     SSGAPLPAGRVYMASGVFNNRIYVTGGMSNGQTGSALGNTWEYDPVANVWNPRAAMPG
                     GRTYAGFGVINDHLYVAAGQNSYTGLQDYNMATDTWTSRASMPGPGVYLPGSASIGGR
                     LWIFGGIIGTGPDFGTVTSATWLYDPVSNSWAAGPNLPSGHFDIGGAGAGTYAVGIGG
                     TLTDIADTTCGGLITPTYTPTPTSTPGSPTATPTNTFTVTPTFTPSNTPTLTPTRTVT
                     RTPTATPTPCLPFYRTDYAAGSHPSGIVTGDLNGDGRPDLVIANSNGPTTVSVLPAQA
                     GGGFAAPTAYTVPYTPQELALGDLNADGRLDLAVTNYDISTPPGTNFVSVFYGNGAGG
                     FSTTRTDLTTGSRPEGVVIADINRDGRTDIATADYADGNVSILYGTAGGFVRLDYPAN
                     ISPYGLIAGDFNGDGRLDLVVTNFNQDFNGNTNVSVLLNNQNGVAAPVSYRIGGTFPT
                     RLVAGDFNGDGHPDLAVPNFQQNNVSVLLNNGSGGFGGPANYAVGFNPTGVTVGDFDG
                     DGRLDLATADRGTNDASVLRGDGQGGFAAAQAFTTGPGPSGLVAADLNGDGRTDLAVA
                     DDNDSVSALLNGGCWP"
BASE COUNT          912 a         2095 c         1869 g          740 t
ORIGIN      
        1 agccgcgcca gcgcctggcc ctgcgtttcg ccgctcaggc cggtcgcggc cagttcggcc
       61 agttcgagaa aataggccgc gtgatcctgc cgcacctggg cctgctcgcc gggcacgtcg
      121 gccagccttt ccgccgcata ctggcgcaac acttcgtgga cgtggaagcg cccggcgctg
      181 tcttgccgca ccagcgattt atcgaccagc gacaacagcg cgccgagcgg cgccccggcc
      241 acccgcgcgg cggcttcgtc gtcccacccg ccgctgaaca ccgccagccc gcgaaagccc
      301 cgctgctcgg tggccggcag caggtgccag gagtggtcga acacggcgcg caggctgcga
      361 tgctggggcg cgaggtcggc ggcccggctg gcgaggaagt cgagattggc gcgcagggcc
      421 gcggcgatgc gcgccggggg gaagtggcgc acccaggccg cggccagcac gatgcccagc
      481 ggcagcccgg ccaccagccg gcagatctcc accacccccg gcgccgtctc cgccgtcagc
      541 gcgaacgccg gctgcgcctg ctgcgcccgc tcgacgaata actgcaccgc gcccgacgcc
      601 agcagcgtgg ccggctccag cagggccggc gcgaccgcca ccggcccccg gctgccctgg
      661 cgcgcggcgg gggccgcctg cacggcgtcg ggcaggggca ggcccgccag gtccagcacg
      721 acttcggctt gcaggcccag ccgctcgcgc gacgtgacca gcaggctgac ccgcggggcg
      781 ccgcgcaaga tcgccggcag caggtcggtg gcggcgcggc tctgcaactg ctcgaaattg
      841 tcgagcacca gcagcatttc cttatcgcgc aggtagttaa ccagttgcgc cgccgggttg
      901 tccgctgtct cgaaccgcag gcccaccgcc tgcccgatgg tcgcggggat caggtcgggc
      961 tcggtcaggt cggccagcgg cacccagtag acgccgccgg cgaacgcgcc cacctgctcg
     1021 gcggcggcct gccaggccag ccgggtcttg cccacgccgc cggggccgat cactgtgatc
     1081 agccggtaat cggggctgtc gagccgctcg atgatcagcc gcagctcggc ttcccgcccg
     1141 atgaacggcg tcagcggttg gggcatgcgg tgtaaggcgt tgggcgcgtc gggcagggcg
     1201 gcggggtcgt cgggtagggc gcccgcgcgc agccggtcgt agagcgcggt cgtctcggcg
     1261 cccggctcgg cgttcagttc ctcggccagg gctttgcggc aggccgcgta ctgggccagg
     1321 gcggcgctgc gctggtcgct gcgcgccagc aggcgcatgg cctggcgatg ggcctcctcg
     1381 cgccacggct ccagcgccag ttggcggaag gtgtactcca gggcgggcgc gccctcgccg
     1441 cggccctcgt ggtaggcggc cagccggccc agcgtctgca tggcggcccg gtgcagccgc
     1501 tcgcgcgtga gcaccagcca ttcctcgaag gccgcgcaat cgcccaggtg gaagccggtc
     1561 aggaacgcgc cctggtacag cgccgccgcc tgcgccaggc gcgcgtggca ggtggcgcag
     1621 cgctcgacgt ggcggtggcg gtgcgccatg cacgcggcga ccagcccgct gaacgccccg
     1681 gcatcgacca cgacctcggc gtccgggtcg atctgcaccg tcttgagcgt gatccgcagg
     1741 aagccgggcg cgccgtcctc ctcgcccagg gcctggcgca gcttgaacag cgaattgcgc
     1801 aggttgcggc gcgcggcgtc gtccggcagt tcgggccaga gcaggcccgc cagcgtttcc
     1861 cgctcatgcg ggcgcccggc ctccacggcc aggtaagcca gcagggcgcg ggtcttatcg
     1921 gtatcgaagc cggccaccgg cgtaccgtcg atggcggcct ggaagccacc cagcagggca
     1981 agggttaagc gaggcatgtg ggagtggggg cgaacatggg acgacgcctg cgacgtaagg
     2041 gcagatacgc ccgccgcggc acgcggaggc ggcgggcaag aaaggcgtgg acactttcct
     2101 tgctactata cgctagtccg gcgcgcttgg caacgatccg gacccgaatt acaccgcgaa
     2161 gaaacgaagg acgcgaagaa cacacctgat agaaaccgtc ttcgcgttct tcgtttcttc
     2221 gcggtgtaat tccgagcgta tgcgcgcccg gcccccgatt caacgctttt tcaacacccc
     2281 ctcttgtatg atcccggcaa gcgtacgagc cccgccggcc ggcagccggg ggcacgcggg
     2341 gagttcgcac cgctaacacc ggggggagcc atcactatgg gcagccagga tcacgcgcgt
     2401 ccggaatacc tgtcctatct gctgcgcctc tggcacaccc acagcggggg cgagacggtg
     2461 tggcgggccg cgctggaaga cccgctcacg cgggaggtcg tacgcttcga taatctggcc
     2521 ggcctgttcg ccttcctgtc gggccggatc ggggaggggg gcacggaagg cagcagcgcg
     2581 ccggccgcat cgcacgacaa cgaagggagc atacgcgcat gaaagggcag gcgcatcccc
     2641 acaccagggg gcgaggatgg tggctggggc tggtggccct ggcggttcta tggttgttgg
     2701 gcggcgcggc ggcgggcggc aggcccgtgg ccggggcgcc cgcctcgcgc ccgccgccgc
     2761 cgggctgcaa catctgtaac ttgagcttca gcagtgtcac cagcatctgc cgcacggaca
     2821 acagcctggg ctggacggcg gtcgtcgcca acggcaacac cggctgcacc gtgaccgatg
     2881 tctacaccgc cagtttgcag gtccatatgc cggggcaggc gcccggtcag taccagacga
     2941 tcgccaccca gagcggcgtg cgcagctttg cgccgggcgc cacgacgttg agcggggcct
     3001 tctgcgccac gctcccggcc ggcgcgaaca gcgtgcgcgt gtcggtcgat ctcgatcggc
     3061 agggcgccac ctgcagcctg agcggtaggt ccgtggccca ggcgccctgc ccggcctgct
     3121 tcacgcccac gcccaccccg acgaacacac cgaccaacac gccgaccaat accccgacga
     3181 acacgccgac gaacacgccg acgaacacgc cgacgaatac gccgacggat acgccgacca
     3241 acaccccgac gccaacgaat acgccgacca acacgccgac caataccaat acgccgacca
     3301 acaccccgac caataccagt acgccgacca acaccgcgac gaacaccccg accaatacgc
     3361 ccagcaatac gccgaccaat acggccaccg ttaccccgac gaatacggcg accaatacgg
     3421 cgacgaacac gccgaccaac acccccacgc cgacgcccgt cgccaccaac accccgacgg
     3481 ccacgccgac gccgtgcgtg gcgtggggcc gcggcctagc ggccccgacc ccggccagcg
     3541 ccaccggcgt gggcagcgac ggcacggtaa tctacgccgc cggcggcact accggcctca
     3601 atagcgtcac cggccgcttc gcccgctata acccggccac caatacctgg acgaatctct
     3661 cgcccatgcc ggccccggct tacgcgccca gcgtcgccgt gctcaacggc aaggtctatg
     3721 tcatcggcgg cgccgatggc gggaacaacg gcctgaccgc caaccgcatc tacaccatcg
     3781 cctcggacac ctggagcagc ggggcccccc tcccggccgg ccgcgtttat atggcctccg
     3841 gcgtcttcaa taaccggatc tacgtgaccg gcgggatgag caatgggcag accgggagcg
     3901 cgctcggcaa cacctgggaa tacgacccgg tcgccaacgt ctggaacccc agggccgcga
     3961 tgccgggcgg gcgcacctat gccgggttcg gcgtgatcaa cgaccacctc tatgtcgccg
     4021 ccgggcagaa cagctacacc ggcctgcagg actataatat ggccaccgac acctggacca
     4081 gccgcgcctc gatgcctggc cccggcgtct atctccccgg cagcgcctcc atcggcggac
     4141 ggctctggat ctttggcggc atcatcggca ccggccccga cttcgggacc gtaacgagcg
     4201 ccacctggct ctatgacccg gtgtcgaaca gttgggcggc cgggccgaac ctgccctcgg
     4261 ggcattttga catcggcggg gcgggcgccg ggacctacgc ggtggggatc ggcgggacct
     4321 tgaccgatat cgccgatacg acctgcggcg gcctgatcac cccgacctac acgcccacgc
     4381 cgaccagcac gccgggctcg cccacggcca cgcccaccaa cacctttacg gtgactccca
     4441 ccttcacccc gtccaacacg ccgacgctga cgccgacgcg caccgtcacc cgcacgccga
     4501 cggccacgcc caccccgtgc ctgccgttct accgcaccga ttacgcggcg ggcagccacc
     4561 cgtcgggcat cgtgacgggc gacctgaacg gcgacggccg gcccgacctc gtcattgcca
     4621 actccaacgg ccccacgacc gtgtcggtgc tgccggcgca ggcgggcggg ggcttcgccg
     4681 cccccaccgc ttacaccgtg ccgtataccc cgcaagagct ggccctgggc gacctcaacg
     4741 ccgacggccg cctcgatctc gccgtgacca actatgatat cagtacccct cccggtacca
     4801 acttcgtgtc ggtgttctat ggtaatggcg cgggcggctt cagcaccacc cgcaccgatc
     4861 tcaccaccgg ctcgcggccg gagggcgtgg tcatcgccga catcaaccgc gatgggcgca
     4921 ccgacattgc caccgccgac tacgcggatg gtaacgtgtc gatcctctat gggacagccg
     4981 gcggcttcgt ccgcctcgat taccccgcca atattagccc ctatggcctg atcgcgggcg
     5041 acttcaatgg ggatgggcgg ctcgacctcg tcgtcaccaa cttcaaccag gacttcaacg
     5101 ggaacacgaa cgtgtcggtg ctgctgaata accagaacgg cgtcgccgcc ccggtcagct
     5161 accgcatcgg cggcaccttc ccgacccggt tggtggcggg cgacttcaac ggggatgggc
     5221 atcccgacct cgccgtgccg aacttccagc agaacaacgt gtcggtgctg ctgaacaacg
     5281 gcagcggcgg cttcggcggg ccggccaatt acgccgtcgg cttcaacccc accggcgtga
     5341 ccgtgggcga cttcgacggg gacggccggc tcgacctcgc caccgcggat cgcggcacca
     5401 acgacgcgtc ggtcctgcgg ggggacggcc agggtggctt cgccgccgcc caggccttca
     5461 ccaccggccc cggtccgtcg gggctggtcg ccgccgacct gaacggcgat gggcgcaccg
     5521 acctcgccgt cgccgacgac aacgacagcg tatcggccct gctgaacggc ggctgctggc
     5581 cgtaacgcaa gcaccacggg gcgggcgggc gtgggg
//
LOCUS       DASUYC010000343         2726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_778749,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000343 DASUYC010000000
VERSION     DASUYC010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778749"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..1171
                     /locus_tag="VF276_05630"
     CDS             137..1171
                     /locus_tag="VF276_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX6606381.1"
                     /translation="MRQVDMRKLPAAAQEERRRQVIGLRQSGMTYQAIAELVGLSPTG
                     VLNICHRFAAEGVKGLVSKPRGRKPGEQCLLNPAQEAEIQALIRRHTPDELGLPFALW
                     NRAAVGMLIARHCGVELAVRTVGKYLARWGFTAQKPIRRAYEQDPVAVRRWLRRDYPE
                     IVKRAKQARGLVVWGDETGLRADDVRGRSYAPRGRTPVVRVRQTRIKLSLITAVTNQG
                     KLRWMVVDGAVNGPTFIRFLERLIREARGKVFLIVDRLKAHRARLVRDWLEVHRSEIE
                     VHYLPSYSPELNPDEGVNADLKQALPRQEPARSKEQLRRAADRHMRSLSRRPQRIRAI
                     FRHPQFRYAA"
     gene            1331..1612
                     /locus_tag="VF276_05635"
     CDS             1331..1612
                     /locus_tag="VF276_05635"
                     /inference="COORDINATES: protein motif:HMM:NF024776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6606382.1"
                     /translation="MRQLATSAAHQRTRERFLALYEVTQASCATQVAARTQRHPQTVM
                     EWLHLYNAGGSEALIYRRTGGRRPLFAPRSRPGSARLSTPPSRPRRRRR"
     gene            1549..2391
                     /locus_tag="VF276_05640"
     CDS             1549..2391
                     /locus_tag="VF276_05640"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX6606383.1"
                     /translation="MEARLSEVVHAAQQTAAAPPLAGADPAPRWTLRRLVGWVEATLG
                     RRCCRETIRRALHRLKLSWKKARKLLGRADPQRREAFITQVQNLLAGAQHDRHLLVYL
                     DEAHIHQDADLGYGWAARGQRFWVASHSPRLADKLSFYGLYLYNEGEVRLWPYARANG
                     ANTIDVLHRLRAEWPDQPLMLVWDGAPYHRAQAVREAAAALQIELVPLPGYSPDFMPV
                     EALWRWLREDVTYHHCHRTAEDLSRRVAAFEAQLNRDPYAVADRLWVKDRLDPEEEKL
                     RFSM"
     gene            complement(2399..>2726)
                     /locus_tag="VF276_05645"
     CDS             complement(2399..>2726)
                     /locus_tag="VF276_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX6606384.1"
                     /translation="TRLRKGAPWLKTALVQCAWAATRKKGSYLQAQFHRLRQRRGPKK
                     AICAVAASILTAAWHMLRNGTFYQDLGPDHFSRRAQGGRTARLVKQLTALGYTVQPPA
                     QQLVSC"
BASE COUNT          437 a          903 c          951 g          435 t
ORIGIN      
        1 agactcagat gacgcagcct gcacctaccg tcccgtcgtc catcgaccgc ggcccgccaa
       61 cggaagccct agccgggcta ttgatccggc cctcattgtc ttgagctgag gctgggatgc
      121 tccaaattgg gcgggcatga ggcaggtgga catgcgcaag ctgccggccg cggcccaaga
      181 ggagcgccgg cggcaggtca tcgggcttcg ccagagcggg atgacctatc aggcaattgc
      241 cgagttggtt gggctgagcc cgaccggggt gctcaatatc tgccaccgct tcgcggccga
      301 gggcgtgaaa gggctggtca gcaagccacg cgggcgcaag ccaggcgagc agtgcctgct
      361 gaacccagcg caggaggcgg agatccaggc gctgatccgc cgtcatactc cggacgagtt
      421 gggcctgccc tttgccctct ggaaccgggc ggcggtgggg atgctgatcg cccgccactg
      481 tggggtcgag ttggcggtgc gcacggtggg caagtatctg gcgcgctggg gcttcaccgc
      541 gcagaagccg atccggcgcg cctacgagca ggacccggtg gcggtacggc gctggctgcg
      601 gcgggactac ccagagatcg tgaagcgcgc caagcaggcc cgcggcctcg tggtctgggg
      661 tgatgaaacc ggcctgcgcg cggatgacgt gcgcggccgc tcctacgctc ctcggggccg
      721 gaccccggtg gtgcgggtcc gccagacccg gatcaagctg agcctgatca cggcggtgac
      781 caaccagggc aagctgcgat ggatggtcgt cgatggcgcg gtgaacggcc cgaccttcat
      841 tcgcttcctt gagcgcctga tccgagaggc ccggggcaag gtcttcctca tcgtggaccg
      901 gctcaaggcc caccgcgccc gcctggtgcg ggactggttg gaggtgcacc gttcggagat
      961 cgaggtgcac tacctgccgt cctacagccc cgagctcaat ccggatgaag gcgtgaacgc
     1021 ggacttgaag caggcgctcc cgcgccagga gccggcgcgc agcaaggaac agctgaggcg
     1081 agctgccgac cggcatatgc gcagcctgtc cagacggccg cagcggatcc gcgccatctt
     1141 ccggcatccg cagttccgct acgccgcatg atcaagataa tgagggccgg atcaataaac
     1201 cgcgtccact ttgggaggca tggtttttga gggccgggct ccctatctat ggttagagac
     1261 aaatcggaga gtatctaccc ctagcatgtt gcgcgttgac tatgaccggt ggggccaaac
     1321 accggaagac ttgcgtcagt tggccaccag cgcggcccat cagcgcaccc gcgagcgctt
     1381 cctggcgctt tacgaggtca cccaggcgag ctgcgcgacg caggtggcgg cccgcaccca
     1441 gcgccacccg cagacggtga tggagtggct gcacctctac aatgcgggcg gctccgaggc
     1501 gctgatctac cggcgcacgg gtggtcgccg cccccttttt gccccgcgat cgaggccagg
     1561 ctcagcgagg ttgtccacgc cgcccagcag accgcggcgg cgccgccgct agccggggct
     1621 gacccggcgc cacgctggac cttgcggcgt ctggtcggct gggtggaggc gacgcttggc
     1681 cgccgctgct gccgcgagac gatccgccgc gccctgcatc gcctgaagct gtcgtggaag
     1741 aaggcccgca agctgctcgg ccgggccgac ccgcagcggc gagaggcctt catcacgcag
     1801 gtgcagaatc tgctggccgg tgcccagcac gaccgccacc tactggtcta cctcgacgag
     1861 gcacacattc atcaggatgc cgatcttggt tatggctggg cggcgcgcgg ccagcgcttc
     1921 tgggtggcct cgcattcacc gcgcttggcc gacaagctct ccttctatgg cctctacctc
     1981 tacaacgagg gtgaggtgcg gctctggccc tacgctcgcg ccaacggcgc gaacaccatc
     2041 gacgtcctgc accggctgcg ggccgaatgg cccgaccagc cgctcatgct ggtgtgggac
     2101 ggcgcgccct atcaccgcgc ccaggcggta cgcgaggccg ccgccgcatt gcagatcgaa
     2161 ctcgtgccct tgccgggcta cagccccgac ttcatgccgg tcgaggcgct ctggcgctgg
     2221 ctgcgcgagg acgtcaccta ccaccactgc catcgcaccg ccgaggatct gagccgccgc
     2281 gtcgccgcat tcgaggcgca gctcaatcgg gacccctacg ccgtcgccga ccgcctctgg
     2341 gttaaggatc ggctcgaccc cgaggaggag aaactacgtt tctcgatgta gacgcggttt
     2401 agcaggaaac cagttgctgt gctggcggct ggacggtgta gccaagggcg gtgagttgct
     2461 tgacgaggcg ggcggttctg ccgccctggg cccggcggct gaagtggtcg ggccccaggt
     2521 cctggtagaa ggtgccattg cgtagcatgt gccaggcggc ggtcaggatg gaggcggcga
     2581 ccgcacagat cgccttcttg ggcccacgcc gctggcgcag gcgatggaac tgcgcctgga
     2641 gatagctgcc cttcttgcgg gtcgcggccc aggcgcattg gacgagggcg gtcttgagcc
     2701 agggcgcgcc cttgcgcagg cgggtc
//
LOCUS       DASUYC010000344         3818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_939913,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000344 DASUYC010000000
VERSION     DASUYC010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939913"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..2634
                     /locus_tag="VF276_05650"
     CDS             136..2634
                     /locus_tag="VF276_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6606385.1"
                     /translation="MPSNGFTERAQEALRNAQEIVQQKRHSQLDVEHILLALLKPRDG
                     LISKIIEKLNGDPRALARRLDDQLNTSPRLYSSYSGLAQIHISMRAQRVVSAAAEEAA
                     KLNDEYIGVEHLFLAITAERGGTAARLLSEINIDQASVYTALQEIRGNARITDPGADG
                     RYQALERYSHDLTRMAREGQLDPVVGREAEIRRVMQVLCRRTKNNPVLIGEPGVGKTA
                     IVEGLAQQIATGDVPALLRNKRVLALDMGSLVAGSRFRGEFEERLKAVMDEVKQASKE
                     IILFIDELHTVIGAGAAEGALDAANMLKPALARGELQCVGATTLDEYRKHIEKDAALE
                     RRFATVFVEEPTVEATLAILRGLRPRYEEHHGLKITDSAVDAAVELSNRYIQDRFLPD
                     KAIDLMDEAASKVRLEVFSLPADVRELEALMHDLEEQMEEAGQRQDYAEAARLKAELL
                     LHEETYSQKRDAFFAEHNIDEIVDEEDVADVVARWTGIPVKRMLADEMQKLVDMEEHL
                     HKRVIGQDAAIAAVSDAIRRARAGLKDPRRPMGSFIFVGPTGVGKTELAKALAEFMFD
                     DENAIVRVDMSEYGERHTVARLIGSPPGYVGYDEGGQLTEAVRRRPYQVVLFDEIEKA
                     HPEVFNALLQVLDDGRLTDGHGRTVDFRNTLIILTSNVGTQFMPQYGGLGFRTPGAAR
                     DTRNEEIETRHRVERAMKESFRPEVLNRIDDTILFSALSLDELRQIVDIQVRELTERM
                     GVQQLRLELTDEARTWLAERGYDRTFGARPLKRLIQRELETPISKLLLRGDLKAGQGI
                     LATVAEDGSRLMPEVVGEPAVALPVTQDADAQVG"
     gene            complement(2794..3360)
                     /locus_tag="VF276_05655"
     CDS             complement(2794..3360)
                     /locus_tag="VF276_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606386.1"
                     /translation="MNSYRLALFVMAAVVLLALGAVLAQSKGASIAEGAGAMNITGSS
                     VSLTPSPEPDHQAPEPNPVGPTTWATGISAIPVSNPAVPANIPAFTEADVRNYNSLHH
                     AHGLAMVRSLVAPTVVQVTFLTNQALRARAPGLAASVPDDRLMCYVKYQGAFVSSTSP
                     GTTVPYVVEIFDAHTGNLMSVGLDPADR"
BASE COUNT          793 a         1159 c         1206 g          660 t
ORIGIN      
        1 atgcgcgagg tcattctcga cctccagcaa caactcgccg agatccggcg ggaattagat
       61 attgacattt agggtatact ggccttgagt agcccatcac gaggggccgg gtataccagg
      121 taagggaaac aacgcatgcc aagcaatggg tttacggagc gggcgcagga agcgctgcgc
      181 aacgcccagg aaatcgtgca gcaaaagcgc catagccagc tcgatgtcga gcacattttg
      241 ctggccctgc tcaagccgcg cgatggcctg atctcgaaga ttatcgaaaa gctgaacggc
      301 gacccgcggg cgctggcccg ccggctcgac gaccagttga acaccagccc gcggctctat
      361 tcgagctaca gcggcctggc gcagatccac atcagcatgc gcgcacagcg cgtggtcagc
      421 gccgccgccg aagaggccgc caagctgaac gacgaatata tcggcgtgga gcacctgttc
      481 ctcgccatca ccgccgagcg cggcggcacc gccgcccgcc tgctgagcga gatcaacatc
      541 gaccaggcga gcgtctatac cgccctgcag gagatccgcg gcaacgcccg catcaccgat
      601 cccggcgcgg atggccgcta ccaggcgctg gagcgctaca gccacgacct gacccgcatg
      661 gcccgcgaag gccagttgga cccggtggtg ggccgcgagg ctgagatccg gcgcgtcatg
      721 caggtgctct gccggcgcac caagaacaac ccggtgctca tcggcgagcc gggcgtgggc
      781 aagacggcca tcgtcgaggg cctggcccag cagatcgcga ccggcgatgt gccggccctg
      841 ctgcgcaaca agcgcgtgct ggcgctcgac atggggtcgc tggtggcggg cagccgcttc
      901 cgcggcgagt tcgaggagcg cctcaaggcc gtcatggacg aggtgaagca ggccagcaag
      961 gagatcattc tctttattga cgagctccac accgtcatcg gcgcgggcgc ggcggaaggc
     1021 gcgctcgacg cggccaatat gctcaagccg gccctggccc gcggcgagtt gcagtgcgtc
     1081 ggcgcgacca cgctggacga gtatcgcaag cacatcgaga aggatgccgc cctggagcgg
     1141 cgcttcgcca cggtcttcgt ggaggagccg acggtcgaag ctacgctggc gatcctgcgc
     1201 ggcctgcgcc cgcgctacga ggagcatcac ggcctgaaga tcaccgacag cgcggtggac
     1261 gcagcggtgg aactgtccaa ccgctacatc caggaccgct tcctgcccga caaggccatc
     1321 gacctgatgg acgaggccgc gtcgaaggtg cgcctggagg tcttcagtct gcccgccgac
     1381 gtgcgggagc ttgaagcgct catgcacgat ctcgaagagc agatggaaga ggccggccag
     1441 cgccaggact acgcggaagc cgcccggctc aaggccgaac tgctgctgca cgaggagact
     1501 tacagccaga agcgcgacgc cttcttcgcc gagcacaaca tcgacgagat cgtggacgag
     1561 gaagacgtgg ccgatgtggt ggcccgctgg acgggcatcc cggtcaagcg catgctggcc
     1621 gacgagatgc agaagctggt cgacatggaa gagcacctgc acaagcgggt gatcgggcag
     1681 gacgcggcga ttgccgccgt ctccgatgcg atccgccgcg cgcgcgccgg gctgaaagac
     1741 ccgcgccgcc cgatggggtc gttcatcttc gtcggcccga ccggcgtcgg caagacggag
     1801 ctggcgaagg cgctggccga gttcatgttc gacgacgaga acgccatcgt gcgcgtggac
     1861 atgagcgagt acggcgagcg gcacacggtc gcccggctca tcgggtcgcc gcccggctat
     1921 gtcggctacg acgagggcgg gcagttgacc gaggcggtgc gccgccggcc ctaccaggtg
     1981 gtcctcttcg acgagatcga gaaggcgcac ccggaggtgt tcaacgcgct gctgcaagtg
     2041 ctggacgatg ggcggctgac cgacggccat ggccgcacgg tcgacttccg caacacgctg
     2101 atcatcctga ccagcaacgt gggcacgcag ttcatgccgc agtacggcgg cctgggcttc
     2161 cgcacccccg gcgcggccag ggatacgcgc aacgaggaga tcgaaacgcg ccaccgcgtc
     2221 gagcgggcca tgaaggaatc cttccggccc gaggtcctga accgcatcga cgacacgatc
     2281 ctgttcagcg cgctgtcgct ggacgaactg cgccagatcg tggacatcca ggtccgcgaa
     2341 ctgaccgagc gcatgggcgt gcagcaactg cgcctggaac tgaccgacga ggcccgcacc
     2401 tggctggccg agcgcggtta cgaccggacg tttggcgctc gcccgctcaa gcgcctgatc
     2461 cagcgcgagc tggagacgcc gatcagcaag ctgctgctgc gcggcgacct gaaggcgggt
     2521 cagggtatcc tggcgaccgt ggccgaagac ggcagccggc ttatgccgga agtcgtcggc
     2581 gaacccgcgg tggccctccc ggtcacgcag gacgccgacg cacaggtcgg gtagctgtca
     2641 gccgtcagct atcagctgtc aggtagataa tagaaaggcc accggggttg cccggtggcc
     2701 tttttctttt tgatccaacc gcagtggggc cgagtaacgg ccctgtcgca gctccgtatg
     2761 gtatactcag tccggccccg taccgaaagc atactaccta tcagccgggt ccaaaccaac
     2821 cgacatcaag ttgccggtat gtgcgtcgaa aatttcaact acatagggta ccgtggtgcc
     2881 ggggcttgtg ctgctgacaa aagcgccttg gtatttgaca tagcacatca atctatcgtc
     2941 aggaacactg gcggccaggc ccggagctct tgcacgaagc gcttgattgg taagaaatgt
     3001 aacttgcaca actgtgggtg ctactaacga ccggaccata gcaagtccat gtgcatgatg
     3061 caagctatta tagttccgta catctgcctc cgtaaaggcg gggatattag ctggcacggc
     3121 aggattgctt accggaatag cggaaatacc cgtggcccaa gtcgttggcc caacagggtt
     3181 cggctcggga gcttgatgat ctggttctgg tgaaggagtc aaagatacag aagatcccgt
     3241 tatattcata gctcctgcgc cctcagctat acttgctccc ttggactgag caagcaccgc
     3301 tcccagggca agcagcacta ccgccgccat gacaaacaag gctagtctat atgaattcat
     3361 ataatggtac tccttctagc ctgatataga acgactgacc ggcgcgagat ccaaccgttt
     3421 tctaacatgt acaattagtt ttgaaagtgc ctccaggcca actgcaagct agcacttcga
     3481 ggtttcgttt tatttgtgtt ccagtttgga ttctgcattg gccgatactt caattaacag
     3541 gttgccacga ccctcacgat tgcgaggatg cggcactcag cacccggtaa atcagttgga
     3601 caggcaaggc gggtactcgt ggcgatccgt agcgcatata ttataccacg ttctggacct
     3661 ttttaaaaca tttatatatc gagtatagta ataatgtctg gagcgcttgc tactaaggag
     3721 gcaatccgtg aaaattcagc aatgggctgg gcgtgttata gtagccgcgc tgattgtgcc
     3781 agtgattcta gcggttgcct ttgtagcaag taccacat
//
LOCUS       DASUYC010000345         3073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_50911,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000345 DASUYC010000000
VERSION     DASUYC010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50911"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VF276_05660"
     CDS             complement(<1..253)
                     /locus_tag="VF276_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6606387.1"
                     /translation="MAQPAAQILVVDDEAPIRTTLTALLERRGYEVTAAATGEDALAL
                     IHQRPFDLLLLDLQLPGINGMDVATRARDRQPDAQIIILT"
     gene            complement(282..2039)
                     /locus_tag="VF276_05665"
     CDS             complement(282..2039)
                     /locus_tag="VF276_05665"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6606388.1"
                     /translation="MVKSVHKRAIPPGEAGADSLSLPPSGTVPTAAWFSALLESAPDA
                     IVIVDDTGQIVLVNSRSEELFGYDRAELTGRSIELLVPDQLRAVHAQDRAHYYAHPQT
                     RPIGSGRILLARRKDGSEFSAEISLSPVQTENGLLVMSVIRDISERRRAAEELEQQVQ
                     RRTAHLNALLQVSSELLATRSRDAMLERALIHALALVPQAARAAIYLYDPETDRLALR
                     AGLGAMPITLPIDPGILGAAFTSRRTYRASSVATVLDRARAEGVDTPLISVLDPEHPP
                     SGVLAIPLIARDQAIGVLALVRDSGEGPFAAEAQATVEGLANLTATAILEERSRHEAA
                     ALSSQLADLEEQQRTMAARLTTVEAGMLQTARLAAVGELAAAVAHEINNPLYAARNCL
                     YLLEEDLPPALRDSPYMTMARDQLARIATIIERMRDFYRPARGELAPYNLNRGLEETL
                     ELAALNLRKLPIKMIFTPDPALPPVVCDSDQLRQVFLNLILNAIDAMPEGGTLTVRTE
                     AGPTMALVEIADSGIGIPPEVRERLFEPFFTTKPSGTGLGLSISAHIVTQHGGQILVE
                     SQEGQGSTFRVVLPYHPDI"
     gene            2194..2583
                     /locus_tag="VF276_05670"
     CDS             2194..2583
                     /locus_tag="VF276_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6606389.1"
                     /translation="MPLHILIVDADRTAAQVTRAAVARSLPAATVTVTTDPAHPWMRT
                     PPEPLDVLIIDPPPRPPAGTQLIRRAQAIWPGVLVIVLASAPTPALRRDLRALAVDVY
                     LEKPTLLPVVISALHTALQRPRRACVD"
     gene            2806..>3073
                     /locus_tag="VF276_05675"
     CDS             2806..>3073
                     /locus_tag="VF276_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606390.1"
                     /translation="MRVLVINAIDGRLDQLQAVLDHQQRDIAAVFVLGNLTPAAARRQ
                     AYQAQIRSGQIPAPVIEALDRESRLTEQSYANVFTLLGNLGVPVY"
BASE COUNT          448 a         1032 c         1054 g          539 t
ORIGIN      
        1 cggtgaggat gatgatctgg gcgtcgggct ggcggtcgcg ggcgcgcgtc gccacatcca
       61 tgccgttgat gccgggcaac tggaggtcga gcagcagcag gtcgaagggc cgctggtgga
      121 tcagcgccag cgcgtcttcg ccggtggccg cggcggtgac ttcatagccc cggcgctcca
      181 acaaggcggt caacgtcgtg cgaatcggcg cctcgtcgtc gacgaccagg atctgcgcgg
      241 caggctgggc catagcttct cgtactcctt ccgggcgacg ttcaaatatc ggggtgatac
      301 ggcaacacga cgcggaaggt gctgccctgg ccttcctggc tctcgaccag gatctgcccg
      361 ccgtgctggg tgacgatgtg cgcgctgatc gagaggccca accccgtacc gctgggcttg
      421 gtggtgaaaa acggctcgaa cagccgctcg cgcacctccg gcgggatgcc gatcccgctg
      481 tcggcgatct cgaccagggc catggtcggc ccggcctccg tccgcaccgt cagtgtgccg
      541 ccctcgggca tcgcgtcgat ggcgttcaag atcaggttca ggaagacctg gcgcaactga
      601 tcgctgtcgc aaaccaccgg cggcagggcg gggtcggggg tgaagatcat cttgatcggc
      661 agcttacgca aattgagcgc cgccagctcc agcgtctctt ccaggccccg gttcagatta
      721 tacggcgcca gctccccgcg cgccgggcgg tagaagtcgc gcatgcgctc gatgatggtg
      781 gcgatgcggg ccagttggtc gcgcgccatg gtcatataag gcgagtcccg tagcgccggg
      841 ggcaggtctt cttccagcag gtagaggcag ttgcgcgcgg catagagcgg gttgttgatc
      901 tcgtgggcca cggcggcggc cagttcgccc acggcggcca ggcgcgccgt ctgcaacatg
      961 cccgcttcga ccgtcgtgag ccgggcggcc atggtgcgct gctgctcctc caggtcggcg
     1021 agttggctgg acagggccgc cgcttcgtgg cggctgcgct cttcgaggat ggcggtggcc
     1081 gtgagattcg ccaggccttc caccgtggcc tgggcctcgg cggcaaacgg cccctcgccg
     1141 ctgtcacgca ccagagccag cacgccgata gcctggtcgc gggcgatcaa cgggatcgcc
     1201 agcacgccgc tgggcgggtg ttccgggtcg agcacgctga tcagcggcgt gtccacgccc
     1261 tcggcgcgcg cgcgatcgag cacggtggcc acggaactgg cgcggtaggt ccggcggctg
     1321 gtgaaggcgg cgcccaggat gccgggatcg atgggcaagg tgatcggcat ggcgcctagc
     1381 ccggcgcgga gcgccagccg gtcggtctcc gggtcgtaca ggtaaatggc ggcacgcgcc
     1441 gcctggggca ccagggccag cgcatggatc aacgcgcgct ccagcatggc gtcgcggctg
     1501 cgcgtcgcca gcaattcgct gctcacctgg agcagcgcgt tgagatgggc cgtgcggcgc
     1561 tgcacctgtt gctccagctc ttcggcggcc cggcgccgct cgctgatgtc gcggatcacg
     1621 ctcatcacca gcagcccgtt ctccgtctgg acggggctca ggctgatttc ggcggagaac
     1681 tcgctgccgt ctttgcgccg ggccagcagg atgcgcccgc tgccgatggg ccgcgtctgc
     1741 gggtgggcgt agtagtgcgc gcgatcctgg gcgtgcaccg cacgcagttg atcggggacc
     1801 aggagctcga tggaccggcc ggtcagttcg gcgcggtcat agccgaagag ttcttcgctg
     1861 cggctgttga ccagcacaat ctgcccggtg tcgtccacga tgacgatggc gtcgggcgcc
     1921 gattccagca gggcgctgaa ccaggccgcg gtgggcacgg tgccggacgg cggcaggctc
     1981 agggagtccg cgcccgcctc gcccggcggg atagcgcgtt tatgcactga cttaaccata
     2041 acctgctccg tttcgggcgg ggtgccgttc gtccgccccc tgtatcatac aggttcgcgg
     2101 cgcccggatc aaatggcctg tggtggtgag cttgctaccc acggttagcg gatgtttcac
     2161 acccgtgccg gtagaacctc ttatactagt gctatgcctc tccacatttt gatcgtcgat
     2221 gccgatcgta ctgccgccca ggtgacgcgc gcggcggtgg cccgcagcct gcccgccgcg
     2281 accgtgaccg tgaccacgga cccggcgcat ccctggatgc gcacgccgcc ggagccgtta
     2341 gacgtgttga tcatcgaccc gccgccgcgc cccccggcgg gcacccagct tatccgccgg
     2401 gcacaagcga tctggccggg ggtcctggtg atcgtgctgg cctccgcgcc caccccggcg
     2461 ttgcggcgcg atttgcgcgc cctggcggtg gacgtgtacc tggaaaagcc cacgctcttg
     2521 ccggtcgtga tcagcgccct gcacaccgcg ctgcaacgcc cccgccgcgc ctgtgtggat
     2581 tagctcgctc cggcggttgg gcgcgacccc atcggaggca cttgcatgcg tgcggtatac
     2641 tattggtaag gtatgacggt aaggttgggg gatgagccgc gcacccgctc atctctatcc
     2701 cgcttgtgtt actaaccgcg gctgtttgaa tccacgcccc atatccggcc atggcactca
     2761 actctcgcgt cccgcgccgg gagggccaat ccctggaggt atgccatgcg cgtgctcgta
     2821 atcaacgcca ttgacgggcg gctcgaccaa ttacaggccg ttctcgacca ccaacagcgc
     2881 gatattgccg cggtgtttgt tctcggcaat ctcacccccg ccgccgcccg gcgccaggcc
     2941 tatcaggcgc agatccgcag cggccagatc ccggccccgg tgatcgaagc cctcgaccgc
     3001 gagagccgcc tcaccgagca aagctacgcc aatgtgttta ccttgctcgg caatctcggc
     3061 gttccggttt atc
//
LOCUS       DASUYC010000346         4193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1597486, whole genome shotgun sequence.
ACCESSION   DASUYC010000346 DASUYC010000000
VERSION     DASUYC010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1597486"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="VF276_05680"
     CDS             complement(<1..720)
                     /locus_tag="VF276_05680"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX6606391.1"
                     /translation="MATKATTRPAAMPVTQAKTVEREPMYYVIMVIRYIVLILMTIIF
                     LGPFVMALLGSFKSTAEVVAWPPTFLPKEWRFQNYVDVWNVLPDENGNSYFPRWILNS
                     FIMSASIVVAQLFFCSLSAFAFARLRMPGKGIVFAAMLSTMMIPGMVLMIPKYQILNA
                     LHLVNTYWAIIIPGVVSAAMIFMLTQFFQSIPKDLDEAAQIDGAGIFTTYWRVILPLA
                     KPALVTLPLLTFQGVWNDFLNM"
     gene            complement(756..1685)
                     /locus_tag="VF276_05685"
     CDS             complement(756..1685)
                     /locus_tag="VF276_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX6606392.1"
                     /translation="MATQVQAAPSPKAPGMSRKASEALHAYLFVAPWIILSIIFTFGA
                     MIYVFYLSFTNLRLFNTPELIGLTNYTSVLQNKLFWTAVQNTLEYAVVVTFFQTWLAL
                     ILAVILNAKIPGKQFFRIGWYIPSVTSSVVISLVFVWVFFKQGILNYVLVTLFGWTGF
                     QPVAWLTDAGAHTALPALMLLNISTTAPTFMLYFLAALQDIPEEIYEAASLDGAVGVA
                     RFFKITVPMLRPIIFLVVVLGTIGTLQLFDQALIMTAGGPLNQTTTINLLIYQAAFTN
                     SEMARASAMAFILFVLIFVLFLVQRRFLGNTNQ"
     gene            complement(1788..3293)
                     /locus_tag="VF276_05690"
     CDS             complement(1788..3293)
                     /locus_tag="VF276_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX6606393.1"
                     /translation="MVKSTRWMFVIALLVISSMLLSACGGESPTATTGTGGGAAAATD
                     TPAAAAPAATDTPAAAAGAATDTPAAAGAATATEAMAATPASGGASSSTFDPSAYKKN
                     AVEDGAVLRVSSWGDTSEQQVNRDAIARFNQVYPNVQVNYEPQPTDYQTKLKAQVKGG
                     SEPDVFYIDPGLAFELMPAGILMDLGPALQEVGRSKDDYFPSLINTFILDNKVYGLPK
                     DFGSLAVFYNTDMLAKTGATEPKEGWTQDDFKNFVAKSVQGTDPNSKIYGTSVDPDPE
                     RWMAFALANGGKILDNNKCAINSDIGVSTLDWWYGMYKDKTATIPADVGAGWSGEAFA
                     KQRIASTVQGGWLIPFMADPKGGFNNVKYDVAPLPLGKSGQPADLLFFNAWGASAKSK
                     YPKAAAALALFLASRENEGAILQTGFALPTLKGFENDPFFQGTGVVNKASKLLYATAQ
                     YGVPDYYGGINDPKIKKALADATQRVYANQQSSKDALDQACQEIDPLLAGP"
     gene            complement(3784..>4193)
                     /locus_tag="VF276_05695"
     CDS             complement(3784..>4193)
                     /locus_tag="VF276_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606394.1"
                     /translation="ALAEFRYGWPDKGLWYLERMAELCGYYMPGAIPEFVGPEACFLQ
                     AWSSAAYNWLLVQGFFRLNPDPARGVVTVQPQLPTGWDFLEVRNLTIWGVPCRLRLQR
                     TASGIDFKAETGAPGTPPTFEVAGDAPVPVGFV"
BASE COUNT          842 a         1272 c         1339 g          740 t
ORIGIN      
        1 catgttcagg aagtcgttcc acacgccctg gaaggtcagc agcggcagcg tcaccagcgc
       61 cggtttcgcc agcggcaaga tcacccgcca gtaggtcgtg aagatgcccg cgccgtcgat
      121 ctgcgccgcc tcatccaggt ctttgggaat gctctggaag aactgggtca gcatgaagat
      181 catcgccgcg ctcaccacgc cggggatgat gatggcccag taggtgttga ccagatgcag
      241 cgcgttcagg atctggtact tggggatcat caataccatg ccggggatca tcatcgtgga
      301 cagcatggcc gcgaagacaa tgcccttccc cggcatgcgc aggcgggcaa aggcaaaggc
      361 ggatagcgag cagaaaaaga gctgcgccac cacgatgctc gccgacatga tgaagctgtt
      421 caggatccac cgcgggaagt acgaattgcc gttctcgtcc ggcagtacat tccagacatc
      481 gacgtagttc tgaaaccgcc actccttggg caggaaggtc ggcggccagg ccaccacctc
      541 ggcggtgctc ttaaacgacc ccagcagcgc catgacaaac gggccgagga agatgatcgt
      601 catcaagatc agcacaatgt agcggatgac catgatgacg tagtacatcg gctcgcgctc
      661 aacggtcttc gcttgcgtga ccggcatcgc cgcggggcga gttgtcgctt ttgtcgccat
      721 cgttaagcct ccatcagaac cccagggggc gcaaactact ggttcgtgtt gccgaggaac
      781 cggcgttgca ccaggaacaa aacgaagatc agcacgaaca ggatgaacgc catggccgag
      841 gcccgcgcca tttccgagtt cgtaaacgcc gcctggtaga tcaataagtt gatggtggtc
      901 gtctggttga gcgggccacc cgccgtcata atcagcgcct ggtcgaagag ttgcagcgtg
      961 ccgatggtgc cgagcaccac caccaggaag ataatcggcc gcagcatcgg gacggtaatc
     1021 ttaaagaacc gggccacgcc gacggcgccg tccaggctcg cggcctcgta gatctcctcc
     1081 gggatgtcct gcagggccgc caggaagtag agcatgaacg tcggcgccgt agtcgagata
     1141 tttaacagca tcagcgccgg cagcgccgta tgcgcgcccg catcggtcaa ccaggccacg
     1201 ggttggaagc cggtccaccc aaacagcgtc acgaggacgt agttcaggat gccctgcttg
     1261 aagaagaccc acacgaagac gagcgaaatc acgaccgacg acgtgacgct gggaatgtac
     1321 cagccgatgc ggaagaactg cttaccggga atcttggcgt tgaggatcac cgccaggata
     1381 agcgcgagcc acgtctggaa gaaggtgacg acgacggcat attccagcgt gttttgcacc
     1441 gccgtccaga acagtttgtt ctgcaagacc gaggtatagt tggtgagacc gataagctcg
     1501 ggcgtgttga acaggcggag attcgtaaag ctcaaataaa agacgtagat catcgccccg
     1561 aaggtgaaga tgatcgagag gatgatccag ggcgcgacaa agaggtaggc gtgtaaggct
     1621 tcggaagcct tacgcgacat gcccggtgcc ttggggctgg gggccgcctg gacttgagtt
     1681 gccataacgc tcctcctcgg cggcccgcgg cgccaggaag cgtcgcgggc cggtaactac
     1741 cgggatgaaa gcgcgccgcc tgctcgatgc agcgcggcgc gcgggtctca cggccccgcc
     1801 agaagcggat cgatctcctg gcaggcctgg tctagcgcgt ctttcgacga ctgctggttc
     1861 gcgtacaccc gctgcgtggc gtcagccagc gccttcttga tcttcgggtc gttgatgccg
     1921 ccatagtagt cgggcacgcc gtactgcgca gtcgcgtaga gcagcttgga tgccttgttg
     1981 accacgccgg tgccctggaa gaagggatcg ttctcgaagc ccttcagcgt cggcagcgcg
     2041 aagccggtct gcaggatcgc gccttcgttc tcccggctgg cgaggaacag cgccagcgcg
     2101 gcggcggcct tcgggtactt cgatttggcc gaggcgcccc aggcgttgaa gaagagcagg
     2161 tcggcgggct ggccgctctt gcccagcggc agcggcgcca catcatactt cacgttgttg
     2221 aacccgccct tgggatcggc catgaacggg atcagccaac cgccctggac ggtcgaggcg
     2281 atacgctgct tggcaaaggc ttcgcccgac cacccggccc ccacgtcggc ggggatcgtg
     2341 gccgtcttat ccttgtacat gccgtaccac caatcgagcg tcgagacacc aatgtcgctg
     2401 ttgatggcgc acttgttgtt gtcgagaatc ttgccgccgt tcgccagggc gaaggccatc
     2461 cagcgctcgg gatcgggatc gaccgaggtg ccgtagatct tgctgttcgg gtcggtgccc
     2521 tggaccgact tggcgacgaa gttcttgaaa tcgtcctggg tccagccttc cttgggctcg
     2581 gtcgcacccg tcttcgccag catatccgtg ttgtagaaca cggccagcga accgaagtcc
     2641 ttcggcagcc catagacctt gttgtccagg atgaacgtat tgatcaggga cgggaagtaa
     2701 tcgtccttgc tgcggcccac ttcctggagc gccgggccga gatccatcag gataccggcg
     2761 ggcatcagtt caaaggccag accggggtca atatagaaaa cgtcgggttc cgagccgccc
     2821 ttgacctggg ccttgagctt ggtctggtaa tcggtgggct gcggttcgta gttgacctgg
     2881 acgttcgggt agacctggtt aaagcgggcg atggcgtcgc ggttcacctg ctgctcggag
     2941 gtatcgcccc aactggagac acgcaggaca gcgccgtctt caacggcatt cttcttatag
     3001 gcactgggat cgaaagtgga actggacgcg ccgccgctgg ccggggttgc ggccatggct
     3061 tcggtggcag tcgccgcgcc cgccgccgcc ggggtatcgg tggccgcccc tgcggcggct
     3121 gccggggtat cggtggcggc gggtgccgcc gcggcgggag tatcggtggc cgctgccgcg
     3181 ccgccgcccg tgcccgtggt cgccgtggga ctttcgccgc cgcacgcgct gagcagcatc
     3241 gagctgatca cgagcagcgc gattacaaac atccatcgag tcgatttgac catttcggct
     3301 ttcctcctcc tgcgaaccac gctctggtgg ccgcatcgct gattagactt cacggggtaa
     3361 agccccagcc gcactgatgg atcgatagag tgttagaaga gccattcctc ctttcttgcc
     3421 cggtgccgcg caaatggccg cctgggtgtt gccacccaat ccgcagtggt gcgcacagac
     3481 tagatagccg aatgtggtcg ccacgtcgcc caaccacttg cgaacaacgg gtgaaagccg
     3541 atagacaagc ttaaccgacg actggagcaa aatctgtgcc gtaaagctgt ccaccggcgc
     3601 gagtgccgcc atgccgggat agcccgtaaa cccgcgcaac gggtacatgg cgccggtgca
     3661 agcactacaa cgaaaaacta tccaaaacgt tttggatgat tcggataata gcaccaaaaa
     3721 cgaagtctgt caatgggtag cgcgggcgga gatgacgact gaggaactgc gaaccgcggg
     3781 agcttacacg aagcccaccg gtaccggcgc atcgccggct acctcgaagg tcggcggcgt
     3841 gcccggcgcc ccggtttcgg ccttgaagtc gatgccggat gccgtccgct gcaagcgcaa
     3901 gcggcagggc accccccaga tggtcaggtt gcgcacctcc aggaagtccc aaccggtggg
     3961 caactggggc tggacggtga cgacaccccg cgccgggtct gggttcaggc ggaagaagcc
     4021 ctgcaccagc agccagttgt aggcggcgct ggaccaggct tgcaggaagc acgcctccgg
     4081 ccccacgaac tcggggatcg cgcccggcat gtaatacccg cagagctcgg ccatgcgctc
     4141 caggtaccag aggcccttgt cgggccagcc atagcgaaac tcggccagcg cga
//
LOCUS       DASUYC010000347         5312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_517009,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000347 DASUYC010000000
VERSION     DASUYC010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517009"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..808
                     /locus_tag="VF276_05700"
     CDS             230..808
                     /locus_tag="VF276_05700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6606395.1"
                     /translation="MRMWRDDREKQALFEEVALAHLSGLYYAALRLTGRPTDAEDLVQ
                     ETYTRGYAAFDRFTLGTNARAWLYRIMNNLFLNQVSRAEARRTRSFSATSPADIAAWE
                     APAADPAEILLDRTLDARLSAALRELPPDFRSALVAVDVGGFSYEEAAGMLGCPVGTV
                     RSRLYRARSLLRARLTEGMPELPANDLSGGAP"
     gene            805..1626
                     /locus_tag="VF276_05705"
     CDS             805..1626
                     /locus_tag="VF276_05705"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX6606396.1"
                     /translation="MTPCPEPRDLLSAYVDGELDPATRRTIARHLEECARCRADVEGQ
                     RQVKRFLAPGAALAPPVPADLAGRVRGRAYAGVATPPARRFGLPRWRPSRRWALAGAL
                     AVVVLVLALGLGTVALNLFDRSQAATVAMMSMQDHEMAEQMGTVPHTLPGDMAAVRAQ
                     LATTMGMSVSAPAEMPPGYHFAGGRALMLGDTHGAHLAWMAGDSMLSLYQAQDPGGSP
                     PGSWHTVQQGDRTYWMDTGGSDHAVFWRSGGMVYVLVGRLPDASLLDVAHSVDPA"
     gene            complement(1756..2745)
                     /locus_tag="VF276_05710"
     CDS             complement(1756..2745)
                     /locus_tag="VF276_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606397.1"
                     /translation="MPQMTKARRAIGVMAGGLLVTGLAGMTLLGQAGAAPRPAPSADA
                     FADPAFEQVWGRTDFLVAQGQAARSWYWGPAAGRSVYEPYDEGSGRLHLVQYFDKSRM
                     EINDVTADRNSKWFVTNGLLTVELVSGRVQFGNTRWVPKTGGPAPIPVAADADDANAP
                     TYASFTNLANTILGDHKAPDRSGQAVQETVNRAGQVAPAPDKAAYGVRYAHFEPLTGH
                     NVAGVFWDFLNARGPVRENGATVTAMLSDPWFFTSGLPISEAYWARAKIANTQHDVLI
                     QLFERRVLTYIPDYDPAWRVQMGNIGQHYVQWRYPSGLPAPVPTAPALLQLTH"
     gene            complement(2759..3469)
                     /locus_tag="VF276_05715"
     CDS             complement(2759..3469)
                     /locus_tag="VF276_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX6606398.1"
                     /translation="MYKGLSISVVIPCFNEEKGIGTVLQGMPDYVDEVIVVDNNSADG
                     TARIAEAHGARVVHEKRKGYGSAYQAGLPQARGDIIATLDGDGTYPATEIAPIIDWML
                     ARKVDFVSASRFPLQKREAMRRRNWLGNMILTWTTRLLWLTWIHDSQSGMWIFRREML
                     RHLRLTSLGMPFSEEIKIEAMTNRRYRFAEYPIRYEERIGESKLFPFQNGVENLLFLF
                     KLRLGRRQHARPLPGSRD"
     gene            3727..4716
                     /gene="galE"
                     /locus_tag="VF276_05720"
     CDS             3727..4716
                     /gene="galE"
                     /locus_tag="VF276_05720"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010347279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEX6606399.1"
                     /translation="MLVLVTGGAGYIGSHTVRVLRERGYDVLVLDSMEFGHQEAIGDT
                     PLVRGNIADGALLDRLFAEHPIEAVIHFAAYKAAGESMEQPGRYFENNVCGTLVLLEA
                     MTRAGVRRFIFSSSCSVYGTPDVLPVSEANPLHPESPYAETKRMVEQMLHWFDVVHGL
                     RFVSLRYFNAAGAALDGSIGEDPTFTLNLIPLVMKAALGKIPAVQVFGTDYPTPDGTA
                     IRDYIHVLDLADAHVKALEYLMAHDRSEFVNLGTGHGSSVQEIIDLARKVSGVDIPVK
                     YTGRRPGDPVAIWADNSKARTLLGWQPQYDLETILRSAWRWHSTHPNGFGPAG"
     gene            4887..>5312
                     /locus_tag="VF276_05725"
     CDS             4887..>5312
                     /locus_tag="VF276_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX6606400.1"
                     /translation="MTTELVGTETQPQGLDNYKYGFHVPENYVFKAKRGLTREVVAEI
                     SEMKGEPQWMRDFRLRALEMFLRKPMPTWGGDLSGINFDEIFYYIKPTQGQGKTWEDV
                     PAEIKDTFERLGIPEAERKYLAGVGAQYESEVIYHSIRED"
BASE COUNT          863 a         1746 c         1807 g          896 t
ORIGIN      
        1 tgcccctgac cgccacccat gcccgccgcc ccatgcggcg ccccgcccgc ccgatcctgc
       61 agatcgggcg ggcactgctt tcgcccctcg actacgcccg gcgggaactt tcctcggcct
      121 tcccctgttg tctataatag agaaggcgcc ggggaagcat ccggcgcgat tctcgcccat
      181 tgtgatccac gagtgctacg cggcacagga gttgaaggaa cgccggccca tgcggatgtg
      241 gcgcgacgac cgcgaaaagc aagcgctgtt tgaagaggtc gcgctggcgc atctctccgg
      301 gctgtactat gccgccttgc ggctgaccgg ccgccccacc gacgccgagg atctggtgca
      361 ggaaacctat acccgcggct acgccgcctt cgatcgcttt accctgggca ccaatgcgcg
      421 ggcctggctc tatcgcatta tgaacaacct ctttctcaac caagtcagcc gcgccgaggc
      481 ccgccgcacg cgctctttca gcgccacctc gcccgccgac atcgccgcct gggaggcgcc
      541 tgccgccgac cccgccgaga tcctgctgga ccgcacgctc gacgcccggc tcagcgcagc
      601 cctgcgcgaa ctgccgcccg atttccgcag cgccctcgtg gccgtcgatg tgggcggctt
      661 ttcctatgaa gaagccgcgg ggatgctggg ttgcccggtg gggacggtgc gctcgcggct
      721 ctaccgggcg cgcagcctct tgcgcgcccg gctgaccgag ggaatgcccg aactgcccgc
      781 aaacgacctg agcggaggcg cgccatgaca ccttgccccg agccgcgcga tctcctcagc
      841 gcctacgtgg acggcgaact cgacccggcc acccggcgga ccatcgcgcg gcacctcgaa
      901 gagtgcgccc gctgccgggc cgacgtggaa ggccagcgcc aggtcaagcg gttcctggcc
      961 cccggcgcgg ccctcgcgcc gcccgtcccc gccgatctcg cggggcgcgt gcgggggcgc
     1021 gcctatgccg gcgtcgccac gcccccggcg cgccgcttcg ggttaccgcg ctggcggccc
     1081 tcgcggcgct gggcgctggc gggcgcgctg gccgtcgtgg tgttggtcct cgcgctgggc
     1141 ctgggcacgg tcgcgctcaa cctgttcgac cgctcgcaag cggccacggt cgccatgatg
     1201 tcgatgcagg accacgagat ggccgagcag atgggcaccg tgccgcacac attgccgggc
     1261 gatatggcgg cggtgcgcgc gcaactcgcc accaccatgg gcatgagcgt ctcggcgccc
     1321 gccgagatgc cgcccggcta ccactttgcc ggcgggcggg cgctgatgct gggcgacacg
     1381 cacggtgccc atctggcctg gatggcgggg gattccatgc tctcgctcta ccaggcgcag
     1441 gatccgggcg gcagtccgcc ggggtcctgg cacacggtgc aacagggcga tcgcacgtac
     1501 tggatggaca ccggcggcag cgaccacgcc gtgttctggc gcagcggcgg catggtgtac
     1561 gtgctggtgg gccggttgcc cgacgcgagc ctgctcgacg tggcccactc cgtcgatccg
     1621 gcctgatgca ggttccggcg acctgttagg ctacgaatac gctgacgacc aacgagtacg
     1681 gcgcgataaa tcgcgcaact acactcgatc agggtgggcg cgtagcgtgc gcccaccctg
     1741 tcgccggcta tcggatcagt gcgtgagttg cagcagcgca ggtgccgtgg gcaccggggc
     1801 gggcaggccg ctcgggtagc gccactgcac gtagtgctgg ccgatgttgc ccatctgcac
     1861 gcgccaggcc gggtcgtagt cggggatgta ggtgagcacg cggcgctcga aaagctggat
     1921 cagcacgtca tgctgggtgt tggcgatctt ggcgcgcgcc cagtaggctt cgctgatcgg
     1981 caggccgctg gtgaagaacc agggatcgct gagcatcgcg gtcacggtcg cgccgttttc
     2041 gcgcaccggg ccacgggcgt tgaggaagtc ccagaagacg cccgcgacgt tgtggccggt
     2101 cagcggctcg aagtgcgcgt agcgcacgcc gtaggcggcc ttgtcgggag cgggcgccac
     2161 ctggcccgcc cggttcaccg tctcctgcac cgcctggccg ctgcggtcgg gcgctttgtg
     2221 gtcgcccagg atcgtgttgg caaggttggt gaagctggca taggtcggcg cgttggcgtc
     2281 gtcggcgtcg gcggctacgg ggatgggcgc cgggcccccc gtcttgggca cccagcgcgt
     2341 gttgccgaac tgcacccggc ccgacacgag ttcgacggtg agcaggccgt tggtgacgaa
     2401 ccacttggag ttgcggtccg ccgtcacgtc gttgatctcc atgcggctct tgtcgaagta
     2461 ttgcaccagg tggagccggc ccgacccctc gtcgtagggc tcgtagacgc tgcggcccgc
     2521 ggcgggcccc cagtaccagg agcgcgccgc ctgcccctgg gcgaccagga aatcggtgcg
     2581 cccccacacc tgttcaaagg ccgggtcggc aaaggcgtcg gcggagggcg ccgggcgggg
     2641 cgcggccccg gcctgcccca gcagagtcat cccggccagg ccggtcacga gcagcccgcc
     2701 cgccatcacg ccgatcgcgc ggcgggcttt cgtcatctgt ggcatatgcg tcgtcctgtc
     2761 aatcgcgtga gccgggcagg ggccgggcgt gctggcgccg ccccagccgg agcttgaaca
     2821 aaaagagcag gttctccacg ccgttctgga acgggaagag cttgctctcg ccgatgcgtt
     2881 cctcgtagcg gatcgggtac tcggcgaacc ggtagcgccg gttcgtcatc gcctcgatct
     2941 tgatctcctc agagaagggc atgcccaggc tggtcagccg caggtggcgc agcatctcgc
     3001 ggcggaagat ccacatgccc gactgcgagt cgtggatcca ggtgagccag agcagccgcg
     3061 tggtccaggt gagaatcatg ttgcccagcc agttgcggcg gcgcatagcc tcgcgcttct
     3121 gcaaggggaa gcggctggcc gagacgaaat cgaccttgcg cgccagcatc cagtcgatga
     3181 tcggcgcgat ctcggtggcc ggataggtgc cgtcgccgtc cagggtggcg atgatgtcgc
     3241 cgcgcgcctg gggtagcccg gcctggtagg cgctgccgta gcccttacgc ttttcatgca
     3301 ccacccgcgc gccgtgcgcc tcggcgatgc gcgccgtgcc gtccgccgaa ttgttgtcca
     3361 cgacgatgac ctcgtcgacg tagtccggca tgccctggag cacggtgccg atgccctttt
     3421 cctcgttgaa acaggggatg actaccgaaa tggaaagccc tttgtacacg ccacccttct
     3481 tctgcgcggc ctgtggccgg tcgcggtgaa tgtccgatta taacacgatt gccgggccgc
     3541 atcgctagca cggatcttgc cgcgcaatgc ccgatgtaca gtataccaga aagcggctgt
     3601 ttgcttccgc cgttgccggc gcacggcgta cattgccggg atgggcaccg cagaggcgca
     3661 gagaacgcag agattttaat gatggtagtg aataggtgac aatcgattca agctgggagg
     3721 acacagatgc tggtattggt gacgggcggc gcggggtata tcggttcgca taccgtgcgg
     3781 gtcttgcgcg aacggggcta cgatgtgctg gtgctcgaca gcatggagtt cggccaccag
     3841 gaggcgattg gcgatacgcc gctggtgcgc ggcaacatcg ccgacggcgc gctgctcgac
     3901 cgactgttcg ccgaacaccc catcgaggcc gtgattcatt tcgcggcgta caaagcagcg
     3961 ggcgagtcga tggagcagcc gggccgctat ttcgagaaca acgtctgcgg cacgctcgtg
     4021 ctgctggagg ccatgacccg cgcgggggtg cggcgcttca ttttctcctc gtcctgctcg
     4081 gtctacggca cgcccgatgt gctgccggtg agcgaggcca acccgctgca cccggaaagc
     4141 ccctacgccg agaccaagcg catggtcgag cagatgctgc actggttcga cgtggtgcac
     4201 ggcttgcgtt tcgtcagcct gcgctatttc aacgcggcgg gcgccgcgct cgacggcagc
     4261 atcggcgagg accccacctt cacgctcaac ctgatcccgc tggtcatgaa ggcggcgctg
     4321 ggcaagatcc ccgcggtgca ggtcttcggc accgattatc ccacgcccga cggcacggcc
     4381 atccgcgact atatccatgt gctcgatctg gccgacgcgc acgtgaaggc gctggagtac
     4441 ctgatggcgc atgaccggtc cgagttcgtc aacctgggga cgggccacgg ctcctcggtg
     4501 caagagatca tcgacctggc ccgcaaggtc agcggggtgg atatcccggt gaaatatacg
     4561 ggccggcgcc ccggcgaccc ggtggcgatc tgggcagaca acagcaaggc gcggacgctg
     4621 ctgggttggc agccacagta cgatctcgaa acgatcttgc gctcggcctg gcgctggcat
     4681 tccacgcatc cgaacggttt cgggccggct gggtaatacc ggcgcaaccc gtcaaagggt
     4741 tttcacagat tcgcctgtgt aaatgttgac aaaggcgcgg gaaaagccta taatgtagac
     4801 gtatcaatat gcgtttactc ctggggcaag atcggtatgg accggtagga gtccttgtcc
     4861 cggcggtaga ggtgaaggag cacgcgatga caaccgaact ggtcggtacg gaaacccagc
     4921 cacagggcct tgacaattac aagtacgggt tccacgtacc cgagaattac gtattcaagg
     4981 ccaagagggg cctgacccgc gaagtggttg ccgagatttc ggagatgaag ggcgagccgc
     5041 agtggatgcg cgatttccgc ttgcgcgccc tcgaaatgtt cttgcgtaag ccgatgccga
     5101 cctggggcgg cgacctctcc ggcatcaatt tcgacgagat cttctactat atcaagccga
     5161 cgcagggcca gggcaagacc tgggaagatg tgccggcgga gatcaaggac acgttcgagc
     5221 gcctgggtat ccccgaggcc gagcgcaagt acctagccgg ggtcggcgcg cagtacgagt
     5281 ccgaagttat ctaccatagc atccgcgagg ac
//
LOCUS       DASUYC010000348         7619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1398281, whole genome shotgun sequence.
ACCESSION   DASUYC010000348 DASUYC010000000
VERSION     DASUYC010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 7619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7619
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398281"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="VF276_05730"
     CDS             complement(<1..490)
                     /locus_tag="VF276_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1445 domain-containing protein"
                     /protein_id="HEX6606401.1"
                     /translation="MTLETPSPRAATYDAAGLRAAIRAGAFTGPTAGQAPDSAQANLV
                     ILPAAEAAAFREFCELNPRPCPLLEMTAPGDPVPHLLAPDADLRTDLPRYRVYRDGVQ
                     VDERTDIRDLWRDDLVAFLIGCSFTFESALLAAGVPVRHIELCSNVPMYRTNQPCTPA
                     GRF"
     gene            complement(609..1493)
                     /locus_tag="VF276_05735"
     CDS             complement(609..1493)
                     /locus_tag="VF276_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606402.1"
                     /translation="MKQCPNPDCLSYQQHRKFYDDDTICPRCGDVLVSTTAPAGAPVV
                     VDPYPAYAARPPAPPISTGLAMLGAVGTLLLVFLLIGLLLQLGMVHGRVAPGGAVSGT
                     LPVPTTVIFTPIAFTGAQATETARAIATAAAQPTPIGGLIGVTPTLPPLPGAVGGGGT
                     GGTSNPGGPSGGITNLTPITSINGVTAGLCRRIDAGALCDPVSVYGPNDTFYISVQAT
                     FGDGAARTIRVRLYGPPNGETPLLNQDQTNTPGRTGRFYVGFAFSQSVPWAPGIYRAD
                     VFVNNDTVRSTYITWTVQ"
     gene            1583..2506
                     /locus_tag="VF276_05740"
     CDS             1583..2506
                     /locus_tag="VF276_05740"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HEX6606403.1"
                     /translation="MRADRLRFGYTDSIHLLPLIHPLRAGWVYPTGLQLELVPLAPPA
                     LEQALGQGDLDGGLAGPLTYARLRGGLEAMPGIGLASTGPNSLAVLQSRTRPDQLDGQ
                     TVAVTPSAQETTAAALLQVLAGPFFGVNLAPAPESTVAPGGDTPPPRLLSGDAAVQAR
                     LPWLLYEARFSPSALAAEERALAKGRPLPAPTNPAPAPDPETAGYAEDLGAAWWVFSG
                     TPMVWALGVLRGEWLQQPEAVVAVVRAFQQSRVAAREQAATVLAAAAAQAQVPESAVQ
                     ALFDRQTVEIGAAEQGGLAAFYRYTGKLRLT"
     gene            complement(2514..3089)
                     /locus_tag="VF276_05745"
     CDS             complement(2514..3089)
                     /locus_tag="VF276_05745"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX6606404.1"
                     /translation="MKRPVPRHLIHRVLGPGPVVLVTASVKGHVDVTTVGWAMPLSGD
                     PPLVVIAVEPVRHLHDLISRSDEFTINVPHAGLLREVQHCGSVSGADHDKFAETGLHA
                     VSARQVESPWIEECVAHLECGVVGASEQGDHTLFVAEVLAAWAEETAFGEHWLPDQES
                     GRLLHHLGGRLYTSGERAIDAGAPPPPREEE"
     gene            complement(3427..4188)
                     /locus_tag="VF276_05750"
     CDS             complement(3427..4188)
                     /locus_tag="VF276_05750"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6606405.1"
                     /translation="MPQVQVNGANIYYEEAGTGTPLLLLHGLLETGGSHAHLMQTLAR
                     HYRVIAPDLRGYGRSGPEPRTFPPDVYERDTADMAALLDHLGIAQAVVMGVADGAEIA
                     LLLPIRHPELVRAVVAVSVTGALHPSLLDVLPTLDTWDTAPAPDDTSPRADAIREYGL
                     DGARAIWRGWAGAVRDRIAAGGNISMAEAGRITCPVLIINGADDTLNTPAMSHALAQA
                     IPNAELHLVADVDQQIYNKRRQWLADFVLTWLAAH"
     gene            4383..6242
                     /locus_tag="VF276_05755"
     CDS             4383..6242
                     /locus_tag="VF276_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606406.1"
                     /translation="MRCPFCGAANRADVTFCYACGESLDPEIAPAVPAPPPPALPRWR
                     RLPWELLLGAGILLVILAVAVVAGWQETIRTDQARHYRAGQQALAAGHLDEALPAFQR
                     AGSYRDAAGLSARLAPQVAQLEAGYQAGVAAAASGAWWDAARALSAAANIQASYRDVA
                     TRLQEARDHAGPIFYRMAGDNGEVALWWANADGTAPRRLPFSTPTSELLAVSPDHHWA
                     VYSHGPAAQAGADPQGPFLLDLQQVSVTSLAQRYRELQGAVRVRFRDDSQGFWWGYDN
                     QAFYYDLRAHTGLPLSELPAAIDPGHATLLLNRTLVGDSNVRGRLLLSDPLGRQRIPL
                     VEEPGEIRDARFSDDGRYLLYTVNTMPVKDAAGRRIVTATLVMQDLDAGDAPVRTELD
                     HVRASIDDQPIGWLRMDFIPNTSTVVILRGQGEHWTLSAQPANGAVHKLAEGAALGRQ
                     DPDLLAVLAVPNSSQLAASLTVANGRRTDLTLLSLDGGRTVIAPSTLSGRWMGFSPDG
                     HMALISQRDPKAATVARQVRLAPLDHSTAPATLPFALGPSGAGRAGDPVFTGDGRRLF
                     VVSTFDGHPGLYAVRPDGSDPQLVIAGATAFWTGQVMAHPLLTDHTNSMFLDP"
     gene            6329..>7619
                     /locus_tag="VF276_05760"
     CDS             6329..>7619
                     /locus_tag="VF276_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606407.1"
                     /translation="MRCPYCGTVNQQYATFCLGCGETLDAPRLADAATAESREASTGP
                     GTAPSPAPQQGSIRRLRLPRRIEGWIGIVCAVILLVAAIGDAWAQQSHIDNYRAGRAA
                     VAARDWDAATLEFARAGDYRDAAKQQRTAAKQVAERDHLYTTGAAAAEKGDWATARSA
                     FEGAARIQPAYADLKTRLAEARHEAGNRAAAGLVYRAQSGPGAGLYLQGPLDRPVAAL
                     PGSDAFSQVLAFSPDGRYVVYDGPGTGRQRSLSLAELAPGDGVVRTHRGLPASLSSSG
                     WGTFCPGGFWWFSSAAPTLSYYDLRTGQAGAVPGPAGSRLLHADSTHGALLFAAPATV
                     DGQPGTAVVRTAADGRQPRTILTSRGQVLSAELSPDGNWMLYQREEPGSMTIYGHGGE
                     DADSIGVWWDHERAGASTTLLMLTWLKPATQPWPHGPG"
BASE COUNT         1152 a         2731 c         2619 g         1117 t
ORIGIN      
        1 cgaagcgtcc cgccggggtg cagggctggt ttgtgcggta catcggcaca ttcgagcaaa
       61 gttcgatgtg gcgcacgggc acgcccgccg ccagcagggc gctctcgaag gtgaaactgc
      121 aaccgatcag gaaagccacc agatcgtcgc gccacaggtc gcggatatcg gtgcgctcgt
      181 ccacctgcac gccgtcgcgg tagacgcggt agcgcggcag gtcggtgcgc aggtcggcat
      241 ccggcgccag gagatgcggc accgggtcgc cgggcgcggt catttcgagc agcgggcagg
      301 gacgcgggtt gagttcgcag aattcgcgga aagcggcggc ctcggcggcg ggcaagataa
      361 ccaggttggc ctgcgcggag tcgggcgcct ggcccgccgt ggggccggta aacgcgccgg
      421 cccggatggc ggcgcgcagg ccggcggcgt cgtaggtggc ggcccgcggg gacggtgttt
      481 cgagggtcat atgtcggcat cccttcgcga cggaccgcac cggcaagccc ccaacccgga
      541 tctggcctct atcgccggcg ggccggtttg aaaccggtcc ctacagcggc tggatcctgg
      601 ggtcgcgcct attgcacggt ccaggtgatg taggtcgagc gcactgtgtc gttgttcaca
      661 aacacgtcgg cgcgatagat gcccggcgcc cagggcacgc tttgcgagaa ggcgaacccg
      721 acgtagaagc ggcccgtgcg gccgggcgtg ttggtctggt cctggttgag cagcggcgtc
      781 tcgccgttgg gcgggccgta gaggcggaca cggatcgtgc gcgccgcgcc gtcgccgaag
      841 gtcgcctgga ccgaaatata gaacgtatcg ttggggccgt agacactgac agggtcgcag
      901 agcgcgcccg cgtcgatgcg ccggcacagc cccgcggtca cgccgttgat cgacgtaatc
      961 ggggtcaggt tggtgatccc gccactcggg ccgccggggt tgctcgtccc gccggtgccg
     1021 cccccgccca ccgcgcccgg caagggcggc agcgtgggcg tgaccccgat cagcccgccg
     1081 atgggcgtgg gctgggccgc cgccgtggcg atggcccgcg ccgtctccgt cgcctgcgcc
     1141 ccggtaaacg cgatcggggt gaagatgacg gtcgtgggca cgggcagggt gccgctcacg
     1201 gcaccgcctg gggcaacccg gccatgaacc atacccaact gcagcagcag cccgatcagc
     1261 aggaacacca gcagcagggt gccgacggcg cccagcatcg ccaggccggt ggagatcggc
     1321 ggcgcgggcg ggcgggcggc ataggccggg tagggatcga ccacgacggg cgccccggcg
     1381 ggcgcggtcg tgctcaccag cacatcaccg cagcgcgggc agatcgtgtc gtcgtcgtag
     1441 aacttgcggt gctgctggta ggaaagacaa tccgggtttg ggcactgttt catgtccggc
     1501 tccctgccgg cgcacgcctt gccgcgccgc cgggcatatc gggtataatg aaacggaagt
     1561 ctacgtcact gaaaggtagc ccatgcgagc cgaccgcctc cgtttcggat acacggatag
     1621 tatacacctg ctgcctctca tccacccctt gcgcgccggg tgggtctatc ccaccgggct
     1681 gcaactggaa ctggtgcccc tggccccacc ggccctagag caggcgctgg gccagggcga
     1741 cctcgacggc gggctcgccg gcccgctcac ctatgcgcgc ttgcgcggcg gcctcgaagc
     1801 catgccgggc atcggcctgg cgagcaccgg ccccaatagc ctggccgtgc tgcaaagccg
     1861 cacccggccc gaccaactcg acggccagac cgtcgccgtc accccgtccg cgcaagagac
     1921 gacggcggca gcgctgttgc aggtgctggc cggcccgttt ttcggcgtga acctggcgcc
     1981 ggctcccgaa tccaccgtag caccgggcgg cgatacgccc ccaccccgcc tgctgagcgg
     2041 cgacgcggcg gtgcaggcgc ggctgccctg gctgctctac gaagcgcgtt tcagccccag
     2101 cgcactcgcc gccgaggaac gcgccctggc gaagggccgg cccctgcctg ctcccaccaa
     2161 cccggcgccc gcccccgacc ccgagaccgc cggctatgcc gaagacctgg gcgccgcctg
     2221 gtgggtcttc agcggcacgc cgatggtctg ggcgctgggc gtgctgcgcg gcgagtggct
     2281 gcaacagccc gaagccgtgg tcgccgtggt gcgcgccttc cagcagagcc gggtggccgc
     2341 ccgcgaacag gccgccacgg tgctggccgc agctgccgcc caggcccagg tgcccgaatc
     2401 cgccgtgcag gccctgttcg accggcagac cgtcgagatc ggggcggcgg aacagggcgg
     2461 gctggccgcc ttctaccgct acaccggcaa gctgcgcctc acctgagcgc cggctactcc
     2521 tcctcgcgcg gcgggggcgg ggcgcccgcg tcgatggcac gctcgccgct ggtgtagagt
     2581 cgcccgccca ggtggtgcaa cagccgcccg ctctcctgat ccggcagcca gtgctcgccg
     2641 aaagccgtct cctcggccca ggcggccagc acctcggcca caaacagcgt gtggtcgccc
     2701 tgctcgctgg cgcccaccac cccgcactcc aggtgggcca cgcactcctc gatccagggc
     2761 gattcgacct ggcgggccga aacggcatgg agcccggtct ccgcgaactt atcgtgatcc
     2821 gcgcccgaca cgctgccgca atgctgcacc tcgcgcagca ggcccgcatg gggcacgttg
     2881 atcgtgaact cgtcactgcg gctgatcagg tcgtgcaaat ggcgcaccgg ctcgacggca
     2941 atcaccacca gcggcgggtc gccgctcagc ggcatagccc agccaaccgt ggtcacatcg
     3001 acatggccct tcaccgacgc ggtcacgagc accaccggcc ccggccccag cacgcggtgg
     3061 atgagatggc gcggcaccgg tcgtttcatg aatcccgcct cccacggcgc ttccctgatc
     3121 gcggcctagc gctggtcgta gcaggaagcg cgccattgtt atgttaagcc aattatatgc
     3181 gggattcgcg ggttgcgcaa ggggcagcac cggatcagac gggtgcaatc ataagaggat
     3241 caggcgcggg gacacccggt gagccaccat ccgcccggcg ggaccagaag ggggccggct
     3301 agcgaccgac caccgtgaat tgcgcgctgt aggggccggt ttcaaaccgg catgccgccc
     3361 aagtagggct gtaggggccg gtttcaaacc ggcctggccg cccattggaa tcggtgtaac
     3421 ggtctactaa tgggcggcga gccaggtgag gacgaagtcg gccagccact ggcgacgctt
     3481 gttgtagatc tgctggtcca cgtcggcgac caggtggagt tcggcgttgg ggatggcctg
     3541 ggccagcgcg tggctcatcg ccggggtgtt gagcgtgtca tcggccccgt tgatgatcag
     3601 caccggacag gtgatgcggc ccgcttcggc catgctgatg ttgccccccg cggcaatccg
     3661 gtcgcggacg gcgccggccc agccgcgcca gatggcccgc gcgccatcca ggccatattc
     3721 gcggatcgcg tccgcgcgcg ggctggtgtc gtcgggggcg ggcgcggtat cccaggtgtc
     3781 gagagtgggt agcacgtcga gcaagctggg atgcagagcg ccggtcacgc tcaccgccac
     3841 cacggcgcgc accagttccg gatggcggat cggcagcaac agcgcgatct cggcgccgtc
     3901 ggccacgccc atgaccacgg cctgggcaat tcccagatga tcgagcaagg ccgccatgtc
     3961 ggcggtatcg cgctcataaa cgtccggcgg aaaagtgcgc ggctccggcc ccgaccggcc
     4021 atagccgcgc aggtcgggcg cgatcacccg gtagtggcgg gccaaggtct gcatcaggtg
     4081 ggcatggctg ccgccggttt cgagcaaccc gtggagcagc agcagcggcg tacccgtgcc
     4141 cgcttcttca taatagatgt tcgccccgtt gacctgcacc tgcggcataa ccccctcctc
     4201 ctcgcaccgt ctctaccgcg ctatcctacc acaaggtgcc acgtctcgca ataggacagg
     4261 tgcacgagcg cgcgccataa gcataaaatc ggtgaaccgc aaatcgggcg caggttgtag
     4321 ttttcataga ctttgggtgt ataatgagac cagggtccgt tagccatgtc aggagaccac
     4381 gcatgcgctg tccattctgc ggagcagcca atcgcgccga cgttaccttt tgctacgcct
     4441 gcggggaatc gctggatccg gagatcgcgc ccgccgtgcc ggcgccgcca ccccccgccc
     4501 tcccgcgctg gcgccgcctg ccctgggaac tgctgctggg cgccggcatc ctgctggtta
     4561 tcctcgcggt cgccgtggtc gcgggctggc aggagacgat ccgcaccgac caggcgcggc
     4621 actaccgcgc gggccagcag gcgctggccg ccgggcacct cgacgaggcc ttgcccgcct
     4681 tccagcgcgc cgggtcgtac cgggacgccg ccgggctgag cgcccgcctg gcgccccagg
     4741 tggcccaact ggaagcgggc tatcaggcgg gcgtggcggc ggcggcatcc ggggcctggt
     4801 gggacgcggc ccgtgcgctg agcgccgccg ccaacatcca ggcctcgtat cgcgatgtgg
     4861 cgacccgctt gcaggaagcc cgcgaccatg ccggcccgat cttctaccgc atggccggcg
     4921 ataatggcga agtggccctc tggtgggcca atgccgacgg caccgccccg cggcgcctgc
     4981 ccttcagcac tcccacctcc gaactgctcg ccgtctcgcc cgaccatcac tgggccgtct
     5041 acagccatgg tcccgccgcc caggcgggcg cggacccaca aggccccttc ctgctcgact
     5101 tgcagcaggt gagcgtcacc tcgctggccc agcgctaccg cgagctgcag ggggccgtgc
     5161 gcgtgcgttt ccgcgacgac agccagggct tctggtgggg ctacgacaac caggcgtttt
     5221 actatgacct gcgcgcccat accggcctgc cattgagcga gctgcccgct gcaatcgatc
     5281 ctggtcatgc caccctgttg ctgaaccgca ccctggtggg cgacagcaac gtgcggggcc
     5341 ggctgctgtt gagcgatccg ctgggccgcc agcgcatccc gctggtggaa gagccgggcg
     5401 aaatccgcga cgcccggttc agcgacgatg ggcgctactt gctctatacc gtgaacacga
     5461 tgccggtgaa ggacgctgcg ggccggcgca tcgtcacggc cacgctggtc atgcaagacc
     5521 tggacgccgg ggatgccccc gtacgcacgg aactcgacca tgtgcgtgcc tcaatcgacg
     5581 atcagcccat cggctggctg cgcatggatt ttatcccgaa tacgtcgacg gtcgtcatcc
     5641 tgcgtggcca gggcgagcac tggacgctca gcgcccagcc cgcgaacggg gccgtccata
     5701 aactggccga gggcgccgcc ctgggccggc aagatcccga tctgctcgcc gtgctggccg
     5761 tgccgaacag cagccaacta gccgccagcc tgactgtcgc caacggccgt cgcaccgatc
     5821 tcacgctgct gagcctcgac ggcgggcgga cggtcatcgc gccgagcacg ctgtcgggcc
     5881 gctggatggg cttctccccc gacggccaca tggcgctgat ctcgcagcgc gatccgaagg
     5941 ccgccactgt tgcccgccag gtgcggttgg cgcccctgga ccatagcacc gctccggcca
     6001 cgctgccgtt tgcgctcggt ccgtccggcg ccggacgggc cggcgacccg gtcttcaccg
     6061 gcgacgggcg gcgcctgttc gtggtcagta ccttcgacgg ccatccgggc ctctacgcgg
     6121 tgcgtccgga cggcagcgat ccgcagttgg tgatcgcggg cgccaccgcg ttctggaccg
     6181 gccaggtcat ggcgcacccc ttgcttacag atcacaccaa cagtatgttc ctggacccgt
     6241 gaatctccgc cccggcgcta cacggggcgg gattcgatat ggatacgtga tcccggcgac
     6301 gccgggtgaa gcctgacggc gggctgccat gcgatgccct tattgcggca cggttaacca
     6361 gcagtacgcg actttctgcc tgggctgcgg agagacgctg gacgccccgc gccttgctga
     6421 tgctgcgaca gcagagtccc gtgaagcgag cacaggaccg ggcactgcgc caagccccgc
     6481 gccacagcag gggagcatcc gccggctgcg cctgccgcgt cgcatcgagg gctggatcgg
     6541 tatcgtctgc gccgtgattt tgctcgtggc cgcgatcggc gatgcctggg cgcagcagag
     6601 ccacatcgac aactatcgcg ccgggcgcgc cgccgtcgcc gcccgcgact gggacgccgc
     6661 cacgcttgaa ttcgcccgcg ctggcgacta ccgcgatgcc gccaaacagc aacgcaccgc
     6721 cgccaagcag gtggccgagc gcgaccacct ctacaccacc ggcgccgccg ccgcggagaa
     6781 gggcgattgg gccacggccc gcagcgcgtt cgagggcgcc gcacgcatcc agccggccta
     6841 cgccgacctc aagacgcgcc tggccgaggc ccggcacgaa gccggcaatc gcgccgccgc
     6901 ggggctggtc taccgggcgc aaagcggtcc cggtgccgga ctctatctcc aggggccact
     6961 ggaccggccg gttgccgcct tgccgggcag cgacgccttc agccaggtgc tggccttctc
     7021 gcccgacggg cgctacgtcg tctacgatgg gccgggcacc gggcggcagc gcagcctgtc
     7081 gctggccgaa ctcgcgcccg gcgacggcgt cgttcgcacg caccgagggc tgcccgcgag
     7141 tctctcttca agcggctggg gcacgttctg tcccggcggt ttctggtggt tcagctcggc
     7201 ggccccgacg ctcagctatt acgacctgcg cacgggccag gccggcgcgg tgcccggtcc
     7261 cgcgggcagc cggctgctgc acgccgacag cacccacggc gccctgttgt tcgccgcccc
     7321 ggccacggtc gacggccagc cgggcaccgc cgtcgtccgc accgccgccg atggccgcca
     7381 gccgcgtacc atcctgacca gccgcggcca ggtcctcagc gccgagttga gtcccgacgg
     7441 caactggatg ctctatcagc gcgaagaacc cggttccatg acgatctacg gccacggcgg
     7501 cgaggatgcc gactccatcg gcgtctggtg ggaccatgag cgcgccggcg ccagcaccac
     7561 gctgctcatg ctgacctggc tcaagcccgc gacccagccc tggccgcacg gaccgggcg
//
LOCUS       DASUYC010000349         2732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1959999, whole genome shotgun sequence.
ACCESSION   DASUYC010000349 DASUYC010000000
VERSION     DASUYC010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1959999"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1526)
                     /locus_tag="VF276_05765"
     CDS             complement(<1..1526)
                     /locus_tag="VF276_05765"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEX6606408.1"
                     /translation="MQPQQKAAGVRIAALALIIAPLLAGQAPARAADPAGFADPAFER
                     VWQRTDAPVAAEKANRSWYWGPTPGASVLEPMREAPGGQRRVQYFDKTRMEINDPAGD
                     KTSPWYVTNGLLAVELMTGRMQVGVNSFEQRCPANIPLASDTTDSNAPTYATFGTLMA
                     QPKRDLTGQKALAVTAKTGVVSSDPARTTVPGITFVHYEPLTGRNIPAIFWDFLNTTG
                     PIVVNGQYQTGKVNDPWFFASGLPVTDPYWAKVKIAGQPTDVLIQAYERRVLTYIPAY
                     KAPFNVQMGNVGQHYYDWRYKGAGCAATAPLVLPRYVVDAGDFDKVEAVAAVKAAGIP
                     MVRLPLWWAAVEAQPSDPSGYKWSYYDTVMRWYSQAGIQVMPTINSCPAWACTNEAGP
                     VDKVPVDRLVTFWKAVADRYSRPPYNVHFWELFNEPDSTYNPDHPTGFGVNAAAYVAL
                     LRAGVPAVRAVDPQARFVLGGLGYEWFMDETPPGPFNRNFLRDFAQAGGGDYLDYVNF
                     "
     gene            complement(1702..>2732)
                     /locus_tag="VF276_05770"
     CDS             complement(1702..>2732)
                     /locus_tag="VF276_05770"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEX6606409.1"
                     /translation="MQAAYVVEAYAKALSLGISTVAWFPLHDFTSEEYWYFQYHGLVR
                     ADYTPKPALTSYTTAARYLGTATALRALRPDEFTGPNPAEGYSFAVADGTGLLVAWST
                     GQSNLTLPASVVTDARDMYDNLRPYTVSGGQATIALDPAPVYIKLRTPARFGDVSFND
                     YFYTPVEYLVTHGAVSGYSDGTYRPGSNATRGQISKMVSLAMGWPVDNNAPQVFADVL
                     PGSTFYGVIGAAYAHGIISGYDCGQAAGEPCDAQHRPYFRPANNVTRGQLSKMLVRAK
                     GWAEVPPTSGIQSFTDVPPTYPFYGVIEAAVAHGVVSGYSDRTFRPGNQATRGQLAKM
                     IYVAVTQP"
BASE COUNT          403 a          881 c          984 g          464 t
ORIGIN      
        1 tggaagttga cataatcgag atagtcgccg ccgcctgcct gggcgaagtc gcgcaggaag
       61 ttgcggttga acgggccggg cggcgtctcg tccatgaacc actcgtagcc cagcccgccc
      121 agcacgaacc gggcttgcgg atcgacggcg cgcacggcgg gcaccccggc gcgcagcagc
      181 gccacatagg ccgccgcatt caccccgaag ccggtcggat ggtccgggtt atacgtggag
      241 tccggttcgt tgaacaactc ccagaagtgg acattatagg gcgggcggct ataacgatcc
      301 gccaccgcct tccagaatgt gaccaggcga tccaccggca ccttatccac cggcccggcc
      361 tcgttcgtgc aggcccaggc ggggcaggag ttaatggtcg gcatgacctg gatgccggcc
      421 tgggaatacc agcgcatcac cgtgtcgtag taggaccact tgtagccgct gggatcgctc
      481 ggctgcgcct ccacggcggc ccaccatagc ggcaggcgca ccatcgggat cccggcggcc
      541 ttcactgcgg ccaccgcttc caccttgtcg aagtctccgg cgtccacgac gtagcggggc
      601 aggaccagcg gcgcggtcgc cgcgcagccc gcgcccttgt agcgccaatc gtagtagtgc
      661 tggcccacgt tgcccatctg cacgttgaac ggcgctttgt acgcggggat gtaggtcagc
      721 acgcgccgct catacgcctg gatgagcacg tcggtaggct gcccggcgat tttgactttc
      781 gcccagtagg ggtcggtcac gggcagcccg ctggcaaaga accatgggtc gttgaccttg
      841 ccggtctggt attgcccgtt gaccacaatc gggccggtgg tgttcaggaa atcccaaaag
      901 atagcaggaa tgttgcgccc ggtcagcggc tcgtagtgga cgaaggtgat gcccggcacg
      961 gtggtgcgcg ccgggtcgct gctgacaacg cccgtcttgg cggtgacggc cagcgccttc
     1021 tggccggtca ggtcgcgctt aggctgggcc atcagcgtgc cgaaggtagc ataggtcggg
     1081 gcgttggagt cggtggtgtc ggaggccagc gggatgttgg ccgggcagcg ctgctcgaag
     1141 ctgttcacgc cgacctgcat gcgcccggtc atcaactcga cggccagcaa cccgttggtg
     1201 acataccagg ggctggtctt atcgcccgcc gggtcgttga tctccatgcg cgtcttgtcg
     1261 aaatactgga cgcgccgctg gccgccgggc gcctcgcgca tcggctccag cacgctggcg
     1321 ccgggggtgg ggccccagta ccacgaacgg ttggctttct ccgcggcgac aggcgcgtcg
     1381 gtgcgctgcc agacccgctc gaaggccggg tcggcgaagc ccgccggatc ggcggcgcgg
     1441 gcgggcgcct gtccggccag cagcggcgcg atgatcaggg ccagcgcggc gatccgcacc
     1501 ccggcggctt tctgctgcgg ttgcatgggt tcggggtcct ttcgcatggt gacagggatg
     1561 ccgggcgcgc cgtgcgccgg gccggactgg caaagacacg gcgcgggcgc cggcctcccg
     1621 gcaggcctcc cgcgccgctg gtctgcgtga ttctgccctc accctcagaa cgaacgggcg
     1681 gggcggcggt tgccggtgtg cctacggctg cgtcaccgcg acgtagatca ttttcgccaa
     1741 ctggccgcgc gtggcctggt tgccggggcg gaacgtgcgg tcgctgtagc cgctgaccac
     1801 gccgtgggcg acggcggcct cgatgacgcc gtagaacgga taggtgggcg gcacgtcggt
     1861 gaagctctgg atcccgctgg tgggtggcac ctcggcccag cccttggcgc gcaccagcat
     1921 cttgctcaac tggccgcgcg tcacgttgtt ggccgggcgg aagtagggcc ggtgttgcgc
     1981 gtcgcacggt tcgcccgccg cctgcccgca gtcatagccg ctgatgatgc cgtgggcgta
     2041 ggccgcgccg atcacgccgt agaacgtcga gccgggcagc acgtcggcga acacctgcgg
     2101 cgcgttgttg tccaccggcc agcccatcgc cagcgagacc atcttgctga tctggccgcg
     2161 ggtggcgttg ctgccggggc ggtaggtgcc gtcgctgtag ccgctgacgg ctccgtgcgt
     2221 gaccaggtat tccacggggg tatagaagta atcgttgaag ctcacgtcgc cgaagcgggc
     2281 aggggtgcgc agcttgatat acacgggcgc ggggtccagg gcgatggtcg cctgcccacc
     2341 gctgaccgtg taagggcgca ggttgtcgta catatcgcgc gcgtcggtca cgacgctcgc
     2401 gggcagggtc aggttgctct gcccggtgct ccaggcgacg agcaggccgg tgccgtcggc
     2461 caccgcgaag gagtagccct cggcggggtt ggggccggtg aactcgtcgg ggcgcagcgc
     2521 gcgtagcgcc gtcgccgtgc ccaggtagcg cgcggctgtg gtatagctgg tcagcgccgg
     2581 tttgggcgtg taatcggccc gcaccagccc atggtactgg aagtaccagt attcctcgct
     2641 ggtaaagtcg tgcagcggga accaggccac cgtggagatg cccaggctca gcgctttggc
     2701 gtaggcttcg acgacatagg ccgcctgcat ct
//
LOCUS       DASUYC010000350         2185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_561128,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000350 DASUYC010000000
VERSION     DASUYC010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561128"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2160)
                     /locus_tag="VF276_05775"
     CDS             complement(<1..2160)
                     /locus_tag="VF276_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606410.1"
                     /translation="MSLPRTHWSFVRALLLLLLVLAPVASLRPRPSEGASGFVQVAPG
                     NTRFLRPDGRPFFVWAVNYVGAPDRAWRMWEDGQFDPAVIDADFTRAGALGLNTVRLF
                     VQPPLRADVQRNNFRKLDLVVDLARRHGLFLILTFTDYVEPDLQQVADIESRIAAHFR
                     DEATILAYDLKNEPQFDLIALSVYPGMPPPLQTDRLITLYGERISSAAIPAYRQTPEG
                     RALVPARLTDAQAYIYVNSYRLYREFLDAGASWVATHPDKTTLDYMDSPDAMNWRPFF
                     GVLDDTVRRWIAPQRDAIRAVDPNHLLTLAYSNIVLAKLSANRLLDFQSPHRFVATGM
                     ANLQRTLQVLTNLRTTFAGQPFMLEEFGYSNEEGLQRGASPVDQLVTANLETAVWLYL
                     YSQGYAGGGKWMLNNFPGGENASQNAYGLYDDNLQPKIAAYALSALMPYMASDPAPGT
                     FSDLNADQAGGVRFTYSAPNARFLGGTTSPAPDVVQYTANGSLPLLVFLTWQREVARQ
                     VDVWTSGPGSVSVNLPATLGRIGPDVALGVRVRQADGTWTPVPFTRNGDQVQIAAAGL
                     RTYRITAPSSALDPAPPSNQPGTIYFTQTHHNLGGGFLEYWRAHGGLPIFGYPLTEEF
                     NEGGYTVQYFERNRFEYHPEYAGTPYSVLLGRLGATVTAGRVFPTIPPFPNRPGHVYF
                     PPTQHSLNSGFLTYWQAHGGLAQFGYPISEEIAEVSPT"
BASE COUNT          352 a          667 c          833 g          333 t
ORIGIN      
        1 ggtggggctg acctcggcga tctcctcgga gatgggatag ccgaactggg ccaggccgcc
       61 gtgcgcctgc cagtaggtca ggaagcccga attgagcgag tgctgggtgg gcgggaaata
      121 gacgtggccg ggccggttgg ggaagggcgg gatggtcggg aagacgcgcc ccgccgtgac
      181 ggtggcgccc aggcggccca gcagcaccga gtagggcgtg cccgcgtatt cggggtggta
      241 ctcgaagcgg ttgcgctcga agtactgcac cgtgtagccg ccctcgttga attcctcggt
      301 cagcgggtag ccgaagatgg gcaggccgcc atgcgcccgc cagtactcca ggaagccgcc
      361 gcccaggttg tggtgcgtct gcgtgaagta gatcgtgccc ggctggttcg acggcggcgc
      421 cgggtcgagc gccgaactgg gcgcggtgat gcgataggtg cgcagcccgg cggcggcgat
      481 ctgcacctgg tcgccgttgc gggtgaaggg cacgggcgtc caggtgccgt cggcctggcg
      541 cacgcgcacg cccagcgcca cgtccggccc gatgcggccc agcgtcgcgg gcaggttcac
      601 gctcaccgac cccggcccgc tggtccagac atcgacctgc cgggccacct cgcgctgcca
      661 ggtgaggaac accagcagcg gcaggctgcc gttggccgta tactgcacca cgtcgggagc
      721 gggcgaggtg gtgccgccca ggaagcgggc gttgggcgcg ctgtaggtga agcgcacacc
      781 cccggcctgg tcggcgttca ggtcgctgaa ggtgcccggc gccgggtcgg aggccatgta
      841 gggcatcagc gccgagaggg cgtaggccgc gatcttgggc tgcaagttat cgtcatacag
      901 cccgtaggcg ttctgggacg cgttttcgcc gccggggaag ttgttgagca tccacttgcc
      961 gccgcccgcg tagccctgac tgtagaggta gagccagacc gccgtttcca ggttcgccgt
     1021 caccaactgg tccaccggcg aggcgccgcg ctgcaagccc tcttcgttcg agtagccgaa
     1081 ctcttcgagc atgaacggct gcccggcaaa cgtcgtgcgc aggttggtca gcacctgcaa
     1141 cgtgcgctgg aggttcgcca tgccggtggc gacgaagcgg tgcggcgact ggaagtcgag
     1201 caggcggttg gcgctgagtt tcgccagcac gatgttgctg taggccaggg tcagcaggtg
     1261 gttggggtcc acggctcgaa tcgcgtcgcg ctggggcgcg atccagcggc gcaccgtgtc
     1321 gtcgagcacg ccgaagaacg ggcgccagtt catcgcgtcg ggcgagtcca tgtagtcgag
     1381 cgtcgtctta tcgggatgcg tcgccaccca actggccccg gcgtcgagga actcgcggta
     1441 caggcgatag ctgttgacat agatgtaagc ctgggcgtcg gtgaggcggg cgggcaccag
     1501 cgcgcgcccc tccggcgttt gccggtaggc cgggatggcg gcagacgaaa tgcgctcgcc
     1561 gtagagggtg atcaggcggt cggtttgcag gggcggcggc atgccgggat agaccgacag
     1621 ggcgatcagg tcaaactgcg gctcgttttt caggtcatag gccagaatgg tcgcctcgtc
     1681 gcggaagtgc gcggcgatgc gcgactcgat gtcggccacc tgttgcaggt cgggttcgac
     1741 gtagtcggtg aaagtgagga tcaggaacag gccgtgccgc cgggccaggt ctaccaccag
     1801 gtcgagctta cggaagttgt tgcgctgcac gtcggcgcgc agtggcggct ggacgaagag
     1861 gcgcaccgtg ttcagcccga gggccccggc acgggtaaag tcggcgtcga tcaccgccgg
     1921 gtcgaactga ccgtcttccc acatacgcca ggcgcggtcg ggcgcgccga cgtagttgac
     1981 ggcccagacg aagaaggggc ggccgtcggg gcgcaggaaa cgggtgttac cgggcgctac
     2041 ctggacgaac ccggacgcgc cttcgctggg ccgggggcgc agcgaggcca ccggcgccag
     2101 caccagcaac agcagcagca gcgcgcggac gaagctccaa tgtgtgcgag gcaaggacat
     2161 agcgaggacc caggggcgcg actgc
//
LOCUS       DASUYC010000351         2083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1688903, whole genome shotgun sequence.
ACCESSION   DASUYC010000351 DASUYC010000000
VERSION     DASUYC010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1688903"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1139
                     /locus_tag="VF276_05780"
     CDS             <1..1139
                     /locus_tag="VF276_05780"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX6606411.1"
                     /translation="LGIAATFWVLGPIVRRITGITGDLQRMAEGDLTLPGRLAHMDLD
                     APTGANDEVGSLERAYIQTLDRLRVPLQRIQEDAARISASSTEISSAATHQAAGSSQQ
                     ATAITEVTVTVEQLNQTAVQIADAAASVASAAEQALVSASRGQEAVRDSIIGMAMIRS
                     RVNDITARILALSAQSQRISDIIDVIDDMAARTHILALNAAVESAGAGGEVGERFGVV
                     ASEVKKLAQRSAGATREVRAVIAQVQAATNAAVMATEDGLKETEKGVQMAHQSGDANE
                     DIIQMVERTAQLANAISLATQQQRTASEQVVGTMREIAQVTRQAATSSEQASRAASEL
                     SDIAQELRTVSAGFRVERETGSPPPADPDPRPAPRLATGESLAT"
     gene            1184..>2083
                     /locus_tag="VF276_05785"
     CDS             1184..>2083
                     /locus_tag="VF276_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606412.1"
                     /translation="MTMQPEQMEQNTGVAPSIPAQTHTSSRFVRSGLFWRMMSGALIA
                     FVMALVTAGLSLWVISYLVQETDQMRAAATSAGDVQRLSTALESSYATLDLMSDAGYQ
                     QRFYQVQHDNAMAARTALGRTQVPAAYSTRRNDILTRFDSLEPALQELNQLSATDRAQ
                     AVARWQNTYHGQLFALNQDVEGLATDLNKAAGEAVVDMSSGQEMARIAIPVISVAGLL
                     LGLLLALLLLRGTVHRIRGVSSDLARLANGDLTPPLRLRHAAPRQQTGFFSDEVLALE
                     QDYLRTVERLRAPLQRIQEDAARI"
BASE COUNT          367 a          743 c          662 g          311 t
ORIGIN      
        1 ttctagggat tgcggcgact ttttgggtgc ttggtcccat cgtgcggcgc attaccggga
       61 ttacgggcga cttgcagcgc atggccgagg gcgacctcac actgccgggg cgcctggcgc
      121 acatggacct cgatgccccg accggcgcca acgatgaggt tggctccctc gaacgcgctt
      181 atattcaaac gctggatcgc ctgcgcgtgc ccttgcagcg catccaggag gacgccgccc
      241 gtatcagcgc ctcctccacc gagatctcct ccgccgccac ccatcaggcc gccggctcca
      301 gccagcaggc caccgccatc accgaagtca ccgtcaccgt cgaacaactc aaccagactg
      361 ccgtccagat cgccgatgcc gccgcctccg tcgcctccgc cgccgaacag gccctcgtct
      421 ccgcctcccg cggccaggaa gccgtccgcg actccatcat cggcatggcc atgatccgct
      481 cccgcgtcaa cgacatcacc gcccgcattc tcgccctctc ggcccagtcc cagcgcatca
      541 gcgatatcat cgatgttatc gacgacatgg ccgcgcgcac ccatatcctg gccctcaacg
      601 ccgccgtcga gagtgccggg gccggcggcg aggtcgggga gcgcttcggc gtggtcgcca
      661 gcgaggtcaa gaagttggcc cagcgctcgg cgggggccac gcgcgaggtc cgcgccgtca
      721 tcgcccaggt gcaggcggcg accaacgccg ccgtgatggc caccgaggac gggctcaagg
      781 agaccgagaa gggggtgcag atggcccacc agtcagggga tgccaacgag gacatcatcc
      841 agatggtgga gcgcacggcg cagttagcga acgcgatcag cctggcgacg cagcagcagc
      901 ggacggcgag cgagcaggtg gtgggcacga tgcgcgagat cgcgcaggtg acacggcagg
      961 cggcgaccag cagcgagcag gcctcgcggg cggcgagcga gttgagcgac atcgcccagg
     1021 aactgcgcac ggtttccgcc ggcttccgcg tcgaacgcga gacgggcagc ccgccgcccg
     1081 ccgatcccga tccccgtccg gccccgcgcc tggcgacggg ggaaagcctc gccacataaa
     1141 ttttgatccg gcaatctgaa acgatgatag gagtagtacg agcgtgacga tgcaacctga
     1201 gcagatggag cagaataccg gcgtggctcc gagtatccct gcccaaaccc acacgtccag
     1261 ccggttcgtc cgcagcggcc tgttctggcg catgatgagc ggcgcgctga tcgccttcgt
     1321 gatggcgctg gtgacggccg gtctctcgct gtgggtcatc agctaccttg tgcaggagac
     1381 cgaccagatg cgcgcggcgg ccaccagtgc cggcgacgtg caacgcctct ccacagcgct
     1441 cgaaagctcc tacgccacgc tcgacctgat gtcggatgcc ggctaccaac aacggtttta
     1501 ccaggtccag cacgacaacg ccatggcggc ccggaccgcc ctgggccgta cgcaggtccc
     1561 cgcggcgtac agcacccgcc gaaacgatat tctcacccgc ttcgactctc tcgaaccggc
     1621 acttcaggag ttgaatcagc tttcggcgac cgatcgagct caggcggtcg ctcgctggca
     1681 aaacacctat cacgggcaac tattcgcgct gaatcaagat gtcgagggcc tggccaccga
     1741 tctcaataag gcggccgggg aggccgtggt cgatatgagc agcggccagg aaatggcgcg
     1801 gatcgccatc ccggttatca gcgtcgcagg gctgttgctg ggcctcttgc tcgccctgct
     1861 gctgctgcgc ggcacggtgc accgcatccg cggcgtgagc tccgacctgg cccggctggc
     1921 gaacggcgat ctgaccccgc cgctgcgcct gcgccatgcc gccccccggc agcagaccgg
     1981 cttcttctcc gacgaagtgc tggccctgga gcaagattac ctgcggaccg tcgaacgcct
     2041 gcgggcgccg ctgcagcgca tccaggagga cgccgcccgc atc
//
LOCUS       DASUYC010000352         3530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1853007, whole genome shotgun sequence.
ACCESSION   DASUYC010000352 DASUYC010000000
VERSION     DASUYC010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1853007"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VF276_05790"
     CDS             complement(<1..591)
                     /locus_tag="VF276_05790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HEX6606413.1"
                     /translation="MSALDAPGHPSAPPRPRLIKCLVWDLDETLWAGVQAEGDQPEAR
                     PAALALMDTLSRRGIVQSVASRNDPAAGARLLALPALAGRFVAPQVGWEPKDVALRRI
                     AKELNIGLDSLAFVDDSPFERAAVAAVLPEVLVLAPADLPALAARPEFAGGPGTAEAA
                     ARTRLYQEDAARRAAERDFAGDRARFLESCTMGLEVR"
     gene            complement(579..947)
                     /gene="speD"
                     /locus_tag="VF276_05795"
     CDS             complement(579..947)
                     /gene="speD"
                     /locus_tag="VF276_05795"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:TIGR03330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HEX6606414.1"
                     /translation="MRNRHVLYDIWVDDAKPLERVGPLEDLLHRAALAGGATILHGYF
                     HPFAPCGVTGFLLLAESHLSVHTWPEEGFAAFDIFSCGDMDTDAIVDTIRATLRPARE
                     RLQLIHRGSGEGEGGPSCPR"
     gene            complement(940..1497)
                     /locus_tag="VF276_05800"
     CDS             complement(940..1497)
                     /locus_tag="VF276_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013016738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6606415.1"
                     /translation="MSKKRDPRPAAPAASAPPVPPMPEPVEVPAPEGPAVIRPAQEAD
                     LPALVAFEIAIARISFPRDAVTDPAVHEKKLRKALERGEPGMFVLVREGQVLGWLWAT
                     FNTNFLTNEHYATFRSLAVDDAAPDHAALAEALFNYGIAYAQEAGMLEITGKVHVNNT
                     PMRLLYKKAGFEAEHLTMKLPLTNA"
     gene            complement(1494..2705)
                     /locus_tag="VF276_05805"
     CDS             complement(1494..2705)
                     /locus_tag="VF276_05805"
                     /inference="COORDINATES: protein motif:HMM:NF013978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis-aminopropyl spermidine synthase family
                     protein"
                     /protein_id="HEX6606416.1"
                     /translation="MGDNKEPATGAANTLLSDLAARTRLREGAAGIEAVLRTIHRSGG
                     LTGKDLARQTRLPIPVVTAIRRELEKAGLVQPGPYIRLSPQGLVLVEGAWGWVGQGFL
                     CPLCQGRHVVAGSEWEGVLAQLEAWFAANPTVDVTLDQSHCTPETNLRRVLYMQTQGA
                     LAGKAVLLLGDDDSVSLAIGLVGQLLSPQGGGRLTRRLCVVDTDPRILAHLRACAEEA
                     GLEIETVEHNLRDPLPEDLLHAFDTVVTDPPYTAVGLDLFVSRAVSALVPEQGRQIFL
                     SFGHRPPAEQAALQGSLAAMGLAIAELIPDFNEYRGAGILAGVSQMIRLQTTPASRPK
                     ITGQFSGPLYTGEMNPTVRVYRCMGCGTKLRVGQAPDAAAPSIEQLKAAGCPRCGSVL
                     FLLESREAVDS"
     gene            complement(2913..>3530)
                     /locus_tag="VF276_05810"
     CDS             complement(2913..>3530)
                     /locus_tag="VF276_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606417.1"
                     /translation="LTTGDEDRFDDSILEELPDVYGMIGPAAIPALSALLADDSQGIY
                     GRMSALTALGKIAEQYPDARDAALAPIVRQLEAYATNDEDLNGFLISELMRLQAAETL
                     PLIEQVFKAGKVGPMMATWDDVQIEFGLKTPEEVGRDPDQPLADPLRVQRIQEMLGSA
                     PAPSIKPFRPSATARTGAGGASPGDKAKSKRHMGKESRKKNKKHK"
BASE COUNT          541 a         1264 c         1182 g          543 t
ORIGIN      
        1 gcgcacctcc aaccccatgg tgcacgactc caggaagcgg gcgcggtccc cggcgaaatc
       61 gcgctcggcg gcgcggcggg cggcgtcctc ctggtagagc cgcgtgcgcg ccgccgcctc
      121 ggcggtgccc ggcccgccgg caaactcagg ccgggcggcc agcgcgggca ggtccgccgg
      181 ggccagcacc agcacctcgg gcaacacggc ggccacggcg gcacgctcaa aggggctgtc
      241 gtccacgaag gccaggctgt ccaggccgat gttcaactct ttggcgatgc ggcgcagcgc
      301 cacatccttc ggctcccagc ccacctgtgg cgccacgaag cgcccggcca gcgcgggcaa
      361 ggccagcagc cgcgcgcccg ccgccgggtc gttgcggctg gcgacgctct gcacgatgcc
      421 ccgccgcgat agggtgtcca tcagcgccag cgccgccggg cgcgcctccg gctgatcgcc
      481 ctccgcctgc acccccgccc acaacgtctc gtccaggtcc cagaccaggc acttgatcag
      541 gcgcggcctc gggggggcgg aaggatgacc gggcgcgtct agcgcggaca actcgggcct
      601 ccctcgccct cgccgctgcc ccggtggatc agttggaggc gttcgcgggc cgggcgcaag
      661 gtcgcgcgga tggtatcgac aatcgcatcg gtgtccatgt cgccgcagga gaagatgtcg
      721 aaggcggcaa atccttcttc gggccaggta tgcacgctca ggtgggactc ggccagcagc
      781 agaaagccgg tgacgccgca cggagcaaag gggtggaaat agccgtgcag gatcgtcgcg
      841 cccccggcca gcgcggcgcg gtgcagcaga tcctccagcg gcccgacacg ctccaggggc
      901 ttcgcgtcgt ccacccagat atcataaagt acgtgacgat tacgcattgg ttaacggcaa
      961 cttcatagtt aagtgttcgg cttcaaagcc ggctttcttg tagagcaagc gcatgggcgt
     1021 gttgttcaca tgcaccttgc cggtgatctc cagcataccg gcctcttgag cataggcaat
     1081 gccatagtta aacagcgcct cggccagggc ggcgtggtcg ggcgccgcgt cgtccaccgc
     1141 caggctgcga aaggtggcgt agtgctcgtt ggtcaggaag ttggtgttga aggtggccca
     1201 gagccagccc agcacctgcc cctcgcggac cagcacaaac atgcccggct cgccgcgctc
     1261 cagcgccttg cgcagcttct tctcgtggac cgccgggtcg gtgacggcgt cgcgcgggaa
     1321 cgagatgcgc gcgatggcga tctcgaaggc gaccagcgcg ggcaggtcgg cctcctgggc
     1381 cgggcggatg accgccggtc cctccggcgc gggcacctcg accggctccg gcatgggcgg
     1441 cacaggcggc gcggaggcag cgggcgcggc ggggcgcggg tcgcgcttct tgctcacgag
     1501 tccaccgcct cgcgtgactc cagcaggaac agcaccgagc cgcagcgcgg gcagcccgcc
     1561 gccttcagtt gctcgatgct gggcgcggcg gcatcgggcg cctggcccac gcgcagcttc
     1621 gtgccgcagc ccatgcagcg gtacacgcgc acggtcgggt tcatctcgcc ggtatagagc
     1681 gggccgctga actggccggt gatcttgggc cggctggccg gcgtggtttg caggcggatc
     1741 atctggctca ccccggccag gatgcccgcc ccgcgatact cgttgaagtc gggaatgagt
     1801 tcggcaatcg ccaggcccat cgcggccagc gagccttgca gcgcggcctg ctcggcgggc
     1861 gggcggtggc cgaaggagag gaagatctgg cggccctgct ccggcaccag cgcgctcacg
     1921 gcccgcgaga cgaacaaatc cagcccgacc gcggtatacg gcggatcggt cacgacggtg
     1981 tcgaaggcgt ggagcaggtc ctccggcagc gggtcgcgca ggttgtgttc gaccgtctcg
     2041 atctccagcc ccgcctcctc ggcgcaggcg cgcagatggg ccaggatgcg cgggtcggtg
     2101 tccacgacgc agaggcggcg cgtcagccgc ccgccgccct gcgggctgag caactggccc
     2161 accaggccga tggcgaggct caccgagtcg tcgtcgccca gcagcagcac ggccttgccc
     2221 gccagcgcgc cctgtgtctg catatagagc acgcggcgca ggttcgtctc cggcgtgcaa
     2281 tggctctggt ccagcgtcac gtccacggtg gggttggcgg cgaaccacgc ctccagttgg
     2341 gccagcacgc cctcccactc gctgcccgcg accacgtgcc gcccctggca aagcgggcag
     2401 aggaagccct gccccaccca gccccaggcg ccttcgacca gtaccagccc ctgcggcgac
     2461 aggcggatat acggccccgg ctgcaccagc ccggccttct ccagttcgcg ccggatcgcc
     2521 gtgaccaccg ggatcggcag ccgcgtctgg cgggccagat ccttgccggt cagcccgccg
     2581 ctgcggtgga tcgtccgcag cacggcctcg atccccgccg cgccctcgcg cagccgcgtg
     2641 cgcgccgcca ggtcgctgag cagggtattc gccgcgccgg tcgccggctc tttattatcg
     2701 cccactacca atcgatcggt cctcgcctat ctgcaaaatc ccgcgccgtc cgcggcacac
     2761 cacgggcagc atagatcacc attataccat acgcctgttt cgggaacggc ggcgccgagc
     2821 cgcatcccgg tgggggacca acctaacccc ccctgccccc cttcccgccg cgggaagggg
     2881 aaatcggcgc tttggctcgg cgccaggcgt ggctacttgt gcttcttgtt cttcttacgc
     2941 gattctttgc ccatatggcg cttggactta gccttatcac caggcgatgc cccgcccgcg
     3001 ccggttcgcg ccgtggccga aggtcggaag ggcttgatgg acggggccgg ggcgctcccc
     3061 aacatttcct ggatgcgttg cacccgcagc gggtcggcca gcggctgatc cggatcgcgg
     3121 cccacttcct ccggtgtctt caagccgaac tcgatctgca cgtcgtccca ggtcgccatc
     3181 atgggaccga ccttgccggc cttgaagacc tgctcgatca gcgggagtgt ctcggcggct
     3241 tggagccgca tcaattcaga gataaggaag ccgttcaagt cctcgtcgtt agtcgcgtat
     3301 gcttccaact ggcgcacaat gggcgcaagg gccgcgtccc gcgcgtccgg atactgctca
     3361 gcaatcttgc ccagcgccgt aagcgcggac atgcgtccat agataccctg gctgtcgtcg
     3421 gccagcaagg cgctcagtgc gggaatcgcc gccgggccga tcatcccgta aacatccggc
     3481 aactcttcca ggatcgagtc gtcaaaccga tcctcatccc cggtcgtcaa
//
LOCUS       DASUYC010000353         2182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1631537, whole genome shotgun sequence.
ACCESSION   DASUYC010000353 DASUYC010000000
VERSION     DASUYC010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1631537"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            311..923
                     /locus_tag="VF276_05815"
                     /pseudo
     CDS             311..923
                     /locus_tag="VF276_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013477107.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
     gene            1019..1615
                     /locus_tag="VF276_05820"
     CDS             1019..1615
                     /locus_tag="VF276_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006746758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX6606418.1"
                     /translation="MSKVIAIMSMSLDGYVADRNDGVADVFDWYFTSGDVAFPAGGAD
                     PMTFQVSGPSAAHLRALWSELGAVLTGRRMFDGAGGWGGHHAWGPAFVLTHAIPAGWP
                     RPNSTVHFVTDSLESAVRQAKAAAAGKSVGVHGADTIQQLLNAGLLDELSVDIVAVLL
                     GAGVRLFDHLAGTPAVLGNPAGIPGAGVTHLRYPIRKA"
     gene            complement(1893..>2182)
                     /locus_tag="VF276_05825"
     CDS             complement(1893..>2182)
                     /locus_tag="VF276_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606419.1"
                     /translation="FLELPVGEERKAQVPAREGRLAATATFARDVLLRLDDNLRRAPA
                     LGVPDIPTPDLVRQARRTLRPAGEPLPLPPESLQRRAPRLGALDTPALAPA"
BASE COUNT          322 a          787 c          748 g          325 t
ORIGIN      
        1 tgtagtcgat ccaataagca gcacgcgcgc cgcaacggcc ccgccttacc gcttggagtg
       61 gggagtatcg ccgcatgggc ccggcggccc tcccgattct tgggcgcgcg caagtcacag
      121 ggcttgcgcg ggccggggtg cagcaccacc gggtaggcgc agtcgcacac tggtgctata
      181 ctagcaccct gggcccgagc tttcaatacg gccgataccc caccatatgc gggggtcgtt
      241 cggcttgcgg agccatacgc gggccgagcg cggcggtgcg gcgccctata ccgcggcaag
      301 gagcggaatc atgaaattct tgctcacctc ggcgggcatc acgaacccgt gcatccacca
      361 cgcgctggtc gacctcctgg gtaaaccgcc ggcccgctcc cgcgccctct gcattcccac
      421 ggcgatctac ccctgccccg gcgggccggc catggcctac cggttcatca gcggatccac
      481 cgcccacccc ctgggcgcct tgggctggca gtcggtgggc gtgctggaac tcacggccct
      541 ccctggcatc ccagaagcgt attggatcgc ggtggtcctg gcgacgggag tccgggccgg
      601 ccgcccgcct gccggcgcta cgcgcgaccg tctatgtggg ggtgagcgcc ggcagtatgg
      661 tgacggcccc gctgttcggc gcaagatacg acgacccgcc gccgcccttc gtcatcgcga
      721 cggggctggg cctggtggac tttgcggtgc taccgcccct ggatcacgcg catcaccccg
      781 aaagtgccac ggtcaaggtg gaacgcatgg ccaccgccgt acccgtgccg ctgtacgcga
      841 tcgacgatgc gaccgcgatc aaagtgaccg gcggcactgg ggaagtcgtg tccgcggggc
      901 actggaagct gtttaccgcc taatggaaaa gagtgctcct cccggagcct aacaaacgga
      961 tacacgcgcc ctcgccatcg tgcccagaga gcgcgcccgc acagaaagga gcagcgccat
     1021 gtcgaaggtt atcgcaatca tgtctatgtc cctcgacggc tacgtcgccg accgcaacga
     1081 tggcgtggcc gacgtgttcg actggtactt cacgtcgggg gatgtcgcat tccccgccgg
     1141 cggggcggac cccatgacgt tccaggtgtc cggcccgagc gccgcccacc tgcgcgcgct
     1201 ctggtccgaa ctgggcgccg tgctcaccgg gcgccgcatg ttcgacggcg ccggcggctg
     1261 gggcggccat cacgcctggg gacccgcctt cgtgctgacc cacgccatcc ccgccggctg
     1321 gccgcgcccc aactcgacgg tccactttgt gaccgacagc ctcgaaagcg ccgtgcgcca
     1381 agccaaagcc gccgccgccg ggaagtccgt cggtgtccac ggcgcggaca ccatccagca
     1441 gttgctgaat gccggactcc tcgacgagct cagcgtcgac atcgtcgcgg tgctgctggg
     1501 cgccggagtg cgcctcttcg accacctcgc cggcacccct gccgtgctgg gcaacccggc
     1561 gggcatcccg ggcgccggcg tgacccacct gcgctacccc atacgcaagg catagtccga
     1621 ccccccacct gggtcgccca ggggtgcgct ttgtcgcgat gcgacgtcca cgcgggaagc
     1681 gccaacgccg ggtccacaag gagcgcgcct gcctgccctg ggcgcagatc aggcggtaac
     1741 acccagcgct ctggctagca ggtgcgcgcc ggggtcgcgc acggcgacgt gcccgcgcgg
     1801 gtccgtctgg cgctacacgc gctttccgtt tgcccctcgt tgccgcggcg ttgggtttac
     1861 ccctactcaa gcgactatag tcgacgggag tcttacgcgg gcgcgagcgc aggggtgtcc
     1921 agagcgccga ggcgcggcgc gcgccgctgc agggattccg gcgggagcgg cagcggctcc
     1981 ccggcggggc ggagggtgcg gcgggcctgg cggaccaagt cgggggtcgg aatatcgggc
     2041 acgccgaggg ccggggcgcg gcgcaggtta tcgtccagcc ggagtagcac atcgcgggcg
     2101 aaggtggcgg tggccgcgag ccggccttcg cgggccggca cctgggcctt gcgctcctcg
     2161 cccaccggca actccaggaa ct
//
LOCUS       DASUYC010000354         4411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_262476,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000354 DASUYC010000000
VERSION     DASUYC010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262476"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1386)
                     /locus_tag="VF276_05830"
     CDS             complement(<1..1386)
                     /locus_tag="VF276_05830"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEX6606420.1"
                     /translation="MYAFGVDYYPEHWPEERWPIDARLMAEAGFNIVRLAEFAWSRLE
                     PQEGRFDFAWLDRAIAGLVAQGIRVILGTPTASPPPWLMEAHPDLYRVDDQGRRLTFG
                     NRREYCPNHPLYHDYTRAIVTAMAAHYAEHPNVVGWQIDNEFGDHCYCPVCRRAFQDW
                     LRHRYAGLDALNAAWGTIFWSHVYTDWAQIPVPAATGGSPNPGLALDFARFASDSYVA
                     YQQLQIDILRGHTAAQFITHNLMGFKYNKINYYDLARSLDLVSWDNYPRTQWSFTAEV
                     DPSGPALAAATMYGLKGQPFWVMEQQAGPGGWEMVSAMPRPGELRLWAYQQIAHGADG
                     LVFFRWRTARYGTEQYWHGLLEHDGRPSRRYEEVARLGAELRRAGERISASRVEAQVA
                     LLLSYDSRFAFQIQPNNPGYSYAEHFHALYRTFYERNVAVAVVDPASDLSGYRLVLAP
                     PLYVLAPAVAAN"
     gene            complement(1413..2270)
                     /locus_tag="VF276_05835"
     CDS             complement(1413..2270)
                     /locus_tag="VF276_05835"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX6606421.1"
                     /translation="MARTAIVPPRPARSMVPRIVVYIALLLLSAFMIFPFLWMLVSSF
                     KPFAEIFAGTTFLPQHPTLHNYISLFDQSDALRKIWNSFYIAATSTVLSVFLCALGGY
                     AFAKFQFPGRAFLFSFMLASMAIPFAVVMVPLFIMMRNTFHWIDTPLPLIIPGAANAF
                     GIFFMRQYMSAMPNEMLDAARVDGASEFRIFLRIVLPTSIPGLVSLGIIFFMSSWNNF
                     LWPTAVLRSAAQQTVPLMLNSLQGPPGRTAFDVLMAGSVISLLPMLIVFLVLQRHLIA
                     GITAGSIRG"
     gene            complement(2273..3244)
                     /locus_tag="VF276_05840"
     CDS             complement(2273..3244)
                     /locus_tag="VF276_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX6606422.1"
                     /translation="MRNSPPQPELSKTRVPATRAEWVAGAAGVRPRNPLARLWRFRFP
                     LLFISPFFILFVIFGVYPIIFSLWLSLHSWRGVGDMKFVGLDNYVLLLRDRIFWNTML
                     NSAILFFIYVPLMTFLACVLATILHADFVKLQGLWRALIFVPYITSMVATGYTFKLIF
                     DQRAGIANHFLGFVGIGPIPWLDDPWWARITLGLLMIWAWLGYNTVIMLAGMQTIPSE
                     IGEAALVDGASRTQIFFRITIPLLRPVIIFAVTLSIIGTFQMFTEPYILTSGGPIRAT
                     ETPMMEIFSNTFTALKFGYAAAMSYVYFAIIVVITLAQLRLVTRRES"
     gene            complement(3258..>4411)
                     /locus_tag="VF276_05845"
     CDS             complement(3258..>4411)
                     /locus_tag="VF276_05845"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6606423.1"
                     /translation="NGLKVVDADFRQAYPNVDVEYVARQPSETYQQIQLAASAGTGFP
                     DISLIEDSHLAQFVSLGILADISDKAQPYVPQFNAYRWQQAMAGGKYYAMPWDSGPVA
                     IFYRRDVFTKAGVDPDSIKTWDDYYQAAKTIKAKAGVPMWQQAKAQNDGRLFEMLMWQ
                     QGLGYVDKDGNVILDKDPQIQQILEYIGKFWQEDLAADTQPWTDAWYKEMADGTVATV
                     PEAVWMGTFFKSFIAPDAAGKWGIFKLPAWQAGGVQSANDGGSSLAIFEGSKQKDAAW
                     AYVQFHLGLPAEQVAIYKQTDLFPGLQSTYSDPAFQEPDPYFDGQKARAFFTDVAKDI
                     PQAGVYSTDYQQMNSLLTPEIQKFALGQQSAKDALANAAGAIRDRTGRK"
BASE COUNT          881 a         1323 c         1457 g          750 t
ORIGIN      
        1 attggccgcc acggcggggg ccaggacgta cagcgggggc gccagcacga gccggtaacc
       61 gctcaggtcg gatgccggat cgaccacggc gacggcgacg ttgcgctcgt agaacgtgcg
      121 gtatagcgcg tggaaatgct cggcgtagct atagccgggg ttgttgggct ggatctggaa
      181 cgcgaaccgc gagtcgtagg acagcagcag cgccacctgc gcttcgactc gggacgccga
      241 aatccgctcg ccggcccggc gcagttcagc gccgaggcgc gcgacctcct cgtagcgccg
      301 gctagggcgg ccatcatgct ccaacaggcc atgccagtat tgctcagtac catagcgcgc
      361 cgtgcgccag cggaagaaga ccaggccgtc ggcgccgtgg gcgatctgct ggtacgccca
      421 gagccgcagt tcgccggggc ggggcatggc gctgaccatc tcccagccac cggggccggc
      481 ctgctgctcc atcacccaga acggctggcc tttcaggccg tacatcgtgg ccgccgccag
      541 cgccgggccg ctcggatcga cctcggccgt gaacgaccac tgtgtgcgcg ggtagttgtc
      601 ccagctcacc agatcgagcg accgcgccag gtcgtagtag ttgatcttgt tgtacttaaa
      661 gcccatcaga ttgtgcgtga tgaactgcgc tgctgtatgg ccgcgcagga tgtcaatctg
      721 gagttgctgg taggccacgt acgagtccga agcaaagcgg gcaaaatcga gggccagacc
      781 gggatttggc gatccgccgg ttgccgcggg cacggggatc tgcgcccagt cggtgtaaac
      841 gtggctccag aagatcgtgc cccaggcggc gttgagcgcg tcgaggccgg cgtagcggtg
      901 ccgcagccag tcctgaaacg cgcggcggca caccgggcaa taacagtggt cgccgaattc
      961 gttgtcgatc tgccacccta ccacgttggg atgctcggcg taatgtgcgg ccatcgcggt
     1021 gacaatggcc cgcgtgtagt cgtggtacag cggatggttc gggcagtact cgcgccggtt
     1081 gccaaaggtc agccggcgcc cctggtcgtc gacacggtac aggtcggggt gcgcctccat
     1141 cagccagggc ggcggcgagg cggtcggcgt accgaggatg acccggatgc cttgggcaac
     1201 cagccccgcg atggcccggt cgagccacgc gaagtcgaag cggccttcct gcggctccag
     1261 tcgcgaccac gcgaattccg ccaggcggac gatattgaag ccggcctccg ccatcaaccg
     1321 ggcgtcgata ggccagcgct cttccggcca gtgctcgggg tagtagtcaa caccaaaggc
     1381 gtacatacat gattcctctc gtcaggctag gctcagcccc tgatcgatcc tgcggtgatt
     1441 ccggcaatca agtgccgttg cagcaccagg aagacgatca gcatgggcaa gaggctgatt
     1501 accgagccgg ccatcagcac gtcgaaggcc gtgcggccgg gcggcccttg taacgagttg
     1561 agcatgagcg gaacggtttg ctgggccgct gagcgcagga cggcggtggg ccacaggaag
     1621 ttgttccagg aactcatgaa aaagataatg cccaggctaa ccaggccggg gatgctggtc
     1681 ggcaacacga tgcgcaggaa gatccggaac tccgacgccc cgtccacccg cgcggcgtcc
     1741 agcatctcgt tgggcatcgc ggacatgtat tgccgcatga agaagatgcc gaaggcgttg
     1801 gccgcgccgg ggatgatcag cggcaggggc gtgtcgatcc agtgaaaggt gttgcgcatc
     1861 atgatgaaca ggggcaccat caccaccgcg aagggaatcg ccatcgaggc gagcatgaag
     1921 ctgaacagga aggcgcgccc cggaaactga aacttggcga aggcatagcc gcccagggca
     1981 cacagaaaca ccgacagcac tgtgctggtc gcggcaatgt agaagctgtt ccagatcttg
     2041 cggagcgcgt ccgattggtc aaacagggag atatagttat gcagcgtcgg gtgctgcggg
     2101 aggaacgtgg tgccggcgaa gatctcggcg aacggcttga acgacgaaac cagcatccag
     2161 aggaaaggga agatcatgaa ggcgctcaac agtagcagcg caatgtagac gacgatacgt
     2221 ggcaccatgc tgcgcgcggg gcgcgggggc acgatcgcgg tacgggccac ggttaagact
     2281 cccttcgagt caccagccgc agttgggcga gggtgatcac cacgatgatc gcaaagtaca
     2341 catagctcat cgcggcggcg tagccaaact tcagcgcggt gaaggtgttg gaaaagatct
     2401 ccatcatcgg cgtttcagtg gcgcggatcg gcccgccgct tgtcagaatg tagggttccg
     2461 tgaacatctg gaaggtgccg ataatcgaca gcgtgacggc gaagataatc accggccgca
     2521 gcagcgggat cgtgatccgg aagaagattt gtgtgcggga ggcgccatcc accagcgcgg
     2581 cctccccgat ctcgctgggg atggtttgca tgccggccag catgatcacg gtattgtatc
     2641 ccagccacgc ccagatcatc agcaggccca gcgtaatgcg cgcccaccag ggatcgtcga
     2701 gccaggggat cggcccgatg ccgacgaaac ccaggaagtg attggcgatg cccgcccgct
     2761 ggtcgaagat gagcttgaac gtgtagcccg tcgccaccat cgaggtgata tagggcacga
     2821 agatcagcgc gcgccagagg ccctgcaact tgacgaaatc ggcatgtaag atcgtagcta
     2881 gcacgcaggc cagaaacgtc atcagcggga cgtagataaa aaacaggatg gcgctgttca
     2941 acatcgtgtt ccagaagata cgatcgcgca acaggagcac gtagttgtcc agacccacga
     3001 atttcatgtc gcctaccccg cgccagctat ggaggctgag ccacagggag aagatgatgg
     3061 gatagacccc gaatatcacg aagaggataa aaaacggcga gataaacagc agcggaaacc
     3121 ggaaacgcca gagccgggcg agcggattac gcgggcgcac gcccgccgcg cctgccaccc
     3181 actcagcgcg ggtggcaggc acgcgagtct tcgatagctc cggctgcggc ggtgaattgc
     3241 gcatgcggag gctccctcta cttgcgacct gtcctgtcgc ggatcgcacc ggcggcgttg
     3301 gccagcgcgt ccttggcact ctgctggccc agggcgaatt tctggatctc cggcgtcagc
     3361 agcgagttca tctgctgata gtcggtactg tagacgcccg cctgcggaat atccttggcc
     3421 acgtccgtga agaaagcccg ggccttctgg ccgtcgaagt agggatcggg ctcctggaac
     3481 gccgggtcgc tgtaggtgct ttgcaggccg gggaacaggt cggtttgctt gtagatcgcg
     3541 acctgctcgg cgggcaggcc cagatggaac tgcacgtagg cccaggcggc gtccttctgt
     3601 ttgctaccct cgaaaatggc gagcgacgat cccccgtcat tggccgattg gactccgccc
     3661 gcctgccagg ccggcagttt aaagatgccc cacttgccgg cggcatcggg cgcgatgaag
     3721 ctcttgaaga acgtgcccat ccagacggcc tcgggtaccg tggccacggt gccgtccgcc
     3781 atctccttat accaggcgtc ggtccagggc tgggtgtcgg cggccagatc ctcctgccag
     3841 aacttgccga tatattccag aatctgctgg atctggggat ccttgtccag gatgacgttg
     3901 ccgtccttgt cgacatagcc caggccctgt tgccacatca acatctcgaa caggcggccg
     3961 tcgttctggg ccttggcctg ctgccacatc ggcacgcccg ccttggcttt gatcgtcttg
     4021 gcggcctggt aataatcgtc ccacgtcttg atggaatcgg gatcgacgcc ggctttggtg
     4081 aagacatcgc gccggtagaa aatcgccacg ggcccgctgt cccagggcat ggcgtaatac
     4141 ttgccgccgg ccatcgcctg ctgccagcga tacgcgttga actggggcac atagggctgc
     4201 gccttgtcgc tgatatccgc caggatgccc aggctcacaa actgggccag gtgggagtcc
     4261 tcgatgagcg aaatatcggg gaacccggtg ccggccgagg ccgcaagctg gatctgctga
     4321 taagtctcgc tgggttgccg cgcgacatac tcgacgtcga cattggggta ggcctgccgg
     4381 aaatcggcgt ccaccacctt gagtccgttc a
//
LOCUS       DASUYC010000355         5994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1758090, whole genome shotgun sequence.
ACCESSION   DASUYC010000355 DASUYC010000000
VERSION     DASUYC010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5994
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1758090"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2848)
                     /locus_tag="VF276_05850"
     CDS             complement(<1..2848)
                     /locus_tag="VF276_05850"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX6606424.1"
                     /translation="MATLHKRVGKMLLAAGLALSVVLPAGAADRLPPAGAPIIAGGVQ
                     VTDAPVTNPQPAARILDKGDPNQLVDAIVLLKDPPLAEVYKQTEATQGRAAAEKADYQ
                     RTYSRQLLAAQAPVAAAAARFGIVTNHMTVVLNGLAMTSVHRRDLAALRQIPGVAHVS
                     EGANYKLDLYLSVPFIGADAVRLGMGLDGRGTRVAVIDTGIDYTHRNFGGPGTVAAFD
                     YAVAHATQAPPIVYSGTVLFPTQKIPGGKDFVGDAWDGSTVPTRTVDLNPLDTPPEGG
                     HGSHTSGIVGGFGVPNISVDGSIPPNPTDFGGCTTPACADPPLYHGVAPAAQIYMYKV
                     CSSHVNSCSGVAMMLGVEASVDPNGDGVITDHMDAANMSIGQSFGIGNDEIATAIDNA
                     VAVGVAYAVSAGNSSNVPFITGAPATAAKALSVAASTASGHFNYALDISQPVTMTTNV
                     VETVWQPWSAPLTPPTTGDLIDARVAFPANPIGCNAFPAGSMTGKIAVIDRGTCAVSL
                     KAQNAQAAGAVGMILVLLPGQELTSFSFGGGPAVTIPVVVARNSEMAPLRAYMVAGGT
                     VTVTLGTSNPVPQDDVLAGFTSRGPALQEILKPDISAPGVDIVSTGYGTGTAGVFMSG
                     TSMAAPHIAGSLALMKQAYPAWSAEDREAALVNGSKMGLYRTAVDRANGITAPVSLAG
                     AGRVALTTTVPLSTTVIGSDINVNFGIPVIAQTLNASRVVTVTNHSAVAKSYTLSATT
                     QYTQPAALTNLSVTPATLNVPAGGSATALLNLTINPALLPSWGLSGTGGASSAALSAA
                     EIGGFLFVNDAAANQQAHLAWYVLPRPASNTTAAPASLSYGGGVVSDTITLTNTGAIS
                     GTTEVFYLAGRDPHENSATNPLLGGDRDDIEYTGVRSISASGPVTDSVQFAVHTYAPR
                     VLQLEAEFDIGVDTNRDGTDDYVVFNYDIGAFNTG"
     gene            3400..4668
                     /locus_tag="VF276_05855"
     CDS             3400..4668
                     /locus_tag="VF276_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606425.1"
                     /translation="MATALLICWAAGGPATPGGLAAGLPQAPASPTAVPPGSWPAAAN
                     AGIASARDDLARRLNAGPAEITVVSVTAQEWPSSAMGCPLPRQNYLPVITPGYEIILA
                     AGGAHYTYHASLRGAVVACPASTVPPPPAAVRYFPETGHSVQGRFLAYWEQRGGLAQQ
                     GYPLTDELQEVSAADGKTYTVQYFERDVFELHPENQPPYDVLLSRLGAARWAARYPTP
                     PANQRASTDNPRRFPETGHTIGGRFRAYWEQHGGLAQQGYPLSEEFQEQSDLDGRTYS
                     VQYFERAVFEYHPENPPPYDVLLTQLGKFHLAISRAQAVAAGLKSPPRVYAVGPNDIV
                     LWPPDTGSAVTHYSTCGIQPLAWALADACQQPMTLTTALAGAGDGLDVTFLATWASGA
                     RIHRWQLHVGSDLHAVFVQESGDRLPILPQ"
     gene            5284..>5994
                     /locus_tag="VF276_05860"
     CDS             5284..>5994
                     /locus_tag="VF276_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606426.1"
                     /translation="MRNLPARSRPGLLFAALLLAALGIALGGIRWQGEQQTSPADAAR
                     AKVKANFAQIADSIEGHSGTEAAQSGQAPREEDLTKRADYWTQRVTYPTGQYDPAWLL
                     QAAAQDKQIESRVPAGQTTYQQERANGSLALNPSSWVPLGPAPAQSDGCWSCFNFGLV
                     GGRVNDIAIDPVISTTAYIATVGGGVWKSTNCCAADTGWTSTINDPLINEVGISDLAI
                     DPNNHNTIYAGTGEHSVAT"
BASE COUNT          955 a         2018 c         2017 g         1004 t
ORIGIN      
        1 tgcccgtgtt gaaggccccg atatcgtagt tgaacacgac atagtcgtcg gtgccgtcgc
       61 ggttggtatc cacgccgatg tcgaactcgg cctccagttg cagcacgcgc ggcgcgtagg
      121 tatgcaccgc aaactgcacg gagtcggtca cggggccgct ggcgctgatc gagcgcacgc
      181 cggtgtactc gatgtcgtcg cggtcgccgc cgagcagcgg gttcgtggcg ctgttctcat
      241 gcgggtcgcg gcccgccagg tagaacacct cggtggtgcc cgagatggcg ccggtgttgg
      301 tcagcgtgat ggtgtcggac actaccccac cgccatagct caggctggcg ggcgcggctg
      361 ttgtgttcga ggcgggccgc ggcaggacgt accaggccag gtgcgcctgc tggttggccg
      421 ccgcgtcgtt cacgaacagg aagccgccga tctccgctgc cgacagcgcc gcgctgctgg
      481 cgccgcccgt gccgctcagg ccccaagacg ggagcagcgc cgggttgatc gtcaggttga
      541 gcagggccgt cgcgctgccg ccggccggca cgttcagggt ggccggggtc acgctgagat
      601 tggtcagggc ggcgggctgg gtgtattgcg tggtcgcgct gagcgtatac gacttggcga
      661 cggcgctatg gttcgtcacc gttaccacgc ggctggcgtt cagcgtctgc gcgatcaccg
      721 ggatgccgaa gttgacgttg atgtcactgc cgatcacggt cgtgctgagc gggaccgtgg
      781 tggtgagtgc cacacgcccg gcgccggcca ggctcaccgg ggcagtgatg ccgttggcgc
      841 gatccacggc cgtccggtac aggcccatct tggacccgtt caccagcgcc gcttcgcggt
      901 cttccgccga ccaggccgga taggcttgct tcatcagggc cagcgacccg gcgatatgcg
      961 gcgccgccat cgacgtaccc gacatgaata cgcccgcggt acccgtgccg tatcccgtcg
     1021 agacaatgtc cacgcctggc gcgctgatat ccggcttcag gatttcctgc agcgccgggc
     1081 cgcggctggt gaagccggcc agcacgtcat cttgcgggac ggggttggat gtaccgagcg
     1141 taacggtgac ggtgccgcct gcgaccatat aggcccggag cggcgccatt tccgaattgc
     1201 gcgcgaccac gaccgggatc gtgaccgccg ggccgccgcc gaaggagaag ctagtcagtt
     1261 cctgacccgg cagcagcacg aggatcatgc cgacagcacc cgccgcctgg gcgttttgcg
     1321 ccttcaaaga cactgcgcaa gtgccgcggt cgatcaccgc gatcttaccg gtcatggaac
     1381 cggccgggaa cgcgttgcag ccgatggggt tcgctgggaa ggccactcgc gcgtcgatca
     1441 ggtcgccggt ggttggtggc gtcagcgggg cggaccaggg ctgccagacc gtctcgacca
     1501 cgttggtggt catagtcacg ggttggctga tatcgagcgc atagttgaag tgcccgctgg
     1561 ccgtcgaggc cgccacggac agggccttgg ctgccgtggc tggcgcgccg gtgatgaacg
     1621 gcacgttgct ggagttgccg gccgacaccg cataggccac gcctaccgcc accgcgttgt
     1681 cgatcgcggt cgcaatctca tcgttaccga ttccgaacga ctgcccgatc gacatgttgg
     1741 cggcgtccat gtggtcggtg atcacgccgt cgccgttggg gtcgaccgag gcttccacgc
     1801 cgagcatcat cgccacgccg ctgcaactgt tgacgtggct ggaacagacc ttatacatgt
     1861 agatctgggc cgcgggggcc acgccatgat acagcggcgg gtccgcgcag gccggcgtgg
     1921 tgcagccgcc gaagtcggtg gggttcggcg ggatgctgcc gtccacggag atattcggca
     1981 cgccgaagcc gccgacaatc ccggaggtgt gcgagccgtg gccgccttcc ggcggcgtat
     2041 ccagcgggtt cagatcgacg gtgcgcgtgg ggaccgtgct accatcccac gcgtcgccca
     2101 cgaagtcctt gccgcctgga atcttctgcg tggggaatag caccgtgccc gagtagacga
     2161 tgggcggggc ctgggtggcg tgcgcgacgg catagtcgaa tgccgccacc gtgccgggac
     2221 cgccgaagtt gcggtgcgtg taatcgatgc cggtgtcgat gacggcgacg cgggtaccgc
     2281 gcccgtcaag ccccatccct aaccggacgg catccgcgcc gatgaacggc acgctgaggt
     2341 agagatcgag cttatagttc gcgccttcgg agacgtgcgc gacccctggg atctggcgca
     2401 gagccgccag atcgcggcgg tggaccgagg tcatggcgag gccgttcagc accaccgtca
     2461 tatggttggt gacaatccca aagcgggcgg ccgcggcggc gacgggcgct tgtgcggcca
     2521 gcaactgccg gctgtacgtg cgctgatagt cggccttctc ggcggcggcc cggccctgcg
     2581 tggcctcggt ctgcttgtaa acctccgcca gcggcgggtc cttcaagagc acaatcgcat
     2641 cgacgagctg gttcgggtcg cccttatcca gtatccgcgc cgcgggctgg gggttggtga
     2701 ccggggcgtc cgtcacctgc acacccccgg cgataattgg cgcgccggcg ggcggtagtc
     2761 tatcggcggc gccggcgggt agcacgacgg acagtgccag gcccgcggcc agcagcattt
     2821 tgcccactcg tttatgcaga gtagccaatc taattctcct tcccgtgcat tcgaggtgga
     2881 aacgggactc ccttctccgt gccggtggcg ccagacgagc agaaaccgcg cgcctgcgac
     2941 ggatcgggga tgcttctcct acttctgtag tggtctgcgg caggcatgtt gggatgggga
     3001 tgcgcccgca gcggggcgcg gaccaacctg ccgggatgaa gagtggtgcg gggagtagcg
     3061 cttggtgcct ccagcagatc tcctttcgtc attgattgga gtagcctggc gcgtaacacc
     3121 accgccgggg ttcgtaagag acagcgggtt acgacgcgtg tattataggc ggactccgcg
     3181 gcagtgtcaa cgccctgaaa atgcgccagg gcgttataat ataatgcagg gtgcgcgggt
     3241 gcctgattcg accggcggat gccggttgcg tactctgtta aggcgcgccg ccgggtgtat
     3301 gtggcgcacc acggcgttca agggcgatag gatcgctttc cgggggaggt ggcggggatg
     3361 ccggcgaatg gctatcgatg gttatgggga ccggttggtt tggcgacggc actgcttatc
     3421 tgttgggccg cgggcggccc ggccacgccg ggcgggctgg ccgccgggct gcctcaggcg
     3481 ccggccagcc ccaccgcggt gccgcccggc tcctggccgg cagcggccaa cgccgggatc
     3541 gccagtgccc gtgatgacct ggcccggcgc ctcaacgccg gcccggcgga gatcacggtg
     3601 gtcagcgtca ccgcgcagga gtggccgagt tcggccatgg gctgtccgct gccccggcaa
     3661 aactatctcc cggtgatcac gcccggttac gagatcatcc tcgcggcggg gggcgcccac
     3721 tatacctatc atgcgagcct gcgtggagca gtcgtcgcct gcccggccag tacggtgccc
     3781 ccgccgcccg cagccgtgcg ctatttcccc gaaaccggcc atagcgtcca gggccggttc
     3841 ctggcctact gggagcagcg cggcggcctt gcccagcagg gctacccgct caccgatgaa
     3901 ctccaggaag tctccgccgc cgacggcaag acctacactg tgcagtattt cgagcgcgac
     3961 gtgttcgagt tgcatcccga gaaccagccg ccttacgatg tgctgctcag ccgcctgggc
     4021 gcggcgcggt gggccgcccg ctacccgacc ccgcccgcga accagcgcgc gagcaccgat
     4081 aacccgcgcc gctttcccga gaccgggcac accatcggtg gccggttccg cgcctactgg
     4141 gagcagcacg gtggcctggc gcagcagggc tacccgctca gcgaggaatt tcaggagcag
     4201 agcgatctcg acgggcggac ctacagcgtg cagtatttcg agcgcgcggt ctttgagtat
     4261 caccccgaga atccgccgcc ctacgatgtg ctgctcaccc agctcggcaa gttccacctg
     4321 gcgatcagcc gcgcccaggc cgtggccgcc ggcctgaaat cgccgccgcg cgtctacgcg
     4381 gtgggcccga acgacatagt cctctggccg cccgataccg gatcggccgt cacccactac
     4441 agcacctgcg gcatccaacc cctcgcctgg gccctggccg atgcttgcca gcagccgatg
     4501 acgttgacca cggcgctggc cggggcgggc gacggcctgg acgtgacctt cctggcgacc
     4561 tgggccagtg gcgcccggat ccaccgctgg caactgcatg tgggctccga tctccatgcg
     4621 gtattcgtgc aagagagcgg ggaccgcttg ccgatcctgc cgcagtaagc gggagcaggc
     4681 ggcggggggt acgacccgcc gcccgctcgg cgggccgatt cccggcgaga ccctattact
     4741 ggcgcacaaa aacatacttt ttcacttgca cgaggttcta agctgtaata taatcgcatt
     4801 acgtgttatt tcaccgctcc cattggcgcg gccggcccgt ggctccgttt gcttccgcag
     4861 gggtaccggg ggccgccggc caacatagcg gaaaggaggt gggccggagt ctgctgcgct
     4921 gctccccgcg caaataagca cctgctgcat cgctgggccg gtccacgctt cccagcaccg
     4981 agatgcatct accactgcag gtgcgctacc ccattatcgc cgggcaggtc cacgctccgc
     5041 ccccaatata tgcatctatc ggactagatg cgacacctcg tccgcgccgg gccgctccac
     5101 gcaccgcccg cgcccggatg cgcctaccag gataggcgcc cacgcccacg cttgccgggc
     5161 cagtccacgc tgcgcccgta accaaatgca ccggccacgg ccggcgccat actctattgc
     5221 cgccgggccg ctccacgcgc cgcccgccgg cgatagcctg ttcttctgaa ggagagagat
     5281 ccaatgcgca acctacccgc aaggtcgcgg cctggtttgc tttttgccgc gctgctgctg
     5341 gcggcgctcg gcatcgccct gggcggcatc cgctggcaag gggagcagca aacaagcccc
     5401 gcggatgccg cgcgcgccaa agtcaaggcc aatttcgcgc agatcgccga ttcgattgag
     5461 ggccactccg gcaccgaggc cgcccaaagc ggccaggccc cacgtgagga agacctgacc
     5521 aagcgcgccg attattggac ccagcgcgtc acctatccca ccgggcaata cgatcccgcc
     5581 tggctactcc aggccgccgc gcaagacaaa cagatcgagt cgcgggtgcc cgccgggcag
     5641 acgacgtatc aacaggagcg ggcgaacggc tcgctggccc ttaaccccag cagttgggtg
     5701 ccgcttggtc ccgcgccggc ccagtccgac ggctgctggt cctgctttaa cttcggcctc
     5761 gtgggcgggc gcgttaacga catcgccatc gaccccgtca tctccaccac ggcctacatc
     5821 gccaccgtcg gcggcggcgt ctggaaatcc accaactgct gcgccgccga caccggttgg
     5881 acctccacca tcaacgaccc gttgatcaac gaggtgggta tctccgacct ggcaattgac
     5941 cccaataacc acaacacgat ttacgcgggc accggtgagc acagtgttgc cacc
//
LOCUS       DASUYC010000356         4322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1107019, whole genome shotgun sequence.
ACCESSION   DASUYC010000356 DASUYC010000000
VERSION     DASUYC010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1107019"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VF276_05865"
     CDS             <1..287
                     /locus_tag="VF276_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HEX6606427.1"
                     /translation="TRIGPAQQIEVEVTTPAELDEALAAGAELILLDNHTLDGLRAAV
                     AHTAGRARLEASGGITLDNLAAVAGTGVDFISLGALTHSAGAIDFSLEVL"
     gene            287..1306
                     /gene="nadA"
                     /locus_tag="VF276_05870"
     CDS             287..1306
                     /gene="nadA"
                     /locus_tag="VF276_05870"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEX6606428.1"
                     /translation="MATSTTTPRPLHPALRRFVPQDPAEIAALQAEVRALAKARNAVI
                     LAHNYQVPEVQDVADFVGDSLGLAIEAAGTAAPVIVVCGVYFMAETAKILNPERTVLI
                     PDVTAGCSLADSITVEQLRAWKAEHPGAVVVSYVNTTAAIKAESDYCVTSGNAQAVIR
                     AIPEDQEILFLPDVFLGTWLKKSLGRDNMEVWMGECHVHAGIRPDEILANQQAYPDAD
                     LLIHPECGCTSRYVWARNEGTLPAASTFVLSTEGMVRHAAQSPNTTHLVATETGLLHR
                     LRKENPGKTFVPADDGAVCRYMKQITLEKLRDTLRDMAPAVEVAPEVAAKARLAIDRM
                     LAIRG"
     gene            1460..2026
                     /locus_tag="VF276_05875"
     CDS             1460..2026
                     /locus_tag="VF276_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018561112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX6606429.1"
                     /translation="MTMRTGSGMEGKQMIKRNSHCGWCGHEYEGDPPWPRRCSACGHT
                     SYLNPLPVAVILVPVDNGVLAIRRTVAPCVGMLALPGGYINLGETWQEAGAREVWEET
                     GLRLDPAEIRDFAVLTADGYLLVFGLAVPHTAADLPPFTPTNETSERVVLPGPTDLAF
                     SLHTQALAMYFAARAGVGPVPPAAGSRA"
     gene            complement(2038..3126)
                     /locus_tag="VF276_05880"
     CDS             complement(2038..3126)
                     /locus_tag="VF276_05880"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEX6606430.1"
                     /translation="MGDRILVFGAGGYSGRMVVEELARRGQPMVLADRQAARMEALIA
                     EFGLDPDVPRHVADVDHPETLPPMFEDGVRTVINCVGPFLRLGEPVVAAAVAAGVHYL
                     DLSGEPPFIRQIIERYDAAARERGCVVVPTCGTEYALSNWAVALAARGLEPVDHIATT
                     FGITRDRPSKGTQRSFFLALAQPTQGWRGGKQVPYPPARQMRRVRLPAPFGRRLALNS
                     PLGELIQIPRHIQVTSMDSYLALPTPVALFLGALGPALPKVSPLIGRIIEPITRNPPP
                     DFVEQSICVVKATARSGKGRRNVILMGRQVYRLAAAIVCWCALQTLEPGFTARGVLGP
                     AQAFDPCAAVQALESAGLHHSPLPPGVC"
     gene            3373..3804
                     /locus_tag="VF276_05885"
     CDS             3373..3804
                     /locus_tag="VF276_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018885266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606431.1"
                     /translation="MCRYAEHVYKMPFACFACRKVFKQPSYWDLSPAQRPAPGQPRTI
                     PCPQCRRPMHAMGHDFRAPRQRDIAQWAKVAVLFRHGITYHSCGCNGPGPRPATLREV
                     PEFLADHLPQSPGARLLAQWQPSAERPQRRSRGGGSRLHAR"
     gene            complement(3817..>4322)
                     /locus_tag="VF276_05890"
     CDS             complement(3817..>4322)
                     /locus_tag="VF276_05890"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX6606432.1"
                     /translation="RQWVAAHRLGELDDAQACDPLLRVAEDAAQPPLVRGEALKALAR
                     LGDTRAVPVALRVIQESGGVSAGMTPAAPTMLHVQAIEVVGTIGDATATPILIGQLDD
                     GNAYVRYAAVQALGAAGTPESIPALERIQQTDPATITADSTGRRQARLRSAAATALAA
                     IRARYPA"
BASE COUNT          657 a         1516 c         1492 g          657 t
ORIGIN      
        1 gcacgcgcat cggcccggcc cagcagatcg aggttgaggt cacgaccccg gcggaactcg
       61 acgaagccct ggccgcgggt gccgaactga tcctgctcga caaccatacg ctcgacggcc
      121 tgcgcgccgc cgtggcgcat acggccgggc gtgcccggct cgaagccagc ggcggcatta
      181 ccctggacaa tctggccgcg gtggccggaa ccggcgtaga ctttatttcc ctcggcgccc
      241 tgacccactc ggcgggcgcg atcgacttct ccctggaggt gctctaatgg cgacttctac
      301 gacgactccg cgcccgctgc atcccgcgct acgccgcttc gtgccgcagg acccggcgga
      361 gatcgcggcg ctgcaagcgg aggtgcgcgc gctggcgaag gcgcgcaacg cggtcatcct
      421 ggcccataat taccaggtgc ccgaagtgca ggacgtggcc gatttcgtgg gcgactcgct
      481 gggcctggcg attgaggcgg cgggcacggc ggccccggtc atcgtggtct gcggcgtcta
      541 cttcatggcc gagacggcca agatcctcaa cccggagcgc accgtgctga tccccgacgt
      601 gacggcgggc tgctcgctgg ccgactcgat caccgtcgag cagttgcgcg cgtggaaggc
      661 cgagcaccca ggcgccgtgg tcgtcagcta cgtgaacacc acggcggcga tcaaggccga
      721 gtccgactat tgcgtgacca gcggcaacgc ccaggcggtg atccgcgcca tccccgaaga
      781 ccaggagatc ctgttcctgc ccgatgtctt cctgggcaca tggctcaaga agtcgctggg
      841 ccgtgacaac atggaagtct ggatgggcga gtgccacgtc cacgcgggca tccgccccga
      901 cgagatcctc gccaaccagc aggcatatcc cgacgccgac ctgctcatcc acccggagtg
      961 cggctgcacc agccgctatg tctgggcgcg caacgagggc acgctgcccg cggcgagtac
     1021 cttcgtcctc tcgaccgagg gcatggtccg ccacgccgcg cagtcgccaa acacgacgca
     1081 cctggtggcg accgagaccg gcctgctgca ccggctgcgc aaggagaatc ccggcaagac
     1141 gttcgtgccc gccgacgacg gcgctgtctg ccgctacatg aagcagatca cactcgaaaa
     1201 gctgcgcgac acgctgcgcg acatggcgcc cgcggtcgag gttgcacccg aagtcgccgc
     1261 gaaggcccgc cttgccatcg accggatgct ggcgatccgc gggtaagcgc ggcgaccccg
     1321 gtgcggagta cgccttccgg tggtacttgt cactctgagc ggagcgaaga gtctcgtcgc
     1381 gtgtggtact tgtcactctg agcggagcga agagtctcgt cgcgtggggc aagagacgag
     1441 atgcttcact gcgttcagca tgacaatgcg gactgggagc ggcatggagg gcaagcagat
     1501 gatcaagcgc aactcgcact gcggctggtg cgggcacgaa tacgagggcg acccgccctg
     1561 gccgcgccgc tgtagcgcct gcgggcacac cagctacctg aacccgctgc cggtcgccgt
     1621 gatcctggtg ccggtggaca acggcgtgct ggcgatccgg cgcacggtcg cgccctgcgt
     1681 cgggatgctg gccctgccgg gcggctacat caacctcggc gagacgtggc aggaggcggg
     1741 cgcgcgcgaa gtctgggaag agacggggct ccgcctggat cccgccgaga tccgcgactt
     1801 cgcggtgctg accgccgacg gctatctgct ggtcttcggt ctggctgtcc cgcacaccgc
     1861 cgccgatctg ccgcccttca ctccgaccaa cgagaccagc gagcgcgtcg tgctgcccgg
     1921 ccccaccgac ctggccttct cgctgcatac gcaggcgctg gcgatgtact tcgcggcccg
     1981 cgccggggtc ggtccggtgc cgcccgccgc gggcagccgc gcgtagcggg tgcgctgcta
     2041 gcacaccccc ggcgggaggg gcgagtggtg caggcccgcg ctttccagtg cctggacggc
     2101 ggcgcagggg tcgaaggctt gcgccggccc cagcaccccg cgggcggtga atcccggttc
     2161 cagcgtttgc agcgcgcacc agcagacgat ggccgcggcc aggcgataga cctgccggcc
     2221 catcaggatc acgttgcgcc ggcccttgcc gctgcgcgcc gtcgccttga cgacgcagat
     2281 cgattgttcc acgaagtcgg gaggcgggtt gcgggtaatc ggttcgatga tccggccgat
     2341 gagcgggctg actttgggca gcgccgggcc gagcgcgccc aggaacagcg cgacgggcgt
     2401 gggcaacgcc aggtagctgt ccatgctcgt cacctggata tggcggggga tctggatcag
     2461 ttcgcccagg ggggagttga gggccagccg ccggccaaac ggcgcgggta gccgcacgcg
     2521 gcgcatctgc cgggcgggcg gatagggcac ctgcttgccg ccgcgccagc cctgggtggg
     2581 ctgggccagc gcgaggaaga acgaccgctg cgtgcccttg ctgggccggt cgcgggtgat
     2641 gccgaacgtg gtggcgatgt ggtccaccgg ttccaggccc cgcgccgcca gcgccaccgc
     2701 ccagttggac agggcgtatt cggtgccgca agtcgggacg accacgcagc cccgttcgcg
     2761 cgccgccgca tcatagcgtt caatgatctg ccggatgaag ggcggttccc cgctcaggtc
     2821 gaggtaatgg actcccgccg ccaccgccgc cgctacgacc ggctcgccca ggcgcaaaaa
     2881 cggccccacg cagttgatca ccgtgcgcac gccgtcctcg aacatgggcg gcagcgtctc
     2941 gggatggtcg acatcggcga catggcgggg cacgtctgga tcgagtccga actccgcgat
     3001 cagcgcctcc atgcgcgcgg cctggcggtc ggccaggacc atcggctggc cccggcgggc
     3061 caattcctcg accaccatgc gcccgctgta ccccccggcc ccaaacacca gaatccgatc
     3121 gcccatcccg ccgccctcct ctgcctgatg gcccaatctg atgccgggcg tgccgctccc
     3181 ccggtggtgc cgatcctagc atccgctgcg tggcgtgtca agcacgtaca atttgctgtt
     3241 gctgtcaggg ccgatccatc ttgcgatacc gcctgagatt gggctataat gaggtttaac
     3301 ggctgctgat cgcgcctgat ctgcgcggca tccggcgacg tgtttgtgct ttgtaggcag
     3361 gaggaagacg ccatgtgtcg ctacgcagaa catgtctaca aaatgccgtt tgcctgcttt
     3421 gcgtgccgca aggtgttcaa gcagccgagc tactgggatc tttccccggc gcagcggccc
     3481 gcgcccggcc agccgcggac gatcccctgc ccgcaatgcc gccgcccgat gcacgccatg
     3541 ggccatgact tccgggcgcc caggcagcgc gacatcgcgc aatgggcgaa agtcgcggtc
     3601 ctgttccggc acggcatcac ataccattcc tgcggttgca acggccccgg cccgcgcccg
     3661 gccacgctcc gcgaggtacc cgagtttctc gcggaccacc tgccgcagtc gcccggcgcc
     3721 cggctcctgg cgcaatggca accatccgcc gaacgcccgc aacgccggtc gcgtggtggc
     3781 ggatcgcggc tgcacgcacg ctaaccagta cacgggctaa gccggatagc gggcgcggat
     3841 ggcggcgagg gccgtggcgg cggcgctgcg aaggcgcgcc tggcggcgcc cggtggagtc
     3901 ggcggtaatg gtcgccgggt cggtttgctg gatgcgctcc agggccggga tgctctcagg
     3961 ggtgccggcg gcacccaggg cttgcacggc ggcatagcgt acgtaggcgt tgccgtcgtc
     4021 caactggccg atcaggatcg gcgtggcggt ggcgtctcca atcgtgccca cgacctcgat
     4081 tgcctgcaca tgcagcatgg tgggcgcggc tggggtcatc cctgcgctaa cgccgccgct
     4141 ttcctggatc acgcgcaggg cgaccggcac ggcccgcgtg tcgccgagcc gggccagggc
     4201 cttcagcgct tccccgcgca ccaggggcgg ctgcgcggcg tcctccgcca cgcgcagcag
     4261 gggatcgcag gcctgggcat cgtccagctc ccccagccgg tgcgccgcta cccattgccg
     4321 gt
//
LOCUS       DASUYC010000357         3769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1796880, whole genome shotgun sequence.
ACCESSION   DASUYC010000357 DASUYC010000000
VERSION     DASUYC010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3769
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1796880"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(254..1846)
                     /locus_tag="VF276_05895"
     CDS             complement(254..1846)
                     /locus_tag="VF276_05895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX6606433.1"
                     /translation="MNRATRTDRARPWPARGDWAGWLLLAALVLLGALLVLKVIDTIQ
                     IMIGLFNWPFQSDESESMIVAETLLLDRGVDIFGPITPQQFIAAPYPPLYYLLNWPFI
                     HVLGPTFKPGRAISLLATAGVGLGIYAITWRLTHDRLAGLFGALGWGAIGIVGFWGAL
                     VKPDMFAVACGLGGLWWVLRTSEDGRGAVWWALPFFWAAFYSKQTAIAAAVAACVWLI
                     LRHWRTGLLFTGLYALGTAGGYLLLDWLTAGGFFYHEFTIHDLPWFAGRFVDTLTAWL
                     ETYWLLVGVGLLGVLAVFARDAGALLREAGVSGLLRRLGRWDTLAAPQGGVLLLGYAG
                     MGVFAASGVATLGGNHNHLLDLTAAGCLGFALALALLRRAPGRVPRAVGALVVLGLCT
                     QVPLLYDTPHWLGHELRVPPQSVREGMRNIAQYTSNTPGPVYSTNLSVLLATDKWRLN
                     LWTTDPFTQTHATLLGRWDESALVQAIREGRFALIILDINLDNPTDAAGNLSPGLQAA
                     IRAAYHLDQRNVLNLYKPNGAR"
     gene            complement(1843..>3769)
                     /locus_tag="VF276_05900"
     CDS             complement(1843..>3769)
                     /locus_tag="VF276_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606434.1"
                     /translation="LAWLAGRAAARAPALGLSRRAAERTAALIGAVWLPFTLFANLLL
                     SETLFLLLMVGAFVLLARYADARLPVAGAAPQEADRARSTRTLLGAGLLLGAAALTRS
                     TALGFIPLAVLWAGWVAWRAAPGRGIPWRAPALVLLGALVLLVPQVVRNYVAYHGLIL
                     GDTSSSFNLWLASVGVRDGARLAGDLAAIPSPLDKQSYAYSQAFANITVQPLAFLAKG
                     VRESRDLWTINFTAEERQTRGYTLGRVPAVHLWSLLAGEDLLYVGIVLLALLGLATGP
                     PDPFKALAGLWTLLWMAMAFVFFAVTRFRFPVVAMLLPWVPLGGAYLRTLLPARIRAQ
                     AAGPVPRPSRRAAGLYALGLLFAILVVPTIPLSETATGAERWAAQAPYRAAVPLVTGR
                     PAAALDLLARAAPDAPDTLYATDAAQLAQEATADTPNPAVLDRLANRPEVSLGPGSPL
                     GAGYEPYLLSGAIARLGGDPAGARSAFRARAVQTAGAEAVDWAAQFLLPPRTNRIDLG
                     GDLDFGAPQGFYNSERSGSGDAAITYRWSGAASLIRLRPATPWSTLVIRWSGARPPGV
                     PPAQVTATLRVPGGDTLRTVTLPAVDTWVETVLRPGSATPVTSPRVELRLAANAFVPG
                     GYDPRTLGVRVDWVELR"
BASE COUNT          607 a         1358 c         1356 g          448 t
ORIGIN      
        1 ggctgtggaa atgtgggcag ctctacggac ctgcccacat ttccacccgc cctgctgcgg
       61 accgctccgt tggcactagg tcaggatgta tcctagaggt tgcacaacgt cgaaaggaga
      121 ccgaccatga cccagcacga acattatact ctgtccgccg actggaaagt gaccttcgag
      181 tacgagccgt agattgtcat aagcactgtc ttttcatcat gagaacatgt aactgaacaa
      241 caaccgcccc gccctaccgc gctccattcg gcttgtagag gttgagcacg ttgcgctggt
      301 cgaggtggta ggcggcgcgg atggcggcct ggaggcccgg cgagaggttg cccgccgcgt
      361 cggtcgggtt gtccaggttg atgtcgagga tgatcagggc gaagcggccc tcgcggatgg
      421 cctgcaccag cgccgactcg tcccagcggc ccagcagcgt ggcgtgggtc tgggtgaagg
      481 ggtcggtcgt ccagaggttc agccgccact tatcggtcgc cagtagcacg ctcaggttgg
      541 tggagtagac cgggccgggc gtgttgctgg tgtactgggc gatgttgcgc atgccctcgc
      601 ggacgctctg cggcggcacg cgcagttcgt ggcccagcca gtgcggcgtg tcgtagagca
      661 gcggcacctg cgtgcagagg cccagcacaa ccagcgcgcc cacggcccgc ggcacccggc
      721 ccggcgcccg gcgcagcagc gccagggcca gcgcaaagcc caggcacccg gcggcggtca
      781 ggtcgagcag atggttgtgg ttgccgccca gggtcgccac gccgctcgcc gcgaacacgc
      841 ccatgccggc gtaccctagc agcagcacgc cgccctgcgg cgcggccagc gtgtcccagc
      901 ggcccaggcg gcgcagcagg ccggacacgc ccgcctcgcg cagcagcgcc ccggcgtcgc
      961 gggcaaagac cgccagcacg ccgagcagcc ccacgcccac cagcagccag tacgtctcca
     1021 gccacgcggt cagcgtgtcc acgaagcggc ccgcgaacca gggcagatcg tggatggtga
     1081 actcgtggta gaagaagccg cccgccgtga gccaatcgag cagcaggtag ccgcccgccg
     1141 tgcccagcgc gtagaggccg gtgaacagca gcccggtgcg ccagtggcgc aggatcagcc
     1201 agacacaggc ggcgacggcg gcggcaatcg ccgtctgctt gctgtagaag gctgcccaga
     1261 agaagggcaa cgcccaccag acggcgcccc ggccgtcttc gctcgttcgc aggacccacc
     1321 acagtccgcc caggccgcag gccaccgcga acatatcggg cttgaccagt gcgccccaga
     1381 agccgacgat gccgatggcg ccccagccca gcgcgccgaa cagcccggcc agccggtcgt
     1441 gcgtcagccg ccaggtgatg gcgtagatgc cgaggcccac gcccgccgtc gccaacagcg
     1501 agatggcgcg gcccggcttg aatgtcggcc ccaggacgtg gatgaacggc cagttcagca
     1561 ggtaatagag cggcggatag ggcgcggcga tgaactgctg cggcgtgatc gggccgaaga
     1621 tgtccacccc gcggtcgagc agcagcgtct cggccacgat catgctctcc gactcgtcgg
     1681 actggaaggg ccagttgaac agcccgatca tgatctggat ggtatcgatc accttgagca
     1741 cgagcaacgc gccgagcagc accagggccg ccagcagcag ccacccggcc cagtcgcccc
     1801 gcgcgggcca gggccgggcg cggtccgtgc gggttgcgcg attcatcgca gttccaccca
     1861 atcgacgcgc acacccagcg tccgcggatc gtagccgccg ggcacaaagg cgttggccgc
     1921 caggcgcaac tcgactctgg ggctggtgac cggcgtggcg ctgccggggc gcagcacagt
     1981 ctccacccag gtatcgacgg ccggcagcgt gaccgtgcgc agggtgtcgc cgccggggac
     2041 gcgcagcgtg gccgtgacct gggcgggcgg cacaccgggc gggcgggcgc cgctccagcg
     2101 gatcacgagg gtgctccagg gagtcgccgg gcgcaggcgg atcaggcttg cggcgccgct
     2161 ccagcggtag gtgatcgccg cgtctccgct gccgctgcgc tcgctgttgt aaaagccctg
     2221 cggcgcgcca aagtcgagat cgccgccgag gtcgatgcgg ttggtgcgcg gcggcagcaa
     2281 gaactgcgcg gcccaatcga ccgcctccgc ccccgccgtc tgcacggcgc gcgcccggaa
     2341 cgccgaccgc gcgcccgccg gatcgccgcc gaggcgggca atcgcgccgc tgagcaggta
     2401 cggctcgtag cccgcgccca gcgggctgcc ggggccgagc gacacttcgg gccggttggc
     2461 gagccggtcg agcacggcgg ggttgggcgt gtccgctgtc gcctcctggg ccagttgggc
     2521 ggcatcggtg gcgtagagcg tgtcgggcgc gtcgggcgcg gcgcgggcca gcaggtcgag
     2581 cgccgccgcg ggccggcccg tcaccagcgg cacggcggcc cggtagggcg cctgggccgc
     2641 ccagcgctcc gccccggtgg ccgtctcgct cagggggatc gtgggcacga ccaggatggc
     2701 aaacagcagg ccgagcgcgt agagccccgc cgcgcgccgg ctgggccgcg gcaccggccc
     2761 cgccgcctgg gcgcggatgc gcgcgggcaa gagcgtgcgc aggtacgcgc cgcccagcgg
     2821 cacccacggc agcagcatcg ccaccaccgg gaagcggaag cgcgtcacgg cgaaaaagac
     2881 gaaggccatc gccatccaga gcagcgtcca gaggccggcc agcgccttga acgggtcggg
     2941 cggccccgtc gccaggccca gcagcgccag cagcacgatc cccacgtaaa gcaggtcttc
     3001 gcccgccagc aggctccaga ggtgcacggc gggcacccgg cccagcgtat agccccgcgt
     3061 ctggcgctcc tcggcggtga agttgatcgt ccagaggtcg cgcgattcgc gcacgccctt
     3121 cgccaggaaa gcgagcggct gcaccgtgat gttggcgaac gcctggctat aggcgtagct
     3181 ctgcttgtcc agcgggctgg ggatggcggc caggtcgccg gccagccgcg cgccgtcgcg
     3241 cacgccgacg ctcgccagcc agaggttgaa gctgctgctg gtgtcgccca ggatcaggcc
     3301 atgataggcg acgtagttgc gcactacctg cggcaccagc agcaccagcg ctcccagcag
     3361 caccagcgcg ggcgcccgcc aggggattcc ccgccccggc gccgcccgcc acgccaccca
     3421 ccccgcccag agcacggcca gggggatgaa ccccagcgcg gtcgagcggg tcagcgccgc
     3481 cgcgccgagc aacaggcccg cgccgagcaa cgtccgcgtg ctccgcgccc ggtccgcctc
     3541 ctgcggcgcc gcgcccgcga ccggcaggcg cgcatcggcg tagcgcgcca gcagcacgaa
     3601 cgcgcccacc atcagcagca ggaacagcgt ctcgctgagc aagaggttgg cgaacagggt
     3661 gaacggcagc cagaccgcgc cgatcaatgc ggcggtgcgt tcggcggcgc ggcgcgagag
     3721 gccgagcgcc ggggcgcgcg ccgccgcccg cccggctagc caggccagc
//
LOCUS       DASUYC010000358         2735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_967060,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000358 DASUYC010000000
VERSION     DASUYC010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967060"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..1047
                     /locus_tag="VF276_05905"
     CDS             37..1047
                     /locus_tag="VF276_05905"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEX6606435.1"
                     /translation="MEQNSVIPAAPATASAEAAPPAGPAWMQRARALLGNKRLRRVLQ
                     IAVIGVVFFFLARAVWLEWPDIQAYPWHLEIGYVLAALLVLLVRGPIICLGWRMILRF
                     MGYPLPWATAIRVYFYSGLAKYMPGSMWYAVGRVLLAEQAGVPKMITSVSIALETALV
                     TVASITVGALALTARPDTPWWPLALVLAGLLVFLAYPRPWFALMNRGLQLLGRQPVTI
                     TITGRQLLLLLPPFILSWVAYGFISFFWTAALYPQLPWSEWPAITGLYTAAWVIGFLT
                     LIVPNGWGVREGLLIGFLVNLLHLNPAVALGAVLLSRLGSIFGESAWAAIAWSIKPPR
                     QS"
     gene            1061..2320
                     /locus_tag="VF276_05910"
     CDS             1061..2320
                     /locus_tag="VF276_05910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6606436.1"
                     /translation="MRILHLIYDDIGNPWLGGGGAVRTLEINRRLAARGHQVIVGCGA
                     YPAAPPSAPRDGVLYLRVGARGWGGPPRSYLSSRLRYAWGAGALLKDLGYDIVVEDFS
                     PYSPVFAPWRARRGAAVVASVQNLSGAHAVRKYGRGPRGLLPRLLERPLLRRFSNVVT
                     VSQGIADEMRVWWRPGRGGRIAVIPNSAGPAFAAVTGTPAPPEAPVILFLGRIDPYQK
                     GLDTLLRAYALAAPRMPGVRLELAGTGTPEAVQQVRAWAAEQRLPVIDTDSDAVPAEA
                     PGGPLVVWRGKLEGADAVAALRRCLFLAMPSRYEAWPITALEAAVCERPVLGTDVTGV
                     RDAAPATAHGRLVPPDDPAAFAEAMVALAGDAGLRHALGARGQAWAAAFTWDRLAAQQ
                     ERFYQEVLAATAPSPAATREDRDTGPA"
     gene            2317..>2735
                     /locus_tag="VF276_05915"
     CDS             2317..>2735
                     /locus_tag="VF276_05915"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6606437.1"
                     /translation="MILFITRKHPPSIGGMQKLSQSLTVEMSKLAPTTTISWGRSTIF
                     LPFFLFYALLRALVIIPWNRRIQVIHLSDALLAPLGLLLKTLFRVPVVVTVHGLDITF
                     DNGFYQWLVPRCLVHMDKLICVSGYTRDQCLRRGIPAA"
BASE COUNT          346 a          917 c          987 g          485 t
ORIGIN      
        1 cataagcgga gcctgataac gtggatcgga aaccggatgg agcagaatag cgtgataccg
       61 gcagccccgg cgacggcgtc cgcggaggcg gcaccgccgg cgggcccggc ctggatgcag
      121 cgggcgcgcg cgctgctggg caacaagcgg ctgcgccggg tgctccagat cgccgtgatc
      181 ggcgtggtct tcttcttcct ggcccgcgcg gtctggctgg agtggcccga catccaggcg
      241 tacccctggc atctggagat cggctacgtg ctggcggccc tgctggtctt gctggtgcgc
      301 gggccgatca tctgcctggg ctggcgcatg atcctgcgct tcatgggcta cccgctgccc
      361 tgggccacgg cgatccgcgt ctatttctat tcgggcctcg ccaagtacat gcccggctcg
      421 atgtggtatg ccgtgggccg cgtgctgctg gccgagcagg cgggcgtgcc gaagatgatc
      481 acctcggtga gcatcgcgct ggaaaccgcg ctggtgacgg tggcctcgat cacggtcggg
      541 gcgctggcgc tgacggcgcg gcccgacacc ccgtggtggc cgctggccct ggtgctggcg
      601 gggctgctgg tcttcctggc ctatccgcgg ccctggttcg cgctgatgaa ccgcgggctg
      661 caactgctgg gccgccagcc ggtgacgatc accatcacgg gccggcagtt gctgctgctg
      721 ttgccgccgt ttatcttaag ctgggtggcc tacggcttca tctctttctt ctggactgcc
      781 gcgctctatc cgcaactgcc ctggagcgaa tggccggcga tcaccgggct gtatacggcg
      841 gcctgggtga tcggcttcct gacgctgatc gtgcccaacg gctggggcgt gcgcgagggc
      901 ttgctgatcg gcttcctggt caacctgctg cacctcaacc cggcggtcgc gctcggcgcc
      961 gtgctgctgt cgcggctcgg ctcgatcttc ggcgagtcgg cctgggccgc catcgcgtgg
     1021 agcatcaagc ccccccggca gtcctgaacc tcgttgccgc atgcgtattt tgcacctgat
     1081 ttacgacgat atcggcaatc cctggctagg cggtgggggc gcggtgcgca cgctggagat
     1141 caaccgccgg ctggcggcgc gcggccacca ggtgatcgtg ggctgcgggg cctatccagc
     1201 cgcgccaccc tcggcgccgc gcgacggcgt gctttatctg cgggtgggcg cgcgggggtg
     1261 gggcgggccg ccgcgcagct acctgagcag ccggctgcgc tatgcctggg gcgcaggcgc
     1321 cttgctgaaa gatctgggct atgatatagt ggtcgaagat ttttcgccgt atagccctgt
     1381 cttcgcgccg tggcgcgccc ggcgtggcgc ggctgtggtt gccagtgtgc agaacctgtc
     1441 cggcgcccac gccgtccgca agtatggccg ggggccgcgc ggcttactgc cgcgcctgct
     1501 ggagcggccc ctgctgcgcc ggttttccaa cgtggtgacg gtgtcgcagg gcatcgccga
     1561 tgagatgcgg gtctggtggc gaccgggccg gggcgggcgg atcgcagtga tcccgaacag
     1621 cgccggcccg gcctttgccg ccgtgaccgg gacgcccgcg ccgccggaag cgccggtgat
     1681 tttgtttctg ggccgcatcg atccgtacca gaagggcctg gacacgctgt tgcgggcgta
     1741 tgcgctggcc gcgccacgga tgccgggggt gcggctggaa ctcgccggca ccggcacgcc
     1801 ggaagcggtg cagcaggtgc gtgcctgggc cgccgagcag cgcctgccgg tgatcgacac
     1861 ggactcggat gcggtgcccg ccgaggcgcc cggcgggccg ctggtggtct ggcggggcaa
     1921 gctcgaagga gcggacgccg tggccgcgct gcggcgctgc ctgttcctgg cgatgccgtc
     1981 gcgctacgaa gcctggccga tcacggcgct ggaagccgcg gtgtgcgagc gcccggtgct
     2041 gggcacggac gtgaccgggg tgcgtgatgc ggcgcccgcc acggcgcacg gccggttggt
     2101 cccgccggac gaccccgccg cgttcgccga ggcgatggtc gcgctggccg gcgatgccgg
     2161 actacgccac gcgctgggcg cgcggggcca ggcctgggct gcggccttta cctgggaccg
     2221 cctggcggcg cagcaggaac gcttttacca ggaagtgctc gccgccacgg cgccgtcgcc
     2281 cgccgccacg cgggaggacc gagataccgg gccagcatga tcttatttat cacacgcaaa
     2341 cacccgcctt ctatcggggg catgcaaaaa ctcagccaga gcctcaccgt ggagatgagc
     2401 aaactggcgc cgaccaccac gatctcgtgg ggccgttcga cgattttcct gccgtttttc
     2461 ctgttctatg ccctgctgcg ggcactggtc attatcccct ggaatcggcg catccaggtg
     2521 atccacctga gcgacgcgct gctggcgccg ctgggcctct tgctcaagac gctgttccgc
     2581 gtgccggtgg ttgtcaccgt ccacggcctc gacatcacct tcgacaacgg cttctatcag
     2641 tggctggtgc cgcgctgcct ggtccatatg gacaagctga tctgcgtcag cggctacaca
     2701 cgcgaccaat gcttgcgccg gggcatcccc gccgc
//
LOCUS       DASUYC010000359         4723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_499310,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000359 DASUYC010000000
VERSION     DASUYC010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499310"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..1045
                     /locus_tag="VF276_05920"
     CDS             230..1045
                     /locus_tag="VF276_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6606438.1"
                     /translation="MKFMRILAVDDDESTLAFLRVRLEQDGFEVSVARTGPEALELAA
                     TLMPDLALVDLRLPGMDGFELSRRLLQISEIPIIMLTAVADEQTVVEGLERYAEDYVN
                     KPFHYPELRARITRVLSRRTPDPTTGRMVIDEHLTIDLPRRTVWVDGTPQRLSPIETR
                     LLATLVQNRGRVVPQDALLRRAWGHLNEGEPDSLWVRMRNLRRKIGDAANPPRYVLTE
                     RGVGYRFAEPEPEAPAEPPSGNGRADSAAMRRTTHSAATLPVVTKVVAGLPTG"
     gene            1042..2337
                     /locus_tag="VF276_05925"
     CDS             1042..2337
                     /locus_tag="VF276_05925"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEX6606439.1"
                     /translation="MSLAQGMERAEPPAQAVPAGGEAGTGRPPARILVVDDNPDGMAL
                     LVALLQAQGYAVETATTGQAALSSVAANPPALMLLDVMLPDLDGHEVARRVKHDTNLP
                     FIPVILVTAKSELRDKIYGLEQGADDFLSKPVNSAELMARVHALLRLKRAQDDLRQQH
                     EQLRTLHAQLRDSEATREELVQMITHDLRGPLTGLLGALELIEDGSLGPLTSDQRQFI
                     DQSLRNCQILNEMVTDILDVNRLEAGHTELDYEPVDLRALADLACTQVQAAVAGKKLE
                     LVNDIAPDLPPAWADRGKLVRVLANLLNNAFKYTERGTITVRAGVVGDNGAAHAAPGE
                     GQPTDAARQYLRVEVWDTGVGIPDEARPQVFQKFFRVRNRRAAGVARGTGLGLYFCKH
                     VVEAHGGNIWVDARPDGAPGSVFAFTVPLAPEPHASQAR"
     gene            2337..2708
                     /locus_tag="VF276_05930"
     CDS             2337..2708
                     /locus_tag="VF276_05930"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6606440.1"
                     /translation="MARILIVEDEPLSRQVLQRRLSKAGYDVDSADNGRMALQTLTAA
                     DTLPDVILTDLHMPEVSGYDLCASVRRNPRLAHLPIIIMSALDGAEYRESIQSFNVAR
                     YLTKPLAMDEVFAAIAAALAS"
     gene            2760..3173
                     /locus_tag="VF276_05935"
     CDS             2760..3173
                     /locus_tag="VF276_05935"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6606441.1"
                     /translation="MPYTLLVVEASETNRDMLATLLRRNGYRVLEAGNGAEALTMAAQ
                     EPIDLVLLDLSLTDLDDGEILRRMQALPALAGVPIIGVSTHNRPGAAEVARAAACAGY
                     ITRPIALASFLDLLAHHLRQAPPGPPDTGPDSLPT"
     gene            complement(3276..4595)
                     /locus_tag="VF276_05940"
     CDS             complement(3276..4595)
                     /locus_tag="VF276_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606442.1"
                     /translation="MSEHARDLLLRGRDAVLAKEPEEARFYLEWVLSTDSDIDQQTEA
                     WSLLSQVAATPAEQRSCLEEVLAAYPGHAAARRDLAVLDGRLKPEDILDPWAPVSPIQ
                     PPATPAPSEVQRYLCPSCGGTLAHNPRRGVLACVFCGYQRPAPAATGNGAVEERDWIA
                     TMHTARGHRWVLPTARTLGCQGCGATVLLPPAHAGAACAFCGAPYVVVIVHQDLVEPE
                     GIIPFAVDGPTALARAQAWIKARRFRRDDLATATFEVPRPIYLPFWTFDLYGTVVAHR
                     PPPATRFGNPVPAPPPARYTVFCDDLLVPATTGLPAPLLEALHFDTQALVPYTAEHLV
                     TWPAEIYRVAMADASVQAHSRVIPRFEKEALRIDVALGDSEVTLTVDRAGTYVSSYKH
                     VLLPVWVTGYAHKGERYPVIVNGQSGQVHGRLPQGPLGRFLSGLVEP"
BASE COUNT          783 a         1566 c         1653 g          721 t
ORIGIN      
        1 ccttaccggc ataggaaatt caggctattt tggtaagggt tcgacacttg gtaaggattt
       61 tggtaagcta atgctgatat agtcatggta gcaaacctta ccaaatgcac aacggaacgg
      121 cgagatggga gccaggcgtg cggtagggtg cggatgcatg gaacgaggag gcccccggag
      181 tcgtgaggct gcacgactcc ggcctggaga aaggaactag cacaccgcga tgaagttcat
      241 gcgtattctg gcggtggacg acgatgagtc cactttagcc tttctgcgag ttcggttgga
      301 acaggatggc tttgaggtat ccgtagcccg caccgggccg gaggcgctgg agctggccgc
      361 cacgctgatg ccggacctgg ccctggtcga tctgcggctg ccggggatgg atggcttcga
      421 gttgagccgg cgcttgctgc agatctcgga gatcccgatt attatgctga ccgcggtggc
      481 cgacgagcag accgtggtcg aggggctgga gcgctacgcg gaagattacg tcaacaagcc
      541 cttccactac ccggagctgc gcgcgcgcat tacgcgagtg ctctcgcggc gcacgcccga
      601 cccgaccacc gggcggatgg tgatcgacga gcacctgacc atcgatttgc cccggcgcac
      661 ggtctgggtg gacggcacgc cgcagcgcct ctcgccgatt gagacgcggc tgctggcgac
      721 gctggtgcag aaccgcgggc gcgtggtgcc gcaagacgcg ctgctgcggc gcgcgtgggg
      781 ccacctgaac gagggcgagc cggactcgct gtgggtgcgt atgcgcaacc tgcgccgcaa
      841 gatcggcgac gcggccaacc cgccgcgcta cgtgctgacc gagcggggcg tcggctatcg
      901 cttcgccgaa cccgaacccg aagccccggc agagccgccc tccggcaacg ggcgcgcgga
      961 cagcgccgcc atgcggcgca ccacgcactc ggcagctacc ctgcccgtgg tcaccaaagt
     1021 ggtcgccggg ttgccgaccg gatgagcctg gcgcaaggca tggagcgcgc agagccgccg
     1081 gcgcaggcgg tcccggccgg cggcgaggcc gggaccgggc gcccgcccgc ccgcatcctc
     1141 gtggtcgacg acaatcccga cggcatggcc ctgctggtgg ccttgctgca agcccagggc
     1201 tatgccgtcg agacggcgac gaccgggcag gcggcgctga gcagcgtggc ggccaatccc
     1261 ccggcgctga tgttgctcga cgtgatgctg cccgacctcg atgggcacga agtggcccgc
     1321 cgggttaagc acgacaccaa cctgccgttc atcccggtca tcctggtgac ggcgaaaagc
     1381 gaactgcgcg ataaaatcta cgggctggag cagggcgccg acgatttcct gagcaagccg
     1441 gtcaacagcg ccgaactgat ggcgcgggtc cacgccctgc tgcggctcaa gcgcgcccag
     1501 gacgacctgc gccagcagca cgaacagttg cgtaccctgc acgcgcagtt gcgcgactcc
     1561 gaggccacac gcgaggaact ggtgcagatg atcacccacg atctccgcgg cccgctgacc
     1621 gggctgctcg gcgcgctcga actcatcgag gacggcagcc tggggccgct cacgtccgat
     1681 cagcgccagt ttatcgacca gtcgctgcgc aactgtcaga tcctcaacga aatggtcacc
     1741 gatatcctcg acgtgaaccg gctggaggcc gggcacaccg aactcgatta cgagccagtg
     1801 gacctgcgcg cgctggcgga cctggcctgc acccaggtcc aggcggcggt tgccgggaag
     1861 aagctggaac tggtcaacga tatcgcgccc gatctgccgc ccgcctgggc cgaccggggc
     1921 aagctggtgc gggtgctggc gaacctgttg aacaatgcgt tcaagtatac cgagcgcggc
     1981 acgatcacgg tgcgcgccgg tgtggtaggc gataacgggg cggcgcacgc cgcgcccggc
     2041 gaggggcagc ccacggatgc cgcgcggcag tacctgcggg tcgaagtgtg ggacacgggc
     2101 gtgggcatcc ccgacgaagc gcgcccgcag gtcttccaga agttttttcg cgtgcgcaac
     2161 cggcgcgccg ccggggtggc gcgcggcacc gggctgggtc tctacttttg caaacacgtg
     2221 gtggaagccc acggcggcaa catctgggtg gacgcccgcc ccgatggcgc gccgggcagc
     2281 gtgtttgcgt tcaccgtgcc gctcgcgccc gagccgcacg cctcgcaggc ccgctgatgg
     2341 cccgcatcct cattgtcgaa gacgagccgc tgtcgcggca ggtgctgcaa cgccggctga
     2401 gcaaagccgg gtacgacgtg gattccgccg acaacgggcg catggcgctg caaaccctga
     2461 ccgccgccga caccctgccc gacgtgatcc tgaccgacct ccatatgcct gaagtgagcg
     2521 gctacgacct ctgcgccagc gtgcgccgta acccccgcct cgcccatctc cctatcatta
     2581 ttatgagcgc cctggacggt gccgaatatc gagaatccat ccagtcgttt aacgtggcac
     2641 ggtatttgac caagcccctg gcgatggatg aagtatttgc cgcgatcgcg gcggcgctgg
     2701 cgagctgacg gggcacccgc cgcgcccgag gtggcggccc gcgggccgca aggtgaagta
     2761 tgccgtatac actgctcgtg gtagaagcga gcgaaaccaa ccgcgatatg ctggcgacgc
     2821 tgctgcgccg caacggctat cgcgtgctcg aagccggcaa cggcgcggaa gcgctgacca
     2881 tggccgctca ggaaccaatc gatctggttc tcctggattt gtctctcacc gacctggacg
     2941 acggcgagat cctgcgccgg atgcaggcgc taccggcgct ggccggcgtg ccgatcatcg
     3001 gcgtcagcac ccacaaccgg cccggcgccg ccgaggtcgc ccgcgccgcc gcgtgcgcgg
     3061 gctacattac ccggcccatc gccctggcct ctttcctcga tctgcttgcc catcacctgc
     3121 ggcaggcacc ccccggcccg ccggacaccg gccccgattc gttgccgacg taattaaccg
     3181 cgaaggcgcg aaggacgcga agacgatttt tattaatcgt attcttcgcg tccttcgcgc
     3241 cttcgcggtg cgtttatgaa cggcgggggc gggggctacg gctcgaccag accgctgagg
     3301 aaccggccca gcggcccctg tggcaaccgg ccatgcacct gcccgctctg cccgttgacg
     3361 atgaccgggt agcgctcccc tttgtgcgca tagcccgtga cccagaccgg cagcagcacg
     3421 tgcttgtagg aactgacgta ggtgcccgcc cgatccaccg tcagggtcac ctcggagtcg
     3481 cccagggcca cgtcgatgcg cagggcctct ttctcgaagc gggggatgac gcggctgtgc
     3541 gcctgcaccg aggcatcggc catggcgacg cggtagatct cggcgggcca tgtgaccaag
     3601 tgctcggcgg tataaggcac cagcgcctgg gtgtcgaagt gcagcgcttc cagcagcggc
     3661 gcgggcagcc cggtcgtagc ggggaccagc agatcgtcgc agaacaccgt atagcgggcg
     3721 ggcggcggcg ccgggacagg gttgccgaag cgggtggccg gcggcgggcg atgggccacc
     3781 acggtaccgt agaggtcgaa ggtccagaac ggcaggtaga tgggccgcgg cacctcaaag
     3841 gtggccgtgg cgagatcgtc gcggcggaag cggcgcgcct tgatccacgc ctgcgcgcgc
     3901 gccagggccg tggggccgtc gacggcgaag gggatgatgc cttcgggttc gaccaggtcc
     3961 tggtggacga tgaccacgac atagggcgca ccgcagaagg cacaggcggc gccggcatgg
     4021 gcggggggca gcagcaccgt ggccccgcac ccctggcatc ccagcgtgcg cgcggtgggc
     4081 aacacccagc ggtggccgcg cgccgtgtgc atggtggcga tccagtcgcg ctcttccacg
     4141 gcgccgttgc cggtggccgc gggggcgggg cgctgatagc cacagaacac acaggccagc
     4201 acgccgcgtc gcgggttgtg ggccagggtg cccccgcaac tggggcagag gtagcgctgg
     4261 acctcgcttg gcgcgggcgt ggcgggcggc tggatgggcg acaccggcgc ccagggatcg
     4321 aggatgtctt ccggcttgag gcgcccgtcg agcacggcga ggtcccgccg ggctgcggcg
     4381 tggccggggt acgcggccag cacttcttcc aggcaggacc gctgctcggc gggtgtggcc
     4441 gcgacctggc tcagcagaga ccaggcttcg gtctgctggt caatatccga gtcggtggac
     4501 agcacccatt cgagatagaa gcgcgcttcc tcgggttcct tcgccaggac cgcgtcgcgc
     4561 ccgcgcaaaa gtagatcgcg cgcgtgttcc gacatcgccg cctccttatg cgccggcccg
     4621 ctgcgccgcg cgggcgctta gtgcagattc cagtgacctg ttgggtatag cggcgcgatt
     4681 tatcgcgccc gtactcgttt gtcgtcagca tgggcctgac cca
//
LOCUS       DASUYC010000360         2091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_531993,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000360 DASUYC010000000
VERSION     DASUYC010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531993"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VF276_05945"
     CDS             <1..451
                     /locus_tag="VF276_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606443.1"
                     /translation="DDTGRIVVAGLLSGGAFLAEQIVDQAIWPEGYSDMKLLGMAVTR
                     RSPHYWFVGVPWHLLNSIAFAFVYARVVGPRLPGPGWLRGLLMGLIENNVLWFTLLSV
                     GNRLHPAVRDGSMPPIRRLGVDWLVGNLRHIALGLVLGALCPVRGRE"
     gene            complement(787..>2091)
                     /locus_tag="VF276_05950"
     CDS             complement(787..>2091)
                     /locus_tag="VF276_05950"
                     /inference="COORDINATES: protein motif:HMM:NF015922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HEX6606444.1"
                     /translation="ILDMRLARLAAMERKKVEDELAEVIKQINYLEDLLAHPAKIYIL
                     IKQDLATLKEKYGDVRRTRILGDASGEFNEDDLIPEIRVLVTLTDKGYIKRLPHDTYK
                     RQNRGGRGVTGIVTREMDAVQHMITCNTLDNLLFLTNKGRIFTLRAHEVPDAGRQAKG
                     LPLVNLINLQPNELVTALVSVPDFNAAKYLVMATKQGKIKRTPLEAYSRVLSSGIIAL
                     SLEDGDELAGARLTHGDGTLIMVTRHGQGIRFKESDVRPMGRDATGVNAIRLDPKDYV
                     VGFDVVVPECHLLVVTDKGVGKRTPLGEFPIQGRAGGGVRAIALTDRSGLVAVARVVH
                     PDDDLVVISNKGLVIRMFAVAVSELRRPAQGVSVMSMRDNDGVASLARIPANGDPADK
                     HGGDGNGARRLDEALGGPTAVLTVPKGKAGNGHSNNGDGEAE"
BASE COUNT          289 a          698 c          728 g          376 t
ORIGIN      
        1 ggatgatacg gggcgcatcg tcgtcgccgg gctgctgtcg ggcggcgcct tcctggccga
       61 gcagatcgtg gaccaggcca tctggcccga gggttacagc gatatgaagc tgctgggtat
      121 ggccgtgaca cggcgcagcc cgcactactg gttcgtaggc gtgccctggc acctgctcaa
      181 ctcgatcgcc ttcgccttcg tctacgcgcg ggtggtgggg ccgcgcctgc ccggtcccgg
      241 ctggctgcgc ggcctgctca tgggcctgat cgagaacaac gtgctctggt tcaccttgct
      301 gtcggtgggc aaccggctgc accccgcggt gcgcgacggt agcatgccgc cgatccgccg
      361 gctcggcgtc gattggctgg tgggcaacct gcgccacatc gcgctggggc tggtgctggg
      421 cgcgctctgc ccggtgcgcg ggcgggagta acggcgcggc aggccggttt gaaaccggcc
      481 cctacaccgc gatcagtgcg cggaccggtt tgaaaccagc ccctacaacg tcatcaggct
      541 gacgagccgg tttgaaaccg gcccctacaa cgcagaatgg aggcaaaggt aacagcgggg
      601 cgcgggccgg ttttgaaacc ggcccctaca ccgcaagcgg gacgggggtc gatgcgaaac
      661 cggcctctac accctaacga cgcagcgggc cggtttgaaa ccggccccta cagcgcaaga
      721 ggtggacata ggaaaaggca gcgcggtttt cgccgcgctg cctctgtgtt actggtggaa
      781 cggggcttac tcggcctcgc cgtcgccgtt gttgctgtgg ccgttgccgg ctttgccctt
      841 ggggaccgtg agcaccgccg tcgggccgcc cagcgcctcg tcgagtcgcc gcgcgccgtt
      901 gccgtcgccg ccgtgcttgt ccgccgggtc gccgttggcc gggatgcgcg ccaggctggc
      961 gacgccgtcg ttgtcacgca tgctcatcac cgagacgccc tgggccgggc ggcgcaactc
     1021 gctgaccgcc accgcgaaca tgcggatcac caggcccttg ttgctgatga ccaccaggtc
     1081 gtcgtcgggg tgaaccacgc gggccaccgc gaccaggccg ctgcggtcgg tgagggcgat
     1141 ggcccgcacg ccgccgccgg cgcggccctg gatcgggaac tcgcccagcg gggtgcgctt
     1201 gcccacgccc ttgtcggtca cgaccagcag gtggcactcg ggcaccacca cgtcgaagcc
     1261 gacgacatag tccttcgggt cgaggcggat ggcgttgacg ccggtcgcgt cgcggcccat
     1321 cgggcgcacg tccgactcct tgaaccggat gccctggccg tggcgggtca ccatgatcag
     1381 cgtgccgtcg ccgtgggtca ggcgcgcccc ggccagttcg tcgccgtctt cgaggctgag
     1441 cgcgatgatg ccgctcgaca gcacccggct gtaagcctcc agcggcgtgc gcttgatctt
     1501 gccctgcttg gtcgccatca ccaggtactt ggcggcgtta aagtcgggca cggagaccag
     1561 cgccgtcacc aattcgttcg gctgcaggtt gatcaggttg accagcggca gccccttggc
     1621 ctggcggccc gcgtcgggca cctcgtgggc gcgcagggtg aagatgcggc ccttgttggt
     1681 caggaacagc aggttgtcga gcgtgttgca ggtgatcatg tgctgcacgg cgtccatctc
     1741 gcgggtgacg atgcccgtca cgccgcgccc gccgcggttc tggcgcttgt aggtgtcgtg
     1801 cggcagccgc ttgatgtagc ccttgtcggt cagcgtgacc agcacgcgga tctcggggat
     1861 caggtcgtcc tcgttgaact cgccgctcgc atcgcccagg atgcgggtgc ggcgcacgtc
     1921 gccgtacttc tccttgagcg tcgccagatc ctgcttgatc aggatgtaga tcttggccgg
     1981 gtgggccagc aggtcttcca gatagttgat ctgcttgatc acttcggcca gttcgtcctc
     2041 gaccttcttg cgctccatgg ccgccagccg ggccaggcgc atgtcgagga t
//
LOCUS       DASUYC010000361         3512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1693183, whole genome shotgun sequence.
ACCESSION   DASUYC010000361 DASUYC010000000
VERSION     DASUYC010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1693183"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..1326)
                     /locus_tag="VF276_05955"
     CDS             complement(142..1326)
                     /locus_tag="VF276_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606445.1"
                     /translation="MQETTRPIAPNTALYSRQNTTIRASLIGLGAMVLLALAILFGSR
                     MLTFFDPALTGYAIASLFAAFGVAFHYGTWLMRPSTRTMWRRSWQFFLKPENARRYFW
                     VIPRAFISNLLFQRFISKRSTLRWIMHQCLFWGVILSFAVTFPLTFGWVHFEVVGGST
                     YRVYLLGFPTITMDSLGPVGFNTFHALDYFAVLVIIGVAIAYWRRWQDRAVKPQQRVL
                     FDLLPLHLLMAISITGIMLTIVDFFFAGWMHWPITLTHQFLVIMMILYLPFGKLFHII
                     ERPASLGVEVYYAVGTEQGMRNCARCGRPFGMQIQVNDVKQALNGVGYNFWLPQEQHH
                     WQDYCARCKRVIRAEAYTGLSRRGWVNPGHLQQPQYSLPTSPVPEVTGNTGQDVPAIP
                     SA"
     gene            1788..2939
                     /locus_tag="VF276_05960"
     CDS             1788..2939
                     /locus_tag="VF276_05960"
                     /inference="COORDINATES: protein motif:HMM:NF015552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606446.1"
                     /translation="MAQAVLESPFVQLLLLGVASAAVRYFLLRRGESATASGAIAMAI
                     GIFVLGALQHMPFPVTDLTQILTLTGLIVWAYIAASYVRSYFRGTFHNHTDDPVGCFA
                     VGTWVAGTAVLARLVVIVLPTWRPLGLALAVLTLVVWLWYLTLIVGRYRIILASPTRL
                     RTTGRILLATVSTQSLVLLIEQLFPGQIPGGLATGLILLGYGFYGLGFVLIVQRYLRQ
                     PGWRLAEDWDNTNCILHGAMSITVLAALESRAVPDALIIASWLWAAVLFVIVEAIEIA
                     RLVARVRAYGWRGGFAVYQVSQWARNFTFGMFYAATARLQGLGGTAGLGLAGLSGLQA
                     AIVAWGQYMVLILLVIEISLFMLHNAGPRLQKLFAPAPSMTGAVETPQH"
     gene            2963..>3512
                     /locus_tag="VF276_05965"
     CDS             2963..>3512
                     /locus_tag="VF276_05965"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6606447.1"
                     /translation="MQNSRQAYVNLGLATLAFAVAFIAWSLISPLASQIQKDYGLDNT
                     AISFLIAVPVILGSLLRIPMGILTDRFGGRTVFTGLLLFTLLPVAFLGIAGSFWTYVL
                     GAFFLGTAGASFAVGVPFVNRWFTPDKQGLALGIYGMGNIGTAVAAFSMPGLVGAFQG
                     RAGAFWFYLVPVLLMAVLFWLLA"
BASE COUNT          555 a         1095 c         1153 g          709 t
ORIGIN      
        1 cgtcgggatc agcttgatcg cgtcctcggt ggtcgaccag ccgctagtcg aggagtatat
       61 cggttggatg gcctcttccg ggttcgccac gccggttact tcacgattaa tcgccatgtt
      121 gagtcctcac ttttcggcgc gtcacgcgct ggggatggcc ggcacgtcct gcccggtatt
      181 gcccgtgact tccggcacgg gggaagtcgg gagactgtat tgcggttgct ggagatggcc
      241 ggggttgacc cagccgcgcc gggacagccc cgtgtaggcc tcggcccgga tcacgcgttt
      301 gcagcgcgcg cagtagtctt gccagtggtg ctgctcttgc ggcagccaga aattatagcc
      361 gaccccgttg agggcctgct tcacgtcgtt gacctggatc tgcatgccga acggccggcc
      421 acagcgggcg cagttgcgca tgccctgctc ggtgcccacg gcgtaataga cctcgacacc
      481 caggctggcc gggcgctcga tgatgtggaa cagcttgccg aagggcaggt agaggatcat
      541 catgatcacc aggaactggt gggtcagcgt gatcggccag tgcatccagc ccgcgaagaa
      601 gaagtccacg atggtgagca tgatcccggt gatcgagatc gccatcagca gatgcagcgg
      661 cagcagatcg aacaacacgc gctgctgcgg cttcaccgcc cggtcctgcc agcggcgcca
      721 gtaggcgata gccacgccga tgatcaccag caccgcgaaa tagtcgagcg cgtggaaggt
      781 gttgaagccg accgggccga gcgagtccat ggtgatggtc gggaagccaa ggagatagac
      841 ccggtaggtg ctgccgccga cgacctcgaa atgcacccag ccgaaggtca gcgggaaggt
      901 cacggcgaag ctgaggatca cgccccagaa caggcactgg tgcatgatcc agcgcagggt
      961 gctgcgcttg ctgatgaagc gctggaacag caggttgctg atgaaggccc gcgggatgac
     1021 ccagaagtag cggcgggcat tttcgggctt caggaagaac tgccagctcc ggcgccacat
     1081 ggtgcgcgtg ctcggccgca tcagccaggt gccgtagtgg aaggccaccc cgaaggcggc
     1141 aaataggctg gcaatcgcgt acccggtcag cgccgggtcg aagaaggtca gcatccgcga
     1201 tccgaacagg atcgccagcg ccagcaggac catggcgccc aacccgatca gcgaggcgcg
     1261 gatcgtggta ttctggcgcg aatagagggc cgtgttcggc gcgatggggc gtgtcgtttc
     1321 ctgcatgggg ctcctttcat cgccaacaac cggacaaacc aacaacctga cctgggcagc
     1381 ggcataccgc gctctcgtgt tgcgcgttca ttataacaga gccggatgcg taatctcgta
     1441 gaaattaggt gaatatttcg tgaacgtttc cccatgcgct ccgggtcgtc acccggctta
     1501 ctctgtcgcg atctgtggcg aaacgttcac cttgtcttca ccgtctgaca ccgatctatt
     1561 tatcgccgga cgctagaatt accgcagcgt tttcgctgag gcgagcgcgg gagactgccc
     1621 cgcctgccgg cgcgcaagac cgtattggaa aggagtgttt cgtgcaacat ccgggccacg
     1681 agtgtggaaa atgcgcttcc cggcgcctgc gttttgccgg cacatccgcg tgcatggcgg
     1741 cccgctgccc ggttggtccg gctgcgcgct gtccggagta acgcggtatg gcgcaagctg
     1801 tcctggaatc accgtttgtc caattgctgc tgctcggggt ggcatccgcc gccgtgcggt
     1861 acttcttgtt gcgccggggc gagtcggcca cggcctccgg ggcgatcgcc atggcgatcg
     1921 gcatctttgt gctgggcgcg ttgcagcata tgcccttccc ggtgaccgat ctgacgcaga
     1981 tcctgacgct caccggcttg atcgtctggg cctatatcgc cgcctcgtat gtgcgctcgt
     2041 atttccgcgg cacctttcac aatcatacgg acgaccccgt cggatgcttt gcggtgggga
     2101 cgtgggtggc cggcaccgcc gtgctggccc gcctggtcgt catcgtgcta cccacgtggc
     2161 gcccgctggg gctggcgctc gccgtcctga ccctggtggt ctggctgtgg tatttgacgc
     2221 tgatcgttgg gcgctaccgg atcatcctgg ccagcccgac ccggctgcgc accaccgggc
     2281 gcatcctgct ggcaaccgtc agcacgcagt cgctcgtgtt gctgatcgag cagttgttcc
     2341 ccggccagat ccccggcggg ctggcgacgg gcctgatcct gttgggctac gggttctatg
     2401 gcctgggctt cgtgctcatc gtccagcgct atctgcgcca gcccggctgg cggctggccg
     2461 aggattggga caacacgaac tgtatcttgc acggggccat gtcgatcacg gtcctggccg
     2521 cgctggagag ccgggcggtg cccgacgcgc tcatcatcgc ttcgtggctc tgggcggcag
     2581 tgctgttcgt cattgtggag gccatcgaaa tcgcgcgcct ggtggcccgt gtgcgcgcct
     2641 atggctggcg gggcggcttc gccgtctatc aggtgtcgca atgggcgcgc aactttacct
     2701 ttggcatgtt ttacgccgcc acggcgcgct tacaggggct gggcgggacg gcggggctgg
     2761 gcctcgccgg tctctcgggt ctgcaagcgg cgatcgtggc ctggggccag tacatggtcc
     2821 tgatcttgtt agtaatcgag attagcttgt tcatgttgca caacgccggg ccgcgcctgc
     2881 aaaagctgtt cgcgcccgca ccgagtatga ccggggccgt ggaaacgcca caacattagc
     2941 aattgtttta gggggtactg ccatgcaaaa ctcacgccaa gcgtatgtaa acctcgggct
     3001 agcgaccctg gccttcgccg tcgctttcat cgcctggagt ctcatttcgc cgctcgccag
     3061 ccagatccag aaggattatg gcctcgataa taccgcgatc agcttcttga tcgccgtgcc
     3121 ggtgatcctc ggcagcctgc tgcgcattcc gatgggcatc ctgaccgacc gcttcggcgg
     3181 gcgcacggtg ttcaccgggc tgctgctctt cacgctgctg ccggtggctt tcctgggcat
     3241 cgccgggtcg ttctggacct acgtgctcgg cgccttcttc ctgggcacgg ccggggccag
     3301 cttcgctgtc ggcgtgccgt tcgtcaatcg ctggttcacg cccgataagc aagggctggc
     3361 cctgggcatc tacggcatgg gcaatatcgg caccgccgtg gccgccttct ccatgcccgg
     3421 cctggtcggc gcgttccagg gccgcgcagg cgccttctgg ttctacctgg tgcccgtgct
     3481 gctcatggcc gtgctcttct ggttgctggc cc
//
LOCUS       DASUYC010000362         4029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1422845, whole genome shotgun sequence.
ACCESSION   DASUYC010000362 DASUYC010000000
VERSION     DASUYC010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422845"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            290..2803
                     /locus_tag="VF276_05970"
     CDS             290..2803
                     /locus_tag="VF276_05970"
                     /inference="COORDINATES: protein motif:HMM:NF019291.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEX6606448.1"
                     /translation="MGGFAVSVRQWVARARTWGRARAQRSGAAEESQAAAGLALLRLL
                     GFELVGRAIRGGGESPLLPALWAAYQASGARDRFFAEWFPALQAACAGPTARPPANAA
                     VRALLPDPPDLRGTFLVSDPARPDPAALLTDTDAAAFFALLETCPPGVGGPALLAAVY
                     ESLAPTGAGVIYTARPEIDLMCRLAVVDSLTQHVGEAHRARLYAAVFAGDADAGAGLG
                     PAVWAWLDRVRVLDPACGAGAFLVGMLAVLAELASWAAPAPAMPRLSPADLALTALHG
                     VDIQPQAVQVAALCLWLQVAATAPGTALPAPVALHLRAADSLLDAPFPDQPAFDIVIG
                     NPPYVRQEAIGPRAYKSAVRRAVYHRFPAFFGYDAARDRAARPLDARGDLYLYFFFQG
                     VARLAPGGTLCFLTSNAWLDVRYGARLRAFLAETAHVRLVIDDQAQRSFAAEINTVIV
                     LLRGTPPAPAAWTRFVRGAVPLAALRAPATWAAIDAATTPRQTAEYAVVPVRQAQLAG
                     ALGPAPAGPEIGAGWGGAYLRMPDILRLVLDRAGDRLVPLAALAPVQPGLYTGINEFF
                     YLDAARIAAFGIESEFLVPVIRSPRAVPRLRIDADALASWLFVCREDKAALAARGRTG
                     ALTYIAWGETQVTRARQKVPAGVPWPAVPTVARRHPGWWALAPPPPAHLFLLYGIGAR
                     HGQPYSPVPLLSDRRFHMLAYAAPPADRETLAAVLNTILTVLCIELQGRTNLGQGVLD
                     FATMDARRLRVLDPARLAPTEQAALREGLHAVWAAPLPALPDPAYRATFAPLNGALAR
                     VLRLTPGEEDALFAAARELVAGRLRKAASIGRAGRRAAG"
     gene            complement(2816..>4029)
                     /locus_tag="VF276_05975"
     CDS             complement(2816..>4029)
                     /locus_tag="VF276_05975"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX6606449.1"
                     /translation="AQTLWPVDMQAEVHMEREIVIAGAGVAGLTAAINLKRAGRTVRV
                     LERSAASGAQRFPDWDAVENWTSLEDLPRFLERIGVEGTRFRHAGQTDFSVIDPYGRR
                     YDIQTPRPFFYLIKRGAVDGGLEHGLQAQAEAMGIPIEYGTPCPPGRADIWAGGTFGQ
                     GSLVVSAGFTFRTSHPDWTCGIVDARLAPRAYAYVVIVAGEGTLATLVTERSARREAN
                     ALLERAVTVFQQHAPALDIRDRHRAGGSGGDLAAFWQGRREFVIGEAGGYQDFLWGFG
                     IRHALTTGYLAAQAVITGQDWQQVADREIRPLVRASLVNRWLYDRMPPWGYARLMHHF
                     AGAPDLNMLISRWYHPRRIHRVLWPLVARTYQRETAALAGHERYTNRTRSAPALSAEA
                     DAPPAPSAVAD"
BASE COUNT          485 a         1527 c         1411 g          606 t
ORIGIN      
        1 acgccagcgc cagtaaggcg ccccgccgcg ggaccccatc ccgcggcgcc atgctacccg
       61 gccgggcacc ctcgcccggc ccgtgttatg ttcgcggcag cttccccgcc aaacttcggc
      121 taatcccacc tgccccgcgt atcccgtata atagccccat cagctacccg gcacgcagaa
      181 caaccactcg gcacgcctat gatcaccggt attcaaaatg ccacaggcga caacggacca
      241 gggaccgggt gcgggttgcg ctgcccctgc tcacaaggac gcgcctgaaa tgggcggttt
      301 cgcagtcagc gtccggcaat gggtggcgcg ggcccgcacc tggggccggg cccgcgccca
      361 gcgcagcggc gctgctgagg agagccaggc cgccgccggg ttggccttgc tgcgcttgct
      421 gggttttgaa ctagtgggcc gtgccatccg gggcggtgga gaatctcccc tcttgcccgc
      481 gctctgggcg gcctaccagg cgagcggcgc ccgtgaccgc ttcttcgccg aatggttccc
      541 cgcgttgcag gcggcatgcg ccggtcctac cgcccgccca cccgccaacg ccgccgtccg
      601 cgcgctgctg cccgacccgc ccgacctgcg cggcaccttc ctggtctccg acccggcccg
      661 gcccgatccg gcggcgctgc tcaccgatac ggacgcggcg gctttcttcg cgctcctgga
      721 aacctgtccg cctggggtgg gtgggccggc cctgctggcc gccgtgtacg aaagcctggc
      781 tcccaccggc gccggggtga tctatacggc gcgcccggaa atcgacctga tgtgccggct
      841 ggcggtggtc gatagcctga cgcagcacgt gggcgaggcc caccgcgccc ggctctacgc
      901 cgcggtgttt gccggggatg ccgatgccgg cgccgggttg gggccggcgg tgtgggcgtg
      961 gctggaccgg gtgcgcgtgc tcgatcccgc ttgcggggca ggcgcgtttc tggtggggat
     1021 gttggcggtg ctggccgaac tggcgtcgtg ggcggcgccg gcccccgcca tgcctcggct
     1081 ttcgcctgcc gatctcgccc tgacggcgct ccatggggtc gatattcagc cgcaggccgt
     1141 gcaggtcgcc gcgctatgcc tctggctcca ggtggccgcg acggcgcccg gtacggccct
     1201 cccggccccg gtggccttgc atctgcgcgc cgccgacagc ctgctggacg cgccgttccc
     1261 cgatcaaccg gcgttcgaca tcgtcatcgg caacccgccc tatgtgcgcc aggaggccat
     1321 cggcccgcgc gcttacaaaa gcgcggtgcg ccgcgccgtc taccaccgct tccccgcctt
     1381 cttcggctac gacgccgcgc gggatcgtgc cgcccgcccg ctcgacgcgc ggggcgacct
     1441 gtacctgtac ttctttttcc agggcgtggc ccggctggcg cccggcggca cgctctgctt
     1501 cctgacctcc aacgcctggc tcgacgtgcg ctacggcgcc cggttgcgcg ccttcctggc
     1561 cgagacggca cacgttcgcc tggtgatcga cgaccaggcg cagcgctcgt ttgccgccga
     1621 gatcaacacg gtgatcgtgc tgctgcgcgg cacgccgccc gcgcccgctg cctggacgcg
     1681 cttcgtgcgc ggcgccgtgc ccctcgccgc cctccgcgcc ccggcgacct gggccgctat
     1741 cgacgccgct accacgcccc ggcagacggc ggaatacgcg gtggtcccgg tgcggcaagc
     1801 acagttggcc ggcgcgctgg ggcctgcgcc cgccgggccg gagatcgggg ccgggtgggg
     1861 cggcgcctac ctgcgtatgc ccgatattct ccgtctggtg ctcgaccggg ccggcgaccg
     1921 gttggtcccg ctggccgcgc tggccccggt gcagccgggc ctttacaccg gcatcaacga
     1981 gtttttctat ctggatgcgg cgcgcatcgc tgccttcggc atcgagtccg agttcctggt
     2041 gccggtgatc cgttcgccgc gcgccgtgcc ccggctgcgc atcgatgccg atgccctcgc
     2101 tagctggctg tttgtctgcc gggaggacaa ggccgcgctg gcggcgcggg gccggaccgg
     2161 cgcgctcacc tacatcgcct ggggcgagac gcaggtcacg cgggcacggc agaaggtgcc
     2221 cgccggggtg ccctggcccg ctgtgcccac ggtggcgcga cgccatcccg gctggtgggc
     2281 gctggcgccc ccgcccccgg cccacctgtt cttgctctac ggcatcggcg cccgccacgg
     2341 ccagccctac agccccgtgc cgctgctgag cgaccggcgc ttccacatgc tggcctatgc
     2401 cgccccgccc gccgatcggg aaaccctggc cgccgtcctc aacacgatcc tgaccgtgct
     2461 ctgcatcgag ctccagggcc gcaccaacct gggccagggc gtgctcgact tcgccacgat
     2521 ggacgcccgg cgcctgcgcg tcctcgaccc cgcgcgcctg gcccccaccg agcaggccgc
     2581 gttgcgcgaa gggctccatg ccgtctgggc cgccccgctc ccggccctgc ccgaccccgc
     2641 ctaccgggcc acgttcgccc cgctcaacgg cgctctcgcg cgcgtcctgc gcctgacgcc
     2701 gggcgaagag gacgctttgt tcgcggcggc ccgcgagctg gtggccgggc ggctgcgcaa
     2761 ggcggcgagc atcggtcgcg ccggccgccg ggctgccggt tgagcgaccg cccgatcagt
     2821 cggcgacggc ggacggggcg ggcggcgcgt cggcttcggc ggacagggcg ggcgcggaac
     2881 gtgtccggtt ggtatagcgc tcgtgcccgg ccagggccgc cgtctcccgc tggtaggtgc
     2941 gggccaccag cggccagagc acgcggtgga ttcgccgcgg gtggtaccag cggctgatca
     3001 gcatgttcag gtcgggggcg ccggcgaagt ggtgcatcag ccgggcgtag ccccagggcg
     3061 gcatgcggtc gtagagccag cggttgacca gcgaggcgcg caccagcggg cggatttccc
     3121 ggtcggcgac ctgctgccaa tcctgcccgg tgatcaccgc ctgggcggcc aggtagcccg
     3181 tggtgagtgc gtggcggatg ccgaagcccc agaggaagtc ctggtagccg cccgcctcgc
     3241 cgatgacgaa ctcccgccgg ccctgccaga aggcggccag atcgccgccg ctgccgcccg
     3301 cccggtgccg gtcgcggatg tcgagggccg gggcgtgctg ctggaacacg gtcacggccc
     3361 gctccagcag cgcgttcgcc tcgcgccggg cgctgcgttc cgtcacgagc gtggctagcg
     3421 tgccctcgcc cgccacgatc accacgtagg cgtaggcgcg aggcgcgagg cgcgcgtcga
     3481 cgatgccgca ggtccagtcg gggtggctgg tgcggaaggt gaatcccgcc gacacgacga
     3541 gcgatccctg gccgaaggtg ccgccggccc agatgtcggc gcggcccggc gggcagggcg
     3601 tgccgtactc gatggggatg cccatggcct cggcctgcgc ctgtagccca tgttccaggc
     3661 cgccgtccac ggcgccacgc ttgatcaggt agaagaaggg gcgcggcgtc tggatatcgt
     3721 agcgccgccc atagggatcg atgactgaaa aatcggtctg gcccgcgtgg cggaagcgcg
     3781 taccttcaac gccgatacgt tccaggaacc gcggcaaatc ctccagcgag gtccagttct
     3841 cgacggcgtc ccagtcggga aagcgttgcg cccccgacgc cgcgctgcgc tccagcaccc
     3901 gcacggtgcg ccccgcgcgt ttgagattaa tcgcggcggt caaccccgcc accccggccc
     3961 cggcgatcac gatctcgcgc tccatatgca cctccgcttg catatccacg ggccacaacg
     4021 tctgcgccg
//
LOCUS       DASUYC010000363         2006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1978155, whole genome shotgun sequence.
ACCESSION   DASUYC010000363 DASUYC010000000
VERSION     DASUYC010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1978155"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..922
                     /locus_tag="VF276_05980"
     CDS             2..922
                     /locus_tag="VF276_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011142485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HEX6606450.1"
                     /translation="MDDAGRDGESSKEEIWPALDYEAWQDTYETLHMWTQIVGKIRLA
                     LSPMVNHWWQITLYVTPRGLTTSAIPYGPRTFEILFDFLNHNLGVYTSDGATRYMALY
                     PRSVADFYRELMAILSTLGINVQINPTPQEVPNPIACNIDTVHAAYDAEYANRFWRIL
                     VQTDSVFKEFRGRFLGKCSPVHFFWGSFDLAVTRFSGRRAPDRPGADHIQREAYSHEC
                     SSAGFWPGSGSMLAPAYYAYTVPAPDGLPQARIRPDSAYYDTALGEFLLRYDDVRRAA
                     DPRAMLLDFLQSTYEAGADLGRWDRAALER"
     gene            1037..>2006
                     /locus_tag="VF276_05985"
     CDS             1037..>2006
                     /locus_tag="VF276_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase"
                     /protein_id="HEX6606451.1"
                     /translation="MADPPFASFFLAGFECSTHRRLDGRRLDLIAATEHDRFADADYA
                     RCGAWGLHTVRDGIRWHLIERQPGRYDFSSVLPALRAAQAGGVEVIWDLFHYGWPDDL
                     DFFAPAFVTRFAALAGAFARLVVEETGRAPLVAPVNEISFVAWGAGTDGFMNPFALGR
                     GAEIKAQLVRAAIAAIEAVWAVAPAARILHPDPLIHVAPDPDKPEDHEAAGHAHRAQY
                     ESWDMLAGYRRPELGGHPRYLDIVGVNHYPWNQWIHNGPEIKAEHPLYRPLRDLLAEV
                     YARYRRPLLLSEVGTEGDGRAPWLRYVGREARAALRAGVPIAGVCLY"
BASE COUNT          335 a          674 c          652 g          345 t
ORIGIN      
        1 gatagatgat gcggggaggg acggtgaatc aagcaaggaa gagatctggc cggcccttga
       61 ttatgaagcc tggcaggaca cgtatgagac gctgcatatg tggacccaga tcgtaggcaa
      121 gatccggctg gcgcttagcc cgatggtcaa tcactggtgg cagatcacgc tctatgtcac
      181 gccgcgcggg ctgaccacct cggccatccc ctacgggccg cgcaccttcg agatcctgtt
      241 cgacttcctc aaccataacc tcggcgtcta caccagcgac ggcgcgaccc ggtatatggc
      301 cctctatccg cgctcggtgg ccgactttta ccgggaactg atggcgatat tgagcacgct
      361 gggcatcaat gtgcagatca accccacgcc gcaagaggtg ccgaatccca ttgcgtgcaa
      421 tatcgataca gtccatgccg cctatgacgc agaatacgcc aaccgcttct ggcgtattct
      481 cgtgcagacc gacagcgtct tcaaggagtt tcgcggacgg tttctcggca agtgcagccc
      541 ggtgcacttt ttctggggta gcttcgatct ggccgtgacg cgcttctccg ggcgccgcgc
      601 ccccgaccgg ccaggggccg accatatcca gcgcgaagcg tactcgcacg agtgcagcag
      661 cgccggattc tggccgggca gcggcagcat gctcgcgccg gcatactatg cgtatacggt
      721 gccggctccg gatggcctgc cccaggcgcg catccgcccg gatagcgcat actatgatac
      781 ggccttgggc gagttcctct tgcggtacga cgatgtccgc cgggcagcag atcccagagc
      841 catgttgctc gattttctgc aaagcaccta cgaggccggt gccgatctgg ggcggtggga
      901 ccgcgcagcc ctggagcgat aaagccggct taagacgaga gaatcgcatt tgcagagcgt
      961 ggagcatgga aagggcagtg aaacgccagg gggtgcgccg cagcctcgta gccacaagaa
     1021 taggatcgcc tcgactatgg ccgacccacc cttcgcgagt ttctttctgg ccggcttcga
     1081 atgttcgacc caccggcgcc tggatggccg ccggctcgac ctcatcgccg ccaccgaaca
     1141 cgaccggttc gcggatgccg actatgcgcg ctgcggcgcc tgggggttgc atacggtacg
     1201 cgacggcatc cgctggcatc tgatcgagcg gcagcccggt cgctacgact tctccagcgt
     1261 cttaccggct ctgcgcgcgg cccaggccgg cggcgtggaa gtgatctggg acctgttcca
     1321 ctacggctgg cccgacgatc tcgacttctt cgcgcccgcc ttcgtcacgc gctttgcggc
     1381 gctggccggc gctttcgccc ggctggtggt cgaggaaacc gggcgcgcgc cgctggtggc
     1441 gccggtgaac gaaatctcgt ttgtcgcctg gggcgcgggc accgacggct tcatgaaccc
     1501 cttcgcgctg gggcgcggcg ccgagatcaa ggcgcaattg gtccgcgccg ccatcgccgc
     1561 catcgaggcc gtctgggcgg tcgcgcccgc cgctcggatc ctgcatcccg acccgctgat
     1621 ccacgtcgcg cccgatccgg acaagcccga agaccatgaa gccgccgggc acgcccatcg
     1681 cgcgcagtac gaaagctggg atatgctggc cgggtaccgg cggcccgaac tgggcgggca
     1741 cccgcgctat ctcgacatcg tgggcgtcaa tcactatccc tggaaccagt ggatccacaa
     1801 cggcccggag atcaaggcgg agcacccgct ctaccggccg ttgcgcgacc tgctggccga
     1861 ggtctacgcc cgctaccggc gcccgctgct gctgagcgag gtcggcaccg agggcgacgg
     1921 gcgggcgccc tggctgcgct atgtcgggcg cgaggcgcgc gccgcgctgc gggccggggt
     1981 tcccatcgcc ggggtctgcc tctacc
//
LOCUS       DASUYC010000364         3259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_998917,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000364 DASUYC010000000
VERSION     DASUYC010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998917"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="VF276_05990"
     CDS             <1..892
                     /locus_tag="VF276_05990"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX6606452.1"
                     /translation="VALEMGSFGRVAWQHGDYATARRQLQEALAVQRAVGDKWNVAWL
                     LRNLGDVARYEGDYAQARILLEESLALFRDLADQWGINNTLYLLGLVAQQRGDLGQAT
                     VLLRESLSLARETGDQWVSVQCLLALAAIAVAAGQVERGGVLGGAAEALFAVAGTYLE
                     PAERAEAARNLATLRRPAGSSFTAGWAQGRALPLTQAIALALQDLSIPGDRPQAAGPI
                     GKPAPAPANTLRTDPDALTPREIEVLRLVAAGMTDAAIARQLVLSPRTVQVHLRSIYS
                     KLNITTRSAATRYALEQHLA"
     gene            1236..2375
                     /locus_tag="VF276_05995"
     CDS             1236..2375
                     /locus_tag="VF276_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606453.1"
                     /translation="MNSEHTGTRFTWTDVNQWVETGLIRPGQALAIRRHVAALGAVAQ
                     QAQTGIEQRPGLNIITVAYYFGGFMILLAYTFFMGFQWETISRLGQCVVVGCTIGLLW
                     ALGYLLRRVGFRLAGGLLTFAGTGIVPLLVYTILHLTGIWPDQPSYDSPAYEDFYLQV
                     KPAWVTMEAVSMVVAAVVTLRLRFPLMTLLVGFWGWYLSMDLTRWIFQTPDWSYDEHE
                     QIAGIIMGLIMLAIGLGLQFRTHQDYSRWLYIFGHVVVLGNFSALAHDQEGLAALLYV
                     GLYLTFVAASVWLQRPVFVVFGALGCYGYASYLAYDKFHGSLPFVFTLALIGLTIVLA
                     GVGYQKYGRPWRLRRSAAKQSIAARESGDVPVAIGTATTTAPAEE"
     gene            2526..>3259
                     /locus_tag="VF276_06000"
     CDS             2526..>3259
                     /locus_tag="VF276_06000"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6606454.1"
                     /translation="MQTPVEPAVYGHPERSELIGTTVDAPSTPLAAESAPAVPGAQPF
                     NTGQAIRYSVANFGSSNFYMLFNTAMPLYLDTYHLDPALIGLLANERSLVGALIQPIV
                     GRISDRTRTPLGRRRPFFLIGVPLVSLALLLLALHPPFWLMLGVMTIAAFFLAVAVDP
                     YLALLADLCPPAQRGRVAGFTGLANALGAITFSLLAVLLWAHYEFLVFALVIILLLGA
                     WGFTFFTVKEPPPPPAPRVEPAARRSA"
BASE COUNT          555 a         1038 c         1051 g          615 t
ORIGIN      
        1 ggtggcgctc gaaatgggta gcttcgggcg ggtggcctgg cagcatggcg attatgccac
       61 ggcgcgccgg caactccagg aggcgctggc cgtgcagcgg gccgtggggg acaaatggaa
      121 tgtagcctgg ctgctgcgga atctggggga tgtggcccgc tatgaaggcg actatgccca
      181 ggcgcggatc ctcctggagg agagcctggc gctgtttcgt gacctggcag atcagtgggg
      241 gatcaacaac accctgtatt tgctcgggct cgtcgcccag caacgagggg atctcggcca
      301 agccacggtc ctgttgcgcg agagcctgtc gctggcgcgg gagaccggcg accagtgggt
      361 gagcgtccag tgcttactcg cgctggccgc catcgcagtc gccgccgggc aagttgagcg
      421 cggcggcgtg ctggggggcg ccgcggaggc cctgtttgca gtcgccggga cgtatttgga
      481 gccggcagaa cgggccgaag ccgcgcgcaa tctggcgacc ttacgccgcc cggcgggatc
      541 gagtttcact gcgggctggg cgcagggccg agccttaccg ctaacccagg ccatcgcgct
      601 ggcgctccag gacctgtcga tcccggggga tcggccgcag gcagccggtc cgatcggcaa
      661 gcccgccccg gccccggcta ataccctgcg gaccgatcct gatgcgctca ccccccgcga
      721 gatcgaggtg ctgcgcctgg tcgcagcggg gatgaccgat gccgcgatcg cccggcaact
      781 cgtgctcagt ccgcgcaccg tgcaggtgca tctgcgttcc atctacagca agctgaatat
      841 caccacgcgc agcgccgcca cccgctacgc cctggagcag catctcgcct gaccggcctt
      901 ccccagcccc atggtctgca ccagccccac agacggaatc gagaggacgt gggggagcag
      961 tggcgctgca gccatgatct gcgaccgtct atcggactaa cgctatagac aaaccaaccg
     1021 cggacctggc gacggtgcga cagcaagggt agaccagata agcacaacta cctacattga
     1081 cctcccacta ctgctcccag cacgcgcgaa aaattacgca acactgcaga tggccgcggc
     1141 gccccccggt gctatagtct tctcaagaac ctgacttggc tcgctggcag ccacagaatc
     1201 aagcgggtgg gcatatatcg gaaaaggagc ctctgatgaa cagcgagcat acgggaacac
     1261 ggtttacatg gaccgatgtt aaccagtggg tggagaccgg gttgatccgg ccaggccagg
     1321 cgctagcgat tcggcggcac gtggctgcgc tgggggccgt cgcccagcag gcgcaaaccg
     1381 gcatcgaaca gcggcccggg ctgaatatca tcaccgtcgc gtactacttc ggcgggttca
     1441 tgatcctgct ggcgtacacc ttctttatgg gcttccagtg ggaaaccatt agcaggctgg
     1501 gccagtgtgt cgtggtgggc tgcaccatcg ggctgttgtg ggcgctcggt tatctgttgc
     1561 gccgggtcgg ctttcgacta gcgggcgggt tattgacctt cgccggcacg ggcatcgtac
     1621 ccctgctggt ctataccatc ctccacctga ccggaatctg gccggatcag cccagctatg
     1681 acagccccgc gtatgaggat ttctatctac aagtcaaacc cgcctgggta accatggaag
     1741 cagtcagcat ggtagtggcc gctgtggtca cgctacggct acgcttcccc ctgatgaccc
     1801 tcctggtcgg cttctggggt tggtatcttt cgatggactt gacccgctgg atcttccaga
     1861 cgccggactg gtcgtatgac gagcatgaac aaatcgccgg catcatcatg ggcctaataa
     1921 tgctggcgat cggcctgggg ctgcagttcc gcacccatca ggactatagc cgctggctgt
     1981 acattttcgg gcatgtggtg gtgctgggga atttctccgc gctggcccat gaccaagaag
     2041 gactcgccgc gctcctatac gttgggctct acctgacgtt tgtggccgca agcgtctggc
     2101 ttcaacggcc ggtcttcgtc gtgttcggcg cgctcggctg ctatggctat gccagttacc
     2161 tggcctatga caagttccac ggttcgctcc cgttcgtctt cacgctggcc ctgatcgggc
     2221 tgacgattgt cctggccggg gtgggctatc agaagtatgg gcggccctgg cgcctgcgac
     2281 ggagcgccgc gaagcagagc atagcggcgc gggaaagcgg ggatgtgccg gtagctattg
     2341 ggacggcgac gaccaccgcg ccggccgaag agtaaaacta gcctacataa aggagtcaat
     2401 acctggcaac cggcggataa agggcagcgg cgctgtggaa cctgtgcagc gccctgctgc
     2461 cggggcagca cctgatatgc aatgagcagt gtagaaacga acgttcagaa agtgagcgca
     2521 ttgccatgca aactccagtg gaacccgcgg tctatggcca tcctgagcgt agcgagttga
     2581 taggcacgac cgtcgatgcg cccagcacgc cgctagcggc ggaatccgcg ccagccgtgc
     2641 ccggcgccca accgttcaat acgggccagg ccatccggta cagcgtggcc aatttcggct
     2701 cctcgaactt ctatatgctg ttcaacacgg ccatgcccct gtatctcgac acgtaccatc
     2761 tggacccagc gctgatcggg ttgctcgcca acgagcgctc gctggtgggg gccctcatcc
     2821 aacccatcgt cgggcgcatc agcgaccgga cgcggacccc gctgggtcgg cgccgcccgt
     2881 tcttcctgat cggcgtgccc ctggtgtcgc tggcgctgct gttgctggcc ctgcacccgc
     2941 ccttctggct gatgctgggt gtgatgacca tcgcggcctt ctttctcgcg gtggccgtcg
     3001 acccctatct ggcgctgctg gccgacctgt gtccgcccgc gcagcgcggc cgcgttgccg
     3061 ggttcaccgg gctggcgaac gcgttgggag cgattacctt ctcattgctc gccgtcctct
     3121 tgtgggcgca ctatgagttc ctggtgtttg ccctggtcat catcctgctg cttggagcgt
     3181 ggggctttac gttcttcact gtcaaagaac cgccgcctcc gccggcgccc cgcgtggaac
     3241 ccgcggcccg ccgcagtgc
//
LOCUS       DASUYC010000365         2586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_358529,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000365 DASUYC010000000
VERSION     DASUYC010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358529"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VF276_06005"
     CDS             <1..354
                     /locus_tag="VF276_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009361851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX6606455.1"
                     /translation="RYPDFAQGGEIVRHHHERWDGTGYPHRLKGADIPFGARVVAVAD
                     SFDAMTSDRPYRRALPVRKAAEILRAGRGTQWDPVAVDAFLRSIGDRLERPAPLLLKV
                     GAETPEPEPVIAARA"
     gene            complement(446..1240)
                     /locus_tag="VF276_06010"
     CDS             complement(446..1240)
                     /locus_tag="VF276_06010"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEX6606456.1"
                     /translation="MSAAEHQPCATCGACCRSYIVPVCGYDMWLINRRQHLSPEQYVV
                     ACPQPEQGFDGVLLESGGQPYGLALDKQGRFGIKQPCVFLLRLADGNVRCGIYADRPL
                     VCQAYPMSMWSGVVFQGKRTLCPPDSWPAHEPRRPRWRATLQRVHMQFDLYQAVVTRW
                     NARVTARPGVRFTLREYFSYVLNVYDRLDGLATATGAETLAGVLAGWPLPPHQPFDLA
                     TLRAAGADLLWVDYLRQAAAIIDSFYPDVPPQPLHLLEPPPVPAAG"
     gene            complement(1237..1860)
                     /locus_tag="VF276_06015"
     CDS             complement(1237..1860)
                     /locus_tag="VF276_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606457.1"
                     /translation="MTELGLTRKEEIWLACKQKSCCYAAFVVPSGRDIWRIARTLDSP
                     PWAFLVYFPAPAPRRDAFHLDQSGVAYRVALAKGATRRKKAPPPCIFLLRTRNGAHRC
                     GLGALRPGVCKSFPSDLLGGVLYMQDGTGCACRTWSLADVDIAEETAAVTARRQDTAE
                     YCEVVARWNARVAAAPPGTQFSFFDYCDFLLEAYDALDAAPAGENGA"
     gene            complement(1879..2412)
                     /locus_tag="VF276_06020"
     CDS             complement(1879..2412)
                     /locus_tag="VF276_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606458.1"
                     /translation="MAESGILVQYVVRGPAAWGLRVLADGGVAELSNEPPGGAEGGDS
                     APLAWRPLIRLAPAGLDRLRAVLREADFAALPHHAPPPEAGVDTVITTWDIDLDGAHH
                     TITTVGREEGQHPTLESWRATLDHLLRAAFGAPTWPAADDATARRDWLHHPPWRVGAR
                     GGGPHNGHTPSSENGSA"
BASE COUNT          397 a          897 c          930 g          362 t
ORIGIN      
        1 cgctatccgg acttcgcgca agggggcgag atcgtacgcc atcaccatga gcgctgggac
       61 ggcaccggct acccccaccg gctcaagggc gccgacatcc cgttcggggc ccgcgtggtc
      121 gccgtggccg acagcttcga cgccatgacc agcgaccggc cttaccgccg ggccctgccg
      181 gtgcgcaaag ccgccgagat cctgcgcgcg ggccgcggca cccagtggga cccggtcgcc
      241 gtggacgcct tcctgcgcag catcggcgac cggttagagc ggcccgcgcc gctcttgctg
      301 aaggtgggtg ccgagacccc ggagccggaa cccgtcatcg ccgcccgcgc ctgaccgctt
      361 ctccggcaaa tacgcggcga tcacaatgcc ccggcgacct gtacggtcgc tggggcgtgc
      421 tttttaaccc gtggattccg ggtgcttatc cagcggcggg caccggcggt ggttcgagca
      481 ggtgcagcgg ttgcggcggc acgtcgggat agaagctgtc gatgatcgcc gccgcctggc
      541 ggaggtaatc gacccagagg agatcggcgc ccgcagcgcg cagggtggcg aggtcaaagg
      601 gctggtgcgg cggtagcggc cagccggcca gcaccccggc cagcgtctcg gcgccggtcg
      661 ccgtggcgag gccatcgagc cggtcgtaga cgttgagcac atagctgaaa tattcgcgca
      721 gcgtaaagcg gacgccgggc cgggcggtca cgcgggcatt ccagcgggtg acgacggcct
      781 gatagagatc gaactgcatg tgcacgcgct gcaacgtggc gcgccagcgc gggcgccgcg
      841 gttcgtgggc cggccaggag tcgggcgggc agagggtccg cttgccctgg aagaccacgc
      901 cactccacat cgacatggga taagcctgac agaccagcgg gcgatcggcg tagatgccgc
      961 agcggacatt gccgtcggcc aggcgcagca agaacacgca cggctgcttg atgccgaagc
     1021 ggccctgttt atccagcgcc agcccgtacg gctgcccgcc actctccagc agcacgccgt
     1081 cgaagccctg ctcgggttgc gggcaggcaa ccacgtattg ttcggggctg aggtgctgcc
     1141 gccggttgat cagccacatg tcgtagccgc agacgggcac aatataggat cggcagcagg
     1201 cgccgcaggt ggcgcacggc tggtgttccg cggcgctcat gccccgttct ccccggcagg
     1261 cgccgcatcc agcgcatcgt aggcttccag caagaaatcg caataatcga agaagctgaa
     1321 ctgcgtgccg ggcggcgcgg cggcaacgcg ggcgttccag cgggcgacca cctcgcagta
     1381 ttcggcggtg tcctggcggc gggcggtgac ggcggcggtt tcctcggcaa tatcgacatc
     1441 ggccagcgac caggtgcggc aggcgcagcc ggtgccgtcc tgcatgtaga gcaccccgcc
     1501 cagcaggtcg gacggaaagc tcttacacac ccccggccgc agggcgccca ggccgcagcg
     1561 gtgcgcgccg ttgcgggtgc gcagcaggaa aatacagggc ggcggcgctt tcttgcgccg
     1621 cgtcgcgccc ttcgccagcg ccacccggta ggccacaccc gattggtcga ggtgaaaggc
     1681 atcgcgccgg ggcgccggcg ccgggaagta gaccaggaag gcccacggcg ggctatcgag
     1741 cgtgcgggcg atgcgccaga tgtcacgccc gctcggcacc acgaacgcgg cgtagcaaca
     1801 cgatttctgc ttgcaggcca gccagatttc ttctttgcgc gtcagtccca gttccgtcat
     1861 ccgttactcg tttctagctc aggcggatcc gttttcgcta gacggcgtat ggccgttgtg
     1921 cggcccgccc ccgcgagcgc cgacccgcca gggcgggtgg tgcagccagt cgcggcgggc
     1981 ggtagcatca tcagcagccg gccaggtcgg ggcgccgaac gccgcgcgca gcaggtggtc
     2041 gagggtcgcg cgccacgatt cgagcgtggg gtgctgcccc tcctcccggc ccactgtggt
     2101 gatggtgtga tgcgcgccgt cgaggtcgat gtcccaagtg gtgatcaccg tgtccacgcc
     2161 ggcctcgggc ggcggcgcgt ggtgcggcaa ggccgcgaaa tcggcctccc ggagcacggc
     2221 acgcaaccga tccagacccg caggcgccag ccggataagc gggcgccagg ccaggggcgc
     2281 tgaatcgccg ccttcggccc caccgggcgg ctcgttgctc agttccgcca cacccccgtc
     2341 ggccaggacg cgcaggcccc aggcggcggg cccgcgcacg acgtattgca ccagtatacc
     2401 agattcggcc atctgcctcc ccctggcggg ctaatcggac ccgccggagc gcgtgcggtc
     2461 gggcaccccg gcatccgcgg ggtcgccacc ggtgccgccg tcggtggccg gattgggcac
     2521 gccgcccggc aggctgccgc cgtcgagcac gctatcgtcg acgggcggcg gcgaggccgg
     2581 ggcaag
//
LOCUS       DASUYC010000366         2181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_76926,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000366 DASUYC010000000
VERSION     DASUYC010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76926"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VF276_06025"
     CDS             complement(<1..511)
                     /locus_tag="VF276_06025"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX6606459.1"
                     /translation="MTEQMGIFDVIYNTRAMRRIKPDPVPEELLLKLIDAANQAASGS
                     NAQNARYIVVRDRAQKERLGELNRRVFEAYTAPGGARAATMPHQTEVKRARMLEAVRW
                     QADHFAEIPAHIIACLQVALPLRDSFAAGAGAGGSIWPGVQNLLLAARALGLAATPTT
                     FAIADRNAAR"
     gene            620..1603
                     /locus_tag="VF276_06030"
     CDS             620..1603
                     /locus_tag="VF276_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606460.1"
                     /translation="MHQLPGLRLAPNWTSAAAALEGILEYYGQALPRHAVMGLTGHAW
                     HTCLGTKGEVTALPSGPADLDWEVMAARYARTGLRWERFGGELPGEGPWDEMRTAAIE
                     WGIAHLDAGRPLIGWDFHLHEHGIVYGYDRDRAGWLVHDMLTEELGPFVLWDVWPTAA
                     IGKLELLAPVETLPEQDPFETVGGALQTALDCFDGRDGPEDGQPRGTAAIDAWADAFE
                     GETEVDRAGNAYTLVVLQAARMDGAEFLANIAGSIPELAEPLLAAERSIREQVKALAP
                     LITLFPFPTGGHGNVNNPGLRRAAAMALRRAAQHERAAAEGIRAALDVLEG"
     gene            1670..1999
                     /locus_tag="VF276_06035"
                     /pseudo
     CDS             1670..1999
                     /locus_tag="VF276_06035"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
     gene            complement(2012..2176)
                     /locus_tag="VF276_06040"
     CDS             complement(2012..2176)
                     /locus_tag="VF276_06040"
                     /inference="COORDINATES: protein motif:HMM:NF018597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HEX6606461.1"
                     /translation="MTVLAGWWGADERERAFLRAVHQGGCETFSTVLGPQYDANHRDH
                     FHLDQEVRPR"
BASE COUNT          343 a          678 c          825 g          335 t
ORIGIN      
        1 cgcgggcggc gttgcggtcg gcgatggcga aggtggtggg cgtggcggca aggccgaggg
       61 cgcgggctgc aagcagcagg ttctggacgc caggccagat ggagccaccg gcgcccgcgc
      121 ccgccgcgaa cgaatcgcgg agtgggagcg ccacctggag gcaggcgatg atgtgcgcgg
      181 ggatctcggc gaaatggtcg gcctgccagc gcactgcttc cagcatgcgg gcgcgtttta
      241 cttccgtttg atggggcatg gtggcggcac gcgcgccgcc gggcgccgtg taggcctcga
      301 agacgcggcg gttgagttcg ccaaggcgct ctttctgggc acggtcgcgg acgacgatgt
      361 agcgcgcgtt ctgggcgttg gacccggagg ctgcctggtt ggcggcgtcg atcagtttga
      421 gcagcagttc ttcggggacg gggtccggct tgatgcggcg catggcgcgc gtgttgtaga
      481 tgacgtcgaa gatgcccatt tgttcggtca tgagggtgcg gctccccggt ggtgtagtga
      541 ttcccgacta taccggcacg gggcgcacgg cacggtcccg gagtgcgatt catagttccc
      601 aattgcctac actcagggtg tgcaccagct acccggcctt cgtcttgcgc cgaactggac
      661 cagcgccgcg gcagcgctcg agggcatcct cgagtactac gggcaggcac tgccgcgaca
      721 tgcggtgatg gggctcacgg ggcatgcctg gcatacctgc cttggcacga aaggcgaggt
      781 gaccgcgcta ccctccgggc cggccgacct cgactgggag gtcatggccg cgcgctacgc
      841 gcgaacaggg ctacggtggg agcgcttcgg cggcgaattg ccgggcgaag ggccgtggga
      901 cgagatgcgg accgccgcga tcgaatgggg aatcgcgcac ctggatgccg ggcggccgct
      961 catcgggtgg gactttcacc tgcacgagca cggcatcgtc tatggctatg accgcgaccg
     1021 ggcgggctgg ctggtacacg acatgctgac ggaggaactg ggcccgttcg ttctctggga
     1081 tgtgtggcca actgcggcca tcggcaagct cgaactgctc gcgccggtcg aaacgctgcc
     1141 ggaacaggac ccgttcgaga cggtgggtgg cgcgctccag acggcgctcg actgcttcga
     1201 cggcagggac ggcccggaag acgggcagcc gcgtggcaca gcggccatcg acgcctgggc
     1261 ggacgcgttc gaaggggaga ccgaggtcga ccgcgcggga aacgcctata cgctggtggt
     1321 gctgcaggcc gcgcgcatgg acggcgcgga gttccttgcg aacatcgccg ggtcgatccc
     1381 ggagttggcg gagccgctgc tcgctgccga gcggtccatc cgcgagcagg tgaaggcgct
     1441 ggcgccgcta atcacgctct tcccgttccc gaccggtggg cacggcaacg tgaacaatcc
     1501 gggcctgcgc cgggcggctg cgatggcgct gcgccgcgcc gcccagcatg agcgcgccgc
     1561 ggcggagggt atccgcgccg cactggatgt gctggagggc tgagatggcg gcgggccaag
     1621 gccgggccgc aagcggtttt cgccgtgctg gacgcgtaga atccgggcga tgaagacgta
     1681 tccaacctcc tccgaacttt caacgccgcg cggttacagc cacgtggttt ccgccacggc
     1741 cggccgcacc gtgtacgtcg ccggtcagat cgccgtcgac aaggacggca acgtggtcgg
     1801 gaagggcgac ttgcgcgcgc agacgacgca ggtactcgag aacgtgaagc acgcgctcgc
     1861 ggcgggtggg gccacgtttg cagacatcgt gaagcagaac acgtacatcg tgaacctgaa
     1921 gccggaagac ctgccgatca tccgcgaggt gcgcgcacag tacttcccgg cagagaaccc
     1981 gccggcgagc acgctggtgg gggtgacggc gctagcgggg ccggacttcc tgatcgaggt
     2041 ggaagtgatc gcggtggttg gcgtcgtact gcggcccgag cacggtcgag aacgtctcgc
     2101 agccgccctg gtgcacggcc cgcaggaacg cgcgctcgcg ctcgtcggcc ccccaccagc
     2161 cggcgagcac ggtcaccttg c
//
LOCUS       DASUYC010000367         5007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_943831,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000367 DASUYC010000000
VERSION     DASUYC010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5007
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943831"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="VF276_06045"
     CDS             <1..956
                     /locus_tag="VF276_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606462.1"
                     /translation="SAAAPSATTSADPAAAAKLSQAVEQLDQTSFTLTASSGPGFRMT
                     GSIDAPNGKAAAQLTASGPNAEINVKTILIGQDLYAQVPGITKAGTWTHVDGSRLPAN
                     ANIGLRPGQIDPVNTANLLKATTDVKATSDTSYTGSVDLSKAAGVAGLGQVTIAASDA
                     PVQNVPFTATLDAQGRLSTLTVDLPQAEPLQVKYENYGRPVNVAAPAAIARKKSCQRV
                     SSASNMGSIGSPPASWCLRSATGLSFAIRVGPGVATPCGLAAISNRTLTYLYRASSVP
                     PSILYVTHGRQGGQCELRALRSRLESLSFERPSLPRRGRLL"
     gene            970..2886
                     /gene="dnaK"
                     /locus_tag="VF276_06050"
     CDS             970..2886
                     /gene="dnaK"
                     /locus_tag="VF276_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX6606463.1"
                     /translation="MARVIGIDLGTTNSVVSVLEGGEPTVIPNAEGSRLTPSVVAMSK
                     SGERLVGQVAKRQAITNPENTIFSIKRFMGRKFNEPEVQRDMKLVPYKVSAAPNGDVQ
                     VWMGGRAYSPPEISAMILQKLKQDAETYLGETVTQAVITVPAYFNDSQRNATKDAGRI
                     AGLEVLRIINEPTAASLAYGLDKNDKEEELAVYDLGGGTYDISILRMAEGVFEVASTN
                     GDTHLGGDDFDQRIIDWLADEFKKEQGIDLRQDRMALQRLKEAAERAKIELSTTQSTE
                     VNLPFITADASGPKHMNITLTRAKLEQLVGDLIQKTIPPMKRALEDAKLKANQIDEVL
                     LVGGQTRMPKVQDEVRTFFGKDPHRGINPDEVVAVGAAIQAGVLQGEVKGILLVDVTP
                     LTLSVETLGGVSTPMITRNTAIPTKKSETYSTASDNQPSVEIHVLQGERPMASENKSL
                     GRFILDGIPPAPRGVPQIEVTFDIDANGTLNVTARDKASGKEQKITITASSGLSKDEV
                     DRMVKEAEAHAEEDKRRRELIEARNQADSLIYSTEKTLQEYGDKISADVKSDVEQKLA
                     ALREAREADDPARITSAVQDLAHAAQKIGEAMYGQPGGDGTGAEAGAGAGAGAGGYNP
                     PSDEGTVEGEFREV"
     gene            3080..4264
                     /locus_tag="VF276_06055"
     CDS             3080..4264
                     /locus_tag="VF276_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015407523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEX6606464.1"
                     /translation="MNLERFQQSPAGHIVQAVHRGTPYVAFVPNPLPPRLELDPALWR
                     SLSDADRALGELAGLGRTLPNPQLLINPFMRREAVLSSRIEGTQAGIADLYAYEASQL
                     ALPGFDESTPASDVQEVLNYVHALEYALEEVSRLPVSLRLLRNMHARLMEGVRGAHAT
                     AGAFRRIPNLIGHPGATLQNARYVPPPVPQMLDALDALEKYLHAQSEDDYPPLVRLAL
                     IHYQFEAIHPFEDGNGRSGRLLISLLLVNWGLLPAPLLYLSAFFERYRATYYDLLFAV
                     SERGTWREWVLFFLQGVSDQAADAIARAKRLQDLREGWRAQLLQGRTGATALHLLDTL
                     FVAPVITTRGAQDLLGVTYPAAKANIQKLVQAGILVPGPRASNGQTYVAEAILQILRD
                     EP"
     gene            complement(4375..4836)
                     /locus_tag="VF276_06060"
     CDS             complement(4375..4836)
                     /locus_tag="VF276_06060"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6606465.1"
                     /translation="MLTLRPMTEEEFEQFKGPNMEDYAQERARNFGTPIEQERAIAAD
                     QYARFLKDGVHTAGQRLWVVTGETGAVVGHLWVGIEDAEHMAFIYFIDINADQRGKGY
                     GAQTLDLLEAEMRPLGIRHIGLNVFSDNAVAQRLYARQGYRVTNYSMLKPI"
BASE COUNT          939 a         1627 c         1605 g          836 t
ORIGIN      
        1 cctcggccgc ggcgccgagc gcgacgacct ccgccgaccc ggcggcggcc gcgaagctgt
       61 cgcaggcggt cgagcagctc gaccagacca gtttcaccct cacggcgtcg tccggccccg
      121 gtttccggat gaccggttcg atcgacgcac ccaacggcaa ggccgccgcc cagctgaccg
      181 cgagcggccc gaacgccgag atcaacgtca agaccatcct gatcggccag gacctgtacg
      241 cgcaggtgcc cgggatcacc aaggccggca cctggacgca cgtcgacggc tcgcggctgc
      301 cggccaacgc caacatcggc ctgcgtcccg gccagatcga cccggtgaac acggcgaacc
      361 tgctcaaggc caccaccgac gtgaaggcga ccagcgacac cagctacacc ggctcggtgg
      421 acctgtcgaa ggcggccggg gtcgccgggc tgggccaggt caccatcgcc gcgtcggacg
      481 caccggtgca gaacgtgccg ttcacggcca cgctcgacgc gcaggggcgg ctctccaccc
      541 tgaccgtcga cctgccccag gccgagccgc tgcaggtgaa atacgagaac tacggccggc
      601 cggtgaacgt cgccgccccg gccgcgatcg ccaggaagaa gtcgtgccag cgcgtcagca
      661 gcgcgtcgaa tatggggtcc atcggcagtc ctccggcctc ctggtgcttg cgttccgcaa
      721 ccggcctcag cttcgccatc cgtgtggggc caggtgtcgc cacgccgtgc ggattggccg
      781 cgatctctaa cagaacgcta acatatctct atcgggcttc tagtgttcca ccaagtatac
      841 tgtatgtaac gcatgggcga cagggcgggc agtgcgagct acgtgctctg cgctcacgct
      901 tggaatcgct atcatttgag cgcccctccc tcccgcggcg ggggcgactt ctttaggagg
      961 attagtttta tggctagagt aattgggatc gacctgggca cgacgaactc ggtcgtatcc
     1021 gtgctcgaag gtggggagcc gacggtgatc cccaacgccg aaggcagccg gctgacgccg
     1081 tccgtcgtcg cgatgtcgaa gtcgggcgag cggctggtgg gccaggtggc gaagcgccag
     1141 gcgatcacca acccggagaa caccattttc tcgatcaagc gcttcatggg ccgcaagttc
     1201 aacgagcccg aagtgcagcg cgatatgaag ctggtgccct acaaggtcag cgcggcgccc
     1261 aacggcgacg tgcaggtctg gatggggggt cgcgcctata gtccgcccga gatttcggcc
     1321 atgatcctgc agaaactgaa gcaggatgcc gagacctacc tgggcgagac cgtgacccag
     1381 gccgtcatta ctgtgccggc ctacttcaac gacagccagc gcaacgcgac caaggacgcg
     1441 gggcgcatcg ccgggctcga agtgctgcgc attatcaacg agccgacggc ggcgtcgctg
     1501 gcctatggcc tggacaagaa cgacaaggaa gaggagctgg ccgtctatga cctgggcggc
     1561 ggcacctacg acatcagcat cctgcgcatg gctgagggtg tgttcgaggt cgcttcgacc
     1621 aacggcgata cgcacctggg cggcgacgac ttcgaccagc gcatcatcga ctggctggcc
     1681 gacgagttca agaaggagca gggcatcgac ctgcggcagg accgcatggc cttgcagcgc
     1741 ctgaaggaag ccgccgagcg cgccaagatc gaactttcga ccacgcagtc gaccgaggtg
     1801 aacctgccgt tcatcacggc ggacgccagc ggccccaagc acatgaacat cacgctgacc
     1861 cgcgccaagc tggagcaact ggtcggcgac ctgatccaga agaccatccc gccgatgaag
     1921 cgggcgctgg aagacgccaa gctgaaggcc aaccagatcg acgaagtgtt gctggtcggc
     1981 ggccagacgc gcatgcccaa ggtccaggat gaggtgcgca ccttcttcgg caaggacccg
     2041 catcgcggca tcaacccgga cgaagtggtg gccgtgggcg cggcgatcca ggccggtgtg
     2101 ctgcagggcg aggtcaaggg catcttgctg gtcgacgtga ccccgctgac cctgagcgtc
     2161 gagacgctgg gcggcgtgtc cacgccgatg atcacccgca acacggcgat cccgaccaag
     2221 aagagcgaga cctactcgac ggcgagcgac aaccagccga gcgtggagat ccacgtgctg
     2281 cagggcgagc gcccgatggc ctccgagaac aagagcctgg gccgcttcat cctcgacggg
     2341 attccgcccg cgccgcgcgg cgtgccgcag atcgaagtga ccttcgacat cgacgcgaac
     2401 ggtaccctga acgtgaccgc gcgcgacaag gcgagcggca aggagcagaa gatcaccatc
     2461 acggcttcct ccggcctgag caaggacgaa gtggaccgca tggtgaagga agccgaggcc
     2521 cacgccgagg aagacaagcg ccgccgcgaa ctgatcgagg cgcgcaacca ggccgactcg
     2581 ctgatctact cgaccgagaa gacgctgcag gaatacggcg ataagatctc ggccgacgtg
     2641 aagagcgacg tggagcagaa gctggccgcg ctgcgcgagg cccgcgaagc cgacgacccg
     2701 gcccgcatca cctcggcggt gcaggatctg gcccatgccg cccagaagat cggcgaggcc
     2761 atgtacggcc agccgggcgg tgacggcacg ggcgccgaag cgggcgccgg agccggcgct
     2821 ggagccggcg gctacaaccc gccgagcgac gaaggcaccg tggaaggcga gttccgcgag
     2881 gtctaagcgc catttcggca aacaccgcag ccgccgggga tgcactctgc atccccggcg
     2941 gccctttctt tgcccagcac cgattaaagt ttttgcccga aaactttaat cggcggctcc
     3001 gtcattaaag tttgctttca tccgtgctgg gcgctcgcgt tatgctataa tgcgagcaac
     3061 cctagccgaa agaaacacga tgaacctcga acgcttccaa cagagtcccg caggtcatat
     3121 tgtgcaagct gtccaccggg gtacgccgta tgtggcgttc gtgcccaacc cgctgccgcc
     3181 gcgcctggag ttagatcccg cgctctggcg tagcctctcg gatgccgatc gggcgcttgg
     3241 cgaattggcc gggttaggcc ggaccctgcc caatccccag ttgctcatca acccttttat
     3301 gcgccgtgag gcagtattgt cctcgcgtat cgaaggcacc caggccggga tcgccgacct
     3361 gtatgcctac gaagcaagcc agcttgcgct gccggggttc gacgaaagca cgcctgcctc
     3421 ggatgtgcaa gaggtgctca attacgtcca cgccttggag tacgccctgg aggaggttag
     3481 tcgcctaccc gtcagcctcc gcctgctgcg caatatgcac gcgcgcttga tggaaggggt
     3541 tcgcggggca catgccacgg caggcgcgtt ccggcgtatc cccaatttga tcgggcaccc
     3601 cggcgccacg ttgcagaatg cgcgttacgt gccgccgccg gtcccccaga tgctggacgc
     3661 gctggatgcg ctggagaaat atctgcacgc gcaaagcgag gatgattatc cgccgctcgt
     3721 ccggctggcg ctgatccact accagtttga agcgatccac ccgtttgaag acggcaatgg
     3781 ccggtcaggc cgcctgctca tctccttgct gctggtgaac tggggcctgc tgcccgcgcc
     3841 gctgctgtac ctcagcgcct ttttcgagcg ctaccgggcg acgtattacg acttgctatt
     3901 tgccgtcagc gagcgaggca cctggcgcga gtgggtcttg ttcttcttgc agggcgtctc
     3961 cgaccaggcg gcggacgcca ttgcccgcgc caagcgcttg caagatctgc gcgagggctg
     4021 gcgggcgcaa ttgctgcaag ggcggaccgg cgcgaccgct ttgcacctgc tggatacgct
     4081 gtttgtcgcc ccagttatca cgacccgtgg cgcgcaggat ctcttaggtg taacctaccc
     4141 ggcggcgaag gccaatatcc agaagcttgt gcaggcgggc attctcgtgc ccggcccgcg
     4201 cgcatccaac gggcaaacgt atgtcgccga agctatcttg cagatccttc gagatgagcc
     4261 gtagcagccg ccgattaaag tttttgcccg aaaactttaa tgagtcgccc cgccattaaa
     4321 gtttgcttta atgcaccgcg ggctatgtgc ccccgtcccg cccgatcgcg cgctttagat
     4381 cggcttcagc atcgaatagt tggtgacgcg gtagccctgg cgggcgtaga ggcgctgggc
     4441 cacggcgttg tcgctgaaca cgttgaggcc gatgtgccgg atgcccagcg ggcgcatctc
     4501 ggcttcgagc aagtcgagcg tctgggcgcc gtagcctttg ccccgctggt cggcgttgat
     4561 gtcgatgaaa tagatgaagg ccatgtgctc ggcgtcctcg atgccgaccc agaggtggcc
     4621 gaccaccgcg ccggtctcgc ccgtgaccac ccataaccgc tgccccgcgg tgtgaacccc
     4681 gtctttcagg aaccgggcgt attgatcggc ggcaatggcg cgttcctgct cgatgggcgt
     4741 gccgaaattg cgggcacgct cctgggcgta gtcctccatg ttaggacctt tgaactgctc
     4801 gaactcttcc tcggtcatgg gacgcaatgt aagcattgta gcgaactcct ttttctgata
     4861 ttgatgtccg gtccattcta cggcggatgc gggcgggcgg caagcacccg gcgcccgctt
     4921 tccgcaagcc agcgagtttg ctacaatagg ctcataacca gacgttgacg ctttcctggc
     4981 cgcggcttga gcctggtacg tatctga
//
LOCUS       DASUYC010000368         6845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_66253,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000368 DASUYC010000000
VERSION     DASUYC010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6845
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66253"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VF276_06065"
     CDS             <1..457
                     /locus_tag="VF276_06065"
                     /inference="COORDINATES: protein motif:HMM:NF013038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HEX6606466.1"
                     /translation="VVGRISMDQTTIDVTGIPGVREGDPVVVVGRQGKEQVSFTGLAA
                     LIDTIPYELLTHLNKRLPRVYLQGGEVVQLTTLLGQVDLTLAEPLSGLPEPSGPTYAD
                     LPAIPADLAQSWLDTGDGPALDTPEPAPRPARRKGRVASIRRPPRTPSE"
     gene            514..1815
                     /locus_tag="VF276_06070"
     CDS             514..1815
                     /locus_tag="VF276_06070"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HEX6606467.1"
                     /translation="MRAVDLILAKRNGEAHTREQLEWLIGGMLDGSVPDYQVAAWLMA
                     VCWRGMTPEETAALTSVMVNSGRRLDLSGAAPFVADKHSTGGVGDKTTLVVAPLVAAA
                     GVPIGKMSGRGLGFSGGTIDKLESIPGFRVQLSDAEFVEGVRRVGMVVAAQTSDLAPA
                     DGRLYALRDVTGTVESLPLIASSIMSKKLAAGANAIVLDVKVGQGAFMKTEADARALA
                     EAMVSIGRAAGLQVRAVLSSMDQPLGLAIGNALEVREAVETLRGDGPADLLDLALALG
                     SQLLLMAGKAADAAAARDVLLEGLQSGAARAKLRAFVAYQGGDAGYIDHPEQLPVAPA
                     VVPVPAPDAGYVATIDAEELGLAAVTLGAGREVKGAQIDPAVGFTLRRKIGEAVRPGD
                     SLIDIHAASPAAAAQVAKRVAAAFTISPDPVPVPPLILGIY"
     gene            1830..2516
                     /locus_tag="VF276_06075"
     CDS             1830..2516
                     /locus_tag="VF276_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606468.1"
                     /translation="MLRPAPVPRPGGWLWLGWVAATMLGALGGLLLAALVVPFAVGLP
                     MPGAPALWPAILAGAALLYWGGIGVAQGVALARIVPGARGDIWAAVSLVAAVAGLLLS
                     IAFGWIPAAALADVLFQHPELIGDLLVAWTIYGATVMGLGQALLLRRYLRGGLWWAPA
                     PGLGAVVGLAVLSAGWPGALPGASSDVRLEPLFLTTAVSWAGAAMLSGLVLVVLLRRA
                     PAGPVPASPT"
     gene            2857..3717
                     /gene="rsmI"
                     /locus_tag="VF276_06080"
     CDS             2857..3717
                     /gene="rsmI"
                     /locus_tag="VF276_06080"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX6606469.1"
                     /translation="MGTLHVVSTPIGNLEDLSMRALRVLREVRLIAAEDTRHTRKLLN
                     HFGITTPAISYHAHNERARLEPILAALAEGDVALVSDAGTPGFSDPGLELIRAALAAG
                     HSVTAVPGPVAAVTALILSGLPATRFTYLGFVPRQGTERRALLAQVAAQPWPLVCYEA
                     PHRLVDLLTDLLAVLGNRPCAVARELTKMHEEVLRAPLGEALAHFQATPPRGEFTVVI
                     DGAPPPDAATRAAEADSLEARAAVRLQELVADGLSPRDAVQHVAGELGLNRRKVYNIW
                     LASAPQDPAR"
     gene            3875..4897
                     /locus_tag="VF276_06085"
     CDS             3875..4897
                     /locus_tag="VF276_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase"
                     /protein_id="HEX6606470.1"
                     /translation="MPETQRVPETQRIEEFLAHLAVEKSFSGNTLAAYRNDLTQFLAY
                     LGGDSASPERAAVERGDDPWASVDRADIVDFLLFLKERSYATSTVARKIAAVKSFFHW
                     LAAEKLVPADPTQDLESPRVSKYLPQAISVDNVETLLLQPTLVETPEALRDRAMLEVL
                     YATGMRVSELVALNLDDVIDADEVPPHMNGAAIGPHMAVRCQGKGNKERIIPLDVSAA
                     RQALEDYLARGRPRLVGEQEEDALFLNHRGQRLTRQGFWLILKAYARSAGIEDVTPHT
                     LRHSFAAHKLKEGTALREVQHLLGHASISTTQIYTHVESGSGEGRPSTKRGGVRRPPP
                     PRRASA"
     gene            4977..6536
                     /locus_tag="VF276_06090"
     CDS             4977..6536
                     /locus_tag="VF276_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606471.1"
                     /translation="MIAEPSTDPVAGLADRYPLTHAAFAAMPGGTATLAAVARLEQRW
                     QQASRGQVPLGLLGTVQGPLPPLPPAQVYDVIVAGGGLGLLTGYALARFGRGVLVFDR
                     DAVGCAHREWNISRRELGVLVADGLFTWDELDACVATTYDYGVLSFDATGTGLPATPL
                     RLEGVLDTALDAQALLDLARTRFLAVGGTILEGRRFVRMHTRADGPVESLVELTGPGG
                     APERYRARLVIDMMGSISPIALALNGGRPFDGVCPTVGTVVRGLDVPPGVGDVLVTVA
                     GAQQGRQLIWEGFPGRAGETTVYLFYYDLARPDHAPRQSLLDLFEQYFALLPSYRPLR
                     ADFAHLRPVYGYIPARHGRRGETARRGLICLGDAAAGQSPLTFCGFGSNTRHLPHVSH
                     ELNALLRQDRLEARDLRPIGPHQPNLKPTWTFARLMQPWPGAGPYAVNRLMNAFCAGL
                     QAAGPAVTVRFLQDRHSFADYLRILLTTARRHPEVFAITWRALGPRGLLRWLRDIAAF
                     GGLAMRARLPR"
     gene            6638..>6845
                     /locus_tag="VF276_06095"
     CDS             6638..>6845
                     /locus_tag="VF276_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606472.1"
                     /translation="MSSRSIAHRLLRGLLLLVCLVLPLALTGCLVQTPPEFVSPTATP
                     TAPPPPPVGGRIAFLSDRNDGKREL"
BASE COUNT          956 a         2455 c         2362 g         1072 t
ORIGIN      
        1 ggttgttggg cggatcagca tggatcagac gaccatcgac gtgaccggca tccccggtgt
       61 gcgcgagggc gatcccgtgg tcgtggtggg ccgccagggc aaagagcagg tctccttcac
      121 cgggctggcc gcgctcatcg acaccattcc ctatgagttg ctgacccatc tcaacaaacg
      181 cctgccgcgc gtctacctgc aaggcggcga ggtggtccag ttgacgacgc tgctcggcca
      241 ggtcgatctc acgctggccg aaccgctctc cgggctgccc gaacccagcg gcccgaccta
      301 tgccgacctg cccgcgatcc cggcggacct ggcccaatcc tggctggaca ccggcgatgg
      361 gccggccctc gacacacccg agccagcccc gcgcccggcg cgccgcaaag gccgggtggc
      421 gtcgatccgc cgcccgccgc gcacgccgag cgaataacgc aggcggcacc ggtcgccctt
      481 gatccggcga tactgcacct gggaggagga gacatgcgcg cagtagacct gattctcgcc
      541 aaacgcaacg gcgaggcgca cacccgcgag caactggagt ggctgatcgg cgggatgctc
      601 gacggctcgg tgcccgacta ccaggtggcc gcctggctga tggccgtctg ctggcgcggc
      661 atgacacccg aggaaaccgc cgccctcacc agcgtcatgg tcaactccgg gcggcggctg
      721 gacctcagcg gcgccgcgcc cttcgtggcc gacaagcaca gcaccggtgg cgtgggcgac
      781 aagaccactc ttgttgtcgc gccgctggtg gccgccgcgg gcgtgcccat cggcaagatg
      841 tcggggcggg ggctgggctt ttcgggcggc accatcgata aactggagtc gatccccggc
      901 ttccgggtgc aactgagcga cgccgagttt gtcgaaggcg tgcggcgggt cggcatggtg
      961 gtggccgcgc agacgagcga cctggccccc gccgatggcc gcctctacgc cctgcgcgac
     1021 gtgaccggca cggtcgagag cctgccgctg atcgccagca gcatcatgtc gaagaagctg
     1081 gccgccgggg ccaacgctat cgtgctggac gtgaaggtgg gccagggcgc tttcatgaaa
     1141 accgaagccg acgcccgcgc cctggccgag gcgatggtgt ctatcgggcg ggccgcgggc
     1201 ttgcaggtgc gcgccgtgct gtcgagcatg gatcagcccc tggggcttgc catcggcaac
     1261 gcgctcgaag tccgcgaggc cgtcgagacg ttgcgcggcg acggccccgc cgacctgctc
     1321 gacctggccc tggcgctggg cagccagttg ctgctaatgg cgggcaaggc cgcggacgcc
     1381 gcggcggcgc gggacgtgct gctggagggg ttgcagagcg gcgcggcccg cgccaagctg
     1441 cgcgccttcg tcgcctacca gggcggcgat gccggctata tcgaccaccc cgagcagttg
     1501 ccggtggcgc ccgcggtcgt gccggtgccc gcgccggacg ccgggtacgt cgccactatc
     1561 gacgccgagg aactgggcct ggccgccgtg acgctcggcg cgggccgcga ggtgaagggc
     1621 gcccagatcg accccgccgt cggcttcacg ctgcgccgca agatcggcga ggccgtgcgc
     1681 cccggcgact cgctcatcga catccacgct gcgagtcccg ccgccgccgc gcaggtcgcg
     1741 aagcgcgtgg ccgccgcttt cacgatcagc cccgacccgg tcccggtgcc gccgctgatc
     1801 ctggggatct actgatcgcc gccgggggtg tgctgcgccc tgcgccggtc ccgcgccccg
     1861 gcgggtggct ctggctgggc tgggtggcgg ccacgatgct gggggcgctg ggcggcctgc
     1921 tgctcgccgc gctggtggtg ccgtttgccg tgggcttgcc catgcccggc gcgcccgccc
     1981 tctggcccgc gattctggcg ggcgcggcgc tgctgtactg gggcgggatc ggggtagccc
     2041 agggagttgc cctggcgcgg atcgtccctg gggcgcgcgg cgacatctgg gccgcggtca
     2101 gcctggtggc cgccgtcgcg ggcttgctgc tcagcatcgc cttcggctgg atccccgcgg
     2161 cggcgctggc cgacgtgctg ttccagcacc cggagctgat cggcgatctg ctggtcgcct
     2221 ggaccatcta tggcgccacc gtcatggggc tgggccaggc gctgctgttg cgccgctatc
     2281 tgcgcggcgg cctctggtgg gcgcccgcgc cagggctggg cgccgtcgtc gggctggcgg
     2341 tgctcagtgc gggttggccg ggggcgctgc ccggcgccag ttcggatgtc cggctggaac
     2401 cgctgtttct caccacggcc gtaagctggg ccggggcggc catgctgtcg gggctggtgc
     2461 tggtggtgct gctgcgccgg gcgcctgcgg gcccggtgcc cgcgtctcca acttaagtac
     2521 gagcgcccgc ccccgccgaa ggtcaggtct cgcgattagc cctttggacg gtacaggaaa
     2581 agccagggcg cggtatacta aatcatcacg atgcaccggc ggctggctcc aatgcccccg
     2641 cccggtgcat ttttttttgc ccgctaaggc tgtgcccgct tgcctctacc gcggtcccgc
     2701 atgcgccgct tgttcagcaa ctgcttcatc gcctcccgta taatccctga cggctgacgg
     2761 ctcgtctgct cgacataaaa tcagcggcca aactccccgc ccaaatgatt gacataacgc
     2821 gggaatcctg ctagaataca gaacaaggcg cacactatgg gcacgctgca tgtggtcagc
     2881 acaccgatcg gtaatctaga agatctctcg atgcgggcgc tgcgcgtgct gcgcgaagta
     2941 cggctgattg cggcggagga tacccgccac acccggaagt tgctgaacca tttcggcatc
     3001 acgactccgg cgatcagcta tcacgcccat aacgagcgcg cgcggctgga gccgatcctc
     3061 gccgcgctgg ccgaaggcga tgtcgctctg gtgagcgacg ccggcacacc ggggttttcg
     3121 gaccccggcc tggagttgat tcgggccgcg ctcgccgccg gccacagtgt caccgccgta
     3181 ccgggaccag tcgcagccgt caccgcgctg atcctgagcg gcttaccggc cactcgcttt
     3241 acgtacctgg gcttcgtccc ccggcagggg accgaacgcc gcgcactgct ggcgcaggtc
     3301 gcggcccagc cctggccgct ggtctgttac gaggcgccgc accgcctcgt ggacctgctc
     3361 accgatctac tggcggtgct tgggaaccgc ccgtgcgcgg tggcccgcga gttgaccaaa
     3421 atgcatgaag aggtgctgcg cgccccgctc ggcgaggcgc tggcccactt ccaggccacg
     3481 ccgccccgcg gggagttcac cgtggtgatc gacggcgcgc cgccgcccga cgcggccacc
     3541 cgcgccgccg aggccgatag cctggaagcg cgcgccgcgg tgcggctcca ggaactcgtc
     3601 gcggacggcc tgtcaccccg cgacgccgtc caacacgtgg ccggcgaact cggcttgaac
     3661 cggcgtaaag tctacaacat ctggttggca tcggcccccc aggacccggc ccggtaaccg
     3721 tcgaccgagc cgatcccggc gcgatgccgc cccggttcgt gtgcaccgga ggacaaccct
     3781 tacagcccca gcgttgggcg ctgctccctg gcaggcggcg attcgagctg cggtttgacc
     3841 gttttccccg ccagacgggg agagagaggc ggcaatgccg gagactcagc gagtgccgga
     3901 gacccagcga atcgaagaat tcctggctca tctggcggtc gaaaagagct tttcagggaa
     3961 taccctcgcc gcgtaccgca acgacctgac ccagtttctg gcctaccttg gcggcgacag
     4021 cgcatccccc gaacgggccg ctgtagagcg cggggatgac ccctgggcga gcgtggatcg
     4081 cgccgatatc gtcgatttcc tgctgttcct gaaggaacgc agctacgcga catcgaccgt
     4141 ggcccgcaag atcgcggcgg tgaaatcgtt cttccactgg ttggcggcgg agaaactcgt
     4201 gcccgccgac ccgacccagg acctggagtc gccgcgggtt tccaagtatc tgccgcaggc
     4261 catctcggtg gacaacgtcg agacgctgtt gctgcaacct acgctggtcg agacgccgga
     4321 ggcgttgcgc gaccgcgcca tgctcgaagt gctctacgcc acgggcatgc gcgtcagcga
     4381 actggtggca ctgaacctgg acgacgtgat cgacgccgac gaggtgccgc cccatatgaa
     4441 cggcgcggcg atcgggccgc atatggccgt acgttgccag ggcaagggta acaaagagcg
     4501 cattatcccg ctggatgtca gcgcggcgcg ccaggcgctg gaggactacc tggcgcgcgg
     4561 ccggccccgc ctggtgggcg agcaggagga ggacgcgctc ttcctcaacc accgcgggca
     4621 gcgcctgacg cgccagggct tctggctcat cctgaaagcc tacgcccgct ccgccgggat
     4681 cgaggacgtg accccgcaca cgctgcgcca ctcgttcgcc gcgcacaagc tcaaagaggg
     4741 cacggccctg cgcgaggtgc agcacttact cggccacgcc agcatctcga ccacgcagat
     4801 ctacacccat gtggagagcg gcagcggcga gggccggccc agcaccaagc gcggcggcgt
     4861 gcgccgcccg cccccgcccc gccgcgccag cgcctgaccc ggcgccccgg caatccgcga
     4921 gagaatggcc cgccgtttat ccggcgtggc ctttcttttt agctgggagc gtaccgatga
     4981 tcgccgaacc ctccaccgac cccgttgccg gactcgcaga tcgctatccc ctgacccacg
     5041 ccgctttcgc ggccatgccg ggcggcacgg cgacgctggc ggcggtggcg cgcctggaac
     5101 agcgctggca gcaggcgtcg cgcgggcagg tgccgctggg cctgctgggc acggtgcaag
     5161 ggccgttgcc cccgctgccg cccgcccagg tttatgacgt aatcgtggcc gggggcgggc
     5221 tgggcctgct caccgggtat gccctggccc ggttcggccg gggcgtgctc gtcttcgacc
     5281 gcgatgccgt gggctgcgcc catcgcgagt ggaatatctc gcgccgcgag ttgggcgtgc
     5341 tggtcgcgga cggcctgttc acctgggacg agctggacgc ctgcgtcgcc acgacctatg
     5401 attacggggt gctgtcgttt gacgctacgg gcaccgggtt gccggccacg cctctgcgcc
     5461 tggagggcgt gctcgatacg gcgctcgacg cccaggcgct gctcgacctg gcccgcactc
     5521 gcttcctcgc cgtcgggggc acgatcctgg agggccggcg cttcgtgcgg atgcacaccc
     5581 gcgccgatgg cccggtggag agcctggtag aattgaccgg ccccggcggc gcgcccgaac
     5641 gctaccgcgc ccggctcgtg atcgatatga tgggctcgat ctcgcccatc gctctcgcgc
     5701 tgaacggcgg ccggcccttc gacggcgtct gcccgacggt ggggacggtc gtgcgcgggc
     5761 tcgacgtgcc gccgggcgtg ggcgatgtgc tggtcacggt ggccggcgcg cagcagggcc
     5821 ggcagttgat ctgggaaggc ttccccggtc gcgccgggga aaccaccgtc tatctgttct
     5881 attacgacct ggccaggccg gaccacgcgc cgcgccaatc gctgctggac ctcttcgagc
     5941 agtatttcgc cctgctgccc agctaccggc ccctgcgggc cgacttcgcc cacctgcgcc
     6001 cggtctacgg ctacatcccg gcgcgccatg ggcgccgggg cgagacggcc cggcgcgggc
     6061 tgatctgcct gggcgacgcg gcggcgggcc aatccccgct caccttctgc ggtttcggct
     6121 cgaacacgcg ccacctgccg cacgtctcgc acgaactcaa cgcgctgctg cggcaggacc
     6181 ggctggaagc ccgcgatctt cggcccatcg gcccgcatca gcctaacctg aaacccacct
     6241 ggacctttgc ccggctgatg cagccctggc cgggggccgg gccctatgcc gtcaaccggc
     6301 tgatgaacgc cttctgtgcc gggttgcagg ccgccggccc ggcggtgaca gtgcgcttct
     6361 tgcaggaccg ccacagcttc gccgactacc tgcgcatcct gctgacgacg gcccggcgcc
     6421 acccggaggt gttcgctatc acctggcgcg cactcggccc gcgcggcctc ctgcgctggc
     6481 tgcgcgacat cgccgctttc ggcgggctgg ctatgcgggc acggctgccc aggtagccac
     6541 ctggacttgg tgttgacgcc ccggcgcgtc gcctcgtata atggcgaaga ccgccctatt
     6601 gccggtacgc catggaaaag gaatctcgcc tccgctcatg tcatcgcgct cgatagccca
     6661 ccgcctgctt cggggcctgt tactcctggt gtgcctggtg ctgcccctgg cgctgaccgg
     6721 ctgcctggtc cagaccccac ccgagttcgt cagcccgacc gcgaccccca cggcgccgcc
     6781 gcccccgccg gtaggcggcc ggatcgcctt cctgtccgac cgcaacgacg gcaagcgcga
     6841 actct
//
LOCUS       DASUYC010000369         6746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_86054,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000369 DASUYC010000000
VERSION     DASUYC010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6746
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86054"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="VF276_06100"
     CDS             <1..936
                     /locus_tag="VF276_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010541716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX6606473.1"
                     /translation="SGRNMHFGVREHGMGAVANGMTLSGLRAYTGTFLIFSDYMRPPM
                     RLAALMGINPIFVFTHDSVGLGEDGPTHQPIEMLAALRAVPNSCVIRPADANETAAAW
                     RAALERQDGPSLLVLTRQKLPIFAESARPYEEGLSRGAYVLRDAEGGSPDVILIGTGS
                     EVQLAMGAADQLKQQGVRARVVSMPSWELFERQSQEYRDSVLPPNVKKRVSVEAASTF
                     GWCRWVGDEGHAVGIDHYGASAPYEIIMEKFGLTAGNVAQWALAILGRPAHPAGQGEH
                     PGGHHGRGDAPHERDLEQQQGGTETSTESPAHKQS"
     gene            954..3872
                     /locus_tag="VF276_06105"
     CDS             954..3872
                     /locus_tag="VF276_06105"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HEX6606474.1"
                     /translation="MGKTALNRLYEEQGQAPWLDNISRGLITSGDLRKMIDEDGIVGV
                     TSNPTIFEKAISSGTDYDEQMRDVFGRGQDAGQVFFELMLKDIQDAADIFRGVYDKTN
                     HLDGYISIEVTPDLARDTEKSIEQARMFRERLQRPNIFVKVPATAEGIPAIEQLIYEG
                     ININITLIFAIDRYREVIEAYIKGLERRLAEGKSVDDIWSVASFFVSRVDTNVDKKLD
                     ALIAKAGSEEEKNKLKALKGKAAIANAKIAYQVFQEVFGSERWQKLAAAGAHVQRPLW
                     ASTSTKNPEYRDVLYVEELIGPDTVDTMPPATIIAFNDHGVVKRTVDQDVAGAHRVIS
                     DLNSIGISMDQVTKELEDEGVASFAKSYETLGDAIAAKRDALLAGQQQNAQTDVGEYK
                     TDVEKTLASLQDAGSHIWNKDGNWWTDDPAEAAHIVDRLGWLDVVDRMIGEADNLRAF
                     ADEVRRARFDKVLLMGMGGSSLAPDLFSMEFVTARGFPELYVLDSTDPAVVALCRQEL
                     DPKTTLYIVSSKSGTTTEPHAFMEYFYDRVKQVKGDKVGENFVAITDPGSPLIAEAQK
                     RGFRKIYQNPPDIGGRYSALSYFGLVPAALMGVDVRKLLERAHLMAERCGKGVPAAQN
                     PGVWLGAVLGTLAQQGRDKVTFVTTLGTDSFADWAEQLIAESTGKQGKGLVPVAREEL
                     GTPDVYGDDRVFIYMPKEGDEDKAEYDERIAALMDAGQPVITLPLQDEYDLAGEFFRW
                     EIAVAVAGKVLGINPFDEPNVTESKNNTKRLLEEYGRKKRLPEETPTADEGAVELFAY
                     QADGGKNGTGGQGGDLAGYLQDLFAQVQPHDYVAILAYLEPSANLYDEDGNVEQYGNM
                     ERLETIRTLIRDELHVATSLGFGPRFLHSTGQLHKGGPNNGVFLVFTSDEAADVAIPA
                     LGYSFGTLIRAQALGDIEALVTKGRRVIRLHLTNTDDGLEQAADLVDDALQSGVGVQG
                     "
     gene            3935..4915
                     /gene="gnd"
                     /locus_tag="VF276_06110"
     CDS             3935..4915
                     /gene="gnd"
                     /locus_tag="VF276_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEX6606475.1"
                     /translation="MDIGFMGLGRMGENMVRRLLRGGHRVVAWNRSKGKIDEIAKEGA
                     VAAYTPADLVKELQPRRVIWLMVPAGQPVDDLIEELVPMLDKGDILIDGGNSNFRDSQ
                     RRHAALTAKGFDFLDIGTSGGIWGLQVGYCMMIGGDDDAFNYIEPLLKTLAPEDGYMH
                     TGPSGSGHFTKMVHNGIEYGMLQAYGEGFEIIKASEFPVDLAALSQVWQHGSVIRSWL
                     LDLAVRAFEKEGDLRSIRGYVEDSGEGRWTVQEAINESVPAPVITASLLARFASRQQE
                     SFSAKVVAALRNEFGGHAVQLESGGSSGVSFSPDAPRSSSVVGGQSGRGK"
     gene            4994..6553
                     /gene="zwf"
                     /locus_tag="VF276_06115"
     CDS             4994..6553
                     /gene="zwf"
                     /locus_tag="VF276_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX6606476.1"
                     /translation="MQRPTTTNPLRAGLRQQLTPEPCTIVIFGATGDLTQRKLLPALY
                     NLALDGLLPGAFNIVGVARRPKTDEQFRKEMLTGINEFSRNRPVQPQVWETFSQGIFY
                     VQSEFHDEAGFRRLKEKLDQVDEERGTGHNVLYYMATPPDAYPLIAANLGKVGMVSRE
                     STRDPERDKGWSRLVVEKPFGRDLRSARELNRQLHAVFSERQIYRIDHYLGKETVQNL
                     LVFRFGNIIFEPIWNHRYIDNVQITVAENIGIEGRASYYDNAGAIRDMVQNHMMQTLC
                     LVAMEPPVAFDADAVRDEKVKVLRGIHPLSREQVQTRTVRAQYSAGAINGQPVPGYCQ
                     EPGVNPDSVTETYVALKLFVENWRWAGVPFYLRTGKRLPKRSTEIALQFKQVPHLLFR
                     HSMDGLDANSLVVHIQPDEGTTIKVASKIPGPLMRVRPVNMDFRYGTSFGIEPADAYE
                     RLLLDALLGDSTLFTRRDEVEQAWEIVNSIIDGWQEQAPVDIPKYEAGSWGPEEADRL
                     IARDGRVWRRL"
BASE COUNT         1279 a         2137 c         2257 g         1073 t
ORIGIN      
        1 agcgggcgca atatgcactt cggcgtccgc gagcacggca tgggcgccgt cgccaacggc
       61 atgaccctgt cgggcttgcg cgcctatacc ggcacattcc tgatcttctc cgactatatg
      121 cgcccgccga tgcgcctggc cgccctgatg ggcatcaacc cgatcttcgt cttcacgcac
      181 gacagcgtcg gcctgggcga ggacggaccg acgcatcagc ctatcgagat gctggcggcg
      241 ttgcgcgcgg tgcccaattc gtgcgtgatc cgccccgccg acgccaacga gacggcggcc
      301 gcctggcgcg ccgcgctgga acggcaagac ggcccctcgc tgctcgtgtt gacccggcag
      361 aagttgccga tcttcgccga gagcgcccgg ccttacgagg agggcctgag ccgcggcgcc
      421 tacgtcttgc gcgacgccga gggcggcagc cccgatgtga tccttatcgg caccggctcg
      481 gaagtccaac tggcgatggg cgccgccgat cagctcaaac agcaaggcgt gcgcgctcgc
      541 gtggtcagca tgccgtcctg ggagttgttc gagcgccaga gccaggagta ccgcgactcg
      601 gtgttgccgc cgaacgtcaa gaagcgcgtg tccgtggaag cggcttcgac cttcggctgg
      661 tgccgctggg tcggcgacga aggtcacgcc gtcggcatcg accactacgg cgcctcggcc
      721 ccttacgaga ttatcatgga gaagttcggc ctgacggccg gcaacgtggc ccaatgggcg
      781 ctggcgatcc ttggtcgccc ggcgcatccg gcgggccagg gcgaacaccc cggcggccac
      841 catggccgtg gcgacgcacc gcacgagcgc gacctggaac agcagcaggg cggcacggaa
      901 accagtacgg aaagccctgc tcataagcag agctagcaaa aggagacacg acgatgggta
      961 agactgcttt aaaccgcctg tacgaggagc agggccaggc gccgtggctg gataacatca
     1021 gccgcgggct gatcacctcc ggcgatttgc gcaagatgat cgacgaggat gggatcgtcg
     1081 gcgtgaccag caatcccacc attttcgaga aggccatcag cagcggcacc gattacgatg
     1141 agcagatgcg cgacgtgttt ggccgtggtc aggacgccgg gcaggtgttc ttcgagctga
     1201 tgctgaagga cattcaggat gccgccgata tcttccgcgg tgtgtacgac aagacgaacc
     1261 atctcgacgg ctatatcagc atcgaggtaa cgccggatct ggcgcgcgac acagagaaga
     1321 gtatcgagca ggcgcgcatg ttccgcgaga ggctgcagcg gcccaatatt ttcgttaagg
     1381 tgccggccac cgccgagggc atcccggcca tcgagcagtt gatctatgaa ggcatcaaca
     1441 tcaacatcac gctgatcttc gccatcgatc gctaccgcga agtcatcgag gcctacatca
     1501 agggcctgga gcggcggctg gctgaaggca agtcggtgga cgacatctgg tcggtggcgt
     1561 cgttcttcgt cagccgggtg gataccaacg tcgataagaa actcgacgcg ctgatcgcca
     1621 aggccggctc ggaggaggag aagaacaagc tcaaggcgct caagggcaag gcggccattg
     1681 ccaacgccaa gatcgcctac caggtcttcc aggaagtctt cggcagcgag cgctggcaga
     1741 aactggccgc ggcgggcgcc cacgtgcagc gcccgctgtg ggcttcgacc agcaccaaga
     1801 atcccgagta ccgcgatgtg ctctacgtcg aggagttgat cggccccgac accgtggaca
     1861 ccatgccgcc cgccaccatc atcgccttca acgaccacgg cgtcgtgaag cgcaccgtgg
     1921 accaggatgt ggccggcgcg caccgggtga tctcggatct caattctatc ggcatctcaa
     1981 tggaccaggt gaccaaagaa ctggaagacg agggtgtggc gagcttcgcc aagtcttatg
     2041 aaacgctggg cgacgccatt gccgccaagc gcgacgcgct cctggccggc cagcagcaga
     2101 acgcgcagac cgacgtgggc gaatataaga ccgacgtgga gaagacgctg gcgagtctgc
     2161 aagacgcggg gtcccacata tggaataaag acggcaactg gtggacggac gaccccgccg
     2221 aggccgctca cattgtggac cgcctcggct ggctcgacgt ggtcgatcgg atgatcggcg
     2281 aggccgataa cttgcgcgcc tttgcggacg aagtccgccg ggcccgcttc gataaggtgc
     2341 tgttgatggg catgggcggc agcagcctgg cgcccgacct gttcagcatg gaatttgtga
     2401 cggcgcgcgg cttcccggag ctgtacgtgc tcgacagcac ggaccccgcc gtggtcgcgc
     2461 tctgccgcca ggagttggac ccgaagacga cgctctacat cgtgtccagc aagtcgggca
     2521 cgacgacgga gccgcacgcg ttcatggagt acttctatga tcgcgtcaag caggtgaaag
     2581 gcgataaggt gggcgagaac ttcgtcgcca tcaccgatcc gggctcgccg ctgattgccg
     2641 aggcgcaaaa gcgcggcttc cgcaagatct accagaaccc gcccgacatc ggcgggcgct
     2701 actcggccct gtcctacttc ggcctggtgc cggcggcact gatgggcgtc gatgtgcgca
     2761 agctgctgga acgcgcccac ctgatggccg agcgctgcgg caagggggtg cccgccgccc
     2821 agaaccccgg cgtctggctc ggcgctgtcc tgggcaccct ggcccagcag ggccgggaca
     2881 aggtgacgtt cgtcaccacg ctcggtactg atagctttgc cgactgggcc gagcagttga
     2941 tcgccgagag caccggcaag cagggcaagg gtctggtgcc cgtggcccgc gaggaactcg
     3001 gcacgcccga cgtgtacggc gacgaccgcg tgttcatcta tatgccaaag gaaggcgacg
     3061 aggataaggc cgaatacgac gagcgcatcg cggcgttgat ggatgccggg caaccggtca
     3121 taacgctgcc cttgcaggac gagtacgacc tggccggcga gttcttccgc tgggagatag
     3181 ccgtcgccgt ggccggcaaa gtactcggca tcaatccctt cgacgagccg aacgtcaccg
     3241 agagcaaaaa caacacgaag cgcttgctgg aggagtacgg ccgcaagaag cggctgccgg
     3301 aagaaacgcc gaccgccgac gaaggcgccg tcgagttgtt cgcgtaccag gcggacggcg
     3361 gcaagaacgg taccgggggg cagggcggcg atctcgcggg ctatctgcag gacctgttcg
     3421 cccaggtcca gccgcacgac tacgttgcca tcctggcgta cctggagccg tcggccaacc
     3481 tctatgacga ggacggcaac gtcgagcaat acggcaatat ggaacggctg gagacgatcc
     3541 gcaccttgat ccgcgacgag ctacacgtgg cgacgtcgct gggctttggc ccgcgcttcc
     3601 tgcactccac ggggcagttg cacaagggcg ggccgaacaa cggcgtcttc ctcgtcttta
     3661 cctcggacga ggccgccgac gtggcgatcc cggcgctggg ctatagcttc ggcacgctga
     3721 tccgcgccca ggcgctgggc gacatcgagg cgctggtcac caagggccgg cgcgtgatcc
     3781 ggctgcacct gacaaacacc gatgatgggc tggaacaggc cgccgatctg gtcgacgatg
     3841 cgctgcagag cggcgtgggc gtccagggct aggccgggcc gttgaccggg gccggcgccc
     3901 accgcgccgg ctccccacca ccacaggagg atgtatggac atcggtttta tgggcctcgg
     3961 ccgcatgggc gagaacatgg ttcgccggct tttgcgcggc gggcatcgcg tcgtcgcctg
     4021 gaatcgcagc aaagggaaaa tcgatgaaat cgccaaagag ggcgccgtcg cggcatacac
     4081 gcccgccgac ctggtcaagg aattgcagcc ccgccgcgtg atctggctca tggtccccgc
     4141 cgggcagccg gtcgacgacc tgatcgagga actggtgccc atgctcgaca agggcgacat
     4201 cctgatcgac ggcggcaact ccaacttccg cgactcgcaa cgccggcacg ccgcgctcac
     4261 cgccaagggt ttcgatttcc tcgacatcgg cacctccggc ggcatctggg ggctgcaggt
     4321 cggctactgt atgatgatcg gcggcgacga tgatgctttc aactatatcg agccgctgct
     4381 gaagaccctg gcgcccgaag acggctatat gcataccggc ccgagcgggt cgggccactt
     4441 caccaagatg gtccataacg gcatcgagta cggcatgctc caggcgtacg gcgagggctt
     4501 cgagatcatc aaggcgtcgg agttcccggt ggacctggcg gcgctgtccc aggtctggca
     4561 gcacggcagc gtgatccgct cctggctgct cgatctggcg gtgcgcgcct tcgagaaaga
     4621 gggcgacctg cgctccatcc gcggctacgt cgaagactcg ggcgaaggcc gctggacggt
     4681 gcaagaagcg atcaatgaaa gcgtgccggc gccggtcatt accgcgtcgc tgctggcgcg
     4741 cttcgcctcg cggcagcagg agtccttcag cgcgaaggtg gtggcggccc tgcgcaacga
     4801 gttcggcggc catgctgtgc agctcgaaag cggcggatcc agcggggtca gcttctcgcc
     4861 ggatgcgccg cggagttcgt ccgtggtcgg gggccagagc gggcgcggca agtaagtagt
     4921 cggctatccg ccggctgggg cgcgggcgcc gcatgtggcc ccgtgccccg gcctcaacct
     4981 ggatgtgaca gagatgcaaa gaccgaccac cacgaacccg ctgcgcgcgg ggttgcggca
     5041 gcaactcacg cccgagccgt gcaccatcgt gatcttcggg gcgaccgggg atctgacgca
     5101 gcgcaagctc ttgcccgctc tctacaacct ggctctggac gggttgctgc cgggtgcgtt
     5161 caacatcgtg ggggtggcgc gccgcccgaa gaccgacgag cagttccgca aggagatgct
     5221 gaccgggatc aacgagtttt cgcgcaaccg tcccgtgcaa ccccaggtct gggagacctt
     5281 ctcccagggc attttctatg tccagagcga gttccacgac gaggccgggt tccggcgact
     5341 caaggaaaag ctggaccagg tggatgagga acggggcacc gggcacaacg tgctctacta
     5401 catggcgacc ccgcccgatg cctatccgct gattgcggcc aacctgggca aggtgggcat
     5461 ggtctcgcgc gagtcgacgc gcgatcccga gcgcgacaag gggtggagcc gcctggtggt
     5521 ggagaagccg tttggccggg atctgcgcag cgcccgcgag ttgaaccgcc agttgcacgc
     5581 ggtcttcagt gagcgccaga tctaccgcat cgaccactat ctaggcaagg agaccgtgca
     5641 gaacctgctg gtcttccgct tcggcaacat cattttcgag ccgatctgga accaccgcta
     5701 catcgacaac gtgcagatca ccgtggccga gaatatcggc atcgagggcc gggcgtcgta
     5761 ctatgacaac gccggggcga tccgcgatat ggtgcagaac catatgatgc agacgctatg
     5821 cctggtcgcg atggagccgc cggtcgcctt cgacgccgac gccgtgcgcg acgaaaaggt
     5881 gaaggtgttg cgcggcatcc atccgctgag ccgcgagcag gtgcagacgc gcacggtgcg
     5941 cgcgcagtac agcgccgggg ccattaacgg ccagccggtg cccggctact gccaggaacc
     6001 gggggtcaac cccgattccg tcaccgaaac ctatgtagcc ctgaagctgt tcgtcgagaa
     6061 ctggcgctgg gcgggcgtgc cgttctatct acgcaccggt aagcgcttgc ccaagcgcag
     6121 taccgagatc gccctgcagt tcaagcaggt gccacacttg ctcttccgcc acagcatgga
     6181 tggcctcgac gcgaattcgc tggtcgtcca tatccagccg gatgaaggca cgacgatcaa
     6241 ggtggcgtcg aagattcccg gccccctgat gcgcgtgcgg ccggtgaaca tggatttccg
     6301 ctatggcacc tcgttcggca tcgaacccgc cgacgcctac gagcgcctgc tgctggatgc
     6361 cctgctcggc gactccacgc tcttcacacg ccgtgatgaa gtcgagcaag cgtgggagat
     6421 cgtgaattcg atcattgatg gctggcagga gcaggcgccc gtcgatatcc cgaagtacga
     6481 ggccgggagc tgggggccgg aggaggctga ccgcttgatc gcccgcgacg ggcgcgtctg
     6541 gcgccggcta tagagtcgcg ccgcctcccg aacaaccgcg ggctgccgca gcgcgccggc
     6601 tgccgcgctg tcccggcatg gcatataata ggaatagcga accgttgttc gcgcggcagt
     6661 agagacggcg cggcttgcat cctacgcatg ccctagatgg gagactgacc atggacgacg
     6721 acgagaagcg gggtccggta ccccag
//
LOCUS       DASUYC010000370         2834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1619396, whole genome shotgun sequence.
ACCESSION   DASUYC010000370 DASUYC010000000
VERSION     DASUYC010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1619396"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="VF276_06120"
     CDS             <1..846
                     /locus_tag="VF276_06120"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX6606477.1"
                     /translation="YVKQHYSFEAEAVEQKVMRGQSDAILLAEPLIDKAEDLFVVFSD
                     TLFEADLGAVQHLGDADGAAFVKEVPDPSRFGIAVSNAQGYVTRMVEKPQEPLSHDAV
                     VGIYYFKRAGDLYDAIHEQVERKIMLKNEYFLADAIQVMVEHGKRLKVLPVTVWEDTG
                     TRDAILHSNRYLLRKQHGDQPDTPYVQGSALIVPPVFIGEGVTLEQCVIGPYASVGAG
                     TSIRESIVRDCIIGEQAQVRATICTATLIGDRGVVEGSFQTLNIGDDSALGVDPSGAI
                     DETFK"
     gene            847..1575
                     /locus_tag="VF276_06125"
     CDS             847..1575
                     /locus_tag="VF276_06125"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6606478.1"
                     /translation="MTAPARNGQLGVLLLHGYTSSLDTVNGLVPTLTRLGLPYRMPVL
                     RGHGTHQRDLRGVTAADWYADAAAALDDLLREVPRAAVVGLSMGGLVATQLGIERAND
                     LTGVALVAPFFGTKNPLAPLTGLIGRVMPYFPMPRSIYDPEVYARNTNYKQGEATSFA
                     SAYRYSRTMRARIGELRLPLLIVASRRDQVIPVPVIEDVVARAGTPPDQREVYWLERS
                     GHDILMDMERETAYNLIGAWLARL"
     gene            1651..2568
                     /gene="prmA"
                     /locus_tag="VF276_06130"
     CDS             1651..2568
                     /gene="prmA"
                     /locus_tag="VF276_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX6606479.1"
                     /translation="MEWVELAVDVDREAVEATSELFALYGYNQGVVVEEAIIPQPDED
                     YVVDLTAPVQVRTYIVADERAEDIRQRIAEALWHLGRLRHVSELRATRLKEEDWANAW
                     KAHYQTQRIGKHMIIKPSWLEYTPQPGDHVIELDPGMAFGTGTHPTTRLCLRAIEDCA
                     TPAMRRILDLGTGSGILAIGVARMLPEAAIWALDTDPVAVETSAANVAANGLRERITV
                     EQGSLVPGVEPDIHFDLIIANILARVIIDLAPAIARALVPGGLAITSGIIDERGADVD
                     AALRKAGLRILDTRQEGDWLAIIVRRPAE"
BASE COUNT          480 a          983 c          949 g          422 t
ORIGIN      
        1 tatgtcaagc agcactattc cttcgaggcc gaggccgtcg agcagaaagt gatgcgcggg
       61 cagtcggacg ccatcttgct ggccgagccg ctgatcgaca aggccgaaga cctcttcgtc
      121 gtcttctccg acaccctgtt cgaggccgac ctgggcgccg tgcagcacct gggcgacgcc
      181 gacggcgcgg cgttcgtcaa agaggtgccc gaccccagcc gcttcggcat cgccgtgagc
      241 aacgcgcagg gctacgtcac gcgcatggtc gagaagcccc aggagccgct gtcgcacgac
      301 gccgtcgtcg gtatctatta cttcaagcgg gccggcgacc tctacgacgc gatccacgag
      361 caggtcgagc gcaagatcat gctgaagaac gagtatttcc tggccgacgc gatccaggtc
      421 atggtcgagc acggcaagcg gctcaaggtg ctgccggtga cggtctggga agacaccggc
      481 acgcgtgacg ccatcctgca cagcaaccgc tacctgctgc gcaagcaaca cggcgaccag
      541 cccgacacac cctacgtgca gggcagcgcg ctgatcgtcc cgcccgtgtt tatcggcgag
      601 ggcgtgacgc tggagcaatg cgtgatcggc ccctatgcca gcgtcggcgc ggggaccagc
      661 atccgcgagt ccatcgtgcg cgactgcatc atcggcgagc aggcgcaggt gcgggccacg
      721 atctgcacgg cgacgctgat cggcgaccgg ggcgtggtcg agggctcctt ccagacgctg
      781 aatatcggcg atgactccgc gctcggtgtc gatccgagcg gcgccatcga cgaaaccttc
      841 aagtaggtga cggccccggc gcgcaacgga caactcggcg tgctcttgct gcacggctac
      901 acgtcgagcc tggacacggt gaacggcctg gtgccgacgc tgacccggct gggcctgccc
      961 taccgcatgc ccgtcttgcg cggccacggc acgcaccagc gcgacctgcg tggcgtgacg
     1021 gcggccgact ggtacgccga cgccgcggcg gcgctggacg acctgctgcg cgaggtgccg
     1081 cgggctgccg tggttggcct ctcgatgggc gggctggtcg ccacgcagtt gggcatcgag
     1141 cgcgcgaatg acctgaccgg ggtggcgctg gtggccccct tcttcggcac caaaaacccg
     1201 ctcgcgcccc tcactgggtt gataggtcgg gtcatgccct acttccccat gccccggtcg
     1261 atctacgatc ccgaggtata cgcccgcaat accaactaca agcagggcga agccacgtcc
     1321 ttcgcctcgg cctatcgcta cagccggacc atgcgcgccc gcatcggcga gttgcgcctg
     1381 ccgctgctga tcgtcgccag ccgccgcgac caggtcatcc ccgtgccggt catcgaagac
     1441 gtggtggccc gcgcgggcac gccgcccgac cagcgcgagg tctactggct cgaacggtcg
     1501 gggcacgaca tcctcatgga catggaacgc gaaaccgcgt ataacttaat cggcgcctgg
     1561 ctggcccggt tataggccgg cggggcggca ggtgcgccgg cccggccgcg cgggtgccgg
     1621 gcggacgatg tgcataagga ggatgcccgg atggagtggg tggaactggc cgtggatgtc
     1681 gaccgggaag ccgtcgaggc taccagcgaa ctcttcgccc tgtacggcta caaccagggc
     1741 gtggtggtcg aggaagcgat tatcccccag cccgacgagg actatgtcgt ggacctcacg
     1801 gcacccgtgc aggtgcgtac ctacatcgtc gccgacgagc gggccgagga catccgccag
     1861 cgcatcgccg aggcgctctg gcacctgggc cgcctgcgtc acgtgagcga actgcgcgcc
     1921 acccgcctga aggaagaaga ctgggccaac gcctggaagg cccactacca gacccagcgc
     1981 atcggcaagc acatgatcat caagccgtcg tggctggagt acacaccgca gcccggcgac
     2041 cacgtgatcg aactcgaccc cggtatggcc ttcggcaccg gcacccaccc caccacgcgc
     2101 ctctgcctgc gcgccatcga agactgcgcc acgcccgcca tgcgccgcat cctcgacctg
     2161 ggcacggggt cgggcatcct ggcgatcggg gtggcgcgca tgctgccgga ggccgcgatc
     2221 tgggcgctgg acaccgaccc tgtcgccgtg gagacctccg ccgccaacgt ggcggcgaac
     2281 gggctgcgcg agcgcatcac cgtggagcag ggctcgctgg tgcccggcgt ggaacccgac
     2341 atccacttcg acctgatcat cgccaatatc ctggcccgcg tgatcatcga cctggccccg
     2401 gccatcgcgc gggcgctggt gccgggcggc ctcgccatca cttccggcat catcgacgag
     2461 cgcggcgcgg acgtggacgc cgccctgcgc aaagccggcc tccgcatcct cgacacccgc
     2521 caggaaggcg actggctggc gatcatcgtc cgccggcccg ctgagtagga cgagtggtca
     2581 gtggtcggtg gtcagtggtc agtgacgtgc ctgcggcacc gctgcgcgaa cagatgtaga
     2641 gatcgcgagt ggatcaggcc aggatagcat agccatgcaa cgcttcttcg tcgatcccac
     2701 cctccccttg cagccggact ccgagattgc gctgcccacc gcgctaacgc accaggtggg
     2761 ccatgtgctg cggatgcggc cgggcgaccg gatcgtgctg ctggacaaca gcggcgacga
     2821 gtgcgaggtc gagt
//
LOCUS       DASUYC010000371         2328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_244901,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000371 DASUYC010000000
VERSION     DASUYC010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244901"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VF276_06135"
     CDS             <1..634
                     /locus_tag="VF276_06135"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX6606480.1"
                     /translation="LLQRRAALEGRADPRDARTLRILVQQTLRLHRLVVSLLDLSRID
                     TGQLSIERQVVDLNRLLDRLLDEMSPAFERHTLLARLGEAPVLVLGDELRLEQVLYNL
                     LQNAVKYSPNGGTIGVRLDCEAGAAVVTVTDEGVGVPAGAEDQLFDRFYRADTPATQR
                     ISGMGVGLYVVKEIVTLHGGTVAAAGRDGQGSVFTVRLPLYQPPAPGAEI"
     gene            718..>2328
                     /locus_tag="VF276_06140"
     CDS             718..>2328
                     /locus_tag="VF276_06140"
                     /inference="COORDINATES: protein motif:HMM:NF039926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5939 domain-containing protein"
                     /protein_id="HEX6606481.1"
                     /translation="MSRPLRLVRRMILDMPAEAFFDAATMTDRVERSTGLPKARFQAT
                     RDPEGRQALRADAKLLGLPVHWYEHPFEWVHNRYFSVHRIFEGVPLKSIDVTTTVRPL
                     GPKRSAVELRAEVVPSNILGRIGAFFVFGVYTLTRVSRLYRTFAERYQRRAGTVFPPP
                     LPPDLHKRALEQRARDLRALKLPPGLADRLIHHLATAPDDEVTNMRPFVFAARWGIER
                     MQALRLFLYATQTGLLDLHWDVLCPNCRVAKAQVTNLSDLELTAHCDTCNIRYDVNFD
                     EYVELRFTVSGAVREVLDASFCIGGPYQTRHIVAQFRLALGEARTLPPVLPPGAYRWR
                     TRQRTERGIFHIDPAGATGPAEYRFTADGVTAPDAEPRLGPQAELRFENAGPDELLLI
                     LENADWGNEGVSAALVTSLQEFRNMFSSQVLAPGLGVSVKNLTVLFSDLKDSTVMYER
                     FGDSPAYARVRDHFVILTDAITEHNGALVKTIGDAVMAVFRVPADAVAACLAIQEQMA
                     GRNQAMPPETQLLVKLGLHSGPCIAINAN"
BASE COUNT          360 a          801 c          767 g          400 t
ORIGIN      
        1 gttgctgcag cggcgggccg cgctggaggg ccgcgccgac cctcgcgatg cgcgaaccct
       61 gcgcattctt gtgcagcaga cgctgcgcct gcatcgcctg gtggtttcgc tgctggatct
      121 ctcccgtatc gacaccggcc agcttagcat cgagcgccag gtggtcgacc tgaaccgcct
      181 gctcgaccgc ttgctggacg agatgagtcc cgcgttcgag cgccataccc tcctggcccg
      241 cctgggcgag gcccccgtgc tggtcctggg cgatgagctg cggctggagc aggtgttgta
      301 taatctgttg caaaacgctg tcaagtacag ccccaacgga gggaccatcg gcgtgcgcct
      361 cgattgcgag gccggcgcgg ctgtggtcac cgtcaccgac gaaggagtgg gcgtcccggc
      421 gggtgccgag gatcagctct tcgaccgctt ttaccgcgcc gataccccgg cgacgcaacg
      481 aatcagcggc atgggcgtgg ggctgtatgt ggtgaaagag atcgtaaccc tgcacggcgg
      541 tacggtggct gccgccggcc gcgacgggca aggcagcgtg tttaccgtgc gcctccctct
      601 gtatcagccc ccagcgccgg gcgcggagat ctagcccccg gactcgtgcg tgattcagtt
      661 atcggcctca gtgcaaccgc ccgcctctgc gcggctggga gggagaagaa ttcgcgtatg
      721 tcccgaccat tacggctcgt gcgccggatg atcctcgata tgcctgcgga ggctttcttc
      781 gatgcagcca ccatgactga tcgcgtggag cgcagcaccg gtttgcccaa ggcccgcttc
      841 caggcgacgc gcgatcccga gggccgccag gccttacgcg ccgacgcgaa gctgctgggt
      901 ctcccggtcc actggtacga gcatccgttt gaatgggtcc ataaccgcta tttcagcgtg
      961 catcggattt ttgaaggtgt gccgctgaag agcatcgacg tgacgacgac ggtgcgaccc
     1021 ctcggcccga agcgcagcgc cgtcgagcta cgggccgagg tggtgcccag caacatcctg
     1081 gggcggatcg gcgccttctt cgtcttcggc gtctacacgc tgacgcgcgt cagccgtctg
     1141 taccgcacct tcgccgagcg ctaccagcgc cgggccggca ccgtattccc accgccgttg
     1201 ccgcccgatc tgcacaaacg cgcgttggag cagcgtgccc gcgatctgcg cgcgctgaag
     1261 ctgccgccgg ggctggccga ccggctcatc caccacctgg cgacggcgcc cgacgacgaa
     1321 gtgaccaaca tgcgcccctt cgtgttcgcc gctcgttggg gcatcgagcg gatgcaggcg
     1381 ctgcgcctct ttctgtacgc cacgcagacg gggctgctgg acctgcactg ggacgtgctc
     1441 tgccccaact gccgtgtggc gaaggcccag gtgaccaatc tatccgacct ggagctcacg
     1501 gcccactgcg acacctgcaa tatccgctac gatgtgaact tcgacgagta tgtcgagttg
     1561 cgattcacgg tgagcggcgc cgtgcgcgag gtactggacg ccagcttctg catcggcggg
     1621 ccgtaccaga cgcggcatat cgtcgcccag ttccgcctgg ctctcggcga ggcgcgcacg
     1681 ctgccgccgg tgctgccgcc cggcgcctac cgctggcgca cccggcagcg caccgagcgg
     1741 gggatcttcc acatcgatcc ggccggtgcc accggcccgg cagagtatcg cttcacggcg
     1801 gacggcgtca ccgcgcccga tgccgagcca cgcctggggc cgcaggccga gttgcgcttt
     1861 gaaaacgccg gcccggacga gctgctcttg atcctggaga atgccgactg gggcaacgag
     1921 ggcgtgagcg ccgcgctggt cacgtcgttg caggaattcc gcaacatgtt ctcctcgcag
     1981 gtgctggcgc cgggtctcgg cgtgtccgtc aagaacctga ccgtgctgtt cagcgacctc
     2041 aaggactcga cggtgatgta cgagcgcttc ggcgactccc cggcctacgc ccgcgtgcgc
     2101 gaccatttcg tgatcctcac cgatgcgatc accgagcaca acggcgccct ggtcaagacc
     2161 atcggcgacg cggtgatggc cgtgttccgc gtgcccgccg acgcggtggc cgcctgcctg
     2221 gcgatccagg agcagatggc cgggcgcaac caggccatgc cgcccgagac gcagttgctg
     2281 gtcaagctgg gtctgcatag cggcccgtgc atcgccatca acgccaac
//
LOCUS       DASUYC010000372         3935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_676858,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000372 DASUYC010000000
VERSION     DASUYC010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676858"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VF276_06145"
     CDS             complement(<1..617)
                     /locus_tag="VF276_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606482.1"
                     /translation="MQHTQFRLNYTEMEQAQAGLSDRFALLALFPAHYSFPPEEAGAL
                     WGIDTAGAAGSLDLLQQRRLVERGPGRDPMYAMPDVVRRLATEYLASHPDLSPPGQER
                     LITYYAAMAAANGEGLPAHIAAITDVYDALSAALTWAEQQQRWAEVSVMARALARFFY
                     MRRMTNQYRSILMRGIYAAQQTRRLDEQAWYLREQGSLAADSGDF"
     gene            complement(621..1376)
                     /gene="rsmG"
                     /locus_tag="VF276_06150"
     CDS             complement(621..1376)
                     /gene="rsmG"
                     /locus_tag="VF276_06150"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEX6606483.1"
                     /translation="MDVLAAGATALGLPLTAGQLARFAAYAGLMREWNTRANLTAITD
                     PALVQTRHFVDSLAVGAALLAQVGGTAGSALPAIFAGGRGLIDLGTGAGFPGLPLKIL
                     WPDLPVTLVDSIGKKTAFLLAAAEALGLRDVTVLTGRAETLGQDRAHRAAYDVATARA
                     VAALPVLAEYGLPLCRVGGIMFAPKKGDMDAEIAQVERALPKLGGATVSRYRYTLPGE
                     PDERLVVGMPKVAPTPSGYPRRVGLPDKRPLGI"
     gene            complement(1397..2440)
                     /gene="fni"
                     /locus_tag="VF276_06155"
     CDS             complement(1397..2440)
                     /gene="fni"
                     /locus_tag="VF276_06155"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEX6606484.1"
                     /translation="MSIEQRKRDHIRICLEEDVQGEGITSGFGGYRFLHQALPEIDLD
                     AVDCSLELWGRRLGAPLLISSMTGGTGPAEAINRHLAEAAETLGLAIGVGSQRAAILD
                     PALAPTYQIRRTAPHALIFANIGAVQLNYGFGLDECRRAVEMVEADALILHLNPLQEA
                     VQAEGDTRFGGLAAHIAEICAAFARDGVPVIAKEVGWGIAPETARRLWDAGVTAIDVA
                     GAGGTSWSQVEAHRAPNDALRRVAEAFRSWGIPTTESLRMVREAGVPEGRHIFASGGL
                     RSGVDLAKAVALGATLGGIARPFLEAGAVSAEATVEAGRRLITELRISMFCIGAGDLP
                     ALRATPHLYEVAS"
     gene            complement(2534..>3935)
                     /locus_tag="VF276_06160"
     CDS             complement(2534..>3935)
                     /locus_tag="VF276_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606485.1"
                     /translation="VRWRVAGGRRLALAAGGWLLLAVASKLTAVVLMPLFVVYALSSP
                     AGRRYRALLRRREALAAPRYAVAVGIVAVLLGGAVFLGVRAKFDDLHGHYDLVGRVLA
                     GRQDLSYAARALYGLTLSPGKGLLPFAPPVLAGLIGMWFLARTRRAEALLCGAAGGLF
                     VLIYSFDTGWHGGACWGPRYLLPVLPLLMLPFGALASRVWAARRTGRGQVALALLGVL
                     LLAGGVVQVAALAVDPIQYYLDVLRRHPLTLETGPDYLQEIHFDPALSPIPGHLALAA
                     QNLETLAAGRTNFYPVPYEPEAYWKYFQYLAAPDSAWVHLAGWIEAGDYRAATNAPPD
                     AVIVADPGAAGGRAVQGPPGGAALLLTTPPAQFPSGPYQVWARLRMGAADPAQAVVRL
                     TARSKEAVLATREVAAGALAAGQDYQETGLDVVLDYAHPVWFEVQATGVTTVTLDGLR
                     LQPSGPLSPLPLAAQK"
BASE COUNT          595 a         1421 c         1392 g          527 t
ORIGIN      
        1 tcgaagtcgc ccgaatcggc ggccagcgat ccctgttcgc gcagatacca ggcttgctcg
       61 tcgagccgcc gggtctgctg ggccgcgtag atgccgcgca tcaggatgct gcgatactgg
      121 ttggtcatgc ggcgcatgta gaagaagcgc gccagcgccc gcgccatcac cgacacctcg
      181 gcccagcgct gctgctgctc ggcccaggtc agcgcggcgc tgagggcgtc gtagacatcg
      241 gtaatggcgg cgatgtgggc cggcaagcct tcaccattgg cggcggccat ggcggcgtag
      301 taggtgatga gccgctcctg gcccggcggg ctgagatccg gatggctcgc caggtactcg
      361 gtggctagcc ggcgcaccac gtcgggcatg gcgtacatgg ggtcgcgacc ggggccgcgc
      421 tccacgagcc gccgctgttg caacaggtcg aggctgcctg ccgcgcccgc ggtgtcgatg
      481 ccccagagcg cccccgcctc ttcgggcggg aaactgtagt gcgccgggaa cagggccagc
      541 agcgcgaagc ggtcgctcag gccggcctgg gcctgctcca tctcggtgta gttgaggcga
      601 aactgggtgt gctgcatagc tcagatcccc aatgggcgct tgtccggcag cccgacccgg
      661 cgcggatagc ccgacggcgt gggcgcgact ttcggcatgc ccaccaccag gcgctcgtcc
      721 ggttcgccgg gcaacgtata gcggtagcgc gagaccgttg cgccccccag cttgggcaaa
      781 gcccgttcca cctgggcgat ctcggcgtcc atatcgccct tcttgggggc gaacatgatg
      841 ccgcccacgc ggcagagcgg cagaccgtat tcggccagca ccggcaaggc ggccacggcg
      901 cgcgccgtgg cgacatcgta ggccgcgcgg tgggcgcggt cctggcccaa cgtctcggcg
      961 cgcccggtca gcacggtcac gtcgcgcagg cccaacgctt ccgccgccgc cagcaagaag
     1021 gccgtcttct tgccgatcga gtcgaccagc gtgaccggca ggtcgggcca gaggatcttc
     1081 aggggcaggc cgggaaagcc ggccccggtg cccaggtcga tcaggccccg gcccccggca
     1141 aaaatagcgg gcagcgcgct cccggcggtg ccgcccacct gcgccagcag cgccgcgccc
     1201 acggccagcg agtcgacgaa atgccgcgtc tgcaccagcg ccgggtcggt gatcgcggtc
     1261 aggttggcgc gggtgttcca ctcgcgcatc aggccggcgt aggcggcgaa gcgcgccagt
     1321 tgcccggcgg tcagcggcaa gccgagcgcc gtggcgcccg cggccagcac atccatcgag
     1381 gccgcctccc gcgcggtcag gatgccacct cgtagagatg gggcgtggcg cgcaaggcgg
     1441 gcagatcgcc ggcgccgatg cagaacatgc tgatgcgcaa ctcggtgatg agccggcggc
     1501 ccgcctcgac ggtcgcctct gcggagacgg cccccgcttc caggaagggc cgggcgatgc
     1561 cgcccagcgt cgcgcccagg gccacggcct tcgccagatc gacgccgctg cgcagcccgc
     1621 cgctggcgaa gatgtgccgc ccctccggca cgccggcctc gcgcaccatg cgcaggctct
     1681 cggtggtggg gatgccccaa ctccggaagg cttcggccac gcggcgcagg gcgtcgttag
     1741 gggcgcgatg ggcctcgacc tggctccagg acgtgccccc ggccccggcc acgtcgatgg
     1801 ccgtcacccc ggcatcccag agccgccgcg ccgtctccgg ggcgatgccc cagcccactt
     1861 ccttggcgat aaccggcacg ccgtcgcgag cgaacgccgc gcagatctcc gcgatgtgcg
     1921 ccgccagtcc gccgaagcgg gtgtcgcctt cggcttgcac ggcttcttgc agcgggttga
     1981 ggtgcaggat gagcgcgtcg gcctcgacca tctccacggc ccgccggcac tcgtccagcc
     2041 cgaagccgta gttgagttgc accgcgccga tgttggcgaa gatgagcgcg tggggcgccg
     2101 tgcggcggat ctggtaggtg ggcgccaggg ccgggtccag gatggccgcg cgctgcgagc
     2161 cgacgccgat cgccaggccc agcgtctcgg cggcctcggc caggtgccgg ttgatggcct
     2221 ccgccgggcc ggtgccgccg gtcatggaac tgatcagcag gggcgcgccc aggcgccggc
     2281 cccacaactc taggctgcaa tcgaccgcgt ccaggtcaat ctcgggcaac gcctggtgca
     2341 gaaagcgata gcctccgaag ccggaggtga tgccctcgcc ctgcacatct tcttccaggc
     2401 agatgcggat atggtcgcgc ttacgttgtt cgatagacat gcagagtggt cgctgttcct
     2461 tacctttgat aaggttgttg gggtcattat agcatagtgt atagacgaag gtacatagcc
     2521 caaatttcct accttatttt tgcgccgcca agggcagcgg ggaaagcggc ccgctcggtt
     2581 gtagccgcag gccgtcgagc gtgaccgtgg tcacgccggt ggcctggacc tcgaaccaga
     2641 ccgggtgcgc gtagtcgagc accacgtcca gcccggtctc ctgatagtcc tggcccgctg
     2701 ccagcgcgcc cgccgcgacc tcgcgcgtcg ccagcacggc ctctttgctg cgcgcggtga
     2761 gccggaccac ggcctgcgcc ggatcggcgg cgcccatccg caaccgcgcc cagacctggt
     2821 agggcccgct ggggaactgc gccgggggcg tggtcagcaa aagtgcggcc ccgcccggcg
     2881 ggccctgcac ggcgcgcccg cccgccgcgc caggatcggc cacgatcact gcatctggcg
     2941 gcgcattggt ggcggcgcgg tagtcgcccg cctcgatcca gcccgcgagg tggacccagg
     3001 cgctgtccgg cgcggcgagg tattggaagt acttccagta ggcttcgggc tcatagggca
     3061 cggggtagaa gttggtgcgc ccggcggcca gcgtctccag gttctgcgcg gccagcgcta
     3121 ggtggccggg gataggagag agcgccgggt cgaagtggat ctcttgcagg tagtccggcc
     3181 cggtctccag cgtcagcggg tggcggcgca gcacgtcgag gtagtactgg atagggtcca
     3241 cggcgagggc cgccacctgg accacgcccc cggccagcag cagcacgccg agcagggcca
     3301 acgccacctg cccgcgcccc gtgcgccgcg ccgcccagac gcgcgaggcc agcgcgccga
     3361 agggcagcat cagtagcggc aacacgggca gcaggtagcg cgggccccag caggcgccgc
     3421 cgtgccagcc cgtgtcgaag ctgtagatca gcacgaacag cccgcccgcc gccccgcaca
     3481 gcagcgcctc cgctcgccgc gtgcgcgcca ggaaccacat cccgatcaga ccggcgagca
     3541 ccggcggcgc gaagggcagc aggcccttgc ccggactaag cgtgaggccg tagagcgcgc
     3601 gggcggcata gctcaggtct tgccgcccgg ccagcacgcg gcccaccagg tcgtagtgcc
     3661 catgcaggtc gtcgaacttg gcccgcacac ccaggaacac cgctccgccc agcagcaccg
     3721 ccacgatgcc cacggcgacg gcatagcgcg gggcggcgag cgcttcgcgc cgccgcaaca
     3781 gcgcccggta gcgccggccc gccgggctgc tgagggcata gaccacgaaa agcggcatca
     3841 gcaccaccgc cgtcagcttg gacgcgacgg ccagcagcag ccagccccca gcggccagcg
     3901 ccaagcgccg cccgcccgct acccgccagc gcacc
//
LOCUS       DASUYC010000373         3914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1111401, whole genome shotgun sequence.
ACCESSION   DASUYC010000373 DASUYC010000000
VERSION     DASUYC010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111401"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="VF276_06165"
     CDS             <1..689
                     /locus_tag="VF276_06165"
                     /inference="COORDINATES: protein motif:HMM:NF012410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606486.1"
                     /translation="GLEVLYFVEPIDNFVAVSLTEFAGKKLRNADDPDISLPPLDEAA
                     QAAQPEPLSGADFAPLLLRVREVLGDRVLEVREGKTLTASPCRLVSPPGADRDLQRVR
                     RYLDQQYEVPKKILELNRSSPLIQGLAARVQANPADPTVDLIIEQLYDSALLQEGLHP
                     NPAGMVGRIQQLMEAAVGGAGARSAPARAAAPPQPEAAAPAPEPDTAEAPPSEAATPQ
                     VVIGEVITED"
     gene            complement(777..1832)
                     /locus_tag="VF276_06170"
     CDS             complement(777..1832)
                     /locus_tag="VF276_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606487.1"
                     /translation="MTNGKRINPLSRRPRRQRLVVAVRGAAGSGKSVFASTLADAGLG
                     RLCYFDVERKARLLPGSDGTRFDAIEVQHPDELPEFIDWALEGEGRDQNYGCYVLDSW
                     AMYFGRKHRETVQAVRERTGDPLAQPSADELAADQMIFQEVLRRLCIDSGACVVVTDQ
                     IGAKGLEEREENELGRVLPLTSGGLEYFVDVMVEASVRVVDFETERVFRVIKTNSPAF
                     PIGMEFANPTFADFLAQLGEGAVLAAPVAEPDVPELLDEPTPPALLRPAGPTLDDLRA
                     LAQEHGLREADLVVAARHYCNGKSDLTRLTGDEIADLHHRLTARIEKQAAAAPAPVEE
                     APAQVAANGARPRQRRA"
     gene            2537..3778
                     /locus_tag="VF276_06175"
     CDS             2537..3778
                     /locus_tag="VF276_06175"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6606488.1"
                     /translation="MARNPLKLDRDEQRVDPHRGGALDAATEAALAHARQLTAQGRYR
                     DALAVCRRLRAGAPAHSDILGLLGWLYAQTGSLKQGLENLAEALVIGPRSPIVLYHLA
                     QVYLQAGAPVHALHSLRGYWTDVQALPVALDLPDPQPLMAALVIFMREEEGFPNVNEE
                     FQDRAMMAAERGRLRLLFNDDPAQALGELREAIRLAPKYPQPRNNLSLAYFYNGQADR
                     AVATLETTLREVSPNNVYALATLAFIRWMTGGAVADSLALLERAAAALEPQSRLLDRT
                     RVAEVAGLLGAHALAYRVLAPEGETALQPAPAEEGQAGAARNRLLATALANLDRADEA
                     AALLRESPALPGDPLAQRLLTAIDTGETLPALAGGGYPYVGWHDLLSLHLLRRLAGTP
                     ATSRGPFRAHAPPDAAQVPLQ"
BASE COUNT          589 a         1384 c         1335 g          606 t
ORIGIN      
        1 gcggcctgga agtgctctac ttcgtcgagc ccatcgacaa cttcgtggcg gtcagcctga
       61 ccgagttcgc cggcaagaag ctgcgcaacg ccgacgaccc cgatatcagc ctgccgccgc
      121 tggacgaggc ggcccaggcc gcccagccgg agccgctgtc cggcgccgac ttcgcgccgc
      181 tgctgctgcg cgtgcgcgag gtattgggcg accgcgtgct ggaggtgcgc gagggcaaga
      241 cgctcaccgc cagcccctgc cggctggtct cgccgcccgg cgccgaccgc gatctccagc
      301 gcgtgcgccg ctacctggac cagcagtacg aggtgcccaa gaagatcctg gagctgaacc
      361 gcagcagccc gctgatccag ggcctggccg cgcgcgtgca ggccaatccc gccgatccca
      421 ccgtggacct gatcatcgag caactctacg acagcgcgct gttgcaagaa gggctgcacc
      481 ccaaccccgc gggcatggtg ggccgcatcc agcaactgat ggaagccgca gtcggcggcg
      541 cgggcgcccg ctccgcgccc gcccgcgccg ccgcgccccc ccagccggag gccgccgccc
      601 cggcgccgga accggacaca gcggaggcac ctccgtctga ggccgccacg ccgcaagtcg
      661 tcatcggcga ggtgatcacc gaggattgac cgtcaggtcc ggcatagacg aaggcggcag
      721 ctagagcgtg ctagctgccg ccggtgtttg tgcggttggg tgggtcggga ggtggcttag
      781 gcgcggcgct ggcgcgggcg ggcgccgttg gccgcgacct gggccggggc ttcctcgacc
      841 ggggcggggg ccgcggcggc ctgcttctcg atgcgcgccg tcaggcggtg gtgcaggtcc
      901 gcgatctcgt cgccggtcag gcgggtgagg tcgctcttgc cgttgcagta gtggcgcgcg
      961 gccaccacca ggtcggcttc gcgcagaccg tgctcctggg ccagggcgcg cagatcatcc
     1021 agggtcggcc cggcggggcg cagcagcgcc ggcggggtcg gctcgtccag cagttcgggc
     1081 acgtcgggct cggcgacggg ggcggccagc accgcgcctt cgccaagctg ggccaggaag
     1141 tcggcgaacg tcggattggc gaactccatg ccgatgggga aggccgggct gttggtcttg
     1201 atcacgcgga acacgcgctc ggtctcgaag tcgaccacgc ggacgctggc ctcgaccatc
     1261 acgtccacga agtactccag accaccgctc gtcagcggca gcacgcggcc cagttcgttc
     1321 tcctcgcgct cttccagccc cttggccccg atctgatcgg tgacgacgac gcaggcgccg
     1381 ctgtcgatgc agaggcggcg cagcacttcc tggaagatca tctggtcggc cgccagttcg
     1441 tcggcgctgg gctgggccag cgggtcgccg gtgcgctcgc gcacggcctg caccgtctcg
     1501 cggtgcttgc ggccaaagta catggcccag gagtcgagca cgtagcagcc gtagttctgg
     1561 tcgcggcctt cgccttcgag cgcccagtcg atgaactcag gcaactcgtc ggggtgctgc
     1621 acctcgatgg cgtcgaagcg ggtgccgtcg ctgccgggca acagccgggc cttgcgctcc
     1681 acgtcgaagt agcagaggcg gcccagcccg gcgtcggcca gggtggaggc gaacacgctc
     1741 ttgccgctgc ccgccgcccc acggacggcg accaccaggc gctgccggcg cgggcgccgg
     1801 ctgagggggt tgatccgttt tccgtttgtc atatgtctgt gcggcgtgcg tgccgggcct
     1861 gcgacccgcg cctgctcgac cgccctcctt tcaaggtcta cacctgggcc aactgggagg
     1921 ggcgtgtcac agcgccgctc atcgcccggc ccttttgccg ataaatccgc cactgccttg
     1981 ccggatgcag tggcccacat cgcatagtat accacaaccc gcacaaatgt tctagaggcc
     2041 ggcgccccca atttgccgga atccgcgctt gcaaattcta tggccgtgag gtgggcaatt
     2101 gcggaaaccg ggtcacggac gcatcgcgcc ccgttgccgg gcgcgcgccc tgcctgcccg
     2161 gatccgccta tttagcggcg gaatagtcgt ctatctcccc atccgtctac cattgccggg
     2221 cattgagcgt gcgtctactg cctttgtgct ttagagggca gcggaaaccg ccctggccta
     2281 tcatccaaca ccttgtgatt gtaggggccg gtttcaaacc ggcctgatcg ctgatcagaa
     2341 ttgcggcggt ggactacttt accatctcca tagttggcgc aacagcggaa gaagaacgtt
     2401 aagaactgtg taatcgcctg ttgaacatgt aaaatggcga tcaattggac ggatgcactt
     2461 gacgaaaccg catatcgcta gtacaatgta tttcaattac cggcgcggac taactcatgc
     2521 gtggggcgat gatctgatgg cgcggaatcc cctgaaacta gatcgcgacg agcagcgggt
     2581 cgatccgcat cgagggggtg ccctcgacgc cgcgaccgaa gcggcgctgg cccatgcccg
     2641 gcagttgacg gcgcaggggc gctaccggga tgccctggcc gtgtgccggc ggttgcgcgc
     2701 cggagcgccc gcccattccg acatcctggg cctgctcggc tggctctacg cccagaccgg
     2761 gagcctgaag cagggcctcg aaaatctggc cgaggcactg gtgatcgggc cgcgcagccc
     2821 catcgtcctc taccacctgg cgcaagtcta tttgcaggcc ggcgcgcccg tgcacgcgct
     2881 gcacagcctg cgcggctact ggactgatgt gcaggccctg cctgtcgccc tcgacctgcc
     2941 cgatccccag ccgttgatgg ccgccctggt catcttcatg cgcgaggaag agggctttcc
     3001 caacgtgaac gaggagttcc aggaccgggc gatgatggcc gccgaacgcg ggcgcctgcg
     3061 cctgctgttt aacgacgacc cggcgcaggc actgggcgaa ctgcgcgagg ccattcgcct
     3121 ggcgcccaag tatccccagc cgcgcaacaa cctgagcctg gcctatttct acaacggcca
     3181 ggccgaccgg gccgtcgcca ccctggagac caccctgcgc gaggtcagcc cgaacaatgt
     3241 ctacgccctg gccaccctgg ccttcatccg ctggatgacg ggcggcgcgg tcgccgactc
     3301 gctggcgctg ctcgaacgcg ccgccgcggc cctggagccg cagagccgcc tgctggatcg
     3361 cacccgcgtg gccgaagtgg cggggttgct cggcgcgcac gcgctggcct atcgcgtgct
     3421 cgcgcccgaa ggggagacgg cgctgcaacc ggcgcccgcc gaagaaggcc aggccggcgc
     3481 ggcgcgcaat cgcctgctgg cgaccgccct ggcgaacctc gaccgcgccg atgaggccgc
     3541 cgcgctgctg cgcgagtcgc ccgcgctgcc cggcgacccg ctggcgcagc gcctgctcac
     3601 ggccatcgac accggcgaga ccttgccggc cctggccggc ggcggctatc cctacgtggg
     3661 ctggcacgac ctgctgtcgc tgcatctgct gcgccgcctg gccggcacgc cggcaacctc
     3721 gcgtggcccg ttccgcgccc acgcgccgcc cgatgccgcg caggtgcccc tccagtagat
     3781 cctcggcggg cggcgtgccg gcgagcgcca gcaaggtcag ggcgagttgc tccaggagcg
     3841 gttcgcccat ccagatgagc agggcgcacg cgagcgtcag gcgcgggtaa accgtcacga
     3901 tctggcgcgc gatc
//
LOCUS       DASUYC010000374         3087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_232436,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000374 DASUYC010000000
VERSION     DASUYC010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232436"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2130
                     /locus_tag="VF276_06180"
     CDS             <1..2130
                     /locus_tag="VF276_06180"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEX6606489.1"
                     /translation="GNEELGLTNTFDSILAANSATGHTYLFSSGDHGSHQPFGTRDPA
                     PQYPASSRYVLSIGGTHFSANIGSTWPGEAAWIYQASGPGGLPVGSGGGYSELYNRPS
                     WQVAPGFNNTRRGYPDVAAVGDPNTGMFTCYGDTTPTCAQYGGTSLSAPLWAGMLAIT
                     DQYLVAQGRARTGFVNPLLYALSANAGPNPPYHDITSGTNGAYNAGAGWDAVTGLGTP
                     DLWNLAQALLTLPAGTPTPTQTPPPVPPTNTPPGVVPTATPPPVPPTSTPPAATATAC
                     PAGQFSDVPPGSTFYTYITDLVNKGAISGYSDCTFRPSATITRGQVAKVIVLAFGFQL
                     VTPAQPHFSDVPPGSAFYSYIETAYARGIISGYADGTFRWSNNVTRGQLTKMVSLGRS
                     WRILTPAQPTFRDVPPTETFYSYIETTYAHGVISGYDCGTGCLEFRSSATSTRGQGAK
                     IIDLSIYAADNTATPTTAPPTVPPTNTPPPGPTNTPAPTDTPAPTSTSTPVPPTATQT
                     PTNTPSPTSTPTVRPTDTPQPAPYLTGFQPNSVEQYDTETQISMTGNWFGMTQGAGFV
                     VVNGRVVTVDTWDNTLIRFRITTAVNPATPATVRVVRDDGQSATSTAFSVTPRQHPYI
                     FSYSPSAIVHGDGMTVVTASGIQFGTTPGTVTLLGTYPATIVSWSNTQIQFRVDPNTP
                     AANPISVIVNSPQNGSYTEFGRFAIFP"
     gene            2250..>3087
                     /locus_tag="VF276_06185"
     CDS             2250..>3087
                     /locus_tag="VF276_06185"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase family protein"
                     /protein_id="HEX6606490.1"
                     /translation="MNADSPSAAPETMRAIQEFVRALPKAELHVHLEGSVRPRTLLEL
                     AGRHRVNLGVGDEAGLRARYQFTDFPAFVSTYVTVCDCLRSGEDFALITQELGHDAAR
                     QNIHYLEATFTAVRHVQATGLTFDEVMDGIAAGAAAAQREYGVIMRFIPDYPRDFGVD
                     GLMQTVEWAIAGMDRGVVALGLSGMENEVPSLPFAPAVAWAKAAGLYFAPHAGEIAGP
                     QVVRDCLAMGADRIGHGIHAADDPALLDELRALQIPLEVCPTSNLRTGAATSLADHPL
                     RRL"
BASE COUNT          518 a         1229 c          917 g          423 t
ORIGIN      
        1 ggcaacgaag agttgggcct gaccaacacc ttcgactcga ttctcgccgc caacagcgcc
       61 accgggcaca cctatctctt ctccagcggc gaccacggct cgcaccagcc gttcgggacc
      121 cgcgacccgg cgccgcagta ccccgcgtcg agccgctacg tgctgtccat cggtggcacc
      181 cacttcagcg ccaacatcgg cagcacgtgg ccgggcgagg cggcatggat ctaccaggcg
      241 tccggccccg gtgggctgcc cgtgggcagc ggcggcggct actccgaact ctataaccgc
      301 ccgtcgtggc aggtggcccc cggcttcaac aacacgcggc gcggctatcc cgacgtggcc
      361 gctgtgggcg acccgaacac cggcatgttc acctgctatg gcgacaccac acccacctgt
      421 gcgcaatatg gcggcaccag cctctcggcg ccgctgtggg ccggcatgct ggcgatcacc
      481 gaccagtatc tggtggcgca gggccgggcg cgcaccggct tcgtgaatcc gctgctgtac
      541 gcgctgtccg ccaacgccgg ccccaacccg ccctaccacg acatcaccag cggcaccaac
      601 ggcgcctaca acgccggcgc gggctgggac gccgtgaccg gcctgggcac gcccgacctc
      661 tggaatctgg cgcaggcgct gctcaccctg cccgcgggca ccccgacgcc cacgcagacc
      721 ccgccgcccg tgccgcccac aaacacgccg cccggcgtag tgcccacggc cacgccgccg
      781 cccgtgcctc cgacgagcac gccgcccgcg gccaccgcca cggcctgccc ggcgggccag
      841 ttcagcgacg tgccgcccgg ctcgaccttc tacacctaca tcaccgacct ggtgaacaag
      901 ggcgccatct ccggttacag cgactgcacc ttccggccca gcgccacgat cacccgcggc
      961 caggtcgcca aggtgatcgt gctggccttc ggtttccaac tggtgacgcc cgcccagccg
     1021 cacttcagcg atgtgccgcc cggctcggcg ttctattcgt acatcgagac ggcctatgcc
     1081 cgcggcatca tctccggcta cgccgacggg accttccgct ggtccaacaa cgtcacgcgc
     1141 ggccagttga ccaagatggt ctcgctgggc cgcagctggc gcatcctgac gcccgcccag
     1201 ccgaccttcc gcgacgtgcc tccgacagag accttctaca gctacatcga gaccacctat
     1261 gcccacggcg tgatcagcgg ctacgactgc ggcaccgggt gcctggagtt ccgctcatcg
     1321 gccacgtcca cccgcgggca gggcgccaag atcatcgacc tgtcgatcta cgcggcggac
     1381 aacacggcca cgccgaccac cgccccgccg acggtcccgc cgacgaacac gccgccgccc
     1441 ggcccgacca acacgcccgc gccgaccgac acaccggcgc cgaccagcac gtccactccc
     1501 gtgccgccga cggccacgca gacgccgacc aacacgccgt cgccgaccag cacgccgacc
     1561 gtgcgcccga ccgacacgcc gcagcccgcg ccctacctca ccggcttcca gccgaacagc
     1621 gttgagcagt acgacaccga gacgcagatc tcgatgacgg gcaactggtt cggcatgact
     1681 cagggcgccg ggttcgtcgt ggtcaacggt cgcgtcgtca ccgtcgatac ctgggacaac
     1741 acgctgatcc gcttccgcat caccacggcg gtgaacccgg ccaccccggc caccgtgcgc
     1801 gtggtccgcg acgacggcca gtcggccacc agcaccgcct tcagtgtgac gccacggcag
     1861 cacccctaca tcttcagcta ctcgccgagc gccatcgtcc acggcgacgg catgacggtg
     1921 gtgacggcca gcggcatcca gttcggcacc acgcccggca cggtgacgct gctcggcacc
     1981 tacccggcca ccatcgtcag ttggagcaac acccagatcc agttccgggt cgatcccaac
     2041 acgcccgccg ccaacccgat cagcgtcatc gtcaactcgc cgcagaacgg cagctacacc
     2101 gagtttggcc ggttcgctat cttcccctga cgatagcgcc ccacgcaacg caatgcacga
     2161 gagcctggcg catggcgcca ggctcttgtc atgctgtgct acaatcagac caacaacctg
     2221 aaagccaacc cgaggggaga caccaccaca tgaatgcaga ctcgccatcc gctgcacccg
     2281 aaaccatgcg cgccatccag gagtttgttc gcgccttgcc caaggctgaa ctccatgtcc
     2341 acctggaagg ctcggtgcgc ccgcgcaccc tgctggaact ggccgggcgc caccgcgtca
     2401 acctgggcgt gggcgacgaa gccgggctgc gcgcccgcta ccagttcacc gacttcccgg
     2461 ccttcgtgag cacctacgtc accgtctgcg actgcctgcg cagcggcgag gacttcgccc
     2521 tgatcaccca ggagttgggc cacgacgcgg cccggcaaaa tatccactac ctggaagcga
     2581 cgtttaccgc cgtgcgccat gtccaggcca ccgggctgac cttcgacgag gtgatggacg
     2641 gcatcgccgc gggcgccgcc gccgcgcagc gcgagtacgg cgtgatcatg cgcttcatcc
     2701 ccgactaccc gcgcgatttc ggcgtggacg gcctgatgca gacagtggaa tgggccatcg
     2761 ccgggatgga ccgcggggtc gtggccctcg ggctgagcgg catggagaac gaggtgccct
     2821 cgctgccctt cgcgcccgcc gtggcgtggg ccaaggccgc cggtctctac ttcgcgcccc
     2881 acgccgggga gatcgccggg ccgcaggtgg tccgcgactg cctcgccatg ggcgccgacc
     2941 gcatcggcca cggcatccac gccgccgacg acccggccct gctggatgaa ctgcgcgcct
     3001 tgcagattcc attagaggtc tgcccgacca gcaacctgcg tacgggcgcc gcgacttcgc
     3061 tggccgatca cccgctgcgc cgcctct
//
LOCUS       DASUYC010000375         3015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1476862, whole genome shotgun sequence.
ACCESSION   DASUYC010000375 DASUYC010000000
VERSION     DASUYC010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1476862"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="VF276_06190"
     CDS             complement(<1..724)
                     /locus_tag="VF276_06190"
                     /inference="COORDINATES: protein motif:HMM:NF020028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEX6606491.1"
                     /translation="MTKTLPTRAEIPEQYTWDLSTIYPTNAAWEEDFAALPALLDEFT
                     GYQGRLAADGKTLLAAMTLQDTLGRRLDRLAIYAFLRRDEDTANSTYQSLADRVQQLG
                     VRAHAATAFVRPELLALPDGTLDRLMDEEPGLRVYAHDFEDLLRGKPHVLPQAEEQLL
                     AQVGELAAAPSTVFSMLNNADLTFGTVTDEEGSETQLTLGRYQRFMRANNRALRADAF
                     LKLHGAYSAHRNSIAALYSANVK"
     gene            1018..>3015
                     /locus_tag="VF276_06195"
     CDS             1018..>3015
                     /locus_tag="VF276_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606492.1"
                     /translation="MNDPHEPAAVLTVAATNRAREAASGSGAAPLASAPERGARRRDA
                     AVAGLLALAGALLYIPTAPFYFRPDDDGHAIFDALLVRQGLRPYLDFFVHDSPFIHYW
                     NALLLQVGGVDMLTLRYGLVPFKILLLPAIYLLARPVAGRLVAALAAAMVLMSDSAVN
                     FGMPHPGWYAMALALLGLLGMRAWLGSGRGAWLVGAGLAFGVAVDVKQNLGLFLLGAG
                     GLFLLEPLARAAPDHGLVFARVAGDRRLWALRVLAWLVVLGGLLVMLRDHLGADLLAL
                     LWVPLLALAGLSLWPGPPGAGSTALLGPALHRVGLLAAGAVLAVGPWLLALGLISGFD
                     QLIPGLLLVPSELPKALYFPYKAPPAEFWQVVALVVGAPLVGWLSLCLWPEVPRRAWL
                     LVGLFCAGWLLLPGRPDVLWQGGIFSWYYVPPALIWLGLLLQGRGWDGSAPPTRLGIV
                     ALYDRFLLAAGAFGLLQLYPLMDRVHGAWSYPFTLPLLALVLTRLPGWAARALPPGAV
                     PAARRVLFVALLALPLFWAWFIAGWRVNLWWDVPASLAAGRPVAAGWQPITVPHTSLI
                     AHTPDPAAYQAVYDLITAKTRPGEPIYAFPNEQAFYVICDRPRGSTRSYLLPGITSAA
                     EQQAAMDDLTRNGVRYVVMFVPSPGMEQGLGGYGPLDPLANY"
BASE COUNT          359 a          953 c         1133 g          570 t
ORIGIN      
        1 tcttgacatt ggccgagtag agcgcggcga tgctgttgcg atgggcgcta taggcgccgt
       61 gcagcttgag gaaggcgtcg gcgcgcagcg cgcggttatt ggcgcgcatg aagcgctggt
      121 agcgccccag cgtgagctgc gtttccgagc cttcctcgtc ggtgacggtg ccgaaggtca
      181 ggtcggcgtt gttcagcatg ctgaagacgg tgctgggcgc ggcggcgagt tcgcccacct
      241 gggccagcag ttgctcttcg gcttgcggca gcacgtgggg cttgccgcgt agcagatcct
      301 cgaagtcgtg ggcgtagaca cgcaaccccg gttcctcgtc catgaggcgg tccagggtgc
      361 cgtcgggcag ggccagcagc tccgggcgca cgaacgcagt agcggcgtgg gcgcggacgc
      421 ccagttgctg cacgcggtcg gccagcgact ggtaggttga gttggcggtg tcctcatcgc
      481 ggcgcaggaa ggcatagatc gccaggcggt cgagccggcg gcctagcgtg tcctgcaggg
      541 tcatggcggc cagcaaggtc ttgccgtcgg cggccaggcg gccctgatag ccggtgaact
      601 cgtccagcag ggcgggcaag gccgcgaagt cttcttccca agcggcattc gtcgggtaga
      661 tggtgctcaa atcccacgta tattgctcgg ggatttcggc gcgggtgggc agggtcttgg
      721 tcatggattg tctatcctct ctctctccct agcgagccgg gtccggcgtg gaccggcggc
      781 gtgctaacat tcatgagctt ctattatact atgttagccg tgagcggtca gccgtcaggg
      841 atcaggtatg tgccaagcac cgctgtgtga atacatgcgg gcaatggacg ccgggcagag
      901 aatatagctc ccggcaatcg ctgcatctac atacggaacc gactcctgac cgctgacggc
      961 tgaccgctga tggctatttg cctttcgggc cacgctttgc tagaatatcg cgaaaagatg
     1021 aacgatccgc acgaacccgc cgctgtcttg acggtggctg cgacgaaccg ggcacgcgag
     1081 gccgcatccg ggtcgggcgc ggccccgctt gcgtctgcgc cggagcgcgg ggcgcgtcgc
     1141 cgcgatgccg ccgtggcggg gttgctggcc ctcgccggag cgctgctcta tatccccacc
     1201 gcgcccttct acttccgtcc cgacgacgac gggcacgcca tcttcgacgc cctgctggtg
     1261 cgccaggggc tgcggcccta cctggacttc ttcgtccacg actcgccctt catccactac
     1321 tggaacgcgc tgctcttgca ggtcggcggc gtggacatgc tgacgctgcg ctacgggttg
     1381 gtgccgttca agatcctgct gttgccggcg atctatctgc tggcgcgccc ggtggcgggg
     1441 cggctcgtgg cggcgctggc tgcggccatg gtgctgatgt cggacagcgc ggtcaacttc
     1501 ggcatgcccc accccggatg gtatgcgatg gcactggccc tgctcgggct gctgggcatg
     1561 cgggcctggt tgggtagcgg gcgcggggcc tggctggtgg gcgcggggtt ggcgtttggc
     1621 gtggcggtcg atgtgaagca gaacctcggc ctgttcctgc tgggcgcggg gggcctgttc
     1681 ctgctggaac cgctggcgcg ggcggcgccg gaccacggcc tggtcttcgc gcgcgtggcg
     1741 ggcgaccggc ggctctgggc gctgcgggtg ctggcctggc tagtggtgct aggcgggctg
     1801 ctggtgatgc tgcgtgacca cctgggcgcc gatctgctgg cgctgttgtg ggtgccgctg
     1861 ctggcgctgg ccgggctgag cctctggccg gggccgcccg gagcgggcag tacggcgctg
     1921 ctggggccgg cgttgcaccg ggtggggctg ctggcggcgg gcgcggtgct ggccgtcggg
     1981 ccctggttgc tggcgctggg gctgatttcg ggcttcgatc aactgatccc cggcctgctg
     2041 ctggtgccga gcgaattgcc gaaggcgctg tacttcccct acaaagcgcc ccccgccgag
     2101 ttctggcagg tggtggcact ggtcgtgggc gcgccgctgg tggggtggct gagcctgtgc
     2161 ctctggccgg aagtgccgcg ccgggcgtgg ctgctggtgg gtctgttttg cgccggctgg
     2221 ctgctgctgc cggggcgccc ggatgtgctg tggcagggcg gtatctttag ctggtactac
     2281 gtgccgcccg cgctgatctg gctgggcctg ctgctgcaag ggcgcggctg ggacggcagc
     2341 gccccgccca cgcggctggg catcgtcgcg ctctatgacc gcttcttgct ggcggcgggc
     2401 gcctttggcc tgctgcaact ctatccgctc atggaccggg tccacggcgc ctggagctat
     2461 ccgttcacgc tgccgctgct ggcgctggtc ctgacgcgcc tgccggggtg ggcggcgcgg
     2521 gcgctgccgc cgggcgcggt gcccgccgcg cggcgcgtgc tgttcgtcgc gctgttagcg
     2581 ctgccgctgt tctgggcctg gttcatcgcc gggtggcggg tgaacctgtg gtgggatgtg
     2641 ccggcctcgc tggcagcggg ccgcccggtg gccgcgggct ggcagccgat cacggtgccg
     2701 cataccagcc tgatcgcgca cacgcccgat cccgcagcgt accaggccgt ctacgacctg
     2761 atcacggcga aaacgcgccc cggcgagccg atctatgcct tccccaacga gcaggctttc
     2821 tatgtgatct gcgaccgccc gcgcggcagc acgcgtagct acctgttgcc gggcatcacc
     2881 agcgcggcgg aacagcaggc ggcgatggac gacctgaccc gcaacggcgt gcgctacgtc
     2941 gtgatgtttg tgccgagtcc gggcatggag caggggctgg gcggctacgg gccgctcgac
     3001 ccgctcgcca attat
//
LOCUS       DASUYC010000376         2966 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_15809,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000376 DASUYC010000000
VERSION     DASUYC010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15809"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2422)
                     /locus_tag="VF276_06200"
     CDS             complement(<1..2422)
                     /locus_tag="VF276_06200"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6606493.1"
                     /translation="MIDTKPSFGRWLKERRKALDLTQFALAERVGCAEVTIGRIEADE
                     RRPSRQVAELLAEALGIPPDDRAAFVQFARTGHTGEAAAPLMPTSATPPAAAPTPVPP
                     LAAPPVRAMAAPLTRLIGRDQTLAAVREALHRTGGRLLTLTGPPGVGKTRLAMQVAAD
                     MGGEFAGGVGFVPLAPISDPDYVPTAVAQALGLQDTGAAPLPDRVLTYLRDKALLLVL
                     DNFEHLLGAAPLLVEWLAASPRLTVLVTSRAALRVRGEQQFAVPPLALPDPAHLPPLS
                     ELARYPAVTLFVERAAGVQADFALTEENAAAVAGICARLDGLPLAIELAAARSKLLTP
                     AAMLARLAARLPLLAGGPRDLPARQQTLRAAIGWSYDLLDAGEQALFARMSVFVNGCT
                     LDAVEAVCNATGDLPFDALDGVSSLFDKSLLRQDEPSEAEPRFNILDTIREFALEALA
                     ARGEVDTIRRRHFAHYLAEAEQADPLLQGAEQTAWLERLEADHDNFRAALTWARDSGD
                     AEALARLAGALWRFWYVHSHFHEGRGWLNQALAHQDAVSPPVRVRVLNGTAWLAYNQG
                     DFARARDLAGQSLALARSLDNKWEIGQAMATLGSVELGQGNFAAATAHYEHGLAVMRA
                     LAHSWGISYMLNFLGLTAQLAGDSAQATALLEESLALSRHLHDIRGMAFTLLNLGRAA
                     HEQGDDARAAALLEESLELSAELGNKEYVATCLSYLALLARLRTEYRHAVLLYRESLH
                     LLLELGHRESLAVGLEGLAGAVQGEGHPEHAVRLLAAAAALREAIGAPLPPSQRPYYE
                     QTLAEVRAQ"
BASE COUNT          412 a         1030 c         1114 g          410 t
ORIGIN      
        1 actgagcgcg cacttcggcc agcgtctgct cgtagtaggg gcgctgcgag ggcggcaggg
       61 gcgcgccgat ggcttcgcgc agggcggcgg cggcggccag caggcgcacc gcgtgttccg
      121 ggtggccctc gccctgcacc gccccggcca ggccctccag gcccacggcg aggctctcgc
      181 ggtggcccag ttccagcagc aggtgcaggc tctcgcggta gagcagcacg gcgtggcggt
      241 actcggtgcg cagccgggcc agcagcgcca ggtagctcag gcaggtggcc acatactcct
      301 tgttgcccag ttccgccgac agttccaggc tttcttccag cagcgcagcg gcgcgggcgt
      361 cgtcgccttg ctcgtgggcg gcgcggccca ggttgagcag ggtgaaggcc atgccgcgga
      421 tgtcgtgcag gtggcgcgag agggccaggc tttcttcgag cagcgccgtg gcctgggcgc
      481 tgtcgcccgc caactgcgcc gtcaggccca ggaagttgag catgtaggag atgccccacg
      541 agtgggccag cgcgcgcatg acggccagcc cgtgctcgta gtgcgcggtg gcggcggcga
      601 aattgccctg gcccagttcc acgctgccca gcgtcgccat ggcctgcccg atctcccact
      661 tgttgtcgag gctgcgggcc agggccagac tttgcccggc caggtcgcgg gcgcgggcaa
      721 aatcgccctg gttgtaggcc agccaggcgg tgccgttgag cacacgcacg cgcaccggcg
      781 gggacacggc gtcctggtgg gccagcgcct ggttcagcca gccgcggccc tcgtggaagt
      841 ggctatgcac ataccagaag cgccacagcg cgccggccag ccgggccagc gcctcggcgt
      901 cgccgctgtc acgggcccag gtcagggcgg cgcggaagtt gtcgtggtcc gcctccaggc
      961 gctccagcca cgcggtttgc tcggcgccct gcagcagcgg gtcggcctgc tcagcctcag
     1021 ccaggtagtg ggcgaagtgg cggcggcgga tcgtgtccac ctcgccgcgc gccgccaggg
     1081 cttcgagggc gaattcgcgg atcgtgtcga ggatgttgaa gcgcggctcg gcctccgacg
     1141 gctcgtcctg gcgcaacagg ctcttgtcga acagcgagga cacgccgtcg agcgcgtcga
     1201 agggcagatc gccggtggcg ttgcagaccg cttccacggc gtcgagcgtg catccgttga
     1261 caaagacgct catgcgggcg aacagggcct gctccccggc gtcgagtagg tcgtagctcc
     1321 agccgatggc ggcgcgcagg gtctgctggc gggcgggcag gtcgcgcggc cccccggcca
     1381 gcagcggcag gcgggcggcc agccgggcca gcatggccgc gggcgtgagc agcttgctgc
     1441 gggcggcggc cagttcgatc gccagcggca ggccgtcgag ccgggcgcag atgccggcca
     1501 cggcggcggc gttctcctcg gtcagcgcga aatcggcctg caccccggcg gcgcgctcca
     1561 cgaacagggt cacggcgggg tagcgggcca gctcgctcag cggcggcagg tgggccgggt
     1621 cgggcagggc cagcggcggc acggcaaact gctgctcgcc gcgcacgcgc aacgccgccc
     1681 ggctcgtgac tagcacggtc agccgcgggc tggcggccag ccactcgacc agcagcgggg
     1741 cggcccccag cagatgctcg aagttgtcga gcaccagcag cagcgccttg tcgcgcaggt
     1801 aggtgagcac gcggtcgggc agcggcgcgg cgcccgtgtc ctgcaagccc agtgcctggg
     1861 ccacggcggt cggcacataa tcggggtcgc tgatgggggc gaggggcacg aagccgacgc
     1921 ccccggcgaa ctcgccgccc atgtcggcgg ccacctgcat agcgagccgc gtcttgccga
     1981 cgccgggcgg cccggtcagg gtcagcaggc gaccccccgt gcggtgcaac gcctcgcgca
     2041 cggcggccag cgtctggtcg cgcccgatga ggcgggtgag cggggcggcc atggcgcgca
     2101 ccggcggcgc ggcgagcggc ggcacagggg tgggcgcggc ggcgggcggg gtcgcgctgg
     2161 taggcatcag cggcgcggcg gcctcgccgg tgtggccggt gcgcgcgaat tgcacgaagg
     2221 ccgcgcggtc gtccggcggg atgcccagcg cctcggccag caactcggcc acctggcgcg
     2281 agggccggcg ctcgtcggcc tcgatgcgcc cgatggtcac ttccgcgcag cccacgcgct
     2341 cggccagggc gaactgcgtc aggtcgaggg ccttgcggcg ctctttgagc cagcggccaa
     2401 aggacggttt cgtatcgatc attccaccca attccggtat cctagtgggg cgcgtgcgcg
     2461 accagcccgc cgcccccgac cggcggcacc gctggattct gcgtattata gccgatctcg
     2521 cgcgcaaacg gaagcaggcg gcctccgtct gctctgcaca aattcaccgc gaaggaacga
     2581 aggcaacgaa gattttatcg atccgtgaga ttcttcgcgc tcttcgttcc ttcgcggtgg
     2641 acacacatca tccgtcgtgt ctatagaata tggcgcgcgc cgcccggcag gggctacagc
     2701 tttggaccga ttttgcgcct gggtaaatgc ccccgtgcgc ccggacacgg caaagcaccc
     2761 cgcccctggc gaggcgaggt gctgcggtgt acgcgcgagg gtgggcgggc gctcagccgc
     2821 cccagaccac gccgaacagg tgcgggctgc cggcgacggc cagcagcagc gcgatgccca
     2881 ggtagcgata cagtgccgtt ttcattgttg cttcctccac agggcgcggc gggcgctcag
     2941 ccctccccgc cccagacgat accgca
//
LOCUS       DASUYC010000377         2427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1400278, whole genome shotgun sequence.
ACCESSION   DASUYC010000377 DASUYC010000000
VERSION     DASUYC010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1400278"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..431
                     /locus_tag="VF276_06205"
     CDS             75..431
                     /locus_tag="VF276_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HEX6606494.1"
                     /translation="MATTLYGITNCDTVKKARAWLAGAGVDHHFHDFRKDGVPPEHLD
                     RWIQAAGWEKLLNRQGTTWRKLDTGAQSQAGNAAGARALMLAQPSVIKRPVVEWPDGS
                     VTVGFDPGAWPVRKSS"
     gene            530..1276
                     /locus_tag="VF276_06210"
     CDS             530..1276
                     /locus_tag="VF276_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEX6606495.1"
                     /translation="MKQSVLFAALGLAALGVHAQEVGRVLSSNPVIEQVAVPRQVCSQ
                     MPVVQPQTSGGGGLLGAIAGGVIGSTIGGGAGTGAAIVAGTALGAVVGNNIEANNQRY
                     AQSVPQCVTETTSENRTTGYDVTYEYAGREYSTRMPYDPGATIMLNVSPVASANTRPA
                     NAPVVVAPPVQAVQSTQPVQQVQVQPVQTVQAAPVVVTTPAPVVYAPYAAYPAYPAYP
                     YPYPAYRPWYPNISLGFVFGGGHRHGRWRH"
     gene            1384..1701
                     /locus_tag="VF276_06215"
     CDS             1384..1701
                     /locus_tag="VF276_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HEX6606496.1"
                     /translation="MTTATLAAASVQTQANVYFDGKCVSHGVTLADGTKKSVGVILPA
                     TLTFNTGAPETMECVAGACEYKLAGSDEWRQSGPGGKFSVPANSSFEIRVAEAYHYIC
                     HFG"
     gene            1710..>2427
                     /gene="argG"
                     /locus_tag="VF276_06220"
     CDS             1710..>2427
                     /gene="argG"
                     /locus_tag="VF276_06220"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX6606497.1"
                     /translation="MSTILENLPLGQKVGIAFSGGLDTSAALHWMRKKGAIPYAYTAN
                     LGQPDEPDYDAIPRKAMEYGAEKAVLVDCRKQLAHEGIAALQCGAFHVATAGQMYFNT
                     TPLGRAVTGTMLVAAMKEDDVHIWGDGSTFKGNDIERFYRYGLLTNPALRIYKPWLDQ
                     AFINELGGRKEMSEFLIKSGFDYKMSVEKAYSTDSNMLGATHEAKDLEFLDNGIAIVQ
                     PIMGVPFWREDIDVAYEEVRI"
BASE COUNT          422 a          862 c          793 g          350 t
ORIGIN      
        1 cggcgccgac ttctatcgcg ccgtcgccgc gctggggctg gcgttcttcg aacgcgaggc
       61 ctgacaatgg cgggatggcg accaccctct acggcatcac gaactgcgac acggtgaaga
      121 aggcccgtgc ctggctggcc ggcgccggcg tggaccacca cttccacgac ttccgcaagg
      181 acggcgtgcc gcccgagcac ctggaccgct ggatccaggc ggcggggtgg gaaaaattgc
      241 tcaatcggca gggtacgacg tggcgcaagc tcgacaccgg cgcccagtcg caagcaggca
      301 acgcagccgg cgcccgcgcc ctgatgctgg cgcagcccag cgtgatcaag cgcccggtgg
      361 tggagtggcc ggacggcagc gtgaccgtcg gcttcgaccc gggcgcctgg ccggtgcgca
      421 agagttcctg agccgggact ttttaccggg cctttaccgt aaagctctgc agaacctgtt
      481 gccgctgcat aaactttcag caccaagcag ttcaacgagt ttttccgcca tgaaacagtc
      541 cgtccttttc gccgcgctgg gcctcgccgc ccttggcgtg cacgcgcagg aagtcggccg
      601 tgtcctttcc agcaaccccg tgatcgagca ggtggccgtg ccccgccagg tgtgcagcca
      661 gatgccggtc gtccagcccc agaccagcgg cggcggcggc ctgctgggcg ccatcgcggg
      721 cggcgtgatc ggcagcacca tcggcggcgg tgcgggcacc ggcgcggcca tcgtggccgg
      781 cacggccctg ggcgccgtcg tcggcaacaa catcgaggcg aacaaccagc gctacgcgca
      841 atcggtgccg cagtgcgtga ccgagaccac ctccgagaac cgcacgacgg gctacgacgt
      901 gacctacgag tacgccggcc gcgagtactc cacccgcatg ccgtacgacc ccggtgcgac
      961 gatcatgctg aacgtctcgc cggtcgcgag cgcgaacacc cggccggcca acgcgccggt
     1021 ggtggtcgcc ccgcccgtgc aggcggtgca atcgacgcag ccggtgcagc aggtgcaggt
     1081 gcagcccgtg cagacggtgc aggccgcccc ggtggtcgtc acgaccccgg cacccgtggt
     1141 ctatgcgccc tacgcggcgt atccggcgta ccccgcctat ccctatccgt accccgcgta
     1201 ccggccctgg tacccgaaca tctcgctggg cttcgtgttc ggcggcggcc atcgccacgg
     1261 ccgctggcgc cactgagcgg ccatccggcc ggaacgcaag gggcgcccgc gggcgccctt
     1321 tttcatgccc ggccgggcgg cgcgcgctcg cctaaaattg cgggtttcgc cggaagccaa
     1381 cccatgacca ccgcaaccct cgccgccgcg tccgtgcaga cgcaggccaa cgtgtacttc
     1441 gacggcaagt gcgtcagcca cggcgtcacg ctggccgacg gcacgaagaa gtcggtgggc
     1501 gtgatcctgc cggccaccct gacgttcaac accggggcgc cggagaccat ggaatgcgtg
     1561 gccggtgcct gcgagtacaa gctcgccggc agcgacgaat ggcgccagtc cggcccgggc
     1621 gggaagttct cggtgcccgc caactccagc ttcgagatcc gcgtggccga ggcctaccac
     1681 tacatctgcc acttcggctg agcacagaca tgagcaccat cctcgaaaac ctgcccctgg
     1741 ggcagaaggt cggcatcgcc ttttccggcg gcctggacac cagcgccgcg ctgcactgga
     1801 tgcgcaagaa gggcgccatc ccctacgcct acaccgccaa cctgggccag cccgacgagc
     1861 cggactacga cgcgatcccg cgcaaggcca tggagtacgg cgccgagaag gcggtgctcg
     1921 tggactgccg caagcagctg gcccacgaag gcatcgccgc cctgcagtgc ggcgccttcc
     1981 acgtcgccac cgcggggcag atgtacttca acaccacgcc gctgggccgg gccgtgacgg
     2041 gcacgatgct ggtggcggcg atgaaggagg acgacgtcca catctggggc gacggcagca
     2101 ccttcaaggg caacgacatc gagcgcttct accggtacgg cctcctcacc aacccggcgc
     2161 tgcgcatcta caagccctgg ctggaccagg ccttcatcaa tgaactcggc ggccgcaagg
     2221 aaatgtccga attcctgatc aagtccggtt tcgactacaa gatgtcggtg gagaaagcct
     2281 attcgaccga ctccaacatg ctgggcgcca cgcacgaggc caaggatctg gagttcctcg
     2341 acaatgggat cgccatcgtc cagccgatca tgggcgtgcc gttctggcgc gaggacatcg
     2401 acgtcgccta cgaagaggtc cggatca
//
LOCUS       DASUYC010000378         2642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_924987,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000378 DASUYC010000000
VERSION     DASUYC010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924987"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="VF276_06225"
     CDS             <1..773
                     /locus_tag="VF276_06225"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEX6606498.1"
                     /translation="AFTDPYSDQGLPVPLRQARADLRPLVRGMVDVGGKVIYTHRNLG
                     AGQRRFVTLHELGHYVLPWHNSLLQYCSESDLSPAARQTWEREANLFAGACLFQGSRF
                     TEEAARTSFGWPAVRRLAARYEASLEAAARQYTLSRSGPCMLLVAGLRPDIADRQEAT
                     SGEPLLLVRYATVSPAWRRRYGAAGVPAPGTALPWAHPATSVMLSPSRAVLAMPVDGP
                     LGGDPAWPLRAELLSNGYEVLMLAMPGASPAYFSKGAG"
     gene            complement(749..1147)
                     /gene="tatB"
                     /locus_tag="VF276_06230"
     CDS             complement(749..1147)
                     /gene="tatB"
                     /locus_tag="VF276_06230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HEX6606499.1"
                     /translation="MGFEVFGVGVPEMVIILVVALIFLGPEKLPEAARTVGGWVREIR
                     GISSEAMSVWQETLQVGDTIKSSVSIDGAGRSAPRPATPPQLPVAYTPPDAAEAPAEP
                     ATLDYPAPFAPPKTAPAPAEPLAYPAPFEK"
     gene            complement(1181..1513)
                     /gene="cutA"
                     /locus_tag="VF276_06235"
     CDS             complement(1181..1513)
                     /gene="cutA"
                     /locus_tag="VF276_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002731134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HEX6606500.1"
                     /translation="MDTEPSNDNLVLLYVPCGSEDEALGLGRALVEARLIACANLHPI
                     RSVYAWEGRVVDEAEVVLLAKTTEAQAAAAEAEIRRRHSYEVPCILRLPTAGVNADYL
                     AWIRAAVS"
     gene            complement(1669..>2642)
                     /locus_tag="VF276_06240"
     CDS             complement(1669..>2642)
                     /locus_tag="VF276_06240"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX6606501.1"
                     /translation="LAVTVYYVINIILFPVTLLGYVIWAGKTALTGRRSGVSITAQAP
                     LMGRWTMHQFGTREDKPSAQLLMLLPSISPLAVRLISAAGMLAHRLTGYVPRAFRYPF
                     EGDIPRQYEAAARMAFFDAAVDRYLPNMTQLVILGAGFDTRALRLPKDLRVRSFEVDT
                     PRTQAIKRRMLAKTAIDATRVTFVPANFEKEDWFARLVGAGFDPSQPALFLWEGVVMY
                     LDREAVEDTLRKIGSCAAGSMVAFDYFTSEALTSQELYWRFARRSTRAVGEPLKFGVE
                     SRPPTSERLAELLQPCGLSLVEQHILGKETGQERAWGGFALAIVNRR"
BASE COUNT          410 a          908 c          893 g          431 t
ORIGIN      
        1 ttgccttcac cgacccctat agcgatcagg gcctgcccgt gccgttgcgt caggcgcgcg
       61 ccgacctgcg cccgctggtg cgcggcatgg tcgatgtcgg cggcaaggtg atctataccc
      121 accgcaacct gggcgccggg cagcgccgct tcgtcaccct gcacgaactg gggcattacg
      181 tgttgccctg gcacaacagc ctgctgcaat attgctcgga gtccgacctg agtccggcgg
      241 cgcgacagac ctgggagcgc gaagcgaacc tgttcgccgg ggcctgcctg ttccagggca
      301 gccgcttcac cgaggaggcg gcgcggactt ccttcggctg gccggcggtg cgccggttgg
      361 ccgcgcgcta cgaggcgtcg ctggaggccg ccgcgcgcca gtacacgctc agccggtcgg
      421 ggccgtgtat gctgctggtg gccgggctgc gccccgacat cgccgaccgg caggaggcca
      481 ccagcggcga accgctcttg ctggtgcgct acgccacggt ctcgcccgcg tggcggcggc
      541 gctacggcgc ggcaggggtg cccgcgcccg gcacggcgct gccctgggcg cacccggcga
      601 ccagcgtcat gctgtcgcct agccgggccg tgctggcgat gccggtggat gggccgctgg
      661 gcggcgaccc ggcctggccg ctgcgcgccg agctattgtc gaacggttat gaggtgttga
      721 tgctggcgat gccgggcgct agcccggcct atttctcgaa gggggccggg taggcgaggg
      781 gttccgcggg ggcgggcgcc gttttgggcg gcgcgaacgg cgccgggtag tcgagcgtgg
      841 ccggttcggc gggggcctcg gcggcgtcgg gcggggtgta ggccaccggc agttggggcg
      901 gcgtggcggg gcgcggggcc gaccgaccgg cgccgtcgat actcacgctg ctcttgatcg
      961 tgtcgccgac ctgcagcgtc tcctgccaca cgctcatggc ctcgctgctg atgccgcgga
     1021 tctcgcgcac ccagccgccg acggtgcgcg cggcttcggg cagcttctcc ggccccagga
     1081 aaatcagcgc cactaccagg atgatcacca tctcgggcac gccgacgccg aacacttcaa
     1141 atcccatggc aaatctcgcg ggcgccgggc gccctctccc ctagctgacg gccgcgcgaa
     1201 tccaggccag gtaatcggcg ttgacgcccg ccgtgggcag ccgcaaaatg cagggcacct
     1261 cgtagctgtg gcggcggcgg atctcggcct cggcggcggc ggcctgggcc tccgtggtct
     1321 tcgccagcag taccacctcg gcttcgtcca ccacgcggcc ttcccaggca tatacggagc
     1381 ggatcgggtg caggttggcg caggcgatca gccgcgcctc gaccagcgcc cgcccgaggc
     1441 ccaacgcctc gtcctcgctg ccgcagggca catagagtaa caccaggtta tcgttgctcg
     1501 gttccgtgtc catgcatcta ttatagccga aggatcgcgc cattgcctag ccgtagccac
     1561 ggcagagggc gcggcgggaa gcatcactgt catagcgcaa gcgcgccgcg ccccacagaa
     1621 atccggcctg ctgcaccaaa ctcaccaccg catcccgcaa gcggcgcatc accgccggtt
     1681 cacgattgcc agagcgaagc ccccccaggc gcgctcctgg cccgtctctt tcccgagaat
     1741 gtgttgctca acaagtgaga gtccgcaagg ctggaggagt tcggccaggc gctcactggt
     1801 cggtggccgg ctttcgaccc cgaatttcag tggttcgccg acagctctgg tgctccttct
     1861 cgcgaaacgc caatacagct cctgcgaggt gagtgcctca ctggtgaagt agtcgaacgc
     1921 aaccatactg cccgcggcac agcttccgat cttccgcagc gtgtcctcga ccgcttcccg
     1981 gtccagatac atcaccacac cctcccacag gaagagcgcc ggttggctgg gatcgaaccc
     2041 ggcacccacg agtcgcgcga accagtcttc cttctcgaag ttggccggga caaacgtgac
     2101 cctggtcgcg tcgatggcgg tcttcgcgag catccggcgc ttgatcgcct gggtcctggg
     2161 ggtatcgacc tcgaaggatc ggacccgcag gtccttgggc agccgcaacg cgcgggtgtc
     2221 gaagcctgcg ccaaggatga caagctgtgt catgttgggc aggtagcgat ctaccgccgc
     2281 gtcgaaaaac gccatgcgcg cggcggcctc gtactgtcgc ggaatgtccc cctcgaaagg
     2341 ataccggaat gccctgggca cgtagcctgt cagccggtgc gcaagcatcc ccgcggcgga
     2401 tatcaaacgc acggccaggg gcgagatgct cggcagtagc atcagcagtt gcgccgatgg
     2461 tttatcttcc ctggtcccga actgatgcat cgtccagcgg cccatcagcg gcgcttgcgc
     2521 ggttatcgag acccccgacc ggcgtccagt gagggccgtc ttgccggccc agatcacata
     2581 gccgagcaac gtgaccggga acaggatgat gttgataacg tagtagaccg tgaccgccag
     2641 ga
//
LOCUS       DASUYC010000379         2303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_267788,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000379 DASUYC010000000
VERSION     DASUYC010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267788"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..1139)
                     /locus_tag="VF276_06245"
     CDS             complement(27..1139)
                     /locus_tag="VF276_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HEX6606502.1"
                     /translation="MPWSQVTVVDQREEFVRLARQPDANVSELCRRFAISRKTGYKWL
                     ARESYEDRSRRPCTSPNRTDQSLEARVLAIRDRHPAWGGRKIAHVLQRDADVQLAPST
                     VNSVLRRNGRISEEASQAGMRWQRFEHDAPNSMWQMDFKGHFATQTQRCHPLTVLDDH
                     SRFNIVLQALSDEQLVSVQPVLQRAFERFGLPQRINTDNGPPWGSGGQQAITALGVWL
                     VRLGVRLSYSRPLHPQTNGKDERFHRTMKAEVLCRQQFRDIQHAQNHLARWRHIYNYE
                     RPHEALDMLTPSSRYRPSTRSMPKELPPIEYEPGDLVRQVQKGGWLTLHGRPIHVSKA
                     LAGQPVACRPLADQDGEFLIFFCHQQVGQINLRNAC"
     gene            1390..1596
                     /locus_tag="VF276_06250"
     CDS             1390..1596
                     /locus_tag="VF276_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEX6606503.1"
                     /translation="MKTKVLSAAVLAVSMLGLGGCATMDRTTVGTVGGAVVGGLVGSA
                     IGGTGATILGAGAGAYLGNQAAKR"
     gene            1823..2032
                     /locus_tag="VF276_06255"
     CDS             1823..2032
                     /locus_tag="VF276_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEX6606504.1"
                     /translation="MKTKFLSAAVLAVSMLGLGGCATMDRTTVGTVGGAVVGGLVGDA
                     IGGTAATVLGAGAGAYLGNQAAKRY"
     gene            complement(2055..>2303)
                     /locus_tag="VF276_06260"
     CDS             complement(2055..>2303)
                     /locus_tag="VF276_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HEX6606505.1"
                     /translation="YHAPYYTRHVVSPAQVLEVSGGRIRAQANYAVFRTRPGDASEVY
                     NVGRYIDAIERTPAGLKLASRTCVYDSEMVLNSLIYPV"
BASE COUNT          350 a          766 c          770 g          417 t
ORIGIN      
        1 acatgggtga cagtccgagc ggcagcttag caggcgttcc tcaggttgat ctgccccact
       61 tgctggtggc agaagaagat gaggaattcg ccgtcctgat cagccagtgg tctgcaggcc
      121 actggctggc cggccaaggc cttgctgacg tggatcggcc ggccatgcag cgtcagccag
      181 ccgccttttt ggacctgccg caccagatct cccggctcgt attcgatcgg cggcagctcc
      241 ttgggcatcg agcgggtgct gggccgatac cggcttgacg gggtgagcat gtccagcgcc
      301 tcgtggggcc gctcatagtt gtagatgtgt cgccagcgag ccaggtggtt ttgagcatgc
      361 tggatgtctc ggaactgctg gcggcaaagc acctcggcct tcatcgtgcg atggaagcgt
      421 tcgtcctttc cgttggtctg cgggtgtagc ggccggctgt agctcagtcg cacacccaag
      481 cgaaccagcc acacccccag ggcggtgatc gcctgttgac cgccgctgcc ccaaggcggg
      541 ccgttgtccg tattgatgcg ttgcggcagg ccaaaccgct cgaatgctcg ctgtagcacc
      601 ggctgcaccg acaccagctg ctcgtcactc agcgcctgca gcacgatgtt gaagcgcgag
      661 tgatcgtcca gcactgtcaa cgggtggcag cgctgcgtct gagtggcgaa gtggcccttg
      721 aagtccatct gccacatcga gttcggcgcg tcatgctcga agcgctgcca acgcatgccg
      781 gcctggctgg cttcttcgct gatgcggccg ttgcgccgca gcacggagtt cactgtgctg
      841 ggcgcgagct gaacatccgc gtcacgctgc agcacatggg cgatctttcg cccgccccag
      901 gccgggtgtc gatcgcgtat ggccaggacc cgcgcctcca gggattggtc agtgcgattc
      961 ggactggtgc aaggccggcg tgaccggtcc tcgtagctct cgcgagccaa ccacttgtac
     1021 ccggtcttgc ggctgatggc gaagcggcgg caaagctcgc tgacattggc gtccggctgg
     1081 cgcgccagcc ggacaaactc ttctctctga tcgaccacgg tgacttggct ccagggcaac
     1141 gtcgactccc gctaactcag cgggaaaagt gttacccatg tcctcgcaca cctgtcacct
     1201 atgtccccgg tctatacacc ccgaagggga gagggagaaa gtcgaagcct tcacccttcc
     1261 ctgccggcag acgacacctc ccggcgcacg gttcctacag tcccgcagag cctgcgccgc
     1321 ctacattcct tcgacaggtt gccgcgcctc ccgcggcttt tcgaacgaat cgaactggaa
     1381 ggtgaatcca tgaagaccaa agtcctttcc gcggctgtgc tggccgtttc catgctgggc
     1441 ctgggtggct gtgccacgat ggaccgcacg accgtcggca cggtcggcgg cgccgtggtc
     1501 ggtggcctcg tgggcagcgc catcggtggc accggtgcga ccatcctggg ggccggcgcc
     1561 ggcgcctacc tgggcaacca ggccgccaag cgctgattcc gcggcacgcg gcatccatgg
     1621 gccagggcct cgcgtcctgg ccctttttca tgcccgccgc ggtgcctcag cgcccgaccc
     1681 gcggcctgca cccttcccgg ggggcagacg acaggggcag tcaagcggtt cctacagtcc
     1741 cgcagagccc cgctgcctac attggttggc acaggtggcc gcgtcgcggc cggaacgttg
     1801 acccgattcg gaaggtgatc ccatgaagac gaaattcctc tccgcggccg tgctggccgt
     1861 ttccatgctt ggtctgggcg gctgcgccac gatggaccgc accaccgtcg gtacggtggg
     1921 cggcgcggtg gtcggcgggc tggtgggcga tgcgatcggc ggcaccgccg ccacggtgct
     1981 gggcgccggc gccggtgcct acctgggcaa ccaggcggcc aagcgctact gagcgggggc
     2041 ggcgcgcgcc gccttcacac cggatagatc agcgagttga gcaccatctc gctgtcgtag
     2101 acgcacgtgc ggctggccag cttcaggccg gccggcgtgc gttcgatcgc gtcgatgtag
     2161 cggcccacgt tgtagacctc gctcgcatcg cccggccggg tgcggaacac cgcgtagttg
     2221 gcctgcgcgc ggatgcgccc gccggacacc tcgagcacct gcgcggggct gaccacgtgg
     2281 cgcgtgtagt agggcgcgtg gta
//
LOCUS       DASUYC010000380         2227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1155393, whole genome shotgun sequence.
ACCESSION   DASUYC010000380 DASUYC010000000
VERSION     DASUYC010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155393"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..410
                     /locus_tag="VF276_06265"
     CDS             240..410
                     /locus_tag="VF276_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606506.1"
                     /translation="MDESAGSARSILSRGSYLSILSLFSFASILSIGSAGSILSIGGA
                     GGVLRVGRRAAR"
     gene            556..762
                     /locus_tag="VF276_06270"
     CDS             556..762
                     /locus_tag="VF276_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606507.1"
                     /translation="MAQDGKLPNPVARRAARAGSVALGVFLGFVLLDSNPLGAIWQAL
                     VIGAIVFIALFLLETYFENRRDSV"
     gene            complement(773..>2227)
                     /locus_tag="VF276_06275"
     CDS             complement(773..>2227)
                     /locus_tag="VF276_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX6606508.1"
                     /translation="MLSWLFRCPLPDGDAARKWRILNVMLLVFMALVIGGTAATATLQ
                     TSPLSLPTVATIGMLLVLYAVNRRGWLVPAAVGFVIGLEGIVVLYSLANPDGDPVANA
                     LIPTLGALPVALSATLLSWRAVPFTVLGVSGVTVWLYAQGMATVVAYLAAHNMGAQFL
                     PTVIILFIAVGALTALPNRQVEQTLARLEHRNGELEQANRDLAAQRERDLRLQANLGT
                     LATELSAVSSRQGVAVTTQASSISQVVSAVAELDSTAGQIADRALEVRQAADQALTSV
                     QQGQSLLLRSREAVERNRAEVQLVIQRMRDLDQLTRQVTRFVDQIRHLAEDTHLLALN
                     ATIEAAGAGGVGRRFQVVADEVRALSNQSSAIVNQIQQVVSQLQQAGQVALHATQTSA
                     EVAEAAAQLADDVRITQDQIVTAVQQTNALVHLISTASTQQTSATQEMTYTMEGIAAI
                     ARATSEDTRALDRAVGEMLQAATLLSAAATARAG"
BASE COUNT          339 a          733 c          779 g          376 t
ORIGIN      
        1 atggtaccct cctcattgcg gcgccgccct accgggcggt gcggcctcca atgaatgatg
       61 aagacgatca ccggcgataa ttgaccggta caaagcccat tctagcgtct gtcgattaag
      121 gattcctgta caatgcgcgc aggcaagtta caaggccgta aggatcacgc cggcgcgtgg
      181 atagcggaag gcggatgcgt gaatcgcccg caagcgcagc cttggaggag caaccacaga
      241 tggacgaatc ggccggttcg gcgcgtagca tcctgagccg cggcagctac ctcagtatct
      301 tgagcctgtt cagcttcgcc agcatcctga gcatcggcag cgcgggcagc atcctgagca
      361 tcggcggcgc gggcggcgta ctgcgcgttg ggcgccgggc ggcccgatag cggcctgcgc
      421 tgccccgtac ggcggccccc cacttgctga agacgctgtg cagggcgatc gagcgggccg
      481 gcgcggtccg gcccggcggc gcggcaccat tcgcgccgcg ccctgcgcgt cattccgagt
      541 tgaagggggt taccaatggc acaggacggc aagctgccga atcccgtggc ccggcgggcg
      601 gcgcgggcgg gctctgtggc cctgggggtg tttctcgggt ttgtgttgct ggactcgaat
      661 ccgctcggcg cgatctggca ggcgttggtg atcggcgcga tcgtcttcat cgccctgttt
      721 ctgctggaaa cgtattttga aaaccgccgc gactcggtgt gatcgccggt agttagccgg
      781 cccgcgccgt cgccgccgcg ctcagcaggg tcgccgcctg cagcatctcg ccgacggcgc
      841 ggtcgagggc gcgggtgtct tcgctggtgg cccgcgcgat ggccgcgatg ccctccatcg
      901 tgtacgtcat ttcctgggtg gccgaggttt gctgcgtgct ggccgtcgaa atcaggtgga
      961 ccagggcgtt ggtctgttgc acggcggtca cgatctggtc ctgggtgatg cgcacgtcgt
     1021 cggccagttg cgcggccgcc tcggcgacct cggcgctggt ctgcgtggcg tgcagcgcca
     1081 cttgcccggc ctgttgcaac tggctcacca cctgctggat ctgattcacg atcgcgctcg
     1141 actggttgga cagggcgcgc acctcgtcgg ccacgacctg gaagcgccgc cccacgccgc
     1201 ccgcccccgc ggcctcaatc gtcgcgttga gcgccagcag gtgtgtgtcc tcggccaggt
     1261 ggcggatctg gtcgacaaaa cgggtcacct gccgggtcag ttgatcgagg tcgcgcatgc
     1321 gctggatcac cagttggacc tcggcgcggt tgcgctccac cgcctcgcgg ctgcgcagca
     1381 gcaggctctg gccttgctgg acgctggtga gcgcctggtc ggcggcctgg cgcacctcca
     1441 gggcgcggtc ggcgatctgg cccgccgtgc tgtcgagttc ggccacggcg ctgaccacct
     1501 ggctgatcga actggcctgc gtggtgacgg cgacgccctg ccggctgctg acggcggaga
     1561 gctcggtcgc cagggtgccc aggttggcct gcaagcgcag atcgcgctcg cgctgcgcgg
     1621 ccaggtcacg gttggcctgt tccaactccc cgttgcggtg ctctaaccgg gccagcgtct
     1681 gttccacctg ccggttgggc agcgcggtca gcgccccgac ggcgataaac aggatgatga
     1741 ccgtcggcag gaactgggcc cccatattgt gggccgccag gtaggcgacc accgtagcca
     1801 tgccctgtgc gtacagccag acggtcacac cgctgacgcc cagcaccgtg aacggcaccg
     1861 cgcgccacga cagcagcgta gcgctgagcg cgacaggcaa ggcgccgagc gtggggatga
     1921 gggcattcgc caccgggtcg ccgtccggat tcgccaggct gtacaacacc acaatgcctt
     1981 ccaacccgat tacgaacccc actgccgccg ggacgagcca gccgcgccga ttgacggcat
     2041 agaggacaag cagcatgccg atggtcgcga cggtggggag actcaggggc gaggtttgca
     2101 gcgtcgcggt ggcggcggtt cccccgatga ccagggccat gaagaccaac aacatgacat
     2161 ttaagatccg ccacttgcgc gctgcgtcgc cgtcgggcag ggggcagcgg aacaaccagg
     2221 agagcat
//
LOCUS       DASUYC010000381         4810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_374523,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000381 DASUYC010000000
VERSION     DASUYC010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374523"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..2172)
                     /locus_tag="VF276_06280"
     CDS             complement(118..2172)
                     /locus_tag="VF276_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX6606509.1"
                     /translation="MANRLSQETSPYLLQHAHNPVDWYPWGPEALARARTEDKPILLS
                     IGYSACHWCHVMEHESFEDPATAALMNQHFVSIKVDREERPDLDSIYMAAVQTLTGQG
                     GWPMTVFLTPAGKPFFAGTYFPPDDRHGLPGFKRVLQSVARAYEQQRGEIDQSADDLA
                     AHLAEQSAIPAAPEALRPEFLDRAAAALAGEFDSRHGGLGGAPKFPQPMAWEFLVRQY
                     HRTRDARLLAMIEQTLRAMANGGIYDQVGGGFHRYSVDNHWLVPHFEKMLYDNALLSR
                     LYLHVYQITGEPFYRRIVEEVLDYVVREMTSPEGAFYSTQDADSEGEEGKFYVWTPDE
                     IDAVLTPEEARVARAYWGVTEQGNFEGKTILNVPRPADAVAQDLNLPLDTVPALLAAA
                     RGKLYAARAQRVWPGRDDKVLVSWNGLMLRSFAEAARVLGREDYRQVAQHNAAFVLGT
                     MLDGDRLWRTYKDGRAHLAGFLEDYAFYAAGLLALYEADFDPHWFLAARSLAATLLAQ
                     FADTERGGFFDTPADGEQLINRPKDLFESAIPSGNSAAAEVLLRLHYYTMEEDYYDAA
                     RGALEAAGALLAQYPSGVGRMLCVLDLYLAEVQEIALIGRPEAADTAALLAVVFQGYR
                     PHQVVAAGAADDSAAIAAVPLLEGRPQRDGKATAYVCVNYACQSPVTDPAALAAQLGG
                     SG"
     gene            2419..2595
                     /locus_tag="VF276_06285"
     CDS             2419..2595
                     /locus_tag="VF276_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606510.1"
                     /translation="MKKDSRLRAHAARAQGLVEYGLILVLVMVVVVVVVALAGDDLNT
                     MFSVVASEVLAITP"
     gene            2728..2804
                     /locus_tag="VF276_06290"
     tRNA            2728..2804
                     /locus_tag="VF276_06290"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2762..2764,aa:Arg,seq:gcg)
     gene            2845..2931
                     /locus_tag="VF276_06295"
     tRNA            2845..2931
                     /locus_tag="VF276_06295"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2879..2881,aa:Ser,seq:cga)
     gene            2969..3054
                     /locus_tag="VF276_06300"
     tRNA            2969..3054
                     /locus_tag="VF276_06300"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3004..3006,aa:Ser,seq:gga)
     gene            3171..3932
                     /locus_tag="VF276_06305"
     CDS             3171..3932
                     /locus_tag="VF276_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606511.1"
                     /translation="MDNENTDMGAGQVSPEEALRQLVQLRVQLGPARFNSVARQMGMP
                     DELLDHIEQTVALMGDTEGGANGPAPSGLVDASGRPISSGNAGGLVDATGRPLTPSAP
                     SLDGLNGSAAGEDEDADEEGLEGIELDEESSRQLFQELVGVRMIIGPENFTNLAQQMG
                     MPTEASEMVEVAAGQVEEEIAGRYENQEEAMTVRLVNARMMMGQERFADFAGQVGMPA
                     EVIDEVESIAGQIESGAESADDDGEDDEDEGPSLA"
     gene            4076..4337
                     /gene="ffs"
                     /locus_tag="VF276_06310"
     ncRNA           4076..4337
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VF276_06310"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            4406..>4810
                     /locus_tag="VF276_06315"
     CDS             4406..>4810
                     /locus_tag="VF276_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002725333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HEX6606512.1"
                     /translation="MDSPAPEIRPDALDARAVAAHLRAAALAAVDPARAVAAALARDA
                     PAGTLTVAGHPLALDQFDRVFALGAGKAGASMAQAAEAALGDLPTWQGGLVIVKDPPP
                     RPGPATIELVQAGHPLPDARGVEAAQRIGALAR"
BASE COUNT          794 a         1582 c         1647 g          787 t
ORIGIN      
        1 tttgcaactc acggcgaagc gtaagaaccc gccaaatacc cctgcgcgca agaccgagca
       61 gggctgcacg caggggtgaa aggccggcgg gcgggccggg cgccggcccg gtgggaccta
      121 cccgctcccg cccaactgcg cggccagcgc ggcggggtcg gtcacgggcg actggcaggc
      181 gtagttgacg cagacgtagg cggtggcctt gccgtcgcgc tgcgggcggc cttcgagcag
      241 cggcacggcg gcgatggccg ccgaatcgtc ggcggcgccc gcggccacca cctggtgggg
      301 ccggtagccc tggaacacga cggcgagcag cgcggcggta tcggcggcct ccgggcgccc
      361 gatcagcgcg atctcctgga cctcggccag gtagaggtcg agcacgcaga gcatccgccc
      421 caccccgctg ggatactggg ccagcagcgc gcccgccgcc tccagcgcgc cgcgcgccgc
      481 gtcgtagtag tcctcttcca tcgtatagta atggagccgc agcagcacct ccgccgccgc
      541 cgaattgccc gacgggatgg cgctctcgaa caggtccttg ggccggttga tcaactgctc
      601 gccgtcggcg ggcgtgtcga agaacccgcc gcgctcggtg tcggcgaact gggcgagcag
      661 cgtggcggcc aggctgcgcg ccgccaggaa ccagtgcggg tcgaagtcgg cctcgtagag
      721 cgccagcagg ccggcggcgt agaaggcgta gtcttccagg aacccggcca ggtgcgcccg
      781 cccgtccttg taggtgcgcc acaggcggtc gccgtcgagc atggtgccca gcacgaaggc
      841 ggcgttgtgc tgggccacct ggcggtagtc ctcgcggccc agcacccgcg ccgcctcggc
      901 gaagctgcgc agcatcaggc cgttccacga gaccagcacc ttgtcgtcgc gccccggcca
      961 gacgcgctgc gcccgcgcgg catagagctt gccccgcgcg gcggccagca aggccggcac
     1021 cgtatcgagc ggcagattca ggtcctgggc cacggcatcg gccgggcgcg gcacattgag
     1081 gatggtcttg ccctcgaagt tgccctgctc ggtcacgccc cagtaggcgc gggccacgcg
     1141 ggcctcctcc ggcgtgagca ccgcgtcgat ctcatccggg gtccagacat agaacttgcc
     1201 ttcctcgccc tcgctgtcgg cgtcctgcgt ggagtagaat gcgccctccg ggctggtcat
     1261 ctcgcgcacc acgtagtcca gcacctcttc gacgatgcgc cggtagaacg gctcgccggt
     1321 gatctggtag acgtgcaggt agagccggct gagcagcgcg ttgtcataga gcatcttctc
     1381 gaaatggggc accagccagt ggttgtccac cgaatagcgg tgaaagccgc cgcccacctg
     1441 gtcgtagatg ccgccgttcg ccatggcgcg caacgtctgc tcgatcatcg ccagcaggcg
     1501 ggcgtcgcgg gtgcggtggt actggcggac caggaactcc caggccatcg gctggggaaa
     1561 cttgggcgcg ccgcccaggc cgccgtggcg gctgtcgaac tccccggcca gcgcggcggc
     1621 cgcccggtcg aggaactccg ggcgcagggc ttcgggcgcg gcggggatgg cgctctgttc
     1681 ggccaggtgc gcggccaggt cgtccgccga ctggtcgatc tcgccgcgct gctgctcgta
     1741 ggcgcgggcc accgattgca gcacgcgctt gaagccgggc aagccatggc ggtcgtcggg
     1801 cggaaagtag gtgcccgcga aaaacggctt gcccgcgggc gtcaggaaca ccgtcatcgg
     1861 ccagccgccc tgcccggtca gggtttgcac cgccgccata taaatcgagt ccaggtcggg
     1921 ccgctcttcg cggtccactt tgatggacac aaagtgctga ttcatcaggg ccgccgtggc
     1981 cgggtcctcg aacgactcgt gctccatcac gtggcaccaa tggcacgccg agtagccaat
     2041 cgacagcaga atcggcttgt cctcggtgcg ggcgcgggcc agggcctccg gcccccaggg
     2101 gtaccaatcc accgggttgt gggcatgttg caacaggtaa gggctggttt cttgactcaa
     2161 gcgattagcc atggcgtgat cctctctaat gcgcaacggt cgccggccag gcgggccggt
     2221 atagccgagt gcccgcaagg actatgccaa ctttcttaac gtgttgaaat caagatttaa
     2281 ccaaaggtac tattgtgttc tcttaaaagt ccggatatga gaatagtagg cgtgattcca
     2341 cgccgcgtga gttggcccgc tgccagcgcg tgagttggca cactgacagc agcattggac
     2401 gttctgggag ccggggacat gaaaaaggat agtcgcttac gggcgcatgc cgcgcgcgcg
     2461 caagggctgg tggagtacgg gctgatcctg gtcttagtca tggtcgtcgt agtcgtggtc
     2521 gtcgccctcg ccggcgatga tctcaacacg atgttttccg tcgtcgccag cgaggtgctg
     2581 gctattaccc cctgagccac gcctgcaacc gcgccgcgcc acgctgcatg ggtcccaaat
     2641 ataattaaat cgccattcat ttgccatcta tacctgattt ttccttgcat aattcgggac
     2701 cttgtggtat aatttccccg taagcacgcg gccgtagctc agcggattag agcgttgggt
     2761 tgcggaccca aaggtcgtag gttcgagtcc tatcggccgc gccacagccg acctattaac
     2821 tcgttaatgg gtcggctttt ttatggagag gtgtccgagt ggttgatgga gctcgtctcg
     2881 aaaacgagtg aggtgtgaaa gccttcgtgg gttcgaatcc caccctctcc gccccgccgg
     2941 gcggtcacaa gtaaagaggt ttgtgtctgg agaggtcgcc tagttggcct atggcgctcg
     3001 cctggaaagc gggtacggtg caagccgtcg tgggttcgaa tcccaccctc tccgccctcg
     3061 tcggctgtgg gtgagcccct ggagggttcg cccacttttt attgcccggt ctggtcgccg
     3121 gccgttgtgc cgcgatccgt atgccggcga ccagcagtaa aggagaaacc atggataacg
     3181 aaaacaccga catgggtgcc gggcaggtca gtccggaaga ggcgctgcgc cagctggtgc
     3241 aactgcgggt gcaactgggg cccgcgcgct tcaacagtgt ggcccggcag atgggcatgc
     3301 ccgacgagtt gctggaccat atcgagcaga ccgtggccct catgggcgac acggagggcg
     3361 gggcgaacgg cccggccccc agcggcctgg tcgacgccag cggccgcccg atcagcagcg
     3421 gcaacgccgg gggcctggtg gatgccaccg gccgcccgct cacgcccagc gcgcccagcc
     3481 tcgacggcct caacggcagc gccgccggcg aggacgagga cgcggacgag gagggcctgg
     3541 agggcatcga gctggacgag gagtcgagcc gccagctctt ccaggaactg gtcggcgtgc
     3601 gcatgatcat cggccccgag aacttcacca acctggccca gcaaatgggc atgcccaccg
     3661 aggccagcga gatggtcgag gtcgccgccg ggcaggtcga agaggagatc gccggccgct
     3721 acgagaacca ggaagaggcc atgacggtcc gcctggtcaa cgcgcgcatg atgatgggcc
     3781 aggagcgctt cgccgatttc gccggccagg tgggcatgcc cgccgaggtc atcgacgaag
     3841 tcgagagcat cgccgggcag atcgaatccg gcgcggagag cgcggacgac gacggcgagg
     3901 acgacgagga cgaagggccg tccctggcct gatccgccgg ccccccgcca agcacgaagc
     3961 acgcgaagac gattgctgtt agcgtcattc ttcgcgtgct tcgccgcttt ccggcgtatc
     4021 gagatgccgc cgctcgcctt gacatttggc ggcgattcga gtatgattac ctcagtcgtg
     4081 ctaagcgggg agctagcgct accctgtacc cgcaatgcgc tttagcgggg ccgaattccc
     4141 aatcgaggtc cggctttgta ggactgctgg tacgaagtgg tgttgaaagt cgggtcctac
     4201 gcggcagaac actatgaacc ctgtcaggac cggaaggtag cagcagtaaa tagaccgttc
     4261 tgggtgccgt aggggtgcct ggctggagct aactggtatc agtaagctgg aaggctgtat
     4321 cgacaacggg tgcacgattt gctatcattg gagggttgcc caaccgggca gccctctttt
     4381 gctatcgccg taggggagac cggccatgga ctcgcccgcg cctgagatcc gccccgatgc
     4441 gctcgatgcc cgcgccgtgg ccgcgcacct gcgcgccgcc gccctggccg ccgtggaccc
     4501 ggcccgcgcc gtcgccgccg ccctggcccg cgatgccccc gccggcacgc tgaccgtggc
     4561 cgggcacccg ctcgccctcg accagttcga ccgtgttttc gcgctgggcg cgggcaaagc
     4621 cggcgcgtcc atggcccagg ccgccgaggc cgcgctgggc gatctgccca cctggcaggg
     4681 cggcctggtc atcgtcaagg acccgccgcc ccggcccggc ccggccacca tcgaactggt
     4741 gcaggccggg catcccctgc ccgatgcccg cggcgtcgag gcggcgcagc gtatcggcgc
     4801 gctggcccgc
//
LOCUS       DASUYC010000382         3439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_20459,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000382 DASUYC010000000
VERSION     DASUYC010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20459"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1514
                     /locus_tag="VF276_06320"
     CDS             <1..1514
                     /locus_tag="VF276_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606513.1"
                     /translation="VQYFERNRFELHPDDPHGNTVLLGLLGTLLTDGRTFPTSDPVPE
                     TSDQKYFVQTGHRLSGVFYRYWNTHGGLAIFGYPISEEMQEGDYKVQYFQRNRFEYHP
                     EHAGTPYEVELGLLGRDYLSMVQSGLVAVPGGPAKPTSDPNNPAPPITAGPTMVPQDG
                     AFLKGPHIGYGMIVHMYYQDHNRILNAVKDLGFTWIKQQVQWSDTENPKGTYYWGELD
                     RIVNDTHAAGLNLMLSIVRTPQWARSSGVGFPDRPQDLADFMQAMANRYAGRVQAYEI
                     WNEENLGNETGPDHWHPAKYAEILKAGYLGVKAGDPTAIVISGALTPTGVYTDDAVED
                     TWFLDQLYQWNNGEIRKYYDVLGMHPYGYWNPPDAMYPVRQPSDQTPATEYKNHGSFY
                     FRRIEDQRAVMEKHGEGQKQVWLTEFGWCSDQRDGGYVECINNSLQDQANYIVRAYQK
                     AETDYPFMGVMFLWNLNFSTFQEWYTGPSHFAILNPDWSPRPAYWALKNMPKP"
     gene            1649..2305
                     /locus_tag="VF276_06325"
     CDS             1649..2305
                     /locus_tag="VF276_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606514.1"
                     /translation="MLRFLVLFLLLAGGLIACDSGTGPQPSVPTGTPVSVPTGTLVSV
                     ATDTATVVSLPPAVATDSAKNTQVVTYRIHVATSVALTGEPTWTPGAPPDLPTLTPIL
                     GIVGGCPSPNSRGFQCYNMWRGYHAGHLMEVRVGRQGSDEDPTQGEIVVLTRGTRETY
                     FGPGKTGAFDIVSLDGDLVTVTAVESPHHEIFIFNLQTHQWTTISPLPGGSPTASPLP
                     "
     gene            complement(2901..>3439)
                     /locus_tag="VF276_06330"
     CDS             complement(2901..>3439)
                     /locus_tag="VF276_06330"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEX6606515.1"
                     /translation="TVAPTRTTGAGLALPAPPSRTAAGAGTATVAPARTVAAIVSRVV
                     SGDTLDVMIGAEPARVRLIGVEAGGDAAPPGGTCFGPEATAHVQQLVVQAGRQVLLEK
                     DVSEADATGRLLRYVWLPGAGAPVLLNEDLAGQGYAQVRPTPPDGKYQDRLRAAQQAA
                     QAQHRGRWAVCPPTTPQR"
BASE COUNT          628 a         1188 c         1070 g          553 t
ORIGIN      
        1 aggtgcagta tttcgagcgc aaccgctttg aactgcaccc cgatgatccg cacggcaaca
       61 cggtcctcct gggcctgctg ggcacgctgc tgaccgacgg acgcaccttc ccgaccagcg
      121 acccggtgcc cgaaacgtcc gaccagaaat acttcgtgca gaccggccat cgcctcagcg
      181 gcgtgttcta ccgctactgg aacacccacg gcgggctggc gattttcggc tatcccatca
      241 gcgaggaaat gcaggaaggc gattacaagg tccagtattt ccagcgcaac cgctttgagt
      301 accacccgga gcacgccggc accccctatg aggtcgaact gggcctgctg gggcgcgact
      361 acctgtccat ggtgcagagc gggctggttg ccgtgcccgg cggcccggcc aagcccacgt
      421 cggaccccaa caatccggcg ccgccgatca ccgccgggcc cacgatggtc ccgcaggacg
      481 gcgccttcct caaggggccg cacatcggct acggcatgat cgtgcatatg tattaccagg
      541 accacaaccg catcctcaac gcggtgaagg acctgggctt tacctggatc aagcagcagg
      601 tgcagtggag cgacaccgaa aaccccaaag gcacctacta ctggggcgag ctcgaccgta
      661 tcgtcaacga cacccacgcg gcgggcttga acctgatgct gagcatcgtc cgcacgccgc
      721 aatgggcgcg tagctccggc gtgggcttcc ccgaccgccc gcaggacctg gccgacttca
      781 tgcaggccat ggcgaaccgc tatgccgggc gcgtccaggc ctacgagatc tggaacgagg
      841 agaacctggg caacgagacc ggccccgacc actggcaccc ggccaagtac gccgagatcc
      901 tcaaggcggg ctacctgggc gtcaaggccg gcgatcccac cgccatcgtc atcagcggcg
      961 cgctcacgcc caccggcgtc tacaccgatg atgcggtaga ggacacctgg ttcctggatc
     1021 agctttacca gtggaacaac ggcgagatcc gtaagtatta cgacgtgctg ggcatgcacc
     1081 cctacggcta ctggaacccg cccgacgcga tgtaccccgt gcgccagccg tcggaccaga
     1141 ccccggccac cgagtataag aaccacggct cgttctattt ccgccgcatc gaggaccagc
     1201 gcgcggtcat ggaaaagcac ggcgagggcc agaagcaggt ctggctgacc gagttcggct
     1261 ggtgcagcga ccagcgcgat ggcggctacg tcgagtgcat caacaactcg ctacaggacc
     1321 aggccaacta tatcgtgcgc gcctaccaga aggcggagac cgactacccg ttcatgggcg
     1381 tgatgttcct ctggaacctc aacttcagca ccttccagga gtggtacacc ggcccgtcgc
     1441 acttcgcgat cctcaacccc gactggtcgc cccgcccggc ctactgggcg ctgaagaaca
     1501 tgccgaagcc ataatctacg accttgccca acaccggacc gggagcgcgc cgctcccggt
     1561 cccctggcgg tagttggggc caccggcaat taggcgcgtc gagagcagga ccaattcgca
     1621 ggcaaatata gctgaacagg ggccgccagt gttgcgtttt ctcgtgctat tccttctcct
     1681 ggccggtggg ctgatcgcgt gtgactccgg caccggcccg caaccgagcg tcccgaccgg
     1741 cacgcccgtg tccgtcccga ccggcacgct cgtgtccgtc gcgaccgata cggcgaccgt
     1801 cgtctccttg ccacccgcag tagctaccga tagcgccaaa aacacgcagg tggtcaccta
     1861 tcgcatccac gttgccactt ccgtcgcgct tactggggag ccgacctgga cgccgggcgc
     1921 tccgcccgac ctgcccaccc tcacccccat actcggcatt gtcggcggct gtcccagccc
     1981 gaattcacgc ggcttccaat gctataatat gtggcgcggg tatcacgccg gccatctgat
     2041 ggaagtgcgc gtgggccggc agggttcgga cgaggatcca actcaggggg aaatcgttgt
     2101 gctgacgcgc ggtacccgcg agacctattt cgggccaggg aaaacaggtg ctttcgacat
     2161 tgtgtctctc gacggcgatc tcgtgacggt aaccgctgtc gaatccccgc accatgagat
     2221 cttcatcttc aatctccaga cgcaccagtg gaccaccatc tccccgctgc cgggtgggtc
     2281 gcccaccgcc agccccctgc cgtaaccggc gccccggcag gcacgcgaag cgcgttcagg
     2341 gctaacggca gccgtgaaat taatcccgtt gctaggcact tccgcggcac aggaacggac
     2401 cgggcgcttg acgttgcggg ggcaatgtgg taatataatg acgcagcccc gccacgcggg
     2461 gacgtcacaa gaaagtcttg atccaacagt tttgaacagg gagcttgtag cggatgggga
     2521 cgcctgtggc cggcttgtcc gattgaggcg gtaacctgaa gaagagcacc cacctgcaca
     2581 tgtgttgcag gttcaagacc taagtggcgc cccctcgtgg cggggttcgc gcatatcccg
     2641 cacctgacag ggccgcaacg gtagaggacc gatcctccca cgttgcggcc cttttgttgc
     2701 ccggcgccgc cctcgtgttc cttgtcactc tgagcgcagc gaagagtctc ggcgcgtggg
     2761 caaggagacg agatgcttca ctgcgttcag catgacaacg ggggcttgtc actctgagcg
     2821 cagcgaagag tctcgtctcg cgggacagga gacgagatgc ttcactgcgt tcagcatgac
     2881 aacgggggtg ggaaggattt tcaccgttgc ggcgtggtcg gagggcagac ggcccagcgg
     2941 ccccggtgct gcgcctgggc ggcctgctgg gcggcgcgca ggcggtcctg gtacttgccg
     3001 tccggcgggg tggggcgcac ctgggcatag ccctgcccgg ccaggtcttc gttcaacaac
     3061 accggcgccc cggcgcccgg caaccagacg tagcgcagca gccgccccgt cgcgtcggcc
     3121 tcggacacgt ctttttcaag cagcacctgc cgcccggcct ggaccaccaa ctgctgcacg
     3181 tgagcagtcg cctccggccc gaagcatgtc ccaccgggcg gagccgcatc gccgcccgcc
     3241 tcgacgccga tcagccgcac gcgcgccggc tccgccccga tcatcacgtc gagcgtatcc
     3301 ccgctgacga cccggctgac aatcgccgcc accgtgcggg cgggcgcgac ggtggcggtg
     3361 ccggcgccgg cggcggtgcg cgacggcggc gcgggtagcg ccagcccggc ccctgtcgtg
     3421 cgggtgggcg cgaccgtga
//
LOCUS       DASUYC010000383         2050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1162034, whole genome shotgun sequence.
ACCESSION   DASUYC010000383 DASUYC010000000
VERSION     DASUYC010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162034"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VF276_06335"
     CDS             <1..411
                     /locus_tag="VF276_06335"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX6606516.1"
                     /translation="INPAAVACAAGNVRRYGLQDRVVVRAGDLFAPVADEWFDLILCN
                     PPYFQGTPHTLADAAFLGGPQYEWLDRFAAEAGAHLAPGGRILVVLGDAADVPALLAR
                     LAAPGWRVSEVARRDLWMEMLYIYALTPRLEAAG"
     gene            408..1565
                     /locus_tag="VF276_06340"
     CDS             408..1565
                     /locus_tag="VF276_06340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX6606517.1"
                     /translation="MSRAGGVLAAIMLVGLAIRLYGLTSYGIWFDESYHIALVQLPGV
                     GAMLDAVLSNPPSDPLYVLLLRPWVALFGHGDGSVRALSVGLSTATLPATYWLGRSLV
                     GPRAGLLGAALLAVSPYAVELGQEAALYALAALTTTLALAAGWHWWRTGQGARRYLAL
                     GIIAIYSHYVVAAILAGFALLVLLPGTGPRQVSRRAWLTGHAIIGATWLPWLAALAIH
                     WQQSAVPRATLQHRAGLADVRDALIQYTSGTAAMLHGPRALQVLGLLAGAALLLGGWL
                     AGGDPHRRGLRLLLLLSGVVFLAPAVVSFLTGRWLFVPHFMLFLLPAIFVVLAASYQP
                     SAVSRVPAAPLRDQVKACEATPSGGLRASPAGRGGPAWLTGPIWYCPADIV"
     gene            1633..>2050
                     /locus_tag="VF276_06345"
     CDS             1633..>2050
                     /locus_tag="VF276_06345"
                     /inference="COORDINATES: protein motif:HMM:NF014880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HEX6606518.1"
                     /translation="MFRFFRRQKQEQPAPEAEAPQVAEVAPVPVPAPPSAPATNEAPA
                     RVAPMAPVPPVAQNVALEREIEEEEREQKQKVAKSLQPTRRGFFSQITELFSADEITS
                     SLWDDLQDLLVLGDCGVDTTNYVLDELEKRVNRERIR"
BASE COUNT          257 a          726 c          716 g          351 t
ORIGIN      
        1 atcaatcccg ccgccgtggc ctgcgccgcg ggcaacgtcc gccgctacgg gctccaggac
       61 cgcgtggtcg tccgcgcggg cgacctgttt gccccggtcg cggatgagtg gttcgacctg
      121 atcctctgca atccgcccta cttccagggc acgccgcaca ccctggccga cgctgctttc
      181 ctgggcggcc cgcagtacga atggctggac cgcttcgcgg cggaggcggg cgcgcacctg
      241 gcgccgggcg ggcgcatcct ggtcgtgctg ggcgacgccg ccgacgtgcc cgcgctgctg
      301 gcccggctcg ccgcccccgg ctggcgcgtc agtgaggtcg cccgccgcga cctctggatg
      361 gagatgctct acatctacgc cctcacgccc cgcctggagg ccgcgggatg agccgcgcgg
      421 gcggggtgct ggcggcgatc atgctcgtgg gcctggcgat ccggctctac ggactgacca
      481 gctacggtat ctggttcgac gagagctacc atatcgccct ggtgcaacta cccggtgtgg
      541 gcgccatgct cgacgccgtg ctgtcgaacc cgccgtcgga cccgctctat gtgctgctgt
      601 tgcggccgtg ggtggcgctg ttcgggcacg gcgacggctc ggtgcgtgcc ctgtccgtgg
      661 gcctgagcac ggccacgctg cccgccacct attggctggg ccgctcactg gtcgggccgc
      721 gcgcgggctt gctgggcgcg gcgctgctcg ccgtctcgcc ctacgccgtg gaactgggcc
      781 aggaggcggc gctgtacgcc ctggccgcgc tgaccacgac gctggccctg gccgcggggt
      841 ggcactggtg gcgcacgggg cagggcgcgc gccgctacct cgctctgggc atcatcgcga
      901 tctacagcca ttatgtcgtc gccgccatcc tggcggggtt cgccctgctg gtgctgctgc
      961 cggggaccgg cccgcggcag gtatcccggc gcgcctggct caccgggcac gcgattatcg
     1021 gcgcgacgtg gctgccctgg ctggcggccc tcgccatcca ctggcagcag agcgccgtac
     1081 cccgcgccac gctgcaacac cgcgccggac tggccgatgt gcgcgacgcg ctgatccagt
     1141 acaccagcgg cacggcggcg atgctgcacg ggccgcgggc actgcaggtg ctgggcttgc
     1201 tggcgggtgc cgcgctgctg ctcggcgggt ggctggcggg gggcgatcct caccgccgtg
     1261 gcctgcgcct gcttctcctg ctgagcgggg tcgtcttcct ggcgcctgcc gtcgtctcgt
     1321 tcctgaccgg ccgctggctt ttcgtccccc atttcatgct gttcctgttg ccggcgatct
     1381 tcgttgtcct ggcggctagc tatcagccgt cagccgtcag ccgtgtgcct gcggcaccgc
     1441 tgcgcgatca ggtaaaagca tgcgaggcca ccccatcggg tggccttcgc gcatcacccg
     1501 caggtagggg cggaccggcg tggctgaccg gccccatttg gtattgccct gcggatatag
     1561 tataatttat ggggcttgct gcccccggac ttttcgcgtc ctatggacgc tttctcagga
     1621 gccaaacaac gagtgtttag atttttccgc cgccagaagc aggaacagcc tgcccccgag
     1681 gccgaggcgc cgcaggtggc tgaggtggcg ccggtccccg tgcccgcgcc gcccagcgcg
     1741 cctgccacta acgaggcacc tgcgcgtgtg gcgcctatgg cgcccgtgcc gccggtcgcg
     1801 cagaacgtgg cgctggagcg cgagatcgag gaagaggagc gcgaacaaaa gcaaaaggtt
     1861 gccaagagcc tccagccgac ccggcgtggc ttcttcagcc agatcaccga actcttctcg
     1921 gcggacgaga tcaccagcag cctgtgggac gacctgcaag acctgctggt gttgggcgac
     1981 tgcggcgtcg ataccaccaa ctacgtgctg gacgagctgg agaagcgcgt caaccgcgaa
     2041 cgcatccggc
//
LOCUS       DASUYC010000384         3242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_693965,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000384 DASUYC010000000
VERSION     DASUYC010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693965"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /gene="trxA"
                     /locus_tag="VF276_06350"
     CDS             <1..445
                     /gene="trxA"
                     /locus_tag="VF276_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX6606519.1"
                     /translation="ETVRIMQMGEKASMVKPSQDTGPAAQSPIVEVSDETFVAEMLDA
                     ELPVLVDFMAQWCGPCKMMGPIFAEAAVEYVGRVKFVKVDVDACPQVAGALQIRSIPT
                     FALFAGNQAVAAGVGAMRAADLRRWIDEALTRVPAQDAASAPEPA"
     gene            449..1312
                     /locus_tag="VF276_06355"
     CDS             449..1312
                     /locus_tag="VF276_06355"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6606520.1"
                     /translation="MGPSGTPSPTTAAEQWATALGTWAIPPAILAAAPESPWGYAPAL
                     FARRAEAALAQHTPSADIAQAALPAGGAVLDVGCGAGAASLPLAGPAALLVGVDPSPE
                     LLAIFEERARALGVRVLALRGQWPAIAAETPVADVVVCHHVAYNVADLAPFVVALTRH
                     ARRRVVLELTARHPLSDLNDLWLRFHGLVRPQEPGADDAVAVLRELGLAPERRDWTAA
                     GSGVGDGFATPDDLVAWTRRRLCLPVLRDPEIAATLAEQLGPDPATWQLPPRRLVTLW
                     WPGGAGSAAAP"
     gene            1552..2307
                     /locus_tag="VF276_06360"
     CDS             1552..2307
                     /locus_tag="VF276_06360"
                     /inference="COORDINATES: protein motif:HMM:NF021619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
                     /protein_id="HEX6606521.1"
                     /translation="MTELLTVRDVQDLLKVDRITVYRMLKDGRLTGVKIGAHWRFARP
                     QLDALVTAPRTPAHPPLPALGQALPLHCMQLIQDVFAEVGEVSVITTTPAGEPLSTLS
                     RPGPFWKLLQTSERGRQDALATWRRIAGDGPQPDQFVTCQAELDYTSAPVIVDGMPVA
                     VVIAGPFYATPPDPHEEAVRWAGLARSYHLDPGALRAAARQIPVLDARKRAQIPAWVQ
                     NVATTFAEMSRERAALVTRLRSIAAMTNFDTAS"
     gene            2401..2814
                     /locus_tag="VF276_06365"
     CDS             2401..2814
                     /locus_tag="VF276_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009059885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HEX6606522.1"
                     /translation="MSEKFSIVLFSGTVDKIMAATTMIAGAAAMGKEVTLFLTFGGLI
                     AFRKDAWQTNMQFSADFAPYAGPAMELMQVKQVPHWMTTLQQAMEVGNVKVKACGMTM
                     DLFNIKLTDMEPIVSEVTGVASFIEESDGGTTLFI"
     gene            2849..3088
                     /locus_tag="VF276_06370"
     CDS             2849..3088
                     /locus_tag="VF276_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEX6606523.1"
                     /translation="MDTIMITKEIDARGSFCPGPLMALIGAVKVAQIGDVLAVISSDE
                     GSKKDIPAWIAKAKQDYLGAEPVGTATRFVCRKVK"
BASE COUNT          553 a         1091 c         1069 g          529 t
ORIGIN      
        1 ggagacagtc aggatcatgc agatgggaga aaaggcatct atggttaagc cgtcgcaaga
       61 caccgggcct gccgcgcaga gcccgatagt cgaggtttcc gacgaaacct ttgtggccga
      121 aatgctggac gcggagctac cggtcctcgt cgatttcatg gcccagtggt gcggaccatg
      181 caagatgatg gggcccatct tcgccgaggc cgcggtcgaa tacgtgggcc gggtgaagtt
      241 cgtgaaggtc gatgtggacg cctgccccca ggtggcgggc gccctgcaaa ttcgcagcat
      301 cccgacgttc gcgctattcg cgggcaatca ggccgtcgcg gccggcgtcg gcgccatgcg
      361 cgccgctgac ctgcgccgct ggatcgacga ggcgctgacg cgggtacctg cgcaggacgc
      421 ggcgtccgcg ccggaaccgg cgtaggctgt ggggccatcc gggactccgt ctccgacgac
      481 agccgccgag cagtgggcta cggcgctggg cacctgggcc atcccgcccg cgatcctcgc
      541 ggcggcgccg gagtcgccct ggggatacgc gcccgcgctc tttgcccgcc gggcggaggc
      601 ggcgctggcg cagcacaccc cgtcggcgga tatcgcgcag gcagccttac cggccggcgg
      661 cgctgtgctc gacgtaggct gcggcgccgg ggctgcctcc ctgcccctgg ccgggccggc
      721 ggcgctgctg gtgggagttg atccctcgcc ggaactactg gcgatcttcg aggaacgcgc
      781 gcgcgcgctc ggtgtgcgcg tcctcgcgct gcgcggccag tggcccgcga ttgccgccga
      841 aacccccgtc gccgatgtgg tcgtctgcca tcacgtcgct tacaacgtcg cggatttagc
      901 gccgtttgtc gtcgcgttga cccggcatgc ccggcgccgg gtggtgctgg agctaaccgc
      961 ccgccatccg ctgagcgact tgaacgacct gtggctgcgc ttccacggcc tggtccgtcc
     1021 gcaagagccg ggcgctgatg atgcggtcgc ggtgttgcgc gaactgggat tggcgcccga
     1081 acggcgggat tggactgcgg cggggagcgg cgtgggagac gggttcgcca cgccggacga
     1141 cctggtggcc tggacacgcc gccgcctgtg cctgccggtc ctgcgcgacc cggagatcgc
     1201 cgcgacattg gccgagcaac tgggcccgga tcccgccacc tggcagcttc cgccccggcg
     1261 cctggtgacg ctctggtggc cggggggcgc ggggtccgcc gccgcgccct gagaggcgct
     1321 gcgccgagcg catcggactc cggcaataac ctccacgcga agccggcgcc gtcatgcacg
     1381 gcccttgccc cctgatcccg atcctctttc ctggcgatct ccagcatcac ctcctccaag
     1441 tcgatatgta tatattttca caaagttgat acgcaaatct ttacctcgcc gagtcatact
     1501 ttgttataga tagctatagc acgctaacgc cgcgggccga ggagaggagc gatgaccgaa
     1561 ctactgactg tgcgggatgt gcaagatctg ctcaaggtcg atcgcatcac ggtctatcgc
     1621 atgctcaagg acgggcgctt gaccggcgtc aaaatcggcg cacactggcg cttcgcccgg
     1681 ccgcaactcg atgccctggt gacggcgccc cgcactccgg cccatccccc gctgccggcg
     1741 ctcggccagg cgctacctct acactgcatg caactcatcc aggacgtctt tgccgaggtc
     1801 ggggaggtga gcgtgatcac caccacccct gccggcgagc cgctcagcac actcagccgg
     1861 ccgggcccct tctggaaact ccttcagaca agcgagcgag gccggcagga tgccctggcc
     1921 acctggcggc gcatcgcagg cgacggtccc cagcccgacc aattcgttac ctgtcaggca
     1981 gaactcgatt acacgagcgc gccggttatc gttgacggca tgccggtcgc ggtggttatc
     2041 gcgggcccat tttacgccac cccgcccgat ccacacgaag aagccgtccg ctgggccggc
     2101 ctggcccgca gctatcacct cgaccccggc gccttacggg ccgccgcgcg gcagatcccg
     2161 gtgctcgatg cccgcaagcg cgcgcagatc ccggcgtggg tgcaaaacgt cgcgactacg
     2221 ttcgcggaga tgagccgcga acgcgccgcc ctcgtgaccc ggctgcgtag catcgcagcc
     2281 atgaccaatt tcgacaccgc ctcctgatcg gtgggaccgc ctccggcggg cttgggtgag
     2341 cgggacaatt gcgcaaacga gccatccctg cgcttttcgg tttttgaaag gggttcggag
     2401 atgagcgaga agttttccat cgtgctgttc tcaggcaccg tggacaagat tatggcggcc
     2461 acgaccatga tcgcgggcgc cgcggcgatg ggcaaagagg tgaccctatt cctgaccttc
     2521 ggcggcctga tcgccttccg caaagacgcc tggcagacca acatgcagtt cagcgccgac
     2581 ttcgcgccgt atgccggccc ggcgatggag ctaatgcaag tcaagcaagt gccccactgg
     2641 atgactaccc tgcagcaagc gatggaagtt gggaacgtca aggtcaaggc ctgcgggatg
     2701 accatggacc tgttcaacat caagctgacc gacatggaac cgatcgtcag cgaagtgacg
     2761 ggcgtggcca gcttcatcga agagtccgat ggtgggacaa ccctgtttat ctagccgcgc
     2821 caggcagcgc cggcaaggag tatacatcat ggacacgatc atgattacca aagagatcga
     2881 cgcgcgcggc agcttctgcc ccggcccgct catggccctc atcggcgccg tgaaggtcgc
     2941 ccagatcggc gatgtgctcg ccgtcatctc cagcgacgag ggcagcaaga aggacattcc
     3001 ggcctggatc gccaaagcca agcaagacta tctcggtgcc gagccggtgg gcaccgcgac
     3061 gcgcttcgtg tgccgcaaag tgaagtaacc aggcggatgg gcccggcggc gcccgtggcg
     3121 ccgccagcca aaggagatga ccgcaatgaa gcgcatcgta attctgggtg ggggcacggg
     3181 cggcaccctg atcgccaacc tgctggcgca gaaactccgc ccgaccgagg ccgaaatcct
     3241 gc
//
LOCUS       DASUYC010000385         2255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_306850,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000385 DASUYC010000000
VERSION     DASUYC010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306850"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1426
                     /locus_tag="VF276_06375"
     CDS             <1..1426
                     /locus_tag="VF276_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX6606524.1"
                     /translation="KDPDEVLVDIGSKSEGIIPSNELSSLTSEEFARLAIGDEVLVSV
                     LQPEDKEGHVVLSLDRARQEKSWRKLQKQFENNEIIEAEVNGYNKGGLLVNLEGVRGF
                     VPASQVTGLSSGGTDAAKQSELSRFTGQKLPLKIIEINRNRNRLILSERQAAQEQRDA
                     RKEQLLAELQPGQIREGHVSSICDFGAFVDIGGADGLIHLSELSWSRVNHPSEVLKVG
                     QPVRVYVMSVDERDRKIALSLKRTQPEPWSQVMEHYQLGQLVRGTITQLTDFGAFARL
                     EDGIEGLIHVSELAEGRVTHPRNVVQEGWELTLRVIRIDPQRRRMGLSLKRAMEPQEG
                     DTLPPQEPLPVPTPRAAGEGDQGGRGGRGGQGGRGGANRGRGREEEQAGGGEATAQAE
                     APPPQPQPQPVAPAPEPDLGDTAMAQAMTAARAAAASETAEAEAPVNNAEPAGSASDE
                     AEMGAGENETPAGEAEGDDTGTAT"
     gene            1692..>2255
                     /locus_tag="VF276_06380"
     CDS             1692..>2255
                     /locus_tag="VF276_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX6606525.1"
                     /translation="MAERFDKFTERARKVLTLAQEEAQRFNHNYIGTEHILLGLVREQ
                     EGVAARVLSNLGIELPKVRSAVEFIIGRGDRIVMGEIGLTPRAKKVIELAVDEARRLN
                     HHYIGTEHLLLGLVREGEGIAAGVLESLGVSLEKVRTQVIQVLNSSGQQYGQKQSGPS
                     KTPYVDALGVDLTAQAAAGKLDPVIGRK"
BASE COUNT          441 a          724 c          781 g          309 t
ORIGIN      
        1 caaggacccc gacgaggttc tggtcgatat cggatcgaag tcggagggca tcatccccag
       61 caacgaattg tcgagcctta cgtcggagga attcgcgcgc ctggcgatcg gcgatgaagt
      121 gctggtgtcg gtcttgcagc cggaagataa agaaggccat gttgtgcttt ccctggaccg
      181 ggcacgccag gagaagtcgt ggcgcaagct gcagaagcag tttgagaaca acgagatcat
      241 cgaggcggaa gtcaacggat acaacaaggg cggcctgctg gtgaacctgg aaggtgtgcg
      301 cggcttcgtg ccggcgtcgc aggtcacggg cttgagcagt ggcggcaccg atgccgccaa
      361 gcaaagtgaa ctctcgcggt tcactggtca gaaactgccg ctcaagatta tcgagattaa
      421 ccgcaaccgc aaccggctga tcctgtccga gcggcaggcg gcgcaagagc agcgcgatgc
      481 ccgcaaggag caactgctcg ccgaactgca gccgggccag atccgcgaag gtcatgtgtc
      541 gagcatctgc gatttcggcg cctttgtcga tatcggcggc gccgacgggc tgatccacct
      601 gtccgagcta agctggagcc gggtcaacca ccccagcgaa gtgctcaagg tcggccagcc
      661 ggtgcgcgtc tacgtgatga gcgtggacga gcgcgaccgc aagatcgccc tcagcctcaa
      721 gcggacccag cccgagccct ggagccaggt catggagcac tatcaactgg gccaactggt
      781 gcgcggcacc atcacccagt tgaccgactt cggcgcgttc gcgcggctgg aggacggcat
      841 cgagggcctg atccacgtat ccgagctggc cgaaggccgg gtcacgcacc cgcgcaacgt
      901 cgtgcaggaa ggctgggagc tgacgctgcg cgtcatccgc atcgacccgc agcggcggcg
      961 catgggcctg agcctgaagc gtgcgatgga gccgcaggaa ggcgataccc tgcccccgca
     1021 ggagccgctg ccggtgccca ccccgcgcgc cgcgggtgaa ggcgaccagg gtggccgtgg
     1081 tggccggggc ggccagggtg gtcgcggtgg cgccaatcgg ggccggggcc gcgaggaaga
     1141 gcaagccggc ggcggcgagg ccaccgcgca ggcggaagcc ccgccgccgc agccccagcc
     1201 gcagcctgtc gcgccggcgc cggagccgga cctgggcgac acggcgatgg cgcaagccat
     1261 gacggcggcc cgcgcggcgg cggccagtga aaccgccgag gcggaggcgc ccgttaacaa
     1321 tgcagaaccc gccggctctg cctcggatga ggcggaaatg ggcgccggtg agaatgagac
     1381 ccccgccggc gaggccgagg gcgacgacac cggcacggca acttaatagc gagcagtatg
     1441 gggtaggttc cccgccgtcc aggcacatct gcagaacccg tccatatttg tagcctggct
     1501 acccgtcacc ggcgccccgc gaccgtagcg tgtaaccagg ctactgatca tatcgggggg
     1561 gattgcaggg cgccccggca gcggcggatg caaccagaca ggtgtgactt acggtcgggc
     1621 atgcccggcc ggcccaccgc acctgggaat gccccccacc gggggcattt gcgtttatac
     1681 gggagaaaac catggccgaa cgatttgata agtttacgga gcgcgcccgc aaagtcctga
     1741 ctctcgcgca ggaagaagcg caacgcttca accacaacta catcggcacc gagcacatct
     1801 tgctgggcct ggtgcgcgag caggaaggcg tcgcggcgcg ggtgctctcc aacctcggca
     1861 tcgagctacc gaaggtccgc agcgccgtcg agttcatcat cggccgcggc gaccgcatcg
     1921 tcatgggcga gatcggactg actccccgcg cgaagaaggt gatcgagctt gccgtggacg
     1981 aagcccgccg cctgaaccat cactacatcg ggaccgaaca cctgctgctg ggcctggtcc
     2041 gtgagggcga aggcatcgcc gcgggcgtgc tggaaagcct gggcgtctcc ctggagaaag
     2101 tgcgcacgca agtcatccag gtgctcaaca gcagcggcca gcagtacggc cagaagcagt
     2161 cgggccccag caagacgccc tacgtggacg cgctgggcgt cgatctgacc gcgcaggccg
     2221 ccgccggcaa actcgacccg gtgattggcc gcaaa
//
LOCUS       DASUYC010000386         2437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1340342, whole genome shotgun sequence.
ACCESSION   DASUYC010000386 DASUYC010000000
VERSION     DASUYC010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1340342"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            515..1312
                     /locus_tag="VF276_06385"
     CDS             515..1312
                     /locus_tag="VF276_06385"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HEX6606526.1"
                     /translation="MKKWQIATAVAALAVASAGAVGASLAGALPAEGPIIVVPSTVVV
                     ANPNWDPVGPGYNRPATLPVAPALPAATGRVLFSTDFNAPGDPGGTSPLLRDSDLPPA
                     WDVRDGVFQQAGDISGNNRPDEAYYLVGDAAWQDVTVESAILATSGEGAGLVWNVQGD
                     SFYRVQLFPNLPNTAPKARLERVQAGQVTLLAEAPVAAYAGYAPDAWQTVRVENHGAQ
                     RQVWVNGTLLFDVQDTSLTHGQAGLYAWADSGTRFDNLRVQSAAAGN"
     gene            1331..>2437
                     /locus_tag="VF276_06390"
     CDS             1331..>2437
                     /locus_tag="VF276_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606527.1"
                     /translation="MTTRIFPRLARALAGFAVVGAAFAAVPALADPAAPPQAPAAVTF
                     PGPQNASQSGVGASPGDVAGGPTGAASIGWTQGNSTEMDAGSNTGLMAPFLAVQDLDA
                     TNAKLGNVRVAHDSIGRIHAIWYHAYANGSAVIYYGVKNATAGAGGWTVRPIPVTETD
                     STLPYKVDDLAIGPNNRVYVIWGRNNIGVRLTYSDDGVHWAAPEEVPGPFSNTSADFG
                     LGVSTQGVVIVGWFDRYSYDILTRMKINGAWGPLTNISNRGFLTNSYTPRFAVAPDGG
                     LRVIWNEEDPNQRNGDCGARGCRDVWYREWSPATGWDRHLVQLFRSPGETNGYAISVD
                     AAGVSHIVFDDDSHRPARDTTIYYVRGQGTTFTAP"
BASE COUNT          410 a          865 c          789 g          373 t
ORIGIN      
        1 tgtcatgctg aacacagtga agcatctcgt ctcgtttcta tacgagacgc ttcgccgtgc
       61 tcagagtgac aaggcataaa ggccaaactc gcgcctgatt gtaggcgcga gttagcgagc
      121 actcgtcgta ctgaccaccg acccctgacc actcgtcgta ctgaccactg accactgatc
      181 actgaccact cgccatgata acaacttgtg tgccgcgcgg caagatgggt cacgataggg
      241 tcacattgcg gggtctgccg ttgtccgacc ttgcgcgccg cgggggccag tttggtataa
      301 taggacggca gtaagacgct ccggccgcta tccccagggc cgggacgatc taccataatg
      361 cgatacgcgc cccgtcctcg gtcccggctc cacttcccgc agcattccct ttggtctggc
      421 gcccctggac tcccgattct attccattgg cggaatagcg acggtgggcg tgcgtagcgc
      481 atccggtgaa aagcactaca ggaggagatc gtacttgaag aaatggcaaa tagcgactgc
      541 ggtcgcggcg ctggccgtgg catccgccgg cgcggtgggc gcgtccctgg ccggggcgct
      601 cccggcggag gggccgatta tcgtcgtgcc cagcaccgtc gtggtggcga acccgaactg
      661 ggacccggtt ggaccgggct acaatcgccc cgccacgctg cccgtggccc ccgcgctacc
      721 cgcggcgacc gggcgcgtgc tgttcagcac ggatttcaac gcccccggcg atccgggcgg
      781 caccagcccg ctgctacggg actccgacct gccccccgcc tgggacgtac gcgatggcgt
      841 gttccagcag gccggcgaca tcagcggcaa caaccgcccc gacgaagcct attacctggt
      901 cggcgacgcg gcctggcagg atgtgaccgt ggagagcgcc atcctggcga ccagcggcga
      961 gggcgcgggc ctggtctgga acgtccaggg tgacagcttc tatcgcgtcc agttgttccc
     1021 caacctgccc aacaccgcgc ccaaggcgcg gctcgaacgg gtgcaggcgg gccaggtcac
     1081 gctgctcgcc gaggcgcccg tggcggccta cgccggctac gcgcccgacg cctggcaaac
     1141 ggtgcgcgtc gagaaccacg gcgcccagcg gcaggtgtgg gtgaacggca cgctgctgtt
     1201 cgatgtgcag gacacgagcc tgacccacgg ccaggccggg ctgtatgcct gggccgacag
     1261 cggcacccgc ttcgacaatc tgcgggtgca aagcgcggcg gccggtaact aagctagagg
     1321 agattccctg ttgactacgc gtattttccc ccggctggcc cgcgcgctgg ccggattcgc
     1381 cgtcgtgggc gccgcgttcg cggcggtgcc cgcgctggct gatcccgccg cgcccccgca
     1441 ggcgcccgcc gccgtgacct tccccggccc gcagaacgcc tcgcaatcgg gtgtgggcgc
     1501 cagccccggc gacgtggccg gtgggccgac cggcgccgcc agcatcggct ggacccaggg
     1561 taacagcacc gaaatggacg ccggatcgaa caccggcctg atggccccct tcctggcagt
     1621 gcaggatctc gacgccacga acgccaagct cggcaacgtc cgcgtggcgc acgactccat
     1681 cgggcgcatc cacgccatct ggtatcacgc ctatgccaac ggctcggcgg tgatctatta
     1741 cggcgtgaag aacgccacgg cgggcgcggg cggttggacc gtccggccca tccccgtcac
     1801 cgagaccgac agcaccctgc cctacaaggt ggacgacctg gcgatcggcc ccaacaatcg
     1861 cgtctacgtg atctggggcc gcaacaacat cggcgtccgc ctgacctact ccgacgacgg
     1921 cgtgcattgg gccgcgccgg aggaagtccc cggcccgttc agcaacacat ccgccgactt
     1981 cggcctgggc gtgagcacgc agggcgtggt gatcgtcggc tggttcgacc gctatagcta
     2041 cgacattctc acccgcatga agatcaacgg cgcctggggg ccgttgacca acatctcgaa
     2101 tcgcggcttc ctgaccaaca gctacacacc ccgcttcgcg gtggcgccag acggcggcct
     2161 gcgcgtgatc tggaacgagg aagacccgaa ccagcgcaac ggcgactgcg gcgcgcgcgg
     2221 ctgccgcgat gtctggtacc gcgaatggtc ccccgccacc ggctgggatc gccacctggt
     2281 gcagttgttc cgcagccccg gcgagaccaa cggctacgcg atcagcgtgg acgcggcggg
     2341 cgtctcgcac atcgtcttcg acgacgactc gcaccgcccg gcgcgcgaca cgacgatcta
     2401 ctacgtgcgc ggccagggca ccaccttcac cgcgccg
//
LOCUS       DASUYC010000387        10581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_927600,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000387 DASUYC010000000
VERSION     DASUYC010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 10581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10581
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927600"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1313)
                     /locus_tag="VF276_06395"
     CDS             complement(<1..1313)
                     /locus_tag="VF276_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606528.1"
                     /translation="MRLLKSGAGIRLGAAVLAALVVLPGLLWGAGRVAAAPGAQPAGL
                     SMTVQAGFEGHYKLGEWFPVQVTVSNNGAPFDAEVQVDAVGDNDQTLATYARSVALPA
                     PARKQVTLYTYATSFQHTVRVRILQGTTVLLEQRAPIDPLSNGFLLVVVSDAPDQLNY
                     LSGTTLGSTGASGTATVAHLAPEDLPASTAALAGVDALVFAGTDSGKLSPEVRQAIAG
                     WVLQGGTLVVAGGANGPAAAAGLSDLLPVTITGSGPLDSLDALAAYAGGAPPAAAGLI
                     ANQTQLRTDAGATEIVAGAGGPLLARRAIGAGETYALAVDPGAPALRGWDHNTDLWKQ
                     ILAGHRVQQSPAAARRTQSYYSGSNPYIGSSYYLGQLSPFDIPGIELPAVELVGGFLF
                     LYVLVIGPINWLVLRRMHRPDLAWVTIPALIGPVIGPPLGGFITT"
     gene            complement(1774..3144)
                     /locus_tag="VF276_06400"
     CDS             complement(1774..3144)
                     /locus_tag="VF276_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606529.1"
                     /translation="MAHSAPTTQIANGSGDLQPLQRRPHIRHIHDDEEVYGLFHRHYL
                     VFLRGLTAPIVAFLVLVLMGMGISAASPAETTGWVETLLSAGTFVVCGALVAIAAWVV
                     YIFADWRADYMLVTPQRILVNVATPGIHTVLREVPMGKVQNVIIHHASIHEPVKKILQ
                     VARLIIDTAGLGQITFDDISERDADAAKTIIMGLQKNVRAASQPTREQYRHQVMSSII
                     NGTPMPPQPRKTQVRSYPRSGYNFLNRIFPFRPQREGLKVVWHKHWWFLLQAELVPVG
                     IFVLYEITNLIVTLVSGAMGVPTNPVMTLLAFVRPVIFLLLLPIALWQWEDWRNDKYI
                     VDRDRLITISTLPLGLDETVKETEIRRVVDATVRVEGLLPNLLRFGTVVMKTPGEATQ
                     FDFDGVPHPFEVHQEIMTRLEAQREQEQAQWDRDIQDWLRTYVEERRAEPTPPPPPPS
                     DGWTVW"
     gene            3521..4426
                     /locus_tag="VF276_06405"
     CDS             3521..4426
                     /locus_tag="VF276_06405"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX6606530.1"
                     /translation="MIRLLIVVQWIVERLPVSAGNWIADRLGEFAWIVGTRSRCAARS
                     NMRHVLGPAASERAVRRATRAVFRNATRNYYDLLRVGRMSDAALDRAVDFDQESLAMV
                     QKLAEGGKGVLLVSAHWGAFDMVTQVLARRGLSILFLVANFRPAAVAEFMTNLRAQRG
                     SEMVRIDEGLATLKKSMQALKEGRLVGLLPDRNMDRSGVVIPFFGDDTVVATGLAKMA
                     LRARVPVVPGFCYRTGRNRYAVFFTPPIYPPTEGDDTERVTTLTRAVFAVIEKQIARY
                     PEQWTLLQPVWPDAPCPPDPGLAPA"
     gene            4440..5087
                     /locus_tag="VF276_06410"
     CDS             4440..5087
                     /locus_tag="VF276_06410"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX6606531.1"
                     /translation="MANLITLARILLLFITIGLLYLRQPWAAFVSAVLTLIVFVSDAL
                     DGYVARKRGRADMAGAVIDIAGDRVVENIYWVVCAHLNLVPIWAPLVMLVRSFAVDAV
                     RSLALAEGKTAFGDKTMMQSRLGLWLAASRLHRALYGGAKVVTFIYLILQYGLLLWAE
                     QEPGRFNDWGGAWPVIVGVGQALALFSVLYGLARGAVVLYDARDYYLKPPQKGTV"
     gene            5090..5785
                     /locus_tag="VF276_06415"
     CDS             5090..5785
                     /locus_tag="VF276_06415"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX6606532.1"
                     /translation="MESPANPAAGPATPVAIAAVFDVDRTLLPGTTTERLFISYLVRK
                     RVLSLGGLLKTGFYLLRQLPRMSPMEAIRQQRVYLTGQPAARLRALAAQCYEEEIAPR
                     LSQAGRAAVREHREQGHITVLLSGSLDFLLMPLQRDLGADHLISTHLEEKDGRFTGRI
                     DGDWPYGDTKALLINHFAEQHGVDFTASYAYADHHTDEAVLALFGNPVVVNPRTKMQQ
                     IAAGRQWPTREFR"
     gene            5785..6588
                     /locus_tag="VF276_06420"
     CDS             5785..6588
                     /locus_tag="VF276_06420"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX6606533.1"
                     /translation="MHRRTLGLLSGLALGYGLARWMTRPLPPPERVAHRGGAADAPEN
                     TLAAFRNALAAGIPNWELDIQLTADEALVVFHDETLDRTTDGQGPLAGHTLDELKRLD
                     AGAWFGPEWRGERIPTLTEVIDLARTAPNGGARLVIEIKSPHLYPGIEECLLDVLRAE
                     NFMDQVLIMSFDGDSLERVRELAPELPLCYLTGEDSIWPLEPPAHAEILGPKWQLIAL
                     NPLVIVLAHRAGRQVFTWTVNSDLGLRWLRLWGVDGITSDRLDLLQSIR"
     gene            complement(6822..8174)
                     /locus_tag="VF276_06425"
     CDS             complement(6822..8174)
                     /locus_tag="VF276_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606534.1"
                     /translation="MKRTPWTLRALGIATLMGVMLTAPSVGSAQGGSRTFPETGKTVS
                     GQFLDYWNTHGGLAQQGFPISNEMQEKSDLNGQTYTVQYFERAVFEKHPENAPPFDVL
                     LSQLGTYQYKAKYGGNGAPGQVPNNVNGRYFPETKHTIGGSFRTYWEQHGGLAQQGFP
                     ISDEFQEKSDLNGQTYTVQYFERAVFEKHPENQPPFDVLLSQLGTFQYKAKYQGGGGN
                     NPPPTQPPANNGPQNKDANVTPYCGVAGTQFTYFAQGFTPNEALSFWFTAPNGGVVGT
                     SRPAFLAPGDGKFQFPIPTSAQFAQLPGWWAMTWVGDFSKHQSVGYFYVARNAGECAG
                     VPAPQNTTSPPQASCDTSGSKDGKAEPASVQAGHTVRVTAWNFTPNEGLSLWFTAPNG
                     STVGTARPSFRVPGDGGFYLDIPTQPSSDISFSPGRWAITFEGASSHHQSVIFFCVTP
                     "
     gene            complement(8922..10358)
                     /gene="rimO"
                     /locus_tag="VF276_06430"
     CDS             complement(8922..10358)
                     /gene="rimO"
                     /locus_tag="VF276_06430"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEX6606535.1"
                     /translation="MDVKKAPSFNIVTLGCEKNSVDSEGMGQVLAESGYTDAARPEDA
                     DVLIVNTCGFLQASTGESVAALQDLAAHKRPDQLLVAAGCATQRYGAAIADQVPGVDG
                     LLSTRQWPQIARLVEHLRTQQANGELPAQQTGADPVAAIQAHAASIEQALLAPVQPTA
                     ELKRFKRKASGASAYVKISEGCDHTCAFCIIPDIRGKHVSKPIDEIAAEVRDLSEQGV
                     REAILIAQDSTYYGTDLRMRDGLAQLLEAIARDAPAMEWVRLMYAYPTQITPRLIETM
                     AALSQVCKYLDMPLQHGHPETLRRMRRPNNIDRVRRLIAGLRAAMPEIALRTTFIVGF
                     PGETDAEFQALLDFIAEIRFDHVGMFMYSAEEGTRAATLPDQIPERVKQRRYKQAMLL
                     QQGISAEQNQRWVGRTLDVLIEGTGTVDDGRGHKRPILAGRYYRQAPEVDGMVFVKGQ
                     PGVEVGQTVPVRITQSTEYDLWGKVVRA"
     gene            complement(10456..>10581)
                     /locus_tag="VF276_06435"
     CDS             complement(10456..>10581)
                     /locus_tag="VF276_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF520 family protein"
                     /protein_id="HEX6606536.1"
                     /translation="QIQGDALRITAKSKDELQAVIKFLREHEADYPIALQFTNYR"
BASE COUNT         1690 a         3562 c         3490 g         1839 t
ORIGIN      
        1 taggtggtga tgaagccgcc gagcggcggt ccgatcaccg ggcctatcag cgcggggatc
       61 gtcacccagg ccaggtccgg gcggtgcatc cggcgcagca ccagccagtt gatcggcccg
      121 atcaccagca catacaggaa caggaagccg ccgaccagct ccacggccgg caattcgatg
      181 ccggggatgt cgaacgggct aagctggccc aggtaatacg acgacccgat gtagggattc
      241 gagccgctgt aatagctctg ggtgcgccgg gccgccgccg gggactgctg cacccggtgc
      301 ccggccagga tctgcttcca caggtcggtg ttgtggtccc agccgcgcag cgccggggcg
      361 cccggatcga cggccagggc gtaggtctcg cccgcgccga tagcccggcg cgccagcagc
      421 gggccgcccg ccccggccac gatctcggtc gcgcccgcat cggtgcgcaa ctgtgtctgg
      481 ttggcgatca gcccggcggc ggcgggcggc gccccacccg cataggcggc cagcgcgtcc
      541 aggctatcca gcggcccgct gccggtgatc gtgaccggca agaggtcgct caggcctgct
      601 gccgccgccg ggccgttggc gcccccggcc accaccagcg tgccgccttg cagcacccac
      661 ccggcaatcg cctggcgcac ttcggggctg agtttgccgc tgtcggtgcc ggcgaagacg
      721 agcgcgtcga cgccggccag cgcggcggtg ctcgccggta gatcctccgg cgccaggtgc
      781 gcgacggtgg ccgtgcccga cgcgccggtg ctgcccagcg tggtgcccga caggtaattt
      841 agctggtcgg gcgcgtcgct gaccacgacc agcaggaagc cgttgcttaa gggatcgatc
      901 ggcgcccgct gctccagcag caccgtggtg ccctgcaaaa tacgcacacg cacggtgtgc
      961 tggaacgagg tggcataggt gtacagcgtg acctgtttgc gtgcgggcgc gggcagggcc
     1021 accgaccggg cataggtcgc cagcgtctgg tcgttgtcgc ccacggcgtc cacctggact
     1081 tcggcgtcga acggcgcgcc gttgttgctc accgtgacct gcacggggaa ccactcgccc
     1141 agcttgtaat ggccctcgaa cccggcctgc acggtcatgc tcaggcccgc gggttgggcg
     1201 cccggcgcgg ccgccacccg gcccgccccc cacagcagcc ccggcagcac gacaagcgcc
     1261 gccagcacgg cggcgcccag ccggattcct gcccctgatt tcaacaatcg caagtcgctc
     1321 tcctgtatcg acgcccagat ttcgcagatt tcggcgaatt ttgattgtag gggccggttt
     1381 caaaccggcc caacccgtta ctggatagac gcctggttgt ccccgcactg cttctgatgg
     1441 gcggcagcca gtttgaaacc gcggccccta caccaggcca gccgggccgg tttcaaaccg
     1501 ggccctacac cgggcaacca ggccagttgg aaaccggccc ctacaccggg caaccaggcc
     1561 ggtttgaaac cggcccctac acggcaaaat cacgcgaaat cccggtggcg gactggtaga
     1621 acccacatta gaaagacgta atcgcgtccc gcaaagttgc aggccccgct acgggtggct
     1681 gccccatcag gcccggccaa tactgaggtc atccagcccg tgcaggcggg ctttgcagag
     1741 ctagccggcc tcttgagggg ccggaccgcg ccgctaccag accgtccagc cgtcggaggg
     1801 cggcgggggg ggcggagtcg gctcggcccg ccgttcttcg acgtaggtgc gcaaccagtc
     1861 ctggatgtcg cggtcccact gcgcctgctc ttgctcgcgt tgcgcttcga gccgggtcat
     1921 gatttcctga tgcacctcga aggggtgggg gacgccgtca aaatcaaact gggtggcctc
     1981 gcccggcgtc ttcatcacca ccgtgccgaa gcgcagcagg ttgggcagca gcccctcgac
     2041 ccgcaccgtg gcgtccacca cccggcggat ctccgtctct ttcaccgttt catcgaggcc
     2101 cagcggcaag gtgctaatgg tgatcaagcg atcacgatcg acgatatact tatcgttgcg
     2161 ccagtcctcc cactgccaga gggcaatggg cagcaacagc aggaagataa ccgggcgcac
     2221 aaacgccagc agcgtcatca ccgggttggt gggcacgccc attgctccgc tgaccagcgt
     2281 gacgatcaga ttggtgatct catacaggac aaagatgccc accggcacca gctccgcttg
     2341 cagcaggaac caccagtgct tgtgccagac caccttgaga ccctcgcgct gcggccggaa
     2401 ggggaagatt cggttgagga agttgtagcc gctgcgcggg taggaccgca cctgggtttt
     2461 gcgcggctgc ggcggcatgg gcgtgccgtt aatgatgctg gacatcacct ggtgccgata
     2521 ctgctcgcgg gtcggctggc tggcggcgcg cacgtttttc tgcaggccca tgatgatcgt
     2581 cttggccgca tcggcgtcgc gctcgctgat atcgtcgaac gtgatctggc cgagtcccgc
     2641 cgtgtcgatt atcaatctag cgacctggag gatcttcttg accggttcgt ggatcgaggc
     2701 gtggtggatg atgacgtttt gcactttgcc catcggcacc tcgcgcagca ccgtatggat
     2761 gccgggcgtg gcgacgttga ccaggatgcg ttgcggcgtg accaacatgt agtcggctcg
     2821 ccagtcggcg aagatataga ccacccaggc ggcaatcgcc accagggcgc cacagaccac
     2881 gaaagtgccc gccgagagca gcgtttccac ccagccggtc gtctccgccg ggctggccgc
     2941 gctgatcccc atgcccatga gcaccagcac caggaaagcc acgatcggcg cggttagccc
     3001 gcgcaggaag actagatagt ggcggtgaaa cagcccgtag acttcttcgt cgtcgtggat
     3061 atggcggatg tggggccgcc gctgcagggg ctgtagatcg cccgatccat tggcgatttg
     3121 tgttgtcggt gcggagtggg ccatgccctc acccttcctc gccagccgcc tgccgccggc
     3181 ctcaccgggc ccggccccgg cccgccgggg gtcacaccgt gcctgcgccg tctcgcggca
     3241 accgggatcg gcaactgtag cgtaatctta ctacacataa cataacagcg tcaaccggac
     3301 gggtgattag ccttcagcta tcagccatca ggagacgagt ggtcagtaag ctgctacgtg
     3361 tggttacgcg gaatttcgtc catgcgcagc acatcatctc gccgagtctg atcctactcc
     3421 gcactcgtct cctgacggct gatggctgaa ggctgatcac ttgccggcac tcgcacgccg
     3481 ggagcgcgtt tgctataata gccgcggaga aaggagcgac atgatacggc tgctcatcgt
     3541 ggtacaatgg attgtggaac ggctgccggt gtccgccggc aactggatcg ccgaccggct
     3601 gggcgagttc gcgtggatcg tgggcacgcg gtcacgctgt gcggcgcgca gcaatatgcg
     3661 ccatgtgctg gggccggcgg cctccgaacg ggcggtgcgc cgggcgacgc gcgccgtgtt
     3721 ccgcaacgcg acccgcaact attatgatct gttacgggtg ggccgcatga gcgatgccgc
     3781 gctcgaccgg gcggtggact tcgatcagga aagcctggcg atggtgcaaa agctggcgga
     3841 gggcggcaag ggcgtcttgc tggtgtcggc ccattggggc gccttcgata tggtgacgca
     3901 ggtgctggcc cggcgcgggc tgtcgatcct gttcctggtc gccaatttcc gccctgccgc
     3961 cgtcgccgag tttatgacca acttgcgcgc ccagcgcgga tccgaaatgg tgcgcatcga
     4021 tgagggcctc gccacgctga aaaagtcaat gcaagcgctc aaggaaggcc ggctcgtggg
     4081 cctgctgccc gaccgcaaca tggatcgctc gggggtggtg atcccttttt tcggcgacga
     4141 cacggtggtc gccacggggc tggcgaagat ggccctgcgc gcgcgcgtcc cagtggtgcc
     4201 cggcttctgc taccgcaccg ggcgcaaccg ctatgccgtc ttcttcaccc cgccgattta
     4261 cccgcccacc gagggcgacg acaccgaacg tgtgacgacc ctgacgcgcg cggtttttgc
     4321 ggtcatcgag aagcagatcg cgcgctaccc ggagcaatgg acgctgctgc agccggtctg
     4381 gcccgacgcg ccctgccccc ccgaccccgg tctcgcgccg gcatagaggc tcatgttgta
     4441 tggctaacct gattaccctc gcgcgcatcc tgctcctgtt catcaccatc ggcctgctct
     4501 acctgcgcca gccctgggcc gccttcgtct ccgcggtgct caccctgatc gttttcgtca
     4561 gcgatgcgct cgacggctac gtggcgcgca agcggggccg cgccgatatg gccggggccg
     4621 tgatcgacat cgccggcgac cgggtggtcg agaacatcta ctgggtcgtc tgcgcccacc
     4681 tgaacctcgt gccgatctgg gcgcccctgg tgatgctggt ccggtccttc gcggtggacg
     4741 ccgtgcgcag cctggccctg gccgagggca aaacggcgtt cggcgacaag acgatgatgc
     4801 aatcgcggct gggcctctgg ctggctgcgt cgcgcctgca ccgcgcgctc tacggcgggg
     4861 ccaaggtcgt cactttcatt tacctgattt tgcagtatgg cttgctgctc tgggccgaac
     4921 aggaaccggg ccgtttcaac gactggggcg gcgcctggcc ggtcatcgtc ggcgtgggcc
     4981 aggcgctggc gttgttcagc gtgctctatg gcctggcccg cggcgccgtg gtgctgtatg
     5041 atgcgcggga ctactatctc aagccgcctc agaaaggaac cgtctaagca tggaatctcc
     5101 ggccaatccc gccgcgggac cggccacgcc cgtcgctatc gccgccgtct tcgatgtcga
     5161 ccgcaccctg ctgcccggca cgaccaccga gcggctgttt atcagctacc tggtgcgcaa
     5221 gcgggtgctg agcctgggcg ggctgctgaa aaccggcttc tacctgctgc gccagttgcc
     5281 gcgcatgagc cccatggaag cgatccgcca gcagcgcgtc tacctgaccg ggcagcccgc
     5341 cgcgcgcctg cgcgccctgg ccgcgcagtg ctatgaggaa gagatcgccc cgcggctgtc
     5401 ccaggcgggc cgcgctgccg tgcgcgagca ccgcgagcag ggccatatca cggtgctgct
     5461 ctccggctcg ctcgatttct tgctgatgcc cttgcagcgc gatctcggcg ccgaccatct
     5521 gatctccacc cacctggagg aaaaagacgg gcgctttacc gggcgcatcg acggcgactg
     5581 gccctacggc gacaccaagg cgctgctgat caaccatttc gccgagcagc atggcgtcga
     5641 tttcaccgcc agctacgcct atgccgacca ccataccgac gaggccgtgc tggccctctt
     5701 cggcaaccct gtcgtcgtca atccgcgcac caagatgcag cagattgccg ccggacggca
     5761 gtggcccaca cgggagttcc gctaatgcac cgccgtaccc ttggcctgtt gagtggcctc
     5821 gcgctgggct atggcctggc ccgctggatg actcgcccgc tgccgccgcc cgagcgcgtg
     5881 gcccaccgcg gcggcgccgc cgatgccccg gagaataccc tggccgcctt ccgcaacgcc
     5941 ctggccgccg gcatccccaa ctgggaactc gatatccaac tcaccgccga cgaggccctc
     6001 gtcgtcttcc acgacgagac gctcgaccgc accaccgacg gccagggacc gctggccggg
     6061 cacacgctgg atgagttgaa gcgcctggat gccggggcct ggtttggtcc ggagtggcgc
     6121 ggcgagcgca ttcccacgct gaccgaggtg atcgacctgg cccgcacggc gcccaacggc
     6181 ggcgcgcgcc tggtcatcga gatcaaaagc ccgcaccttt accccggcat cgaagaatgc
     6241 ctgctcgacg tgctgcgcgc cgagaacttc atggaccagg tgctgatcat gtccttcgac
     6301 ggcgactccc tggagcgcgt gcgcgagctg gcccccgagc taccgctctg ctacctcacc
     6361 ggcgaggact cgatctggcc gctggaaccg cccgcccacg ccgagatcct tggcccgaag
     6421 tggcagttga tcgctctgaa cccgctggtc atcgtgctgg cccaccgcgc cgggcgccag
     6481 gtcttcacct ggacggttaa cagcgacctg ggcctgcgct ggctgcgcct ctggggcgtg
     6541 gacggcatca ccagcgaccg cctcgatctg ctgcaaagca tccgctgatc ccggcgcccc
     6601 ctatatcagt ccagtagttc accgcgaaga cgcgaagaac gcgaagacta tttatgtagg
     6661 tcttattctt cgcgtccttc gcgtcttcgc ggtgcctaat agggcagtca gccgcgacgg
     6721 cgggcgccgg gaaagcgcaa tcgcgccgct tcccggagga ggcgacgcga ttggagcgcc
     6781 ggggcgcgga gagggcgccc cggcggggta gcgcgcggcc cttacggggt cacgcagaag
     6841 aagatgaccg actggtggtg gctgctggcg ccctcgaagg tgatcgccca gcggcccggc
     6901 gagaagctga tgtcgctgct cggttgcgtc gggatgtcca gatagaaccc gccgtcaccc
     6961 ggtacgcgga acgagggccg cgcggtgccc acggtgctgc cgttgggggc ggtgaaccac
     7021 aggctcaacc cctcgttcgg cgtgaagttc cacgcggtca cgcggacggt gtgcccggcc
     7081 tgcaccgacg ccggctcggc cttgccgtcc ttgctgccgc tggtgtcgca actggcctgc
     7141 ggcggcgagg tggtgttctg cggcgcgggc acgcccgcgc actcacccgc gttgcgcgcc
     7201 acgtagaagt agccgaccga ctggtgcttg ctgaagtcgc cgacccaggt catcgcccac
     7261 caccccggca gttgcgcgaa ctgcgcgctc gtcgggatgg ggaactggaa cttgccgtcg
     7321 ccgggcgcca ggaaggccgg gcgggaggta cccaccacgc cgccgttcgg cgcggtgaac
     7381 cagaaggaca gggcttcgtt cggggtgaaa ccctgggcga agtaggtgaa ctgcgtcccc
     7441 gcgaccccgc aatacggcgt cacattggcg tccttgttct gcggcccgtt gttggccggg
     7501 ggctgcgtcg gcggcggatt gttgccgccc ccgccctggt acttggcctt gtactggaac
     7561 gtgccgagct ggctcagcag cacgtcaaac ggcggctggt tctcggggtg cttctcgaag
     7621 acggcgcgct cgaaatactg caccgtgtag gtctgcccgt tcaggtccga cttctcctgg
     7681 aactcgtcgc tgatcgggaa gccctgctgg gccaggccac cgtgctgctc ccagtacgtg
     7741 cggaagctgc cgccgatggt gtgcttggtt tcggggaagt agcggccgtt cacgttgttc
     7801 ggcacctggc ccggcgcgcc gttgccgcca tacttggcct tgtactgata ggtgccgagc
     7861 tggctcagca gcacgtcaaa cggcggcgcg ttttcggggt gcttctcgaa gacggcgcgc
     7921 tcgaaatact gcaccgtgta ggtctgcccg ttcaggtccg acttctcctg catctcattg
     7981 gagatcggga agccctgctg ggccagcccg ccatgcgtgt tccaatagtc gaggaactgg
     8041 ccgctgaccg tcttgccggt ctcggggaac gtgcgcgatc cgccctgtgc ggaacccacg
     8101 ctcggcgcgg tcagcatcac gcccatcagg gtcgcgatac ccagcgcgcg aagcgtccag
     8161 ggtgtgcgtt tcatgagaat tcctttcaaa caatacgaat ggggatagca ggcgcgctgc
     8221 acgcttggta gaaatgccag ggccacagca gcatacgcgc cgtggacccg ccggaccctt
     8281 ggatggctct cgtgccgcgc ctctcccgcc aacacgatgg agtggattct gtaccggcgt
     8341 gcccaggttc ctgagaatct gcccggtgaa ccagatcacc ggattccctt ccgatccttg
     8401 cgcttccggc ctctttcgga taactcgtca cgcctgtgcg gcgtccacca gtctataatg
     8461 cgcttggtgg aaaggagtta ccgaatcagg acttggaaaa cgggatcgaa ttttggggcg
     8521 aagccagatg gcaaaacggc cgtcacgtct gagggggcgg tgggccaact gatgcgcacg
     8581 cggccccagg aagcttcttt ttcgtagaag ccgcgccatt gtagcaccag ccccgcgccc
     8641 ccgtcaaccg gcagcggcgc cggccaaggc ccgccgggct gtccgcataa gcgaacggta
     8701 agctgcgcgg taaggctgga tcgcggctac gcacgatcca cgcaatacac gggtgctgta
     8761 aaggtctaat ggtccctacc ttttccttac cattggacgc aaagaaaagg ccccccagcc
     8821 caccagcaac ccaaaccgca gaaaaaacca ttctggaagc agacctgtgc aagcggaaaa
     8881 aaaggttccc ccttcccgcg ccgggaaggg gggcaggggg gttaggcccg caccaccttg
     8941 ccccagaggt catactcggt ggactgggtg atacgcacgg gcaccgtctg ccccacctcg
     9001 acgccgggct ggcccttcac gaagaccatg ccgtccacct ccggcgcctg gcggtaatag
     9061 cgcccggcca ggatggggcg tttgtgcccg cgcccgtcgt ccaccgtgcc ggtgccctcg
     9121 atcagcacgt cgagcgtgcg gcccacccag cgctggttct gctccgccga gatgccctgt
     9181 tgcagcagca tagcctgttt gtagcggcgc tgcttcaccc gctccgggat ctggtcgggc
     9241 agggtggccg cgcgggtgcc ctcttccgcc gagtacatga acatgccgac gtggtcgaac
     9301 cggatctccg caatgaaatc gagcagcgcc tggaactcgg cgtcggtctc gccggggaag
     9361 ccgacgatga aggtggtgcg cagggcaatc tcgggcatag cggcgcgcag gccggcgatc
     9421 aggcggcgca cgcggtcgat gttgttcggg cggcgcatgc ggcgcaacgt ctcggggtgg
     9481 ccgtgctgca acggcatatc gaggtacttg cagacctggg acagggcagc catggtctcg
     9541 atcagccgcg gcgtgatctg cgtcgggtag gcgtacatca ggcgcaccca ctccatcgcc
     9601 ggcgcgtcgc gggcgatggc ctccagcagt tgggccaggc cgtcgcgcat gcgcagatcg
     9661 gtgccgtagt acgtgctgtc ctgggcgatc aggatcgcct cgcgcacgcc ttgctcgctc
     9721 aggtcgcgca cctcggcggc gatctcgtcg atgggcttgc tgacgtgctt gccgcggatg
     9781 tcggggatga tgcagaaggc gcaggtgtgg tcgcagcctt cggagatctt cacataggca
     9841 ctggcgccgc tggccttgcg tttgaagcgc ttgagttccg ccgtgggctg cacgggggcc
     9901 agcagggcct gctcgatgct cgccgcgtgg gcctggatgg ccgccaccgg gtcggcgccc
     9961 gtctgctggg ccggtagctc gccgttggcc tgctgcgtgc gcagatgctc caccaggcgg
    10021 gcgatctgcg gccactggcg cgtgctcagc aggccgtcca cccccggcac ctggtcggcg
    10081 atggccgcac cgtagcgctg cgtggcgcac ccggcggcca ccagcaactg gtcgggccgc
    10141 ttgtgcgccg ccaggtcctg caacgccgcc accgactcgc cggtggacgc ctggaggaag
    10201 ccgcaggtgt tgacgatgag cacgtcggca tcctcgggcc gcgcggcatc cgtatagccg
    10261 gactcggcca gcacctggcc catgccctcg ctgtccaccg aattcttctc gcagcccagc
    10321 gtgacgatat tgaagctggg cgcctttttt acatccatga gcctgccttg ctagtgcctg
    10381 tcggatattg atgctggtgg gcggcgggcc ggtttcaaac cggcccctac accgcaaaat
    10441 cgcgccggcc gattgttagc gataattggt gaactgcagc gcgatcgggt aatcggcttc
    10501 gtgctcgcgc aggaacttga tcaccgcctg caactcgtcc ttgctcttgg ccgtgatgcg
    10561 cagggcatcg ccctggatct g
//
LOCUS       DASUYC010000388         4052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_828844,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000388 DASUYC010000000
VERSION     DASUYC010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828844"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VF276_06440"
     CDS             complement(<1..530)
                     /locus_tag="VF276_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX6606537.1"
                     /translation="MRHSAAHLMAGAVLRMFPEARFGIGPAIEDGFYYDFELPRTLTP
                     DDLAEIEAGMAADQAANYPYEYAVVSRDEALDLFKDQPYKVELIENLPEDATISTYQH
                     GHFRDLCRGPHVPSTGAVGAFKLLNVAGAYWRGDEHRPMLQRIYGTAFPSQEALDQYL
                     WRRAEAEKRDHRKLGK"
     gene            1347..2978
                     /locus_tag="VF276_06445"
     CDS             1347..2978
                     /locus_tag="VF276_06445"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6606538.1"
                     /translation="MLHNSAKGPLILVADDSRDVRRNLQKLLEYHGCRVCTADDGQGA
                     LEAIRRHPPDLVLLDVNMPRLDGLATVRLLKADRDVGHIPVILLTALSGAESLLHGLD
                     AGADEFLSKPVDEPELLARVRSLLRIKQQHDRLRDYQDHLAQLLRISEQLLTRLEPHQ
                     LLSAIVELATQHLGFRRCIVTVLDPQTGILKVGAVAGVSDRKTVTALTRTTYRWAAVQ
                     ALLRPEYRISESYLIPDETPVRNRRNGHKPATPVGNEWRPQDNLLVPLRATGGTLYGF
                     LSFDTPDDGLRPGVEQIQLLELFAHQAAAAMQNAHLYADLHQQYARYVGPVVAAQLTA
                     RTAAGPPAPPVEQDLVVLFSDLRGFTALSEGLSPQELVNEVLNPYFSHMTDVIVAYDG
                     MVDKFLGDGIMALFGVPQRRGDEAARAITAALAMQEAFQPLQAEWHARLGRDIGMGIG
                     LASGKAIVGSIGSTHRTDYTAIGSVVNLASRVTDLTPTGQVWATAELKLLAETYLLAH
                     PDAGPRYPITFRPLMPTTIKGTSGMQSLYVCLVNR"
     gene            3148..>4052
                     /locus_tag="VF276_06450"
     CDS             3148..>4052
                     /locus_tag="VF276_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEX6606539.1"
                     /translation="METVVQGPGAGKDAVPDIYLDHAATTPVHPDVVQAMLPFFTARA
                     GNPSSVHHRGRAANQGLESARRAVAGVLGCRPREIVFTGGGSEADNLALKGAAYAHRM
                     AGHATSHIISIPIEHHAIGHTLDFLETQGFAVTQVPVDSTGRVSVADIERAIRPDTCL
                     ITVMYANNEIGTIQPIAEIGALARRHGILFHTDAVQAGGALPMRVADLNVDLLSLSAH
                     KFYGPKGVGVLYVRTGTKIFPILHGGGQEHSLRAGTENVPGIVGLAAALTRAHATREQ
                     EVAHVTALRDRLIAGIQARIPDAHL"
BASE COUNT          666 a         1498 c         1225 g          663 t
ORIGIN      
        1 tccttgccca gcttgcggtg gtcacgcttc tcggcctcgg cgcgccgcca caggtactgg
       61 tccagcgctt cctgggacgg gaaagcagta ccgtagatgc gctgcaacat gggccggtgc
      121 tcgtcgccgc gccagtaggc ccccgccacg ttgagcagct tgaacgcccc gacggcgccc
      181 gtgctcggca cgtgcggccc gcggcacagg tcgcggaagt gcccatgctg gtacgtcgag
      241 atcgtggcgt cctccggcag gttctcgatc agttcgacct tgtagggctg gtctttgaac
      301 aggtccaaag cctcgtcccg gctcaccacg gcgtactcat aggggtagtt ggccgcctgg
      361 tccgccgcca tccccgcctc gatctccgcc aggtcgtccg gcgtcagcgt gcgcggtaac
      421 tcgaagtcat agtagaagcc atcctcgatg gccggcccga tgccgaatcg ggcctccggg
      481 aacatcctca gcaccgcccc ggccatcagg tgcgccgccg aatggcgcat gcgcgccagc
      541 cgcgccgcct cggactccgg cccctccgcc gccaccgggt gtggtgcgta atccagttcc
      601 agatcgctca tttgtcgtct ccttatagct atcagccgtc agccatcagt ggtcagtggt
      661 cagtggtcag tggtcagtaa ttagcagttc gtcttgccca tccccgctca ccaacgactg
      721 acccctcgtc ctcacttcac actcgtccga ctcaacactc gttcttctga tcactgacca
      781 ccgaccactg actactcgta ctcagcactc agcactcagg actaaaaaaa gcctcgcgtc
      841 ccaacacggg acgcgaggct caaactcacg tggttccacc cgattgcacg ccgccgggca
      901 ggccggggcg cctcatcgtg gccggtaacg gggccatccg tcctgcgact actcgcggtc
      961 aaaaccgctt ttgccgggcc gttcccaggg ggttttcgtg ggggcgtgga ccgacccggc
     1021 ttgcagccgg tgaccgggtc tctctgcggg gcgcccgccc gtactcgtcc tgttcgcgac
     1081 gtttgcgcta tcgggttgta gtccaattat accgccccat cccgccccat tgcaagcgcc
     1141 gaaagaccga acgcggggac caatcggcct aattgcggag gcgctctttc cttgaaagcc
     1201 ggcgtatccc caggggccgg gccaaggtat gatataatga gccgcgacaa cggcccgcgg
     1261 cctcgtcccc agcgagtgcg gcccggcccg cgtcacggga cccgcaggct acgggaccct
     1321 gctatggatg tcatgaccgg agtcatatgc tgcataactc cgccaaagga ccactgattc
     1381 tggttgcaga cgatagccgg gatgtacggc gcaatctgca aaagttgctg gaataccacg
     1441 gctgccgggt ctgcaccgcc gacgacggcc agggggcgtt agaggcgatt cgtcgccacc
     1501 cgcccgacct ggtgctgctc gacgtgaata tgccccggct cgatggcctc gccacggtgc
     1561 gcttgctcaa agccgaccgt gacgtgggcc acatcccggt gatcttgctc accgccctca
     1621 gcggcgccga atccctgctg cacggtctcg atgccggcgc cgacgagttc ctctccaagc
     1681 cggtggacga gcccgaactg ctggcgcgcg tccgctcgct cctgcgcatc aagcagcagc
     1741 acgaccgcct gcgcgactac caggatcatc tcgcgcagtt gttgcgcatc agcgagcagt
     1801 tgctgacccg gctggagccg caccagctcc tgagcgccat cgtcgaactc gcgacccagc
     1861 acctcggctt ccgccgctgc atcgtgaccg tgctggaccc gcagaccggc atcttgaagg
     1921 tgggcgcggt ggccggagtg agcgatcgca aaaccgtcac cgctcttaca cgcaccacct
     1981 accgctgggc ggccgtccag gccctgctcc gccccgagta ccgcatcagc gagtcctacc
     2041 tcatccccga cgaaacgccg gtgcgcaacc gccgcaacgg ccacaagccc gcgaccccgg
     2101 tgggcaacga gtggcgcccg caggacaact tgctcgtgcc cttgcgcgcc accggcggca
     2161 cgctctacgg cttcctctcg ttcgatacgc ccgacgacgg cctgcgcccc ggcgtcgagc
     2221 agatccaact gctggagttg ttcgcccacc aggccgccgc cgccatgcaa aacgcccacc
     2281 tctacgccga cctgcaccag cagtacgctc gctatgtcgg cccggtggtc gccgcccaac
     2341 tcaccgcgcg caccgccgcg gggccccccg cgcccccggt ggagcaggac ctggtggtgc
     2401 tcttctccga tctgcgcggc ttcaccgccc tgagcgaagg gctgagtccg caggagctgg
     2461 tcaacgaggt gctcaacccc tatttctcgc acatgaccga cgtgatcgtg gcctacgacg
     2521 gcatggtcga taagttcctg ggcgacggca tcatggccct cttcggcgtg ccccagcgcc
     2581 gcggcgacga ggcggcgcgc gccatcaccg ccgccctggc gatgcaggag gcttttcagc
     2641 ccctgcaagc cgagtggcac gcccgcctgg gccgcgacat cggcatgggc atcgggctgg
     2701 cgtcgggcaa ggccatcgtc ggcagcatcg gctcgaccca ccgcaccgac tacacggcca
     2761 tcggcagcgt cgtcaacctc gccagccgcg tcaccgacct caccccgacc ggccaggtct
     2821 gggccaccgc cgaactcaag ctgctggccg aaacctatct gctggcccac cccgacgccg
     2881 gcccgcgcta tcccatcacc ttccgcccgc tcatgcccac cactatcaag ggcaccagcg
     2941 gcatgcaatc cctctacgtc tgcctcgtca accggtaacg cgggcggcaa gcgcctattg
     3001 cctacgcagc acctcggact cgccaactgc ccgccgccgg cacgtgtggt ataatacggc
     3061 gaatcggtcg gatatccgga agcttgcggt ttatgctgcc atggtgtaga tcgcgctatc
     3121 ttaatcaaag gagcttagat agcatttatg gagacggtag tacaggggcc gggggccggc
     3181 aaggacgccg tgcccgatat ttatctcgat catgcggcga ccacgccggt ccaccccgac
     3241 gtcgtgcagg ccatgctgcc cttctttacc gcgcgggcgg gcaatccgtc gagcgtgcac
     3301 catcgcgggc gggccgccaa ccaggggctg gagtcggcgc ggcgcgccgt ggccggggtg
     3361 ctgggctgcc gcccgcgcga gatcgtgttc accggcggtg gcagcgaggc cgataacctg
     3421 gccctgaagg gcgccgccta cgcccaccgc atggccggcc acgccacctc gcacatcatc
     3481 tcgatcccca tcgagcacca cgccatcggc cacacgcttg acttcctgga gacgcaggga
     3541 ttcgccgtca cccaggtgcc cgtggatagc accgggcgcg tcagcgtggc cgacatcgag
     3601 cgcgccatcc gccccgacac ctgcctgatc accgtcatgt acgccaacaa cgagatcggc
     3661 acgatccagc cgattgccga gatcggtgcg ctggcccggc ggcacggcat cctgttccat
     3721 accgacgcgg tccaggcggg cggcgcgctg cccatgcgcg tggccgacct caatgtagac
     3781 ctgctctcgc tctcggcgca caagttctac ggccccaagg gcgtcggcgt gctctacgtg
     3841 cggaccggga cgaagatctt ccccattctg cacggggggg ggcaggagca cagcctgcgc
     3901 gcgggcaccg agaacgtacc gggcatcgtc ggcctggccg ccgcgctcac ccgcgcccac
     3961 gccacgcgcg agcaagaggt ggcccatgtc acggccctgc gcgaccggct gatcgccggc
     4021 atccaggcgc gcatccccga cgcccatctg ca
//
LOCUS       DASUYC010000389         2534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1879851, whole genome shotgun sequence.
ACCESSION   DASUYC010000389 DASUYC010000000
VERSION     DASUYC010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1879851"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..1358
                     /locus_tag="VF276_06455"
     CDS             153..1358
                     /locus_tag="VF276_06455"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6606540.1"
                     /translation="MATEYDVVVAGAGPAGAVTAGLLARAGLRVALLDKAAFPRDKAC
                     GEYTSPQTATVLARIGALPYVERARPRRLPAMRVYARNGAWFAMDYAAQGGGAVLATP
                     RRRLDAALVEYATAGGAELQEHTRVVEVIREDGSVRGVRVRRGAGAEQAIHAALVVGA
                     DGSHSAVARALGLNRTPRWPRRLGMVAHYAGVRGCDAWGEMHAAAHGYCGLAPLADGL
                     VNVGIVVRMPGPAAPRLSSEARFEAALGAFPWLADRMAAARRVTPVRGVGPLVVDAVQ
                     ASGAGFVLVGDAAGFFDPFTGEGVYKAVRGAELAAPVILRALAVGDLSARALAPYGAA
                     RRREFLAKDLVCRIVQGFVQVPAGLDYVAPRLAGRPHHLAIMAGVLGDFTDARQALSP
                     AYLLGLLRP"
     gene            1504..2052
                     /locus_tag="VF276_06460"
     CDS             1504..2052
                     /locus_tag="VF276_06460"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX6606541.1"
                     /translation="MTTQAKTTTADDLLNLPDDGFRYELVEGDLHKMPPPGRRHARII
                     AKLIAALEQYLSEQRLGEVYADFGYLLAENPDTVLAPDVSFLRQERLAEADPDRPYWV
                     GAPDLAVEVVSPGDRYSEVNEKVGRWLAAGTLLVWVIDPRRQQIAVHQAPTTVTNLTL
                     ADTLNGGDVLPGWTLPVREVFA"
     gene            2115..>2534
                     /locus_tag="VF276_06465"
     CDS             2115..>2534
                     /locus_tag="VF276_06465"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX6606542.1"
                     /translation="MCYDDNARPPVPPGPGGAAEGEDLVLTAADGTRFAAYAARPANP
                     TGAQILIYPDVRGLHQFYKDLALRFAETGVRAVAIDYFGRTAGLTSRAEPFDYMPHVQ
                     QMELPHVFADTRAALDYLRAGAGPGNRTFLIGFCRGGS"
BASE COUNT          351 a          838 c          930 g          415 t
ORIGIN      
        1 gagtgattct caccgcgaag gcgcgaagga cgcgaaggat tcttagggtt tcgggaatga
       61 ggactatcgc cctacataag gtaaaccctc aaagctcttc gcgtccttcg ttccttcgcg
      121 gtgcaataat cggctagtag ccagggtgga gcatggcgac ggagtatgac gtggtcgtgg
      181 cgggggcggg gccggcgggc gcggtgacgg cggggttgct ggcgcgtgcc gggctgcgcg
      241 tggccctgct cgacaaagcg gctttcccgc gcgacaaggc gtgcggcgag tataccagcc
      301 cgcagacggc gacggtgctg gcgcgcatcg gcgcgctgcc ctatgtcgag cgcgcgcggc
      361 cccgccgtct gcccgccatg cgcgtctacg cccgcaacgg cgcctggttc gcgatggact
      421 acgcggcgca gggcggtggc gcggttctcg ccacgccgcg gcgccgcctg gatgccgcgc
      481 tggtcgaata cgccacggct gggggcgccg agctgcagga gcatacccgc gtggtcgagg
      541 tgatccgcga agacggcagc gtccgcggcg tgcgcgtgcg ccggggcgcc ggcgccgagc
      601 aggcgatcca cgccgcgctg gtggtgggcg ccgacgggtc gcactcggcg gtggcgcggg
      661 cgctgggcct gaaccgcacg ccgcgctggc cgcgccgcct gggcatggtc gcgcactacg
      721 caggtgtgcg cggctgcgac gcctggggcg agatgcacgc ggcggcgcac ggctactgcg
      781 gcttggcgcc gctggccgat ggtctggtca acgtcggcat cgtcgtgcgc atgcccggtc
      841 ccgctgcccc gcgcctgagc agcgaagccc gcttcgaggc cgcgctgggc gctttcccct
      901 ggctggccga tcgcatggcg gcggcgcggc gcgtcacgcc ggtgcgcggg gtggggccgc
      961 tggtggtcga tgctgtgcag gccagcgggg ccgggttcgt gctcgtgggt gatgcggcgg
     1021 gcttcttcga cccgttcacc ggcgagggcg tctacaaggc ggtgcgcggg gccgaactgg
     1081 ccgcgccggt gatcctgcgc gcgctggcgg taggcgacct gtcggcgcgc gccctggcgc
     1141 cgtatggcgc ggcccggcgg cgcgagttcc tggccaagga tctcgtctgc cgcatcgtgc
     1201 agggcttcgt gcaggtgccc gcggggctgg actatgtcgc gccgcggctg gccgggcggc
     1261 cccaccacct ggcgatcatg gccggtgtgc tgggcgactt caccgatgcc cgccaggcgc
     1321 tcagcccggc ctatcttttg ggcttgctgc ggccctgacc cgatcgcgac tcggcgcgtt
     1381 aagctcaccg tgcagtcggg atggcttaac tcccggattc ccgccaccac tccgctgcgc
     1441 gtgggtgcga tataatgagg aaaagcatgt cctgaacgga gcgaatgggc tggaggtatg
     1501 gcgatgacga cgcaagcgaa aacgacgacg gcggacgact tgttgaacct gccggatgat
     1561 gggttccggt atgagttggt ggaaggagac ctgcacaaga tgccaccccc cggacgcagg
     1621 catgcccgga ttattgccaa gctcatcgct gcgttggagc agtatctgag cgaacagcgg
     1681 ctgggcgagg tatatgcgga cttcggctat ctgttggccg agaaccctga cactgtgctc
     1741 gcccccgatg tctcctttct gcgccaggag cggctggcag aggccgatcc cgaccgtccc
     1801 tactgggtgg gggcacccga tctggccgtc gaagtggtgt cgccgggtga ccgctacagc
     1861 gaggtgaatg agaaggtggg tcgctggctg gcggcgggta cgctgctcgt gtgggttatc
     1921 gatccgcgcc ggcagcagat cgccgtgcac caggctccga ccaccgtgac caacctgacg
     1981 cttgcggaca cgctcaatgg cggtgacgta ttgcccggct ggacattgcc ggtgcgcgaa
     2041 gtgttcgcgt gagacggaac catgacccgg cgtggcgcaa acgcctgctg ctccgctgaa
     2101 ggaggtagaa accgatgtgc tatgacgata atgcccgccc gcccgtgccg cccggccccg
     2161 gcggagctgc cgagggcgaa gacctcgtgc tgactgccgc cgacggcacc cgctttgccg
     2221 cctatgccgc gcggcccgcc aaccccaccg gcgcgcagat cctgatctac cccgatgtgc
     2281 gcgggctgca ccagttctat aaggacctgg cgctgcgctt cgccgagacc ggtgtgcgcg
     2341 ccgtcgccat cgactacttt ggccgcaccg ccgggctgac cagccgcgcc gagccgttcg
     2401 actatatgcc ccatgtgcag cagatggaac tgccccacgt tttcgccgat acccgcgccg
     2461 cgctcgacta cctgcgcgcg ggggccggtc ccggcaatcg caccttcctg atcggctttt
     2521 gccgcggcgg cagc
//
LOCUS       DASUYC010000390         3590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_946459,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000390 DASUYC010000000
VERSION     DASUYC010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946459"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..2405)
                     /locus_tag="VF276_06470"
     CDS             complement(66..2405)
                     /locus_tag="VF276_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014402861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEX6606543.1"
                     /translation="MLDKMQRYWQAANYLTVGQIYLQANPLLREPLRMEQIKPRLLGH
                     WGTSPALSLIYVHLNRLINERDLDVIYLAGPGHGGPAIVANVYLEGTYTQIYPEITQD
                     VAGMRRLFRQFSTPGGIPSHVSVTTPGSIHEGGELGYVLTHAFGAAFDNPELIVAAVI
                     GDGEAETGPLAGSWQGINFLNPARDGAVLPILNLNGYKIANPTVLGRETNDNLRALIE
                     GHGYEVFFVEGDDPARVHQQFAATLDTCYERIHAIQEEARAKGVTERPRWPAIILRTP
                     KGWTGPKVVDGAPIEGTFRAHQVPLGDVRARPEQLTELEAWMRSYEPDKVFDENGRLI
                     EELAALAPRGLHRMGANPHANGGKVMVTLRMPDFRKYAIEIPRPATLRYESTRVLGQM
                     MRDMFVMNADEANFRLFCPDEVNSNRLGAVFDVETRCFVGKTIEIDDRVSHNGRVMEI
                     LSEHNCEGWLEGYVLTGRHGLFATYEAFAMVAASMVVQHTKWMEEALNLPWRESIASL
                     NILLTSTCWRNDHNGFSHQGPGLIDVMLSKRGTVARIYLPPDSNCLLSVADHCFRSRN
                     YVNLIVIDKQPHLQYLPMDEAIEHCERGASIWEWASNTDGEEPDVVLACAGDVVTLET
                     IAAASWLRKNIPELKVRVVNVVDLMTLFPPAVHPHGMGETDFVNLFTADKHVIFAFHG
                     YARAMHQIIHGRANPDRFHVRGFIEQGTTTTPFDMVMLNKVSRYDLVNDVVNNVAKMR
                     QRRPEINDICDRLIEQARDYTLEHLEDPPEIRNWTWTEE"
     gene            2865..>3590
                     /locus_tag="VF276_06475"
     CDS             2865..>3590
                     /locus_tag="VF276_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX6606544.1"
                     /translation="MRRLRWLSLCCFVLACVLGVSLFAPPARAAGDTLQTAFAQAAQE
                     FGVPPSVLLAVSYNESRWETHHGQPSTAGGYGPMHLVDLSNARLDDARGDGSVHMLKP
                     NPNDPAQQTLATAAALLGVKPDVLKHDAAQNIRGGAALLAQYARDTVGGTPADPAQWY
                     GAVVKYSASSDPEAAFSFADDVYATIQQGAARTTSDGQQVTLAASPVQPDIHSADRLP
                     QPDKHHRPADCPQGLACRFIPAAY"
BASE COUNT          559 a         1139 c         1243 g          649 t
ORIGIN      
        1 aagagtctcg tcgctggggg tacgagacgg agattcttcg ctgcgctcag aatgacgggt
       61 tccggttact cctccgtcca ggtccagttg cggatctcgg gcgggtcttc caggtgctcc
      121 agtgtgtagt cgcgggcctg ctcaattagc cggtcgcaga tgtcgttgat ttccgggcgg
      181 cgctggcgca tcttcgccac gttgttgacc acgtcgttga ccaggtcgta gcggctcacc
      241 ttgttcagca tgaccatgtc gaacggggtg gtcgtggtgc cctgctcgat gaagccgcgc
      301 acgtggaacc ggtcggggtt ggcccgcccg tggatgatct ggtgcatggc gcgggcgtag
      361 ccgtggaagg cgaagatgac gtgcttatcg gccgtgaaga ggttgacgaa atcggtttcg
      421 cccatgccgt gcgggtgaac ggcgggcggg aacagcgtca taaggtccac cacgttgacc
      481 acgcgcacct tgagttcggg gatgttcttg cgcaaccatg aggccgcggc gatggtttcg
      541 agggtcacga catcgcccgc gcaggccagc accacgtccg gctcctcgcc gtcggtgttg
      601 ctggcccatt cccagatcga ggcgccgcgc tcacaatgct cgatggcctc atccatgggc
      661 agatattgca ggtgcggctg cttgtcgatg acgatcaggt tgacgtagtt gcggctgcgg
      721 aagcagtggt cggccaccga cagcaggcag ttggagtcgg gcggcaggta gatacgggcc
      781 accgtgccgc gcttggagag catcacatcg atcaggccgg gcccctggtg gctgaagccg
      841 ttgtggtcgt tgcgccagca ggtcgaggtt agcaggatgt tcagcgaggc aatcgactcg
      901 cgccatggca ggttgagggc ttcttccatc cacttggtgt gttgcacgac catcgaggcc
      961 gccaccatgg cgaaggcttc gtaggtggcg aagaggccgt ggcggccggt cagcacgtag
     1021 ccttccagcc agccctcgca gttgtgctcg ctgaggatct ccatgacgcg accgttgtgc
     1081 gacacgcggt cgtcgatctc gatggtcttg ccgacgaaac agcgggtctc cacgtcgaac
     1141 acggcgccca gccggttgga gttcacctcg tcggggcaga acaggcggaa attggcctcg
     1201 tcggcgttca tcacgaacat gtcgcgcatc atctggccga gcacccgggt cgactcgtag
     1261 cgcagggtgg ccgggcgggg gatctcgatg gcgtacttgc ggaagtcggg catgcgcagc
     1321 gtgaccatca ccttgccgcc gttggcgtgc gggttggcgc ccatgcgatg caggccgcgg
     1381 ggggccaggg cggccagttc ctcgatcagg cggccgttct cgtcgaacac cttgtccggc
     1441 tcgtagctgc gcatccacgc ctctagttcg gtgagttgct ccgggcgcgc gcgcacgtcg
     1501 ccgagcggca cctgatgcgc gcggaaggtg ccctcgatgg gcgcgccgtc caccaccttg
     1561 gggccggtcc agcccttggg cgtgcgcagg atgatggccg gccagcgcgg gcgctcggtg
     1621 acgcccttgg cgcgcgcctc ctcctggatg gcgtggatgc gctcgtagca ggtgtcgagc
     1681 gtggcggcaa actgctggtg cacccgcgcc gggtcgtcgc cttccacgaa gaagacctcg
     1741 tagccgtggc cttcgatcag ggcgcgcagg ttgtcgttgg tctcgcgtcc cagcacggtc
     1801 gggttagcga tcttgtagcc gttgaggttc aggatcggca gcaccgcgcc gtcgcgcgcc
     1861 gggttgagga agttgatgcc ctgccacgaa ccggccagcg gccccgtctc ggcctcgccg
     1921 tcgccgatca cggcggccac gatcaactcc gggttgtcga aggcggcgcc gaaggcatgg
     1981 gtcagcacat agccgagttc accgccctcg tgtatcgagc cgggcgtggt gacgctgaca
     2041 tggctgggga tcccgccggg cgtcgagaac tggcggaaca gccgccgcat cccggccacg
     2101 tcctgggtga tctcggggta gatctgggta taggtgcctt ccaggtagac attggcgacg
     2161 atggccgggc caccgtggcc ggggccggcc aggtagatca cgtcgagatc gcgctcgttg
     2221 atcagccggt tgaggtggac gtagatgagg ctgagcgcgg gcgaggtgcc ccagtggccg
     2281 agcaagcgcg gcttgatctg ctccatacgc agcggttcgc gcaggagcgg gttggcctgg
     2341 agatagatct gcccgaccgt caaatagttg gccgcttgcc aatagcgctg catcttgtcg
     2401 agcatctccg gcgacagcgg cccttcgacc gggccttccg gcttgggcgg cgcgttctcc
     2461 gctttcaccg cgctcgatat ggcggcatcg aattcataca tgcgatactc ctctcttgtc
     2521 cagtcccaga gccggggccg gctgccggac gcccggcgcc ggtgacggtc aggggcgtgt
     2581 acctatccgg cgccagccgg cgcccgctgc tgcgatctgc taatcggccc agtataccat
     2641 tcaacgggtg cgcgcgctac gcgtgcgcgc cgaacggggc tgtttggccg ggcaggtggg
     2701 ccggttgccg atccagggcc acggatcacg ggcacatccg tattcagcaa accgcgtacc
     2761 ctattgtgta acctcatcat agcgcgatat aataacctta ccacacccat tacggggtaa
     2821 tgccgcggcc tatttggatc ttcaggagga tgcacatgat agcgatgcga cgattacgtt
     2881 ggctgagtct ctgctgcttc gtgctggcct gtgtgctggg ggtgagcctg ttcgcgcccc
     2941 cggcgcgcgc cgccggcgat acattacaga ccgcgttcgc gcaggcggcg caggagtttg
     3001 gcgtgccgcc gtcggtgctg ctggcggttt cctacaatga gagccgctgg gagacccacc
     3061 acggccagcc cagcacggcg ggcggctacg gcccgatgca cctggtggac ctgagcaacg
     3121 cgcgcctgga cgatgcccgc ggcgacggca gtgtccatat gctcaagccc aaccccaacg
     3181 atccggcgca gcaaacgctg gcgacagccg ccgcactgct gggggtcaag ccggacgtgc
     3241 tgaagcatga cgcggcgcag aatatccgcg gtggcgcggc gctgctggcc cagtatgcgc
     3301 gcgacaccgt gggcggcacg cccgccgacc cggcccagtg gtacggcgcg gtcgtcaaat
     3361 acagcgcctc cagcgacccg gaagctgcgt tctccttcgc cgatgacgtg tacgcgacga
     3421 tccagcaggg cgccgcccgc acgaccagcg acggccagca ggtgacgctg gcggccagcc
     3481 cggtgcagcc cgacatccac agcgccgacc ggttgccgca gcccgacaag caccatcggc
     3541 ccgccgattg cccgcagggc cttgcttgcc ggttcatccc cgccgcgtat
//
LOCUS       DASUYC010000391         3158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_541111,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000391 DASUYC010000000
VERSION     DASUYC010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541111"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="VF276_06480"
     CDS             <1..652
                     /locus_tag="VF276_06480"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6606545.1"
                     /translation="LPARISLAAAFRLDLRAHRTFAWLVASRFLFLLGTYAVGRFFLY
                     FIADRLGLDPAQAAGEAGGLLAGLTLVTVLAALPAGWAADRWGRLPLMLAGAAVSALG
                     TLGLIVAGGAGQILLFGGLMAVGSAAFAGANWALTADVAPPAEAARFFGLANVGTAGA
                     AAAAGLFGPLIDSANAAAPGLGYTVLFAGATLAFVLSGAVLGFARPAARPSVIFGE"
     gene            719..2290
                     /locus_tag="VF276_06485"
     CDS             719..2290
                     /locus_tag="VF276_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015231629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX6606546.1"
                     /translation="MSGQRGLDLPSRLAYLYAPILPYLKHIKIRRAGPRDFQPADVLV
                     PEGYAVEVVARGLSAPVHCCWDAQGQLYVTEAGHKIESAPRVVKVDAATGATTPYFEF
                     PAERWVQTGAVTGACWHEGALYVMNTDTLSRVNPDGTLEDVLTDLPGRGDHQSNYPVP
                     GPDGKIYFGQGSATNNGVVGADNFAYEWLPKFPAFHDVPAQDVRLTGRNYEFRNVLGD
                     VREKVRTGAYVPFGTETAPGQVISGNVKCSGAILRCNPDGSDLEVVAWGLRNPYGIAF
                     DPQGRLFTTEHGSDERGGRYIVGDVDDFYEIRPGAWYGWPDFASGIPLDDPSWGAGGQ
                     GREPVLAEFPDPHPPRPFVSFSPHSGANGVSFCADPAFGFPGDAFVALFGDVAPITVP
                     RLPVPAGFKVVRVDMQAQQVVDFAVNKIAGPASKLPHAGFERPSHCAFGPDGALYIVD
                     WGEIEIAPEKGGIRMQAGSGAVWRIRRTSAPRGERPPAPIAFPLNAFVALAGLIGIVG
                     FVAGVVWVLRRVLRR"
     gene            complement(2518..2922)
                     /locus_tag="VF276_06490"
     CDS             complement(2518..2922)
                     /locus_tag="VF276_06490"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6606547.1"
                     /translation="MAKKGDLGERLRSLRKARGWTQEELAAASGLTRSHISRLEGGDI
                     QLPSRDRLLRLATALGTTPDDLLAAAGYRVEDRGEGLPDLPVYLRLKYDLDDPRTVAA
                     IAAIVERMREFNAAPPGPPDQEDGVTPAGQMS"
BASE COUNT          456 a         1082 c         1076 g          544 t
ORIGIN      
        1 cctgcccgca cgcatcagcc tggccgccgc tttccgactg gatctgcggg cgcaccgcac
       61 cttcgcctgg ctggtcgcgt cgcgcttcct gtttctgctg ggcacctatg cggtgggtcg
      121 ctttttcctc tacttcatcg ccgaccggct cgggctggat cccgcccagg cggcaggcga
      181 ggccgggggc ctcctggccg gtctgaccct ggtgactgtg ctggccgcgc tgcccgccgg
      241 ctgggccgcc gatcgctggg gccgcctgcc gctgatgctc gccggggcag ccgtctcagc
      301 gctgggcaca ctgggcctga tcgtcgccgg cggtgccggg cagatcctgc tgttcggcgg
      361 tctcatggcc gtcggctcag cggcctttgc cggggcgaac tgggcgctga ccgccgatgt
      421 agcgccgccg gccgaagccg cccgcttctt cggcctggcc aacgtgggca cggccggcgc
      481 ggcggcggcg gccggcctct tcggtccgct aatcgacagc gccaacgcgg cggcgcccgg
      541 cctgggctat actgtgctct ttgccggcgc caccctggcc ttcgtgctca gtggcgccgt
      601 cctcggcttt gcccgacccg ccgcgcggcc aagcgtgatc tttggcgaat gaaggcgccc
      661 cgcacgagca cgcccgcgca gcgggcggcc ggcgcgccat acgtgaaagg agcaacctgt
      721 gagcggtcaa cgcggcctcg atctgcccag ccggctggca tacctgtatg ccccgatcct
      781 gccctatctg aaacatatca agatccggcg ggccggtccg cgcgatttcc agccggccga
      841 tgtcctggta ccggaagggt acgccgtcga agtggtggcc cgcggcctca gcgcgccggt
      901 gcattgttgc tgggacgcgc agggccaact ttacgtcacc gaggccggcc acaaaattga
      961 gtcggcgccg cgggtggtca aggtggacgc cgccaccggc gccaccaccc cctattttga
     1021 gttcccggcc gagcggtggg tgcagaccgg cgcggtgacc ggggcctgct ggcacgaggg
     1081 tgccctgtac gtcatgaaca ccgatacgct ctcgcgcgtg aaccctgacg ggacgctgga
     1141 ggatgtattg acggatctgc cggggcgcgg cgatcaccag agtaactacc cggtccccgg
     1201 ccccgacggc aagatctact tcggccaggg gagcgccacg aacaatgggg tggtcggcgc
     1261 ggacaatttc gcctacgagt ggttgcccaa gttccccgcc ttccacgacg tgccggccca
     1321 ggacgtgcgg ctgaccggcc gcaattacga gtttcgtaac gtgctgggtg acgtgcggga
     1381 gaaggtgcgc acgggcgcgt atgtcccctt cggtaccgag acagcccccg gccaggtgat
     1441 ttcgggtaat gtgaaatgca gcggggcgat cttgcgctgc aaccccgacg gcagcgatct
     1501 ggaggtggtc gcctgggggc tgcgcaatcc ctatggcatc gcgttcgatc cccagggccg
     1561 gctttttacc accgaacacg gcagcgacga gcgggggggc cgctacatcg tcggcgacgt
     1621 ggacgacttt tatgagatcc gccccggcgc gtggtacggc tggcccgact tcgcgtccgg
     1681 catccccctg gatgacccct cctggggcgc gggggggcag gggcgggaac cggtgctggc
     1741 cgagtttccg gatccgcatc caccgcggcc cttcgtcagc ttctccccgc atagcggcgc
     1801 gaacggcgtc agcttctgtg ccgatccggc cttcggcttc cctggcgacg cgtttgttgc
     1861 cctattcggc gatgtcgcgc cgatcaccgt cccgcgcttg ccggtgccgg cgggattcaa
     1921 agtggtgcgc gtggatatgc aggcccagca agtggtggac ttcgccgtca acaaaatcgc
     1981 cggcccggcg tcgaagctgc cccacgcggg gttcgagcgt ccctcgcact gcgcgttcgg
     2041 ccccgacggt gccctctata tcgtcgactg gggcgagatc gagattgcgc cggagaaagg
     2101 cggcatccgc atgcaggccg ggagcggggc cgtgtggcgc atccggcgga ccagcgcacc
     2161 acggggcgaa cgcccgccgg cgcccattgc cttcccgctc aacgccttcg tggcgctcgc
     2221 cgggttgata ggcatcgtgg gcttcgtggc cggagtggtc tgggtactac ggcgggtcct
     2281 gcggcgctaa acggtgcagc ccatgggccg gctcctagcg gagggagcgc tcgcgcaggg
     2341 caaaaaaagg cccctgcttg ggacaggggc cttcgcacca ggaggagttt ttcgtccgtc
     2401 ttatctcatc tgggtttagt atagcatccc gcccccggcg ggggaatcac ccaatggtac
     2461 tgttttggta tggtactctc ggtaaggtcc cgatcaggta ccacccggac catcgggcta
     2521 cgacatttgc cccgccgggg tcacgccgtc ctcctggtcg ggcgggccgg gcggggccgc
     2581 gttgaactcg cgcatgcgct cgacgatggc ggcgatggcc gcgaccgtgc gcgggtcatc
     2641 caggtcgtac ttcaagcgca ggtacaccgg caagtcgggc aggccctcgc cgcggtcctc
     2701 cacgcgatac cctgccgccg ccagcaagtc gtcgggcgtg gtgccgagcg cggtcgccag
     2761 ccgcaacaac cgatcgcgtg agggcagttg gatgtcgccc ccctccaggc gggaaatatg
     2821 gctgcgcgtc aggccgctgg ccgccgccag ttcctcctgg gtccaccccc gcgctttccg
     2881 caacgaccgc aaccgctctc caagatcacc ttttttcgcc accggtctgg ttctcctcct
     2941 cacgggccgc aacgacccgc acgtaacagc gcccaattgt ccgatcacca aattaccacg
     3001 attgcgacgc agagtcaagc tggacagccg tggatgggca ccgcagagga cgcagagata
     3061 agggaatggg cgcgacaggc aagcgtgggg ttgcgctccg cagccactag agccgtgtgt
     3121 gactttatgg gacctgcccg cgaacctgcc ataactgt
//
LOCUS       DASUYC010000392         2295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_180089,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000392 DASUYC010000000
VERSION     DASUYC010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180089"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..158)
                     /locus_tag="VF276_06495"
     CDS             complement(<1..158)
                     /locus_tag="VF276_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEX6606548.1"
                     /translation="MKIVTVVGARPQFIKAAPLSRALRAEPGVQEVLVHTGQHYDDAM
                     SAVFFREL"
     gene            complement(166..1245)
                     /locus_tag="VF276_06500"
     CDS             complement(166..1245)
                     /locus_tag="VF276_06500"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012035481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX6606549.1"
                     /translation="MIPMAKPLIGEEEKQEVLRVLGSGGLAQGSEVKAFEDGFAAALG
                     VKHAVATANGTTALHLALLANGVGPGDEVITVPFTFIASANAVLYCGATPVFVDIEPD
                     SFNMDPGLIEAAITPRTKAILPVHLYGNPADMPRIMAIAERHGLRVIEDACQAHGATI
                     AGRTVGTFGTGCFSFYPTKNITTAEGGIITTDDDEVADLARMLRAHGMRRRYYHEMLG
                     YNFRMTDVHAAIGVAQLPKLAEFTRRRQANAAYLSAHLPADKVRVPVVRPDVAHVFHQ
                     YTVRVLPPLERDAVRAKLAKAGVGSEVYYPLPVHRQQVYLDRGYGDLCFPVTEEATAQ
                     VLSLPVHPALTEQDLAAVVAAVRAL"
     gene            complement(1447..>2295)
                     /locus_tag="VF276_06505"
     CDS             complement(1447..>2295)
                     /locus_tag="VF276_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX6606550.1"
                     /translation="ANGVIVVAGDNEVHVVDAATGRRLFIFGTAAPIYAAPVIANGAI
                     YAPSTDGFLYVYEVPPELIPPAATPTAVATATPTATPTPPPPAALSPVPNPVITGTLY
                     FTATGHTLAPPLRDYWQRYGGLAQFGYPLTEPFTETVTIGPGEGPTHFYLVQYFERAR
                     FEYHPENAGTPSAVLLGLLGVHFYPPEDAALRQTDPGVTYLNGHNLGGVFRAYWEKHG
                     GLFVNGYPISEEHDEVSPTDGKTYRVQYYQRVRMEYHPEYAGTDHEVLLGLLGSQLLR
                     ERGWIR"
BASE COUNT          353 a          733 c          838 g          371 t
ORIGIN      
        1 tccagttcgc ggaaaaagac ggcggacatg gcgtcgtcgt agtgctggcc ggtgtgcacc
       61 agcacctcct gcacgcccgg ctcggcgcgc aacgcccggc tgaggggcgc ggccttgata
      121 aactggggcc gcgcccccac cacggtgacg atcttcattc cgggcctaca gcgcgcgcac
      181 ggcggcgacc acggcggcca ggtcttgctc ggtgagggcc gggtggacgg gcagcgagag
      241 cacctgcgcc gtggcctcct cggtcacggg gaagcagagg tcgccgtagc cgcgatccag
      301 atagacctgc tggcgatgca ccggcagcgg gtaatagact tcgctgccca cgcccgcctt
      361 ggccagcttc gcgcgcacgg cgtcgcgctc cagcggcggc agcacgcgca cggtgtactg
      421 gtggaacaca tgcgccacgt cgggccggac caccggcacc cgcaccttat cggcgggcag
      481 gtgggcgctc aagtacgcgg cgttggcctg gcggcggcgg gtgaactccg ccagcttggg
      541 cagttgggcc acgccgatgg cggcgtgcac gtcggtcatg cggaagttgt agccgagcat
      601 ttcgtggtag tagcggcggc gcatgccgtg ggcgcgcagc atgcgggcca ggtcggccac
      661 ctcgtcgtcg tcggtggtga tgatgccgcc ctcggcggtg gtgatgttct tggtggggta
      721 gaagctgaag cagccggtgc cgaaggtacc cacggtgcgc ccggcgatcg tcgcgccgtg
      781 agcctggcag gcgtcctcga tcacgcgcag accgtggcgc tcggcaatcg ccatgatacg
      841 cggcatatcg gcggggttgc cgtagagatg cacaggcaag atagctttgg tgcgcggggt
      901 aatcgcggcc tcgatcagac cggggtccat attgaagctg tcaggttcga tgtccacgaa
      961 caccggcgtc gccccacaat agagcacggc gttggccgag gcgatgaacg tgaagggcac
     1021 ggtgatgacc tcgtcgccgg gcccgacgcc gttcgccagc agcgccaggt gcagcgccgt
     1081 ggtgccgttg gccgtggcga cggcgtgctt gacgcccagc gccgccgcga agccgtcctc
     1141 gaacgccttc acctcgctgc cctgcgccag cccgcccgag ccgagtaccc gcagcacctc
     1201 ttgtttttct tcctcgccga tgagcggctt tgccatcgga atcatagcag cgttactcct
     1261 tacttgcgtc gcaaaccggg gcgccggcgc cattgcccgg tcgcccccaa tcacccggac
     1321 gatgggatcc gatctccggg cgcttaagta taccgccaaa tcgtccgccg ccaaaggacg
     1381 cgccgcgcgg caggggccgg tttgcgaccg gcccccgcgt gacgtgtacc taggcacagg
     1441 acaaggtcag cggatccagc cgcgctcccg cagcagttgg ctgcccagca ggccgagcaa
     1501 tacctcgtga tccgtcccgg cgtattcggg gtgatattcc atgcgcaccc gctggtagta
     1561 ctggacccgg tacgttttgc cgtcggtagg cgagacttcg tcgtgctcct cggagatggg
     1621 gtagccgttg acgaacaggc cgccgtgttt ctcccagtag gcacggaaca cgccgccgag
     1681 attatggccg ttgaggtagg tcacgccggg gtcggtttgg cgcagcgcgg catcttctgg
     1741 tggatagaaa tggacgccga gcaggccgag cagcaccgcg ctgggcgtcc cggcgttttc
     1801 ggggtggtac tcgaagcgcg cccgctcgaa atactggacc aggtagaaat gggtcgggcc
     1861 ctcgccgggg ccgatggtca cggtctcggt gaagggctcg gtcagcggat agccgaactg
     1921 ggccaggccg ccgtagcgct gccagtagtc gcgcaggggc ggggccagcg tgtggccggt
     1981 ggcggtgaaa tagagcgtgc cggtaatcac cgggttgggc accgggctca gggcggcggg
     2041 cggtggcggc gtgggcgtgg ccgtgggcgt ggccgtggct actgccgtag gcgtggccgc
     2101 cggcgggatc agttcgggcg gcacttcgta gacgtacagg aagccgtcgg tggacggcgc
     2161 atagatcgcg ccgttggcga tgaccggcgc ggcgtagatc ggcgccgcgg tgccgaaaat
     2221 gaagaggcgg cgcccggtcg cggcgtcgac cacgtgaacc tcgttgtccc cggccacgac
     2281 gatcacgccg ttggc
//
LOCUS       DASUYC010000393         3446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1387966, whole genome shotgun sequence.
ACCESSION   DASUYC010000393 DASUYC010000000
VERSION     DASUYC010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1387966"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..1146)
                     /locus_tag="VF276_06510"
     CDS             complement(31..1146)
                     /locus_tag="VF276_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX6606551.1"
                     /translation="MPESIQRPARLLIVDDQAANIRLLECTLQEIAGLTLVTTTDSTQ
                     ALPLYLAVRPDAILLDLRMPGLDGFTILEQLRPLVPANEYLPILVISADITPDAKLRA
                     LALGATEFLSKPYNPREVELRVRKMLEARFLHQQLEIQNQVLEQLVSERTRALEEAQV
                     EILERLALAAEYRDDDTGQHTQRVGHLAALLAQELGLGRGQVELIRRAAPLHDVGKIG
                     VPDHILLKPGKLTPAEYAQIKLHTTIGAEILSGGRSELIRTAQQIALTHHERWDGTGY
                     PARLAGEAIPAAGRIVSVADVFDALIHARPYKPAWPVEQAVAEIRRQSGAQFDPAIVA
                     AFLRVQDTLGGPEALLEAPPAFGRPHAVPAYRAGIPA"
     gene            complement(1691..3322)
                     /locus_tag="VF276_06515"
     CDS             complement(1691..3322)
                     /locus_tag="VF276_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606552.1"
                     /translation="MTGKTRGLVRWVAGGIVGMALVAGAVASPPASLAGPPPPQAAPT
                     GQAATGVAGTIYYGAVPPGGGMNKPVIVFVQGLHGQASGWWTNGNDMYADAYNAGFRT
                     AFVDLTDASGTGGSIWTNGRMLAGQLGTITAHYGVSSVNVVAHSKGGLDTNAAIVHYG
                     AGPRVQTLFQLASPNWGSQLADLSYSWWGGWLAAILGQRDDAVYSLQTGQMSYFRSVT
                     DSRAENGYVHYATSAGTSHGSLFGKLWWGGAYLQPYGDNDGAVTLTSAHLPLGTHVFT
                     DSRLDHDGMAVGHNTWSRVLPYATSLWRGAAPAQQPPPARAPVAAPDPYAALNAADII
                     RGGPLAGRAVERFPVESGAQALNVAVLAPGPLTVSLHAPDGRRYRPTAVSSGAANVPL
                     GTATQHIFQIAAPLAGEWQAEIGAQAPVALARPAPPGAYLLVAEIQSDLRVDLTRPAG
                     PPTFAPGAAPQFQVAARDRAGSLAGVQTEVHLSGDKGAPGSTTRQGATPRVALPTAPG
                     VYNLSLTVRGLDRRGLPFERSLTTSFGVAALPAQP"
BASE COUNT          494 a         1198 c         1218 g          536 t
ORIGIN      
        1 acaaggggcg gggacggcgc ccgtcgcggc tcaggccgga atgcccgccc ggtaggcggg
       61 gacggcgtgc gggcggccaa acgcgggcgg cgcctccagc agcgcctcgg ggccgcccag
      121 cgtgtcttgc acgcgcaaga aagccgcgac gatggccggg tcaaactgcg ccccgctctg
      181 ccggcgaatc tcggccaccg cctgctccac cggccaggcg ggcttgtagg gccgggcgtg
      241 gatcagcgcg tcgaacacat cggccacgct gacgatgcgc cccgccgcgg gaatggcctc
      301 gcccgccagt cgcgccgggt agccggtgcc gtcccagcgc tcgtggtgcg tcaacgcgat
      361 ctgctgcgcc gtgcggatca gttccgaacg cccgcccgac aggatctcgg caccgatggt
      421 cgtgtgcagc ttgatctggg catattccgc cggggtcaat ttgcccggtt tgagcaaaat
      481 atgatcgggc acgccgattt tgcccacatc atgcagcggc gcggcccggc ggatcagttc
      541 gacctggccg cggcccaggc ccaattcctg cgccagcagg gcggccaggt ggcccacacg
      601 ctgcgtgtgc tggccggtgt cgtcgtcgcg atactcggcg gccagcgcca gccgttccag
      661 gatctccacc tgcgcctctt ccagcgcccg cgtgcgctcg gagaccagct gttccagcac
      721 ctggttctgg atctccaact gctgatggag aaaacgcgct tccagcatct tacgcacgcg
      781 caactcgact tcgcgcggat tatagggctt ggacagaaac tcggtggcac ccagcgccag
      841 cgcgcgcagc ttggcgtcgg gggtgatgtc ggcgctgatc accaggatcg gcagatattc
      901 gttggccggg accagcgggc gcagttgctc caggatggtg aacccgtcga ggccgggcat
      961 gcgcaggtcg agcaggatgg cgtccgggcg caccgccaga tagagcggta gcgcctgcgt
     1021 cgaatcggtg gtcgtgacca gtgtcaggcc cgcgatctcc tgcaacgtgc attccaacag
     1081 gcgaatattt gccgcctgat cgtcgacgat cagcagccgc gcgggacgct ggatggactc
     1141 cggcatgttc gctccacgat atttccgacg gcgaccgcgc cgtcctgacc ggcgatgtat
     1201 ggcgggtggg gtcttgcggg caagaccggc ggggccgtat cagcgcctcc aggcccctgg
     1261 tgcggggacc cgacacggac accgaatcca ggatacggaa tcgcgcaacc cggcgcaatg
     1321 gtctcctggc ccgccccggc agggaccatc gggcacactg gcgcaccgcc gggcatcgcc
     1381 gggcgcccgc cacgggcgcg ccccctcacc cttgtcactc tgagcgcagc gaagagtctc
     1441 ggcgctggtc cgccagacgg aaattcttcg ccctgctcac aatgacgatg gttgcttgga
     1501 tttgtcactc tgagggcagt gaagagtctc gtcgttgcgc ctcttacgag acatattctt
     1561 cgctccgctc agaatgacga tggatgctga ggtttgtcac tctgagcgca gcgaagagtc
     1621 tcgtcgcagc atccccacga gacgagattc ttcgctccgc tcagaatgac ggtacgccgg
     1681 ggccgcctgc tcagggctgc gcgggcaggg cggccacgcc gaagctggtg gtcaggctgc
     1741 gctcgaacgg caggccacgc cggtccaggc cgcgcacggt caggctgagg ttgtagacgc
     1801 cgggcgcggt gggcagcgcc acgcgcggcg tcgcgccctg gcgcgtggtg gaaccgggcg
     1861 cccctttgtc gccgctcagg tggacttcgg tctgcacgcc ggccaggctc ccggcgcggt
     1921 cacgcgcggc cacctggaac tgcggcgcgg cgccgggtgc gaaggtgggc gggccggcgg
     1981 ggcgagtcaa atccacgcgc agatcgctct ggatctcggc caccagcagg taggcgccgg
     2041 gcggcgcggg gcgggccagg gccacgggcg cctgggcgcc gatctcggcc tgccactcgc
     2101 ccgccagggg cgcggcgatc tggaagatat gctgcgtggc ggtgcccagc ggcacgttgg
     2161 cggcgccgct gctcacggca gtggggcgat accggcgtcc gtcgggggcg tgcaggctga
     2221 cggtcagcgg ccccggcgcc agcacggcca cgttcagcgc ctgggcgccg ctctcgaccg
     2281 ggaagcgttc cacggcccgg cccgccagcg gcccgccgcg gatgatgtcg gcggcgttga
     2341 gcgcggcata gggatcgggc gccgcgaccg gcgcgcgcgc cgggggcggc tgttgcgccg
     2401 gggccgcccc gcgccagagg ctggtggcat agggcagcac ccggctccag gtgttgtggc
     2461 ccacggccat gccgtcatgg tccagccggc tgtcggtgaa gacgtgggtg ccgagcggga
     2521 gatgggcgct ggtcagggtc acggcgccgt cgttgtcgcc gtagggttgc aggtacgccc
     2581 cgccccacca cagcttgccg aacagcgacc cgtggctggt gcccgcgctg gtggcgtagt
     2641 gcacatagcc gttctcggcg cggctgtcgg tgacgctgcg gaagtagctc atctggccgg
     2701 tttgcagcga gtagaccgcg tcgtcgcgct ggcccaggat ggccgccagc cacccgcccc
     2761 accagctata cgacaggtcg gccagttgcg acccccagtt gggcgaggcc agttggaaca
     2821 gcgtctgcac gcgcggcccc gcgccgtaat gcacgatggc cgcgttggtg tcgaggccgc
     2881 ccttgctgtg cgccacgacg ttgacgctgc tcacgccgta gtgggcggtg atggtgccca
     2941 actgcccggc cagcatccgc ccgttggtcc agatgctgcc gccggtgccg ctcgcgtcag
     3001 tcaggtcaac gaaggcggtg cggaaaccgg cgttgtaggc gtcggcatac atgtcgttgc
     3061 cgttcgtcca ccagccgctg gcctggccgt gcagcccctg gacgaagacg atcaccggct
     3121 tgttcatgcc cccgcccggc ggaacggcgc cgtagtagat cgtgcccgcc acgccggtcg
     3181 ccgcctgccc ggtcggcgcc gcctgcgggg gcggcggccc ggccagacta gccggcggac
     3241 tggcaaccgc gcctgccacc agtgccatcc ccacgatgcc gcccgcgacc caacgcacca
     3301 gccctcgcgt ctttcctgtc atggtggacc cccttgtgcc gcttgcccat gatccgttac
     3361 cgcgcgccct tgcgcgccat tctactatgc aacctcagca attgcaatag gcccgcctgt
     3421 caggcgttta tctgcaccgc agagac
//
LOCUS       DASUYC010000394         2586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_2051175, whole genome shotgun sequence.
ACCESSION   DASUYC010000394 DASUYC010000000
VERSION     DASUYC010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2051175"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2586
                     /locus_tag="VF276_06520"
     CDS             <1..>2586
                     /locus_tag="VF276_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002637901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6606553.1"
                     /translation="ILELKNTINTMVDQLNSFASEVTRVAREVGTEGKLGGQAQVKGV
                     GGTWKDLTDNVNLMAGNLTSQVRNIAAVTTAVANGDLSKKITVDAKGEILELKNTINT
                     MVDQLNAFASEVTRVAREVGTEGKLGGQAEVRGVGGTWKDLTDNVNYMAANLTNQVRG
                     IARVVTAVANGDLKRKLVLDAKGEIAELADTINGMIDTLATFAAQVTTVAREVGTEGK
                     LGGQANVPGAAGTWRDLTDNVNWLAANLTTEVRAIAEVATAVTKGDLSRSITVEAAGE
                     VAALKDNINEMIRNLKDTTQKNMEQDWLKTNLAKFTRLLQGQKDLLNVSRLILSELAP
                     LVSVQHGIFFINDPVDGEAELRLLASYGYKERKSLSSRFHIGEGLVGQSVYEKERILL
                     TDVPSDYVKINSGLGEATPLNIVVLPVLFEGQVMAVIELASFQRFSDTHLNFLDQLTE
                     SIGIVLNTIAANTRTEELLKQSQSLTQELQTQQAELTETNRRLEQQAKSLQASEEQLR
                     QQQEELQVTNEELEEKARLLSSQNIEVERKNQEIELARQALEEKAEQLAITSRYKSEF
                     LANMSHELRTPLNSMLILSKLLAENPDGNLTAKQVEFAQTIHGAGTDLLVLVSDILDL
                     SKIESGTISVDAGRVAFAALGDTLTRIFQPVAEAKGLAFACDLRPGLPDYIYTDSKRL
                     EQVLKNLLSNGIKFTEQGQVSLEIGPVTHGWSRDRDTLNRADAVVAFTVRDTGIGIPA
                     DKHRVIFEAFQQADGTTSRRYGGTGLGLSISREIAHLLGGEIRLVSKPGEGSTFTLYL
                     PQAYPGSRAIGGNGAEPAAEEHTESAPARGGRRAVRGRAARGANGGTPAGTAMAAETP
                     MFEES"
BASE COUNT          533 a          846 c          872 g          335 t
ORIGIN      
        1 agatcctcga actcaaaaac acgatcaaca cgatggtcga ccagttgaac tcgttcgcgt
       61 cggaagtgac gcgcgtggcg cgggaggtgg gcaccgaggg caagctgggc gggcaggcgc
      121 aggtcaaagg cgtcggcggc acgtggaagg atctgaccga caacgtgaac ctgatggccg
      181 ggaacctgac cagccaggtg cgcaacatcg cagcggtgac cacggcggtc gccaacgggg
      241 atctctcgaa gaagatcacg gtggacgcga agggcgagat cctcgaactc aagaacacga
      301 tcaacaccat ggtggaccag ctcaacgcct tcgcgtcgga agtaacgcgc gtggcccgcg
      361 aggtgggcac ggagggcaag ctgggcgggc aggccgaagt gcgcggcgtt ggcggcacgt
      421 ggaaagacct gaccgacaac gtgaactaca tggccgccaa cctgaccaac caggtgcgtg
      481 gcatcgcccg cgtggtcacg gcggtggcca acggcgacct gaagcgcaag ctggtgctcg
      541 acgccaaggg cgagatcgcc gaactggccg acacgatcaa cggcatgatc gacacgctgg
      601 cgaccttcgc cgcccaggtg acgactgtgg cgcgcgaggt gggcaccgag ggcaagctcg
      661 gcgggcaggc caacgtgccc ggcgccgcgg gcacctggcg cgacctcacc gacaacgtga
      721 actggctggc cgccaacctg accaccgaag tgcgcgccat cgccgaggtc gcaacggcgg
      781 tgaccaaggg cgacctgtcg cgctcgatca ccgtggaggc ggcgggcgag gtggccgcgc
      841 tcaaagacaa catcaacgag atgatccgca atctcaagga caccacccag aagaacatgg
      901 agcaggactg gttgaagacc aacctggcca agttcacccg gctgctgcaa ggccagaagg
      961 acctgctcaa cgtgtcgcgg ctcatcttgt cggaactggc gccgctggtg tcggtgcaac
     1021 acggcatctt ctttatcaac gacccggtgg atggcgaagc cgaactgcgg ttgctggcga
     1081 gctacggcta caaggagcgc aagagcctgt cgagccgctt ccatatcggc gagggcctgg
     1141 tgggccagag cgtctacgag aaagagcgca tcctgctgac cgatgtgccg tcggactacg
     1201 tgaagatcaa ctcggggctg ggcgaggcca ccccgctgaa catcgtcgtc ctgccggtgc
     1261 tgttcgaggg ccaagtgatg gcggtcatcg aactggcctc gttccagcgc ttcagcgaca
     1321 cgcacctgaa cttcctcgat cagctcaccg agagcatcgg catcgtgctc aataccatcg
     1381 ccgccaacac gcgcaccgag gagttgctga agcaatcgca gtcgctgacc caggaactgc
     1441 agacgcagca agccgaactg accgagacca accggcggct ggagcagcag gccaagtcgc
     1501 tgcaagcctc cgaagagcaa ctgcgccagc agcaagaaga gttgcaggtg accaacgagg
     1561 aactggaaga gaaagcgcgg ctgctctcca gccagaacat cgaggtggag cgcaagaacc
     1621 aggagatcga gttggcccgc caggcactgg aggaaaaggc cgagcaactg gcgatcacct
     1681 cgcgctacaa gtccgagttc ctcgccaaca tgtcgcacga gttgcgcacg ccgctcaaca
     1741 gcatgctcat cctgtccaag ctgctggccg agaacccgga cggcaacctg acggccaagc
     1801 aggtcgagtt cgcccagacc atccacggcg cgggcaccga cctgttggtg ctggtcagcg
     1861 acatcctcga cctgtccaag atcgagtcgg gcacgatctc ggtggacgcc gggcgggtgg
     1921 ccttcgcggc gctgggcgac acgctgaccc gcatcttcca gccggtggcc gaggcgaagg
     1981 ggctggcctt cgcctgcgac ctgcggccgg ggctgcccga ttacatctac accgactcca
     2041 agcgcctgga gcaggtgctc aagaacctgc tctccaacgg catcaagttc accgaacagg
     2101 gccaggtctc gctggagatc gggccggtga cgcacggctg gagccgcgac cgggacacgc
     2161 tgaaccgcgc cgacgcggtc gtcgccttca ccgtgcgcga caccgggatc ggcatccccg
     2221 ccgacaagca ccgcgtcatc ttcgaagcgt tccagcaagc cgacggcacg accagccgcc
     2281 gctacggcgg cacggggctg ggcctgtcga tcagccgcga gatcgcccac ctgctgggcg
     2341 gggaaatccg gctggtgagc aagcccggcg aggggagcac gttcacgctc tacctgccgc
     2401 aggcctatcc tggctcgcgc gccatcggcg gcaacggcgc cgagcccgcc gcggaggagc
     2461 acaccgagtc cgccccggcc cgcggcgggc ggcgggcggt gcggggccgc gcggcgcgcg
     2521 gcgcgaacgg cggcaccccc gccggcaccg ccatggccgc cgagacgccg atgttcgagg
     2581 agagcg
//
LOCUS       DASUYC010000395         2573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_765405,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000395 DASUYC010000000
VERSION     DASUYC010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765405"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1669)
                     /gene="ftsH"
                     /locus_tag="VF276_06525"
     CDS             complement(<1..1669)
                     /gene="ftsH"
                     /locus_tag="VF276_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX6606554.1"
                     /translation="MGDNKWLRNSFVYVIIMVAVLVLLFAFLNPKNSDTTISISQVAK
                     DIQRGDVESLKTQAESDQVLVKYKAGHQPLTAITNKEAGSLTDTLTALGVTKDQLAAV
                     NISVEPASSWGSWLGTLGFILPTLFLIGVFVFMMRQAQGTNNQALSFGKSRARLFMGN
                     KPTITFSDVAGVEEAKQELAEVVEFLKYPDKFAALGARIPRGVLLVGPPGTGKTLLAR
                     AVAGEAGVPFFSISGSEFVEMFVGVGASRVRDLFDQAKRNAPCIVFVDEIDAVGRQRG
                     AGLGGSHDEREQTLNQILVEMDGFDSQTNIIVVAATNRPDVLDPALLRPGRFDRQVVL
                     DRPDIKGRVAILQVHSRGKPLEKGVSLETLAKQTPGFSGADLENLVNEAAILAARRNR
                     KTIGMNELEEAIDRVIAGPERKSRVMSEKEKKVIAYHEGGHTLVGHVLPNADPIHKVS
                     IIARGRALGWTLALPTEDKHMRTRSELRDELAMLLGGRTAEELIFADPTTGAQNDIEK
                     CSQIARAMVTEYGMTDALGPQQLGYRNGEPFLGRDMGASRDYSDEVAS"
     gene            complement(1929..2486)
                     /gene="hpt"
                     /locus_tag="VF276_06530"
     CDS             complement(1929..2486)
                     /gene="hpt"
                     /locus_tag="VF276_06530"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX6606555.1"
                     /translation="MWYDTTIEKVLIDDAAVQAKIRELGTQITADYAGKDLLLVGVLR
                     GALLFIVDLARQIDLPLEMDFMAISSYGAATETSGVVRILKDLDTAIEGRHVLIVEDI
                     IDSGLTLNYIVEHLRNRNPASIKICALLNKPSRRRVDVRIDYHGFDIPDEFVVGYGLD
                     YRQRYRNVPFVGVLKPETYTAHPEE"
BASE COUNT          442 a          838 c          800 g          493 t
ORIGIN      
        1 ggctcgccac ctcgtcggag tagtcgcggg aggcgcccat gtcgcggccg aggaacggct
       61 cgccgttgcg gtagccgagc tgctgcggcc cgagggcgtc ggtcatgccg tactcggtga
      121 ccatggcccg ggcgatctgg ctgcacttct cgatgtcgtt ctgggcgccg gtcgtggggt
      181 cggcgaatat cagctcctcg gccgtccgcc cgccgagcag catcgccagc tcgtcgcgca
      241 gctccgagcg ggtgcgcatg tgcttgtcct cggtgggcag cgccagcgtc cagcccagcg
      301 cccggcccct ggcgatgatc gagaccttgt ggatcgggtc ggcgttgggg aggacgtggc
      361 cgaccagcgt gtggccgccc tcgtggtagg cgatgacctt cttctccttc tccgacatca
      421 cccggctctt gcgctccggc ccggcgatga cgcggtcgat ggcctcttcc agttcgttca
      481 tgccgatagt cttgcgattg cggcgggcgg ccaggatcgc ggcctcgttg accaggttct
      541 ccaggtcggc acccgagaag cccggcgtct gcttggcaag cgtctccagc gacacgccct
      601 tctccagggg tttgccccgg ctgtggacct gcaagatcgc cacgcggcct ttgatgtcgg
      661 gccggtccag gaccacctgg cggtcgaagc gaccagggcg caacagcgcc gggtccagca
      721 cgtcgggccg gttagtggcg gcgaccacga tgatgttggt ctggctgtcg aagccgtcca
      781 tctcgaccag gatctggttc agcgtctgct cgcgctcgtc gtgcgaacca ccgaggcccg
      841 cgccgcgctg gcgcccgacg gcgtcgatct cgtccacgaa gacgatgcag ggcgcgttgc
      901 gcttggcctg gtcgaacagg tcgcgcacgc ggctggcgcc gacgccgacg aacatctcga
      961 caaactccga gccgctgatg gagaagaacg gcaccccggc ctcgccggcc acggcgcggg
     1021 ccagcagcgt cttaccggtg cccggagggc cgaccagcaa cacgccgcgc gggatgcgcg
     1081 ccccgagtgc ggcgaacttg tcggggtatt tgaggaactc gaccacctcg gccagttcct
     1141 gcttggcctc ctccacgccc gccacgtcgc tgaaggtaat ggtcggctta ttgcccatga
     1201 acaggcgtgc ccggctctta ccgaacgaca gggcctggtt attcgtgccc tgggcctggc
     1261 gcatcatgaa gacgaacacg ccgatcagga acagcgtcgg caggataaag ccgagcgtcc
     1321 ccagccacga tccccaggag gatgccggct ccacgctgat gttcaccgcg gccaactggt
     1381 ccttggtgac gccgagggcg gtcagcgtat cggtcaggct ccccgcctct ttgttggtga
     1441 tcgcggtcag cggctgatgg cccgccttgt acttgaccag aacctggtcg ctctccgcct
     1501 gcgtcttcag gctttccaca tctccacgct gtatatcctt cgccacttgc gaaatggaga
     1561 tggttgtgtc gctgttcttg gggttaagaa aagcgaacag cagcaccagc accgccacca
     1621 taatgatgac gtagacgaag ctgttgcgga gccacttatt gtctcccatg tatattcctc
     1681 cagggggatg tttcctcaac ggaaacgcaa tccgaaccgt cagagatgtc tgtgtgggcg
     1741 cagcgcaaaa aggaccgcac cacgcgggca ccaccggctg gcgtaaactc attataccaa
     1801 agcgtgcgca gacctgtcaa aagttcacac cgccttcagc aacccgtgat tccttacctt
     1861 gcatatagat acgtagcctg ccttatcatg gattgtgcca gcggcggatt cgcgcgcgca
     1921 gttttgggct attcttccgg atgcgcggta tacgtttccg gttttagaac tcccacaaac
     1981 ggcacattgc gatagcgctg ccggtaatcc agcccataac cgacgacaaa ctcgtcgggg
     2041 atatcaaacc cgtggtagtc gatcctcaca tccacgcggc gccgggatgg cttgttcagc
     2101 agcgcgcaga tcttgatgct ggccgggttg cggttgcgca ggtgctcgac gatgtaattg
     2161 agggtcagcc cgctgtcgat gatatcctcg acgatcagga cgtgccggcc ctcgatggcc
     2221 gtgtccaggt ccttgaggat gcgcaccacg ccgctggtct ccgtggcggc gccgtaggag
     2281 gagatggcca tgaaatccat ctccagcggc aaatcgatct gccgggccag atcgacgata
     2341 aacagcagcg cgccccgcag cacgccgacc agcagcaggt ccttgcccgc gtagtccgcc
     2401 gtgatctgcg tccccagttc acgaattttc gcctgcaccg cggcatcatc gatcagcact
     2461 ttctcaatcg tcgtgtcata ccacatgcgt ccagctttcc tcctgtataa ccatgggcca
     2521 atagcgcgat ccggccggat cgtggattac gcgggcacga aggtaaaggc gcg
//
LOCUS       DASUYC010000396         4285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1940162, whole genome shotgun sequence.
ACCESSION   DASUYC010000396 DASUYC010000000
VERSION     DASUYC010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1940162"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VF276_06535"
     CDS             complement(<1..534)
                     /locus_tag="VF276_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016875661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX6606556.1"
                     /translation="MKIPLKRYWALLATYLRPQRARVGLLAGLLLLNIGLELLNPQIV
                     RYFIDTALAGGALETLAMAAGAFIGIALVTQGLNVWATYLSENIAWTATNALRLDLTA
                     HCLRLDLSFHKARTPGELIERIDGDVTALSNFFSQLVIHLLGNVLLLVGVLALLFTVD
                     WRVGLAVTGFALLALVIL"
     gene            complement(903..1340)
                     /gene="mutT"
                     /locus_tag="VF276_06540"
     CDS             complement(903..1340)
                     /gene="mutT"
                     /locus_tag="VF276_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007632760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HEX6606557.1"
                     /translation="MPSPSTPRNITVAAAIIRQDGRILIVQRPETAEMGGLWEFPGGK
                     IEPGETPQAALVREIAEELGVPVAVGDLYHTTRHTYPRGLTVHLLFYECRLLGGPLQL
                     LWGQDYRWVAPAALPTYAYPAADQEVVARLAATEQTPPIPESE"
     gene            complement(1349..1804)
                     /locus_tag="VF276_06545"
     CDS             complement(1349..1804)
                     /locus_tag="VF276_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606558.1"
                     /translation="MPDSVQAGITWGRFVLALRYGFLCGVGILVLQIVMELIIDHHYD
                     VSNSSLAIAAGLNCTAISFFRSLALPVESPVVRRVLHALTLGTVEFVIILFSDVVRKS
                     LSGESLLFPDLGFPLLLALGFAVFCFMVEPTLVERPKNAITSGDPNPHT"
     gene            complement(1850..2338)
                     /gene="paaD"
                     /locus_tag="VF276_06550"
     CDS             complement(1850..2338)
                     /gene="paaD"
                     /locus_tag="VF276_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HEX6606559.1"
                     /translation="MTQHTTITEADVVAALREVYDPEIPNLSLVDLGIYRGAEITPDG
                     VTIEITPTFVGCPALDYMRDQIVERCAELWPGTPVQVKVTLDRPWTSDQISEEGRRRL
                     KVSGFAPPPKGNLIRLEPLVECPYCASRNTVLENAFGPTLCRAIYYCKNCRQPFEQFK
                     AV"
     gene            complement(2335..3606)
                     /locus_tag="VF276_06555"
     CDS             complement(2335..3606)
                     /locus_tag="VF276_06555"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6606560.1"
                     /translation="MTTQTATPRLNRGLAWLRTPVGRGFLLLIVATAATGFAMAAQQN
                     IVANYFEGVLGLDGAQFGYITAIREIPGFLLIFLTALFYRLSLPRLTALALLVLAVGY
                     GLFGWSNSFWTVAPWVIISSMGYHTWLQTQYALGMSLTTEARSGGILGRLSAINSAGG
                     LAAMVVVLVGFGQGWLDFRLTFVVCGVLALVAAVAIFGFPHLHNGEVQAQAARRQPIV
                     WRKEYRYYYLLSLLDGGRQQIFFSFGLWVLVHHFALDVPTVSAILLTVTSLSMVAGSW
                     IGRLIDRHGERQMLALVNVAYVVALLGYALIDNLAVVVVCYVIYSFIFPLSAIGAATY
                     LRKVAVASEIAPSLAMGVTLQHAAAIVVPIATGFVLNYVGYQLPFLVACVFACFTFGV
                     TRRLSPETQKSPRRVQEDALRVGAESLEVSP"
     gene            complement(3611..>4285)
                     /gene="paaC"
                     /locus_tag="VF276_06560"
     CDS             complement(3611..>4285)
                     /gene="paaC"
                     /locus_tag="VF276_06560"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: protein motif:HMM:TIGR02158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HEX6606561.1"
                     /translation="WTGIAPMLEEDVAYSSMAQDEIGHARLFYTLLAERVGVDADALA
                     YGRPEAGFRNAQFLEMPRTNWAFTLVRQFLYDQYDQVRLEALAASKLTALAEVIGKIQ
                     REEKYHAMHGTTWMRRLAENTPEARARLDAVLPAAWAATAGLFEPLPGEDLLVRADYL
                     GQSSADLQAAWTERVGGVLRELGVTVPDVPPTGMGGRSGRHSAAFAPMWDEMTIVYRI
                     DPAARW"
BASE COUNT          865 a         1360 c         1406 g          654 t
ORIGIN      
        1 caggatgacc agcgccagca gcgcaaaccc ggtgactgcc agccccaccc gccaatccac
       61 ggtaaacagc agggccagta cacctacgag cagcagcacg ttgccgagca ggtggatcac
      121 cagttgggag aagaagttgg acagcgcagt gacgtcgccg tcgatgcgct cgatcagttc
      181 gccgggtgtg cgcgccttgt ggaacgacag gtccagccgc aggcagtgcg cggtcaggtc
      241 gaggcgcagg gcattggtcg cggtccaggc gatgttctcg ctcaggtagg tggcccagac
      301 gttcagcccc tgcgtgacca gagcgatgcc gatgaacgcg cccgcggcca tggccagcgt
      361 ctccagcgcc ccgcccgcca gcgccgtgtc gatgaagtag cgcacgatct gcgggtttag
      421 caactctagc ccgatgttga gcaagagcag cccggccagc agccccacgc gggcgcgctg
      481 cggccgcaaa taggtggcaa gcaatgccca atagcgtttc agcgggattt tcacgaatcg
      541 ctccgatagc aaagccaaaa tacgagaatc gctacactca cggacctgtc attctgagcg
      601 cagcgaagaa tctccgtctc gtacccgccg gcgacgagac tcttcgctcc gctcagagtg
      661 acaacataat ccaacgacga gactcttcgc tccgctcaga gtgacaacga gcgcattcgc
      721 ccgtagccct gtcattctga gcgcagcgaa gaatctccgt ctcgtacccc gcaggcgacg
      781 agactcttcg ctcccctcag agtgacaaca aggtaaaccc accgcaagca tacccgcgtg
      841 ggtatgcttc atattatcac ggctatggcg cggcggggaa atagtctgga gatggactta
      901 ccctactccg attcgggtat aggcggggtt tgttcggtgg cggcgaggcg ggcgaccact
      961 tcctggtcgg cggcggggta ggcgtaggtg ggcagggcgg cgggcgcgac ccagcggtag
     1021 tcctggcccc acagcaattg caacggcccg cccagcaggc ggcactcata aaacaggaga
     1081 tggacggtca gcccgcgcgg gtaggtatgg cgcgtcgtgt ggtacagatc gcccacggcc
     1141 accggcacgc ccagctcctc ggcgatctcg cgcaccaggg cggcctgcgg cgtctcgccc
     1201 ggctcgatct tgccgccggg gaactcccat agcccgccca tctccgccgt ctccggccgc
     1261 tgcacgatca ggatgcgccc gtcctggcgg atgatcgcgg cggccacggt gatgttgcgc
     1321 ggagtagatg gtgaaggcaa agatctactc acgtatgcgg gttgggatcg cccgaagtga
     1381 tcgcgttctt cgggcgctct acgagagtag gctcgaccat aaaacagaac acagcgaagc
     1441 cgagcgccag cagcagaggg aatccgagat caggaaacag taaactctct ccagataatg
     1501 acttgcggac tacgtcgctg aacagaataa taacaaactc cacggttcct aaggtgagag
     1561 catgaagtac acgtctcaca accggagact caaccggtaa agctaatgat cgaaagaatg
     1621 aaatagcggt acagtttaat ccggcagcta tggcaaggct gctgttggag acatcatagt
     1681 gatggtctat aattagctcc atcactattt gcaacactaa aataccaaca ccgcagagaa
     1741 aaccatatcg caacgcaagc acaaaccgcc cccatgttat accggcttgc acagaatcgg
     1801 gcatagcaaa gctccctaca tgcaatagga taggtgcacg gattagcccc tacaccgcct
     1861 tgaactgctc gaaaggctgg cggcagttct tgcagtagta gatggcgcgg cagagcgtgg
     1921 ggccgaacgc gttttcgagc accgtgttgc gcgaggcgca gtaggggcac tcgaccagcg
     1981 gctccaggcg aatcaagttg cccttgggcg gcggggcgaa gccggagacc ttgaggcggc
     2041 ggcggccctc ctcgctgatc tggtcgctgg tccagggccg gtcgagcgtg accttgacct
     2101 gcacgggcgt gcccggccag agttccgcac aacgctcgac gatctggtcc cgcatgtagt
     2161 cgagcgccgg gcagccgacg aaagtgggcg tgatctcgat ggtgacgccg tcgggcgtga
     2221 tctccgcacc gcggtagatg ccgaggtcga ccagcgagag gttggggatc tccgggtcgt
     2281 atacctcgcg caaggcggcg acgacatcgg cttcggtgat agtggtgtgt tgggtcatgg
     2341 cgatacctct agactttcgg cgcccacgcg cagggcgtct tcctgcacac ggcgcggcga
     2401 cttctgcgtt tcgggcgaca accggcgcgt cacgccgaag gtgaagcagg cgaacacgca
     2461 ggccaccagg aatgggagtt gatagccgac atagttaagc acaaagccgg tggcgatggg
     2521 cacgacaatc gcggcggcgt gctgcaacgt gacgcccatc gccagcgacg gcgcgatttc
     2581 ggacgcaaca gccactttgc gcagataggt cgccgcgccg atggccgaca atgggaagat
     2641 gaacgagtag atgacgtagc agacgacgac cacggccagg ttgtcgatca acgcgtagcc
     2701 gagcagggcg acgacatagg ccacgttgac cagcgccagc atctgccgct cgccgtggcg
     2761 gtcgatcagc cgcccgatcc acgacccggc gaccatgctc aggctggtga cggtcagcag
     2821 aatggccgag acggtgggca catcgagggc gaagtgatgc accagcaccc acagcccgaa
     2881 cgagaaaaag atctgctgcc gcccgccgtc gagcaggctc agcaggtagt agtagcgata
     2941 ctccttgcgc cagacgatgg gctggcggcg cgcggcctgc gcctggacct cgccgttgtg
     3001 caggtgcggg aagccaaaga tcgccaccgc ggcgaccagc gccagcacgc cgcagaccac
     3061 gaacgtcagc cggaagtcga gccagccctg gccgaagccg accagcacca cgaccatggc
     3121 cgccagcccg cccgcactgt tgatggccga gaggcggccc aggatgccgc cggagcgtgc
     3181 ctccgtggtc aggctcatgc ccagggcgta ctgcgtttgg agccaggtgt ggtagcccat
     3241 cgaactgatg atgacccagg gcgcgaccgt ccagaacgag ttggaccagc cgaacaggcc
     3301 gtagcccacg gccagcacca gcagggccag agccgtgagg cgcggcaaag agagccggta
     3361 aaatagcgcg gtcaggaaga tgagcaggaa gccggggatc tcgcggatgg ccgtgatgta
     3421 gccgaactgc gcgccgtcca gccccagcac gccctcgaaa tagttggcga cgatgttctg
     3481 ctgcgcggcc atggcgaagc cggtggcggc ggtcgccacg atcagcagca ggaagccgcg
     3541 tccgaccggg gtgcgcagcc aggcgaggcc gcggttcagg cgcggcgtgg cggtttgcgt
     3601 cgtcatcggc tcaccagcgc gccgccgggt cgatgcggta gacgatggtc atctcgtccc
     3661 acatcggggc aaaggcggcg gagtgccggc cagagcgccc acccatcccg gtagggggca
     3721 cgtcgggcac ggtcacgccg agctcgcgca ggacgccgcc cacgcgctcg gtccaggccg
     3781 cctggagatc ggcgcttgat tggcccaggt agtccgcgcg caccagcaga tcctcgccgg
     3841 gcagcggctc gaacagcccc gccgtggccg cccaggcggc gggcagcacg gcgtccagcc
     3901 gggcgcgcgc ctcgggcgta ttctcggcca ggcggcgcat ccaggtcgtg ccgtgcatgg
     3961 cgtggtactt ctcctcgcgc tggatcttgc cgatgacctc ggccagcgcc gtcagcttcg
     4021 atgcggccag cgcctccagc cgcacctgat cgtactggtc gtagaggaac tggcgcacca
     4081 gggtgaaggc ccagttggtg cgcggcatct ccagaaactg cgcgttgcgg aagcccgcct
     4141 ccgggcggcc ataggccagc gcgtcggcgt ccacgcccac ccgctcggcc agcagcgtat
     4201 agaacagccg ggcgtgcccg atctcgtcct gggccatcga gctataggcc acgtcctctt
     4261 cgagcatcgg cgcgatgccg gtcca
//
LOCUS       DASUYC010000397         5838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1537452, whole genome shotgun sequence.
ACCESSION   DASUYC010000397 DASUYC010000000
VERSION     DASUYC010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5838
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1537452"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..1737
                     /locus_tag="VF276_06565"
     CDS             226..1737
                     /locus_tag="VF276_06565"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEX6606562.1"
                     /translation="MQEPLRQLREILAEVSDLNRAAAVLGWDQETYMPPGGVRARAEQ
                     LATLRRISHVRFTSDEVGALLDTLAETTAGDDPASDDASLVRVTKRDYDKARKLPPEL
                     VAEISRAGALARPAWIEARKKSEFSIFAPFLQTNIDLNKRVADTLGYDEHPYDALIDR
                     SEPGMTAAQLRRLFDELKAAIVPLVRAIAQQGDVVDTSFLHREYDEQQQLAFGLKVVE
                     QYGYDLGRGREDLTTHPFCTSFSPDDVRITTRVSRDFLPMALFGTMHESGHGMYEQGV
                     NPALDRSPLCRGASPGVHESQSRLWENLVGRSRAFWQGYFPKLQAAFPDQLRDVDMER
                     FYRAVNKVTPSTIRVEADEVTYNLHILLRFELELALLEDKLHVNEVQDAWNARMQDYL
                     GITPPNDAEGVLQDIHWSSGGLGGFSGYTLGNIIGAQLFAKAREALPDLDAQIAAGEF
                     GPLHEWLRTNLYQHGRKFTPNELLERITGGPLSTAPWIAYVRRKFGDIYHLAD"
     gene            1867..2217
                     /locus_tag="VF276_06570"
     CDS             1867..2217
                     /locus_tag="VF276_06570"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006675589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX6606563.1"
                     /translation="MARSFRYAFAGLFYVVRSQRNFRIHLAAALVAAALALALGFTPV
                     EWAVLATIITLVMTLEMVNTVVEAAVDLASPAYHPLAKIAKDVAAGAVLLTAIGAVVV
                     GLFLFVPHLLGLIR"
     gene            complement(2766..5111)
                     /locus_tag="VF276_06575"
     CDS             complement(2766..5111)
                     /locus_tag="VF276_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606564.1"
                     /translation="MVHQTRHPRRVLAGIVALLALLALAGWQIGQTHPAAPGATLGRG
                     PLGSSATGAGAGVPNGPKPPEVAANGPIIVGQSSKNDVSPPLRDMKPLPAINKGEQET
                     NQRNEWIPLIGHKDVPDPVVQHSFGASADPNAPVPNIPAPVKNWAGINQAGGCGGCAP
                     PDTNGDVGPNHYVQTVNSSFQIWNKSGTSLYGPAAINTIWSGFGGACQTRNDGDPVVL
                     YDPLADRWLISQFTAASPYNECIAISSTSDPTGTWNRYAFQLSTSDFPDYPHLGVWPD
                     GYYMSVNWFTGGQTYAGPRPYVFNRTAMINGTAATFQTTSAALGSSAAPIHPADFDGT
                     TLPPSGAPDTFVQFGSTLKLYKFHVDWTTPANTTWTNSANLTAAGFTQLCSSTQNCVP
                     QPSTTQKLDGLGDRLMHRVAYRNFGDHESLVLSHSVSAGGVSGVRWYEVRNPSSTASL
                     YQQGTYQPDTTWRWMGSVAMNKAGDMALGYSASSSSVYPSIRYTGRLATDALGTMPQG
                     EGTILAGSGYQSGVNRWGDYSAMTVDPTDDCTFWYTQEYNNSGGWGWSTRIGAFQVST
                     CSGSPPPPTATNTPTRTPTPVPGVTNTPTRTPTRTPTQVPGATNTPTRTPTRTATPVP
                     SGCAEALTNGGFESGTSPWVQTSSGGYQLIDTTRPHTGAYSVYLGGYNNGTDTIYQQV
                     AVPGTATSATLTYWWYMTSAEGTTTAYDKLTTQVLNSSGTVLATLKTISNVNTRNTWV
                     KETFDLTAYKGQTIRVYFKGTTDSSLLTSFFVDDVSLNVCQ"
BASE COUNT          881 a         1926 c         2012 g         1019 t
ORIGIN      
        1 aattggcgcg tccgtagccg ttatttccat tagtcgtatt cttcgcgctc ttcgcgcctt
       61 cgcggtgcgc ccctcgcatt cttcgcgtcc ttcgcgcctt cgcggtgaaa cagaatcggc
      121 ccgcgtggtt gtctgcccgg cgcaaagcat gctacaatgg cgggaccaat ctgatgccgg
      181 cggggcgggg tccacgaccc gcccccgcgg aaggagaccg aatccatgca agaaccgctg
      241 cgccaactgc gcgagatcct ggctgaagtg agcgacctga accgggccgc cgccgtgctg
      301 ggctgggacc aggaaaccta tatgccgccg ggcggggtgc gggcgcgcgc cgagcaactg
      361 gcgaccctgc gccgcatctc ccacgtgcgc ttcacctccg acgaggtggg ggcgctgctg
      421 gacacgctgg ccgagaccac cgccggggac gacccggcgt ccgacgacgc cagcctggtc
      481 cgcgtgacca agcgcgacta cgacaaggcg cgcaagctgc cgcccgaact ggtggccgag
      541 atctcgcggg ccggcgccct ggcgcgcccg gcctggatcg aggcgcgcaa gaagtccgag
      601 ttctcgatct tcgcgccctt cctgcaaacc aacatcgacc tgaacaagcg cgtggccgac
      661 accctgggct acgacgagca cccctacgac gcgctgatcg accgcagcga gccgggcatg
      721 accgcggcgc agttgcgcag gctgttcgac gagctcaagg cggccatcgt gccgctggtg
      781 cgcgccatcg cccagcaggg cgacgtggtc gataccagct tcctgcaccg cgaatacgac
      841 gagcagcagc aactcgcctt cggcttgaag gtggtcgagc agtacggcta cgacctgggt
      901 cggggccgcg aggacctgac cacgcacccc ttctgcacca gcttctcgcc cgacgacgtg
      961 cgcatcacca cccgcgtgag ccgcgacttc ctgcccatgg cgctgttcgg cacgatgcac
     1021 gagagcggcc acggcatgta cgagcagggc gtcaatcccg cgctcgaccg cagccccctc
     1081 tgccggggcg cctcgccggg cgtccacgag tcgcagtcgc ggctgtggga gaacctggtg
     1141 ggccgcagtc gcgccttctg gcagggctac ttccccaagc tgcaagccgc cttccccgac
     1201 cagttgcgcg acgtggacat ggagcggttc taccgcgccg tgaacaaggt cacgccgtcc
     1261 accatccgcg tcgaggccga cgaggtgacg tacaacctgc acatcctgct gcgcttcgag
     1321 ttggaactgg cgctgctgga agacaagctg cacgtgaacg aggtgcagga cgcctggaac
     1381 gcgcgcatgc aggactacct gggcatcacg ccgcccaacg acgccgaggg cgtgctgcaa
     1441 gacatccact ggtcgagcgg cgggctgggc ggcttctccg gctacacgct gggcaacatc
     1501 atcggggcgc aactcttcgc caaggcccgc gaggccctgc ccgacctgga cgcccagatc
     1561 gcggcgggcg agttcgggcc gctgcacgag tggctgcgga cgaatctgta tcagcacggg
     1621 cgcaagttca cgccgaacga gttgctggag cgcatcaccg gcgggccgct gagcaccgcg
     1681 ccctggatcg cctacgtgcg ccgcaagttc ggcgacatct accacctggc cgactagccg
     1741 ggcccgtcgc gcgccgccat gaccccgccg tctctgtcgc cgcaccggcc cgacgccccc
     1801 gccccggttc ccgaccccgc ctctgccgct agcgaggcac cgccggagcc ggggcgggcg
     1861 ggcacgctgg cccgctcgtt ccgctacgcc ttcgccgggc tgttctacgt ggtgcgcagc
     1921 cagcgcaact tccgcatcca cctggcggcg gcgctggtgg cggcggcgct ggcgctggcg
     1981 ctcggcttca cgccggtgga atgggccgtc ctcgccacga tcatcaccct ggtcatgacc
     2041 ctggagatgg tcaacacggt ggtcgaagcc gccgtggacc tcgccagccc ggcctaccac
     2101 cccctcgcca agatcgccaa ggacgtagcg gcgggcgccg tcctgctcac ggccatcggc
     2161 gcggtggtgg tgggcctgtt cctgttcgtg ccgcacttgc tggggttgat ccgttgatgc
     2221 ggcgcatgtc ggaaaaggaa aacttcccac ctcccgaagc aagaaaacaa tacgcgcggc
     2281 gcttggtggg ttggggtagc gcgctcttga tcatatccgc cggcgtaacc cttattggcc
     2341 ggctgacgcg cctgggcggg tgcctcacgg agttcgctat cgctattatg gccgttagta
     2401 tcggcgttct ggggctgggg ctgttcggct attgggcagt ggttcgcctc aaggcaccgt
     2461 ggttctacaa gttacaggat atgtgggggc gcctattcca cgaagaaaag ggagtgtaag
     2521 ctattctaat tgtcattctg agcgcagcga agaatctccg tctcgtgcgg gatggcgccg
     2581 agactcttcg ctgcgctcag agtgacaatt tcttgcgtta ctgaccactg accactgacc
     2641 actgaccact cgtcgtcact cagcactcag cactcagcac tcgaaagggc cgggcacacg
     2701 ccgttgtgta cccggccccg gatggtgcga cctggacccg gtgggccggg gcaggccgcg
     2761 ctagcctact ggcagacgtt caggctcacg tcgtccacga agaagctggt gagcaggctg
     2821 gagtccgtcg tccccttgaa gtaaacgcgg atggtctggc ctttgtacgc cgtgagatcg
     2881 aaggtttcct tcacccaggt gttgcgggtg ttgacgttgc tgattgtctt cagcgtcgcc
     2941 agcaccgtgc cgctgctgtt gagcacctgc gtcgtcagct tgtcgtaggc cgtcgtggtg
     3001 ccttccgcac tggtcatgta ccaccagtag gtcagcgtgg ccgacgtagc ggtgccgggc
     3061 acggccacct gctggtagat ggtgtcggtg ccgttgttgt agccgcccag gtagacgctg
     3121 taggcgccgg tgtgcgggcg ggtggtgtcg atcagttggt agccgccgct ggaggtttgc
     3181 acccagggcg acgtgccgct ctcgaagccg ccgttggtca gggcttcggc gcagccgctc
     3241 ggcaccgggg tggccgtgcg ggtcggggtg cgcgtcggcg tgttggtcgc gccgggaacc
     3301 tgcgtcggcg tgcgcgtcgg cgtgcgcgtc ggcgtattgg tcacacccgg caccggggtc
     3361 ggcgtcctgg tcggcgtgtt ggttgccgtc ggcggcggcg ggctgccgga gcaggtggag
     3421 acctggaacg cgccgatgcg cgtcgaccag ccccagccgc cgctgttgtt gtattcctgc
     3481 gtataccaga aggtgcagtc atcggtcggg tcgaccgtca tggcactgta gtcgccccag
     3541 cggttcacgc ccgactggta gccgctgccg gccaggatcg tgccctcacc ctgcggcatg
     3601 gtgcccaggg catcggtcgc caggcggccg gtgtagcgga tgctgggata gacgctgctg
     3661 ctggaagcgc tgtagcccag ggccatgtcg ccggccttgt tcatggcgac gctacccatc
     3721 cagcgccagg tggtgtcggg ctggtaggtg ccctgctgat agaggctggc cgtgctgctg
     3781 gggttacgaa cctcatacca gcgcacgccg gaaaccccgc ccgcgctgac gctgtggctc
     3841 agcaccagcg actcgtggtc gccgaagttg cgataggcca cgcggtgcat cagccggtcg
     3901 cccaggccgt cgagcttctg cgtggtggac ggctggggca cgcagttttg cgtgctggag
     3961 cacaactggg tgaacccggc ggcggtcaga ttggccgagt tggtccaggt ggtgttggcc
     4021 ggggtggtcc agtccacatg gaacttatag agcttgaggg tgctgccgaa ctgcacgaag
     4081 gtatccgggg cgccgctcgg cggcagggtg gtgccgtcga agtccgccgg gtggatgggg
     4141 gcggcgctgc tgcccagcgc ggcgctggtg gtctggaagg tggccgccgt gccgttgatc
     4201 atcgcggtgc ggttgaagac atagggccgc gggccggcat aggtctggcc accggtgaac
     4261 cagttgaccg acatgtaata cccgtcgggc cagacgccca ggtgcgggta gtccgggaag
     4321 tcgctggtgc tcaactggaa ggcgtagcgg ttccacgtgc cggtcgggtc gctggtgctc
     4381 gaaatggcga tgcactcatt atagggcgag gcggcggtga actggctgat cagccagcgg
     4441 tcggccagcg ggtcgtaaag cacgaccggg tcgccgtcgt tgcgggtctg gcacgcgccg
     4501 ccgaagccgc tccagatggt gttgatggcg gcggggccgt agagcgacgt gccgctcttg
     4561 ttccagatct ggaacgacga gttgacggtc tgcacgtagt gattcgggcc gacatcgccg
     4621 ttggtatcgg gcggggcgca cccgccgcag ccgccggcct ggttgatccc ggcccagttc
     4681 ttgacgggag cggggatatt cgggacgggg gcattcgggt cggcgcttgc gccgaagctg
     4741 tgctgcacga cggggtcggg cacatccttg tgaccgatca acggaatcca ctcgttgcgc
     4801 tggttggttt cttgctcccc tttgttgatc gccgggagcg gcttcatgtc gcgcaacggc
     4861 ggcgacacat cattcttgga cgattgcccg acgatgatcg ggccgttagc ggcgacttcg
     4921 ggcggcttgg ggccgttggg cacccctgcc ccggctcccg tggcgctgct gcccagcggc
     4981 ccccggccca gcgtggcgcc gggcgcggcg gggtgggtct ggccgatttg ccaaccggcc
     5041 agcgcgagca acgccagcaa ggcgacgatg cctgcgagta cccggcgggg atgacgtgtc
     5101 tggtggacca taggtgcggt tcctcctcct taaagacgcg ataccagcgg gtgcgaagac
     5161 caatacggat atgtcgctcg aaggcgcccg cccgtggccg caaccgggcc ggcccggcgc
     5221 cggcctcctc ccggcgcccc gctctcccga gctagacagg aattttcccc gcccctatcg
     5281 tactatagtc gtactgacaa aagtactgac aaaaccggac caatttgaga tgaaatgcgg
     5341 cccggtcgcg atccattttg ccgcgcgtcc ccttcgcgca tcccctctac ttataatatc
     5401 cattttccgg gccaaaccct gccacggctc gttcgttgat cctgtggcag cccgaatccg
     5461 cgccctgccg gcgggcgagc aacctctccc cccgcccctc cccgccacgg ggagaggagt
     5521 gacgttcctg cgcgccgcgc agttccccct tcccggcgcg ggaagggggg caggggggtt
     5581 aggccggtcc ccccaccccg gatttggaac ggggcgcgca atctggtatg ctgccccggt
     5641 acgctgcaac gacgacatga ccgggtcgcc ccggcgcggg acggccctga cgatggagcc
     5701 gacatgaaat atctatgcgc gggcctggtc ctgatcgggc tggctctgct gatggccgtg
     5761 gccccggatg ggcgggcgca ggagggcggg cgctactacc gcgaaaccgg ccacaccctg
     5821 gccgccccct tcgtggcc
//
LOCUS       DASUYC010000398         2919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_221796,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000398 DASUYC010000000
VERSION     DASUYC010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221796"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1278
                     /locus_tag="VF276_06580"
     CDS             <1..1278
                     /locus_tag="VF276_06580"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEX6606565.1"
                     /translation="GGARRRIPPTPMSKPALSGEQITAIKDPRVVEARELATAAGRAR
                     HGKALLEGAASLGWALDARATIEQVFYHAAAREDPVLARLVAVGVPCAAVSEGIAKKI
                     SDTAYLVPLLGVVALPPEREAPAGDFVLVLDHVQDHGNLGTIVRTAGAFGIRDLVATH
                     PELDLYFKKTVAASRGKVFDVRLRRFPSGVAAVQDLKARGYQIVATSPHARDIQALAP
                     LQRRPVALVVGNETAGVGDDILALADVVVQIPMSGAVESLNVGVATGISVYELKVRMV
                     LAMLVNYIRANLGREVNVTGKLIQQAFDAQLRRVSDLNSTQVILLMMLACDESIPEAQ
                     VARDTATFGDDLAALLAPLFARGLIARLPDAVPPAIRITEAGERTLAQLWSVVERADA
                     AIVAGFSDAEKAQLHAFLQRIQANCANVMAGSA"
     gene            complement(1438..2586)
                     /locus_tag="VF276_06585"
     CDS             complement(1438..2586)
                     /locus_tag="VF276_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606566.1"
                     /translation="MSVDESVALLGQQATALLRAQWGADVVLETPIVLKDATRNRIVR
                     YAVAGAALPGRQSVIVKQITREAARGFTDWAGLAFLTGVPGTAGLSPRFYAGDPAAGF
                     FVMQDMGGSRSLDDVLRGDDPAAVWTGFLALARQTARLHGLTLNREAAFTQARADLPE
                     AGALTRHAEAARWQAGLDGVYDWLAAVDCPAPAGLAACCARIAGVYADPGPFLTFTHG
                     DMAPTNNHLVDGHISLVDFEYGAFRHALYDMTCWQMLCPLPEDLVAAISATYRAALAP
                     YCPAVQDADGYAAAWADLCAYRGLAMLQWIPPGILEANRSWVDDWTMREAVLATVEQL
                     REVSAPFPDRAPLTAAAAALAQTLRRRWPEFTTVLPRWPALRLQGYRM"
BASE COUNT          493 a         1035 c          954 g          437 t
ORIGIN      
        1 ggcggcgcgc gccgccgcat cccccccacc ccgatgagca aaccggccct ctccggcgaa
       61 cagatcaccg cgatcaagga cccgcgcgtg gtcgaagccc gcgaactcgc gacggcggcg
      121 ggccgggcgc gccacggcaa ggctctgctc gaaggcgccg ccagcctcgg ctgggcgctg
      181 gacgccaggg ccacgatcga acaggtgttt taccatgccg ccgcccgcga ggaccccgtg
      241 ctggcccggc tggtcgcggt gggcgtgccc tgcgccgccg tgtccgaggg catcgcgaag
      301 aagatcagcg atacggcgta cctggtgccg ctgctcggcg tggtggcgct gccgccggag
      361 cgggaggcgc ccgcgggcga cttcgtgctg gttctcgacc atgtgcagga ccacggcaac
      421 ctgggcacca tcgtgcgcac agccggcgcg ttcggcatcc gcgacctggt ggcgacccac
      481 cccgagctcg atctctactt caagaagacc gtggcggctt cgcgcggtaa ggtgttcgac
      541 gtgcgcctgc ggcggttccc gtcgggcgtg gccgccgtgc aagacctgaa ggcgcgcggc
      601 taccagatcg tggcgaccag cccccacgcc cgcgacatcc aggccctggc gccgttgcag
      661 cgcaggccgg tcgccctggt cgtcggcaac gagacggcgg gcgtgggcga cgacatcctg
      721 gcgctggccg atgtggtggt gcagatcccc atgagcgggg cggtggagtc gctcaatgtc
      781 ggcgttgcca ccggcatcag cgtctatgaa ctgaaggtaa ggatggtcct ggcgatgtta
      841 gtgaactata tccgcgccaa cctgggccgc gaagtcaacg tcaccggcaa gctgatccag
      901 caggcgtttg acgcccaact gcgccgcgtc tccgacctca acagcaccca ggtgatcctg
      961 ctgatgatgc tggcctgcga cgagtccatc cctgaagccc aggtggcccg cgacacggcc
     1021 accttcggcg acgacctggc cgccctgctg gccccgctct tcgccagggg cctgatcgcc
     1081 cggctgccgg acgccgtgcc gcccgccatc cgcatcaccg aggcaggaga gcgcacgctg
     1141 gcccagctct ggagcgtcgt cgagcgcgcc gacgcggcca tcgtcgccgg gttctccgat
     1201 gccgagaagg cccaactgca cgccttcctg cagcgcatcc aggccaactg cgcgaacgtc
     1261 atggccgggt ccgcctagtc acgctgttcc gcgcgggtgt aacccgcgtg ggggccgata
     1321 tccgggccta tcgcagtatt cggcagggcg aaagcggaga gtcacggtgc tggagtcgag
     1381 acgtgatctg gtccccgccc aggggacgtg ctcccgtact cccgcaccgt gattcattta
     1441 catacggtaa ccctggagtc gcaaagcagg ccagcgcggc aaaacggtgg tgaactccgg
     1501 ccagcgcctg cggagcgtct gcgccagcgc cgccgccgcc gcggtgagcg gggcgcgatc
     1561 agggaacggg gcgctgacct cgcgtagttg ctccacggtc gccaacacgg cttcgcgcat
     1621 cgtccaatca tctacccagg atcggttcgc ctccaggatg ccgggcggga tccactggag
     1681 catcgccagc ccgcgatagg cgcagagatc cgcccaggcg gcggcgtagc catcggcatc
     1741 ctgcaccgcc ggacagtatg gggcaagcgc ggcgcgatag gtcgcgctga tcgccgctac
     1801 cagatcctcc ggcagcgggc acaacatctg ccagcacgtc atgtcataca aggcgtggcg
     1861 aaaggccccg tactcaaaat cgaccaggga gatgtgtccg tccaccagat gattgttggt
     1921 gggcgccata tcgccgtgcg taaacgtcag gaatggaccg gggtcggcgt atacgccggc
     1981 gatccgggcg cagcaggcag caagtccggc cggagccgga cagtccacgg cggccagcca
     2041 gtcatacacc ccgtccagcc ccgcctgcca ccgcgccgct tccgcatggc gggtgagcgc
     2101 gccggcctcg ggcagatccg ctcgggcttg cgtgaaggcc gcttcgcggt tgagcgtcag
     2161 gccgtgcagc cgggccgtct ggcgggccag ggcgagaaac ccggtccaga ccgctgccgg
     2221 gtcgtcgccg cgcaacacgt cgtccaggct gcgcgagccg cccatatcct gcatcacgaa
     2281 aaatcccgcc gcgggatcgc ccgcatagaa gcggggactc aagcccgcgg tccccggcac
     2341 cccggtcaaa aaggccagac cggcccaatc cgtgaacccg cgcgccgcct cgcgcgtgat
     2401 ctgcttcacg atcaccgact gcctacctgg gagcgcggcg ccggccaccg catagcgcac
     2461 gatccggttg cgggtcgcat ctttgagcac gatcggcgtt tccagaacga cgtctgcgcc
     2521 ccattgcgcc cgcagcaacg ccgtggcctg ctgccccagc agcgccacac tttcatctac
     2581 actcataaat tcccttgctg gagaggcgat cgagcgctga tacccgatgc tgaccataag
     2641 ccgacggacc cggcaggaga cccgctcgta cagcccaaac ttgaagcgca gaaccccggc
     2701 cctgccggcc cgccacgcca atttgctcga atgggatcat cgcctgatct tccgccaccc
     2761 gcgggcgcac gacgcagccg ggcgacgggg gttactcata ttctgcgcca tgataacaca
     2821 atgaccgcgg gtctgtaaat ttgccacctt tctaccgtat actgggatta gcttcgccgg
     2881 aagttatggc gcatggatga tcccagcccc gctgttcaa
//
LOCUS       DASUYC010000399         2024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1558516, whole genome shotgun sequence.
ACCESSION   DASUYC010000399 DASUYC010000000
VERSION     DASUYC010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1558516"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VF276_06590"
     CDS             <1..431
                     /locus_tag="VF276_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606567.1"
                     /translation="FYAARTLVPGGSFDLTTTGEMTNFAYRDYTLPLLQKQQDEADAG
                     QLSTVNYSDIKVKLLDWNAQATDHGGIATVEVTRTSHVTRPGKSEEVQTGTYQFRLHR
                     HTGDNGEPFWVAVDFLSPISNQWVSESAGMSGPVPPSGHG"
     gene            505..1440
                     /locus_tag="VF276_06595"
     CDS             505..1440
                     /locus_tag="VF276_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX6606568.1"
                     /translation="MLTTPSGDPAGAAFASIPALDAALAAQGYIAGRALATALYLSWR
                     LPKPLFLEGEAGVGKTAVARTLATLLGTPLIHLQCYEGLDISTAVYEWNYARQMMQIR
                     LLEAGHKEATLDTAHLFGPDFLIERPILAAVTYQGPGRPVLLIDELDRADEEFEAFLL
                     ELLADFQVTIPEVGTFRATQPPAVVITSNRTREIHDALKRRCIYHWIDYPTFDKELAV
                     LTTRLPDLPQRLATGIVALVQEIRRLDLYKLPGVAETLDWAAALLALEQQTLTVEVVS
                     DTLGVLLKHQEDIEQLRGELTPLVQKAEARGRAGG"
     gene            1421..>2024
                     /locus_tag="VF276_06600"
     CDS             1421..>2024
                     /locus_tag="VF276_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606569.1"
                     /translation="MDALGDKLVRRWIDFGRLLRRNGVQTTADQMHDLLSVLSDPTQD
                     WTDRQTIYYTARALLCSRHEDWPRFDLIFRQFWGRTRQVIIPSDSATPRPETQLARER
                     ASQALGPDTPGGDMLPSVERTTAIDAPDLPGGETDNSTLPDQMLLYSGYERLRQIDFA
                     DFTDDELAQARAMLATWRWEPGTRRTRRLTPARRGHRLDVE"
BASE COUNT          358 a          753 c          624 g          289 t
ORIGIN      
        1 aattctacgc ggcccgcaca ctggtgcccg gcggcagctt cgacctgacc accaccggcg
       61 agatgaccaa cttcgcctac cgcgattaca cgctgccctt actgcaaaaa cagcaggacg
      121 aagccgacgc gggccaattg agtacggtca actacagcga catcaaggtc aagctgctcg
      181 actggaacgc ccaggccacc gaccacggcg gcatcgcgac cgtcgaggtg acacgcacgt
      241 cccacgtcac gcggcccggc aagtctgagg aggtccagac cggcacctac cagttccggc
      301 tgcaccgcca cacgggcgac aacggcgaac ccttctgggt ggccgtcgat ttcctgagtc
      361 cgatcagcaa ccagtgggtt tcggagagtg ccggaatgag cgggccggtt ccgccatccg
      421 gccatggctg atgagcatcc cgccggccgt gtgccggcat gacccgcggc tccccggtgg
      481 gccgcaaagg aggcgcccgc cctcgtgctg acaacccctt ccggcgatcc cgccggggca
      541 gccttcgcgt cgatccccgc cctggacgcg gctctagccg cccagggcta catcgccggg
      601 cgggccctgg cgaccgccct ctatctgagt tggcggctgc ccaaaccgct gtttctcgaa
      661 ggcgaggccg gcgtcggcaa aacggccgtc gcccgcaccc tggctacgct gcttggcacg
      721 ccgttgatcc acctgcaatg ctacgaagga ctggacatca gcaccgcggt ctatgagtgg
      781 aactacgccc gccagatgat gcagatccgc ctgctcgaag ccgggcacaa agaggccacc
      841 ctcgacaccg cccacctctt cggccctgac ttcctgatcg agcgccccat cctggccgcc
      901 gtcacatacc aggggccggg ccgcccggtg ctgctcatcg acgaactcga ccgcgccgac
      961 gaagagttcg aggccttcct gctggagttg ctggccgact tccaggtcac gatccccgaa
     1021 gtcggcacct tccgcgcgac ccagccgccc gccgtcgtca tcaccagcaa ccgcacccgc
     1081 gagatccacg acgccctgaa acggcgctgc atctatcact ggatcgacta tcccaccttc
     1141 gacaaggaac tggccgtgct gaccacgcgc ctgcctgatc tgccgcagcg gctggcgacg
     1201 ggcatcgtcg cgctggtcca ggagatccgc cggctcgatc tctacaaact acccggcgtg
     1261 gccgagaccc tcgattgggc cgccgccctg ctggccctgg aacagcagac gctcacggtc
     1321 gaagtcgtca gcgacacgct gggtgtgctg ctgaagcacc aggaggacat cgagcagctg
     1381 cgcggtgagt tgacgccgct cgtgcagaag gcggaagccc gtggacgcgc tgggggataa
     1441 actcgttcgc cgctggatcg acttcgggcg gctgttgcgg cgcaacggcg tgcagaccac
     1501 cgcggaccag atgcacgacc tgctcagcgt gctgagcgac cctacccagg actggacgga
     1561 ccgccagacg atctactaca cggcgcgggc actgctgtgc agccgccacg aggactggcc
     1621 gcgcttcgac ctgatcttcc gccagttctg gggccgcacc cgccaggtga tcattccctc
     1681 cgacagcgcc acgccgcgcc cggagaccca actggcccgc gagcgtgcga gccaggccct
     1741 ggggccggac acgcccggtg gcgacatgct gcctagcgtc gagcgcacca cggccattga
     1801 cgcgcccgat ctgcccggcg gggaaacgga caacagcaca ctgcccgatc agatgctgct
     1861 ctacagcggc tacgagcggt tgcgccagat cgactttgcc gacttcaccg acgacgaact
     1921 ggcccaggcg cgcgccatgc tggccacctg gcgctgggaa cccggcacgc gccgcacccg
     1981 ccgcctgacc ccggcccgcc gcgggcaccg gctcgacgtg gagc
//
LOCUS       DASUYC010000400         2399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_772540,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000400 DASUYC010000000
VERSION     DASUYC010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772540"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="VF276_06605"
     CDS             complement(<1..819)
                     /locus_tag="VF276_06605"
                     /inference="COORDINATES: protein motif:HMM:NF013400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606570.1"
                     /translation="MTLRSLSRLPSKLARLRPAALGLLVLLTACSALGGGPPPTATPV
                     WPVVTVAAQPEPVRTQHIEIGLAQGGESFDPTFLHPTIPLIKALGIRYVRLDHIYNHP
                     GVVARRPGGGLTYDWTPLDTLVDDIRAMGAEPMLDLSYMPPALNPQSQLLPPLDWEEW
                     NKLVHTTARHFNVDRALGLRYWEIWNEPNLWGAWQGSYPQYLHLYDISRRAIHDLDPR
                     AVVGGPSLSLYEETALDWLLGYETAQDDGGDVGFLSWHAYGWTPQEMAAQVQAAR"
     gene            complement(901..1509)
                     /locus_tag="VF276_06610"
     CDS             complement(901..1509)
                     /locus_tag="VF276_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606571.1"
                     /translation="MSNAPQRQPGGGSRPPQAGGLGNLASAAGGGLTRLILIGVVVIL
                     IVCAAFTVLLVTLNNLSNPAATPPPAGTPGTEGAAGGAGNGTVTIVGLNLGQVVMSSA
                     IDSANRPTAVTDTFATTTPAIYAVVPGVNVPAGSSLFARWLRDGQLFEDSSAITAPQA
                     YTNTFIQFTLQPKAGQTLPPGAYTVQIFGNGVQRSAAQFTVR"
     gene            1777..2241
                     /locus_tag="VF276_06615"
     CDS             1777..2241
                     /locus_tag="VF276_06615"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6606572.1"
                     /translation="MGYVENTVLVGRPRPAVFDAATTARYWPQWHPATRGVEGAIDHP
                     VQPGEAITEYVNIAGREGVAHWTCVQYQRPDRLVLDATGEGGTTARITYTFVGQDGGT
                     TFTRAMSYSFAGLPDGSPQAAALDQVMQTQSTVAMNNLKALIEAEVPAPDAG"
BASE COUNT          396 a          796 c          857 g          350 t
ORIGIN      
        1 gcgggcggcc tgcacctgcg cggccatctc ctgcggcgtc caaccgtagg catgccagga
       61 gaggaagcca acatcgccgc cgtcgtcctg cgccgtctca tagcccagca gccagtcgag
      121 cgccgtttcc tcgtacagcg acagagacgg cccgccgacc acggcgcgtg gatcgaggtc
      181 gtggatggcc cggcggctga tgtcgtagag gtggagatac tggggatagg acccctgcca
      241 ggcgccccac aggttcggct cgttccagat ctcccagtag cgcaggccca gcgcgcggtc
      301 gacgttgaaa tggcgcgcgg tagtatgcac cagcttgttc cactcctccc aatcgagcgg
      361 cggcagcaat tgcgattggg ggttgagggc gggcggcata tagctgaggt cgagcatcgg
      421 ctcggcgccc atggcccgaa tgtcgtccac cagcgtgtcg aggggcgtcc aatcgtaggt
      481 cagcccgccg ccgggccgcc gcgcgaccac gccggggtgg ttatagatgt ggtcgagccg
      541 cacatagcgg atgcccaggg ctttgatcag cgggattgtg gggtggagaa aggtggggtc
      601 gaacgactcg ccgccctggg ccagcccgat ctcgatgtgc tgggtgcgga ccggctccgg
      661 ctgcgccgcg acggtgacga ccggccagac cggggtcgcc gtgggcggcg gcccgcctcc
      721 cagcgccgag caggctgtga gcagcacgag caggccgaga gccgccggtc gcaggcgcgc
      781 gagcttagac ggcaggcgtg ataacgaacg taaggtcaac agagcacaga accagaccag
      841 gattgcgtgg cagccgcggg tgcgggggcg gcgaggcggc gggagcggca cagcatgtcg
      901 tcaccgcacc gtgaactggg ccgcgctgcg ttgcacgccg ttgccgaata tctgcacggt
      961 gtaggccccc ggcggcaagg tttgcccggc tttgggctgc aaggtgaact ggatgaaggt
     1021 gttggtatag gcttgcggcg cggtgatggc cgaactgtcc tcgaaaagct ggccatcgcg
     1081 caaccagcgg gcaaacaggc tgctgcccgc cggcacgttg acgccgggca ccaccgcgta
     1141 gatggccggg gtggtcgtgg cgaaggtatc ggtcactgcc gtcgggcgat tggcgctgtc
     1201 gatggcgctg ctcatgacca cctggcccag gttcaagccg acgatggtga cggtgccgtt
     1261 gccggcgccg cccgccgcgc cctcggtgcc cggcgtgccc gccgggggcg gggtggccgc
     1321 cgggttcgac aggttgttca gggtcaccag cagcacggta aaggcggcgc aaacaatgag
     1381 gatgacgacc acaccgataa ggattaaccg cgtgagtccc ccaccggcgg cgctcgccag
     1441 gttgccaagt ccacccgcct gcggcgggcg cgaccccccg ccgggttgcc gttgcggcgc
     1501 gttactcatc cctactcctc atctgcgaca tcccggcggc cctggactcc gtccatctag
     1561 ggcgccgccc caaccggcag gattgcgacc ggcggggaac ccttccacac cacgtgttgc
     1621 ggtgccatca tacacgcttt cggcggctcc cgcaattcgc gcgaccacag aacagggcct
     1681 gaaggcccgt ttcattatac ccggtccatc gcggaggatg ctataatggc accgtgcgcg
     1741 gcagcgtacc gggccggcgc acaggaggta gcgcagatgg gctatgtaga gaacaccgtc
     1801 ctggttgggc gcccgcgccc ggccgtgttc gacgcggcca ccacggcgcg gtactggccg
     1861 caatggcacc cggccacacg gggcgtcgag ggcgccattg accacccggt gcaacccggc
     1921 gaagcgatca ccgagtatgt gaatatcgcc gggcgcgagg gcgtggccca ctggacctgt
     1981 gtgcagtatc agcgccccga tcggctggtg ctggacgcga ccggcgaggg cggcaccacc
     2041 gcccgcatca cctatacctt cgtcggccag gacggcggca ccaccttcac gcgggctatg
     2101 agctacagtt ttgcgggctt gcccgacggc agcccccagg cggcggcgct ggaccaggtg
     2161 atgcagacgc aatcgacggt cgccatgaac aacctgaagg cgctcatcga agccgaagtc
     2221 ccggcgccgg acgccggata gaggaccacc atgccgggac agaactttga ccgggccgcc
     2281 gggatctacg acgcgacccg cggctggccg ccgggcgtcg ccgccgagat cggcgcgggc
     2341 ttgtaccagg ccctggcgcc cttcgcccag ccggggcagc ggctgcgggt gatcgaggc
//
LOCUS       DASUYC010000401         5733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1170992, whole genome shotgun sequence.
ACCESSION   DASUYC010000401 DASUYC010000000
VERSION     DASUYC010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5733
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1170992"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..154)
                     /locus_tag="VF276_06620"
     CDS             complement(<1..154)
                     /locus_tag="VF276_06620"
                     /inference="COORDINATES: protein motif:HMM:NF037411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606573.1"
                     /translation="MKIILSRKGFDSSIGKVPSPIFPSGELRSLPIPDHDQPPHAGGR
                     SYKDIRA"
     gene            complement(293..1456)
                     /locus_tag="VF276_06625"
     CDS             complement(293..1456)
                     /locus_tag="VF276_06625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6606574.1"
                     /translation="MKIAMMGIRGIPANYGGFETCVEAVATRLAARSHAVTVYNRPHH
                     VRWPASSYHGVHLVHLPTIANKYLDTLVHTTLAALHASAARADIVCVFVAANSPVTVI
                     PRLAGRKTVLHVDGLDWKRDKWPPAAKRYIRAAEWLGTRLPHTFITDSRVVQAYYRDT
                     FGAHAEYIAYGAEVEPMPPGPALAQFGLRPQEYILFVGRLVPENHIDVLIRAYQQVHT
                     DKKCVIVGHAPYADEYIGQLQALGGPNVIFTGYQFGAAYRELSSHAYLFVEPSTVGGT
                     HPALVEAMGFGNCVVAAGTAENRETIGEAGYSYGAEAPVANLGALLQRLVDAPAEVAA
                     ARARAAEHARVTFSWEQVTDAYEALFHRMLASGFVPRGRVRRLPVSHPDFRED"
     gene            complement(2119..3090)
                     /locus_tag="VF276_06630"
     CDS             complement(2119..3090)
                     /locus_tag="VF276_06630"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6606575.1"
                     /translation="MSDPAPRATEAAVLARLRCPACGGPLRESLRRGGEGRATCVDCS
                     AAYTQAGGIWRLLTEAERRRYAPFLESYRPLRQGDGWERADPAYYLRLPQVPPSDPTA
                     GIWRIRRRSLARLEQIAGPGAGRWALDLGAGNGWLSRRLTERGFAVIALDLNVGGADS
                     LEGAQVFLDHGGWFGRVQASMDSLPLAAGAFALCVCSGALHYADLPATLAEIWRVLEP
                     GGLFVCTDSPVYTDPAAGAAMVVEQAARLRANSGRDPQWPGGSGFLVADSLVDVMRRT
                     GFAVQMLANEHPLGPLRHRLAALRRPAAREQARFPVVVGRKAAPGGA"
     gene            complement(3087..3989)
                     /locus_tag="VF276_06635"
     CDS             complement(3087..3989)
                     /locus_tag="VF276_06635"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6606576.1"
                     /translation="MSADATAPLSDSESRTATAVYNAQSGQSFGRAAALFDSDEAANP
                     LARWARRRSFAALDAAFGPDDLVLEMGSGTGIEATHLAARGVRIVATDPATGMLEQLR
                     AKLAPGGPAAHLAGQITTLQLPANRIGDLVDRYGPGEFDGAYSSMGALNCEPSLALVA
                     AALATLVRPGGRLVFSLINRYCLWETAWYLRARQPGMAFRRWGGQASATVRPDWQEER
                     ITCFYWTPGAVARTFQPHFRVVGRRALPWLLPPLYLDGLTRRAPRFFRRVARLDDRFA
                     ARWPLYALGDQFIFELVREPVPAA"
     gene            complement(4082..5311)
                     /locus_tag="VF276_06640"
     CDS             complement(4082..5311)
                     /locus_tag="VF276_06640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6606577.1"
                     /translation="MSTRPPAPLKIALVVPGFAADAADWCIPALTNLARGLAARPGVD
                     LHVYALRYPHRQATYRLRGATVHAFGGAPLAGRRVWGASLGLLWTTFLAALRREHRRA
                     PFALLHGFWATESGYLTAVAGRMLGIPALVHLAGGELVHDLATGYGNQAPGLARFLVA
                     GSLRLATAITVPSGTQRALLHRRYPAHTGKAVDWALGVDIAMFAPGVATPTSAPGANG
                     PLRLVHAASLLPVKHQALLLDGLAAARTLAPDRPVALTVVGQGPLERSLRAQAARSGL
                     GDIVTFAGNQDHAALPALYRAHDAFVLTSRHEAQCMAVLEAAACGLPWIGPPVGALAD
                     LAAAIPTNGWSVPPDDALALGQAILAAGYPTGRIRRGAAARAAVERDYALDRQIDRLL
                     DLYAGALGRASRPHSHP"
     gene            complement(5308..>5733)
                     /locus_tag="VF276_06645"
     CDS             complement(5308..>5733)
                     /locus_tag="VF276_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606578.1"
                     /translation="VGWMVAQVGGLALYYRDPPHGADGARELAAVLRQDARPGDLVLV
                     TPPALMPTVRQYYAGPLRGLPSDFDLRAVYLPYDPVRWNADSLAVTTAALPGHPRAWL
                     VYRPELDAGGSFLATLQARYRQVDHRAYPFADLYLFTIP"
BASE COUNT          916 a         1993 c         2086 g          738 t
ORIGIN      
        1 cggcacggat atccttgtag gaccgcccac cggcgtgcgg cggctgatca tgatcgggta
       61 tcggtagcga acgaagctca cccgagggga aaatggggct gggcaccttg ccgatactgg
      121 agtcgaagcc ttttctgctc agaataatct tcatggcggc aatgacaccg ggcgcccggc
      181 gcggcgatcc tcgctaggtg gaaccggatc gcggcgggcg gccccggcgg ccggtacgac
      241 caatcgcgct agcgggtcgt caccatccgg tcctggccgc ccgccagggc gcctagtctt
      301 cgcggaagtc ggggtgcgag acgggcagcc ggcgcacccg gccccgcggc acaaaacccg
      361 atgccagcat ccggtggaag agggcctcgt aagcgtcggt gacttgctcc cacgagaagg
      421 tcactcgggc gtgctccgcc gcccgtgccc gcgccgccgc cacctccgcc ggggcgtcca
      481 ctaaccgttg cagcagcgcc cccaggttcg ccactggggc ctctgccccg tagctgtacc
      541 ccgcctctcc gatcgtctcc cggttctccg ccgtccccgc cgccaccacg cagttcccaa
      601 atcccatcgc ctccaccaac gccgggtgtg tgccccccac cgtcgacggc tccacgaaca
      661 ggtacgcgtg cgaactcaac tcccggtacg ccgccccaaa ctggtagccc gtgaagatca
      721 cgtttggccc ccccagcgcc tgcaactgcc cgatgtactc gtcggcatac ggcgcgtgcc
      781 ccacgatcac acatttcttg tcggtgtgta cctgctggta cgcccggatc agcacgtcga
      841 tgtggttctc cggcaccagc cgccccacga acaggatata ctcctgcggg cgcagcccga
      901 actgcgccag cgccggcccc ggcggcatcg gctcgacctc ggccccgtag gcgatgtact
      961 cggcgtgcgc cccgaacgtg tcccgatagt acgcctggac cacgcgcgag tcagtgatga
     1021 aggtgtgggg caggcgggtg ccgagccact cggcggcgcg gatgtagcgc ttggcggcgg
     1081 ggggccactt gtcgcgtttc cagtcgaggc cgtccacatg gagcacggtt ttgcgcccgg
     1141 cgaggcgcgg gatgaccgtg acgggggagt tggcggcgac gaagacgcag acgatgtcgg
     1201 cgcgggcggc gctggcatgg agggcggcga gggtggtatg gacgagggta tcgaggtact
     1261 tattggcgat ggtgggcagg tggacgaggt ggacgccgtg gtaggaggag gcgggccagc
     1321 ggacgtggtg ggggcggttg tagacggtga cggcgtggct gcgggcggcg aggcgggtgg
     1381 cgacggcctc gacgcaggtt tcaaagccgc cgtagttggc cgggatgccc cggatgccca
     1441 tcatggcgat tttcatactc cctcctcctt cataccggcc aggtcgcggg cggtaaggac
     1501 tggtgtgccc cccgcatgca ccaggccgcc gggcgcagac gccggccttc agtggtcata
     1561 gtgaaccggg cagggttcgg atgcagccgc cgggcgccgg tggtctcata tcgggccaga
     1621 tcaccggaca ggctaattca cagatgggct gcgatctcct gggcaagacg cggtactccc
     1681 ggttggcgcc aagtatcctt tcccaagtcg ggtcttcgcg ccgagcgcaa tcggctgcga
     1741 ccggctactg cccgcatcgc cgccggatgc cccggcgcgg cacatcccgt ggacactgca
     1801 cctgcagcgg tgctcttacg gcgccattgc cgccaggacg aggactcgtt gcagaatagc
     1861 aaccaaactg acgaacacac aaaagttaat tccggtagaa atcgcatttg cctgactacc
     1921 ggcagaccac cggccattat accacacggg acaaccggat gtcaggttcg cggtgctttc
     1981 gtccaaaata cgacaaagag agtttgctgc gtcaagccgg ccggtatggc cgatggacct
     2041 aaaacacctt caactacaac gccgatccgg cccggaaagt cacgggccac gcgcgccggt
     2101 tccccgcgga cctggggatc atgccccacc cggcgcggct ttgcgcccga cgaccacggg
     2161 aaagcgcgct tgctcgcggg ccgcgggccg gcgcagggcg gccagccggt ggcgcaacgg
     2221 gccgagcgga tgctcgttgg ccagcatctg gacggcaaag ccggtccggc gcatgacgtc
     2281 caccagcgag tccgccacca ggaacccgct gccgcccggc cactggggat cgcgcccgga
     2341 attggcccgc aaccgggccg cttgctcgac gaccatggcc gcacccgccg ccggatcggt
     2401 gtagaccggg ctgtcggtgc agacgaagag gccacccggc tccagcaccc gccagatctc
     2461 ggccagggtg gccggcaaat cggcgtagtg caaggcgccg ctgcacacgc agagggcgaa
     2521 cgcgcccgcc gcgaggggca agctgtccat cgaagcctgc acccggccaa accagccgcc
     2581 atggtccaga aacacctgcg cgccttccag gctgtcggcg ccgcccacgt tcaggtccag
     2641 ggcgatgacg gcaaagccgc gctcggtcag ccggcggctg agccagccgt tgcctgcgcc
     2701 caggtccagc gcccagcgcc cggcacccgg cccggcgatc tgctccagcc gggccaggct
     2761 gcgccgccgg atgcgccaga tccccgcggt gggatcgctc gggggcacct ggggcaggcg
     2821 caagtagtac gcgggatcgg cccgctccca gccgtcgccc tggcgcagcg ggcggtagga
     2881 ttcgaggaag ggtgcgtagc ggcggcgctc ggcctcggtc agcaagcgcc agatgccgcc
     2941 ggcctgggta taggcggcgc tgcaatcgac gcaggtggcc cggccctcgc cgccccgccg
     3001 cagggactcg cgcagcggac cgccgcaggc cgggcagcgc agccgggcca gcaccgccgc
     3061 ctcggtcgcg cggggcgcgg gatcactcac gcggcgggaa caggctcgcg gaccagctcg
     3121 aagatgaact ggtcgcccag ggcatacagc ggccagcggg cggcgaagcg gtcgtcaagc
     3181 cgggccacgc ggcggaagaa gcgcggggcg cggcgggtca ggccgtcgag gtagagcggt
     3241 ggcaggagcc agggcagcgc ccgccggccc acgacgcgga aatgcggctg gaaggtgcgc
     3301 gcgacggcgc cgggggtcca gtaaaagcag gtgatacgct cttcctgcca atcggggcgc
     3361 acggtggccg aggcttgccc gccccagcgg cggaaggcca tgcccggctg ccgcgcccgc
     3421 aagtaccagg ccgtttccca caggcagtag cgattgatca gcgagaagac cagccgcccg
     3481 ccggggcgga ccagggtggc gagcgccgcc gccacgagcg ccagcgacgg ctcgcagttc
     3541 agcgcgccca tgctcgaata cgcgccgtcg aactcgcccg gcccgtagcg atccaccagg
     3601 tccccgatgc ggttggcggg caattggagc gtcgtgatct gcccggccag gtgcgcggcg
     3661 ggaccgcccg gcgccagctt ggcgcgcaac tgttcgagca tgccggtggc cgggtcggtg
     3721 gcgacgatgc gcacgccgcg cgccgccagg tgggtggcct cgatgccggt gcccgacccc
     3781 atttccagca ccaggtcgtc ggggccgaag gcggcgtcga gcgcggcgaa gctgcggcgg
     3841 cgcgcccagc gggccagcgg gttcgcggcc tcgtcgctgt cgaacagggc cgccgcccga
     3901 ccaaaggatt gccccgactg ggcattgtag acggcggtcg cggtgcgcga ttcggaatcg
     3961 gacaggggag cggtggcgtc cgcactcata aacagggttc cattccagac ggctaggcgc
     4021 gagggcgccg gcatagggtt gcggtagctg ggcctatgat agcagcgccc cgccgccgct
     4081 gtcaaggatg ggagtgcggg cgagaggcgc ggcccagggc gcccgcataa aggtccagca
     4141 agcggtcgat ctgccgatcc agggcgtagt cgcgttcgac ggcggcgcgg gcggcggcgc
     4201 cccggcggat gcgcccggtc gggtagccgg cggccaggat ggcctgcccc agcgcgaggg
     4261 cgtcgtcggg cgggactgac cagccgttcg tggggatggc cgcggccagg tcggccagcg
     4321 ccccgaccgg gggaccgatc cagggcagcc cgcaggccgc cgcttcgagc acggccatgc
     4381 actgcgcctc gtggcgcgag gtcagcacga aggcgtcgtg ggcacggtag agcgcgggca
     4441 gcgcggcgtg gtcctggttg ccggcgaagg tgacgatgtc acccaggccc gaccgggcgg
     4501 cctgggcccg caacgacctt tccagcgggc cctggcccac caccgtcagc gccacgggcc
     4561 gatccggcgc cagggtgcgc gctgccgcca gcccgtcgag cagcagcgcc tggtgcttga
     4621 ccggcaacag cgacgcggcg tggaccagac gcagcgggcc gttggccccc ggcgccgatg
     4681 tgggtgtcgc gacgccgggc gcgaacatcg cgatatcgac gcccagcgcc caatcgacgg
     4741 ctttccctgt atgggcagga taacggcgat gcaacagcgc gcgctgggtg cccgacggca
     4801 cggtgatcgc cgtggcgagg cgcaggctgc ccgcgaccag gaagcgggcc aggccgggcg
     4861 cctggttgcc gtacccggtc gccaggtcat gcaccagttc gcccccggcc aggtggacca
     4921 gcgccgggat gccgagcatg cgcccggcta cggcggtcag gtagcccgat tcggtggccc
     4981 agaagccgtg cagcagagcg aagggcgcgc gccggtgttc gcggcgcagg gcggccagga
     5041 acgtggtcca gagcaggccc aggctggcgc cccacacccg ccgcccggcc agcggtgccc
     5101 cgccgaaggc atggaccgtg gccccgcgca ggcgataggt cgcctgccgg tgggggtagc
     5161 gcagggcata gacatgcagg tcgacgccgg gccgcgcggc caacccccgc gccaggttgg
     5221 tcagcgcggg gatgcaccaa tcggcggcat cggcggcgaa gccgggcacc accagcgcga
     5281 ttttcagcgg ggcgggcgga cgtgtgctca tgggatggtg aacaggtaga gatcggcgaa
     5341 ggggtaggct cgatgatcca cctgccggta gcgcgcctgc aacgtcgcca ggaaactacc
     5401 gcccgcgtcg agttcggggc ggtagaccag ccacgcccgc gggtggccgg gcagcgccgc
     5461 cgtggtcacg gccagggaat cggcgttcca gcgaaccgga tcatagggca ggtagaccgc
     5521 gcgcagatcg aaatcgctcg gcagcccgcg cagcggcccg gcgtaatact gccgcaccgt
     5581 cggcatgagc gcgggcggcg tgaccagcac gagatcgccg ggccgggcat cctggcgcaa
     5641 cacggcggcc aattcccgcg ccccgtcggc cccatgcggc ggatcgcgat aatagagcgc
     5701 caaccccccc acctgcgcca ccatccaccc cac
//
LOCUS       DASUYC010000402         2826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1431776, whole genome shotgun sequence.
ACCESSION   DASUYC010000402 DASUYC010000000
VERSION     DASUYC010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1431776"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..566
                     /locus_tag="VF276_06650"
     CDS             270..566
                     /locus_tag="VF276_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606579.1"
                     /translation="MAGESKTTTDHKEIRKWVEARGGSPATVKRTGNDDDPGVLRIDF
                     PGYSGGDSLEHISWDAFFEKFDEKHLAFLYQDKTKDGEESRFFKLVSQDTAHKK"
     gene            763..1521
                     /locus_tag="VF276_06655"
     CDS             763..1521
                     /locus_tag="VF276_06655"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX6606580.1"
                     /translation="MRIAVISDMHGNCLALDAVLADLRRDPVDQVVCLGDAIQGGPQP
                     AEVVARLRELACPVVMGNADDWLLTGQDPTTQEEVSERQRRVREWSLAQLPADDQAFI
                     AGFQPTVEISLGPDTDRTLICFHGSPHSYNDVILPETPEAEFAQMLGPFQPAILTGGH
                     THMQQVRRLGDTFFFNPGSVGFAYNRHQPEEDFKADPWADYAVLGVDQGRISLEFRRV
                     PFDAAEMVRIILASGVPYAEDQVARYEPRDPTGP"
     gene            1536..2507
                     /locus_tag="VF276_06660"
     CDS             1536..2507
                     /locus_tag="VF276_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606581.1"
                     /translation="MPDKIIARILRESGVANLVELLGEDLAPTDLQSLLLAVFHRRAA
                     RQTPRRVLDQYAQNPFVRPAPVDAGALLELDRLALRAAAPAFAALDLSPLAPLGTCSA
                     LAPVDQNRIVSTIRNTEVVADATNVLALECALRRRAQRHDSEPVREPVRVCASHRLVR
                     AQRYTGPHMRAHFRMFSLCTAGRAAGLGGFEVPALNEQLGFYLHFLAGAEELGYHLAD
                     TRIAFTPLADEAARDILAQQVIAPVAAQFPRARLEFDPHPTTTPGYYQWVRFQIYARD
                     LQGTEYMIGDGGFTDWTRQLLSDRHERLLTSGIATERIATLFRAGPP"
     gene            complement(2527..>2826)
                     /locus_tag="VF276_06665"
     CDS             complement(2527..>2826)
                     /locus_tag="VF276_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX6606582.1"
                     /translation="TERTREIGIRKAVGAQQGDILRQFLIEAVLISLSGGLMGIALGM
                     GLASLVSLTGTLTPVVTPGAALLATGFSVAVGLFFGIYPARRAARLDPIVALRYE"
BASE COUNT          478 a          958 c          959 g          431 t
ORIGIN      
        1 cgaagagtct cgtctttcta gctacgagac ggagattctt cgctccgctc agaatgacaa
       61 tttgagtgtg acagcctact gagcggagcg aagagtctcg tcgccacgga gacgagatgc
      121 ttcactgcgt tcagcatgac aatgttgaca ggaagcagga ttgggccggg ccaccggggc
      181 tggcacggga gttgcaccaa cataccctgt tacaacccgg cgggccgcca aggccaggca
      241 gtctgcccac gcagccatgg aggtgcagta tggcaggaga atcgaagaca acgactgatc
      301 acaaggagat ccgtaagtgg gtcgaggcgc ggggcggctc gcccgccacc gtgaaacgca
      361 ccggcaacga cgacgacccc ggcgtgctgc ggatcgattt ccccggctac agcggcggcg
      421 actccctgga gcacatctcc tgggatgcgt tctttgagaa gttcgacgag aagcacctgg
      481 ccttcctcta ccaggacaag accaaagacg gcgaggaaag ccggttcttc aagctggtga
      541 gccaggatac cgcccataag aagtagccgg aacaccccag gcaagaccgg gcgcgcgacg
      601 cccggtcttc cttttgcccg gcgaccagga cttgtcacgt ggagcgcagc gcggaatcga
      661 tactttgtgg atcaagcctc ataactactg tgttgctacc gggggcgcgc ctgggccacc
      721 ggcatgcttc ttgcggcctc taccgtgagg agggtggtac caatgcgaat cgcggttatc
      781 tcggatatgc acggcaactg cctggcgctc gatgctgtgc tggccgacct gcgccgggac
      841 ccggtggatc aggtcgtctg cctgggcgat gcgatccagg gcgggccgca gcccgccgaa
      901 gtcgtggccc ggctgcgcga actggcctgc ccggtcgtca tgggcaacgc cgacgactgg
      961 ctgctgaccg gccaggaccc cacgacgcag gaagaagtct ctgagcggca gcgccgggtt
     1021 cgcgagtggt cgctggcgca actgccggcg gacgaccagg cgttcatcgc cgggttccag
     1081 cccacggtgg aaatctcgct ggggccggac acggaccgca ccttgatctg cttccacggc
     1141 tcgccccatt cctacaacga cgtgattctg ccggagacgc ccgaagccga gttcgcgcag
     1201 atgctcggcc cgttccagcc ggcgatcctg accggcggcc atacgcacat gcaacaggtg
     1261 cgccgcctgg gcgacacgtt cttcttcaac cccggcagcg tcggcttcgc ctacaaccgc
     1321 catcaacccg aagaggactt caaggccgac ccgtgggccg actacgccgt gctgggcgtg
     1381 gaccaggggc gcatcagcct ggagttccgc cgcgtaccct tcgacgccgc cgagatggtg
     1441 cgcatcatcc tggcgagcgg cgttccctat gccgaggacc aggtcgcccg ctacgagccc
     1501 cgcgacccga ccgggccgta agcaggtcgc ctgccatgcc ggacaagatc atcgcacgca
     1561 tcctgcgcga gagcggggtc gccaacctgg tcgagttgct gggcgaggac ctggcgccga
     1621 cggatctgca atcgctgcta ctggccgtct tccaccggcg ggccgcgcgc cagaccccgc
     1681 gccgcgtgct ggaccagtac gcgcaaaacc cgtttgtgcg cccggcaccg gtggatgccg
     1741 gcgcgctact ggagttggac cggctggcgc tgcgcgcggc ggcgccagcc ttcgcggcgc
     1801 tcgatctgtc gcccctggcg ccgctgggca cctgctcggc gctggcgccg gtggaccaga
     1861 accggatcgt cagcaccatc cgcaacaccg aggtggtggc cgacgcgacg aacgtgctgg
     1921 cgctggaatg cgcgctacgg cggcgggcgc aacggcatga tagcgagccg gtgcgggaac
     1981 cggtgcgggt ctgcgccagt caccgcctgg tgcgggcgca acgctacacg gggccgcata
     2041 tgcgcgccca cttccgcatg ttcagcctct gcaccgccgg gcgggcggcg ggattgggcg
     2101 gctttgaagt gcccgcgctc aacgagcagc ttggcttcta tctgcacttc ctggcgggcg
     2161 cggaggagtt agggtatcac ctggcggaca cgcgcatcgc cttcacgccg ctggccgatg
     2221 aggcggcgcg cgacatcctc gcgcagcagg tgatcgcccc ggtcgccgcc cagtttccgc
     2281 gggcgcgcct ggagttcgat ccccatccga ccacgacgcc ggggtattac cagtgggtgc
     2341 gcttccagat ctacgcccgc gatctgcagg gcacggaata catgatcggc gacggcgggt
     2401 tcaccgactg gacccggcag ttgctcagcg accggcacga gcggctgctg accagcggca
     2461 tcgccaccga gcgcatcgcc accctgttcc gcgccgggcc gccgtaaccc cgcccggcgg
     2521 gtgccgctat tcgtagcgca gggccacgat ggggtcgaga cgcgccgcgc ggcgggccgg
     2581 gtagatgccg aagaacagcc ccaccgccac cgagaagccg gtcgccagca gcgccgcgcc
     2641 gggcgtgacc acgggcgtga gcgtgccggt gaggctgacc agggaggcca gccccatgcc
     2701 gagcgcgatg cccatcagcc cgccgctcag gctgatcagc acggcctcga tcaggaactg
     2761 gcgcaggatg tcgccctgct gggcgccgac ggctttgcgg atgccgatct cgcgggtgcg
     2821 ttcggt
//
LOCUS       DASUYC010000403         2344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_529712,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000403 DASUYC010000000
VERSION     DASUYC010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529712"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(758..>2344)
                     /locus_tag="VF276_06670"
     CDS             complement(758..>2344)
                     /locus_tag="VF276_06670"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX6606583.1"
                     /translation="AVEQLERAREYASEAHNVRLQAGVLSNLASVQQVQGDLRLAESN
                     MRAALDIARSLHWRRLEAETLLRLADNTLELTGPPAAHPLYEEASEVGRRAAPAVVVG
                     ALAGQARCARRLGRYPEAIRLAQQGLDTARAGEMPFETALCRLELGAATLVHSPEDAL
                     PLLRDAEQTLVGLGRKREATRAAAILATVLFATGDFAGADAALDRAATAGEARGTTPY
                     LTPELATIHGLPLLRRAGGEEGRYAALFAAVVRYLEAEHEVEPGGPGESGIQIVRGRP
                     RLLEVYGLGQASVYCDGVLLARPDWQSATAKELFFYFIEHPEGLRKEVIQAALWPDQT
                     LSRANDNFHTSLKRLRKAVGMEIVKIEEAVYRLNPTLAIWHDGAEALALIEQAKATPD
                     LDEARRAFTAAAALLKGPFAEEFYRDWAGARRQFWQTRTREALMWLADDAVRTGDFTA
                     AVTWGQRLLVIDPLDESAHGLLMHIYAAAGSPAKLSQQYHELCRILQYELGGTPSPEI
                     TALYKRLLNADPGQARFPAR"
BASE COUNT          354 a          763 c          840 g          387 t
ORIGIN      
        1 cgctattcta ccaggggggt tctgacgaac ctctgacaaa ctcggcgatt tgcgcgccgg
       61 atttcgtcaa caccgctatt ctaccagggg ggttctgacg aacctctgac aaaccgcacc
      121 ggaatcgcgc gaattttgcc ccaatttgga gccggaggca cgattgggcg attttcgcga
      181 gctggtgcga gcgacggcgc gggttggagt gatcctccct cggatcggcc gggcgcagcc
      241 gcggacgggt tcgctgggtc gccccatccg tggtgcatcg ggcgcagccg atgcccgccc
      301 tcgccacaag ggtggggcat cggtatgccg gtatgctcca atgtgcaacg aatggtgaat
      361 cgcaggtgtg gtgtgcgaca agttgtgctt atggatatga cgaacaagtc gcgccgagga
      421 ctcgcaaatg gggctagcaa acggaatctc gtaggggttg gttcgcaaac cggccccccg
      481 tggattgcgg acggcgcttc ccccacgccg ttacggcgta ggggcgggtt tcaacccagc
      541 gcggtccacc acggtggttc ggcgtagggg ccggtttgaa accggcccgc acgcggcccg
      601 cggcaccgat ttggttgccc aggggccggg gaggtggggc ggcaggcggg tttgaaaccg
      661 gcccctacac cggcggggac ggcgggcgtg ccgccgggcg aaacacgccg gtcggcccgg
      721 cctgcgtctc tcgccagcac ggcgccaaca gcactcgtca gcgggcgggg aaccgggctt
      781 gccccggatc ggcgttgagc aggcgcttat agagcgcggt gatttcgggg ctgggcgtgc
      841 cgccgagctc gtactgcaaa atacggcaga gttcgtggta ttgctggctg agcttggccg
      901 ggctgcccgc agcggcatag atatgcatca gcaatccatg cgccgattcg tccagggggt
      961 cgatgaccag cagccgctgg ccccaggtca cggcagcggt gaaatcgccg gtgcgcacgg
     1021 cgtcgtcggc cagccacatc agcgcctcgc gggtacgggt ctgccagaac tgacggcggg
     1081 cgcccgccca gtcgcggtag aactcttcgg caaacggccc cttcagcagg gcggcggcgg
     1141 cggtgaaggc gcggcgggcc tcgtcgagat cgggtgtggc cttggcctgc tcgatcaggg
     1201 ccagggcttc cgcgccgtcg tgccagatag ccagcgtggg attgagccgg tagacggctt
     1261 cttcgatctt gacgatttcc atgccgacgg ctttgcgcag ccgtttcaac gaggtgtgga
     1321 aattatcgtt ggcgcggctc agggtctggt ccggccagag cgcggcctgg atgacctctt
     1381 tgcgcaggcc ctcggggtgc tcgatgaaat agaagaacag ttccttggcg gtggcgctct
     1441 gccagtcggg gcgtgccagc agcaccccgt cgcaatagac gctggcctgc ccgagaccgt
     1501 agacttcgag caggcggggc cgcccgcgca cgatctggat gccgctctcg cccggcccgc
     1561 ccggctccac ttcgtgttcg gcctccagat agcggacgac ggcggcaaac agggcggcat
     1621 agcgcccttc ctcgcctccg gcgcggcgca acagcggcaa gccgtggatc gtggcgagtt
     1681 cgggcgtcag atacggcgtg gtgccgcggg cttcgcccgc cgtggccgcg cggtcgagcg
     1741 ccgcgtcggc gcccgcgaag tcgcccgtgg caaacaggac cgtcgccagg atcgcggcgg
     1801 cgcgcgtggc ttcgcgctta cggccgaggc cgaccagggt ttgctcggcg tcgcggagca
     1861 gcggcaaggc gtcttcgggc gagtggacca gggtggccgc gccgagttcc agacggcaca
     1921 gggccgtttc aaagggcatt tcgccggcgc gggccgtgtc cagcccttgc tgggccagcc
     1981 ggatcgcctc cgggtagcgg cccaggcggc gcgcgcagcg ggcctgcccg gcgagcgcgc
     2041 ccaccacgac ggcgggagcc gcgcgccggc ccacctcgct cgcctcttcg tacagcggat
     2101 gggcggcggg cggcccggtc aattccagcg tattatcggc cagccgcagc agggtctcgg
     2161 cttccagccg ccgccagtgg agactgcgcg cgatgtccag cgcggcccgc atattgcttt
     2221 ccgccagccg cagatccccc tgcacttgtt gcaccgaggc caaattggac aggactccgg
     2281 cttgcaaacg gacgttgtgc gcctcggacg catattcgcg cgctctttca agctgttcca
     2341 ccgc
//
LOCUS       DASUYC010000404         2280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_8377,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000404 DASUYC010000000
VERSION     DASUYC010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8377"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            255..1265
                     /gene="trpS"
                     /locus_tag="VF276_06675"
     CDS             255..1265
                     /gene="trpS"
                     /locus_tag="VF276_06675"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019588161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX6606584.1"
                     /translation="MKKRILTGDRPTGPLHLGHYVGSLANRVRLQHEYETYILIADVQ
                     ALTDNFDNPEKVSRSVREVALDYIGVGLDPNIATIVVQSLVPEIAELTVFYSNLVTVA
                     RLERNPTIKTELVERGMEENVTYGFLGYPVSQAADITFVKAHLVPVGDDQLPHIELTR
                     DIVARFNRTYGDVFPEPEGLISNFPRLPGTDNQGKMSKSLGNAIYLSDDAETVRKRVM
                     SMYTDPTRLRATDPGHVEGNPVFAYHDAFNPDTARVEEMKDLYRKGGIGDVQVKKELA
                     VALNTFLDPIRERRNAAAQHPEQIDEMLIEGTRRARAIARETMRDVRNAMQINYFPGV
                     EL"
     gene            complement(1405..2238)
                     /locus_tag="VF276_06680"
     CDS             complement(1405..2238)
                     /locus_tag="VF276_06680"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6606585.1"
                     /translation="MEQRVVIVTGASSGIGWAVARWAAAKEFRVVLAARREDRLRDLA
                     ARIEEDGGTALVVPVDVAKPEDQRRLIDETVRGFGRIDVLVNNAGLPLPTPFAESPPE
                     DLRRQWDVNVTALATLTRLALPYLEQHRHELHPATVVNIGSGISRFAVPGMGNYSPTK
                     IAVAGLSEALRRELAPRGVQVCLVEPGPVATEFGARAGDKGVVPSRLVVPAAKAAKAI
                     VRLFDHPQRRIVIPARLGPVLTLAGGLAGAFPHLVDAVVRRSLRQGRFARYMEAGEQA
                     G"
BASE COUNT          390 a          814 c          746 g          330 t
ORIGIN      
        1 gacggtggaa acccggcatc ggcgcgggcg gggaatggat ctgggagtcg cgcaccaaac
       61 gggggtcgcc ccaagtaggc tgcgccgggc gcctccgccg ttacccggag tgagtgatcc
      121 gccgccgccg cggcgcgggc actcgtcacg agcgcgcgat ccggtggggg agcgcggaac
      181 tggggtggca ccacgagacg gctttggccc tcgtccctga ttggggacgg gggttttttg
      241 ttggagacaa aaccatgaag aagcgcattt taaccgggga ccggcccacc gggccgctcc
      301 acctgggcca ttatgtcggc tcgctggcaa accgcgtgcg gttgcagcac gagtacgaga
      361 cctacatcct gatcgccgat gtgcaggcgc tgaccgacaa cttcgacaac ccggagaagg
      421 tcagccggtc ggtgcgcgag gtggcactcg actatatcgg cgtgggactg gacccgaaca
      481 tcgccaccat cgtcgtgcag tcgctggtgc cggagattgc cgaattgacc gtgttctact
      541 cgaacctggt cacggtggca cggctggaac gcaacccgac gatcaagacc gagctggtcg
      601 agcgcggcat ggaagagaac gtgacctacg gcttcctggg ctacccggtg tcgcaggccg
      661 ccgatatcac cttcgtcaag gcgcacctgg tgccggtagg cgacgaccag ttgccgcaca
      721 tcgaactgac gcgcgacatc gtggcccgct tcaaccgcac ctacggcgac gtgttccccg
      781 agcccgaggg gctgatcagc aacttcccgc gcctgcccgg caccgacaac cagggcaaga
      841 tgagcaagag cctgggcaac gccatctacc tgtccgacga tgccgagacg gtgcgcaagc
      901 gcgtgatgag catgtacacc gacccgaccc gcctgcgggc caccgacccc ggccacgtcg
      961 agggcaatcc ggttttcgcc taccacgacg ccttcaaccc cgacacggcg cgggtcgagg
     1021 agatgaagga cctctaccgc aagggcggca tcggcgacgt gcaggtcaag aaagaactgg
     1081 cggtagcgct caacaccttc ctcgacccga tccgcgagcg gcgcaacgcg gcggcccagc
     1141 accccgagca gatcgacgag atgctgatcg agggcacgcg ccgcgcccgc gccatcgccc
     1201 gcgagacgat gcgcgacgtg cgcaacgcca tgcagattaa ctacttcccc ggcgtcgagc
     1261 tctagccgcc ggcatcccgg tgagggcgac aggcgcgatc aatcgcgccg ctacgaacga
     1321 ggcaaggtaa cgcaccttcg taggggcgcg attgatcgcg cctgctgcct taccccgatc
     1381 tccggggttc aggccagatc gccgctaccc cgcctgttcg ccggcctcca tgtaacgggc
     1441 gaagcggccc tggcgcaggc tgcgccgcac cacggcgtcc acgaggtgcg ggaacgcccc
     1501 ggccagcccc ccggccagtg tcagcaccgg gccgagccgc gccgggatga cgatgcgccg
     1561 ctgcgggtgg tcgaacagcc gcacgatggc cttggccgcc ttagccgcgg gcacgaccag
     1621 gcgcgacggc accaccccct tgtcgcccgc ccgcgccccg aactcggtgg cgacggggcc
     1681 gggttccacc aggcagactt gaacgccgcg cggcgccaac tcccgccgca gcgcctcgct
     1741 cagcccggcc acggcgatct tggtcgggct gtagttgccc atgcccggca cggcgaaccg
     1801 gctgatgccc gatccgatgt tgactacggt ggccgggtgc aattcgtggc ggtgctgctc
     1861 caggtagggc agggccagcc gcgtcagggt ggcgagggcc gtgacgttga catcccactg
     1921 ccggcgcagg tcctcgggcg gcgactcggc gaacggcgtg ggcaacggca gccccgcgtt
     1981 gttcaccagc acgtcgatcc gcccgaaccc gcgcaccgtc tcgtcgatca atcgccgctg
     2041 gtcctccggc ttggcaacat cgaccggcac caccagcgcc gtcccgccgt cttcctcgat
     2101 ccgcgcggcg aggtcgcgca ggcgatcttc gcgccgggcc gccagcacca cccggaactc
     2161 cttggccgcc gcccagcggg ccacggccca accgatcccg cttgacgccc cggtcacaat
     2221 cacaacacgc tgttccatag ttttggtacg cttccttctt gctgattctt tgcggcaccc
//
LOCUS       DASUYC010000405         3441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_280679,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000405 DASUYC010000000
VERSION     DASUYC010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280679"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..1557)
                     /locus_tag="VF276_06685"
     CDS             complement(22..1557)
                     /locus_tag="VF276_06685"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEX6606586.1"
                     /translation="MTDSDDAPPDLAVFAPVLEAARDGIVVVDDDYRYLYVNPAAARM
                     LGYPRAALIGRELLLNFPPRERPAIREQIAASLESPHAPAITTILRPDGEEREISYTS
                     TRVVIAGRSCGAAILRDVTEESRRVIREREALIKIASTVAIDGSLDDVLDGLAVKVVS
                     ATGALACAVLLLEGDPPRARVAGTSGLPPDFRASIEAAMRAGAELPSMRAAHERHPVI
                     VRDIRDFILAQPAFAPIHHIVRQATWDTLVSVPLTYRHRDVGILATYYAAGHYPDETQ
                     LAFLQAIAGQAAVAVENARLLIEAHGKAALEERQRLARDLHDSVSQALFGIALGARTA
                     RTLLQQDPRAAAEPLDYVLSLAEAGMAEMRALIFELRPESLQTEGLLAALNKEIAALR
                     ARYGITVDAVLGTEPDLPLEIKEALYRISQEALHNTVKHARATHVDLRLEQFPDRVAL
                     DIRDNGIGFDPTGHFPGHLGLRSMRERVSRLGGTLAVESAPEQGTHIRAEIPTNPPAP
                     ASE"
     gene            2055..2843
                     /locus_tag="VF276_06690"
     CDS             2055..2843
                     /locus_tag="VF276_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6606587.1"
                     /translation="MNMNGAGASFQGAAIAAQGLRKVYGAHEAVKHVDLLIKSGEIVG
                     FLGPNGAGKTTTIKMLTGLLKPTAGQSSIMGHDIQREPVAAKACFGYVPDTPNLYGKL
                     TGWEFLRFMSRLYRVPQAQAERRAGELLRLFELNTAASDLIEGYSHGMQQKMALAGAL
                     VHDPQVLFLDEPTVGLDPRSARLIKDLLGQLRDRGTAVFFSTHILEIAERMCDRVVII
                     NNGEIVASGTLAELRARQGEGSLEDIFLRLTGGAEYAEMAEVLA"
     gene            2840..>3441
                     /locus_tag="VF276_06695"
     CDS             2840..>3441
                     /locus_tag="VF276_06695"
                     /inference="COORDINATES: protein motif:HMM:NF028259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606588.1"
                     /translation="MIADTWLLLGLRWQTAWNGFRHGRAWRQALTILAMVAVAVGLGS
                     FSAAVGVLFGEILRHFPSANLDALLPGVLLTIVAVILLLSSFGLALGSLFLSSDLELL
                     MSAPVDRRAVFLSKILDGMGTNYGILAVTALPALVAYGIGIGYGPLYYLFAIIAVIGV
                     PLLPAALGALLVLVVARFAPARRVREVLGLMAALFGISCS"
BASE COUNT          511 a         1100 c         1224 g          606 t
ORIGIN      
        1 tttgcttttg ccgatccgcc gtcattcgct agcgggtgcg ggcgggttgg tggggatctc
       61 ggcgcgaata tgcgtgccct gttcgggggc gctctccacc gccagcgtgc cgcccaggcg
      121 gcttacccgt tcccgcatcg agcgcaggcc gagatggccg gggaaatggc cggtggggtc
      181 gaaaccgatg ccgttgtcgc gaatgtccag ggccacgcgg tcggggaact gttccaggcg
      241 caagtccaca tgcgtggcgc gggcgtgctt cacggtgttg tgcaacgcct cctggctgat
      301 gcggtagagc gcctccttga tctccagcgg cagatcgggc tccgtgccca gcaccgcatc
      361 gacggtgatg ccgtagcggg cgcgcaacgc cgcgatctcc ttgttcagcg cggcgagcag
      421 cccctcggtt tgcagcgact cggggcggag ttcgaagatc agcgcgcgca tctcggccat
      481 gccggcttcg gccagcgaca gcacgtagtc cagcggctcg gccgccgcgc gcgggtcctg
      541 ttgcagcagg gtgcgggcgg tgcgcgcccc gagggcgatg ccgaacaggg cctgcgacac
      601 cgagtcgtgc aggtcgcgcg ccagccgctg ccgttcttcg agcgccgctt taccgtgggc
      661 ctcgatcagc agccgcgcat tctccaccgc caccgccgcc tgcccggcga tggcttgcag
      721 aaaggctagc tgggtttcgt cgggatagtg gcccgccgca taataggtcg ccaggatgcc
      781 gacatcgcgg tggcggtagg tgaggggcac gctgaccagc gtgtcccagg tcgcctgccg
      841 cacgatgtgg tggatgggtg cgaaggcggg ctgcgcgagg atgaagtcgc ggatatcgcg
      901 gacgatgacc ggatgccgct cgtgcgccgc gcgcatactc ggcaactcgg cgcccgcgcg
      961 catggctgct tcgatgctgg cgcggaagtc gggcggcagg ccgctggtgc ccgcgacccg
     1021 cgcccgcggg ggatcgcctt ccagcagcag caccgcgcag gccagcgcgc cggtggcgct
     1081 caccaccttg accgccagtc catccagtac gtcgtccagc gagccgtcga tggcgacggt
     1141 cgaggcgatc ttgatcagcg cctcgcgttc gcggatcacg cggcggctct cctcggtcac
     1201 gtcgcgcagg atggccgcgc cgcaggatcg cccggcgatg accacacggg tgctggtata
     1261 gctgatctcc cgctcctcgc cgtcggggcg caggatcgtc gtgatggccg gggcgtgggg
     1321 gctttccagg ctggcggcga tctgttcccg gatggcgggg cgctcgcggg gcggaaaatt
     1381 gagtaacagt tcgcgcccga tgagagccgc gcgcgggtag cccagcatgc gcgccgccgc
     1441 cgggttgaca tacagatagc ggtaatcgtc gtccaccacg acaatgccgt cccgtgcggc
     1501 ttccagcacc ggggcgaaca cggccaggtc agggggcgcg tcgtccgagt cggtcatagg
     1561 cttcaagaga tgcggacgcg cggcggtgcg tccattggca tacatcggac ccgatcagcc
     1621 gggcgacggg aattcacctg gcggcccggc gcctcattcg cggcgatcct gagcatgatt
     1681 cgatcctcgc tcttgcgcca ggcgcgcccg ctcggcggct ctgggctaaa gacggtctcc
     1741 gatccaactt ccggcggatt gacctcgggc ggcgaactct gtatcattgt atcagttcgc
     1801 taagacaacc ggcgcgcccg catcgtgggg cgattctata cctttcgctg gtgaccatct
     1861 cctatcgaag gtccgacatg ccgggctgcc ctgggtagga gcccatgtaa ccccggcgtg
     1921 acgtataatg tatatagagg tgcgggaaaa agcgacccac cttataccag aaatcccctg
     1981 gcgcttcgac tccggatgta tgcattgccg ggattgagcg ccgatctgtt tgcattggag
     2041 aagggaagtt gttgatgaac atgaatggag cgggcgcgtc gttccagggc gcggccattg
     2101 ccgcccaggg cttgcggaaa gtctacggcg cgcacgaggc ggtcaagcac gtcgatctgc
     2161 tgatcaaatc gggcgaaatc gtcgggttcc tggggccgaa cggcgcgggc aaaacgacga
     2221 cgatcaagat gctgaccggg ttgttgaagc ccacggcggg ccagtcgagc atcatgggtc
     2281 acgatataca gcgcgagccg gtggcggcca aagcctgttt cggctacgtg cccgatacgc
     2341 ccaacctcta cggcaagctc accgggtggg agtttttgcg cttcatgagc cggctctacc
     2401 gcgtgccgca ggcccaggcc gagcgccgcg cgggcgaatt gctgcgcctg ttcgagttga
     2461 acacggcggc cagcgacctg atcgagggct acagccacgg catgcagcag aagatggccc
     2521 tggcgggcgc cctggtgcat gacccgcagg tgctctttct ggacgagccg accgtcggcc
     2581 tcgacccgcg ctcggcccgc ctgatcaagg acctgctggg ccagttgcgc gaccggggca
     2641 cggcggtctt cttctcgacc catatcctcg aaatcgccga gcgcatgtgc gaccgggtgg
     2701 tcatcatcaa caacggcgaa atcgtcgcct cgggcacgct ggccgagttg cgggcgcgcc
     2761 agggcgaggg gtcgctggaa gacatcttcc tgcggctgac cggcggagcc gagtacgccg
     2821 aaatggccga ggtcctggca tgatcgcgga tacctggctg ctgttggggc tgcgctggca
     2881 gacggcgtgg aacggcttcc gccacgggcg ggcctggcgc caggcgctga ccatcctggc
     2941 gatggtggcc gtggccgtgg gcctggggtc cttctcggcg gcggtgggcg tgctgttcgg
     3001 cgagatcctg cgccatttcc ccagcgccaa tctcgacgcg ctgctgccgg gtgtgttgct
     3061 gaccatcgtg gcggtgatcc tgttgctcag cagcttcggg ctggcgttgg ggtcgttgtt
     3121 cctgagcagc gacctcgaac tgctgatgag cgcgccggtg gaccggcggg ccgtcttcct
     3181 gagcaagatc ctggacggca tgggcaccaa ctacggcatc ctggccgtga ccgcgctacc
     3241 ggccctggtg gcctacggca tcggcatcgg ctatggcccg ctgtactacc tgttcgccat
     3301 aatcgccgtg atcggcgtgc cgctgctgcc cgcggcgctg ggcgcgctgc tggtgctggt
     3361 ggtggcgcgg ttcgcgcccg cgcgccgggt gcgcgaggtg ctggggctga tggcggccct
     3421 gttcggcatc agttgcagca t
//
LOCUS       DASUYC010000406         2260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_232661,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000406 DASUYC010000000
VERSION     DASUYC010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232661"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="VF276_06700"
     CDS             complement(<1..811)
                     /locus_tag="VF276_06700"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6606589.1"
                     /translation="MIVDAKTKNPQRTAPARVISPMQEAGEQPAAVPRLVGRLGPALA
                     SLGAGLLLWQLLAMVAPNPPTLLPGPGVVAARLWDLAVRGILWHHVSVTLTEAGLGFL
                     LAAVLGLALGYPVAKSRLLEALLAPYVAGTQAVPIVAIAPLMVLWFGLDLLPKVLTCA
                     VIVFFPILVNTIIGLRAVDRALIEAAHSMGAGPWQTLRYVEVPLALHSILGGLKLGIT
                     LAMTGAVVGEFIAANAGLGYLLTYGRSIYDTPLVFAALVSLAAIASLGYGLI"
     regulatory      complement(1004..1137)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1299..1880)
                     /locus_tag="VF276_06705"
     CDS             complement(1299..1880)
                     /locus_tag="VF276_06705"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX6606590.1"
                     /translation="MQVAPVTLQGREIRLEPLTLDHAPALWRAADPAIFTYIPHPVDD
                     TLPAFKTYLTGLLRAPDYQPFAMVRQETGQPVGVTCYMDIRPAHRGLEIGSTWIARPY
                     QGTRVNPESKYLLLRHAFEELGAVRVQLKTDGRNRHSQAAIAKLGAQREGVLRKHLIL
                     QDGYIRDTVMFSILAEEWPAVKAGLEARLGYAP"
     gene            2063..>2260
                     /locus_tag="VF276_06710"
     CDS             2063..>2260
                     /locus_tag="VF276_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="produces methionine from
                     2-keto-4-methylthiobutyrate and glutamine in vitro;
                     mutations do not affect methionine salvage in vivo
                     however; Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HEX6606591.1"
                     /translation="MKLAARISNLPPYVFASMGKRIAERRARGDQVINFGMGDPDVPM
                     PENLVDAVCDAAHDVRTHRYPN"
BASE COUNT          377 a          753 c          774 g          356 t
ORIGIN      
        1 tgatcaggcc gtagcccagg ctggcgatgg cggccaggct caccagcgcg gcaaagacca
       61 gcggcgtgtc gtagatcgag cggccatagg tcagcaggta gccgagcccg gcgttggccg
      121 cgatgaactc gcccacgacc gccccggtca tggcgagcgt gatgcccagt ttgaggccgc
      181 cgaggatgct gtgcagggcc agcggcacct ccacatagcg cagcgtctgc cacggcccag
      241 cacccatgct gtgcgcggcc tcgatcagcg cacggtccac ggcgcgcagg ccgatgatcg
      301 tgttgaccag gatggggaag aagacgatca ccgcgcaggt gagcactttg ggcagcagat
      361 cgaggccgaa ccagagcacc atcagcggcg cgatcgccac gatgggtacg gcctgggtgc
      421 cggcgacata gggcgccagc aacgcttcca gcaggcgcga cttggcgacg gggtagccga
      481 gggccagccc cagcaccgcc gccagcagga aaccaagccc cgcttcggtc agtgtgacgc
      541 tgacgtggtg ccagaggatg ccgcggacgg cgagatccca gagccgcgcg gccaccacgc
      601 ccggtcccgg cagcagggtc ggcgggttgg gcgccaccat cgccaggagt tgccagagca
      661 gcaagcccgc ccccaacgag gccagcgccg ggccgagccg ccccaccagg cgcggcaccg
      721 cggccggttg ttcgccggct tcctgcatcg ggctgatcac gcgggccggg gcggtccgct
      781 gggggttctt ggtcttcgca tccacgatca tcgcgttctc cgaatccgat ctggggcata
      841 aaaacaccct ggccggcgac agggaccagg gtactgttgc ggatggattc ggattcgcca
      901 gacggatgcg tggccctgcg gccctcctgg gctgcccggc ggaagcgcat agcgacgcag
      961 ccaaccggcg cggccacccg tgggggacgc agcacgaacc cggcgcgatc ttcttccatc
     1021 cggactatac cgtcggcgac ggctttgcac cgtctcctac cgccgaagcg gctcgcgggc
     1081 tggccccttg cgaggcttac cgccgatcgg gaattgcacc ctgccctgaa gatctgcttg
     1141 cctgttacgg ctcatactca gtatacgcag ccttgccggg cgtgtcaaga cggcatcggg
     1201 ccataatctc accgcgaagg cgcgaaggac gcgaaggttc ttgatattag tcgcgttctt
     1261 cgcgttcttc gcgccttcgc ggtgcattta tggtccggct agggcgcgta gccgaggcgg
     1321 gcttcgaggc cggctttcac cgcgggccac tcctcggcga ggatgctgaa catgaccgtg
     1381 tcgcggatgt agccgtcctg caagatcaag tgcttgcgca gcacgccctc gcgctgcgcg
     1441 ccgagcttgg cgatggccgc ctggctgtgc cggttgcgcc cgtcggtttt gagttgcacg
     1501 cgcacggcgc ccaactcctc gaaggcatgg cgcagcagca ggtacttgct ctcggggttg
     1561 acgcgggtgc cctgatacgg gcgggcgatc caggtcgagc cgatttccag gccgcgatgc
     1621 gccgggcgga tatccatata gcaggtgacg ccaacaggct gcccggtctc ctggcgcacc
     1681 atcgcgaacg gctggtagtc gggtgctcgc aagaggcccg tgagataggt cttgaacgcc
     1741 ggcaacgtgt cgtccacggg gtgggggata taggtgaaga tagccggatc ggcggcccgc
     1801 cagagggcgg gcgcgtggtc cagcgtgagc ggctccaggc ggatctcgcg cccctggagc
     1861 gtgacgggtg ctacctgcat atgcttgaaa cctccctggc ccctattata cgcgggcgct
     1921 gtaagatccg caggccaccg ggcggctcaa gccaccgggc cggggccgcc tgtggtatac
     1981 tgggggatac agcgcagtgt tgccctgccg tgccactccc tgggcgcggc agcatgaacc
     2041 cgttcaaagg agaacgtgcc ccgtgaaact tgccgcccgc atcagcaatc tcccgcccta
     2101 cgtattcgcc tctatgggca agcgcatcgc cgagcgccgc gcccgcggcg accaggttat
     2161 caacttcggc atgggcgacc ccgatgtgcc gatgccggag aacctggtgg acgccgtctg
     2221 cgatgcggcc cacgacgtgc ggacgcatcg ctaccccaat
//
LOCUS       DASUYC010000407         2555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1408290, whole genome shotgun sequence.
ACCESSION   DASUYC010000407 DASUYC010000000
VERSION     DASUYC010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408290"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            509..1864
                     /locus_tag="VF276_06715"
     CDS             509..1864
                     /locus_tag="VF276_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX6606592.1"
                     /translation="MAVQVAAKPVPTFPEPPLLGSMRAFQKDRLTLLLRGMQACGPVA
                     RIHFGPFPMLVLAAPDLVQAALVDHAADFDKGVVLHRAFTPVVGQGLINNEGESWRQQ
                     RKLMAPAFTHRHIAGYADTMVAYTERAQQAWATGEVLRIDQEMMRLTMSIVGKTLFDA
                     DVFDESDELGVAVTDALAFLQYTISHLFPVPLSWPLPRNARTRRALAVIDRRLQAMID
                     ERRASGVDRGDFLSLLLAARDEAGQGMSDRQIRDEAITLFLAGHETTANTLAWAWYLL
                     GRNPEVYAKLQQEADTVLGGRPATVADVPHLPYALQVFKEALRLHPPADSMSRTALRD
                     VDLAGYPVRKNGIIVIPIYAMHRNPAYFPDPDRFDPERFTPENEKRLPRHVYMPFGAG
                     PRICIGNHFALMEGQLILATLAQRVRFAVPASEHPVPEPLFAVRMKGGCRVVVHRRAA
                     G"
     gene            complement(2004..>2555)
                     /locus_tag="VF276_06720"
     CDS             complement(2004..>2555)
                     /locus_tag="VF276_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606593.1"
                     /translation="AQRWGQDTTCSKWGFVQGVHERGEGKNWKAYNCCNSWPWALEDY
                     ARNSLGLNGAASCHAPHQRQIATVSWGEKEVQVLCSDTIPGDKFGEREVKSKFCTGKI
                     DKEVIELSPKAMQDLSDQTLNALGVRVCYSGTQQDLCLHNGPGPAARTGRDAFPECSD
                     CLTAGCPVPEGTPKLKDTGWPRA"
BASE COUNT          385 a          883 c          842 g          445 t
ORIGIN      
        1 gccgggctgt gtgcccgccc ccgcgccgct gccggccccc cgttgtcatg ctgcacgcag
       61 tgaagcatcg cgtctccgtg gcgccgcgac tcttcgctgt gctcagtaat ctgtcacatg
      121 gtttttgtca ctctgagcgg agcgaagagt ctcgtcttgg acccgaacga gacgagattc
      181 ttcgctccgc tcagaatgac aatttgagtg tgacgctcta ctcagagtga caagcccccc
      241 gcgccgcgcc aagactcttc gccgcgctca gagtgacaag cccagaccgt cgcccgtctc
      301 ctgacggctg acggctgacg gctaacggct gaccactgac cactgaccac tgaccactcg
      361 tctccgcact cgcgctggga caggcgccgc gccggggcta catctttcga ccgattgacg
      421 cggcgcgcaa ccggcttata cttagcttat aagaattgtg cgaaatttca caattcagcc
      481 ggttaggctg aagaacggag tgtgtgccat ggctgtccag gttgccgcga agccggtccc
      541 gacgtttccc gagccgccgc tgctcggcag tatgcgcgct ttccagaagg atcggctgac
      601 cctgttgctg cgggggatgc aggcgtgcgg cccggtggcc cgcatccatt tcggcccgtt
      661 tcccatgctg gtgctggccg cgcccgacct ggtccaggcc gcgctggtgg accacgcggc
      721 cgatttcgac aagggcgtgg tcctgcaccg cgccttcacc cccgtggtgg ggcaggggct
      781 gatcaacaac gagggcgaaa gctggcggca gcagcgcaag ctgatggccc ccgcctttac
      841 ccaccgccac atcgcgggct acgcggacac catggtggcc tataccgagc gcgcccagca
      901 ggcctgggcc acaggcgagg tgctgcgcat cgaccaggag atgatgcggc tgaccatgag
      961 catcgtcggc aagacgctgt tcgacgccga cgtgttcgac gagagcgacg aactgggcgt
     1021 ggcggtgacg gatgccctgg ccttcctgca atacaccatc agccacctgt tcccggtgcc
     1081 gctgagctgg ccgctgccgc gcaatgcccg cacccgccgc gccctggccg tgatcgaccg
     1141 gcggctgcag gccatgatcg acgagcgccg ggcgagcggc gtggaccggg gcgacttcct
     1201 gtcgctgctg ctggccgccc gcgacgaggc cggccagggc atgagcgacc ggcagatccg
     1261 cgacgaggcc atcaccctgt tcctggccgg gcacgaaacc acggccaaca cgctggcctg
     1321 ggcctggtac ctgctgggcc gcaacccgga ggtctacgcg aagctccagc aggaggccga
     1381 tacggtgctg ggcgggcgcc cggccacggt ggccgatgtg ccccacctgc cctacgcgct
     1441 gcaggtgttc aaggaggcgc tgcggctgca cccgcccgcc gactcgatga gccgcacggc
     1501 cctgcgcgac gtggacctgg cgggctatcc ggtgcgcaag aacggcatca tcgtcatccc
     1561 gatctatgcc atgcaccgca acccggccta cttcccggac cccgaccgct tcgacccgga
     1621 gcggttcacg cccgagaacg agaagcggct gccgcgtcac gtttacatgc ccttcggcgc
     1681 cgggccgcgc atctgcatcg gcaaccactt cgcgctgatg gagggccagt tgatcctggc
     1741 gacgctggcc cagcgcgtgc gcttcgcggt gcccgcgagc gagcacccgg tgcccgagcc
     1801 gctgttcgcc gtgcgcatga aaggcggctg ccgcgtcgtc gtccatcggc gggcggccgg
     1861 ctgatccgcg cttcctggcg gccttatcac tctgagcgca aggcttgtca ctctgagcgc
     1921 agcgaagagt ctcgtctcgt gcgggtacaa gacgagatgc ttcactgcgt tcagcatgac
     1981 aacgccggct ggaccgaggg cgatcaggcg cgcggccagc cggtgtcctt gagcttcggc
     2041 gtgccctcgg gcaccgggca gcccgcggtg aggcagtcgc tgcattcggg gaaggcgtcg
     2101 cggccggtgc gtgccgccgg gccggggccg ttgtgcaggc acaggtcctg ttgggtgccc
     2161 gaatagcaga cgcgcacccc gagcgcgttc agcgtctggt cggagaggtc ctgcatcgcc
     2221 ttggggctta attcgatcac ttccttgtcg atcttgcccg tgcagaactt ggacttgacc
     2281 tcgcgctccc cgaacttgtc gccggggatg gtgtccgagc agagcacttg cacttccttt
     2341 tcgccccagc tcaccgtggc gatctggcgc tggtgtggcg cgtggcagga ggccgcgccg
     2401 ttgaggccca ggctgttgcg cgcgtagtct tccagcgccc agggccagct attgcagcag
     2461 ttgtacgctt tccagttctt gccctcgccc cgttcgtgga cgccctgcac gaagccccac
     2521 ttcgagcagg tggtgtcctg gccccagcgc tgggc
//
LOCUS       DASUYC010000408         3229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_699942,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000408 DASUYC010000000
VERSION     DASUYC010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699942"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..1326
                     /locus_tag="VF276_06725"
     CDS             112..1326
                     /locus_tag="VF276_06725"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6606594.1"
                     /translation="MRKLWVIAGQEFAAHVRRRTFLLTTLGLPLLLILVFALIIGLTI
                     NSQNVKAIGYVDHAGLLSDLPVQVLVPGDSDSGFGAGPVPAIRYPDEAAARQALDGKQ
                     IDVYFVVPADYRATGNVTGYAATAIPQVGQDRFTRLLRQSLLAAIPGDDPRLASPIAD
                     TVNLRADGRPNNPAAAVLVPFLFAFLFYLASYIAGSYLMQSVAEEKESRVMEILATSV
                     RPGDLMGGKILGLGLVGLVQMAVWVGVGLLTLWVGRAYVPFLADVSLPWDTVLLIVAL
                     FVPSYLLFAASLAAVGAMVTARQEGQQLSGIFSLLGAVPIWFFISIITDPTGPLALGL
                     SFFPYTAPMTLLLRQAAMTVPVEQIVLAWAITAGAALGMIWVAGRVLRFGMLRYGKRL
                     GLRELGQALRGA"
     gene            1331..2575
                     /locus_tag="VF276_06730"
     CDS             1331..2575
                     /locus_tag="VF276_06730"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6606595.1"
                     /translation="MNKTLTVAAREFLTHLQKPSFYLATIGIPLFIALIMFLSNQGRT
                     NDALSQQGLGGIGQLLTAQGAQPFGYTDASGTITTIPAVLPPGFVKAYPDEAAGRAAL
                     TAGTISAFYVIPADYRVSGRIVHYTRGVEPGNGVTSQQVLRLVLLTNLLPDQDPRTAL
                     RLLEPMRLQAVAVAGAGGGAGDLGGQNLLLVTGFALLLTYAIFMSSGILLQALLEEKE
                     NRIMEVLLTSGSARQLLTGKVLGLGALGLIQLLIWLGLAYVLTGSGASFVAALGTVNV
                     PLAVWGLAVVYFLLGYLFFAALMAGIGAISPSRRESSQYAGVVSMLAIVPLFLLAPLA
                     ADPNGPLAVFLSIFPPTAPAIMLARLAVTTVPPWQVAVSLAVLALTVVGGIAAAARLF
                     RAGTLLQGTRLSWREVRRALAG"
     gene            complement(2620..>3229)
                     /locus_tag="VF276_06735"
     CDS             complement(2620..>3229)
                     /locus_tag="VF276_06735"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX6606596.1"
                     /translation="SQLNCVLLALRPQGPPGDPFAWQPGLLLPILLTGAVGTLAGASL
                     LVALDPSLALTCLGGLVVLFVLTDRVRRDWHPNPRHERPLALAVGLVTGVLSGLAGVS
                     GPTLVPYLHSRKLDKHQFVTYLNMLFLCFGAYQWIVFFFVGLYTWARVVEAASFIPAA
                     LLGIGLGARVRNRVSAAFFNRLVLLILFVTGLDLIRRGLHLF"
BASE COUNT          466 a         1047 c         1172 g          544 t
ORIGIN      
        1 aactggcgct gcaacccgcc gtcacggtcg atcgcttcga ggtggcgacg ccctcactcg
       61 acgaggtgtt cgtgcgcgtc gtgcagggcg aggcgcccag ggaggccggc catgcgtaaa
      121 ctctgggtca tcgccgggca agaattcgcg gcccatgtcc ggcggcggac gtttttgttg
      181 accacgctgg gcctgccgct gctgctcata ctcgtgtttg cgctgatcat cggcttgacg
      241 atcaattcgc agaacgtcaa ggcgatcggc tacgtggacc acgcaggcct cttgagtgat
      301 ctgccggtgc aggtgctggt gcccggcgat tcggacagtg gcttcggcgc cggccccgtg
      361 cccgcgatcc gctacccgga tgaagcggcg gcgcgccagg cgctcgacgg caagcagatc
      421 gatgtctatt ttgtcgtgcc cgccgactac cgggcgacag gaaatgttac cggctacgcg
      481 gcgaccgcga ttccgcaggt gggccaggat cgcttcaccc gcctgctgcg ccagagcctg
      541 ctcgccgcca ttcccggcga cgacccccgc ctggcctcgc ccatcgccga caccgtgaat
      601 ctgcgggccg acgggcggcc caacaacccc gccgctgccg tgctggtgcc gttcctgttc
      661 gccttcctgt tctacctggc gagctatatc gccgggtcgt acctgatgca gagcgtggcc
      721 gaggagaagg agagccgggt gatggagatc ctggcgacct cggtgcgccc cggcgatctc
      781 atgggcggca agatcctggg cctgggtctg gtcgggctgg tgcagatggc tgtctgggtg
      841 ggcgtggggc tgctcaccct gtgggtgggg cgggcctatg tgcccttcct ggccgatgtg
      901 tcgctgccgt gggacacggt gctgctgatc gtcgccttgt ttgtgcccag ctacctgctg
      961 ttcgcggcgt cgctggcggc ggtaggcgcg atggtcacgg cgcggcagga aggccagcag
     1021 ctttcgggca tcttcagcct gctgggcgct gtgccgatct ggttcttcat cagcatcatc
     1081 accgacccga ccgggccgct ggcgctgggc ctgagcttct ttccctatac cgcgccgatg
     1141 acgctgctgt tgcggcaagc cgccatgacc gtcccggtcg agcagatcgt gctggcgtgg
     1201 gcgatcacgg cgggcgccgc gctgggcatg atctgggtcg ccgggcgggt gctgcggttc
     1261 ggcatgctgc gttacggcaa gcgcctgggt ctgcgtgagc tgggccaggc attgcggggg
     1321 gcatgagacg atgaacaaga cattgacggt ggcggcgcgc gagttcctga cccacctgca
     1381 aaaaccgagc ttctacctgg cgacgatcgg catcccgctc ttcatcgcgc tgatcatgtt
     1441 cctgtccaac caggggcgca cgaacgacgc gctgagccag caaggcctgg gcgggatcgg
     1501 ccaactgctg accgcgcagg gcgcgcagcc cttcggctac accgacgcca gcggcacgat
     1561 cacgacgatc ccggcggtgc tgccgcccgg cttcgtcaag gcctatcccg acgaggccgc
     1621 cgggcgcgcg gccctcacgg cgggcaccat cagcgcgttc tacgtgatcc cggcggacta
     1681 ccgggtgagc gggcgcatcg tccactatac ccgcggtgtc gagccgggca acggagtgac
     1741 gagccagcag gtgctgcgcc tggtgctgct gacgaacctg ctgcccgatc aggacccacg
     1801 cacggccctg cgcctcctgg agccgatgcg gctccaggcg gtggccgtgg ccggtgccgg
     1861 cggcggcgcg ggcgacctgg gcgggcaaaa cctgttgctg gtgaccggct tcgcgttgct
     1921 gctgacctat gccatcttca tgtcgtcggg gattctgttg caggcgctgc tggaagagaa
     1981 ggaaaaccgc atcatggagg tgctgctgac ctccggctcg gcccgacaac tgctgaccgg
     2041 caaggtattg ggcctgggcg cgctgggcct gatccagttg ctgatctggt tggggctggc
     2101 ctacgtgctg accgggtcgg gcgcctcgtt cgtggcggcg ctgggcacgg tgaacgtgcc
     2161 gctcgcggtg tgggggctgg cggtggtcta cttcctcctg ggctacctgt tcttcgcggc
     2221 gctgatggcc ggcatcgggg caatcagccc gtcgcggcgc gaaagctcgc aatacgcggg
     2281 cgttgtctca atgctggcga tcgtgccgct gttcctgctg gcgccgctgg cggcggaccc
     2341 gaacggcccg ctggccgtct tcctgagcat cttcccgcct acggcgccgg cgattatgct
     2401 ggcgcgcctg gcggtgacca cggtgccgcc gtggcaggtg gcggtgagcc tggcggtgct
     2461 ggcgctcact gtggtgggcg gcatcgcggc ggcagcgcgg ctgttccgtg cgggcacgct
     2521 gttgcagggc acacgcctct cctggcgcga agtccgccgg gcgctggccg ggtaggcgcc
     2581 tggtgtggac cgcaaaggct gtaccggggc aaatggacct cagaacagat gcaggccccg
     2641 ccggatcagg tccagcccgg tcacgaagag gatcagcagc accagccggt tgaagaaggc
     2701 ggcgctgacg cggttgcgga cgcgggcgcc cagcccgatg ccgagtaggg ccgccgggat
     2761 gaagctggcc gcttcgacca cgcgcgccca ggtgtagagg ccgacgaaga agaagacgat
     2821 ccactggtag gcgccgaagc agaggaacag catattcagg taggtgacga attggtgctt
     2881 gtcgagcttg cggctgtgca ggtagggcac cagggtgggg ccgctgactc cggccaggcc
     2941 gctgagcacg ccggtgacca ggccgacggc cagggccagg ggccgctcgt ggcgcgggtt
     3001 gggatgccag tcacggcgga cccggtcggt gagcacaaag agcaccacca gcccgcccag
     3061 gcaggtgagc gccagcgacg gatcgagcgc caccagtagg ctcgccccag ccagcgtgcc
     3121 caccgcgccg gtgagtaaga tcggcagcag caggccgggc tgccacgcga acggatcgcc
     3181 gggcggccct tgcgggcgca gcgccagcaa cacgcagttc aactggctg
//
LOCUS       DASUYC010000409         4373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1261345, whole genome shotgun sequence.
ACCESSION   DASUYC010000409 DASUYC010000000
VERSION     DASUYC010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261345"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(287..628)
                     /locus_tag="VF276_06740"
     CDS             complement(287..628)
                     /locus_tag="VF276_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012406963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606597.1"
                     /translation="MAARELHLELLLSKTVRGITGEPVGRIEEVRARQEGDEWVVHEF
                     LIGAGAVVSRLSAGRLGHVVAPLFGHRDAPHGYRVRWDQIDLSDSDHPRLRCRRDQLR
                     VFVPEVTSPHK"
     gene            complement(628..987)
                     /locus_tag="VF276_06745"
     CDS             complement(628..987)
                     /locus_tag="VF276_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015163241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606598.1"
                     /translation="MDLIKDVLDNQLVDRKDRKLGKVDGIVVELRDGLPPRLLRIEVG
                     PSTVARRIHPRFGAWVAARLRRWNTIRSEPSNIPWGAIQEVGINVKLDRDGAMTTTLA
                     WEKWLSDHIIRRIPGGA"
     gene            complement(988..2205)
                     /locus_tag="VF276_06750"
     CDS             complement(988..2205)
                     /locus_tag="VF276_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEX6606599.1"
                     /translation="MKKFLNVALGIVTSIGGFLDVGSIATSAQAGAAFGFSLIWAIVL
                     GTICVSFLVEMTGRLGAISKHTLGDALRERFGAKFFMLPYLVEYVVDFLLLASEIGGV
                     CIALQLATGIGFRWWALPVALAVWLLLWRGTFGLIENGVASLGLVTLVFIAGSFALHP
                     SLAAVGAGLLPSLPRHDTAHYLFIAVSIIGAVITPFLLYFYSSGAIEDKWSEDDLGTN
                     RMVAGLGMSFGGMVSLAVLVVSGAVLFPRGIQMDSYEQAVLGLVVPFGGWGFALFIAA
                     LAIACFGACLEVSLSTAYLTAQGFGWNWGESQKPNQDARFSLVYTCNIFLASLLMLAG
                     INPLQLTLFTMALIATMLPVVVIPLLILMNDKRYLREYRNGWISNGVVVFVIVLTFVL
                     ALISIPLEILGGS"
     gene            complement(2205..2624)
                     /locus_tag="VF276_06755"
     CDS             complement(2205..2624)
                     /locus_tag="VF276_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606600.1"
                     /translation="MAWRIIVLVALLGALTACGNQSPAGDQAREIETLLSWTATGQLA
                     ADSWRQGKVPQIYTTRTLQYAAEQLDSEQPAVAKLPPIAGNTPLTERLQRVRTAIAQM
                     RTAVEQGDRAALAAPLQQITTEETAFHQLLSNLEGEQ"
     gene            complement(2779..3822)
                     /locus_tag="VF276_06760"
     CDS             complement(2779..3822)
                     /locus_tag="VF276_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606601.1"
                     /translation="MSLTEDLVRKISQTEHQYRRRVNLMAGIYVLLNITAIISIVIII
                     WYYKLFITVAQRSNMETLTLAIIIVLFSYLVVTTFAGLIGACKMVFYNLPLGGGAETA
                     KQDGAGKRKQDPGKEEKAGLPPGQRKIEERKQAALKPAKDEVSKAYFNIALEAEDKPQ
                     EPIRIPVEDEAGKLGEMVIDGAEARFEAEKKGISNTIFEYLLNQIQDCLRERDPELDM
                     QIVAWSTIDDEKAAQYRSQVQAFRTLAAQLEKGPIWPTCNLTRGNVAYIRTKLREIVP
                     YLRNEAFLPDVEYRAEYNLPIIPEPLGFVSLRRTEQRADPVATMGCASLVTLGVMALL
                     ILFLFFPPWLPSK"
     gene            3985..>4373
                     /locus_tag="VF276_06765"
     CDS             3985..>4373
                     /locus_tag="VF276_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606602.1"
                     /translation="MRWFSRLSAATRAAGGPQWRARAALALLVPVMALLLTGCLGPKP
                     IIAGVTKQPPAAPDQPYRLQVLVRNDGPGSGQVQVSVRFVDKQTQRVILANDQEVQLE
                     HEEQSVNFELNLPPTAPPLDQLDVQVDT"
BASE COUNT          763 a         1435 c         1410 g          765 t
ORIGIN      
        1 tgctacctgg cggcaacacc gccctgatct acgccgccgg cagcgcggca acctctcccc
       61 gcgccgcttt ctacaccgtc ccgctggacg gcagcgcgcg cacgcaggcc tttgccctgc
      121 ccgacggcgc tttctacgcc cccgtcacgg tgccctgagc cgtttttcca gtcgtcattg
      181 tcactctgag cgaagcgaag agtctcgtcg ccagggagac gagatgcttc actgcgttca
      241 gcatgacaag ttgggctgga agcagtactg aggccgcggc gcccggctac ttgtgcggcg
      301 acgtcacctc ggggacaaag acgcgcagtt gatcgcggcg gcagcgcagg cgcggatggt
      361 cggagtcgct gaggtcgatc tggtcccagc gcacgcggta gccgtgcggc gcgtcgcggt
      421 ggccgaacag cggggcgacg acgtggccca gccgcccggc agatagccgg ctcaccactg
      481 cgcccgcccc gatcaggaac tcgtggacca cccactcgtc gccctcctgg cgggcgcgca
      541 cttcctcgat ccgccccacc ggctcgccgg tgatgccgcg caccgtcttg ctcagcaaca
      601 actccaggtg cagttcgcga gccgccatca cgcgcctccg gggatgcgcc ggatgatgtg
      661 gtcgctgagc cacttctccc aggccagcgt cgtcgtcatg gcgccgtcgc ggtccagctt
      721 gacgttgatg ccgacctcct ggatcgcccc ccaggggatg ttgctcggct cggaccggat
      781 cgtgttccag cgccgcaacc gggccgctac ccaggcgccg aaccgcggat ggatccgccg
      841 ggccacggtg cttggcccga cctcgatgcg cagcagccgc ggcggtaacc cgtcgcgcag
      901 ctccacgacg atgccgtcca ctttgccgag cttgcggtcc ttgcggtcca ccagttggtt
      961 gtcgagcacg tccttgatca ggtccatcta gctgcctccc agaatctcca gtggaatgga
     1021 aatcagcgcc agcacaaacg tcagcacgat gacgaagacg acgacgccgt tggaaatcca
     1081 cccgttgcga tactcgcgca gatagcgttt atcgttcatc aaaatgagca acgggatcac
     1141 cacgacgggc aacatggtgg cgatcagcgc catggtgaac agcgtcagtt gcagggggtt
     1201 gatcccggcc agcatcagca gcgaggccag gaaaatgttg caggtgtaga ccaggctgaa
     1261 ccgggcgtcc tggttgggtt tctggctctc gccccagttc cagccgaacc cctgcgccgt
     1321 taggtaagcg gtgctcagcg acacctccag gcacgcgccg aagcaggcga tggcgagcgc
     1381 ggcgatgaag agggcgaagc cccacccgcc gaagggcacg accagcccca gcacggcctg
     1441 ttcatagctg tccatctgga tgccgcgcgg aaagagcacc gcgcccgata cgaccagcac
     1501 ggcgagggac accatgccgc cgaagctcat gcccagcccc gccaccatgc ggttcgttcc
     1561 gagatcgtcc tcgctccatt tatcttcgat ggcgccggag gaatagaagt agaggaggaa
     1621 cggcgtgatc accgcgccga tgatgctgac ggcgatgaac aggtagtgcg ccgtgtcgtg
     1681 gcggggcagg ctgggcagca ggcccgcgcc gaccgcggcc agggacggat gcagggcgaa
     1741 cgacccggcg atgaacacca gcgtgaccag ccccaggctc gccaccccgt tttcgatcag
     1801 gccgaaggtg ccgcgccaga gcaacagcca gacggccagc gccaccggca acgcccacca
     1861 gcgaaacccg atgcctgtcg ccagttgcag cgcgatgcac acgccgccga tctccgaggc
     1921 cagcaacaga aaatccacca catactcgac caggtagggc aacatgaaaa acttggcccc
     1981 gaatcgttcg cgcagcgcgt cgcccagtgt gtgcttgctg atggcgccca gccgcccggt
     2041 catctccacc aggaagctga cgcagatcgt gcccagcaca atggcccaga tcagcgagaa
     2101 gccgaacgcg gccccggcct gtgccgaggt agcgatggac cccacgtcga ggaacccgcc
     2161 gatgctggtc acgatgccca gcgcgacatt gaggaacttc ttcattattg ctcgccctcc
     2221 aggttactca gcaactggtg gaaggcggtt tcttccgtgg tgatctgctg caacggcgcg
     2281 gccagcgcgg cgcggtcgcc ctgctcgact gcggttcgca tctgggcgat ggcagttcgg
     2341 actcgctgaa gccgttcggt gagcggggtg ttcccggcga tgggcggtag ctttgccacc
     2401 gccggctgct ccgagtccaa ttgctccgcc gcgtattgca gggtgcgggt ggtgtagatc
     2461 tgaggcactt tcccctgccg ccacgagtcg gcggccagtt gcccggtggc cgtccaggac
     2521 agcagagtct cgatttccct agcttgatcg cctgcgggcg actggttccc gcacgcggtg
     2581 agggcgccca gcagtgcgac gagtacgatg atgcgccagg ccatgctcgg tgggtctcct
     2641 ttgggccgcg gttccataag ggtagaacag acccggcaac ccaaatgtga caagggggat
     2701 cgtggtggta tggggagagg caccggcgga cgcctgccac gcgccggggt cggcgggctg
     2761 aggccgagcg cgccgggtct atttgctggg cagccagggc gggaagaaga gaaacaggat
     2821 cagcagggcc atgacgccga gcgtgaccag cgaggcgcag cccatggtcg ccacggggtc
     2881 ggcccgttgc tcggtgcgcc gcagcgagac gaaccccagc ggctccggga tgatcggcag
     2941 gttgtactcg gcccggtatt cgacatcggg caagaaggct tcgttgcgca ggtaaggcac
     3001 gatctcgcgt agcttcgtgc ggatataggc gacgttgccg cgcgtcaggt tgcaggtcgg
     3061 ccagatcggg cctttctcca actgagcggc cagggtgcga aatgcctgca cctggctgcg
     3121 atattgcgcc gccttttcat cgtcgatggt ggaccaggcc acgatctgca tatccagctc
     3181 cgggtcgcgt tcgcgcaggc agtcctggat ctggttcagc aaatactcga agatcgtgtt
     3241 gctgatgccc ttcttctcgg cctcgaagcg ggcttccgcc ccgtcgatca ccatctcccc
     3301 cagcttcccg gcttcgtcct ccaccgggat gcggatcggc tcttgaggct tatcttcggc
     3361 ttccagagcg atgttgaagt aggctttgct gacctcgtcc ttggccggtt tcagcgccgc
     3421 ctgcttgcgc tcctcgatct tgcgctgccc cggcggcagg cccgccttct cttccttgcc
     3481 ggggtcctgc ttgcgcttgc cggccccgtc ctgcttggcc gtctccgcgc cgccgcccag
     3541 cggcaggttg taaaacacca tcttgcaggc cccaatcaac ccggcgaagg tcgtcaccac
     3601 caggtagctg aacaggacga tgatgatcgc cagggtcagc gtttccatgt tgctgcgctg
     3661 cgccacggtg ataaagagct tgtagtacca gatgataatc acaatgctga tgatggccgt
     3721 gatgttcagc agcacataga tcccggccat cagattgacc cggcggcgat actggtgctc
     3781 ggtttgcgaa atcttgcgga ccaggtcttc ggtgaggctc acaggcggct tgctccacta
     3841 tgcataggga tagatcgaat cgtgtgtgcg gcttcttgcg ttgtaacgat atgatcgcat
     3901 atgttgtgac tatcagacag ggtatatccg cttccggccc gccgcgccgg gggatagttt
     3961 gagaaaaagg agcatacatc cctgatgagg tggttctcgc gattatccgc agcgacccgc
     4021 gcggcgggcg gccctcagtg gcgcgcgcgg gcggcgctgg cgctgctcgt gcccgtcatg
     4081 gcgctgctgc tgaccgggtg cctgggtccc aagcctatca ttgccggcgt gaccaagcag
     4141 ccgcccgccg cgcccgacca gccctaccgc ctccaggtgc tggtgcgcaa cgacgggccg
     4201 gggtcggggc aggtccaggt ctcggtgcgc ttcgtggaca agcagacgca gcgcgtgatc
     4261 ctggccaacg accaggaggt gcagttggag cacgaggaac agagcgtgaa tttcgagcta
     4321 aacctgccgc ccaccgcgcc cccgctcgat caactcgacg tgcaggtgga tac
//
LOCUS       DASUYC010000410         2333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_940961,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000410 DASUYC010000000
VERSION     DASUYC010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940961"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VF276_06770"
     CDS             <1..485
                     /locus_tag="VF276_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606603.1"
                     /translation="LSPAAQAACIAQVAARAANLSTREIEKATEPVYRYQRYTRPETY
                     RILDQLDNVSTPRAMLRLMRFLRAGGGLPPALAAEMHSILARGDGGRDAGCFHGKVRA
                     WGGKGGSDLSQASLNGYAEAPDGCILVYSLLSSHLHDDYRGAERLCQALDALYQALTA
                     "
     gene            544..1353
                     /locus_tag="VF276_06775"
     CDS             544..1353
                     /locus_tag="VF276_06775"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX6606604.1"
                     /translation="MLSGKIRLFLGGLGLLALVAGGAVTAGDLARPAAQQAGTTYIVQ
                     PGDTLAGSAYRAYGDAAAWTCIQAANPAIRTAADLQLGMSITLPSKAACAAGAPQPPT
                     PAAAQSTPTARPTAAPARPTAPPTTAPATGTPPPATPTSPPTAGASVYVIRSGDTLSS
                     IAWRVYGNPGAWPCIWAANQWLTNPRFLLRGWQITLPPAATCDPPAPTPAAKATAAPA
                     ARYHVVVSMETLSGIACRYYTDCNYWRIYNANRDKIWNVNFILPGTVLRIP"
     gene            1546..>2333
                     /gene="mltG"
                     /locus_tag="VF276_06780"
     CDS             1546..>2333
                     /gene="mltG"
                     /locus_tag="VF276_06780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX6606605.1"
                     /translation="MSTVLRLLRYLLGIVVLVCVIAAGITIYAFTDLNRAAGPAAAPQ
                     QLFTIDQGESVASIAARLEQHGLINSATLFRIDLKLRGAEERIKAGDFQLSAGMTPQQ
                     IIEAITTAPSATMVQVLIPEGWRIGQIADKLAELDLVDRDRFISLTMTGTFQYDFLPD
                     RPAGGEIEGYLFPDTYGFPRHGTDREQRVLDIMLTNFGKRVTPEMRKDATATGYTLHQ
                     IVTLASIVEREAQLDSERARIAGVFYNRLKAGMKLDADPTTQYAL"
BASE COUNT          382 a          870 c          715 g          366 t
ORIGIN      
        1 ccttatcgcc tgcggcccag gcagcctgca tcgcgcaggt tgcggcgcgc gccgcgaacc
       61 tgtctactcg cgagatcgag aaggccaccg agccggtcta tcggtatcag cggtacaccc
      121 gccccgaaac atatcgtatc ctcgatcagt tagacaatgt gagtacgccg cgcgccatgc
      181 tgcgcctgat gcgctttctg cgcgccgggg gcggcttgcc gcccgcgctg gccgccgaga
      241 tgcacagcat cctggcccgc ggcgacggcg ggcgcgacgc cgggtgcttt cacggcaagg
      301 tccgcgcgtg gggcggcaag ggtggcagcg acctgagcca ggcgtccctg aacggctatg
      361 ccgaagcgcc cgacgggtgc atcctggtgt acagtctgtt gagcagccat ctgcacgacg
      421 attatcgcgg ggccgagcgc ctatgccagg ccctggacgc gctctatcag gcgttgaccg
      481 cctagttggg ggcagccgcg ccggacgact ctacgcgcag cgttcgcgta aggaggccgg
      541 aagatgctca gcggcaagat caggctcttc ctcggcgggc tgggactcct ggccctggtg
      601 gccggcggcg cggttacggc gggcgacctc gcgcgcccgg cggcccagca ggcgggcacg
      661 acgtacatcg tgcagcccgg agataccctg gccggtagcg cctaccgcgc ctacggggac
      721 gcggcagcct ggacgtgcat ccaggcggcg aaccccgcca tccgcacagc cgccgacctg
      781 caactcggca tgagcatcac cctgcccagc aaagcggcct gcgccgccgg tgccccccag
      841 ccgcctaccc cagccgctgc ccagtctact cccaccgccc ggcccacagc cgctcccgcc
      901 cggcccaccg cgccgccgac cacggcgccg gccacaggca ccccgccgcc ggccacgccc
      961 acctcgccgc cgactgccgg cgcgtccgtc tatgtcatcc ggtccggtga cacgctgtcc
     1021 agcatcgcct ggcgcgtcta cggcaacccc ggcgcctggc cctgcatctg ggcggccaac
     1081 cagtggctca ccaacccgcg cttcctgctg cgcggctggc agatcactct cccgcccgcg
     1141 gccacgtgcg acccgcccgc tcccacgcct gcggctaaag cgaccgccgc gcccgccgcc
     1201 cgttaccatg tggtggtcag catggagacg ctgtcgggta tcgcctgccg ctactacacc
     1261 gactgcaact actggcgcat ctataacgcc aaccgcgaca agatctggaa tgtaaacttt
     1321 atcctgcccg gcaccgtgct gcgcatcccg taaggcgcgg cccgccattc acaaaaccgt
     1381 cacccatcgc taccagccct ctcactcacg ggggccataa tatgctgtga gagcgggttt
     1441 ctgagccttc cacgcagtcc ctactctccg tctgcactca gcactcagca ctcagcactc
     1501 agcactcgat ttgccacgca agccgtggct ctggtacaat gcgctatgtc cacggtcttg
     1561 cggctcttgc gctatctcct ggggatcgtc gtcctcgttt gtgtgatcgc cgccggcatc
     1621 acgatctacg cgttcaccga cctgaaccgg gcggcggggc cggcggcggc accgcagcaa
     1681 ctattcacca tcgaccaggg cgagagcgtg gcctcgattg ccgcgcgcct ggagcagcat
     1741 gggttgatca attcggccac cctgttccgc atcgatctca agctgcgcgg cgccgaggag
     1801 cgcatcaaag ccggcgattt ccagttgtcc gccgggatga cgccgcagca gatcatcgag
     1861 gccatcacca cggcgcccag cgccaccatg gtccaggtgc tgatccccga aggctggcgc
     1921 atcggccaga tcgccgacaa gctggccgag ttggacctgg tggaccgcga ccgcttcatc
     1981 tcgctgacca tgaccggcac gtttcagtac gactttctgc cggaccggcc cgcgggcggg
     2041 gagatcgagg gttacctgtt tcccgacacc tacggctttc cgcgccacgg taccgaccgc
     2101 gaacagcgcg tgctcgacat catgctgacc aacttcggca agcgagtgac gccggagatg
     2161 cgcaaggacg ccacggccac gggctacacg ctccaccaga tcgtcaccct ggcctcgatt
     2221 gtcgagcgcg aagcccagtt ggattccgag cgggcgcgca tcgccggggt gttctataac
     2281 cggctcaagg cgggcatgaa gctcgacgcc gacccgacca cgcagtatgc gct
//
LOCUS       DASUYC010000411         2033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_50787,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000411 DASUYC010000000
VERSION     DASUYC010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50787"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="VF276_06785"
     CDS             complement(<1..499)
                     /locus_tag="VF276_06785"
                     /inference="COORDINATES: protein motif:HMM:NF037273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606606.1"
                     /translation="MPDPIPTPLSRESLGHLAYLHRVLAQPPGRWDGFYTPQADAMNF
                     GLRFQLAFAAYAVAALGLHTPAYTRPYVEALGGALQRMLDVRAWGYWRPAPAPAAGGA
                     RPAGHVAVLLAPHGRAAGPLPPPADPVVQDNVQFSGHLGTMLGLFERAGGDDRYDRGF
                     RLADPA"
     gene            706..1176
                     /locus_tag="VF276_06790"
     CDS             706..1176
                     /locus_tag="VF276_06790"
                     /inference="COORDINATES: protein motif:HMM:NF021357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HEX6606607.1"
                     /translation="MQWVTRKGVKLDRSACAWLVLRHLDPQAEFLYLEAEPMQEAIAA
                     GAKPFHNVTYAGPGSRTRSSFEELLAENGLDQRDPALAEMATFVHNAEIKGEAAGADD
                     ALRAIAKGVNAQVRSDQEMVERMLPIFDALYSYCRRKAAGQTRWAGLEPGWGQG"
     gene            1693..>2033
                     /locus_tag="VF276_06795"
     CDS             1693..>2033
                     /locus_tag="VF276_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606608.1"
                     /translation="MANRKHWWLTALLLLMLVGTTLPAAAGNTGQADSRTFPETGKTV
                     SGKFLAYWNSHGGLAQLGLPLTDQVQEVSPTDGKTYAMQYFERMVLELHPENAGTLFE
                     VQGSLLGYFYYL"
BASE COUNT          379 a          621 c          697 g          336 t
ORIGIN      
        1 tcgccgggtc ggccaggcgg aagccgcggt cgtagcggtc gtcaccgccc gcgcgctcga
       61 acaggccgag catggtgccc aggtgcccgc tgaactggac gttgtcctgc accacggggt
      121 cggcgggcgg gggcagcggg ccggcggcgc ggccatgcgg cgccagcagc acggcgacat
      181 ggccggcggg gcgcgctccc ccggcggcgg gggcgggtgc cgggcgccag tagccccagg
      241 cgcgcacgtc aagcatccgt tgcagggcgc cgcccagcgc ctcgacatag ggccgcgtat
      301 aggctggggt atgcagcccc agcgcagcca cggcgtaggc tgcaaaggcc agttggaagc
      361 gcaggccgaa gttcatggcg tcggcctgcg gggtgtagaa gccgtcccac cggccgggcg
      421 gctgcgccag cacccggtgc aggtaggcca ggtggcccag cgactcgcgg ctcaacggcg
      481 tggggatcgg atcgggcatg gcggttcctc acagatgcga atattctgtc aagataacgg
      541 gcgcgagcgg cgatagtgac ccgccctgag ccacgggcag gtttgaaacc ggcccctaca
      601 gtgggcggtg cgccgtggcg gcgggttgcc gggtgggcgt ggatcgctat aatggggcag
      661 aacagagtcc acgctattgc tttagcgacg aaggaggcaa ttagtatgca gtgggtaacg
      721 cgcaaagggg tgaaactaga tcggtcggcg tgcgcctggt tggtgctgcg gcatcttgat
      781 ccacaggcgg agttcctcta cctggaggcc gagccgatgc aggaggccat cgcggcgggg
      841 gcgaagccct tccataacgt gacctacgcc gggccggggt cgcgcacccg cagcagcttc
      901 gaggagttgc tggccgaaaa cgggctggac cagcgcgacc cggcgctggc cgagatggcg
      961 acgtttgtcc acaacgccga gatcaagggc gaagccgccg gggccgacga cgccttgcgg
     1021 gccatcgcca agggtgtgaa cgctcaggtg cgcagcgacc aggagatggt cgaacggatg
     1081 ctgccgattt tcgacgccct ctacagctat tgccggcgca aagccgcggg ccagacgcgc
     1141 tgggcagggc tggagccggg ctggggccag ggctagcggg cgcagcgcca accgagcgaa
     1201 cgggccggtg cccggcggcg cgccgccggg caccggtatg tctgcgcagg ccgcccccct
     1261 ctcggtaagc ccgccggctg cttacacgaa aagaataatt cttgaagcaa gagaatattc
     1321 taatttgttt ctatgtgcta atgtagaaca tacatatagt gcatagaaat tacaacatat
     1381 cttcaattat ctgtagcaaa tggggttttg cttgacagtc tacaaaaggc aatgctataa
     1441 tgtgcccgtc tcgacagcca ggatcatgct gtagttcggt tagactggtg ttcttgatca
     1501 gttcaactac aacggcacat ccagtcctcg caacatccgc cctcagcgtg aagtagatcg
     1561 agacagttcc acgcgacccg taccccgcac ctgcccctgg ccgggcctgc cggtatccag
     1621 cggatgcgga cgcgggtcca gacactaccc ttcgcggctg cattgtcttg tgtactgtaa
     1681 ggaggacctt tcgtggcgaa ccgcaaacac tggtggctaa cagccctgct gttgctgatg
     1741 ctggtcggaa cgacgctgcc cgccgccgcc ggcaataccg gacaggccga ctcgcgcacc
     1801 ttccccgaaa ccggcaagac ggtgtcgggc aagttcctgg cctactggaa cagccacggc
     1861 ggcctggccc aactggggct gccgctgacc gaccaggtgc aggaagtctc gccgaccgac
     1921 ggcaagacct acgcgatgca gtatttcgag cgcatggtgc tcgaactgca tcccgagaac
     1981 gcgggcacgc tctttgaggt gcaaggttcg ctgctgggtt acttctatta tct
//
LOCUS       DASUYC010000412         2562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1396193, whole genome shotgun sequence.
ACCESSION   DASUYC010000412 DASUYC010000000
VERSION     DASUYC010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1396193"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VF276_06800"
     CDS             <1..657
                     /locus_tag="VF276_06800"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEX6606609.1"
                     /translation="GLKSAYIRPLVWRGYGEIAPSPLKNPVEVMIAAWNWGAYLGEDA
                     LENGADVAVSSWTRMAPNTIPAMAKAAGNYLSSQLITMEAERNGYVEGIALDVNGQLS
                     EGAGENLFVIRDGIIFTPPITAALLPGITRDTVMTLARDMGYEVREQNLPREALYLAD
                     ELFFTGTAAEVSPIRSVDRQVIGTGKRGPITTQLQKAFFGLFNGETRDRWGWLDYLEA
                     "
     gene            840..2276
                     /gene="leuC"
                     /locus_tag="VF276_06805"
     CDS             840..2276
                     /gene="leuC"
                     /locus_tag="VF276_06805"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX6606610.1"
                     /translation="MAQTGPRTLFQKIWESHLVRTETPETPAVLYIDLHLVHEVTSPQ
                     AFTQLRERGMTVRRPEQTLATMDHSTPTTPRGADGAIAVVDMQAAAQLTQLERNCADF
                     GIPLYALGSDRQGIVHVIGPEQGFTQPGMTIVCGDSHTSTHGAFGALAFGIGTSEVAH
                     VLASQCLIQNEPRTMEIRVDGKLPPGVGAKDIILAVIAQIGVGGGTGYVFEYTGEAIR
                     GLNMEERMTICNMSIEGGARAGLIAPDETTFAYLAGRPHAPRGAAWDAAVAAWRQLPT
                     DPGATYDRTITLDAASLTPMITYGTNPGMGIPIDGRVPDPEAVTDASQRQALDKALRY
                     MNLQPGQPLLGKPVDVVFLGSCTNSRISDLRLAASLMRGRHVAPGVRMLVVPGSQSVK
                     RQAEAEGLHEVFREAGAEWREAGCSMCIAMNGDQLAPGQYAVSTSNRNFEGRQGKGGR
                     TFLASPLTAAATAITGAIADVRQLLETG"
BASE COUNT          451 a          892 c          846 g          373 t
ORIGIN      
        1 ggcctcaaga gcgcctacat ccgcccgctg gtgtggcgcg gctacggcga gatcgccccg
       61 tcgccgctca agaacccggt cgaggtgatg atcgcggcgt ggaattgggg cgcgtatctg
      121 ggcgaggacg cgctggagaa cggcgccgac gtagccgtgt cgtcctggac gcgcatggcg
      181 cccaacacga ttccggccat ggcgaaggcc gccgggaact atctctcatc gcaactgatc
      241 accatggagg ccgagcgcaa cggctacgtc gagggcatcg cgctcgacgt aaacggccag
      301 cttagcgagg gcgcgggcga gaacctgttc gtgatccgcg acggcatcat cttcaccccg
      361 ccgatcaccg ccgccctgct gcccggcatc acccgcgaca ccgtgatgac gctggcccgc
      421 gatatgggct acgaggtccg cgagcagaat ctgccgcgcg aggcgctcta cctggccgac
      481 gagttgttct tcaccggcac cgccgccgag gtctcgccga tccgctcggt ggaccgccag
      541 gtcatcggca ccggcaagcg cggccccatc accacccagc tccagaaggc gttcttcggc
      601 ctgttcaacg gcgagacgcg cgaccgctgg ggctggctcg actatctgga ggcgtagggg
      661 cggcatccgg gcaaggggac aaacctaacc cccctgcccc ccttcccgtc gcgggaaggg
      721 ggaaaagcgg actcccctcc ccgcggcggg gaggggccgg ggggagaggt tgctcgcccc
      781 accgaaatgc ccgctataaa cgtgtgaaac aagacgcaag aaggcggagg ttcgagatca
      841 tggcacagac aggtccacgc accctgtttc agaaaatctg ggagagtcac ctggttcgca
      901 cggagacgcc ggagaccccg gcagtgctct atatcgatct gcatctcgtc cacgaggtga
      961 cttccccgca ggcgttcacg cagttgcgcg agcgcgggat gaccgtgcgc cgccccgagc
     1021 agacgctggc cacgatggac cactcgacgc ccaccacgcc gcgcggcgcg gacggcgcga
     1081 ttgccgtggt cgatatgcag gccgctgccc agttgaccca actggagcgc aactgcgccg
     1141 acttcggcat cccgctctac gcgctgggca gcgaccgcca gggcatcgtc cacgtcatcg
     1201 ggccggagca gggcttcacc cagccgggca tgaccatcgt ctgcggtgac agccacacca
     1261 gcacccacgg cgcgttcggc gcgctggcct tcggcatcgg cacctcggag gtcgcccacg
     1321 tgctggcgag ccagtgcctg atccagaacg agccgcgcac gatggaaatc cgcgtggacg
     1381 gcaagctgcc acccggcgtg ggggccaagg acattatcct ggccgtgatc gcccagatcg
     1441 gggtcggcgg gggcaccggc tacgtcttcg agtacaccgg cgaggctatc cgcggcctga
     1501 acatggaaga gcggatgacg atctgcaaca tgtccatcga gggcggggcg cgcgccgggc
     1561 tgatcgcgcc cgacgagacc acgttcgcgt acctggccgg gcggccccac gcgccgcggg
     1621 gcgcggcctg ggacgcggcg gtggcggcct ggcggcaact gcccaccgat cctggcgcga
     1681 cctacgaccg caccatcacg ctggatgccg ccagcctcac gccgatgatc acctacggca
     1741 ccaaccccgg catgggcata cccatcgacg ggcgcgtgcc tgacccggaa gccgtgaccg
     1801 acgcgagcca gcggcaggcg ctcgacaagg cgctgcgcta catgaacctg caaccgggcc
     1861 agccgctgct gggcaagccg gtggacgtgg tcttcctggg tagttgcacc aactcgcgca
     1921 tctccgacct gcggctggcc gcgagcctca tgcgcgggcg gcatgtggcg ccgggcgtgc
     1981 gtatgctggt cgtgcccggc tcgcaatcgg tgaagcgcca ggccgaggcc gaggggctgc
     2041 acgaggtctt ccgcgaggcg ggcgccgagt ggcgcgaggc gggctgctcg atgtgcatcg
     2101 ccatgaacgg cgatcagctt gcgcccggcc agtacgccgt ctcgaccagc aaccgcaact
     2161 tcgagggccg ccagggcaag ggcggacgca ccttcctggc aagcccgctc accgcggcgg
     2221 ccacggccat caccggcgcc atcgccgacg ttcgccaatt gctggagacg ggatgatctc
     2281 tcgccgtggc tcgacccgcc gcccttgtca ctctgagcgc agcgaagagt ctcgtctcgc
     2341 cacgccttgg cactctgagc gcagcgaaga gtctcgtctt gtcccccaac gagacgagat
     2401 gcttcacttc gttcagcatg acaacccaag agtgtcactc tgagcgcagc gaagagtctc
     2461 gtcttgtccc ccaacgagac gagatgcttc acttcgttca gcatgacaac aaaattggag
     2521 catagcatgg accctatcac cacgtttacg gccggcatcg tc
//
LOCUS       DASUYC010000413         2050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_928471,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000413 DASUYC010000000
VERSION     DASUYC010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_928471"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(439..1464)
                     /locus_tag="VF276_06810"
     CDS             complement(439..1464)
                     /locus_tag="VF276_06810"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6606611.1"
                     /translation="MPQAGTILLAELDSVSTQLLLFILQRAGFEVLTATGGEDLVRQA
                     RRRRVDLIVSEVLLPDADGLDVCRQLREDWHTRFIPIVLLTAFSDAEYRVGGLLAGAD
                     DYITKPFDSNELVLRLRRLIETYRACFQYHPLTRLPGVTLIQAYLSTVCLHPQAGPWA
                     YLHVDLNHFRAFNQLYGYEAGDAVLRMTGDLLREVVFAAPGALRFAGHRSADDFALVV
                     PGDEADTVCRALISRFDACVLDRYPPEHRETCYQMLIDRRGNVLSVPRLALSIGVVTS
                     DLCRDISCLELEEVGWAVMQRAKREEQSSSYVNRRQVAGRRAAQREQPSLETRMPGAE
                     APVHEAS"
     gene            complement(1779..>2050)
                     /locus_tag="VF276_06815"
     CDS             complement(1779..>2050)
                     /locus_tag="VF276_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEX6606612.1"
                     /translation="GVFNFLTGRVRRAPEWMQRSGMEWLYRVSQEPRRLWRRYFVQDM
                     PIMMQMAAEAAQVRAFGPAQPAPHGLNPVAVEVAPARVLPRNLPN"
BASE COUNT          362 a          660 c          734 g          294 t
ORIGIN      
        1 aaatgaagat gccgaagaag gtggtgaacg agacagcggt ggtcacatcg cccaggccga
       61 gatcgcccag tttcgccggc aggaccagca cgcgcaggat attgagcacg aagccaaacc
      121 aactggaacc gagcacgatc atgacggtgc gcagcgttcc cggcccacga ttcataaacg
      181 atcctccggc ctgaaggcga gtgaacggtg cctcgggggc accggcaacc gcctcgcggt
      241 attctagatg aaagcgaccg gcggggcagc gggcccggtg gccggtctgg gttggcaagt
      301 ggagaggggg cgaccccgcg ccggtctgtc cggcgccgcg caggggacga tggctcccta
      361 catcgtctat tgtagggagc ggcggtgaac ggctaatgaa tgttctacag gcggcagtct
      421 tacaaaatcg ctattatttc agctcgcctc gtggacgggc gcctcagcgc cgggcatccg
      481 ggtctccagc gagggctgtt cacgttgcgc ggcccggcgc ccggccacct ggcggcggtt
      541 cacatagctg ctgctctgtt cctcgcgctt ggcgcgctgc atcaccgccc agccgacttc
      601 ttccagttcc aggcaactaa tatcgcggca gaggtcgctg gtgacgacgc cgatggacaa
      661 ggccaggcgc ggcacggaca ggacgttgcc gcgccggtcg atcagcatct ggtagcaggt
      721 ctcgcggtgc tccgggggat agcggtcgag cacgcaagcg tcgaagcggc tgatcagcgc
      781 ccggcagacg gtatcggcct cgtcgcccgg cacgaccagc gcgaagtcgt cggcgctgcg
      841 atggccggcg aagcgcaagg cgcccggcgc cgcaaacacc acctcgcgca acaggtcgcc
      901 ggtcatgcgc agcacggcgt cgccggcctc gtagccgtaa agctggttga acgcgcggaa
      961 gtggttgagg tccacatgca ggtaggccca gggcccggcc tgcggatgca ggcagaccgt
     1021 gctgaggtag gcctggatca gggtgacgcc cggcaggcgg gtcagcggat ggtactggaa
     1081 gcaggcgcga taggtttcga tcagccggcg caggcgcagg accagctcgt tggagtcgaa
     1141 gggcttggtg atatagtcgt cggccccggc cagcaagccg cccacgcggt actccgcatc
     1201 ggagaacgcc gtcagcagca cgatggggat gaagcgggtg tgccagtcct cgcgcagttg
     1261 ccggcatacg tccaggccgt cggcatcggg caagagcacc tcggagacga tcagatcgac
     1321 gcgccgccgg cgggcctggc ggacgagatc ctcgcccccg gtagcggtca gcacctcaaa
     1381 gccggcgcgc tgcaagatga acagcagcaa ctgggtggag acgctatcga gttccgccaa
     1441 cagaatggtg ccggcctgtg gcatacagat aaccttatct cagagcctgt aaacgacgaa
     1501 ggccggagag tccggccccg cgcggtgggt cagggggagc gcgtcccctc tgatccgccg
     1561 gtatgtggtg tatccccagg aaccgtaacg gtgtgcgccg cccggcggac ccgcctgacg
     1621 ccgacgagcg cgacctgcgg gcggctacgc ccaagagttg gctgttgggc ggaaccgcgc
     1681 gtaggtcgcg ctcggtgccg aacgggaccc cgcgcccggc gggggccgtc cccaccaggg
     1741 gagggcgtcc cgcgcgggca ggtgcgcgtt gagctggact agttgggcag gttgcgcggc
     1801 agcacgcggg ccggcgcgac ttcgacggcg accgggttca agccgtgcgg ggcgggctgc
     1861 gccgggccga acgcgcggac ctgcgcggcc tccgccgcca tctgcatcat gatcggcatg
     1921 tcctgaacga agtagcggcg ccacagccgg cgcggttcct ggctcacgcg atagagccat
     1981 tccatcccgg agcgctgcat ccactccggc gcccggcgca cccgcccggt caggaagttg
     2041 aacacgccgc
//
LOCUS       DASUYC010000414         3049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_2017503, whole genome shotgun sequence.
ACCESSION   DASUYC010000414 DASUYC010000000
VERSION     DASUYC010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2017503"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VF276_06820"
     CDS             complement(<1..347)
                     /locus_tag="VF276_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEX6606613.1"
                     /translation="MADTRVTHPPPGSDPLWQQDFPISRYEELNNSRRQFLKFLGLTS
                     IAFFTGTLATALKALFESRRTTTLAPLRIAAVADVPEGSSLNFHYPDQSYPAVLIHLP
                     GNQYVAYESRCTHL"
     gene            complement(349..960)
                     /locus_tag="VF276_06825"
     CDS             complement(349..960)
                     /locus_tag="VF276_06825"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX6606614.1"
                     /translation="MIFIDPLDTDWSTQTSPTVCMHCVNPPCANVCPADAIKVDERGV
                     VMSALPDRCIYCRNCLYACPFGIPKYQAKQALMMKCDQCYDRSSQGLKPWCATVCPTK
                     ALDYGEYDEIVAQHPGQAINQWQFGDQVLETNVYVFGPPQTKKYEILHLFDYPAQAGY
                     APNVGLAGGQQEIPGESATDVWAGWEQRDWEGWSALEGFKEEK"
     gene            complement(1135..>3049)
                     /locus_tag="VF276_06830"
     CDS             complement(1135..>3049)
                     /locus_tag="VF276_06830"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HEX6606615.1"
                     /translation="LDFIVRRYREIQAQHGQNAVAIYSGSSMTTEKSYLMGKFARVAV
                     GTGNIDYNGRLCMVSAAYANNMAFGVDRAANPWSDMALADVIFSAGSNTAECHPLSMP
                     YMWEARDRGAKIIVVDPRVTPSARTADVHLQIRPGTDTALGNAILQQMIQHDWLDHDF
                     INTRTVGFEETAALVAAYTPQKVATLCGIPAARIEEAAELWGTAKTSFLTHARGIEHS
                     TNGTNNVLSYINIVLASGRIGRPGCGYGTLTGQGNGQGGREHGQKADQLPGQRKYDDP
                     EARAYIAPIWGIKESEFPPKGLAITDMIEPMIRGEIRAMINICSNPMVSLPDQAAIRK
                     ALETLELYVVIDFFMSESAQLADIVLPGSVWAEDEGTTTNVEGRVIRINKAANPPGEA
                     RVDWDIICDLARRFGHGAQFNYHSAGEIWDELRLASKGGKADYYGITYEKIVEQDGVF
                     WPCPTLESKGTPRLFEDKFYHPDGKARFHALPYKPPAELPDAEYPFFYTTGRVIFHYL
                     SGNQTRRIGALVENAPEPYVEIHPLAAEKFGVDSGDLLRVRSRRGEMILPAKVTRTIR
                     PDTVFIPYHWGGGQAANNLTIRAYDPISRIPEFKVCAVWVEKAPEGATVTRDAMRELP
                     LSPPRALPGEQFA"
BASE COUNT          460 a          974 c         1044 g          571 t
ORIGIN      
        1 aggtgcgtgc agcgcgactc gtaggccacg tactggttgc ccggcaggtg gatcaacacg
       61 gcggggtagc tttgatcggg ataatggaag ttgagcgagc taccctcggg cacgtcggcc
      121 acggcggcga tgcgcagcgg cgccagcgtg gtcgtgcgcc ggctctcgaa cagcgccttc
      181 agcgccgttg ccagcgtgcc ggtgaagaag gcaatcgagg tcagccccag gaacttgagg
      241 aactgccgcc ggctgttgtt caactcctca tagcggctga tcggaaagtc ttgctgccac
      301 agcgggtcgc tgccgggcgg cggatgcgtg acgcgggtat cggccacctt acttctcctc
      361 cttgaaccct tccagcgcgc tccagccttc ccagtcgcgc tgttcccacc cggcccacac
      421 atcagtggcg ctctcgccgg ggatctcctg ctggccgccg gccaggccca cgttcggggc
      481 gtagccggcc tgggccggat agtcgaacag gtgcaagatc tcgtacttct tggtctgcgg
      541 cggcccgaac acgtagacgt tggtttcgag cacctggtcg ccgaactgcc actggttgat
      601 ggcctggccg gggtgctggg ccacgatctc gtcgtattcg ccgtagtcga gcgccttggt
      661 cgggcacacc gtggcgcacc acggcttcag gccctggctg ctgcggtcat agcactggtc
      721 gcacttcatc atcagcgcct gcttggcctg gtacttgggg atgccgaacg ggcaggcgta
      781 aaggcagttg cggcaataga tgcagcggtc gggcagggcg ctcatcacca cgccgcgctc
      841 gtccaccttg atggcatccg ccggacagac gttggcgcag ggcgggttga cgcaatgcat
      901 acagacagtc gggctggttt gcgtcgacca gtccgtatcc agcgggtcaa tgaagatcat
      961 cgagactccg cggtgcgtgt cgcactcctg gcaggccgcc atgcacgagc gacagccgat
     1021 gcaacgctgc ggatccatga agaattttct agccaatgtg tgctcctttg tgcgagggat
     1081 ggctggtggc ggcgcggcgg cccggcctcg cgccgccacc actcgtcacc acggttaagc
     1141 gaactgctcg cccggcaagg cccgcggcgg cgacagcggc agctcgcgca tcgcgtcgcg
     1201 ggtcacggtg gccccttccg gcgccttttc gacccacacg gcgcagacct tgaactccgg
     1261 gatgcggctg atcgggtcgt aagcgcggat ggtgaggttg ttcgcggcct ggccgccgcc
     1321 ccagtgatac gggatgaaca ccgtgtcggg ccggatcgtc cgcgtaacct tagcggggag
     1381 gatcatctcg ccgcgccggc tgcgcacccg cagcaggtcg ccgctgtcca cgccgaactt
     1441 ctcggcggcc agcgggtgga tctccacata cggctccggc gcgttctcca ccagggcccc
     1501 gatgcggcgc gtctggttgc cgctgagata atggaagatc acccgcccgg tcgtgtagaa
     1561 gaacgggtac tcggcgtcgg gcaactcggc cggcggcttg tagggcagcg catggaagcg
     1621 cgccttgccg tcggggtgat agaacttgtc ctcgaacagg cgcggtgtgc ctttgctctc
     1681 cagggtcggg cagggccaga acacgccgtc ttgctcgacg atcttctcgt aggtgatgcc
     1741 gtaatagtcg gccttgccgc ccttggaggc caggcgcaac tcgtcccaga tctcgcccgc
     1801 cgagtgatag ttgaactggg cgccgtggcc aaagcgccgc gccaggtcgc agatgatgtc
     1861 ccagtccacg cgtgcctcgc cgggcgggtt ggccgccttg ttgatgcgga tgacccggcc
     1921 ctcgacgttg gtcgtcgtgc cctcgtcctc ggcccacacg ctgcccggca gtacgatatc
     1981 cgccagttgc gcgctctcgg acataaagaa gtcgatcacg acatagagtt ccagcgtctc
     2041 cagggccttg cggatcgccg cctggtcggg cagcgagacc atcgggttgg agcagatgtt
     2101 gatcatggcg cggatctcgc cgcggatcat cggctcgatc atgtcggtga tcgccagtcc
     2161 cttcggcggg aactcggact ccttgatgcc ccagatcggc gcgatatagg cgcgcgcctc
     2221 ggggtcgtcg tacttgcgtt ggccgggcag ttggtcggct ttctgcccgt gctcgcgccc
     2281 gccctggccg ttgccctgac cggtcagcgt gccgtagccg cagccgggcc gcccgatgcg
     2341 cccgctcgcc agcacaatgt tgatgtagga cagcacgttg ttggtgccgt tggtcgagtg
     2401 ctcgatgccg cgggcatggg tcaggaagct ggttttcgcg gtgccccaga gttccgccgc
     2461 ttcctcgatg cgcgccgccg gaatgccgca gagcgtagcg accttttgcg gcgtataggc
     2521 ggcgaccagg gccgccgtct cctcaaagcc cacggtgcgc gtgttgatga agtcgtggtc
     2581 gagccagtca tgctggatca tctgctgcag gatggcattg ccgagcgccg tatcggtgcc
     2641 ggggcggatc tgcaggtgga catcggcggt gcgcgcgctg ggcgtgaccc gcgggtcgac
     2701 gacgatgatt ttggcgcccc ggtcgcgcgc ctcccacata tagggcatgc tcagggggtg
     2761 gcattcggcg gtgttgctgc cggcggagaa gatcacgtcg gccagggcca tgtcgctcca
     2821 gggattggcg gcgcggtcca cgccgaaggc catgttgttg gcgtaggccg cgctgaccat
     2881 gcacaggcgc ccattgtagt cgatgttgcc ggtgccgacg gccacgcggg caaacttgcc
     2941 catcaggtac gacttctcgg tggtcatcga actgccggaa tagatcgcca cggcgttctg
     3001 gccgtgctgg gcctggatct cgcggtagcg ccgcacgata aagtcgagc
//
LOCUS       DASUYC010000415         2570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1952814, whole genome shotgun sequence.
ACCESSION   DASUYC010000415 DASUYC010000000
VERSION     DASUYC010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1952814"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..2451
                     /locus_tag="VF276_06835"
     CDS             25..2451
                     /locus_tag="VF276_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX6606616.1"
                     /translation="MHDEPQQPVEPDGSGSASGATGGATRQTVRVARPSPNAGISTAP
                     AAVAAPPATEIGPSVPVQKPGEALTAAGARLSMAELETHQVRRGVRPGDKYVRRVRPT
                     DEGFRRVGPGHLEALPRTLEPTTGVGRLFAVLKRIIIGTPLSTAAAAHERLNKVQALA
                     VLSSDALSSVAYATEAVLGSLLVAGYAAFQANITISIAIAVLIAIVIFSYRQTIYAYP
                     KGGGSYIVAKDNLGTLPGLVAAGSLLTDYTLTVAVSISAGVFAVISLAPALAQHRVAA
                     CLVAIAVILLLNLRGLREAGNVFSIPTYVFLATVFLMLGLGLFEFFTGSLSSVANVQN
                     VIPPGNPRTGQLETVGLVLLLTAFSSGCTAMTGIEAISDGVPAFKAPESRNAAQTLVT
                     LGVLLSVMFLGISFLADQMSAKPSGTESVLSQVGRTVFAPLHLTAGGQDVPWFILQWA
                     TALILVLAANTSFSDFPRLSFFLARDRFLPHQFGFRGDRLAYSFGIVTLALLAAILII
                     VFGGDTGALLPLYAIGVFSSFTLSQSGMVARWMRLKTPGWQRYALINGIGALTTFLVL
                     LVLAWTKFAEGQPLFTIGGFTVHAGAWIVLVIVPLLMAMFLRINRHYALVARALSLQN
                     VDPQVGKAAAAGATLASSPSINLPNAGTQAGIVAPQGDEFQRIEHLVVMPVAGVNQVT
                     LRTLAYARSITDNVVAVHVAADEDAAEVEKLEAKWHRWVPDVPLVIVESPYRSLLRPL
                     LSYIDALHRQQPSRILTVLIPEFVATRPWEQLLHNQSALRLKGSLLFRPGIVVTSVPY
                     HIDTRMEALD"
BASE COUNT          371 a          885 c          849 g          465 t
ORIGIN      
        1 tctagaaaag gtgagttcgt gagcatgcat gacgaaccgc aacagcccgt ggagccggat
       61 ggatcggggt ccgcctccgg cgccacgggc ggcgcgacgc ggcagacagt ccgtgtggcg
      121 cgtccctccc ctaatgccgg catcagtacg gcgcccgccg cagtggcagc gccgccggcg
      181 accgaaatcg gcccatcggt gccggtccag aagcccggcg aagccctgac agcagcgggc
      241 gcgcgcctgt cgatggccga actggagacc caccaggtgc gccggggcgt gcgccccggc
      301 gacaaatatg tgcgccgggt ccgcccgacg gacgagggat tccgccgggt ggggccgggc
      361 cacctggagg ccctgccgcg cacactggag ccgaccaccg gcgtcgggcg gctgttcgcc
      421 gtcctcaaac ggatcattat cggcaccccg ctcagcaccg ccgccgccgc ccatgagcgc
      481 ctgaacaagg tgcaggcgct ggccgtgctg tcgtccgatg cgctctcgtc cgtggcctac
      541 gcgacggagg cggtgctggg cagcctgctc gtcgccggct atgcggcttt ccaggccaat
      601 atcacgatct ccatcgccat cgccgtgctg atcgccatcg tcattttctc ctaccggcag
      661 acgatctatg cctatcccaa gggcggcggc tcgtacatcg tcgccaagga caatctcggc
      721 acgttgccgg ggctggtggc cgcgggatcg ctgctgaccg actatacgct cacggtggcg
      781 gtgagtatct cggcgggcgt cttcgccgtc atctcgctgg cccccgcgct ggcccagcac
      841 cgggtggcgg cctgcctagt ggcgattgcc gtcatcctgc tgctgaacct gcgcggcttg
      901 cgcgaggcgg gcaacgtctt ctctatcccg acctatgtct tcctggcgac tgtgtttttg
      961 atgctggggc tggggctttt cgagttcttc accggcagtc tgagcagcgt cgcgaatgtg
     1021 cagaacgtca tcccgcccgg taatccacgg accggccagt tggaaacggt gggcctggtc
     1081 ctgttgctca ccgcgttcag cagcggctgc acggccatga ccgggatcga ggccatttcg
     1141 gacggcgtgc cggcgttcaa ggcgccggag tcgcgcaacg cggcgcagac gctggtcacg
     1201 ctgggggtgc tgctctcggt catgttcctg ggcatctcgt tcctggccga tcagatgagc
     1261 gcgaaaccgt cgggcactga gagcgtgctc tcgcaggtcg ggcgcacggt attcgccccg
     1321 ctgcacctta ccgccggcgg gcaggacgtg ccgtggttca tcttgcagtg ggccacggcg
     1381 ttgatcctgg tgctggcggc gaataccagc ttttccgact tcccgcggct cagcttcttc
     1441 ctggcgcgcg accgcttctt gccgcaccag ttcggcttcc gcggcgaccg gctcgcctac
     1501 agcttcggca tcgtcacgct ggcgctgctg gcggcgatcc tgatcatcgt cttcggcggc
     1561 gataccgggg ccttgctgcc gctctacgcc atcggcgtgt ttagctcgtt caccctctcg
     1621 caaagcggca tggtggcgcg ctggatgcgc ttgaagacgc cgggctggca gcgttacgcg
     1681 ctgattaacg gcatcggcgc gctgaccacc ttcctggtgc tgctggtgct ggcctggacc
     1741 aagttcgccg agggccagcc gctgttcacc atcggcggct tcacggtcca tgccggcgcg
     1801 tggatcgtgc tggtgatcgt gcctctgctg atggcgatgt tcctgcgcat caaccgccac
     1861 tacgcgctgg tggcgcgggc gctgagcctg cagaatgtcg acccgcaggt gggcaaggcg
     1921 gcggcggcgg gtgccacgct ggcttccagc ccctcgatca acctgcccaa cgccggcacc
     1981 caggccggca tcgtcgcccc ccagggcgac gagtttcagc gtatcgaaca cctggtggtc
     2041 atgcccgtcg ccggggtgaa ccaggtcacg ctgcgcacgc tggcctacgc ccgatctatc
     2101 accgacaacg tggtggccgt gcacgtggcg gcggacgaag acgccgccga ggtggagaaa
     2161 ctggaggcca agtggcaccg ctgggtgccc gatgtgccct tggtcatcgt cgaatcgccc
     2221 taccgctcgc tcttgcgccc cttgctgtcg tatatcgatg ccctgcaccg ccagcagccg
     2281 agccggatct tgaccgtgct gatcccggaa ttcgtcgcca cgcgcccctg ggaacaactc
     2341 ttgcacaacc agtcggcgct gcggctgaag gggtcgctgc tgttccgccc cggcatcgtc
     2401 gtcacgagcg tgccctatca catcgatacc cgcatggagg cgctggattg atctgtccga
     2461 attgcgaagc gaaaaacagc cgcgcggata atttctgctg gcgctgcggc accccgctca
     2521 agaccgaggc cgccgccgcg cgtgccacaa cgccgtccgc gacgccgggc
//
LOCUS       DASUYC010000416         2004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_692912,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000416 DASUYC010000000
VERSION     DASUYC010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692912"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VF276_06840"
     CDS             <1..198
                     /locus_tag="VF276_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606617.1"
                     /translation="GAPQAESAVNLLLHTLEVPTYTIQQPGMPRTGAGFDLALPALVL
                     VLAAGALALGTGLRRRATHRA"
     gene            complement(325..1248)
                     /locus_tag="VF276_06845"
     CDS             complement(325..1248)
                     /locus_tag="VF276_06845"
                     /inference="COORDINATES: protein motif:HMM:NF019632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEX6606618.1"
                     /translation="MTMAQRIAANEALVGKYTLVPAGELSAIIDRIQRFRTAHRQEAE
                     HGSVYSHRADVADLDLEQLRAVLRSLHPGVATVIVVWPADRLAARLQYADFVACYDDL
                     WHPASTDVWITDQAVDWLVELDHEETLTVLREDPETESPLTKAALLNRLHTEHARLTQ
                     SMGLLTEAQLTQPGIIGTWSVKDMLPHIMHWEQFALAELEPAMCGERPAAPADGSGDA
                     ESEDEINARVVAQYRDVPLEAVRAAFDRSFERVVAVVEALGEAEFAPSSPVAQALGEP
                     VVDCLGNNTYEHYAEHSAMIRDWLARHKAAG"
     gene            complement(1328..>2004)
                     /locus_tag="VF276_06850"
     CDS             complement(1328..>2004)
                     /locus_tag="VF276_06850"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HEX6606619.1"
                     /translation="LALDDNALLAARDSARSALYLRTGALDRLGVKEIRFLLNDRLAR
                     HNWLSAGYQFVDGTSVAAPVVASVAVQMIEANPSLTPAAIKDLLTATAVPMQDVPPEK
                     QGAGVVNPAAAVARALRAPGGPLVGYPASPAREPDGGITFYYYNPRVRQVGLVGDFNG
                     WFAAGYRFADLGPGLFRFHLSPLPPGYYRYKFLLDDSTAVDDPESLDKEPDGYGGFNS
                     CLAVAP"
BASE COUNT          331 a          678 c          643 g          352 t
ORIGIN      
        1 ggcgcgccgc aggccgagag cgcagtgaac ctgctgctgc acacgctgga ggtgccgacg
       61 tacaccatcc agcagcccgg catgccgcgc accggcgcgg gcttcgacct ggcgctgccg
      121 gcgctcgtgc tggtgctcgc cgcgggcgcg ctggcgctcg gcacgggcct gcgccgccgc
      181 gccacgcacc gcgcataacc gtctgtcaga ggtaaagcga agcggagccg ccgtcctggt
      241 ggctccgctt ctttgattcg agtcccggcg aagtggtgta ggggcgggtt tcaaacccgc
      301 cttgccccag ctccgcgttg ctgtctatcc cgcggccttg tgccgggcca accaatcgcg
      361 gatcatagca ctatgctcag cgtagtgctc ataggtgttg ttgcccagac agtccacgac
      421 cggctcgccg agggcctgcg cgaccgggct gctcggcgca aactcggctt cgcccagggc
      481 ttcgaccacc gccaccaccc gctcgaacga ccgatcaaac gcggcccgca ccgcctccag
      541 cggcacgtca cggtattgcg ccacgacccg cgcgttgatc tcgtcctccg attccgcatc
      601 tccgctgccg tcagccgggg ccgccgggcg ttctccgcac atggccggtt ccagttcggc
      661 cagcgcaaac tgctcccagt gcataatgtg gggcaacatg tccttcaccg accatgtccc
      721 gatgataccc ggctgcgtca gttgcgcttc cgtcaacagg cccatagact gagtgaggcg
      781 ggcgtgttcg gtgtgcagcc ggttgagcag cgcggctttg gtcaaaggtg attctgtctc
      841 agggtcttcg cgcagcactg tcagcgtttc ctcgtgatct aactccacaa gccagtccac
      901 ggcttgatca gtaatccaga catccgtgct tgccgggtgc cataaatcat cgtagcacgc
      961 cacgaagtcg gcatattgca gccgggcagc aaggcgatca gccggccaaa cgacgattac
     1021 tgtcgccacg cccggatgga gtgagcggag taccgctcgc agttgttcca gatccagatc
     1081 tgctacatct gcacgatgcg aatagaccga cccgtgctca gcctcttgcc gatgtgctgt
     1141 acgaaatctt tgaatccggt ctatgattgc ggatagttcg ccagccggaa ccagagtgta
     1201 cttgcctacc agagcttcat ttgcggctat gcgctgggcc atagtcatct ttagttcctt
     1261 aagtgcagag ttgattccct gcttcttggg cacggttgcc ggacaacgcc gacagagtgt
     1321 ccgataatca gggcgcgacc gccaggcaac tgttgaatcc gccgtagccg tccggctctt
     1381 tgtccagact ctccgggtcg tccaccgcgg tcgagtcgtc cagcaggaac ttgtagcgat
     1441 aatagccggg cggtagcggg gacaggtgga agcggaagag gcccggcccc aggtccgcga
     1501 agcggtaacc cgccgcgaac cacccgttga agtcgccgac caggcccacc tgccggacgc
     1561 gcgggttata gtagtagaag gtgattccgc cgtcaggctc gcgggcgggc gacgcggggt
     1621 agcccacgag cggcccgccg ggcgcccgca gcgcgcgggc taccgccgcc gccgggttga
     1681 ccacccccgc gccctgcttc tccggcggca catcctgcat gggcacggcg gtggccgtca
     1741 gcaggtcctt gatcgccgcc ggggtcaggc tggggttggc ctcgatcatc tgcacggcca
     1801 ccgaggcgac gaccggggcg gccaccgacg tgccgtccac gaactggtag cccgccgaga
     1861 gccagttgtg ccgggccagc cggtcgttga gcaggaagcg gatctccttc acgcccaggc
     1921 ggtcgagcgc gccggtgcgc aggtagagcg cgctgcgcgc ggagtcgcgg gcggccagca
     1981 gcgcattgtc gtccagcgcc agca
//
LOCUS       DASUYC010000417         2206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1035831, whole genome shotgun sequence.
ACCESSION   DASUYC010000417 DASUYC010000000
VERSION     DASUYC010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035831"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="VF276_06855"
     CDS             <1..964
                     /locus_tag="VF276_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HEX6606620.1"
                     /translation="DDADKQAAYATLYTCLTTLARLVAPFMPYVSEALYQNLVAAQVP
                     GSPESVHLAEWPVANAALIDEALLADTRLLLDVVNLGRAARRGAGLRVRQPLPELLVR
                     AAQGGGGLHRFEDELRDELNVKTVRFLGVDDGLVEYHFKPNLPVVGKKYGRRVPALKT
                     ALAALEGDAAMDAARAVAAGQPVRVEVEGEPLDLAPNEVLVEAGSPQGYAVVEEPGLL
                     VALNTTLTPELRREGEARDLVRFIQDARKAAGLDITDRIAVTLEPAADLDLDPVLAAH
                     GAYIRAETLADSLVLGPPPAGAQTTVADLDEGTVTIGVTRSAAA"
     gene            1078..1884
                     /locus_tag="VF276_06860"
     CDS             1078..1884
                     /locus_tag="VF276_06860"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6606621.1"
                     /translation="MNPDPDSVNEESQAIWDQNAEAWDTKMGAEGGAWQQHLIAPVTE
                     RLLDVRPGEWVLDVACGNGAFARRLAHLGATVTACDFSAQLIARARSYVSPEGGRIDY
                     RVIDATNEAQLLSLGGAVFDAAVCTMALMDMTTIDPLLAALRRLLKPGGRFVFSVMHP
                     CFNTHAASKVATAVDRQGDLRTIYTMQIEQYLGLAPAKGLAIREQPALQYYFHRPLSV
                     LFNACFQAGLVMDALEEPVPAPPPGPADCFDWGANYREFPPVLVARLRLP"
     gene            2006..>2206
                     /locus_tag="VF276_06865"
     CDS             2006..>2206
                     /locus_tag="VF276_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606622.1"
                     /translation="MTTTEKPQQTVLMTRGKFAGINALADAQGVIAAAAMDQRGSLKK
                     SIAKESGKDVTDQQMEEFKTAVT"
BASE COUNT          343 a          773 c          780 g          310 t
ORIGIN      
        1 ggacgacgcc gacaagcagg ccgcctacgc cactctctac acctgcctga ccacgctggc
       61 ccggctggtc gccccattca tgccctacgt cagcgaggcg ctctatcaga acctggtcgc
      121 cgcgcaggtg cccggcagtc cggagagcgt ccacctggcc gagtggccgg tcgccaacgc
      181 ggcgctgatc gacgaggcgc tgctggccga cacccggctg ctgctcgacg tggtcaacct
      241 gggccgggcg gcgcggcggg gcgcggggct gcgcgtgcgc cagccgctgc ccgaactgct
      301 ggtgcgcgcc gcgcagggcg gcgggggcct gcaccgcttc gaggacgaac tgcgcgacga
      361 actgaatgtc aagacggtgc gcttcctggg tgtggacgat gggctggtgg agtaccactt
      421 caagccgaac ctgccggtgg tcggcaagaa atatggccgc cgcgtgcccg cgctcaagac
      481 cgcgctggcc gcgctggagg gcgatgccgc catggatgcg gcgcgcgcgg tggcggcggg
      541 gcagcccgtc cgcgtcgagg tggagggtga accgctcgac ctggccccca acgaggtgct
      601 ggtggaggcc gggtcgccgc agggctacgc cgtggtcgag gaaccggggt tgctggtcgc
      661 cctcaacacc acgctgacgc cggaactgcg ccgcgagggc gaggcgcgcg acctggtgcg
      721 ctttatccag gacgcgcgca aggccgcggg cctggacatc accgaccgca tcgccgtgac
      781 cctggagccg gcggcggacc tcgacctgga tccggtgctg gccgcgcacg gcgcctacat
      841 ccgcgccgag acgctggccg actcgctggt gctcggcccg ccgcccgcgg gcgcgcaaac
      901 caccgtcgcg gatcttgatg agggcaccgt gaccatcggc gtgacgcgga gcgccgccgc
      961 ctaaacccgc cccgcccggg ccgcccgccc ctaccggcag cccggcgcag gcctgcccgt
     1021 ccgcagccgc ccgtggaggc cggcgggata ttgttgaccc ttggaggaag ttggacgatg
     1081 aaccccgacc ccgattccgt gaacgaggag tcccaggcca tctgggacca gaacgccgag
     1141 gcatgggata cgaaaatggg cgccgagggg ggcgcctggc agcagcacct tatcgcgccc
     1201 gtgaccgagc gcctgctgga tgtacgcccc ggcgagtggg tgctcgacgt ggcgtgcggc
     1261 aacggcgcct tcgcccgccg cctggcgcac ctcggcgcga cggtcacggc ctgcgacttc
     1321 agcgcgcaac tcatcgcgcg cgcccggtcc tacgtcagcc cggagggtgg gcgcatcgac
     1381 taccgggtga tcgatgccac gaacgaggct cagttgctca gcctgggcgg ggccgtgttc
     1441 gacgccgccg tctgcacgat ggcgctcatg gacatgacga ccattgaccc gctgctggcc
     1501 gcgctgcgcc ggctgctcaa gccgggcggc cgcttcgtgt tctcggtcat gcacccctgc
     1561 ttcaacaccc atgccgcctc gaaggtggcg acggcggtgg atcgccaggg cgacctgcgg
     1621 acgatctaca cgatgcagat cgagcagtac ctggggctgg cgcccgcgaa aggcctcgcc
     1681 attcgggagc aacccgcgct gcaatactac ttccaccgcc cgctgagcgt gctcttcaac
     1741 gcctgtttcc aagcggggct ggtcatggac gcgctcgaag agccggtgcc cgcgccgccc
     1801 cccggcccgg cggactgttt cgattggggc gccaactacc gcgagttccc gcccgtgctg
     1861 gtggcccggc tgcggctgcc gtagcggtgg attgccgggg cgccgggcga atcgctggcc
     1921 cggtaggtgc tagaggatgg tgagtaacca ctgctttgcc gggcctgtcc acgtaaagga
     1981 cacgtaaagg agatgagata cctctatgac taccaccgag aaaccacagc agaccgtcct
     2041 catgacccgc ggcaagttcg cgggcattaa cgccctggcc gacgcgcagg gcgtcatcgc
     2101 cgccgccgcc atggaccagc gcggctcgct gaagaagtcc atcgccaagg agtcgggcaa
     2161 agacgtcacc gaccagcaga tggaggagtt caagacggcg gttacc
//
LOCUS       DASUYC010000418         4689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_960306,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000418 DASUYC010000000
VERSION     DASUYC010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4689
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960306"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..774)
                     /locus_tag="VF276_06870"
     CDS             complement(67..774)
                     /locus_tag="VF276_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606623.1"
                     /translation="MGALTYLFVDDQLINVQLLPAPTPAYVYVLLDQAYRGSPFINPI
                     VYQYRADGSRVGTARVPLTGPHNRVTPQLAVGDDGSIYAARVQPDGIEIRQLRVFADP
                     AQLPPVLGPPPRDLAELVAQSDAIAEGAIGPVEQIEVAGAGQWATQLLQIRRWWKSPD
                     PAPPALRLIPAPLGNPYPPRRRAPLIPRSRRRRRPPSRRAALRHQYRRRPRPRHRDIM
                     VGKRGQCRRATGLGAGG"
     gene            complement(743..1132)
                     /locus_tag="VF276_06875"
     CDS             complement(743..1132)
                     /locus_tag="VF276_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606624.1"
                     /translation="MRSLQRRNGEFWALDGVEQATAVVRLAGDGRLLARYPVPPDLAR
                     GLFNVGLEGDQAVIVQRTADPPNVQLLDAAGHLAPAALRDYLHAGRYFVGHPDDGSSR
                     GNGGSFELNLTPPATRPDGCPDLSLCR"
     gene            1174..1458
                     /locus_tag="VF276_06880"
     CDS             1174..1458
                     /locus_tag="VF276_06880"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEX6606625.1"
                     /translation="MSGYADGTFRPAANVTRAQLAKMIVQARGYPLVTPATPAFSDVP
                     VDYWAYSYIETARTHMVVGGYADGTFRPAAEATRAQLCKMLYQAGGAPAR"
     gene            2230..3078
                     /locus_tag="VF276_06885"
                     /pseudo
     CDS             2230..3078
                     /locus_tag="VF276_06885"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            3482..4273
                     /locus_tag="VF276_06890"
     CDS             3482..4273
                     /locus_tag="VF276_06890"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX6606626.1"
                     /translation="MSEPWPMLHRDARHTGVAPGVGRIPRFGPVVQKWEWRVAPPVTP
                     ATVSCYRFYSGFPLVDLDGNGTLDVVVTGPDNAIGPDCPPPGVPDLTQSTVRALRTSG
                     PGQAAALWSLAGSVYLPTAQPEWGCSDWFDQYSAVAVDTAADPIPDIVFTSKGGIVRA
                     VSGQTGALIWQYATSRFIEAGPMVADLDGDGHYEVVVPFGCRGQVRCYGDDGTKRWTY
                     QLGDPAAEWTQRSIGSPSIGDVDGDGLLDVIVSAFDGKVYCLGAP"
     gene            4412..>4689
                     /locus_tag="VF276_06895"
     CDS             4412..>4689
                     /locus_tag="VF276_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEX6606627.1"
                     /translation="MHAPDVAHDPSATTSPYLVALSDVALADIALVGGKAAGLGELLR
                     GGLPVPPGCCLTTAAYRRFIAEAGLVDPLTALAAGAAQLALPVLQQTAG"
BASE COUNT          738 a         1549 c         1600 g          802 t
ORIGIN      
        1 cggctcaatc caacactgtt gcaaaacgtg aacccgctct aacaagcgcg gcgggccacg
       61 caggccttat ccgcccgcgc ccaggccggt ggctctccgg cactgccccc gtttccccac
      121 cataatatca cggtggcgtg ggcgtggccg tctcctgtac tggtggcgca gggctgcccg
      181 ccggctcggt ggccggcgcc ggcgccgtga gcgtgggatc agcggtgccc gtcgtcgggg
      241 cgggtacgga ttgcccagcg gcgccgggat caggcggagg gccgggggcg caggatcggg
      301 gcttttccac cagcggcgga tctgtagcag ctgcgtcgcc cattgccccg ctcccgcaac
      361 ctcgatctgc tccacggggc cgattgcccc ttcagcgatg gcatcggatt gcgcgaccaa
      421 ctccgccaga tcgcgcggcg gtgggccgag caccggcggc agttgggctg ggtccgcgaa
      481 cacgcgcaat tggcggatct cgataccgtc cggctgcacc cgcgccgcat agatactccc
      541 gtcgtcccca accgccagtt gcggggtgac ccggttgtgg gggccggtga gtggcacccg
      601 cgcggtcccg acccggctcc cgtcggcccg atattgatag acgatcggat tgatgaaggg
      661 ggagccacga tacgcctggt ccaagagcac atagacatag gccggggtcg gggccggcaa
      721 gagttggacg ttgatgagtt ggtcatcgac aaagagatag gtcagggcac ccatctggcc
      781 gggttgcagg gggggtgagg ttcaactcga aggatccgcc gttgccccgg ctggagccgt
      841 cgtcggggtg gcccacgaaa tagcgcccgg catggagata gtcgcgcagc gccgcgggcg
      901 ccaggtggcc ggcggcgtcg agcagttgca cgttgggtgg gtcggcggtg cgctggacga
      961 tgacggcctg atccccttcc aggccgacgt tgaagaggcc cctggccaga tcggggggca
     1021 ccgggtagcg cgccaggagc cgcccgtccc cggccagccg cacgacggcg gtggcctgtt
     1081 ccacgccatc cagtgcccag aactccccgt tgcgccgctg gaggctgcgc acccgttcta
     1141 cacctatgtg gaagtggcgc gcagccacgg gtgttgagcg gctacgccga cggcaccttc
     1201 cgcccggccg ccaacgtcac gcgggcgcaa ctggcgaaga tgatcgtcca ggcgcgcggc
     1261 tacccgctgg tgaccccggc cacgccggcg ttcagcgatg tgccggtgga ctactgggcc
     1321 tacagctaca tcgagacggc gcggacccac atggtggtgg gcggctacgc tgacggcaca
     1381 ttccggccgg cggccgaggc gacccgcgcc caactgtgca agatgctcta ccaggcgggg
     1441 ggcgcgcccg cgcgctaagc ctgccgctgg ccgataaata cagcgccggg gcggggatct
     1501 agggtcctcc gccctggcgc tgtgttgtgc ccagcacggg gcgcttcctg tgagaggcag
     1561 caccagggcc tggggccgtg aacgtatcgg gtgatagggg cgggtggtcc aggcccggtg
     1621 ggatgcccgg cgcaagggtg tgaggcagat gcaggatctc cgcatcggag agggtgcagg
     1681 tattagtagg cattgtcatg gagttgtctc caaccaacaa aaaggcacgc cgccccggat
     1741 aagacgagac accgtgcctg aaaaccgggc gtgcgctttg tgcccttgcg ccgggcgtgc
     1801 gcttcggcta tgatcggcgt agcttgcccg ccgcttatcc cgatgggtgg gttagatctg
     1861 cgccgatgtg cccagcagcg gcgcaggccg cttttgcgcc tgcttgttgg tttgctacga
     1921 ttactctttt ggtcgggttt gttttagact cgccacttcc gcctttagcc cggccactcg
     1981 tccgctcagg ttcagcaata tcatagccgc ctcgtgcgcg atctggcctg ggccgacctg
     2041 cgctgtgtgc tccaggtggt ctgccggcgt ttcaaatgtg cggcctgccg ccggcctttt
     2101 accgagacct tggtcgccgt cgccccctat gcgcgcacca ccacgcgcta cgccgccccg
     2161 ttgcgcgcag cggtgcgcac cgccaccgtc gcgcaggttg cacgggccga acaccacggc
     2221 tttgtagccg tggaaggcat cgtctatcgc caagcccgcg caactcaccc gctcggcccg
     2281 tccacccggc gcatcacccg cctgggccgc gacgagatcg cggcgcgcaa gggccccggc
     2341 cattacaaac gggtgctcgt ggacctagag acccaccaag tggtcgacca actcgcggat
     2401 cgcgataagg cggcgctgcg cgcctactgc gggcaatggt cggcggaggc gcgggcggca
     2461 gtggcggagg tgacgaccga cttctgggcg gcctagcacg acgcggtggc agaatgactg
     2521 ccccaggccc gcgtcgtcgg ggaccggttt catgtgcaag cgcaggtcaa tgccgcgctg
     2581 aacgcgatcc accgcgacgt gcaacggcag atggggctgg cggatcaggc gtggttgcgg
     2641 cagcggcggc acttattgct gcgcaatgag gaggacctgg acgccgcgga ccaggtggac
     2701 ctggagatct tgaaggcgta tcagccgcaa ttagaggtgg cgcccacgct gaaggaacgc
     2761 ttacgcacga tctaccagac ggctccggat cgggcgagcg ctgcggcggc cttgggggac
     2821 tggcgggcgg cggtggccgg cagcggcgtg gcggcgctga tggcggtggg ggaatttgtc
     2881 gcccgctggc aggaaccgat cctcaactac tttgtgcggc gcacgacgag tgggctggtc
     2941 gaggggttga acaacaagat caagttggtg aaacggcggg cgtttgggtt cagcaatgac
     3001 gcgcactttc gcctgcgggt gctactggcg tgcgacggga ctccgtcgcc cactaatgct
     3061 caaagaaccg ataactaatc ctatcccgtc ccgatggagt cgggactatt ttccctatgc
     3121 caatggggac atcaacctca tgtccactgg gtaggctgca ggctatattt gtagtacaaa
     3181 tcgccgcacc gtgggccaag ggccagtccg cagcagagcc agcgccggtt tggtgaccgg
     3241 cctcactcgc atatccgcgc gccggccctg atcagcgccg actgccggca ccgatgccaa
     3301 tgcgcaaggt tgcgtatcgc ctgatcgttg atcgccgccc tcatcgcgct tgtcccccgc
     3361 gccgctttga catgacgata ggccgattgt ccagatagga tcctcgtgcg gggttgctct
     3421 atagcacccg ctacgccgtg ccggcgccac ggctgaaatt gattcacaag gaggcaaccc
     3481 catgagtgaa ccctggccca tgctgcatcg cgacgcccgc cataccgggg tagcgcccgg
     3541 cgtgggccgc atcccccgct tcggccctgt cgtccagaag tgggagtggc gcgtggcccc
     3601 gccggtcacc ccggccaccg tatcgtgcta ccgcttctac tccggctttc ctctcgtcga
     3661 cctcgacggc aacggcaccc tcgacgtggt cgtgaccggc ccggataacg ccatcggccc
     3721 cgactgcccg ccccccggcg tacccgacct gacccagagc accgtgcggg cgctgcggac
     3781 cagcggcccc ggccaggccg ccgccctgtg gagcttggcg ggtagtgtct atctcccgac
     3841 cgcccagccg gaatggggtt gctccgattg gttcgaccag tactccgccg tggccgtgga
     3901 cacggccgcg gatcccatcc ccgatatcgt gtttacctct aaaggcggca ttgtgcgcgc
     3961 cgtcagtggc caaaccgggg ccctgatctg gcagtacgcg acgagccgct tcatcgaggc
     4021 cggtcccatg gtggccgacc tggatggcga cgggcattac gaagtcgtgg tgccctttgg
     4081 ctgccgcggc caggtgcgct gctatggcga tgacggcact aaacgctgga cgtaccagtt
     4141 gggcgatccc gcggccgagt ggacccagcg atcgatcggc tccccatcca ttggggatgt
     4201 cgatggcgac ggcttgctcg acgtgatcgt ctcggccttc gacggcaaag tctattgcct
     4261 gggggcgccc tgatctgccc atcgaactca gggtcccgga gccgcgccgc cacagagggc
     4321 gcgcaagcgt ggtcgtacaa tagactcaga tcgtggcagt cagcacagca ctcggctccc
     4381 gaacggcgtg ccgaataggc aggagtttag tatgcatgct cccgacgtcg ctcatgaccc
     4441 aagtgcaacg acatcacctt acctggtcgc cttatcggac gtcgccttgg ccgatatagc
     4501 gctggttggg ggcaaggctg cggggctagg ggagttgttg cgcggtggac tgcccgtgcc
     4561 acctggctgc tgtctcacca ccgccgccta ccgccgcttc attgcggagg ccgggctggt
     4621 cgacccgctg accgccctgg ccgccggcgc cgctcaattg gcgttgcccg tcttgcagca
     4681 gaccgccgg
//
LOCUS       DASUYC010000419         2713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1616613, whole genome shotgun sequence.
ACCESSION   DASUYC010000419 DASUYC010000000
VERSION     DASUYC010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1616613"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            386..970
                     /locus_tag="VF276_06900"
     CDS             386..970
                     /locus_tag="VF276_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX6606628.1"
                     /translation="MIMTWNIDSAHSSVEFSAKHMMISTVRGRFTEFTGTVNLDEKDL
                     TRSSVAWTLQAASLTTGNEQRDGHLRSGDFLEAETYPTITFKSTRVERAGADRYTIYG
                     DLTIKDVTREVSFQATDEGQGKDPWGNTHWGFTVTGTLNRKEFGLNWNVALETGGVLV
                     GEQIKIALDVELVAVPAPVAAEVAEQEAVAAPVA"
     gene            1105..2034
                     /locus_tag="VF276_06905"
     CDS             1105..2034
                     /locus_tag="VF276_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010277488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6606629.1"
                     /translation="MTNFGEAAVPAVLHPDTAMGQVALTVADLDRSIRFYTQSLGFQV
                     LARDAETATLGPQGGPGLLLLTEQPGALPAPRSATGLYHFAILLPTRADLARALYRLI
                     ETQTPVQGFADHLVSEAIYLADPDGNGIEIYRDRPRAEWPRLNGQIQMATDPIDLDGL
                     MAELGPNPSAGPGLPAGTRMGHVHLQVGDIARAAAFYVDVLGFEVVTTMPSALFVSAG
                     GYHHHLGLNTWQSRGRGAPPAGRAGLRDFIVVLPDQAEQDRVVARLEAAGIPVTREGG
                     AVLAPDPWGTQVRLVVAPAATGAEPAATLAESR"
     gene            complement(2031..>2713)
                     /locus_tag="VF276_06910"
     CDS             complement(2031..>2713)
                     /locus_tag="VF276_06910"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6606630.1"
                     /translation="QDLLVGLKALVFNRTIVILAVVFAVLNLGIGAINVLWIVFLRTQ
                     FGFTAADLAWRISVVDIAFAGGMVLASVLVGNFAAHVAPKWLIAWGLIVAGAITTVMG
                     ALPTYWLLVVAMGLVGLSVGPINAGATTLMQIVVPNRQLGRVGGGLGTVMDTTLLVSM
                     GLAGVAATFLGIPIVFALAGILCAGSGALGWALLPALTLRDKVPDDGPAAGPPADVPA
                     ARDPLAVA"
BASE COUNT          486 a          938 c          919 g          370 t
ORIGIN      
        1 gggccgccca ggtcagactc agagacgggc tcgcggcacc agcgaagcac gaccttggac
       61 aagttacccg cctgccccac aggaaacgga ttttcatacg cgctggtgtg ccgtaggcac
      121 atgattggct cagagtgaca acgggcagag gcagcaccgc gcaggtaggc gcctccgtgg
      181 ccgcaccgtc ccaaccgcga cgacaccgac cccgataact tgggacccga tcccggcggg
      241 tagggcgtgg ataaggacgg gcacgaggac atttgtgggg acaaacgctt gctacactgt
      301 aaccattgaa ggcagtcccc cgataacgga ttgccccacc aggataacag cagatcggtc
      361 cggaagccgg tccagtataa ggagaatgat aatgacctgg aatattgatt ccgcccacag
      421 ctcggtggag ttttcggcga agcacatgat gatctcgacc gtgcgcggcc ggttcacgga
      481 atttaccggc acggtgaatc tggacgagaa ggacctgacc cgctcgtcgg tcgcgtggac
      541 cttgcaggcg gccagcctga ccaccggcaa cgagcagcgc gacgggcacc tgcggtccgg
      601 cgacttcctc gaagccgaaa cctacccgac gattaccttc aagagcacgc gggtggagcg
      661 tgcgggcgcg gatcgctaca cgatctacgg cgacctgacg atcaaggacg tgacccgcga
      721 ggtcagtttc caggccaccg acgaaggcca gggcaaggac ccgtggggca acacccactg
      781 gggcttcacc gtcaccggta cgctgaaccg caaagagttc ggcctcaact ggaacgtggc
      841 gctggagacc ggcggcgtgc tggtcggaga gcagatcaag atcgcgctgg acgtggagct
      901 ggtcgcggtg ccggcgccgg tggccgccga agtcgccgag caggaagcgg tcgccgcgcc
      961 cgtcgcctaa ttcgccaata cagcaactgc cgggtcgctc cggcgtggtt atccggcaag
     1021 tggaccgcgg ggcgccatcg ctccgcggtc gctttgtcgt tgactctagt gaggcccgcg
     1081 ggccgcacag caggaggttg tatcatgact aattttgggg aagccgcagt gcccgcggtg
     1141 ttacatcccg acacggcgat gggccaggtc gcgctgaccg tggccgatct cgaccgatcc
     1201 atccgcttct acacgcaaag cctgggcttc caggtcctgg cgcgcgacgc cgagacggcg
     1261 accctgggcc cgcagggcgg tcccggcctg ttgctgctga cggaacagcc gggggcgctg
     1321 cccgcgccgc gctcggccac ggggctgtat cacttcgcga ttctgctgcc cacccgcgcc
     1381 gacctggccc gcgcgctcta tcgcctgatc gagacgcaga ccccggtgca gggctttgcc
     1441 gaccacctgg tgagcgaggc gatctacctg gccgaccccg acggcaacgg gatcgagatc
     1501 taccgcgacc ggccgcgcgc cgagtggccg cgcctgaacg ggcagatcca gatggcaacc
     1561 gatcccatcg acctggacgg gctgatggcc gaactcggcc ccaatccgag cgccgggccg
     1621 ggcctgcccg ccgggacgcg tatgggccac gtccatctcc aggtgggcga catcgcgcgg
     1681 gctgccgcgt tctacgtgga cgtgctgggc ttcgaggtgg tgacgaccat gccgagcgcg
     1741 ctgttcgtgt cggcgggcgg gtatcatcat cacctgggcc tgaacacgtg gcagagccgg
     1801 ggccggggcg cgccgcccgc gggccgcgcg ggcttgcgcg acttcatcgt cgtgctgccc
     1861 gatcaggccg agcaggatcg cgtcgtggcg cggctcgaag cggcgggcat ccccgtgacc
     1921 cgcgagggcg gcgcggtgct ggcgccggac ccctggggca cgcaagtgcg cctggtcgtc
     1981 gccccggcgg ccaccggcgc ggaaccggcg gcgacgctgg ccgaaagccg ctaagcgacg
     2041 gccagcgggt cgcgagccgc gggcacgtcc gcgggcggcc ccgccgccgg cccgtcgtcc
     2101 ggcaccttgt cgcgcagcgt cagcgcgggg agcagcgccc aacccagggc gcctgacccc
     2161 gcgcagagga tgccggccag cgcgaacaca atgggaatgc cgaggaaggt ggccgctacc
     2221 ccggccaggc ccatcgacac cagcagcgtg gtatccatga ccgtgcccaa cccgccaccc
     2281 acgcggccca actgccggtt gggcaccacg atctgcatca gcgtcgtcgc cccggcattg
     2341 atcggcccga ccgacagccc cacgagcccc atggcgacca ccagcagcca gtaggtgggc
     2401 agcgcgccca tcacggtcgt aatcgccccg gccacaatca ggccccaggc gatcagccac
     2461 ttgggcgcga cgtgcgccgc gaagttcccc accagcacgc tcgccagcac catgcccccg
     2521 gcgaaggcaa tgtccaccac gctgatacgc caggccaggt cggcggcggt gaagccgaac
     2581 tgcgtgcgca ggaacacaat ccagagcacg ttgatcgctc cgatacccag gttcagcacg
     2641 gcgaacacca cggccaggat gacgatggtg cggttgaaca ccagcgcctt gaggccgacc
     2701 aggagatcct gcc
//
LOCUS       DASUYC010000420         9138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1418257, whole genome shotgun sequence.
ACCESSION   DASUYC010000420 DASUYC010000000
VERSION     DASUYC010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 9138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9138
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1418257"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VF276_06915"
     CDS             <1..577
                     /locus_tag="VF276_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HEX6606631.1"
                     /translation="LTVPEYMKEIVAELTHLARKHPDISQRSGVSVRVSVSNYENLLS
                     NAVKRAIRLGEKQAAPRVSDLEALVTSTAGKIEIEAVGDAREDKVITKLLQGAVSAVF
                     NRHFTVQEFDDLLTRFHNGMNLEVSDSMPSMEYVHQASEVKALKNAVAKLGAQGNPAV
                     AASAVEFVLEGLHLNRRLNKDRSAGGVRYHR"
     gene            700..2727
                     /locus_tag="VF276_06920"
     CDS             700..2727
                     /locus_tag="VF276_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX6606632.1"
                     /translation="MPNYFVYRDWDGTQQIMPFDADAVMEAISDDLLADGDLRRALRR
                     LMQSGYQTRDNRRMMGLRDLMDRLRQRRQQMLKRHDMNSIMDDIAKQLDSIIQQERED
                     IQKKLDESHAQMQQQQAEGTAPPNAEQLQKMLDNMAAKHQQQLDNLPESPAGRIKELQ
                     DYDFMSPAAREQFQQLLAQLQQQVLNQTFKGLKGSMQGMTPEQRAELRNMMHDLNQML
                     RDRAEGREPRFKEFMEQYGHYFPKGIENLDQLMEHLAQQMQAMESLMQSMSAEQRAEL
                     ESLMEGLMQDEQLQAEMAELAANLQGMMPYEGERYPFSGDEPVTLSEAMRMMEQLQEM
                     EELEKQLDAARYGNNVDNIDAQKMSELVGAEEGQALQQLQELARMLEEEGYLENNGGK
                     MTLTPRGIRKIGQKALQDIFQYLKRDAFGKHATNLRGRGGERTDDSKEYEFGDPFYLD
                     LQATLMNAVERDGPGAPVRIDPTDFEVYRTELQTQSATVLMLDMSRSMLLRGCYLAAK
                     KVALALNHLIRTQFPRDTIYIVGFSAYARELKAEQLPQLDWSEYEYGTNLHHALLLGR
                     TLLARHKTPNKQIIVITDGEPTAHLEQGQSYFNYPPSWRTIQETLREVTRCTRDRIVI
                     NTFMLERTYDLTEFVNQMTAINKGRAFYATPEALGEYILVDYVNSKRKRIK"
     gene            2724..4409
                     /locus_tag="VF276_06925"
     CDS             2724..4409
                     /locus_tag="VF276_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606633.1"
                     /translation="MIRNPRNILLTCLLGAALIGCENGAPPTAPPIIPTAPPTAAATA
                     TASVPAPNTPTEAPAPATPTGPAATATSGLPPATPAAGVLTPPAAGSPTPSALHTTSG
                     LVFVSNGNLTFYDADHQETRALVEVVHDPPAPQRITAFAVAPTGDRLAYLLTEQRTAG
                     GACRAGTQLWLLDLNNINNRRQLAPSVLAEDCKERERTGVVQQGLAFAPDGARLAYLA
                     GRADGGPIDLWTVNSDTDAEAAPQRLAAPEAGLLLNPTWAPDGKSIAFLVANDLGTNG
                     SYDADLTILPVPADANTQPHTVAQGHALPGGHLALPPFDLTWLDPHTLAFQAWNPTRG
                     PDGGWVVDTAGTAAPQEIDRGQAGLGAWSPPGANGARWYAYRVAGLGLAVTSGATATG
                     ALLAPVPEAGPNGAVPPIWSADAGAVAFSTSDGRLLIADRAAPGAPIVAALPQVLHAA
                     WSATPGAPPLLAAVTSDGKTLTVFDRRGQPLHTETLSKVTGAPQEIVWEPAPAGADTQ
                     RIAVRYAGGPNDATPFVLFSFSSTGRGPGAQALAAVDDDSTLQWIMLGPLPEP"
     gene            4422..5585
                     /locus_tag="VF276_06930"
     CDS             4422..5585
                     /locus_tag="VF276_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HEX6606634.1"
                     /translation="MVEEPIKEQRLERRHVEERGYDPPAHPLFSESQVAFLSTARVAR
                     LATADAAGVPHVVPVCFAFDAPAAACYIALDQKPKRVAPTRLKRVRNILANPHAALLC
                     DVYREDWSRLSYCLVHAEAGLIDPGAAEHANALALLRAKYPQYRAMDLEAQPVIVLRA
                     TAVTSWAGGEAADDTAPDPALAAQRAALDFPALARGRRSVRVYAETPVPRPVVEQVLE
                     AGRWAPSPHGRLPWRFVVLTQPEPKRVLAEAMGDEWRRNLAMDGQSAEIVDIRLSKSY
                     QRLLRAPVLIIPCLYEADLDRYPDPARQAAERTMAVQSLGAAAQTMLLAAYSLGLDGG
                     WMCAPLFCPDVVRDALGIATNLQPQAILTLGYATSTPRRRLRLSLDELIVRYD"
     gene            5825..7963
                     /locus_tag="VF276_06935"
     CDS             5825..7963
                     /locus_tag="VF276_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003343242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HEX6606635.1"
                     /translation="MTPPVEAPAPETEVRTVICPHDCPDTCAMTATIENGRVVKMGAR
                     ADHPITKGFLCVKTQYYQERLYSPLRVLYPQRRVGPKGSGQFERISWDEAITAIAGRF
                     QGIIDRYGAEAILPYSYAGAMGKLSYASMDRRFFGYMGASLLDRTICSTTGAEAYKAV
                     MGTTMGTDPEGFPTARLIIAWGTNPVSTNVHLMPFIHEARSRGAQFVVVDPHRSKTAD
                     QADLHIRLNPGTDAALVLAMIHVMVRENLHDPAFLAAHTIGWEALEARAREWTPERAA
                     PITGLSAAQITEFARLYATTQPSVIRLSYGMTRHTNGGQNTRAVVMLPAVTGAWGQVG
                     GGALLSTSGTFGLDLTALERPDLLARHPHRPRTINMIQLAEALNDVTGPPVMGMLVYN
                     SNPANVAPNQRRVLAGLAREDLFTVVHEHLMTDTARYADILLPATTTFEQSDLFTAYG
                     HLYLQLGRPAIAPMGEAIPNTELFRRLAAAMGYDDPCFRDSDDDMMRQALSSEHPFLE
                     GITLERLEAETFVHLNTPTRPFVPFADGFYPTPSGKIELYSETLLRLGLDPVVDYVPE
                     AESPDGSPELAARYPIRLITPAAHHFLNSSFADMPTMRKKQRRPTIELNPADAAARGI
                     AGGTWVRAFNDRGEAFFVAEVQDSVGPGVACHLSRWWGRYSPAGWNANALTASRPADL
                     AGGGTFHTNLVQVEPAASVPPTLAQMLDEA"
     gene            7971..9059
                     /locus_tag="VF276_06940"
     CDS             7971..9059
                     /locus_tag="VF276_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006127279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4037 domain-containing protein"
                     /protein_id="HEX6606636.1"
                     /translation="MPDFIGGLELSRLFYIEVVRPLLAAAFPDLIYSAALIGAGSEIL
                     GYDTPRSTDHEWGPRLLLFLREADYATTGPCITEALRRQLPPTFHGYSTHFGPPDAEG
                     IQVATAYSDGPVEHKITVHTIRGFFMRQLGLDPSDALQPADWLTLSEQRLLEVTAGAV
                     YHDGLGELESIRARLAYYPRDLWLFLMASQWRRIAQQEAFVGRTGDVGDGLGSRLIAG
                     SIVRNLMRLCFLLERRYTPYSKWFGTAFARLACAPILGPILEQILTASTWQAREAALS
                     QAYGFVAGMHNALGVTPLLDPQVSSYYSRPYLVLHADRFVQALEDAVTDPAVRKLAAP
                     FGAVDQCTDSTDILSDPVVYRKLRLLYE"
BASE COUNT         1567 a         3301 c         2944 g         1326 t
ORIGIN      
        1 actgacggtg ccggagtata tgaaagagat cgtggccgaa ctgacccacc tggcgcgcaa
       61 gcacccggat atcagccagc gctcgggtgt atcggtgcgc gtgagcgtgt cgaactacga
      121 gaacctgttg agcaacgccg tcaagcgggc catccgcctg ggcgaaaagc aggccgcacc
      181 ccgcgtcagc gacctcgaag cgctggtcac atcgaccgcc ggcaagatcg agatcgaggc
      241 cgtgggcgac gcccgcgagg acaaggtgat cacaaagctg ctgcaaggcg ccgtcagcgc
      301 ggtgttcaat cgccacttca cggtgcagga gttcgacgac ctgctgaccc gcttccacaa
      361 cggcatgaac ctggaggtct ccgacagcat gccgtcgatg gagtacgtcc accaggccag
      421 cgaggtcaag gcgctgaaga acgccgtcgc caagctgggc gcgcagggca acccggccgt
      481 cgccgcatcg gctgtcgaat tcgtgttgga aggcttgcac ctgaaccgcc gcctgaacaa
      541 ggatcgctcg gcgggcggcg tgcgctatca tcgctaagcg ccccggcggc gggccgggcg
      601 ccgcgtgcgg ttcggcccct ggcgccggtg tacgcgcggc ttgacccaca cgccgcgcgg
      661 gcgcgccggg attggttcct cgcaagaaag aggcatccca tgcctaatta tttcgtctat
      721 cgcgattggg acggcacgca gcagatcatg ccgttcgacg ccgacgcggt catggaggcg
      781 atctccgacg acctgctggc cgacggcgat ctgcgccgcg ccctgcgccg cctcatgcag
      841 agcggctacc agacgcggga caaccgccgc atgatgggcc tgcgcgacct gatggaccgg
      901 ctgcgccagc gccgccagca gatgctcaag cgccatgata tgaacagcat catggacgac
      961 atcgccaagc aactggactc gatcatccag caggagcgcg aggacatcca gaagaagctg
     1021 gacgagagcc atgcccagat gcagcagcag caagccgagg gcaccgcgcc gcccaacgcc
     1081 gagcagttgc agaagatgct cgacaacatg gccgccaagc accagcagca actcgacaac
     1141 ctgccggaga gtcccgcggg ccgcatcaag gagctgcaag actacgactt catgagtccc
     1201 gcggcgcgcg agcagttcca gcagttgctg gcccagttgc aacagcaggt gctcaaccag
     1261 accttcaagg gcctcaaggg gtcgatgcag ggcatgacgc cggagcagcg cgccgaactg
     1321 cgtaacatga tgcacgacct gaaccagatg ttgcgcgacc gcgccgaagg gcgggagccg
     1381 cgcttcaaag agttcatgga gcagtacggc cactacttcc ccaagggcat cgagaacctc
     1441 gatcagctca tggagcacct ggcccagcag atgcaggcca tggaatcgct gatgcagagc
     1501 atgagcgccg agcagcgggc cgagttggag agcctgatgg aagggctgat gcaggacgag
     1561 cagttgcagg ccgagatggc cgaactggcc gccaacctgc aaggcatgat gccctacgag
     1621 ggcgagcgct atcccttctc cggcgacgaa cccgtgaccc tcagcgaggc gatgcggatg
     1681 atggagcagc ttcaagagat ggaagagctt gagaagcagc ttgacgccgc ccgctacggc
     1741 aacaacgtcg acaacatcga cgcccagaag atgagcgaac tggtcggcgc cgaagagggg
     1801 caggccctcc agcagttgca ggaactggcg cgcatgctgg aggaagaggg gtacctcgaa
     1861 aacaacggcg gcaagatgac cctgacgccg cgcggcatcc gcaagatcgg ccagaaggcg
     1921 ttgcaagaca tcttccagta cctcaagcgc gacgctttcg gcaagcacgc gacgaacctg
     1981 cgcgggcgcg gcggcgagcg caccgacgac agcaaggagt acgagttcgg cgatcccttc
     2041 tacctggact tgcaggccac gctgatgaac gccgtggagc gcgacggccc cggcgcgccc
     2101 gtccgcatcg acccaaccga cttcgaggtc taccgcaccg agttgcagac gcagagcgcc
     2161 accgtgctca tgctcgacat gagtcgctcg atgttgctgc gcggctgtta cctggcggcc
     2221 aagaaggtgg ccctggcgct caaccacctg atccgcacgc agttcccgcg cgacacgatc
     2281 tatatcgtcg gcttctcggc ctatgctcgc gaactcaagg ccgagcagtt gccgcaactc
     2341 gactggagcg agtacgagta cggcaccaac ctgcaccacg cgctgctgct aggccgcacc
     2401 ctgctggccc gccacaagac gcccaacaag cagatcatcg tcatcaccga cggcgagccg
     2461 acggcgcacc tggagcaagg ccagtcgtat ttcaactacc cgccaagctg gcgcacgatc
     2521 caggagacgc tgcgcgaggt cacgcgctgc acccgcgacc gtatcgtgat caacaccttc
     2581 atgctggagc ggacgtacga cctgactgag ttcgtcaacc agatgacggc gatcaacaag
     2641 ggccgggcct tctacgccac gcccgaggcg ctgggcgagt atatcctggt cgactacgtg
     2701 aacagcaagc gcaagcgtat caagtgatcc gcaacccgcg aaacatcttg ctcacctgcc
     2761 tgctgggcgc cgctctcatc ggttgcgaga acggcgcccc gccgacagcg ccgccgatca
     2821 ttccgaccgc accgcccacg gcagccgcta ccgcgacggc cagcgttccc gcccccaaca
     2881 cacccaccga ggccccggca cccgccacgc cgaccggccc cgccgccacc gcgacgagtg
     2941 gcctgccgcc ggccacgccc gccgcgggag tgctgacccc accggctgct ggtagcccga
     3001 ctccttccgc gctgcacacc accagcggcc tggttttcgt gagcaacggc aacctcacct
     3061 tctacgacgc cgaccaccag gagacgcggg cgctggtcga ggtggtccac gacccgcccg
     3121 ccccgcagcg catcacggcc ttcgccgtcg cgcccaccgg cgacaggctg gcctacctgc
     3181 taaccgagca gcgcaccgcc ggcggcgcct gccgtgccgg cacgcaactc tggctgctgg
     3241 acctgaacaa catcaacaac cgtcgccaac tggcgccatc ggtgctggct gaggactgta
     3301 aggaacggga gcgcaccggc gtcgtccagc aggggctggc ctttgccccc gacggcgcgc
     3361 gcctggccta cctggccggg cgggccgacg gcgggccgat tgacctctgg accgtgaaca
     3421 gcgacaccga cgccgaggcc gcgccccagc ggctggccgc gcctgaagcc ggtctgctgc
     3481 tgaaccccac ctgggcaccc gacggcaagt ctatcgcctt cctggtggcg aacgacctgg
     3541 gcaccaacgg cagctacgat gccgacctga ccatcctgcc cgtgcccgcg gacgccaaca
     3601 cccagcccca cacggtcgcc cagggccacg cgttgcccgg cggccacctg gcgctgccgc
     3661 ccttcgacct gacctggctc gacccgcaca cgctggcctt ccaggcgtgg aacccgacgc
     3721 gcggcccgga cggcggctgg gtcgtggata ccgcgggcac cgccgcgccc caggagatcg
     3781 accgcgggca ggccgggctg ggcgcctgga gtccgcccgg cgcgaacggc gcgcgctggt
     3841 atgcctaccg ggtggcgggc ctgggtctgg ccgtgacctc cggcgccacc gcgaccggcg
     3901 cattgctggc cccggtgccc gaagccggcc ccaacggcgc cgtcccgccg atctggtccg
     3961 ccgatgccgg cgcggtggcc ttcagcacca gcgacggccg cctgctcatc gccgaccggg
     4021 ccgcgccggg cgccccgatc gtcgccgcgc tgccccaggt gctgcacgct gcctggagcg
     4081 ccacgccggg cgcgccccca ttgctggccg ccgtcacctc cgacggcaag acgctgaccg
     4141 tgttcgaccg gcgcggacag cccttgcaca ccgagacact gagcaaagtc accggtgcgc
     4201 cgcaggaaat cgtctgggag ccggcgcctg cgggggccga cacccaacga atcgccgtcc
     4261 gctacgccgg tggcccgaac gatgccacgc cgttcgtgct gttcagcttc agcagcacgg
     4321 ggcgcggccc cggcgcccag gccctcgccg ccgtggacga cgactcgacg ctgcaatgga
     4381 tcatgctcgg cccgctgccg gagccgtagg tagagatgcg tatggtagag gaacctatca
     4441 aggagcaacg cctcgagcgc cggcacgtcg aggagcgcgg ctatgacccg ccggcgcatc
     4501 cgctgttcag cgaaagccag gtggccttcc tgagcacggc gcgggtggcg cggttggcga
     4561 ccgccgatgc cgcgggcgtg ccccatgtag tgccggtctg ctttgccttc gacgcgcccg
     4621 ccgcggcctg ctatatcgcg ctggaccaga agccgaagcg ggtagcgccc acgcggctca
     4681 agcgcgtgcg caacatcctg gcgaaccccc acgcggcgct gctgtgcgac gtgtaccgcg
     4741 aggattggag ccgcctcagc tattgcctgg tccacgccga ggccgggctg atcgatcccg
     4801 gcgccgccga gcacgcgaac gccctggctc tgctgcgggc caagtatccg cagtaccggg
     4861 cgatggacct ggaggcccag ccggtgatcg tgctgcgcgc gaccgccgtc acctcgtggg
     4921 cggggggcga agcggcggac gatacggcgc cggacccggc gctggcggcg cagcgggccg
     4981 cgctcgattt cccggccctg gcccgcgggc ggcgctcggt gcgcgtctat gccgagacgc
     5041 cggtgccccg gcccgtggtc gagcaggtgc tggaggccgg gcgctgggcg ccctcgccgc
     5101 acgggcgcct gccctggcgc ttcgtcgtgc tgacccagcc ggaacccaag cgcgtgctgg
     5161 ccgaggccat gggcgacgag tggcggcgta acctggcgat ggacggccag tccgccgaga
     5221 tcgtggacat ccgcctcagc aagtcgtacc agcgcctgct gcgcgccccg gtgctgatca
     5281 tcccctgcct ctacgaagcc gacctggacc gctaccccga cccggcgcgc caggccgccg
     5341 agcgcaccat ggccgtgcag agcctgggcg ccgccgcgca aacgatgttg ctggcggcct
     5401 acagcctggg cctggacggc ggctggatgt gcgcgcccct cttctgcccg gacgtggtcc
     5461 gtgacgccct cggcatcgcc accaacctgc aaccgcaggc catcctcacg ctcggttacg
     5521 cgacgagcac cccgcgccgc cgcctgcgcc tgtctctcga tgagttgatt gtccgatatg
     5581 attagcccat tacctgggca gggcgccgtg gtataatgat agacagcata gcacccagcc
     5641 gagataatct tgcctgtagg ggccggtttg aaaccggccc gtcgttcggt cgcgctgtag
     5701 gggccggttg ctcggctcga tcttgttgta ggggccggtt tcaaaccggc ccggtgcccg
     5761 atcgcgttgt aggggccggt ttgaaaccgg ccttgcctgc gttagcagca caggagtatt
     5821 gcccatgacc ccgcccgtcg aagcgcccgc gccggaaacc gaggtgcgca ccgtcatctg
     5881 cccgcacgac tgccccgata cctgcgccat gaccgccacc atcgaaaacg gccgggtggt
     5941 caagatgggt gcccgcgccg atcaccccat caccaaaggc ttcctctgcg tcaaaacgca
     6001 gtactaccag gaacgcctct attcgccgct gcgcgtgctc tatccgcagc gccgggtggg
     6061 gccgaagggc agcgggcagt tcgagcgcat ctcctgggac gaggccatca ccgccatcgc
     6121 cgggcgcttc cagggcatca tcgaccgcta cggcgcggag gccatcctgc cctacagcta
     6181 cgcgggcgcc atgggcaagc tgtcctacgc cagcatggac cgccggttct tcggctatat
     6241 gggcgcctcg ctgctcgacc gcactatctg ctcgaccacc ggcgccgagg cgtacaaggc
     6301 cgtcatgggc acgaccatgg gcaccgaccc cgaaggcttc cccacagccc ggctgatcat
     6361 cgcctggggc accaacccgg tctcgaccaa cgtccacctg atgccgttca tccacgaggc
     6421 gcgcagccgc ggcgcgcagt tcgtggtggt ggacccgcac cgcagcaaga ccgccgacca
     6481 ggccgacctg cacatccgcc tcaaccccgg cacggacgcc gcgctggtgc tggcgatgat
     6541 ccacgtcatg gtgcgcgaga acctgcacga tcccgccttc ctggccgcgc acaccatcgg
     6601 ctgggaggcg ctggaggcgc gcgcccgcga gtggacgccc gaacgcgccg cgccgatcac
     6661 cggcctgagc gccgcgcaga tcaccgagtt cgcccggctc tacgcgacca cgcagcccag
     6721 cgtcatccgc ctgagctacg gcatgacgcg gcacaccaac ggcgggcaga acacccgcgc
     6781 cgtggtgatg ctgcccgccg tgaccggcgc ctggggtcag gtgggtggcg gcgcgctgct
     6841 cagcaccagc ggcacctttg ggttggacct gacggccctg gagcggcccg acctgctggc
     6901 ccggcacccg caccgcccgc gcaccatcaa catgatccaa ctggccgagg cgctcaacga
     6961 cgtgaccggc ccgccggtga tgggcatgct ggtctacaac tcgaacccgg ccaacgtcgc
     7021 gccgaaccag cggcgggtgt tggcggggct ggcccgcgaa gacctgttca ccgtcgtcca
     7081 cgagcatttg atgaccgaca cggcgcgcta cgccgacatc ctgctgcccg ccacgaccac
     7141 cttcgagcag agcgacctgt tcaccgccta cggccatctc tatctgcaac tgggccgccc
     7201 ggccatcgcg ccgatgggcg aggcgattcc caacaccgaa ctgttccgcc gcctggccgc
     7261 cgccatgggc tacgacgatc cctgcttccg cgacagcgac gacgacatga tgcgccaggc
     7321 cctcagcagc gagcacccgt tcctggaggg catcacgctg gagcgcctgg aagccgagac
     7381 gttcgtccac ctcaacacgc ccacgcgtcc cttcgtgccc ttcgccgacg gcttctatcc
     7441 cacgccgtcg ggcaagatcg aactctactc ggaaacgctg ctgcgcctgg gcctcgaccc
     7501 ggtggtcgat tatgtgcccg aagccgaaag cccggacggc tcgcccgaac tggccgcgcg
     7561 ctaccccatc cggctgatca ccccggcggc gcaccatttc ctcaactcgt ccttcgccga
     7621 catgccgacc atgcgcaaaa agcagcgccg cccgaccatc gaactcaatc ccgccgacgc
     7681 ggcggcgcgc ggcatcgccg gcggcacctg ggtgcgcgcg ttcaacgacc ggggcgaggc
     7741 gttcttcgtg gccgaggtgc aggacagcgt ggggccgggg gtggcctgcc acctgagccg
     7801 gtggtggggc cgctacagcc ccgccgggtg gaacgccaac gcgctcacgg ccagccgccc
     7861 cgccgaccta gccggcggcg gcaccttcca caccaacctg gtgcaggtgg aacccgccgc
     7921 atccgtgccg cccaccctgg cccagatgct ggacgaagcg tagcacgtcc gtgcccgatt
     7981 ttatcggcgg tctggagcta agccggctgt tctacataga ggtcgtgcgc ccgctcctgg
     8041 ccgccgcgtt ccccgatcta atctactcgg cggccctcat cggcgccggc tctgagattc
     8101 tgggctatga tacgccgcgc tccaccgacc atgagtgggg gccgcggctc ctgctgttct
     8161 tgcgggaagc cgattacgcc actaccggcc cgtgtatcac ggaggcgttg cgccggcaac
     8221 tgccacccac cttccacggt tactccacgc acttcgggcc gccggacgcc gaggggatcc
     8281 aggtcgcgac agcgtatagc gatgggccgg tggaacacaa aatcacggtt cataccatcc
     8341 gcgggttttt tatgcgccaa ctgggcctcg acccgtcgga cgctctgcaa ccggccgatt
     8401 ggctcacgct gtctgagcaa cggctattgg aggtgacggc cggtgccgtc taccacgatg
     8461 gcctggggga gttggaatcg atccgcgccc ggctggccta ttatccgcgc gacctctggc
     8521 tgttcctcat ggcgagccag tggcggcgca ttgctcaaca ggaagccttc gtgggccgga
     8581 ccggcgacgt gggcgacggc ctgggttccc ggctcatcgc cggcagcatc gtccgcaatc
     8641 tgatgcgcct ctgcttcctg ctggagcgcc gctacacgcc gtacagcaag tggttcggta
     8701 ccgccttcgc ccggcttgcc tgcgcgccta tcctgggccc gatcctggag cagattctaa
     8761 ctgcatccac ctggcaggcg cgcgaggccg ccttatcgca ggcttatgga ttcgtggccg
     8821 ggatgcataa cgcactgggc gtcaccccgc tgctggaccc gcaggtgtcg tcgtattata
     8881 gccggcccta cctcgtcctc cacgcggatc gctttgtcca ggccctggaa gatgccgtca
     8941 ccgacccggc ggtgcgcaaa ctggcagctc ccttcggcgc ggtagaccag tgcaccgaca
     9001 gcacggacat cctgtccgat ccggtggtat accggaagct acggctgctt tatgagtgag
     9061 ccgtggaatg gggtcgcccc gtcgccgagg ccggtggagt ctttgtagat cgcgtatttg
     9121 caatgggccg cgcccgtc
//
LOCUS       DASUYC010000421         2187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_744510,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000421 DASUYC010000000
VERSION     DASUYC010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744510"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VF276_06945"
     CDS             complement(<1..458)
                     /locus_tag="VF276_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606637.1"
                     /translation="MKSGIAQPGRDVTPLMRLFMVFAGLIVVVGIVDVLLLFQPFALR
                     PNIQHLRTYPNARNMEEVHTPGTWAGISPKGRSLTTETDQISFQTTDSPGAVFTFYIS
                     VLNKDGWADSTPLVGPKTERDFQLLTGDPETAFDLRILTNSEEGAATRVQ"
     gene            complement(546..>2187)
                     /locus_tag="VF276_06950"
     CDS             complement(546..>2187)
                     /locus_tag="VF276_06950"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX6606638.1"
                     /translation="FQAVSQLNGRDSVGVVTFDEAADWAVPLAKLGDPAALRDKIGSI
                     GPGGGTYIYSGLAQAVDALEKSNARAKHIVLLTDGQSQGGDYPGLLARMSTAKITLST
                     VGLGSDVDASLLNSLAAQGGGRFYNTLDAGSLPAIFAHESHLASRSYLIEHHFTPKRT
                     APSPILQNIADTPPLLGYVGTTVRPEAVLALVSDSGDPVLAHWQYGLGRVAAWTSDAK
                     GRWATDWIGWKEFPTFWGAAERWAMGTDNGGGLQARVELQDNQARVSLDAVSGSGEPI
                     NQLHPLAAVVPPAQAGAPVSQSLTLRQTAPGHYEGTFPAGAEGAYLVRVQAGTPGAAG
                     SSAQTVGLIVPYSPEYRALPGNNGLLARLTALTGGRTLALDTPSEQAVFNHDLPEVSR
                     AVDLWPWLLLLALLLLPLDIGARRVLVGWRDIPRFWAEVRGRLRPAVAPVVPSDASAS
                     AELGPLFEARRAANRTTRPAAGEIAARLDREQRRTAQADTAPPPGPQPLQGPQTGVQL
                     TGRTTSAPPPPAPPAAPSGPAPGNLAGEVLRRRRQREE"
BASE COUNT          349 a          716 c          778 g          344 t
ORIGIN      
        1 atctgcacgc gagtcgccgc tccctcttcg gagttagtca aaatgcgaag atcaaatgcc
       61 gtctctggat ctcctgtcag cagttgaaag tcccgctcag tctttggtcc aacaagcggg
      121 gtcgagtcag cccatccgtc cttgtttagc accgagatat agaaggtaaa aaccgcgcca
      181 ggggaatccg tggtctggaa agaaatctga tcagtctctg tggttaagct gcggcccttg
      241 gggctgattc cagcccaggt gccaggcgta tgcacttcct ccatattccg tgcgttggga
      301 tacgtgcgaa gatgctggat attagggcgc aacgcaaagg gttggaagag gagcaagaca
      361 tcaacaatgc ctacaacgac aataaggcca gcaaagacca taaacaatcg catcaacgga
      421 gttacatccc ttccaggctg agcgatcccg gatttcaatt cgatctcctt cttcgacaag
      481 caccaattaa agcgcgctct tttacagcca attgaaatgg agttggtctg cgctcagtgc
      541 aaaccctact cctcgcgctg gcggcggcgg cgcaggactt cgccagccag gttgccgggg
      601 gcaggaccgg agggcgcggc gggcggcgcg ggaggcgggg gcgcagatgt ggtgcgcccg
      661 gtgagctgga cgccggtttg tggcccctgg agcggctgcg ggccgggcgg cggcgccgta
      721 tcggcctggg cggtgcgccg ctgctcacgg tcgaggcggg cggcgatttc gcccgccgcc
      781 gggcgggtgg tgcggttggc ggcgcggcgg gcctcgaaca gcggccccag ctcggcgctg
      841 gccgaggcgt cgctgggcac gacgggcgcg acggcggggc gcagccgccc gcgcacctcg
      901 gcccagaagc gcgggatgtc acgccagccc accagcaccc ggcgcgcgcc gatatcgagc
      961 ggcagcagca gcagcgccag caacagcagc cacggccaga gatcgacggc ccgcgacacc
     1021 tccggcaggt cgtggttgaa caccgcctgc tcgctgggcg tatcgagggc cagcgtgcgc
     1081 ccgccggtca gggccgtgag gcgggccagc aggccgttat tgcccggcaa ggcgcgatat
     1141 tcgggtgagt agggcacgat gagcccgacc gtctgcgcgc tgctgcccgc ggcccccggc
     1201 gtgccggcct gcacgcgcac caggtaggcg ccctccgcgc ccgccgggaa ggtgccttcg
     1261 tagtgacccg gcgcggtctg gcgcagggtc agcgactggc tcaccggcgc cccggcctgc
     1321 gcgggcggca ccacggcggc cagcgggtgc aactggttga tcggctcgcc gctgccgctg
     1381 acggcgtcga ggctgacgcg ggcctggttg tcttgcagtt cgactcgcgc ctgcaagccg
     1441 ccgccgttgt cggtgcccat ggcccagcgt tcggcggcgc cccagaaggt ggggaattcc
     1501 ttccagccga tccagtcggt ggcccagcgg cccttcgcgt ccgaggtcca ggcggccacc
     1561 cgaccgaggc cgtactgcca gtgggccagc acggggtcgc cgctgtcgga gacgagggcc
     1621 agcaccgcct ccgggcgcac ggtcgtgccg acgtagccca gcaggggcgg cgtgtcggcg
     1681 atgttttgca ggatcgggct gggcgcggtg cgcttgggcg tgaaatggtg ctcgatcagg
     1741 taggagcgcg aggccaggtg gctctcgtgg gcgaagatgg cgggcaggct cccggcatcg
     1801 agcgtgttat agaagcgccc gccgccctgc gccgcgaggc tgttcagcag gctcgcgtcc
     1861 acgtccgagc cgaggccgac cgtggacagc gtgatcttgg cggtgctcat gcgcgccagc
     1921 aggccggggt aatcgccgcc ctgcgactgg ccgtcggtca gcaggacgat atgcttcgca
     1981 cgggcgttgc ttttctccag cgcgtccaca gcctgggcca gacccgaata gatgtaagtg
     2041 ccgccgcccg gcccgatgct gccgatcttg tcgcgtaacg ccgccgggtc gcccagcttc
     2101 gccagcggca cggcccagtc cgcggcctcg tcaaaggtca cgacgccgac cgagtcgcgg
     2161 ccgttcaact ggctgacggc ctggaac
//
LOCUS       DASUYC010000422         2355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_518963,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000422 DASUYC010000000
VERSION     DASUYC010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518963"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="VF276_06955"
     CDS             complement(<1..670)
                     /locus_tag="VF276_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606639.1"
                     /translation="MDQQTTIVGRPTYMAAFAGAQDAGAIVDKLTSIGYPREDISVLL
                     RPAGSDSAIDLLSGENAAGQDTNAMAQQKHKEGEQPTTLVLLHPNAAQVEAVRAALTG
                     MGAKEVEYEPETRYTGEDSEADFVKSTIGNVDADSGLGAFGSAHPSDANDTEGGTEPG
                     HFNPPPNDATSDAPPQASGSGNIPVPTPEGTHAEMAAGSASAGADSGSQTGSAARTNT
                     EAPPP"
     gene            complement(855..1562)
                     /locus_tag="VF276_06960"
     CDS             complement(855..1562)
                     /locus_tag="VF276_06960"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX6606640.1"
                     /translation="MKKLLFTLMAAALLLTLSTTAAWAQTGTGTVTGKAVRWNGQPIA
                     GATIRALTGPLETDKEVDRTTTAADGSYSLTVPVGQPYWVHIDTFGTWWGYSYQTPFT
                     LRPGETISQVFFALGPRDVKEIALPAPVSNVGPATGENPPPQVSEPPAQAPKPIEQAP
                     APQPNAPVSNVKPEVGTNPAPAPAAAPKPAPRPAPQTLPRTGEGEGNDALWLGLTGAL
                     VLLLAGLTIRQAVTTRR"
     gene            complement(1894..>2355)
                     /locus_tag="VF276_06965"
     CDS             complement(1894..>2355)
                     /locus_tag="VF276_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606641.1"
                     /translation="VALLLAAAAPDLIRAVVAVDVSGAFPDALLAVLPEIGNWADDPQ
                     TPNLARRTTAIREYGLEGTQAIWAGWKQAVRRIIAAGGNVSLEQARAIRCPVLIVNHD
                     DDPLNTPAVSRRLRDAIPNAELDLLAGIDPRTFDAHFYWYTPHVQRWLADH"
BASE COUNT          323 a          733 c          873 g          426 t
ORIGIN      
        1 ccggtggcgg ggcctcagta ttagtacgcg cggcgctgcc tgtttggctt cccgagtccg
       61 cgccggcgga cgccgagccg gcagccatct cggcgtgcgt gccttcaggc gtgggcacgg
      121 gaatattgcc gctgcccgat gcctggggcg gcgcgtcgga cgtggcgtcg ttgggcggcg
      181 ggttgaaatg gccgggttcg gtgccgccct cggtatcgtt ggcgtctgag gggtgggccg
      241 agccaaaggc gcctagcccg ctgtcggcgt cgacgttgcc aatggtcgat ttcacgaaat
      301 cggcctccga gtcctcgccg gtatagcggg tttcgggctc atactccact tctttcgcgc
      361 ccatgccggt cagcgcggcg cgcaccgctt ccacctgcgc ggcgttgggg tgtagcaaga
      421 ccagcgtggt cggttgttcg ccctctttgt gcttctgttg cgccatggcg ttggtatcct
      481 gcccggcggc gttttccccg ctgagcaggt cgattgccga atcgctgccg gcgggccgca
      541 gcaagaccga gatgtcctcc cgcgggtagc cgatactggt cagtttgtcc acgatcgcgc
      601 ccgcatcctg ggcgccggca aaggccgcca tatacgtggg gcgaccgacg atggtggtct
      661 gttggtccat ggccgggatc ctccttggag cgcggattcg cgcggctggg tgcagtgtat
      721 cacgggggtg acagagcgtc aacccgttgg cgcctcctgt gcatgttctg tgccttgcgc
      781 aacaaggaag ggaccccgcg gggtcccttc cggcctgatg gttggcgcgg tccgccggga
      841 ccgcgcgggg caggttagcg ccgggtggtc acggcctggc ggatagtcag cccggcgagc
      901 agcagcacca gggcgccggt caagcccagc cagagcgcgt cgttgccttc gccctcgccg
      961 gtgcgcggca aggtctgcgg cgcagggcgc ggggccggtt tcggcgcggc ggcgggcgcc
     1021 ggggccgggt tcgtgcccac ttcgggcttc acgttggaga ccggggcgtt gggctgcggt
     1081 gcgggcgcct gctcgatcgg cttgggcgcc tgggcgggcg gttcgctcac ctgcggcggc
     1141 ggattctcgc ccgtggccgg gccgacgttg ctcaccgggg caggcaaggc gatctctttc
     1201 acgtcgcgcg gccccagggc gaagaagacc tgcgagatgg tctcaccggg ccgcaacgtg
     1261 aacggcgtct gatagctgta gccccaccag gtgccgaagg tgtcgatgtg cacccagtac
     1321 ggctggccca ccgggacagt cagcgaatac gagccgtcgg cggcggtggt cgtgcggtcc
     1381 acttccttat ccgtctccag cgggccggtc agcgcgcgga tggtggcccc ggcaatgggc
     1441 tggccgttcc agcgcaccgc cttgccggtg acggtgccgg tgccggtttg cgcccaggct
     1501 gcggtcgtgc tcagggtcag caagagagcg gcagccatca gggtaaaaag gagcttcttc
     1561 atgggcttac ctctttcctc gaagggtata aagcgtatgg aactgtatgg gacctgaacg
     1621 tgagtgcggg atgtaatgat gttgtcccgg aaccacggtc gctatgcggc gcctccaccg
     1681 cggttctatc ccctgtaaaa gcatagggtg tgccagactg agccgaaacg ggggtctggt
     1741 tgtgcccaat tacgccgatt atccgtagta cttttggggg atagcggcta gtaccggctg
     1801 ccggatcagc acgccgatcc ggcgcatccc tcaatagata cgacgtacga gccagggcat
     1861 cggttacgat cggggagtcg ggggcgccgc gggtcagtga tcggccagcc agcgctgcac
     1921 gtgcggggta taccagtaga agtgggcgtc gaaagtccgc gggtcgatgc cggcgagcag
     1981 gtcgagttcg gcgttgggga tggcgtcgcg caggcgccgg ctcactgcgg gcgtgttcag
     2041 cgggtcgtcg tcgtgattga cgatcaggac ggggcagcga atggcgcgcg cttgctccag
     2101 gctgacattg cccccggcgg cgatgatccg gcgcaccgcc tgtttccagc ccgcccagat
     2161 ggcctgcgtg ccctccagcc cgtactcgcg gatggccgtg gtgcggcggg ccagattggg
     2221 agtctggggg tcgtcggccc agttgccgat ctccggcagc accgccagca gcgcgtccgg
     2281 gaaggcgccg gacacatcga ctgcgaccac cgcgcggatc aggtccggcg cggcggcggc
     2341 cagcagcagc gcgac
//
LOCUS       DASUYC010000423         3315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1641968, whole genome shotgun sequence.
ACCESSION   DASUYC010000423 DASUYC010000000
VERSION     DASUYC010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1641968"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /locus_tag="VF276_06970"
     CDS             complement(<1..1116)
                     /locus_tag="VF276_06970"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX6606642.1"
                     /translation="MAQASSPAQARMVTTTQQEPPRGGAPLTRLLALAFPYWPRLTAG
                     GLMLVLNSFITLAIPLTVQQLVDGITGPRRMDSLGQSILLLVAVSVISATLNFGQSYS
                     LAYVGERLVADLRRRVFTHLQSLSLTFYENQRVGELTSRLANDVSVVQAGLTTNLFGA
                     VQQMITLIGGLFFIAIFDWRLLLLIVVLVPPLILIGRTLGKRLETTSKQSQEALGEAT
                     TVLEETLSAPRVVKAFNREDYESERYGQAIEKTFEIAVRRIRLGAAFGPIMTMMGFGV
                     MIAILWFGGSEVVAGQLTTGQLVSLLLFVVMVSGPLGGLAATYAQLRAAGGAAARVFE
                     LLDTRVEVVEAPDAVPLPEVAGAISFEHATFHYAGGAD"
     gene            1529..2701
                     /locus_tag="VF276_06975"
     CDS             1529..2701
                     /locus_tag="VF276_06975"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016948893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEX6606643.1"
                     /translation="MKIAVPKEILPGERRVALTPDAAGRLVKAGHEVLVEAGAGTAAY
                     FPDAAYTAAGAQLVADTAALFGAGDIVLKVQKPMLNEALGRHEVDLLKPGAVLISFLA
                     SLTSPDLVRRLAEHNITSFGMEAIPRISRAQTMDALSSQATVAGYKAVLIAADTLGKF
                     FPLLMTAAGTVAPAKVFVLGAGVAGLQAIATARRLGAVVEAFDVRPVVKEQVESLGAK
                     FVMLEQGAIEAAGAGGYAKELSEEQHRQEVALLARHAKDSDVIITTAAIPGRRAPVLI
                     TADMVATMRPGSVIVDIAAETGGNCELTQAGETVVQNGVLIMGPLNMPSMIPVHASQM
                     YSKNVTNLLQLLMPKTELQLNFDDEIVSGTCITHGGQVVHAATRKALEAASQGVAA"
     gene            2698..3024
                     /locus_tag="VF276_06980"
     CDS             2698..3024
                     /locus_tag="VF276_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEX6606644.1"
                     /translation="MSGLSIEALLLIITIFILAIFVGIEVISKVPTVLHTPLMSGTNA
                     IHGIVLMAAMLVAGIAVAGKGWGLMLIGFLAVVLGTINVVGGFVVTDRMLEMFKAKQP
                     PKETKK"
     gene            3109..>3315
                     /locus_tag="VF276_06985"
     CDS             3109..>3315
                     /locus_tag="VF276_06985"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEX6606645.1"
                     /translation="MIPSDSPILQIIYILAAVAIIIGLKRLSSPATARTGNLIAAVGV
                     GIALLVTLFTSDIHDYLWIFIGVPI"
BASE COUNT          595 a         1075 c         1085 g          560 t
ORIGIN      
        1 atcggcgccc ccggcatagt gaaaagtggc gtgctcgaag ctgatcgccc cggccacctc
       61 cggcagcggc acggcgtccg gcgcctcgac tacctccacg cgggtgtcca gcaactcgaa
      121 gacgcgcgcc gccgcgcccc ccgccgcccg caactgggca tacgtggcgg ccaggccgcc
      181 cagcggcccc gagaccatga ccacgaacag cagcagcgag accagttgcc cggtggtcag
      241 ttgcccggcc accacttcgc tgccgccgaa ccacaggatg gcgatcatca ccccgaagcc
      301 catcatggtc atgatcggcc cgaaggccgc gcccaggcga atgcggcgca cggcgatctc
      361 gaaggttttt tcgatggcct gcccgtagcg ctcggactcg tagtcctcgc ggttaaaggc
      421 tttgaccacc cgcggcgccg acaacgtctc ttcaagcacg gtcgtcgctt cgcccagcgc
      481 ttcctgcgac tgcttggacg tggtctccag ccgcttgccg agcgtccgcc cgatcaggat
      541 cagcgggggc accagcacga cgatcagcag gagcagccgc cagtcgaaga tggcgatgaa
      601 gaagaggccg ccgatcagcg taatcatctg ctggacggcg ccgaacaggt tggtcgtcag
      661 cccggcctgg accacgctca cgtcgttcgc caggcgcgag gtgagttcgc ccacgcgctg
      721 gttctcatag aaggtgagcg agaggctttg caggtgggtg aacacgcgcc ggcgcaggtc
      781 ggccaccagg cgctcgccca cataggccag cgaatacgac tggccgaagt tcagcgtggc
      841 gctgatcacc gagacggcga ccagcagcag aatgctctgc cccagactat ccatgcgccg
      901 cggcccggtg atgccgtcga ccagttgctg caccgtgagc gggatcgcca gggtgatgaa
      961 gctgttgagc accagcatga ggccgcccgc cgtcagacgg ggccaatagg ggaaggccag
     1021 ggccagcagc cgcgtgagcg gcgcaccgcc gcgggggggc tcttgctgcg tggtcgtgac
     1081 catccgggcc tgggccgggc tgcttgcctg cgccaaatcg cgatccttcc caccggggcc
     1141 ggcgccccca cactacgcgg gccggacacg tgcccggccc accgctgctg tctgccctga
     1201 ctacgcacat agaacgaaaa ggttccagga agcttacggt tttcgccgcc ccgtggttct
     1261 cttgacagtc gaggggacgt tgtgctagag ttggctgaga ttccggacgc gtcccccgga
     1321 aacgcacggt gctgcgggat cgcggctgca acatgctgcc cgcggcgtgg cgggattttc
     1381 accatttgtc tacggacgtt cacgttgccg gcaacctgca tcgcctatac tagatgctgg
     1441 atcaaaaagg gccaatcaca gggttctcct ggatggacga cgctggaggt agcgacatgc
     1501 ttcatcactc caaaggtagg aagggtttat gaaaatcgca gttccgaaag agattctgcc
     1561 cggagagcgc cgcgtggcgc tcactcccga tgccgccggc cgcctggtga aagccgggca
     1621 cgaagtcctg gtggaggcgg gcgcgggcac cgccgcctat ttccccgatg cggcctatac
     1681 cgcggcgggc gcgcaactcg tggccgatac ggcggcgctg ttcggcgccg gcgatattgt
     1741 gctcaaagtg cagaagccga tgctcaacga ggcgctgggc cggcacgaag tcgatctgct
     1801 caagccgggc gccgtcctga tcagcttcct ggcctcgctg accagccctg acctggtgcg
     1861 ccggctggcc gagcacaata ttaccagctt cggcatggag gcgatcccgc gcatctcgcg
     1921 cgcgcagacg atggacgccc tgtcctcgca ggccaccgtc gccggctaca aggcagtgct
     1981 catcgccgcc gacacgctgg gcaagttctt cccgctgctg atgacggcgg cgggcaccgt
     2041 ggcaccggcc aaggtttttg tgctgggcgc cggggtggcg ggcttgcagg ccatcgccac
     2101 ggcgcgccgc ctgggcgcgg tggtcgaggc cttcgacgtg cgcccggtgg tgaaggaaca
     2161 ggtcgagagc ctgggcgcca agttcgtcat gctcgaacag ggtgccattg aggccgccgg
     2221 cgcgggcggc tacgccaagg aattgtccga agagcagcat cgccaggaag tggcgctgct
     2281 ggcccgccat gctaaggact ccgatgtgat tatcaccacg gcggccattc cggggcggcg
     2341 cgcgccggtg ctgatcaccg ccgacatggt cgccaccatg cggcccggct cggtgatcgt
     2401 cgatattgcc gcggagaccg gcggcaactg cgagctgacg caagcgggcg agaccgtcgt
     2461 gcagaacggc gtcctcatca tggggccgct gaacatgccg agcatgatcc cggtccacgc
     2521 cagccagatg tattcgaaga acgtgaccaa cctgctgcaa ctgcttatgc ccaagaccga
     2581 gttgcagttg aattttgacg acgaaatcgt cagcggcacc tgcatcacgc acggcggcca
     2641 ggtcgttcat gccgccacgc gcaaagcgct ggaagccgcc tcgcaaggag ttgccgcatg
     2701 agtggcctca gtatcgaagc cctgttgctc attataacga ttttcatttt ggcaatcttc
     2761 gtcggcatcg aagtgatcag taaagtcccg accgtgctgc acacgccgct gatgtcgggc
     2821 acgaacgcga tccacggcat cgtgctgatg gcggccatgc tggtcgcggg cattgccgtc
     2881 gccggcaagg gctggggcct gatgctgatc ggcttcctgg ccgtggtgct gggcaccatc
     2941 aacgtggtcg gcggcttcgt cgtaaccgac cggatgctcg aaatgttcaa ggcgaaacaa
     3001 ccgcccaagg aaacgaagaa gtaagatcat gcggcaggtt gcgggcggcg cctcggcggc
     3061 gttgccgtgc gcaaccgcag ctagcggtag cagagaagga tacttgttgt gattcctagt
     3121 gacagcccca tactgcaaat tatctatatc ctggccgctg tcgccatcat catcggcctg
     3181 aagcggttgt cgtcgccggc cacggcgcgg accggcaacc tgatcgcggc ggtgggcgtc
     3241 gggattgcgc tgctggtgac actgttcacc agcgacatcc atgattacct gtggatcttc
     3301 atcggcgtgc ccatc
//
LOCUS       DASUYC010000424         3795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1551907, whole genome shotgun sequence.
ACCESSION   DASUYC010000424 DASUYC010000000
VERSION     DASUYC010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1551907"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="VF276_06990"
     CDS             <1..629
                     /locus_tag="VF276_06990"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX6606646.1"
                     /translation="AVARAGLIAAVGYHWRALDTIPEVRHTLANRPARMVLGAWHDAT
                     PPPEWWRHQATGGGQMVEQATHLFDLARLLVGEASVTAGTATRYGQAAYPDADVADVS
                     AALLRFDTEATGVFTATCLLGGVAAAQVQIVCEGLSITITQRSVLYDWGRERREVLTR
                     NDPFVDEDRAFLQAVQRNEPGLLVSSYADALRTHRLCFDVQAASGSRT"
     gene            778..1812
                     /locus_tag="VF276_06995"
     CDS             778..1812
                     /locus_tag="VF276_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX6606647.1"
                     /translation="MTATLHDVAAKAGVSAITVSRALNNSGYVSSHTRQRILDAATEL
                     NYVPNALASSLRSQRTQLLALLLTDVTNPFWTTVARGVEDTAIDAGYAVILCNTDEDP
                     TKEARYLDLLLRRRIDGLLIAPTVESTELLRTLKRRKVPFVLIDRQVSGLSADIVRGD
                     SYTGAYRLTAHLLATGYRQIAMIGGPATVSTGEDRVRGYLTALQDAGVAPDEGLIRRG
                     PYRQAWGYAAMQDLLASGRRPAAIFAANNVIALGVLEALRAHDLRVPEDVAVVSFDDF
                     PPSVGAAPFLTAAVQPAADLGRQAMRLLLERLAYPDGEPRDIVLPTELVVRQSCGCAP
                     PAPSAPGSGG"
     gene            2151..>3795
                     /locus_tag="VF276_07000"
     CDS             2151..>3795
                     /locus_tag="VF276_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6606648.1"
                     /translation="MKTKLFSCIAVFTVFSLLLAGCGTETATNTPVAAPTNTTAAASA
                     PTNTTAPAAPTDTTAPAAPAATNTTAPAAPAATNTTAPAASGGGSNQFAGVTVNVLTF
                     TGPQIAEPLQRRAPDFAALTGAKINVITIPMADLYQKILTDLATKTNSYQAFVFDPQW
                     LGDFVTPGYLEDITDRVKADTALQWDDVGPFFRDFSATYQGRTYTIPLDGDFHMIYYR
                     SDLLAKDGLQPPATWDDYLAIAKKYQGQDLNGDGTPDYGSCISMKRNAQAYWFIMSVA
                     GAYLQSKGTNQGAFFDTDTMKPLTNNDAFAKALDTYKAMLQYAPPDQLNLDVGDTRGL
                     FTSGRCALSMDWGDIGTLAIDPKTSKVQDKVGAAILPGSKEVLDRATGKLVPCDATTC
                     PYATNGVNHAPYAAFGGWSGAINAADDPKVKDAAYAFLSYMSQPKQSGEDVTLGKTGF
                     NPYRLSHFQNLDLWEKAGMSEAAAKLYLGAIQDSLNSPNMVLDLRVPNSNQYQGVILD
                     KAVSQFLAGEIGRDEAMQQITDGWEETTNQAGRDKQLAAY"
BASE COUNT          684 a         1294 c         1209 g          608 t
ORIGIN      
        1 aggccgtggc gcgcgccggg ttgatcgccg ccgtgggcta ccactggcgc gcactggaca
       61 cgatcccgga ggtgcggcac accctggcga accggcccgc gcgcatggtg ctgggcgcct
      121 ggcatgacgc cacgccgccg ccggagtggt ggcgccacca ggccaccggc ggcgggcaga
      181 tggtcgagca ggcgacgcac ctcttcgacc tggcccggct gctggtgggc gaggccagcg
      241 tgacagcggg cacagccacg cgctacgggc aggccgccta ccccgacgcc gacgtggccg
      301 acgtgagcgc ggccctgctg cgcttcgata cggaagccac gggggtgttc accgccacct
      361 gcctgctggg cggggtggcc gccgcccagg tgcagatcgt ctgcgagggc ctgtcgatca
      421 cgatcaccca gcgcagcgtg ctgtacgact ggggccgcga gcgccgtgag gtgctgaccc
      481 gcaacgaccc gttcgtggac gaggaccgcg cctttctcca ggccgtccag cgtaacgagc
      541 cgggtctcct cgtgagcagc tacgccgacg ccctgcgcac ccaccggctc tgcttcgacg
      601 tgcaggccgc ctccggctcc cgcacatagg aggccccgcc caccgggcca ctaataggtt
      661 gggttaccct attgcattcg gtgaaaagct atgctagaat gtggtaacgt taacatgaca
      721 acgttaacat aaacatctga ccgctgttca cgatacagtc gccaggaagg gcggggtatg
      781 acggcgacgc tgcatgatgt cgcggccaaa gcgggcgtct cggccattac cgtgtcgcgg
      841 gcgctcaaca acagtggcta tgtcagttcg cacacgcgcc agcgcatcct ggatgcggca
      901 accgaactca actacgtccc caacgctctt gccagcagcc tgcgctccca gcgcacccag
      961 ttattagccc tgttgctgac cgacgtcacg aatcccttct ggacgaccgt ggcgcggggt
     1021 gtggaagaca cggctatcga cgcaggctat gcggtcatcc tgtgtaacac cgacgaagac
     1081 ccgaccaagg aggcccgcta cctcgatctg ttgctgcgcc ggcggatcga cggcctgctc
     1141 atcgcaccca ccgtcgaaag cacggagttg ctgcgcacct tgaagcgccg caaggtgcct
     1201 ttcgtcctga tcgaccggca ggtgagtggg ctgagcgccg atattgtgcg cggagacagc
     1261 tacaccggcg cgtaccggct caccgcgcat ctgctggcga cgggctaccg gcagatcgcc
     1321 atgatcggcg gaccggcgac ggtgtcaacc ggggaagatc gggtgcgagg ctacctgacc
     1381 gctttgcaag atgcgggggt cgcgcctgac gaggggttga tccggcgcgg cccctaccgg
     1441 caagcctggg ggtatgcggc gatgcaagac ctgctggcga gcgggcggcg ccccgcggcg
     1501 attttcgcgg ccaataacgt gatcgcgctc ggcgtgctgg aagcgttgcg ggcccacgac
     1561 ctgcgcgtgc cggaggatgt ggctgtggtc tcgtttgacg actttccgcc ctcggtgggc
     1621 gccgcgccgt tcctgaccgc cgccgtgcag cccgcggcgg atctgggccg gcaggcgatg
     1681 cgcctgctgc tggagcggct ggcgtatccc gacggggagc cgcgggatat tgttttgccg
     1741 acagaattag tcgtgcgcca atcctgcggc tgtgcgccgc ccgcgccgtc cgcgcccggc
     1801 agcgggggtt agcgcgtggc gaccgagagg cccgattccc gctcggcagc gtagccggcg
     1861 atctcgctac tgctcaacgg cttcatccta gctgcgactc gcgtctatcc ggcatcgcgc
     1921 tctttgtttg ggcgtggaac tatcggatta tacgtcgcac tcctccgccc ctcccgttgg
     1981 ttcccgccca tatggtaacc gccttgcccg catgggccac gccagaaagg agaccttgcc
     2041 gaccctgaag aacgtgtggc gtggggcacg ccgtgccggg tcgccggtgg cgacgaccgc
     2101 ggctattcct attcacgcga acaggtgcga gattcatagg aggagagcga atgaagacca
     2161 agctgtttag ctgcatcgcg gtctttactg tgttttccct gctgctggca gggtgcggca
     2221 cagaaaccgc gacaaacacg cccgtcgcgg ctcccaccaa caccaccgct gctgcaagtg
     2281 ccccgaccaa tacgaccgct ccggcggcgc ccaccgacac gacggcgccc gcggcgcccg
     2341 ccgccacgaa tacgaccgcc ccggcggcgc ccgccgcgac caatacgacc gccccggcgg
     2401 ctagtggcgg cggcagcaac cagttcgccg gggtcacagt gaatgtgctg acattcaccg
     2461 gcccgcagat cgccgagccg ttgcagcgcc gcgcgcccga tttcgccgcg ctcaccggcg
     2521 ccaagatcaa cgtcatcacc attccgatgg ccgatctcta tcagaagatc ttaaccgacc
     2581 tcgccaccaa gaccaacagc tatcaggcct tcgtctttga cccgcagtgg ctgggcgact
     2641 tcgtcacgcc cggctacctc gaagacatta ccgatcgggt gaaagccgac accgccttgc
     2701 agtgggatga cgtcggcccc ttcttccgtg atttcagcgc cacctaccag ggccgcacct
     2761 ataccattcc gctcgacggc gacttccaca tgatttacta tcgcagcgac ctgcttgcca
     2821 aggacgggct ccaaccgccc gccacctggg acgattatct ggctatcgcc aagaagtacc
     2881 aggggcagga tctcaacggc gacggcacgc ccgattacgg ctcctgcatc tcgatgaagc
     2941 gcaacgcgca agcgtactgg ttcatcatgt cggtggccgg cgcctacttg cagagcaagg
     3001 gcaccaacca gggcgccttc ttcgataccg acaccatgaa accgctgacc aacaacgacg
     3061 ccttcgccaa ggccctcgac acgtacaagg ccatgctgca atatgccccg cccgatcaac
     3121 tcaacctgga cgtgggcgac acgcgcggcc tgttcacctc gggccgctgc gcgctctcca
     3181 tggactgggg cgacatcggc acgctggcaa tcgatcccaa gaccagcaag gtgcaggaca
     3241 aagtgggcgc cgcgatcttg cccggatcga aagaagtgct ggaccgggcc acgggtaagc
     3301 tggtgccctg cgacgcgacg acgtgcccct acgccaccaa tggcgtcaac catgcgccgt
     3361 atgccgcctt cggcggctgg tccggggcga tcaacgcggc ggatgacccc aaggtcaagg
     3421 acgcagccta tgccttcctc tcgtatatga gccagcccaa gcaatcgggg gaggacgtga
     3481 cgctgggcaa gaccggcttc aacccctatc gcctctcgca cttccagaac ctggacctgt
     3541 gggagaaggc gggcatgagc gaggcggcgg cgaagctgta cctgggcgcc atccaggaca
     3601 gcttgaacag cccgaacatg gtgctggacc tgcgggtgcc caactcgaac cagtatcaag
     3661 gcgtgatcct ggacaaggcc gtgtcgcagt tcctggcggg ggagatcggc cgcgacgagg
     3721 ccatgcagca gatcaccgac ggctgggaag aaacgaccaa ccaggcgggc cgcgataagc
     3781 aactggccgc gtata
//
LOCUS       DASUYC010000425         2019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_174386,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000425 DASUYC010000000
VERSION     DASUYC010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174386"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="VF276_07005"
     CDS             <1..728
                     /locus_tag="VF276_07005"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEX6606649.1"
                     /translation="LGVAEPVSDFRVSSTADVRARFAEARQHGAKIVLNHPFHVCDSP
                     GCHWDWGWDVDFDWYEVWNGPWRSVNLEALAWWQGQLAQGRRLVAVGGSDTHRPDPYT
                     GHGKPTTWVWATAATAPAILDAIGQGHVFISQLPEGPRIDLRCSAFMMGDEVPAGGAG
                     DGAPPLTVKVDGLQAGDQVRVLSEQGIAQEWTAGPGDTALRREWPAPPRRFYRVEIWR
                     YATAAGQPVVAALSNPIYFAPGP"
     gene            836..>2019
                     /gene="hemG"
                     /locus_tag="VF276_07010"
     CDS             836..>2019
                     /gene="hemG"
                     /locus_tag="VF276_07010"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HEX6606650.1"
                     /translation="MVDHTLDVAVVGGGLAGLATAYFLQQQARAAGRPVRLVVLEQEA
                     RPGGKLITDRVDGFIIDGGPDSFGPAPPDALALSRDLGLEDQLIGSADAQNRIYVLAG
                     GKLHAPPAGLVGVVPTDRGALARSSLLGALGQLRMAREVSLATQPGADDESIAAFVYR
                     RFGQEAVEKLARPLLASPFLGEPEWLSMRLLFPDYLDLEREHGSLILGLQARRPPAEL
                     DQGPGALLSFREGMQTLVEALAAHLDPESLHLGRSITQISRAAYTDAPYTLRTKEGRG
                     YSARRVVLAVAAPVAANLLPDRAAALASALRQIRYLSSATVALGYRKDEVGHPLDGFG
                     FAAAAREQTPLLTCTWTSTKFPDHAPAGHVLLRAVVAGSAGAPALDLLDLELVGLVRA
                     ELG"
BASE COUNT          268 a          726 c          728 g          297 t
ORIGIN      
        1 tattgggcgt ggccgagccg gtcagcgact tccgcgtgtc cagcaccgcg gacgtgcggg
       61 cgcgcttcgc ggaggcgcgg caacacgggg cgaagatcgt cctgaaccac ccgttccatg
      121 tctgcgactc gccgggctgc cactgggatt ggggctggga cgtggacttc gactggtatg
      181 aagtctggaa cggcccctgg cgctccgtaa acctcgaagc gctggcctgg tggcaggggc
      241 aactggcgca gggccgccgc ctggtggctg tcggcggcag cgacacccac cggcccgacc
      301 cgtataccgg ccacggcaag cccacgacct gggtctgggc cacggcggcc acggccccgg
      361 cgattctcga cgctatcggc caggggcacg tgtttatcag ccagttgccg gagggtccgc
      421 ggatcgacct gcgctgcagc gcgttcatga tgggcgacga agtgcctgcc ggcggggccg
      481 gggacggcgc gccgccgctg acggtcaagg tggatgggct gcaggcgggc gaccaggtgc
      541 gggtgctgtc ggaacagggg atcgcgcagg aatggaccgc cggccccggc gacacggcgc
      601 tgcggcgcga gtggccggcc ccgccgcgcc gcttctatcg cgtcgagatc tggcgctacg
      661 cgacggccgc cgggcagccg gtggtggcgg cgctgagcaa tccgatctac ttcgcgcccg
      721 gcccgtaggg cgccccgcgg gccgagtgcg ctgcgccttg ctgggcaccg gaccttaccg
      781 tatggtatag tggtcctatg atagcattgt atccatacga gatcggcccc cggtgatggt
      841 ggaccatacc ctcgacgtgg ccgtggtcgg cggcgggctg gccgggctgg caacggccta
      901 ctttctccag cagcaagccc gcgcggcggg ccggccggtg cggctggtgg tcctcgaaca
      961 ggaggctcgc cccggcggca agctgatcac cgaccgggtg gacggcttca tcatcgacgg
     1021 cgggcccgac tccttcggcc ccgcgccccc cgatgccctg gcgctcagcc gggacctggg
     1081 cctggaagat cagctcatcg gcagcgcgga tgcgcagaac cgcatctatg tgctcgcggg
     1141 cgggaagctg cacgcccccc cggcgggcct cgtgggcgtc gtccccaccg accggggcgc
     1201 gctggcccgc agcagcctgc tcggtgccct gggccagctg cgcatggcgc gcgaggtgtc
     1261 cctggcgacc cagcccggtg ccgatgacga gagtatcgcc gctttcgtct accgccgctt
     1321 cggccaggaa gccgtcgaga aactggcccg tcccctgctg gcgagcccat ttctgggcga
     1381 acccgagtgg ctgagcatgc gcctgctgtt ccccgactac ctcgacctgg agcgggagca
     1441 cggcagcctt atcctgggcc tccaggcccg gcgcccgccc gcggagctgg accaggggcc
     1501 gggcgccttg ctgtctttcc gcgagggcat gcaaacactg gtcgaggcgc tggccgcgca
     1561 cctggacccg gagagcctgc acctgggccg ctcgattacc cagatcagtc gggcagccta
     1621 cacggacgcc ccctacaccc tgcgcacgaa agaaggccgt ggctacagcg cgcgccgggt
     1681 ggtgctggcg gtggccgcgc cggtggccgc gaacctgttg ccggaccgcg ccgccgccct
     1741 ggcctccgcc ctacgccaga ttcgctacct ctccagcgcg actgtggcgc tcggctaccg
     1801 caaggacgag gtcgggcatc cgctcgacgg cttcggcttt gccgccgccg cccgcgaaca
     1861 gaccccgctg ctcacctgca cctggacctc gaccaagttc cccgaccacg ccccagcggg
     1921 ccacgtgctg ttgcgggccg tcgtcgcggg cagcgccggg gccccggccc tggacttgct
     1981 cgacctggaa ctggtcgggc tcgtgcgggc cgagttggg
//
LOCUS       DASUYC010000426         3523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1663887, whole genome shotgun sequence.
ACCESSION   DASUYC010000426 DASUYC010000000
VERSION     DASUYC010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1663887"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VF276_07015"
     CDS             complement(<1..400)
                     /locus_tag="VF276_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEX6606651.1"
                     /translation="MESKAKLAGHPIHQMLIVFPLGLLVMALIFDILYFITGNAGFAT
                     AAFWDIPAGIIGGLVAAVFGFIDWLSIPAGTRAKRVGLLHGGGNVVVVVLFAISWLLR
                     WGAPDGRPDLVAFIPALLAVCLGAVTGWLGG"
     gene            543..1178
                     /locus_tag="VF276_07020"
     CDS             543..1178
                     /locus_tag="VF276_07020"
                     /inference="COORDINATES: protein motif:HMM:NF036806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEX6606652.1"
                     /translation="MSSTRWDQRFFASTRGRIVSLLRRTSRTVDELAGALDLTDNAVR
                     AHLAALERDGLVEQRGVRRGAGKPAYSYELTPEAERLFPKPYSLVLRELLTTLAGELG
                     SEETEAVLRATGRRLAAGQYAPGTDLHARCEGAVALLNAWGGLAETELAEDGTGYVIR
                     GYSCPLASVVAGRPDTCRLVEELLTAYLGTPVHEQCTHGDSPRCCFAVPQA"
     gene            1656..2822
                     /locus_tag="VF276_07025"
     CDS             1656..2822
                     /locus_tag="VF276_07025"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX6606653.1"
                     /translation="MAEVIDRVELSGIVKVRDVILKLEKPYRLESGEPSFDVPAHIKE
                     AMVRALRDNQTHYVASAGIAELRKAIVTKVRTSNGIPMESSAETIVTNGGMHALYTLF
                     RALLEPGDEVLIPDPTWTCVQHLITLCGGVVKRVPLHEERGWTFDPDEVRAAITPRTR
                     AFMINSPHNPTGGVLGRADLEAIAAIVADNPQVTVVSDEAYEHILYDGAQHISFASLP
                     GMYERTATVFTFSKSYAMTGLRLGYVVTPDRTTLDRMQKVVLYTANGVNSITQWGGVA
                     ALTGPQDCLAEFRQEYQARRDLFYDGLRDVPVFTGARPAGAFYAFLRIADGWTAPDGR
                     RDSWAMTEYLLQARVGSSPGIIFGPSGDNYLRFSTACTREDLTGALAAMRALFA"
     gene            3028..>3523
                     /locus_tag="VF276_07030"
     CDS             3028..>3523
                     /locus_tag="VF276_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX6606654.1"
                     /translation="MPRMDTQKDGQEITRERLVELLNEDLAREYQAIISYVIYSQVLK
                     GAEYMSIASELEVHAGEELQHALTISKQIDYFGGMPTTTPKPVRTSEKAEDMLRFDLE
                     NENETVRNYRERVRQAEALGEFALAEDLREIVRQEQEHQIDLATALGIDVPKQGLGSE
                     AGKVH"
BASE COUNT          648 a         1189 c         1140 g          546 t
ORIGIN      
        1 cgccgcccag ccaccccgtc accgcgccga ggcatacggc gagcagggcc ggtatgaacg
       61 ccacgagatc gggccgtccg tcgggcgcgc cccagcgcag cagccagctt atggcgaaca
      121 acacgacgac gaccacattg ccgccgccgt gcagcaggcc gacgcgcttg gcccgcgtgc
      181 cggcggggat cgacagccag tcgataaagc cgaacaccgc cgcgaccaac ccgccgatga
      241 tgcccgccgg aatatcccag aaggccgccg tggcgaatcc ggcgttgccg gtgatgaaat
      301 acagaatgtc gaatatcagc gccatgacca gcagaccgag cgggaagacg atcagcatct
      361 gatggatcgg gtgcccggcc agcttggctt tgctttccat aacccttgct cctttcatgg
      421 atggcagccg ttcatacatc cactatagca ccgtttcctg agaaagtcaa taacttcttt
      481 gacttatttg acagaaccga tttttgctgt ataatgaagc tatagcgtga agggagtctg
      541 ccatgtcgtc aacgcgttgg gaccagcgat ttttcgccag cacacggggc cgcatcgtga
      601 gtctgctgcg gcggaccagc cggacggtcg atgagctggc cggtgccctc gatctcactg
      661 ataacgccgt gcgggcgcac ctggccgcgt tggaacgcga cggcctggtc gagcagcggg
      721 gcgtgcgccg gggcgccggc aagcccgcct actcctatga gctcacgccc gaggccgaac
      781 gactgttccc gaaaccctac agcctggtct tgcgcgagtt gctgaccacc ctggcgggcg
      841 aactgggttc cgaagagacg gaggcggtgc tgcgcgcgac gggtcgccgg ctggctgccg
      901 ggcagtatgc acccggcacg gatctgcacg cgcgctgcga aggcgccgtg gcgttgctca
      961 acgcctgggg cggactggcc gagacggaat tggccgagga cggcaccggc tacgtcatcc
     1021 gcggctatag ctgccccctg gcgtcggtgg tcgcgggccg gcccgacacg tgccggttgg
     1081 tcgaggagtt gctcacggcc tacctgggca cgcccgtgca cgagcaatgc acgcacggcg
     1141 actcgccgcg ctgctgcttc gccgtgccgc aagcgtaggc ggggccgtcg tttcgcgcag
     1201 gcgcgcgagc gcgggacttg acaggatcgg ctttctccga tagaatcact ctaattgaaa
     1261 acttcggcgc tgaatgagtg aagtacccgg accgcccagg aacagagagc tacactggct
     1321 gagacgtagc cctggatgcc cggcgaacag accctcagga gccgccgggc agaatgcacg
     1381 ccacccccgg cgtgttagta ggcccggccg agtcgctctc gttatcgagc ctttcgcctc
     1441 cagcccctgt atgggccggc gactgcaagg cgcgtccatc cgggcgcgcg aagcaaggtg
     1501 gcaccacgaa agcccagcat cgtccttgca acgagtgctg ggcatgttca ttttgtgcgc
     1561 tttgcagtgt cgcaagctcc cacaacgacc catccgggcg gatcgcgcca tgccggcggc
     1621 agccgggtgg gtacgcattg gaggtagaaa aggcaatggc agaagttatc gaccgcgtcg
     1681 agttgtcggg catcgtgaag gtgcgcgacg tgatcctcaa gctggagaag ccctaccgcc
     1741 tcgaaagcgg cgaacccagc ttcgatgtcc ccgcccacat caaagaggcc atggtgcgcg
     1801 ctctgcgcga taaccagacc cactatgtgg cgtccgccgg gatcgccgaa ctgcgcaagg
     1861 cgatcgtgac caaggtccgc accagcaacg gcatcccgat ggaaagctca gccgaaacca
     1921 tcgtcaccaa cggcggcatg cacgcgctgt acacgctgtt ccgcgccttg ctggagccgg
     1981 gcgacgaggt gctgatcccc gaccccacct ggacctgcgt gcagcacctg atcacgctct
     2041 gtggcggcgt ggtcaagcgc gtgccgctgc acgaagagcg cggctggacg ttcgaccccg
     2101 acgaggtacg cgccgcgatc acgccgcgca cgcgcgcctt catgatcaac tcgccgcaca
     2161 atccgaccgg tggcgtgctg ggccgcgccg acctggaggc catcgcggcg attgtcgcgg
     2221 acaacccgca agtgactgtc gtcagcgacg aagcctacga gcacatcctg tacgacggcg
     2281 cgcagcacat cagcttcgcc tcgctgcccg gcatgtacga gcggacggcg accgtgttca
     2341 ccttcagcaa gtcctacgcg atgaccgggc tgcgcctggg ctacgtcgtt acgcccgacc
     2401 gcacgaccct ggaccgcatg cagaaggtgg tgctctacac ggccaacggt gtcaactcga
     2461 tcacccagtg gggcggcgtg gccgcgctga ccgggccgca ggactgcctg gccgagttcc
     2521 gccaggagta ccaggcccgc cgcgacctgt tctatgacgg gttgcgggat gtgccggtgt
     2581 tcaccggggc gcgcccggcg ggggcgttct acgccttcct gcgcatcgcc gacggctgga
     2641 ccgcgccgga cgggcggcgc gactcctggg ccatgaccga gtacctgttg caggcgcggg
     2701 tcggctcgtc gccgggcatc atcttcggcc ccagcggcga caactacctg cggttcagca
     2761 ccgcgtgtac gcgcgaggat ctgaccggcg cgctggccgc catgcgggcg ctgttcgcct
     2821 agcgctctac ggcccatgcc accgcacacc acccacgtag ccggcgatca gccctggcgg
     2881 ctggtccccg gctacgtgcc gtttccggcg cccgccggat gaccttggcc cgcaaaatgc
     2941 agaggtagcc gcgattgccg gcgtgctggt tgccccgctg ccggccccga ttcatcaccc
     3001 cggccactgc atgccggtac aaggagtatg ccccgtatgg atacccagaa ggacggacag
     3061 gaaatcaccc gcgaacgcct ggtggaactg ctgaacgaag acctggcgcg cgaataccag
     3121 gccatcatct cgtatgtcat ctacagtcag gtgctgaaag gcgccgaata catgagcatc
     3181 gcctccgaat tggaggtcca cgccggcgaa gagctacagc acgcgctgac catctccaag
     3241 cagatcgact acttcggcgg catgcccacc accacgccca agccagtgcg cacctccgag
     3301 aaggccgagg atatgctgcg cttcgacctg gagaacgaga acgagacggt gcgcaactac
     3361 cgcgagcgcg tgcgccaggc cgaggcgctc ggcgagttcg ccctggccga ggacctgcgc
     3421 gagatcgtcc gccaggagca ggagcaccag atcgacctgg cgacggccct cggcatcgac
     3481 gtgcccaagc agggcctggg cagcgaagcc ggtaaggtcc acg
//
LOCUS       DASUYC010000427         2607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1580665, whole genome shotgun sequence.
ACCESSION   DASUYC010000427 DASUYC010000000
VERSION     DASUYC010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1580665"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VF276_07035"
     CDS             <1..209
                     /locus_tag="VF276_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX6606655.1"
                     /translation="AGDEVTEIVLDICDQYTIQADLFARAVLDDTPVPTPLADAVANM
                     RVIDAVRESARTGAWVTPGSGAGR"
     gene            complement(241..1497)
                     /locus_tag="VF276_07040"
     CDS             complement(241..1497)
                     /locus_tag="VF276_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX6606656.1"
                     /translation="MKITNVRAILTAPDGIALVVVKVETDQAGLYGLGCATFTQRPTL
                     VARAIEEYLQPLLQGRDPRNSEDLWHLMMVNAYWRNGPVLNNAVGGVDTALWDIKGKI
                     AGLPVYDLLGGKCREAALVYRHADGRDAQEVLDNVLRYRDAGYHAVRCQLGGYGGRVQ
                     DIRAATALPSRDAPQATHRQQTLDRLDSAFPGAYYDPDRYARSIPPVFEHVRAAVGFD
                     LLLLHDIHERLAPIDAIRLAKALEPYRLFFLEDPLAPEQSEWFRRLRDQCATPIAMGE
                     LHTHPLEWRALITGQLIDFIRSHISAIGGLTPARKLAALGEAFGVRTAWHGPGDVSPV
                     GHAANLHLDLACHNFGIQEVISFGEALYEVFSGIPELRNGYLWANDRPGLGVDIDEAQ
                     AAKYPLSVQPIGWTQSRRPDGTLWTP"
     gene            complement(1522..2331)
                     /locus_tag="VF276_07045"
     CDS             complement(1522..2331)
                     /locus_tag="VF276_07045"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX6606657.1"
                     /translation="MDLQAVFESIPQRYPELRGQVAVVTGSARGIGKGIALRLAREGM
                     RVVINSTTVAEVAATAAEFRGLGAEVVEHVGDVGQAADVGALFQATLDAFGGVDLLVN
                     NAGLLGSPPFGELAEARFDRVLRTNVKGPFLCARRAVDLMRPRGRGTIVHISSVGGLR
                     VHQPGVPYDVTKAALDGMTRAMGVELADDGIRVNAVAPGAIWTESRPPRDDPAMQAVA
                     ERIPMRRRGDTLEIGSVVAFLASADASYITGQVIYVDGGITAQLYPRGQFI"
BASE COUNT          381 a          895 c          929 g          402 t
ORIGIN      
        1 aggccggcga cgaagtgacc gagattgtgc tggacatctg cgaccagtac accatccagg
       61 ccgacctgtt cgcccgcgct gtgctggacg ataccccggt gcccacgccg ctcgccgatg
      121 cggtcgccaa catgcgcgtc atcgacgccg tgcgcgagag cgcccgcacc ggcgcctggg
      181 tgacgccggg cagcggcgcc ggcaggtagc ggtccgcgcg ctacctcccg ccccggcggc
      241 tcacggtgtc cagagcgtgc cgtcgggccg gcggctctgt gtccacccga tcggctgcac
      301 gctgagcggg tactttgcgg cctgggcctc gtcgatgtcc acgcccaggc cgggccggtc
      361 gttggcccac aggtagccgt tgcgtagctc ggggatgccg gagaagacct cgtatagcgc
      421 ctcgccgaag gagatcacct cctggatgcc gaagttgtgg caggccaggt ccaggtgcag
      481 gttggccgcg tggcccaccg gggagacatc gcccggccca tgccacgccg tgcgcacgcc
      541 gaacgcctcg cccagcgccg ccagcttgcg cgccggagtc agcccgccga tagccgagat
      601 gtggctgcgg atgaagtcga tcaactgccc ggtgatcagc gcccgccact ccagggggtg
      661 cgtgtgcagc tcgcccatgg cgatgggcgt ggcgcactga tcgcgcaggc ggcggaacca
      721 ctcgctctgc tcgggcgcga gcggatcttc caggaagaac aggcgatacg gttccagggc
      781 cttcgccaac cggatcgcgt cgatgggcgc cagccgctcg tggatgtcgt gcaacagcag
      841 caggtcaaaa ccgacggcgg cgcggacgtg ctcgaacacg ggtgggatcg agcgggcgta
      901 gcggtcggga tcgtagtagg ctccgggaaa ggcgctgtcg agccggtcca gcgtttgctg
      961 ccggtgggtg gcctgcggcg cgtcgcggct gggcaacgcc gtcgccgcgc ggatgtcctg
     1021 cactcgcccg ccgtagccgc ccaattggca gcgcacggcg tgatagcccg cgtcgcggta
     1081 gcgcaggacg ttgtcgagca cctcctgggc gtcgcgcccg tcggcgtggc ggtagaccag
     1141 ggcggcctcg cggcacttgc cgcccaacag atcatagacc ggcagcccgg cgatcttgcc
     1201 cttgatgtcc cacagcgccg tatccacccc gccgacggcg ttgttcagca ccgggccgtt
     1261 gcgccagtag gcgttgacca tcatcaggtg ccagaggtct tcgctgttgc gcgggtcgcg
     1321 gccctgcagc aagggctgga ggtactcctc gatggcccgc gcgacgagcg tgggccgctg
     1381 ggtgaaggtg gcgcagccca ggccatagag gcccgcctgg tcggtctcga ccttgaccac
     1441 gaccagcgcg atgccgtcgg gcgcggtgag gatggcgcgc acgttggtaa tcttcatgct
     1501 tccctcctgg cgggcgccgg ttcagatgaa ttggccgcgc gggtacaact gggccgtgat
     1561 cccaccgtct acgtagatca cctggccggt aatataggag gcatcggcgg acgccaggaa
     1621 ggcgacgacc gatccgatct ccagcgtatc gcccctgcgc cgcatgggga tcctttcggc
     1681 gaccgcctgc atggcagggt cgtcgcgcgg cgggcggctt tcggtccaga tggcgccggg
     1741 ggccacggcg ttcacgcgga tgccgtcgtc ggccagttcg acgcccatgg cgcgggtcat
     1801 gccgtcgagc gccgccttgg tcacatcgta gggcacgccg ggctggtgca cgcgcagccc
     1861 gcccaccgag ctgatgtgga cgatagtgcc ccgcccgcgc gggcgcatca gatcgacggc
     1921 ccggcgcgcg cagaggaacg gccccttgac gttggtgcgc aggaccctgt cgaagcgcgc
     1981 ttccgccaac tccccaaacg gcggcgaccc cagcaggccg gcgttattga ccagcagatc
     2041 gaccccgccg aaagcgtcga gcgttgcctg gaagagcgca ccgacatcgg cagcctggcc
     2101 cacatcgccc acatgctcga ccacctccgc gcccaggccg cggaactcgg cggccgtcgc
     2161 cgccacttcg gcgaccgtcg tgctgttgat gacgacgcgc atcccctcgc gggccagccg
     2221 cagcgcgatg cccttaccga tgccgcgcgc cgagccggtg acgaccgcca cctggccgcg
     2281 tagctcggga tagcgttggg ggatcgattc gaagacggcc tgtaaatcca tgggcgcgca
     2341 cctcctcagt ggcttttccg cgataccggg gcaaatcaat ggccggatcc gccgtcggca
     2401 gatccggcca gggcctcatt atagcaccag aatcgatcat ccgggccgct atcggcgcat
     2461 cctgcgcgcc ttagcgcgac gaccgcgttc gggtgagagg ctgttgcggt gtggtgcggg
     2521 aggtcacggc cggggtcgtg gcggtgggta cgggtttgct ggtggcggtg gccgccggcg
     2581 cagtcgcggt ggccgtggcc tccggtt
//
LOCUS       DASUYC010000428         2662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_533160,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000428 DASUYC010000000
VERSION     DASUYC010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533160"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VF276_07050"
     CDS             complement(<1..518)
                     /locus_tag="VF276_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006633093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HEX6606658.1"
                     /translation="MAQIISLHSFRGGTGKSNTTANLATLLAGEGRRVGVIDTDIQSP
                     GIHVLFGLRGPQITYALNDYLWGRCSVADIARPVLQDVLPDRLFLIPSSIKADDIVQV
                     IRQGYDVRRLTMAFRELIDHLHLDVLLIDTHPGLNEETLFALAISTTLAIVLRPDQQD
                     YEGTGVAVEVARS"
     gene            complement(521..1330)
                     /locus_tag="VF276_07055"
     CDS             complement(521..1330)
                     /locus_tag="VF276_07055"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX6606659.1"
                     /translation="MLTDRDKALLLRMVPFFSALPDEVLVDLAPRTREVLVPEGQAVV
                     HKGEPGDCMYIIIRGEARVHDGEHQMNYLRRGDIFGETAALDGLPRVATVTAVFELAL
                     LEIDRTALFALMRESIAAMEGIVRGLTRWVRPTLHQSSADYHYIQAMTRLTAAAAQVE
                     AGVYEPEGLDRVAARQDDLGQLARLFQRMIHEVYTREQALQQQVVELRIEIDRAHQAR
                     QVAQIVDTGYFQNLRDQARDLRQMLGAAAGPAPPPAARPPDELVVPSPREP"
     gene            complement(1447..>2662)
                     /locus_tag="VF276_07060"
     CDS             complement(1447..>2662)
                     /locus_tag="VF276_07060"
                     /inference="COORDINATES: protein motif:HMM:TIGR04215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor A family protein"
                     /protein_id="HEX6606660.1"
                     /translation="ALTDASDSIADMAAGFSYAFDCGSGYGAYGPAASATCVAPGGFG
                     SFTVNGKIRDKDGLESEYSAFVQVVAVLSTPTPSPTSTIPPATGSATPTAPAAVTATP
                     TRTPTTTPTQTPTPTRTATPTATDTPVPTATATLTAPPTRTATATATPGLNCAPLGPA
                     ADFNLFSLGAINAANSSTGGRMAGAGPVTLMNYRVGAGLPNSHGTRDDLISGTDLTFT
                     NGQVARGNAVYGGTGRFTNVSFPNGSARQGNVIDFPAARQFLQNASTTWATLATNGTI
                     TWQRGQYTLTGSSNTLNVFNISGANLAQATSLTVTAPAGSTVLVNVDGSSLTLANFGI
                     TIRGTDQQKVLYNFAAATSLNAGNLGVPGSILAPRAAAGFTNDQVNGNLIAASFTGPA
                     ALNQQPFTGCLP"
BASE COUNT          397 a          838 c          960 g          467 t
ORIGIN      
        1 gaccgggcca cctccaccgc cacgcccgtg ccctcgtaat cctgttgatc ggggcgcagc
       61 acgattgcca gtgtggtcga gatcgccagc gcgaacagcg tctcttcgtt caggccaggg
      121 tgggtatcaa tcaggagcac atccaggtgc aggtggtcga tcaattcgcg aaacgccatg
      181 gtcaagcgcc gtacatcgta gccctggcgg atgacctgca caatgtcgtc ggctttgata
      241 ctcgatggaa tcaggaagag ccggtcgggc agcacgtcct gtagcacggg ccgggcgatg
      301 tcggcgacgg agcaccggcc ccacaggtag tcgttgaggg cgtaggtgat ctgcggcccg
      361 cgcagcccga agagcacatg gatgccgggt gattggatgt ccgtgtcgat gactcccacc
      421 cgccggcctt cccccgcgag cagcgtggcg aggttggcgg tcgtgttgga cttgcccgtg
      481 ccgccgcgaa aggagtgcag ggaaatgatc tgggccatgc ttatggctcc cgtggggagg
      541 ggactacgag ttcatccggg gggcgggccg cggggggcgg ggccggcccg gcggctgccc
      601 ccagcatctg gcgcagatcg cgggcctggt cgcgcaggtt ctggaaatag ccggtatcca
      661 caatctgcgc gacctgacgg gcctggtgcg cgcggtcgat ctcgatccgc aattccacca
      721 cctgctgttg cagcgcctgc tcgcgggtat agacctcgtg gatcatgcgc tggaacaggc
      781 gggccagttg gcccaggtca tcctgccgcg ctgccaccct gtccaaccct tcgggctcat
      841 agacgcccgc ctcgacctgg gccgccgccg cggtcagccg cgtcatggcc tggatatagt
      901 gataatccgc gctgctctgg tggagcgtgg gccgcaccca gcgggtcagc ccacgcacga
      961 tgccctccat ggctgcgatg ctctcgcgca tcagggcgaa cagggcggtc cggtcgatct
     1021 ccagcagtgc caactcgaac accgccgtca ccgtcgccac gcgcggcagc ccgtccaggg
     1081 ccgccgtttc gccgaagatg tcgccccggc gcagataatt catctggtgc tcgccgtcat
     1141 ggacgcgcgc ctcgccgcgg atgatgatat acatgcagtc gcccggctcg cctttgtgga
     1201 ccacggcctg accctcagga accaatacct cacgggtccg cggggcaagg tccaccagca
     1261 cttcgtcggg cagcgccgag aagaagggta ccattcggag tagcagcgcc ttgtctctgt
     1321 cggtcaacat ttctggcttt gccctcctcg ctatggatca gcggcgacgc gataccgggc
     1381 ctcattatag cacaacggct gcagcctggg gccacggcga aaacccgtct cgtccacggc
     1441 ggcggctcag ggcaggcagc cggtgaacgg ctgttggttc agcgccgccg ggccggtgaa
     1501 agacgccgcg atcaggttgc cgttgacctg atcgttggtg aagccggcgg cggcgcgggg
     1561 ggccaggatg ctgcccggca cgccgagatt accggcgttc agactggtag cggccgcgaa
     1621 gttgtagagc accttctgct ggtcggtgcc tctgatcgtg atgccgaagt tcgccagcgt
     1681 gaggctgctg ccgtccacgt tcaccagcac cgtggacccc gccggggccg tgacggtgag
     1741 gctcgtcgcc tgggccaggt tggccccgga gatgttgaag acgttcagcg tgttgctgct
     1801 gccggtcagg gtgtactgcc cgcgctgcca ggtgattgtc ccgttcgtcg ccagcgtcgc
     1861 ccaggtggtc gaggcgtttt gcaggaattg ccgggcggcg gggaagtcaa tcacattgcc
     1921 ctggcgcgcg ctgccgttgg ggaagctgac gttggtaaag cggcccgtac cgccgtagac
     1981 cgcgttgcct cgggcgacct ggccgttggt gaaagtcagg tcggtgccgc tgatcaggtc
     2041 gtcgcgggtg ccgtgcgaat tgggcaggcc ggcccccacc cggtagttca tcagcgtgac
     2101 cggtccggcc cccgccatgc gcccgccggt ggacgagttg gcggcattga tcgcgccaag
     2161 actgaacagg ttgaagtcgg cagccggccc cagtggcgcg cagttcagcc ccggcgtagc
     2221 cgtcgcggtg gctgtccgtg tcggcggcgc tgtgagcgtc gccgtggcgg tggggacggg
     2281 tgtgtcggtg gccgtgggcg ttgccgtgcg ggtgggcgtg ggcgtctggg ttggggtggt
     2341 ggtcggcgtg cgagtgggtg tggccgtcac ggcggcgggt gccgtgggcg tcgcgctccc
     2401 ggtggcgggt gggatggtgg atgtcgggga aggcgtaggc gtggacagca ccgccacgac
     2461 ctgcacaaag gcgctatatt ccgactccag gccatcctta tcgcggatct tgccgttgac
     2521 ggtgaagcta ccgaagccgc ccggcgccac acacgtggcg gaagcagccg ggccgtaggc
     2581 gccgtagccg ctcccgcagt cgaaggcgta gctgaacccg gcggccatgt cggcgatgga
     2641 atcgctggcg tcggtgagcg cg
//
LOCUS       DASUYC010000429         2603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_991848,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000429 DASUYC010000000
VERSION     DASUYC010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991848"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="VF276_07065"
     CDS             complement(<1..998)
                     /locus_tag="VF276_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004576445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HEX6606661.1"
                     /translation="MSDRYELILTCPKSLESLLLEEASGLGLEEAREQTAAVRGFAPI
                     EVGYRLCLWSRLANRVLLVLRRFPVGNAEELYQGVLAVDWAEHLDPAGTLAVEFSGHG
                     AGIDNTHFGALKVKDAIVDKLRTAEGIRPSIDKVDPHLRVHLRLDRGEAVLSLDLSGH
                     SLHQRGYRLQQGAAPLKENLAAAILIRAGWPRIAAAGGALADPMCGVGTFLVEAAMMA
                     ADLAPNLKRERWGFSNWLGHVPAIWKKLHAEAEQRAAAGLAKPPLWIRGYEADPRLIQ
                     PARNNIERAGLGEWVKLYQGELATFEPRPDKNQTGLIVSNPPYGERLGDEASLLYL"
     gene            1404..1619
                     /gene="rmf"
                     /locus_tag="VF276_07070"
     CDS             1404..1619
                     /gene="rmf"
                     /locus_tag="VF276_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002553055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HEX6606662.1"
                     /translation="MRRLKRDPLERAFLRGYQHGIHGKSRDMCPFTHPTTRQAWINGW
                     REGRGDNWDGLTGTAGIHRLNELHAVG"
     gene            complement(1720..>2603)
                     /locus_tag="VF276_07075"
     CDS             complement(1720..>2603)
                     /locus_tag="VF276_07075"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEX6606663.1"
                     /translation="MGLQFPNPVGLAAGLDKTGDAIDGFAQLGFGFVEIGTVTPRPQP
                     GNPKPRLFRLPEATAIINRMGFNNHGVDHLLARVRAAKFKGVLGINIGKNFDTPVERA
                     VDDYLIGLDKVYAHASYVTVNVSSPNTPGLRSLQFGDQLKQLLEALRLRQEDLAVQHG
                     KRVPLAIKIAPDMSDEETVQVAQALVETGMDAVIATNTTLGREGVENLPFGNEAGGLS
                     GAPVRDKSTHTVRVLAGELKGRLPIIAVGGIIEGAHAAEKIAAGASLVQIYSGFIYKG
                     PALIREAVDASAALPRT"
BASE COUNT          458 a          879 c          850 g          416 t
ORIGIN      
        1 tagagataga gcaggctcgc ctcgtcgccc aggcgctcgc cataaggcgg gttgctgacg
       61 atcaggccgg tctgattctt gtccgggcgc ggctcgaagg tggccagctc accctggtac
      121 agcttgaccc actcgccgag cccggcgcgc tcgatgttgt tgcgcgccgg ctggatcagc
      181 cgcggatcgg cctcgtagcc gcgaatccac agcggcggct tggccaggcc ggcggcggcg
      241 cgttgctcgg cctcggcgtg cagcttcttc cagatcgccg gcacatggcc cagccagttg
      301 gagaaacccc agcgctcgcg cttgaggttg ggcgccagat cagcggccat catcgccgcc
      361 tcgaccagga aggtgccgac cccgcacatc gggtcagcca gcgcgccgcc cgcagcggca
      421 atacgcggcc agccggcgcg gatcaggatg gcagcggcca ggttctcctt gagcggcgcg
      481 gcgccctgct gcaggcgata gccgcgctgg tgcaggctgt gcccggagag atccagcgac
      541 agcaccgcct cgccgcgatc caagcgcagg tgcacgcgca ggtgcgggtc gaccttgtcg
      601 atcgacgggc ggatgccctc cgccgtgcgc agcttgtcga cgatggcgtc cttgaccttg
      661 agggcgccga agtgggtatt gtcgatgccg gcaccgtggc cgctgaactc cacggccagg
      721 gtaccggccg ggtcgagatg ctcggcccag tccaccgcca acacaccctg gtaaagctct
      781 tcggcgttgc ccaccggaaa acgccgcaac accagcagca cgcggttggc caggcgcgac
      841 cagaggcaca ggcgatagcc cacctcgatg ggtgcgaagc cacgcacggc ggcggtctgc
      901 tcgcgggcct cctccagccc caggccgctg gcttcttcga gcagcaggct ttccaggctc
      961 ttggggcagg tcaggatcag ttcatagcgg tcagacatgg ggcatccagt aatcgggtgg
     1021 ccacccggcc accatagtgt gacgcaaggt cgcggtacga cccttcgtcg gaataaacaa
     1081 cgctcgcaga cgagagcggt tcgcaatggc aaaacgcctg ccaacagcgc ctcggctcaa
     1141 caacggcgcg ggccggggca atcggggcag gccaaaagcg gctgcacctt atggctctct
     1201 acccactcag gttacgccct tatgacaaaa cgatcattca cagggtggaa cgcattggat
     1261 agaaatcaac tcaggccggt agcgcaacgc tgctggcagg tggcccgcca cgccggcagc
     1321 gggccccaca ggcagagcat tttctgcccg gtcccaccaa ggggccaacg ggacataaca
     1381 gtcaacagtg agggcaacac ccgatgagaa gacttaagcg tgatccgttg gaacgagctt
     1441 tcttgcgcgg ctatcagcac ggcattcatg gtaaatcccg cgatatgtgt cctttcaccc
     1501 accccaccac ccgtcaagcc tggatcaatg gctggcgcga gggccgcggt gacaactggg
     1561 acggactgac cggcaccgcc ggcatccaca gactcaacga actccacgcc gtcggctgaa
     1621 tcaggatcac cccgacttca ccagaccgtc ccatccggac ggcgggcgag agcccaaggg
     1681 cccctcaggg gcccttttta ttgccagtga gaaacccctt caggtcctcg gcagcgcggc
     1741 actcgcatcc accgcctcgc ggatcagcgc cgggcccttg tagatgaacc cggaatagat
     1801 ctgcaccagg ctggcgccgg cagcgatctt ctcggccgca tgcgcaccct cgatgatgcc
     1861 gcccacggcg atgatcggca ggcggccctt gagctcgccg gccagcaccc gcacggtatg
     1921 ggtgctcttg tcgcgcaccg gcgcgccgga cagaccaccg gcctcgttac cgaacggcag
     1981 gttctccacg ccctcgcggc cgagggtggt gttggtggcg atcactgcgt ccatgccggt
     2041 ctccaccagc gcctgggcca cctgcacggt ttcctcgtcg ctcatgtcag gggcgatctt
     2101 gatcgccagc ggcacgcgct tgccgtgctg cacggccagg tcttcctggc gcaggcgcag
     2161 ggcttcgagc agctgcttga gctggtcacc gaactgcagg ctgcgcaggc ccggggtgtt
     2221 gggcgagctg acgttgacgg tgacgtagct ggcgtgggca tagaccttgt ccaggccgat
     2281 caggtagtcg tccaccgcgc gctcgaccgg agtatcgaag ttcttgccga tgttgatgcc
     2341 cagcacgccc ttgaacttcg ccgcgcgaac ccgggccagc aggtgatcga cgccatggtt
     2401 gttgaagccc atgcgattga tgatcgcggt ggcttccggc aggcggaaca gccgcggctt
     2461 gggattgccc ggctgcgggc gcggggtcac ggtgccgatc tcgacgaagc cgaagccgag
     2521 ctgggcaaag ccgtcgatgg cgtcgccagt cttgtccagg ccggcggcca ggcccacggg
     2581 gttgggaaac tgcagcccca tca
//
LOCUS       DASUYC010000430         3212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1813942, whole genome shotgun sequence.
ACCESSION   DASUYC010000430 DASUYC010000000
VERSION     DASUYC010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1813942"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VF276_07080"
     CDS             <1..528
                     /locus_tag="VF276_07080"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606664.1"
                     /translation="NRLLLVDPRDGSKRLLRQMENNTDQDGIDGLGIDRAGGELLVPD
                     SPNNRLLALSLDGSALRVIARGFQRPTGAAILPGGDILVAAEFGNAIYRVTPAGKSTR
                     IATMYQPDDVVVDAQGMAYVNSLGGAIYKVDPNTGTRTTLLTGLKLPHGLDVDPQGNL
                     VIAEAGRNRIFRLVP"
     gene            956..>3212
                     /locus_tag="VF276_07085"
     CDS             956..>3212
                     /locus_tag="VF276_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606665.1"
                     /translation="MSDENTEAMPAQPGASDGDPQAASGENGDFRIEGQVQLDTAENT
                     HNEMPLRAVAFGKGGQVLGSADVDSKGDFSIPVDLLRPVDVDLYVGPAGDQQPIRESA
                     AYSQSFSAQDWEGEGRVFRLRPNILIPRPIWWPWRPTRICVSGRIRKVHTEHGHTESC
                     PVPYVKVEIFDVDREGCWWPYILRWWDKLIDRPVIRVPDLLKERVVFPRPFPPDPIGP
                     VARFAGLVNPGVIAGFNPQPDPPGVIRGFDPQPDPPGISRALDEVMLNPQPLPPRADM
                     AQAEHAMAATETLSGRVGEMQTLAAHLATRVENLTLTSKIAPWSIFPFCFYSRQLICQ
                     TYTDCQGYFRCCFYWWPLHFRRGRLRFDRRPDIIIRVTQIINGVPTVIYMDPYTSTRW
                     NVTNAYIDLTLDNEEVQCGSDRCPDPPPSGPEVFLTRIGEDEIYKIDQSNGLYPNAAS
                     PDVDLSISNLAYGGDLVVHADFGAALSSGAPKRYYRLSYAKKTNPAVTPPDAAFQPIT
                     SPSSGLSDTRVNKLTFASDTYFLGPQTVGVQPGLYEIRDTVHYHWYNQDRIAEWWSWV
                     DEPGTGTYVLRLEVFDELGNKLTTASGLVNYLDGTHVPTSPATPLPPMIDHCDMVITL
                     DNKGVDVVLHTPATNDCGLIEWTPGLTLNFDVNVHQGHGRLHAWGLYYTKGTDPTPHG
                     LGGGTSNSGAPDPVIQTITVPSNDPMQPMIYNLTGTCAYALKLWGYAHVTDGSHNYLG
                     QHNYLFYNETLQ"
BASE COUNT          580 a         1160 c          998 g          474 t
ORIGIN      
        1 aaccgcctgc tgctggtcga tccgcgcgac ggcagcaagc gcctgctgcg ccagatggag
       61 aacaacacag accaggacgg catcgacggg ctgggcatcg accgggcggg cggcgaactg
      121 ctggtgcccg acagccccaa taaccggctg ctggcgctga gcctggacgg atcggccctg
      181 cgcgtgatcg cgcggggctt ccagcggccc accggcgcgg ccatcctgcc cggcggcgac
      241 atcctggtgg ccgccgagtt cggcaacgcg atctaccgcg tcaccccggc gggcaaaagc
      301 acgcgcatcg ccacgatgta ccagcccgac gatgtggtcg tcgatgccca gggcatggct
      361 tatgtcaact cgctgggcgg cgcgatctac aaggtcgatc ccaataccgg cacgcgcacg
      421 accctgctga ccggcctcaa gctgccgcac ggcctcgacg tggatcccca gggcaacctg
      481 gttatcgccg aggccgggcg caaccgcatc ttccggctcg tcccctgagc aagcacgcgc
      541 ggcacttcgt aaccgttttt gacgccccca caatgtactg gttgacaagc gaatagcaag
      601 cacaagagca gcgcagtacc tcgtagtacc gcaacaccag cgcccaggcg gccctacccc
      661 gcctgctgct cccagatcag agatgtctac cggatagctc tgcccgcgcc cccctgagga
      721 ccggccgcgt gggcacatgc tactccctgc cctgcggcag cgacgatcga gcgtcgcgta
      781 tgttcccggt ataccttgta ataggcgcga tcgcggttga tcgactcaac cgcccgaccc
      841 cgcccggaag attcgtggat tggtggtccc ggcccatggt tggcatggca ccggcaagcc
      901 ggtctgcgcg cacatgacgc agaccctgtt gttgcagcaa aaggagtatt ggaacatgtc
      961 cgacgagaat acggaagcga tgcccgccca gcctggggca agcgacggcg acccccaggc
     1021 ggcgagcggc gagaacggcg attttcgcat cgagggccag gtgcaactgg acaccgccga
     1081 gaacacgcac aacgagatgc cgctgcgcgc ggtggccttc ggcaagggcg ggcaggtgct
     1141 gggcagcgcc gatgtggaca gcaaaggcga cttcagcatc ccggtcgatc tgctgcggcc
     1201 cgtggatgtg gacctgtatg tcggcccggc gggcgaccag cagccgatcc gcgagtccgc
     1261 ggcctacagc cagtcgtttt ccgcccagga ctgggagggc gagggccggg tcttccgcct
     1321 gcgccccaac atcttgatcc cgcggcccat ctggtggccg tggcggccca cgcgcatctg
     1381 cgtctccggg cgcatccgca aggtccacac cgagcacggc cacaccgagt cctgcccggt
     1441 gccctatgtc aaggtcgaga tcttcgacgt ggaccgcgaa ggctgctggt ggccctatat
     1501 cctgcgctgg tgggacaagc tgatcgaccg cccggtcatc cgcgtgcccg acctgctgaa
     1561 agagcgggtg gtgttcccgc ggcccttccc gcccgacccc atcggcccgg tggcgcgctt
     1621 cgccggcctg gtcaaccccg gcgtgatcgc cgggttcaac ccgcagcccg acccgcccgg
     1681 tgtcatccgc ggcttcgacc cgcagcccga cccgcccggc atcagccgcg cgctggacga
     1741 agtgatgctc aacccgcagc cgttgccgcc gcgcgccgat atggcccagg ccgaacacgc
     1801 catggccgcg accgagacgc tgagcgggcg cgtgggcgag atgcagacgc tggccgcgca
     1861 ccttgccacc cgcgtggaga acctgaccct gacctcgaag atcgccccct ggtcgatctt
     1921 cccgttctgc ttctacagcc ggcagttgat ctgccagacc tacaccgact gccagggcta
     1981 cttccgctgc tgcttctact ggtggccgct gcacttccgc cgcgggcgcc tgcggttcga
     2041 ccgccggccc gacatcatca tccgcgtcac ccagatcatc aacggcgtgc ctacggttat
     2101 ctacatggac ccgtacacca gcacgcgctg gaacgtgacc aacgcctaca tcgatctgac
     2161 gctggacaac gaagaagtgc agtgcggcag cgaccgctgc cccgatccgc cgcccagcgg
     2221 gccggaagtg ttcctcaccc gcatcggcga ggacgagatc tacaagatcg accagagcaa
     2281 cggcctctac ccgaacgcgg ccagccccga cgtggacctg agcatcagta acctggccta
     2341 cggcggcgac ctggtagtgc atgccgactt cggcgcggcc ctcagcagcg gcgcgcccaa
     2401 gcgctactac cggctctcct acgcaaagaa gaccaacccg gccgtcacgc cgccggatgc
     2461 ggccttccag ccgatcacca gtccgagcag cggcctgtcc gatacgcgcg tcaacaagct
     2521 caccttcgcc agcgacacct acttcctggg tccgcagacg gtgggcgtgc agccggggct
     2581 atacgagatc cgcgacacgg tgcattacca ctggtacaac caggaccgga tcgccgagtg
     2641 gtggagctgg gtggacgagc cgggcaccgg cacctatgtg ctgcgcctgg aggtgttcga
     2701 cgagttgggc aacaagctga ccaccgcctc cggcctggtc aactacctgg acggcacgca
     2761 tgttccgacc agcccggcga cgccgctgcc gcccatgatc gaccattgcg acatggtgat
     2821 caccctggac aacaagggcg tggatgtcgt gctgcacacg cccgccacca acgactgcgg
     2881 cctcatcgag tggacgccgg ggctgacgct caacttcgac gtgaacgtcc accaggggca
     2941 cgggcggttg cacgcctggg gcctgtacta caccaagggc accgacccca cgccgcacgg
     3001 cctgggcggc ggcacgtcga acagcggggc gcccgacccg gtgatccaga cgatcaccgt
     3061 gccgtccaac gacccgatgc agccgatgat ctacaacctg accggcacct gcgcctacgc
     3121 gctcaagctc tggggttacg cgcacgtgac cgacggcagc cacaactacc tgggccagca
     3181 caactacctg ttctacaacg agacgctcca ga
//
LOCUS       DASUYC010000431         2032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1411421, whole genome shotgun sequence.
ACCESSION   DASUYC010000431 DASUYC010000000
VERSION     DASUYC010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411421"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1708
                     /locus_tag="VF276_07090"
     CDS             <1..1708
                     /locus_tag="VF276_07090"
                     /inference="COORDINATES: protein motif:HMM:NF024295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 44 protein"
                     /protein_id="HEX6606666.1"
                     /translation="QAARTPTPAVAVAERPPTATPAPEATATVDDPTRYAVAVSLDTS
                     VHHPISPLIYGVADAGSDHLDQLTWLGATLVRWGGNARTRHNWEINASNTGIDGGYQN
                     VSQGDKVPGSASLQFLARNAQLGAHSLLTIPTIGWVARDGTSASVDVPEHGGPPVAPG
                     SSAAFTAISGGAWMAPYDPAANRKRTSLPSLARKGAPFSYPPDLTDGKVYQDEWVAYL
                     RGARPADTAPLIYAMDNEPELWSDDTHVDIHPVRTGYDEQAATFLDYARAVKDADPDA
                     LVAGPESWGVTGYLYSALDAGADNFKTAADRAAHGNLPWLAWFLQTVHDSDQQAGRRS
                     LDVLSLHYYPNGGEYGGGNDPATQDRRMQAPRALWDGLFVEPSWVARTDWSNLGLLPR
                     LRKLIAQYYPGTRLAVSEWNYGGETDISGAIATADALGIYGREDVYYAAYWQAPPVNS
                     PGGWAFRLFRNYDGNGAAFGNESVQTSSLNPLLTAYGALSTDGTRLTVLLINKDRART
                     ADAQLDLAGFAPAPAATEYRYGPAHLDGIVSAPLTVTTGGPPVVAVPPTSMSLVVLDR
                     NR"
     gene            1720..>2032
                     /locus_tag="VF276_07095"
     CDS             1720..>2032
                     /locus_tag="VF276_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606667.1"
                     /translation="MAVTFAPTEHAPMSRRNRVFPELTAPRSRFGLAFRQWAAALATV
                     AVFAGLAALLAVVGDRPETVISLGLVLLSVMAVLRWPIVGTYLAVLSTIILDTFPSPY
                     VQ"
BASE COUNT          308 a          769 c          671 g          284 t
ORIGIN      
        1 gcaagcggcc cgcaccccca cgccggcggt cgcggtcgcc gagcgcccgc ccacggccac
       61 gcccgcgccc gaagcgaccg cgaccgtgga cgacccgacg cgctatgccg tggccgtgag
      121 ccttgatacg agcgtccacc accccatcag cccgctcatc tacggcgtgg ccgacgcggg
      181 gtcggaccat ctcgatcaac tgacctggct gggcgccacg ctcgtgcgct ggggcggcaa
      241 cgcccgtacc cgccacaact gggagatcaa cgcctcgaat accggcatcg acggcggcta
      301 ccagaacgtc agccagggcg acaaggtgcc tggcagcgcc tcgttgcagt tcctggcgcg
      361 caacgcccaa ctgggcgcgc acagcctgtt gacgatcccg acgattggct gggtggcccg
      421 cgacggcacc agcgcctcgg tcgatgtgcc ggagcacggc ggcccgccgg ttgcgcccgg
      481 cagcagcgcg gccttcacgg cgatcagcgg cggcgcctgg atggccccct acgacccggc
      541 ggccaaccgc aagcgcacca gcctgccgtc gctggcgcgc aagggcgcgc cgttcagcta
      601 cccgcccgac ctgaccgacg gcaaggtcta ccaggatgag tgggtggcct atctgcgcgg
      661 cgcgcgcccg gccgatacgg ccccgctcat ctacgccatg gacaacgagc cggaactctg
      721 gtcggacgat acgcacgtcg atattcaccc cgtgcgcacc ggctacgacg aacaggcggc
      781 aaccttcctg gactacgctc gcgcggtcaa ggacgccgac cccgacgcgc tggtggccgg
      841 ccccgaaagc tggggcgtga ccggctatct ctactcggcg ctcgacgcgg gcgcggacaa
      901 tttcaagacc gccgccgacc gcgcggccca cggcaatctg ccctggctgg cctggttcct
      961 ccagaccgtc cacgacagcg accagcaggc gggccggcgc tcgctcgacg tgctgagcct
     1021 ccattattac ccgaacggcg gggagtacgg cggcggcaac gacccggcca cccaggaccg
     1081 gcgcatgcag gcgccccgcg ccctgtggga cgggctgttc gtggagccaa gctgggtggc
     1141 gcgcaccgac tggtcgaacc tggggctact gccccggctg cgcaagctga tcgcgcagta
     1201 ttatcccggc acccgcctgg ccgtcagcga gtggaactac ggtggggaga ccgacatcag
     1261 cggcgccatc gccaccgccg atgcgctggg catctatggc cgggaagatg tgtattacgc
     1321 ggcctactgg caggcgccgc cggtgaacag ccccggcggc tgggccttcc ggctgttccg
     1381 caactacgac ggcaacggcg cggccttcgg taacgagtcg gtccagacca gcagcctcaa
     1441 tccgctgctc accgcctacg gcgcgctcag cactgacggc acgcgcctga cggtgctgct
     1501 gatcaacaag gaccgcgccc gcaccgccga cgcgcaactc gacctggccg gcttcgcccc
     1561 ggccccggcg gccacggagt accgctacgg cccggcccac ctcgacggca tcgtctccgc
     1621 gccgctgacc gtgaccaccg gcggcccgcc ggtggtcgcc gtgccgccca ccagcatgag
     1681 cctggtcgtt ctcgaccgca atcgctagga aacatcacga tggctgtgac cttcgcccca
     1741 accgaacacg cgcctatgtc ccggcgcaac cgggtcttcc ccgagttgac cgcgccccgg
     1801 tcgcgcttcg gcctggcttt ccgccaatgg gcggcggcgc tggcgacggt cgccgtgttc
     1861 gccgggctgg cggcgctgct cgcagtcgtg ggcgaccggc cggagacggt gatctcgctc
     1921 ggcctggtgc tgctgagcgt gatggccgtg ttgcgctggc cgattgtggg tacgtacctg
     1981 gccgtgctgt ccacgatcat cctcgatacc ttcccgtcgc cctatgtgca ga
//
LOCUS       DASUYC010000432         4030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1638697, whole genome shotgun sequence.
ACCESSION   DASUYC010000432 DASUYC010000000
VERSION     DASUYC010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1638697"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            358..2238
                     /locus_tag="VF276_07100"
     CDS             358..2238
                     /locus_tag="VF276_07100"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HEX6606668.1"
                     /translation="MLQDADIQTPAALPAPEGAADESADGRPTGAPPAAPETPDSNLV
                     LNPAQQETLTQERRLLGALGAAITPLEPASGDLDALRQAGLDLDELFLLVIAGEFNAG
                     KSAFINALLGERFLTEGVTPTTAVVTLLRYGDQPTDEPVGEDLAERAYPAPFLRDITV
                     VDTPGTNAIIRRHEQLTRHFVPRADLVLFVTSADRPFTESERAFLETIRDWGKKIVIV
                     LNKIDLMASATDLQHVIDFIARNAQALLGVTPDIFPVSARLALQGKRLRAGEDRQQRA
                     QGETLLRASRFDRLESYIFATLDEEGRLRLKFLTPLGIAERVLEKYRKVATERLAVLA
                     DDFKTNENIDAQLKVYQEDMRTQFEHRLAEIENIIYEMRERGDAFFEEMVRITHIRQL
                     MNAELVRGAFEREVVADSAQRVDAAVQGLIDWMVDHDLRMWQSIQEYLDRRKAVRGRE
                     DQMIGQVGRQFEYNRTQLLQSVGSRATKVIQGYDREREARELAEEMRAAVNQTIGAAG
                     LAGVGALAAAIFTAALFDATAVLISTLAAGFALFVLPLKKRQAKEQFHYKVQELRENL
                     ARGMTEQFDAELARSVERIYDAIAPYTRFVKAEHTKVSTAGEELARLDGEFARLRKRI
                     EG"
     gene            2250..3044
                     /locus_tag="VF276_07105"
     CDS             2250..3044
                     /locus_tag="VF276_07105"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX6606669.1"
                     /translation="MSIRVAAVQAPTPTGRDGARALAEIIGGAVIAGAELIALALTPP
                     PRPPAAADPTPRALRGLAAGHNVWLVAGPYVERDTDGRAYLAAGLWDPEGTLIASARQ
                     THQPPGTGWTAADTLAPLPLTGRDGARWPLGLILGEDIRYPEVGRILALQGATVLIHL
                     GVMPAPYNPWEQERRLWREVQANQTFGLESYPLGPGGGGYARVLAPCEMTPGGSGILA
                     ATHSLRDPAAAVADLNAGAREAVIAAYPIASQFNRDMYHKLPGAYD"
     gene            3037..>4030
                     /locus_tag="VF276_07110"
     CDS             3037..>4030
                     /locus_tag="VF276_07110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX6606670.1"
                     /translation="MIRGPRRELWLLGGILLLAALARYTGLTQGFPRDFHWDERIYFH
                     EALYGLANGLRRESTVSANLPYLLMPWLLAQWLIATLAGQMHGFGDLVRSYIVDPAPF
                     LAVARAAWAATGVISVAAVYRLGRRMAGPAVGLVAAFFLAAAFLHVAESHFIKNDVPA
                     GLMLLLALDACFNIQERGARRDYFWAGFWLGLASATKYYAAGVAPVILLAHFLRREPA
                     GAAPSRLRGLALLGIALGGALLAAVASTPVAVIDPRNLWDSLSYEFGAHFGSIPTDGV
                     PQWMFYLTEHLGRGMGAMLLGLALIGIGIVLTRRTPKGVLLAAVPVLFFIALNLR"
BASE COUNT          616 a         1415 c         1390 g          609 t
ORIGIN      
        1 gatgggcggg gtgcgcaggc gcgtccaggg gggcgggcac ggctccggcc aggatctcgt
       61 cgtcggcgtg ttgggtcggc aatgcactac ctcgctacag agactaacgt gtcgcgaccg
      121 gcacggttgc accttacgtg gaacttacca tacccgataa tggaccgatc ggcaagggcc
      181 atgccaggcc ccggttcggc ccgacgggcc gctagcatgc cgctctccgc cgtgttacaa
      241 taggcgtaga agacgtatgg tatacatcgt gcttgattaa gcggtgtaga atcatgccgc
      301 ccggcgcccg gatggccgga cccgcctcgt gaggcgagcg aacacaggag taacagcgtg
      361 ctgcaagacg ccgacataca aacacctgcc gccctgccgg cccctgaagg ggcagcggac
      421 gagtcggcgg acggccgccc gaccggcgcg ccgcccgccg cgccggaaac accggactcc
      481 aacctggtcc tgaaccccgc gcaacaggaa accctgaccc aggagcggcg cctgctcggc
      541 gcgctcggcg ccgccatcac cccgctggag ccggcctccg gcgacctgga cgccttgcgc
      601 caggccgggc tcgatctcga cgagttgttc ttgctggtca tcgccgggga gttcaacgcg
      661 ggcaagtcgg cctttatcaa cgcgctactg ggcgagcgct tcctgaccga gggggtgacg
      721 ccgacgacgg ccgtcgtgac cctgttgcgc tacggcgacc agccgaccga cgagccggtg
      781 ggcgaggatc tggccgagcg cgcctacccc gcgccgttcc tgcgcgacat caccgtggtc
      841 gatacaccgg gcaccaacgc cattatccgc cgccacgagc aactgacccg ccatttcgtg
      901 ccccgcgccg acctggtgct cttcgtgacc tcggcggacc ggcccttcac cgagagcgaa
      961 cgcgccttcc tggagacgat ccgggactgg ggcaagaaga tcgtcatcgt gctcaacaag
     1021 atcgacctga tggcgagcgc aaccgacctg cagcacgtga tcgactttat cgcgcgcaac
     1081 gcccaggcgc tgctcggcgt cacgcccgat atcttcccgg tgtcggcccg gctggcgctg
     1141 caaggcaagc gcctgcgtgc cggcgaggac cggcagcagc gcgcccaggg cgaaacgctg
     1201 ctgcgcgcca gccgcttcga ccggctggag tcctatatct ttgcgacgct ggatgaagag
     1261 ggccgtctgc gcctgaagtt cctgaccccg cttggcatcg ccgagcgcgt gctggagaag
     1321 taccgcaaag tcgccaccga gcgactggcg gtgctggccg acgatttcaa gaccaacgag
     1381 aacatcgacg cgcaactgaa ggtctaccag gaggacatgc gcacgcagtt cgagcatcgc
     1441 ctggccgaga tcgagaacat catctacgag atgcgcgagc gcggcgacgc cttcttcgag
     1501 gaaatggtgc gcatcacgca tatccgccag ttgatgaacg ccgagctggt gcgcggcgcc
     1561 ttcgagcgcg aggtggtggc cgacagcgct cagcgggtcg atgccgccgt gcagggcctg
     1621 atcgactgga tggtggacca cgacctgcgc atgtggcagt cgatccagga gtacctggac
     1681 cggcggaagg cggtgcgcgg gcgcgaggac cagatgatcg ggcaggtggg gcggcaattc
     1741 gagtacaacc gcacccagtt gttgcagtcg gtgggcagcc gggccaccaa ggtgatccag
     1801 ggctacgacc gcgagcgcga ggcgcgggaa ctggccgagg agatgcgcgc cgcggtgaac
     1861 cagaccatcg gcgcggcggg gctggccggg gtcggcgcgc tggccgccgc catcttcacc
     1921 gcggccctgt tcgacgccac tgccgtgctg atctccacgc tggccgccgg gttcgcgctg
     1981 ttcgtgctgc cgctgaagaa gcgccaggcc aaggagcagt tccactacaa ggtgcaggag
     2041 ttgcgcgaga acctggcccg cgggatgacc gaacaattcg acgccgagct cgctcggtcg
     2101 gtcgagcgca tctacgacgc catcgcgcct tatacccgct tcgtcaaggc cgagcacacc
     2161 aaggtcagca ccgccggtga ggaactagcg cgcctggatg gcgagttcgc ccggctgcgc
     2221 aaacgcatcg aaggctgaat cgcccaccca tgagcatccg cgtcgccgcc gtgcaggcgc
     2281 ccacgcccac cgggcgcgac ggcgcccgcg cgctggccga gatcatcggc ggggccgtca
     2341 tcgcgggcgc cgagttgatc gctctggcgc tcacgccccc gccccgcccg cccgccgccg
     2401 ccgacccgac cccgcgcgcc ctgcgcggcc tggccgcggg gcacaacgtc tggctggtgg
     2461 ccggccccta tgtcgagcgc gacacggatg gccgggccta cctggccgcg ggtctctggg
     2521 acccggaggg cacgctgatc gccagcgcgc gccagacgca ccagccgccg ggcactggct
     2581 ggaccgccgc cgatacgctg gcccccctgc ctctgaccgg ccgtgacggc gcacgctggc
     2641 cgctgggcct catcctgggc gaggatatac gctacccgga ggtggggcgc atcctggcct
     2701 tgcagggcgc cacggtgctg atccatctgg gagtcatgcc ggccccgtat aatccgtggg
     2761 agcaagagcg gcggctctgg cgcgaggtgc aggccaacca gaccttcggc ctggaaagct
     2821 atccgctggg gccggggggc ggcggctacg cgcgcgtgct ggccccctgc gagatgacgc
     2881 ccggcggcag tggcatcctg gcggcgaccc actccctgcg cgacccggcg gcggctgtcg
     2941 cggacctgaa cgccggggcg cgggaagccg tcatcgccgc gtacccgata gccagccagt
     3001 tcaacaggga tatgtatcac aaactaccgg gcgcgtatga ttagaggccc tcgccgcgaa
     3061 ctctggctac tcggcggcat cttgctgctg gccgcgctgg cccgctatac cggcctgacc
     3121 cagggcttcc cgcgcgactt ccactgggac gagcgcatct acttccacga ggcgctctac
     3181 gggctggcga acggcctgcg ccgcgagtcc accgtctcgg ccaacctgcc ctatctgctg
     3241 atgccctggc tgctggcaca gtggctcatc gccaccctcg ccgggcagat gcacggcttc
     3301 ggcgatctcg tccgcagcta tatcgtggac cccgcgccgt tcctggccgt ggcccgcgcc
     3361 gcctgggccg ccacaggggt gatcagcgtg gccgcggtct atcgcctcgg gcggcgcatg
     3421 gccggcccgg cggtgggcct ggtggccgcc ttcttcctgg ccgcggcctt cctgcatgtg
     3481 gccgagagcc atttcatcaa gaacgacgtg cccgccgggc tgatgctgct gctggcgctc
     3541 gacgcctgct tcaacatcca ggagcgcggc gcgcggcgcg actatttctg ggccgggttc
     3601 tggctgggcc tggcgagcgc caccaagtat tacgccgccg gggtcgcgcc ggtcatcctg
     3661 ctggcgcact tcctgcggcg ggagcccgca ggggcggctc cctcccggct gcggggcctg
     3721 gcgctgctcg gtatcgcgct gggcggcgcg ctgctggccg cggtcgcctc gacgccggtg
     3781 gccgtgatcg acccgcgcaa cctctgggac agcctgtcct atgaattcgg cgcccacttc
     3841 ggatccatcc cgacggacgg cgtgccgcag tggatgttct acctgaccga gcacctgggc
     3901 cggggcatgg gcgcgatgct gctgggcctg gcgctgatcg gcatcgggat cgtgcttacg
     3961 cggcgcacgc ccaagggcgt gctgctggcg gcggtgccgg tgctgttctt cattgcgctg
     4021 aacctgcggc
//
LOCUS       DASUYC010000433         4934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_9888,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000433 DASUYC010000000
VERSION     DASUYC010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4934
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9888"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1550
                     /locus_tag="VF276_07115"
     CDS             <1..1550
                     /locus_tag="VF276_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX6606671.1"
                     /translation="ITTAIPYVNARPHIGFALEIIQTDTFARYHRQRGEDVYFLTGSD
                     ENSLKNVQAAEAEGISTRELVDRNATAFEGLRPLLNLSFDQFIRTSADPRHAAGAQKL
                     WEACAANGDIYQKTYRGLYCVGCEQFYTPAELTPEGLCPEHLTRPDVVEEENYFFRLS
                     NYGPQLLQLIESGELRIIPESRRNEVLSFIRMGLEDFSISRSVARARNWGVPVPGDPG
                     QVMYVWFDALTNYITALDYAHDGPLYRRYWAESPQRVNTIGKGVIRFHAVYWPAMLLS
                     AGVRPPSTVFVHGYISVAGQKMSKSLGNVVDVYDLVEKYGAEAVRYFLLREVPATADA
                     DFTYEKFEARYNSDLANDLGNLLNRAVSMIHRYRGGTVPAPGDPDALDTELRAVAEGI
                     PARVAAALADYDPQEALDAIWELVKRANQYVEQNQPWTLAKTAKGGDAGAAGRLDSVL
                     YNLAESLRLLAIHLAPFLPATAAGIADQLGQPGALAAPYAEAVRWGGTAPGSAVAEAH
                     PLFPRLQ"
     gene            1684..2760
                     /gene="cax"
                     /locus_tag="VF276_07120"
     CDS             1684..2760
                     /gene="cax"
                     /locus_tag="VF276_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HEX6606672.1"
                     /translation="MSPAALLNTFDRKEQITLAIGIVGTIVTAILQAMQVNSLIVFVA
                     AAITMAALAGAVGDSTDQLGSRLGPGATGVLQSALGNLPELFIAIFALQAGLVTVVQA
                     ALVGSILGNSLLVLGLAFLVGGLKHGTQRFGQPQSRMIAMLIMLAVAALAIPTIATRP
                     GQPDAPHAEELSVICAVVLLIVFAASIPFSLGGGPGASSQGVSTEKHWPMPLTIAILA
                     GAGIAAAFVSDWFVAALKPTIEAAHISEGFAGLVVVAIAGNAVENVVGVQMAARNEMD
                     LAISVILNSSLQVAVALTPALVLISLFLAGHLLLVLPVLLLAALGLAAVLGALVVYDG
                     ESTWLEGLALIGLYVIIAASFWWG"
     gene            3174..>4934
                     /locus_tag="VF276_07125"
     CDS             3174..>4934
                     /locus_tag="VF276_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606673.1"
                     /translation="MLEPDAPRQFAPIEDLDPEPPGRQAHRRELILALAILIVVVGLA
                     GGNWWDQQRKLTHYQAGGRAAAARDWDRAQGEYAAAGDYADAPRRATDAARQVAERDA
                     QYAVATDAARHDNWLLALLAIRKVAAIQPQYRDTPALQRQAETQAYTAALSGTIALRP
                     QATPPGLYSYTADGWRFLAGSDGGSRVRGTCADGGMLFDTPRTGRARGPGPGITSSLI
                     LPAGAGRDLRRAAPDGTPDPAAPVRYNPATYQYTCTPGGLWGILQRQDGGIALNDGQG
                     ADYPFWLMYQTAGQRGPVPLRLPDQTWPIRVLAVPPRGEQILITGLTDISPAMLRPGP
                     PPAMARTRFYLADADGSNPRLVDDLAGWPGSTEISPDGRFVLLTMYRPLTGNRGLATT
                     LLLDLTGQMPTRPLAQMPLDLDHQRAAGDAAFIREGPRRGQVLTVGDDLRDTQLRIYD
                     PARRDLDPLVQVPAPPDLSEITRLQAAPDGALLITVADTSYVTGGRTGLLAYLDPQAR
                     LTLIHPAPGADLYLSQAWLQPGRLVYELQDSGVDREAHYAVYSLALDQLGDADPVPVQ
                     TYSVTIDTQEWNELTTTLTSW"
BASE COUNT          758 a         1829 c         1608 g          739 t
ORIGIN      
        1 tgatcaccac ggccatcccc tacgtcaacg cccggccgca catcggcttc gccctggaga
       61 tcatccagac cgacaccttt gcccgctacc accggcagcg cggcgaggat gtctactttc
      121 tgaccggcag cgacgagaac agcctgaaaa acgtgcaggc cgccgaggcc gagggcatct
      181 ccacgcgcga actggtggac cgcaacgcca cggcgttcga gggcctgcgc ccgctgctca
      241 acctgtcgtt cgaccagttc atccgcacca gcgccgaccc gcgccacgcc gcgggcgcgc
      301 aaaagctctg ggaagcctgc gccgccaacg gcgacatcta tcagaagacc tatcgcggcc
      361 tctactgtgt gggctgcgag cagttctaca cgcccgccga gttgacgccg gaagggctgt
      421 gccccgaaca cctgacccgg cccgacgtgg tggaggagga gaactacttc ttccgcctgt
      481 cgaactacgg gccgcagttg ctgcaactga tcgagtcggg cgagttgcgg atcattccgg
      541 agagccggcg caacgaggtg ctcagcttca tccgcatggg gctggaagac ttcagcatct
      601 cccgctcggt ggcgcgcgcc cgcaactggg gcgtgccggt gcccggcgac cccggccagg
      661 tgatgtacgt ctggttcgac gcgctgacca actatatcac cgcgctcgac tacgcccacg
      721 acggcccgct ctaccggcgc tactgggctg agagcccgca gcgggtcaac accatcggca
      781 agggcgtgat ccgcttccac gccgtctact ggccggccat gctgctgtcg gcgggagtgc
      841 gcccgcccag taccgtcttc gtccacggct acatcagcgt ggccgggcag aagatgagca
      901 agtcgctcgg caacgtggtc gacgtgtacg acctggtgga gaagtacggc gcggaggccg
      961 tgcgctactt cctgctgcgc gaggtgcccg ccaccgccga cgccgacttt acctatgaga
     1021 agttcgaggc gcgctacaac agcgacctgg cgaacgacct gggcaatctg ctcaaccggg
     1081 cggtcagcat gatccaccgc taccgcggcg gcaccgtgcc cgcgcccggc gaccccgacg
     1141 cgctggacac ggagctgcgc gccgtggccg agggcatccc cgcccgcgtg gcggcggccc
     1201 tggcggacta cgacccgcag gaagccctgg acgccatctg ggaactcgtt aagcgggcca
     1261 accagtacgt cgagcagaac cagccctgga cgctggcgaa aacggccaag ggcggcgatg
     1321 ccggcgcggc gggccgcctc gacagcgtgc tctacaacct ggccgagtcg ctgcgcctgc
     1381 tggcgatcca tctcgcgccg ttcctgcccg cgaccgccgc cggcatcgcg gaccagttgg
     1441 ggcagccggg cgcgctcgcc gcgccctatg ccgaggctgt gcgctggggc ggcaccgcgc
     1501 ccggcagcgc cgtcgccgaa gcacacccgc tcttcccgcg cctgcaatag cgcggggcag
     1561 caagaaacgc gggccggagc gcatctccgg cccgcgtttt gcgttgtaag cttcgcttgt
     1621 gccgcaaacg cggccacaag gcttaccggc ccattctcaa tatccccaac aggagtgcag
     1681 tgtatgagtc ccgcggccct cttgaacacc tttgaccgca aagagcagat caccctggct
     1741 atcggcatcg tgggcacgat cgtgacggcg attttgcagg ccatgcaggt caattcgctg
     1801 atcgtcttcg tcgcggcggc aatcaccatg gctgcgctgg ccggggccgt gggcgacagc
     1861 accgatcagt tgggcagccg cctggggccg ggcgccaccg gcgtgctgca gtcggcgctt
     1921 ggcaacctgc ccgaactgtt catcgccatc tttgccttgc aggccgggct ggtcacggtg
     1981 gtgcaggcgg cactggtcgg ctcgatcctg ggcaacagcc tgctggtgct ggggctggcc
     2041 ttcctggtcg gcggcctcaa gcacggcacg caacgcttcg gccaaccgca gagccgcatg
     2101 atcgccatgc tgatcatgct cgccgtggcc gccctggcga tccccacgat tgccacgcgc
     2161 cccggccagc ccgatgcgcc gcacgccgag gaactgagcg tcatctgcgc ggtcgtgctg
     2221 ctgatcgtct ttgccgccag cattcccttc tcgttgggtg ggggaccggg cgccagttcg
     2281 caaggcgtgt ccacggagaa gcactggccc atgccgctga cgatcgccat cctggccggg
     2341 gccggtatcg ccgctgcctt tgtgtccgac tggtttgtcg cggccctcaa gccgaccatc
     2401 gaggccgccc acatctcgga gggcttcgcg gggctggtcg tggtcgccat cgccgggaac
     2461 gccgtcgaaa acgtggtcgg cgtgcagatg gccgcgcgca acgagatgga cctggcgatc
     2521 agcgtcatcc tcaacagcag cttgcaggtc gcggtcgcgc tcacccccgc gctggtcctc
     2581 atcagcctgt tcctggccgg gcacctgttg ctggtgctgc cggtgctgtt gctggccgcc
     2641 ctgggcctgg ccgccgtgct gggcgccctg gtcgtctacg acggcgagtc gacctggctg
     2701 gagggcctgg ccctgatcgg cctctatgtg atcatcgccg ccagcttctg gtggggctag
     2761 ccgccgggca gcaggccggt tggtacccca cgcctacacc gcggcggcag gccggttgca
     2821 agcggatctc tacaccgggc agcaggccgg tttgaaaccg gcccctacac cgcgatttta
     2881 cgatggcggg ctaatccctg cccaccgccg atatgtgcag ctgtttcaaa cgggcacgcc
     2941 gccccatccg cttattccgc ggtgtagggg ccggtttcaa accggcccgg cgggcgcatc
     3001 atccgatcat ttcgcggtgt agaggccggt ttcaaaccgg cccggcgggc gcatcatccg
     3061 gtcatttcgc ggtgtagggg ccggtttcaa accggcccgc cgctgccaca tccggtataa
     3121 tacaagcagg atcgcaaaca tcgccgccgc cgaccggcgc gggaggagcc gccatgctgg
     3181 agccggatgc gccccgccaa tttgccccca tcgaggacct ggacccggag ccgccgggcc
     3241 gccaggccca ccgccgcgag ctcatcctcg cgctggcgat cctgatcgtg gtcgtggggc
     3301 tggccggggg caactggtgg gaccagcagc gcaaactcac ccactaccag gcgggcgggc
     3361 gcgccgccgc tgcgcgcgat tgggaccgcg cccagggcga atacgccgcc gcgggcgact
     3421 acgccgatgc cccgcgccgc gccaccgacg ccgcgcgcca ggtagccgag cgcgacgcgc
     3481 aatacgccgt cgccacggac gccgcgcggc acgacaactg gctcctggcc ttgctggcga
     3541 tccgcaaggt cgcggccatt cagccgcagt accgcgacac ccccgccctg caacggcaag
     3601 ccgagaccca ggcctacacg gcggccctga gcggcaccat cgccctgcgt ccgcaggcca
     3661 cgccgcccgg tctctacagc tatacggcgg acggctggcg gttcctggcc ggcagcgacg
     3721 gcggcagccg ggtgcgcggc acctgcgccg acggcgggat gctgttcgat acgccgcgta
     3781 cgggccgcgc gcggggcccc ggccccggca tcaccagcag cctcatcctg cccgcgggcg
     3841 ccgggcgcga cttgcgccgc gcggcccccg acggcacacc cgatcccgcg gcgccggtgc
     3901 gttacaaccc ggccacctac caatacacct gcacgccggg cggcctctgg ggcatcttgc
     3961 agcggcagga cggcggcatc gccctgaacg acggccaggg cgccgattat cccttctggc
     4021 tcatgtatca gacggcgggc cagcgcggcc cggtccccct gcgcctgccc gaccagacct
     4081 ggcccatccg cgtcctggcc gtgcccccgc gcggcgagca gattcttatc accgggctga
     4141 cggacatcag tcccgccatg ttgcgaccgg ggccgccgcc cgccatggcc cgcacccgct
     4201 tctacctggc cgatgccgat gggagcaatc cgcgcctggt ggatgacctc gccggctggc
     4261 cgggatcgac cgagatcagc ccggacgggc gcttcgtgct gctgaccatg tatcgcccgc
     4321 tcaccggcaa ccgcgggtta gccaccacgc tgctgctgga cctgaccggc cagatgccta
     4381 cgcgcccgct ggcccagatg ccgctcgacc tcgaccacca gcgcgccgcc ggcgacgccg
     4441 cgtttatccg cgagggaccg cgccggggcc aggtgctcac cgtgggcgac gacctccgcg
     4501 acacgcaact ccgcatctac gatccggccc ggcgggatct cgacccgctc gtccaggtgc
     4561 ccgccccgcc cgacctgagc gagatcaccc gcctgcaagc ggcacccgac ggcgcgctgc
     4621 tcatcacggt ggccgataca tcctatgtca ccggcgggcg gaccgggctg ctggcctatc
     4681 tcgacccgca ggcccgcctg accctcatcc atccggcccc cggcgccgac ctctatctga
     4741 gccaggcctg gctgcagccg ggccggcttg tctacgaact ccaggactcc ggcgtggaca
     4801 gggaggccca ctacgccgtg tacagcctcg cgctggacca attaggcgac gccgatccgg
     4861 tgcctgtcca gacctacagc gtgaccatcg atacgcaaga atggaacgaa ctgacgacga
     4921 cgctcacctc ctgg
//
LOCUS       DASUYC010000434         2650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1752289, whole genome shotgun sequence.
ACCESSION   DASUYC010000434 DASUYC010000000
VERSION     DASUYC010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1752289"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1486
                     /locus_tag="VF276_07130"
     CDS             <1..1486
                     /locus_tag="VF276_07130"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEX6606674.1"
                     /translation="VALGVLALLGMLGPFSIVFSWLHGLVPGFDKFRAAGRLLLLLGL
                     ANAGLAALGLDALLRLLPAAARVPGRAPAPEVPAEPDVVLVGMAPPEPAAEWLGAPLD
                     EDARPGPAQQRRLQRWVLMIAALALFVILVLLPISTSMFLSGFSIERGQIVLNDLYSA
                     ALWVALMAGLLLAAVRGRIAGRAAALLLAALLVIDLFSPNSHFNPTSTNILSGYNQAN
                     AIDYIQENTGPPNFYRMDSDTDVSAVWQPMLGTLVGVPDTGGVYNPLELRRYAAYWDA
                     AKADNRGSVLYNLLSVKYTAVLSAGLVANPKFNFVVNGNQGVRVVENKLVYPRFFLVH
                     DAAVEPDDARALGKLANGTVLGWHTIYLPDGAGRQSATPSTLEGGAPGERAWITEYTP
                     NRIRAEVDATEAGWAVFSEVWYPGWEATVDGRPVPIERADTLFRAVAVTPGHHTIELH
                     LQAPTLRLGLLISGLTLLLTVLGGAVAFLWYRRTRRPLNRSALL"
     gene            complement(1516..1968)
                     /locus_tag="VF276_07135"
     CDS             complement(1516..1968)
                     /locus_tag="VF276_07135"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX6606675.1"
                     /translation="MSAEQNKAVFRRVIEEGFNGGNLDALNDCFPPAYAEHQFDLPAT
                     LEEFKGSIRFLRETFAPFSLTIEDMVADGDKVWARMTGRGTDGKGLMGRPPTGRSFAI
                     TVIDVCRFENGKIVEHWGVPDRFHQMVQLGLLPQPGEQPAQAVAAGRV"
     gene            complement(2108..>2650)
                     /locus_tag="VF276_07140"
     CDS             complement(2108..>2650)
                     /locus_tag="VF276_07140"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX6606676.1"
                     /translation="AESLRRQHAAGNPAGIAEGLGGLAALALAQGRLDRAARLFGAAE
                     ALRAAHPAPIWPAERFEIDRHTEDLRARLPAPLWRQLRHAGQALPVEQAVAYALAGEE
                     PAGPQQPPARFGGLTDREREVAALIAQGATNRAIAEALVISERTVERHVANIFAKLDM
                     GSRSQIAVFAVETGLTHPGA"
BASE COUNT          370 a          922 c          918 g          440 t
ORIGIN      
        1 ggtggccctg ggggtgctgg ccctgctggg catgctcggc ccgttcagca tcgtcttttc
       61 ctggctgcac ggcctggtac ccggcttcga caagttccgt gccgcggggc ggctgttgct
      121 gctgctaggc ctggcaaacg ccgggctggc ggcgctgggc ctcgatgcgc tgttgcggct
      181 gctgcccgcc gcggcgcggg tgccgggccg tgcgcccgcc ccggaggtgc ccgccgagcc
      241 cgatgtcgtc ctggtcggta tggcgccgcc ggaacccgcc gccgagtggc tgggcgcgcc
      301 gctggacgaa gatgcccgcc ccggcccggc ccagcagcgc cggttgcagc gctgggtgct
      361 catgatcgcg gcgctggcgc tgttcgtgat cctggtgctg ctgcccatct ccacgtcgat
      421 gttcctgagc ggcttttcca tcgagcgcgg gcagatcgtg ctgaacgacc tctattcggc
      481 ggccctgtgg gtggcgctca tggccgggct gctgctggcc gccgtgcgcg ggcgcatcgc
      541 ggggcgcgcg gcagccctgc tgctggccgc gctgctggtg atcgacctct tctcgcccaa
      601 ctcgcacttc aacccgacca gcaccaatat cctgagcggc tataaccagg ccaacgccat
      661 cgactatatc caggagaaca ccggcccacc caacttctat cgcatggaca gcgacaccga
      721 cgtgtccgcc gtctggcagc cgatgctcgg cacgctggtg ggcgtgcccg acacgggcgg
      781 ggtctacaac ccgctggaac tgcgccgcta tgccgcctac tgggacgccg cgaaggccga
      841 taaccgcggc agcgtgctct ataatctgtt gagcgtgaag tacaccgccg tgctgtccgc
      901 ggggctggtc gccaatccca agttcaactt cgtggtcaac ggcaaccagg gcgtgcgcgt
      961 ggtcgaaaac aagctggtgt acccgcgctt cttcctggtc cacgacgcgg cggtcgaacc
     1021 cgacgacgcc agggcgctcg gcaagctggc gaacggcacc gtcctgggct ggcacacgat
     1081 ctacctgccg gacggcgcgg gccgccagag cgccacgccc agcacgctgg aaggcggcgc
     1141 ccccggcgaa cgcgcctgga tcaccgagta cacgcccaac cgcatccgcg ccgaggtgga
     1201 cgcgacggag gccggttggg ccgtcttcag cgaggtctgg tatcccggct gggaggcgac
     1261 ggtcgacggc cggcccgtgc ccatcgagcg cgccgacacg ctgttccgcg ccgtggccgt
     1321 cacccccggc caccacacca tcgaactcca cttgcaggcg cccaccctgc gcctgggcct
     1381 gctgatcagc ggcctcacgc tgctgctgac ggtgctgggc ggtgcggtgg ccttcctctg
     1441 gtaccgccgc acccgccgcc ccctgaaccg gagcgcgctt ctgtagtagc aggcgttgcc
     1501 ggcgccgcat gtccattaca ctcggccggc agcgacagct tgcgctggtt gctcgcctgg
     1561 ctgggggagc aggcccagtt gcaccatctg gtggaaccga tccggcacac cccagtgctc
     1621 gacgatcttg ccgttctcga agcggcacac atcaatcacc gtgattgcga aggagcggcc
     1681 cgtcggcggg cggcccatca accccttgcc gtcggtgccg cgcccagtca tccgcgccca
     1741 gaccttatcc ccgtccgcca ccatgtcctc gatggtgagg ctgaaggggg cgaaggtctc
     1801 gcgcaggaag cggatcgatc ccttaaactc ctccagcgtt gccggcagat caaattgatg
     1861 ttcggcgtag gcggggggga agcaatcatt cagggcatcg agatttcctc cattgaaccc
     1921 ttcctcgatg acccggcgaa aaacggcctt attctgttca gcggacattg cgtgcctcct
     1981 cttgcgtcgt tgtgttgatc gctgccgctg cgcaaccgca gctatcctga gtctagcgcc
     2041 agacgcaggc gcgggcatcg gggaaaacac ccatattgcg cggcggggtg cggatggggg
     2101 gaacggttca ggcgccgggg tgcgtgaggc cggtttcgac ggcgaagacg gcgatctggc
     2161 tgcgggaacc catgtcgagt ttggcgaaga tattggcgac gtgccgctcg acggtgcgtt
     2221 cgctgatcac gagcgcctcg gcaatcgcgc ggttcgtcgc gccttgtgcg atcagggccg
     2281 cgacctcgcg ttcgcggtcg gtcaggccgc cgaagcgcgc cggtggttgc tgagggccgg
     2341 cgggttcttc gccggccagg gcataggcga ccgcctgctc tactgggaga gcctgcccgg
     2401 catgccggag ttggcgccat agcggcgcgg gcaggcgggc gcgcaagtcc tccgtgtgcc
     2461 ggtcgatctc gaatcgttcg gcgggccaga tcggggcggg gtgcgccgcg cgcagggcct
     2521 cggcggcgcc gaagagccgg gcggcccggt ccagccgccc ttgcgcaagg gcgagcgccg
     2581 ccaacccgcc cagcccctcg gcgatgcccg cgggattgcc ggccgcatgc tggcggcgca
     2641 ggctctcggc
//
LOCUS       DASUYC010000435         5489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_313118,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000435 DASUYC010000000
VERSION     DASUYC010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5489
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313118"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VF276_07145"
     CDS             complement(<1..283)
                     /locus_tag="VF276_07145"
                     /inference="COORDINATES: protein motif:HMM:NF025601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HEX6606677.1"
                     /translation="MDETRKLDDSGMQGQGQSGSYTSQGQNQGQSGGYAGQSQSGGYT
                     NTGSTPNTQEFKLSGDDIMAKIKELVHEGNVRRITIKNEQGQNILEFPLT"
     gene            591..938
                     /locus_tag="VF276_07150"
     CDS             591..938
                     /locus_tag="VF276_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HEX6606678.1"
                     /translation="MIKLIALFKQPEDVAVFEQHYNDVHVPLIKKVPYMRNFVSSKAF
                     GAPRGEPAYYRVGEMWFDDRASFDKAMASDENRAAGKDLMSFARDVVTMIFVESQEIG
                     DLGPGYSATAGVE"
     gene            943..1890
                     /locus_tag="VF276_07155"
     CDS             943..1890
                     /locus_tag="VF276_07155"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX6606679.1"
                     /translation="MTATPWQGRDPAAFGFENILYSKADGIARVTINRPRVLNALNWA
                     TLREMQAAFEDASWDDNVGVLVLTGAGERAFCTGADLDEQEQFQQRPHDYWKWMGAFI
                     EAHDKLRNIGKPTIARLNGITVGGGNEFNMACDLAVAADDIFIRQVGPGRGSVPAGGA
                     TQWLPILVGDRRAREILMLCEEIPIARALDWGLVNQVVPRAELDTAVDAMAAKLLMRL
                     PTIMRYTKHQLNFWRDLSWHMTVQHARDWLSIHSDSAEVAEGLAAFHSRRPVDVPGLR
                     RAQAEAGPAGEWTCAACGTHLPPTFAFCGHCGAERSAEC"
     gene            2289..2984
                     /locus_tag="VF276_07160"
     CDS             2289..2984
                     /locus_tag="VF276_07160"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX6606680.1"
                     /translation="MRYTVYLEVQEDGATMAHVPALPGCISSGVTKEVAIARAPDAIA
                     AYHAWLRKHGEQTVPENEPIEIEIGGVIDDPDTHLGDEAGLLPTDQVALTETEAATLM
                     RLMSYSRRDLMEMVGGMSREVMHWQPQNEPSADGWCIDDILEHLARAEHVYTSRLSSN
                     VFELLDESRRAAVERMSRLSDQDMSQVTEHQGEYWTARKVFRRFLEHEREHYQHIQHV
                     LKAFESNQGLSTE"
     gene            2993..3778
                     /locus_tag="VF276_07165"
     CDS             2993..3778
                     /locus_tag="VF276_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005830716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX6606681.1"
                     /translation="MAGEPSYQFIRPRTDGAVGIVQLYRPEALNALSNALMDELIAAL
                     QAFDADEAIRCLIITGNDRAFAAGADIKDMADAGTVEMVLRDNLARWERIRAIKKPII
                     AAVSGYVLGGGNELAMTCDMIIAGDTAVFGQPEINIGVMPGAGGTQRLTRAIGKARAM
                     EMILTGRTMTAHEAYAAGLVTRVVPREVYLDEALRLARDIATKPPLAVRLAKQAVLKA
                     YELPLREGLEAERTAFYTLFSSEDQKEGMRAFIEKRKPTFQGR"
     gene            3781..4107
                     /locus_tag="VF276_07170"
     CDS             3781..4107
                     /locus_tag="VF276_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606682.1"
                     /translation="MRPDQVAREDMQAIDGLVRPLLAELADAVWGRNGRLRRKWRLTH
                     EGSTWQVTHGQPPEVFRVRVDLAQRRFQVDCHAGPIVTEGIGDEALRRALAVAHHRGP
                     AQPGEL"
     gene            4125..4907
                     /locus_tag="VF276_07175"
     CDS             4125..4907
                     /locus_tag="VF276_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009711357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX6606683.1"
                     /translation="MEYQTITWGVREGVGTLTLNRPEVLNAFNRQMTDEIQDVLKQAE
                     RDKAVRCLVITGAGRAFSSGEDLKAHRDSGPGSIAEAVRTRYNPIIMKMRALEKPIIA
                     SVNGVAAGAGCSLALAADLRIASDKARFIEAFSRIGLVPDSGSTFFLPWLVGYSKAAE
                     LCFLAEDVPADEALRIGLVNKVVPADELEAATAAWATKLAQGPTVAYGLTKRALNRAL
                     HAPLHEILEYEMYGQEAAGRTADFREAVAAFLEKRPATFKGE"
     gene            4910..>5489
                     /locus_tag="VF276_07180"
     CDS             4910..>5489
                     /locus_tag="VF276_07180"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX6606684.1"
                     /translation="MTTTEPGLVGVIGAGTMGAGIAQVAAQSGRPVLLYDIKPEFVER
                     GLGTITRGLQGRVDKGRMAAADMAAILDRIRTTTDRDALAPAAVVIEAAPESLALKQE
                     LFAALDQMCGPDTILASNTSSLSITALGAATKRPERVAGMHFFNPAPVMALVEVVAGQ
                     RTDPAVAAAIVDLARAMGKTPVQARDTPGFIVN"
BASE COUNT          943 a         1779 c         1901 g          866 t
ORIGIN      
        1 cggtcagcgg gaattcgagg atgttctgcc cctgttcgtt cttgatggtg atgcggcgga
       61 cattgccttc gtggaccagt tccttgattt tcgccatgat gtcgtcgccg ctgagcttga
      121 actcctgcgt gttgggagta gaaccagtat tcgtgtaacc ccccgactgg ctctggcccg
      181 cataaccccc ggattggccc tggttctggc cctggctcgt ataactcccc gattggccct
      241 ggccctgcat tcccgagtca tccagtttgc gtgtctcgtc cattagagtt gctcctttcc
      301 actgcatacc ggtggcagcc agcgccggcg tgggtggtcg ccggacgccc ggtgccgtca
      361 cggcacgaat ccccgatccg aatcgaggac agtctgtaaa gtcagctaat tacgtgccat
      421 ggatccgggc gggcgtgggg gccgggcgcc gccagggtac gtgccttgac agggatgggg
      481 gcctgtggta ttttagcggg gccacgaccg cgttaccccc cgccccggcc accctgccgg
      541 gccgccgccg ggccggcttc cgcgccacac aaggaggaca cactaccgcg atgatcaagc
      601 tgattgctct gttcaagcag ccggaggacg ttgccgtctt cgagcagcac tacaatgatg
      661 tgcatgtgcc gctgatcaaa aaggtgccct acatgcgcaa cttcgtttcc agcaaggctt
      721 ttggggcgcc ccgcggcgaa ccggcctact atcgcgtcgg cgagatgtgg ttcgacgacc
      781 gggccagctt cgacaaggcc atggcctccg acgagaaccg tgccgccggc aaagacctga
      841 tgagcttcgc ccgcgacgtg gtgaccatga tttttgtgga gtcgcaggag atcggcgacc
      901 tcggccccgg ctacagtgcg accgcggggg tggagtaacc gcatgaccgc gactccctgg
      961 cagggccgcg acccggcggc ctttggcttc gagaatatcc tctacagcaa ggcggacggc
     1021 atcgcccgcg tcaccatcaa ccgcccgcgc gtgctcaacg ccctcaactg ggccaccctg
     1081 cgcgagatgc aggcggcctt cgaggatgcg tcctgggacg acaacgtggg cgtgctggtg
     1141 ctgaccgggg cgggcgaacg cgccttctgc accggcgccg acctcgacga gcaggagcaa
     1201 ttccagcagc gcccgcacga ttattggaag tggatggggg cgttcatcga ggcccacgat
     1261 aagctgcgca acatcggcaa gccgaccatc gcccggctga acggcatcac cgtgggcggg
     1321 ggcaacgagt tcaacatggc ctgcgacctg gccgtggccg ccgacgacat cttcatccgc
     1381 caggtgggac cgggccgggg cagcgtgccc gcgggcggcg ccacccagtg gctgccgatc
     1441 ctggtgggtg accggcgcgc ccgcgagatc ctgatgctgt gcgaggagat tccgatcgcc
     1501 agggcgctcg actgggggct ggtgaaccag gtggtgccgc gcgccgaact cgacacggcg
     1561 gtcgacgcca tggctgccaa gttgctcatg cgtttgccga cgattatgcg ttataccaag
     1621 caccagttga acttctggcg cgacctgagc tggcatatga cggtgcagca cgcgcgcgac
     1681 tggctgagta tccactcgga ctcggcggaa gtggccgagg ggctggccgc cttccacagc
     1741 cgccgcccgg tggacgtgcc gggcctgcgc cgcgcacagg ccgaagccgg tcctgcgggc
     1801 gagtggacgt gcgccgcctg cggcacccac ctgccgccca cgttcgcctt ctgcggccac
     1861 tgcggcgcgg agcgaagtgc tgagtgctga gtacgacgag tggtcagtgg tcaggggtca
     1921 gtggccagta acgagttaga cttgtcactc tgagcgcagc gaagagtctc ggcgcctgcg
     1981 ggttacgagc tggagattct tcgctccgct cagaatgaca ctgggggctt gtctagggag
     2041 cgcagcgaag agtctcggcg cctgcgggaa caagacgaga tgcttcactc cgttcagcat
     2101 gacaacttgg ggtggccggt ggtcatcagt tgatttgctt caacggtagc tgttctatat
     2161 tgccaatcgc ggcttaggaa acggttgggc aggcttgcgg tgtccgggac gacgagcgac
     2221 cgtccctgac tactgaccac tgatcactga tcactgacca ccgacggctg acgcagagga
     2281 gccaggggat gcgctatacg gtttatctgg aggtccagga ggatggggcg acgatggccc
     2341 acgtgccggc gttgcccggc tgcatcagca gcggcgtgac caaggaagtg gcgatcgccc
     2401 gcgcgcccga cgccatcgcc gcctatcacg cgtggctgcg caagcatggc gagcagacgg
     2461 tgccggagaa tgagccgatc gagatcgaga tcggcggcgt gatcgacgat cccgacaccc
     2521 acctgggcga tgaagccggg ctgctgccca ccgaccaggt ggcgttgacc gagaccgagg
     2581 ccgccacgtt gatgcgcctc atgtcctatt cccgccgcga cctgatggaa atggtcggcg
     2641 ggatgtcgcg cgaggtgatg cattggcagc cccaaaacga gccgagcgcc gacggctggt
     2701 gcatcgacga catcctggag cacctggccc gcgccgagca tgtctacacc agccgcctga
     2761 gcagcaacgt ctttgagttg ctcgatgaat cccgccgggc tgccgtggag cgcatgtcgc
     2821 gcctgagcga ccaggacatg agccaggtca ccgaacacca gggcgaatac tggacggcgc
     2881 gcaaggtgtt ccgccgcttc ctggagcacg agcgggaaca ctaccagcac atccagcacg
     2941 tgctgaaggc gttcgagtcc aaccagggtt tgagtacgga ataggagcac gtatggccgg
     3001 cgagccgtct taccagttta ttcgcccacg taccgacggt gcagtgggca tcgtccagtt
     3061 gtaccgcccc gaagcgctca acgcgctcag caacgcgctg atggacgaac tgatcgccgc
     3121 gttgcaagcc ttcgacgccg acgaagcgat ccgctgcctc atcatcaccg gcaacgatcg
     3181 cgccttcgcc gccggggccg acatcaagga tatggcggac gcgggcacgg tcgagatggt
     3241 gctgcgcgac aacctggccc gctgggagcg catccgggcg atcaagaagc cgattatcgc
     3301 cgccgtcagc gggtatgtgc tgggcggggg caatgaactg gcgatgacct gcgacatgat
     3361 catcgccggc gacacggccg tcttcgggca gccggagatc aacatcggcg tgatgccggg
     3421 ggcaggcggc acgcagcgcc tcacccgcgc catcggcaaa gcccgcgcca tggagatgat
     3481 cctgaccggg cgcacgatga cggcccacga agcctacgcg gcaggcctgg taacgcgcgt
     3541 ggtgccgcgc gaggtctacc tggacgaggc gttgcgcctg gcccgcgaca tcgcgaccaa
     3601 gccgccgctg gccgtgcggc tcgccaagca ggccgtgctc aaagcctacg agctaccgct
     3661 gcgcgagggc ctggaagccg agcgcaccgc gttttacacc ctgttcagca gcgaggatca
     3721 gaaggagggc atgcgcgcgt ttatcgagaa acgcaagccc accttccagg gccgctgatc
     3781 atgcgccccg atcaggtcgc gcgcgaggac atgcaggcca tcgacggcct ggtgcgcccg
     3841 ctgctggccg aactggccga cgccgtctgg gggcgcaacg ggcgcttgcg gcgcaagtgg
     3901 cgcctcacgc acgagggatc gacctggcag gtgacccacg ggcagccgcc ggaggtcttt
     3961 cgcgtgcggg tcgatctcgc gcagcggcgg ttccaggtgg attgccacgc cgggccgatc
     4021 gtcaccgagg gcatcggcga cgaggcgctg cggcgcgccc tggctgtggc ccatcaccgc
     4081 gggccggcgc agcccggcga actctaagcc aacaggaggc gagtgtggag tatcagacga
     4141 ttacctgggg agtgcgcgag ggcgtgggca cgctgacgct caaccggccc gaggtgctca
     4201 acgcctttaa ccggcagatg accgacgaga tccaggacgt gctcaagcag gccgagcgcg
     4261 ataaagccgt gcgctgcctg gtcatcaccg gggccggacg cgcgttttcg tcgggcgagg
     4321 acctgaaggc gcaccgcgac agcgggccgg ggtcgattgc ggaggccgtg cgcacgcgct
     4381 acaacccgat tattatgaag atgcgcgcgc tggagaagcc gatcatcgca tccgtcaacg
     4441 gggtggccgc cggggcgggg tgcagcctgg cgctggccgc cgacctgcgc atcgcgtcgg
     4501 acaaagcccg gttcatcgag gcgttctcgc gcatcgggct ggtgccggac tcgggcagca
     4561 cgttcttcct gccctggctg gtgggctaca gcaaggccgc cgagttgtgc ttcctggccg
     4621 aggatgtgcc cgccgacgag gcgctgcgca tcgggctggt caacaaagtg gtgcccgccg
     4681 acgaactgga agcggccacg gcggcctggg ccaccaagct cgcccagggc ccgaccgtcg
     4741 cctacgggct gaccaagcgg gcgctgaacc gcgcgctgca cgcgccgctg cacgagatcc
     4801 tggagtacga gatgtacggc caggaagcgg cggggcgcac cgctgatttc cgcgaggcgg
     4861 tcgccgcctt cctggagaag cggcccgcga cgttcaaggg cgagtaaccg tgacgacgac
     4921 agagccgggc ctggtcggcg tgatcggcgc gggcacgatg ggcgcgggga tcgcgcaggt
     4981 ggccgcgcaa agcgggcgcc ccgtgctgct gtacgacatc aagcccgagt ttgtcgagcg
     5041 cggcctgggc acaatcacgc gcgggttgca gggccgggtg gacaagggcc gcatggccgc
     5101 cgcggacatg gcggctatcc tcgaccgcat ccgcacgacg accgaccggg acgccctggc
     5161 cccggcagcc gtcgtgatcg aagcggcgcc ggagagcctg gcgctgaagc aagaactgtt
     5221 cgccgcgctc gaccagatgt gcggccccga cacgatcctg gcctccaaca cgtcgtcgct
     5281 gtcgatcacg gcgctgggcg ccgctacgaa gcgcccggag cgcgtcgccg ggatgcactt
     5341 cttcaacccg gcgccggtca tggccctggt cgaggtggtc gccgggcagc gcaccgatcc
     5401 ggcggtggcg gcggcgattg tggacctggc gcgcgccatg ggcaagacgc ccgtgcaggc
     5461 gcgcgacacg cccggcttca tcgtcaatc
//
LOCUS       DASUYC010000436         2287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1228336, whole genome shotgun sequence.
ACCESSION   DASUYC010000436 DASUYC010000000
VERSION     DASUYC010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228336"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="VF276_07185"
     CDS             complement(<1..711)
                     /locus_tag="VF276_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606685.1"
                     /translation="MTWPIAAHLTRTLPDGGDDWRFLWNLWWMKTALVDLHTNPFHTT
                     YLYYPQGANLYIDTFAPALGVLSLPLQLLGLPLVAVYNLLALGSFVAAGYGTYLLVRY
                     LTGRRAPAFVAGLIFAFCPYHFAHLLGHLNLVSLQWMPFYVLALVRTWEWPQPAGRRA
                     GDTPAPSARRAPVWWAALAGVWLAITAYTEWTYALFLGLFTLWYLAWVLLVVRGGPGR
                     RAAGARLAVLAGIWLGLVA"
     gene            complement(792..1583)
                     /locus_tag="VF276_07190"
     CDS             complement(792..1583)
                     /locus_tag="VF276_07190"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX6606686.1"
                     /translation="MHIPSGSTTSDLHSGARRRRLRRALPGLLLLILALLLVPAPAGA
                     SYPSPRPSNEPAAARYQGQVTRIAPGKKRIGLTFDGDAFGGRTGEIIDDLYRYHAKAT
                     FFLTGKYIEMYPTRSRGLVNSGQEVASHGYKHLDYRDLSDGGIARQLAAWEATYSWLT
                     GGKHGPPIWRAPYGYSTNRVRVVAADSGYRTIYWTLDALDTVGAPKSASFIFNRLTRP
                     AINLDGAILLLHVNPPGTIDALPRVLANLQARGLQMVTVSELLAP"
     gene            1582..>2287
                     /locus_tag="VF276_07195"
     CDS             1582..>2287
                     /locus_tag="VF276_07195"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6606687.1"
                     /translation="MAVHLETIAPGVLYLPGAQNIGIIPMDRDRGAIVIDTGLTRDTG
                     KAILKALDAAGLRPAAILNTHAHADHFGGNAFLVEKTGVPVYAPVYEEAFLRYPRLEA
                     AGLFAGAAPIAALQGRFLLAPASPVDHIVTPGPLSIDGVPLEAVPLPGHSAGQTGYLV
                     RDVLFSGDLVMPPAVLDKYGIVYCHDVAAHLESLERVPALAATWHLPAHHPPTRDLAP
                     LVAANRDRFQDTLAQIR"
BASE COUNT          411 a          752 c          792 g          332 t
ORIGIN      
        1 agcgaccagc ccgagccaga tccctgccag caccgccagc cgggcgcccg ccgcgcgcct
       61 gcccggtccg ccgcgcacca ccagcagcac ccaggccagg taccagaggg tgaacagtcc
      121 gagaaacagc gcataggtcc actcggtgta agccgtgatc gccagccaca ccccggccag
      181 ggccgcccac cacacgggtg cccggcgggc ggatggcgcc ggggtatccc ccgccctcct
      241 gcccgccggc tgcggccatt cccaggtccg cactaacgcc agcacataga agggcatcca
      301 ttgcagggag acgagattca ggtggcccag caaatgcgcg aagtgatagg ggcagaaagc
      361 gaagatcagc ccggcgacaa aggcaggagc ccgccgtccg gttaagtagc gcaccagcag
      421 ataggtgccg tagcccgcgg cgacaaaact acccagggcc agcaggttat agacggcgac
      481 caggggcagg cccagcaact ggaggggaag gctcagcacg ccgagcgcgg gggcgaaggt
      541 atcgatatag agattagcgc cttgcggata atagaggtac gtggtgtgaa acggattggt
      601 gtgcagatcg accagcgccg ttttcatcca ccagaggttc cagaggaagc gccaatcatc
      661 gccgccatcc ggcaaggtgc gggtgagatg ggcggcaata ggccaggtca atacgagggt
      721 taacccggta tagatcgcca atatggcaac ggtttcgcgc aagcgcccgg tcaggggcgc
      781 tgccgcaccg gtcaaggggc cagcagctcc gagaccgtga ccatttgcag cccgcgggcc
      841 tggaggttcg ccagcacgcg gggcagcgcg tcgatggtgc cgggcggatt aacgtgcagc
      901 agcaggatcg cgccgtcgag gttgatagcc ggacgggtca gacggttaaa gatgaagctg
      961 gcgctcttgg gggcgcccac ggtgtcgagt gcgtccagcg tccaataaat cgtgcggtaa
     1021 ccggagtcgg cggcgacgac gcgcacacgg ttggtactgt agccgtaggg cgcgcgccag
     1081 atgggcgggc cgtgcttacc gccggtcaac caggagtatg tcgcctccca ggctgcgagc
     1141 tggcgggcga tgccgccgtc gctcaggtcg cggtagtcga ggtgcttgta gccatgcgag
     1201 gcgacctctt gcccggagtt gacaagcccg cgcgatctgg taggatacat ctctatatac
     1261 ttcccggtga ggaaaaaggt ggccttcgcg tggtagcgat agaggtcgtc gatgatttcg
     1321 ccggtgcgcc cgccgaaggc gtcgccgtca aaggtcagcc cgatgcgctt cttgcccggc
     1381 gcgatgcgcg tgacctggcc ctggtagcgg gccgccgcgg gttcgttgct gggccggggc
     1441 gagggatagg aggcgccggc cggcgcgggc accagcagca gcgccagaat cagcagcaac
     1501 aggccgggaa gtgcccggcg caggcgccgc cggcgggcgc cggagtgcag gtcagatgtc
     1561 gtggaaccgg aaggaatatg catggctgtc catcttgaaa cgatagctcc gggggtcttg
     1621 tacctgccgg gagcgcaaaa tatcggtatc atccccatgg accgggaccg cggggcgatc
     1681 gtgatcgaca ccggcctgac ccgcgatacc gggaaggcca tcctgaaggc gttggacgcc
     1741 gccgggttgc gccccgcggc catcctcaat acccacgccc acgccgacca cttcggcggc
     1801 aacgccttcc tggtggaaaa gaccggcgtg ccggtctacg ccccggtcta tgaggaagcg
     1861 ttcctgcgct acccgcggct cgaagcggcc ggcctcttcg ccggggcggc gccgattgcc
     1921 gcgctacagg gccgcttcct gctggcgccc gcctcgcccg tcgatcacat cgtgacgccg
     1981 gggccgctga gcatcgacgg tgtgcccctg gaagccgttc cgctgccggg gcactccgcc
     2041 gggcagacgg gctacctcgt gcgcgatgtg ctgttcagcg gcgacctggt gatgccgccc
     2101 gcggtgctgg acaaatacgg catcgtctac tgccacgatg tcgccgccca cctggagtcg
     2161 ctggagcgcg tgccggcgct cgcggccacc tggcatcttc cggcccacca tccgcccacc
     2221 cgcgacctgg cgcccctcgt cgcggccaac cgggaccgct tccaggatac cctggcccag
     2281 atccggc
//
LOCUS       DASUYC010000437         4204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1925075, whole genome shotgun sequence.
ACCESSION   DASUYC010000437 DASUYC010000000
VERSION     DASUYC010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1925075"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VF276_07200"
     CDS             complement(<1..397)
                     /locus_tag="VF276_07200"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX6606688.1"
                     /translation="MKDEKPFAVPTVNGPEPPKPVGWQISTSTVIRIMLVILGVLFAA
                     WAIYRILPILLLLLIAIMLATAIEPVVNNLRRGPFSRSQGILIVYTGIFIVLLVIGWL
                     IVPVFINQVGEVLNNLPRSISDFKTWAGTI"
     gene            complement(848..1135)
                     /locus_tag="VF276_07205"
     CDS             complement(848..1135)
                     /locus_tag="VF276_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606689.1"
                     /translation="MSCGCGELDNNHGDPANITLGQMQRAAQAANIDVEHAADNIHHA
                     ARRYRDQQGRHGTEMHKENMAGGNYRTGDMLGTTSAAPNRDTTTDTNRGTL"
     gene            1230..2288
                     /locus_tag="VF276_07210"
     CDS             1230..2288
                     /locus_tag="VF276_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6606690.1"
                     /translation="MAAVIAGLARPLDSDGALLYADGTMENTNATTQTTGSIPAPAEA
                     AAARAGARAAWLDPGVLWLLASAAGFGSMAIFAKLAYSGGLTVPTLLATRFGLAAILM
                     WGLLAARRLSPRVPPRTLVGLLLMGGIGYVGQSFAFFTALETIPAATTALLLYTYPAM
                     VSVLAWVILKQRLSRTAALALALATAGCALVVGGPAALPAQVQIDPAGMAWGLAAALI
                     YSLYIIAGTRVTANVPPLVASTYIISAAAGVFLGAGTLGGTLNFAVDAGAWAAMIGIA
                     LICTVLAIAAFFAGLARLGPGRASILSTVEPIVTFLLAALVLGEAISLIQIAGGALIL
                     SAVLVLQWPRKAARPAAL"
     gene            complement(2312..3247)
                     /locus_tag="VF276_07215"
     CDS             complement(2312..3247)
                     /locus_tag="VF276_07215"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6606691.1"
                     /translation="MAHVMAAGMAARRAALWRALGRLVVALAVFSLVSYIAVSAYAAD
                     RFSHPIRQPVGQCRDYGLTCEDVQFPSTVDNVPLRGWFIGGTGPRTILMLHGRDGRRD
                     DPAIGLMAIAQAFHAHGYSLLMFDFRAHGLSGGDRYSLGDWERRDVAGALAYLRGRGI
                     AGVGVIGFSMGAGTALRAAPAHPEMRALVLDSPFADVPALVDQHLTEVSGLPRFFTPG
                     ILLMAQGVYGMDLLDDRPVQEMATLGTRPILLIHSTTDDYIPVSQAYELQRAGAHDPN
                     LQVWFTTGPTHVRSFKEYPTEYLRRVIGFFDANLP"
     gene            3610..>4204
                     /locus_tag="VF276_07220"
     CDS             3610..>4204
                     /locus_tag="VF276_07220"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX6606692.1"
                     /translation="MELPLTPTRFLNRAVALFPDKPAVINGDARFTYREFKERSNRFG
                     HAMSGLGLGRGAVIAYLGLNNHQILEAYYGVLPSGFVLLPLNVRLRPQDLAYILADAD
                     AKALIVGPEFAQLGFGLREAVPGLQHLIMLGEDVPVNALSYERLLADAAADDIDYMQV
                     DEHDVAEMFYTSGTTGRPKGVELTHRNLYLHALSCIAS"
BASE COUNT          698 a         1421 c         1393 g          692 t
ORIGIN      
        1 ggatggtgcc cgcccaggtc ttgaagtcgc tgatgctcct gggcaggttg ttaaggactt
       61 cgcccacctg gttgatgaac accgggacaa tcagccagcc gatcaccagc agcacgatga
      121 agatgccggt atagacgatc aggatgccct ggctgcggct gaaaggcccg cgccgcaggt
      181 tgttgacgac cggctcgatg gccgtcgcca gcatgatagc gatcaagagc aggagcagga
      241 tcggtaagat gcgatagatg gcccaggcgg cgaagagtac gccgaggatc accagcataa
      301 tgcggatcac cgtgctggtg gagatctgcc accccaccgg tttgggcggc tcgggcccgt
      361 tcaccgtcgg cacggcgaac ggtttctcat ctttcatttc ggtcccgtac agatggacct
      421 cggagttgtc ggattgcatc aggcgcctca gcttgtaggt aatggaattt gcgggccagg
      481 cactgagcac gcgccgctcc agccggcacc cggacgatca gcgcaggccg caccggcgcg
      541 gcgaccggcc cccggcggga tcgggggatc gggccagccc ggccaacgag gaagcgagaa
      601 agcggccggt gctacttgcc ggcagacggc cactctggtt gtgcgctgtc ggggccggtt
      661 tgaaaccggc ctgccgcccc atcgaatcag ggtggtggac tactggctgc tgagggtcgc
      721 aaaagcaaac aacgtgccat ccgccaggca cacgcaaacc acccggtgcg cccaggcggt
      781 gcgtcgagga gaggcgggca cccgcggcgc gagagggggc gccggcgggg gccacagagc
      841 ggcagaatta gagggtgccg cggttggtgt cggtcgtcgt gtcgcggttg ggcgcggcgc
      901 tggtcgtgcc cagcatgtcg ccggtgcgat agttgccgcc ggccatgttc tccttatgca
      961 tctcggtccc gtgccggcct tgctgatcgc gataccgccg cgccgcgtgg tggatgttgt
     1021 ctgccgcgtg ttccacgtcg atattggcgg cctgcgccgc ccgctgcatc tggccgagcg
     1081 tgatattcgc cggatcgccg tggttgttgt ccagttcgcc acagccgcaa ctcatacaca
     1141 tatctgcgct cctttcgctt gccccgcgcc ggcgacgggt gccgttcgcg cacgggtaac
     1201 actcgaaata caagcagaaa gagtgccata tggcagccgt aattgctggg ctcgcccgcc
     1261 cgcttgacag cgacggcgcg ctattgtacg ctgacgggac gatggagaat acgaacgcca
     1321 cgacacaaac gacaggtagc atcccggcgc ccgccgaggc tgcggcggcg agggccggcg
     1381 cgcgggcggc ctggcttgat cccggcgtgc tctggctgct ggcctcggcg gcgggctttg
     1441 gctcgatggc gatcttcgcc aagctggcct acagcggggg cctgaccgtg ccgacgctgc
     1501 tcgccacgcg cttcgggctg gccgccatcc tgatgtgggg cctgctggcc gcgcgccgcc
     1561 tctcgccacg cgtgccgccc cggacgctgg tcgggctgct gctcatgggc ggcataggct
     1621 acgtgggcca atcgttcgcc ttcttcaccg cgctggagac aatccccgca gcgacgaccg
     1681 cgctgctgct ctacacctac ccggccatgg tcagtgtgct ggcctgggtg atcctcaagc
     1741 aacggctcag tcgcacggcg gcgctggccc tggcgctcgc cacggcgggc tgcgcgctgg
     1801 tggtgggcgg ccccgcggcg ctgcccgccc aggtgcagat cgaccccgcg ggcatggcct
     1861 gggggctggc cgccgcgctg atctactcgc tctacatcat cgccggcacg cgcgtcacgg
     1921 ccaacgtccc gcccctggtc gcctcgacct atatcatcag cgcggcggcg ggagtcttcc
     1981 tcggcgcggg cacgctcggc ggcacgctca acttcgccgt cgatgccggc gcctgggcgg
     2041 ccatgatcgg catcgccctg atctgcaccg tgctggcgat cgccgccttc ttcgcgggcc
     2101 tcgcccgcct gggaccgggc cgggccagca tcctcagcac cgtggagccg atcgtcacct
     2161 tcctgctggc ggccctggtg ctcggcgaag ccatcagcct catccagatc gcgggcggtg
     2221 cgctgatctt gagcgccgtg ctggtgctgc aatggccgcg caaagccgcg cggcccgccg
     2281 cgctatagcc ccccggcgag cgcgactact gctatggtag attcgcgtca aaaaacccga
     2341 tcacgcgccg gagatattcg gtggggtact ccttgaagga gcgcacgtgt gtaggtccgg
     2401 tcgtgaacca gacctggaga ttgggatcat gtgcccccgc ccgctggagt tcataagcct
     2461 ggctcaccgg gatgtaatcg tcggtcgtgc tgtggatcaa gaggatgggg cgcgtgccga
     2521 gcgtcgccat ttcctgcacg ggccggtcgt ccagcaggtc catgccgtat actccctggg
     2581 ccatcaacag gataccgggg gtgaaaaagc gcggtaagcc gctgacctcg gtcagatgct
     2641 ggtcgaccag cgccggcacg tccgcgaacg gcgagtccag caccagggcc cgcatttcgg
     2701 gatgggcggg cgccgcacgc aacgccgtgc ccgcgcccat cgagaacccg atcaccccca
     2761 cgccggcaat cccccgcccc cgcaagtagg ccagcgcccc ggcgacatcc cggcgttccc
     2821 agtcgcccag cgagtagcgg tcgccgccgg agaggccgtg cgcgcgaaag tcgaacatca
     2881 agaggctgta gccatgcgcg tggaaggcct gcgcaatcgc catcaggccg atggccggat
     2941 cgtcccgccg gccatcgcgg ccatgcagca tcaggatggt gcgcggcccc gtgccgccaa
     3001 tgaaccagcc gcgcaacggc acgttatcca ccgtactcgg gaactgcaca tcctcgcacg
     3061 tcagcccgta gtcgcggcat tggccgacgg gctggcggat aggatgggag aaccgatcgg
     3121 cggcgtaagc ggagacggcg atatagctta cgagactgaa aacagcgagc gccaccacga
     3181 ggcggccgag cgcccgccac agcgcggcgc gccgcgccgc cataccggcg gccatcacgt
     3241 gggccatggt ctctatctcc ttgccgcgcc ggtagtccag cacagtaagg ccggcgaccg
     3301 gcaaggtcgg gtttcaaccc ggtcctgacg gtcacaaata ctgtagccgc ctgccagtga
     3361 ggtcgaagac cagcgcacca accacgcccg gtctgttgta gccgcgtaca cgctgcgtgc
     3421 ccaatatgat gataacacac atacgcaagg tccgccgatt ccgatacaga ccccggcggg
     3481 ccgggcgggt gcggggtcgc ttgacagcct ctgccgggca ttctatactg cttatacatc
     3541 gcgcaatcac gccccatcca ccggttgcag cccagatcct accattccca gaatccgcgg
     3601 aggtgaacaa tggaactgcc cctcacgcct acccgtttcc tcaatcgggc cgtcgcgctt
     3661 ttcccggaca agccggcggt catcaacggc gacgcacgtt tcacgtatcg tgaattcaag
     3721 gagcggtcca accggttcgg gcatgccatg agcggcctcg ggctgggccg gggcgcggtc
     3781 atcgcctacc tgggcctgaa caaccaccag atcctggagg cgtattacgg cgtgctgccg
     3841 tcgggcttcg tactgctgcc gctgaacgtc cggctgcgcc cgcaagacct ggcctatatc
     3901 ctggccgacg ccgacgccaa agcgctcatt gtcgggccgg agttcgcgca actcggcttc
     3961 gggctgcgcg aggcggtgcc ggggctccag cacctgatca tgctcggcga ggatgtgccg
     4021 gtcaatgcgc tcagctatga gcgcctgctg gcggacgcgg ctgccgacga catcgactac
     4081 atgcaggtgg acgaacacga tgtagccgag atgttttaca ccagcggcac gacgggccgg
     4141 cccaaaggcg tggaactgac ccaccgcaat ctctacctgc acgccctgag ttgtatcgcg
     4201 tcct
//
LOCUS       DASUYC010000438         4157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1644819, whole genome shotgun sequence.
ACCESSION   DASUYC010000438 DASUYC010000000
VERSION     DASUYC010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1644819"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..164
                     /locus_tag="VF276_07225"
     tRNA            80..164
                     /locus_tag="VF276_07225"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:114..116,aa:Tyr,seq:gta)
     gene            191..264
                     /locus_tag="VF276_07230"
     tRNA            191..264
                     /locus_tag="VF276_07230"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:225..227,aa:Pro,seq:tgg)
     gene            270..345
                     /locus_tag="VF276_07235"
     tRNA            270..345
                     /locus_tag="VF276_07235"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:306..308,aa:Arg,seq:gcg)
     gene            383..457
                     /locus_tag="VF276_07240"
     tRNA            383..457
                     /locus_tag="VF276_07240"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:415..417,aa:Gly,seq:gcc)
     gene            461..535
                     /locus_tag="VF276_07245"
     tRNA            461..535
                     /locus_tag="VF276_07245"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:493..495,aa:Asn,seq:gtt)
     gene            566..700
                     /locus_tag="VF276_07250"
     tRNA            566..700
                     /locus_tag="VF276_07250"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            821..2638
                     /locus_tag="VF276_07255"
     CDS             821..2638
                     /locus_tag="VF276_07255"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEX6606693.1"
                     /translation="MDLKPITVYNWKDYAVPHPDGYFTLQTRDTGDVPVRLFFTPALL
                     AEAEDGLYPQIVNATRFPGVKLVVITPDAHFGYGVPVGSVILTGGTVAMGPVGYDIGC
                     FTGDTLIPTVDGRAYPIADLVDAAGELLVYALDADQQIVVARATARRTRRAAPLVKVV
                     LDNAQTIVCTPDHLFLLPNGVYRQAQHLHPSTVLMPFRLPPDRPALKVVAVEPLAYTA
                     DVYCLAVPDYGNFALAAGVFVHNCGMISARSDVPVGAANRDKRLAFNRAVTERIALGA
                     GGTSKTLGRIGEKEFQKLIRGGADYYLSKYGAGLDRSRAERNRIPVDDDWDVPWGGKG
                     RPERGMNQLGSLGGGNHFIELQRCEETGTLFVQVHSGSRGFGHGLATNYFELARGNKG
                     KKAAHIDLGYFTHDSPYHRSYLNAVAAGGNFAIINRLILFQQLAEAFEEVFGAELELI
                     YEISHNLVQREEHPEFGEVWVHRKGATRAFPAGHPALAGTRWADTGHPVLIPGSNRDF
                     SYILRPLPGAVNSGYSVNHGAGRRLSRSAAKQKLSQEQVNREYREAGIIVNIDGKVPL
                     DESVACYKSAAEVVDAVVQAGLAEVEYRLWPLASLKGTD"
     gene            2802..3293
                     /locus_tag="VF276_07260"
     CDS             2802..3293
                     /locus_tag="VF276_07260"
                     /inference="COORDINATES: protein motif:HMM:NF021733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606694.1"
                     /translation="MDERHEHEQYFFDAATLAHLAGVVAAYPNPCCLCAPRLGQELER
                     RGVAVRTLDIDTRFAGLAGFRRYDVFRPEWLGEPFGLILCDPPFFRVSLAQLFTAIRL
                     LSRYDYRQPLLISYLSRRAANIRGTFARFDLRPTGYYPGYLTVQNVERNGIEFFGNLA
                     HPI"
     gene            3397..4026
                     /locus_tag="VF276_07265"
     CDS             3397..4026
                     /locus_tag="VF276_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606695.1"
                     /translation="MTRSPYLSPRPPLDSTPATLTAFERLYREAVAPGTGQPIDYTLA
                     APKWQFLCWLCETQAILLHGSGDPGIAEFEPRQSNDIEEFGNRQAVYAASDGIWPIYF
                     AVMDRDRYVRSLMNGCVREVAPDGTRGEPYYFFSINGDALPHQPWRQGTIYLLPRATF
                     EQQPLLEKGGSGSRWRSGPAWCRCGPWPGWPSGRMTSRSYRRSLPMTRP"
BASE COUNT          646 a         1450 c         1379 g          682 t
ORIGIN      
        1 cgccgagcag cgcaacgcgg gatcggtctc gcgcccgttc tgatcgccgc cggctgccgc
       61 gataatagag ctaacacgag cgcagatgcc ggagcggccg atcgggcctg actgtaaatc
      121 aggtggcatt gccttcggag gttcaaatcc tcctctgcgc accacgccgg gccggcccgg
      181 cgcccagctt tggcatgtgg cgcagcctgg tagcgcacct gccctgggag cagggggtcg
      241 cccgttcaaa tcgggccatt ccaaccccac ggggtgtagc tcagcggatc gcagagcgcc
      301 gggccgcgaa cccggaggcc gcaggttcga ttcctgccat cccgaccgta tcggaattgg
      361 ggcgtcgcac ggcgcccgcc ttgcagaagt agctcaacag cagagcgtgt gcgtgccacg
      421 tacgaggctg cgggtgcgag tcccgccttc tgctccattt tggcctgtgg cgcaacggta
      481 gcgcgaggag ctgttaactc cgcggttgca ggttcgaatc ctgccgggcc agccaggaac
      541 tgcacgggga ccgggccggg tgatcggcgg tcgtggcgga aacggcaaac gcgctacgtc
      601 agccgctttt tcatcatttg cccggcgctg gccgtcgaaa gagcgttatc gaaacatagt
      661 aaccggcagg tttttgggag ttcgagtctc cccgccgccg ccttgttcgg ttcccggtgg
      721 ttccctcatc tcgcgttcgc gggcgcggcg cgcgtggcgc gttccatgca gtcttcgtcg
      781 tcgacatagc acaccacatc taccacgagg aaggggagtc atggacctga agccgatcac
      841 cgtctacaac tggaaggact acgccgtgcc ccacccggac ggctacttca cgttgcagac
      901 ccgcgacacg ggcgacgtgc cggtgcggct cttcttcacc ccggccctgc tggccgaggc
      961 cgaagacggc ctctacccgc aaatcgtcaa cgccacccgc ttccctggcg tgaagctggt
     1021 cgtcatcacg cccgacgccc atttcggcta cggcgtgccg gtcggctcgg tcatcctgac
     1081 cggcggcacg gtggcgatgg ggcccgtcgg gtacgatatt ggctgcttca ccggggacac
     1141 gctgatcccg accgtggatg ggcgcgcgta ccccatcgcg gatctcgtgg acgcggcagg
     1201 ggaactgctg gtctatgcgc tcgacgccga tcagcagatt gtcgtggcgc gggccacggc
     1261 ccgccggacc cggcgcgccg cgccgctggt caaggtcgtg ctggacaacg cgcagacgat
     1321 tgtctgcacg ccggatcatc tctttctgct gcctaacggt gtctaccggc aagcccagca
     1381 cctgcatccc agtactgtcc tgatgccttt ccgtttgccc cccgaccgcc cggctctcaa
     1441 agtagtcgcc gtggagccgc tggcgtatac ggcggatgtc tattgcctgg ccgtgcccga
     1501 ctacggcaac tttgcgctgg ccgctggcgt cttcgtgcat aactgcggca tgatcagcgc
     1561 gcgctcggac gtgccggtgg gcgcggcgaa ccgcgacaag cggctggcct ttaaccgcgc
     1621 cgtcaccgag cgcatcgcgc tgggtgcggg cggcacgagc aagacgctgg gccgcatcgg
     1681 cgagaaggag ttccagaagc tgatccgcgg cggcgccgac tactacctct cgaagtacgg
     1741 cgcggggctg gaccgcagcc gcgccgagcg caaccgcatc ccggtggacg acgattggga
     1801 tgtgccgtgg ggcggcaagg gccgcccgga gcgcggcatg aaccagttgg ggtcgctggg
     1861 cggcggcaac cacttcatcg aactgcaacg ctgcgaggaa acgggcacgc tgttcgtgca
     1921 ggtccatagc ggcagccgcg gcttcggcca cggcctggcg accaactact tcgagctggc
     1981 gcgcggcaac aagggcaaga aggcggcgca tatcgacctg ggctacttca cgcacgactc
     2041 gccgtaccac cgcagctacc tgaacgccgt ggccgccggg ggcaactttg ccatcatcaa
     2101 ccgcctgatc ctgttccagc aactcgcgga ggccttcgag gaagtgttcg gcgcggagct
     2161 ggagttgatt tacgagatca gccacaacct ggtgcagcgc gaggagcacc cggagttcgg
     2221 cgaggtctgg gtgcaccgca agggggccac gcgcgccttc ccggcgggcc acccggcgct
     2281 ggccggcacc cgctgggcgg acaccggcca cccggtgctt atccccggca gcaaccgcga
     2341 tttcagctat atcctgcgcc cgctgccggg cgcggtgaac agcggctatt cggtgaacca
     2401 cggcgcgggg cgccgcctgt cgcggtccgc cgccaagcag aagctgtcgc aggagcaggt
     2461 caaccgcgaa taccgcgagg cgggcatcat cgtcaacatc gacggcaagg tgccgctcga
     2521 cgagtcggtg gcctgctaca agtccgccgc cgaggtggtg gacgccgtcg tccaggcggg
     2581 gctggccgag gtcgagtatc gcctgtggcc gctggcctcg ctcaagggca ctgattaacg
     2641 cgccggcgcg gtaccggcgg tgcgggccgg ttggcaaccg gcccctacat tgcggagggt
     2701 gcgcgggccg gtttcaaacc ggcccctaca gcggccatgg ggcggcgggg cggtttgaaa
     2761 ccggccccga cagcgtgggg tggggcaggg gtttgcgcgc gatggacgag cggcatgagc
     2821 atgagcaata cttcttcgac gccgccacgc tggcgcacct ggccggcgtg gtcgccgcct
     2881 acccgaatcc ctgctgcctc tgtgcgccgc gcctgggcca ggaactggag cggcggggcg
     2941 tggccgtgcg cacgctcgac atcgacacgc gcttcgccgg actggcgggc ttccgccgct
     3001 acgatgtgtt tcgtcccgaa tggctgggcg aacccttcgg cctgattctg tgcgacccgc
     3061 ccttcttccg cgtctcgctg gcgcaactgt tcacggccat ccgcctgctc agccgctacg
     3121 actaccgcca accgctgctg atcagctatc tgtcgcgccg cgccgcgaat atccggggca
     3181 ccttcgcccg cttcgacctg cgccccaccg gctactaccc cggctacctg accgtgcaaa
     3241 acgtcgagcg caacggcatc gagttcttcg gcaacctcgc gcacccgatc tagctaccgg
     3301 cgcggcctgc gaccccggcg cgccgcgctg cgtactacat ctcggagcgc gcgccgccgg
     3361 gcccgctcac gccgtcaccc aaaggaagga actgccatga cacgatcccc ctatctctcg
     3421 ccgcgcccgc cgctcgactc cactccggcc accctcaccg cgtttgagcg gttgtaccgc
     3481 gaagcggtgg cgccgggcac cggtcagccc atcgactaca ccctggccgc gccgaaatgg
     3541 cagtttctgt gctggctgtg cgagacgcag gccatcctgc tgcacggctc gggcgacccc
     3601 ggcatcgcgg agtttgagcc acgccaatcg aatgacatcg aggagttcgg caaccggcag
     3661 gcggtgtatg cagcttcgga cggcatctgg ccgatctact tcgccgtcat ggatcgcgac
     3721 cgctatgtgc ggtcgctcat gaatggctgc gtccgcgagg tggcgccgga cggcacccgc
     3781 ggcgagccgt actacttctt ctccattaac ggcgacgcgc tgccacacca accctggcgc
     3841 cagggcacaa tctacctgct gccacgggcc acgttcgagc agcagcccct gctggagaag
     3901 ggcgggagcg gttcgaggtg gcgcagtggg ccagcctggt gccggtgcgg cccctggccc
     3961 ggctggccgt cgggccggat gacttcccgt tcctatcgca gatctttgcc catgacccgg
     4021 ccctgatccg cgagcgcgcc gcccgcaacc cggatggctt cccctggctg gacgagtagg
     4081 ggaggcgccc gaccaacctc tccccccgcc cctccccgcc gcggggaggg gagtcgcttt
     4141 ctctcaggac agccatt
//
LOCUS       DASUYC010000439         2175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_33572,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000439 DASUYC010000000
VERSION     DASUYC010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33572"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="VF276_07270"
     CDS             <1..664
                     /locus_tag="VF276_07270"
                     /inference="COORDINATES: protein motif:HMM:NF022834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HEX6606696.1"
                     /translation="VTGTPAARQAAADALTRILAAQAGDEGCGRLAAHLPALLAGGDL
                     PAAIQGDPVLRGIAAGLHALHIAFETGDPDAASAALVALLGLGPGLTPSGDDVVCGIL
                     AGLAWMASTLPVNEYGAINRASTTNRLPEAARTQLIIAGLIQAVNEAAPARTNRISTA
                     LLRHAATGLLYAPAMDLSAALLAGDPVAVSEPAQRLLAIGHTSGADLAIGTLFAVCVM
                     SS"
     gene            702..>2175
                     /locus_tag="VF276_07275"
     CDS             702..>2175
                     /locus_tag="VF276_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX6606697.1"
                     /translation="MSDAEMLSIPAGQDNGSPEIVWRPSPAYIERSRLRRFMERHGME
                     SVPALVERSAREVAWFWDAVVQDLELEWFQPYTEVVDLARGIEWPRWFVGGRYNYVHD
                     AVDKHAQRLRPDAPAVAWEGEDGAVRTLTFAELYAQVNQAANALKALGIGKGDRVGVF
                     MPMVPETVVATLAISKIGAIYLPIFSGYGGAAVASRLAEGTAKLLITADGFYRAGKQV
                     PMKEAADEAAAASPTVEHVLVVRRLGRPVPWTPGRDLWWDELVPGQPAECPTEETDAE
                     DPYMIIYTSGTTGRPKGIVHTHCGFPIKAAQELAHCFDLHAGDVLFWISDIGWMMGPW
                     AIAGSLMLGATCFLYEGAINYPQPDRVWALVERHRVTHLGISPTAVRSLMGQGDHWVR
                     AHDVSSLLFLAGAGEPWNPDPWRWLFETVGGGRVPIINYSGGTEVSGGILSCNPLLPL
                     KPCAFSTVVPGMAADVVDAEGRPVRGEVGELVIRQPWAGST"
BASE COUNT          326 a          738 c          775 g          336 t
ORIGIN      
        1 cgtaaccggc accccggcgg cccggcaggc ggcagcggac gcgctgaccc gtatcctcgc
       61 ggcccaggcc ggggatgagg gttgcgggcg cctcgccgcc catctgccgg ccctactcgc
      121 cgggggtgac ctacccgccg cgatccaagg cgacccggtg ctgcggggca tcgcggcggg
      181 gctacacgcg ctacatatag catttgaaac aggcgatccg gatgcggcat ccgccgccct
      241 ggtggctctc ctgggactcg gtcccggcct gaccccttcc ggcgacgacg tggtctgcgg
      301 gatcctggcg ggcctcgctt ggatggcgag tacgctgccg gtaaacgagt acggcgcgat
      361 aaatcgcgca tctacaacca ataggttgcc ggaagccgca cgcactcaac tgataatcgc
      421 cggtctaatc caggcggtga acgaggccgc cccggcgcgc acgaaccgca tcagcaccgc
      481 cctgctgcgg cacgccgcca ccggcctgct ctacgccccg gctatggatc tcagcgccgc
      541 cctgctggcc ggcgatccgg tagccgttag cgagccggcc cagcgcttgc tcgccatcgg
      601 gcacacgtcg ggcgcggatc tcgcgatcgg tactttgttc gcagtttgtg ttatgtcgag
      661 ttaagaggct gggtttttac ccctcaagga gaatcacccc catgtccgat gccgaaatgc
      721 tctccatccc cgccggccag gacaacggct cgccggagat agtatggcgg cccagcccgg
      781 cctatatcga gcgcagccgc ctgcgccgct tcatggagcg ccatgggatg gagtccgtgc
      841 ccgcgctggt cgagcggtcg gcgcgcgagg tggcctggtt ctgggatgcg gtggtgcagg
      901 acctggagtt ggagtggttc cagccgtaca ccgaagtcgt ggatctggcg cgcgggatcg
      961 agtggccgcg ctggttcgtg ggcgggcgct acaactacgt gcatgacgcc gtggataagc
     1021 acgcgcagcg cctgcggccc gatgcccccg ccgtggcctg ggagggcgag gacggcgccg
     1081 tgcgcacact gaccttcgcc gagctatacg cgcaggtaaa ccaggcggcc aacgcgctca
     1141 aagcgctggg catcggcaag ggcgaccgcg tgggcgtgtt catgcccatg gtgccggaga
     1201 cggtggtggc gaccctggcg atctccaaaa tcggcgcgat ctacctgcct atcttctccg
     1261 gctacggggg cgcggccgtc gcttcgcggc tggccgaggg cacggcgaag ctgttgatca
     1321 cggcggacgg cttctaccgc gcgggcaagc aggtgccgat gaaggaggcc gccgacgaag
     1381 ccgccgcggc cagccccacg gtcgagcatg tgctggtggt gcgccgcctg ggacgcccgg
     1441 tgccctggac gccagggcgt gacctctggt gggatgagct ggtgccgggc cagcccgcag
     1501 agtgccccac cgaagagacg gacgccgaag acccctacat gatcatctac acctccggca
     1561 ccaccgggcg gcccaagggc atcgtccaca cccactgcgg cttcccgatc aaggcggcgc
     1621 aggaactggc gcactgcttc gatctgcacg cgggcgacgt gctgttctgg atcagcgaca
     1681 tcggctggat gatggggccg tgggccatcg ccgggtcgct gatgctgggc gcgacctgct
     1741 tcctctacga aggggcgatt aactatcccc agccggaccg ggtctgggcg ctggtcgagc
     1801 gccaccgggt gacgcacctg ggcatctcgc ccacggcggt gcgctcgctc atgggccagg
     1861 gcgaccactg ggtgcgggcg cacgacgtaa gcagcctgct gttcctggcg ggcgcgggcg
     1921 agccgtggaa ccccgacccc tggcgctggc tgttcgagac ggtgggcggc gggcgggtgc
     1981 cgatcatcaa ctactcgggc ggcacggagg tctccggcgg catcctgagt tgcaatccgc
     2041 tgctgccgct caagccctgc gccttcagca ccgtcgtgcc gggcatggcc gccgacgtgg
     2101 tcgacgcgga gggccggccc gtgcgcggcg aggtgggcga actggtcatc cgccagccgt
     2161 gggcgggcag cacgc
//
LOCUS       DASUYC010000440         4182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_21750,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000440 DASUYC010000000
VERSION     DASUYC010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21750"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VF276_07280"
     CDS             <1..387
                     /locus_tag="VF276_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEX6606698.1"
                     /translation="EDGRFYFLETAARVGGANIAELVEAATGINLWAEWAKIELAGGE
                     RPYELPPTREDYAGLIISLARQDYPDTSAYQDPEVVWRMVRLHHAGLIVAAPDPARVQ
                     VLLDDYSRRFYQDFWATLPAPDKAAH"
     gene            complement(596..2188)
                     /locus_tag="VF276_07285"
     CDS             complement(596..2188)
                     /locus_tag="VF276_07285"
                     /inference="COORDINATES: protein motif:HMM:NF018332.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEX6606699.1"
                     /translation="MATVETNSPPAPLAAAPHPQTHKGLIIFAVMLTTALSALDANIV
                     GTAIPSIVGALRGIGLLPWLVTAFMLTSTVSVPLYGKLADLYGRKPVLLAGVTLFLAG
                     SALCGVAGSMEQLIAFRALQGLGAGSVVPVTLTLIGDLFEMEERARLQGLFSAVWGVS
                     SVLGPLVGGFIVTVWSWPWVFLVNVPVGFLAMAILALYLREPVRSHARRPSLDLLGAA
                     TLTGGLTAALTALSLVSNGAGWGSPAVLGLLGLALALLVAFVVVERRAAEPLIPLDLL
                     TAPITRVSIAAGLFSSGLVFGGGAYLPILVQGVWQGSPLEAGLALAPLSIGWPLASTV
                     AGRAILRWGYRPVAVIGAVLIALGGLLLLPLGPGGPIWLLPVSMFVQGLGCGFNFTTL
                     LMAVQNAASWERRGAATSLYQFSRNIGGVIAVAVLGFALSTTLAGALAQIPALPGVPA
                     AGAGGSQLGPASVLLDLNARTHLAPATLAALEDALAAGLRPVLWIAAFLGLGTALATW
                     FFPAERIPTRAAPAEEPALPGA"
     gene            complement(2442..3023)
                     /locus_tag="VF276_07290"
     CDS             complement(2442..3023)
                     /locus_tag="VF276_07290"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX6606700.1"
                     /translation="MQAPSTILPHLGPPAEVMPVHWGRLATALGPVYAAATPEGVVSI
                     TTSVGSDDRFVAELEGDLGPRAHLIAGASPILATALAELAGYFAGETRTFTVPLDLRL
                     LRGAFNHRVLAELRTVPWGHLVSYSELARRVGAPRAARAVGSACKHNPLALVIPCHRV
                     VHANGGIGGYGAPGIAYKRALLAIEGIVFPVTS"
     gene            3140..4015
                     /locus_tag="VF276_07295"
     CDS             3140..4015
                     /locus_tag="VF276_07295"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6606701.1"
                     /translation="MAPTAQDHINIGMQLVHDGDTNAALEQFNHALQLAPGAPAALLG
                     RGFLRHELGNLEGALQDLTALLAQQPDHLGARLRRGLVLRELGRVEQAGADFAYAVQR
                     APRDPDVLYNRALFYQEIGDMPAALADLTRALDLRPDHIPTLNNRGLARAVLEDVPGA
                     LADFNRSLALQADQPRVLNNRGLARRTIGDLQGAMDDFTRALALDSGRPDTLYNLGVT
                     RVRAGNPLGAVESFQRALAFWPDDPELTYRVGWAYAHAGRCREALEWLGEAISLDPQL
                     GDLAAEDPAFDPCRG"
BASE COUNT          697 a         1560 c         1397 g          528 t
ORIGIN      
        1 gaggacgggc gcttctactt cctggagacg gcggcccgcg tgggcggggc caacatcgcc
       61 gagctggtcg aggcggccac gggcatcaac ctctgggccg agtgggccaa gatcgagctg
      121 gccggcggcg aacgccccta tgagttgcct cccacccgcg aagactacgc cgggctgatc
      181 atctcgctgg cccgccagga ctaccccgat accagcgcct accaggaccc ggaggtcgtc
      241 tggcgcatgg tgcgcctgca ccacgccggg ctcatcgtcg ccgcgcccga cccggcccgc
      301 gtccaggtcc tgctcgacga ctacagccgc cgcttctacc aggacttctg ggccactctg
      361 cccgcccccg acaaagcggc gcactaacca tcccgcccgc tattattcac cgcgaagacg
      421 cgaaggacgc gaagaacggg atcaatataa aaatcttcgc gtccttcgcg ccttcgcggt
      481 gaattatcga atcttcccga agacggcgca aaaacgcgaa ggatgcgcac aacaagatcc
      541 ataaaaatct tcgcgtcctt cgcgtcttca cggtgaaaaa ccgtacgagg cgagcctaag
      601 cgccgggcag ggccggttct tcggcgggcg cggcgcgcgt ggggatgcgc tcggcgggga
      661 agaaccaggt cgccagcgcc gtgcccaggc ccaggaaggc ggcgatccac agcaccgggc
      721 gcaggccggc ggccagcgcg tcttcgagcg cggccagcgt ggcgggcgcc aggtgggtgc
      781 gcgcgttcag gtcgagcagc acgctggccg gccccagttg cgagccgccc gcccccgccg
      841 cgggcacgcc gggcagggcc gggatctgcg ccagcgcccc ggccagcgtc gtgctcagcg
      901 cgaagcccag cacggccacg gcgatcacgc cgccgatgtt gcgcgagaac tggtagaggc
      961 tggtcgccgc gccccggcgc tcccagctag ccgcgttttg caccgccatc agcagggtgg
     1021 tgaagttgaa gccgcagccc aggccctgca cgaacatcga caccggcaag agccagatcg
     1081 gcccgcccgg ccccagcggc agcaacagca gcccgcccag cgcgatcagc accgcgccga
     1141 tcaccgccac gggccggtag ccccagcgca ggatggcccg cccggccacc gtgctcgcca
     1201 gcggccagcc gatcgacagc ggggccagcg ccagcccggc ctccagcggc gacccctgcc
     1261 acacgccctg caccaggatg ggcaggtagg ccccgccgcc gaagaccagc cccgacgaga
     1321 acagcccagc cgcgatggac acccgcgtga tcggcgcggt cagcaggtcg agcgggatca
     1381 gcggctcggc ggcgcggcgc tccacgacga cgaaggccac cagcagcgcc agggccagcc
     1441 ccagcagccc cagcacggca gggctgcccc agcccgcgcc gttgctcacc aggctgagcg
     1501 ccgtcagcgc cgcggtcagc ccgccggtca gcgtggccgc gcccagcagg tcgagcgagg
     1561 gccgccgggc atggctgcgc acgggttcgc gcaggtagag cgcgaggatc gccatcgcca
     1621 ggaagcccac cggcacgttg accaggaaca cccagggcca gctccacacg gtgacgatga
     1681 acccgcccac cagcggtccc agcacgctcg acacgcccca gacggcgctg aacagccctt
     1741 gcagccgcgc ccgctcctcc atctcgaaca ggtcgccgat gagggtcagc gtcaccggca
     1801 ccacgctgcc cgcgcccagc ccctgcaacg cccggaaggc gatcagttgc tccatcgacc
     1861 cggccacgcc gcacagcgcc gacccggcca ggaagagcgt caccccggcc agcagcaccg
     1921 gcttgcgccc gtagaggtcg gccagtttgc cgtacagcgg cacgctcacg gtcgaggtaa
     1981 gcatgaacgc cgtgaccagc cagggcagca ggccgatccc gcgcaacgcc cccacgatgc
     2041 tcggaatggc cgtgcccacg atgttggcgt ccagcgccga cagcgccgtg gtcagcatga
     2101 cggcgaagat gatcaggccc ttatgggttt gcgggtgcgg cgccgcggcc aggggcgcgg
     2161 gcggcgaatt ggtttcgacg gtagccatga tgtgcgcata cctctacgat aaaatccatg
     2221 aatgcgatgg acggctgccc tgatataagc atgaggcggg ccgcgcgggg cgccgcccgg
     2281 aggcgcaagc ctccggctac cgctacgaag gccgcctgcg cggcccgatc ccttagcccg
     2341 cgcaggcggg cttcgtaaac gttgcccgcc cgttcacggg ccgggcgatc ccacgttata
     2401 ggaaacgcgc catcccggtg ggctggatcg cccggcgcgg gtcaggatgt gaccgggaac
     2461 acgataccct cgatcgccag cagcgcgcgc ttgtaggcga tgcccggcgc gccgtagccg
     2521 ccgatgccgc cgttggcgtg gaccacccgg tggcagggga tgaccagcgc cagcggattg
     2581 tgcttgcagg cgctgcccac ggcgcgggcg gcgcgcggcg cgcccacccg gcgggccagt
     2641 tcgctgtagc tgaccaggtg gccccagggc acggtgcgca actcggccag cacgcggtgg
     2701 ttgaacgccc cgcgcagcag ccgcagatcc agcggcacgg tgaaggtgcg cgtctccccg
     2761 gcgaaatagc ccgccagttc ggccagcgcc gtggcgagga tggggctggc cccggcgatc
     2821 aggtgggcgc gcgggccgag gtcgccctcc agttcggcca cgaaccggtc atcgctgccc
     2881 acgctggtgg tgatgctgac cacgccctcc ggcgtggccg cggcatacac cgggccgagc
     2941 gccgtcgcca ggcggcccca gtggaccggc atgacctcgg cgggcggccc caggtggggc
     3001 aatatcgtgc tgggtgcctg cataatggtg cctttcttgc tccccagggt acggatcttg
     3061 tggtattata gcacacgcca tccagaggtc caaccagagt gtaccggccg tttacgattc
     3121 ccgcgcccgg aggtgacgca tggcgcccac tgcccaggat catatcaaca tcgggatgca
     3181 actggtccac gacggcgaca cgaacgccgc gttagagcag ttcaaccatg ccctgcaact
     3241 ggcgccgggg gcgccggccg ccctgctggg ccgcggcttc ttgcgccacg aactcggcaa
     3301 cctggagggc gcgttgcaag acctgaccgc gctcctggcc cagcagcccg atcacctggg
     3361 ggcgcgcctg cggcgcgggc tggtgctgcg cgagctgggc cgcgtcgagc aggcgggcgc
     3421 tgatttcgcc tatgccgtgc agcgcgcccc gcgcgacccc gatgtgctct acaaccgcgc
     3481 cctgttctac caggagatcg gcgatatgcc cgccgctctg gccgacctga cccgcgcact
     3541 cgacctgcgc cccgaccata tccccacgct gaacaaccgc ggcctggctc gcgccgtgct
     3601 cgaagacgtg ccgggcgccc tggccgactt caaccgctcg ctggcgctgc aagccgacca
     3661 gccgcgcgtg ctcaacaacc gcggcctggc ccgccgcacc atcggcgacc tccagggcgc
     3721 catggacgac ttcacccgcg ccctggccct cgattcgggc cgccccgaca cgctctacaa
     3781 cctgggcgtc acccgcgtgc gcgccggcaa cccgctcggc gccgtggagt ccttccagcg
     3841 cgccctcgcc ttctggcccg acgatcccga actgacctac cgcgtcggct gggcctacgc
     3901 ccacgcgggc cgctgccgcg aagccctcga atggctcggc gaagccatct cgctcgaccc
     3961 ccagctcggc gacctggccg cggaggaccc ggcgttcgat ccgtgccggg gatagccgtc
     4021 agccgtcagc cgtcagctat caggagacga ggatacgaga gcttacggcg gattgctgac
     4081 cgctaacagg agtacagtac gatcaaatac caactgacgg ccgaccgctg acggctgacg
     4141 gctgacggct gacggctgac cgttaacagg agcacgatgc gc
//
LOCUS       DASUYC010000441         4315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1616226, whole genome shotgun sequence.
ACCESSION   DASUYC010000441 DASUYC010000000
VERSION     DASUYC010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1616226"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1289
                     /locus_tag="VF276_07300"
     CDS             <1..1289
                     /locus_tag="VF276_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606702.1"
                     /translation="PMPAAAQDNSHTFPETGKTVSGRFWTYWQAHGGLAQQGYPISAE
                     MPEQSDLNGQTYTVQYFERAVFEYHPENQPPYDVLLSQLGTYRYKAKYGAAGAPGQRA
                     STDNPRVFAETGHSVGGKFRTYWETHGGLAQQGYPISDEFQEVSDLNGQAYTVQYFER
                     AVFELHPENAPPYDVLLSQLGTFQYKQKYLQPAASPTPAASPTPVAPNPCGSDVPAGQ
                     DATIAPGCGPIGTAFHIHLRGFSAGESIDFSLVGPGHQKLLIFPPEAGTAGATGEADV
                     VFDSTQLNDAAPPHEGVWTITYHGATSNHPATASFKITPAAGSPPGGSADCSGIPASQ
                     NQEVTPACGAAGTRFEFSGSGFKPGELVGIYATMPSGAVFGAPFQRRADAAGRVTGLT
                     LTTSAATPPGIWANTMEGTETHAKSIGYFKVLPPVP"
     gene            1528..2403
                     /locus_tag="VF276_07305"
     CDS             1528..2403
                     /locus_tag="VF276_07305"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6606703.1"
                     /translation="MRLALLVIGFAALGYLGIAAYAADRFSHPDRLPVGRCMDYHVVC
                     EDVQFTSTGDNVPLRGWFIHTAGIRTILMLHGRNGRRDDPAIGMMDIAQAFVHHGYNV
                     LLFDFRAHGMSGGDRYSLGDWERRDIAGALLFLHSRGVTAVGAIGFSMGAGTLLRATP
                     DHPELRAVVLDSPFADLTPLIELHLTEVSGLPRFFAPGIIGMAQVLFGMDLVDNRPAE
                     EMARLGDRPVLLIHSTTDEYIPISQAYTLQRAGARNPHLQAWFPAGPAHARAFKENPT
                     EYLRRVLGFFDENLP"
     gene            2822..3337
                     /locus_tag="VF276_07310"
     CDS             2822..3337
                     /locus_tag="VF276_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606704.1"
                     /translation="MDTTVTAAGQIATQTFDIDVEKQKLRLEARGPGEQVNIIVIGND
                     SWTSTDGGKTYRADPASESKAEGFGPLIDEWRIGDMLGVATAPSAIKAGMPPMEAIDG
                     VETNHYVIDNKDFAGVGLGTAGTMEFWITPRSNPTVRQERMTILVGGAATISTTRWSK
                     FNEDFAITAPQ"
BASE COUNT          744 a         1493 c         1341 g          737 t
ORIGIN      
        1 cacccatgcc ggccgctgca caggacaaca gccacacctt ccccgaaacc ggcaagacag
       61 tcagcgggcg tttctggacc tactggcaag cgcatggcgg gctagcccag cagggctatc
      121 cgatttccgc tgaaatgccg gagcagagcg acctgaacgg gcaaacctac acggtgcagt
      181 acttcgagcg cgccgtcttc gagtatcacc cggaaaacca gccgccgtac gacgtgctgc
      241 tcagccaact cggcacgtac cgctacaagg ccaagtacgg cgccgcgggc gcccccggcc
      301 agcgggccag caccgacaat ccgcgcgtgt tcgccgagac tggccatagt gtgggcggga
      361 agtttcgcac ctactgggaa acccatggcg ggttggcgca gcagggctac ccgatctccg
      421 atgagttcca ggaagtgagc gacctcaacg gtcaggccta tacggtgcag tattttgagc
      481 gggcggtctt cgaattacac cccgaaaatg cgccgcccta cgatgtgctg ctcagccaac
      541 ttggcacctt ccaatacaaa cagaaatatc tgcagcccgc ggcgagcccc accccggccg
      601 ccagtcccac accggtcgcg cccaacccct gcggcagcga tgtgcctgct gggcaggatg
      661 cgacgatcgc ccccggctgc ggtccgattg gcaccgcgtt ccacatccac ctgcgcgggt
      721 tcagcgcggg cgaatccatc gacttctcgc tggtcggacc cggccaccag aagttgctta
      781 tcttcccgcc ggaggccggg acggccgggg ccacgggcga agcggatgtg gtgttcgaca
      841 gcacccaact caacgatgcc gcgccgcccc acgagggcgt ctggaccatc acctatcacg
      901 gagcgacctc taatcacccg gccacggctt ccttcaagat cactccagcc gcgggctcgc
      961 cgcctggtgg gagtgccgac tgctcaggga tcccggccag tcagaatcag gaggtcactc
     1021 cggcatgcgg cgcggcgggc acccgctttg agttcagcgg gtcgggcttt aagcccggcg
     1081 aactcgtcgg gatctatgct acgatgccgt ccggagccgt gtttggcgcc ccgttccagc
     1141 gccgcgccga tgccgcgggc cgggtgaccg ggctaacctt aaccaccagt gccgcgaccc
     1201 cgcccggcat ctgggccaac acgatggaag gcaccgagac ccatgcaaag tcgatcggct
     1261 actttaaggt cttgccgccg gtgccctaaa gtccaccagg cgggcgttac gccccacgcg
     1321 cgatcgctgc gcgctgatta atagcctgaa gtcgcccttg tagccacgca cactcatctt
     1381 ccgcattggc agtggggagt atcgctggta tggcgatact ccccggatat cggcccggat
     1441 gcacgaaatc gttagcgcta cttgtggagg caacaaatga ctatttccat ccccaccggg
     1501 atcacggccg ctctgggccg attcctattg cggctggcgc tgctggtaat cggcttcgct
     1561 gccctgggct acctcggtat tgcggcctac gccgcggatc gcttctcgca tcccgatcgg
     1621 ctccccgtgg gccggtgcat ggactaccat gtggtctgcg aggacgtgca gtttacgagc
     1681 acgggagata acgtgcccct ccgcggctgg ttcatccata ccgcgggcat ccgcacgatc
     1741 ctgatgctcc atgggcgcaa cggccggcgc gacgatccgg cgattggtat gatggacatc
     1801 gcccaggcgt tcgtgcacca cggctacaac gtcctgctct ttgattttcg agcccatggc
     1861 atgtcgggcg gggatcgcta ctccctgggc gattgggaac ggcgggatat tgccggcgct
     1921 ctgctctttt tacacagccg cggcgtcacg gccgtcggcg cgattggttt ctccatgggc
     1981 gccggcaccc tcctgcgggc gaccccggat cacccggaac tccgcgccgt cgtgctggat
     2041 tctccctttg ccgatctgac gccgctgatt gagttgcatc tgaccgaggt cagcgggttg
     2101 ccccgcttct ttgcgcccgg tattatcggc atggcccagg tgctgttcgg catggatctg
     2161 gtggacaacc gccccgcaga ggagatggcg cggctgggcg atcgcccggt cttgctcatt
     2221 cacagtacga ccgacgagta cattcccatc agccaggcct acacgctgca gcgggccggc
     2281 gccaggaatc cccacttgca agcctggttt cctgccggcc ccgcccatgc tcgggcgttc
     2341 aaggagaatc ccacggaata cttacggcgc gtattggggt tctttgacga gaacttgccg
     2401 taacagcgct ttgtctactg atgaggtagg gaggaaagcg atgggtgcat cacataaact
     2461 ccgtgcgatc aaccggatac tcatagtgct gctgaacctc ggcttggccg cctgcagcgc
     2521 ggcggctccg tcccccatct ccgaagcgcc gccgggccgc gccaccccga ctgtcgcgcc
     2581 ccagggactt cccaccgtgg cgcgcgcact cgcgacctca gcgataatgc tatccggcac
     2641 cccggccccg atgccgccaa ccaccgttcc tgtcgccacc gctgattcgg ttcctactcc
     2701 cctaccaaca cacgccacag cggacgcgac cgctactccg ggccctgcca gtgtgcccac
     2761 cggcgccggc aatgccctcc ggcaagcggc cgccaatatg cgcgcgctga agagcttcca
     2821 catggacacc accgtcacgg ccgctgggca gatcgcgacc cagacattcg atattgacgt
     2881 ggaaaaacag aagttgcggc tcgaagcccg cggccctggc gagcaggtga atatcatcgt
     2941 cattggcaac gactcctgga ctagcaccga cggcggcaag acgtaccggg ccgatccggc
     3001 cagtgagagc aaggcggagg gattcggacc tttgattgac gaatggcgga tcggcgatat
     3061 gctgggcgtg gccacagcgc ccagcgcgat taaagcgggc atgccaccca tggaggctat
     3121 cgatggtgtc gagacgaacc actatgtcat cgacaacaaa gatttcgctg gggttggcct
     3181 gggtacagcg ggtaccatgg aattctggat cacgccccga tcgaatccga cggtacgcca
     3241 ggaacggatg accattctgg tcggcggtgc cgcgacaatc agcaccacgc gctggagcaa
     3301 gttcaacgaa gacttcgcca tcaccgctcc tcagtgatcg cgtgcctaca gcgtatctag
     3361 cgtccatgta acgcgaggaa ccggcggggg gtggcagcgc gcgccggcct tgccgcagat
     3421 cggggtgacc cacccaggtc gcgatgcgca cgcctgtaga gcgcgccttt ctcgccccgc
     3481 gcaggcaggg cgtgatctgc ctggcgctgc cggagatcac ccgcgtgata agcggctacc
     3541 ggctcgccta gcggctcgac gcggccggcg attacccgcg ccacgtcgtg gtctgcctcc
     3601 gccggctgat cgatcttagt ctgcccccgc gccaggcgcg cgcctgatcg ccgcggcacc
     3661 gtgggccagg caggagatcg cccgcgcgcc ggatcggcac cggcgcgcag accgcgcccg
     3721 cttcctgtac cacctggccg tcattcacga ggcgcgcgac gaccgggctg ggtataccag
     3781 actgatgcac ctacggcaga tccctgtgcc gggcgcgccg gattccatcc ggcagcaagc
     3841 ctggcgcgtc ttgggcgacc tctctgagtt ttgcgccgac cccatcgacg ccctgttgac
     3901 agccttccgc ccacggatgc cctgccgcag ccggttctgg aatgcgccgg aggcgggctt
     3961 ggcgcctttg atctgagcgc agggccggag gtgatcacgc ggacttgggt gtgggtgcgg
     4021 gactgagcct gcgggccgcc gcccttcccg gctaccaccc tcccgcccgc tcccgatccg
     4081 ccggggaatg gggcgatacg ctggacctgt cgcggccggt ccaccggggc gccgcgcggc
     4141 gccccggtgg agaggggggc acggccaaag tgggaaggcg gaacgcggaa cgcggaacag
     4201 gaattccgcc ttgcgacctt cgatttccca cttcggcagt cctgcctccc ggccccctgt
     4261 gaagggagca tgcccgatgc ccccgcccgc ccattccgac ttccgcgctc cgcgc
//
LOCUS       DASUYC010000442         3844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1476021, whole genome shotgun sequence.
ACCESSION   DASUYC010000442 DASUYC010000000
VERSION     DASUYC010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1476021"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VF276_07315"
     CDS             complement(<1..485)
                     /locus_tag="VF276_07315"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX6606705.1"
                     /translation="MTRILTRPDLESLLDPPAVIAALRAAFAALGAGAAQVPQRMGLE
                     LPTGSSFFYMPGFLDAAPAPVLGTKVVAIQPDNPARGLPRIQAVCLLHDPVTGDLRAV
                     LDATFLTEIRTACTSALVTDYLALPGADTLLVFGTGPQARAHLDAIAAVRPLRAAWVV
                     G"
     gene            559..1365
                     /locus_tag="VF276_07320"
     CDS             559..1365
                     /locus_tag="VF276_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606706.1"
                     /translation="MDTAVGLVQAYLHLNGYLVLTEVPVQLEANGAFYTETDLDILAV
                     RFPHEPEAHPSRSSTWEEFYRGVDPALDLDTNSIDFIVGEVKEGRSHANPALRRQATL
                     HFALHRIGCCPEGDVMKVAAEVARKGEAHLQVRGGSPTRVRLVVFGGRGDLHDEGLTE
                     IPLHQVALYVQERLQQYRAVLQGIEFKDETLSLFRLIDKIGLELAINTHQTAIPLPET
                     AARRPAPPHAPGSEQRTAAGRTPAARTISTSPPRRPRKQPWYRRPFRRSR"
     gene            complement(1514..>3844)
                     /gene="mutS"
                     /locus_tag="VF276_07325"
     CDS             complement(1514..>3844)
                     /gene="mutS"
                     /locus_tag="VF276_07325"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX6606707.1"
                     /translation="SRSDMKAWAAEVRSDESDSDTAPDAARPRRAHHTERAAQNWRVA
                     GHITPTSPREWSLDKARATLMGQFHVASLDGFGVAKWPLAVRAAGAILAYLQATHSTA
                     LQQLTHLSSYSTEAYMALDPQTRRNLELVAGPSGGLQFSLLSVIDQTLTPMGGRLLSR
                     WLNQPLVDLPRLHARQDAVQLLYSDEPLRGAIRTQLKGMPDLERLTNRVLQGIAGPRD
                     LNAIRNALTALPGVRDAFESSGEWRPAPAPDRGPMIMESNGRGPAVAAPPVAKEAPAA
                     GPSTFHSLLTRLQPCQDVVDVITQAIAEDPPAVLGTGDAIRPGFRPELDQLREASRNA
                     QGWVANLEEAERNRTGIPSLKVGYNKIFGYYLEVSNAHKDRVPANYIRRQTLVGAERY
                     ITPELKEAESLILNAKERLVELERSLFQEVLATVAACAERLLGAAQALAHIDVFAGLA
                     EVARTRNYVRPDLLAEGPITIAQGRHPVVERSLRDEIFVPNDVHLDNAASQIIILTGP
                     NMAGKSVYLKSIAQIILLAQIGAFVPADSARLGLVDRIFTRVGAQDDIATGQSTFMVE
                     MVETANILNHATPRSLVILDEIGRGTSTYDGLAIARAVVEYIHNHPRLASKTLFATHY
                     HELTDLERVLPRVRNYNVAVAEEGEELVFLRKIVPGGADRSYGIHVARLAGVPKSILK
                     RAEDILRELEQGGSKERRRAVMGDGMGLPALPANGQLSFLAGNAPAPGAAPAPVPALP
                     DPVVEELRELKVDDMTPLEAMGKLYELVRKARDSAD"
BASE COUNT          575 a         1278 c         1401 g          590 t
ORIGIN      
        1 tggcccacga cccaggctgc gcgcagcgga cgcacggcgg cgatggcgtc gagatgagcg
       61 cgcgcctgcg ggccggtgcc gaagaccagc agcgtgtccg cgccgggcag tgccaggtaa
      121 tcggtcacca gggccgaggt gcaggccgtg cggatctcgg tcaggaaggt cgcgtcgagc
      181 accgcccgca ggtcgccggt cacggggtca tgcagcaggc agacggcctg gatgcgcggc
      241 agcccgcgcg ccgggttgtc gggctggatg gcgaccacct tggtgcccag caccggcgcc
      301 ggggcggcgt cgaggaagcc cggcatatag aagaaggagc tgccggtagg gagttccaac
      361 cccatgcgct gcggcacctg ggcggccccg gcccccagcg cggcaaacgc ggcgcgcagc
      421 gcggcgatga ccgcgggtgg gtcgagcagc gattccaggt cagggcgggt caggatgcgg
      481 gtcataaggc gaacctccgg cacagcgaac gtgcccgtcc gttagatagg tatacgcagc
      541 gacgaaggga gaacagcgat ggataccgcg gtcggcctgg tgcaagccta tctccacctc
      601 aacggctacc tggtgctcac cgaggtgccg gtccagttgg aagcgaacgg tgccttctac
      661 acggaaacgg acctggacat cctggcggtg cgcttcccgc atgagccgga ggcgcacccc
      721 agccggtcca gcacctggga agagttttac cggggcgtgg acccggcgct ggacctggac
      781 acgaacagca tcgacttcat cgtcggcgag gtgaaggagg gccgcagcca cgccaatccg
      841 gcgctgcgcc ggcaggccac gctccacttt gccctgcatc gcatcggctg ctgcccggaa
      901 ggcgacgtga tgaaggtcgc cgccgaagtg gcccgcaagg gcgaggccca tctccaggtg
      961 cgcggcggca gccccacccg cgtgcgcctg gtggtgttcg gcgggcgcgg cgacctgcac
     1021 gacgagggcc tgaccgagat cccgctacac caagtggcgc tctacgtgca ggaacgcttg
     1081 cagcaatacc gcgcggtgct acagggcatc gagttcaagg acgagacgct gagcctcttc
     1141 cgcttgatcg acaagatcgg gctggaactg gcgatcaaca cacaccagac ggccatcccc
     1201 ctgccggaga ctgccgcccg ccggccggcg ccgccccacg cgcccggttc cgagcaacgc
     1261 actgcggcag gacgcacccc ggcggcccgc accataagca catcgccacc ccggcgcccg
     1321 cgcaagcagc cctggtaccg gcgcccgttc cgccgctccc ggtagggtgg cagccccgcg
     1381 cggcgggtgg cgctactgcg tagcccgtca ccgcgtgctt gtcactttga gcgcagcgaa
     1441 gagcctcgtc ttttcccccc aacccgacgg agattcttcg ctccgctcag aatgacgaga
     1501 ccggcgggcg gcgctagtcg gcgctgtcgc gggctttgcg gaccagttcg tagagcttgc
     1561 ccatggcttc cagcggcgtc atatcgtcca ccttcagttc gcgcaactct tcgaccacgg
     1621 gatcgggtag ggcgggcacc ggcgcggggg cggcgccggg ggccggggcg ttgccggcca
     1681 ggaacgatag ctggccgttc gcaggcaacg cgggcaggcc catgccgtcg cccatcaccg
     1741 cgcggcggcg ctccttcgac ccgccctgct ccaactcgcg caagatgtcc tcggcccgct
     1801 tgaggatgct cttgggcacc ccggccagcc gggcgacgtg gatgccgtag gagcgatcgg
     1861 caccgccggg cacgatcttg cgcaggaaaa ccagttcctc gccttcttcg gccacggcca
     1921 cgttgtagtt gcgcacgcgc ggcagcaccc gctccaggtc ggtcagttcg tggtagtgcg
     1981 tggcgaacag cgttttggag gccaggcgcg ggtggttgtg gatgtactcg accacggcgc
     2041 gggcaatcgc cagcccgtca taggtcgagg tgccgcgccc gatctcgtcc aggataacca
     2101 gcgagcgcgg cgtggcgtgg ttcaggatgt tggccgtctc gaccatctcg accatgaagg
     2161 tggactggcc ggtggcgatg tcgtcctgcg cgccgacgcg ggtgaagatg cggtccacca
     2221 ggcccaggcg ggcgctgtcg gcgggcacga aggcgccgat ctgggccagc agaatgatct
     2281 gggcgatgct cttgaggtag acactcttgc cggccatgtt cggaccggtc aggatgatga
     2341 tctggctggc ggcgttgtcg agatgcacgt cgttgggcac gaagatctcg tcgcgcaggc
     2401 tgcgttcgac caccgggtgg cgaccctgcg cgatggtgat cggcccctcg gccagcaggt
     2461 cggggcgcac atagttgcgg gtgcgcgcca cctcggccag cccggcgaac acgtcgatat
     2521 gggccagcgc ctgggccgcg cccagcagcc gctcggcgca cgcggccact gtcgccagca
     2581 cttcctggaa cagcgaccgc tccaactcca ctagccgctc cttggcgttg aggatgagcg
     2641 actcggcctc cttcaactcg ggcgtgatgt agcgctcggc gccgaccagc gtctggcggc
     2701 ggatatagtt ggccgggacg cgatccttgt gcgcgttgct gacctccagg tagtagccga
     2761 agatcttgtt gtagccgact ttgagcgagg ggatgccggt gcggttgcgc tccgcttcct
     2821 ccaggttggc aacccagccc tgggcgttgc ggctggcctc gcgcaactgg tcgagttcgg
     2881 gccggaagcc gggccggatg gcgtcgccgg tgcccagcac agcgggaggg tcttcggcga
     2941 tggcctgggt gatcacgtcg accacgtcct ggcagggttg caggcgcgtg agcagcgagt
     3001 ggaacgtgct cggcccggcg gcgggtgcct ctttcgcgac aggcggggcg gccacggcgg
     3061 gaccgcgccc gttgctctcc ataatcatcg gcccgcggtc cggcgcgggc gccgggcgcc
     3121 actctccgct cgactcgaag gcgtcgcgca cgccgggcag cgcagtcagc gcgttgcgga
     3181 tggcgttgag gtcgcgcggc ccggcgatgc cttgcagcac gcggttggtg aggcgctcca
     3241 ggtcgggcat acccttaagc tgggtgcgga tcgcgccgcg caacggctcg tcggagtaaa
     3301 gcaactggac ggcgtcctgg cgggcatgga gccgtggcag atcgaccagc ggctggttga
     3361 gccagcgcga cagcaggcgc ccgcccatcg gcgtcagcgt ctggtcgatg accgagagca
     3421 gcgagaattg caggccgccg ctgggcccgg ccaccagttc gaggttgcgc cgggtctgcg
     3481 ggtcgagggc catgtacgcc tcggtgctgt agctgctcag gtgggtcagt tgctgtagcg
     3541 ccgtgctgtg cgtggcctgg agataggcca ggatggcccc ggcggcgcgc acggccagcg
     3601 gccatttggc gacgccgaag ccgtcgagcg aggcgacgtg gaactggccc atcagcgtgg
     3661 cgcgcgcttt gtccaggctc cattcgcgcg gactggtggg cgtaatatgc ccggcgacgc
     3721 gccagttttg cgccgcgcgc tcggtgtggt gggcgcggcg cgggcgggcg gcatccggcg
     3781 cggtgtcgct gtcggattcg tcgctgcgca cctcggcggc ccaggctttc atatccgacc
     3841 ggct
//
LOCUS       DASUYC010000443         2018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_6072,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000443 DASUYC010000000
VERSION     DASUYC010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6072"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1736
                     /locus_tag="VF276_07330"
     CDS             <1..1736
                     /locus_tag="VF276_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606708.1"
                     /translation="VLGVLLVLGVVSFAAGQAWDQQAKVTHYQAGSRAVNALDWARAA
                     VEYGAAGAYRDAPAQAAQAASFLAERDRLYAAAQTAARRYDWPAVLDLLPRLRQLGPD
                     YQETRHLTDVVAALAPGLALSGTVALRAGNGDPGLYSYLPDGWHRLPGSDRRSRVRAA
                     CPDGDLVYDGLAFPGTLDAQTDPMAGRRLLRAARDGTRMTPLALDLVFGSVACTRGGV
                     WGLSDQGALPGNGGSRYLTEFQATGASRAVTPTLPSPLWSVVDLIPDDRLLVLSNTVP
                     LDAQEWRTQLYATAPDGSHPQLLDERPGLLDTTLFSPDGRYLLVAVNNASFTGPQVDQ
                     AIALVDMTGAAPPRELITARTTEGSEMLLYGMRGVWIRQGPHAGQVLLIWTDATGQVI
                     RQIDPEHPDAVVREVHTDRRYLGLHLLSADADGGLVLADLASAGPSYSRNSTLLYLDP
                     RGGLREMPVPFPEAGAFANSWTRAGRLVYATGLYGSQGEGPRFVVSSIPLDQFGVPGA
                     QPTEIYSGVLTQDGIWPVLPWRAGPGLLAYTTPQGQLHARTYTTGADIPLEAGVLGFS
                     MIESYAGDAAP"
     gene            1733..>2018
                     /locus_tag="VF276_07335"
     CDS             1733..>2018
                     /locus_tag="VF276_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606709.1"
                     /translation="MSDPAPLAPIEDLDAEPARAGWRARRGEAVLAALLVLAVIGFAG
                     GQWWDQQGRATHYQAGRRAAGAFDWAQATAEYGAAGTYRDAAQQAARAQAT"
BASE COUNT          269 a          738 c          739 g          272 t
ORIGIN      
        1 tcgtgctggg cgtgctgctc gtgctcggcg tcgtgagctt cgccgcgggg caggcgtggg
       61 accagcaggc gaaggtgacg cactaccagg cgggcagccg cgccgtgaac gcgctcgact
      121 gggcgcgcgc cgccgtcgag tacggcgcgg cgggggccta tcgcgatgca cccgcgcagg
      181 ccgcccaggc cgcgagcttc ctggcggaac gcgaccgcct gtacgcggcg gcgcaaaccg
      241 ccgcccgccg ctacgactgg cccgccgtgc tcgatctcct gccccgcctg cggcaactcg
      301 gccccgatta ccaggagacc cgccatctga ccgatgtggt ggcggcgctg gcgccgggcc
      361 tggccctgag cggcacggtc gccctgcgtg cgggcaacgg cgaccccggc ctctacagct
      421 acctgcccga cggctggcac cgcctgccgg gcagcgaccg ccgcagccgc gtgcgcgccg
      481 cctgccccga cggcgacctg gtctacgacg gtctggcgtt tcccggtaca ctcgatgcgc
      541 aaaccgatcc gatggctggg cggcgcctgc tgcgcgcggc ccgcgacggc acccgcatga
      601 cgccgctggc gctcgatctg gtgtttggct cggttgcctg cacccgtggc ggcgtgtggg
      661 gcctcagcga ccagggcgcc ttgcccggca acggcgggtc gcgctacctg accgagttcc
      721 aggcgaccgg tgctagccgc gccgtcacgc ccacgctgcc cagcccgctc tggtctgtgg
      781 tcgacctcat ccccgatgac cgcctgctgg tactgagcaa caccgtgccg ctggacgcgc
      841 aggaatggcg gacgcaactc tacgcgacgg ctcccgacgg cagccatccg caactgctcg
      901 acgagcgacc gggactgctg gacacgacgc tgttcagccc cgatggccgc tacctgctgg
      961 tcgccgtcaa caacgcatcc ttcaccgggc cgcaggtgga ccaggcgatc gcgctggtgg
     1021 atatgacggg cgccgcgccg cccagggagt tgatcaccgc gcgcaccacc gaaggctcgg
     1081 agatgctgct ttacgggatg cgcggtgtct ggatccgcca ggggccgcac gccggtcagg
     1141 tgctgcttat ctggaccgat gccaccggcc aggtgatccg ccagatcgac ccggagcacc
     1201 cggatgcggt ggtgcgggag gtccacacgg accggcgcta cctggggctg cacttgctca
     1261 gcgccgacgc ggacggcggc ctcgtgctgg ccgacctggc gagtgccggg ccgagttaca
     1321 gccgcaacag caccctgctc tacctcgacc cgcggggcgg cctgcgcgaa atgcccgtgc
     1381 cgttcccgga ggcgggcgct ttcgccaata gctggacgcg ggccgggcgc ctggtctatg
     1441 ccacggggct ttacgggagc cagggcgagg ggccacggtt cgtcgtgagc agcatcccgc
     1501 tggaccagtt cggggtgccg ggtgcccagc ccaccgagat ctacagcggc gtgctgacgc
     1561 aagacggcat ctggccggta ctgccctggc gcgccggtcc cggcctgctg gcctacacca
     1621 cgccgcaggg ccaactacac gcccgcactt acaccactgg cgccgacatc ccgctggagg
     1681 cgggcgtgct cggcttctcc atgatcgaat cctacgcggg tgatgcggcg ccatgagcga
     1741 tcccgcgccc ctggcgccca tcgaggacct ggacgcggaa ccggcgcggg ccgggtggcg
     1801 ggcgcggcgc ggggaggcgg tgctggccgc gctgctggtg ctggccgtga tcggcttcgc
     1861 gggcgggcag tggtgggacc aacagggccg ggcgacgcac taccaggcgg gccgccgggc
     1921 cgccggcgcg ttcgactggg cgcaggcgac ggcggagtac ggggcggcgg gcacctaccg
     1981 cgatgccgcg caacaggcag cgcgggcgca ggccacgc
//
LOCUS       DASUYC010000444         3035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1691121, whole genome shotgun sequence.
ACCESSION   DASUYC010000444 DASUYC010000000
VERSION     DASUYC010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1691121"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1520
                     /locus_tag="VF276_07340"
     CDS             <1..1520
                     /locus_tag="VF276_07340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX6606710.1"
                     /translation="LTAGPTTAELSSAQLKVISAQTNFDKVSSSASLAKQNAEKALDQ
                     SGNAVRDAQDQLNEASVINVPRQGQVNIVNPDGTFNSNATLAQIDKYRAALRAEQDAE
                     ANMHQAQLALDDARQQEIQSVAAAQATLDDAKKQLADLQSGPAAADLAAAQAAVDQAQ
                     NNLAKLQAPPAQADLVQAQAAVDQAKNNLAKLQAPPAQADLVQAQAAVDQAKNNLAKL
                     QAPPTHDDLVQAQAAVTQAQASLAKVKAGTAAADLAQAQSTVDQAKNNLNDLQAGATS
                     TDLETAQATVDQAKSNLSSAQLKLAQAVLTAPFDGVVADVPVTVGGQVGASAAVIDLL
                     DESSYHVDMNVGESDVSRVKVGQPVNLSFDALPGEVFTGTITYVAPKATVQQGVVSYL
                     ATATLDPKAAGSDIKPGMTTTAEAIVQQVSDALMVPNRAIRTEARQKVVYVLDGRNQI
                     RVPVQTGISNDQFTEITGDTPLREGDTLVLNTTTTTTQAPGGGGFINFGGAGRGR"
     gene            1537..2355
                     /locus_tag="VF276_07345"
     CDS             1537..2355
                     /locus_tag="VF276_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6606711.1"
                     /translation="MLEVQDLTKVYRMSAEVEVHALRGVSFTVDDGELVAVMGPSGSG
                     KSTIMNILGCLDRPTSGSYRIDDQEVASLSDAELAVVRNKRIGFVFQSFNLLPRTTAI
                     QNVELPMIYNRTGLAARERKARAKAALEVVGLGDRLDHKPQELSGGQQQRVAIARALV
                     NNPAIILADEPTGNLDSIASAEIMAIFQRLNKEQNITVVFVTHEPDIAAHTRRILRMR
                     DGHLVADEIVRNPVTADPEAVRQAALAEEAALHGGNGAGPLPHGPLLVVAGGEQ"
     gene            2352..>3035
                     /locus_tag="VF276_07350"
     CDS             2352..>3035
                     /locus_tag="VF276_07350"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6606712.1"
                     /translation="MSILESLSVAWASMRANKLRSILTMLGIIIGVAAVVALMGIGQG
                     AQQSITNNITQNGTNLLTITPGSFTQGGVRSGAGGAQTLTSEDADAIADPANCSYCTL
                     VAPEVRRQSSVVYGSQNNTYSISGTTPDYGTIRNMTLAEGDWFSTGDVNAATNVAVIG
                     ANVANDLFQGEDPLDKTVRINRLAFRVVGVAAPKGGTGFGSLDDGVYIPVTTAQRKLF
                     GGRQAAIAGH"
BASE COUNT          587 a         1069 c          993 g          386 t
ORIGIN      
        1 cgctgaccgc gggcccgacc accgccgaac tcagttcggc gcaactgaag gtgatctcgg
       61 cgcagaccaa cttcgacaag gtcagttcct cggcctcgct agccaagcag aacgcggaga
      121 aagcgctgga tcaatcgggc aacgccgtgc gcgatgccca ggatcaactc aacgaagcct
      181 ccgttatcaa cgtgccgcgc cagggccagg tgaacatcgt gaaccccgac ggcacgttca
      241 acagcaacgc cacgctggcc cagatcgaca agtaccgggc ggcgctgcgg gccgagcagg
      301 acgccgaagc taacatgcac caggcccagc tcgccctcga cgacgctcgc cagcaggaga
      361 tccagtcggt ggctgccgcg caggcgacgc tcgacgacgc caagaagcaa ctggccgatc
      421 tccagtccgg cccggcggcg gcggacctcg cggctgccca ggcggcggtg gaccaggcgc
      481 agaacaatct cgccaaactc caggccccgc cggcgcaggc cgatctggtg caggcgcagg
      541 ccgcggtgga ccaggccaag aacaatctcg ccaagttgca ggccccgccg gcgcaggccg
      601 acctggtgca ggcccaggcg gcggtagacc aggccaagaa caacctcgcc aagttgcagg
      661 ccccgcccac gcacgacgat cttgtgcagg cgcaggccgc ggtgacgcag gcccaggcca
      721 gcctcgcgaa ggtcaaggcc ggcaccgccg ccgccgatct ggcgcaggcg cagtccaccg
      781 tggaccaggc caagaacaac ctcaacgatt tgcaggccgg ggccacgagc actgacctgg
      841 aaaccgccca ggccaccgtg gaccaggcca agtcgaacct gtccagcgcc caactgaagc
      901 tcgcgcaggc cgtgctgacc gccccgtttg acggcgtggt ggccgatgtg ccggtcacgg
      961 tgggcgggca ggtcggcgcc agcgcggcgg tcatcgatct gctggatgag agcagctacc
     1021 acgtcgatat gaacgtgggc gaaagcgatg tcagccgcgt caaggtgggg cagccggtca
     1081 acctgagctt tgacgccctg cccggcgagg tcttcaccgg gaccatcacc tatgtggcgc
     1141 cgaaggccac ggtgcagcag ggcgtggtca gctacctggc gacggccacg ctcgatccca
     1201 aggcggcggg cagcgatatc aagcccggca tgaccaccac ggcggaggcc atcgtccagc
     1261 aggtgtcgga tgcgctgatg gtgcccaacc gcgccatccg caccgaggcc cgccagaagg
     1321 tggtctacgt gctcgacggc cgcaatcaga tccgcgtccc ggtgcaaacg ggcatcagca
     1381 acgaccagtt taccgagatc accggcgaca cccccttgcg cgaaggcgat accctggtgc
     1441 tcaacaccac cacgacgacg acccaggcgc ccggtggcgg cggcttcatc aacttcggcg
     1501 gcgcaggccg cggccgctag ccgggaggac aaggccatgt tggaagttca agacctgacc
     1561 aaagtctacc gcatgagcgc ggaggtggaa gtccacgccc tgcgcggcgt ctccttcacc
     1621 gtcgacgacg gcgaactggt ggccgtcatg gggccgtccg ggtccggtaa atcgaccatt
     1681 atgaatatcc tcggctgcct cgaccggccc acgagcgggt cctaccgcat cgacgaccag
     1741 gaagtcgcca gcctcagcga cgccgaactg gcggtggtgc gcaacaagcg catcggcttc
     1801 gtgttccaga gctttaatct gctgccgcgc accaccgcga tccagaacgt cgagctgccg
     1861 atgatctaca accgcaccgg gcttgctgct cgcgaacgca aagcgcgggc caaggcggcc
     1921 ctggaggtgg tcggcctggg cgaccggctc gaccacaagc cgcaggaact gtccggcggt
     1981 cagcagcagc gcgtggcgat tgcccgcgcc ctggtcaaca acccggcgat catcctggcc
     2041 gacgagccga cgggcaacct cgacagcatc gccagcgccg agatcatggc gatcttccag
     2101 cgcctgaaca aggaacaaaa catcacggtg gtcttcgtca cgcatgagcc ggacatcgcc
     2161 gcgcacacgc ggcgcatcct gcgcatgcgc gacggccacc tggtggccga cgaaatcgtc
     2221 cgcaaccccg tgacggccga tccggaggcc gtgcgccagg cggcgctggc ggaggaagcc
     2281 gcgctgcatg gcggcaacgg tgcgggcccc ttgccgcacg ggccgctgct ggtcgtggcc
     2341 ggaggcgaac aatgagcatc ctggagagcc tgagcgtggc ctgggccagc atgcgcgcca
     2401 acaagctgcg cagcatcctg accatgctcg gcatcatcat cggggtggcg gcggtggtcg
     2461 cgctcatggg catcggccag ggcgcgcaac agtcgatcac caacaacatc acccagaacg
     2521 gcaccaacct gctgaccatc acgccgggtt cgtttaccca gggcggcgtg cgtagcggcg
     2581 ccggcggcgc gcaaacgctc accagcgagg acgccgacgc catcgccgac ccggccaact
     2641 gctcgtattg caccctggtg gccccggagg tgcgccggca aagcagtgtc gtctacggta
     2701 gccagaacaa cacctactcg atcagcggca ccacgcccga ctacggtacc atccgcaaca
     2761 tgaccctggc cgaaggcgac tggttcagca ccggcgacgt gaacgcggcg accaacgtcg
     2821 ccgtgatcgg cgccaacgtc gccaacgacc tgttccaggg cgaggacccg ctcgataaga
     2881 ccgtccgcat caaccggctg gccttccgcg tggtcggcgt ggcggcgccc aagggcggca
     2941 cgggcttcgg ctcgctggac gacggcgtct atatccccgt gacgacggcc cagcgcaagc
     3001 tgttcggcgg gcgccaggcg gccatcgccg ggcac
//
LOCUS       DASUYC010000445         2868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_988675,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000445 DASUYC010000000
VERSION     DASUYC010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988675"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1480)
                     /locus_tag="VF276_07355"
     CDS             complement(<1..1480)
                     /locus_tag="VF276_07355"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX6606713.1"
                     /translation="MNRTIPAPAPPPDSPLRRQLRREYGWRWWVAPRPLAWIATLVYL
                     ALLVLLLTGDCRACADDDPRGALALGAILALLTLDRWEVRRWGERPPARVALALLAGR
                     ALLIEALAVAWGAWPGPAWPVLWPYALLPYTAFLYFGARGGVLAGLAVWGIIAARFTL
                     QALAHGTVITVYQGATTSQYLVPNVDWGEYISNATGYALLIFFVVTTSRIVAEERAYR
                     VRAESLLALLEAAQRQLRDYAAQTLAATQARNEAARAIHARLGQDLAATDADLATALA
                     LRSRDPAGAEQAVHAAKRRASEALRAVRRSVGTLRAGQDTVALPAAPALPAAPPPPSG
                     WRFWLAPRRFDELSTAVYVSIIPIWLASELHWAPGIMDTWDDVLFLGLVIGLYALDRI
                     EYRLFGEVPPPRVAAILLGVRMLAVAFGGVVLQVWWSFWFLPLIAYLAAVYFGSWRNY
                     LGNALIGVAGAVGIVALLGLSTIGTPDFDAAAIANSLIQISIV"
     gene            1764..2444
                     /locus_tag="VF276_07360"
     CDS             1764..2444
                     /locus_tag="VF276_07360"
                     /inference="COORDINATES: protein motif:HMM:NF012309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEX6606714.1"
                     /translation="MKAISRLLTGCMAVMLLVGAGLTTLPAQAQGTPTGATAALKDAQ
                     GTVVGTATLTEGAGGVHLQVQVQGFTGAASGEHGIHIHAVGVCEAPGFTTAGGHFNPA
                     AKKHGLQSAEGAHGGDLPNLVLDAAGSGSYQATNDRVTLAAGAANSIFDADGSAIVIH
                     AGPDDNVSDPAGNSGGRIACGVLQAAQIPTGMPRTGASSDATLWLALLGGAMLAGGWW
                     MTRRPARA"
     gene            2537..>2868
                     /locus_tag="VF276_07365"
     CDS             2537..>2868
                     /locus_tag="VF276_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606715.1"
                     /translation="MFQSTWLRWSALAGMLGGGAFVLAFLGSTLINDNTYPGPYSPVA
                     LLVSALILPALLLMIGGLTGLQARRLGGSGAPGGIAFAVTLAGLILLFLGFGGQLIEM
                     TGGSPVNGA"
BASE COUNT          496 a         1008 c          988 g          376 t
ORIGIN      
        1 tgacgatgct gatctggatc aagctgttgg cgatggcggc ggcatcaaag tccggcgtcc
       61 cgatagtgct caaacccagt agcgccacga tcccaaccgc ccccgcgact ccgatcagcg
      121 cgttccccag gtagttgcgc cagctcccaa aatagacggc ggcgaggtag gcgatcaacg
      181 ggagaaacca gaaactccac cagacttgca ggaccacgcc gccaaacgcg acggcaagca
      241 tccgcacgcc cagcaggatg gcggcgacgc ggggcggcgg cacctcgcca aacagccggt
      301 attcgatccg gtcgagcgcg tacaggccga tcaccaggcc gagaaacagc acatcgtccc
      361 aggtgtccat gatccccggc gcccagtgca actcgctcgc gagccagata gggataatcg
      421 acacatagac ggcggtggat agctcgtcaa agcggcgcgg cgccagccag aagcgccaac
      481 cgctgggcgg cggcggggcg gcgggcaggg cgggcgccgc cggcagcgcc accgtgtcct
      541 ggcccgcgcg cagggtgccg accgagcgcc ggacggcgcg cagcgcttcg ctcgcccggc
      601 gcttggccgc gtgtacggcc tgttcggccc cggcggggtc tcgcgaccgt agggccagcg
      661 ccgtcgccag gtcggcatcg gtggcggcca gatcctggcc cagacgcgca tggatcgccc
      721 gcgccgcctc gttgcgcgcc tgggtggccg ccagtgtctg ggcggcgtag tcgcgcagtt
      781 gccgctgggc ggcttccagc agcgccagca ggctctccgc ccgcacccgg tacgcccgtt
      841 cctcggccac aatgcgggat gtcgtgacca caaaaaagat gagcagggca tagcccgtgg
      901 cgttgctgat gtactcgccc cagtccacgt tgggcaccag gtactgcgat gtcgtggcgc
      961 cctggtagac cgtgatcacg gtgccgtgcg ccagggcctg gagggtgaac cgggccgcga
     1021 tgatccccca gacggccagc cccgccagaa cgccgccgcg cgcgccgaaa tacaggaaag
     1081 ccgtataggg cagcagcgcg tagggccaca gcacgggcca cgccggcccc ggccacgccc
     1141 cccaggcgac cgcgagcgcc tcgatcagca gcgcgcgccc ggccagcagc gcgagggcga
     1201 cccgcgccgg ggggcgctcg ccccaccgcc ggacttccca ccggtcgagg gtgagcagcg
     1261 ccaggatggc gcccagggcc agcgccccgc gcggatcatc gtcggcacag gcgcgacagt
     1321 cgccggtcag cagcaagacc agcagggcca ggtagaccag tgtggcgatc caggccagcg
     1381 ggcgcggcgc cacccaccag cgccatccat attcccggcg taattgccgg cgcagcggcg
     1441 agtccggcgg cggtgcgggc gcaggtatcg tgcggttcat aggaggagca taacaaaaag
     1501 gccggggttg ggcaataggc cggaagacac acccgcgccc gccggaagtc atatgacctt
     1561 tggcggcacc gccgccttcg tctgccgaac ggctgccgtg cgcccaccga gtcacaatcc
     1621 cgaatcttac ttccggcatg tacaaaatag gccgttccgc acatggccgc gcggcgggcc
     1681 ggcgctatgc ttaacgaggc tgcagcaccg gggacggtcc tccggcacag gcatcgaaac
     1741 caatccacac taaggagaca gacatgaaag cgatttctcg actactcacc ggctgcatgg
     1801 cggtcatgct gctggtcggg gccggcctca cgaccctccc ggcgcaggcg caaggcacgc
     1861 ccactggcgc caccgcggcg ctcaaggacg cgcaaggcac cgtagtgggc accgccaccc
     1921 tgaccgaagg ggcgggcggc gtgcacctcc aggtccaggt ccagggcttc accggagccg
     1981 cctcgggcga gcacggtatc catattcacg cggtaggggt gtgcgaggcg ccgggattca
     2041 ccacggcggg cgggcatttt aaccccgcgg cgaaaaagca cggcctgcag agcgccgaag
     2101 gcgcgcacgg cggcgacctc ccgaacctgg tcctcgacgc tgccggcagc ggcagctacc
     2161 aggccacgaa cgaccgcgtc acgctggcgg ccggggcggc caactcgatc ttcgacgccg
     2221 acggcagcgc catcgtcatc cacgccggtc cggatgacaa cgtgagcgat ccggcgggca
     2281 acagcggcgg acggattgcc tgtggcgtcc tccaggccgc gcagatcccg acgggcatgc
     2341 cacgcaccgg cgcgtcgagc gacgcgacac tgtggctggc cctgttgggc ggcgcgatgc
     2401 tcgccggggg ctggtggatg acgcggcgcc cggcccgcgc ctgattaacc aatagcgggg
     2461 gcgaaccaca caccgtcccc gccgctgcaa ccgcgactgt attcacaact gtgcgacacg
     2521 aagggagtaa acgaccatgt tccaatcgac ctggctccgc tggagcgcgc tggccgggat
     2581 gctggggggc ggcgcgtttg tgctggcgtt cctgggcagc accctcatca acgataatac
     2641 ctaccctggc ccctatagcc ccgttgcgct gctggtgagc gcgttgattc tgccggccct
     2701 actgctgatg atcggcggct tgaccggctt gcaggcccgg cgcctgggtg gatcgggtgc
     2761 gcccggcggc atcgccttcg cggtgacgct ggccgggctg atcctgctct tcctgggttt
     2821 cggcgggcaa ctaatcgaaa tgaccggcgg gtccccggtg aacggcgc
//
LOCUS       DASUYC010000446         3093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_43132,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000446 DASUYC010000000
VERSION     DASUYC010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43132"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="VF276_07370"
     CDS             complement(<1..806)
                     /locus_tag="VF276_07370"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6606716.1"
                     /translation="MGLSGRPRLSWNFRSRSEAEPVRVLVPPDVAGLRLPWTSRFSPT
                     SLTAHLTRHPGYGWVIPATGEYLIAEPWRRRDEIGGVLEIHARRGRGALLAQAAADLR
                     AAGVRMLLLPEGDWAAHARVYTEQGFVRLERVVYYQLLGLEGDPALTRPLPPLECALL
                     TGVQLGTVLAVDHAAFPWLWWNSEAEFRVYHAQAGVQIWLGWAGGVAVAYAGFTILDR
                     WAHLDRLAVDPTRHGQGYGAAMLDFALRRMAAVGVNRVTLSTQETNVQSQR"
     gene            complement(811..2472)
                     /locus_tag="VF276_07375"
     CDS             complement(811..2472)
                     /locus_tag="VF276_07375"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX6606717.1"
                     /translation="MPSHQPRVAKFTLLLLGLLLILPACGPLASAGPVARLAATGSPT
                     AANLKAADQVFTLIGSVDDPPTLDPALASDSTSAELIRHLFSGLVRLDDTLAVVPDLA
                     AALPELSADGRTYTFHLRPGVRWPDGRAVTADDVRYSLERATDPALAAPQPGTSLPAG
                     LYLNDIVGVPERLAGAPGPLKGVNVLAPDTIALTISTPRAYFLQKLVTFYVVDQRNVE
                     AGGREWYRHPQGTGPFRLQDWRSGDRITLVPNPTYYGGAPHLRQVNFLLGEAATGGLV
                     QYEQGLIDFVGVPADDVDRVQDPAGPLHGELHITPGLSTTYLGFNMNKPPFDDPKVRE
                     AFSLVVNRDKIARVMFNGRVEQAAGIVPTALPGYLPAVTVPAPNIARARQLLAESRYH
                     GASGLPRIAVYSNGGDIGPMLQQVFKDALGVEIEVRVQDWAEYLAGLHRGDYQAFISG
                     WEADWPEPSNFLEALFRSTSPENETGLRDPEVDSTLDRAAATPDPAPRYALYAAAEQR
                     ALAATPLVPLFHSVNYELLRSYVHGVTITPLGILNLKDAYIDRGH"
     gene            complement(2675..3025)
                     /locus_tag="VF276_07380"
     CDS             complement(2675..3025)
                     /locus_tag="VF276_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015083338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HEX6606718.1"
                     /translation="MLRLAADENVNSALIRGVLRRNPSLDLVRVQDVGLRGADDASVL
                     AWAAREGRVLITYDIKTVPPAAYARIAQDQPMPGVIAIPATVPLGTVIDDLVLLAVAT
                     EPGEWKGKIEYLPL"
BASE COUNT          505 a         1006 c         1096 g          486 t
ORIGIN      
        1 cgttgcgact ggacgttggt ctcctgggtg cttagcgtca cccggttgac gccgacggcg
       61 gccatccggc gcagggcaaa gtcgagcatg gcggccccgt agccctggcc gtgccgggtc
      121 gggtccacgg ccagccggtc caggtgcgcc cagcggtcca ggatagtgaa cccggcatag
      181 gccaccgcca ccccgccggc ccagcccagc cagatctgca ccccggcttg cgcgtgataa
      241 acccggaact cggcctcgga gttccaccag agccacggaa acgccgcatg atccaccgcc
      301 aggaccgtgc cgagttgcac gcctgtgagc agggcgcatt ccagcggcgg cagcgggcgc
      361 gtgagcgccg ggtcgccttc cagccccagg agctggtagt agaccacccg ctccagccgc
      421 acaaagccct gctcggtgta gacccgcgcg tgcgcggccc agtcgccctc cggtaacagc
      481 agcatgcgta cccccgctgc ccgcaggtcg gcggcggcct gggccagcag cgcgccgcgc
      541 ccgcgccggg catggatctc cagcaccccc ccgatctcgt cgcggcggcg ccacggctcg
      601 gcgatcaagt actcgccggt ggccgggata acccagccgt aaccggggtg gcgggttaga
      661 tgggcggtca ggctggtcgg gctgaagcgg ctggtccacg gcaggcgcag cccggccaca
      721 tcgggcggga ccaggacgcg caccggctcg gcctcgctgc gtgagcgaaa gttccaggag
      781 agccgcgggc gcccggagag tcccatccgc ctagtgcccc cggtcgatat aggcatcctt
      841 cagattcaag atgcccagcg gggtaattgt tacgccatgc acataggatc gcaacaactc
      901 gtaattcaca ctgtggaaca gcgggaccag cggcgtggcg gccagcgcgc gctgctcggc
      961 ggcggcgtag agggcgtagc gtggggctgg gtcgggcgtg gcggcagccc ggtccagggt
     1021 gctgtccacc tccgggtcgc gcaggccggt ctcgttctcg gggctggtcg agcggaagag
     1081 cgcctccagg aagttcgacg gctcgggcca gtcggcctcc catccgctga tgaaggcctg
     1141 gtaatcgccg cggtgcagtc cggccaggta ctcggcccag tcctgcacgc ggacctcgat
     1201 ctcgacgccg agcgcgtcct tgaacacctg ttgcagcatg gggccgatgt cgccgccgtt
     1261 cgagtagacg gcgatgcgcg gcaacccgct cgcaccgtgg tagcgcgact cggccagcaa
     1321 ctgccgtgcg cgggcgatgt tgggcgcggg caccgtgacg gcgggcaggt aacccggcag
     1381 cgcggtgggc acgatgcccg cggcctgctc gacccgcccg ttgaacatca cgcgcgcgat
     1441 cttgtcgcgg ttgactacca ggctgaacgc ctcgcgcact ttggggtcgt caaacggcgg
     1501 cttgttcatg ttgaagccca ggtaggtggt ggaaaggccg ggcgtgatgt gcagttcccc
     1561 atgcagcggg ccggcggggt cctgcacgcg gtccacgtcg tcggcgggca cgcccacgaa
     1621 gtcgatcagg ccctgctcgt attgcaccag accgccggtg gctgcctcgc ccagcaggaa
     1681 gttgacctgg cgcaagtggg gtgcgccgcc gtaataggtg gggttgggca cgagcgtgat
     1741 gcggtcgccg ctgcgccagt cttgcagccg gaaggggccg gtgccctgcg ggtggcgata
     1801 ccactcgcgg cctcccgcct cgacgttccg ctggtccacc acgtagaagg tgacgagctt
     1861 ttgcaggaag taggcgcgcg gcgtgctgat agtcagggcg atggtgtcgg gcgcgaggac
     1921 gttgacgccc ttgagcgggc cgggcgcccc ggccagccgt tcgggcacgc ccacgatgtc
     1981 gttcaggtag aggcccgcgg gcaggctggt gcccggttgc ggggcggcga gggccgggtc
     2041 ggtggcccgt tccagcgagt agcgcacatc gtccgccgtc acggcccggc catcgggcca
     2101 gcgcacgccg ggccgcaggt ggaaggtgta ggtccggccg tcggcggata gctcaggcag
     2161 cgccgcagcc agatcgggca ccacggccag cgtatcgtcc aggcgcacca ggccgctgaa
     2221 cagatggcgg atcaactcgg cagatgtcga atccgaggcc agcgcggggt ccagcgtggg
     2281 cgggtcgtcg acgctgccga tgagggtaaa gacctggtcg gcggctttga ggttcgcggc
     2341 ggtcgggctt ccggtcgccg ccaggcgggc caccggcccc gcgctcgcta acggtccaca
     2401 ggcgggcagg atcagcagca gcccgagcag gagcagtgtg aacttggcga cccgcggttg
     2461 gtgtgacggc atgggactcc cgctttcagc agatgccggg cgccccgcga gacgccctgg
     2521 ctgcctcatt gtaccagaga gccggaaacg gggtcaaggc aacagggcgg gatgctgatc
     2581 tggtttcggg catatcccag gtcgtctctg tgagaagccc tgattttgaa aactacatcc
     2641 acggcgataa atcagcagca gagcgtgaat ctgtttatag aggaaggtac tcgattttgc
     2701 ctttccattc acccggttcg gtggcaacgg ctaacaatac gagatcatca atcactgttc
     2761 ctaatggtac ggtagcagga atagcgatca caccgggcat tggttggtcc tgggcgatcc
     2821 gtgcatacgc ggctggcggc actgtcttaa tgtcataggt gattagcaca cgaccttcgc
     2881 gagccgccca ggcaagaacg ctggcgtcat ccgcaccgcg cagccccaca tcttgcactc
     2941 gcacaaggtc taaactggga ttgcggcgga gaacgccacg gattaatgcg ctgttgacgt
     3001 tctcatccgc agccaggcgc agcactgtct attggccctg ctgtgcacgt aaggccagaa
     3061 gccgctcgcg cagcggatcg gggtgctggc gag
//
LOCUS       DASUYC010000447         3730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_705573,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000447 DASUYC010000000
VERSION     DASUYC010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705573"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VF276_07385"
     CDS             <1..702
                     /locus_tag="VF276_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6606719.1"
                     /translation="NTSRAPLDRKEVRQALMYALDRKRIAETVTHAPAIVGSAGVVPP
                     ETPWFDPDLPQYGYDPARARTLLGNQPLTVNLIADATAREPELMRPMLAAAGITLKVQ
                     NVDAATRTQLLTEGNFAMALTSHIGVGGDPDYLRRWYAGEEANTFAQGSIFHNAQYTQ
                     LGEQEAATIDRAQRRAIVFQMQQILAEELPTIVLYHRRFYWVYDPSVFTPMATAGGLM
                     NGIPFPNNKLTLLGP"
     gene            complement(828..1007)
                     /locus_tag="VF276_07390"
                     /pseudo
     CDS             complement(828..1007)
                     /locus_tag="VF276_07390"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
     gene            1428..>3730
                     /locus_tag="VF276_07395"
     CDS             1428..>3730
                     /locus_tag="VF276_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HEX6606720.1"
                     /translation="MSQETTRPAATANSEETVSVANVAFEHRRDTLGIGTAEPRLSWI
                     VTTQRPDWFQAAYEIEAVAPDGDRQDGTGLVQSDQSVLVAWPFAPLQSRERRAVRVRV
                     HGTDGSMSDWSAPYPVEVGLLEPGAWSARFVAPGWDEDTSHAQPCPLLRREFAIRPGV
                     RQARLYITALGVYEAQINGAVVGDHVLDPGWTSYEYRLRYQTFDVTGMLRAGHNAIGA
                     ILADGWYRGRLGFGGGRRNIYGDRLALLAQLEIAYADGTVERVVTDESWRAATGPILA
                     ADLYDGETYDARLERPGWSAPGYDAADWAAVVPLDRDLGTLVAPSGPPVRRMETVAPV
                     AILQSPAGRTLVDFGQNLVGRVRFTVQGPAGTTITLRHAEVLEAGELGTRPLRHAAAT
                     DSYTLRGGEAETWEPRFTFHGFRYAEVQGWPGELQAGDLRAVVCHSDMERTGWFECSD
                     PLVNRLHENVVWSMRGNFLDIPTDCPQRDERLGWTGDIAAFTPTACFLYDCAGFLQSW
                     LADLAVEQQAANGIVPVVVPNILDRVPFPAAAWGDAATIVPWVLYQRFGDAGILAAQF
                     HSMRAWVDAVAAIAGPNHLWDEGFQFGDWLDPSAPPDRPAQARTPPALVATAYLARSA
                     ELTGQAATVLGRAADAEHYLGLAAAVRAAFAAEYITAAGRLLSDSTTAYALALGFALL
                     PDEAQRRHAGKRLAELARASGYHISTGFVGTPLICDALCGVGEYDAAYRLLTQQDCPS
                     WLYPVTMGGTTIWERWDSMLPDGAINPG"
BASE COUNT          567 a         1343 c         1260 g          560 t
ORIGIN      
        1 aacacgtcgc gcgcgccgct ggaccgcaag gaggtgcgcc aggcccttat gtacgcgctc
       61 gaccgcaagc gcatcgccga gaccgtgact cacgccccgg ccattgtcgg cagcgcgggc
      121 gtggtgccgc cggagacgcc ctggttcgac cccgacctgc cgcagtacgg ctacgacccc
      181 gcgcgcgccc gcaccttgct gggcaaccag ccgttgaccg tgaatctgat cgccgacgcg
      241 acggcccgtg agcccgaact gatgcggccc atgctggcgg cggcgggcat cacgctcaag
      301 gtgcagaacg tggacgccgc cacccgcacc cagttgctga ccgagggcaa cttcgcgatg
      361 gcgctgacca gccacatcgg cgtcggcggc gaccccgact acctgcggcg ctggtacgcg
      421 ggcgaggaag cgaacacctt tgcccagggg tcgatcttcc acaacgcgca gtacacgcag
      481 ttgggtgagc aggaggccgc gaccatcgac cgggcgcagc ggcgcgccat cgtcttccag
      541 atgcagcaga tcctggccga ggagttgccg acgattgtgc tctaccatcg ccggttctac
      601 tgggtgtacg acccgagcgt gttcacgccc atggcgacgg cgggcgggct gatgaatggg
      661 atccccttcc ccaacaacaa gctcaccctg ctcggcccct gacggcgggc cagggcgcga
      721 tgccgggtga tcgcgctgca ggccggcccc gtccgccggg atacggcgct acgggacaga
      781 gcaataccgg catggcctgt cggccttgcc ggccatccgg gccggcgtta cgcggcgcgc
      841 cccggcacga ccgtttccgc ctgcggcacg tacccggtcc ggcccagggc gatccgcagg
      901 tccgccaggc gcacccgatc aggatcgaaa gtgaccgtcg ccgattgccg ggcaagatcg
      961 acagcgacga aatgcgtgcc cggcacggcc tctagcgcgg cttcgaccga agcccggcaa
     1021 acctctcaag gcatgcccag gccctggaag ataaccgtgc tttcgttggc cgcgggtggt
     1081 ggcgcggatg cggcgtcatg ctcggtcttc ttgaaaaaat ccaggatgcc catggctgtc
     1141 ctccttgtgt gttcgtgcag cagttgcccg gcgcccggcg tgccgccggc gctgccccgt
     1201 gcaccatagc ctccttgagt acagaagcaa catacgtgcc ggttgccccg cgccccacca
     1261 tctgccggga ccggaacagc gcgatgggcg caccgcagat atgtggcagc agatatccga
     1321 ttacggcccg ccctggatgg ccgttctgcg ggcgttgtgg tatgctgggg tgcagaagcc
     1381 ccgccgcgcc ggccggttcg attccgatag ccagaaggat catagagttg agccaggaaa
     1441 cgacgagacc cgcagctaca gcaaacagtg aagaaaccgt ctccgtagca aacgtcgcat
     1501 ttgagcatcg ccgggacacg ctgggcatcg gcacggccga gccccggctc tcctggatcg
     1561 tcaccaccca acgcccggac tggttccagg ccgcctacga gatcgaggcc gtggcgccgg
     1621 acggcgaccg gcaggatgga accgggctgg tccaatcgga ccagtcggtg ctggtggcct
     1681 ggcccttcgc gccgctgcag tcgcgggagc ggcgcgccgt gcgcgtccgg gtccacggca
     1741 ccgatggttc catgtcggac tggagcgcgc cctacccggt cgaggtcggg ttgctggaac
     1801 cgggcgcctg gtcggcgcgc tttgtcgccc ccggctggga cgaagatacc agccacgcgc
     1861 agccgtgtcc gttgctgcgt cgggaattcg cgatccgccc aggcgtgcgc caggcgcggc
     1921 tgtacatcac ggccctcggc gtgtacgaag cacagatcaa cggcgccgtg gtcggcgatc
     1981 atgtgctcga ccccggctgg accagctatg aataccgcct gcgctaccaa acgttcgacg
     2041 tgaccggcat gctgcgcgcg ggccacaacg ccatcggcgc catcctggcc gacggctggt
     2101 atcgcggccg gctgggcttc ggcggcggtc gccgcaacat ctacggcgat cggctcgcgc
     2161 tactggccca actggagatc gcctatgccg acggcacggt cgagcgcgtt gtcaccgacg
     2221 aaagctggcg ggcggccacc ggacccatcc tcgccgccga tctctacgac ggcgagacct
     2281 acgacgcgcg cctggagcgc cccggctggt ccgcgcccgg ctacgatgcg gcggattggg
     2341 ccgctgtcgt gccgctcgac cgggatctcg gcaccctcgt cgcgccgtcc ggcccgccgg
     2401 tgcgccggat ggagacagtc gcccccgtcg ccatcctcca atccccggcc ggtcgcacac
     2461 ttgtcgattt cggccagaac ctggtgggcc gcgtgcggtt cacggtgcaa ggccccgcgg
     2521 gcaccacgat caccctgcgc cacgccgaag tgctggaggc gggtgagctg ggcacgcgac
     2581 cgttgcgcca cgccgccgcc accgactcct acacgctgcg cggcggcgag gcggagacct
     2641 gggagcctcg cttcacgttt cacggcttcc gttatgcgga ggtgcaaggc tggccgggcg
     2701 agttgcaggc cggcgatctg cgcgccgtcg tctgccactc ggacatggaa cgcaccggct
     2761 ggttcgagtg ttcggacccg ctggtcaacc ggctgcacga gaacgtcgtc tggagcatgc
     2821 gcggcaactt cctcgacatc cccaccgatt gcccgcagcg cgatgagcgc ctgggctgga
     2881 cgggtgacat cgcggccttc acgcccaccg cctgcttcct ctatgattgc gccggcttcc
     2941 tgcaatcctg gctggccgat ctcgcggtcg agcaacaggc ggccaacggg atcgtgccgg
     3001 tcgtcgtgcc caatatcctg gaccgggtcc cattccccgc ggcggcctgg ggcgacgccg
     3061 cgaccatcgt gccctgggtg ctgtaccagc gtttcgggga cgccggaatc ctggccgccc
     3121 agttccacag tatgcgcgcc tgggtcgacg ccgtggccgc tatcgcgggg ccaaatcacc
     3181 tgtgggacga gggcttccag ttcggtgact ggctcgaccc gagcgcgccg ccggaccgcc
     3241 ccgcgcaagc acgcacgccc ccggccctgg tcgccaccgc gtacctcgcc cgctcggcag
     3301 agctgaccgg ccaggccgcg accgtcctcg ggcgggccgc ggatgcggag cactatctgg
     3361 ggctggccgc cgccgttcgg gctgccttcg ccgccgagta catcacggcg gcgggccggc
     3421 tgctcagcga ctccacgacg gcctatgcgc tcgccctcgg gttcgccctg ctcccggacg
     3481 aagcccagcg gcggcacgcc ggcaagcgcc tggcggaact ggcgcgggcc agtggctacc
     3541 acatcagcac ggggttcgtc ggcacgccgc tgatctgcga cgccctgtgc ggcgtgggcg
     3601 agtacgacgc cgcatatcgc ctgctcacgc agcaggattg cccgtcctgg ctctaccccg
     3661 tcaccatggg cgggacaacc atctgggagc gctgggacag catgctgccg gacggcgcga
     3721 tcaaccccgg
//
LOCUS       DASUYC010000448         4567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1922223, whole genome shotgun sequence.
ACCESSION   DASUYC010000448 DASUYC010000000
VERSION     DASUYC010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4567
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1922223"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="VF276_07400"
     CDS             <1..699
                     /locus_tag="VF276_07400"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEX6606721.1"
                     /translation="FAAAGPITYTGARRQGNGADLHANRANRAPTIDGKLDDWQGATR
                     WPAQYRVTDLSGAKNAADLSANFMIGYDTSGFYIGATITDDVHVQNKLTRGYELWKGD
                     DVELWFDTDLPGDFKVDPANQDDVQIGLSPGDFGQLEPEAAVFVPKGAAAGDLGIRVA
                     AAPRPDGHGYTLEAAIPWAALQHMKPQPGGAIGFCASAGDNDVPGTAQQQHMVSTCSR
                     MKWNVPTTFGMLYW"
     gene            complement(703..1140)
                     /locus_tag="VF276_07405"
     CDS             complement(703..1140)
                     /locus_tag="VF276_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX6606722.1"
                     /translation="MNQRQALPINSPQAIEQALAGKTIAVVGLSSNPQSPSYKIAAYL
                     QGQGYHIIPVNPTEQEVLGERAYPSLRDVPVPVDVVDIFRRADAVPGIVEDAIAVGAP
                     TVWMQLGIVNEAAAQRAHDAGLNVVMDHCMRVEHRLRHGAQKM"
     gene            complement(1261..2442)
                     /locus_tag="VF276_07410"
     CDS             complement(1261..2442)
                     /locus_tag="VF276_07410"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEX6606723.1"
                     /translation="MQDTLRTRLIGELDNLREKHLYRKLRVVSGEQKAVCVIDGKEVI
                     NLSSNNYLGLDTHPYLRQKAEEAIQKLGVGSGAVRTIIGTMDIHQELEERLAKFKHTE
                     ATLTFQSGFTANTGTIGALMEDGDAIISDELNHASIIDGIRLSKAQRYIYRHSDMNHL
                     EEQLKAAQGKRTRLIITDGVFSMDGDICKLPEIVELAERYGAATFVDDAHSSGVLGQN
                     GRGSVNHFGLDGRVDIQVGTLSKAMGALGGYVAGVQELRDFLIHRARPVLFSTSHPPS
                     VVATCIAAIDIMEREPELIERLWDNARYFKAGLNRLGFDTGHSETPITPVIAGSAEKA
                     MRLSDRLFELGVFAQGIGFPTVPDAKSRVRTIVTATHTRANLDAALAAFEQVGRELAL
                     I"
     gene            complement(2473..3516)
                     /gene="tdh"
                     /locus_tag="VF276_07415"
     CDS             complement(2473..3516)
                     /gene="tdh"
                     /locus_tag="VF276_07415"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013172749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEX6606724.1"
                     /translation="MPELMRAVLKAEAAPGAVLAQVPVPTVGPDDILVRVRAASMCGT
                     DLHIYNWDPWAQSRFHKPPMVFGHECCGDVVEAGRNVANVQVGDFVSLESHITCGHCL
                     QCRTGQGHICANVQILGVDRPGVYAEYVSVPAKVAWKNPPDMPVEVASIQEPFGNAVH
                     TVFSCDIPTKRVAVIGCGPIGLWAIAIAKAAGASAVYAVDINPVRLALAEQEGATLVI
                     NSRETDPVATVRAATDGEGVDVMLEMSGNPRAISQGFEMLRYGGEAALLGLTARPVEI
                     DLNTAVIFKGARVHGISGRRIWDTWYRTRGLILSGAVDPTCVITHRRDLAEFDKAFAM
                     MNSGEAAKVVLFP"
     gene            3762..4481
                     /locus_tag="VF276_07420"
     CDS             3762..4481
                     /locus_tag="VF276_07420"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HEX6606725.1"
                     /translation="MPKTAGKKPPVNTPPVQTRNQQAAIRRQMDETRQATRAQAQARR
                     EAQAAAARAARLRRRVLMSVGTGLVVVLVVGGLFFWYNDTHPGQSFPDQGHTHVASAT
                     APHAPYTTDPPTSGPHLDGLAPWGVSDKTLDPVEYVHNMEDGGVIVQYRPDLPAADVA
                     RLKTIVQSYDNIYVSMALPNGTSTQINQHVLMVPYPTLKEPVVLTAWTRMQRFPTLDE
                     NGIRHFIDMYRGIDHHIAGQG"
BASE COUNT          748 a         1488 c         1552 g          779 t
ORIGIN      
        1 ttcgccgccg ccgggccgat cacctacacc ggcgcccgtc gccagggcaa tggcgccgac
       61 ctgcacgcca accgcgccaa ccgcgcgccc accatcgacg ggaagctgga cgactggcaa
      121 ggcgcaaccc gctggcccgc ccagtatcgc gtcaccgatc tctccggcgc aaaaaacgcc
      181 gccgacctca gcgccaactt tatgatcggc tacgacacca gcgggttcta tatcggcgcc
      241 acgatcaccg acgacgtgca cgtgcagaac aagctgactc gcgggtacga gctctggaaa
      301 ggcgacgacg tcgaactctg gttcgacacc gacctgcccg gcgactttaa ggtcgatcct
      361 gccaaccagg acgatgtcca gatcggtctc tcgcccggcg attttgggca gttggagccg
      421 gaggcggcgg tgttcgtgcc caagggggcg gcagccggcg acctcggcat ccgggtggcc
      481 gccgcaccgc ggcccgacgg ccacggttac acgctggagg cggccatccc ctgggcggcc
      541 ctgcagcaca tgaagcccca accgggcggc gccatcggct tctgcgccag tgcgggcgac
      601 aacgatgtgc ccggcacggc gcagcagcag cacatggtca gtacgtgcag ccgcatgaag
      661 tggaacgtgc cgacgacgtt cgggatgctc tactggtagc cgctacatct tttgcgcccc
      721 gtggcgcagc cggtgctcca cgcgcatgca gtggtccatg acgacgttta ggcccgcgtc
      781 gtgggcgcgc tgggcggcgg cctcgttgac gatgccgagt tgcatccaga cagtgggcgc
      841 gcccacggcg atcgcgtcct ctacgatgcc gggcacggcg tctgcgcggc ggaagatgtc
      901 taccacgtcc accggtactg gcacgtcgcg taaggacgga taggcccgct cgcccagcac
      961 ttcctgttcc gtggggttca ccggtatgat gtgatagccc tggccttgca ggtacgcggc
     1021 gatcttgtaa ctgggacttt gcgggttgct ggagaggccg accacggcga tggtcttgcc
     1081 ggccagggct tgctcgatgg cctgcggact gttgatcggt aatgcctgtc gctgattcat
     1141 cattgcggct cctttccctc ctgccagagc aagccaggtg ccgcgggcgc cgcacccagc
     1201 acagcgcggc ccgctcccca gcgcgggaag tgggccgttt gcgtggcgcg ggccgggacg
     1261 ctagatcagc gcgagttcgc gacccacctg ctcgaaggcg gccagggccg cgtcgaggtt
     1321 ggcgcgggta tgggtggcgg tgacgatggt acgcacgcgc gacttggcgt cgggcacggt
     1381 ggggaagccg atgccctggg cgaacacgcc tagctcgaac agccggtcgc tcaggcgcat
     1441 ggccttctcg gcgctgcccg cgatgaccgg ggtgatcggc gtttcgctgt ggccggtgtc
     1501 gaagcccagc cggttgaggc ccgccttgaa gtagcgcgcg ttgtcccaga gccgctcgat
     1561 cagttccggc tcgcgctcca tgatgtcgat ggcggcgatg caggtggcga cgacactcgg
     1621 cgggtgcgag gtgctgaaga ggaccgggcg cgcgcggtgg atcaggaagt cgcgtagttc
     1681 ctgcaccccg gcgacatagc cgcctagcgc gcccatggcc ttggagagcg tgcccacctg
     1741 gatgtccacc cgcccgtcga ggccgaagtg gttgacgctg ccgcgcccgt tctggcccag
     1801 cacgccactg gagtgggcgt cgtccacgaa ggtcgcggcg ccgtagcgct cggccagttc
     1861 cacgatctcg ggcagcttgc agatgtcgcc gtccatgctg aacacgccgt cggtgatgat
     1921 caggcgcgtg cgtttgccct gggccgcctt gagttgctcc tccaggtggt tcatgtcgct
     1981 gtggcggtag atgtagcgct gcgccttcga caggcggatg ccgtcgatga tcgaggcgtg
     2041 gttcagttcg tcgctgatga tggcgtcgcc gtcttccatc agcgcgccga tggtgccggt
     2101 gttggcggtg aagcccgact ggaaggtgag cgtggcctcg gtgtgcttga acttggcgag
     2161 gcgctcttcc agctcctggt ggatgtccat cgtgccgatg atggtacgca ccgcgccgct
     2221 gccgacgccc agtttctgga tcgcttcctc ggctttctgg cgcaggtagg ggtgcgtatc
     2281 caggcccagg tagttgttcg aggagaggtt gatcacctct ttgccgtcga tcacgcagac
     2341 ggccttttgc tcgccggaga cgacgcgcaa cttacgatag agatgcttct cgcgcaggtt
     2401 gtccagttcg ccgatcaggc gggtacgtag tgtgtcttgc atggctgatg ctccttatgg
     2461 cgccgggccg gctcagggaa acagcacgac cttggcagcc tctcccgagt tcatcatagc
     2521 gaaggctttg tcgaattcgg ccaggtcgcg ccggtgggtg atgacgcagg tgggatcgac
     2581 cgcgcccgac aggatcaggc cgcgggtgcg gtaccaggtg tcccagatgc gccggcccga
     2641 aatgccgtgg acccgcgccc ccttgaaaat gacggcggtg ttcaggtcga tctccaccgg
     2701 gcgggcggtc aggcccagca gcgcggcctc gccgccgtag cgcagcatct cgaagccctg
     2761 cgagatggcg cgggggttgc ccgacatctc cagcatcacg tccacacctt cgccgtcggt
     2821 ggccgcgcgc acggtggcga ccgggtcggt ctcgcgcgag ttgatcacca gtgtggcgcc
     2881 ttcctgctcg gccagggcca ggcgcaccgg gttgatgtcc accgcgtaga ccgccgaggc
     2941 gcccgccgcc ttggcgatgg cgatggccca cagcccgatc gggccgcagc cgatgacggc
     3001 cacgcgcttg gtggggatgt cgcacgagaa gaccgtgtgg acggcgttgc cgaacggctc
     3061 ctggatggat gcgacctcga ccggcatgtc gggcgggttc ttccaggcca ctttggccgg
     3121 gacgctgacg tactcggcgt agacgccggg ccggtccacg cccaggatct gcacgttggc
     3181 gcagatatgg ccctggccgg tgcggcattg caggcagtgg ccgcaggtga tgtggctttc
     3241 cagggacaca aaatcgccga cctgcacgtt ggcgacgttg cggccggctt ccacgacatc
     3301 gccgcagcat tcgtggccga agaccatggg cggtttatgg aagcgcgact gcgcccaggg
     3361 gtcccaatta tagatatgaa gatcggtgcc gcacatgctg gcggcgcgca cgcggaccag
     3421 gatatcgtcc gggccgacgg tgggcaccgg cacctgggcc aggacggcgc cgggggcggc
     3481 ttcggccttc agcacggcgc gcatcagttc gggcatacga ctctgacctc ctcgtcagat
     3541 ctgccgccgg gggccgtcgt tggccgggcg ggacaagcgg gttcgggccg ggaggcacgt
     3601 cccgcgcggg attcgcatca ttgagcgggc cgggttgggg ctgagacagt atagcacccg
     3661 cccaagaagg gtgtcaagac ttgacatccg cccgaggtct cgcctatact ttgggacgat
     3721 gccccttgcg tcaacttcca gggaacagag aaagggaaag tatgcccaag acggccggca
     3781 agaaaccccc cgttaatacg cccccggtgc agacccgtaa ccaacaagca gccatccgcc
     3841 ggcaaatgga cgagacgcgc caggccaccc gcgcgcaggc gcaagcccgc cgggaagccc
     3901 aggccgctgc ggcccgcgcc gcgcgcctcc gccgtcgcgt cctcatgagc gtgggcaccg
     3961 gcctggtcgt cgtgctggtg gtgggcgggc tcttcttctg gtataacgac acgcatcccg
     4021 gtcagagttt cccggaccag ggccacaccc acgtggcgag cgctaccgcg ccccacgcgc
     4081 cctatacgac cgacccgccg acatccggcc cgcatctcga tggtctggct ccctggggcg
     4141 tctccgataa aactcttgat ccggtggaat acgttcacaa catggaggac ggtggcgtta
     4201 ttgtccaata tcgccccgat ctgccggccg ccgatgtcgc gcggctcaag actatcgtgc
     4261 agagctacga caacatatac gtgagcatgg cactgcccaa cggcaccagc acccagatca
     4321 atcagcatgt gctgatggtg ccgtatccca cgctgaaaga gccggtcgtg ctgacggcgt
     4381 ggacgcgtat gcagcggttc ccgacccttg acgagaacgg catccgccat ttcatcgata
     4441 tgtaccgtgg catcgatcat cacatcgccg ggcaggggta ggccccccgc gaccttggtt
     4501 gaggagaaac aggacaatga gtaaagaacc gaccggcaag cccgtgggcg tcgccaatcg
     4561 aacgacg
//
LOCUS       DASUYC010000449         3223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1210021, whole genome shotgun sequence.
ACCESSION   DASUYC010000449 DASUYC010000000
VERSION     DASUYC010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210021"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2536)
                     /locus_tag="VF276_07425"
     CDS             complement(<1..2536)
                     /locus_tag="VF276_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018237877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX6606726.1"
                     /translation="MMRWIVGASLKFRFLVLGIAAAVMFFGVARLREMPVDVFPEFAP
                     PRVEIQTEALGLTTTEVEALISVPLEEALSGTPRLDIMRSKSVPGLSSILLIFEPGTD
                     LILARQLVQERIALAASTLPNVSRPPTLLQPLSSTSRVMKIGLSSKDHSLIDMSELTR
                     FKIRPRLMSVPGVANVAVWGDRKRQLQVQVDPERLGAHDLSLNEILKITGDSLGVGTL
                     KYSNGTTIGTGGFIDTPNQRLGVQHVLPILSPDDLGRVTIKVRDGQAIRLGDVTTVVE
                     DHPPLIGDAVINDGEGLLLIVEKFPWGNTLEVTRGVEEALAVLQPGLPGIAIDSTIFR
                     PATFIETALDNLTAALIIGCILVALVLGAFLFEWRTALISLVAIPLSLVAGGLVLYLR
                     GASINTMILAGFVIAVGVVVDDAIIDVENIIRRLRQHRREGSSKSTASIILEASLEVR
                     SAIIFATLIDVMALLPIFFMQGLSGAFFQPLALSYALAVLASMLVALTVTPALALLLL
                     ARAPIERRESPLVRVLQGGYTWILARLIRSPRPAYATVVVIVLLGLAVVPSLGQSLLP
                     SFKERDFLMHWVTTPGTSHPEMYRITTEASKELRAIPGVRNFGSHIGRAIQADEVVGI
                     NFTENWISVDPAADYDQTLAAVQETVDGYPGLYRDVQTYLKERIREVLTGASNAVVVR
                     IYGPDLAVLRQKAEEVRQALKGVDGVVDLHVELQVEEPQVEIKVDLPAAQHYGLKPGD
                     VIRAAATVVNGVEVGDIFWGGKVYDVNVLGTLATRHSLTNMRELLLEAPNGGRVRLGE
                     VASVNVVPVPNIINREGVSRRIDVGLSVRGRDLGAVARDVEARLLTV"
     gene            complement(2563..>3223)
                     /locus_tag="VF276_07430"
     CDS             complement(2563..>3223)
                     /locus_tag="VF276_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606727.1"
                     /translation="PPAGITPIAGSDLHNVVLSEQAMQRLKIQTAPVREEPVIRKRSV
                     GGAVSLPDAGPPDTTTKPGAGGVWVRVPLPVADLGKVDRSQPVRVRPLDSADAGVPGR
                     LVETRAPEPKAVTGDLYYVVDSAGHGLSAGQRVHVDLTLAGSATPRKLIPYAAVVYAT
                     HGDTWAYTSNAPRTFVRHRVVVDYIEGDMAVLSEGPPTGTAVVTVGGAELLGTEFGVG
                     H"
BASE COUNT          551 a         1122 c         1044 g          506 t
ORIGIN      
        1 ccaccgtcag caagcgcgct tccacgtcgc gcgccacggc gcccagatcg cgcccgcgca
       61 cgctcagccc cacgtcgatg cgccgcgaga cgccttcccg gttgatgatg ttgggcaccg
      121 gcaccacgtt cacggaggcg acttccccta agcggacacg tccgccattg ggtgcttcca
      181 gcagcagttc gcgcatgttg gtcaggctgt ggcgggtggc gagggtgccg agcacgttca
      241 cgtcgtacac cttgccgccc cagaagatgt cgcccacttc gacgccgttc accacggtgg
      301 ccgccgcgcg aatgacatcg ccgggtttga gcccatagtg ctgggccgcc gggagatcga
      361 ccttgatctc gacctggggc tcctctacct gcaactcgac gtgcagatcg acgacgccgt
      421 cgacgccctt aagcgcctgg cgcacctcct cggctttctg gcgcagcacg gccaggtcgg
      481 ggccgtagat gcgcaccacc acggcgttgc tggcgcccgt cagcacctcg cggatgcgct
      541 ctttgagata ggtctgcacg tcgcgataga ggccggggta gccgtccacg gtctcctgca
      601 cggcggccag cgtctggtcg tagtcggcgg ccggatcgac gctgatccag ttctcggtga
      661 aattgatgcc gacgacttca tccgcctgga tggcccgccc gatgtgcgag ccgaagttgc
      721 gcacgcccgg aatggcgcgc aactccttgc tcgcctcggt cgtgatgcgg tacatttccg
      781 ggtgcgacgt gccgggcgtc gtcacccagt gcatgaggaa gtcccgctct ttgaacgacg
      841 gtaacagcga ctgccccagc gagggcacca cggccagacc cagcagcacg atgaccacga
      901 cggtggcgta cgccgggcgt gggctgcgga tcagccgggc caggatccag gtgtagccgc
      961 cctgcagcac gcgcaccagc ggcgactcgc ggcgctcgat cggcgcgcgg gccagcagca
     1021 acagggccag ggccggggtg acggttagcg ccaccagcat cgaggccagc accgccagcg
     1081 cgtaggacag cgccagcggc tggaaaaacg cccccgacag cccctgcata aagaagatag
     1141 gcagcagggc catcacgtcg atcagcgtcg cgaagatgat ggcgctgcgg acctccagcg
     1201 acgcctccag gatgatactc gccgtcgatt tgctgctgcc ctctcgccgg tgctgccgca
     1261 agcgccggat gatattctcc acgtcgatga tggcgtcgtc gacgactacc cccacggcga
     1321 tcacgaaccc cgccaggatc atcgtattga tgctggcccc gcgcagatag agcaccaacc
     1381 cgccggccac cagcgacaac gggatcgcca ccaggctgat cagcgccgtg cgccactcga
     1441 acaggaaggc gcccagcacc agcgccacga gaatacaccc gatgatcagc gccgcggtca
     1501 ggttatccag cgccgtttcg ataaacgtcg ccgggcggaa gatggtggag tcgatcgcga
     1561 taccgggcag gcccggctgc aacacggcca gcgcttcctc gaccccgcgg gttacttcca
     1621 gcgtattgcc ccagggaaac ttctccacga tgagcagcag gccctcgccg tcgttgatca
     1681 cggcgtcgcc gatgagcggg ggatggtcct ccacgaccgt ggtcacgtcg cccaggcgga
     1741 tcgcctgccc gtcgcgcacc ttgatcgtca cgcggcccag gtcgtcgggc gacaagatgg
     1801 gcaagacgtg ctggaccccc agccgttgat tcggcgtgtc gataaacccg ccggtgccga
     1861 tggtcgtgcc gttggaatac ttcagggtgc cgacgcccag ggaatccccg gtgatcttga
     1921 ggatctcgtt gagcgaaaga tcgtgcgcgc ctagccgctc gggatcgacc tgcacctgca
     1981 gttgccgctt gcggtcgccc caaacggcca cgttcgccac gccgggcacg ctcatcaggc
     2041 gggggcggat cttgaaccgg gtcagttccg acatgtcgat cagcgagtgg tccttggagg
     2101 acaggccgat cttcatgacc cggctggtcg aggacagggg ttggagcagc gtcggcggac
     2161 gcgacacatt gggcaacgtc gatgccgcca gcgcgatgcg ctcttgcact aattgccggg
     2221 ccaggatgag atcggtgccg ggctcgaaaa tgaggaggat cgacgacaga ccgggcaccg
     2281 atttcgagcg cataatatcg agccggggcg tgccgctgag cgcctcctcc agcggcacgc
     2341 tgatgagggc ttccacctcg gtggtggtga gccccagcgc ctcggtctga atctccacac
     2401 gcgggggcgc gaactcggga aacacgtcca ccggcatctc gcgcagccgg gcgacgccga
     2461 agaacatcac cgccgccgcg ataccgagca ccaggaagcg gaatttcagg ctcgccccaa
     2521 cgatccaacg catcatggct ggagatctcc tctcctagct aatcagtggc cgacgccgaa
     2581 ctcggtgccg agcaactcgg ccccaccgac ggtcacaacc gccgtcccgg tgggcggccc
     2641 ctccgacagg acagccatgt ccccctcaat gtagtcgacc acgacgcggt gccgcacgaa
     2701 ggtgcggggt gcgttgctgg tgtaggccca ggtatcgccg tgcgtggcat agaccacggc
     2761 ggcatagggg atcagtttgc gcggcgtggc gctccccgcc agcgtcaggt ccacatgcac
     2821 gcgctgcccg gcgctcagcc catggccggc gctgtccacc acatagtaca gatctcccgt
     2881 cacggctttc ggctcgggcg ccctggtctc gaccaggcgg cccggcacgc ccgcatccgc
     2941 actgtccagc ggccgcacgc gcaccggttg gctacggtct actttgccca gatcggcgac
     3001 gggcagcgga acgcgcaccc agactccccc ggcgccgggc ttggtcgtcg tatccggcgg
     3061 cccggcatcg ggcaggctca cggccccgcc cacgctccgc ttccgtatga ccggctcctc
     3121 gcgcaccggc gccgtctgga tctttagccg ttgcatcgcc tgctccgaaa gcaccacgtt
     3181 atggaggtcg ctcccggcga tcggggtgat gccggcgggc ggg
//
LOCUS       DASUYC010000450         2462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1782686, whole genome shotgun sequence.
ACCESSION   DASUYC010000450 DASUYC010000000
VERSION     DASUYC010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1782686"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..320)
                     /locus_tag="VF276_07435"
     CDS             complement(24..320)
                     /locus_tag="VF276_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606728.1"
                     /translation="MRTQSPDTTPEAEAVLIAGLQRLGPGRRLQMADDLTRMTRQLSW
                     YGLRRRYPTASEDELRYRWCALLYGEELAAGYIAAWRAHRRTYPAAPADAVGPA"
     gene            complement(400..2058)
                     /locus_tag="VF276_07440"
     CDS             complement(400..2058)
                     /locus_tag="VF276_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007263216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding dehydrogenase"
                     /protein_id="HEX6606729.1"
                     /translation="MSDAADVIVVGAGLAGLVAAAEVAGAGKRVIIVEQEPPQSLGGQ
                     AFWSFGGLFLVDSPEQRRLGVRDSLALARQDWRGTAGFDRPEDYWPRRWADAYVQWAA
                     GEKRAWLHAQGVRFFPIVGWAERGGYNAGGPGNSVPRFHITWGTGPGILAPFVRRVRA
                     AERRGLVRVRFRHRVSELTVEGGVVTGVRGEVLEPSDAPRGQASSRTAVGTFALRAQA
                     VIVASGGIGGNPDLVRQNWPARLGTPPAHMLAGVPEHVDGRMLGISEAAGAHLINRDR
                     MWHYTEGIQNCDPIWRDHGIRILPGPSSLWLDARGRRLPVPLYPGFDTLGTLAHLMQT
                     GSDYSWFVLTQPILRREFALSGSEQNPDLTGKSVRGILRRAVGKAAPAPVEAFKQHGA
                     DFVVADTLGALVRGMNALTPDPLLDPAAVERQVLARDREITNPFTKDSQIAHIRGARR
                     YLGDRLIRTAKPHRLLDPAAGPLIAVRLHILTRKTLGGLETDLAGRVLRPDGQPLPGL
                     YAAGEAAGFGGGGVHGYRALEGTFLGGCLFSGRAAGRAAAAAVG"
BASE COUNT          359 a          900 c          851 g          352 t
ORIGIN      
        1 gggaatacta tactggggcg gattcatgcc gggccaactg catcggcggg agcagcggga
       61 tacgtacggc ggtgcgcccg ccaagccgcg atatacccgg cggccagttc ctctccgtag
      121 agcagggcgc accagcggta gcgcaattca tcctccgagg cggtaggata gcgccggcgg
      181 aggccatacc aggaaagttg gcgagtcatg cgggtcagat cgtcggccat ttgcagccgg
      241 cggcccggcc caaggcgctg tagcccggca attaaaaccg cttcggcttc cggcgtcgta
      301 tcgggcgatt gggtgcgcat cctgtgcttt cctcctgggc agggcggccc ggcgggaacc
      361 cgcgctttgt atgtacctat tatagcacac ccgttccgcc tacccgaccg cggccgccgc
      421 ggcccggccc gccgcgcggc ccgagaagag gcagccgccc aggaacgtgc cttccagcgc
      481 gcggtaaccg tggacgccgc ccccgccgaa cccggcggcc tcgcccgcgg cgtagaggcc
      541 gggcagcggt tggccgtcgg ggcgtagcac ccggcccgcc aggtccgtct ccaggccgcc
      601 cagcgtcttg cgcgtgagga tgtgcaggcg cacggcgatc agtggcccgg cggcggggtc
      661 gagcaaccgg tgcggcttgg ccgtgcggat cagccggtcg cccaggtagc ggcgcgcgcc
      721 gcggatgtgg gcgatctggc tgtccttggt gaacgggttg gtgatctcgc ggtcgcgggc
      781 cagcacctgg cgctccaccg ccgccgggtc gagcagcggg tcgggggtca gcgcgttcat
      841 gccccgcacc agggcgccca gggtgtcggc gaccacaaag tcggcgccgt gctgcttgaa
      901 agcctcgacc ggggcgggcg ccgccttgcc cacggcgcgg cgcaggatgc cgcgcacgct
      961 tttgccggtc aggtcggggt tttgctccga gccggacagg gcaaactcgc ggcgcaggat
     1021 cggttgcgtc agtacgaacc agctataatc ggagccggtc tgcatcaggt gggccagcgt
     1081 gcccagcgta tcgaagcccg gatagagcgg cacgggcagg cgccggcccc gcgcgtcgag
     1141 ccacagcgac gacgggccgg gcaggatgcg gatgccgtgg tcccgccaga tggggtcgca
     1201 gttctggatg ccctcggtgt agtgccacat gcggtcgcgg ttgatcaggt gcgcgcccgc
     1261 cgcctcgctg atgcccagca tccgcccgtc cacgtgctcc ggcaccccgg ccagcatgtg
     1321 cgcgggcggg gtgcccagcc gggccggcca gttctggcgc accaggtcgg ggttgccgcc
     1381 gatgccgccc gacgcgacga tgaccgcctg cgcccgcagc gcgaacgtgc cgaccgccgt
     1441 gcgcgaactg gcctgcccgc gcggcgcatc gctcggctcc agcacctcgc cgcgcacgcc
     1501 cgtgaccacg ccgccctcga cggtgagctc gctgacgcgg tggcggaagc ggactcgcac
     1561 caacccgcgc cgctccgccg cccgcacgcg ccgcacgaac ggcgccagga tgccggggcc
     1621 ggtgccccag gtgatgtgga agcgcggcac cgagttgccg gggccgcccg cgttatagcc
     1681 gccgcgctcg gcccagccca cgatggggaa gaagcgcacc ccctgggcgt gcagccaggc
     1741 gcgcttctcg cccgccgccc actgcacata ggcgtcggcc cagcggcgcg gccagtagtc
     1801 ttcgggccgg tcgaaccccg ccgtgccgcg ccagtcctgc cgcgccaggg ccagcgagtc
     1861 gcgcaccccc aggcggcgct gctccggcga gtccaccagg aacagcccgc cgaacgacca
     1921 gaacgcctgc ccgcccaggc tctgcggcgg ctcctgctcg acgatgatca cccgcttgcc
     1981 cgcgccggcc acctccgccg cggccaccag tccggccagc cccgccccca cgacaatcac
     2041 atccgccgcg tcgctcatgc tccctcctgt gcgctcccta aaatctgggc gtatcatagg
     2101 gggttcgccc cggcgtgtca agccgtgccc gtctgcgggc cgtataaact caccgcgaag
     2161 gaacgaagga cgcgaagacg atttgtgttt agaaaactgc cctgtgtaga cagcttattt
     2221 gctctgtgct atgataacgc cgtaccgcac ccaatagcta taccgggacc ggcccctgga
     2281 ggccggcgcc gccactgccc agggatgtgc tgatgcctgc ccatcgtgtc cgtcgcgccc
     2341 tatccgaaga tccggcatca ccgattaggg cgcgcctcat tgtcctgctg ataatcttca
     2401 tgggcgtggc gctcgtggcg tgcggcatgg aggccttgcc ggaaaccacc ccgatcagca
     2461 cg
//
LOCUS       DASUYC010000451         3326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1185988, whole genome shotgun sequence.
ACCESSION   DASUYC010000451 DASUYC010000000
VERSION     DASUYC010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185988"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VF276_07445"
     CDS             <1..198
                     /locus_tag="VF276_07445"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019552042.1"
                     /note="Catalyzes two discrete reactions in the de novo
                     synthesis of purines: the cleavage of adenylosuccinate and
                     succinylaminoimidazole carboxamide ribotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX6606730.1"
                     /translation="RKAGHPNPYEKLKELTRGARITEADMRAFIERLDLPPADRARLL
                     ALSPATYTGLAAALVAHIEDE"
     gene            807..992
                     /locus_tag="VF276_07450"
     CDS             807..992
                     /locus_tag="VF276_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606731.1"
                     /translation="MSVETDEFLHGLFGYDEDSDEGPYVNWTNGFEASDEEYEEYTAQ
                     DNNNNNSGGWWRFWDKD"
     gene            complement(1102..2994)
                     /locus_tag="VF276_07455"
     CDS             complement(1102..2994)
                     /locus_tag="VF276_07455"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX6606732.1"
                     /translation="MPTIVTSGTRQNGAPEASGGVALLQVGAAMDLGLRATQEDALGV
                     SLPAEDEAARRGGLFVLADGLGGHTAGELASRLAVQTIGAAFYASSMRQPGNLLQSAI
                     EQANRRLLAAGQDPALRGLGSTCVCAAVQGDSLWVANVGDSRAYLMRPPGTLQQLSQD
                     HSMAAEQVRQGLIASEDHADHAMRSVLLQSLGVPSGVQPYFAGPLPLQKGDLLLLCSD
                     GLYNALDERAMHDYLLASLLSTLTVSADQIARDLVEGARFHGGDDNISALVVYCTDVV
                     PEAVARAAGWPQSQARSLFLPMNGAAAQRAPDAPAPHAAPAAEEVSAGLPASPAPAAG
                     DESRGPAGPPADLPYLPAVPTYTRTRIPALSLAALIGGGVLFLLLALAWGVAAFWAPR
                     WLVPIFLTMLALAIPLGGLLFLNRPRLVPVPVPTVEPGPPLDDDRAAVARAATEPSRS
                     PVLFDDPVAALITRLLPGHAGPVDVDRSLAVPGSGPVAPVIPPDAPPDYVNQAMSAAL
                     GRAHQQWTFNLLMAGVSATLVIGGCLVTIGTLLLGKGEQLGALFGPGVGLLGIVSWLV
                     TQPTAQLNRAGNQISLLTIVWTNYAQELRNCARITDPTQAAACSQRAGAEAVQYFNQI
                     VAEKSS"
     gene            complement(3009..>3326)
                     /locus_tag="VF276_07460"
     CDS             complement(3009..>3326)
                     /locus_tag="VF276_07460"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6606733.1"
                     /translation="DWLGSRYRLVAAGDGAAALAQVTAQPPDALVLDLHLPDMDGAEV
                     LTRLRAMPAGRMIPVVVFSSAAHDVTLVRRLTALSEPGPLLFAAKGAKVDGLLSALRR
                     ALG"
BASE COUNT          567 a         1175 c         1087 g          497 t
ORIGIN      
        1 cgcaaagccg ggcaccccaa cccctacgaa aagctcaaag agctgacccg cggcgcccgc
       61 atcaccgaag cggatatgcg cgccttcatc gagcgcctgg atcttccccc cgccgaccgc
      121 gctcgcctgc tcgcgctcag ccccgcgacc tacaccggcc tggccgccgc tctcgtggcc
      181 cacattgagg acgagtagcc cggtcgccgc cctgtaggga cggtttcaaa ccggacgtac
      241 cccataaccc aacacagaac cggccacgtt ccttctcctc agttatgtca actcaatgcc
      301 gcctctggct taccggagaa cgcctagccg atcggaccac cggcagaccg gctcatcagt
      361 agtcatattt tcaccggtta tccctgagat atagtaagat tttcctattg actcgcgcta
      421 cggagataca atatagtgga ggtatcttgt agtgccggcc gagaaactcg acggcttcgc
      481 cccggcagtg tggcgccggg catacggcga gcgcggcctg tacccattcc cctggtcact
      541 tggcgcccat atggggacga caccctggac tctgggacct gttggccgat ccctcttagc
      601 cgatccgaca ttccatctat ccaccgcgta gatacgccct gcccggatct gcaactttga
      661 tccgggtgca gcgttaagat tgtatagcgc attgcccgac gcgggcacac catataggcg
      721 catcgttggc ggcggcccct gattgatcat cctgatggga gccaccggtg ttggacgatg
      781 gctcccctga cgtgagggag ccggaaatga gcgtggaaac cgacgagttc ctgcatggcc
      841 tgttcggcta tgacgaggac tcggacgaag gaccgtatgt aaactggacc aacggattcg
      901 aggcgagcga cgaggagtac gaagagtaca ccgcgcaaga caacaacaac aataacagcg
      961 gcggttggtg gcgcttctgg gacaaggatt agcgccgctg ggactttgcc acgggcggta
     1021 ggcacccctc gctcgcggca taataacgaa agggcggtca cggagaccgc cctttttgtt
     1081 gccatggcgc gggccgggcg gctaactcga tttttccgcc acgatctggt tgaaatactg
     1141 caccgcttcg gcgcccgcgc gctggctgca cgccgccgcc tgcgtgggat cggtgatccg
     1201 cgcgcagttg cgcagttcct gcgcgtagtt ggtccagacg atggtcagca ggctgatctg
     1261 gttgccggcg cggttcaact gcgcggtggg ctgggtgacc aaccagctca cgatgccgag
     1321 caagcccacg ccggggccga acaacgcgcc caactgctcg cccttgccca gcagcagcgt
     1381 cccgatggtc accaggcagc cgccgatgac cagcgtcgcg ctcactcccg ccatcagcag
     1441 gttgaacgtc cattgctggt gcgcccgccc cagcgcggcg ctcatggcct ggttcacata
     1501 gtcgggcggc gcatcgggcg ggatcaccgg cgccaccggc ccgctaccgg gtacggccag
     1561 gctgcggtcc acgtccaccg gcccggcgtg gcccggcagc agtcgcgtga tcagggctgc
     1621 gaccggatcg tcgaagagca cagggctgcg gctcggttcg gtggccgcgc gggccaccgc
     1681 cgccctgtcg tcgtccagcg gcgggccggg ttcgaccgtt ggtacgggga ccggcaccag
     1741 gcgcgggcgg ttcaggaaca gcagcccgcc cagcggaatc gccagcgcca gcatagtcag
     1801 gaagatcggc acgagccagc ggggcgccca gaacgcggcc acaccccagg ccagcgccag
     1861 cagcaagaac aacacgcccc cgccgatcag cgccgccagg ctcaacgccg ggatgcgcgt
     1921 ccgcgtataa gtgggcacgg cgggcagata cggcagatcg gcaggcggac cggcggggcc
     1981 acggctctcg tccccggctg cgggcgccgg ggatgcgggc agcccggcgg acacctcttc
     2041 agcagcaggc gcggcgtgcg gcgcgggcgc gtccggcgcg cgctgggccg ccgccccgtt
     2101 catcggcaag aacaggctgc gcgcctggct ctgcggccac cccgccgcgc gggccaccgc
     2161 ttcgggcacc acgtcggtgc agtagacgac cagcgccgag atgttgtcgt cgccgccgtg
     2221 gaagcgcgcg ccttcgacca ggtcgcgggc gatctggtcg gcgctcacgg tgagcgtgct
     2281 caagagcgag gccagcagat aatcgtgcat ggcccgctcg tccagcgcat tgtagaggcc
     2341 atccgagcag agcagcagca gatcgccctt ttgcaagggc agcggcccgg cgaagtaggg
     2401 ctgcacgccg ctgggcaccc ccagcgattg cagcagcacc gagcgcatgg cgtggtcggc
     2461 atggtcttcg ctggcgatca gaccctggcg cacctgttcg gcggccatcg aatggtcctg
     2521 cgaaagctgc tgtagcgtgc cgggcggacg catgagatag gcccggctgt cgcccacgtt
     2581 ggcgacccag aggctgtcgc cctgcaccgc cgcgcagacg caggtgctgc ccagcccgcg
     2641 cagcgccggg tcctgcccgg cggccagcag gcggcggttg gcctgctcga tggcgctctg
     2701 caacaggttg cccggctggc gcatcgacga cgcatagaag gccgcgccga tggtctgcac
     2761 ggccaaccgg ctcgccagtt ccccggcggt atgcccgccc aggccgtcgg ccagcacaaa
     2821 cagcccgccc cggcgcgcgg cctcgtcctc cgcgggcaag gacacgccca gcgcgtcttc
     2881 ctgcgtggcg cgcaggccca ggtccatcgc cgcccccacc tggagcagcg ccacgccgcc
     2941 cgaagcttcc ggcgccccgt tttgccgggt gcccgatgtg acgattgtcg gcatcttgtc
     3001 gttccttcct agcccagagc gcgccgcagc gcgctgagca ggccgtccac cttcgcgcct
     3061 ttcgccgcga agaggagcgg tccgggttcc gagagcgcag tcaaccgccg tacaagcgtc
     3121 acatcatgtg cggcactgga aaacaccacc accgggatca tccgcccggc gggcatcgcg
     3181 cgcaagcggg tcaagacctc ggcgccatcc atatccggca ggtgcaaatc aagcaccagc
     3241 gcatcgggcg gctgcgccgt cacctgggcc agggccgccg cgccgtcgcc cgcggcaacc
     3301 agccggtagc gcgaccccag ccagtc
//
LOCUS       DASUYC010000452         2058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_250343,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000452 DASUYC010000000
VERSION     DASUYC010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250343"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VF276_07465"
     CDS             <1..280
                     /locus_tag="VF276_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606734.1"
                     /translation="NATGGVAGGPVMIGAIAAATADEKNILGGDQDDPYMPDPGSRAA
                     RENREGATMGQLGDTNVEAGGRTGTGTETFGERQARADDDTEGYWRIG"
     gene            305..1447
                     /gene="murG"
                     /locus_tag="VF276_07470"
     CDS             305..1447
                     /gene="murG"
                     /locus_tag="VF276_07470"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEX6606735.1"
                     /translation="MRILITGGGTGGHVSPAVAVIACLRARSRAEGWPLDLLYLGSRD
                     GVERRIIGELGVPYAAVQTGKLRRYFSWRTPLDLARIPIGVIQSIWQVARFRPAVIFS
                     TGGYVCVPPVLAGRLLGVPAITHEQTVRSGLANRIASRFAQVVAISFPESAAEFPPGR
                     TVLTGNPLRSELHGGDAARAAEYWHFDPAIPVIYVTGGAQGAHVINTAVRAALPQLLA
                     VAQVVHQVGEGPEGSRADLRAAEDQAQATPEARARYRPVAYTGPELADLYALARLVIG
                     RAGAGTVNELAALGKAAVLIPLPGTGSDEQTANARRLEATGGAVVLPQAECTPDRLAA
                     LVRDLLAGPDRLAQMAAAGRTLARPDAADALVRLILKVALHAPDTP"
     gene            complement(1454..>2058)
                     /locus_tag="VF276_07475"
     CDS             complement(1454..>2058)
                     /locus_tag="VF276_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606736.1"
                     /translation="EPAWDPRGGLWSLYTEHWFDPTTNAPLYETRLVHIGAEDGQATN
                     IYQAASKTLGRPRSNPAHPEQVAVADGTDLLLLTVDLAADKPVSARRVLRHGVVRNSP
                     VAWSPGGDKLYYVQPGAQDPTTDSLQELDLASGQSREIYVFLPEQQEFSGFDLSPDGQ
                     SFVYETKGDLYTRPLDAPATEPSHHITYFGQASHPTWGGR"
BASE COUNT          289 a          757 c          718 g          294 t
ORIGIN      
        1 caatgccacc ggcggggtcg ccggcgggcc ggtgatgatc ggggccatcg ccgcggcaac
       61 cgccgacgag aagaatatcc tcggcggcga ccaggacgat ccgtatatgc ccgatcccgg
      121 cagccgcgcg gcgcgcgaga accgcgaagg cgcgacgatg ggccagttgg gcgataccaa
      181 cgtcgaagcc ggcgggcgca ccggcaccgg caccgaaacc ttcggcgaac ggcaggcccg
      241 cgccgacgac gacaccgagg gctactggcg tattggctaa cgatccggca gacgagcaag
      301 cacaatgcgt atactgatca ccggcggcgg caccgggggc catgtcagcc ccgccgtcgc
      361 cgtgattgcg tgtctgcgcg cgcgtagccg ggccgaaggc tggccgctcg atttgctcta
      421 tctgggcagc cgcgacggcg tcgaacggcg gatcatcggc gagttgggcg tgccctacgc
      481 cgccgtgcag accggcaaac tgcgccgcta cttctcctgg cgcaccccgc tggacctggc
      541 gcgcatcccc atcggcgtga tccaatcgat ctggcaggtc gcgcgcttcc gccccgccgt
      601 gattttcagc accgggggct atgtctgcgt gccgccggtg ctggccgggc ggctgctcgg
      661 cgtgcccgcg atcacccatg agcaaaccgt gcgctccggc ctcgccaacc gcatcgcgtc
      721 tcgcttcgcc caggtggtgg cgatcagctt cccggaatcc gccgccgagt tcccgccggg
      781 ccggacggtg ctcaccggca accccctgcg gtccgaattg cacggcggcg acgcggcccg
      841 cgccgcggaa tactggcact tcgacccggc catcccggtg atctatgtca cgggcggcgc
      901 gcagggcgcc cacgtcatca ataccgccgt gcgcgccgcc ctgccccaac tgctggccgt
      961 ggcccaggtg gtccaccagg tgggcgaggg gccggagggg tcgcgcgccg atctacgcgc
     1021 cgccgaggac caggcccagg ccacgcccga agcgcgggcg cgctatcgcc ccgttgccta
     1081 caccggcccc gaactggccg atctctatgc tctggcccgg ctggtgatcg ggcgggcggg
     1141 cgcgggcacg gtcaacgaac tggccgcgct aggcaaagcc gccgtgctca ttcccctgcc
     1201 cggcaccggc tccgacgagc aaactgccaa cgcgcgccgg ctcgaagcca cgggcggcgc
     1261 ggtcgtgctc ccgcaggccg aatgcacccc tgaccgcctg gcggctctgg tccgcgacct
     1321 gctggccggc cccgatcgcc tcgcgcagat ggccgccgcc gggcgcaccc tggcccgccc
     1381 ggacgccgcg gacgccttag tgcgcctgat cctcaaggtc gccctgcacg cgccggacac
     1441 cccgtaggtc cggctagcgc ccgccccagg ttgggtggct ggcctgcccg aaataggtga
     1501 tgtggtgcga cggctccgtg gccggcgcgt cgagggggcg cgtgtagagg tcgcccttgg
     1561 tctcatagac gaacgattgg ccgtcgggcg agagatcgaa gccgctgaac tcctgctgct
     1621 ccggcaggaa cacgtagatt tcccggctct ggccgctcgc cagatccagc tcttgcaggc
     1681 tgtcggtggt cgggtcctgc gcgccgggct ggacatagta gagtttgtcg ccgccgggcg
     1741 accaggccac cggcgaatta cgcaccaccc catgccgcag cacccgccgc gcgctgaccg
     1801 gcttatccgc cgccaggtcc accgtcagca gcagcaggtc ggtgccgtcg gcgacggcga
     1861 cctgctccgg gtgggcgggg ttggaccggg gccggcccag cgtcttgctc gccgcctggt
     1921 agatattcgt cgcctggccg tcctcggcac cgatgtggac caggcgcgtc tcatagagcg
     1981 gcgcgttcgt ggtcgggtcg aaccagtgct cggtatagag gctccacagg ccgccgcgcg
     2041 ggtcccaggc cggctcgg
//
LOCUS       DASUYC010000453         3119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_473970,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000453 DASUYC010000000
VERSION     DASUYC010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473970"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..927
                     /locus_tag="VF276_07480"
     CDS             85..927
                     /locus_tag="VF276_07480"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6606737.1"
                     /translation="MPQNSPEETARLRRILPERAIALAMQSRWTEAVDVNKAILAIDP
                     NDADAHNRLGKAHLELGNYREAYEAYKRAVELAPGNAIAQKNMQRLAPLAQQPAGAAA
                     AVAKRPAAQPAERISPHTFIEETGKTGLAHLTDLASGPTLLSLTGGDRVNLVLVGAKL
                     QVQTESGSYLGLVEAKLAHRLIRFMQAGNRYTATVTTVSDRSLTIIIREAYQDPSMRG
                     RQSFPSKGAAAAYRPAATSGRYGYDEDEDLGGEFESENEADVEAEDSEEETEFEDDDI
                     EDRE"
     gene            1006..1416
                     /locus_tag="VF276_07485"
     CDS             1006..1416
                     /locus_tag="VF276_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606738.1"
                     /translation="MYALFPLLIVAIACGMLGAWLVHEAGFVRNPALVLPGRLLIAVL
                     PLVLFGFVAATVSRGLAGQDGGPVLAVLLGAAALLLLLALAGVVRARVQGRRAGPARP
                     GLLGPTLLVPAIGIITACYVLSLLLALTNGTLRF"
     gene            complement(1468..>3119)
                     /locus_tag="VF276_07490"
     CDS             complement(1468..>3119)
                     /locus_tag="VF276_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606739.1"
                     /translation="IQRVVGAGAQVTVILDSCHSGGATRDISTDPAAPAVREIILDPQ
                     KLDWAAFQAEHGLDGNGAGDSATRDIAGGSGWVPSLAQGQELIVLSGCRDVQTSREYT
                     PPGTNERHGALTYFFLDALKTADPARATQLRWQDIYPGIRTNVQTAFPDQTPTLEGLG
                     ERPLFGGTWQPYAPGFTVSVNPGGNSLTLDGGSIHGLGPGAQLAVYPPDTADFSQAAQ
                     VAVAEVDSATPVTSQAHIVGTPTAAVANGARAVLTRPSTATPRLAVKLTDVPDAVVAG
                     IRGLQGIDNFVDLAPANQAPSVEVRPRTGIDGKDGWVLVKYTSNETALGPNDVIAYAP
                     RNMEGVRIDPAKLGEAIGEGLVHWARYWSVLTRHNDEPRLQGAITLSVLAGNDDAALR
                     QAGAQPGGPVRSPDAAGQVVVGQDEVLLLKVNVAPTSPVALNVVIMLCSDDGNIDLLW
                     PPENAENVLPAGEERQVGVNGPNPFQLTIRDDQTASRYVLKLFASDQGHPFATNSLKL
                     EGTVQDEIDGGLRGGATPSLPSVLWTTVDVPVLVTRQRPAK"
BASE COUNT          454 a         1070 c         1091 g          504 t
ORIGIN      
        1 ctgtggtgtg ccccaccgtg gccctctacc aggccgccgg gcgcattaat cgaaacgcat
       61 aataagtgag gtgaggtaac cgcaatgcct cagaatagtc ctgaagaaac cgcgcgcttg
      121 cggcgtattc tgccggaacg ggccattgcc ctggcgatgc agagtcgctg gaccgaagcc
      181 gtggacgtga ataaggcgat cctcgccatc gatcccaacg acgcggacgc ccataaccgc
      241 ctgggcaagg cgcacctgga gttgggcaac taccgggaag cctacgaagc ctacaagcgc
      301 gcggtcgaac tggcgccggg caacgccatc gcgcagaaga atatgcagcg cctggcgccg
      361 ctggcccagc agcccgccgg cgccgccgcg gccgtcgcca agcgcccggc ggcccaaccc
      421 gccgagcgca tcagcccgca taccttcatc gaggagacgg gcaagaccgg cctggcccac
      481 ctgaccgacc tcgccagcgg gccgaccctg ctgtcgctga ccggtggcga ccgggtcaac
      541 ctggtgctgg tcggcgccaa gctgcaggtg caaaccgaaa gcggatccta cctgggcctg
      601 gtcgaggcga agctggcaca ccgcctgatc cgcttcatgc aggcgggcaa ccgctacaca
      661 gccaccgtca ccacggtgag cgaccgcagc ctgacgatca tcatccgcga ggcctaccag
      721 gacccgtcga tgcgcgggcg gcaatcgttc ccctccaagg gcgcggcggc ggcctatcgc
      781 ccggcggcca cctcgggccg ctacggctac gatgaggacg aggacctggg cggcgagttt
      841 gagagcgaga acgaggccga cgtcgaagcc gaggattcgg aagaggaaac cgagttcgag
      901 gacgacgata tcgaggatcg cgagtaaccg gcatacggat acggcacggg gccaggagag
      961 cagccatctc ctggcccttc gcctatccgg cgcggtgtaa gcccgatgta tgctctgttt
     1021 ccgctcttga tcgtcgccat cgcctgcggc atgctcggcg cgtggctggt ccatgaagcc
     1081 ggctttgtgc gcaacccggc gctggtcttg cccggccggt tgctcatcgc cgtgctgccg
     1141 ctggtgctgt tcggctttgt agcggcgacc gtgagtcgtg ggctggccgg gcaggatggc
     1201 gggccggtgc tggcggtgct gctgggcgcc gccgcgctgc tgttgctgct ggcgctggcc
     1261 ggcgtggtcc gcgcccgtgt ccagggccgc cgggcgggcc cggcccgtcc gggcttgctc
     1321 ggccccaccc tgctcgtccc cgctatcggc atcatcactg cgtgctacgt gctgagcctg
     1381 ctgctggccc tgaccaacgg caccctgcgc ttctaagcgc cgccgaatcc cgccggcccc
     1441 tacaacgcgc ggcgggatgg tgcccagcta cttggcgggg cgctgccgcg tgacgagcac
     1501 cggcacgtcc accgtcgtcc agagcacgct gggcagggag ggcgtggcgc cgccgcgcag
     1561 gccgccgtcg atctcgtcct gcaccgtgcc ctccagtttc aggctgttcg tcgcgaacgg
     1621 gtggccctga tccgaggcga acagcttgag cacgtagcgg ctggccgtct ggtcgtcgcg
     1681 gatggtcaac tggaacgggt tggggccgtt cacgcccacc tggcgctctt cgcccgccgg
     1741 cagcacgttt tccgcgttct ccggcggcca cagcaggtcg atgttgccgt cgtcggagca
     1801 gagcatgatc accacgttca gcgcgaccgg actggtgggc gccacgttga ccttgagcag
     1861 cagcacctcg tcctggccca ccaccacctg gcctgccgca tcgggtgagc gaacggggcc
     1921 gcccggctgg gctcccgcct ggcgcagcgc ggcatcgtcg ttgccggcca gcacgctgag
     1981 cgtgatcgcc ccttgcaagc gcggttcgtc gttgtggcgg gtcagcaccg accagtagcg
     2041 cgcccagtgg accaggcctt cgccgatggc ctcgcccaat ttggccgggt cgatgcgcac
     2101 accctccata ttgcgcggcg cataggcgat cacatcgttc ggtcccagcg ccgtttcgtt
     2161 gctggtgtac ttgaccagca cccaaccatc cttgccgtcg ataccagtgc gcgggcgtac
     2221 ctccaccgac ggcgcctggt tggcgggcgc caggtccacg aagttgtcga tgccctgcaa
     2281 gccgcggatg ccggcgacca cggcatcggg cacatcggtc agcttgaccg ccagccgtgg
     2341 cgtggccgtg ctgggccggg tcaggacggc ccgcgcgccg ttggcgaccg cggcggtggg
     2401 cgtgccgacg atgtgcgcct ggctcgtcac cggggtggcg ctgtcgactt cggccacggc
     2461 aacctgggcg gcctggctga agtcggcggt atcaggggga tagacggcga gctgcgcgcc
     2521 tgggcccagg ccgtggatgc tgccgccgtc cagggtcagg ctgttgccgc cggggttcac
     2581 gctgacggtg aagccgggcg cgtagggctg ccaggtgccg ccgaacaggg gccgctctcc
     2641 tagcccttcc agcgtggggg tctggtcggg gaaggcggtt tgcacgttcg tgcgtatacc
     2701 ggggtagata tcctgccagc gcagttgggt cgctcgcgct gggtcggcgg tcttcagagc
     2761 atcgaggaag aaataggtca gcgcgccgtg cctttcgttg gtgccgggcg gcgtgtattc
     2821 gcgcgaggtc tgcacgtcgc ggcacccgga gagtacgatc aactcctggc cctgggcgag
     2881 gctgggcacc cagccgctgc cgcccgcaat atcgcgcgtg gcgctgtccc ccgcgccatt
     2941 gccgtccagc ccatgttccg cctggaaggc ggcccagtcc agtttctgcg gatcgaggat
     3001 gatctcgcgc acggcggggg ccgccgggtc ggtgctgatg tcgcgggtcg ccccgcccga
     3061 gtggcacgag tcgaggatga cagtgacctg ggcgcccgct cccaccacgc gctggatgc
//
LOCUS       DASUYC010000454         4980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_1835,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000454 DASUYC010000000
VERSION     DASUYC010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4980
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1835"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..439)
                     /locus_tag="VF276_07495"
     CDS             complement(194..439)
                     /locus_tag="VF276_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015154379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin family protein"
                     /protein_id="HEX6606740.1"
                     /translation="MSKTVSALFDGQVLRFDEPLDLEPNTRYKITIEPETDDVRDADA
                     WDVLERFAGSVDAPPYWASEHDHYLYGTPKRQAENSL"
     gene            complement(523..1020)
                     /locus_tag="VF276_07500"
     CDS             complement(523..1020)
                     /locus_tag="VF276_07500"
                     /inference="COORDINATES: protein motif:HMM:NF024635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6606741.1"
                     /translation="MKRVLLTGMSGTGKSTLTGALAARGYKAVDADTDEWSHWVAVSG
                     PDSAGPPVRPDEDWVWREDRIQTLLATEDSDILFMSGCAENMVQFLPQFDHVVLLSAP
                     ASVIAERLANRTTNDYGKQPDEVARVLDQQHTVEPLLRRAAGHEIDTSAPLEQVLATV
                     LQIVL"
     gene            complement(1112..1924)
                     /locus_tag="VF276_07505"
     CDS             complement(1112..1924)
                     /locus_tag="VF276_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606742.1"
                     /translation="MGFLDQVKQSGQRAAWEAERMVRVNKANSAVADAKRDLDSQIYN
                     LGKTMIAAFEGGATPSAEAQPVYDQIKALEDKVKQLEQEVERIKQEQPPGTAPQPAPP
                     PGYPSTAQGAPYPAPGGGAPYTAPNQGAPYTPTGPTAGPGPYTPTGPTAGPGPYTPTG
                     PTAGPGPAVASGPYTPTGPAAPGPYVPTGPSPETPSGPGATGATAYGAPAYEQAAAPT
                     SHDAAPGMAPGAPASEPGAAPSVPAKVFCQNCGTERTDPTAAFCQECGTRYS"
     gene            complement(2123..4096)
                     /locus_tag="VF276_07510"
     CDS             complement(2123..4096)
                     /locus_tag="VF276_07510"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HEX6606743.1"
                     /translation="MAVAHTEIEAPPMIAARRERVAIGLGALVVALGSAVLYLRTLGA
                     GLGGTIDSAEFQHAAYNLSIVHPTGYPMYLLLGRLWLTLLPLGEVAWRMNLLSAVFAV
                     AALLVVYATVVYLGRSVVGGVFAAALLGTHALVWETAVVAEVNSLNLLLVGASLYALL
                     RWAGVGAGRRWPLEAAALLYGLALAHHRTSLLLAPAVAIFVVWMIRQGTRVSPATVLR
                     CVALLLLPWLFYLYLPLRAATTPWYTNTWANFSAEVGGASAWPVILDTIKRPLLPRVG
                     LVWGQVFPRLIGLSTAGLAGAGLLLWARWPQAARRPGVTAPVLVLYGLGFLTICGVMV
                     VYDVDVIGDYLALAVLIAATWAGVAAGWLLGAVRDIPRLQWRGLPAGVLVAGLVLLAL
                     PAAQVRANYAAADFSHFDERALWTTLAAADGTPQALPQDAILIGGWAHYNELRYRQTV
                     EGWRPDLMPVVLDDLVAGGHLNLIDEWLAQGRGVFLVDPTPDITDHFATVPQGPLARV
                     TKREELAAVAMEHPLDITFGDNIHLLGYTLQPAAPRVGGQLRITLFWRTDVRLQERYV
                     VFTHLVDEAVHKVGQKDDEPVRGYRPTVNWAPGETVVDTLVMPIDPAAAPGTYRLVVG
                     LYTRFGERRLVAHDATGQNLGDYWEMTPVRVTR"
     gene            complement(4375..>4980)
                     /locus_tag="VF276_07515"
     CDS             complement(4375..>4980)
                     /locus_tag="VF276_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX6606744.1"
                     /translation="PTATPAPPAPPTVPPEPSGRFFPETGQTVGGPFLAYWLIHGGLA
                     QQGYPLSGEFAEKSDTDGKTYTVQYFERAVFEQHPENQPPFDVLLSLLGVFRYHDKYP
                     AGAPNQQPNTAPGSVLFAETGHRVGGVFLDYWRSHGGLAQQGYPISDEFMERSDLDGK
                     VYRVQYFERAVFELHPEHAPPYNVLLSQLGTYRYHARYPGR"
BASE COUNT          782 a         1657 c         1752 g          789 t
ORIGIN      
        1 tattcgttcg caactaccat aacctcttta gcttgtatca gcgagaggcg aaatacatgt
       61 ctggtgagtc ggatacaatc tatatcactc aatccctgtg ctttggctgt cctgtaaaaa
      121 tcagtgggat ttaattgttc accatcaaga gaaacttcat tcctaatacc aggcatagtg
      181 aatcgtcctt cactcacagg gaattctcag cctgccgctt cggtgtcccg tagagataat
      241 ggtcgtgttc gctggcccag tacggcggcg catccacgct cccggcgaag cgttccagca
      301 catcccaggc gtcggcatcg cgaacgtcgt ccgtctccgg ctcgatggtg attttatagc
      361 gggtattggg ttcgagatcc agcggttcat caaagcgcaa aacctggccg tcgaacagtg
      421 cgcttacagt cttgctcata ctcgaatgtc tcccctattc gagattcccg tccagaagtc
      481 cgtcatccca ttataacagt attctggtct gttcagggcg cctcacaaaa cgatctggag
      541 gacggttgcc aagacctgct ccagcggcgc gctggtgtcg atctcgtgcc cggcggcccg
      601 gcgcagcagc ggctcgacgg tatgttgctg atccagcacc cgcgcgactt cgtcgggctg
      661 tttgccgtaa tcgttggtcg tccggttcgc cagccgctcc gcgatcacgc tggccggcgc
      721 actcagcagc acgacgtggt caaactgcgg gagaaattgc accatgttct ccgcgcaccc
      781 gctcatgaac agtatgtcgg agtcctccgt ggctaagagc gtttgaatgc ggtcctcgcg
      841 ccagacccag tcctcgtccg ggcgcacggg cgggccggca ctatcggggc cgctgaccgc
      901 gacccagtgg gaccactcgt ccgtgtccgc atcgacggct ttgtagccgc gggcggcaag
      961 cgcgccggtg agcgtggact tgccggtgcc cgacatcccc gtcagaagca ctcttttcat
     1021 accgtcacca tagcctgaga caatgtcgtc gatctcaaaa cagagcagcg cccacgtggg
     1081 cgctgctccg ctgcatagcc gccgagagga tctagctgta gcgcgtgccg cactcctggc
     1141 agaacgcggc cgtggggtcg gtgcgctcgg tgccgcagtt ctggcagaac accttggccg
     1201 gcacactggg cgccgcgccc ggctccgagg ccggcgcgcc gggcgccata ccgggcgcgg
     1261 cgtcatggct ggtgggcgcg gcggcctgct cgtaagccgg ggcgccgtag gcggtcgcgc
     1321 cggtggcgcc ggggccggac ggcgtctccg gcgacgggcc ggtgggcacg taggggccgg
     1381 gggctgccgg gccggtcggc gtgtagggac cggaggccac ggcggggccg ggaccggcgg
     1441 tcgggccggt cggcgtgtag ggaccgggac cggcggtcgg gccggtcggc gtgtacgggc
     1501 cgggacccgc cgtagggccg gtcggcgtgt acggggcgcc ctggttgggc gcggtgtacg
     1561 gcgcgccgcc accgggcgcc ggatagggcg cgccctgcgc tgtgctggga tagccgggcg
     1621 gcggcgcggg ctggggcgcc gtgccgggcg gctgctcctg cttgatgcgc tccacctctt
     1681 gttcgagctg cttgactttg tcttccagtg ccttgatctg gtcgtagacc ggctgcgcct
     1741 cggcgctggg tgtggccccg ccctcaaagg cggcgatcat ggttttgccg agattgtaga
     1801 tctgcgagtc gaggtcgcgc ttggcgtcgg cgaccgcgct gttagccttg ttgacgcgca
     1861 ccatgcgctc ggcctcccag gccgcgcgct ggccgctttg ttttacctgg tcgagaaatc
     1921 ccatgccggt atcctcctct gcggtgatcg gcggatggtc caactacccc gcccattgta
     1981 gcgcagcccg caaagcggcg tcaaggcgcg ataatcggcg tgcatccgga aattcaccgc
     2041 gaagacgcga agaacgcgaa gacgatttat gttagttttt cttcgcgccc ttcgcgcctt
     2101 cgcggtgaga aaaatttaga gatcaacggg tgacgcggac gggggtcatt tcccagtagt
     2161 cgcccaggtt ctggcccgtg gcgtcgtggg cgacgaggcg gcgctcgccg aagcgggtgt
     2221 agaggccgac gaccaaccgg taggtgccgg gcgcggcggc ggggtcgatg ggcatgacca
     2281 gcgtatcgac caccgtctcg cccggcgccc agttgaccgt gggccggtag ccgcgcaccg
     2341 gctcgtcgtc cttctgcccg accttgtgca ctgcttcgtc caccaggtgg gtgaagacga
     2401 cgtagcgctc ctgcaggcgc acatccgtcc gccagaacag ggtgatgcgt aactgcccac
     2461 ccacccgcgg cgcggcgggt tgcagcgtgt agcccaacag gtggatgttg tcgccgaagg
     2521 tgatgtccag cggatgctcc atggcaacag cggccaattc ctcgcgcttg gtgacccgcg
     2581 ccagcggacc ttgcggcacc gtggcgaaat ggtcggtgat gtcgggcgtg ggatcgacca
     2641 ggaagacgcc gcggccctgg gccagccact cgtcgatcag gttcaggtgg cccccggcga
     2701 cgaggtcgtc cagcacgacg ggcatcaggt cgggccgcca gccctcgacg gtctgccggt
     2761 agcgcaactc gttgtagtgc gcccagccgc cgataaggat ggcgtcctgc ggcagcgcct
     2821 ggggcgtgcc gtccgcggcg gcgagagtag tccagagcgc gcgctcgtcg aaatggctga
     2881 aatcggcggc ggcgtagttg gcgcgcacct gcgcggcggg cagcgccagc agcaccagcc
     2941 cggccaccag caccccggcg ggcaggccgc gccattgcag gcgcgggatg tcgcgcacgg
     3001 cgcccagcag ccacccggcg gccaccccgg cccaggtcgc cgcgatcagc acggccagcg
     3061 cgaggtaatc gccgatcaca tccacgtcgt aaacgaccat gaccccgcag atggtcagga
     3121 agccgaggcc gtagagcacg agcaccggcg ccgtaacgcc cggccgccgc gccgcctgcg
     3181 gccaccgcgc ccagagcagc aggcccgccc ccgccagtcc cgccgtgctc aggccgatca
     3241 gccgggggaa cacctggccc cagacaaggc ccacgcgcgg cagcagcggg cgcttgatgg
     3301 tgtcgaggat gaccggccac gcgctcgccc cgccgacctc ggcgctgaag ttggcccagg
     3361 tgttggtgta ccagggcgtg gtcgccgcgc gcaggggcag gtagaggtag aacagccagg
     3421 gcagcaacag cagcgcgaca caccgcagca ctgtggcggg ggaaacccgc gtgccctgcc
     3481 ggatcatcca cacgacgaag atcgccacgg cgggcgcgag cagcaggctg gtgcggtggt
     3541 gggccagggc caggccatag agcagcgccg ccgcctccag cggccagcgc cgcccggcac
     3601 ccaccccggc ccagcgcagc agcgcataga ggctcgcccc gaccagcagc aggttgaggc
     3661 tgttgacctc ggccaccacc gccgtctccc agaccagcgc gtgggtgccg agcagcgccg
     3721 ccgcgaacac gcccccaacc acgctgcggc ccaggtagac caccgtggcg tagaccacca
     3781 gcagcgccgc cacggcaaag acggcggaca gcaggttcat gcgccaggcc acctcgccca
     3841 gcggcagcag ggtgagccag agccgcccta gcagcaggta catcgggtag ccggtggggt
     3901 ggacgatgga gaggttgtag gcggcgtgct ggaactccgc cgagtcgatg gtgccgccga
     3961 ggcccgcgcc gagggtgcgc aggtagagga ccgcgctgcc gagcgccacg accagggctc
     4021 ccagcccgat cgccacgcgc tcccggcgcg ctgcgatcat gggcggcgct tctatctccg
     4081 tgtgcgcgac ggccatgcct cccctttcgc cgccgggggt ccatctcccg gcagcctact
     4141 cccagcggga gtataccgaa agggccgggc cagcggcaag cggggccgcc cggcaagtgg
     4201 gcttgtcatg cttcgctgcg ctcaacatct ccggcaaacg agacgagact cttcgctccg
     4261 ctcagagtga caaggagccg gccccgtcta tcgtctcagc ccgcgaaggc gggcttcgtc
     4321 gcggtagccg gcggcttgag ccgccgggcg ctgccgcggg ccgggcgccc cgcgctaccg
     4381 ccccggatag cgggcatggt agcggtaggt gccgagttgg gagaggagga cgttgtaggg
     4441 cggggcgtgc tccgggtgca actcgaacac ggcgcgctcg aagtattgca cgcgatagac
     4501 cttgccgtcc aggtcgctcc gctccatgaa ctcgtcggag atgggatagc cttgctgggc
     4561 gaggccgccg tggctgcgcc agtagtcgag gaagacgccg cccacgcggt ggcccgtctc
     4621 cgcgaagagc accgatcccg gcgcggtgtt gggctgctgg ttgggcgcgc ccgccgggta
     4681 cttgtcgtgg tagcggaaga cgccgagcag cgagagcagc acgtcaaagg gcggctggtt
     4741 ctcggggtgc tgctcgaaca cggcgcgctc gaaatattgc accgtgtagg tcttgccgtc
     4801 ggtgtcgctc ttttcggcga actcgccgga gagcgggtag ccctgctggg ccagcccccc
     4861 gtggatcagc cagtaggcca ggaaggggcc gccgaccgtc tgcccggtct cggggaagaa
     4921 gcggccgctc ggctccggcg gcacggtggg cggggcgggc ggcgcgggcg tggccgtggg
//
LOCUS       DASUYC010000455         3530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1031789, whole genome shotgun sequence.
ACCESSION   DASUYC010000455 DASUYC010000000
VERSION     DASUYC010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031789"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1988)
                     /locus_tag="VF276_07520"
     CDS             complement(<1..1988)
                     /locus_tag="VF276_07520"
                     /inference="COORDINATES: protein motif:HMM:NF015390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HEX6606745.1"
                     /translation="MPLTAPGFPTQAATLPVVDDFESGLPSGHDANNIAIGFNTFQDP
                     NPATSVAILTSTAPPAPVPGAGNPNTVLQMNLNVVSYAGVTHSFENATLDQWISQDWS
                     AYAGISFWLYGNNSGTTLFVDVLDNRNPGATTDDAERWSIDLPDNFSGWQQIQIPFAS
                     MHRKEIGNGAPNDGFGLTEVHGWALGAVTTPSPQTYYVDDVALYGTAPPRPLTVGFSA
                     ISYPVTEGGTATVTAKLSKPAADPVTVQYRTTIGTAIANRDYTPVAGTLTFPPNVTQQ
                     SFTVTTFDNAKYQGERGVEVELSNPTGGAALGLPPVARVTIRDNETYDPTLLDDFETY
                     PYLWSVANNAALSNPEIPAGAPLALPGQGAYEHILQAAQQQGQGAYTFGHTFPIGQDW
                     SDSGGLNFWYYGRNSGKNIGVTLANDQAGVGDPAQWSLVWHDEFSGQAGAAADTSVWG
                     HEVGDGTANGNPGWGNDELEYYTAGTGNAATDGRGNLAITTKAADGSLQCYYGPCRYT
                     SARLLTKNQFEIAYGRVEARIKVPRGAGLWPAFWMLGTNIDQVNWPQSGEIDIMENVG
                     RLPNQVFGTLHGPGYSGGQSYGRTVDLGTPVADAFHTFAVEWQPNKIVWYLDGAPYFT
                     ATPNDSFLQGKQWVFNHPFFMLLNVAVGGNFGGAVGGDT"
     gene            complement(2416..2853)
                     /locus_tag="VF276_07525"
     CDS             complement(2416..2853)
                     /locus_tag="VF276_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007357065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6606746.1"
                     /translation="MQTILLVEDNELNQDMLRRWLQHWGFQVLLADDGAAGLTLARTA
                     HPDLIVMDINLPVMDGLEATTRLKAVPSTRGIPILVLTAHASIDDRRRSEAAGADDYE
                     TKPVDFGRLRQKILALLAARAAPEGDCIQDVESSGTNRGLNLG"
     gene            3200..3352
                     /locus_tag="VF276_07530"
     CDS             3200..3352
                     /locus_tag="VF276_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005540900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEX6606747.1"
                     /translation="MRKLNDLRKKGQGLVEYALILVLIAIVVIIILALLGGAVNNIFS
                     TIASAL"
BASE COUNT          600 a         1102 c         1160 g          668 t
ORIGIN      
        1 gtgtccccgc cgaccgcgcc gccgaagttg ccgccgacgg cgacgttcag cagcataaag
       61 aacgggtgat tgaagaccca ctgcttgccc tggaggaacg agtcgttcgg ggtcgcggtg
      121 aaatacggtg caccgtccag ataccagacg attttgttcg gctgccactc gaccgcgaac
      181 gtgtggaagg catccgcgac gggcgtaccg aggtccaccg tgcggccgta gctctgcccg
      241 cccgagtagc cggggccgtg tagggtgccg aatacctgat tcggcaggcg gccgacgttc
      301 tccatgatat cgatctcgcc gctctgcggc cagttgacct gatcgatatt ggtgccgagc
      361 atccagaagg ccggccacag ccccgcgccg cgcggcactt tgatgcgcgc ctcgacccgg
      421 ccgtaggcta tctcaaactg gttcttggtc agcaaccgcg ccgaggtgta ccggcacggg
      481 ccgtagtagc attgcagcga cccatcggcg gctttggttg tgatcgccag gttgccacgg
      541 ccgtcggtgg ccgcgttgcc ggtgccggca gtgtagtatt ccagttcgtc gttgccccag
      601 cccggattgc cgttggccgt gccgtcgccg acctcgtgcc cccagacgct ggtgtctgcc
      661 gcggcgcctg cctggccgct gaactcgtcg tgccagacca gcgaccactg cgccgggtcc
      721 ccgacaccgg cctggtcatt ggcgagcgtc acgccgatgt tcttgccgct gttccggccg
      781 tagtaccaga agttcagccc gccggaatcg ctccaatcct ggccgatcgg gaaggtgtgg
      841 ccgaatgtat aggcgccctg gccctgttgc tgcgcggcct gcaagatgtg ttcataggcg
      901 ccctggccag gcagcgccag cggggcgccg gctggaatct ccggattgct cagggccgca
      961 ttgttggcga ccgaccagag ataggggtag gtctcgaagt cgtcaagcag ggtcgggtcg
     1021 taggtctcgt tgtcgcggat ggtgacgcgc gccaccgggg gcagacccag cgccgccccg
     1081 ccggtcgggt tggacagttc gacctcgacg ccgcgctcac cctggtactt ggcgttgtcg
     1141 aaggtggtga cggtgaacga ttgctgcgtg acgttcggcg ggaaggtcag ggtgccggcg
     1201 accggcgtgt agtcgcgatt ggcgatggcg gtaccgatgg tggtccggta ctgcaccgtc
     1261 accgggtcag cggcgggctt gctgagcttg gcggtgactg tcgccgtgcc gccctcggtc
     1321 acagggtagg agatcgcgct aaagccgacc gtgagcgggc gcggcggcgc ggtgccgtag
     1381 agcgccacgt catcgacgta gtaggtctgc gggctcggcg tggtgacggc gcccagcgcc
     1441 cagccatgca cctcggtcag cccgaagccg tcgttgggcg cgccattgcc gatctccttg
     1501 cggtgcatgc tggcgaatgg gatctggatc tgttgccagc cactgaagtt gtcgggcagg
     1561 tcaatggacc agcgctcggc atcgtccgtc gttgcgccgg ggttgcggtt gtcgagcaca
     1621 tccacaaaca aggtggtgcc gctgttgttg ccgtagagcc agaacgagat gcccgcgtag
     1681 gcgctccagt cctgcgagat ccactgatcg agcgtggcgt tctcaaagct atgggtgact
     1741 ccggcatacg agacgacatt gagattcatt tgcaatacgg tgttggggtt accggcaccc
     1801 ggcaccggcg ccggcggcgc ggtgctggtc aggatcgcga cgctggtggc cgggttcgga
     1861 tcctggaagg tattgaaccc gatcgcgatg ttgttggcgt cgtgcccgga cggcagcccg
     1921 gattcaaagt cgtcgacgac gggcaacgtg gctgcctgcg tcggaaagcc gggtgcggtc
     1981 aggggcatgc tgataagcag gctgacaaat aaagtgataa accggaacca tgatcgtcgt
     2041 cttgacatgg tgatctcccc ctggtgatgc gttgctggat ggaccggtca gctacagtag
     2101 cgagggcgcc cgccgggcgc cggcagcacg cagggcggcc tgcaagcagg taacctccat
     2161 ctgaaaggcc gcatgggcgc tgcgccggtg cgccagatcc tcatcgactg tcttaagctt
     2221 attgtaacag gtttgccggc ggattggggc taatttgcac agtgatgtaa agagcatgat
     2281 cgagtcttcg ccaggtggag acggcggtag ccgggcttcg tatggtgcag cctacatcgg
     2341 gggccaacgc ttcgcccggc aattgcagcg atggacgccg ctagatcccc atccgtcgca
     2401 gatttccttg cctgctcacc ccagattcaa acctcggttc gtaccgctgc tttcgacatc
     2461 ctggatacag tccccctccg gcgccgcgcg cgccgccagc agcgcgagga tcttctggcg
     2521 cagccggccg aaatccaccg gcttggtttc gtagtcgtcg gcgcccgcgg cctcgctgcg
     2581 ccggcgatcg tcaatgctcg cgtgcgcggt gagaacgaga atcgggatgc cgcgcgtgga
     2641 ggggaccgcc ttgagccggg tggtggcctc caggccgtcc atgacgggca ggttgatatc
     2701 catcacaatc aggtcgggat gggcggtgcg ggccagggtc aaacccgcgg ccccgtcgtc
     2761 cgccagcagc acctggaagc cccaatgctg cagccagcgc cgcagcatat cctggttcag
     2821 ttcgttatct tcgaccagca agatcgtctg catgatgtgc cttccgtgag atctccgcca
     2881 cccggcgatt gcggcagcgg actgtcgcct ggcgtggcta ggtgccggga gcgcaattcc
     2941 gcgaccatgg aggccgcttt ctgcaagtaa gatactacac ccgcactgac gaaccactga
     3001 caaggccgcc ggcctgctcg gggccgggct acgcggccta ccagggaagc cggatgggta
     3061 ctgaagttcc tatatgaaac aggaccatta ggccatagcc atccaccggg aggatcgact
     3121 acaataatcc tcgtgaacgc tacaccacac cagtgtggcc ccttgaattc agtcttcatc
     3181 aatcactcgg aggaatacaa tgcgcaagct caacgatctg cggaaaaagg gccagggcct
     3241 cgtcgaatac gctctgattc tcgtcctgat tgccatcgtc gtgatcatca tcctggccct
     3301 gctcggcggc gctgtcaaca acatcttctc caccatcgca agcgccctct aagacgcccg
     3361 cgcggccccg gccgcaacag cgtcctctag aaaagggacc cctggtcaca atgaccgggg
     3421 gtcctttttt gcgttgtgcc ggatctgcta taatacagat gcccgttcgc cgatacggcc
     3481 gtagccggcg tgcatccagg agaccgcgcc gttgccctcc ccggttgcca
//
LOCUS       DASUYC010000456         3580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_925156,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000456 DASUYC010000000
VERSION     DASUYC010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925156"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..134)
                     /locus_tag="VF276_07535"
     CDS             complement(<1..134)
                     /locus_tag="VF276_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HEX6606748.1"
                     /translation="METLVLALDLVGTFVFALSGGMRGVRHRLDVFGVFVLSFAAGNA
                     G"
     gene            complement(235..885)
                     /locus_tag="VF276_07540"
     CDS             complement(235..885)
                     /locus_tag="VF276_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606749.1"
                     /translation="MRQPQARAGRHRRLPATGDWRGPPATTATNARHTGNDGLRCGRM
                     RGVPACGLLAQTTLNVAPTMAPDRGVARIVGAAILLLPTYVRRGTYKIPAIGAFAHFG
                     SGRAFYAPPSTDAAIANLPDAPAGSASGSYKMAASRGGSCGGAVSAVIVPARSGDHAP
                     GDGIAGGITCAGWPDHRMISAAARFAVGANLLPIGAAISAILLTVPKGRLHEETRT"
     gene            complement(882..2222)
                     /locus_tag="VF276_07545"
     CDS             complement(882..2222)
                     /locus_tag="VF276_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003929944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX6606750.1"
                     /translation="MASKEDRATTSAASTTAAASAAAVRRVLAGQDAIRPWQEDVYRA
                     IHQHPELSNQEVHTAAVAADTLRKAGYEVHDNVGTTGVVGILRNGAGPIVLMRADMDA
                     LPMQEETGLPYASTDRQTDSAGQVVPVAHSCGHDVHVASLMGAARLLAAHRSAWQGTF
                     IPLFQPAEELGTGAADMVNGGLARLIPKPDVALAQHVLAYPAGTVGTHAGPFLSTAAS
                     MRVTVHGRGSHGSMPQLAIDPVVLAAMIVVRLQAIVAREVAPDDFAVLTVGQIAAGTK
                     SNIIPDHAVIELNMRAYSDSMRTQLIAAIQRAVKGECAASGAPQAPEFALYDEYPLTA
                     NNPAVTAQVAAAFKDYFGDRAFTAPRQSASEDFSRIPDALGVPYTFWALGGIDATQWH
                     KAEAAGRIAGDIPANHSPQFAPVIEPTLQTGTAAIAVAALAWLAAPAAAPGVRP"
     gene            complement(2647..3078)
                     /locus_tag="VF276_07550"
     CDS             complement(2647..3078)
                     /locus_tag="VF276_07550"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX6606751.1"
                     /translation="MPVLLGTGAGGFGAATNYPVGGTPQHMAVGDFNGAGFFDLVTAN
                     FTNNNTSVLLGNGSGGFGATTDFLANTPRTGVAVGDFDRDGSTDLVTTNFYADNESVL
                     LNTCSPPTPTPTNTPTPPTTPTRPNTPTNTPTNTPTNTPAP"
     gene            complement(3316..>3580)
                     /locus_tag="VF276_07555"
     CDS             complement(3316..>3580)
                     /locus_tag="VF276_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606752.1"
                     /translation="VARYLVGTAGLTGARGHPISAARLLGAAYAILRINPDQIHPASH
                     MVWDRIEATVRSQVDENVFAARRAEGEALTREQALAYALADEP"
BASE COUNT          469 a         1262 c         1293 g          556 t
ORIGIN      
        1 ccggcgttgc ccgcggcgaa ggacagcacg aacaccccga acacgtcgag gcgatgccga
       61 acgcccctca tgccgccgct gagcgcgaac acgaaggtgc cgacgaggtc gagtgctagg
      121 acgagcgttt ccatggtgct ctcgggccgc cgggtgcggc cttctcaggc cgagcgtgcg
      181 gtccgcgggg ccggcgcggg cctgcccggc tcccgtccag caggtgcccg gcttctatgt
      241 gcgtgtctcc tcatgcagcc ggccctttgg caccgtcagc aggatggcgc tgatcgccgc
      301 cccgatcggc aggagatttg cgccaacagc gaaccgcgcc gctgcggata tcatgcggtg
      361 gtccggccac ccggcgcagg tgatcccccc cgcaatccca tcccccggcg cgtggtcccc
      421 gctacgcgcc gggacgatca cggccgagac ggccccgccg caggacccgc cacgcgatgc
      481 agccatcttg tagctgcccg atgcggaccc ggccggggca tccggcaggt tcgcgatcgc
      541 tgcgtccgtg gacggcgggg cgtagaatgc gcgcccgctg ccgaagtgcg cgaatgcccc
      601 gatagccggg atcttgtagg taccgcgccg gacgtaggtg ggcagcagca ggatcgcggc
      661 tcccacgatc cgcgcgaccc cccgatccgg cgccatggtg ggtgcgacgt tcagggtcgt
      721 ctgggccaac agcccgcagg ccggcacgcc gcgcatccgc ccgcagcgga gtccatcgtt
      781 accggtgtgg cgcgcgttcg tcgccgtcgt cgccggcggc ccccgccaat cgcccgttgc
      841 cggcaggcgg cggtgcctgc cggctcgggc ctgtggctgc ctcatggccg cactcctggg
      901 gcggcggccg gggccgccag ccaggccagg gcggccaccg cgatggccgc ggttcccgtc
      961 tgcaaggtcg gctcgatgac cggggcaaac tgcggcgagt gattggccgg aatgtcgccg
     1021 gcgatccgcc ccgccgcctc cgccttgtgc cactgcgtgg catcgatgcc gcccagtgcc
     1081 caaaaggtgt acggcacccc gagggcatcc gggatccggc tgaagtcctc acttgccgac
     1141 tgccgggggg ccgtgaaggc ccggtcgcca aagtagtcct tgaaggccgc tgccacctgg
     1201 gccgtgaccg cgggattgtt ggcggtcagc ggatactcgt cgtagagcgc gaactcgggg
     1261 gcttgcggcg cgccggaagc cgcgcactcg cccttgacgg cgcgctggat cgcggcgatc
     1321 agctgggtgc gcatactgtc gctgtaggcg cgcatgttga gctcgatgac ggcgtgatcg
     1381 gggatgatgt tgctcttcgt cccggcggcg atctggccca cggtgaggac ggcaaagtcg
     1441 tccggggcca cctcccgcgc gacgatcgcc tggagacgca cgacgatcat cgcggccagc
     1501 acgacggggt cgatggcgag ctggggcatc gagccgtggc tgccgcgccc atgcaccgtg
     1561 acgcgcatgc tggcggcggt cgaaagaaac gggccggcgt gggtgccgac ggtgcccgcg
     1621 gggtaggcca ggacatgctg ggccagggcg acgtcgggct tggggatcag ccgggccagg
     1681 ccgccgttga ccatgtccgc cgcacccgtg cccagctcct cggccggctg gaacaggggg
     1741 atgaaggtgc cctgccacgc cgatcggtgc gcggcgagca agcgggcggc gcccatcaga
     1801 ctcgccacgt gcacgtcgtg tccgcaggag tgggcgaccg gcacgacctg gcccgcgcta
     1861 tcggtctggc ggtcggtgct ggcgtacgga agacccgtct cctcctgcat cggcagcgcg
     1921 tccatgtcgg cacgcatgag cacgatgggg ccggcgccgt tgcgcaggat cccgacgaca
     1981 ccggtcgtgc cgacgttgtc atgcacctca tacccggcct tgcgcagcgt gtcggcggcc
     2041 acggccgccg tgtgcacctc ctggttggag agctccgggt gctggtggat ggcccgatac
     2101 acgtcttcct gccacggccg gatcgcgtcc tggccggcca gcacgcggcg gacggccgcc
     2161 gctgaggcgg ccgccgtggt gctcgctgct gaagtggtgg cccggtcttc cttgctcgcc
     2221 atgctcgtcg cctccctgga ccgcgctctg ctcaatcggc cgcaagatcg atgccaccca
     2281 tgcccctagc atgcgcgggc tcggcggcgc gctcaccgcc cgcaccgcgg ccgattccgg
     2341 cggggctacg ccgcgccgcg cacccgagag ttggcgcgcg cagggtaggt gccaaatcgt
     2401 ccgcgctgta gctgcctccc gctgcgtccg catcctgggg cggggcgcgg gccagcgcgt
     2461 cgaccgcagg gtcgggatcg gccagcacgc gcgacccagt ctgcgccagg ttctcgcgga
     2521 tcgcggcgcg ccgtcccgcc acgtgccccc gcacggaatc acccctgtgc atgcgtccgt
     2581 tcccccggct gggcggcccc ccgctccccc tggccagggc gccgggtggg gcgggggtcg
     2641 ccgccgttat ggcgcgggcg tgttggttgg cgtgttcgtc ggggtgttgg tcggcgtgtt
     2701 ggggcgcgtc ggagtcgtgg gcggcgtggg cgtattggtt ggcgtcggcg tgggcgggct
     2761 gcacgtattt agcagcaccg actcattgtc ggcgtagaag ttcgtggtga ccaggtcggt
     2821 gctgccgtcg cggtcgaaat cccccaccgc caccccggtg cgcggggtgt tcgccaggaa
     2881 gtcggtggtc gccccgaagc cgccgctgcc attgcccagc agcaccgatg tattgttgtt
     2941 ggtgaagttt gccgtgacca ggtcgaagaa gccagcgccg ttgaaatccc ctaccgccat
     3001 atgctggggg gtgcccccca ccgggtagtt ggtggccgcg ccgaagccgc ccgccccggt
     3061 gcccaggagc accggcacgt tgtttgacca gaagttcgcc gttgccaggt cgtggatcag
     3121 cgcacgccca cccggtaccg atccccgacg gccgccgctt gggtccgaga gtccgcgccc
     3181 cggcggccgg attggatgga ggcgctgctc gatgggcggg cgacagcgct ccgctcgctc
     3241 ctggaggccc acgacctgga atacgcgccg cgtgcccgga tcggtgctaa atacttcagc
     3301 ctccgcccgc cagtactaag gctcgtccgc cagcgcgtag gccagggcct gctcccgggt
     3361 cagcgcctca ccctcggccc gccgcgccgc aaacacattc tcgtctacct gggatcgcac
     3421 ggtggcctcg attcggtccc acaccatatg gctagctgga tggatctggt cagggtttat
     3481 gcggagaata gcgtatgctg cgccgagcag tcgggcggca cttatcgggt gtccacgagc
     3541 accggtcagg cccgcggtgc cgaccaggta gcgcgcgaca
//
LOCUS       DASUYC010000457         4935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1780172, whole genome shotgun sequence.
ACCESSION   DASUYC010000457 DASUYC010000000
VERSION     DASUYC010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1780172"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VF276_07560"
     CDS             <1..639
                     /locus_tag="VF276_07560"
                     /inference="COORDINATES: protein motif:HMM:NF023459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbK domain-containing protein"
                     /protein_id="HEX6606753.1"
                     /translation="DLTTVGFGMGGYNDPMMQRLADEGNGNAAFVDTLQAAQRLFGEN
                     LTGTLQVIARDAKIQVAFNPAVVSRYRLLGYEKRAIADGDFRNDKVDAGEVGAGQSVT
                     ALYEVVLTSQGTGSVLTVQIRYADALTGQVYEAAQPFARTRFISDAAQAAPDWQLAVA
                     AAGFAGWLRGDEPAVPLAQVQTLAARAAAQLANDADVQEFARLVAQAGSLRK"
     gene            806..1546
                     /locus_tag="VF276_07565"
     CDS             806..1546
                     /locus_tag="VF276_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020879588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX6606754.1"
                     /translation="MIWIGTSGWVYRHWIGCFYPPDLPQRAWLSYYARHFPTVEINRS
                     FYRLPTQDNFRAWAHQAGHHYGFTFAVKASRYLTHHKRLYGPEDPLARLAHAASGLWP
                     HQGPFLYQLPPGMPADAPRLAYFLAHLPPGARHAFEFRDGSWFAPDILHLLGQAGCAL
                     VRAVGGWSTPMDVPDVGPFVYLRFHGGLYDVGFSDGELDFWAGRIAAAHGAGRDVYAY
                     FNNDPTCHAVYDAFRLRDRLAWTGALAP"
     gene            complement(1566..2249)
                     /locus_tag="VF276_07570"
     CDS             complement(1566..2249)
                     /locus_tag="VF276_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606755.1"
                     /translation="MKILIDWTRAETTYTLGALSGYQRAMELQLVSAKEQAESELNRW
                     LESLGEGAFDEGVPVEVYEHQHDYEFAFPRFLRYSFLVLLFFAVENQLNQVCDAIAHR
                     RNLTLRAKDIRASGTIATFRTYLQKVAELSDLGSTLWEPIMELADLRNCIVHALGKVP
                     SEKEGERLRQIVKKTPGLAIGEEDYGQPDVLLITPEYCSQAVSMVDKFFDTLFEAADF
                     GPKHIKISE"
     gene            2463..3014
                     /locus_tag="VF276_07575"
     CDS             2463..3014
                     /locus_tag="VF276_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEX6606756.1"
                     /translation="MAQQKPTPVADLGAVDQETGALNVIIETPKGSRNKFKYDEKRGL
                     FKLSGVLPAGAVFPFDFGYIPATLGGDGDPLDVLLLMDEPAFAGCLVPARLIGVIEAE
                     QTEDGKTERNDRLIAVATDSHDHHQIKSLDQISPNLVEEIEHFFVSYNTIKGKQFTPK
                     GRFGPERAQKLVDAGQAQHKGRQ"
     gene            3156..3704
                     /locus_tag="VF276_07580"
     CDS             3156..3704
                     /locus_tag="VF276_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HEX6606757.1"
                     /translation="MDHLQIGQTRLELRQGDITTIAADVLVNAANDRLAGGTGVNGAI
                     RRAGGPSIAEECKRIGGCPTGSAAITGAGRLQARWVVHAVAPVYRRRPEDAGLLHGAY
                     AASLRLAEEAGARSIAFPSLGTGVFGYPIEEAAPIALDTVAAHVRAGTNLTLVLFVLY
                     SRRDYDLYAHLLADRATARGEQ"
     gene            complement(3744..>4935)
                     /locus_tag="VF276_07585"
     CDS             complement(3744..>4935)
                     /locus_tag="VF276_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606758.1"
                     /translation="GCGACQPTLWHTKDGGRTWIQGTVPGCAWYHSGWLFQFTSAFTG
                     WSVCLPGNPYWVDPQILRKTTDGGLTWRAVYSTEPGRGVGEEYIGDLFFLDNQRGWVV
                     INARRDAGWDTLLRATRNGGRSWQTIATFGKAVDQVRFVSATHGYAVLGETGVLLETL
                     DGGSSWRRIYGPVPTPHLLHLFDPDHGIGVGIPEDVGRLLFTDDGGRHWQSRGSIAFA
                     CGATAPGRVQDLAFADRQRGSLRATCVTRTGATDHDVLLRTTDGGATWTVAPAPTATV
                     SPTPLPPVHIPMGTPAWTLDEYENLLPDPRTGTLAGNPPTNYPEYIIQPNGHSWYLPN
                     PYPQHPCALLKTLDSGQTWTRYNLWTCPQDLAFVDAVHGWMQDMRDHLYTTADGGASW
                     VQVQ"
BASE COUNT          881 a         1638 c         1570 g          846 t
ORIGIN      
        1 gacctgacca cggtagggtt cggcatgggc ggctataacg acccgatgat gcaacggctt
       61 gccgacgagg gcaacggcaa cgcggcgttt gtcgatacgc tccaggccgc gcagcgcctg
      121 ttcggcgaga acctgaccgg cacgctccag gtcatcgccc gcgacgcgaa gatccaggtg
      181 gccttcaacc cggcggtggt cagccgctac cggctgctgg gctacgaaaa gcgggccatc
      241 gccgacggcg acttccgcaa cgacaaggta gacgcgggcg aggtgggcgc ggggcaaagc
      301 gtgacggcgc tctacgaggt ggtgctgacc agccagggca ccggttcggt gctgacggtc
      361 cagatccgct atgccgacgc gctgaccggc caggtgtatg aggctgccca acccttcgcc
      421 cgcacccgct tcatcagcga tgccgcgcag gcggccccgg actggcaact ggcggtggcc
      481 gccgcgggct ttgccgggtg gctgcggggc gatgagccag ccgtccccct ggcccaggtg
      541 caaaccctgg ccgcgcgggc cgccgcgcaa ctggcgaacg atgccgacgt gcaggagttc
      601 gcgcggctcg tggcccaggc gggcagcttg cggaaataga tcgcaccggc gagggccggg
      661 tagcgggaca ggctatccgg ccccttgccg cactccaccg aaacggaatc gccgccgcca
      721 cgtttcaggc agtagccgcc cgccgccctg accgccatta ggcgccgccg gggcgcgggc
      781 atcgccgccg ggcgcgagga tatatatgat ctggatcggc acgtcgggct gggtctaccg
      841 gcactggatc ggctgcttct acccgcccga cctgccgcag cgggcgtggc tcagctacta
      901 tgctcgccac ttccccaccg tcgaaatcaa ccgcagcttc taccggctgc ccacgcagga
      961 caacttccgc gcctgggcgc accaggccgg gcaccactac ggcttcacct tcgccgtgaa
     1021 ggccagccgc tacctgaccc accacaagcg cctctacggg ccggaggacc cgctggcccg
     1081 gctggcccat gccgcgagcg gcctctggcc gcaccagggg ccgttcctct accagcttcc
     1141 gcccggcatg cccgccgacg cgccgcgcct ggcctacttc ctggcccacc tgccgccggg
     1201 cgcgcgccac gccttcgagt tccgcgacgg ctcgtggttc gcgccggata tcctgcacct
     1261 gctggggcag gcgggctgcg cgctggtccg cgccgtcggc ggctggtcca cgcccatgga
     1321 tgtgcctgat gtggggccgt tcgtctatct ccgcttccac ggcggcctct acgacgtggg
     1381 cttcagcgac ggcgaactcg acttctgggc cgggcgcatc gcggcggccc acggcgccgg
     1441 gcgcgatgtc tacgcctact tcaacaacga ccccacctgc cacgccgtct acgacgcctt
     1501 ccgcctgcgc gaccgcctcg cctggaccgg ggcattggcg ccgtgaggta tgggagtggc
     1561 cccggctact cgcttatctt aatgtgcttt ggaccaaaat ccgcagcttc aaatagagta
     1621 tcgaagaact tatcgaccat agatactgcc tgggaacaat actcgggcgt aatgagtagc
     1681 acatctggtt gcccataatc ctcttcacca atagcgaggc caggtgtctt cttcacaatc
     1741 tgccgaaggc gctcgccttc cttttcagaa ggcactttac caagagcgtg aacaatgcag
     1801 ttccgtaagt cggctagttc cataattggt tcccaaagtg ttgagcctag atctgataat
     1861 tccgcaactt tctgaaggta agttctgaaa gtagcaatcg taccactggc tcgaatatcc
     1921 tttgctctca gtgtcaaatt ccgccgatgc gctattgcgt cgcagacttg attcaattgg
     1981 ttttctacag caaagaacag caagacaagg aaggagtaac gtagaaaacg gggaaaggcg
     2041 aactcgtaat cgtgttgatg ctcgtatacc tctacgggca cgccttcatc aaacgccccc
     2101 tctcccaagc tttctaacca tctgttcaat tcggactccg cttgttcttt tgcactgacg
     2161 agttgtaact ccatggctcg ctgataccct gagagtgctc ccagagtgta ggtcgtctcg
     2221 gcacgtgtcc aatcaatcaa gattttcatt attgtggttt cctcctgccg caagtagtgc
     2281 cccctggtta caagaattct ttcgatcgag tcgtgttaat tcttcctcct gcgagtatac
     2341 cacgtggggt tagtggttat cgcaagctgg tatggctggg gacggcatga atcctgatac
     2401 agagaaccgt gcgggcgccg cagccgcagg caccgcagta ctgtggagga gtgagagagc
     2461 gcatggcaca gcagaaaccg acgcccgtgg ccgacctggg cgccgtcgat caggaaaccg
     2521 gggcgttgaa cgtgattatc gagacgccca agggcagccg caataagttc aagtacgacg
     2581 agaagcgcgg gctgttcaag ctaagcggcg tgttgccggc gggggcggta ttccccttcg
     2641 acttcggcta tatcccggcc accctgggcg gggacggcga cccgctggat gtgctcctgc
     2701 tgatggacga gccggctttc gccgggtgcc tggtgcccgc gcggctcatc ggcgtgatcg
     2761 aggccgagca gacggaggac ggcaaaaccg agcgcaacga ccggctcatc gccgtcgcca
     2821 cggactcgca cgatcaccac cagatcaagt cgctggacca gatcagcccg aacctggtgg
     2881 aggagatcga gcatttcttc gtctcctaca acacgatcaa ggggaagcaa ttcacgccca
     2941 agggccgctt cggccccgag cgcgcgcaaa agctggtgga tgcgggccag gcgcagcaca
     3001 aggggcggca ataggggtgc caccaatcca ttcatccgtt caccgcgaag aacgcgaaga
     3061 ttctttatat acgtcgtatt cttcgcgtcc ttcgcgtctt cgcggtgagt ctgtaaacgg
     3121 atgccggggg cgcgggcggg caaggaggta gcgcggtgga ccatctacag atcgggcaaa
     3181 cccggctgga attacgacaa ggcgatatca ccaccatcgc ggcggacgtg ctggtgaacg
     3241 cggccaacga ccggctcgcc gggggcacgg gcgtgaacgg cgccatccgc cgggcgggcg
     3301 gcccgagcat cgccgaggaa tgcaagcgga tcggcggctg ccccaccggc agcgcggcga
     3361 tcaccggggc cgggcggctg caggcgcgct gggtggtcca cgcggtggcc ccggtctacc
     3421 ggcgccggcc cgaggatgcc gggttgctgc acggcgccta cgccgccagc ctgcgcctgg
     3481 ccgaggaagc gggcgcgcgc agcatcgcct tcccctcgct gggcaccggc gtgttcggct
     3541 accctatcga ggaggccgcc ccgattgccc tggacaccgt ggccgcccac gtccgcgcgg
     3601 gcaccaacct caccctggtg ctgttcgtgc tctactcccg ccgcgactac gacctctacg
     3661 cccacctgct ggccgaccgc gccacggccc gcggcgagca gtaggcggcg gtcccggcgc
     3721 cccaggcgcc ggcggatcgg accctattgg acctgtaccc agcttgcgcc gccgtcggcg
     3781 gtcgtgtaaa gatggtcgcg catatcttgc atccacccat ggaccgcatc gacaaacgcg
     3841 aggtcctggg gacaggtcca caaattgtag cgcgtccacg tctgcccgct atcgagcgtc
     3901 ttcagcaagg cacatggatg ctgcggatac gggttgggta agtaccagga gtgcccgtta
     3961 ggctgaatga tgtactccgg gtaattggtg ggcgggttgc cagccagagt gcccgttcta
     4021 ggatccggca gaagattttc atattcgtcc agggtccagg cgggtgtccc catcgggata
     4081 tgaaccggcg gcagcggggt ggggctgacc gtggcggtgg gcgcgggcgc cacggtccag
     4141 gtggcgccgc catcggtcgt ccgcaacagc acgtcgtggt ccgtggcccc ggtccgggtc
     4201 acacacgtgg cgcgtagcga tccccgctgc cggtcggcaa acgccagatc ttgcacacgc
     4261 cccggtgcgg tggcgccgca ggcgaaggcg atggagccgc gggactgcca gtggcgcccg
     4321 ccgtcgtcgg tgaacagcag ccgccccacg tcctccggaa tccccacccc gatcccatgg
     4381 tccgggtcga agaggtgcaa caggtgcggc gtgggcaccg ggccatagat gcgccgccag
     4441 gaactgcccc catcaagcgt ctccagcaag acccccgttt cccccaggac agcatagccg
     4501 tgcgtcgccg acacaaagcg gacctggtct actgccttac caaaggtggc gatcgtttgc
     4561 caggaccgtc cgccattgcg ggtcgcgcgc aagagcgtat cccaacccgc atcgcgacgg
     4621 gcattgataa ccacccagcc ccgctggttg tccaggaaga acaggtcgcc gatatattct
     4681 tcgccgactc cccggcccgg ttccgtcgag tagactgcgc gccaggtcag cccgccgtcg
     4741 gtggttttgc gcaggatctg cggatcgacc caatagggat tgccggggag acagaccgac
     4801 caaccagtga aggcgctcgt gaactggaat agccagccgc tatgatacca ggcacagccc
     4861 ggcacggtgc cctggatcca ggtccggccc ccatccttcg tatgccatag cgtcggctgg
     4921 cacgcaccgc atccg
//
LOCUS       DASUYC010000458         3082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1195252, whole genome shotgun sequence.
ACCESSION   DASUYC010000458 DASUYC010000000
VERSION     DASUYC010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195252"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            568..2178
                     /locus_tag="VF276_07590"
     CDS             568..2178
                     /locus_tag="VF276_07590"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HEX6606759.1"
                     /translation="MQTLDPRPIDNRELDQTRTLIPPPDVEPTAAPILVDPAHSVARN
                     TLIMLVSQMITFAMTFIWTVAVQSGLGSATFGKLFWAQSLALTGGVFMDAGIATYLTK
                     QVARDRAHSTRYLSTAVLLRTVSTVLVYLAILGWAVATGEHNDGESLLAVVLVGLAIV
                     VASFTQACAATFQGNENMLWQALGTIAEKVTVTALSVTLLVLGYGLPAVAAVMLIGAA
                     MNLLLIGSRAIRLGWVRPQFDRRLAWQMIRGGAPFFLWAAFGVIYQRNAAIQLKALTD
                     AHTDGLFGAAVRMYETLSFVPYIFQAAVLPVLSRTFVNAADAMVRTSRRSLDLIVLAA
                     LPIGLGVVLLAPEIIALVGGLPEYDGAVLPLRILGFSLVPLYADMILATILVSVDKQK
                     QWAFVSVGAALVNPLLNWWLIRQTQAMYGNGAIGSAFVTFLTELVIFFFYLALVPRGV
                     LGASNLRFAARAVVATLLMGAAIWLILPALQSVVPGGPLARGGAAVVLAGAGVLGAGV
                     FGGAALLLRLVGRDELALVRKALRRGAA"
     gene            2196..2750
                     /locus_tag="VF276_07595"
     CDS             2196..2750
                     /locus_tag="VF276_07595"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX6606760.1"
                     /translation="MKRALLAIISYITNHIVSAIPFYTVRHAWYRRVLGIRLGRGAAL
                     CMDQFIWFYSLSQVRRDGVRIGPDSLINRGCVLDARGPLRIGAHVSISPHVAILTTQH
                     LMDDPRFVTETKPVVIEDYAWIGMRAMIMPGVTIGEGAVVAAGAVVSRDVEPYTVVGG
                     VPAKPIGRRKRPARYELAFHPLFE"
     gene            2758..>3082
                     /locus_tag="VF276_07600"
     CDS             2758..>3082
                     /locus_tag="VF276_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606761.1"
                     /translation="MRILWLCSYAPYPPDFGGARRTYNLLAQAAHAGHSIDLLALATG
                     DPAQDAATVDALRALCAHVELVRDPWALPAALGAADPAAVARKRRGQLQSLLSRRSYQ
                     FYSHYS"
BASE COUNT          429 a         1042 c         1063 g          548 t
ORIGIN      
        1 ttgggcggcc agacggtgat ccaggccagg ccgtcgccgt agaagctgac ctgcccaccc
       61 tggcgggcgc tccgcttcat cttcagccct tcctcgccga agaagcgctc ggcctcgtcc
      121 agcacctgct ccggcttcag gtgggtgtgt gtttcatagc gcatggagta tgtcacctct
      181 ttcacattgc cgatggatcg gcatctcaca gcgcgagtct agcataggcc gggccggatc
      241 gtcaacgcgg ctccccctgc ctgcgcgagc aggagcgact gtatgatgag cccgcgcgcc
      301 cggtagcgag atcgcccggc ccgtcaacgg gcgagctacg tcaaccaagc ccgcctgcgc
      361 gggctcgacc agtggaggga accaacaggc cgcgcaggcg gccttcgtaa agctagcagg
      421 cggcttgtgg cgtatacctc agcccggcag actcagctat ttctgtcgag ttgttaattt
      481 ccgcgcgtta cggcaccgtt cctgctattg caggccacgg gccgccgcag taccgaatcc
      541 caggccgccc tagccggaga ctgacgcttg caaactctcg atcctcgccc gatcgacaac
      601 cgcgaactgg accagacgcg cacgctgatc ccgccgccgg acgtggaacc caccgccgcg
      661 ccgatcctgg tcgatccggc gcacagcgtg gcgcgcaaca ccctgatcat gctggtgtcc
      721 cagatgatca ccttcgccat gacctttatc tggacggtgg cggtgcaatc ggggctgggc
      781 agcgcgacgt tcggcaagct gttctgggcg caatcgctgg cgctgaccgg gggcgtgttc
      841 atggacgcgg gcatcgccac ctacctgacc aagcaggtgg cgcgcgaccg cgcccactcc
      901 acgcgctacc tcagcacggc ggtgctgttg cgcacggtca gcaccgtgct ggtctacctg
      961 gcgatcctgg gctgggccgt ggcgaccggc gagcacaacg acggcgagag tctgctggcc
     1021 gtcgtcctgg tggggctggc gattgtggtg gcgagcttca cgcaagcctg cgccgccacg
     1081 ttccagggca acgaaaacat gctctggcag gccctgggca ccatcgccga aaaggtcacg
     1141 gtgacggcgc tgagcgtgac gctgctggtg ctgggctacg ggttgcccgc ggtggccgcc
     1201 gttatgctca tcggcgcggc gatgaacctg ctgctgatcg gcagccgggc gatccggctc
     1261 ggctgggtgc ggccccagtt cgaccggcgg ctggcctggc agatgatccg cggcggggca
     1321 ccgtttttcc tctgggcggc cttcggcgtg atctaccagc gcaacgccgc gatccagctc
     1381 aaggctctca ccgacgcgca caccgacggc ctattcggcg cggcggtgcg catgtacgag
     1441 acgctgtcgt tcgtgcccta tatcttccag gcggcggtgc tgccggtgct gtcgcgcacc
     1501 ttcgtcaacg ccgcggacgc catggtgcgc acctcgcggc gcagcctcga cctgatcgtg
     1561 ctggccgcgc tgcccatcgg cctgggcgtg gtgttgctgg cgccagagat catcgccctg
     1621 gtcggcgggc tgcccgaata cgacggcgcg gtgctgccgc tgcgcatcct cggcttcagc
     1681 ctggtgccgc tctacgccga catgatcctg gcgacgatcc tggtctcggt cgacaagcag
     1741 aagcaatggg cctttgtctc ggtgggcgcg gcgctggtca atccgctgct gaactggtgg
     1801 ctgatccgcc agacgcaggc catgtacggc aacggggcca tcggctcggc cttcgtcacg
     1861 ttcctgaccg agttggtcat tttcttcttc tacctggcgc tggtgccgcg cggggtgctg
     1921 ggggcgagca acctgcgctt cgcggcgcgg gcggtggtcg ccacgctgct catgggcgcg
     1981 gcgatctggc tgatcttgcc cgcgctgcaa agcgtggtgc ccggcgggcc gctggcacgg
     2041 ggcggggcgg cggtggtgct ggccggcgcg ggggttctcg gagcgggcgt gttcggcggc
     2101 gccgccctgc tgctgcggct ggtcggtcgc gacgagctgg ccctggtgcg caaggcgctg
     2161 cgccggggcg cggcgtaggc gggagagtag aaacgttgaa gcgtgcgtta ctggcgatca
     2221 tttcgtacat cactaatcac atcgtcagcg cgatcccgtt ctatacggtg cggcacgcct
     2281 ggtaccggcg ggtgctcggt atccggctgg ggcggggcgc agccctctgc atggaccagt
     2341 ttatctggtt ctacagcctg tcgcaggtgc gccgcgacgg cgtgcgtatc ggccccgaca
     2401 gcctgatcaa ccggggctgc gtgctcgacg cgcgcggccc gttgcgcatc ggcgcgcacg
     2461 tgagcatctc gccgcacgtc gccatcctga ccacgcagca cctcatggac gacccccgct
     2521 tcgtcaccga gacgaagccg gtggtgatcg aggactacgc ctggatcggc atgcgggcga
     2581 tgatcatgcc cggcgtgacc atcggcgaag gcgcggtggt cgctgcgggg gccgtggtca
     2641 gccgcgatgt tgagccctat accgtagtgg gcggggtgcc cgcgaaaccg attggccggc
     2701 gcaagcgccc ggcccgctac gaattggcct ttcacccgct gtttgagtga ccctgcgatg
     2761 cgcattctct ggctctgttc ctacgcgccc tatcccccgg acttcggggg cgcccggcgc
     2821 acctataatc tgctggcaca ggcggcgcac gccgggcaca gcatcgacct gctggccctg
     2881 gcgacgggcg acccggcgca ggacgcggcc accgtggatg cgttgcgtgc cctgtgcgcc
     2941 cacgtcgaac tggtgcgcga cccatgggcc ttgcctgcgg cgctcggcgc ggcggacccg
     3001 gcggcggtgg cgcgcaagcg gcgcggacaa ctgcaatcgc tgctgtcgcg ccgttcctac
     3061 cagttctact cgcattactc cg
//
LOCUS       DASUYC010000459         2509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_9386,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000459 DASUYC010000000
VERSION     DASUYC010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9386"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="VF276_07605"
     CDS             complement(<1..946)
                     /locus_tag="VF276_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX6606762.1"
                     /translation="MLSFIARRLGLLIPTFFGVTLLTFALIRLIPGDPVEVMMGERRV
                     DPEMHAEAMHRLGLDKPLPAQYLDYITKLAQGDLGESLRTREGVWDEFLTLFPATLEL
                     SLAALLFAGTLGVIAGVIAALKRGSLFDHGVMGISLAGYSMPIFWWGLLLIMFFSVYL
                     GWTPVSGRIDLLYDIEPVTGFMLIDTLLSEEEGAFKDALMHLILPAIVLGTIPLAVIA
                     RMTRSAMLEVLREDYIRTARAKGLSPARVVFVHGLRNALIPVLTVFGLQVGTLLAGAV
                     LTETIFSWPGIGKWLIEAIGARDYPVVQNGILLIACLVI"
     gene            complement(1001..>2509)
                     /locus_tag="VF276_07610"
     CDS             complement(1001..>2509)
                     /locus_tag="VF276_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016490932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6606763.1"
                     /translation="EAAPEGFDIVQYTAATTADASAETVYERLVQFAPGSTRIIPALA
                     ESWEISADGLTYTFTLRQGVKFHETPWFKPSRELNADDVLWSFQRQRDKEHDWHELAP
                     RGFPYFEAMGFAELIDEIEALDDHRVRFTLKHPEAPFLADLAMGFTSIYSAEYAEQLL
                     DAETPGKLNSHPIGTGPFVFERYSKDAMVRFAANPYYWAGKPQIERMVLAITPDPNVR
                     IQQLKAGACQVAVFPRPSDVPGLRQNKDLQVLELDSLLTAYVAINTRHKPLDDVRVRQ
                     AINLAFDRQAYLRAQYGEGNATLAAAPYPATLLGHDERLQPWPKDLERARQLLAEAGH
                     EKGLKLSIWTRPGGGPTNPNPGIGAQMLQADLAAIGIAADIRVFEWGELIKRAKNGEH
                     DLVFMGWAGDNGDPDNFLTPNLSCAAAASGENQAGWCHQEFDQLIREARRVAEPERRA
                     ALYREALAIFHQQAPWIALAHPKQFAVLRKDVEGFVLSPLGSNNFTHVKRKP"
BASE COUNT          406 a          776 c          917 g          410 t
ORIGIN      
        1 ggatcaccag gcaggcgatc agcaggatgc cgttctgcac caccgggtag tcgcgggcgc
       61 cgatggcctc gatcagccac ttgccgatgc ccggccagga gaagatggtc tcggtcagca
      121 cggcgcctgc cagcagggtg ccgacctgca ggccgaacac ggtgagcacc gggatcagcg
      181 cgttgcgcag gccatgcacg aacaccacac gggccgggga caggcccttg gcacgggcgg
      241 tacggatata gtcctcgcgc agcacttcga gcatcgccga gcgggtcatg cgcgcgatca
      301 ccgccagggg aatggtgccc agcacgatgg ccggcaggat caggtgcatc agggcgtcct
      361 tgaacgcgcc ttcctcttcg ctgagcaggg tgtcgatcag catgaaaccg gtgaccggct
      421 cgatgtcgta gagcaggtcg atgcgcccgg agaccggcgt ccagcccagg tacaccgaga
      481 agaacatgat cagcagcagg ccccaccaga agatcggcat cgagtagccg gccagcgata
      541 tgcccatcac gccgtggtcg aacagcgagc cgcgcttgag ggcggcgatc actccggcga
      601 tcacgcccag ggtgccggcg aacagcaggg ccgccaggga cagctccaga gtcgccggga
      661 acagggtgag gaactcgtcc cacacgccct cgcgggtgcg cagggattcg cccaggtcgc
      721 cctgggccag cttggtgatg tagtccaggt actgggccgg cagcggcttg tccaggccca
      781 ggcggtgcat ggcctcggcg tgcatctcgg ggtcgacacg gcgctcgccc atcatcactt
      841 ccacggggtc gccggggatc agacggatca gcgcgaaggt cagcaaggtg acgccgaaaa
      901 aggtggggat cagcagcccc aggcgtctgg cgataaagga cagcattgcg ttgtggtact
      961 ccgcatcagg ttgagcccgc gccttcgtgg ggcgcggctg ttatggcttg cgctttacat
     1021 gggtgaaatt gttggagccc agcgggctga gcacgaagcc ctcgacatcc ttgcgcagca
     1081 cggcgaactg cttgggatgg gcgagggcga tccagggtgc ctgctggtgg aagatcgcca
     1141 gcgcctcgcg gtacagcgca gcgcggcgct ccggctcggc gacccggcgg gcctcgcgga
     1201 tcagctggtc gaactcctga tgacaccagc cggcctgatt ctcgccactc gcggcggcgg
     1261 cgcaggacag gttgggggtc agaaaattgt ccgggtcgcc gttgtcgccg gcccagccca
     1321 tgaacaccag atcgtgctcg ccgttcttgg cgcgcttgat cagctcgccc cactcgaaca
     1381 cgcggatatc ggcggcgatg ccgatggcgg ccaggtccgc ctgcagcatc tgcgcgccga
     1441 tgcccgggtt ggggttggtc gggccgccgc cggggcgggt ccagatcgac agtttcaggc
     1501 ctttttcatg accggcctcg gccagcagct ggcgggcgcg ctcgaggtcc ttgggccagg
     1561 gctgcaggcg ctcgtcatgg ccgagcaggg tggccgggta gggcgctgcg gccagggtgg
     1621 cgttgccttc gccgtactgg gcgcgcaggt aggcctggcg gtcgaaggcc aggttgatgg
     1681 cctggcgcac gcgcacatcg tccagcggct tgtgtcgggt gttgatggcc acgtaggcgg
     1741 tgagcagcga gtccagctcc agcacctgca ggtccttgtt ctggcgcagg ccgggcacgt
     1801 cgctcgggcg cgggaacacg gccacctggc aggcgccggc cttgagctgc tggatgcgca
     1861 cgttggggtc gggggtgatg gccaacacca tgcgctctat ctgcggcttg ccggcccagt
     1921 aatacggatt ggccgcgaag cgcaccatgg cgtctttgga atagcgctcg aagacgaagg
     1981 gcccggtgcc gatcgggtgg ctgttgagct tgcccggcgt ctcggcgtcg agcagctgtt
     2041 cggcgtactc ggcggaatag atggaggtga agcccatcgc caggtcggcc aggaagggcg
     2101 cttcggggtg tttcagggtg aagcgcacgc ggtggtcgtc gagcgcctcg atctcgtcga
     2161 tcagctcggc gaagcccatg gcctcgaaat aggggaagcc gcgcggggcc agctcgtgcc
     2221 agtcatgctc cttgtcgcgc tggcgctgga agctccatag cacgtcgtcg gcattcagct
     2281 cgcggctcgg cttgaaccag ggcgtctcat gaaatttcac accctggcgc agggtgaagg
     2341 tgtaggtcag gccgtcggcg ctgatctccc agctctcggc cagggcggga atgatccggg
     2401 tgctgccggg ggcgaactgc accaggcgct cgtagaccgt ctcggccgag gcgtcggcgg
     2461 tggtggcggc ggtgtactgg acgatgtcga agccttccgg ggcggcctc
//
LOCUS       DASUYC010000460         6266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_604641,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000460 DASUYC010000000
VERSION     DASUYC010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6266
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604641"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="VF276_07615"
     CDS             <1..1137
                     /locus_tag="VF276_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020580291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEX6606764.1"
                     /translation="EFKTPWGTMPGPFSLAPEDPGSLALVRSLYDELLPYFSSTQFNV
                     GCDETFDLGQGRSKAICEERGTERVYLDYLLQIYQEVKARDHQMQFWGDIIVEKPELI
                     GELPKDSVALEWGYEADHSFAEHCPQFAASGIPFYVCPGTSSWCSLAGRTDNAVGNLL
                     SAAENGRAHGALGYLNTDWGDLGHWQVLPISYLGFAVGAAYSWALDANRDMDIAAVVS
                     THAFGDPTGNMGRVAYDLGNVYQAPGVLIHNASLLFWTLQLPLGRTRVPEGQQADYAG
                     TRAAIDAAMAPLSAARMQRPDAALIVEEFENTARLMRHACRRGQWLVDCEHGDTAATR
                     HALDADLRDIIREYERIWLRRNRPGGLADSVARLQKARADYGVA"
     gene            1367..1954
                     /locus_tag="VF276_07620"
     CDS             1367..1954
                     /locus_tag="VF276_07620"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX6606765.1"
                     /translation="MTDISYRLQTIRRRALIGTLGAALLVSLAACGTAGDPAGSAPPP
                     ATVAPTATVAVAAEAAATATPSMDAGMDMSTMPPDAPTSAEATPTVAAPASDASAGNA
                     VQVQATLREWALDLNQTEVPAGTVRFVVNNQGRFAHNFTIKDANNTVLGKTPTFAADK
                     GPQVVEVTLAPGTYTVYCSLPGHAAQGQQNTIVVK"
     gene            complement(2043..2684)
                     /locus_tag="VF276_07625"
     CDS             complement(2043..2684)
                     /locus_tag="VF276_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012255949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEX6606766.1"
                     /translation="MSEPAVPRLVPAAPATAEIRVSNSRFIANAAPAATVEEARAFLA
                     AIRAAMPDATHHVYAYRIGHGATTTEGQSDAGEPSGTAGRPVLAVVRGSGLGDLIVVV
                     TRYFGGTLLGTGGLVRAYGDAAKAVLAVLPQTEKIDYTRLRITLPYAAYELSRRQIAA
                     HAGTLTGETFAVEVTLEVRLPSAQAGLLADALRETTAGQARVEILPSPPAAGD"
     gene            3475..>6266
                     /locus_tag="VF276_07630"
     CDS             3475..>6266
                     /locus_tag="VF276_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606767.1"
                     /translation="MRSRRTPLQILGIALAAVLALTTALTAQFTAAAGPETREVSLST
                     ALTSGTAYDRNPSLLHAADGTYYLFFARSTVAPCVRPSCNPDNNTYNIFYMTSADGLT
                     WGAPTQLSDRTGIGGTFYGRTVAATQDAAGTIWVFWASGGNSADLYYYTNAGSGWSAR
                     AQLSDAAYFNAEAVATPDGKVWLFYEDGSTTGIYARYWNGTTWSAPTLVAAGMSLPKA
                     IVDGSTFRLVMVDGATGTDYITSSADGITWTAPVVVAAPGGGVTNWDPTIFKDGAGKY
                     NIFLAPDLGTGPQRIAWTQSADGTTWPGSNTTVTAARYGANDWWDYWPEAGQIGSATY
                     LFYTSEKAGTTRGSGHILMTRIYWDPASNHYEAIQPAIDAAVPGDTANLAAGTYAESI
                     VINKDSLTLQGNTAATTTLQGAGTGTGIAFSGARSNISIKRLTATNWQYGLSLPTGPL
                     NTVLIEDVAAVNNTLHGIWVQASPINGVTLRRVNASGNSGGTGRGVWLINGAKSNITI
                     EDGTYNNNGLVGIDISDGSVTGLAITGNTVTGNGDSGIGVLGAQGTGANLVANNTVTN
                     NGRFGIEIKNSAGNGALSGPGSVVVENNTVSRTVAATRVEDYAGILVIRRSPQAGVNP
                     DQPSGIVIRNNQVSGYHRKPTGSTGDGFGIVVEGLNEQVLDNTVTGNDVGIQVQGGNP
                     SINQQGTAYFDRGNAAAGNGTVTGNRITGNDIGGRNVGGPAASTFNGENNWWGSANGP
                     TAPGNTYNVGAQGDSAVNDIDYVPWLDAAPPAGQPFAPVTTTDPPGSYSSIQAGVTGS
                     NIRGTLNAEAGTFTENVIISRTLTVAGAGRGTIVIPAVSNPNCNSGGGGSLCGGAASN
                     VFLVRANGVTIHDLIVNGDNPTLTSGIVRGGADLDARNGIITDNVAGNFATLTVYRVT
                     VYSTYLRGIQGSGGSG"
BASE COUNT         1087 a         2266 c         2003 g          910 t
ORIGIN      
        1 gagttcaaga cgccctgggg cacgatgccc ggcccgttca gcctggcgcc cgaagatccc
       61 ggcagcctgg ccctggtgcg cagcctctac gacgagttgc tgccctattt ctccagcacc
      121 cagttcaacg tgggctgcga cgaaactttc gacctgggcc agggccgcag caaggctatc
      181 tgcgaggagc ggggcaccga gcgcgtctac ctggactatc tgctgcaaat ctaccaggag
      241 gtgaaagcgc gcgaccacca gatgcagttc tggggtgaca tcatcgtgga aaagcccgaa
      301 ttgatcggcg aactgccgaa ggacagcgtc gcgctggagt ggggctatga ggccgatcac
      361 tcgttcgccg agcattgccc gcagttcgcg gcgtcgggca tccccttcta cgtctgcccc
      421 ggcacatcgt cctggtgttc gctggccggg cgcacggaca atgccgtggg caacttgctg
      481 agcgccgccg aaaacgggcg cgcgcacggc gccctaggct atctgaacac cgactggggc
      541 gacctgggcc actggcaggt gctgccgatc agctacctgg ggtttgccgt cggcgccgcc
      601 tattcctggg cgctcgacgc gaaccgggat atggacatcg ccgccgtggt cagcacccat
      661 gccttcgggg acccgaccgg caacatgggg cgcgtggcct acgacctggg caacgtctac
      721 caggcgccgg gcgtgctcat ccataacgcg tcgctgcttt tctggacgct gcaactgccg
      781 ctgggccgca cgcgcgtgcc cgagggccag caggccgact acgcgggcac gcgggccgcg
      841 atcgacgcgg ccatggcccc gctgagcgcg gcccgtatgc aacgccccga cgcggcgctg
      901 atcgtggagg agttcgagaa cacggcccgc ctgatgcgcc atgcctgccg ccgcgggcag
      961 tggctggtcg actgcgaaca cggcgacacc gccgccaccc gccacgcgct cgacgccgat
     1021 ctgcgcgaca tcatccgcga gtacgagcgc atctggctgc ggcgcaaccg gcccggcggc
     1081 ctcgccgaca gcgtggcgcg cctgcaaaaa gcgcgggccg attacggcgt cgcgtaaccg
     1141 tccgcctcta tccgcccaag tctagccact acctataacc cgccgggcca ctatcacagc
     1201 cgggcgggtt tgcttatgct tgcggcctgg gtgataccgt gagacggccc ggcgcgcccg
     1261 gattcttacg caattatcag tgtattctga agattctctg tagattggcc gctataatgg
     1321 cctcttgcta ccgactatta gcagactcgg aggaatctat tttcgcatga cagacatttc
     1381 ctatcgctta caaactatcc ggcgccgcgc gctgatcggc acgctggggg cagcgctcct
     1441 tgtgagtctg gccgcctgcg gcaccgccgg cgaccccgcc ggcagtgccc cgccgcccgc
     1501 aaccgtcgct cccaccgcca cagtggccgt cgccgccgag gccgccgcca cggcgacccc
     1561 cagcatggac gccggcatgg acatgagcac catgccgccc gacgcgccga cgagcgcgga
     1621 ggcgacgccg acagtcgccg ccccggcgag cgacgcgagc gccgggaacg cggtccaggt
     1681 gcaggcgacg ctgcgcgaat gggcgcttga tctcaaccag accgaggtgc ccgccgggac
     1741 ggtgcgcttc gtggtcaaca accagggccg gttcgcgcac aacttcacga tcaaggacgc
     1801 gaacaacacg gtgctcggca agacgcccac cttcgcggcg gacaaaggcc cccaggtggt
     1861 cgaagtgacc ctggcgccgg gcacctacac ggtgtattgc agcctgcccg gccacgccgc
     1921 ccagggccag cagaatacca tcgtcgtcaa ataacgcggg ctccggccct catatagcgg
     1981 aatgtgtagc acgcggaggc gcgtcccacc tggggcgcgc ctcttggcgt ggtggacagg
     2041 agtcagtcgc cggcagcggg cggtgagggc aggatttcga cgcgcgcctg cccggccgtc
     2101 gtctcgcgca acgcatcggc caagaggccg gcctgggcgc tgggcagccg gacctccaac
     2161 gtgacctcga cggcgaaggt ttcgccggtc agggtgccgg cgtgcgcggc gatctggcgg
     2221 cggctcagtt catacgccgc gtagggcaga gtgatccgca gccgggtata gtcgatcttt
     2281 tcggtctgcg gcagcacggc cagcaccgcc ttggccgcgt cgccataggc gcgcaccagg
     2341 ccgccggtgc cgagcaatgt gccgccgaag tagcgggtga cgaccacgat cagatcgccc
     2401 aggccgctgc cgcggaccac ggccagtacc gggcggcccg ccgtgcccga cggctcgccc
     2461 gcgtcgctct ggccttcggt ggtggtcgcg ccgtggccga tgcggtaggc gtagacgtga
     2521 tgggtggcat cgggcatggc cgcgcggatg gcggcgagga aggcccgtgc ctcctccacc
     2581 gtggcggccg gcgcggcgtt ggcgataaag cgggaattgc tgacccggat ctcggcggtg
     2641 gcgggcgccg ccgggaccaa ccggggcacg gcgggttcgg acatggcatc tccggcgggc
     2701 ggcaggcccg cgctccccct agtatagcat agcccccgcg ccggacgccg cgcaccgaac
     2761 tgcggtggtg gcgccccagc gggctactgc tacagcttgt ttcccttcat tcagcaatca
     2821 aatctgctat taagcataat tttctcgttt agtggcggac tcgcgcaata gatgttataa
     2881 tactcgccgg cgcaaagatt acgaggcgaa attcaagcca agcgtcatta aaccctcgcc
     2941 tcggatacct gccgccgggc ccgtacactc catccaccgc gggcaccccc ggctgctcta
     3001 cctggtagca ggcccatccc tggccgtcgc gggcgccacg gtccaggcta tcgttacacc
     3061 gtggccctgt ggtacacctc attcggcctc cgtccggcca acacacgcgc gtggtacgcc
     3121 gtccccggcg ttttgcggtg cgtgcctggt tgtgcgcttc ccgcttttcg agtccaacag
     3181 gtgcacaggt cctggccgga tcgcccggca cgatatcgcc gcgatcccgg tcacaagctc
     3241 gaccctatcc gctcccgata cccatccatc ggcgcagcgg cagacgtgcc gtgccggaag
     3301 gcatccggcg gtgcccgcgg agacgcggcg ggtacccggc tggcgcccac cccgcgcacg
     3361 gctggtaagg aggttggccc cactttatcc ccccaggttt gtccggcccg gcataggccc
     3421 gcccgccggc aatgacgccc ttattcgctc gctttattca ggagagatat agcaatgcgt
     3481 tcccgcagaa ctcccttgca gatcctcggc atcgcgctgg cggccgtcct ggccctgacc
     3541 accgcgctga cggcgcaatt caccgccgcc gccgggcccg aaacccgcga ggtcagctta
     3601 tcgaccgcgc tgaccagcgg caccgcctat gaccgcaacc cgtcgctgct gcacgccgcc
     3661 gacggcacct actacctgtt cttcgcccgc agcacggtcg ctccctgcgt ccgcccgagc
     3721 tgcaatcccg acaacaacac ctacaacatc ttctacatga cctccgcgga cggcctgact
     3781 tggggcgcac ccacccagct ctccgaccgc accggcattg gcggcacctt ctatggtcgc
     3841 accgtcgccg ccacccagga cgccgccggc accatctggg tcttctgggc cagcggcggc
     3901 aacagcgccg acctctacta ctacaccaat gccggcagcg gctggtcggc ccgcgcccaa
     3961 ttgagcgacg cggcctactt caacgccgaa gccgtcgcca ctcccgacgg caaggtctgg
     4021 ctgttctacg aggacggcag caccaccggc atctatgccc gctactggaa cggcaccacc
     4081 tggtcggccc cgacgctggt ggccgccggc atgtcgctgc ccaaggccat cgtcgacggc
     4141 agcacgttcc ggctggtgat ggtcgacggc gccaccggca ccgactacat cacctcgtcg
     4201 gcggacggca ttacctggac ggccccggtc gtcgtggcgg cgccgggcgg cggcgtcacc
     4261 aactgggacc cgacgatctt caaggatggg gcgggcaagt acaacatctt cctggcgccg
     4321 gacctcggca cggggccgca gcgcatcgcc tggacacagt cggcggacgg cacgacgtgg
     4381 ccgggcagca acaccacggt gacggcggcg cgctacgggg ccaacgactg gtgggattac
     4441 tggcccgagg ccggccagat cggcagtgcc acctacctgt tctacacctc ggagaaggcg
     4501 ggcaccacgc ggggcagcgg ccacatcctg atgaccagga tctactggga cccggccagc
     4561 aaccactatg aggccatcca gcccgccatc gacgcggcgg tcccaggtga caccgccaac
     4621 ctcgccgccg gcacctacgc cgagagcatc gtgatcaaca aggacagcct gacgctgcag
     4681 ggcaacaccg ccgccaccac gaccctgcaa ggggcgggca cgggcacggg tatcgccttc
     4741 agcggggcgc gcagcaacat cagcatcaag cgcctgaccg ccaccaactg gcagtacggc
     4801 ctcagcctgc ccaccggccc gctgaacacc gtgctgatcg aagacgtggc cgcggtgaat
     4861 aatacgctcc acggcatctg ggtccaggcc tcccccatca acggagtcac gctgcggcgg
     4921 gtcaacgcct ccggcaacag cgggggcacg ggccgcggcg tctggctcat caacggcgcc
     4981 aagagcaaca tcaccatcga agacggcacc tacaacaaca acggcctggt cggcatcgac
     5041 atcagcgacg gcagcgtgac cgggctggcc atcaccggaa acaccgtcac cggcaacggc
     5101 gactcgggca tcggcgtgct gggcgcgcag gggacgggcg ccaatctggt cgccaacaat
     5161 accgtgacca acaacgggcg cttcggcatc gagatcaaga attcggccgg caacggcgcg
     5221 ctgagcggtc ccggcagcgt ggtggtggag aataacaccg tctcgcgcac ggtggccgct
     5281 acccgcgtcg aagactacgc cggcatcctc gtgatccgcc ggtccccgca ggcgggcgtc
     5341 aaccccgacc agcccagcgg catcgtgatc cgcaacaacc aggtcagcgg ctaccaccgc
     5401 aagcccaccg gctcgaccgg cgacggcttc ggcatcgtgg tcgagggcct caacgagcag
     5461 gtgctggata acaccgtcac cggcaacgac gtgggtatcc aggtccaggg cggcaacccg
     5521 tcgatcaacc agcagggcac ggcctacttc gaccggggca atgccgcggc gggcaacggc
     5581 acggtgacgg gcaatcgcat caccggcaac gacatcggcg ggcgcaacgt gggcggcccg
     5641 gcggccagca ccttcaacgg cgagaacaac tggtggggca gtgccaacgg gcccaccgcc
     5701 cccggcaaca cctacaacgt gggcgcgcag ggcgactcgg cggtcaacga catcgactac
     5761 gtgccctggc tggacgccgc accgcccgcc gggcagccgt tcgccccggt cacaacgacc
     5821 gacccgcccg gcagctactc gtccatccag gcgggcgtga ccggctccaa catcaggggc
     5881 actctcaacg ccgaggccgg caccttcacc gagaacgtga tcatctccag gacgctgacc
     5941 gtggccgggg ccgggcgggg taccatcgtc atcccggcgg tgtccaaccc caactgcaac
     6001 agcggcgggg gcggctcgtt gtgcggcggc gccgccagca acgtcttcct cgtgcgcgcc
     6061 aacggcgtca cgatccatga cctcatcgtg aacggcgata atcccaccct caccagcggc
     6121 atcgtccgcg gcggcgccga tctcgatgcc cgcaacggta tcatcaccga caatgtagcc
     6181 ggcaacttcg ccaccttgac cgtctaccgt gtcaccgtct atagcaccta cctgcgcggc
     6241 atccagggat cgggcggcag cggcta
//
LOCUS       DASUYC010000461         2004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1212574, whole genome shotgun sequence.
ACCESSION   DASUYC010000461 DASUYC010000000
VERSION     DASUYC010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1212574"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VF276_07635"
     CDS             <1..497
                     /locus_tag="VF276_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013557560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX6606768.1"
                     /translation="AVALTRSGTIGVLATPATLRGALLEEVIARFATPAGVRVVKDPG
                     CGLVEAVEAGALDTPATRARLRQALAPLLGAGADALVLGCTHYPFLRAGIAALAGPHV
                     ALVDSGLGVARQTHRLLHERGWANPGPAPGRLDLYTSGDPAQVGPVMCRLLGEELPVC
                     ASWR"
     gene            479..1633
                     /locus_tag="VF276_07640"
     CDS             479..1633
                     /locus_tag="VF276_07640"
                     /inference="COORDINATES: protein motif:HMM:NF020318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1786 family protein"
                     /protein_id="HEX6606769.1"
                     /translation="MRILAVDIGTGTQDILLFDSDEPIENSMQLILPAPTMAVAGRIR
                     AATAAGRPVVLDGTIMGGGPCSWAAEDHLRAGYALYATPDAARTFDDDLERVAAMGVT
                     LIDPAEVAGREAAGALRVTMRDFDPAAILAALAGFGVAPRVDALCVAVFDHGNAPPGT
                     SDRRFRFDYLAERLAAGGRTDLAAFAFLRDAIPPAMTRLHAAAHSPTGVLAELPLLAM
                     DTGPAAVLGTLEVPALRAAAMAPAGALVVNVGNFHTLAFHLAGGAVAGLFEHHTGELT
                     AAQLAGLLDRLAAGTLTNDEVFHTQGHGALVLAPTAAPPALCAVTGPRRAKLAAASLS
                     WPLLPAVPHGAMMLTGCFALLRALAHHWPPAQAPVAAVLGADEWPVVSGQ"
     gene            complement(1757..>2004)
                     /locus_tag="VF276_07645"
     CDS             complement(1757..>2004)
                     /locus_tag="VF276_07645"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEX6606770.1"
                     /translation="TRGQLAKIIALAKGWTLLNPPTAHFRDVAPGSVFYQHIETVVAK
                     GVVSGYTCGTGCLEYRPGNNGTRGQLSKMLYFAVTQR"
BASE COUNT          243 a          775 c          703 g          283 t
ORIGIN      
        1 cggcggtcgc cctcacccgc tcgggcacca tcggcgtgct ggcaaccccg gccacgttgc
       61 gcggcgcctt gctggaggaa gtcatcgcgc gcttcgccac gcccgcgggc gtgcgggtag
      121 tcaaggaccc tggttgcggc ctggtggaag ccgtcgaagc cggggcgctc gatacacccg
      181 ccacccgcgc ccggctgcgg caagccctgg cgccgctgct gggggccggc gccgacgcgc
      241 tggtgctcgg ctgcactcac tacccgtttc tgcgcgccgg gatcgccgcc ctggccgggc
      301 cgcacgtggc cctggtcgat tccggcctgg gcgtggcccg gcaaacccac cggctgttgc
      361 acgagcgggg ctgggccaat cccggccccg cgcccggccg gctcgacctc tacaccagcg
      421 gcgacccggc ccaggtaggc ccggtcatgt gccgcttact cggcgaggag ctgcccgtat
      481 gcgcatcctg gcggtagaca tcggcaccgg cacgcaagac atcctgctgt tcgacagcga
      541 cgagccgatt gagaattcga tgcagttgat cctgcccgcg cctacaatgg ctgtggccgg
      601 gcgcatccgc gccgcgacgg cggcgggccg gcccgtcgtg ctcgacggca cgatcatggg
      661 cggcgggcca tgctcctggg ccgccgagga ccatctgcgg gcgggctacg cgctctatgc
      721 cacgcccgac gcagcgcgga ccttcgacga cgacctggag cgggtcgcgg ccatgggcgt
      781 caccctgatc gatcccgccg aggtggccgg gcgcgaggcc gcgggcgcgc tgcgcgtcac
      841 catgcgcgac ttcgaccccg cggcgatcct ggccgcgctg gccggcttcg gcgtggcccc
      901 gcgcgtggac gcgctctgcg tggccgtctt cgaccacggc aacgcgccgc ccggcaccag
      961 cgaccggcgc ttccgcttcg actacctggc cgagcggttg gcggcgggcg ggcgcaccga
     1021 cctggcggcc ttcgccttcc tgcgcgacgc gatcccgccc gccatgaccc ggctgcacgc
     1081 cgccgcgcac agccccaccg gcgtcctggc cgagttgccg ctgctggcga tggacaccgg
     1141 ccccgccgcc gtcctgggca cgctcgaagt cccggcgctg cgcgccgccg ccatggcccc
     1201 ggcgggcgcg ctggtggtca acgtgggcaa cttccacacg ctggccttcc acctggccgg
     1261 gggcgccgtg gccgggctgt tcgagcacca caccggcgaa ctgaccgccg cccaactggc
     1321 cgggctgctg gaccggctgg ccgcgggcac gctgaccaac gacgaggtgt tccacacgca
     1381 gggccacggc gccctggtgc tggcgcccac cgcggcgccg ccggcgctct gcgccgtcac
     1441 cggcccgcgc cgcgccaaac tggccgccgc ctccctctcc tggccgctgc tgcccgccgt
     1501 gccccacggc gccatgatgc tgaccggctg cttcgccctc ctgcgcgccc tggcccacca
     1561 ctggcccccg gcccaggccc cggtcgccgc cgtgctaggg gcggacgagt ggccggtggt
     1621 tagtggtcag taagcggcca agtcgcgggc acaaatcagc aggggcgggt gtgctacccg
     1681 cccctgctgc tatatgcggt ctatcgtaac gactgaccac tggccactgg ccactgacca
     1741 ctcgtcacac gccgccctac cgttgcgtca ccgcgaagta gagcatcttc gagagttggc
     1801 cgcgggtgcc gttgttgccg gggcgatact ccaggcagcc cgtgccgcag gtgtaaccgg
     1861 agacgacccc cttggcgacc accgtctcga tgtgctggta gaagacgctg cccggcgcca
     1921 cgtcgcggaa gtgcgcggtg ggcggattga gcagcgtcca gcctttcgcc agcgcgatga
     1981 tcttggcgag ctggccgcgg gtga
//
LOCUS       DASUYC010000462         2332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_490930,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000462 DASUYC010000000
VERSION     DASUYC010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490930"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..748)
                     /locus_tag="VF276_07650"
     CDS             complement(131..748)
                     /locus_tag="VF276_07650"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015235551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX6606771.1"
                     /translation="MITLYTFGDSILDCGHYNEFGITPGGLLVQNDDRRFPEFAGQDL
                     RSRGPARLEHRARDGATVAALPAQARGLQVAGRTMALLTIGGNDLLQGLILDHGPGVE
                     AFAAALEAFLRALPIRPVLIGNVYDPSFGDNQRDFLGVDPTIARANHARANTVLAEMA
                     ERYGALVDLHAHFLLGDPSWFTQTIEPSLTGASEVRRCFLPFVLG"
     gene            complement(912..>2332)
                     /locus_tag="VF276_07655"
     CDS             complement(912..>2332)
                     /locus_tag="VF276_07655"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEX6606772.1"
                     /translation="DDPGKTVYPKITNVFYDDVSKHNIPQAFWDFLNQNGVVNVNGAV
                     RNERLINPWFYASGRPISEAYWANVKIAGRSTDVLIQAFERRVLTYVPTNTPDFQVEM
                     GNIGLHYRDWRYGGPGRVGNFPLSGPHPGYGFNVWLFDVDGNRVLQTAKNAGFNWVRQ
                     QVVWADYQQSPARINWARLDPVVNAAQANGVNLILSVVRSPGWAGQNGGMPTDKFTFA
                     GFMSQMASRYKGKVAAYEIWNEQNYAAESGGTVNLGQYVDLLQVGYMAVKYADPSAIV
                     MTGGLTPVGLNDPHIAVNDVEYLRQFYAYQGGVAKNYYDVLGAHPGSNNNSPDQKWPD
                     NPGTGHCPEIYRNLEGTCWRTDNSFYFRRIEDLRAVMEQAGEGNKQMWLTEFGWTTYN
                     TAPGYEYGQVISDQMQADYLRRAFEKGKNDYPWMGVMCVWNLNFSTLPWLPNSDEKVP
                     WSVLNRDFSPRPSYIALQQMPK"
BASE COUNT          349 a          755 c          802 g          426 t
ORIGIN      
        1 ccgcccggag gctcaagcct ccggctaccg ctatggaggc cgcctgcgcg gcctccattc
       61 agcccgcgca ggcgggcttt gtgctcgtag cccgcccctt ttaggggccg ggcgccgctg
      121 cgccgggcgc tcagcccagc acgaagggca ggaagcagcg ccgcacctcc gaggcgccgg
      181 tcaggcttgg ttcgatggtt tgcgtgaacc acgacggatc gcccagcagg aagtgggcgt
      241 gcagatcgac cagggcgccg tagcgttcgg ccatctccgc cagcacggtg ttcgcccgcg
      301 cgtggtttgc ccgcgcgatg gtgggatcga cgccgagaaa atcacgctga ttatcgccga
      361 agctggggtc atagacgttg ccgatcagca ccgggcggat gggcagcgcg cgcaagaagg
      421 cttccagcgc ggcggcgaac gcttccacgc ccggcccgtg gtccaggatc agcccttgca
      481 gcaggtcgtt gcctccgatg gtcagcaggg ccatcgtccg gccggcgacc tgcaacccac
      541 gcgcctgcgc cggcagcgcc gcgacggtcg ccccgtcgcg ggcgcggtgc tccaaccggg
      601 ccgggccgcg gctgcgtaga tcctggccgg cgaactccgg gaagcgccgg tcgtcgttct
      661 gcacgagcag cccaccaggc gtgatcccga actcgttata gtggccgcag tcgaggatcg
      721 agtccccgaa ggtgtacagc gtgatcatgc ttcgctcctg ccatagcata cctttagccc
      781 gttgccggtg tagcaccgct tcggcacaat ccggaccagc cccgcccggt gcggtgcaca
      841 aaaagagccg gggcggtgag acacgctcac cgccccggtg ttgctacgcc ttccgcgcgt
      901 ctaaatcgcg cttacttcgg catctgttgc aacgcgatgt acgaggggcg cgggctgaag
      961 tcgcggttga gcacgctcca cggcaccttc tcgtcgctgt tgggcagcca gggcagcgtg
     1021 ctgaagttga ggttccagac acacatcacg cccatccagg gatagtcgtt cttgcccttc
     1081 tcgaaggcgc ggcgcaggta atcggcctgc atctggtcgc tgatgacctg gccgtactcg
     1141 tagccgggcg ccgtgttgta ggtggtccag ccgaactcgg tcagccacat ctgcttgttg
     1201 ccctcgccgg cctgctccat gacggcgcgc aggtcttcga tgcggcggaa gtagaagctg
     1261 ttgtcggtgc gccagcaggt gccctccagg ttgcggtaga tctcggggca gtggcccgtg
     1321 ccgggattgt ccggccactt ctggtcgggc gagttgttgt tcgagccggg gtgcgcgccg
     1381 agcacgtcat agtagttctt ggcgacgccg ccctggtagg cgtagaactg gcgcaggtat
     1441 tccacgtcgt tgacggcgat gtgcgggtcg ttgaggccca cgggggtcag cccgccggtc
     1501 atgacgatgg cgctcgggtc ggcgtacttg acggccatgt agcccacctg tagcagatcg
     1561 acgtactggc ccaggttgac cgtgccgccg ctctcggcgg cgtagttctg ctcgttccag
     1621 atctcgtagg cggcgacctt gcctttatag cggctcgcca tctgcgacat gaagccggcg
     1681 aaggtgaact tgtcggtcgg catgccgccg ttctggccgg cccagccggg cgagcgcacc
     1741 acgctcagga tcaggttgac gccgttggcc tgcgccgcgt tgaccaccgg gtcgagccgc
     1801 gcccagttga tgcgggcggg gctctgctgg tagtcggccc agaccacctg ctggcgcacc
     1861 cagttgaaac ccgcgttctt cgccgtctgg agcacgcggt tgccgtccac gtcgaacagc
     1921 cagacgttaa agccatagcc ggggtgcggg ccgctcagcg ggaagttgcc cacgcgaccg
     1981 gggccgccgt agcgccagtc gcggtagtgc aggccgatgt tgcccatctc cacctggaag
     2041 tcgggcgtgt tcgtcggcac ataggtcagc acgcggcgct cgaacgcctg gatcagcacg
     2101 tcggtcgacc tgccggcgat cttgacgttg gcccagtagg cttcgctgat cgggcgcccg
     2161 ctggcgtaga accaggggtt gatcagccgc tcattgcgga cggcgccgtt gacgttgacc
     2221 acgccgttct ggttcaggaa gtcccagaag gcctgcggga tgttgtgctt ggagacatcg
     2281 tcgtagaaga cgttggtgat cttggggtag accgtcttgc cgggatcgtc gg
//
LOCUS       DASUYC010000463         3754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1949685, whole genome shotgun sequence.
ACCESSION   DASUYC010000463 DASUYC010000000
VERSION     DASUYC010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1949685"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VF276_07660"
     CDS             complement(<1..330)
                     /locus_tag="VF276_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012660508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX6606773.1"
                     /translation="MNDAASFGRWLRERRKALDLTQFDLAARVGCAEATIGRIEADER
                     RPSRQIAELLAVALGIPPDDRPAFIQFARIGTAAGTPAPGAEPARPAAPAPRPVIGAG
                     ALPLTPLI"
     gene            774..1307
                     /locus_tag="VF276_07665"
     CDS             774..1307
                     /locus_tag="VF276_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006667601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX6606774.1"
                     /translation="MAKLAAAVDERDHSLGPADAPVTLLVYGDYECPYTRKALLVVRQ
                     LREQSGDRLRFVFRNFPLTEIHPHAFHAAEAAEAAAAQGQDAFWAMHTYLFAHQQALE
                     DADLRQYAALLGLDTARFVQAMAGHTGASRINEDIESGLRSGVQGTPTLFINGTRHDG
                     SWDLGPLRTALARADQH"
     gene            1449..2576
                     /locus_tag="VF276_07670"
     CDS             1449..2576
                     /locus_tag="VF276_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007982425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEX6606775.1"
                     /translation="MMPTVNEQDIARYMANWQDEVDSAALYRAVAAAESNAQLAGVYQ
                     KLAAAEERHAAFWEGKLRDAGRPVPPRKIGWRTRVLGGLAKRFGPQFVLPTMNQMEQG
                     DVSSYDSQPEAAHTPLPADERSHARLLRTISGGLEGSAVAQLEGRHRATSGNALRAAV
                     LGANDGLLSNFSLVMGVAGAQLTGQAILITGLAGLLAGAGSMAMGEWISVQSSRELYT
                     RQIRVEAAEIEEVPDEEEEELALIYEAKGLPEAQARAMAARIMSDPATALDTLSREEL
                     GIDPQELGGSAYEAAITSFVLFAIGAIVPVAPFIFLAGWQAVVLSAVLSALALFGIGA
                     AITLMTGRSVLFSGMRQVAIGIAAAALTYALGSLLGVAIGG"
     gene            complement(2604..>3754)
                     /locus_tag="VF276_07675"
     CDS             complement(2604..>3754)
                     /locus_tag="VF276_07675"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX6606776.1"
                     /translation="LEGESLVNDATALVTYRTAVAAMAVAFSVGEASLRFVVVGVGGV
                     LVGLVVAFLVAALRRRIEDPSVEIALTLLTPFAAYLPAEQLGLSGVLATVAAGLYLGW
                     REPYITGSESRLGGRAVWQMVDFVLNGLVFILIGLQLSLILPALAGHLLIPLIGIGLL
                     VSLAMIVVRMAWIFGDNYLGGWLDRRKARGHGRPATGPALSRRETLVAGWAGMRGVVS
                     LAAALALPATTPERASLIFLTFLVILVTLVGQGLTLPLLIRALGVAQDGNGPAQQELR
                     ARRTVTEAALARITQLAGQWPTHLPLINALRAQYEHRASHLEDLPAEPEEESPADQEL
                     LEHHMIRRAVIDAERSAALHLRERGDIDDTIWRDIERELDLEELRMDA"
BASE COUNT          582 a         1260 c         1374 g          538 t
ORIGIN      
        1 gatcagcggc gtgagcggca aggccccggc gccgatcacg ggccgcgggg cgggcgctgc
       61 ggggcgcgcg ggttcggcgc cgggggctgg ggtcccggcg gcggtcccga tgcgggcaaa
      121 ctggatgaac gccgggcggt cgtcgggcgg gatgccgagg gccacggcga ggagttcggc
      181 gatctgccgc gagggccggc gctcgtcggc ttcgatccgg ccaatcgtcg cctcagcgca
      241 cccgacccgt gccgccaggt cgaactgggt caggtcgagg gctttgcggc gttcgcggag
      301 ccagcgccca aacgaggcgg cgtcattcat ggcgatcaag taccttttgc gcggcaccac
      361 ggcaaggctg ggacgttggg cacgatcggg ttggggactg cgctcatatt atacccgatc
      421 tttcgcttgt cgcgtcaagg ctcggcctgg cccaccggca gcgggccgaa accgcgctcc
      481 gcccgcggaa cgcagcggac cagggcgcgg caggcccggc ccgccccaat tgcccgccgt
      541 gcgccacagg ccaggggctg ggccgttgat cttaggaggg cgcggtcggg cggataataa
      601 cgcgcgacgg cgcaggaagg gaccgccgcg gccacggtgg ttgatcttag gagggcgcgg
      661 tcgggcggat aataaccgcc ttgaaccctc ggtttgcgcg ccaggcatag catggtccgg
      721 cggatctgct tggacatgcg cgccccacgc ggttcaagaa agagaggact gacatggcca
      781 agcttgctgc ggcggtcgac gaacgcgacc atagcctggg acccgcggat gcaccggtaa
      841 ctctgctggt gtatggggat tatgagtgcc cttacacgcg caaggcgctg ctggtggtgc
      901 ggcaactccg tgagcagtca ggggaccggc tgcgcttcgt gttccgcaac tttcccctca
      961 ccgagatcca cccgcacgcc ttccatgccg ctgaggccgc ggaagccgcc gcggcccagg
     1021 gccaggacgc gttctgggca atgcatacct atcttttcgc gcaccagcag gccctagaag
     1081 acgccgacct gcggcaatat gcggcgctgc tgggattgga taccgcgcga ttcgtgcagg
     1141 ccatggccgg gcacaccggc gcgtcccgga tcaacgagga tatcgaaagc gggttgcgga
     1201 gcggggttca gggcactccc accttgttca tcaacggtac gcgccacgac ggttcctggg
     1261 acctgggccc gctgcggacc gcgctggccc gcgccgacca gcattagcct gggatccgga
     1321 cgcgccaggg catagctgtt gctgtgttgc gccagggcca ggctgggggt gcgcgcaagc
     1381 ggccgcgcag cggccggtgg taccgcgcgg tgcgcgggat gatgaaagaa tgaggcaaac
     1441 gaggcaacat gatgccgact gtgaacgaac aggacatcgc ccgctatatg gcgaactggc
     1501 aggatgaggt ggacagcgcg gccctctacc gggcggtggc cgccgccgag tcgaacgccc
     1561 aactggccgg ggtataccag aaactcgccg ccgccgaaga gcggcacgcc gctttctggg
     1621 aaggcaagct gcgcgacgcg ggccggccgg tgccgccgcg caagatcggc tggcgcacgc
     1681 gggtgctggg cgggctggcg aagcgcttcg gcccgcagtt tgtgctgccc acgatgaatc
     1741 agatggagca gggcgatgtg agcagctacg acagccagcc cgaggcggcg catacgcccc
     1801 tgcccgccga cgaacgctcg cacgcccggc tgctgcgtac gatctccggc gggctggagg
     1861 gcagcgcggt ggcgcagttg gagggccgcc accgggccac cagcggcaac gccctgcggg
     1921 ccgccgtgct gggcgccaac gacggcctgc tctccaactt cagtctggtc atgggcgtgg
     1981 ccggcgcgca gttgaccggg caggccatcc tcatcaccgg gctggcgggc ctgctggccg
     2041 gggccgggtc gatggccatg ggcgagtgga tctcggtgca aagctcgcgc gaactctaca
     2101 cccgccagat ccgcgtggag gccgccgaga tcgaggaggt acccgacgag gaggaggagg
     2161 aactggccct gatctacgag gccaagggct tgcccgaggc ccaggcgcgg gccatggcgg
     2221 cgcgcatcat gtccgacccg gccacggcgc tcgatacgct ctcacgcgag gaactgggca
     2281 tcgacccgca ggaactgggc ggctcggcct acgaggcggc gatcacgtcg ttcgtgttgt
     2341 tcgccatcgg cgcgatcgtg cccgtggcgc cgttcatctt cctggcggga tggcaggccg
     2401 tggtgctcag cgcggtcttg agcgcgctgg cgctcttcgg catcggcgcg gccatcaccc
     2461 tgatgacggg ccgcagcgtg ctgttctcgg gtatgcgcca ggtcgccatc ggcatcgccg
     2521 ccgccgccct gacctacgcg ctcggctcgc ttctgggcgt cgccatcggc ggctagccgg
     2581 gcagcgcatc gcctgcgcct atgctaggcg tccatgcgca gctcttccag gtcgagttcg
     2641 cgctcgatgt cccgccagat cgtgtcgtcg atgtcgccgc gctcgcgcag atgcagcgcc
     2701 gccgagcgtt cggcatcgat caccgcccgg cggatcatgt ggtgttccag cagttcctgg
     2761 tccgccgggc tctcctcctc gggttccgcg ggcaggtcct ccaggtggct ggcgcgatgc
     2821 tcgtactggg cgcgcagggc gttgatcagc ggcaggtgcg tgggccactg gccggcgagt
     2881 tgggtgatgc gcgccagggc ggcctcggtc acggtgcgcc gggcgcggag ttcctgctgg
     2941 gccgggccgt tgccgtcctg ggccaccccc agcgcccgga tgagcaaggg caacgtgagc
     3001 ccctggccca ccagcgtcac caggatcacc aggaaggtca ggaagatcag cgaggcgcgt
     3061 tcgggcgtcg tcgcgggcaa ggcgagcgcc gccgccagcg agaccacccc gcgcatgccg
     3121 gcccatccgg cgacgagggt ctcgcgccgg cttaaggccg ggccggtggc cggccgcccg
     3181 tggccgcgcg ccttgcgccg gtcgagccac ccgcccaggt agttgtcccc gaagatccag
     3241 gccatgcgca ccacgatcat ggcgaggcta accagcaggc cgatgccgat cagcgggatc
     3301 agcaggtgcc cggccagcgc gggcaggatc agcgagagtt gcagcccgat gaggatgaac
     3361 accaggccgt tgagcacgaa gtcgaccatc tgccacacgg cgcgcccgcc gagccgcgat
     3421 tccgacccgg tgatatacgg ctcgcgccag cccaggtaca gcccggcggc cacggtggcc
     3481 agcacgccgg acaggccgag ttgttccgcc ggcaggtagg ccgcaaacgg cgtgagcagc
     3541 gtgagcgcga tttccaccga ggggtcttcg atgcgccggc gcagggcggc gacgaggaag
     3601 gccactacta atccgacgag caccccgccc acgccgacca cgacgaagcg gaggctcgct
     3661 tcacccaccg agaacgcgac ggccatggcc gccaccgcag tgcggtaggt gaccagggcg
     3721 gtggcgtcgt tgaccaggct ttcgccttcc agca
//
LOCUS       DASUYC010000464         4697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_489344,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000464 DASUYC010000000
VERSION     DASUYC010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4697
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489344"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="VF276_07680"
     CDS             <1..646
                     /locus_tag="VF276_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HEX6606777.1"
                     /translation="GIPLVGLKEPALRGGRLALKRAMDIVLAVAALVVLAPLLAVLAL
                     AIRLEKPHGPILFRQQRAGRGGKVFTCYKFRSMRPDAEALKAQLLAQNEASGPIFKMK
                     NDPRVTRVGRLLRRTSLDELPQLFNILRGDMSFVGPRPPIPSEVTAYSDWHLQRLEVT
                     PGLTGLWQVSGRSRLTFDEMVTLDLYYAENWSLLLDCRVLLQTLPAVLLQRGAY"
     gene            complement(706..1875)
                     /locus_tag="VF276_07685"
     CDS             complement(706..1875)
                     /locus_tag="VF276_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606778.1"
                     /translation="MSFLLVLLAGAGLANGAGAAPLNRDKPARTPGLTPVSDCTPDPR
                     GDLDAHITDHPDHTSAVFTNSSQHCSYQIGLATYMKVDDNIDHQIFYDGQTAVIAPES
                     TLTLQVGNPPCAYQGDAFWGRMLKSLEGGVRYGERRLDDTDGVNSTYCVVAATATPTQ
                     QAAPPTATATAAPPTATATSTPQVPTATATATAVPPSATATNTPTNTPTAMPPTATAT
                     NTPTPGAQKVRPTNTPTPIPAGSPPAPTATATAVTVVAPPAATSTPAVVPPTATTGAG
                     APPPATATATAVLAPPTVPAPAGGAPPAATAARTPAGVPGTTGGAPSAPPPAAPPGGQ
                     ETGPVSQVGPLVGSNAGPAPSELPRTGAPDLGWLPALVVAALALGSGLLLRRRAS"
     gene            2337..3755
                     /locus_tag="VF276_07690"
     CDS             2337..3755
                     /locus_tag="VF276_07690"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX6606779.1"
                     /translation="MAAEQATGATAERVGRIESAVLQEPIWRSQGGVPIPLAELMAQQ
                     GVAGVSIAVIHDGTLAWARGYGVREAGRPDPVTPETLFQVCSISKPVTAVAVLRLVQE
                     SRLDLDEDVNHYLRSWRVPANGDWQPRVTLRQLLCHGAGMTVHGFPGYGADAPRPTLR
                     QVLDGAPPANTAPVRVNALPGTQFRYAGGGTSIVQQVLMDVTGLPFAQLMRDLVLDPL
                     AMRDSTYEQPLPPTRQEAAATGHRSEGAPVTGKWHVYPEQAAAGLWTTPSDLARLAID
                     IQQARAGAPGTLLRPETVAQMLTPQVEEHIGLGFFLEGAGLDQRFAHGGDNEGYKANF
                     VAFSERGEGVIVATNGDLGWIVCDNLTRTVWREYGWPDFPGAPQVRTAFDAARAATCS
                     GRYELKPGYVLEVTVRDGALYLQATGQPAFALQPCGADEYFAEVVDAEVAFAVEDGQA
                     TKLIFKQNGKETPARRLDAMPG"
     gene            complement(3862..4338)
                     /locus_tag="VF276_07695"
     CDS             complement(3862..4338)
                     /locus_tag="VF276_07695"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6606780.1"
                     /translation="MSGTNLVIRPFTAADQAAARRLILDGLAEHFGWLDETLNPDLDD
                     IAAHYVARGALFVVAEIAGVPVGTGALVAEGPGGRLARISVAPAHRRRGVGRALVAHL
                     VGAAQARGWPQVLVETNDDWYDAIALYQRYGFREYTRAAGEVHMVLDLATPPAPGL"
BASE COUNT          736 a         1614 c         1660 g          687 t
ORIGIN      
        1 cggcatcccg ctggtcgggc tgaaggaacc ggccctgcgc ggcgggcgcc tggccctcaa
       61 gcgggccatg gatatcgtgc tggccgtagc cgcgctggtg gtgctggccc cgctgctggc
      121 cgtgctggcc ctggcgatcc gcctggagaa gccgcacggc ccgatcctgt tccgccagca
      181 gcgcgccggg cgcggcggca aagtcttcac ctgctacaag ttccgctcga tgcgccccga
      241 cgcggaggcg ttgaaggccc aactcctggc ccagaacgag gcgagcggcc ccatcttcaa
      301 gatgaagaac gacccgcgcg tcacgcgggt gggccgcctg ctgcgccgca ccagcctgga
      361 cgaactgccg cagttgttca acatcctgcg cggcgacatg agcttcgtcg gcccgcgccc
      421 gcccatcccc agcgaagtca ccgcgtacag cgactggcac ctgcagcggc tcgaagtgac
      481 gccggggctg acgggcctct ggcaggtcag cgggcgcagc cggctcacct tcgacgagat
      541 ggtgacgctc gacctctatt acgcggagaa ctggtcgctg ctgctcgact gccgggtgct
      601 gctgcaaacg ctgcccgccg tcctcctcca gcgcggcgcc tactagccgc cccaccaatc
      661 caggcaaaaa gagacccgcg ccggcttccg gcgcgggtct tggcgtcagg acgctctccg
      721 tcgcaacagc agcccgctgc ccagggccag cgcggccacc accagcgccg gcagccagcc
      781 gagatccggc gcgccggtgc gcggcagttc gctgggcgcg gggccggcgt tggaccccac
      841 cagcggcccg acctgactca ccggcccggt ctcctgcccg ccgggcggcg ctgcgggagg
      901 cggggcggag ggcgcgccac ccgttgtccc cggcacgcca gccggcgtgc gggcggccgt
      961 ggccgccggc ggcgccccgc cggccggtgc cggcactgtg ggcggcgcga ggacggcggt
     1021 cgccgtcgcc gtagcgggtg gcggggcgcc agccccggtg gtggccgtcg gcgggaccac
     1081 ggccggcgtc gatgtggccg ccggcggcgc cacgaccgtc acggcggtgg ccgtcgccgt
     1141 cggggcgggc gggctacccg ccgggatggg cgtgggagtg ttggtggggc gcaccttttg
     1201 cgctcccggc gtcggcgtgt tcgtcgccgt agccgtcggc ggcatggccg tcggcgtatt
     1261 cgtcggcgta ttcgtggccg tcgcgctcgg cgggacagcc gtggccgttg ccgtggcggt
     1321 gggcacttgc ggcgtgctcg tcgcggtagc ggtgggtggc gccgccgtgg ccgtcgccgt
     1381 cggcggggcc gcctgctgcg tcggcgtcgc ggtcgcggca acgacacagt aggtcgagtt
     1441 gacgccgtcg gtgtcgtcta accggcgctc gccatagcgc acgccgccct ccagcgactt
     1501 cagcatccgc ccccagaagg cgtcgccctg gtacgcgcac gggggattgc cgacctgcaa
     1561 cgtcagcgtg ctctccgggg cgattaccgc cgtctgcccg tcgtagaata tctggtggtc
     1621 gatgttgtcg tccaccttca tgtaggtcgc caggccgatc tgatagctgc aatgctgcga
     1681 actgttggtg aacaccgccg aagtgtgatc gggatgatcg gtgatgtggg catccaggtc
     1741 gccgcgcgga tcgggcgtgc agtcgctcac gggcgtgagg ccgggagtgc gcgccggttt
     1801 gtcgcggttg agcggggcgg cgccggcgcc gttcgctaga cccgcccccg ccagcaagac
     1861 tagaaggaaa gacaataaca gcaaccgtcc gccccgccgc gcacgcgggg cgtgaaaaaa
     1921 gggaaacatg ggaacacgac ctccggagtc tagataagta agatgtagta ggaagacgca
     1981 gacctaatgc aattccaatc cggcaaaggg tttgcgcgcc gggaggccaa ctgtcccacc
     2041 cgttctacta tctatgatag caagacctgt accatataca ctaaagaaat agatgaatct
     2101 taatatacgg taacctgata cctatattgc ctggttttgg tatccatcct gtaacggacg
     2161 gcacgatacg gggttgcaca cggtgcgatc gggcgcatag cccggcggcg ggccgggcca
     2221 agtctccacg cggatcgaga tacgcctctg gtccgttgcc gggggcgggc cgggtgtgct
     2281 acgctgtcgg caggcaagcg accccacgca tggctagctg caggaggaac gcacatatgg
     2341 ctgcggaaca ggcgacaggc gcaaccgccg agcgggtcgg gcgcatcgaa agcgcggtgt
     2401 tgcaggagcc catctggcgc agccagggcg gggtgcccat ccccctggcc gagttgatgg
     2461 cccagcaggg cgtggcgggc gtcagcatcg ccgtgatcca cgacggcacg ctggcgtggg
     2521 cgcgcggcta cggcgtccgc gaggccgggc ggcccgaccc ggtgacgccg gaaacgctgt
     2581 tccaggtctg ctcgatcagc aagccggtga ccgccgtggc ggtgctgcgg ctggtgcagg
     2641 agagccggct cgacctggac gaagatgtga accactactt gcgctcctgg cgcgtccccg
     2701 ccaacggcga ctggcagcca cgggtcacgc tgcgccagtt gctctgccac ggcgcgggga
     2761 tgacggtcca cggcttcccc ggctacggcg ccgatgcccc gcgccccacg ctgcgccagg
     2821 tgctcgacgg cgcgccgccc gccaacacgg cccccgtgcg cgtcaacgcc ctgcccggca
     2881 cccagttccg ctatgccggc ggcggcacca gcatcgtgca acaggtgttg atggacgtga
     2941 ccggcctgcc cttcgcccaa ctcatgcgcg acctggtact cgacccgctc gccatgcgcg
     3001 acagcaccta cgaacagccc ttacccccca cccggcagga ggccgcggcc accggccacc
     3061 gctccgaggg cgcccctgtg accggcaagt ggcacgtcta cccggaacag gccgcggcag
     3121 ggctgtggac cacccccagc gacctggctc gcctggcaat cgacatccag caggcgcggg
     3181 ccggcgcgcc cggcaccctc ttgcgaccgg agacggtggc gcagatgctc accccgcagg
     3241 tggaggaaca tatcgggctg ggcttcttcc tggaaggcgc gggcctggac cagcgtttcg
     3301 cccatggcgg cgacaacgag ggctataagg ccaacttcgt ggccttcagc gagcgcggcg
     3361 agggtgtgat cgtcgcgacc aacggcgacc tgggctggat cgtctgcgac aacctgacgc
     3421 gcacggtctg gcgggaatac ggctggcccg acttccccgg cgccccgcag gtccgcacgg
     3481 cgttcgatgc ggcgcgggcg gcgacctgct caggccgcta tgaactcaag ccgggctatg
     3541 tgctggaggt cacggtgcgc gacggcgcgc tctatttgca ggcgaccggg cagcccgcct
     3601 tcgcgctcca gccctgtggg gcggatgagt acttcgccga ggtggtggat gccgaggtag
     3661 ccttcgcggt ggaggacggc caggcgacga agctgatctt caagcagaac ggcaaggaga
     3721 cgcccgcgcg gcgcctggac gcaatgcctg gatgatctgc cgagtgcccg cccggttggg
     3781 gtataccccg caaggagcgg gcgagacaag aggagacagg cggcgcaggc ggccttcatt
     3841 catgcgaagc agcccgcccg ttcacaggcc gggcgcgggc ggggtcgcca gatcgagcac
     3901 catgtggact tcgccggcgg cgcgggtgta ttcgcggaag ccgtagcgct ggtagagcgc
     3961 aatcgcgtcg taccagtcgt cattcgtctc gaccagcacc tggggccagc cccgcgcctg
     4021 cgcggcgccc accaggtgcg cgaccagcgc gcgacccaca ccccggcgcc ggtgcgcggg
     4081 cgccaccgag atccgcgcca gccgcccgcc cggcccctcg gccaccagcg cccccgtgcc
     4141 caccggcacg ccggcgatct cggccacgac gaacagcgcc ccccgcgcga catagtgcgc
     4201 cgcgatatcg tcgagatccg ggttcagagt ctcgtccagc caaccgaaat gctcggccag
     4261 tccgtccagg atcagccggc gcgccgcggc ctgatcggcg gcggtaaatg ggcgaatgac
     4321 caaattagtg ccgctcacgg cgggaaacac ttgttcacga tggcatattt tatgcctcac
     4381 agctaccagg ctgagcaatg cgccgtcagg cgtgtgcgcg gcaggtgagc cggggggcat
     4441 acgaggacga ttacccggcg cgccaaccgg cgtgccttca attatgcagg tgcgtctcct
     4501 gaaagtcaat ggcaggcagc tgcgtgccgc ctatgcagcg gcaggttgct gtgaatcagg
     4561 cgccgccggc ccagaggatc ggcggcgcgg ctacaagggt gcaaagtaat ggcactgatc
     4621 aaggacgatt tcatccaggc ggcccagacc atcgtcgagg gtattaacga tatcctgggg
     4681 atgggcgcga tcgtcgt
//
LOCUS       DASUYC010000465         3250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1416417, whole genome shotgun sequence.
ACCESSION   DASUYC010000465 DASUYC010000000
VERSION     DASUYC010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1416417"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1360
                     /locus_tag="VF276_07700"
     CDS             <1..1360
                     /locus_tag="VF276_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606781.1"
                     /translation="AESYAAAAQWANAQAVYGIVAEATVEHYEEIEDEAGSLIDVLVT
                     CYEGLRQCLDAQADLPPEERLDPEARRMLIDALFTLWEHTLNFDLDVDEAIPTVIART
                     ATAEEKAEIERRLRRKLAEVPAETFDNSWRKRPIIAFLAELKGDGLSPDELLAEYRAA
                     GMYQELAAQLITMGRVDAAFEVAKEYLTSADQVTRFADVVLKADPARREQVLSFVETR
                     LGDAEWTQPSRQKTEWPHTRPDAYQITHELNQYREWLEARYIEFGLDEKALAMARRRF
                     DATPGAATYAAVKRAAYLPGLPADRWPAIRHELIVALKTQAAWPDLIRIYLDEQLPGE
                     ALQVLALLQARPAGGGIGSWRAGYVDGSLVTLEQQVAQAAEAEYPEQARDIYRRMAER
                     YIEARGRDNYIAAAELLVRVRELYLRLGQEAAWRAYIADLRQQHKSLRALKEELDSRA
                     LA"
     gene            complement(1369..2007)
                     /locus_tag="VF276_07705"
     CDS             complement(1369..2007)
                     /locus_tag="VF276_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019588592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6606782.1"
                     /translation="MPIRILLVDDHSVVRKGLRMFLSLDPELEVVGEARDGAEAVRMA
                     HELKPDVVLMDLLMPVMDGIQATATIRRELPDTEVLALTSVLEDSSVVGAVRAGAIGY
                     ILKDTEADELRRAIKAAAAGQVQLAPQAAERLLREVRTPESPEALTERETDVLRLVAK
                     GRANKEIAHELNIGEKTVKSHVSSILAKLNVPSRTQAALYAVRIGLVPPDAV"
     gene            2190..2564
                     /locus_tag="VF276_07710"
     CDS             2190..2564
                     /locus_tag="VF276_07710"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6606783.1"
                     /translation="MIRVLVVDDQPAIRRGLRMRLGLEDDLIVVGEAPDGPAALALAP
                     ALRPDVILLDVQMPGMDGIDTAEALRALAPACAVVLLTLHGDPITRARAEAAGVVVFV
                     EKHEADMALVQAIHRAAGAPRA"
     gene            complement(2607..2993)
                     /locus_tag="VF276_07715"
     CDS             complement(2607..2993)
                     /locus_tag="VF276_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HEX6606784.1"
                     /translation="MAKTIVHTDNAPQAGAYSQAVQAGGLVFVSGQGPFDPGTGAIVG
                     STIQEQTAQCLRNIAAILQAAGSSLDRAVSATFILADPADFAGMNEEWITWFPTDRPA
                     RQGAKLPIQVPGLRISIALVAEAAGT"
BASE COUNT          512 a         1061 c         1119 g          558 t
ORIGIN      
        1 ggccgagtcg tatgcggccg ccgcccagtg ggcgaacgcg caggctgtgt acggcatcgt
       61 cgcagaagca actgtcgagc attatgaaga gatcgaggat gaagcaggtt cgctaatcga
      121 tgtgcttgtc acctgttacg aaggcttgcg ccaatgcctt gatgcccagg cagacctccc
      181 gccggaggag cgcctggacc cggaggcgcg gcggatgctg atcgacgcgc tgttcaccct
      241 ctgggagcac acgctgaatt tcgacctgga tgtcgatgaa gcgatcccga cagtgatcgc
      301 gcgcacggcc acggcggagg agaaggccga gatcgagcgc cggttgcggc ggaagctggc
      361 ggaggtgccg gcggagacct ttgataactc ctggcgcaag cggcccatca tcgcgttcct
      421 ggccgagttg aagggcgacg gcctttcgcc ggacgagttg ctggccgagt accgggcggc
      481 gggcatgtac caggaactgg ccgcccagtt aatcaccatg gggcgggtgg acgccgcttt
      541 cgaggtcgcc aaggagtacc tgacgagcgc cgatcaggtc acccgcttcg ccgacgtggt
      601 gctgaaggcc gatccggcgc ggcgcgagca ggtgctgagc tttgtcgaga cgcgactcgg
      661 cgacgccgag tggacccagc cctcgcgcca gaagaccgag tggcctcata cgcgccccga
      721 tgcctaccag atcacccatg aactgaacca gtatcgtgag tggttggagg cacgctacat
      781 cgagtttggg ctggacgaga aggcgctggc gatggctcgc cgccgcttcg atgccacgcc
      841 gggcgcggcg acctatgccg cggtcaagcg cgccgcgtat ctgccgggcc tcccggcgga
      901 ccgatggccc gcgatccggc acgagttgat cgtcgcgctc aaaacgcaag ccgcctggcc
      961 cgatctaatt cgcatttacc tggacgaaca acttcccggc gaggcgctgc aggtgctggc
     1021 gctgttgcag gcccgcccgg cgggcggcgg gatcggttcc tggcgcgccg ggtatgtgga
     1081 tgggagcctg gtgaccctgg agcagcaggt ggcccaggcc gccgaggccg agtacccgga
     1141 gcaggcgcgc gacatttacc ggcgcatggc cgagcgctat atcgaggcgc gcgggcgcga
     1201 caattacatc gcggcggcgg aactgctggt gcgcgtgcgc gagttgtatc tgcggctggg
     1261 gcaggaagcg gcgtggcgag cctacatcgc cgatctccgg cagcagcaca agagcctgcg
     1321 cgcgctcaag gaggaactgg actcccgcgc gctggcctga gccggcgcct agaccgcgtc
     1381 gggggggacg aggccgatgc gcacggcgta gagggcggcc tgggtgcggc tgggcacgtt
     1441 gagcttggcg aggatgctgc tcacgtggct cttgacggtc ttctccccga tgttgagttc
     1501 gtgagcgatc tctttattgg cgcggccctt ggcgaccagg cgcagcacgt cggtttcgcg
     1561 ctccgtgagc gcctccgggc tttccggggt gcgcacttcg cgcagcaggc gctcggcggc
     1621 ctggggcgcc agttggactt gcccggcggc ggcggctttg atcgcccggc gcaattcgtc
     1681 ggcctcggtg tccttgagga tgtagccgat ggcgcccgcg cgcaccgcgc cgaccacgga
     1741 gctgtcttcc agcacactgg tcagggccag cacttccgtg tccggcagtt cgcggcggat
     1801 cgtggcggtt gcctggatgc cgtccatcac cggcatgagc aggtccatca gcacgacatc
     1861 gggcttgagc tcgtgcgcca tgcgcacggc ctcggccccg tcgcgcgcct cgccgaccac
     1921 ttcgagttcc gggtccaggc tgagaaacat ccgcaggccc ttgcgcacca cgctgtgatc
     1981 atccacgagt aagatccgaa tgggcatgct ttcctccgct atagggtgga gttccgtccc
     2041 gtccgaaagg cggacccgca aaggcatcat ttgcctgggg cgcgccgtgc ggccgcgcgc
     2101 tacactatct gaatatgcac cattatacca cagatcttac accatatcgg caccgcgttt
     2161 gcaccgggcg cgcggcgagg tacccgtcca tgatccgtgt attggtagtc gacgaccaac
     2221 cggccatccg ccggggcctg cgcatgcggc tgggtctgga ggatgatctg atcgtggtcg
     2281 gggaagcccc ggatgggccg gcggcgctcg cgctggcgcc cgcgttgcgg cccgatgtga
     2341 ttctgctcga tgtccagatg cccggcatgg acggtatcga caccgctgag gcgctgcggg
     2401 cgctagcccc cgcctgcgcc gtggtgctgc tgacgctgca cggcgacccg atcacccgcg
     2461 cgcgcgccga ggcggcgggc gtggtggtct tcgtcgagaa gcacgaggcc gacatggcgc
     2521 tggtccaggc cattcaccgg gccgcgggcg cgccccgcgc ttagagcctg gcggctatag
     2581 caatccgtga tctgcttcag gctagctcag gtccccgctg cctctgccac gagcgcgatg
     2641 gagatacgca ggccgggaac ctgaatcggg agtttcgctc cctggcgcgc gggccgatcg
     2701 gtggggaacc aggtgatcca ctcctcgttc atgccggcga aatccgccgg gtcggccaga
     2761 ataaaggtgg cgctcaccgc tcggtccaga gaactgcccg ccgcctgtag gatcgccgca
     2821 atattgcgta agcattgcgc ggtttgctcc tggatcgtgc tgccgacgat ggcgcccgtt
     2881 cctgggtcga aggggccttg cccggacacg aagaccagtc cgcccgcctg caccgcctgg
     2941 ctatacgccc ccgcctgggg cgcgttatcg gtatgcacga ttgtcttcgc catgtgcgac
     3001 ctccctcggg tgtggctcca tcacttgcga cccatcgtca ccccgctgct tacagcggat
     3061 gtgcaatccc ggcgcgtctt ctgagagctt agcacatccg tcgagccgca accgtatccc
     3121 acattacgcc agcgcgggcg tgtcctgctc atagctcgcc aggtcgaaga aggtctccag
     3181 ccgctcgccg tccagtgccc agatacgctc ggcgacgtgc tccacgaagt agcggtcgtg
     3241 gaccacggcc
//
LOCUS       DASUYC010000466         3154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1322897, whole genome shotgun sequence.
ACCESSION   DASUYC010000466 DASUYC010000000
VERSION     DASUYC010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322897"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="VF276_07720"
     CDS             <1..870
                     /locus_tag="VF276_07720"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEX6606785.1"
                     /translation="LAALMLSTGPGWTNATIVDRIQRTATDLGAPGRDDSYGYGLINT
                     AAAIANPPGHGPPPATATPPPVPPTAVPPTATRPPVPPTPRPVTPTPTIGPGCLFSDV
                     CPQTDWFGPFVQDLVNRGAVSGYGDNTFRPQVNITRGQVAKIVVLSGQIDLDTLSGPH
                     FRDVPPTHPLYAYIETAYARGIVVGYADGTFRPTTPITRGELAKMVVLARGWALDTPE
                     TPTFGDVPYGSPFYFYIETAANLQVISGYENGTFRPYANTTRAEAAKIIDVAAATFRP
                     GLGSAANSLAARP"
     gene            1045..1335
                     /locus_tag="VF276_07725"
     CDS             1045..1335
                     /locus_tag="VF276_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606786.1"
                     /translation="MPERVDEWDDEPAPVHKPVARPPVVPAAAVAPAPADAPAGLPDE
                     APPEAEDAADRHIPWRQAIANAPAWIWMIAIFCIGSICMMVGTVVLLIATFR"
     gene            1346..2890
                     /locus_tag="VF276_07730"
     CDS             1346..2890
                     /locus_tag="VF276_07730"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HEX6606787.1"
                     /translation="MADPSVAPPAAATVGGARRIARNIAGPVAAQLIGRFLTTAYAAA
                     MFRLMASPGQSNELQGYLIAALLTTYAGIITDWGLSTWLTREVAAHRGDADALAKTRT
                     YFAETLAARLGLAAVASLALLIVAATPALSGVFQLSASGATALVLLGLALLPGAFSSA
                     VTALYNAYERMSLPAWLTVLTAFVNLMLGVGALVLGWGAPGLGAASLLTTTVTAFLFY
                     RLLWRDFFAPTLRWQTGAVRQMLVVAFPLMVNGLLMFIFFRFDSFIINHYRPQDVPIY
                     EAAYKFINVTQILPPIVVLAFFPAMARAAIHDKAALARHYRMAVKLMLLIALPMVAGT
                     VVLAGPLITIFTLGKPGYLPYAAWALAILICYLPFSFINGVTQYVLIALNRQGRLTWV
                     IGAAALFNIGANLLAVPAFGIYGAAAVTVLTELVLLAPFLGWAGADLGMAVLRPGPAG
                     IKLVLAGGALTAATAGLSAVGLGGLPAVVGGGLAYGAALGTLRIFSPAEWAVMRSVVQ
                     RRRAAA"
BASE COUNT          422 a         1139 c         1049 g          544 t
ORIGIN      
        1 ctggccgccc tcatgctcag cacggggccg gggtggacca atgcgacgat tgtcgaccgc
       61 atccagcgca cggcgaccga cctgggcgcg ccgggccgcg acgactccta cggctacggg
      121 ttgatcaata cggcggcggc catcgccaat ccgcccggcc acgggccgcc cccggccacc
      181 gcgaccccgc ccccggtccc gcccaccgcc gtgccgccga cggcgacgcg cccgcccgtg
      241 ccgcccacgc cgcgcccggt cacgcccacg ccgaccatcg ggccgggctg cctcttctcg
      301 gatgtctgcc cgcaaaccga ctggttcggc cccttcgtgc aggacctggt gaaccggggc
      361 gcggtcagcg gctacggcga caacaccttc cgcccgcagg tgaatatcac gcgcggccag
      421 gtcgccaaga tcgtcgtgct gtccgggcag atcgacctcg acacactgag cggaccgcac
      481 ttccgcgacg tgccgcccac ccacccgctc tatgcgtaca tcgagacggc ctacgcgcgg
      541 ggcatcgtcg tgggctacgc cgacggcacc ttccggccca ccacgcccat cacgcggggc
      601 gaactggcga agatggttgt gctggcgcgc ggctgggcgc tcgatacgcc ggagacgccc
      661 acgtttggcg atgtgcccta cggcagcccc ttctacttct atatcgagac ggcggcgaac
      721 ctgcaagtca tctccggtta cgagaacggc accttccggc cctatgccaa cacgacacgt
      781 gctgaggcgg ccaagatcat cgacgtggcc gccgccacct tccggcccgg tctcggcagt
      841 gcggcgaaca gcctggccgc ccggccctga taccgagttc accgcgaaga cgcgaagagc
      901 gcgaagatcg tttgtaaaag tcatactctt cgtgtccttc gcgtcttcgc ggtgccgaac
      961 aagcgccggc tttgacgcgg gtcggtggcg cccggtataa tacgggagtc gcgcgaatac
     1021 cagtgccgga aagggaacag ggagatgcct gagcgggttg acgaatggga tgacgagccg
     1081 gcgccggttc acaagccggt ggcccggccc cctgtcgtgc ccgccgccgc cgtggcgccg
     1141 gcgcccgccg atgcccccgc cggcttgccg gacgaggccc cgccagaggc cgaggacgcc
     1201 gccgaccggc acatcccctg gcggcaggcc atcgcgaacg ccccggcctg gatctggatg
     1261 atcgcgatct tctgcatcgg ctcgatttgc atgatggtgg ggacggtggt tctgctcatt
     1321 gcaaccttca ggtaaccccg cgccggtggc cgacccgtct gtcgccccgc cggctgccgc
     1381 gaccgtgggc ggggcgcggc gcatcgcgcg caacatcgcc ggcccggtcg ccgcccagct
     1441 tatcggtcgc ttcctgacca cggcttatgc cgccgccatg ttccggctga tggcctcgcc
     1501 cggccagtcg aacgaactgc aaggctacct catcgccgcg ctgctcacca cctacgccgg
     1561 gatcatcacc gattggggtc tgagcacctg gctcacgcgc gaggtcgccg cgcatcgggg
     1621 cgacgccgac gcgctggcga agacgcggac ctatttcgcg gaaacgcttg cggcgcggtt
     1681 agggctggcg gccgtcgcca gcctcgccct gctgatcgtg gcggccacgc cggccctgtc
     1741 cggggtgttc cagctttcgg catccggggc caccgcgctg gtgctgctgg ggctggcgct
     1801 gctgcccggc gccttctcca gcgccgtgac cgccctctac aacgcctacg agcgcatgag
     1861 cctgccggcc tggctgacgg tgctcacggc cttcgtcaac ctgatgctgg gggtcggcgc
     1921 gctggtgctg ggctggggcg cgccgggcct gggcgctgct tcgctgctga cgaccaccgt
     1981 gacggctttc ctgttctacc gcctgctgtg gcgcgacttc ttcgccccca ccctccgctg
     2041 gcagacgggc gccgtccgcc agatgctcgt cgtggccttc ccgctgatgg tcaacgggtt
     2101 gctgatgttc atctttttcc gcttcgactc gtttatcatc aaccactacc ggccacagga
     2161 tgtgccgatc tacgaagcgg cgtacaagtt catcaacgtc acccagattt tgccgccgat
     2221 tgtcgtgctg gcctttttcc ctgccatggc gcgcgccgcg atccacgata aggccgccct
     2281 ggcgcggcac tatcgcatgg cggtcaagct catgctgctg atcgctctgc cgatggtcgc
     2341 gggcacggtg gtgctggccg ggccgctgat cacgatcttc acgttgggca agccaggcta
     2401 cctgccctat gccgcgtggg cgctggcgat cctgatctgc tacctgccct tcagcttcat
     2461 caacggggtc acgcagtatg tgctgatcgc gctcaaccgc caggggcggc tgacctgggt
     2521 catcggggcg gcagcgctgt tcaacatcgg ggccaacttg ctggccgtgc cggccttcgg
     2581 catctacggc gccgccgcgg tgacggtgct caccgaactg gtgttgctgg cgcccttcct
     2641 gggctgggcg ggtgccgacc tggggatggc cgtgctgcga ccggggccgg cggggatcaa
     2701 actggtgctg gccggcggcg cgctgaccgc cgccacggcg gggttgagcg cggtagggct
     2761 gggcgggcta ccggcggtgg tgggcggcgg gctggcctat ggcgcggcgc tgggtacctt
     2821 acgcatcttc agcccggcgg agtgggccgt aatgcgcagc gtggtccagc gccgccgggc
     2881 tgccgcgtag aggaacgacg aagcgtccag gtaacggtta aggacggcct cccttgccgt
     2941 tgccgccacc attgccgcca ccgctattcc ctccaccgtt cccgccgccg ttgccgccgc
     3001 cgctattccc tccaccgttc cccccgccat tgccgttacc attgccatta ccgttgccgt
     3061 tcccgttgcc gttgccatta ccgttgccgt tcccgttgtt ggcaggcgtg ggggacacgg
     3121 caggcggggg cgtgttggtg ggcggagcgg cttg
//
LOCUS       DASUYC010000467         3271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_24555,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000467 DASUYC010000000
VERSION     DASUYC010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24555"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1655
                     /locus_tag="VF276_07735"
     CDS             <1..1655
                     /locus_tag="VF276_07735"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX6606788.1"
                     /translation="MTKETEAVTVLRNVRAYTMDAGQPEAEALAWRGDRLIAVGEADT
                     VVAAAGARAQLIDGGGRTVVPGLIDSHIHFMGYAANLLHIELDGVSSLETALDRVATR
                     TATARAGAWVRGRGWNNNLWSPTAFPTRHDLDRVTGDHPALMTRKDGHSIWVNSAALA
                     LTGITRDTPDPPGGRIGRDAQGEPDGMLYEGAAMAGVYRLLPDDSPEDIRRAVQQALQ
                     NATAAGLTGFHDCESPEAFAAFQQLEAAGQLPLRVVMLLALPTLRESIAVGLRSGFGS
                     TRLRVGPVKIFSDGSLGSQTAEMLAPFEGTTDNYGVGTIAQADLEAAILAASGAGIAV
                     AVHAIGDAANRRVLDAFARARLVESGGVPPPGGADGDGYARYTLRHRIEHAQMVDPAD
                     WGRFRELGVIASMQPIHATSDMEAADRLWGARVGRGGYAWRSLQQAGALLAFGSDCPV
                     ESFDPLLGIHAAVTRQDTHNQPPGGWYPEERLPVADAVRAYTLDAAFAGGMDHLLGSL
                     TPGKLADFVLLDRDIFTADPQTISQTRVTATVVGGDVVAGAL"
     gene            1814..>3271
                     /locus_tag="VF276_07740"
     CDS             1814..>3271
                     /locus_tag="VF276_07740"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6606789.1"
                     /translation="MADHPSGTVTFLFTDIEGSTKLWEHHPEAMRVALARHDDLLRAA
                     IENGAGYVFKTVGDAFCAAFWTAQDGLAAALAAQRALLAEPWGATGPIRVRMALHTGA
                     TEERDGDYFGPPVNRVARLLSAGHGGQTLVSGPTAELLRDHLNDGIRLRDMGECRLKD
                     LVRPERVFQVLAPDLADTFPALKTLDSRPNNLPVQPTALIGREREAAAAAALLRRPDV
                     RLLSFTGPGGTGKTRLSLQVAADLLDDFADGVFFVPLAPISDPALVGSTIAGVLGVKE
                     APGQALDAGLKAFVHDKQLLLVLDNFEQVVPAAPLVATLLAGSPGLKALVTSREVLRV
                     YGERDFPVPPLALPDPHRLPPLEQLTQYEAVRLFITRAQAVKADFQVTNDNAPAVAEI
                     CARLDGLPLAIELAAARSRLLPPQALLTRLTSRLKLLTGGGRDLPARQQTLRGAVDWS
                     YDLLEPGEQALFSRLALFVGGCTFEAAEAVCNAGGD"
BASE COUNT          472 a         1171 c         1182 g          446 t
ORIGIN      
        1 acatgacaaa ggaaaccgaa gcggtaaccg tgttgcgcaa tgtgcgcgcg tatacgatgg
       61 atgccgggca gccggaggcg gaggccctgg cctggcgcgg cgaccggctg atcgccgtgg
      121 gcgaggccga tacggtggtg gcggcggcgg gggcgcgggc gcaactgatc gacggtgggg
      181 gccgcacggt agtgcctggt ctcatcgaca gccatatcca cttcatgggc tatgccgcca
      241 acctgctgca catcgagctg gacggcgtgt ccagcctcga gaccgcgctg gaccgcgtcg
      301 ccacgcgcac ggcgacggcg cgcgccgggg cctgggtgcg cgggcgcggc tggaacaaca
      361 acctctggtc gccgacggcc ttccccacgc ggcatgacct ggaccgcgtg accggcgacc
      421 acccggcgct catgacgcgc aaggacggcc actcgatctg ggtgaacagc gcggcgctgg
      481 ccctgaccgg catcacccgc gacacgcccg acccgccggg tgggcgcatc gggcgcgacg
      541 cgcagggcga gcccgacggc atgctctacg agggcgcggc catggccggc gtctaccgcc
      601 tgctgccgga tgacagccca gaagacatcc gccgcgccgt ccagcaggcc ctgcagaacg
      661 ccactgccgc cgggctgacc ggcttccacg actgcgagtc gccggaggcg ttcgccgcct
      721 tccagcagtt ggaggcggcg gggcagttgc cgctgcgcgt ggtcatgctg ctggcgctgc
      781 ccaccctgcg cgaaagcatc gccgtggggc tgcgcagcgg cttcggcagc acccgcctgc
      841 gcgtgggccc ggtgaagatc ttcagcgacg gcagcctggg gtcacagacc gccgagatgc
      901 tggccccctt cgagggcacc accgacaact acggcgtggg gacgatcgcg caggccgact
      961 tggaggccgc catcctggcg gcgagcgggg cgggcatcgc cgtggccgtc catgccatcg
     1021 gcgacgcggc caaccggcgc gtgctcgacg ccttcgcccg cgcccgcctg gtcgaatccg
     1081 gcggcgtacc cccgcccggc ggcgccgatg gcgacggcta cgcccgctac acactgcgcc
     1141 accgcatcga gcacgcccag atggtcgacc ccgccgactg gggccgcttc cgcgaactgg
     1201 gcgtgatcgc ctcgatgcag ccgatccacg cgacctccga tatggaggcc gccgaccggt
     1261 tgtggggcgc gcgggtgggc cggggcggct atgcctggcg cagcttgcag caggcgggcg
     1321 ccctcctggc tttcggttcg gattgcccgg tggagagttt cgacccgctg ctcggcatcc
     1381 acgccgccgt cacccggcag gacacgcaca accagccgcc gggcgggtgg tacccggaag
     1441 agcggttgcc ggtggccgat gcggtgcgcg cctacacgct cgacgcggcc ttcgcgggcg
     1501 gcatggatca cctgctcggc tcgctcaccc ccggcaagct ggccgacttc gtcctgctcg
     1561 accgggacat cttcaccgcc gacccgcaga ccatctccca gacgcgggtc acggccacgg
     1621 tggtgggtgg agacgtagtg gcgggggcgc tgtagcgata tgcgccgtgg cgctgcccgc
     1681 ggatgccggc gccacggcgt actcctggcc gcccgacggc aatataactc aatataacta
     1741 tagttacgcg aatggcggat ggcggcctgc cgccgggagc aggccggcag ggacagatac
     1801 gaggaggggc cgggtggcgg atcatcccag cgggacggtg acgttcctgt ttaccgatat
     1861 cgaaggcagc accaagctct gggaacacca ccccgaagcg atgcgcgtgg cgctggcgcg
     1921 gcacgacgac ctgctgcgcg cggccatcga aaacggggcc ggctacgtct tcaagacggt
     1981 cggcgatgcc ttctgcgccg cgttctggac cgcgcaggat gggctggccg ccgccctggc
     2041 cgcgcaacgg gcgctgctgg ccgagccctg gggggcgacc gggccgatcc gcgtgcgcat
     2101 ggccctgcac accggtgcga ccgaggagcg cgacggcgac tacttcggac cgccggtcaa
     2161 ccgggtggcg cgcctgctgt cggcgggcca cggcgggcaa acgctggtat ccggccccac
     2221 cgccgaactg ctgcgcgacc acctcaacga cggtatccgc ctgcgcgaca tgggcgagtg
     2281 ccggctcaaa gacctggtgc ggcccgagcg ggtcttccag gtgctggcgc ccgatctggc
     2341 cgacaccttc ccggcgctca agacgctgga cagccgcccc aacaacctgc cggtgcagcc
     2401 caccgcgttg atcgggcgcg agcgcgaggc ggcggcggcg gcggcgctct tgcgccggcc
     2461 cgatgtgcgc ctgctgagct ttaccgggcc gggcggcacc ggcaagaccc ggttgagcct
     2521 gcaagtggcc gccgacctgc tcgacgactt cgccgacggc gtcttctttg tcccgctcgc
     2581 gccgatcagc gacccggccc tggttggctc gacgattgcg ggagtgctgg gcgtgaagga
     2641 ggcgccggga caggcgctcg acgcgggcct caaggcgttt gtccacgaca agcaactcct
     2701 gctggtgctc gacaacttcg agcaagtggt gcccgccgcg ccgctggtgg cgaccctgct
     2761 ggcgggcagt ccgggcctca aggccctggt gacgagccgg gaggtgctgc gggtctacgg
     2821 cgagcgcgat tttccggtgc cgccgctggc tctgcccgat ccccaccgcc tgccgccgct
     2881 cgaacaactg acgcaatacg aggctgtgcg cctgttcatc acgcgggcgc aggcggtcaa
     2941 ggccgacttc caggtgacga acgacaacgc gcccgccgtg gccgaaatct gcgcccggct
     3001 cgacggcctg cccctggcga tcgaactggc cgcagcgcgc agccggttgc tgccgccgca
     3061 ggccctgctg acccggctga ccagccgcct gaagctgctc accggcggcg ggcgcgacct
     3121 gcccgcccgc cagcaaacgc tgcgcggcgc ggtcgattgg agctacgatc tgctcgaacc
     3181 cggcgagcag gccctgttca gccggttggc gctgttcgtc ggcggctgca ccttcgaggc
     3241 ggcggaagcc gtctgcaacg ccgggggcga c
//
LOCUS       DASUYC010000468         2253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1446981, whole genome shotgun sequence.
ACCESSION   DASUYC010000468 DASUYC010000000
VERSION     DASUYC010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446981"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="VF276_07745"
     CDS             <1..793
                     /locus_tag="VF276_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HEX6606790.1"
                     /translation="PELQAGGARHQQLRHAARQEIGIRRFLETQRAKAWTTTFEDLHG
                     LNQLPGLAAQRLMNDGYGYGGEGDWKTAALVRAMIVMASGLEGGTSFMEDYTYHLDPA
                     GMKILGSHMLEISEQITAQRPRLEVHPLSIGGKADPVRLVFDARTGPAVAASIVDMGN
                     RFRMVVNTVDAVPLDQPLPKLPVARALWLPHPDLQTAATAWILAGGAHHTGFSYALTA
                     EHLADFAQMAGIEFLLIDRDTKLADFKKELRWNDLYYHLARGLQG"
     gene            1366..>2253
                     /locus_tag="VF276_07750"
     CDS             1366..>2253
                     /locus_tag="VF276_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606791.1"
                     /translation="MAEQAAPPAPLVHERIYRRNFVFFLTDGILFMLAISLIGSTTVI
                     PDFVRRLTDSQVLIGLSGSLFELGWTLPQLFIARLIVRAERKKWWFVGPNIPVRGLIL
                     LFAGLTLLLGPTHPEILLPAFFVCYGLAALGDGLVGVPWADLAGSSLDHRWRARLFGV
                     QNAVTGVLLLGLAPVIALILGQLAFPANYGLLFGLAGALFALSSLPMLFIQELPGGPA
                     QETIPPLGEFLPDLVQVLRTDGPFRAIVLTRMLTSLLAMAGPFYIGFATGPLALSSDL
                     AVPTLLAVQTVGSIGGAVGY"
BASE COUNT          354 a          753 c          724 g          422 t
ORIGIN      
        1 cccggagctg caggcgggcg gcgcccggca tcagcagctg cgccacgcag cccggcagga
       61 gatcggcatt cgccgctttc tggagacgca gcgggccaaa gcctggacca ccaccttcga
      121 ggacctgcac ggcctgaacc agttgccggg cctggcggcg caacggctga tgaacgacgg
      181 ctatggctac ggcggggagg gcgactggaa aacggcggcg ctggtgcgcg cgatgatagt
      241 catggcgagc ggcctggagg ggggcacatc cttcatggag gattatacct atcacctcga
      301 cccggcgggc atgaagatcc tcggttcgca catgctcgag atctccgagc agattaccgc
      361 ccagcggccc cgcctggagg tgcatccgct gtccatcggc ggcaaggccg acccggtgcg
      421 cctggtgttc gacgcccgga cggggccggc ggtggccgcc tccatcgtgg atatgggcaa
      481 tcgcttccgc atggtggtca acaccgtgga tgcggtgccg ctggaccagc cgctgccgaa
      541 gctcccggta gcgcgggcgc tctggctgcc ccatccggat ctgcagaccg ccgccacggc
      601 ctggatcctg gcgggcggcg cccatcatac cgggtttagc tatgcgctga cggcggaaca
      661 cctcgcggat ttcgcccaga tggccggcat cgagtttctg ctgatcgacc gggacacgaa
      721 gctggcggac ttcaagaaag aactgcgctg gaacgacctg tactaccatc ttgcgcgggg
      781 cctgcagggg tgagccgctc gtcacgggcg gccggaacta tatcggcagt attgtcaccg
      841 cggaattgct gcaggccggc catgaagtaa tggtctttgc tgatttcgcc ggcccgcagc
      901 cggccgccgt cccgcccgcg gtcactctcg tcatgcgcgc gcaaggttaa cgctacggtt
      961 atccggggta taaactccgt gacaaactac ggcacacccg tcgcggatgc cgccggcctg
     1021 tcgaacagag cgccggcggc ctctgcgccg gatattctac ggtggcaatc cccggagcct
     1081 ccctggttct gcgaggcgcc ggcccgcact agaagggaaa cgtggtataa tgacccgacg
     1141 aaagacctgg aagggaaccg gctctgttac aggatgatct ctatggcctg caggctccat
     1201 cgccgttact cccgcgtgtg ccatggggag cagaaacacc gagtggcttc cggccgcatc
     1261 gccctctcgt tacccttcga tgcggcggat tgggcaatca atgctgctgc aagtattcgc
     1321 tgcacgggcc gggcgccggg ctgatccctc gtgaacgaag ccgtcatggc cgaacaggcc
     1381 gcgccgcccg cccccctggt ccacgaacgc atctaccggc gcaatttcgt cttcttcctc
     1441 accgacggca tcttattcat gctcgccatc agcctgattg gcagcacgac ggtcattccc
     1501 gactttgtcc gccgcctgac cgactcgcaa gtcttgatcg gcctctccgg tagtctgttt
     1561 gaactgggct ggacgctgcc gcagttgttc atcgcccgcc tgatcgtccg cgctgagcgc
     1621 aaaaaatggt ggtttgtggg ccccaatatt ccggtgcgcg ggctcatctt gctgttcgcc
     1681 ggactgaccc tgctcctcgg tcccacccac ccggagatcc tcttacccgc ctttttcgtc
     1741 tgctacggcc tggcggcgct gggcgatggg ctcgtcgggg tgccctgggc cgacctcgcc
     1801 ggcagcagcc tcgaccaccg ctggcgggcc cgcctgtttg gcgtgcagaa tgcagtcacc
     1861 ggggtcctgt tgctaggact cgccccggtg atcgcgctga tcctcgggca attggcattt
     1921 cccgccaatt atggcctgct ctttggcctc gccggcgcct tattcgccct gtccagcctg
     1981 cccatgctgt tcatccagga actgccggga gggccggcgc aagagacgat tccgccacta
     2041 ggcgaatttc tgccggacct ggtgcaggtg ctgcgcaccg atgggccgtt tcgcgcgatc
     2101 gtgctgaccc ggatgctcac cagcttgctg gcgatggccg gcccgtttta catcggcttc
     2161 gcaaccgggc cgttagccct gtccagcgac ctggcggtac cgacgctgct ggccgtgcag
     2221 acggtgggca gcatcggcgg ggcagtgggg tac
//
LOCUS       DASUYC010000469         4297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1841807, whole genome shotgun sequence.
ACCESSION   DASUYC010000469 DASUYC010000000
VERSION     DASUYC010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4297
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1841807"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="VF276_07755"
     CDS             complement(<1..874)
                     /locus_tag="VF276_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX6606792.1"
                     /translation="MLKTLRGRLTASYVAVVLAALVLATVAYFALGVRYRTEDAYRQL
                     ATSMYLVAPQVEDALGPGLGLLDRARVLGATEHIRGELRNTRFRLLLLRPDGRVVLDS
                     LTGRGNMTGRLMDDLPAALVSGPVPSEPVDGLLRADAAGNAWIYVAAPPRAVIGNGVA
                     GAGGNPRFYMLLAAQRPSFADVAQSYTSRFLLVGLLAVGVSLVVGGLLARSITRPLGT
                     LAAASHAIAGGEYGHQAPVTGSAEMRALAADFNYMAGQVQRAQQAQRDFLANISHDLK
                     TPLTSIQGFSQAMLD"
     gene            complement(1265..1957)
                     /locus_tag="VF276_07760"
     CDS             complement(1265..1957)
                     /locus_tag="VF276_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6606793.1"
                     /translation="MSEKTILVVDDERHIVDLVKLYLEAEGFRVEAAYDGNAALRLAR
                     TLRPVLIVLDLMLPGLDGFEVCRTLRRESDVPILMLTARADDVDKIVGLELGADDYLA
                     KPFNPRELQARVKAILRRTAAPAPGAAAAIVLGNTVIDPARREVTIAGEPVELRSKEF
                     DLLKMFAQYPGLVLDRERLLNQVWGYDYFGGTRTVDVHIAHLRERLIESTLEIQTVRG
                     SGYKLVVLGGGR"
     gene            2473..4251
                     /locus_tag="VF276_07765"
     CDS             2473..4251
                     /locus_tag="VF276_07765"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX6606794.1"
                     /translation="MLKRVSSDPNNLDNLSADLIEEGSPVLLNGLLTLLRWLIAGAAV
                     ATLGALAQVGARPIPWQDPVPATLIVLYVLGAALVFLTSYLLPRQSYWLVDVGLLFDL
                     TVLIALSLATGGATAVLIVPPLFAIGILRNIWAGLLAGVLFGLHLIPIWPPAHELAIL
                     PQSSQAVLLGLYTILGLVWVSLVAGGNAQWVRKHARDARQGEKRALDRYHLALRRAKG
                     IYHVTYTLSATLNYESVLRAIFEEFVRVLPFDVGMVLLIEPGSNLFYVSGSYGLGKDE
                     VDRKLDARTGRLGRAAASGTPVIVNDLAHEDELIACWPRLANFSSALILPLRSRFELY
                     GVVVTAVEKGKYDEDQLELLSALGNNAIIALQNAQLYQALREERDRLLGKEDEVRRQL
                     ARDLHDGPAQTLAAIAMNAEFIKKLLASAPDRVPGELDELADMARKANQDVRTLLFEL
                     RPVILESSGLVTALSQYVNRFPTTDLPRVHFQGEEFDLRLAPAAENTVFNIVQESINN
                     AKKHARARNIWVSLRRISNKLIIVIQDDGQGFDVSLQSDQATKRGSYGLLNMRERAAA
                     VNGDIGITSVRGRGTTITLSVPLLAN"
BASE COUNT          715 a         1531 c         1388 g          663 t
ORIGIN      
        1 cgtcgagcat ggcctgagag aagccctgga ttgaggtcag cggggtcttg aggtcatgcg
       61 agatgttcgc caggaagtcg cgctgggcct gctgcgcccg ctgcacctgc ccggccatat
      121 agttgaagtc ggccgccagg gcgcgcatct cggcgctgcc ggtgacgggc gcctggtggc
      181 cgtactcgcc cccggcgatg gcgtggctgg cggcggccag cgtgcccagc ggccgggtga
      241 tcgagcgggc cagcagcccc ccgaccacca gcgatacacc cacggccagc agccccacca
      301 gcaggaagcg cgaggtgtag ctctgcgcca catcggcgaa gctcggtcgt tgcgcggcca
      361 gcagcatata gaagcgcggg ttgccgcccg cccccgccac cccgttgccg atgacggccc
      421 gcggcggcgc ggcgacatag atccaggcgt tgcccgccgc gtcggcccgg agcagcccgt
      481 ctacgggttc gctgggcacg gggccgctga ccagcgccgc gggcaggtcg tccatgagcc
      541 ggcccgtcat gttgccccgt ccggtcaggc tgtcgagcac cacgcgcccg tcgggccgca
      601 gcagcagcag gcgaaagcgc gtattgcgca gctcgccgcg gatatgctcg gtggcgccca
      661 gcacccgcgc ccggtccagc agacccaggc ccggccccag ggcgtcctcc acctgcggcg
      721 ccaccaggta catgctcgtc gccaactggc ggtaggcgtc ctcggtgcga tagcgcacgc
      781 cgagcgcgaa ataggccacc gtcgccagca ccagcgcggc cagcacgacg gcgacatagc
      841 tcgcggtcag ccgcccgcgg agggttttca gcatagcgta tcctcaccgc gcacgcgcgc
      901 cggcccagcc cgccgccggg cacccgcgct gcgctcccct cattatagcc gcccggcatt
      961 agcgcccgat taaccccgcg taaaatcccg gtaaaatccg ccgccgcccg ccgcccacga
     1021 cccgatccgc gatagtggcc ccgcccggct cctaaaggag cgggctagtg ctacgaagcc
     1081 cgcctgcgcg ggctggggta ggaccgctcg tctgcgcgcc acccgacgag acgagactct
     1141 tcgctccgct cagagtgaca agccactcgc cgttctgttc atcgactcac tcatctcctg
     1201 acggctgacc gctgacggct gaccactgac cactgaccac tgaccaccga ccactgacca
     1261 ctcgtcaccg cccgccgccg agcaccacca gcttgtagcc gctgccgcgc accgtctgga
     1321 tctccagggt ggactcgatc aggcgctcgc gcaggtgggc gatatgcacg tcgaccgtgc
     1381 gcgtgccgcc gaaatagtcg tagccccaga cctggttgag cagccgctcg cggtcgagca
     1441 ccaggccagg gtattgcgcg aacatcttga ggaggtcgaa ctccttgctg cgcaactcca
     1501 ccggctcccc ggcaatcgtc acctcgcgcc gcgccgggtc gatgaccgtg ttgcccagca
     1561 caatcgccgc ggcggcgccg ggcgcggggg ccgccgtccg gcgcagaatg gccttgaccc
     1621 gcgcctgcaa ttcgcgcggg ttaaagggct tcgccaggta atcgtcggcg cccagttcga
     1681 gcccgacgat cttatccacg tcgtcggcgc gggcggtcag catcaggata ggcacgtcgc
     1741 tctcgcggcg cagggtgcgg cagacctcga acccgtcgag accgggcagc atcaagtcga
     1801 gcacgatcag cactgggcgc agcgtacgcg cgaggcgcag ggccgcgttg ccgtcgtagg
     1861 cggcttccac ccgaaaaccc tcggcctcca gatacaattt caccagatcg acaatatggc
     1921 gctcgtcgtc cacgacgaga atcgtctttt cgctcacggc tgcatggcct cctgcctgat
     1981 tatagcgcag ccccggcagc cgggcaacgc gggccggatg cacaccagcg gggcagaggg
     2041 cgcagagggt ttcgggggtt cattttcacc gcgaaggaac gaagggcgcg aagagttggg
     2101 ggtttggatg tttctggaag ccgctaatac catgccggac accccttaga atccttcgtt
     2161 gccttcgttc cttcgcggtg aatttcaacc ctctgcgccg ctgcggtgaa cattcggaga
     2221 agggtggaaa ccgggccggg atcgtagcaa attggtcctg ttagggtccc aggaccgggg
     2281 gtgaagggtt gcgtgcgggt aaaagtgctg gtacaatgaa cccaggggtg ccgattagcg
     2341 atcctccgat cccaacatgc aggaccccct ccgcggggca acggcacccg gacccaacgc
     2401 acaaatgtgg cgcgatgaat caccggcggc acctcccgcc ggccaccccg aactctgcgg
     2461 ggatcaaaag tagtgctgaa gcgagtttct tccgatccga acaatctgga taatctgtcc
     2521 gccgacctca tcgaggaagg cagcccggtc ctgctcaacg gcctgctgac gctgttgcgc
     2581 tggctgatcg cgggcgccgc ggtcgccacg ctgggcgcgc tggcccaggt gggcgcccgg
     2641 cccatcccct ggcaggaccc ggtgccggcc acgctcatcg tcctctatgt gctcggcgcc
     2701 gccctggtct ttctcaccag ctatctgctg ccgcgccagt cctactggct ggtggacgtg
     2761 ggcctgctgt tcgacctcac cgtcttgatc gccctgtcgc tggcgacggg cggggccacg
     2821 gcggtgctga tcgtgccgcc gctgttcgcc atcggcatcc tgcgcaacat ctgggcgggg
     2881 ctgctggcgg gcgtgttgtt cggcctgcac ctgatcccca tctggccgcc ggcccacgaa
     2941 ctggcgatcc tgccccagtc gagccaggcg gtgctgctgg ggctgtacac cattctgggg
     3001 ctggtctggg tgagcctggt ggcgggcggc aacgcgcagt gggtgcgcaa gcacgcccgc
     3061 gatgcccgcc agggcgaaaa gcgcgccctc gatcgctacc acctggcgct gcgccgggcc
     3121 aagggcatct accacgtcac ctatacgctg agcgccacgc tcaactacga aagcgtgctg
     3181 cgcgccatct tcgaggaatt cgtgcgcgtg ctgccgttcg acgtgggcat ggtgctgttg
     3241 atcgagccgg gcagcaacct gttctatgtc tccgggtcgt atggcctggg caaggacgag
     3301 gtggaccgca agctcgacgc gcgcaccggg cgcctgggcc gcgccgccgc cagcggcacc
     3361 ccggtcatcg tcaacgacct ggcccacgag gacgagttga tcgcctgctg gccgcggctg
     3421 gcgaacttca gcagcgcgct gatcctgccc ctgcgctcgc gctttgagct ctacggcgtc
     3481 gtcgtcacgg cggtcgagaa gggcaagtac gacgaggacc agttggaact gctgagcgcg
     3541 ctcggcaaca acgctatcat cgccctgcaa aacgcccagc tttaccaggc gctgcgcgaa
     3601 gagcgcgacc ggctgctggg caaggaggac gaggtgcgcc gccaactggc ccgcgacctc
     3661 catgacggcc cggcccagac gctggccgcc atcgccatga acgccgagtt catcaagaaa
     3721 ctgctcgcca gcgcgcccga ccgcgtgccc ggcgaactcg acgaactggc cgacatggcc
     3781 cgcaaagcca accaggacgt gcgcaccctg ctgttcgagc tgcgccccgt gatcctggag
     3841 agcagcggcc tggtcacggc ccttagccag tatgtgaacc gctttccgac caccgacctg
     3901 ccgcgggtcc acttccaggg cgaggagttc gatctgcgcc tggcccccgc cgccgagaac
     3961 acggtcttca acatcgtgca ggagtcgatc aacaacgcca agaagcacgc ccgcgcccgc
     4021 aacatctggg tcagcctgcg ccgcatcagc aacaagctga tcatcgtgat ccaggacgac
     4081 gggcagggct tcgacgtgag cctgcaaagc gaccaggcca ccaagcgcgg ctcctacggc
     4141 ctgctgaaca tgcgcgaacg ggctgccgcc gtcaacggcg acatcggcat cacctcggtc
     4201 cgcgggcgcg gcaccaccat caccctgtcg gtgccgctgc tcgcgaactg atcagctatc
     4261 agccgtcagc cgtcagccgt cagccgtcag ccgtcag
//
LOCUS       DASUYC010000470         2071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1862060, whole genome shotgun sequence.
ACCESSION   DASUYC010000470 DASUYC010000000
VERSION     DASUYC010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1862060"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(316..1287)
                     /locus_tag="VF276_07770"
     CDS             complement(316..1287)
                     /locus_tag="VF276_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEX6606795.1"
                     /translation="MAPATPPPSPAAPTGGPLRVLGSTDLALGGGTSRFDYQSLDPQT
                     RLLFIAHLGASLVTVFDTRTSRVVADIPDIASVHGVLAVPSLGRVYASATGADQVAVI
                     DEQTLKVVARIPGGSYPDGLAYDPAGKQVFVSDESGATDTVIDTATNSRRATIPLGGE
                     AGNTQYDPVSGQMFVAVQSRNQLVALDPATNKVLGRTPLPGCEHDHGLLIDAPARRAF
                     VACDGNARLLTLDLNAMRILDTQSVGDAPDVLALDPGWHRLYVAAESGVLSIFDAGGS
                     VPRKLREGVFAPAAHSVAVDPATHRVYLPLANVSGRAVLRVVQYSPP"
BASE COUNT          317 a          690 c          738 g          326 t
ORIGIN      
        1 cgatgcagct aattcattat ggccgaaagg gcgccagggg agggagtagc gggcggtgcg
       61 ccggttgccg cgattggaga ggatcctctt cggaaccaga tcccgcacat cgcgatacgc
      121 ggccagactc gctacggcaa agcggccccg gcgccgctcc cggtcctgcc ggtgcttgcc
      181 tgggccgcgt gcacgcctac ccccaggccg gcgaacagaa tggcgtgcac ggatatcctc
      241 gcgatcctgc tcaggccgcc gcccttgccg gtatgctgct ccgttgaggg gtgctgcccg
      301 gcctcccgtg cgccgctacg gcggcgagta ctgcactaca cgcaacacgg ctctaccgct
      361 cacgttcgcc aggggcagat aaaccctgtg cgtggccgga tctaccgcca cggagtgggc
      421 cgccggcgca aacaccccct cgcggagctt gcgcggcacg ctgccccctg cgtcgaagat
      481 cgacagcacc ccgctctccg ccgcgacgta gagccggtgc cagccagggt cgagcgccag
      541 cacgtcgggc gcgtccccta cgctctgcgt gtccagtatc cgcatggcgt ttaggtccaa
      601 cgtcagcaat cgggcgttgc cgtcgcaggc cacgaaggcc cgccgcgccg gcgcgtcgat
      661 cagcagcccg tggtcgtgct cgcagccggg tagcggcgtg cgccccagga ccttgttcgt
      721 ggccgggtcg agggccacga gctgattgcg cgattgaacg gccacgaaca tctggcccga
      781 aaccgggtcg tactgggtgt tgccggcctc cccgccgagc ggtatggtcg ccctgcggct
      841 gttggtcgcg gtgtcgatca ctgtgtcggt ggcaccgctc tcgtcggaga cgaagacctg
      901 tttgccggca gggtcgtagg cgagcccgtc ggggtagctg ccgccgggga tgcgggcgac
      961 gaccttgagc gtctgctcgt cgatgacggc cacctggtcg gcgcctgtgg ccgaggcgta
     1021 gacccgcccc aaggagggca cggcgaggac gccatgcacg ctggcgatat cgggtatatc
     1081 ggcgacgacc cggctggttc gggtatcgaa cacggtgacc aggctggcgc ccaggtgggc
     1141 gatgaacagc agcctggttt gcgggtccag gctctggtaa tcgaagcgcg acgtgcctcc
     1201 gccgagcgcg aggtcggtgc tgcccagcac acggagcggc ccgccagtcg gagcggcggg
     1261 tgacggcggc ggggtggccg gcgccatggg cgccccggca gcgggagggg gcggcctgac
     1321 cggcgcaccg gccgtggcgg ccggggcaga cgagctttga tcggctgtcc gggtcgcggc
     1381 tgccgccggc gccggggcgc cgccacgggt tgcagcggga gccggcgcgg tacccgccgg
     1441 ccctaccgtg gggctgttac gagtcaccgc gcacgcggtg gcgccgggac tcgccggcga
     1501 taacagggcg ccggccggtg ccggcgttgc ggcggccggc gcgggcgacg ttgccgccgc
     1561 cccgccacac gcgctgagac aggccaggcc aagcagtgcc gccccacaaa ccaggccgcg
     1621 gtagagacac aatttcatca gctctctttc acggctggcc gcgcgtgttt cttaaccacg
     1681 atccaaaatc agcccgatca gcgaggcgcc gagcacgccg gagcgccgga aacggctctg
     1741 cactggtctc tctgctcgcc tgatgctttt accgtaattg gcgcggctta tcgggcacaa
     1801 tgccactcca ccgtcttcac cgacaagcgg tccctggccg gcaacgacag ggtatcgtag
     1861 tcttgcctcg cgtagggcga ctcggcaacg aataagacga ccagacacta tccagtatcc
     1921 gctggctcta gtgtaacaac ggttacctga aattgcaacg cggatcgcga tgctggttct
     1981 ggtgctatcg cggcgggggg ctggcgaatc cggttttgtc gggccggaag gctgtaggtt
     2041 aggggagggt ttgcgaatgg actggatcac a
//
LOCUS       DASUYC010000471         2197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1662696, whole genome shotgun sequence.
ACCESSION   DASUYC010000471 DASUYC010000000
VERSION     DASUYC010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1662696"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1116
                     /locus_tag="VF276_07775"
     CDS             <1..1116
                     /locus_tag="VF276_07775"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6606796.1"
                     /translation="TPGAPGPGEEDAMVVREGDLPIRAFDPAHATEAEWAALNRFYNA
                     LQAETDPDDEPQSLDQTQGWMLHSEPTTHRKFWVVPAPGGDEIVAAGRVETWDTDDNR
                     HLADFYIEVRPAYRRRGLARRLLAPIAQEVRDRGRRLLRSWTVERIPAGAGFMASLGA
                     TLGQEGHENQLRLAGVDRAMLARWQAQARERAAGFEIGLWDGRYPEDQLEAIAELHHS
                     TNLAPRGDLDEEDVIMTPQRLREGDDANEQQGYSRWSIYAREIATGKLAGFTELYWHP
                     DQPTLAWQGWTAVWPQYRNKGLGRWIKAAMIEKLIAERPQVTRVRTENADSNAPMLQI
                     NYELGFRPYQRSDVWQVPLDTVEQYLAARQAEAGRIS"
     gene            1283..1486
                     /locus_tag="VF276_07780"
     CDS             1283..1486
                     /locus_tag="VF276_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606797.1"
                     /translation="MRKIFSYIFFLLGLLVMVLSILNIFNLLDLQVDLWVAVAMAAFG
                     LILCLIAFFMLAPEGGANPDELA"
     gene            1717..1860
                     /locus_tag="VF276_07785"
     CDS             1717..1860
                     /locus_tag="VF276_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606798.1"
                     /translation="MHTLFASGIIEKIIKAVRDLADAVTPRPTPVPVPVPVRSPQPRL
                     ERR"
BASE COUNT          421 a          753 c          690 g          333 t
ORIGIN      
        1 acgccgggcg caccgggtcc gggggaggaa gatgccatgg ttgtccggga gggtgatcta
       61 cccatccgcg cgtttgaccc cgcgcacgcc accgaagccg agtgggccgc gctgaaccgc
      121 ttctacaacg cgctgcaagc cgaaaccgac cccgacgacg agccgcaaag cctggatcaa
      181 acccagggct ggatgctcca ctccgagccg accacccacc gcaagttctg ggtggtgccc
      241 gcgccgggcg gcgacgagat cgtggccgcc gggcgggtcg agacctggga cacggacgac
      301 aaccgccatc tggccgactt ctacatcgag gtgcgccccg cctaccgccg ccggggcctc
      361 gcgcggcggc tgctggcgcc catcgcccag gaggtgcgcg accggggccg ccgcctgctg
      421 cgcagttgga ccgtcgagcg catccccgca ggcgcgggct tcatggcaag cctgggcgcc
      481 acgctgggcc aggaaggcca cgagaaccag ctccgcctgg ccggcgtgga ccgcgccatg
      541 ctggcgcgct ggcaggccca ggcgcgcgaa cgcgcggcgg gcttcgagat cggcctatgg
      601 gacggccgct accccgaaga ccaactcgaa gccatcgccg aactccacca ctccaccaac
      661 ctcgcgcccc gcggcgacct ggacgaagaa gacgtgatca tgaccccgca acgcctgcgc
      721 gaaggcgacg acgccaacga acagcagggc tactcgcgct ggagcatcta cgcccgcgag
      781 atcgccaccg gcaagctggc cgggtttacc gagctctact ggcaccccga ccagccgacg
      841 ctggcctggc agggctggac cgccgtgtgg ccgcaatacc gcaacaaggg cctgggccgc
      901 tggatcaagg cggccatgat cgaaaagctg atcgccgagc gcccgcaggt gacgcgcgtg
      961 cgcaccgaga acgccgactc caacgcgccc atgttgcaga tcaactacga actcggcttc
     1021 cggccttatc agagatccga cgtctggcag gtgccgctcg atacggtcga acaatacctc
     1081 gctgcccgcc aagccgaagc cgggcggatt tcctgagagc aggggccgtg cgccacgcac
     1141 ggccccgccc gattccccgc gaaaaaccgc cttctttccc ctcgcgacgg gtgctcatcg
     1201 cggcctgatc tctgctataa taatggtaga acgtacccgc ccggaacggc ggccgggggc
     1261 gagcgagagg agatggtggg caatgcgcaa aatcttcagc tatatcttct tcctgttggg
     1321 cctgctggtc atggtcctca gcatcctgaa tatcttcaac ctcctcgatc tccaggtcga
     1381 cctgtgggtg gccgtcgcca tggccgcgtt cgggctgatt ctctgcctga tcgccttctt
     1441 catgctggcg ccggagggcg gggccaaccc ggacgaactg gcctagcgcc gggcactagc
     1501 ccgaaggtcc tgggactctg gcctaaaagt cccaaagggg gcggcaaggg cttgcacggt
     1561 tcttgcttat tagctacaat atgcttatgc cccccgcggg ttcagaaacc ggcccgccgg
     1621 atggggcaaa acgcaaaacc tttcggaggc ggagcgcgtt tataataatg agtgcccggt
     1681 tacgatatat gccacggcgc cgaggagttt agagtcatgc acacgttgtt tgcaagcggc
     1741 atcatcgaga aaatcatcaa agctgtccgc gacctcgccg acgccgtgac gccccgcccc
     1801 accccggtcc ctgtgcccgt gccggtgcgg tcaccgcaac cccggctcga acggcgctga
     1861 cctagcgatc acagggccgc gcgccccaat aataacgcgc aatcacgagg ctcccgccag
     1921 gcgcgggagc ctctgctgtt tttcccccaa ctcacggcat agcccttgca cggcatccgg
     1981 taaaaccaga gcgtccgggt aggccgtata gggtatagca cccgtgcacc acgttgagga
     2041 ggagatcgtt gataacccaa tgggtccgag tagcggcggc agccgacttg ccgccgggag
     2101 aaaagatggc cgtagcagtc gcaggagaag aaatcgcgct gttcaattta gacggcaccg
     2161 tgtacgccat tggcgacatc tgcacgcatg ccgaagc
//
LOCUS       DASUYC010000472         4346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_778863,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000472 DASUYC010000000
VERSION     DASUYC010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4346
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778863"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VF276_07790"
     CDS             <1..282
                     /locus_tag="VF276_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606799.1"
                     /translation="VPQPTVTPAVPTATPTVPVPQPTVTPAVPTATPTVPVPQPTVTP
                     GIPTVTPTVPVPLPTVTPVGPTNTPTVPAPPLQGPGNNLTRNLTAPRGN"
     gene            437..>4346
                     /locus_tag="VF276_07795"
     CDS             437..>4346
                     /locus_tag="VF276_07795"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HEX6606800.1"
                     /translation="MALTFSEDDNAWRGNGSNSYVDLGNPSYLNFTGPITIEAWINLT
                     DGTGIRDIVAHGYSFSPNGEVFLRINNGRFQAGSWDGADHLAEAPMYSTDVGRWIHLA
                     AVYDGTTWALVRDGAYAGRSAPTTGPVPVNAPWAIGARGGGGERSFAGRIRNVAIWNT
                     ARNLNQIDADRIGTPNFADPHLLGFWALDEGTSGATAFDYTPHGLTGSFMNGSWAYPY
                     ALEFNGSNAFVDLGNPAPLNIQGAITLLAWVKPYTLDGMRNILAHGYRRDPNAEVYLR
                     IINGQYQVGAWNGTDHAVVASIPREDVGHWVCLCGTYDGYTWRLYRNGELVGQAADPV
                     GAIAMDAPWAIGARGGGGERFFQGQIRHAGVWNRAISPLEAWATFRSRILSSGDGSLV
                     GYWPGTDGSGPQAVDHSRVPNPNTIHNAAWVEVKDPLPDTQMQPTGAEQFVSYNDHAL
                     ALDGTSTYVDLGAAPSLNIQGAITMQAWLKPAATDGLRDILAHGYTFNPAGEVFLRLA
                     NGQYQVGSWDGTDHYAGGAVTADDLQRWTHICGVYDGSAWILYRNGQMIGRSAPTTGP
                     VAVNAPWAIGARGGGGDRYFAGQLRHVAVWNRALDPATIAAHANTQDMLDITEAGLVG
                     YWPLGEGTGTSAVNAVNPTANGTIHNAAWQDVLSTHGQPYLVSQARLMQDYRPDPGHP
                     GQTIEISGYRTVLSARAATGAPLRTNLTLWGTAPALLHFTDGTTAVLDTIRPLTRATN
                     AQGELSFVIEAQGQLTTPLIKVRADFMGDNERLLVAPDRQAHQQLGMITGAQLRGLAP
                     PPGALDLRPAVVPASVTQAQADAAAGAMRMILTTVVNHDVQPTVAQVASRDLGGASAP
                     LPPPRPYIPPYMQAERDLGGPPNPLRDDQPMHMLPVDQPVRRVLNVGSMGTTPWVLDL
                     RAGTFTQQADAGTRGLDGGEMVYLDDASRMLDGLAPLGAASFISLGQTRGIFDDISNF
                     FTHTIPDAANTAAQETSNFFNNTLPATATTVANQVAGGVTGAYNTVEQQTTAAFQQAG
                     DAIETGGTIVFSAVSTTIQQAGQLAEQVITGIRVVVNTVINGVQKAFSLVLQTVEDAG
                     RFVGTVLDSLGTTVQNLINYLKSLFNWGDIVSTQLVIQDALLQIQRVIREAIAVLAQG
                     QFVDDAIARLKTLINNNFDSWITALTGHAAPVNTDGNAPPQDVQSKYLQSLFNDNISR
                     TGGGLDAFQLSQEVIAVAQQLATRLQAAGGTLFSAETFQNSGLNDLLQHPEHLLTLQG
                     IADLLGIIKPLLNAGLDIAGAVIHAMLELMGIVLNALLDLF"
BASE COUNT          744 a         1665 c         1320 g          617 t
ORIGIN      
        1 gtcccgcagc cgaccgtcac gccggcggtc ccgaccgcca cgccgacggt gcccgtcccg
       61 cagccgaccg tcacgccggc ggtcccgacc gccacgccga ccgtgcctgt cccacagccg
      121 accgtcacgc cggggatccc aaccgttacg ccgaccgtgc ctgtgccgct gccgactgtc
      181 acgccggtgg gacccaccaa cacgccaacc gtgcccgccc cgcccctgca ggggccgggc
      241 aacaacctga cccgtaacct taccgcccca cggggcaatt gactctgccg ggaaacaggt
      301 gtaggctggg gatgccgtcc caaggatgta tttgcaccct cgatgatgta ccctcccgca
      361 gaaaaataag tagcgctggg gaacgcggcc cgtaagactc cccctccgcc caactcccga
      421 aaggagaatc cccaaagtgg ctttgacgtt ttcagaagac gacaacgcat ggcgtggtaa
      481 cggttccaat tcctatgtag acctcggcaa tcccagttac ctcaatttca ccggccccat
      541 caccatcgag gcctggatca acctcaccga cggcaccggc atccgcgaca ttgtcgccca
      601 cggctacagc tttagtccca atggtgaggt cttcctgcgc atcaacaacg gccgcttcca
      661 ggccgggtcc tgggacggcg ccgaccatct ggccgaggcg ccgatgtact cgaccgacgt
      721 gggccgctgg atccacctgg ccgccgtgta tgacggcacc acctgggccc tggtgcgcga
      781 cggggcctac gccggccgct ccgcgcccac gaccgggccg gtgccggtga acgcgccgtg
      841 ggccatcggg gcgcgcggcg gcggcggcga acgctccttc gccgggcgca tccgcaacgt
      901 cgccatctgg aataccgccc gcaacctgaa ccagatcgac gccgatcgga tcggcacgcc
      961 caacttcgcc gacccccacc tgctcggctt ctgggcgctc gacgagggca ccagcggcgc
     1021 cacggcgttc gactacacgc cgcacgggct gaccggcagc tttatgaacg gctcgtgggc
     1081 ctacccctac gcgctggagt tcaacggctc caacgccttt gtggacctgg gcaaccccgc
     1141 gccgctgaac atccagggcg ccattactct gctggcctgg gtcaagccct acacgctcga
     1201 cggcatgcgc aacatcctgg cccacggcta ccggcgggat ccgaatgccg aggtctacct
     1261 gcggatcatc aacggccagt atcaggtcgg cgcctggaac ggcaccgatc atgcggtggt
     1321 ggcgtccatc ccccgcgagg atgtgggcca ctgggtctgc ctctgcggca cctatgacgg
     1381 ctacacctgg cggctttacc gcaacggcga actcgtcggc caggcggcgg accccgtcgg
     1441 ggccatcgcc atggacgcgc cgtgggccat cggggcgcgc ggcggcgggg gcgagcgctt
     1501 cttccagggc cagatccgcc acgccggcgt ctggaaccgg gccatcagcc cactcgaagc
     1561 ctgggcgacc ttccgctccc gtatcctgtc gtcgggcgac ggtagcctgg tcggctactg
     1621 gcccggcacc gacggcagcg gcccgcaggc cgtcgatcac tcgcgcgtgc ccaaccccaa
     1681 caccattcac aatgccgcct gggtcgaggt caaggacccg ctgcccgaca cgcagatgca
     1741 gcccacaggc gccgagcagt tcgtcagcta taacgaccac gcgctggccc tcgacggcac
     1801 tagcacctat gtggacctgg gcgccgcgcc ctcgctgaac atccaggggg cgattacgat
     1861 gcaggcgtgg ctcaagcccg cggccaccga cggcctgcgc gatattctgg cccacggcta
     1921 caccttcaat cccgccggag aggtcttcct gcggctcgcc aacggccagt accaggtcgg
     1981 ctcatgggac ggcaccgacc attacgccgg gggcgcggtc accgccgacg acttgcagcg
     2041 ctggacccat atttgcggcg tctatgacgg cagcgcctgg attctctatc gcaacgggca
     2101 gatgatcggg cgctcggcac ccaccacggg gccggtcgca gtgaatgcgc cgtgggccat
     2161 cggggcgcgc ggcggcggcg gcgaccgcta tttcgccggg caactacgcc atgtcgctgt
     2221 ctggaaccgc gccctcgacc cggccaccat cgcggcccac gccaataccc aggacatgct
     2281 cgacatcacc gaggccgggc tggtcggcta ctggccgctc ggcgagggca ccggcacgtc
     2341 tgccgtcaac gcggtcaacc cgacagccaa cggcacgatc cacaacgccg cctggcagga
     2401 tgtgctcagc acccacggcc agccttatct cgtctcgcaa gcccggctga tgcaggacta
     2461 tcgccccgac cccggccatc cgggccagac catcgagatc agcggctacc gcaccgtgtt
     2521 gtcggcgcgc gcggcgacgg gcgcacccct gcgcaccaac ctgaccctgt gggggacggc
     2581 cccggccctg ctccacttca ccgacggcac caccgctgtg cttgacacca tccgcccgct
     2641 cacccgcgcc accaacgcgc agggcgaact cagcttcgtc atcgaggccc agggccaact
     2701 gacgaccccg ctgatcaagg tccgcgccga cttcatgggt gacaacgagc ggctgctggt
     2761 ggcgcccgac cggcaggcgc accagcaact cggcatgatc accggcgccc aactgcgcgg
     2821 cctggccccg ccccccggcg cgctggacct ccgccccgcc gtcgtgcccg ccagcgtcac
     2881 ccaggcgcag gccgatgccg ccgccggcgc catgcgtatg atcctgacca ccgtggtcaa
     2941 ccacgacgtg cagcccaccg tcgcgcaggt ggcgagccgc gacctgggcg gtgcgagcgc
     3001 cccgctgccg ccgccccggc cctacatccc gccctacatg caggccgaac gtgacctggg
     3061 cggcccgccc aacccgctgc gcgacgatca gccgatgcac atgctgccgg tcgaccagcc
     3121 ggtgcgccgc gtgctcaacg tcggcagcat gggcaccaca ccctgggtgc tcgacctgcg
     3181 cgccgggacg ttcacccagc aggcggatgc gggcacccgc ggcctcgacg gtggggagat
     3241 ggtttacctg gacgacgcca gccgcatgct cgacggcctg gctccgctcg gcgccgcctc
     3301 gttcatcagc ctgggccaga cccgcggcat cttcgacgac atcagcaact tcttcaccca
     3361 caccatcccc gacgcggcca acaccgccgc ccaggaaacc tcgaacttct tcaacaatac
     3421 cctgcccgcc accgccacga ccgttgctaa ccaggtggcc ggcggcgtca ccggcgccta
     3481 caacacggtc gaacagcaga ccacggcggc cttccagcag gcgggcgacg ccatcgagac
     3541 cggcggcacg atcgtgttca gcgccgtctc cacgaccatc cagcaggccg ggcaactggc
     3601 cgagcaggtc atcaccggca tccgcgtcgt cgtcaacacc gtcatcaacg gggtgcagaa
     3661 agccttctcc ctggtgctgc aaacggtgga ggacgccggg cgcttcgtgg gcacggtgct
     3721 cgacagcctg ggcaccaccg tgcagaacct gatcaactat ctcaagtcgc tgttcaactg
     3781 gggcgacatc gtctccaccc agctcgtcat ccaggacgcg ctgctgcaga tccagcgcgt
     3841 gatccgcgag gccatcgccg tgctggccca gggccagttc gtggacgacg ccatcgcccg
     3901 gctcaagacg ctgatcaaca ataacttcga ctcctggatc accgcgctga ccggccacgc
     3961 cgcgccggtg aacaccgacg gcaacgcgcc gccgcaagac gtgcagagca agtatctgca
     4021 atcgctgttc aacgacaaca tctcgcgcac cggcggcggg ctggacgcct tccaactgag
     4081 ccaggaggtg atcgccgtgg cccagcaact ggcgacccgg ttgcaggcgg cgggcggcac
     4141 cctgttctcc gccgagacgt tccagaactc cggcctgaac gatctgctcc agcaccccga
     4201 gcacctgctg acgctacagg gcatcgccga cctgctgggc atcatcaagc cgctgctcaa
     4261 cgccgggctg gacatcgccg gtgcggtcat ccacgccatg ctcgaactga tgggcatcgt
     4321 gctgaacgcg ctgctcgacc tgttca
//
LOCUS       DASUYC010000473         3006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_557284,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000473 DASUYC010000000
VERSION     DASUYC010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557284"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..1473
                     /locus_tag="VF276_07800"
     CDS             130..1473
                     /locus_tag="VF276_07800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6606801.1"
                     /translation="MKICLVSPYDYPYPGGVTEHIKGLEKYLRLRGHQVTILAPSSSD
                     EDQFLAGNVRRVGDVVELPANGSRARISFSLGISRVVRDILTTEAFDVIHLHEPLMPM
                     VPLIVLRQSETCNVGTFHAFGDESLAYAAARPVLRGYFERLHGRIAVSAAARDYISQY
                     FPDDYTIIPNGIDVARFSAPLAPIARYRDGRPTVLFVGRFDEPRKGFRTLLQALPLVR
                     TVCPDVRVVVVGKGDAELFADLLPEEPDVVEFAGYVSAEELPRYYQSCDVYCAPSTGQ
                     ESFGIVLLEGMAAGVPVVASRCVGYAAVMTHGKEGFLVEPRSPEALAVALVRVLSDGQ
                     LRREMVAHGRETAARYDWPVIAARVEAFYEQARARFLAEGGPPHRFSLRRLRHLTPLS
                     GAPGWPLADELEQGLDRATARISDWLGVETDGSPRRPRRPGAGRRSDEDHAADSE"
     gene            1587..2207
                     /locus_tag="VF276_07805"
     CDS             1587..2207
                     /locus_tag="VF276_07805"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX6606802.1"
                     /translation="MLTSVFQDWTRAQMRRIATIFVNTPITPNMLTMFGLLINIGVAA
                     LIATGNLFWAGILIVFAGLFDLLDGALARITNRITPFGGFLDSSLDRYSEGVIYMGLL
                     WYFLQRGRTLEPMLIFAVIVGSMMVSYTRARATALNVDAEVGIMARSERIGALAIALI
                     LSPVLPDIVTWALWLVAIGAQVTTIQRILHVYNQAGKTPVREPAKR"
     gene            complement(2215..2394)
                     /locus_tag="VF276_07810"
     CDS             complement(2215..2394)
                     /locus_tag="VF276_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606803.1"
                     /translation="MLYLIVLGALILLIGVLWAVSGAPAAGWVLAATVVVGLALPVAR
                     ILLRRGGPDDKFPWE"
     gene            2713..2788
                     /locus_tag="VF276_07815"
     tRNA            2713..2788
                     /locus_tag="VF276_07815"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2746..2748,aa:Ala,seq:cgc)
BASE COUNT          476 a          972 c         1012 g          546 t
ORIGIN      
        1 ggggggccgc ttcatgaact tccgcgacca cgccgtcatc gccgcgccca acctctggcg
       61 cagcatgatt gtgtctccgg cctataaggt ccagcgggcg gtgcgccggg gcgtccgcag
      121 gcaggcgaca tgaaaatttg cctggtctcg ccgtatgatt atccctatcc cggcggcgtg
      181 accgaacaca tcaaggggct ggaaaaatac ctgcgcctgc gcggccacca ggtgacgatt
      241 ctcgcgccct cgtccagcga cgaggatcag tttctggccg gcaacgtgcg ccgggtgggg
      301 gatgtggtcg agttgccggc caacgggtcg cgggcgcgga tttccttctc gctgggcatc
      361 agccgggtcg tgcgcgacat cctgaccacc gaagccttcg acgtgatcca cctgcatgaa
      421 ccgttgatgc ccatggtgcc gctgatcgtg ctgcggcagt cggagacgtg caacgtcggc
      481 accttccacg cctttggcga cgagagcctg gcctacgccg ccgcccggcc cgtgctgcgc
      541 ggctatttcg agcggctgca cgggcggatc gccgtctcgg cggcggcgcg cgactacatc
      601 agccagtact tcccggacga ttacaccatc atccccaacg ggatcgatgt ggcgcgcttc
      661 agcgcgcccc tggcgcccat cgcccgctac cgcgatgggc ggcccacggt gctgttcgtg
      721 ggccgcttcg acgagccgcg gaagggattc cgcacgttat tgcaggccct gccgctggtg
      781 cgcacggtct gccccgatgt gcgcgtcgtc gtcgtcggca agggcgatgc cgaactgttc
      841 gccgatctgc tgcccgaaga gccggacgtg gtcgaatttg ccgggtatgt gagcgcggag
      901 gagttgccgc gctactacca gagctgcgat gtctattgcg cgccctcgac cggccaggag
      961 agcttcggga tcgtgctgct ggagggcatg gctgccgggg tgcccgtcgt ggcgagccgc
     1021 tgcgtgggct acgctgccgt gatgacgcac ggcaaagagg ggttcctggt cgaaccccgc
     1081 tccccggaag ccctggctgt ggccctggtg cgggtgctga gcgatgggca actgcgccgg
     1141 gagatggtgg cgcacgggcg cgagaccgcc gcgcgctacg actggcccgt gatcgcggcg
     1201 cgcgtggaag cgttttatga gcaggcgcgc gcgcgattcc tggccgaggg cggcccgccc
     1261 catcggttct cgttgcgccg gttgcgccat ctcacgcccc tcagcggcgc gccgggctgg
     1321 ccgctggccg acgagttgga acaaggtctg gatcgtgcta cagcacgtat tagcgactgg
     1381 ctcggcgtgg aaacggacgg atcgccccgg cgcccgcggc gccctggtgc gggccgccgg
     1441 tcggacgagg atcacgccgc cgattctgaa tagtggtggt ggcccgtgcc tgctgggcca
     1501 ccaacgacag gacgacgcgc gccggtacgc ggcgagggtc ctggcgcccc cggccctgaa
     1561 gggggctttg tgggaggaga ctgacgttgc tgacgagtgt ttttcaggat tggacccgtg
     1621 cgcagatgcg gcggatcgcc accatctttg tcaacacgcc gatcacgccc aacatgctga
     1681 cgatgttcgg gctgcttatc aatatcggcg tggccgcgct catcgccacc ggcaacctgt
     1741 tctgggccgg catcctgatc gtgttcgcgg ggctgttcga tctgttggac ggggcgctcg
     1801 cgcgcatcac caaccgcatc acgccgttcg gcggcttcct cgactcgtcg ctcgaccgct
     1861 attccgaagg cgtgatctat atggggctgc tgtggtactt cctgcagcgg ggccgcaccc
     1921 tggagccgat gctcatcttt gccgtgatcg tcgggtcgat gatggtcagc tacacgcggg
     1981 cgcgggcgac ggcgttgaac gtcgatgccg aggtggggat catggcgcgc tccgagcgca
     2041 tcggcgcgct ggcgattgcc ctgattctga gcccggtgct gcccgacatc gtgacctggg
     2101 cgctctggct ggtagccatc ggcgcgcaag tcaccaccat ccagcggatc ctgcacgtct
     2161 ataatcaggc ggggaagacg ccggtgcgcg aaccggcaaa gcgttaagcg ggcctcattc
     2221 ccaggggaac ttgtcgtcgg gcccaccgcg ccgcaagagg atgcgcgcca cgggcaaggc
     2281 cagccccacg accacggtgg ccgcgagcac ccacccggcc gccggggcgc cgctgactgc
     2341 ccagagcacc ccgatcagca ggataagtgc gccaagtacg atcagatata acacccagga
     2401 tcccctctct gctctcgtac gccccattat atcacaggcg tcacggcggc ggcttcttca
     2461 ttaccaagct gtaagggcgc gggcgccgcg cggcccgcgc gcaccctgtc tatctactgc
     2521 aatacgcccg cgccgggccg gttacgcgcc gccgcctcgc gttaccaggt gccgcatggc
     2581 cgcgccgccc gcaaagaatc gggctgatat gaactttctt cgcgttccgc gtgtctgcgc
     2641 ggtgcacctg ggaacagatg gccgtatgtg ccttgcaaat cgccgggact tctgctataa
     2701 taaggggcgc ctgggtctgt agctcagctg ggagagcgct acattcgcat tgtagaggtc
     2761 agcggttcga gcccgctcag atccaccagg aaaagaaact aatattaggc acgaaaaggc
     2821 cccgacggaa ggtttcgggg cctttttttt gcctttttgt aagctatgta gccaaagatg
     2881 tagccaaccg tggcgcgggc tgtttgctgc acaagagaaa ttgcagggcg ttcgctcaag
     2941 gggcgccgct gagcaaatcg tccatccggt ctgcggcctg ctgacgagtg ctgtcgaaca
     3001 catggc
//
LOCUS       DASUYC010000474         3190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1410685, whole genome shotgun sequence.
ACCESSION   DASUYC010000474 DASUYC010000000
VERSION     DASUYC010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410685"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..2954)
                     /gene="acnA"
                     /locus_tag="VF276_07820"
     CDS             complement(99..2954)
                     /gene="acnA"
                     /locus_tag="VF276_07820"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEX6606804.1"
                     /translation="MNSFGARSTLAVGGREYEIYRLAGLAHLGYDVARLPYALKICLE
                     NLLRTEDGRAVTADHIARLAAWNPHATPDTEIAFTPARVLLQDFTGVPAIVDLAAMRD
                     ALARLGGDPTRINPVQPVELVIDHSVQVDAYGSAAALLTNAEREFARNRERYAFLRWG
                     RQALQNFQVVPPGTGIVHQVNLEYLARVIFTTAADQTGGPDSLPQAYPDTVVGADSHT
                     TMINGLGVLGWGVGGIEAEAAMLGQPVSMLIPQVAGFRLEGALREGVTATDLVLRVTE
                     MLRNKGVVGKFVEFYGPGLPTLPIADRATIANMAPEYGATCGIFPIDAETLRYLRLTG
                     RSEDHVALVEAYSKEQGLFHTADSPEPLYSDTLELDLSTVEPSIAGPKRPQDRVALSG
                     ARASFETALPDMLHPAGPPAPQPHGRGGGGAGGAPTAGGIAQEIALLTAVKVQLAQGE
                     FALDHGAVVIAAITSCTNTSNPSVMVAAGLLARKAVERGLATPPWVKTSLAPGSRVVT
                     EYLQAAGLAPYLDALRFHTVGYGCTTCIGNAGPLPAPISRAVDEGKLVVCSVLSGNRN
                     FEGRINADVKANYLMSPPLVVAYALAGRMTIDLTTEPLGTGTDGQPVYLRDIWPSRQE
                     IAATMAAAIRSEMFRRSYAEVFAGDARWHGLDTPTGDRFAWDPDSTYVRHPPFFEGMT
                     REAPERVPEITGARVLAVLGDSVTTDHISPAGSIRAASPAGQYLLDHGVDPRDFNSYG
                     ARRGNHEVLVRGTFANPHLRNRLAPGTAGGFTTYLPTGEVTPIYDAAMRYQASGVPLI
                     VLTGKEYGTGSSRDWAAKGPALLGVRAVIAESFERIHRSNLIGMGILPLQFLAEESPA
                     LLGLSGHEVFDITGLAGAVASGFADGQQLKVRATRPDGGVLEFAVRVRIDTPQELRYY
                     QHGGILEYVLRQALAPVDAGDPRRTATVPDSEPVETC"
BASE COUNT          509 a         1042 c         1129 g          510 t
ORIGIN      
        1 tctgccctgg ccggccccgg cagtccgcgg catgtcatcg tcctttgccc ccactgatgc
       61 tcagcctgtc gcctgtgtag gtcggcagcg gcgggcgatc agcacgtttc gacgggttcg
      121 gaatcaggca ctgtcgcggt gcgccgggga tccccggcgt cgaccggggc cagcgcctgg
      181 cgcaggacat actccaggat gccgccgtgc tggtagtagc ggagttcctg tggcgtgtcg
      241 atgcgcaccc gcaccgcgaa ttccagcacg ccaccgtccg ggcgcgtcgc tcgcaccttg
      301 agttgctgac cgtccgcgaa cccgctcgcc acagcgccag ccaggccggt gatgtcgaag
      361 acttcgtgtc cgctcaggcc cagcagcgcc ggcgactctt cggccaggaa ctgcaacggc
      421 aggatgccca tgccgatcag gttcgagcgg tggatgcgct cgaagctctc ggcgatgacg
      481 gcgcgcaccc ccagcagggc cgggcctttg gccgcccagt cgcggctgga gccggtgccg
      541 tactccttgc cggtcaggac gatcagcggc acgccgctgg cctggtaacg catcgcggcg
      601 tcgtagatgg gcgtcacctc gccggtcggc agataggtgg tgaagccgcc cgcggtgccc
      661 ggtgcgagcc ggttgcgcag atgcgggttg gcgaaggtgc cgcgcaccag cacctcgtgg
      721 ttgccgcgcc gcgcgccgta gctgttgaag tcgcgcggat ccacgccgtg atccagcagg
      781 tactggccgg cgggcgaggc cgcgcggatg ctaccggcgg gcgagatgtg gtcggtggtc
      841 acgctgtcgc ccagcacggc gagcacccgc gcgccggtga tctccggcac gcgctccggc
      901 gcctcgcggg tcatgccctc aaagaatggc gggtggcgca cgtaggtcga gtccgggtcc
      961 caggcgaacc ggtcgccggt gggcgtgtcc agcccgtgcc agcgtgcgtc gcccgcaaac
     1021 acctcggcat agctacgccg gaacatctct gaccggatcg cggcggccat cgtcgcggca
     1081 atctcctgcc ggctgggcca gatgtcgcgc aggtagacgg gttggccgtc tgtgccggtg
     1141 cccagcggct cggtggtcag gtcaatggtc atgcgcccgg ccagcgcgta ggccaccacg
     1201 agcggcggcg acatcaggta gttggctttc acgtccgcat tgatccgccc ctcgaagttg
     1261 cggttgcccg acagcaccga gcagacgacc agcttgccct cgtcgacggc cctggaaatc
     1321 ggagcaggga ggggcccggc attgccgatg caggtggtgc agccatagcc caccgtgtgg
     1381 aagcgcaacg catcgaggta gggcgccagg cctgccgcct gcaaatactc ggtgacgacc
     1441 ctggagccgg gcgccagcga ggtcttaacc cagggcggcg tcgccagccc gcgctcgacg
     1501 gccttccggg ccagcagccc cgccgccacc atcaccgagg ggtttgaggt gttggtgcaa
     1561 ctggtgatcg ccgcgatcac cacggcgccg tggtccagcg cgaactcgcc ctgcgctagc
     1621 tggaccttga cggcggtgag cagcgcgatt tcctgggcga tgccgccggc agtgggggcg
     1681 ccgcccgcgc ctcctccgcc gcgcccatgg ggctgcggag cgggcgggcc ggccggatga
     1741 agcatgtcgg gcagggcggt ctcgaaggac gcgcgagcgc cggagagagc cacccggtcc
     1801 tgggggcgct tgggcccggc gatgctcggc tcgaccgtgc tcaggtccaa ctccagcgtg
     1861 tccgagtaca gcggctcggg cgagtcggcg gtgtggaaga ggccctgctc cttgctgtag
     1921 gcctcgacca gcgccacgtg gtcctccgag cgccccgtca ggcgcaggta gcgcagcgtc
     1981 tcggcgtcga tggggaagat gccgcacgtg gcgccatatt cgggcgccat gttggcgatt
     2041 gtcgcccggt cggcgatggg cagcgtgggc aagccggggc cgtagaactc cacgaatttg
     2101 ccgaccacgc ccttgttgcg cagcatctcg gtgacgcgca acaccaggtc ggtggcggtc
     2161 acgccttcgc gcagcgcacc ctctagccgg aagccggcga cctgggggat gagcatcgac
     2221 acgggctggc cgagcatggc cgcctcggct tcgatgccgc cgacgcccca gcccagcacg
     2281 cccagcccgt tgatcatcgt cgtgtgcgag tccgccccga cgaccgtgtc ggggtaggct
     2341 tgcggcaggg aatcggggcc gccggtctgg tcggcggcgg tggtgaagat gacgcgggcc
     2401 agatattcga ggttgacctg atggacaatg ccggtgccgg ggggaacgac ctggaagttc
     2461 tgcaaagcct gccggcccca gcgcaggaag gcgtagcgct cccgattacg cgcaaactcc
     2521 cgctcggcgt tagtgagcag ggccgcggcg gagccgtagg catccacctg caccgagtgg
     2581 tcgatgacca actcgaccgg ctgcacgggg ttgatgcggg tggggtcgcc gcctagccgg
     2641 gccagggcgt cgcgcatggc cgccaggtcc acgatagcgg gcacgccggt gaagtcctgc
     2701 aagagaacgc gcgcgggcgt gaaggcgatt tcggtatcag gggtggcgtg cgggttccag
     2761 gcggccaacc gggcgatatg atctgcggtg acggcccgac cgtcttcggt ccgcagtaga
     2821 ttctccaggc agattttaag ggcatagggt aaacgggcga catcgtatcc caggtgggcc
     2881 agccccgcca gccggtaaat ctcatactcg cggccaccca ccgccagggt ggaccgtgcc
     2941 ccaaagctat tcatggctgc tatctccaaa taggactgag ttatgcggcg ccgtccggcc
     3001 gcttcgacaa accgattata gatctgccca gactatttgt atagacaata gagctaatca
     3061 tattgattgg atttccttat caaacgtgct gcggatggcg tataataaag ccggtggaga
     3121 aggtaagtta cacgctgtcc ggacagcaaa cagccgggag caatgcgatg gacctgcata
     3181 aactgcgtat
//
LOCUS       DASUYC010000475         2325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1255247, whole genome shotgun sequence.
ACCESSION   DASUYC010000475 DASUYC010000000
VERSION     DASUYC010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1255247"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            432..2024
                     /locus_tag="VF276_07825"
     CDS             432..2024
                     /locus_tag="VF276_07825"
                     /inference="COORDINATES: protein motif:HMM:NF021576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HEX6606805.1"
                     /translation="MLRPRLLERLDAGLHRKLTLIAAPAGSGKTTLLTAWLAGSQRQV
                     AWLSLDAADGEPARFLTYLVAALRTVAAPVGEDLLPALQAPQPPPSEAILTTLLNEIA
                     AVPHRLVLVLDDYHLIDAHAIDEATTFLLEHLPPQLHLVIASREDPPLPLARLRARNQ
                     LTEVRAADLRFTPAEAAAFLQAMNLQLSADEVAALEERTEGWIAGLQLAALSLQGRRD
                     VPASIRAFAGDHRYILDYLGEEVLAHQPAAVRRFLLHTAILERLTGALCEAVTGQEGG
                     SARLEALERGNFFVVPLDERRQWYRYHHLFAEVLLAHLRAEQPDLLPTLHRRASAWYE
                     QHGLAAEAITPANARFFAAPLPVVVHIVCAGFYAILGAFQFADGFRRRWPGWHRLAGR
                     LLVGCGLLVGLSGLWMTLFYALPPSDGPLLYVFRLLFGAAMVASIILGFITVRRGDVR
                     GHRAWMARGYALGLGAGTQVVTLAVGEMIAGPPPENSRALLMGAAWAINLAVAEWAIR
                     RRPAHVTAARAHPASVVGSCLR"
BASE COUNT          360 a          785 c          764 g          416 t
ORIGIN      
        1 tactccgcga tctccgttcc cattgcgcgc ccggcgggta cctgtttgcc ggctgtgtag
       61 catacaccgg atctcatggc ctgggggaag gcgcccacga tggcgaacaa gagcgccgat
      121 gtgaagcaag ctccgcttgt acccgaggcc ggcgccggcg cgcgtactgg ggacccgttc
      181 gccggaattg cctacctgga gcgcccgacc acctgcgccg ggggcgggcc cgccggccgt
      241 ttactgccca tccgtaaaca cgcacggccg ggggtccgtc gcgcgctacc acgcagcagg
      301 gccccgctgt ggcgaggagg cgcggccgcc ctggctctaa gtggccgttt ggcggactgc
      361 cctatacttg atccatgtct ataccagttt taactactaa actctatatt ccccaaccgc
      421 caccaggggc ggtgctgcgt ccccgcctgc tggagcggct ggacgcgggt ttgcaccgca
      481 agctgacgct catcgcggct cccgccgggt ccggcaaaac cacgctgctg accgcctggc
      541 tcgccggcag tcagcgtcag gtggcctggt tgtcgctgga tgcggcggac ggcgagccgg
      601 cccgctttct gacctacctg gtcgccgctt tacgtaccgt ggccgccccg gtcggggaag
      661 acttgttgcc ggccctccag gcaccgcagc ccccgccaag cgaagccatc ctgactaccc
      721 tgctcaatga aatcgccgcc gtcccccacc gcctcgtgct cgtcctcgac gactaccacc
      781 tgatcgacgc ccacgccatt gacgaggcca cgaccttctt gcttgagcac ctgccaccac
      841 agttgcacct ggtcatcgcc agccgcgaag accccccgct cccgctggcg cggctccgcg
      901 cccgtaacca gctaacagag gtgcgcgccg ccgacctgcg ctttacccct gcggaagccg
      961 ccgcctttct ccaggcgatg aacctacagc tctcggcaga cgaggtggcg gccctggaag
     1021 agcgcaccga gggctggatc gccgggctgc aactggctgc gctctcgctg cagggccggc
     1081 gcgacgtgcc cgcctcgatc cgggcgttcg ccggcgacca tcgctacatc ctggactacc
     1141 tgggcgaaga ggtcctcgcg caccagccgg cggccgtgcg ccgcttctta ctccacactg
     1201 ccattctcga acgtttgact ggcgcgctgt gcgaggccgt caccgggcag gagggcggca
     1261 gcgcgcgtct ggaagccctg gaacgcggca acttttttgt cgtgccgctg gatgagcggc
     1321 gccagtggta tcgctatcac cacctctttg ccgaagtgct cctggcgcat ttacgggcgg
     1381 aacaacccga tctgctaccg acactgcacc ggcgggcgag cgcttggtac gagcagcacg
     1441 gcttggcggc ggaggcgatc acgccggcca acgcacggtt cttcgcggcg ccgctgcctg
     1501 tcgtggtgca tatcgtatgt gctggcttct atgccattct cggcgcgttc cagttcgcgg
     1561 acgggttccg gcggcgctgg ccgggctggc accgcctggc cgggcggctg ctggtcggct
     1621 gtgggctgct ggtcgggctc tccgggctgt ggatgacgtt gttctatgcc ctgccgccga
     1681 gcgacggccc gctattgtac gtgttccggc tcctgttcgg cgcggccatg gtcgcctcga
     1741 tcatcctggg cttcatcacc gtccgacggg gggacgtgcg cgggcaccgc gcctggatgg
     1801 cgcgcggcta cgcgctcggg ctgggcgccg gcacccaggt ggtgaccctg gcggttgggg
     1861 agatgatcgc cggcccgccg cctgagaaca gccgagcgct tttgatgggc gcggcgtggg
     1921 cgatcaacct cgccgtggcc gaatgggcta tccgcaggcg accggcccac gtgactgctg
     1981 cgcgcgctca cccggcatca gttgtcggct cctgtctgcg ctgaggcata gatcaccagg
     2041 tcaatcacca agtttagtga gccggcatca ctatattggt ttgtatgctc ttgtggaagg
     2101 caagaagccg agcagcgacg aggggcttaa gccagatgag gaatacacgg gaggcccaga
     2161 atgggtataa caaatatgat cagagaaggc tcgggcgaga ctggacacta cgagatacga
     2221 atcaaggggc accttgaagg caggtgggcc gtctggtttg agggcctgac cctgacgttg
     2281 gaagagaacg gtgatacgct gttaaccggc ccggtgatcg atcaa
//
LOCUS       DASUYC010000476         5776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_743243,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000476 DASUYC010000000
VERSION     DASUYC010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5776
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743243"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..1334)
                     /locus_tag="VF276_07830"
     CDS             complement(120..1334)
                     /locus_tag="VF276_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002620961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX6606806.1"
                     /translation="MTQVAQPNLASQQFKADPHPFYTYLRHEAPVYRAALPDRRVAWL
                     LARYDDTIMLLKDERFVKDPANAQPPGEPPRTQWVPGFARPLARNMLDLDAPDHARLR
                     LLVGKVFTRGLVEQLRGRVETLCDGFLAAAQRRGSLDLIRDYAVPLPTTIIAEVLGVP
                     VEDRTRFHRWSARIIATSSGMDQLGALPSLWLFVRYLQALFQQRRAQPRADLISQLIQ
                     VQEAGERLSDDEMLSMVFILLIAGHETTVNLIGTGTLALLDHPAQAERLRRDPAIIGP
                     AVEELVRYASPVELATERYAREDITTHDCTIPRGEMVLGVIGSANRDEQHFVNGQDLD
                     LTREPNKHLAFGQGAHYCLGAPLARLEGQIAINMLLQRFPKLRLAVPRESLPWRRGIF
                     LRGLSQLPLLVD"
     gene            complement(1536..2168)
                     /locus_tag="VF276_07835"
     CDS             complement(1536..2168)
                     /locus_tag="VF276_07835"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX6606807.1"
                     /translation="MADKSQSPAQEEQIAARRKQILGAAIKVFAAKGAHRATIREIAR
                     AAGLADGTIYNYFENKNALLLGILDRLNETEQREAHFAQAEALGLDQFLELYLKQRLD
                     RLTEDGLDVLRVLFSELLVNAELRETYYQAVMKPTTEAGEASFRQWHAQGAPAFSDPA
                     MATRVVAGAVIGLIIMRLLGDEYLQAHWSELPDALAAVIRRGIMPNGATC"
     gene            complement(2344..>5776)
                     /locus_tag="VF276_07840"
     CDS             complement(2344..>5776)
                     /locus_tag="VF276_07840"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6606808.1"
                     /translation="ARLRHGQGGFVNEYRAVTMLFAAFSGFDYDHDPDVVAKLQDYFG
                     AVTRIVARYDGYLNKIDIGDKGSTYLVLFGAPVAHENDPERALRCAAELQALPGVPTH
                     MGLNTGLVYCGNVGAAARQEYTVIGGAVNLATRLMQVAGSGQILLTDFTRRLVPGSIH
                     CEELPPIGVKGLAEPVAISALVGLDGRGPLRLQGQTYTLPMVGREAEKALAAARLDDV
                     LGGAGQILGITAEAGMGKSRLNSEIWRLAGQAGLQCYAGECQSYGTNVPYLVWQDLLR
                     GFYGVDPTASDDAQIRTLTAAVEQIDPASVPRLPLLGVALNMPIPETEITRSLDAKLR
                     KAALEALLIHAIRARAQDRPLLLVLEDCQWIDPLSLDLLEAVARNIADVPVLIVLVYR
                     TLPQATTEGDRITALPYFTELRLAEFTRAEAERLIRMKVARLFGAEDNVPLALIRRIT
                     ERAQGNPFYIDEMINLIHDQGIDPTDLEALQHLDLPASLRSLIISRIDQLPEGEKTTL
                     KVASVIGRVFPAAWIWGCYPQLGPHEQVLRYLSTLSQMDLTHLDQPEPELIYLFKHIV
                     TQEVAYQSLAGATRAKLHGQIGAFLEQTYGDDIQRWITLLAHHYSLSGDVAKQREYFL
                     RAAEAAQAAYANDAAIEYYRRLLPLLPRPEQTAVRLKIGGILQLIGHWSEAEELYRRA
                     LQKFETARDPQTRARLQQALGDLLGLKGSYGEALLWLQRAHKTCGEIEDRQGMARAIG
                     GIGHIYWRQGNYADALACAEQQLAGATEIGDQEQAGQALGNFGRIYWEQGEYGRALAS
                     YSRQLQIATALGDRPSMILALGSMGNVYWQQGEYAAALACFEQSLLRAVEIDDRRGIS
                     ITVGQMGTVYWRQGNYAQALACFGRQLTIATEMGERRSISEALGNMGIVYGEYGDYRR
                     SLVCLQQQLAMAGKTGDRKYMSIAVANMARAYTAQGRYAEADRLYSQAVELADLLNNP
                     YLRCEYLHGQAGLYALLGRPADAQSLNDVALRQAVAVQRRDIQFQAELLAIRLGVTLR
                     QISPATALPALQHMESLWTEPDQQAALRYEGWRIDSSRADLRESAALAYHTLYTAQPQ
                     IEYRRRYQDLTGRRLPPPPPLPDLPPAAARESLDPARLLDRVDATLAQMAAAQAS"
BASE COUNT          888 a         1829 c         2040 g         1019 t
ORIGIN      
        1 aaaggtagtc ttcccctcgg gaatcgtcat tctgagcgga gcgaagaatc tcgtctcgca
       61 cggggcagaa cgagactctt cgctgcgctc ggagtgacaa ggccctctgg aagtggtact
      121 cagtcaacca gcagcggcaa ctgactgagg ccgcgcagga agatgccccg ccgccagggg
      181 agcgactccc gcggcaccgc caggcgcagc ttggggaagc gttgcagcaa catattgatg
      241 gcgatctggc cctccagccg ggccagcggc gcgcccaggc agtagtgggc gccctggccg
      301 aaggcgagat gcttattggg ttcgcgggtg aggtccaggt cctggccgtt cacgaagtgc
      361 tgttcgtcgc ggttcgccga gccgatcacg cccagcacca tctccccgcg cggaatggtg
      421 cagtcatggg ttgtgatgtc ctcccgcgcg tagcgctcgg ttgccaactc gaccgggctg
      481 gcgtagcgca ccagttcctc cacggcaggc ccgatgatcg ccgggtcccg gcgcaatcgc
      541 tcggcctggg ccgggtgatc cagcagcgcc agcgtgcccg tgccaatcag attgacggtc
      601 gtttcgtgcc cggcgatgag caggatgaag accatcgaga gcatctcgtc gtcgctcagg
      661 cgctctccgg cttcctggac ctggatgagt tggctgatga gatcggctcg cggctgggcg
      721 cgccgctgct gaaatagcgc ctggaggtag cgaacaaaga gccacaggct cggcaacgcc
      781 cccagttggt ccattcccga tgaagtcgcg atgatccgcg cggaccagcg gtgaaagcgc
      841 gtccgatcct ccaccggcac gcccagcacc tcggcaatga tcgtggtggg caggggcacg
      901 gcgtaatcgc ggatcaagtc cagcgacccg cgccgttggg ccgcggccag gaatccgtcg
      961 catagggtct ccacccgccc gcgcagttgc tccaccagac cgcgcgtgaa caccttgccc
     1021 accagcaacc gtaagcgcgc gtggtccggg gcgtcgaggt ccagcatgtt acgcgccagc
     1081 ggtctggcaa agccgggcac ccactgggtc cggggcggct ctccgggcgg ttgggcattg
     1141 gcgggatctt tgacaaagcg ctcgtccttg agcagcatga tcgtatcgtc atagcgggcc
     1201 agcagccagg ccacgcgccg gtcgggcagg gcggcccggt acaccggtgc ttcgtggcgc
     1261 aggtacgtat agaagggatg cgggtcggcc ttaaactgct gactcgccag attcggctgg
     1321 gctacctggg tcattgtcat tatcgtcacc cccattaggg cgatgtgcta gatcaagtaa
     1381 attcaaaaag gctaatacga gttattgtgc agctaacgga gcggcccgtc cgggttctaa
     1441 gccctgctgg atgcccctga tctgctgttt agcccgactt cgcacgagct tttacgcagg
     1501 ttgtcctggc ttcagcctgt taaaactaac ttgatctagc acgtcgcccc attaggcatg
     1561 atcccccggc ggatcaccgc ggccagtgcg tccggaagtt cgctccagtg cgcttgcaga
     1621 tattcgtcgc cgagcaagcg catgataatc aggccgatca ccgcgcccgc taccactctg
     1681 gttgccatgg ccgggtcgga gaaggccggc gccccctgcg cgtgccactg gcggaaactg
     1741 gcttcgccgg cctcggtcgt gggcttcatc actgcctggt aatacgtttc ccgcaactcg
     1801 gcattcacga gcagttccga gaacaagacc cgcagaacgt cgaggccgtc ctcggtcagc
     1861 cggtccaacc gctgcttgag atacagttcc agaaactggt ccaggcccag ggcctcggcc
     1921 tgggcgaagt gggcttcccg ctgctcggtc tcgttcaggc gatcgaggat gccgagcagc
     1981 aaggcgttct tattctcgaa gtagttgtag atcgtgccgt ccgctaatcc ggcggcgcgc
     2041 gcgatctccc ggatagtggc gcggtgggcg cctttcgcgg cgaatacctt gattgccgcg
     2101 ccaagaatct gcttgcgtcg tgccgcgatc tgttcttcct gtgcaggact ctgcgattta
     2161 tcggccatag ctctcctgag tcatagattc cacatctggt gaatgaacat tcattcacca
     2221 gacagtgtac tcctctttgc cgaggaaagc aataggttgg gcggccgttt gagggtggag
     2281 attgtctagt agacagggtg tgcgcagctt aggaaaggcg cgagacggcg cgggcgagtg
     2341 cgcttagctg gcctgggcgg cggccatctg ggccagggtg gcgtccacgc ggtcgagcag
     2401 ccgggccggg tcgagggact cgcgggcggc ggcgggcggg aggtcgggca gcggcggcgg
     2461 gggtggcagg cgccggcccg tcaggtcctg gtagcggcgg cggtactcga tctggggctg
     2521 ggccgtgtag agggtgtggt aggcgagggc agcgctctcg cgcaggtcgg cgcggctgga
     2581 gtcgatccgc cagccttcgt agcgcagggc ggcctgctga tccggctccg tccagaggct
     2641 ttccatgtgc tggagggccg gcagggcggt ggccgggctg atctggcgca gggtgacgcc
     2701 caggcggatc gccaggagtt cggcctggaa ctggatgtcg cggcgctgca cggcgacggc
     2761 ctggcgcagg gccacgtcgt tcagcgactg ggcgtcggcg gggcggccca ggagggcgta
     2821 gagcccggcc tggccgtgca ggtattcgca gcgcaggtag gggttgttga gcaaatcggc
     2881 cagttcgacg gcctggctgt aaagccggtc ggcctcggcg tagcggccct gcgcggtgta
     2941 ggcgcgggcc atgttggcga cggcaatgct catgtacttg cggtcgcccg tcttgccggc
     3001 catggcgagt tgctgttgca ggcagaccag gctgcggcga tagtcgccgt actcgccgta
     3061 gacgatgccc atgttaccca gcgcctcgct gatgctgcgc cgctcgccca tctcggtggc
     3121 gatggtgagt tgccgcccga agcaagccag cgcctgggca tagttgccct gccgccagta
     3181 gacggtgccc atctggccca cggtgatgct gatgccgcgc cggtcgtcga tctccacggc
     3241 gcgcaacagc gactgctcga aacaggccag cgccgccgcg tactcgccct gctgccagta
     3301 aacgttgccc atgctgccga gcgccaggat catgctgggc cggtcgccga gcgcggtggc
     3361 gatctgcaac tggcggctgt aactggcgag cgcccgcccg tactcgccct gttcccagta
     3421 gatgcgcccg aagttgccga gcgcctgccc ggcctgctcc tggtcgccga tctccgtggc
     3481 gccggcgagt tgctgctcgg cgcaggccag cgcgtcggcg taattgccct ggcgccagta
     3541 gatgtggccg atgccgccga tggcccgcgc catgccctgg cgatcttcga tctcgccgca
     3601 ggtcttatgg gcccgttgca gccagagcag cgcctcgccg tacgagcctt tgaggcccag
     3661 gagatcgccc agggcctgct gcaagcgggc acgggtctgg gggtcgcggg cggtctcgaa
     3721 tttttgcaag gcgcggcgat agagttcttc agcctcgctc cagtggccga tgagttgcag
     3781 gatgccgccg atcttgagcc gcacggcggt ctgctcgggg cgcggcagca gcggcagcag
     3841 gcggcggtaa tactcgatgg ccgcgtcgtt ggcgtaggcg gcctgggcgg cttcggcggc
     3901 gcgcaggaag tactcgcgct gcttcgccac gtcgccgctg aggctgtagt ggtgggccag
     3961 cagcgtgatc cagcgctgga tgtcgtcgcc gtaggtctgt tcgaggaagg cgccgatctg
     4021 gccgtgcagc ttggcgcggg tggccccagc caggctctga taggccacct cctgcgtgac
     4081 gatgtgcttg aacaggtaga tcagttcggg ctcgggctgg tcgaggtggg tcaggtccat
     4141 ctggctgagc gtgctgaggt agcgcagcac ctgctcgtgc ggccccagtt gcgggtagca
     4201 gccccagatc caggcggcag ggaacacccg cccgatgacg ctggcgactt tgagcgtggt
     4261 cttctcgccc tccggcaact gatcgatgcg gctgataatc aggctccgca ggctggcggg
     4321 caggtccagg tgctgcaacg cctccaggtc ggtcgggtcg atgccctgat cgtggatcag
     4381 gttgatcatc tcgtcgatgt agaaggggtt gccctgggcg cgctcggtga tgcgccggat
     4441 cagcgccagg ggcacgttgt cctcggcgcc gaacaggcgg gccaccttca tgcggatcag
     4501 gcgctcggcc tcggcgcggg tgaactcggc caggcgcaat tcggtgaaat agggcagcgc
     4561 ggtgatccgg tcgccttccg tggtggcctg cggcagcgtg cgatagacca gcacgatcag
     4621 cacgggcaca tcggcgatgt tgcgggccac ggcttcgagc aggtcgaggg acagtgggtc
     4681 gatccactgg caatcttcga gcaccagcag cagcgggcgg tcctgggcgc gggcgcggat
     4741 ggcgtggatc agcagggctt cgagcgccgc cttgcgcagc ttcgcgtcca gcgatcgggt
     4801 gatctcggtc tccgggatgg gcatgttgag cgccacgccg agcaagggca ggcgcggcac
     4861 ggacgccggg tcgatctgct cgacggcggc ggtgagcgtg cggatctgcg cgtcgtcgct
     4921 cgccgtgggg tccacgccgt agaagccgcg caagagatcc tgccagacca ggtaggggac
     4981 gttggtgccg taggactggc attcgcccgc gtagcattgc aggcccgcct gcccggccag
     5041 ccgccagatc tcgctgttca gccgcgactt gcccatgccc gcctcggcgg tgatgcccag
     5101 gatctgcccc gcgccgccca gcacgtcgtc cagccgcgcg gcggccagcg ccttctcggc
     5161 ctcgcgcccc accatcggca gcgtgtaggt ctggccctgc aagcgcagcg gcccgcgccc
     5221 gtcgaggccg accagcgcgc tgatcgccac cggctcggcc agccccttga cgcctatggg
     5281 cggcaattcc tcgcaatgga tggagccggg caccaggcgg cgggtgaagt cggtcagcag
     5341 gatctggccg ctgcccgcga cctgcatgag ccgcgtggcg aggttgaccg cgccgccgat
     5401 gaccgtgtac tcctggcggg ccgccgcgcc cacgttgccg caatagacga ggccggtgtt
     5461 gaggcccata tgggtcggca cgccgggcag ggcctgtagc tccgctgcgc agcgcagcgc
     5521 gcgctcgggg tcgttctcat gggccaccgg cgcaccgaac agcaccaggt aggtgctgcc
     5581 cttgtcgccg atatcgatct tgttcaggta gccgtcgtag cgggccacga tgcgcgtgac
     5641 ggcgccgaaa tagtcctgca actttgccac cacgtccggg tcgtggtcgt agtcgaagcc
     5701 gctgaacgcg gcgaagagca tggtgacggc gcggtactcg ttgacgaagc cgccctggcc
     5761 gtggcgcagt cgcgcg
//
LOCUS       DASUYC010000477         4120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_826970,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000477 DASUYC010000000
VERSION     DASUYC010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826970"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(800..1246)
                     /locus_tag="VF276_07845"
     CDS             complement(800..1246)
                     /locus_tag="VF276_07845"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX6606809.1"
                     /translation="MSVEENKAMLSRIYDECFNAHDLSAMDKYFAPDYIEHSPEVPQP
                     LDLQGFKNLFGMYLSAFPDARFTIEAVMGEGDMLAWRDTFTGTHQGELMGIPPTGKRI
                     SVTGVHYGRARDGMAVEHWAGVDTLGMLQQLGIVPMMGAAPAGAGT"
     gene            complement(1452..1649)
                     /locus_tag="VF276_07850"
     CDS             complement(1452..1649)
                     /locus_tag="VF276_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606810.1"
                     /translation="MKRRIQMQSRPLTLAEVCEAITNGRVPSEIEDGYYKVSRRALRQ
                     LQRPVDTRVTLSKERPADLAG"
     gene            2091..>4120
                     /locus_tag="VF276_07855"
     CDS             2091..>4120
                     /locus_tag="VF276_07855"
                     /inference="COORDINATES: protein motif:HMM:NF042648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="HEX6606811.1"
                     /translation="MGGTFLFIVTTVLLALGPLGAAPGAPAAHAAGGLGRPTSNRITA
                     ENALPGVDDLADLGNFDNARLAAYAGATSVNAGESLNLYVKATGTSLTWRLYRLGYYQ
                     NHGARLITTSSSSVPIGAQPVCTRNSSTGLVRCPWAATFTLNTDPGWISGVFLLRIDS
                     NNGYRTWVYFVVRNDSYAADIVVEQAAMTNQAYNLYGGESLYESHNNEGRQRAYQVSF
                     DRPYEASNGTGEFFHWDIDMVRWLEASGYDVTYITDLDTATNPNILLSHRIFLEMGHP
                     EYWTWGERDNVEAALAAGVNMVFATANETYWNIRLEASALGPNRIITCYKDTALDPTH
                     TAPYTTGRFHDPPLNRPENALIGLYFFDDNWYRTEGYNAPWVASAPANSWYFDCTGLQ
                     QGASVNNIVGYEYDTVYDNGYSPPGLEVVSTSPIINYRGQQTPANTIFYTAPSGARVF
                     AAGSLRWSWGLIDHSYANNPFQPDLLSNDADHRIEQLTANVIDSFAGYWDGQPRTCGG
                     QGFYDIGIRPTRTAMPQPPTATGTPPTATRTSTPAGTRTSTRTPTATPARTATVTGTP
                     PTLPPTVPAPSTNTPVPATSTPTPVPGGQPIAGAISSTDPTQAGRIVRDWVPSTCATV
                     KTVPGNGDMLAHHYDAYTFANTSGSAQCVTVKLDSACTGTNDVFSMAYLGSFT"
BASE COUNT          766 a         1363 c         1289 g          702 t
ORIGIN      
        1 cgggccgatg gcgagcttgg cgtcggggaa gattttcagc accgccccgg ccatgagatg
       61 cgcggcggag tggcgcatcc gcgccaggta ggcgccctcg ctctcgttgt cgtccaccgc
      121 atagggatgg ggcccgtaat cgatctcgtt ctctttatca acacccatgt ggctcataca
      181 acactccttt agccctagcc gtcagccgtc agccgtcagg agacgagatg acggccaaca
      241 tccgatcagc aatccctttt ataaggtcaa aaatggtcaa tacggcgaat tgaatgctga
      301 tagctgacgg ctgacggctg acggctgacg gctgatagct aatagctaca aaaaacctct
      361 cgcccgaaca acgggacgag aggcgagact cacgtggttc cacccgattt cacgccgccc
      421 gaacaggaag cagcgtcttc gtggggccgg taacggggcc atccggcgcg ggctaccggc
      481 ggcgcggggc cgcgttcgtc cggctgctcc cagggggttt tcgcgaaggc gccacaagcc
      541 cggcttgcag ccggggccgg gcctctctgc tgcgcgcgct ccgttactcg tcctgatcaa
      601 agcgtctggc tatgcgattc ccttgcatta tatgcgcggg tgggaggcgt gtcaaggata
      661 agtggtcagt ggtcggtggt cagtggtcag tacgatccgt ggttaagggg gtcggggcta
      721 gaggtgccgg cgggggcgcg cggcgggtcc tggtgcgccg ggcggcgcac caggatcggg
      781 aagcgcgcac cggaacgaat caggtgcccg ccccggcggg ggccgcgccc atcatcggca
      841 cgatgcccag ttgctgcagc atgccgagcg tatcgacgcc ggcccagtgc tcgaccgcca
      901 tgccgtcgcg ggcgcgcccg tagtggacgc ccgtgaccga aatacgcttc ccggtgggcg
      961 gaatgcccat cagttcgccc tggtgggtgc cggtgaaggt atcgcgccag gccagcatgt
     1021 ccccctcgcc catgaccgcc tcgatggtaa agcgggcgtc ggggaacgcg ctcaagtaca
     1081 taccgaacag gttcttgaag ccctgcagat ccagcggctg cgggacttcg ggcgaatgtt
     1141 cgatgtagtc cggcgcgaaa tacttgtcca tggcgctcag atcgtgagcg ttgaagcatt
     1201 cgtcgtagat ccggctcagc atagccttgt tttcttcaac agacatggga ttaacctcct
     1261 taatgtatcc tctccagtat cgctcaccgg ttgcgaagct cgaccagccc ggttgtttca
     1321 aggatagggc atcgggatgc tcaattccca gcccaaagga tgctcacttc ttgttgagga
     1381 gtcacggggc aaataaatag cctcgcgccc aggcgggcgg aggcgggtgc gttgcgacgc
     1441 ggatggggtg gttagcctgc cagatccgcc gggcgctcct tgctgagcgt gacgcgcgtg
     1501 tccaccgggc gctggagttg gcgcagagcc cgccggctca ctttgtaata accatcctcg
     1561 atctcggacg ggacacgccc gttggtgata gcttcacaca cttcggccag ggttaacggg
     1621 cgagactgca tctggatacg acgcttcatt ttcgtcactc ctaccattga cctcactgcc
     1681 gggccaatcg ctgttcggtc tccatggggc gggttgcggc accgctcccg gtcgcttgct
     1741 accgtgtgca ctcggtgatg ggatgggtaa gagcaggaag cgtgccaatt gggtagggct
     1801 gacataagga gcgtttgcgc ccaccggcgg aggaggtgtc ctcgccggcc cgtcaggggc
     1861 gcacacctga ctcgtccgga cagtgagtcc ttagtaaata gaaggagctt gcggaacgaa
     1921 aagttacggt atagaaagaa ttgattactg tcctggaggg gtcaagttat gatataatcg
     1981 agaggtatca ctccccgcta gatcgcgttc ccgctgctct gatattccgc acgccaccgc
     2041 agaaagggct ttatatggcc gcgcagccgc gccgaaccgc cggacggttc atgggtggaa
     2101 ccttcctgtt tattgtaacg actgtgctac tcgcgctcgg cccgcttggg gcggcgccgg
     2161 gcgcacccgc ggcccatgcc gccggggggt taggacggcc caccagcaac cgcatcaccg
     2221 cggagaacgc cctgcccggc gtggatgatc tggccgacct gggcaacttc gacaacgcgc
     2281 gcctcgcagc ctacgccggg gccaccagtg tcaacgcggg ggagtcgctc aacctgtacg
     2341 tgaaggccac cggcacctcg ctgacctggc ggctgtaccg gctgggctac taccagaacc
     2401 acggcgcgcg cctgatcacc acgagcagca gcagcgtgcc catcggcgcg caaccggttt
     2461 gcacccgcaa cagcagcacg ggcctggtgc gctgcccctg ggcggccacc tttaccctga
     2521 ataccgatcc cggctggatc agcggcgtct tcttgctgcg catcgactcg aataacggct
     2581 accgcacctg ggtctatttc gtcgtgcgca acgacagcta tgcggcggac atcgtcgtcg
     2641 agcaggcggc catgaccaac caggcctaca acctctacgg cggcgagagc ctctatgaat
     2701 cgcataacaa cgagggccgg cagcgcgcct atcaggtgtc gtttgaccgg ccctacgagg
     2761 ccagcaacgg caccggcgag ttcttccact gggacatcga catggtgcgc tggttggagg
     2821 cctcgggcta cgacgtgacc tatatcaccg acctcgacac ggcgacgaac cccaacatct
     2881 tgctgagcca tcgcatcttc ctggagatgg ggcacccgga gtactggacc tggggcgagc
     2941 gcgacaacgt cgaagccgcc ctggcagccg gggtcaacat ggtgttcgcc acagcgaacg
     3001 agacctactg gaatatccgc ctggaggcgt cggcgctggg gccgaaccgc atcatcacct
     3061 gctacaagga taccgcgctg gaccccacgc ataccgcgcc ctataccaca ggccgcttcc
     3121 acgacccgcc gctaaaccgc ccggagaacg cgctgatcgg gctgtatttc tttgacgata
     3181 actggtaccg cacggaaggc tataacgcgc cgtgggtggc gagcgccccg gccaatagct
     3241 ggtatttcga ctgcaccggg ctgcaacagg gcgccagtgt gaacaatatc gtgggctatg
     3301 agtacgacac ggtctacgac aacggctact cgccgccggg gctggaggtg gtgtccacca
     3361 gcccgattat caactatcgc ggccagcaga ccccggccaa cacgatcttc tacaccgcgc
     3421 cgagcggggc gcgcgtcttc gccgcgggca gcctgcgctg gtcctggggg ctgatcgacc
     3481 actcatatgc caacaacccg ttccagcccg acctgctctc gaacgacgcc gaccaccgca
     3541 tcgaacaact cacggccaac gtgatcgaca gcttcgccgg ctactgggac ggccagccgc
     3601 gcacgtgcgg gggccagggc ttttacgaca tcgggatccg cccgacgcgc accgccatgc
     3661 cgcagccgcc gaccgccacc ggcaccccgc ccacggccac gcggacatcg accccggcgg
     3721 gcacgcgcac atcgacccgc acgcccacgg ccacgccggc ccgcaccgcg acggtgacgg
     3781 gcaccccgcc cacgctcccg ccgaccgtgc cggcgccatc caccaacaca ccggtcccgg
     3841 ccaccagcac gccgactccg gtgcctggcg ggcagcccat cgccggcgcg atcagcagca
     3901 cggaccccac ccaggccggg cgcatcgtgc gcgactgggt gccgagcact tgtgccaccg
     3961 tcaagaccgt gcccggcaac ggcgatatgc tggcccacca ctatgacgcc tacaccttcg
     4021 ccaacaccag cgggagcgcc cagtgtgtca ccgtgaagct ggattccgcc tgcacgggta
     4081 cgaacgatgt gttcagcatg gcgtatctag gcagcttcac
//
LOCUS       DASUYC010000478         2380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1624259, whole genome shotgun sequence.
ACCESSION   DASUYC010000478 DASUYC010000000
VERSION     DASUYC010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1624259"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="VF276_07860"
     CDS             <1..626
                     /locus_tag="VF276_07860"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX6606812.1"
                     /translation="AFGLGKATGIELPGEIDAVAPDRAYKLKLNPNDFWSLGDTLFSA
                     IGQGFDLMTPLQLNNVTATVANGGTLYQPQIVYQVLNSDGDPKPIRDFQPKVISHVPV
                     NAEHLATIREGMRLAVSDIHKGTAYKTELKGVQIAGKTGTAEIGEVIDDKGHRRAHAW
                     FTAFAPYENPEIAVTVLIEAGDESLEGSTFAVPVARNIFKAYFHVDE"
     gene            636..1475
                     /locus_tag="VF276_07865"
     CDS             636..1475
                     /locus_tag="VF276_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015263426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HEX6606813.1"
                     /translation="MPSADPVIIKGVRDGLLILLDDDLGWPDLLAEVDARLSARRAFF
                     SGATVTANLGDRELSEAQFGALRSLFERFDMLLDVIVSTSEETRAVAGVNGVRNRAPA
                     FGRGQANTGVPEAGEPPPAIRPRRRRPPAPPDAAALPPAPDDGEPDFTALAGEDTTPV
                     AEATWAAGEPGALFVRRTLRSGMRILHDGDVCILGDVNAGAEIVAGGDVVVWGSLRGM
                     IHAGAGGDQAAVVCALLLAPTQLRIADLVSRGSEPSGPAAPEMARILEGRILVEPWSA
                     RRK"
     gene            1794..>2380
                     /gene="minD"
                     /locus_tag="VF276_07870"
     CDS             1794..>2380
                     /gene="minD"
                     /locus_tag="VF276_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007936206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HEX6606814.1"
                     /translation="MGRVITITSGKGGVGKTTSTANLGTALALLGKKVAVIDADIGLR
                     NLDVVMGLEKRIVYDIVDVVEGRCRIRQALIKDKRFPELYLLPAAQTRDKSAVNPAQM
                     RLLCDHLKTEFDFVLIDCPAGIEQGFKNAIAGADMVVIVTTPEVSAVRDADRVIGFLE
                     TAGITDIRLIVNRIRPAMVKGGDMMSVDDVLDILGA"
BASE COUNT          414 a          841 c          789 g          336 t
ORIGIN      
        1 gtgcctttgg cctcggcaag gcgaccggga tcgaactgcc cggcgagatc gacgccgtgg
       61 ccccggatcg ggcctataag ctcaagctga acccgaacga cttctggtcg ctgggcgaca
      121 cgctgttcag cgccatcggc cagggcttcg acctgatgac gccgctgcaa ctcaacaacg
      181 tcacggccac ggtcgccaac ggcggcacgc tctaccagcc gcagatcgtc taccaggtgc
      241 tgaacagcga cggcgacccc aagccgatcc gcgacttcca gcccaaagtg atcagccacg
      301 tgccggtgaa cgccgagcac ctggcgacga tccgcgaggg catgcgcctg gccgtctccg
      361 acatccacaa gggcacggcc tacaagaccg aactcaaggg cgtgcagatc gccggcaaga
      421 ccggcaccgc cgaaatcggc gaagtgatcg acgacaaagg ccaccgccgg gcgcacgcct
      481 ggttcaccgc cttcgcgccc tacgagaacc cggagatcgc cgtgacggtg ctgatcgagg
      541 ccggcgacga gagcctggag ggctcgacct tcgccgtgcc ggtggcgcgc aatatcttca
      601 aagcctattt ccacgtggac gagtagatcg cgcccatgcc gagtgcagat cccgtcatca
      661 tcaaaggcgt ccgcgacggc ctgctgattt tgctggacga cgacctcggc tggcccgatc
      721 tgctggccga ggtggacgcc cggctgagcg cgcgccgcgc cttcttcagc ggcgcgaccg
      781 tcacggccaa cctgggggat cgcgaactga gcgaagccca gttcggcgcg ctacgcagcc
      841 tcttcgagcg cttcgacatg ctgctcgacg tgatcgtctc cacgtctgag gaaacccgcg
      901 ccgtggccgg ggtcaacggg gtgcgcaacc gggcgcccgc gttcggacgc ggccaggcga
      961 acaccggcgt gcccgaagcc ggggagccgc cgcccgcgat ccgcccccgc cgccgccggc
     1021 ctcccgcgcc acctgatgca gccgccctgc cgcccgcgcc cgacgacggc gaacccgatt
     1081 tcaccgccct ggccggggaa gacaccacgc ccgtcgccga agcgacctgg gccgccgggg
     1141 agccgggcgc gctgttcgtg cggcgcacgc tgcgctcggg gatgcgtatc ctccacgacg
     1201 gcgacgtgtg catcctgggc gatgtgaacg ccggggctga gatcgtggcc gggggcgacg
     1261 tagtggtatg gggcagcctg cgcggcatga tccacgcggg cgcgggcggc gaccaggccg
     1321 ccgtggtctg cgccctgctg ctcgcgccca cccagttgcg catcgccgac ctggtcagcc
     1381 ggggcagcga accgagcggc ccggcggccc ccgaaatggc gcggattctg gagggccgga
     1441 tcctggtcga accctggagc gcccgccgca aataggcgcg gggcgcttcc agtctgttca
     1501 gaatcccttg cattctcggg cgatctggtg taaagtgaaa cgaacattca ggccacaggt
     1561 ctccgtgacg tagccgcgtg gcactgctgg gcctcccccc aatgcagatc ggagtgtagc
     1621 ggatgacgac ctacggacgc gaccggaacg gcaatccggt ctccctctcg ccggaaacgg
     1681 tgcctgcggg cgcccccacc aacggacatc acccgtaccc ggtgccgggt accaacggcc
     1741 acagccccgc gcccggcgcg gcgaacgggc acccgcctta cgctgcgcag aagctgggcc
     1801 gggtcatcac catcacgtcg ggcaaaggcg gcgtcggcaa gacgaccagc acggccaacc
     1861 tgggcacggc cctggcgctg ctgggcaaga aagtcgccgt catcgacgcc gacatcggcc
     1921 tgcgcaatct cgacgtggtc atgggcctgg aaaagcgcat cgtctacgac atcgtggacg
     1981 tggtcgaagg gcgctgccgc atccgccagg cgctgatcaa ggacaagcgc ttcccggagc
     2041 tgtacctgct gcccgccgcc cagacgcgcg acaagagcgc cgtcaacccc gcgcagatgc
     2101 gcttgctgtg cgaccacctc aaaaccgagt ttgattttgt gctgatcgac tgcccggcgg
     2161 gcatcgagca gggcttcaag aacgccatcg ccggggccga catggtggtg attgtcacca
     2221 cgccggaagt ctcggcggtg cgtgacgccg accgcgtgat cggctttttg gagacggcgg
     2281 gcatcaccga catccgattg atcgtcaacc gcatccgccc ggccatggtc aagggcggcg
     2341 acatgatgag cgtggacgac gtgctcgaca tcctcggcgc
//
LOCUS       DASUYC010000479         2676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_42805,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000479 DASUYC010000000
VERSION     DASUYC010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42805"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..2243
                     /locus_tag="VF276_07875"
     CDS             48..2243
                     /locus_tag="VF276_07875"
                     /inference="COORDINATES: protein motif:HMM:NF026092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HEX6606815.1"
                     /translation="MPSSMQQLVLKEGDIFIVTDEYGNIPENTPLGLYYHDTRYLSLL
                     NLKVNGTDPELLNSTSGQNFMSNLQFANTLFHLDGGTVVLPQTISIRRNRLVRGGLYE
                     RIGCMNYNRFPVTLTLLLEMGADFRDMFDVRGFARDRWGELRPPVLDDTGLHLSYLGL
                     DGVERQTHIRFSRPPDNAELRAAPSSQPTSSEAGIMIPVVDAEGRHPVIQAPRAVLTW
                     RVTLAPQHPFALSMHVYPESLGATEPEPPFDQAVQQMRRSYSGWQAHSSEITTSSESF
                     TRLLARSSWDLRVLSEELEQGYFPSAGIPWYACPFGRDSLITALQSLMLNPGLAVGTL
                     HVLAAHQGKVVDAWREEEPGKILHEVRFGEMARLQMVPHTPYYGTVDATPLFVMLFVE
                     TMRWLHDEQLYADLLPNVLRAIDWIDQYGDKDGDGFVEYVASRSNGGIRNQIWKDSDD
                     STQFPDGTLAETPIAACEVQGYVYAAKLGLSELLREHGDTARADRLAAEAATLRARFN
                     ERFWMPAANYYAQGLDARKVQIPTITSNPGHCLWAGICDEDKAEAVARRLIAPDMASG
                     WGVRTISSRSPSYNPMSYHNGSIWPHDNSIVIAGLRRYGRRDAALQAATQLYQAGLHF
                     RYYRLPELFCGFDRDDRYGAGPAEYPVSCSPQAWAAGAPLLLIQSLLGLEPGPAGEPP
                     RLDPWLPAWLTQVMIRHLRIGARRYQVEIQGGPDAPPVVHVGESLEEKD"
     gene            2381..>2676
                     /locus_tag="VF276_07880"
     CDS             2381..>2676
                     /locus_tag="VF276_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013145986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX6606816.1"
                     /translation="MATIFDVAEKAGVSITTVSRALNGYSDVNEETRKRVADAAAALN
                     YYPSAAARSLRGKRTNTIVFAPHLRARAESEPFFKEFTGALALACFKHDLSLLAT"
BASE COUNT          469 a          901 c          876 g          430 t
ORIGIN      
        1 tatgacggcc cggcgaaaca caatttgaag gaggtagcca gacccccatg cccagttcga
       61 tgcagcagtt ggtgctcaag gaaggtgaca tcttcatcgt caccgacgaa tacggcaata
      121 tccccgagaa cacgccgctc ggtctgtatt accatgacac gcgctacctc agcctgctga
      181 atctcaaagt caacggcacc gatcccgaac tactcaattc gaccagcggg cagaacttca
      241 tgagcaacct gcaattcgcc aataccctgt tccacctgga cggcggcacg gtggtgctgc
      301 cccagacgat cagcatccgg cgcaaccggc tggtgcgagg cgggttgtac gagcggatcg
      361 gctgcatgaa ctacaatcgc ttcccggtca cgctgacgtt gctgctggag atgggcgccg
      421 acttccggga catgttcgac gtgcgcggtt tcgcgcggga ccgctggggc gagttgcgcc
      481 cgcccgtgct cgacgatacg gggctgcacc tgagctacct ggggctggac ggggtggagc
      541 ggcagacgca tatccggttc agccgcccgc ccgacaacgc cgaattgcgc gcggctccct
      601 cctcgcagcc gacaagcagc gaggcgggca tcatgatccc ggtggtcgat gccgagggcc
      661 ggcaccccgt gatccaggcg ccgcgcgcgg tgctcacctg gcgcgtcacc ctggcgccac
      721 agcacccctt cgccctcagc atgcacgtct atcccgagag cctgggcgcg acggagcctg
      781 agccgccgtt cgaccaggcg gtgcagcaga tgcgccggtc gtacagcggc tggcaggcgc
      841 acagcagcga gatcaccacc tccagcgaaa gcttcacccg gctgctggcg cgcagcagtt
      901 gggatctgcg ggtgctgtcg gaggagctgg agcagggcta tttccccagc gccggcatcc
      961 cctggtacgc ctgcccattc gggcgcgaca gcctgatcac cgcgctgcaa tcgctgatgc
     1021 tcaatccggg gctggcggtg ggcacgttgc acgtgctggc cgcgcaccag ggcaaagtcg
     1081 tggacgcctg gcgcgaggaa gaaccaggca agatcctgca cgaggtccgc ttcggcgaga
     1141 tggcccgcct gcaaatggtg ccccacacgc cctattacgg caccgtggac gcgaccccgc
     1201 tcttcgtcat gctctttgtc gagacgatgc gctggctgca cgacgagcaa ctctatgccg
     1261 atctgctgcc caatgtactc cgcgccatcg attggatcga ccagtacggc gacaaagacg
     1321 gcgacggctt cgtcgagtat gtcgcgagcc ggtccaacgg cggcatccgc aaccagatct
     1381 ggaaggacag cgacgactcc acgcaattcc ccgacggcac gctggccgag acgccgatcg
     1441 ccgcctgcga ggtgcagggc tatgtctacg ccgccaagtt ggggctgagc gagttgctgc
     1501 gcgagcacgg cgacaccgcc cgcgccgacc ggctggccgc cgaggcggcc acgttgcggg
     1561 cgcgcttcaa cgagcggttc tggatgcctg ccgctaatta ttatgcccag ggcctcgacg
     1621 cgcgcaaagt ccagatcccg accatcacca gcaaccccgg ccactgcctc tgggccggca
     1681 tttgcgacga ggacaaggcc gaggccgtgg cccgccggct gatcgccccc gatatggcct
     1741 cgggctgggg cgtgcgcacg atcagcagcc gcagcccgtc gtacaacccg atgagctacc
     1801 ataacggctc gatctggccg cacgacaatt cgattgtcat cgccgggctg cgccgctatg
     1861 gccggcgcga tgcggcgttg caggccgcca cgcaactcta ccaggccggc ttgcacttcc
     1921 gctactatcg cctgcccgaa ctgttctgcg ggttcgaccg cgacgaccgc tacggcgcgg
     1981 gcccggcgga gtatccggtg tcgtgcagcc cgcaggcgtg ggcagcgggc gcgccgctgc
     2041 tgctgatcca gagtttgctc gggctggagc cggggccggc gggggagccg ccgcgcctcg
     2101 atccctggct gcccgcctgg ctgacccagg tcatgatccg ccatctgcgg atcggggcgc
     2161 ggcgctatca ggtggagatt caggggggcc cggacgcgcc gccggtcgta cacgtaggcg
     2221 agagcctgga ggagaaagac taagtccggg ttcttgacag gcggcgcggg ttctgccata
     2281 atgaagttat ccaaaacctt ttcggcatgc gccggtgggc gaattctgct gccgccacgc
     2341 ggcggcttgt gtgcaggccc tgtgacggga gctgacgagc atggcgacga tcttcgacgt
     2401 tgcggaaaaa gccggggtgt cgattaccac cgtgtcccgc gccctcaacg gctatagcga
     2461 cgtaaatgag gaaacgcgca aacgggtagc cgatgccgct gccgcgctca actactatcc
     2521 gagcgcggcg gcgcgcagcc tgcgcggcaa gcggaccaat acgatcgtct tcgcgcccca
     2581 cctgcgggcg cgggcggaat ccgagccgtt cttcaaggag ttcaccggcg ccctggcgct
     2641 ggcctgcttc aagcacgacc tgagtctgct ggcgac
//
LOCUS       DASUYC010000480         3523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_207720,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000480 DASUYC010000000
VERSION     DASUYC010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207720"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1593
                     /locus_tag="VF276_07885"
     CDS             <1..1593
                     /locus_tag="VF276_07885"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEX6606817.1"
                     /translation="RVDPAPAGKAAPATGGMAPGAEIHSGRPAPAANQAPLQAGQIDD
                     NARFGEYLDYLRQYQGPPVHPLPVDQRLFLRVVDGAQQPVAGARVQLSAQGRTVFDGR
                     TMSDGRVLFFPQMAGAGQVPQFDAVVSRGQQEVRTVVKPGGAEPVVSLPKLADNSGPV
                     GLDLVFLLDATGSMGDEIDQIKGTVDSIAGRIEQVPGSSPPRFGLVAYRDRGDEYVTR
                     SWDFTANVQQFSANLANVQAGGGGDTPESVNAGLHEAIHLPGWASADSGRHLRMIVLV
                     GDAPPHLDYANDYQYADLLQEAVQAGIKIFPIGASGLDDQGEYIFRQFAEITQGQFVF
                     LTYENGVAGAPGVSTDHHVSNFTVSQLDTLIVNLVAGEIANQTGQRAAVTDPASVPVP
                     VEGPDALPVAPPAPAWVGALTGLRDAVAGLAQDGSTLFWLLIILLVAVWMRRAPARAR
                     RAAAPPVSFAAPTEPDAEAPPTRSLESMAARVAPDRAAQRPAAVTAPPPAPVDAYLSV
                     EPGPGRTAPLPPLPTRAVERPR"
     gene            2026..>3523
                     /locus_tag="VF276_07890"
     CDS             2026..>3523
                     /locus_tag="VF276_07890"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEX6606818.1"
                     /translation="MLRNNRLFAVVAILGLLIVTVLGSGPGMALAAPAPGAPAPAATT
                     PVAPAAGCGMNFADVPAGSWFYPFVEYLYCSHAVSGYGSEFRPNAATTRAQLTKMIGN
                     ALGWPLNYTGAPHFADVPPAHPVYQTIEAAYAHGIISGYACGGAGEPCPGTYFRPGAD
                     VTRAQLAKMIILAQGWATANPAQPSFTDVPPGAWYAGYVEAANMQGVVSGYADGTFRP
                     FASATRAQLAKMIYNTLTPPAFDQGDNESEIQPQDDVSEETPEDLDPGPDGGPGLALP
                     GEAGKATSAPATAAISGTITSTNTGSPLVGARVVAHPVGGGADVETTTDAGGAYTLAV
                     PAGSYNVVAAAPDQMYPVTPAPGGPVTLADGGGATVSFALTQGGVLAGTITDSTTHAP
                     IQDADEWAVPVALPPRAYGGTLSDSQGHYRLVVPAGPVGAWAAKPSDTYPRTPPSSGN
                     PIAVTAGMTTTVDFALARGGVIAGHITDAATHAPLAGSRVWARHDPLAL"
BASE COUNT          509 a         1309 c         1216 g          489 t
ORIGIN      
        1 cgggtagacc cggcacccgc cggcaaagcg gcgcctgcga cgggcgggat ggcgccgggc
       61 gccgagatcc attcgggccg gcccgcgccc gccgccaacc aggcgccgct gcaggccggg
      121 cagatcgacg ataacgcccg cttcggcgaa tatctcgact atctccgcca gtaccagggg
      181 ccgccggtcc acccgctgcc cgtggaccag cggctatttc tgcgcgtggt cgacggcgcg
      241 caacagccgg tggccggggc gcgcgtgcaa ttgtccgcac agggccggac cgtcttcgac
      301 gggcgcacca tgagcgacgg gcgcgtgctg ttcttcccgc agatggcggg cgccgggcag
      361 gtgccgcagt tcgatgccgt ggttagccgc gggcagcaag aggtacgcac cgtcgtgaag
      421 ccgggcggcg ccgagccggt ggtgagcctg cccaaactgg ccgataacag cgggccggtg
      481 gggctggacc tggtcttcct gctggacgcg acgggcagca tgggcgacga gatcgaccag
      541 atcaagggca ccgtggacag catcgccggc cgcatcgagc aggtgccggg cagcagcccg
      601 ccgcgcttcg ggttggtggc ctaccgcgac cgcggcgacg aatacgtcac gcggtcctgg
      661 gacttcacgg ccaacgtgca gcagttctcg gccaatctcg ccaatgtgca ggccgggggc
      721 ggcggcgaca cgccggagtc ggtgaacgcg gggctccacg aggccatcca tctgccgggc
      781 tgggcgagcg ccgatagcgg gcgccacctg cgcatgatcg tgctggtggg cgacgccccg
      841 ccgcacctcg actatgccaa tgattaccag tatgccgatt tgctacagga ggccgtgcag
      901 gcgggtatca agatcttccc catcggcgcc agcgggctgg acgaccaggg cgagtacatc
      961 ttccgccagt ttgccgagat cacgcagggc cagttcgtct tcctgaccta cgagaacggc
     1021 gtggcgggcg cgccgggcgt gtccaccgac caccacgtct cgaacttcac ggtgagccaa
     1081 ctcgatacgc tgatcgtgaa tctggtggcg ggcgagatcg ccaaccagac ggggcagcgg
     1141 gcggcagtca ccgacccggc gtccgtccca gtgccggttg aggggccgga tgctctgccg
     1201 gtcgccccgc ctgcgcccgc ctgggtcggc gccctgaccg gcctgcgcga tgccgtggcc
     1261 gggctggccc aggacggcag caccctcttc tggctgctga tcattctgct ggtggccgtc
     1321 tggatgcggc gcgcccctgc ccgcgcccgc cgcgccgccg cgccgcccgt gtccttcgcc
     1381 gcgcctacgg agccggacgc cgaagccccg cccacgcgct cactggaaag catggccgcc
     1441 cgcgtggcgc ccgaccgcgc cgcccagcgg cccgccgccg tgaccgcacc gccgcccgcg
     1501 ccggtggacg cctacctcag cgtcgaaccg gggccggggc gcaccgcacc gctgccgccg
     1561 ctgcccaccc gtgcggtcga gcgcccacgc tgatcaggcc ggcacagcgt atctgtcact
     1621 ctgagcgcag cgaagagtct cgtcgtggac cccaacgaga cgagattctt cgctccgctc
     1681 agaatgacaa tgctcgtgtg atagcgtact gatagtgcca ccggcgacgg ggcgggatgg
     1741 cgatagacgc gccatcccgc ccctgcgttt gcctcagtcc gattgattca ccccccattt
     1801 caggctttta cggagatttt acacaggcat aacaggcccc ttacacccgc gcggtaggat
     1861 cgcgaaggta ggagtggccc ggcccgcgtg gtgggtaccg ggcgctccgc cgggcgccgc
     1921 tgttgcgtac cagccggcgc ccggcacttg cccccggcgg gcgtctgcgt tggtaccgcg
     1981 gaggcgccgc cggttcggcc cttcaacgtg gtaaggaggt tctgtatgtt gcgtaacaat
     2041 cgtcttttcg cggtggtcgc catcctcggc ttgctgatcg tgaccgtgct gggcagcggg
     2101 ccgggcatgg ccttggccgc gcccgcgccc ggcgccccgg cgcccgcggc cacgaccccc
     2161 gtcgcgcccg ccgccgggtg cggcatgaac ttcgccgatg tgccggcggg gagctggttc
     2221 tacccgtttg tcgagtacct ctattgctcg cacgccgtca gcggctacgg cagcgagttc
     2281 cgccccaacg ccgccacgac ccgcgcccaa ctgaccaaga tgatcggcaa cgcgctgggc
     2341 tggccgctca actacaccgg cgcaccgcac ttcgccgacg tgccgcccgc ccaccccgtc
     2401 taccagacca tcgaagccgc ctacgcccac ggcatcatca gcggctatgc ctgcggcggg
     2461 gcgggcgagc cgtgtcccgg cacgtacttc cgccccggcg ccgacgtgac ccgcgcccaa
     2521 ctggcgaaga tgatcatcct ggcccagggc tgggccacgg ccaatccggc ccagcccagc
     2581 ttcaccgacg tgccgcccgg cgcctggtac gccggctacg tcgaagcggc gaacatgcag
     2641 ggcgtggtca gcggctacgc ggacggcact ttccgcccgt ttgcctcggc cacccgcgcc
     2701 cagctcgcca agatgatcta caacacgctc accccgcccg ccttcgacca gggcgataac
     2761 gagtccgaga tccagccgca ggacgatgtg tcggaggaga cgccggaaga cctcgatccg
     2821 ggaccggacg gcgggccggg tctggccctg cccggtgagg ccggcaaggc gaccagcgcc
     2881 ccggcgacgg cggccatcag cggcacgatc acctcgacca acaccggcag tccgctggtt
     2941 ggggcgcgcg tggtggccca cccggtgggc ggcggcgcgg atgtcgaaac gaccaccgat
     3001 gccggcggcg cctacacgct ggccgtgccc gccggcagct acaacgtggt cgcggcggcg
     3061 cccgaccaga tgtacccggt gacgcccgcg cccggcggcc cggtcacgct ggccgacggt
     3121 ggcggggcga ccgtcagctt cgccctgacc cagggcggcg tgctggccgg tacgattacc
     3181 gatagcacca cccacgcgcc gatccaggac gcggacgagt gggccgtgcc ggtagcgctg
     3241 ccgccgcgtg cttatggcgg cacgctcagc gacagccagg gccactatcg gctcgtggtg
     3301 cccgccgggc cggtgggcgc ctgggccgcc aaaccgtccg atacctaccc gcgcaccccg
     3361 ccgagcagcg gcaatcccat cgccgtcacc gccggcatga cgaccacggt cgacttcgcc
     3421 ctggcgcggg gcggcgtgat cgccgggcac atcaccgacg cggcgaccca tgcgccgctg
     3481 gccgggtcgc gggtctgggc gcggcatgat ccgctggccc tcg
//
LOCUS       DASUYC010000481         2087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_701648,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000481 DASUYC010000000
VERSION     DASUYC010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701648"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="VF276_07895"
     CDS             <1..356
                     /locus_tag="VF276_07895"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEX6606819.1"
                     /translation="APTMFKHDGKYYLFYSANDYGGADYAVGYATCDSPTGPCQDAPE
                     NPILKSRLDPPLVIGPGHQALIQVADQTWIVYHAWEAVAGGRGDSRFMWIDRVNWPDG
                     KPQVQGPTTGPQPMP"
     gene            471..>2087
                     /locus_tag="VF276_07900"
     CDS             471..>2087
                     /locus_tag="VF276_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002618372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEX6606820.1"
                     /translation="MLGFVLSRGRWLALSVCMLALILLVAPAQAAGTQAAQPNSGTYT
                     NPLHIQIPGDGLVESCADPSIIRGQTPGDNYWYVYCTTDPLNDQDRNGSGGFNFHLIP
                     MLKSLDLVNWTYVGDAFASKPAWVAPTAGLWAPDIHYFNGQYYLYYTASDTSLPGGGS
                     AIGVATGPSPTGPWTDAGAPVVEPNGQWVFDPSVIEANGQRYIFYGSYFGGVRARTLS
                     PDGLHSDPASQTQITIPNRYEGTFIFKHDGYFYLFGSATNCCNGPLTGYSVFAGRSQN
                     VLGPYVDQEGVSLLAGRVGGTPVISMNGNRWVGPGHNAVFTDFDGQDWFLYHAVDRFD
                     PYFAGAVGFTKRPVLMDPLDWIDGWPTVRAGNWASDQPMPAPAAQPGQKDRYKPHPPN
                     LLQPGHEIAALSDEFNGTALSNQWTWVRPPAPATYSVGGGAFNFDTQAADLYVDSNNA
                     SVLTEPAPNGNYAVETKVTLNLPPEGCCFNYVQAGLVVYHDDDNFIKLASVSIWETRQ
                     TEFAKELNPVPAGYPRYGNTVVGPPGDSTWL"
BASE COUNT          374 a          722 c          644 g          347 t
ORIGIN      
        1 aggcgcccac catgttcaag cacgacggca agtactacct gttctattcg gccaatgatt
       61 atggcggcgc cgactatgcc gtgggctatg ccacctgcga ctcgcccacc ggtccctgcc
      121 aggatgcccc cgaaaacccc atcctgaaaa gccgcctcga cccgccgctg gtcatcggcc
      181 ccggccacca ggcgctgatc caggttgccg atcaaacgtg gatcgtctat catgcctggg
      241 aggcggtggc gggagggcgc ggcgacagcc ggttcatgtg gatcgaccgg gtgaactggc
      301 ccgacggcaa accgcaggtc cagggaccga ccaccggtcc ccaaccgatg ccctgagccg
      361 ggccggcgat gcccctttag gggcaggcgc gtgcctcgcc gggccgggta ttgactgccg
      421 gcgcatacct atggctgtat ggttacgccg ctatgcaaag gagcataacg atgctgggat
      481 tcgttctttc tcgtggccgc tggctcgcgc tttccgtatg catgctggcc ctgattctgc
      541 ttgtcgcgcc ggcgcaggcc gcaggcaccc aggccgccca gcccaactcc ggcacttaca
      601 ccaacccact gcatatccag atccccggcg acggcctggt cgaaagctgc gccgacccgt
      661 cgattatccg tggtcagacg cccggcgata actactggta tgtctactgc actaccgacc
      721 cgctgaacga ccaggaccgc aacgggagcg gcggcttcaa cttccacctg atcccgatgc
      781 taaaatcgct ggacctggtg aactggacat atgttggcga tgccttcgcg tccaagcccg
      841 cgtgggtggc gccgaccgcc gggctgtggg cgccggatat ccactatttc aacggtcagt
      901 actacctgta ctacaccgcg tccgatacct cgttgcccgg cggcggcagc gccataggcg
      961 tcgccaccgg ccccagcccg acaggcccct ggacggacgc cggggcgccg gtcgtggagc
     1021 cgaacggaca gtgggtcttt gacccgtcgg tgatcgaagc caacgggcag agatacatct
     1081 tctacggtag ctatttcggc ggcgtccggg ccaggacact ctctccggac ggactgcact
     1141 ccgacccggc cagccagacg cagatcacga tccccaaccg ctatgagggc acctttatct
     1201 tcaaacacga tgggtacttc tacctgttcg ggtcggccac caactgctgc aacggcccgc
     1261 tgaccggcta cagcgtcttt gccgggcgct cacagaacgt gctggggccg tatgtcgacc
     1321 aggaaggggt ctcgctgttg gccgggcgcg tgggcggcac cccggtgatc tcgatgaacg
     1381 gcaaccgttg ggtcggacca gggcataacg ccgttttcac cgacttcgac gggcaggact
     1441 ggttcctcta ccacgccgtg gaccgcttcg atccgtactt cgcgggcgcg gtcggcttca
     1501 ccaagcgccc ggtgttgatg gacccgctcg actggatcga cggctggccc acggtgcgcg
     1561 cgggcaactg ggcctcggat cagccgatgc cggccccggc ggcccagccc ggccagaaag
     1621 atcgctacaa gccgcatccg ccgaacctgc tgcagcccgg ccacgagatc gccgcgctca
     1681 gcgacgagtt caacggcacg gccctgagta accaatggac gtgggtacgt cccccggcgc
     1741 ccgccaccta cagcgtaggc ggcggcgcct tcaacttcga cacgcaagcc gccgacctgt
     1801 atgtggacag caacaacgcc tcggtgctga ccgagccggc gcccaacggc aactacgccg
     1861 tggaaaccaa ggtcacactg aacctgccgc cggagggttg ctgcttcaac tatgtgcagg
     1921 cgggcctggt ggtgtaccac gacgacgaca acttcatcaa gctggcatcc gtgtcgatct
     1981 gggagacgcg gcagacggaa ttcgccaagg aactcaaccc ggtgccggcg ggctacccgc
     2041 gctacggcaa caccgtcgtc gggccgccgg gtgactccac ctggctg
//
LOCUS       DASUYC010000482         2499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_887772,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000482 DASUYC010000000
VERSION     DASUYC010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887772"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..485
                     /locus_tag="VF276_07905"
     CDS             99..485
                     /locus_tag="VF276_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606821.1"
                     /translation="MDLTELLTGAVDKVPGAVAISLLGVDGVAVETITGSKPLPGEGN
                     GARPRASQAWEVELADLMLTARRAARSLQWGALRNITMETRQLTFLARMINPDYFLLL
                     VMERDANMGRARFELRRVGAALAQSL"
     gene            663..1286
                     /locus_tag="VF276_07910"
     CDS             663..1286
                     /locus_tag="VF276_07910"
                     /inference="COORDINATES: protein motif:HMM:NF025198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HEX6606822.1"
                     /translation="MSYGQPPSGNQGGSSNQGGYPPQGSGQGGYQPQGSGQGGYPPPS
                     GGGYQPPAGGGGYQPPPSGGGYQPPSGGGYQPPPSGGGYQPPSGGGYQPPGGYGAPVA
                     SSGSNVFAIISLIAGILSLPGACVGWGGIILGIAAIILGIVAMNQIKARGQGGRGLAI
                     GGIITGVIGLLLGGILLAIGAAALGFINSPEGRALLTSIPLTLTPQP"
     gene            complement(1345..>2499)
                     /locus_tag="VF276_07915"
     CDS             complement(1345..>2499)
                     /locus_tag="VF276_07915"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX6606823.1"
                     /translation="AILALLAAFALYALGSGWRPRGRALLWLAPYLGVTALASLLNAP
                     DRLTAVRLTGLVALVALGALLVYWCTDSPARFRAAVRIMIGLAGVEAVLAFAGLVAAW
                     FNSPLGVQPGRGEILVPFGTLWEPNVLGSYLAAGAVPALAALVAAPARRRAAGLAALL
                     ALILAALGLSLSRGAWAGFLAGAIVVAVGGWWLGRRTAARWPALAPRWPLAVGAGLAA
                     ALFLVAVAPALFPMTTKGLFNRANVAAYTPQADPSLRIRADMASQALAEVGAHPLIGH
                     GAGSFGVDHFDAQGRPGWISNLELHVLYDSGLVGLLALLGGVGVSAWAAWRALRHGDP
                     AARPAILGVLGAVAVLLVAYQATEASWLGYTWVYLGLLARAGTLGPEPAR"
BASE COUNT          393 a          912 c          876 g          318 t
ORIGIN      
        1 acctgttctg ctatactgcc gccgaggtgc agcgaaacgg gcctggagtg cgcgggaatg
       61 cggggtgccg gcgccacccc gaaaggagaa gctggagcat ggatctgacc gagctgctga
      121 ccggcgctgt cgataaagtc cccggcgccg tggcgattag cctgttgggg gtcgacggcg
      181 tggcggtcga aacgatcacc gggtccaaac cgctgccggg cgagggcaac ggtgcccggc
      241 cccgcgcatc ccaggcctgg gaagtcgaac tcgcggatct catgctgacc gcgcggcggg
      301 cggcgcgcag cctccagtgg ggcgccctgc gcaacatcac catggagacc cgccagttga
      361 ccttcctggc gcgcatgatt aaccccgact acttcctgct gctcgtcatg gaacgggacg
      421 cgaacatggg ccgcgcccgc tttgaactgc ggcgggttgg cgccgccctc gcccagagcc
      481 tctagcgcgg gccgctctgt cgatccgtgc gcccggcgcc ggcgatccat gccctttgcg
      541 cgcaacacgg ccctggcctg catcgtatta atacgcatga atcgcgtgca agccgctccg
      601 cgcggacgca cagcagccga gacacgacgg tagttccgct tattttcagg agggacgatc
      661 acatgagtta tggacagcct ccgtccggca accagggcgg atcgagcaat cagggcggct
      721 atccgccgca agggtcgggc cagggtggct atcagcccca ggggtcgggc cagggcggct
      781 atccgccgcc gagcgggggc ggctaccagc ctccggcggg gggcggcggc tatcagcctc
      841 cgccgagtgg tgggggttac cagccgccga gtggcggtgg gtatcagccg ccgccgagtg
      901 ggggcgggta tcagccgccg agtgggggcg ggtatcagcc gccgggcggc tatggcgccc
      961 cggtcgcgag cagcggcagc aatgtcttcg cgatcatctc gctcatcgcc gggatcctgt
     1021 cgctgccggg cgcctgcgtc ggctggggcg gcatcatcct gggcatcgcc gcgattatcc
     1081 tcggcattgt cgccatgaac cagatcaagg cgcgcggcca gggcgggcgc gggttggcga
     1141 tcggcggcat catcaccggt gtgatcggcc tgctgctggg cgggattttg ctcgccatcg
     1201 gcgccgccgc gctcgggttc atcaactcgc cggaggggcg cgccctcctg acctcgatcc
     1261 cgttgacgct gacgccgcag ccgtaagcca atacgtatct cgacacatga ccgcctgggg
     1321 ctgctgctcc gggcggttcg ctttttaccg cgccggttcc ggccccaggg tgccggcacg
     1381 cgccagcaac cccaggtaga cccaggtgta gccgagccag ctcgcctcgg tcgcctggta
     1441 ggccaccagc agcacggcga cggcgccgag cacgcccagg atggccgggc gggccgccgg
     1501 gtcgccgtgg cgtagcgcgc gccacgccgc ccacgccgaa acccccaccc cacccagcaa
     1561 cgccagcaac cccaccaggc cgctgtcgta gagcacatgg agttccaggt tgctgatcca
     1621 gccggggcgg ccttgcgcgt cgaagtggtc cacgccgaag ctgcccgcgc cgtggccgat
     1681 cagcgggtgc gcgccgacct cagccagcgc ctggcttgcc atatcggcgc ggatgcgtaa
     1741 cgagggatcg gcctgcggcg tgtaagccgc gacgttggcc cggttgaaca accccttggt
     1801 ggtcatgggg aagagcgcgg gcgccacggc gacgagaaac agtgcagcgg ccagcccggc
     1861 ccccaccgcc aggggccatc tcggtgccaa cgcgggccac cgcgctgccg tgcgccgccc
     1921 cagccaccat ccgccgacgg caaccacgat ggcgcccgcc aggaacccgg cccaggcgcc
     1981 ccgcgacaga ctcagcccca gtgccgccag gatcagcgcg agcagggcgg cgaggccggc
     2041 ggcccggcgg cgagcgggcg cagctaccag ggccgccagc gccggcaccg cgcccgcggc
     2101 caggtagctg cccaggacat tcggctccca cagcgtgccg aagggcacca ggatctcgcc
     2161 ccgtcccggc tgcaccccca gcgggctatt gaaccaggcc gcaaccagcc cggcgaacgc
     2221 caggaccgcc tctaccccgg cgaggccaat cataatgcgc acggcggccc gaaaccgcgc
     2281 cgggctatcg gtacaccagt agaccagcag cgcgcccagc gccaccagcg ccaccaggcc
     2341 ggtgaggcgc acggcggtga ggcggtccgg cgcgttcagc agcgaggcca gcgccgtcac
     2401 gcccaggtag ggcgccagcc acagcagcgc ccgcccgcgg ggccgccacc cgctgcccag
     2461 cgcatagagg gcgaacgccg ccagcagcgc caggatcgc
//
LOCUS       DASUYC010000483         2459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_697626,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000483 DASUYC010000000
VERSION     DASUYC010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697626"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1097
                     /locus_tag="VF276_07920"
     CDS             <1..1097
                     /locus_tag="VF276_07920"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX6606824.1"
                     /translation="VDLPQPVRAGEAFTATIAYAGAPSTVVPEALQIGTGWQRSETGL
                     FVMGEPAGAEGWYAVNDHPCDKATYTFNVTVPKPYVVAANGLLADTRDNGATQTYRWE
                     MRDPMASYLATVDIDDFVVQEVDGPDGLPIRNYFPRAIATEAEETFAHTAEMIAFFST
                     RFGPYPFDAYGVAVANSDLNYALESQTLSLFGRSLVRDSRYPDPVVAHELAHQWFGDS
                     VSLRQWQDIWLNEGFATYAEVLWAENVGGAAGRDDYVRNLYRQVTDRTYPPPGKPPAD
                     NLFNRGVYLRGGLTLHALRLKVGDATFFRILQTYTARYRYGNAGTADFIAVAQEVSGQ
                     DLQAFFDSWLYAKAIPDIPELDLRAPTPVP"
     gene            1123..1530
                     /locus_tag="VF276_07925"
     CDS             1123..1530
                     /locus_tag="VF276_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606825.1"
                     /translation="MKTRNILIAVAALAVALAVGFGALAMGGNPPLPANGGTPTPAPG
                     GAPAALTARAVADLSKQLGIPEANIKVASSEAVEWSDGSLGCPQPGMSYAQVITPGYR
                     IVLQAGDKQYEYHTGEGDAAQVIHCTAGAGGNK"
     gene            complement(2069..>2459)
                     /locus_tag="VF276_07930"
     CDS             complement(2069..>2459)
                     /locus_tag="VF276_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606826.1"
                     /translation="ARPLWDRSAVLGRLLLDRFYWLTAGFGYRPLRAVLINSVVVTAF
                     AYIYWQFHLLCIFYVQGGQNSVPQCQDVSFLQSLYFSALAFFLAAMGEILPRPLWGQA
                     LLVLESIWGFLNVSVIIAVIINRQSGE"
BASE COUNT          454 a          781 c          829 g          395 t
ORIGIN      
        1 cggtggatct gccccagccg gtgcgcgcgg gcgaggcttt cacggccacg attgcctacg
       61 cgggcgcgcc ttccaccgtg gtgccggaag cgttgcagat cggcaccggg tggcagcgct
      121 cggagaccgg gctgttcgtc atgggcgagc cggcgggcgc cgaggggtgg tacgcggtga
      181 acgaccaccc atgcgacaag gcaacctaca ccttcaacgt cacggtgccg aagccgtacg
      241 tcgtggcagc caacggcctg ctggccgata cgcgcgacaa cggcgccacg cagacctacc
      301 gctgggagat gcgcgacccg atggcctcat atctcgccac ggtggatatc gacgactttg
      361 tggtgcaaga ggtggatggc cccgatgggc tgccgatccg caactacttc ccgcgggcga
      421 tcgccaccga ggccgaggag actttcgccc atacggcgga gatgatcgcc ttcttcagca
      481 cgcgcttcgg cccgtatccg tttgacgcct acggcgtggc cgtggcgaac agcgacctca
      541 attatgcgct ggagtcacag acgctgtcgc tgttcgggcg cagcctggtc cgcgactccc
      601 gctatcccga cccggtggtg gcccacgaac tggcccacca gtggttcggc gacagcgtca
      661 gcctgcgcca gtggcaagac atctggctga acgaggggtt cgcgacgtat gccgaggtgc
      721 tctgggccga gaatgtgggc ggcgccgccg ggcgggacga ctatgtgcgc aatctctacc
      781 gccaggtgac ggatcgcacc tacccgccgc cgggcaaacc gcccgccgat aatctgttca
      841 atcgcggcgt atacctgcgt ggcgggctga ctctgcacgc gctgcggctg aaggtcggcg
      901 acgccacctt cttccgcatc ttgcagacct acaccgcacg ctaccgctac ggcaacgccg
      961 gcaccgccga cttcatcgcc gtggcgcagg aggtgagcgg gcaagacctg caagccttct
     1021 ttgatagctg gctctatgcc aaagccatac cggacatccc tgaactggac ctgcgggccc
     1081 cgaccccggt cccttagttt cgagacatgg aggaatcgac ctgtgaaaac ccgtaatatc
     1141 ctgatcgccg tcgcggccct ggccgtggcg ctggccgtgg gcttcggcgc cctggcgatg
     1201 ggcggcaacc cgccgctgcc cgcgaacggc ggcacgccca cccctgcgcc cggcggcgcc
     1261 cccgcagcgc tgaccgcgcg agccgtcgcc gacctgagca agcagcttgg catcccggag
     1321 gcgaacatca aagtcgcttc gagcgaggcc gtggaatggt cggacggcag cctgggctgc
     1381 ccccagccgg gcatgagcta cgcgcaggtg atcacaccgg gctaccggat cgtgctgcaa
     1441 gcgggcgaca agcagtatga gtaccacacc ggcgagggcg acgccgccca ggtgatccac
     1501 tgcaccgccg gcgccggcgg gaacaagtag cttccgcccc cgttccctgg accggtgccc
     1561 gcctttgccc aattgcgaag gtgggcaccg ctttatttta agatgccgat agactcatag
     1621 aatcacacct tagcctgtgg gtctattagc gtacaagtgt actagcatag tagcttgctc
     1681 gctcaagctt tcttgatctg gcgaaatgga aaaggcagtg cgcgatcaga tagagtgcct
     1741 cacggtaagc ggggcggcgg gcaggcatct ggacggtagt aagccacctg attttgcggt
     1801 gtaggggccg gtttgaaacc ggcctggctc ccgtcagagt ccgtgcccgc gccggtaacg
     1861 ggttgccggc taggcgagat cagggtagga cggcaagcgc gataaatcgc gctgctacgg
     1921 agacgcccgc ggtggacggc aagcgcgata aatcgcgctg ctacggtcgg gcatcagcgc
     1981 cgttggctgg tgttatggcc gatgatatga ttatgacacg atcacggagg caggcacgca
     2041 gcgttggcgc tagactggcg ggcctgggtt actcgccgct ctgccggttg ataatgacgg
     2101 cgatgatgac cgagacgttc aggaagcccc agatgctttc gaggaccagc agggcctggc
     2161 cccagagcgg gcggggcagg atctcgccca tcgcggcgag gaagaaggcg agagcgctga
     2221 agtagagcga ttgcaggaag ctcacgtcct ggcattgggg caccgagttc tgcccgccct
     2281 ggacatagaa gatgcagagc aggtggaact gccagtagat ataggcgaag gcggtgacca
     2341 ccacactgtt gatcagcacg gcgcgcagcg ggcggtagcc gaacccggcg gtgagccagt
     2401 agaaccggtc gagcagcagg cggcccagca ccgccgagcg atcccagagc gggcgcgcg
//
LOCUS       DASUYC010000484         2197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1487320, whole genome shotgun sequence.
ACCESSION   DASUYC010000484 DASUYC010000000
VERSION     DASUYC010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487320"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..237)
                     /locus_tag="VF276_07935"
     CDS             complement(52..237)
                     /locus_tag="VF276_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606827.1"
                     /translation="MATPRFTGKGGAGAALALAGVITDRPGHIGPLYPDVAAYRASEL
                     VQRAIRTTGAGVMGRGA"
     gene            complement(441..1037)
                     /locus_tag="VF276_07940"
     CDS             complement(441..1037)
                     /locus_tag="VF276_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX6606828.1"
                     /translation="MRKIVVGMFVSLDGVMEGPGPADDFPLAGWTMPYWDADVAEFVD
                     AAMGTSDTLLLGRITYQGFAAAFGDAPADNPDAATMNRFTKYVVSTTLEQAEWANSTV
                     IRENVVEEITRLKEQPGRDIAISGSGQVVHTLMRANLIDEYRLLIYPVILGQGKRLFP
                     PDVPVTLDLVEARTSAKGVLLVTYRPAEGPDAAGAGGG"
     gene            complement(1123..1998)
                     /locus_tag="VF276_07945"
     CDS             complement(1123..1998)
                     /locus_tag="VF276_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019586939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6606829.1"
                     /translation="MAESVLEHRMGADTAGAGEYAPVNGLNLYYERYGSGRPLVLLHG
                     GFGVIGQMFGHLIPLLAAQRQVIAVELQGHGHTGDIDRPLSFEALADDIAALLRHLGL
                     AQADLCGYSLGGGVALQTAIRHPALVGKLVLISAPARRQGWYPATLAGMAAVNGVAAA
                     TWVGSPMHAAYAAVAPNVDAWPALADKLQALLGQEYDWSAQVGALPMPVLIVVGDADA
                     VRTAHAVEMFALLGGGQQPAGLGVPLPQGRLAILPGTTHYDIIFRTDLLVPILTTFLA
                     AAPAEAMREPPAEGR"
BASE COUNT          348 a          866 c          656 g          327 t
ORIGIN      
        1 tgcatcgccc cctgccgcca gcgacggtct gacaggcgcg cccgcagccg tttaggcgcc
       61 gcgccccatc acgcccgccc cggtggtgcg gatggcccgc tgcaccaact cgctcgcccg
      121 gtacgccgcg acatcaggat agagcgggcc gatatggccg gggcggtcgg tgataacgcc
      181 ggccagcgcc agcgccgccc ccgcgccgcc cttgcccgtg aaacgaggtg tggccataga
      241 aacgcgccct ccggcggccg gtattgcgtg tccaccgcga tccccggccg gatcgccgcc
      301 tgccccgccc gccgtgccgt ggaccgcaaa cgccccgtgc cgccgcagca ccgggcggcc
      361 ctcgccgccg tgcatttcgt cgtacatcgg aagaccgttg aggggcgcac cgcccgtctg
      421 cggcgcggga ggagtcatgg tcatcccccg cccgctcccg ccgcgtccgg cccctcggcc
      481 gggcggtagg tcacgagcag cacgcccttc gcgctcgtcc gcgcctccac caggtccagc
      541 gtcaccggca catccggcgg gaacagccgc ttgccttgcc ccaggatcac cgggtagatc
      601 agcagccggt actcatcaat caggttggcg cgcatcagcg tgtggaccac ctgcccgctg
      661 ccgctgatcg caatgtcccg ccccggctgt tccttcagcc gggtgatttc ctccaccaca
      721 ttctcgcgga tcaccgtgga attggcccac tcggcctgct ccagcgtggt cgacaccacg
      781 tacttggtga agcgattcat cgtggccgca tccggattat cggcgggcgc gtcgccaaag
      841 gcggcggcga acccttgata ggtgatccgt cccagcagca aggtgtcact cgtgcccatg
      901 gctgcgtcca cgaactccgc cacgtccgca tcccagtagg gcatcgtcca cccggcgagc
      961 gggaaatcat cggccgggcc tggcccttcc attaccccat ccagcgacac aaacatcccc
     1021 acaacaattt tccgcacagt aaactcctcg tagggggcac ggaccgtccg cctccccgcg
     1081 gttgcgcgcg gacggccctg gggccgcccc cgcgttgcca tgctagcggc cctcggccgg
     1141 tggttccctc atcgcctccg cgggcgcggc agcgaggaag gtcgtcagga tcggcaccag
     1201 cagatcggtg cggaagatga tgtcataatg cgtggtgccc ggcaggatgg ccagccgccc
     1261 ctgcggcagc ggcacgccca gccccgccgg ctgctgcccg ccgcccagca gggcgaacat
     1321 ctccaccgcg tgggccgtgc gcaccgcgtc ggcatccccg accacgatca gtaccggcat
     1381 cggcagcgcg cccacctggg ccgaccagtc atattcctgc ccgagcaggg cctgcagctt
     1441 gtccgccagc gccggccacg cgtccacgtt cggggccacg gcggcatacg ctgcgtgcat
     1501 cgggctgccc acccaggtcg ccgccgccac cccattgacc gccgccatgc ccgccagcgt
     1561 cgcgggatac cagccctgcc gccgggccgg ggccgagatg agcaccagtt tgcccaccag
     1621 cgcggggtgg cgtatggccg tttgcagcgc cactcccccg cccagcgaat acccgcacag
     1681 gtcggcctgc gccaggccca ggtgccgcag cagcgccgcg atgtcgtcgg ccagcgcttc
     1741 aaagctcagc gggcgatcaa tatcccccgt atgtccgtgc ccctgcaact ccaccgcgat
     1801 cacctgccgc tgcgcggcca gcagcgggat caggtgcccg aacatctgcc caatgacccc
     1861 gaacccgccg tgcaacagca ccagcggccg gccgctccca taacgctcat agtataggtt
     1921 cagaccgttg accggcgcat actcgcccgc ccccgccgtg tccgccccca tgcgatgctc
     1981 caaaacgctc tccgccatga tcacaactcc tttgtgttag tcgctcgcag tatacgcgag
     2041 aacaactaaa gcaggtccgt cggcggggcg ttagccccat aacgcgtccc ggccccgcgc
     2101 ccgcccggat atcgatctga tccccgctaa aatccccgat ccagggccca taacaaaaac
     2161 caaacgggtc gtgtagtccg tggccccttt accttat
//
LOCUS       DASUYC010000485         6084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1289849, whole genome shotgun sequence.
ACCESSION   DASUYC010000485 DASUYC010000000
VERSION     DASUYC010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6084
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289849"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VF276_07950"
     CDS             <1..492
                     /locus_tag="VF276_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX6606830.1"
                     /translation="LLEAAKLGDIFITVTGNLNVIDRHDMEVMKSGAILANSGHFNDE
                     INIPALRELSSSVRTVRDFVEEFTLQDGRRIYLLGEGRLINLAAAEGHPASVMDMSFA
                     NQSLAAEYLVQHQGELQNQVYTIPAELDQEIALLKLRAMGIEIDVLTEEQQKYLASWE
                     SGT"
     gene            514..1140
                     /locus_tag="VF276_07955"
     CDS             514..1140
                     /locus_tag="VF276_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606831.1"
                     /translation="MTTCANHPDVAAMGACRQCGRPLCSLCVYFRDGQILCAAHNAAA
                     GDPAAPSSDVTTGAVPGPIQGEPVARPPANIDNGPVAPAWAIDPRLEPLPMSMRPGWT
                     DEPLEPRRSGNAETFGLIGLIGSLVSLPFGICCGVGGVVGIPLALIAGGLCIAALVLA
                     PRGRNPSSARWMGGVGLGFAALTLAVSLCFFAVTFAGLGTSLFSIATP"
     gene            1155..2420
                     /gene="metK"
                     /locus_tag="VF276_07960"
     CDS             1155..2420
                     /gene="metK"
                     /locus_tag="VF276_07960"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX6606832.1"
                     /translation="MMTTFTESPQFFFTSESVTEGHPDKLCDQISDAILDAMLDQDPN
                     SRVACETATTTGMVLVMGEVTTKSWVDIPKLVRDTINDIGYTRAKYGFDAETCNVIVS
                     IKEQSKDIAQGVDEALEHKTGEMTDAEVEALGAGDQGMMIGFACDETPELMPLTISLA
                     NGLTRRLSQLRRSKVLPWLRPDGKSQVTVEYSYGKPKRVDAVVVSTQHAPHVSHEEIE
                     REIIEKVIRAVVPAELLDDQTKFYVNPTGRFVIGGPMGDAGVTGRKIIVDTYGGVARH
                     GGGAFSGKDPTKVDRSAAYAARYVAKNVVAAGLAERFEIQLSYAIGVAHPISISVETF
                     GTGKVSDDVIVELIKKHFDLRPGAIIRDLKLRRPIYRATAAFGHFGRTDIDAPWENTD
                     KAAILRAEAGLGESGSDTSAGRQLVGSRG"
     gene            2450..3496
                     /locus_tag="VF276_07965"
     CDS             2450..3496
                     /locus_tag="VF276_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX6606833.1"
                     /translation="MRALITGAGGFAGRYLVAHLAQVTGWELWGTTRPHKAATPEGST
                     PGEPSGRRRDTGRRPLDSTWATQHRIRMAPVDLLDYAGTRDLLAELRPDFIFHLAAQA
                     IVQQALRDPQGTLVNNVVGELNVLRAVQELGLAPRILLIGSSEQYGHVRPEDLPVNED
                     TPFRPENPYAVSKIAQDMLGLQYFVAYKLPIIRVRPFNHIGPGQSEQFVTAAFARQVA
                     AIEAGLQEPILRVGNLSAERDFTDVRDMVRAYHLAITEGEPGAVYNIGSGTATAISTI
                     LDTFLSMSKVAVQIERDPARLRPADVPRIVCDASRFRERTGWQPEIGLQQSLRDILND
                     WRERVTDVLDGGNG"
     gene            3699..4748
                     /locus_tag="VF276_07970"
     CDS             3699..4748
                     /locus_tag="VF276_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX6606834.1"
                     /translation="MRVLITGITGFAGSHMADMLFGKPDIELFGTYRRRSRLDNLETL
                     VPHLNLIEVGVSSVDTIRAAFVSGKINMINSDLVDAFSVHKLIAAVRPDRIFHLAAQS
                     FVPTSWNAPAETLLTNTMGQLNLFEAVRETRALAAEQGQGAFDPLIQIAGSSEEYGLV
                     FPNEVPMTEANELRPLSPYAVSKVTQEKLAYQYWMSYQVKSIVSRGFNHDGPRRGEHF
                     APSTWARQIARIEKGLQEPIVYVGDLTSKRDITDVRDMVRAYWLMLERGTPGEVYNIG
                     SGQTRSMGEMLDMLLSMSKARIEIKQDPARMRPSDVKILWADAGKFQKATGWEPTIPF
                     EQTIRDLLAYWRAHV"
     gene            5095..5574
                     /gene="nrdR"
                     /locus_tag="VF276_07975"
     CDS             5095..5574
                     /gene="nrdR"
                     /locus_tag="VF276_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEX6606835.1"
                     /translation="MRCPYCHKGESQVLDTRETPDSDGIRRRRRCNNCGQRFTTYERV
                     QPVNLMIVKKEGLREEYNRGKIESGIRLACTKRPVSATTIENAVNSVESELFGLGQNE
                     VPAKVVGDLVLNRLREIDQVAYIRFASVYLNFADLAEMGQAIEALLQRDTRSERSPA"
     gene            5623..>6084
                     /locus_tag="VF276_07980"
     CDS             5623..>6084
                     /locus_tag="VF276_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HEX6606836.1"
                     /translation="MAKTKPASPGTTHQPDVGVPLAKNAVTVLERRYLVKDEAGKVIE
                     TPDEMFHRVARNLAEAEGRYGADVDLWEDRFYRLMRSLEFLPNSPTLMNAGRPLQQLS
                     ACFVLPVGDDLGEIFSTIRNQALIHQTGGGTGFAFSRLRPKNDIVMTTKGKA"
BASE COUNT         1087 a         1964 c         1992 g         1041 t
ORIGIN      
        1 ctgctggagg ccgccaagct cggcgacatc ttcatcaccg tgaccggcaa cctcaatgtg
       61 attgaccgcc acgacatgga ggtgatgaag agcggcgcta tcctcgcgaa cagcggccac
      121 ttcaacgacg agatcaacat cccggccctg cgcgagctgt ccagcagcgt ccgcaccgtg
      181 cgcgatttcg tcgaggagtt cacgctgcag gacgggcgcc gcatctacct gctgggcgaa
      241 ggccgcctga tcaacctggc cgccgccgaa ggccacccgg ccagcgtgat ggacatgagc
      301 ttcgccaacc agtcgctggc tgccgagtat ctggtccagc accagggcga gctccagaac
      361 caggtctaca ccatcccggc ggagttggac caggagatcg ccctgctgaa gctgcgcgcg
      421 atgggtatcg agatcgacgt cctgaccgag gagcagcaga agtatctggc ctcctgggaa
      481 tccgggacct aagcgtagcg ttgcgaatcg cgagtgacga cctgcgcgaa tcacccggat
      541 gtggcggcga tgggcgcgtg ccggcaatgt ggccggcccc tctgctcgct gtgtgtgtac
      601 tttcgggacg gccagatcct ctgcgcggcg cacaacgccg ccgccgggga tccggccgcc
      661 ccctcaagcg acgtgacgac cggcgcggtc cctggcccga tccagggcga accggtcgcc
      721 aggccgcccg cgaacattga caatgggccg gtggcccccg cctgggcgat cgatccccgg
      781 ctggagccgc tgccgatgtc gatgcgcccc ggctggaccg atgagccgct ggagccgcgg
      841 cgcagcggca atgccgagac gttcggcctg atcgggctga tcggcagcct ggtgtcgctc
      901 ccgttcggga tctgctgcgg cgtgggcggg gtcgtgggca taccgctggc cctcatcgcc
      961 ggggggctgt gcatcgccgc tctggtgctc gcgccgcgcg ggcgcaaccc gtcttcggcg
     1021 cgctggatgg gcggagttgg ccttgggttt gccgcgctga ccttagccgt cagcctatgt
     1081 tttttcgccg tgaccttcgc cggcctcggc acatcgcttt ttagtatcgc aacgccgtaa
     1141 cgcgtttgga ggacatgatg acaactttta cggaatcgcc gcagtttttc ttcacctcgg
     1201 agtcggtgac ggaaggccac cctgataagc tgtgcgacca gatctcggac gcgatcctgg
     1261 acgccatgct ggatcaggat ccgaacagcc gcgtggcctg cgaaacggcg accaccaccg
     1321 gtatggtcct ggtcatgggc gaggtcacca ccaagagctg ggtcgatatt cccaaactgg
     1381 tgcgcgacac gatcaacgat attggctaca cgcgcgccaa gtacggtttt gacgctgaga
     1441 cgtgcaacgt gattgtttcg atcaaggagc agtccaagga catcgcccag ggcgtggacg
     1501 aggccctgga gcacaagacc ggcgagatga ccgacgccga ggtcgaggcg ctgggcgccg
     1561 gcgaccaggg catgatgatc ggctttgcct gcgacgagac gcccgaactg atgccgctga
     1621 ccatctcgct ggcgaatggg ctgacccgcc gcctgtcgca gttgcgccgc agcaaggtgc
     1681 tgccctggct gcgccccgac ggcaagtccc aggtcacggt tgagtacagc tacggcaagc
     1741 ccaagcgggt ggacgccgtg gtcgtttcga cccagcacgc gccgcacgtc agccacgagg
     1801 agatcgagcg agagatcatc gagaaggtga tccgcgccgt ggtgcccgcc gaactgctcg
     1861 atgaccagac caagttctac gtgaacccga ccgggcgctt cgtgatcggc ggcccgatgg
     1921 gcgatgccgg cgtgaccggg cgcaagatca tcgttgatac ctacggcggg gtggcccgcc
     1981 acggcggcgg cgccttcagc ggcaaggacc cgaccaaggt ggatcgctcg gcggcctatg
     2041 ccgcgcgcta tgtggcgaag aacgtcgtcg ccgccggtct ggccgagcgc ttcgagatcc
     2101 agctcagcta tgccatcggc gtggcccacc cgatcagcat ctcggtcgag acgttcggca
     2161 ccgggaaggt gtccgacgat gtcatcgtcg aattgatcaa gaagcacttc gacctgcggc
     2221 ccggcgctat catccgcgac ctgaagctgc gccgcccgat ctatcgcgcc accgccgcct
     2281 tcgggcactt tggccgcacc gatatcgacg cgccgtggga gaatacggac aaggccgcga
     2341 tcctgcgcgc ggaagccgga ctcggcgagt cgggtagcga cacctccgcc ggccgccaac
     2401 tggtcggttc gcgcggttga acccgctctc gcccactgcg gagcgaccga tgcgcgcatt
     2461 gatcaccggg gccgggggct tcgcggggcg ctacctggtg gcgcacctcg cgcaggtcac
     2521 cggctgggag ttgtggggga ctacgcgccc ccacaaagcc gcaacgccgg agggttccac
     2581 gcctggggaa ccctccgggc gccgccgcga caccggacgg cgcccgctgg acagtacctg
     2641 ggccacgcaa caccggatcc ggatggcgcc ggtggacctg ctggactacg ccggcacgcg
     2701 cgatctgctg gcggagctgc gccccgactt tatctttcac ctggcggcgc aggctattgt
     2761 gcagcaggcc ctgcgcgatc cgcagggaac gctggtcaac aatgtcgtcg gcgaattgaa
     2821 cgtgttgcgc gcggtgcagg agttgggcct ggcgccgcgc attttgctga tcggcagcag
     2881 cgagcaatac gggcatgtgc ggcccgagga tttgcccgtt aacgaagata cgccgttccg
     2941 cccggagaat ccctacgcgg tcagcaagat cgcgcaggat atgctgggcc tgcagtattt
     3001 tgtggcttat aagctgccga tcatccgcgt gcgtccgttt aatcatatcg gccccggcca
     3061 gagcgagcag tttgtaaccg ccgccttcgc ccgccaggtg gccgctatcg aggccggttt
     3121 gcaggagccg atcctgcggg tgggcaacct gagcgccgaa cgggatttca ccgatgtgcg
     3181 cgatatggtg cgcgcgtatc acctggcgat cacggaaggc gaacccggcg cggtgtataa
     3241 tatcggttca ggcacggcca ccgcgatctc cacgatcctc gatacctttc tcagcatgag
     3301 caaggtcgcg gtgcagatcg agcgcgatcc ggcccgcctg cgcccggcgg atgtgccccg
     3361 catcgtgtgt gatgcgagcc ggtttcgcga gcgcacgggg tggcagccgg aaatcggcct
     3421 gcagcagtcg ctgcgcgata tcctgaacga ttggcgcgag cgcgtgacgg acgtgctgga
     3481 cggcgggaac ggctgaccgg gcacggcgca ttcccggtgc cgggtgtggg ccctgttgca
     3541 gtcgatcccg cccgccttgc atatgataga ggtgcaagcc tggtagccat gggccggtgc
     3601 ggcggaccgg cccccgcctt gtggctaatg cgctcgtgcc ggcgcccggt aggaccaccg
     3661 gcagcgtttt aaaactggta acaaacggag gaaacccctt gagagttctg attactggta
     3721 tcaccggttt tgccggcagc catatggcgg acatgctttt tggcaagccc gatatcgaat
     3781 tgttcggcac ctaccggcgg cgcagccggc tcgataacct ggagaccctg gtgccgcacc
     3841 tcaatctcat cgaggtcggc gtctccagcg tggatacgat tcgcgcggcg tttgtgtccg
     3901 gcaagatcaa catgatcaac agcgacctcg tggatgcctt cagcgtgcat aagctgatcg
     3961 ccgccgtgcg cccggatcgc atctttcacc tggccgcgca gtcctttgtg ccgacatcgt
     4021 ggaatgcccc cgccgaaacg ctgctgacca acaccatggg ccagctcaat ctcttcgagg
     4081 cggtgcgcga gacccgtgcc ctggcggctg agcaagggca gggcgcgttc gatccgctga
     4141 tccagatcgc cgggtcgtcg gaggagtacg gcctggtgtt ccccaacgaa gtgcccatga
     4201 cagaggccaa cgagttgcgc ccgctcagcc cctacgcggt gagcaaggtg acgcaggaga
     4261 agctggccta ccagtattgg atgagctacc aggtcaagag catcgtctcg cgcggcttca
     4321 accacgacgg cccgcgccgc ggcgagcact ttgcgccttc cacctgggcc cgccagatcg
     4381 cgcgcatcga gaagggcttg caggaaccga ttgtgtatgt gggcgacctg accagcaagc
     4441 gcgacatcac cgatgtgcgc gacatggtgc gcgcctactg gctgatgctg gagcgcggca
     4501 cgcccggcga ggtctacaac atcggctcgg gccagacccg cagcatgggc gagatgctcg
     4561 atatgctgct gagtatgagc aaggcgcgca tcgagatcaa gcaggacccg gcccgcatgc
     4621 gccccagcga cgtgaaaatc ctctgggccg acgccggcaa attccagaaa gctaccggct
     4681 gggagccgac cattcccttc gagcagacca tccgcgatct gctggcctac tggcgcgccc
     4741 acgtttagcg ccgttgggag aggccggggt cgggagagcg cacctcccgg cccggcctcc
     4801 cggttcgctg ctcccctctc cgccggttcc tccggcctcc cggcccattc cccagggcat
     4861 gcgttgcgca agcggtcgtt agctgaccgg atttcggggc tcgatatgcc ccgattccgc
     4921 cggtagaagt attgacatat cccggaaagc gtgctacaat cgccctagat gttgtgttcg
     4981 atgccgcttg gcgatctaga tatagtagag ggcgcgaaat ttatccgtta cgggaccggt
     5041 gcggcaccgg tctctatcgc gtgactggtg gcgtcaccac ctgccgggga agctatgcgc
     5101 tgtccgtatt gtcataaagg cgaatcgcaa gttctcgata cgcgggagac ccccgatagc
     5161 gacggcatcc gccgccggcg gcgctgtaat aactgcggcc agcgcttcac cacctacgag
     5221 cgggtgcaac cggtgaactt gatgatcgtc aagaaagagg gcctgcgcga ggaatacaac
     5281 cgcggcaaaa tcgaatccgg catccggctc gcctgcacca agcgcccggt gagcgctacg
     5341 accatcgaga atgccgtgaa cagcgtcgaa agcgagcttt tcggcctggg ccagaacgaa
     5401 gtgccagcca aagtcgttgg cgacctggtg cttaaccgcc tgcgcgagat cgatcaagtg
     5461 gcgtatatcc gcttcgcctc ggtgtacctc aactttgccg atctggcgga gatgggccag
     5521 gcgattgagg cgctcttgca gcgcgatacc cgcagcgaac gatctccggc gtgacgggcc
     5581 ggcccctgca ttacatcctg gttagtatgg agtgaagtga agatggcaaa gaccaaaccg
     5641 gcgtcgccgg gcaccacgca tcagcctgat gtcggcgtgc ccctggcgaa gaacgccgtc
     5701 accgtgctcg aacgccgcta cctcgttaag gacgaggccg gcaaggtgat cgagaccccg
     5761 gacgagatgt tccaccgcgt ggcgcgcaac ctggccgagg ccgagggccg ctacggggcc
     5821 gacgtggatc tctgggaaga tcgcttctat cgcctgatgc gctcgctgga gttcctgccc
     5881 aactcgccca cgctgatgaa cgcgggccgg cccctgcaac agctcagcgc gtgcttcgtg
     5941 ctgccggtgg gcgacgacct gggcgagatc ttctccacga tccgcaacca ggccctgatc
     6001 catcagaccg ggggaggcac cgggtttgcg tttagcaggc ttcgacccaa gaacgacatc
     6061 gtcatgacca ccaaaggcaa ggcc
//
LOCUS       DASUYC010000486         4556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_736578,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000486 DASUYC010000000
VERSION     DASUYC010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4556
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736578"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..3496)
                     /locus_tag="VF276_07985"
     CDS             complement(8..3496)
                     /locus_tag="VF276_07985"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6606837.1"
                     /translation="MDTIAPHDGQAAGQDVDQLCPHCQAANRPGARFCTRCGQSLRAG
                     RIPLAAANGERKLVTVMFADISGFTARSEHLDPEEVVTMMNACFVELDKAVSRYEGTI
                     DKFIGDAMMVLFGAPAAHENDAERAVRCALEMQARLAAFSATQPGGDPGTQRPLALHI
                     GINTGLVVAGEIGTPRRRDYTVMGDAVNLASRLESAATAGQILIGAGTHRLVERLFQC
                     RPLPPLALKGKSEPQQAYEVVGVTDTVSARGFERAGVRAPLLGRDRELDLLSNAVQAL
                     ETGQGGLGCIVAEAGLGKSRLTAELRDRVGNRVTWVEARSLAYGAAIPYHALQNAFRQ
                     LLDVPATLAPGEAVARIRKGLAAFGDGADATAPVLWPHVVALLGYPLPDDPAAARLTA
                     MDSAARNGEFSWLLREHWRRVAEVRPTVLVFEDLHWADPATVGLVRDLLPVTREAPLL
                     FLLLYRPENEGPLARLRPLAEGDPRARVLRLQPLTREQSTEMVRVLLDRYHLPPELHA
                     RLLEKAEGNPFYLEEVSRALLDHGAVSTEGGLWVQVQVDEIDVPDSLHGLIATRVDRL
                     SPALKSTLQRAAVIGRHFSTAGLTALVDEPPILPSHLAELEGRELIKPGPATPAAEYA
                     FKHALVQEVAYGSLLKEQRRLLHRVLADHLDPQHTEPGLLAYHYERAEEWPQALRWLT
                     IAAEVAYAQYANATALDLVGRALAAWTEVYPAAAPPAALRTKVALYELLAAVGVTMAD
                     RAAMQQAAEGLRETGAALDDARARVHGYLTATHLNHLESDWDAWEKNIATAHAIAAAS
                     GDQDLISDVYVRQARLYTNRLGPHAAIPYLRRSAAISYLIHDDRELTDSLNNLALAYQ
                     TTGETRRAAWHAAYALKLSLRLGDLRLTAGNYLSVGRIALLRGQLAAGLASMQAGQRD
                     ADRAGQARLQVITRDHVSMAYSAIGLYAQALEAAGEAGALIRRHGLDDLIGFNITTRA
                     DALIGQGRYDDARSALDEMRRRRSTGQLGQWMGPLLVRRSLIDWLDGRPAQARATLIA
                     TRAHLPHLPSDLLACSLWAAAGLGVGDLVDAQARIDQALAWLAQTGNELDAPFTYAVA
                     ARVYAALGRDDLLTAYVAQARAAIRRQWTALAHPRHQAAFAARWARRTAPLGLGDALT
                     PAPAGDQPDMIDSWSQ"
     gene            complement(3657..>4556)
                     /gene="selA"
                     /locus_tag="VF276_07990"
     CDS             complement(3657..>4556)
                     /gene="selA"
                     /locus_tag="VF276_07990"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEX6606838.1"
                     /translation="DIMRQSGATLIEVGTTNRTYLRDYEAALSDRTAGLLRVHTSNFR
                     VTGFTSTVSVADLAALAHRHDLWMADDAGSGALLPTADYGLAPEPLVQDSIAAGADLV
                     IFSGDKLLGGPQAGIIVGRRALVGALRRHPLARALRVDKITYAGLEATLLAYVRGEAT
                     REVPVWAMISADPAGLERRARAWAAQLAGTGVPVAVVPGRTAVGGGTLPGETLPTWLL
                     AVGAQPEQVGPVVPPPGDGEEDGVPPAPSAAALARRLRQGRPAVVARVERDLVLCDPR
                     TVQPDEDAALLAALQTAWGATMP"
BASE COUNT          598 a         1571 c         1724 g          663 t
ORIGIN      
        1 gttcccatca ttgactccaa ctatcaatca tatcgggttg atcaccggcc ggcgcgggcg
       61 tgagcgcatc gcccaggccc agcggggcgg tgcggcgcgc ccagcgggcg gcgaaggcag
      121 cctggtggcg cgggtgcgcc agagcggtcc actggcgccg gatggcggcg cgggcctgcg
      181 cgacataggc ggtgaggagg tcgtcgcggc ccagggcggc atagacccgc gccgccaccg
      241 cgtaggtgaa gggcgcgtcg agttcgttgc ccgtctgggc cagccaggcc agggcctggt
      301 cgatgcgggc ctgggcatca accaggtcgc ccacgcccag gccggcggcg gcccacaggc
      361 tgcaggcgag caggtcgctc ggcaggtggg gcaggtgggc gcgcgtggcg atgagcgtgg
      421 cgcgggcctg ggcgggccgc ccatccagcc agtcgatcag gctgcggcgc accagcagcg
      481 gccccatcca ttgccccagt tgccccgtgc tgcggcggcg gcgcatctcg tcaagggcgc
      541 tgcgggcgtc gtcgtagcgg ccctggccga tgagggcatc ggcgcgggtg gtgatgttga
      601 agccgatcag gtcgtcgagg ccgtgccggc ggatcagtgc cccggcttcg cccgccgcct
      661 ccagcgcctg ggcatagagg ccgatcgccg agtaggccat gctgacgtgg tcgcgggtga
      721 tgacctgcaa gcgcgcttgc ccagcccggt ccgcgtcgcg ctgcccggcc tgcatgctcg
      781 ccagcccggc ggccaactgc ccgcgcaaca gggcgatgcg gcccacgctc aggtagttcc
      841 cggccgtcag gcgcaggtcg cccaggcgca ggctcagctt gagcgcgtag gcggcgtgcc
      901 aggcggcgcg gcgggtctcg ccggtggtct ggtaggccag ggccaggttg ttgagcgagt
      961 cggtcagttc gcggtcgtcg tggatcaggt agctgatcgc ggcactgcgc cgcaggtagg
     1021 ggatggcggc gtgcggcccc aggcggttgg tgtagagccg cgcctgccgc acgtacacgt
     1081 cgctgatcag gtcctggtcg ccggaagcgg cggcaatggc gtgggccgtg gcgatgttct
     1141 tctcccaggc gtcccaatcg ctctccaggt ggttgaggtg ggtcgcggtc aggtagccgt
     1201 ggacgcgggc gcgggcatcg tcgagcgcgg cgccggtctc gcgcagccct tcggcggcct
     1261 gctgcatggc ggcacggtcg gccatcgtca cgcccacggc ggccagcagt tcatagagcg
     1321 cgactttggt ccgcagcgcg gcgggcggcg cagctgccgg gtagacctcg gtccaggcgg
     1381 cgagcgcgcg gcccaccagg tcgagcgcgg tcgcgttggc gtactgggca taggccacct
     1441 cggcggcgat ggtcagccag cgcagggcct gcggccactc ctcggcacgc tcatagtggt
     1501 aggccagcaa gcccggttcg gtgtgctgcg gatcgagatg gtcggccagg acgcggtgca
     1561 acaggcggcg ctgctctttc agcaggctgc cgtaggccac ttcctgaacc agcgcatgct
     1621 tgaacgcata ctcggcggcg ggcgtggccg ggccgggctt gatcagttcg cggccttcga
     1681 gttccgccag gtggctgggc aggataggcg gctcatccac cagcgcggtc aacccggcgg
     1741 tgctgaaatg gcgcccgatg acggcggcgc gctggagcgt gctcttcagc gccgggctca
     1801 ggcggtccac ccgtgtggcg atcaggccat gcaggctgtc cgggacatcg atttcatcga
     1861 cctgcacctg tacccacagg ccgccttccg tgctcacggc gccatgatcg agtagcgcgc
     1921 gggagacctc ttccaggtag aaggggttgc cctcggcctt ttcgagcagg cgggcgtgca
     1981 attcgggcgg caggtggtag cggtcgagca ggacgcgcac catttcggtg ctttgctcgc
     2041 gggtgagcgg ttgcaggcgc agcacgcggg cgcgcggatc gccctcggcc agcgggcgca
     2101 ggcgggccag cgggccctcg ttctcgggcc ggtagagcag caggaagagc agcggcgcct
     2161 cgcgcgtgac cggcagcagg tcgcgcacca ggccgacagt ggccgggtcg gcccagtgca
     2221 ggtcttcaaa cacgagcacc gtggggcgca cctcggccac gcgccgccag tgctcgcgca
     2281 gcagccagga aaactcaccg ttgcgcgcgg cggaatccat ggcggtcagg cgcgcggcgg
     2341 cggggtcgtc gggcagcggg tagcccagca gggccaccac gtgcggccag agcaccggcg
     2401 cggtcgcgtc cgccccgtcg ccgaaggcgg ccaaaccctt gcggatgcgg gccacggctt
     2461 cgcccggcgc cagcgtcgcg ggcacatcga gcaactggcg gaaggcgttt tgcagcgcgt
     2521 gatagggaat ggccgccccg taggccagcg agcgggcttc cacccaggtg acgcggttgc
     2581 ccacgcggtc gcggagttcg gcggtgaggc ggctcttgcc cagcccggcc tcggcgacga
     2641 tgcagcccag cccgccctgc ccggtttcca gcgcctggac ggcgttgctc agcaggtcca
     2701 gctcgcggtc gcggcccagc aacggcgccc gcaccccggc gcgctcgaag ccgcgggcgc
     2761 tcacggtgtc ggtcacgccg accacttcgt aggcttgctg cggctcgctc ttgcccttga
     2821 gtgccagcgg gggcaggggg cggcactgga acagccgctc gaccagccgg tgcgtgcccg
     2881 ccccgatcag gatctgcccg gcggtggccg cgctctccag gcgcgaggcc agattgaccg
     2941 cgtcgcccat gacggtgtaa tcgcggcggc ggggtgtgcc gatctccccg gccacgacca
     3001 ggccggtgtt gatgccgata tgcagcgcca ggggccgctg cgtgccgggg tcgccgccgg
     3061 gctgcgtggc gctgaaggcg gccagccgcg cctgcatctc cagcgcgcag cgcacggccc
     3121 gctcggcgtc gttctcgtgg gccgccgggg cgccgaacag caccatcatc gcgtcgccga
     3181 tgaacttgtc gatggtgcct tcgtagcgcg acaccgcctt atccagctcg acaaagcagg
     3241 cgttcatcat ggtcacgact tcttcgggat cgagatgttc gctgcgcgcc gtgaacccgc
     3301 tgatgtcggc gaacatgacc gtgaccagct tgcgctcgcc gttcgcggcg gccaggggaa
     3361 tgcgcccggc gcgcaggctc tggccgcagc gcgtgcaaaa ccgcgcaccc ggccgattgg
     3421 ccgcctggca atgagggcag agctgatcca cgtcctgccc ggcggcctgg ccgtcatgcg
     3481 gggcgattgt gtccatgtcc cggtgtctcc tctaacaaat gctgaagcgt tccggcgtgc
     3541 tacttgcctt catccggcag tgctgattgt tctcgcatgc taccgtgggc caaactccgc
     3601 ggtaggggtg cggcggtgcg ggccgcatgt agttcggcgg gccgggggcg gcccaatcag
     3661 ggcattgttg ccccccaggc ggtttgcagc gcggcgagca gcgccgcatc ttcgtccggc
     3721 tggacggtgc gcgggtcgca caagacgagg tctcgctcga cgcgcgcgac aacggcggga
     3781 cggccctggc gcaagcgccg ggccagggcc gcggcggatg gcgccggcgg caccccatct
     3841 tcctcgccgt cgccgggcgg gggcacgact ggcccgacct gctcgggctg cgcgccgacg
     3901 gccagtagcc aggtcggcaa cgtctcgccc ggcaaggtgc cgccgcccac ggcggtgcgg
     3961 ccagggacga cggcgacggg caccccggtc cccgcaagct gcgccgccca ggcgcgcgcc
     4021 cgcctttcga gccccgccgg gtccgcgctg atcatggccc acaccggcac ctcgcgggtc
     4081 gcctcgccgc gcacataggc cagcagggtg gcttccagcc cggcgtaggt gatcttgtcg
     4141 acgcgcaggg cgcgggccag cgggtgccgg cgcaacgccc cgaccagagc gcggcggccc
     4201 acgataatcc cggcctgcgg gccgcccagc aacttgtcgc cgctgaagat gaccaggtcg
     4261 gcgcccgccg cgatgctgtc ctggaccagc ggttccggcg ccaggccgta gtccgccgtg
     4321 ggcagcaggg cgccgctgcc ggcgtcgtcg gccatccaga gatcgtggcg atgggccagc
     4381 gcggccaggt cggccacgct caccgtgctg gtgaagccgg tcacgcggaa gttgctggtg
     4441 tggacgcgca gcaggccggc ggtccggtcg ctcagcgcgg cctcgtagtc gcgcaggtag
     4501 gtgcggttgg tggtacccac ttcgatcagg gtggccccgc tctggcgcat gatgtc
//
LOCUS       DASUYC010000487         2255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_76994,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000487 DASUYC010000000
VERSION     DASUYC010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76994"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VF276_07995"
     CDS             complement(<1..576)
                     /locus_tag="VF276_07995"
                     /inference="COORDINATES: protein motif:HMM:NF014135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX6606839.1"
                     /translation="MTPAFIAGVIVFIFTLVLIMARPRGLSEAWAALGGAAAMLLLGL
                     VSLAETRDLLVANLNVFGFFLGLMAIAALADSAGVFAWLAGIAARTARGSARRLFVNV
                     FLVGVLITVFLSNDATALILTPLVYTLVTRLRLPALPYMFACTFIADTASFVLPVSNP
                     INIIVLQAFPRDLGTYLGHLLPAAVLVILANI"
     gene            complement(592..1176)
                     /locus_tag="VF276_08000"
     CDS             complement(592..1176)
                     /locus_tag="VF276_08000"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX6606840.1"
                     /translation="MSDDTGARDQRARLGPKLGSGDEGYTDLLGPDRVPKYDLRVQSY
                     GELDEASSALGLARALAQQPRVKEIIRQVQHDLYLMMAEVASPPAQVVKLPYRITAEQ
                     VAWLETTITALEDEVEMPRQFILPGAGPGSAALDVARTVVRRAERSTVHLSSTGAMPA
                     GQVLRYLNRCSSLLFVLARYEEAVSGIPYDIAKR"
     gene            complement(1378..1860)
                     /gene="mscL"
                     /locus_tag="VF276_08005"
     CDS             complement(1378..1860)
                     /gene="mscL"
                     /locus_tag="VF276_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003335255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEX6606841.1"
                     /translation="MAAGEKPKSLREFLMQGNVIDLATAVIIGAAFGAVVTSLVNDII
                     MPPIGLLLGGVDFSNLFINLSGTSYPSVAAAKAAGAPTINYGLFINAVISFLIIGAVI
                     YFLVRALTRLTPETKAPTPEVETKVCPYCISDVPLKATRCPYCTSDLTEGARSALPAR
                     "
     gene            complement(2021..>2255)
                     /locus_tag="VF276_08010"
     CDS             complement(2021..>2255)
                     /locus_tag="VF276_08010"
                     /inference="COORDINATES: protein motif:HMM:NF025589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HEX6606842.1"
                     /translation="EWVNRADLMRLKGVGTEMANLLEECGVDSCKELQHRVAANLQAK
                     LKATNDEKHITHHAPTVAQVEEWIHEAAQLSAQ"
BASE COUNT          381 a          714 c          795 g          365 t
ORIGIN      
        1 gatgttcgcc aggatgacca gcaccgcggc gggcagcagg tggcccaggt aggtgcccag
       61 gtcgcgcggg aaggcttgca gcacgatgat attgatcggg ttgctgaccg gcaggacgaa
      121 cgacgcggtg tccgcgataa aggtacaggc gaacatataa ggcagggccg gcaggcgcag
      181 gcgcgtgacc agggtataga ccagcggcgt gaggatcagc gcggtggcgt cgttggagag
      241 gaacacggtg atcagcacgc cgaccaggaa cacattgacg aacaggcgcc gcgcgctgcc
      301 ccgcgccgtg cgcgcagcga tcccggccag ccaggcgaag acgcccgcgc tgtcggccag
      361 ggcggcaatc gccatcaggc ccaggaagaa gccgaagacg ttgaggttgg cgaccagcag
      421 atcgcgcgtc tcggccagcg agaccagccc cagcagcagc atggccgccg ccccgccgag
      481 cgcggcccag gcttcggaca ggccgcgggg tcgggccatg atgagcacca gggtgaagat
      541 gaacacgatc accccggcga tgaaggccgg cgtcatgggt actcccggcg gttagcgttt
      601 ggcgatgtcg tagggaatgc cggagaccgc ttcttcgtag cgggccagca cgaacagcag
      661 cgacgagcag cggttgaggt agcgcagcac ctgcccggcg ggcatcgccc cggtgctgct
      721 caggtggacc gtggatcgct cggcccggcg cacgacagtt cgcgccacgt cgagggcggc
      781 ggatccaggg ccggcccccg gcaggataaa ctggcgcggc atctcgacct cgtcttccag
      841 cgccgtgatc gtcgtctcca accaggccac ctgctcggcg gtgatgcggt agggcagctt
      901 gaccacctgg gcgggcgggg aagcgacctc ggccatcatc aggtagagat cgtgctgcac
      961 ctggcggatg atctccttga cgcgcggctg ctgggccagg gcgcgggcca ggccgagcgc
     1021 actgctggcc tcgtcgagtt cgccgtagct ttgcacgcgc aggtcatact tcggcacccg
     1081 gtcggggccg agcaggtcgg tatagccctc gtcgccggag ccgagtttgg gccccagccg
     1141 ggcgcgctgg tctcgcgcgc cggtgtcgtc gctcacagtg gttgcctccc agccgaaggt
     1201 tgcgggcgca aaaatgcgca tggtccgtgg tcttcaccgt accatgcgcg caggcgggtg
     1261 tcaaggcggg caacggggag gccgatcccc gcccccggag cgggaaccat gtcgcggcgc
     1321 acggttcccg ccccccggag gggaaccgcg tcgcgattcg cggttcccct ggttcgatta
     1381 gcgtgcgggc agcgcggacc gcgccccttc ggtcaggtcc gacgtgcaat acgggcagcg
     1441 ggtagccttc agcggcacat cggagatgca gtacgggcag actttcgtct cgacttcggg
     1501 cgtgggggcc ttggtttccg gcgtcagccg cgtgagcgcg cggacgagga aatagatcac
     1561 cgcaccgata atcaggaagc tgattacggc gttgatgaat agcccgtagt tgatcgtcgg
     1621 cgcccccgcc gccttggccg cggccacgct gggatacgat gtgccggaga gattgatgaa
     1681 caggttggag aagtcgactc cacccagcag caacccgatc gggggcatga tgatgtcgtt
     1741 gacgagcgac gtgaccaccg cgccgaaggc ggcgccgatg atcacggccg tcgccaggtc
     1801 gatgacgttg ccctgcatca ggaattcgcg gagcgatttc ggtttttcgc ctgcagccat
     1861 aattgatctc cttgataaaa cggattcttc gttgtgggtg gcccctgggc agtccgggtg
     1921 ggccaccaga ccggttgcaa accggcccct gcctcgctaa atctgtgcta tgcgcgggcc
     1981 ggttgcaaat cagccccgac gccgccaaat cggcgcgccg ttactgggcc gataactggg
     2041 ccgcctcgtg gatccactcc tccacctggg ccaccgtggg cgcatgatgg gtgatgtgct
     2101 tctcgtcgtt ggtggccttg agcttggcct ggaggttcgc ggcgacgcga tgctgcagct
     2161 ctttgcagga gtcgacgccg cattcttcca gcaggttcgc catctcggtg cccacgccct
     2221 tgaggcgcat caggtcggcc cggttcaccc actcg
//
LOCUS       DASUYC010000488         3498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_330546,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000488 DASUYC010000000
VERSION     DASUYC010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330546"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="VF276_08015"
     CDS             complement(<1..906)
                     /locus_tag="VF276_08015"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HEX6606843.1"
                     /translation="MSHHYSGPDFSFPQGDARLDLCDLYAFLKPGDPSKTILIMDVHP
                     SVGVNPPGPTTPEPFAPGAIYELKIDTDADVVADIAYRVLFAQGANGAQTATVRRAEG
                     ADAAGTGDSGETLIADAPVSMGRDAQVTTAGDYRFFAGWRSDPFFFDVNGAVNNLQFT
                     GDDFFADKNVCSIALELPNAALGPRAMALWHRTLAPAVDGAGWMQADRGARGSQSVFF
                     CPGAEKAAYLGGDPLDDARFVDSFAHVLQHSGGYRAQEARQVAQTLLPDIIPYDPAHP
                     ALYSQNGRTLTDDVSDQFLAVFTNGK"
     gene            1188..2600
                     /locus_tag="VF276_08020"
     CDS             1188..2600
                     /locus_tag="VF276_08020"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX6606844.1"
                     /translation="MTTPNILLFLTDDHGAWATGCYGNSEVQSPTLDQLAREGARFAH
                     AFTPSPVCSPARACLLTGRTPSQVGIHDWIQDSEPEFGDYDWLRDEVTLPELLHDAGY
                     YCGLSGKWHLGRAQETPRGFDWYFGLPGWQGDHIETYTYVLNGQPVTLTGNKTGFITD
                     HALRFLDTTPADRPFFLQVGYIATHSPYANQEPDLVARYRDATFRDIPPYEPHPWRQN
                     EGFPTRTGWTEADLRSRYASYYAAVTDMDRHAARLLDRLRAQGRLDSTLVIYTSDHGL
                     ALGHHGFWGKGNSTRPLNMYEISIQVPLILRLPGEIPADYVAPRCVDHYDTFQAICDW
                     AGVDPARAAPPNHYPGRSYRPLAQPTPPPDWSDTICGEYGDLRMIRTPHYKLVRRYPS
                     GPDDLFDLRADPGERTNLAADPAHAATVRALTADLDAFYARYSDPAHSGLRVKDLRQH
                     NRHVEAWRDGIREARGLQRY"
     gene            complement(2638..>3498)
                     /locus_tag="VF276_08025"
     CDS             complement(2638..>3498)
                     /locus_tag="VF276_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606845.1"
                     /translation="REKVFLSQVVHSGEITDMELPAHIEAGVWADLALELLAQQAASA
                     ELDETDVAENPPNPQAEQAQREKWEAYEEQRANEIFAKMQATRRRDEEHFSRLATYYL
                     HRTGLERIAYHRSLHLVLRRFASQRRLSISEVAALEQILRRLGHRQAAVAAACYELLA
                     AHERNRHYLPTACHYWREAELPERVLEIAVPSDGDSAALQTMLLNTRSAALADMECWS
                     EARQTAYRASRTQPSSKHSFNVLGRIAYATGAADLAQHYFTRAQHGSTDRPPASSAKG
                     PLPHRNEDLE"
BASE COUNT          591 a         1208 c         1131 g          568 t
ORIGIN      
        1 tttgccgttg gtgaacacgg ccaggaactg gtcgctcacg tcgtcggtga gcgtgcgccc
       61 gttttgcgag tagagcgcgg ggtgggcggg gtcgtaggga ataatatcgg gcaacagcgt
      121 ctgcgccacc tgccgcgctt cctgcgcgcg atagccgccg ctgtgctgga gcacgtgggc
      181 gaagctgtcc acgaagcggg catcgtccag cgggtcgccg ccgagatagg cggccttttc
      241 ggcgccgggg cagaagaaga ccgactgcga tccgcgggcg ccgcgatcgg cctgcatcca
      301 gcccgccccg tccaccgccg gggccagcgt gcggtgccag agggccatgg cgcgggggcc
      361 cagcgcggcg ttgggcagtt ccagcgcgat gctgcacacg ttcttgtcgg cgaagaagtc
      421 gtccccggtg aattgcaggt tgttcaccgc accgttcacg tcgaagaaga aggggtcgct
      481 gcgccagccc gcaaagaagc ggtagtcccc ggcggtcgtc acctgggcgt cgcggcccat
      541 cgatacgggc gcatcggcga taagcgtctc cccgctgtcg ccggtcccgg cggcgtccgc
      601 cccttcggcc cggcgcacgg tcgcggtttg cgccccgttg gcgccttgcg cgaacagcac
      661 gcggtaggcg atgtcggcga ccacgtcggc atcggtgtcg atcttaagct cgtagatggc
      721 gccgggcgcg aacggctcgg gggtcgtcgg gccgggcggg ttcacgccca ccgacggatg
      781 cacgtccatg atcaggatgg tcttgctcgg gtcgccgggc ttgaggaagg cgtacaggtc
      841 gcagaggtcg aggcgggcgt cgccctgcgg gaacgagaaa tcgggtcccg aataatggtg
      901 ggacatgggg cttcctttct gcggtagcgg cggggccgga ggctgccgtt gcggtctccg
      961 cacaggcgcg cgctcggttg gcagcagatt ccatgccccg gcggggcggc acatacgaga
     1021 tttcaccgcg aaggaacgaa gataacgaag acgattaata gaaaagatct tcgttacctt
     1081 cgtttcttcg cggtgaagaa acgggcggac acggcgcgat aacccgcggc ggggtgcgcc
     1141 cgttctacag ggtagcgaat ggcccggcac agacccagga ggcgcccatg accaccccca
     1201 acatcctgct cttcctgacc gacgaccacg gcgcgtgggc caccggctgt tacggcaact
     1261 ccgaggtgca atcgcccacg ctggaccagc ttgcgcgcga gggggcgcgc ttcgcccatg
     1321 ccttcacgcc cagcccggtc tgctcgcccg cgcgggcctg cctgctcacc gggcgcaccc
     1381 cgtcacaggt gggcatccac gactggatcc aggactccga acccgagttc ggcgactacg
     1441 actggctgcg cgacgaagtg accctgccgg aactgttgca cgatgcgggc tactactgcg
     1501 gcctcagcgg caagtggcac ctgggccggg cgcaggagac accgcgcggc ttcgactggt
     1561 atttcggcct gccgggctgg cagggcgacc acatcgagac ctacacctac gtgctcaacg
     1621 gccaaccggt gacgctgacc ggcaacaaga ccggcttcat caccgaccac gcgctgcgct
     1681 tcctcgacac gacgcccgcg gaccgcccgt tcttcctgca ggtgggctat atcgccacgc
     1741 actcgcccta cgccaaccag gagccggacc tggtggcccg ctaccgcgac gcgactttcc
     1801 gcgacatccc gccctacgag ccccacccct ggcggcaaaa cgagggcttt cccacgcgca
     1861 cgggctggac cgaggccgac ctgcgtagcc gctacgccag ctactacgcc gccgtgaccg
     1921 acatggaccg ccacgccgcc cggctgctcg accggctgcg cgcgcagggg cggctcgaca
     1981 gcacgctggt catctacacg tcggaccacg ggctggcgct cggccatcac ggcttctggg
     2041 gcaagggcaa cagcacccgc ccgctcaaca tgtacgagat ctcgatccag gtgccgctga
     2101 tcctgcgcct gcccggcgaa atccccgccg actacgtggc gccgcgctgc gtggaccact
     2161 acgacacctt ccaggccatc tgcgactggg ccggggtgga cccggcgcgc gccgccccgc
     2221 ccaaccacta ccccggtcgc agctaccgcc cgctggccca gcccacgccc ccgcccgact
     2281 ggagcgacac catctgcggc gaatacggcg acctgcgcat gatccgcacg ccgcactaca
     2341 agctggtgcg gcgctatccg tccggcccgg acgacctgtt cgacctgcgc gccgaccccg
     2401 gcgagcgcac caacctggcc gccgaccccg cccacgccgc cacggtgcgc gccctcacgg
     2461 cggacctcga cgccttctac gcccgctaca gcgacccggc ccacagcggc ctgcgcgtca
     2521 aagacctgcg ccaacacaac cgccacgtcg aagcctggcg cgacggcatc cgcgaagccc
     2581 gcggcttgca gcggtactga cactgctaac cgtagtgcat tgccccgttt atcaccgcta
     2641 ttctagatcc tcatttcggt gaggaagagg accttttgct gaggatgcgg gaggccgatc
     2701 tgtcgaaccg tgttgcgccc gcgtaaaata gtgctgggca aggtcagcgg cgcccgttgc
     2761 gtaagcaata cggcccagga cgttgaagga gtgcttactg cttggctgcg tcctggaggc
     2821 gcggtaggct gtctggcgag cctccgacca gcattccatg tcagccagcg ctgcgctgcg
     2881 tgtattcagc agcatggtct gcaaggccgc ggaatcaccg tcggacggga cagcgatctc
     2941 tagcacgcgc tccggcagct cggcttcccg ccaataatga caagctgtgg gcaggtagtg
     3001 gcggttacgt tcatgagcag ctagcagttc atagcaagcg gcggcaaccg ccgcctggcg
     3061 gtgccccaag cgccttagga tctgttccag cgccgcgacc tcagaaatgc tgagacgccg
     3121 ctgagaggcg aaccgccgca acacgaggtg gagtgaccgg tgataagcga tccgttctag
     3181 tccggtgcgg tggagataat aagtcgccag ccgagagaag tgttcctcat ctcgccggcg
     3241 cgtggcctgc attttcgcaa aaatctcgtt ggcccgttgc tcctcgtaag cttcccattt
     3301 ttcccgctgg gcctgctcgg cctggggatt gggtgggttt tccgccacat ctgtctcatc
     3361 caattctgca gatgccgctt gttgggccag cagttccaag gccaggtcgg cccacacacc
     3421 ggcctcaata tgagccggca actccatatc ggtgatttcg ccactgtgga ctacttgaga
     3481 taggaacacc ttctcgcg
//
LOCUS       DASUYC010000489         3773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1903420, whole genome shotgun sequence.
ACCESSION   DASUYC010000489 DASUYC010000000
VERSION     DASUYC010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1903420"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="VF276_08030"
     CDS             complement(<1..676)
                     /locus_tag="VF276_08030"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6606846.1"
                     /translation="MFCTHLTLAEFRNYAALDCDLPEGISVIQGANGSGKSSLLEALY
                     LLATARSPRASVDAELVNTEAAADLGVPPFARVAAEVTRRTDAVALEVIIAREDSRSL
                     TVPPGDSPDLAAAAPASPVARKRIKINDIPRRAVDLVGQINVVLFTPEDVDLVTGEPS
                     LRRRYMDITISQVDHRYIRTLSQYNKVVLQRNALLRQMREQGRNPLSPSVAEELRYWD
                     EELARAG"
     gene            complement(690..1649)
                     /locus_tag="VF276_08035"
     CDS             complement(690..1649)
                     /locus_tag="VF276_08035"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX6606847.1"
                     /translation="MLTYYSLRIGLLLAAIIPSRLAYLICSIVGNVAFYTNGSARRAV
                     QDNLRHVFGPTMSRRKLNRVARFVFRNTVKNYYELLRLPEMSAGDLEKRVTVIGIENI
                     DAALKAGRGAIIFSGHIGNFNLVAQVAGARGYPTNIVAEQMEPPKLHELVNGLRSRFG
                     LKMIPLGSSAVRGIYHALRANEILGLAADRDLTANGIPVEFFGETTELPSGLAALSLR
                     LNAPLLPVHVVRKANDSSVVTIYPPLELARTGNRETDVQAGTQQIAGVLEGMIRKTPE
                     QWVVLQPVWPDDAPATSAPAPNGASNEAPPPAEDLPDRQLTRA"
     gene            complement(1653..2825)
                     /locus_tag="VF276_08040"
     CDS             complement(1653..2825)
                     /locus_tag="VF276_08040"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX6606848.1"
                     /translation="MVESVQQQHPAGPLARCVARVSPGGKYWGFRVLSRLASVVPIRI
                     SYKVAVLIGDFIYYTWHEHSANAVGNMRRVLGPRAGERAARRAARASFRNYAKTLADF
                     IRFPHMADGEVEARVREHYGADRLDELRAKGKGVIAVSGHLGNWDFAGAYLGRRGLPL
                     YAIADRFEPPKLDKLVIETRLRNGIHVITMEASAMRTIFEALRRNEVVMLLIDRPMPG
                     EGVPVQFFGETAWLPSGPAAIALKTGAPLVIGYCVRMRGDVCFQGGIEAPIEYKHLIT
                     GNKQADIQAITQAIACAMEALIRRTPDQWYMFRPMWPRPETAAERRRGRRQARRVALR
                     RRARQLRHARPSITRLRARIRGAAVPSADLSGVSLGISVDPPEMEEWVAGQGPQER"
     gene            complement(2899..>3773)
                     /locus_tag="VF276_08045"
     CDS             complement(2899..>3773)
                     /locus_tag="VF276_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEX6606849.1"
                     /translation="EKIVKAPGETADIVQILKDTHTDVVVSYLPVGSEQATKWYVEQV
                     IEAGCGFVNCIPVFIAREDYWNNRFKKAGLPIVGDDIKSQVGATITHRVLTRLFAERG
                     VKIDRTYQLNFGGNTDFLNMLERERLESKKISKTNAVTSQVGYPMPARDVHVGPSDYV
                     PWLDDRKWCYIRMEGTTFGNVPLNMELKLEVWDSPNSAGVVIDAVRCCKLGLDNGLSG
                     TLEGPAAYFMKSPPVQHPDDLAHDMVETFIAEQVQAGAERRARQDSAKTKSSPEPIPA
                     ELHPVTPASNGHSN"
BASE COUNT          564 a         1261 c         1286 g          662 t
ORIGIN      
        1 ccccggcgcg cgccagttcc tcgtcccagt agcgcaactc ctcggccacg ctgggcgaga
       61 gcgggttgcg gccctgctcg cgcatctgcc gcagcagggc gttgcgctgc aagacgacct
      121 tgttgtactg cgacagcgtg cggatgtagc ggtgatccac ctgggagatg gtgatgtcca
      181 tatagcgccg gcgcagcgac ggctcgcccg tcaccaggtc cacgtcctcc ggcgtaaaca
      241 gcaccacgtt gatctgcccg accaggtcca cggcgcggcg ggggatgtcg ttgatcttga
      301 tgcgcttgcg ggccacgggg ctggcgggcg cggcggcggc caggtcggga ctgtcgccgg
      361 gcgggacggt caggctgcgg ctgtcttccc tggcgatgat cacctccagg gccacggcat
      421 cggtccggcg cgtgacctcg gcggcgacgc gggcgaaggg cggcacaccg aggtcggcgg
      481 cggcttcggt attcaccagt tccgcgtcca ccgaggcgcg tggcgaccgc gccgtcgcca
      541 gcaggtagag ggcttcgagc aggctacttt tgccgctgcc gttcgcgccc tggatcacgc
      601 tgatgccttc cggcaggtca cagtccagcg cggcgtaatt gcgaaactcg gcaagcgtga
      661 gatgggtgca gaacatgctg cggctttcgc taggcgcggg tcaactggcg atccggcagg
      721 tcctcggcgg gcggcggggc ttcgttcgat gccccgttag gggcaggggc ggatgtggcc
      781 ggggcgtcgt cgggccagac cggttgcaac accacccatt gctcgggcgt cttgcggatc
      841 atcccttcga gcaccccggc gatctgctgg gtcccggcct gcacatcggt ctcgcggttg
      901 ccggtgcgcg ccagttcgag aggcgggtaa atcgtcacga cggatgagtc gttggccttg
      961 cgtaccacat ggaccggtag caagggcgcg ttcaggcgca acgacagcgc ggccaggccc
     1021 gacggcaatt cggtggtctc gccgaaaaac tcgaccggga tgccgttggc cgtcaggtcg
     1081 cggtcggcgg ccaggcccag gatttcgttg gcccgcaagg cgtgatagat gccgcgcacg
     1141 gcgctcgacc ccagcgggat catcttcagc ccgaagcgcg agcgcaggcc gttgaccagt
     1201 tcgtgcagct tgggcggctc catctgctcg gcgacgatgt tggtcggata gccgcgcgcc
     1261 ccggccacct gcgccaccag gttgaagttg ccgatatggc cggagaagat aatcgccccc
     1321 cgccccgctt tcagggccgc atcgatattc tcgatgccga tcaccgtcac ccgcttttcc
     1381 aggtcgccgg cgctcatttc gggcagccgc agcagttcgt agtagttctt gacggtgttg
     1441 cggaacacga accgtgccac ccggttaagc ttgcgccggc tcatggtggg gccgaacaca
     1501 tggcgcagat tgtcctgcac cgcgcgccgg gccgagccgt tcgtgtagaa cgccacgttg
     1561 cccacgatcg agcagatcag gtaggccagc cgggagggga tgatcgcggc cagcaacaga
     1621 ccgatgcgca agctgtagta cgtcagcata ggttatcgct cctgcggccc ctggcccgcg
     1681 acccattcct ccatttcggg cgggtcgacg ctgatgccca gcgacacgcc gctcaaatcg
     1741 gcgctgggca ccgccgcgcc gcggatgcgc gcccgcagcc gggtgatgct gggccgcgcg
     1801 tggcgcagct gccgggcgcg ccgccgcagg gccacccgcc gggcctgccg tcgcccccgc
     1861 cggcgctcgg cggccgtctc ggggcgcggc cacatcgggc ggaacatata ccactggtcc
     1921 ggcgtgcgcc ggatcagcgc ttccatagcg cacgcgatgg cctgcgtgat ggcctggata
     1981 tcggcctgct tgttcccggt gatcaggtgc ttgtactcaa tcggcgcctc gatgccgccc
     2041 tggaagcaca cgtcgccgcg catgcgcaca caatagccga tgaccagcgg cgccccggtc
     2101 ttgagcgcga tcgccgcggg gccgctgggc agccaggcgg tttcgccgaa gaattgcacc
     2161 ggcacgcctt cgcccggcat gggccggtcg atcagcagca tcacgacctc gttgcgccgc
     2221 agcgcctcga agatggtgcg catggcgctg gcttccatcg tgatcacgtg gatgccgttg
     2281 cgcagccgcg tctcgatgac cagcttatcg agcttgggcg gctcgaagcg gtcggcgatc
     2341 gcgtagagcg gcagcccgcg ccggcccaga taggcgcccg cgaaatccca gttgcccagg
     2401 tgcccgctga ccgcgatgac gcccttgcct ttggcccgca actcgtccag gcgatcggcg
     2461 ccgtaatgtt cccgcacccg tgcttccacc tcgccgtcgg ccatgtgggg gaagcggatg
     2521 aaatcggcca gggtcttggc atagttgcgg aagctggcgc gcgccgcgcg ccgcgcggcc
     2581 cgttcgccgg cccgcggccc cagcacgcgg cgcatattgc ccacggcatt ggcgctgtgc
     2641 tcgtgccagg tgtagtaaat aaagtcgccg atgagcacgg ccactttata cgagatccgg
     2701 atcgggacca cgctcgccag ccggctcagg acgcgaaagc cccagtactt gccgccgggt
     2761 gagacgcggg ccacgcagcg ggcaaggggc cccgccgggt gctgttgttg tacactctcc
     2821 accatgtacc atcaccgata gggggcgcgg gctgcaccca aactcggcct atgtgccgcc
     2881 cgccgccccc ggctaagctt agtttgaatg accgttgctg gccggcgtca cgggatggag
     2941 ttccgccggg atcggttcgg gactgctctt ggttttcgcc gagtcctgcc gggcacggcg
     3001 ctcggcgccc gcctggactt gctcggcgat aaaggtctcg accatgtcat gcgccagatc
     3061 gtccgggtgc tgcaccggcg gcgacttcat gaagtaggcc gccgggcctt cgagcgtgcc
     3121 gctgaggccg ttgtcgaggc ccagcttgca gcaacgcacc gcgtcgatga ccacgcccgc
     3181 cgagttcggc gagtcccaga cttcgagttt cagttccatg ttcagcggca cgttgccgaa
     3241 ggtcgtgcct tccatgcgga tatagcacca cttgcggtcg tccagccagg gcacgtaatc
     3301 gctggggccg acatgcacgt cgcgcgctgg catcgggtag ccgacctgcg acgtgacggc
     3361 gttcgtcttg ctgatcttct tcgactccag ccgttcgcgc tccagcatat tgaggaagtc
     3421 ggtattgccg ccgaagttca gttggtaggt gcggtcgatc ttgacgccgc gttcggcgaa
     3481 cagtcgcgtc agcacacgat gggtgatcgt ggcgccgacc tgcgacttga tgtcgtcgcc
     3541 gacgatcggg aggccggcct tcttgaagcg gttgttccag tagtcctcgc gggcgatgaa
     3601 caccgggatg cagttgacga agccgcagcc ggcctcgatg acctgctcga cgtaccactt
     3661 cgtcgcctgc tcggagccga ccggcaggta cgacacgacg acgtccgtgt gcgtgtcctt
     3721 gaggatctgc acgatgtcgg ccgtctcgcc cggggccttg acgatcttct cct
//
LOCUS       DASUYC010000490         3926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1390347, whole genome shotgun sequence.
ACCESSION   DASUYC010000490 DASUYC010000000
VERSION     DASUYC010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1390347"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VF276_08050"
     CDS             <1..358
                     /locus_tag="VF276_08050"
                     /inference="COORDINATES: protein motif:HMM:NF024298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX6606850.1"
                     /translation="SSGDVNSIRWSPDGKRLAAGFADGNLRIWDMMQNQTLDTIVYRD
                     EVKDLAWSPDSRQIAFGGWSLSDNVLVKVWSLSSKQTTLTFPHQDNVNTIAWSPDGNY
                     IASGTQDGVVWLWNVK"
     gene            complement(467..1882)
                     /locus_tag="VF276_08055"
     CDS             complement(467..1882)
                     /locus_tag="VF276_08055"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX6606851.1"
                     /translation="MLRRLIVLFVIVPALLAGARPAAASEAVPRSPLPLPPAASPLLS
                     GGFAQFWQDHNGALLLGAPLTGELQEGGLTVQYLERARLEWHPDYPPGRQITLGRLGA
                     EAVQGRAFPTIAAFPSNPGHLYFAATGHSIQGDMLRFWQANGGLAIFGYPLSEELTED
                     GRTVQYFERARLEHHPELATTGYRVLPTPLGALAVARRATTAAVVFEPPMVQEGHTVL
                     IKVLPAPGTRLVGGTLGGNTLSFTCCLPLSLPGGAQTLPWAVGGAESYQSPDTLSLRL
                     TLQDADGATTQIERALPVQRFPYPTLRQQYNGPRINPAVRDNEHAIIDAVFAGRDLGP
                     QWQGPFVAPLHRSLVVNAPFGQRRAYNAEPPYEVHTGIDFDAATGTPVYAPAPGRVVF
                     ARPLRLRGNTVIVDHGGGVFTLYAHLSAFRATVGQRVQTGDLLALVGSTGNALGPHLH
                     WEVHAAGPAVQPEQWLARSFP"
     gene            2103..3563
                     /gene="glmU"
                     /locus_tag="VF276_08060"
     CDS             2103..3563
                     /gene="glmU"
                     /locus_tag="VF276_08060"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEX6606852.1"
                     /translation="MTTEPATEDPTASPAPRLAAVVLAAGMGTRMRSRLPKVLHRIAG
                     VPLVEHVARAVAPLAPARTVLVVGHGGEQVQAALGPGYEYVTQAQQLGTGHAVLQARA
                     AVEGQAGTVLVLYGDSPLLRSTTLADLVSLHHSRPGVRATILTCIAEDPTGYGRIIRD
                     ATGQVFDIVEERVATPEQRAIKETNSGIYCFDSAWLWPRLAELPIHPGAGEYYLTDMI
                     AVAIREQSGSVQTFQVSGMEEVAGINNRVQLAEADLVIRNRIRTRWMLEGVTMIDPAT
                     TYIDVDVTLGQDTILYPGCYLQGQTRVGADCRLGPQARLVDAVVGDGCVVGTSLLEEC
                     VVEAGVDIGSFNHLRPGAYLSSGVHLGNFAEVKNSRLGRNVSQGHFSYIGDAEVGDNT
                     NIGAGTITANFNNGVKSRTQIGPDVFIGSDSVLIAPVEVGAGAQTGAGAVVNKDVPPG
                     ALVVGVPARVIRWLNGGPPERGGPATPPREGSDPPA"
     gene            3560..>3926
                     /locus_tag="VF276_08065"
     CDS             3560..>3926
                     /locus_tag="VF276_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase-like
                     domain-containing protein"
                     /protein_id="HEX6606853.1"
                     /translation="MSRALQVFSGSANRPLAEAICRCLGVRLGKAIVGRYRNGETRVR
                     VDESVRGDDVYVIQPTSNPVNENLMELLIMLDALRRSSAARVTAVIPYYGYAKQEKKT
                     APREPITAKLVANLLVEAGA"
BASE COUNT          675 a         1284 c         1349 g          618 t
ORIGIN      
        1 gtcttccggt gatgtgaaca gtattagatg gtctcctgac ggcaaacgtc tagcagcagg
       61 ttttgcagat gggaatctgc gtatatggga tatgatgcaa aaccaaacgc tagacacaat
      121 tgtctacaga gatgaagtta aagatcttgc ctggtctcct gatagccggc aaatcgcatt
      181 tggaggatgg agtttaagcg ataatgtact agtgaaggtt tggtcattat ccagcaagca
      241 aaccacttta acattccctc atcaagataa cgtaaataca atcgcttggt ctccggacgg
      301 caattacata gcatcaggaa cgcaagacgg ggtagtatgg ctatggaacg taaagtgaaa
      361 aacaaaggaa agtgtctgct cgccatgccg ctttctgtgt ttcgcgacct gggatgatgg
      421 ctataacagc actggaccga gatgcacacg cgagctacag ggttgctcac gggaagctgc
      481 gcgccagcca ctgctccggc tgcacggcgg ggccggcggc gtggacctcc cagtgcaggt
      541 gcggccccag cgcgttgccc gtgctgccga ccagggccag cagatcgccc gtctgcacgc
      601 gctggcccac ggtggcgcgg aaggccgaga ggtgcgcgta gagcgtgaac acgcccccgc
      661 cgtggtccac gatgacggtg ttgccgcgca gccgcagcgg gcgcgcgaac accacccgcc
      721 ccggcgccgg ggcatagacc ggcgtcccgg tggccgcgtc gaagtcgatg cccgtatgta
      781 cctcgtaggg cggttcggca ttatacgcgc ggcgctggcc gaagggcgcg ttgaccacca
      841 ggctgcggtg caagggcgcg acgaacgggc cctgccattg cggccccagg tcgcgcccgg
      901 cgaacacggc gtcgataatg gcgtgctcgt tatcgcgcac ggcggggttg atgcgcgggc
      961 cgttgtactg ctggcgcagg gtggggtagg ggaagcgctg caccggcagc gcgcgctcga
     1021 tctgcgtcgt ggcgccgtcc gcgtcttgca aggtcaggcg caagctcagc gtgtcgggcg
     1081 attggtacga ttctgcgccg cccaccgccc agggcagcgt ctgcgcgccg cccggcaggc
     1141 tcagcggcag gcagcaagtg aacgacagcg tgttgccgcc cagcgtgccg cccaccaggc
     1201 gcgtgccggg cgcgggcagc accttgatca gcaccgtgtg gccctcttgc accatcggcg
     1261 gctcgaacac cacggcggcg gttgttgccc gccgggccac ggccagggcg ccgagcggcg
     1321 tgggcagcac ccggtagccg gtcgtcgcca gttcggggtg gtgttccagg cgggcacgct
     1381 cgaagtattg caccgtgcgc ccgtcctcgg tcagctcctc ggagagcggg tagccgaaga
     1441 tcgccagccc gccgttcgcc tgccagaagc gcagcatgtc gccctggatg ctgtggcccg
     1501 tggccgcgaa gtacaggtgg ccggggttgg aggggaacgc ggcgatggtt gggaaggcac
     1561 ggccctggac ggcctcggcg cccaaccggc ccagcgtgat ctggcggccc ggcgggtagt
     1621 cggggtgcca ttccagccgg gcgcgctcca ggtactgcac ggtcagcccg ccctcctgca
     1681 actcgccggt cagcggcgcg cccaacagca gcgccccgtt gtggtcctgc cagaactggg
     1741 cgaaaccgcc gctcaacagc gggctggccg cagggggcag cggcagcggg ctgcgcggca
     1801 cggcctccga cgcggcggcc gggcgcgccc cggccagcag ggcgggcacg atcacgaaca
     1861 ggacgatcag gcgacgtagc acaggcggtt ctcctttagc gctggcgctg actccgacag
     1921 catgcacaat accacgcatt gggctttggc gtccacaagg cgaggctggt gccgcgtccg
     1981 caggctggtg ggcagggcgg atcgcggcgc cggcgcgcgc tgaatttgct tgacaaatgg
     2041 gcgggcaatg ggttagactc gtgccaatta gaaccggctg ctaatcatgc gtccaggact
     2101 ttatgactac tgagcctgca accgaagacc ccaccgcttc acctgccccg cgcctcgcgg
     2161 cggtggtgct cgccgcgggg atgggcacgc ggatgcgctc ccggttgcct aaagtattgc
     2221 accgaatcgc gggcgtgccg ctggtggaac atgtcgcgcg cgccgtggcg ccgctggcgc
     2281 ccgcgcggac cgtgctggtg gtgggccacg gcggcgagca ggtgcaggcc gccctgggtc
     2341 ccggctacga gtatgtgacc caggcgcaac aactgggcac cggccatgcc gttctccagg
     2401 cccgcgccgc cgtcgagggg caggccggca ccgtgctcgt cctctacggc gactccccgc
     2461 tcttgcgctc caccactctg gccgatctcg taagcctcca ccactcgcgg cccggcgtgc
     2521 gcgccaccat tctaacctgc atcgccgagg accccaccgg ttacgggcgt attatccgcg
     2581 acgccaccgg tcaggtcttc gacatcgtcg aggaacgcgt ggcgaccccc gaacaacggg
     2641 ccattaaaga gaccaacagc ggcatctatt gcttcgattc ggcctggctg tggccccggc
     2701 tggccgagtt gccgatccac cccggcgcgg gcgaatatta cctcaccgat atgatcgccg
     2761 tggcgatccg cgaacagtcg ggcagcgtgc aaaccttcca ggtcagcggc atggaagagg
     2821 tcgccgggat caacaaccgc gtccaactgg ccgaggccga tctggtcatt cgcaaccgca
     2881 tccgcacccg ctggatgctc gaaggcgtga ccatgatcga tccggccacg acctacatcg
     2941 acgtagacgt gaccctgggc caggatacga tcctctatcc cggctgctac ctgcaaggcc
     3001 agacccgcgt gggcgccgat tgccgcctgg ggccgcaggc ccgcctggtg gacgccgtgg
     3061 tgggcgacgg ctgcgtggta ggcacatcgc tgctggaaga gtgcgtggtg gaggccggcg
     3121 tcgatatcgg ctcgttcaac cacctgcgcc ccggcgccta cctgtccagc ggggtgcatc
     3181 tcggcaactt cgccgaggtc aagaattccc gcctgggccg caacgtgtct cagggccact
     3241 tcagctacat cggcgacgcc gaagtggggg acaacacgaa catcggcgcg ggcacgatca
     3301 ccgccaactt caacaacggc gtgaagagcc gcacgcagat cggccccgat gtttttatcg
     3361 gctcggatag cgtgctgatc gccccggtcg aagtgggggc gggcgcgcag accggggcgg
     3421 gcgctgtggt gaacaaagac gtgccgcccg gcgcgctggt ggtcggggtg cccgcgcggg
     3481 tcatccgctg gctcaacggc ggcccgccgg agcggggcgg ccccgccacc ccgccgcggg
     3541 aggggagcga cccacccgca tgagtagagc cttgcaggtg ttcagcggca gtgcgaaccg
     3601 gcctctggcc gaggccattt gtcgctgcct gggggtgcgg ctcggcaagg cgattgtggg
     3661 gcgctatcgc aacggcgaaa cccgcgtgcg cgtggacgag agcgtgcgcg gcgatgatgt
     3721 gtatgtcatc cagcccacct cgaaccccgt caacgaaaac ctcatggaac tgctgatcat
     3781 gctcgacgcc ctgcgccggt cgtcggcggc gcgcgtgacg gcggtcatcc cgtactacgg
     3841 ctacgccaag caagagaaaa agaccgcgcc ccgcgagccg attaccgcca aactggtcgc
     3901 caacctgctg gtggaggccg gcgccg
//
LOCUS       DASUYC010000491         3972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_926924,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000491 DASUYC010000000
VERSION     DASUYC010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926924"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..1556
                     /locus_tag="VF276_08070"
     CDS             195..1556
                     /locus_tag="VF276_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606854.1"
                     /translation="MRILALRRLRQSGPATCAAALVLGGLLLAACGSEPARVGNVPAA
                     QAAAHARLQTTPGPTPVVPHPQGNANSSGSFGPSLDSLNLPTPAPTAVPTLPAGPPVA
                     ATQPPSAGVQTRPLPAGQLSVRWRLAYAGLDGQIWTVTRQGTDPRVLLSQPAADGGTG
                     RVIDLNPSPDATRLLYTIVGGPTGPRYFLLDLAAGQVTSVKYNGPWAQDSRHIVTSVN
                     NQLVVADVKAGTEETAGEGNYAGWTADNQIMAVRGGNIWLIPYPASAGAPRQLTDWPA
                     AGDGAWVFDAPLQYHYTNRILFAGAPRNTLNAAGNGLRLHALDVATRRITDLTAPGGN
                     QVRGLALSPSGMQIAIAEQTARTACASPGAISVARPTGNAPAITITLPQAENDFYFLR
                     GLTWSPDQWLAYSAVEQTCARGTPQQVHTDKIYLVNPTNPGKSIPLVDGAFPAWIMPR
                     GLG"
     gene            1590..1955
                     /locus_tag="VF276_08075"
     CDS             1590..1955
                     /locus_tag="VF276_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606855.1"
                     /translation="MLQQIHHTIANMAIYYTFIVGVWGLVLFLRRRGPDGNFNGAVAI
                     AAGIYAVQAVVGLILVFTGLMPANGIHFLYGVSTFLTIPLAFTLTRGRTDSRSSLIYG
                     LAVMFLWGLSLRAFDTAFH"
     gene            2110..3024
                     /locus_tag="VF276_08080"
     CDS             2110..3024
                     /locus_tag="VF276_08080"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX6606856.1"
                     /translation="MLLSLPAARDMRAAALLCLILLPALLVACGGNTADSTPAAPSPI
                     RAVTVAPTPPPAQPTDTALPPTAVPPSPTAAPPTATPGPPPAIAVLAYHHFDVPAAGD
                     YNVPISKFELDLRWLKHNDYESISPQQLVDAIHGRAPLPAHPVMITFDDDNSEQYTEA
                     APLLEKYGYRGAFFIDTVTIGKRYFMTADQLRDLERRGHTIGTHTWDHRDLAILTIDE
                     VKEQLDRSESDMEAVLGHKPLFMSYPFGAYNPEIVAELQARGYHGAFRLHAPDDPVVD
                     PAFMIHRQIIAGVWSVKDFANNVHWMEP"
     gene            3264..3488
                     /locus_tag="VF276_08085"
     CDS             3264..3488
                     /locus_tag="VF276_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606857.1"
                     /translation="MYMTDEYQAIAESTTDWESGTAQMPAARMDYTRVSQASHVEDGY
                     ADEANSDLGNLGRDVHDMAAAAQFEWRQAS"
     gene            complement(3562..>3972)
                     /locus_tag="VF276_08090"
     CDS             complement(3562..>3972)
                     /locus_tag="VF276_08090"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEX6606858.1"
                     /translation="LFIERLAANGIISGYACGGPGEPCDPALRPYFRWGAPATRGQIA
                     KIVVGAAGWSGAAGAQVFEDVPPGHPFYDWIGQAAAHGILSGYTCGSDAEPCIEPARR
                     PYFRPAVAATRGQMSKIIYNAFPGAAPCPAGVEP"
BASE COUNT          657 a         1451 c         1210 g          654 t
ORIGIN      
        1 gtagtactta accgagagtg tgcccaccct gtgacgcgcc tctacaggcg attccgtgtg
       61 ttgacaagcc ctttcaggct atgctatact cttgctggat taacataaat ctaaccgcgg
      121 ttcgttggtg acgcagcggc cctgatccac atcctaatcc ccgtcgagag atcggtccag
      181 gcagaggtga acgtatgcgc atcctagctc tccgccgtct gcggcagagc ggcccggcga
      241 cctgtgctgc tgccctggta ctcggtgggc tgttgctggc ggcctgcggt agcgaaccgg
      301 cgcgggtggg caacgtgccc gccgctcagg cggccgcgca cgctcgcctg caaaccacgc
      361 ccggccccac gcccgtcgtg ccccacccgc agggcaacgc caactccagt ggcagcttcg
      421 gcccctcgct ggactcgttg aacctgccga cgcccgcgcc caccgccgtg cctaccctgc
      481 ccgccggtcc gcccgtagcg gccacgcagc cgccgagcgc cggggtccag acccgacctc
      541 tgcccgccgg gcagcttagc gtccgctggc gcctggccta cgccggcctc gacggccaga
      601 tctggaccgt gacccgccag ggcaccgacc cgcgcgtgtt gctgtcgcag cccgccgccg
      661 atggcggcac gggccgggtt atcgacctga acccctcgcc cgacgccacc cggctcttgt
      721 acactatcgt gggcggccca accggcccgc gctacttcct gctggacctc gccgccgggc
      781 aggtaacctc agtcaagtac aacgggccct gggcgcagga cagccgccat atcgtcacat
      841 cggtcaacaa ccagcttgtg gtcgccgatg ttaaggccgg gacagaggaa accgccggcg
      901 aaggcaacta cgcgggctgg accgcggaca accagattat ggccgtgcgc ggcgggaata
      961 tctggctgat cccctacccg gcgagcgcgg gcgccccccg ccaactcacc gactggcccg
     1021 ccgccggcga cggcgcctgg gtcttcgacg cgccgctgca ataccattac accaaccgca
     1081 tcctgtttgc cggcgcgccg cgcaacacgc tgaacgccgc cgggaatggg ctgcggctgc
     1141 atgcgctcga cgtggccacg cgccggatca ccgacctgac ggcgcccggc ggcaaccagg
     1201 tgcgcggcct ggccctctcg cccagcggca tgcaaatcgc gatcgccgag cagacggcgc
     1261 gcacggcctg tgccagcccc ggtgccatct ccgtggcccg gcccaccggc aacgccccgg
     1321 ccatcaccat caccctgccg caggccgaga acgacttcta cttcctgcgc ggcctgacct
     1381 ggtcgcctga tcagtggctg gcctatagcg cggtcgagca gacctgtgcc cgcggcacgc
     1441 ctcagcaggt ccacaccgac aaaatctacc tggtcaaccc caccaacccc ggcaagtcga
     1501 tcccgctggt agacggcgcg tttccggcct ggatcatgcc ccgcgggttg ggctaatcgc
     1561 gggcggccac ccgcacgtag gaccgattga tgctgcaaca gattcatcat accatcgcca
     1621 acatggcgat ctactacacc ttcatcgtgg gggtctgggg cctggtgctg ttcctgcgcc
     1681 ggcgcggccc cgacggtaat ttcaacggcg cggtggccat cgccgccggg atctacgcgg
     1741 tccaggccgt cgtcgggctg atcctggtgt ttaccggcct gatgcccgcc aacggcatcc
     1801 acttcctgta cggcgtcagc accttcctga ccatcccgct ggcgttcacc ctcacgcgcg
     1861 ggcggaccga cagccggtcc tcgctgattt acgggctggc cgtcatgttt ctctggggac
     1921 tctccctgcg cgccttcgat acggcgtttc attaggcata tccgcgcgcc gcgttgccgg
     1981 tacgccttga cggcggggcg attcttgggt acattgcatt gtccggttgt cccatgcccg
     2041 gcatgaccag ggcggccacc caacaccaca ggtaatcgat cctataccga taccaaccga
     2101 ggtcctgttt tgctcctgtc gctgcccgcg gcgcgcgata tgcgtgctgc cgccctgctc
     2161 tgcctgatct tgctgccggc gctgctcgtg gcgtgcggcg gcaataccgc ggatagtact
     2221 cccgccgcac cctcgcctat ccgcgccgtc acggtcgccc caacgccacc gcccgcccag
     2281 ccgacggata ccgcgcttcc gccgacagcg gtgccgccca gtccaaccgc cgcgccgccc
     2341 acggcgaccc ccggcccgcc cccggccatc gcggtgctgg cctatcacca cttcgatgtg
     2401 ccggcagccg gcgattacaa cgtgccgatc agcaagtttg aattggactt gagatggctc
     2461 aagcataacg attacgagtc gatctcgccg cagcaactgg tcgacgccat ccatggccgc
     2521 gcccccttgc ccgcgcaccc ggttatgatc accttcgacg acgacaacag cgaacagtac
     2581 accgaggcgg cgccgctgct cgaaaaatac ggctatcggg gcgccttctt catcgacacc
     2641 gtgaccatcg gcaaacgcta cttcatgacc gcggatcaac tccgggacct ggagcgccgc
     2701 gggcacacca tcggcaccca tacctgggac caccgcgacc tggcgatcct gaccatagac
     2761 gaggtcaaag agcaactgga tcgctccgaa agcgatatgg aggccgtgct gggccacaag
     2821 ccgctgttca tgtcctaccc cttcggcgcg tataacccgg agatcgtcgc cgagctccag
     2881 gcgcgcggct atcacggcgc tttccgcttg cacgctccgg atgatccggt ggtagacccg
     2941 gccttcatga tccaccgcca gatcattgcc ggcgtctggt cggtaaagga cttcgcaaac
     3001 aacgtccact ggatggaacc ctgatcgccc cgccaaaagc cgcccgcgcc ggctgtggcg
     3061 cgggcgccgg catttttgtg catagcgcac gagattcccg cgccagattc gtcgtgccgg
     3121 atggttgcgg tgtggaatgg atggtgctat aatatcgcca agcacaaagg cgtccttctg
     3181 ccagcgtggg cagtatgggt ccgtggatct gcgcgacccc gctacatgtt cgcgacctgc
     3241 gttcggaaag gagccgcaat tccatgtata tgacggatga gtatcaggcc atcgccgagt
     3301 ccaccaccga ttgggagtcg ggtactgcac agatgcccgc ggcccgcatg gattacaccc
     3361 gtgtcagcca ggcaagccac gtcgaagacg gctacgccga tgaagcgaac agcgacctcg
     3421 gcaatctggg gcgcgatgtc cacgatatgg ccgctgccgc ccagttcgag tggcgccagg
     3481 ccagctaaag cccgctgaaa tgcaaccgcc gcgtgggata cacgcggcgg tttttgttgc
     3541 tcccaacctc ccggcaacgg gtcacggttc cacgcctgcc gggcagggcg cggcgccggg
     3601 gaaggcgttg tagatgatct tgctcatctg cccccgcgtc gcggccacag caggccggaa
     3661 gtagggccgc cgggccggct cgatgcacgg ctcggcatcg ctgccgcagg tgtagccgct
     3721 caggataccg tgcgccgccg cctgcccgat ccagtcgtag aacgggtggc ccggcggtac
     3781 atcctcgaac acctgcgcgc ccgccgcccc actccacccg gcggctccga ccacgatctt
     3841 ggcgatctgg ccgcgggtcg cgggcgcgcc ccagcggaag tagggccgca gggcggggtc
     3901 gcacggctcg cccggcccgc cacaagcgta gccgctgatg ataccgttgg cggccaggcg
     3961 ctcgatgaac ag
//
LOCUS       DASUYC010000492         2817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1701745, whole genome shotgun sequence.
ACCESSION   DASUYC010000492 DASUYC010000000
VERSION     DASUYC010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1701745"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1458
                     /locus_tag="VF276_08095"
     CDS             <1..1458
                     /locus_tag="VF276_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606859.1"
                     /translation="PAFNHWTPAFPAFFLALALPPAHWLRALRQIGLPAWRVGAVAVA
                     GGMVALAVANGYMYLVTYPPTVPVPDSLIAAQGRFLAGLGPQAQARFVGDSWQPYERD
                     MAATLAPTVPAGDLLNPSRALPLPGDPAHDLVFVFYPDQAAYLPVVREYYPGGQLAPV
                     TIPGGTAVAESYRVPAATALARHGVLLTLTGKQAAPPMWQGQVPAVGALPASVAVHYP
                     VTTTWTGLLYVADPAPLRLEVTGSAGARLWLQDAPVGVGETLTVDPGWVPFRLEAALA
                     GPAALHLRMQQSRGAAQDVPATYLWPQAPDAGLAVTFAGSAAPGRIDPFIGASMFGTA
                     DSPALVGVIPELLRRDPDFVPLGPRTGGGTPLRWDGEVYAEGGTYTMALRTDARARLR
                     IDGAPVLDLCANVAPPPQIPRFAPYPWHEAPITLSRGWHTVAIDFDATGYNNGLEWAW
                     TRPDGVREIVPPSRLRHSAPAPPGHGDAPAGCPAR"
     gene            complement(1571..2512)
                     /locus_tag="VF276_08100"
     CDS             complement(1571..2512)
                     /locus_tag="VF276_08100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012850888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03564 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX6606860.1"
                     /translation="MRIGILIGTADGQSVPMADLVMQARQAEADGFASGWFANIFGMD
                     ALTAAAVCGQATSRLELGTAVVPTYPRHPYALAQQALSTQAAAGGRLLLGIGPSHQPV
                     VEWIWGLSFKKPVAVLREYLEVLLPLLERGQVAFQGETYRVAAELKVPGATPCPVLLG
                     GLGPRMLALAGTMCAGTVTAWVGPRTLAEHVVPSIREAAAAAGRPAPRIAAMVSVGVT
                     ADVAGTRERVARGSRMYGELPSYRAMLDREGVAGPADVVVLGDEDTVAAQIRHFGDIG
                     VTDLIAVPVPGLPDRGETLARTRALLVQLAHETPPAV"
BASE COUNT          372 a         1067 c          974 g          404 t
ORIGIN      
        1 cccgccttca accactggac gcccgcgttc ccggcgttct tcctggccct ggccttgccc
       61 ccggcccatt ggctgcgcgc gctgcgccaa atcggactcc cggcatggcg cgtgggcgcc
      121 gtggcggtgg cgggcggcat ggtcgcgctg gccgtcgcca acggctatat gtacctggtc
      181 acgtacccgc ccaccgtgcc ggtgccggac tcgctgatcg cggcgcaggg ccgcttcctg
      241 gccgggctgg ggccgcaagc gcaggcgcgg ttcgtgggcg actcctggca gccctacgag
      301 cgcgacatgg cggccacgct ggcgcccacc gtgcccgcgg gcgacctgct caaccccagc
      361 cgggcgctgc cgctgccggg cgatcccgcg cacgacctgg ttttcgtctt ctaccccgac
      421 caggcggcct atctgcccgt cgtgcgggag tattatccgg gcggccaact ggcgccggtg
      481 acgatccccg gcggcactgc ggtggccgag tcgtaccggg tgcccgcggc cacggccctg
      541 gcccgccacg gcgtgctcct tactctgacc ggcaagcagg ccgcgccacc catgtggcag
      601 ggtcaggtgc cggccgtggg ggcgctcccg gcgagcgtcg ccgtccatta ccccgttacg
      661 acgacgtgga ccggcctgct atacgtggcc gatcccgcgc cgctgcgcct ggaggtgacg
      721 ggaagcgcgg gcgcccggct ttggttacag gacgcgccgg taggcgttgg ggagacgcta
      781 accgtcgatc ccggctgggt gcccttccgc ctggaggcgg cgctggccgg cccggcggcg
      841 ttgcatctgc ggatgcaaca gagccggggt gcggcgcagg acgtgccggc cacgtacctc
      901 tggccgcaag cgccggatgc cgggctggcc gttaccttcg ccggatccgc cgcgccgggc
      961 cggatcgacc cctttatcgg cgcaagcatg ttcggcaccg ccgacagccc ggccctggtg
     1021 ggcgtgatcc ccgaactgct gcggcgcgac cccgacttcg tgccgctcgg cccgcgcacg
     1081 ggtggcggca ccccgctgcg ctgggacggc gaggtctacg ccgagggcgg gacctacacg
     1141 atggcgctgc gcaccgacgc ccgcgcccgc ctgcgcattg acggcgcccc ggtgctcgac
     1201 ctctgcgcga acgtggcccc cccgccgcag atcccccgct tcgcgcccta tccctggcac
     1261 gaggcgccta tcaccctcag ccggggctgg cacaccgtgg cgatcgactt cgacgccacc
     1321 ggctacaaca acggcctcga atgggcctgg acgcggcccg atggcgtgcg cgagatcgtg
     1381 ccgccgtccc gcctgcgcca cagcgccccc gccccgccgg ggcacggcga tgcgccggcc
     1441 ggttgcccgg cccggtgagc gcgcgttggt gccgcttcca agaacctttg ggaaccgtgc
     1501 ccgctaacgg ccaacgagta cgggcgcgat caatcgcgcc gctacacacg agcggtcaat
     1561 gggatctgcg ctagaccgcg ggcggcgtct cgtgcgccag ttgcaccagc aaggcgcggg
     1621 tgcgggccag cgtttcgccc cggtcgggca ggcccggcac cggcacggca atcaggtcgg
     1681 tcacgccgat gtcgccgaag tgccggatct gcgcagccac cgtgtcttcg tcgccgagca
     1741 ccaccacgtc ggcgggcccg gccacaccct cgcggtcgag catggcgcgg taggagggca
     1801 gctcgccgta catgcggcta ccgcgcgcca cccgttcgcg ggtgcccgcc acgtcggcgg
     1861 tgacgcccac gctgaccatg gcggcgatgc gcggcgcggg ccgcccggcg gcagccgcgg
     1921 cctcgcggat gctgggcacg acgtgctcgg ccagggtgcg cggccccacc caggcggtca
     1981 cggtcccggc gcacatggtt cccgccagcg ccagcatgcg cggtcccagc ccgcccagca
     2041 acaccgggca gggcgtggcg cccggcacct tcagctctgc ggctacccgg tacgtctcgc
     2101 cctggaaggc cacctggccc cgctccagca gcggcagcag cacctccagg tactcgcgca
     2161 gcaccgcgac gggtttcttg aacgacaggc cccagatcca ctcgacgacc ggctggtgtg
     2221 acggcccgat gcccagcagc agccgcccgc ccgccgcggc ctgggtcgac agcgcctgct
     2281 gggccagggc gtaggggtgg cgcgggtacg tgggcacgac cgccgtgccg agttcgaggc
     2341 ggctcgtggc ctgcccgcac accgccgccg ccgtcagcgc gtccatgccg aagatgttgg
     2401 cgaaccagcc cgacgcgaag ccgtcggctt cggcttgccg cgcctgcatc accaggtccg
     2461 ccatgggcac cgattgcccg tccgccgtgc cgatcaagat gccgatgcgc ataattgctc
     2521 ctcctctgcc gcgtggcgcc tactatacac ccgtgcccgc ccggcgtcaa tcggcgcccc
     2581 ccgattgcac cgccgaggcg cagaggacgc agagaacgat ccagggtttg attacgacgg
     2641 aggccaccgc gagggcgcga aggccgcaga ggatttaggg gtttcactcc tagcgaataa
     2701 ttcttacaat cctgcgcgtt cttcgcgcct tcgcggtgaa tatccataaa aacctcggcg
     2761 ttctctgcgt cttccctgaa agggggaagg acgggtagcc ctggggactt gtcattc
//
LOCUS       DASUYC010000493         3183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1386119, whole genome shotgun sequence.
ACCESSION   DASUYC010000493 DASUYC010000000
VERSION     DASUYC010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1386119"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /gene="tal"
                     /locus_tag="VF276_08105"
     CDS             complement(<1..842)
                     /gene="tal"
                     /locus_tag="VF276_08105"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEX6606861.1"
                     /translation="MVTQLQRLVAAGQSPWYDDIHRGLMKSGRLQDLVTQGIRGATVN
                     PTIFAKAVAGSTDYDSEIQELLGRGLGPAEIYERLLIEDVRSAADLFRPLYDETGGAD
                     GYASIEVSPYLAANTQGTIEEARRFFRSVDRPNVMIKVPATNAGVPAIRRLTGEGINV
                     NITLIFGIEYYEKVMDAYIGGLQDLQTRGRSLDKVASVASFFVSRVDSEVDKRLDAIA
                     ASGDAATKDRAMALKGRAAIANAQLAYQLFTEMFSGDRWAKLAAKGARLQRPLWASTS
                     TKNPA"
     gene            complement(1153..1563)
                     /locus_tag="VF276_08110"
     CDS             complement(1153..1563)
                     /locus_tag="VF276_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEX6606862.1"
                     /translation="MTYVPPQQWPEEGVYLDSAVELNLVQVRARLTALRKQLEFDDPF
                     PDGPADPASGEHAVVFAVQAANAHKLAQVRAALARINDGTYGTCADCTQPIGGARLAA
                     RPFAIRCVACQQLADRQANQGAGWSLPPSQWLAN"
     gene            complement(2405..2626)
                     /locus_tag="VF276_08115"
     CDS             complement(2405..2626)
                     /locus_tag="VF276_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6606863.1"
                     /translation="MKDEILVPLDGSPLAEAILPHAVMLARTTARSITLLRAVASPMN
                     GGAAALWTPTYTCPTCNHGRLRLTRPPGI"
     gene            complement(2771..>3183)
                     /locus_tag="VF276_08120"
     CDS             complement(2771..>3183)
                     /locus_tag="VF276_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014369596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEX6606864.1"
                     /translation="RGSWAATENIIPATSGADKALKFIWPDLNVTGKAYRVPVRTGSI
                     VELNAILGREASAAEVNDAFRRAAAQAPLQGIMGVLEDEWASSRILGEPHSSLVDLPL
                     TQVLGGRLLTVAAWYDNEMGYAIRLAEMAQAMAG"
BASE COUNT          526 a         1039 c         1026 g          592 t
ORIGIN      
        1 gccgggttct tcgtgctcgt actggcccag agtggacgct gaagacgcgc gcctttcgcg
       61 gccagctttg cccaacgatc gcccgagaac atctccgtga acagctggta tgcgagctgc
      121 gcgttggcga tcgccgctct gcccttgagc gccatcgcac ggtctttcgt agcagcgtca
      181 ccgctcgcag caatcgcgtc gagtcgcttg tcgacctcgg aatccacgcg gctcacgaag
      241 aagctggcga cggatgctac cttatcgaga gatcggcccc tcgtctggag atcctgcaga
      301 ccgccgatgt aggcatccat taccttttcg tagtactcga taccgaaaat tagcgtaatg
      361 ttaacgttga tgccctcgcc cgtaagccgg cgtatcgcgg gcactccggc attggtggca
      421 ggtactttga tcatcacgtt gggccggtct acgctacgga agaacctgcg tgcctcctcg
      481 atagtgccct gcgtgttcgc ggccaggtag ggggatacct cgatgctggc gtacccatcg
      541 gcgccgccgg tctcgtcata tagtgggcgg aacaggtcgg ctgcagaccg gacgtcctcg
      601 atcaacagcc gctcatatat ttccgccggg cccagtcccc gacctagtaa ctcttgtatt
      661 tcgctatcgt agtccgtgct gccggccact gccttcgcaa agattgtggg gttcacggtc
      721 gccccacgta taccctgcgt gacaaggtcc tgtagccggc ctgatttcat gaggccgcga
      781 tgtatatcat cgtaccaagg gctttgcccc gccgctacca gtcgttgcag ttgagtaacc
      841 atagcctcgc tatctcctct cagttttgcg ctatctgcac ctcgtagtct cacctttctc
      901 agcgatctgc cagggcagcc gcgaccgcgg cgccgataaa cccatcgcct atgacggcga
      961 accgctccgc acgctcggta agcgcgcgca ggcgcagacc agaacttctt agaaagcggc
     1021 gcacgcaggt aaggcatgtc gctctcttcg cggatcagcg ttatagcacc cgtcgggtcg
     1081 acctcgcgaa tgccgccccc ggcggcggaa gcgatcatgc cgccttccga tttacccccg
     1141 ccgggggcga aattaattcg ccagccattg cgaagggggg aggctccaac cagccccctg
     1201 gttagcttgc cggtccgcca attgctgaca agcaacacag cggatggcga aggggcgggc
     1261 cgcgagccgg gcgccaccga tcggctgggt gcagtccgcg caggtgccat aggtgccgtc
     1321 gttgatgcgc gccagggccg cacgcacctg cgccagtttg tgcgcattgg cagcctggac
     1381 ggcgaagacc accgcgtgct ccccgcttgc cggatcggcg ggcccatccg ggaatgggtc
     1441 gtcaaactcg agttgctttc ggagtgccgt caggcgcgcc cgcacctgga ccaggttgag
     1501 ttcaaccgcc gagtccaggt agactccttc ctcgggccat tgttgcggcg ggacgtaggt
     1561 catgtgagaa cctccaatag caccgcgcca taggcgcggc caaaagctgg gattgggccg
     1621 ggtggactgg tgcgcggccc ggactctctc gcaggtcgtc aatcgccggg tacattcctg
     1681 cgggatggca agctgtgacc ccagcaaagg acccgccggc agccgggctt cctctcgcca
     1741 gatggaggaa acctgcgcgc ggcaccggcc cggattacgg cgaccccgcc gtagtccggc
     1801 tccaggtgca acgcggccca ccacaagagc gctgccggcg tccgttgccg tcggaatggg
     1861 tccttctatg ctggattata gtgggggact ttaaagatag cgggcgggag agtgttaagg
     1921 ttgcgttaag attcgcccgg ccgcttcgtt ttcaagcggg gtgagcggcg gagcggcgcc
     1981 gctatgccgt gccccggctt tggggccggt cccgctaatc gcgaaagcac ggccggggct
     2041 gtaagaggcc ggcgcctgtg gcggcggcga ggttccgggt ataagccagc acctgcgccg
     2101 ccagcgaaga tccatcgagc ggcaccagga tctttgtgta cggcacgacc ggctcccacg
     2161 cggcgccggc gtcggggaag gctgggcgca ccactagcag gggggtccgt cccctgcacg
     2221 accttttccg ccacactccc caatcaccag cgggtaaagc cggtacgacc atgcgtcgcg
     2281 agggcgatta gccgcacgtc cgggctctgg gtggcatagt ccaaaatgga tgcggccggg
     2341 gcgttcgttg ccattcgtat ggtcactgcg gcaccccgat cgcgtaggcg ccgcgcgacc
     2401 tggttcagat acctggtggc cgagtcaacc tcagcctccc atggttgcag gtcgggcacg
     2461 tgtatgtcgg cgtccagagc gccgctgcgc cgccgttcat tggtgaagcc accgcgcgca
     2521 gcagcgtgat gctgcgcgcg gtggtgcggg ccaacatcac cgcgtggggt aggattgcct
     2581 cagcgagcgg ggagccgtcc agtggcacga gaatctcgtc tttcatggcg tttgtctcct
     2641 tgtgcgacgg tagtcgaaag ctggagccgg cagcgccacc cactcccgct ggtggcgctg
     2701 ccgcctacgc gatcggcttt cgtcctgctg tatcccggat tcgcaacggg cttgaccagc
     2761 ggagaccggc tcaccccgcc atggcctgcg ccatctccgc gagccggatt gcgtaaccca
     2821 tttcgttgtc gtaccaggcg gcaacggtga gcagccgccc gcccagcacc tgggtcagcg
     2881 gcaggtcgac cagcgaggag tgcggctcac ccaggatgcg gctggaggcc cattcgtctt
     2941 ccagcacgcc catgatgccc tgcaaaggtg cctgcgcggc ggcgcggcgg aaggcgtcgt
     3001 tgacctccgc cgcactggct tcccggccca ggatcgcgtt caactcgaca atgctgccgg
     3061 tgcgcacggg cacgcggtac gccttccccg tgacgttcag gtccggccag atgaacttga
     3121 gagccttgtc cgcgcccgac gtggcgggga tgatgttctc ggtggcggcc caggagccgc
     3181 ggc
//
LOCUS       DASUYC010000494         4248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_800380,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000494 DASUYC010000000
VERSION     DASUYC010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800380"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            355..1233
                     /locus_tag="VF276_08125"
     CDS             355..1233
                     /locus_tag="VF276_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020580402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX6606865.1"
                     /translation="MIVVPEEFAERTIAREGAAGRAWIAALPERVAALVGQWGLAVDG
                     PPMHGGLSLVVPVRRDAEPCVLKIAWVDVETEAIALRAWDGRGAVRLLDAALEQHALL
                     LERLDYGRTLNDVDLAEAIDIAGRLLRRLAIPAPPGLHPLPARAGDIARDLPGLWERT
                     GHPLPRPVLDKVHDLAARLAVTRPAHLLVNFDLYYDDVLAGDREPWLVVDPMVVAGDP
                     EYGIGPLLWRRLEDIEAQGGIVRHFNAIIDAAELDPDLACEWTLVRCVEYWLWGLGAG
                     LTYDPARCARIVEALL"
     gene            1373..2722
                     /locus_tag="VF276_08130"
     CDS             1373..2722
                     /locus_tag="VF276_08130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEX6606866.1"
                     /translation="MQTVIRNPKARLRGRHLAGLLVILAAAMLLCWMNRPWFAGWVDQ
                     ATLPAARATYNLNDYAAFANAARMILAGHGPDLYDIEAQRATELRDWGARFDPQLAVP
                     FVNPPWFAVALLPLAPLPMGAGFLIWLAICCALILGAAALLARWARPPPLVGVAFVLS
                     VAGFVPFWRTAILGQTSALVLFGLAWGVVSVAEGRARRGGLALALAGVKPQLLILPVL
                     ALVMERRRKALFWCVSASLALVAVTLPLIGLGAIPDYLTLMRSAAYAGYENLPQMQSW
                     RALVEGTLGLRGSPATVLNLAGMAAGLGLVVWAWWEPARTRWAGGPRHWTRLIERNPV
                     QSDPNWDLRMALTILATQLFTPHMHVHDLLIWIVPGGLLLHHLYAPGAAWRMGRRRRT
                     VGFVTLWAGYLVVWPAWLLPDARLALWFSLLVCGWLVAELRAARQERAPTWARTATT"
     gene            2698..4020
                     /locus_tag="VF276_08135"
     CDS             2698..4020
                     /locus_tag="VF276_08135"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEX6606867.1"
                     /translation="MGQNRDDLIQVSGMGPGFFTKLGAACCLLLLVGYGILWSGTVQR
                     SGGADGYVRHTEFLTTMTGAAVVAGPQPALLYDHDTQAAAQTDLLSGEADPGRLLPYT
                     APPFAAVLLAGPVRAGASASVLFTLWAVLTTTAAGLCIGLLAGRWAAPQGTPWMLMLA
                     CTSFLPLISALMSGQNLMPAILGWVGVTVGLKSGRDGVAGAAGALAVLAPAILPLLLL
                     LLLITRRWRALAALAATLALAALAVAPLLGVDWPLRYGHWLLESAGLPGASGLPSILP
                     WPAPAAGAAGVDSGTVLAAGCALLVLLWTWLPWQGGAVWRPATVGWDLRWAISLIAML
                     LPALSLQPNALALAIIPGWILGTHLATGRLRSPRRVYWLAWLWIGYALGMAATLAAGG
                     ARALVIVWLVGALAGLAWNLWSRRVDEPERPEPPGGRPDAPWPELSGG"
     gene            complement(4027..>4248)
                     /locus_tag="VF276_08140"
     CDS             complement(4027..>4248)
                     /locus_tag="VF276_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606868.1"
                     /translation="AAALAGIFLSLVFLLMHGGDRLIHRLRRRPAVCPRCAATEAGSS
                     LRFRRTPVEGTGWEEITCPQCGHAWYGRT"
BASE COUNT          515 a         1456 c         1579 g          698 t
ORIGIN      
        1 atccccgccg gtgcgcctgc cccccgtgtc actctgagcg cagcgcagag tctcgtcttg
       61 gacccgcacg cgacgagatt cttcgctgcg ctcagaatga caaaatgaca agagaaacgc
      121 catgcccctc gttggttgtc actctgagtg cagcgaagag tctcgtctcc tcgaccttgt
      181 cactctgagc gcagcgaaga gtctcgccgc cggggtgtat tctgccccaa cgagagcagt
      241 gatttgcacc cgtagcgttt ggtgaaacgt aacaaatacg agggaaatgg gtataccaag
      301 tagggagtcg tggcgaaaag gcgcgccgcg ggcggcggca aggccaggtg gtatatgatc
      361 gtggttccgg aagagttcgc tgagcggacg atcgcgcgag aaggggccgc cgggcgggcg
      421 tggatcgccg ccctgccgga gcgcgtcgcg gccctcgtcg gccagtgggg gctggcggtg
      481 gacgggccgc cgatgcacgg cgggctgagc ctggtagtgc cggtgcgccg ggacgccgaa
      541 ccctgcgtgc tcaagatcgc ctgggtcgac gtggagaccg aggccatcgc cctgcgcgcc
      601 tgggatgggc ggggcgccgt gcggctgctg gacgcggccc tggagcagca cgcgctgttg
      661 ctggagcgcc tcgattatgg ccggaccttg aatgatgtcg atctggccga ggccatcgac
      721 atcgcggggc ggttgctgcg gcggctggcg atccccgccc cgcccggcct gcacccgctg
      781 ccggccaggg caggcgacat cgcgcgcgat ctgcccggcc tctgggagcg taccgggcac
      841 ccgctgccgc gcccggtgct ggataaggtg cacgatctcg cggcccggct cgccgtcacg
      901 cgcccggcgc acctgctggt caatttcgac ctgtactacg acgacgtgct ggcgggcgac
      961 cgcgagccct ggctggtggt cgatccgatg gtcgtggccg gcgatccgga gtacgggatc
     1021 gggccgttgc tgtggcggcg cctggaggac atcgaagccc agggcggcat cgtgcgccac
     1081 ttcaacgcga taatcgacgc cgccgaactc gatcccgatc tggcctgcga atggacgctg
     1141 gtgcgctgcg tcgagtactg gctgtggggg ttgggcgcgg ggttgaccta cgacccggcc
     1201 cgctgcgcgc gcatcgtcga ggccttgctg tgagcggcaa tcggcagggt gatggcacgg
     1261 tgttcgcttt gtagggcaac gccgcttgcg tgtataatgc cctttcgggt gccgagtgcc
     1321 gccggccgga aggatatagg accgtatccc gagttggaaa gtgcaatacg tagtgcaaac
     1381 cgttatccgt aatcccaaag ccagactgcg cgggcgtcac ctggccgggc tgctcgtcat
     1441 cctggcggcg gccatgctgc tctgctggat gaaccggccc tggttcgccg gctgggtcga
     1501 tcaggccacc ctgcccgcgg cgcgagccac ctataacctc aacgactatg cggccttcgc
     1561 caacgccgcg cgcatgatcc tggccgggca cgggcccgac ctctacgaca tcgaagccca
     1621 gcgcgcgacc gagctacgcg actggggcgc gcgcttcgac ccgcaactgg ccgtgccgtt
     1681 cgtcaatccg ccgtggttcg ccgtggcgct gctgccgctg gcccccttgc ctatgggcgc
     1741 gggcttcctg atctggctgg cgatctgctg cgcgctgatc ctgggcgcgg cggcgttgct
     1801 ggcacgctgg gcgcggcccc cgccgctggt cggcgtcgcg ttcgtcctgt ccgtcgccgg
     1861 gttcgtgccc ttctggcgca cggccatcct ggggcagacg agcgcgctgg tgctgttcgg
     1921 gctggcctgg ggcgtcgtca gcgtggccga gggccgcgcc cggcgcggcg ggctggcgct
     1981 ggccctggcc ggcgtcaagc cgcagttgtt gatcttgccg gtgctggccc tggtcatgga
     2041 gcggcggcgc aaagccctgt tctggtgcgt cagcgcgagc ctggccctgg tcgccgtgac
     2101 gctgccgctt atcggcctcg gcgccatccc cgattatctg accctgatgc gcagcgcggc
     2161 ctatgccggg tacgagaacc tgccgcagat gcaaagctgg cgcgccctgg tcgagggcac
     2221 gctgggcttg cgaggcagcc ccgccacggt gctcaacctg gcgggcatgg ccgccggtct
     2281 gggcctggtg gtctgggcct ggtgggaacc ggcgcgcacg cgctgggcgg gcggcccgcg
     2341 ccactggacc cggctgatcg agcgaaatcc cgtgcagagc gacccgaact gggacctgcg
     2401 catggcgctg accatcctgg cgacccagtt gttcacgccg cacatgcatg tccacgacct
     2461 gttgatctgg atcgtgccgg gcgggctgct gttgcatcac ctctacgcgc cgggcgcggc
     2521 gtggcgcatg ggccggcggc ggcgcaccgt ggggttcgtc acgctgtggg ccgggtacct
     2581 ggtggtctgg ccggcctggc tgttacccga tgcccggctg gccctctggt tcagtctgct
     2641 ggtttgcggc tggctcgtcg ccgagctgcg cgcggcccgc caggagagag cacccacatg
     2701 ggccagaacc gcgacgacct gatccaggtc agcggcatgg ggccggggtt tttcacaaaa
     2761 ctcggcgcgg cctgctgcct gttgctgctg gtgggctacg gcatcctctg gagcggcacc
     2821 gtgcagcgca gcggcggcgc cgacggctac gtccgccaca ccgagttcct gaccaccatg
     2881 accggcgccg ccgtcgtggc cgggccgcaa ccggcgctgc tctacgacca cgacacccag
     2941 gccgccgccc agaccgacct gctgagcggc gaggccgatc ccggccggct cctgccctac
     3001 accgcgccgc cgtttgccgc cgtgctgctc gccgggcccg tgcgcgcggg cgcctcggcc
     3061 agcgtgctgt ttaccctgtg ggcggtgctg accaccacgg cggcggggct gtgtatcggc
     3121 ttgctggccg ggcgctgggc cgcgccgcag ggcaccccct ggatgctgat gctggcctgc
     3181 acctcgtttt tgccgctgat ctcggccctg atgagcgggc agaacctgat gcccgccatc
     3241 ctgggctggg tgggggtgac ggtgggcctg aagtcgggcc gggacggcgt ggccggggcc
     3301 gccggagcgc tggcggtgct ggcgcccgcg atcctgccgc tgttgctgct gttgctgctg
     3361 atcacccggc gctggcgcgc gctggccgcc ctggcggcga ccctggccct ggcggcgctg
     3421 gcggtggcgc cgctgctggg cgtggattgg ccgctgcgtt acggccattg gttgctggaa
     3481 agcgccggcc tgcctggcgc gtccggcctg ccgagcatcc tgccctggcc cgcgcccgcc
     3541 gcgggcgccg ccggggtgga ctccggcacc gtgctggccg cggggtgtgc cctgctggtg
     3601 ctgctctgga cctggctgcc ctggcagggc ggcgcggtct ggcgcccggc caccgtgggg
     3661 tgggacctgc gctgggccat cagcctgatc gccatgctct tgcccgcgct cagcctgcaa
     3721 cccaacgccc tggccctggc gatcatcccc ggctggatcc tgggcaccca cctggcgacg
     3781 gggcggctgc ggtcgccgcg gcgggtgtac tggctggcct ggctctggat cggctatgcg
     3841 ctcggcatgg cggccacgct ggcggcgggc ggggcccgcg cgctggtgat cgtgtggctg
     3901 gtgggggcgc tggccgggct ggcctggaac ctatggagcc ggcgcgtgga cgagcccgaa
     3961 cggcctgagc cgcccggcgg acgccccgac gcgccctggc ccgagcttag cggcggctaa
     4021 ccgcgctcac gtgcgcccgt accaggcgtg gccgcactgc gggcaggtga tctcttccca
     4081 gcccgtgcct tcgaccgggg tgcgccggaa acggaggctg ctgccggcct cggtcgccgc
     4141 gcagcgcggg cagacggcgg gccgccgccg cagccggtgg atcagtcggt cgccgccgtg
     4201 catcagcaaa aagacaagcg agaggaagat cccggcgagg gcagcggc
//
LOCUS       DASUYC010000495         4287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1409039, whole genome shotgun sequence.
ACCESSION   DASUYC010000495 DASUYC010000000
VERSION     DASUYC010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4287
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1409039"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2623
                     /locus_tag="VF276_08145"
     CDS             <1..2623
                     /locus_tag="VF276_08145"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6606869.1"
                     /translation="EASTGRWEAHPDLMPAAFARQEAILRAAIAAQEGWAYKQIGDAF
                     QAAFQTAPAALVAALAAQRALAAEPWPEAIGPLRVRMALHTGVAEERADDYVGPPLYR
                     AHRLLSAGHGGQILLTAATYQLVRDALPGEVGLLDLGEHRLKDLIRPEHIWQVTPTGH
                     ADAFPPLKTLDARSHNLPVQPTPLIGRETELAAVAQLLRRPAVRLVTLTGAGGTGKTR
                     LALQAAADRLEDLPDGAWFVNLAPLSDPALVPSAIATALDVREAPGRSLLQAVQDYLR
                     DKRLLLVLDNFEQILVAASVVAELLAGVAGLQVLVTSRAPLHLRGEHEFPVPPLALPP
                     GAAAPTVEGLGQYAAVRLFIASALAVKPDFTVTNATAPAVAEICARLDGLPLAIELAA
                     ARSKLLGPEALLARLERRLPLLTGGARDLPTRQQTLRAAIAWSYDLLDAEEQRLFRRL
                     AVFQGGATITAIAAVCNADRDVALDVLDSVGSLVDKSLLRQETAAGSEPRFGMLETIH
                     EYARERLGESGEEPALQKLHARFFLALAEATQPSYYNIRWREKLDRVEADYDNIRAAL
                     DWALEDASHEQTAIRKAELALRLAGALPTFWMSRGHLGEGRRWLERALAHSTALESIA
                     ARAKALTGAGSLAGLCGDNEQAIKHLEEALRLYDQLGDKSGMVDVLLYLGNARVGGGD
                     LASQRALYEQSLALSRELDKKPGIAASLNNLAALAQREGDYQRARPLYEESLALGRAL
                     EFSEISVTLCHLGQIAHREGDYQAAGGYFREALLTARQAQDSLRFGLGLDGLAGIAGA
                     MGQLARAARLYGAVDALHSAIGFTLQGVNRVEHERNLAAARAQLDAANWAASYSAGQA
                     MALDEAVAYALEEPVED"
     gene            complement(2663..>4287)
                     /locus_tag="VF276_08150"
     CDS             complement(2663..>4287)
                     /locus_tag="VF276_08150"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6606870.1"
                     /translation="RFSMLETIQEHAWARLAASGEGDAVQTQHAGYFLALAEEAEPLL
                     KGAPQERWLARLDAEHDNLRSALAWTQNQKQIESGLRLAGALWRFWYIRGYYSEGRAQ
                     LSAVLSQAGAVEPAPEDATLTAARARALHGAGVLAWGQGDYGTATAYSQESLALHRAL
                     NDPAGQANALNTLGLVARSQGDFPQARTLHTESLTLRRSLGDQWGVANALLNLGVVAW
                     NQAAYAEARRLLEESLALDRALGDRWGSAVVLDNLGMVLRSQGDLPRARALLEQSLAF
                     RRALGDRWGIATALQHLAGVAQAQGSTEEAQALLDESLVLYRDVGDINSSALTLRDLA
                     VLAADKDDYRSAQAWLAESLEIWQVAGTPAGIAQGLECAAWLAGRQGRLAEAARLWGA
                     AEALREARGVPMPLVARTAYDQAVAATRAQVDTATWAAAWAAGRALDSAAAVADALDS
                     AGTTAGAVAPVSGAPAPAAERPGGEYPAGLTAREVAVLRLVAQGLTNVQVARELALSP
                     HTVNVHLRSIYSKLDVPSRAAATRFAVDHKLL"
BASE COUNT          681 a         1431 c         1486 g          689 t
ORIGIN      
        1 cgaagcgagc acggggcgct gggaagcgca tcccgatctg atgcccgccg cgttcgcccg
       61 ccaggaagcg atcctgcgcg cggcaattgc cgcccaggag gggtgggcct acaagcagat
      121 cggcgacgcc ttccaggccg ccttccagac ggcgcccgcc gccctggtcg ccgcgctcgc
      181 cgctcagcgt gcgctggccg cggaaccatg gccggaggcg attgggccgc tacgcgtgcg
      241 catggccctg cacacgggcg tcgctgagga gcgcgccgac gactatgtcg gcccgccgct
      301 ctatcgggct caccgcttgc tgagtgccgg ccacggcggc cagatcttgc tcactgccgc
      361 cacctatcaa ctcgtgcgag atgccttgcc tggagaggtc ggtttactgg acctaggtga
      421 gcaccgcctg aaggacttga tccggcccga gcacatctgg caagtgacac ccacggggca
      481 cgccgacgct ttccccccgc tcaagacgct tgatgcccgg tcccacaacc tgccggtcca
      541 gcctactccg ctgatcggcc gggaaaccga gcttgcggcg gtcgcccaac tcctgcgccg
      601 gcccgcggtc cgcctggtga cactgacagg ggcgggtggc acgggcaaga cgcgcctggc
      661 gctgcaggcg gccgccgatc ggctggagga tctgcccgac ggcgcctggt ttgtcaattt
      721 ggctccgctc tccgacccgg cactggtccc ctcggctatc gccacggctc tggacgtgcg
      781 cgaggcgccc ggccgatcgc tgctgcaagc cgtgcaggac tacctgcgcg acaagcgcct
      841 gctgctggtg ctggacaact tcgagcagat cctggtggct gcctccgtcg tggccgagct
      901 gctggccggg gtcgcgggcc tccaggtgct agtgaccagc cgcgcgcccc tgcacctgcg
      961 tggcgagcat gagttccccg tcccgccgct ggcgttgccg cccggcgccg ccgcaccgac
     1021 ggtcgaaggg ctgggtcagt atgcggcggt gcgcctgttt atcgcgtctg cgctggccgt
     1081 taagcctgac ttcacggtga ctaatgctac cgcgccggcg gtggccgaga tttgcgcccg
     1141 gctggatggg ctgccgctgg caatcgagtt ggcggcggcg cgcagcaaac tgctgggacc
     1201 ggaggcgtta ctggcccggc tggagcgccg attgccgctg ctgaccggcg gggcgcgcga
     1261 cctacccacc cgccagcaaa cattgcgggc cgctatcgcc tggagctacg atctgctgga
     1321 cgcagaggaa cagcggttgt tccggcggct ggccgtgttc cagggtggag cgacgatcac
     1381 agcaatagca gcggtgtgca acgcggaccg cgatgtagcg ttggacgtgc tggacagcgt
     1441 gggatcgctg gtggacaaga gcctgctgcg acaagagacg gcggcgggta gcgagccccg
     1501 gttcgggatg ctggagacca tccacgagta tgcccgcgag cggttggggg agagtgggga
     1561 agagccggct ttgcagaagt tgcatgcacg cttcttcctc gctctggccg aggcaacgca
     1621 accgagttat tacaacatcc gatggcggga gaagctggat cgcgtggaag ccgattacga
     1681 caacatacgg gctgcgctag actgggcgtt ggaggatgcg tcgcacgagc aaacggccat
     1741 acggaaggct gaattggctt tacgcctagc cggtgcgttg cctacttttt ggatgagtcg
     1801 tggacacctg ggtgaagggc ggcgctggtt ggagcgggca ctggcgcaca gtacggccct
     1861 ggaatcgatc gcggcacggg ctaaggcgct aaccggggca ggatcgctgg ccgggctctg
     1921 cggagataac gagcaggcca tcaagcactt ggaagaagcc ctgcgcttgt atgatcaact
     1981 gggcgacaag tcggggatgg tggacgtttt gctctatttg ggaaacgccc gagtgggcgg
     2041 aggcgatctc gcatcgcaac gcgcgttata cgaacaaagc ctggcgctgt cgcgagaact
     2101 ggataagaaa cccggtattg ccgctagcct gaacaatctg gcggcgctgg cacaacgaga
     2161 aggagactac cagcgagcac ggcccttgta cgaagagtcg ttagcgctag gtcgtgccct
     2221 cgaattttcg gagatatcgg tgaccctctg ccacctgggg cagatcgcgc atcgagaggg
     2281 cgattatcag gcggcgggag ggtactttcg agaggctttg ctcaccgccc ggcaagcgca
     2341 agacagtctc cgcttcggct tggggctgga cggattggct gggatcgccg gggcgatggg
     2401 gcagctggcg cgagcggccc ggctctatgg ggccgtggat gctctgcact cggctatcgg
     2461 tttcaccttg caaggggtta atcgcgtcga gcacgagcgc aacctggctg cggcgcgcgc
     2521 tcaactcgac gccgctaact gggcggcgtc ctactcggcg ggccaggcga tggcgctgga
     2581 cgaagcggtc gcctacgcgc tggaggaacc cgtcgaggac tagcggcgcc ggcgatgggg
     2641 ccccacgtgc ggcggcgccg cgctagagga gcttgtgatc gacggcaaag cgggtggcgg
     2701 cggcgcgcga gggcacgtcg agtttgctgt agatactgcg caggtggacg ttgacggtgt
     2761 gcgggctgag cgccagttcg cgggcgacct gcacattggt gagcccctga gccaccagcc
     2821 gtagcaccgc cacttcgcgc gccgtcagcc cggcgggata ttccccacca gggcgttccg
     2881 ccgctggggc aggtgcgccc gaaaccgggg ccaccgcgcc ggcagtggtt cctgccgagt
     2941 ccaatgcgtc cgctacggcg gcagccgagt cgagcgcccg cccggcggcc caggcggcgg
     3001 cccacgtcgc ggtatccact tgcgcccggg ttgccgcgac agcctgatca taggcggtgc
     3061 gggcgacgag cggcatgggc acgccacgcg cctcgcgcaa cgcttccgcc gcaccccaca
     3121 accgcgcagc ctcggctagc cggccctggc gccccgccag ccaggccgcg cattccaggc
     3181 cctgggcgat ccccgccggc gtgccggcca cctgccagat ttccaggctc tccgccagcc
     3241 aggcctgggc gctgcgatag tcgtccttgt cggcggccag gacggccagg tcccgcaatg
     3301 tgagcgcgct gctgttgatg tcaccgacat cgcgatagag caccaggctt tcgtccagca
     3361 gcgcctgcgc ctcctcggta ctaccctgag cctgcgccac gccggccagg tgttgcaacg
     3421 cggtggcgat cccccagcgg tcgccgaggg cgcggcggaa ggccaggctt tgttccagca
     3481 gggcgcgggc gcgcggcaaa tcgccctggc tgcgcaaaac catgcccagg ttgtcgagca
     3541 ccacggcact accccagcgg tcgccgagcg cccggtccaa cgcgaggctc tcttcgagga
     3601 gccgccgggc ctcggcgtac gcggcctgat tccaggccac cacgccgaga ttcaacaggg
     3661 cattggcgac gccccattga tcgcccagac ttcgccgcag cgttaagctc tccgtatgga
     3721 gtgtgcgcgc ctgcggaaaa tcgccctggc tacgtgccac caggcccagc gtgttgaggg
     3781 cattggcctg tcctgccgga tcgttgagtg cccgatgcag cgccaggctc tcctggctat
     3841 acgcggtggc ggtgccgtaa tcgccctgac cccaggcaag caccccggca ccatgcagag
     3901 cacgggcgcg ggcggcggtc agagtcgcgt cttccggggc gggttctact gcgccggcct
     3961 ggctcagcac cgcgctaagt tgcgcccggc cctcgctata atacccccgg atataccaaa
     4021 accgccacag cgccccggcc agccgcaggc cgctctcgat ttgcttctgg ttctgggtcc
     4081 aggccagagc actgcgcaaa ttgtcgtgtt cggcgtcgag ccgcgccaac cagcgctcct
     4141 gcggggcgcc tttcagcaag ggctcggcct cctcggcgag cgccagaaag tagccggcat
     4201 gctgagtctg cacggcgtcg ccctcgccac tcgcagccaa ccgcgcccag gcatgttcct
     4261 gaatcgtctc cagcatacta aagcggg
//
LOCUS       DASUYC010000496         3478 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_350167,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000496 DASUYC010000000
VERSION     DASUYC010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350167"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VF276_08155"
     CDS             <1..234
                     /locus_tag="VF276_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606871.1"
                     /translation="YRRIRAARPSQGPDLPIMEAAGADQPSLPQVVHSLKQMRGAVAT
                     LQRELQRQIQPLEQATTGPRPALRPPDALGGDR"
     gene            437..754
                     /locus_tag="VF276_08160"
     CDS             437..754
                     /locus_tag="VF276_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606872.1"
                     /translation="MDPEDTDSHSFIIRIWLEETAEEGQPAVWRGHITHVPSGRRRYL
                     QEFDALVSFIAPYLEEMRMPVDGGDRRETGGVDTSQPRAPRAGVHKRLMAMMVHIMRR
                     LQG"
     gene            751..1356
                     /locus_tag="VF276_08165"
     CDS             751..1356
                     /locus_tag="VF276_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606873.1"
                     /translation="MNDITYTLSVKVANPVGPTFSATETLKVEAYDRIDVEIRMGKSV
                     TVRIPQNDKRELQMLLIQRTPLDPKSDAATNLGKLTYQVGNAGCWIPLDSTASHFAFG
                     AGFVQLAAQVPADITFKNEQDQTVTVTIFIARKASTDATPSPPATPPPPATPPAPPAP
                     PAPPAPPATGAQPPQGGTTTTLPGGATTPSSTSGGPSSNCP"
     gene            1431..2054
                     /locus_tag="VF276_08170"
     CDS             1431..2054
                     /locus_tag="VF276_08170"
                     /inference="COORDINATES: protein motif:HMM:NF025431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HEX6606874.1"
                     /translation="MSSYQVLAATSEALRDILWDAYNADTVLRPIVGNKQAIVFSNPT
                     ETARDSANRISLWLYQVTENEFLKNQPATRGNSHQSLQETPLALNLFFLVTPFGPSGG
                     ADHLLLGLTMQALYDNAIIRLPDSAGGQGEELRVIFSRLSLEELTLIWQALREPYRLS
                     VCYQVRVTRIDSRRTMQHARAVTVHNDLGIKPVSNESDANELPDRTL"
     gene            2029..3036
                     /locus_tag="VF276_08175"
     CDS             2029..3036
                     /locus_tag="VF276_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606875.1"
                     /translation="MNYPTERFSLTPTLVFSVGDGFARDRAGGQSPARVIGPLQFWLA
                     ARVAPAPREDFATPWEMVMQINRSGYYLFFGLVRWPDGGQRRITLDPGRYLVRITSPL
                     YQPVTQAVSVPMGNLNYPDLTSADPDQRDPMRQYSFSLEPGATYPFPNTVTFRPELVG
                     SGCAEDTAAGRLAPTLLRGSLHSPDGRPIAGARVRVTGRSNTYTTGDSGDWVLWFADT
                     RAIGPVPVHVDFPDGTSIDVSNVCVVRWRETSLRETALRGWVLRSGRGVAGAVITVAG
                     QPGAATSSRDGGWTYYFALNQPDATVDLTATLPDGTSLTIPNVPVRQRATVLAPAFLV
                     L"
     gene            3057..>3478
                     /locus_tag="VF276_08180"
     CDS             3057..>3478
                     /locus_tag="VF276_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606876.1"
                     /translation="MPEYLSPGVYIEEQTTGPRPIEGVSTSTAAFIGPTERGPEHPRL
                     VLSWLEFQRWYGTYLTNSVMPHAVQGFFDNGGQRLFIARVVSETAPLPTTRTVGNLVL
                     MAVGRGAWGNNILVRIEDATHRDEDAGRPWFKLTLLYYR"
BASE COUNT          604 a         1195 c         1127 g          552 t
ORIGIN      
        1 taccgccgga ttcgggcagc gcgcccgtca caaggacccg atctgcccat catggaggcc
       61 gcaggggcag atcagccgtc gctgccgcag gtggtgcaca gtctcaagca gatgcgcggc
      121 gcggtcgcca ccctgcaacg cgagttgcag cggcagatcc agccgctgga acaggccacg
      181 accgggccgc gcccggcgct gcgccccccg gatgccctgg gcggagatcg ctaacgtggc
      241 cccacccgtt tttgcacggc ccctagacgc cgccacggac gcctggcaga cgcctatcca
      301 gataggcggt tgctatactc agtgcacact ctacggccgt cggacgagaa actcgataga
      361 tgcgcccgct gccgcggggg gctgtcgcaa ccgggcgtct gccgggttcg cgccgagcat
      421 tagcgggtag cggcagatgg acccagagga caccgatagc cactcgttca tcatccggat
      481 ctggctcgag gaaaccgctg aggaagggca gccggctgtc tggcgcgggc atatcaccca
      541 tgtgcctagc gggcgccggc ggtatttgca ggagttcgac gccctggtca gcttcatcgc
      601 gccctacttg gaggagatgc ggatgcctgt ggacggcggg gatcggcggg agaccggcgg
      661 cgtggacacg agccaacccc gcgccccgcg cgctggtgtt cacaagcggt tgatggcaat
      721 gatggttcat atcatgagga gattacaggg atgaacgaca taacttacac gctgtcggtc
      781 aaggtagcga acccggtcgg ccccaccttt tcggccaccg agacgctgaa ggtcgaggca
      841 tacgaccgga tcgatgtcga gatccgcatg gggaagtcag tgacggtccg catcccgcag
      901 aacgataagc gggagctgca aatgctgctc atccagcgga cgccgctcga ccctaaaagc
      961 gacgccgcca cgaatctcgg caagctgacc taccaggtcg gtaatgctgg gtgctggata
     1021 cccctggact cgacggcgag ccattttgcc ttcggcgccg ggtttgtgca attagctgcc
     1081 caagtgcccg ccgatatcac ctttaagaac gagcaagacc agaccgtcac cgtgacgatc
     1141 tttatcgccc gcaaggcatc gacagacgcc accccctcgc ccccggcgac cccgccgccc
     1201 ccggcgaccc cgcccgcccc gccggcccca cccgccccgc cggcgccacc tgccacgggc
     1261 gcgcagccgc cgcagggtgg cacgacgacc actctgccgg gcggcgctac gactccgagc
     1321 agcacgtcgg gcggcccgag cagcaactgc ccgtgacgtt ggtgatgccg tcgccgaacg
     1381 ggcggatcgc tgtaccggcg acatcatgtc tgcgctaaag tggggaacct atgagcagct
     1441 accaggtgct ggccgccacc agcgaggccc tgcgcgatat cctctgggat gcctacaacg
     1501 ccgacaccgt gctccggccc attgtgggca acaagcaggc gatcgtcttt agcaatccca
     1561 ccgagaccgc gcgcgactcg gcaaaccgga tctccctctg gctctaccag gtcacggaga
     1621 acgagtttct gaagaatcag ccggcgacgc gtggaaacag ccaccagtcg ctgcaagaga
     1681 cgcccctggc gctgaacctg ttctttctgg tcaccccgtt cggcccttcg ggcggcgcgg
     1741 accacctgct gctcggcctg accatgcagg cgctctacga taacgccatt atccggctgc
     1801 ccgattccgc gggcgggcag ggcgaggagt tacgggtgat cttcagccgg ctgtcgctgg
     1861 aggagttgac gctcatctgg caggccctgc gcgaacctta ccggctgtcc gtctgctatc
     1921 aagtgcgcgt gacgcggatc gattcccgcc ggaccatgca gcacgcgcgg gcagtcacgg
     1981 tccataacga cctgggcatc aagccggtca gcaatgagag cgacgcaaat gaactacccg
     2041 accgaacgct ttagtctcac gcccacgctg gtcttcagtg tgggggatgg atttgcccgc
     2101 gatcgggcgg gggggcagag tccggcccgc gtcattgggc cgctacagtt ctggctggcc
     2161 gcccgcgtcg cgccggcccc gcgcgaggat ttcgccacgc cctgggagat ggtcatgcag
     2221 atcaaccgca gcggctatta cctgttcttt ggcctggtgc gatggcccga cggcgggcag
     2281 cggcgcatta ccctcgatcc cggccgctat ctggtccgga tcacgagccc gctctaccag
     2341 ccggtcaccc aggcggtcag cgtgccgatg ggcaacctga actacccgga cctcaccagc
     2401 gcggacccgg atcagcgcga tccgatgcgc cagtattcgt ttagcctgga accgggggcc
     2461 acctatccgt tcccaaacac cgtgaccttc cgcccggaac tggtgggcag tgggtgtgcg
     2521 gaagacaccg ccgcgggacg cctggccccg acgctgctgc gtggcagcct gcacagtccc
     2581 gacggccggc ccattgccgg cgcccgcgtg cgcgtcaccg gccggtcgaa cacctacacg
     2641 accggcgaca gcggagactg ggtgctctgg ttcgccgata cacgggccat cgggccggtc
     2701 cccgtccatg tggactttcc cgacggaacc agcatcgacg tgtccaacgt gtgcgtggtc
     2761 cgctggcgcg agaccagctt gcgcgagacg gccctgcggg gttgggtctt gcggagcggc
     2821 cggggtgtgg ccggggcggt gatcacggtg gccgggcaac cgggcgccgc gaccagcagc
     2881 cgcgatggcg gctggaccta ctacttcgcg ctgaaccagc cggacgccac ggtggacctg
     2941 acggccacgc tgccggacgg caccagcctg accataccga atgtgccggt gcggcagcgg
     3001 gccaccgtgc tcgccccggc gttcctcgtt ctctgagccg tataaggagg cttcgcatgc
     3061 ctgaatatct ctcccctgga gtctatatcg aggagcaaac caccggaccg cggcccatcg
     3121 aaggggtcag caccagcacc gcagccttca tcgggccgac ggagcgcggt cccgaacatc
     3181 cgcggctggt gttgagctgg ctggagttcc aacgctggta tggcacgtat ctgaccaact
     3241 ccgtcatgcc gcatgccgtc cagggcttct tcgacaacgg cgggcagcgc ctgtttatcg
     3301 cgcgggtggt ctcggaaacc gcgcccctgc cgaccacccg caccgtgggc aacctggtgc
     3361 tgatggctgt cgggcggggc gcctggggca ataacatcct tgtgcgcatc gaagatgcca
     3421 cccaccggga cgaggacgcg ggccgcccct ggttcaagct gacgctgctc tactaccg
//
LOCUS       DASUYC010000497         2416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1203985, whole genome shotgun sequence.
ACCESSION   DASUYC010000497 DASUYC010000000
VERSION     DASUYC010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1203985"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            208..1746
                     /locus_tag="VF276_08185"
     CDS             208..1746
                     /locus_tag="VF276_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HEX6606877.1"
                     /translation="MTTLSLQTLPATRLDPADDVAIALRPLRAGQTITVAGQRITLQA
                     AIPPGHKFALRAVAAGAPVRRYGQIIGTATAPIAPGEHVHTQNLHVAALHLDYAVGAD
                     AQPVAFAPAAERATFLGYRRADGRVGTRNIIAVATTVNCSAHTARRIAARARAELLPR
                     FPYVDNVVALTHGAGCGTRAGSAELDVLQATIAGHVRHPNVAGAVIVGLGCEANQFAD
                     LSARQSLDDSAPRFLGIQDEGGVAATVAQGLAALEPLLAAADQARREPVPASELVLAL
                     QCGGSDGFSGITANPALGLAADWLVAQGGTVVLAETPEIYGAEHLLTRRAAAPEVAAQ
                     LLERIGWWENYAAMHGFELDNNPTPGNKAGGLTTIHEKSLGAVAKAGSTPLNGVYRYA
                     ERIDRRGFVFMDTPGYDPVSVTGQVAGGCNLVAFTTGRGSCFGCAVAPSIKIATNSVT
                     YEHMGDDMDVNAGVVLDGVPLATVAREIADLLLAVASGQASKSEAQGIGEEEFDPWIL
                     GATL"
     gene            1804..>2416
                     /locus_tag="VF276_08190"
     CDS             1804..>2416
                     /locus_tag="VF276_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606878.1"
                     /translation="MPAQCTMRDATTDSFLAAHAAPADATGEIVRWIGAVGLAGAPQV
                     RHHCALLGLRVPGDPVLRRLVAAGYLQLVKLPIPFLRYRGVYLPAAQGYALLDPEAGY
                     PRLRGLPIPGYRALILAQEARLHIEQQLAAQGGAILHWQPEPFPPGGRGTTLGGAHVL
                     LRPGPNEDPRVVHVVVHDPASAPPGDLAARAAAYHQEAHPVFWV"
BASE COUNT          342 a          882 c          860 g          332 t
ORIGIN      
        1 ggcacaagac gagactcttc gctgcgctca gagtgacaag ctgtcggtgt cattctgagc
       61 ggagcgaaga atctccgtct cagtcgagca caagacgaga ctcttcgctg cgctcagagt
      121 gacaaagggc ttgggacaga ctaccgggag ccgcggcgtg cacagacagg aggacggctc
      181 gatgctcaca ttccaggcag gcgccccatg acgaccctta gcctccagac gctgcccgcc
      241 acccggctcg acccggcgga cgacgtggcg attgccctgc gcccgctgcg ggccgggcag
      301 accatcactg tggccgggca gcgcatcacg ctccaggcgg ccatcccgcc cggccacaag
      361 ttcgcgctgc gggccgtcgc ggcgggcgcc cccgtgcgcc gctacgggca gatcatcggc
      421 acggccaccg cgcccatcgc gccgggcgag catgtgcaca cgcagaacct gcatgtggcc
      481 gcgctacacc tcgactacgc ggtgggcgcc gatgcgcagc cggtcgcctt cgcgcccgca
      541 gccgagcgcg ccacgttcct gggctaccgc cgcgccgacg gccgggtggg cacgcgcaac
      601 atcatcgccg tggcgacgac cgtgaactgc tcagcgcata ccgcgcggcg tatcgcggcg
      661 cgggcgcggg ccgagttgct gccgcgcttc ccctacgtgg acaacgtggt ggcgctgacg
      721 cacggggcgg gctgtggcac gcgcgccggc agcgccgaac tcgacgtgtt gcaggcgacc
      781 atcgccgggc atgtgcgcca ccccaacgtc gcgggcgccg tgatcgtggg cctcggctgc
      841 gaggccaacc agttcgccga cctcagcgcc cgccagagcc tcgacgactc cgcgccgcgc
      901 ttcctgggca tccaggacga gggcggggtg gcggcgacgg tggcgcaggg gctggccgcc
      961 ctggaaccgc tgctggccgc ggcggaccag gcgcgccggg agccggtgcc ggcatcggag
     1021 ctggtgctgg cgttgcaatg cggcggcagc gacggcttct ccggcatcac ggccaacccg
     1081 gcgctgggcc tggccgccga ctggctggtg gcccaggggg gcacggtggt gctggccgag
     1141 acgcccgaaa tctacggggc cgagcacctg ctgacccgcc gcgccgccgc gccggaggtg
     1201 gccgcgcagt tgctggagcg catcggctgg tgggaaaact acgcggccat gcacgggttt
     1261 gaactcgaca acaaccccac gcccggcaac aaggcgggcg gcctcaccac gatccacgag
     1321 aaatcgctgg gcgccgtcgc caaggcgggc agcacgccgc tgaacggcgt ctaccgctac
     1381 gcggagcgca tcgaccggcg cggcttcgtg ttcatggaca cgcccggcta cgacccggtg
     1441 tcggtcacgg gacaggtggc ggggggttgc aacctggtgg ccttcaccac cgggcgcggc
     1501 agttgcttcg gctgtgccgt ggcgcccagc atcaagatcg ccaccaacag cgtgacctat
     1561 gagcacatgg gcgacgatat ggatgtgaac gccggggtgg tgctcgacgg cgtgcccctg
     1621 gcgacggtgg cccgcgagat cgccgatctg ctgctggccg tcgcctccgg gcaggccagc
     1681 aagagcgaag cccagggcat tggcgaggag gagttcgatc cgtggatcct cggcgccacc
     1741 ctgtgatccc ggcgcgggtg ggaaccaggc cggtttcaaa ccggccccta caggcggccc
     1801 ctgttgccgg cccaatgcac catgcgcgac gcgacgactg actccttcct ggccgcgcat
     1861 gccgcgccgg ctgacgccac gggcgagatc gtgcgctgga tcggcgcggt cggtctggcc
     1921 ggtgccccgc aggtgcgcca ccactgcgcg ctgctgggcc tgcgcgtgcc gggcgatccc
     1981 gtgctgcgcc gcctcgtcgc cgccgggtac ctgcaactgg tcaagctgcc gatcccgttc
     2041 ctgcgctatc gcggggtcta tctgcccgcc gcccagggct atgcgctgct cgatccggag
     2101 gccggctatc cccgcctgcg cgggctgccc atccccggct atcgcgcgct gattctggcg
     2161 caagaggccc gcctgcacat cgagcaacag ttggccgccc agggcggcgc catcctgcac
     2221 tggcagcccg agccgttccc gccggggggg cgcggcacca cgctgggcgg cgcgcacgtg
     2281 ctgctgcgtc ccggccccaa cgaagacccg cgcgtggtgc atgttgtcgt ccatgacccg
     2341 gcgtcggcgc cgcccggcga tctcgcggcc cgcgctgccg cctatcacca ggaggcgcac
     2401 cccgtcttct gggtct
//
LOCUS       DASUYC010000498         2012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1047800, whole genome shotgun sequence.
ACCESSION   DASUYC010000498 DASUYC010000000
VERSION     DASUYC010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1047800"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /gene="hppD"
                     /locus_tag="VF276_08195"
     CDS             <1..739
                     /gene="hppD"
                     /locus_tag="VF276_08195"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018673705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEX6606879.1"
                     /translation="DDHGTVKRAAIATYGDTIHSLVERDYHGTFMPGFQPRAIKGKGV
                     GIRVVDHIVGNVELGHMDEWVAFYANVMGFEQLVHFDADQISTEYSALMSKVVQDGTG
                     KIKFPINEPAQGKKKSQIDEYLEYYNGPGAQHIAVATGDILATVQHLEANGCEFLKTP
                     HTYYQDLPERIGKIKEDLEQIEAHSILADRDEDGYLLQIFSKPVQDRPTLFYELIERH
                     GCRGFGEGNFKALFEAIEREQALRGNL"
     gene            752..1939
                     /locus_tag="VF276_08200"
     CDS             752..1939
                     /locus_tag="VF276_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003386699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEX6606880.1"
                     /translation="MPFYHRVGEVPPKRHTQFRRPDGELYTEELLGNEGFSGLASLLY
                     HYYPPVRVTGITPCPLAPAEEWREPVQRHYHLRTKAAEPGGDPITSRRVLMFNDDVSL
                     GIARPTAPMDGYYYKNGEADELYFIHEGTGTFETMYGALPYHEGDYVVIPHGTIYRVV
                     MESTPQRWLVIESRGRIETPKRYRNEFGQLLEHSPYCERDIRVPAAPLALLDAPGAEV
                     RIKANGRLSAYHYDHHPFDVVGWDGYLFPWILNIADFEPITGRIHQPPPTHQTFQGRN
                     FVVCSFVPRKYDYHPLSIPAPYNHSNIDSEEVLYYVNGNFMSRRGIDVASFTLHPHGI
                     PHGPHPGAAEASIGKEQTDELAVMVDTFRPLKLTRWAKEYDDAQYPFSWVDPSPAPGG
                     PTK"
BASE COUNT          396 a          703 c          599 g          314 t
ORIGIN      
        1 cgacgaccac ggcaccgtca agcgcgccgc catcgccacc tatggcgaca ccatccacag
       61 cctggtcgag cgcgattatc atggcacgtt catgcccggc ttccagccgc gggcgatcaa
      121 gggcaagggc gtcggcatcc gcgtcgtgga ccatatcgtc ggcaacgtcg aactgggcca
      181 catggacgag tgggtggcct tctacgccaa cgtgatgggc ttcgagcaac tggtccactt
      241 cgacgccgac cagatctcca ccgaatactc ggcgctcatg tcgaaggtgg tccaggacgg
      301 caccggcaag atcaagttcc cgatcaacga gccggcccag ggcaagaaga agtcgcagat
      361 cgacgagtat ctggagtatt acaacggccc cggcgcccag cacatcgccg tcgccaccgg
      421 cgacatcctg gcgaccgtgc agcacctgga ggccaacggc tgcgagttcc tgaagactcc
      481 gcacacctat taccaggatc tgcccgagcg catcggcaag atcaaggaag acctggaaca
      541 gatcgaagcc cacagcatcc tggccgaccg cgacgaggac ggctacctgt tgcagatctt
      601 cagcaagccg gtgcaagacc ggcccaccct gttctatgaa ctgatcgagc gtcacggctg
      661 ccgcgggttt ggcgagggca atttcaaggc cctgttcgag gccatcgagc gcgagcaagc
      721 gctgcgcggc aacctgtagg agggccacgc catgccgttc tatcatcgag tgggcgaggt
      781 gccgcccaag cggcacacgc aattccgccg ccccgacggc gaactctata ccgaagaact
      841 gctgggcaac gagggctttt ccggcctggc ctcgctgctc taccactact acccgccggt
      901 gcgtgtgact ggcatcacgc cctgcccgct ggcgccggcg gaggagtggc gcgaaccggt
      961 gcagcgccac taccacctgc gcaccaaggc cgccgagccg ggcggcgacc cgatcaccag
     1021 ccgccgcgtg ttgatgttca acgacgacgt gtcgctcggc atcgcccgcc cgaccgcgcc
     1081 gatggacggc tactactaca agaacggcga ggccgacgaa ctctacttca tccacgaagg
     1141 cacgggcacc ttcgagacca tgtacggcgc gctgccctat cacgaaggcg actacgtggt
     1201 catcccccac ggcacgatct accgggtggt gatggagtcg acgccgcagc gctggctggt
     1261 gatcgagtcg cgcgggcgca tcgagacgcc gaagcgctac cgcaacgagt tcggccagtt
     1321 gctggagcac tcgccctact gcgaacgcga catccgcgtg cccgccgcgc cgctggcgtt
     1381 gctcgacgcg cccggcgccg aggtgcgcat caaggccaac gggcggctca gcgcttacca
     1441 ctacgaccac caccccttcg acgtggtcgg ctgggacggc tatctcttcc cgtggatcct
     1501 caacatcgct gacttcgagc cgatcaccgg gcgcatccac cagccgccgc ccacgcacca
     1561 gacgttccag ggccgcaact ttgtggtctg ctcgttcgta ccgcgcaagt acgactacca
     1621 cccgctgtcg atccccgcgc cctacaacca cagcaatatc gacagcgagg aagtgcttta
     1681 ctacgtgaac ggcaacttca tgtcgcgccg cggcatcgac gtggcctcgt tcacgctcca
     1741 tccgcacggc atcccgcacg gcccgcaccc cggcgcggcg gaagcatcca tcggtaagga
     1801 gcagacggac gaactggcgg ttatggtcga caccttccgc ccgctcaagc tgacccgctg
     1861 ggcgaaggaa tacgacgacg cgcagtaccc gtttagctgg gtcgatccat ctccggcccc
     1921 cggcgggcca acgaaatagc tatcagcgat cagccgtcag ctatcaggtt agtacagggt
     1981 tatcgtctta gtgtctagct gccgccaagg gc
//
LOCUS       DASUYC010000499         3288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_947583,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000499 DASUYC010000000
VERSION     DASUYC010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947583"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..3165
                     /locus_tag="VF276_08205"
     CDS             142..3165
                     /locus_tag="VF276_08205"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX6606881.1"
                     /translation="MTQTIPQVYGGRLAAPAAGGEYATITLGSPDWYAWLGDNVHFTF
                     AGDLGTFTARKERRARGDEYWYVYRKHGGKLHKAYLGRSADLSPERLYAVTSALTRRL
                     AGDTDVPLRLHNGAHPAPAAASEPGAALLLTKLTVPRPPAGLIARPRLSARLAASAPR
                     PLTLVAAPAGFGKTTLVSEWARQCELPIAWVSLDPADSDPARFWRYVIAALDGVHPGV
                     GVGASAVLHAPGPVPTPALLTALLNALAAAPGDLLLVLDDYHLIDTPQIHDGMAFLLD
                     HLPATLRLVIAGRGDPPLPLARLRARGQVSELRAADLRFTLDEAAAFFRRTLGLDLSE
                     ADLAALDARTEGWIAGLQLAALSLRDAPAGPDVARLVTGDDRHILDYLVAEVLAQQPP
                     AVQDFLLQTAILPRLTGPLCDAVTDRPGGQAMLERLEAANLFLIPLDNTRTWYRYHHL
                     FAEVLHRRLRQDAPGSIAILHARASAWHAQQGQTNEAIEHALAAEDWPCLLDLIESLD
                     RDLAARGEQATLRRWLSAVPDAILAGRPGLCLRYAWALLFHQHISACERPLAVAEQAW
                     RSSGDDARLGEVLILRSALASFQGDPAGALTCAREALDRLPAADLFQRNIAYAYVFHA
                     YRAIGDLAGASAALAGQRALGQVVAGTPVPAIAAGIPLDVEISAGHMAVAAGRLREAA
                     AAYRLAIRLAGRSQLRARMGAHWHLSQVLREWNDLDAAEEHARQGTAICAQIGETLSL
                     PEGHLLIARILAARGDYAGATQELAQARAGARLVGRDQLGQVLETEAARLWLRQARLA
                     EAGRWAAGCGLPLDALPPYERQFEYLTLVRVRLAQHLPGNAPRVLARLIEAAEAAGRI
                     REVMGCLVLLALAHAARGATDQALPPLERALALAEPEGYIRLFVDEGAPLAALLDHLA
                     AQRPPTAYVRTLRAAFAADTAPAAAPVAYTTPLSPREAGVLRLLGQGASNDQIAADLT
                     IAVDTVKKHVSHIYTKLDVTSRTQAVARARQLGLL"
BASE COUNT          429 a         1363 c         1067 g          429 t
ORIGIN      
        1 cagagtccaa ctaaactccc ccttcccgcg gcgggaaggg ggacgggggg ttaggttagc
       61 cgggccactt gcgcagttac ccaacaccgg gtatactgcg cgaagacacc ggaccaggga
      121 caggggacag gaggaaccag cgtgacgcaa actattccac aagtttacgg cggcaggctg
      181 gccgccccgg cggccggggg cgagtacgcg accatcacgc tcggctcgcc cgactggtac
      241 gcctggctgg gcgataacgt ccacttcacg tttgccggcg atctggggac gttcaccgcg
      301 cgcaaggagc gccgcgcccg cggcgacgaa tactggtacg tctaccgcaa gcacggcggc
      361 aagctgcata aagcctacct gggccgcagc gccgatctct cgcccgaacg gctctacgcc
      421 gtcacctcgg cgctcacccg gcggctggcc ggagatacgg atgtacccct ccggttacac
      481 aacggcgctc atcccgcgcc cgccgccgca agcgaacccg gcgcagcgct gctgctgacc
      541 aagctcaccg tgccgcgccc gcccgccggc ctgatcgcgc gcccgcgcct gagcgcccgg
      601 ctggccgcgt cggcgccccg cccgctgacc ctggtcgccg cgcccgccgg cttcggcaag
      661 accacgctgg tcagcgaatg ggcgcgccag tgcgagttac ccatcgcctg ggtctcgctc
      721 gaccccgccg actccgaccc ggcccgcttc tggcgctacg tcatcgccgc cctcgacggc
      781 gtgcatccag gcgtcggcgt cggggccagc gccgtgttgc acgcgcccgg ccccgtgccc
      841 acgcccgcgc tgctcaccgc cctgctcaac gccctggccg ccgcccccgg cgacctgctg
      901 ctcgtgctcg acgattacca cctgatcgac acgccgcaga tccacgacgg catggccttc
      961 ctgctcgacc acctgcccgc cacgctacgg ctggtcatcg ccgggcgggg cgatccgccg
     1021 ctgccgctgg cccgcctgcg cgcccgcggc caggtcagcg aactgcgcgc cgccgatctg
     1081 cgcttcaccc tggacgaagc cgccgcgttt ttccgccgca ccctggggct ggacctgagc
     1141 gaagccgacc tcgccgcgct cgacgcccgc accgagggct ggatcgccgg gctgcaactg
     1201 gccgcgctct ccctgcgcga cgcgcccgcg ggccccgatg tcgcccgcct ggtcaccggc
     1261 gacgaccgcc acatcctcga ctacctggtg gccgaggtgc tggcgcagca gccgcccgcc
     1321 gtgcaggatt ttctgctgca aacggccatc ctgccccgcc tgaccgggcc gctgtgcgac
     1381 gccgtgaccg accggcccgg cggccaggcc atgctggaac ggctggaggc cgccaacctg
     1441 ttcctgatcc cgctcgacaa cacccgcacc tggtaccgct atcatcatct cttcgccgag
     1501 gtgctgcacc gccgcttgcg ccaggacgcg cccggcagca tcgccatcct gcacgcccgc
     1561 gcgagcgcct ggcacgccca acaaggccag accaacgagg ccatcgagca cgccctggcc
     1621 gctgaggact ggccgtgcct ccttgacctg atcgaatcct tggaccggga cctggccgcg
     1681 cgcggcgagc aggcgacttt gcgccgctgg ctgtccgccg tacccgacgc catcctggcg
     1741 ggccgccccg gcctctgcct gcgctacgcc tgggccctgc tcttccacca gcacatcagc
     1801 gcctgcgagc gcccgctggc cgtcgccgag caggcctggc gcagcagtgg cgacgacgcc
     1861 cgcctgggcg aggtgctgat cctgcgcagc gccctggcga gtttccaggg cgaccctgcc
     1921 ggcgccctga cctgcgcccg cgaagccctg gaccgcctgc ccgccgccga cctgttccag
     1981 cgcaacatcg cctatgcgta cgtgtttcat gcctaccggg cgatcggcga cctggcgggg
     2041 gcctcggcgg ccctggccgg gcagcgcgcg ctgggccagg ttgtggccgg gacgccggtc
     2101 ccggccatcg ccgccggcat tccgctcgac gtcgagatca gcgcgggcca tatggcggtc
     2161 gccgccgggc ggctgcgcga ggccgccgct gcctaccggc tcgccatccg cctggccggg
     2221 cgcagccagc ttcgcgcccg catgggcgcc cactggcatc tctcccaggt cttgcgcgag
     2281 tggaacgacc tcgatgccgc tgaggagcac gcccgccagg gcaccgccat ctgcgcccag
     2341 atcggcgaga cgctctcgct gcccgagggc cacctgctga tcgcccgcat cctggccgcc
     2401 cgcggcgact atgccggcgc gacccaggaa ctggcccagg cgcgcgcggg cgcgcgcctg
     2461 gtgggccgcg accagcttgg gcaggtgctg gagacggaag ccgcccgcct ctggctgcgc
     2521 caggcgcgac tggccgaagc cgggcgctgg gccgccggct gcggtctacc gctcgatgcg
     2581 ctcccgccct atgagcgcca gttcgagtat ctcaccctgg tgcgcgtgcg cctggcccag
     2641 cacctgcccg gcaacgcgcc gcgcgtgctg gcccgcttga tcgaggccgc cgaggccgcg
     2701 ggccgcatcc gcgaggtcat gggctgcctg gtgctgctcg cgctggccca cgcggcgcgc
     2761 ggcgccacgg atcaggccct gcccccgctc gaacgcgccc tggccctggc cgagcccgag
     2821 ggctatatcc gcctcttcgt ggacgagggc gcgccgctgg ccgccctgct cgaccacctg
     2881 gccgcccaac ggcccccgac ggcctacgtc cgcaccctgc gcgccgcctt cgccgccgac
     2941 acggcccccg ccgccgcgcc cgtcgcctat accacgccgc tcagcccgcg cgaagccggc
     3001 gtgctgcgcc tgctcggcca gggcgcctcc aacgaccaga tcgccgccga cctcaccatc
     3061 gccgtggaca cggtgaaaaa gcacgtcagc cacatctaca ccaagctcga cgtgaccagc
     3121 cgcacccagg ccgtcgcccg cgcccgccaa ctcggcctgc tctgacgcgg gcctaacccc
     3181 cccgccccct tcccggcgcg ggaagacgcg ggcctaaccc ccccggcccc cttcccggcg
     3241 cgggaagggg gagagcagtg ggcctaaccc ccccgccccc ttcccggc
//
LOCUS       DASUYC010000500         3154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1408394, whole genome shotgun sequence.
ACCESSION   DASUYC010000500 DASUYC010000000
VERSION     DASUYC010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408394"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1273
                     /locus_tag="VF276_08210"
     CDS             <1..1273
                     /locus_tag="VF276_08210"
                     /inference="COORDINATES: protein motif:HMM:NF039189.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5671 domain-containing protein"
                     /protein_id="HEX6606882.1"
                     /translation="RWYYYLINFGALSFALSGIAGLARYVWDLITSGGSPAIVETTGL
                     PGEFTTNTGWVIAGMAFWLLHWLPVQRLTAVSDDEQHAVLRKVYLYGIIFETVAVTLT
                     SVALFLYNLLRYLLGTNPLADSDKSLLTAAGEPLLTALVYGLFWVYHRRVLAWDAGLV
                     GEEPRQAGIRRLYHYLVALIALAVLAGGVTSMLRLLIDLWLGGSATSALNRQTWGDQI
                     SLFATLIIVGAPVWYANWARLQRWAMAPDGGADRQAVLRRIYLFLILFAGVITLLVST
                     AWIIYQVLRTLGDSFTGSLISDMSWALGAAVTAAVLLAYHLRVLLGDQRAGAAATQAD
                     RGELVPAPAPPPDDALAVVVAVRSRNPPAVEEALGKLQDMLPAGSVVEILPAGNVTAG
                     ELRAWLAHHPPPPEPPALQSDSPPGSRPIPA"
     gene            1873..2625
                     /locus_tag="VF276_08215"
     CDS             1873..2625
                     /locus_tag="VF276_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606883.1"
                     /translation="MLSNRRIWRAPILAAAICTGLAFAGAPAAYAHAPSPEAGAPVAA
                     SARTGIARGAARPATTPAPAPAAPARPTAAAKKTTTAKKAVPAKTPTVKKAATTTKKA
                     TPAKTTTVKKAATTKSSSASTKAKSTASAKSPAPAKSTAKVERGIAMHYYPGLFATVA
                     RNRGMTLRKDVDGYASRQSCAELGRVVEARIRNPRTGAWGSWKRYQIVDCSARKHLAY
                     QRSIGLILEVDYASAAKAGFVYRGRSAVEVKR"
     gene            complement(2703..>3154)
                     /locus_tag="VF276_08220"
     CDS             complement(2703..>3154)
                     /locus_tag="VF276_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606884.1"
                     /translation="PLIETSTGVEGWVVRFPNNLRVKVKTADYFRLHRARFMLSPETV
                     REALVAGTWDAFMIGLPEEFHRESSAIAAAIRALVAAEETRLCALFAEVCAAVPDDSR
                     KTFALYVQAHHRADGAYLFPLWEGRDIGPLLLRKIDLSGCVPQAPAE"
BASE COUNT          514 a         1092 c         1044 g          504 t
ORIGIN      
        1 ccgctggtac tactacctga tcaatttcgg cgccctgtcg tttgccctga gcggcatcgc
       61 gggcctcgcc cgctatgtct gggatctgat caccagcggg ggatcgccgg cgatcgtgga
      121 gaccaccggg ctgcccggcg agtttaccac caacacgggc tgggtcatcg cgggcatggc
      181 gttctggttg ttgcactggc tgccggtgca gcggctgacc gccgtctcgg acgacgagca
      241 acatgccgtg ctgcgcaagg tctacctgta cggcatcatc ttcgagaccg tggcggtcac
      301 gctcaccagc gtcgcgctct tcctctacaa cctgctgcgc tatctgctgg gcaccaaccc
      361 cctggcggat tccgataagt cgttgctcac agccgccggg gaaccgttgc tgacggcgct
      421 ggtgtatggc ctgttctggg tctatcaccg gcgggtcctc gcctgggatg ccgggctggt
      481 gggggaagag ccgcgccagg ccggtatccg ccggctctac cactacctgg tcgcgctgat
      541 cgccctggcc gtcctggccg gcggcgtcac cagcatgttg cggctgctaa tcgacctgtg
      601 gctcggcggc agcgcgacga gcgcgctcaa ccgccagacc tggggcgacc agatcagcct
      661 ctttgccacg ctaatcatcg tgggcgcgcc cgtctggtat gccaactggg cccggctgca
      721 acgctgggcc atggccccgg acggcggcgc ggaccgccag gcggtcctcc ggcggatcta
      781 cttgttcctg atcctgttcg ccggtgtcat caccctgctg gtgagtaccg cgtggatcat
      841 ctaccaggtg ctgcgcacgc tcggcgacag tttcaccggc agcctgatca gcgacatgag
      901 ctgggcgctg ggcgccgccg tcaccgcggc ggtgctgctg gcctaccatc tgcgcgtcct
      961 gctgggcgat cagcgagcgg gggccgccgc aactcaggct gatcggggtg aattggttcc
     1021 ggcgccggcc ccgccacccg atgatgccct ggcggtcgtt gtggccgtgc gcagtcgcaa
     1081 cccgcccgcc gtcgaggagg cgctggggaa gttgcaagac atgctacccg ccgggagtgt
     1141 ggtagagatc ttaccggccg gaaacgtgac ggccggcgaa ctgcgtgcct ggctcgcgca
     1201 ccacccaccg ccgccagagc cccccgcgct ccagtccgat agcccgcccg gcagccggcc
     1261 catcccggct tgagttgccg ggcgcccccg cgtctgccca attaccggcg gttccaggcc
     1321 ggcaaatgcc cgttttccgg ccgtatgcgg taatgatcct gcccgaaaac gggtactttg
     1381 tgctgcccgc aggatcgccg tcagggtggt atggttgcgg taaggatatc cgcccgtttt
     1441 gataccaaaa tgtgacctct taacttgaca agagtatgct atattcttgt tagcaaggtc
     1501 gtcgcagcag accaccccac gccgtggtgt tcatgccccg caaccgcttt gctgggaccg
     1561 tcgagatcag ccgcgccgcc gcatgcccgg ccccccctgc cctacccgcc ggtaggagca
     1621 tggagtggtg tgtggcccca tgcacacagc atgggcgtgg ctggcggatg gaacgagagc
     1681 acacgtgccc tgtccaccgg gaattcctat gatcgcgcga taagttggcg cgaacggaac
     1741 cggtgggcag gattcatccg ggcgcttgcc agacgctggg ctgcaccgac gaacgtcgca
     1801 tgccggcaga ctccaggaga tggggcagcg ccccggtgcg gacgcgtggg gctctctcgt
     1861 gaaggagttc ccgtgttaag taacagacga atatggcgcg cgcccatcct ggcggcggcg
     1921 atctgtacag gcctggcgtt tgccggggca cctgcggcct atgcccacgc cccgtccccg
     1981 gaagccggcg caccagtcgc cgctagcgcg cgcaccggca tcgcgcgcgg ggcagcgcgc
     2041 ccggccacga ccccggcccc cgcccctgcc gccccggcca ggcccacggc cgcggcgaag
     2101 aaaaccacca ctgccaaaaa ggccgtcccg gccaagacgc ccaccgtgaa gaaagccgcc
     2161 acgaccacga aaaaggccac cccggccaag accaccaccg tgaagaaagc cgccacgacc
     2221 aagtcgagta gcgcaagcac gaaggccaag agcaccgcat cggcgaagag tccggctccg
     2281 gccaagagca cggcgaaagt cgaacgcggt atcgccatgc attactatcc gggcctgttt
     2341 gccaccgtgg cccgcaaccg cggcatgacg ctacgcaagg atgtagacgg ctacgccagc
     2401 cggcaaagct gcgccgagct aggccgggtg gtcgaggccc gcattcgcaa tccccgcacc
     2461 ggcgcctggg gatcgtggaa acgctaccag atcgtcgatt gctcggcacg taagcacctc
     2521 gcctatcaac gcagcatcgg cctgatctta gaggtggact acgcgagcgc ggcgaaggcc
     2581 gggtttgtct accgcgggcg cagcgcggtc gaagtcaagc gttagcagag acccggcggc
     2641 gcggcggcgc tccggcacca ggaggccggc ggggtggtcg cgtgaccacc ccgccggtgt
     2701 gtttattcgg cgggcgcctg cggcacgcac ccgctgaggt cgatcttccg cagcagcagc
     2761 gggccgatgt cgcgcccttc ccagagcggg aacagatagg cgccgtcggc gcggtgatgc
     2821 gcctgcacgt agagcgcgaa ggtcttgcgc gagtcgtcgg gcaccgccgc gcacacctcg
     2881 gcgaagagcg cgcacaagcg agtctcttcg gcggctacca gcgcccggat ggccgcggcg
     2941 atggcgctgc tttcgcggtg gaactcctcc ggcagcccga tcatgaaggc gtcccaggtg
     3001 ccggcgacca gggcctcgcg caccgtctcg ggcgacagca tgaagcgggc gcggtgcagc
     3061 cggaagtagt ccgccgtctt caccttgacg cgcaggttgt tcgggaagcg caccacccag
     3121 ccttccacgc ccgtgctcgt ctcgatcagc ggca
//
LOCUS       DASUYC010000501         2680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_24501,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000501 DASUYC010000000
VERSION     DASUYC010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24501"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="VF276_08225"
     CDS             <1..774
                     /locus_tag="VF276_08225"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX6606885.1"
                     /translation="VAVIGANVADQLFQGEDPLDKTIRIDRLAFRVVGVAAPKGGTGF
                     GSLDDGVYIPVTVAQRKLFGGRQAAIAGHNVNTIYVQVDNKDDMVAAQNQITDLLHTR
                     HHLTTSDNDFTVINQADLLNTLNGVVATLTLFLGAIAGISLLVGGIGIMNIMLVSVTE
                     RTREIGIRKAVGAQQGDILRQFLIEAILISVGGGLLGILLGFIIANLVTLTGTLTSIV
                     TPGAALLAVGFSLAVGLFFGIYPARRAARLDPIVALRYE"
     gene            800..1342
                     /locus_tag="VF276_08230"
     CDS             800..1342
                     /locus_tag="VF276_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606886.1"
                     /translation="MRNPFVMLVVGLVIGLIIAAGAFIGGMNMGQAQAQEQQNQFFAQ
                     RGFNPNAAGGTGAGGAGGQFGGAGGTGGAGRTGGAAGRNATFGTIKKIDGNTITLTVP
                     NSEDITVSLPANATIIKTVTGTVSDLKEGQRLTVMGTRSGNNVAATAIQVTDLPAGEN
                     FPGFGGGRNGGARATPTPGQ"
     gene            1364..1735
                     /locus_tag="VF276_08235"
     CDS             1364..1735
                     /locus_tag="VF276_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606887.1"
                     /translation="MRTWVLRIRAADRPIFDALVDGRKTIETRAGATGARSYTRAQPG
                     DTLLFLCGPARLRRIVTRVAVYPSPAALAADEDVRRVLPWITSAADLAALYESFPGYP
                     ERIARHGLVAMELAPPLEDTT"
     gene            1833..2174
                     /locus_tag="VF276_08240"
     CDS             1833..2174
                     /locus_tag="VF276_08240"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX6606888.1"
                     /translation="MPKMVSATEAKNRLGALFNWIQDNQDTVIVESRGEPTAVIMSYS
                     EYEKIQALKEQARRQEIWERMEALRREVRANNPDITTEEQAMEIADQFTREAIASLVK
                     KGKIKFTQPEP"
     gene            2177..2605
                     /locus_tag="VF276_08245"
     CDS             2177..2605
                     /locus_tag="VF276_08245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HEX6606889.1"
                     /translation="MRVLCDTNVFISYLLTPERAGVVAAIVQAAFQDRFTLILPEDVV
                     QELITVTRAKSYLSSRISLQTVTAFVQVLQDLAEYLPAVIEAIPPVSRDPKDDYLLVL
                     ALIGRADYLVTGDEDLLSLKQVETVAILRPSEFAALLAET"
BASE COUNT          541 a          800 c          844 g          495 t
ORIGIN      
        1 gtcgccgtga tcggcgccaa cgtcgccgat cagctcttcc agggggaaga tcccctcgat
       61 aagacgattc gcatcgaccg cctggccttc cgtgttgtgg gcgtggcggc gccgaaaggc
      121 ggcaccggct tcggttcgct ggatgacggc gtctatatcc cggtcacggt ggcccagcgc
      181 aagctgttcg gcggccggca agccgccatc gccgggcaca acgtgaacac aatctatgtc
      241 caggttgata acaaggacga catggtggcc gcgcagaacc agatcaccga cctgttgcac
      301 acgcgccacc accttacgac ctccgacaat gacttcacgg tgatcaatca ggccgatctc
      361 ttgaacacgc taaacggcgt ggtggcgacg ctgacgctgt tcctcggcgc tatcgcgggg
      421 atctcgctgc tggtgggcgg tatcggcatc atgaacatca tgctggtctc cgtgaccgaa
      481 cgcacccgcg agatcggcat ccgcaaagcc gtcggcgccc agcagggcga catcctgcgc
      541 cagttcctga tcgaagcgat cctgatcagc gtgggcggcg ggttgctggg catcctgctc
      601 ggcttcatca tcgccaatct agtcacgctg accggcaccc ttacatccat cgtcacgccc
      661 ggcgccgcct tgctggcggt cggcttctcc ctggcggtgg gactgttctt cggcatttac
      721 ccggcccggc gcgcggcccg cctggacccg attgtggcgt tacgctacga ataacgcagt
      781 ccaaaggaga gattcgacaa tgcgcaatcc gtttgttatg ttagtggtgg gtctggtgat
      841 tgggttgatt atcgccgcgg gggcgttcat cggcggcatg aatatggggc aggcccaggc
      901 gcaggagcag caaaaccagt ttttcgccca gcgcggcttt aatccgaacg cggcgggcgg
      961 caccggggcc ggcggagccg gcggccagtt cggcggtgcg ggcggcaccg gcggcgccgg
     1021 gcgcacgggc ggcgcggcgg gccgcaacgc caccttcggc accatcaaga agatcgacgg
     1081 caacaccatt acactgaccg tgcccaacag cgaggacatc acggtgtcgc tgcccgccaa
     1141 cgccacgatc atcaagaccg tgaccggcac ggtgagcgac ctcaaagagg gccagcggct
     1201 gacggtcatg ggcacgcggt cgggcaataa cgtcgccgcg acagcgatcc aggtgacgga
     1261 cctgccggcg ggcgagaact tccccggctt tggcggcggg cgcaacggcg gggcgcgtgc
     1321 caccccgacg ccgggccagt aggccgcccc gtacgcgacg gccatgcgta cctgggtgct
     1381 gcgcatccgc gccgccgacc ggcccatctt tgatgcgctg gtcgacgggc gcaaaaccat
     1441 cgagacgcgc gccggggcta ccggcgcgcg ttcctatacc cgcgcgcagc cgggcgatac
     1501 gctgctgttc ctctgcggcc cggcccgcct gcgccgcatc gtgacgcggg tggcggtcta
     1561 cccgtcgccc gcggccctgg ccgcggacga agacgtgcgc cgggtgctgc cgtggataac
     1621 aagcgccgcc gacctggctg ccctctacga gtccttcccc ggctacccgg agcgcatcgc
     1681 ccgccacggc ctcgtggcga tggaactggc gccgccgctt gaggacacga cttgacggct
     1741 aaatatttag ctagtataat agctgctata gctgataagc ggtctgccta gcggtgtcaa
     1801 cccgaaaccg tgtattgaaa ggagagggcg caatgcctaa gatggtctcc gccaccgagg
     1861 ccaagaatcg gctcggggcg ctttttaact ggatacaaga taaccaggat acggtcatcg
     1921 tcgagagccg gggcgaaccc acggcagtga ttatgtccta cagcgaatac gagaaaatcc
     1981 aggcgttgaa ggagcaggca cgccgccagg agatatggga gcgcatggaa gccttgcggc
     2041 gcgaggtgcg ggcgaacaat cctgatataa cgactgagga gcaggccatg gagatagccg
     2101 atcagtttac tcgtgaagca attgcgagtt tagtgaagaa gggtaagatt aagttcaccc
     2161 agcccgagcc ataacaatgc gtgtgctttg cgatacgaat gtgttcataa gctacttact
     2221 tacgccggag agagcaggag tagttgccgc tatagtgcaa gcagcattcc aagaccgttt
     2281 cacacttatc cttcctgaag atgtcgtgca agagctgatc acagtaacca gggcaaaatc
     2341 gtatttatcc tctcgtattt cgctacaaac agtgacagcg tttgtacaag ttcttcaaga
     2401 cttggcggag tatctgccgg ctgttattga ggctattcct ccagtgtcga gagatccaaa
     2461 agacgactac ctgttggtgc tcgctttgat agggcgtgct gactacctgg taacaggtga
     2521 tgaggactta ttgtctttaa agcaggtaga gaccgtggcg attctccgtc cgagcgagtt
     2581 cgccgcgctg ctggcggaga cgtaagttcg catatgaacg acggggtatt cgcacgagaa
     2641 aggatccagt gattatgtcc gacagtgaat atgagaaaat
//
LOCUS       DASUYC010000502         3197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1410164, whole genome shotgun sequence.
ACCESSION   DASUYC010000502 DASUYC010000000
VERSION     DASUYC010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410164"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VF276_08250"
     CDS             <1..194
                     /locus_tag="VF276_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606890.1"
                     /translation="FLAATPTAAAAWPAQARAAGLPGFTTATAGPTLAPALTGATPPA
                     HPGPATTRGRVTVQPFTLP"
     gene            complement(222..1646)
                     /locus_tag="VF276_08255"
     CDS             complement(222..1646)
                     /locus_tag="VF276_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX6606891.1"
                     /translation="MKVKMILPALTEALSPYWRPIKYSLFPPLGLATLAAYLDAEDEV
                     TLQDEHVEPLDLTDSPDLVVIQVYITSAYRAYALADHYRAQGAYVALGGLHVTSLPDE
                     AVAHADTIFCGPGEDIWPAFLADFRAGRPGRVYRSTTRTLAGLPPIRRDLIKRGLYLC
                     PNSIVVSRGCPHVCDFCYKEAFYRGGKSFYTQAVDDALAEIARLPGRHVYFLDDHLFG
                     NARFAGALFEGMRGMGRVWQAAGTVKAVLQPGLLEQAVAAGLRSLFVGFETLNPANLA
                     AQRKYQNLDRDYNAAIRRLHDLGVMVNASFVFGMDDDDADVFDRTVAWAVEQGIETAT
                     FHILTPYPSTALYQRMAAEGRLLHSRWDLYDTRHVVYRPARLSAEELEAGYWRAYRAF
                     YRWGAIFRGAATHAGWRTRLRHVAYAGGWKKCEPFWDRVIRTHRVTRLLPLLETVLAA
                     FGAHSSPTRKLAIETPNGVAQLPS"
     gene            complement(1791..2744)
                     /locus_tag="VF276_08260"
     CDS             complement(1791..2744)
                     /locus_tag="VF276_08260"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEX6606892.1"
                     /translation="MSANLAPVLTQLASLFGVIVIGALLKVLGILPAELAPLVSRVVM
                     TLTLPALVFTAIRSAGAKPFSLDLLKMPLLAAIVIAASWVLAWLAARALRLNRPQTGA
                     FILASIFGSTAFLGYPIITALATVDKIGDTGQVAHVLYSELGTLVALVTFGLIVASIY
                     GEGAEISWRNLLAVPRAAPFIALMLGLLFYSDPLPPVITNLMNLMGQTTSFLMMLYLG
                     MSIVGTGVLAYWRPVLVSQGIKLLFAPLLGVLLVALLGMQGNSDLLKVAVIDSSTPSI
                     LLCLAYAAQYKLDLRLATTLVFSSFVLSLITMGLWLTLVIQ"
     gene            complement(2754..>3197)
                     /gene="tmk"
                     /locus_tag="VF276_08265"
     CDS             complement(2754..>3197)
                     /gene="tmk"
                     /locus_tag="VF276_08265"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX6606893.1"
                     /translation="PLGDEVMALLYAADRLDHLRQDIEPRLARGVDVVCDRYRLSSLA
                     YQSLTADLDWVAALNSKARRPDLTVFLDVPPEICRERMARRQGAVELYEQESKIRRVR
                     EHYLELIPAAEAAGERIVRVDGQGDVGTVAGLVWAACQAALPGLY"
BASE COUNT          552 a         1045 c         1138 g          462 t
ORIGIN      
        1 ggttcctggc ggccaccccc accgccgcgg cggcctggcc cgcccaggcg cgcgccgcgg
       61 gcctacccgg cttcaccacg gccaccgccg gccccacgct cgctcccgcg ctgaccggcg
      121 ccaccccgcc cgcccatccc ggcccggcga ccacccgcgg gcgggtcacg gtccagccgt
      181 tcacgctgcc ctgagcacga ccgggaacaa acagccgcca attaggacgg caattgcgcg
      241 accccattgg gcgtttcgat agccagtttc cgcgtgggac tcgaatgcgc cccaaacgcc
      301 gccagcactg tctccagcaa gggcagcagg cgtgtcacgc ggtgcgtccg gatgacgcga
      361 tcccagaacg gctcgcactt cttccagccg cccgcgtagg ccacgtggcg gaggcgcgtg
      421 cgccagccgg cgtgcgtggc ggcgccgcgg aagatcgccc cccaacggta gaaggcccgg
      481 taggcgcgcc agtagcccgc ttctaactcc tcggcgctca gccgggccgg gcggtagacc
      541 acgtggcgcg tgtcgtagag gtcccagcgc gaatgcagca accggccctc ggcggccatg
      601 cgctggtaga gcgccgtgct cgggtacggc gtgaggatgt ggaaggtcgc cgtctcgatg
      661 ccttgctcca ccgcccacgc caccgtgcgg tcgaacacgt ctgcatcgtc gtcgtccatg
      721 ccgaagacga agctggcgtt gaccatcacg cccaggtcgt gcaagcggcg gatcgcggcg
      781 ttgtagtccc ggtcgaggtt ctggtacttg cgttgggccg cgaggttggc cgggttcagc
      841 gtctcgaagc cgacgaagag gctgcgcaac ccggcggcga cggcttgctc caacaacccc
      901 ggctgcaaaa ccgccttgac cgtgcccgcc gcctgccaga cccggcccat cccgcgcatg
      961 ccctcgaata gcgccccggc gaagcgggcg ttgccgaaca ggtggtcgtc gaggaaatag
     1021 acatggcggc ccggcagccg ggcgatctcg gccagtgcat cgtccacggc ttgcgtgtag
     1081 aacgacttac ccccgcgata aaacgcctct ttgtagcaga aatcgcagac gtgcgggcag
     1141 ccccgcgaga cgacgatgga gttcgggcaa aggtagagcc cgcgcttgat caggtcgcgc
     1201 cggatgggcg gcagcccggc cagcgtgcgc gtggtcgaac ggtagacccg gcctggccgc
     1261 ccggcgcgga agtcggccag gaacgccggc cagatgtcct cgccggggcc gcagaagatg
     1321 gtgtcggcgt gcgccaccgc ctcgtcgggc agggacgtga cgtgcaggcc gccgagcgcg
     1381 acataggccc cctgcgcccg gtagtggtcg gccagcgcat aggcccggta cgccgaggtg
     1441 atgtagacct ggatcaccac caggtccggc gaatcggtca ggtccagcgg ctcgacatgc
     1501 tcatcttgca acgtgacctc gtcctctgcg tccaggtagg cggcgagggt ggcgagaccc
     1561 agcggcggaa agagggaata cttgatcggg cgccagtagg ggctgagcgc ctcggtcagc
     1621 gccggcaaga tcatcttgac tttcatcccg cgtccctcct ttatgataag aagcctgttt
     1681 agggcgatgg tcttatcata gggcgcggag atcggcgcga tctaggcttt gaggacccga
     1741 taggacgcac tcaggtgctt gattccagct ctctagcggg tgaggccggc ctactggatg
     1801 accagggtga gccagaggcc catggtgatg aggctgagga cgaagctgct gaagacgagg
     1861 gtggtggcga ggcgcaagtc gagcttatat tgggcggcgt aggccaggca gagcaggatg
     1921 ctgggcgtcg aggagtcgat gacggcgact ttgagcaggt cggagttgcc ctgcatgccg
     1981 agcagagcga ccagcagcac gccgagcagc ggggcgaaca gcagcttgat gccctgcgag
     2041 accagcacgg gccgccagta ggccagcacg ccggtgccga cgatgctcat gcccaggtag
     2101 agcatcatta agaacgaggt ggtctggccc atcaggttca tcaggttggt gatgaccggg
     2161 ggcagagggt cgctgtagaa cagcaggccc agcatgagcg cgataaacgg cgcggcacgg
     2221 ggcacggcca gcaaattccg ccagcttatt tcggcgccct ccccatagat cgacgccacg
     2281 atcagcccga acgtcaccag cgcgacaagc gtgccgagtt cgctgtagag cacgtgggcg
     2341 acctggccgg tgtcgccgat cttgtcgacg gtggcgaggg cggtgatgat cgggtagccg
     2401 aggaaggcgg tgctgccgaa gatcgaggcc aggatgaaag cgccggtttg cgggcgattg
     2461 aggcgcagcg cccgcgccgc cagccaggcc agcacccagg aggccgcaat gacgatggcg
     2521 gccagcagcg gcatcttgag caggtcgagg ctgaacggct tggcgcccgc gctgcggatg
     2581 gcggtgaaga ccagcgcggg cagcgtcagc gtcatgacga cgcgagagac cagcggggcc
     2641 agctcggcgg gcaaaatccc gagcaccttg agcagggcgc cgatgacgat gaccccgaac
     2701 aatgacgcaa gctgggttag gacgggcgcg aggttagcac tcatggttcg cgcctagtat
     2761 agcccaggca gggccgcttg gcaagccgcc cagaccagcc cggccaccgt gcccacgtcg
     2821 ccctgcccgt ccacgcgcac gatgcgctcg cccgcggctt cggcggcggg gatcagttcc
     2881 aggtagtgct cgcgcacgcg gcggattttg ctctcctgct cgtagagttc cactgcgccc
     2941 tggcgccggg ccatgcgttc gcggcagatc tccggcggca cgtcgaggaa gacggtcagg
     3001 tcggggcggc gggccttgct gttcagcgcg gccacccagt ccaggtcggc ggtgagcgac
     3061 tggtaggcca gcgaggagag gcggtagcgg tcgcagacca cgtccacgcc gcgcgccagg
     3121 cggggttcga tgtcctggcg caggtggtcg agccggtcgg cggcgtagag cagggccatg
     3181 acctcgtcgc ccagcgg
//
LOCUS       DASUYC010000503         3075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1218522, whole genome shotgun sequence.
ACCESSION   DASUYC010000503 DASUYC010000000
VERSION     DASUYC010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218522"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..1471)
                     /locus_tag="VF276_08270"
     CDS             complement(29..1471)
                     /locus_tag="VF276_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606894.1"
                     /translation="MADDTLVEGLPTLDPTANLMPFDTDDVPDGVRALEFLYLGQALT
                     AADFVDYVATYDFGTIPPDFVVLHHSAIPGTRFTGAADGGKQIWDAGEDGLGEAAIRA
                     HRLKGLEALRDFYKNTKKWDRGPHLYIDDRYIWLFTPMRELGIHAMWGNSFHDRQNRL
                     HYSIGIEVLGFYDHLKWPPEVARLVGHAVAVLKRRLGTFDLRYLYPNGNPGFFLDDDG
                     TAHCKHPEQLTFGGITSHRDYNKPGCPGAQISEAFYLDVLKAGQTALTTPAPAPVTTA
                     GASATGTAAGGGAVAFVPDDSAPVSDDPITADSLLLGRASGRRMQVINFIKARLPATS
                     EYKGDVETIVNFYWQYAPPAGIDPFLAAAQCVMETDTLQSPLAARPQRNPARLGLRDP
                     NAQSGGLAFATWELAVQAQLGHLLAYAVAAPANDAQRALLAQDPLLPSVPPADRGAGT
                     TLAGLNTRWSDDPTYGARLAAQATFIKTTG"
     gene            complement(1661..2299)
                     /locus_tag="VF276_08275"
     CDS             complement(1661..2299)
                     /locus_tag="VF276_08275"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6606895.1"
                     /translation="MKTDETRPPPRRGYHAPIRREQAELTRRRILDAARALFAERGYA
                     ATTVEAIAGRADVAVPTLYAALGSKRQILAALVAQLKDAYAVPARVRDLLADPDPRRQ
                     LEAAARITREFAVAAWDVIAVLDAARQADPELTELWDQVEGARLRDQQALAAQFAAAG
                     MLKPGLDAAQAGDIFWAMSGHDLYHRLVVERGWSADQYERWLLATLTALLLR"
     gene            2452..2877
                     /locus_tag="VF276_08280"
     CDS             2452..2877
                     /locus_tag="VF276_08280"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX6606896.1"
                     /translation="MSSQEHKAIARHFLQDAFPQGRFEQVLAPGYQDHNAPPGTLPGP
                     AGIAQITEPYRQAFPDLHFSIEDQVAEGDKVVTRFVFHGTHKGALMGIPPTGKPVHME
                     GISIYRIADGQMQEAWVQYDLLGLLQQLGAFPPAPAAVG"
BASE COUNT          497 a          986 c         1074 g          518 t
ORIGIN      
        1 cactgaccac tgaccactga ccactccgtc agccggtcgt cttgatgaac gtggcttgcg
       61 cggcgaggcg ggcgccgtag gtcgggtcgt cggaccagcg ggtgttcagg ccggccaggg
      121 tggtacccgc gccgcggtcg gcgggtggga cggaggggag cagcgggtcc tgggccagca
      181 gcgcgcgctg ggcgtcgttc gccggggcgg cgacggcgta ggccagcagg tggccgagct
      241 gggcctgcac ggccagttcc caggtggcga aggccaggcc gccgctttgc gcgttggggt
      301 cgcgcaggcc caggcgggcc gggttgcgct gcgggcgggc ggccaggggg ctttgtagcg
      361 tgtcggtctc catcacacac tgcgcggcgg ccaggaacgg gtcgattccc gcgggcggcg
      421 cgtactgcca gtagaagttg acgatggtct cgacatcgcc cttatactcc gacgtggcgg
      481 gcaggcgcgc cttgatgaag ttgatcacct gcatgcgccg gccactggcc cggcccagga
      541 gcagcgagtc ggcggtgatc gggtcgtcgg acacgggggc cgagtcgtcg ggcacgaagg
      601 cgaccgcgcc gccgccggcc gccgtgcccg tggcgctcgc gcccgccgtg gtgacggggg
      661 ccggcgccgg agtggtgagc gcggtctggc ccgccttcag cacgtcgaga taaaacgcct
      721 cgctgatctg cgcgccgggg cagccgggct tgttgtagtc gcggtgcgat gtgatgccgc
      781 cgaaggtaag ctgttccggg tgcttgcaat gcgccgtccc gtcgtcgtcg aggaagaagc
      841 ccggattgcc gttggggtag agatagcgca ggtcgaacgt gcccagccgc cgcttgagca
      901 cggccacggc gtggcccacc agccgcgcca cctcgggcgg ccacttcagg tggtcgtaga
      961 agcccagcac ctcgataccg atggaatagt gcagccggtt ctgccggtcg tggaacgagt
     1021 tgccccacat ggcgtggatg cccaattcgc gcatgggcgt gaacagccag atatagcggt
     1081 cgtcgatata gagatgcggc ccgcgatccc acttcttcgt attcttgtag aagtcgcgca
     1141 gcgcctccag gcccttgagc cggtgggcgc ggatggcggc ctcgcccagc ccgtcttcgc
     1201 ccgcatccca gatctgcttg ccgccgtcgg ctgcgccggt gaagcgggtg ccggggatgg
     1261 cgctgtgatg gagcacgaca aagtcgggcg ggatggtacc gaagtcatag gttgcgacgt
     1321 agtccacgaa atccgccgcc gtgagcgcct ggccgagata gaggaactcc agggcgcgca
     1381 cgccgtcggg cacgtcgtcg gtgtcgaacg gcatgaggtt ggccgtgggg tccagcgtgg
     1441 gtagaccctc aaccagagta tcgtccgcca tggcatccgt ctccttccac tacaacccgc
     1501 aggggccgcc ggctaccaac acgatccggc atccattttt acatagcaaa agttcactgc
     1561 aaagtaacgc agcacgtgaa aatgcgcggt cccggtggcg ttcttcgcgt ccttcgcgcc
     1621 ttcgcggtga aaaaatcccg tgatccgccg tacccctagc tcagcgcagc aagagggcgg
     1681 tgagggtggc gagcagccag cgctcgtact ggtctgcgga ccagccacgc tcgacgacca
     1741 ggcggtggta gaggtcgtgg ccgctcatgg cccagaagat gtcgcccgcc tgggccgcgt
     1801 ccagccccgg cttgagcatc ccggcggcgg caaactgggc ggccagggcc tgctggtcgc
     1861 gcaggcgcgc gccttccacc tggtcccaga gctcggtcag ttcggggtcg gcctgccggg
     1921 cggcgtcgag cacggcgatc acatcccagg cggccacggc gaactcgcgg gtgatgcggg
     1981 cggcggcctc cagttggcgg cgcgggtcgg ggtcggccag caggtcgcgc acgcgcgcgg
     2041 gcacggcata ggcgtccttc aactgcgcca ccagcgccgc caggatctgc cgcttggagc
     2101 cgagcgcggc gtagagcgtg ggcacggcca catcggcccg cccggcgatg gcctcgaccg
     2161 tggtggcggc atagccgcgc tcggcgaaca gggcgcgcgc cgcgtccagg atgcgccgcc
     2221 gggtcagttc ggcctgttcg cggcggatcg gggcgtggta gccgcgccgg ggcggcggac
     2281 gagtttcatc ggttttcatg caggtacttg catccttctt caatttaata tagaatggtt
     2341 atatccaatt gcatgtatct ataccgtatc gcccccatta tactcgatcc gggaacagga
     2401 tgcagcagtg gaccgttagt atcgaccctt atcggccaaa ggagatccgc catgtcgtcg
     2461 caagaacaca aagccatcgc ccgccacttt ttgcaggacg ccttcccgca gggccgcttc
     2521 gagcaggtcc tggcgcccgg ctaccaggac cacaacgcgc ctcccggcac cctgcccggc
     2581 cccgcgggca tcgcgcagat caccgaaccc tatcgccagg cgttccccga cctgcatttc
     2641 agcatcgaag accaggtggc cgagggggat aaggtggtca cgcgcttcgt cttccacggc
     2701 acccacaagg gtgccctgat gggcatcccg cccaccggca agccggtgca catggagggg
     2761 atcagcatct accgcatcgc ggacgggcag atgcaggaag cctgggtgca gtacgacctg
     2821 ctgggactgt tgcaacagtt gggcgcgttc ccgcccgccc ccgccgccgt cggctaaggg
     2881 cgcccgatct gtttcaccgc gaagacgcga aggacgcgaa gggtttgcga acggattgca
     2941 ccgcgaagca acgaaggcaa cgaagatttt tgagggtttt ggttcttgtg gtgagccggg
     3001 atgctcattc catgaaatcg ttaaaaccct tcgcgttctt cgtttcttcg cggtgacttt
     3061 ctataaaatc cttcg
//
LOCUS       DASUYC010000504         2449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_938368,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000504 DASUYC010000000
VERSION     DASUYC010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938368"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..1474)
                     /gene="accC"
                     /locus_tag="VF276_08285"
     CDS             complement(137..1474)
                     /gene="accC"
                     /locus_tag="VF276_08285"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008246424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX6606897.1"
                     /translation="MLDKVVIANRGEIALRILRACKEMGIRTVAVYSKADRDLLHVRL
                     ADEAVCIGPAASKDSYLNIPALISAAEVTDAQGIHPGYGFLAENADFAEAVEKSGFTF
                     IGPRPDSIRLMGNKVSAIEAMKKAGVPTVPGSGGPVTEENALVVAKRIGYPIIIKAAA
                     GGGGRGMRVVHTESALLNSLFVTRSEAKAAFGDDTVYMEKFLEHPRHVEIQVIGDGRG
                     KAVHLGDRDCSLQRRHQKVVEEAPAPHIPEKIRARVAESCVKACEQMKYRGAGTFEFL
                     YENEQFYFIEMNTRVQVEHPVTELVTGIDIIKEQLRVAAGLGLSVTQKDIRIQGHAIE
                     CRINAEDPKKFTPCPGKINYFHAPGGPGIRMDSHVYSGYTVPPNYDSLIGKLIAYGPT
                     RDVAFSRMRNALDEIVVDGIKTNIPLHRDEILRDANFAKGGVDIHYLEKKLGL"
     gene            complement(1488..1964)
                     /gene="accB"
                     /locus_tag="VF276_08290"
     CDS             complement(1488..1964)
                     /gene="accB"
                     /locus_tag="VF276_08290"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEX6606898.1"
                     /translation="MDIRKVKKLIELLEESGIDELEITEGEESVRIARHRTPVAATHV
                     VTAPAPVAAAAPAPQPAQASVEPTQGPAPAAVEPQTRGFVQRSPMVGTFYRAASPGAK
                     AFAEVGQVVKPGDVICIVEAMKMMNQIEAEQGGTIESILVENGQAVEFDQPLFTIV"
     gene            complement(2001..2441)
                     /gene="aroQ"
                     /locus_tag="VF276_08295"
     CDS             complement(2001..2441)
                     /gene="aroQ"
                     /locus_tag="VF276_08295"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEX6606899.1"
                     /translation="MLVLNGPNLNLLGSREPGIYGADTLDSINHRLAARAREAGIALD
                     CFQSNHEGALVDRVHAARLDGTRFVLINPGAFTHTSVALRDALLGVGLPFVEIHLSNV
                     HAREEFRRHSYFSDVAVGVICGLGAQGYELALEFALQRLGTAAT"
BASE COUNT          390 a          732 c          894 g          433 t
ORIGIN      
        1 cgggagcctg tgccttggtg gtgtcaatct tcagttgcag ccagctcatc agcaaatttc
       61 caacgcaaaa aaatcaaaag cggagtgtag ttgcccgcac tccgcgttta cagacaggca
      121 ggaatgagga ccggcctcac aggcccagtt tcttttccag gtaatggatg tccaccccgc
      181 ccttggcgaa attggcgtcg cgcaggatct cgtcgcggtg cagggggatg ttggtcttga
      241 tgccgtccac cacgatctcg tccagggcat tgcgcatgcg cgagaaggcg acgtcgcgcg
      301 tcggcccgta ggcgatgagc ttgccgatca gggagtcgta gttgggcggc accgtgtagc
      361 ccgagtacac atgcgagtcc atgcggatgc cggggccgcc gggggcgtgg aagtagttga
      421 tcttgcccgg gcagggcgtg aacttcttcg ggtcctcggc gttgatgcgg cactcgatgg
      481 catggccctg gatgcggatg tccttctggg tcaccgacag gccgaggccg gccgccacgc
      541 gcagctgctc cttgatgatg tcgatgccgg tcaccagctc ggtcaccgga tgctccacct
      601 ggacgcgggt gttcatctcg atgaagtaga actgctcgtt ctcgtagagg aactcgaagg
      661 taccggcgcc gcgatacttc atctgctcgc aggccttgac gcaggactcg gcgacgcggg
      721 cgcggatctt ctcggggatg tgcggggcgg gcgcctcttc caccaccttc tggtggcggc
      781 gctgcagcga gcagtcgcgg tcgcccaggt gcacggcctt gccgcggccg tcgccgatga
      841 cctggatctc cacatggcgg ggatgttcca ggaacttctc catgtagacg gtatcgtcgc
      901 cgaaggcggc cttggcctcg gagcgcgtca cgaacagcga gttcagcagc gccgactcgg
      961 tgtgcaccac gcgcatgcca cggccgccac caccggcggc ggccttgatg atgatgggat
     1021 agccgatgcg cttggcgacg acgagggcgt tttcctcggt caccgggccg ccggagccgg
     1081 gcacggtggg cacgccggcc ttcttcatgg cctcgatggc cgagaccttg ttgcccatca
     1141 ggcggatgct gtccgggcgc gggccgatga aggtgaagcc ggacttttcc acggcttccg
     1201 caaagtcggc gttctcggcg agaaagccgt agccggggtg gatgccctgg gcgtcggtga
     1261 cctcggccgc cgagatcagc gccgggatat tgagatagct gtccttggac gcggccgggc
     1321 cgatgcagac cgcctcgtcc gccaggcgga catggagcag gtcgcggtcg gccttggagt
     1381 agacggcgac cgtgcggatg cccatttcct tgcaggcgcg caggatgcgc agcgcgattt
     1441 cgcctcggtt ggcgatgacc accttatcga gcatgacggg gccccgctca gacgatggtg
     1501 aagagcggct ggtcgaattc cacggcctgg ccgttctcga ccaggatgct ttcgatggtg
     1561 ccgccctgct cggcctcgat ctggttcatc atcttcatgg cctcgacgat gcagatgaca
     1621 tcgcccggct tcacgacctg gccaacctcg gcgaaggcct tggcgcccgg cgaggcggcg
     1681 cggtagaagg tgccgaccat gggcgagcgc tgcacgaagc cacgggtttg cggttcgacg
     1741 gcggcggggg cggggccctg ggtgggctcg acgctggcct gggccggctg gggggcgggc
     1801 gcggcggcgg cgacgggagc cggggcggtc acgacatggg tggcggcaac gggcgtgcgg
     1861 tgacgggcga tgcgcaccga ctcctcgccc tcggtgatct cgagttcgtc gatgccggac
     1921 tcctccagca gctcgatgag tttcttgacc ttgcgaatat ccatgggtac ttccggttaa
     1981 aagtgaaaaa cggtgaagga tcaggtcgcg gccgtgccga ggcgctgcag ggcgaattcc
     2041 agggcgagtt cgtagccctg ggccccgagg ccgcagatga cgccgaccgc gacgtcggag
     2101 aaataggagt gccggcggaa ttcctcgcgg gcatgcacgt tcgagaggtg gatctcgaca
     2161 aaggggaggc cgacgccgag cagggcgtcg cgcagggcca ccgaggtgtg cgtgaaggcg
     2221 ccgggattga tcaggacgaa gcgggtgccg tcgaggcggg cggcgtggac gcggtccacc
     2281 agggcgccct catggttgct ctggaagcag tcgagggcga tgccggcctc gcgcgcgcgg
     2341 gcggccaggc ggtggttgat gctgtccagg gtgtcggcgc cgtagatgcc gggctcacgg
     2401 ctgcccagca ggttcaggtt ggggccgttc aggacaagaa tgccggtca
//
LOCUS       DASUYC010000505         3402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_948166,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000505 DASUYC010000000
VERSION     DASUYC010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948166"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..153)
                     /locus_tag="VF276_08300"
     tRNA            complement(79..153)
                     /locus_tag="VF276_08300"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(117..119),aa:His,seq:gtg)
     gene            537..1262
                     /locus_tag="VF276_08305"
     CDS             537..1262
                     /locus_tag="VF276_08305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsnF/AvaK domain-containing protein"
                     /protein_id="HEX6606900.1"
                     /translation="MQTNECRPGMAVECLGHRLGTLSGVEGTGAAQMLAIRPDKGHTN
                     EPLLQIPGRYIAQVLDNTVLLNITCEEATEIGRGATPAAVPASPDVETTPAAPIVTDI
                     APGEPLTVPLVEETLVPVTQWHEAGALEVHKTVQTVTQELDVPVRYEEATVERVPLNR
                     VLADDEQPAQRQEGDTLIVPVVREELVVVKQRILVEELRITKQTRTVTRHFAEPVRRE
                     EVTFSHEGLEAQPTESVPAPPEQ"
     gene            1385..2479
                     /locus_tag="VF276_08310"
     CDS             1385..2479
                     /locus_tag="VF276_08310"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HEX6606901.1"
                     /translation="MTNRTTKRETVVGVFNDRDTAQAAIEQLKNAGFRPEDIGILMQN
                     KEGAQDLAADTGTKAGEGAGKGAVTGGILGGLGGLLVGIGALAIPGIGPVVAAGPLAA
                     GLGAVLGTTATGAVIGAGVGAIAGALIGMGVPEDEARVYEERFNQGRILVTVKAGANR
                     YNEAERILRNAGAEQIDFRNAGAGSYTQGQTGYAQNQAGYTQGQTGYTQAETGDRMRV
                     PVVEETLNVEKGQREAGQVEIRKNVREEQVNVPVELRHEEVTVTRHAADRPLQPGEQV
                     LEDDQVIRVPVREETADVQKQARVREEIEINKQQVAEQRNVSDTVRREEVDVDTQGNV
                     RAREGQGGQYNRTTGTNYNEDTDTTYNQNQ"
     gene            2665..>3402
                     /locus_tag="VF276_08315"
     CDS             2665..>3402
                     /locus_tag="VF276_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6606902.1"
                     /translation="MTLAETGPPLVQATGLVRTFGPVRAVDGIDLEVQPGEVFALLGP
                     NGAGKSTTIKMLITLLPPTAGHATVGGYDIVREAGAVRRLLGYVPQALSADGALTGYE
                     NLLVFARLYDVPRPEQKDRIWAALEFMGLAGAADRLVRNYSGGMVRRLEIAQALIHHP
                     RILFLDEPTIGLDPVARRAVWEHLTRLREELGSTLFLTTHYMEEADDLATRIAIMSQG
                     RISALGTAEELKAQVPGGAATLEDVFVY"
BASE COUNT          636 a         1090 c         1172 g          504 t
ORIGIN      
        1 ccggcgtcct accagttgaa cgacttccca atgcgcgcca gatacggcgc cggacgggtc
       61 ggatgccggc ctggcgtggg gtgagcgagg ggactcgaac ccctgaccac ccgatccaca
      121 atcgggcgct ctgcctgctg agctacgcgc accatgggcg atgaaacgcg ggcggcgggc
      181 gggcccgcgc gcgctgccgc ataggtcgac ggccgggcaa tcccgcgctg gagggcggca
      241 gaagtggttt gcgataacgc tcacccttcg gcccggccat cccgatctta cggggaatcg
      301 cgcggcgagg tcaatcgccc gcgcggcgga atcggctcgg tctgcgggcc gaatttgggc
      361 gtggcggatc tacacccccg gtcccgtgcg ggcgatgtgg cacgcggctt gcttgatata
      421 atagggctta ggaaagtggg atgcaatatg gtcccataat ctacatgagt gggatcgccg
      481 gcccatcggg ccggttgatt tcccgccagg tgctacggag tagataaggg ataatcatgc
      541 agaccaatga atgccgcccc ggcatggcgg ttgagtgcct gggccaccgg ctcggcaccc
      601 tcagcggcgt ggaaggcacg ggcgccgcgc agatgctcgc catccggccc gacaaagggc
      661 acaccaacga accgctcctc cagataccgg gccgctatat cgcccaggtg ctggacaaca
      721 cggtgctgct gaatatcacc tgcgaggaag ccaccgagat tggccggggc gcgaccccgg
      781 cggcggtgcc ggcctccccc gatgtggaga ccaccccggc tgcgcccatc gtgaccgata
      841 ttgctcccgg cgaacccttg acggtgccgc tcgtcgaaga gaccctggtc ccggtgacac
      901 aatggcacga agcgggcgcg ctggaggtcc acaaaaccgt gcagaccgtc acccaggaac
      961 tcgacgtgcc ggtacgctac gaagaggcca cggtcgagcg cgtgccgctc aaccgtgtgc
     1021 tggccgacga cgaacagccc gcgcagcgcc aggagggcga caccctgatt gtccccgtgg
     1081 tgcgcgaaga gctggtcgtt gtcaaacaac gcatcctggt cgaagagctg cgcatcacca
     1141 agcagacacg cacggtgact cgccatttcg cggagcctgt ccggcgcgaa gaagtcacct
     1201 tttcccacga agggctggaa gcccaaccaa ccgaatcggt accggcgccc ccggagcaat
     1261 agttccctgg cgccttgacg cgggatctac cggggatcac cgcgggctgg attgactggc
     1321 cgtgccgtta gagaccgacc gtagtgccac cagatggcta tcaaacagct aaaggagata
     1381 tcacatgacc aatcgaacta cgaagcgcga aaccgtcgtc ggcgtgttta acgaccggga
     1441 cacggcgcaa gccgcaattg agcagttgaa gaacgccggg ttccgccccg aagatatcgg
     1501 tatcctgatg cagaacaagg aaggcgcgca ggatctggcc gcggacaccg gcacgaaggc
     1561 cggcgagggc gccgggaagg gcgccgtgac cggcggtatc ctcggtggcc tcggcggctt
     1621 gctcgtgggc atcggcgcgc tggcgattcc gggcatcggc ccggtggtgg ccgcggggcc
     1681 gctggccgcg ggcctaggcg ccgtgctcgg caccaccgcc accggcgcgg tcatcggcgc
     1741 gggcgtgggc gccattgccg gtgccctgat cggtatgggc gtgcccgagg acgaggcccg
     1801 tgtgtacgag gagcgcttca accagggccg catcctggtc accgtgaagg ccggcgcgaa
     1861 ccgctacaat gaggccgagc gcatcctgcg caacgccggg gccgaacaga tcgacttccg
     1921 caacgccggc gcgggcagct acacgcaggg ccagaccggc tatgcgcaaa accaggccgg
     1981 ctatacccag ggccagaccg gttacaccca ggccgaaacc ggggatcgca tgcgcgttcc
     2041 cgtggtcgag gagaccttga acgtcgagaa gggccagcgc gaggcgggcc aggtggagat
     2101 ccgcaagaac gtgcgcgagg agcaggttaa tgtgcccgtc gaattgcgcc atgaggaagt
     2161 gaccgtgacc cgccacgcgg cggatcgccc gctgcagccc ggcgagcagg tgctggagga
     2221 cgaccaggtg atccgcgtgc ccgtgcgcga ggagacggcg gatgtgcaga agcaggcccg
     2281 cgtgcgcgaa gagatcgaga tcaacaagca gcaggtcgcc gagcagcgca acgtgagcga
     2341 tacggtgcgc cgcgaggaag tcgatgtcga tacacaaggc aacgtgcggg cgcgcgaggg
     2401 ccagggcggc cagtacaacc gcacgaccgg caccaactac aacgaggaca ccgacaccac
     2461 gtacaatcag aaccagtagt ctgccggccg tttcgacggc acgcgacttt gcgcggcccg
     2521 gatgggaaac cgtccgggct tcgctgtgcg tgtacctgga tgggtttgcc gggtttgcga
     2581 gagacctgat atcagcacga ttaatcccta ttcgggcgat cgcggttgtc cttcgcggct
     2641 ttaggagtcg gagatgaggg caaaatgacc ttagcggaga cagggccgcc cctggtgcag
     2701 gccacgggtc tggtgcgcac ctttggcccg gtgcgcgcgg tcgatgggat cgacctggaa
     2761 gtgcagcccg gcgaagtctt tgccctgctg ggaccgaacg gggcgggcaa atcgaccacg
     2821 atcaagatgc tgatcacgct gttgccgccc accgcgggcc acgccaccgt gggcggctac
     2881 gatattgtgc gcgaagccgg ggccgtgcgc cgcttgctcg gctatgtgcc gcaggcgctc
     2941 tccgccgacg gtgcgctgac cggctacgag aacctgctgg tcttcgcgcg tctttacgat
     3001 gtccctcgcc cggagcagaa ggatcggatc tgggcggcgc tagagttcat ggggctggcc
     3061 ggcgccgccg accggctggt gcgcaattac tccggcggca tggtgcgccg cctcgaaatc
     3121 gcccaggcgc tgatccacca cccgcgtatc ctgttcctgg atgagccgac tatcggcctc
     3181 gacccagtgg cccggcgcgc ggtctgggaa cacctcaccc ggctgcgcga agagctcggc
     3241 tccacgctct ttttgaccac gcactatatg gaagaggcgg acgacctggc gacccgcatc
     3301 gctatcatga gccaggggcg gatctccgcg ctgggcaccg ccgaggaact gaaagcccag
     3361 gtgccggggg gcgcggccac gctcgaagac gtttttgtct ac
//
LOCUS       DASUYC010000506         3853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1912322, whole genome shotgun sequence.
ACCESSION   DASUYC010000506 DASUYC010000000
VERSION     DASUYC010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1912322"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1820)
                     /locus_tag="VF276_08320"
     CDS             complement(<1..1820)
                     /locus_tag="VF276_08320"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX6606903.1"
                     /translation="MPAPHRLIGCALLLIALAGGSLARGSADPAQPAPSDGAGTLSAV
                     AVSGSEIDLTWSPAVSGTQPILLRRDKALMTTLTAGTTRYTDRGVQPGTLYRYTLEPA
                     GGAAGRASLPTVLIRTPALPDLPDRTPPTPPEGLSAVGGAGWVLLDWAAALDDSEVTA
                     YRVRRNGQVLATVDGGTLRFVDSALPANAVYHYTVTAFDVAGHESAPTESVQAQVGPG
                     PAGPLPMAQPSLPAPDAPLTYAGALRRYPYLTDVVGPYASVNWATDRSAPTGSIAWGR
                     VGSESCTAHTAPATRTSISVNGVATYQWTVPLTLTLNTQYCYRVYLGSTDLLGSDPAP
                     QFWTQIPAGASQPFSFAVFGDWGAVDAGGANPDQANLLRQLASSGARFALTVGDNAYP
                     SGSQNNYGDLRQTGTNLSGVFGPLFWTKVGARLPLFPSIGNHGFSRADTYHPHLLNWP
                     QSQAVATSGGRYTMETYCCLNGTRSAVYPSTWYAFDAGVARFYMLEATWDEANEGTAS
                     VYANDYAYHWTAGSAEYQWLQQDLAGHPAGLKFAFFHYPLYSDNSTEPADTFLRGPQS
                     LEGLLNRYGVAIAFSGHAHIYQRNTAPNAGSLITYLTGGGG"
     gene            2702..>3853
                     /locus_tag="VF276_08325"
     CDS             2702..>3853
                     /locus_tag="VF276_08325"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEX6606904.1"
                     /translation="MIDRMARMRARLPHIAYGGDYNPEQWTPAFGYEDERIWHDDMRL
                     MRLAHVNVATVGVFSWVALQPDETTFTFGWLDRIMDLLAENGIFACLGTGTAAQPAWL
                     SAAYPDVLPVDAQGLRRAHGQRQNYCPTSPDFRRLAAGLARRLAERFREHPALLLWHV
                     SNEYGPACYCDRCAARFRAWLQARYGTLDEVNRRWQTAFWSHSYTEWAQIHPPGPRGE
                     ASIQGLTLDYRRFMSDMNLECYRGEADVLHAVTPDVPVTTNFMRFYKPLDYFAWAPYL
                     DVIAWDSYPQREDHPSSTALMHDLMRGLKGGQSWMLMEQTPSQVQWRPYNPLKRPGVL
                     RLWSYQAVAHGADAVMYFQWRQSRGSAEKYHGAIVSHAGHEHTRVFGEVA"
BASE COUNT          654 a         1228 c         1343 g          628 t
ORIGIN      
        1 cctccgccgc ccgtcaggta ggtgatgagg ctgccggcgt tgggcgcggt gttgcgctgg
       61 tagatatggg cgtggccgct gaacgcgatg gcgaccccat agcggttgag caggccttcc
      121 aggctctgcg gcccgcgcag aaacgtatcc gccggttcag tgctgttatc tgagtagagc
      181 ggataatgga agaaggcgaa cttgagcccc gccggatggc cggccaggtc ttgttgcagc
      241 cattggtact cggcactgcc agcggtccag tggtaggcgt aatcgttggc atacacactc
      301 gccgtgcctt cgttggcttc atcccaggtc gcttcgagca tgtagaaccg ggccacgccc
      361 gcgtcgaaag cataccaggt gctcgggtag accgcggacc gggtgccgtt gaggcaacag
      421 taggtctcca tggtgtagcg cccgccggag gtcgccacgg cctggctttg cggccagttg
      481 agcaggtgcg gatgataggt atcggcccgg ctgaacccgt ggttgccgat actggggaac
      541 aacggcaggc gcgcgcccac cttggtccaa aagagcgggc cgaacacccc gctgaggttg
      601 gtgccggttt gccgcagatc gccgtagttg ttctggctgc ccgacgggta ggcgttatcg
      661 ccgacggtga gcgcgaagcg ggccccactg ctcgcgagtt ggcgcaacag gttggcctga
      721 tcgggattgg cgccgccggc gtccactgcg ccccaatcgc cgaacaccgc gaagctgaac
      781 ggctgggacg ccccggcggg gatctgggtc cagaactggg gcgccggatc gctgccgagc
      841 aggtcggtgc tgcccaggta gacgcgatag cagtactggg tattcagcgt gagcgtgagc
      901 ggcacggtcc actgataggt ggcgaccccg ttgacgctga tcgaggtgcg agtcgcgggg
      961 gccgtgtggg cggtgcagga ctcactcccg acccggcccc aggcaatgct gccggtgggg
     1021 gcggagcgat cggtcgccca gttgacgctc gcatagggcc cgaccacgtc tgtcaggtag
     1081 ggataccgcc gcaacgcacc ggcatacgtc agcggggcgt cgggggccgg cagcgacggc
     1141 tgcgccatag ggagcggacc ggccggaccg gggccaacct gcgcctgcac ggactccgtg
     1201 ggggccgact cgtgtccggc cacgtcgaag gcggtcacgg tgtagtggta gacggcattg
     1261 gcgggcagag cggagtccac aaagcggagg gtgccgccgt cgaccgtcgc cagaacctgc
     1321 ccattgcgcc gcacccggta ggcagtgacc tcggagtcat caagggccgc tgcccaatcg
     1381 agcaacaccc agccggcgcc gccgaccgca ctcaagcctt cgggcggggt gggcggcgtg
     1441 cggtcgggca gatcgggcag ggcaggagtg cgaatcagca cagtgggcag ggatgcccgt
     1501 cccgccgcgc cgccggccgg ttccagtgtg tagcgataga gcgtccccgg ctgcacgccg
     1561 cgatcagtgt agcgcgtcgt gccggccgtc agggtggtca tgagtgcctt gtcgcggcgc
     1621 agcaggatgg gctgggtacc cgacacagcc ggggaccagg tcaagtcgat ctcgctgccg
     1681 ctgacggcga ccgctgagag tgtgcccgcc ccgtcggacg gggcgggttg cgccggatcg
     1741 gcggatccac gggccaggga tccgcctgcc agggcgatga gcagcagggc acagccgatc
     1801 aggcggtgtg gcgcgggcat gactcctacc tcctgggcgc ggatcccacg ggacggggat
     1861 tcgcgggtgc ttaggccggg tgagccggcc gttaatggcg gaaggtcaat aaacctccgc
     1921 cccgccccgc gcgagcgggt ggtacgactg ggggtggacg gtcgcccggt gagccgccgg
     1981 ctgactgccg gctgggaaac gcagggccgc cccgccggcg gtggtccccc cgccgatgct
     2041 gcccgcgccc agggcataac cggcaggcgt ccgggccccg cgtcaggaag gcttcgcggc
     2101 gtagggcacg aactcgataa tagaagatcg gcagcgcttt ctacggtgcc aagcaaccga
     2161 ggaggatcag gggtttggga tgcaggcact gcgccgggtg ccatcaaacc tgcgcaggga
     2221 cgtaccgcat cttagcagaa actaaaacgg tctcataaat agaccctcga tgccaactta
     2281 acgcagcaag ttgcgatcca tcaaagttat gagaccaatt ctagtatagt acacccggca
     2341 cgttcaagca atcgcagaat cttacaatct gtttagaaac agcaacaagc aatttctgtc
     2401 taaacggcgc tgctgcggcg ctgtaccgtt ccgctcggac catcgagcct acaacgataa
     2461 ccgggatctc gttgctaagc gcggagttat cttcatccca gcccggtgta gctgaactgg
     2521 ttgccgcgcc aaaggttctt ctctgtacga agctcggcgg cgccgcactg cgggcatcac
     2581 cgccgccggt ggcgtaaagc ttgctggcgg gcatcccgcc gggcccgccg tgccatcttg
     2641 tgatcgcccg tggcagggcg gcgtaagagg ggacttccct ggcttgaatg gagaaaaacc
     2701 gatgatcgat cgtatggcgc ggatgcgcgc tcgcttgccc cacatcgcct atggcggcga
     2761 ctataatccc gagcagtgga ccccggcctt cggctacgag gacgagcgca tctggcacga
     2821 cgatatgcgc ttgatgcgcc tggcccacgt caacgtggcc acggtcggcg tctttagctg
     2881 ggtcgcgctg caacccgacg aaaccacctt caccttcggc tggctggacc ggatcatgga
     2941 tctgctggcg gaaaacggca tcttcgcctg cctcggcacc ggaaccgccg cgcaacccgc
     3001 ctggttgtcg gcggcctacc ccgatgtgct gcctgtggac gcacaggggc tgcgccgcgc
     3061 gcatgggcag cgccagaatt actgtccgac cagccccgac ttccgccggc tggcggccgg
     3121 gctggcgcgg cgcctggccg agcgcttccg ggagcatccg gcgctgctgt tgtggcatgt
     3181 gagcaacgaa tatgggccgg cctgctactg tgatcgctgc gccgcccggt tccgcgcctg
     3241 gctgcaggcc cgctacggca ccctggacga ggtcaaccgg cgctggcaga ccgccttctg
     3301 gagccatagc tacaccgagt gggcgcaaat ccacccgccc ggcccccgtg gcgaggcgag
     3361 tatccagggc ttgaccctgg actaccggcg ctttatgtcg gacatgaacc tggaatgcta
     3421 ccgcggcgaa gccgatgtcc tgcacgcggt gacgcccgat gtcccggtga ccaccaactt
     3481 catgcggttc tacaaacccc tggactactt tgcctgggcg ccctacctcg acgtgatcgc
     3541 ctgggactcc tatccgcagc gggaggacca tcccagttcc acggcgctga tgcatgacct
     3601 catgcggggg ctcaagggcg ggcagtcctg gatgctgatg gagcagacgc cgagccaggt
     3661 gcagtggcgc ccatataacc cgctcaagcg gcccggcgtg ctgcgcctgt ggagttacca
     3721 ggccgtggcc cacggggccg acgcggtgat gtatttccag tggcgccagt cgcgcgggtc
     3781 ggcagagaag taccacggcg ccatcgtgag ccacgccggg catgagcaca cgcgcgtgtt
     3841 cggcgaggtg gcc
//
LOCUS       DASUYC010000507         3417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_965954,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000507 DASUYC010000000
VERSION     DASUYC010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965954"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1547)
                     /locus_tag="VF276_08330"
     CDS             complement(<1..1547)
                     /locus_tag="VF276_08330"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HEX6606905.1"
                     /translation="MIAPPPAPRPGPPPTLAALRGALQHWFGFQDFRPGQEAVITAVM
                     GGQDTLALMPTGAGKSLCYQLPAMLLPGLTLVISPLIALMKDQYDSLPKPVYERSTFI
                     NSSLDIGEVERRMADVLAGKIKLIYAAPERLRQQPFVHALARAGLALLVIDEAHCVSL
                     WGHDFRPDYLFIGKVLPALGNPRLLGMTATATERVQQEIGAQLGRPLHIVQTSPFRPN
                     LFYQVMHLKQQKEKTEALVRFCRDQKGSGIVYVRSRQQAEELAYTLRNRGVKAEHYHA
                     RLDPAERSAVQERWMLDRTRVIVATIAFGMGIDKQNVRFIVHFSPPDSVESYAQESGR
                     AGRDGRPSICLMLATPGDRSNLTRWLREAAFNIDKLRTIYGAARQQVRAVGRPTLVNF
                     GQMLQDTAANTGQPLDDTELRVGIGLMERVDLLVRHADAPANVGVQVQGALLAAPDPQ
                     FDRFLAACDLLPGRRAELRLSEIAGYLEMSPLALEAALLRWRDEDRIVLRSGGREPVI
                     ERLPPPA"
     gene            complement(1685..2356)
                     /locus_tag="VF276_08335"
     CDS             complement(1685..2356)
                     /locus_tag="VF276_08335"
                     /inference="COORDINATES: protein motif:HMM:NF025779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm5 family immunity protein"
                     /protein_id="HEX6606906.1"
                     /translation="MILSPELLVLLEQASAALRQELDYSLKPIQRIRIYKVFGSLAAE
                     QAQRLRALLAIYAAQQVLPRWHESRPTDFRPEQLLNVALRVLHGTVPDKVALAKALAV
                     AGVLREGNRSRIEVFFRGEPFTYRDYFVIDTALEAIVEVCGDSLFEKSAISEGTKDLD
                     LDWWHKDTASRAAIAYAGAVSDPTGDPNKRREFWEWWLWEAIPLAWSKSISGHALPPL
                     YPLPV"
     gene            complement(2448..3170)
                     /locus_tag="VF276_08340"
     CDS             complement(2448..3170)
                     /locus_tag="VF276_08340"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX6606907.1"
                     /translation="MSRPLTIGIVGGPFPVAMFRYDQQIGTRTADTGDDGAAPQMVGG
                     RAPCQQYLRYLQWQHLGAWEELAYEIEQECEAVAAAGADFAILAPPALYEVLPLVHSP
                     LPIYHILDAVADYAQAHNLPRLALAGPRLTGQARFYGNGLRERGVPVLLPTDAEQRTL
                     DRLVYTQIIRGRVTPALSRDFAEVARSLARRGAAGILLTCAELELLARDQALPIPTID
                     GTRVHLDLAWEIATGKRSFVAS"
     gene            complement(3219..>3417)
                     /locus_tag="VF276_08345"
     CDS             complement(3219..>3417)
                     /locus_tag="VF276_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HEX6606908.1"
                     /translation="TTAELIRARVPATRVVMLTLYDETPLSNRARAAGASAFVAKHER
                     VDVLLAAIRQAASEAAAAPLD"
BASE COUNT          580 a         1046 c         1181 g          610 t
ORIGIN      
        1 tcggcggggg gcggcaggcg ctcgatcacc ggctcgcgcc cgccgctgcg cagcacaatg
       61 cggtcctcgt cgcgccaacg cagcagcgcc gcctccaggg ccagcgggct catctccagg
      121 tacccggcga tctcgctcag gcgcagttcg gcgcgccggc ccggcagcag gtcgcaggcg
      181 gccaggaagc ggtcgaactg cgggtcgggc gcggccagga gcgccccctg gacctgcacg
      241 cccacgttcg ccggggcgtc ggcgtggcgc accagcaggt ccacccgttc catcaggccg
      301 atgccgacgc ggagttcggt gtcgtccagc ggctggccgg tgttcgccgc cgtatcctgc
      361 aacatctggc cgaagttgac cagggtgggg cggcccacgg cgcgcacctg ctgccgcgcg
      421 gccccgtaga tggtgcgcag cttgtcgatg ttgaacgccg cctcgcgcag ccagcgggtg
      481 aggtttgagc ggtcgccggg cgtggcgagc atcaggcaga tcgaggggcg cccgtcgcgg
      541 cccgcgcgcc cgctctcctg ggcgtagctc tccaccgagt cgggcgggct gaagtggacg
      601 ataaagcgca cgttctgctt gtcgatgccc atgccgaagg cgatggtcgc cacgatgacc
      661 cgcgtgcggt cgagcatcca gcgttcctgc acggcgctgc gttcggcggg gtcgagccgg
      721 gcgtggtagt gttcggcctt gacgccgcgg ttacgcaacg tataggctaa ttcctcggcc
      781 tgctgccggg agcgcacgta gacgatgccc gatcccttct gatcgcggca aaagcgcacc
      841 agcgcctcgg ttttttcctt ctgttgcttg agatgcatca cctggtagaa caggttgggg
      901 cggaaggggc tggtctggac gatatgcagc gggcggccca gttgcgcacc gatctcctgc
      961 tgcacgcgct cggtggcggt ggccgtcatg cccagcaggc gcgggttgcc cagcgcgggc
     1021 aagaccttgc cgatgaacag gtaatcgggg cggaaatcgt gaccccagag gctgacgcag
     1081 tgcgcctcgt cgatgaccag cagggccagc ccggcgcggg ccagcgcgtg gacgaagggc
     1141 tgctggcgca accgctcggg cgcggcgtag atcagcttga tcttgcccgc cagcacgtcg
     1201 gccatgcgcc gctcgacctc gccgatgtcg aggctgctgt tgatgaaggt gctgcgctcg
     1261 tagaccggct tgggcaggct gtcgtactgg tccttcatca gcgcgatcag cggggagatg
     1321 accagggtga ggcccggcag cagcatggcc ggcagttggt agcagagcga cttgccggcg
     1381 ccggtgggca tgagcgccag cgtatcctgc ccgcccatga cggcggtaat gacggcctcc
     1441 tggccgggac ggaagtcctg gaacccgaac cagtgctgca aggcgccgcg caaggcggcc
     1501 agggtgggcg gcgggccggg cctgggcgcg gggggcgggg cgatcatgcc tctattatac
     1561 cgtattagcg gtcagccgtc agccgtcagg agacgagtgg tcagtggtca gtggtcagtg
     1621 atcagtatgg cgaatacaga ggactaaggc cgaggatggt gaatagtccg tgatcaaagc
     1681 gggatcacac aggcaaggga taaagcggag gaagggcatg gcccgatatg gatttactcc
     1741 aagctaatgg gatggcctcc catagccacc actcccagaa ctctctcctc ttattagggt
     1801 ccccagttgg gtcacttact gctccggcgt atgctatggc tgctcttgat gcagtatctt
     1861 tgtgccacca gtccagatcc agatctttag tgccttcact aatagcgctc ttctcgaaaa
     1921 gagaatcacc gcatacctca acaatagctt cgagtgccgt gtcgataaca aaataatccc
     1981 gataggtaaa aggctctcca cgaaagaata cttcaatcct gctgcgatta ccttcgcgta
     2041 gcacacctgc aactgcaagt gctttggcaa gggccacttt gtctggtact gtcccatgta
     2101 acacgcggag cgcaacattc aggagttgtt caggacgaaa atcagtaggt ctagactcgt
     2161 gccatcttgg gaggacttgc tgagcagcat atattgctag gagagcacgt agcctctgtg
     2221 cttgttcagc ggctagcgat ccaaatactt tgtaaatcct aatgcgctga attggtttga
     2281 gggaatagtc cagttcctgc cggagagccg cagatgcctg ctctaacaag accagtaatt
     2341 caggcgacag gatcatggat cttgcttctt atattgtggc ttgctatttt cagctaggtc
     2401 cttctgatta ctgcctacca catctcctga cggctgacgg ctggcagcta cgacgcgacg
     2461 aagctccgtt tgcccgtggc gatctcccag gcgaggtcga ggtggacgcg ggtcccgtcg
     2521 atggtcggga tgggcagggc ctggtcgcgg gccagcagtt cgagttcggc gcaggtgagc
     2581 aggatgccgg cggcaccgcg ccgggccagg ctgcgggcca cctcggcgaa gtcgcggctg
     2641 agggccggag tgacacggcc ccggatgatc tgggtgtaga cgagccggtc gagcgtgcgc
     2701 tgctcggcgt cggtgggcag cagcacgggc acgccgcgct cgcgcaggcc gttgccgtag
     2761 aagcgcgcct gcccggtgag ccgggggccg gccagggcca ggcgcggcag gttgtgggcc
     2821 tgggcatagt cggccacggc gtcgaggata tggtaaatcg gcagggggct atggaccagg
     2881 ggcagcacct cgtagagggc gggcggggcc aggatcgcga aatcggcgcc cgccgcggcc
     2941 acggcctcgc actcctgctc gatctcgtag gccagttctt cccaggcgcc caggtgctgc
     3001 cattgcagat agcgcaggta ttgctggcag ggcgcgcgtc cgccgaccat ctgcggcgcc
     3061 gccccgtcgt cgccggtatc ggcggtgcgg gtcccgattt gttgatcgta gcggaacatc
     3121 gccacgggga aggggccccc gactatgccg atggtcaacg gtcgcgacat gatagctcgt
     3181 atccctccgc aaaagcgccg cctgggtgcg gcggtacatc agtcgagcgg ggccgctgcg
     3241 gcctcgcttg cggcctgccg gatggcggcc agcaacacat cgacccgttc gtgcttggcg
     3301 acgaaggcgc tagcccccgc cgcgcgggcg cggttcgaca gcggcgtttc gtcgtacaac
     3361 gtcaacatga ccacgcgcgt ggccggcact cgcgcccgga tcagttccgc ggtggtg
//
LOCUS       DASUYC010000508         4027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_487342,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000508 DASUYC010000000
VERSION     DASUYC010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487342"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /gene="msrA"
                     /locus_tag="VF276_08350"
     CDS             complement(<1..307)
                     /gene="msrA"
                     /locus_tag="VF276_08350"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX6606909.1"
                     /translation="METQKATFGAGCFWGVEAAFRQVPGVVATTVGYLGGNYKNPTYY
                     DVCSGKTGHAEVVEVVYDPARVAYDDLLTVFWENHDPTTLNRQGPDVGEQYRSAIFFH
                     "
     gene            complement(385..783)
                     /gene="msrB"
                     /locus_tag="VF276_08355"
     CDS             complement(385..783)
                     /gene="msrB"
                     /locus_tag="VF276_08355"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018618843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX6606910.1"
                     /translation="MKPKVIKSDAEWQEQLTPEQYHITRRKGTERAFTGEYAHTHDPG
                     VYTCVCCGAELFSSETKFESGTGWPSFTAPIAEENIRTETDRSLWMRRTEVLCSTCDA
                     HLGHVFDDGPRPTGLRYCLNSASLKLKPAR"
     gene            1207..2928
                     /locus_tag="VF276_08360"
     CDS             1207..2928
                     /locus_tag="VF276_08360"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEX6606911.1"
                     /translation="MSSLTSDRAAGDHRFIPADLPPGDIAALAALYKSLEARPLPDKA
                     ALETWIADWEDLSAVAGELFTEAYVAMTCDTGDPQREATYLHLVEELVPLMERSRFAL
                     QRKLLDTPAVAELDPATYGVYLRAARAQVDLFREENVPLLTEVQKLEQEYEKITGSQM
                     VEFRGKQHTMQEMSVYLEENDRATREEAWRARDAVRAADTAALDDLYDRMIAVRRRIA
                     ANAGKRDYREYAFAGYLRFDYTPEDCLAFHEAIARHVVPVVHEFNERRRALLGVASLR
                     PWDLSVDPEGRPPLRPFTEVDDLIAGCRRIFAQVDPQLAGFYETMVQENLLDLASRQG
                     KAPGGYMVSLQVKRVPFIFMNAVGLKADVDTLLHEGGHAFHYFLARDIPLQSYHETGI
                     EFAEVASQAMEFLARPYLHEFYAADDLARLRDEQIRGSLSFLPFMAMIDAFQHWVYTT
                     ADPVDAAARRAKWAELEARFRLDTDWAGLEHARDNGWQYPHVFTTPFYYVEYGIALLA
                     ALRVWLNSLHDAPAAIEAYKAALALGGSRPLPELFAAAGAEFGLSDGIVRDIVTATVA
                     QIGAEAE"
     gene            3126..3467
                     /gene="trxA"
                     /locus_tag="VF276_08365"
     CDS             3126..3467
                     /gene="trxA"
                     /locus_tag="VF276_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003793764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX6606912.1"
                     /translation="MAETKHVDDATFQNTVLESELPVIVDFWAPWCGPCKMMAPVLEE
                     LSKEYDGKIQFAKLNTDENYDSATQFGIQSIPTLVIFQNGKEVHRLIGFAPKPQLKRQ
                     IDRALGIAQAS"
     gene            complement(3698..>4027)
                     /locus_tag="VF276_08370"
     CDS             complement(3698..>4027)
                     /locus_tag="VF276_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606913.1"
                     /translation="QRHTMDDTGVGEASRVEGDDAGHGRGHEGGLSTGTRYGSSGDHQ
                     GTTDTPGGGPGTTHAHGHGRDTEGAFGEQTPRNNANPHNDFRDAGISKRQVGINEKGD
                     RTQGGDM"
BASE COUNT          729 a         1363 c         1244 g          691 t
ORIGIN      
        1 tgtggaagaa gatggccgag cgatactgct cgcccacgtc gggtccctgg cggttcagcg
       61 tggtcgggtc gtggttctcc cagaacacgg tcaacaggtc atcatacgcc acacgggccg
      121 gatcgtagac cacctcgacc acctcggcgt ggccggtctt gcccgagcag acatcgtaat
      181 aggtggggtt cttgtaattg ccgcccaggt agcccacggt cgtggcgacc acaccgggga
      241 cctggcggaa cgccgcttcc acgccccaga agcagcctgc cccgaaagtt gccttttgcg
      301 tttccatgta ccctgttctc ccttttgcct ccggcgccat aaagaggccg gactatagat
      361 tatacccgat agcgcgccaa tctcctaacg cgccggcttc agcttcagcg aggccgaatt
      421 gaggcaatag cgcaaccccg tgggccgggg gccgtcgtcg aagacgtggc ccaggtgggc
      481 gtcgcaggtg ctgcacagca cctccgtgcg ccgcatccag aggctgcggt cggtctcggt
      541 gcggatattc tcctcggcga tgggcgccgt gaagctgggc cacccggtgc ccgactcgaa
      601 cttggtctcg gagctgaaca actccgcccc gcagcacacg caggtataca cgccgggatc
      661 gtgggtgtgc gcgtactcgc ccgtaaaggc acgctcggtg cccttgcgcc gcgtgatatg
      721 gtactgctcc ggcgtaagct gctcctgcca ctccgcgtcc gacttgatca cttttggttt
      781 catcggtact tcccccataa ttaaaccgga taactatgag accagcacac ggattctgat
      841 cggcagccag gccggtttga aaccggcccc tacaccgcaa aatcaccgtg gccggctact
      901 attgctgctc aatcatcaca cgcaacgctc ggaagcataa acaacgcggg gcgttgtttc
      961 gaggaagccg gatcgggcag cggattcgag aaccatgagg acattccatt gaatatacgt
     1021 agcgcgggcg caaagcgctt tccgccgggc ggcagcgcgg gtggcagggg cacgcgcttt
     1081 ctgatagaat aattgttaac acaatcggcg ccgcttgcgg cgaccatcga ttattcgacc
     1141 gaaccccggc gcgcgtgctg tccacggcca cctggccgcc gggtacggga tcaaaggaga
     1201 accccgttgt ctagcctgac cagtgatcgc gccgccggcg accaccgttt tatccccgcc
     1261 gacctgccgc ccggcgatat tgccgcgctg gccgccctct acaaaagcct cgaagcgcgg
     1321 cccctgcccg acaaggcggc cctggaaacc tggatcgccg actgggagga cctgagcgcg
     1381 gtggccggcg agctcttcac cgaagcctac gtcgccatga cctgcgacac cggcgacccg
     1441 cagcgcgagg cgacctacct gcacctcgtc gaggaactgg tgccgctgat ggagcgcagc
     1501 cgcttcgccc tgcaacgcaa gctgctggac acccccgccg tggccgaact ggacccggcc
     1561 acctacggcg tctacctgcg cgcggcgcgc gcccaggtgg atctgttccg cgaagaaaac
     1621 gtgcccctgc tgaccgaggt gcaaaagctg gagcaagagt acgaaaaaat caccggcagc
     1681 cagatggtcg agttccgcgg caagcagcat accatgcagg agatgagcgt ctatctggag
     1741 gagaacgacc gggccacccg cgaggaagcc tggcgcgccc gcgacgccgt ccgcgccgcc
     1801 gacaccgcgg ccctcgacga cctctacgac cgcatgatcg ccgtgcgccg ccggattgcc
     1861 gccaacgccg gcaagcgcga ctatcgcgag tatgccttcg ccggatatct gcgcttcgat
     1921 tacaccccgg aagactgcct cgccttccac gaggccatcg cccgccatgt ggtgccggtg
     1981 gtccacgagt tcaacgagcg ccgccgggcg ctgctgggcg tcgcaagcct gcggccctgg
     2041 gacctgagcg tcgatcccga agggcgcccg ccgttgcggc ccttcaccga ggtcgacgac
     2101 ctgatcgccg ggtgccgccg catcttcgcg caggtggacc cgcaactggc cgggttctac
     2161 gagacgatgg tgcaggaaaa cctgctcgac ctcgccagcc ggcagggtaa ggcacccggc
     2221 ggctacatgg tctcattgca ggtcaagcgc gtgccgttca tcttcatgaa cgccgtcggc
     2281 ctcaaggccg atgtcgacac gctgctgcac gagggcgggc acgccttcca ctacttcctg
     2341 gcgcgagaca tccccttgca gagctatcac gaaaccggga tcgagtttgc cgaggtggca
     2401 tcccaggcga tggagttcct ggcccgcccc tacctgcacg agttctacgc cgctgacgac
     2461 ctggcgcgcc tgcgtgacga gcagatccgc ggctcgctca gcttcctgcc cttcatggcg
     2521 atgatcgacg cgttccagca ctgggtttat acgacggccg atcccgtgga cgcggcggcg
     2581 cggcgggcca agtgggcgga attggaagcc cggttccgcc tcgacaccga ctgggccggt
     2641 ctcgaacacg cccgcgacaa cggctggcaa tatccgcacg tcttcaccac gccgttctat
     2701 tatgtcgagt acggcatcgc cctgctggcg gcgctgcgcg tctggctcaa ttcgttgcac
     2761 gatgcccccg ccgccatcga agcctacaaa gcggccctgg cgctgggcgg gtcgcgcccg
     2821 ctgcccgagt tattcgccgc agcgggcgcc gagttcggcc tgagcgacgg catcgtgcgc
     2881 gacatcgtca ccgccacggt cgcgcagatc ggcgcggagg ccgagtgagc tagcgggcgg
     2941 cacgggtttt gcacagagac accgcgatta gccgctgtaa gcggcgtgcc catccgccgc
     3001 gggcgggtgc gccggcaatg taacaagcat cactgacggc agagatccgg ctcgcgtata
     3061 ctaaatagag tagtgacacc taccgggcac agtccggcac aaggtgcgaa aggagagtaa
     3121 ttacagtggc agaaaccaag catgtagacg acgccacttt ccagaacacc gttttggaga
     3181 gcgagttgcc tgtgattgtt gatttctggg cgccctggtg tggcccgtgc aagatgatgg
     3241 cgcccgtcct cgaagaactc agcaaagagt atgacggcaa gatccagttt gccaagctga
     3301 acaccgacga gaactacgac tcggccacgc agttcggcat ccagagcatc ccgaccctgg
     3361 tcatcttcca gaacggcaag gaggtccatc gcctgatcgg ctttgccccc aagccgcaac
     3421 tcaagcgcca gatcgaccgc gcgctcggca tcgcgcaagc cagctaaccc ctccgtgggc
     3481 gacgggtcgg caccagccgg ccctaccctc ggaacactga aaacccggcg gatcccgtga
     3541 agggccgccg ggtttttgct tgccccaaaa ctaccatcaa gcgccgcgct gctcgaatcc
     3601 gccgcgacac tcagcaaaca gaacttccta cttatcgtac tgaccactga ctgctgacca
     3661 ctgaccactc gtctcagcac tcagcactca gcactcgtca catgtcgccg ccctgggtgc
     3721 ggtcgccctt ctcgttgatg ccgacctggc gcttcgagat gccggcgtcg cggaaatcgt
     3781 tgtgcgggtt ggcgttattg cgcggggtct gttcgccgaa ggcgccttcg gtgtcccggc
     3841 catggccgtg ggcgtgggtg gtgccggggc cgccaccggg ggtatcggta gtgccctggt
     3901 ggtcgccgct gctgccgtag cgggtgccgg tcgaaaggcc gccttcatgg ccgcggccat
     3961 ggccggcatc atcgccttcc acccggctcg cctcgcccac gccggtatcg tccatggtgt
     4021 ggcgctg
//
LOCUS       DASUYC010000509         2175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1179447, whole genome shotgun sequence.
ACCESSION   DASUYC010000509 DASUYC010000000
VERSION     DASUYC010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179447"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..1213
                     /gene="corA"
                     /locus_tag="VF276_08375"
     CDS             149..1213
                     /gene="corA"
                     /locus_tag="VF276_08375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEX6606914.1"
                     /translation="MQTSTESNQGGEAPARGPTRAQQVRAARAARTAAALPGVAAPRP
                     AVLNIRSVQANGLTWVDIQRPGRAEIEWLRKHYGFHPLHLEDVISKIQRPKLDERDDY
                     IFLVLHWPVYNKITRLTTASEVDIFIGKDYLITIHAGHLRPLLRYFQQCVDDEEVRNR
                     AMRRSSGYLLYRILDKLIDYCFAPLNKIDQNIETIEDMIFADNVRQTVYEISLIRRDI
                     IAFRRVIKPQIGVVASLERRESALAPLLGEDLGEYFSDLSDHMAKIWDTLEDDKDVIE
                     GLSDTNDSLTNTRINDVIKVLTILSVVLLPLTLLSSIYGMNFENLPLSTHPAGFWIAL
                     VAMGIIAAAMLAYFKFRRWI"
     gene            1217..>2175
                     /locus_tag="VF276_08380"
     CDS             1217..>2175
                     /locus_tag="VF276_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="HEX6606915.1"
                     /translation="MLTVRDHLRDNGALRTLGIALGALALGALGGLLVAAANPLIPFA
                     ILIALLPLPWLLTRPQADLWLAIGVITLLPFGTLPFKVGLTPTFLELALLLLAAVSLF
                     AQQRAADPTLRGFVRTPLDSWVLLFLGVISFSFVLGLGRDLSSDIIHNYVKLLLAVTF
                     FFTAGQVLRTPAAIEAVLRVLTLAGGVAALIGIVLWRLPQPTAERLLLLLRPVGYPTS
                     QVLRFVEAGPATGQERAIGTSVDPNSFAGLLVVLVAFTLTQLLGRRPCWPRPVLAGCL
                     LADGAALYLTQSRTAFFAAVAAAGLVSVLRYRKVAVAMVVAGAG"
BASE COUNT          326 a          757 c          715 g          377 t
ORIGIN      
        1 cggccttcgt agcgtgagcc ggaggcttga gcctcggggc actcgcgctt gccaccgggt
       61 ccggcgcagg ggtataatga aggcgccgtg gggccgtggc cgctgcttgc gagggcgacc
      121 ctaccccagc gggagaggag accagccggt gcagaccagc acggagagca atcagggcgg
      181 ggaagccccg gcgcgcggcc caacacgcgc gcaacaggtc cgtgccgcgc gggcggcgcg
      241 caccgctgcg gcgctacccg gcgtggccgc cccgcgcccg gcggtcctca atatccgtag
      301 cgtgcaggcc aatgggctga cttgggtgga catccagcgg cccggtcgcg cggagatcga
      361 gtggctgcgc aagcactacg gcttccaccc gctgcacctg gaagacgtga tcagcaagat
      421 ccagcggccc aaactcgacg agcgcgacga ctacatcttc ctggtgctgc actggccggt
      481 ctataacaag atcacccgcc tgaccacggc cagcgaggtc gatattttca tcggcaagga
      541 ttacctgatc acgatccatg ccgggcacct gcgcccgctg ctgcgctatt ttcagcagtg
      601 cgtcgacgac gaagaggtgc gcaatcgggc catgcgccgc tcgtcgggct acttgctgta
      661 ccgcatcctc gataagctga tcgactactg ctttgccccg ctgaacaaga tcgaccagaa
      721 catcgagacc atcgaagaca tgatcttcgc cgacaacgtg cgccagaccg tgtacgaaat
      781 ctcgctgatc cggcgcgaca tcatcgcctt tcgccgggtg atcaagccgc agatcggcgt
      841 ggtcgccagc ctggagcggc gcgaaagcgc cctggcgccc ctgctcggcg aggacctggg
      901 cgagtatttc agcgacctga gcgaccatat ggcgaagatc tgggacacgc tcgaagacga
      961 taaagacgtg atcgagggcc tgagcgacac gaacgactcg ctgaccaata cccgcatcaa
     1021 cgacgtgatc aaggtgctga cgatcctctc cgtggtcctg ttgccgctga cgttgctgtc
     1081 gagcatctac ggcatgaact tcgagaacct gccgctcagc acgcaccccg cgggcttctg
     1141 gatcgcgctg gtcgccatgg gcatcatcgc ggcggccatg ctggcctact tcaaattccg
     1201 gcggtggatc tagccggtgc tcacagttcg cgaccacctg cgggataacg gggcgctacg
     1261 cacgctgggc attgccctgg gcgcgctggc gctgggcgcg ctcggcgggc tgctggtggc
     1321 cgccgccaac ccgctcatcc cgtttgccat cctcatcgcg ctgctgcccc tgccctggct
     1381 gctgacccgt ccgcaggccg acctctggct ggcgatcggc gtgatcacgc tgctgccctt
     1441 cggcacgttg ccgttcaagg tgggcctcac gccgaccttc ctcgaactgg ccctgctgct
     1501 gctggccgcc gtcagcctgt tcgcgcagca acgcgccgcc gacccgacgc tgcgcggctt
     1561 cgtgcgtacg ccgctcgata gctgggtgct gctgttcctc ggcgtaatca gcttctcgtt
     1621 tgtgctcggc ctgggccgcg acctgagcag cgacatcatc cacaactacg tcaagctgct
     1681 gctggcggtg acgttcttct tcacggcggg ccaggtattg cgcacgcccg cggccatcga
     1741 ggcggtgctg cgcgtgctga cgctggcggg cggggtggcc gcgctgatcg gcatcgtgct
     1801 ctggcggctg ccccagccca ccgccgagcg cctgttgctg ctgctgcgcc ccgtcggcta
     1861 tcccacgtcg caggtgttgc gctttgtcga ggccgggccg gccacaggcc aggagcgcgc
     1921 catcggcacc tccgtggacc ccaacagctt cgcggggctg ctcgtcgtgc tggtcgcctt
     1981 cacgctgacc cagttgctgg gccgccgccc ctgctggccg cgcccggtcc tggccggctg
     2041 cctgctggcc gacggcgcgg cgctctacct gacccagtcg cgcacggcgt ttttcgcggc
     2101 ggtggccgcc gccgggctgg tgagcgtgct gcgctaccgc aaagtcgccg tggcaatggt
     2161 cgtcgcaggg gccgg
//
LOCUS       DASUYC010000510         2220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1275042, whole genome shotgun sequence.
ACCESSION   DASUYC010000510 DASUYC010000000
VERSION     DASUYC010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1275042"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="VF276_08385"
     CDS             complement(<1..1043)
                     /locus_tag="VF276_08385"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX6606916.1"
                     /translation="MSHEDCLQAALAEAQRLGAGYAEARAEDHRVESLTARDTAVERL
                     TSDVDSGWGVRVAVNGAWGFASTADFNLDAARATAAEAVAIAQASATQRLTPLDLSNM
                     PAEQGEYRTAVARDPFAVPLAERIELMLGAVADVRAAHARVHVAGGWLMLWDTQKLFV
                     NTTGAHLAQHIVESGANLYAVALADDGYSYRRQYENVGQAGWEFIEGLRLREEAGRIG
                     REAGDLVVAEWAPDGPTTAVVGSAMMALLVHESCGHPIELDRVLGWEAAFAGTSFLMP
                     DMLGKFHYGSEHVNITADAVTPGGLGTFGWDDEGTPAQVQPIVRGGVFSGYLTSRETA
                     PAIGQRSNGTARAT"
     gene            complement(1120..>2220)
                     /locus_tag="VF276_08390"
     CDS             complement(1120..>2220)
                     /locus_tag="VF276_08390"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6606917.1"
                     /translation="VQGLGAGGIFPIASAVIGDTFPPERRGRALGLLGAVFGLAFIIG
                     PIIGGILLTFSWHWLFLVNLPIAAGLLVVSARLLPATRPAQRRPFDWPGMIVLAVLLS
                     AMTYGLNQIDSARFVQSLTSLDVWPFLLLTVILLPVFWFVERRTSSPVLPVSLFVSRQ
                     VALVAVFALGSGLGEASVVFGPKILKSAFGVTESTASFMLVPLVLTMAIGSPLAGRLL
                     DRLGSRVIVLAGSGLLAAGMITLGLWAHDLILFYVGAGLVGVALGILLGAPLRYIMLG
                     EAPATQRAAAQGALALFTSTGQLIGGAVVGAIAASRGGGVAGYENAYLLVGGLVLLLT
                     TLGFGLKSRAEEAATMHHNVAPPLGEPQQGRA"
BASE COUNT          388 a          817 c          716 g          299 t
ORIGIN      
        1 gtggcgcggg ccgtgccgtt gctgcgctgg ccgatggcgg gcgccgtctc ccggctggtc
       61 aggtagccgg agaacacccc gccgcgcacg atgggctgca cctgtgcagg cgtgccctcg
      121 tcgtcccagc cgaaggtgcc caacccgccc ggtgtcaccg cgtcggcggt gatgttgacg
      181 tgctctgagc cgtagtggaa tttgcccagc atgtcgggca tcaggaacga ggtcccggcg
      241 aaggccgctt cccagcccag cacgcgatcg agttcgatcg ggtggccgca gctttcgtgg
      301 accagcagcg ccatcatcgc cgacccgacc acggcggtcg tcgggccgtc gggcgcccac
      361 tcggccacca ccaggtcgcc cgcctcgcgg ccaatgcgcc ccgcctcctc gcgcagccgc
      421 agcccttcga tgaactccca gcccgcctgg cccacgttct cgtactgccg ccggtagctg
      481 tagccatcgt cagccagggc cacggcgtag aggttggccc cgctttcgac gatatgctgc
      541 gccaggtgcg cgccggtggt gttgacgaac agcttttgcg tatcccagag catcaaccac
      601 ccgcccgcga cgtggacccg agcgtgggcg gcgcgcacat cggccaccgc gcccaacatc
      661 agctcgatgc gctcggccag cggcacggcg aacggatcgc gggccaccgc cgtgcggtac
      721 tcgccctgtt cggcgggcat gttgctgaga tcgagcgggg tcaggcgctg cgtcgcgctc
      781 gcctgcgcaa tggcaacggc ctccgcggcg gtggcgcgcg cggcatccag gttgaaatcg
      841 gcggtcgagg caaagcccca cgcgccattc accgcaaccc gcacgcccca gccgctgtcc
      901 acgtcgctgg tcagccgttc gacggcggta tcgcgggcgg tcagcgactc gacccggtga
      961 tcttcggcgc gcgcctcggc atagcccgcg cccaggcgtt gcgcctcggc cagcgcggcc
     1021 tgcaaacaat cttcgtggct catcggcacc cctacccttc agagaatagc ggcattatac
     1081 acagccgatc cgcctcggtc aaccgtgccg cccgcgccgc taggcgcgcc cttgctgcgg
     1141 ctcgccgagc ggcggcgcca cattatggtg catggtggcg gcctcttcgg cccggctttt
     1201 caggccgaag cccagcgtgg tcagcaacag gaccaggccg cccaccagca ggtaggcgtt
     1261 ctcatagccg gccacgccac caccgcgcga ggccgcgatc gcgccgacca ccgccccgcc
     1321 gatgagctgg ccggtactgg taaagagggc gagcgctccc tgcgccgccg cgcgctgcgt
     1381 cgcgggtgcc tctcccagca taatgtagcg cagcggggcg cccagtagaa tgccaagggc
     1441 cacgccgacc agaccggccc cgacatagaa caggatcaga tcatgtgccc agagtcccag
     1501 ggtgatcatc ccggcggcca gtaacccgct cccggccaaa acgataacgc gtgatcctag
     1561 ccggtccagc aggcgccccg cgagtggcga cccgatggcc atggtcagca ccaacggcac
     1621 cagcatgaag ctcgcggtcg attccgtcac cccgaaggcg ctcttcaaaa tcttaggccc
     1681 gaagacgacc gacgcttcac ccaggcccga tccgagggca aataccgcca ccagggcgac
     1741 ctggcgcgac acgaacagac tcaccggcag gaccgggctg ctcgtgcgcc gttctacaaa
     1801 ccagaagacc gggagcagga tcaccgtcag cagcaagaac ggccagacgt cgagcgaggt
     1861 cagactctgg acaaaccggg cgctatcgat ctggttcagg ccataggtca tggcgctgag
     1921 caacaccgcc agcacgatca tgccgggcca gtcaaacggc cgccgctgcg ccgggcgcgt
     1981 ggccgggagc aaccgcgcgc tcacgacgag cagcccggcg gcgatcggca ggttcaccag
     2041 aaacagccag tgccagctaa aggtgagcag aatgccgccg atgatcggcc cgataataaa
     2101 cgccagacca aagacggccc cgagcagccc gagggcccgg ccgcggcgct ccggcgggaa
     2161 agtgtcgccg atcaccgcgc tggcgatggg aaagatcccg cccgccccca ggccttgcac
//
LOCUS       DASUYC010000511         3414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1904995, whole genome shotgun sequence.
ACCESSION   DASUYC010000511 DASUYC010000000
VERSION     DASUYC010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1904995"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..122
                     /locus_tag="VF276_08395"
     CDS             12..122
                     /locus_tag="VF276_08395"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX6606918.1"
                     /translation="MAVSPDGRWLASGSDDSTVRLWPLAGRAGPASTPTP"
     gene            359..2461
                     /locus_tag="VF276_08400"
     CDS             359..2461
                     /locus_tag="VF276_08400"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX6606919.1"
                     /translation="MPTAVHTPTVWRPLPAPEQGAQPSTEWWLQLLERAFISRAMDDL
                     EVSKEYRPNPDKPLEGKLKFQFSAKGHEIPQLIAAALLNHPHDGATVYYRSRPLMLGV
                     GLSPFEAFASVMHKMEGVSGGRDIGVVFNHRQPGGVTVLPASGDVGAQFTPGVGWAQA
                     VQYRATQMEQREYAGAIALTHAGDGATSTNGFWSALNIAAPLRLPYVMLIEDNHYALS
                     VPWRYQSAAPSIVDNIRAFHNLDIESVEGGDIPALYAALSAAIGRARRGGAQLVHVKV
                     PRLTGHNWQDPAAYKSAEEKADDAHRDPLPRLVAWLQEEHGVSAARIDALREEASAYV
                     REQAEAAWREGHDPAPDDATTHLFALARPVPETPPVTTGARMTMQQAIRQTLEDELAR
                     DPTILVFGEDVGAFSGVHRVSEGLQTHFGEARVFDTSLNEEGIVGRSVGMAMNGLRPV
                     PEIQFRKYADPAHEQIHDAGTLRWRTHGRFGGPIVLRIPVGYQLMGGDPWHAVCGEAV
                     FAHLPGWQIAYPSTVPDAVGLLRTALRGDNPVMFLEHRLLYRLREANAPYPGSDYQLP
                     FGRARRVRAGRHALILTWGEPVYRALEAAGILAAKGVETEILDLRTLVPWDEEAVFEA
                     VRRIGKVLIVHEDNLTCGFGAELAAQISRHAFNYLDGPIYRVAMADVPSPSHKHLFDA
                     IMPTTQRVLAALEELVAW"
     gene            complement(2686..>3414)
                     /locus_tag="VF276_08405"
     CDS             complement(2686..>3414)
                     /locus_tag="VF276_08405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX6606920.1"
                     /translation="PDAAQGSMSDLPIHEPFVLFGYLAGVTRRLELVTSVLILPQRPT
                     ALVAKQAAEIDVLSSGRLRLGAGVGWSSKEYQGLQADFPTRGARMDEQIAVLRALWTQ
                     PVVTFHGRWHHIEAAGINPLPVQRPIPLWIGGMSAAALRRAARWGDGWMPLAPPNDET
                     RARVEQLRAYLQEAGRAPQTFGIEAYLGSPHETPDSRAAQAASWQTLGATHLYASTLG
                     VGCRSPQEHIDALRRIHADISATL"
BASE COUNT          557 a         1206 c         1122 g          529 t
ORIGIN      
        1 cggttcgtag cctggccgtc tcacccgatg ggcggtggct ggcctccggc tcggacgact
       61 cgacagtacg gctctggcca ctggccgggc gggccggtcc ggcttccacc ccgacgcctt
      121 gagataacgc ggagggtgta atgcccgctg ctcgaacctc tgacgataag gaactgttgc
      181 ccttagcgac catacgacta cacgtcgtgc cccgtacaca cgacggctgc cggcgcgttt
      241 caatatacgg agcgcaccta cccaagcaat cgcgcttctg gtatgattaa gaggacgggg
      301 cgctcgccgc tgagcgccgt gaacaccggc cttattcaat gacagaatag gaagacatat
      361 gcccaccgca gtgcataccc cgaccgtgtg gcgtccgttg cccgcgcccg agcagggtgc
      421 ccagccatcc acggaatggt ggctccagtt gctggagcgc gcctttatct ccagggccat
      481 ggacgacctg gaggtcagca aagaataccg gcccaacccc gataagccgc tcgaaggcaa
      541 actcaagttc cagttctcgg ccaagggcca tgagatcccg caactcatcg ccgccgccct
      601 gctgaaccac ccccacgacg gcgcgaccgt ctactaccgc tcgcgcccgc tcatgctcgg
      661 cgtcggtctg tcgccgttcg aggcgttcgc cagcgtgatg cacaagatgg agggtgtgtc
      721 gggcgggcgc gacatcggcg tcgtgttcaa ccaccgccag ccgggcggcg tgaccgtgct
      781 gcccgcttcg ggcgatgtgg gcgcgcagtt cacccccggt gtgggctggg cgcaggccgt
      841 gcagtaccgc gccacgcaga tggagcagcg cgaatacgcc ggggccatcg ccctgaccca
      901 tgccggcgac ggcgccacct cgaccaacgg cttctggagc gcgctcaaca tcgccgcgcc
      961 gctgcgcctg ccctatgtca tgctcatcga ggacaaccac tacgccctgt cggtgccctg
     1021 gcgctaccag tcggcggcgc cctcgattgt cgacaatatc cgcgctttcc acaaccttga
     1081 tatcgagtcg gtcgaagggg gcgacatccc cgcgctctac gccgcgctga gcgccgccat
     1141 cgggcgggcg cgccggggcg gggcgcaact ggtccatgtg aaggtgccgc gtctgaccgg
     1201 gcacaactgg caggacccgg cggcctacaa atcggcggag gagaaagccg acgacgcgca
     1261 ccgcgacccg ttgccgcgcc tcgtcgcctg gttgcaggag gagcacggcg tcagcgccgc
     1321 ccgcatcgac gccctgcgcg aggaggcctc ggcctatgtg cgcgagcagg ccgaagcggc
     1381 ctggcgcgag ggccatgacc ccgcgcccga cgacgccacc acgcacctgt tcgccctggc
     1441 ccgcccggtg ccggagaccc cgccggtcac caccggcgca cgcatgacca tgcagcaggc
     1501 catccgccag acgctggaag acgaactggc ccgcgacccg accatcctgg tcttcggcga
     1561 agacgtgggc gccttctccg gcgtccaccg cgtcagcgag ggcttgcaga cgcacttcgg
     1621 cgaggcccgc gtgttcgaca ccagcctcaa cgaggagggc atcgtcgggc gctcggtggg
     1681 catggcaatg aacggcctgc gcccggtgcc cgagattcag ttccgcaaat acgccgaccc
     1741 ggcgcacgag cagatccatg acgcgggcac gctgcgctgg cgcacgcacg gtcgcttcgg
     1801 cgggccgatt gtgctgcgca tccccgtggg ctaccagctc atgggcggcg acccctggca
     1861 cgccgtctgc ggcgaggccg tgttcgcgca cctgcccggc tggcagattg cctacccgtc
     1921 taccgtgccc gacgccgtgg gcctgttgcg tacggcgctg cgcggcgaca acccggtcat
     1981 gttcctcgaa caccggctgc tttaccgcct gcgcgaagcc aacgcgccct atcccggctc
     2041 cgactaccag ttgcccttcg ggcgcgcccg ccgtgtccgc gcaggtcgcc acgcgctcat
     2101 cctcacctgg ggcgagccgg tttaccgggc gctcgaagcc gccggcatcc tggcggccaa
     2161 aggcgtcgag accgagatcc ttgacctgcg cacactcgta ccctgggacg aggaggccgt
     2221 gttcgaggcg gtgcgccgca tcggcaaggt gctgatcgtc cacgaggaca acctgacctg
     2281 cggcttcggc gccgaactcg ccgcgcagat cagccgccac gccttcaact acctggacgg
     2341 cccgatctac cgggtcgcca tggccgacgt gccctcgccc tcgcataagc acctgttcga
     2401 cgccatcatg ccgaccacgc agcgcgtcct cgccgcgctc gaagaactgg tggcctggta
     2461 accggatctc ctggatacat gcgtaagggc cggggagtcc tctccccggc ccttgctgtt
     2521 tgcggcggtg atgctgatgg gcggcagagc cggtttgaaa ccggccccta caccgccaga
     2581 ttgccgtagc cgatataggg cttccagcat cagtcggaca aaaaacaacg ttaagaaacg
     2641 agcggctgtc gtaatccgac gctccggggg gagggcggtg ggtgcttata gggtcgcgct
     2701 gatgtcggcg tggatccgac gtaaggcgtc aatgtgttct tgcggcgagc ggcagcccac
     2761 gccgagcgtg ctggcgtaga ggtgggtggc gcccaaggtt tgccaactcg ccgcctgggc
     2821 cgcccggctg tcgggcgtct catgcgggga gccgaggtag gcttcgatgc cgaaggtttg
     2881 gggcgcgcgc ccggcctcct gtagataggc gcgcaactgt tcgacccggg cgcgggtttc
     2941 atcattgggc ggcgccaagg gcatccaacc atcgccccag cgtgccgcgc gccgcagcgc
     3001 cgctgcggac atgccgccga tccacagggg aatggggcgc tgcaccggca gcgggttgat
     3061 ccccgccgcc tcaatgtggt gccagcggcc gtggaaggtg acgaccggct gggtccatag
     3121 cgcccgcagc acggcgattt gctcgtccat ccgggcccct cgcgtgggga aatccgcctg
     3181 caagccctga tactccttgg aactccagcc cacgccggca ccgagccgca gccggccgct
     3241 gctcaggaca tcgatctcgg cggcctgctt ggcgacgagc gcggtcgggc gttgcggcag
     3301 aatgagcacg ctcgtcacca gttcgagccg ccgcgtcacg cccgcgagat agccaaacaa
     3361 gacgaacggc tcatggatgg ggaggtcact catggatccc tgcgccgcat cagg
//
LOCUS       DASUYC010000512         3121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1641702, whole genome shotgun sequence.
ACCESSION   DASUYC010000512 DASUYC010000000
VERSION     DASUYC010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1641702"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1207)
                     /gene="dnaG"
                     /locus_tag="VF276_08410"
     CDS             complement(<1..1207)
                     /gene="dnaG"
                     /locus_tag="VF276_08410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX6606921.1"
                     /translation="MANADAINEIKTRLGIVEVVQARTPLQRSGRNYKGLCPFHGEKT
                     PSFVVFPETQSYHCFGCGANGDIFAFVMNTEGLEFRDALARLARQAGVQIAPRDEGEA
                     AADRQRERLYEACAAAALYYHNLLLRSSAEGAKAARAYAQERGLTEATIARFGVGYAP
                     DSWDATANYLRERGFSNAELLEAGLIIEREGENAGYYDRFRHRLVFPIRDLRGHTTGF
                     GARALDDRPPKYMNSPQTPIYDKSATLYGIDQASAAIKAAQCAVIVEGYMDVLIAHQT
                     GHANVVGASGTALTDKQATILKKSARRIVLALDADSAGDLAALKGAEVLEGSMERISV
                     PVLGPRGMLGIERRMDSEIRIMALPRGKDPDELLLQDPAAWDTLLAAAQPVADHFIGV
                     VTGNLDLRTA"
     gene            complement(1269..2345)
                     /locus_tag="VF276_08415"
     CDS             complement(1269..2345)
                     /locus_tag="VF276_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEX6606922.1"
                     /translation="MNYAQVRERIEQEERDRLSPLATKAADSRGRMRPEPPDAIRTAF
                     QRDRDRIIHSKAFRRLKHKTQVFIAPAGDHYRTRLTHTLEVAQIGRTIARALRLNEDL
                     VEAISLAHDLGHAPFGHAGEDALREVYDPNYRHNDQSLRIVDYLARDGQGLNLTYEVR
                     MALPHATPTSEGMSGEGAGQGTLEGKVQKISDSIAYINHDIDDAIRAGIITEADLPRR
                     CNAVLGGSHSERINTLVCNLVEVNWDVAYPPPGARLPSPDSVDIHFSPDVLAAADELK
                     AFLFERVYVGSAAKAEEERVHGMIRALYEHYCAHFDEIPAEMARNPRDEPRERIVVDY
                     IAGMTDRYAIQKFSELYVPRLWSI"
     gene            complement(2791..>3121)
                     /locus_tag="VF276_08420"
     CDS             complement(2791..>3121)
                     /locus_tag="VF276_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606923.1"
                     /translation="TQLVSSFVVQDECARANADVQALEQALREFAQTVVPASPEVDAL
                     AARLTAEAGLKPGDSRHIACAVLAGASDFLSCTPRRINKQPAVEAVLGHAFPMWSPSD
                     FLREVLV"
BASE COUNT          481 a          980 c         1099 g          561 t
ORIGIN      
        1 gcgccgtgcg caggtcgaga ttgccggtga cgacgccgat gaagtggtcg gcgaccggct
       61 gggcggcggc gagcagggta tcccaggcgg cggggtcctg caacagcagt tcgtcggggt
      121 ctttgccgcg cggcagggcc atgatgcgga tctcggagtc catgcgccgc tcgatgccga
      181 gcatcccgcg cgggccgagc acgggcacgg agatgcgctc catgctgcct tcgagcacct
      241 cggcgccctt gagcgcggcc aggtcgcccg cgctgtcggc gtcgagcgcc agcacgatgc
      301 ggcgggccga cttcttgagg atggtcgcct gcttgtcggt cagcgcggtg cccgacgcgc
      361 cgacgacgtt ggcgtggccg gtctggtggg cgatcagcac gtccatgtag ccctcgacga
      421 tcacggcaca ctgggcggcc ttgatcgcgg cggacgcctg gtcgatgccg tagagcgtgg
      481 cgctcttgtc gtagatcggc gtctgcggcg agttcatata cttgggcggg cggtcgtcga
      541 gggcgcgggc gccgaagccg gtggtgtggc cgcgcaggtc gcggatgggg aagaccaggc
      601 gatgacggaa gcggtcgtaa tagcccgcgt tctcgccctc gcgctcgatg atcagcccgg
      661 cttcgagcag ttcggcgttg ctgaagccgc gctcgcgcag gtagttcgcg gtggcgtccc
      721 agctatcggg cgcgtagccg acgccgaaac gggcaatcgt cgcctcggtc agtccgcgct
      781 cctgggcata ggcgcgggcc gctttagcgc cctcggcgga cgagcgcagc agcaagttgt
      841 gataatagag cgccgccgcc gcgcacgcct cgtacagccg ttcgcgctgg cggtcggcgg
      901 ccgcttcgcc ttcgtcgcgc ggggcgatct gcacgcccgc ctgccgggcc agccgggcca
      961 gcgcatcgcg gaactccagc ccctcagtgt tcatcacgaa ggcgaagatg tcgccgttgg
     1021 cgccgcagcc gaagcagtgg tagctctgcg tctcggggaa cacgacgaac gacggcgtct
     1081 tctcgccgtg gaaggggcag aggcccttgt agttgcggcc gctgcgctgc aacggggtac
     1141 gcgcctgcac cacttcgacg atgcccagcc gcgtctttat ttcgttgatc gcgtctgcat
     1201 tagccataca aatgctgagt cctgagcgcc ggtactacgg tgtaactcgc gcgtcgcggc
     1261 atagtgcgct agatcgacca gagccgcggc acgtagagtt cgctgaactt ctgaatggcg
     1321 tagcgatcgg tcatgcccgc gatgtaatcg acgacgatgc gctcccgcgg ctcgtcccgc
     1381 gggttgcggg ccatctcggc gggaatctcg tcaaagtgag cgcaataatg ctcgtagagc
     1441 gcgcggatca tgccgtggac acgctcttcc tcggccttcg ccgccgaccc gacgtagacc
     1501 cgctcgaaca ggaaggcttt caactcgtcg gcggcggcca gcacgtcggg cgagaaatgg
     1561 atatcgacgc tgtcggggct gggcagccgc gcgcccggtg gcggataagc cacgtcccag
     1621 ttgacctcga ccaggttgca gaccagcgtg ttgatgcgct cggagtggct gccgccgagc
     1681 acggcgttac agcggcgcgg caagtcggct tcggtgataa tgccggcccg gatcgcgtcg
     1741 tcgatatcgt ggttgatata ggcgatgctg tcgctgattt tctggacctt gccttcgagg
     1801 gtgccctggc ccgcgccttc gccgctcatg ccctcgctgg tgggggtggc gtggggcaag
     1861 gccatgcgca cttcatacgt caggttcaga ccctgcccgt cgcgcgccag gtaatcgaca
     1921 atgcgcaggc tctggtcgtt atgccggtag ttggggtcgt acacttcgcg cagcgcatcc
     1981 tcgcccgcat ggccgaaggg ggcatggccc aggtcatgcg ccagggaaat ggcctctacc
     2041 aggtcttcgt ttagacgcag cgcgcgggca atcgtccggc caatctgggc gacttccagc
     2101 gtgtgggtga gccgcgtccg ataatggtcc cccgcggggg cgatgaagac ctgtgtcttg
     2161 tgcttgaggc ggcgaaacgc ttttgagtga atgatccggt cgcggtcacg ctggaacgcc
     2221 gtgcgaatgg catccggggg ctccgggcgc atcctgccgc gcgaatcggc tgctttggtc
     2281 gccaacgggg agagacggtc acgctcttcc tgctcgatgc gttcgcgaac ctgggcgtag
     2341 ttcatcaaat ggctaagttc gcctctccaa gagctgatcg ccgtcacaac gacactccag
     2401 tatagcatat cccccgatgg tctgtatagg gggtagggcc aatcgggcca attgcccggc
     2461 ctgttccggt tgcggtatcg tgcgccggat agggcgattc gcctggtgcg cgcggcctta
     2521 tccggccggg tgcctgtata aacctggata gcggggagtg aaaacatcgc attatagcac
     2581 cgattacctt accaaatacc ttaccaaacc gccgcgcggc aaattgtgag gaaatcgtga
     2641 cctgccgggc gagcgcggca gttacgcaga ggccaacggg aatcttgcgt tgtaggggcc
     2701 ggtttcaaac cggccctgcg acgcgggcgc ccccctttgg cgctctgccg attgtagggg
     2761 ccggtttcaa accggcctgc cggcggcccg ctagacgagc acttcgcgca ggaagtcgct
     2821 ggggctccac atcgggaagg cgtggccgag cacggcttcg acggcgggct gcttgttgat
     2881 gcgccggggc gtgcaggata ggaagtcgct ggccccggcc agcacggcgc aggcgatgtg
     2941 ccggctgtcg cccggcttga ggcccgcttc cgccgtgagc cgggcggcca gggcgtccac
     3001 ttccggcgag gccgggacga cggtctgggc gaactcgcgc agggcctgct ccagcgcctg
     3061 cacatcggcg ttggcccgcg cgcactcgtc ctgcacgacg aaggacgaga ccagttgcgt
     3121 g
//
LOCUS       DASUYC010000513         2504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_753469,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000513 DASUYC010000000
VERSION     DASUYC010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753469"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            441..>2504
                     /locus_tag="VF276_08425"
     CDS             441..>2504
                     /locus_tag="VF276_08425"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX6606924.1"
                     /translation="MNGNTPPASAPTRRPAHPLAPDFLSLFGDAAVVRWAIQLRWWML
                     GAMLGLGLLLTLTGALTQQMYINVLLLALPVAGYNLVFLILLHLAPLGRTPAPLIRLR
                     WLQAPPDMIAISVAIHLSGGATTPFSIFYVLFILTAVVFTPRYGTYVAALESALCYTA
                     LVVLELTIWPPPANPLLAPEQIAGQTRQFIYGWHLLTLVFILGAGAYMADRLGRRILT
                     GEEIIQRQVGDLTLLYRFGDNLSAVPNLEQALQYIVTELSAILDAADCSLVLLNERNE
                     AEIRAAVGIPPQALIAYRKRVLDQSNPLLASMLSSGIGMFAPDIDEVPGLRDLVARPA
                     QSFYSFPLRTEERMLGMINFSFTRPYTMPPSTYDLVAACSRQASMAIERTLLYQEAQR
                     AAREMSSLYHIGLAASSSLEINSVLSQIADQVQTLLAPDSLLLAFYDDTRQLLDFVLH
                     RADGRIWPRETLPVAQAGSAGWIVAHQQPLLIRDRDKELPDVGLASNAGAEGQSLVGV
                     PLLTNNRVIGVLSIQKREPAAYGDDALRLLSAIAAQAALALENARLHEATREQALRDS
                     LTGAYNHAALLDNITRGVEHAAARGAPVALIMLDIDLFKKYNDTYGHMAGDDALRTVV
                     TAISQNIKSTDSVGRWGGEEFGVLLPGAACAQALQVAYRIRATLARIEMPVHNGTRLP
                     VPTVSQ"
BASE COUNT          391 a          896 c          755 g          462 t
ORIGIN      
        1 cgggcgcctt cccggatctg cgcctggtgc cagatccggc agttaggtcc gaggtcggcg
       61 cccgccgcca cctccgccgt cggatgcacc cgcacgttct ctgcaagcac cacactagcc
      121 tctttcctaa ttcgcgtata ccacaaacag tcaattatac catctccgtg cgcgccggca
      181 aagggcggga gtccgccgcc gtttgacaca ccgggatggc tagtgtagaa tgaccgcagc
      241 ccaattgccg ccggcgcgcc cgctcttgcc gtgagagtgc gagtctctct tgcctgaccc
      301 tctgctgatc gccggctcct gctggaatac cgttgcctgc cgtccacgcc cctgccgcga
      361 acggtaggcg ggatctccgc tttcttcgga gcgctgcatg ccaacggcca cctcgtctgg
      421 tttacgcgtg atctgccccc atgaacggta ataccccccc ggcgtccgcg cccacccggc
      481 gccccgccca cccgctggcc cccgactttc tctccctgtt cggggatgcc gccgtcgtgc
      541 gctgggccat ccaactgcgc tggtggatgc tgggggcgat gcttggcctg gggttgctgc
      601 ttacgttgac cggggcgctc acgcagcaga tgtatatcaa cgtgctgctc ctcgccctcc
      661 cggtggccgg ctataacctc gtcttcctca tcttattgca tctggcgccg ctcgggcgga
      721 ccccagcccc tcttattcgg ctgcgctggt tgcaggcccc gcccgacatg atcgccatca
      781 gcgttgccat ccacctgtcg ggcggcgcga ccacgccgtt tagcatcttc tacgtgctgt
      841 tcattcttac cgccgttgtt ttcacgccgc gctacggcac ctatgtcgcc gcccttgaat
      901 ccgcgttgtg ctataccgcc ctggtcgtgc tggaactcac gatctggccg cccccagcaa
      961 accccttgct ggctcccgag cagatcgccg gccagacccg gcagttcatc tatggctggc
     1021 acctgctgac cctggtgttt atccttggtg ccggcgccta tatggcagac cggctgggcc
     1081 ggcggatcct caccggtgag gaaattatcc agcgtcaggt gggcgatctc acgctgctct
     1141 accgcttcgg cgataacctg agcgcggtgc ccaacctgga acaggccttg caatacatcg
     1201 tcaccgagtt gagtgccatc ctggacgccg ccgactgctc gctggtcctg ctcaacgaac
     1261 gcaacgaggc cgagatccgc gcggcggtgg gcatcccgcc ccaggcgctg atcgcctacc
     1321 gcaagcgcgt gctcgatcag agcaacccac tgctcgcctc gatgctgagc agcgggatcg
     1381 gcatgttcgc ccccgatatt gacgaggttc ccggcctgcg cgacctggtg gcccggcccg
     1441 cgcaatcctt ctactccttt cctctgcgca ccgaagagcg gatgctgggc atgatcaatt
     1501 tcagcttcac ccgcccctac actatgccgc ccagcacgta tgacctggtg gcggcgtgca
     1561 gccggcaggc gagcatggcg atcgagcgca cgttgctcta ccaggaagca cagcgcgccg
     1621 cccgcgagat gtccagcctc taccatatcg gcctggcggc gtcgtcgagc ctggagatca
     1681 acagcgtgct cagccagatc gccgaccagg tgcaaacgct gctcgccccg gactcgctgc
     1741 tgctcgcctt ctacgacgac acgcgccaac tgctcgattt tgtcttgcac cgcgccgacg
     1801 ggcgcatctg gccccgtgaa acgctgccgg tggcccaggc ggggagcgcg ggctggattg
     1861 tggcccacca acaaccgctc ttgatccgcg atcgcgacaa ggaactgcct gatgtgggcc
     1921 tggcgtccaa tgcgggcgcc gaggggcaat cgctcgtcgg cgtgccgctg ctgaccaata
     1981 accgcgtcat cggcgtcctc tccatccaga agcgcgagcc ggcggcgtac ggcgacgacg
     2041 ccctgcgttt gctgtcggcg attgccgccc aggcggcgct ggccctcgaa aatgcccggc
     2101 tgcatgaagc gacgcgcgag caggcgctcc gcgacagctt gaccggcgcc tacaatcacg
     2161 ccgcgctgct cgacaacatc acgcggggcg tggaacacgc ggcggccagg ggcgcgccgg
     2221 tggcgctgat tatgctcgat atcgacctgt tcaagaagta caacgatacc tacggccata
     2281 tggccggcga cgacgcgctg cgcaccgtgg taacggcgat cagccagaat attaagagca
     2341 ccgacagcgt gggccgctgg ggtggggaag agttcggcgt gctgttgccc ggcgcggcgt
     2401 gtgcccaggc gctgcaggtg gcctatcgca tccgcgccac gctggcccgc atcgagatgc
     2461 cggtccacaa cggcacccgc ctgccggtgc ccacggtcag ccag
//
LOCUS       DASUYC010000514         2703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1792694, whole genome shotgun sequence.
ACCESSION   DASUYC010000514 DASUYC010000000
VERSION     DASUYC010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1792694"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="VF276_08430"
     CDS             <1..948
                     /locus_tag="VF276_08430"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEX6606925.1"
                     /translation="DLTGSRPVDHSTPPEYDVVIAGDVYCDLIFTGLDTLPRLGEEHF
                     ARAFEMTPGGVFITAAALARLGLSVGLCSWLGDDPFSEFALRCFAQEGLSTALVQRRP
                     GPLPTLTVSLSLPGDRSFVTYRAPRPSPDPAAVLRRTTFRHLHISSLPGLFEHPDLVE
                     LAHERGASISIDCQWCPDLIAAPDLADRLVAARPAVFLPNLDEALAITHTTAATEALE
                     SLAAWVPTVITLGPAGVLAGCDGERYAVPALPVTAVDTTGAGDAFDAGFIYGLLHGLP
                     FGECLRVGTVCGGLSTTAHGGATAAPRRPVVEAWLARGW"
     gene            1005..2330
                     /locus_tag="VF276_08435"
     CDS             1005..2330
                     /locus_tag="VF276_08435"
                     /inference="COORDINATES: protein motif:HMM:NF023401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEX6606926.1"
                     /translation="MNPLKLAYIGGGSAYAAGVIRSFANAALRDPEAFADGEIALMDL
                     DAGNLETVSALGRRMVAAAGAPLRITATTDRERALDGATFVLTSFRPGGLKARALDER
                     IPLDFGILGQETVGPGGFFFGLRSLQVMRDLCAEMDRLCPDAWLLNYTNPTNIIGEAV
                     SRYTKLNVLALCDGGKHDAYHIAGLLGYRPDQVEVRGLGLNHATWSTRFLLDGQDGIA
                     LLREAAPRLLADDSVIPKAKRMIRLALRYGRLPNEYMQYYYYPQETVAEARAAAEAGH
                     TRAEAIMAELPDIFAHYREQADQPEPHLTRQRGGGGFGDFAVEILRAILCDEGRVEML
                     NVPNLGTLPGQPPERVAEVPCRIDRHGATPLAQGPLPTELHGLIAALADYQALAATAG
                     WEGDARSATHALAANPLCWHLDADQVDALYARLAQAHAAWLPKRLLPAA"
     gene            2395..>2703
                     /locus_tag="VF276_08440"
     CDS             2395..>2703
                     /locus_tag="VF276_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606927.1"
                     /translation="MDTLNEAPHDSEGNEAPAWTEWRERVASPDDLAACVEALGLLPL
                     RAEAPWPALWRMLAPGTTTTTAWAWVGTLIARRQIFAGRVLPGLESVCLTSLPVFALA
                     F"
BASE COUNT          361 a         1009 c          931 g          402 t
ORIGIN      
        1 gatctgacgg gtagcaggcc agtggatcat tcgacgcctc ctgaatacga cgtggtgatc
       61 gcgggcgacg tgtactgcga tctgatcttc accggcctcg acacgctgcc gcgactgggc
      121 gaggagcact tcgcccgcgc cttcgagatg acgcccggcg gcgtgttcat caccgccgcc
      181 gcgctggcgc gcctgggctt gagcgtgggt ctctgctcct ggctcggcga cgacccgttc
      241 agcgagtttg cgctgcgctg cttcgcccag gagggcctgt ccaccgcgct cgtgcagcgc
      301 cggcccggcc cgctgcccac cctgaccgtc tcgctctcgc tgcccggcga ccgctccttc
      361 gtcacctacc gggcgccgcg gcccagcccc gatcccgccg ccgtgctgcg ccggaccact
      421 ttccgccatc tgcacatttc gtcgctgccg gggctcttcg agcaccccga cctggtggaa
      481 cttgcgcacg agcgcggagc cagtatttct atcgattgcc agtggtgccc cgacctgatc
      541 gccgcgcccg acttggccga ccggctggtg gcggcgcgcc cggcggtgtt cctgcccaac
      601 ctggacgagg cgctggcgat tacacacacg acggcggcca ccgaggcgct ggagagcctg
      661 gccgcctggg tgcccacggt gatcaccctg ggcccggcgg gggtgctggc cggatgcgac
      721 ggcgagcggt atgccgtccc ggcgctgccc gtcaccgcgg tggacacgac cggcgcgggc
      781 gatgccttcg acgccggatt tatctacggc ctgctacacg gcctgccctt cggcgaatgc
      841 ctccgcgtgg ggacggtctg cggcggcctt tcgaccacgg cccacggcgg cgcgaccgcg
      901 gccccgcgcc ggcccgtggt ggaggcgtgg ctggcccgcg gctggtagcg gtcggcctgg
      961 ggcgggccgg tttcaaaccg gcccctacat gggaaaggcg cactatgaac cctctgaaac
     1021 tggcctatat cggcggtggc agtgcctatg ccgcgggcgt cattcggtcc ttcgcgaacg
     1081 ccgccctgcg cgaccccgag gctttcgcgg acggcgagat cgcgctgatg gatctggacg
     1141 ccgggaacct ggagacggtg tcggccctgg gacggcgcat ggtggcggcg gccggggcgc
     1201 cgctgcgcat cacggcgacc accgaccgcg agcgcgccct cgacggcgcg acctttgtgc
     1261 tgacctcgtt ccgccccggc ggactgaagg cgcgcgccct ggatgagcgc atcccgctcg
     1321 acttcggcat cctgggccag gagacggtgg ggccgggcgg cttcttcttc ggcctgcgca
     1381 gcctccaggt gatgcgcgat ctctgcgccg agatggaccg cctctgcccc gacgcctggc
     1441 tgctcaacta caccaacccg accaacatca tcggcgaggc ggtgagccgc tacacaaagc
     1501 tgaacgtgct ggcgctgtgc gacggcggca aacacgatgc ctatcacatc gcgggcctac
     1561 tgggctaccg ccccgatcaa gtcgaggtcc gcggcctggg cctgaaccac gccacctggt
     1621 ccacgcgctt cctgctcgac gggcaggacg gcatcgccct gctgcgcgag gcggccccgc
     1681 gcctgctggc cgacgactcg gtcatcccga aggccaagcg catgatccgc ctggcgctgc
     1741 gctacgggcg cctgcccaac gagtacatgc agtactatta ctacccgcag gagacggtgg
     1801 ccgaggcgcg cgccgccgcc gaggccgggc acacacgcgc cgaggcgatc atggccgaac
     1861 tgcccgacat cttcgcccac taccgcgagc aggcggacca gcccgaaccc cacctgaccc
     1921 gccagcgcgg cggcggcggc ttcggcgact tcgcggtcga gatcctgcgc gccatcctgt
     1981 gcgacgaggg gcgcgtggag atgctgaacg tgcccaacct gggcacgctg cccggccagc
     2041 cgccggagcg cgtggccgag gtgccctgcc gcatcgaccg gcacggcgcc acgccgctgg
     2101 cccaggggcc gttgcccacc gaactccacg gcctgatcgc cgccctggcc gactaccagg
     2161 cgctggccgc cacggccggc tgggaaggcg acgcccgcag cgccacgcac gcactggctg
     2221 ccaacccgct ctgctggcac ctggacgccg atcaggtgga cgcgctctac gcccggctgg
     2281 cccaggccca cgccgcgtgg ctgccgaaac gcttgctccc tgcggcatag ccgccgcccc
     2341 agcggcctgc aacccgcata ccctcgcgtg cgtatctctg gtataatgcg cgcgatggat
     2401 accctgaacg aagcacctca cgatagcgag gggaacgagg cgcccgcctg gaccgagtgg
     2461 cgcgaacgtg tggcctcgcc ggacgacctg gcggcttgcg tcgaggcgct gggcctgctg
     2521 ccgttgcggg cggaagcgcc ctggcccgcc ctgtggcgga tgctggcgcc cggcaccacg
     2581 accaccacgg cctgggcctg ggtcggcacg ctgatcgccc ggcggcagat cttcgccggg
     2641 cgggtgctgc cggggctgga gagtgtctgt ctcacctcgc tgccggtgtt cgcgctggcg
     2701 ttt
//
LOCUS       DASUYC010000515         5273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_26795,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000515 DASUYC010000000
VERSION     DASUYC010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5273
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26795"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1237
                     /locus_tag="VF276_08445"
     CDS             <1..1237
                     /locus_tag="VF276_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HEX6606928.1"
                     /translation="DIILCYVGTYATSSQVLPAVQRPNRPVLVLNLQPVAALAYEHTD
                     TGEWLANCCACCVPEISGAFRRCGIHFNVVSGLLREEDGPAGAHAWAEIRDWLAAAAV
                     LRSLQYGRVGFLGHTYPGMLDMYSDFTMITGQTGLHVEILEMDDLKQRVDAARPAEID
                     AVLDRTRDLFEISEDSPSDPLARAPHPDDLRWSAQVAVGLDTLRRDFALDALSYYYRG
                     LDGNEDEQLAAGLILGNTLLTGQGVPCSGEGDTKNAIAMLIMDRLGAGGSYTEFYAMD
                     FVDDFCLMGHDGPFHMGISEGKPVLRGLALYHGKRGHGVSVEARVKTGPITILTVTQT
                     AAGKLKLIVAEGEAVPGPVLRIGNTNSRLKFALPPAQFMNDWCEQGPTHHCALGIGRQ
                     AAKLEKLGRLLGLEVARVC"
     gene            1313..>5273
                     /locus_tag="VF276_08450"
     CDS             1313..>5273
                     /locus_tag="VF276_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606929.1"
                     /translation="MEDAARVRIDDLARQFASPPREYSPAPIWWWSGERLERDRLRRQ
                     LEQLAAGGIYNVVLLNLAPTGPLFGSVADDPPFLSEEWWAIFLGVCADARELGVRILF
                     YDQIGFSGANLQGVLARANPQFTGHWLACATVEGEGSLELATPGEGTALGAVVTDLAS
                     GKREVVPLTERGVTYTAPAPSRLRLVYAVPRGFDYFNPAACQALFDTVHYEFERRAGQ
                     FFGTVIAGSFQDELPSMPTWGPDFAAAFRERCGYDLAENLGALWEGEDDWARRVRADY
                     QATRAALAERAFFKPLFDWHARHSLICGFDQTHPARAGDPQGAVGLYADYMRTHRWYT
                     APGSDHHGEAKIHSSLAHLYDRPRTWIESFHSSGWGGTLEETFDWLLPWLRAGANLYN
                     PHAVYYSTRGGWWEWAPPSTCWRQPYWRHYRQFAGAVNRLCYMLTRGHHVCDIGILYP
                     TTTVQSAYTPDGPLPSAQPAHLAYIDLVGHMTWYHTVPGVFDRDRRDFDVLDDASIQR
                     AQIADGALVIGAERYSVVVLPTCTVLEPATAAALCRFVEAGGRLIAVGALPEAVAGPD
                     PAPVGALRGLFNAGRATLVPRPDDVPAALADLPRQVDAPVPVLHRRIGNSDLVFVPAA
                     FPRATQEPANKHWANFHYTFDPADYQRRMRVRVRGVSGAPQLWDAATGQRRALAATPV
                     ADGVEVEIPFDDGPAALLVWADPAAPAEQVSGVNAAGSEETLATLDDWQSAIVPTLDN
                     RYGDLALPAAPGAPPVQTWRFAHRIERPGEDSQAADPTDAGDWQPVEATFGPYGWWTG
                     PLPADRLPPPLADRADGQPLAPTDWHPAVYSRSRGIARDPIHYSTLGPKARVPQEFLL
                     FGPTAPGEGVRFRTSVASPAEQRAYLALGGPGAKRAWLNGEPCGTAPAGYLWLAPVTL
                     RQGRNVLEWELVAEARVNLRASWALVRDPARYARPEWLTSAGAPVRDSRLRFSTTLDL
                     PFAPVEGTIQIGTADPCRVIVNGVEVGRQGGFDPYLVGRVQPYTTDAWRQGANSVVLE
                     VLDPGRPVALVADALIHSADGETAGLMTGADWWVQRDDGPAQPATLRRRQWIDQPAID
                     ASEGFGEMDVAFANLWRRPHPLPDADWLEDTPHDDTVLPLVPDAFGSRRVEWFRWQIP
                     PGATAIHLPVDGEVRLWVDGAEVEVSEDTVKQARLGGAPAASRQVVMRVVPASNLSGG
                     AVFAGPVTYEMGVGRIRPGEWAAQGLEAYSGGVRYSTAFALVAPPTGRLMLDLGRVRG
                     TAEVTVNGQSCGVRVWSPYRFDITAAAQVGQNTVEVLVCNTLAPYLRAVSPTHYVFPG
                     "
BASE COUNT          742 a         1936 c         1825 g          770 t
ORIGIN      
        1 cgatatcatc ctctgctacg tcggcaccta tgccacgtcc tcccaggtcc tgccggcggt
       61 gcagcgtccg aacaggccgg tgctggtgct caatttgcag cccgtggccg ccctcgctta
      121 tgagcacacc gatacgggcg agtggctggc gaactgctgc gcctgctgcg tgcccgagat
      181 ctcgggcgcg ttccggcgct gcggcatcca tttcaacgtg gtgagcgggc tgctgcggga
      241 agaggacggc ccggcgggcg cgcacgcctg ggccgagatc cgcgactggc tggcggcagc
      301 tgccgtgctg cgctcgttgc agtatggccg ggtcggcttc ctcggccaca cctatccggg
      361 catgctcgat atgtattccg acttcacgat gatcaccggg caaacgggcc tgcacgtcga
      421 gatcctggag atggacgacc tcaagcagcg tgtggacgcc gcccgcccgg cagagatcga
      481 cgccgtgctc gaccgcacac gcgacctgtt cgagatcagc gaggacagcc cgtccgaccc
      541 gctggcccgc gccccgcacc ccgacgattt gcgctggtcg gcccaggtgg ccgtcgggct
      601 ggatacgctg cggcgcgact tcgccctgga cgccttgagc tactactatc gcggcctcga
      661 cggcaacgag gacgagcagt tggccgccgg gctgattctc ggcaacaccc tgctgaccgg
      721 ccagggggtg ccctgctcgg gcgaaggcga cacgaagaac gccatcgcca tgctgatcat
      781 ggaccgcctg ggcgccgggg gatcctacac cgagttctat gccatggact tcgtggacga
      841 tttctgcctg atgggccacg acgggccgtt ccacatgggc atctccgaag gcaagccggt
      901 cttgcgcggg ttggcgctgt accacggcaa gcgcgggcac ggcgtgtcgg tcgaggcgcg
      961 cgtcaagacc ggcccgatca ccatcctcac cgtcacccag acggcggcgg gcaagctcaa
     1021 actgatcgtg gccgagggcg aggcggtgcc cggcccggtg ctgcgcatcg gcaacaccaa
     1081 ctcgcggctc aagttcgcgc tgccgcccgc ccagttcatg aacgactggt gcgagcaggg
     1141 gcccacccac cactgcgcgc tcggcatcgg gcgacaggcc gcgaagctgg agaaactggg
     1201 ccgcctgttg ggcctggagg tagcccgcgt ttgttaggcg ccggtgcccc gcgccgccac
     1261 cggatgcgcg ggccggcagt tcgccccacc gaagatccct ggaggtagcc tgatggagga
     1321 tgcagcgcgc gtgcgcattg acgacctggc ccggcagttt gcctcacccc cgcgcgagta
     1381 ttccccggcg cccatctggt ggtggagcgg cgagcggctg gagcgcgacc gcctgcgccg
     1441 gcagttggaa caactggcgg cgggcggcat ctacaacgtg gtgctgctca atctggcgcc
     1501 caccgggccg cttttcgggt ccgtggccga cgatccgccc ttcctctcgg aggagtggtg
     1561 ggcgatcttt ctcggcgtct gtgccgacgc acgcgaactc ggcgtgcgga ttctcttcta
     1621 cgaccagatc ggcttctccg gcgcgaacct gcagggcgtc ctggcccgcg ccaacccgca
     1681 gttcaccggc cactggctgg cctgcgctac cgtcgaaggc gaaggctccc tggaactggc
     1741 gacccccggc gaagggacag ccctcggcgc cgtcgtcacc gacctggcga gcggcaagcg
     1801 cgaggtagtg ccgctcaccg agcgaggcgt cacgtacacg gcgccggccc cgtcccggct
     1861 gcggctggtg tatgccgtgc cgcgcgggtt cgactatttc aatcccgccg cctgccaggc
     1921 gctgttcgac acggtgcatt acgagttcga gcggcgcgcc ggccagttct ttggcacggt
     1981 catcgccggg tccttccagg atgagttgcc gtcgatgccg acctggggtc ccgacttcgc
     2041 ggcggccttc cgcgagcgct gcggctacga cctcgcggaa aatttgggcg cgctctggga
     2101 gggcgaggac gattgggcac gccgggtgcg cgccgactat caggccaccc gcgcggccct
     2161 ggcggagcgg gcgtttttca agcccttgtt cgactggcac gcccgccata gcctgatctg
     2221 cggcttcgac cagacccacc cggcccgtgc cggcgatccg cagggcgcgg tgggcctcta
     2281 cgccgactac atgcgcaccc accgctggta cacggcgccc ggcagcgacc accacggcga
     2341 ggccaagatc catagctcgc tggcccacct ctacgaccgg ccgcgcacct ggatcgagtc
     2401 cttccatagc agcggctggg gcggcacgct cgaagaaacg ttcgactggc tgctgccctg
     2461 gctgcgcgcc ggcgccaacc tctataaccc gcacgccgtc tactacagca cccgcggcgg
     2521 ctggtgggag tgggcgcccc cgtccacctg ctggcgccag ccctactggc gccactaccg
     2581 ccagtttgcc ggggcggtaa accgcctctg ctacatgctg acgcgcggcc accatgtctg
     2641 cgacatcggc atcctgtacc cgacaaccac cgtccagtcg gcctacacgc ccgacgggcc
     2701 gctaccgtcc gcgcagcccg cccacctggc ctacatcgac ctggtcggcc acatgacctg
     2761 gtatcacacg gtgcccggcg tattcgaccg cgaccggcgc gactttgatg tgctggacga
     2821 cgcctcgatc cagcgcgccc agatcgcaga cggcgcgctg gtaatcggcg cggagcggta
     2881 tagtgtagta gtgctgccca cctgcacggt actcgaaccg gcgaccgccg ccgcgctctg
     2941 ccggttcgtt gaagccggcg gccggctcat cgccgtgggc gccctacccg aagcggtggc
     3001 cggccccgac ccggcgccgg tgggggcgct gcgcggcctg ttcaacgcgg gccgggccac
     3061 gctggtcccg cgcccggacg atgtgcccgc cgcgctcgcc gatctgccac gtcaggtgga
     3121 cgcgcccgtg ccggtgctgc accggcgcat cggcaacagc gacctggtgt tcgtgcccgc
     3181 ggccttcccc cgcgccaccc aggaaccggc caacaaacac tgggccaact tccactacac
     3241 cttcgacccc gccgactacc agcggcggat gcgcgtccgg gtgcgcggcg tcagcggcgc
     3301 gccccaactc tgggacgccg ccaccggcca gcggcgcgcg ctcgccgcca cgcccgtcgc
     3361 cgatggggtg gaggtggaga tccccttcga cgacggcccc gcggccttgc tggtctgggc
     3421 cgatcccgcc gcgccggcgg aacaggtgtc cggcgtgaac gccgccggca gcgaggaaac
     3481 gctggccacg ctggatgact ggcagagcgc catcgtgccg accctcgaca accgctacgg
     3541 cgacctcgcc ctgcccgcgg cgcccggcgc gccgcccgtg caaacctggc gcttcgcgca
     3601 tcgcatcgag cggcccggcg aagactcgca ggccgccgac ccgacggacg cgggcgactg
     3661 gcagccggtc gaggcgacct tcggccccta tggctggtgg accggccccc ttcccgcgga
     3721 caggctgccc ccgcccctgg cggaccgtgc agacggccaa ccgctcgccc ccacggactg
     3781 gcatcccgcc gtctactcgc ggtcgcgcgg catcgcccgc gatccgatcc actattcgac
     3841 gctggggccg aaggcgcgcg tgccccagga atttctcctg ttcggcccca ccgcgccggg
     3901 cgagggcgtc cgcttccgca ccagcgtggc gtcgcccgcc gaacagcgcg cctacctggc
     3961 gctcggcggc cccggcgcga agcgggcctg gctgaacggc gagccgtgcg gcacggcccc
     4021 cgcgggctac ctctggctgg ccccggtgac tctgcgccag gggcgcaacg tgctggaatg
     4081 ggaactggtg gccgaggcac gagtgaacct gcgcgcctcc tgggcgctgg tgcgcgaccc
     4141 ggcccgctac gcccggcccg agtggctgac cagcgccggt gcgccggtgc gcgactcgcg
     4201 cctgcgcttc tcgaccacgc tggatctccc cttcgcaccc gtcgaaggga cgatccagat
     4261 cggcacggcg gacccctgcc gggtgatcgt gaacggcgtg gaggtgggcc ggcagggcgg
     4321 ctttgacccg tacctggtcg ggcgggtgca gccctacacg acggacgcct ggcggcaagg
     4381 cgcgaatagc gtggtgctcg aagtgctcga ccctggccgc ccagtggcgc tggtcgccga
     4441 tgcgctgatc cacagcgcgg acggcgagac ggccggcctg atgaccgggg cggactggtg
     4501 ggtgcagcgc gacgacggcc cggcgcagcc ggcgaccctg cgccgccggc agtggatcga
     4561 tcagccggcc atcgatgcca gcgagggttt tggcgagatg gacgtggcgt tcgccaacct
     4621 ctggcgccgc ccgcacccgc tacccgatgc cgactggctc gaagacacgc cgcacgacga
     4681 caccgtgctg ccgctggtgc ccgacgcctt cggcagccgc cgggtcgagt ggttccgctg
     4741 gcagattccg cccggcgcca ccgcgatcca cctgccggtg gacggcgagg tgcggctgtg
     4801 ggtggatggc gccgaagtag aggtaagtga ggacacggtc aagcaggccc ggctaggcgg
     4861 cgcgcccgcg gcgtcacgcc aggtggtgat gcgcgtggtg cccgcgagca atctcagcgg
     4921 cggcgccgtg ttcgccggcc cggtcaccta cgagatgggc gtgggccgga tccggcccgg
     4981 cgagtgggcc gctcagggcc tggaagccta cagcggcggc gtgcgctaca gcacggcgtt
     5041 cgcgctcgtt gcgccgccca ccgggcggct gatgctcgac ctggggcggg tgcgcggcac
     5101 cgcggaagtg acggtcaacg ggcaatcctg cggggtgcga gtctggtcgc cctaccgctt
     5161 cgacatcacg gcagccgccc aggtggggca aaacacggtc gaggtgctcg tctgcaacac
     5221 gctcgcaccc tacctgcgcg ccgtcagccc cacgcactac gtgttccccg gcc
//
LOCUS       DASUYC010000516         3927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_718909,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000516 DASUYC010000000
VERSION     DASUYC010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718909"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..1011
                     /locus_tag="VF276_08455"
     CDS             91..1011
                     /locus_tag="VF276_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003393158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEX6606930.1"
                     /translation="MTPADDAARFAQVAQRIAPGGTLLRAWPLTGGVSAQVTALEVAW
                     PGGGTRTVVVRRHGPVDLAHNPHIAAGEFRLLQLVRAAGVPAPAPYFYDESGAIFPTP
                     YLVVEYVEGAPEFDPADVPAFLQQCAATLARIHQVPGVATNLAFLPDHAARLGALLAA
                     PPAQLDDSLDEGRIRAALVAAWPLAPRNAPALLHGDYWPGNLLWRAGRLVAVLDWEDA
                     RTGDPLADLGNSRMEILFALGAEAMAAFTRQYQALTRLDDADLPYWDLAAALCPAGKL
                     AGWDLAPAVEAGMRARHRWFIAQAFAALGG"
     gene            complement(1158..1895)
                     /gene="phoU"
                     /locus_tag="VF276_08460"
     CDS             complement(1158..1895)
                     /gene="phoU"
                     /locus_tag="VF276_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011308776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX6606931.1"
                     /translation="MARETFQRSLGEIQDAVLAMGSMVDRAIERATRSLQTRDLALAR
                     AVISGDPAINAERFRIEEQCLLLIATQAPMASDLRRLAAALNIITDLERMGDHAVGNA
                     RITILIGDEPPLKPLIDVPRMSDICRAMLHASLDAFVRADADAAIAIAYRDDEVDQLY
                     DQVYRELLTFMLADPGTINRATHLLWAAHNLERIADRVTNICERVVFLATGRMAEITG
                     KAARETSGSAAREGAGYRYEGRSARAE"
     gene            complement(2058..2846)
                     /gene="pstB"
                     /locus_tag="VF276_08465"
     CDS             complement(2058..2846)
                     /gene="pstB"
                     /locus_tag="VF276_08465"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEX6606932.1"
                     /translation="MHAETGGPPALAVSDLQAWYGAFQALDNIHLDVAPRQITALIGP
                     SGCGKSTFLRCLNRMHEVVPGARARGQVLFEGEDIYARGVDPVLVRRYIGMVFQKPNP
                     FPTKSIFENVAAGLRIAGAPKSLIAERVERSLRQAALWDEVKDKLNHAATALSGGQQQ
                     RLCLARAIAVEPQIILMDEPASALDPIATLKIEELMLELKQQYTIVIVTHNMQQAARV
                     SDTTGVFMVRPDRAGELIEVGPTREIFTNPRDPRTEAYITGRIG"
     gene            complement(3151..>3927)
                     /gene="pstA"
                     /locus_tag="VF276_08470"
     CDS             complement(3151..>3927)
                     /gene="pstA"
                     /locus_tag="VF276_08470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX6606933.1"
                     /translation="TGAAVIPLVAVLLWVFLQGLPALNVAFFTRLPGPPDDPNQGVAN
                     AVVGTLELLGIAGLISVPAGLGAGIYIAEFGGSRTNTVLRFATDVLSGVPSITIGVFV
                     YALVVLPMQQFSALAGGVALALVMLPLIIRTTEEMLRLVPGSIREAALALGAPTWRTI
                     LRYALPAALPGIVTGGLLALARAAGETAPLLFTALGNNQWNRGLTEPIDALTLKVYFY
                     AGQAYDVWRQISWAGAVVLTLLVLVTSVLARLATRSRYAR"
BASE COUNT          547 a         1352 c         1401 g          627 t
ORIGIN      
        1 gaaggcgcga aggacgcgaa gagtgctttt attgagtttg ttcttcgcgc gctttgcgct
       61 ttcttcgtgc cccattggag tgaggatccg atgacgccgg cagacgacgc ggcgcggttt
      121 gcccaggtgg cgcagcggat cgcgccgggc ggcacgttgc tgcgcgcctg gcccttgacc
      181 ggcggcgtgt cggcgcaggt gacggcgctg gaggtggcgt ggcccggcgg cggtacgcgc
      241 acggtcgtcg tgcggcggca tgggccggtg gacctcgcgc acaacccgca tatcgccgcc
      301 ggcgagttcc ggctgctgca actggtgcgc gccgccgggg tgcccgcgcc cgcgccctac
      361 ttctacgacg agtcgggcgc catcttcccc acgccctacc tcgtggtcga atacgtcgaa
      421 ggtgcgcccg agttcgatcc cgccgatgtg cccgccttcc tgcaacagtg cgccgcgacc
      481 ctcgcccgca tccatcaggt gccgggcgtg gcgacgaacc tggcctttct gcccgaccac
      541 gccgcccgcc tgggcgcgct gctggccgcg ccgccggcgc agctcgacga ctcgctcgac
      601 gaggggcgga tccgcgccgc gctggtcgcc gcctggcccc tggccccgcg caacgccccg
      661 gcgctgctgc acggcgacta ctggcccggc aacctgctct ggcgggccgg gcggctggtc
      721 gccgtgctcg actgggagga cgcgcgcacc ggcgacccgc tggccgacct gggcaacagc
      781 cgcatggaga tcctcttcgc cctcggcgcg gaagcgatgg ccgccttcac gcgccagtac
      841 caggccctga cccgcctcga cgacgccgac ctaccgtact gggacctggc cgccgcgctg
      901 tgccccgcag gcaaactggc cgggtgggat ctggcccccg cggtcgaagc cggcatgcgc
      961 gcccggcacc gctggttcat cgcgcaggcg tttgccgcgc tgggcgggta gcgcggaggc
     1021 tcaagcctcc ggctaccctt acgaaggccg cctgcgcggc ctgtcactcc caccccagcc
     1081 cgcgcaggcg ggctttgtat acgtagcccg cccgttgacg ggccgggcgg ggccgctacc
     1141 gcgcggtcga ccacaattca ctcagcccgc gctgagcggc cttcgtaacg gtagcccgcc
     1201 ccttcacggg ccgcgctacc gctcgtctcg cgggcggctt tgcccgtgat ctcggccatg
     1261 cggccggtgg cgaggaagac gacgcgctcg cagatgttgg tcacgcggtc tgcgatgcgc
     1321 tccaggttgt gcgcggccca gagcaggtgc gtggcgcggt tgatcgtgcc gggatcggcc
     1381 agcatgaacg tcagcagttc gcggtagacc tggtcgtaaa gctggtccac ttcgtcgtcg
     1441 cggtaggcga tggcgatggc cgcgtcggcg tcggcgcgca cgaaggcgtc cagagacgcg
     1501 tgcagcatcg cgcggcagat atccgacatg cgtggcacgt cgatcagcgg cttcagtggc
     1561 ggctcgtcgc cgatcaggat ggtgatgcgg gcgttgccca ccgcgtggtc gcccatgcgc
     1621 tccaggtcgg tgatgatgtt gagcgctgcg gccagccgcc gcaggtcgct cgccatgggc
     1681 gcctgtgtgg cgatgagcag caggcattgc tcctcgatgc ggaagcgctc ggcgttgatg
     1741 gccgggtcgc cgctgatgac ggcgcgggcc agggccaggt cgcgcgtctg cagcgaccgg
     1801 gtcgcgcgct cgatggcccg gtccaccatg ctgcccatcg ccaggacagc gtcctggatc
     1861 tcccctaaac tccgttggaa tgtctcgcgg gccattggtt gtggttcctc tctaatatgc
     1921 ggcgggccgg tttcaaaccg gcccctacaa cgcctaccgg gtgacaggcc ggtttgaaac
     1981 cggcccccac accgcctacg gggtggcggg ccggggtgca accggcccct accgcgcgaa
     2041 tgcacgatgg ccgggggcta gccaatgcgg ccggtgatgt aggcttcggt gcgcgggtcg
     2101 cgcgggttgg tgaaaatctc gcgcgtcggg ccgacctcga tcagttcgcc cgcgcgatcc
     2161 gggcggacca tgaacacgcc cgtggtgtcc gatacgcgcg ccgcctgctg catattgtgg
     2221 gtcacgatga cgatggtgta ttgctgcttg agttcgagca tcagttcttc gatcttcagc
     2281 gtggcgatgg ggtcgagcgc gctggccggc tcgtccatca ggatgatctg cggctcgacg
     2341 gcgatggcgc gggccaggca gaggcgctgt tgctgcccgc ccgatagcgc cgtggcggcg
     2401 tggttgagct tgtccttcac ctcgtcccag agtgccgcct ggcgcaaact gcgctcgact
     2461 cgctcggcga taaggctctt cggcgccccg gcgatacgca gccccgccgc cacgttctcg
     2521 aagatcgact tggtggggaa ggggttgggc ttctggaaga ccatgccgat gtagcggcgc
     2581 accagcaccg ggtccacgcc ccgcgcgtag atgtcctcgc cctcgaacag cacctgcccg
     2641 cgcgcccgcg cccccggcac gacctcgtgc atgcggttga ggcagcgcaa gaaggtcgac
     2701 ttgccgcagc ccgacggacc gatcagcgcg gtgatctgcc gcggcgccac gtcgaggtgg
     2761 atgttgtcga gcgcctggaa cgcgccgtac caggcctgca ggtcgctgac cgccagcgcg
     2821 ggcggcccgc ctgtctccgc gtgcacgtgc gtatcctccg gcaccggcaa atactgggga
     2881 ggcagggcca ggtccgcccg ggcgccgtgg ccgttcgcgc gccccccagg atgcgctgta
     2941 ggggccggtt tcaaaccggc ctgctgcccc gtgtgcgttg tgggggccgg tttcaaaccg
     3001 gcctgctgct ccgtgtgcgt tgtgggggcc ggtttcaaac cggcctgttc gtatatggga
     3061 tcctcgccgg attgcgttgt aggggccggt ttcaaaccgg cctgctgccc attcccctca
     3121 ctgtggagga cgacgactaa cgattgcgta ttatcgagca tagcggctcc ttgtggcgag
     3181 gcgggccagg acgctggtga cgagtaccag cagcgtcaag accacggcgc ccgcccatga
     3241 aatctggcgc cagacgtcat acgcctggcc cgcgtagaaa tagaccttca gggtcagtgc
     3301 gtcaatcggt tcggtcagcc cgcggttcca ctggttgttg cccagcgcgg tgaacagcag
     3361 cggcgcggtt tccccggcgg cgcgggccag cgccagcagg cccccggtga cgatgccggg
     3421 cagggcggcg ggcagcgcat agcgcaggat ggtgcgccag gtgggcgcgc ccagggccag
     3481 cgccgcctcg cgaatcgagc cgggcaccag ccgcagcatc tcctccgtgg tgcggatgat
     3541 cagcggcagc atgaccagcg cgagggccac gcccccggcc agcgccgaga actgctgcat
     3601 cggcagcacg acgagcgcgt agacaaacac gccgatggtg atcgagggca cgccgctcag
     3661 cacgtcggtg gcgaagcgca ggaccgtgtt ggtgcggctg ccgccgaact cggcgatgta
     3721 gatgcccgcg cccaggccgg cgggcacact gatgagcccg gcgatgccca gcaattcgag
     3781 cgtgcccacg acggcattgg cgacaccctg gttggggtcg tcgggcgggc cgggcagccg
     3841 ggtgaagaag gcgacgttga gcgcgggcag cccctgcagg aacacccaga gcagcacggc
     3901 caccagcggg atcacggcgg cccccgt
//
LOCUS       DASUYC010000517         6164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_287353,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000517 DASUYC010000000
VERSION     DASUYC010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6164
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287353"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..500)
                     /gene="lepB"
                     /locus_tag="VF276_08475"
                     /pseudo
     CDS             complement(1..500)
                     /gene="lepB"
                     /locus_tag="VF276_08475"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
     gene            781..1455
                     /locus_tag="VF276_08480"
     CDS             781..1455
                     /locus_tag="VF276_08480"
                     /inference="COORDINATES: protein motif:HMM:NF018822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LppX_LprAFG lipoprotein"
                     /protein_id="HEX6606934.1"
                     /translation="MLLCLAVVACGDSPVPPAGDIITKSVAAMRDITSLHFLLDTNKL
                     ETYPKGLFLVHAEGDAAKPDKLRATAKALLLGTAIEVQVVGLGTEQYMTDPASGRWQR
                     MPPSFNVLAAFDPSKGIADILANAKDPQNDGTETVDGVACYRVKTTLSPDALKALSTE
                     VNATAPLPARLWISTADSRLVQVELSGPLMNGEPSNIVRTVKFSQFNAPVDIQKPTVG
                     SAPSKP"
     gene            1452..3065
                     /locus_tag="VF276_08485"
     CDS             1452..3065
                     /locus_tag="VF276_08485"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6606935.1"
                     /translation="MSTPPALAEAPSPTPARSGPGPRAGLAIVASGVVLAALDQTVVV
                     TVLYKVQEEYQIAPIHLDQLGWIVTAYLLGYTVTLPLMGRVADVFGRRRIYLLSLLVF
                     LIGSVLAALSQTLPWLVAARVIQAVGGGALLPVSMAMVRGWYGEGRRAFALGLLGAAA
                     EGGGVLGPLWGVAIISVMDWRWIFWLNLPLGAVIGALVLWTAPHPRYPGRIDWLGAGL
                     LGGALLALTLGLSSSSSNSAIAGIGGFDTSAEASSPIWQTPALLIGAVVLTAAFLWWE
                     RRAPVPLLPAGLFRRVPFTLAHVLNAMVGMALITAMVNIPLLVASVLSGGATAGGLLL
                     LHLTLFIPVGAVIGGALAERLSYRVVAATGMVCTAIGFWLMSAWHTDTADLQMTLNLA
                     LAGLGFGLVIAPITSTTLAWVDEEQAGLASALVNTARMVGMMVGLSALSAWGLELFKS
                     LMANHPAPLPGLGETEAAYNARLAEYQQILANASLQVYTLGFLVAAVVCALAILPALG
                     LRRRANLSPSPSPSRGGESRSSFPPREGG"
     gene            3247..4998
                     /locus_tag="VF276_08490"
     CDS             3247..4998
                     /locus_tag="VF276_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606936.1"
                     /translation="MRVTSRLLPVIALLLSALLLLGKPPARAAQPEPSAWTLIDLHLP
                     QLNMLTSVFMVDGPDGAWVTTNGSAVLRLARRNGTWTVQERAEFHGFALNAVVAPSET
                     NVWAVGDEGMIVHKDATGWHTVPNPVWGADLYTIQMFGSGEEGWAAGSLPPDFSSDFK
                     RHSVLLHYQHGTWSRDPAISDEGVTINSLHFGAGGGWAVGQVMVGESAAPVAWRYTGD
                     TWQRENFPACTFCAPPLLAVRSLGRSEAWAVGLRRAGFMGIEPSVPVAFQRTVNGWRD
                     VLAGQPVVGDPDPNRYAAGLDALSFSPDGFGLAVGHQQHEDRGAVLPYIVSYRPDGRW
                     HYEAVPAFSPGYLTGVSQADSTHALAVGVEGLVLAYGYPAAPTPQPIATPTTARPTDR
                     VADPRQPGVVYYPQTGHTLRGRFQVYWTGHGGLPQFGYPLTEEFAEVSPTDGRTYTVQ
                     YFERARFEYHPEYGPEADVLLGLLGRTITAGRQDEPAFRPQPGPAPAGRWFPETAHTL
                     APEFLAYWTSHGGLPVYGYPISEAVLETSPTDGKAYRVQYFERNRLEYHPELPEPYRV
                     SLGLLGVQVLQMRGWLP"
     gene            complement(5043..5651)
                     /locus_tag="VF276_08495"
     CDS             complement(5043..5651)
                     /locus_tag="VF276_08495"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX6606937.1"
                     /translation="MADQTLNVGILIFDDVEVLDFCGPFEVFASAGQATGATGDGAPF
                     HVFTVAERAEVVHCRGNLLVQPHYIIADHPRIDLLVVPGGWGTRREVHNPVLLDWIAA
                     QDRWTTLTLSVCTGAFLLAERGILDGHAATTHWASIDRLRQGYPQIDVRDDRRYVDAG
                     HVVTSAGVSAGIDMALYVVSRLCGAEVARATARRMEYPWTPD"
     gene            complement(5783..>6164)
                     /locus_tag="VF276_08500"
     CDS             complement(5783..>6164)
                     /locus_tag="VF276_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX6606938.1"
                     /translation="LLPRPESGAPAAVPDLPPADLVVDCTGHPTGQQTALRLLRPRGT
                     LVLKSTFHGDATWNPTDFVVNEWTLVGSRCGPFAPALRLLSRRLIDTAPLIEATYPLS
                     EGVAALEHAARLGALKVLLRPDRS"
BASE COUNT          951 a         2119 c         2098 g          996 t
ORIGIN      
        1 ggctcgtcga gtttgtggcc gttcacgtag acgccgtcgt aggccttgac ctgcaccgcc
       61 tcgcccggca cggcgatcac gcgcttgatc tagtcctcgg attcgccctg caccgggggc
      121 cggaacacca cgatgtcgcc gcgcaggggg ccgcccagcg ggtaggtgga ccccaggtgg
      181 aagtaggacg cgcggtcgat aaacaggcgc tcctggtcgg ccagcgtggg atccatgctg
      241 tggccgagca cctggcggtt ctggacgacg ctctgcacca gcagaaagat catcaggctc
      301 agcacgaccg cttcgatgac ttcgcggacg atcgacttgc tgccataatg ccgctcggca
      361 acggtgatat cggcatcggt aacgggcagg agatagcggg gggtttccgc tggggcgggc
      421 acgaccgtgt ccgcggggcg cgcttcgtgt gcggattgat ccaagttgac agacctcggc
      481 cctgagggcg aacgggccac gcgcgcaggt cacggcgcag ccggctggta gatacgtggg
      541 tgccgggttc ccggccagac tcgcccctgg cgagtcaaat actacccata gattatacgc
      601 gcaaacgggc caaaagttac accctcgtcc caggttgaca tgaacgcgcc ctatcggcta
      661 ggataggggc agaccggcac tccccgccgc gcggcggcca tgggacccgg cataggacgg
      721 agcacgaccc ttatgcgtca ctcttttgta acgggccggg cagcccgttg gattgcgttc
      781 ttgctgctat gcctggccgt ggtcgcctgt ggcgactcgc cggtgccgcc cgccggcgac
      841 atcatcacca agagcgtcgc ggccatgcgc gacatcacca gcctgcactt cctgctcgac
      901 accaacaaac tggagaccta ccccaagggc ctcttcctgg tccacgccga aggcgatgcg
      961 gccaagcccg acaaactgcg cgccacggcc aaagccctgc tgctgggcac cgccatcgag
     1021 gtgcaggtcg tcggcctggg caccgagcag tacatgaccg acccggccag cgggcgctgg
     1081 cagcgcatgc cgccgtcgtt caacgtgctg gccgccttcg atcccagcaa aggcatcgcc
     1141 gacatcctcg ccaacgccaa ggatccgcaa aacgacggca ccgaaaccgt ggacggcgtc
     1201 gcctgctacc gcgtgaagac taccctgagc cccgatgccc tcaaggcgct cagcaccgaa
     1261 gtcaacgcca ccgcgccgct gcccgcccgg ctctggatca gcaccgccga ctcgcgcctg
     1321 gtccaggttg agctgtcggg cccgctgatg aacggcgaac cgagcaatat cgtccgcacg
     1381 gtcaagttct cgcagttcaa cgcacccgtc gatatccaga aaccgaccgt cggctccgcg
     1441 ccgagcaagc cgtgagcacg ccgcccgccc tggccgaggc gccatcccct acccccgcgc
     1501 gtagcgggcc ggggccgcgc gccgggctgg caattgtcgc gtcgggcgtc gtcctggccg
     1561 cgctcgacca gacggtcgtc gtcacggtgc tctataaggt ccaggaagag taccagatcg
     1621 cgccgatcca tctcgaccaa ctcggctgga tcgtgacggc gtacctgctg ggctacaccg
     1681 tgaccctgcc gctgatgggc cgcgtggccg atgtcttcgg gcggcggcgc atctacctgc
     1741 tgagcctgct cgtcttcctg atcggctcgg tgctggccgc gctctcgcag acgctgccgt
     1801 ggctggtggc cgcgcgggtg atccaggcgg tgggcggcgg ggcgctgctg ccggtcagca
     1861 tggcgatggt gcgcggctgg tacggtgagg ggcgccgcgc attcgcgctg ggcctgctgg
     1921 gcgcggcggc ggaaggcggc ggcgtgctcg gcccgctctg gggtgtggcg atcatcagcg
     1981 tcatggattg gcgctggatc ttctggctga acctgcccct gggcgccgtc atcggcgcgc
     2041 tcgtgttatg gaccgcgccc catccccgct atccgggccg gatcgattgg ttgggggccg
     2101 ggctgctggg cggcgcgctg ctggcgctga cgctggggct ctccagttcg tcctccaaca
     2161 gcgccatcgc cggcatcggc ggcttcgaca cgagcgccga ggcgagcagc cccatctggc
     2221 agacgccggc cctgctcatc ggcgcggtcg tgctgacggc ggccttcctc tggtgggaac
     2281 ggcgcgcgcc ggtgccgttg ctgccggcgg gcctgttccg ccgtgtgccc ttcaccctgg
     2341 cccatgtgct gaacgccatg gtcggcatgg cgctgatcac ggccatggtg aacatcccgc
     2401 tgctggtcgc cagcgtgctc agcggcgggg ccaccgccgg cgggctgctg ctgctgcacc
     2461 tgaccctgtt catccccgtg ggcgccgtga tcggcggcgc gctggccgag cggctgagct
     2521 accgcgtggt ggccgccacc gggatggtct gcacggcgat aggtttctgg ctgatgagcg
     2581 cctggcacac cgacacggcc gatctccaga tgacgctgaa cctggccctg gccgggctgg
     2641 gcttcggcct ggtcatcgcc ccgatcacca gcacgaccct ggcctgggtg gacgaagagc
     2701 aggccgggct ggcctccgcc ctggtcaata cggcgcgcat ggtcggcatg atggtcggcc
     2761 tctcggcctt gagcgcctgg ggcctggaac tgttcaagag cctgatggcg aaccaccccg
     2821 ccccgctgcc gggcctgggc gagaccgagg ccgcgtacaa cgcccgcctg gccgagtacc
     2881 agcagatcct ggcgaacgcc agcctgcagg tctacacgct gggcttcctg gtcgccgccg
     2941 tcgtctgcgc gctggcgatc ctgcccgcgc tgggcctgcg ccgccgggcc aacctctccc
     3001 ccagcccctc cccgtcgcgg ggaggggagt cgcgttcttc cttcccgccg agggaaggcg
     3061 ggtagggcgg ttcggttcgg ggacgcaaca gtcgttcatc ggcgaacacc gccgagacct
     3121 ctctcgcagc caccacctcg ccacccaggc aactccccgc gcagtacaag gcaaccacac
     3181 agcggatggt ccgttgatac ggatgagagg cacattacgc aacaataccc cgcctggagg
     3241 ccctctatgc gtgtcacatc ccggttgctg cctgttatcg ccctgctgct gagtgctctg
     3301 ttgctcctgg gcaagccgcc ggcccgcgcc gcgcagcccg aaccctcggc ctggaccctg
     3361 atcgatctcc acttgccgca gttgaatatg cttaccagcg tgttcatggt cgatggcccg
     3421 gacggcgcct gggtgacaac caacggcagc gccgtgttgc gcctggcccg tcgcaatggc
     3481 acctggaccg tgcaggagcg ggccgagttc cacggcttcg cgctgaacgc tgtggtggcg
     3541 cccagcgaaa ccaacgtctg ggccgtgggc gacgaaggca tgatcgtgca taaagatgcg
     3601 accggctggc acaccgtccc caatccggtt tggggtgcgg atctctacac gatccaaatg
     3661 ttcggcagcg gcgaggaagg gtgggccgcc gggagcctgc cgcccgactt cagctccgat
     3721 ttcaagcgcc actccgtgtt gctgcactac cagcacggca cgtggtcgcg cgacccggcg
     3781 attagcgacg agggggtcac gattaacagc ctccattttg gggccggtgg cggttgggcc
     3841 gtgggccagg tcatggtggg ggaaagcgcc gccccggtgg cgtggcgcta taccggcgac
     3901 acctggcagc gggaaaactt cccggcttgc accttttgcg cgccgcccct gctggcggtg
     3961 cggagcctcg gccgatcaga agcctgggcg gttgggctac ggcgggccgg gttcatgggc
     4021 attgaaccct cggtgccggt cgccttccag cgtaccgtca acggatggcg cgacgtattg
     4081 gccggccagc ctgtagtggg cgaccccgac ccgaaccgct acgcggcggg cttggacgcg
     4141 ctgagtttta gcccggacgg atttgggctg gcggtggggc accagcagca cgaggatcgt
     4201 ggcgccgtgc tgccctatat cgtgagctac cggcccgatg gccgctggca ctacgaagcg
     4261 gtccccgcgt tcagccctgg ttacctgacg ggggtcagcc aggccgacag cacccatgcc
     4321 ctggcggtgg gcgtggaagg gctggtgctg gcttatggct atccggcggc ccccacgccc
     4381 caacctatcg ccacgccgac aacagcacgc cccaccgacc gcgtggcgga ccccaggcag
     4441 ccgggcgtcg tctactaccc gcaaactggg catacattgc gtggccgctt ccaggtctac
     4501 tggaccgggc acggtgggct gccgcagttc ggctatccgc tgaccgaaga gttcgccgag
     4561 gtcagcccga ccgacggtcg aacttacaca gtgcagtatt tcgaacgcgc ccgcttcgag
     4621 tatcacccgg agtatgggcc ggaggcggac gtgctgctgg ggctactggg gcggacgatc
     4681 accgcgggcc gccaggatga gccggccttc cggccccaac ccggtcccgc gcccgccggt
     4741 cgatggttcc cggaaacggc ccatacgctg gcgcctgagt tcctggccta ctggaccagc
     4801 cacggcgggc tgcctgtcta cggctatccg atcagcgaag cagtgctgga aaccagcccc
     4861 acggacggga aggcttatcg cgtgcaatac ttcgagcgca accgcctgga gtaccacccc
     4921 gagctaccgg agccgtatcg cgtgagcctg ggcttgcttg gggtgcaggt gttgcagatg
     4981 cgcggctggc tgccataagg ccggcaggac ttcccggttc gtgctaaccg tcgggctagc
     5041 cactaatccg gcgtccaggg gtactccata cgccgggccg tggcgcgggc cacctctgcg
     5101 ccgcacaggc ggctgacgac gtagagcgcc atgtcgatgc ccgccgagac ccccgccgac
     5161 gtgaccacat ggcctgcatc gacgtagcgc cggtcgtcgc gcacgtcgat ctgtgggtag
     5221 ccctggcgca ggcggtcgat gctggcccag tgggtggtgg cggcatggcc gtcgaggatg
     5281 ccccgttcgg ccagcaggaa ggcgccggtg cagaccgaca gggtcagcgt ggtccagcga
     5341 tcctgcgcgg cgatccagtc gagcagcacc gggttgtgga cttcgcggcg cgtgccccac
     5401 ccgcccggca ccaccagcag gtcgatccgc ggatggtcag cgatgatgta atggggttgc
     5461 accagcaggt tgccccggca atgcacgacc tccgccctct cggccacggt aaacacgtgg
     5521 aatggcgcgc cgtcgccggt cgccccggtg gcctgcccgg cgctggcgaa gacctcgaag
     5581 gggccgcaga agtccagcac ctccacgtcg tcgaagatga gaatgcctac gttcagcgtc
     5641 tgatccgcca tgagaatcgg ttcctttcac tgcaaacggc cataacggta gcggtgtagg
     5701 ggccggtttg aaaccggccc gcgccccgat gtcaccctat cgttgtaggg gccggtttga
     5761 aaccggcccg cgccctgccg ccctatgagc ggtcgggccg cagcagcacc ttgagcgcgc
     5821 cgagccgcgc cgcgtgttcc agcgccgcca cgccttcgct taagggatat gtggcctcga
     5881 tcagcggcgc cgtgtcgatc aggcgccggc tcaacaggcg caaagcgggc gcgaaggggc
     5941 cgcagcgcga acccaccagg gtccactcgt tgactacgaa gtcggtgggg ttccaggtgg
     6001 cgtcgccgtg gaacgtgctc ttgagcacga gcgtaccacg gggccgcagc aagcgcaagg
     6061 ccgtttgctg tccggtcggg tgcccggtgc aatcgactac gagatcagcg ggcggcagat
     6121 caggcacggc ggcgggcgca cccgactcag ggcgcggcag caac
//
LOCUS       DASUYC010000518         4551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1951740, whole genome shotgun sequence.
ACCESSION   DASUYC010000518 DASUYC010000000
VERSION     DASUYC010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4551
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1951740"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1092
                     /locus_tag="VF276_08505"
     CDS             <1..1092
                     /locus_tag="VF276_08505"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX6606939.1"
                     /translation="GRLRAAAATYRELDPIIANPTGLQGPRGLYSSPAYYVGLGDLHR
                     EWNELDAADGYLAQALELLPHTLAVDAGYVARGYIAWARLQAARGEPGAAQQTLAAFA
                     DLAHGRGLAPHLIARGAAVQAQLALAAGNLAAAVTWADASGLHVDDPIAFPREAEYLV
                     LARTWIGRAGRDAAGDYLAQALHLLDRLLADASAKARGASVLEILIVQAQALWAQGAR
                     PAALATLGQALALAAPEGYVRRFVDEGPALRVMLEAAYAGGIEPDYVTRLLAACAPRP
                     QDTRERAALAPVLAPPAASRAALAEPLSERERQVLRLIVDGQSNAEIARALVIAVSTV
                     KTHTNSIFGKLGVTSRTQAIARARALHLL"
     gene            1412..1642
                     /locus_tag="VF276_08510"
     CDS             1412..1642
                     /locus_tag="VF276_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606940.1"
                     /translation="MSLHYQITVQEYLEESWSDWFDSLRITHASDGTTTLAGAVRDQT
                     ALYGLLAKLRDLGLTLLSVTPATGPPATEAVP"
     gene            1698..2720
                     /locus_tag="VF276_08515"
     CDS             1698..2720
                     /locus_tag="VF276_08515"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6606941.1"
                     /translation="MATLTNTLHVPPTATSMARRILRGIARGLGVVLALVAGLALAGA
                     AYEAIAARSDAQTYPPPGQMVDVGGYRLHIYCVGSGSPTVVLDAGLSRMSLDWTLVQP
                     AIGQTTRVCAYDRAGMGWSDSGPGPRTPAQIAGELHTLLGNAGIAGPYVLVGHSLAGK
                     NIRLFAQQHPDEVVGMVLVDARGEYVDAHTSPAEAQGFQQALAQQATIYGLARAVGLV
                     RLIGASQEGVPEVPNETRQAMALFSTGQRGLATTAAEGQQRAADDGQLQAAPGLGALP
                     LIVLASGQNMAQLPYWADAQRQQAALSTNGRLIVAEGSGHGINLERPALVIDAVRQVV
                     AQARGH"
     gene            2794..3549
                     /locus_tag="VF276_08520"
     CDS             2794..3549
                     /locus_tag="VF276_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6606942.1"
                     /translation="MALATWWRGDPLPALDPLPDFHVAAPRDAALIAQVGGLTAAQAQ
                     RRLDQGHQPYLAYIGAEPAGYGWLAQQAAEIGELDLRFALPPADRYLWDFATLPAWQG
                     YGIYPRLLQHILRQQALPVERVWIIYAPENTPSGAGMHKAGLLPAGQLSFDSDGAVGL
                     AVTGAPHRARAGAALLGVPIIETVPAPCWACGGATATHVPPGDSAGCWPPLRPTTTPC
                     TCAVPLKPSKTPAGAHQHHKHEDVTAEQGETVA"
     gene            3546..>4551
                     /locus_tag="VF276_08525"
     CDS             3546..>4551
                     /locus_tag="VF276_08525"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX6606943.1"
                     /translation="MKYFRIVVRIGIYTLAFALPIVALAALGLYSTLQLTGPAPAAPA
                     APAITPPRARSFDPAKPTVAIVLGSDRTESTDFLIPYELFSATGAYNVYAVAPARQMT
                     TLAGGLEVMPDFSYAELETLLGRAPAIVMMPAFPDVTSPQNQPVLAWIKQQAARGSFM
                     FSICVGAEAFAATGLLDGRTATTHWGDIDRIEQAYPAVRWVRGVRYVDGGDYMTSAGI
                     TSGIDAVLHYIAQHNGDELARAVAQQLHYPSYAFVDAPQVQQESAGAGLMLVGLSNIV
                     FHPLHPAAGVLLYDGIGELEVAATYDTYAASYTTRLRSVAQTRQVITTRHGLQLVPRW
                     S"
BASE COUNT          684 a         1614 c         1601 g          652 t
ORIGIN      
        1 ggtcggctgc gagcggcggc ggccacctat cgggagctgg acccgatcat tgccaacccc
       61 accggtctcc agggaccccg cgggctatac agcagtcccg cctactatgt cggcctcggc
      121 gatctgcatc gcgagtggaa tgagttggac gccgcggacg gctacctggc gcaggcgctg
      181 gagttgctgc cgcacacgct ggcggtggat gccggctatg tggcccgcgg ctatatcgcc
      241 tgggcgcgcc tgcaggccgc gcggggggag cccggcgccg cgcagcagac gctggcggcc
      301 ttcgcggacc tggcgcacgg gcggggcctg gccccccacc tgatcgcgcg cggggcggcc
      361 gtgcaggccc agctcgcgct cgccgccggc aatctcgccg ccgccgtcac ctgggcggac
      421 gcgagcgggt tgcacgtgga tgatcccatc gcgttcccgc gcgaagccga gtacctggtg
      481 ctggcccgca cctggatcgg gcgtgcgggc cgcgacgccg ccggcgatta cctcgcgcag
      541 gcgctgcacc tgctcgaccg cctgctggcc gatgcctccg ccaaggcccg cggggccagc
      601 gtgctcgaaa tcctgatcgt gcaggctcag gcgctgtggg cacaaggcgc gcgccccgcc
      661 gcgctggcga cgctggggca ggcgctggcc ctggccgcgc ccgaaggcta cgtccggcgc
      721 tttgtggacg aggggccggc gctgcgggtc atgctcgaag ccgcctatgc cggcggcatt
      781 gagccggact acgtgacccg gctgctggcg gcctgcgccc cgcgcccgca ggacaccagg
      841 gagcgggccg cgctggcgcc ggtgctcgca ccacccgccg catcccgcgc ggcgctcgcc
      901 gaacccctca gcgagcgcga gcgccaggtg ctgcgcctga tcgttgacgg ccagtcgaac
      961 gcggagatcg cgcgggcgct ggtgatcgcc gtcagcacgg tcaagaccca caccaacagc
     1021 atcttcggca agctgggcgt caccagccgc acccaggcga ttgcgcgggc ccgcgcgctg
     1081 cacctgcttt agtagccggc ccctacaccg ggcggcaggc cggtttgaaa ccggccccta
     1141 cagcgcaatc cgggcggcag gccggtttgt agccggcccc tacaccgggc ggcgggccgg
     1201 tttgaaaccg gcccctacag cgcaatccgg gcggcgggcc ggattgaaac cggcccctac
     1261 accgcgaaat tagcgtggct gactactagc ccaagcctcc ccaaccgcaa cctggaatcc
     1321 acccgccgat ccacctttgg gtgggtgtgc atccacccta gcctcggcta tgctgaacct
     1381 gttgatgccg caggatcgaa cggagccgat gatgagccta cactaccaga tcacggtgca
     1441 agaatatctc gaagagagct ggtccgactg gtttgacagc ctgcggatca cccacgcgag
     1501 cgacggcacc acgaccctgg cgggcgcggt tcgcgatcag accgcgctct acggcctcct
     1561 ggccaagctg cgggacctgg ggctcacgct gctctcggtc acgcccgcca ctgggccgcc
     1621 ggccaccgag gccgtgccgt gagtgtgttc atcagcgacc cggacacatg gaacaggaac
     1681 ggatggaggt actcaccatg gcaactttaa ccaacacact gcatgtcccg cccacggcca
     1741 cgagcatggc ccggcggatc ttacgcggga tcgcccgcgg gctgggcgtg gtgctggcgc
     1801 tggtcgccgg cctggcgctg gccggggccg cctatgaagc catcgccgcg cgcagcgatg
     1861 cgcagaccta tccgccgccg ggccagatgg tcgacgtggg cggctatcgc ctgcatatct
     1921 actgcgtggg cagcgggagc ccgaccgtgg tgctcgacgc cgggctgagc cgcatgtcgc
     1981 tcgactggac cctggtgcag ccggcgatcg gccagacgac gcgggtctgt gcgtatgacc
     2041 gcgccggcat gggctggagc gacagcgggc cagggccgcg caccccggcc cagattgccg
     2101 gcgaactgca cacgctgctg ggcaacgcgg gcatcgccgg gccctatgtc ctggtcgggc
     2161 actcgctggc cggcaagaac atacgcctgt ttgcgcagca gcaccctgac gaggtcgtgg
     2221 ggatggtgct ggtggacgcg cgcggcgaat atgtggacgc gcacacctcg ccggcggagg
     2281 cgcaaggctt ccagcaggcg ctggcgcagc aggcgacgat ctacgggctg gctcgcgccg
     2341 tgggcctggt ccggctcatc ggcgcgagcc aggagggggt gccggaggtg ccgaacgaga
     2401 cgcgccaggc gatggcgctg ttcagcacgg ggcagcgggg gctggccacc accgccgccg
     2461 aagggcagca acgcgccgcc gacgacggcc aactccaggc ggcgccgggg ctgggcgcgc
     2521 tgccgctgat cgtcctggcg tccggccaga atatggcgca gcttccctac tgggccgacg
     2581 cccagcggca gcaagcggcg ctgagcacga acgggcgcct gatcgtggct gaaggcagcg
     2641 ggcacggtat taacctggag cggcccgccc tggtcatcga cgcggtgcgg caggtggtcg
     2701 cgcaggcgcg gggccactaa gccccgcgca ctgccggcgg gtttgcgccg ggtcgcgcgc
     2761 ggcccggcga cggtactaac aggaggcgat atcatggcgc tggcaacctg gtggcgcggc
     2821 gatcccttgc ccgccctcga ccctctcccc gactttcacg tcgcagcacc gcgcgacgcg
     2881 gcgctgatcg cccaggtggg cggattgacc gcggcccagg cgcagcggcg gctcgaccag
     2941 gggcaccagc cctacctggc gtacatcggg gccgaaccgg ccggctatgg ctggctggcg
     3001 cagcaggcag ccgagattgg cgaactcgac ctgcgctttg ccctgccgcc cgcggaccgc
     3061 tacctgtggg actttgccac gctgcccgcc tggcaagggt acggcatcta cccgcggctg
     3121 ttgcagcaca ttctgcggca gcaggcgctg ccggtcgaac gggtgtggat catctacgcg
     3181 ccggagaaca cgccgtcggg cgccgggatg cacaaggccg ggctgctgcc cgcgggccaa
     3241 ctgtcgtttg atagcgacgg cgccgtcggg ttagccgtga ccggcgcgcc gcaccgcgcg
     3301 cgggccgggg cggccctgct gggggtgccg atcatcgaga ccgtgccggc gccctgctgg
     3361 gcgtgcggcg gcgcgaccgc cacgcacgtc ccgccagggg acagcgcggg ctgctggccc
     3421 ccgctccgtc cgacgacgac accttgcacg tgcgccgtgc cgctcaagcc cagcaagacc
     3481 cccgccgggg cgcaccaaca tcacaagcat gaagacgtta ctgctgaaca aggagaaacc
     3541 gtagcgtgaa gtactttaga atcgtcgttc gcatcgggat atacacgctg gcgttcgcgc
     3601 tgccgattgt ggcgctggcc gcgctgggct tgtattcgac gctgcaactg accgggcccg
     3661 cccctgctgc acccgccgcg ccggccatta cccctccgcg tgcccggagc tttgacccgg
     3721 ccaaaccgac ggtcgccatc gtcctgggca gcgaccggac cgagagcacc gattttctca
     3781 ttccctacga gctcttctcg gccaccggcg cctacaacgt gtatgccgtc gcgcccgcgc
     3841 ggcagatgac cacgctggcg ggcggcttag aggtgatgcc cgacttctcg tatgccgaac
     3901 tcgaaaccct gctgggccgc gcgcccgcca tcgtcatgat gccggccttc cccgacgtga
     3961 cgagcccgca aaaccagccg gtgctggcct ggatcaagca gcaagcggcg cgggggtcgt
     4021 tcatgttctc catctgcgtg ggggccgaag cgtttgccgc gacgggcctg ctggacggcc
     4081 ggacggcgac gacgcactgg ggcgatatcg accgcatcga acaggcctat ccggcggtgc
     4141 ggtgggtgcg cggcgtgcgc tatgtggacg gcggcgacta catgacctcc gccgggatca
     4201 cgtcgggcat tgacgccgtt ctgcactaca tcgcccagca taacggcgac gaactggcgc
     4261 gggccgtcgc gcagcaactg cattatccga gctacgcctt tgtggatgcg ccgcaggtgc
     4321 aacaggaatc cgcgggcgcc ggcctgatgc tggtggggct gagcaacatc gtgtttcacc
     4381 cgctgcaccc ggcggccggc gtgttgttgt acgacggcat cggcgagctg gaagtggccg
     4441 ccacctacga cacctatgcg gcgtcgtaca ccacccggtt gcggagcgtg gcccaaaccc
     4501 gccaggtcat taccacccga cacgggttgc aactggtccc gcgctggagc t
//
LOCUS       DASUYC010000519         3701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1400912, whole genome shotgun sequence.
ACCESSION   DASUYC010000519 DASUYC010000000
VERSION     DASUYC010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1400912"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1437
                     /locus_tag="VF276_08530"
     CDS             <1..1437
                     /locus_tag="VF276_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606944.1"
                     /translation="PLARFLPVLAWMAVLGGAVLLGTRLLPVAARGVRAGVGRALVLV
                     LLVLSSYGLQVGLLGLKDANPQRLLVERVTNRVFTSYFTLAAAPTTHLSTFFRDYPAV
                     FNTKLCPHCHEHPPGPALFYWVILRGIASLPESLQHDLGAAARRLVGDSPALAALQPP
                     LTDAQLVGAVAGGNLVLLLAATLVLPLYGLARLLAPRAPPWWLAGLAVAVPGVLLMAP
                     EFDQGLGLVTAGALYCGLRGLRTSQPGRAVAWAATAGLILAVGLFFTWAPVVLFGAFV
                     GLGFLAWGYGRSALFAPADTGGRLPARQVVAWLAALVAATALVELVLTVGLGFDLPYV
                     LQYNLNNAAFAEARRPYAVWIFFGPLDFLQFLGLPLAIAALAALILPARAAAAPAPGA
                     PLARVAPWLGRINPYAALFWGLLLAIDLAGRTKAEQGRLLLYLAPLALVAVYVWVERT
                     RPAPRILAAVLAAQLLVTLVLGARWFVP"
     gene            complement(1474..3105)
                     /locus_tag="VF276_08535"
     CDS             complement(1474..3105)
                     /locus_tag="VF276_08535"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX6606945.1"
                     /translation="MAHHLQDHVDLGDSARTLITGRFSRHPERYMEIYRIARKYELHH
                     VAAQFGVGHRHEEEDGPALLPDADGDDHAAGLAAALEELGPCFIKLGQLLSTRPDLLP
                     ANYITALARLQDTIRPVPVEKVIAIVERELGAPVGELFQRFDSAPLATASMAQVHRAM
                     LPDGTDVVVKVQRPGVRNRIEIDLEVLHEMARFATRYTPFGARYGLVQMVRELEHSLR
                     QELDFRQEAENTALISRQIADFTALTTPQVYREYTSPRVLTLSFVQGRHLADVPRADL
                     DARDPRLIGKELLSAYLKQIVIDGIFHCDPHPGNIFLADDGRLALMDFGMVGRFDAGQ
                     KDNIILLLLAFSERQGERVADSYLEMIEIPPRFDRRAFTQDVCALVSRYHDMSGGRMA
                     LGTALLDLTKLAYVHHTPVPSAMTLLGKTMLNLDGTVRVLSPELDPVQLIRDYMIEVM
                     QKRVGAQLSPGRVFAWVLDVKHLVESSPRRTEMILNKLANDQLTVRLELAHLDEMERT
                     VRKAANRLSLGMVASALIIAGGQVYAARRRGNPRD"
     gene            complement(3308..3381)
                     /locus_tag="VF276_08540"
     tRNA            complement(3308..3381)
                     /locus_tag="VF276_08540"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3347..3349),aa:Gln,seq:ctg)
     gene            complement(3493..>3701)
                     /locus_tag="VF276_08545"
     CDS             complement(3493..>3701)
                     /locus_tag="VF276_08545"
                     /inference="COORDINATES: protein motif:HMM:NF015114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX6606946.1"
                     /translation="AVSPLIALLEDSNTDVCAAAVEALGTLKNSRALPTLRRMQQKWA
                     AQGTEGQFLADTARQAIWQIEGHV"
BASE COUNT          518 a         1264 c         1243 g          676 t
ORIGIN      
        1 ccgctcgcgc gctttctgcc cgtgctggcc tggatggctg tcctgggcgg cgcggtcctg
       61 ctgggcacgc ggctgctgcc ggtggccgcg cgaggcgtgc gcgccggcgt gggccgcgcc
      121 ctcgtcctgg tcctgctggt cctgagcagc tacggcctcc aggtcgggtt gctcggcttg
      181 aaggacgcga acccgcaacg gctcctggtc gagcgggtca cgaaccgcgt ctttaccagc
      241 tacttcaccc tggccgcggc gcccacgacg cacctgtcca cctttttccg ggactacccg
      301 gccgtcttca acaccaaact ctgcccccac tgccacgagc acccgcccgg cccggccctg
      361 ttctactggg tcattctgcg cggcatcgcg agcttaccgg agtccctgca acacgacctg
      421 ggcgcggcgg cccggcgcct ggtgggcgac agcccggccc tcgccgcgct ccagcctccg
      481 ctgactgatg cgcaactggt gggcgcggtg gccgggggca acctggtgtt gctgctggcc
      541 gcgacgctcg tgctgccgct gtacggcctg gcgcggctgc tggcgccgcg cgccccgccc
      601 tggtggctgg ccgggctggc cgtggccgtg cccggcgtgc tgctgatggc gccggagttc
      661 gaccaggggc tggggctggt cacggcgggg gcgctctact gcggcttgcg cgggctgcgg
      721 actagccaac cggggcgcgc tgtggcctgg gcggccaccg ccgggctcat cctcgccgtg
      781 gggctgttct tcacctgggc gccggtcgtc ttgttcggcg cgttcgtggg cctgggcttc
      841 ctggcctggg gctacgggcg ttccgcgctg ttcgcgcccg cggacacggg cggacggctg
      901 cccgcccggc aggtggtggc ctggctggcc gccctggtgg cggccacggc gctggtggaa
      961 ctggtgctga cggtgggcct ggggttcgac ttgccctatg tgctgcaata caatctgaac
     1021 aatgccgctt tcgccgaggc ccggcggccc tacgcggtct ggatcttctt cgggccgctc
     1081 gatttcctgc aattcctggg gctgcccctg gcaatcgccg ccctcgccgc gctgatcctc
     1141 cccgcccgcg cagccgccgc gcccgcgccc ggtgcgccgc tggcccgcgt ggcgccctgg
     1201 ctgggccgga tcaatcccta cgcggcgctc ttctgggggc tgctgctggc gatcgatctc
     1261 gccgggcgca ccaaggccga acaggggcgg ctgttgctct acctggcgcc cctggcgctg
     1321 gtggcggtct atgtctgggt tgagcgcacc cgcccggcac cgcgcatcct ggctgccgtc
     1381 ctcgccgccc agttactcgt caccctcgtc ctcggcgcgc gctggttcgt gccgtgagtt
     1441 tgccggcgcc ggcgtcctga cggcgcgccc gcgtcagtcg cgcgggttgc cgcgccggcg
     1501 ggcggcgtac acctgtccgc cggcgatgat cagcgcgctc gccaccatgc ccagtgacag
     1561 ccgattcgcg gccttgcgca cggtgcgttc catctcgtcc aggtgcgcca gttccagccg
     1621 cacggtaagc tggtcgttcg ccagcttgtt caggatcatc tcggtgcggc gcggcgagct
     1681 ttccaccagg tgcttcacgt cgagcaccca ggcgaagacc cgccccggcg aaagctgcgc
     1741 gcccacccgc ttctgcatca cttcgatcat atagtcacgg atgagttgca ccgggtcgag
     1801 ttcgggcgac aggacgcgca ccgtgccgtc gaggttgagc atggtcttgc cgagcagcgt
     1861 catggcactc ggcaccggcg tgtgatgcac ataggccagc ttggtcagat cgagcagcgc
     1921 cgttcccagc gccatgcggc ccccgctcat atcgtggtag cggctgacca gcgcgcagac
     1981 atcctgcgtg aaggcccgcc ggtcgaaccg cggcgggatc tcgatcattt ccaggtagct
     2041 atcggccacc cgctcgccct ggcgctcgga aaaggccagc agcagcagga tgatattgtc
     2101 cttctgcccg gcgtcgaagc ggcccaccat gccgaagtcc atgagcgcca ggcgcccatc
     2161 atcggccagg aagatgttgc cggggtgcgg gtcgcagtgg aagatgccgt cgatcacgat
     2221 ctgcttgagg taggccgaca gcagttcctt gccgatcagg cgcgggtcgc gggcgtcgag
     2281 atcggcgcgc ggcacgtcgg cgaggtgccg gccctgcacg aagctgaggg tgagcacgcg
     2341 cggggaggtg tactcccggt agacctgcgg cgtggtcagg gccgtgaaat cggcgatctg
     2401 gcggctgatc agggccgtgt tttcggcttc ctggcggaaa tccagctcct ggcgcaggct
     2461 gtgctccaat tcgcgcacca tctgcaccag cccgtagcgc gcaccgaagg gcgtgtagcg
     2521 ggtggcgaag cgggccatct cgtgcagcac ttccaggtcg atctcgatgc ggttgcgcac
     2581 gccgggccgc tggaccttga ccacgacatc cgtgccgtcg ggcagcatcg cgcggtgcac
     2641 ctgcgccatc gaggccgtag cgagcggcgc cgagtcgaag cgctggaaca actccccgac
     2701 cggggcgccg agttcgcgct cgacgatggc gatcaccttc tcgaccggca ccgggcggat
     2761 ggtgtcctgc aaacgagcga gcgccgtaat gtagttggcg ggcagcagat ccgggcgcgt
     2821 gctcagcaac tggccgagtt tgatgaagca ggggcccaac tcttccagcg cggcggccag
     2881 acccgcggcg tggtcgtcgc cgtcggcatc cggcagcagg gccgggccgt cctcctcctc
     2941 atgccggtgg cccacgccga actgggccgc gacgtgatgc aactcgtact tgcgggcgat
     3001 ccggtagatt tccatgtagc gctcggggtg ccgggaaaag cggccggtaa tgagcgtgcg
     3061 cgcggagtcc ccaaggtcga catgatcttg cagatgatga gccatagtat gccctcccct
     3121 cccgcccatg ctacggcagg tgggtagcca cgcaccagtt caattggtgc tcactttttg
     3181 ctcatctggt tcccgttgca gcgagtctgc ggcacgcgca tagacttcgg ctacagcggg
     3241 gaatcaaata agccccgcac cattattgtc ggggcttact tacaggctag cgttagccgt
     3301 attcttctgg ctggggagca gggattcgaa ccccaactta ctgatccaga gtcagctgtc
     3361 ctaccgttag acgactcccc atcgcgcttt gcattatacc aaagccagcg gccgttgtca
     3421 acctcgcgca aacgcgcttg gtgagtccat taccgctata accgttgcga ggcagtccgc
     3481 agatggaacg ctctatacat gaccttcaat ttgccaaata gcttggcgcg ccgtatcggc
     3541 caagaattga ccctctgtac cctgagcagc ccatttctgt tgcattcgcc gcagagtagg
     3601 caaggcacga gaattcttca gtgtgcctaa cgcttcgacc gctgctgcac aaacatcggt
     3661 attcgagtct tccagcaaag cgatcagagg cgagacagcg g
//
LOCUS       DASUYC010000520         3872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1333726, whole genome shotgun sequence.
ACCESSION   DASUYC010000520 DASUYC010000000
VERSION     DASUYC010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333726"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /locus_tag="VF276_08550"
     CDS             complement(<1..1031)
                     /locus_tag="VF276_08550"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX6606947.1"
                     /translation="MADDKTKPMALARGAVPPHMTPGQSAGSTGRPRRRRPLWVRLVL
                     GVFVVSLVAVLGYAGTVGYTLWGVSNHIYKPLPTLAPETGTAAPQPAAVPNTPRPGEP
                     TWTPDLAATEEPAEPLPPGRINILVLGTDKRAELGSEAARSDTLILLSVDPKWKTAGI
                     LSIPRDLQVTIPGYGLQKINAAYFFGEYNKIPGGGPALAVPTVSKFFNVPIDYYVSVN
                     FAGFQKIVDEVGGIDVYVPETIDDPEYPGPYNSYIHVHFDAGCTHMDGEEALEYARTR
                     HADSDFGRARRQQQVIRAVRDKALELNMLPRYPELLNQLGDSVETNIPPDKQLAFAQL
                     ASQIKASDI"
     gene            complement(1236..3788)
                     /locus_tag="VF276_08555"
     CDS             complement(1236..3788)
                     /locus_tag="VF276_08555"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HEX6606948.1"
                     /translation="MVGLVALMLFSVIWSVQRAGWSDSLFILTPITLGGMLVGLLLSK
                     IRGVPRLLLHMTGMMAGVALVLWETANLSDDPNLLTIQDRVQDLLSRLVLWVQAVMAG
                     GASDDLQLFLLSLAVLAWVLSYTSTWFVFRSRWLWWAIVPNGVALVVNISYASAQLGW
                     YFILFLMSALLLMVRFNMLLHEERWERERVNYSPSLRWNFLRASSFFALIVAVVMWYM
                     PTQPVNSTLSHAWEQINGPWEDLQDRWSRAFATVNGTGNFGYATFNDSFTLGGALNLG
                     DTVAFNVHSDYPLRWRARTLDSFDGRTWKDTSPATFKGRNVSPRLSLEANQQLPTDDG
                     LRRAVTVTVQMVHPVHDNILLAPLRPISVSVSTRLGVPWQDLNEVYQIPGTNPSDAPI
                     ELQPLLTLFADARRAALQTYGRDPANPTQGGEPLPQGRAEDVARAVLAQVGQDGAIST
                     EIGRLQDRGIVASYFLRNDFTFVVRANGQVPIYDDLNTVYSTDRLQPGESYSVESMVS
                     QADEQTLRQAGTNYPDWVRNRYLELPRDLPVRVIEKAHEVVDQAGASNPYDTAIALQN
                     FLRTYTYNTKIDAPPPGRDAVDYFLFDSKQGYCTYYSSAMVVMLRALGIPAREAIGYA
                     PGDYDAATRQWVVRESASHAWPEVYFPGAGWLEFEPTPSQSVIQRPASPEDVNATPTP
                     FVPTPPGAVATRHALDDESPTPTGGSGAALNTQGGLGSAITPWTLLLLPLLIIAVLLL
                     RYWLERQAVANGRLVLGGLQYYERLVKLAWWLGIRPRPYDTPYEFADQVSEEVPTAGP
                     YVKPIARAYVQERFGRHRPDRAEQQELAQVWAHLRAALLRRMSEARRLLQRR"
BASE COUNT          668 a         1262 c         1311 g          631 t
ORIGIN      
        1 taaatgtcgg aggctttgat ctggctcgcc agttgcgcga acgcgagttg cttatccggc
       61 gggatgttgg tctccaccga gtcgccgagc tggttgagca gttccgggta gcgtggcagc
      121 atattgagtt cgagcgcctt atcgcggacg gcccggatca cctgctgctg gcggcgagcg
      181 cgcccgaagt cgctgtcggc atggcgggtg cgcgcgtatt ccagcgcctc ttcgccgtcc
      241 atgtgcgtgc agccggcatc gaaatggacg tggatgtagc tgttataagg gccggggtac
      301 tccggatcgt cgatggtctc cggtacgtac acgtcgatcc cgcccacctc gtcgacgatc
      361 ttctggaacc ccgcgaaatt caccgacacg taatagtcga tgggcacatt gaagaacttg
      421 ctgacggtgg gcacggccag ggccggaccg ccaccgggga tcttattgta ctcgccgaag
      481 aagtaggccg cgttgatctt ttgcagcccg tagccgggga tggtcacttg caggtcgcgc
      541 gggatgctga ggatgcccgc cgtcttccac ttgggatcga cgctcagcag gatcagcgta
      601 tcgctgcggg cggcttcgct gcccagttcg gcgcgcttat cggtgcccag cacgaggata
      661 ttgatgcggc cgggcggcaa gggttccgcc ggctcctctg tggcggcgag atcaggcgtc
      721 caggtcggtt cgccgggccg gggcgtattg ggcaccgccg ccggctgcgg cgcggcggtg
      781 cccgtttccg gcgccagcgt gggcaacggc ttatagatgt gattcgagac cccccagagt
      841 gtgtagccca cggtcccggc atagcccagc acggccacga gggacacgac gaagaccccc
      901 agcaccagac gcacccagag ggggcggcgg cggcgcgggc gcccggtcga tccagccgat
      961 tggccgggcg tcatgtgcgg gggaaccgcg ccgcgcgcca gtgccatggg tttggtttta
     1021 tcatcagcca taagattcgg tatccaggtt ccggggtctg cgccgcgtac cgagccgcca
     1081 agaccgcgga acacgggtcc cctgagatgc aagggccatg agcaggtaac gggtcgcacg
     1141 cctgcgcacg ggctaattag tcacacaaaa ccacccgccg gggacgaaat gcctacccgt
     1201 ccccggcggg tggtgcaaga aataggccag ggccgttagc gccgctgcaa cagccgccgc
     1261 gcctccgaca tccggcgcag cagcgcggct cgcaggtgcg cccagacctg ggccagttcc
     1321 tgctgttccg cgcggtcggg ccggtggcgg ccaaaccgct cctgcacata ggcgcgcgcg
     1381 atcggcttga cgtacggccc ggcggttggg acttcctcgc tcacctggtc ggcgaactca
     1441 tacggcgtat cgtagggccg cgggcgaatg ccgagccacc aggccagctt gaccaggcgc
     1501 tcgtaatact gcagtccgcc cagcaccagc cgcccgttcg ccacggcctg ccgctccagc
     1561 cagtagcgca gcagcagcac ggcaatgatc agcagcggca gtagcagcag tgtccagggc
     1621 gtgatggccg agccgagccc gccctgggtg ttcagcgccg cgccactccc gcccgtcggc
     1681 gtcggcgact cgtcgtccag cgcgtggcgg gtggcgacgg cgccgggcgg cgtgggcaca
     1741 aacggtgtag gcgtggcatt cacatcctcc ggcgacgccg ggcgctggat cacgctctgc
     1801 gacggcgtgg gctcaaactc cagccagccg gcgccgggga agtagacctc cggccaggcg
     1861 tgcgaggccg actcgcgcac cacccactgt cgcgtcgccg cgtcataatc gcccggcgca
     1921 tagccgatgg cctcgcgcgc cgggatgccg agcgcgcgca acatcaccac catggccgac
     1981 gagtagtagg tgcaatagcc ctgcttgctg tcgaacagga agtagtccac ggcgtcgcgg
     2041 cccggcggcg gtgcgtcgat cttcgtgtta taggtatagg tgcgcaggaa gttctgcaag
     2101 gcgatggccg tatcataggg gttgctggcc cccgcctggt ccaccacctc gtgcgctttc
     2161 tcgatcaccc gcacgggcaa atcgcgcggc agttcgagat aacggttgcg cacccagtcg
     2221 ggatagttcg ttccggcctg gcgcagcgtc tgctcgtccg cctggctgac catcgactcg
     2281 acgctgtacg actcgcccgg ttgcagccgg tcggtgctgt agaccgtgtt caggtcgtcg
     2341 tagatcggca cctggccgtt ggcgcgcacc acgaaggtga agtcgttgcg caagaagtag
     2401 ctggcgacga taccgcggtc ctgcaagcgg ccgatctcgg tgctgatcgc cccatcctgg
     2461 cccacctggg ccagcacggc gcgcgccaca tcttcggccc ggccctgcgg cagcggctcc
     2521 ccaccctgcg tggggttggc cgggtcgcgg ccataggtct gcaacgccgc gcgccgcgca
     2581 tcggcgaaca gcgtcagcag aggttggagc tcgatgggcg cgtcgctggg gttggtcccc
     2641 ggtatctggt agacctcgtt caggtcctgc cagggcacgc ccaggcgcgt ggacacgcta
     2701 acactgatcg gccgcagcgg ggccaggagg atgttgtcgt ggacgggatg caccatctgg
     2761 accgtgaccg tcaccgcccg gcgcagacca tcgtcggtcg gcagttgctg gttggcttca
     2821 agcgagaggc gcggcgagac attgcgcccc ttgaacgtcg cgggggacgt gtccttccag
     2881 gtccgcccgt cgaacgaatc gagcgtacgg gcccgccagc gcagcggata atcgctgtgc
     2941 acgttgaagg ccacggtgtc gcccaggttc agggcaccgc ccagggtgaa ggagtcgttg
     3001 aaggtggcat agccgaagtt gccggtgccg ttgacggtgg caaaggcgcg gctccagcgg
     3061 tcttgcagat cctcccacgg cccgttgatc tgctcccagg cgtggctgag cgtgctgttg
     3121 accggctggg tgggcatata ccacatgacc accgcgacga tcaaggcgaa gaagctggag
     3181 gcgcgcagga agttccagcg caggctgggc gagtagttga ctcgctcgcg ctcccagcgc
     3241 tcctcatgca gcaacatgtt gaagcgcacc atcagcagca gtgcgctcat caggaacagg
     3301 ataaagtacc agcccaactg ggcactggca taggagatgt tgacgaccag cgccacgccg
     3361 ttgggcacga tcgcccacca gagccagcgc gaccggaaca caaaccaggt gctggtatag
     3421 gacagcaccc aggccagcac cgcgagcgac agcaggaaca actgcaggtc gtcgctggcc
     3481 ccgcccgcca tcaccgcctg gacccagagc acgagccggc tcagtaggtc ctggacgcgg
     3541 tcctggatgg taagcaggtt cgggtcgtcc gagaggttgg ccgtctccca gagcacgagc
     3601 gccaccccgg ccatcatgcc ggtcatatgc agcagcaggc gcggcacccc gcggatcttg
     3661 ctcagcagga ggccgaccag catcccgccg agggtgatgg gggtcaggat aaacaggctg
     3721 tcggaccacc cggcacgctg aacggaccag atcacgctga acagcatcag cgccaccagg
     3781 ccgaccatca gccatccttc gcgtagtcga aagtattttg tcatagcgca atctagcccc
     3841 agccagggat ttctatgcag tagaacgcgc cg
//
LOCUS       DASUYC010000521         3293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_792733,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000521 DASUYC010000000
VERSION     DASUYC010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792733"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VF276_08560"
     CDS             <1..293
                     /locus_tag="VF276_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606949.1"
                     /translation="VLGTAGFAAAGWRAGLLPRWNALPVLGGGLAAIAMLLLSIGGYD
                     LMTEDQRNLLNLIALSGLVVFMAGWIMIHHRLWALVGNPRPGAVSRSDVVAG"
     gene            384..1451
                     /locus_tag="VF276_08565"
     CDS             384..1451
                     /locus_tag="VF276_08565"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEX6606950.1"
                     /translation="MHRLLYAMLALMLVLLAGCGIAADPPPAPPVAAATAVPRLPDAR
                     PVIIDTDMAADDWLAILYLLQRPDIAVQAITVSGTGEAHCGPGVTNARGLLALAGYPA
                     LPVSCGRETPLRGDHVFPAAWREGSDALAGLRLPAATGPAETRPAPALLAAVIEAAPS
                     PVTLLALGPLTNVAEALQSTPGLGAKLAGITIMGGAVTVPGNIAESAVPVANPHAEWN
                     LYIDPYAASIVFQSGVPLTLIPLDATNHVPVNPAFYARIKAQHTTPAATFAYDLLTKN
                     PDFVRSGEYWFWDSLTAATLTDPALVTFQEMRLRVVEAEGPESGRTLSVADGVPVRVA
                     VSAVAPEFERIFLATLNGQAP"
     gene            1655..2776
                     /locus_tag="VF276_08570"
     CDS             1655..2776
                     /locus_tag="VF276_08570"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX6606951.1"
                     /translation="MLKTWSVAPMPALSLRAGIVALLLLGSLAAGYWLGWQPPVVFLL
                     FVPVALAGWWFRYEIALAVAVLATLLPALAERLAGAPGLNGAGWVGVLLAGALLARVT
                     FLERTRRRQAEAQAAAYAALEARCQDLEEVYRRQGRFLQIAAHEFKTPLTIILGVVDL
                     IQLQGARMSEERLRALVATMPLAGRQLRLLIDNLLDQGRLERGSLDLAQDLIDFPLLV
                     REAVAGVAVGADAGRVSVDCSEGLPEVYGDAPQLLRAVVNLVSNALKYSPPDSPVQVR
                     VAPRGAGVALAVVDEGPGIALTEQERIFDPYYRGPAAATARSAGSGLGLSIVRETVRR
                     FGGRVEVQSALGAGSTFTVWLPASGERACPPDTAELRGT"
     gene            2781..>3293
                     /locus_tag="VF276_08575"
     CDS             2781..>3293
                     /locus_tag="VF276_08575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03717.1"
                     /note="Members of this highly hydrophobic protein
                     family,regularly are found preceded by the yybP-ykoY
                     manganese riboswitch (see RF00080). A metal cation
                     transport function is proposed; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjbE family putative metal transport protein"
                     /protein_id="HEX6606952.1"
                     /translation="MDGFIGALLGIVLIDLVLSGDNAIVIGMAVRNLPPHQKRVAILG
                     GTAGAVILRVILTALAALLLRVPLLQAAGGLVLVWITYRLLSPSGHGDSADHSGDSFG
                     QALRMIILADVSMSIDNVLAVGAAAQGDLVLLLFGLSLSLAIIMAGGSVVATLLGKLP
                     WLIYIGGGVLL"
BASE COUNT          427 a         1105 c         1195 g          566 t
ORIGIN      
        1 tggtgctcgg cacggcgggg ttcgccgccg ctgggtggcg cgccggcctg ctgccccgct
       61 ggaacgcgct gcccgtgctg ggcggtgggt tggccgcgat cgcgatgctg ctcctcagca
      121 ttgggggcta tgacctcatg accgaggatc agcgcaatct cctgaacctg atcgccctga
      181 gcggcctcgt ggtctttatg gcgggttgga tcatgatcca ccaccggctc tgggccctgg
      241 tggggaaccc ccgccccggc gcagtctccc ggtccgatgt ggttgccggc taggcgccgt
      301 cggaccgatg tcagcgggca ccgcgggcgg atcggcgcaa cgcgccggtg cgcccggtgc
      361 ccaggaaagg agtgtaacta ctagtgcacc ggttactata tgcaatgctt gcgctgatgc
      421 tcgtcctgct ggcgggctgc gggatcgcgg cggacccgcc gcccgcgccg cccgtcgcgg
      481 ccgcgacggc tgttccccgg ctgcccgacg cccgcccggt gatcatcgac accgacatgg
      541 cggcggacga ctggctggcg atcctttatc tgctgcaacg cccggatatc gccgtccagg
      601 cgatcaccgt cagcggcacg ggcgaggccc attgcgggcc gggcgtgacc aatgcgcgcg
      661 gcctgctggc gctggccggc taccccgcgc tcccggtgag ttgcgggcgc gagacgccct
      721 tgcgcggtga ccacgtcttc cccgccgcct ggcgcgaagg ctcggacgcg ctggcaggcc
      781 tgcgcctgcc ggcggctacg ggtccggcgg agacccgccc ggcgcccgcg ctgctcgcgg
      841 cggtgatcga ggccgccccg agccccgtca cgctgctggc cctggggccc ttgaccaacg
      901 tggcggaggc cctgcaaagc acgcccggcc tgggcgcgaa gctggcgggc atcactatca
      961 tgggcggcgc ggtgacggtg cccggcaaca ttgccgagtc cgcggtcccc gtcgccaacc
     1021 cccacgcgga gtggaatctc tacatcgatc cgtatgcggc gagcatcgtg ttccagagcg
     1081 gggtgccgct cacgctgatc ccactggacg cgaccaacca tgtcccggtc aacccggcgt
     1141 tttatgcccg catcaaagcc cagcacacca cgccggcggc caccttcgcc tacgacctgc
     1201 tgacgaaaaa ccccgatttc gtccggtccg gtgagtactg gttctgggac tccttgacgg
     1261 cggcgaccct caccgatccc gccctggtca cgttccagga gatgcgcctg cgggtggtcg
     1321 aggccgaggg gccggaaagc gggcgcacgc tgtccgttgc cgacggcgtg ccggtgcggg
     1381 tggccgtatc cgccgtcgcg ccggagtttg agcggatctt cctcgccacc ttgaatgggc
     1441 aggcgccgtg atgggatcca acggcggcat gggaatcgcc tcttagcaaa ttctcaggcc
     1501 cctctcaggg ccctctcagg attcaccgct atactgtaag cataccgtcg cggacgagcc
     1561 cggtgctcgc ttcgcgacgt ggctatttta ggggcaaaag aaccggtgtg cccccgattc
     1621 cggcgcggat gggcgtctta tgaggagcgt agcgatgctg aagacatggt ccgttgcgcc
     1681 catgccggcc ctgtcgctgc gcgccggaat cgtggcgctg ttactcctcg gcagcctggc
     1741 ggcggggtat tggctgggct ggcagccgcc cgtggtgttc ctgctctttg tgccggtggc
     1801 cctggccggg tggtggttcc gctacgagat tgcgctggcc gtcgccgtgc tggcgaccct
     1861 gttgccggcc ctggccgagc gcctggccgg ggcgccgggc ctcaacggtg ccggatgggt
     1921 cggtgtactg ctggccgggg cgctgctggc gcgggtgact ttcctggagc ggacccggcg
     1981 ccggcaagcc gaggcccagg ccgccgccta cgcggccctg gaagcgcgct gccaggatct
     2041 cgaagaggtc taccgccgcc agggccgctt cctgcagatc gccgcgcacg agttcaagac
     2101 gccgctgacg atcatcctgg gcgtggtcga tctgatccag ttgcaagggg cgcgtatgtc
     2161 cgaggagcgg ctacgcgccc tcgtcgcgac gatgccgctg gccgggcggc agttacgcct
     2221 gctgatcgac aatctgctcg accagggccg gctggagcgg ggttcgctgg acctggccca
     2281 ggacctgatc gatttccccc tgctggtgcg cgaggccgtg gccggggtgg cggtgggggc
     2341 cgacgcgggg cgggtcagcg tggactgctc cgagggcctg cccgaagtct acggcgatgc
     2401 gccgcagttg ctgcgcgcgg tggtcaacct ggtcagcaac gcgctcaagt actcgccgcc
     2461 ggacagcccg gtgcaggtgc gggtcgcccc gcgcggggcg ggggtggcgc tggccgtggt
     2521 cgatgaaggg ccgggcattg cgctgacgga gcaggaacgc atcttcgatc cctattaccg
     2581 cggtccggcc gcggcgaccg cgcgttcggc gggcagcggc ctgggcttga gcatcgtgcg
     2641 cgaaacggtg cggcgcttcg gcgggcgcgt cgaagtgcag agcgcgctag gcgcgggcag
     2701 cacgttcacc gtctggctcc ccgccagcgg ggaacgtgcg tgcccgcctg atactgcaga
     2761 actcagaggt acttgacata atggatggat ttatcggcgc cctgctgggc atcgtcttga
     2821 tcgacctcgt cttgagcggg gataacgcca tcgtcatcgg catggccgtg cgcaacctgc
     2881 cgccgcacca gaagcgggtc gccatcctgg gcggcacggc aggcgcggtg atcctgcggg
     2941 tgattttgac ggcgctggcg gcgttgctgc tgcgcgtgcc gctgttgcag gccgcgggcg
     3001 gactggtgct cgtctggatc acctaccggc tgctcagccc ctccgggcac ggggacagtg
     3061 cggaccattc cggcgactcg ttcggccagg cgctacgcat gattatcctg gccgacgtat
     3121 cgatgagcat cgacaacgtg ctggcggtgg gcgcggcggc gcagggcgat ctggtgctgc
     3181 tgctgttcgg cctcagcctc agcctggcga tcatcatggc cgggggcagc gtggttgcca
     3241 cgctgctcgg caagctgccc tggctgattt atatcggcgg cggggtgctg ctg
//
LOCUS       DASUYC010000522         3349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1181019, whole genome shotgun sequence.
ACCESSION   DASUYC010000522 DASUYC010000000
VERSION     DASUYC010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181019"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2353
                     /locus_tag="VF276_08580"
     CDS             <1..2353
                     /locus_tag="VF276_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606953.1"
                     /translation="RPRAYWTALPAPAPLGAAGGGTYQPASWQILYGDPAGTAATAAV
                     DPASRNVPDARFYAELVGGRVPAGLFIHMDEAQDGYAFIIRPERRNTGWWRVSHGVPT
                     TLIDGDVYRPSFWAGLSDLVQELAIALTGALLLVAVVGGLALLARALLGRWGMAPGTA
                     PVLDAASDDLLGTEHADDKRVRRDKSRNYTQEVAGTGTGQADPPESAASWRHWLPALA
                     FALAGLVAAAAICLGPLQGIPHVQDDVAYVWQAKIFALARAWVPAPPRPYFFEQGFIL
                     ITDGRWFTKYPPGWPLLLVPGIWAGIPWIVDPLCAGGTLALVYATGRRIYGPAAGFWA
                     ALLGLTSPFLMFMAGSFMSHTSTMFFLMLAVYCFVWLKTAPPAPAAARPILGLDWRTV
                     LLSLGTGFGLSWAFISREATALGVALPFFVWSLVDITGGLRAWLRARRAAPGGLRPLI
                     TRIAPYALMVAGALPPLVVLGVVNQALLGSPFKLAQELVGSYDRLGFGPGFGPEPAGH
                     TPALGLYNALVYLRTLQGDLFGWPPTLALAPLLIALAGTVGSRWRRARWDLFLLGGFL
                     GLVAIYFAWWSSTTIYGARYWYEALPFLLLLSGRGVQLLGRGAEVLLAAYSNGRQAGL
                     KPAPTAQPGQQAGLKPAPTAQNHSNGLPGSGVLSGRRAAWAALAVPALAVGLLIVFNL
                     AQVMPGQVRAYTGYNDVDASALQRVEAAHLNHAVVFVALNPAFPRRDYGKVFFANDPL
                     LRGPVVYARDVGAGGNAALLSSFPDRQPYYLPLDGPLQPGTGP"
     gene            complement(2370..3227)
                     /locus_tag="VF276_08585"
     CDS             complement(2370..3227)
                     /locus_tag="VF276_08585"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009057 - macromolecule catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HEX6606954.1"
                     /translation="MAPGAQAGDAGAPPRGYLLIVGGSERLDKELRLLRTYLQLCDQA
                     PGRRDQVIITSATGHPEALGGEYWRIFRELGWPDRLIHMPPLATREQASDPGVAALVA
                     QAAGIWMTGGDQVDLVDILQGTPVGDAMLTAYQEGAVVGGTSAGATAIGNPMIARGGG
                     SGEVKPGEIRLHPGFGFTGSDMLIDTHFGRRGRFPRLVAAIAEYPAAVGVGIDENTAL
                     LVRPDTPDAEILGSGVVYLIENPHPPENWDPHHGTVGVTPLTLYSLREGHRYEIGARS
                     STAVAGSGS"
BASE COUNT          397 a         1178 c         1227 g          547 t
ORIGIN      
        1 ccggccccgc gcctactgga cggccctgcc cgcgcctgcc cctttagggg cagccggcgg
       61 cggcacctac cagcccgcgt cctggcagat tctctacggc gaccccgcgg gcaccgcggc
      121 caccgccgcc gtcgatcccg ccagccgcaa cgtcccggac gcgcgcttct atgccgaact
      181 ggtgggcggg cgggtcccgg cggggctgtt catccacatg gacgaggcgc aggacggcta
      241 tgcctttatc atccgccctg agcgccgcaa caccggctgg tggcgcgtga gccacggcgt
      301 gcccaccacg ctgatcgacg gcgatgtcta ccgccccagc ttctgggcgg gcctgtcgga
      361 cctggtgcag gaactggcaa ttgctctcac gggcgcgctg ctgctggtcg cggtcgtggg
      421 cgggctggcg ctgctcgccc gcgcgctgct ggggcggtgg ggcatggcgc cgggcacggc
      481 ccccgtgctt gatgccgctt ccgacgatct attggggaca gaacacgctg acgacaaacg
      541 agtacggcgc gataaatcgc gcaactacac ccaagaggta gctggaaccg gcaccgggca
      601 ggcggacccg cccgaatccg ccgcctcatg gcggcactgg ctgccggcgc tggcctttgc
      661 gctggccggg ctggtggcgg cggcggcgat ctgcctgggg ccgttgcagg gcatccccca
      721 tgtgcaggac gacgtggcct atgtctggca ggctaagatc ttcgcgctgg cccgcgcctg
      781 ggtgcccgcg ccgccgcgcc cctacttctt cgagcagggc ttcatcctga tcaccgacgg
      841 gcgctggttc accaagtatc cgccgggctg gccgctgttg ctggtgccgg gcatctgggc
      901 gggcatcccc tggatcgtcg acccgctctg cgccgggggc acgctggccc tggtctacgc
      961 caccgggcgg cggatctacg gcccggcggc ggggttctgg gcggcgctgc tcggcctgac
     1021 ctcgcccttc ctcatgttca tggccgggtc gttcatgtcg cacacctcga ccatgttctt
     1081 cctcatgctg gccgtctact gcttcgtatg gctgaagacg gcgccgcctg ccccggcggc
     1141 ggcccggccc atcctggggc tggattggcg caccgtgctg ctgagtctcg gcacgggctt
     1201 cgggctgtcc tgggcgttta tcagccgcga ggccacggcg ctgggcgtgg cgctgccctt
     1261 cttcgtctgg agcctggtgg acatcacggg tgggctgcgg gcctggctgc gcgcccgccg
     1321 cgcggccccc ggcggcctcc ggccactgat cacgcgcatc gcgccctacg cactcatggt
     1381 ggcgggggcg ctgccgccgc tggtggtgct gggcgtggtc aaccaggccc tgctcggctc
     1441 gcccttcaag ctagcccagg aactggtggg cagctacgac cggctgggct tcggccccgg
     1501 ctttgggccg gaaccggcgg gccacacgcc cgcgctcggc ctctacaacg ccctggtcta
     1561 tctgcgcacg ctgcaaggcg acctgttcgg ctggccgccc acgctggctc tggcgccgct
     1621 gctgatcgct ctggccggca cggtgggcag ccggtggcgg cgggcgcgct gggacctgtt
     1681 cctgctcggc gggttcctgg ggctggtcgc catctacttc gcctggtggt cctcgaccac
     1741 gatctacggg gcgcgctact ggtacgaggc gctgccgttc ctgctgctgc tgagcgggcg
     1801 gggggtgcaa ttgctggggc ggggcgcgga ggtgttgctg gcagcctact caaatgggcg
     1861 gcaggccggt ttgaaaccgg cccctacagc gcaacctggg cagcaggccg gtttgaaacc
     1921 ggcccctaca gcgcaaaatc acagtaacgg gctaccaggc agcggcgtcc ttagtgggcg
     1981 ccgggctgca tgggcggcgc tggccgtccc ggcgctggcg gtgggactgc tcatcgtgtt
     2041 taacctggcc caggtgatgc ccggccaggt gcgcgcctac accggctaca acgacgtgga
     2101 cgcctcggcc ttgcagcgcg tcgaagccgc gcacctgaac cacgccgtgg tgttcgtcgc
     2161 cctcaacccg gccttccctc gccgcgacta cggcaaggtc ttcttcgcca acgacccgct
     2221 gctgcgcggc ccggtggtct acgcgcgcga tgtcggtgcg ggcggcaacg cggcgctgct
     2281 gtcgagcttc ccggatcgcc aaccgtatta cctgccgttg gacggccccc tccagccggg
     2341 caccgggccg tgagctgggc gtgccgccgt caggacccgc ttccggcgac ggcggtggaa
     2401 ctgcgcgcgc cgatctcgta gcggtggcct tcgcgcaggc tgtagagggt gagcggggtc
     2461 acgccgacgg tgccgtggtg cgggtcccag ttctcgggcg ggtgggggtt ttcgatcagg
     2521 tagacgacgc cgctgcccag gatctcggcg tcgggcgtgt cggggcgcac caacagggcg
     2581 gtgttctcgt cgatgccgac gccgaccgcc gcggggtact cggcgatggc cgccacgagg
     2641 cgggggaagc gcccgcgccg gccaaagtgg gtgtcgatca gcatatcgct gccggtgaag
     2701 ccgaaaccgg ggtgcaggcg gatctcgccc ggtttcacct cgccggagcc gccgccgcgg
     2761 gcgatcatcg ggttgccgat ggccgtggcc ccggcgctgg tgccgccgac caccgcgccc
     2821 tcctggtagg ccgtgagcat ggcgtcaccg acgggcgtgc cctggagaat gtccaccaga
     2881 tccacctggt cgccgccggt catccagatg cccgccgcct gtgcgaccag ggcggccacg
     2941 ccgggatcgg acgcctgctc gcgggtcgcc agcggcggca tgtggatcag gcggtccggc
     3001 cagcccagtt cgcggaagat gcgccagtac tcgccaccga gcgcctcggg gtgcccggtg
     3061 gccgaggtga tgataacctg gtcgcggcgg cccggcgcct ggtcgcacaa ctggagatag
     3121 gtgcgcaaca ggcgcagctc tttgtccagg cgctccgagc cgccgacgat cagcaggtag
     3181 cccctgggcg gggcgccggc gtcgcccgcc tgcgcgccgg gggccactac agattgtcct
     3241 cgccgtcggt gtaacgggcg gtggtgccgt tgccgggcgc cgggggcgtg ccgttgttca
     3301 gcaccgtggt gcgggccagg ggcagggcct ggcggctatg caccgggtg
//
LOCUS       DASUYC010000523         3230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1479939, whole genome shotgun sequence.
ACCESSION   DASUYC010000523 DASUYC010000000
VERSION     DASUYC010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1479939"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..1789
                     /gene="alkA"
                     /locus_tag="VF276_08590"
     CDS             293..1789
                     /gene="alkA"
                     /locus_tag="VF276_08590"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HEX6606955.1"
                     /translation="MHTNHTPTTNLPLNADNCYQALVSRDARFDGRFFVGVSSTRIYC
                     RVVCPAKTPRRENCTFYPSAAAAERAGYRPCLRCRPELAPGNAVLDAVSRLAAEAASR
                     IEDGVLTEASVGHLAAELGISDRHLRRVVEREFGVTPIELAQTHRLLLAKRLLTDTDL
                     PVIEVAYASGFSSLRRFNALFKERYRLNPTGLRKAQPHRIAQRPLVCDLAYRPPLDWV
                     ALLGFLSARTITGVEQIDGLCYRRTAAFGKHRGWLAVDPVPGKALLRISLAPTLAPVL
                     PQVLAAVKRLFDLLAPPDLIATHLGALAVANPGLRVPGAFDGFEVAVRAILGQQVSVK
                     AASTLAGRLSAAFGEAVETPYAELAVLTPGAARLAGAAPEEIMALGVTRSRATAIVAL
                     AQAVAAGAIRLEPRVDVAEAMARLRDLPGVGEWTAQYVAMRALSWPDGFPHGDLGIRK
                     ALNEPNPNRVFALAEAWRPWRAYAAMHLWKSLETPACLTELEPQPQIA"
     gene            1750..2385
                     /locus_tag="VF276_08595"
     CDS             1750..2385
                     /locus_tag="VF276_08595"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020554097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX6606956.1"
                     /translation="MFDRTRTATADRVIYYTTVRSPIGPLLLTSDGTALTGLHMSPLP
                     PRPPTGDVAQDWDLPDAGGPWVADADAAPLPAAAAQLAAYFAGRLTAFDLPLALHGTE
                     FQRRVWTALLAIPYGTTISYGDLARWVGNPQGSRAVGLANGRNPIAVIVPCHRVIGAN
                     GKLTGYGGGLPRKAALLDFEFGVRLHGPAAFPPLADPAPLAPREPALAAAR"
     gene            2576..2800
                     /locus_tag="VF276_08600"
     CDS             2576..2800
                     /locus_tag="VF276_08600"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6606957.1"
                     /translation="METLTWHTHHNDMPDLRHEEYTVAELAEILGVGRELLLHAIRTG
                     ELKAMQVHSHAIIIKREDALDWLKRRGQLF"
     gene            2842..>3230
                     /locus_tag="VF276_08605"
     CDS             2842..>3230
                     /locus_tag="VF276_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6606958.1"
                     /translation="MIKTRGLTKTFPRGKGEAPVLAVDHLDLDVGQGEVFGFLGPNGA
                     GKTTTVRMLTSLIGPTAGEASVAGFRVGQDDTDIRRNAGILTESPGLYERLSAWTNLE
                     IFAHLYEVKDVPGQVEKYLRMLGLWDRR"
BASE COUNT          482 a         1136 c         1121 g          491 t
ORIGIN      
        1 gcgccctacg atgtgctact ggcccaactc ggcaccgcac gctatcaggc ccggtagccg
       61 gaccgctggg gagccggtca cgcgatcggc gcggaccccg taggcggtat ccgccgctta
      121 cctgaccggc tctcccgact aattgctgcg actactcgtt ctgatcccgc cgcacggccg
      181 ggcaccgtca ccgcccatcc gtgcggcggg ccgcttcgta tatccccaac cgggcggctt
      241 gcatgtccga aaacggcgag aaagtgcgcg ggaattgcgc tacaatgggg acatgcatac
      301 gaaccacacc cccaccacca acctgccgct gaacgcggac aattgttacc aggcgctggt
      361 cagccgggac gcgcgcttcg acggacggtt cttcgtcggc gtgtcgagca cgcgcattta
      421 ctgtcgtgtc gtgtgcccgg ccaagacgcc acggcgcgag aactgcacgt tctatcccag
      481 cgcggcggcg gcggagcggg cggggtatcg gccctgtttg cgctgccggc ccgaactggc
      541 gcccggcaac gccgtgctcg atgccgtgag ccggctggcc gccgaggctg cgagccgcat
      601 cgaggacggc gtgctgaccg aggcgagcgt cggccacctg gccgccgaac tcggcatcag
      661 cgaccggcac ctccgccgcg tggtggagcg cgagttcggc gtcacgccga tcgagctggc
      721 gcagacgcac cgcctgctgc tggcgaagcg cctgctgacc gacaccgacc tgccggtgat
      781 cgaagtggcc tatgccagcg ggttttcgag cctgcgacgc tttaacgcgc tgttcaagga
      841 acgttaccgg ctgaacccca cgggcctgcg caaggcccag ccgcaccgca tcgcgcagcg
      901 cccgctggtc tgcgacctgg cctaccgccc gccgctggac tgggtggcgc tgctcggctt
      961 cctgagcgcg cggacgatca ccggagtgga gcagatcgac ggcctgtgct accggcgcac
     1021 ggcggcgttc ggcaagcacc gcgggtggct ggcggtggac ccggtgccgg ggaaggcgct
     1081 gctgcggatc agcctggcgc ccacgctggc cccggtgctg ccgcaggtgc tggccgccgt
     1141 caagcggctg ttcgacctgc tggccccgcc cgacctcatc gccacgcatc tcggcgcgct
     1201 ggccgtggcg aatcccggcc tgcgggtgcc gggcgcgttc gacggcttcg aggtggcggt
     1261 gcgggccatc ctgggccagc aggtgagcgt caaagcggcg agcaccctgg cgggccgcct
     1321 ctccgccgcc ttcggcgaag ccgtcgagac gccgtatgcg gagctggccg tgctcacacc
     1381 cggagcggca cggctcgccg gggcggcgcc ggaggagatc atggcgctgg gcgtcacgcg
     1441 cagccgggcc acggccatcg tggccctggc ccaggccgtg gcggcgggcg cgatccggct
     1501 ggagccgcgg gtcgacgtgg ccgaggcgat ggcccggctg cgcgatctgc ccggcgtggg
     1561 cgagtggacg gcgcagtatg tcgccatgcg cgccctgtcc tggccggacg ggttcccgca
     1621 cggcgacctg ggcatccgca aggcgctgaa tgagcccaat cccaatcgcg tgtttgcgct
     1681 ggcggaagcc tggcgcccct ggcgcgccta cgccgcgatg cacctatgga aatcactgga
     1741 gacccctgca tgtttgaccg aactagaacc gcaaccgcag atcgcgtgat ctattacacc
     1801 acggtgcgca gcccgatcgg gccgctgctg ctgacctcgg acggcacggc gttgaccggg
     1861 ctgcatatga gccccctgcc gcctcgcccg ccgaccggcg atgtggccca ggactgggac
     1921 ctgccggacg ccggcggccc ctgggtcgcg gacgcggatg ccgcgcccct gcctgcggcg
     1981 gcggcgcaac tggccgccta tttcgcgggc cggctgacgg cgtttgatct gccgctggcg
     2041 ctgcacggca ccgagttcca gcgccgggtc tggaccgcgc tgctggccat cccctacggc
     2101 accacgatct cctatggcga cctggcccgg tgggtcggca atccgcaggg atcgcgggcg
     2161 gtggggctgg cgaacgggcg caacccgatt gccgtcatcg tgccctgcca ccgcgtgatc
     2221 ggggctaacg gcaagctgac gggctacggc ggcggcctgc cccgcaaggc cgcgctgctc
     2281 gacttcgagt tcggcgtgcg gctgcacggt cccgccgcgt tcccgcccct ggccgatccc
     2341 gccccgctcg ccccccgcga gccggcgctg gccgccgctc gctagccact ggctgagacc
     2401 gcgccgggca tctcccccgg cgcggtcttt cttcacgccc gccccgcgct tttaacccat
     2461 ctgtcacgca tatataccgt tgcgccaacc gctgtcggct atgatgaaac ggggaaccgc
     2521 tagccgggta ttgcggagcg ccgggcggct gccggcccct ggaaggagag gatcgatgga
     2581 gaccctaacc tggcatactc accacaatga catgcccgac ctgcgccatg aagagtacac
     2641 ggtggccgaa ttggcggaga tcctcggtgt gggccgtgaa ctgctgctgc atgctatccg
     2701 gaccggcgag ttgaaggcca tgcaggtgca ttcgcacgcc atcatcatca agcgcgaaga
     2761 cgcgctggat tggctgaaac ggcggggaca gttgttttaa gccaacccgg ttttcaccac
     2821 gggttgcggc aaggaacact catgatcaaa accagaggct tgaccaagac gtttccgcgg
     2881 ggcaagggag aggccccggt gctggcggtt gatcacctgg atctcgacgt gggccagggc
     2941 gaggtgttcg gcttcctcgg gccaaacggc gcgggcaaaa ccaccaccgt gcgcatgctg
     3001 acctccctca tcggccccac cgcgggcgag gcgagtgtgg ccggcttccg ggtggggcaa
     3061 gacgacacag acatccggcg caacgccggc atcctgaccg agtcgcctgg cctctatgag
     3121 cggttgagcg cctggaccaa cctggagatc ttcgcccacc tctacgaagt gaaggacgtg
     3181 ccgggccagg tggagaagta cctgcgcatg ctcgggctgt gggaccggcg
//
LOCUS       DASUYC010000524         3614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_306091,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000524 DASUYC010000000
VERSION     DASUYC010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306091"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VF276_08610"
     CDS             complement(<1..340)
                     /locus_tag="VF276_08610"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6606959.1"
                     /translation="MNTAVTFRWFGVAGVAITAQGQTLAIDPFFSRPPFLRFFVGHPT
                     PDPVLDARYLPVCDYVLVSHAHWDHLMDVPSVVRNTGAIAFGSPNSCRLLALLGLPPA
                     QIRLLRLGDHL"
     gene            complement(337..1263)
                     /locus_tag="VF276_08615"
     CDS             complement(337..1263)
                     /locus_tag="VF276_08615"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6606960.1"
                     /translation="MLPIAAPPPAGGTSPRSRIFTLPDGRALAFAEWGDPAGHPIFLF
                     HGTPGSRLTRPADEAPARARGVRLITVDRPGYGRSDAQPGRQLLAWPADIAALADALH
                     LATWAVVGVSGGGPHALACTYQLPHRVAAVALVGGVAPLDQAGAQEQMLRVYRLAIRL
                     SNHLPKWPARGPARVGSGLMRRYPHVAIPLLTARLPAVDRAMLTRPAVLNASVETIAE
                     AFRQGIQGVVGDIAVLAHPWGFNPAAIRRPVYLWHGVADRNVPVAHGRYLAATIPACR
                     ATFCPAEGHELLYDHWPAILDALLPHCRGPYV"
     gene            complement(1272..1994)
                     /locus_tag="VF276_08620"
     CDS             complement(1272..1994)
                     /locus_tag="VF276_08620"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX6606961.1"
                     /translation="MPLQQGPAAPARWDQKALRLLVRTLFRLLFRVRVVGLDNIPPTP
                     VIICANHLGWADPFLILLFFPAEPRIYVLGMEAIYETTPLVRRILDWLQIMVALDPQQ
                     PQCAARAMEEMVAHGGRLLIFPEGRLGDAEGRLQSLHPGAAHAALHNHVPLLPVGLTG
                     THELWLRRPLTMRIGPPIIPETAPGPLHARVAALIERLTAAMQALLPGDAVADRRKPL
                     CVWLMQMDRRVAPAADHGTEGG"
     gene            complement(2493..2975)
                     /locus_tag="VF276_08625"
     CDS             complement(2493..2975)
                     /locus_tag="VF276_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606962.1"
                     /translation="MSLHYEFMLAFDLKPDTPPDIIALLQYLTRTEDYAYDGPPPEPV
                     IGYAPDAGDPWWRQMLQNRDPLWYGAGSAIGILAAAPSWRRNNAQHTLTIRSEALDDD
                     FYQVWLFMFVWLAQYSATEGWVGYYREQADMQPTLIYFRDGQVYYYDIPPTAPLQPVA
                     "
     gene            complement(3002..>3614)
                     /locus_tag="VF276_08630"
     CDS             complement(3002..>3614)
                     /locus_tag="VF276_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606963.1"
                     /translation="AALGGGAAPPQLAMQINAFLAAVVLVMAALLFLELSLSTLSRQG
                     RAWSILRLAPIGGASILRGWFLALALPFVLLSSALLGVVGLWRGFSLGGFLYGWGGVE
                     LIGLSMLAVTLSMGVVLARPDPAGPLLDGGARLLGELLALALGGLTGGILCLPLVVAA
                     QTESPAPLWLWLAAGAGAALIAGAGSGLALLVAHRRLPRAGER"
BASE COUNT          670 a         1127 c         1307 g          510 t
ORIGIN      
        1 ggagatggtc cccgaggcgc agcagccgga tctgcgcggg cggcaagccg agcagggcca
       61 gcaaacggca gctattcggc gacccgaagg cgatggctcc ggtgttgcgc accacgctgg
      121 gtacatccat caggtgatcc caatgggcat gggagaccag tacatagtcg cacacgggca
      181 ggtagcgggc atccagcacg ggatcgggtg tcgggtgccc cacaaagaag cgcaggaacg
      241 gcggccggct gaagaaggga tcaatggcca gggtttgccc ctgggctgtg atggccactc
      301 cggcaacgcc gaaccagcgg aaggtgacgg cggtattcat acgtatggcc ctcggcaatg
      361 aggtagcagt gcgtccagga tcgcaggcca gtgatcgtac agtaattcgt ggccctcggc
      421 gggacagaaa gtggcccggc aggcgggaat ggtggccgcc aggtaccgcc cgtgggcgac
      481 gggcacgtta cggtcggcga caccgtgcca cagatagacc ggcctccgga tggcggcggg
      541 gttgaagccc cagggatggg ccagcacggc gatgtccccc accacgccct gtatgccctg
      601 gcgaaacgct tcggctatcg tctccaccga agcgttcagg acagcgggcc gggtgagcat
      661 ggcgcggtcg acggcaggca accgcgcagt gagcagcggg atggccacat ggggatagcg
      721 ccgcatgaga ccagagccca cgcgggcggg ccccctcgcc ggccacttcg ggagatgatt
      781 cgacaggcgt atggccaggc gatagactcg cagcatctgc tcctgtgcac cggcctggtc
      841 tagcggtgcg acgccgccga ccagggcgac ggcggccacg cggtgcggga gttggtaggt
      901 gcaagcgaga gcgtgcggcc cgccgcccga gactcccacg acggcccacg ttgccaggtg
      961 gagggcatca gccagggcag cgatatcggc gggccaggcg aggagttggc ggccgggttg
     1021 ggcatccgag cgaccgtagc ccggccggtc tacggtgatc aggcgtaccc cgcgggcacg
     1081 ggctggcgcc tcgtcagcag ggcgggtgag ccgggaaccg ggcgtgccat gaaacaagaa
     1141 gatcggatgc cctgccgggt cgccccattc ggcaaatgcc agggcccgcc cgtcggggag
     1201 ggtgaagatc cggctgcggg gggatgtgcc gcccgccggg ggcggagccg cgatcgggag
     1261 catgatcctc ctcaccctcc ctcggtgcca tggtcggctg ccggcgccac ccggcgatcc
     1321 atttgcatca accagacgca gagcggcttg cggcggtctg caaccgcatc gcccggcagg
     1381 agggcctgca tggctgccgt caggcgctcg atgagcgccg caacgcgcgc atgtagcgga
     1441 cccggcgcgg tctccgggat aatcgggggg ccgatgcgca tcgtcagggg gcggcgcagc
     1501 cagagctcat gtgtgccggt caggccgacg ggcagcagcg ggacatggtt gtgcagcgca
     1561 gcatgggccg cccccggatg cagcgactgg agccggcctt ccgcgtcacc gagccggccc
     1621 tccggaaaga tcagcaggcg gccaccgtgg gccaccatct cttccatagc ccgcgccgcg
     1681 cattgcggct gctggggatc gagggcaacc atgatttgca gccaatcgag gatcctgcgg
     1741 accagggggg ttgtctcgta gatcgcctcc atgcccagga catagatgcg cggttcggcg
     1801 gggaagaaca gcagaatcag gaagggatcg gcccagccga ggtgattggc gcagataatc
     1861 acgggggttg gggggatgtt gtccagcccg acgacgcgca cgcgaaacaa caggcggaaa
     1921 agtgttcgca ccagcaggcg caaggctttc tgatcccagc gggcgggcgc cgccgggccc
     1981 tgttggagcg gcacatgatg caccggtgcg gctgggtggg ccgcagacgt ggacgagttc
     2041 atcaaacccc ccttttcagc atgatgctgg aaagaacatc ggcctgacca ggatagactc
     2101 tcctaccccc cggcctcatg caagcgcacg cgttgcgcga ggcgcgccgg caggcgccat
     2161 tcacgccgat cctgcggcgc aggcggcggg gcggccgttt gcttcggcga tggtgactgt
     2221 tgcgtttgca tatgctggtc ctgttctcat ttgggaggcg gcgcccacat acagccccag
     2281 tatctattct ggatctgtaa ccgcaacatc ccggtagaaa atcgtaaaca gaccgtggca
     2341 aatcttgccc gctcgccggc cacggcttga cggggcagca agagccggct atagcgatga
     2401 acgaaccggt ttgttcgtca agcaggaagg agttattccg cggtctcaag gtggggatga
     2461 agattcggtc gaggtgcaac gggtccgcca cctcacgcca cgggctgtag gggcgcagtc
     2521 ggagggatgt cgtagtaata gacctgacca tcgcgaaagt agatcagggt gggctgcatg
     2581 tccgcttgct cacgatagta gcccacccag ccctcggtgg cgctgtactg cgccaaccag
     2641 acgaacatga acaaccagac ctggtagaaa tcgtcgtcca atgcctcgct gcggatggtc
     2701 agggtgtgct gcgcgttgtt gcggcgccag gacggggcag cagcgaggat gccgattgcc
     2761 gatccggctc cgtaccacag cgggtcccga ttttgcagca tctggcgcca ccagggatcg
     2821 ccggcgtcag gggcgtagcc gatgacgggt tctggaggcg ggccgtcgta cgcatagtct
     2881 tccgtgcgag tcaggtactg gagcagggcg ataatgtccg gtggggtatc cggtttgagg
     2941 tcaaaggcca gcataaattc gtagtgcaaa ctcatcgcac tgatccttgc cgatacatac
     3001 cttacctctc cccggcgcgc ggcagacggc gatgggcgac cagcagcgcg aggccactcc
     3061 cggcgcccgc gatgagcgcc gcgcccgccc cggcggccag ccagagccaa agcggcgccg
     3121 gggactcggt ctgggcggcc acgaccagcg gcaggcagag gatgccgccg gtgaggccgc
     3181 cgagcgccag ggccaggagt tcgccgagca agcgcgcgcc gccgtccagc agcgggccgg
     3241 cgggatcggg ccgggcgagg acgacgccca tgctgagggt gacggcgagc atgctcaggc
     3301 caatgagttc gacgccgccc cagccgtaga ggaagccgcc caggctgaac ccgcgccaga
     3361 ggcccacgac gccgagcaac gcgctgctga gcagcacgaa cggcagcgcc agggccagga
     3421 accagccgcg caagatagac gccccgccga tgggcgcgag gcgcaggatc gaccaggcgc
     3481 ggccctggcg gctcagagtg ctgaggctca gttcgaggaa cagcagcgcg gccatgacca
     3541 ggaccacggc ggccaggaag gcgttgattt gcattgcgag ttgcggcggg gcagcaccac
     3601 cgccgagggc cgca
//
LOCUS       DASUYC010000525         4944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1879262, whole genome shotgun sequence.
ACCESSION   DASUYC010000525 DASUYC010000000
VERSION     DASUYC010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4944
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1879262"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VF276_08635"
     CDS             complement(<1..339)
                     /locus_tag="VF276_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606964.1"
                     /translation="MTAFLIRRLFQGFLVLVVSTAIIYFLLTLIPGGPLTGLRAPTAR
                     ISKTDINRLGHMMGITDLQGHEYPWYTRYFNWLFSPEKNDVDVTIAGVHIQGGGILTG
                     DWGDSITLAQG"
     gene            585..2693
                     /locus_tag="VF276_08640"
     CDS             585..2693
                     /locus_tag="VF276_08640"
                     /inference="COORDINATES: protein motif:HMM:NF044618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606965.1"
                     /translation="MSQVPRRSSRRPPVPPVLLVLLGVAVLALLAVPLLARSPVSPAP
                     GPTATAQANTPPTAVAAPSETAPPPPDTASPASPAPPATAGGIDTPTAPPAVSPAAPS
                     APAASFSPQSTLAALASAQVPRRDLYLLTQQLKARTTEPLPRTTGSGPRNLQVGATEV
                     FNVGNNQDGSYYTVTATLRDITPHAYWWVSSDSDIDLDALRQSAQHFETKIYPTNVAA
                     FGAPPDPGVDGDSHINILNTALIGAAGYFSAADMYTRAVNPYSNERKMIYAGVAPGTT
                     SFEGLLAHEFQHMIHWKVHPNQNIWINEGMAELAMKINGYDTGGPESLFMSNPDVQLN
                     NWSSPPNTALPHYGAAYLFMNYLYDHYGPQIIHAIMAAPGADIDAIDEAFAALGKPER
                     FDTVFTNWALANWLDKTTTDPRYQYPHLQVEVEARATLAGAEANYNGSVDQQAADYVV
                     LDGAAGGITTVTFQGEQTVPLIPARPHSGQGLWWSNRDDVSAASLTHSFDLRAVSRAT
                     LSYWTWYAIEDGYDYAYAEISADGGQTWTTLRGAGTTSDNPNGNNLGQGYTGNSGGGE
                     TPAWIQEQIDLTPYAGKEVQVRFQYITDDGLNLQGFAVDDIRIPEIGFQDDAETPGGN
                     GWAAEGFVQVDPVLPQRFAVALIRYGADGAPSVEMVPLDGQNRGQVALKGAQRAVLMV
                     APLAPATTQPGRYQLTVTKP"
     gene            complement(2805..>4944)
                     /locus_tag="VF276_08645"
     CDS             complement(2805..>4944)
                     /locus_tag="VF276_08645"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HEX6606966.1"
                     /translation="VTTDANGQAVVGIPLPDNLTTWRLTALAVTRDTQVGQATNEVVS
                     TKPLLVRPRVPRFLMSGDRVSPAVIIENRSGCPFDADVSLTLAGADFAKDGPAATQRV
                     RLDGEKLVSWQITAGQSPTATLSFSVGGGTCNGQAVSGDSLQIQLPVKPLLTTEDVAT
                     SGEVEQNSTATENVFLPHGVDPSQGELTLQVAPSLAAGAALAVDYVKDEDFDSTEQIV
                     SRFLPLLRLSKAYATANRTTPYSAEVPGLVNRAVAALYRGQHYDGGWGWYAESASDPY
                     LTAYALEGLAAAQAAGYDVSQAVLDNGANYLDGWLNAEPGDARDSNLRLDTRAYALYV
                     LAATGHPNLSRARALAVRENSLALYGRAYLALAFQRLGAPDDADRLLTNLAGAAKQTT
                     TTAHWEESATRTPSAYLDMDTDARTTALVLQALLIRDKDDPLVPKGVRWLMENRRAGH
                     WLSTQETAAVLSTLAAYMTASGELAGSGAWSVTINGQPWSAPAGPDGQIELRKAISDL
                     LINQDNPVTITRPDNSGRLYYNLNLRYTRAGAQVTARNEGLSILREYVRPGSGDGQGS
                     DPAAPVTSVAAGDLVEVRLTVIAPTDAYYLTAEDPLPAGLEAVNGTLRTTGLTERLDT
                     RPVATGNAKDGEPRQAETASFFDNVEMRDDRTLLFASYLPAGVYEYRYLARATTPGDY
                     SVLPASARLTYLPDVWGRSDSGHLTVTEAK"
BASE COUNT          770 a         1670 c         1723 g          781 t
ORIGIN      
        1 cccctgcgcg agggtaatgg aatcgcccca gtctccggtc agaatgccgc cgccctggat
       61 gtggacgccg gcgatggtca cgtccacgtc gttcttctcc ggcgagaaga gccagttgaa
      121 gtagcgggtg taccagggat attcatgccc ttgcaggtcg gtgatgccca tcatgtgccc
      181 tagccggttg atatccgttt tggaaatacg cgcagtcggc gcgcgcagcc cggtcagtgg
      241 gccgcccgga atcaacgtca gcaggaaata gatgatggcg gtcgatacga ccaggacgag
      301 gaagccctgg aacagtcgcc ggatcaaaaa cgcggtcatc gtattctcct cgaaccgact
      361 aagaggtatg aaacgccgtt acgccaagct aactgtggtt atcaccaggc gggaacttaa
      421 ccgccagtgt accaaagccg gcccgtcatg tcaagcgtga tcacaagata gtaccgcccg
      481 cgccggggcg cggttcaaat agtgatcagc aatggcacga tacggtgtaa tagggtatag
      541 tgggacgatc gaaatgccgt cttaacgaga aagtgatcct gtttatgtcc caagtccccc
      601 gtcgttcgtc acgccgcccc ccggtgccgc cggtgctgct ggtgctgctg ggcgtggccg
      661 tgctcgccct gctggccgtg ccgctgctgg cccgttcgcc cgtgtcgccg gcccccggtc
      721 cgaccgccac agcccaggcg aacacaccgc ccacggccgt cgccgccccc tcggaaaccg
      781 ctccgccgcc gcctgacacc gcgagcccgg cctcgcctgc gccgccggcc acggcgggcg
      841 ggatcgatac tcccaccgct ccgcccgcgg tttcacccgc ggcgccttcg gccccggcgg
      901 cctcgttctc gccgcagagc accctggcgg ccctggcgag cgcgcaggtg ccccggcgcg
      961 acctctacct gttgacgcag caactgaagg cccggacgac ggagccgctg ccgcgcacca
     1021 cgggcagcgg gccgcgcaat ctccaggtgg gcgcgaccga ggtgttcaac gtcggcaaca
     1081 atcaggacgg ctcgtactat acggtcacgg cgacgttgcg cgacatcacg ccccatgcct
     1141 actggtgggt gtccagcgac tcggatattg acctcgacgc tctgcgccag tcggcccagc
     1201 acttcgagac gaagatctac cctaccaacg tcgcggcctt cggcgcaccg cccgaccccg
     1261 gcgtggacgg cgactcgcac atcaatatcc tcaacacggc gttgatcggg gccgcgggct
     1321 acttctccgc cgccgacatg tacacgcggg cggtgaaccc gtacagcaac gagcgcaaga
     1381 tgatctatgc cggggtggcg ccgggcacca ccagctttga agggctgctg gcgcacgaat
     1441 tccagcatat gatccactgg aaggtacatc ccaaccagaa tatctggatc aacgagggca
     1501 tggccgagct ggcgatgaag atcaacggct acgatacggg cggccccgag agcctgttca
     1561 tgagcaaccc cgacgtgcaa ctcaacaact ggtccagccc gcccaacacg gcgctcccgc
     1621 attacggcgc ggcctacctg ttcatgaact atctctacga ccactacggc ccgcagatca
     1681 tccacgccat catggccgcg cccggcgccg acatcgacgc catcgacgag gcgttcgccg
     1741 cgctaggcaa gccggagcgc ttcgacaccg tgttcaccaa ctgggcgctg gcgaactggc
     1801 ttgacaagac cacgaccgac ccgcgctacc agtaccctca cctgcaagtc gaggtcgagg
     1861 cgcgggccac gctcgccggc gccgaagcca actacaacgg cagcgtggac cagcaggcgg
     1921 cggattatgt ggtcctcgac ggcgccgcgg gggggatcac gaccgtgact ttccagggcg
     1981 agcagacggt gccgctgatc ccggcccggc cccactcggg ccagggcctg tggtggagca
     2041 accgcgacga cgtgtcggcg gccagcctga cgcacagctt cgacctgcgc gccgtgtcgc
     2101 gggcgacgct gtcttactgg acgtggtacg ccatcgagga cggctatgac tatgcctacg
     2161 ctgagatttc agccgacggc gggcagacct ggacgaccct gcggggcgcc ggcaccacca
     2221 gcgacaaccc caacggcaac aatctgggcc agggctacac cggcaacagc ggcggcggcg
     2281 agacgcccgc ctggatccag gagcagatcg acctgacgcc ctacgccggg aaggaggtgc
     2341 aggtgcgctt ccagtacatc accgacgacg gcctcaacct gcaaggcttc gcggtggacg
     2401 acatccgcat cccggagatc ggtttccagg acgacgccga gacgccgggc gggaacggct
     2461 gggcggcgga gggctttgtc caggtcgatc cggtgctgcc gcagcgcttc gccgtcgcgc
     2521 tgatccgcta cggcgcggat ggcgcgccca gcgtggagat ggtgccgctg gacggccaga
     2581 atcgggggca ggtggcgctg aagggcgcgc agcgggccgt gctgatggtg gcgccgctgg
     2641 ctcccgcgac cacccagccg gggcggtatc aactcacggt gacgaagccc tgatggacgg
     2701 cgggccggtt tcaaaccggc ccctacaacg caccctcacc atgcgctcag ggggataggc
     2761 cgggggcgtg cccatcttac agggcgcgcc cccggcttgt ttgcttactt ggcctcggtg
     2821 acggtcaggt ggccgctgtc gctgcggccc cacacgtcgg gcagataggt caggcgggcg
     2881 ctggcgggca ggaccgagta gtcgccgggc gtggtggccc gcgccaggta acgatactcg
     2941 tagacccccg ccggcagata cgacgcgaag agcagcgtgc ggtcgtcgcg catctcgacg
     3001 ttgtcgaaga acgacgcggt ttccgcctgc cggggttcgc cgtctttggc gttgccggtc
     3061 gccaccgggc gggtgtcgag ccgctcggtg aggccggtgg tgcgcagggt gccgttgacg
     3121 gcctccagcc cggcgggcag cgggtcttcc gccgtcaggt agtaggcgtc ggtgggcgcg
     3181 atgaccgtca gccggacctc caccaggtcg cccgcggcca cactggtcac cggggcggcg
     3241 ggatcgctcc cctggccgtc gccggacccc ggccgcacgt attcccgcag gatgctcagg
     3301 ccctcgttgc gcgcggtgac ttgcgccccc gcccgcgtgt agcgcaggtt caggttgtag
     3361 tacagtcgcc cgctattgtc gggccgggtg atggtgaccg ggttgtcctg gttgatcagc
     3421 aggtcgctga tcgccttgcg cagttcgatc tggccgtccg gcccggcggg tgcgctccac
     3481 ggctgaccgt tgatggtgac gctccaggcg ccgctgccgg ccagttcgcc ggaggcggtc
     3541 atgtaggcgg ccagcgtgct gagcaccgcc gccgtctcct gggtgctgag ccagtggccg
     3601 gcgcgccggt tctccatcag ccagcgcacg cccttgggca ccagcgggtc gtctttgtcg
     3661 cggatcagca gcgcttgcag caccagggcg gtcgtgcgcg catcggtgtc catgtcgaga
     3721 taggccgacg gcgtgcgcgt tgcgctttcc tcccagtgcg cggtggtggt cgtctgcttg
     3781 gccgcgccgg ccaggttggt gagcaggcgg tcggcgtcgt cgggcgcgcc gagccgctgg
     3841 aaggccaggg ccaggtaggc gcggccatag agggccaggc tgttctcgcg cacggccagg
     3901 gcgcgggcgc gggacaggtt cgggtggccg gtggcggcca gcacgtagag cgcgtaggcg
     3961 cgggtgtcga ggcgcaggtt ggagtcgcgg gcgtcgcccg gctcggcgtt gagccagccg
     4021 tccaggtagt tggcgccgtt gtcgagcacg gcctggctca cgtcgtagcc cgcggcctgg
     4081 gcggcggcca gcccttccag cgcatacgcc gtcaggtagg ggtcgctggc cgactcggcg
     4141 taccagcccc agccgccgtc gtagtgctgg ccgcgataga gcgccgccac cgcccggttg
     4201 accagacccg gcacctcggc gctgtacggg gtggtgcggt tggccgttgc gtaggctttg
     4261 ctgagccgca gcagcggcag gaagcggctg acgatctgct cggtgctgtc gaagtcctca
     4321 tccttgacat aatcaacagc cagcgcggcc cccgcggcca gcgacggagc gacttgcagg
     4381 gtcagttcgc cctggctcgg atcgacgccg tgcggcagga agacgttctc ggtggccgtg
     4441 ctgttctgct cgacctcgcc gctggtggcc acgtcctcgg tggtgagcag cggcttgacc
     4501 ggcagttgga tctgcagcga gtcgccgctc accgcctgcc cgttgcaggt gccgccgccc
     4561 acgctgaagc taagcgtggc ggtggggctt tgcccggcgg tgatctgcca ggagaccagc
     4621 ttctcgccgt ccagccggac gcgctgggtg gcggcggggc cgtccttggc aaaatcggcg
     4681 ccggccaggg tcaggctcac gtcggcgtcg aaggggcagc cgctgcggtt ctcgatgatc
     4741 acggcgggcg acacgcggtc gccgctcatc aggaagcggg gcacgcgcgg gcgcaccaac
     4801 agcggcttgg tggagacgac ctcattggtc gcctggccca cttgcgtgtc gcgggtcacg
     4861 gccagcgcgg tgaggcgcca ggtggtcagg ttgtcgggca gggggatgcc gacgacggcc
     4921 tgaccgttgg cgtcggtcgt cacc
//
LOCUS       DASUYC010000526         2483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1643283, whole genome shotgun sequence.
ACCESSION   DASUYC010000526 DASUYC010000000
VERSION     DASUYC010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1643283"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VF276_08650"
     CDS             complement(<1..243)
                     /locus_tag="VF276_08650"
                     /inference="COORDINATES: protein motif:HMM:NF024298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX6606967.1"
                     /translation="MQKRYILRTVNLCLLLMLCACDLASPTANTPNLAAQLKDSAGWI
                     NSLSWSPDSTLLAVGSRDGGVRIWDVADHRIHLRLPD"
     gene            complement(388..933)
                     /locus_tag="VF276_08655"
     CDS             complement(388..933)
                     /locus_tag="VF276_08655"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015114932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003950 - NAD+ ADP-ribosyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HEX6606968.1"
                     /translation="MDDARLVKVSKYLSKHLRHQPQRLGLTLQPGGWVPVEDLLAACA
                     RMHFPISRAELEEVIARNNKQRFALDATGTLIRANQGHSVPVDLQLAQVAPPPMLYHG
                     TAAHLVETIQREGLRKMARHHVHLSPDPETARIVGARHGSPVVFAVDAATMQQAGTPF
                     YCSANGVWLVDAVPPAYLRLL"
     gene            1158..2084
                     /locus_tag="VF276_08660"
     CDS             1158..2084
                     /locus_tag="VF276_08660"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEX6606969.1"
                     /translation="MPDLTILQTIPLFRELPDAELEALGMLLVEREFPQGSDIVRVDE
                     PGDTFYVIQEGEVDLSLRDAEGRYVPLDRIDAGEFFGELAMLTGEARSATATAVTPVR
                     VWELDRDTFYRFLTAYPTSAIRVLIVLARRLRDTEHLLEVQVSENPNRQGDDHLTAVQ
                     RIADAIADFSGSLPFLALNAGIFAAWIVVNLPGSPIAFDPFPFGFLTMSVSLEAIFLS
                     IFVLISQNRQAAKDRIKSEGDYRVNLKAELEIGLVLKNLREIQDRIEVMQRDQARFHS
                     LVAHNGHGSGPLVASAIAPDAGTPQMEAADQG"
     gene            complement(2133..>2483)
                     /locus_tag="VF276_08665"
     CDS             complement(2133..>2483)
                     /locus_tag="VF276_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606970.1"
                     /translation="DDWHAQGTYLPSHNRYLVGQVGKPAPTATPDPSVLPTVDPALTP
                     GAPPVVPGTDPAAQPPAPGTTPAPPAAQPPANPPAPDPAAPPPAAPQAPTPVPQAPAP
                     EQAPPAATPAPANP"
BASE COUNT          419 a          764 c          848 g          452 t
ORIGIN      
        1 atcgggcaag cgtaagtgga tacgatgatc ggccacatcc caaatacgca ctcccccgtc
       61 acgcgatccc accgcaagga gagtgctgtc tggtgaccaa gatagactgt taatccatcc
      121 cgcggaatct ttcaactgtg cagccaggtt cggtgtattt gcagttggac ttgccaaatc
      181 acaagcacag agcatcaaca aaaggcaaag attaacggtg cgaaggatat agcgtttctg
      241 cataactatc cttagtagaa ctgccttttg tatgcgcggc gtattattgt ctgcccgcag
      301 accttcgctt ccgttagcat gacacacagc cttgcggacc acgccgatac tcttcgctcc
      361 gcttagagtg acatacggaa agacggctta tagtaaccgc aaatacgccg ggggcacagc
      421 atcgaccagc cagacgccgt tcgccgagca gtagaacggg gtgcccgcct gctgcatcgt
      481 ggctgcgtcc actgcgaaga cgaccggcga cccgtggcgg gcgccgacga tgcgcgccgt
      541 ctccgggtcg ggcgacaggt ggacgtggtg gcgcgccatc ttccgcaagc cctcgcgctg
      601 gatggtttcg accaggtggg ccgccgtgcc gtggtagagc atcgggggcg gcgcgacctg
      661 cgccagttgc agatcgaccg gcacgctgtg gccctggtta gcgcggatca gcgtgccggt
      721 ggcgtcgagc gcgaagcgct gcttgttgtt gcgggcgatc acttcctcca gttcggcgcg
      781 gctgatgggg aagtgcatgc gggcgcaggc ggcgagcaga tcctcgaccg gcacccagcc
      841 gccgggctgc aaggtcaagc ccaggcgctg cggctggtgg cgcaggtgtt tgctcagata
      901 tttgctgacc ttgaccagcc gtgcgtcgtc cattggtttc ccctcctaat cgcccgcgtc
      961 caatcatgcc gggagcggcg tgggaatagc tgatagcaat ggtacgcggt cctatggcaa
     1021 acagatgtgc ggggccgggg tgggcgggac ggatcggcac atagttgatg ccgtcgccgg
     1081 ctcgtgctac actgatgcct gtagtgtgcc gtcccgccgt agcggcccgg cgcggatggc
     1141 cggcgaggac ctccgcgatg cctgacctga ccatcctgca aacgattccc ctctttcgcg
     1201 aactccccga tgccgaactg gaagcgctgg gcatgctgct ggtcgagcgc gagtttccgc
     1261 aaggcagcga tattgtccgc gttgacgagc cgggcgatac gttttatgtg atccaagagg
     1321 gcgaggtcga cctgtcgttg agagacgccg aaggtcgcta cgtgcccctg gatcgtatcg
     1381 acgcgggcga gtttttcggc gaactggcga tgctcaccgg cgaagcccgt tcggccaccg
     1441 ccaccgcggt gacaccggtg cgggtgtggg aactcgaccg ggacactttc tatcggtttc
     1501 tcaccgcgta ccccaccagc gcgatccgag tgctgatcgt gctggcgcgc cgcctgcgcg
     1561 ataccgaaca cttgttggag gttcaggtca gcgaaaaccc gaatcgccag ggcgacgacc
     1621 acctcacggc ggtccagcgc atcgccgatg ctatcgccga tttcagcggc agcctgccgt
     1681 tcctggcgct taatgccggg atcttcgccg cctggatcgt cgtgaacctg ccgggcagtc
     1741 ccattgcgtt cgatcccttc cccttcggct ttctgacgat gagtgtgtcg ttggaagcga
     1801 tcttcttgtc gatctttgtt ctcattagcc agaaccggca agcggccaaa gaccgcatca
     1861 agtccgaagg cgattaccgg gtgaacctga aagccgagtt ggagatcggg ctcgtgctca
     1921 aaaatctgcg tgagatacag gaccgtatcg aagtgatgca gcgcgaccag gcacgcttcc
     1981 acagcctggt cgcccataac ggccacggga gcggaccgct ggttgcgtcc gcgatcgcgc
     2041 cggacgcggg cacgccgcaa atggaggcgg cagaccaggg ctaacgtgcc tgtggtctgc
     2101 cgccttcggg tggccggtat gctaccgccc gatcagggat tggccggcgc cggggttgcg
     2161 gcgggcggcg cctgctcggg cgcgggcgcc tggggcaccg gagtcggcgc ctgcggagcc
     2221 gcgggcggcg gggctgccgg gtccggtgcc ggggggttgg cgggcggctg ggccgcgggc
     2281 ggggccgggg tcgtgccggg cgcgggcggc tgagccgccg gatcggtgcc gggcaccacc
     2341 ggcggcgcgc cgggcgtgag cgccgggtcg acggtcggca gcacggacgg atccggagtc
     2401 gccgtcggcg cgggcttgcc cacctggccc accaggtaac gattgtgcga cggcagatag
     2461 gtgccctggg catgccaatc gtc
//
LOCUS       DASUYC010000527         3688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1503541, whole genome shotgun sequence.
ACCESSION   DASUYC010000527 DASUYC010000000
VERSION     DASUYC010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1503541"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..828)
                     /locus_tag="VF276_08670"
     CDS             complement(163..828)
                     /locus_tag="VF276_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011140820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6606971.1"
                     /translation="MTDQPNPGRYAGTDRPEHWEELYQQGNTGWDLGGPAPAFIDLLA
                     GPDAPPPGRMLVLGSGRGHDALLFAAQGFDVRGVDFAPSAVAAATEAAAARGLSERAH
                     FEQRDIFALPPSYREAFDYVLEHTCFAAIDPRLNEEYAALVPRLLRPGGRYLALFFAH
                     GRPGGPPYATTLDAVRRRFAPRLEILRLETAQHSVPSRAGRELFAVMRKPPGPIDGPP
                     EVQ"
     gene            complement(1083..1697)
                     /gene="coaE"
                     /locus_tag="VF276_08675"
     CDS             complement(1083..1697)
                     /gene="coaE"
                     /locus_tag="VF276_08675"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122632.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX6606972.1"
                     /translation="MGEASQLGDKFIIGLTGNIACGKSTVLARLAALGAGVIDADVVT
                     RELQRRGAPAYGPMVEAFGPDIVQPDGEIDRRALGNRVFADPVALRRLEGLVRPLVRD
                     EIVRRIAALPQRVVVVDAIGLLDGPLVDYCRRVWVVTCLPEQQVARLVATRGLTEEEA
                     RLRVNAQAPQAAKVARADLVFDNSGTREALLAQVDAAWAALPTA"
     gene            complement(1676..>3688)
                     /locus_tag="VF276_08680"
     CDS             complement(1676..>3688)
                     /locus_tag="VF276_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX6606973.1"
                     /translation="LTKTFHLEPIFEGVSFQLNEGEKVALVGANGAGKTTILEIIAGL
                     EEPDTGGGTVVRARGTRLAYLPQEVAGFGSLLGGPAPAPDATLWDLMLDTLGEVRDLQ
                     REMNRLEEAMSAPGAPQTGPAWDRLIAVYEAVTERFEQAGGYDLEHRIEQVLEGLGFK
                     PRQFDTPLAQLSGGQKTRAALARALLSDPDVLLLDEPTNHLDLSAIEWLEGFLQTWRG
                     TLLCASHDRRFLDKVTTRTLDLEFGTLESYPGNYSKYLLLKAERMERRLAEYEAQQEQ
                     IAKTEEFIRRFKAGQRSKEAKGREKRLKRLERLHAPRSRETLKLELQAHLRSGRTVMA
                     TEDLVVGYCGVVPDPAGGPPGDLVLARCPDLEIERGEHVALIGANGTGKTSLLRTAIG
                     ELPPLHGQIDLGHNVEFAYYAQAHEGLDPTNRVIDEILYTLPMTEESARNLLARFLFT
                     GDDVYKRVGDLSGGERSRVALAKLTLLPANFLLLDEPTNHLDLGAREALEDVLSEYQG
                     TLLFVSHDRAFIDSLATQTWVLDNAVIEAFDGNYSDYQEELARRRREAVGGEPAPAGP
                     RKPGAPGARPGKGAPAALTPREEQRRREEAERAAQRAKAAAARKLQEVEQTIGALEAR
                     LNLLSAELDAASAASDIALVTDLGLEYQQLAEELEQKYGEWEKLAS"
BASE COUNT          523 a         1268 c         1299 g          598 t
ORIGIN      
        1 gcccgcccca gacgcggcta tggaactcgg gctgcaacag gaagggatac ggctcgggca
       61 cgccgtgcgc gggatggccg gcagggttgg acgttgtcat cgcgtgtgta cctcctcaga
      121 gcggctagcg gcatcgaatc ccggcggcgt cacgccgggt gcttattgca cttcgggcgg
      181 cccgtcgatg gggccgggcg gcttgcgcat cacggcgaac agctcgcgcc ccgcccgtga
      241 cggcaccgaa tgctgggcgg tctccagccg caggatctcc aggcgtggcg cgaaccggcg
      301 gcgcaccgca tccagcgtgg tggcatacgg cgggccaccg gggcgcccgt gcgcgaagaa
      361 cagcgccagg tagcgaccgc cggggcgcag caggcgcggc accagcgcgg cgtattcctc
      421 gttcagccgg gggtcgatgg cggcgaagca ggtatgttcc agaacgtagt cgaaagcctc
      481 gcggtaggac ggcggcaggg caaatatatc gcgttgctcg aagtgggcgc gctcgctcag
      541 cccgcgcgcc gctgccgcct cggtcgcggc ggccaccgcc gagggcgcga agtcgacgcc
      601 ccgcacatcg aagccctgcg cggcgaagag cagcgcgtca tgcccgcgcc cgctgccgag
      661 gacgagcatc cgccccggcg gcggcgcgtc cggcccggcc agcaggtcga tgaaggcggg
      721 cgccgggccg cccagatccc agccggtgtt gccctgctgg tatagctcct cccagtgttc
      781 gggccggtcc gtgcctgcgt agcgccctgg attgggttgg tcggtcatta tgcctccagt
      841 gaaaatgaag catgacggct gcgataaacg atcctctgtt gtgtagccgc gcgatttatc
      901 gcgccgtact cggttatcgg cagcgtgctg gtcgcgcaac cgagcctctt cgcgacgatc
      961 agagcgatag gcgtccgtag tccgccggct tgattctgat gggccgcagg ccggtttgaa
     1021 accggcccct acagcgcaaa attaccgtgg tcgcctacca gccccggcgc tgatggctgg
     1081 tctcacgcgg tgggcagcgc ggcccaggcg gcgtctacct gggccagcag ggcctcgcgg
     1141 gtgccgctgt tgtcgaagac gaggtcggcg cgggcgactt tggccgcttg cggggcctgg
     1201 gcgttgacac gcagccgggc ttcctcctcg gtgaggccgc gggtggcgac caggcgggcc
     1261 acctgctgct ccggcaggca ggtcacgacc cagacgcggc ggcagtagtc gaccagcggg
     1321 ccgtcgagca ggccgatggc atcgaccacg accacgcgct gcggcagcgc ggcgatgcgg
     1381 cgcacgatct cgtcgcggac caggggccgg accaggccct ccaggcggcg cagggcgacg
     1441 gggtcggcga agacccggtt gcccagggcg cggcggtcga tctcgccgtc gggttgcacg
     1501 atgtccgggc cgaacgcctc gaccatgggg ccgtaggccg gggcgccgcg ccgctgcaac
     1561 tcgcgcgtga ccacgtcggc gtcgatcacc ccggcgccga gggcggccag gcgggccagt
     1621 accgtgcttt tgccgcaggc gatgttgccg gtcagcccga tgatgaactt gtcgcctagc
     1681 tggctagctt ctcccattcg ccgtacttct gctccaactc ctcggccaac tgctgatact
     1741 ccaggcccag gtccgtaacc agggcgatgt cggacgctgc gctggcagcg tcgagttcgg
     1801 cgctgagcag attgagccgg gcttccagcg cgccgatggt ctgctccacc tcctgcaatt
     1861 tgcgggcggc ggcggctttg gcccgctggg cggcgcgctc agcttcctcg cgacggcgct
     1921 gctcctcgcg cggggtgagc gcggcgggag cgcctttacc aggccgtgcc cctggtgccc
     1981 ccggtttgcg tggcccagcg ggcgcgggct cgccgcccac ggcctcgcgg cggcggcggg
     2041 ccagctcttc ctggtaatcg ctgtagttgc cgtcgaacgc ctcgatcacc gcgttgtcga
     2101 gcacccaggt ctgcgtcgcc aggctgtcga tgaaggcgcg gtcgtgcgag acgaacagca
     2161 gcgtgccctg gtactcgctc agcacgtctt ccagcgcctc gcgggcgccc aggtcgaggt
     2221 ggttcgtcgg ttcgtccagc agcaggaagt tggcgggcaa gagagtcagc ttcgccaggg
     2281 ccacccggct gcgctcgccg ccgctgaggt cgccgacgcg cttgtacacg tcgtcacccg
     2341 tgaacaggaa acgcgccagc aggttgcggg ccgactcctc ggtcatgggc agggtataga
     2401 ggatctcgtc gatcacccgg ttggtcgggt ccagcccttc gtgggcctgc gcgtaatagg
     2461 cgaactcgac attgtggccc aggtcgatct ggccgtgcaa cggcggcagt tcgccgatag
     2521 ccgtacgcag cagcgaggtc ttgcccgtgc cgttggcgcc gatgagcgcc acgtgctcgc
     2581 cccgctcgat ctccaggtcg gggcagcggg ccagcaccag atcgcccggc ggccccccgg
     2641 cgggatcggg caccacgccg caatagccca ccaccaggtc ctcggtcgcc atgacggtgc
     2701 gcccgctgcg caggtgggcc tgtagctcca gcttgagcgt ttcgcggctg cgcggcgcat
     2761 gcaaccgttc caggcgcttg aggcgctttt cgcggccctt ggcctccttc gagcgctgcc
     2821 cggccttgaa gcggcggata aactcctcgg tcttggcgat ctgctcctgc tgggcttcgt
     2881 actcggccag gcggcgctcc atgcgctcgg ccttcagcag cagatatttg ctgtagttgc
     2941 cgggatacga ttccagcgtg ccgaactcca ggtcgagcgt gcgcgtggtc actttgtcga
     3001 ggaaacggcg gtcgtgcgag gcgcagagca acgtgccccg ccaggtctgc aagaagcctt
     3061 cgagccattc gatagcggac aggtcgaggt ggttggtcgg ctcgtccagc agcaacacat
     3121 cgggatcgga cagcagcgca cgggccaggg ccgcgcgggt cttctgcccg ccgctcagtt
     3181 gggccagcgg cgtatcgaac tggcgcggct tgaagcccag gccctccagc acctgctcga
     3241 tacggtgttc caggtcgtag ccgcccgcct gctcgaagcg ctcggtcacg gcctcgtaca
     3301 cggcgatcag ccggtcccag gccgggccgg tctgcggcgc gccgggggcc gacatggcct
     3361 cttccagccg gttcatctcg cgttgcagat cgcgcacttc gcccagcgta tcgagcatca
     3421 ggtcccagag cgtggcatcg ggggcgggcg cggggccgcc cagcagcgag ccgaacccgg
     3481 ccacctcctg cggcaaatag gccaggcgcg tgccccgcgc ccggaccacg gtgccgccgc
     3541 ccgtatccgg ctcttccagc ccggcgataa tctccagaat cgtcgtctta cccgcgccgt
     3601 tggcaccgac cagcgcgacc ttctcgcctt cgttcagctg aaagctgacg ccctcgaaga
     3661 tcggctccag atggaaggtt tttgttaa
//
LOCUS       DASUYC010000528         2916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1412845, whole genome shotgun sequence.
ACCESSION   DASUYC010000528 DASUYC010000000
VERSION     DASUYC010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1412845"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(417..2006)
                     /locus_tag="VF276_08685"
     CDS             complement(417..2006)
                     /locus_tag="VF276_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606974.1"
                     /translation="MAQPPAAPDPVPRLPRIWPIGIFLVTLIVYAIFTNQVLIYTNPP
                     TGDQPFYLHTAISIVQDHDINEANNYADQDERLYYPRTVWEDGYYPAGFKGIPAPYPL
                     PPHNGHTTNRPATEQYSKHGLGLSVLIVPGWALGVNLDPASLGLAELEQPTAGWPVVV
                     YQLNILGALLAAQVFLLAYEATRRRGIALVVWLALAFSNPLMTYSYLIFPELPAGLCA
                     IYAFRRLRLGWATNGRLQLLLIGLALGFMPWLHARFLPITGALALYAIWAWRRARQDS
                     PAGRGTWPRLVMALAPIGISLVLLMNFYYWLYGTVLPNTGDHAGFINPLTDPLGLPVS
                     IVGLLIDQQWGLLIAAPVYLLAFVGMAALWMTPGQRATAGWLLFALIPYYLVVAEYRQ
                     WWGEWCPPARYLATIAPLLALPLARGLLALQRSRLYSILFGFLAALGWAYMAGLLAHP
                     RSFWNHPSGQSDLFLWVSKEFHLNLVPVFPAIVPWSWDHTAPIKWGLVIGWGAIAVVI
                     FLAGLLLLQESRQPSAVSRQG"
     gene            2204..>2916
                     /locus_tag="VF276_08690"
     CDS             2204..>2916
                     /locus_tag="VF276_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX6606975.1"
                     /translation="MAQICPACKTSNDDGATRCRQCGAALPGNAAKTTGGDGATSGKT
                     TYRPAGTTPGASAGSASGTPAGSTSGTPAGSSPAARRAASSGAIAEAARRAAAQQKAA
                     QAPPAAPATRAGTPGTRGEARTTPATRSPVRGPSRGIAAPINNALDRAGLPRFSTQRY
                     LVIWAIFAVILVAVIIALVAIGASNARPVVPTPQPGAVLTGTVATVETSQGVFKLKLN
                     TDDPSMKDTIENFRVKAASG"
BASE COUNT          516 a         1009 c          974 g          417 t
ORIGIN      
        1 caccaggtcg gggcggatgt cgcgcgccag cgtgatcgcc gtctccgcgt cggcggcctc
       61 gcccgccacg gtgtagccca tcgcggtcag cagatcgcgc aggtccatgc gaataatcga
      121 gtcgtcatcc acaatgatga ggcgggtttg tgccatggaa cgtttcctcc gctagatccc
      181 ggactcattt gcgcccggcc ccattgccgg actacgggct ggtattataa agcctgattc
      241 cagcgcctgt caagcgtgac gagccgtcag ccgtcagccg tcagccatca gccgtcagcc
      301 gtcaggtgtt agcagcctgt agcgcatcca tagcgaatct tgggccacga cccgatcagc
      361 gcactctcgc ctacctggcc cgtgacattc gtatcggctg cgcggagatg cgtatctcat
      421 ccctgacggc tgacggctga cggctgacgg ctctcctgca agagcagcaa cccggccagg
      481 aagatcacca cagcgatggc gccccagccg atgaccaggc cccacttgat gggcgccgta
      541 tggtcccacg accagggcac gatggcggga aagaccggca ccaggttgag gtggaactcc
      601 ttgctgaccc agaggaagag atcgctctgg cccgacgggt ggttccagaa cgagcgcggg
      661 tgggcgagca ggccggccat gtaggcccag ccgagtgcgg cgaggaagcc gaagaggatg
      721 ctatagaggc ggctgcgttg cagcgccagc agcccccggg ccagcggcag cgccagcagg
      781 ggcgcgatgg tcgccaggta gcgcgcggga gggcaccact cgccccacca ttgccggtac
      841 tcggccacca ccaggtaata cgggatcagc gcgaacagca gccaacccgc cgtggcgcgc
      901 tggccgggcg tcatccagag ggcggccatg ccgacgaagg ccagcaggta gaccggcgcc
      961 gcgatgagca gcccccactg ctggtcgatc aacagcccga ctatcgagac cggcaggccc
     1021 agcggatcgg tgagcgggtt gatgaagccc gcgtgatcgc cggtgttcgg caggacggtg
     1081 ccgtagagcc agtaatagaa gttcatcagc agcaccagcg aaatgccgat gggtgccagc
     1141 gccatgacta ggcgcggcca tgtgcccctg cccgccgggc tgtcctgccg ggcgcgccgc
     1201 cacgcccaga tcgcgtacag cgccagcgcg ccagtgatcg gcaggaagcg cgcgtgcaac
     1261 cagggcatga agccgagcgc cagcccgatc agcagcaatt gcagccgccc gttcgtggcc
     1321 cagccgagcc gcagacggcg gaaggcgtaa atcgcgcaca gtcccgccgg gagttcgggg
     1381 aagatcaggt agctgtaggt catcagcgga ttgctgaagg ccagcgccag ccagaccacc
     1441 agggcaatgc cccgccgccg cgtggcctcg taggccagca aaaacacctg cgccgccagc
     1501 agcgcgccca ggatgttgag ctggtagacg accaccggcc acccggcagt gggctgctcc
     1561 agttccgcca ggcccaggct cgccggatcg agattcactc ccagcgccca gcccggcaca
     1621 atcagcacgc tcaggcccag gccgtgcttg gagtattgct cggtggcggg ccggttggtg
     1681 gtgtggccgt tgtgcggcgg cagcgggtag ggcgccggga tgcccttgaa gcccgccgga
     1741 taatagccgt cctcccacac agtgcgcggg taatacagcc gctcgtcctg gtctgcgtag
     1801 ttgttggcct cgttgatgtc gtggtcctgc acgatgctga tcgccgtgtg caggtagaac
     1861 ggctggtcac cggtcggggg gttggtatag atgagcacct ggttggtgaa gatcgcgtag
     1921 acgatcaacg tgaccaggaa gatgccgatg ggccagatgc gcgggaggcg ggggacggga
     1981 tcgggcgctg cgggcggctg cgccacggag gcaggctcca caacgggatc gagcaatgac
     2041 atatgcgcct gggttcctct ccacgctgca aggctggccg cacttgtgcc gggctaaact
     2101 gccgtattat accataactt gacgctccgg cgcgcctgtg gtagaaatgc acggggggcg
     2161 atgcccgaca tcgcccgacc cgcagaaagg aagccgattt accatggcgc agatctgccc
     2221 tgcgtgcaaa accagtaacg acgacggcgc caccaggtgc cgccaatgtg gcgcggccct
     2281 gcccggcaac gcggccaaga ccacaggcgg cgacggggcg acctccggca aaacgaccta
     2341 ccggccggcg ggcaccacgc ccggcgcatc tgccggttcc gcctccggca cgcccgccgg
     2401 ttccacctcc ggcacgcccg ccggctcctc tccggcggcc cgccgcgcgg cctcgtccgg
     2461 cgctatcgcc gaagccgccc gccgcgctgc cgcccagcag aaggcggccc aggccccgcc
     2521 tgccgccccg gccacccgcg ccggcacgcc cggcactcgc ggcgaagccc gcaccacgcc
     2581 ggccacccgc agtcccgtcc gcggcccatc gcgcggcatc gccgcgccga ttaacaatgc
     2641 gctcgaccgg gccgggctgc cccgtttcag cacccagcgt tatctggtca tctgggcgat
     2701 cttcgccgtg atcctggtcg ccgtgattat cgcgctggtc gccatcggcg cctcgaacgc
     2761 gcgtccggtt gtgcccacgc cgcagcccgg cgccgtcttg accggcaccg tggcgacggt
     2821 cgagacctcg cagggcgtct ttaagctcaa gctgaacacc gacgacccgt cgatgaaaga
     2881 caccatcgag aacttccggg tgaaggccgc cagcgg
//
LOCUS       DASUYC010000529         2270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1887550, whole genome shotgun sequence.
ACCESSION   DASUYC010000529 DASUYC010000000
VERSION     DASUYC010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1887550"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VF276_08695"
     CDS             complement(<1..497)
                     /locus_tag="VF276_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX6606976.1"
                     /translation="MSAVLPPVSGSGKRPSSPILPSPAAANAAAASFAALTARLDYLE
                     PAEVDLVRKAYRFADEAHLGQIRASGEPYITHPIAVAAQCAEWKLDAQALMAALLHDA
                     IEDCGVTKPELIERFGAPVAELVDGLTKLDKLQFNTREENQAESFRKMLLAMARDVRV
                     ILVKLA"
     gene            complement(585..788)
                     /gene="rpoZ"
                     /locus_tag="VF276_08700"
     CDS             complement(585..788)
                     /gene="rpoZ"
                     /locus_tag="VF276_08700"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX6606977.1"
                     /translation="MARITVEDCLEKIPNRFQLVLAATYRARMLSQGHAPKIESKNKP
                     GVTALREIADGKIGLEMLKKVPG"
     gene            complement(810..1427)
                     /gene="gmk"
                     /locus_tag="VF276_08705"
     CDS             complement(810..1427)
                     /gene="gmk"
                     /locus_tag="VF276_08705"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX6606978.1"
                     /translation="MDYPGNLFVVAAPSGAGKSSLVKALLELDSHVQPSVSHTTRAPR
                     GQEKHGREYFFVSPGEFDQMVLADAFVEWANVHGQRYGTSKKAIEDRITHGADVILEI
                     DWQGALQIKRIFTNAVLIFILPPSWEELRSRLERRGEDAPDVIETRLANAAAELAQAK
                     EFDFVIINELFERALFDLKAIVHSQRLKYLSQRRLRADTFQALGI"
     gene            complement(1441..>2270)
                     /locus_tag="VF276_08710"
     CDS             complement(1441..>2270)
                     /locus_tag="VF276_08710"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017407182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEX6606979.1"
                     /translation="ARLGLEIRSVNSRFLDLSFRLGEELRAHEPALRELISGRIKRGK
                     VEVRAGVEGSGGDAVREPSMATLQRLAAVQDTVKTWMPTAAALSVAEILRLSSAEGDS
                     GVDWGEGLLQLAGQALGELVAAREREGARLAKMLQGHLQQLRALAQQARPLVPQLVEQ
                     QRQRFLERWKEAMALSDGAATPEAAQDRALTEATAFAIRIDVAEELTRLAAHLDEIER
                     LVAKGGEVGKRLDFLIQELHREANTLGSKSAALELTKISVDMKVLIEQMREQVQNIE"
BASE COUNT          316 a          750 c          803 g          401 t
ORIGIN      
        1 gccagcttca ccaggatcac gcgcacgtcg cgcgccatgg ccagcagcat cttgcggaag
       61 ctctcggcct ggttctcctc gcgggtgttg aactgcagct tgtccagctt ggtgaggccg
      121 tccaccagtt cggccaccgg cgcgccgaag cgctcgatca gctcgggctt ggtgacgccg
      181 caatcctcga tggcatcgtg cagcagggcc gccatgagcg cctgcgcgtc gagcttccac
      241 tcggcgcact gcgcggcgac ggcgatcggg tgggtgatgt agggctcgcc gctggcgcgg
      301 atctgcccca ggtgggcctc gtcggcgaag cggtaggcct tgcggaccag gtccacttcg
      361 gccggctcca ggtagtccag gcgggcggtc agcgcggcaa agctggccgc ggccgcattg
      421 gcggcggcgg gactggggag gatgggactg ctgggccgct tgccgctgcc cgacaccggg
      481 gggagcaccg cgctcatgcc cttaatgtag cgcgcggccc acggaagcgg gcagggccat
      541 gaaaaaacgc ccgcgagggg cgtttcttct ggaagtcgtt gggcttaacc ggggactttc
      601 ttcagcatct ccaggccgat cttgccgtcg gcgatctcgc gcagcgcggt cacgcccggc
      661 ttgttcttgc tttcgatctt gggcgcgtgg ccctggctca gcatgcgggc gcggtaggtc
      721 gcggccagca ccagctggaa gcggttcgga atcttctcga ggcagtcttc gacggtgata
      781 cgggccatga aatctctccg ggggtgggtt cagattccga gcgcctggaa ggtgtcggcg
      841 cgcaggcggc gctgggacag gtacttcagc cgctgggaat gcacgatcgc tttgaggtcg
      901 aaaagcgcgc gctcgaataa ctcgttgatt ataacgaaat cgaattcctt cgcctgcgcc
      961 agttcggcgg ccgcattggc gagacgggtt tcgatcacgt ccggcgcgtc ctcgccgcgc
     1021 cgctccaggc gcgagcgcag ttcctcccag ctggggggca ggatgaagat caggacggcg
     1081 ttggtgaaga tgcgcttgat ctgcagggcg ccctgccagt cgatctccag gatcacgtcg
     1141 gcgccgtgcg tgatgcggtc ctcgatcgcc ttcttggagg tgccgtagcg ctggccgtgg
     1201 acgttcgccc attcgacgaa ggcgtcggcc agcaccatct ggtcgaactc gccgggggag
     1261 acaaagaagt attcgcgccc gtgcttttcc tggccgcggg gggcccgggt ggtgtgcgac
     1321 accgagggct gcacgtggga atcgagctcc agcaaggcct tcaccaggct ggacttgccg
     1381 gcgccgctgg gggccgccac gacgaagagg ttgccggggt agtccatgcc ggattatcgg
     1441 ctactcgatg ttctggacct gctcgcgcat ttgttcgatg agaactttca tgtcgaccga
     1501 gatcttcgtc agctccagcg cggcggactt cgagccgagc gtgttggcct cccggtgcag
     1561 ttcctggatc aggaagtcca gccgcttgcc gacctcgccg cccttggcga ccaggcgctc
     1621 gatctcgtcc aggtgggcgg ccaggcgggt cagttcctcg gcgacgtcga tgcggatggc
     1681 gaaggcggtg gcctcggtca gggcccggtc ctgggcggcc tcgggcgtgg ccgcgccgtc
     1741 cgacagcgcc atggcctcct tccagcgttc caggaagcgc tggcgctgct gctccaccag
     1801 ttgcggcacc agcggccggg cctgttgcgc cagcgcccgc agctgctgca ggtggccctg
     1861 gagcatcttt gccaggcggg cgccttcgcg ctcgcgggcc gcgaccagct cgcccagggc
     1921 ctggccggcc aactgcagca ggccctcgcc ccagtccacg ccgctgtctc cttcggccga
     1981 cgacagccgc aggatctcgg cgacggacag ggcggccgcc gtgggcatcc aggtcttgac
     2041 cgtgtcctgc acggcggcca ggcgctgcag cgtggccatc gagggttcgc gcacggcgtc
     2101 gccgccgctg ccttcgaccc cggcgcgcac ttccaccttg ccccgcttga tccgtcccga
     2161 gatcagctcc cgcagggcgg gttcgtgggc gcgcagctcc tctcccagcc ggaaggacag
     2221 gtccaggaag cggctgttga ccgagcggat ttccagcccg agccgggcgc
//
LOCUS       DASUYC010000530         2056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1897906, whole genome shotgun sequence.
ACCESSION   DASUYC010000530 DASUYC010000000
VERSION     DASUYC010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1897906"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1615
                     /locus_tag="VF276_08715"
     CDS             <1..1615
                     /locus_tag="VF276_08715"
                     /inference="COORDINATES: protein motif:HMM:NF021570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HEX6606980.1"
                     /translation="LFRSGLRLFVYALALGSLYVANTWDYPTYLLIVLVALALPMLGT
                     TTARWAFADGEGDAAGPASGLGGRLRAARDTRLGSWVTQAAALVPLSLLLYLPFHLTF
                     KSLVGGESVELPAQVGSLPILGGLATKLSSLIGINIWPKTWQGFITIFGFFLYAIVAL
                     LGIMLVRGILARRAAGEGPGNTPLVLLGGVVAVSLVGAVALQFPLMALLPPLAAVAIY
                     LIRERLQPGRWSSEELFALGLIAVGAAITFGTEIFFLQDVFHSRFNTLFKFYYQVWIL
                     WGLAAAYATWWLLSRAFRRATEPGHEGPPVAAKALAGAWGLGFAVLFGLAMIYPALAP
                     AARENGVVWLPFVQVANPAAHQMRGLDGIAWMGDSAPGDLAAIRWLRANAPGTAGIAE
                     ATFNYEYNTSGNHGRVSAYTGMPTITAWPGHEYQWRGGQPAIRAQIDPRQADLNELYQ
                     TTDVARAQQIMQQYGIRYVFVGTIEKGEQGRAPGDTGPWRYSPQALSKFGTFMQPVFS
                     QDGTTVYMLPTASAGAAPNQANPGALPHP"
     gene            1838..>2056
                     /locus_tag="VF276_08720"
     CDS             1838..>2056
                     /locus_tag="VF276_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007568620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HEX6606981.1"
                     /translation="MQIDLNCDMGESFGAWPLGQDAALMPLITSANIACGFHAGDPLV
                     MHRTLAAAAARGVNVGAHPSLHDLWGFGR"
BASE COUNT          303 a          673 c          727 g          353 t
ORIGIN      
        1 gctcttccga tctggcctgc gcctgttcgt ctacgcactg gcgctcggct cgctctacgt
       61 cgccaacacc tgggattatc ccacctacct gctgatcgtc ctggtcgccc tcgccctgcc
      121 catgctgggc acgacaacag cgcgctgggc cttcgcggac ggcgaggggg atgcggccgg
      181 cccggcgtcc gggctgggtg ggcggctgcg ggcggcgcgg gatacgcgcc tgggcagttg
      241 ggtgacgcag gcggcggcgc tggtgccgct gagcctgctg ctctacctgc cgttccatct
      301 gaccttcaag tcgctggtcg gcggcgagag cgtcgaattg ccggcgcagg tgggcagcct
      361 gccgattttg ggagggctgg cgacgaagct gtcgtcgctg atcggcatca acatctggcc
      421 caagacgtgg caaggcttca tcaccatctt cggcttcttc ctctatgcca tcgtggcgct
      481 gctaggcatc atgctggtgc gcggcatcct ggcgcggcgc gcggcggggg aggggcccgg
      541 caacacgccg ctggtactac tgggcggcgt ggtcgccgtc tcgctggtcg gggcagtggc
      601 gctgcaattc ccgctcatgg cgctgctgcc gccgctggcc gctgtggcga tctacctgat
      661 ccgcgaacgg ctgcaaccgg gccgctggtc cagcgaggag ttgttcgcgc tgggactgat
      721 cgccgtaggt gcggcgatca ccttcggcac ggagatcttc ttcctgcagg acgtgttcca
      781 tagccgtttc aacacgctgt tcaagttcta ctaccaggtc tggatcctct ggggcctggc
      841 cgcggcctat gcgacgtggt ggctgctgtc gcgggccttc cgccgtgcaa cggaaccggg
      901 gcacgaaggc cctcctgtgg cggcgaaggc gctggccggg gcgtgggggc tgggcttcgc
      961 cgtgctgttc gggctggcga tgatctaccc ggcgctggcg cccgccgcgc gggagaacgg
     1021 ggtggtctgg ctgcccttcg tccaggtggc gaaccccgcc gcgcaccaga tgcgtggcct
     1081 cgacggcatc gcctggatgg gcgacagcgc gccgggcgac ctcgccgcga tccgctggct
     1141 gcgggccaac gcgcccggca cggcgggcat cgccgaggcg accttcaact acgagtacaa
     1201 caccagcggc aaccatggtc gcgtctcggc ctacacgggc atgccgacga tcaccgcctg
     1261 gccgggccac gagtatcagt ggcgcggcgg tcagcccgcc atccgcgccc agatcgatcc
     1321 gcgccaggcc gacctgaacg agctttacca gacgaccgac gtagcccgcg cgcaacagat
     1381 catgcagcag tacggcatcc gctacgtctt cgtcggcacc atcgagaaag gcgagcaggg
     1441 ccgcgcgccg ggcgacaccg gcccctggcg ctacagtccg caagccctga gcaagttcgg
     1501 cacgtttatg cagccggtct tcagccagga cggcaccact gtctacatgc tacccaccgc
     1561 gtccgccggg gccgcgccca accaggccaa ccccggcgcg ctgccgcatc cttgagcgag
     1621 tggtcagtgg tcagtggtca gtggtcagta gttctcgggt gaagcattgt cactctgagc
     1681 ggagcgaaga gtctcgcgga gggcgggtga cgagatgctg agcgcagcga agcatgacaa
     1741 tggaggcttg tcactctgag cggagcgaag agtctcgcgg gggcaggaga cgagatgctt
     1801 cactgcgttc agcatgacag ggggaaggac gggggagatg cagatcgacc tgaactgtga
     1861 catgggcgag agcttcggcg cgtggccgct ggggcaggat gccgcgctga tgccgctcat
     1921 cacctcggcc aacatcgcct gcggcttcca cgcgggcgat ccgctggtca tgcaccggac
     1981 attggctgct gcggctgcgc gcggagtcaa tgtcggcgcc catccaagtc tgcacgattt
     2041 gtgggggttc gggcgc
//
LOCUS       DASUYC010000531         2347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1169000, whole genome shotgun sequence.
ACCESSION   DASUYC010000531 DASUYC010000000
VERSION     DASUYC010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169000"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VF276_08725"
     CDS             <1..409
                     /locus_tag="VF276_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606982.1"
                     /translation="AAGNPWAADNSRVKLEPNGDLDIRIRGLVVAPGLTAAGLPVNPA
                     FIGRNPVAQVAIAVTWAVPQGVSPTAVFIQQTPLLPLDPNGDLEARVNIGPPPPGAER
                     PIILVRAFGGPYIGLSDFVSDLGDRHPQDFDGD"
     gene            complement(630..2183)
                     /locus_tag="VF276_08730"
     CDS             complement(630..2183)
                     /locus_tag="VF276_08730"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEX6606983.1"
                     /translation="MANSVTRPGRPMSTPVRSAHPTALPSAAVLESTLRELVASSLAV
                     SPTEPAVPAVSSRDPGRPAVLPAAVLWTGLLVGILRGFSSQREIWQLLCVHGLWESPH
                     FPITDAAFYQRLARTPATALQTLFVQVTQALQARFAYHTVGAALAGRPLAAFAAGIFA
                     FDHTHLAGVARRLRALRDVPAGDDRLLPGALGACFDLRRQQWTKALFTTQTTGNLART
                     HAPLPASEPAGETVAAAGTGGAPVGGDATAGALLAGLPVGSLILADLGFFSFAWFDQL
                     STAGYWFVSRLRERVTYVTCHVLYHGTAAGVTVRDSLIYLGKHRADRAGQPVRLVEIT
                     LGQQHYTYLTNVLDPRQLPVWEVQELYRRRWNIEQVFDLLKTHLGLHLLWSASLPVVQ
                     QQVFATLIIAQIVLALRTEVATAAGADVREVSLPLLVRWLPRLATMGRDPLAEFIAAG
                     RRAGYIRPFRGKQWVVPEIAAEHYTVPEAPPPPRPPRYGSRDYHRRKCAETGLSRRQR
                     CKAVRQTVI"
BASE COUNT          393 a          782 c          833 g          339 t
ORIGIN      
        1 ggccgccggc aacccgtggg cggcggacaa cagccgcgtg aagctggagc cgaacggcga
       61 cctggatatc cgcatccgtg gcctggtggt ggcgccgggg ttgaccgcgg cgggcctgcc
      121 cgtcaatccg gcgttcatcg gcaggaatcc cgtggcccag gttgcgattg ccgtcacctg
      181 ggcggtgccg cagggcgtgt ccccgacggc ggtgttcatc cagcagacgc cgctgctgcc
      241 cctggacccg aacggcgacc tggaggcgcg ggtcaacatc gggccacccc cgcccggcgc
      301 ggagcggccc atcatcctgg tccgcgcgtt cggcggtccg tacatcggcc tctcggactt
      361 cgtttccgac ctgggcgacc ggcatccgca ggacttcgac ggggactgac ctcctccgct
      421 cctcgcgaag tctgggggag cgcctgcggg cgctcccccg ttcgcgttgg gggatggagt
      481 ggcgggtaga gcgagcaggg ttcagggttc ggggttcaga ttttgccgcc cgggagaggc
      541 gcgcgacggg cagtggcgtc cgcttcacga ggtcgcctgc gagcccacgc cgacggaatg
      601 gcgcggcggc catctgtacc aacaaaatgc tagattacgg tctgacgtac ggctttgcag
      661 cgctgacggc gcgacaggcc ggtttccgcg catttccggc ggtggtagtc acgcgagcca
      721 tagcgggggg gccggggcgg cggcggcgcc tcgggaaccg tgtagtgttc cgccgcgatc
      781 tcgggcacga cccactgttt cccgcggaac gggcggatgt agccggcgcg ccgaccagcg
      841 gcgatgaact ctgccagggg gtcgcgcccc atcgtagcca gccgcggcaa ccaccggacc
      901 aggagcggca aggaaacctc gcgcacgtcg gcgcccgccg cggtggcgac ttcggtgcgc
      961 agcgccagga cgatctgcgc aatgatcagc gtggcaaaca cctgctgttg gaccaccggc
     1021 agggaagcgc tccacagcaa gtgcaggccc aggtgcgtct tcagcaaatc aaagacttgt
     1081 tcgatattcc agcggcgccg atacagttcc tgcacttccc acaccggcaa ctgccgcggg
     1141 tccagcacgt tggtgagata ggtgtagtgc tgctgcccga gcgtgatttc taccaggcgc
     1201 accggttggc ctgcccgatc cgcgcggtgc ttgcccagat agatgaggct gtcgcgcacc
     1261 gtcacccccg ccgccgtgcc gtgatagagc acgtgacacg tcacgtaggt cacccgctcc
     1321 cgcaagcggc tgacaaacca gtagccggcg gtgctcaact ggtcgaacca ggcgaaactg
     1381 aagaagccca ggtccgccag gatcaagcta ccgaccggca gcccggcgag gagggccccc
     1441 gccgtggcat ccccgcctac cggagccccg cctgtccccg cggcggccac cgtctcgcca
     1501 gcgggctcgc tagccggcag gggcgcgtgc gtccgggcga gattccctgt ggtctgcgta
     1561 gtgaagagcg cctttgtcca ctgctggcgc cgcagatcaa agcaagcgcc cagggcaccg
     1621 gggaggaggc gatcatcgcc tgccgggacg tcccgcagcg cccgcaggcg gcgcgctacc
     1681 cccgccagat gcgtgtggtc aaaggcaaag atcccggcag caaaggcggc gaggggtcgc
     1741 ccggcgaggg ccgcaccgac cgtatggtag gcaaagcgtg cctgcagcgc ctgggtcacc
     1801 tggacaaaga gcgtctgcag ggcggtagcg ggcgtgcgcg ccaggcgctg gtagaacgct
     1861 gcgtccgtga tcgggaagtg ggggctctcc cacaggccgt gcacgcagag caactgccag
     1921 atctcccgct gcgaggagaa gccgcggaga atgccgacca ggagcccggt ccagagcacg
     1981 gcggcgggca gcaccgccgg gcggccgggg tcgcgcgagg acacggcggg caccgcgggt
     2041 tcggtgggac tgacggccag agacgaggcg acgagttctc ggagggtgct ttccagcacc
     2101 gcggcggacg ggagtgccgt agggtgcgcg gagcggaccg gggtagacat cggacgacca
     2161 ggacgggtga cactatttgc cacctcccgc tcccataccc accaagccac tctcacactc
     2221 ctaaccttct cgccgtgcat ggcgatctct gtcagatacc attttgttgg tacagatggc
     2281 acggcggcca ttccctactg ggcaaggaaa cgaggcactg cggaagcagc cgggcgctta
     2341 tgaagta
//
LOCUS       DASUYC010000532         5116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1286159, whole genome shotgun sequence.
ACCESSION   DASUYC010000532 DASUYC010000000
VERSION     DASUYC010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5116
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1286159"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VF276_08735"
     CDS             complement(<1..577)
                     /locus_tag="VF276_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606984.1"
                     /translation="MLAPRVAKLVLLLPVLVLLAGCSLGPAATVTPQAPATPAPATSA
                     PATIPPSLSGNIEATATAIATRPNPLPAEATATGVPSATPAGEAAILPTGTLVPPTLV
                     GVGPTPTYAAEPRWPTDAPPTPFPPPQAIPGAADLALPAAPALSAFAAGLRPDAVDDL
                     TGQESCSVYQIAMRLDPANQQVLGRERVTITN"
     gene            complement(740..3652)
                     /locus_tag="VF276_08740"
     CDS             complement(740..3652)
                     /locus_tag="VF276_08740"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEX6606985.1"
                     /translation="MQPRLGLAALIGLVLFVVLLVSGSPQTSAHAQADTPALTASHAP
                     LGALGARAPLTTTTPVMTTPTSTATATPVPCEVGEWNLRAPYPTPIAGAAGVAQDGIL
                     YNFGGALPTTVLTDSYKYTPATNTWAAIAPLPAPRLVAGAVSDGTYIYILGGTIGQGA
                     TNTFWRYDPVQDTYLALASSPVSVNSPAMAYLNGKIYRIGGCTAGNGLCTGDEIDTVD
                     VYTIATGTWAPAAPYPLARSSIAATALSGYIYTAGGVTQSPWFTGSPKTYRYDPATNT
                     WDDTAIADLPGSITDVVSGMLNGRWILAGAGQGGSFAGAWDPGTDTWTLLPSPPLAHD
                     FASGGSTGQAFYVVGGFDPNDTWQADTQEYREPPCPPTPTVTGTPPTATATATASPTV
                     TPASSPTATQTPLPCGVGAWTLRTPYPTAVAGAAGVIKDGILYNFGGTSTDGIAVLTD
                     TYKYTPGADTWTPLAPLPAPRVVASAVSDGTYIYILGGWGTQGPTNTFWRYDPAQNTY
                     AALAPATLNVGGAALVYVNGKIYRIGGCTVRNGDCSDGQTDTVDVYTIATDTWVPAAS
                     YPLTRGHISATVRNGYIYTAGGVDASGIGTPKTYRYDPAGNTWDDAAIADLPGAITDT
                     VNGILNGRWILAGPGFAVAWDPDADTWTLLPFPLVRADHASGGSTGDAFYVVGGFDLS
                     LNWHTWHAETQEYREPACPPTPTITGTPTAPPTVTATATNPPATAPPGTPTPPMTATA
                     TVPAASPTATATPCGITFSDVQPSDYFAAAVHDLACRGVISGYADGTFRPYNLTSRAQ
                     MVKIVVLGFGYPIQTPPPDRYTFADVPPAHPFWSVIETAAATGIVSGYTCGGPGEPCD
                     GDHRPYFRPYSLVTRGQLAKIVVGGAGWPVLNPPGATFADVDPAHPFYGFVETAAAHG
                     VISGYDCGGSGEPCDPAHRPYFRPANDATRGQIAKIVDLALPQAAGPLPSVPGLLR"
     gene            4050..5039
                     /gene="deoC"
                     /locus_tag="VF276_08745"
     CDS             4050..5039
                     /gene="deoC"
                     /locus_tag="VF276_08745"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEX6606986.1"
                     /translation="MTTTLDRAPVTVEVHPRNPGTPLDLGAVQAVRVNRSAVERRAAT
                     MGTRRTVKKNWQAAWLLRAITCIDLTTLAGDDTPGNVRRLCAKARQPVRQDVLEALGV
                     GDLHLTVGAVCVYHNLVPVAVEALAGSGIPVAAVSTGFPAGQTPLHLKIEEIKESVAA
                     GAKEIDIVISRAHVLTGNWSALYDEVAAFREACGPAHMKTILATGELATLRQVGQASL
                     VCMQAGADFIKTSTGKESVNAVLPVGLVMVRAIRDYFEQTGHRVGFKPAGGIRTAKES
                     LEWLILMKEELGDAWLRPALFRLGASSLLGDIERQLSHHVTGHYAAAHHNPLA"
BASE COUNT          850 a         1635 c         1841 g          790 t
ORIGIN      
        1 ggttggtgat cgtcacccgt tcgcggccca gcacctgctg attcgccggg tccagccgca
       61 tggcgatctg atagacgctg cagctttcct gccctgtcag atcgtccacc gcgtcgggcc
      121 gcagcccggc ggcgaaggcg ctcagcgcgg gcgcggcggg cagcgccaga tcggcggcgc
      181 cggggatggc ctgcggcggc gggaacggcg tgggcggcgc atcggtcggc caccgcggct
      241 cggcagcata cgtcggcgtc gggccgacgc ccaccagcgt gggcgggacc agggtgccgg
      301 tgggtaaaat cgcggcctct ccggcaggag tcgcagaggg cacgccggtg gcggtagctt
      361 ccgctggaag cggatttggc cgcgtcgcta tcgctgtcgc cgttgcctcg atattgccac
      421 tgagggaagg cgggatggtg gccggggcgc tggtagccgg cgccggggtc gccggggcct
      481 gtggcgtcac cgtcgccgcg ggacctagcg aacagccggc cagcagcacc agcacaggca
      541 gcagcaacac gagtttggcg acgcgggggg ctaacacaaa gggtctcctg cgggatgaga
      601 tcggaattgc ggacgtgcct tgaatgaaac gagaaccagc ccgttttagc acgcgcgtgc
      661 gggttcgaga aacgcaacgg cgcgcgcccg aatactccgg gcacgcgccg cattatgcgc
      721 tacagcagat tcgccctcgt cagcgcaaca accccggtac gctgggcaag ggaccagcgg
      781 cctgcggaag cgccaggtcg acgatcttgg cgatctgccc gcgggtggcg tcgttggcgg
      841 ggcggaagta gggccggtgg gccggatcac agggctcgcc ggacccgccg cagtcgtagc
      901 cgctgatcac cccgtgcgcc gccgccgttt ccacgaagcc gtagaaaggg tgggcgggat
      961 cgacatcggc aaaggtggcg ccgggcgggt tgagcaccgg ccaacctgcc ccgcccacca
     1021 cgatcttcgc caactggccg cgcgtcacca ggctataggg gcggaagtag gggcggtgat
     1081 cgccgtcgca cggttcgccg ggcccgccgc acgtgtaacc gctcacgatg cccgttgccg
     1141 ccgccgtctc gatcacggac cagaaggggt gcgctggcgg cacatcggcg aaggtgtagc
     1201 ggtcgggcgg aggcgtctgg atcgggtagc cgaagcccag cacgacgatc ttgaccatct
     1261 gcgcgcggct ggtcaggttg tagggccgga aggtgccgtc ggcgtagccg gagatgacgc
     1321 cccggcaggc caggtcatgc accgccgccg cgaagtagtc gctgggctgc acatcgctga
     1381 aggtgatgcc gcagggcgtc gcggtcgccg tcggcgaagc ggcgggcact gtggcggtgg
     1441 cagtcatagg gggcgtgggt gtccccggcg gcgccgtcgc tgggggattg gtggccgtcg
     1501 ccgtcaccgt gggcggtgct gtcggcgtgc cggtgatggt gggcgtgggc gggcaagccg
     1561 gctcacgata ctcctgcgtc tccgcgtgcc aggtgtgcca gttcaggctc agatcgaagc
     1621 cgcccacgac ataaaacgcg tccccggtgc tgccgccgct tgcatgatca gcgcgtacaa
     1681 gtggaaacgg caacagggtc caggtgtccg cgtcgggatc ccaggctacg gcgaaccccg
     1741 gcccggccag aatccagcgc ccattcagta tcccattgac ggtatcggtg atcgcaccgg
     1801 gcagatcggc aatcgccgca tcatcccagg tgttaccggc cggatcgtag cgataggttt
     1861 tcggcgtacc aataccggat gcgtctacgc ccccggcggt atatatgtac ccatttcgca
     1921 cagtggccga gatatgaccc ctggtgagcg ggtaggatgc cgccggcacc caggtatcgg
     1981 tagcgatcgt atagacatcc accgtgtcgg tctgtccgtc ggaacaatcg ccgttgcgca
     2041 ccgtgcagcc cccgatgcgg tagatcttac cgttcacgta gacgagggcc gccccaccca
     2101 cgttcaacgt agccggagcc agtgccgcat acgtgttctg cgccgggtca tagcgccaga
     2161 aggtattcgt aggaccctgt gtcccccatc caccgaggat ataaatgtag gtgccgtcgc
     2221 tcacggcgga ggcgacgacg cgcggcgccg ggagtggtgc gagcggcgtc caggtatcgg
     2281 cgccgggtgt gtatttatag gtatcagtca ggaccgcgat tccatctgtg ctcgtcccgc
     2341 cgaagttata aaggatgccg tcctttataa cccccgcggc tccggcaacc gccgtcggat
     2401 agggcgtgcg cagggtccag gcgcccactc cgcagggcaa gggcgtctgg gttgccgtgg
     2461 gcgaactcgc cggtgtcacg gtaggcgaag cggtcgccgt cgcggtggca gtgggcggcg
     2521 tgccggtgac ggtcggggtg gggggacacg gcggttcgcg gtactcctgc gtatccgcct
     2581 gccaggtatc gttgggatca aagccgccca ccacataaaa cgcctgcccc gtgctgccgc
     2641 cgctcgcgaa atcatgggcc agcggcggcg agggcagcag cgtccaggtg tctgtacccg
     2701 gatcccaggc tccagcgaaa gaaccgccct gccccgcccc tgccaggatc caacgtccgt
     2761 tcagcatccc gctgaccaca tcggtgatgg agccggggag gtcggcaatg gcggtgtcat
     2821 cccaggtgtt ggtcgccgga tcgtagcgat acgtcttggg ggagcctgtg aaccagggag
     2881 actgggtaac gcccccggcg gtatatatgt acccactcaa cgcagtcgcc gcgatggaac
     2941 ttcgggcgag gggatagggt gccgccggcg cccaggtgcc ggtggcgatc gtgtagacat
     3001 ccacggtgtc gatctcgtcg ccggtacaaa gcccgttgcc tgccgtacag ccgccgatcc
     3061 ggtagatctt gccgttcaag taggccatgg ccgggctatt cacactaacc ggcgaggaag
     3121 ccagggccag gtaggtgtcc tgcaccgggt catagcgcca gaaggtgttg gtcgcaccct
     3181 gcccgatggt accgcccaga atatagatat aggtgccgtc gctgacggca cccgcgacca
     3241 gacgaggcgc cggcaggggt gcaatcgccg cccaggtgtt ggtggctggg gtgtatttat
     3301 aggagtccgt tagcaccgtg gtggggagcg cgccgccgaa gttatagaga atgccatcct
     3361 gcgcgactcc ggcagccccg gcaatgggcg tgggataagg cgcgcggaga ttccattcgc
     3421 ccacctcgca aggcacgggc gtcgcagtcg ccgtgctggt cggggtcgtc atgacggggg
     3481 tggtggtcgt gagcggggcc cgcgcgccca gcgcgccgag cggggcatgg gaggccgtca
     3541 gggccggggt atcggcctgg gcatgggcgg aggtttgcgg cgatccgctc accaggagca
     3601 cgacgaacag gacgaggccg atcagggcgg cgagaccaag acgcggttgc atatactctc
     3661 cctggaaagc gacaggggtg cccatctgca cccggtgacg tgccggagga agcaacggag
     3721 agcgcgcccg gtccgatccc gcgggcccgg cacgataccc gcacaggatg ttcggagaaa
     3781 cactgctgat tatagcagaa aactcgcgcc ctgtacagac aggtgctaga cggagggcga
     3841 atttcgtttc ccgcgtggaa gaatcccgtt ccactatata agcgggcgct tgtgccggga
     3901 gccgcgccac ccggcgctgc agccccgcgc ggctgccggg cttgacaagc aggccgcgtt
     3961 tgcgggacaa tgggatctac ctgagcgttg tggctcggca taacttcgca tcagcaacgt
     4021 ggccgcgggc ggccaggagg ataacggcaa tgacaaccac gctggatcgc gcgccggtga
     4081 cggtggaggt tcacccgcgc aaccccggca ctccgctgga cctgggcgcc gtgcaggcgg
     4141 tgcgtgtgaa ccgcagcgcc gtcgagcgcc gggcggcgac gatgggcacc cgccgcaccg
     4201 tgaagaagaa ctggcaggcg gcctggctgc tgcgcgccat cacttgcatc gacctgacca
     4261 cgctggccgg cgacgacacg ccgggcaacg tgcgccggct ctgcgccaag gcccgccaac
     4321 ctgtgcgcca ggatgtgctg gaggccctcg gcgtcggcga cctgcatctc accgtgggcg
     4381 cggtctgcgt ctatcacaac ctggtgccgg tggccgtgga ggcgctggcc gggtccggca
     4441 tcccggtggc cgccgtgtcc accggcttcc ccgccgggca gaccccgctg cacttgaaga
     4501 tcgaggagat caaggagtcg gtcgcggcgg gcgcgaaaga gatcgatatt gtgatctccc
     4561 gcgcccatgt gctgaccggc aactggagcg cgctctacga cgaggtggcc gccttccgcg
     4621 aagcctgcgg cccggcgcac atgaagacga tcctcgccac cggcgaactg gcgacgctgc
     4681 gccaggtggg ccaggccagc ctggtctgca tgcaggcggg cgccgacttc atcaagacct
     4741 ccaccggcaa ggagtcggtc aacgccgtgc tgcctgtggg cctggtcatg gtgcgggcca
     4801 tccgcgacta tttcgagcag acgggccacc gggtgggctt caagcccgcc ggaggcatcc
     4861 gcacggccaa ggagtcgctc gaatggctga tcctgatgaa ggaagagctg ggcgacgcgt
     4921 ggctgcgccc ggccctgttc cgcctgggcg ccagcagcct gctgggcgac atcgagcgcc
     4981 aactgtcgca ccacgtcacc ggccactacg cggcggcgca ccacaacccg ctggcctagc
     5041 ggcgagtgcg ctgccggggc gggccggttt gaaaccggcc cgcccagcgg gatggagtgc
     5101 atgaatatgg ccgcca
//
LOCUS       DASUYC010000533         2428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1050587, whole genome shotgun sequence.
ACCESSION   DASUYC010000533 DASUYC010000000
VERSION     DASUYC010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050587"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VF276_08750"
     CDS             complement(<1..481)
                     /locus_tag="VF276_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6606987.1"
                     /translation="MPTILRFALYLVAFATILVATLGSGMLTDSRLAGAQQQLAAAYQ
                     QQSTLLRGQVAAIQAHSAVAGYLNGDRAGRDLALSQRTELSRAAAEYESIAPGAAATH
                     YTEVVTRFNAALDHVFAVYDTGGLRLARPALTTGLDPVARDLAAGHDQMLARAGADVG
                     "
     gene            996..>2428
                     /locus_tag="VF276_08755"
     CDS             996..>2428
                     /locus_tag="VF276_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX6606988.1"
                     /translation="MSGTAKLDALRQALGSGAILARNTVLQSRYEIEQVLGIGGMSTV
                     YRARDLRFTNVLRYCAVKEMPDTAPDPKTGALRFSTFEREASLLATLSHAGIPKIYDF
                     FSQDSRVYLVLEYVAGKDLEVLLDEAGGPLPETQVLRWAVQVCDVLAYLHAHRPQPIV
                     FRDLKPSNIMVNEAGDKITLVDFGIAKAFQSDKKGTMIGTEGYSPPEQYRGLAEPRGD
                     IYALGATLHHLLTNSDPRTETPFTFHERPVRQLNPAVSPDTEALIMRALEYDASRRWE
                     SAEAMRAAILAILEPQPAAPAVGTDPGARPTASGAPPAPPAAPAYAVVPPGQPAAYPY
                     TMPPGQVPGQMLPGQPVPGMAYPGMMPPPYGAAPGVPAPVVDATTKQVWVFTAEDEIR
                     SSPAVAKGMVFVGCYDSNLYAVNAKTGEFVWKRATEGGIPSSPVVWEDLVIVGSEDTS
                     IYALDTRRGAVQWTAGTGAPVRSSPRVS"
BASE COUNT          371 a          869 c          819 g          369 t
ORIGIN      
        1 cgcccacgtc ggcgccggcg cgggccagca tctggtcgtg gccggcggcc aggtcgcggg
       61 cgacagggtc caggccggtg gtcagggcgg ggcgcgccaa gcgcagaccg ccggtgtcgt
      121 agacggcgaa gacatggtcg agggcggcgt tgaagcgggt caccacctcg gtataatgcg
      181 tggcggcggc gcccggcgcg atgctctcgt actcggcggc ggcccggctt agctcggtgc
      241 gctggctcag cgcgaggtcc cgcccggccc ggtcgccgtt gagatagccg gcgacggcgc
      301 tgtgcgcctg gatggccgcc acctggccgc gcaagagggt gctctgttgc tggtaggcgg
      361 cggcaagctg ctgctgggcg cccgccagcc ggctgtcggt gagcatgccg ctgcccaacg
      421 tcgccaccag gatcgtggcg aaggcaacca ggtataaggc aaagcgcaga atcgtcggca
      481 taaggcgcag gacagatccc ggcgcgcggg ggcgggcggg gatcggactc cagtgctcta
      541 ttatacaaca gcgggcgggg cggcgccgcc acccggcggt cacgcacgcc cacacgctat
      601 tgcaatgtac ttttagttcg cgtataattg ggaaaagccg ccgccgtccg aggtttggtc
      661 aatgtgctgc gcgccgcgat ggtgaggcga accgggtttc gcaacgggtg ggcacctttt
      721 ttcatctgga ctcatctttt ctcgcgcacc tgtccgcccc gactgtccct gttccctcca
      781 ccggtgtggc gccgtggcgg atctgcccct cgcagcggat acggcgcgcc gcgcattgcg
      841 ctcgtgctac gcatcgtacc agtctggagg ccgccgtgct catcaagaag ctgcctgggg
      901 gcgacgggcc gaatcgtccc tatgacccga acggcagtcg tccgctcgat ccgggcggga
      961 gccgggcctt gccggcatcc gatcccgccg atcagatgag cggtacggcc aaactcgacg
     1021 ctctgcgcca ggccctcggc agcggggcca tcctcgcccg caacaccgtg ctccagagcc
     1081 gctatgagat cgagcaggtg ctgggcatcg gcggcatgag caccgtctac cgggcgcgcg
     1141 acctgcgctt caccaacgtg ctgcgctatt gcgccgtcaa ggagatgccc gatacggcgc
     1201 ccgaccccaa gaccggcgcc ctgcgcttct cgacgttcga gcgcgaagcc tcactgctgg
     1261 cgacgctcag ccacgccggc atccccaaga tctacgactt cttctcgcag gacagccgcg
     1321 tttacctggt gctggagtat gtggccggga aggacctgga ggtgctgctc gacgaagcgg
     1381 gcggccccct gccggagacc caggtgctgc gctgggcggt gcaggtctgt gatgtgctgg
     1441 cctatttgca cgcgcaccgg ccccagccca tcgtgttccg cgacctcaag ccgtcgaata
     1501 tcatggtgaa cgaggccggc gacaaaatca cgctggtcga cttcggcatc gccaaagcct
     1561 tccagtcgga caagaagggc acgatgatcg gcaccgaggg ctactcgccg ccggagcaat
     1621 accgcgggct agccgagccc cgcggcgaca tttacgcgct cggcgcaacc ctccatcacc
     1681 tgctgaccaa cagcgacccg cgcacggaaa cgccgttcac ctttcacgag cgccccgtgc
     1741 gccagttgaa ccccgccgtc tcacccgaca ccgaagcgct gatcatgcgc gcgctggagt
     1801 acgacgcctc gcgccgctgg gagtcggcgg aggcgatgcg cgcggcgatc ctggcgatcc
     1861 tggagccgca accggcggcg cccgccgtgg gcaccgaccc cggtgcccgc cccaccgcct
     1921 ccggcgcccc gcccgcgccg cctgccgcgc ccgcctacgc cgtggtgccg cccggccagc
     1981 cggcagccta tccctacacc atgccgcccg gccaggtgcc ggggcagatg ctgccgggcc
     2041 aacccgtgcc gggcatggcc tatcccggca tgatgccccc gccgtatggc gccgcgcccg
     2101 gcgtcccggc ccccgtggtg gacgcgacca ccaagcaggt ctgggtcttt accgccgaag
     2161 acgagatccg ctcctcgccc gcggtagcca aaggcatggt ctttgtcggc tgctacgaca
     2221 gtaacctcta cgccgtcaac gccaaaaccg gcgagtttgt ctggaagcgg gccacggaag
     2281 gcgggattcc atcctcgccg gtggtctggg aagacctggt tatcgtcggc tccgaggaca
     2341 ccagcatcta cgcgctcgac acccgccgcg gcgccgtgca gtggacggcg ggcaccgggg
     2401 cgcccgtccg ctcgtcgccg cgggtctc
//
LOCUS       DASUYC010000534         2653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_391728,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000534 DASUYC010000000
VERSION     DASUYC010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391728"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="VF276_08760"
     CDS             complement(<1..743)
                     /locus_tag="VF276_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[LysW]-aminoadipate kinase"
                     /protein_id="HEX6606989.1"
                     /translation="MYVVKMGGAAGVGTDALCADLAALHRAGQQVVLIHGGSDETNRL
                     AEALGHPPRFVTSVSGHTSRVTDPRTLEIFVMATAAINRRLVAGLQARGVPAVGLSGI
                     DGRLIEARRKEAIRVVEDGRQRIIRDDWTGTPTRVNVALLQTLLDAGYVPVLAPLAIS
                     PAGETLNIDGDRGAAALAGALGAEALVLLTNVPGLLRAYPDEGTLIRHIPRAALDEVA
                     GAAAGRMRKKLLGAAEALDAGVPRVIIADG"
     gene            complement(897..1934)
                     /gene="argC"
                     /locus_tag="VF276_08765"
     CDS             complement(897..1934)
                     /gene="argC"
                     /locus_tag="VF276_08765"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX6606990.1"
                     /translation="MRVRVSIAGGSGYVGGELLRLLLSHPHVEIGQVTAERRAGEFVY
                     HAHPNLRGHTDLRFVPADALEPCDLLLLALPHGHAARAIDRLSALAPRLVDCSADFRL
                     GAAATYERWYGTPHPAPAWLGRFTYGLPELHREAIREAPYVSGVGCNATAVNLALLPL
                     VRAGVLDATRGVIAEVKAGSSEGGNEASAASHHPERSGAVRSFAPVGHRHTAEVQAAL
                     GLDAVHLSVTSIDLVRGALATCHAFLREPLRDRDLWRIYRQTYGGDPFVRIVHDRDGI
                     YRHPEPKILAGSNHADVGWALDETTGRIVALCAIDNLMKGAAGTAVQCLNLMCGFAEN
                     AGLGFPGLHPV"
     gene            complement(1934..>2653)
                     /gene="lysX"
                     /locus_tag="VF276_08770"
     CDS             complement(1934..>2653)
                     /gene="lysX"
                     /locus_tag="VF276_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysX"
                     /protein_id="HEX6606991.1"
                     /translation="RAFPYDTVFERSISYGRSLYAIRTLESWGVRCLNRAEVIATCGD
                     KLLTSLALERDGIPTPRTAVAFTPEAALAAIEEMGYPAVIKPVVGSWGRMVARVNDRD
                     AAEAVLEDRATLGSWQQQVVYIQEYVAKPGRDIRAFVVGDEPICAIYRTSEHWITNTA
                     RGGRASNCPVCGPVGETALRAARAVGGGILAIDLVEAPDGRLQVIEVNHTMEFRNSID
                     TTGVNIPARMIDYLLAQGEAA"
BASE COUNT          371 a          944 c          949 g          389 t
ORIGIN      
        1 ccatcggcga ttatcacgcg gggcacgccc gcgtccagcg cttcggcggc gcccagcagt
       61 tttttgcgca tccggcccgc cgccgccccg gccacctcgt ccagcgccgc ccgcgggatg
      121 tggcggatca gcgtgccttc gtcggggtag gcgcgcagca ggcctggcac gttggtcagc
      181 aggaccagcg cttcggcgcc cagcgccccg gccagcgcgg cggcgccccg gtcgccgtcg
      241 atgttcagcg tttcccccgc cgggctgatc gccagcgggg ccagcaccgg cacgtacccg
      301 gcgtcgagca acgtctgtag cagggccacg ttcacgcggg tgggtgtgcc ggtccagtcg
      361 tcgcggatga tgcgctgccg cccgtcctcg accacgcgga tcgcctcctt acggcgggcc
      421 tcgatcagcc gcccgtcgat gcccgacagc ccgaccgcgg gcacgccgcg cgcctgcaac
      481 ccggccacca gccgccggtt gatcgccgcc gtcgccatga cgaagatctc cagcgtgcgc
      541 gggtcggtga cccggctggt gtggccggag accgaggtga cgaagcgcgg tgggtggccc
      601 agcgcctccg ccagccggtt ggtctcgtcc gagccgccgt ggatcagcac gacctgttgc
      661 cccgcccggt gcaaggcggc caggtcggcg caaagcgcgt ccgtgccgac accggcggcg
      721 ccgcccattt tcacgacata catcgctgct ttcctccctc tcacgaacgg agattcttcg
      781 ctgcgctcag aatgaccgag agaacttgtc actctgagcg cagcgaagag tctcgtcggt
      841 tgggagacgg agattcttcg ctgcgctcag aatgacaagg tctggttggc ggactactag
      901 accgggtgca ggccggggaa acccagcccg gcgttctcgg cgaagccgca catcaggttc
      961 aggcactgca ccgcggtgcc cgccgcgccc ttcatcaggt tgtcgatggc gcagagggcc
     1021 acgatgcggc ccgtcgtctc gtccagcgcc cagcccacgt cggcgtggtt gctgcccgcc
     1081 aggatcttgg gctcggggtg gcgatagatg ccgtcgcggt cgtggacgat gcgcacgaag
     1141 gggtcgccgc cgtaggtctg gcggtagatg cgccagaggt cgcggtcgcg cagcggctcg
     1201 cgcaggaagg cgtggcaggt tgccagcgcc ccgcgcacga gatcgatgga tgtgaccgac
     1261 aggtggaccg cgtccaggcc gagcgccgcc tgcacctcgg cggtgtggcg gtggccgacc
     1321 ggggcgaacg agcgcaccgc gccgctgcgt tccgggtggt ggctggccgc gctggcctca
     1381 ttgccgccct ccgacgaccc ggccttgacc tcggcgatca cgccgcgcgt cgcatccagc
     1441 acgcccgcgc gcaccagcgg cagcagcgcc aggttgacgg cggtggcgtt gcagcccacg
     1501 ccgctgacgt agggcgcctc gcggatggcc tcacggtgca gttcgggcag gccgtaggtg
     1561 aagcggccca gccaggcggg cgccgggtgc ggcgtgccgt accagcgctc gtaggtggcg
     1621 gcggcgccca ggcggaagtc ggcggagcaa tcgacaaggc gcggcgccag ggcgctcagg
     1681 cggtcgatgg cgcgggcggc gtggccgtgc ggcagggcta ggagcagcag atcgcacggt
     1741 tccagtgcat ccgcgggcac aaagcgcagg tcggtgtgcc cgcgcaggtt ggggtgggcg
     1801 tggtagacga actcgcccgc gcgccgctcg gctgtcacct ggccgatctc gacgtgcggg
     1861 tggctcaaca gcagccgcaa cagttcgccg ccgacgtagc cggacccccc ggcgatggat
     1921 acccggactc tcatcacgcc gcctccccct gcgccagcag atagtcgatc atgcgcgccg
     1981 ggatgttgac cccggtggta tcgatactat tccgaaactc catcgtgtgg ttgacctcga
     2041 tcacctgcaa gcgcccgtcg ggcgcctcga ccagatcgat cgccaggatg ccgccgccga
     2101 ccgcgcgggc cgcccgcagc gccgtctcgc cgaccggccc gcagacgggg cagttcgagg
     2161 cccgcccgcc gcgcgccgtg ttggtgatcc agtgctcgct ggtgcggtag atggcgcaga
     2221 tcggttcgtc gccgacgacg aaggcgcgga tgtcgcggcc cggcttggcg acatattcct
     2281 ggatataaac cacctgctgc tgccacgagc cgagggtcgc gcggtcttcc agcaccgcct
     2341 ccgccgcgtc gcggtcgttc acgcgggcga ccatgcggcc ccacgagccg accaccggct
     2401 taatcaccgc cgggtagccc atctcctcga tggcggccag cgccgcctcc ggggtgaagg
     2461 ccaccgcggt gcgcggcgtc gggatgccgt cgcgctccag ggccaggctg gtcagcagct
     2521 tgtcgccgca ggtggcgatc acctcggcgc ggttcaggca gcgcacgccc cagctttcta
     2581 gcgtgcggat cgcgtagagc gagcgcccgt agctgatcga gcgctcaaag accgtgtcat
     2641 acgggaaggc gcg
//
LOCUS       DASUYC010000535         3018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1205286, whole genome shotgun sequence.
ACCESSION   DASUYC010000535 DASUYC010000000
VERSION     DASUYC010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205286"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="VF276_08775"
     CDS             complement(<1..843)
                     /locus_tag="VF276_08775"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6606992.1"
                     /translation="MKKLLGNRLTIMVGVALLLVAGSLLFAPTIAGPLTPAPVTDPAG
                     VIAAGVTPPQGYADRIIGDMKTRLQSNANDPTSLAQLGLAYLQKARETSDPTFYTQAE
                     TVLRKALAINPGSFDATAAIGSLELSRHQFREALDWGRKAQALAPQKAYAYGVIGDAQ
                     IELGDYDQAVATFQQMIDLRPDLSSYARVSYARELYGDVEGAIQAMRQAAAAGGPAPE
                     NAGWAHVQLGNLNFNSGRLDKAEHEYNAALVVYPGYFHAQAGLAAVRAAQGRSAEAVA
                     LYKAA"
     gene            complement(990..2588)
                     /locus_tag="VF276_08780"
     CDS             complement(990..2588)
                     /locus_tag="VF276_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015102812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HEX6606993.1"
                     /translation="MKLAKIGSTLLALLVVFGMFAAGMRSGPVSASSHREAPMISNDP
                     VADDTDFYAFVSPDKPDTVTFIINTWPFEEPVGGPNYFTFGDDVLYELHIDNVGDAQS
                     HITYQFRFKTTVGNGNTFLYNTGPIKSLDDPNWNVKQTYTVTRLDNGKATVLGENLKT
                     PPVNVGAKSTPNYEALSNAAIQSLSDGSKVFAGQADDSFFVDLNVFDLLTIRKLPGNA
                     GGGVDGLKGYNVQTIALQVPMTRLTADGTRPADAKAANAVIGSWTTTSRQATTVLRGN
                     GTAEHSGDWVQVSRLGAPLVNEVVAPLAAKDLFNSSKPADDAQFLGAVTDPEMGRLFK
                     ALYNIQVPPTPRDDLVAIFLTGIPGATMPPNVKPSEQLRLNMGVPPAANPNPLGVVGG
                     DLAGYPNGRRLADDVTDISLKAVAGAAYPLFHPDFKADALAAQLGDGVDANDVPFRSS
                     FPYVALAWDGTTSVPHGAYAPSTGGAQPPMAGSPGMPRTGATDSGHLADIYAWLLALV
                     AVAGGLILAGGWTVRRRATAQAKK"
BASE COUNT          462 a          982 c         1047 g          527 t
ORIGIN      
        1 cgccgctttg tagagcgcca ccgcctccgc gctgcgcccc tgggccgcac gcacggcggc
       61 cagcccggcc tgggcatgga agtagccggg gtagaccacc agcgccgcgt tatactcgtg
      121 ctcggctttg tccagccgcc cgctgttgaa attgagattg ccaagctgga catgggccca
      181 ccccgcattc tccggcgcgg gaccgcccgc cgccgccgcc tggcgcatgg cctggatggc
      241 gccctcgaca tcgccgtaga gttcgcgcgc gtaggacacc cgcgcatacg aactgaggtc
      301 gggccgcaga tcgatcatct gctgaaaggt cgcgaccgcc tggtcatagt cgcccagttc
      361 gatttgcgcg tcgccgatga ctccgtaggc ataggctttt tgcggggcga gagcctgggc
      421 tttgcgtccc caatcgagcg cctcgcggaa ctggtggcgc gacagctcca gcgagccgat
      481 ggccgccgtc gcgtcgaagc tgccgggatt gatcgccagg gccttgcgca ggaccgtttc
      541 ggcctgcgta tagaaggtcg ggtcgctggt ttcgcgggct ttctgcaagt acgccaggcc
      601 caactgggcc agagaggtag ggtcgttggc gttcgactgc aagcgcgtct tcatatcgcc
      661 gatgatgcgg tcggcgtacc cctgcggcgg cgtcaccccg gcggcgatca cacccgccgg
      721 gtcggtcacc ggcgcgggcg tgagaggccc ggcgatcgtg ggcgcgaaca gcaggctacc
      781 cgcgacaagc agcagcgcca cgcccaccat aatcgtcagt cgattgccca gtagtttctt
      841 cacgaggttg cgcgcctcct tggttccagt tggatccgga tgcggagcat gttttgcgtt
      901 gcgctacacc ggtcacttat cgcatctgtg cgtaattttt gccgtgcgag ccgggcgcag
      961 gtcacagcgc ctgcgcccgg cttgcctgct tacttcttgg cctgggccgt ggcgcgacgg
     1021 cgcaccgtcc agccaccggc caggatcagg cccccggcta cggcgacgag cgccagcaac
     1081 caggcgtaga tgtcggccag gtggccgctg tcggtcgcgc cggtgcgcgg cataccgggc
     1141 gagccggcca tcgggggctg ggcgccgccc gtgctgggcg cgtaggcgcc gtgcggcacg
     1201 gaggtcgtgc cgtcccaggc cagggccaca taggggaagc tgctgcggaa cggcacgtcg
     1261 ttggcatcca cgccgtcacc caactgcgcg gcgagcgcat cggccttgaa gtccgggtgg
     1321 aacagcggat aggcggctcc cgcgacggcc ttgagcgaga tgtcggtcac gtcgtcggcc
     1381 aggcggcgcc cgttcgggta gccggccagg tcgcctccga ccacacccag aggattcggg
     1441 ttggccgcgg gcggcacgcc catgttcagg cgcagttgtt ccgacggctt cacgttcggc
     1501 ggcatggtcg cgccggggat gccggtcagg aagatcgcca ccaggtcgtc gcgcggggtg
     1561 ggcggcacct ggatgttata gagcgccttg aacagccggc ccatctccgg gtcggtcaca
     1621 gcgcccagga actgggcatc atcggcgggc ttggagctgt tgaacaggtc cttggcggcc
     1681 agcggggcca ccacttcgtt gaccaggggc gcgccaaggc ggctcacctg cacccagtcg
     1741 ccggagtgct cggccgtgcc gttgccgcgc agcaccgtcg tggcctggcg gctcgtcgtg
     1801 gtccacgacc cgatgaccgc gttggcggcc ttcgcgtcgg cgggcctggt gccgtcggcg
     1861 gtcaggcggg tcatcggcac ctggagcgcg atggtctgca cgttgtagcc cttcagcccg
     1921 tccaccccgc cgccggcgtt gcccggcagc ttgcggatgg tcagcaggtc aaacacgttg
     1981 aggtcgacga agaaggaatc gtcggcctgc ccggcgaaga ctttgctgcc gtcgctcaag
     2041 ctctggatcg cggcgttgga gagcgcctca tagttcgggg tcgacttcgc gccgacgttc
     2101 acgggcgggg tcttgaggtt ctcgcccagc acggtcgcct tgccgttgtc gaggcgggtc
     2161 acggtgtagg tctgcttcac gttccagttc ggatcgtcca ggctcttgat cgggccggtg
     2221 ttgtagagga aggtgttgcc gttgccgacc gtggtcttga agcggaactg ataggtgatg
     2281 tgggactggg catcgcccac attgtcgatg tgcagttcat acagcacatc atcgccgaag
     2341 gtgaaataat tggggccgcc gaccggttcc tcgaacggcc acgtgttgat gatgaaggtg
     2401 acggtgtccg gcttgtccgg gctgacaaaa gcgtagaagt cggtgtcatc cgccaccggg
     2461 tcattgctga tcattggggc ttcgcggtgg ctggaggcgc tcaccgggcc ggaacgcata
     2521 ccggctgcaa acataccgaa gaccaccagc aatgcgagca gggtactacc aattttcgcg
     2581 agtttcatca ggaaatctcc tcttcctctc cgacccccgg agcggagaaa cagtccccag
     2641 gttctgtgat ccccccgaag taccgtaccg ggtccgaggt cgtccaaacc gtgcgcgcag
     2701 ggcgccggca cggtcgggcc gtggactcgc tgcccctgga ccgggtgtgg tatccgccgc
     2761 gcgccggtcg cgcgcatcgc acctcctttc acacgatggc tctccggaga agccagggca
     2821 gacaaagtgt ctgcgagtta tacccgcgaa cagggcaacc ggattgggcc gggggtgcct
     2881 gcggcgcggg ccaggcaggt acccccgctg cggctagcgc cggccccgca tcagggccag
     2941 ccccagcccg atataccagg ccggattggc gagaaacccg gccagcagcg cgggcacgac
     3001 aatgatcagg ttggccgg
//
LOCUS       DASUYC010000536         3601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1548348, whole genome shotgun sequence.
ACCESSION   DASUYC010000536 DASUYC010000000
VERSION     DASUYC010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1548348"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="VF276_08785"
     CDS             complement(<1..403)
                     /locus_tag="VF276_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6606994.1"
                     /translation="MHFPITDGIILQREVGKVRAVDGLDFTVKRGETLGLVGESGCGK
                     STTGRAILQLYRPSAGHVYFEGQDLTKMSGEQLRQQRRQMQMIFQDPYASLNPRMKVT
                     EIVGEAPLVHGLVRRAGLDAYVDAQLRRAGLD"
     gene            complement(531..1526)
                     /locus_tag="VF276_08790"
     CDS             complement(531..1526)
                     /locus_tag="VF276_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6606995.1"
                     /translation="MAALLQVKDLKTQFFTQDGVVNAVNGVTFDLYEEETLGIVGESG
                     SGKSVTALSLMGLIPQPPGKITNGEVLFKGKNLVGLPEDEMRKIRGKEIAMIFQDPMT
                     SLNPVLTISRQIGEALQLHMGMDRAAARKRTIELLKLVGIPSAADRIDDYPHQFSGGM
                     RQRVMIAMALSCNPKLLIADEPTTALDVTIQAQILDLIKRLKREFGTAVMMITHDLGV
                     VAGMCDRVQVMYAGHLVEQASVEQLYADPRHPYTLGLIRSVPRLDEARKERLEPIPGL
                     PPDLVNVPPGCPFYPRCSFHMPVCLEQRPLLRPVDGAHTAACWADIKEAVMTNAS"
     gene            complement(1684..2622)
                     /locus_tag="VF276_08795"
     CDS             complement(1684..2622)
                     /locus_tag="VF276_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6606996.1"
                     /translation="MAVINASDVDTAIDFARPGKARSLWSDAWARLRRNKLALGGAIV
                     LLLMAFIAVAAPVIAPYPYDKTVRDPVTKKVVPNLPPAWAEGGKSQYLFGTDEQSRDE
                     FSRVVYGAQVSLSVGLVSQIIILCIGIPIGLIAGYYGKGVDMVLMRITDIMYAFPTVL
                     FAVVILAALGPSLFNIYAAIGFTFWPPMARLVRSQVLSLKEKEYVEAAQAIGVKAGRI
                     MFAQILPNALGPIIVAVTFGIPFAILIEAFLSFIGIGVPIPIPSWGAMIARGRDQLRS
                     DPWLIIFPSAALSLTLFAFNFFGDGLRDALDPRSRR"
     gene            complement(2627..>3601)
                     /locus_tag="VF276_08800"
     CDS             complement(2627..>3601)
                     /locus_tag="VF276_08800"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6606997.1"
                     /translation="WPNFAGAGAEVWRIAVLVLGIVAIAAGLFLRRRRVSLQLPGAAL
                     ITTGGLFLFWSVLMFIEGPVTKAQASGLVPGQFLRFAWNVLHLDLGTSFSQVGRPVTD
                     IIVSAAGNSFLLGLCAFLILICLGLPLGILAAVRQNSWIDYMVSAISLVGYSIPNFVL
                     GALLILVTVLWLPFLPTAHWGDPIDLLFPALVLAIRPMAVLMRLTRASMLEVLNQDYI
                     RTAWSKGLAGRAVLIRHALRNALLPVVTVMGDQLGDLVTGSLVVETIFNVPGIGQYFV
                     KSVDSLDYGMIMGTTLFYAALVMIINLTVDLMYGVIDPRVRLGRGART"
BASE COUNT          727 a         1135 c         1152 g          587 t
ORIGIN      
        1 gatcaaggcc ggcgcggcgc aattgcgcgt ccacataggc atcgagcccg gcgcggcgca
       61 ccaggccatg caccagcggc gcctccccca cgatctccgt caccttcatg cgcggattga
      121 gcgaggcata ggggtcctgg aagatcatct gcatctggcg gcgctgctgg cggagttgtt
      181 cgccgctcat cttggtcagg tcctggccct cgaagtagac gtgcccggcg ctggggcggt
      241 agagctgcag gatagcgcgg cccgtggtcg acttcccgca gccggactcg cccaccagac
      301 cgagtgtttc gccgcgcttc acggtgaagt ccagcccgtc cacggcgcgc actttgccga
      361 cctcgcgctg caggatgatg ccgtccgtga tcgggaagtg catcttcagg ccctcgacgc
      421 gcaggatcgt atcggccccc ggccccgtgc cgttggtcga cggcccacta gtcgcggtgc
      481 tttgcgcgct gcgcatcgtg ccggtggcgg cgcggtccgt cgtcggttcg ttagctggca
      541 ttcgtcatca ccgcctcttt aatgtcggcc cagcaggccg cggtatgggc gccgtccacc
      601 gggcgcaaca ggggccgctg ctccaggcac acgggcatat ggaagctaca gcgcgggtag
      661 aagggacaac ccggcggcac gttgaccagg tcgggcggca gaccggggat cggctccagc
      721 cgctctttgc gcgcctcgtc gagccgcggc accgagcgga tcaggcccag cgtgtagggg
      781 tggcgcgggt cggcgtagag ttgttccacg ctggcctgct cgaccaggtg cccggcgtac
      841 atcacttgca cccggtcgca catcccggcc acgacgccga ggtcgtgggt gatcatcatc
      901 accgccgtgc cgaactcgcg cttcaggcgc ttgatcaggt ccaggatctg ggcctggatg
      961 gtcacgtcga gggcggtggt cggctcgtcg gcgatgagca gcttggggtt gcaactcaag
     1021 gccatggcga tcatcacgcg ctggcgcata ccgccggaga actggtgcgg gtagtcgtcg
     1081 atgcgatccg ccgccgaggg aatgccgacc agcttcaaca actcgatcgt ccgcttgcgc
     1141 gccgccgccc ggtccatgcc catatggagc tgcagcgctt cgccgatctg gcggctgatg
     1201 gtcagcaccg ggttgagcga ggtcatcggg tcctggaaga tcatggcgat ctccttgccc
     1261 cgaatcttgc gcatctcgtc ttcgggcaag cccaccaggt tcttgccttt aaacaacacc
     1321 tcgccattgg tgatcttgcc gggcggttga gggataaggc ccataagcga aagagcggtt
     1381 acgctcttgc cggaaccgct ctcacccaca atacccaggg tctcttcctc atatagatcg
     1441 aaagtgacac cgttgacggc attgacgacg ccgtcctggg tgaagaactg agttttaaga
     1501 tctttaacct gtagtagcgc cgccatacgg cctccatcta gtacgaactc gccgacgaac
     1561 cgggacccag ggccggagag taaatcctgg ggccacggcc caagccggcc ccggtcgcgc
     1621 ctatttatct agaacgcagg tcaggggcgc gtgatgcgcc gggctgccct ggaaagcaca
     1681 aggctatctc cggctccgcg ggtccagcgc atcgcgcaac ccgtcgccga aaaagttaaa
     1741 ggcgaaaagc gtgagcgaca acgccgcgct cgggaagata atcagccacg gatcggatcg
     1801 taactggtcg cgcccgcggg cgatcatggc gccccaactg ggaatgggga taggcacccc
     1861 gataccgatg aagctcaaga aggcttcgat caggatggcg aaggggatgc cgaaggtcac
     1921 ggccacgatg atcggcccca gggcattagg caaaatctgg gcgaacatga tgcggcccgc
     1981 cttcacaccg atggcctgag cggcttccac atactccttc tctttgagtg acagcacctg
     2041 gctacgcacc aggcgggcca tgggcggcca gaaggtgaag ccgatagccg cgtagatatt
     2101 gaataagctg ggaccgagcg cggccaggat gaccaccgcg aacagcacgg tggggaaggc
     2161 gtacatgatg tcggtgatac gcatcaggac catatcgacg cccttgccat agtacccggc
     2221 gatcagcccg atcgggatgc cgatacacaa gatgatgatc tggctgacca ggcccacgga
     2281 gagcgaaacc tgtgcgccgt agaccacccg gctgaactca tcgcgggact gctcatcggt
     2341 gccgaacagg tattgcgatt tgccgccctc ggcccaggcc ggcggcaagt tgggcaccac
     2401 cttcttggtc acggggtcgc gcacggtctt gtcatagggg tagggagcaa tcaccggcgc
     2461 ggccacggcg atgaaggcca tcaggagcag cacgatggcc ccgccgagcg ccagtttatt
     2521 gcggcgcagg cgcgcccagg catcgctcca caagctgcgg gccttgcccg gtcgcgcgaa
     2581 atcaatcgcg gtatcgacat cggaagcatt gattacggcc atagtcttag gtcctcgctc
     2641 cccggcctag ccgcacccgc gggtcaatca cgccgtacat cagatccacg gtcaggttga
     2701 tgatcatgac cagcgcggca tagaagagcg tggttcccat tatcatacca taatccaggg
     2761 aatcgacgct cttgacgaaa tactggccga tgcccggcac gttaaagatc gtctcgacga
     2821 ccaggctccc ggtgacgagg tcgccaagct ggtcgcccat gacggtcacg accggcagca
     2881 gcgcgttacg cagcgcatgg cggatcagca cggcccgccc ggcgagacct ttcgaccagg
     2941 cggtgcgaat gtagtcctgg ttcaagacct ccagcatact ggcgcgggtg aggcgcatca
     3001 agacggccat gggccggatg gcgagcacca gcgcgggaaa caagagatcg atggggtcgc
     3061 cccaatgtgc ggtgggcagg aacggcaacc agagcaccgt caccagaatg agcaaggcgc
     3121 cgagcacgaa attgggaatg gaatagccga ccaacgagat cgcgctgacc atgtaatcga
     3181 tccagctatt ctgccgcacg gcggcgagaa taccgagcgg caagccgaga cagatcagga
     3241 tcaggaaagc gcagaggccc agcaggaagg aattccccgc ggcgctgacg atgatgtcgg
     3301 tgacgggccg gcccacctgg ctgaacgagg tgcccagatc gagatgcagc acgttccagg
     3361 caaaacgcag aaattggccg ggcacaagtc cactggcctg cgccttcgtg accggacctt
     3421 caatgaacat cagcaccgac cagaacagga acagcccgcc cgtggtgata agggccgcgc
     3481 cgggcaattg caaactgacc ctgcggcggc gcaagaagag accggcggca atcgccacga
     3541 tccccaatac gaggacggct atccgccaga cttcagcgcc tgcgcccgca aaattaggcc
     3601 a
//
LOCUS       DASUYC010000537         2315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1423102, whole genome shotgun sequence.
ACCESSION   DASUYC010000537 DASUYC010000000
VERSION     DASUYC010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423102"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /locus_tag="VF276_08805"
     CDS             <1..1146
                     /locus_tag="VF276_08805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6606998.1"
                     /translation="PLLFACGGAMLIRRDVFLAVGGFDPSFFIYFEDVDLGWRLWVLG
                     HRVVFAPGSIVYHKEGGTTGAKRAPSHRRYLLFEANTLCAVFKNYGDEAENRVLPAAL
                     LLELQRALLAAGDAVDPEHYRLGGSAGAGAPDNGHPAALPPMTVAHLLALRRLDERLP
                     HLVAERARIQAARRRPDSAILPLFRRPFQPQFAGARYADAMRRIAAALDLYPLVAGAA
                     PSRVLLLSPDSGPDRARAAALAALLGAEFLAQTATSTATNGGWTLPPIPGPRPDGFPP
                     PTAPEVAATADLLLAVGAAVPAAAPLAPAVPLAADVATRRPDSISPAWRATLAQRAGL
                     LICADPATATAWHAALPDLPVAVQPDGAPLAATLRHYCRYPLHGRPA"
     gene            1160..1720
                     /locus_tag="VF276_08810"
     CDS             1160..1720
                     /locus_tag="VF276_08810"
                     /inference="COORDINATES: protein motif:HMM:NF021081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2378 family protein"
                     /protein_id="HEX6606999.1"
                     /translation="MQQPASRPTDQVVYHAALESLTRAVQADQQPALIEELRTRCGYD
                     LRRPQREYPPDVARKVLDLLTERCFPDRPRVEAYEEFGRRAFAAYRATVVGRVIMAAL
                     HMATTFHALQLAAQAFKSVSNYTRFEAIPDGPRAAVFRAHHSILHPRYMLGLLDGLLA
                     ASGHPDAQITVDSTHAPDLVDYHVSW"
     gene            1788..>2315
                     /locus_tag="VF276_08815"
     CDS             1788..>2315
                     /locus_tag="VF276_08815"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX6607000.1"
                     /translation="MAYVITPMDEEAARDIAGWRYAPPFAFYNSAPGALPALLDPANA
                     YYAVRTADGMLAGFFCFGAEAQVPGGHRQGLYTGQDTLDIGLGLRPDLTGHGQGLAFV
                     QAGLDFARRTFAPARFRLSVATFNTRAIRVYERVGFQPGPVFHSPTSEGDTEFLLMSR
                     EAGSDASQDHERHDHS"
BASE COUNT          290 a          948 c          746 g          331 t
ORIGIN      
        1 ccgctgctgt tcgcctgcgg cggcgccatg ctgatccgcc gcgacgtgtt cctggccgtg
       61 ggcggcttcg accccagctt tttcatctac ttcgaggatg tcgatctcgg ctggcggctg
      121 tgggtgctgg gccaccgcgt ggtcttcgcg cccggttcca tcgtctacca caaggagggc
      181 ggcaccaccg gcgccaaacg cgccccctcg caccggcgct acctgctgtt cgaggccaac
      241 acgctctgcg ccgtcttcaa gaactacggc gacgaagccg agaatcgcgt gctgccggcg
      301 gccctgctgc tggaattgca gcgcgccctg ctggccgcgg gcgacgccgt cgatcccgaa
      361 cactaccgcc tgggcggcag cgcgggcgcc ggcgcgcccg acaacggcca ccccgccgcc
      421 ctgccgccga tgaccgtggc ccacctgctg gcgctgcggc gcctggacga gcgcctgccg
      481 cacctggtgg ccgagcgcgc ccgcatccag gccgcccgcc gccgccccga cagcgcgatc
      541 ctgcccctgt tccggcggcc cttccagccg cagttcgccg gggcgcgcta cgccgatgcc
      601 atgcgccgca tcgccgccgc cctcgacctc tacccgctgg tggccggggc cgcgcccagc
      661 cgcgtgctgc tgctgagccc cgattccggc cccgaccggg cgcgcgccgc cgcgctggcg
      721 gccctgctgg gcgccgagtt cctggcgcag acggcgacca gcaccgcaac caacggcggt
      781 tggaccctgc ccccgatccc cggcccgcgc cccgacggct tcccgccgcc caccgcgccg
      841 gaggtcgccg ccaccgccga cctgctcctg gccgtgggcg ctgccgtgcc cgccgccgcc
      901 ccgctggccc ccgcggtgcc gctggccgcc gatgtggcga cccggcgccc cgacagcatc
      961 tcccccgcct ggcgcgcgac gctggcccaa cgcgccgggc tgctcatctg cgccgatccg
     1021 gccacggcga cggcctggca cgccgccctg cccgatctgc cggtcgccgt ccagccggac
     1081 ggtgccccgc tcgctgcgac tctccgccat tattgccggt atccgctgca tgggaggccg
     1141 gcatgaaagg gagcgcccga tgcaacaacc cgcgagtcgc cccaccgatc aggtcgtcta
     1201 tcacgccgcg ctggaaagcc tgacccgcgc cgtccaggcc gaccagcagc ccgcgctcat
     1261 cgaggaactg cgcacgcgct gcggctatga tctgcgccgg ccccagcgcg aatacccgcc
     1321 cgatgtggcc cgcaaggtcc tggatctgct caccgagcgc tgcttccccg atcggccccg
     1381 cgtcgaggcc tacgaggagt ttggccgccg cgccttcgcc gcctaccgcg ccaccgtcgt
     1441 gggccgcgtg atcatggccg cgctgcacat ggcgacgacg ttccatgcgc tgcaactggc
     1501 cgcccaggcg ttcaaatcgg tctccaacta cacccgcttc gaggcgatcc ccgacgggcc
     1561 gcgcgccgcc gtcttccgcg cgcaccacag catcctgcac ccgcgctata tgctgggcct
     1621 gctcgacggc ctgctggcgg ccagcggcca ccccgacgcc cagatcaccg tggactcaac
     1681 ccacgcgccg gatctggtgg attatcatgt aagctggtag gcgcggcgcc cgcggaccgc
     1741 gctatcatgt aattgggccg ccgggcaggc gcaaaggagg tgtgggcatg gcatacgtca
     1801 tcactccgat ggacgaggag gccgcgcggg atatcgccgg ctggcggtat gcgccgccgt
     1861 ttgcgttcta caactcggcg cccggcgcgc tgcccgcgtt gctcgacccg gccaacgcct
     1921 actacgccgt gcggaccgcg gacggcatgc tggccggctt cttctgcttc ggcgccgagg
     1981 ctcaggtgcc gggcggccac cgccagggtc tctataccgg ccaggacacg ctcgacatcg
     2041 gcctgggcct gcgccccgac ctcaccggcc acggccaggg cctggccttt gtgcaggcgg
     2101 ggctcgactt cgcccggcgc acgttcgccc cggcgcgctt ccgcctgagc gtcgccacct
     2161 tcaacacccg cgccatccgc gtctacgagc gggtcgggtt ccagcccggc cccgtcttcc
     2221 acagccccac cagtgaaggc gataccgagt tcctgctgat gtcccgcgag gccggttccg
     2281 acgcatcgca ggaccacgag cgccacgacc atagc
//
LOCUS       DASUYC010000538         3574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_132300,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000538 DASUYC010000000
VERSION     DASUYC010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132300"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VF276_08820"
     CDS             complement(<1..765)
                     /locus_tag="VF276_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607001.1"
                     /translation="MGGRTTHWPIAAPAGQDSGHLSSYQARLFSMLLRWTFLVCLLLA
                     ASNLGQFVATRVWPAALLSVAYGAETVFVGLLWRHVRRTADTHLLTLYIITTMGVIAL
                     TMMFVVDAIIIGNALGLCIMLLLAIVWQPRERMVYWTGLAVMLYVTAVGVRGSGWFPV
                     IPPGPLAWSGLYFSPALGLILSAGVMQNFADRLRQALAQSEHARDALAESHQALSART
                     RELEAERSALRTALEEIRERQRAELALVQAKEVAEAA"
     gene            complement(1127..2092)
                     /locus_tag="VF276_08825"
     CDS             complement(1127..2092)
                     /locus_tag="VF276_08825"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX6607002.1"
                     /translation="MAGEFNHQSADNEFERPQARGANEAARAVDASADAAPAVLAHVA
                     AHGAGSARGNGGARATAVQRMQQMYGNRAVQRVLQRAAGTEADEDIGSRIQARAGQGS
                     NLDAGVQRRLESGLGADLSSVRVHTDHEADHLARSVEAVAFTTGSDIYFRAGTYNPGT
                     PEGLHLLAHEATHTVQQAAGPVAGTPAAGGVAISDPGDSFEQAAEQAAARVAHGAPAA
                     PAAPAAGEAAGAAVQREAAPEEEEPLQAMRPATYAAVQREAAPEEEEEPLQTMRPATY
                     AAVQREAAPEEEEPLQTMRPLTYAAVQRTESADEAGEQEQEEAAN"
     gene            2644..3150
                     /locus_tag="VF276_08830"
     CDS             2644..3150
                     /locus_tag="VF276_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607003.1"
                     /translation="MELEYRLLTPGERWPEDAASLAAAIRSQGPFQPVVEMAGPDLRV
                     HDAVTGLTSHARLTPYPAQTYESLIQPPGYAPRMTDRDRDTALPFQRTVEIRTSAAPD
                     SDPTTVALFHATVTDGLWWLLGGFLADPYRRTVWGWRRWRGDQPLAAHAAETAAHLAA
                     PVPEGGPA"
     gene            3147..3410
                     /locus_tag="VF276_08835"
     CDS             3147..3410
                     /locus_tag="VF276_08835"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEX6607004.1"
                     /translation="MSHASGAPEVVLYTLPYCPDCRDLRRLLNERQVSFTEVDLARTP
                     GAVESMLKINGGRRSAPTVRIGTQVLVDPAAGEIDAALQTYAR"
BASE COUNT          535 a         1325 c         1178 g          536 t
ORIGIN      
        1 ggcggcttcg gccacctctt tcgcctggac cagcgccagt tcggcccgct gccgctcgcg
       61 gatctcctcc agggccgtgc gcaaggcgct gcgctcggcc tccaattcgc gggtgcgcgc
      121 gctcagcgcc tggtgcgact cggccagggc gtcgcgggcg tgctcgctct gcgccagcgc
      181 ctggcgcagc cggtcggcga aattctgcat gacgccggca gacaggatga ggccgagcgc
      241 cgggctgaaa tacagcccgc tccaggccag cgggccgggc gggatcaccg ggaaccaccc
      301 gctaccgcgc acgcccaccg ccgtgacgta gagcatcacc gccaggcccg tccagtagac
      361 catgcgttcg cgtggttgcc agacgatcgc cagcagcagc atgatgcaca accccagggc
      421 attgccaatg atgatggcat cgaccacgaa catcattgtc agcgcgatca cgcccatcgt
      481 ggtaatgatg tagagggtga gcagatgggt gtcggcggtg cgccggacat ggcgccacag
      541 gagcccgacg aacaccgttt cggcgccgta ggccacgctc agcagcgccg cgggccacac
      601 tcgggtcgcg acaaattgcc ccaggttcga cgcggcgagg aggaggcaga ccaggaaggt
      661 ccagcgcagt agcatcgaaa acaaccgggc ctggtagctg gatagatgcc cgctatcctg
      721 cccggccggc gcagcaatgg gccagtgggt ggtccgtccc cccataatat gatctccgtt
      781 ccagccctgc cggggcaggg atcgatccag ggctccccga tgcacgccgg gcgcctcccg
      841 ttgattatag gcaaaatcgg cccccggctg ccagagccga caagcccaat ccaggatcac
      901 agcgcatcct gccatcgggc cggcagagat acctaccgca cagcgcaagg gggtcgggta
      961 acgatgggca gacggcggaa aattgcgggg gtctccggag ggcgacggaa aacccgtggc
     1021 ccccggcgct tcgggggcca cgcggcaaag ccagttggca ggtatgaggt cgggccggga
     1081 agggcaaccc cgcccgcgcc gtgcccgacc aggcgggcgc gtccgcctag ttcgctgctt
     1141 cttcttgttc ctgttcgccc gcctcatcag cggactctgt ccgctgcaca gcagcatagg
     1201 tcagcgggcg catggtctga agcggttctt cttcctccgg tgcggcctcg cgctgcaccg
     1261 cggcatacgt ggctgggcgc atggtctgga gcggttcttc ttcttcttcg ggtgcggcct
     1321 cgcgctgcac cgcggcatag gtggccgggc gcatggcttg cagcggctct tcttcctcgg
     1381 gtgcggcttc gcgctgcact gccgcgcctg cggcctcccc ggctgcgggg gcagccggtg
     1441 ccgccggcgc accgtgggcc acgcgggcgg cggcttgctc ggcggcctgc tcgaagctgt
     1501 cgcccggatc gctgatcgcc acgccgccag ctgccggggt gccggccacc ggccccgccg
     1561 cctgctgtac ggtgtgggtc gcctcgtggg ccagcaagtg caggccctcc ggcgtgcccg
     1621 gattataggt gcccgcgcgg aaatagatgt ccgacccggt ggtgaaggcc acggcctcga
     1681 ccgagcgggc cagatggtcg gcttcgtgat cggtgtggac gcgcacgctc gacaaatcgg
     1741 cgcccagccc ggactccagc cggcgctgca ccccggcgtc gaggttgctg ccctggccgg
     1801 cgcgcgcctg gatgcggctg ccgatatcct cgtccgcttc ggtgcccgcc gcgcgctgca
     1861 acacccgctg cacagcccga ttgccgtaca tctgctgcat gcgctggacg gcggtggccc
     1921 gcgccccgcc attaccccgc gcgctgccgg cgccgtgcgc ggccacgtgc gccagcaccg
     1981 ccggggccgc atccgcgctc gcatctaccg cccgcgccgc ctcattggcg ccccgtgcct
     2041 ggggccgctc aaactcgttg tcggcggatt ggtgattgaa ctcaccggcc atcacgaaac
     2101 cctccttatg acacctgccc tatggggaaa cgaatggaaa tattatagca ccgaatcgcc
     2161 ggtctgcata ccgagatttg tccaaattga cagattcttt ggggcaggcc cgccgcccgg
     2221 cgcgagtgtc gcccgcgatg cacaccgggg cggcctcgcg ccccggacta ttgtcggggc
     2281 ggccatagcc ataaagtacc atccccgccc gccggatgca ccttcccccg cgcgccggtt
     2341 ttccgctata ctggagtcgc tgccggtgcg ggcatagccc tggcccgacc ccctggagaa
     2401 cccgcggcac cagcagcctg agacctcacc cgcaccagcg cggtgtgtaa ctgacgcatc
     2461 ttccaatgct ctgatcgatc aaggagacct tccccgcccg cgcatggggc ttgcccgtgg
     2521 ccgcctgccg cccatgcgag ccatgccggc accgaccggc ctatccccgc cccgatcccc
     2581 atccccgccc gcgcccggcg aacacggcgc cggagcgtgc cccgtctcgt tttggaggaa
     2641 cgtatggaat tagaataccg cttgctcacg ccgggcgagc gctggcccga agatgcggcg
     2701 tcgctggcgg cggccatccg cagccagggg cccttccagc cggtggtcga gatggccggc
     2761 ccggacctgc gagtccacga cgccgtgacc ggcctgacca gccatgcccg gctcacaccc
     2821 taccccgcgc agacctacga gtccctgatc cagccgccgg ggtacgcccc gcgcatgacc
     2881 gaccgcgacc gcgataccgc gctgcccttc cagcggacgg tggagatccg caccagcgcc
     2941 gccccggaca gcgatcccac caccgtggcg ctcttccacg ccaccgtgac cgacgggctg
     3001 tggtggctgc tgggcggctt cctggccgat ccgtaccggc gcaccgtctg gggctggcgc
     3061 cgctggcgcg gcgatcagcc gctggccgcc cacgccgccg aaaccgccgc gcacctggcc
     3121 gccccggtgc cggaaggagg cccggcatga gccacgccag cggcgccccc gaggtggtgc
     3181 tctacaccct gccctattgc cccgattgtc gcgacctgcg ccgcctgctc aacgagcgcc
     3241 aggtcagctt caccgaggtc gacctggccc gcacgcccgg cgcggtcgag tcgatgctga
     3301 agatcaacgg cgggcggcgc tcggcgccga cggtgcgcat cggcacgcag gtgctggtcg
     3361 atccggcggc cggcgagatc gacgcggccc tgcaaaccta cgcccgctaa ccccgccgcc
     3421 cctctcccgc atcagcctgt gagtagcagc cggtcggcga ccggctgcta ctgcatctcc
     3481 gccgattcgc cgtggctccc ggcccaatag tggccggggc gcgcgacttg ctgtataaca
     3541 gacagtacaa ggagccgcga atcgcaagct atgg
//
LOCUS       DASUYC010000539         3494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_90849,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000539 DASUYC010000000
VERSION     DASUYC010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90849"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2106
                     /locus_tag="VF276_08840"
     CDS             <1..2106
                     /locus_tag="VF276_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607005.1"
                     /translation="DQGRLLLLLGRTGPAVNAFRAAVQRGANDPQLHAGLAQALLVAG
                     DAKGAQAESEKAIAATNLDPAQADLTLTYSKLDNSDPYQRSVGQAVLTANLVRARALA
                     QMGQAGQAHTFATAVEGQGTGGDAAKAGWFRYYAALIDSAGGFADDAVARLRPATPGA
                     VEAGGPSLGARILAWVDMLTPAQARDQAPAIASAMGGQDGVPVLPADAAMAQADFELA
                     HKLDAVGAPAAPFYRVAAAWEAAQAASRHQPPAAGNGTPRPVQYQVAEADYLRRQGTR
                     DPLTLARYQQASDVAPNLAGIHTNRGLAFADLGDQLRMRREFELAGRADSQDAVAAHN
                     LGVLALRDGPANLLTALANLGRARQSAGPAATRWGNSFVADPQPGGYPVPVAAGDSFA
                     DRIPALATLILLLLHTLIPRRRSQGEESRDAPPGSVLARLGDLLAARTRGAIERSIPA
                     LTLTAAALIGGLGWAWALSGNGPSAALTLLPVTILAALVALGAQEGMQWAAVHMERRP
                     GTIRTRLWPLGLILSLVGAPFGVLYGRLLNTHAAGATGTHTVLEAHRDGHEEPEELEP
                     VAAAPAAVAQRAPARRGGTATARPPAPAKTAPVAAPAPERVVWRGWGALGPAARVALL
                     GLLANIVLAVVCGLLAAWLPNAFWRILLLANVAVLAFTAASDPGADGWHLWRRSPLLW
                     LPIFSLAAAALTALLLGAI"
     gene            2380..2592
                     /locus_tag="VF276_08845"
     CDS             2380..2592
                     /locus_tag="VF276_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX6607006.1"
                     /translation="MFEGLLQPTHLILILVIVLIIFGPGKLPELGKTLGTGIREFREA
                     ASRAEEPDPPPPTVETQALPPEQPRS"
     gene            complement(2741..>3494)
                     /locus_tag="VF276_08850"
     CDS             complement(2741..>3494)
                     /locus_tag="VF276_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX6607007.1"
                     /translation="AGLARDTRTIRIGQLVTCNNYRNPALLAKIASTVDVMSHGRLDF
                     GIGAGWYEHEWLAYGYEYPDAPTRLKMLAEALPIIKAMWTEDYATFEGQYYQVRGAIN
                     EPKGVQQPHPPIWIGGGGEKVTLKLVAKYGDACNIGGFDPDVYRHKFDVIRQHCETVG
                     RDYDSILRSSEVFVYLTDHPERLDSEIAHLLPAVQEDIRNRYLVAPPAQVVEKLQSLV
                     DAGVQYFIVYLHNAAHLEPVQRFAAEVVPAFR"
BASE COUNT          501 a         1371 c         1142 g          480 t
ORIGIN      
        1 gaccagggcc ggctgctgct gctgctgggc cgcaccggcc ccgcggtcaa cgccttccgc
       61 gccgccgtgc aacgcggcgc caacgacccg caactccatg ccgggctggc tcaggccctg
      121 ctcgtcgcgg gtgacgccaa aggcgcgcag gcggagagcg agaaggccat cgccgccacc
      181 aacctcgacc cggcccaggc cgatctcacg cttacctata gcaagctcga caactccgac
      241 ccctaccagc gcagcgtcgg ccaggccgtg ctgaccgcga acctggtgcg ggcgcgcgcc
      301 ctggcccaga tgggtcaagc cgggcaggcc cacaccttcg ccaccgccgt cgagggccag
      361 ggcacgggcg gcgacgcggc gaaagcgggc tggttccgct actacgcggc gctcatcgat
      421 tcggcgggcg gcttcgccga tgatgccgtc gcccgcctgc gccccgcgac ccccggcgcc
      481 gtcgaagccg gtgggccaag cctgggcgcc cgcatcctcg cctgggtcga tatgctgacc
      541 ccggcgcagg cccgcgatca ggccccggcc attgcgtcgg ccatgggcgg ccaggacggc
      601 gtgcccgtgc tccccgccga cgcggccatg gcccaggccg attttgaact ggcgcacaag
      661 ctggacgccg tgggcgcccc cgccgcgccg ttctaccgcg tcgccgccgc ctgggaagcc
      721 gcccaggccg ccagccggca ccagccgccc gccgccggca acggcacgcc ccgccccgtg
      781 cagtaccagg tcgccgaagc cgactacctg cgccgccagg gcacgcgcga cccgctcacc
      841 ctggcccgct accagcaggc gagcgacgta gcgcccaatc tggcgggcat ccacaccaac
      901 cgcgggctgg ccttcgccga tctcggcgat cagctgcgga tgcgccgcga gtttgaactg
      961 gcgggccgtg ccgacagcca ggacgccgtc gccgcccaca acctgggcgt cctcgccctg
     1021 cgcgacggcc ccgccaacct gctgaccgcc ctggcgaacc tgggcagggc ccgccagtcc
     1081 gcgggccctg ccgctacccg ctggggcaat agcttcgtgg ccgacccgca gcccggcggc
     1141 tacccggtgc ccgtcgccgc cggagacagc ttcgccgacc gcatcccggc cctggcgacg
     1201 ctgatcctgc tgctgctgca cacgctgatc ccgcgccgcc gcagccaggg cgaggagagc
     1261 cgcgacgcgc cgcccggcag tgtgctggcc cgcctgggcg atctgctcgc cgcgcggacc
     1321 cgcggtgcaa ttgagcgcag tatccccgcc cttacgctga ctgcggcagc tttaataggc
     1381 gggctaggtt gggcctgggc actgtcgggc aacgggccga gcgcggcgct caccctactg
     1441 ccggtcacga tcctggcggc gctggtcgcc ctcggcgcgc aagaaggcat gcaatgggcc
     1501 gctgtgcaca tggagcgccg tccgggcacc atccggaccc ggctctggcc gctcggcctg
     1561 atcctgagcc tggtgggcgc gccgtttggc gtgctctatg gccgcctgct gaacacgcac
     1621 gccgcggggg ccaccgggac ccacaccgtg ctcgaagccc accgcgacgg ccacgaagaa
     1681 cccgaagaac tggaacccgt ggccgcggcc cccgccgccg tggcccagcg cgcgccggct
     1741 cggcgcggcg gcacggccac cgctcgcccg cccgccccgg ccaaaaccgc gccggtcgcc
     1801 gctcccgccc cggagcgcgt cgtctggcgc ggctggggcg cgcttggccc cgcggcccgc
     1861 gtggccctgc tcggcttgct ggcgaacatc gtgctggccg tcgtctgcgg actgctggcc
     1921 gcctggctgc cgaacgcgtt ctggcgcatc ctgctgctgg cgaacgtcgc cgtgctcgcc
     1981 ttcaccgccg cctccgaccc cggcgccgac ggctggcacc tctggcgccg cagcccgctg
     2041 ctctggctgc ccatcttcag cctcgccgcc gccgcgctca ccgccctgct gctcggcgcg
     2101 atctaatcgc gggccgcgcc gggccggttt gaaagcggcc cctacaccgg ccctgtgccc
     2161 aaaccggccc ctacaccggc cccatgttca aacccgactc ccctctccgc ggcggagagg
     2221 ggccggggga gaggttgcgc gggcgatcca aaccgcccgc caaacgagta tactgaatac
     2281 gacgccgccc ccggccacgg aggcggcccg aacaccgtcg cacacatccc ggcccagatc
     2341 cggcccggcc accggcagcg ggcgccgagg aggatcccca tgtttgaagg tctcctgcaa
     2401 cccacccacc tgatcctgat tctggtcatc gtgctcatca tcttcggccc cggcaaactg
     2461 ccggagctag gcaaaaccct gggcaccggc atccgcgagt tccgcgaagc ggcctcccgc
     2521 gccgaggagc cggacccacc cccgcccacg gtagaaacgc aagcgctccc gccggagcag
     2581 ccgcgcagct aaccgcccag gagcaggcat ggcaaacggg ttcccagtat cgggaacccg
     2641 tttctgtttg cggctaactc accaatacac aacgacaccg cgactcccct ctccgcggcg
     2701 gagaggggcc gggggagagg ttggtcgggc gccaccccgc ctaccggaac gcgggcacca
     2761 cctcggcggc gaagcgctgc accggctcca ggtgcgctgc gttgtgcagg tagacgatga
     2821 agtactgcac ccccgcatcc accaggctct gcaacttttc gactacctga gcaggcggag
     2881 ccacgagata ccggttgcgg atgtcctcct ggaccgcggg caagagatgc gcgatctcgc
     2941 tgtccagccg ctccggatgg tccgtcaggt agacgaacac ctcgctggag cgcaggatgc
     3001 tgtcgtagtc gcggcccacc gtctcgcagt gttgccggat cacgtcgaac ttgtgccggt
     3061 agacatccgg gtcgaagccg ccgatgttgc aggcgtcgcc gtatttcgcc accagcttga
     3121 gcgtgacctt ctcgccaccg ccgccgatcc agatcggcgg gtggggctgc tgcacgccct
     3181 tcggctcgtt gatcgccccc cgcacctgat aatattgccc ctcgaaggtg gcgtaatcct
     3241 cggtccacat cgccttgatg atcggtagcg cctcggccag catcttgagc cgggtggggg
     3301 catcggggta ctcgtagccg taggccagcc actcatgctc gtaccagccc gcgccgatgc
     3361 cgaagtccag ccgcccgtgg ctcatcacgt ccacggtgga ggcgatcttc gccagcagcg
     3421 ccgggttgcg gtagttgttg cacgtcacta attggccgat gcggatcgtg cgggtgtccc
     3481 gcgccaggcc cgcc
//
LOCUS       DASUYC010000540         3195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_745470,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000540 DASUYC010000000
VERSION     DASUYC010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745470"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VF276_08855"
     CDS             complement(<1..314)
                     /locus_tag="VF276_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607008.1"
                     /translation="MPEKEDSGIRIRANRADTTGGPAPAPARRPAQPRRQSYQYYLED
                     RPVLVPVVMIAVCSVCVFLTIVTHALRDWLLGPTPSRADLGNYNMAMSWMAAFALAGI
                     FLS"
     gene            524..1885
                     /locus_tag="VF276_08860"
     CDS             524..1885
                     /locus_tag="VF276_08860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEX6607009.1"
                     /translation="MQPSANPVNRPDLPPAPQAPHAPAPLLRPGFGRRLGLALCLALL
                     GAYGLIAVLTVQKAGGPEPYVRQTDFLAALTGGTILREEPPGALYDEPTQTAVQARLL
                     REVGREPLLLLPFNHPPPEALFVALLGGAGLSAPAIFAAWTLCSIAALGLALAALHRA
                     WPVPGAPGALLTLAALSFFPVTGALLLGQSTALVLLGWAAGSAALRRNADAWAGGALA
                     LAVLKPQTVPVLLLALLLMRRGRALLTWIATVAGAAVMLMPFLGADWPLRYLGFVFHT
                     ASGPATAAIDPATMQNWRGLFARLLADTPPAAVLTGIAMVLSLVLVCWIWLRATRPPA
                     AGPAAATAWDRRWAITLLVALLVNPHLQPHDLTLALVPGWILVAHLAPARWWLGWLWA
                     GWVAGFVAMLYNTFLLGPAVLWLIATVALLVRHTLQQPTPTTASGLPQALSALPADSR
                     PPR"
     gene            2042..3094
                     /locus_tag="VF276_08865"
     CDS             2042..3094
                     /locus_tag="VF276_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX6607010.1"
                     /translation="MQVKDYYKTLGVAREATDKEIRSAYRKLARKYHPDVNPNNKEAE
                     AKFKDINEAYEVLSDAEKRKKYDKFGADWEQFEKMGGAPTDFDFSRYGAPGGGRVYTG
                     AAPGGEGFSDFFESLFGGMGARGGVGRDPFGGMGGIGGFGRAVPRRGEDFEHPIEVTL
                     EEAFNGTSRRLQMQTEDNCPTCGGTGMVNNRPCPTCGGSGVVARMKTIEVNIPAGVHT
                     GSRVRVRGEGGPGSEGQPRGDLFLIVTVVPHPRFERKGDDLSEAVAVPLYTMVLGGEA
                     RVPTLKGTNLALNIPAGTANGRTFRLKGQGMPHLGNPQQRGDLLVKAEVELPKQLSGE
                     ERQLFEQLQRIHKEAG"
     gene            3094..>3195
                     /locus_tag="VF276_08870"
     CDS             3094..>3195
                     /locus_tag="VF276_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011049905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX6607011.1"
                     /translation="MSEFGHNDEPRYVISVAAQLLQVHPQTLRLYERE"
BASE COUNT          535 a         1142 c         1044 g          474 t
ORIGIN      
        1 gagaggaaga tcccggcgag agcaaaggcc gccatccaac tcatcgccat gttgtaattg
       61 cccaggtcgg cgcgcgaggg cgtgggtccc agcagccaat cgcgcagcgc gtgcgtcact
      121 atcgtcaaga acacgcaaac gctacagacg gcgatcatga ccacgggcac cagcaccgga
      181 cgatcttcga ggtagtactg gtaactctgg cggcggggtt gggcggggcg gcgagccggc
      241 gccggcgcgg ggccgccagt cgtatcggcg cgattggccc ggatacggat tccgctgtcc
      301 tctttctccg gcacgagatt ctccagacta gcgacgcggt cggatgacag gtgggagcaa
      361 acgccgccta cgatgtagac ggcataggcc aactatacca cccacggtcc cccggcacaa
      421 gcgccgaaag gccgggattc catggcccag gtggccttgc gggcggtggg atccggcgac
      481 tataatgagc acagacacgc cgtcaccttc gcgggaacac gaaatgcagc catccgccaa
      541 cccggtaaat cgccccgatc tgcccccggc gccgcaggca ccccacgccc cggccccctt
      601 gctacgcccc ggcttcgggc ggcggctcgg cctggcactg tgcctcgccc tgctgggcgc
      661 ctacggcctc atcgcggtgc tgacggtaca aaaggcgggg gggcccgagc cgtatgtgcg
      721 ccagaccgat tttctggccg cgctcaccgg cggaaccatt ctgcgcgagg agccgcccgg
      781 cgcgctctac gacgaaccca cgcaaaccgc cgtgcaggcg cgcctattgc gcgaagtggg
      841 ccgcgaaccg ctgctgttgc tgcccttcaa ccacccccca cccgaagccc tctttgtcgc
      901 cctgctcggc ggcgccggcc tcagcgcgcc ggccatcttc gccgcctgga ccctgtgcag
      961 tatcgcggcg ctgggcctcg cgctggccgc gctgcaccgc gcctggcccg ttcccggcgc
     1021 gcccggtgcg ctgctcacgc tggccgcgct cagcttcttc ccggtgacgg gcgcgctgct
     1081 gctcggccag agcacggcgc tggtcctgct cggctgggcc gccggcagcg cggcgttgcg
     1141 gcgtaacgcg gacgcgtggg cgggcggcgc cctggccctg gccgtgctca agccgcagac
     1201 ggtacccgtc ttgctgctcg cgctgctgct catgcgccgg gggcgcgccc tgctgacgtg
     1261 gatcgccacg gtggccgggg cggccgtgat gctgatgccg ttcctggggg ccgattggcc
     1321 gctgcgctac ctgggcttcg tgttccacac ggcgtccggc ccggccaccg ccgccatcga
     1381 cccggccacc atgcagaact ggcgcggcct gttcgcccgc ctgctggccg ataccccgcc
     1441 cgccgccgtc ctcacgggca tcgccatggt cctctcgctc gtgctcgtct gctggatctg
     1501 gctgcgcgcg acgcgccccc cggctgccgg gccggcagcc gccacagcgt gggaccggcg
     1561 atgggcgatc accctgctgg tggccctgct ggtcaatcct cacctccagc cacacgacct
     1621 gaccctggcc ctggtgccgg gctggatcct ggtagcccac ctggcgcccg cccgctggtg
     1681 gctgggttgg ctctgggcgg gctgggtggc cggcttcgtc gctatgctct ataacacctt
     1741 tctgctcggc ccagccgtgc tctggctgat cgccacggtc gcgctcctgg tccggcacac
     1801 cctccaacaa cccaccccga ccaccgcatc cggcctgccg caagcgctgt ccgccctgcc
     1861 cgccgacagc cggccgccgc gctaaccggc ggccgcccgc cccgattcta acacaagtcc
     1921 aacgcaaacc agcccggcca agattgacaa tagtcttatc aagtcagtat aatttactca
     1981 aagctgatta tgctgatcca taaaacgaca gccggctaac cggaaaaaag gaagcgaagc
     2041 tgtgcaagtt aaagactatt acaagacgtt gggcgtggcg cgcgaggcca cggacaagga
     2101 gatccgctcc gcgtaccgca agctggcgcg caagtatcac cccgacgtaa atccgaataa
     2161 taaagaggcc gaagccaagt tcaaggacat caacgaagcc tatgaagtcc tcagcgacgc
     2221 cgagaagcgc aagaagtacg acaagttcgg ggccgactgg gagcagttcg agaaaatggg
     2281 cggagcaccc accgacttcg acttcagccg ctacggcgcg ccgggcggcg ggcgcgtcta
     2341 caccggcgcg gcccccggcg gcgaaggctt tagcgacttc ttcgagtcgc tgttcggcgg
     2401 catgggcgcc cgcggcggcg tgggccgcga cccgttcggc ggcatgggcg gcatcggcgg
     2461 ctttggccgg gccgtgccgc gccgcggcga ggacttcgag catcccatcg aggtgacgct
     2521 cgaagaagcc ttcaacggca ccagccgccg cctgcaaatg cagacggaag acaactgccc
     2581 cacctgcggc ggcacgggca tggtgaacaa ccggccctgc cccacctgcg gcggcagcgg
     2641 cgtcgtggcg cgcatgaaga ccatcgaggt caacatccca gcgggcgtgc ataccggctc
     2701 gcgcgtgcgg gtgcgcggcg agggtggccc cggctcggaa ggccagcccc gcggcgacct
     2761 gttcctgatc gtcaccgtgg tgccgcaccc gcgcttcgag cgcaagggcg acgacctgag
     2821 cgaggcggtg gccgtgcccc tgtatacgat ggtgctgggc ggcgaagccc gcgtgcccac
     2881 gttgaagggc accaacctgg cgctgaatat cccggcgggc accgccaacg gccgcacctt
     2941 ccggctcaag ggccagggca tgccccacct gggcaacccg caacagcgcg gcgacctgct
     3001 ggtgaaggcc gaggtcgagt tgcccaagca gctcagcggc gaggaaagac aactattcga
     3061 acagttgcaa cgtatacata aggaggccgg atgatgagcg aattcggcca caacgacgag
     3121 ccgcgctacg tgatcagcgt cgcggcgcaa ttgcttcagg ttcacccaca gaccctgcgc
     3181 ctctatgaac gggaa
//
LOCUS       DASUYC010000541         7943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_239281,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000541 DASUYC010000000
VERSION     DASUYC010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 7943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7943
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239281"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1274)
                     /locus_tag="VF276_08875"
     CDS             complement(<1..1274)
                     /locus_tag="VF276_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX6607012.1"
                     /translation="MKAVVMAGGEGSRLRPLTLRRPKPMVPVVDKPCMAHILDLLKKH
                     GVDEVVVTLQYMAETIQEYFGDGSSLGLTIHYSVEESPLGTAGSVKQAEKWLDDTFLL
                     ISGDAVCDFDLTKAVEYHREKGSMATLVLKRMPNPLEYGVVIIDDEGHIKQFQEKPSW
                     GEVFSDTVNTGIYVLDPKVLSYFKAGKVFDFSMNLFPMMLKKGDPMYGYVADGYWCDI
                     GSIPDYMRSTGDLLAGQVDMPPAGKEVAPGVWAEDDVEIAPDAHLHGPIFLACGVKVK
                     EGATIQGPAVIRDYTIVDTRAIVDRSIVWRNCYIGERAEVRGAILCRQVSVKSRAMIF
                     EGVVVGDNTTVGEGAMIQPNVKVWPDKEIEAGATLSTSLIWGAQGRRNLFGRFGVTGV
                     VNVDLTPEFTAKLGAAYGATLNRGAMVAVNRDA"
     gene            1553..1777
                     /locus_tag="VF276_08880"
     CDS             1553..1777
                     /locus_tag="VF276_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607013.1"
                     /translation="MSRYSRRRSRSGSRGAPARLVSNPLIVVALALFVVGLLGTVVHV
                     SSLPIPQDIGIWSLITSNVLLLIGAFSREM"
     gene            1851..2336
                     /locus_tag="VF276_08885"
     CDS             1851..2336
                     /locus_tag="VF276_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607014.1"
                     /translation="MNYQVVYDASQTGIDWLPIFISTILLSGAIIAWRGRHSPYFRGE
                     PWLAGIFLIFALIFSPCVIIGKVNEYITAQTRLHANQTAVVEGRIENFNPGVYPNVLE
                     SFTVNGVRFEYSEFASTPGFHHTSIYGGPMKAGLQVRIHYTNLAGQTVHPTILRLEIA
                     K"
     gene            complement(2434..3846)
                     /locus_tag="VF276_08890"
     CDS             complement(2434..3846)
                     /locus_tag="VF276_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6607015.1"
                     /translation="MSDSSPSRRKTRAPKATSAAPTPAVGSGGKSTPQAAAAAALDPE
                     VLDSAPVENAPDELDENALEPELESAPDITELPEPAEAELVAVAKEDDVEGEELERVI
                     ERTLHMDGMSVDDPVRLYLREIGKTALLKAPDEQALAQRMEAGRTANARLEELGYHSP
                     VPDWAEAASSSTVHVSNFGRNNMQIATVTTNVPVTTVQAVVSPEMTAEEAAAAKAEVM
                     RLLITRDSGIAAKQQLVQANLRLVVSIARRYLGRGLSLLDLIQEGNIGLMRAADKFDY
                     NRGFKFSTYATWWIRQAITRAISDQGRTIRLPVHVSEMVARLRRETHQLQQTLQREPT
                     EEELAKALDISPAKVRRIVEIAKQPVSLESPLDDAEDSFLGDFIEDSTVATPIEEASR
                     QLLREEVRKVLLNLSERERKIVELRYGLVDGKQRTLEEVAQEFRITRERIRQIETKIL
                     RKLRHQRFGGSKLQAYLNDG"
     gene            complement(3900..4292)
                     /locus_tag="VF276_08895"
     CDS             complement(3900..4292)
                     /locus_tag="VF276_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607016.1"
                     /translation="MSEPIPFPSGPNAASDAGMDAEMTEVTLDGAPAAAPSRATHRLP
                     DEPDLVAELSEVLLAPLNVFPPETRAHLRTAGREVALTLTSLAGTVLKGAAIALNVAA
                     ESLKDYTARNSTASASQPARRRVEIEVE"
     gene            complement(4429..5628)
                     /locus_tag="VF276_08900"
     CDS             complement(4429..5628)
                     /locus_tag="VF276_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRC40/GET3/ArsA family transport-energizing
                     ATPase"
                     /protein_id="HEX6607017.1"
                     /translation="MRILLYTGKGGVGKTSVSAATALRLAQQGHRTVVLSTDAAHSLG
                     DSLDTRLGPEPREIAPNLWAHEVEVLHEMDEHWVTLQTYMNALLSSQGLESVVAEEMT
                     VLPGAEELAGLVRIIQHYDSKRFDVVVVDCAPTGETLRLLSFPDAARWWLQKLFPIQR
                     KMAKLLRPVGRMVMPDVPMPQDNIFDSVERLLRQIDRIHELLADPELTSVRIVLNPEK
                     MVVKEAQRVFTYLNLFGYATDMVVCNRVLPAAVHDSYFAGWKQSQERYHQLVVEGFSP
                     LPVLDVPLFDQEVVGLEMLNRMADAIYGDKDPSQVFFTGKTEEIEAQGDGYVLRLPMP
                     FAEKGAVEMTQVGDELIVQVGSYKRNMILPRALATLRASSAKLDGGELRVAFRPFDPV
                     EAPATRR"
     gene            complement(6032..6943)
                     /locus_tag="VF276_08905"
     CDS             complement(6032..6943)
                     /locus_tag="VF276_08905"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6607018.1"
                     /translation="MRAKAEVDTIRQLDDRVESFDGTEIAVRVWEPAEAGRPGSPVAV
                     LHHGVAYYGAAYAGLGRFLAGLGVALCAPDARGHGRSGGARGEMVSGRTMLRDLHAVV
                     CWARRRYPNRPVVLIGESMGGLFALNYAALYATQPHQVAGVLLVAPGLLIHPRQIADF
                     TVVRHTPQARRAAEAHGRAMRAWRAALAGSRDAAWMRARDNDPLVLPGVSGRYMLTII
                     AMSMRCSVTARRCTPPTLIIHGKRDGIVPYQGSLMLYHMLGAEDKALILFPEVWHTMF
                     WDPDTPAVLERIEAWLGAHFPIDSRSG"
     gene            complement(6927..7808)
                     /gene="trmD"
                     /locus_tag="VF276_08910"
     CDS             complement(6927..7808)
                     /gene="trmD"
                     /locus_tag="VF276_08910"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018750847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX6607019.1"
                     /translation="MRVDILTLFPEMFPGPLGASILKRAMQAGHLGVRLHQIRDYTYD
                     RHHTVDDYPYGGGAGMVMKAPPLFEAVETVLTLPPAPPGLYLWHRDGPADPLVAPDML
                     PPDSEAPQPNATDPPAAPPVILMSPAGRVLTQRVAAELAALPRLVLVCGHYEGVDERV
                     REHLITDELSIGDYVLTGGELAALVVVDAVARLVPGVLAEGSADDESHTSGLLEYPQY
                     TRPASFRGWDVPPLLVSGHHARVAEWRRREALRRTVARRPDLLAERTLTKQEQAWLAE
                     LQADPAKEDDAPHAGEG"
BASE COUNT         1286 a         2696 c         2568 g         1393 t
ORIGIN      
        1 gcgtcgcggt tgacggcgac catggcgccg cggttcagcg tggcgccgta ggccgcgccc
       61 agcttggccg tgaactccgg tgtcaggtcc acattgacca cgccggtgac gccgaaacgg
      121 ccaaagaggt tgcgccggcc ctgcgcgccc cagatgaggc tggtcgagag agtggccccg
      181 gcctcgatct ctttatcggg ccagaccttg acgttgggct ggatcatcgc cccctcgccc
      241 accgtggtgt tgtcgccgac gaccacgccc tcaaaaatca tggcgcggct cttgacgctc
      301 acctggcgac acaggatcgc cccgcgcacc tccgcgcgct cgccgatata gcaattgcgc
      361 cagacgatgc tgcggtccac aatcgcgcgg gtgtccacga tggtgtagtc gcggatcacc
      421 gccggtccct ggatcgtggc gccctctttc accttcacgc cgcaggccag gaaaatcggg
      481 ccgtgcagat gcgcgtccgg ggcgatctcc acgtcgtctt cggcccacac gcccggcgcg
      541 acttccttgc cggcgggcgg catgtccacc tgcccggcca gcaggtcgcc cgtcgagcgc
      601 atatagtcgg gaatgctgcc gatgtcgcac cagtagccgt cagccacgta gccgtacatc
      661 gggtcgccct tcttgagcat catcgggaac aggttcatgc tgaagtcgaa gaccttgccc
      721 gccttgaaat agcttaagac cttcgggtcc agcacataga tgccggtgtt gacggtgtcg
      781 gagaatactt cgccccagga cggcttctcc tggaattgct tgatgtggcc ttcgtcgtcg
      841 atgatcacca ccccatactc cagcgggttg ggcatgcgct tgagcaccag ggtcgccatc
      901 gaccccttct cgcggtgata ttccaccgct ttggtcagat cgaaatcgca cacggcgtcg
      961 ccgctgatca gcaggaaggt atcgtccagc cacttctccg cctgcttcac gctgccggcg
     1021 gtgccgagcg gcgattcttc cacgctgtaa tggatggtca ggccgagcga actgccgtcg
     1081 ccgaaatact cctggatggt ctcggccata tactggaggg tgaccactac ttcgtcgacg
     1141 ccgtgcttct tcagcagatc gagaatgtgc gccatgcacg gcttatcgac caccgggacc
     1201 atcggcttgg gccggcgcaa ggtgaggggc cggaggcggg aaccctcccc gccggccatg
     1261 accactgcct tcatcgctgc tcctcctctg tctggtgccg gaacagcgtc cgcgcccggt
     1321 tttgcgcgcg cacctgtccc gattaggggc gggatgtttg agtcggatta tacaacggcc
     1381 cgcgaaccga gtcaaaacgg cagactccgc ctgccctccc agccgccggc cttgccccgg
     1441 cccaggccgg catgcgataa taggaggccg ccgctcaaca atcggttccg ttctcagcac
     1501 ttaccgggcc agggatggcc cgcaggcggc ttaccaggag gaaacatcgc gcatgagtcg
     1561 ctattcgcgc cggcgctcgc ggagcggcag ccgcggcgcg cccgcccggc tggtcagcaa
     1621 cccgctgatc gtcgtggccc tggccctgtt cgtggtcggg ctgctcggca cggtggtcca
     1681 cgtgtccagc ctgcccatcc cgcaggacat cggcatctgg agcctgatta cctccaacgt
     1741 cctgctgctg atcggcgcct tcagccggga gatgtgatag cggcccagcg tgcctcagaa
     1801 cgctgcacaa caaacaacct cagtagcacg tcccttgaag gagccggtcg ttgaactacc
     1861 aggtcgtcta cgatgcatcc caaaccggca ttgattggct acctattttt ataagcacga
     1921 tcttactctc gggcgccatc attgcctggc ggggccggca tagcccatac tttcgtggtg
     1981 agccttggct tgcagggatc tttctgatct ttgctctgat attctctccg tgcgtcatca
     2041 tcgggaaggt gaatgagtat atcaccgcgc agacacgctt acacgcgaac cagactgcgg
     2101 tagtagaagg gcgcattgag aatttcaatc ctggtgtcta tcccaacgtg ttagaaagtt
     2161 ttaccgtcaa tggagttcgt tttgagtatt ccgaattcgc cagcactcca ggattccatc
     2221 acaccagtat ttatggaggg ccaatgaaag cgggactcca ggtgcgtatc cattatacaa
     2281 accttgccgg tcagaccgtg cacccgacga ttctgcggct ggagattgcc aagtaaaacg
     2341 tggccgaaca aagtttgatc aattggagcc aaacaaaagc gggacggctg ttggagccgc
     2401 cccgccaggg gtcgcgttcg atagccgggt gcatcacccg tcgttgaggt aggcttgcag
     2461 tttgctgccg ccgaagcgct ggtggcgcag cttgcgcagg atcttcgtct cgatctggcg
     2521 gatgcgctcg cgggtgatgc ggaactcctg tgccacctct tcgagcgtgc gctgcttgcc
     2581 gtccaccagg ccgtagcgca attccacgat cttgcgctcg cgctccgaca ggttgagcag
     2641 caccttgcgt acttcctcgc gcagcaactg gcgcgaggct tcctcgatgg gcgtcgccac
     2701 ggtcgagtct tcgatgaagt cgcccaggaa gctgtcctcg gcgtcgtcca gcggcgactc
     2761 cagggacacc ggctgcttgg cgatctccac gatgcgccgc accttggccg ggctgatgtc
     2821 gagcgccttg gcgagttcct cctcggtcgg ctcgcgttgt aacgtctgct gcaactggtg
     2881 ggtctcgcgg cgcaggcggg ccaccatctc gctcacgtgg accggcaggc ggatcgtgcg
     2941 cccttggtcg ctgatggcgc gggtgatcgc ctggcggatc caccaggtgg cataggtcga
     3001 gaacttgaag ccgcggttat agtcgaactt gtcggcggcg cgcatcaggc cgatattgcc
     3061 ttcctggatc aggtcgagca acgagaggcc gcggcccaga tagcgccggg cgatgctcac
     3121 caccaggcgc aggttggcct gcaccagttg ctgcttggcg gcgataccgc tgtcgcgggt
     3181 aatcagcagg cgcatcacct cggccttggc tgccgccgcc tcttcggccg tcatctccgg
     3241 gctgacgacg gcctgcaccg tcgtgaccgg cacgttggtg gtcacggtgg cgatctgcat
     3301 gttgttgcgg ccaaaattgg agacatggac cgtgctcgac gaggcggcct ccgcccaatc
     3361 gggcaccggc gaatggtagc ccagttcttc cagccgggcg ttcgccgtgc gcccggcctc
     3421 catgcgctgg gccagggcct gttcgtcggg cgccttgagc agcgccgtct tgccgatttc
     3481 gcgcagatag agccgcaccg ggtcgtcgac gctcatgccg tccatgtgca gcgtccgctc
     3541 gatcacgcgc tccagttctt cgccctcgac atcgtcctcc ttcgccacgg cgaccagttc
     3601 ggcctccgcg ggttcgggca gctcggtgat atcgggcgcg gattccagtt cgggttccag
     3661 cgcgttctcg tcgagttcgt cgggcgcgtt ctcgaccggt gcgctatcga gcacctcggg
     3721 gtccagagcc gcggcggcgg cggcctgggg cgtgctcttg cccccgctcc cgactgccgg
     3781 agtcggggcg gcggaggtcg ctttaggggc gcgtgttttg cgccgcgagg gtgatgagtc
     3841 ggacatggcg aactcctggg gatttgcaaa cggatgccgg cacacccgcg ccgggcgtgt
     3901 tattcgactt cgatttcgac gcggcggcgc gcgggctgcg aggcgctcgc cgtgctgttg
     3961 cgggcggtgt aatccttgag cgactccgcc gccacgttga gggcgatggc cgcgcccttg
     4021 agcacggtgc cggccagact ggtcagcgtg agcgcaactt cgcggcccgc cgtgcgcagg
     4081 tgggcgcggg tttccggcgg aaagacgttc agcggcgcca gcaatacttc actaagctcg
     4141 gccacgagat ccggctcgtc gggcaaacgg tgcgtggccc gcgacggcgc cgcggcaggc
     4201 gccccatcga gggtcacttc agtcatctcg gcatccatgc cggcatcgga cgcagcgttg
     4261 ggtccactgg ggaaaggaat gggttcgctc atggtcgtgt ctcctcgggc ggcaccggca
     4321 ccgggaccgg ttaccggggt aggtgcccgc cgggtatggt aactatacag gatcgtcccg
     4381 cgtttgtcca gcgcccgctg caggccggcc gccgaacccg ccggccctct agcgccgggt
     4441 cgccggggcc tccaccgggt caaacgggcg gaaggcgaca cgcaactcgc ctccgtccag
     4501 tttcgcgctg ctggcgcgca aagtcgccag ggcacgcggc aaaatcatat tccgcttata
     4561 actgcccacc tggacgatca actcatcgcc cacctgggtc atctcgacgg cgcccttctc
     4621 ggcgaagggc atcggcaggc gcagcacata gccgtcgccc tgcgcctcga tctcctcggt
     4681 cttgccggtg aagaagacct ggctggggtc cttgtcgcca tagatggcgt cggccatgcg
     4741 attcagcatc tccaggccga ccacttcctg gtcgaacagc ggcacatcga gcaccggcag
     4801 cggcgaaaag ccctcgacca ccaactggtg atagcgctcc tgcgactgct tccacccggc
     4861 gaaatagctg tcgtggaccg ccgccggcaa cacgcggttg cataccacca tgtcggtcgc
     4921 gtagccgaac aggttcaagt aggtgaagac gcgctgcgcc tccttgacca ccatcttctc
     4981 cggattgagg acaatgcgga ccgaggtcag ttccgggtcg gccagcaact cgtggatgcg
     5041 gtcgatctgg cgcagcaggc gctccaccga gtcgaagatg ttgtcctgcg gcatgggcac
     5101 gtcgggcatg accatgcggc ccaccgggcg cagcagtttc gccatcttgc gctggatcgg
     5161 gaacagcttc tgcaaccacc agcgcgccgc gtcggggaac gacagcaggc gcagcgtctc
     5221 gcccgtgggc gcgcagtcga ccacgaccac gtcgaaccgc ttgctgtcgt agtgctggat
     5281 gatgcgcacc agcccggcca gctcctcggc ccccggcagg acggtcatct cctcggccac
     5341 cacgctctcc aggccctgcg aggacagcag cgcgttcata taggtttgca gcgtgaccca
     5401 gtgctcatcc atttcatgca gcacttcgac ctcgtgcgcc cagaggttgg gcgcgatctc
     5461 gcgaggctcg gggccgaggc gggtgtccag cgaatcgccg aggctatggg cggcgtcggt
     5521 gctcagcacc acggtgcggt ggccctgctg cgccaggcgc agcgcggtgg ccgcggagac
     5581 ggacgttttg ccgactcctc ctttgccggt ataaagcaga atacgcacga aatgtccttt
     5641 ccaggcaggc gacgcaaaaa ccaatctgcg ccgattctac cattgtcggc gaagtgtcgc
     5701 aagttacggg cgcgcgccgc gggttagccg ccgctcatac ccccgcgcag ccgcgcatca
     5761 cgcccggacc tgacctacta taaataaact tttgtgcaac gggcggctgc gcgattcaat
     5821 gtgcgaatcg atctacgacc gcgaccgtaa tccggttgcg ccgtagcacc accggcatct
     5881 gcgctgtagg ggccggtttg aaaccggccc gcgtcgccca cccgcatcta ccgatataga
     5941 actgccggca tctgcgctgt aggggccggt ttgaaaccgg cccgcgccgc ccacccgcgc
     6001 caccgcaatg ggcgccccga ccgggcaccg tttacccgct ccggctatcg atggggaaat
     6061 gggcgcccag ccacgcctcg atccgctcca ggaccgccgg agtgtcgggg tcccagaaca
     6121 tggtgtgcca aacttccggg aacaggatga gcgccttgtc ctccgcgccg agcatatggt
     6181 agagcatcag cgacccctgg tagggcacga tgccgtcgcg cttgccgtgg atgatcagcg
     6241 tgggcggcgt gcagcggcgg gccgtgacag agcagcgcat actcatggcg ataatcgtca
     6301 gcatataacg gccactgacg ccgggcagca cgagcgggtc gttatcgcgg gcccgcatcc
     6361 aggcggcgtc gcgcgacccg gccagcgccg cgcgccaggc gcgcatggcc cgcccgtgcg
     6421 cctcggcggc gcggcgcgcc tgcggcgtgt gccgcaccac ggtgaaatcg gcgatctggc
     6481 gcggatggat cagcagcccc ggcgcgacca gcagcacccc cgccacctgg tggggctgcg
     6541 tggcatacag cgccgcgtag ttgagcgcga acagcccgcc catgctctcg ccgatcagca
     6601 cgaccgggcg gttgggatag cgccgccgcg cccagcagac cacggcatgg agatcgcgca
     6661 gcatggtccg cccgctgacc atctcgccgc gggcgccgcc ggaccgtccg tggccgcgcg
     6721 catcgggcgc gcagagcgcc acgcccagcc cggccaggaa gcggcccagc cccgcgtagg
     6781 ccgcgccgta gtaggcgacg ccgtgatgca ggacggcgac cggactgccg ggccgcccgg
     6841 cctccgccgg ctcccagacg cgcacggcga tctcggtgcc gtcgaacgac tcgacccggt
     6901 cgtccaattg ccggatggtg tctacctcag ccttcgcccg catgcggcgc atcgtcctcc
     6961 tttgccggat cggcctgcaa ctcggccagc caggcttgct cttgcttcgt cagtgtgcgc
     7021 tcggccagca gatcggggcg gcgcgcgacc gtgcgccgca acgcctcgcg gcgccgccac
     7081 tcggccaccc gcgcatggtg cccgctgacc agcagcggcg gcacatccca gccgcgaaag
     7141 ctcgccgggc gcgtgtactg cggatactcc agcaggcccg acgtgtgcga ctcgtcatcc
     7201 gccgaacctt cggccagcac gcccggcacc agccgggcca ccgcatcgac caccaccagc
     7261 gcggccagtt cccccccggt gagcacgtag tcgccgatcg acagttcatc ggtgatcagg
     7321 tgttcgcgca cccgctcgtc cacgccctcg taatgaccgc agaccagcac caggcgcggc
     7381 agcgccgcca gttcggcggc cacccgctgg gtcagcaccc ggcccgccgg gctcatcagg
     7441 atcacgggcg gcgccgcagg cggatctgtt gcgttcggtt gcggtgcttc agagtccgga
     7501 ggaagcatat cgggggccac cagtgggtca gccgggccgt cccgatgcca caggtaaagg
     7561 ccgggcggcg ccggtggcaa ggtcagcact gtctcgacgg cttcaaacag cggcggcgcc
     7621 ttcatcacca tgcccgcgcc gccaccgtag gggtaatcgt ccaccgtgtg gtgccggtcg
     7681 taggtgtaat cgcggatctg gtgcaggcgc acccccaggt ggcccgcctg catggcgcgc
     7741 ttgaggatgc tggcccccag cgggccgggg aacatttcgg gaaaaagcgt caggatgtca
     7801 acacgcatag gcggggccgg gcgggctaaa tatcgtcaaa caacgggtcg aggtcgccgg
     7861 acgcggcggg tttgcgctgc ggcccggtag tgggcggcag cccgcggcga cccggcccgc
     7921 cgagtggccc ggctagcgac ccc
//
LOCUS       DASUYC010000542         2933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1193579, whole genome shotgun sequence.
ACCESSION   DASUYC010000542 DASUYC010000000
VERSION     DASUYC010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193579"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(225..1451)
                     /locus_tag="VF276_08915"
     CDS             complement(225..1451)
                     /locus_tag="VF276_08915"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEX6607020.1"
                     /translation="MDELLNTAPCGFVEFADDGTIVLVNDTLASLLGYAPGDLPGRGM
                     NVLLTPGSRVFHQTHFFPLLKMQGRVDEIYLSLRSRQGADIPVLANAVRRERAGMVVN
                     DCVVVPMYQRRRYEDELLQAKKAAEGVTAQLAEANRQMAVMLLEVEKSHAELQHTETE
                     LVTANMRLQELDRLKDELLAVTSHDLRTPITTIRMATDILLDEKRAARVDHRRYLETI
                     SQATKRLLVLSNDLSDLARINTGELHLELRPVLLSEVARAAVRELTVTAAAKSITMSV
                     DGAGEEPPVSGDYNRLYQVVSNLLGNAVKFTPTGGQVHIRVQPEPGQVALQVQDTGIG
                     IPAGQVAEMFQHFKHASRPGTAGERGTGLGLTIVRRLVELHGGQIAVESEEGRGTTFT
                     IRLPVAGPAPARNGAG"
     gene            complement(1477..2289)
                     /locus_tag="VF276_08920"
     CDS             complement(1477..2289)
                     /locus_tag="VF276_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015210267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6607021.1"
                     /translation="MIQDILTRNNVRVFGNGTQPMMFAHGFGCDQNMWRFVAPAFADD
                     YRVVLFDYVGSGRSQRTAYDPERYGDLGGYAQDVLDICAALDLHDVIFVGHSVSSMIG
                     LLAATRDPAHFARLIMIGPSPRYINDPPEYVGGFERADIEGLLDLMDKNYLGWANFLA
                     PLIMQNPDHPELAGELEQSFCSTDPVTARRFAEATFFADNRRDLARSPVPALILQCVE
                     DAIAPREVGEYVHRHLPGSTLYMMQATGHCPHLSHPDETVSAIKAYLVAGAR"
     gene            complement(2339..>2933)
                     /locus_tag="VF276_08925"
     CDS             complement(2339..>2933)
                     /locus_tag="VF276_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6607022.1"
                     /translation="IDGLAGRLRADFGEPCLDIVIHSLANGPEVKKPLIETSRQGYLS
                     AVSVSSYSLVSMVRQMAPLMRPGGTFLCLSYMAGERIIPGYGGGMSSAKAALESDVRV
                     LAYEAGRRYGVRVNCISAGPLASRAASAIGMIDRMVEFVAANAPLPEPMAASEVGNAA
                     AFLSSPLAAGITGTMLYVDKGYHAMGMPATEDPRGGS"
BASE COUNT          448 a          972 c         1002 g          511 t
ORIGIN      
        1 aactggacgc gctgctccgg gatggccgga tcgggatgca ggtcataata ctggccgcgc
       61 aatttagcga catcgatcag catgtccggc ggcgccggtt tcccggccag gggactgatg
      121 tgctcttcca cggtaatcct ctccctttct ccagccatga gtcgtccctg gccctgtatc
      181 agattccgtg cccggcgttt cgccggtgcc cgcctcgcgc tgccctatcc ggccccattg
      241 cgggcggggg ccggcccggc caccggcagc cggatggtga aggtcgtgcc acggccctcc
      301 tccgactcga cggcgatctg cccgccgtgc agctccacca ggcggcgcac aatggtcagg
      361 cccaggccgg tgccgcgctc gccggcggtg ccggggcggc tggcgtgctt gaagtgctgg
      421 aacatctcgg ccacctgccc cgccgggatg ccgatgcccg tatcctgcac ctgcaacgcc
      481 acctggcccg gctcgggttg cacgcggatg tggacctggc ccccggttgg ggtgaatttg
      541 acggcgttgc cgagcaggtt gctgaccacc tggtagagcc gattataatc gccggacacg
      601 ggcggttcct cgccggcccc gtccacgctc atcgtgatcg acttggccgc cgcggtgacg
      661 gtcagttcgc gcacggcggc gcgggccacc tcgctcagca agacgggccg tagctccagg
      721 tgcaactcgc cggtgttgat ccgggccagg tccgacaggt cgttgctgag gaccagcagg
      781 cgcttcgtcg cctgggaaat cgtctccagg tagcgccggt gatccacacg cgccgcgcgt
      841 ttctcgtcca gcaggatgtc ggtcgccatg cggatggtcg tgatcggcgt gcgcaggtcg
      901 tgcgaggtga cggccaggag ttcgtccttg aggcggtcca actcctgcag gcgcatattg
      961 gcggtcacca gttcggtctc ggtatgctgc aattcggcgt gcgatttttc gacctccagc
     1021 aacatgacgg ccatttgccg gttcgcctcg gccagttgcg cggtgactcc ctccgccgcc
     1081 tttttcgctt gcagcaattc gtcctcgtag cggcggcgct ggtacatcgg cacgaccacg
     1141 cagtcgttga cgaccatgcc cgcgcgttcg cgccggacag cgttggcgag cacggggata
     1201 tccgccccct ggcgcgaccg caacgagaga tagatctcgt cgactcggcc ctgcattttc
     1261 aagagcggga agaagtgcgt ctggtggaag acgcgactgc cgggcgtgag caacacgttc
     1321 atcccgcgac ccggcaggtc gccgggcgcg tagccgagca gggacgccag ggtgtcattg
     1381 accagcacga tggtgccgtc atcggcgaac tccacgaagc cgcagggcgc agtgttgagc
     1441 agttcgtcca ttaggcgcgg cgctccttcc cccggctcat ctcgcgcccg ccaccagata
     1501 ggccttgatg gcgctgacgg tctcgtcggg atggctgaga tgggggcaat ggccggtggc
     1561 ctgcatcatg tacaaggtgc tgccgggcag gtgccggtgg acatattcgc ctacctcgcg
     1621 cggcgcgatg gcgtcttcga cacattggag gatcagcgcg ggcaccggcg accgggccag
     1681 atcgcgccgg ttgtcggcga agaaggtggc ctcggcgaag cggcgggccg tgaccgggtc
     1741 ggtcgagcag aagctttgct ccagttcgcc cgccagttcc gggtggtccg ggttctgcat
     1801 gatcaggggc gccaggaagt tggcccagcc caggtagttt ttatccatca ggtcgagcag
     1861 gccctcgatg tcggcgcgct cgaagccgcc cacgtactca ggcgggtcgt taatgtagcg
     1921 cggcgagggg ccgatcatga tcagccgggc gaagtgggcc gggtcgcggg tggctgccag
     1981 caagccgatc atgctgctca ccgaatggcc gacgaagatc acgtcgtgca gatccagcgc
     2041 cgcgcagatg tcgagcacat cctgggcgta gccgccgaga tcgccgtacc gctccgggtc
     2101 gtaggcggta cgctgcgagc ggcccgaccc cacatagtca aagaggacga cccggtagtc
     2161 gtccgcgaag gcgggcgcca cgaaccgcca catattctga tcacagccga agccatgggc
     2221 aaacatcatc ggctgggtgc cgttgccaaa gacgcgcacg ttgttgcgcg tgaggatgtc
     2281 ctgtatcatg cctttgcttt ccgggcgcgg ggtggcgccg tcgtgtcccc tgtcgatctt
     2341 agctgccgcc gcgcggatcc tcggtcgcgg gcatgcccat ggcgtggtag cccttgtcca
     2401 cgtagagcat cgtgccggtg ataccggcgg ccagcgggct gctgaggaac gcggcggcgt
     2461 tgcctacctc ggacgcagcc atcggctcag gcaggggggc gttggcggcc acgaactcga
     2521 ccatgcggtc gatcatgccg atggcgctgg ccgcccgcga cgccagcggc ccggcggaga
     2581 tacagttgac gcgcacgccg tagcgccgcc ccgcttcgta ggccagcacc cgtacgtcgc
     2641 tctccagcgc ggccttggcc gacgacatgc cgccgccgta gccggggatg atgcgctcgc
     2701 cggccatgta gctgaggcag aggaaggtgc cgccgggccg catcaacggc gccatctggc
     2761 gcaccatcga caccagcgag tagctgctga cgctcaccgc cgacaaataa ccctggcggc
     2821 tggtctcgat cagcggcttt ttcacttcgg gcccgttcgc cagcgagtgg atgacgatgt
     2881 ccaggcacgg ctcgccgaag tcggcccgca ggcgcccggc gagcccgtcg atc
//
LOCUS       DASUYC010000543         2109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_239950,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000543 DASUYC010000000
VERSION     DASUYC010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239950"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1884)
                     /locus_tag="VF276_08930"
     CDS             complement(<1..1884)
                     /locus_tag="VF276_08930"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HEX6607023.1"
                     /translation="MKTKTLHMIGNAHLDPVWLWQWQEGFQEAKATFRSALDRLREYP
                     DFIFTSSSAAIYEWIEHNDPEMFAEIQARVAEGRWQICGGWWIQPDCNLPSGESFVRQ
                     GLYGQRYFQEKLGVTARTGYNVDSFGHNGMLPQILRKSGMDAYIFMRPSPQEMGLAGP
                     LFWWEADDGSRVLTYRIPYEYASPGKDIETHIRRCAQEIKAPFTEGMCFYGVGNHGGG
                     PTKENLECIRRLNEAPNLPRLVMSGPNAFFEHVRAAGDLPIPVVHTDLQHHASGCYAA
                     HSGVKRWNRRAENALVAAEKWAGLAHWLTGLPYPAEFAHAWKLMLFNQFHDILAGTSI
                     EAAYEDARDNFGEALSIAARAANHAMQSISWRIDIAPEEGMKPIVVFNPHAWQSKVNV
                     ELEWGRLAEGSTLLDDADQPVAVQTVQSAATAGGRSRLSFVSDLPPLGYRVYRVVLRP
                     APETPPGAEPPAGNVALQASDTAMENAWFKLEFDPETGYIRSLTDKQRGVELFRAPGA
                     RPVVMNDPSDTWSHNVLRFDNVAGEFRATAVKLVANGPVKAIIRVLSSYGSSQLIQDF
                     AMYRDLPQIEVRAMVDWNERFQLLKLQFPLNLNFMRATYEIPYGHIQRPVNGEEEPGQ
                     SWLD"
BASE COUNT          334 a          673 c          702 g          400 t
ORIGIN      
        1 gtcgagccag ctctggcccg gctcctcctc cccgttgacc gggcgctgga tgtgcccata
       61 ggggatctcg taggtggcgc gcatgaagtt gaggttgagc gggaattgca gcttcagtaa
      121 ctggaatcgc tcgttccaat ccaccatcgc ccgcacctcg atctgcggca ggtcgcggta
      181 catggcaaag tcctggatca gttgcgacga gccgtagctg ctgagcacgc ggataatcgc
      241 cttgaccggc ccgttggcga ccagcttgac ggcggtggcc cggaactccc cggccacgtt
      301 gtcgaagcgc agcacgttgt ggctccaggt gtcgctgggg tcgttcatca ccacggggcg
      361 cgcgccgggg gcgcggaaca gttccacgcc gcgctgcttg tcggtgaggc tgcggatata
      421 gcccgtctcc gggtcgaact ccagcttgaa ccaggcgttc tccatcgcgg tgtcgctcgc
      481 ctggagagcg acattcccgg cgggcggttc ggcgccgggc ggggtttccg gcgcggggcg
      541 cagcaccacg cggtagacgc gatagcccag cggcggcaga tcgctgacga agctgagccg
      601 gctgcgcccg cccgccgtgg ccgccgactg caccgtttgc accgcgacgg gctggtccgc
      661 gtcgtcgagc agcgtgctgc cctcggccag ccgcccccac tccagttcga cgttgacctt
      721 gctctgccag gcgtgcgggt tgaagacgac gatgggcttc atgccctcct ccggcgcgat
      781 gtcgatccgc caggagatcg actgcatggc gtggttggcg gcgcgcgccg cgatggacag
      841 ggcttcgccg aagttgtcgc gcgcatcctc ataggccgcc tcgatgctgg tgccggccag
      901 gatgtcgtgg aactggttga acagcatcag cttccaggcg tgcgcgaact cggcagggta
      961 gggcaggccg gtgagccagt gggccagccc ggcccatttc tcggcggcca ccagcgcgtt
     1021 ttcggcacgg cggttccagc gtttcacccc ggagtgcgcg gcatagcagc cgctggcgtg
     1081 atgctgtaaa tcggtgtgta cgaccgggat gggcaggtcg ccggcggcgc gcacatgctc
     1141 gaagaaggcg ttggggccgc tcatcaccag ccgcggcagg ttcggggctt cgtttaagcg
     1201 ccggatacat tccaggttct ctttggtcgg gccgccgccg tggttgccca cgccgtagaa
     1261 gcacatgccc tcagtgaacg gtgccttgat ctcctgggcg cagcgccgga tatgcgtctc
     1321 gatgtccttg ccgggcgagg cgtattcgta ggggatgcgg taggtcagca cgcgcgagcc
     1381 gtcgtcggcc tcccaccaga acaacggccc ggccagcccc atctcctgcg gcgacgggcg
     1441 catgaagatg taggcgtcca tgccgctctt gcgcaggatc tgcggcaaca tgccgttgtg
     1501 cccgaagctg tccacgttgt agccggtgcg cgccgtcacg cccagcttct cctgaaaata
     1561 gcgctggccg tacagcccct ggcgcacgaa cgactcgccg gaaggcagat tgcagtcggg
     1621 ctggatccac cagccgccgc agatctgcca gcgcccctcg gccacgcggg cctggatttc
     1681 ggcgaacatc tccgggtcgt tgtgctcgat ccactcgtag atggcggcgg aactggacgt
     1741 gaagatgaaa tcgggatact cccgcaggcg gtccagcgcc gagcggaagg tggctttggc
     1801 ctcctggaaa ccctcttgcc actgccaaag ccacacggga tcgaggtggg cgttgccgat
     1861 catgtgcagt gttttcgttt tcaacctggg accctccgta ggcataatag aacagggcga
     1921 ttgtgccgct attgaggcat ccgccagatc tgtgccgccc tgcgcggcga tcatgcgcat
     1981 cgcctggcat ccattcatca atgcaccgcg aagaaacgaa gaacgcgaag attctttata
     2041 ttcgtcttat tcttcgcgtc cttcgttcct tcgcggtgcg taatcgtcgt ttatgatcga
     2101 atcgcaggg
//
LOCUS       DASUYC010000544         2439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1143770, whole genome shotgun sequence.
ACCESSION   DASUYC010000544 DASUYC010000000
VERSION     DASUYC010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143770"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..264
                     /locus_tag="VF276_08935"
     CDS             118..264
                     /locus_tag="VF276_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607024.1"
                     /translation="MPRDNTLLSVGDAAPSFTLPDAEGTPHTPVAAAGAGGKVALFFF
                     RGIW"
     gene            277..621
                     /locus_tag="VF276_08940"
                     /pseudo
     CDS             277..621
                     /locus_tag="VF276_08940"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
     gene            1100..2257
                     /locus_tag="VF276_08945"
     CDS             1100..2257
                     /locus_tag="VF276_08945"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6607025.1"
                     /translation="MNFTHTPDVEMVVQTVRDYVEKSLLPQEMEVEHQARIPAATLRE
                     MADLGFFALPFGEDDGGIGLGFTGYTLAMEQLGRANAAYAIALSASSGLAGETLALAG
                     TPDQRARWLAPLAAGAVLGAWGLVEEGTTSTGQGLRTTATRTGDSYRLDGRKSWVING
                     PDAGLYVIFAQLAGEGESGTAAFVVEQGTAGLTTGPAQTELGLHGVGICEVRLANCVV
                     PAANRLAGVAPDGSADPGLALAGRVLSRYGVTLGAIAVGGAARLLDASLDFALQRRQF
                     GQPVAAFGAVQNMLADSATEIFAAQQAVYRAAWGIEAGRDEPRLAAQAKLFATEMYYR
                     VADRAMQVHGGMGFMKELWIERGYRDARMFRLLGVSSEDLRTALAHLLGCP"
BASE COUNT          382 a          799 c          846 g          412 t
ORIGIN      
        1 ccccctgccc ccttcccggc gccgggaagg gggagatcgg ttatgtacgc cacgcgattg
       61 acagcgcagg aagatgatcg ttatactgcg agaaattccc actgaagagg taggcatatg
      121 ccgcgggata atacattgct atcggtgggc gacgccgcgc cctcgtttac cctaccggat
      181 gccgagggta ctccgcacac gccggtcgcg gctgccggcg cgggcggcaa agtcgcgctg
      241 tttttcttcc gcggcatctg gtgaccgagt tgccggcggc agatgtcgca gttgcgacaa
      301 cactacgacg ccctgaccgg ggccgggtgg gcgatctacg gcatcacgcc ggggcgcccc
      361 gacgccgtgg ccgactacac gcgccaggag cagattcgct atcccgtgct gatcgacgcc
      421 gaccgcgcgg tcagcaagct ctatggcgtt ttcacgccct tcagccatga cggcttcaac
      481 gtgcccacgg tcaatatgcc gcataactcg accttcttga tcgacagcgg cgggcggatt
      541 cgctatatcc acgtcggcgc gcgcagcacc gacctgcccg acgaaagcgc cctgctggcg
      601 gccatggacc aggtgggctg agcccgcgcc gggaggctca agcctcccgc tacctttacg
      661 aagcccgcct gcgcaggctc gtgcaggcgc aagcgaccgc tacgaagccc gcctgcgcgg
      721 gctcgtgtag tcgtaatcgg tcgcttgtgt gtagctgcgc gattcatcgc gccgtcctcg
      781 ttggctttta gcgtgtgcgg tcccactagg ttcttgcaag ctgtactagt ggcactcagt
      841 accgcctgac ggctgacggc tgacggctgt tcgcgcagcg gtgcgtagca cacggctgac
      901 cgctaaatcc cacgttgacg attccggccc ttttatgtta cagtgagggg caagatcggg
      961 ccgggggccc ggtcgtggat attaaacaac gaggtttgat gtccggtcgt ggccttgtac
     1021 gtcccatccc tcccgcgcac ggtcgcggcg gcgggccacg cggtcctcac acctccccat
     1081 ccccaggcat ggacgaccta tgaacttcac acacacgccg gatgtcgaaa tggtcgtgca
     1141 gacggtgcgc gactacgtcg aaaaaagcct cctgccgcaa gagatggagg tggaacacca
     1201 ggcgcgcatc cccgcggcca cgctgcgcga gatggcggac ctgggcttct tcgccctgcc
     1261 cttcggggag gacgacgggg gcatcggcct gggcttcacc ggctacacgc tggcgatgga
     1321 gcaactgggc cgcgccaacg ccgcctacgc catcgcgctg agcgcgagca gcgggctggc
     1381 gggcgaaacg ctggccctgg ccggcacacc ggatcagcgc gcgcgctggc tggcgcccct
     1441 ggccgcgggc gcggtgctcg gcgcgtgggg gctggtcgag gaaggcacca ccagcaccgg
     1501 gcagggtctg cgcaccaccg ccacccgcac cggcgactcc taccggctcg atggccgcaa
     1561 aagctgggtg atcaacggcc ccgacgccgg cctctacgtc atcttcgccc aactggcggg
     1621 cgagggcgag agcggcacgg cggcgtttgt cgtggagcag ggcacggcgg gcctgaccac
     1681 cggcccggcc cagaccgagt tggggctgca cggcgtgggc atctgcgagg tgcgcctggc
     1741 gaactgcgtg gtcccggcgg ccaaccggct ggcgggcgtg gcgcccgacg gatcggcgga
     1801 ccctggtctg gccctggccg ggcgcgtgct gagccgctac ggcgtcaccc tcggcgcgat
     1861 tgccgtgggc ggcgcggcgc ggctgctcga cgcctcgctc gacttcgcct tgcagcgccg
     1921 gcagttcggc cagccggtcg cggcgttcgg cgcggtgcag aacatgctgg ccgacagcgc
     1981 gaccgagatc ttcgccgccc agcaagccgt ctaccgggcg gcctggggca tcgaggcggg
     2041 gcgcgatgag ccgcgcctgg cggcgcaggc caagctgttc gccaccgaga tgtactatcg
     2101 cgtggccgac cgcgccatgc aggtccacgg cggcatgggc ttcatgaagg aactgtggat
     2161 cgagcgcggc taccgcgacg cccgcatgtt ccgcctgctc ggcgtgagca gcgaagacct
     2221 gcgcacggcc ctcgcccacc tgctcggctg cccttgacga gtgctgagtg ctgagtgctg
     2281 agtacgatga gttagtacga gtgcttggtg ctggaggtgt ttggcatcgg ccacggttag
     2341 ggctgggggt tattggtgta gatgaatcag tagttacgga gtttgtgagt gctgatcagg
     2401 taggtgcaag tacgtgtatt cgccacgtac ttgggtgag
//
LOCUS       DASUYC010000545         2451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1376854, whole genome shotgun sequence.
ACCESSION   DASUYC010000545 DASUYC010000000
VERSION     DASUYC010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1376854"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2213)
                     /locus_tag="VF276_08950"
     CDS             complement(<1..2213)
                     /locus_tag="VF276_08950"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6607026.1"
                     /translation="MDSRRAAAGQLFFFNFNDTSLTPDVERFVEQTRPGGVILFGHNL
                     RSAGQVRDLNRQLQALARRLDLPPFIIAVDDEGGRVTRMPNDMRALTAPSPMAQAAAG
                     GPPVAAACAAATARRLRRLGFTLDFAPVADVNNNPLNPIIGSRAFGGDPAVVGDYVVA
                     AVTAFLEGGIAPCVKHFPGHGDTTVDSHLDLPVVHKTLDELAAVELVPFARAIAAGVP
                     AVMTAHIVYPEADPSGRPATLSPQFMQQLLRERLGFDGLLFTDSLSMLAILARYDLAA
                     ATRWALEAGACLLAPNGGFDIQMTCYDALIRAAEDGAFDLEAATARVAAHKARFVEPL
                     LPALAAPLDEARAAEEIADEADALAAVARRSITLLRNRDGVLPLDPALARRPLLVDFD
                     PIIISRVEGAWQPGPVLQAALESRLPGLRTWARNAPYSVADAEELLGLAADADLLLIV
                     TRNAARHAGQAAVAGHLLDLGKPSVVIAAREPYDLMALPAAPAYAVTYSDPPTSLQAL
                     VDLLFGAYRPAGRLPVAIPDLYPVGAGLTDFHDLTRMEGVRVTTVPTEHSTVDFVPYT
                     PEQMPALLEIWDAATEGRLPLSPALWRQRVDEDPLFSPADCLILPAEDGALAGFAITR
                     ILPEWELEANPDLRRHQGPGHLMALAVHPRYTRHGIGSRLLAAAEARLRDQGATSITL
                     QGPPNHFVPGLPVASDSLPFWERRGYTVDHISADLRRRLDDWESPPPPPAVAAGD"
     gene            complement(2206..>2451)
                     /locus_tag="VF276_08955"
     CDS             complement(2206..>2451)
                     /locus_tag="VF276_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607027.1"
                     /translation="VEAANELTIMALATAGQLHFPGPVPLALVGGLLVASPALRGAVV
                     RRIRRRRPVAPVVTVTDPALAAAQALARPAGEGATYG"
BASE COUNT          351 a          848 c          947 g          305 t
ORIGIN      
        1 aagtcgcccg cggccacggc gggcgggggc ggcggcgact cccagtcgtc gagccggcgg
       61 cgcaggtcgg cggagatgtg gtcgacggtg tagccgcgcc gctcccagaa gggcagcgag
      121 tcgctcgcca cgggcaggcc gggcacgaag tggttcggcg gcccttgcag ggtgatcgac
      181 gtggcgccct ggtcgcgcag gcgggcttcg gcggcggcca acaggcggct gccgatgccg
      241 tggcgcgtgt agcgcgggtg gacggccagc gccatgaggt gccccggccc ctggtgccgc
      301 cgcaggtcgg ggttggcctc cagttcccac tcgggcagga tgcgcgtaat ggcgaagccg
      361 gccagcgcgc cgtcctcggc gggcaggatg aggcaatcgg cgggcgagaa cagcggatcc
      421 tcgtccaccc gctggcgcca gagggccggg ctcagcggca gccggccctc cgtcgcggcg
      481 tcccagatct ccagcagcgc gggcatctgc tccggcgtgt agggcacaaa atcgacagtc
      541 gagtgttcgg tcggaacggt ggtcacgcgc actccctcca tacgagtcag atcatgaaaa
      601 tcggtcagcc cggcgcccac cgggtacagg tccgggatgg cgaccggcag ccgccccgcg
      661 ggccggtatg cgccgaacag cagatcgacg agcgcttgca ggctggtggg cgggtcgctg
      721 taggtgacgg cgtaggccgg ggcggcgggc agggccatga ggtcatacgg ctcgcgggcc
      781 gcgataacca cgctgggctt gccgaggtcg agcaggtgcc cggcgactgc ggcctggccg
      841 gcatggcgcg cggcgttgcg ggtgacgatc agcagcagat cggcgtcggc ggccaggccc
      901 agcagttcct ccgcgtcggc cacgctgtag ggcgcgttgc gcgcccacgt ccgcaggccg
      961 ggcaggcgag attcgagcgc ggcctgcaac accggccccg gctgccaggc gccctcgacc
     1021 cggctgataa tgatcgggtc gaaatcgacc agcagcgggc gccgggccaa ggcggggtcc
     1081 aggggcagca cgccgtcccg gttgcgcagc agcgtgatcg agcgccgcgc caccgcagcc
     1141 agggcatcgg cttcgtccgc gatttcctcg gcggcccgcg cctcgtcgag cggggcggcc
     1201 agggcgggca gcagcggctc cacaaagcgc gctttgtgcg cggccacccg cgccgtcgcc
     1261 gcttccaggt cgaaggcccc atcctccgcc gcgcggatca gggcgtcgta gcaggtcatc
     1321 tggatgtcaa agccgccgtt gggcgccagc aggcaggcgc ccgcctccag cgcccagcgc
     1381 gtcgccgcag cgaggtcgta gcgcgccaga atagcgagca tgctcaacga gtcggtgaag
     1441 agcaggccgt cgaagcccag ccgctcgcgc agcagttgct gcataaactg gggcgacaga
     1501 gtggccgggc gcccgctggg gtcagcctcc gggtagacga tgtgcgccgt catgaccgcg
     1561 ggcaccccgg cggcgatggc gcgggcaaag ggcaccagtt cgacggcggc tagctcgtcc
     1621 agcgtcttat gcaccaccgg caggtccagg tggctatcga cggtggtgtc gccgtggccg
     1681 gggaagtgtt tcacgcaggg cgcgatgccc ccctccagga acgccgtcac cgcggccacg
     1741 acatagtcgc cgaccacggc ggggtcgccg ccgaaggccc ggctgccgat gatcgggttc
     1801 agcggattgt tgttcacgtc ggcgacgggg gcgaagtcga gcgtgaagcc gaggcggcgc
     1861 aggcggcgcg ccgtggccgc cgcgcaggcc gccgccacgg gcggcccccc ggctgccgcc
     1921 tgggccatcg ggctgggcgc cgtgagggcg cgcatatcgt tcggcatgcg cgtgacgcgc
     1981 ccgccctcgt cgtccacggc gatgatgaag ggcggcaggt ccagccggcg ggccagggcc
     2041 tgcaactgcc ggttgaggtc gcgcacctgc ccggcgctgc gcaggttgtg gccgaacagg
     2101 atcacgccgc cggggcgcgt ctgctccaca aagcgctcca cgtcgggggt caggctggtg
     2161 tcgttgaagt tgaagaaaaa caactgcccc gcggcagcgc gccggctatc catacgtggc
     2221 tccttctccc gcggggcggg ccagcgcctg ggcggcggcc agggccgggt cggtcacggt
     2281 cacaaccggg gccaccgggc gccgccggcg aatccgccgc accaccgcgc cgcgcagggc
     2341 cggggaggcg accagcaacc cgccgaccag ggccagcggc acggggccgg gaaagtggag
     2401 ctggccggcg gtcgccaggg ccatgatcgt cagttcgttc gccgcctcca c
//
LOCUS       DASUYC010000546         2733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1860159, whole genome shotgun sequence.
ACCESSION   DASUYC010000546 DASUYC010000000
VERSION     DASUYC010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1860159"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..1309
                     /gene="rsgA"
                     /locus_tag="VF276_08960"
     CDS             224..1309
                     /gene="rsgA"
                     /locus_tag="VF276_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEX6607028.1"
                     /translation="MFPESESIPITTPAEAGPPSELVEGTVVDGNRGQYRVETPDGVL
                     LCTLRGKLRKELIHPISHNVLQKARRVNTKARAPVAVGDRVRVLPMGAGKGIIEEILA
                     RTAGAFTREDADVGRVTSVAGIDQMVIVLAVRDPAPHLGLLDRFLVLAEAAEVRAVIC
                     LNKLDLGLDPALAERLDVYRALGYPVILASAETGMGLEALRAELAGHTSALLGPSGVG
                     KSSLLNALEPGLWQRVSTVSSATHKGRHTTSSTRLVPLAGPGGGHLADTAGIRTLALG
                     GAAAGRLDWCFREFRPYLGACYHADCTHRSEPGCAVRAAVDLGALDAERYHSYVRLYD
                     QGGATSGRAWKDVVSSRSLAGPGELRL"
     gene            complement(1473..1889)
                     /locus_tag="VF276_08965"
     CDS             complement(1473..1889)
                     /locus_tag="VF276_08965"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX6607029.1"
                     /translation="MRLLHGIRRGQRMRLGERDLSGPRSRVLAEVAAARPVRMGDLAG
                     RLGLTARTITTLVDALEQEGLIERQADRTDRRATLIDMTPMGCQYYDQVRAGLAELAE
                     QIVGPLSAAERRQLLDLLSRLQLPGGVIDCGPGECC"
     gene            2137..2547
                     /locus_tag="VF276_08970"
     CDS             2137..2547
                     /locus_tag="VF276_08970"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018332567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX6607030.1"
                     /translation="MSVFTEKEIEYLGTQRLGRLATIDSAGNPHVVPVGFRYNPATDT
                     IDIGGHNFSKSRKWAHAQAHPRVAFVVDDVLPPWRPRLIEIKADAELLPSGGESINQA
                     FDSALMRLRPVRIASFGLEPSASGLAVKSRKVER"
BASE COUNT          467 a          958 c          900 g          408 t
ORIGIN      
        1 gtcgcgccca gagcgaaagg aggcgttgct gatgaaaggc atccatctgc tgtatgcagc
       61 gccgccgcgt ctggtccacc ctcggggagg ggcctagcgc ccccgcgccg gcggctccgg
      121 tcgtccttcg ctgcatacag aaccggcggg ttgccccgac cggctttttg tttgtccatc
      181 acaacgtgac tggatgcatc gcgacgcaaa ggagcctgtt gctttgttcc ctgaatccga
      241 atctatccca atcacaaccc ccgccgaggc cgggccgccg tccgagctgg tcgaaggcac
      301 ggtcgtcgac ggcaaccgcg ggcagtaccg ggtcgaaacc ccggacggcg tgctgctctg
      361 caccctgcgc ggcaagctgc gcaaggaact gatccacccc atcagccaca acgtgttgca
      421 gaaggcgcgg cgcgtcaaca ccaaggcccg cgcgccggtg gccgtgggcg accgcgtgcg
      481 cgtgctgccg atgggcgcgg gcaagggcat catcgaggaa atcctggccc gcacggcggg
      541 cgcattcacc cgcgaagacg cggacgttgg gcgggttacg tcggtggccg ggatcgacca
      601 gatggtgatc gtgctcgccg tgcgcgatcc ggctccgcac ctgggcctgc tcgaccgctt
      661 cctggtgctg gccgaggcgg cagaggtgcg cgcggtcatc tgcctcaaca agctcgacct
      721 gggcctcgat cccgcgctgg cggagcggct ggacgtatac cgcgccctcg gctacccggt
      781 gatcctggca tcggcggaga ccggcatggg gctggaggcc ttgcgcgccg agttggccgg
      841 gcacacgtcg gccctgctgg ggccgtcggg cgtgggcaag tcgagcctgc tcaacgcgct
      901 ggagccgggg ctgtggcagc gggtcagcac cgtcagcagc gccacgcaca agggccgcca
      961 caccaccagc agcacgcgcc tggtgccgct ggccgggccg ggcggcggcc acctggccga
     1021 cacggcgggc atccgcacgc tggccctggg cggcgcggcg gccgggcggc tggactggtg
     1081 cttccgcgag ttccgcccct acctgggcgc ctgctaccac gcggactgca cccaccgcag
     1141 cgagccgggc tgcgccgtcc gcgccgccgt cgacctcggc gccctggacg ccgagcgcta
     1201 ccatagctac gtccgcctct acgaccaggg cggcgccacc agtggccgcg cctggaagga
     1261 cgtggtcagc agccgttcgc tggccgggcc gggcgagttg cggttgtagc aacaccacaa
     1321 acacaaaacg accccgccag cgtgtccggc ggggtcgttt ccattccgcg taaagattac
     1381 tgccgccgca ccaaccgccc cagccgcgcc aggtcgctgc ggccgacgct gaggcggcgc
     1441 acggtgattg gcggcctgcc tgcgtgtagc agttagcaac actctcccgg cccgcagtca
     1501 atcacgccgc ccggcagttg caggcgcgac agcaggtcaa gtagctggcg gcgctccgcg
     1561 gcgctgagcg gacccacaat ctgctcggcc agctcggcca ggccggcgcg tacctggtcg
     1621 taatactggc agcccattgg cgtcatgtct atgagcgtgg cgcggcgatc ggtgcggtcg
     1681 gcctgccgct cgatgagtcc ttcctgctcc agcgcgtcca ccagcgtggt gatggtccgg
     1741 gcagtcagcc cgagccggcc cgccagatcg cccatccgca cggggcgcgc cgcggccact
     1801 tcggccagca cccggctgcg cggacccgat agatcccgct cacctaagcg catccgctgc
     1861 ccgcgccgga tgccatgcag caaccgcatc agcctaccga caacctctac tgccgacggt
     1921 gcttcacctg cgggattgtc ttcagattgg gggccagggc tacttgactc cactcttcgc
     1981 ctcgtgtatc atagtgctgc taaaacacat aatccgaaac ctcactatcc gccatcccag
     2041 caaacaggat tggaaccagt ataagcgagg acgagcgagg ttacaagtcc ccaactgcac
     2101 caacaggagg caataaagta ggaggatgaa cagaagatga gcgtattcac tgagaaagag
     2161 atcgagtacc tgggtaccca acgcctgggc cgtctggcaa ccatcgactc ggcgggcaac
     2221 ccgcacgtgg tccccgtcgg cttccgctac aaccccgcga ccgatacgat tgacatcggg
     2281 ggccacaact tctccaagag ccgcaaatgg gcgcacgcgc aagcccaccc gcgcgtggcc
     2341 ttcgtggtgg acgatgtgtt gccgccctgg cggccgcgcc tgatcgagat caaagccgac
     2401 gcggaactgc tgccgagcgg cggagaaagc ataaaccagg ccttcgactc cgcgctgatg
     2461 cgcctgcgcc cggtgcggat agcctctttc ggtctggagc cgagcgcgag cggcctggcc
     2521 gtcaagagcc gcaaggtcga gagataagcc gcactcgaca gcgagcaagg agaaaaccca
     2581 catgtccgtc gaactcactc cgcgcggcac acggggggtt acgttgccga agttgccgcg
     2641 acccctcatg agggtcctct tcccgatcgg gaacgcgctg ttgcgcctgc gcggcgccaa
     2701 gctgctggag ctaacgacca ccggcgcgcg gtc
//
LOCUS       DASUYC010000547         3966 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_571927,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000547 DASUYC010000000
VERSION     DASUYC010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571927"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VF276_08975"
     CDS             complement(<1..216)
                     /locus_tag="VF276_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX6607031.1"
                     /translation="MHAVAQLVSLLINVLMLAILIRAILSWFMQVGRDPFTRLLVDIT
                     EPLLAPIRQLMSRVIPGMFIDFSPFVAI"
     gene            complement(426..1088)
                     /locus_tag="VF276_08980"
     CDS             complement(426..1088)
                     /locus_tag="VF276_08980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEX6607032.1"
                     /translation="MSETIELTVQPRTVTGKDSKKLRRQGVLPGILYGYSIEPQPVQI
                     DGRTFERVYHRAGNVHLVDVRVGETGPATRAFISAVKRDPVTHALIHVDFMAVNLRQE
                     TTANVAIVLTGEAPAARAGDGLLLQNLETLAIKVLPTEVPERIEADVTNLEEVGATIL
                     VKDLPIPAGVTVLTDPEEVVARISAVAVEPVEEVAATEAAEEEAAESGEAAAEAEEEA
                     EG"
     gene            complement(1320..2339)
                     /locus_tag="VF276_08985"
     CDS             complement(1320..2339)
                     /locus_tag="VF276_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX6607033.1"
                     /translation="MALLSRRIGIDLGTANVLVYVNGRGVVLNEPSVVAISTVSGRVK
                     AVGKAAADMIGRTPETIEVVRPMQNGVIADYVVTEAMLKHFIGRAAGRFNLFKPTVMI
                     CIPAGVTSVERRAVKDAAMQAGAQDVFLIREPLAAAIGARVPVSDPNGSLIIDIGGGT
                     TEVAVIALNGIVESNSIRVGGNKLDEYIAQFIKRKYNLLIGDRTAEELKIKIGAALPL
                     EDQMSMDVRGRDQVAGMPRTITVHTNEIVESLAEPLEAIVGAVRTVLERTPPELASDI
                     IDRGMIMTGGGSLLRHIDELMTQITGIPCYVADQPLQCVAVGTGMALEKLDLLRDSLV
                     EEAGA"
     gene            complement(2343..2774)
                     /locus_tag="VF276_08990"
     CDS             complement(2343..2774)
                     /locus_tag="VF276_08990"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEX6607034.1"
                     /translation="MPLHLQIVTAERMVYEDDVDMVIAPAWDGAVGILPHHAPLLTTL
                     QIGELLVRKSGVEQALVIAGGFLEVNEGTVTVLADFAEHAEEVDVQRAEEARRRAQES
                     LAGRSTIENEEAARAELRRAVLQLRVAGTRRRRTQPPQTGA"
     gene            complement(2928..>3966)
                     /gene="atpD"
                     /locus_tag="VF276_08995"
     CDS             complement(2928..>3966)
                     /gene="atpD"
                     /locus_tag="VF276_08995"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX6607035.1"
                     /translation="EQSTSPQVLSTGIKVIDLICPFLKGGKVGAFGGAGVGKTVVIME
                     LIRNIAAEHGGYSVFCGVGERTREGTQLYHEMTDSGVIDKTVMVFGQMNEPPGSRLRV
                     GLTGLTMAEYFRDEGRDLLVFIDNIFRFVQAGSEVSALLGRMPSAVGYQPTLAQEMGQ
                     LQERITSTKQGSITSVQAIYVPADDYTDPAPATTFAHLDGSVTLERSIAEKALFPAVD
                     PLASTSRILDPRIVGEEHYNVAQSVKKVLQRYKDLQDIIAILGIEELSEEDKLIVARA
                     RRIERFLSQPMFVGEVFTGTPGVYVKREDTVRGFREILDGKHDNLPEQAFYMVGTIEM
                     AVEKAAAMAKG"
BASE COUNT          677 a         1287 c         1264 g          738 t
ORIGIN      
        1 gattgccacg aagggggaga agtcgataaa catgccaggg atgacgcgac tcatcaactg
       61 gcgaatgggc gcgagcaagg gttcggtgat gtccaccaac agccgggtga acgggtcgcg
      121 cccgacctgc ataaaccagg agaggatcgc ccggatcaaa atcgccagca tcagcacatt
      181 gatcagcagg ctcaccagtt gggcaacagc gtgcataggt tcactccact tagcgtagta
      241 gccatccagc cctccgtacg cggctggatc actgatatat acggtgggcg ctggggaata
      301 gttgcatcag cctgctatgg gccgatacca ggggcaggac cggacgcccg gcccgcaata
      361 aggcgagggg cacggcgcac cgtgcccctc gtagcgagaa cgcggaagga atggtctcct
      421 gggcgctagc cctcggcctc ttcctcggcc tcggcggcgg cctcgccgct ctcagccgct
      481 tcctcctcgg cggcctcggt cgccgcgact tcctcgaccg gctccacggc caccgcggag
      541 atgcgcgcca cgacctcttc ggggtcggtg agcaccgtca cacccgccgg gatgggcagg
      601 tccttgacca ggatcgtcgc gccgacctct tccaggttgg tgacatcggc ctcgatccgt
      661 tcgggcacct cggtcggcag caccttgatc gccagggtct ccaggttttg caacagcagg
      721 ccatcgcccg cgcgggcggc gggcgcctcg ccggtcagca caatcgccac gttggccgta
      781 gtctcctggc gcaggttgac ggccatgaaa tcgacatgga tcagcgcatg ggtgacgggg
      841 tcgcgcttca ccgcgctgat aaaggcgcgg gttgccggcc cggtctcgcc gacgcgcaca
      901 tcaaccaggt ggacattgcc cgcccggtga tagacccgct caaaggtccg tccatcgatc
      961 tggacaggct ggggctcgat gctatagcca taaagaatgc ccggcagcac gccttgccgg
     1021 cgcagctttt tgctgtcctt gccggtgacg gtgcggggct ggaccgttaa ttcaatcgtt
     1081 tcactcattt tgtgttcacc tctacttctc tagtagttcc caccgtgatt ccgatgggcg
     1141 gcgagccggt ttgaaaccgg cccctacaac gcgcaagtcg catcgtgatt ccgatgggca
     1201 gcaggccggt ttgcaaccgg cccctacaac gcgcaagtcg catcgtgatt ccgataggcg
     1261 gcgagccggt ttcaaaccgg ctcctacaac gcgtatttcg gccacgccgc cgggtaggct
     1321 caggccccgg cttcctcgac cagcgaatcg cgcagcaggt cgagtttctc cagggccatg
     1381 ccggtgccga cggccacgca ttgcaacggc tggtcggcca cataacacgg gatgccggtg
     1441 atctgcgtca tcaactcgtc gatgtggcgc agcaacgagc cgccgccggt cataatcatg
     1501 ccccggtcga tgatatccga ggccagttcc ggtggggttc gttcaagcac ggttcgtacc
     1561 gcgccgacaa tggcttccag gggttcggcc agcgactcca cgatctcgtt ggtatggacc
     1621 gtgatcgtgc gcggcatccc ggccacctgg tcgcgcccgc ggacatccat gctcatctgg
     1681 tcttcgagcg gcagggccgc cccgatcttg atcttgagtt cttcggcggt gcggtcgccg
     1741 atgagcaggt tatacttgcg cttgatgaac tgggcgatat attcgtcgag cttattgccc
     1801 ccgacgcgga tggaattgct ctcgacgatc ccgttcaacg cgatcacggc gacttcggtg
     1861 gtgccgccgc cgatatcgat gatcaagctg ccgttgggat cgcttaccgg cacgcgggcg
     1921 ccgatggcgg cggccagcgg ttcgcggatc aggaacacgt cctgggcgcc ggcctgcatg
     1981 gccgcatctt tgaccgcgcg gcgctccacg ctggtcacgc ccgccgggat gcagatcatg
     2041 acggtgggtt taaacaggtt aaagcgcccc gccgcgcgcc cgatgaaatg cttgagcatc
     2101 gcttcggtca cgacatagtc ggcgatgacg ccgttttgca tggggcgcac gacttcgatc
     2161 gtttcgggcg tgcggccgat catatcggcg gcggccttgc cgaccgcttt cacgcggccg
     2221 ctgacggtgg aaattgcgac gacggaaggc tcgttgagaa cgactcctct tccattgaca
     2281 tagactaaaa cattcgcggt gccgagatcg ataccgatcc ggcgggaaag caatgccatg
     2341 cgttaggctc cggtctgcgg gggttgggta cggcgccggc gggtgcccgc cacgcgcagt
     2401 tgcagcacgg cccggcgcag ttcggcccgt gcggcctctt cattctcgat ggtgctgcgc
     2461 ccggcaaggg actcctgggc gcggcggcgt gcttcttccg cccgctgcac gtcgacttct
     2521 tcggcatgtt cggcgaagtc ggccaggacc gtcaccgtcc cctcgttcac ttcgagaaag
     2581 ccgccggcga tgaccagggc ctgctcgacg ccgcttttac gcaccaacag ctcgccgatc
     2641 tgcagcgtgg tcagcaacgg ggcgtgatgc ggcaagatgc cgacggcgcc gtcccaggcc
     2701 ggggcgatga ccatatccac gtcgtcctcg tacaccatgc gttcggcggt gacaatttgt
     2761 agatgaagag gcatctcgat tctactcctc acgctgcgga actgcgcagc cacatattat
     2821 acacgatccg tccgcgcctg gcgagtcggg cggcaaaacg gaaggccggc aactgaataa
     2881 cgcctgccag tcgccggcct ccccaacatg atccgggttg ttactgctta acccttggcc
     2941 atcgcggcgg ccttctcaac ggccatctcg atggtgccga ccatgtagaa agcctgctcc
     3001 ggcaggttat cgtgcttgcc gtcgaggatt tcgcggaagc cgcgcacggt atcctcgcgc
     3061 ttcacgtaga cgcccggcgt gccggtgaac acctcgccga cgaacatcgg ctggctgagg
     3121 aagcgctcga tgcgccgcgc ccgcgcgacg atcagcttgt cttcttcgct caactcctcg
     3181 atgcccagga tggcgatgat gtcctgcaag tccttgtagc gctgcaacac cttcttcacc
     3241 gactgggcga cgttgtagtg ctcctcgccc acgatgcgcg ggtcgaggat gcggctggtg
     3301 gaggccagcg ggtccacagc cgggaagagc gccttctcgg cgatggaccg ttccaacgtc
     3361 acgctgccgt ccaggtgggc gaacgtggtg gccggggccg ggtcggtgta gtcgtcggcg
     3421 ggcacgtaga tggcctgcac cgacgtgatc gagccctgct tggtcgaggt gatgcgctcc
     3481 tgcaactggc ccatttcctg ggccagcgtg ggctggtagc ccactgcgct gggcatgcgg
     3541 cccagcagcg cggacacttc ggacccggcc tgcacgaagc ggaagatgtt gtcgatgaat
     3601 accagcaggt cgcggccttc gtcgcggaag tactcggcca tggtcaggcc ggtgaggccc
     3661 acgcgcaggc gcgaaccggg cggctcgttc atctggccga acaccatcac cgtcttgtca
     3721 atgacgccgg agtcggtcat ctcatggtac aactgcgtgc cctcgcgggt gcgctcgccc
     3781 acgccgcaga acaccgagta gccgccgtgc tcggcggcga tgttgcggat cagctccatg
     3841 atcacgaccg tcttgcccac gccggcgccg ccgaacgcac cgaccttccc gcccttgagg
     3901 aacgggcaga tcagatcgat gaccttgata ccggtcgaga ggacctgcgg cgaggtggac
     3961 tgctcg
//
LOCUS       DASUYC010000548         2088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1104430, whole genome shotgun sequence.
ACCESSION   DASUYC010000548 DASUYC010000000
VERSION     DASUYC010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104430"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1170
                     /locus_tag="VF276_09000"
     CDS             <1..1170
                     /locus_tag="VF276_09000"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX6607036.1"
                     /translation="REEMVAFARRLIQTPSMSGEEGAVAALVEAEMQRLGFDEVWRDA
                     TGNVIGVMRATAPPAGARPRRIMFNTHMDHVDVGDPARWPYPPYEATVADGAIWGRGA
                     SDLKGPLACQIHAVAALKRLGRPLPNDCVVSAVVQEEVGGLGARELVKEVQTDYCILG
                     EPTTNRIMRGHRGRVEVVVTFSGRSVHASVPAQGANPLYALGRFLQGLELLPMITDPD
                     HPGLGPSSVAPTLLYTDQSSSNVTPAEVVQHLDWRNVPADKPEAILAKVRAVADHSLI
                     DGVHARVEPEPRNLTSYTGVQAAVGAISLPFGIPEDHPLVTTAAQVLSDTFGRPVPVG
                     LWRFATDGGYFVESGTPCIGFSPAWEEVIHTVEERISIDLMVEAFAGYMALGLGL"
     gene            1212..1859
                     /locus_tag="VF276_09005"
     CDS             1212..1859
                     /locus_tag="VF276_09005"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX6607037.1"
                     /translation="MKLDIGTPVRYPDGEQVGTINKVIFDPETNTVHEIVVETPDLVG
                     RLVLVPISMLREDPGDVLTVAADRDAVDALPDYEVEQFAAPPDGWEIPENYVPGDVLF
                     AGTLHYPVVPVIEESNAPAGSVELSQGTEVMCLDGRFGVVDQVLIDDSNQISGLVVRP
                     DDDALPPLLVPPDRITESGSLMVQLNCTIADLAAQSEPYMDPNAEPESDSLMPST"
     gene            1879..>2088
                     /locus_tag="VF276_09010"
     CDS             1879..>2088
                     /locus_tag="VF276_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6607038.1"
                     /translation="MAQQPAQPAAQRPDPPLVPAAPPASPSTPATPLAERPAAPGGTS
                     AASAAPGAAPARRAVIQVQDVTKKFG"
BASE COUNT          335 a          694 c          748 g          311 t
ORIGIN      
        1 cgcgaggaaa tggtggcgtt cgcccgccgg ctgatccaga cgcccagcat gtcgggggag
       61 gaaggcgcgg tggcagccct ggtcgaggcc gagatgcagc gcctgggctt cgacgaggtg
      121 tggcgcgacg ccacgggcaa cgtgatcggc gtgatgcggg ccaccgcgcc gccggccggg
      181 gcccggcccc gccgcatcat gttcaacacg catatggacc acgtagacgt gggcgacccg
      241 gcgcgttggc cctatccgcc ctacgaggct acggtggccg acggcgcgat ctgggggcgc
      301 ggggccagcg acctgaaggg gccgctggcc tgccagatcc atgccgtggc cgcgctcaaa
      361 cgcctgggcc ggcccctgcc caacgattgc gttgtttcgg cggtggtgca ggaggaggtc
      421 ggcgggctgg gggcgcgcga actggtcaag gaggtgcaga ccgactactg catcctgggc
      481 gagccgacca cgaaccgcat catgcgcggc catcgcgggc gggtggaggt ggtggttact
      541 ttcagcgggc gctcggtgca cgccagcgtg cccgcgcagg gcgccaatcc gctctatgcg
      601 ctgggccgtt tcttgcaggg cctggaattg ctgcccatga tcaccgaccc ggaccacccc
      661 ggcctcggcc cgtcgagcgt ggcgccaacc ttgctatata ccgaccagag cagtagcaac
      721 gtgacccccg ccgaagtggt gcaacacctg gattggcgca atgtgcccgc cgacaagccg
      781 gaggcgatcc tggcgaaagt ccgggcggtg gccgatcaca gtcttatcga cggggtccat
      841 gcgcgggtgg agccggagcc gcggaatttg acttcgtaca ccggcgtgca ggccgcggtg
      901 ggcgcgatca gcctgccctt cggcatcccg gaggaccacc cgctggtgac gaccgccgcg
      961 caggtgctga gcgacacctt cgggcggccc gtcccggtgg ggctgtggcg cttcgcgacc
     1021 gacggcgggt actttgtgga aagcggcacg ccctgcatcg gcttttcgcc cgcctgggaa
     1081 gaagtgatcc ataccgtgga ggagcggatt tcgattgact tgatggtcga ggcgtttgcc
     1141 ggctacatgg ccctcgggtt gggcctgtaa cccggcgccc gccgcgcccg ccaatggaga
     1201 ggacgacaag catgaagctg gatattggga cgcctgtgcg ctacccggat ggcgagcagg
     1261 tgggcaccat caacaaggtc atctttgatc cggagacgaa caccgtgcat gaaatcgtgg
     1321 tcgagacgcc ggacctggtc gggcggttgg tgctcgtgcc gatcagcatg ctgcgggaag
     1381 atccgggcga cgtcctgacc gtggcggccg atcgcgacgc cgtggacgcg ctgcccgact
     1441 atgaagtgga gcagttcgct gcgccgccgg acggctggga aataccggag aattacgtgc
     1501 cgggtgatgt actgttcgcg ggcacgctcc attatcccgt ggtcccggtg atcgaggagt
     1561 cgaacgcgcc cgccggctcg gtcgaactga gccagggcac cgaagtgatg tgcctggacg
     1621 ggcgcttcgg cgtggtggac caggtgctga tcgacgactc caatcagatc agcgggctgg
     1681 tggtgcggcc cgacgacgat gcgctgccgc cgctgctcgt cccgcccgac cggatcaccg
     1741 agtccggctc gctgatggtg caactcaact gcacgatcgc cgatctcgcg gcgcagagcg
     1801 agccgtacat ggacccgaac gccgagccgg aatccgactc gctgatgccg tcgacctgac
     1861 gggggcggga ggcccttcat ggcacaacaa ccagcacaac ccgcggccca gcgaccggac
     1921 ccacccctgg ttccggcagc gccaccggcg tcgcccagca cgcccgccac cccgctggcc
     1981 gagcgaccgg ccgcacccgg cggaacctcc gccgcctccg ccgcgcccgg tgcggccccc
     2041 gcgcgccggg cggtgatcca ggtgcaggac gtgaccaaga agttcggc
//
LOCUS       DASUYC010000549         3404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1678067, whole genome shotgun sequence.
ACCESSION   DASUYC010000549 DASUYC010000000
VERSION     DASUYC010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1678067"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1545)
                     /locus_tag="VF276_09015"
     CDS             complement(<1..1545)
                     /locus_tag="VF276_09015"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEX6607039.1"
                     /translation="MSTRQSTPQSGHHAATTDSPLGCSRVAVFGLAAVAGAAERLAEG
                     GVRHWQLSDAASTGPAHALAHLWGWEAVGVPASAVFARVLPRRVADPAALSFGALPPL
                     DDAHYDDLRVALAAERPDLLLGGGDSATLLLLGRLARDLDLPAVLVALPAAASPRAMV
                     LALLPGDGGNGRDVADLPAYRNGAPLGDPANDLNTPERRLDWQDANLHAAALARLLLL
                     GEAAAPADLGRIVFGERRTLLVQGAAGWPWRVQYLDLARAAALSPPAPAPAPLGRQSA
                     RGPILVVGCGRIGSQAARSLLAAGATPALALIEGGRVDAGDLSGGFYTADQVGKNRAD
                     ALAQQLIRIALKPHTTPEWTPAAFTDDWSGLLRASGDWCFTQVPRDAKPGPWFESLLD
                     TLRPVAVILALGPYEDGRLARVLRDKGVPHVVSRCAADGAWFEALVVDGSRGPCRECM
                     QAGALPIVGAPTATSIETGRAANLVARLAWQLSLDSAARAPWFARLLAEEQTALMGWN
                     GTAGSAA"
     gene            complement(1766..2362)
                     /locus_tag="VF276_09020"
     CDS             complement(1766..2362)
                     /locus_tag="VF276_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012900609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HEX6607040.1"
                     /translation="MPAEYTHWLLFGAICGATWVLALYLWPRLLLFVYKRAILVKGFD
                     DGPIPVNTLYTEPQNLFADPLGAQTAASSNLMTVGVNHDTLAAVGWLDLAQGPQVLHV
                     PDMATRYYSVQFTDPAKNTNFAYVGTRTTGTAAGDYLVTGPGWKGVVPPGMIQIAAPN
                     KSVLVLGRVLVASDADLPTAYGLAKQIQLTPLNQEHPI"
     gene            complement(2362..3024)
                     /locus_tag="VF276_09025"
     CDS             complement(2362..3024)
                     /locus_tag="VF276_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HEX6607041.1"
                     /translation="MNRTSTYLAILVGVVCGWFLLNFFLGTHVRTVRNDVVQGILIGF
                     GLAFVTAQLFARIKATRVNGWITMFGLGEPGNGMLLRAAHAQLFPGPVNVPQEAMYWW
                     TIVDGAGHTLSGAHAYRLRFPPGGLPPHRAFWSLTMGTANNHFVPNALKRYSVSDRSG
                     LVPNPDGSVDIYIQHTAPAGHESNWLPAPAGKFVLWLRVYIPGEAILQGAYTVPPVVA
                     VS"
     gene            complement(3129..>3404)
                     /locus_tag="VF276_09030"
     CDS             complement(3129..>3404)
                     /locus_tag="VF276_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6607042.1"
                     /translation="AIVGFGLIGAGVACPFPLVLSAAARTPGIAAGRAIAAMATVAYM
                     GSFIGPPLIGSVAEILTLRGALSILVLVGLLMLLIGGRVQPQQQDGA"
BASE COUNT          574 a         1179 c         1153 g          498 t
ORIGIN      
        1 cgccgcactg cccgccgtcc cattccagcc catgagcgcc gtctgctcct cggccagcag
       61 gcgcgcgaac cagggcgcgc gggccgccga gtccaggctc agttgccagg ccagccgcgc
      121 caccaggttg gccgcccgcc ccgtctcgat gctggtggcg gtcggcgcgc ccacaatcgg
      181 cagggcgccc gcctgcatac attcccggca cggcccccgc gatccatcga ccaccagtgc
      241 ctcgaaccag gcgccgtccg ccgcgcagcg actcacgaca tggggcaccc ccttgtcgcg
      301 cagcacccgc gccagccggc cgtcttcgta gggaccgagg gccaggatca ccgcgaccgg
      361 gcgcaacgtg tcgagcaacg actcgaacca cgggccgggc ttcgcatcgc gcggcacctg
      421 cgtgaagcac cagtctccgc tcgctcgcag caggccgctc caatcgtcgg tgaaagcggc
      481 gggcgtccac tccggcgtcg tgtgcggctt gagcgcgatg cggatcagtt gctgggccag
      541 ggcatccgcg cggttcttgc cgacctggtc ggcggtgtag aagccgccgc tgaggtcgcc
      601 cgcatccacc cgcccgccct cgatcagggc cagggccggg gtggcccccg ccgccagcag
      661 gctgcgcgcc gcctggctgc cgatgcgccc gcaaccgacc accaggatcg ggccgcgcgc
      721 cgattggcgg cccagggggg cgggagcggg ggccggcggg ctcagagccg ccgcgcgagc
      781 caggtcgaga tactggaccc gccagggcca tcccgccgcg ccctgcacca gcagcgtccg
      841 ccgttcgccg aacacgatgc gccccagatc ggcgggcgcg gcggcctcgc ccagcaacag
      901 caagcgggcc agggccgccg catgcaggtt ggcgtcctgc caatccaggc ggcgctccgg
      961 cgtgttgagg tcgttcgccg ggtcgccgag cggcgcgccg ttgcgatagg ccggtaggtc
     1021 ggcaacatcg cgcccattgc cgccgtcgcc cggcagcagc gccagcacca tcgcccgcgg
     1081 actcgcggcg gccggcaacg ccaccagcac ggcgggcagg tccaggtcgc gggccaggcg
     1141 accgagcaga agcagcgtcg cgctgtcgcc gccccccagc agcaggtcgg gccgctccgc
     1201 cgcaagcgcc acgcggaggt cgtcgtagtg ggcgtcgtcc agcggtggca gcgcgccaaa
     1261 gctcagcgcg gcgggatcgg ccacccgccg gggcaacacg cgggcaaata cggccgacgc
     1321 gggcacgccc acggcctccc agccccacag gtgggccagc gcgtgcgccg ggccggtgga
     1381 cgccgcgtcg ctaagctgcc agtggcgcac gcccccttcg gccagccgct cggcggcgcc
     1441 cgccaccgcc gccaacccga acaccgccac ccggctgcac cccaggggcg agtcggtcgt
     1501 tgctgcatgg tggccggatt gcggggtact ttgccgcgta ctcatggcgc cgatcctttc
     1561 gctatcgtgt agtagctaca ctaactacag cccctaaaaa agggagccgt tctcccctgc
     1621 cggcggacag ggaacaagcc ccaggttgcc tcttagtgtc acttacacat ttactatagc
     1681 acagttctac gcggctgtca atacttttgc ctcaccaatc ggaaccagag accgcgccgg
     1741 gccgcatcgc aaatctatgg taagatcaga tgggatgttc ctggttgagt ggggtcagtt
     1801 ggatctgctt tgccaggcca taggcggtcg ggagatcggc gtcgcttgcc accaggacac
     1861 ggccgaggac cagcaccgac ttgttcgggg cggctatctg gatcatgccc ggcggcacca
     1921 cccctttcca gccggggccg gtcacgaggt agtcgcccgc tgcggttccc gtggtgcggg
     1981 tgccgacgta ggcaaaatta gtgtttttcg ccggatctgt gaattgcacg ctgtagtagc
     2041 gcgttgccat gtccggcaca tgcagaactt gcggcccctg ggcgagatcg agccagccga
     2101 ccgcggccag cgtgtcgtgg ttcacgccca ccgtcatcaa attcgagctt gcggccgttt
     2161 gggctccgag ggggtcggca aagaggtttt gcggctccgt atagagcgta ttgaccggga
     2221 tggggccgtc atcgaacccc ttcacgagga tcgcccgctt gtacacgaac agcagcaggc
     2281 ggggccacaa gtagagggcc agcacccagg tcgcgccgca gatcgcgccg aatagcagcc
     2341 aatgcgtgta ctccgccggc attagctcac cgcgaccacc ggaggcaccg tatacgcgcc
     2401 ctggagaatg gcctcgccgg ggatatatac gcgcagccag aggacgaatt tgccggcggg
     2461 cgcgggcagc cagttcgact cgtgcccggc cggggcggtg tgctgaatgt aaatgtcgac
     2521 cgaaccgtcg gggttgggca cgaggccgga ccgatcgctg acgctgtagc gcttgagcgc
     2581 attcggcaca aaatgattgt tcgccgtgcc catcgtcagc gaccagaatg cccggtgcgg
     2641 tggcagcccg cccggcggaa agcgcaggcg ataggcgtgc gcgccggaga gggtatggcc
     2701 cgcgccatcg acaatcgtcc accaatacat cgcctcttgc gggacattca ccgggccggg
     2761 aaagagttgg gcgtgcgcgg cgcgcaacaa catgccgttg cccggctcgc caaggccgaa
     2821 catagtgatc cagccgttga cccgcgtggc cttgatccgt gcgaacagtt gggccgtgac
     2881 aaaggccagg ccgaagccga tgaggatgcc ctgcaccacg tcgttacgca ccgttcggac
     2941 gtgtgtaccg aggaagaagt tgaggaggaa ccagccgcat accacgccga cgaggatcgc
     3001 cagataggtc gaggttctgt tcatattatg attttatcac gcccggttta gcggcgcaat
     3061 gggcgggagg tcaggcccag gcgagtttct cgcccgcctg ttgacgatcg gctttggtac
     3121 tcttgcggtt acgctccatc ttgctgctgg ggctgcaccc ggcccccgat cagcagcatg
     3181 aggaggccga caaggacaag gatgctcagg gcacctcgca gggtgagaat ctcggcgaca
     3241 gagccgatta gcggcgggcc gatgaacgac cccatgtagg ccaccgtcgc catggccgcg
     3301 atggcgcgcc ccgcggcgat gccgggggtg cgggcggccg cgctgaggac taagggaaaa
     3361 gggcacgcca ctcccgcgcc gatcaaccca aaaccgacga tagc
//
LOCUS       DASUYC010000550         2414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_57133,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000550 DASUYC010000000
VERSION     DASUYC010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57133"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="VF276_09035"
     CDS             complement(<1..871)
                     /locus_tag="VF276_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607043.1"
                     /translation="MRPASIVLRLVPPLLLLLGLTAILGAPAKAHPPGPSSGSPLAGN
                     LVVNGDFETGHLSPWTVPYPASDPGAGVIVTSPVHTGTHALQVITNAQAGVASGVAGG
                     GNCDPVGLRIPVQPNATYSWWAWIFVPQGTQLDSAYMRVAWYPAANCDGGVQIDSQRS
                     HPVTTASGTWIQSSGTAVAPPNAGYAEIRLGAVPVSTTSITVYFDDVTLVQIPDTPTP
                     TNTPLPTPTPHAGHFEDVPPANPFYSYIECIGSRDLISGYTCGTTPDEPCIGPENKPY
                     FRPTNNLTRGQAAK"
     gene            complement(1164..2309)
                     /locus_tag="VF276_09040"
     CDS             complement(1164..2309)
                     /locus_tag="VF276_09040"
                     /inference="COORDINATES: protein motif:HMM:NF027641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0051118 - glucan
                     endo-1,3-alpha-glucosidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,3-alpha-glucanase family
                     glycosylhydrolase"
                     /protein_id="HEX6607044.1"
                     /translation="MMLLKRSFTLRWWGLSIWLLGAALLPLPSAAQPQPPASPTVSRA
                     PIPLLAYYYIWFDPSSWDRAKIDLPELGPYSSDDPKVMRQHIDWAKEAGIDGFIVSWK
                     STPTLNRRLQQLMEIADAADFKLVMIYEGLDFNRNPLPVAQVDADFDYFLDHFAGDKA
                     FRLFSKPLAIWSGTWKFSPDEIAKVTGPRRDRLTILATEKSVADYERIAALVDGDAYY
                     WSSVNPDTYPNYGDKLNDMANAIHARSGLWIAPAAPGFDARLVGGTRAVDRKDGATLR
                     LQLATAFAASPDAVGLISWNEFSENSYVEPSQKFGRRYLDVLRDIRTTVAPNVENLDS
                     SAPGDIQPRIDTLLPLVGLAALLIGSIAVLVWRRWRRKNTGSMSMRF"
BASE COUNT          393 a          732 c          864 g          425 t
ORIGIN      
        1 ttttggccgc ctggccccgc gtcaggttgt tcgtggggcg gaagtagggc ttgttctcgg
       61 gcccgataca cggttcatcg ggcgtggtgc cgcaggtata cccgctgatg agatcccggc
      121 tgccgatgca ctcgatgtac gaatagaacg ggttggccgg cggcacatct tcgaaatgcc
      181 cggcgtgggg cgtcggcgtg ggcagcggcg tattggtcgg ggtgggcgtg tccggtatct
      241 gcaccagcgt cacgtcgtca aagtagactg tgatggacgt ggtggacacc gggacggcgc
      301 ccaggcggat ctcggcgtac ccggcattgg gcggcgcgac ggccgtgccc gagctttgta
      361 tccaggtgcc cgacgcggtg gtcaccgggt ggctgcgctg ggagtcgatt tgcacgccgc
      421 cgtcgcagtt ggccgcgggg taccaggcca cgcgcatata ggcgctgtcg agttgtgtgc
      481 cctggggcac aaagatccag gcccaccagc tataggtggc attgggctgc acggggatgc
      541 gcaggcccac cgggtcgcaa ttgcccccgc cggccacgcc gctggcaacc ccggcctgcg
      601 cgttggtgat cacctgcagg gcgtgagtgc cggtatgcac cgggctggtg acgatgaccc
      661 ccgcgcccgg atcgctcgcc ggatagggca cggtccaggg actcagatgg ccggtctcaa
      721 aatccccgtt caccaccagg ttgccggcaa ggggtgaccc gctgctgggc cccggcggat
      781 gggccttggc cggggcgccc aggatagccg tcagtcccag tagcagcagc aatgggggca
      841 cgagccgcaa cacaatcgat gcagggcgca tggtgatttc ctcctggtaa cgatgagcga
      901 aatccgaggt ctcgcgtgac gggcgatccg gacacctggg aacccttaga agcgcatccc
      961 cccggcttgt cgttctgagt ggagcgcggt ctctcgatgg ggagacgaga ctctgcgctg
     1021 cgcccagagt gacaagcctg tagctcgtct tcgacggtca tggcgctatt gtagcaggtt
     1081 tgccagcctg ccgttgcgag tctgaaccgt tacggggccg gcccgcgcgg cgctcagagt
     1141 gacaggccac ggtgccggcc tgctcagaag cgcatggaca ttgatcccgt attcttccgg
     1201 cgccagcggc gccataccag gaccgcgatg ctgccgatca gcaacgcggc cagcccgacc
     1261 agcggcagca gggtgtcgat gcgcggctgg atgtcgcccg gcgcgctgga gtcgaggttc
     1321 tcgacgttgg gcgccaccgt cgtgcggata tcgcgcaaga catccaggta gcgccggcca
     1381 aatttctggc ttggttcgac gtaggagttc tcgctgaatt cgttccaact gatcagcccg
     1441 acggcatcgg gcgaggccgc gaacgccgtc gccagttgca gccgcagggt cgcgccgtcc
     1501 ttgcggtcca ccgcgcgggt gccgcccacg agccgggcgt cgaagccggg cgcggcgggg
     1561 gcgatccaga ggccgctgcg cgcgtggatc gcgttcgcca tgtcgttgag tttgtccccg
     1621 tagttggggt aggtgtccgg gttcacggag gaccagtagt aggcgtcgcc gtcgacgagg
     1681 gcggcgatgc gctcgtagtc ggccacgctt ttctcggtgg cgaggattgt tagccggtcg
     1741 cgccgggggc ccgtcacctt cgcgatctca tccggcgaaa acttccaggt gcccgaccag
     1801 atcgccagcg gcttgctgaa gaggcggaac gccttgtcgc cggcgaagtg gtccaggaag
     1861 tagtcgaaat cggcatcgac ttgggcgacg ggcagcgggt tgcggttgaa gtccagcccc
     1921 tcgtagatca tcaccagctt gaaatcggcg gcgtcggcga tctccatcag ttgttgcagg
     1981 cgccggttga gcgtaggcgt gctcttccaa ctgacgatga agccgtcaat acccgcttcc
     2041 ttggcccaat caatgtgctg gcgcatgacc ttggggtcgt cgctggagta cggccccagt
     2101 tccggcagat cgatcttggc gcggtcccag gagctggggt cgaaccagat gtagtagtag
     2161 gccagcaggg ggatgggggc gcggctcaca gtgggcgagg ccggcggttg gggctgggcc
     2221 gcggacggca gcggcagcaa ggcggcgccc agcaaccaga tcgacaggcc ccaccagcgt
     2281 aacgtgaacg agcgtttcaa gagcatcatg gtttcctcag ataggaatat ggacgtaggg
     2341 gcggaccggc gtgtccgccc tgccgcggcg cgggcaaacg ataaaggatc ctcctatcgc
     2401 taagggatcc agcc
//
LOCUS       DASUYC010000551         2028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1647918, whole genome shotgun sequence.
ACCESSION   DASUYC010000551 DASUYC010000000
VERSION     DASUYC010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1647918"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..>2028)
                     /locus_tag="VF276_09045"
     CDS             complement(201..>2028)
                     /locus_tag="VF276_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HEX6607045.1"
                     /translation="ALVGFGSAKGSNEEAYLFQKLVRTGFRSNNVDHCTRLCHASSVA
                     ALLEGIGSGAVSNPVMDVTQAEVIIVIGSNPTVNHPVAATWMKNAAKSGAKLIIMDPR
                     KSDLTRLAYRHLQFKPSTDVAMLNAIMHVIVHEGLVDKDFIASRTIGYDELKQNLVGY
                     SPDEMAPVCGIDAETLREVARLFATSKASMILWGMGVSQHVHGTDNARCLIALSLMTG
                     QVGRPGTGLHPLRGQNNVQGASDAGLIPMMFPDYQKVSEPAVRASFEKHWGLQPGTLD
                     PQPGLTVVEVMHAIGEGKVRGMYVMGENPAMSDPDANHAREMLAKLDHLVVQDIFLTE
                     TACLADVVLPATAFPEKTGTFTNTDRMVQLGRQAIDPPGEAKQDLWIIQEMARGLGLD
                     WDYKHVSEVFDEMRHSMPSIGGITWERLEREDSVTYPCTNEGDPGEPVVFVDDFPREG
                     GKARFVPADIVPAAERPDPQYPMVLITGRQLEHWHTGSMTRRATVLDAIEPDPVALVH
                     PLDLAAIGVKPGEVITLESRRGKVSLYARADETSPRGAVFVAFCYYEAAINRLTNDKL
                     DPVGKIPELKYCAIRVTAGGTVPEQVSFGGGQLLRKLEENVV"
BASE COUNT          298 a          663 c          716 g          351 t
ORIGIN      
        1 cggccctccc ggaacggcgg gattgtagaa atgggttggg gaagggcgag aggcgagccg
       61 accgggggag caggggattg cgcgagcggt ggggcgggcg ggcaaaaaga aaagcgccgc
      121 agtgcggcgc tttctatggg agccctgggt tcccgccttc gcggggatga cgaggggagg
      181 gcggggatga cgggagggga tcagaccacg ttctcttcca gcttccgcag caactgcccc
      241 ccgccaaagc tcacctgctc cggcaccgtg ccgcccgcgg tgacgcggat ggcgcaatac
      301 ttcagctcgg ggatcttgcc caccggatcc agcttgtcgt tggtcagccg gttgatcgcg
      361 gcttcgtagt agcagaaggc gacgaacacg gctccgcgcg gcgaggtctc gtcggcgcgg
      421 gcatacagcg ataccttgcc gcgccgcgac tcgagggtaa tgacttcgcc cggcttcacg
      481 ccgatggcgg ccaggtccag cgggtgcacc agggccaccg gatcgggctc gatcgcatcg
      541 agcacggtgg cgcggcgcgt catgctgccc gtgtgccagt gttcgagctg gcggccggtg
      601 atcagcacca tcgggtactg cgggtccggg cgctcggcag ctggcacgat gtcggccggc
      661 acgaagcgcg ccttgccgcc ttcgcgcggg aagtcgtcga cgaagacgac cggttcgccc
      721 gggtcgccct cgttcgtgca ggggtaggtg acggagtctt cgcgctccag ccgctcccac
      781 gtgatgccgc cgatgctggg catgctgtgc cgcatctcgt cgaacacttc cgacacgtgc
      841 ttgtagtccc agtccaggcc caggccgcgt gccatctcct ggatgatcca caggtcctgc
      901 ttcgcctcgc cgggcgggtc gatcgcctgg cggcccagct gcaccatccg gtcggtgttg
      961 gtgaaggtgc cggtcttctc ggggaaggcg gtcgcgggca gcaccacgtc ggccaggcag
     1021 gccgtttcgg tcaggaagat gtcctgcacg accaggtggt ccagcttcgc gagcatctcg
     1081 cgcgcatggt tggcgtccgg gtcggacatg gcggggttct cgcccatcac gtacatgccg
     1141 cgcaccttgc cttcgccgat ggcgtgcatc acctccacca ccgtcaggcc cggctgcggg
     1201 tcgagcgtgc cgggctgcag gccccagtgc ttctcgaagc tcgcgcgcac cgccggttcc
     1261 gagaccttct ggtagtccgg gaacatcatg ggaatcaggc cagcgtcgga cgcgccctgc
     1321 acgttgttct gcccgcgcag ggggtgcagg ccggtgccgg ggcggccgac ctggcccgtc
     1381 atcagcgaca gggcgatcag gcagcgggcg ttgtcggtgc cgtgcacgtg ctgcgacacg
     1441 cccatgcccc acaggatcat ggaagccttg gacgtcgcga acaggcgcgc cacttcgcgc
     1501 agcgtctcgg catcgatgcc gcacaccggc gccatctcgt cggggctgta gcccaccagg
     1561 ttctgcttca gttcgtcgta tccgatggtg cgcgaggcga tgaagtcctt gtcgaccagg
     1621 ccctcgtgca cgatgacgtg catgatggcg ttgagcatcg ccacgtcggt cgacggcttg
     1681 aactgcaggt gccggtaggc caggcgcgtc aggtccgact tgcgcgggtc catgatgatc
     1741 agcttggcgc cgctcttggc cgcgttcttc atccaggtgg cggccaccgg gtggttcacc
     1801 gtggggttgg agccgatcac gatgatcacc tcggcctgcg tcacgtccat cacggggttg
     1861 gacacggcgc cggagccgat gccttccagc agcgccgcca ccgacgatgc gtggcacagg
     1921 cgcgtgcagt ggtcgacgtt gttgctgcga aagcccgtgc gcaccagctt ctggaacagg
     1981 tacgcctctt cgttgctgcc cttggcggag ccgaagccca ccaacgcc
//
LOCUS       DASUYC010000552         2338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1673250, whole genome shotgun sequence.
ACCESSION   DASUYC010000552 DASUYC010000000
VERSION     DASUYC010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1673250"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..616)
                     /locus_tag="VF276_09050"
     CDS             complement(41..616)
                     /locus_tag="VF276_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607046.1"
                     /translation="MPTQLIAPETGAPPPVEPAPPAVPPAPAPATPAPPVTTGGPPAW
                     MQPLIKAANPLIVGLLRSPLHPLLSSRILVLSFRGRNTGRRFRIPVSYDRQGATVSVY
                     TRHAWWKNLRGGVPVEVVLRGHTLAARAEAVTDPAEVTAAVTAFIAAHGPAQAWQIAV
                     ALAPDRVPTPASLAAAAQGLVLVRVHLPGVP"
     gene            complement(827..>2338)
                     /locus_tag="VF276_09055"
     CDS             complement(827..>2338)
                     /locus_tag="VF276_09055"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6607047.1"
                     /translation="PAPTATAVPPAATAAPPTPTPPAPTAPVARVPRFEPAPCPFDVP
                     GDRLEGRDVRCGYLVVPEQHSVPAGPTIKLAVAIFKARGDTVQPDPIVYLQGGPGGSS
                     QGPILGFQGAYAKALGQDRDVIYFDQRGTGYSQPNLACPEVAQQGIADYAANLTVAEE
                     SAHQMAALEHCRYRLASQGVNLAAYHTTENAADLNDLRVALGLRQLNLLGGSYGSRLG
                     LITMRDYPDAVRSAVLQGIYPPPQNHDEQMPRSFDRALTLVFDACAADPACAAAYPDL
                     PGTFSAVVRDLAAHPVTLQVAAGERTYPLVASGHRFTQVLRALVYQVAGIQQIPSLIY
                     RVHRGDTAPLAPLAAQAIALSNSSALGMYYSTECNEETPFNSRAGLAAALRDVRPEIR
                     DAFNWYQDLTVCESWPSGTAPARENAPVVSAVPALLLTGWYDPVTPPAYGESAVATLG
                     QGHHVLFPADAHGVEGNAPACAVSLFAAFIHDPQAPLDAGCAQALRLHFVLPP"
BASE COUNT          316 a          784 c          928 g          310 t
ORIGIN      
        1 ggtggggtat accccagccg gggggagagg ttgcgccgcg ctacggcacg cccggcaaat
       61 ggacccgcac caggacgagg ccctgggcgg cggcggccag gctggccggg gtgggcacgc
      121 gatccggcgc cagggccacg gcgatctgcc aggcctgcgc cgggccgtgc gccgcgataa
      181 aggcggtcac ggcggcggtc acctccgccg ggtcggtcac ggcctcggcg cgggcggcca
      241 gcgtgtggcc gcgcagcacc acctcgaccg gcaccccgcc gcgcaggttc ttccaccagg
      301 cgtggcgcgt gtagaccgac acggtggcgc cctggcgatc atagctcacc gggatgcgga
      361 accggcgccc ggtgttgcgc ccgcggaagc tgagcaccag gatccgggaa ctcaacagcg
      421 ggtgcagcgg cgagcgcagc agcccgacga tgagcgggtt ggccgccttg atcagcggct
      481 gcatccaggc gggcggcccg ccggtggtca ccgggggggc cggcgtcgca ggcgccggag
      541 ccggggggac tgccggcggc gcgggctcca ccgggggcgg tgcgcccgtt tcgggcgcga
      601 taagttgcgt tggcatcgtg gtacctccag atttcagtag tcagtggtca gtaatcagtg
      661 gtcaggggtc agctagcatg cttcgtttag ctatcagccg tgtgctacgc accgcggcgc
      721 gaacagaaga cgagcggtca gccggcacgc cccacactgc ccactgccca ctgcccactg
      781 accactgccc actgaccact gaccactcgt cgtcactcag cactcgtcac ggcggcaaga
      841 cgaagtgcag gcgcagggcc tgggcgcaac cggcgtcgag cggggcctgc gggtcgtgga
      901 tgaaggcggc gaagagcgac acggcgcagg ccggcgcgtt gccctccacc ccatgggcgt
      961 cggcggggaa cagcacgtgg tggccctggc ccagcgtcgc cacggcggat tcgccgtagg
     1021 ccggcggcgt caccgggtcg taccacccgg tcagcagcag ggcgggcacg gcgctgacca
     1081 cgggcgcgtt ctcgcgggcc ggcgcggtgc cgctgggcca cgattcgcag accgtcaggt
     1141 cctggtacca gttgaaggcg tcgcggatct cgggccgcac gtcgcgcaga gcggcggcca
     1201 ggccggcccg gctgttgaac ggcgtctcct cgttgcactc cgtcgagtag tacatgccca
     1261 gggccgaact gttcgacagc gcgatcgcct gcgcggccag cggcgccagc ggcgcggtgt
     1321 cgccgcgatg cacgcggtag atcaggctgg ggatttgctg gatcccggcg acctggtaga
     1381 ccagcgcgcg caagacctgg gtgaagcggt ggccgctggc caccagcggg taggtacgct
     1441 cgcccgcggc cacctgcagc gtcaccgggt gggctgccag gtcgcggacc acggcgctga
     1501 aggtgccggg caggtcggga taggcagcgg cgcaggcggg gtcggcggcg caggcgtcga
     1561 agaccagcgt gagcgcgcgg tcgaagctgc gcggcatctg ctcgtcgtgg ttctgcggcg
     1621 gcgggtagat gccctgcagc acggcgctgc gcacggcgtc cgggtagtcg cgcatggtga
     1681 tcaggcccag gcgcgagccg taggacccac ccagcaggtt gagctggcgc aggcccaggg
     1741 ccacgcgcag gtcgttgagg tcggcggcgt tctcggtggt gtggtaggcg gccaggttca
     1801 cgccctggct cgccagccgg tagcggcagt gttccagcgc ggccatctgg tgcgccgact
     1861 cctcggccac ggtcaggttc gcggcgtagt cggcgatgcc ttgctgcgcc acctccgggc
     1921 aggccaggtt gggctgcgaa tagccggtgc cgcgttggtc gaagtagatc acgtcgcggt
     1981 cctggcccag ggctttggcg taggcgccct ggaagcccag gatcgggccc tggctgctgc
     2041 cgcccggccc gccctgcagg tagacgatgg ggtcgggctg gaccgtgtcg ccgcgcgcct
     2101 tgaagatcgc cacggccagc ttaatggtgg gcccggcggg cacgctgtgc tgctccggca
     2161 cgaccaggta gccgcagcgg acatcgcgcc cctccagccg gtcgcccggc acgtcgaagg
     2221 ggcacggggc cggctcgaag cgcggcacgc gggccacagg cgccgtgggc gcgggcgggg
     2281 tgggggtggg cggcgcggcg gtggccgcgg gcggcacggc ggtggccgtg ggcgcggg
//
LOCUS       DASUYC010000553         3923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_724973,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000553 DASUYC010000000
VERSION     DASUYC010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724973"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1550)
                     /locus_tag="VF276_09060"
     CDS             complement(<1..1550)
                     /locus_tag="VF276_09060"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEX6607048.1"
                     /translation="MSTQRWIEDVFRPVIVGLMVGSIVLAIVQMLHLVIPTWDGTYLA
                     VFCGLVAAESYFSYRFAPKGSWDTVTKLRYRGSEALVIMLLLQLSTNISEGRANIFED
                     LPRLDAPMLIAMTIVALCWFFAADTAADLERVGEPPEQTPAYVPPLDTLTSRFLVGGA
                     ILLVATGLSKIRLERLFDLGRAPIGGLVLHVLVYFLLGVILLGQVRYTELSRRWEARG
                     TRMAPELPAGWVRYSVIFVALVALLAFVLPTSYTVGLLDVLRTILGWIVTIVTTIALL
                     IMTGLSLLASAILSLFGVKTQPMQFQPAPPPPPIPPPPTGDDGGAWLEFAKSALFWVA
                     VIAVLVYLARSYWQSGPRLRQILAAFAPLQRVFRWWAGVWRGVRRSLAVVGEQIGRLL
                     PARSTAAGGSPLGRLLRVRGLSPREQVLYYYLSIVRRAGRQGYPRHGTQSPREFSAGL
                     APQLPQAQEDLDRLTQEFIEARYSRHPIPPTEANQAQTHWQQVKAALQRARDQVTEAK
                     PPPGAGPSG"
     gene            complement(1547..2734)
                     /locus_tag="VF276_09065"
     CDS             complement(1547..2734)
                     /locus_tag="VF276_09065"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX6607049.1"
                     /translation="MRNFVPLLVVLLVIALALRVDFFVTIVYFLAALYVLSRIWMGRA
                     LRHLGVRRAFTDHAFTGDLVAVDLTVRNGGWLPIPWVEVSESLPVRLMAVPFDRQVIT
                     LGPYGEWHHRYTLACRHRGYYDLGPLSLELGDLLGLARRTGQGAEPKRLTVYPRVVAL
                     HELGLPTHSPMVALPVQSPLFEDASRIMGVRDYQRGDALRRLHWTATARTGRLVVKQY
                     QPAIARETLICLDLDEDDYEITRRYPGTELAIVAAASIASHIILKEGLAVGLITEAVD
                     PLVGAERYIALPPRAERAHLMTNILETLARVQMTQGAAFAELLHRAGVHASWGTTFVV
                     ITGGASQALFDNLLGLRRRGFSVTLILAQPDVAAHEWQEQAARIGMPLLEAWSDRDLE
                     TWR"
     gene            complement(2747..3712)
                     /locus_tag="VF276_09070"
     CDS             complement(2747..3712)
                     /locus_tag="VF276_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX6607050.1"
                     /translation="MATTTTTTPLTQFAERVIQNVETVIVGKRAQIEYVLVALLCQGH
                     VLIDDVPGTGKTMLARAVAASMGLAFKRLQCTPDLLPNDVTGVSVYNQQANTFEFQPG
                     PVFVNFLLVDEINRATPRTQSALLEAMQEQQVTVDGVTRRLPAPFVVLATQNPIEFEG
                     TFPLPEAQLDRFLIHMTMGYPGSGDELQILRNLRKQHPIEQIGQVVDGGELPALADLV
                     TDVHVDETLEQYILGIVRATRSHPDIAVGASPRGTLALYKTAQAFAALHGRDYVVPED
                     VREMAPLTLPHRISIKPESQLRGRDARTIVADIVQHTTLDLGDKQ"
BASE COUNT          654 a         1329 c         1343 g          597 t
ORIGIN      
        1 ccggatgggc cggcgccggg gggcggtttc gcctcggtca cctggtcccg cgcgcgctgc
       61 aaggccgcct ttacctgctg ccaatgggtt tgcgcctggt tcgcctcggt cggcgggatc
      121 gggtggcggc tgtaacgcgc ttcgatgaac tcctgcgtga gccggtccag gtcttcctgc
      181 gcctggggta gttgtggggc cagccccgcg ctgaattcgc ggggcgactg ggtgccgtgg
      241 cggggatagc cctgccgccc cgcccggcgc acgatgctca ggtagtagta cagcacctgt
      301 tcgcgcggcg acagcccgcg cacgcgcagc aggcgaccca gcggactgcc cccggcggcg
      361 gtgctgcgcg ccggcagcag gcgcccgatc tgctcgccta ccaccgccag cgaccgccgg
      421 accccgcgcc agacgcccgc ccaccaccgg aacacccgtt gcaggggcgc gaaagccgcc
      481 aggatctgcc gtagccgcgg gccgctctgc caatagctgc gcgccaggta gaccagcacg
      541 gcgatcacgg ccacccagaa cagggccgat ttcgcgaact ccagccacgc gccgccgtcg
      601 tcgccggtgg gcggcggggg tatgggtggc gggggcggcg cgggctggaa ctgcatgggt
      661 tgcgttttga cgccgaacaa agagaggatc gcgctcgcca gcagcgacag gccggtcatg
      721 atcagcaggg caatggtggt gacgatggtc acgatccagc ccagaatcgt ccgcaacacg
      781 tccagcaggc cgacggtgta gctggtgggc agcacgaacg ccagcagcgc gaccagcgcg
      841 acgaagatca cgctgtagcg cacccagccc gccggtagct ccggcgccat gcgcgtgccc
      901 cgcgcctccc agcgccgcga cagttccgtg tagcgcacct gccccagcag gatcacgccc
      961 agcaggaagt acaccagcac atgcaacacc aggccgccaa tcggcgcgcg ccccaggtcg
     1021 aagagccgct ccagccggat cttgctcagg ccggtggcga ccagcaagat ggccccgccg
     1081 acgaggaagc ggctggtcag cgtgtcgagg ggcgggacgt aggcgggggt ttgttcgggc
     1141 ggttcgccca cgcgctccag gtcggcggcg gtgtcggcgg cgaagaacca gcacaaggcc
     1201 acgatggtca ttgcgatcag catcggggcg tcgagccgcg gcaggtcctc gaagatattg
     1261 gcccgcccct cgctgatgtt ggtgctgagt tgcagcagca acatgatcac cagcgcctcg
     1321 ctgccgcggt agcgcagctt ggtcaccgtg tcccagcttc ccttcggcgc gaagcggtac
     1381 gagaaatacg attcggcggc aaccaggccg cagaagaccg ccaggtaggt gccgtcccag
     1441 gtcgggatga ccaggtgtag catctgcaca atcgccagca cgatgctgcc taccatcagc
     1501 ccgacgatga ccgggcgaaa tacatcctcg atccagcgct gcgtgctcat cgccaggtct
     1561 ccaggtcgcg gtccgaccat gcctccagca gcggcatgcc gatgcgcgca gcctgctcct
     1621 gccattcgtg ggcggcgaca tcgggctggg ccaggatcaa cgtcaccgaa aagccgcggc
     1681 gccgcaggcc cagcaggttg tcgaacaagg cctggctggc cccgccggtg atgaccacga
     1741 aggtggtgcc ccaggaggca tggacccccg cgcggtgcag caactcggcg aacgcggcgc
     1801 cctgcgtcat ctgcacgcgg gccagcgtct ccaggatatt ggtcatcagg tgggcgcgct
     1861 cggcgcgcgg cggcagcgcg atataccgct ccgcccccac cagcgggtcc accgcctcgg
     1921 tgatgaggcc cacggccagc ccctctttca aaatgatatg gctggcgatg gacgcggcgg
     1981 ccacaatcgc cagttcggtg cccggatagc gccgcgtgat ttcgtagtcg tcttcgtcca
     2041 ggtccaggca gatcagcgtc tcgcgggcga tggccggctg gtattgttta acgaccagcc
     2101 gaccggtgcg cgcggtggcg gtccagtgca ggcggcgcag ggcatcgccg cgctggtaat
     2161 cgcgcacgcc catgatgcgg ctggcatcct cgaacagcgg cgattgcacc ggcagcgcga
     2221 ccatcggcga gtgcgtgggc aggcccaact cgtgcagggc gacgacgcgc gggtagacgg
     2281 tgaggcgctt cggctcggcg ccctggccgg tgcggcgggc caggcccagt aggtcgccca
     2341 actccaggct gagcgggccg aggtcgtagt agccacggtg ccggcaggcc agcgtgtagc
     2401 ggtggtgcca ctcgccataa gggccgagcg tgatcacctg gcggtcaaac ggcacggcca
     2461 tcagccgcac cggcagcgac tcgctgacct ccacccacgg gatgggcagc cacccgccgt
     2521 tgcgcacggt cagatccacg gccaccaggt cgccggtgaa ggcatggtcg gtgaaagccc
     2581 ggcgcacacc caggtgccgg agggcgcgcc ccatccagat gcgcgacagc acatagagcg
     2641 cggccaggaa atagacgatg gtgacgaaga aatccacgcg cagggccagg gcgataacca
     2701 gcaacaccac cagcaggggg acgaagttgc gcatggcccg cgctccctat tgcttgtcgc
     2761 cgagatcgag cgtagtatgc tgcacaatat ccgcgacgat ggtccgcgcg tcgcgcccgc
     2821 gcaactggct ctccggcttg atgctgatgc ggtgcggcag ggtcagcggc gccatttcgc
     2881 gcacgtcttc gggcaccacg taatcgcggc catgcagcgc ggcgaaggcc tgcgcggtct
     2941 tgtagagggc gagcgtcccg cgcgggctgg cgccgacggc gatatcgggg tggctgcgcg
     3001 tggcccgcac gatgcccagg atatactgct ccagcgtttc gtccacgtgc acatcggtca
     3061 ccaggtcggc cagcgccggc aactcgccgc cgtccaccac ctgcccgatc tgctcgatgg
     3121 ggtgctgctt gcgcaggttc cgcagaatct gcagttcgtc cccggagccc ggatagccca
     3181 tggtcatatg gatcagaaag cggtcaagct gggcttcggg tagcggaaac gtcccctcga
     3241 actcgatggg gttctgcgtc gccagcacga cgaacggtgc gggcaggcgc cgggtcacgc
     3301 cgtccaccgt cacctgctgc tcctgcatcg cctccagcag cgccgattgc gtgcgcggcg
     3361 tggcgcggtt gatctcgtcc accagcagga agttgacgaa aaccggcccc ggctggaact
     3421 cgaaggtgtt ggcctgctgg ttatagacgc tcacgccggt cacgtcgttg ggtagcaggt
     3481 cgggcgtgca ttgcaggcgc ttgaacgcca gccccatgct ggcggccacg gcgcgggcga
     3541 gcatcgtctt gcccgtgccc ggcacgtcgt cgatcagcac atggccctgg cagagcagcg
     3601 ccaccagcac atactcgatc tgggcccgct tgccgacgat gactgtctcc acattctgga
     3661 tgacgcgctc ggcgaattgc gtcaggggcg tggtcgtcgt agtcgtcgcc atgggggatc
     3721 gtggctcctt ttgcggccca tccgctgaac gtgtgccctg cggctgcggc gttgcctctg
     3781 ctacgaggat agcatgttcc tgcggcaacg tcaatgatcc cgcccggcat cccggcagac
     3841 cggctcattc tgcaccgcga agacgcgaag gacgcgaaga atacgactaa tatcaataac
     3901 cttcgcgttc ttcgcgtctt cgc
//
LOCUS       DASUYC010000554         5652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_725175,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000554 DASUYC010000000
VERSION     DASUYC010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5652
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725175"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VF276_09075"
     CDS             complement(<1..581)
                     /locus_tag="VF276_09075"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEX6607051.1"
                     /translation="MALCEAEAAYVDAPEGALDLPHPYLAERGPGGRPRDCTDVMGVL
                     IDRTSRDQVLTQIDGFVQSGKPHQVVTVNVDFLNIAHQDRSFVRLLNAAALSVPDGMP
                     LVWLSRVLRRPLPERITGTDLILNCADLAARRGYRMFLLGAAPGVADEAAALLETRYP
                     GLRIVGTYSPPACEEFDPAENARIIERIRAARP"
     gene            955..2073
                     /locus_tag="VF276_09080"
     CDS             955..2073
                     /locus_tag="VF276_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607052.1"
                     /translation="MNHILLRAAHARVALVALALATAAALLALPATPPAQAQTPAGVL
                     APNGQRGAAFVHTTSAPFGSPASDESLKRLHDTGANWVSIITPFRQHDARAVEFYRSA
                     NDPSDSAISQMVIYAHSLGMRVMVQPIVLSDDGVWNGFFTPDDTARWFSNYRDQVAAY
                     AALAQSVQADEFCIGTEFFTLTTPQYSPQWRQVISAVRAKYFGPLTYSANWGDKKTPE
                     FATIDWWDALDYIGISAYFPLSWNNFLTDPLVEGWRHYRDPFGPNAVGQEFHWFDSVA
                     AAHVRWNKPVQFTKIGFGSYANSPGRWDLQPDPYLELSVQANAYDATMQVWGNVDWMT
                     GIFWTPWYSDPNAGGPLDNGESPQNKPAEQVLRQWYSR"
     gene            2230..3177
                     /locus_tag="VF276_09085"
     CDS             2230..3177
                     /locus_tag="VF276_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607053.1"
                     /translation="MREYLETFFRRKWLFFVPFLAALIIAVAGGLYTSWLYEVQARMI
                     VQGNPVLDGAGQQLSMPVATATNEYTALTALLQTDDFLRKVIEGVPALKGDGDPNKMD
                     ATIAKLRKDLNAWTSADSLINFRYRDRDPQTAQQVVSRTIDLFIAQRNADRVGDADRA
                     ISFLSAQQADYQQQLQQASTELSAWEQSHPPADRAKLPEAEQLNFQRLQTNYQTVLDH
                     LKYVGSELEKARFTKDKAVALQAGTYQIVDPPVVPQSPALSVNKLLGVLLVGLVVAVG
                     LGFSVVALVTWFGGRRAADSPSALPPWLNRMMAQEEQTA"
     gene            3174..3311
                     /locus_tag="VF276_09090"
     CDS             3174..3311
                     /locus_tag="VF276_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607054.1"
                     /translation="MTLILPLLFTFLLAGQFVRKWNAATIAGMTVWIAVLAGIHIFIL
                     R"
     gene            3354..4931
                     /locus_tag="VF276_09095"
     CDS             3354..4931
                     /locus_tag="VF276_09095"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX6607055.1"
                     /translation="MNDSLAFPQARGQARDTTGGAPAARPMIERPPRGVLIGLILLGA
                     LTGLAVVLTGGDLVLFGVLALIPIFGMIWYRPVFGVYLVLAGGLLLEQYGIDGLADPV
                     TGRIPLFSNIGGTEGWNAWTHENLPWLTANLLELLLAWTALALIVRERKAHRLQLGRL
                     GAPLLLFLAAVSFSWAFGLLQGGDFKVSLWEIRALFYMGICYLLTVNLVRTAGQVRIV
                     GWIVVVMIGIKALQGVYRYFVPFHGDLTGIYAITGHEDALFFDTLLLLAAGLAIYGGP
                     IRLRLLTWAVVPFTLFTLLATRRRAALFALFVGGAAYLLTLPAAKRVLLPRILVPLFL
                     LGLVYVGVFYNSDSAIAQPIQMIKSLYQPNDPRDASSNLYRDYEQYNVMSTIRHNPVL
                     GTGFGRPYEMLVPLAPIPFPLRDYIPHNEILWIWLKMGTVGFIIFWLLIASCVIQGSL
                     VMRRLKDPFFQALAGMIIALVVMQVVIAYADLQLTFYRNMIYLGVMLGILMRLDRIDP
                     VLARLRPAPAAPPPAAE"
     gene            5249..>5652
                     /locus_tag="VF276_09100"
     CDS             5249..>5652
                     /locus_tag="VF276_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607056.1"
                     /translation="MKKSRISMLLVSLSVSASMLLSACGGETATPTPATTGGGSTGAA
                     TATTASGGSTGGSGGVVKIAVDLPVSGADASDGIPTRNGVQLAIDQANKAGGVMIDGK
                     PTQLQMYFLDDVPPGGQAHDPAQGAKNADAFIA"
BASE COUNT          952 a         1946 c         1787 g          967 t
ORIGIN      
        1 tcggggcggg ccgcgcggat ccgctcgata atgcgcgcgt tctccgccgg gtcgaactct
       61 tcgcacgcgg gcggcgaata agtaccgacg atgcgcaggc cgggatagcg tgtctccagc
      121 agggcggcgg cttcgtcggc cacgccgggc gcggcgccca gcaggaacat gcgatagcct
      181 cgccgcgccg cgaggtcggc gcagttcagg atcaaatcgg tgccggtgat ccgttcgggt
      241 aacgggcggc gtagcacccg cgacaaccag accagcggca tcccgtcggg cacggagagc
      301 gccgccgcgt tcaacaggcg gacaaacgag cgatcttgat gggcgatgtt gaggaaatcg
      361 acgttgacgg tcaccacctg gtgcggcttg ccggattgca caaatccgtc gatctgagtt
      421 agaacctggt cccgcgacgt gcgatcgatc agcacgccca tcacatcggt gcaatcccgc
      481 gggcggccgc cggggccgcg ctcggcaaga taggggtggg ggagatccag tgccccctca
      541 ggggcgtcca cgtaggcagc ctcggcttcg caaagggcca tgcgagcctc cttcatgaag
      601 gtgaaatagt gcttgttaga gcgaaaagta gaaagacgga aacgatctcc ggctccgttc
      661 cggcggtcgc tgggccatcg cgccgcggca ggaacagagt cctgtgcgcc ccggcatcca
      721 cgggccgccg tgccctggga tgcctatctc gcttcagcca actggctacc caagcatgaa
      781 ttacgtggca caaccacaag aggaggtagc cgttacaggc acataggcaa atttagtgcc
      841 aaggaacgat gtttcgcgcg cacacccgcg taacgatggg cgcgcccggc ttcgttgtag
      901 tgagtaagca gtaggacgtt ggctctgcaa cacagagagg aaccagataa ccgtatgaat
      961 cacatcctgt tgcgggcggc gcatgcccgc gtggccctgg tagccctggc actggcgacc
     1021 gccgccgccc tgctggccct gccggccacc ccgcctgccc aggcccagac gcccgccggg
     1081 gtgctggcgc ccaacggcca gcgcggcgcg gcgttcgtcc atacgaccag cgcgcccttc
     1141 ggctcgcccg cctcggacga atccctgaag cggctccacg acaccggcgc caactgggtc
     1201 tcgatcatca caccattccg ccagcatgat gcccgcgccg tcgagttcta ccgctcggcc
     1261 aacgacccca gcgacagcgc catctcccag atggtcatct acgcccattc gctgggcatg
     1321 cgcgtcatgg tccaacccat tgtgctctcc gacgacgggg tctggaacgg gttcttcacc
     1381 ccggacgaca ccgcgcgctg gttctccaac tatcgcgacc aggtggcggc ctacgcggcg
     1441 ctggcccaga gcgtgcaggc cgacgagttc tgcatcggca ccgagttctt tacgttgacc
     1501 acgccgcaat actcgccgca gtggcgtcag gtgatcagcg ccgtgcgcgc gaaatacttc
     1561 ggcccgctga cctactcggc caactggggc gacaagaaga cgcccgaatt tgccactatc
     1621 gactggtggg acgcgctcga ctatatcggc atctcggcct atttcccgct ctcctggaac
     1681 aacttcctga ccgatcccct ggtcgagggc tggcggcact atcgcgaccc gttcggccct
     1741 aacgccgtcg gtcaggaatt ccactggttt gactcggtgg ccgccgcgca cgtccgctgg
     1801 aacaagccgg tgcagttcac caaaatcggc ttcgggtcgt atgccaactc gccgggccgc
     1861 tgggatctgc aacccgaccc ctacctcgaa ctgagcgtgc aggccaacgc ctatgacgcg
     1921 acgatgcagg tctggggcaa cgtcgactgg atgacgggca tcttctggac gccgtggtac
     1981 agcgacccga atgccggcgg cccgctggac aacggcgaaa gcccgcaaaa caagcccgcc
     2041 gagcaggtgc tccgccagtg gtacagccgc taagcgcggc ggcgggggcc gtggaacggt
     2101 tcctgcttat gcacgggcgg tgccggtctc aggcgaccgg cgccgccagg gcgcactacc
     2161 cgcacataac cccattccac tggcgggccc ccggatgcct ggttggttgg tgaaaggatc
     2221 aatcgaacga tgcgtgaata tttggaaacc ttcttccggc ggaagtggct cttcttcgtg
     2281 ccgttcctgg cggcgctgat tatcgccgtc gccgggggcc tctatacgtc ctggctgtat
     2341 gaggtgcagg cgcgcatgat cgtgcagggc aaccccgtgc tcgacggcgc cgggcagcag
     2401 ttgagcatgc cggtcgccac cgcgacgaac gagtacaccg ccctgacggc gttgttgcaa
     2461 accgacgact tcctgcgcaa ggtcatcgag ggggtaccgg ccctcaaggg cgacggcgat
     2521 ccgaataaga tggacgccac catcgccaag ttgcgcaaag acctgaacgc ctggacttcc
     2581 gccgacagcc tgatcaactt ccgctaccgc gaccgcgacc cgcaaaccgc gcagcaggta
     2641 gtcagccgga cgatcgacct gttcatcgcg cagcgcaatg ccgaccgggt gggcgacgcc
     2701 gatagggcga tcagcttcct cagcgcgcag caagccgatt accagcagca actccagcaa
     2761 gcctcgacgg agctgtcggc ctgggaacag agccacccgc cggccgaccg ggccaagctg
     2821 cccgaagccg agcagttgaa cttccagcgc ctgcaaacca attaccagac cgttctcgac
     2881 cacctcaagt acgtgggatc ggagttggaa aaagcgcgct tcaccaagga caaggccgtg
     2941 gccttgcagg cggggaccta ccagatcgtg gacccgccgg tggtgccgca aagcccggcg
     3001 ctgtcggtga acaagctgct cggggtgctg ctggtcggcc tggtggtggc cgtgggcctg
     3061 gggttcagcg tcgtggcgct ggtaacgtgg ttcgggggcc ggcgcgcggc ggacagcccc
     3121 tcggcgctgc cgccctggct caaccgtatg atggcgcaag aggagcagac ggcgtgacgc
     3181 tgatcctgcc gctactcttc accttcctgc tggccgggca attcgtccgc aagtggaacg
     3241 ccgcgaccat cgccgggatg accgtctgga tcgccgtcct cgccggcatc cacatcttca
     3301 ttttgcgtta gtgaccgcgg gtgccgcgcg gcgcccgcca tgattggaca gcgatgaacg
     3361 actccctggc cttcccccag gcgcgcgggc aagcccgcga caccaccggc ggcgcgcccg
     3421 ccgcccgccc catgatcgag cgcccgcccc gcggcgtgct gatcggcctg atcctgctgg
     3481 gcgcgctgac cgggctcgcc gtggtgctca caggcggcga cctggtgctg ttcggcgtgc
     3541 tggcgctgat ccccatcttc ggcatgatct ggtaccggcc cgtgtttggc gtctacctgg
     3601 tgctggccgg ggggctgctg ctcgaacagt acggcatcga cggcctagcc gacccggtga
     3661 cggggcgcat cccgctgttc agcaatatcg gcggcaccga gggctggaac gcctggaccc
     3721 acgagaacct gccctggctg acggccaacc tgctggaact gttgctcgcc tggaccgcgc
     3781 tcgcgctgat cgtccgcgaa cgcaaggccc accggctgca gctggggcgg ctgggcgcgc
     3841 cgctgctgct gttcctggcg gcggtcagct tttcgtgggc gttcggcctg ctccagggcg
     3901 gcgacttcaa ggtctcgctc tgggagatcc gggcgctgtt ctacatgggc atctgctact
     3961 tgctgaccgt caacctcgtg cggaccgccg ggcaggtgcg catcgtgggc tggatcgtcg
     4021 tcgtcatgat cggcatcaag gcgttgcagg gcgtgtaccg ctacttcgtg cccttccacg
     4081 gcgacctcac cgggatttac gccatcaccg gccacgagga cgcgctgttc ttcgacacgc
     4141 tgctgctgct ggccgccggt ctagctatct acggcggccc catccgcctg cggctcctca
     4201 cctgggccgt agtgccgttc acgttgttca cactgctggc gacgcgccgc cgggccgccc
     4261 tcttcgcgct gttcgtcggc ggcgcggcct acctgctgac cctgcccgcc gccaagcggg
     4321 tgctgctgcc gcgcatcctg gtgccgctgt ttctactcgg cctggtctat gtcggggtgt
     4381 tctataacag cgactccgcc atcgcccagc ccatccagat gatcaagtcg ctctaccagc
     4441 ccaacgaccc gcgcgacgcc tcgtccaacc tgtatcgcga ctatgagcaa tacaacgtta
     4501 tgtcaacgat acgccacaac cccgtactgg gtaccggctt cgggcggcct tacgagatgc
     4561 tggtgccgct ggcgccgatc cccttccctc tgcgtgacta cattccgcat aacgagatcc
     4621 tctggatctg gctgaagatg ggcacggtgg ggttcatcat cttctggctg ctcatcgcat
     4681 cgtgcgtgat ccagggcagc ctggtcatgc ggcggctgaa agatcccttc ttccaggcgc
     4741 tggccggcat gatcatcgcc ctggtggtga tgcaggtcgt gatcgcctat gccgacctgc
     4801 aactcacctt ctaccgcaac atgatctacc tcggcgtgat gctgggcatc ctgatgcggc
     4861 tggatcggat cgatccggtg ctagcccgcc tgcgccccgc gcccgcggcc ccgccgcccg
     4921 ccgccgaata gcgggcgccc tcagcatacg ctacatcccg gccccggatg gggagaatcc
     4981 cttgcgtcca tcttgacaag cggcccgcgc cccactataa tgcctgcaaa gttggccgat
     5041 aggcccagga cgggggtctg tcgccgtgtc ccgcaccgtc catgctagag gggaggtgtg
     5101 gcgcccgttc gtttccggtt cgccggttct cacgaaactt cgttctaccc ttcctgtaaa
     5161 cacctgccgg gccagggcaa cccgtcgcag tggaacccca cgagaggttt ttcgtatccc
     5221 tagcacaatc gcatggagga gagcttctat gaaaaaatcc cggattagca tgctgctggt
     5281 ctcgctgagc gtgagcgcga gcatgctgtt gagcgcgtgc ggcggcgaga cggccactcc
     5341 caccccggcc accaccggcg gcggcagcac cggcgcggcc accgccacca ccgccagcgg
     5401 cggcagcacc ggcggctcgg gcggcgtggt caagatcgcg gtcgacctgc cggtgtctgg
     5461 cgccgacgcc agcgacggca tccccacccg caacggcgtc caactcgcca tcgaccaggc
     5521 caacaaggcc ggtggcgtga tgattgacgg caaaccgacc cagctccaga tgtacttcct
     5581 tgatgacgtg ccccccggcg gccaggcgca cgacccggcc cagggcgcca agaacgccga
     5641 cgccttcatc gc
//
LOCUS       DASUYC010000555         3060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1221477, whole genome shotgun sequence.
ACCESSION   DASUYC010000555 DASUYC010000000
VERSION     DASUYC010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221477"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..974
                     /locus_tag="VF276_09105"
     CDS             60..974
                     /locus_tag="VF276_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607057.1"
                     /translation="MKRLSLVLATALFAALLGLLAACGETPTPTPVPTPTPTPLPPTN
                     TPAATGPTGGSPASASDIALINDAITKTAGVESYHFDIHLTVPGMLPNTAITGDYKSP
                     DKAHVFITSTTGTQELINIGTTSYVKQADGTWVSQDLNAGAGSDTGSMGSLGGLSSMA
                     GGLDPTKATNIFGMLARFATGVTSATVIGPDPGKGTHYQVPIYLGNLMGTGGSTTPGE
                     TPLGHADIWIDPADGLIHSMMIKLDLTDLVQAFAGLGPTAVPGAPTATPIPPIVIDMQ
                     AQLSNFGQVGDITAPAGARPAPGGTATP"
     gene            1146..1610
                     /locus_tag="VF276_09110"
     CDS             1146..1610
                     /locus_tag="VF276_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607058.1"
                     /translation="MDKNFVVGKLNEMLVLEQGIIEVERTLARKSRNADIRTITDLAA
                     REDETHALALTRMIRDLGGQVQPPPMEGRAWIEALIRAINVERDELDRLGLYRMLKFR
                     AVATGEVMDVLRRALGNPPPMESLIIMLHQDREHAAQLARIESTYAANEAVL"
     gene            1835..2359
                     /locus_tag="VF276_09115"
     CDS             1835..2359
                     /locus_tag="VF276_09115"
                     /inference="COORDINATES: protein motif:HMM:NF022734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HEX6607059.1"
                     /translation="MPLQEIDLGPVLGLQAESVGVPGQRTFRIHAHSAQGAARLWLEK
                     EQLQALAVAIEEFISELPAARRTLPGSSASLPFPPQATLEFTIGRLALGYDPENDLIS
                     LIVRELTDDPDRETGQALRCQANREQMQALSRQALEVVAAGRPRCPLCNTPLTGPTHW
                     CPRSNGHAPLPTEG"
     gene            2367..>3060
                     /locus_tag="VF276_09120"
     CDS             2367..>3060
                     /locus_tag="VF276_09120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX6607060.1"
                     /translation="MAPREGRAYRDALAYLYSFTDVERVPGLAPAAGFDLAKVHRFFD
                     LLGRPETAYHSILVAGTKGKGSTANYLAGALRAAGYRVGLYTQPHLTSFRERMQVDGA
                     LLPADRLGPLVESIRPVVERMHAEAPEYGPLTTYEIATGLALRYFAEAQVDYAVLEIG
                     LGGRLDAVNAVRVPLVSVITSLSLDHTRVLGDTIDKIAYEKAGIIKPGIPVVTAPQPP
                     DALPVIVNTARER"
BASE COUNT          542 a         1080 c          994 g          444 t
ORIGIN      
        1 tggcgaaaag ggcgcgggct acctgcgccg gatactgaac gcgaaaagga gccggatcca
       61 tgaaacgtct ctccctggtc ctggcgacgg cgctgttcgc ggcgctgctg ggcctgctgg
      121 ccgcctgcgg cgaaacgccc acgccgacgc ccgtccctac cccgacgccg acccccctcc
      181 cgccgacgaa cacaccggcg gccacgggtc cgaccggcgg cagccccgcc tcggcaagcg
      241 atattgcgct gattaacgac gccatcacca agaccgcggg agtcgagagc taccacttcg
      301 acattcacct gaccgtgccc ggcatgctgc ctaacacggc catcaccggc gattacaaga
      361 gccccgacaa agcccatgtg ttcatcacga gcacgaccgg cacgcaggaa ctgatcaaca
      421 tcggcacgac gagttatgtg aagcaagccg acggcacctg ggtcagccag gacctgaacg
      481 ccggcgcggg cagcgacacc ggatccatgg gcagcctggg cggcctgagc tcgatggccg
      541 ggggcctcga cccgaccaag gccaccaaca tcttcggcat gctggcccgc tttgccaccg
      601 gcgtcacctc ggccacggtg atcggcccgg accccggcaa gggcacacac taccaggtgc
      661 ccatctacct gggcaacctg atgggcaccg gcggcagcac cacgccaggc gaaacaccgc
      721 tcggccacgc cgacatctgg atcgacccgg ccgacgggct gatccacagc atgatgatca
      781 aactcgacct gacagatctc gtccaggcgt tcgccgggct ggggccgacc gcggtgcccg
      841 gcgcacccac agccacgcct atcccgccca tcgtcatcga tatgcaggcc caactcagca
      901 atttcggcca ggtgggcgac attaccgccc cggcgggcgc gcgcccggcc cccggcggca
      961 ccgcgacgcc ctaaagacgg cggcgcgggc cgttgcaagc gccgcgcggc cccgttcttt
     1021 gttgtgacat ctggtataat gcttgcggat tgcgtggcgg cccaccctgg cagccgaggc
     1081 gcacgggcac catccgggtg ggcgaaggtc cggatatcag ggaggaggtc gtagtcttca
     1141 gcagtatgga taaaaacttt gtcgtcggta aactgaacga gatgctcgtc ctggagcagg
     1201 gcatcatcga ggtcgagcgg acgctcgcgc gcaaatcgcg caacgccgat attcgcacta
     1261 tcaccgactt ggcggcgcgg gaagatgaaa cccacgcgct ggccctcacg cggatgatcc
     1321 gcgacctggg cgggcaggtt cagccgccgc cgatggaggg ccgggcctgg atcgaagccc
     1381 tcattcgcgc catcaacgtg gagcgcgatg aactggatcg gctggggctg tatcgcatgc
     1441 tcaagttccg cgcggtggcg accggggaag tgatggacgt gctgcgccgg gcgctcggca
     1501 acccgccgcc catggagagc ttgatcatca tgctgcacca ggaccgcgaa catgccgcgc
     1561 aactcgcccg catcgagtcc acgtatgccg cgaacgaggc cgtcctgtaa gggatacccg
     1621 gccatttgac cgcaccgcag ggcgcacccg ctgatgcgcc gctgcccgca cccgcccgcc
     1681 ggctggagcc ggcgggcctt tgtttgcgcc cccgccaatt cgtgctatac taatgtgggt
     1741 tggcctgatt cgcgcggcac agcgtttgca gtatttcaca gcgagagaga atccgtcgcg
     1801 gcccggcgcc cggacggaat tagaggacgg agcaatgcca ctgcaagaga ttgacctggg
     1861 tccggtgttg gggttacaag ccgaatcggt gggcgtcccc ggccagcgca ctttccgcat
     1921 tcacgcccac tcggcacagg gcgcagcgcg cctctggttg gagaaggagc aactccaggc
     1981 gctggcggtt gccatcgagg aattcatctc ggagctcccg gcggcgcggc gcaccctgcc
     2041 cggcagcagc gcatccctgc ccttcccgcc gcaggccacg ctcgaattca ccatcgggcg
     2101 gctggcgctg ggctatgatc cggagaacga cctgatcagc ctgattgtgc gtgaactgac
     2161 cgacgatccc gaccgcgaga ccggccaggc gttgcgctgc caggccaacc gcgaacagat
     2221 gcaggcgctg agccggcaag cgctggaagt cgtggccgcg ggccggcccc gctgccccct
     2281 ctgcaatacc ccgctgaccg gtccgacgca ctggtgcccg cgcagcaacg ggcacgcgcc
     2341 gctgcccacc gaaggctagg atcagcgtgg cgccgcgcga gggccgggcc taccgggatg
     2401 ccctggccta cctgtacagc ttcaccgatg tcgagcgcgt gccggggctg gccccggcgg
     2461 cgggcttcga cctggcaaag gtccaccgct tttttgattt gctggggcgg cccgagacgg
     2521 cctaccacag catcctcgtg gccggcacca aaggcaaagg ctcgaccgcg aattacctgg
     2581 ccggagcatt gcgcgcggcg ggctaccgcg tggggctcta cacccagccc cacctgacca
     2641 gctttcgcga gcgcatgcag gtggacggcg ccttgctgcc cgcagaccgg ctggggccgc
     2701 tggtcgaaag catccgcccg gtggtcgagc gcatgcacgc cgaggcgccc gaatacggcc
     2761 cgttgaccac gtacgagatc gccaccgggc tggcgttgcg ctacttcgcc gaagcccagg
     2821 tggactacgc cgtgctcgaa atcgggctgg gcgggcggtt ggacgccgtc aatgccgtgc
     2881 gggtgccgct cgtgtcggtc atcacctcgc tcagcctcga ccacacgcgg gtgctcggcg
     2941 acaccatcga caagatcgcc tacgaaaagg ccgggattat caagcccggc atcccggtgg
     3001 tgacggcgcc ccagcccccc gacgcgctgc ccgtgatcgt gaacaccgcc cgcgaacggg
//
LOCUS       DASUYC010000556         2832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_75012,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000556 DASUYC010000000
VERSION     DASUYC010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75012"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..1089
                     /locus_tag="VF276_09125"
     CDS             7..1089
                     /locus_tag="VF276_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004422771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607061.1"
                     /translation="MERAAANNAISGYNCGSPGEPCDGRSRPYFRPGSSVTRAQASKM
                     IDLARRGGVPPTPQPTQPPPPPPPGGGATIGGCPLFPADNIWNRAVNTLPVHPNSAGY
                     INSIGGGASLHPDFAAGSWNGAPIGIPVNVVPATQPLVPLNLGQWAGVSDPGPYPIPP
                     NAAIEGGSGDRHVLVVQSGTCLAVELYHADPAGGGAWNADTAARWSLTSNALRPNGWS
                     SADAAGLPILSGLVYYDEVATGAIHHAIRFTTANIGQSYVWPARHSDGDSTDPNVPPM
                     GTRLRLKASVDISGFDGKTQVILRALQTYGMILADTGGPLNISGAPDGRWNDELLNAM
                     EAVHASDFEAVDTSSLMIDPNSGQSR"
     gene            complement(1279..1851)
                     /locus_tag="VF276_09130"
     CDS             complement(1279..1851)
                     /locus_tag="VF276_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007938290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX6607062.1"
                     /translation="MYKTICVYSSSSDAIAPEFFAVAEELGAALAGAGYGLIYGGARV
                     GLMGAVARAVHAHGGHVVGVLPGVLRRKELTYEPADELIITQDLRERKAIMETRADAF
                     IALPGGFGTLEEVLEILTLKQLLLHSKPLVLLNTRGFYAPLEHLFEHIYRERFARPES
                     RQLYHFAADVPAALAYLTAYTPPQPPGKWF"
     gene            2009..>2832
                     /locus_tag="VF276_09135"
     CDS             2009..>2832
                     /locus_tag="VF276_09135"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HEX6607063.1"
                     /translation="MDTLTLAAFLFVLGAAGAGMWLVRRARRASAMTAPAPAPATLPV
                     RPPAPLPLAMDPASPFQEPLALVAANLAARPAPVDEKTRPAVFAMRIAGAAISQGAPP
                     PGIPSATTAPPPPNASGPRVVLGSTYLQTNRMRRLTSFQVGKDGEDRIAARISGLLDT
                     QWYMFRNLVLPDRKGDIDIVLVGPGGIWALEVKAYTGNYRVENGRWYKETSNGRTARK
                     SNGPGHQVRGNAARLCNFLKENGVLENNSVQPVVVLARDLPIDVVSSGTAIWLLDTL"
BASE COUNT          453 a         1010 c          933 g          436 t
ORIGIN      
        1 cagtttgtgg aacgcgccgc ggccaacaac gccatcagcg gctacaattg cggcagcccc
       61 ggcgaaccgt gcgacggtcg cagccggccc tatttccggc ccggctccag cgtgacccgc
      121 gcccaggcga gcaagatgat cgacctggcc cggcgcggcg gggtgccgcc caccccgcag
      181 ccgacgcagc cgccgcctcc tccgccgccc ggcggcggcg cgaccatcgg cgggtgcccc
      241 ctcttcccgg cggataatat ctggaaccgt gccgtcaaca cgctgccggt ccacccgaac
      301 tcggccggtt atattaactc catcggcggc ggcgccagcc tgcaccccga cttcgccgcg
      361 ggcagttgga acggcgcgcc catcggtatc cccgtgaacg tggtgcccgc cacgcagccg
      421 ttggtgccgc tgaacctggg ccagtgggcg ggcgtaagcg accccggtcc ctacccgatc
      481 ccgcccaacg cggccatcga gggcggcagc ggcgaccggc acgtgctggt ggtccagagc
      541 ggcacctgcc tggccgtcga actctaccac gccgatccgg cgggtggggg cgcctggaac
      601 gccgataccg ccgcccgctg gtccctgacc tcgaacgcgc tgcggcccaa cgggtggagt
      661 tccgccgacg ccgccggcct gcccatcctg tccggcctgg tgtactatga cgaggtggcg
      721 accggcgcga tccaccacgc tatccgcttc accacggcca acatcggcca gagctatgtc
      781 tggcctgccc gccacagcga cggcgattcg accgacccga acgtcccgcc gatgggcacg
      841 cggctgcggc tcaaggcgag cgtcgatatc agcggctttg acggcaagac ccaggtgatc
      901 ctgcgcgcct tgcagaccta cggcatgatc ctggccgata ccggcggccc gctcaacatc
      961 agcggcgccc ccgatggacg ctggaacgac gaactgctga acgccatgga ggcggtgcac
     1021 gcctcggact ttgaggcggt ggatacctcc agcctgatga tcgatcccaa ctccggccag
     1081 agccgttagc cgcgagcgac ctgccggtcc cattacacag gaagcgggct gttcccctcg
     1141 ggggcagccc gcttcgttct gttaacggcg atccgattct ctgtaggggc cggtttgaaa
     1201 ccggcccgcg ccccgctcct cggtgtaggg gccggtttga aaccggcccg cgccccggcc
     1261 ctatgcccta atggccgcct aaaaccactt ccccggcggc tggggcggcg tgtaggcggt
     1321 caggtaggcc agggcggcgg gcacgtcggc ggcgaagtgg tacaactggc gcgactcggg
     1381 ccgggcgaag cgctcgcggt agatgtgctc gaagaggtgt tccagcgggg cgtagaaccc
     1441 gcgcgtgttc agcagcacca gcggcttgct gtgcagcagc agttgcttga gcgtcaggat
     1501 ctccagcacc tcctccagcg tgccgaagcc gccgggcagg gcgatgaagg catcggcccg
     1561 cgtctccatg atcgccttgc gctcgcgcag gtcctgggtg atgatcagtt cgtcggcggg
     1621 ttcgtaggtc agctccttgc gccggagcac gcccggcaac acgccgacga cgtggccgcc
     1681 gtgggcgtgc accgcccgcg ccaccgcgcc catcaacccc acccgcgccc cgccgtagat
     1741 cagcccgtag ccggccccgg ccagcgccgc gcccaactcc tcggccacgg cgaagaactc
     1801 cggcgcgatg gcgtcgctgg acgagctata gacgcatatc gtcttgtaca caaaggccct
     1861 cctgtttggg actaatgggc tacgccggat cgggagcaaa gtaccttcaa gtccggtgcc
     1921 ggtcctatta tactgctaag gcaagccgca acgcattgcc ggcggctgcc gatttcgacc
     1981 tgatcaccgc tatagaagac aggggcatat ggatacgcta acgttggctg ctttcctgtt
     2041 tgtgctgggc gcggcgggcg ccgggatgtg gctcgtgcgc cgtgcccgcc gcgcgtccgc
     2101 tatgaccgcg ccggcgccgg cgccggcgac actgccggtg cgcccgcccg cgccgttgcc
     2161 gctcgctatg gacccggcat ccccgttcca ggaaccgctg gcgctggtgg ccgcgaacct
     2221 ggccgcccgc cccgctccgg tggatgagaa aacccgcccg gcagtatttg cgatgcgcat
     2281 cgccggggcg gcgatctccc aaggagcgcc cccgccgggg atcccgtctg cgaccaccgc
     2341 gccgcctccg cccaatgcgt ccggcccgcg cgtcgtcctg ggcagcacct atctgcaaac
     2401 caatcggatg cgccggctga ccagcttcca ggtgggcaag gatggggaag accgcatcgc
     2461 ggcccgcatc agcggcctgc tggacaccca gtggtacatg ttccgcaacc tggtcctgcc
     2521 ggaccgcaag ggcgacatcg acatcgtgct ggtcggcccc ggcgggatct gggcgctgga
     2581 agtgaaggcg tatacgggca attaccgggt ggaaaacggc cggtggtaca aggaaaccag
     2641 caacgggcgt accgctagga agtccaatgg gccgggtcac caggtgcggg gcaatgccgc
     2701 caggctatgc aatttcctga aagagaacgg cgtcctggag aataactccg tgcaaccggt
     2761 ggtcgttcta gcgcgggatc tgcctatcga cgttgtgtct tccggcaccg cgatatggct
     2821 cctggacact ct
//
LOCUS       DASUYC010000557         2144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1648716, whole genome shotgun sequence.
ACCESSION   DASUYC010000557 DASUYC010000000
VERSION     DASUYC010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1648716"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VF276_09140"
     CDS             <1..282
                     /locus_tag="VF276_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607064.1"
                     /translation="PALAAAGRWRQAEARERAALAEVRAAVRWPTHLDRWQPDLWPDV
                     PAPGVARPRPPIAALRSEVRALRRHLPYAGLFLGSLAGPVLLAGLLAGV"
     gene            complement(299..400)
                     /locus_tag="VF276_09145"
                     /pseudo
     CDS             complement(299..400)
                     /locus_tag="VF276_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            481..795
                     /locus_tag="VF276_09150"
     CDS             481..795
                     /locus_tag="VF276_09150"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEX6607065.1"
                     /translation="MTNKDISSGVVHEVPADLREALISDPEALAVWEDLTPLARNEWI
                     CWTISVKKPETRRQHVERVCTELKEGMRRPCCWYGCVHRTDKAVSPSVQKMVLDRRPK
                     SE"
     gene            1407..>2144
                     /locus_tag="VF276_09155"
     CDS             1407..>2144
                     /locus_tag="VF276_09155"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEX6607066.1"
                     /translation="MCGIFGYAGPNCEAADLVFSGLKELEYRGYDSWGIALDKDGQVV
                     TVKNTGKLRAAPEGMGQSALAIGHTRWATTGAVTHDNAHPHSDCSGRLAVVHNGIVEN
                     YVELKEELIARGHQFKSETDSEVIAHLLEEEIAAAPGDSLAEALRRVSLLLHGMNAVV
                     AMDVTRAALAAVKNGSPLVIGVDEGANYLASDIAALLKHTRHIIWVLDGQVASISKNH
                     VSLVDAATGAPIETRIERVAWDAQQAAL"
BASE COUNT          397 a          705 c          657 g          385 t
ORIGIN      
        1 cccgcgctgg ccgccgccgg gcgctggcgg caggccgagg cgcgcgaacg cgctgccctg
       61 gcggaggtgc gcgccgcggt gcgctggccc acccatctgg atcgctggca gcccgatctc
      121 tggcccgacg tgcccgcgcc cggcgtcgcg cgcccgcgcc cgcccatcgc cgcgctgcgc
      181 agcgaggtcc gcgccctgcg ccgccacctg ccctacgccg gcctcttcct cggcagcctg
      241 gccgggccgg tgctcctggc gggcctgctg gccggcgtct agagcctgtt caggccggca
      301 gcacaaagcc ccgtttggta tccggcagtt tcaccacttc cagccgaatc ccaggtccag
      361 cagccggctg ttcagcgttc tcgccggcgt atccctggtc tcctgatcga caaacggcac
      421 ttccaccgtt tcgcctatta ccgggtatat cttacggaag gtttgtgtta taatggccct
      481 atgaccaaca aggatatttc cagcggcgta gtacacgaag ttccagcaga tttacgggaa
      541 gctttgatat ctgacccgga agcgctcgca gtctgggagg atcttacgcc ccttgcccgc
      601 aatgaatgga tatgctggac gatctcggtt aaaaagccgg aaaccagaag gcagcatgtt
      661 gaaagggtgt gtacagaact taaagaggga atgcgccgcc cttgttgttg gtatggctgt
      721 gttcaccgga cagataaggc agttagccct tcagtacaga aaatggtgct tgatagacga
      781 cctaagtccg aatagcttgt ttgccgccaa gaagtgcata acaggctcta gtcctgcccg
      841 gcggcgcaga caactccggc acccgcatcc tcccctgctt accctaacct gatgaagttc
      901 cctaatattc agaaagcccc gcgcgccagt ttctgacagg tcatccacaa actattgtat
      961 cgcggcgcgg ggtttgctat actccgatct gtggcagggc ggcaacgcgg ccacacgaga
     1021 acagcgaaat cggatcggag acaaacaccg caggggtttg acaactagac agtagcgcct
     1081 ggactcgccg cggaggcggt gagttgacga ggtgggagct tatcgaaaga ttcggcggat
     1141 ggctccccgg ttccacaatc gtgagagacg gccaaatcgc gtgagtgatt gcgcgacaac
     1201 acccggctca agtggcggag cagcatgctc ccggcctttc cccctggttg tgttccattt
     1261 cccggtttct cctgctgtgg ggttagtgtg ccccggacga cgcgtgcgcc tgtcccggtg
     1321 gcccgacccc cgccctgcgc cctgtcccgg atgagtccga ctggactcac cccgtcgctt
     1381 gctgtgtcat cgaaggagta ttgtgcatgt gtggtatctt cggctatgcc ggtccgaatt
     1441 gcgaagccgc cgatctcgtc tttagcggat tgaaggaact cgaatatcgc ggctacgact
     1501 cgtggggcat cgccctcgat aaggacggcc aggtcgtcac cgtcaaaaac accgggaaac
     1561 tccgcgccgc gcccgaaggc atgggccaat cggcgctcgc catcgggcac acccgctggg
     1621 ccactaccgg cgccgtcacc cacgacaacg cccacccgca cagcgattgc tcgggccgcc
     1681 tggccgtggt ccataacggc attgtcgaga attatgtcga gttgaaggag gagctgatcg
     1741 cccgcgggca tcagttcaag tcggagaccg acagcgaggt catcgcccac ctgctcgaag
     1801 aagagatcgc cgcagccccc ggcgacagcc tggccgaggc gctgcgccgc gtcagcctgc
     1861 tgctgcacgg catgaacgcc gtggtcgcca tggacgtaac ccgcgccgcg ctggccgcgg
     1921 tcaagaacgg gtcaccgctg gtgatcggcg tggatgaagg cgccaactac ctggcctccg
     1981 acatcgccgc cctgctcaag cacacccgcc acatcatctg ggtgctggac ggccaggtcg
     2041 cgagcatcag caagaaccac gtcagcctgg tggacgccgc gaccggcgcg cccatcgaga
     2101 cgcgcatcga gcgggtggcc tgggacgcgc agcaggcggc cctc
//
LOCUS       DASUYC010000558         9128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_809848,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000558 DASUYC010000000
VERSION     DASUYC010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 9128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9128
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809848"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..901
                     /locus_tag="VF276_09160"
     CDS             59..901
                     /locus_tag="VF276_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607067.1"
                     /translation="METESPHDQDWFWEGNIQARVLDYMQNEEGFTILSPGEAMPAEQ
                     GIEIVAERAAGERTVHRLVSVRGWPSAIHRRGTMAGRPRSTRPEVIARSWVAQAVLDL
                     ALGRNGDPDIDLCLALPAVASYIRYIQRLRWFLAVGRVTVYLVSQEGRVAVTPPGAPP
                     VSHFAPSPPPAIPGQRRKLGLPGASRMQLPLLHALISAGGAAGRAELIPLVARWFPEV
                     PQPPPAEFGQRLSVAQRGLQVDGLTELDGRGVWRITEAGRTLYAAQWEDWRAKQERQA
                     APDR"
     gene            complement(898..2169)
                     /locus_tag="VF276_09165"
     CDS             complement(898..2169)
                     /locus_tag="VF276_09165"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6607068.1"
                     /translation="MDPSPAPPPARRGPFAGLTRNVFVLGIVSLFTDISSEMIVPVRI
                     LFLVGVLNTPLILAGLIEGLAESTVSILKIISGRMSDRVNSRKPLITVGYGLSNGIKP
                     FLALAMTWPQALGFIFLDRVGKGVRGSPRDALLADSTDAQNRGKAFGFHRSMDTLGAA
                     IGPLLTVGILALNNQDLRQVFAWTAVPGVLGVLALVFLREQRRERPATPAKAADQPKV
                     PWQQQVRALGPRFWMFTFISTIFALGNSSDAFLFLRAEGLESALEAVPLVYFGYNIIY
                     AVLATPLGVLSDRWGRAPVLIAGYLSFALVYAGWTVANQSWHVWALFAVYGIYAAATD
                     GVSKALIADVIPKAQRGTAMGWFNGVTGFAALPANVLGGWLWSTFGPGATFGFGAWAG
                     FVAAALMIAWLPWLLGKRSLGPARASEAPAG"
     gene            2397..3791
                     /locus_tag="VF276_09170"
     CDS             2397..3791
                     /locus_tag="VF276_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HEX6607069.1"
                     /translation="MASTARTTSDTPARDTTLAPPARRVGRILSWVFLIALAALLAIS
                     NPILVNLQPRFDLALLIPAAGVILAALGARRGWPLLIWPGAAVLWLGIVACITTQSSA
                     IGTAPALVWGERGIVLVGAGLIWFLLLGLPGWVQRAALAAALPTLVIFAVLWITAPTV
                     VRTAGFYWLAADARGRLYATDIDNGSIWVFDWNGNVSGQIWPRRAAPGSPGPGIQPAG
                     MGSEFTQQIARTTPKDLPLDRTFHTCGMAVDPENYLYVLDTGLSRIQQFNPDGHLRAT
                     WPLPQTYISAPGCLAADATRLYIADNRGIILVYDHAGVQQTEWKLSGPSRDLAVGWDG
                     RLYVLHDASVEVLEIAGGRHLLQWDLPPGIGTVPYDMILARRNGEILINNNLTAQVLR
                     YRPSGEALPALGKPGTEPGQFGVPAGLTEDAESRLYISDAAYRVIQRFSPSYQVEAVW
                     SVPEEEKDEAERRR"
     gene            complement(3867..4793)
                     /locus_tag="VF276_09175"
     CDS             complement(3867..4793)
                     /locus_tag="VF276_09175"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6607070.1"
                     /translation="MELTMRRYHDDGDYWRIRQFLRAVAPGNRAWDLNWAVYRFDYCR
                     WHAWENIEHPWLPDAVFLWETADGRLAAVVHPEGKGEAFLEVDPVLRTPALEAAMLAR
                     AEAQLADTGRDGRRRLAVWVNEHDNLRQQVLRSRGYRQDSGPEYQRRRSLDLPILQTA
                     PGAGYTVRALGDREELPARSWASWRAFHPDEPAGRYRGWEWYENVQRAPLYRRDLDLV
                     AVAPGGEIAAFCTVWFDDVTRAAAFEPVGTAPEHQRRGLARAVMSEGLRRAQHLGATV
                     ATVGSYSPSAHALYAAIGFTDYALSYPWVREW"
     gene            complement(5132..6874)
                     /locus_tag="VF276_09180"
     CDS             complement(5132..6874)
                     /locus_tag="VF276_09180"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607071.1"
                     /translation="MDTTVRRASPARRARFLILAALLAAVSLAGTLPAPAAAQSGPND
                     TLTNEQLVAQAVSNMQGLASYHFEFRGESYAQGDAERESLTIAANLQGNANGSRVTMR
                     YAPGVARDETQSTLSIGQYIAPATEVRFTDAEWRESYDGGKTWSRPETDTPAAYLMVG
                     VVCVWRGHCWQPGATSAGSSIISRGLANPVIADGSPRTQAVDGVPTRHLVGEFSEAAG
                     FPKGTPVELWISTDAAPMIRQIRTERAGEVPGETTGPAQALAFSPDGKTLAVAHKDAA
                     DQSVRFWDLTQPGNAPVKLTGSQGGFGSVVFTPDGKEVFAAEPFDPGGLYTFAMDDPP
                     RLSTVQWPRAVIHALAVRTDGQQMAAATAEGVFLWDLSAALPREVQVPGSGSQSYNGV
                     AYSPDGRTLAASASEGVWLFDLNQPYATPTLLRHLGARAPAFSPDGDWLAAIGAEGNT
                     VRLWDRRTTPPAATVLPGVADDGAALAFSPDSHLLAASGTPGTLRLWNLGRPGTDPTL
                     ISAGAEPVSGLAFSPDGATLAAASLDGAVRLWNVADLRQSASAAVQPTILRSPDRGAI
                     PTTATLTWNWSRFN"
     gene            complement(6957..>9128)
                     /locus_tag="VF276_09185"
     CDS             complement(6957..>9128)
                     /locus_tag="VF276_09185"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HEX6607072.1"
                     /translation="GAGAIYFFRGWRSLLWLTVAGGWAVFLLGWQGTVGTGQVATADR
                     WALQGAALFGLLAFWAVPVLRADYVARHPERESRPLFAGNPDLARLAADQVYLLVIGV
                     PLITLAFSRFIWGSQVTQPVWGWWTLAGTLVYAAVAWALRRRLLNLAYTHGMMAILLG
                     TLSLVQILEGNALLFALAVEGTALLVVARRLADRGTAVYGHVVWAGAVTWLAINLAVD
                     LGTWLFFGSRYAVREALSNTALTNLAVIGLLAAASFALGATNLRALYRNIVHFAFVVW
                     LGLQLAGLPYAVGWTMLAWAVYAVLLNVAYKRLPARFTIVETTLPAHAVLAAGLLFFG
                     IDMSSGQAGSLAVFNVKTLLDLAFLAILGGMSFVVRPAPWATAYRLFLHLGVIGLLAR
                     ELAVLPHGVGYIMLAWAVYAAGLNVLHYRWPVRFPDDDTTNPAHLIFLLAAIWFSMDI
                     YTGHTGSLAVFNVQTLIDAIGVAIVAGMSFFARPARLVPAYRIAAHAGVLALLGRELG
                     VLPDGSGYVLLAWAAYLTFAQYLAWRGKDLITAGGTHAGFLLVAAGLAQRLWGDAAGP
                     LPLVNLAALIDLAVIALILGTSWLAQPRVAAWAYRLVAHVALLYWFWRELVTLENGQA
                     LVTIAWGVYALAVVVGGLWGRRRMPLVYAGLATLFVVLGKLFLIDLAAIDPLWRVLLF
                     LGFGGVFLWLSYFFQNAISGKSRPPGARGFGRLHWPGRPAH"
BASE COUNT         1579 a         3220 c         3062 g         1267 t
ORIGIN      
        1 atcatggcgc cggagatgat cacaagcggc aaatcgggtg gaggaggata gtgggccgat
       61 ggagacggaa tcaccgcacg atcaagactg gttctgggag gggaacatcc aggcccgcgt
      121 gctggactac atgcagaacg aggagggctt caccatcctc agtccgggcg aggccatgcc
      181 cgccgagcaa ggcatcgaga tcgtggccga gcgcgcggcg ggtgagcgca cagtccaccg
      241 gctggtcagc gtgcgcggct ggcccagcgc gatccaccgc cgcggtacca tggccgggcg
      301 gccccgctcg acccggcccg aggtcatcgc ccgcagttgg gtagcccagg cggtgctcga
      361 cctggcgctg gggcgcaacg gcgacccgga catcgacctg tgcctggcgt tgcccgccgt
      421 cgccagctac atccgctaca ttcagcgctt gcgctggttc ctggccgtgg ggcgggtgac
      481 ggtgtacctg gtgtcgcagg agggccgcgt ggcggtcacg ccgccggggg cgccgccggt
      541 cagccatttc gccccatccc cgccgccggc cataccggga cagcggcgca agctggggct
      601 gccgggtgcg tcacgtatgc aactgccgct gctgcacgcc ctgatcagcg cgggcggcgc
      661 cgcggggcgg gccgagttga tcccgctggt ggcccgctgg ttcccggagg tgccgcagcc
      721 cccgcccgcc gagttcggcc agcgcctctc ggtggcccag cgggggctgc aagtcgacgg
      781 cctgaccgag ctggacgggc gcggcgtgtg gcgcatcacc gaggcggggc ggaccctcta
      841 cgccgcccag tgggaggact ggcgcgctaa acaggagcgc caggccgcgc ccgaccgcta
      901 acccgcgggc gcttcgctcg cgcgagccgg gccgagggat cgcttaccga gcaaccaggg
      961 cagccaggcg atcatcagcg cggcggccac gaacccggcc caggcgccga agccgaaggt
     1021 ggcgccggga ccaaacgtgc tccagagcca gccgcccagc acattggccg gcagcgccgc
     1081 gaacccggtc acgccgttga accagcccat cgccgtgccg cgctgcgcct tggggatcac
     1141 atcggcgatc agcgccttgc tgaccccgtc ggtggcggcg gcgtagatgc cgtagacggc
     1201 aaatagcgcc cagacatgcc agctctggtt cgccacggtc caccctgcgt agaccagggc
     1261 aaaggacagg tagccggcga tcaggacggg cgcgcggccc cagcggtcgc tcaacacgcc
     1321 gagcggcgtc gccagcacag cgtagatgat gttgtagccg aaatagacga gcggcaccgc
     1381 ctccagggcg ctctccaatc cctcagcccg caggaacagg aacgcatccg acgagttacc
     1441 cagggcgaaa atcgtggaga tgaaggtgaa catccagaag cgcgggccca gggcgcgcac
     1501 ctgctgctgc cagggcactt tcggctgatc ggcggccttg gccggcgtgg ccgggcgctc
     1561 gcgccgctgt tcccgcagga agaccagcgc cagcacgccc agcacgccgg gcaccgccgt
     1621 ccaggcgaac acctgccgca gatcctggtt gttgagcgcc aggatgccca ccgtcagcag
     1681 cgggccgatg gccgcgccga gcgtgtccat cgagcggtgg aagccgaacg ccttgccgcg
     1741 attctgcgcg tcggtcgagt cggccagcag cgcatcgcgc ggactaccgc gcacgccctt
     1801 gcccacgcgg tccagaaaga taaagcccag cgcctgcggc cacgtcatcg ccagcgcgag
     1861 gaacggcttg atgccgttgg ataggccata gccgaccgtg atcagcggtt tgcgcgaatt
     1921 cacccggtcg gacatgcgcc cggagataat cttgaggatg ctgacggtgc tttcggccag
     1981 gccctcgatc agcccggcca ggatcagcgg ggtgttcagc acgccgacca ggaacaggat
     2041 gcgcaccggg acgatcatct cgctgctgat gtcggtgaag aggctgacga tgccgaggac
     2101 gaagacgttg cgcgttagcc cggcgaaagg cccgcgccgg gccggcggcg gggcagggga
     2161 tgggtccatg ctaactccac tgtgtaaaga cgacaggccg gaataccagc cgcattatac
     2221 ggcaacccgc cgcaaatctc caaacgcggg gtagcgcgga ggctcaagcc tccggctacc
     2281 cctacgaagg ccgcctgcgc ggcctcaaag ttacccaccg tatttgacaa cggcccggtg
     2341 cgtttgctat agtgacctgc atcagaccca tactttcact cgcgaggaat gcctgcatgg
     2401 cctcgacggc ccgtaccacg tccgataccc ccgcccgcga tactacgctt gcccctccgg
     2461 cccgccgggt gggccggatt ctcagttggg tgttcctgat tgcgctggcg gcgctgctgg
     2521 cgatcagtaa cccgattctt gtgaacctac aaccccgctt cgatctcgcc ctgctgatcc
     2581 cggcagccgg ggtcatcctg gctgcgctgg gcgcgcggcg tggctggccg ctgctcatct
     2641 ggccgggagc cgccgtgctc tggctgggca tcgtagcctg catcaccacg cagagcagcg
     2701 cgatcggcac ggcgcccgcg ctcgtgtggg gtgagcgcgg gatcgtgctg gtgggggcgg
     2761 gtctgatctg gttcctgctg ctggggctgc ccggctgggt gcagcgcgcc gcgctggcgg
     2821 cggccctgcc cacgctggtc atctttgccg tgctctggat cacggcgccg accgtggtgc
     2881 gcaccgcggg cttctactgg ctggccgccg acgcgcgggg ccgcctctac gccaccgaca
     2941 tcgacaacgg cagcatctgg gtgttcgact ggaacggcaa cgtgagcggg cagatctggc
     3001 cgcgccgcgc cgcgcccggc agccccggcc ccggcatcca gcccgcgggc atgggcagcg
     3061 agtttaccca gcagatcgcg cgcaccacgc ccaaggacct gccgctggac cgcaccttcc
     3121 acacctgcgg catggccgtc gatccggaaa actacctcta cgtgctcgac accgggctgt
     3181 cacgtatcca gcaattcaac ccggatggcc acctgcgcgc cacctggccc ctgccgcaga
     3241 cctatatcag cgcgccgggc tgcctggccg ccgacgcgac ccgtctctac atcgccgata
     3301 accgcgggat catcctcgtc tacgaccatg ccggcgtcca gcagacggag tggaagctca
     3361 gcgggccgtc gcgcgatctc gcggtcggct gggatgggcg attatatgtg ttgcatgacg
     3421 cgagcgtgga agtgctggag atcgccgggg gccggcacct gctgcagtgg gacttgccgc
     3481 ccggcatcgg caccgtgccc tacgacatga tcctggcccg ccgcaacggc gagatcctga
     3541 ttaacaacaa cctcaccgcc caggtgctgc gctatcggcc cagcggcgaa gcgttgccgg
     3601 ccctgggcaa gccgggcacc gagccgggcc agttcggcgt gcccgccggc ctcaccgagg
     3661 acgcggagag ccggctctac atcagcgatg ccgcctaccg cgtcatccag cggttcagcc
     3721 ccagctatca ggtcgaagcg gtctggtcgg tgccggaaga agaaaaggac gaagccgaac
     3781 gacggcgcta gcccaccccg cccggcgagt cggccgggcg aggcggcgga agtcgcgcgg
     3841 gccggtgggt tagcggatcc accgcctcac cactcgcgca cccagggata tgacagcgcg
     3901 tagtctgtaa agcctatggc ggcgtagagc gcgtgcgcgg acggggaata cgagccgacc
     3961 gtcgcgacgg tcgcgcccag gtgctgcgcc cggcgcagcc cctcactcat cacggcgcgg
     4021 gccaggcccc ggcgctggtg ttcgggcgcg gtgccgaccg gctcaaaggc cgccgcgcgc
     4081 gtcacgtcgt cgaaccagac cgtgcagaag gcagcaatct ccccgccagg ggccaccgct
     4141 accaggtcga gatcgcgccg gtacagcggc gcccgctgca cgttctcata ccattcccag
     4201 ccccgatagc ggcccgccgg ctcatccggg tggaaagccc gccacgaagc ccaactgcgc
     4261 gccggtagtt cctcgcggtc gcccagtgcg cgcaccgtat agccggcgcc cggcgcggtc
     4321 tgtagaatcg gtaagtcgag cgagcggcgg cgctggtact cgggcccgga gtcctggcga
     4381 taaccccggc tgcgcagtac ctgctgccgc aagttatcat gttcgttcac ccacaccgcc
     4441 aaccggcggc gcccatcgcg gccggtgtcc gccagttgcg cctccgccct ggcgagcatc
     4501 gcggcttcca gcgcgggtgt gcggagcacc gggtctacct ctagaaacgc ctcacccttg
     4561 ccttcgggat gcaccactgc ggccagccgc ccgtccgccg tttcccagag aaagacggca
     4621 tcgggcagcc agggatgctc gatgttctcc caggcatgcc agcggcaata atcgaagcgg
     4681 tagaccgccc agttgagatc ccaggcccgg ttgccgggag ccacggcgcg caaaaactgc
     4741 cggatccgcc agtagtcgcc gtcgtcatgg taccggcgca tcgtgagttc catcggcgta
     4801 ttacttgctc cctcctggtt gctgcgtgat agcatgccgt ccgtgggcct tatttgcagc
     4861 gggcgcccgg cctgttcggg cgcccgccgg tttacttgcg gggcgggcca ctgcgcccgc
     4921 gcagccgggc cccggcctcc agcagcacca gcgcccaggc caccagcgcg gccagcggcc
     4981 agatcagcga ctctccggtg accggcatgc cgacggacgc cggggtaggg ctgggagtca
     5041 aacttggaac cggcggtgcg tcaccgccgg ttttagcggg agcacgctca gtcatctcga
     5101 cgggacccat cgtctgcgcc aggccaaaat cctaattgaa ccggctccag ttccaggtca
     5161 gtgtagcggt tgtggggatc gcgccgcgat ccggggaccg caggatcgtc ggttgaacgg
     5221 ccgccgaagc gctttggcgc agatcggcca cattccacaa gcgtaccgcg ccatccagcg
     5281 aggccgccgc cagcgtcgcc ccgtcggggc tgaaggccag cccgctcacg ggctcggcgc
     5341 ccgcggagat cagtgtggga tccgtccctg gccggcccag gttccacagc cgcagcgtgc
     5401 cgggcgtgcc gctcgccgcc agcaagtggc tatcagggct gaaggccagc gccgcgccgt
     5461 cgtccgccac gcccggcagg accgtggcgg cgggcggggt cgtccgccgg tcccagaggc
     5521 gcacagtgtt cccttcggcg ccgatggcgg ccagccagtc gccatcggga ctgaaagccg
     5581 gagcgcgcgc gccgaggtgc cgcagcaggg tgggcgtggc gtacggctga ttgaggtcga
     5641 acagccagac accctcgcta gcactcgcgg ccagagtgcg accatccggg ctgtacgcca
     5701 ccccgttgta gctctggctt cccgatcccg gcacctgtac ttcgcgtggt agcgctgccg
     5761 acagatccca gaggaagact ccctcggcgg ttgctgccgc catttgctga ccgtcggtcc
     5821 ggacggccag ggcgtgaatg acagcccgcg gccactgcac cgtgctcagg cgcggcggat
     5881 catccatcgc aaacgtgtat agtccaccgg gatcgaatgg ctcggcagca aagacctcct
     5941 tcccgtccgg cgtgaacacg actgaaccga acccgccctg ggatccggtg agcttcaccg
     6001 gcgcgttgcc cggctgggtc aggtcccaga accggacact ctggtccgcg gcatctttgt
     6061 gcgcgacggc cagagttttg ccgtcgggac taaaggcgag cgcttgcgcc gggccggtgg
     6121 tctcccccgg tacttcccca gcccgctcgg tccggatctg ccggatcatg ggggcagcat
     6181 cggtggagat ccacaactcc accggcgtac ccttggggaa accggcggct tcgctgaact
     6241 caccgacgag atgccgggtc ggcaccccat cgacagcctg ggtgcgcggg ctaccgtcgg
     6301 caatgaccgg attcgccaat cccctactga tgatgctgga tccggctgag gttgcgcccg
     6361 gctgccagca gtgaccgcgc cagacgcata ccacgccgac catcagataa gcagcaggag
     6421 tatcggtttc cggccggctc caggtcttcc cgccgtcgta gctctcgcgc cactcggcgt
     6481 ctgtgaagcg cacctcggtg gcaggcgcga tatactggcc gatagacaac gtcgactggg
     6541 tctcgtcccg cgccactccc ggcgcatagc gcatcgtaac ccggctcccg ttcgcgttgc
     6601 cctgtaggtt agcggcgatg gttaagcttt cgcgctccgc atcaccctgc gcgtagcttt
     6661 caccccggaa ctcgaaatgg tagctcgcca gcccctgcat attggagacg gcctgggcca
     6721 ccagttgctc attggtcaag gtgtcgttcg ggccggattg ggccgccgca ggcgcgggca
     6781 gggtaccggc caggctcacg gcggccagca gggcggccag gatgaggaac cgcgcccggc
     6841 gggctggcga ggcgcgacgt actgtcgtat ccattgataa ctccttgcgg gaggcccgcc
     6901 gcccgactgg gtcgggcggg ccccgaacca tccgagcggc gagcgcctca gtcccgctag
     6961 tgcgcggggc ggcccggcca atgcaggcgc ccaaagcccc gcgcaccggg cgggcgcgac
     7021 ttcccgctga tcgcgttctg gaagaagtaa ctcaaccaga ggaacacccc gccgaagccg
     7081 aggaagagca gcacgcgcca cagcgggtcg atggccgcga gatcaatcag gaacagtttg
     7141 ccgaggacca caaagagcgt cgcaagaccg gcataaacca ggggcatacg gcggcgcccc
     7201 cacagcccgc cgaccaccac ggccagggcg taaacgcccc aggcgatcgt caccagcgct
     7261 tgcccgttct ccagcgtcac cagttcgcgc cagaaccagt acagcagcgc cacgtgcgcc
     7321 acgaggcggt aggcccaggc cgccacccgc ggctgcgcca gccacgaggt gccgaggatc
     7381 agcgcgatga cggccaggtc gatcagggcg gcgaggttca ccaggggcag cgggcccgct
     7441 gcatcgcccc acaggcgctg cgccagcccc gcggcaacaa gcaggaaccc cgcatgggtc
     7501 ccaccggcgg tgatcagatc cttgccgcgc caggccagat actgcgcgaa ggtgaggtag
     7561 gccgcccagg ccagcaagac atagccggag ccgtccggca acacccccag ttcgcgcccc
     7621 agcagcgcca gcaccccggc atgcgccgcg atgcggtacg ccggcacgag ccgggccggg
     7681 cgcgcgaaga aggacatccc agccacgatc gccaccccga tggcgtcgat cagcgtctgg
     7741 acgttgaaca ccgccaagct acccgtgtgg cccgtgtaga tgtccatgct gaaccagatg
     7801 gcagccagca ggaagatcag gtgggcaggg ttggtggtat cgtcatcagg gaaccgcacc
     7861 ggccagcggt agtgcagcac gttcagcccc gccgcgtaga ccgcccaggc cagcatgatg
     7921 taacccacgc catgcggcag taccgctagt tcgcgcgcta gcaacccaat gacgcccagg
     7981 tgcaggaaca gccggtaggc cgtggcccac ggcgccgggc gcaccacgaa ggacattccg
     8041 ccgaggatgg cgaggaaggc gaggtcgagc agcgtcttga cgttgaacac cgccaagcta
     8101 cccgcctgcc cggagctcat atcgatcccg aagaacagca acccggcagc caggaccgca
     8161 tgcgccggaa gcgtcgtctc cacgattgtg aagcgtgccg gcagacgctt gtaggcgaca
     8221 ttcaacagca cggcgtagac cgcccaggcc agcatcgtcc agccgaccgc gtagggcagc
     8281 ccggccagtt gcaggccgag ccagaccacg aaggcgaaat gcactatgtt gcggtacagc
     8341 gcgcgtaggt tggtcgcgcc gagggcgaag gaagccgccg cgagcagccc gatgacggcc
     8401 aggttggtaa gggcagtgtt gctaagcgcc tcccgcaccg catatcggga gccgaagaac
     8461 agccaggttc cgagatcgac agccaggttg atagccagcc aggtcacggc cccggcccag
     8521 accacgtgcc cgtagactgc ggtgccccgg tcggccaggc ggcgcgcgac aacgagtaac
     8581 gccgtcccct cgacggcgag ggcgaaaagc agggcattcc cttcgagaat ctgcaccagg
     8641 ctcagggtgc ccagcaagat cgccatcatg ccatgcgtat aggccaggtt cagcaggcgg
     8701 cggcgcaagg cccaggccac cgcagcataa accagcgtcc cggccagggt ccaccaaccc
     8761 cataccggct gcgtcacctg gcttccccag atgaagcgcg agaaggcgag cgtgatcagc
     8821 gggacgccga taacgagcag ataaacctga tccgcggcga gccgcgccag atcgggattg
     8881 ccggcaaaca gcggcctcga ctcgcgctcc ggatgccggg cgacatagtc ggcgcgcagc
     8941 acgggcaccg cccagaaggc cagcagcccg aacagcgccg cgccctgcag cgcccagcga
     9001 tcggcagtcg ccacctgacc ggtccccacc gtgccctgcc atcccagcag gaacacagcc
     9061 catccaccgg cgacggtaag ccagagcagc gagcgccacc cgcggaagaa gtaaatcgcc
     9121 ccggcccc
//
LOCUS       DASUYC010000559         5059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1826266, whole genome shotgun sequence.
ACCESSION   DASUYC010000559 DASUYC010000000
VERSION     DASUYC010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1826266"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1378)
                     /locus_tag="VF276_09190"
                     /pseudo
     CDS             complement(1..1378)
                     /locus_tag="VF276_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854348.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
     gene            complement(3054..4076)
                     /locus_tag="VF276_09195"
     CDS             complement(3054..4076)
                     /locus_tag="VF276_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607073.1"
                     /translation="MPLLATAERVVKCPTGHCPGWITVDDERPQTPPCRYCRVTVNTL
                     SFLLESASETGAAPQMPAAVHETFNGLLLKLAGMTGGNVYLRPIDRTIVSLVPSTRGV
                     IIEYNPMIARHAGPAAVVALILHHLLHIEQHPRHEKPLGMVLRAGARDKEAMQPVANY
                     VLTLVDHAWITPRIDAIDPALGEAARTWGLDVAALLTGNETFFPPYMAERNRRWISDQ
                     FAGAADSAALGEALVTRLASLSARFFAGQRDDLRRNLLAVQLADLGVRDPARQAPYAA
                     ALEAAGVPNVAVAAGTAARLVLEIDEVRADSPTYDPIAYRRGLEAALKALGLQTVFEV
                     QTLKSS"
     gene            complement(4348..>5059)
                     /locus_tag="VF276_09200"
     CDS             complement(4348..>5059)
                     /locus_tag="VF276_09200"
                     /inference="COORDINATES: protein motif:HMM:NF014886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX6607074.1"
                     /translation="VPLIQKRIIREVNRRGKLVITATQMLDSMIRNPRPTRAEATDVA
                     NAVLDGSDAIMLSGETATGKYPLAAVQIMARIALEAERVFDYEGWTAHVAALAGPPAP
                     GTPPRPDALVMTEAVCQAADHMAEELGACAIVALTRTGTSARMVAKYRPRGLLIALTD
                     QPATRHALAVTWGVQALLTAPFGDTLTTLAAAERRVLEAGLVRPGDILIFVGSLPQME
                     PGRTTMLQVQVAGSLSAG"
BASE COUNT          732 a         1664 c         1768 g          895 t
ORIGIN      
        1 tgtcggtgcc gcggcccgcc atgttggtgg cgatggtcac ggcgccccgc tgaccggcct
       61 gggccaccag gctcgcctcg cgctcgtgct gcttggcgtt gagcacgctg tggtcgatgc
      121 cctcggcgtt gagcatggcc gctaattctt cggacttctc gatggaaacc gtaccgacca
      181 ggatcgggcg gccttgctcg tggatttcct tgatttcctc gaccacggcg cggaacttgg
      241 ccgtttcggt cttgtagacc cggtcgggga agtcctggcg caccatgggc aggcgcgtcg
      301 ggatcgcggt ggcttcgagc ttgtagatct tgtagaactc ctccgcctcg gtcagcgccg
      361 taccggtcat gccggccagc ttctggtaca tgcgggcgta gttctggatg gtgacggtgg
      421 cgtaggtctg gctctcgccg cgtacccgga tgccttcctt ggcctcgatg gcctcgtgca
      481 gcccctcgga gtagcggcgg ccttccatcg ggcgcccggt gaactcgtcc acgatgacca
      541 cttcgacggg ctgcttggga ttcagctttt cgcccggcga gatgatcttg ccgtcctgca
      601 tcagcacata gtcgcggtcg cggcggaaca gagcgtgcgc cttgagcgcg ttgtccaggc
      661 cgtagaccag catgaagttg tcggggtggt acaggttggt gatgtggaag aactgctcgg
      721 cgcgctccac gccttcgtcg gtgaacacgg cgctgcgcga cttctcgtcc acctcgtagt
      781 cgcgcttggc gaccagggtg cgggcgaaag ccgccgcctt ctcatgcaca tcgctggtgt
      841 tctgcgacgg gccgctgatg atcagcgggg tgcgggcttc gtcgatgagg atgttgtcca
      901 cctcgtcgac aacggcgaag tgatggccgc gctggaccac ctgctccggc cccggcgcgg
      961 tgttgtcgcg caggtagtcg aagccgaact cggagttggt gccgtaggta atatcggcct
     1021 tgtactcgcc cacccgctca tacgcggtca tgtcgttgag gatcacaccg acggtcaggc
     1081 cgagcagggc atagaccggc cccatccagt cgctgtcgcg cttcgccagg tactcgttga
     1141 ccgtgatcag gtggacgccg cgggcggtga gcgcgttcag cgagagcggc agcgtggcga
     1201 cgagcgtctt gccttcgccg gtcttcatct cggcgatctt gccctcgtgc agcgtgatcc
     1261 cgcccatgaa ctgcacgtcg gcggggcgca ggccgatggc ccggcgcgcc gcgtcccaga
     1321 tgcaagcaaa cacgacgggc agaacttcgt tgagcagatc ctgttcctgc ttccggtcgg
     1381 cctcgctgaa ttcggtctgc agggcgcgca agcggtcggc ctgctcgcga tccccggcgg
     1441 tacgcagttc ggcgtggaag cggcggaaga agtcttcgat cagattgtcg cgcgccgtca
     1501 tcacttgctg gagatgcagc ggcttgacgg ccagcggcgg gttcagcccg gcctcctcct
     1561 cggcgtcgag ttcggccagg agggcgtcct ggcgctcctg caaggcggcc agcgcctcca
     1621 ggaacggacc cacggcctcg gggaagtcgg cttccaggcg atcctggtag gtctgccaga
     1681 ggtcggtgcg ggcgcgcagc agttggccgc gcaccacggc catgttgcct tgcaggcggt
     1741 cggtgcgcgc cgcgaagcgg gcctgtagct cggccagcgt ggcctgcggg gtctcgccgg
     1801 cttcagcgcg ccccaacgcc acggcggtca gcgccagcag ggtctcggcg gcggcctcct
     1861 gccaggtcag caggtcggcc agatcgtctt tgggcgcgcg caccgtgccc gcggcgatgc
     1921 cctgctgcgg ggtgagcggg acgggcgcag gcgccgactt gcggcggcca aacagcccgc
     1981 cgccgctcga cgtattcgcc acggcggcgg acggcaccgg cccggcggcc gccagcagtt
     2041 cgcccggcag caacagcccc tggcgctgta ggtgcttcat caggtcgttg gcttgtagcg
     2101 cgtccaccag ggcgcgcgtc gccgtgcgca gcttgtcgcg atctccggcg acggcggcct
     2161 gctcggcgcg catcccctcc agggggtgcg gaccgcggtc cacctcggcg gcctgcccgc
     2221 ccgccagcga cgtgtcgccg agcaaggcca gcagcgcggt agcgtggcgt aacccgtcga
     2281 gcagatcttg caggcggcgg aagtagccgg cgatctccgg ctccagggcc gtggaggtct
     2341 gctccaccat cttgccgatt tcgtccggcg tggcccgccc gctcttgcga tcctccaggt
     2401 tctgcaggcg gctcgtcacc gcctccatgg ccgggcgatc cgcgctgaac gcgcgcaact
     2461 gggtgaggat gcggtcgctc aggtcggcga tgttggcctc cacctcctgg cggtcgcccg
     2521 tacgccgggc cagttcggcc atgagctggg caagctggcg gctgtcggcg agcagcacgt
     2581 tcatgttgcg cgtggtcgcc tcgcccacct cgatcacccg gcgcagttcc ggggcctggg
     2641 cggtcaggct ctggacggtg gtcgtggcct cggccagcga ctcgcgcagc aagtcgggca
     2701 gcacccgcct cgcgttgctc tcgcgatgga tgagcgtgtg caggtcggtg cgtagcgccg
     2761 ccagcgccgc cagttggtcc gtcagcaact cctcgatctc gtcgttgacg gtggcgacaa
     2821 tcttgcgcag cctggtaagt tctttgtcct gggagtcgac aaagtttccc agccacttaa
     2881 acataagaga tcctctttca agttagccgt cagctatcag ctatcagccg tcagctatca
     2941 ggtaataata agcgatttaa gtaaaaatag gcagcccaca atttatgagc cggagataaa
     3001 gctaacagcg ggccatcaaa tcctgacggc tgacggctga cggctgacgg ctatcagcta
     3061 cttttcagcg tttgcacttc aaagactgtt tgcaggccca gggctttcag cgcggcctcc
     3121 aggccccgcc ggtaggcaat cgggtcgtag gtcgggctgt cggcccgcac ctcgtcgatc
     3181 tccaggacca ggcgggcggc ggtgccggcg gcgacggcca cgttgggcac gccggccgct
     3241 tcgagcgccg ccgcgtaggg ggcctggcgc gcggggtcgc gcacgccgag atcggccagt
     3301 tgcacggcca gcaggttgcg gcgcaggtcg tcgcgctggc ccgcgaagaa gcgggcgctg
     3361 aggctcgcca ggcgcgtcac cagggcctcg cccagggcgg cgctatcggc ggccccggcg
     3421 aactgatcgc tgatccagcg gcggttgcgc tcggccatat agggcgggaa gaaggtctcg
     3481 ttgccggtca gcagggccgc gacatccagc ccccaggtgc gcgccgcctc gcccagcgca
     3541 gggtcgatag cgtcgatgcg cggcgtgatc caggcatggt cgaccagggt gagcacgtaa
     3601 ttggcgacgg gttgcatcgc ttctttgtcg cgggcgcccg cgcgcagcac catgcccagc
     3661 ggcttctcat ggcgcgggtg ctgctcgatg tgcagcagat ggtgcaaaat cagggcgacc
     3721 acggcggcgg gcccggcgtg ccgggcgatc atcgggttgt actcgatgat cacgccgcgc
     3781 gtgctgggca ccaggctgac gatggtgcgg tcgatggggc gcaaatagac gttgccgccg
     3841 gtcatcccgg ccagtttcag cagcaagccg ttgaacgtct catgcacggc ggcgggcatc
     3901 tgcggcgccg cgccggtctc ggatgcactc tccagcaaaa agctcagcgt gttgaccgtg
     3961 acccggcaat agcgacacgg cggcgtctgc ggccgctcgt catcgacggt gatccagccg
     4021 gggcagtgcc ctgtggggca tttaaccacc cgctcggcgg tggcaagaag tggcatggtg
     4081 gtgaatactc ctcaattccc agtccgcaaa tggcggagag tacgcaaggc gagatagact
     4141 atctacaatt gtacccgaaa gggcgcggct gtgccactgg gttctctttt taaagtacca
     4201 cccagaatac gtatgagagg gccgcctggt tgcccagtct ctaccctgtc attctgagcg
     4261 cagcgaagaa tctcggtctc cttcgggcca acgacgagac tcttcgctcc gctcagagtg
     4321 acatggggcc gggccgcccg gttgccctca gcccgcgctg aggctgcccg cgacctggac
     4381 ctgcagcatc gtcgtgcgac ccggctccat ctggggcagg ctgcccacga agatgaggat
     4441 atcgccgggg cggaccagcc cggcctccag cacccggcgc tcggcagcgg ccagcgtggt
     4501 cagggtgtca ccgaacggcg ccgtcagcag cgcctggacg ccccaggtca cggccagggc
     4561 gtgccgggtg gcgggctggt cggtcagcgc gatcagcagg ccgcggggcc ggtatttggc
     4621 gaccatgcgc gcgctggtgc ccgtgcgcgt caaggccaca atggcgcacg cgcccagttc
     4681 ttcggccatg tggtcggcag cctggcagac ggcctcggtc atcacgagcg cgtcgggccg
     4741 gggcggcgtg ccgggggcgg gcggcccggc cagcgccgcg acatgcgcgg tccagccctc
     4801 gtagtcgaac acgcgctcgg cctccagggc gatgcgggcc atgatctgca cggcggccag
     4861 cgggtacttg ccggtggccg tctcgcccga cagcatgatc gcgtcgctgc cgtccagcac
     4921 ggcgttcgcc acgtcggtgg cctcggctcg cgtcgggcgc gggttgcgga tcatcgagtc
     4981 gagcatctgg gtcgcggtga tcaccagctt cccgcgccgg ttcacctcgc ggatgatccg
     5041 cttctggatg agcggcacg
//
LOCUS       DASUYC010000560         4971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_955972,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000560 DASUYC010000000
VERSION     DASUYC010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4971
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955972"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VF276_09205"
     CDS             <1..398
                     /locus_tag="VF276_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEX6607075.1"
                     /translation="AVAGTLDPTLTTVLSPFDPLVWDRNRAHELFDFDYKIECYTPAA
                     KRRYGYFTLPILHRGRLIGRLDAKAHRAQGMFEIKALHLEPGVTVDESLVDDLAAALR
                     RLAGWHGTADLVIRRSNPPEVATLLAAAV"
     gene            complement(441..1256)
                     /gene="nth"
                     /locus_tag="VF276_09210"
     CDS             complement(441..1256)
                     /gene="nth"
                     /locus_tag="VF276_09210"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX6607076.1"
                     /translation="MPPIDTKQREKWPAPSPGPDAATTAAQVDLAREEAATDPLRAKA
                     RAVLALLNAAYGEPAWPVLDPLATLIEVILSHRTADPQTWAAYQALRARWPTWEALRD
                     APVAEIEEAIHGTTWPEQKAPRIKRVLQTITERHGTLDIDFLKTLDLPAAQEWLTDLE
                     GVGPKSAACVLLFACRRPVLPVETHLHRVSQRLGLIGPKVTAEQAHTILQALLPEPEN
                     PRVVLEFHRNMLLHGQRVCVWRDPRCAVCVVRDWCDYFAAHPEKQAEAARRAR"
     gene            complement(1648..2505)
                     /locus_tag="VF276_09215"
     CDS             complement(1648..2505)
                     /locus_tag="VF276_09215"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HEX6607077.1"
                     /translation="MTGRNRSIIVLGVVFLVLAGISYWLTANPATPPTPTPEPTYPVW
                     DYGAATVQGVTVQSPTSTVTLQVKDGKWEITAPVQQPADDLQISQATDPFKRAEGRAK
                     IGDNVTDLAQYGLDSPALTVTLVLSGATVPKAELLVGKPSVDGSAYYARPQGSNAVYL
                     LNNTLIEPFKSWLTTPPIAQPTATPIPATIIPAPSPPTSTGTLSGTESLPLTPGTTPE
                     GPVAPATGTPEARPATANTPSAAGTEPPVGASPVSSPPASGTDASPVPSGSGGGAGGT
                     PGTVSTPTP"
     gene            complement(2509..4170)
                     /locus_tag="VF276_09220"
     CDS             complement(2509..4170)
                     /locus_tag="VF276_09220"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HEX6607078.1"
                     /translation="MSSNPQSVPASDLSSNRALIANAAGWFGLIMLIGYLLYWGVTGN
                     NFDLPAKVLLILGLLAIGTFGLMNPQGIIDIVSGQGTRTILSTVLLLALGFGIIVAIN
                     IIYGEIVKRAPSPILRTDLTEGQQNSLSPQSIRAVQELTGTVTAYGFFGTTGTEISQQ
                     RDAENLLKEYQKYSNKLKVEFVNPDVALAQANRYGLTRYDVVVFDNGVRHETADSTSE
                     ENFTGALLRLRSSTQKTVAILNVPSVLDFAGSSQQALAATLARRGLDTENYTVLPTYN
                     LVVSPTISVTDVNVMIVPPSPENQPLPDNAVRALMDYLDHGGHVLLIGDPLAAPLPAA
                     LLQKYGLSEARGIIVDQDRNSLWGNSPTQLLVLTYPSSTITRDMNNVPTIYGAAEPIV
                     VATTTITGFTTTPYIQSSNTAQYAVIKADASGNQSQLALEPNGPQAPMNIAVAVEQTV
                     EDTGNFTSTETTKPQVTRLAVIGDYDMLSDQLISQAPGNLDMFNNTVNWLSQSEERIS
                     VRPKDTTPRQLTMTEQQQSLIAWSTIVFLPVLILLGGGLVWWRRR"
     gene            complement(4167..4871)
                     /locus_tag="VF276_09225"
     CDS             complement(4167..4871)
                     /locus_tag="VF276_09225"
                     /inference="COORDINATES: protein motif:HMM:NF024741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6607079.1"
                     /translation="MWTIAVKELRSYFVSPIGYVALAFWLFGVGLFFALGVLQSQQAT
                     MQDWFSTVEVLLLFIGPALTMRLLAEEQGSGTWELLMTSPVREWEVVLGKFLGATLFW
                     LGMIIVTFFYPLILRLLGNPDMGPIFSGYLGLFLMGAAFLAIGVFTSSLSGNQIIAYI
                     VAFIALLALWIIGFITPVISGDLGNRLAALSVTTHFDDFGKGVIDFSNVLFYLSLMAA
                     ALFATVQVMEARRARQ"
BASE COUNT          771 a         1577 c         1761 g          862 t
ORIGIN      
        1 atgccgtcgc cggaactctg gaccccacgc tgactacggt gctctcgcct ttcgatccgc
       61 tggtatggga ccggaatcgc gcacacgaac tgttcgattt cgactataag atcgagtgtt
      121 atacccctgc cgcgaagcgc cggtacggct atttcaccct gcccatcctg caccgcggca
      181 ggctgatcgg ccggttggat gccaaggcgc atcgggcgca ggggatgttc gagatcaaag
      241 cgctgcatct ggaacccggc gtaacggtcg acgaaagctt ggtagacgat ctggctgccg
      301 ctctgcgccg gttggccggc tggcatggaa cggccgatct ggtcatccgc cggtccaatc
      361 cccccgaagt ggcaactctc ctggcagccg ccgtataggg caagttgctg gatcgccggc
      421 gagccgcggc ggacaactgt tcagcgcgct cgccgcgcgg cctcggcctg cttttcgggg
      481 tgggcggcga agtagtcgca ccagtcgcgg accacgcaga cggcgcagcg cgggtcgcgc
      541 cagacgcaga cccgctggcc gtgcagcagc atgttgcggt ggaactccag caccacgcgc
      601 ggattctcgg gctcgggcag caacgcctgc aagatggtgt gcgcctgttc cgccgtgacc
      661 ttcggcccga tcagtcccag gcgctggctg acgcggtgca ggtgggtctc gaccggtagc
      721 accgggcgcc ggcaggcgaa cagcagcacg caagccgccg acttgggacc gacgccttcg
      781 aggtcggtca accactcctg cgcagcgggc aggtccagcg tcttgaggaa gtcgatgtcc
      841 agggtgccgt gccgctcggt gatcgtctgc aacacgcgct tgatgcgtgg cgccttctgc
      901 tcgggccagg tcgtgccgtg aatggcctcc tcgatctcgg ccaccggggc gtcgcgcagc
      961 gcctcccagg tgggccagcg ggcgcgcagg gcctgatagg cggcccaggt ctgcgggtcg
     1021 gcggtgcggt gcgagaggat gacctcgatg agggtcgcca gcggatcgag caccggccag
     1081 gcgggttcgc cataggcggc gttgagcagg gccagcacgg cccgcgcctt ggcccgcagg
     1141 gggtcggtcg ccgcttcctc gcgggccaga tccacctggg cggcggtcgt cgcggcgtcg
     1201 gggccagggc tgggcgcggg ccatttctcg cgctgtttgg tatcaatcgg cggcatcgtc
     1261 gtcttccctc acctacgtcc tggggcggca gggcggacac atcggtccgc ccctacgcaa
     1321 cctcggcatc ccggtacggg gccgggcgga tacgtgggtc cgccccgtca cgggccatgc
     1381 atcccggtac ggcggcaggc gcgatcaatc gcgccgctac gcatggggcc gcctgtctgt
     1441 ggcgttattt cgtagtggcg cgatttatcg cgcctgccgc cacagccacc cctggccgat
     1501 tccgcgtgca agctccacca gccatttaga acgtatcagc gaccacagcc gcgcgcctcc
     1561 cgcccggcaa aagcgaaccg gcccaggcat tgcctggacc ggtcctgatt ggcctattca
     1621 cgtcacctga tagctgaccg ctgacggcta tggtgtcggc gtgctcaccg tgccgggggt
     1681 gccgcccgcg ccgccgcccg atcccgaggg gacggggctg gcgtcggtgc cgctcgccgg
     1741 gggcgaggac accgggctgg cgccgaccgg cggctccgtg ccggcggcgg acggggtgtt
     1801 cgccgtggcg ggcctggcct ccggggtccc ggtggccggg gccaccggtc cctcaggcgt
     1861 cgtgccgggc gtcagcggca gcgactcggt gccgctgagg gtgccggtgc tggtcggcgg
     1921 cgagggcgca gggatgatcg tcgccgggat cggcgtggcc gtcggctgcg cgatgggcgg
     1981 cgtggtcaac cagctcttga acggctcgat caaggtgttg ttgagcagat agaccgcgtt
     2041 gctgccttgc gggcgcgcat agtaagccga gccgtccacg ctgggcttgc cgacgagcag
     2101 ttcggccttg ggcacggtgg cgccgctaag caccagcgtg accgtcagcg cggggctgtc
     2161 cagaccgtac tgggccagat cggtcacgtt gtcgccgatc ttggcgcggc cctcggccct
     2221 cttgaacggg tcggtcgcct ggctgatctg gagatcgtcg gcgggctgtt gcaccggcgc
     2281 ggtgatctcc cacttgccgt ccttgacctg caaggtcacg gtgctggtcg ggctttgcac
     2341 ggtcacaccc tggaccgtgg cggcgccgta gtcccagacc gggtaggtcg gctcaggcgt
     2401 gggcgtgggc ggcgtggccg ggttggcagt cagccagtag ctgatgccgg ccagcaccag
     2461 gaacaccacg ccgagcacaa tgatacttcg attacgtccg gtcatcagtt acctcctccg
     2521 ccaccacacg agaccgccgc ccaacaagat caggaccggc aagaacacga tggtgctcca
     2581 ggcgatcagg ctttgctgct gctcggtcat ggtgagttgc cgcggagtgg tatccttggg
     2641 ccggacagag atgcgctctt ccgactgcga gagccagttg acggtgttgt tgaacatatc
     2701 gaggttgccc ggcgcctgcg aaatcaactg gtcggacagc atatcgtagt cgccgatcac
     2761 cgccaggcgc gtgacctggg gcttggtcgt ttcggtgctg gtgaagttgc cggtgtcttc
     2821 caccgtctgc tccacggcca cagcgatgtt catcggcgcc tgcgggccgt tgggctcgag
     2881 cgccagttgc gactggttgc cggaggcgtc ggccttgatc acggcatact gcgcggtgtt
     2941 gctgctctgg atatacggcg tggtagtgaa gccggtgatc gtcgtggtcg ccacgacaat
     3001 cggctcggcg gcgccgtaga tcgtcggcac gttgttcatg tcgcgggtaa tggtgctgct
     3061 ggggtaggtc agcacgagca actgcgtcgg cgagttgccc cacaggctgt tgcggtcctg
     3121 gtcgacgatg atgccccgcg cctcggacag gccgtacttc tgcaacaggg ccgccggcaa
     3181 cggcgcggcc agcgggtcgc cgatcagcag cacatggccg ccgtggtcga ggtagtccat
     3241 gagcgcgcgc acggcgttat cggggagcgg ctggttctcg ggcgacggcg gtacgatcat
     3301 cacgttcacg tccgtgaccg agatggtcgg gctgacgacg aggttatagg tcggcagcac
     3361 cgtgtagttc tcggtgtcga ggccgcggcg ggccagagtg gcggcgagcg cctgctggct
     3421 gctgcccgcg aagtcgagca ctgagggcac gttcaagatc gccaccgttt tctgcgtgct
     3481 actgcgcagg cgcagcagag cgccggtgaa gttctcctcg ctggtgctgt cggcggtctc
     3541 gtggcgcacg ccgttgtcga agaccaccac gtcgtagcgc gtgagcccgt agcggttggc
     3601 ctgggccagc gccacgtcgg ggttgacaaa ctccaccttg agtttgttcg agtacttctg
     3661 atattccttc agcaggttct cggcatcgcg ctgctggctg atctcggtgc cggtggtgcc
     3721 gaagaagccg taggcggtga cggtgccggt cagttcctgg accgcgcgga tcgactgggg
     3781 cgagagcgag ttttgctggc cctcggtcag gtcggtgcgc aggatggggc tgggcgcgcg
     3841 cttgacgatc tcaccgtaga tgatgttgat cgccacgatg atgccgaagc ccagggccag
     3901 cagcaggaca gtgctcagaa tggtgcgcgt gccctggccg gacacgatgt cgatgatgcc
     3961 ctgcgggttc atcaggccga aggtgccgat cgccagcaag ccgaggatca gcaagacctt
     4021 ggccggcagg tcgaagttat tgccggtcac gccccagtac agcaggtagc cgatcaacat
     4081 gatcaggccg aaccagcccg cggcattggc gatcagggcg cggttggaac tgaggtcgga
     4141 ggccggcacg gattgcgggt tgctgctcat tggcgggctc tccgtgcttc catgacctgg
     4201 acggtggcga acagcgccgc cgccatcagg ctgagataga acaagacatt actgaagtcg
     4261 atcacgccct tgccgaagtc gtcgaagtgg gtggtgaccg agagcgcggc caggcggttg
     4321 cccaggtcgc cggagatgac cggcgtgatg aagccgatga tccagagcgc cagcagggcg
     4381 atgaaggcca cgatgtaggc gatgatctgg ttgccgctga gcgacgaggt gaacaccccg
     4441 atcgccagga aggcggcgcc catcaggaac aggcccaggt agccgctaaa gatcggcccc
     4501 atgtcggggt tgcccagcag gcgcaggatc agcgggtaga agaaggtgac gatgatcatg
     4561 ccgagccaga agagggtcgc gcccaggaac ttgcccagca cgacttccca ctcgcgcacc
     4621 ggcgaggtca tcagcaattc ccaggtgccg ctgccctgct cctcggccag caggcgcatg
     4681 gtcagcgcgg ggccgataaa cagcagcagc acttcgacgg tgctgaacca gtcctgcatg
     4741 gtcgcctgct ggctctgcaa cacgcccagc gcgaagaaca ggcccacgcc gaacagccag
     4801 aaggccaggg ccacatagcc gatgggcgag acgaaatagg agcggagctc cttgaccgcg
     4861 atggtccaca tgtttctcac ttgcggccgc ctttccgctc gcgcgccggc gccggggcgc
     4921 tcggggccgc cgcttcggct tccgccgccg cggcctcggc ttccgcctct g
//
LOCUS       DASUYC010000561         4309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_696682,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000561 DASUYC010000000
VERSION     DASUYC010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696682"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3124)
                     /gene="fdh"
                     /locus_tag="VF276_09230"
     CDS             complement(<1..3124)
                     /gene="fdh"
                     /locus_tag="VF276_09230"
                     /EC_number="1.17.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(2543..2545),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HEX6607080.1"
                     /translation="MTTNGNGDGQGPGSPISLLGRVARNVGVVPDRTTSRRTERVRPR
                     ISGAQVTDSVCPYCAVGCSQLIYHRDGQVINIEGNYASPINQGTLCPKGAATYQLVDN
                     PHRLHTVKYRAPHSGHWEDRPLDWAMERIAQLVKETRDATWEATDAEGHTINRTRAIA
                     HLGGATLDNEENYLIKKLFAGLGMVWIENQARIUHSSTVPGLGTRLGRGGATTFEQDL
                     ANSDCIVVMGSNMAENHPIAFRFALKARERGARLIHVDPRFSRTSALADTYVRIRSGT
                     DIVFLGGLINYMIQNERYFKEYVLAYTNAAVLINDDFKDTEDLDGLFSGFDPEKRQYD
                     TSTWQYKGHEDEGMEMEKRPENAPETAGQVAQGGGQAAKHTTSQTHQNPRMAPTDPTL
                     QDPRCVFQILKRHFSRYTPEMVADLCGCKPEDFLAVAEALAANSGRERTSAFCYAMGW
                     TQHTVGVQMIGCAALLQLLLGNIGRPGGGILALRGHATIQGSTDIPTLYNLLPGYLAQ
                     PSTTSGHYSLKEYLDVQTPHTGWWHHYPKYMISLLKSWWGDAATADNDYMYDYIPKID
                     SDYSLLPMMQAMKDGQIQGLFCMGQNPAVGTQNAVLVRQGLARLKWLVVRDNFEVESA
                     AFWRNSPEVESGQVRPQDIGTEIFLMPTAMVPEKNGSFTNTMRLVQYHDKAVEPPADC
                     RSEAWFVYHLGRRLRELYAGSTDPRDQGLLKLTWDYGSEGATHDPVIDEIVKEVNGWT
                     VADHKQVKSFTDLKDDGSTTCGCWIYCGIYPDEHTNMARNRDGDNWVSLGWGFSWPAN
                     RRILYNRASADPEGRPWSERKRYVWWDAEQKKWTGYDVPDFPATKAPDTPPNPDGTGI
                     DAHSGADPFIIQADGRGWLFVPSGLKDGPLPTHYEAEEAPVENALYKQRTNPVAKVWR
                     RKDNVLADFGDPRYPYVITTYRLTEHHTAGGMTRWLPWLAELQPYGFVELSPALAQEK
                     GISNGDWVVVSTPRGDAEARAMVTDRMQPMKIRRRTVHQIGMPWHFGWQGLATGGIAN
                     NLTSMVADPNV"
     gene            complement(3219..>4309)
                     /locus_tag="VF276_09235"
     CDS             complement(3219..>4309)
                     /locus_tag="VF276_09235"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEX6607081.1"
                     /translation="GVGAIVGGTYLDKYGPRAVATVAGLFYGIGFIGAGLIQSLWGLW
                     LTYGVLSGIGMGLGYIVPLATLIKWFPDKRGLITGLAVAGFGGGAVVTTLIAPGLISG
                     VGVYTTFIILGIVYLIMVAGAAQFYRVAPAGYTPPGWTPTATQASQRAGKDFTLQESL
                     RTWQWYVLWAMLFLNVTAGVMVISQAAPMTQEITGVDATTASSIVILISIFNGAGRLF
                     WAWLSDLITRRWVFLAMFLIQFVLFLIMPAITDFFLLSVFFMIVALCYGGGFGTMPAF
                     AADYFGPKFAGRIYGLMLSAWSAGGVLGPILIAQVKDNTGKYSTALTIVAVVMLVSAV
                     LPFIARPPRDPAEATSERTAPAPGQSRV"
BASE COUNT          641 a         1360 c         1531 g          777 t
ORIGIN      
        1 tgacgttcgg gtcggccacc atggatgtca ggttgttggc gatgccgccg gtcgccaggc
       61 cctgccagcc gaagtgccag ggcataccga tttgatgcac cgtgcggcgg cggatcttca
      121 tcggctgcat ccggtcggtg accatggccc gcgcctcggc gtcgccgcgc ggcgtgctga
      181 ccaccaccca gtcgccgttg ctgatgccct tttcctgggc cagcgccggg ctgagctcca
      241 cgaagccgta cggctggagc tcggccagcc agggcagcca gcgggtcatg ccgcccgccg
      301 tgtggtgctc ggtcagccgg taggtggtga taacgtaggg atagcgcggg tcgccgaagt
      361 cggccagcac gttgtccttg cgccgccaga ccttcgccac cgggttggtg cgttgcttgt
      421 agagggcgtt ttcgaccggc gcctcttcgg cctcgtagtg cgtgggcagc gggccgtcct
      481 tcagcccgct gggcacaaac agccagccgc gcccgtcggc ctggatgatg aacggatcgg
      541 cgccggagtg ggcgtcgatg ccggtgccgt cggggttggg cggcgtgtcg ggcgccttgg
      601 tggccgggaa gtcgggcacg tcgtagccgg tccatttctt ctgctcggcg tcccaccaga
      661 catagcgctt gcgctcggac cagggccgcc cttcggggtc ggcgctggcg cggttgtaga
      721 ggatgcggcg gttggcgggc caggagaagc cccagcccag gctgacccag ttatcgccgt
      781 cccggttgcg cgccatgttc gtgtgctcgt ccgggtagat gccgcagtag atccagcagc
      841 cgcaggttgt gctaccgtcg tccttcaggt cggtgaagct cttgacctgc ttgtggtctg
      901 ccaccgtcca gccgttgact tccttaacga tctcgtcgat caccgggtcg tgggtggcgc
      961 cctcggagcc gtagtcccag gtcagcttga gcaggccctg gtcgcgcggg tcggtgctgc
     1021 ccgcgtagag ttcgcgcagg cgccggccca ggtggtagac gaaccaggct tcggagcggc
     1081 agtcggcggg cggctcgacg gccttgtcgt gatactgcac cagacgcatg gtgttggtga
     1141 acgagccgtt cttttcgggg accatggccg tgggcatcag gaagatctcg gtgccgatgt
     1201 cctgcgggcg cacctggccg ctctcgactt cgggcgagtt gcgccagaag gcggcgctct
     1261 cgacctcaaa gttgtcgcgc accaccagcc acttgagccg ggccagcccc tggcgcacca
     1321 gcacggcgtt ttgcgtgccg acggcggggt tctgccccat gcagaagagg ccctggatct
     1381 ggccgtcctt catcgcctgc atcatcggca gcagcgagta gtcggagtcg atcttgggga
     1441 tgtagtcgta catatagtcg ttgtccgccg tggcggcatc gccccaccat gacttgagca
     1501 ggctgatcat gtacttcggg tagtggtgcc accagccggt gtgcggcgtc tgcacgtcca
     1561 ggtactcttt caggctgtag tgcccggacg tggtggacgg ctgggccagg tagccgggca
     1621 gcaggttgta gagcgtgggg atgtcggtcg agccctggat ggtggcgtgg ccgcgcaggg
     1681 ccaaaatgcc gccgccgggt cgcccgatgt tgccgagcag cagttgcagc agcgccgcgc
     1741 agccgatcat ctgcacgccc acggtgtgct gggtccagcc catggcgtag cagaaggccg
     1801 acgtgcgctc gcggcccgag ttcgcggcca gcgcctcggc cacggccagg aagtcctccg
     1861 gcttgcaccc gcacaggtcg gctaccatct ccggcgtgta gcgcgagaag tggcgcttga
     1921 ggatctggaa gacgcagcgc gggtcctgca gcgtagggtc cgtgggcgcc atgcgcggat
     1981 tctgatgggt ctggctggtg gtgtgtttgg cggcctggcc gccgccctgg gccacctggc
     2041 cggcggtttc gggcgcgttc tcgggccgtt tttccatctc catgccctcg tcttcgtgcc
     2101 ctttgtactg ccaggtgctg gtgtcgtact gccgtttctc cgggtcgaag cccgagaaga
     2161 ggccgtcgag atcttcggtg tccttgaagt cgtcgttgat caggacggcg gcgttggtgt
     2221 aggcgagtac gtactccttg aagtagcgct cgttctggat catgtagttg atcaacccgc
     2281 cgaggaacac gatgtcggta cctgagcgga tgcgcacata ggtatcggcc agcgccgagg
     2341 tgcggctgaa gcgcggatcg acgtggatca ggcgcgcgcc gcgttcgcgg gccttgaggg
     2401 cgaagcggaa ggcgatcggg tgattttcgg ccatgttcga ccccatcacc acgatgcagt
     2461 cgctgttcgc caggtcctgc tcaaaggtgg tcgcgccgcc gcgaccgagg cgggtgccca
     2521 gaccgggcac agtggagctg tgtcagatac gggcctggtt ctcgatccag accatgccca
     2581 gcccggcaaa cagcttctta atcaggtagt tttcttcgtt gtcgagggtc gcgccgccca
     2641 ggtgggcgat agcccgtgtg cggttgatgg tatgcccctc ggcatccgtc gcctcccagg
     2701 tggcgtcgcg cgtctccttg accagttgcg cgatgcgctc catggcccag tcgagcgggc
     2761 ggtcttccca gtggccgctg tgcggcgcgc ggtacttgac cgtgtgcaac cggtgggggt
     2821 tgtccaccaa ctggtaggtc gccgcgccct tggggcagag cgtgccctgg ttgatcgggc
     2881 tggcatagtt gccctcgatg ttgatcacct ggccgtcgcg gtggtagatg agttgcgagc
     2941 agccgaccgc gcagtaggga cagacgctgt cggtcacctg cgcgccgctg atgcgcgggc
     3001 gcacgcgctc ggtccgccgg ctggtggtgc ggtcggggac gacgccgaca ttgcgcgcga
     3061 cgcgccccag gagggagatc gggctgccgg ggccctggcc gtctccgttg ccgttcgttg
     3121 tcatgatctg caacctttct cgcgctgggc gggccgtgct ggtgcgggcg gcccgccggt
     3181 ccatggtgga acgtgacgct ggccgtgcag ccggtcgcct acacgcgcga ctgcccaggc
     3241 gcgggcgcgg tccgctcgct cgtggcctcg gcggggtcac gcggcgggcg cgcgatgaac
     3301 ggcagcacgg cgctgacgag cattacaacg gccacgatgg tcagggcggt actgtatttg
     3361 cccgtgttat ccttcacttg cgcgatgagg atcggtccca gcacgccccc cgccgaccac
     3421 gcgctcaaca tgaggccgta gatgcgcccg gcgaacttgg ggccgaagta gtcggcggcg
     3481 aaggcgggca tggtgccgaa gccgccgccg tagcacaagg ccacgatcat gaagaacacc
     3541 gacaacagga agaagtcggt gatggcgggc atgatcagga atagcacgaa ctggatcagg
     3601 aacatcgcca ggaagaccca gcgccgcgtg atcaggtcgg agagccaggc ccagaacagc
     3661 cgccccgcgc cgttgaagat cgagatcagg atgacgatgc tgctcgccgt ggtggcgtcc
     3721 acgccggtga tctcctgcgt catcggcgcc gcctgcgaga tcaccatgac cccggcggtg
     3781 acgttcagga agagcatggc ccagagcacg taccactgcc aggtgcgcag cgactcctgg
     3841 agcgtgaagt ctttacccgc gcgctggctg gcctgcgtgg cggtgggcgt ccagccggga
     3901 ggcgtgtagc ccgcgggcgc gacccggtag aattgcgcgg cgcccgcgac cataatcagg
     3961 tagacgatgc ccaggataat aaacgtggta tagacgccga cgccgctgat gaggccgggg
     4021 gcgatgaggg tcgtgaccac ggcgccaccg ccgaacccgg ctacggccaa cccggtgatc
     4081 agcccgcgct tgtcggggaa ccacttgatc agcgtggcga gcggcacgat gtagccgagg
     4141 cccatgccga tgccactgag caccccgtag gtcagccaca gcccccacag gctctggatc
     4201 agcccggccc cgatgaaccc gatcccatag aagaggccgg ccacggtggc gacggcccgt
     4261 ggtccgtact tatccagata ggtaccgccg acgatcgcgc cgacgccca
//
LOCUS       DASUYC010000562         2817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_464376,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000562 DASUYC010000000
VERSION     DASUYC010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464376"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VF276_09240"
     CDS             <1..474
                     /locus_tag="VF276_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX6607082.1"
                     /translation="ILGMGDVMTLIERAQSEYDMDQAKAMEKKLRTATFDLDDFLNQL
                     QMVKRLGPLQQVMEMIPGMGAAMRQSDVTVEEDDLKHIEAIIRSMTPKERHQPQIIDS
                     SRRRRIARGSGVTTQEVNMLLNQFKQMQKMMKQLGGWDGKGKPPRNLRGLMNMFR"
     gene            686..1042
                     /locus_tag="VF276_09245"
     CDS             686..1042
                     /locus_tag="VF276_09245"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6607083.1"
                     /translation="MLEGLNFVLVQVPDAQAAREFYADKLGLQVAEHMGGFVQFQPQD
                     GGAIFALEETPGAGPVQSVELWWQVDDADKLYRALQGQGVEILSPPRDEPFGRALEVK
                     DPAGHTVHMYQPPPRG"
     gene            complement(1193..>2817)
                     /locus_tag="VF276_09250"
     CDS             complement(1193..>2817)
                     /locus_tag="VF276_09250"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX6607084.1"
                     /translation="LYVQDVGGNENWRLYAVNLDSGEIRDLTPFESVQTQVIAQEKHL
                     PNELLIGLNKDNPQVHDIYRVDLPTGELTQLAKNPGTFMGWLPDTDLQVRAALAATPD
                     GGSELMVRDDETSDWRMLVAWNPDDTLSSEPLSFSKDGQSMLLRDSRDANAARLVRLD
                     LAGGDTQLLAEDPQYDVGQVVLHPDTYEVQMVGFARARLEWTVLDPAIEDDIAAIREL
                     HAGDFQIVSRDHADQTWLVAFIVDNGPVAYYTYDRATRKGAFLFENQPELAKYTLATM
                     TPVEFQSRDGRTIHGYLTLPPGQEGKNLPMVLDVHGGPWGRNAWGYDPEAQWLANRGY
                     ACLQVNFRGSTGYGKDFLNAGDREWGGKMHDDLVDAVQWAVDQGIADPARVAIYGGSY
                     GGYAALVGATFTPDLFCCAVDIVGPSNLVTLLKSIPPYWSTMRATMYKRVGNPDTEPA
                     FLESRSPLFKADRIKIPMLIAQGANDPRVAQAEAEQIVEAMQQNGVNYEYMLFPDEGH
                     GFAKPENRIKFYTAAEKFLAAHLGGRAEEAAA"
BASE COUNT          474 a          883 c          963 g          497 t
ORIGIN      
        1 atcctgggca tgggcgacgt gatgacactg atcgagcgcg cccagtccga atacgacatg
       61 gaccaggcca aggccatgga gaagaagcta cgcacggcca ccttcgacct ggacgatttc
      121 ctgaaccagt tgcagatggt caagcggctc ggcccgttgc agcaggtcat ggagatgatc
      181 cccggcatgg gcgcggccat gcgccagagc gacgtgacgg tggaggagga cgacctgaag
      241 cacatcgagg ccattatccg ctcgatgacg ccgaaggagc gccaccagcc gcagatcatc
      301 gacagcagcc ggcgccgccg catcgcccgc ggcagcggcg tgaccacgca ggaagtgaac
      361 atgctgctca accagttcaa gcagatgcag aagatgatga agcaactggg cggttgggac
      421 ggcaagggca aaccgccccg caacctgcgc ggcctgatga acatgttccg ttgatccgcc
      481 tcgcctttct aacgaatccc ctgcccgtgg gccatgcatc tgcatggccc gcgggggctt
      541 tccgggccat cccccctggc acaacagcat ccgaaagtcc cgaatcgctg ctgacatagg
      601 gctgtcactg cgctatgcga tactggtggt gggttaggaa acaggcccac cggctgatag
      661 tatcgcgagt gaaaggaagc gacaggtgct cgaaggacta aattttgtgc tggtgcaggt
      721 gcccgacgcg caggcggcgc gcgagttcta cgcggacaag ctgggcctcc aggtagcgga
      781 gcatatgggc ggctttgtgc agttccagcc ccaggacggc ggggccatct tcgccctgga
      841 ggaaacgccc ggcgccggcc cggtgcagag cgtggaactc tggtggcagg tggacgacgc
      901 cgataagctc tatcgcgccc tacaggggca aggggtcgag atcctcagcc cgccgcgcga
      961 cgagccgttt ggccgggcgc tggaggtgaa ggacccggcg ggtcacaccg tccacatgta
     1021 ccagccgccg ccccgcggct gatcgccagg gtatgcgaag ggggcgctcc cgcgcggggg
     1081 cgcccccttt cgtgtttgtt actctgagcg cagcgaagag tctcgtctcg tgcaggtggc
     1141 gctgagatgc ttcactgcgt tcagcaggac gcggcagcga cgctaggtct acttacgccg
     1201 ccgcttcctc cgcgcggccg cccaggtgcg cggccaggaa cttctcggcg gcggtgtaga
     1261 acttgatccg gttctcgggt ttggcgaagc cgtggccctc gtccgggaag agcatgtatt
     1321 cgtagttgac gccgttttgc tgcatcgcct ccacgatctg ctcagcttcg gcctgcgcca
     1381 cgcgcgggtc gttggcgccc tgcgcgatca gcattgggat cttgatgcgg tccgccttga
     1441 acagcggcga gcgcgattcc aggaacgccg gttcggtgtc ggggttgccc acgcgcttgt
     1501 acatcgtggc gcgcattgtg gaccagtagg gcgggatgct cttgagcagc gtcaccaggt
     1561 tgctggggcc gacgatgtcc accgcgcagc agaacaggtc gggcgtgaag gtcgcgccca
     1621 ccagggccgc gtagccgccg taggagccgc cgtagatggc gacccgcgcc gggtcggcga
     1681 tgccctggtc cacggcccac tgcacggcgt cgaccaggtc gtcatgcatc ttcccgcccc
     1741 attcgcggtc gcccgcgttc aggaagtctt tgccgtagcc ggtcgagccg cggaagttga
     1801 cctgcaaaca ggcgtagccg cggttcgcca gccattgcgc ctcgggatcg tagccccagg
     1861 cattgcgccc ccaggggccg ccgtggacat cgagcaccat cggcagattc ttgccctcct
     1921 ggccgggcgg caaggtgaga tagccatgga ttgtccgccc gtcgcgcgac tggaactcga
     1981 cgggcgtcat ggtcgccagc gtgtatttcg ccaactcggg ctgattctcg aacaggaagg
     2041 cgcctttgcg cgtggcgcgg tcataggtat aataggcgac aggaccatta tccacgatga
     2101 aggccaccag ccaggtctgg tcggcgtggt cgcggctgac gatctggaag tcgcccgcgt
     2161 gcaactcgcg gatggcggcg atgtcatctt cgatggccgg gtcgagcacg gtccactcca
     2221 gccgggcgcg cgcgaagccc accatctgga cttcgtaggt gtcggggtgc agcaccacct
     2281 ggcccacgtc gtactgcggg tcctcggcca gcagttgcgt gtcgccgccg gcgaggtcca
     2341 ggcgcaccag ccgggcggcg ttggcgtcgc gggaatcgcg gagcaacatg ctttggccgt
     2401 ccttcgagaa gctgagtggc tcgctgctca gcgtatcgtc ggggttccag gccacgagca
     2461 tgcgccagtc gctcgtctcg tcgtcgcgga ccatgagttc ggacccgccg tcaggggtgg
     2521 cggccagggc ggcacgcacc tgcaggtcgg tatcgggcag ccagcccatg aacgtgccgg
     2581 ggttcttcgc cagctgggtt agttctccgg tcggcagatc gacccggtag atgtcgtgga
     2641 cctggggatt gtccttgttg aggccgatca gcagttcgtt cgggagatgc ttctcctggg
     2701 cgatgacctg ggtttgcaca ctctcgaacg gggtcagatc gcggatctcg ccgctgtcga
     2761 ggttgacggc gtagagccgc cagttctcgt tgccgcccac gtcctggaca tagagca
//
LOCUS       DASUYC010000563         3527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_226583,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000563 DASUYC010000000
VERSION     DASUYC010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226583"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="VF276_09255"
     CDS             complement(<1..800)
                     /locus_tag="VF276_09255"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX6607085.1"
                     /translation="MQSANPDHEGGNSAGSMRALLIGVDAYNFVNPLSGCVNDILAVR
                     KFLVDNARIPPEQITMLLAPGRDAQLGPDLGTFESPTKNNILAAFKTLAEQTASGDQV
                     VVYYAGHGVQITNPQNSREHIGAIVAVDAQVNGTNFVINRELNEGIQRVVGAGAQVTV
                     ILDSCHSGGATRDISTDPAAPAVREIILDPQKLDWAAFQAEHGLDGNGAGDGATRDIA
                     GGSGWVPSLAQGQELIVLSGCRDVQTSREYTPPGTNERHGALTYFFLDA"
     gene            1147..1572
                     /locus_tag="VF276_09260"
     CDS             1147..1572
                     /locus_tag="VF276_09260"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HEX6607086.1"
                     /translation="MTQSPFDTSKPAHVHALERLQGDIIIWLSTVRPDGRPHLVPVWF
                     LWDEQTILIFSKPDQKIRNLRQNPAVVLGLDDTDTGEDVVLIEGTAELLPAGQVDTTL
                     PAYAAKYTPKLTGMGWTPASMATEYTEPIRITPTRVRTI"
     gene            1734..3230
                     /locus_tag="VF276_09265"
     CDS             1734..3230
                     /locus_tag="VF276_09265"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002083.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX6607087.1"
                     /translation="MADMKIRVVDDALEKVDADALVLNWFTEDKATLPAAWKSLDGKL
                     GGDLAATLKGDEVRGALYEVEPVHTAGALKVRRVFLIGAGSRETFGVMQLRRVAAAGA
                     RAARNRGAKRVAFALDAPGLSAAAIGQAIVDGALTGPLEPDLYRTQEREQRKLTEVIL
                     VRGALDKKALQAGAERGERIADGVNFSRGLAEEPANIMTPLVAAERAQAMAKEVGLDC
                     QVLDEKELKALGMNSLLSVALGSQWPARVVVLTYKGDRGSKEMLGLVGKGVTFDTGGI
                     SIKPALDMHHMKYDMGGAAAVIGAMRAIAQLKPKANVIGVAGFVENMPSGTANKPGDV
                     VRAANGKTIEILNTDAEGRLVLADALDLARKRGATRLVDIATLTGAVVIALGHVATGV
                     MGEPQSWVDTVLQASGTAGERAWQLPLFPEYKEQIKSMIADIANTGGRPAGTITAALF
                     LQNFVDTTPWAHLDIAGTGWNERDVPYSGKGATGAGVRTFVELASPSQ"
BASE COUNT          642 a         1082 c         1194 g          609 t
ORIGIN      
        1 gcatcgagga agaaataggt cagcgcgccg tgcctttcgt tggtgccggg cggcgtgtat
       61 tcgcgcgagg tctgcacgtc gcggcacccc gagagcacga tcaactcctg gccctgggcc
      121 aggctgggca cccagccgct gccgcccgcg atatcgcgcg tggcgccatc ccccgcgcca
      181 ttgccgtcta gcccgtgttc cgcctggaag gcggcccagt caagtttttg cggatcaagg
      241 atgatctcgc gcacggcggg ggctgccggg tcggtgctga tgtcgcgggt cgccccgccc
      301 gagtggcacg agtcgaggat gacagtgacc tgggcgcccg ctcccaccac gcgctggatg
      361 ccctcgttca actcgcggtt aatgacaaag ttggtgccgt tgacctgggc atccacggcg
      421 acgatggcgc cgatgtgttc gcgtgagttt tgcgggttgg tgatttgcac gccgtgcccg
      481 gcgtaataga ccaccacctg atcgccggac gctgtctgct cggccagagt cttgaaggcg
      541 gccaggatgt tgttcttcgt gggcgactcg aacgtgccca ggtccggtcc cagttgcgcg
      601 tcccggcccg gcgccagcag cattgtgatc tgctcgggcg ggatgcgggc attatccacc
      661 aggaacttgc ggaccgccag gatgtcgttg acacagccgc tgagcggatt gacgaagttg
      721 taggcgtcga cgccgatgag cagggcacgc atgctgcccg cggagttgcc gccttcatgg
      781 tccggattcg ctgattgcat cgccgggtcc tttcactatg tgccgcgccg ggcatgggga
      841 tccagatcga ctgggaacgg ggttggggtg gagcggaggt gggtctgccc cgatcatatc
      901 atatgatccg gctttgggca acgggtcgat cctgagcatt catgcagaaa ttcaccgcga
      961 aggaaagaag cacgcgaaga ttatcgatat cagacttgtt cttcgcccat gtgcgttttg
     1021 gccggatcac ccgtctgggg cggatgtatc gcccggcact gtggccgccc ggcgcgcgtg
     1081 ctatactggg cgtggcgcgg cgccgcagga ggcgcatcgc gacgctgcat gggaggaata
     1141 cggacaatga cccaatcgcc ctttgacacg agcaaacccg cccacgtcca cgcgctggag
     1201 cggctgcaag gcgacattat catctggctg agcacggtac ggcccgacgg gcggccccac
     1261 ctggtgcccg tctggttcct gtgggatgag cagacgatcc tcatcttcag caagcccgac
     1321 cagaagatcc gcaacctgcg gcagaacccg gcggtggtgc tcggcctgga cgacaccgac
     1381 accggcgaag acgtggtgct gatcgagggc acggcggaac tgctacccgc cgggcaggta
     1441 gatacaacgc tgcccgccta cgccgcgaaa tacacaccca agctgaccgg catgggctgg
     1501 actcctgcct cgatggcgac cgagtacacc gagcctatcc gcatcacgcc gaccagggtc
     1561 cgcaccatat aggccggcct cggcggcgcg cctggcacga aacttggtga gccaccgaga
     1621 tgtcgcgttt gttgacagcg ttgacagttt ttgtcgcggg atgtatgcta ataggcgtgt
     1681 ctcgcacacc aatttgatcg ctgtagttgt ctcgcaagag gagtgctcta ctcatggcgg
     1741 atatgaagat ccgcgtcgtc gacgacgcgt tagagaaggt cgatgccgac gccctggtgc
     1801 tcaactggtt cacggaagat aaagcgacgt tgcctgcggc gtggaagagc ctggatggca
     1861 agctaggcgg ggatctggcg gccacactta agggcgacga ggtgcgcggc gcactgtacg
     1921 aagtcgagcc ggtgcacacg gcgggagccc tgaaggtgcg tcgcgtcttc ctcatcggcg
     1981 cgggcagccg cgagacgttc ggcgtcatgc agttgcgccg agtggccgcc gccggggccc
     2041 gcgccgcgcg caaccgcggc gccaagcgcg tggcctttgc cctcgatgcg cccggcctga
     2101 gtgccgccgc gatcgggcag gcgatcgtgg atggcgcgct gaccggcccg ctggagcccg
     2161 atctctaccg cacgcaggag cgcgagcagc gcaagctgac cgaggtgatc ctggtgcgcg
     2221 gggcgctgga taagaaggcg ctgcaggcgg gcgccgagcg cggcgaacgc atcgcagacg
     2281 gcgtgaactt cagccgcggg ctggctgaag agccggccaa tatcatgacc ccgctggtgg
     2341 ccgccgagcg cgcccaggcc atggcgaagg aagtcggcct cgattgccag gtgctcgacg
     2401 aaaaggaact gaaggcgctg ggcatgaact cgctgttgag tgtggccctg ggcagccagt
     2461 ggcccgcccg cgtggtggtg ctgacctata agggcgaccg tggcagcaag gagatgctgg
     2521 gcctggtggg caagggcgtg accttcgaca ccggcggcat ttccattaag ccggcgttgg
     2581 atatgcacca catgaaatat gacatgggcg gggcggcggc ggtgatcggc gccatgcgcg
     2641 ccatcgcgca actcaagccc aaggccaatg tcatcggcgt ggccggcttc gtggagaaca
     2701 tgcccagcgg cacggccaac aagccgggcg acgtggtccg cgccgccaac ggcaagacca
     2761 tcgagatcct caacaccgac gccgaggggc gcctggtgct ggccgacgcc cttgacctgg
     2821 cccgcaagcg cggcgccacc cggctggtcg acatcgccac gctgaccggc gcggtcgtga
     2881 tcgcgctggg ccatgtggcg accggcgtga tgggtgagcc gcaaagctgg gtggacacgg
     2941 tgttgcaggc atcgggcacc gcgggcgagc gcgcctggca gttgccgctc ttccccgaat
     3001 acaaagagca gatcaagagc atgatcgccg acatcgccaa taccggcggg cgcccggcgg
     3061 gcacgatcac ggcggcgttg ttcctgcaga actttgtcga taccacgccg tgggcccatc
     3121 tcgacatcgc gggcacgggc tggaacgagc gcgatgtgcc ctattccggc aaaggcgcca
     3181 ccggcgccgg ggtgcgcacc ttcgtcgaac tggcgagccc cagccaatag gccccggccc
     3241 ggtgtatccg gcccttgggc cagggcgtcc taccagcaca ccgcgcgtat accgttaccc
     3301 ccgacacggg gggtaacggt ttttgttagt ggatctagcg gtatattcgt ttcttgtgct
     3361 ggtgcacaaa aattgctggt cacgtgatac atgcacaaag gtatctgttc agtaaaccgg
     3421 tgaatatgca aacgcactaa tattggtaag ttaggcagca ggtttgtctg gcgaagcgac
     3481 ataccatatc gcaaaataac atgctatact ataacccata agaatgc
//
LOCUS       DASUYC010000564         2168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_956665,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000564 DASUYC010000000
VERSION     DASUYC010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956665"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VF276_09270"
     CDS             complement(<1..545)
                     /locus_tag="VF276_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019507208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6607088.1"
                     /translation="MGYSTQVTQIDNTTEIFNQYRGLLFSIAYRMLGSGTDAEDIVQE
                     AFIRWMEARAEDVQSPRAYLCSIVVRLCIDQLRSARAQREVYVGPWLPEPIPTGPRPE
                     LAQTALLAESLSFAFLVMLENLKPLERAVFLLREVFEYDYAEIAEIVGKSEANCRQIL
                     HRAQQRLGERRPRFAVSHEQQ"
     gene            complement(629..1966)
                     /locus_tag="VF276_09275"
     CDS             complement(629..1966)
                     /locus_tag="VF276_09275"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6607089.1"
                     /translation="MKSQLTETDVVIVGGGLAGLSAACYLARGGVAVTLFEKAAHLGG
                     RAVTQMYDGYAFNRGVHALYCGGATSEVLQELGIPYSGHGPQDLFALRKGTLYPLPAD
                     PITMLRTDLLDLGGKWELLRWFAGLPRLNAAEFGRVSVQEWINRTVRRPAVRQLVSAV
                     ARTAVYSAALDQVSADVFIVKMQLQLKAGIKYIDGGWQTLVDGLRRAAEQAGAQIVSD
                     TRVTAVEYGDGAVQGVRLGDGSLVRAAAVILATSPQDAVRLVDGGAYPALRNIVDGLV
                     PAQIACLDVALRRLPDPRHPIVQDLERPRFLTTQSQFAHIAPEGGALIHTFKQLDPAH
                     PTDPHADEHELEDLLDRVQPGWREVLIKRVYLPRIDAIGMLPLAATGGYAGRPGPRVP
                     GLAHLYLAGDWIGGGFLADASLGSARQVARLLLDGELATARATAHPQPVSAGR"
BASE COUNT          354 a          746 c          720 g          348 t
ORIGIN      
        1 tcctgctgct cgtgcgagac ggcgaagcgc gggcggcgct cccccaggcg ctgctgcgcc
       61 cggtgcagga tctggcggca gttggcttcg ctcttgccca cgatctcggc gatttccgcg
      121 taatcgtact caaacacctc gcgcaacaag aacaccgcgc gctccagcgg cttgaggttt
      181 tcgagcatga ccagaaaggc aaaggacagc gactcggcca gcagggccgt ctgggccagt
      241 tcggggcgcg gcccggtggg gatcggttcg ggcagccagg ggccgacgta gacctcgcgc
      301 tgggcgcgcg ccgagcgcag ttggtcgatg cacagccgca ccacgatgga gcacaggtag
      361 gcgcgcggcg actgcacgtc ctccgcgcgg gcttccatcc agcggataaa cgcttcctgc
      421 acgatgtctt cggcgtccgt gccgctgccc agcatgcggt aggcgatgga gaacagtagg
      481 ccccggtact ggttgaatat ctcggtggta ttgtcgatct gtgtcacctg cgtactatac
      541 cccatttccg gtcggatgca aagaagaacg aacgggatat gtctacaagg cgggcaaggc
      601 cactaccctg cccgcctcgc ggtgatgttt agcgcccggc actcacaggc tgcgggtgcg
      661 ccgtggcccg cgcggtggcg agttcgccat ccagcagcag gcgcgccacc tgtcgcgcgc
      721 tgcccaggct ggcgtcggcc aggaagcccc cgccgatcca gtcgcccgcc aggtagagat
      781 gcgccaggcc gggcacccgt ggaccgggcc gtccggcgta cccgccggtg gccgccaggg
      841 gcagcatgcc gatggcgtcg atgcgcggca ggtagacgcg cttgatcagc acctcgcgcc
      901 agccgggctg cacccggtcc agcagatctt ccaactcgtg ctcgtccgcg tgggggtcgg
      961 tggggtgcgc cgggtcgagt tgcttgaacg tgtggatcag cgccccacct tccggcgcga
     1021 tgtgcgcgaa ctgcgattgc gtggtcagga agcgcgggcg ctccagatcc tgcacaatcg
     1081 ggtggcgcgg gtcgggcagg cgacgcagcg ccacgtcgag acaggcgatt tgtgcgggca
     1141 ccagcccatc aacgatgttc cgcaacgccg ggtaggcccc gccgtcgacc agccgcacgg
     1201 catcctgcgg gctggtggcg aggatcaccg cggcggcgcg taccagactg ccgtcgccca
     1261 ggcgcacacc ctggacggcc ccgtcgccgt actcgaccgc cgtcacccgt gtgtcgctca
     1321 cgatctgcgc gcccgcttgc tcggcggccc ggcgcaggcc gtctaccagc gtctgccagc
     1381 cgccgtcaat atacttgatg ccggctttga gttgcagttg catcttgacg atgaacacat
     1441 ccgcgcttac ctgatcgagc gcggcgctgt agaccgccgt gcgcgcgacc gcgctcacca
     1501 gttggcgcac cgccgggcgg cggaccgtcc ggttaatcca ctcctgtacg ctcactcgcc
     1561 cgaactccgc ggcgttgagc cggggcagcc cggcgaacca gcgcaagagc tcccacttgc
     1621 cgccgagatc gagcagatcg gtcctcagca tcgtgatcgg gtcggcgggc agcggataga
     1681 gcgtgccctt gcgcagcgcg aagagatctt gcggaccgtg gccgctgtag gggatgccca
     1741 gctcctgcaa cacctcgctg gtcgcgccgc cgcaatacag cgcgtggacg ccccggttga
     1801 aggcgtagcc gtcatacatc tgcgtgaccg cgcgcccgcc cagatgcgcc gccttctcga
     1861 acagcgtcac ggccacgccg ccccgcgcca ggtaacacgc cgcgctcaac ccggccaggc
     1921 caccgcccac gatcacgaca tcggtttcag tcagttgcga tttcatagtg ttatgcctcc
     1981 tgcgttttgc cccgatgggc gaattgtcac agacaagacg gaaacgcacc tccgtttgtg
     2041 acaaaatcgc ggcgccgtcc agggcgtcag cgagaaaggc ccgaagtgag cagagatccg
     2101 ccgcaggggc tatccaaccg agggtgttgg catagcccct gctggctggt cgctataacc
     2161 ggcgcatc
//
LOCUS       DASUYC010000565         3647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1764193, whole genome shotgun sequence.
ACCESSION   DASUYC010000565 DASUYC010000000
VERSION     DASUYC010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1764193"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="VF276_09280"
     CDS             <1..930
                     /locus_tag="VF276_09280"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6607090.1"
                     /translation="YLGNALIGVGGAVGIVALLGLSTIGSPDFDVATIANSLIQISIV
                     MLLLSATSGIVLRERASRRHAEALRAGLAASHQELQAGAAQALAAVQARNQLAWDIHD
                     GLGHYLTVINVQLEKALAFRAVDTAVADAALHDARLLTGEALQEVSDSLGAWDADTAF
                     SLRAALTVLGQRLDGGPVILDLQILGDESGFSRPALMTLFRAAQEGLTNIQRHAAATH
                     AWVTLRFTPHEAVLDIRDDGRGLAAPAGGNRAHGSGLQSMRERLELVGGALAVESATG
                     QGTRLLITVPRDPQPHLAPAPLATPLPTAGVPV"
     gene            927..1625
                     /locus_tag="VF276_09285"
     CDS             927..1625
                     /locus_tag="VF276_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015100104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6607091.1"
                     /translation="MTAPPNPPESPVGVLVVDDQRLMRDGIASLLSLQPGIVVLGTAA
                     NGQEAVAQAEALRPDVILMDVRMPVLDGVAATAQVRRAVPTCQVLMLTTFDDEEYIVA
                     ALRAGASGYLLKDLPAADLAQAILAAHKGIYQIDPAVAGKMVAALTGSSPPAPAPTPG
                     ASVPPAARPDGLSSREIEVLRLIAQGASNREIAETLVISEGTVKNHISNILGRLGLRD
                     RTQAALYARENGLI"
     gene            complement(1702..2244)
                     /locus_tag="VF276_09290"
     CDS             complement(1702..2244)
                     /locus_tag="VF276_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX6607092.1"
                     /translation="MAEPHPAPSPEEVHTAIVRRLEAAGVPFTIHTHEVARTVADAEA
                     RLPFPKEQFLKTVVFRLKAGGWILVALQGQDQVDYRRLADALGVKRGDLLRPPPEEVE
                     AALGYQIGGVGPIPLAPDTRVLFDEAALPMATVYCGAGRNDRTLEIGLADLIRAVAGP
                     VAPLARERADSPSAADPGAS"
     gene            complement(2237..>3647)
                     /locus_tag="VF276_09295"
     CDS             complement(2237..>3647)
                     /locus_tag="VF276_09295"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX6607093.1"
                     /translation="LLAGFVLVAVLAAAFLSHSGTLAEAVDDQLRDAAGRVAPVVRRA
                     NGSGVPDSTALQPVTHWTDAEIWVRYLGNDGRLMKPSPNATDRPSLFMTLPPGAESAY
                     VDGRDGPERLRGYILRVYRDGTPVGYVEAGTSLASIDRLNAYFAALLVAISVGGLLLT
                     AGAGWAIAAGALRPVSTIIATAREITGTRSFARRVPAAARRDELGTLTETLNNMLASL
                     EAAYAVQRRFVDDAAHELRAPITTIQGNLALLREPGSLPPDEQAAVLADLQSEVQRLA
                     RLVNGLLALARADAGQTGRAEVVELDTVLTTTYHRLLGLAPAAPVHIRGLEPLCVRGD
                     RDRLLQLALALGENAVRYTPAGGAVDLSLARDGDSAVLAVRDTGVGIPPADLPFVFDR
                     FYRGDAARDLHREGTGLGLPIARWIVEMHQGTLAVESPPGGGTTFTVRLPLVPCAAPP
                     SAADGADAAPARQEVPVDG"
BASE COUNT          557 a         1290 c         1311 g          489 t
ORIGIN      
        1 tacctgggga acgcgctgat cggagtcggg ggggcggttg ggatcgtggc gctgctgggc
       61 ctgagcacca tcggctcgcc ggactttgat gtcgccacca tcgccaacag cttgatccag
      121 atcagcatcg tcatgctgtt actcagcgca acgtccggca tcgtgctgcg ggagcgcgcc
      181 agccgccggc acgccgaggc gttgcgcgcc ggcctggcgg cctcgcacca ggagctacag
      241 gcaggcgcgg cgcaggcgct ggccgcggtg caggcgcgca accagctcgc ctgggatatc
      301 cacgacggcc tgggccacta cctgacggtc atcaacgtcc aactcgaaaa ggcgctggcc
      361 tttcgcgcgg tcgatacggc ggtggccgat gccgcgctgc acgatgcccg cctgctgacg
      421 ggcgaggcgt tgcaggaggt cagcgactcg ctgggggcct gggacgccga taccgcgttc
      481 tcgttgcggg ccgcgctaac cgtgctgggg cagcgcctgg atggcgggcc cgtgatcctg
      541 gatctgcaaa tcctgggcga cgagagcggg ttctcccgcc cggcgctcat gaccctcttc
      601 cgcgccgccc aggaagggct gaccaacatc cagcgccacg ccgccgcgac gcacgcctgg
      661 gtcaccctgc gcttcacgcc gcacgaggcg gtgctcgaca tccgcgacga cggacgcggc
      721 ctggccgccc cggcgggcgg gaaccgcgcc cacggctcgg gcttacagag catgcgcgaa
      781 cggctggaac tggtgggcgg cgcgctggcg gtggagagcg caaccgggca gggaacccgg
      841 ctgctcatca cggtgccccg cgatccccag ccgcacctcg ccccggcgcc gctcgcgacc
      901 ccgctaccta ccgcgggggt gccagtatga cggcgccacc caatccgccg gagtcgccgg
      961 tcggggtgct ggtggtggac gaccagcggc tgatgcgcga cggcatcgcg tcgctgctga
     1021 gcttgcagcc cggcatcgtc gtgctcggca ccgccgcgaa cgggcaggaa gccgtagcgc
     1081 aggccgaggc cctgcgaccg gatgtgatcc tgatggatgt gcggatgccg gtgctggatg
     1141 gggtggcggc cacggcccag gtgcgccgcg cggtgcccac ctgccaggtg ctgatgctga
     1201 ccacctttga cgacgaggaa tacatcgtcg cggccctgcg cgccggggcc agcgggtatc
     1261 tgctgaagga cctgcccgcc gccgatctgg cgcaggccat cctggcggcg cacaaaggga
     1321 tctaccagat cgaccccgcc gtggcgggca agatggtggc ggcactgacc ggcagcagtc
     1381 ccccggcgcc tgccccaacg ccgggcgcgt ccgtgccgcc cgccgcgcgc cctgacgggc
     1441 tgagcagccg ggaaatcgag gtgctgcgcc tgatcgccca gggcgccagc aaccgcgaga
     1501 tcgccgagac actggtgatc agcgagggca cggtcaagaa ccacatctcc aacatcctgg
     1561 ggcgcctggg gctgcgcgac cgcacccagg cggccctgta cgcccgcgaa aacggtctga
     1621 tctaaaccaa ataccagggg taccggactg cgccggtctc ttgatggagc tagccgcctc
     1681 tgtttaagct gagatctagc ttcaggatgc gccggggtcg gcggcggacg gggaatcggc
     1741 acgttcgcgg gccagaggcg ccaccggccc ggctaccgcg cgaatgagat cggcaagccc
     1801 gatctccagc gtgcggtcgt tgcggcccgc gccgcaatag accgtcgcca tcggcagcgc
     1861 ggcctcatcg aacagcacgc gggtatccgg ggcgagcggg atggggccga ccccgccgat
     1921 ctggtagccc agcgcggcct cgacctcctc cggcggcggg cggagcaagt caccgcgttt
     1981 gacgccgagc gcatcggcca accggcggta atcgacctgg tcttgccctt gcagcgccac
     2041 gagaatccag ccgcccgcct tcagccggaa caccaccgtc ttcaagaatt gctctttggg
     2101 gaagggcaac cgcgcctcgg catcggccac ggtgcgcgct acctcgtggg tgtggatcgt
     2161 gaacggcacg ccggccgcct ctaagcgccg gacaatcgcc gtatgcactt cctccggaga
     2221 tggggcggga tgcggttcag ccatcgacgg gcacctcctg ccgggcgggt gcggcgtcgg
     2281 cgccgtcggc agcggacggc ggagcagcac agggcaccag cggcagccgg acggtgaagg
     2341 tagtgccgcc accgggcggg ctttccacgg ccagcgtgcc ctggtgcatc tcgacgatcc
     2401 agcgggcgat gggcagtccc agcccggtcc cctcgcggtg caggtcgcgg gcggcgtcgc
     2461 cccggtagaa gcggtcgaac acgaagggca ggtcggcggg cgggataccg acgccggtat
     2521 cgcgcacggc cagcacggcg ctgtcgccgt cgcgggccag gctgagatcg accgccccgc
     2581 ccgccggggt atagcgcacg gcattttcgc ccagggccag cgccagttgc agcagccggt
     2641 cgcggtcgcc gcgcacacac agcggttcga ggccgcggat gtgcaccggc gcggcgggag
     2701 ccagccccag caagcggtga tacgtggtcg tcagcacggt gtccagttcc acgacctcgg
     2761 cgcgcccggt ttgcccggca tcggcgcggg ccagcgccag cagaccgttg accaggcggg
     2821 ccaggcgctg gacctcgctc tggagatcgg ccagcaccgc ggcctgctcg tccggcggca
     2881 ggctgcccgg ctcgcgcagc agggccaggt taccctggat ggtggtgatc ggcgcgcgca
     2941 gttcgtgcgc ggcatcgtcc acgaagcggc gctgcacggc gtaggcggcc tccagactcg
     3001 ccagcatatt gttgagtgtc tcggtcagcg tgcccagttc atcgcggcgc gccgccgccg
     3061 gcacccgccg ggcaaagctg cgcgtgccgg tgatctcgcg cgcggtggcg atgatcgtgc
     3121 tgacggggcg cagggcgccc gccgcgatgg cccagcccgc ccccgcggtg agcagcaacc
     3181 cgccgaccga gatcgccacc aggagcgcgg caaaataggc gttcaaccgg tcgatgctcg
     3241 ccagcgaagt accggcttcg acatagccga ccggcgtccc gtcccggtag acgcgcagga
     3301 tgtagccgcg cagccgctcg ggaccgtcgc gcccatccac gtaggccgat tccgcgccgg
     3361 ggggcaacgt cataaacagg gaggggcgat ccgtggcatt gggtgagggc ttcatcagcc
     3421 ggccatcgtt acccagatag cgcacccaga tttcggcgtc ggtccaatga gtcaccggct
     3481 ggagtgcggt ggagtcgggg acgccggacc cgttcgcgcg tcgcaccacc ggggccaccc
     3541 gccccgccgc atcgcgcaac tggtcgtcca cggcctcggc cagcgtgccg ctgtgcgaga
     3601 gaaaggccgc cgccagcacc gcgaccagca cgaacccggc cagcagg
//
LOCUS       DASUYC010000566         2739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_703730,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000566 DASUYC010000000
VERSION     DASUYC010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703730"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..1342)
                     /locus_tag="VF276_09300"
     CDS             complement(152..1342)
                     /locus_tag="VF276_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX6607094.1"
                     /translation="MATSDIYRDKLEQASTLLKQQDIDLWLLFVRETGTLNDPSLPLI
                     CDLSFTWETAVLISATGRHTVLAGLHDCDNVRATGLFPNVVPYTDSMVAPFREVLAGY
                     DPRRIALNYSPSDPSADGLTHGMWLLLGEMLAGTPYATRLESSETFLGRFRATKTPSE
                     LARMRAALRAAEEIWATTASFLKPGQSEREVAAFMHGELDRLRLWTSWERDFCPIVHA
                     GSDSSTGHARPGDRRIAPGRLVNIDFGVLKDGYCTDQQRMWYCRQLGEKTVPAEVTRA
                     FDTVVGAIQAAATVLRPGIRGYEVDAIARRFVTDAGYPEYQHALGHTVGRTTHDGGPL
                     LGPRWDRYGTTPEGIIEAGQIYTLELGVDTPQGYCGLEEEVLVTDHGCEFLSAPQMEL
                     MFIE"
     gene            1513..>2739
                     /locus_tag="VF276_09305"
     CDS             1513..>2739
                     /locus_tag="VF276_09305"
                     /inference="COORDINATES: protein motif:HMM:NF013377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HEX6607095.1"
                     /translation="MSESSYGTRRAIDELLAIFSCQWANGMLPQIRFMPGQHGYRPDA
                     DDWGVTEAISGPTALRTSGITQPPIMGLCAWEVFRKIGPEAQAAHAADFRTIAAGLER
                     FHDWLFRERDPWNEHLVLCLHPWETGTDNSPAFDWLIDSTRAYIEQSGLAVETFGRAD
                     TVHVNAAHRPTARDYYAYFGLMALFKKHHYDQRAIIAESPFLLQDVLFNSLLAVSLRA
                     LAQLEEALAEQDGADAGALREQANRNRERTAAMAGAMRDKFWDIADAFFYSYDSRGKR
                     QLPIPTVSGFMPLLAEIADAGQARRVLEHLHDPEEFASPVPIPSTSLDSPVFSSERYW
                     SGPSWPVTNWLVVRGLRERGAVADAEALRLSTLAMIAEGADPAAVRMAAMGVMELNSF
                     DDHFTTPSSQQYAHAWL"
BASE COUNT          426 a          901 c          952 g          460 t
ORIGIN      
        1 gccgatagtc tcgtagtata aaaactctgg cgtgttcata attccgcctg taggttatga
       61 tgccgacgtg tagggtgaaa caaacgagga ggaaccgggt cacccgattc ctccccgcta
      121 ctgatggccc gcgccgggct agtggtgcct gtcactcgat gaacatcagc tccatctgcg
      181 gggcggagag gaattcgcag ccgtggtcgg tgacgagcac ttcttcctcc aggccgcagt
      241 agccctgggg cgtgtccacg ccgagttcga gcgtgtagat ctgtccggcc tcgatgatgc
      301 cttccggggt ggtgccgtag cggtcccagc gcgggccgag cagggggccg ccgtcgtgcg
      361 tggtgcggcc cacggtgtgg ccgagcgcgt gctggtattc ggggtagccc gcgtcggtca
      421 cgaagcgccg ggcgatggcg tccacctcgt agccgcggat gccgggccgg agcacggtcg
      481 cggcggcctg gatcgcgccc actaccgtgt cgaaggcgcg ggtgacttcg gcgggcaccg
      541 tcttttcgcc cagttggcgg cagtaccaca tgcgctgctg gtcggtgcag tagccgtcct
      601 tgagcacgcc gaagtcgatg ttcaccaggc gaccgggggc gatgcgccgg tcgccggggc
      661 gggcgtggcc ggtgctggag tccgacccgg cgtggacgat ggggcagaag tcgcgttccc
      721 aggaggtcca gagccgcagc cggtcgagtt cgccgtgcat gaaggcggcc acctcgcgct
      781 cgctctggcc gggcttgagg aaggaggcgg tggtggccca gatctcctcg gcggcgcgca
      841 gggcggcgcg catgcgggcc aactcgctgg gcgtcttggt ggcgcggaag cggcccagga
      901 aggtctccga gctttccagg cgggtcgcat agggcgtccc ggccagcatc tcgcccagca
      961 gcagccacat gccgtgggtc aggccgtcgg cggaggggtc gctgggcgag tagttcagcg
     1021 cgatgcgccg cgggtcgtac ccggccagca cctcgcggaa gggggcgacc atgctgtcgg
     1081 tatagggcac cacgtttggg aacaggccgg tggcgcgcac attgtcgcaa tcgtgcaggc
     1141 ccgccagcac ggtgtggcgg cccgtggcgc tgatcagcac ggcggtttcc caggtgaagc
     1201 tgaggtcgca gatcagcggc aggctgggat cgttgagcgt gcccgtttcg cgcacgaaca
     1261 acagccagag atcgatgtcc tgctgcttga gcaacgtgct cgcctgctct agtttgtctc
     1321 gataaatgtc gctggtcgcc atggggtctc ctgtgccggt gctgagtatg gggcggggcg
     1381 ctgagtccgg caaagactca gggcacgcat cctgttgtac cacaactatg gacacaccgg
     1441 caagcggccg tcgcaaattg cttaggccgc ccgttcgccc tgtagagggt tcacgcgcag
     1501 aggagggaca tggtgagcga gtcgagctat gggacgcggc gcgcgattga cgaattgctg
     1561 gcgatctttt cctgccagtg ggccaacggc atgctgccgc agatccggtt catgccggga
     1621 cagcacggct accggcccga cgccgacgac tggggcgtca ccgaggccat cagcggcccg
     1681 acggcgctgc gcacgtcggg catcacccag ccgccgatca tgggcctgtg cgcctgggag
     1741 gtcttccgca aaatcgggcc ggaggcgcag gccgctcacg ccgccgattt ccgcaccatc
     1801 gccgccgggc tggagcgctt tcacgactgg ctcttccgcg agcgcgaccc ctggaacgag
     1861 cacctggtgc tctgcctgca cccctgggag accggcaccg acaactcgcc tgccttcgac
     1921 tggctgatcg acagcacgcg ggcctatatc gagcagagcg ggctggccgt cgagaccttt
     1981 ggccgcgccg acacggtcca cgtcaacgcc gcccatcgcc cgaccgcgcg cgactactac
     2041 gcctactttg gcctgatggc gctgttcaag aagcaccact acgaccagcg cgccatcatt
     2101 gccgaaagcc cgttcttgct gcaagacgtg ctcttcaaca gcctgctggc ggtgtcgttg
     2161 cgcgctctgg cccaactgga ggaggcgctg gccgagcagg acggggccga tgccggggcg
     2221 ctgcgcgagc aggccaaccg caatcgtgag cgcaccgccg cgatggccgg ggccatgcgc
     2281 gacaagttct gggatatcgc cgacgccttt ttctacagct acgatagccg gggcaagcgc
     2341 cagttgccca tccccaccgt ctccggcttc atgccgctgc tggccgagat cgccgatgcc
     2401 ggccaggccc ggcgggtact ggaacacctg cacgaccccg aggaattcgc ctcgccggtg
     2461 cccatcccct cgacttcgct cgacagcccg gttttcagtt ccgagcgtta ctggtcgggg
     2521 ccgtcgtggc ccgtgaccaa ctggctggtg gtgcgcggct tgcgcgaacg tggcgccgtg
     2581 gccgacgccg aagccctacg cctcagcacc ctggcgatga tcgccgaggg cgccgacccc
     2641 gccgccgtgc gcatggcggc catgggcgtg atggaactga acagcttcga cgatcacttc
     2701 accacgccct ccagccagca atacgcccat gcctggctc
//
LOCUS       DASUYC010000567         2200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_321869,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000567 DASUYC010000000
VERSION     DASUYC010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321869"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1224
                     /locus_tag="VF276_09310"
     CDS             <1..1224
                     /locus_tag="VF276_09310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6607096.1"
                     /translation="GGQQVLALPLLDEERLIGLALLAHQGVSGRDVRFLHTVAREMGT
                     ALRAMMGRQALLAQEQKLEAIVMGTTDAIIQVAPGGRVQDFNRAAEVLTGYTTADALD
                     QSCAEVLSCAEGAGCGGTCPFAQVLASREPIPYAELLVRAPHGPRHVAASVAALDLDP
                     HDAPGAVAILRDMTEQKQIEQMKSDFLITVSHQLRTPLALLRGYSETLRHLDLTPREQ
                     STCVTGIAEATERLARLVEQILDVTRIEDGRMELHREPVRLVDVVRRAITGLPYTAYR
                     SRVWAEVAPDLPLIAADPQRLEQVVLNLLDNALKYSSPAGPVLVRARAGAGAVELQVL
                     DEGIGVPAADQAGLFTKFQRAENARRLQVPGTGLGLFICRSIVEAHGGRISLESTPGA
                     GTCVTCRLPLYTEGT"
     gene            1228..1956
                     /locus_tag="VF276_09315"
     CDS             1228..1956
                     /locus_tag="VF276_09315"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6607097.1"
                     /translation="MAAILVAEDEAGLRDLIQLWLERAGYACHTAGSGRAALRLFYDT
                     HPDLILLDLALPEMDGWQLCERIREVSQVPIIMVTARAEEPAKVRGLQMGADDYVTKP
                     FGFPELLARVQAVLRRAGAGRPEHEVYHQGDLLLDPQAHRAMLAGADLKLTPTEYRLL
                     AFLMQHSGQLLTHRQILEGVWGPAYADDLDSLRIYIRNLRRKLDAGGGARYIATEHGL
                     GYRFLKTDATDVHREATKATKNDQ"
BASE COUNT          349 a          754 c          778 g          319 t
ORIGIN      
        1 ggcggccagc aggtgctggc cctgccgctg ctggacgaag aacggctgat cgggctggcg
       61 ctgctggcgc accagggggt gtcgggccgc gacgtgcgct tcttgcacac ggtggcccgc
      121 gagatgggca cggcgctgcg ggccatgatg ggccgccagg cgctgctggc ccaggaacag
      181 aagctcgaag ccatcgtcat gggcacgacc gacgccatca tccaggtggc gccgggcggg
      241 cgggtgcagg acttcaaccg cgccgccgaa gtgctcaccg gctacaccac cgcggatgcg
      301 ctcgaccagt cgtgcgccga ggtgctgagt tgcgccgagg gggcgggctg cggcgggacg
      361 tgcccgttcg cccaggtgct tgccagccgc gagccgatcc cctacgcgga actgctggtg
      421 cgcgcgccgc acggcccgcg ccacgtggcc gccagcgtgg ccgcgctcga tctcgacccc
      481 cacgacgcgc ccggcgccgt ggcgatcctg cgcgacatga ccgagcagaa gcagatcgag
      541 cagatgaagt ccgatttcct gatcaccgtg tcgcaccagc tacgcacgcc gctggcgctg
      601 ctgcgcggct acagcgagac gctgcgccac ctcgacctca ccccgcgcga gcagagcacc
      661 tgcgtgaccg gcatcgccga ggccaccgag cggctggcgc ggctggtcga gcagatcctc
      721 gacgtgacgc gcatcgagga cgggcgcatg gagctgcacc gcgagccggt gcgcctggtg
      781 gatgtcgtgc ggcgggccat caccgggctg ccctacacgg cttaccggtc gcgggtgtgg
      841 gccgaggtgg cgcccgacct gccgctgatc gccgccgacc cgcagcggct cgaacaggtg
      901 gtgctcaacc tgctcgacaa cgcgctcaaa tactcctcgc cggcggggcc ggtgctggtg
      961 cgggcgcggg ccggggcggg cgcggtcgag ctccaggtgc tggacgaggg catcggcgtg
     1021 cccgcggcgg accaggccgg gctgttcacc aagttccagc gggccgagaa cgcgcgccgc
     1081 ttgcaggtgc cggggacggg gctggggctg tttatctgcc gcagcatcgt cgaggcccac
     1141 ggcgggcgca tcagcctcga aagcacgccg ggcgccggga cgtgcgtcac ctgccggctg
     1201 cccctctaca cggaagggac ctgatccatg gcggcgatcc tggtggccga agacgaagcc
     1261 gggctgcgcg acctgatcca gctctggctg gagcgcgcgg gctatgcgtg ccacacggcg
     1321 ggcagcgggc gggcggcctt gcgcctgttc tacgacaccc accccgacct gatcttgctc
     1381 gacctggcgc tgcccgagat ggacggctgg cagctctgcg agcggattcg ggaagtctcg
     1441 caggtgccga tcatcatggt cacggcgcgc gccgaggagc cggccaaggt gcgcgggttg
     1501 cagatgggcg ccgacgatta cgtgaccaag ccgttcggct tcccggaact gctggcgcgg
     1561 gtgcaggcgg tgttgcgtcg cgccggggcc gggcgcccgg agcacgaagt ctaccaccag
     1621 ggcgacctgt tgctcgaccc gcaggcgcac cgggccatgc tggccggggc cgacctgaag
     1681 ctgacgccca ccgagtaccg cctgctggcc ttcctcatgc agcacagcgg ccagttgctg
     1741 acccaccgcc agatcctcga aggcgtctgg gggccggcct acgccgacga cctggactcg
     1801 ctgcgcatct acatccgcaa cctgcgccgc aagctcgacg cgggcggcgg cgcgcgctac
     1861 atcgccaccg agcacggcct gggctaccgc ttcctcaaga cggatgctac ggatgttcac
     1921 cgcgaagcaa cgaaggcaac gaagaatgac caatgagggg gtgtcccgct gagcagtctg
     1981 tcacatggtt tttgtcactc tgagcgcagc gaagagtctc gtctttctag ctacgagacg
     2041 gagattcttc gctccgctca gaatgacaat ttaagtgtga cacactactg agcggagcga
     2101 agagtcgcga cgcctcgcgc cggagacgag attcttcgct gcgctcagaa tgacacgttc
     2161 agtgtgaccg cctattcagc aaacccccca aaacccttcg
//
LOCUS       DASUYC010000568         2028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_103266,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000568 DASUYC010000000
VERSION     DASUYC010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103266"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..1085)
                     /locus_tag="VF276_09320"
     CDS             complement(105..1085)
                     /locus_tag="VF276_09320"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6607098.1"
                     /translation="MPADDPAGAVHIRPMALADIPAVVRLINAADAVDQADEGTSEAE
                     FRHWVDQSGGPGDRVVALDATGAMVGYGDVYHQPGDEGAWGWLLVHPAWRRHGIGMLL
                     AAQVLDRARRLGVAWIDYATDVRLHEANAWLARLGYEPVRTYMRLRLPPEVAVPAPVY
                     PAGFQPHTFRRNDLAAVRAILAAPFADHRNPQASAAGGDQAAEDLGQPWFDPRGLFVV
                     ETEESQMAGLCWAYINYDENRRRGESIGWVNDLEVARAYRRQGLGRALLLDGIHWLRG
                     QGMRAVDLWVDSGNREARSLYAALGFQVDKSVVDYRHFLRVGRPAESDAG"
     gene            complement(1075..>2028)
                     /locus_tag="VF276_09325"
     CDS             complement(1075..>2028)
                     /locus_tag="VF276_09325"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX6607099.1"
                     /translation="SDIHMSRDPHDGMVLLAEAGRLLAQAIRQINALPGLDCVLVTGD
                     LVDNGAPDELDDVLAQLTNLRVPWYAIPGNHDAAFPPDSDRLNRRRFYEHVAAATPAG
                     TDAYDGAAERGSWTILLKPGVRLIALDSNIPGDWSGEIDAPQLAWLETTLDAATEPLV
                     VLMVHHPLHKVFGGWERPEFAGQDWNKFFCNNGAEVRALLDRYPQVRLVLTGHDHVSR
                     VDQFKGRLHLAAPALSSYPLAFRTVHVHGIDRGWTFTWQTYTPADDALRAEGLAKLET
                     GTLALAFAPADPHRFAVLSEGQASDQQGRLQVRPRPVRNAR"
BASE COUNT          286 a          664 c          767 g          311 t
ORIGIN      
        1 cgcgggcata atcgtcgtcg tagtagaggg tgatcgtggg tgttgacact ggctgcctcc
       61 aggcggtagg tttattggcg taaagcaccg ggcgggttgg tgggttagcc tgcgtcgctc
      121 tcagccgggc gacccacgcg cagaaaatgg cggtaatcga cgacggattt gtccacctgg
      181 aagccgagcg cggcgtagag gctgcgggct tcccggttgc cgctgtccac ccagagatcg
      241 acggcgcgca tgccctggcc gcgtagccag tggatgccgt cgagcagcag cgcccggccc
      301 agcccttgcc gccggtaggc gcgggccact tccaggtcgt tcacccagcc gatggactcg
      361 ccgcggcgcc ggttctcgtc gtagttgata taggcccagc agaggcccgc catctggctc
      421 tcctcggtct ccaccacgaa caggccgcgc gggtcgaacc agggctggcc caggtcttcc
      481 gccgcctgat cgcctccggc ggcggatgcc tggggattgc ggtggtcggc gaagggcgcg
      541 gcgaggatgg cacggacggc ggcgaggtcg ttccggcgga aggtatgcgg ctggaacccg
      601 gcggggtaaa ccggcgccgg tacggcgacc tcgggcggca ggcgcaggcg catgtaggtg
      661 cgcaccggct cgtagcccag gcgggccagc caggcgttcg cttcgtgcag gcgcacgtcg
      721 gtggcgtagt cgatccaggc gacgcccagg cggcgggccc ggtcgagcac ctgcgcggcc
      781 agcagcatgc cgatgccgtg ccgccgccag gcggggtgga ccaggagcca gccccaggcg
      841 ccctcgtcgc ccggctgatg atacacgtcg ccgtagccga ccatggcgcc ggtggcgtcc
      901 agcgccacca cgcggtcgcc cggcccgccc gactgatcga cccagtggcg aaactcggct
      961 tcgctggtgc cttcgtcggc ctgatccacg gcgtccgccg cgttgatgag ccgcaccacg
     1021 gcggggatgt cggccagggc catcgggcgg atatggacgg ctccggcagg gtcgtcagcg
     1081 ggcattgcgt acggggcggg ggcggacttg caggcggcct tgctggtcgc tcgcctggcc
     1141 ctcggagagc acggcgaagc ggtgcgggtc ggcgggcgcg aaggccaggg ccagcgtgcc
     1201 ggtttccagc ttcgccagcc cctcggcccg cagggcgtcg tccgccgggg tgtaggtctg
     1261 ccaggtgaag gtccagccgc ggtcaatgcc gtgcacgtgg accgtgcgga aggcgagggg
     1321 atagctgctc agcgcgggcg cggccaggtg caggcggccc ttgaactgat cgacgcggct
     1381 cacatggtcg tggcccgtca gcaccagtcg cacctgcgga tagcggtcga gcagcgcgcg
     1441 cacctcggcg ccgttgttgc agaagaactt gttccagtcc tggccggcga attcggggcg
     1501 ctcccacccg ccgaagactt tgtgcagcgg gtgatggacc atgagcacga ccagcggttc
     1561 ggtggccgcg tcgagcgtcg tttccagcca cgccagttgc ggcgcatcga tctctcccga
     1621 ccagtcgccg gggatgttgg agtcgagcgc gatcaggcgc acgccgggtt tgagcaggat
     1681 ggtccaactc ccgcgctcgg cggccccgtc ataggcatcc gtgccggcgg gcgtagcggc
     1741 ggccacatgc tcgtagaagc gccgccggtt gaggcgatcc gagtcgggcg ggaaggcggc
     1801 gtcatggttg ccggggatag cgtaccaggg cacgcgcaga ttcgtcagtt gggccagcac
     1861 gtcgtccagt tcgtcgggcg cgccgttatc gaccaggtcg cccgtgacca ggacgcagtc
     1921 gagccccggc aaggcgttga tctggcggat ggcctgggcc agcaggcgcc cggcctcggc
     1981 cagcaggacc atgccgtcgt gcgggtcgcg gctcatatgg atgtcgct
//
LOCUS       DASUYC010000569         4387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1671675, whole genome shotgun sequence.
ACCESSION   DASUYC010000569 DASUYC010000000
VERSION     DASUYC010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1671675"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VF276_09330"
     CDS             complement(<1..248)
                     /locus_tag="VF276_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607100.1"
                     /translation="MYNVIAPRIVNNNQLGIWPYLLVESPMSQSTSREFFSEVKDWSK
                     RKHQILDMYLASASKILGSRWKTVVYLDGFAGRGTYGEG"
     gene            457..1857
                     /locus_tag="VF276_09335"
     CDS             457..1857
                     /locus_tag="VF276_09335"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX6607101.1"
                     /translation="MLVMKFGGTSVGSAGRIREVAGLVQRTAAEEPVVVVVSAVSGVT
                     DSLIAAARQAAEGRRLQAIQHAIGIEQRHTEILNALDLPAEERQSMATELRALSGDLE
                     NFIHSVGTLGELTQRTLDYISSMGERMSCRLLAATLRAAGQPAEAVDATKVVVTDNNF
                     GHAFPLMDPTRAQAEAVLAPRLREGRVPVVTGFIGATEAGIVTTLGRGGSDYSAAILG
                     SALGAREVVIWTDVDGVMTANPRVVPGARTLPRMSYLEAAEMSYFGAKVLHPKTIAPA
                     VEGGIPVRIKNTFAPDAPGTLITAEAPPADTPIRAITAISRLALLTLQGKGMVGLPGV
                     AARLFSTIAQEQINVLMISHSSSEYNICVLIEEAYAAQAQAAIERQFALEQSQGLIEG
                     QTVRGGVSIVAVIGAGMRGKPGVTGQIFTALAEAGVEVLAIAQGSSELNLSFVVAGAK
                     EDAAVQAIHHYFGLDR"
     gene            1854..2843
                     /gene="thrB"
                     /locus_tag="VF276_09340"
     CDS             1854..2843
                     /gene="thrB"
                     /locus_tag="VF276_09340"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX6607102.1"
                     /translation="MSGPDCTRARAVRVRVPATAANLGPGFDVLGLALTLYNEFTVRE
                     TGGRLHITVAGADGLPDDESNLFYRAFATLCREAGRPVPGLRIHMALRIPSGRGLGSS
                     ATAVAGGLQAANALLGLDLSPEELLPLAVRLEHGHHPDNVAPALLGGLVANTCAGDTI
                     HSVRLPFPDDMLAVVFIPDFEMDTVRGRKLMPAHYSQADVVFNTSRVALFLAALTSRR
                     YDLLGAAMEDRLHQPYRTQLFPALPALIAAARGAGAWGACLSGGGSTVLALTPRGPVA
                     EQIGAAMAQAAEAAGGIGGEVCVLEIERQGATAAALEMEQQGATASVAGEDKL"
     gene            2840..4126
                     /gene="thrC"
                     /locus_tag="VF276_09345"
     CDS             2840..4126
                     /gene="thrC"
                     /locus_tag="VF276_09345"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX6607103.1"
                     /translation="MSAMLACAVCGRQYHRDEVRYRCDCGETLEVAFAPDDPVRQGAT
                     KALFDARRAARRGPGASGTWRFRELLPAFGDDEIVSKLEGNTNLYPVGATERGGPGRL
                     GAYAGLDTLYLKHEGENPTGSFKDRGMTVGASQARRLGARAVACASTGNTSASLAAYA
                     AQAGIPCFVFIPAGAVAVGKLAQTLGYGAITVQVQGDFDDAMRLVQEVSADLGIYLLN
                     SINPFRIEGQKTIGWELLQDLDWQAPDWIVLPAGNLGNTAALGKALAEAQALGLIDRV
                     PRVAAIQASGAAPFYQSYQSGFAAPVRVHAETVATAIKIGNPVSYRRAAAVVRATEGV
                     VAAVDDAAILDAKAQIDRAGIGCEPASAATLAGVRQLVAAGVIAPQAVVAGILTGHML
                     KDPDTTIRAAAAAQREPLSVAADRRAVATLLAPYLR"
BASE COUNT          710 a         1552 c         1466 g          659 t
ORIGIN      
        1 ccttcgccgt aggtaccccg gccagcaaag ccatccaaat aaactacagt cttccaacga
       61 cttcctaaga ttttggaggc tgatgctaga tacatgtcca gtatctggtg cttacgcttg
      121 gaccaatctt tgacttcgct gaagaactcc ctgctggtag actgagacat aggggactcc
      181 actagcagat aaggccatat gccaagctga ttattgttaa caatcctggg tgctatcaca
      241 ttatacatga aaagcatttc ctttacagac tttgaagcgc accacccgcc ttgacactcc
      301 ggcgcccggc ggatagaatg agagcatcca ggcggcgttc gacccgatgc cgagtccgcc
      361 cctgtggcgc aatgaagatc cgtggcagtg ttcgcgccca gcgccgtccg tgtgacccgc
      421 gctgggcgtg ttgtgtctag caagttgaag ggccgtatgc ttgtaatgaa atttggcggc
      481 acgtcggtgg gcagcgccgg gcgcatccgc gaggtggccg ggctggtgca gcgcaccgcc
      541 gccgaggaac cggtggtcgt ggtggtgtcc gcagtcagcg gcgtaaccga tagcctgatc
      601 gccgcagcgc ggcaggcggc ggagggccgg cggttgcaag ccatccagca cgccatcggc
      661 atcgagcagc gccataccga gatcctgaac gcgctggacc tgcccgccga ggagcggcag
      721 agcatggcga ccgaactgcg cgccctcagc ggcgacctgg agaacttcat ccacagcgtc
      781 ggcacgctgg gcgagctcac gcagcgcacg ctcgactaca tcagcagcat gggcgagcgc
      841 atgagctgcc gcctgctggc ggcgaccctg cgcgccgcgg gccagcccgc cgaggccgtg
      901 gatgccacca aggtcgtcgt caccgacaac aacttcggcc atgccttccc gctgatggac
      961 ccaacgcggg cgcaggccga agccgtgctg gccccgcgcc tgcgcgaggg ccgcgtgccc
     1021 gtcgtcaccg gcttcatcgg cgccaccgag gccggcatcg tgaccacgct gggccgcggc
     1081 ggctcggact actccgccgc catcctgggc agcgcgctcg gcgcccgcga ggtggtgatc
     1141 tggaccgacg tggacggcgt gatgacggcc aacccgcgcg tggtgcccgg cgcccgcacg
     1201 ctgccgcgca tgtcgtacct ggaagccgcc gagatgtcgt atttcggggc caaggtgctg
     1261 caccccaaga ccatcgcccc ggcggtcgag ggcggcatcc ccgtgcgtat caagaacacc
     1321 ttcgcgcccg acgcgcccgg cacattgatc actgccgagg ccccgcccgc cgacacgccc
     1381 atccgcgcca tcaccgccat ctcgcgcctg gcgctgctga ccttgcaggg caagggcatg
     1441 gtcggcctgc ccggcgtggc cgcccggctc ttctcgacca tcgcccagga acagatcaac
     1501 gtgctgatga tctcgcattc gtcgtcggag tacaacatct gcgtgctgat cgaggaagcc
     1561 tacgccgccc aggcccaggc ggccatcgag cggcagttcg cgctcgaaca gagccagggc
     1621 ttgatcgagg gtcagaccgt gcgcggcggc gtctcgattg tcgccgtgat tggcgcgggg
     1681 atgcgcggca agccgggcgt caccgggcag atcttcaccg cgctggccga ggccggcgtc
     1741 gaggtgctgg cgattgccca gggatcgtcg gagttgaacc tgtcgtttgt cgtcgccggc
     1801 gccaaggagg atgccgccgt gcaggcgatc caccactact tcgggttaga ccgatgagcg
     1861 ggcctgactg tacccgcgcc cgcgccgtgc gcgtccgcgt gcccgccacg gcggccaacc
     1921 tcggccccgg cttcgacgtg ctcggcctgg cgctgacgct ctacaacgag ttcaccgtgc
     1981 gcgagacagg cgggcgcctg cacatcaccg tcgccggggc ggacggcctg cccgacgacg
     2041 agtcgaacct gttctatcgc gccttcgcca cgctctgccg cgaggcggga cggcccgtgc
     2101 ccggcctgcg catccacatg gccctgcgca tccccagcgg gcgcggcctg ggcagcagcg
     2161 ccaccgccgt cgccgggggg ctacaggcgg cgaacgccct gctcgggctc gacctgtcgc
     2221 cggaggaact gctgccgctg gcggtgcgcc tggaacacgg ccaccacccg gacaacgtcg
     2281 cgcccgcgct gctcggcggc ctggtcgcca acacctgcgc cggcgacacc atccacagcg
     2341 tgcgcctgcc cttccccgac gacatgctgg ccgtggtctt catccccgac ttcgagatgg
     2401 acacggtgcg cgggcgcaag ctcatgcccg cgcactactc gcaggccgat gtggtgttca
     2461 acaccagccg tgtggccctg ttcctggcgg cgctgaccag ccgacgctat gacctgctcg
     2521 gcgcggccat ggaagaccgc ctgcaccagc cctaccgcac gcaactcttc cccgccctgc
     2581 ccgcgctcat tgcggcggca cggggcgccg gcgcctgggg cgcctgcctg tcgggcggtg
     2641 gctcgacggt gctggcgctg acgccgcgcg ggccggtcgc cgagcagatc ggcgcggcca
     2701 tggcccaggc cgccgaggca gcgggcggca tcggcggcga ggtctgcgtg ctggagatcg
     2761 agcggcaggg cgccaccgcc gccgcgctgg agatggagca gcagggcgcc accgccagcg
     2821 tggccgggga ggacaagcta tgagcgcaat gctggcctgc gccgtctgcg ggcggcaata
     2881 ccatcgcgac gaggtgcgct accgctgcga ctgcggcgaa acgctggaag tcgccttcgc
     2941 gccggacgat cccgtccgcc agggagcgac aaaggcacta ttcgatgcgc gacgcgccgc
     3001 ccgccgcggg ccgggcgcca gcggcacctg gcgcttccgc gagttgctgc ctgccttcgg
     3061 ggacgacgag atcgtcagca agctcgaagg caacaccaac ctctacccgg tgggcgcgac
     3121 cgagcgcggc ggccccggtc gcctgggcgc gtatgcgggc ctcgacacgc tctacctgaa
     3181 gcatgagggc gagaacccca ccggcagctt caaggatcgc ggcatgaccg tgggcgccag
     3241 ccaggcccgc cgcctggggg cgcgcgccgt ggcctgcgcc tccaccggca acacctccgc
     3301 gtcgctggcc gcctacgccg cgcaggcggg catcccctgc ttcgtcttca tccccgccgg
     3361 ggccgtggcc gtgggcaagc tggcccagac gctcggctac ggcgccatca ccgtgcaggt
     3421 gcagggcgac ttcgacgacg ccatgcgcct ggtgcaggaa gtcagcgccg acctgggcat
     3481 ctacctgctc aactcgatca accccttccg catcgaaggg cagaagacca tcggctggga
     3541 actgttgcag gacctggatt ggcaggcgcc cgactggatc gtgctgcccg cgggcaacct
     3601 gggcaacacc gccgcgctcg gcaaggccct ggccgaagcc caggccctgg gtctgatcga
     3661 ccgggtgccg cgcgtggccg ccatccaggc cagcggcgcc gcgcccttct accagagcta
     3721 ccagagcggc ttcgccgccc cggtccgtgt ccacgccgaa acggtcgcca cggcgatcaa
     3781 gatcggcaac ccggtgagct accgccgcgc cgccgccgtc gtgcgcgcca ccgagggcgt
     3841 ggttgctgct gtcgatgatg cggcgatcct cgacgccaag gcgcagatcg accgcgcggg
     3901 catcggctgc gaaccggcca gcgccgccac cctggccggc gtgcgccaac tggtggccgc
     3961 cggggtgatc gcgccgcagg ccgtcgtcgc gggcatcctg accggccaca tgctgaagga
     4021 cccggacacc acgatccgcg cggcggcagc ggcccagcgg gaaccgctgt ccgtcgccgc
     4081 cgaccgccgg gcggtcgcga cgctgctggc cccctatttg cggtaggcat tacggagcca
     4141 cctgttggca tcctatatcg gcccgaagcg ccgggcgcgc agagcgtatt accactacat
     4201 tctcgttata attatggaga aggcattaca ctcggcgtta ttcgtgaaac gttgacacta
     4261 gcgacgagga tcaaggcatg catccactct tccgagagat attggagata attctagcgc
     4321 aacggcagga atccggagga ttagtattga ttccggagcc tgaagaggat gaagaaggca
     4381 tttattt
//
LOCUS       DASUYC010000570         2222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1000667, whole genome shotgun sequence.
ACCESSION   DASUYC010000570 DASUYC010000000
VERSION     DASUYC010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000667"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VF276_09350"
     CDS             <1..440
                     /locus_tag="VF276_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003355316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="octanoyltransferase"
                     /protein_id="HEX6607104.1"
                     /translation="RNGRPNPGHTDPAGAVCFDEATDYEIVVEGRKVIGSAQTRRGGA
                     LLQQGSVLLDADLERWSRAFRHPSEEARVRAQTHLTLRMAGLNDFAPAPLAPEAVTAA
                     LATGLMEALQIMLEPGTLTADEAARWADLVATKYATDAWTLRK"
     gene            619..2088
                     /gene="lpdA"
                     /locus_tag="VF276_09355"
     CDS             619..2088
                     /gene="lpdA"
                     /locus_tag="VF276_09355"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX6607105.1"
                     /translation="MFTGVDEMYEYDIAVIGGGPGGYVAALDAGMRGANVLLIEKDRR
                     LGGTCLLRGCIPSKTMVKTAEILAEMRHAADFGVTGTAEPNWEGIVARKDKVVDGLTR
                     GINDLFKARKITRLEGKARLLTPGSIYVQGANQYAGAALTVRAKNIIVATGSATVLGS
                     EVPGGDSPRVIHSDQILDMDLTLRAIPTLPKSMVVIGGGIVGMEFASIFAAYGTKVTA
                     VITSPRILRNVDEEIARRFSALLKQAGVETLVKTSVQEFRDRGDHLEVVVQTEGSAEP
                     RVLQCEYALYAKGRVPYTDGLGLDDLGVQTQRGYVTVDDHLRTNVPNVYAIGDVVPGH
                     MLAHVASYEGEVAVDNILGHERAADYRAVPDCIFTIPEIASVGMTEAVAKERGVPVRV
                     TKFPFAALGRAQAIGEPHGLVKLVCHAEDRTVLGMQILGAHASDVIAEGTLAVQQRVT
                     AEDLAHTMHAHPTMPEAVQETAIAQIFGTPLHVNMFTRG"
BASE COUNT          406 a          740 c          746 g          330 t
ORIGIN      
        1 ggcgcaacgg ccggcccaac cccggccaca ccgacccggc gggcgccgtc tgcttcgacg
       61 aggccaccga ctacgagatc gtggtcgagg gccgcaaggt catcggcagc gcacagaccc
      121 ggcgcggcgg ggcgttgctg cagcaagggt ccgtgctgct ggatgccgac ctggaacgct
      181 ggtcgcgggc tttccgccac cccagcgagg aggcccgcgt gcgggcgcaa acccacctca
      241 ccctgcgcat ggccggcctc aacgacttcg cgcccgctcc gctggcgccc gaagccgtga
      301 cggcggccct ggcaacgggg ttgatggagg cgttgcagat catgctggag cccggcacgc
      361 tcaccgcgga cgaggccgcc cgctgggccg atctcgtcgc cacgaaatac gcgacggatg
      421 cctggacgct gcggaagtag cgatcagcta tcagccgtca gctatcagga tagaggcgtt
      481 tcagatagct tttggactga cgggaaccga tgtatgacgg atagcacggt ctatattacg
      541 gaggctgaca accaccaact gacggctgac ggctgaaacc tgacggctga tagctgacag
      601 ctataaggaa ccactttgat gtttactgga gtcgacgaaa tgtacgaata cgatattgcg
      661 gtgattggcg gcgggcccgg cggctatgtg gccgcgctcg acgcggggat gcgcggcgcc
      721 aatgtgctgc ttatcgaaaa ggaccgccgc ctgggcggca cctgcctgtt gcgcgggtgc
      781 atcccgtcca agacgatggt caagaccgcc gagatcctcg cggaaatgcg ccacgccgcc
      841 gatttcggcg tcaccggcac ggcggaaccc aactgggaag gcatcgtggc ccgcaaggac
      901 aaggtggtgg acggcctcac gcggggcatc aacgacctgt tcaaagcgcg caagatcacc
      961 cgcctcgaag gtaaggcccg cctgctgacg ccgggcagca tctacgtgca gggggcgaac
     1021 cagtacgcgg gcgcggcgct caccgtgcgc gccaagaaca tcatcgtggc gaccgggtcg
     1081 gccaccgtgc tgggcagcga agtgccgggc ggcgactcgc cgcgcgtgat ccacagcgac
     1141 cagatcctcg acatggacct gaccctgcgc gccatcccca ccctgcccaa gagcatggtc
     1201 gtaatcggcg gcggcatcgt cggcatggag ttcgcctcga tcttcgcggc ctacggcacc
     1261 aaggtcacgg cggtgatcac ctcgccgcgc atcctgcgca acgtggacga ggagatcgcg
     1321 cggcgcttct cggcgctgct caagcaggcg ggggtcgaaa cgctggtcaa gacgagtgtg
     1381 caggagttcc gcgaccgggg cgaccacctg gaggtggtgg tgcagaccga gggtagcgcc
     1441 gagccgcgcg tgttgcagtg cgaatacgcg ctctacgcca agggccgcgt cccctacaca
     1501 gacgggctgg gcctcgacga cctgggcgtg cagacgcagc gcggctatgt caccgtggac
     1561 gaccacctgc gcaccaacgt gcccaacgtc tacgccatcg gcgacgtggt gccggggcat
     1621 atgctcgccc atgtggcgtc ctacgagggc gaggtggccg tggacaacat cctgggccac
     1681 gagcgtgccg ccgactaccg cgccgtgccc gactgcatct tcaccatccc cgaaatcgcc
     1741 agcgtgggca tgaccgaggc ggtcgccaaa gagcgcgggg tgccggtgcg cgtgaccaag
     1801 ttcccgttcg ccgcgctggg ccgcgcccag gctatcggcg agccgcacgg cctggtcaag
     1861 ctcgtctgcc acgccgagga ccgcacggtg ctgggtatgc agatcctggg cgcccacgcc
     1921 agcgacgtga tcgccgaggg cacgctggcc gtccagcagc gcgtcacggc ggaagacctg
     1981 gcccacacca tgcacgccca cccgacgatg ccggaagcgg tgcaggaaac ggccatcgcc
     2041 cagatcttcg gcacgccgct gcacgtcaat atgttcaccc gcggctaggc cgcgcatttc
     2101 aacgcagaca actacggagg ggcactgcaa ggtgcccctc tgttatttgg cggactcatt
     2161 accgcttcca gtgagacttg tcactctgag cggagcgaag agtctcgtct tccagtgaga
     2221 ct
//
LOCUS       DASUYC010000571         5878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1055613, whole genome shotgun sequence.
ACCESSION   DASUYC010000571 DASUYC010000000
VERSION     DASUYC010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5878
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055613"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="VF276_09360"
     CDS             complement(<1..679)
                     /locus_tag="VF276_09360"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX6607106.1"
                     /translation="MKRKVAVLHTSTVFVNDDPILRDLFAEILPEVELLEFVDTDLLA
                     TVTSEGCITPAAIRRIMHLAQAAEQAGADVILSACSAVGPAIPTARRAVSIPIVPIDE
                     PMARRAVQTAEEIGVLATLGMALAPMVDLLEQQASIVRRPVCIHRMLVPDAFDALEAG
                     RQEVHDALVVEAARDLATVADVIVPAQVGLARLVPRLHTATGRKVFHGPRLAVQHIKQ
                     LLAELPPK"
     gene            1023..2426
                     /locus_tag="VF276_09365"
     CDS             1023..2426
                     /locus_tag="VF276_09365"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX6607107.1"
                     /translation="MFGHLTSSPRSRRVAPIRRVRTGITALSVCALLSLLLAACGGEA
                     PATSPPVSAPTTAPAAEATATTAPAAAEPTATTAAGGSGGATASGGTILFGAPLGLTG
                     SLTKESKLTQQGYELWKEAVNAAGGIKVGDKTYMVETKYYDDESKQDKSAQLAEKLIS
                     EDKVNFLLGPYGTSATQSTAAIAEKHEIPMVEGEGAAVKIFSQGYKYTFGTLSPAPNY
                     LRGVIDMAAAQNPKPQKVVILSANDAFSVEVADGAKQAAEEKGMTVAAYIKYPNAETN
                     LTAQVTQAKGQSPDLILNSGHFAEAVAIVKAMKELGVSPMGAGFSVGPSLPDFKDTLK
                     ADADYVYGGSQWTKDLKYNGDDYWKTPQAYFDAFVKRWGNEPSYQSAMATACGLVFQK
                     AIEKAGTLDPKAVRDAIAATDFVSFAGQMKFDERGINTVKPMVVEQWQNGEKQTVWPA
                     EAASGKPQWPAPAWDKR"
     gene            2758..3636
                     /locus_tag="VF276_09370"
     CDS             2758..3636
                     /locus_tag="VF276_09370"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX6607108.1"
                     /translation="MASQVVINGLLLGAMLGLVGLGFSLVWGILNIVNLAHSAFIMFG
                     AYVTFVLFAQLNLDPFVSLPVSMAALFLLGFVLQKYVINLVMRAPLLVTFVLTFGFET
                     FFTSLARAFFTGDQRAVSPAYNAAAITLGDAILNWMKLGAFVVALVLTGALYLFMRYT
                     RTGNAIRAVGMDADAARLMGVDVARIYALTYGLSAAVAGAAGTLLSMWYSFTPDSFGI
                     YNIRAFAVVVLGGLGSIPGALIGGLVFGLLDQAISTGSIGDFQLGALKEALIFLAMVV
                     ILVVRPTGLLGKEGYR"
     gene            3636..4715
                     /locus_tag="VF276_09375"
     CDS             3636..4715
                     /locus_tag="VF276_09375"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX6607109.1"
                     /translation="MNLLIMLAALVLSIMVVQHRHPALNRVGLIGLVALGLLAPFISN
                     NLATLTGVYVAGTAAIGWNIIGGFTGYAAFGQSAFFGLGGYTLAVMIGRAQGTFGLPG
                     WLGVLVAGVVPAVAAVLIGLVVLRLRGHYFAIATLGVGIAVRELVNNIDCVGLPGSAH
                     PLFCLGAASGIILPPVRKDDLRATDLALYFAAFALIAGGTAALWLLNRSKFGYGLRAI
                     RANEEAASVMGINITTFKIAAFTAGAALTGFAGGVQAIVNGSVLPDQTSIFDPYRSLE
                     VIIICLIGGAGTVWGPVVGTFVLYALQEALNGVFGAGDWRPIFFGAIVILLVLFLPRG
                     ILQFFGARQALGWRVLWRNLVANRI"
     gene            4868..5677
                     /locus_tag="VF276_09380"
     CDS             4868..5677
                     /locus_tag="VF276_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6607110.1"
                     /translation="MTETPTPPAEPVLQVEGATKLYGGVAAVQDISFAVYPGEILGLI
                     GPNGAGKTTLVNLITGTTPASRGTIRFKGRSLRGLPPHVIGSQGIARTFQVVRPFQNL
                     TVLENVAVGAMYGRGGHNRTAAAAFAHAAEVLQVVDLSRQAHLLAESLPIAGRKRLEL
                     AKALAMDPELLLLDEAMAGLRGREIDEAMALIQRINRTGVTILVIEHVMKVIQGVCHR
                     VVVLDYGRKIAEGPPAAVMQDPAVIQAYLGQRYARTLAQQDGPGEGPGGDE"
     gene            5724..>5878
                     /locus_tag="VF276_09385"
     CDS             5724..>5878
                     /locus_tag="VF276_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012612710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6607111.1"
                     /translation="MLQIRKLNVAYGDVQVLWDVDLEVYPGEIVALVGANGAGKSTLL
                     ATISGLL"
BASE COUNT          916 a         2001 c         2028 g          933 t
ORIGIN      
        1 gcttgggcgg cagttcagcc agcagttgct tgatgtgctg cacagccagg cgcgggccgt
       61 ggaacacctt gcgccccgtg gcggtgtgca gccggggcac cagtcgggcc agccccacct
      121 gcgcaggcac gatcacgtcg gcgacggtcg ccaggtcacg tgccgcttcg accaccagcg
      181 cgtcatgcac ctcctgccgg cccgcctcca gagcgtcaaa ggcgtcgggc accagcatgc
      241 ggtggatgca gacgggccgg cgcacgatgc tcgcctgttg ctccagcaga tcgaccatcg
      301 gggccagcgc catgccgagc gtcgccagca cgccgatctc ctcggcggtt tgcacagcgc
      361 gccgcgccat cggttcgtcg atgggcacaa tggggatgga gacggcgcgg cgggcggtgg
      421 ggatcgccgg gccaacggcc gagcaggccg agaggatcac atcggcgccc gcctgttccg
      481 ctgcctgcgc caggtgcatg atgcgccgga tcgccgctgg ggtaatacag ccctcgctgg
      541 tgacggtggc gagcaggtcg gtgtcgacaa actccagcag ttcgacctcg ggtaggatct
      601 cggcgaatag atcgcgcaag atcgggtcgt cgttgacgaa cacggtgctg gtgtgcagca
      661 cggccacttt gcgcttcata gcggtccctc ctgtggtatc gcgccggggc gcgccggagc
      721 cggcgtgccc catttctact accattgtgc cacagggccc ccggcaggcc ggttataaag
      781 cgactacccg cgggcaagac tcggccacgc ccggctgggg tataccccgc aagtcgccgg
      841 ctcgtgttac gcaggccgcc cgcgcggcct gggctgaacg gcggaatctc gtgccgcgag
      901 ataatagggg cgaatttgcc cccattgacg gcccgactgt agtataataa gcccgcttct
      961 aaccggccca tgcccgcaac cggggcatct gttactcgac ctttcgccaa ggaggagaga
     1021 caatgtttgg gcaccttact tcatcccccc gcagccgccg ggtcgcgcct atccgccgcg
     1081 tgcgtaccgg cataaccgcg cttagcgtct gcgctctgct gagcctgctg ctggccgcgt
     1141 gcggcggcga ggccccggcg acatccccgc cggtcagcgc gccgaccacg gcccccgccg
     1201 ccgaggctac cgctacgacc gcgcccgccg ccgcggagcc gacggcgacc accgccgccg
     1261 gcggatcggg cggggcgacc gcgagtggcg gcactatcct gtttggcgcg ccgctcggcc
     1321 tgaccggctc gctcaccaaa gagtccaagc tgacccagca gggctatgaa ctctggaaag
     1381 aggccgtcaa cgccgcgggc ggcatcaagg tcggcgacaa aacctatatg gtcgagacca
     1441 agtattacga cgacgagagc aagcaggaca agagcgccca actggccgag aagttgatca
     1501 gcgaggacaa ggtgaacttc ctgctcggcc cctacggtac gtcggccacg cagtccaccg
     1561 cggccatcgc cgagaagcac gagatcccca tggtcgaagg cgagggcgcc gcggtgaaga
     1621 tcttcagcca gggctataag tacaccttcg gcacgctcag ccccgcgccc aactacctgc
     1681 gcggcgtgat cgacatggcc gccgcccaga accccaagcc gcagaaggtg gtgatcctct
     1741 cggccaacga cgccttctcg gtggaggtgg ccgacggcgc caagcaagcc gccgaggaaa
     1801 agggcatgac ggtcgcggcc tacattaaat acccgaacgc cgagaccaac ctgaccgccc
     1861 aggtcacaca ggccaagggc cagagccctg acctcatcct caacagcggc cacttcgccg
     1921 aagccgtcgc catcgtcaag gcgatgaagg aactgggcgt tagcccgatg ggcgcgggct
     1981 tcagcgtggg gccgtcgctg cccgacttca aggacacgct gaaggccgac gccgactacg
     2041 tctacggcgg ctcgcagtgg accaaggacc tgaagtacaa cggcgacgac tactggaaga
     2101 cgccgcaggc ctatttcgac gccttcgtga agcgctgggg caacgagccg tcgtaccaga
     2161 gcgccatggc gaccgcctgc gggctggtgt tccagaaggc gatcgagaaa gccggcacgc
     2221 tggaccccaa ggccgtgcgc gacgccatcg cggcgaccga cttcgtcagc ttcgccgggc
     2281 agatgaagtt cgacgagcgc ggcatcaaca ccgtcaagcc gatggtggtc gagcagtggc
     2341 agaacggcga gaagcagacg gtgtggccgg cagaggccgc cagcggcaag ccgcaatggc
     2401 ccgccccggc gtgggataag cggtagcccg gaggcgggta gcgcggaggc taaagcctcc
     2461 ggctaccctt acgaagcccg cctgcgcggg ctgggccata cggggatagg ccgcggaggc
     2521 ggcctttgta gatatagccc gcccgttgac gggcggggcg gggctatcgg tagcgcggag
     2581 gctaaagcct ccggctaccc ttacgaagcc cgcctgcgcg ggctatagcg tcaggccgcg
     2641 gaggcggcct ttgtagatgt agcccgcccc ttgaggggcc gggcggggcc tatcgatagc
     2701 ccgccctttg cgggacggga caactgggcc gggcggcgcg gcgggaagcg agggacgatg
     2761 gcgagtcaag tcgtgatcaa cggcctgctg ctcggcgcga tgctggggct ggtcgggctg
     2821 gggttctcgc tggtctgggg catcctgaac atcgtgaatc tggcgcactc cgccttcatc
     2881 atgttcgggg cttacgtgac ctttgtgctg ttcgcgcagc tcaacctcga cccgttcgtg
     2941 tcgctgccgg tcagcatggc cgcgctcttc ctgctgggct tcgtgctgca aaagtatgtc
     3001 atcaatctgg tgatgcgcgc gccgctgctg gtgacgttcg tgctgacctt cgggtttgag
     3061 acgttcttca ccagcctggc gcgcgccttc ttcaccggcg accagcgcgc cgtctccccg
     3121 gcctacaacg ccgccgccat cacgctgggc gacgccatcc tcaactggat gaagctgggc
     3181 gccttcgtcg tggccctggt gctgaccggg gcgctgtacc tgttcatgcg ctacacgcgc
     3241 acgggcaacg ccatccgcgc cgtgggcatg gacgccgacg ccgcccggct gatgggcgtg
     3301 gacgtggcgc gcatctacgc gctgacctac ggcctcagcg ccgccgtggc cggggcggcc
     3361 ggcacgctac tgtcgatgtg gtacagcttc acgcccgact cgttcggcat ctacaacatc
     3421 cgcgccttcg ccgtggtggt gctgggcggg ttgggcagca tccccggcgc gttgatcggc
     3481 gggctggtct tcggtctgct tgaccaggcc atctccaccg gcagcatcgg cgacttccag
     3541 ttgggggcgc tcaaggaggc gctgatcttc ctggcgatgg tcgttatcct cgtggtgcgc
     3601 ccgaccgggc tgctgggcaa ggagggctac cgctagtgaa cctgctgatc atgctcgccg
     3661 cgctcgtgct gagcatcatg gtggtccagc accgccaccc ggccctgaat cgcgtgggcc
     3721 tgatcggcct ggtggccctg ggcctgctgg cgccgttcat ctccaacaac ctggcgacgc
     3781 tgaccggcgt ctacgtggcc gggacggcgg ccatcggctg gaacatcatc ggcggcttca
     3841 ccggctacgc ggccttcggc cagtcggcct tcttcggcct gggcggctac acgctggcgg
     3901 tgatgatcgg gcgggcgcag ggcacgttcg ggctgcccgg ctggctcggc gtgctggtcg
     3961 cgggcgtggt cccggcggtg gccgccgtgc tgatcgggct ggtggtgctg cgcctgcgcg
     4021 gccactactt cgccatcgcc acgctgggcg tgggcatcgc cgtgcgcgaa ctggtcaaca
     4081 acatcgactg cgtgggcctg cccggcagcg cgcacccgct gttttgcctg ggcgcggcca
     4141 gcggcatcat cctgcccccg gtgcgcaagg acgatctgcg cgccaccgac ctggcgctct
     4201 acttcgccgc cttcgcgctc atcgccgggg gcaccgccgc gctctggctg ctcaaccgca
     4261 gcaagttcgg ctacggcctg cgcgcgattc gggccaacga ggaagcggcg agcgtgatgg
     4321 gcatcaacat caccaccttc aagatcgccg ccttcaccgc gggcgcggcg ctgaccggct
     4381 tcgccggggg cgtgcaggcc atcgtcaacg gctcggtgct gcccgaccag accagcatct
     4441 tcgaccccta ccgcagcctg gaggtgatca tcatctgcct catcggcggg gcgggcacgg
     4501 tctggggacc ggtcgtcggc accttcgtgc tctacgcctt gcaggaggcg ctcaacggcg
     4561 tcttcggcgc gggtgactgg cggcccatct tcttcggggc catcgtcatc ctgctcgtcc
     4621 tgttcctgcc gcgcggcatc ctgcaattct tcggcgcccg gcaagccctg ggctggcggg
     4681 tgctctggcg caacctcgtc gccaaccgga tctagccggt ccaacctgcg atgtaggggc
     4741 cggtttcaaa ccggcccgcg gcgccaacct gcgctgtagg ggccggtttc aaaccggccc
     4801 gcgatgccga tatgcgatgt aggggccggt ttgaaaccgg cccggcgccc tggcattagg
     4861 agattctgtg actgagacgc caacgccgcc cgccgagccc gtgttgcagg tcgagggcgc
     4921 gaccaagctc tatgggggcg tggccgccgt gcaggacatc agcttcgccg tctaccccgg
     4981 cgagatcctg ggcctcatcg ggccgaacgg cgcgggcaag accacgctgg tcaacctgat
     5041 caccgggacc acgccggcca gccggggcac catccgcttc aaggggcgct cgctgcgcgg
     5101 actgccgccg cacgtcatcg gcagccaggg catcgcgcgc accttccagg tggtgcgccc
     5161 gttccagaac ctcaccgtgc tggagaacgt ggccgtgggg gcgatgtacg ggcgcggcgg
     5221 ccacaaccgg accgccgccg ccgcctttgc ccacgccgcc gaggtcttgc aggtggtgga
     5281 cctgagccgc caggcccatc tcttggcgga atcgttgcct atcgccgggc gcaagcggct
     5341 cgaactggcg aaggccctgg cgatggatcc cgaactgctg ctgctcgacg aggccatggc
     5401 cgggctgcgc gggcgcgaga tcgacgaggc catggccctg atccagcgca tcaaccgcac
     5461 cggcgtgacg attctcgtca tcgagcatgt gatgaaggtg atccagggcg tctgccaccg
     5521 cgtggttgtg ctcgactatg gccgcaagat cgccgagggg ccgcccgccg ccgtgatgca
     5581 ggacccggcg gtgatccagg cgtatctcgg ccagcgctac gcgcggacgc tggcgcagca
     5641 ggacgggccg ggggagggac caggcggtga cgagtaacgc gccggagttg ggccgccgcc
     5701 gggcgcccgg cggcgccgcg ccgctgctcc agatccgcaa gctcaacgtg gcctacggcg
     5761 atgtgcaggt gctgtgggac gtggacctgg aggtttatcc cggcgagatc gtggccctgg
     5821 tgggcgcgaa cggcgcgggc aaaagcacgc tgctggcgac gatcagcggc ctgctgat
//
LOCUS       DASUYC010000572         2835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1419355, whole genome shotgun sequence.
ACCESSION   DASUYC010000572 DASUYC010000000
VERSION     DASUYC010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1419355"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2775)
                     /locus_tag="VF276_09390"
     CDS             complement(<1..2775)
                     /locus_tag="VF276_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607112.1"
                     /translation="MPMPISLPRTRRPRVALAGIVLALCALLVVAGTPAPTQGASGQR
                     SPSLARGSRIPLADGSYFLSGANYPQFRYYGGDIATLAAVDGDCRWYYSSAFDYNLID
                     RDFAGMQAQGVHTVRWWLFGDGRGAPEFDYNGQVTGFDATFFDHMDQAMEIARRHNIY
                     VIWTLWDFLAFAPPNWLCGGATLHQQQAVAERLPADLRDPFLAHLKMAQQAPKEFLPG
                     KNAGDQPDAGQSCMVFAGGHRNLVTDTAPGGAQDSFFNNALIPMLQRYKNDPNIAGWE
                     IMNEPEWALIENPYTGQNPTMQQPVEVGQMQAFFRRFTQAVHTYAPDQYATVGAASLK
                     FMGFGPNLPPGIWNGLGFDYYNVHYYGWMDSPYNNGNPLTIDYNATAAQLDAPAIIGE
                     FPAHGGTAPIYLPSIRNTGTETTGLSLRYICTAYAPASEPPCTRSYTATIDYYSANGA
                     PAGTQTVTVPAYGEWAGPVAVGAGFSGTARIVSNGPLAAAVTQRGIPNGGEQTVYTGD
                     DQASARVLWLPLVSNGGAARTRIAVQNADIATNYVTVYYSNQAGQNVATDIANLAPRA
                     SVLIDPQLGGPLAGPPAGFQGSAVIIGSLPLVAVAYQLDPTQGSDAYNAEQVDDGTEV
                     YLPSVRNWGANGNPTIFVQNPWSGSSQITIRYYTAGGAQAGNAQTLTLPGNGSAAVQP
                     QAAGLPGGFEGSAVLSSTGRVAAVVRSVAGSSTVLYAGTRLTDQRLHFPVVHSPDSSG
                     NGPITSLSAQNVNATQPITLWVTVNDGAGGTPYFSDAVTVPPLGEWSATVRDLPGVPR
                     NFTGTAEILWPWANGPGGTWTDGFPLLAVARDLDGAHTSGSNYRGVASHSFAWAVTQA
                     TPRQLLEGIYANHWAGALAWSYYDNGTGSWRDYADASAAFDAAHAADLAIGHTVYTPI
                     PTPANDATATA"
BASE COUNT          388 a          883 c         1097 g          467 t
ORIGIN      
        1 cgccgtggca gtggcgtcgt tggctggggt ggggatcggc gtgtagacgg tgtggccgat
       61 agccaggtcg gcggcgtggg cggcgtcgaa ggcggcggag gcatcggcgt agtcgcgcca
      121 gcttccggtg ccgttgtcgt agtagctcca ggccagcgcc ccggcccaat gattggcgta
      181 gatgccttcg agcaactggc gcggggtggc ttgcgtgacc gcccaggcga agctatgcga
      241 ggccacgccg cggtagttcg acccgctggt gtgcgcgccg tccaggtcgc gggccacggc
      301 cagcagtggg aagccgtcgg tccaggtgcc gcccggcccg ttggcccagg gccacaggat
      361 ctccgccgtg ccggtgaagt tgcgcggcac gccgggcaga tcgcgcaccg tcgccgacca
      421 ctcgcccagg ggcggcacgg tcacggcgtc ggagaaatag ggggtgccgc ccgcgccgtc
      481 gttcaccgtc acccagaggg tgatgggctg ggtcgcgttc acgttttgcg cgctgagcga
      541 ggtgatcggg ccgttgccgc tgctgtcggg gctgtggacc accgggaagt gcaggcgctg
      601 gtcggtgagc cgcgtgccgg cgtagagcac cgtgctgctg cccgccacgc tgcgcaccac
      661 ggcggccacg cgcccggtcg aactgagcac ggcgctgccc tcgaagccgc ccggcagccc
      721 ggcggcctgc ggttggacag cagcggaccc gttgcccggc agcgtcagcg tctgggcatt
      781 gcccgcctgg gcgccgccgg cagtgtaata gcggatcgtg atctgggaag atccggacca
      841 cgggttttgc acgaagatcg tcgggttgcc gttcgcaccc cagttgcgca ccgagggcag
      901 atagacctcc gtcccgtcgt ccacctgctc ggcgttgtag gcgtcgctgc cctgcgtcgg
      961 gtcaagttgg taggccacgg ccacgagcgg caggctgccg atgatcacgg cgctgccctg
     1021 gaacccggcg ggcggcccgg cgaggggtcc gcccagttgc gggtcgatca gcaccgaggc
     1081 gcgcggcgcc aggttcgcga tgtcggtggc gacgttttgt cccgcctggt tggagtagta
     1141 cacggtgacg tagttggtgg cgatgtcggc gttttgcacg gcgatgcgcg tgcgcgccgc
     1201 tccgccgttg ctcaccagcg gcagccacag cacccgcgca ctggcctggt cgtcgccggt
     1261 atagacggtc tgctcgccgc cgttggggat gccgcgctgg gtgacggcgg cggccagcgg
     1321 gccgttggac acgatacgcg ccgtgccgct gaagcccgcc ccgaccgcga ccgggccggc
     1381 ccactcgccg taggcgggca ccgtgactgt ctgcgtgcct gccggtgccc cgttcgcgct
     1441 gtaatagtcg atggtggccg tatacgagcg cgtgcagggt ggttcggaag ccggggcgta
     1501 ggcggtgcag atgtagcgca ggctcaggcc ggtcgtttcg gtgccggtgt tgcggatgct
     1561 gggcaggtag atgggcgccg tgccgccgtg ggccgggaat tcgccgatga tcgccggggc
     1621 gtcgagttgg gccgccgtgg cgttatagtc gatggtgagc gggttgccgt tgttgtaggg
     1681 gctgtccatc cagccgtaat agtggacatt gtagtaatcg aagcccagcc cgttccagat
     1741 gccgggcggc aggttggggc cgaagcccat aaacttgagc gaagccgcgc ccaccgtggc
     1801 gtactggtcc ggcgcgtagg tatgcaccgc ctgggtgaag cggcggaaaa acgcctgcat
     1861 ctggccgact tccaccggtt gctgcatcgt ggggttctgc ccggtgtagg ggttctcgat
     1921 cagcgcccac tcgggctcgt tcatgatctc ccacccggcg atgttcgggt cgttcttgta
     1981 gcgctgcagc atcgggatga gggcgttgtt gaagaacgaa tcctgcgcgc cgccgggcgc
     2041 cgtgtcggtg acgaggttgc ggtggccgcc cgcgaagacc atgcaactct ggcccgcgtc
     2101 cggctggtcg cccgcgttct tgccgggcag gaactccttg ggcgcctgct gcgccatctt
     2161 caggtgggcc aggaagggat cgcgcaggtc ggcgggcagg cgctcggcca cggcctgctg
     2221 ctggtgtagc gtggccccgc cgcagagcca gttgggcggt gcgaaggcca ggaagtccca
     2281 cagcgtccag atcacgtaga tgttgtggcg gcgggcgatc tccatcgcct ggtccatgtg
     2341 gtcgaagaag gtggcgtcga agcccgtgac ctggccgttg tagtcgaact cgggcgcgcc
     2401 gcgtccgtcg ccgaagagcc accagcgcac cgtgtggacg ccctgggcct gcatgccggc
     2461 gaagtcccgg tcgatcaggt tgtagtcgaa ggcgctgctg tagtaccacc ggcagtcgcc
     2521 atccacggcg gccagggtgg cgatgtcgcc gccgtagtag cggaactgcg gatagttggc
     2581 gccggagagg aaatagctgc cgtcggccag agggatgcgg ctgccgcggg cgagactcgg
     2641 gctgcgctgg ccggatgccc cctgcgtggg ggcaggggtg ccggcgacga cgagcagcgc
     2701 gcacagcgcc aggacgatgc cggccagggc gacgcggggc cgccgggtgc ggggtagcga
     2761 gatgggcatg ggcatgggca gcactccttg aactgaagag cagtcaggac gcggggcgta
     2821 gcacgcgcag cggga
//
LOCUS       DASUYC010000573         2272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_913044,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000573 DASUYC010000000
VERSION     DASUYC010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913044"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..414
                     /locus_tag="VF276_09395"
     CDS             37..414
                     /locus_tag="VF276_09395"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX6607113.1"
                     /translation="MQVRTKIWIEQDGAVMLSDWRVALLEAVDATGSLAEAARQMDVP
                     YRTAWQKLKALEDRWGLALLDTASGGADGGASRLTPQAQELVHRFRQITAGVQEEAAA
                     RFAAAFRDIPGVLNVSSDDPLPD"
     gene            542..1468
                     /locus_tag="VF276_09400"
     CDS             542..1468
                     /locus_tag="VF276_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX6607114.1"
                     /translation="MRQCHTRLLLAAMLLLSLLLTACGGPAASSVGGTPSPVLAASVA
                     HAAAVTPPARPELILATTTSTQDSGLLDVLIPDFEARTGYQVKPVAVGSGAALKMGED
                     GNADVLLVHSPAAEEAFMAKDAGVDRRLIMHNDFIIVGPPGDPAHIKGQAALPAMKAI
                     AATGAPFVSRGDASGTNALELSLWKQAGIDPKGKPWYIVSGQGMGATLNVTDEKNAYT
                     ISDRATYLAFKARLQLQILAEKDRVLLNIYHVIRVNPAKQPSVNTAGAQAFADYLISP
                     EGQALIANFGTQKYGQALFVPDAGKDEASLGQ"
     gene            1478..2269
                     /locus_tag="VF276_09405"
     CDS             1478..2269
                     /locus_tag="VF276_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012370415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX6607115.1"
                     /translation="MQTGDEIPLAGGNVTPVVRVGETVRRVPGPWSPAVHRLLDHLAA
                     RGFDGAPRFLGLDAQGREILSYITGEVGQYPLPAYMWSDAALAGAARLLRRYHDATID
                     FVAPPGAEWQMVYPDAAQHEVICHNDFAPYNVVYVAEQPHAIIDFDLTGPGPRLWDLA
                     YAAYRFVPLSYAADMQELGLSDPTQQSRRLQLFCTAYGLAAPPGLLHMVAQRIEAMCD
                     LLASRAAAGDPAFRKMVDEGHLAYYQRELAAFHAWQPTLACLLGW"
BASE COUNT          363 a          788 c          755 g          366 t
ORIGIN      
        1 gcctgggtcg tcgtcatcca ccaggaggag tgagccatgc aggtgcggac caagatctgg
       61 atcgagcaag acggcgcggt gatgctgtcc gactggcgag tggctctgct cgaagcggtg
      121 gatgcgaccg gttcgctggc cgaggccgcc cggcagatgg atgtgcccta tcgcaccgcc
      181 tggcagaagc tgaaggcgct ggaagaccgc tggggcctgg ccctgctgga caccgccagc
      241 ggcggcgcgg acggcggcgc cagccggctc acgccccagg cccaggaact ggtgcaccgc
      301 ttccggcaga ttaccgccgg ggtgcaagag gaggccgccg ctcgcttcgc cgccgccttc
      361 cgtgacatcc ccggcgtgct caacgtttca tccgacgatc ccttaccgga ttgagcatca
      421 tctatcggca gcttgtgccg cggctgcctt cccaccacga accgaatcgc tcgacaggtc
      481 tttttaacgc ccgcgttatg caactatttg cataacgtcc cattgctacc agggagaacc
      541 catgcgccag tgccatacac ggctcctgct tgccgccatg ctcctgctta gcctgctgct
      601 gacggcctgt ggcggcccgg ctgcttcgtc cgtgggtggg actccgtccc cggtgctggc
      661 ggcgtccgtc gcccatgcgg cagcggtgac gcccccggcg cggcccgaac tgatcctggc
      721 gacgaccacc agcacccagg acagcggcct gctcgacgtg ctgatccccg acttcgaggc
      781 ccgcaccggc taccaggtga agccggtggc ggtcggctcc ggcgccgcgc tgaagatggg
      841 cgaggatggc aacgccgatg tattgctggt gcactcgccc gccgccgagg aagcgttcat
      901 ggcgaaggac gcgggcgtgg accgccgcct gatcatgcac aacgacttca tcatcgtcgg
      961 gccgcccggc gatcccgcgc atatcaaggg gcaggccgcg ctgcccgcga tgaaagccat
     1021 tgccgccacg ggtgcgcctt tcgtgtcgcg cggcgacgcc tccgggacga acgcgctgga
     1081 attgagcctg tggaagcagg ccgggatcga ccccaagggc aagccatggt atattgtctc
     1141 cgggcagggt atgggcgcca cgctcaatgt caccgacgag aagaacgctt acacgatcag
     1201 cgaccgcgcg acttacctcg cgttcaaggc gcggttgcag ttgcagatcc tggccgagaa
     1261 ggaccgcgtg ctgctcaaca tctaccacgt gatccgcgtc aacccggcga agcagccgtc
     1321 cgtgaacacg gcgggcgcgc aggctttcgc cgattacctg atctcgccgg aggggcaggc
     1381 gctgatcgcc aacttcggta ctcagaagta cgggcaggcg ctgttcgtgc ccgacgcggg
     1441 caaggacgag gccagtctgg gccagtagga gggccgggtg caaacaggcg acgagatacc
     1501 gctggccggc ggcaacgtga cgccggtggt gcgcgtgggc gagaccgtgc gccgcgtccc
     1561 cggtccgtgg agtccggcgg tgcatcgcct gctggaccac ctggccgcac ggggcttcga
     1621 cggtgcgccc cgctttctcg gcctcgacgc ccaggggcgg gaaatcctga gctacatcac
     1681 cggggaggtg ggccagtacc cgctacccgc ctatatgtgg tccgacgcgg cgctggcggg
     1741 cgctgcccgg ctgctgcgcc gctaccatga cgcgacgatt gacttcgtgg ccccgcccgg
     1801 cgccgaatgg cagatggtct accccgacgc cgcgcagcac gaggtcatct gccacaacga
     1861 cttcgcgccc tacaacgtcg tctatgtggc cgagcagccg cacgcgatca tcgacttcga
     1921 tctgaccggc cccggcccgc gcctctggga tctggcctac gcggcctatc gcttcgtgcc
     1981 gctgtcctat gccgcggata tgcaggagct agggctgtcg gacccgacac agcaaagccg
     2041 ccgcctgcaa ctcttctgca cagcctatgg cctcgccgcc ccgccgggct tgctccacat
     2101 ggtggcgcaa cgcatcgagg ccatgtgcga cctgctcgcc agccgggccg ccgcgggcga
     2161 tcccgccttc cgcaaaatgg tagacgaggg ccatctcgcg tattatcagc gcgaactggc
     2221 cgccttccac gcgtggcagc ccacgctggc gtgcctgctc ggctggtgag gg
//
LOCUS       DASUYC010000574         2214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1780326, whole genome shotgun sequence.
ACCESSION   DASUYC010000574 DASUYC010000000
VERSION     DASUYC010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1780326"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            255..710
                     /locus_tag="VF276_09410"
     CDS             255..710
                     /locus_tag="VF276_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607116.1"
                     /translation="MNDTGWWVTLLGGIGTILGIILKWRTDVRKLTLSGEQRLRDDLF
                     KALDTQKALHAEEIAAVRAEAKREQRALSGEIKTLRSHVLKSQSDITTLNSQLYQMST
                     WASEVHRIALLLYPVEWAQILAQVRVPRPDWQPRPPDLLPPAEEGPADE"
     gene            703..1302
                     /locus_tag="VF276_09415"
     CDS             703..1302
                     /locus_tag="VF276_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607117.1"
                     /translation="MNNLPSTPADFLAALLAGGTLYGLVSTWLIDLLIRAWERRAQGR
                     ISETKRRWVSIGVAIAVPVLAYGGLCALGNAVWSVDGLYAAAMAGVAAVGGSKISYAG
                     RESLSKPGEDAAYRARNDASGDPDAPNGGHVAPQPERLASVQWFPVTPEQQAEYDRQR
                     AALPTAQEVALRAVPAPDHAPDPAAGRLRSGTSWRRIWG"
     gene            1337..1924
                     /locus_tag="VF276_09420"
     CDS             1337..1924
                     /locus_tag="VF276_09420"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX6607118.1"
                     /translation="MGNLTPGPYTIDCLTWDKTATLEIGQGYDLRPPDTVIDALVLHS
                     TNGNGANFNAECAYLRDARDKSTHYMVGEKGQIGRLLDPRYRAWHAGLSLYTEARIWP
                     GLPKPSPYDGRRAWNDFSIGIELVHKVGTEYPLAQLTALRWLVNELRANYRAIQRMGH
                     VAHRWIAVDQYDHYGRKVDPSDMTDLWLRNWIQSL"
BASE COUNT          432 a          720 c          726 g          336 t
ORIGIN      
        1 ctgaggatca tggcccacca ccaacacaac ggggccagca cgagcccggc ggccagcacg
       61 gcaccgactc cagcaagtag cagccgccaa ttgatccgac tcatagcccc agtataccac
      121 aaatggagcg cgcgctaggc atgattgttc tattggcacg ccgcatcccg gcccccgcgc
      181 cgccgggact gggataagga cgggcacatg cgatggacta tcgcgctgct gtactgggcg
      241 caagtgaggc gccgatgaat gataccggct ggtgggtgac gctgctgggc ggcatcggaa
      301 cgattttagg catcatccta aaatggcgca ccgatgtccg caaactgacc ctgagcgggg
      361 aacagcgctt acgggacgac ctgttcaagg cgctggatac ccagaaggcg ctccatgcgg
      421 aggaaatcgc cgccgtgcga gcggaggcca aacgcgaaca gcgcgcgctc agtggggaga
      481 ttaaaacgtt gcggagccat gtgctcaaaa gccagagcga tattaccacc ctgaatagcc
      541 aactctacca gatgagcacg tgggccagcg aagtccaccg gattgccctg ctgttgtatc
      601 cggtggagtg ggcgcaaatc ctggcgcaag tgcgtgtccc ccggcccgac tggcagccgc
      661 gcccgcccga tctgctaccc cccgccgagg aaggacccgc cgatgaataa cctacctagc
      721 acccccgccg acttcctggc cgcgctcctc gcgggcggga cgctgtacgg cctggtgtcc
      781 acctggctca ttgacttgct gatccgcgcc tgggaacgcc gggcgcaggg gcgcatcagt
      841 gagaccaaac gccgttgggt cagtatcggc gtcgctatcg cggtcccggt gctggcctat
      901 ggcggcttgt gtgcgctcgg caatgccgtc tggagtgtag acggcctcta tgccgccgcc
      961 atggccgggg ttgcggcggt cgggggcagc aagattagct acgccgggcg cgagagcctg
     1021 agcaagccgg gcgaggacgc ggcctaccgc gcgcgcaacg atgccagcgg cgaccctgat
     1081 gcgccgaacg gcgggcacgt ggcgccgcag cccgaacgcc tcgcctcggt gcaatggttc
     1141 ccggtcacgc cggagcaaca ggcggagtac gaccgccagc gggccgccct gcccacggcc
     1201 caggaggtag cgttgcgcgc cgttcccgcg ccggatcatg cgcccgatcc ggcagcgggg
     1261 cgccttcgct ccgggacaag ctggcggcgg atatggggct agaggatgag gatacgcggc
     1321 cagaggatac gatctaatgg gcaacctgac accggggccg tacacgattg actgcctgac
     1381 ctgggataaa accgccacgc tggagatcgg gcaggggtat gacttgcgcc cgcccgatac
     1441 cgtgattgac gccctcgtgc tgcacagcac gaacggcaac ggcgccaact tcaacgcgga
     1501 atgcgcgtac ttgcgggacg cccgcgacaa gagcacgcac tacatggtcg gcgaaaaggg
     1561 acaaatcggg cgcctgctgg acccccggta ccgcgcctgg cacgcggggc tgtcgcttta
     1621 taccgaggcg cgcatctggc cgggcctgcc gaagcccagc ccgtatgatg ggcgccgggc
     1681 ctggaatgac ttcagcatcg ggattgaact ggtgcataag gtcggaaccg agtacccgct
     1741 ggcgcaactc acggcgctac ggtggctcgt caatgagttg cgcgctaatt accgggcgat
     1801 ccagcgcatg ggccatgtgg cgcaccgctg gatcgcggta gaccagtacg accattacgg
     1861 gcgcaaggtc gatccgtccg atatgacgga cctatggttg cggaactgga tacagagtct
     1921 ataggcacaa caaagcccgc gtaccgcgta ttaagagcgg cgttgcgggc tttgggtaca
     1981 tgtggcgcgg aatcgaaccg caccggccag gttactctcc gaatcgaacg gagtcacgcc
     2041 gtcgcgtggg ggcagaccag cccctccaca tgccccttca ttatatagga aggcgacccg
     2101 ccatgcaaga gcacatccgc gcccgcgacc tctgtgagca gcacgccctc gccgcgatcc
     2161 atgccctcgc cgccatactg ggcgccacgg aagccccctt gcgcgatgcc cagg
//
LOCUS       DASUYC010000575         2008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_208408,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000575 DASUYC010000000
VERSION     DASUYC010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208408"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="VF276_09425"
     CDS             complement(<1..851)
                     /locus_tag="VF276_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607119.1"
                     /translation="MADILRTTRDEEGRRREDTTAYQMQQQIDDLRRQMRDALARQQW
                     LEDLYKNTEGQVLQLQMLQERHTQDVAQSMQVRQIEDGRLKQQIAELGQKVEEPMKPI
                     RDLRAQIAELIEARRMDRERTVTDARQYEALQGQIRQLVSQIGVIADAQRQLRDLVHE
                     LDAVNNETRQEIQRVAELQRMEEQRLRRQGVELQEMVEGLRQQFTDINSRSQRVEEVR
                     RQLMEHIEGIRAQLSDSGDHNTRFDSLIQRLEKQVHDNHGVVQERSETIRGQLAAELA
                     ELRQVGD"
     gene            complement(864..>2008)
                     /locus_tag="VF276_09430"
     CDS             complement(864..>2008)
                     /locus_tag="VF276_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEX6607120.1"
                     /translation="IGHPADGGRAAGAAGPWSRFDSPRRMAYTPIMAHDASLQTALIY
                     HPGCLAHETGIHVESPERLKAILRAVEQRFGPPALIAPDPAPLEAIRAVHDPEYIETI
                     QGIARAGGGLWDYDTVISAGSYEAALLAAGAACAAVDQVLDPRGPGAASAFAFGRPPG
                     HHACPDLAMGFCLFNNIAVAARHAQRHYAAIRHILIVDWDVHHGNGTQDIFYSDGTVA
                     YFSTHQWPLYPGTGKWTESGAGAGSGWTCNVPLPPGTDDAGYQAAFEEVLIPFARRFQ
                     PDLILVSAGYDAHYADPLAQMAVSTGGFAMLTGMVSELAADLCGGRLAFVLEGGYDVG
                     ALGSGVVATLERLNGAGGPPMPPLPALLRPVRMAIERAVWHHKLLS"
BASE COUNT          285 a          680 c          678 g          365 t
ORIGIN      
        1 tggtcgccca cctggcggag ctcggccagt tcggcggcca gttgcccgcg gatcgtctcg
       61 gaccgctcct gcaccacgcc gtggttatcg tggacctgct tttccaggcg ctggatcagc
      121 gagtcgaagc gggtgttgtg atcgccgctg tcgctgagtt gcgcccggat gccctcgatg
      181 tgctccataa gctggcggcg cacctcctcc acccgctggg agcgcgagtt aatatccgtg
      241 aattgctggc gcaggccctc caccatctcc tgcagttcca ccccttgccg gcgcaggcgc
      301 tgctcttcca tgcgctgtag ctcggccacg cgctggatct cctggcgggt ttcgttgttc
      361 acggcatcca gttcatgcac caggtcgcgc aactggcgct gcgcgtcggc gatcacgccg
      421 atctgcgaca caagctggcg gatctggccc tggagcgcct cgtactgccg cgcatcggtc
      481 acggtgcgtt cgcggtccat gcgccgggcc tcgatcaatt cggcgatctg cgcgcgcagg
      541 tcgcggatcg gcttcatcgg ctcctcgacc ttttggccca gttccgcgat ttgctgcttc
      601 aggcggccgt cttcgatctg gcgcacctgc atgctttgcg ccacatcttg cgtgtggcgc
      661 tcttgcagca tttgcaactg gagcacctgg ccttcggtgt ttttgtagag gtcttcgagc
      721 cattgctggc gggccagcgc gtcgcgcatt tgccgccgca ggtcgtcgat ctgctgttgc
      781 atctggtagg ccgtcgtatc ttcccgccgc cggccctctt cgtcgcgggt agttcgcagg
      841 atatcagcca tagaggtgtc cctttatgat aggagtttat gatgccagac ggcgcgctcg
      901 atggccatgc gcacgggccg cagcaacgcc ggcagcggcg gcatgggcgg cccgccggcg
      961 ccgttgaggc gttccagggt cgccacgacg ccggagccta acgcgcccac gtcgtagccg
     1021 ccctccagca cgaaagccag gcgcccgccg cagaggtcgg cggccagttc gctgaccatg
     1081 ccggtcagca tggcgaagcc cccggtgctc accgccattt gcgccagcgg gtcggcgtag
     1141 tgcgcatcgt acccggcgga gaccaggatg aggtcgggct ggaagcggcg cgcaaacggg
     1201 atcagcactt cctcgaaggc cgcctggtag ccggcatcgt cggtgccggg cggcagcggc
     1261 acattgcagg tccatccgct gcccgccccg gcgccgctct cagtccactt gcccgtgccc
     1321 ggatacagcg gccactgatg ggtgctgaaa taggccacgg tgccgtcgct gtagaagatg
     1381 tcctgggtgc cgttgccgtg gtgcacgtcc caatcgacga tcaggatgtg gcggatagcg
     1441 gcgtagtggc gttgcgcgtg gcgggcggcc acggcgatat tgttgaacag gcagaagccc
     1501 atggccaggt ccgggcaggc gtggtggccg ggcggccgcc cgaaggcgaa ggcgctcgcg
     1561 gcccccggcc cccgcgggtc gagcacctgg tccacggcgg cacaggccgc ccccgccgcc
     1621 agcaacgcgg cctcgtaact gcccgcgctg atcaccgtat cataatccca gaggccgccg
     1681 cccgcgcggg cgatgccctg gatcgtctcg atatactcgg ggtcgtgcac agcgcggatg
     1741 gcttccaacg gtgccgggtc gggcgcgatc agggccggtg gcccaaagcg ctgttcgacc
     1801 gcgcgcaaaa tcgccttgag ccgttctggg gactcgacgt ggatgccggt ctcatgcgcc
     1861 aggcagccgg ggtgataaat gagcgcagtt tgcagcgacg cgtcgtgggc catgatcgga
     1921 gtataagcca tgcgccgggg gctgtcaaac ctcgaccagg ggccagccgc gcccgccgcc
     1981 cgacccccat ccgccggatg accgatgg
//
LOCUS       DASUYC010000576         5603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_727015,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000576 DASUYC010000000
VERSION     DASUYC010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5603
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727015"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..2218)
                     /locus_tag="VF276_09435"
     CDS             complement(203..2218)
                     /locus_tag="VF276_09435"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEX6607121.1"
                     /translation="MRKPSLLTLLGLALLGLLILGAAGAAAQSGYGGRPPQLTPYPTP
                     GTGEYATPVPPGAVPPAAPAAFAPVSRFGMNLYLTGLERSNAQASALATLAAQGGVKW
                     SREELSWANIEPNAKGQFNWSIYDSRLGLDAANGINVVGMLLTTPKWASTNPGAGDYF
                     WYEPRNYNDYFDFVRAAVSRWKNQIHVWEIWNEPNHQATWNCLNNCDRAARYAALLAG
                     AYQAVKSVDPTARVLIGGIYIHDVNTEGLPFLNRVVAASGGAINVDALSIHTYMPDRV
                     PESIQPNSVVQNFQYRLNMMNDWINGHGGRPGEIWVTEDGRSTCNPCNPNIGVVWSED
                     AQASMLARMYGIAAATPRLAQFDWFQFEDKFNHPTDLFGGMAIVRDDLSLKPAYAAYQ
                     TAQRMLDGAALLGTGPQMIPGGSPQQPDNSDFAGFDYRFTRAGSTFHMVWRVNDSVTI
                     NYPVETAQVDMIDRDGGTRRVAATNGTIPLTISTRPQYIVNVNCTARFSDVCPDNWAY
                     TYVEFLAAHGIISGYSDGTFRPGTTATRGQLAKMIVVARGWSLANPARPSFTDVPTSY
                     PFYRYIETAKSHGVISGYSDGTFRPGNTVTRGQICKMVVSAFGWAITTAGGPHFSDVP
                     RADPFYSYVETAVNHTVVSGYGDGTFRPGNPVTRAQLSKILYQAMNP"
     gene            2651..3487
                     /locus_tag="VF276_09440"
     CDS             2651..3487
                     /locus_tag="VF276_09440"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEX6607122.1"
                     /translation="MMLVLDGVLVAVLVVFALVGARRGPWPEVVALGGLLLGALLADE
                     WGEAWGNDLGSALGFLGPVGARMIVRSVLFLVPLIAVGYGGALLLPRLGKPSVRARLI
                     AALLALFNGAAILAVLLRNWDYSQGATGSPLRNDAVTRFFLEWAGWWPLLLALGGIVA
                     VAVSVLRRPRRVTPPAPVTLPAPLGASKEGPAPAIPAPAGSTIPATSGAPRPASKEAP
                     APASLPVRPAISSTPPATPAGDGNGVPRREPAASDERRCRTCGKVLAPGAAFCPNCGT
                     PV"
     gene            3541..4824
                     /locus_tag="VF276_09445"
     CDS             3541..4824
                     /locus_tag="VF276_09445"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX6607123.1"
                     /translation="MNNNRWLQALIILLVIIAATWIMGQVWMLLIQFASIILLFFLAW
                     LLAFALSPLARRLQAWGVRQLLAITVVYLAMLLVLTVIGFLVFPALADQIQRLIDQTP
                     AYTAELEDLGNESLKQMQNWGVRVEDIHLDPLYGAVGEQVKNVGGSVLNFVTGLAAFL
                     FNAIITLLLSFYFMKDGDRLFNNLVVQLPPSLAGEVQLLGTSIARAFGGFLRGQMVFA
                     LLYAVLNAGIMGAFNLDYVLVASIVAGLAMVVPLVGGLLAIVPPVLVALVTPRATDKW
                     WILLIILVVVQAVMIQVVSPRIMSQAIGMHPLFTVAALLIGLQVAGPWGALFGIPVAG
                     VIHQVSGPYFARLRTFFNVPTPDEPALALHGTVPVTTVSPPLPPPGPAPPAPDPVFVV
                     PVPPSATPNAQAINLIVLARGLSRRMLAGRRRARP"
     gene            4821..>5603
                     /locus_tag="VF276_09450"
     CDS             4821..>5603
                     /locus_tag="VF276_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607124.1"
                     /translation="MRRHLGVLAAYAALTVLFTWPLAAQFSRAVPGGGDAWQHIWNLW
                     WMRTALVDLHTDPFYTTYLYYPQGVSLLFHTLVPLESALTVPFQLLGVDLVPLYNGVL
                     ALSFVLAGYGTWLLVRDLTGHDGAAFVAGFAFAFCPYHLGHLLGHMNLASLQWIPLYL
                     WALLRAVGPPGAPFDPHVRRAGGRLDWRPFGWAALAGLFLAANAFTEWTYVAFLGIFT
                     AGYLLWRLLVDGRRAGWRAWVAGVVPLLALALVFGVLAGPLLI"
BASE COUNT          724 a         1839 c         2030 g         1010 t
ORIGIN      
        1 ccggggcgcg tgagccttat ggctcacgcg cccccttctt tccctagccg ggccggtttg
       61 aaaccggccc ctacaacgcg accgcgggcg ggccggtttg aaacgcgcct ctacaacgcg
      121 accgaatgcg ggccggtttg aaaccggccc ctacagcgcg cccgaaggcg gaccggtttc
      181 aaattggcct cgggatcggc ggctatgggt tcatggcctg gtagaggatc ttgctcaact
      241 gggcgcgcgt gaccgggttg ccggggcgga aagtgccgtc gccgtagccc gagaccacgg
      301 tgtggttcac cgccgtctcg acgtagctgt agaacgggtc ggcgcgcggc acgtcgctga
      361 agtgcggccc gcccgccgtg gtgatggccc agccgaacgc gctgacgacc atcttgcaga
      421 tctggccgcg ggtcacggtg ttgccggggc ggaaggtgcc gtcgctgtag ccgctaatca
      481 cgccgtggct cttggccgtc tcgatgtagc ggtagaacgg gtagctggtg ggcacgtcgg
      541 tgaacgaggg ccgggccggg ttggcgagcg accagccgcg ggctacgacg atcatcttgg
      601 cgagttgccc gcgcgtggcc gtggtgccgg ggcgaaaggt gccgtcgctg tagccggaga
      661 tgatgccgtg cgccgccagg aactcgacgt aggtgtaggc ccagttatcg gggcagacat
      721 cgctgaagcg ggccgtacag ttcacgttca cgatgtactg cgggcgggtg ctgatggtca
      781 gcgggatcgt gccgttcgtg gcggccacgc gccgggtgcc gccgtcgcgg tcgatcatgt
      841 ccacctgggc cgtctccacc gggtagttga tcgtcacgct gtcgttcacg cgccagacca
      901 tgtggaaggt ggaaccggcc cgtgtgaagc ggtagtcaaa gccggcgaag tcggagttgt
      961 cgggctgttg cgggctgccg ccggggatca tctgcggccc ggtgccgagc agcgccgcgc
     1021 cgtcgagcat ccgctgggcc gtctggtagg cggcgtaggc cggcttgagg ctgaggtcgt
     1081 cgcggacaat cgccatgccg ccgaagagat cggtggggtg gttgaacttg tcctcgaact
     1141 ggaaccagtc gaactgagcc agccggggcg tggccgccgc gatgccgtac atgcgcgcca
     1201 gcatcgacgc ctgggcgtct tcggaccaga ccacgccgat gttgggattg cacggattgc
     1261 aggtgctgcg cccgtcttcc gtgacccaga tctcgccggg ccgcccgccg tggccgttga
     1321 tccagtcgtt catcatgttg aggcggtact ggaagttctg caccacactg ttgggctgga
     1381 tcgactcggg cacgcggtcc ggcatgtagg tgtggatgga gagggcgtcg acgttgatcg
     1441 ccccgcccga cgcggccacc acccggttga ggaaaggcag gccctcggtg ttgacatcat
     1501 ggatgtagat gccgccgatg agcacgcggg cggtgggatc caccgacttg acggcctgat
     1561 aggcgccggc cagcagcgcg gcatagcggg cggcgcggtc gcagttgttc aggcagttcc
     1621 aggtggcctg gtggttcggc tcgttccaga tctcccagac gtggatctgg ttcttccagc
     1681 ggctgacggc agcgcgcacg aagtcgaagt agtcgttata gttgcgcggc tcataccaga
     1741 agtaatcgcc ggcgccgggg ttggtcgagg cccatttcgg cgtggtcagc agcatgccga
     1801 cgacgttgat gccgttggcc gcgtcaagcc ccaggcggct gtcgtagatg ctccagttga
     1861 actggccctt ggcgttcggc tcgatattgg cccaggacag ctcttcccgc gaccacttga
     1921 cgccgccctg ggcggccagg gtcgccaggg cgctcgcctg ggcattgctg cgctccaggc
     1981 cggtcaggta gaggttcatg ccgaaccgcg acaccggggc gaaggccgcc ggggcggcgg
     2041 gcgggacggc gccgggcggc accggcgtgg cgtactcgcc ggtgccgggc gtggggtagg
     2101 gcgtgagttg cggcgggcgc ccgccatagc cgctttgcgc ggcggccccc gcggcaccca
     2161 ggatcaggag gcccagcagc gccaggccca ggagtgtcag caatgatggt ttgcgcatgt
     2221 ttacccttcc taactcttgc gcccggccag gccggggcag cagtcgcgta gcggtacagc
     2281 gttgtgagta taccgaattg ccgaaagccg ccgcaaatca ggcgggttgc ttggcccggt
     2341 tgcccggccc atgaatgggc gggctaccct tacgaagccc gcctgcgcgg gctgggcatc
     2401 ccggcgggcg gggcaacctc tcccccagcc cctccccgcc gcggggaggg gagttacttt
     2461 tcctcgctgc ggagattccc cccttcccgc ggcgggaagg ggggctgggg ggttaggttg
     2521 ctcgcccacc gggatgccgc ccggcgcggg caaaagcgta acgggcaccc gctgcaacag
     2581 aggtgcctgc taatccgtag caaatgtgat acaatatgct tgtttagcaa gaggaggcgg
     2641 cagagaggcg atgatgctgg tgctcgatgg cgtgctggtg gcggtgctgg tagtgtttgc
     2701 gctggtgggg gcccggcggg gcccgtggcc ggaggtcgtg gcgctgggcg gcttgctgct
     2761 gggcgcgctg ctggccgacg agtggggcga agcctggggc aacgacctgg ggtcggcgct
     2821 gggcttcctg ggccctgtgg gcgcgcggat gatcgtccgc agcgtgctgt tcctggtgcc
     2881 gctgatcgcc gtgggctacg gcggggcgct gctgttgccg cgcctcggca agccgagcgt
     2941 ccgcgcccgc ctgatcgccg ccctgctggc gctgttcaac ggcgcggcca tcctggccgt
     3001 gctgctgcgc aactgggact acagccaggg cgccaccggc agccctctgc gcaacgacgc
     3061 cgtgacgcgc ttcttcctgg agtgggccgg gtggtggccg ctgctgctgg ccctcggcgg
     3121 catcgtcgcc gtggcggtga gcgtgttgcg ccgcccgcgc cgcgtgacgc cgcccgcgcc
     3181 ggtcacactg cctgcccctt taggggcatc gaaagagggc ccggcgccgg cgatcccggc
     3241 gccggccggc agcactatcc ccgccaccag cggggcgccc cgccctgcct ctaaagaggc
     3301 accggcgccc gcgtccctgc cggtgcgccc ggcgatcagc agcaccccgc ccgcgacccc
     3361 cgcgggcgac ggcaacggcg tgccccggcg cgagccggcg gccagtgacg agcgccgctg
     3421 ccgcacctgc ggcaaagtcc tggcgcccgg cgccgccttc tgccccaact gcgggacccc
     3481 tgtctaggtc cgtgcccggt cgggtgcgcg ggccggttct agaaagcaag gcaggtctgg
     3541 atgaataata atcgctggct gcaggcgttg atcatcctgc tggtgattat tgcggcgacc
     3601 tggatcatgg gccaggtctg gatgctgttg atccagttcg ccagtatcat cctgctgttc
     3661 ttcctggcct ggctgctggc ttttgcgctc agtccgctcg cccgccggct gcaagcctgg
     3721 ggcgtgcgcc agctcctcgc catcaccgtc gtctacctgg cgatgctgct cgtgctcacg
     3781 gtgatcggct tcctggtctt ccccgcactc gccgaccaga tccagcgcct gatcgatcaa
     3841 acgcccgcgt acaccgccga actggaggac ctgggcaacg aaagcctgaa gcagatgcaa
     3901 aactggggcg tgcgggtgga agacatccat ctggacccgc tctacggcgc ggtgggcgag
     3961 caggtgaaga acgtcggcgg gagcgtgctc aactttgtca ccgggctggc cgcgttcctg
     4021 ttcaacgcga ttatcaccct gttgctctcg ttctacttta tgaaggatgg cgaccggctg
     4081 ttcaacaacc tggtggtgca actgccgccc tcgctggcgg gcgaggtgca actgctgggc
     4141 acgtccatcg cccgcgcgtt tggcggcttc ctacgcgggc agatggtgtt cgcgctgctg
     4201 tatgccgtgc tcaacgccgg catcatgggc gcgtttaacc tggactacgt gctggtcgcc
     4261 tccattgtcg ccgggctggc gatggtcgtg ccgctggtgg gcgggctgct ggcgattgtg
     4321 ccgcccgtgc tggtggcgct ggtgacgccg cgggcgacgg acaagtggtg gatcttgctg
     4381 atcatcctgg tggtggtgca ggcggtgatg atccaggtgg tcagcccgcg catcatgagc
     4441 caggccatcg gcatgcaccc gctgttcacc gtggcggcgc tgctgatcgg gctgcaggtg
     4501 gccgggccgt ggggcgcgct gttcggcatc ccggtggccg gggtgatcca ccaggtgtcc
     4561 ggcccctatt tcgcccgctt gcgcaccttc ttcaacgtgc cgacgcccga cgaacccgcg
     4621 ctggccttgc acggcacggt ccccgtcacg acggtgtcgc cgcccctgcc gccgcccggc
     4681 ccggctccgc ccgcgcccga tcccgtcttc gtcgtgccgg tgccgcccag cgcgaccccc
     4741 aatgcccagg ccattaacct gatcgtcctg gcccgcggct taagccggcg gatgctggcc
     4801 ggacgacggc gcgcccgccc ttgaggcgac acctcggcgt cctggcggcc tatgccgcgc
     4861 tgactgtttt gttcacctgg ccgctggcgg cccagtttag ccgcgccgtg cccggcggcg
     4921 gcgacgcctg gcagcacatc tggaacctct ggtggatgcg caccgccctg gtggatctgc
     4981 acaccgaccc gttctacacc acctacctct attacccgca gggcgtgagc ctgctgttcc
     5041 atacgctggt gccgctggag agcgcgctga ccgtgccgtt ccagttgctg ggggtggacc
     5101 tggtgccgct gtacaacggc gtgctggcgc tgtcgttcgt gctggcgggc tacggcacct
     5161 ggctgctggt gcgcgacctc accggccacg acggcgcggc gttcgtggcc gggttcgcct
     5221 tcgccttctg cccctaccac ctgggccacc tgctggggca catgaacctg gcgtcgttgc
     5281 aatggattcc cctctacctg tgggcgctgt tgcgggcggt cgggccgccg ggcgcgccgt
     5341 tcgacccgca cgtccgccgg gccggggggc ggcttgattg gcggcccttc ggctgggcgg
     5401 cgctggccgg gctgttcctg gcggccaacg ccttcaccga atggacctac gtggcgttcc
     5461 tgggcatctt caccgcgggc tacctgctgt ggcggctgct ggtcgatggg cggcgtgcgg
     5521 ggtggcgggc ctgggtagcg ggggtggtgc ccttgctggc gctggccctg gtcttcggcg
     5581 tgctggccgg gccgctgctg atc
//
LOCUS       DASUYC010000577         2027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_969854,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000577 DASUYC010000000
VERSION     DASUYC010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969854"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="VF276_09455"
     CDS             <1..899
                     /locus_tag="VF276_09455"
                     /inference="COORDINATES: protein motif:HMM:NF022022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HEX6607125.1"
                     /translation="GDEQALALGTEEPLDQKVIPFMGDDLVAVLTASEDIYISLPGMC
                     RALGLSTQPQLRRIQRSEVLARGLRRISLDTPGGRQEANCLRVDKLALWLAGVETSRI
                     KEEYRAKVIAYQEELAPVAMRVFLRFAGIPTTQLVPATDPRLAALAEQYDTLMDVASF
                     LREHMEAIRTTGEHIAGISIRLDEAVQLLESLTSRQQALATRQDDTEVRVARIDERTQ
                     GLSPAHQRQIQTAVDRMVRETQHNAVPLTYAIIYGRLKSRFLVGSYKEIADEKFEDVL
                     AYLAGELRRATGGRSPEQGRLF"
     gene            937..1848
                     /locus_tag="VF276_09460"
     CDS             937..1848
                     /locus_tag="VF276_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018332096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6607126.1"
                     /translation="MPAERAHTIEFGWFMLGGAVPDHTSVPLLMAEEPAILPLVAEHF
                     DSIWVPDHFYAFDDPAHAWIECWTALTWLAARFPRLHAGPIVLGVGYRHPALLAKMAA
                     TLQVLSGGRFIMGIGAGWREAEYAAYGYPFPAPAVRIQQLAEAVEIMRRMWTEPAPTF
                     TGRHFQIAQAYCEPRPNPPPPIMIGGGGEKLLLPLAGRVADIWDRYHGGSLDAIDRAD
                     YRHKWEIVQRSATEAGRDPAAIRQSYTIETGHLPESAAGSAQWRDALRPLIDLGVRQF
                     ILGFTHVTGTDPLRRLADEVLAPLRAG"
BASE COUNT          317 a          710 c          700 g          300 t
ORIGIN      
        1 caggggacga gcaggccctg gccctgggca cggaagaacc gctggaccag aaggtgatcc
       61 cgttcatggg cgacgacctg gtggcggtgc tgacggcctc ggaggacatt tacatcagcc
      121 tgcccggcat gtgccgcgcc ctggggttga gcacgcagcc ccaactgcgc cgcatccagc
      181 gcagcgaagt gctggcgcgc ggcttgcggc gcatctcgct ggacacgccg ggcgggcgcc
      241 aggaggcgaa ctgcctgcgc gtcgataagc tggccctctg gctggcgggc gtggaaacca
      301 gccgcatcaa agaggagtac cgcgccaagg tgatcgccta ccaggaagaa ctggcccccg
      361 tcgccatgcg cgtcttcttg cgcttcgccg gcatccccac gacccagttg gtgccggcga
      421 ccgacccccg cctggcggcg ctggccgagc agtacgacac gctgatggat gtggcctcgt
      481 tcttgcgaga gcacatggag gcgattcgca ccaccggcga gcacatcgcg ggcatctcga
      541 tccggctcga cgaggcggtg caactgctgg agtcgttgac cagccggcag caggcgctgg
      601 cgacgcggca ggacgatacg gaggtgcgcg tggcgcgcat cgatgaacgc acgcagggct
      661 tgagcccggc ccaccagcgc cagatccaga ccgccgtcga ccgcatggtg cgcgagaccc
      721 agcacaacgc cgtcccgctc acctacgcca tcatctatgg gcggctgaaa tcgcgcttcc
      781 tggtcggttc ctacaaggag atcgcggacg agaagttcga ggacgtgctg gcttacctgg
      841 cgggcgaatt gcgccgggcc accgggggcc gatccccgga acagggccgc ctgttctgag
      901 ccgcggggcg ccgggggcgc cggagaggag aacaccatgc ccgcagagcg cgcacacaca
      961 atcgagtttg gctggttcat gctcggcggc gccgtgcccg atcacaccag cgtgccgctg
     1021 ctgatggccg aggagccggc catcttgccg ctggtggccg agcatttcga ttcgatctgg
     1081 gtgcccgacc atttttatgc ctttgacgat ccggcgcacg cctggatcga gtgctggacg
     1141 gcgctgacct ggctggccgc gcgcttcccg cgcctgcacg ccgggccgat tgtgctgggc
     1201 gtgggctacc gccacccggc gctgctggcg aaaatggccg ccacgttgca ggtgctgagc
     1261 gggggccggt tcatcatggg catcggcgcg gggtggcgcg aggctgaata cgcggcctac
     1321 ggctacccgt tcccggcgcc cgccgtgcgc atccagcaac tggccgaggc ggtggagatc
     1381 atgcgccgga tgtggaccga gcccgcgccg accttcacgg gccgccactt ccagatcgcc
     1441 caggcgtatt gcgagccgcg cccgaacccg cccccgccga tcatgatcgg gggcggcggc
     1501 gaaaagctcc tgctgcccct ggccgggcgc gtggccgaca tctgggaccg ctatcacggc
     1561 ggctcgctgg acgccatcga ccgggccgac taccggcaca agtgggagat cgtgcagcgc
     1621 agcgccaccg aggcgggccg cgacccggcg gcgatccggc aaagctacac catcgagacc
     1681 gggcacctgc ccgaatccgc cgccggctcg gcccaatggc gcgacgccct gcgcccgctg
     1741 atcgatctcg gcgtgcgcca gttcatcctg ggcttcaccc atgtcaccgg caccgacccg
     1801 ctgcggcgcc tggcggacga agtgctggcg cccttgcgcg ccggctgacg ccgagcaccc
     1861 cgtttatcat tgtaggggcc ggtttcaaac cggctcgccg cccatctaac gttgtagggg
     1921 ccggtttcaa accggcccgc cgcccgtcta tcgttatagg ggccggctcg caaccggccc
     1981 gttgcccatc gaacgttgta ggggccggtt gcaaaccggc ccgccgc
//
LOCUS       DASUYC010000578         2219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1188252, whole genome shotgun sequence.
ACCESSION   DASUYC010000578 DASUYC010000000
VERSION     DASUYC010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188252"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1120
                     /locus_tag="VF276_09465"
     CDS             <1..1120
                     /locus_tag="VF276_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX6607127.1"
                     /translation="GVINSHVTQRVAPHGYFYAPDPSSQSVCTIGGNVAENSGGAHCL
                     KYGFTANHVLGLEVVLPDGTMMHLGGKTLDRPGYDLPGIFVGSEGTLGIATKVTLRLV
                     KNPEKVLTLLAAFDTTDEAGEAVSRIIAAGILPAAIEMMDRLSIEAAEAAVHANYPRC
                     GGLLLVELDGPITEVDLLMEQVEAICRACGAWEIRLAHSGTERMLVWKGRKAAFAAMG
                     RISPNYIVQDGVIPRTALPQVLRDIETLSAAAGLRVANVFHAGDGNLHPMVLYDRKIA
                     GQEHAAEELAFSILRRCVEAGGSITGEHGVGQEKKAFMPVMFAEPDLETMQRLRCAFD
                     PRRLANPDKVFPRPRLCGEKTGAYEPHPLETAGLAEFF"
     gene            1214..>2219
                     /locus_tag="VF276_09470"
     CDS             1214..>2219
                     /locus_tag="VF276_09470"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX6607128.1"
                     /translation="MTMQLAALESALGTIVGAEWVRPAGPEDAVAGVRPAWVVEPEGA
                     GEVAAVLRAATDAGAHLAPRGGGTKIGWGLPPRALDAVVSLRRLDAVLEHAWGDLTVT
                     VQAGCPVSVLQQTVAQHGQRVAIDPLWPDRATMGGILAANDTGALRVRFGGLRDLVLG
                     VTIALPDGTLAKSGGKVVKNVAGYDLPKLMTGALGTLGVVVEATFRLYPLPAATRTFT
                     VRAATPAALNTLILRVLDSPLVPTGVQLRVDAGGAAAADVRFEGITGALDIAAAQFRD
                     IAAALGCGDALAEADPAVWQAREALWADSDGALIAKVSTLPAELAACCATGVQAGGNA
                     G"
BASE COUNT          325 a          857 c          751 g          286 t
ORIGIN      
        1 gggcgtcatt aacagccacg taacgcagcg cgtcgcgccg cacggctact tttacgcgcc
       61 cgacccctcg tcgcagtcgg tctgcacgat cggcggcaac gtggccgaga actccggcgg
      121 cgcccactgc ctcaagtacg gcttcaccgc caaccacgtg ctgggcctgg aggtcgtcct
      181 gcccgacggc accatgatgc acctgggcgg caagacgctc gaccgccccg gctacgacct
      241 gcccggcatc ttcgtcggct cggaaggcac gctgggcatc gccaccaagg tcacgctgcg
      301 cctggtcaag aacccggaga aggtgctgac cctgctggcc gccttcgaca ccaccgacga
      361 ggcgggcgaa gccgtcagcc gcatcatcgc cgcgggcatc ctgcccgccg ccatcgagat
      421 gatggaccgc ctgagcatcg aggccgccga ggccgccgtc cacgccaact acccgcgctg
      481 tggcggcctg ctgctggtcg aactcgacgg cccgatcacc gaggtggacc tgctgatgga
      541 gcaggtggaa gcgatctgcc gggcctgcgg cgcctgggag atccgcctgg cgcacagcgg
      601 caccgagcgg atgctggtct ggaagggccg caaagccgcc ttcgccgcca tgggccgcat
      661 ctcgcccaac tacatcgtgc aggacggagt gatcccgcgc accgccctgc cgcaagtctt
      721 gcgcgacatc gagaccctga gcgccgccgc cgggctgcgc gtcgccaacg tcttccacgc
      781 gggcgacggc aacctccacc cgatggtgct ctacgaccgc aagatcgccg ggcaggaaca
      841 cgccgccgag gaactggcct tcagcatcct gcgccgctgt gtcgaggcgg gcggctcgat
      901 caccggcgag cacggcgtgg gccaggagaa gaaagccttc atgccggtca tgttcgccga
      961 acccgacctg gagacgatgc agcgcctgcg ctgcgccttc gacccgcggc ggctcgccaa
     1021 cccggacaaa gtgttccccc gcccgcgcct ctgcggcgaa aagaccggcg cctacgaacc
     1081 ccacccgctc gaaaccgccg gcctcgcgga gttcttttag ccccccggcc acgcccgcag
     1141 actcccctcc ccgccgcggg gaggggccgg gggagaggtt ggtggggccg ggggagaggt
     1201 tggagacagc accatgacca tgcagctagc agccctggag agcgcgctcg gcaccatcgt
     1261 cggggccgag tgggtgcggc ctgccgggcc ggaggacgcc gtggccgggg tgcggcccgc
     1321 ctgggtcgtc gagccggagg gcgcgggcga ggtcgccgcc gtgctgcgcg cagccacgga
     1381 cgcgggcgcg cacctggccc cgcgaggcgg cggcaccaag atcggctggg gcctgccgcc
     1441 ccgtgcgctc gacgccgtcg tgtcgctgcg ccgcctcgac gccgtgctcg aacacgcctg
     1501 gggcgacctc accgtcacgg tccaggcggg ctgtcccgtc agcgtgttgc agcaaacagt
     1561 cgcccaacac ggccagcgcg tcgccatcga tccgctctgg cccgaccggg ccaccatggg
     1621 cggcatcctc gccgcgaacg acaccggcgc cctacgcgtc cgcttcggtg gcctgcgcga
     1681 cctcgtcctg ggcgtcacca tcgccctgcc cgacggcacg ctggcgaaaa gcggcggcaa
     1741 agtcgtcaag aacgtggcgg gctacgacct gcccaagctg atgaccggtg ccctgggcac
     1801 gctgggcgtc gttgtggagg ccaccttccg cctctacccg ctacccgccg ccacgcgcac
     1861 cttcaccgtc cgcgccgcca cgcccgccgc gctcaacacg ctgatcctgc gcgtgctcga
     1921 ctcccccctg gtgccgaccg gcgtccaact gcgcgtggac gccggcggcg ctgcggcagc
     1981 cgacgtgcgc ttcgagggca tcaccggcgc gctggacatc gccgccgccc agttccgcga
     2041 catcgccgcc gcgctcggct gcggcgacgc cctggccgag gccgatcccg ccgtctggca
     2101 ggcccgcgaa gccctttggg cggatagcga cggcgcgctc atcgccaagg tcagcaccct
     2161 gcccgccgaa ctggccgcct gctgcgccac cggcgtccag gctggcggca acgcgggcg
//
LOCUS       DASUYC010000579         3224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_746416,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000579 DASUYC010000000
VERSION     DASUYC010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746416"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1434)
                     /locus_tag="VF276_09475"
     CDS             complement(<1..1434)
                     /locus_tag="VF276_09475"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6607129.1"
                     /translation="MVGDESFKDWLKRQRKALDLTQAELAHKAGCSIYTIQKIEEGGA
                     RPSRQLAELLVAALDAPAEERAALIQRARTPGPRSIAATPAARGPAVSTNGNGAAPPA
                     PEGANPYKGLRAFQEADAPDFFGREALTERLRERLGEETALARFLAVVGPSGAGKSSV
                     VRAGLVPALRQHGLPNGSRPVVVELSPGTHPLEELEAALLRVAANPPPSLMEQLRADE
                     RGLARAVKRVLPGDEQSELLLLIDQFEELFTMVPDERVRANFIDSLFSAVADERSRLR
                     VVITLRADFYDRPLLYLPSSELFGWRTEVVGPLAADELYRAITEPAERAGVALEKGLA
                     AAIMGDVGEQPGMLPLMEYALTELYERRASGLMTLAAYKASGGVFGSLARRAEGVYEQ
                     LSGAEQAEARDLLLRLVTPGEGGEDTRRRALLAEVMSAARDQAALQRVLDLYGRYRML
                     TFDRDPLGGGATVEVAHEAPLRSWRRLS"
     gene            1648..2283
                     /locus_tag="VF276_09480"
     CDS             1648..2283
                     /locus_tag="VF276_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607130.1"
                     /translation="MTRKPRTLLLIAFLSGITLLAPRAAFADPPGLPPLTPPPPAFET
                     CRATGAGAICQGRRQFTEDGGPVGIFCGTSANPIELIATPDIATQRATRYYDRDGNLT
                     RRVLHEDFQGTIINPVTGLSVRTNQLDTLTDDPAVPGDLSAFTETSTGTLRFYLPGGG
                     VLVHDAGLSIIGPDGTIFREEGQHDLDAYYGQGDRSVMTALCAALGSPGTP"
     gene            2343..>3224
                     /locus_tag="VF276_09485"
     CDS             2343..>3224
                     /locus_tag="VF276_09485"
                     /inference="COORDINATES: protein motif:HMM:NF025702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HEX6607131.1"
                     /translation="MKRNTLSSLVMLLAVLGLSLAARAAGAGAAPAHDSAAPNDATQA
                     RLRVSQCVYGEPEMDIYLNGRIPVDADIPLAVRQGDVTRYEYLAPGTHSLAVVPSGQD
                     MSKALIGPLDVTLAAGHRYTVVALGQKDEPRHQALVIDETAAAAAIGAGPLDFVHFMV
                     NNIRGLAGLDFYQGGIVRDANVPYGGFKAARWPVGWWKGLKVTVAGAPDQPLDPGFDG
                     DGYNPPALSTLDCFGGTYPGTIGQDFDTHSSANTSPLNAVDLLQAFNAEAGKSGKGSP
                     SNNTFLAAVQAAGLTDLL"
BASE COUNT          508 a         1174 c         1055 g          487 t
ORIGIN      
        1 gctcagccgg cgccacgagc gcagcggcgc ctcgtgcgcc acctccaccg tggccccccc
       61 gcccagcggg tcgcggtcga aggtcagcat gcggtagcgc ccgtagagat cgagcacccg
      121 ctggagtgca gcctgatcgc gcgccgccga catcacctcg gccagcaggg cgcgccgccg
      181 ggtgtcctcc ccgccttccc ccggcgtcac caggcgcaga agcaggtctc gcgcctcggc
      241 ctgctccgcc ccgctcaact gctcgtacac accctcggcg cgccgcgcca gcgaaccgaa
      301 cacgccgccg ctggccttat aggccgccag ggtcatcagc ccgctcgcgc gccgctcgta
      361 cagttcggtc agcgcgtact ccatcagggg cagcatgccg ggctgctcgc ccacgtcgcc
      421 catgatcgcc gccgccagcc ccttctccag ggcgactccc gcccgctcgg ccggttcggt
      481 gatcgcccgg tagagctcgt ctgccgcgag cgggccaacc acttccgtgc gccagccgaa
      541 caactcgctg gacggcaggt agagcagcgg ccggtcgtag aagtcggcgc gcagcgtgat
      601 caccacccgc aggcgactcc gctcgtcggc caccgcgctg aagaggctgt cgatgaagtt
      661 cgcgcgcacc cgctcgtcgg gcaccatcgt aaacagctct tcgaactggt cgatcagcag
      721 cagcaattcg ctctgctcat cacccggcag cacccgtttg acggcgcggg ccagcccgcg
      781 ctcgtcggcc cgcaactgct ccatcaggct cggcggcgga ttggccgcca cgcgcaacag
      841 cgccgcttcc aactcctcca gcgggtgcgt gccggggcta agttccacga cgacggggcg
      901 gctcccgttg ggcagcccgt gctgccggag cgcgggcacc aggcccgccc gcaccacgct
      961 cgacttcccg gccccggacg gcccgaccac ggccaggaag cgcgccagcg cggtttcctc
     1021 gcccagccgc tcgcgcagcc gctcggtcag cgcctcgcgg ccaaagaagt cgggcgcgtc
     1081 ggcctcttgg aaggcgcgca agcctttgta cggattcgcc ccctccggcg cgggcggggc
     1141 ggcgccgttg ccgttggtcg agacggccgg gccgcgcgcc gctggcgtgg cggcgatgga
     1201 tcgggggccg ggcgtccgcg cgcgctggat cagggccgcg cgttcctcgg ccggggcgtc
     1261 cagggcagcc accagcagtt cggcaagctg gcgcgaaggg cgggcgcccc cttcctcgat
     1321 cttctggatg gtgtagatgg agcagccggc cttgtgggcc agctcggcct gggtgaggtc
     1381 cagcgctttg cgctgccgct tcaaccagtc cttgaaggac tcgtcgccca ccatagaccc
     1441 cgctcccttc tgtaggtgtt gctcgtggag cagtgctgaa agggcgcgcc ggccgcgtag
     1501 gtcgcccggt tccgggggtt ttacgagttt ttctacgact tattttacga ccgcaagact
     1561 atcttggcaa ggccttctct gcgacaataa ctgcacggca ccgggagccg cgacgatgaa
     1621 cagttcacat ctgaaaggag caactccatg actcgtaaac cgcgtaccct gctgctaatc
     1681 gcattcctga gcgggatcac gctgctggcg ccccgcgcgg ccttcgccga cccgcccggc
     1741 ctgccgcccc tcaccccacc cccgccggct ttcgagacct gccgggcgac gggcgcaggc
     1801 gcaatctgcc agggacgccg ccagttcacg gaagacggtg ggcctgtggg catattctgc
     1861 ggcacatccg caaatccgat cgagttgatc gcgactcccg acatcgcgac ccagcgagcc
     1921 acgcgctact atgatcggga cggcaacctg acgcgacggg tcctccacga ggacttccag
     1981 ggcacaatca tcaaccccgt gacgggactc tctgtccgca caaaccagct tgatacgctc
     2041 acggatgatc cggctgtgcc gggggacctg agcgctttta ccgagacgag cacgggcacg
     2101 ttgaggttct acctgccggg tgggggcgtc ctggtgcatg acgccgggct ttccatcatc
     2161 gggcccgacg gcaccatctt ccgtgaggag gggcagcacg acctcgacgc ctattatggg
     2221 caaggtgatc gatccgtgat gacagcgctc tgtgccgcgc tgggcagccc cggcacgcca
     2281 tgagagacga tgttcaagtc ggcaccggtt atgacagatt cagcacagaa aggaacaaca
     2341 cgatgaaacg gaatacgttg tcctccctgg tcatgctcct cgccgtcctg gggctgtcac
     2401 tggcagcccg cgcagctggt gccggggccg cgccggcaca cgactcggcg gcgcccaacg
     2461 acgcgaccca ggcccggctg cgggtgagcc agtgcgtcta cggcgagccc gagatggaca
     2521 tttacctcaa cggccggatc ccggtggacg cggatattcc gctggccgtc cggcagggcg
     2581 atgtcacccg gtatgagtac ctggcgccgg ggacgcacag cctggccgtc gtgcccagtg
     2641 gccaggacat gagcaaggcg ctcattggcc cgctcgacgt gaccctggcc gccggccatc
     2701 gctacacggt ggtggcgctc ggccagaagg acgagccgcg ccaccaggcg ctggtgatcg
     2761 acgagacagc cgcggcggcg gccatcggcg cgggaccgct cgacttcgta cacttcatgg
     2821 tgaacaacat ccggggcctg gccggcctcg atttctacca gggcggcatc gtccgcgacg
     2881 ccaacgtgcc ctacggcggt ttcaaggccg cacgctggcc ggtcggctgg tggaagggcc
     2941 tgaaggtcac ggtggccggc gcgcccgacc agccgctcga ccccggcttc gacggcgacg
     3001 gctataatcc gcccgcgctg tccacgctcg actgcttcgg cggtacctat cccggcacca
     3061 tcggccagga cttcgatacg cacagcagcg ccaacaccag cccgctgaac gccgtcgacc
     3121 tgctgcaggc gttcaacgcc gaggcgggga agagcgggaa gggcagcccg tcgaacaaca
     3181 ccttcctggc cgcggtgcag gcggccggcc tgaccgacct gctg
//
LOCUS       DASUYC010000580         2991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_563755,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000580 DASUYC010000000
VERSION     DASUYC010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563755"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2084)
                     /locus_tag="VF276_09490"
     CDS             complement(<1..2084)
                     /locus_tag="VF276_09490"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX6607132.1"
                     /translation="MALTHPSLHTGEDRGTLWLGSARMALLDVAGGVWTLRRNLTALA
                     GRRLTGAVLQQAGAASGAAFARSLGADAGPEAVRECIAAIQAAGLGEFTVESLDWPRG
                     QAVVRGRSTFESWMFTRHGQTPDGPVCAYSGGVLAGIMNALGGRQDVVCVERACQATG
                     ADRCRFELLPATPDTFDADPLLSNQWGLLEILFDRMPMGIAIFDRDLVLRRCNPTWAD
                     FVDHYTTDRNVTVRPGLRFFDLAPEVEQAVAPLLARVLAGETVRQDALRLQSGDLVSY
                     WDAVFTPLIENGEVVGVIDVTTDATERMRAYELMQQKEAQYRGVFEATGDALIINSLE
                     GIVVEANPAACRMHGYTYEEFIGRDPRTFVHADYLDSFREFVETVRAGGQFETQAVNV
                     RKDGSTLPVEVHGAPFLHKGQPHILAVVRDITDRVQAYQTLEQRVDERTRELTTLLEV
                     SNSMASTLELKPLLNLVFDQLKKLVDYRGAALFALETGNLVVLEIRESVLPTQARPAR
                     FLPEKADVYQEVLQARQPVIIDDIWGDSPMAQRFRRQAGGRVGRIFSNARAWLGVPLL
                     VKDHVVGLLRIDHDQPGYYTPRHAALVMAVASQAAITLENARLYERSQELARKNAALA
                     QIASNVAFAGSLEATLHALARNVVQATSAVACAVVLIDEVGETVRLASCWGLPDGYAA
                     AMEGAWRAGVDAP"
     gene            2380..2751
                     /locus_tag="VF276_09495"
     CDS             2380..2751
                     /locus_tag="VF276_09495"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6607133.1"
                     /translation="MIRILLVDDQPVIRHALRTLLTMEPDMTVVEEAPDGRAAVKLAQ
                     ELQPDVVVMDVNMPRLDGPSAAQEMRRVTPNTAVIMLSLHHDEVTRTRALAAGAAAFI
                     EKQAASMNTLMAAIRQAAQPA"
BASE COUNT          496 a          992 c         1017 g          486 t
ORIGIN      
        1 ggcgcgtcga ctccggcgcg ccaggcgccc tccatcgcgg cggcgtaccc gtccggcagc
       61 ccccagcaac tggccaggcg caccgtttcc cccacctcgt cgatcagcac gaccgcgcag
      121 gcgacggcgc tcgtggcctg caccacgttg cgcgccagag cgtgtagggt ggcctccagc
      181 gacccggcga aggcgacgtt cgaggcgatc tgggccaggg ccgcgttctt gcgggccagc
      241 tcctgggatc gctcatagag ccgcgcattt tccagcgtga tcgccgcctg gctggcgacg
      301 gccatgacca gggcggcatg ccgtggcgtg tagtagccgg gttggtcgtg gtcgatacgc
      361 agcagcccga ccacgtggtc cttgaccagc aacggcacgc ccagccaggc gcgcgcgttg
      421 ctgaagattc gccctacccg gccgccggcc tgccggcgga accgctgcgc catggggctg
      481 tcgccccaga tgtcgtcgat gatgacgggc tgccgggcct gcagcacttc ctggtagaca
      541 tcggcttttt cgggcaggaa gcgcgccggc cgcgcctggg tggggagcac cgattcccgg
      601 atctccagca ccactaagtt gccggtttcg agcgcgaaaa gcgccgcgcc ccgataatcc
      661 accagcttct tgagttgatc gaacaccagg ttgagcaacg gtttgagttc cagcgtcgag
      721 gccatgctgt tggagacttc cagcagggtg gtcagttcgc gggtgcgttc gtcaacccgc
      781 tgctccagcg tctggtaggc ttgcacgcgg tcggtaatat cgcggaccac ggccaggata
      841 tgcggctgcc ctttgtgcag gaagggcgcg ccgtggacct ccaccggcag ggtgctgccg
      901 tccttgcgca cgttgaccgc ctgggtctcg aactggccgc ccgcgcggac cgtttccacg
      961 aattcgcgga agctgtcgag gtaatccgcg tggacgaagg tgcgcggatc gcgaccgata
     1021 aactcctcgt aggtgtagcc atgcatgcgg caggcagccg ggttggcttc gaccacgatg
     1081 ccctccaggc tgttgatgat caaggcgtcg ccggtggcct cgaacacgcc gcgatactgg
     1141 gcctcttttt gttgcatcaa ctcgtaggcc cgcatccgct cagtggcgtc ggtggtcacg
     1201 tcgatcacgc cgacgacctc gccgttctcg atcagcgggg tgaacaccgc gtcccaatac
     1261 gaaacaagat cgccgctctg caagcgcagg gcatcctggc ggacggtctc cccggcgagg
     1321 acgcgggcca gcagcggcgc gaccgcctgc tcgacttcgg gcgccaggtc gaagaagcgc
     1381 aggccaggac gcacggttac gttgcggtct gtcgtgtagt gatcgacgaa gtcggcccag
     1441 gtgggattgc agcgccgcaa caccaggtcc cggtcgaaga tggcgatgcc catcggcatg
     1501 cggtcgaaca agatctccag caagccccac tggttgctga gcaatggatc ggcatcgaag
     1561 gtgtccgggg tcgcgggcag caactcgaac cggcaacggt cggccccggt cgcctggcag
     1621 gcgcgctcca cgcagaccac gtcctggcgc ccgcccaggg cgttcatgat cccggccagc
     1681 acgcccccac tgtacgcgca gacggggccg tcgggggtct gcccatgccg ggtgaacatc
     1741 caggactcga aggtggatcg ccctcgcacc acggcctggc cccgcggcca gtccaggctc
     1801 tcgaccgtga actcgcccaa cccggcagcc tggatggcgg cgatgcactc gcgcacggcc
     1861 tccggcccgg cgtcggcgcc gagggaccgg gcgaaggcgg cgccgctggc ggcgcccgcc
     1921 tgctggagga cggcgccggt cagccggcgc ccggccaggg cggtcaggtt gcggcggagc
     1981 gtccagaccc cgccggcgac atcgagcaac gccatgcggg cgctgccgag ccagagcgtg
     2041 ccgcggtctt cgccggtgtg cagcgagggg tgggtaagag ccatagcgtg ttgcgacctc
     2101 agacggggtg gatgggaatg gacggatgga cgctgcgggc cgggggggcg gtaacgggaa
     2161 tgcgatcaca aacggcaacg acaccggcga agtctctccg tctttatact gatttttgac
     2221 ccggccaatg ccccttatta tatccgtcct gtgccgcacg aaccgggcct ccctggccgg
     2281 tataattcag tgagacaact agaacaaatc tccgtgatta tagcacaatg ggtggctacc
     2341 tggctacgaa ctagccggcc cgcggaaagc gagtgaagta tgatccgaat attgctggtc
     2401 gatgatcaac cggtgatccg ccacgcactg cggacgttgc tgaccatgga accggacatg
     2461 acggtggtcg aggaagcgcc ggacggccgc gcggcggtca aactggcgca ggagttgcag
     2521 ccggacgtgg tggtgatgga cgtgaatatg ccgcggctgg acggcccgag cgccgcccag
     2581 gaaatgcgcc gggtcacgcc caacacggcg gtcattatgc tgagcctcca ccatgacgag
     2641 gtgacgcgca cgcgcgcgct ggccgccggg gccgcggcct ttatcgagaa gcaggccgcc
     2701 agcatgaaca cgctcatggc ggcgatccgc caggccgcgc aacccgcctg atcgagagcc
     2761 aggccgccgt cccgtgcttt gcacgggacg gctttttcgc tttcatcccg ccgtctcgcg
     2821 cctgtcgctc ctggttgccg ccaggtattg cccggtggcg gaggccggcg atataatggg
     2881 cgtatgagta ccgccgccgt ctctgaactc ctggatctgc tcgacacggc cttcgagggc
     2941 acggggcatt ccctgctgga caacctccag cccgtcagct ccgacgattg g
//
LOCUS       DASUYC010000581         2383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_939522,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000581 DASUYC010000000
VERSION     DASUYC010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939522"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VF276_09500"
     CDS             complement(<1..627)
                     /locus_tag="VF276_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607134.1"
                     /translation="MTGKARAAGAGDTLPDVALVPANMQAPAEETRTSLYQALLQLKL
                     PQLRAMAAARHVTGRGSQREPLAEALVENMMDAEAQAALIRNLDQPARAILAVASVLQ
                     LNQGNLAAPPLGRVLRGRHASSGEIEAALAHLGEVGLLLPVFDGASRHQAYYGFPAAT
                     DAGLIPLLPEIRAYSGDLPAPHGDPDAASVTAKFDALLDALTRHSYRLA"
     gene            905..2377
                     /locus_tag="VF276_09505"
     CDS             905..2377
                     /locus_tag="VF276_09505"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX6607135.1"
                     /translation="MLIDPASLQLRPRAATPAADEPARVAFVTLGCKVNQSDTQQAIG
                     AFRGAGFQLVPPGAPADVYVVNTCSVTHVADRKSRQWLRRMARGNPDALVVATGCYAS
                     SGGAVLAEMPEVGLVVTPRQGEDLLRLVLEQLPAEVRARAGRPAPLSAPGAERLTPLP
                     DWATTHGDAEADEADEQALWAADAAAGMRTRAMVKVEDGCNDFCTFCIIPFTRGMPRS
                     TPTARVLERVRARLAGGAQEIVLTGVHMGKYGTDHAQRKRAAADPADAPDLPALVARV
                     LAETDVPRLRLTSLEPTDAQAMLPLFADPVAAGRLARHLHLPLQSGCDATLARMRRDY
                     DTAEYARIIAAVRAAVPEMGITTDVIVGFPGETEDEFAATCAFVERMEFMGVHVFPYS
                     GRPGTPALDMPGQVDPQTRQARVHHLTELGRRAHDAFAAQMLGQVFPVLWEEAGPDGG
                     SGLTDNYLRVRTRHPVRYNTTTPARLTGFDATALWAEPLP"
BASE COUNT          330 a          904 c          805 g          344 t
ORIGIN      
        1 ggccaggcga tagctgtgcc gggtcagagc gtccagcagc gcgtcgaact tcgccgtcac
       61 cgatgcggca tcggggtcgc cgtgcggggc gggcaggtcg ccgctatagg cgcggatttc
      121 gggcaacagc gggatcagcc cggcatccgt ggcggcgggg aagccataat acgcctggtg
      181 ccgggacgcg ccgtcaaaga ccggcagcag cagccccacc tcgcccaggt gggccagggc
      241 cgcctcgatc tcgccggaac tggcgtggcg cccgcgcagc acccggccca gcgggggcgc
      301 ggcgaggttg ccctggttta gctgaagcac cgaggccacg gccaggatcg cccgcgccgg
      361 ctggtccagg ttgcggatga gcgccgcctg cgcctctgca tccatcatgt tctcgaccag
      421 cgcctcggcg agcggttcgc gctggctgcc ccgcccggtg acatgccgcg ccgcggccat
      481 ggcccgcaac tggggcagtt taagttgcag caacgcctgg tacaggctgg tgcgcgtttc
      541 ctccgccggc gcctgcatgt tcgccggcac caatgccaca tcgggcaggg tgtctccggc
      601 ccccgccgcc cgtgctttcc ctgtcatgct ctataatcct tcaaggtgta cgtgtcacgc
      661 tgcatcccga tgcgcgatca acctctcccc cgcccctccc cgccgcgggg aggggagtcg
      721 ggtttccccc ttcccgctcc gggaaggggg ccagggggtt aggcccgtcc cctaccgccg
      781 ggcggcgctt gacgcctgtg ccgccccatc ggtataatca ggagaacacc gtatatgttt
      841 gtagcgatag tatcgtcgtt tcgttactac ggtgcaagcg cgctagaatc gagtaaatgc
      901 ggccatgttg atcgatccgg ccagcctgca actccggccc cgcgccgcca cgcccgcggc
      961 ggatgaaccg gcccgcgtcg cttttgtcac gctgggctgc aaagtcaacc agagcgacac
     1021 ccagcaggcc atcggcgcct tccgcggcgc gggcttccaa ctggtgcccc ccggcgcgcc
     1081 cgccgacgtg tatgtggtca acacctgtag cgtcacccac gtcgccgacc gcaagtcgcg
     1141 ccagtggctg cggcgcatgg cccgcggcaa ccccgacgcg ctggtcgtcg ccaccggctg
     1201 ctacgcctcg tccggcggcg cggtgctggc cgagatgccc gaggtgggcc tggtggtcac
     1261 gccgcgccag ggcgaggacc tgctgcgcct ggtgctggaa caactgcccg ccgaggtccg
     1321 cgcgcgcgcg ggccggcccg cgccgctgag cgcgcccggc gccgagcgcc tgacccccct
     1381 gcccgattgg gccaccaccc acggcgacgc cgaggccgac gaggccgacg agcaggcgct
     1441 gtgggccgcc gatgccgccg ccggcatgcg cactcgcgcc atggtcaagg tagaggacgg
     1501 ctgcaacgac ttctgcacct tctgcatcat cccgttcacc cgcggcatgc cgcgcagcac
     1561 gccgacagcg cgcgtactgg agcgcgtgcg cgcccggctg gcgggcggcg cccaggagat
     1621 cgtgctcacc ggcgtgcata tgggcaagta cggcaccgac catgcccagc gcaagcgcgc
     1681 cgccgccgac cccgccgacg cacccgacct gcccgcgctg gtcgcccgcg tgctggccga
     1741 gaccgatgtg ccccgcctgc gcctcacgtc gctggagccg accgacgccc aggccatgct
     1801 gccgctgttc gccgacccgg tcgccgcggg gcggctggcc cgccacctgc atctgccctt
     1861 gcagagcggc tgcgacgcca cgctggcccg catgcgccgc gactacgata cagcggaata
     1921 tgcgcgcatc atcgccgccg tgcgcgccgc cgtgcccgag atgggcatca ccaccgacgt
     1981 gatcgtcggc ttccccggcg aaaccgaaga cgagttcgcc gccacctgcg cgtttgtcga
     2041 gcgcatggag ttcatgggcg tgcacgtctt cccgtattcg gggcggcccg gcactccggc
     2101 gctggatatg cccggccagg tcgacccgca gacgcgccag gcccgtgtcc atcacctgac
     2161 cgagctgggc cgccgggcgc acgacgcctt cgccgcgcag atgctgggcc aggtgttccc
     2221 ggtgctctgg gaagaagccg ggcccgacgg cggcagcggc ctgaccgata actatctgcg
     2281 cgtgcgcacc cgccatccgg tgcgctacaa cacgaccacg ccggcccgcc tgaccgggtt
     2341 cgacgctacg gccctctggg cggagccgct gccctgatgc gcc
//
LOCUS       DASUYC010000582         2993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1188876, whole genome shotgun sequence.
ACCESSION   DASUYC010000582 DASUYC010000000
VERSION     DASUYC010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188876"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1666
                     /locus_tag="VF276_09510"
     CDS             <1..1666
                     /locus_tag="VF276_09510"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HEX6607136.1"
                     /translation="HLIVDFGREIIGYVELEIDAPAGALIDANFFEGIDDTGIFWTWN
                     LRNSFRYTCREGWQRFTSHQRRGFRYGAFTFRNLQRPLKIRYIGTHLATYPVAARGHF
                     AASDETLTKIWEVAAYTVQLCMLDTYVDCPAYEQVYWVGDARNSALVNAVAFGAFDLT
                     DRCVRLTGQSLSDELRAVKPPHLQAMRTHITTSHVVSSWFDEIPMWTFLWVWMAWENY
                     QHTGDRAALAAYYADVRECLARCAGFLTERDLLDIPDVWNLVDWAAQDLERDGEVISN
                     TVLMAQALDYAARMAEVLDSPDAARAHEQLAAHLRTAVNTYGWSEQHQGYVDTVRDQP
                     AYERYLQRCRERGIEPETFEDFQRKQRVSEPTNTLVLQCDTVPAERREAVLRFVLAAK
                     QGQFIGSSPWRARFGNPDEVVPVGSPWFLFFTLETLFKEGYADDALNILREQWNRMLE
                     KGATTFWETFPGFIGTGHWSRSLCHGWSAAPAYFLSTQVLGVTPAAPGYARIRIAPQP
                     FHLRWARGTVPTPRGPVFVSWRLDDAGQMAIEYHAPEGCEVELIHP"
     gene            complement(1704..2279)
                     /locus_tag="VF276_09515"
     CDS             complement(1704..2279)
                     /locus_tag="VF276_09515"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX6607137.1"
                     /translation="MDSQPTAAHPRIVTCDPVPGYSPQIGRYVAQMTEVRADLLHELE
                     GLTLAQLDWHPDEQTESIGTLLLHLDGVEWSWIHEDIFGAASDAYPGNWAEAMPIRVG
                     VPQVQGRPLAAYLDQLAATRARTLAVLRDFTDADLARLVGESEPPPGQERRSTLYTID
                     WIIWHIIEHEATHVGQVELLRRLAPSPAAQQ"
     gene            2521..>2993
                     /locus_tag="VF276_09520"
     CDS             2521..>2993
                     /locus_tag="VF276_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607138.1"
                     /translation="MSQTEPTPAAERHFDTSSSNALADEYGIGAGGTRDLTDLPIVAA
                     PTMSDENFAAELQRRHSPAVPDAGPLYTTCRDFGVNPAVALGFFAKESTFGTDPNGLA
                     KQTLNWGNLATPTDPRRTTGQTVRFHFKVKGVDHFHDFPVYRSWVDGLADFCARLT"
BASE COUNT          481 a         1034 c          994 g          484 t
ORIGIN      
        1 gcacctgatc gtcgatttcg ggcgcgagat catcggctat gtggagctgg agatcgacgc
       61 cccggcgggc gcgctgatcg acgccaactt cttcgagggc atcgacgaca ccggcatttt
      121 ctggacctgg aacctgcgca acagcttccg ctacacctgt cgcgaggggt ggcagcggtt
      181 cacgtcgcac cagcggcgcg gcttccgcta cggcgcgttc acctttcgca acttgcagcg
      241 gcccctgaag atccgctaca tcggcaccca cctggcgacc tacccggtcg ccgcccgcgg
      301 ccacttcgcg gcgtcggatg aaaccctgac caaaatctgg gaggtggcgg cctacacggt
      361 ccagctttgc atgctcgata cctatgtgga ctgtccggcc tacgagcagg tctactgggt
      421 gggcgacgcg cgcaacagcg ccctggtcaa cgcggtggcc ttcggcgcgt tcgacctgac
      481 cgaccgctgc gtgcgcctca ccggccagtc gctgtccgac gagctacgcg ccgtcaagcc
      541 gccgcacttg caggcgatgc gcacgcacat caccacgtcg catgtggtaa gcagttggtt
      601 tgacgagatc ccgatgtgga ccttcctctg ggtctggatg gcctgggaga actaccagca
      661 cacgggcgac cgcgccgccc tggccgccta ttatgcggac gtgcgcgagt gcctggcccg
      721 ctgcgccggc ttcctgaccg agcgcgacct gctcgacatc cccgatgtct ggaacctggt
      781 ggactgggcg gcacaggacc tggagcgcga cggcgaggtg atcagcaaca ccgtgctgat
      841 ggcccaggcg ctcgattacg cggcgcgcat ggccgaagtg ctggacagtc ccgatgcggc
      901 ccgcgcccat gagcaactgg ccgcgcacct gcgcaccgcc gtcaatacct acggctggtc
      961 cgagcagcac cagggctatg tcgataccgt gcgcgaccag cccgcgtacg agcgctacct
     1021 gcagcggtgc cgggagcggg gcattgaacc cgagacgttc gaggacttcc agcgcaagca
     1081 gcgcgtgagc gagccgacca acacgctggt cttgcagtgc gacaccgtgc cggcggaacg
     1141 gcgggaagcg gtgctgcgct tcgtgctcgc ggccaagcaa ggccagttca tcggcagcag
     1201 tccctggcgc gcccgattcg gcaacccgga cgaggtcgtg cccgtgggca gcccctggtt
     1261 cctgttcttc accctggaaa cgctgttcaa ggaaggctac gcggatgacg cgctgaacat
     1321 cctgcgtgag cagtggaacc ggatgctgga gaagggggcg accaccttct gggagacctt
     1381 ccccggcttc atcggcaccg gccactggag ccgcagcctg tgccacggct ggtcggcggc
     1441 cccggcctac ttcctctcca ctcaggtgct cggcgtcacg cccgccgcgc ccggctacgc
     1501 ccgcatccgc atcgccccgc agccgttcca cctgcgctgg gcacgcggca cggtgccgac
     1561 gccgcgcggc ccggtcttcg tctcctggcg cctggacgat gccggccaga tggcaatcga
     1621 atatcacgcc ccggagggat gcgaggttga gctaatccac ccgtaggcgc ggcgaccctc
     1681 gctacaggtt cccggcggcg gggctattgc tgcgccgccg gggagggcgc caaccggcgc
     1741 agcaattcga cctggccgac atgcgtggcc tcatgctcga tgatgtgcca gatgatccag
     1801 tcgatggtat agagcgtgct gcgccgttcc tggcccggtg gcggttcgga ctcgccgacc
     1861 agccgggcca ggtcggcatc ggtgaagtcg cgcaagaccg ccagggtgcg ggcgcgggtc
     1921 gccgccagtt ggtcgaggta agccgccagg ggccgcccct gcacctgggg cacgcccacg
     1981 cggatcggca tcgcctcggc ccagttgccg gggtaggcat cggaggcggc gccgaaaatg
     2041 tcttcatgga tccaggacca ttcgacgcca tccaggtgca gcaaaagcgt gccgatggat
     2101 tcggtctgct cgtccgggtg ccaatcgagt tgcgcgaggg tcagcccctc cagttcatgc
     2161 aataaatccg cgcggacctc ggtcatctgg gccacgtagc gcccgatttg cgggctatag
     2221 ccgggcacgg ggtcgcaggt gacgatgcgc gggtgcgcgg cggtgggttg ggaatccatg
     2281 cgttttcttc tctcctctcc tattccggta gcgtgggcga caacaacccg caaggggtgt
     2341 gccatgcgcc aagcgatgcc gcgtattgta gggtcctgcc cggcaacgtc cgggactttg
     2401 gcgcggtgcg ggccgcctgg gtcgggtgcg cgccgcaagc gggcggggcg ttcaccgggg
     2461 tttcccgtat agttgctgcg gtgtggtcgt ccactgcgcc gcggctgcga aggagcgcac
     2521 atgagccaga cagaacccac ccccgctgcg gagcggcact tcgacacatc cagcagcaac
     2581 gcgctggccg acgagtacgg aatcggcgcc ggcgggacgc gcgacctgac ggacctgccg
     2641 atcgtggccg cgccgacgat gagcgacgag aatttcgccg ccgaactcca gcgccgccac
     2701 agcccggccg tgccggacgc cggcccgctg tacaccacct gccgggactt cggcgtcaac
     2761 cccgccgtcg ccctcggctt cttcgctaaa gaatcgacct tcggcaccga cccgaacggc
     2821 ctcgccaagc aaacgctcaa ctggggcaac ctggcgacgc ccaccgaccc gcggcgcacc
     2881 acaggccaga ccgtgcgctt ccatttcaaa gtcaagggcg tggatcactt ccacgacttc
     2941 ccggtctacc gcagttgggt cgatgggttg gccgatttct gcgcccggct cac
//
LOCUS       DASUYC010000583         2098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_856502,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000583 DASUYC010000000
VERSION     DASUYC010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856502"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..680
                     /locus_tag="VF276_09525"
     CDS             183..680
                     /locus_tag="VF276_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012892983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX6607139.1"
                     /translation="MSTPSGKSDSKRAQLLAAAMAASRESSTTAVFFHTALAEQVGLG
                     ATEAKTLFILGSRGPLTAGEIAKETGLTTASVTSLLDRLESKGCVRRVRDTRDRRRVI
                     VEPNESWLTELAGVFDTLQEAFAPLLEVYTNEELAAIADFLTRAARLSRTAIAEGTLR
                     KPAAQ"
     gene            complement(1226..1987)
                     /locus_tag="VF276_09530"
     CDS             complement(1226..1987)
                     /locus_tag="VF276_09530"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6607140.1"
                     /translation="MDDPTQRFSRRVDNYVKYRPGYPPEVLALVQRACGLTPEAVIAD
                     IGAGTGRLAELFLAQGNRVLGVEPNREMREAGARLLAGYPGYQAVDGRAEATTLPDAS
                     VDFVTAGQAFHWFDPVGARAEFRRILRPGGWVVLVWNVRRTGSTPFLAGFEDLLRTYA
                     PTYPAEQDEGSAEASIRALFGAAPWQTAHFANAQHFDLAGLTGRLRSSSYAPEPGDPT
                     YTPLMDALAALFAAHAVNGQVTLEYDTEVFYGRLG"
BASE COUNT          334 a          749 c          713 g          302 t
ORIGIN      
        1 ttccaggcag cggtgagccg gtcaaacacg gcctggatgg ccgcgatgtc ggcggtggcg
       61 gcgggggtcg ggtgcgcgtg cgtctcggtg gtcatgggtt tgctccttat tactacactg
      121 tatctagtat ctagatttgc ctagatattt ggctaagtgt ataatatccg aggaggcccg
      181 acttgtcaac cccatccggc aaatccgata gcaaacgcgc gcaactgctc gcggcggcca
      241 tggcggccag ccgggaaagc agcaccaccg cggtcttctt ccacacggcc ctggccgagc
      301 aggtggggct gggcgccacc gaggccaaga cgctcttcat cctgggcagc cggggaccgc
      361 tcaccgcggg cgagatcgcc aaggagaccg ggctgaccac ggcctcggtc acgagcctgc
      421 tcgaccggct ggaaagcaag ggctgcgtgc gccgggtgcg cgacacgcgg gatcgccggc
      481 gcgtgatcgt ggagcctaac gagtcgtggc tgaccgagct ggccggggtg ttcgacacgt
      541 tgcaggaggc gttcgcgccg ctgctcgaag tctataccaa cgaggaactg gccgccatcg
      601 ccgacttcct gacccgcgcc gcccgcctct cccgcaccgc catcgccgag ggcacgctcc
      661 gcaaacccgc cgcccagtaa cggcccgccg ggcgcgggca aaaaaatacc cctctatata
      721 atatatccat tttcgaggcc aaaccctgcc acagggcggg cctaacggac gagtgctgag
      781 tgctgagtgc tgagtgctga gtaacgacga gagacggcgc ggggaggggg atgcgcggta
      841 gcaggagcgc ccggcccgtg aacgggcggg ctacgtgaac caaggccgcc tgcgcggcct
      901 cacgtgaagg ggaacgaccc ttgctggggc gctgcgccat gcacgcccac ccgtttcccg
      961 gcccggcacg cccgccccgt cccgatgccg ccccccgccg taggggcgga ccggcgtgtc
     1021 cgccctgtcg ccctaccggc cccgcggcct accgcgcccc agcccgcgca ggcgggcttc
     1081 gtcaacgtag cccgcccctt gaggggtata ccccagccgg gcgctcccgc taccgagtgg
     1141 atagctgctt cataatgcgc ccagcccgcg caggcgggct tcgtcaacgt agcccgcccc
     1201 ttgaggggcc gggcggggcc gcccgctagc ccaaccgccc gtaaaacacc tcggtgtcgt
     1261 actccagcgt cacctggccg ttgacggcgt gggcggcgaa gagggcggcc agggcgtcca
     1321 tcagcggggt gtaggtgggg tcgccgggtt cgggggcgta ggacgacgag cgcaggcggc
     1381 ccgtcagccc ggccaggtcg aagtgctggg cgttggcgaa gtgcgccgtc tgccagggcg
     1441 ccgcgccgaa cagggcgcgg atgctcgcct cggcgctgcc ctcgtcctgt tccgccgggt
     1501 aggtcggggc ataggtccgc agcagatcct cgaagcccgc caggaacggc gtggagccgg
     1561 tgcgccgcac gttccagacc agcaccaccc agccgccggg ccgcaagatg cgccggaact
     1621 cggcccgcgc gcccaccggg tcgaaccagt ggaatgcttg ccccgcggtg acgaagtcca
     1681 cgctcgcatc gggtagggtg gtggcctcgg cacggccgtc caccgcctga tagccgggat
     1741 agccggccag cagccgcgcc ccggcctcgc gcatctcgcg gttcggctcc acgcccagca
     1801 cccggttgcc ctgggccagg aacagctcgg ccagccgccc ggtccccgcc ccgatgtcgg
     1861 cgatcaccgc ctccggcgtc agcccgcacg cacgctgaac cagcgccagc acctcgggcg
     1921 gatagccggg ccggtatttc acatagttgt ccacccgccg cgaaaagcgc tgcgtcggat
     1981 cgtccatgct gcggtctcct ctcatgtgct tgtgcccatc ttacgccgtc cgccccggca
     2041 acacaagccg cacgagatga gcgacgttta ttccaccgcg aaggcgcgaa ggacgcga
//
LOCUS       DASUYC010000584         3978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_46384,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000584 DASUYC010000000
VERSION     DASUYC010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46384"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1135)
                     /locus_tag="VF276_09535"
     CDS             complement(<1..1135)
                     /locus_tag="VF276_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607141.1"
                     /translation="MDVRTLFHAAPPVASSAQGIQARSLRLWWIILTVGAVLPFAVVQ
                     LPPLYDYYHWVFVGHLVPLLLPGARGTAPAVNSLYGLTLLPIPNLGAPVLIALLSLVL
                     PVGVAGRVFLTLCVLAFAYGFAFLVRAIQARPTALELLGFPVAFGYFMYKGYDSYLLS
                     LALAFFAVGTLHRVTCGGTARPARRAIVWLAGLGVGLYLCHLFGWMVFALATGIYTLR
                     LMRQARWRAGLGLGSTVLPALALLGWYTAGSIGGSKLILYPLLSEKILSLVEPLMLFL
                     RTDPFTPPLATFWTNLGGGLLILALLVANLASPLRKQPWLPLLLPAAILGGIALALPI
                     SEFSGDIRPDERFVLPAVMLGLASLRFKPLTVGRGAAMSAVVLL"
     gene            1542..3689
                     /gene="ispG"
                     /locus_tag="VF276_09540"
     CDS             1542..3689
                     /gene="ispG"
                     /locus_tag="VF276_09540"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HEX6607142.1"
                     /translation="MPANGAASAEAAPALPYCADPYMYKRRKTREVMVGNVGVGGNNP
                     IRIQSMTTTLTKDVDATFAQTLRLIEAGCEIVRITTPTTADAKALGEVKRRLRAMGLE
                     TPLVADIHFSPNAAMEAALWADKVRVNPGNYADAKLFKVLEYSDAEYEDELVRIEEKF
                     APLVLRCKERGVAMRIGTNHGSLSDRIMNRYGDTPLGMVESALEFIRICEKYEYYDII
                     VSMKASNPKVMIQAYRLLAARMAELGMEYPFHLGVTEAGNGEDGRIKSAIGIGSLLED
                     GIGDTIRVSLTEDPEFEVPVAYHLAERYTPRGPRPPLHDGKLWPVAHPAPDAALPDLR
                     DAFGYEPRATRAVQIGKSRSGGNLPAQVIVSLAGRLAPMPQALTDVLALARRAGEQGV
                     RADVLSVGLTSQADVDTLGQVTASMRDLRTPFPVVAITGDPALVAPAQAVARGIKLAL
                     IQGHDPAPLLDAYLAVPAAQRKPLWVDLLDTRSERELRSAEEAHDYRIVDELVGIARR
                     LHEAGVEQAMLSMTIDETGYRLRAYRLLDSRLRELGYEYPLHLIATEVDAADEERLLQ
                     TAVSLGALLTDGIGASVQTYGHGDFEKRLVFTYNLLQAAGARLTKTEFVACPSCGRTL
                     FDLQSTTERIKARTGHLVGVKIAVMGCIVNGLGELADADFGYMGGAPGKVNLFVGKEC
                     VEKNVPTEIAVDKLIDLIKAHGRWVEPGVAVED"
BASE COUNT          745 a         1345 c         1306 g          582 t
ORIGIN      
        1 tgagcagaac cacggcactc atcgccgcgc cacgccccac cgtgagtggt ttgaagcgca
       61 ggctcgccaa gcccagcatc accgcaggca gcacaaagcg ctcgtcgggc cggatatcgc
      121 cgctgaattc gctgatcggc agcgccagcg cgatcccgcc gaggatcgcc gccggcagga
      181 gcagcggcag ccagggctgt ttgcgcaggg gagaggccag gttggcgacc agcagcgcca
      241 ggatcagcag cccgccgccc aggttggtcc agaaggtggc gagtggcggc gtgaacgggt
      301 ccgtccgcag gaacagcatg agcggttcga ccagcgacag gattttctcc gacagcagcg
      361 gatagaggat cagcttggaa ccgccgatgc tgcccgccgt ataccagccg agtagcgcga
      421 gagccggaag caccgtgctg cccagcccga gccccgcccg ccaccgggcc tgacgcatca
      481 ggcgcagggt atagatcccg gttgccagcg caaagaccat ccagccgaag agatggcaga
      541 gatacaaccc cacgccgaga ccggccagcc acacgatggc gcgccgggcc gggcgcgccg
      601 tgcccccgca tgtgacccgg tgcagcgtgc ccacggcgaa gaaggccagc gccagcgaga
      661 gcaggtagct gtcgtagccc ttgtacatga agtagccgaa ggccaccggg aagcccagca
      721 attcgagcgc cgtgggccgc gcctggatcg cccgcaccag gaaggcgaac ccgtacgcga
      781 aggccagcac gcagagcgtc aagaagacgc gtccggccac ccccaccggc agcacgaggc
      841 tgagcagggc gatcagcacc ggcgccccaa ggttcgggat cggcagcagg gtcaacccgt
      901 acaggctgtt caccgccggg gcggtgcccc gcgcccccgg caggagcagc ggcaccaggt
      961 gccccacaaa gacccagtga taataatcgt acagcggcgg cagttgcacg accgcgaagg
     1021 gcagcaccgc ccccacggtc aagatgatcc accacaggcg taaactgcgc gcttgaatgc
     1081 cctgagcaga ggacgccacc ggcggcgcgg catgaaacaa cgtgcgaacg tccatacgct
     1141 taacggccct ttcaccgtaa tgctcccgca ggtgcgccag ggcgcacctg ctcacctacc
     1201 tcccacaggc ccatggtaga gaacccaggt tagggcatgg ttagagccgg cggacgaaga
     1261 ttcttccaaa ttgataactt ttgctcgggt atcaggtagg ggagcgccca gggcgtgccc
     1321 ggagggagtg ccgccccgag gcggatttct gcttcgggca actttccggt ataatgaatg
     1381 tggcgccgtg gcgaaatcgc ccggcccgct gcaacgtata tattatgggc ctatttttcg
     1441 tgtgtagatg tagggccggc gtccgagacc cgcgtccggc cagttgggag aggtaagcag
     1501 gtttgagtaa ctcggtgatt ccccagccta tcaagttgcg gatgcccgct aacggtgcgg
     1561 catctgcgga ggcggccccc gcgctgccct attgcgccga cccgtatatg tacaagcgcc
     1621 gcaagacgcg cgaggtgatg gtcggcaacg tgggcgtggg cggcaacaac cccatccgca
     1681 tccagtcgat gaccaccacg ctgaccaagg atgtcgacgc aactttcgcg cagaccctgc
     1741 gcttgatcga ggcgggctgc gagatcgtgc gcatcacgac gccgaccacc gccgacgcca
     1801 aagcgctggg cgaggtgaag cggcgcctgc gcgccatggg actggaaacg ccgctggtgg
     1861 ccgacatcca cttcagcccg aacgccgcca tggaggccgc gctctgggcc gacaaagtgc
     1921 gcgtcaatcc cggcaactac gccgacgcca agctgtttaa ggtgctggaa tactccgatg
     1981 ccgagtacga ggacgagttg gtgcgcatcg aggagaagtt cgcgccgctg gtgctgcgct
     2041 gcaaggagcg cggcgtcgcc atgcgcatcg gcaccaacca cggctcgctc tccgaccgca
     2101 tcatgaatcg ctacggcgac acgccgctgg gcatggtcga gagcgcgctg gagttcatcc
     2161 gcatctgcga gaagtacgag tattacgaca tcatcgtctc gatgaaggcc agcaacccca
     2221 aggtgatgat ccaggcctac cgcctgctgg ccgcgcgcat ggccgaactg ggcatggagt
     2281 atccgttcca cctgggtgtg accgaagccg gcaacggcga agatgggcgc atcaagagcg
     2341 ccatcggcat cggctcgctg ctcgaagacg gcatcgggga caccatccgc gtctcgctga
     2401 ccgaggaccc ggagttcgag gtcccggtag cctatcacct ggccgagcgc tacacgccgc
     2461 gcggcccgcg cccgccgctg cacgacggca agctgtggcc ggtggcccat cccgcgcccg
     2521 acgcggcgct gcccgatctg cgcgatgcct tcggctacga gccgcgcgcg actcgggccg
     2581 tgcagatcgg caagagccgc agcggcggca acctgccggc ccaggtgatc gtttcgctgg
     2641 ccggacggct ggcgcccatg ccgcaggcgc tcaccgacgt gctggccctg gcccgccgcg
     2701 ccggcgaaca gggcgtccgc gccgacgtgc tgagcgtggg cctcaccagc caggccgacg
     2761 tggacacgct cggccaggtg acggcgagca tgcgcgactt gcgcacgccc ttcccggtgg
     2821 tcgccatcac cggcgacccg gcgctcgtcg ccccggccca ggcggtggcc cgcggcatca
     2881 agctggcctt gatccagggc cacgatccgg ccccgctgct cgacgcctac ctggccgtac
     2941 ccgcggcgca gcgcaagccg ctgtgggtcg atctgctcga cacgcgctcc gagcgcgagt
     3001 tgcgcagcgc cgaggaagcc cacgattacc gcatcgtgga tgaactggtg gggatcgccc
     3061 ggcggctgca tgaggccggg gtcgaacagg ccatgctgag catgaccatc gacgagaccg
     3121 gctaccgcct gcgcgcctat cgcctgctcg actcgcggct gcgcgaactt ggctacgagt
     3181 atccgctgca tctcatcgcc accgaggtgg atgccgcgga cgaagagcgc ctgctgcaaa
     3241 cggccgtctc gctcggcgcg ctgctgaccg acggcattgg cgccagcgtg cagacctacg
     3301 gccatggcga cttcgagaag cggctggtct tcacctacaa cctgctgcag gccgcgggcg
     3361 cccgcctcac caagaccgag ttcgtggcct gcccgtcgtg cgggcgcacg ttgttcgatc
     3421 tgcaatcgac caccgagcgg atcaaggcgc gcaccggcca cctggtcggc gtgaagatcg
     3481 ccgtgatggg ctgcatcgtc aacggcctgg gcgaattggc cgatgccgac ttcggctaca
     3541 tgggcggcgc ccccggcaag gtgaacctgt tcgtgggcaa ggagtgtgtc gagaagaacg
     3601 tgccgaccga gatcgccgta gataagctga tcgacctgat caaggcgcac ggccgctggg
     3661 tcgagcccgg cgtcgcagtc gaagactagc ctcgccgccg gcgcgccctt cacgcaaatg
     3721 caaagggcgc gccggatttc tacggccctg cggtgcatag taagcgcccg gccatgaggc
     3781 gggtgtgaag aaccacgcca ctgcgaaccc tgtttccatt cttgtcgcat tgtaatgaat
     3841 taatgacccc gtatcgcgca tccatccctt gacatgccca taaacgatgt gctacgcttg
     3901 actttattag gggatgctcg acggcatccg gggcatattt cgctggcgcg atatgcccac
     3961 cccgtccccc agactcgg
//
LOCUS       DASUYC010000585         3230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_266340,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000585 DASUYC010000000
VERSION     DASUYC010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266340"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1258)
                     /locus_tag="VF276_09545"
     CDS             complement(<1..1258)
                     /locus_tag="VF276_09545"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX6607143.1"
                     /translation="MDGDLDLETITDAIDAGRPLAEILDLVVDRAQWLLDAAETFILL
                     REGDLLVGAAQRGLTGPAHEIRVPVGESIEGQVAARRRPLIMADPLHDPRFRTLPQRS
                     APIAAIIALPLQARGKVLGVLSAATVAPTDFAETLVLLLVLADLASLAIETSRSTARE
                     ARQTRLVDLLRYVSNVDFESDLQAALTPVASRVAEVMGSEKVDIIFLDPERNAFVSLG
                     VSQTPLGHKQRELGLDIVPAPAASLLADVYRIGESVLVPDSLHDARVYRPFIEQLGIR
                     TLMCSPVAVGGERRAILASTSAQPDAFNESDLAVHDLIARRLSLALQHLELTEDLSRL
                     EAERLARLERDNHLDLLAHDVRNPLAAIKGYAQLASRRLHTGDVAYLERALRLINERA
                     DQLDRMMNDMLDVTRMAAGTFSIQRRT"
     gene            1620..2153
                     /locus_tag="VF276_09550"
     CDS             1620..2153
                     /locus_tag="VF276_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008022071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold domain-containing protein"
                     /protein_id="HEX6607144.1"
                     /translation="MTEHTSGQALQDIWPEATCYGCGPANPYGLHIKSYWDSDGETVF
                     CRFQPDLKYNAGFPNVMYGGLVASLIDCHSIWTAIAAAYKAEGRPHGSAPSISYVTGS
                     LQVRYLHPTPLDQEIVLRAHVTEVQGRKTHVACTVRAGDRVTAEGTVVAVRIDDDKAR
                     GVAAARAPQQAGDEPRQ"
     gene            2452..>3230
                     /locus_tag="VF276_09555"
     CDS             2452..>3230
                     /locus_tag="VF276_09555"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX6607145.1"
                     /translation="MADQWIPSQFFFDQNYLSKVGVERPRDILFIVAHHSAGVESSDI
                     PWLTTRGQVSAHKYVSRSGNRYQLVKDENACWACAVDPQYEIRPPRMGDRWARNENWP
                     ALQVEMENSGTDPFTDAQYDAAADWTAAWVQAYGIPADRNNILGHRELTRHPQHQDPN
                     DLWDWTRFMNMVTARLDSAGYVDYYIEGPPTMSRQLFHDTLAANPRSPITDDADRYYD
                     LCMQYRVNPAVALAFFVKESSKGTAGVAVQTQSWGNMRRAR"
BASE COUNT          592 a         1106 c         1046 g          486 t
ORIGIN      
        1 gggtccggcg ctggatgctg aaggtccccg cggccatgcg cgtcacatcg agcatgtcgt
       61 tcatcatgcg atccaactgg tcggcgcgtt cgttgatcag cctcagcgcg cgctccagat
      121 aggcgacatc gccggtgtgc agacgccggc tcgccagttg cgcgtagcct ttgatcgcgg
      181 ccagcgggtt gcggacatca tgggccagca agtccaggtg gttatcgcgc tccagccgcg
      241 ccagcctttc ggcttcgagg cggctcaagt cctcggtcaa ctccaggtgc tgcaaggcca
      301 ggctcaagcg ccgggcgatc aggtcatgca cggccaggtc gctctcgttg aaggcgtcgg
      361 gctgggcact ggtcgaggcc aggatcgccc ggcgctcccc gcccaccgcg accggactac
      421 acatgagcgt gcggatgccg agttgctcga tgaacggccg gtagacgcgc gcatcgtgca
      481 acgagtcggg caccagcacc gattccccga tccggtacac atcggccagc aggctcgccg
      541 ccggggccgg aacaatatcg aggcccagtt cccgttgctt gtgccccagc ggcgtctggc
      601 tgacccccag cgacacgaat gcgttgcgct ccgggtcgag gaagatgata tcgaccttct
      661 cgctgcccat cacctcggcc acgcgcgagg caaccggggt tagcgcggcc tggagatcgg
      721 actcgaaatc gacattcgag acatagcgca gcagatcgac caggcgggtc tggcgggctt
      781 cccgcgccgt gctgcggctg gtttcgatcg ccaggctcgc caggtcggca agcaccagca
      841 acagcaccag ggtctcggcg aaatcggtgg gggccaccgt ggcggcgctc aacacgccca
      901 ggaccttgcc gcgcgcctgc aagggcagcg cgataatcgc ggctatgggg gcggatcgct
      961 ggggcaaggt gcgaaaccgc gggtcatgca gcgggtcggc catgatcaac ggccggcgcc
     1021 gggcggcgac ctggccctcg atgctctcac ccacgggaac gcggatctca tgggccgggc
     1081 cggtcaggcc ccgttgagcg gctcccacca gcagatcgcc ctcgcgcagc aggataaacg
     1141 tttccgccgc gtccagcagc cactgcgccc ggtccaccac caggtcgagg atctccgcca
     1201 gcgggcggcc cgcatcgatg gcatcggtga ttgtttccag atccaggtcg ccatccattg
     1261 tcgagaaggc cgccatggaa ggcaacgggt atgccgatcg ccccgcactg gacgggtgat
     1321 cgctggattc agaaagcgga gacataggag gtccttggct gaaacgcagg gaaagtcgac
     1381 ctgattgcaa tcgacggcag cagatggcgg catgctactc cagaatacca ccatagtata
     1441 aggattggtt acagcggatg tcaagcgcgg gccgcgcacc gggcacagcc cataccggcc
     1501 cgcgccggcc cgcgggggcg cggatcttgc gggcggggaa cggcgccgtc gcgcccccgg
     1561 cacccggccc ggatgatcac ctaccacacc gagctcatga ggacaaggac cccacgcata
     1621 tgaccgagca caccagcggg caagcgttac aggacatttg gccggaggcc acctgctacg
     1681 gctgcggacc cgcgaatccc tatggcctgc acatcaagag ttactgggac agcgacggcg
     1741 aaaccgtctt ctgccgcttc cagcccgatc tcaaatacaa cgccggcttc cccaacgtca
     1801 tgtacggcgg gctggtggcc tcgctgatcg attgccactc gatctggacg gccattgccg
     1861 ccgcgtataa agccgaaggc cgcccgcacg gctccgcgcc cagcatcagc tatgtcaccg
     1921 ggtcgctaca ggtgcgctac ctgcacccca ccccgctgga ccaggagatc gtcctgcggg
     1981 cgcacgtcac cgaggtgcag gggcgcaaga cccacgtcgc ctgcacggtg cgggcgggcg
     2041 accgcgtcac cgccgagggc acggtggtcg ccgtgcgcat cgacgacgac aaagcccgcg
     2101 gcgtggctgc cgcccgcgcc ccgcagcaag ccggcgacga accccgtcag tagccggacg
     2161 cccgccatgt gccgtcgaag aacgcggctg cacaccggcc tgccacgcct tgtaggggcc
     2221 ggttgcaacc ggcccgcgcc ccctcgcgcc ctgtagaggt cgggtgcaaa ccggcccccg
     2281 cgccacgtaa cagccctgcc cgcccaggcg ccaatagtcc ggcagaccct attgtattag
     2341 cctgatttgt cgtatatact taagaacgag gctcatgggc gcggggagag gccgggcgcc
     2401 ggagtagtcc aacggggagc ttcgtagttc cccctcagca ggagggagta cttggctgat
     2461 cagtggatcc cttcgcagtt cttcttcgac cagaactacc tgagcaaagt cggggtcgag
     2521 cgcccgcggg acattctgtt catcgttgcc caccacagtg ccggggtcga gtccagcgat
     2581 atcccctggc ttacgacgcg gggccaggtc tcggcgcaca agtatgtcag ccgcagcggc
     2641 aatcgctacc agttggtcaa ggacgagaac gcctgctggg cctgcgccgt cgatccgcag
     2701 tatgagatcc ggcccccgcg catgggcgat cgctgggcgc gcaacgaaaa ctggcccgcg
     2761 ctgcaagtcg agatggagaa cagcggcacc gatcccttca ccgacgcgca gtatgacgcc
     2821 gccgccgatt ggacggccgc ctgggtccag gcctacggca tccccgccga ccgcaacaac
     2881 atcctgggcc atcgcgaact gacccgccat ccccagcacc aggaccccaa cgacctgtgg
     2941 gactggacgc gcttcatgaa catggtcacg gcccgcctgg acagcgcggg ctacgtggac
     3001 tattacatcg aggggccgcc gacgatgagc cggcagcttt tccacgacac gctggccgcc
     3061 aacccgcgca gccccatcac cgacgatgcg gatcgctact atgatctctg catgcagtac
     3121 cgcgtgaacc cggccgtggc gctggctttc tttgtgaaag agtcgagcaa aggcacggcg
     3181 ggcgtggccg tgcagacgca aagctggggc aacatgcgcc gggcgcgcaa
//
LOCUS       DASUYC010000586         3772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_839800,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000586 DASUYC010000000
VERSION     DASUYC010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839800"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VF276_09560"
     CDS             <1..259
                     /locus_tag="VF276_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEX6607146.1"
                     /translation="VAAMNGVDALVFTAGVGEHSAEVRGAVAGALGWMGVALDEAANA
                     AAHPDTDIATADSRVRLLVIHTREELVVARDTRRVLQSANA"
     gene            344..2047
                     /locus_tag="VF276_09565"
     CDS             344..2047
                     /locus_tag="VF276_09565"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX6607147.1"
                     /translation="MTTTANSGAAEANKRAFYDEVNRFVGWYLAENPVMATEMGLHAH
                     DNRLADTSPEGLARLRLGLEQFLERLPRYRPDGDVDWEIDYRLMAAMAGGRLRALDTR
                     DLAHRSPDFYAAEALFGPYSLLMKEFAPLDERLRNLAGRLAEVPRVLADAERNLDVCP
                     PSWVDGAIESVEGGLALLQHLIPALAQSIRGDDLILAARIEATNTKAVAAVERYRQFL
                     VETLRPRAGGTFAVGEDVWNAMVREEHMLDLDATAIEAIGHELIRETQAALAEEAAAI
                     VAREGGPPRTWQELLNEVRADHPTPDGLVPAYQAAMEASRAFIVEHDLVTLPPDEHLH
                     IAETPPFVRPFYPFAAYMQPGAFEEKQFGIFWVTPVPPGLPAADTESLLRGHPHAKIP
                     IIAVHEGYPGHHVQLSRANGASVPRRLGQSLADLFIEGWAFYCEELMEAEGFLTDPRG
                     RLMRLADQLWRACRIVIDVGLHCRAMSFDAAVQMLVDVARLEPANARAEVQRYTQSPT
                     QPMSYLMGKREILHLAADYRARETGAGRPFALKRFHDTLLACGSLPPRLLRLALFGLA
                     E"
     gene            complement(2139..3086)
                     /locus_tag="VF276_09570"
     CDS             complement(2139..3086)
                     /locus_tag="VF276_09570"
                     /inference="COORDINATES: protein motif:HMM:NF028425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toast rack family protein"
                     /protein_id="HEX6607148.1"
                     /translation="MDTNTRKFSDGTAARVGDAAPNAGPQPAYSPPAYAQPAYAPPAY
                     VPGPPPVPRKRGLSPLVWVLLVLGMLLLLCGGGFVAMAAWITTTAVKVVGDIPTVVVG
                     PLQTETSSIQLDGAHAVDVNLKMGAGNLTLAGGATNLLDGTFTYDVAAWKPEVTYQVN
                     GSTGTLTVRQARENGIVRTLDHPHNEWDLHLQDDVPLTLTASMGAGESKLKLGSLNLT
                     KLDLQTGAGTAMVDLTGNWKQNLTAGIEGGIGDMTVKLPRDAGVRVKVEHSGLGKVQA
                     TDLTVSGDTYTNAAYGHSPVTLDLTVQLGVGNITLEQAQ"
BASE COUNT          624 a         1297 c         1253 g          598 t
ORIGIN      
        1 ggtcgcagcc atgaacgggg tagacgcgct ggtgttcacc gccggggtgg gcgagcactc
       61 ggcggaggtg cgtggggcgg tagccggggc gctcggctgg atgggcgtgg ccctggacga
      121 agcggccaac gcggcggccc accccgacac cgacatcgcc acggccgact cgcgtgtccg
      181 cctgctggtc atccacaccc gcgaggaatt ggtggtcgcc cgcgacacgc gccgggtgtt
      241 gcaatcggca aacgcatgat tatgcggccc ggcgccgcaa ccgcgttgta ggggccggtt
      301 tgaaaccggc ccggcctgcc cggccacgaa ggaaccctga cccatgacca cgacagcgaa
      361 cagcggcgcg gcggaggcga acaaacgcgc cttttacgat gaggtcaacc gctttgtcgg
      421 ctggtatctg gccgaaaacc cggtcatggc gaccgagatg ggcctgcacg cccacgacaa
      481 ccgcctggcc gatacctcgc cggaggggct ggcccgcctg cggctggggc tggagcaatt
      541 cctggagcgc ctgccgcgct accggcccga cggcgatgtg gactgggaga tcgactaccg
      601 gctgatggcg gccatggccg ggggccggct gcgtgccctc gacacgcgcg acctggccca
      661 ccgcagcccc gatttctacg ccgccgaggc gctgttcggc ccctacagcc tgctgatgaa
      721 ggagttcgcg ccgctggacg agcggctgcg caatctggcg gggcgcctgg ccgaggtacc
      781 acgcgtgctg gccgacgccg agcgcaacct cgacgtgtgc ccgccgtcct gggtagacgg
      841 cgcgatcgag tcggtcgagg gcgggctggc cctgctgcaa cacctcatcc cagcgctggc
      901 ccaatcgatc cgcggcgacg acctgatcct ggccgcgcgc atcgaggcga ccaacaccaa
      961 agccgtcgcc gccgtcgagc gctaccggca gttcctggtg gagacgttgc gcccccgcgc
     1021 gggcggtacc ttcgccgtcg gggaagacgt ttggaacgcc atggtgcgcg aggagcatat
     1081 gctcgacctg gacgccacgg cgatcgaagc catcggccac gagttgatcc gcgagacgca
     1141 ggcggcgctg gccgaggaag cggctgccat cgtggcccgc gagggcggcc cgccgcgcac
     1201 ctggcaggaa ctgctgaacg aggtccgcgc cgaccacccc acgcccgacg gcctggtgcc
     1261 cgcctatcag gcggccatgg aagcctcgcg cgccttcatc gtcgagcacg acctggtgac
     1321 gctgccgccc gacgagcacc tgcacatcgc cgagacgccg cctttcgtgc gccccttcta
     1381 ccccttcgcc gcctacatgc agcccggcgc cttcgaggag aagcaattcg gcatcttctg
     1441 ggtaacacct gtaccgcccg gcctgcccgc cgccgatacc gagagcctgc tgcgcggcca
     1501 cccgcacgcc aagatcccga tcatcgcggt ccacgaaggc taccccggcc accacgtgca
     1561 actgagccgc gccaacggcg ccagcgtgcc ccggcgcctg ggccagagcc tggccgacct
     1621 gttcatcgag ggctgggcct tctactgcga ggaactgatg gaggccgagg gcttcctcac
     1681 cgacccgcgt gggcgcctga tgcgcctggc cgaccagctc tggcgcgcct gccgcatcgt
     1741 gatcgacgtg ggcctgcact gccgggccat gtcgtttgac gccgccgtgc agatgctggt
     1801 ggatgtggcg cgcctggaac cggcgaacgc ccgtgccgaa gtgcagcgct acacgcaaag
     1861 ccctacccag ccgatgagct acctgatggg caagcgcgag atcctgcacc tggccgccga
     1921 ctatcgcgcc cgcgagaccg gcgccgggcg gccctttgcg ttgaagcgct tccacgacac
     1981 gctgctggcc tgcggcagcc tgccgccccg cctcttgcgc ctggcgttgt tcggcctggc
     2041 cgaatagtcg cccgcagcct cccggctcag tggaaaaggc caggggcagg agatccgtga
     2101 atctcccacc cctggcgcat gcgctggcta tgctgcgcct attgcgcctg ctccagcgtg
     2161 atgttgccga cccccagctg gaccgtcagg tccaacgtca ccgggctatg cccgtaggcg
     2221 gcgttggtgt aggtgtcgcc ggaaacggtc agatcagtgg cttggacctt gcccaggccc
     2281 gagtgctcca ctttcacccg caccccggca tcgcgcggca gtttcaccgt catgtcgccg
     2341 atcccgcctt cgatcccggc ggtgagattc tgcttccagt tccccgtcaa gtccaccatc
     2401 gcagtgcccg cgccggtctg caggtcgagc ttcgtcaggt tgaggctacc gagcttgagt
     2461 ttgctctccc cggcgcccat gctggcagtc agcgtcagcg gcacgtcgtc ctgcaggtgc
     2521 aggtcccact cattgtgcgg gtgatcgagc gtgcgcacga tgccgttctc acgcgcctgt
     2581 cgcacggtca gcgtgccggt gctaccgttg acctggtagg tcacttcggg cttccaggcg
     2641 gcgacgtcgt aggtgaaggt accgtccagc aggttcgtgg ccccgccggc cagcgtcagg
     2701 ttgcccgcgc ccattttgag gttgacatcc accgcgtgcg cgccgtccag ttgaatcgac
     2761 gaggtctcgg tctgcaacgg ccccacgaca acggtgggga tgtcgcccac gactttaacg
     2821 gcggtcgttg tgatccaggc ggccatggcg acgaacccgc cgccgcacag cagcagcagc
     2881 atccccagca ccagcagcac ccagacgagc ggactcaggc cacgtttccg gggcacaggc
     2941 gggggacccg gcacgtaggc cgggggcgca tacgcgggtt gggcatatgc aggcgggcta
     3001 taggcgggtt gcgggccggc gttgggcgcg gcgtccccga cacgggccgc cgtaccatcc
     3061 gagaatttgc gcgtgttggt atccattaac tttgctccta accggggcgg cctacccgat
     3121 cactccggcg atgccggatc ctccggcgtt ctggttccaa tgatctgttc gaaaagcact
     3181 cgcgcggcgc gaacgtgcgg gatcgcatcc atacgatggg cggccccgcg ggagtgggca
     3241 taagtagatt ataggagagg atgcgcacag gtcatctata caaaaatcga cacaagaatc
     3301 tatccgcagt gaacgaccgg caccctgcaa gatgccgggc tcttcgctgc gctcagcgtg
     3361 acaaggtggg cgggaagccc gacaaaccag tgcggaacct gaatgtggca ggcacgaagc
     3421 aaaaccggcc ataccggcac gaacggcccg ccgatctcgt tactggcgat cgaggtggta
     3481 aatacgctat aaatatggag atctaatccc ggtggtgctg tagcgggacg caagccgcgc
     3541 ctggtcggtc atcacttggc ggggcagtct cacaaagggc cggccatgcc cggcagtatg
     3601 cctagacaag gaactaattg aatgcgtacc aggaaacccg cctggatttc cgttgtactg
     3661 atgctggcgg tcgtggccgt cttattcccg gcgcgagccg gcgccgtggg gagcgccagt
     3721 cccgccgcac agggcagttg ccagaccttc ccggagacgg gcaaaaccgt ca
//
LOCUS       DASUYC010000587         2793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1189663, whole genome shotgun sequence.
ACCESSION   DASUYC010000587 DASUYC010000000
VERSION     DASUYC010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189663"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="VF276_09575"
     CDS             <1..633
                     /locus_tag="VF276_09575"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEX6607149.1"
                     /translation="PYSIAQIVGAFCAAGLLLLNYNPAIAAYEAAHNIVRGAAAGLAN
                     SGTPATATIFATYNNAAAGVQWGNAMLDQIIGTALLMGLILAIIDKRNLPVESNLAPF
                     VIGLLVVVIGMAIGGNAGYAINPARDLGPRLLTSIGGWGLNPFTYTADFFGVALPYFL
                     VPIIGPLIGAAVGAYVYDYGINQYLVARGEPEADVTVRGETVIDKQGATT"
     gene            916..2421
                     /gene="glpK"
                     /locus_tag="VF276_09580"
     CDS             916..2421
                     /gene="glpK"
                     /locus_tag="VF276_09580"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEX6607150.1"
                     /translation="MAETQYILALDQGTTSSRAIIFDHSGHVVAQSNQEFEQIFPRPG
                     WVEHNPEDIWSSQMNVAKKVMQEKNLQASDFAAIGITNQRETAVIWDRATGRPIHNAI
                     VWQDRRTAGFCDDLKRRGWTDKIRDKTGLVIDAYFSGTKVKWLLDNVEGARERAERGE
                     LLFGNIDTFLIWRLTGGEVHVTDYSNASRTMLFNIHDLKWDEDILKEFNIPAAMLPKA
                     TQSSEVYGHSVADLLGGSIPIAGDAGDQQAATFGQACYTPGMAKNTYGTGCFMLMNTG
                     ETAVPSKNNLLTTIGWGLNGKVTYCLEGSVFVTGAAVQYLRDSLKIIGTAAETEALAM
                     SVQDSGGVYVVPAFVGLGAPYWDQYARGAILGLTRGSGRAEITRATLESVAYQSRDLL
                     EAMVADSGDELKELRVDGGMVANNFLMQFQADILGVPVERPQVAETTALGAAYLAGLA
                     VGYWKDQAEIQQNWALDRRFTPQMDEATREKLYKGWKKAVTRARDWEEAEA"
BASE COUNT          491 a          952 c          931 g          419 t
ORIGIN      
        1 ccctattcga tcgcccagat cgtcggcgcg ttctgcgccg cgggcctgct gctgttgaac
       61 tataatcccg ccattgccgc ctacgaagcg gcccacaata tcgtgcgcgg ggccgccgcg
      121 ggtctcgcca attcgggcac ccccgcgacc gccaccatct ttgccaccta taacaacgcg
      181 gcggcgggcg tccagtgggg caacgccatg ctcgaccaga tcatcggcac cgccctgctc
      241 atgggcctga tcctggcgat catcgacaag cgcaacctgc cggtcgaatc caacctggcc
      301 cccttcgtca tcggtctgct cgtcgtggtt atcggcatgg cgattggcgg caacgccggc
      361 tacgccatca acccggcccg tgacctgggc ccgcgcctgc tcacgagcat cggcggctgg
      421 ggcctcaatc ccttcaccta tacggccgat ttcttcggtg tcgccttgcc ctatttcctg
      481 gtgcccatta tcggtccgtt gatcggcgcc gctgttgggg cctatgtcta cgattacggc
      541 atcaaccagt acctggtggc gcgcggcgaa cccgaagccg acgtgaccgt gcgcggcgaa
      601 accgtcatcg acaagcaggg cgccacgact tgacgagtgc tgagtgctga gtacgacgag
      661 tggtcagtgg tcagtggtca gtggtcagtg tggcgggcct attccccccg gccccccttc
      721 ccggcgcggg aaggggggag acgggcgggc ctgttgcccc cggcccctct gcccggcgcg
      781 ggcagggggg agacgggcgg ggcgtggggc ggaccggggt ggccgccggt tgattcgcat
      841 cgggagtgac cgggcgcggc aggggtcgcg cccaccaacc atccgcgctg tgtagcacgc
      901 aaaggagcgc atatcatggc tgaaacccag tatattctgg ccctggacca gggcaccacc
      961 agttcccgcg ccattatctt cgaccacagc ggccatgtgg tggcgcagag caaccaggag
     1021 ttcgagcaga tcttcccccg gcccggctgg gtcgagcata acccggaaga catctggagc
     1081 agccagatga acgtcgccaa gaaggtgatg caggaaaaga acttgcaggc gagcgacttc
     1141 gccgccatcg gcatcaccaa ccagcgcgag accgccgtga tctgggaccg cgcgacgggc
     1201 cggcccatcc ataacgccat cgtctggcag gaccgccgca ccgccggctt ctgcgacgac
     1261 ctcaagcgcc gcggctggac cgacaagatc cgcgacaaga ccggcctggt catcgacgcc
     1321 tatttcagcg gcaccaaggt gaaatggctg ctggataacg tcgagggcgc ccgcgaacgg
     1381 gccgagcgcg gcgagttgct gttcggcaac atcgacacct tcctgatctg gcggctcacc
     1441 ggcggcgagg tccacgtcac cgactactcg aacgccagcc gcaccatgct gttcaacatc
     1501 catgacctga agtgggatga ggacatcctc aaggagttca acatcccggc ggcgatgctg
     1561 cccaaggcca cccagagcag cgaggtctac gggcactcgg tggccgatct gctcggcggc
     1621 tcgatcccga tcgcgggcga tgccggcgac cagcaggcgg ccaccttcgg ccaggcgtgc
     1681 tacacgccgg gcatggccaa gaacacctac ggcaccggct gcttcatgct gatgaacacc
     1741 ggcgagaccg ccgtgcccag caagaacaac ctgctgacca ccatcggctg gggcctgaac
     1801 ggcaaggtca cgtactgcct cgaaggctcg gtgttcgtca ccggcgcggc ggtgcagtac
     1861 ctgcgcgaca gcctcaagat catcggcacc gccgccgaaa ccgaggcgct ggcgatgtcg
     1921 gtgcaggaca gcggcggcgt ctacgtcgtg ccggcgttcg tcggcctggg tgcgccctac
     1981 tgggaccagt acgcccgcgg cgccatcctg ggcctgaccc gcggcagcgg ccgcgccgag
     2041 atcacccgcg ccacgctgga gtcggtggcc taccagagcc gcgacctgct ggaggccatg
     2101 gtcgcggaca gcggggacga gttgaaggag ctgcgcgtgg acggcggcat ggtcgccaac
     2161 aatttcctga tgcagttcca ggccgacatc ctgggcgtgc cggtcgagcg cccgcaggtg
     2221 gccgagacga cggcgctggg cgcggcctac ctggccgggc tggccgtggg ctactggaag
     2281 gaccaggccg agatccagca gaactgggcg ctggaccgcc ggttcacccc gcagatggac
     2341 gaggccacgc gcgagaagct ctacaagggc tggaagaagg ccgtgacccg cgcccgcgac
     2401 tgggaggaag ccgaggccta gcgcaccggg tcgcccgatg gggcgggatc gcccggccat
     2461 gggcgggcgg tagcgggagc acccgccaat gcgcggcgcg cggtagcggc accgcccggc
     2521 ccctaaaggg gcgggctagc acaacgaagc ccgcctacgc gggctgggga tcgtcaatcg
     2581 cagatcaaat gccgggccgg tggcgtaaga tgccgccggc cctgcgccat gtccgccgcc
     2641 atccgtagcg gcgcgattta tcgcgcctgc cgcccctacg cccacctcgt aactcgtcgt
     2701 gacacagcac tcagcactca gcacttccct gaaagtggga aggacggata gccctggggg
     2761 cttgtcattc tgagcgcagc gaagaatctc gtc
//
LOCUS       DASUYC010000588         2498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1189670, whole genome shotgun sequence.
ACCESSION   DASUYC010000588 DASUYC010000000
VERSION     DASUYC010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189670"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1229
                     /locus_tag="VF276_09585"
     CDS             <1..1229
                     /locus_tag="VF276_09585"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX6607151.1"
                     /translation="LADHDTLSPQHMAHLTQLLQQTAFGQRVAAFKVWTKQGRIVYSP
                     EAALVGQQFPVQGGLAGAVAGRVTAEISNLSNAENVLERATQSRLLEMYTPVRRAGAE
                     DVIAVAEVYYSVDDLESEISAAQQRSWLVFGVCTLAMYLLLAGFVRDASRTISRQRTT
                     LSEQVSQLQTLLAQNETLHERVRRAAARTTALNERFLRRVSAELHDGPAQELGLALLR
                     LDHVIAHAAPAHPGPAVSTQPAVAEELEIIQGSLGRAMQEVRAISSGLGLPQMEHWTL
                     AETLSRVVSTHERRTGTKATVHLDCPTVAVPLPVKITLYRLVQEALTNAYRHAGGQGQ
                     QVTVRCAGDILTVEIADAGPGFDGSVDSGPQEHLGLIGMRERVESLGGVFHVESAPGR
                     GTRIMAQFALGAQDER"
     gene            1219..1872
                     /locus_tag="VF276_09590"
     CDS             1219..1872
                     /locus_tag="VF276_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014695521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6607152.1"
                     /translation="MSDKIQIVLADDHPLFRAGVAYTLSAEPDMEVVGQGASAAEAVQ
                     LAQELLPDLILLDLNMPGSGLQAAQAIAATCPVTRIVMLTVSEEEEDLLASLKAGARG
                     YILKGVPARELTRILRAVCAGESYVTPALAASLLREMTGVAPGARPAVDPLNELTDRE
                     RQILEGVANGLSNKEIGQQLHLTEKTVKHYMTNILQKLQVRNRVEAALIARRGAPPR"
     gene            2018..>2498
                     /locus_tag="VF276_09595"
     CDS             2018..>2498
                     /locus_tag="VF276_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607153.1"
                     /translation="MDAFLLSPSAIRNLPILILNLLLAIYLLRGYRRSRATALLALWM
                     CSLAVAFLAILLSRTVYDNPVLSFIANWQVATLAGLAGTVVLLAFAYAFPRNPYPREA
                     RAVLAVAGVVAAGLGVLLLAGIPAGPASYWYEFRRFVWTPHELGLGNVLYPLVLPVGY
                     "
BASE COUNT          402 a          877 c          848 g          371 t
ORIGIN      
        1 aactggccga tcacgacacg ctcagccccc agcatatggc ccacctgact cagctgctcc
       61 agcagacggc gttcgggcag cgggtggccg cattcaaggt ctggacaaaa cagggccgca
      121 ttgtctatag cccggaagcc gcgctggtgg gacagcagtt ccccgtgcaa gggggcctcg
      181 ccggcgccgt cgcaggccgg gtcaccgccg agatcagcaa cctgagcaat gcggagaacg
      241 tgctggagcg ggccacgcag agccggctgc tggagatgta tacccccgtg cgccgggccg
      301 gcgccgagga tgtcatcgcg gtggccgagg tctattactc ggtggacgac ctggagagcg
      361 aaatcagcgc cgcgcagcag cggagctggc tggtgttcgg cgtctgcacc ctggcgatgt
      421 atctgctgct ggccggcttc gtccgcgatg ccagccgcac catcagccgc cagcgcacga
      481 cgctcagtga gcaagtgagc cagttgcaga cgctgctggc ccagaacgag accctgcacg
      541 agcgcgtgcg ccgggcggcg gcccgcacca ccgcgctgaa cgagcgcttc ctgcgccggg
      601 tcagcgccga gttgcacgac ggccccgcgc aagaactggg gctggccctg ctgcggctgg
      661 accacgtgat cgcccacgcc gccccggccc accccggccc ggcggtgagc acgcagccgg
      721 cggtggccga agaactggag atcatccagg gatcgctggg ccgggccatg caagaggtcc
      781 gcgctatctc cagcgggctg ggcctgccgc aaatggagca ctggaccctg gccgagaccc
      841 tgagccgcgt cgtgagcacg cacgaacggc gcacgggcac gaaagccacc gtccacctcg
      901 actgtcccac tgtagccgtc ccgctcccgg tcaagatcac cctgtaccgg ctggtgcagg
      961 aggcgctgac caacgcctat cgccacgcgg gcggccaggg gcagcaggtg acggtgcgct
     1021 gcgccggaga catcctgacg gtggagatcg ccgatgcggg gccgggcttt gacggatcgg
     1081 tggacagcgg cccgcaagaa cacctgggtc tgatcggtat gcgcgagcgg gtcgagagcc
     1141 tcggcggcgt gttccacgtc gagagcgccc ccggtcgcgg cacccgcatc atggcccaat
     1201 tcgccctggg agcgcaagat gagcgataaa atccagatcg tcctggccga tgaccatccg
     1261 ttgttccggg ccggggtagc ctataccctg agcgccgagc cggatatgga agtggtgggc
     1321 cagggcgcgt ccgccgcgga agcagtgcaa ctcgcacagg aactgttgcc cgacctgatc
     1381 ctgctcgatc tgaatatgcc gggcagcggc ctccaggcgg cgcaggccat cgccgctacc
     1441 tgcccggtga cgcgcatcgt gatgctgacg gtgtcggagg aagaggaaga cctgctggcg
     1501 agcctgaagg ccggggcgcg cggctatatc ctcaagggcg tgcccgcccg cgagttgacc
     1561 cgcatcctgc gcgcggtctg cgccggggag agctacgtga cgcctgccct ggccgccagc
     1621 ctgctgcgcg agatgaccgg ggtggcgccg ggcgcgcgcc cggccgtgga tccgctgaac
     1681 gaactgaccg accgcgagcg ccagatcctg gaaggcgttg ccaacggcct gagcaacaaa
     1741 gagatcggtc agcaacttca cctgaccgaa aaaaccgtga agcactacat gaccaacatc
     1801 ctgcagaagc tgcaggtgcg caaccgggtg gaagcggcgc tcattgcccg ccgcggcgct
     1861 cccccgcgct agccccaccc gcgccacctt cccccgcgct acttgcgtcc cgcgcccgca
     1921 gcgggtatga tgaagtagcg catcagcccg ccggcctgcc gttggcaccc caggccctgg
     1981 gcggcccatc cacatgaatt agaaagcagt acccccgatg gacgcgttcc tcctgtcgcc
     2041 ctccgccatc agaaacctgc cgatcctgat cctgaacctg ctgctggcga tctatctgct
     2101 gcgcggctac cgccgctcgc gcgcgaccgc gctgctggcg ctctggatgt gcagcctggc
     2161 cgtcgccttc ctggcgatcc tgctgtcgcg cacggtctac gacaacccgg tgctgagctt
     2221 catcgcgaac tggcaggtgg cgacgctggc cgggctggcc ggcaccgttg tgttgctggc
     2281 gttcgcctat gccttcccgc gcaaccccta tccgcgcgag gcgcgcgccg tgctggccgt
     2341 cgcgggcgtg gtcgccgccg ggctcggcgt gctgctgctg gccggcatcc ccgccggccc
     2401 ggcgagctac tggtacgagt tccgccgctt tgtctggacg ccgcacgagc tggggctggg
     2461 caacgtcctc tatccgctgg tgctgccggt gggctacc
//
LOCUS       DASUYC010000589         2177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_315818,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000589 DASUYC010000000
VERSION     DASUYC010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315818"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..1546)
                     /gene="crtI"
                     /locus_tag="VF276_09600"
     CDS             complement(44..1546)
                     /gene="crtI"
                     /locus_tag="VF276_09600"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HEX6607154.1"
                     /translation="MGQPRVAVVGAGVGGLATAARLAAAGYGVTVCERDAQPGGKMTR
                     VQAQGFSFDTGPSLITMPGVPAETFRAAGRRLGDYLDLRQLDPICRYFYPDGVRLDTS
                     SNLAAMTAAIGRLAPGDVGGYLDFLAHSRKLYERAALPFLYGERPRPRDLLARRGLDV
                     LRIDALTTMDGVVRRYFRDPHLVQLFDRYATYNGSSPYRAPGTLCLIPYVELVGGGWY
                     IQGGLYRLVESLVAVCCELGVDLRLGTAVEEVLIAGAGRGARATGLRLAGGATVPADA
                     VVVNADPLYARDALFAPRHRTAVGGRPVSPDADLSCSGFVLLLGTARRWPQLAHHNIF
                     FSAGYPAEFRAIFDARRPAPDPTIYISYTSASDESQAPPGHGNLFVLVNAPPVADPAR
                     WAEWAGPYAERILDRLEGAGLAGLRESIVYRQTLTPADFAARFNAYGGALYGYASHSK
                     GAAFARPANRAPGVRGLYFAGGSAHPGGGVPLVLLGARLVARLVQEDTCG"
     gene            complement(1550..>2177)
                     /locus_tag="VF276_09605"
     CDS             complement(1550..>2177)
                     /locus_tag="VF276_09605"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class F sortase"
                     /protein_id="HEX6607155.1"
                     /translation="GQPQEVLIQAFERRVLTYQPSAAAQWRVQMGNIGLHYYKWRYGA
                     TWYDGVTGPAPRPAAEPAMPHQLVIPKIGVRANIESLGVDQNNNFDIPKDPWNVGWYA
                     PGTRPGAVGSAAIDGHLDWYNVGPVVFWRLGDLKAGDRYWVRDSKGRDFGFVATEAST
                     CPYDNCPTQRIFGATDGRHLNLITCHGKFDRSARNYDKRLVVYGDLMQ"
BASE COUNT          330 a          759 c          795 g          293 t
ORIGIN      
        1 gagttgcaag gcgagcgggt gcttgccggc tttgatcaac gagtcatcca cacgtatcct
       61 cctgcaccag ccgggcgacc aggcgcgcgc ccagcaaaac cagcggcacg cccccgccgg
      121 gatgcgcgct gccgccggca aaatagaggc cgcgcacgcc gggcgcgcgg ttggcgggcc
      181 gggcgaacgc cgcccctttg ctgtgcgacg catagccata gagcgcgccg ccgtaggcat
      241 tgaagcgggc ggcgaaatcg gcgggcgtga gcgtctgccg gtagacgatg gactcgcgca
      301 ggccggccag ccccgcccct tccaggcggt ccaggatgcg ctcggcatac ggcccggccc
      361 actccgccca gcgcgccggg tcggccacgg gcggcgcgtt gaccagcacg aacagattgc
      421 cgtggcccgg cggggcctgg ctctcgtcac tcgcgctggt gtagctgata tagatcgtcg
      481 ggtcgggcgc ggggcggcgc gcgtcgaaga tggcgcggaa ctccgccggg tagccggcgc
      541 tgaagaagat gttgtggtgg gccagttgcg gccagcgccg tgccgtgccc agcagcagca
      601 cgaaccccga gcaggacagg tcggcatcgg gcgaaaccgg gcgacccccc accgcggtcc
      661 ggtggcgcgg ggcgaacagg gcatcccgcg catacagcgg gtcggcgttc accaccaccg
      721 catccgcggg caccgtggcc ccgcccgcca gccgcaggcc ggtcgcccgc gcgccccgcc
      781 ccgccccggc gatcagcact tcctccaccg ccgtgccgag gcgcagatcc acgccgagtt
      841 cgcagcagac cgcgaccagg ctctcgacca gccggtagag gccgccctgg atataccaac
      901 cgccgcccac cagttcgacg taggggatca ggcagagcgt gcccggcgcc cggtagggcg
      961 aagagccgtt gtaggtggcg tagcggtcga aaagctggac caggtgcggg tcgcggaaat
     1021 agcggcggac gacgccgtcc atcgtcgtca aggcgtcgat gcgcagcacg tcgaggcccc
     1081 ggcgggccag caggtcgcgc gggcggggcc gttcgccgta gaggaagggc agcgccgccc
     1141 gctcgtacag cttgcggctg tgggccagga agtcgagata gccgcccacg tcgcccggcg
     1201 ccaggcgccc gatggccgcg gtcatggcgg cgaggttgct gctcgtgtcg aggcgcacgc
     1261 cgtcgggata gaagtagcgg cagatggggt cgagttgccg cagatcgaga tagtcgccca
     1321 ggcggcggcc cgcggcgcgg aacgtctccg ccggcacgcc gggcatggtg atcagcgagg
     1381 ggccggtgtc gaagctgaag ccctgggcct gcacgcgggt catcttgcca ccaggctggg
     1441 cgtcccgttc gcaaacggtg acgccgtagc ccgcagcggc caggcgggcg gccgtcgcca
     1501 gcccgcccac gcccgcgccg acgaccgcga cccgcggctg acccatgccc tactgcatca
     1561 ggtcgccgta gacgaccagg cgcttgtcgt agttgcgggc cgagcggtcg aacttgccgt
     1621 ggcaggtgat caggttgaga tggcgcccgt cagtcgcgcc gaagatgcgc tgcgtggggc
     1681 agttgtcata cgggcaggtc gaggcctcgg tcgccacgaa gccgaagtcg cggcccttgc
     1741 tgtcgcgcac ccagtagcgg tcgcccgctt tcaggtcgcc cagccgccag aagaccaccg
     1801 ggccgacatt gtaccagtcg agatggccgt cgatggccgc gctgcccacc gcgcccggtc
     1861 gggtgcccgg cgcgtaccag ccgacgttcc aggggtcctt ggggatgtcg aagttgttgt
     1921 tctgatccac gcccagcgac tcgatattcg cccgcacgcc gatcttgggg atgaccagtt
     1981 ggtggggcat ggccggctcg gcggcggggc ggggcgcggg gccggtcacg ccgtcgtacc
     2041 acgtcgcgcc atagcgccat ttgtagtagt gcaggccgat gttgcccatc tgcacgcgcc
     2101 actgtgcagc tgcgctcggc tgataggtca gcacccggcg ctcgaacgcc tggatcaaca
     2161 cctcctgcgg ctgaccg
//
LOCUS       DASUYC010000590         3098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_266653,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000590 DASUYC010000000
VERSION     DASUYC010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266653"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..1014
                     /locus_tag="VF276_09610"
     CDS             127..1014
                     /locus_tag="VF276_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX6607156.1"
                     /translation="MALSAWANRNIKWLFILPAAIFVAVMMIFPIIYTGGLSFTQWSG
                     SATQPAQWVGLDNYTTLLFNDQRFRDAFVRTFIFTIGAVVIELILGLAIALLLRREFR
                     GQNVVKTLILLPMVATPVAMGMAWLLMLEPTIGFANYALQRLGLPPQQWLASTDQALP
                     SLMLVDIWEWTPMIALIALAGLATLPDEPFEAATVDGAGPWQRFLHLTLPLLAPTLVV
                     ATLLRAIDALKTFDIIYTMTQGGPGYSTETLNIYSYVQGFGYFQLGMASSLLVIFFAI
                     VLGVSLLFAGVRNRWGAAT"
     gene            1011..1940
                     /locus_tag="VF276_09615"
     CDS             1011..1940
                     /locus_tag="VF276_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010281225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX6607157.1"
                     /translation="MSNTTHDQTVAARDAARAAGRPAVAPPEAIVVPGAEAAHRRALI
                     GNIIRYIVIVLVALAFTFPFIWMLLASFKTQADIMNNQNILSFTPTFQHYIDVFNQYS
                     FFKFMGNSLLVALVSTAFSLVLGLPAAYAIARYKMPAFGLILLAARIVPGITFLIPWF
                     ILFTQVHLVGTYWALILSHMLVGLPFITWVMISFFEGLPHELEEAAQVDGVTPIGSFF
                     RIALPLSLPGIITGSILSFIFSWNNFMFSLVLASQETRTLPVAIFSFISYASIDWGGL
                     MAAAVIITLPVLIITLFVQRYVVSGLTAGALKG"
     gene            1950..2999
                     /locus_tag="VF276_09620"
     CDS             1950..2999
                     /locus_tag="VF276_09620"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX6607158.1"
                     /translation="MQALVKYANEPGKVELRDMPRPTITRDQVLLRVRAVGICGSDLE
                     MYHHKITFPVNPPVILGHEFSGTVEEAGADVTDFAPGDRVVSETAAYVCGKCAECRTG
                     NYNECPNRLGFGVLINGADAPYVAVRQGILHHVPDNVDLAEAALTEPCCVAYNALVVK
                     SRIRPADVVVVLGPGPIGLMATQIARVSGASAIVLAGLAQDRARMEIGQEVGATHLVD
                     LSKDDLPALVQDLSGGRGADLVVDAAGPQATVKLAMDLVRRNGQITKIAWGPKPLELS
                     LDPLLAKAVTLQGVFSHTWDTWERVLRLESTRQVNLGALISHRVPLADWKVAFDALES
                     GAAVKALIFPNGVKE"
     gene            3001..>3098
                     /locus_tag="VF276_09625"
     CDS             3001..>3098
                     /locus_tag="VF276_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007333778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabino 3-hexulose 6-phosphate aldehyde lyase"
                     /protein_id="HEX6607159.1"
                     /translation="MQPIVQLSLDLTSIDEALHVAEIGVQAGVDWL"
BASE COUNT          484 a         1047 c         1007 g          560 t
ORIGIN      
        1 cagttcaacg acttcctgaa gaccgacctg cagaagaaat aagcgcgggg cacggacggc
       61 gcgggggcgc gatccctgcc gcgccgtccg cgccataccc ggctcgacgg gaaaggaggt
      121 ggcgcgatgg cgctctcagc ctgggccaac cgcaacatca agtggctgtt tatcctgccc
      181 gcggcgatct tcgtggccgt gatgatgatc ttcccgatca tctacaccgg cgggctgagc
      241 ttcacccagt ggagcggctc ggctacccag cccgcgcaat gggtcggcct cgacaactac
      301 accacgctgc tgttcaacga tcaacgcttc cgcgacgcct tcgtgcgcac gttcatcttc
      361 accatcggcg cggtcgtcat cgaactgatc ctcgggttgg cgatcgcgct gctgctgcgc
      421 cgcgagttcc gcgggcagaa cgtggtgaag acgctgatct tgctgcccat ggtcgctacg
      481 ccggtggcga tgggcatggc ctggctgctc atgctggagc cgaccatcgg cttcgccaac
      541 tacgcgttgc agcgcctggg cctgccgccc cagcaatggc tcgcctccac cgaccaggcg
      601 ctgccctcgc tgatgctggt cgatatctgg gagtggacgc cgatgatcgc gctgatcgcc
      661 ctggccgggc tggcgacgct gccggacgag ccgttcgagg cggccacggt ggacggggcg
      721 ggcccctggc agcgcttcct ccacctcacc ctgccgctgc tggcgcccac cctcgtcgtg
      781 gcgacgctgc tgcgggccat cgacgccctc aagaccttcg acatcatcta caccatgacc
      841 cagggcgggc cgggctactc caccgagacg ctgaacatct actcgtatgt ccagggcttc
      901 ggctacttcc agctaggcat ggcgtcgtcg ttgctggtga tcttcttcgc catcgtgctg
      961 ggggtcagcc tgctgttcgc gggcgtgcgt aaccgctggg gggccgcgac atgagcaaca
     1021 ccacccatga ccagaccgtg gccgcacgag acgcagcgcg cgccgcgggc cggcccgcag
     1081 tcgccccgcc cgaggccatc gtcgtgcccg gcgcggaagc cgcgcaccgg cgcgcactca
     1141 tcggcaacat catccgctac atcgtcatcg tgctggtggc gctggccttt accttcccgt
     1201 tcatctggat gttgctggcc tcgttcaaga cgcaggccga cattatgaac aaccagaaca
     1261 tcctgagctt cacgccgacg ttccagcact acattgacgt gttcaatcag tactcgttct
     1321 tcaagttcat gggcaacagc ctgctggtgg cccttgtctc gacggcgttc tcgctggtgc
     1381 tgggactgcc cgccgcctac gctatcgcgc gctacaagat gccggccttc gggctgatcc
     1441 tgctggccgc acgtatcgtg cccggtatca ctttcctgat cccgtggttc atcctgttta
     1501 cccaggttca cctggtcggc acctactggg cgctgatcct cagccacatg ctggtggggc
     1561 tgccgttcat cacctgggtg atgatctcgt tcttcgaggg gctgccgcat gaactggaag
     1621 aggcggccca ggtcgacggc gtcacgccca tcgggtcgtt cttccgcatc gcgctgccgc
     1681 tgtcgctgcc ggggatcatc accgggtcga tcctctcgtt tatcttttcc tggaacaact
     1741 tcatgttttc gctggtgctg gccagccagg aaacgcgcac gctgccggtg gcgatcttca
     1801 gcttcatctc ctacgcctcg attgactggg gcgggctgat ggccgccgcg gtgatcatca
     1861 ccctgccggt gctgatcatc accctgtttg tgcagcgcta cgtggtcagc ggcctgacgg
     1921 ccggcgcgct gaagggatag tcacctcata tgcaagcttt agtgaaatac gcgaacgaac
     1981 ccggcaaggt cgaactgcgc gacatgccgc gcccgacgat cacgcgggac caggtgttgc
     2041 tgcgggtgcg cgccgtgggc atctgcggca gcgacctgga gatgtatcac cataagatca
     2101 cgttcccggt gaacccgccg gtgatcctgg gccacgagtt ctccggcacg gtcgaggagg
     2161 ccggcgccga tgtgaccgac ttcgcgccgg gcgaccgggt ggtcagcgag acggcggcct
     2221 atgtctgcgg caaatgcgcc gagtgccgca cgggcaacta caacgagtgc cccaaccggc
     2281 tcggcttcgg cgtgctgatc aacggcgccg acgctcctta tgtggccgtg cgccagggca
     2341 tcctgcacca tgtgcccgac aacgtggacc tggccgaagc ggcactgacc gagccgtgct
     2401 gtgtggccta caacgcgctg gtggtcaaga gccgcatccg ccccgccgac gtggtcgtgg
     2461 tgctcggccc cggccccatc gggctgatgg cgacgcagat cgcccgcgtc agcggcgcct
     2521 cggccatcgt gctggccggg ctggcccagg accgggcgcg catggagatc ggccaggagg
     2581 tcggcgcgac ccacctggtc gatctgagca aggacgacct gcccgcgctg gtgcaggacc
     2641 tgtcgggcgg gcgcggcgcc gacctggtgg tggacgccgc ggggccgcag gccacggtca
     2701 agctggcgat ggacctcgtg cgccgcaacg gccagatcac caagatcgcc tgggggccga
     2761 agccgctgga gttgagcctc gacccgctgc tggcgaaggc ggtgacgctg caaggcgtgt
     2821 tcagccacac ctgggacacc tgggagcgcg tgctgcgcct ggagtccacc cgccaggtca
     2881 acctgggcgc gctgatcagc caccgggtgc ccctggcaga ctggaaggta gccttcgacg
     2941 cgctggagag cggcgcggcg gtcaaggcgc tcatcttccc caacggggtc aaggagtagc
     3001 atgcaaccca tcgtccaact gtcgctggac ctgacttcga ttgacgaagc gctgcacgtg
     3061 gccgagatcg gcgtgcaggc gggcgtggac tggctcga
//
LOCUS       DASUYC010000591         2712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_16077,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000591 DASUYC010000000
VERSION     DASUYC010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16077"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1749
                     /locus_tag="VF276_09630"
     CDS             <1..1749
                     /locus_tag="VF276_09630"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6607160.1"
                     /translation="GVMEFLSRVPGGRDPDLPAVTAALGSQIGQFIGRTQVEEDLRRS
                     RDQLAIILRGVADGITMTTPTGKLLYANEAAAQMMGYPTPEAALAAPPGEALSKFEIL
                     DAEGRPFPIEHLPSRQAFTGADHPGLILRFRVRATGEERWSVVRATAVRDPAGQVVSA
                     ISIFRDMTESRRTAEAARFLAGAGALLAASLDFETTLDNVAHLAVPALADWCYVDILE
                     DDGTIRRLAAAHADPAKEALTAELQRFPPAPGSAHPVVEVLRTGRPLLWTNPPAALPA
                     QIARDAEHQALIERLGAGSFMVVPLRARGRILGAITFVTSDPARGFDAATQRLAEDLA
                     ERAAVAVDNARLYRAAQEAVRARDEFLSVAAHELRTPITSLRGYAQLIVRQFERTGPP
                     DPDRVRRALQVIDQQSAKLAHLVGQLLDSARIEAGRLALERAPADVADLAASLVRATQ
                     ARTQQHTIRLDAPPALPATVDALRLEQVLTNLLDNAVKYSPDGGPVVVALTTPDPAWL
                     QITVTDQGIGIPPAQRERIFERFYQGHGPGSFGGMGLGLHISRQIVELHGGQITVEAP
                     PAGGTRFVVRIPLPGP"
     gene            1941..>2712
                     /locus_tag="VF276_09635"
     CDS             1941..>2712
                     /locus_tag="VF276_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX6607161.1"
                     /translation="MRCLVTGAAGFIGSHLCEALVGGGAEVVGLDAFIPYYPRTVKER
                     NLAALRTNPRFTFVERDLRTDDLAPALEGVDTVFHLAAMGGLLLSWTNFDLYMTCNIQ
                     ATQRLLEAARVAGGVHRFIHASTSSIYGSEVTGPETTTPHPVSPYGITKLAAENLVQT
                     YDRQFGIPATILRYFSVFGPRQRPDMGYYLFIDKILHGEPITVFGDGTQLRGNTYVGD
                     IVRATLLAHERFTRGAVYNVGGSEEISANQVIGILEELI"
BASE COUNT          391 a         1015 c          918 g          388 t
ORIGIN      
        1 ggcgtgatgg agtttctcag ccgggtgccg ggcgggcgcg accccgacct gccggcggtg
       61 acggcggccc tcggcagcca gatcggccag ttcatcggcc gcacccaggt ggaggaagac
      121 ctgcgccggt cgcgcgacca actggcgatc atcctgcgcg gcgtggccga cggcatcacc
      181 atgaccaccc cgacgggcaa gttgctgtat gccaacgagg ccgccgcgca gatgatgggc
      241 tatcccacac ccgaagcggc cctggccgcg ccccccggcg aggcactttc caagttcgag
      301 atcctcgacg ccgaaggccg gcccttcccg atcgagcact tgccgtcgcg gcaagctttt
      361 acgggcgccg accatccggg cctgatcctg cgcttccgcg tgcgcgccac cggcgaggag
      421 cgctggtcgg tcgtgcgcgc caccgccgtg cgcgacccgg cgggccaggt ggtctcggcc
      481 atcagcatct tccgcgatat gaccgagagc cgccgcaccg ccgaggccgc gcggttcctg
      541 gccggcgccg gcgccctgct ggccgcctcg ctcgactttg agaccacgct cgacaacgtg
      601 gcccacctgg ccgtgcccgc gctggccgac tggtgctatg tggacatcct ggaggacgac
      661 ggcaccatcc gccgcctggc cgcggcccac gccgacccgg cgaaggaagc cctcaccgcc
      721 gagttgcaac gcttcccgcc cgcgccgggc agcgcgcacc cggtcgtcga ggtgctacgc
      781 accgggcgcc cgctgctgtg gacgaacccg cccgccgcgc tgcccgcgca gatcgcccgc
      841 gacgccgagc accaggccct gatcgagcgc ctgggtgccg gcagcttcat ggtcgtgccc
      901 ttacgcgccc gcgggcgcat cctgggcgcg attactttcg tcacgtcaga cccggcgcgc
      961 ggcttcgacg ccgccaccca gcgcctggcc gaggatctgg ccgagcgtgc tgccgtcgcc
     1021 gtcgataacg cccgcctcta ccgcgccgcg caggaggccg tgcgcgcccg cgacgagttt
     1081 ctctcggtgg ccgcgcacga gctgcgcacg cccatcacca gcctgcgcgg ctacgcgcaa
     1141 ctgatcgtgc gccagttcga gcgcaccggg ccgcccgacc ccgaccgcgt gcgccgcgcg
     1201 ctgcaggtca tcgaccagca gtcggccaag ctggcccacc tggtcggcca gttgctcgac
     1261 agcgcgcgca tcgaggcggg gcggctggcc ctggagcgtg cgcccgccga cgtggccgac
     1321 ctggcggcca gcctggtccg cgccacgcag gcgcgcaccc agcagcacac catccgcctg
     1381 gacgcgccgc ccgcgctgcc ggccaccgtg gacgccctgc gcctggagca ggtgctgacc
     1441 aatctgctcg acaacgcggt caagtacagc cccgacggcg gcccggtggt ggtggcgctg
     1501 accacgccgg atcccgcctg gttgcagatc accgtgacgg accagggcat cggcatcccg
     1561 cccgcgcagc gcgagcgcat cttcgagcgc ttctaccagg ggcacggccc cggctcgttc
     1621 ggcggcatgg gcctgggcct gcacatcagc cgccagatcg tcgagttgca cggcggacag
     1681 atcacggttg aggcgccccc ggcgggcggc acgcgcttcg tcgtgcgcat cccgttgccc
     1741 ggcccctaaa ggggcgggca acatgtacga agcccgccgc cgcgggctga gaccggggcg
     1801 gcgcgttcat gatattatat gggaactcat tggtaggccg cgcaggcggc ctttgttaac
     1861 gtagcccgcc cctttagggg ccgggcggcg ccgcgcctgg aggccggtcc ctgcggcgac
     1921 cgggaaaaag gagcctgttt gtgcgttgtc tggtgacggg ggccgccggg tttatcggct
     1981 cccatttgtg cgaggccctg gtgggcggcg gcgccgaggt ggtcgggctg gacgccttca
     2041 tcccctacta tccgcgcacg gtgaaggagc gtaacctggc cgcactgcgg accaatcccc
     2101 gcttcacctt cgtcgagcgc gacctgcgca ccgacgacct ggcccccgcc ctggaggggg
     2161 tggacaccgt gttccacctg gcggcgatgg gcggcctctt gctgagctgg accaacttcg
     2221 acctctacat gacctgcaac atccaggcga cgcaacgcct gctcgaagcg gcgcgcgtcg
     2281 cgggcggcgt ccaccgcttc atccacgcca gcacgtcgag catctacggc agcgaagtca
     2341 ccgggccgga gacgaccacg ccgcacccgg tctcgcccta cggcatcacc aagctggccg
     2401 cggagaatct ggtgcagacc tatgaccggc agttcggcat cccggccaca atcctgcgct
     2461 acttctcggt cttcggcccg cgccagcgcc ccgacatggg ttattacctg ttcatcgaca
     2521 agatcctgca cggcgaaccg atcacggtgt tcggcgacgg cacccagttg cgcggcaaca
     2581 cctacgtggg cgacatcgtg cgagccacgc tgctggcgca cgagcgcttc acgcgcggcg
     2641 cggtgtacaa cgtgggcggg tcggaggaga tcagcgccaa ccaggtgatc ggcatcctgg
     2701 aggagttgat cg
//
LOCUS       DASUYC010000592         2601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_96715,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000592 DASUYC010000000
VERSION     DASUYC010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96715"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..740)
                     /locus_tag="VF276_09640"
     CDS             complement(90..740)
                     /locus_tag="VF276_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607162.1"
                     /translation="MHKHHHHHHDEEWDAGDARYGGPEGDPGWGPPPGYGWGGRPFPP
                     PGAFHHGGGFGRGFGFGPWMFHRGFGPWMFHGRRGPFGRGFGPGWGGPGPGFRGWGGP
                     GWHGPGPGFRGWGGPGWHGPGPGFHGGHGPGPDDEGDWRGPQFRGGWGHHGHRGHHGR
                     HGGPDVAWQIARLEQAQAFLEQRLTDVKTALEHLRAQQQQQDQPPPGGTDRPTVHL"
     gene            complement(778..1167)
                     /locus_tag="VF276_09645"
     CDS             complement(778..1167)
                     /locus_tag="VF276_09645"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX6607163.1"
                     /translation="MEPPIWGFLQPCLLLLLAEGPAHGYSLMEELNRRQLLPGDVDVG
                     NLYRALRRMEAADLVTSAWSTHERGPNKRVYQITDRGEEVLRHWAGALEERTRLLRAF
                     LDEYHRVFGQPPTVAPPPRPEDQDNFM"
     gene            complement(1570..>2601)
                     /locus_tag="VF276_09650"
     CDS             complement(1570..>2601)
                     /locus_tag="VF276_09650"
                     /inference="COORDINATES: protein motif:HMM:NF027641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607164.1"
                     /translation="PTRTPTRTPTPAAGTPTAVPTRTNTPVPPAGTPPPVATATATPG
                     GVPDSLALPIRAAFVYPWFPEAWTQHGIYPYTRYTPTLGYYNQNDPAILTRQIAAMQY
                     GNIQAGIASWWGQGTPTDGRIPALLAAAHSTSFRWALYYEAEGSGNPSVAQIQADLSY
                     IQTRYMRDPNYLWVNGRPVIFVYGDGSDSCAMATRWAQANTIGAYVVLKVFSGYRTCA
                     DQPASWHQYGPAVATDSQAGYSFAISPGFYKADEAAPRLVRDLSRWQQNIRSMIASQA
                     PWQLITTFNEWGEGTSVESSTGWASPSGWGAYLDALHSNGGLAMATNNPPEAAAQPAT
                     SRDKEKRHR"
BASE COUNT          370 a          868 c          940 g          423 t
ORIGIN      
        1 gcattgaggc gcaaaaagag gccctggaca ccgtccaggg tctcggtgcg cgtggggcgg
       61 gcgcttgtcg gcgcccgccg ggttgttggc tagagatgga ccgtgggccg gtcggtgccg
      121 ccggggggcg gctggtcctg ctgttgctgc tgggcgcgca ggtgctccag cgcggtcttc
      181 acgtcggtca gccgctgctc caggaacgcc tgggcctgct ccagccgggc gatctgccag
      241 gcgacatcgg gtccgccgtg ccggccatgg tggccgcgat gaccgtggtg gccccagccg
      301 ccgcggaact ggggtccgcg ccagtcgcct tcgtcgtccg gtcccggtcc gtgcccgcca
      361 tggaagccgg gtcccggccc atgccagccc ggcccgcccc agccgcggaa gcccggcccc
      421 ggcccatgcc agcccggccc gccccagccg cggaagccgg gacccggccc gccccagccc
      481 ggcccaaagc cgcgcccgaa cgggccgcgt ctaccgtgga acatccacgg gccgaatccg
      541 cggtggaaca tccacgggcc gaagccaaag ccgcgtccga agcccccgcc gtgatggaag
      601 gcgccgggcg gggggaaagg ccgcccgccc cagccgtagc cgggcggcgg cccccagccg
      661 gggtcgccct ccggcccgcc atagcgcgcg tcaccggcgt cccattcctc gtcatggtgg
      721 tgatgatgat gcttgtgcat actgctaacc ttcccctttc agtagcccat cctggtgcta
      781 catgaagttg tcctgatcct ctggacgtgg tgggggtgcg acggtgggcg gctgcccgaa
      841 gacgcggtgg tactcgtcga ggaaggcgcg cagcaggcgg gtgcgctctt ccagcgcgcc
      901 ggcccagtgg cgcagcactt cctcgccccg gtcggtgatc tggtacaccc gtttgttcgg
      961 tccccgttcg tgcgtgctcc aggccgaggt gacgagatcg gcggcctcca tgcgccgcag
     1021 ggcgcggtag agattgccga catcgacatc gcccggcagg agttggcgcc ggttgagttc
     1081 ctccatcagg ctgtatccgt gggccgggcc ttcggccagc aggagcagca ggcacggttg
     1141 caggaacccc cagatgggcg gttccatgaa cgagcccccg ccatgtctcc cgtggccgaa
     1201 cggcccccca aatccgtgat gcacgccgga tcccacctct tcacgcagta caatctaccc
     1261 tcagtgtccg tagatatatg ttaattacat atatcttgca ccaatattac atccgatacg
     1321 gcgcgctgtc aagaggggag accgaaatcg attcaaatcc gctggttctg caccgcgacg
     1381 ggcgcgaaga atgcgaagaa caaaacttac acagagtgtc ttcgcgtctt cgcgccggcc
     1441 ttatgaaagg atgcgcagta ggcggagggc caccggcgcc gcgcaaggca gaggccgggc
     1501 gggggatgca tccctccggc ccggcctccg ttgccgtatc cgccctagcc tccggcgggc
     1561 gggcgcggct tagcggtgcc gcttttcctt gtcacgtgat gtcgcgggct gggcggcggc
     1621 ctccggcggg ttgttggtcg ccattgccag gccgccgttg ctgtgcagcg cgtccaggta
     1681 cgcgccccag ccgctcggcg acgcccagcc ggtgctcgac tccacgctgg tgccctcgcc
     1741 ccactcgttg aacgtggtga tcagttgcca gggcgcctgc gaggcgatca tgctgcggat
     1801 attctgctgc cagcggctca ggtcgcgcac gagccggggc gcggcttcat cggccttgta
     1861 gaaccccgga ctgatggcga aactgtagcc ggcctgcgaa tccgtggcga cagccgggcc
     1921 gtactggtgc cagcttgccg gctggtcggc gcaggtgcgg tagccgctga agaccttgag
     1981 caccacatag gcgccgatgg tgttggcctg ggcccagcgg gtagccatcg cgcaactatc
     2041 actgccgtcg ccgtagacga agatgaccgg gcgcccgttg acccacagat agttgggatc
     2101 gcgcatgtag cgcgtctgga tatagctcag gtcggcctgg atctgggcga cggagggatt
     2161 gccgctgccc tcggcctcgt aatagagcgc ccagcggaag gaagtggagt gcgccgccgc
     2221 gagcagggcc gggatgcgcc cgtccgtcgg ggtgccctgg ccccaccagg aggcgatccc
     2281 ggcctggatg ttgccgtact gcatggcggc gatctgccgg gtcaggatgg ccggatcatt
     2341 ctggttgtaa tagcccaggg tcggcgtgta gcgggtgtag ggatagatgc cgtgctgggt
     2401 ccaggcttcg gggaaccagg ggtacacgaa cgccgcgcgg atcggcagcg ccaggctgtc
     2461 cggcacgccg cccggcgtgg cggtggccgt cgccacgggc ggcggcgtgc cggcgggggg
     2521 cacgggcgta ttggtgcgcg tcggcaccgc ggtcggcgtg ccggcggcgg gcgtgggtgt
     2581 gcgggtgggc gtgcgggtgg g
//
LOCUS       DASUYC010000593         2557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1282549, whole genome shotgun sequence.
ACCESSION   DASUYC010000593 DASUYC010000000
VERSION     DASUYC010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282549"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VF276_09655"
     CDS             complement(<1..483)
                     /locus_tag="VF276_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607165.1"
                     /translation="MQAPPKRWKKRQQLKPDVIAELQAALADLKLTPLLLQLLANRDI
                     INPQALHAEPEDDHTALPPALTVEGMHAAAAAFLDPVHTPLPSPLTIKGMAAAVDRIV
                     QAIQGKETIAVYGDYDADGVTSTVLLTQALRAWGANVIIRVPHRKREGYGLNRAALDE
                     L"
     gene            complement(538..1485)
                     /locus_tag="VF276_09660"
     CDS             complement(538..1485)
                     /locus_tag="VF276_09660"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX6607166.1"
                     /translation="MAVHAHPDDEVVFTGGILPVYHDRGVRTVLVCCTGGEEGEIHDP
                     DLDPDEARPRLGAIRAGELACAVQAMDIDVLEMLGYRDSGMAGTEANANPACFHQAPR
                     DEATAKLVALVRRYQPQVLVTYNDFGAYGHPDHIAAHQITMAAYDLAADPAYRPDLGA
                     PWAPLKLYYTAWPEESFEKARAMYLERGLKWPWDEDEKPADADPEHQSDEEGVSAEVA
                     AEAKPSEPEKPKYEPPPVTTRVDVRAGVARKMQAFLCHRTQFMHEGLFMTMPEDIAPV
                     AFGEDDFTLVRSRIAAPTPETDLFAGITAPALQPADQMC"
     gene            complement(1595..>2557)
                     /locus_tag="VF276_09665"
     CDS             complement(1595..>2557)
                     /locus_tag="VF276_09665"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6607167.1"
                     /translation="LVGIATAPSLGIALAWTFLLGLGWGCQDVTLNVFVADLYPENRN
                     AALNLTNVFFGLGALVGPLAVGVALTTVASPQPVLWLLAGLAAVGLLLFLVLRFPPLR
                     AAPQGTSTGRRALGVLREGYVLLTALMFFLYVGLEIGFGGWAYSFALEGARLEAGVAA
                     LVVSVYWIAFTVGRAAAGVASRRWPGAELVWAGALCGAVGAAVLALFPASPVVLFGSA
                     VLLGVGFAPIFPTAFGLAAARYPAVAGTVSSVLVLGGSLGGTVLPYAQGRLLTAGGVP
                     LAAGAIALCALAVAGLQLFLGRVYQAPPASEAAPPAEPAPCVAA"
BASE COUNT          385 a          880 c          921 g          371 t
ORIGIN      
        1 gagttcatcc agggcggccc ggttcaaccc gtagccctcg cgcttgcggt gcggcacgcg
       61 gatgatgacg ttggcgcccc aggcccgcag cgcctgggtc agcagcaccg tcgaagtcac
      121 cccatcggcg tcgtagtcgc cgtagacggc gatggtctcc ttgccctgga tggcctgcac
      181 gatgcggtcc accgccgcgg ccatgccctt gatcgtgagc gggctgggca ggggcgtgtg
      241 gaccgggtcg aggaaagcgg cggcggcggc atgcatcccc tcgacggtga gcgccggggg
      301 cagcgccgtg tgatcgtcct ccggctcggc gtggagcgcc tgcgggttga tgatgtcgcg
      361 gttggcgagg agttgcagca gcagcggcgt gagtttcagg tcggcaagcg ccgcctggag
      421 ctcggcaatg acatcgggct tcagttgctg gcgcttcttc caccgtttgg gtggtgcttg
      481 cacgaggcgg atctccttcg cgggcctaat gtggcaggcg gaatgttccg ctgaatatta
      541 gcacatttgg tcggcgggct gcaaggcagg cgccgtgatg cccgcgaaga gatccgtttc
      601 cggggtgggc gcggcgatcc ggctgcgcac cagcgtgaag tcgtcctcgc cgaaggccac
      661 cggagcgatg tcctccggca tcgtcatgaa cagcccctcg tgcatgaact gggtgcggtg
      721 gcaaaggaaa gcctgcatct tgcgcgcgac ccccgcgcgc acatccacgc gcgtcgtgac
      781 cggcggcggc tcatacttcg gcttttccgg ctcggagggt ttagcttccg cggctacttc
      841 cgcgctcacg ccttcttcgt cgctctggtg ctcggggtcg gcatccgcgg gcttctcgtc
      901 ctcgtcccac ggccacttaa ggccgcgctc caggtacatg gcgcgggcct tctcgaacga
      961 ctcttcgggc caggccgtgt agtagagttt gagcggcgcc cagggcgcac ccaggtcggg
     1021 ccggtaggcc gggtcggcgg ccaggtcgta ggcggccatg gtgatctggt gcgcggcgat
     1081 gtggtcgggg tggccgtagg cgccgaagtc gttgtacgtc accagcacct gcggctggta
     1141 gcggcgcacc agggccacca gtttggccgt cgcctcgtca cggggcgcct ggtggaagca
     1201 ggccgggttg gcgttggcct cggtgcccgc catgccggag tcgcggtagc caagcatctc
     1261 aagcacgtcg atgtccatgg cctggaccgc gcaggccagt tcgcccgcgc ggatcgcgcc
     1321 caggcggggc cgtgcctcgt ccgggtcgag atcgggatcg tggatttcgc cttcttcgcc
     1381 gccggtgcag cagaccagga cggtgcgcac gccgcggtcg tgatagacgg gcaggatgcc
     1441 gccggtaaag acgacctcgt cgtcggggtg cgcgtgcacc gccatcagcg tgagcggtgc
     1501 ggtttgggtg gtttcagggg caggtgtatt cataccgcga ttatatcaca ctcctgggat
     1561 tcggcgcgta gggcaggcca caaaatgggc ccgctcaggc ggcaacacag ggcgccggtt
     1621 cggcgggcgg cgccgcttcg cttgccgggg gggcctggta gacgcggccc aggaacagtt
     1681 gcaggccggc cacggccagc gcgcacaggg cgatggcgcc cgccgccagg ggcacaccgc
     1741 ccgcggtgag caggcggccc tgcgcgtagg gcaggaccgt gccgcccagg ctgccgccga
     1801 gcaccagcac gctcgacacc gtgcccgcca ccgccgggta gcgcgccgcc gccagcccga
     1861 acgccgtggg gaagatgggc gcgaacccca cccccagcag caccgcgctg ccgaacagca
     1921 cgaccgggct ggccgggaac agcgccagca ccgccgcccc taccgcgccg cagagcgccc
     1981 cggcccacac cagttcggcg cccggccagc gccggctggc gacgcccgcc gccgcccgcc
     2041 ccacggtgaa ggcgatccag tagaccgaga cgacgagcgc agcgacgccc gcctccagcc
     2101 gggcgccttc gagggcgaag ctgtaggccc agccgccgaa cccgatttcc aggcccacat
     2161 agaggaagaa cattagcgcg gtcagcagca cgtagccctc gcgtaacacg cccagggcac
     2221 ggcggcccgt gctcgtgcct tgcggcgcgg cgcgcagcgg cgggaagcgc agcacgagga
     2281 acagcagcag ccccaccgcg gccagccccg ccagcagcca cagcaccggc tgcgggctcg
     2341 ccaccgtggt cagcgccacg cctacggcca gcggccccac cagcgcgccg aggccgaaga
     2401 agacattggt caggttcagc gcggcgttgc ggttctccgg gtagagatca gccacgaaga
     2461 cattcagggt cacgtcctga cagccccagc cgaggcccag caggaacgtc caggccagcg
     2521 cgatgcccag gctgggcgcg gtcgcaatgc cgactag
//
LOCUS       DASUYC010000594         7064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_657541,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000594 DASUYC010000000
VERSION     DASUYC010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 7064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7064
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657541"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2166)
                     /locus_tag="VF276_09670"
     CDS             complement(<1..2166)
                     /locus_tag="VF276_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607168.1"
                     /translation="MNRLRSKGLMVLIVGAFLLAVAGFAMERAAAQNDSRYFPPTGHT
                     VSGRFLAYWDQHGGLAQQGYPLSEPMQEVSDTNGQPYMVQYFERAVFEWHPENAAPYD
                     VLLSLVGTFEYQKRYGTAGAPNQHASTDNARFFPETKHYVGGKFRAYWESHGGLDQQG
                     YPISDEFTERSPLDGKVYTVQYFQRAVFELHPENAGSPYEVLLSQLGTFRYHARYTPP
                     VIPPPAPGHAQSRPKGTGGYLVWTDSLATPDSAGTGNIMALDLEMGKLLTVTDAPGDQ
                     INVAVSGPNVVWEEHPQVCGTCPVAIAGKNLLTGGVFTVAAAGALRNIEPAIYGQTVV
                     WIERDTGTGNSRLLTRDIGSPNITVVATLPSPLAFGPPALSDDYIIWSEYSEGAPGTG
                     PPDTLRAYNRHTGTTQTVTQLSDGRGASYVIGDHRVFWADFELEYRNLDTGDQATITY
                     FAINLVLAGDTLVWNQPGGLGNGLDIWGMKLGDRQITALKILPGDQEFPVIAGDWLVW
                     GVPDGRGGGTIAADLLAHAFATAVPPGQLTPSPAVPSPTAIYPPPVPTNPAGSPTPGP
                     DIRLAASRVIGEPVAGGQYVFYLSDEGNPLNTATPQPPGPPHLALYAGDVEQRQISLI
                     KNIVTDIPLIATDGMTLVWLESTAQGNQSIHSYNLQTHTESTIITDAVRHEFGGVAVD
                     NGVLYYQDATTGHIGLYSHDLRTGTEALISSTGRLPVAAD"
     gene            complement(2739..4631)
                     /locus_tag="VF276_09675"
     CDS             complement(2739..4631)
                     /locus_tag="VF276_09675"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX6607169.1"
                     /translation="MTTQTAGRAATGPQTENWRALAQQLRVDSIRSSTAAGSGHPTSS
                     MSAADLMAVLMSKYLRYDFQDPQNSANDHLIFSKGHASPLLYAMYRAAGAVSLDEMLT
                     FRKFGSRLQGHPTPIIPWVDVATGSLGQGLPIGVGVALAGKRLDKIPYHVWVLLGDSE
                     MAEGSIWEAFDHAGHSGLNNLIGILDMNRLGQRGETELGWNSGAYAERARAFGWHAVE
                     IDGHDLAAIDAAYAEAEQQQNQPTLIVAKTMKGRGVSFLENKEGWHGKALDADQAKQA
                     IQELGGETNIQVQVQKPERQGAPPPQGDNAKVELPTYKVGDKVATRKAYGDALAALAA
                     RPDVVALDAEVSNSTHAEEFKKVAPDRFFEMYIAEQQMVAAAVGMSVRHRVAFASTFA
                     AFLSRAYDFIRMAAVSRANLRLCGSHAGVSIGEDGPSQMALEDLAMMRAVHGSTVLYP
                     SDPNQTAQLVGAMADRPGISFLRSTREKTAVLYPPDEQFTIGGSKVVRQSDKDQVTVV
                     AAGITLHEAIKACDTLQQEGIAVRLIDVYSVKPIDGQTLHAAAQATGGRIVIVEDHWP
                     QGGLGEAVLEAFTDNPETQGAPLNLRVRHLAVKDMPGSGTPQELLDAAGISASHIADA
                     VRALAK"
     gene            complement(4819..5469)
                     /locus_tag="VF276_09680"
     CDS             complement(4819..5469)
                     /locus_tag="VF276_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6607170.1"
                     /translation="MSTIRVLVVEDHTLVRAGMRALLRNMPGVEVVAEAGDGHTALRL
                     IEQYRPDVVLMDIALPGLNGLDTLVEAKQYPGTHVVILSMHANDEYVRRALQAGAVGY
                     LLKGSDPPELELAVRAGARGDFYLSPAVSRHVVASYVRHVDEERNSAEQLTPRQRQIL
                     QLIAEGRTTREIATILTIGTKTVETHRAHLMERLDIHDAAGLVRYAIRAGMIMPVE"
     gene            complement(5520..6890)
                     /locus_tag="VF276_09685"
     CDS             complement(5520..6890)
                     /locus_tag="VF276_09685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX6607171.1"
                     /translation="MDTQLALDPHAAQGTLIAPPSVFFETIKEGVVTLTAEHTIAYCN
                     TPLTQMLKSDRDSILGSSIFQFVASAGRSALATLLESAGQEATITLVARDGAAVPVTV
                     ACDRMYLADGAVCCLIVQDLTRQVPDDLYRLISASGRDLILLCDPAGRYLFAGASLQR
                     MLGYDPIDVIGSNLLDGFLPEDQAAAREQWGRLGALGVATLTGRYRHADGSWRILAIS
                     ATAVLHSGAPYVFALGQDVTEQQRVASALRRSLEAQENERRHLARELHDEIGQVLTAV
                     KIHLQAIQQAPRRTTRAALLAEGQDIVNQALEQVRDLSLGLRPSLLDDFGLVTAMQWY
                     ANRVAEHTGLIVQFVADPLESRLPPILETTCFRVTQEALTNVLRHAAARQAGVELRRQ
                     GDTLRLTIQDDGIGFDVRNSLEQAVHGASLGLLGMHERVLLAGGQITIQSAPAQGTQI
                     TVCFPL"
BASE COUNT         1139 a         2256 c         2400 g         1269 t
ORIGIN      
        1 gtccgcggcc acgggcagcc ggccggtgga gctgatcagc gcctcggtgc cggtgcgcag
       61 atcatgcgag tagagaccga tgtggccggt ggtggcgtcc tggtagtaga gcacaccatt
      121 gtccacggcc accccgccga actcgtgacg gacggcatcc gtgatgatcg tcgactcggt
      181 gtgggtttgc aggttgtagc tgtggatcga ttgattaccc tgagccgtgc tctccagcca
      241 gaccagtgtc atgccgtcgg tggcgatcag gggtatgtcg gtcacgatgt tcttgataag
      301 ggagatttgc cgctgctcca cgtcgcccgc gtacagggcc agatgcggcg ggccgggcgg
      361 ttgcggggtc gccgtgttga gcgggttgcc ctcgtcgcta aggtagaaca catactggcc
      421 gcccgccacg ggctcaccga tgacacggct ggcggcgagg cggatatccg gccccggcgt
      481 gggcgagccg gcggggttgg tcggcaccgg cgggggatag atcgccgtgg gcgacggcac
      541 cgccggggag ggcgtcagtt ggccgggcgg cacggcggtg gcgaaggcgt gcgccagcag
      601 gtcggcggct atcgtcccgc cgcccctacc atccggcact ccccagacca gccagtcgcc
      661 ggcgatgacc gggaattcct gatcgccggg caagattttc agggcggtga tctggcgatc
      721 acccagcttc atgccccaga tatcgaggcc gttgcccagt ccgccgggct ggttccagac
      781 cagcgtgtcg ccggccagca ccaggttaat ggcgaaatag gtgatggtgg cctggtcgcc
      841 ggtgtccagg ttacggtatt ccagttcgaa atcggcccag aagacgcgat gatcgccgat
      901 aacatagctg gcccctcgcc catccgagag ctgggtgaca gtctgggtgg tgccggtgtg
      961 gcggttgtaa gcgcgcagag tgtcgggcgg gccggtgccg ggcgcgccct cgctgtattc
     1021 gctccagatg atgtagtcgt cgctgagggc gggcgggccg aaggcgagcg ggctgggcag
     1081 cgtcgccacg acagtaatat tggggctgcc gatgtcgcgc gtcagcaggc ggctgttccc
     1141 cgtgcccgtg tcgcgctcga tccagaccac cgtctggccg tagatcgccg gctcgatgtt
     1201 gcgcagggcg cccgccgcgg ccaccgtgaa gacgccgccc gtcagcaggt tcttacccgc
     1261 gatcgccacc gggcatgtgc cgcaaacttg cgggtgctcc tcccagacga cgtttggccc
     1321 cgataccgcc acattgatct ggtcgccggg cgcgtcggtg acggtgagca gtttgcccat
     1381 ttccaggtcc agcgccatga tgttgcccgt cccggcgctg tcgggggtcg ccagactgtc
     1441 ggtccacacc aggtagccgc cggtgccctt gggacgcgac tgggcgtgcc ccggcgcggg
     1501 cggcgggatg accggcggcg tatagcgcgc gtggtagcgg aaggtgccca actggctcag
     1561 caagacctcg tagggcgagc cggcgttctc cgggtgcaac tcgaacacgg cgcgctggaa
     1621 atactgcacc gtgtagacct tgccgtcgag cgggctgcgc tcggtgaact cgtcgctgat
     1681 gggatagccc tgctgatcca gcccgccgtg gctttcccag taggcgcgga acttgccgcc
     1741 gacgtagtgc ttcgtctccg ggaaaaaccg cgcgttgtcg gtgctggcgt gctggttggg
     1801 tgcgcccgcc gtgccgtagc gcttctggta ctcaaaggta cccaccaggc tgagcaatac
     1861 atcgtagggc gcggcgtttt cggggtgcca ctcaaagacg gcgcgctcga aatactgcac
     1921 catatagggc tggccgttgg tgtcggagac ctcctgcatc ggctcgctga gcgggtagcc
     1981 ttgctgggcc aggccgccgt gctggtccca gtaggcgagg aaacgcccgc tgacggtgtg
     2041 cccggtcggc gggaaatagc ggctgtcgtt ctgtgcggcg gcgcgctcca tggcgaagcc
     2101 ggcaaccgcc aggaggaatg cgcccacgat caataccatg agtcccttgg atcgtaacct
     2161 gttcatcgcc ccatcctccg tgctgccctg cgtggacaaa gcgggttatc gtgcgtgctc
     2221 tcaccatgat caacgaggcc gctgatcgcc ggttgcaatg ggtaggctcc gttggaatca
     2281 aggcgcagag gtagtagggc tgcgcgttcc attgcgcggc ctgctgcaat cgccgggccg
     2341 cgcctcctga caaagatacc gcaaaaacgg cgcatctcgg ataggagggg aatcaggcag
     2401 ttgcaccaca tgccggggtg ctgtgcatct gactaattat cagattgtaa gaattgcata
     2461 tgcttgtcat tcttacatag actcgcaagg agcacccgca ctatactgtg gagtatagaa
     2521 tccggttgca cggatcgcgg catgactgtg cagccggatt cctttcgtta gccccgccgc
     2581 ctttccccgc ccctgatggt ccggctgccc cgtgtcgtgt tggcgcacct tgagcgcggc
     2641 gcggggacag tagaccgggc cggtcacagg cacaaagaga gccgagtccg gcaacgcacc
     2701 ggactcggct catgttatat cgtcgctgcc gtcagtgctt acttggccag ggcgcgcacc
     2761 gcgtcggcga tatggctcgc gctgatgccg gcggcgtcca gcagttcttg cggcgtgccc
     2821 gaaccgggca tatccttcac cgccaggtgg cgcacgcgca ggttcagggg cgcgccctga
     2881 gtttctggat tgtcggtaaa ggcttccagc actgcttccc ctaacccgcc ttgcggccag
     2941 tggtcctcga cgatgactat acgcccaccg gtggcctggg ccgccgcgtg cagcgtctgg
     3001 ccgtcaatcg gcttgaccga gtagacgtcg atcaggcgca cggcgattcc ttcctgttgc
     3061 agggtgtcgc aggctttgat cgcctcgtgc agcgtgatac ccgcggccac caccgtcacc
     3121 tggtccttat cggactggcg caccaccttg ctgccgccga tggtgaactg ctcgtcgggc
     3181 gggtagagca cggcggtctt ctcgcgcgtg ctgcgcagga aactgatgcc gggccggtcg
     3241 gccatggcgc ccacgagttg cgccgtctgg ttcggatcgc ttgggtagag cacggtgctg
     3301 ccgtggacag cgcgcatcat cgccaggtct tccagcgcca tctgcgacgg cccgtcctcg
     3361 ccgatggaga cgcccgcgtg tgagccgcac agccgcaggt tggcgcggga aacggccgcc
     3421 atgcggatga agtcgtaggc gcggctcagg aaggcggcga aggtcgaggc gaaggccacc
     3481 cggtggcgca cgctcatgcc caccgcggcg gcgaccattt gctgctcggc gatatacatt
     3541 tcaaagaagc gatccggcgc gaccttcttg aattcctcgg cgtgggtcga gttgctgacc
     3601 tcggcgtcga gcgcgacgac atcgggccgg gcggccagcg cggccagcgc gtcgccgtac
     3661 gctttgcgcg tggcgacctt gtcgcccacc ttgtaggtcg gcaactcgac cttcgcgttg
     3721 tcgccctggg gcggcggggc gccctggcgc tccggcttct gcacctggac ctggatgttg
     3781 gtctcgccgc ccagttcctg gatagcctgt ttggcctggt cggcgtcgag cgccttgccg
     3841 tgccagcctt ctttgttctc caggaaggag acgccgcgcc ccttcattgt cttcgccacg
     3901 atcaacgtcg gctggttttg ctgctgctcg gcttcggcgt aggcggcgtc gatggcggcc
     3961 aggtcgtgac cgtcgatctc gacggcatgc cagccgaagg cgcgcgcccg ctcggcatag
     4021 gcgccgctgt tccagcccag ctcggtctcg ccgcgctggc ccaggcggtt catgtcgagg
     4081 atgccgatca ggttgttgag gccgctgtgc ccggcgtggt cgaatgcctc ccagatcgag
     4141 ccttcggcca tctcgctgtc gcccagcaac acccagacgt ggtaggggat cttatcgagt
     4201 cgcttgcccg ccagcgccac gccgacgccg atgggcaggc cctggccgag cgagccggtc
     4261 gccacatcca cccagggaat gatcggcgtg gggtggcctt gcagccggct gccgaacttg
     4321 cggaaggtca gcatctcgtc gaggctgacg gcgcccgccg cccggtacat cgcgtagagc
     4381 aggggcgagg cgtggccctt cgagaaaatc aggtggtcgt tggccgagtt ttgcgggtcc
     4441 tggaaatcat agcgtagata cttgctcatg agcactgcca tcaggtcggc ggcggacatc
     4501 gaggacgtgg ggtggcccga ccccgcggcg gtgctggagc ggatgctgtc cacgcgcagt
     4561 tgttgggcca gcgcgcgcca gttctctgtc tgggggccgg tggcggcccg ccccgcggtt
     4621 tgtgtggtca tgaaatacct cctccggccg cagtaaagcg gcgaccatgg atacgataca
     4681 aggccgttcc ccctggtcgc tgcaaatgac gtgccatgcc tggacaggcg tcacgcagca
     4741 cgtgagcgcg cgccgcgggc cggcctggcg gtccggcgca aagcagacac gggaggagta
     4801 tttccgggcg gcccgccgct attcgaccgg catgatcatg ccggcccgga tcgcgtaacg
     4861 cacgaggccg gcggcatcgt gaatatccaa acgctccatc aaatgcgcgc ggtgggtttc
     4921 gacggttttt gtgccgatag tcaggatcgt ggcgatctcg cgtgtggtac ggccctcggc
     4981 aatcaactgc aggatctggc gctggcgggg cgtcaactgt tcggcagagt tccgctcctc
     5041 gtcgacgtgc cgcacatagc tggcgaccac gtgcctggat accgccggac tcagatagaa
     5101 atcgccacga gcgccggccc gcacggccag ttcgagttcg ggggggtccg acccttttag
     5161 caggtagccg accgcgccgg cctgcagcgc ccggcgcaca tattcgtcgt tggcatgcat
     5221 ggacaggata acgacatgcg tgccggggta ttgcttggcc tccaccaggg tatcgaggcc
     5281 gttcaagccg ggcagcgcga tatccatcag gacgacatcc gggcgatact gttcgatcag
     5341 gcgcaaggcc gtgtgcccgt cccccgcctc cgccaccacc tccacgccgg gcatgttgcg
     5401 caagagcgcg cgcatcccgg cccgcaccag ggtatggtcc tcgaccacca gcacgcggat
     5461 ggtactcacc ggaaggccct cctctcacct ccacgcgcgg ccccggtcgc cggtggagat
     5521 cacaaaggga agcagacggt gatctgagtg ccctgggccg gggccgactg gatggtgatc
     5581 tggcctccgg ctaagagcac ccgttcgtgc atgcccagca aacccagact ggctccatgc
     5641 accgcttgtt ccaggctatt gcgcacgtca aacccgatcc catcgtcttg aatcgtcagc
     5701 cgcagcgtgt cgccctgacg ccgcaactcc acgccggcct gccgggctgc ggcatgccgg
     5761 agcacattgg tcaacgcttc ctgggtgacg cggaagcagg tggtttccag gatgggcggc
     5821 aggcgcgact ccaggggatc ggccacgaac tgcacgatga gccccgtatg ctcggccacg
     5881 cggttcgcgt accactgcat ggccgtgacc aggccgaaat cgtcgagcag ggatgggcgc
     5941 aagcccaacg acaggtcgcg cacctgttcc agcgcctgat tcacgatgtc ctggccctcg
     6001 gccagcaggg cggcccgtgt ggtgcggcgc ggcgcctgct ggatcgcctg caggtggatt
     6061 ttcacggccg tcagcacctg cccgatctcg tcgtggagtt cccgcgccag atggcggcgc
     6121 tcattttcct gggcctccag gctgcgccgc agcgccgacg cgacacgctg ctgctcggtg
     6181 acatcctgac ccagggcgaa cacatagggc gcacccgagt gcagcaccgc ggtggccgag
     6241 atcgccagga tgcgccagga gccgtccgca tgccggtacc ggcctgtcag ggtcgccacg
     6301 cccagcgcgc cgagccggcc ccattgttcg cgggccgccg cctgatcttc gggcaggaac
     6361 ccatccaata gattcgaccc gatgacatcg atggggtcgt agccaagcat gcgctgtaac
     6421 gaggccccgg caaacaaata gcgccctgcc ggatcgcata gcaggattaa gtcgcgtccg
     6481 gacgcgctga tgagccggta gaggtcgtcg ggtacttgcc gggtcagatc ctgtacgatc
     6541 aagcagcaca ccgcgccgtc cgccaggtac atgcggtcac aggcgacggt aaccggcacc
     6601 gcggcgccgt ccctggccac cagggtgatg gtggcttctt gcccggcgga ttccaacaga
     6661 gtcgccagtg cggaccggcc ggcggaggcg acgaattgga aaatagagga acccagtatg
     6721 ctatcgcgat cgctcttcag catctgcgtt aggggcgtat tgcaatacgc gatggtatgt
     6781 tcggctgtca gcgtcaccac gccttccttg atggtctcga agaacacgga aggcggcgcg
     6841 atgagagtgc cctgcgcggc atgcgggtct agggccaact gagtgtccac agcatcctcc
     6901 agcaccaggt aaggaacatg tgacgcaaac gaccgcgcct tgagagtccc gtcggtgtta
     6961 gtgcagcggt gacaagccct tgggtgcggt tgggtgaccg gcgcggtggt agctaacaac
     7021 aagcagcagt agggttagct cagatcacaa aagcgtgggt ggca
//
LOCUS       DASUYC010000595         2722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_577717,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000595 DASUYC010000000
VERSION     DASUYC010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577717"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="VF276_09690"
     CDS             <1..942
                     /locus_tag="VF276_09690"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX6607172.1"
                     /translation="PVNIATQQLHDAGITVVFAAGNSGANTNTLNTYSVAPWVIGVAA
                     GEKDGHTVAFFSSRGIPGDSFYHPTLTAPGYLIASDRTATGAFVNGGSTATDAAFIAP
                     QYLPYYTVASGTSMAAPHVAGTIALIVQANPSLTPDVIKRVLINTARPIPGAQEYAAG
                     AGYLDALAAVQTARSIRNIHTYRDPRTGREEQVYDLVGTWTGTVGLSAPGLPSSDSRQ
                     IVVEPGTESLEVAIDWNLAGTGQTGVVSDLNLYLYDPSGQLAASSEVVQTVYNYANET
                     VHVDLPVAGTWTATVTGFLNAPQDYSGTSSAVVLVNP"
     gene            1114..2235
                     /gene="cax"
                     /locus_tag="VF276_09695"
     CDS             1114..2235
                     /gene="cax"
                     /locus_tag="VF276_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014405743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HEX6607173.1"
                     /translation="MNAFAQALAYVGLDIKGPESAKLFERVIYGLLIFLPIAVLVKLL
                     GLPELALFITSALAIVPLAKILGTATEELASRVGSGLGGLLNATFGNATELIIAIVAL
                     NAGLTEVVKASITGSIIGNVLFVLGLAMFLGGLGREKQLFNRTGASANSSQFALAFVG
                     LAVPAMYVLATNNDNFLTEEEISVGVAIILLLAYIAQLVFFLRTHAFLYTDSEEQTMH
                     GARWTIPHALIVLAVATLLIAGLSELLVEGVEYLTSTLGWSEAFVGVVLVAIIGNAAE
                     HMTAVTVAMRDRMELALSIATGSALQIALFVAPVLVFVGLLLGKPMNLVFTLFELVAV
                     GVALLITILVSQDGESNWLEGMQLLAAYAILAVAFFFVP"
     gene            complement(2244..2543)
                     /locus_tag="VF276_09700"
     CDS             complement(2244..2543)
                     /locus_tag="VF276_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607174.1"
                     /translation="MGLVVGFLVIYLVILVLLLVVGVGVGFVLHWLIPGVDMGLAVLI
                     GVLTSGMAIHFYGRLTGLIEEAEPLDEEPPPGRPVSVRMYPVEQFAPRRSRKRKP"
BASE COUNT          460 a          917 c          870 g          475 t
ORIGIN      
        1 ccggtgaaca tcgcgacgca gcaactccat gacgccggga tcacggtagt cttcgccgcg
       61 ggcaactccg gcgcgaacac aaacacgctc aacacctaca gcgtggcgcc ctgggtcatc
      121 ggcgtggcgg cgggcgagaa ggacgggcac accgtggcct tcttctcctc gcgcggcatc
      181 cccggcgact cgttctacca ccccacgctg accgcgcccg gctacctgat cgcctcggac
      241 cgcacggcca cgggcgcctt cgtcaacggc ggctccacgg cgaccgacgc cgcgtttatc
      301 gcgccgcaat acctgccgta ctacaccgtc gccagcggca ccagcatggc ggcgccccat
      361 gtagcgggca ccatcgcgct catcgtccag gccaacccca gcctcacgcc cgacgtgatc
      421 aagcgtgtgc tgatcaacac cgcccgcccg atccccggcg cgcaggaata cgcggcgggc
      481 gccgggtatc tcgatgccct ggccgccgtg cagacggcgc gatccatccg caacatccac
      541 acctaccgcg acccgcgcac cgggcgcgag gaacaggtct atgacctggt cggcacctgg
      601 accggcacgg tcggcctgag tgcgccgggg ctgcccagca gcgacagccg ccagatcgtg
      661 gtcgagcccg gcaccgagtc gctcgaagtg gcgatcgact ggaacctcgc gggcaccggc
      721 cagaccgggg tcgtcagcga tctcaacctc tacctctatg accccagcgg tcaactggcc
      781 gccagttccg aagtggtgca gacggtctac aactacgcca acgaaacggt gcatgtggac
      841 ctgccggtcg cgggcacctg gacggccacc gtcaccggct tcctcaacgc gccacaagac
      901 tacagcggca caagtagtgc cgtcgtgctc gtgaacccgt agcccggccc ggatcgctgt
      961 tcggatgccc ggctccggcc gggcatctgc atgtctgcca ctggattcgt aaatacgaat
     1021 agtgtatcat gggacgcacg gctggaggcg gcgcgcccgg caccggggcg gcggccggtt
     1081 ctctccggct catcttcagc tcaggagttg cgtatgaatg catttgccca ggccctggcc
     1141 tatgtcggcc tggatatcaa aggcccggaa agcgccaaac tgttcgagcg cgtgatctac
     1201 gggttattga tctttctgcc gatagccgtg ctggtcaagc tgctgggcct gccggaactg
     1261 gcactgttca tcaccagcgc cctcgccatc gtgccgctgg cgaagatcct gggcacggcc
     1321 accgaggaac tggcctcgcg ggtcggctcc gggctcggcg gtctgctcaa cgccaccttc
     1381 ggcaatgcga ccgagctaat catcgccatc gtcgcgctga acgccgggct gaccgaggtg
     1441 gtcaaggcgt cgatcaccgg gtcgatcatc ggcaatgtgc tgttcgtgtt ggggctggcg
     1501 atgtttttag gcggcctggg ccgcgaaaag cagttgttta accgcacggg cgccagcgcc
     1561 aactcctcgc agttcgccct ggcctttgtc gggctggcgg tgcccgccat gtatgtcctg
     1621 gcgacgaata acgacaactt cctgaccgag gaagaaatct cggtcggcgt ggcgattatc
     1681 ttgctgctgg cttatatcgc ccaactggtg ttcttcctgc gcacccatgc cttcctctac
     1741 accgacagcg aggagcagac catgcacggc gcgcgctgga cgatcccgca cgcgctgatc
     1801 gtgctcgcgg ttgccaccct gctcatcgcc gggttgtccg aactgctggt cgagggcgtg
     1861 gagtatctga ccagcacgct gggctggagc gaggcgtttg tcggcgtggt gctggtcgcg
     1921 atcatcggca acgcggcaga gcacatgacg gcggtgacgg tcgccatgcg cgaccgcatg
     1981 gaactggccc tgtcgatcgc caccggcagc gccctgcaga tcgccctgtt cgtggccccg
     2041 gtgctggtgt tcgtgggcct gctgctgggc aagccgatga acctggtctt cacgctcttc
     2101 gagctggtgg ccgtcggcgt ggcgttgctg atcacgatcc tggtctcgca ggacggcgag
     2161 agcaactggc tggagggtat gcaactgctg gccgcctacg ccatcctggc ggtggccttc
     2221 ttcttcgtgc cctgaccggc ggattagggc ttgcgcttgc ggctacggcg cggggcgaat
     2281 tgctcgacgg gatacatgcg cacgctgacg ggccggccgg gtggcggttc ttcgtcgagc
     2341 ggttccgctt cctcgatcag ccccgtcagc cgtccgtaaa aatggattgc cataccggac
     2401 gtgaggacgc cgattaggac cgccagcccc atgtcgacgc cggggatcag ccagtgaagc
     2461 acgaagccga cgcccacgcc gacgaccagg aggagcacca ggatcaccag atagataacc
     2521 aggaagccga ctaccaggcc cataccaccc cgcctttcca cgctggcgga ctgttatccg
     2581 cagacgtgtg tgcaatcgta tcaattatag tgccgtcgtg ggccggaacg cggatcagcc
     2641 ttccgaacga gggccggccc gtcgcggcgc ggttgcgtgg ggccgattgg atagcgtata
     2701 atggacaatg caccgtgcgc gg
//
LOCUS       DASUYC010000596         4257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1885671, whole genome shotgun sequence.
ACCESSION   DASUYC010000596 DASUYC010000000
VERSION     DASUYC010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1885671"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VF276_09705"
     CDS             <1..544
                     /locus_tag="VF276_09705"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEX6607175.1"
                     /translation="VNGSDFFYRPVQYLFCRGVISGYADGTFRPNNNTTRGQFAKMLV
                     LGRGWPVVTPAAPTFRDVPDTYPFYGYIETATQHGAISGYDCGPGCREFRPGNNVTRG
                     QISKLIAVALGWTLTTPATPTFRDVDATNPFYAAIETVYAHGVISGYDCGPGCLEFRS
                     ANNATRAQLSKMLWVAVTHP"
     gene            complement(639..1799)
                     /locus_tag="VF276_09710"
     CDS             complement(639..1799)
                     /locus_tag="VF276_09710"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEX6607176.1"
                     /translation="MTWGMLLVAGVVLAASLILTPAMRRLALRRGWYDLPADRRVHTT
                     PTPRIGGVAMYLAFMIGLALTLVPGVTPERLEPPFGPPELWRIGLLALGATIITAVMF
                     VDDIRGIKPIPKLLWQLFVAALVMLPDPRYLDLAPGAVNGVPNGWFTGVIVSFLQNPF
                     GSHDSLQIQLFQFAPLAIAFTFFWIVGMMNAVNWMDGLDGLAGGVSAIACLVLFGVSL
                     LQNPPQTTIAFLPLILAAAIFGFLRYNFHPAQIFMGDSGSMFLGFALAVISIIGGAKV
                     ATALLVMGVPILDVAYVILFRVLRGRSPLQADRGHLHHRLYDLGMGQSQIALLFYFVC
                     GGFGALALLPPGNGLIKLGALVVMAVLLIALILFITRRQFDRAQRTDPDLQS"
     gene            complement(1803..2429)
                     /gene="upp"
                     /locus_tag="VF276_09715"
     CDS             complement(1803..2429)
                     /gene="upp"
                     /locus_tag="VF276_09715"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEX6607177.1"
                     /translation="MSQVYVSNHPLILHKLALLRSVTTEPKKFREVVAEITQLLTYEA
                     TQDLSTVEEEVVTPMATTTCRVVDERVGLVPVLRAGLGMVEAVLQLLPYAQVWHIGLF
                     RDEKTLKPIEYYNRLPDHSSVDTCLLLDPMLATGGSASATVDILKKWGAGRIKYVGLI
                     AAPEGIAHLSAQHPDVPIHVAAVDSHLNEIGYIVPGLGDAGDRQFGTG"
     gene            complement(2442..3731)
                     /gene="glyA"
                     /locus_tag="VF276_09720"
     CDS             complement(2442..3731)
                     /gene="glyA"
                     /locus_tag="VF276_09720"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX6607178.1"
                     /translation="MQTLTKLTGEGQAAGQDPPLAQADPAIYEAIQQEQERQRMKIEL
                     IASENYVSKAVLEAQGSVLTNKYAEGYPGKRYYGGCEYVDVVERLAIERLLELFGAEH
                     ANVQPHSGAQANMAAYMAVLQPGDTILGMSLAHGGHLTHGYKINFSGQLYHATSYGVA
                     EGSERLDYDALAAQVLEEKPKMLVAGASAYPRIFDFPRLREIADSVGALLFVDMAHIA
                     GLVATGYHPSPVPYADIVTSTTHKTLRGPRGGLILCKAQYAKAVDKTVFPGVQGGPLM
                     HVIAAKAVAFGEALRPEFKTYAGQIVANAQALAGHLEAGGLRLVSGGTDNHLMLVDVT
                     PLGLTGKQAEHALDQVGITVNKNAIPFDKQPPAYASGIRIGTPAVTTRGFGLPEMATI
                     GRLIIDVLKHHEDAAVLARVSDEVRVLCSRFPVPGIE"
     gene            4027..>4257
                     /locus_tag="VF276_09725"
     CDS             4027..>4257
                     /locus_tag="VF276_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607179.1"
                     /translation="MPAFHIVQLERFLRGNQGRITQLIDEVYERTGGHYTALPPEQRR
                     RQAAGDTEEFIIDLLRGSVDRDSTRQGLAAIAD"
BASE COUNT          751 a         1432 c         1395 g          679 t
ORIGIN      
        1 cgtgaatggg agcgacttct tctaccggcc ggtgcaatac ctgttctgcc gcggggtgat
       61 cagcggctac gccgacggca ccttccgccc caacaacaac accacgcgcg gccagttcgc
      121 caagatgctg gtgctgggcc gcggctggcc cgtcgtcacg cccgccgccc ccaccttccg
      181 tgacgtgccc gatacctatc cgttctacgg ctacatcgaa accgcgacgc agcacggcgc
      241 gatttcgggc tacgattgcg gccccggctg ccgcgagttc cggcccggca acaacgtcac
      301 tcgcggccag atcagcaagc tgatcgccgt cgcgctgggc tggacgctca ccacgcccgc
      361 cacgcccacc ttccgcgatg tggacgcgac gaacccgttc tacgcggcca tcgagaccgt
      421 ctacgcgcac ggcgtgatct ccgggtacga ttgcggtccc ggctgcctgg agttccgctc
      481 ggccaacaac gccacgcgcg cccagttgag caaaatgctc tgggtcgcgg tcacgcatcc
      541 ctaaagggta cagggctatc tcggtggggc gccgggcgcg ataaatcgcg ccgctacgta
      601 gcgttatccg tagcggcgca gttcattgcg cccggtgctt agctttgtag atcggggtcg
      661 gtgcgctggg cgcggtcaaa ctggcggcgg gtgatgaaga ggataagggc gatcagcagg
      721 acggccatga cgaccagcgc gccgagctta atcaggccgt tgcccggcgg cagcaaggcc
      781 agcgcgccaa agcccccgca gacgaagtag aacagcagcg cgatctgcga ctggcccatg
      841 cccaggtcgt acagccggtg gtgcagatgc ccgcggtcgg cttgcagggg cgagcgcccg
      901 cgcaacaccc ggaagaggat cacataggcc acatcgagga tcggcacgcc catgaccagc
      961 agcgccgtcg ccaccttggc cccgccgatg atcgagatca ccgccagcgc gaagccgagg
     1021 aacatcgagc cgctgtcgcc catgaagatc tgggccgggt ggaagttgta gcgcaggaag
     1081 ccgaagatcg ccgcggccag aatcagcggc aggaaggcga tggtcgtttg cggcgggttc
     1141 tggagcaggc tcacgccaaa gaggaccagg caggcaatcg cggagacgcc ccccgccagc
     1201 ccgtcgaggc catccatcca gttgacggcg ttcatcatgc cgacgatcca gaaaaaggtg
     1261 aaggcgatgg ctaacggcgc aaactggaaa agctggatct gcaggctgtc gtgcgagccg
     1321 aaggggttct gcaaaaagct gacgatgacg ccggtaaacc agccgttggg cacgccgttg
     1381 accgcgccgg gtgcgaggtc caggtagcgc ggatcgggta gcatgaccag ggcggccacg
     1441 aacagttgcc agagcagctt ggggatcggt ttgatgccgc ggatgtcgtc gacaaacatc
     1501 acggcggtga tgatcgtcgc accgagggcc agcagtccga tgcgccagag ttccggcggg
     1561 ccaaagggcg gctccagccg ttcgggcgtc acgccgggca ccagggtgag cgccaggccg
     1621 atcatgaagg ccaggtacat cgccacgccc ccgatgcgcg gggtgggcgt tgtatggacg
     1681 cgccggtcgg cggggagatc gtaccaaccc cggcgcagcg ccaggcggcg catggccggg
     1741 gtcaggatga gcgaggccgc caggaccaca cccgccacga gcagcatccc ccaggtcatg
     1801 ccctacccgg tcccaaactg acggtccccc gcatcgccca ggccggggac gatatagccg
     1861 atctcgttca ggtggctgtc caccgccgcc acatggatgg gcacatcggg gtgctgcgcg
     1921 ctcaggtgcg cgataccctc gggcgcggcg atcaggccca catacttgat gcggcccgcg
     1981 ccccacttct tgaggatgtc caccgtggcc gacgccgacc cgccggtcgc cagcatcggg
     2041 tcgagcagca ggcaggtatc gaccgagcta tggtcgggca gccggttgta gtactcgata
     2101 ggcttgagcg tcttctcgtc gcggaacagg ccgatgtgcc agacctgggc atagggcagc
     2161 agttgcagca cggcctcgac catgcccaac cccgcgcgca gcaccggcac caggcccacc
     2221 cgctcgtcga ccacgcggca ggtcgtggtc gccatcgggg tcaccacctc ttcctccacc
     2281 gtggacagat cctgcgtggc ctcataggtc agcagttgcg tgatctcggc caccacctcg
     2341 cggaatttct tcggctccgt ggtgacgctg cgcagcagcg ccagcttatg caggatcagc
     2401 ggatgattcg agacatagac ttgcgacata gaccctctcg cctactcgat gccgggcacc
     2461 gggaagcgcg agcagagcac gcgcacttcg tcgctgaccc gcgccagcac cgcggcatcc
     2521 tcatggtgct tcagcacgtc gatgatcaac cgcccgatgg tcgccatctc cggcaagccg
     2581 aagccgcggg tggtcacggc aggggtgccg atgcggatcc cgctggcgta ggccgggggc
     2641 tgcttgtcga acgggatggc gttcttgttc acagtaatgc cgacctggtc gagcgcgtgt
     2701 tcggcttgct tgccggtcag ccccagcggg gtgacatcga ccagcatcag gtggttgtcg
     2761 gtgccgccgg agaccaggcg cagtccgccc gcttccaggt gcccggccag cgcctgggcg
     2821 ttggcgacga tctgcccggc gtaggtcttg aactcgggcc gcaacgcttc gccgaaggcc
     2881 accgccttgg cggcgatcac atgcatcagc gggccgccct ggacaccggg gaagacggtc
     2941 ttgtcgaccg ctttggcata ctgggccttg cagaggatca gcccaccgcg cgggccgcgc
     3001 agcgtcttat gcgtggtgct ggtgacgatg tcggcgtagg gcaccggcga gggatggtag
     3061 cccgtagcaa ccagcccggc gatatgggcc atatccacga acagcagcgc gcccacgctg
     3121 tcggcgatct cgcgcagccg ggggaaatcg aagatgcgcg ggtaggccga ggccccggcg
     3181 accagcatct tgggcttctc ctccagcacc tgggcggcca gcgcgtcgta gtcgagccgc
     3241 tcgctgccct cggccacgcc atacgaggtg gcatgataca actggccgga gaagttgatc
     3301 ttgtagccgt gggtcaggtg gccgccgtgg gccaggctca tgcccaggat cgtgtcgccc
     3361 ggctgcaaca cggccatata ggcggccatg ttcgcctgcg ccccgctatg cggctgcacg
     3421 ttggcgtgct cagcgccgaa cagttccagc aggcgctcga tggccaggcg ctcgaccaca
     3481 tcgacgtact cgcacccgcc atagtagcgc ttgccggggt agccttcggc gtacttgttg
     3541 gtcagcaccg acccctgcgc ctccagcacg gccttgctga cgtagttctc cgaagcgatc
     3601 agctcgattt tcatgcgctg gcgttcctgc tcttgctgga tcgcctcgta gatcgccggg
     3661 tcggcttgcg ccagcggcgg atcctggccc gcggcctggc cctctccggt caatttggtc
     3721 agcgtttgca tacaactcct cctaccgcgg tcgcgcgccc cggcggggcg aagagacgaa
     3781 aaccctggaa cagggtggcg tgaatgtcgc ccccattata cgcgccactg cgggtagcgt
     3841 caacgcggcc ccgtcaggct ggcgacgaaa gatggttgct cgcgtcgaga tgtgtgctat
     3901 aataccccct gtatcgtcta cgccctgcct gctaccaggg cctgccgccg gactcgctac
     3961 cggcacacat cgactatgta ccctagccgg gaagctaccc gcccgcgcaa cacccagagg
     4021 atgcccatgc cggctttcca tatcgtccaa ctcgaacgct ttctccgcgg caaccagggc
     4081 aggattacgc aattgatcga cgaagtttac gaaaggaccg gcggccatta caccgccctg
     4141 ccgccggagc agcgccggcg ccaggccgcc ggcgataccg aagaattcat catcgacctg
     4201 ttgcgtggca gcgtggaccg cgactcgacc cgccagggtc tggccgcgat cgccgac
//
LOCUS       DASUYC010000597         2360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_267463,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000597 DASUYC010000000
VERSION     DASUYC010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267463"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /gene="galT"
                     /locus_tag="VF276_09730"
     CDS             <1..960
                     /gene="galT"
                     /locus_tag="VF276_09730"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEX6607180.1"
                     /translation="AGQMVAVPPARPPAYVPDCYLCPGNLRVSGARNPDYTGVFVFDN
                     DHPCVGPEAPQHLSPPPPPYANRPATGLARVICYSPRHDLTLAEMPVPTIAAIAGVWQ
                     QQTRDLGNRPEVRHVLCFENKGEVVGVSNPHPHGQIYATNFVFKTIATELAAAERYRR
                     ETDRLLFSDIIAAEQTDGRRILYEDAHAIAFLPYFARYAYEVYVAPKRPVAHIYALRD
                     EEIEALARALKHVTVAFDNLWEMPFPYVMPLHQAPADDGDYSMFHFFIAFHPPLRRPN
                     VLKYLAGPEIGGGNFLSDTTPEAKAAELRAVSPIHYRERGTPP"
     gene            957..2000
                     /locus_tag="VF276_09735"
     CDS             957..2000
                     /locus_tag="VF276_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HEX6607181.1"
                     /translation="MIRRAADLLHQIRRIHEAIRDAVVAACEATPLETLAGVASDAPG
                     DTIYTIDRVSEDVLLEHFHDLAQEWSCILIAEGLGASGTAVLPAGSDPAQAELRIIVD
                     PIDGTRGLMYQKRSAWILTGVAPNRGPATSLRDIELAVQTEIPLVKQHLSDCLWAGGG
                     TEAGGERYNRLTGERRALAPHPSQATTYAHGYGQIVRFFPGTRAELAAIDDAVAAAAL
                     GPPQPGKAGAFEDQYASTGGQLYELMMGHDRWIADIRPLLAPLLRARGQALGLCCHPY
                     DLCTELIAREAGVLVTDAQGRPLDAPLDVAPDVAWIGYANAAIRAQVAPVLTRLLLER
                     GWLPAEEPGDVGV"
     gene            1987..>2360
                     /locus_tag="VF276_09740"
     CDS             1987..>2360
                     /locus_tag="VF276_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HEX6607182.1"
                     /translation="MWEFEQAPLAGPPDLDRFLAVLNAHPGFFAPDRPLYLARAPGRL
                     DLMGGIADYSGALVLQLPLALATRVAAQLTGEPLLVICNPARGADSEPEIALPLAELT
                     PATEPLSYPAAHRLLTADPARS"
BASE COUNT          350 a          893 c          754 g          363 t
ORIGIN      
        1 gcgggccaga tggtcgccgt gccccccgcg cggcccccgg cctacgtgcc cgattgctat
       61 ctctgccccg gcaatctgcg tgtgagcggg gcgcgcaacc cggactacac cggcgtcttt
      121 gtcttcgata acgatcatcc ctgcgtgggg ccggaggcgc cgcagcacct ctcccctccc
      181 ccaccgccct atgccaaccg cccggccacc gggctggccc gcgtgatctg ctacagcccg
      241 cgccacgatc tcaccctggc cgagatgccg gtaccgacca tcgccgcgat tgccggcgtc
      301 tggcagcagc agacccgcga tctgggcaac cggcccgagg tgcgccatgt gctctgcttc
      361 gagaacaagg gcgaagtcgt cggcgtctcg aatccgcatc cccacggcca gatctacgcc
      421 accaactttg tcttcaagac catcgccacc gaactggcgg cagccgagcg ctatcgccgc
      481 gagacggatc gcctcctgtt ctccgacatt atcgccgccg agcagaccga cgggcggcgc
      541 atcctctatg aagacgcgca cgccatcgcc ttcctgccct atttcgcgcg ctatgcctat
      601 gaagtctatg tcgcgcccaa acgcccggtc gcgcatattt acgccctgcg cgatgaggag
      661 atcgaagccc tggcccgcgc gctgaagcat gtcacggtgg ccttcgacaa cctgtgggag
      721 atgccgttcc cgtatgtcat gccgctgcac caggcgcccg ccgatgacgg ggactactcg
      781 atgttccatt tcttcatcgc cttccacccg ccgttgcgcc ggcccaacgt cttgaagtat
      841 ctggccgggc cggagatcgg cggcggcaat ttcctgagcg ataccacgcc ggaggcgaag
      901 gcggcggaac tccgcgccgt ctcgcccatc cactaccgcg agcgagggac gccgccatga
      961 tccgccgcgc cgccgacctg ttgcaccaga tccgccgcat ccacgaagcc atccgcgacg
     1021 ccgtcgtggc cgcctgcgag gccacgccgc tcgaaacgct ggccggcgtg gcctccgacg
     1081 cgccgggcga cacgatctac accatcgacc gggtctccga agatgtgttg ctggagcatt
     1141 tccacgatct cgcccaggaa tggtcctgca tcctgatcgc cgaagggctg ggcgcgagcg
     1201 ggacggccgt gttgcccgcg ggcagcgacc cggcgcaagc cgagctccgc atcatcgtcg
     1261 atcccatcga cggcacgcgc gggctgatgt accagaagcg ctcagcctgg atcttaaccg
     1321 gggtcgcgcc caatcgtggc ccggcgacca gcctgcgcga catcgaactg gccgtgcaga
     1381 cggagatccc gctggtcaag cagcacctga gcgattgcct ctgggccggg ggcgggacgg
     1441 aggccggcgg cgagcgctac aaccggctca ccggggagcg gcgggcgctg gcgccccatc
     1501 cctcgcaagc cacgacttac gcccacggct acggccagat cgtgcgcttc ttccccggca
     1561 cccgcgccga actcgccgcc atcgacgacg ccgtggccgc cgcggcgctg gggccgccgc
     1621 agccgggcaa ggccggcgcc tttgaagacc agtatgcctc caccggcggc caactttatg
     1681 agttgatgat gggccacgac cgctggatcg ccgacatccg cccgctgctg gcgccgctgc
     1741 tgcgggcgcg cggccaggcc ctcggccttt gctgccatcc ctacgacctc tgcaccgaac
     1801 tgatcgcgcg cgaagcgggc gtgctcgtca ccgatgcgca gggccggccc ctggatgccc
     1861 cgctcgatgt ggcgccggat gtcgcctgga tcggctacgc gaacgcggcg atccgggccc
     1921 aggtggcccc tgtgctcacg cgcctgctgc tggagcgcgg ctggctgccc gctgaggaac
     1981 cgggcgatgt gggagtttga gcaagcgccg ctcgccgggc cgccggacct ggatcgcttc
     2041 ctggcggtgt tgaacgccca ccccggcttc ttcgcgccgg atcgcccgct ctatctggcc
     2101 cgtgcgccgg gccggctcga cctgatgggc ggaatcgccg attactcggg cgcgctggtg
     2161 ctgcaattgc ccctggccct ggcgacccgc gtggccgcac aactgaccgg cgagccgctg
     2221 ctggtgatct gcaacccggc ccgtggcgcc gacagcgagc cggagatcgc gctgcccctc
     2281 gccgaactca cgcccgccac cgagccgtta agctacccgg cggcgcaccg gctgctgacc
     2341 gccgacccgg cgcgtagctg
//
LOCUS       DASUYC010000598         2323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1307625, whole genome shotgun sequence.
ACCESSION   DASUYC010000598 DASUYC010000000
VERSION     DASUYC010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307625"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..1145)
                     /locus_tag="VF276_09745"
     CDS             complement(69..1145)
                     /locus_tag="VF276_09745"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEX6607183.1"
                     /translation="MTEEDLRQQRLHNLGLSTAPYATPEAVVAALGAVQAQDYPAAAW
                     ALGLRMGAATAATIDAALAAGAILRTHVLRPTWHFVAPADIRWMLALTGPRVQAAIAG
                     PSRRLGLDAAMIAAANTALADALQGSHALTRAELTAVLARAGIAATEPLRNAHLLMHA
                     ELEGLICSGPRQGKQMTYALLDERVPPTRPLTRDEALAALTRRYFTSHGPATIPDFVW
                     WSGLTGADARAGLALVGDALTSEEIDGRTYWFAPGAPPTPMPTPTAHLLPNYDEYIVA
                     YTDRRAVHDAAAHDLDARGNILFSHTIVLDGRVVGTWKRTLRRAAVVLETTLLAPLAP
                     AATAAVAAATARYGAFLGLAVEPA"
     gene            1813..>2323
                     /locus_tag="VF276_09750"
     CDS             1813..>2323
                     /locus_tag="VF276_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX6607184.1"
                     /translation="MPRIESLLAARLFLSPQLWRDRLYFISNLSGRFSLYSMPYGGGV
                     PEPLIPPDIALQNPDLTGGTPYYVYPDLGRIMVVVDKDGDEVYKPMLIPLDGGFPEPA
                     FPAITDARVFSVHGPEGSRKLYMVASSLSAAVNRTLIGDLTDGALTQIGETRWEMYPA
                     AISDDETRVV"
BASE COUNT          340 a          773 c          818 g          392 t
ORIGIN      
        1 tcccccttcc cgcgccggga agggggccgg ggggttaggc ccgtcgcgcc gggaaggggg
       61 ccggggggtt aggccggttc gaccgccaag cccaggaacg caccgtagcg cgcggtggcg
      121 gcggcgacgg cggcggtggc cgcgggggcg agcggggcga gcagagtggt ttccagcacc
      181 acggcggccc gccgcagggt gcgcttccag gtgcccacca cccgcccgtc cagcacgatg
      241 gtgtggctga agaggatgtt gccgcgcgcg tcgaggtcgt gggccgcggc gtcatgcacg
      301 gcgcggcggt cggtgtaggc cacgatgtac tcgtcgtaat tgggcagcaa gtgggccgtg
      361 ggcgtgggca tgggggtggg cggcgcaccc ggcgcgaacc agtaggtccg cccgtcgatc
      421 tcctcggacg tgagcgcgtc gcccaccagg gccaggccgg cgcgggcgtc ggccccggtc
      481 aggcccgacc accagacgaa atcggggatg gtggccgggc cgtggctggt gaagtagcgc
      541 cgggtcaggg cggccagcgc ctcgtcgcgg gtcagcgggc gggtgggggg cacgcgctcg
      601 tcgagcaggg cataggtcat ttgcttgccc tggcgcggcc cgctacagat cagcccctcc
      661 agttcggcgt gcatcagcag gtgggcgttg cgcaacggct cggtggcggc gatcccggcc
      721 cgcgccaaca cggcggtcag ttcggcgcgg gtgagcgcgt ggctgccctg caaggcgtcc
      781 gccagggccg tgttggcggc ggcgatcatc gcggcgtcga ggccgagccg gcgactcggc
      841 ccggcgatgg cggcctgcac gcgcgggccg gtcagcgcca gcatccagcg gatatcggcg
      901 ggggcgacga aatgccaggt gggccgcagc acatgggtgc gcaggatggc gcccgcggcc
      961 agcgcggcgt cgatggtcgc cgccgtcgcc gcgcccatgc gcagccccag cgcccacgcc
     1021 gccgccgggt agtcctgtgc ctgcaccgcg ccgagcgccg cgaccaccgc ctccggcgtg
     1081 gcatacggcg ccgtgctcag gcccaggttg tgcagccgct gttgtcgcag gtcctcctcg
     1141 gtcacgctgt cctccttgtg tgcaacgccg gccctctcct cgccgcgatt gtacccgatc
     1201 cgggcgcgcg ccgtccacgc cccgccccgg cgaacacgtg cgtaatttca ccgcgaagac
     1261 gcgaaggacg cgaagatgat ttatataaat gttgttcttc ttgctttttg cgccttccct
     1321 gaaagtgggg ggcgggtagc cattggggat tgtcattctg agcgcagtga agcatctcgt
     1381 ctctgcgcca cggagacgag actcttcgct gcgctcagag tgacacgccg gggagcgaga
     1441 ctcttcgctg cgctcagagt gacaagctac ctttcttccc tgaaagtggg gggcgggtag
     1501 ccattgggga ttgtcattct gagcgcagcg aagaatctcg tcttgttggg gttcacgccg
     1561 agactcttcg ctgcgctcag agtgacaaac tacccttcat acccccagtg tcccactggg
     1621 acatacccca cttcgtgatg caattccgtc tttctgcacg acgaagatca attctataca
     1681 tatcgttctt cgcgttcttc gcgccttcgc ggtgaaataa acgtcgcctc ggttggccgg
     1741 gcttgccgcg ggcggcggaa tggcgcataa tgggggcacc acgcctagtt taccctgaag
     1801 gagctatcta ctatgcctcg catcgagtcg ttactggcgg cgcgcctgtt tctctcgccg
     1861 cagttgtggc gggaccgcct gtactttatc agcaacctca gcgggcgctt cagcctctac
     1921 agcatgccct atggcggcgg cgtgcccgag ccgctgatcc cgcccgacat cgccctgcaa
     1981 aacccggacc tgaccggcgg cacgccctac tatgtctacc ccgacctggg ccggatcatg
     2041 gtcgtcgtgg acaaggacgg cgacgaggtc tacaagccca tgctcatccc gctggacggc
     2101 ggcttccccg aaccggcctt cccggccatc accgatgccc gcgtcttttc ggtccacggg
     2161 ccggagggca gccgtaagct ctacatggtc gccagttcgc tgtcggcggc ggtgaaccgc
     2221 acgctcatcg gcgacctgac ggacggcgcg ctgacgcaga tcggcgagac gcgctgggag
     2281 atgtaccccg cggccatcag cgacgatgag acccgcgtgg tgg
//
LOCUS       DASUYC010000599         2262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_594548,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000599 DASUYC010000000
VERSION     DASUYC010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594548"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="VF276_09755"
     CDS             <1..845
                     /locus_tag="VF276_09755"
                     /inference="COORDINATES: protein motif:HMM:NF028259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607185.1"
                     /translation="RMQSSGSAQRSRKRVARDAANAGWLGRAPAYLAIALKDWRVMPR
                     DLRNFAQIIGPLVMLPVIYFNLLGGGGRRSFNATAELTSLTGGRVDPTGIFIAMGVLM
                     ATSLLATQISATAISMEGKSWWLLKAAPISGIELVRGKFLAAALPFVVLSSMLMIAAL
                     IWKQFSLLGFLYGWFGVELLGCGVLAMSLGFAMRWPRLDWDNPKQMRNGWANVASLAG
                     DAVLGLLGGGLLLVPILAEIWAPAWVLPAWIIGIAGAAGVTVGLAWTALAIGLDHLPE
                     VGEA"
     gene            1197..1913
                     /locus_tag="VF276_09760"
     CDS             1197..1913
                     /locus_tag="VF276_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607186.1"
                     /translation="MDNETRHLDHEAARAAGLPADGPSAPGNPPAGNGGTPTTYLPPV
                     TNAATASEAPRPSYAPPATGPTYTPPAPGAVPAYTPPRPAGNGQARRGSGPESLIPLA
                     LIAVGILALFGGFRWVFGAAIPLALGLIFLYVSSQGPGRLGFRIPGCILTGLGAGIVL
                     DSMIGGGWSAVGLGLGFVALWVMDRAQLWWLIPGAAVGLGGLQSVWSGSDWGGNWFFP
                     LALIAFGAYLLSGRQWRARR"
BASE COUNT          295 a          790 c          801 g          376 t
ORIGIN      
        1 tgcgcatgca gagcagcggc agcgcgcaac gcagccgcaa gcgcgtggcc cgcgatgccg
       61 ccaatgccgg ctggctgggg cgggcgcccg cttacctggc gattgccctc aaggactggc
      121 gcgtgatgcc gcgcgacctg cgcaactttg cccagatcat cggcccgctg gtgatgctgc
      181 cggtgatcta tttcaacctg ctgggcgggg gcgggcgccg ctcgtttaac gccacggcgg
      241 aactaaccag cctgacgggc gggcgcgtgg accccaccgg catcttcatc gccatgggcg
      301 tgctgatggc gacctcgctg ctggcgacgc agatttcggc cacggccatc agcatggagg
      361 gcaaatcgtg gtggctgctc aaggccgccc cgatctccgg catcgagctg gtgcgcggca
      421 agttcctggc ggcggcgctg cccttcgtgg tcctcagcag catgctgatg atcgcggcgc
      481 tgatctggaa gcagttcagc ctgctcggct tcctctacgg ctggtttggg gtggagttac
      541 tgggctgcgg cgtcctcgcc atgtcgctcg gcttcgccat gcgctggccg cgcctcgact
      601 gggacaaccc caagcagatg cgcaacggct gggctaacgt agcctcgctg gcgggtgacg
      661 cggtgctggg gctgctgggc ggcgggctgc tgctggtgcc gatcctggcc gagatctggg
      721 cgcccgcctg ggtgctgccc gcctggatca tcggcatcgc cggcgcggcc ggtgtcacgg
      781 tgggcctggc ctggacggcg ctcgccatcg gtctcgatca cctgccggag gtgggcgagg
      841 cctaatatcc aagggaacac gcggaccctg ccggcagaat ccggcagggt ccgcttattg
      901 gtgcctgctg caccccctgg gccgatccag ggctggcagg attatacccg gcgtttacca
      961 aaccggcact ctgcttttca ccgggccgcg ctatgctgta tggtgaggga gtctcgccgg
     1021 cgccgcccgc gtatgcaggc cgggtacccg gggccacgct tgcaacccgc tacggcagat
     1081 ttgcgtatag actcgcggaa cacctggtga cggtcccatg tcgcgcaggg cgcgctaccg
     1141 gaccgggccg cgccggcccc tggccggtgc tgggagatgt gggaggacac atgactatgg
     1201 ataacgaaac gcgccatctg gatcacgagg ccgcgcgcgc ggcgggtctg ccggcggacg
     1261 gcccatccgc gcccggcaac ccacccgccg ggaacggcgg cacgccgacc acctatctgc
     1321 cgccggtgac gaacgcggca actgcgtcgg aggccccgcg cccgagctac gcgccgccgg
     1381 ctaccggccc cacctatacg ccgcccgcgc cgggggcggt cccggcctac acgccgcccc
     1441 ggcccgccgg caacggccag gcgcggcgag gtagcgggcc ggagtcgctg atccccctgg
     1501 ccttgatcgc cgtgggcatc ctggcgctgt tcggcgggtt ccgctgggtc ttcggcgcgg
     1561 ccatcccgct ggcgctgggg ctgattttcc tgtatgtgag cagccagggg ccgggccgcc
     1621 tgggcttccg catccccggc tgcatcctga ccgggctggg cgcgggcatc gtgctcgaca
     1681 gcatgatcgg cggcggctgg tcggcggtgg gcctgggcct gggcttcgtc gccctctggg
     1741 tcatggaccg cgcgcagttg tggtggctga tccccggcgc cgccgtgggc ctgggcgggt
     1801 tgcagagcgt ctggagcggc agcgactggg gcggcaactg gttcttcccg ctggcgctga
     1861 tcgccttcgg cgcctatctg ttgagcgggc gccagtggcg cgctcggcgc taaacgcctc
     1921 catattactg ctactggccg tccggatcgt tgcgatccga gcggcttttt ttgcccacac
     1981 cccgccggga atcggccccg gcgctgtaac ctccatcggg ccgctcgcgt atatgtaacc
     2041 gatgacagat ccccaaggag atcggcagcc gggacgacct gcaggcgtct gctctgcccc
     2101 ttgcagtgga ccctacggag gaaacgaaat ggacaacgaa acccgccatc tggatgctcg
     2161 tggcgcgcgc gtgcccggcg cgatgccggc gcctccggcc ccgcccgcgc cgcccgtgtc
     2221 tgccggcgtc ccgacgagct atctaccggc catgcccgcg cc
//
LOCUS       DASUYC010000600         4069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1191502, whole genome shotgun sequence.
ACCESSION   DASUYC010000600 DASUYC010000000
VERSION     DASUYC010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191502"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VF276_09765"
     CDS             <1..216
                     /locus_tag="VF276_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607187.1"
                     /translation="APPDFAAVSVGLVVDEVHEIMSCPLAQLLPFPRWVVRDLPATTV
                     WAALPRDDSSLLLLLDGFALAAQQYQR"
     gene            226..2727
                     /locus_tag="VF276_09770"
     CDS             226..2727
                     /locus_tag="VF276_09770"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEX6607188.1"
                     /translation="MDDTRTALLGKFREEVDTHLLRLSQGVVALEQDPGNTGLLKEVF
                     RSAHTIKGAAKMMGFAEITRITHEMESVLGVMRDGQLQLTSDISDLLFEGVDAITLLT
                     QLQTSPGDAQATEALAELNSDQLVARLKAIADSAAPAAKEAPATPPVAPPAPSASNGT
                     ATALDHAEAAPPVAPYEAGPAGRGEAHAARADLTGFKPGEDSIRVRVQKLDTLMNLSG
                     EMVITKMQHEAIAGQIRTLLELERNRARFLADMRERVERSWGMINQVDMEEALEQLHN
                     LDTAIDDHTGSALREFQEYAGRLNSISDELQDTVLSVRMLPIETIFSTFPRAVRDFKR
                     ESGKEVEVLGRGGETELDKQILESLGDPLIHLLRNALDHGIESPAQRRAAGKPAQGLV
                     TVHAYAQGTQVLIEVSDDGAGMDPATIRRLAVQKNFISRLDAEKLSDEESLNLIFYPG
                     FSTASIITDVSGRGVGMDVVKTTVERMNGTVGIESQLGMGTRVTLRLPLTLATMQALL
                     VRVGTQVFVVPSHTVEGGMEYIGLEDIFMVEGHEVVRLKGRSMPLVRLADLLDLRRVE
                     SGTWLKDTGLQHLLHTPRPAAHNGHGPLDGEEEDLSLNFRAPVLGTGIRIENKLPGVI
                     VGSGDRQACFLVDELIDELDVVVKNLGPLLSKVETAIGATILGNGRVVVILDMPSLLA
                     EARNLSTRGQLGRAWQVAKAAKKPRILVVDDSITTRELEKSILENAGYEVEIAMDGLE
                     ALGKLEQQLQDGEQRYDLVIADIEMPRMDGLDLTQRVKTHQQDMLRNTPVIIVSSLAS
                     DAYKQRGIEVGAQAYITKGQFDQSHLLETIDLLIQ"
     gene            2808..3203
                     /locus_tag="VF276_09775"
     CDS             2808..3203
                     /locus_tag="VF276_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6607189.1"
                     /translation="MEKRRILVVDDSALVAEAVKAKLEASNYDVAVAHSGEEALEKVH
                     ESLPDLMILDVYMPGIDGFEVCRRLREDPATQSLPIVMLSSRGNIKEKLAGFRAGADD
                     YLVKEFDLLDLPYRVRLVFRLRGLETDRN"
     gene            3213..>4069
                     /locus_tag="VF276_09780"
     CDS             3213..>4069
                     /locus_tag="VF276_09780"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6607190.1"
                     /translation="MSQPIRVLIVEDSPLASEILTSILHSDPEIQVVGVARNGVEALE
                     QVPLLRPDLITMDVWMPRMDGFATVERLMAYYPTPILVITSSLARSDVDISLTMLAAG
                     ALDVIEKPSRGDEATWTRSTKALVQKVKTLARVRVVTHLKGRRPGTNPALGAGAAPGS
                     AGPPLVAPRTPPVPFTPPARPADRPLPTRHTDPETLAQRMAARRRTGTEPLLVLPPHP
                     APGSGHRYELVAIASSTGGPQALLKVLRGLPPTFAPPVLIVQHIAAGFTQGLADWLGR
                     EAGRAVRLAV"
BASE COUNT          751 a         1373 c         1338 g          607 t
ORIGIN      
        1 gcgccgcccg acttcgccgc cgtgtccgtt ggactcgtgg tcgacgaagt gcatgaaatt
       61 atgagttgcc cattggcgca actgttgccc ttcccgcgct gggtggtgcg tgacctcccg
      121 gcgacgacag tgtgggcggc attaccgaga gacgattcat ccttgctgct gctcctcgac
      181 gggttcgcgc tcgcggcgca gcagtaccag cggtaaggca ggaacatgga cgatacacgc
      241 acggcgctcc tcggtaagtt ccgcgaagag gtggacaccc acctcctgcg actgtcccag
      301 ggcgtcgtcg cgctggagca ggatccgggc aacaccggcc tactaaaaga ggtcttccgc
      361 tcggcgcaca ccatcaaggg cgcggccaaa atgatgggct tcgccgaaat tacgcgcatc
      421 acacacgaga tggagtcggt gctgggcgtc atgcgcgatg ggcaactcca actgacgagt
      481 gacatcagcg acctgctctt cgagggggtc gatgcgatca ccctgctcac gcaactccag
      541 accagtcccg gcgacgcgca ggccaccgaa gccctggccg agctgaactc cgaccaactg
      601 gtggcccggc ttaaggccat tgccgacagc gccgcgcccg ccgctaaaga ggccccggcg
      661 acgccgcccg tcgcgccccc ggcgcccagt gccagcaacg gcaccgccac ggccctggac
      721 cacgccgagg ccgcgccccc ggtggcgccc tatgaggcag gcccggcggg ccgcggcgag
      781 gcccatgcgg cccgcgccga tctcaccggg tttaagccgg gcgaagacag catccgcgtg
      841 cgcgtgcaga agctcgacac cttgatgaac ctcagcggcg agatggtcat caccaagatg
      901 cagcacgagg ccatcgccgg gcagatccgc accctgctcg aactcgaacg caaccgcgcc
      961 cgcttcctgg ccgacatgcg cgagcgcgtg gaacgatcct ggggcatgat caaccaggtc
     1021 gatatggaag aggccctgga gcagttgcat aacctcgaca cggccattga cgatcatacc
     1081 gggtcggcct tgcgcgaatt ccaggagtat gccgggcgcc tcaacagcat ctcggatgag
     1141 ctccaggata ccgtgctcag cgtgcgcatg ctgccgattg agaccatctt ctccaccttc
     1201 ccgcgcgcgg tgcgggactt caagcgcgaa agcggcaagg aagtcgaggt gctggggcgg
     1261 ggcggcgaaa ccgagctcga taaacagatc ctggagtcgc ttggcgatcc gctgatccac
     1321 ctgctgcgca acgcgctcga ccacggcatc gaatcaccgg cccagcgccg cgcggcgggc
     1381 aaacccgccc agggtctggt cacagtccat gcctacgctc agggcaccca ggtgctgatc
     1441 gaggtcagcg atgacggcgc ggggatggac ccggcgacga tccggcgcct ggccgtgcag
     1501 aagaacttta tcagccgcct ggacgctgag aagctgagcg atgaggaaag cctgaacctc
     1561 attttctatc ccggcttcag cacggccagc atcatcaccg acgtgtcggg ccgcggcgtc
     1621 ggcatggatg tggtcaagac caccgtcgag cggatgaacg gcacagtggg catcgagtcc
     1681 cagttaggca tgggcacccg cgtgacgttg cgcctgccgc tgacgttagc gaccatgcag
     1741 gccttgctgg tgcgcgtggg tacccaggtc tttgtggtgc ccagccacac cgtcgagggc
     1801 ggcatggagt acatcggcct ggaagatatc ttcatggtcg agggccacga ggtggtgcgg
     1861 ctcaaggggc gcagcatgcc gctggtgcgc ctggccgacc tgctggacct gcgccgcgtg
     1921 gaaagcggca cctggctcaa ggataccggg ctacagcact tgctgcatac gccgcgcccg
     1981 gcggcgcaca acggccacgg gcccctcgac ggcgaggaag aagacctgag cctgaacttc
     2041 cgcgccccgg tgctcggcac cggcatccga atcgaaaata agctgcccgg cgtgatcgtt
     2101 ggcagcggcg accgccaggc gtgcttcctg gtggatgaac tcatcgatga gcttgacgtg
     2161 gtggtcaaga atctgggacc attactgagc aaggtcgaga cggcgatcgg ggcgaccatc
     2221 ctgggcaacg gccgggtggt ggtcatcctc gatatgccga gcctgctggc cgaggcgcgc
     2281 aacctcagca cccgcgggca gcttgggcga gcctggcagg tggcgaaagc cgccaagaaa
     2341 ccgcgcatcc tggtggtcga cgactcgatc accacgcgcg aactggagaa gagcatcctc
     2401 gaaaacgccg ggtacgaggt ggagatcgcc atggacgggc tggaggcgct gggcaaactc
     2461 gaacagcaac tccaggacgg tgagcaacgc tacgacttgg tcatcgccga catcgagatg
     2521 ccgcgcatgg acggcctgga cctgacgcaa cgtgtgaaga cccaccagca agacatgctg
     2581 cgtaatacgc cggtgattat cgtctcgtcg ctggcgagcg acgcctacaa gcagcgcggc
     2641 atcgaagtgg gcgcccaggc ctatattacc aagggacaat tcgatcagag ccatctactt
     2701 gagaccatcg atttgttgat acagtaggat cgcgcggagc cggcccgccc cacgccgcag
     2761 cggcgggcgg acccactccc cgtgacccag ggatgaggat catcagcatg gaaaaacgcc
     2821 ggatattagt ggttgacgac agcgccctgg tggccgaggc cgtcaaagcc aagctggagg
     2881 ccagcaacta cgatgtagcg gtggcccaca gcggagagga ggcgctcgaa aaagtccacg
     2941 agagcctgcc cgatctgatg atactggacg tgtatatgcc cggcatcgac ggcttcgagg
     3001 tctgccgccg cctgcgcgaa gacccggcca cccagtcgct gcccatcgtc atgctcagtt
     3061 cgcgcggcaa catcaaggag aagctggccg gcttccgggc cggcgccgac gattacctgg
     3121 tcaaggaatt cgacctgctc gacctgccct accgcgtgcg cctggtgttc cggttgcgcg
     3181 ggctggaaac cgatcgcaac taaccgatcc ccatgagcca gcccatccgc gtcctgattg
     3241 tcgaagactc accgctggcg agtgagatcc tgacctccat cctgcacagc gaccccgaga
     3301 tccaggtcgt gggcgtggcc cgcaacggcg tcgaggcgct ggaacaggtg ccgctgctcc
     3361 gaccagacct gatcaccatg gatgtctgga tgccgcggat ggacggcttt gccacggtcg
     3421 agcggctgat ggcctattac ccgacgccga tcctggtcat cacctcatcc ctggcccgga
     3481 gcgatgtcga tatcagcctg accatgctgg ccgcgggcgc gctcgacgtg atcgagaaac
     3541 cctcgcgggg cgacgaggcg acctggacgc gctcgacgaa ggcgctggtg cagaaggtca
     3601 agacgctggc ccgcgtgcgg gtggtcacgc acctgaaggg ccgccggccc ggcacgaacc
     3661 cggccctcgg tgccggggcg gcgccgggca gcgccggccc gccgctggtc gcgccacgca
     3721 cgcccccggt cccgttcacg ccgccggccc gaccggccga ccgcccgctg cccacccgcc
     3781 acaccgaccc ggagacgctg gcccagcgca tggccgcgcg gcgccgcacg gggaccgagc
     3841 cgctgctggt gctgccgccc caccccgcgc cggggtccgg ccatcgctac gaactggtgg
     3901 cgattgcctc gtccaccggc ggcccccagg ccctgctgaa agtgctgcgc gggctgcccc
     3961 ccacgttcgc cccgcccgtg ctcatcgtgc aacacatcgc ggcgggcttc acccagggcc
     4021 tggccgactg gctgggtcgc gaggcggggc gggccgtgcg cctggccgt
//
LOCUS       DASUYC010000601         3326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_464225,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000601 DASUYC010000000
VERSION     DASUYC010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464225"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..2098)
                     /locus_tag="VF276_09785"
     CDS             complement(176..2098)
                     /locus_tag="VF276_09785"
                     /inference="COORDINATES: protein motif:HMM:NF020700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX6607191.1"
                     /translation="MGNRSSQNTKTGAANRVTNTGAGRSGGGGLRLFGQHTPNSIGSP
                     APGQAGHFADTWLSRLLIGGALLLLAIMFPLQWLRTPTLVVRANVGTFAPHPGVDPTA
                     ADGGPVTGAVTANYDLSEEASINAVVYDSAGAVVKTLLTAKQQSAGAHFILWDGSTDN
                     GSIAADGAYRLDITAKGPARSTSGSVTVTVDTTPPAVKLVNLTDGQKLKSNDLVVQGV
                     TEAGATVQFSDGGEVLTADSGGGFTLHRQLQHGTNTFKLLVRDSSGNETSVSRTVDLL
                     DQPPAIKIDTPADGAWTNQKLVTVSGTVDPGTTVRVNNQETAPGPDGRFHVDVVLDEG
                     ANNITVEATDPVGNKARVEQHATLDTRPPSVQIDSLADGTTVHEAQVHLYGRTDPGVK
                     LSIQGRQVGVDQQGRFDTPVDLVVGENTIHIEAQDQAGNVTPIDRKLRYDLGTSGFPD
                     LPITGTATALALIVLAWVIFGGWFSSVSLKLATDRPSFGPGRYSTGQADRLLITYSLS
                     KRARTTLQVVDSAGHVVTTLLRSSPRAAGDHQLLWDGGTLQGEFAAPGNYLIQAQART
                     ATSTVSASAPITITGRAAPRSVSVGTGGYGSYGPPAPGTPPPARYNYGYGQRGTATGG
                     TDPAPPERNVLRGDRG"
     gene            complement(2102..>3326)
                     /locus_tag="VF276_09790"
     CDS             complement(2102..>3326)
                     /locus_tag="VF276_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607192.1"
                     /translation="ERAVLAAGMAGAVLASQLLPNYPMEWRALLPLAVLGIGLWSTIG
                     AYLAMVAVLAYPIWQLSPYLMVLFVAVAIIPHRLILNHLPLALLIVWSPVLAALHVEF
                     AIPLLAGIFSGARRGAVAGLVTALWIKLYAAMSGNPLDLLLLQGSPGTPAGVAARFAG
                     AGSLDTVMLIGAPFAPNSSELLGNLLQIAAVAGAGAAAGWLAGWGRSRLVLHDLEDAP
                     PLWLRLAARVWWTGIVPVGVGAIVLGAALLLVPFAVGRPLSSLGLQNAANLLSMKLVL
                     SSAAVLGLIGLVLVAQPLPPSARRRPAAPRERINPAPPPVLPPPDLTPIKTGRFVPAW
                     AAQAGMEAPLPPATEPVDENDDLIDLLMAVPAASPADDGDGPAGAMRERTGSAGAGVT
                     PAPGPPPREIAIELD"
BASE COUNT          471 a         1140 c         1234 g          481 t
ORIGIN      
        1 aggccgggaa gggccgcccg ctcaggaagc gggcggcgat gggcagcgcg gcgtcgtcca
       61 gcgcgcccag gtaggcgccg aggatggcgg ctttttccag gcgcttggac gtggcgcccg
      121 cggcatccgc cgtggcgacg agatcgtgaa agggcgcgtc cgccatgcgg gccggttagc
      181 cccggtcgcc gcgcagcacg ttgcgctcgg gcggcgcggg atccgtgccc ccggtcgcgg
      241 tgccgcgctg gccgtagccg tagttatagc gcgcgggcgg cggggtgccg ggcgcgggcg
      301 ggccatagct gccgtagccg ccggtaccca cgctaacgct acgcggcgcc gccctgccgg
      361 tgatggtgat cggcgcgctg gcgctgactg tcgacgtggc ggtgcgggcc tgggcctgga
      421 tcaggtagtt gcccggcgcg gcaaattcgc cctgcaacgt gccgccgtcc cacagcaact
      481 ggtggtcgcc cgcggcgcgc ggcgaggagc gcagcagcgt cgtgacgacg tgcccggcgc
      541 tgtccaccac ttgcagcgtg gtgcgcgccc gcttcgagag cgagtaggta atcagcaggc
      601 ggtcggcctg cccggtgctg tagcggcccg gcccgaaaga cggccggtcg gtcgccagct
      661 tgagcgagac cgacgagaac cagccgccga agatcaccca ggccagcaca atcagcgcca
      721 gcgccgtggc cgtgccggtg atcggcaggt cggggaagcc ggacgtgccc aggtcgtagc
      781 gcagcttgcg gtcgatgggt gtcacgttgc ccgcctggtc ctgggcctcg atgtgaatcg
      841 tgttctcgcc caccaccagg tctaccggcg tatcgaagcg cccctgctga tcgacgccca
      901 cctgccggcc ctggatcgac agcttcacgc cgggatcggt gcgcccgtag agatgcacct
      961 gggcttcgtg gaccgtggta ccgtcggcca ggctgtcgat ctgcacactg ggcgggcgcg
     1021 tatccagcgt ggcgtgctgc tcgacccgcg ccttgttgcc gaccgggtcg gtggcctcca
     1081 cggtgatgtt gttcgcgccc tcgtccagca ccacatcgac gtggaagcgg ccgtcggggc
     1141 caggtgccgt ctcctggtta ttgacccgca cggttgtgcc ggggtccacg gtgccggaga
     1201 cggtgaccag tttctggttg gtccaggcgc cgtcggcggg cgtgtcgatc ttgatcgccg
     1261 ggggctggtc gagcagatcg acggtgcggc tgacgctggt ctcgttgccg ctgctgtcac
     1321 gcacgagcag cttgaacgtg tttgtcccgt gctgcaactg gcgatgcagg gtgaagccgc
     1381 cgccgctgtc tgcggtcagc acttcgccgc cgtcggagaa ctggacggtg gcccccgcct
     1441 cggtgacgcc ctggaccacc aggtcgttgc ttttcagctt ctgcccgtcg gtcaggttaa
     1501 ccagcttgac cgcgggcggc gtggtatcca ccgtcacggt gacgctgccg ctggtcgagc
     1561 gggccgggcc tttggctgtt atgtcaagac gataagcgcc gtcggcggcg atgctgccgt
     1621 tgtcggtgga gccgtcccag aggatgaagt gggcgccggc gctctgctgc ttggcggtca
     1681 gcagggtctt gacgacggcg cccgcgctgt cgtagaccac ggcgttgatg ctggcttctt
     1741 cggacaggtc gtagttggcc gtgaccgccc ccgtgaccgg gccgccgtcc gccgccgtgg
     1801 gatcgactcc tggatgcggg gcgaaagtgc cgacattggc gcgcaccacc agggtgggcg
     1861 tgcgcagcca ttgcagcggg aacatgatgg cgagcagcag caaggcgccg ccgatgagca
     1921 agcgcgacag ccaggtgtcg gcgaagtgtc cggcctgacc cggcgcgggc gaaccgatgc
     1981 tgttgggggt gtgctggccg aagaggcgca acccccctcc gccggagcgg ccggcgccgg
     2041 tgttcgttac gcggttggcg gcgccggtct tcgtattctg gctggatcgg ttgcccatgg
     2101 gtcagtccaa ttcaatcgcg atttcgcgcg gcggcgggcc gggggcgggc gtcacacccg
     2161 cgccggcact accggtccgt tcgcgcatcg cgccggcagg cccatccccg tcgtccgcgg
     2221 gcgaagcggc gggcaccgcc atcagcaagt caatcaggtc gtcgttctcg tccaccggct
     2281 cggtggcggg cggcaggggc gcctccatcc cagcctgagc cgcccaggcg ggcacgaagc
     2341 gcccggtctt gatcggcgtg aggtcgggcg gcggcagcac gggcggcggc gccgggttga
     2401 tccgctcgcg aggtgccgca ggccggcggc gcgcgctcgg cggcagcggc tgggccacca
     2461 gcaccagccc gatcaggccc agcacggcgg cgctgctcag cacgagcttc atgctcagca
     2521 agttggcggc gttttgcagg cccaggctgc tcagcgggcg gcccacggcg aacggcacca
     2581 gcagcaaggc cgcgcccagc acaatcgccc ccacacccac cggcacgatg ccggtccacc
     2641 agacgcgggc ggccaggcgc agccagagcg gcggcgcgtc ttccaggtcg tgcagcacca
     2701 ggcgcgagcg gccccacccc gccagccagc ccgcggcggc ccccgccccg gccaccgccg
     2761 cgatctgcag caggttcccc agcaactcgc tggaattggg agcaaagggc gcgccgatga
     2821 gcatgaccgt gtccagcgac ccggcgccgg cgaaccgcgc ggccacgccc gcgggcgtac
     2881 cggggctgcc ttgcagcaag agcaggtcga gtgggttgcc gctcatggcg gcgtagagct
     2941 tgatccagag cgccgtcacc agcccggcca cggcgccgcg ccgcgcgccg gagaagatcc
     3001 ccgccagcag cgggatggcg aactcgacat gcagggcggc cagcaccgga ctccagacga
     3061 tcagcaacgc cagcggcagg tggttcagga tcagccggtg cgggatgatc gcgacggcga
     3121 cgaacagcac catcaggtac ggcgagagtt gccagatggg ataggccagc acggcgacca
     3181 tcgccaggta ggcgccgatg gtgctccaga ggccgatgcc aagcacggcc agcggcagca
     3241 gcgcccgcca ctccatgggg tagttgggca acaactgcga ggccaggacc gccccggcca
     3301 tgccggcggc caggacggcg cgctcc
//
LOCUS       DASUYC010000602         5436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1179281, whole genome shotgun sequence.
ACCESSION   DASUYC010000602 DASUYC010000000
VERSION     DASUYC010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5436
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179281"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..592)
                     /locus_tag="VF276_09795"
     CDS             complement(74..592)
                     /locus_tag="VF276_09795"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX6607193.1"
                     /translation="MEIILMAAVTLDGKLARHAAHLSDWTSPEDKKLFRTETRRAGVI
                     ILGHNTFRTLARPLPGRLHIVLTRDRSDKVDIPGVVEYTSAPPATIAADLAARGYTRA
                     ILVGGAQANALFLAADLVDEIWLTVEPVIFGEGLSLFAGMAFDRRARLVSLTPLNADA
                     FLARYSLRPSSE"
     gene            complement(601..1764)
                     /locus_tag="VF276_09800"
     CDS             complement(601..1764)
                     /locus_tag="VF276_09800"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX6607194.1"
                     /translation="MAEPGQDGVLIIGGGIGGLAAGIALHRAGIPAQVLEQRAAAGEL
                     GAGLTLWANAMHALARLDLADTVRARGRAGIGGTIRTARGAPLQHITPAALERAFGSA
                     NVAIHRADLQVLLMAALPAGTLHLGMRLTGVEQDGAGVRACFANGQEARGAVLIGADG
                     IRSTVRAGLWGDAPPRYAGYTARRGFARIARDPPPGAEAGFEAWGCGARLGAVAIGGR
                     RVYWFATRNAPEGETDSPRGRKADVLDLLAGWHPTMLALVAATDEAAILRHDIYDRPP
                     RAPWGQGRVTLLGDAAHAMTPNLGQGACQAIEDAVVLADVLRAADDPVAGLRAYEAAR
                     APRTAQIVRQSWLMGRIGQWEQPGLCRVRAAVMRAMPERVVLAQLAPVLGYRV"
     gene            complement(1767..4928)
                     /locus_tag="VF276_09805"
     CDS             complement(1767..4928)
                     /locus_tag="VF276_09805"
                     /inference="COORDINATES: protein motif:HMM:NF023218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX6607195.1"
                     /translation="MLHGVSFRLLHNPPIDAIQALFTRLRQDGCDAVSIIPHHYVSLV
                     PGTANLGPPPKEFGIPWFIYPDVGQDPAHPFHNTPEPELVLETCQLAAKLGFRVMLKP
                     HVDSYQAGWRGNISVKDHADDWSWGYRNRFLQRYVDIAKQVNGTILCLGCELYTVTKE
                     LGPDFWIGVADWVRGQGFQGPLTYAANWGWADDAEYRRLAPLWPHLDYIGVDAYFPLI
                     AQGAGGATDAGALAQAWHRKGIDADWCPCIDDDLEALSKSAGKPLLFTEIGYGNHQQA
                     AIDPARDPLPTDVRDDDLQLQLGQAFRQRWGGVPGFAGYFWWEAFLDQGDRPEISHDI
                     IDRPIEKIIFQPLPGETATPTPVPTPVPQPVPTPVPPPVVPPVPPPVVEPQPTPEQVL
                     ALNGLHGPNCAFTAFHTQPRGKAEGADSAWQLVGAAHATWIKLLWPDHHRADAERARA
                     MGLKVLVRAPGEGYPASRDVQAMIQEFRGVCDVIEVGNEPAPNSKYATWDQAYWNHAW
                     YVENVWQQCSDAAHAAGILLCAPGWQAEKEPPSPDSVGSQALSDRLHQVYGAYDAVAV
                     HCYDSFNLDIPPVIDRIARWYSAFNKPVYITEYGIAARVLPGAGATDSDAVKVQRYVA
                     FLRRLATRDYIKAAFLFILNGTDDFACFNAGGYDPNGANSYWLNPAAYALLGNTLSSA
                     SRDLEPLPPLDRGLGGDLSQGLAVHIGADDRPHGPDYPIQSVPTISPAVYASVLQAHK
                     SPALQEADGLDYYRAATAKGINPAVALAFFEHESVCGTQGPVVDAGAKNWGNLRPRND
                     GTIGRAVRKVSTQYGVFRGYNRYLDGLLDWCDLMLSVYQGMSIRQALKIYAPASDQND
                     PNSYAGIVLGRLAKWDQASGDFDVPGTAPVPAPAPTPAPAPAPVAPPAQNGPTDYPLL
                     AAPTISSAAFTAALAGSPALDEAPGEAYYNLCVSNDVDPAVALAFFQQESNCGLLPGA
                     AEHKNWGNLWDAQANALGTYGTWLDGLRDWCARLQRPAYTAHGTPTIASIVPLYQPAG
                     AKQNGNDWYIARLSARIAGMQGT"
     gene            complement(5254..>5436)
                     /locus_tag="VF276_09810"
     CDS             complement(5254..>5436)
                     /locus_tag="VF276_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009784606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX6607196.1"
                     /translation="DWWMGYVEEVPGANTQGATLEEARENLKDAVQLAMAAHRELAQR
                     ETEGKTVIREELRVGV"
BASE COUNT          796 a         1844 c         1940 g          856 t
ORIGIN      
        1 caacaacatg cgtctgatct tcagcgaggc caacctgcgc gccctgacgc aaccccagcg
       61 gcagtaagcg ccgtcattcc gaacttggcc gcaggctata gcgcgccagg aacgcgtccg
      121 cattcagcgg cgtcaggctg acgaggcggg cgcggcggtc gaacgccatc cccgcgaaca
      181 ggctcagccc ttccccgaaa atgaccggct cgacggtgag ccagatttcg tccaccagat
      241 cggcggccag gaagagcgcg ttggcctgcg ccccccccac cagaatcgcg cgtgtgtagc
      301 cgcgcgcggc aaggtccgcc gcgatggtgg ccgggggtgc actggtgtat tcgaccacgc
      361 cggggatgtc caccttatcg ctgcggtcgc gggtcagcac gatgtgcagg cggcccggca
      421 gcgggcgggc tagggtgcgg aaggtgttgt ggcccaggat gatcaccccg gcgcggcgcg
      481 tctcggtccg gaacagcttc ttatcttcgg gcgaggtcca gtccgacagg tgggccgcgt
      541 gacgggccaa cttgccgtcc agcgtcaccg ccgccatgag gataatttcc atggccctgg
      601 ttatacccgg tagcccagga cgggcgccaa ctgggccagc accacgcgct cgggcatggc
      661 gcgcatcacg gcggcgcgca cccggcagag gcccggctgc tcccactggc cgatgcggcc
      721 catcagccac gactggcgca cgatctgggc ggtgcgcggg gcacgggcgg cttcgtaggc
      781 gcgcagcccg gccaccgggt cgtccgcggc acgcagcaca tcggccagca ccacggcgtc
      841 ttctatggcc tggcaggcgc cctgccccag gttgggcgtc atcgcgtggg cggcgtcgcc
      901 cagcagcgtg acccggccct gcccccaggg ggcgcggggc ggccggtcat agatgtcgtg
      961 gcgcaggatc gccgcctcgt cggtcgcggc gaccagggca agcatggtcg ggtgccagcc
     1021 ggccagcagg tcgagcacgt cagccttgcg cccgcgcggg gagtcggttt cgccctccgg
     1081 cgcgttgcgc gtggcgaacc agtagacccg ccgcccgccg atagccaccg cgcccaggcg
     1141 cgcgccgcag ccccacgcct cgaacccggc ctccgcgccc ggcggcgggt cgcgagcgat
     1201 gcgggcgaac ccgcgccggg ccgtataccc ggcatagcgc ggcggcgcat cgccccataa
     1261 cccggcgcgc accgtcgagc ggatcccgtc cgcgccaatc aacacggcgc cgcgggcttc
     1321 ctgcccgttg gcgaaacagg cgcgcacgcc cgcgccgtcc tgctccacgc ccgtcagccg
     1381 catgcccagg tgcagcgtgc cggcgggcag cgccgccatc agcaggacct gcaagtcggc
     1441 gcggtggatc gccacgttgg ccgagccgaa ggcgcgctcc agggcggcgg gcgtgatgtg
     1501 ttgcagcggc gcgccccgcg ccgtgcggat ggtgccgccg atcccggccc ggccccgcgc
     1561 ccgcacggta tccgccaggt ccagccgggc gagcgcgtgc atggcgttgg cccacagggt
     1621 cagcccggcg cccagttcgc ccgccgcggc ccgctgctcc aacacctggg cggggatgcc
     1681 tgcccggtgc agcgcgatgc ctgccgccag cccgccgatc cccccgccga tgatcaagac
     1741 gccatcctgc cccggttccg ccatgctcac gtcccctgca tgccggcgat gcgcgcgctg
     1801 agccgggcga tataccagtc attgccgttc tgcttggccc cggcgggctg gtagaggggc
     1861 acaattgagg cgatggtcgg cgtaccgtgg gcggtatagg ccgggcgttg caggcgcgca
     1921 caccaatcgc gcagcccgtc gagccacgtc ccataggtgc ccagcgcgtt cgcctgggcg
     1981 tcccacaggt tgccccagtt cttgtgctcg gcggcgccgg gcagcaggcc gcagttgctc
     2041 tcctgttgga agaaggccag ggccacggcg gggtccacgt cgttgctcac acagaggttg
     2101 tagtaggctt cgcccggcgc ctcgtccagc gcggggctgc ccgccagcgc ggcggtaaac
     2161 gcggcggagc tgatcgtcgg cgcggcgagc aaggggtaat cggtggggcc gttttgcgcg
     2221 ggcggcgcga caggcgcagg cgccggggcc ggggtagggg caggcgcggg cacgggcgcc
     2281 gtgcccggca cgtcgaagtc gccgctggcc tggtcccact tggcgagacg gcccagcacg
     2341 atgccggcgt agctgttggg gtcgttctgg tccgaggcgg gcgcgtagat cttgagcgcc
     2401 tgacggatcg acatgccctg gtagaccgag agcatcaggt cgcaccaatc cagcaagcca
     2461 tccaggtaac ggttatagcc acggaacacg ccgtattgcg tgctcacttt acgcaccgcc
     2521 cggccaatag tgccgtcgtt gcgcgggcgc aggttgcccc agttcttcgc ccccgcgtcc
     2581 accactggtc cctgggtgcc gcagacgctt tcgtgctcga agaacgccag cgctaccgcc
     2641 gggttgatgc ccttggccgt ggccgcacgg tagtagtcga gcccatcggc ttcctgcaac
     2701 gccggactct tgtgcgcctg cagcaccgag gcgtacacgg cgggactgat ggtgggcacg
     2761 ctctggatcg ggtagtcggg gccgtgcggc cggtcgtccg cgccgatatg cacggccagc
     2821 ccctggctca ggtcgccgcc caggccgcgg tcaagcggcg gcagcggctc caggtcgcgc
     2881 gaggcgctgc tcagggtatt gccgagcagg gcataggccg ccgggttcag ccagtagctg
     2941 ttggcgccgt tcgggtcgta gcccccggcg ttaaagcagg caaagtcgtc ggtgccgttg
     3001 aggatgaaca ggaacgccgc cttgatgtaa tcgcgcgtcg ccagccgccg caggaaggcg
     3061 acatagcgct gcaccttcac ggcgtcgcta tcggtggcgc ccgcacccgg cagcacccgc
     3121 gccgcgatgc cgtactcggt gatgtagacc ggcttgttga acgcgctata ccagcgggcg
     3181 atacggtcga tcaccggcgg gatgtcgagg ttgaacgagt cgtagcaatg gacggcaacg
     3241 gcatcgtagg cgccatagac ctggtgcagg cggtcggaca gggcctggct gccgaccgag
     3301 tcggggcttg gcggctcttt ctcggcctgc cagcccggcg cgcagagcag aatcccggcg
     3361 gcgtgcgcgg catcggagca ttgctgccag acattctcca cgtaccaggc gtggttccag
     3421 taggcctggt cccaggtggc gtacttgctg ttgggcgccg gctcgttgcc cacctcgatc
     3481 acgtcgcaga cgccgcgaaa ctcctggatc atggcctgca cgtcgcgcga ggcgggatag
     3541 ccttcgccgg gggcgcgcac gagcaccttg aggcccatgg cgcgggcgcg ctcggcatcg
     3601 gcgcggtggt ggtcgggcca gagcagcttg atccaggtgg cgtgcgccgc gccgaccagt
     3661 tgccaggcgc tatcggcgcc ctcggccttg ccgcgcggct gggtgtggaa cgcagtgaag
     3721 gcgcagttcg gcccatgcag cccgttcagg gccagcacct gctccggcgt gggctgtggc
     3781 tccaccacgg gcggcgggac cggcggcacc acgggcggcg gcacgggagt cggcaccggc
     3841 tgcggcaccg gagttgggac cggcgtgggg gtggccgtct cgccgggcag cggctggaag
     3901 atgatcttct cgatgggccg gtcgatgatg tcgtgactga tctcggggcg gtcgccctgg
     3961 tcgaggaagg cttcccacca gaagtacccg gcaaagcccg gcactccccc ccagcgctgc
     4021 cggaacgcct gccccagttg cagttgcagg tcgtcgtcgc gcacgtcggt cggcaacggg
     4081 tcgcgggcgg ggtcgatggc ggcctgctgg tggttgccgt agccgatctc ggtgaacagc
     4141 agcggcttgc ccgcgctctt gctcagcgcc tccaggtcgt cgtcgatgca ggggcaccaa
     4201 tcggcgtcga tgcccttgcg gtgccaggcc tgggcgagcg ccccggcgtc tgtcgctccg
     4261 ccggcgccct gcgctatgag cgggaaatag gcgtccacgc cgatgtagtc gaggtgcggc
     4321 cagagcggcg ctagccggcg atactccgcg tcgtcggccc agccccagtt ggcggcgtag
     4381 gtcagcggcc cctggaagcc ctgtccgcgc acccagtcgg ccacgccgat ccagaagtcc
     4441 gggcccagct ccttggtgac ggtgtagagt tcgcagccca ggcagaggat ggtgccgttc
     4501 acctgcttgg cgatatcgac gtagcgttgc aggaaccggt tgcgatagcc ccaggaccag
     4561 tcgtccgcgt ggtccttgac cgagatattg ccccgccacc cggcctgata ggagtcgacg
     4621 tgcggcttga gcatgacgcg gaagccgagt tttgcggcca gttggcaggt ttccagcacc
     4681 agttcgggct ccggcgtgtt gtggaagggg tgtgccgggt cctgccccac atcggggtaa
     4741 atgaaccagg ggatgccgaa ctccttgggc ggcggcccca ggttcgctgt ccccggcacc
     4801 agcgagacgt agtgatgcgg gatgatcgac acggcgtcgc acccgtcctg gcgcaagcgg
     4861 gtaaacagcg cctggatcgc atcgatgggc ggattatgca acaggcggaa cgaaacacca
     4921 tgcaacacgg gtggatctcc tcactgatgg gggaaacagg tcacgcggtc ccggtatctc
     4981 aggtgtggat cggtgcttca gcaagcggtt gcgacggcta cgcggcgggg cgagaggcag
     5041 ggcgtcttag cgtacccgtc cgttgccggt attatagcac aggagggggc agaggttatg
     5101 ggttccactc acggcgcggg gatgtgggag tcttggcaaa ttttacgcca gtttctgtgg
     5161 gtcgcgggtc ggctagtccg ctaggccgat tttgcgttgt aggggccggt ttcaaaccgg
     5221 cctgccgccc agcagaatca gagcggggaa cagctaaact cccacgcgca attcttcgcg
     5281 aatgaccgtc ttaccctccg tttcgcgctg agccagttcc cggtgtgctg ccatcgcgag
     5341 ttgcacggcg tctttcaaat tctcgcgcgc ttcttcgagc gtggctccct gggtattggc
     5401 gcccggcacc tcttcaacat accccatcca ccagtc
//
LOCUS       DASUYC010000603         2394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_293603,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000603 DASUYC010000000
VERSION     DASUYC010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293603"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1211)
                     /gene="clpX"
                     /locus_tag="VF276_09815"
     CDS             complement(<1..1211)
                     /gene="clpX"
                     /locus_tag="VF276_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEX6607197.1"
                     /translation="MAGTRGGKPQYHCSFCGKSQDDVRRLIAGPNSGYICDECVDLCR
                     EIIDEEETSVQKPEVNIGRTILKPKRIYELLNQYVIGQERAKKVLSVAVYNHYKRIGS
                     NNRGDDVELQKSNILLLGPTGSGKTLLAQTLAKILDVPFSIADATALTEAGYVGEDVE
                     NILLRLLQAADLDIARAEKGIVYIDEIDKIARKSDNPSITRDVSGEGVQQALLKIIEG
                     TVANVPPQSGRKHPHQDFIQIDTSNILFICGGAFEGLEEIISRRVGGGRAMGFQRGAD
                     PDKPEADVMSQVIPEDLLKYGLIPEFVGRLPMVVSLNPLDKATLMRILTEPKNAVIKQ
                     YQKFFALDRVELAFSTDALEAVADRALDLKTGARGLRSTIDEVLLDIMYELPSMEGVR
                     RCVVTGEVIQHG"
     gene            complement(1369..2055)
                     /locus_tag="VF276_09820"
     CDS             complement(1369..2055)
                     /locus_tag="VF276_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEX6607198.1"
                     /translation="MDRSFSPQAIVPYVVESTNRGERVYDIYSRLLRDRIIFIGTPID
                     DNIANIVVAQLLFLDYEDPERDVQLYIHSPGGSVTAGLAIYDTMQFIRPDVSTICIGS
                     SASMATILLCAGTKGKRLALPNSTIHMHPAGVGQIGGYAPDVEIQARELLRMQSKIRE
                     IMSKHTGQPLERIAHDFDRDLYMDSQQALDYGIIDEVVTSMAAISSDAAEGVKNEDAK
                     SDGKKKESVA"
     gene            complement(2185..>2394)
                     /locus_tag="VF276_09825"
     CDS             complement(2185..>2394)
                     /locus_tag="VF276_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607199.1"
                     /translation="GTEEPVASSDAGVATGSAVPSSDATQVAPSGAVPATAQEPGPLE
                     TAGGAAEVLGTGEAVDAERAPGAPE"
BASE COUNT          369 a          753 c          807 g          465 t
ORIGIN      
        1 ccatgctgga tcacctcgcc ggtcacgacg cagcggcgca cgccttccat cgagggcagt
       61 tcgtacatga tgtcgagcaa cacctcgtcg atggtgctgc gcaagccgcg ggcgcccgtc
      121 ttgaggtcca gcgcgcggtc ggccacggct tcgagcgcgt cggtgctgaa ggccagttcc
      181 acgcggtcga gggcgaagaa cttttggtac tgcttgatca cggcgttctt cggctcggtc
      241 aggatgcgca tcagcgtggc cttgtccagc gggttgagcg acacgaccat gggcaggcgc
      301 ccgacgaact cagggatcag gccgtatttc agcaggtcct ccgggatcac ctgggacatc
      361 acgtcggcct cgggcttgtc ggggtccgcg ccgcgctgga agcccatggc ccgcccaccg
      421 ccgacgcgcc gcgagatgat ctcctccagc ccctcgaagg cgccgccgca gatgaagagg
      481 atgttgctgg tgtcgatctg gatgaagtcc tggtgcgggt gcttgcgccc gctctggggc
      541 ggcacattgg cgaccgtgcc ctcgatgatc ttgagcagcg cctgctgcac gccctcgccc
      601 gacacgtcgc gggtgatcga cgggttgtcg ctcttgcgcg cgatcttgtc gatctcgtcg
      661 atgtagacga tgcccttctc ggcgcgggca atatcgagat cggcggcctg gagcaggcgc
      721 agcaggatgt tctccacgtc ttcgccgaca tagccggcct cggtcagcgc cgtagcgtcg
      781 gcgatggaga acggcacgtc gaggatcttc gccagggtct gggccagcag ggtcttgccg
      841 gagccggtgg ggccgagcag caggatgttg ctcttctgca actccacatc gtcgccgcgg
      901 ttgttgctgc cgatgcgctt gtagtgattg tagacggcca cggagagcac cttcttggcg
      961 cgttcctggc cgatcacata ctggttcagc agttcgtaga tgcgcttggg tttgaggatg
     1021 gtgcgcccga tattgacttc gggcttctgg acgctggttt cctcttcgtc gataatctcc
     1081 cggcagaggt ccacgcactc gtcgcaaata tagccggagt tcgggccggc gatgaggcgc
     1141 cggacatcat cctggctttt gccgcaaaag gagcaatgat actggggttt gccgccgcgg
     1201 gtaccggcca tgctgtctcc ctctcttcat acacgccacg ccggggcgaa aatcgggacc
     1261 cctgccgggg tcaacaggtc ggtgggccgc ccgtggcccg cacggggcgg cggatagggt
     1321 aggggccacg cggtggcggc ccctaccccg tcgtggagcc gggcctggtt aggccacgct
     1381 ctcctttttc ttgccgtcgc tcttggcgtc ctcgttcttg acgccctcgg cggcgtcgct
     1441 gctgatcgcg gccatgctgg tcacgacctc gtcgatgatg ccgtagtcga gggcctgctg
     1501 cgaatccatg tagaggtcgc ggtcgaagtc gtgggcgatg cgctccagcg gctggccggt
     1561 gtgcttggac atgatctcgc ggatcttcga ctgcatgcgc agcagctcgc gcgcctggat
     1621 ttcgacatcg ggcgcgtagc cgccgatctg gccgacgccg gccgggtgca tgtggatcgt
     1681 cgagttgggc aaggccaggc gcttgccctt ggtgcccgcg cagagcagga tggtcgccat
     1741 cgaggccgag ctaccgatgc agatggtgga cacatccggg cggataaact gcatcgtgtc
     1801 gtagatggcc aggccggcgg tcacggagcc acccggactg tggatgtaca actgcacatc
     1861 ccgctccggg tcttcatagt ccaggaacag caactgcgcg acgacaatat tagcgatatt
     1921 atcgtcgatg ggcgtgccga tgaagatgat ccggtcgcgc aacaggcgcg agtagatatc
     1981 atagacgcgc tcgccccggt tcgtgctctc gaccacataa gggacgatcg cctgcggcga
     2041 gaaagaccga tccattgtat gcctcctata gggctagcca ttattagtat accgcaacaa
     2101 ctgccatata gcaataatgt atccagccca tttgttcgat tttgcatggg tgggcgccat
     2161 gcgtcagcgg ggcggcggcc cgcgttactc gggggcgccc ggcgcgcgct cagcgtcgac
     2221 ggcctcgccg gtgccgagca cttcggcggc gccaccggcg gtttccagcg gccccggttc
     2281 ctgcgcggtc gcggggacgg cgccgctcgg cgcaacctgc gtggcgtccg acgacggcac
     2341 cgcgctgccg gtggcgactc cggcgtccga cgacgcgacg ggctcctccg tgcc
//
LOCUS       DASUYC010000604         2264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1447289, whole genome shotgun sequence.
ACCESSION   DASUYC010000604 DASUYC010000000
VERSION     DASUYC010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447289"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VF276_09830"
     CDS             <1..690
                     /locus_tag="VF276_09830"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6607200.1"
                     /translation="AHLPFFVERYHCLVYDCRGQGQSDKPDQVYETQLHTADLAALVE
                     ALGIEQAFFVGLSNGGAALLGYAAAHPARVQAQVVSGAYAAVDTILHVKLTSWIRAME
                     VGGSPLRFDVALPWVWGGGYLEQNYTGLLAFREKGVNLPIAPATNLIKGAMVHDIRAQ
                     LPAITAPTLVVVGEDDVLTPPKLARDIVAAVPGARLRVMDGLGHAAALEQPEAFCRIV
                     TEFFAQVAERN"
     gene            694..1473
                     /locus_tag="VF276_09835"
     CDS             694..1473
                     /locus_tag="VF276_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6607201.1"
                     /translation="MPFVRAGDLNVHYIEQGAGTPIVFVHGNWVTSTTWEPVMNRLPP
                     GYHGIAPDVRGRGKTEGPDSDYSIPSLAADLRAFVDALGLDRFHLVGHSLGTAIAMQF
                     ALDHPERVRTLTVVAPAWVDGMPEAFNNPAGQEALKANKALFGQALKALVPAAPADAY
                     WQRVVDEGHEQRLEASLRNVQACLDWKPGDRVGEAGIPTLVVDGAQDILTGGPNIDRA
                     AAALKARKTVVLPDIGHSPNMEAPDAFVALLIEHIKESENG"
     gene            1466..>2264
                     /locus_tag="VF276_09840"
     CDS             1466..>2264
                     /locus_tag="VF276_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6607202.1"
                     /translation="MAEESLLPGITSVTVPASRLQMRVLTSGPDDGVPVVLVHGNTSA
                     ARFWEETMLALPAPYRALAPDLRGYGQTEALPLDATRGLRDWADDLHALVKALALGPA
                     HWVGWSMGGGVVMQYAIDHPADVRSLTLVDPLAPYGFGGTKDTAGTPTYPDFAGSGGG
                     TANPEFVKLLREGDRSADSPTSPRNVMNTFYFKPPFKAAPDREEVFVTEMLSMRIGDD
                     FYPGDMTTSENWPGVAPGPRGINNAMAPGYCDRSGFAQVTPQPPVLWV"
BASE COUNT          352 a          794 c          776 g          342 t
ORIGIN      
        1 gcccacctgc ccttcttcgt ggaacgctat cattgcctgg tctacgactg ccggggccag
       61 ggccagtcgg acaaacccga tcaggtctac gagactcaac tgcacaccgc cgatctggcc
      121 gcgctggtcg aggcgctggg gatcgagcag gccttcttcg tcggcctgtc gaatggcggc
      181 gcggccctgc tgggctacgc cgccgcgcac ccggcgcgcg tgcaggcgca ggtcgtgtcg
      241 ggcgcctatg ccgcggtcga tacgatcctg cacgtcaagc tgacttcctg gatccgggcg
      301 atggaggtag gcggcagccc gctgcgcttc gacgtggccc tgccctgggt ctggggcggc
      361 ggctacctgg agcagaacta caccgggctg ctggccttcc gcgagaaggg cgtcaacctg
      421 cccatcgccc cggccaccaa cctgatcaaa ggggccatgg tccacgatat ccgcgcccaa
      481 ctcccggcga tcaccgcgcc gacgctggtc gtggttggcg aggacgacgt gctgacgccg
      541 cccaagctgg cgcgcgacat cgtcgcggcc gtgcccggcg cccggttgcg cgtgatggac
      601 gggctgggcc acgcggcggc cctggaacag ccggaggctt tttgccgcat cgtcaccgaa
      661 ttcttcgcgc aggtcgcgga aaggaactaa gtcatgccgt ttgtgcgcgc cggggatctg
      721 aacgtccact atatcgaaca gggcgcgggc acgcccatcg tctttgtcca cggcaactgg
      781 gtgacatcga ctacctggga gccggtgatg aaccggctgc cgcccggcta tcacggcatc
      841 gcgcccgacg tgcgcgggcg cggcaaaacc gagggcccgg attccgacta cagcatcccc
      901 tcgctggccg ccgacctgcg cgcctttgtc gatgcgctgg ggctggaccg gttccacctc
      961 gtcgggcact cgctgggcac ggccatcgcc atgcagttcg cgctcgacca tcccgagcgc
     1021 gtgcgcacgc tcaccgtggt cgccccggcc tgggtagacg gcatgccgga ggcgttcaac
     1081 aacccggcgg gccaggaagc cctcaaggcc aataaggcgc tgttcgggca ggcgctcaag
     1141 gcgctggtcc cggcggctcc cgccgacgcc tactggcagc gcgtggtcga tgaggggcac
     1201 gagcagcggc tggaagcgtc gctgcgcaat gtgcaggcct gcctggattg gaagccgggc
     1261 gacagggtgg gcgaagcggg catccccacg ctggtggtag acggcgcgca ggacatcctg
     1321 accggcgggc cgaacatcga ccgcgccgcc gccgcgctca aagcccgcaa gacggtggtg
     1381 ctgccggata tcggccactc gccgaacatg gaagcccccg atgcctttgt ggcgctgtta
     1441 atcgagcata tcaaggagtc cgaaaatggc tgaggaatcg ctgttacccg gcatcacgtc
     1501 cgtgaccgtg ccggcgagcc gcctgcagat gcgcgtgctg accagcgggc cggacgacgg
     1561 cgtgccggtg gtcctggtcc acggcaacac gtccgcggcg cgcttctggg aagagaccat
     1621 gctggcgctg cccgcgccct atcgcgcgct ggcgcccgac ctgcgcggct acggccagac
     1681 cgaagcccta ccgctagacg ccacgcgcgg cctgcgcgac tgggccgacg acttgcacgc
     1741 gctggtcaag gcgctggcgc tgggtccggc ccactgggtc ggctggtcca tgggcggggg
     1801 cgtggtcatg cagtacgcca tcgaccaccc ggcggacgtg cgctcgctga ccctggtcga
     1861 tccgctcgcg ccctacggct tcggcggcac gaaggatacc gcgggcacgc cgacgtaccc
     1921 ggatttcgcc gggtcgggcg ggggcaccgc caacccggag ttcgtcaagc tgttgcgcga
     1981 gggcgaccgc agtgccgata gccccaccag cccgcgcaac gtgatgaaca ccttctactt
     2041 caagccgccg tttaaggccg cacccgaccg cgaagaggtg ttcgtcaccg agatgctctc
     2101 gatgcgcatc ggcgacgact tctatcccgg cgacatgacc acctccgaga actggcccgg
     2161 cgtggcgccc ggcccgcgcg gcatcaacaa cgcgatggcg cccggctact gtgaccggag
     2221 cggcttcgcc caggtcacgc cgcagccgcc ggtgctatgg gtac
//
LOCUS       DASUYC010000605         5834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1659479, whole genome shotgun sequence.
ACCESSION   DASUYC010000605 DASUYC010000000
VERSION     DASUYC010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5834
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1659479"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="VF276_09845"
     CDS             complement(<1..667)
                     /locus_tag="VF276_09845"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX6607203.1"
                     /translation="MKPLRGVILDVDGTLVDSNEAHIAAWVDALRQSGYDVSPDTIRP
                     LIGMGSDTLLPAAVGIEKATPEGQRLSKLHSRIFESEYLPRVEAFPCTHELLQHMRAR
                     GLRLVLASAAKGHIMDTLLDLAGAECLIEDDPPPRAVHSSKPDPEIVQAALDRLGYTR
                     DEVVMIGDTPYDIAAAAQLGIPVIAVRCGGYADADLAGALALYDDPADLLAHYAESPL
                     GRRA"
     gene            complement(664..1863)
                     /locus_tag="VF276_09850"
     CDS             complement(664..1863)
                     /locus_tag="VF276_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607204.1"
                     /translation="MDSAEYLRDTCVRAERALARAWGGAVRLAPGQPLTARGNVCRFL
                     LRDGPPGAPPSVIVKRARIRPGQRYEPDAAEYAGTAWLLFNDWAGLQFLTEVGGPWAP
                     APRFYAGDREAGLIVLEDLGPAATLDDLLCGEDPARAEAALLALAATLGRMHAATVGR
                     QAEFDRIRAALGPGANGSSGAGYEWLAAVLQHTAAALGLRLPPGVDAELRALIAAIRD
                     PGPFLAYTHGDSCPGNCLCTGATVRLCDFELGAFRPALLDGICGRIHFPTCRSVTRVP
                     PDIPPRMEAAYRAALAGGCPAAADDGYFYRAVVEACIYWALLRCQWSMPAAVLPDSAS
                     EWGVPALQQIVLIRFDLAAQVTAEFGHLEAIGATFAAMARELRTRWPPEADAAPYHAA
                     FRSGVAV"
     gene            2296..3588
                     /locus_tag="VF276_09855"
     CDS             2296..3588
                     /locus_tag="VF276_09855"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6607205.1"
                     /translation="MYGDTERALNPRIIMYSQDGFGLGHMRRTNSIAGWFLRTCPEAC
                     ILTLSDSRLGEFFASSRNHDYLKLPSIVKTGPGNWRAVNLALPFDEIYRLRQEVIRSA
                     VLNFRPHILLVDHMPHGAMGELLPTLEALKSSGADTKIVLGLRDILDAPAVVQDRWRV
                     ERAFEAIERYYDMVLVYGQRDVFDLAKTYRFPRAVRNRLRYCGYVCTPHMSQAAAGIR
                     ATHLAGAPEGTPLIVSMAGGGDDAYPMMRSLIDALPAILARQACRVVLIVGPFMPPES
                     QRELQERARDLPVSIIRSVDDTPGYIEAADLVVAMAGYNTTVEILRGGGRAILIPRPG
                     PSAEQRMRSRLFAERGWVATIDPDDVSVDTVAAAVVQSLQQEPRSGATIPPDLDGLRT
                     AVDLLRGLLPAPPRESLLPLPLIAAEHKDQALGSPVPL"
     gene            3566..4807
                     /locus_tag="VF276_09860"
     CDS             3566..4807
                     /locus_tag="VF276_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607206.1"
                     /translation="MDHPFRFDPQLPDLPVAFDPGAVTRLFAAYEPGGGLLRAHRLQD
                     VKDQPGARCVATWELLVDQGEGAARPTIGVVEVTPAGPAARLFTDDPALPWLAPAMDP
                     AGMRARFATLPGPAVTGCTITPVRYKPGARCVFRYDLQTADGPRAFFGKLLAQDGDRL
                     MDTIGALHDLSRRVPALPRIPAPVAYWPEVHLLVQPAVGSGTELNTVAFDPAEDVAQR
                     DAWLRDAGARLAALQTHVTVAGPLRTLAGDLAELHEYEAPMAQADPALATRYRDVLGE
                     LASVPAGQAEAVAVAAHGAFRTDQFMIQDGDLVMIDLDSFCWAGPARDPGNFLAYLRW
                     KAIRRPEQAAFIDHAGQMFLAGYRAAGAAPDPARLALYEAASLLKIAGRRFRSLTVKE
                     WSLVPQLLDAARAVLDQPVSL"
     gene            complement(4896..5708)
                     /locus_tag="VF276_09865"
     CDS             complement(4896..5708)
                     /locus_tag="VF276_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607207.1"
                     /translation="MKRVFQAVLVLAGALLLWPALVHAAPPAPQGKLVYTDDFDDGGK
                     KSGLEINLKGDPDFSRGFHPPGVYQMQLAKPNDTRAVLLPNQSYGELSAQVEIWDNSD
                     VLIGDASHGLVFRAQDPTHYYAALVDSRKQQFAIRKLDGGTWSDLLAWKPVEGIKQKS
                     EVNTLRVDASGATFTAYINGEQVGTAQDSAYTKGQIGLVVSNVDAQKPHYHFNNLRIY
                     STEPAPVLSPSTLPTSGQGAPGAPFAVLILGLALAGLGLALRRPRGAPARAE"
BASE COUNT          864 a         2035 c         2006 g          929 t
ORIGIN      
        1 aggcccgccg ccccagcggc gactcggcgt agtgggccag caggtcggcg gggtcgtcgt
       61 agagggcgag ggcgcccgcg agatcggcgt ccgcgtagcc gccgcagcgc accgcgatga
      121 ccgggatgcc gagttgggct gccgccgcga tgtcgtaggg cgtgtcgccg atcatcacca
      181 cctcgtcgcg ggtatagccc agccggtcca gcgccgcctg cacgatctcc gggtcgggct
      241 tcgagctatg caccgcgcgg ggcggcgggt cgtcctcgat caggcactcg gcgcccgcca
      301 ggtcgagcaa ggtgtccatg atgtgcccct tggccgcgct cgccagcacc aggcgcaggc
      361 cgcgggcgcg catgtgctgc aagagttcgt gcgtgcaggg gaacgcctcc acccgcggca
      421 gatactcgga ctcgaagatc cggctgtgca gcttgctgag gcgttgtccc tccggggtgg
      481 ctttctcgat gcccacggcg gcgggcagca gcgtatcgct gcccatgccg atcaggggcc
      541 ggatcgtgtc cggcgacacg tcgtagccgc tctggcggag cgcatcgacc caggcggcga
      601 tgtgggcctc gttgctgtcc accagcgtgc cgtctacgtc tagaatgacc ccgcgcaacg
      661 gtttcatacg gcgactcctg accgaaaagc ggcatgatag ggggcggcat cggcttccgg
      721 cggccagcgg gtacgcaatt cccgcgccat ggccgcaaag gtggccccga tagcctccag
      781 gtgcccgaac tccgccgtca cctgggcggc caggtcgaag cggatcagca caatctgttg
      841 caacgccggc acgccccatt cgctggcgct gtccggcagc accgccgccg gcatactcca
      901 ttgacaacgc agcagcgccc agtaaatgca cgcctcgacc acggcgcgat agaaatagcc
      961 gtcgtcggcg gcggcgggac agcctccggc cagggcggcc cgatacgcgg cctccatgcg
     1021 cggcgggatg tcgggcggca cccgcgtcac cgagcggcag gtgggaaagt ggatgcggcc
     1081 acagatgccg tcgagcaggg ccgggcggaa ggcgccgagc tcgaagtcgc acaaccgtac
     1141 cgtcgcgccg gtgcataagc agttgccggg gcaggaatcg ccgtgcgtgt aggccaggaa
     1201 cgggcccgga tcgcggatgg cggcgatgag cgcgcgcagt tcggcgtcca cgccgggcgg
     1261 gaggcggagg ccgagcgcgg cggcggtgtg ctgcaatacc gcggccagcc actcatagcc
     1321 ggcgcccgag ctgccgttcg cgcccgggcc gagcgcggcg cggatgcggt cgaactcggc
     1381 ctgccggccc acggtcgcgg catgcatccg gcccagcgtc gcggccagcg caagcagcgc
     1441 cgcttcggcg cgcgccgggt cctcgccgca cagcaggtcg tccagggtcg cggcggggcc
     1501 caggtcctcc agcacgatga ggccggcctc gcggtccccg gcgtagaagc gcggcgcggg
     1561 cgcccagggg ccgcccacct cggtcagaaa ctgcagcccg gcccagtcgt tgaacagcag
     1621 ccaggccgtg cccgcgtact ccgccgcgtc cggctcgtac cgttggccgg gccggatgcg
     1681 cgcccgcttc acgatgacgc tcggcggcgc gcccggcggg ccgtcgcgta gcagaaagcg
     1741 gcagacgttg ccgcgcgcgg tgagcggctg ccccggcgcg aggcgcacgg ccccgcccca
     1801 ggcacgggcc agcgcgcgct cggcgcggac gcaagtgtcc cggagatact cggcgctatc
     1861 catggcgatc ctcctgggct gctggaagat ccgatgctgg agtatagggg ttggccggtc
     1921 ccattcaata ttaccggatc gcgcggcaaa ccgaaaggtt ccgtttcccc cctcattggg
     1981 cggcaaaccg aaaggttccg tttccccttc ccgcggcggg aaggggggcg ggggggttag
     2041 gttggtccct ccacccccca atttgccgcg ggcgggtaac cctcgcaggg gaatcgcaat
     2101 tatattagcg attgaatttt cagttagaaa ataactgcct gtagtttacc actcgaatca
     2161 cccacgtaac agcggaatct tgctgcccat tgtattccgc tccccgatgg aaatctgccg
     2221 ccgcaggttg gtaacgatgg cgcttgcgcc atggtcccag cagtgaatca cccaactggt
     2281 gaggagggat ccccgatgta tggcgatacc gagcgcgcgc tgaacccgcg cattatcatg
     2341 tactcgcagg acggttttgg tctcggccac atgcgccgca ccaactcgat tgccggctgg
     2401 ttcctgcgaa cctgtcccga agcgtgcatc ctcacgctgt ccgattcccg gctcggcgaa
     2461 ttcttcgcca gctcgcgcaa ccacgattac ctgaagctcc cctcgattgt gaagaccggg
     2521 ccgggcaact ggcgcgccgt caacctggcg ctgccgttcg acgagatcta tcgcctgcgc
     2581 caggaagtga tccgcagcgc ggtgctcaac ttccgccccc acattctgct cgtcgaccat
     2641 atgccgcatg gcgcgatggg cgaactgctg ccgaccctgg aggcgctcaa gagcagcggc
     2701 gcggacacca agatcgtgct ggggctgcgc gatatcctcg acgcgcccgc cgtggtccag
     2761 gatcggtggc gggtggagcg cgcctttgaa gcgattgagc gctactacga catggtgctg
     2821 gtctatggcc agcgggacgt gttcgacctg gcgaaaacct accggttccc gcgcgcggtg
     2881 cgcaaccggc tgcgctactg cggctacgtc tgcacaccgc acatgtcgca ggccgccgcc
     2941 ggcatccgcg ccacccacct ggccggcgcg ccggagggca ccccgctgat cgtgtcgatg
     3001 gccggcggcg gcgacgatgc gtatcccatg atgcgcagcc tgatcgacgc gttgccggcc
     3061 atcctggccc gccaggcctg ccgcgtggta ctcatcgtcg ggccgttcat gccgcccgag
     3121 tcccagcgcg agctacagga gcgcgcccgc gacctgccgg tgtcgatcat ccgctcggtc
     3181 gacgacaccc ccggctatat cgaagccgcc gacctggtgg tggcgatggc ggggtataat
     3241 acgacggtcg agatcttgcg ggggggtggc cgggcgatcc tgatcccgcg gcccggtccc
     3301 agcgccgagc agcgcatgcg gtcgcggctc ttcgccgagc gcggctgggt cgccacgatc
     3361 gatcccgacg acgtgtcggt ggacacggtg gcggcggcgg tggtccagag tttgcagcag
     3421 gaaccgcgta gcggggcgac tatcccgccc gatctggatg gtctgcgcac ggccgtggac
     3481 ctgttgcgcg ggctcttgcc ggccccgccg cgcgagagcc tgctacccct gccgctgatc
     3541 gcggcggagc acaaggacca agcccttgga tcacccgttc cgctttgatc cgcaactacc
     3601 cgatctcccg gtcgccttcg atcccggcgc cgtgacgcgg ctctttgccg cgtacgagcc
     3661 gggcgggggc ctgttgcgcg cgcaccggct gcaagacgtg aaagaccagc cgggcgcgcg
     3721 ctgtgtagcc acctgggagt tgctggtgga ccagggcgaa ggcgcggcgc ggcccaccat
     3781 cggcgtggtc gaggtcacgc ccgccgggcc ggcggcccgg ttgtttaccg acgatcccgc
     3841 gctgccctgg ctggccccgg cgatggaccc ggcgggcatg cgcgcccgct tcgccaccct
     3901 gcccggcccg gcggtcaccg gctgcaccat cactccggtc cgctacaagc cgggcgcgcg
     3961 ctgtgtgttt cgctatgact tgcagacggc ggacggcccg cgcgcgtttt tcggcaagct
     4021 gctggcgcag gacggcgacc ggctcatgga caccatcggc gccctgcacg acctgagccg
     4081 gcgcgtgccc gcgctgccgc gcattccggc gcccgtggcc tactggcccg aggtgcatct
     4141 gctggttcag cccgccgtcg gcagcggcac cgaactgaac acagtcgcct tcgaccccgc
     4201 cgaggacgtg gcgcaacgcg acgcctggtt gcgcgacgcg ggcgcccggc tggcggcctt
     4261 gcagacccat gtgacggtgg ccgggccgct ccgcaccctg gccggcgacc tggccgagct
     4321 ccacgagtac gaggcgccca tggcccaggc cgacccggcc ctggcgaccc ggtatcgcga
     4381 tgtgctcggc gaacttgcgt ccgtgccggc ggggcaggca gaagccgtcg cggtcgcggc
     4441 gcatggggcg ttccgcaccg atcagttcat gatccaggac ggcgatctgg tgatgatcga
     4501 cctggacagc ttctgctggg ccggcccggc ccgcgacccc ggcaacttcc tggcctacct
     4561 gcgctggaaa gcgatccgcc gtccggagca ggccgccttc atcgaccacg ccgggcagat
     4621 gttcctcgcc ggctaccggg cggcgggtgc cgcgccggac ccggcccgcc tcgcgctcta
     4681 cgaggccgcg tccttgctga agatcgccgg gcgccggttc cgcagtttga ccgtgaaaga
     4741 gtggtccctg gtgccccaac tgctcgacgc ggcgcgggcg gtcctcgatc aacccgtgtc
     4801 cctttagcgt tattggccgg ggcagcaggc cggtttcaaa ccggccccta catcagcgcg
     4861 aagcggcggg ccggttgaaa ccggtcccta ccgcgctatt cggcccgcgc cggggcgccg
     4921 cgcgggcgcc gcagcgccag ccccagcccc gccagcgcca gccccaggat cagcacggcg
     4981 aacggtgcac ccggcgcgcc ttgccccgac gtgggcaggg tggacgggga cagcacaggg
     5041 gccggctcgg tcgagtagat gcgcaggttg ttgaagtggt agtggggttt ctgggcgtcg
     5101 acgttgctca cgaccaagcc gatttgccct ttagtatagg cgctgtcctg ggccgtgccc
     5161 acttgctccc cgttgatata cgccgtgaac gtagcgccgc tggcgtccac gcgcagcgtg
     5221 ttaacctcgg acttttgctt gatcccctcg acgggtttcc aggccagcag atcgctccag
     5281 gtaccgccgt cgagtttgcg gatggcaaac tgttgtttgc gcgaatccac cagcgcggcg
     5341 tagtaatggg tcggatcttg cgcccggaag accagaccgt ggctcgcatc gccgatcagc
     5401 acgtcgctgt tgtcccagat ttccacctgg gccgatagct caccatagga ctggtttggg
     5461 aggagcacgg cgcgcgtgtc gttaggcttg gcgagctgca tctggtagac gccgggcggg
     5521 tggaagccgc gcgagaaatc gggatccccc tttagattga tctccaggcc gcttttcttg
     5581 cccccgtcgt cgaagtcatc ggtgtagacc agtttaccct gcggcgcggg cggcgccgcg
     5641 tggaccaggg ccggccatag cagtagagca ccggccaata ccaggacagc ctgaaaaacg
     5701 cgtttcatgg actttccctt tcactactca gaccagtacc gaccgcggtg cgggaccgcg
     5761 cccgcgtcac gcgcctcctt tcccccggtt tgctgcttgt cccccggacg ggcgccatat
     5821 ccgtcttacg gggt
//
LOCUS       DASUYC010000606         3361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_499853,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000606 DASUYC010000000
VERSION     DASUYC010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499853"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..1174)
                     /locus_tag="VF276_09870"
     CDS             complement(206..1174)
                     /locus_tag="VF276_09870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6607208.1"
                     /translation="MKGDMKTSLIIGSRNRPRLLLETVESVLRGTQVPDEIVIVDQSD
                     VPHPRLPTLTPDRPCAVRYLWSHTVGVSRAKNIALAAARYGVAIFTDDDILVDPEWLA
                     VVQGALAAAGPRAVITGRVLPSRAEVPGGFVPTLVVADTPAVYEGRINTDVLPGCHMA
                     AYRSALIGIGGFDERLGPGSPFRSAEDNDLGFRLLEAGYRIVFVPEAVVYHRAWRPAR
                     EYLPVRWAYGVAKGGFYTKYLSLADPYMLRRMVLDLARRTYAFPNRIQHERQRAIGDL
                     VYMAGVGYGAARWLLSQQGRRWPPKKHPFLNSGRGGSGRPARRRAP"
     gene            complement(1251..2165)
                     /locus_tag="VF276_09875"
     CDS             complement(1251..2165)
                     /locus_tag="VF276_09875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6607209.1"
                     /translation="MLQPVVDLTVAVATLDRPGGLVRCLDAVLSGCKLPAQVVVVDQS
                     WDRRAQDALARFQASRISIVYVRQRRRGLSASRNAAIARAWCPVIGMTDDDCVPDPGW
                     VAAIAKTFASTNPPDAMAGRILPLGPAVPGLYAISPRAGMQQTDFQGPREPWLVGSGG
                     NFAVRVDCLKRLGRYDERLGAGSPGQAAEDMDYIYRVLRAGGRIRYEPDALIYHERQT
                     RRKRLATRSSYGFGMGAFCGKWIRRRDLYAARILRRWLLWQGQDLLAGAGAGDWAGAY
                     EKYLSLQGTARGLVYGLRLPVTRTEALD"
     gene            complement(2150..3328)
                     /locus_tag="VF276_09880"
     CDS             complement(2150..3328)
                     /locus_tag="VF276_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607210.1"
                     /translation="MALAAAPPRAGSREWSHKRLAAIGLGLGLVTVTKLALLPCVLAV
                     GLFVTVVIAVPSWRAWPAVARQVAVVAVLFLIAVAPWWARNIVRYGNPVFPAALPVIG
                     RGVVISQLGRIDTEFVPGPPAWPLYPFLEPLDDRTGFGPLFILGLVPGFLGAAFRAPR
                     RPLILYLLVWLIMLPAWWVFTLHEPRFLLALFGLGMAFLPYALLTIPRNQRRYAWALV
                     AVTALFSVAVTADQALLPAARQPANRAAFYNRVWGVDPTVMSLPENEGLLYNTGYAPT
                     IPEYAAYYPLLGPGRTRLVLPIDIDGTTAGIVDRMRRAGIRYAYVAAAPQARAEVQRI
                     YPASLFDVVHISVVEAGEKSGARRYLYRPVPAADQDRGTWRYLFRLKDSVAVKQCCSQ
                     "
BASE COUNT          645 a         1188 c         1029 g          499 t
ORIGIN      
        1 ggggtcagcg taggcaagcg ggggtgcggc acgtcgctct ggtcgacaat cacaatttcg
       61 tcgggcacct gcgtgccccg cagcaccgac tccaccgtct ccagcagcag ccgcggccgg
      121 ttccgcgagg cgatgatcac ggaaatggtt ggcacatcca tctgcgcccc cggctccccg
      181 gcctgtgcag cccgactcat cgccgtcaag gtgccctcct gcgagcgggt cgccccgatc
      241 ctccccggcc gctgtttaag aaaggatgct tttttggcgg ccaccttctg ccctgctgcg
      301 acagcagcca gcgcgccgcg ccataaccca cgccggccat atataccaga tctccaatcg
      361 cccgctgccg ctcgtgctgg atccggttcg ggaaggcata tgtgcggcgc gcgagatcca
      421 agaccatacg gcgcagcatg tagggatcgg ccagactgag gtacttggta tagaagccgc
      481 cttttgctac gccataggcc cagcgcaccg gcagatactc gcgcgccggg cgccacgccc
      541 gatgatagac gacggcttcg ggcacaaaca cgatgcgata tccggcctcc agcagccgga
      601 agcccagatc attgtcctct gctgaacgaa acggcgagcc tggccccaac cgctcatcga
      661 acccgccaat gccgatcagc gccgatcggt aggccgccat atgacaaccg ggcaacacat
      721 cggtattgat gcgtccttca tatacggccg gcgtgtcggc gaccaccagc gtcggcacaa
      781 acccgccggg aacttcggcg cgggagggca atacccggcc tgtgatcacg gcgcgcggcc
      841 cggccgccgc cagcgccccc tggaccaccg ccagccactc cggatccacc agtatatcgt
      901 catcggtgaa gatcgccact ccgtaccggg cggccgccag cgcgatgttc tttgcccggc
      961 tcacgcccac ggtgtgggac cacagatagc ggacggcgca gggccgatcg ggggtcagcg
     1021 taggcaagcg ggggtgcggc acgtcgctct ggtcgacaat cacaatttcg tcgggcacct
     1081 gcgtgccccg cagcaccgac tccaccgtct ccagcagcag ccgcggccgg ttccgcgaac
     1141 ctatgatgag agatgttttc atgtcacctt tcaacccacc ctggcgagtc ggggttctcg
     1201 gtgcccgcac tgcccgcgat gggcgcgcgg cgccgccggg caaccggcct tcaatccagt
     1261 gcctctgtgc gcgtaaccgg taggcgaaga ccatacacca gtccccgcgc cgtaccctgc
     1321 aaactcagat acttctcata agcgcccgcc caatcgcccg cgccggcacc ggccagcaga
     1381 tcctgcccct gccaaaggag ccagcggcgc aggatgcggg cggcgtatag atcgcggcgg
     1441 cgtatccatt tgccgcagaa agcccccata ccgaaaccat aactcgaacg cgtggccagc
     1501 cgctttcgcc gggtctgccg ctcatgatag atgagcgcgt ccggttcgta gcggatgcgg
     1561 cctccggcgc gcagcacgcg atagatatag tccatgtctt cggccgcttg ccccggagag
     1621 cccgcgccca gccgctcatc atagcgaccc aggcgcttga gacaatcgac ccgcaccgcg
     1681 aaattgcccc cgctgcccac gagccagggt tcgcgcggtc cctggaaatc cgtttgctgc
     1741 atgccggccc gcggagaaat tgcgtacagc ccaggcacgg cggggcccag cggcaaaatc
     1801 cttcccgcca tcgcgtccgg tgggttcgtg gaagcaaacg tcttcgcgat agcggccacc
     1861 cagccggggt cgggcacaca gtcatcatcc gtcatgccga tcaccgggca ccacgcccga
     1921 gcgatagccg cattccgcga cgcggacaac ccccggcgcc gctggcgaac atagacgatt
     1981 gaaatgcggc tcgcctggaa ccgggcgagg gcgtcctggg cccgccggtc ccaactttga
     2041 tccaccacca ccacctgggc cggcagtttg caccccgaga gcacagcatc caagcagcgc
     2101 accaggccgc cggggcgatc cagggtggcg acagcgacgg tcagatcgac tactggctgc
     2161 agcattgctt caccgccacg ctatccttca gccgaaacag atagcgccat gtgccgcggt
     2221 cctgatccgc tgcgggtaca ggccgataca ggtaacgccg cgccccgctt ttctcgccgg
     2281 cttccaccac cgagatatgc accacatcga acaacgacgc cggataaata cgctgcacct
     2341 cggcgcgtgc ttgcggcgct gccgcgacat aggcataccg gatccccgcg cgccgcatgc
     2401 gatccacaat ccctgccgtc gtaccatcga tgtcgatggg gagtacgagt cgggtgcgcc
     2461 cagggcccag gaggggataa tatgcggcat actcggggat cgtcggcgcg tatcctgtat
     2521 tgtagagcaa accttcgttc tcgggtaacg acattacagt gggatcgact ccccacacgc
     2581 ggttgtaaaa cgcggcccga ttcgccggtt ggcgcgcggc gggcaagaga gcctggtccg
     2641 ccgtcaccgc taccgagaac agtgcggtaa cggcaacgag cgcccaggcg taccggcgct
     2701 ggtttcgcgg tatcgttagg agcgcatagg gtaagaaagc catgcccagt ccaaacagag
     2761 ccagcaggaa tcgcggttca tggagcgtga agacccacca ggcgggcagc attatcagcc
     2821 acaccagcag gtacaggatc agcggccggc gtggcgcccg gaaggcggca ccgaggaacc
     2881 ccggtaccag cccgagaatg aacaacggcc cgaatcccgt tctgtcgtcc agcggctcca
     2941 ggaacgggta gaggggccac gcgggcggcc ccggcacgaa ctccgtatcg atccggccca
     3001 actggctgat gaccaccccc cggccgatca cgggcagcgc cgccgggaag acggggttcc
     3061 cgtagcggac gatgttacgc gcccaccagg gcgccacggc gatcaggaat aacacggcca
     3121 ctaccgccac ctgccttgcc acggcgggcc aggcgcgcca ggacgggaca gcaataacca
     3181 ccgtcacgaa taggccaacg gccagtacgc aaggcaggag cgccagcttc gtcaccgtga
     3241 cgaggcccag ccccaggccg atcgcggcca gccgtttgtg gctccattcg cgtgatcccg
     3301 ctcggggggg cgccgcggcc agcgccatcg ttgtcatcaa cagaccggct gcgacaacgt
     3361 c
//
LOCUS       DASUYC010000607         3200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_253652,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000607 DASUYC010000000
VERSION     DASUYC010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253652"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="VF276_09885"
     CDS             <1..620
                     /locus_tag="VF276_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX6607211.1"
                     /translation="RELEAVESITVRHLVASVRLSAGVELRRAHRIRPAISTLRDVPI
                     QDRTATFDLGDLEPDRPPALLLELIVPTRAAGAFRIAAVTLAYDVPGPQGSTSGAQAR
                     ADAVLEYSTSGMGPLDPAVMNTVEKVNAFSLQTRALDEAAAGNIQGATTKLRSAATRL
                     LDMGETDLAQAAEQEAQKLEQSGQVSAEGAKKLHYATRRLTQRLE"
     gene            658..2673
                     /locus_tag="VF276_09890"
     CDS             658..2673
                     /locus_tag="VF276_09890"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX6607212.1"
                     /translation="MLTCEECGKLHPAGTTTCDVCGSTLTAWPYVANSDLGQELGLDR
                     LPPNSELGRVLAAPLRELTGALCAFCQSTNRPGATFCANCGHALIAPPADPATPAEGI
                     GQLPPGAFLQDRYRILGRVAQGGMGAVYEALDEQHDGARVAVKEMTPTSVAPEDRTQA
                     LKDFMREATILATLDHPNLPHFYGVFTEEGKYYLVMEFIEGQTLEQVVSGTTAFLPES
                     RVLIWAAQLCDVLAYLHRQDPPIIYRDTKPGNIILLDSEDHVKLIDFGIARFHRQGRS
                     TDTAAFGTAGYAPPEQYGNGQTDQRSDVYALAATLHHLLSGHDPVQNPFHWRPIRSYN
                     PHVSRQVDQAIMRAVELKPELRYPGIDEFARALGLRPVLVPAAEPATAALPREAAANL
                     PAPSSMPTTPVGPGDLPPTEEAERRPVRPRRRPPREVPVPVPATPPGLVPAPALAGTT
                     AVAAAPAEGPQLSISETVLDLGTAGRKARKGRMVNLVNAGQGQLKGIVHVTQPWLAVS
                     QVQFQGNVGALSVGAKRDGLQLGRAVLRVPNVFAWAWASGGRMVGRWVFLALIALMIW
                     GGTQLGMRPTDALLNGVALLVGTLAAAQVWLWLVARHVRWFVPAPRMNEGAVIIDSNG
                     GQRRLEVRVLARPSWLRRALAWFVLTVLLIGELVAAILGLLALLSGT"
     gene            2831..>3200
                     /locus_tag="VF276_09895"
     CDS             2831..>3200
                     /locus_tag="VF276_09895"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX6607213.1"
                     /translation="MAIRCQNCGNMNPDDYAFCDECGARLTADTSSAAAAPMGGEPAA
                     TPVAATAGAGTMTGGTVRCTNCGAENVAGAAFCDECGAALPQPTVEDTTVTSPPPPAY
                     GAPAAPYMPVTDTTTTTTEAT"
BASE COUNT          488 a         1165 c         1091 g          456 t
ORIGIN      
        1 cccgcgagct cgaagcggtc gagtcgatca ccgtgcgcca cctggtcgcc agcgtgcggc
       61 tcagcgcggg cgtggaactg cgccgcgccc accgcatccg cccggctatc agcaccctgc
      121 gcgatgtgcc catccaggac cgcaccgcca ccttcgacct gggcgacctg gagccggacc
      181 gcccgcccgc gctgctgctg gagttgatcg tgcccacgcg ggcggcgggc gccttccgca
      241 tcgccgcggt cacgctggcc tacgatgtgc ccggcccgca gggcagcacc agcggcgcgc
      301 aggcccgcgc cgacgcggtg ctcgaataca gcacgtcggg catggggccg ctcgacccgg
      361 cggtgatgaa cacggtggag aaggtcaacg ccttcagctt gcagacgcgt gcccttgacg
      421 aggccgccgc gggcaacatc cagggcgcca ccaccaagct gcgctcggcg gccacccgcc
      481 tgctcgacat gggcgagacc gacctggccc aggccgccga gcaggaagcg caaaaactgg
      541 agcagagcgg ccaggtgtcc gccgaagggg ccaagaagct gcactacgcc acccgccgct
      601 tgacccagcg gctggaatag tagtaacgtc cggcgccgct tgtgcgtaga tcatagtatg
      661 ctgacttgtg aagagtgcgg caaactgcac ccggcgggta ccactacctg cgatgtgtgc
      721 ggcagcacgc tgaccgcctg gccctacgtc gccaatagcg acctgggcca ggagttgggc
      781 ctggaccgcc tgccgccgaa cagcgaactg ggccgggtgc tggccgcgcc gctgcgcgaa
      841 ctgaccggag cgctctgtgc cttttgccag agcacgaacc gccccggcgc caccttctgc
      901 gcgaattgcg gccacgccct catcgccccg cccgcggacc cggcgacccc cgccgagggt
      961 atcggccagc taccccccgg cgccttcttg caggatcgct accgcattct cggacgcgtg
     1021 gcccagggcg gcatgggcgc cgtctatgag gcgctggacg agcagcacga cggcgcgcgc
     1081 gtggccgtca aggagatgac gcccaccagc gtcgcccccg aagaccgcac ccaggcgctg
     1141 aaggacttca tgcgcgaagc gaccattctg gcgacgctcg accaccccaa cctgccgcac
     1201 ttctatggcg tcttcaccga agagggcaaa tattacctgg tcatggagtt catcgagggc
     1261 cagacgctcg aacaggtggt ctccggcacc acggccttcc tgcccgaatc gcgcgtgctc
     1321 atctgggccg cgcaactctg cgacgtgctg gcctatctgc accgccagga cccgccgatc
     1381 atctaccgcg acaccaagcc gggcaacatc atcctgctcg acagcgagga tcacgtcaag
     1441 ctgatcgact tcggcatcgc gcgcttccat cgccagggcc gcagcaccga caccgccgcc
     1501 ttcggcacgg cgggctatgc cccgcccgaa cagtacggca acggccagac cgaccagcgc
     1561 tcggatgtct acgccctggc cgctacactg caccacctgc tcagcggcca cgacccggtg
     1621 cagaacccct tccactggcg gcccattcgc tcgtacaacc cgcacgtctc ccgccaggtg
     1681 gaccaggcga tcatgcgcgc ggtggaactc aagcccgaac tgcgctaccc cggcatcgac
     1741 gagttcgcgc gggcgctggg gctgcgcccg gtgctggtgc ccgcggcgga accggccacg
     1801 gcggcgctgc cacgcgaagc cgctgccaac ctgcccgcgc cttcttccat gccgaccacg
     1861 cctgtcggtc ccggcgacct gccgcccacc gaggaagccg agcgccgccc ggtgcgtccg
     1921 cgccgccgcc cgccccgcga ggtgccggtg ccggtgcccg ccacaccgcc cggcctggtg
     1981 cccgcgcccg ccttggccgg gacgaccgcc gtggcggcgg cccccgccga gggtccgcag
     2041 ctttctattt ccgagacggt gctcgacctg ggcaccgccg ggcgcaaggc gcgcaagggc
     2101 cggatggtaa acctggtcaa cgccgggcag ggccagttga agggcatcgt ccatgtcacg
     2161 cagccctggc tggccgtcag ccaggtgcag ttccagggca acgtgggcgc gctctcggtc
     2221 ggcgcgaagc gcgacggcct gcaactgggc cgtgccgtgt tgcgggtgcc caacgtgttc
     2281 gcctgggcct gggccagcgg cgggcgcatg gtgggccgct gggtgttcct ggccctcatc
     2341 gcgctgatga tctggggcgg cacccagctt gggatgcgcc ccaccgacgc cctcttgaac
     2401 ggcgtggccc tgctcgtcgg gacgctggcc gcggcccagg tctggctgtg gttggtggca
     2461 cgccacgtgc ggtggtttgt gccggctccg cgcatgaacg agggagcggt gattatcgac
     2521 agtaacgggg gccagcggcg gctggaggtc cgcgttctag cgcggccctc ctggctgcgg
     2581 cgcgcgctgg cctggttcgt tttgacggtg ttgctcatcg gcgaactggt ggcggccatc
     2641 ctgggcctgt tggcgttgct gagcggcacg tgaaccgtgg atccccgcgt ggcgacgggt
     2701 cgcgcgagcg ccgcgccggc agcgggtagg tttccgtgtt tgcagcgccg gtgcggtcgg
     2761 atataatgag gcaacctggt gaggtggtcc cttcgacggc gatctttgag tgcgtattaa
     2821 ggaggagacg atggcaattc ggtgtcagaa ctgcgggaac atgaacccgg atgactatgc
     2881 cttttgtgac gagtgcggcg cacgcctgac ggcggatacg agcagcgccg ccgcggcccc
     2941 gatgggcggc gagccggccg ccaccccggt ggccgcaacg gcgggcgcgg ggaccatgac
     3001 cggcggcacg gtgcgctgca ccaactgcgg tgccgagaat gtcgcgggcg cggctttctg
     3061 cgacgaatgc ggcgcggcgc tgccccagcc caccgtcgaa gacacaacgg tcacgagccc
     3121 gccgcccccg gcctacggcg ccccggcggc cccctacatg ccggtgaccg acacaactac
     3181 gaccacgacc gaggcgacga
//
LOCUS       DASUYC010000608         2934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1424202, whole genome shotgun sequence.
ACCESSION   DASUYC010000608 DASUYC010000000
VERSION     DASUYC010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1424202"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="VF276_09900"
     CDS             complement(<1..900)
                     /locus_tag="VF276_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607214.1"
                     /translation="MNRLRSTGAPAVSQFQLIGGIRHEAGDCIVSAEPGGATQVRKGA
                     LYVVTEPAGDPALGGEACALAQATVAHEYYADPSPSVTTSLTNALNKANGTLIQYNRK
                     VLATSDPPAGLPRKIRVGLSAAIVRPGQMYLCQLKPGLILWTHQGAVSAFPRPAQWAQ
                     SAATVNGDGEIVGNFYPAAPLGTGPLVEADYAFRRFDSGDLLILCSSNLAGVLDEDAL
                     AGALPGRPAADVVEYLYNLARDAGLAEAHAMAVEMSAAPVSRRSTQPITLPPPPAWAP
                     EATPDPPHPATAPPPAPAGPDVPV"
     gene            complement(1045..1275)
                     /locus_tag="VF276_09905"
     CDS             complement(1045..1275)
                     /locus_tag="VF276_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607215.1"
                     /translation="MQKWDYLFLTARYEGVWHPWLVDDVAVPNGNRITLAGFMNDLGQ
                     QGWELVGTTNVTAHQYNLFFKRPCNKGLDPRD"
     gene            1759..>2934
                     /gene="eno"
                     /locus_tag="VF276_09910"
     CDS             1759..>2934
                     /gene="eno"
                     /locus_tag="VF276_09910"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007503205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX6607216.1"
                     /translation="MITRAPIREVVAREVLDSRGNPTVEVELTLENGVQGRTMVPSGA
                     STGAHEAVELRDGDKRRYGGKGVLQAVKHVEDDIKPKVLGFNALDQLGLDALLLELDG
                     TPNKGKLGANAILGVSMAAAHAAAASLGLPLYRYLGGVSARTLPVPMFNILNGGKHAA
                     NSTDFQEFMVMPVGAPSFREGLRWGVEVYQALKKVLAGRGYNTGIGDEGGYAPSLKSN
                     EEAIEVILAAIEQAGYRPGEDLFIALDPAITELYSDGKYQLPKEGRSLSTDEMIDFWA
                     GWIAKYPIISLEDGLAEDDWAGWTKLVARVGDKVQLVGDDFLVTNPERVRRAIQERAA
                     NSLLTKVNQIGTMSEAIEAVETAQHAGWTAVISHRSGETDDATIADLAVALNCGQIKT
                     "
BASE COUNT          482 a          925 c         1065 g          462 t
ORIGIN      
        1 cacgggcaca tccggccccg cgggggcggg gggcggggcc gtggccgggt gcggcggatc
       61 gggtgtggct tcgggcgccc aggcgggcgg cgggggcagc gtgatcggct gggtgctgcg
      121 gcgactgacc ggcgccgccg acatctcgac ggccatggcg tgggcctcgg ccaggccggc
      181 atcgcgggcc aggttataca ggtattcgac cacatcggcg gcggggcgcc ccggcagggc
      241 cccagccagc gcgtcttcgt ccagcacgcc ggccaggttg cttgaacaga ggatcagcag
      301 atcgccggag tcgaagcggc ggaaggcata atcggcctcg accagcgggc cggtgccgag
      361 cggagcagcc gggtagaaat tgccgacgat ctcgccgtcg ccgttcacgg tggccgcgct
      421 ctgtgcccac tgcgccgggc gcgggaaggc cgagacggcg ccctggtggg tccagaggat
      481 gaggcccggc ttcaactggc acaaatacat ctggccaggg cggacgatgg ccgcgctcag
      541 gccgacgcgg attttgcgcg gcagcccggc gggcgggtcg ctcgtggcca gcaccttgcg
      601 gttgtattgg atcagtgtgc cgttggcctt gttgagcgcg ttggtcaggc tggtggtgac
      661 gctaggggag gggtcggcgt aatactcatg ggccacggtg gcctgggcca gggcgcaggc
      721 ctcgccgccg agcgccgggt cgcccgccgg ctcggtcacg acgtagagcg cgcctttgcg
      781 cacctgcgtg gcgccgcccg gttccgcgct gacgatgcaa tcgccggctt cgtggcggat
      841 gcctccgatt agctgaaact ggctcacggc cggagcgccg gtcgaccgca gtcggttcat
      901 ggtctctcct ggcgggcata ggcaacggga cgtgctttgc ccccattgta cgtcacctgc
      961 tgatatatgt caacgagtgt ggggtatgtt cctgggcgca gatatataga cgacccgctg
     1021 catggtccca ggcgccggat cggctcagtc gcgcgggtcc agccccttat tacaaggccg
     1081 cttgaagaag aggttgtact ggtgcgccgt cacattagtt gtgcccacca gttcccagcc
     1141 ctgctggccc aggtcgttca tgaacccggc gagggtgatg cggttgccgt tgggcacggc
     1201 cacatcgtcg acgagccagg gatgccagac cccctcatag cgggcagtca ggaataagta
     1261 gtcccacttc tgcatggcga tggacccctt tcgtagtcgc ggccctacgc taaggacaag
     1321 ccggccggtg cggatcgcta catcgcttct atccatcgcc gccagtataa cacatcagcc
     1381 tgccgggcac cgggcgcgcc gacccgcccc ccgcccctca aggggcgggc tagcattacg
     1441 aagcccgcct gcgcgggctg ggccagggga gacaggccgt gcaggcggcc tttgtaaagg
     1501 tagccggagg cttgagcctc cgggcgacgc cgcgcggcgc cgggttgcgt tgtccccggt
     1561 ctggcgggaa gggattgcaa caggggaccg ggtgggctgt gttatcatgg gaggggtatc
     1621 ccgcgggccg gcaccgcctg cccgcgtggc gcttgcaatg cgccggggga ttgcgtatag
     1681 tacaccgaaa acggcagggt atccccgccg gtgcgagact gccggcttgc tgcgccggcc
     1741 acaggaggtt ggatagatat gattacgcgt gctcccattc gcgaggtagt ggctcgcgag
     1801 gttctcgact cccgcggcaa cccgaccgtg gaggtcgaac tgaccctgga gaacggcgtg
     1861 caggggcgca ccatggtgcc gtcgggcgcg tccaccggcg cgcacgaagc cgtcgaactg
     1921 cgcgatggcg acaagcgccg ctacggcggc aagggcgtgt tgcaggcggt taagcatgtc
     1981 gaggacgaca tcaagccgaa ggtgctcggc ttcaacgcgc tcgatcagct tggtctcgac
     2041 gcgctgctgc tggaactgga cggcacgccg aacaagggca agctcggcgc caacgcgatc
     2101 ctgggcgtgt cgatggccgc tgcgcatgcc gcggcggctt cgctgggcct gccgctctac
     2161 cgctacctgg gcggggtgtc ggcgcgcacg ctgcccgtgc ccatgtttaa catcctcaac
     2221 ggcggcaagc acgccgcgaa ttcgaccgac ttccaggagt tcatggtcat gccggtgggc
     2281 gcgcccagct tccgcgaggg gctgcgctgg ggcgtcgaag tctaccaggc gctcaagaag
     2341 gtgctggcgg gccggggcta caacaccggc atcggagacg agggcggcta cgccccgtcg
     2401 ctgaagtcga acgaagaggc catcgaggtg atcctggccg ccatcgagca ggcaggctac
     2461 cggcccggcg aagacctgtt tatcgcgctc gacccggcca tcaccgaact ctacagcgac
     2521 ggtaagtacc agttgcccaa agagggccgc agcctcagca cggacgagat gatcgacttc
     2581 tgggcgggct ggatcgccaa gtacccgatt atctcactgg aagacggcct ggccgaagac
     2641 gactgggccg ggtggaccaa gctggttgcg cgcgtgggcg acaaggtgca actggtgggc
     2701 gacgatttcc tggtgacgaa ccccgagcgc gtgcgccggg cgatccagga gcgggccgcc
     2761 aacagcctgc tgaccaaggt aaaccagatt ggcaccatga gcgaggccat cgaggcggtc
     2821 gaaacggcgc aacacgccgg gtggacggcg gtgatctcgc accgctccgg cgagaccgac
     2881 gacgcgacga ttgccgacct ggctgtggcc ttgaactgcg ggcagatcaa gacg
//
LOCUS       DASUYC010000609         5685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1558421, whole genome shotgun sequence.
ACCESSION   DASUYC010000609 DASUYC010000000
VERSION     DASUYC010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5685
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1558421"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1350
                     /locus_tag="VF276_09915"
     CDS             <1..1350
                     /locus_tag="VF276_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607217.1"
                     /translation="PEQVPLFPRAQALATLQLHPPAGIQTLAGDYPFLVVAVSRDAPH
                     EATTIPFVIHIATAGELRLELNPQRIVARRGVTGVRITNDANGPRPAVVRPSDPDAAL
                     VFKFGPALARQLGAPVAGPPTTTTSTGARLLADPSLVEVATDWLPPDAQGSQGQLELT
                     LPAAGAVELPMVVSTRKPIWTGPETPLVFDVLVSPPGVEWKESEAVRARGELVYTPYL
                     APWSRLPLAARRIIGALVGLLILALLLFLLFRPTGSPRPDVVAAGLTATAVSTSNAGA
                     ASAAATLTAVAGGPAGAHTGPPRITAFDFTTAPDGSIQIAWSVADADAVLLNNEAVPA
                     TGSRPVNTGTDQSLTLVASNALGTVSQSKGVIVMRPPEIVTFTAEPAQIAPGQQVILS
                     WHTVLAEKVSINGQPVAAPDGSVSTAPATTTEYVLTAESVLGQVQRSITVNVIPPTP"
     gene            1372..4311
                     /locus_tag="VF276_09920"
     CDS             1372..4311
                     /locus_tag="VF276_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607218.1"
                     /translation="MSNRITLQLEKNLLDLVPGESAVVRLRLTNSGTVVDAFKIAVEG
                     LDPAWYTLSATEARLFPQQGTIFALEVHVPPGTGVAAGIYPCTIIATSQDDPNEQEFA
                     QLNLQVAPIVDMGLDLEPRRVVGRAGLYGITLQNPSNVPRTIVLRVTDPDEALDYALG
                     TPVDAPAVAPLVLGTPLAEGAGRVEAEFDLPPVDNLLIPLRVRARRPLWTGPEQTFAF
                     QVQATPPGVEWQPAEARQIGGELVYAPLLAPFARLPLAWRRLLAIALPLLVLALLLWL
                     LFRPQPAPSVAGNPQSPTAPPTVAAQVPGGGGGAGGGAGASGGASASPTIPAAPLIRS
                     FRLILPPPVAATPGTVPPTVRPDLVWDVTAARDTAVFQSARPADNLGLSTSRLVDYQL
                     VATGTNGLTSTSTLGVLFLRPASITTFTASVLTMTLGQPTTLAWQVDGAKSVALDGVP
                     LDPGQMTGGSRDVVLPQPGTHTFILTATNPAGTVSRSVTVVVLPGPTPTPGIPTATVT
                     ATETATPLVAPPTVTPLPPGVPSPTAPPSPTITPPPTATPVPTNTPVLPTGTPPPLPV
                     DTATSTITAVPTGTATTTPTITPPATSTTTPTDTITPTGTATPSVTPTGTVTPTRTPA
                     CQTQVYNSTDVPFTVRPNTTPVVSTLVVPPGALITSIDVVGLTVDTITPYFLSAQLVS
                     PSGTRVTLFAGPCRGSQWTANNTGFNLSDSSGVPIGQRCPPGQSTYHPLENLSSFVGE
                     APGGTWYLVLSTIETPATVSRWGLAIQSGGLCPTATSTPRVSPTPTPPPLAACETTSA
                     AGLPLYMTDYGVYTSTLTIARPGTIRSITMPNLNLANTRGVALTGMLLAPDPNVRGIL
                     FDWSCGSQARMNVTLDDTARRDINSALCPQAGGPTPVPGTVFSGAYAPGPNALGIFTA
                     AEAQGTWQLQLLLPPPIGMRGGPDQADAGPLSTLWPGRGLVQSRALQPALLAQLTAWS
                     LNICYVP"
     gene            complement(4379..5212)
                     /locus_tag="VF276_09925"
     CDS             complement(4379..5212)
                     /locus_tag="VF276_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6607219.1"
                     /translation="MAELESQTGFAEVNGARLYYEVAGTGHPLVLIHAGIADRRMWDN
                     QFALFARQYRVIRYDLRGLGQSAMPPGPYGLYQDLYGLLQYLGIAQAHVIGVSIGGMT
                     AVDFTLAHPEMVTALIPVAAGVSGSRPSEDSKALFEPIDAAYEARDLDRVLELEMDLW
                     VVGPGRARDAIDPALRQAVESMERPNLEREFAGEEGTFERLDPPAAGRLGEIQAPTLV
                     IVGDADTPNTVANAETLAAEIPGARKVVLPNVAHMVPMEAPNAFNRIVLDFLGSLPAG
                     R"
     gene            5486..5665
                     /locus_tag="VF276_09930"
     CDS             5486..5665
                     /locus_tag="VF276_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607220.1"
                     /translation="MDRIIANLHIQISRQPKQTALGGRTQDKELRAARQARAIQEYSR
                     RREQTHSEFLLQGGR"
BASE COUNT          907 a         2183 c         1696 g          899 t
ORIGIN      
        1 cccgaacagg tgccgctctt tccccgcgcc caggccctcg ccacactgca actgcacccg
       61 cccgccggta tccagactct ggcgggcgat tatccgttcc tcgtcgtggc cgtctcgcgc
      121 gacgcgcccc acgaggccac cacgattccg tttgtcatcc atatcgcgac cgcgggcgaa
      181 ttgcggctgg aactcaatcc gcagcggatc gtcgcgcgac gcggcgtcac gggtgtgcgc
      241 atcacgaacg acgccaacgg cccgcgcccg gccgtcgtgc gccccagcga tcccgatgcg
      301 gcgctggtct tcaagttcgg cccggccctg gcgcgccagt taggggcgcc cgtcgccggc
      361 ccgccaacga cgaccaccag caccggcgcg cgcttgctgg ccgacccgtc gctcgtggag
      421 gtcgccaccg attggctgcc gcccgatgcc cagggcagcc agggccagct tgaattgacc
      481 ctgcccgccg ccggcgccgt cgaactgccg atggtggtga gcacgcgcaa gccgatctgg
      541 accgggccgg agaccccgct ggttttcgat gtgctggttt ccccgcccgg cgtggaatgg
      601 aaggaaagcg aggccgtccg cgcccgtggc gaactcgtct acacgcccta tctggcgccc
      661 tggtcgcgcc tgccgctggc cgcgcgtcga atcattggcg cgctggtggg tctgctcatc
      721 ctggccctgc tgctcttctt gctcttccgg cccaccgggt ccccgaggcc cgatgtcgtc
      781 gccgcgggcc tgacggccac cgccgtcagc acgagcaacg cgggcgccgc gtctgccgct
      841 gccacgttga ccgctgtggc cggtgggccg gcaggcgccc acaccgggcc gccgcgcatc
      901 accgcgttcg acttcacgac cgcgccggac ggcagcatcc agatcgcctg gtcggtggcc
      961 gatgccgatg cagtgctgct caataacgaa gccgtcccgg ccaccggctc gcggcccgtc
     1021 aataccggca cggatcagtc gctgaccctg gtcgccagca acgcactggg tacggtcagc
     1081 cagagcaagg gcgtcattgt catgcgtcca ccggagatcg tcacgtttac cgccgaaccg
     1141 gcgcagatcg cccccggcca gcaggtcatc ctctcctggc ataccgtgct ggccgagaaa
     1201 gtgtcgatca acggccagcc cgtggccgcc cccgacggca gcgtcagcac cgcgcccgcc
     1261 acgaccaccg agtacgtgct gaccgccgaa agcgtcctgg gccaggtgca gcgcagcatc
     1321 accgtgaacg ttatcccgcc cactccctga gccgccggaa ggacaggtcg catgtcaaac
     1381 cgcatcacgc tgcaactcga aaagaacctg ctcgacctgg tgcccggcga aagcgcggtg
     1441 gtgcgcctgc gtctgaccaa cagcggcacc gtggtcgacg ctttcaagat cgcggtagag
     1501 ggcctcgatc ccgcctggta cacgctctcg gccaccgaag cccggctgtt tccgcagcaa
     1561 ggcaccatct tcgccctcga agtccacgtg ccgccgggca cgggcgttgc cgccgggatc
     1621 tacccctgta ccattatcgc cacctcgcag gatgacccca atgagcagga attcgcgcaa
     1681 ttaaatctgc aagtagcacc aatcgtcgat atgggcctgg atctggaacc ccgtcgggtg
     1741 gtcgggcggg cgggcctcta cggcatcacg ctgcaaaatc ccagcaacgt cccgcgcact
     1801 atcgtcttgc gcgtcaccga tcccgacgag gccttggact acgcgctggg caccccggtc
     1861 gatgcccccg cggtcgcgcc gctggtcctg ggcaccccgc tagcggaggg ggccgggcgc
     1921 gtggaggccg agttcgatct cccaccggtg gacaacctgc tgatcccgtt gcgggtgcgg
     1981 gcgcgacgcc ccctctggac cggcccggaa cagaccttcg ccttccaggt gcaggctacg
     2041 ccgcccggtg tcgaatggca acccgccgag gcccggcaaa tcggcggcga actggtctat
     2101 gcgccgctgc tggcgccgtt cgctcgcctg ccgctggcct ggcgccggtt gctggcgatc
     2161 gcgctgccct tgctggtgct ggcgctgctg ctctggctgc tgttccgtcc acaacccgcg
     2221 ccctctgtcg ccgggaatcc gcagtcgccc accgcgccgc ccaccgtggc cgcgcaggtg
     2281 ccgggtggcg gcggcggggc cggcggtggg gcaggggcgt ccggcggcgc gtccgcgagc
     2341 ccgaccatcc cggcggcgcc gctcattcgc tctttccggc tgatcttgcc cccgcccgtc
     2401 gccgccacac cgggcactgt gccgcccacc gtgcggcccg atcttgtctg ggatgtcacc
     2461 gccgcccgcg acaccgccgt cttccagagc gcgcgcccgg ccgataacct gggcctgtct
     2521 acctcgcgcc tggtcgatta ccagttggtt gccaccggca ccaacggcct gaccagcacc
     2581 agcactctgg gtgtgctctt cctgcgcccc gccagcatca ctaccttcac ggccagcgtg
     2641 ctcactatga ctctcggcca gcccacgaca ctggcctggc aggtcgacgg cgcaaagagc
     2701 gttgcgctcg acggcgtgcc cctcgatccc ggccagatga ctggcggcag ccgcgatgtg
     2761 gtgctgcccc agccgggcac gcacaccttc atcctgactg cgacgaaccc ggcgggcacc
     2821 gtttcccggt cggttaccgt ggtcgtcttg cccggcccca cgcccacgcc cggcataccc
     2881 acggcgacgg tcaccgccac cgaaacggcg acgccgctcg tcgcgccccc caccgtcacg
     2941 ccgctgccgc cgggcgtgcc gtcgccgacc gcgccgccct cgccgacgat cacgccgccg
     3001 ccgactgcga ctcccgtgcc gaccaatacg ccggtcctgc cgaccggcac cccaccgccg
     3061 ctgccggtcg ataccgcgac cagcacgatc acggctgtgc ccaccggcac ggccaccacg
     3121 accccgacga tcaccccgcc ggcgacaagc acgaccacgc cgaccgatac catcacgccg
     3181 accggcaccg ccaccccatc ggtgacgccg accggcacgg tcacgcccac cagaaccccg
     3241 gcctgccaga cccaggtcta caacagcacc gatgtgccct tcaccgtccg gcccaacacc
     3301 accccggtgg tctccaccct ggttgtgccg ccgggcgcgc tcatcaccag catcgacgtg
     3361 gtcggcctga ccgtggacac gatcacgccc tacttcctgt ccgcccagct tgtcagcccg
     3421 tcggggacac gggtcacgct gtttgccgga ccttgccggg gcagccaatg gacggccaac
     3481 aataccgggt tcaacctgtc ggacagcagc ggcgtgccga tcgggcagcg ctgcccgccc
     3541 ggccagtcca cgtaccaccc gctggaaaac ctctccagct tcgtcggtga ggcgcccggc
     3601 ggcacctggt acctggtgtt gagcaccatc gaaaccccgg ccaccgtgtc gcgctggggg
     3661 ttggcgatcc agagcggcgg tctctgcccg acggccacca gcaccccgcg cgtctcgccc
     3721 acgccgaccc cgccgccact ggccgcgtgc gaaaccacgt ccgccgccgg tttgccgctg
     3781 tacatgaccg actacggcgt ctacacttcc acgctgacca tcgcgcgacc cggcaccatc
     3841 cgcagcatca ccatgcccaa cctgaacctg gcgaacacgc gcggcgtcgc gctgactggt
     3901 atgctgctcg cgccggatcc caatgtccgc ggcatcctct tcgattggag ttgcggcagc
     3961 caggcccgca tgaacgtcac cctggacgac acggcacggc gcgacatcaa ttcggcgctc
     4021 tgcccgcagg caggcggccc cacgccagtg cccggcaccg tcttctccgg ggcctatgcg
     4081 ccagggccga acgccctggg catctttacc gccgccgaag cgcagggcac ctggcagttg
     4141 caactgctgt tgccgccgcc catcgggatg cgtggcggtc ccgatcaggc ggacgccggg
     4201 ccactgtcca cgctctggcc ggggcgcggc ctcgtacaga gccgggcgct ccagccggcg
     4261 ctgctggccc agctcaccgc gtggagcctc aatatctgct acgtccccta gatacagcag
     4321 gggcgccgtg cagcctgcca cacggcgccc ctgcttctct agtctcttcc ccgttcgctc
     4381 agcgtccggc cggcaggctg ccaagaaagt cgagcacgat ccggttgaag gcgttgggtg
     4441 cctccatcgg caccatatgc gccacgttcg gcagcaccac tttgcgcgcg ccggggatct
     4501 ccgccgccag cgtctcggcg ttcgcgaccg tattcggcgt gtccgcgtcc ccgacgatca
     4561 ccagcgtcgg cgcctgaatc tcgcccaacc gcccggctgc cgggggatcg agccgctcaa
     4621 acgtgccttc ttcacctgca aactcgcgtt ccaggttggg ccgctccatg ctctccaccg
     4681 cctgccgaag cgccgggtca atggcatcgc gcgcgcggcc cggccccacc acccagaggt
     4741 ccatctccag ttccagcacc cggtccaggt cccgcgcctc atacgccgcg tcgattggct
     4801 cgaacagcgc cttcgagtcc tccgatggcc ggctcccgct cactcccgcg gcgaccggga
     4861 tcagcgccgt gaccatttcc ggatgtgcca gtgtgaaatc gaccgccgtc atgcccccga
     4921 tcgacacgcc gatcacatgg gcctgcgcga tgccgaggta ctgcagcaac ccgtagaggt
     4981 cctggtagag cccgtacggc cccggtggca tcgccgactg gcccaacccg cgcagatcat
     5041 agcgaatcac gcgatattgc cgggcaaaca gcgcgaactg gttgtcccac atacggcgat
     5101 ccgcgatccc ggcgtggatg agcacaaggg gatggccggt cccggccact tcgtaataca
     5161 agcgtgcgcc gttcacctcg gcgaaccccg tctgcgactc aagctcagcc atgctacttc
     5221 tcctttggca ctaatgagcg aacggtcaat ccctctgatt atagcacact tgtactacta
     5281 ctcgggtaac tcgttgcgta cttgcagccc tagacaatga ttttaagccg atcattgatt
     5341 ttcttaagtt tcatataaat cggtctaaag tcgtagtccc aacctattga ctttttccca
     5401 gaagcatgtt aatatggacc catcggaaat aaagattgtt aagtccgaag ttaattttct
     5461 caaggtacca ataaggagga ctaccatgga ccgcatcatc gccaacctgc acatccagat
     5521 cagccgccag cccaagcaga ccgccctcgg tggccggacg caggacaagg aactccgcgc
     5581 ggcgcggcag gcacgcgcta tccaggaata cagccgccgg cgcgaacaaa cccacagcga
     5641 atttctgctg cagggcgggc gctaatcgtc cgccccgcag caaca
//
LOCUS       DASUYC010000610         3210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_219771,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000610 DASUYC010000000
VERSION     DASUYC010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219771"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..399)
                     /locus_tag="VF276_09935"
     CDS             complement(190..399)
                     /locus_tag="VF276_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607221.1"
                     /translation="MIGNMYTWAFPTERIEAVNAAHRAEAEALNRVAPATGATRQAPR
                     LGHMARLIRALLRSLRPAPRPRFQP"
     gene            complement(468..839)
                     /locus_tag="VF276_09940"
     CDS             complement(468..839)
                     /locus_tag="VF276_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607222.1"
                     /translation="MSTRSMTTPTRHTTSGVLPQPAVLQRWMQQRRFRQAQARAYVRF
                     AAAYPVWVESLFDAHFLSHAAAPALAQYAATAQRPTAGEIALLWAAQFIGVHYQPTQA
                     QFADAVAVAGAFLDYLAAELQ"
     gene            1255..1653
                     /locus_tag="VF276_09945"
     CDS             1255..1653
                     /locus_tag="VF276_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607223.1"
                     /translation="MSQAPSNRKKRSATTQNVKAAITVAAVAATVGGWAALSIADGAA
                     AANAAPVPQTTAPAPQTSAPGSDQNPFTQQPGRRGRRPFFGNGAPSTGQVPGDQTPSL
                     GGSSGDQAPSLGGSSGGQLPAPLGRSRSSR"
     gene            1675..2595
                     /locus_tag="VF276_09950"
     CDS             1675..2595
                     /locus_tag="VF276_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX6607224.1"
                     /translation="MEELRFRAMGCEMKAILDADGPRARRALAQVPGWFETWEAALSR
                     FRPDSELSRLNARAGQIVRVSGVLWEVLQAALAAARRTGGLVTPTGLAALEAAGYDRT
                     FAAVPAAGTAAAPATAAPLPAWQAVRCIAGLQAVQVPAGVRLDFGGIAKGWAADRAAR
                     RLAAHAPALVDAGGDIRVSGPRAGGDPWPIGAGDPRDPDRDLALLALRGGAVATSGRD
                     YRRWQRGGVWQHHILDPRTGHPATTDVLSASVIAPTAVAAESAAKAVLILGSRTGPAW
                     LDAQPGLAGLLVLDDGQVRLSRRLPEYFWS"
     gene            2599..>3210
                     /locus_tag="VF276_09955"
     CDS             2599..>3210
                     /locus_tag="VF276_09955"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEX6607225.1"
                     /translation="MYSPAPGAPVPPDPEPSVSPWQVAVLVGGAVGTALVLAVLPIWL
                     PALSTSLFGPDPRGYWYLTRASGLVAYALAWMSMIFGVLISNRLARMWPGGPTALALH
                     EHTSLLALAFTAFHALVLLLLQDQTIHYSLPDIVIPFAGATYRPIQVGLGQIALYAFA
                     VVTVTFYLRRQIGQRAWRVIHGFSYAIFVLALLHGILSGTDSTN"
BASE COUNT          424 a         1079 c         1208 g          499 t
ORIGIN      
        1 gcagtactca acggatcttg ctcaccaatg gtatcagtat agcgcgccac cgggcggcgc
       61 acatcaggag cgaggccgat tcgtactccc ccgcgggggc gaacccgcgc cggggtattc
      121 acccctggtg cctccatcgc caggcgtagg gcagaccgcc gaggctccgc cttgcgccgg
      181 aggaccgggt cacggctgga agcggggccg gggcgccggg cgcaggctgc gcagcaaggc
      241 gcggatcagg cgcgccatgt gccccaggcg gggcgcttgc cgggtggcgc cggttgcggg
      301 cgccacgcga ttcagggcct ccgcctcggc ccggtgggcg gcgttcactg cctcgatacg
      361 ctcggtgggg aaggcccagg tgtacatgtt gccgatcatg accgtgctcc ttgtctgggg
      421 cggggcgccc gtctcgcccg cgccggatac gcgaaggccc gccgcgctta ttgcaactcg
      481 gcggccagat agtccaggaa ggccccggcc acggcgacag cgtccgcgaa ctgggcctgt
      541 gtcggctgat agtgcacgcc gatgaactgg gcggcccaca gcagggcgat ctcgccggcg
      601 gtgggccgct gcgccgtcgc ggcatactgc gccagggcgg gcgcggcggc atggctgagg
      661 aaatgggcgt cgaacaggga ctccacccag accggatagg cggcggcgaa tcggacgtag
      721 gcgcgggcct gcgcctggcg gaagcgccgc tgctgcatcc agcgttgcag tactgccggt
      781 tgcggtaaaa cgccgctggt cgtgtgccgg gtgggggtag tcatggaccg cgtgctcatc
      841 agggaacctc ctttgctgct gttgcgccgg ctgatatttg ctgcgcctgg cgggtgcgag
      901 ttgcttgctt gatggtttca ctatacggcg gcggggccgg cggcacatcg tgggaattac
      961 ggatcacggt acgtattccg ggcatgggcg cggggcggcg cggtgttacg tagccatacg
     1021 taggctcggt agattcggtg ccggatggcg cataggagct gcgtcgttct tacgcgattc
     1081 tgtaggtttc ttttatcttt ccttaaggtg cgggcggtat aattacacgt gtgtacgccg
     1141 agtacaccac gccgcatgtc tttgcgcggt cccgcgtggc tatggctgcg gggacaacgg
     1201 cgtcccggcg gggcgcgcag gggcggcggg tagttgggaa aggactgaca gcttatgagc
     1261 caggcacctt ctaatcgcaa gaagcgctcg gccacaactc agaatgtgaa agcggcgatc
     1321 acggtggctg cggtggcggc caccgtcggc ggctgggccg ccctcagcat cgccgacggg
     1381 gccgcggcgg cgaacgccgc gccggtgccg cagacgaccg cgcccgcgcc gcagaccagc
     1441 gcgccgggca gcgaccagaa tccctttacc cagcagccgg gccgccgcgg gcggcgcccg
     1501 ttcttcggca acggcgcgcc ctcgacgggg caggtgccgg gcgaccagac gccgagtttg
     1561 ggcgggtcgt cgggcgacca ggcaccgagt ctgggcggct cgtcgggcgg gcaactgccg
     1621 gcgccgctag ggcgctcgcg ctcgtcgcgc taaggccgcg ggaagcagag accgatggaa
     1681 gaactacgtt tccgcgccat gggttgcgag atgaaggcga tactggatgc cgacgggccg
     1741 cgcgcccggc gcgcgctggc ccaggtgccc ggctggtttg agacctggga ggcggcgcta
     1801 agccggttcc ggcccgacag cgaattgagc cgcctgaacg cccgcgccgg gcagatcgtg
     1861 cgggtgagcg gggtgctgtg ggaggtgttg caggccgcgc tggcggcggc gcgccgcacc
     1921 ggcgggctgg tcacgccgac agggctggcc gcgctggagg cggcgggtta tgaccgcacc
     1981 ttcgccgcgg tgcccgctgc cgggacggca gccgcgcccg ccacggcggc accgctgccc
     2041 gcgtggcagg cggtgcgctg cattgccggc ctgcaggccg tccaggtgcc ggcgggggtg
     2101 cgcctcgact tcggcggcat cgccaagggc tgggccgccg accgggcggc gcggcggctg
     2161 gcggcgcacg cgcccgccct ggtggacgcg ggcggcgata tccgggtcag cggcccgcgg
     2221 gccggcggcg acccctggcc catcggcgcg ggcgacccgc gcgaccctga tcgcgacctg
     2281 gccctgctgg ccttgcgcgg gggcgccgtc gccacgtcgg gccgcgatta ccgccgctgg
     2341 cagcgcgggg gagtctggca gcaccatatc ctcgacccgc gcaccggcca cccggcgacg
     2401 accgacgtgc tgagcgcgag tgtgattgcg cccacggcgg tggccgccga aagcgccgcc
     2461 aaggcggtgc tgatcctggg cagccgaacc gggccggcct ggctcgacgc ccagccggga
     2521 ctggccgggc tgttagtgtt agatgatggg caggtccggc tgagccgccg gctgccggag
     2581 tatttctgga gttaaggtat gtattctccc gcgccggggg cgcccgtccc acccgaccct
     2641 gaaccttctg tatcgccctg gcaggtggcc gtgctggtgg gcggggccgt gggcacggcc
     2701 ctggtcctgg cggtgctgcc gatctggctg ccggccctga gcacctcgtt gttcggcccc
     2761 gaccccaggg gctactggta cctgacccgc gccagcggcc tggtggccta cgcgctggcc
     2821 tggatgtcga tgatcttcgg cgtgctgatc agcaaccggc tggcgcgcat gtggccgggc
     2881 ggccccaccg cgctggcgct gcacgagcat accagcctgc tggcgctggc cttcaccgcc
     2941 ttccacgcgc tggtcctgct gctgctccag gaccagacca ttcactactc gctgccggat
     3001 atcgtgatcc cattcgccgg ggccacctac cgcccgatcc aggtaggcct gggccagatc
     3061 gcgctctacg ccttcgcggt ggtgacggtc actttctacc tgcgccggca gatcggccag
     3121 cgggcctggc gggtgatcca cggcttcagc tatgcgatct tcgtgctggc cttgctgcac
     3181 ggcatcctca gcggcaccga cagcaccaat
//
LOCUS       DASUYC010000611         2000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_994949,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000611 DASUYC010000000
VERSION     DASUYC010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994949"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..388
                     /locus_tag="VF276_09960"
     CDS             227..388
                     /locus_tag="VF276_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607226.1"
                     /translation="MMSAWRWGIGFVLLLGGAIFLWWLMHGYLPWGLPCTVLLFAVVF
                     GGIVYFGRQ"
     gene            693..>2000
                     /locus_tag="VF276_09965"
     CDS             693..>2000
                     /locus_tag="VF276_09965"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX6607227.1"
                     /translation="MSVEELPVPAPAPERDPRARVAPPTNGHAGPAPAPSGAAPAPGA
                     PGRARVAPARPGTGNGRYPVAPARPLPPGTRRLPARPGYAAPGRGRRGAKPLFVLRHH
                     RPHYRRTLAPSARKGGYIRTILAGLLGSTLLFLLAGLIGAGGVAWAAWSYFTDGLPSV
                     TSLHPTEFQSTKIYDRHGGLLYEISDPTLGKRTYLGIEQLPPDLINATIAAEDASFRE
                     NAGVDLQSILRAVYINLSGQGSSGASTITMQLVRGAMLPEKDEPTFRRKVREAILANR
                     VSETYSKDKILEIYLNDIYYGSQAYGVSAASQVYYGVAAKDLDLAQAAMLAGLPQAPS
                     YFDPHTNYREAKIRQEYVLDQMVKSGYITQAEATKAAAEDVHPVPTVAEAGPSQAPHF
                     VNYVRALLDNMYGPDMVNRGGLRVFTTIDLGFQELARRTAAAQI"
BASE COUNT          315 a          710 c          665 g          310 t
ORIGIN      
        1 gtcagtggtc agtggtcagt attatgaaga attgtcactc tgagcgcagc gaagagtctc
       61 gtggggatac aggatgaggc ttcttcgctg cgcgccagtg acctgcgatt cgtagcggcg
      121 cgatttatcg cgcccgtccc cgcgccgcga tctacgggcc ggcgtgtgac agccaggccc
      181 gcgtacgctc accggaacag cacaacgaaa ggcggaacag acaaacatga tgtcggcatg
      241 gcggtggggg atcggctttg tcctgctgct cgggggcgcg atatttctct ggtggctgat
      301 gcacggctac ttgccgtggg ggctaccgtg tacggtgctg ctctttgccg tcgtgtttgg
      361 cgggatcgtc tacttcgggc ggcagtgacg gatgggcggg cgggggcagg caccctgggt
      421 gggcgtcagc gtgttgcatc ccggtcgcca accaacctct cccccggccc ctccccgccg
      481 cggggagggg agtcactttc cggccatccc ggccgttccc cccttcccgc cgagggaagg
      541 ggggcagggg ggttaggttg gttggcgacc gggatgccgc ccttcgcgtg caaaatcatg
      601 acgcgcaccg gggaagcccc gccggctggc gaaaatccga ataagtgtgc tatactagcg
      661 gtggcggcta gtctatagat tgtgcggatc tcatgagcgt cgaagaactt ccggtccccg
      721 cgcccgcgcc ggagcgcgat ccccgcgccc gcgtggcgcc tcccaccaac ggccatgccg
      781 gccccgcgcc tgcccctagc ggggcagctc ccgcgcccgg tgcgccgggg cgggcccgcg
      841 tggcccccgc ccggccaggg accggcaacg ggcgctatcc ggtggccccg gcgcgaccgc
      901 tgccgcccgg cacgcgccgc ctgcccgccc gccccggtta tgccgcgccg ggccgggggc
      961 gccggggcgc caagccgctg ttcgtgctgc gccaccaccg tccgcattac cggcgcaccc
     1021 tggcgccgtc cgcccgtaag ggcggttaca tccgcaccat cctggcgggc ctgctcggca
     1081 gcaccttgct gttcttgctg gccgggctga tcggggccgg gggcgtggcc tgggcggcct
     1141 ggtcctactt caccgacggg ctgccctccg tcaccagcct gcaccccacc gagttccaga
     1201 gcaccaagat ctacgaccgc cacggcgggc tgctctatga gatcagcgac ccgaccctgg
     1261 gcaagcgcac ctacctgggc atcgaacaac tgccgcccga cctgatcaac gccaccatcg
     1321 ccgccgagga cgccagtttc cgcgagaacg cgggcgtgga cctgcaaagc atcctgcgcg
     1381 ccgtctacat caacctgtcg ggccagggca gcagcggggc cagcaccatc accatgcaac
     1441 tggtgcgcgg ggccatgctg cccgaaaagg acgagccgac cttccgccgc aaggtgcgcg
     1501 aggcgatcct ggcgaaccgg gtaagcgaga cctacagcaa ggacaagatc ctcgaaatct
     1561 atctcaacga catctactac ggctcgcagg cctacggcgt gtccgcggcc tcgcaggtct
     1621 actacggcgt ggcggccaag gacctcgacc tggcgcaggc ggccatgctg gccgggctgc
     1681 cgcaggcgcc gtcctacttc gacccgcata ccaattaccg cgaggccaag atccgccagg
     1741 aatacgtgct cgatcagatg gtcaagtcgg gctacatcac ccaggcggag gcgaccaagg
     1801 ccgccgccga ggacgtgcat cccgtgccca cggtggccga ggccggcccc tcgcaggcgc
     1861 cccacttcgt caactatgtg cgcgccctgc tggataatat gtacggcccc gacatggtga
     1921 accggggcgg cttgcgcgtg ttcacgacca tcgacctggg cttccaggaa ctggcccgcc
     1981 gcaccgccgc cgcgcagatc
//
LOCUS       DASUYC010000612         2371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_468116,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000612 DASUYC010000000
VERSION     DASUYC010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468116"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VF276_09970"
     CDS             complement(<1..327)
                     /locus_tag="VF276_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607228.1"
                     /translation="MFWINRSRLVLPQVAAPDGTSRYLAEAGYELAVVECAADPFTEL
                     VLSWNAETPPGTAIRVEARLHYSPVDDQPEAGTWSAWRGLGHWGTTSLDATPLPRSGE
                     ATPPDDP"
     gene            563..832
                     /locus_tag="VF276_09975"
     CDS             563..832
                     /locus_tag="VF276_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607229.1"
                     /translation="MIQWAESAPPAALQGLMTDLRQTIQAECPALTVRVSYAPFQNMD
                     ALVEIRGAGAREAGLGAYLDATLQVAAAQGYTVALLMREPGGAGG"
     gene            1056..2111
                     /locus_tag="VF276_09980"
     CDS             1056..2111
                     /locus_tag="VF276_09980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX6607230.1"
                     /translation="MTVLARQRNKILGSVAFAILVYAIFLGLNDYNKLSQVLRNFTWG
                     LLPIILAFSLFNYALRFLKWHFYLHLVGVRTIGWLDSLIIFFSGFSMTITPGKAGEWL
                     KSLLVKQVAGTPVATTAPIILAERMTDGLAMLLLAGVGLFLFDNPLVRWFMVLVVVLA
                     VIATALVQNRALARRVGALMQRVQFLDKRVDHLRAFYNSAYELLRVRNLLLAIAIGFI
                     SWSGECIALFLILTGMGIPASWELLVLASFAMGTATLVGSVLLLPGGLGATEGSIEGL
                     LVFFGQPPWLPAGVISTSVGTAATLLIRFATLWFGVAIGFVALGIAQVRFGRLTVPDA
                     TGPATPDAEREAVGASR"
     gene            2108..>2371
                     /locus_tag="VF276_09985"
     CDS             2108..>2371
                     /locus_tag="VF276_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607231.1"
                     /translation="MSRAVSWAWAAACLLAAGLVLAACGNSNALLSGVSANPATVRPT
                     GLHPTEPIQLTYTLGRAATVTLHIQGGPDNAGLVLRPAEQLAAG"
BASE COUNT          352 a          779 c          794 g          446 t
ORIGIN      
        1 ggggtcgtca ggcggggtgg cctcgccgct gcgcgggagg ggcgtcgcgt ccaggctggt
       61 cgtgccccaa tgccccagcc cgcgccatgc cgaccaggtg ccggcttccg gctgatcgtc
      121 cacggggctg tagtgcaggc gggcttccac acggatggcc gtgccgggcg gggtctcggc
      181 gttccaggac agcaccagtt cggtaaaggg gtccgccgcg cactcgacca cggccagttc
      241 atagccggct tcggcgaggt agcgggatgt gccgtccggc gccgccacct ggggcaacac
      301 caaccggctg cgattgatcc agaacatgct gatggctcct tcaaggctcc tgctgagcag
      361 tcgcgttcag tatagcaagc gcccccgccg ctgacaaggc cgtatcgccc cacgaatccg
      421 tcgccatagc ggcctgttcg ccgaattgtc atgcctgccg ctcgcctgcg atccgccatt
      481 cgatacctat tcgagaggaa cgccatatta ctataaagag aggatgatca atgcggcagt
      541 agtaagaagg gaccgtgaac cgatgatcca atgggcagag agcgcgccgc cggccgcgct
      601 ccaggggttg atgaccgacc tgcggcagac gatccaggcg gaatgtcccg cgctgaccgt
      661 gcgtgtgagc tatgcgccgt tccagaatat ggacgccctg gtcgagatcc gcggcgcggg
      721 cgcccgcgag gccgggctgg gcgcctatct cgacgctacg ctccaggtcg ccgcggccca
      781 gggctacacc gtcgcgctgc tgatgcgcga accgggcggc gcgggcggtt agggccatcg
      841 gtttatcaat tcaccgcgaa ggcgcgaaga acgcgaagaa taaaacctgt attagtaacc
      901 ttcgtgttct tcgcgccttc gcggtgcaga atcagcgtat atgcacggat ggcaggcggg
      961 catttttgcg cccgacccgc gcgatcgggt ataatcgact attgcggacc gccgcatgat
     1021 tttacccgta tctagcatcc ctggagtaac cacctttgac cgttctcgcg cggcagcgta
     1081 acaagatcct gggttccgtc gcattcgcga tcctcgttta tgccatcttc ctcggcctca
     1141 acgactacaa caaactgtca caggtgttgc gcaacttcac ctggggcctg ttgccgatca
     1201 tcctggcttt cagcctgttc aactatgcgc tgcgcttcct caagtggcat ttctatctgc
     1261 acctggtggg cgtgcgcacg attggctggc tcgacagcct gatcatcttc ttcagcgggt
     1321 ttagcatgac catcacgccg ggcaaggcgg gcgagtggct gaagtcgctg ctggtcaaac
     1381 aggtggccgg gacgccggtg gcgaccaccg cgccgatcat cctggccgag cgcatgaccg
     1441 acggcctggc gatgctgctg ctggccgggg tgggcctgtt cctgttcgat aacccgctgg
     1501 tgcgctggtt catggtgctg gtggtggtcc tggccgtgat cgcgacggcc ctggtgcaaa
     1561 accgggcgct ggcccgccgc gtgggcgcgc tgatgcagcg ggtgcagttc ctcgacaagc
     1621 gtgtggacca tctgcgcgcg ttctataaca gcgcctatga actgctgcgc gtgcgcaacc
     1681 tgctgctggc gatcgccatc ggcttcatct cgtggtcggg cgagtgcatc gcgctgttcc
     1741 tgatcctcac cggcatgggc attcccgcct cgtgggagtt gctggtgctg gcctcgttcg
     1801 ccatgggcac ggccacgctg gtcgggtcgg tgctgctgct gccgggcggc ctgggcgcca
     1861 ccgagggcag catcgagggc ctgctcgtct tcttcggcca gccaccctgg ctgcccgcgg
     1921 gcgtgatctc cacctcggtg ggcacggcgg ccactctgct gatccggttc gccacgctct
     1981 ggttcggggt ggcgatcggt ttcgtcgccc tggggattgc ccaggtgcgc tttggccggc
     2041 tcacggtgcc cgatgcgacc gggccggcga ccccggacgc agagcgcgag gcggtcgggg
     2101 cctcgcggtg agtcgcgccg tctcctgggc ctgggcggcg gcctgcctgc tggccgccgg
     2161 gctggtgctg gccgcctgcg gcaacagcaa cgccttgctg agcggggtca gcgcgaaccc
     2221 ggccacggta cgccccaccg ggctgcatcc caccgagccg atccagctta cctataccct
     2281 gggccgcgcg gccacggtga cgttacatat ccagggtggc cccgacaacg ccggcctggt
     2341 gttgcgcccc gccgagcaac tggccgcggg c
//
LOCUS       DASUYC010000613         3429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_802187,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000613 DASUYC010000000
VERSION     DASUYC010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802187"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            487..1662
                     /gene="xylA"
                     /locus_tag="VF276_09990"
     CDS             487..1662
                     /gene="xylA"
                     /locus_tag="VF276_09990"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HEX6607232.1"
                     /translation="MADPFVPQKSDKFTFGLWTVGNMGRDPFGEPVRPALDPVYIVHK
                     LAELGAYGVNFHDNDLVPFGATPAERDRIVRDFKQALQETGLVVPMATTNLFNHPIFR
                     DGAFTSSDAAVRAFALQKTMRAIDLGAELGAKLYVFWGGREGSETDAYRNPQDAIKYF
                     RDAINYLCDYAEAQGYGMRFALEPKPNEPRGDLYFPTVGHMLHFIATLDHPDMVGVNP
                     EFAHETMAGLNFVHAVAEALEAGKLFHVDLNDQIMGRYDQDFRFASENPKRAFFLVKL
                     LEESGYDGSRHFDAHAYRTEDEAGVWAFARGCMRSYLILKDKVQRFQADQEIQGLLND
                     LRAADSQYTGPAAGGGYSREAAEALQNQQFDVSALAARGRRYEELDQLVMELLLGVR"
     gene            complement(1826..>3429)
                     /locus_tag="VF276_09995"
     CDS             complement(1826..>3429)
                     /locus_tag="VF276_09995"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HEX6607233.1"
                     /translation="KRRHGFDAEVSRRDLHTTYLPAFRAQVAAGVEAVMGAYNRVNGD
                     PCCAHPYLIGEVLRGTWGFAGHVVSDCGAVTDIFSGHHTAATEAAAAALAVTRGCDLE
                     CGKTYRHLDEALAQGLLTEADLDRALTRVLTTRFKLGMFDPPEQVPYAATPLSVVGSD
                     EHRALAYEAAVKSVVLLKNRNNILPIRPDVKTIRVVGPTAADLSVLLGSYHGLNGTLT
                     SLLEGIVSRLPEGVGLEYRMGCQLLHPNTATQEWLLSPRSQPDVVIACMGVTPMLEGE
                     EGDAIASTEEGDRARIELPPVQAEFVRKLAQAGARVVLVLTGGGPIALGDLEDLVEAV
                     VFVWYPGEAGGTAVADVLFGQVTPSGKLPLTFPRSLADLPPFDDYSMRGRTYRYATAE
                     PLYPFGFGLSYTQFAYSNLRLAQDSLPAGADLAGTVEVANVGPRDGEEVVQVYASAPD
                     GRGAPQHSLVAFERVALAAGARQTVAFTVPAARLTVVDAEGVAQAVPGPWRLTVGGCS
                     PGARGVALGAPEPATAGFTVTAPGA"
BASE COUNT          566 a         1185 c         1153 g          525 t
ORIGIN      
        1 tcagcgcgta ggacacggtg ctgagggcca cgcccgctcg tttcgccaca tcctgcatgg
       61 tcggcatcgc catggcacac ctcaaaagac aggatacgat ctagtactcg cgctacccat
      121 gtagtcgaag cgtgtagtcg aagcgtgtag tcgaaacgct tcgatgaaac gatgatagca
      181 ccatttcccc ggcctgtcaa taggtggcca gcataaattg aacccattcc gtcgattccg
      241 gtccattggg cccggaatag tcggtggctc ctactcgtat aaccccggca gcaaggcccg
      301 aatcctgctc cgaatccgtt gacgcatgac cgaggaagcc cagggtcttg acaggacagc
      361 gggccgggcc tatactcctg cccgtgtaag cgcttccatc gggcggaccc ggtatgagaa
      421 gcgtccgatc atgccatccg tggactatgt ttgccgcaaa ggcgtagcta agagaggagt
      481 tcgagcgtgg ccgatccgtt tgtgccgcaa aagagcgaca aattcacctt tggtctctgg
      541 accgtcggca atatggggcg tgaccccttc ggcgagccgg tgcgcccggc gctggacccg
      601 gtctacatcg tgcataagct ggccgaactg ggcgcgtatg gcgtgaactt ccacgacaac
      661 gacctggtgc ccttcggcgc cacgcctgcc gagcgcgacc gcatcgtgcg cgacttcaag
      721 caggcgctcc aggaaaccgg cctggtcgtg ccgatggcga cgacgaacct tttcaaccac
      781 ccgatcttcc gcgacggggc cttcacctcg tccgacgcgg cggtgcgcgc cttcgccctg
      841 cagaagacga tgcgcgctat cgacctgggg gccgaattgg gggcgaaact atacgtgttc
      901 tggggcgggc gcgaggggtc ggagacggac gcctaccgca acccgcagga cgcgatcaag
      961 tacttccgcg atgcgatcaa ctacctgtgc gactacgctg aggcgcaggg ctacggcatg
     1021 cgctttgcgc tggaaccgaa gcccaacgag ccgcgcggcg atctctactt cccgaccgtg
     1081 ggccacatgc tccacttcat cgccacgctg gaccaccccg acatggtggg cgtgaacccc
     1141 gagttcgccc acgagacgat ggccgggctg aacttcgtgc acgcggtggc cgaggcgctg
     1201 gaggccggga agctgttcca cgtcgacctc aacgaccaga tcatggggcg ctatgaccag
     1261 gacttccgct tcgcgtccga gaaccccaag cgggcgttct tcctggtgaa gctgctggag
     1321 gagagcggct acgacgggtc gcgccacttc gacgcccacg cctaccgcac cgaggacgag
     1381 gccggcgtct gggcgtttgc ccgcggctgc atgcgctcct acctgatcct caaggacaag
     1441 gtgcagcggt tccaggccga tcaggagatc cagggcctgc tcaacgacct gcgcgccgcc
     1501 gacagccagt acaccggccc ggcggcgggc ggcggctaca gccgcgaggc ggcggaggcg
     1561 ctccagaacc agcagttcga cgtgtccgcg ctggccgcgc ggggccggcg ctatgaagag
     1621 ctcgatcaac tggtgatgga actgctgctc ggcgtgcgct agcgtggcgt ccagccacct
     1681 attggaggct gcacactaaa gacacacgag cacggcgcga taaaccgcgc cgctacatcg
     1741 ggagtgttct ggctcagacc gcaagacgcg cccgccggcg ggtccgaatc gggcttccgc
     1801 cggcgggcgc gcgggtaaca ccggatcagg cgccgggcgc cgtcacggtg aaccctgccg
     1861 tcgcgggttc gggggcgccc agggccacgc cccgcgcgcc cggcgaacac ccgcccacgg
     1921 ttagccgcca cgggccgggc accgcctggg ccacgccctc ggcatcgacc accgtcaggc
     1981 gggcggcggg caccgtgaag gccaccgttt gccgcgcccc ggcagccagg gccacgcgct
     2041 cgaaggcgac caggctatgc tgcggcgccc cccggccgtc gggcgcgctc gcgtagacct
     2101 gcacgacctc ctcgccgtcg cgcggcccca cgttggcgac ctccactgtg ccggccagat
     2161 cggccccggc ggggagcgag tcttgcgcca ggcgcaagtt actgtacgcg aactgggtgt
     2221 agctgaggcc gaagccgaag gggtagagtg gctcggcggt cgcgtagcgg taggtgcgcc
     2281 cccgcatgct gtagtcgtcg aacggcggca ggtcggccag cgagcgcggg aacgtcagcg
     2341 gcagcttgcc cgacggcgtc acctgcccga acagcacatc ggcgacggcc gttccgccgg
     2401 cttcgcccgg ataccagacg aagaccaccg cctcgaccag atcctccaga tcgcccagcg
     2461 cgatgggccc gccgccggtc agcaccagca ccacgcgcgc ccccgcctgg gccagtttgc
     2521 gcacgaactc ggcctgcacc ggcggcaact caatgcgggc gcggtcgccc tcctcggtcg
     2581 aggcgatggc gtcgccttcc tcgccttcga gcatcggcgt cacgcccatg caggcgatga
     2641 ccacgtcggg ctgcgagcgc gggctcaata gccattcctg cgtcgccgtg ttggggtgca
     2701 ggagttggca gcccatgcgg tactccaggc cgacgccctc cggcagccgg ctcacgatgc
     2761 cttcgagcag cgaggtcagc gtgccgttca gcccgtggta gctgcccagc agcacgctga
     2821 ggtccgccgc cgtgggaccg accacgcgga tcgtcttcac gtccgggcga atcggcagga
     2881 tgttgttgcg gttcttgagc agcaccaccg atttgaccgc ggcctcgtag gccagtgccc
     2941 ggtgctcgtc gctgccgacc acgctgagcg gcgtggcggc atagggcacc tgctccggcg
     3001 ggtcgaacat gcccagcttg aagcgcgtgg tcagcacgcg ggtcagcgcg cggtcgaggt
     3061 cggcctccgt cagcaggccc tgggccaggg cctcgtccag gtggcggtag gtcttgccgc
     3121 actccaggtc gcagccgcgc gtgaccgcca gcgcggcggc cgcggcctcg gtggcggcgg
     3181 tatggtggcc gctgaagatg tccgtgaccg cgccgcagtc ggagacgacg tggcccgcga
     3241 agccccaggt cccgcgcagc acctcgccga tcaaataggg gtgggcgcag caggggtcgc
     3301 cgtttacccg attgtacgcg cccatcaccg cctcgacccc cgctgcgacc tgcgcccgga
     3361 acgcaggtag ataggtggtg tgcaggtcgc gccggctcac ctcggcgtcg aatccgtggc
     3421 ggcgctttt
//
LOCUS       DASUYC010000614         2132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_101285,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000614 DASUYC010000000
VERSION     DASUYC010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101285"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VF276_10000"
     CDS             <1..309
                     /locus_tag="VF276_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX6607234.1"
                     /translation="IVMAGRDIGTVVLPDAGRKIYLDASAQERARRRYLEQQGRPGAL
                     TYEQTLDEIVRRDTIDSTREVSPLRAAPAAIRIVTDGLSVEEVLDRALAAVGASPAAG
                     "
     gene            complement(496..783)
                     /locus_tag="VF276_10005"
     CDS             complement(496..783)
                     /locus_tag="VF276_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015187620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX6607235.1"
                     /translation="MRVTRTKIIRIGNSQGIRIPRRLLAQVGITPDAQGRFVGQEIEM
                     AVGAGGLILRPVSHVRAGWEEQFARMSEQSDDTLLYDATLANTWDEREWIW"
     gene            1169..1438
                     /locus_tag="VF276_10010"
     CDS             1169..1438
                     /locus_tag="VF276_10010"
                     /inference="COORDINATES: protein motif:HMM:NF023857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family antitoxin"
                     /protein_id="HEX6607236.1"
                     /translation="MTEANDTDQTPISHARTLDELAEFWDTHSLADYWDATQDAEFAV
                     RAERRHRVTLDPEVYAQIAALAQRRGLAPETLINLWLLERLQHAG"
     gene            1709..>2132
                     /locus_tag="VF276_10015"
     CDS             1709..>2132
                     /locus_tag="VF276_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607237.1"
                     /translation="MLTRTIAHPAGRYLLHAVAGLSLLATMAGGVLAPLPAAADPFAN
                     AAFQRLWERTDKPVAQGKVARSWLWGNEPFVAKQEPYAQGPNGQHLVQYFDKSRMEIN
                     DPNADPNSPWYVTNGLLVVEMMTGKIQIGNDAYQQVDPS"
BASE COUNT          386 a          728 c          667 g          351 t
ORIGIN      
        1 atcgtcatgg ccgggcgcga catcggcacc gtcgtcctgc ccgacgccgg ccgcaagatc
       61 tacctcgacg ccagcgccca ggagcgcgcc cgccgccgct acctggagca gcaaggccgc
      121 cccggcgccc tgacctacga gcagacgctg gacgaaatcg tccgccgcga caccatcgac
      181 agcacgcgcg aggtctcgcc cctgcgcgcc gcccccgccg ccatccgcat cgtcaccgac
      241 ggcctctccg tggaggaagt gctggaccgg gcgctggcgg cggtgggcgc ttcgccagcc
      301 gcggggtagg cgagcgcggg agggtttaca taatgccaac aggccccggc aatgccgggg
      361 ccttcttgcg cctggttcag attccagcta cttatcgtgt gtagctgcgc gatttatcgc
      421 gccgtactcg tttatcgtca gagtgtgctg ttctcctgag ttcgtcgacg gtgccctaat
      481 gcattcggca atagcctacc agatccattc ccgctcatcc caggtgttgg caagggtggc
      541 gtcgtacagc agcgtgtcgt cgctttgctc ggacatacgg gcgaactgtt cctcccagcc
      601 ggcgcgaacg tggctcacgg gacgcagaat cagaccgccc gccccgaccg ccatctcgat
      661 ttcttgcccc acgaaccggc cctgggcatc cggcgtgatc ccgacctgcg ccaagagccg
      721 gcgcggaata cggatcccct gcgagttgcc gatccggata atcttggtcc tggttacgcg
      781 catcgcgttg tcctcccgat aagcccggca ttacgtcgta actccattat ataccatggg
      841 tgcgccgggg tcagggagta gtttaggcgc cgggacctgt cattctgagc ggagcgaaga
      901 atctccgtct cgtgggggac caagacgaga ctcttcgctg cgctcagagt gacagccctt
      961 gtgtgactgc ctacgtggtg cagcgtccaa gcacctatgg aaggctgaac acgctacgac
     1021 caacgagtac ggcgcgataa atcgcgcagc tacacacaat atgtggctgg catcagcatt
     1081 aggacagatc ggcagccggg attgagccgt gcggcgccgc tgtgctacaa tagactatgc
     1141 tcacgccggg agaaggagcg caaacactat gaccgaggct aacgacaccg accagacccc
     1201 catctcgcat gcgcggacgc tggacgaact ggccgagttc tgggacacgc acagcctggc
     1261 cgattactgg gatgcgaccc aggacgcgga gttcgccgtg cgcgcggaac gccgccatcg
     1321 cgtgacgctg gacccggagg tctacgccca gatcgccgcc ctggcccaac gccgcgggct
     1381 ggcaccggaa acgctcatca atttgtggct gctggagcgg ctccagcacg cgggttagcc
     1441 tccggccctg cccgcagcga gactcattcc ccactacctg atccccgcat aaccaccgca
     1501 gccggtgccg caatgccctc agagggcgcc atacgcgggc acgcgacggg catgccgccg
     1561 tggcgcccgc ccgacccgac aggcgcaacc cccttcgtaa gtatccgttg aggatggtag
     1621 aggcaccatt cgaccattcc ctacaccggc gggcggggga gttgggtccg gagccgcccg
     1681 cagatggaga acctggaggt tcgtgacgat gttgacccgt actattgccc acccggcggg
     1741 gcgctatttg ctgcacgccg tggccgggct gtcgctgctg gcgaccatgg ccgggggcgt
     1801 gctggcgccc ctgcctgccg ccgccgaccc gttcgccaat gccgcgttcc agcgcctctg
     1861 ggagcgcacc gataagccgg tggcccaggg caaggtggcc cggagctggc tctggggcaa
     1921 tgagccgttt gtcgccaagc aggaacccta tgcccagggg ccgaacggcc agcacctggt
     1981 gcaatatttc gacaagagcc gcatggagat caacgacccg aacgccgacc ccaacagccc
     2041 ctggtacgtc accaacgggc tgctcgtggt cgagatgatg accggcaaga tccagatcgg
     2101 caacgatgcg taccagcagg tcgatccctc gc
//
LOCUS       DASUYC010000615         2994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_483953,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000615 DASUYC010000000
VERSION     DASUYC010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483953"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            346..1785
                     /locus_tag="VF276_10020"
     CDS             346..1785
                     /locus_tag="VF276_10020"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6607238.1"
                     /translation="MSRDNKGGKINRRTILRRVDQLGRIALLGLLLTACGGTAAEATT
                     TPVPQAAATTAPAAGATDTTAPAAAATDTTAPAAATNTSAPAASGGGDQVVNMLWTDT
                     DNLRQPRIAEFTKATGIKVNQVQVQYNELLDKINTTVQGGGDVDVIEMDTIWTAQFAS
                     AGWVEDLSARITDAQKKDIPQSSRSAVTYQGKLYGMPWYNIAKHLFYKSKLLQDAGFS
                     QPPATLDEFVKQAQATTKPGQWGSVWSWKQAESLICDWVAIMYTKKGAQILDDNGKAV
                     FNTQGGTEALQWMVDLLYKYKAADPASLEYTESDVQKAFRTGTVALTYNWEGDLPDDN
                     NPEKSKAAPNVKVGLLPGSADVRSSSVNGSEGWAILKQAKHKDAAWQLLQYMASPAWQ
                     KASALEAGDYPILSSLYSDPDLQQKVQDFPIYGEQFKYLAVRPQVPGYAQKSDIIQKH
                     LHEALLGKVSPKEAMDAAAAEVNAASNTP"
     gene            1813..2742
                     /locus_tag="VF276_10025"
     CDS             1813..2742
                     /locus_tag="VF276_10025"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX6607239.1"
                     /translation="MERTVVAPATRPARGGELSERALGLALLAPMALVLLLVVGYPLV
                     DSLWLSLHRANLANPEQGQPFIGLDNYIRAFGQPAFWYAIQRTLYFTVFSVGLELGLG
                     LLFAVLLNERFRGNLAARIAMILPWALLTVANGVLWAWILNPTYGVFNALLVDLGILQ
                     APKSWLSDTFWTMNMIILADAWKTVPNMTLLLLAGLQPISYDLYESADVDGASRWQKF
                     RYITLPLLRPVILVAVALRTIGAFRVFDIIYVLTGNGGPADSTKVISFYNYDQAFHYL
                     FFGYGAAISWLITAFMIVMILIYMRLLRSEDFA"
     gene            2758..>2994
                     /locus_tag="VF276_10030"
     CDS             2758..>2994
                     /locus_tag="VF276_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607240.1"
                     /translation="MRGNLLSPPVRWVLLLIYLLYTLVPMIWLFLSTIQTEASLLTLP
                     PRIIPSDVTLGNYVDIFKPAAFGENSGESTFLLAL"
BASE COUNT          524 a          991 c          977 g          502 t
ORIGIN      
        1 tgctcgtgca acggatcacc accggtgtgg cggcgccgga gatcgtgctg ccgacggagt
       61 tggtgatccg gcagtcgtgc gggtgtccct agcgggcggc ggccgcagtt atgccggagt
      121 ccgccgggcc ggtttgcaac cggcccctac agcagggtcg aggccgggtg gggcgcgcga
      181 atctgcccgc gccgcccacg ggcaccggct atcgttcggt gcctgatgcg gcccatcagg
      241 ctaccgttcc ttcgttgcct gatgcggccc atcaggccac cgttccttta cgggagcccc
      301 gcgcggcgcg ggctaggggt cagttagcag aaaggaacca gtgccatgtc tcgggacaac
      361 aaaggaggca agatcaaccg gcgtacgatc ctccgcagag tagaccagtt ggggcggatc
      421 gccctgctag gcctgctgct gacggcctgt ggcggcacgg ccgcggaggc caccaccacg
      481 ccggtgccgc aggcggcggc gaccactgcc ccggcggcag gggcgaccga caccaccgcc
      541 ccggcggcag cggccacgga taccaccgcc ccggcggcgg cgaccaacac cagtgccccc
      601 gcggcgagcg gcggcgggga ccaggtcgtc aacatgctgt ggaccgacac cgataacctg
      661 cgccagccgc ggatcgccga attcacgaaa gcgacgggga tcaaggtcaa ccaggtgcag
      721 gtccagtaca acgagttgct ggacaagatc aacaccaccg tccagggcgg cggcgatgtc
      781 gatgtgatcg agatggatac gatctggacg gcgcaatttg cctcggccgg gtgggtcgag
      841 gatctgagcg cccgcatcac cgacgcgcag aagaaagaca tcccccagtc gtcgcggagc
      901 gcggtgacgt accagggcaa actctacggt atgccgtggt acaatatcgc caagcacctg
      961 ttctacaaat ccaagctgct gcaggacgcc ggcttctccc agcccccggc cacgctggac
     1021 gagttcgtca agcaggccca ggcgaccacc aagcccggcc agtggggatc ggtctggtcc
     1081 tggaagcagg ccgagagcct gatctgcgac tgggtggcga tcatgtacac caagaagggc
     1141 gcgcagatcc tcgacgacaa cggcaaggcg gtgttcaaca cgcagggcgg caccgaggcg
     1201 ctgcaatgga tggtcgacct gctctacaag tacaaggccg ccgacccggc ctcgctggag
     1261 tacaccgagt ccgacgtgca gaaagccttc cgcaccggca ccgtggcgct gacctataac
     1321 tgggaaggcg acctgcccga cgacaacaat ccggagaagt ccaaggcggc cccgaacgtc
     1381 aaggtcgggc tgctgcccgg cagcgccgac gtgcgtagct cgagcgtgaa cggctccgag
     1441 ggctgggcca tcttgaagca ggccaagcac aaggacgcgg cctggcagct tctgcagtat
     1501 atggcgagcc cggcctggca gaaggcgtcg gcgctcgaag ccggggacta tccgatcctg
     1561 tcctcgctgt atagcgatcc cgatctacag cagaaggtgc aggacttccc gatctacggc
     1621 gagcagttca agtacctggc cgtccgcccg caggtgccgg gctacgcgca gaagtcggat
     1681 attatccaga agcatctgca cgaggccctg ctcggcaagg tcagtccgaa agaggccatg
     1741 gacgccgccg ccgccgaggt gaacgcggcc agcaacacgc cgtagcccgg cggtcacgca
     1801 aaggggttcg ccatggaacg caccgttgtc gccccggcca cccgcccggc tcgcggcgga
     1861 gagctgtccg agcgcgcact cggcctggcg ctgctggcgc cgatggccct ggtgctgctg
     1921 ctcgtcgtcg gctacccgct ggtcgactcg ttgtggctga gtctgcaccg ggcgaacctg
     1981 gcgaaccccg aacagggcca gccgtttatc ggcctggaca actatatccg cgccttcggt
     2041 cagccggcct tctggtacgc catccagcgc acgctctact tcaccgtgtt ttcggtgggc
     2101 ctggagttgg ggctgggatt gctcttcgcc gtgctgctca acgagcgctt ccggggcaac
     2161 ctggcggcgc gcatcgccat gatcctgccc tgggcgctgc tcaccgtcgc caacggcgtg
     2221 ctctgggcgt ggatcctcaa cccgacctac ggcgtgttca acgcgctgct ggtggacctg
     2281 ggcatcttgc aggcgcccaa gtcgtggctg agcgacacct tctggacgat gaacatgatc
     2341 atcctggccg acgcctggaa gaccgtgccc aacatgaccc tgctgctgct ggccgggctc
     2401 cagcccatct cctacgacct ctacgagtcg gcggacgtgg acggcgcctc gcgctggcag
     2461 aagttccgct acatcacgct gccgctgttg cggccggtca tcctggtggc ggtggcgctg
     2521 cgcactatcg gcgccttccg cgtgtttgac atcatttatg tgctcaccgg caacggcggc
     2581 ccggcggaca gcacgaaagt catctcgttc tacaactacg accaggcgtt ccactacctg
     2641 ttcttcggct acggcgcggc gatctcgtgg cttatcacgg cgttcatgat tgtgatgatc
     2701 ctgatctaca tgcgcctgtt gcggagtgaa gactttgcgt gaggtgttgc cgtgaccatg
     2761 cgaggcaatc tgctgagtcc cccggtgcgc tgggtgttgc tgctgatcta tctgctctac
     2821 acgctggtgc cgatgatctg gctgttcctc agcaccatcc agaccgaggc cagcctgttg
     2881 accctgccgc cgcgcatcat cccctcggac gtgacgctgg gcaactacgt cgatatcttc
     2941 aagcccgcgg cgttcggcga gaatagcggc gagtcgacgt tcctgctggc gctg
//
LOCUS       DASUYC010000616         3084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1249501, whole genome shotgun sequence.
ACCESSION   DASUYC010000616 DASUYC010000000
VERSION     DASUYC010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249501"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3084
                     /locus_tag="VF276_10035"
     CDS             <1..>3084
                     /locus_tag="VF276_10035"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6607241.1"
                     /translation="TVLVADLQGFTALTQALAAQPAGAEELRAALERIYTPLIAAVHA
                     HDGDVIKFTGDGVLAAFPDDITAARAAHAMLAGLDPVIETPAGAHPLVLRLGMGRGQL
                     TWEIVGTPDLGLYWVASGPALDSALAAEAGAAGLRPAPAGHMPTWTPPPPGEVPAAAD
                     SLAPYLPGAIVARMAGGLSGWVGEFRTLTAAFVALPAEGDLQTLVERALPLVARFGGR
                     LNEVEVGDKGRLLVILFGAPVAHGDDAARGAGCLLALREAGILARGGMAVGMQYVGTV
                     GAPGVARCTYTALGPEMNLAARLMQVAVPGEILISSRVWTALGLRFVCRERPALPVKG
                     WTAPVPVARLEAPEDAAGVPAIGDPLGRDSELAQAAAVIAGARAGPPGLLRLVGEAGI
                     GKSRLATTISNRAAGTGWRVLLGGGRPDRHSVPYWPWQAPLAVFITGRPEAPLTWPAV
                     EAAVARWAPARQADAPLLADLLGVSLPAAQAGPDAAPALDPATRAGATRTLLAALIAG
                     AARTQPLLIWLDDFHWADPLSWDLVRDLAAATPPGGPLVLLLSHRPFDAPPPALVALP
                     PHLALDLRELDSEAAAALVRQRATLPPSVVAEIVTRGQGHPFFLEELVAAYTDQAAPH
                     LPDTVQEVIQARIDRLDEAHKLTLKLASIFGRVFMLDALAGSYPPEIDAGAVPGHLQL
                     LAHLDLTPLTDAAVRTYAFKHAMTQEVAYASLLAAQRQAAHDRAGDWFERTTPDNYSL
                     LAYHYGLGANREKQRLYLRRAAETAQAAYANDSAIDFYERLLPLLAPAEQVPVLLQLG
                     GIHRLVGHIDTAEAVYRRALDQAKAAGDARAVAEAQMDLGVLERQRGAYTEALAWLEQ
                     ARTGFTRLGDASGLLAVQGHIGQVHWLQFDHPRALACFWEQMVLATDLGDRRGQGEAV
                     GNLGNVYDDLGDYAQALSCLEQQLDIARSIGDRQQQERAYWSRGSVYQNQRDFSRALA
                     DYGGAVRIAESIGDRRVVGLTVSGMGLIYHDYGDYAAALVCFIYHLAATLSLGNRV"
BASE COUNT          389 a         1228 c         1045 g          422 t
ORIGIN      
        1 accgtgctcg tcgccgacct gcaaggcttc acggccctga cccaggctct ggccgcccag
       61 cccgccgggg ccgaggagtt gcgcgccgcg ctggaacgca tctacacccc gctgattgcc
      121 gccgtccacg cccacgacgg cgacgtgatc aaattcaccg gcgacggcgt gctggccgcg
      181 ttccccgacg acatcacggc ggcgcgcgcc gcccacgcca tgctcgccgg gctggacccg
      241 gtgatcgaaa ccccggccgg cgcccacccg ctggtactgc ggcttggcat gggccgcggc
      301 cagctcacgt gggagatcgt cggcacgccc gacctcggcc tctactgggt cgcgtccggc
      361 ccggcgctcg acagcgcgct ggccgccgaa gccggggccg ccggattgcg ccccgcgccc
      421 gccgggcaca tgcccacctg gacaccgccg ccccccggcg aggtgcccgc cgccgcggac
      481 tcgctggcgc cctatttacc cggcgccatc gtggcgcgca tggccggcgg cctcagcggc
      541 tgggtgggcg agttccgcac gctcaccgcc gccttcgtcg ccctgcccgc ggagggcgac
      601 ctgcaaactc tggtggaacg tgccctgccg ctggtagccc ggttcggcgg ccgccttaac
      661 gaggtcgagg tgggcgacaa gggccgcctg ctggtcatcc tgttcggcgc gccggtggcc
      721 cacggcgacg acgcggcccg cggggcgggc tgcttgctgg ccctgcgcga agccggcatc
      781 ctcgcccgcg gcggcatggc cgttggcatg caatacgtcg gcacggtggg cgcgccgggc
      841 gtcgcgcgct gcacctacac tgccctcggc ccggagatga atctggccgc ccggctgatg
      901 caggtcgccg tccccggcga gatcctgatc agcagccgcg tctggacggc gctcggcctg
      961 cgcttcgtct gccgtgagcg cccggccttg ccggtcaagg gctggaccgc gccagtgccc
     1021 gtggcccgcc tggaagcgcc ggaggacgcc gccggggtgc ccgccatcgg cgacccgctg
     1081 ggccgcgaca gcgaactggc ccaggccgcc gccgtcatcg ccggggcgcg cgccggaccg
     1141 ccgggcctgc tgcggctggt gggcgaggcg ggcatcggca agagccgcct ggcgaccacc
     1201 atcagcaacc gcgcggcggg gacgggctgg cgggtgctgc tcggcggcgg gcgccccgac
     1261 cggcacagcg tcccgtactg gccctggcag gcgcccctgg ctgtgttcat caccgggcgc
     1321 ccggaagccc cgctcacctg gcccgccgtg gaggccgccg tggcccgctg ggcgcccgcc
     1381 cgccaggccg acgcgcccct gctggccgat ctgctcggtg tatcgctgcc tgccgcgcaa
     1441 gccggcccgg atgccgcgcc cgcgctcgac ccggccacgc gggcgggcgc gacgcgcacc
     1501 ctgctggccg cccttatcgc cggggccgcc cgcacccagc cactgctcat ctggctcgac
     1561 gatttccatt gggccgaccc gctctcctgg gacctggtcc gcgacctcgc cgccgccacg
     1621 ccgccgggcg gaccgctggt cctgctgctg tcgcaccggc ccttcgacgc gccgccgcct
     1681 gccttggtcg cactgccgcc ccacctggcg ctcgacctgc gcgaactcga ctccgaggct
     1741 gccgcggccc tcgtgcgcca gcgcgccacc ctgcccccat ctgtcgtcgc cgagattgtc
     1801 acccgcggcc agggccatcc cttcttcctg gaggaactgg tcgccgccta cactgaccag
     1861 gccgcgcccc acctgcccga caccgtgcag gaagtgatcc aggcgcgcat cgatcggctc
     1921 gatgaggccc acaagctgac gctgaagctg gcaagcatct tcgggcgcgt gttcatgctc
     1981 gacgcgctgg ccggcagcta tccgcccgaa atcgacgcgg gcgccgtgcc cggccacctg
     2041 caactgctgg cccacctcga cctgaccccg ctgaccgacg ccgccgtgcg cacctacgcc
     2101 ttcaagcacg ccatgaccca ggaagtggcc tatgccagcc tgctggccgc ccagcgccag
     2161 gccgcccacg accgcgcagg cgactggttc gagcgcacca cgcccgacaa ctactcgctg
     2221 ctggcctacc actacgggct cggcgccaac cgcgagaagc agcgcctcta cctgcgccgc
     2281 gccgctgaga ccgcccaggc ggcgtatgcc aacgactcgg ccatcgactt ttacgaacgc
     2341 ctgctgccgc tcctggcgcc cgctgagcag gtgcccgtgc tgctgcaatt gggcggcatc
     2401 caccgcctcg tcggccatat cgatactgcc gaggccgtct accgccgcgc gcttgaccag
     2461 gccaaggccg ccggcgatgc gcgggccgtg gccgaggcgc aaatggacct cggtgtgctc
     2521 gaacgccagc gcggcgccta taccgaggcc ctggcctggc tggagcaggc ccgcaccggc
     2581 ttcacccgcc tgggcgacgc ctccggcctc ctcgccgtcc agggccatat cggccaggtc
     2641 cactggctgc agttcgacca cccgcgtgcc ctggcctgtt tctgggagca aatggtgctg
     2701 gcgaccgacc tgggcgaccg gcgcggtcag ggtgaggccg tcggcaacct gggcaacgtc
     2761 tacgacgacc tgggcgacta tgcccaggcg ctctcctgcc tggagcaaca gcttgacatc
     2821 gcccgcagca tcggcgaccg gcagcagcag gagcgcgcct actggagccg agggtcggtc
     2881 taccagaacc agcgcgactt tagccgcgcc ctcgccgact acggcggggc agtgcgcatc
     2941 gccgagtcca tcggggaccg gcgcgtggtc gggttgaccg tgtcgggcat ggggctgatc
     3001 tatcacgact acggcgatta cgcggcggcc ctggtgtgct tcatctacca cctggccgcc
     3061 acgctgagcc tgggcaaccg cgtc
//
LOCUS       DASUYC010000617         3833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_191889,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000617 DASUYC010000000
VERSION     DASUYC010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191889"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1773
                     /locus_tag="VF276_10040"
     CDS             <1..1773
                     /locus_tag="VF276_10040"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX6607242.1"
                     /translation="TNPYGVPFSGPAQPAPRRNTAPLPDAPVFSAPPAPPAIYPYTPA
                     QAAPAPAPTRTKRANGGVGGLLLAGMLTVALVAGGVGGAGGAWLLTQNGASPSSAVTA
                     PPPAAKEAPVPAAVNPTGVADQNPSTIHDLYTRVSNSVVNIQVSTGGRFGEGGEGSGI
                     VVDKNHVLTNYHVVQGADTIRVNLIDNTSITAKLVGSAPQDDLAVLEADLPADKVQPA
                     VLGDSDSAQVGDEVVAIGNPFGLDHTVTAGIISAVNRPWADTGKPVRNMIQTDAPINP
                     GNSGGPLFNLQGQVIGITTAIESPVRGSVGVGFAIPINRAKSLLPQLTQGATVQRVWL
                     GISGLALDADTATQLKAPVNKGVLVTSVVPGAPADKAGVQGVDPTTDNSKLGDIITAV
                     DGNAVSQVQDITAYLDNKKVGDTVTLAINRNGQTVEVKVTLQAWPEQQQSQNSPVPQA
                     PNGQPGRPQGRVQAYLGIRGAALDDTLAKQLNVPITKGVVVTSVVDGSPAAKAGLQAA
                     PSDSKAGDIITAVDGQAVSSTADIVSALQNKKSGDSVTLSVWRDGQTRDVKVTLAEAP
                     ADGGQQPFPFPGFPSDPNGSPNTP"
     gene            complement(1880..2371)
                     /locus_tag="VF276_10045"
     CDS             complement(1880..2371)
                     /locus_tag="VF276_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607243.1"
                     /translation="MFGLSGRDWWLAAAETVPAVRPGARNALWAAAPRAIDGLAALFR
                     LPGLGRGAVALVARLGRHDPASAAAAGAVLRPLVAVEDASLRRAGVAALAQVADVYPG
                     AVAGPWRWRVEVALAAQADPRLTPDAARLLARTARRRRPVPAREAHLWAELARRGGPD
                     RVA"
     gene            2774..>3833
                     /locus_tag="VF276_10050"
     CDS             2774..>3833
                     /locus_tag="VF276_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607244.1"
                     /translation="MLKDLSSRRVRQWTALGISLLLIFLAVGAVKALPSAAARQLAAP
                     AGTKTGTVLGTANGFTMAQDAQHDTTPPLRSMPLIAPIPRAENGDKDAFQAIERLGGV
                     PGKDSVLQNFFGKLGGIPNPTVHFDGMYNQWGPIPPDTNGDVGPNHYYQIVNTGIQIF
                     NKAGTSLYGPADISTLWQGFGGPCASRNDGDPILLYDTQADRWFVSQFTTAAPFGECI
                     AISASGDPLGSYHRYFFSLSQTDLYDYPHCGVWPDAYYCAFNVFLNGASFGGASVTAF
                     NRDQMLNGQTTTFLASPPIPSAASFMPADVDGHTLPPAGEPNIFTTISTAAQALQFFN
                     FHVDWANPGSSTFTSGGSV"
BASE COUNT          678 a         1433 c         1194 g          528 t
ORIGIN      
        1 accaacccct acggggtgcc gttcagcggg ccggcccaac ccgcgccgcg ccgcaatacg
       61 gcgccgctgc ccgatgcgcc ggtgtttagc gcaccgcctg cgccgcccgc catctatccg
      121 tatactcccg cgcaggccgc gcccgcgccc gcccccaccc gcacgaagcg ggcgaacggc
      181 ggcgtgggcg gcttgctgct tgccggcatg ctcaccgtgg ccctggtggc cgggggcgtc
      241 ggcggcgcgg gcggcgcctg gctgctgacc cagaacggcg cgagccccag tagcgccgtc
      301 acggcgccgc cgcctgctgc taaagaggca cctgtgcccg cggcggtcaa cccgaccggc
      361 gtggccgacc agaaccccag caccatccac gacctctaca cccgcgtgtc gaactcggtc
      421 gtcaatatcc aggtcagcac gggcgggcgt ttcggtgaag gcggtgaagg ctcgggcatc
      481 gtggtcgaca agaaccacgt cctcaccaac taccatgtcg tgcagggcgc cgataccatt
      541 cgcgttaacc tgatcgacaa caccagcatc accgccaagc tcgtcggcag cgcgccgcag
      601 gatgacctgg ccgtgctgga agccgacctg cccgccgata aggtccagcc cgccgtcctc
      661 ggcgactcgg acagcgcgca ggtgggcgat gaagtggtcg ctatcggcaa cccgttcggc
      721 ctcgaccaca ccgtcaccgc gggcatcatc agcgcggtga accggccctg ggccgatacc
      781 ggcaagcccg tgcgcaacat gatccagacc gacgccccga tcaaccccgg caactccggt
      841 ggcccgctgt tcaacctgca gggccaggtg atcggcatca ccacggcgat tgaaagcccg
      901 gtacgcggtt ccgtcggcgt gggcttcgcc atcccgatca accgggccaa gagcctactg
      961 ccgcaactga cgcagggcgc cacggtgcag cgcgtctggc tcgggatctc gggcctggcg
     1021 ctcgacgccg acacggcgac gcagttgaag gcgccggtca acaagggcgt gctggtcacc
     1081 agcgtggtgc ccggcgcccc ggccgataaa gccggcgtgc agggcgtcga tccgactacc
     1141 gacaacagca agctgggtga catcatcacc gccgtggacg gcaacgccgt cagccaggtg
     1201 caggacatca cggcctacct ggacaacaag aaggtcggcg acaccgtcac cctggccatc
     1261 aaccgcaacg gccagaccgt cgaggtgaag gtgacgttgc aggcgtggcc ggagcagcag
     1321 caatcgcaga acagccccgt gccgcaggcg ccgaacggcc agccgggccg cccgcagggt
     1381 cgcgtccagg cgtacctcgg catccgcggc gcagcgctcg acgacacgct cgccaagcaa
     1441 ctgaacgtgc cgatcaccaa gggtgtagtc gtgacctcgg tggtcgacgg tagccccgcc
     1501 gccaaggccg ggttgcaggc ggcgccgtcg gacagcaagg ccggcgatat catcaccgcc
     1561 gtggacggcc aggccgtgag cagcaccgcg gatatcgtga gcgcgttgca gaacaagaag
     1621 agcggcgaca gcgtaaccct gtcggtctgg cgggatgggc agacgcgcga cgtgaaagtc
     1681 acgctggccg aggcccccgc cgacggcggg cagcagccct tcccgttccc tgggttcccc
     1741 agcgacccga acggctcacc caacacaccg taagatcgga gttccgcccc cggccaacgc
     1801 cgggaccgtt tctccataaa agtaaggccc agccgctgac ggctgggcct tgctgctgcc
     1861 gcggggcacg gggccgcgct caggcgacgc ggtcgggccc gccacggcgc gcgagttccg
     1921 cccacaggtg cgcctcacgg gcggggaccg ggcggcggcg ccgcgccgtc cgcgccagca
     1981 gccgggcggc gtcgggcgtg agccgcgggt cggcctgcgc cgccagggcc acctcgaccc
     2041 gccagcgcca ggggccggcc accgcgccgg gatagacatc ggccacctgg gccagtgccg
     2101 ccacgcccgc ccgccgcaac gacgcgtcct ccacagcgac cagcgggcgc agcacggcgc
     2161 ccgccgccgc ggcactcgcc ggatcgtggc gccccaggcg ggcaaccagc gccacggcgc
     2221 cccggcccag ccccggcagc cggaacagcg ccgccagccc gtcgatcgcg cgcggagccg
     2281 cggcccaaag tgcattgcgt gcccccggcc tcaccgccgg gaccgtttcc gcagccgcca
     2341 accaccagtc cctgccgctc agaccgaaca tgggcgcatc ctccaggcct attgataaag
     2401 attggggatc gcggcgggtg cccgcctccc cttctttatc gactcgttcg tacaaaatgc
     2461 ggaagaaaag atccaatgtt cattggcaat taactctgaa cctagttgtt atccaagttg
     2521 caaatccgaa gaatattctc tataatgccg gggtaccggt ggagcctgtg agcagctacc
     2581 ttgccgcatg ggctgccgga atcgattcca gcaaccctgg aacacgacct gtctcctgca
     2641 gtccacgctg aaaggagctt tgccggaggg ctgatgcgct actccccgcc tggctgatcc
     2701 ccgtccggga tcggcttgcc cgcaccagag agctaacgag cacaccaggt ccatatcagg
     2761 aaggatgcaa ctaatgctca aggatctctc gtcaagacgg gtccggcaat ggacggcgct
     2821 ggggatcagc ctgctgctga tcttcctggc cgtcggcgcg gtgaaggcgc tcccctccgc
     2881 cgcggcccgc cagctcgccg cccccgccgg caccaagacc gggaccgtgc tggggaccgc
     2941 gaacggattc accatggccc aggatgcgca gcacgacacg accccgcccc tgcgcagcat
     3001 gccgctgatc gccccgatcc cgcgggccga aaacggcgac aaagacgcct tccaggccat
     3061 cgagcggctg ggcggcgtcc ccggcaaaga ttccgtcctc cagaacttct tcggcaaact
     3121 cggcgggatc cccaacccga cggtccattt cgacggcatg tataaccagt ggggaccgat
     3181 cccgcccgac accaacggcg acgtcggccc gaaccactat tatcagatcg tcaacacggg
     3241 catccagatc ttcaacaagg ccgggacctc gctctacggt cccgccgaca tcagcacctt
     3301 atggcagggc tttggcggcc cgtgcgccag ccgcaacgac ggcgacccga tcttgctcta
     3361 cgatacgcag gccgatcgct ggttcgtcag ccagttcacc acggcggcgc ccttcggcga
     3421 gtgcatcgcc atctcggcct cgggcgaccc cctcggatcg taccatcgct acttcttctc
     3481 gctgagccag accgacctct acgactaccc gcactgcggc gtctggcccg acgcctacta
     3541 ttgcgccttt aacgtcttcc tcaacggcgc gagcttcggc ggcgcctcgg tcacggcgtt
     3601 caatcgcgac cagatgctga acgggcaaac gaccacgttc ctggcgtcgc cacccatccc
     3661 cagcgccgcg agtttcatgc ccgcggatgt cgacgggcac acgctcccgc ccgcgggcga
     3721 acccaacatc ttcacgacca tcagcaccgc cgcccaggcc ctgcagttct ttaacttcca
     3781 cgtggactgg gccaaccccg gcagctcgac ctttacctca ggcggcagcg tcc
//
LOCUS       DASUYC010000618         2161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_963025,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000618 DASUYC010000000
VERSION     DASUYC010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963025"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..1650
                     /locus_tag="VF276_10055"
     CDS             223..1650
                     /locus_tag="VF276_10055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX6607245.1"
                     /translation="MNDTRSKAALSLVAVVALLFAFGLGAFSSYVFTEPSRAGSFPDA
                     DSSGGTVPRVPPAATPAATGSQDLNAQMQTFYEIVDLLQKESYYRPTDSQKLVYGATE
                     GMMKAVGDEYTRFLTPEETAIENAQLQGESFGGIGVYFKEVDNLPTIVGIIPNTPAAR
                     AGLRPKDVILKVDGRDITTLTLDDIRPLIRGKEGTQVRLTILRGKDAPFDVDVTRAII
                     DVPLVTTTIRPDGVAVFAVSSFGDKTNSDLDAGIREAKSKGAKGIVLDLRNNGGGLVD
                     QAQQMIGHFVSPSMGQKYDDTALYESFASDGSQDRAIPIIRKGETMYDLPMAVLINGG
                     TASASEIVAGALSDYGRATLMGEQSFGKGSVQSVHSFADGSSARITIAHWLTPKKDDI
                     NPHPTATPDSSATPTPLPTFTPTPVGGPATPVPGVTLTPTPPPVDRSRGITPAIEVPP
                     PSDADYAADRDPQLDRAAQFVLTGK"
     gene            1774..>2161
                     /locus_tag="VF276_10060"
     CDS             1774..>2161
                     /locus_tag="VF276_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607246.1"
                     /translation="MTTQHSRTRTLLSLGAVAALVAAFGLGALTRPLLIGAGPASSTG
                     AKVAGPAPAATAQANLAAEMQTFYEIVDLLQKESYYHPTDTQKLVYGADQGLMEAIGD
                     PYTRFETPQRSAISRANLEGTYGGIGV"
BASE COUNT          402 a          784 c          662 g          313 t
ORIGIN      
        1 gggcacccgc ggcgatagat ctattatacc agttctttgt acaacgcata accatccgcc
       61 agggtgcaaa gatgctgctg tggcagcgag caacggctcg tgcaggttga ctcgatgcgc
      121 aggcgtccct tatacttatc gacaataggc ggctccggcc tcttgcgagc cgccgctttt
      181 gttcgcgccg cggatgctgc gcacccgaag gagcctcgta ttatgaacga tacccgcagc
      241 aaagccgcgc tcagcctggt cgcggtggtc gcgctgctct ttgcctttgg tctcggcgcc
      301 ttcagcagtt acgtcttcac cgaacccagc cgggccggca gcttccccga cgccgacagc
      361 agtggcggta ccgtcccgcg cgtgcccccc gccgcgaccc ccgccgccac gggatcgcag
      421 gacctgaacg cgcagatgca gaccttctat gagattgtcg atctgctgca aaaagagtcc
      481 tactaccgcc ccaccgacag ccaaaagctg gtctacggcg ccaccgaggg catgatgaag
      541 gccgtgggcg acgagtacac gcgcttcctg acgccggagg aaaccgccat cgagaacgcc
      601 cagctccagg gcgagagttt cggcggcatc ggcgtctact tcaaagaggt cgataacctg
      661 ccgaccatcg tcggcatcat ccccaacacc ccggcggctc gcgccgggct gcggcccaag
      721 gacgttatcc tgaaagtcga tggccgcgac attaccacgc tgaccctgga cgatattcgc
      781 ccgctgatcc gcggcaaaga aggcacccag gtgcgcctga ccatcctgcg tggcaaggac
      841 gcgccgttcg acgtggacgt gacccgcgcc atcatcgacg tgccgctcgt caccacgacg
      901 atccggcccg acggcgtggc cgtcttcgcg gtcagcagct tcggcgacaa gaccaacagc
      961 gacctcgacg caggcatccg cgaggcgaaa agcaagggcg ccaagggcat tgtcctcgat
     1021 ctgcgcaaca acggcggcgg cctggtggac caggcgcagc agatgatcgg gcactttgtc
     1081 tcgcccagca tgggccaaaa atacgacgac accgcgctgt acgagtcgtt cgccagcgac
     1141 ggcagccagg acagggccat acccatcatc cgcaagggcg agacgatgta tgacctgccg
     1201 atggccgtgc tgatcaacgg cggcacggcc agcgcgtccg agatcgtggc cggcgccctg
     1261 tccgactacg ggcgggccac gctgatgggc gagcagagct tcggcaaggg gtcggtgcag
     1321 agtgtccaca gcttcgcgga cggctccagc gcgcgcatca ccatcgccca ctggctgacg
     1381 cctaagaagg acgacatcaa cccgcacccg accgcgacgc cggactccag cgccacgccc
     1441 acgccgctgc ccaccttcac gccgacgccc gtgggcggcc cggccacgcc cgtgccgggc
     1501 gttacgctca cgcccacgcc gccgccggtg gaccgcagcc gcggcatcac gcccgccatc
     1561 gaggtgcccc cgccgagcga cgccgactac gccgccgacc gcgacccgca gttggaccgc
     1621 gccgcgcagt tcgtcctcac cggcaagtag ggccggggtg tcggcaccca ccgcgggggc
     1681 gggccaatgt gtctgccccc gcaccaccgt aggggcggac cgctgtgtcc gccctgtctc
     1741 cgaaagaaac cacctgatgc aaggtagccg gcattgacaa cgcagcattc acgcacccgg
     1801 accctgctca gcctgggcgc ggtggcggcc ctggttgcgg ccttcggcct gggcgcgcta
     1861 acccgccccc tgctgatcgg tgccggtccc gcgtcgagca cgggcgccaa agtagccggc
     1921 ccagcgcccg ccgccaccgc ccaggccaat ctggccgccg agatgcagac cttctatgag
     1981 attgtcgatc tgctgcaaaa agagtcctac taccacccca ccgacacaca gaaactggtc
     2041 tacggcgccg accaggggtt gatggaggcc attggcgacc cctacacgcg ctttgaaacg
     2101 ccgcagcgca gcgccatcag ccgggcaaat ctcgaaggca cctacggcgg catcggcgtc
     2161 t
//
LOCUS       DASUYC010000619         2880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_62704,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000619 DASUYC010000000
VERSION     DASUYC010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62704"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1638
                     /gene="mazG"
                     /locus_tag="VF276_10065"
     CDS             <1..1638
                     /gene="mazG"
                     /locus_tag="VF276_10065"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEX6607247.1"
                     /translation="QIIVVGLGPGAWEQVTLEARDVLAAADTVWLRTVTHPTNDYLPK
                     HLQIESFDYLYEREASFAAIYRQIAESLVERAQAGDPARPLIYAVPGHPLVGEASVRH
                     LLPLARAAGVPVRIVAGLSFLEPVCTALELDPLQHGLQILDATELLEMGDPTPLSSAK
                     WLKPDPPPTEEAQREPFYFSPYALPVPPEAFPTLQGLSSMLPVLVTQVYNDSVAGAVK
                     LGLLEKYPADHPVGLVMAAGVPGQERVLHLPLAELDHGHGIDHLACVYLPPLDPLAHP
                     REVDSLIYLMGRLRGPGGCPWDREQSHDSLKRYLLEETYETLDALDSGDPALMAEELG
                     DLLLQIVFHAQVAVSEEEFTLGDVVHSIVTKLVRRHPHVFGDVTVENAEGVVSNWQTI
                     KSAEKQARRAAGGGDESPDADLEVTSLLRSVPRSMPALSAAHQISERAVKAGFEWDRM
                     EDVFAKVHEELEELRTAADPAQRQEELGDLLFTLVNVARWWGVDAEETLRAMNRKFVR
                     RFAAMERLALRDGKRLRGMPVAEMEPYWQAAKQAERA"
     gene            complement(1693..2232)
                     /locus_tag="VF276_10070"
     CDS             complement(1693..2232)
                     /locus_tag="VF276_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607248.1"
                     /translation="MNTPSTNVPGPAAPPTHDDRWIGGAILIGIGLLAFISQYGGPLV
                     GGFALLAAGLVFLVAGSMSRRIGLLIPGGILTGLGVGVALITGPFAAYSGEATGAVIL
                     LSFAGGWVLITALSAVFTDKLIWWPLIPGGVLGLVGASLLAGENGIWLLQALGWVWPL
                     VLIALGVYLIIRRNAPAHR"
     gene            2488..>2880
                     /locus_tag="VF276_10075"
     CDS             2488..>2880
                     /locus_tag="VF276_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607249.1"
                     /translation="MSLSLLPVMGRLFNMGAGPVSEVPAIQAAQAVTTTATAGSAVPP
                     AGAGNIPAGQAANPFSVDYLTQAAQPDMGLFSTLFLLLSAILLGGGLYYYFVGKNRWR
                     RVHKLNYRLANTWSVIAMSLGGLGVLFVL"
BASE COUNT          482 a          968 c          991 g          439 t
ORIGIN      
        1 caaattatcg tggtggggct ggggccgggg gcgtgggagc aggtgacgct ggaggcgcgc
       61 gatgtgctgg cagcagcgga caccgtctgg ctgcgcacgg tgacgcaccc gacgaacgac
      121 tatctgccca agcatctgca gatcgagagt ttcgactatc tctacgagcg cgaggcgagc
      181 tttgccgcca tctaccggca gatcgccgag agcctggtgg agcgcgcgca ggcgggcgac
      241 ccggcccggc cgctgatcta cgcggtgccc ggccacccgc tggtgggcga ggcgtcggtg
      301 cgccatctgc tgccgctggc gcgcgcggcg ggcgtgccgg tgcgcattgt ggccgggctg
      361 tcgtttctgg agccggtttg caccgcgctg gagctcgacc cgttgcaaca cggcttgcag
      421 atcctcgacg cgaccgaact gctggagatg ggcgacccga ccccgctctc gtcggccaag
      481 tggctgaagc ccgacccgcc gcccaccgag gaagcccagc gcgagccgtt ctacttcagt
      541 ccctatgccc tgccggtgcc gccggaggcg ttccccacgc tgcaaggact cagttccatg
      601 ctgcccgtgc tggtgacgca ggtgtacaac gacagcgtgg cgggcgcggt caagctgggc
      661 ctgctcgaaa agtatccggc ggaccacccg gtgggcctgg tgatggcggc gggcgtgccg
      721 gggcaggagc gcgtgctgca cctgccgctt gccgaactgg accacggcca cggcatcgac
      781 cacctggcct gtgtctacct gccgccgctc gacccgctgg cccacccgcg ggaggtcgat
      841 tcgctgatct acctgatggg ccggttgcgc ggccccggcg gctgcccctg ggaccgcgaa
      901 caaagccacg attcgctcaa gcgttacctg ctcgaagaga cgtatgaaac cctggatgcg
      961 ctcgactccg gcgacccggc gctgatggcc gaggaactgg gtgacttgct gctgcagatc
     1021 gtgttccacg cccaggtggc ggtatcggag gaagagttca ccctgggcga cgtggtccac
     1081 tcgattgtga ccaagctggt gcgccgccac ccgcacgtgt tcggcgacgt gacggtggag
     1141 aacgccgagg gcgtcgtcag caactggcag acgatcaaga gcgccgaaaa acaggcccgc
     1201 cgcgcggcgg gcggcggcga cgagtcgccg gatgcggacc tggaggtaac gagcctgctg
     1261 cggagcgtgc cgcgctcgat gcccgcactc agcgctgccc accagatctc ggagcgcgcg
     1321 gtcaaggccg ggttcgagtg ggaccgcatg gaagatgtgt tcgccaaggt ccacgaggaa
     1381 ttagaggagt tgcgcaccgc cgccgacccg gcccagcggc aggaagagct gggcgatttg
     1441 ctgttcacgc tggtgaacgt ggcccgctgg tggggcgtgg acgccgagga gaccctacgc
     1501 gccatgaacc gcaagttcgt ccgccgcttc gccgccatgg agcgcctggc tctgcgcgac
     1561 ggcaagcgcc tgcgcggcat gccggtggcc gagatggagc cgtactggca ggcggccaag
     1621 caagccgagc gggcctagcg gcgcggacgc cgcaggcagc ggaccgggcc gcccgccgcc
     1681 tgctctgctg ccttaccggt gcgccggcgc gttccgccgg atgatcaggt agacgccgag
     1741 cgcgatcagg accagcggcc agacccagcc gagggcctgc agcagccaga tgccgttctc
     1801 cccggccagc aaggaggcgc cgaccagccc cagcacgccg ccggggatca gcggccacca
     1861 gatcagctta tcggtgaaca ccgccgagag cgcggtaatg agcacccaac cgcctgcgaa
     1921 gctgagcagt atgacggcgc cggtcgcctc cccgctgtac gcggcaaacg gcccggtgat
     1981 cagggccacg cccacgccga ggccggtcag gatgccgccg gggatcagca ggccgatacg
     2041 ccggctcatg ctgccggcga ccaggaacac caggcccgcg gccagaaggg caaatccgcc
     2101 cacgagcggc ccgccatatt ggctgataaa ggccagcaac ccgatgccga ttaagatagc
     2161 cccgccgatc caccggtcgt cgtgcgtggg aggcgccgcg ggaccgggta cattggtaga
     2221 tggagtattc atagccactc ctttccctat ccagcatggc acacgtcacc ccgccaggcg
     2281 atcagcccat gccgccacct taagtatggc gcttttcgcg gcgctgccgc gtaacggcaa
     2341 cgcaacggcg ggataggatt tcaccacaac gaggcggggg accggatgag tcgggttgtc
     2401 agcgcgccgc aaacctgcta taatcgcggc agttttgggc cattcatatc cccatcgtgc
     2461 aacttacatc caggaggctc tactcgcatg agtttgtcgt tactgcctgt catgggcagg
     2521 ttgttcaaca tgggtgccgg acccgttagc gaggtgcccg cgatccaggc ggcgcaggcc
     2581 gtcaccacga cggccaccgc cggcagcgcc gtcccgcctg ccggggccgg gaacatcccc
     2641 gccgggcagg ccgcgaaccc gttcagcgtc gattacctga cccaggccgc gcaacccgac
     2701 atggggctat tttcgacgct gtttctgctg ctcagcgcca tcttgctggg gggcgggctg
     2761 tattactact tcgtgggcaa gaaccgctgg cggcgtgtcc ataagctgaa ctaccggctc
     2821 gccaacacct ggagcgtcat cgccatgagc ctgggcgggc tgggggtgct ctttgtcctc
//
LOCUS       DASUYC010000620         3483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_14908,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000620 DASUYC010000000
VERSION     DASUYC010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14908"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..2736)
                     /locus_tag="VF276_10080"
     CDS             complement(145..2736)
                     /locus_tag="VF276_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEX6607250.1"
                     /translation="MNSKTINILELPKVLARLAGYTAFSASRTAAQTLAPSTDLAEVR
                     RRQQHTTEAVRLLGLRPEIGLGGVHDIRDSVRRAGLGAALEPGEFLVIQSTMEAGREL
                     RAVILRTEEQKGGLPVLAALADGITPLPRLETAIRRTFDKDGNIPDSASPALGRIRAQ
                     VRIAHDRLMSKLQQIVNSSSALQEPIISMRGDRYVVPVKADFRSQVKGIVHDQSSSGA
                     TLWVEPMAVVELANDWRKLQLDEQEEILRILRELAREVAAEAGPLTTMVGILAQVDLA
                     LACAKYSDAIGGVEPALDVPSEATAHAGDDSKPSGGAPAPRLIAGGDVEPRGIFLRNA
                     RHPLLTGTVVPINVELGDRFRVLVITGPNTGGKTVALKTVGLLTLMAMCGMHIPADTG
                     SRLLVVERIFADIGDEQSIEQSLSTFSSHMSNIISILKELNNRSLVLFDELGAGTDPQ
                     EGAALARALIEHLLHSGALAICTTHYSELKAFAYSTPGVENASVEFDVETLAPTYKLT
                     VGLPGRSNALAIATRLGLSRDLVAQAQRFLNPEDVHVENLLEGIRREHEQAGEERAAT
                     ERARREMEARRRDLERQLRDIDRLKQEALVEARAQAEDELAELRDLIRRVRVESESSA
                     LTREWVQQQAQRLESAQKDLRARNRRKAAAPAPNAAPAPAPVAPLRPGDRVYVPSFDA
                     EGEVLTAPDASNTAEVQLGAFKLRVKVDDMERVGQSGDGWTWRTSDGGARSGEEASRS
                     GGVRAWGTETAPRRSGRPETVTASSLMQRAAAAQVPMEIDLRGYRAEEIEPALDPYLQ
                     DAALAGMPMVRIIHGKGTGVLRQVTRDILKKHPLVRGWAPGPVEQGGEGVTLAYFDKP
                     PATTSDQ"
     gene            complement(2857..>3483)
                     /locus_tag="VF276_10085"
     CDS             complement(2857..>3483)
                     /locus_tag="VF276_10085"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEX6607251.1"
                     /translation="FIKDLHVVDILLFLIIVGFAFLGFTKGTVKLFIILASIYLGFII
                     AAIYYLPVADLVHNLFNISLSRVAQIIAFLLINVFVAVVLTILLFQFFGHIEIEGRAG
                     ACIDRPIGMLLGFLTGILFTAILVILLEVPYTLYEDLRLAGDQAPLFGILHNWFSNSL
                     LVPVLVKGLPILMGSISPLLNGHEPPILIKPTSEAMPFLIGLVDSRLG"
BASE COUNT          564 a         1147 c         1187 g          585 t
ORIGIN      
        1 gcgatcaatc gcgccgctac acacaaatta ggccgaacgc gctgacggac aaccgccgtc
       61 agcatataag cgaggggcgg taccgtcttc tacaaacggc accgcccctc tcctcatacc
      121 ggattactga tcactgacca ctgatcactg atcactcgtc gtcgccggcg gcttgtcgaa
      181 gtaggccagg gtgacgcctt cgccgccttg ctcgaccggg ccgggcgccc agccgcgcac
      241 cagcgggtgc tttttcagga tgtcgcgtgt cacctggcgc agcacgccgg tgcccttgcc
      301 gtggatgatg cgcaccatcg gcatcccggc cagcgcggcg tcttgcaggt agggatcaag
      361 cgccggctcg atctcctcgg cgcggtagcc gcgcaggtcg atctccatcg gcacctgggc
      421 cgccgctgcg cgctgcatca gcgaggaggc cgtgaccgtc tcgggccgcc cgctgcgccg
      481 gggcgccgtc tcggtgcccc aggcgcgcac gccgccggag cgggaggcct cctcgccgga
      541 gcgggcaccg ccgtcgctag tgcgccaggt ccagccgtcg ccgctctggc ccacccgctc
      601 catgtcgtcc actttcacgc gcagcttgaa ggcacccaac tgcacctccg cggtattcga
      661 ggcgtcgggc gcggtcagca cctcgccctc ggcgtcgaac gagggcacat agacccggtc
      721 gccgggccgc agcggcgcga cgggcgcagg cgcaggggcg gcgttaggcg cgggcgcggc
      781 ggctttgcgg cggttgcggg cgcgcaggtc cttttgcgcg ctttccagcc gctgggcctg
      841 ttgctgcacc cactcgcggg tcagcgcgct cgattcgctc tcgacccgca cgcggcggat
      901 caggtcgcgc agttcggcca gttcgtcctc ggcctgggcg cgggcctcga ccagcgcttc
      961 ctgcttcagc cggtcgatgt cgcgcaactg gcgctccagg tcgcggcggc gggcctccat
     1021 ctcgcggcgg gcgcgctcag tggccgcacg ctcctcgccg gcctgttcgt gctcgcggcg
     1081 gatgccttcc agcaggttct cgacgtgcac atcctccggg ttgaggaagc gctgcgcctg
     1141 ggccaccagg tcgcgcgaga ggcccaggcg ggtggcgatt gcgagcgcgt tggatcggcc
     1201 cggcagcccg acggtcaact tgtaggtggg cgccagcgtc tccacgtcga actccaccga
     1261 ggcgttctcc acaccgggcg ttgagtaggc gaacgccttc aactccgagt agtgcgtcgt
     1321 gcagatcgcc agcgcgccgc tgtgcagcag gtgctcgatc agcgcgcggg ccagggcggc
     1381 gccttcctgc gggtcggtgc ccgcgcccaa ctcgtcgaac agcaccaggc tgcggttgtt
     1441 cagctccttg aggatgctga tgatgttcga catgtgcgac gagaaggtgc tcaggctctg
     1501 ctcgatggac tgctcgtcgc cgatgtcggc gaagatgcgc tcgaccacca gcaggcgcga
     1561 gccggtgtcg gcggggatgt gcatgccgca catcgccatg agcgtcagca ggccgaccgt
     1621 tttcagggcc acggttttac cgccggtgtt cggcccagtg atgaccagca cccggaagcg
     1681 gtcgcccagt tcgacgttga tcggcacgac ggtgccggtg agcaaggggt ggcgggcgtt
     1741 gcgcaggaag atgccgcgcg gctctacgtc gcccccggca atgagccgtg gggcgggcgc
     1801 gccgccggag ggcttgctat cgtcgccggc gtgggcggtt gcctccgatg gcacatccag
     1861 cgcgggctcg acgccgccga tagcatcgct gtacttggcg caggccagcg ccaggtccac
     1921 ttgcgccagg atgccgacca tggtggtcag cggcccagct tcggcggcca cttcgcgggc
     1981 cagttcgcgc aggatgcgca agatctcttc ttgctcgtcc agttgcagct tgcgccagtc
     2041 gttcgccagt tcgaccacgg ccatcggttc cacccagagc gtggcgccgc tggacgactg
     2101 gtcgtggacg atgcccttca cctggctgcg gaagtcggcc ttcaccggca ccacatagcg
     2161 gtcgccgcgc atgctgatga tcggctcttg cagggcgctg ctcgaattga cgatctgttg
     2221 cagcttgctc atcagccggt cgtgggcgat ccgcacctga gcgcggatgc ggcccagcgc
     2281 ggggctggcc gagtcgggga tattgccgtc cttgtcgaag gtgcggcgga tggcggtttc
     2341 gagccggggc aggggcgtga tgccgtccgc cagcgcggcc aggaccggca ggcccccttt
     2401 ctgctcctcg gtacgcagga tgaccgcgcg cagttcgcgc cccgcctcca tggtgctctg
     2461 gatcaccagg aactcgccgg gttcgagggc tgcgcccagg ccggcacgac gcacgctgtc
     2521 acggatgtcg tgcacgccgc ccagcccgat ctccgggcgc agaccgagca ggcgcaccgc
     2581 ctcggtggtg tgctgctggc ggcggcgcac ctcggccagg tcggtcgagg gcgcaagcgt
     2641 ctgggccgcg gtccggctgg cgctgaaggc cgtatagccc gccaggcgcg ccaggacctt
     2701 gggcaactcc aggatgttga ttgtcttaga attcacgcga ttgggtcctt ctagtaacgc
     2761 gcgataatca gcaagtcagg aaaaaggcgc ccccgacgat acgggcgcgc cctgggcgag
     2821 cgacggcacc ggcaagtgcg ccggttacgg gccgggttag cccagccggg aatcgactag
     2881 ccctattaag aacggcattg cctcactggt tggtttgatc aggatcggcg gctcgtgccc
     2941 gttgagcagc ggcgaaatgc tgcccatgag gatgggtaaa cctttgacca gcaccggcac
     3001 tagcagcgag ttgctgaacc agttgtggag gatgccgaac aatggcgcct ggtcgccggc
     3061 caggcgcaga tcttcgtaca gggtataggg cacttcgagc aagatgacca ggatcgccgt
     3121 gaacaggatg ccggtgagga agcccagcaa catgccgatg ggccggtcga tgcaggcccc
     3181 cgcccggccc tcgatctcga tgtggccgaa gaactggaac agcagaatcg tgagcacgac
     3241 cgccacgaac acgttaatca acaagaaggc gatgatctgg gccacgcgac tcaggctgat
     3301 attgaacaga ttgtgtacca aatcggccac cggcaagtaa tagatggcgg cgatgatgaa
     3361 gccgagataa atggacgcca ggatgatgaa caacttcact gttcccttcg tgaagcctag
     3421 aaaagcaaag cccacgataa tcaagaacag gaggatatcc accacatgca ggtctttgat
     3481 gaa
//
LOCUS       DASUYC010000621         3514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1425320, whole genome shotgun sequence.
ACCESSION   DASUYC010000621 DASUYC010000000
VERSION     DASUYC010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1425320"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VF276_10090"
     CDS             complement(<1..551)
                     /locus_tag="VF276_10090"
                     /inference="COORDINATES: protein motif:HMM:NF020933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HEX6607252.1"
                     /translation="MPYDHAEIARQIAASREQALAAHATRTDAAQDAAEIYLATPHAD
                     WERAVKDVRSKRLMGVPQTPLHEFPATPPAGPDYTVVATDSSFVPPDKHRGSYCYLIN
                     VGRVMVHYGEEPAAELDSTPTHCAEPLIEGEDWMVSGRVLQAQCALQELTELHRWAAQ
                     FGADLALLDGSLMQLGLALSPSE"
     gene            complement(573..1265)
                     /locus_tag="VF276_10095"
     CDS             complement(573..1265)
                     /locus_tag="VF276_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607253.1"
                     /translation="MSASEPRRPSGPVDVPAPLGAPVPLDVPPYGPEIRPRRPRRWPH
                     WMGGIIVGWLTGVATLLILAALSVHLAPHPGPVTGDTDIRLQISANYLNDAVQRRLEQ
                     NPPAVANIKTVKLDLGLTPDSSMVLTPTFDVAGLFQVSPSATNQLTVRDGKVAMEMIG
                     QPRLGDLQIPLDLLPFDLTGEVHKAVDEITNNVLLAELNDMLKAGSGGDAFDVMEVQT
                     TPDYLIVKLQRK"
     gene            complement(1272..1502)
                     /locus_tag="VF276_10100"
     CDS             complement(1272..1502)
                     /locus_tag="VF276_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607254.1"
                     /translation="MEKWSEDWREIHRRMPDIHQDEYTIEGLSHLLGIPRDVITHEIT
                     TGQLHANRLGGHTICISREAVLDWLQRRGAGI"
     gene            complement(1562..2803)
                     /locus_tag="VF276_10105"
     CDS             complement(1562..2803)
                     /locus_tag="VF276_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEX6607255.1"
                     /translation="MTRSNRQMIGLGVLAGVALGAMYAANRQYQYSKTGVPSMLNWRR
                     VRTIAQNMNPEPALDAAWHERWAAYYTDLVRRCEPLIAEEMGRALPQPVQNIMAFSRS
                     EWVAANIVNFEQLFAPLERLHQDSSNTRDLGTLLMTDLNQVVLSSELGVLLGYFARRV
                     LGQYDMSLLGREPLAAGRLYFVEPNIAGVQQERGLDADDFRLWIALHETTHAFQFEAY
                     PWVREYFNSVLEEYFQLITDDLTLLRNGTGGVSQFITRARTNLAAGESWIEVVMNPEQ
                     KRLFQKIQALMSVIEGYSNYIMNAVGARLLPSYEYIKQRIEERAAQRGPAEQLLIRIT
                     GLALKMEQYRMGEAFINAVAGERGVSFANLVWAGPDYLPTLDELRNHRAWIARVEQLP
                     APADSPPTRSLNTAPLPEDSL"
     gene            3143..>3514
                     /locus_tag="VF276_10110"
     CDS             3143..>3514
                     /locus_tag="VF276_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607256.1"
                     /translation="MTTPGSGADAPDDPRSSPRSDPSRSPAPGPSPNPPAAPTPPVNA
                     ADLRRQRVIRPLAPRPPEPPPAPPGGRNGIIRRLERDPPGARAAGSVIRPLLPLPPNM
                     PGSRDRKTGIIRPLLPLPPLPG"
BASE COUNT          533 a         1176 c         1219 g          586 t
ORIGIN      
        1 tgttcgctgg gcgacagggc caggcccagt tgcatcagcg agccgtcgag cagggccagg
       61 tcggcgccga actgggcggc ccagcggtgg agctcggtca gttcctgcaa ggcgcattgc
      121 gcctgcaaca cccgcccgct caccatccag tcctcgcctt cgatgagcgg ctcggcgcag
      181 tgggtgggcg tgctgtcgag ttcggcggcg ggttcctcgc cgtagtggac catgacccgg
      241 cccacgttga tcaggtagca gtacgagccg cggtgcttgt cgggcgggac gaacgacgag
      301 tcggtcgcca ccaccgtgta gtccggcccg gcgggcggcg tggcggggaa ctcgtgcaga
      361 ggcgtctgcg gcacgcccat caggcgctta ctgcgaacat ccttaacagc ccgctcccag
      421 tcggcgtggg gcgtggccag gtagatctcg gcggcgtcct gggcggcatc ggtgcgcgtg
      481 gcatgggcgg ccagggcctg ctcgcggctg gccgcgatct ggcgggcgat ctctgcgtgg
      541 tcgtagggca tggaactctc gtcgctaggc gcctatttgc gctggagttt cactatcagg
      601 tagtcggggg tcgtctgcac ttccatgacg tcgaaggcat ccccgccgga cccggctttg
      661 agcatgtcgt tcaattcggc cagcaacacg ttattggtga tctcgtccac ggccttatga
      721 acttcgccgg tcaggtcgaa gggcagcaga tcgagcggga tctgcaggtc gcccaggcgg
      781 ggctggccga tcatctccat cgccaccttg ccgtcgcgca ccgtcaactg gttggtggcg
      841 ctgggcgaga cctggaagag cccggccacg tcgaacgtcg gcgtcagcac catgctggag
      901 tcgggcgtca ggcccaggtc gagcttgacc gtcttgatat tggcaaccgc cggtgggttc
      961 tgttccagcc ggcgctgcac cgcgtcgttc agatagttcg cgctgatttg caggcggatg
     1021 tcggtgtcgc cggtcacggg gccgggatgg ggcgccaggt gcaccgagag cgcggccagg
     1081 atcagcagag tcgccacgcc ggtgagccag ccgacgatga tgccgcccat ccagtgcggc
     1141 cagcggcgcg ggcggcgcgg gcgaatctcc ggcccgtagg gcggcacatc cagcggcacc
     1201 ggcgcgccca gcggggcggg cacatcgacc gggccggagg gccgccgggg ttcggaagca
     1261 ctcacccggt gctagattcc cgcgccccgc cgctggagcc agtccagcac cgcctcgcgc
     1321 gagatgcaaa tggtatggcc gcccagccgg ttggcgtgca gttggcccgt ggtgatctcg
     1381 tgggtaatga catcacgggg aataccgagc agatgggaca gcccttcgat ggtatattcg
     1441 tcctggtgaa tatccggcat ccggcgatga atctcacgcc agtcctcact ccacttctcc
     1501 acgacggtac cctcctgtga ctccccgcgc agtgcgccgg tagcggcggc aagggcggct
     1561 actacaaact gtcctccggc aacggggcag tattcagcga ccgggtcggc ggggagtccg
     1621 ccggggccgg caactgctcc acgcgcgcga tccaggcccg gtggttgcgc agttcgtcca
     1681 gcgtcggcag ataatccggc cccgcccaga cgagattggc gaagctgacg ccgcgttcgc
     1741 cggccaccgc attaatgaac gcctcgccca tgcgatactg ctccatcttc agcgccaggc
     1801 cggtgatgcg gatgagcagt tgctcggccg ggccgcgctg cgccgcgcgc tcttcgatgc
     1861 gctgcttgat gtactcgtat gaaggcaaga gccgtgcccc gactgcattc ataatatagt
     1921 tgctgtagcc ttcgatcacc gacatcagcg cctggatttt ctggaacaat cgcttctgct
     1981 cgggattcat gaccacttcg atccagctct cgccggcggc caggttggtg cgcgctcgcg
     2041 tgatgaactg cgacacgccg ccggtgccgt tgcgcagcag cgtcaggtcg tcggtgatca
     2101 gttggaagta ctcttcgagc acgctgttga aatattcgcg cacccacgga taggcctcga
     2161 actggaaggc gtgggtcgtt tcgtgcaggg cgatccagag gcggaaatcg tcggcgtcga
     2221 ggccgcgctc ctgttgcacc ccggcaatat tgggttcgac gaagtagagc cgcccggcgg
     2281 ccaggggttc gcgccccagc aggctcatat catactggcc gagcacgcgg cgggcgaagt
     2341 agccgagcag cacgcccaac tcgctcgaca gcaccacctg gttcaagtcg gtcatgagca
     2401 gggtgcccag gtcgcgggtg ttcgagctgt cctggtgcag gcgctccagc ggggcgaaga
     2461 gttgctcgaa gttgacgatg ttggccgcga cccactcgga ccggctgaag gccatgatgt
     2521 tctgcacggg ctgcggcagc gcgcggccca tctcctcggc gataagcggt tcgcagcggc
     2581 ggacgagatc ggtgtagtag gcggcccagc gctcatgcca ggcggcgtcg agcgccggct
     2641 cggggttcat gttctgggcg atggtacgca cccggcgcca gttgagcatc gaggggacgc
     2701 ccgttttgct gtactggtac tggcggttcg ccgcgtacat tgcgcctaac gcaaccccgg
     2761 cgagcacccc caggccgatc atctgccgat tgctccgagt catactagtg tcctttctcg
     2821 cacgaggtcg ttcgtatgtt ccactataac cctttgcccc tgcttcgtca agaatcgcac
     2881 aggcctaacc cccggcccct gcccggcgcg ggcagggggg aaggacggcg ggccgaggcg
     2941 cggcggggcc taacccccgg cccctgcccg gcgcgggcag gggggaaaga cggcgggccg
     3001 aggcgcgggg ggctgttctt gcccgcgccc cccgtttgtg ctatagtggg cagcaaggat
     3061 gccgaaccgg cccgcgggcc ggccccggcc ggccatcacc acagcacgcg ggcgcacggg
     3121 cgcctgccca ggagcgggag cattgactac acccggcagc ggggcggatg caccggacga
     3181 cccccgatct tcccctcgct ccgatcccag ccgcagcccg gcgccggggc catcgccgaa
     3241 tccacccgcg gcccccaccc cgccggtcaa cgcggcggat ttgcgccgcc agcgggtcat
     3301 ccggccgctg gcgccccgtc cgccggagcc gccgcccgcg ccgcccggtg gccgcaacgg
     3361 aattattcgg cgcctggaac gggatccgcc gggggcgcgg gcggccggga gcgtgatccg
     3421 cccgctgctg ccgttgccgc ccaatatgcc gggcagccgc gaccggaaga cgggcattat
     3481 ccgcccgctg ctgccgttgc cgccgctgcc cggt
//
LOCUS       DASUYC010000622         2481 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1682551, whole genome shotgun sequence.
ACCESSION   DASUYC010000622 DASUYC010000000
VERSION     DASUYC010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1682551"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..386
                     /locus_tag="VF276_10115"
     CDS             135..386
                     /locus_tag="VF276_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607257.1"
                     /translation="MTIHARNLPTEGCPIPPPPPPDIRAAQRAAAQAHRIFDALCTDP
                     DAPFDAMYRAWDVAAAADSAYYTALRAQRVVATWQGVRP"
     gene            383..586
                     /locus_tag="VF276_10120"
     CDS             383..586
                     /locus_tag="VF276_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607258.1"
                     /translation="MKPEPQTAFPLHPEHITPADWERNNPYAQPTTDADWGGDWEAYQ
                     AAYERAQAERTRERWALDVGEGR"
     gene            587..943
                     /locus_tag="VF276_10125"
     CDS             587..943
                     /locus_tag="VF276_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607259.1"
                     /translation="MLVAHPTPREQVGAALEAARQPQASYQPPRLQARPLFLGACSPG
                     WIVDMLPGGWAIIAYRPTGLMTDAESTAVQRETLGQYEREIFTEAAGWRCMWSRRKEG
                     APALLVQRPEWTARDE"
     gene            936..1091
                     /locus_tag="VF276_10130"
     CDS             936..1091
                     /locus_tag="VF276_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607260.1"
                     /translation="MNRPYEDEDEPTAWERGVNCQPVFIDRESDEDQTQANGDEPARD
                     DWRRWDE"
     gene            1084..1341
                     /locus_tag="VF276_10135"
     CDS             1084..1341
                     /locus_tag="VF276_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607261.1"
                     /translation="MSEPMWAWSLSARDTLRAGLETYRLRFGCDPPVILMARPAATEL
                     EGIPIHAAAIRPASVVWFGLTCPVSKPGPAVRLYEQEVLVL"
     gene            1338..1601
                     /locus_tag="VF276_10140"
     CDS             1338..1601
                     /locus_tag="VF276_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607262.1"
                     /translation="MKELYPGEYADLQERAQNECEAATQAAARLAALPPGTPEYATAE
                     LDSYYGDLAAGHAIWNVLEFEQANGLPPTPIERFWQATPEVTS"
     gene            1598..1861
                     /locus_tag="VF276_10145"
     CDS             1598..1861
                     /locus_tag="VF276_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607263.1"
                     /translation="MTTLLITLAAILVVAALDAWGRTRKPAPPAAPVHRDYLAPDRPL
                     YGAQRLPTRPVRPYTADEEAIIATVWGDGTRVLARPASTPERN"
     gene            1865..>2481
                     /locus_tag="VF276_10150"
     CDS             1865..>2481
                     /locus_tag="VF276_10150"
                     /inference="COORDINATES: protein motif:HMM:NF015776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HEX6607264.1"
                     /translation="MTTALTTRHTGLVGPDQWTIMRDQAAILVKSGLLPSTVNTPEKA
                     VAIMLKGSELGIPPMYALSNIVVIQGKPTANAELMLALIYRDHGDGAIQFARSDNATC
                     TVSYSRRTWTERQTFSFSLDDAKQAGLLSNATWQKYPAAMLRARAISAVARMAFPDSI
                     GGMYTPEELGAAVAVNGDTGEIVIDAQEVPAESTPGRAVAVGERRA"
BASE COUNT          436 a          870 c          823 g          352 t
ORIGIN      
        1 ggtgtgcact tctggcgccg gcggctgacc gatgcagatc tgacagacgc gcaggtcgcc
       61 gcgtaccaga ccatcgacca agtatggaac gacctgctgg acgattggga cgctgggaag
      121 gagggtgcct agccatgacc atccacgccc gcaacctccc caccgagggc tgcccgatcc
      181 cgccgccccc gccgcccgac atccgggcgg cccagcgcgc ggcggcgcag gcgcatcgca
      241 tctttgatgc gctctgcacc gacccggacg ccccgtttga cgccatgtac cgcgcttggg
      301 acgtggccgc cgccgccgat agcgcgtact acaccgcgct ccgcgcccag cgcgtggttg
      361 cgacgtggca gggggtccgg ccatgaagcc tgaaccgcaa accgcattcc cgctgcaccc
      421 ggaacacatc acgcccgccg attgggagcg caacaacccg tatgcccagc ccacgacgga
      481 cgccgactgg ggcggcgatt gggaagccta ccaggcggcg tatgagcgcg cgcaagccga
      541 gcgcacccgc gaacgctggg cgctggatgt tggggagggc cgctagatgt tggtcgccca
      601 ccccacgccc cgtgagcaag tcggggccgc gctagaagcg gcccggcaac cgcaagcctc
      661 gtaccagccg ccgcgcttgc aagcccgccc gctgttcctg ggcgcctgtt cgcccggttg
      721 gatcgtggat atgctccccg gcggctgggc gattatcgcc taccgcccga ccggcctgat
      781 gaccgatgcc gagagcaccg ccgtgcagcg cgaaacgcta ggccagtacg aacgggagat
      841 ttttactgag gcggcgggct ggcgctgcat gtggtcccgc cgcaaggaag gcgccccggc
      901 cctgctggtg caacgcccgg aatggacggc ccgcgatgaa taggccgtat gaggacgagg
      961 acgagccgac cgcctgggag cgcggcgtga attgccagcc ggtgtttatc gaccgggagt
     1021 cggacgagga ccagacgcag gcgaacggcg acgagccggc ccgcgacgat tggcggcgct
     1081 gggatgagtg agccgatgtg ggcctggagc ctctccgccc gcgacacgtt gcgcgccggg
     1141 ctggaaacct accggctgcg gttcgggtgc gacccgcccg tgatcctcat ggcacggccc
     1201 gctgcgaccg agctagaggg cattccgatc cacgccgccg ccatccggcc cgcgtccgtg
     1261 gtgtggttcg ggctgacgtg cccggtcagc aagcccggcc cggcggtgcg cctgtatgag
     1321 caggaggtgt tggtgctgtg aaggaactgt atcccggcga atatgccgac ctgcaagagc
     1381 gggcgcaaaa cgagtgcgaa gccgccacgc aggcggcggc ccgcctggcc gccctaccgc
     1441 ccggtacgcc cgaatacgcc acggcggaac tcgatagcta ctatggcgat ctggccgccg
     1501 gacacgcaat ctggaatgtg ctggagtttg agcaggcaaa cgggttgccc ccgacgccca
     1561 tcgaacggtt ctggcaagcg acgccggagg taacgtcatg accaccctgc tgatcaccct
     1621 ggccgcgatc ctggtcgtgg ccgccctgga tgcgtggggc cggacccgca agcccgcgcc
     1681 gcccgccgcc ccggtgcacc gcgactacct cgcgccggat cgcccgctct atggcgccca
     1741 gcggttgccc acgcgccccg tacggcccta caccgccgac gaggaagcga ttattgctac
     1801 cgtttggggc gacggcacgc gcgtgctggc ccgccccgct agcaccccgg aaaggaactg
     1861 agccatgact accgccttaa cgacccgcca tacgggtctc gtcggccccg atcaatggac
     1921 aatcatgcgc gatcaggccg ctatcctcgt caagagtggc ctgctaccca gcaccgtcaa
     1981 cacgcccgaa aaagccgtgg cgatcatgct gaaggggagt gaactcggca tcccgccgat
     2041 gtacgcgctc tccaacattg tggtcattca aggcaagccc acggccaatg ccgagttgat
     2101 gctcgcgctc atttaccgcg atcatggcga cggcgcaatc cagttcgcgc gcagcgacaa
     2161 cgcaacgtgt accgtcagct acagccggcg cacctggacg gagcgccaga ccttcagctt
     2221 cagcctggac gatgccaagc aggccgggct gctcagtaac gcgacgtggc agaaataccc
     2281 cgccgccatg ctacgcgccc gcgcgatcag cgccgttgcc cgcatggcgt tccctgactc
     2341 catcggcggg atgtacacgc cggaagaatt gggcgccgcc gtggccgtca acggcgatac
     2401 aggggagatc gtcattgatg cgcaggaagt gcccgccgaa tcgactccag gacgcgctgt
     2461 agcggtcgga gagcggcgcg c
//
LOCUS       DASUYC010000623         3557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_125525,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000623 DASUYC010000000
VERSION     DASUYC010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125525"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..1487
                     /locus_tag="VF276_10155"
     CDS             252..1487
                     /locus_tag="VF276_10155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6607265.1"
                     /translation="MRISFVTTTGITEGFRHWPEAVQARALAHRGHAVHAVTYYEAGS
                     PIIGRRAETIDRVDVRRVAFNRAWYAPGLGPALAAQRPDVIHLWHLRNALNAGAAAWA
                     ARAGVPVVFTVVGPFHDPYLVDDRERPYAGQVHWDRLIYTPAQLLRTLVRERHPQRVW
                     QNYRMHAPLLRADRVIALSDHEVSVLGLMGVPNERIRRVPLWVDADYIRAIPAVPAAG
                     EPPDPVVPGDGPFVLFIGQLKIRKGYDLLARAMPIVLATCPGTRFVFAGQNPAQAAEL
                     EAICAANGSREALVLLGPVSEGAKVRLLRRAACLVYPTRYESFGLPPLEAMVAGCPVV
                     STDLPVVREMIQSGANGLLAPPEDPAGLAAAILRLLQQPSLRAALVAGGQTTAATRYD
                     EDRLVAAVEDVYHEMAGGA"
     gene            1509..>3557
                     /locus_tag="VF276_10160"
     CDS             1509..>3557
                     /locus_tag="VF276_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607266.1"
                     /translation="MGDMGARAESRVTAPDRVGTPPVAGAPDAAVAAPAARAAWWRAP
                     ALWAFLGALLLAAIYGRVAVLNLTSGVVGGDLDGYENMWNNWWVKAALLDLHRNPFYT
                     DYLYYPTGISLRYHTLNPFNGLITIPFNLTLGYVPTTNLLFLAALALTTFFCFLLIRD
                     LVGNPWSAFAGAALFTFANGNMVSFFALGQAEKLSMEWLPLYLFFLFRAVHGRPVWEP
                     GAPAPTRLDTRPWLYAGLAILTLIIMSLTDWQYVMFAVFATLLYFAFLLCTRRPWAEK
                     RRVFLRLAAIGGIYAVLVTPPLLLPMIREALDSPWLSVGYQATARAVDLTELLGPTLA
                     NPGYLALALAGLGLWAGWRAGQRALAGFWALAVVLFYLMALGPVLTVGGTRTGIPLPY
                     ALMQALPVFNIGRDPGRYNTIAMLGVAVLAALGLRFVFGWVAARRPALGRLVLPAVVT
                     ALFLAVSLSGFVAAAGAAKADPPRWPPFYEQIAHDPEPYAILQLPSFTEKGLGEDHYM
                     MYQVLHQKPIFGGRWARDHKLTNPNNFMKTASLFRNFWLLDYTPDWRKLYYPPRDFLQ
                     RTDYATQGRAILNYYHTRYIVLWKDALYPRWDEDAFQQLIGQVLGPGTAPYYEDDMMR
                     VYRVPDGPPAANPLTLDVGEGWFQAETRPDGTVYRLADSSNGQASDVVSMNLTQHPLA
                     A"
BASE COUNT          486 a         1252 c         1219 g          600 t
ORIGIN      
        1 cccacaggaa acggattttc atacgcgctg gtgtgccgta ggcacatgat tggctcagag
       61 tgacaaggcc aggcagagac gagactcttc gctgcgctca gagtgacaag ccctaccgtc
      121 gaccgtctcc tgacggctga cggctgacgg ctgaccactg accactgacc actgaccact
      181 gaccactgat cactgaccac tgatccaatt tgtgccgccc ggcggctttg tgttacaatt
      241 gtgggattgt tatgcggatc agctttgtca ccacgaccgg gatcaccgag ggatttcgcc
      301 actggccgga ggccgtgcag gcccgcgcgc tggcgcaccg cggccatgcc gtccacgccg
      361 tgacctatta cgaggccggg tcgccgatca tcgggcggcg ggccgagacc atcgaccgcg
      421 tggatgtgcg ccgggtggcg tttaaccgcg cctggtatgc gcccggcctg ggtccggccc
      481 tggcagccca acggcccgat gtgatccacc tctggcacct gcgcaacgcg ctcaacgcgg
      541 gcgcggcggc ctgggcggcg cgggccgggg tgccggtcgt tttcaccgtc gtcggcccgt
      601 tccacgaccc ctatctggtg gacgaccgcg agcggcccta cgcggggcag gtccactggg
      661 accggctgat ctacacgccc gcgcagctcc tgcggaccct cgtgcgcgag cgccacccgc
      721 aacgcgtctg gcagaactac cgcatgcacg cgcccctgct gcgcgccgac cgcgtgatcg
      781 cgctgtccga ccacgaagtg agcgtgctgg ggcttatggg cgttccgaac gaacgcatcc
      841 ggcgcgtccc gctgtgggtg gacgccgact acattcgcgc catccccgcc gtgcccgccg
      901 ccggagagcc gcccgacccg gtcgtgccgg gcgatgggcc gtttgtgctg ttcatcggcc
      961 agttgaagat ccgcaagggc tacgacctgc tggcgcgggc catgcccatc gtgctggcga
     1021 cctgccccgg cacgcggttc gtgttcgccg ggcagaatcc ggcccaggcc gccgaactgg
     1081 aggccatctg cgcggccaac ggcagccgcg aggcgctggt gttgctcggc ccggtgagcg
     1141 agggggcgaa ggtgcggctg ctgcggcgcg cggcctgcct ggtctatccc acgcgctacg
     1201 agtcgttcgg cctgccgccg ctcgaagcca tggtcgccgg gtgccccgtc gtctcgaccg
     1261 acctgcctgt ggtgcgcgag atgatccagt cgggcgcgaa cggcctgctg gcgccgcccg
     1321 aggacccggc ggggctggcg gcggccatcc tgcggctgtt gcagcaaccg tcgctgcgcg
     1381 cggcgctggt cgccgggggg cagaccacgg cggccacgcg ctatgatgag gaccggctcg
     1441 tcgccgccgt cgaagacgtg taccacgaga tggcgggcgg cgcgtgacgt gcgggtagag
     1501 gcagactagt gggcgacatg ggcgcgcggg cggaatcgcg ggtgacggcg ccggatcggg
     1561 tggggacgcc gcccgtggcc ggagcgccgg acgctgctgt tgccgccccg gcggcgcggg
     1621 cggcctggtg gcgggccccg gcgctgtggg ccttcctcgg cgcgctgctg ctggcggcga
     1681 tatatgggcg cgtggccgtg ctgaacctga ccagcggcgt ggtgggcggc gacctcgacg
     1741 gctacgagaa catgtggaac aactggtggg tcaaggcggc gctgctcgac ctgcaccgca
     1801 atcccttcta caccgactac ctctactacc cgaccggcat cagtctgcgc taccatacgc
     1861 tcaacccgtt caacggcctg atcaccatcc cgtttaacct gacgctcggc tatgtgccca
     1921 cgacgaacct gctgtttctc gccgccctgg cgctgaccac gtttttctgc ttcctgctga
     1981 tccgcgacct ggtgggcaac ccctggtcgg cgttcgcggg cgcggcgctc ttcacgttcg
     2041 ccaacggcaa catggtctcg ttcttcgcgc tgggccaggc cgagaagctg tcgatggagt
     2101 ggctgccgct ctacctgttc ttcctgttcc gcgccgtgca tgggcggccc gtctgggaac
     2161 ccggcgcccc ggcccccacc cggctggaca cgcgcccctg gctctacgcc ggactcgcca
     2221 tcctgaccct gatcatcatg tcgctgaccg actggcagta cgtgatgttc gccgtgttcg
     2281 cgaccctgct ctacttcgcg ttcctgctct gcacgcggcg accctgggcc gagaagcggc
     2341 gcgtcttcct gcggctggcg gccatcggcg gcatctacgc cgtgctggtg acgccgccgc
     2401 tgctgctgcc gatgatccgc gaggcgctcg actcgccctg gctgagcgtg ggctaccagg
     2461 ccacggcgcg ggccgtcgac ctgactgaac tgctggggcc gaccctggcg aaccccggct
     2521 acctggcgct ggcgctggcc gggctggggc tgtgggccgg ctggcgggcg gggcagcgcg
     2581 ccctggccgg gttctgggcg ctggccgtgg tgctgtttta cctcatggcg ctggggccgg
     2641 tgctgaccgt cggcggcacg cgcacaggca tcccgctgcc ctacgcgctg atgcaggccc
     2701 tgcccgtctt caacatcggg cgcgaccctg gccgctacaa cacgatcgcc atgctcggtg
     2761 tggccgtgct ggccgcgctg gggctgcgct tcgtgttcgg ctgggtggcc gcccgccgcc
     2821 cggcgttggg gcggcttgta ttgcctgcgg tggtcacggc cctgttcctg gccgtcagcc
     2881 tgagcggctt cgtggcggcg gcgggcgcgg ccaaagccga tccgccgcgc tggcccccgt
     2941 tctacgagca gatcgcccac gacccggagc cttatgccat cttgcagttg ccgtcgttca
     3001 ccgagaaggg gctgggcgag gaccactaca tgatgtacca ggtcttgcac cagaagccga
     3061 tattcggcgg gcgctgggca cgcgaccaca agctgaccaa ccccaacaac ttcatgaaga
     3121 cggcgagcct gttccgcaac ttctggctgc tcgactacac gcccgactgg cgcaagctct
     3181 attacccgcc gcgcgacttc ttgcagcgca ccgactatgc aacgcagggc cgggccatcc
     3241 tcaactacta ccacacgcgc tatatcgtgc tctggaaaga cgcgctctat ccgcgctggg
     3301 acgaggacgc cttccagcag ttgatcgggc aggtgctggg gccgggcacc gcgccgtact
     3361 acgaagatga tatgatgcgc gtctatcgtg tgcccgacgg cccgcctgcc gccaacccgc
     3421 tgacgctcga cgtgggcgag ggctggttcc aggccgagac gcgccccgac ggcacggtct
     3481 accggctggc cgacagcagt aacgggcagg cgtcggacgt ggtgtccatg aacctgaccc
     3541 agcacccgct cgcggcc
//
LOCUS       DASUYC010000624         3969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1426392, whole genome shotgun sequence.
ACCESSION   DASUYC010000624 DASUYC010000000
VERSION     DASUYC010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1426392"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="VF276_10165"
     CDS             <1..1047
                     /locus_tag="VF276_10165"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6607267.1"
                     /translation="RRTAAGAKALDAAGRLALLQDDYAVMRPRLEESVAVWRAVGDDQ
                     GLAYALANLGIATAYRNRDVSAGGHLLIEEAVDRFRATGDQWGLAFALDLMGDVTGLL
                     GGDDLTVALCKEESLATYRELGDQWGIATELSELGQLALRQGDYDTARTQLEEAVTLE
                     QVAGDRWFAAHSIRSLGDIAWHQGDLARAQALYNESLALYRELGDTLRASAALRNLGH
                     IARDQGDYGEAAILFRRSLRLVHDLGNEQTVALCLAALAGVAALQGRHERAARLFGAA
                     DALREASRGLQPAPDLAASERGQTVARAALGEAAFARAWDEGRALPVAEAVAYATRPG
                     DDARRPAVPLGSSS"
     gene            1250..>3969
                     /locus_tag="VF276_10170"
     CDS             1250..>3969
                     /locus_tag="VF276_10170"
                     /inference="COORDINATES: protein motif:HMM:NF019145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607268.1"
                     /translation="MVGNGLGVILGVLFVGLLILLLAIWLLWLLRRRPASTGAGSGDA
                     NSGPPDPGYPPPDGEFQVDPASPNVHVVRDAQGQVIHVEHIRAFGLAVAQADPRALAD
                     TYVRANAGLFDPELQLAPAEPIPGIFLNGEPPRLRFAAERSIRGRDGSTVVTYGQTVN
                     GLPIWQAGLAVTVNYHDGRRLRVNSAQSSLHPHPLIDPATPRDAQFVPDKLSLTTLTR
                     LLYPDNLPATEQIRINVRPRLLYYRYRPLERFATPPVAPPQAAAAVVAAGTSLRAVGS
                     LEPGPLALADGAPTEPFAEPNTVLPLAPVPQSIQTEVHYLVTEVLFATTEVRKDGLHP
                     DVNWRAFVEVRTGAVLYLRAAVAACFDDIFPPGPAPGAPQFVSAAGSVFAADPVTLTG
                     DDPFAPGRTPSDGTLNPQPVPVTLANVLTSNNGTQSLNGTWVTLQDLNSPVGNPPSDV
                     TSPFDFTYPAVAASNNFTAVNAYYHTDAMYRMVDQLGMLGDFFAAGQPVRVDAQGMGN
                     SPNALTFGDGTHTRVSKIYFGRGRPASDVGNAIDIRVVLHEFCHALLWARVQGPNLGF
                     AHSAGDSLAAILNDPCSQVVVANRYQTFPWIMLENPTWPGRFHGGSQRTVAAGWGWGG
                     TIDGNEQRGSSTSGGYDREQILSTTLFRIYEAIGGDPQNSLAVRQQAARYLAYLIIRA
                     IGTLPSATFTPTNSPDEFATALMIADTGTAPAGNPTGGAIPGGSINKIIRWSFETQGL
                     YQPPGAPTPVTSAGQPPVDVYIGQNRALYDLFRPDFYETGDLWNRRNPDGGTTHEDPV
                     PNQPNYVYVRVRNRGYQAATPVTISGFYAPQAPDLVWERAGGWQPMTPPLTPFTDPLG
                     AAGSGTDTAVVGPYQWTPDNSPQSCLLMTVSAPGDPSNIDAASPFPCAIGP"
BASE COUNT          597 a         1452 c         1340 g          580 t
ORIGIN      
        1 cggcgcaccg cggcgggggc gaaggcgctg gatgccgcgg ggcggctggc cctgttgcag
       61 gatgactatg cggtgatgcg gccccggctg gaggaaagcg tggccgtctg gcgcgcggtg
      121 ggcgacgacc agggcctggc ctatgccctg gcgaacctcg gcatcgccac ggcctaccgc
      181 aaccgcgatg tcagcgcggg cgggcacctg ctgatcgagg aggccgtgga ccggttccga
      241 gcgaccggcg accagtgggg cctggccttc gcgctcgacc tgatgggcga tgtgaccggc
      301 ctgctgggcg gcgacgacct gacggtcgcg ctctgcaaag aggaaagcct ggcgacctac
      361 cgcgagttgg gcgaccagtg gggcattgcc acggaactga gcgaactggg ccagcttgcg
      421 ctgcgccagg gcgactacga tacggcgcgc acccaactgg aggaagccgt gactctggag
      481 caggtggccg gcgaccgctg gtttgccgcg cactccatac ggagcctggg cgacatcgcc
      541 tggcaccagg gcgacctggc gcgggcgcag gcgctgtaca atgagagcct ggcgctgtac
      601 cgcgaactgg gcgatacgtt gcgcgcctcg gcggcgttgc gcaacctggg ccatatcgcc
      661 cgcgaccagg gcgattacgg cgaggcggcg atcctgttcc gccgcagcct gcggctggtc
      721 cacgacctgg gcaacgagca gacggtggct ctctgcctgg ccgcgctggc cggggtggcc
      781 gccttgcagg gtcgccatga gcgcgccgcg cggctgttcg gcgcggcgga tgcgctgcgc
      841 gaagccagcc gcggcctcca acccgcgccc gacctggccg catccgagcg cggccagacc
      901 gtcgcgcggg ccgccctggg cgaggcggcg ttcgcccgcg cctgggacga gggccgcgcc
      961 ctgccggtgg ccgaggccgt cgcctacgcc acccgccccg gcgacgacgc ccgccggccc
     1021 gctgtgccgc tgggttcttc ctcgtagtcg tgtggtgatg gtcgccgggc cggtttcaaa
     1081 ccggccccta caggtccgaa tacaagcgac tcctcgctgg cgtgtgtggc cgcgctggtt
     1141 gcggggctgc atccggtggc gggcatcccc gcatcccgct gccgtaacaa acccaggccc
     1201 gcgacaataa accaaccaga gtacccggtc cttcaaggga ggtgcaccta tggtaggcaa
     1261 tggactcggc gtcatcctgg gcgtgctgtt cgtggggcta ctgatcctgc tactcgcgat
     1321 ctggctgctc tggttgctcc ggcgccgccc ggcgagcacg ggcgctggct ccggcgacgc
     1381 caactccggc ccgcccgatc ccggttaccc gccacccgat ggcgagttcc aggtcgatcc
     1441 ggcctcgccc aatgtgcatg tggtccgcga cgcgcagggc caggtcatcc acgtcgagca
     1501 catccgcgcg ttcgggctgg ccgtcgccca ggccgatccc cgcgcgctgg ccgataccta
     1561 tgtgcgcgca aacgccggcc tcttcgaccc ggaactccaa ctggcgccgg ctgagccgat
     1621 acccggcata ttcctcaacg gcgagccgcc acggttgcga ttcgccgccg aacgctcgat
     1681 ccggggtcgg gacggcagca ccgtggtgac ctacggccag acggtgaacg ggctgccgat
     1741 ctggcaggcg ggcctcgcgg tgacggtgaa ctatcacgac ggccgccgcc tgcgggtcaa
     1801 cagcgcgcag agcagcctgc atccgcatcc tctgatcgac ccggcgacgc cgcgggatgc
     1861 gcaatttgtg ccggacaagc tgagtctcac gacgctgacc cgcctgcttt accccgacaa
     1921 cctcccggcc accgagcaga tccgcatcaa cgtgcgcccg cgcctgctgt actatcgcta
     1981 ccggcccctg gagcgcttcg ctacgccgcc ggtggcgccc ccgcaggcgg cagcggccgt
     2041 tgtggccgcc ggtacctctt taagggcggt gggaagcctc gaacccggcc cgctggccct
     2101 cgccgacggc gcgcccaccg agccgtttgc cgagcccaac acggtgttgc cgctggcgcc
     2161 ggtgccgcaa tcgatccaga ccgaggtgca ttacctggtc acggaagttc tattcgcgac
     2221 gacggaggtg cgcaaggacg gcctgcatcc cgacgtgaac tggcgtgcct tcgtcgaggt
     2281 ccgcacgggt gccgtcctgt acctgcgcgc ggcggtcgcg gcgtgctttg acgacatctt
     2341 cccgcccggc cccgcgcccg gtgcgcccca gttcgtgtcc gcggcgggca gtgtcttcgc
     2401 cgccgacccg gtgacgctga ccggcgacga cccgttcgcg cccggcagga cgccgtccga
     2461 cggcacactc aatccccagc cggtccccgt cacgctggcg aacgtgctta ccagcaacaa
     2521 cggcacgcaa agcctcaacg gcacctgggt gaccctgcaa gacctgaatt cgccggtcgg
     2581 caacccgccc agtgacgtga ccagcccgtt tgatttcacc tatccggcag tggccgccag
     2641 caacaatttc accgccgtca acgcctacta ccatacggat gccatgtatc gcatggtgga
     2701 ccaactgggg atgctgggcg actttttcgc cgcgggtcag ccggtgcgcg tggatgcaca
     2761 gggcatgggc aacagcccca acgccctgac tttcggcgac ggcacgcata cgcgggtcag
     2821 caagatctat ttcgggcggg gccgcccggc gtccgacgtg ggcaacgcca tcgatatccg
     2881 cgtggtgctg cacgagtttt gccacgcctt gctctgggcg cgcgtgcagg ggccgaacct
     2941 cggattcgcc cacagcgcgg gcgacagcct ggccgcgatc ctcaacgatc cctgctcgca
     3001 ggtggtcgtg gcgaaccgct accagacctt cccctggatt atgctggaga acccgacctg
     3061 gccggggcgc tttcacggcg gctcacagcg caccgtcgcc gcgggctggg gctggggcgg
     3121 gaccatcgac ggcaacgagc agcgcggcag cagcacgtcc ggcggctacg accgcgaaca
     3181 gatcctgtcc acgaccctgt tccgcatcta cgaggccatc ggcggcgatc cgcagaattc
     3241 cctggccgtg cggcaacagg ccgcgcgcta cctggcctac ctgatcatcc gcgccatcgg
     3301 cacgctgccg tccgccacct tcacgccgac caacagcccg gacgagttcg ccacggcctt
     3361 gatgatcgcc gacaccggca ccgcgccggc cggcaacccg accggcggcg ccatccccgg
     3421 cggcagcatc aacaagatca tccgctggag ctttgagacg cagggcctgt accagccgcc
     3481 gggcgcgccc acgcccgtga cgagcgccgg gcagccgccg gtcgacgtgt acatcgggca
     3541 gaaccgcgcc ctgtacgacc tcttccggcc cgacttctac gagaccggcg acctgtggaa
     3601 ccggcgcaac cccgacgggg ggaccacgca cgaggacccc gtgcccaacc agccgaacta
     3661 cgtctatgtg cgcgtgcgca accggggcta ccaggcggcc actccggtga cgatcagcgg
     3721 cttttacgcg ccgcaggccc ccgatcttgt gtgggaacgg gcgggcggct ggcagccgat
     3781 gacgccgccg ctcacgccct ttacggaccc gttgggcgcg gcgggcagcg gcaccgacac
     3841 cgccgtggtc gggccgtacc agtggacgcc ggataactcg ccgcagtcct gcctgctcat
     3901 gacggtgagc gcgcccggcg acccgagcaa tatcgacgcg gcgtccccct tcccctgcgc
     3961 gatcggccc
//
LOCUS       DASUYC010000625         2496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1513308, whole genome shotgun sequence.
ACCESSION   DASUYC010000625 DASUYC010000000
VERSION     DASUYC010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513308"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..2008
                     /locus_tag="VF276_10175"
     CDS             116..2008
                     /locus_tag="VF276_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX6607269.1"
                     /translation="MTFRCPYCAAVNRKGAVFCLECGEPLDGTLPASEVAGATDQRGW
                     RGFLAGREPWLGAGIVVLLVALAIFSAWWGSRDRQWNAYRQAEIARQDHRWAAAIAGY
                     HVAGNIADAPSRLLELTRLQARLQAAQAAVVAARQAGDWWDVAVGLRTILDVDPQAVD
                     ANQLLEARRRAGYIVYRVPRGPDSGLWWVGADGLNPRQFPGGPDISLHGLSPDGRWAV
                     YTIYRFHPWPPGRHELYVLDLTSGELHQVPIAPVADPAPNPVRFRDDGQGFWWVLGSH
                     WSYYDLTTHQVHPIAGPVLATDTHGNHLLLASTSITPRSDGLATTLLLAGPTGTQPRI
                     LQTEVGVIRGARFSPDGQFLAYQVRTVLDQRPVDHLVVYALTRADGTLASSLNPARGY
                     FIAGTYLLDATQDAAAPGSFQDAFLPQAANGPRQYVAQAPGQPVLRITGPRSVNRPLQ
                     ARDSTATRSLRTDSLVAPWRAAGPDLGLGSGLWVDSLRTDDAGLFAALRLRTASGQAV
                     DWLGFASTGRYSLYLAPDGQDSLGLYSVPVAGAQAGVGDLPAGALHLLSRTADPYAWS
                     RNTALSHDGSRILAIRGPGEVGAPPLTQPSAAGLWAVRPDGTDPTLLVPNVAEFWTAD
                     GWLAAP"
     gene            2047..2481
                     /locus_tag="VF276_10180"
     CDS             2047..2481
                     /locus_tag="VF276_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020400109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 domain-containing protein"
                     /protein_id="HEX6607270.1"
                     /translation="MTQAGYSGTPLLKKLGIKPSARVLLLNAPAGYLARLGPLPAGVS
                     LRAAPEDGLDFIQLFSRESAEVAAWLPRLKAALAPAGALWISWPKRATRIPTDLSENL
                     IRDMGLRAGLVDVKVCAVDDTWSGLKFVYRLKDRVPPSAERG"
BASE COUNT          338 a          904 c          863 g          391 t
ORIGIN      
        1 ccgctatatt aaatgccatg ggttgcatga caatttcgtc atcttttctg actggggcaa
       61 aactgaaacc ggcgccgctg cgtaataccg gcaagggtgt agggaggatg ggccgatgac
      121 cttccgttgt ccgtactgtg ccgccgtgaa tcgcaagggc gctgtgttct gcctggaatg
      181 cggcgaaccg ctcgacggca cactgcccgc gtccgaggtt gccggggcga cggatcagcg
      241 cggctggcgc ggcttcctgg ccgggcgcga gccgtggttg ggcgcgggta tcgtcgtgct
      301 gctggtggcg ctggcgatct tcagcgcctg gtggggcagc cgcgaccggc agtggaacgc
      361 ctatcgccag gccgagatcg cccgccagga tcaccgctgg gcggcggcca tcgccgggta
      421 ccacgtcgcg ggcaacatcg ccgacgctcc gagccggctg ctggaactga cccggctgca
      481 ggcgcgcttg caggccgccc aggccgccgt ggtcgccgcc cggcaggcgg gggactggtg
      541 ggatgtggcc gtgggcctgc gtaccatact cgatgtcgat ccgcaggccg tggatgcaaa
      601 ccagctgctc gaagcgcggc gccgcgccgg ctacatcgtc taccgcgtgc cgcgcggccc
      661 cgactccggc ctctggtggg tgggcgccga cgggctgaac ccgcgacagt tccccggcgg
      721 ccccgacatc tcgctgcacg gcctgtcgcc ggacgggcgc tgggccgtct acacgatcta
      781 ccgcttccat ccgtggccgc cgggccgcca cgagttatac gtcctcgatc tgaccagcgg
      841 ggagctgcat caggtgccta tcgcgccggt cgccgacccg gcccccaacc cggtgcgctt
      901 ccgggacgac ggccagggct tctggtgggt gctgggcagc cactggagct actacgacct
      961 caccacccat caggtccacc ccatcgccgg gccggtgctc gccaccgaca cccacggcaa
     1021 ccacctgctg ctggcatcca cctcgatcac gccgcgcagc gacggtcttg ccaccacgct
     1081 gttgctggcc ggtcccaccg ggacgcaacc gcgcatcctg caaaccgaag tcggggtcat
     1141 ccgcggcgcc cggttcagcc ccgacggcca gttcctggcc taccaggtgc gtaccgtgct
     1201 ggaccagcgc ccggtcgatc acctcgtcgt ctatgcgctc actcgcgcgg acggcaccct
     1261 ggcgagcagc ctgaacccgg cgcgcggtta ttttatcgcc ggcacttatc tgctggatgc
     1321 cacgcaggac gccgccgcgc cgggttcgtt ccaggacgcc tttttgccgc aggccgcgaa
     1381 cggcccgcgc cagtatgtcg cccaggcacc ggggcaaccg gtgttgcgca tcaccggccc
     1441 gcgcagcgtg aaccgccctc tacaggcgcg cgacagcacg gccacccggt ccctgcgcac
     1501 cgatagcctc gtcgccccct ggcgggccgc ggggccggat ctgggcctgg ggtcgggtct
     1561 gtgggtggac agcctccgca ccgacgacgc cgggctgttc gcggcgttgc ggctgcgcac
     1621 ggcgtcgggc caggcggtgg actggctcgg cttcgcgtcc accgggcgct atagcctcta
     1681 cctggccccg gacgggcagg acagcctggg cctctacagc gtgccggtgg ccggcgcgca
     1741 ggccggtgtc ggcgacctac ccgccggggc gctgcatctc ctgagtcgca cggcggaccc
     1801 ctacgcctgg agccgcaata ccgccctcag tcacgacggc agccgcatcc tggcgatccg
     1861 cgggcccggc gaggtcgggg cgcctccact gacccagccc agcgcggcgg gcctctgggc
     1921 cgtgcgcccc gacggcaccg acccgaccct gctcgtcccc aatgtggccg agttctggac
     1981 ggcggacggc tggctggcgg cgccttaggc acccgcgcac ggtccgtttc ctgatcggag
     2041 atcctcatga ctcaggcggg ttactccggc acgccactgt tgaagaagct aggcatcaag
     2101 ccgagcgcac gggtgctgct gctcaacgcg cccgcgggct acctggcgcg gctcggcccg
     2161 ctgcccgcag gagtatcgct gcgggctgcg cccgaggacg gactggattt catccaactc
     2221 tttagccgcg agtccgccga ggtggcggcg tggttgcccc ggctcaaggc ggcactggcc
     2281 ccggcgggcg cgctctggat ctcctggccg aaacgcgcca cgcgcatccc gaccgatctg
     2341 tcggagaacc tgatccgcga catgggtctg cgcgcggggt tggtggacgt gaaagtttgc
     2401 gccgtggacg acacctggtc gggcctgaag ttcgtctacc ggctcaagga tcgggtgccg
     2461 ccatctgcgg aacgcgggtg atgccgcgcg tccggc
//
LOCUS       DASUYC010000626         3379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_2050187, whole genome shotgun sequence.
ACCESSION   DASUYC010000626 DASUYC010000000
VERSION     DASUYC010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2050187"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VF276_10185"
     CDS             complement(<1..268)
                     /locus_tag="VF276_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607271.1"
                     /translation="MKHKRTFTASLLSGMLALAVCLGLSAGTPVPLQAQTKTGDFTFS
                     QTGFTVPSTFMDYWRSHGGLAVFGYPISPVMNEGGYQVQYFERNR"
     gene            complement(400..1896)
                     /locus_tag="VF276_10190"
     CDS             complement(400..1896)
                     /locus_tag="VF276_10190"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX6607272.1"
                     /translation="MTSLQSDSPAGPAETPDLLGASAAATQLRAVRARHVAHLRPELA
                     DSPSPIRSMARMVNDVVAEARELGIPEPVLAAHVVNRSIGDVDVRRITEIYNGVDYST
                     IGEDLGVILPGEVINGRVSDGATYLEIRRRMLEFERRLLADYGFDTHIYDLSGIGNPL
                     LRERLAARFRDHYGVPATAEQTFVSIGGLDAIDKSLRGFTYYFRQKYGGRVGFAFPAP
                     GFAVPLWQATSLDMDIINFPTREEDRFQLTPATMERLLATHPNLRVLYLTVSNNPTAF
                     AYSPADLRAVLDVIERDGRELAILADLAYVGTGVPEQDRARMAAFLTPETLQRTLFVN
                     SFSKVFTLTGDRMGYVSLANAEWVQFLTAVWNNANAGMPGDWQLRFLAYVSLFEERPW
                     IQAKIRDLYTLRRTALRAELAALNAQYHVFEQVGIDDHATVYNWSKLAPGEDLFTLFE
                     KTGIAGVDGRGFGYTPQYIRLSVGFIPVRPEQLAAMEQAAAGASQPPA"
     gene            2087..2596
                     /locus_tag="VF276_10195"
     CDS             2087..2596
                     /locus_tag="VF276_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX6607273.1"
                     /translation="MPTTEDLIMMLRVLTAFLLGGLVGWERERMQRPAGLRTHMLVAA
                     GAACFTVASIYGFAGEGTNRDPARLAAQIVSGIGFLGAGVIFRTGGSVRGLTTAASIW
                     IVAALGVLAGQGLYWVASFATVLTWFALRVLKSAERFGGDPDSAPDGEEDRDDGPPAA
                     PAGESTEPR"
     gene            2636..>3379
                     /locus_tag="VF276_10200"
     CDS             2636..>3379
                     /locus_tag="VF276_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607274.1"
                     /translation="MLTRGRALITAHPRAVLVALLLAGLLVRLPFAGIDTHLSNDIIV
                     FTQWARLLELRGLAAIYQDPSINYPPIPLYLLGGAALLDTHLFRGNTTALVALIKLPG
                     ILCDVATAALLAWTVQRRGRRVSLGVCALYLFNPGVWYVSALWGQFEAVYTLPIVAGV
                     VALERGRLVPAWVAAALALATKPQSLALAPVLVGASLARRGLRGLVLGAGAALLVAFG
                     LALPWLGAGNLLDAAGAYTNVRASPWLDVS"
BASE COUNT          515 a         1097 c         1198 g          569 t
ORIGIN      
        1 agcggttgcg ctcgaaatac tgtacctggt agccgccttc gttcatcacc ggggaaatcg
       61 gatagccaaa gacggccagc ccgccgtggc tgcgccagta atccatgaac gtggacggta
      121 cggtaaaccc ggtctggctg aaagtgaaat cgccggtttt agtctgggct tgcagcggca
      181 cgggcgtgcc ggcgctcagc cctaggcaca ctgccaacgc gagcatgccg ctaagcagcg
      241 atgcggtgaa cgtccgctta tgcttcacgc gaacctcctg gatatggaca tgtggactcc
      301 acggccagcg ccgggaagcc cgatgggtgg atagaaggca gggcgccgcg ccggcgaccc
      361 aggttcgtgg atgggccggt ggactccgat ggtggactcc tatgcaggtg gttgcgacgc
      421 gcccgcagcc gcttgctcca tggccgccag ttgctccggc cggacgggga tgaacccgac
      481 cgacagccgg atgtattgcg gggtgtagcc gaagccccgg ccgtccacgc ccgcgatacc
      541 cgttttttca aacagcgtga agaggtcttc gcccggcgcc agcttgctcc agttgtagac
      601 ggtggcgtgg tcgtcgatgc cgacctgctc aaacacgtga tattgggcgt tgagcgcggc
      661 cagttcggcg cgcagcgcgg tgcggcgcag ggtgtagaga tcgcggatct tggcctggat
      721 ccagggccgc tcctcgaaca gcgacacata ggccaggaag cgcagttgcc agtcgcccgg
      781 catcccggcg ttggcgttgt tccagaccgc ggtcaggaat tgcacccact cggcattcgc
      841 cagggagaca tagcccatcc ggtcgccggt gagggtaaag actttggaga agctgttgac
      901 gaacagggtg cgctggaggg tctccggggt gaggaacgcc gccatgcgcg cccggtcttg
      961 ctccggcaca ccggtgccga cataggccag gtcggccagg atcgccagtt cgcggccgtc
     1021 gcgctcgatc acgtcgagca cggcgcgcag gtcggcgggg ctgtaggcga acgccgtggg
     1081 gttgttgctc accgtcaggt agagcacgcg caggttgggg tgcgtggcaa gcagccgctc
     1141 catggtcgcc ggggtgagct ggaagcggtc ttcctcacgg gtcgggaagt tgatgatgtc
     1201 catatccagg ctggtcgcct gccagagcgg cacggcgaag cccggcgcgg ggaaggcaaa
     1261 gcccacccgc ccgccatatt tctggcggaa atagtaggta aatccgcgca acgacttgtc
     1321 gatggcgtcc aggccgccga tactgacgaa cgtctgctct gccgtggcgg gcacgccgta
     1381 gtggtcgcgg aagcgggcgg ccaggcgttc gcgcagcagc ggattgccga tgcccgacag
     1441 gtcgtagatg tgcgtgtcaa agccgtagtc ggccagcagc cggcgctcga actccagcat
     1501 ccgccggcgg atctccaggt aggtcgcccc gtcactgacc cgcccgttga tgacctcgcc
     1561 gggcaagatc acgcccaggt cctcgccgat ggtgctgtag tccacgccgt tatagatttc
     1621 agtgatccgg cggacatcga catcgccgat gctgcggttg accacatgtg ccgcgaggac
     1681 cggctccggg atgcccaact cgcgcgcttc ggccaccacg tcgttgacca tgcgcgccat
     1741 gctgcggatc ggggagggac tatcggccag ttcggggcgc aggtgggcga catggcgggc
     1801 gcgcaccgcc cgtaactgcg tggcagccgc ggacgcgccg aggaggtcgg gcgtctcggc
     1861 cggaccggca ggggaatccg attgtaaact ggtcatggat gctcctggat ctgggacggc
     1921 ataccggtgt ctatcgccgg ggcgaggggc aaaacggcac gcccggcgta agtatatgcg
     1981 ctggacaggc gctttgtcaa cttgcctgcg gggccggatc gccttataat aacggccaat
     2041 tttcccccgg atggaccgcc atccgtcccg cgcgaaggag ccgccgatgc cgacgaccga
     2101 agacctgatt atgatgttgc gggtgctgac cgctttcctg ctcggcgggc tggtcggctg
     2161 ggagcgcgaa cgcatgcaac gccccgcggg cctgcgtacg catatgctgg tggcggccgg
     2221 cgcggcctgc tttaccgtcg ccagtattta cggcttcgcg ggcgagggca ccaaccgcga
     2281 cccggcgcgc ctggccgccc agatcgtcag cggtatcggc ttcctgggtg cgggcgtcat
     2341 ctttcgcacc ggcggatcgg tccgcggcct gaccacggcg gcctcgatct ggatagtggc
     2401 cgcgctgggc gtgctggccg gacagggcct ctactgggtc gcctcgtttg ccacggtgct
     2461 cacctggttc gccctgcgcg tgttgaagag cgcggaacgc ttcggcggcg acccggacag
     2521 cgcgcctgac ggcgaggagg atcgcgacga cggcccgccc gcggcacccg ccggggaaag
     2581 cacggaaccg cgctgacggg gcggggatac ggatgcaaca cggggggacc gagccatgct
     2641 gacgagaggg cgcgcactga tcacggcgca cccgcgcgcc gtgctagtcg cgctgctgct
     2701 ggcggggctg ctggtgcgcc tgccattcgc cggcatcgat acccacctga gcaacgatat
     2761 tatcgtcttt acgcagtggg cgcggctgct ggaactgcgc ggcctcgccg ccatctacca
     2821 ggacccctcc atcaactacc cgccgatccc tctctatctg ctgggcggtg ccgccctgct
     2881 cgacacccat ctgttccgcg gcaacaccac ggcgctcgtc gcgctgatca agctaccggg
     2941 catcctctgc gatgtcgcca ccgccgcact gctggcctgg acggtccagc ggcggggccg
     3001 gcgcgtcagc ctgggcgtct gcgccctgta tctgttcaat ccgggggtgt ggtacgtctc
     3061 ggccctgtgg gggcaattcg aggccgtcta tacgctgccg atcgtcgccg gcgtggtggc
     3121 gctggagcgt gggcgcctgg tgcccgcgtg ggtggccgcc gcgctggcgc tggcgaccaa
     3181 gccgcagagc ctggccctgg cgccggtgct ggtgggcgca tcgctggccc ggcgggggct
     3241 acgcggcctc gtgcttggcg cgggcgccgc gctgctggtg gcgttcgggc tcgccctgcc
     3301 ctggctcggg gccggcaacc tgctcgacgc cgccggcgcc tacaccaatg tgcgggccag
     3361 cccctggctc gacgtgagc
//
LOCUS       DASUYC010000627         9215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1856536, whole genome shotgun sequence.
ACCESSION   DASUYC010000627 DASUYC010000000
VERSION     DASUYC010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 9215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9215
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1856536"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2039)
                     /locus_tag="VF276_10205"
     CDS             complement(<1..2039)
                     /locus_tag="VF276_10205"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HEX6607275.1"
                     /translation="MSTHPRQYPRVARNILLVVALLGLVLLATTPPPARATVYNIADG
                     DVAALLTAIQNVHGNSEITVNLAPNGSYTFDQQVGATTLSTIINASLTINGNGATIAR
                     SSASGTPEFNFFSIGEGTLTLKDLTLRNGSGTNGGAIESGACNLRLINVTFNGNHAVG
                     NGGALALNSCTVDMTTVTFSDNHATGNGGALALDVNSGTTIRDSSFLNNSAGGDGGGI
                     YNEGQLSVDNGTFRDNTAQNGGAITLDEQVDNTASITNSSFSTNTATNGGALLLRGPN
                     TTAVGNSVLNGNHALYGGGIYGTGIVMSVDRSLIISNTAEFQGGGIYVPGRLTVQYST
                     VAANIAGTGPPAAGQQGGGIYAAAGAVLACTSCTIRDNQAGDQGGGLYLARLQLDSEM
                     TGYGITASTISGNRAVTEGGGVYLTGTNYRPGPGVPTHVTFEQSTISGNTAATGGGLY
                     VRGGPLGTDEELVRTFLYFTTIAANQATTAGGGFHNGGLLMLTQPTEGTTWQGGNSLI
                     AGNSAPTGPDIDGTVTSGGYNLIGNSSGGSGFVAADLLDVDPHIGPLLYNGGPTLTHA
                     LLPGSPAINAGAPGQCSAQDQRGLPRTDGACDIGAYEVGNTSRGTAQVLSGPTTDSAA
                     FLGDSNWFKIPVSDPDSVITVTLNSPGDYDLYLFAPKVDEETGQLRDIGQLNTIGA"
     gene            2307..6386
                     /locus_tag="VF276_10210"
     CDS             2307..6386
                     /locus_tag="VF276_10210"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6607276.1"
                     /translation="MDAAAPYIPMDRRQALAAGRELPPTAAGAAIFADISGFTPLTEA
                     LARELGPQRGAEELTRQLNLIYSRLIDEVDRYRGSVIGFAGDAITCWFDADDGRRAVA
                     CALAMQQVVRQIGALTTPASRAVTLAIKVGVAAGPARRFEAGDPLVQRIDVLAGATVF
                     RMAEAEQQAEKGEIVAGAEVVANLGDLLTVREWRQARDGGPRFAVVAGLNGPVAADPW
                     APIAPGILTEEQVRPWLLAPVYRRLLAGQGQFLAELRPAVALFLRFAGLDYDADETVA
                     HKLDAFVRWVQQVVNRYDGALLKLAMGDKGSYLHAAFGAPVAHDDDASRAVAAALELR
                     ALPTELAYITTVQIGISQGRMRVGAYGSPSAHTYDMMGDHANLAARLMSGAAPGQIMI
                     SPAIADVVAQLYELEFIGPLSVKGKSRPVPVSLVQGRKATSPQRSAGFFAGPLVGRDA
                     EIGQIMQVVAAVAAGTGRILRLEGPTGVGKSHLAAALTDRAAAQGFQVAVGACQSTTE
                     HTAYAPWRQVLNALLDPPAGPGAGPQETPARRVAHLEQMVGGIDPTLLLRLPLLGDLL
                     ELPIPDNATTAAFEPRLRQRALVELIVELLRAWAQARPLLLLLDDVHWLDEASQGLAV
                     ALARALAPARVLLVVTHRPPPPGQAILPELPDSPLHTRLNLAELSPAAVAGLIANRLG
                     GPADSLLTALIQSETQGNPFFVEEMLDALRESARIAPGADGRWTLAAPMLEALRHANS
                     LVRQDGGWVLAPTTPLPALQLGLPDSIQGAVLSRLDRLPEAAKLTLKVASVIGRIFGL
                     ALLARSHPLQPPAAALGEQIAVMEERDFARLEVPPPDTAYMFKHNITRDVAYETLLFD
                     QRRQLHQAISRALAALAPAAIDQLAYHSYLGEDWEQALAYLMEAGSRAQKLFANHEAI
                     DHFEKALRCAEKLPADTTPAQRQLIHGNLGRLLTATGQYEPALAHLHTARTLAEQQGD
                     REAQARACRWIARLYENRAEYAAALDWIRQGLTVLGDQQSPARAEMLAIAGLISMRQG
                     NYEQAIAQGEEAMQVAERLGDKGALAFACNSRAVISVIQANNREAVHYFEKTRALYEE
                     TENIYGQAMAENGLANAYFNFGDWGRATAYYRHAGRVFNQTGDIYTLAFVQNNLGGIA
                     LNQGRLDDALADYQRSLQILEQIGGSPYVRGALHMNLGATFVRRGEIAEARAHLELSR
                     AFYAQADARDFLPELHRHFAAAALRGADYDRAVDHANQALALARELAMRVEEATSLHL
                     LGEIALARDDAAGAGSYLTQALPILEDADQEYELARCRITAARLYLATGNPAAAQALW
                     AAAVATFRKLDARLDLQAAAALFPAATVASPSLLPQQQG"
     gene            6597..7451
                     /locus_tag="VF276_10215"
     CDS             6597..7451
                     /locus_tag="VF276_10215"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX6607277.1"
                     /translation="MRDLLYKLIGATGERRDAQLRTWLVLTGGSALATAAVLPYALQM
                     SASRIEKARQAALRAGKPLRAQPPLPVLMGLGVVQSTILFGLVAALGLRTGRAQGLGA
                     PHLEALLNGEPAGLDPQDVAAYAAAGAAGAGGIILLEYTLFHDLHEQLNDGGMGDPGG
                     WKGLLASTYGAIAEEVMMRLGLQTFLSAGIRRLRGETGRPVHPATMWSAVVLANVGFG
                     LGHLPAMVGSVPITGRLALRAVVLNGLVGLILGYLYWRHGLEAAITAHGSADLVLHVL
                     LPAIDRRS"
     gene            complement(7522..8052)
                     /locus_tag="VF276_10220"
     CDS             complement(7522..8052)
                     /locus_tag="VF276_10220"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX6607278.1"
                     /translation="MEIAVLDTEVARIPEGRVASSLDAALAEPLIPGMETSEAVICLN
                     DLEGMDFLACAPSGHELILDTDPAAGGHERGIHPKEMLLVALAGCTGMDVIAILRKKR
                     QVVTDYRVRVAATQRADHPRVYTQIVVEHIVSGAQIDPAAVARAIELSVTRYCSVSAM
                     LSKACPIRHQYRIVPQ"
     gene            complement(8235..>9215)
                     /locus_tag="VF276_10225"
     CDS             complement(8235..>9215)
                     /locus_tag="VF276_10225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013156717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HEX6607279.1"
                     /translation="DPRALSRPLRRLLRPRVRLQIGAATGLDTTQQEVTLAGGQRVAF
                     DYLVLATGARVTPEDVPGFTAGAHHFYTEEAAFRLHAALEEFQGGRIVVGVGGLPHKC
                     PVAPLEFTFLLDEYLTDRGLRGLSEIVYTYPINRVFSIPSVAAAAQPLLEARGIGIET
                     FFNLEAVVPDAKLARSMEGTDLPYELLVMTPPHRGAAFLEGHAIADAQGWVKTDRTTL
                     QVAGTTNLWALGDATDLPISKAGSTAHFEAPVIVEHVTAALRGTTPDPKHAAYGGHVM
                     CFLETGHGKASLLDFDYQRPPKVAAPTAIVHYQKMAFNKAYWYLVPTGVV"
BASE COUNT         1415 a         3036 c         3216 g         1548 t
ORIGIN      
        1 gcgccgatgg tgttgagctg gccgatgtcg cgcaactggc cggtttcctc gtctaccttc
       61 ggcgcgaaga gatagaggtc atagtcgccc ggcgagttga gggtgacggt gatcaccgag
      121 tcggggtcgc tgacggggat cttgaaccag ttgctgtcgc cgaggaaggc ggcggaatcg
      181 gtggtcggtc cgctcaggac ttgggcggtg ccacgcgagg tattgccgac ctcgtaggcg
      241 ccgatgtcgc acgcgccgtc ggtgcgcggc aggccgcgct ggtcctgagc ggaacactgg
      301 ccgggcgccc ccgcgttgat ggcgggggat ccgggcagca gggcgtgggt cagggtgggg
      361 ccgccgttgt agagcaacgg cccgatgtgc gggtccacgt cgagcaggtc ggcggcgaca
      421 aagccgctgc cgccgctgct gttgccgatc aggttatagc cgccgctggt cacggtgccg
      481 tcgatatcgg ggccggtcgg ggcgctgttg ccggcgatga ggctgttgcc gccctgccag
      541 gtcgtgcctt cggtcggttg cgtcagcatg agcaggccgc cgttatggaa gcccccgccc
      601 gctgtggtcg cctggttggc ggcgatcgta gtgaagtata ggaaggttcg caccagttct
      661 tcgtcggtgc ccagcgggcc gccccgcacg tagaggccgc cccctgtcgc ggcggtgttg
      721 ccgctgatcg tgctctgctc gaatgtcaca tgggtcggga cgccggggcc cggccggtaa
      781 ttggtgccgg tgaggtagac cccgccgccc tcggtcacgg cccgattacc gctgatggtc
      841 gacgcggtga tgccgtagcc cgtcatctca ctgtcgagtt gcaggcgtgc caggtagagg
      901 ccgccgccct gatcgcccgc ctggttatcg cggattgtac acgacgtgca ggccagaacg
      961 gcaccggcgg ctgcgtagat ccccccaccc tgctgcccgg cggcgggggg accggtgccg
     1021 gcaatattgg cggccaccgt gctgtactgc actgtgaggc ggccgggcac gtagatgccg
     1081 ccgccctgga actcggccgt gttgctgatg atcaagctcc ggtccacgct catcacgatc
     1141 ccggtgccat agatgccccc gccatagagg gcgtggttgc cgtttaggac gctgttgccc
     1201 acggcggtgg tgttgggtcc gcgcagcagc agcgcgccgc cgttggtggc ggtgtttgtg
     1261 ctgaagctgc tgttggtgat cgaggccgtg ttgtcgactt gctcatccag agtgatcgcg
     1321 ccgccgttct gagcggtgtt gtcgcggaag gtgccgttat ctaccgacaa ctggccctcg
     1381 ttgtagatac cgccgccatc gccgccggcg ctgttgttca gaaaggagga atctcggatc
     1441 gtggtgccgc tgttcacgtc cagggcaagc gcgcccccgt tccccgtggc gtgattgtcg
     1501 ctgaaggtga cggtggtcat atccacggtg caggagttga gggccagtgc gccgccgttg
     1561 ccgaccgcgt ggttgccgtt gaacgtcacg ttgatcaggc gcaggttaca ggcgccggac
     1621 tcgatggctc ccccgttcgt cccgctgccg ttacgcagtg tcaggtcctt cagagtcagc
     1681 gtgccctcgc caatcgagaa gaagttgaac tccggagtac cggacgcgct gctgcgggcg
     1741 atagtcgcgc cgttgccgtt gatggtcagg ctcgcgttga tgatcgtgct gagcgtggtc
     1801 gcaccgacct gctgatcgaa ggtgtagctg ccgttggggg ccaggttgac ggtaatctcg
     1861 ctgttgccgt gtacgttctg gatcgccgtc agcagggcgg ccacgtcgcc gtcggcaata
     1921 ttatagacgg tagcgcgggc ggggggcggc gtggtagcaa ggagcacgag gccgagcaac
     1981 gcgacgacga gcaggatgtt ccgcgcaaca cgaggatatt gccggggatg ggtggacatg
     2041 gggacctctt tcagactacg cagccctggg tgtggcgatg ggccggtctg ccgcgtcgcg
     2101 ccacctgctg ccttcggcaa aggcgacgta tgaccggtcc tgagtcctgg gttgatcttg
     2161 agtggagaaa ggccgcaacg gtggtcctgg cgatcctggc tgttggtacc caggaggtat
     2221 tatactacaa aatggcggcc cgatcatgag tgctgaaccg cgcgatctag cgtgtgctat
     2281 aatgagcctg aaagaaggag accacgatgg acgcggcagc tccgtacatt cccatggacc
     2341 gccgccaggc gctggcggcc gggcgggagc taccccccac ggcggcgggc gcggcgatct
     2401 ttgccgacat ctccggcttc accccgctca ccgaagccct ggcgcgcgaa ctcggcccgc
     2461 agcgcggcgc ggaagaattg acgcgccaac tgaacctgat ctacagccgg ctcatcgatg
     2521 aggtggaccg ctatcgcggc agcgtgatcg gctttgccgg ggacgcgatc acctgctggt
     2581 tcgacgccga cgatggccgg cgggcggttg cctgtgcgct ggcgatgcaa caggtcgtgc
     2641 ggcagatcgg ggcactgacc actccggcga gccgcgccgt taccctggcg atcaaggtag
     2701 gggtggcggc cggcccggcg cgccgcttcg aggccggcga tccgctggtg cagcgaattg
     2761 acgtcctggc cggggccacc gtctttcgca tggccgaggc cgagcagcaa gccgagaaag
     2821 gcgagatcgt cgccggcgcg gaggtcgtgg ccaacctggg cgacctgctc accgtgcgcg
     2881 agtggcggca ggcgcgggac ggcgggccgc gcttcgcggt ggtcgccggg ttgaacggcc
     2941 cggtggctgc cgacccctgg gcgcccatcg caccgggcat attgaccgag gaacaggtgc
     3001 gcccctggct gctggcaccg gtctaccggc ggctgctggc cgggcagggg caattcctgg
     3061 ccgaactgcg cccggcggtg gcgcttttcc tgcgctttgc cggcctcgac tatgatgccg
     3121 acgagacggt ggcgcacaag ctcgatgctt tcgtccgctg ggtgcagcag gtcgtcaacc
     3181 gctatgacgg cgcgctgctc aaactcgcca tgggcgacaa ggggagctat ctccacgccg
     3241 ccttcggcgc gccggtggcc cacgacgacg acgccagccg cgccgtggcg gcggccctcg
     3301 aactgcgcgc tctcccgacc gaattggcct atatcaccac cgtgcagatc ggcatcagcc
     3361 aggggcgcat gcgggtgggg gcctatggca gcccgagcgc gcacacctac gatatgatgg
     3421 gcgaccacgc caacctggcc gcccgcctga tgagcggcgc cgcgcccggc cagatcatga
     3481 tcagcccggc catcgccgac gtggtggccc aactctacga actggagttc atcggcccgc
     3541 tgagtgtgaa gggcaagagc cggccggtac cggtgtcgct ggtgcagggc cgcaaggcga
     3601 cctcgccgca gcgctcggcc ggtttcttcg ctgggccgct ggtcgggcgc gatgcggaga
     3661 tcggccagat aatgcaggtg gtggcggcag tcgccgctgg caccgggcgt atcctgcggc
     3721 tggaaggccc gaccggcgtg ggcaagagcc acctggctgc cgcgctgacc gaccgggctg
     3781 ccgcgcaggg gttccaggtc gcggtcggcg cctgccagag caccaccgaa cacaccgcct
     3841 acgccccctg gcggcaggtg ttgaacgcgc tgctcgaccc ccccgccggg cccggtgccg
     3901 gcccgcaaga gacgccggcg cggcgcgtgg cccacctgga gcaaatggtc ggcgggatcg
     3961 atcccacttt gctgctgcgc ctgcctttgc tcggtgatct tctggaactg cccatcccag
     4021 acaacgccac gaccgccgcc ttcgagccgc gcctgcgcca gcgcgccctg gttgagttga
     4081 tcgtcgagct actgcgcgcc tgggcgcaag cccgcccgct gttgctgctg ctggacgatg
     4141 tgcattggct ggacgaggca tcgcaggggc tggcggtggc cttggcgcgg gcactcgcgc
     4201 ccgcgcgggt gctgctggtc gtgacccacc gccccccgcc gccgggccag gcgatcctgc
     4261 ccgaactgcc ggacagcccg ttacacaccc ggctcaacct ggccgaacta tcccctgcgg
     4321 cggtcgcggg cctgatcgcc aatcggctgg gtgggccggc cgattccttg ctgacggcac
     4381 tcatccagtc tgaaacgcaa ggaaatccct tctttgtcga agagatgctc gacgcgttgc
     4441 gcgaatcggc gcgtattgca ccgggcgcgg atggccgctg gaccctggcg gcgcccatgc
     4501 ttgaggcgct acgccacgcc aacagcctgg tccggcagga cggcggatgg gtgctcgcgc
     4561 ccaccacccc gctcccggcg ctccaactcg ggctacccga ttcgatccag ggggcggtgt
     4621 tgagccgcct cgaccgattg cccgaagcgg cgaagctgac gctgaaggtc gccagcgtca
     4681 tcgggcggat cttcgggctg gcgctgctgg cccggtccca cccactgcaa ccgcccgccg
     4741 ccgccctggg cgagcagatc gctgtgatgg aggagcgcga ttttgcacgc ctggaggtgc
     4801 cgccgcccga caccgcctac atgttcaagc acaacattac ccgcgacgtg gcctacgaaa
     4861 cgctcctctt tgatcagcgg cggcaactgc accaggcgat cagccgggcg ctggcggcgc
     4921 tggccccggc ggctattgac cagcttgcct atcacagcta tctgggcgag gattgggagc
     4981 aggcattggc ctacctgatg gaggccggga gccgggcgca aaagctgttc gccaaccacg
     5041 aggcgattga ccacttcgag aaggcgttgc gttgcgccga gaagctgccc gcggacacta
     5101 cccccgccca gcggcaactg atccacggca acctgggccg gctcctcacc gcgaccgggc
     5161 agtatgagcc cgcgctggcg catctgcaca ccgcgcggac actggccgag caacaggggg
     5221 accgggaagc ccaggcccgc gcctgccgct ggatcgcccg cctgtacgag aaccgggcgg
     5281 aatacgccgc cgcgctggat tggatccggc agggtctgac cgtgctgggc gaccagcaga
     5341 gtccggcgcg agccgagatg ctggcgattg ccgggctgat cagcatgcgc cagggcaact
     5401 acgaacaggc tatcgcgcaa ggggaggagg cgatgcaggt ggcggagcgg ctgggcgaca
     5461 agggggccct ggccttcgcg tgcaacagtc gggccgtcat ctcggtcatc caggccaaca
     5521 accgcgaagc cgtccactac tttgaaaaga cgcgcgccct ctacgaggag accgagaaca
     5581 tttacggcca ggcgatggcg gaaaacggcc tcgccaacgc ctattttaac ttcggcgatt
     5641 ggggtcgcgc cacggcgtac tatcggcacg ccggccgcgt cttcaaccag acgggcgaca
     5701 tctatacgct cgcctttgtc cagaacaacc tgggcgggat cgcgctgaac cagggccgcc
     5761 tggacgacgc cctggccgac taccagcggt cgctgcaaat tctggagcaa atcggcggct
     5821 ccccctatgt gcgcggggcc ttacacatga acctgggcgc gactttcgtg cggcgggggg
     5881 agattgccga ggcgcgcgcc cacctggagt tgagccgcgc cttttacgcg caggccgatg
     5941 cccgcgattt cctgcccgaa ctgcatcgcc atttcgccgc agcggccttg cgcggggccg
     6001 actacgatcg ggccgttgat catgcgaacc aggccctggc gttggcgcgg gagttagcga
     6061 tgcgcgtgga agaggcgacc agcttgcacc tgctgggcga aatcgcgctg gcacgggacg
     6121 acgccgcggg agccggctcc tacctgacgc aggcgttgcc catcctggaa gacgccgacc
     6181 aggagtatga actggcacgc tgccggatca ctgcggcccg gctgtacctc gccacgggca
     6241 atcccgccgc cgcccaggcg ctctgggccg ccgcggtggc aacattccgc aagttggatg
     6301 cccggctcga tctccaggcc gccgccgccc tcttccctgc tgcgacggtg gcttcgccat
     6361 ccttgcttcc gcaacagcag ggctgaaagc gcccgaccgg ttgctgctgc cgagagaagg
     6421 gcagctacgc tgcgcgccgg caagcggtgc cggcataacg catccgcgct ccgcggtgtt
     6481 agctaaagcg tgatatggct gccacgcggc ggtcgcgact ccgcactagg cgtaggggct
     6541 caccggatga gaaccgccca agagcgcgtg ccggacagga ggacaggtga gaagctatgc
     6601 gagatctgct ctacaaattg attggcgcca cgggtgaacg gcgggatgcg caactccgca
     6661 cctggctggt gctgacgggg ggcagcgccc tggccaccgc cgctgtgctg ccttacgcct
     6721 tgcaaatgag cgcttccagg atcgagaaag cgcggcaggc cgcgctgcgg gccggcaaac
     6781 cactgcgtgc gcagcctccg ctgccggtac tcatggggtt gggcgtggtg cagagcacga
     6841 tcctgttcgg cctcgtcgcc gcgctcggtt tgcgaaccgg tcgcgcacag gggctggggg
     6901 cgccacacct ggaagcgctg ctcaacggcg agcctgccgg gcttgatccg caagacgtag
     6961 ccgcttatgc tgccgcgggc gccgccggcg cgggaggcat catcttactc gaatacaccc
     7021 tcttccacga cctacacgag caattgaacg acgggggcat gggcgatccc ggcggctgga
     7081 aggggttgct ggcgagcaca tatggcgcaa ttgcggagga ggtgatgatg cggctggggc
     7141 tgcagacgtt cctctcggct ggtatccgtc ggttgcgcgg cgagacgggc cgcccggtgc
     7201 acccggcgac gatgtggagc gccgtcgtgc tagctaacgt cggtttcggg ctgggccatc
     7261 tgccggcgat ggtgggcagc gtgccgatca ccgggcgcct ggcgctgcgg gcggttgtgc
     7321 tgaacgggct ggtcggcttg atcctgggct atctctactg gcggcacggc ctggaggcgg
     7381 cgatcaccgc ccacggctcc gccgacctgg tgctgcacgt gctgctaccg gccatcgatc
     7441 ggcggagtta ggcgggtcga aaccgcgcca ggtgttcctc gccccggctg ctccggtcgt
     7501 taggtgtcgc ccggccgccg ctcactgcgg cacaatgcgg tactggtgcc ggattgggca
     7561 cgccttgctg agcatggcgc tcaccgagca atagcgggtc accgatagct cgatcgcgcg
     7621 cgccaccgcc gccgggtcga tctgtgctcc actgacgata tgctcaacga cgatctgcgt
     7681 gtaaaccctg gggtggtccg cacgctgggt cgccgccacg cgcacccggt agtcggtgac
     7741 cacttggcgc ttcttgcgca agatggcaat cacatccatc ccggtgcagc ccgccagcgc
     7801 gaccagcaac atctccttcg gatgaatccc gcgctcgtgc ccgccggcgg ccggatccgt
     7861 atcaaggatc agttcgtgcc ccgacggagc gcaggcgaga aagtccatgc cctctaggtc
     7921 gttcaaacaa atcacggcct cgctggtttc cataccgggg atgagcggtt ctgccagcgc
     7981 cgcgtcgagc gaagacgcga cccgcccctc gggtatgcgc gcaacctcgg tatccaaaac
     8041 ggcgatttcc acttgctcct ccacctttca ttgcgtgggc accgcctgcc cggtgcctgc
     8101 ttgtagcggc cccgccgccg tcgaccagat gcataccggt cggggggtta catccgctca
     8161 tcctttcctg tgcccggcac cggcgcagcg cccacgcacc gcgcggcgct ctacgccggc
     8221 gctgggcacg ggggttacac caccccggtg ggtaccagat accagtaagc cttattgaag
     8281 gccattttct ggtaatgcac aatagcggtc ggcgcggcta ccttcggcgg acgctggtag
     8341 tcaaagtcca gcaggctggc tttgccatgt cctgtctcca ggaagcacat tacgtggccg
     8401 ccatatgcgg cgtgtttggg atccggggtg gtgccccgca atgccgccgt gacgtgctcc
     8461 acgatgaccg gcgcctcaaa gtgcgcggtg ctgccggcct tgctgatcgg caggtcggtc
     8521 gcgtcgccca gcgcccagag gttggtcgtg cctgccacct gcagcgtcgt ccggtcggtc
     8581 ttcacccagc cctgtgcatc ggcgatcgcg tggccttcca ggaacgcggc gccccggtgg
     8641 ggcggggtca tcaccagcag ttcgtagggc aggtcggtgc cctccatgct gcgggccagc
     8701 ttcgcgtccg gcaccactgc ttccaggttg aagaaggttt cgatgccgat cccgcgagct
     8761 tccagcagcg gttgggccgc cgccgccacg ctggggatgc tgaacacccg gttgatcgga
     8821 taggtataga cgatttcgct gaggccgcgc agcccgcggt cggtcaaata ctcatcgagc
     8881 aggaaggtga actccagcgg cgccaccggg catttgtgcg gcagcccgcc cacgccgacc
     8941 acaatgcggc cgccctgaaa ttcttcgagg gccgcgtgca ggcggaaggc ggcctcttcc
     9001 gtgtagaagt ggtgcgcgcc agccgtgaag ccgggcacgt cctcgggggt cacccgcgcg
     9061 ccggtggcca gcaccaggta atcaaaggcg acgcgctggc cgccggccag ggtaacttcc
     9121 tgctgggtcg tatccaggcc ggtcgccgcc ccgatttgta agcgcacgcg ggggcgcagc
     9181 aggcggcgca ggggccggct cagggcgcgc gggtc
//
LOCUS       DASUYC010000628         5011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1470428, whole genome shotgun sequence.
ACCESSION   DASUYC010000628 DASUYC010000000
VERSION     DASUYC010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5011
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1470428"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VF276_10230"
     CDS             <1..327
                     /locus_tag="VF276_10230"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX6607280.1"
                     /translation="AQQRPDVYGLVGSYSGATRYDDYRVERELASAPPRAARFHLVAG
                     LYERCLNHPHGAPGGPWDLLDNQRRLAAVLARRGIAHAAHEYPDGHNWGFWADHLPEG
                     LAWLFA"
     gene            complement(420..1883)
                     /gene="obgE"
                     /locus_tag="VF276_10235"
     CDS             complement(420..1883)
                     /gene="obgE"
                     /locus_tag="VF276_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX6607281.1"
                     /translation="MFYDKAKIYVKAGKGGDGSASFRRERYIPNGGPDGGDGGRGGSV
                     ILRVNPELNTLVPFYHKQHFDAPNGGNGRAKKQHGANGADLFIDVPPGTVVQVVEEDI
                     IDPTVTSMEQFDLTEPGQQLVVAKGGRGGLGNVHFATPVHQAPRIAENGEPGQERTLL
                     LELKLIADVGLVGYPNAGKSTLLSQISAAKPKIADYPFTTLEPQLGVVAVDDEAFVVA
                     DIPGLIEGASSGAGLGLEFLRHVERCRLLIHLIDGASGLYPGVSEEDLAALEGADLPE
                     RNPLADFQRINHELAEYSAPLATRPQIVVINKMDLPEVQQRWPKLRTWFTTHGYPVLA
                     ISAATGQGVPELVRRTAAELRELPPREALAPAPPAPVREDEEAVTHRLRPTLDENDFT
                     VTKVEDGFYRVFGTRIERLVNMTRMDNEESLDRLQNILEKSGISRALTEAGVAPGDTV
                     VIGRAELQWTDEPWLTPEGRRAARRGSRHEGPGKQHS"
     gene            complement(1964..2428)
                     /locus_tag="VF276_10240"
     CDS             complement(1964..2428)
                     /locus_tag="VF276_10240"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX6607282.1"
                     /translation="MSDEQYAIHTAHGEEEHSAGGVPVRRGADGGWEVLCLERPRYGD
                     WSLPKGHLEPGETAEAAALRELEEETGVRGQIVRFIRTIRYPITGRSGRPTTKVVDHF
                     LIAVDPDSGPAAPQPPELDIPHWLPLATAAATLTHESERASIQAAAALLAPE"
     gene            complement(2425..3072)
                     /locus_tag="VF276_10245"
     CDS             complement(2425..3072)
                     /locus_tag="VF276_10245"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX6607283.1"
                     /translation="MLHTVIFDLDGTLIDTMDLIVASWQHCTEVHCGAPIPREAVLPT
                     IGLPLVPALEAYAPGKGELLYATYQEHNHAWHDRLAHLVPGTREMLEQLQTAGVALGL
                     VSSKRHGILEMGLNLHKLGPYFGAVIGLEDTARHKPFPDPLLCALEHLGRAPHPDHVA
                     YVGDAASDLAAAQAAGVRPIGVPWGATAAAELRAAGANPLLAGWDDLIALAEAPA"
     gene            complement(3191..3907)
                     /gene="rpoD"
                     /locus_tag="VF276_10250"
                     /pseudo
     CDS             complement(3191..3907)
                     /gene="rpoD"
                     /locus_tag="VF276_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913240.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
     gene            complement(4355..4894)
                     /locus_tag="VF276_10255"
                     /pseudo
     CDS             complement(4355..4894)
                     /locus_tag="VF276_10255"
                     /inference="COORDINATES: protein motif:HMM:NF015912.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
BASE COUNT          694 a         1661 c         1787 g          869 t
ORIGIN      
        1 gcccagcagc ggcccgatgt atacgggtta gtaggcagct attcgggcgc cacacgctac
       61 gacgattacc gggtcgagcg cgaactcgcc tcggccccgc cgcgggccgc gcgcttccac
      121 ctggtcgccg gactgtatga gcgctgcctg aaccacccgc acggcgcgcc cggcggcccc
      181 tgggacctgc tggacaacca gcgccgcctg gccgccgtcc tggcccgccg gggcatcgcc
      241 cacgcggccc acgaataccc cgacggtcac aactggggct tctgggccga tcacctgccg
      301 gaggggctgg cgtggctctt cgcgtagccg tcagccgtca gctatcagcc gtcaggagtt
      361 aggagcagaa gtatccgtag attactgaga actgacggct gacggctgat agctgacggc
      421 tagctgtgct gcttgccggg gccttcgtgg cggctgccgc ggcgggcggc gcggcggcct
      481 tcgggcgtca gccagggctc gtcggtccat tgcagttcgg cgcggccaat gacgacggta
      541 tcgcccggcg ctaccccggc ctcggtcagc gcccggctga tgccgctttt ctccaggatg
      601 ttttgcagcc ggtccagcga ctcctcgttg tccatgcgcg tcatgttgac caggcgctcg
      661 atgcgtgtgc cgaacactcg gtagaagcca tcctcgacct tcgtgaccgt gaagtcgttt
      721 tcgtcgaggg tcgggcgcag gcgatgggtg acggcctcct cgtcctcgcg cacgggcgcg
      781 ggcggcgcgg gcgcgagggc ttcccgcggc ggcagttcgc gcagttcggc ggcggtgcgg
      841 cgcaccagtt cgggcacgcc ctggccggtg gccgccgaga tcgccagcac cgggtagccg
      901 tgcgtggtga accaggtgcg cagcttgggc cagcgctgct ggacctcggg caagtccatc
      961 ttgttgatca ccacgatctg cgggcgggtc gccagcggcg cgctgtactc ggccagttcg
     1021 tgattgatgc gctggaaatc ggcgagggga ttgcgctccg gcagatcggc gccttcgagc
     1081 gcggccaggt cctcttcgct gacgccagga tacaggcccg acgcgccgtc gatcaggtgg
     1141 atcagcaggc ggcagcgctc gacgtggcgc aggaactcca ggcccaaccc ggccccgctg
     1201 ctggcgccct cgatcaggcc ggggatgtcg gccacgacga acgcctcgtc gtccacggcc
     1261 acgacgccca gttgcggctc cagcgtggtg aacgggtagt cggcgatctt gggcttggcg
     1321 gcgctgatct ggctgagcag ggtcgacttc ccggcgttgg gatagcccac caggcccacg
     1381 tcggcgatca gcttgagctc cagcagcagc gtgcgctcct ggcccggctc gccgttctcg
     1441 gcgatgcgcg gcgcctggtg gaccggcgtg gcgaaatgga cgttgcccag cccgccgcgc
     1501 ccgcccttcg ccaccaccag ttgctggccc ggctcggtca ggtcgaactg ctccatgctg
     1561 gtgacggtcg gatcgatgat gtcctcctcg accacctgca cgacggtgcc gggcggcacg
     1621 tcgataaaca ggtcggcgcc gttggcgccg tgctgcttct tggcgcggcc gttgccgccg
     1681 ttgggcgcgt cgaagtgctg cttatgatag aacggcacca gggtgttcaa ctcgggattg
     1741 acccgcagga tgaccgagcc accccgccca ccgtcgccgc cgtcggggcc gccgttgggg
     1801 atgtaacgct cgcgccgaaa ggacgcgctg ccgtcgccgc ccttgcccgc cttcacatag
     1861 attttcgctt tgtcgtaaaa catgatcgcc ccttcattat tttgctctgg ctctggctct
     1921 ggtatgtata tagtatacgt gcggtgagca agttccgggc ctgctattcg ggggcgagca
     1981 gggccgcggc ggcctggatc gaggcgcgct cggactcgtg ggtcagggtg gcggcggcgg
     2041 tggcgagggg cagccagtgg gggatatcga gctccggggg ttggggcgcc gcgggaccac
     2101 tatcggggtc cacggcgatg aggaaatggt ccacgacctt ggtcgtcggg cgcccgctgc
     2161 gcccggtgat ggggtagcgg atggtgcgga tgaagcgcac gatctgcccg cgcacgccgg
     2221 tttcttcctc cagttcgcgc aacgcggcgg cttccgccgt ctcgccgggt tcaaggtggc
     2281 ccttgggcag cgaccaatcg ccgtagcgcg ggcgctccag gcagagcacc tcccagccgc
     2341 cgtccgcccc ccggcgcacg ggcacgcccc cggcggagtg ctcctcctcg ccgtgggccg
     2401 tgtggatggc atactgctcg tcgctcatgc cggggcttcc gccagggcga tcaggtcgtc
     2461 ccagccggcc agcagcggat tggcccccgc ggcccgcaac tcggcggcgg cggtcgcgcc
     2521 ccagggcacg ccgatcgggc gcaccccggc ggcctgagcg gcggccaggt cgctcgccgc
     2581 atcgcccaca taggcgacgt gatcgggatg gggcgcgcgc cccaggtgct ccagggcgca
     2641 gagcagggga tcggggaacg gcttgtgccg ggcggtatcc tccagcccga tcaccgcgcc
     2701 aaagtagggg ccgagcttgt gcaggttcag gcccatctcg aggatgccgt ggcgcttgct
     2761 gctgaccagg cccagggcca ctcctgcggt ctgcaactgc tccagcatct cgcgcgtgcc
     2821 cggcaccagg tgcgccagcc ggtcgtgcca ggcgtggttg tgctcctggt aggtggcgta
     2881 gagcagttcg cccttgccgg gcgcgtaggc ttccagggcg gggaccagcg gcaggccgat
     2941 ggtcggcagc accgcctcgc gcgggatggg cgccccgcag tggacttccg tgcaatgttg
     3001 ccaggacgcc acgatcaggt ccatcgtgtc gatcagcgtg ccgtccaggt cgaagataac
     3061 ggtgtgcagc atcgtagcta tcagccgtca gccatcagct atcaggaacg agctacgcac
     3121 accgcaatgc tgtgttggcg gcccataagg tggacctgat agctgacggc tgacggctga
     3181 tagctgacgg ctactccagg taatccttca gcttcttggc gcgcgacggg tggcgcaggc
     3241 ggcgcagggc cttcgactcg atctggcgga tgcgctcgcg ggtgacgccg aactcgcggc
     3301 ccacctcttc gagcgtgcgg gcgcgcccgt ccttgaggcc gaagcgcagt tgcaacacca
     3361 gccgttcgcg ctcgttcagc gagtcgaggg ccagcgcgac ctgctctttc agcaggcgat
     3421 tggacgcggc gtcggcgggg gccagcgcct tgtggtcctc gatgaaatcg gccaggtgcg
     3481 tgtcctcttc ctcgccgatg ggcgtttcca gcgacaccgg ctcctggctg atcttgatga
     3541 tctcgcgcac gcgctcggcg gacatgttga ggtcctgggc catctcctcg gaggtcggct
     3601 cgtggccgag ttcctgcgac aggcggcgct gggcgcggac gacgcggttg atcgtctcga
     3661 ccatatgcac agggatgcgg atcgtgcgcg cctggtcggc gatggcgcgg gtgatggcct
     3721 ggcgaatcca ccaggtggcg taagtggaga acttgaagcc cttggtgtag cggaacttct
     3781 ccaccgcgcg gatcaggccg atgttgccct cttgcaccag gtcgagcatg gtcatgccgc
     3841 gcccgatgta cttcttggcg acgctgacca ccaggcgcag gttggcctcg gtcaggtggc
     3901 ggcgcgcggc ggtcgaatcg gcgataatat cggtccagta atagcgcagg tgcccggcca
     3961 tcgcggtcag catggcggcc aggtcggtct ccagcggcca ttcccaggca tcgacgcggg
     4021 cgcgcagggg cgcgggcaag agctcaaaca gcagcagcgt gtggcgcagg tcgtcctcca
     4081 actgctgcga gtggtggccc atctggttcg ccacgccctt gagcacggtg ggatcgagtt
     4141 gtgaggtggg gatgatcgcc gtgagcacgt cgtggcgcgt gggcggcagc gagccgccgt
     4201 actgcgcgca gtagacctct tcggccagca tccagccccg gcggagttcg ttgtagacct
     4261 cgcggcagat gagcgtgccg ggcatggtgg cggcgggccc tagccccaac tcgctcaggt
     4321 tggcgacgag ctgctccagg aagatgccgc gctccatgcg cttcgccagg gcgatctcgg
     4381 cgtcggcggt cagcagcggc acgcgcccga tctcgcgcag gtagaggcgc acggggtcgt
     4441 cggtgcccac gccgtcgagg tcggcggtga ggtcgaccat gatcgggctg ccgggaccgg
     4501 gggtctcggt gggcggcgcc gggggccact cggcctcgtc gatgatctcg acgttgaggt
     4561 ccagcaactc ggcgtagatc tcgtccacct cggcgatgtg cgtctcgggg gcgggttgga
     4621 cctggagaat ctggtcggcc gtcacaaagc cctgccggcg ggcacactcc agcagctcgg
     4681 ccacatcgct gcccgcgccg gagtcctcgt cgggccgggc gggcaggtcg cacgcggcga
     4741 ccgtctgccc gtcctccccc agcataaagg tggcccaggg ggggatgtcg tccaactcgg
     4801 cgccgtcgcc ggtcgcgagt agatcccctt cctcgccaga cgggctccca agttccgcgc
     4861 ctggttcttc cgcaggagtg taacgtttga ccatacgaac ctcctctggt gctgctgaat
     4921 ggccccgatg aatggtgttg cgggttatct ccgtgccgat aaccctctcc tcctgtcagc
     4981 gcttgctttg cgccggtgtg gtgcaccggc g
//
LOCUS       DASUYC010000629         2636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1580323, whole genome shotgun sequence.
ACCESSION   DASUYC010000629 DASUYC010000000
VERSION     DASUYC010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1580323"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="VF276_10260"
     CDS             complement(<1..847)
                     /locus_tag="VF276_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX6607284.1"
                     /translation="MFELPVQLDHSAAFADLDRLLVTKLCIPPARASLVPRPRLTARL
                     TEALGRKLILLAAPAGSGKTTALVDWWATQAGAGADLAWLALDDADNDPVRFWTYAIG
                     ALETVYPGIGEPARAMLVAPQSPLLEWVLTPLINALATIPEDLVLVLDDYHLITTPAI
                     HDSLLFLLDHIPPRMHLVLTARHDPPLPLARLRARGELAEFRSPDLRFTGDEAAGLLA
                     AILGAAPAPEHVALLEARTEGWAAGLQLAALALQGRANADEFIQAFTGSHRYIVSYLA
                     EEVLNR"
     gene            1117..2580
                     /locus_tag="VF276_10265"
     CDS             1117..2580
                     /locus_tag="VF276_10265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX6607285.1"
                     /translation="MSKTTAQENQIMTGPRTPAPPAAPAPDRARRKQAAAISSMLAAV
                     LLAAAKLIVGLLTGSLGLLSEALHSGLDLIGTILSFAAIRVSDRPPDRNHPYGHAKAE
                     SLVALFAVFLLTMTSLGILYEAYRKAFGEPEVPEINVFSFGVLIMALAVDVSRSRYLA
                     RVARETNSQALAADAAHFASDLWSSGTVLVSLILIGAGQVLGWPAHWLSVVDAVAGVA
                     VALVIFHAAWGLAGRAVNSLMDEVPRHLVPALEYVAATTPGVLATDSARLRYVGDKAY
                     ADVVVNVPRGLSLEESEAISENVIAQVRGVVPGADVVVHTHPVAPASERATDLARVIA
                     SRLTVGVHHVRAFETPVGLRLDMHMEVPATQTLAEAHATADQVETALRGEIRGLHAVE
                     IHIEPRHDETYLVEPIADEPLRARVVRAAESVAGPGAVRQVEMGLSKLGAVLTIHVGL
                     PGDLPITEAHARTAEIERAVRDAMGTSYRVTVHPEPV"
BASE COUNT          430 a          951 c          879 g          376 t
ORIGIN      
        1 ggcggttcaa cacctcctcc gcaaggtagc tcacgatata gcgatggctg ccggtgaaag
       61 cctggataaa ctcatccgcg ttggcccggc cttgcaaggc cagcgccgcc agttgcagcc
      121 cggccgccca gccctcggtc cgcgcttcga gcagggccac gtgctccggt gccggcgccg
      181 ccccgaggat cgccgccagc aaacccgccg cttcgtcgcc ggtgaagcgc aaatcgggcg
      241 agcggaactc cgccagctcc ccgcgggcgc gcagccgggc cagcggcagg ggcggatcgt
      301 gtcgcgccgt gagcacgaga tgcatgcgcg gcggtatatg gtcgagcaaa aacagcaggc
      361 tatcgtggat ggccggggtc gtgatcaggt gataatcatc gaggaccagg acaagatcct
      421 ccgggatggt cgccagcgcg ttgatcagcg gggtcagcac ccactccagc agcggggact
      481 ggggcgcaac gagcatcgcc cgcgccggct ccccgatgcc ggggtagacc gtctccaggg
      541 cgccgatcgc gtaggtccag aagcgaaccg ggtcgttatc ggcatcgtcg agcgccaacc
      601 aggccagatc ggcgccggct ccggcctgcg tcgcccacca atcgaccagc gcggtcgtct
      661 tgccggagcc ggcgggcgcc gccaggagga tcagtttgcg ccccagcgcc tcggtcagcc
      721 gcgccgtgag ccggggtcgc ggcaccagcg aggcgcgcgc cggggggatg cacagcttcg
      781 ttaccagcaa gcgatccaga tccgcgaagg cggcgctatg atccaactgc acgggtaatt
      841 caaacatcac tcgtccccaa ccggactttg cggcgagata gagacggcag agggccgcta
      901 tagtggggat tatatgccca aagcaccggg cacgcaacgc cccgcgcggt acgcctgcca
      961 tccgtccata ccggcttatc tcacaccgcc aaggacgcaa agaataagac ctatataaag
     1021 aacctgcgcg tccttcgttg cttcgcggtg aacttgtgaa cggatgtgag gcacgcagct
     1081 tgccccggcc agaccggaag gggtagaata ccggatatga gcaagaccac ggcgcaagag
     1141 aaccagatca tgaccggccc gcgaacgccg gcgccgcctg ccgcccccgc gcccgaccgc
     1201 gcccgacgca agcaggcggc cgccatttcc tcgatgctcg cggcggtgct gctggcggcg
     1261 gccaagctca tcgtcggcct gctcaccggc agcctgggct tgctgtcgga ggcgctgcat
     1321 tcgggcctcg acctgatcgg caccatcctg tccttcgccg ccatccgcgt ctcggaccgg
     1381 ccccccgacc gcaaccaccc ctacggccac gccaaggccg agagcctggt ggcgctgttc
     1441 gccgtcttcc tgctgaccat gacctcgctg ggcatcctct acgaagccta ccgcaaagcc
     1501 ttcggcgagc cggaggtgcc ggagatcaac gtcttcagct tcggcgtgct gattatggcc
     1561 ctggccgtcg atgtgagccg gtcacgctac ctggcccgtg tcgcccgcga gaccaacagc
     1621 caggccctgg ccgccgacgc cgcccacttc gccagcgacc tctggagttc gggcaccgtg
     1681 ctggtcagcc tcatcctgat cggcgcgggc caggtactcg gctggccggc gcactggctg
     1741 tcggtagtcg atgcggtggc cggggtggcc gtggctctgg tcatcttcca cgcggcctgg
     1801 gggctggccg ggcgcgccgt caactcgctg atggacgagg tgccgcgcca cctggtgccc
     1861 gccctggagt acgtcgccgc gacgacgccc ggcgtgctcg ccaccgacag cgcgcgcctg
     1921 cgctatgtgg gcgacaaggc ctatgccgat gtggtggtca acgtgccgcg cggcctctcg
     1981 ctggaagagt ccgaggccat ttccgagaac gtcatcgccc aggtgcgcgg cgtggtgccc
     2041 ggcgccgatg tggtcgtcca cacccacccg gtcgccccgg ccagcgagcg cgccactgac
     2101 ctggcgcgcg tcattgcctc gcggctgacc gtgggcgtcc accatgtgcg ggccttcgag
     2161 acgcccgtcg gcctgcggtt ggacatgcac atggaagtgc ccgccaccca gacgctggct
     2221 gaggcccacg ccaccgcgga ccaggtggag accgcgctgc gcggcgagat ccgcggcctg
     2281 catgccgtcg agatccacat cgaaccacgg cacgacgaga cctacctggt ggaacccatc
     2341 gccgacgaac ccctgcgcgc ccgcgtggtc cgcgccgccg agagcgtcgc cgggccgggc
     2401 gccgtgcgcc aggtcgagat gggcctgagc aagctcggcg ccgtgctgac catccacgtc
     2461 ggcctgccgg gcgacctgcc gatcaccgag gcccacgccc gcaccgccga aatcgagcgc
     2521 gccgtgcgcg acgccatggg cacgtcctac cgggtcacgg tccacccgga gccggtgtaa
     2581 gcagcccgcg ctgctgcaag cgccacagca gcaggtggta gtagagcatc ccgcgc
//
LOCUS       DASUYC010000630         2444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_834435,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000630 DASUYC010000000
VERSION     DASUYC010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834435"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /locus_tag="VF276_10270"
     CDS             <1..1013
                     /locus_tag="VF276_10270"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX6607286.1"
                     /translation="GIVIAAGSVALLWATGGPVRRLPHRGQLALAFGAWILEALLLLI
                     AFSLIAAPVALNLPAPGDLLAMLPLQAIQTLAVLVALVALTLTWRVPGFRAVGLISAG
                     PGARYALRDAAVGLVLGPVAVGVFLVAGLIGGWDRITGVAPVPDMIGSLVLGVVFFLF
                     VAVFEEVSTRGCLFALLARVLGIPAAAAISAVTFGLLHGLNPGATPVALLGVGLAGVV
                     FLFALYRTGALWLPIAFHLSWDWAETSLYGYPDSGIPPSAALRLAIDPNAPAWATGGE
                     FGPEASVFVLVALALAAGVIWLYTRGRPGRTILFPAGAPEHQPPYQPAPLPAPGDGPR
                     YI"
     gene            1035..>2444
                     /locus_tag="VF276_10275"
     CDS             1035..>2444
                     /locus_tag="VF276_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607287.1"
                     /translation="MRLNRLLLLALIVLAGGISLSLFARPATAAPARLSVVSARLAPG
                     TSWPVAAPVTLTVQFDQNVLLASWQWVGADGRPVPWVGSTLPAQDLRPQQNTWVGRAA
                     APPVPGTYGIQVIARAANTPGPQTFTLSLRGTVVAGEGPLTRGLVYVHSGNLWLRSPD
                     LRREQAVTYYGHPGNASQPAWSPDGRRIAFIRDRGTIDSGPELWVINPDGTAPRAVST
                     PTPGHSLGYPVFGADGTLYVSESRELTFQGVDLGDTWDVARVDLATGRRTPVLPGALM
                     PDVSPDGKRMVYIQQTINSDGSVEQGLSVADVDGRNDRLLVAASTTATFYAPSWSPDG
                     KTIAFASTALGPPPLRALGLAAPLLHGGIWDLWAVPAAGGTPVMLSPIQEDLPYPRWA
                     PDGGSVLFISPTGIWRVPAAGGAAIHIADYDEHSELTIWAPVERRAPILPGSSRCFTE
                     TGHCLHGIFRTYWEQHGGLV"
BASE COUNT          278 a          928 c          850 g          388 t
ORIGIN      
        1 ccgggatcgt gatcgccgcg ggcagcgtcg cgctgctctg ggccaccggc gggccggtgc
       61 gccggctgcc gcatcgcggg caactggcgc tggcctttgg cgcgtggatc ctggaagcgc
      121 tgctgctgct gatcgctttc agcctgatcg cggcgccggt ggcgctgaac ctgcccgcgc
      181 ccggcgacct cctggcgatg ctgccgttgc aggccatcca gacgctcgcc gtgctggtgg
      241 cgctcgtcgc cctgaccctc acctggcgcg tgccgggctt ccgcgcggtc gggctgatct
      301 cggcggggcc gggggcgcgc tatgccctgc gcgatgccgc cgttggcctc gtgctggggc
      361 cggtggcggt gggcgttttc ctcgtcgccg ggctgatcgg cggctgggat cgcatcacgg
      421 gcgtggcccc ggtgcccgac atgatcggga gcctggtgct gggcgtcgtg tttttcctct
      481 ttgtcgcggt atttgaggag gtgagcacgc gcggctgcct gttcgcgctg ctggcgcggg
      541 tgctgggcat ccccgccgcc gcggcgatca gcgcggtgac gttcgggtta ctgcacggac
      601 tgaaccccgg cgccaccccg gtcgcgctgt tgggtgtggg gttggccggg gtcgtcttcc
      661 tgttcgcgct ctaccgcacc ggcgcgctct ggctgcccat cgctttccat ctaagctggg
      721 attgggccga gacctcgctc tacggctacc ccgacagcgg catcccgccg tcggcggcgc
      781 tgcggctggc gattgacccc aatgcccccg cctgggccac cggcggcgaa ttcggccccg
      841 aagccagtgt cttcgtgctg gtggcgctgg ccctggccgc gggcgtcatc tggctgtaca
      901 cgcgcgggcg accgggccgg accatcctct tcccggcggg cgcgccggaa caccagccgc
      961 cgtaccaacc cgcgccgttg cctgcaccgg gcgacggccc gcgctatatc tgaaccgacc
     1021 tgaaaggacc tttcatgcga ctcaaccggc tcttgctgct cgcgctcatc gtgctcgccg
     1081 gtggcatttc gctctcgctg ttcgcccgtc ccgccacggc cgcgcccgcg cgcctgagcg
     1141 tggtcagcgc ccgcctggca cccggcacat cctggcccgt ggccgcgccc gtcacgctca
     1201 ccgtgcagtt cgaccagaac gtgctgctgg cctcgtggca gtgggtgggg gccgatgggc
     1261 gcccggtgcc gtgggtgggc agcaccctgc ccgcgcagga cctgcgcccg cagcaaaaca
     1321 cctgggtcgg gcgcgcggcg gcgccgccgg tgcccggcac ctacggcatc caggttatcg
     1381 cgcgggcggc caacacaccc ggcccgcaga ccttcaccct gagcctgcgc ggcacggtcg
     1441 tggccggcga agggccgctc acgcgcggcc tggtctacgt ccacagcggc aacctctggc
     1501 tgcgctcgcc cgacctgcgc cgcgagcagg ccgtcaccta ctacggccac cccggcaacg
     1561 cttcgcaacc ggcctggtcg cccgacgggc ggcgcatcgc cttcatccgc gaccggggca
     1621 ccatcgacag cggccccgaa ctctgggtca tcaaccccga cggcaccgcc ccgcgcgccg
     1681 tgagcacgcc cacgcccggc cactcgctcg gctacccggt gttcggcgcc gacggcacgc
     1741 tctacgtctc cgagtcgcgc gaactcacgt tccagggcgt ggacctgggc gatacctggg
     1801 acgtggcgcg ggtggatctg gcgaccgggc ggcgcacccc cgtcctgccc ggcgcgctca
     1861 tgcccgacgt gtcgccggac ggcaagcgca tggtctacat ccagcaaacg atcaactccg
     1921 acggcagcgt ggagcagggc ctgtccgtgg ccgatgtcga tgggcgcaac gaccggctgc
     1981 tggtcgcggc cagcacaacg gccaccttct acgcgccctc ctggtcgccc gacggcaaga
     2041 cgatcgcgtt cgccagcacc gccctggggc cgccaccgtt gcgcgcgctc ggcctggccg
     2101 cgccgctgct gcacggcggt atctgggacc tgtgggccgt ccccgcggcg ggcggcacgc
     2161 cggtgatgct ctcgccgatc caggaagacc tgccctatcc gcgctgggcg ccagacggcg
     2221 gcagcgtgtt gttcatcagc cccaccggca tctggcgcgt gcccgccgcg ggcggtgccg
     2281 ccatccatat cgccgactac gacgagcaca gcgagctgac catctgggcg ccggtcgagc
     2341 gccgggcgcc gattctgccc ggcagttcgc gctgtttcac cgagaccggc cactgcctgc
     2401 acggcatctt ccgcacctac tgggagcagc acggcggcct ggtg
//
LOCUS       DASUYC010000631         2707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1888803, whole genome shotgun sequence.
ACCESSION   DASUYC010000631 DASUYC010000000
VERSION     DASUYC010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1888803"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..1058)
                     /locus_tag="VF276_10280"
     CDS             complement(96..1058)
                     /locus_tag="VF276_10280"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEX6607288.1"
                     /translation="MSGVLTAIGEILIDFLPIQSGGHTTGFAMHAGGAPFNVAVGMAR
                     LGQPAAFAGKVAADLFGRYLRAYAEEHGVDTRFLIPMEAQSTLAFVAMENGEAAYAFY
                     GEGAADTLLTIDDLPPEFFAETRIFHFGSISLLRGSTPDAVLAAAERLKGRALLSFDP
                     NLRPGLVRDEAAYRALLDRAFALADVVKLSSVDLDWLAPGRTAGEVIADLATRGPGLV
                     VVTQGGAGVLAARRDLEWRVPAFPVRVIDTVGAGDAFSAGLLAGLAEHGVYTRAALDA
                     LPEADLLAALRFAGAVAAMTCARAGADPPRRAEVAAFLAARETP"
     gene            complement(1055..1708)
                     /locus_tag="VF276_10285"
     CDS             complement(1055..1708)
                     /locus_tag="VF276_10285"
                     /inference="COORDINATES: protein motif:HMM:NF013116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HEX6607289.1"
                     /translation="MRIYIDSADEAAIAEALASGYVYGVTTNPTILRRAGARAAGVPA
                     LARMALAAGARELHLQVYSDDPAAMAREGRALAELDPQRVVVKIPATAAGYAAAAQLA
                     ADRVRVTLTAVYTVRQAILAASVGAAYIAVYVGRMGDAGLDAQALVATMQRTLDAQRA
                     PVAILGASIRDAAEIEALALAGVAAVTLPPPVLGQLLDSPLTAAAAAAFRADAGAIL"
     gene            complement(1896..2615)
                     /locus_tag="VF276_10290"
     CDS             complement(1896..2615)
                     /locus_tag="VF276_10290"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HEX6607290.1"
                     /translation="MPEPLTIGIVGGMSPQSTITYYQQILRRHEATLHDHAYPRLVIA
                     SVSFQQYIDWQHAGAWDRVAAGLQREFEAVAAVGADFAILATNTMHKVLPQITSPVPV
                     LHILDAVAARAREDGVRTVGLTGTGFTMADGFYAAGLESRGLGVVLPTADEQAAIHRI
                     IYDELIRGEIVPASVAEFTTIGQRLTARGADAVLLACTELELLTRPAPLPVPVLDTTQ
                     IHADAAWEMALGRRPVPAGAP"
BASE COUNT          414 a          982 c          935 g          376 t
ORIGIN      
        1 ggtcgcgtat tgggtgaaga gggccattgt atctacgccg gtgatcgcgt ctagggacat
       61 aaccgggctt ctcccatcgg gtgacgaggc gaacgtcagg gggtctcgcg ggcggccagg
      121 aacgcggcca cttcggcccg ccgcggcgga tcggcgcccg cgcgcgcgca ggtcatcgcg
      181 gctaccgccc cggcaaagcg cagcgcggcc agcagatcgg cctccggcag cgcgtccagc
      241 gccgcccgcg tatagacgcc gtgctcggcc agcccggcca gcagcccggc gctgaaagcg
      301 tcgcccgccc cgaccgtatc gatcacgcgc acggggaagg cgggtacccg ccactccagg
      361 tcgcggcgcg ccgcgagcac ccccgcgccg ccctgcgtca cgacgaccag gcccggcccc
      421 cgcgtcgcca ggtccgcgat gacctcgccc gccgtgcgac caggggccag ccagtccagg
      481 tcgacggaac tcagcttgac cacgtcggcc agggcgaagg cgcgatccag cagcgcccga
      541 taggcggcct cgtcgcgcac cagaccgggg cgcaggttcg ggtcgaacga cagcagggcg
      601 cggcccttaa gccgctcggc ggcggccagg actgcatccg gcgtgctacc gcgcagcaag
      661 ctgatcgagc cgaaatggaa gatgcgcgtc tcggcgaaga actctggcgg cagatcgtcg
      721 atggtcagca gcgtatcggc ggcgccctcg ccgtagaagg cgtaggccgc ctcgccgttc
      781 tccatcgcca cgaaggccag cgtgctttgc gcctccatcg ggatcaggaa gcgcgtgtcc
      841 acgccgtgct cttccgcata agcgcgcaga tagcgcccga agaggtcggc ggcgactttg
      901 ccggcaaacg ccgcgggctg gcccaggcgc gccatgccca ccgccacgtt gaaaggcgcg
      961 cccccggcgt gcatggcgaa gccggtcgtg tgcccgccgc tctggatcgg caagaaatcg
     1021 atcaggatct cgccgatggc cgtaagcact ccgctcataa gatcgctcct gcgtcggctc
     1081 ggaaggcggc ggcggccgcc gccgtcaagg gggagtcgag tagctggccg aggaccggtg
     1141 gcggcaacgt caccgcggcg acgcccgcca gcgccagcgc ctcaatctcc gccgcgtcgc
     1201 ggatgctggc gccgagaatg gcgaccggcg cacgctgcgc gtcgagggta cgctgcatag
     1261 tggcgaccag cgcctgggcg tcgaggcccg cgtcgcccat ccggcccacg tagacggcga
     1321 tataggctgc acccacgctc gccgccagga tcgcctgccg caccgtatac acggcggtga
     1381 gggtgacacg cacccggtcc gctgccagtt gcgcggcggc agcgtacccg gcggcggtgg
     1441 cggggatctt caccaccacc cgttgcgggt ccaactcggc cagggcgcgg ccttcgcgcg
     1501 ccatcgccgc cgggtcgtcg gagtagacct gcaagtgcag ttcgcgcgca ccggcggcga
     1561 gcgccatacg cgccagcgcg ggcacgcccg ctgcccgcgc gcccgcccgg cgcaggatcg
     1621 tcggattcgt cgtcacgccg tagacgtagc cgctcgccag cgcttcggca atcgcggcct
     1681 cgtcggcgct atcgatatag atgcgcaccg ctcgtccttt ccccgccacc cccgcgggcg
     1741 ctaccatcat agcatagccc ttgccgcggg acaatagccg cccccggcaa ctatccatac
     1801 gggacgattg ctcaccgcga aggcgcgaag aacgcgaaga tcagttattt tagttttatt
     1861 cttcgcgtcc ttcgcgcctt cgcggtgaaa atccgtcacg gcgccccggc gggcaccggg
     1921 cgccggccca gggccatctc ccaggcggca tcggcgtgga tctgcgtcgt gtccagcacg
     1981 ggcaccggca gcggcgcggg ccgggtgagc aattcgagtt ccgtgcaggc cagcaacacg
     2041 gcatcggccc cgcgcgccgt caggcgctgg ccaatggtag tgaactcggc caccgaagcc
     2101 gggacgattt caccgcggat caactcatcg tagatgatgc gatggatcgc cgcctgctcg
     2161 tccgccgtgg gcagcaccac gcccaggccg cggctttcga ggcccgccgc atagaagccg
     2221 tcggccatgg taaacccggt gcccgtcagc ccgacggtgc gcaccccgtc ctcccgcgcc
     2281 cgcgcggcca ccgcgtcgag gatgtgcagc accggcacgg ggctggtgat ctgcggcagc
     2341 accttgtgca tggtgttggt cgccaggatg gcgaaatcgg cgcccaccgc ggccaccgcc
     2401 tcgaactcgc gttgcagccc ggcggccacc cggtcccagg cgcccgcgtg ctgccaatcg
     2461 atgtattgct ggaacgagac gctggcgatc accaggcgcg ggtaggcgtg gtcgtgcagc
     2521 gtggcctcgt ggcggcgcag gatctgctgg tagtacgtga tcgtggactg cgggctcatc
     2581 ccgcccacga tgccgatagt cagcggttca ggcatcagca ctcttctcca taggcggcgc
     2641 tgccggctgc ggcgggcggc gccaggtcga tccagtagcg cagcttgcgg ctgcgctggc
     2701 cgggcgc
//
LOCUS       DASUYC010000632         3170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1195861, whole genome shotgun sequence.
ACCESSION   DASUYC010000632 DASUYC010000000
VERSION     DASUYC010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195861"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VF276_10295"
     CDS             complement(<1..464)
                     /locus_tag="VF276_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607291.1"
                     /translation="MNNPPAPDSPTLAPLAVEGAPGARPPPASEGTPVPTRRRPGTLL
                     ARLIDWGGACAALLLMICVLLWPAPVDRAVLPGVTDLSDLVISHWPSALVLKETMAQA
                     HRLPLWNPFYGGGRPLAADPLAALFYPPTQLVNLLDVRDYFFVLLAGHLLLAG"
     gene            complement(873..1955)
                     /locus_tag="VF276_10300"
     CDS             complement(873..1955)
                     /locus_tag="VF276_10300"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEX6607292.1"
                     /translation="MIQYAAILVTAVAIVLFVTPLVRSAATRFGVTDSPNARKVHANP
                     VPLLGGMAIWAGFVLTLLLFDTAPFVQQFGSIIVGATVASLVGLWDDRWGMKPAFKLF
                     GQIVAAGLMVAFGTSVHFLHNDVLNTAATILWVVGITNALNLMDNMDGLAGGVATWAA
                     FFIFLLAMMSGQRLVAPFALALAGACIGFLYYNFKPGHIFMGDTGSLFLGFLLSAVAL
                     KLRFPVLYDPITGVPIDHQAVTWVIPLLVLGLPIFDTTLVSISRLRRGLPITRGGRDH
                     TSHRLVALGATRREAVLILYLVCCLLGVAAITVMNIGVTEAQVLAGLVAVLAVVAFWR
                     LEQVPLIDTNPKVVEPQGRPQFGPRR"
     gene            complement(1977..>3170)
                     /locus_tag="VF276_10305"
     CDS             complement(1977..>3170)
                     /locus_tag="VF276_10305"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6607293.1"
                     /translation="DMFYKQGLAYDNAQRWPNAIGPYEKAIQIAPNEDFYYLFLGRAY
                     MEWSKRTDQFQGSPPPEQLLTRAQSALLTAESLNPLNTDHYANLGRLYMYWGDTLAQN
                     STQAGQAAQKYAQGIAEYEKAHTLSPGNAEIWNELALAYAKGGRIDDALKAIRGSQQM
                     DDRYEQTPFIAAEIERSSGERYEALAQQPGVTQEMSATYTLSATQEAKAAAADYAATV
                     KLAPGQLQDTDFEKRIDFLNRHDALPVLAAGYQEVISSAVKAKQENPANPDEPVQARA
                     ALGYIEWKLNKTADAITQFERAVQLQCNDFFNLQNLGMLYRDTGQDEKALNMLEAALA
                     VADCNPNDPYAKSCDLLRASTDPKADPAKTAVCTPLTGQSDMSSTINSLRSEVQRLKQ
                     KLGRS"
BASE COUNT          520 a         1007 c         1109 g          534 t
ORIGIN      
        1 ccggccagca gcaggtgccc ggcgagcagc acgaagaagt aatcccgcac atccagcagg
       61 ttgaccagtt gcgtcggggg atagaacagc gcggccagcg ggtcggcggc cagcgggcgc
      121 ccgccgccgt agaacgggtt ccagagcggc agccggtgag cctgggccat cgtctctttc
      181 agcaccagcg cgctgggcca gtggctgata accaggtcgc tcaggtccgt gacgcccggc
      241 aagaccgcgc gatccaccgg cgcgggccag agcagcacgc agatcatgag cagcagcgcg
      301 gcacaagcac cgccccagtc gatcagccgg gcgagcaggg taccgggacg gcggcgggta
      361 ggtacgggtg tcccttcaga ggcaggcggc ggtctggcgc ccggcgcgcc ttccaccgcc
      421 aggggcgcca gggtgggcga atcgggggcg ggcggattat tcatagggtg gcatgggccg
      481 caggttcata gccgttatcg gcagcaagcg ctgtgccact ccccggcgac agctttgcgc
      541 aggtagcccg ccacggtgat tttgcgttgt aggggccggt ttgaaaccgg cccgtcgcta
      601 cgctgaaagc aggcagggcg ggccgggcgg caaggcgggt ttgaaaccgg cccctacacc
      661 gccattccgg cctttttatc gtccgtggcg ttttgaaacc ggcccctaca ccgccattcc
      721 ggccttttta tcgtccgtgg cgtgctgtag gcacatgatt ggcgcatggg aatccgtccc
      781 gaatgttgcg ttgtaggggc cggtttcaaa ccggcccgcc gcgcggctac ggtgtagggg
      841 ctgttttgaa tgggaaatgc ggcccggtga gatcagcggc gcgggccgaa ctggggccgg
      901 ccctgcggct caaccacctt ggggttagta tcgatcagcg gcacctgctc cagccgccag
      961 aaggcgacca ccgccagcac cgccaccagc ccggccagca cctgcgcctc ggtcacgccg
     1021 atgttcatga cggtgatggc ggccacgccg agcaggcagc agaccaggta gaggatcagc
     1081 acggcctcgc gccgcgttgc gcccagggcc accagccggt gcgaggtgtg gtcgcgcccg
     1141 ccccgcgtga tgggcagccc gcgccgcagc cgcgagatgc tgaccagcgt ggtatcgaaa
     1201 atgggcaggc cgagcaccag cagcgggatc acccaggtca cggcctggtg gtcgatgggc
     1261 acgccggtga tcgggtcgta gagcaccggg aagcgcagct tcagggccac ggcggacagc
     1321 aagaaaccga gaaagaggct gccggtgtcg cccatgaaga tgtggcccgg cttgaagttg
     1381 tagtagagga acccgatgca ggcgccggcc agggcgagcg cgaagggcgc caccagccgc
     1441 tggccgctca tcatcgccag caggaagatg aaaaaggcgg cccaggtggc gacgccgccc
     1501 gccaggccgt ccatattgtc catcaggttg agcgcgttgg tgatgccgac cacccagagg
     1561 atcgtcgcgg cggtgttcag cacgtcgtta tgcaggaagt ggacgctggt gccgaaggcg
     1621 accatcagcc cggcggccac gatctggccg aagagcttga aggccggctt cataccccag
     1681 cggtcgtccc acaggccgac cagcgaggcc accgtggcgc cgacgatgat gctgccgaac
     1741 tgctgcacga agggcgcggt gtcaaacagc agcagggtca gcacaaagcc cgcccagatc
     1801 gccatgccgc cgagcaacgg caccgggttg gcgtggacct tgcgcgcgtt gggcgagtcg
     1861 gtgacgccga agcgggtggc cgcgctgcgc accagcgggg tcacgaacag cacaatggca
     1921 accgccgtca ccaggatcgc cgcgtactgg atcatgccca agtctccctg gtccggctag
     1981 gaccggccaa gcttctgctt gaggcgctgg acctcagagc gcaggctgtt gattgtgctg
     2041 ctcatatcgc tttgcccggt cagcggcgtg cagacggccg tcttggccgg atcggccttc
     2101 gggtcggtgc tcgcccgtaa gagatcgcac gacttggcat aggggtcgtt cgggttgcag
     2161 tcggccacgg ccagcgccgc ttcgagcatg ttcagcgcct tctcgtcctg cccggtgtcg
     2221 cggtaaagca tacccaggtt ttgcagattg aagaagtcgt tgcattgtaa ctgcacggcg
     2281 cgctcgaact gcgtgatcgc gtcggcggtc ttgttcaact tccactcgat atagccgagc
     2341 gcggcacggg cctgcaccgg ctcgtcgggg ttggccgggt tctcctgctt ggccttcacc
     2401 gccgaactga tcacctcctg gtagcccgcg gccaggaccg gcagcgcgtc gtgccggttc
     2461 aggaagtcga tacgtttctc gaaatcggtg tcctgcaact ggccgggcgc cagcttcacc
     2521 gtcgcggcat aatcggcggc ggcggccttc gcctcctggg tggccgaaag ggtgtaagtg
     2581 gccgacatct cctgggtcac gcccggctgc tgggccagcg cttcgtagcg ctcgccgctg
     2641 ctgcgctcga tctcggcggc gatgaacggc gtctgctcat agcggtcgtc catctgctgc
     2701 gagccgcgga tcgccttgag cgcgtcgtcg atgcgcccac ccttggcgta ggccagcgcg
     2761 agttcgttcc agatctcggc gttgccgggg ctgagcgtgt gcgccttctc atactctgcg
     2821 atgccctggg catacttctg cgccgcctgg ccggcttgcg tgctgttctg ggccagcgtg
     2881 tcgccccagt acatgtagag gcggcccagg ttggcgtaat ggtcggtgtt gagcgggttc
     2941 agcgactcgg cggtcagcag agccgactgc gcgcgggtca gcaactgttc gggcggtggg
     3001 ctgccctgga actggtccgt gcgcttcgac cactccatat aggcgcggcc caggaacagg
     3061 taatagaagt cctcgttggg cgcgatctgg atcgccttct cgtaggggcc gatggcgttg
     3121 ggccagcgct gggcgttgtc ataggccaga ccctgcttgt agaacatgtc
//
LOCUS       DASUYC010000633         3202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_791194,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000633 DASUYC010000000
VERSION     DASUYC010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791194"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VF276_10310"
     CDS             <1..322
                     /locus_tag="VF276_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806547.1"
                     /note="catalyzes the formation of D-fructose
                     1,6-bisphosphate from D-fructose 6-phosphate in
                     glycolysis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HEX6607294.1"
                     /translation="YAVGKAAVDYALTGMNAVMPVIVRTSDAPYRWKIAPAPLAKIAN
                     REKKMPKGFISRDGFGITAAARRYLAPLIRGEAPLPYGRDGLPRYVSLKNAAVPRKLA
                     PFQR"
     gene            480..905
                     /locus_tag="VF276_10315"
     CDS             480..905
                     /locus_tag="VF276_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HEX6607295.1"
                     /translation="MKIIARLFVAVALLSAGSFAVAAPGPGDHGRHGHDRGNDRHHDS
                     RRHDERRYDHRRYDSRRDYRRHDDRRYVNRGYVGPRYYGHPHRWERGHRYYGPTYVVR
                     DYGHYHLRHPPRGYHWVRADNDYLLVAIATGVILDLALR"
     gene            1065..3110
                     /locus_tag="VF276_10320"
     CDS             1065..3110
                     /locus_tag="VF276_10320"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011038412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEX6607296.1"
                     /translation="MLQQYGWWIVVACALVAVLYGAFSARWILAQSPGNERMQEIAAA
                     IQEGARAYLNRQYTTIGLVGVVLLLLIGFFLSWPTAIGFLLGALLSGAAGYIGMNVSV
                     RANVRTAEAARGGLSKAMDVAFRGGAITGMLVVGLALLGVAGYWLLLNRMGISGEPAL
                     HALVGFAFGSSLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIADNVG
                     DNVGDCAGMAADLFETYAVTVIATMLLGSLMISEVGANAVLYPLLLGGVSIIASIIGT
                     LFVKVKQGGSIMGALYKGVVVSAVLAAAAFYPITMQLMPDFSHGAMNLWGCTLIGLAL
                     TGGIVWITEYYTGTQYKPVQHIAAASTTGHGTNIIAGLGVSMKSTAWPVLAVCAAIWG
                     AFALGGLYGIAVAATAMLSMAGMIVALDAYGPITDNAGGIAEMADLPPEVRGVTDPLD
                     AVGNTTKAVTKGYAIGSAGLAALVLFADYTHKLAEQGRHITFDLSNPAVIIGLFVGGL
                     IPYLFGAMAMEAVGRCAGSVVEEVRRQFREIKGIMEGTAKPDYSRAVDMLTKGAIREM
                     MIPSLLPVLSPIVVALVVGWLMGRDAGVQALGGMLMGTIVTGLFVAISMCTGGGAWDN
                     AKKYIEDGHHGGKGSDAHKAAVTGDTVGDPYKDTAGPAVNPLIKIINIVALLLVPLL"
BASE COUNT          492 a         1078 c         1076 g          556 t
ORIGIN      
        1 gtacgccgtg ggcaaggccg cggtcgacta cgcgctgacc gggatgaacg cggtgatgcc
       61 ggtgatcgtg cgcacgtccg acgcgccgta ccgctggaag atcgcgcccg cgccgctggc
      121 gaagatcgcc aaccgcgaga agaagatgcc gaagggtttc atcagccgcg acggcttcgg
      181 catcaccgcc gccgcacggc gctatctggc gccgctgatc cggggcgagg cgcccttgcc
      241 ctacggcagg gacggcctgc cgcgttacgt gagcctgaag aacgccgcgg tgccacgcaa
      301 gctggccccg ttccagcgct gagccgggcg gagtctgtga ccttgttcgc gatgctgagc
      361 gccacctgaa accgccctgc cggaatcggc tggcgttcag ccgcagcttc ctacgatcca
      421 tcctgccccg gtcgaacgcc gcattgtcgc ggccattgcc gaggggagga gacccgaaga
      481 tgaaaatcat cgcccgcctg tttgtcgccg tcgccctgct cagcgccggc agtttcgccg
      541 tggccgcgcc cggtcccggt gaccatggcc gtcatggcca cgaccgcggc aatgatcgtc
      601 atcacgacag tcggcgtcat gacgagcggc gctacgacca tcggcgctac gacagccgcc
      661 gcgactaccg tcgccacgat gatcgacgct acgtcaatcg cggctacgtc ggcccgcgct
      721 actacggcca tccgcatcgc tgggagcgcg gccatcgtta ctacggcccg acttacgtcg
      781 tgcgcgacta cggccactac cacctgcgcc acccaccacg tggctaccac tgggtacgcg
      841 cggacaatga ttacctgctg gtcgccatcg ccaccggcgt catcctggac ttggcgctgc
      901 gctaagtccg ccgcctgctg aaccttcagg gccgtgcatt cgtgcatggc ccttttcttt
      961 gccggacagc gctgcgccgc acaacagctc gcgccgcaac gtctgcctat actccccggg
     1021 accgcccgtt ccggcggccg catacaaccc atggggaggc tccgatgctg cagcagtatg
     1081 gctggtggat cgttgttgca tgtgcgttag tggcggtttt gtatggcgcg ttctccgcgc
     1141 gctggatcct ggcgcaatcg ccgggcaacg aacggatgca ggagatcgcc gcagcgatcc
     1201 aggaaggcgc ccgcgcctac ctcaaccgcc agtacaccac catcggcctg gtcggcgtgg
     1261 tgctgctgct gttgatcggt ttctttctga gctggccgac cgcgatcggc ttcctgctgg
     1321 gcgcgctgct gtcgggcgcg gccggctata tcggcatgaa cgtgtcggta cgcgccaacg
     1381 tgcgcaccgc cgaagcagcc cgcggcggtt tgagcaaggc gatggatgtc gccttccggg
     1441 gcggcgcgat caccggcatg ctggtggtcg gcctggccct gctcggcgtg gccggttact
     1501 ggctgctgtt gaaccgcatg ggcatcagcg gtgaacccgc cttgcacgca ctggtcggtt
     1561 tcgccttcgg cagctcgctg atctcgatct tcgcgcgcct cggcggcggc atcttcacca
     1621 agggcgccga tgtaggtgcc gacctggtcg gcaaggtcga ggccggcatt cccgaggatg
     1681 acccgcgcaa cccggcggtg atcgccgaca acgtgggcga caacgtgggc gattgcgccg
     1741 gcatggccgc cgacctgttc gagacctacg cggtgacggt gatcgccacc atgctgctgg
     1801 gcagcctgat gatcagcgag gtgggcgcca acgcggtgct gtatccattg ttgctgggcg
     1861 gcgtctcgat catcgcctcg atcatcggca cgctgttcgt caaggtgaag cagggcggct
     1921 cgatcatggg cgcgctgtac aagggcgtgg tcgtctcggc ggtgctggcc gcggccgcgt
     1981 tctatccgat caccatgcag ctgatgccgg acttcagcca cggcgcgatg aacctgtggg
     2041 gctgcaccct gatcgggctg gccctgaccg gcggcatcgt gtggatcacc gagtactaca
     2101 ccggcacgca gtacaaaccg gtgcagcaca tcgcggcggc ctccaccacc ggtcacggca
     2161 ccaacatcat cgcgggcctg ggcgtgtcga tgaagtccac cgcgtggccg gtgctggctg
     2221 tatgcgccgc gatctggggc gcgttcgccc tgggcggtct gtacggcatc gcggtcgccg
     2281 ccacggcaat gctgtcgatg gccggcatga tcgtggcgct ggacgcctac ggcccgatca
     2341 ccgacaacgc cggcggcatc gccgagatgg ccgacctgcc accggaagtg cgtggcgtca
     2401 ccgatccgct ggacgcggtg ggcaacacca cgaaggcggt gacgaagggc tatgcgatcg
     2461 gttccgcggg cctcgcggcg ctggtgctct tcgccgacta cacgcacaag ctcgcggagc
     2521 agggccgcca catcacgttc gacctctcga acccggcggt catcatcggc ctgttcgtgg
     2581 ggggcctcat cccgtacctc ttcggcgcga tggcgatgga agccgtgggc cgctgcgcgg
     2641 gctcggtggt ggaggaagtg cgccgccagt tccgcgagat caagggaatc atggaaggca
     2701 ccgcgaagcc ggactactcg cgcgcggtcg acatgctcac caagggcgcg atccgggaaa
     2761 tgatgattcc gtcgctcctg ccggtgctct cgcccatcgt ggtggcgctc gtcgtgggct
     2821 ggttgatggg ccgcgatgcc ggcgtgcagg ccctgggcgg catgctcatg ggcacgatcg
     2881 tgacgggcct cttcgtcgcg atctcgatgt gcacgggcgg cggcgcgtgg gacaacgcca
     2941 agaagtacat cgaggacggc caccacggcg gcaagggcag cgatgcccac aaggccgcgg
     3001 tcacgggcga cacggtgggc gatccctaca aggacacggc cggccccgcg gtgaacccgc
     3061 tcatcaagat catcaacatc gtcgcgctgc tgctcgttcc tctgctctaa gagccgcaga
     3121 cgccgttcgc tccacaaagc cggcgagggc tcccccgccg gcttttttat cggtgttatc
     3181 ggtgccggtg tttcggtttc gg
//
LOCUS       DASUYC010000634         5916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_832157,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000634 DASUYC010000000
VERSION     DASUYC010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5916
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832157"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            286..2175
                     /locus_tag="VF276_10325"
     CDS             286..2175
                     /locus_tag="VF276_10325"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX6607297.1"
                     /translation="MRIICPIKQVPVVEHLSFDGTTRTLRRTDVPLEINPFDRRAFTS
                     ALALRAAHGGSVTALTMGPPQARHALGQCLGLGLDAAVHLCGPEFAGSDTCATAVALA
                     AACRRIGFDLIMCGKYTVDAETGQVGPELAELLGIPHVSGVTAVAIDAAGGTLHATRE
                     TDYGHEEVSVPLPALVTAAERLIKPLKPPKDEVAVAALAQAEAEGRVQTWGAADLDLA
                     PEQVGLRGSLTTVTGLREMIIPRTPRLIEAADPEAAAQALLAALRARGLFAPDSAPAQ
                     VQPEASAAPAAKEAPAALAPGADVWVVAETTPGGGLRRVTGELLGEARRLGAVLGGQA
                     VALLLGQDVDDLVPALAAAGAERVLVADNPRLARYTPDAWTNVLAGALAAAPVPPAVV
                     LAPATAMGRDYAPRLAARRTLGLTGDCVGFDVDPEGRLVHLKPAFDGHIVAEILARTT
                     PQVATVRPGLLPPWAGPIVSGIVPEPLTVPTTPPFWTEVGPESREAGTAGIELDDAEI
                     VVCAGTGIGGPEHLGEIGALTAALGAALDLRAAVGVTRKVVDLGWAPRQLQVGLTGRA
                     VAPRLYVAVGLRGNWNHVVGIARSGTIVAINNDPAADIFRAADIGVVGDWQAVSAALI
                     RALTA"
     gene            2227..3675
                     /locus_tag="VF276_10330"
     CDS             2227..3675
                     /locus_tag="VF276_10330"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HEX6607298.1"
                     /translation="MRAEFGRLRVGRGLPRGENRRAAGWTGAAVLALGFAGLILIGTL
                     LLLLPAATVAGTSAPPSVALFTATSAVCVTGLAVVETGSYWSRFGQVVIMLLIELGGV
                     GFGAGATMLFWLIGRNISLNDRRMLREIVPGTNLDQVVRTSVAIVGVSVAIQAVGALI
                     LFAGWLPRYPAGEAAFLALFHAISAFCNAGFDVFGAVGQPGVSMGAERHNPGILMPIT
                     LLVLLGGLGFPVIGELAAWRPRRVRLQHPASGRLPQPRPPLSLNARLVLVAHLGLFVF
                     GIGAFWLLEFSNPRTFGGEPPGGQFLDAVNTSLYPRSVGFGSIPLDSFRSTSLLLLLV
                     LMFVGTASAGTGGGVKVNTVAALFAGAAGTVAGRSRPELFKRTLSQESINKALTVVLI
                     SGIVIVGATFLLALTDPALALSHALFEVVSAFSTVGLTMDTTARLSEAGRVIIELMMF
                     IGRLGPLTLVVLLAARERAPYVTYAEEPVLIG"
     gene            3862..4542
                     /locus_tag="VF276_10335"
     CDS             3862..4542
                     /locus_tag="VF276_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEX6607299.1"
                     /translation="MAGKDFAVIGLGRFGSSLCRTLRALGHDVLGIDQDMAQVQEARP
                     IVTDAVQLDATDEEALRSIGIAHYDAVVVAIGVDVESSILITLLLKEIGVRRVVAKAG
                     SALQQRVLAKVGADVVVFPEADAGYRVAHTLVYPAIADFLDLGEDFVILQVPAQPEWA
                     GRTLAELGMAGESTPPHNGYSGLQVQVLRRGTELICAPRPAERVQPGDVLAVVGRHED
                     LARLVDRR"
     gene            complement(4547..5476)
                     /locus_tag="VF276_10340"
     CDS             complement(4547..5476)
                     /locus_tag="VF276_10340"
                     /inference="COORDINATES: protein motif:HMM:NF024764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6607300.1"
                     /translation="MGDLGRLLKPSALARIVLLYLVVMGLYAWQTRAQDEGINHWLLV
                     TVGLTAMLSLATVAVVFILIVVSRGGLAAWRAGRVQAAGQTGDALERYNRVLARNPNL
                     AEALVARAEILQAAGNPSAALTDLDRAIAVTPHVSNVPDPVLYRAYLERGRLREGRGD
                     PQGALADWGQAGRVQPGAPDPYVLRGRAALETGDWLQGRNELAKGIRLLGHSIDVTQD
                     SAQRAALLNMRGLAFNLLGEHRRALADLEQSLQLQPDAWVTHYNLGATYVNLGHPEQA
                     RAELDQAIALNPGAESVARQSRSYAPLWDSADS"
     gene            complement(5507..>5916)
                     /locus_tag="VF276_10345"
     CDS             complement(5507..>5916)
                     /locus_tag="VF276_10345"
                     /inference="COORDINATES: protein motif:HMM:NF036800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607301.1"
                     /translation="SEGHARTIMMIESREDQLAVLERVINGEYSVRQTEEMVRRVRDG
                     VPLPVEPEPPIEEVAALEAAPDEPARPAPRGPKTRRAPLDAETSVLEDSFRTALKTKV
                     QLSRSREGGRLVIHFYSEEMLENLYRMLVGEPE"
BASE COUNT          780 a         1991 c         2221 g          924 t
ORIGIN      
        1 cacacgctgc aagtgctgcg cgcggcgggc cgcatcaccg acgcccaggt ggccgacagc
       61 cgggcgttta tgcgcgaggc cgcgagcggt gcatgagcgc gcattagcga gagccggcgg
      121 gggcagcggt cgggcggccg ctgcccccgc gctgcgtccg gcaccccgga ctcgggccgg
      181 cgcgctcctg cccgtcgcgc attgacggaa ccgggcgcaa catgctaaag tgcgttgttg
      241 tgaatctgct acgatccctg cttcgtactt gttggagagc tttgcatgcg tatcatttgt
      301 ccgatcaagc aggtgccggt cgtcgagcac ctgagctttg acgggaccac ccgcaccttg
      361 cggcgcaccg atgtgccgct ggagatcaat ccattcgacc ggcgcgcctt cacctccgcg
      421 ctggcgttgc gcgcggcgca cggcggcagc gtgaccgcgc tgaccatggg gccgccgcag
      481 gcccgccacg cgctgggcca gtgcctgggt ttggggctgg acgcggcggt gcacctgtgc
      541 gggccggagt tcgccgggag cgatacctgt gccacggcgg tagccctggc cgcggcctgc
      601 cgcaggatcg gcttcgacct gatcatgtgc ggcaagtaca cggtggacgc cgagaccggc
      661 caggtcgggc cggaactggc cgaactgctg ggcatcccgc acgtcagcgg cgtgacggcg
      721 gtagcgattg atgctgcggg cgggacgttg catgcgaccc gcgagaccga ttacggccac
      781 gaagaggtca gcgtgccgct gcctgcgctg gtcaccgccg ccgaacggct gatcaaaccg
      841 ctgaagccgc cgaaggatga ggtcgccgtg gcggcgctgg cgcaggcgga ggccgagggg
      901 cgcgtgcaga cctggggcgc cgccgatctg gacctggcgc cggagcaggt ggggctgcgc
      961 gggtcgctga ccacggtcac gggtttgcgc gagatgatca tcccgcggac gccccggctg
     1021 atcgaggccg ccgatcccga ggcggcggcc caggcactgc tggcggccct gcgcgcccgt
     1081 ggattgttcg cccccgattc ggcacccgcg caggtgcagc ccgaagcgtc cgcggcgccc
     1141 gccgctaaag aggcaccggc ggcgcttgcg cccggcgccg atgtctgggt cgtggcggag
     1201 acgacacccg gcggcggcct gcgccgggtg acgggtgagt tgctgggcga ggcgcggcgg
     1261 ctgggcgccg tgctgggcgg gcaggcggtg gccctactgc tcggccagga tgtggacgat
     1321 ctcgtgccgg ccctggcggc agcgggcgcc gagcgcgtgc tggtagccga taacccgcgc
     1381 ctggcgcgct acacgcccga tgcctggacc aacgtgctgg ccggggcgct ggcggctgcc
     1441 cccgtgccgc ccgccgtggt gctggccccg gccaccgcca tgggccgcga ttacgccccg
     1501 cgcctggccg cgcgccgcac gctcgggctg accggcgatt gcgtcggctt cgatgtggac
     1561 ccggaggggc gcctggtcca cctcaagccg gcgttcgacg gccatatcgt cgcggagatc
     1621 ctggcgcgga ccacgcccca ggtggcgacg gtgcggcccg gcctgctgcc gccctgggcc
     1681 gggccgattg tgtccggaat cgtgcccgaa ccgctgacgg tgcccaccac cccgccgttc
     1741 tggaccgagg tggggccgga gagccgcgag gcgggcaccg ccgggatcga actcgacgac
     1801 gccgagatcg tggtctgcgc cgggaccggc atcggcgggc cggagcacct gggcgagatc
     1861 ggcgcgctga cggcggcgct gggggccgca ctcgacctgc gcgccgctgt gggcgtcacc
     1921 cgtaaggtgg tcgacctggg ctgggcgccg cgccagttgc aggtagggct gaccgggcgg
     1981 gccgtcgcgc cccggctcta tgtggcggtg ggcctgcggg gcaactggaa ccatgtggta
     2041 ggcatcgccc ggtcgggcac catcgtggcg atcaacaacg acccggccgc cgacatcttc
     2101 cgggccgcgg atatcggcgt ggtgggcgac tggcaggcgg tcagcgccgc gctgatccgc
     2161 gccctcaccg cataacccgc ccggcgggtc ggtatgtcgc gcaaagggga tagttaagga
     2221 gggatggtgc gcgcggagtt tgggagactg cgggtcgggc ggggcctgcc gcgcggcgag
     2281 aaccgccggg ccgccggctg gacgggggcc gccgtgctgg ccctcggttt tgccgggttg
     2341 atcctgatcg ggacgctgtt gctgctgctg cccgccgcga ccgtcgcggg tacgagcgcc
     2401 ccgcccagcg tggccctgtt cacggccacc tcggcggtct gcgtcaccgg cctggcggtg
     2461 gtcgagaccg ggtcgtactg gtcacgcttc ggccaggtgg tcatcatgct gctgatcgaa
     2521 ctgggcgggg tgggcttcgg cgcgggggcc acgatgctgt tctggctgat cggacgcaac
     2581 atcagcctga acgaccggcg gatgctgcgc gagattgtgc cgggcaccaa tctcgaccag
     2641 gtggtgcgca cctcggtggc gattgtgggc gtcagcgtgg cgatccaggc cgtgggggcg
     2701 ctgatcctgt tcgcgggttg gctgccccgc taccccgccg gcgaggcggc cttcctggcg
     2761 ctgttccacg cgatcagcgc gttttgcaac gccgggttcg acgtgttcgg cgcggtgggc
     2821 cagccgggcg tcagcatggg cgccgaacgc cacaaccccg gtatcctcat gccgattacg
     2881 ctgctggtgc tgctgggcgg gctgggcttt ccggtcatcg gcgaactggc cgcctggcgc
     2941 ccgcgccggg tgcgcctcca gcacccggca agcgggcggc tgccgcagcc ccggcctccg
     3001 ctcagcctca acgcccggct ggtgctggtg gcccatttgg ggctgttcgt cttcgggatc
     3061 ggggccttct ggctgctgga gttcagcaac ccgcgcacgt tcggcggcga gccgcccggc
     3121 ggccagttcc tcgacgcggt caacacctcg ctctacccgc gcagcgtcgg cttcggatcg
     3181 atcccgctgg acagctttcg ctcgaccagc cttctgttgc tgctggtgct gatgttcgtc
     3241 ggcaccgcct cggcgggcac gggcggcggg gtcaaggtga ataccgtggc ggcgctgttt
     3301 gccggcgcgg cgggcaccgt cgccgggcga tcccgcccgg agctgttcaa gcgcacgctc
     3361 agccaggagt cgatcaacaa ggcgctgacc gtggtcctga tttcggggat cgtgatcgtc
     3421 ggcgccacgt tcctgctggc gctgaccgac ccggccctgg cgctgagcca tgcgctgttc
     3481 gaggtggtct cggcgttctc caccgtgggc ctgaccatgg acaccactgc gcgcctgtcg
     3541 gaggcggggc gggtgattat cgaactgatg atgttcatcg ggcggctggg gccgctgacg
     3601 ctggtggtgc tgctggccgc ccgcgaacgc gcgccctacg tgacctacgc cgaggagccg
     3661 gtgctgatcg gctaaccggg gatcagcctt gtgccagggc gggtttcaac ccgaaccgcc
     3721 aggggacgcg ctgtaggggc cggtttcaaa ccggcccggc acgggacgcg gtgtaggggc
     3781 cggtttgaaa ccggcccggc aggggacgcg ctgtaggggc cggtttcaaa ccggcccggc
     3841 gcaccgaacg aaaggagaaa tatggcgggg aaggactttg ccgtcatcgg cctggggcgg
     3901 tttggatcca gcctctgccg cacgctgcgc gcgctgggcc atgatgtgct gggcatcgac
     3961 caggatatgg cgcaggtgca ggaggcgcgc cccatcgtga ccgacgccgt gcagcttgac
     4021 gccaccgacg aggaggcgct gcgcagcatc ggcatcgcgc actacgacgc ggtcgtcgtg
     4081 gcgattggcg tggatgtgga gagcagcatc ttgatcacgc tgctgctcaa ggaaatcggc
     4141 gtgcgtcggg tggtcgccaa ggccgggagc gcgctgcaac agcgcgtgct ggcaaaggtc
     4201 ggggccgatg tggtagtttt tccggaggcg gatgccgggt accgggtggc gcataccctg
     4261 gtgtacccgg ccatcgccga tttcctggat ttaggcgagg acttcgtgat cctgcaagtc
     4321 ccggcgcagc ccgagtgggc cgggcgcacc ctggccgaac tgggcatggc cggcgagtcc
     4381 acgccgccgc acaacggcta ttccggcctg caggtgcaag tgctgcggcg gggcacggag
     4441 ctgatctgcg cgccccgccc ggcggaacgg gtgcagcccg gcgacgtgct cgccgtggtc
     4501 ggcaggcacg aagacctggc aaggctggtg gaccggcgct agcggctcag gaatcggcac
     4561 tgtcccacag cggggcgtag gagcggctct ggcgggcgac ggattcggcg ccgggattga
     4621 gggcgatagc ctgatcgagt tcggcgcgcg cctgctcggg gtggcccagg ttgacataag
     4681 tcgcgcccag gttgtagtgc gtgacccagg cgtcgggctg caactgcagc gactgttcga
     4741 ggtcggccag ggcgcggcgg tgttcgccca gcaggttgaa ggccagcccg cgcatgttga
     4801 gtagggcggc gcgctgtgcg ctgtcttgcg tgacatcgat gctatggccc agcaggcgga
     4861 tgcccttcgc cagttcgttg cggccctgca accagtcgcc ggtttcgagc gcggcccgcc
     4921 cgcgcagcac gtaggggtcg ggggcgccgg gctggacgcg gcccgcctgg ccccagtcgg
     4981 ccagggcacc ctgcggatcg ccacggccct cgcgcagccg cccgcgctcc aggtaggcgc
     5041 gatagagcac cggatcgggg acgttgctca catggggcgt cacggcgatg gcccgatcga
     5101 gatctgtcag ggcggcggac gggttgcccg cggcctgcaa gatctcggcg cgcgcgacca
     5161 gcgcctcagc caggttgggg ttgcgcgcca gcacgcggtt gtaccgttcg agcgcgtcgc
     5221 cggtctggcc cgccgcctgc acccgcccgg cccgccaggc ggccaggccg ccccggctca
     5281 ccacgatgag gataaagacc acggccacgg tggccaggga gagcatcgca gtgaggccca
     5341 ccgtgaccag cagccagtgg ttgatgcctt catcctgggc acgcgtctgc caggcgtaga
     5401 ggcccatgac cacaagatag agcagcacga tccgggcaag cgccgacggc ttgagcagac
     5461 ggcccaggtc gcccatagac gatccttttc cggccggcgg ggcgccctac tcgggttcgc
     5521 ccaccagcat gcgatacagg ttctccagca tctcctccga gtagaagtgg atcaccaggc
     5581 gcccgccttc gcggctgcgc gacaattgca ctttcgtctt cagggcggtg cggaagctgt
     5641 cttccaggac gctcgtttcc gcgtcgaggg gcgcgcgccg cgttttcggc cctcgcgggg
     5701 ccgggcgcgc cggctcatcg ggcgcagctt ccagggccgc gacttcctcg atgggcggct
     5761 cgggctccac cggcagcggc accccatcgc gcacccggcg caccatctcc tcggtctggc
     5821 gcacgctgta ttccccgttg atgacccgct ccagcacggc aagctgatcc tcgcgcgatt
     5881 cgatcatcat gatcgtgcgc gcgtggccct cgctga
//
LOCUS       DASUYC010000635         2581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_935139,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000635 DASUYC010000000
VERSION     DASUYC010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935139"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="VF276_10350"
     CDS             <1..802
                     /locus_tag="VF276_10350"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX6607302.1"
                     /translation="PGPQTGQAREQTDYMSTNRPTTKPPTKNGKAGATPPRPAPKPQP
                     AGTRVRPVRPQNAPPPTATPPNRLPAILIGGGAVLVALILLSTMFLNTTPKDTTGSTG
                     STGGAVTTPAAGGSGNTGAVGTGSTAVVETPKGTFKIKLFTDDPAVKGAVNNFRNKVA
                     SGYFDGKIFHRVEDWVIQGGDPLGNGTGGGDMPGEYTSHPFVAGAVGMASTASHAPMV
                     NDSQWFVCKTDCQFLNNNYVIFGQVTEGMDVVNKIAIGDTMTKITMQP"
     gene            806..1312
                     /gene="moaC"
                     /locus_tag="VF276_10355"
     CDS             806..1312
                     /gene="moaC"
                     /locus_tag="VF276_10355"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEX6607303.1"
                     /translation="MDESAPHLTHLDAEGRAQMVDVGAKPPTERVAVARGEVRLLGAT
                     LALIRAGEVAKGDVAAVARIAGIMAAKKTSELIPLCHPLALTSVKVELTLEDPAPDAE
                     RGRVIIEARAKTTGPTGVEMEALTAVSVAALTVYDMIKAVDRGAVIADIRLLEKSGGK
                     SGHWTSGQ"
     gene            complement(1441..>2581)
                     /locus_tag="VF276_10360"
     CDS             complement(1441..>2581)
                     /locus_tag="VF276_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX6607304.1"
                     /translation="LTMAGQTFDPGYIIIDNGKISATGALADLPQPAPDADVVDLTGK
                     WILPGLIDAHTHVGIGEQGFGREGMDTNETTDPVTPQVRAIDATNPDDEAFRDALAAG
                     VTALNVMPGSANVVGGLAFAVKTFGSRIDQMVLRNPTGMKAAMGENPKRTHGDAKRMP
                     STRLGIAALFRDLCVRTQNYMAKQAQAEPGKPFERDLRLEQMALVLRHEIPMRIHAHR
                     ADDILTALRIRDEFGFDMVLDHSTEAFKMPDELLRRNIPAVVGPTLTSRYKVELRERN
                     LRTAGVLVRAGVKVALTTDHWVVPIQHLLLGLMLSVREGLDRDEALKLVTINPAEILG
                     IANRVGSIAPGKDADLAVFSGDPLDIMQRVVRVYLDGEPVYEYTG"
BASE COUNT          444 a          884 c          867 g          386 t
ORIGIN      
        1 gcctggcccg caaaccggcc aggccaggga gcaaacagac tacatgagca ccaatcgtcc
       61 tacgaccaaa cccccgacga agaacggcaa ggccggcgcc accccgcccc gccccgcgcc
      121 caagccgcaa ccggcgggca cgcgcgtgcg cccggtgcgc ccgcagaacg ccccgccccc
      181 caccgcgacc ccgcccaatc gcctgcccgc catcctgatc ggcggcggcg ccgtgctggt
      241 tgccctgatc ctgctgtcca ccatgttcct gaacaccacg cccaaggaca ccaccggcag
      301 caccggcagc actggcggcg ccgtcacgac gcccgccgcg ggcggcagcg ggaacaccgg
      361 cgcggtgggc accggcagca ccgccgtggt cgagacgccc aagggcacct tcaagatcaa
      421 gctgttcacc gacgatcccg cggtgaaggg cgcggtgaac aacttccgca acaaggtcgc
      481 cagcggctat ttcgacggca agatcttcca ccgcgtcgag gactgggtga tccagggcgg
      541 cgacccgctg ggcaacggca ccggcggcgg cgacatgccc ggcgaataca cttcgcaccc
      601 gttcgtggcc ggcgccgtgg gcatggcgtc caccgcctcg catgcgccca tggtcaacga
      661 cagccagtgg ttcgtctgca agacggattg ccagttcctg aataacaact acgtcatctt
      721 cgggcaggtg acggagggca tggacgtggt caataagatc gccatcggcg acaccatgac
      781 caagatcacg atgcagccct agccgatgga cgagtctgct ccgcacctga cccacctgga
      841 tgccgagggc cgcgcgcaaa tggtcgacgt gggcgccaag ccgcccacgg agcgcgtggc
      901 cgtggcgcgc ggcgaggtcc gcctgctcgg cgcgacgctg gcgctgatcc gcgcgggcga
      961 ggtggcgaaa ggcgacgtgg ccgccgtcgc acgcatcgcg ggcatcatgg ccgccaagaa
     1021 gacctccgag ctgatcccgc tgtgccaccc gctggcgctg accagcgtca aggtggaact
     1081 gacgcttgaa gaccccgcgc cggacgccga gcggggccgg gtgatcatcg aggcgcgcgc
     1141 caagaccacc ggcccgacgg gcgtggaaat ggaagcgctc actgcggtca gcgtcgccgc
     1201 cctcaccgtc tacgacatga tcaaggccgt ggaccgtggc gcggtgatcg cggacatccg
     1261 cctgctggaa aaaagcggcg gcaaaagcgg ccattggacg agtggtcagt gatcggtggt
     1321 cagtggtcag taaagcacga ggattagtag caaacagcgg gggcgggtgt gaaacccgcc
     1381 cccgttattt tctacaacgc actaaccgct actgaccact gaccaccgac cactgaccac
     1441 tcacccggtg tactcgtaga ccggctcgcc gtccaggtag acgcggacga cgcgctgcat
     1501 gatgtcgagc gggtcgccgc tgaagacggc caggtcagcg tctttgccgg gggcgatgga
     1561 gcccacccgg ttcgcgatgc ccaggatctc cgccgggttg atcgtgacca gcttgagcgc
     1621 ctcatcacga tccagaccct cacgcaccga gagcatgagg cccagcagca ggtgctggat
     1681 cggcaccacc cagtggtcgg tcgtgagggc tactttcacc cccgcccgca ccagcacgcc
     1741 cgccgtcctg agattgcgct cgcgtagttc caccttgtag cgcgaggtca gcgtggggcc
     1801 gactacggcg gggatgttgc gccgcagcaa ttcgtcgggc atcttgaacg cctcggtcga
     1861 gtggtccagc accatgtcga agccgaactc gtcgcggatg cgcagcgcgg tcaggatgtc
     1921 gtcggcgcgg tgggcgtgga tacgcatggg gatctcgtgg cgcagcacca gggccatctg
     1981 ctccaggcgc aggtcgcgct cgaagggctt gcccggctcg gcttgcgcct gcttcgccat
     2041 gtagttctgc gtccgcacgc agaggtcgcg gaacagggcg gcgataccca ggcgcgtcga
     2101 gggcatacgc ttggcgtcgc cgtgggtgcg cttggggttc tcgcccatgg ccgccttcat
     2161 gccggtggga ttgcgcaaca ccatctggtc gatgcggctg ccgaaggtct tgaccgcgaa
     2221 ggccagcccg ccgaccacgt tggcgctgcc cggcataacg ttgagggcgg tgacgcccgc
     2281 tgctagcgcg tcgcggaaag cctcgtcgtc ggggttggtc gcgtcgatgg cccgcacctg
     2341 cggcgtgacg gggtcggtcg tctcgttggt gtccatgccc tcgcggccaa agccctgttc
     2401 accgatgccg acatgggtgt gcgcgtcgat caggccgggc aggatccatt tgccggtcag
     2461 atccaccaca tcggcgtccg gcgccggttg cggcagatcg gccagcgcgc cggtcgcgct
     2521 gatcttgccg ttgtcgatga tgatgtagcc ggggtcgaag gtctgaccgg ccatggtcag
     2581 c
//
LOCUS       DASUYC010000636         3903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_597080,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000636 DASUYC010000000
VERSION     DASUYC010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597080"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VF276_10365"
     CDS             <1..612
                     /locus_tag="VF276_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607305.1"
                     /translation="WGLYELQPRASVGVPSLPFALDIGGDDYKYLVAGFSGQERTPRS
                     DDPNYWRWTFPDAFLRVPWSDAAKARGGTITLRLSAGPPERAVHAPPPPGTTLRRGEV
                     PDTPLVPAPAQFKLFAGNLRLQPTTPAPGGSDPALVTLQPGGGFQDVTFTVPPTAPTA
                     PASANYLLLHLNSTTWSPADAGVSADDRNLGVQVDSVRVDVAP"
     gene            612..2468
                     /locus_tag="VF276_10370"
     CDS             612..2468
                     /locus_tag="VF276_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607306.1"
                     /translation="MADEIEVPPDATPAEGVPLPVAGTVAGIAMQGSGTDIPFEATPP
                     PPDPPERPKRDGTLVERATLAAFWNAAFLPLKLIVKLLASIVIVRALGLDRFGVVTQL
                     QALLGTLGMISDLGLERALPRFIPEFEMTGGRAGLSRLLRNVSLLKGLTLVPFVLALV
                     LFPDFFITQFNIATERARIVGERGPDYGPLLLGMVALMLVLGAASDVSIQVLYAYFRQ
                     KMTNALDILNALLIPGLQAVLVGLFQLGVFGALVALLIGTAVSVVVSLRLMFRALGDE
                     RLGLRKVTQRAPKAGPAPNGRTPRRPSTRSVWQRFTAYSGMMYVMTLSSALYDQSFVV
                     LALGLLITDNVRLATEVALIGLAFKFVRQLLQALVVPLTGVQTPLFARLYAEDRLDGL
                     RTAYASLTRFLILVLLPAGAGLIIMTRNLLVLLYLQPGKGVVLPPEALPAAVAACAIL
                     TIGLFGESMISIALNVLMVYEDYQAVLIARAFALLSIPLLIVLEPSLGVVGVAAAVSI
                     AALSSRFVALVFGLRRLGLEFPLAFLGRVALATLPILVVIGPLALLLPDVPPKIPSLE
                     WVGLAFANGVLVLAAIFLFWLVFRRLGGLLAEDKQRFAGMRLPGIKLLLRYM"
     gene            2562..3728
                     /locus_tag="VF276_10375"
     CDS             2562..3728
                     /locus_tag="VF276_10375"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX6607307.1"
                     /translation="MTTRTDQLRLNRIILITLAALLILGLAYQARDGLVPFIFALVLG
                     YLLVPLIDLLNRWLPRVAAILLVYVLFFGGLIGLGFLIIPGAAAQVRQLIDSYPAYSE
                     RFMAVTRDVQAWYAALNLPDGVRTQIENGLRNAGGGLLTAVETALFGTLRVVTQAVSF
                     IFGLVVIPFWLFYVMKDKDAAVAKFYNLIPRAVRTDVRRILRIASDVLNDYIRGQLLL
                     GLVVGTATTIGLFIVGAPYWLLLGVLNGFTELIPILGPILGAIPGIIIAALTGDWGLL
                     LKVVVVYVLVQLLENNLLVPKIQGDSVKLHPAIIILALVVGGEIAGLVGVIAAVPVTA
                     IARDVYLYLYKRLGEGYSPRQAESQLPSRQNDEAADEARARQQAKTSADRVSGA"
BASE COUNT          513 a         1324 c         1305 g          761 t
ORIGIN      
        1 tggggcctct acgaactaca gccgcgggcg agtgtgggcg tgccgagcct gcccttcgcg
       61 ctcgacatcg gcggcgacga ttataagtac ctggtggccg ggttctccgg ccaggagcgc
      121 acgccgcgca gcgacgatcc caactactgg cgctggacct tccctgacgc cttcctgcgc
      181 gtgccgtggt cggacgcagc caaggcgcgc ggcggcacca tcaccctgcg cctgagcgcc
      241 gggccgccgg agcgcgccgt ccacgcgccg ccgccgccgg gcaccacctt gcgccggggc
      301 gaggtgcccg atacgccgct ggtgcccgcg cccgcccagt tcaagctctt cgcgggcaac
      361 ctgcgcctcc agcccaccac gccggcgccg ggcggatctg acccggccct ggtgacgctg
      421 caaccgggcg ggggcttcca ggatgtgacc ttcaccgtgc cgccgaccgc gcccaccgcc
      481 ccggccagcg cgaactacct gctgttgcac ctgaattcga ccacctggtc gcccgccgac
      541 gcgggcgtga gcgccgacga ccgcaacctg ggcgtgcagg tggacagcgt gcgcgtggat
      601 gtggcgccct gatggctgac gaaatcgaag tcccgcccga cgccactccc gccgaaggcg
      661 tgcccttgcc cgtcgcgggc acggtggccg gcatcgccat gcaggggtcg ggcaccgata
      721 tcccgttcga ggccacgccg cccccgcccg acccgccgga gcgcccgaag cgcgacggca
      781 cgctggtcga gcgggccacg ctggccgcgt tctggaacgc cgctttcctg cccctgaagc
      841 tgatcgtcaa gctgctggcc tcgattgtga tcgtgcgcgc tctcggtttg gaccgcttcg
      901 gcgtggtgac gcaactccag gctctgctcg gcacgctggg catgatttcc gacctgggcc
      961 tggagcgtgc gctgccgcgc tttatcccag agttcgagat gacggggggc cgggccgggt
     1021 tgagccggct cctgcgtaac gtgagcttgc tcaaagggct gacgctggta ccgtttgtgc
     1081 tcgccctggt gctgttcccg gacttcttca tcacccagtt caatatcgcg acagagcggg
     1141 cgcgcatcgt gggcgaacgc ggcccggact acggccccct cttgctgggc atggtggcgt
     1201 tgatgctggt gctcggcgcg gcctccgatg tctcgatcca ggtgctctac gcctacttcc
     1261 gccagaagat gaccaacgcg ctcgatatcc tcaacgcgct gctcatcccc ggtttgcagg
     1321 cggtactcgt cgggctgttc caactcgggg tcttcggcgc cctggtggcc ttgctgatcg
     1381 ggacggcggt gtcggtggtg gtgtcgctgc gcctcatgtt ccgggcgctg ggcgacgaac
     1441 ggctgggcct gcgcaaggtc acgcagcggg cgccgaaggc cgggccggcg cccaacgggc
     1501 gcacgccgcg ccgcccttcg acccgctcgg tctggcagcg gttcaccgcc tactcgggca
     1561 tgatgtacgt catgacccta tcgtcggcgc tctatgatca gtcgtttgtg gtgctggcgc
     1621 tgggcctgct gatcaccgat aatgtgcgcc tcgccacgga agtggccctg atcgggctgg
     1681 ctttcaaatt cgtgcgccag ttgctgcaag ccctggtcgt gccgctcacc ggcgtgcaga
     1741 cgcctctgtt cgcccggctt tacgccgagg accgcctcga cggcttgcgg acggcttatg
     1801 ccagcctcac ccgcttcttg atcctggtcc tgctacccgc gggggcgggg ttgattatca
     1861 tgacgcgcaa tctgttagtg ctgctttatt tgcagccggg caagggcgtg gtgctgccgc
     1921 cggaagcact gccggcggcg gtggcggcgt gcgcgatcct gaccatcggg ctgttcggcg
     1981 aaagcatgat cagcatcgcg ctgaacgtgc tgatggtcta cgaggattac caggccgtgc
     2041 tgatcgcgcg ggcgtttgcc ctgctcagca tcccgttgct gatcgtgctg gagccctcgc
     2101 tgggggtcgt gggcgtggcc gccgccgtct cgattgcggc gctcagttcc cgcttcgtgg
     2161 ccctcgtctt cggcctgcgg cggttgggtc tggagttccc gctggcgttc ctgggccggg
     2221 tcgcgctggc gacgctgccc attctcgtgg tgatcgggcc gctggccctg ctgttgccgg
     2281 atgtcccgcc caaaatcccc agcctggagt gggtcgggct ggccttcgcc aacggcgtcc
     2341 tggtgctggc ggcgatcttc ctgttctggc tggtcttccg ccgcctgggc gggctgctgg
     2401 ccgaggacaa gcagcgcttc gccgggatgc ggctgccggg catcaagctg ctgttgcgct
     2461 acatgtagcc gggccgtcac gccgcgtcac ggcggcgtcg cgtttcgcgt atcatagtgc
     2521 aaagcattcg cacgcaccgg gcctatgggg gagttgcctt cttgacgaca cgtaccgacc
     2581 agttgcggct gaaccgcatt atcctgatca ccctggccgc cctgctgatc ctggggctgg
     2641 cctaccaggc gcgcgacggg ctggtgcctt tcattttcgc gctggtgctg ggttatttgc
     2701 tggtgccgct catcgacctg ttgaaccgct ggttgccccg cgtggcggcg atcctgctgg
     2761 tctacgtgct cttcttcggg ggcctgatcg gcctgggctt cctgatcatc cccggcgcgg
     2821 cggcccaggt gcgccagctt atcgacagct atcccgcgta cagcgagcgg ttcatggccg
     2881 tcacgcgcga tgtgcaggcg tggtacgcgg cgctcaacct cccggatggt gtgcgcacgc
     2941 agatcgagaa cgggctgcgc aacgcgggcg gcggcctgct caccgccgtt gaaacggccc
     3001 tgttcggcac gctgcgcgtg gtgacgcagg ccgtcagctt tatctttggc ctcgtggtca
     3061 ttcccttctg gctgttctat gtgatgaagg ataaggacgc cgcggtcgcc aagttctata
     3121 acctgatccc ccgcgccgtc cgcacggacg tacgccgcat cttgcgcatc gccagcgatg
     3181 tgctgaacga ctacatccgc ggccagttgc tgctcggact cgtcgtcggc acggccacga
     3241 ccatcggcct gttcatcgtc ggcgcgccct actggctgct gctgggcgtg cttaacggct
     3301 ttaccgagct catcccgatc ctcggcccga tcctgggcgc gatccccggc atcatcatcg
     3361 cggcgctgac cggcgactgg gggctgctgc tcaaagtagt cgtcgtctac gtgctggtgc
     3421 aattgctgga aaacaacctg ctggtgccca agatccaggg cgacagcgtc aagctccacc
     3481 cggcgatcat catcctggcg ctggtggtgg gcggcgagat cgcggggctg gtcggcgtca
     3541 tcgcggcggt gcccgtgacg gccatcgcgc gggatgttta cctctatctc tacaagcgcc
     3601 tgggcgaggg ctacagcccg cggcaagcgg agtcccaatt gccctcgcgc cagaacgacg
     3661 aggccgccga cgaggcccgc gcccgccagc aagccaagac gagtgctgat cgcgtgagcg
     3721 gtgcgtagca cattgctgag tgctgagtac ggacgagtgg tcagtatctg gttgtcatgc
     3781 tgaacgcagt gaagcatctc gtcttcgccc ccacgagccg agactcttcg ctgcgctctt
     3841 ctgaaaatac tatccggccc tggtcaggcg gcttggtcct tgggtttgag cacgacttcg
     3901 ttc
//
LOCUS       DASUYC010000637         2637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1447805, whole genome shotgun sequence.
ACCESSION   DASUYC010000637 DASUYC010000000
VERSION     DASUYC010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447805"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VF276_10380"
     CDS             <1..493
                     /locus_tag="VF276_10380"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX6607308.1"
                     /translation="TAAQVQQAMDALGEDRATYGLIHGDLHQGNYLFAGNQVPALDAV
                     HAIDFELCGWGHYIYDLAVTVSTLLARVDLPALCTGLLHGYRQVRPLPAAHEALIPTF
                     VAARLYGYAHWLAALQDEAVFGDGARVRVTRQIGYLRQFLAGHPLNRWAAAQPGAKGE
                     TEA"
     gene            490..669
                     /locus_tag="VF276_10385"
     CDS             490..669
                     /locus_tag="VF276_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607309.1"
                     /translation="MTGDPKSENQDPQPSPSKAWANLRQPMPLGRKWRLFYRNLWIRV
                     QTRQDCCGHPGQPGC"
     gene            855..1067
                     /locus_tag="VF276_10390"
     CDS             855..1067
                     /locus_tag="VF276_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607310.1"
                     /translation="MFREPALRDEAQLDTFLRRALHAAYDREPPSNAWAALAARLAAP
                     PPPIIPRSTNTARPGLLFIPELLPSG"
     gene            1279..1944
                     /locus_tag="VF276_10395"
     CDS             1279..1944
                     /locus_tag="VF276_10395"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX6607311.1"
                     /translation="MLKQLALGSAGVITGTLVGIYASTIPLLLRRGPGRRKALAGMMT
                     IGGAVEACRATRLTGWDLVAFAQYLTARKFTYSRLNPWDSPAQAFACGQGYCQQQALA
                     LKQIYDRLGIPTRVVFAMRCHFPPGIVDGTWEPGCVSPHSWLRVTLDGEERDVCPGAT
                     SNTPGDYHFEVLSPVRTLQPWMRPFSHLGSVLTNVRRAAVAQRRLHAEQRLFRLEVDS
                     LVL"
     gene            1959..2117
                     /locus_tag="VF276_10400"
     CDS             1959..2117
                     /locus_tag="VF276_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607312.1"
                     /translation="MDDESEGTLGVLAAILLLFTAMLNPVTSVVLAAAGLVGLIVYKM
                     RGRHRPAG"
     gene            complement(2182..2433)
                     /locus_tag="VF276_10405"
     CDS             complement(2182..2433)
                     /locus_tag="VF276_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607313.1"
                     /translation="MIEYITWFLALYPVWMVICGLLLWRSKEYRSNGDALVHGVFTLP
                     FAVGMGILFYIWPMLLFGWGAWPYTAVVLGFTVWLEIRR"
BASE COUNT          451 a          905 c          839 g          442 t
ORIGIN      
        1 gacggcggcg caggttcagc aagccatgga cgcgctgggc gaagatcgcg cgacctatgg
       61 cctgatccac ggcgacctgc accagggcaa ttacctgttc gcgggcaacc aggtgccggc
      121 gctcgatgcg gtgcacgcga ttgatttcga gttgtgcggt tggggccact acatctatga
      181 tctggccgtc accgtctcaa ccttgctggc gcgcgtcgat ctgcccgccc tgtgcacggg
      241 cctgctgcac ggttatcgcc aggttcggcc cttgccggcg gcccacgaag cgctgatccc
      301 caccttcgtg gccgcgcggc tctacggata cgcacattgg ctggccgcgc tgcaagatga
      361 ggcggtcttc ggcgacggcg cccgcgtgcg tgttactcgc cagatcggct atttacggca
      421 gttcctggcc gggcaccccc tcaatcgatg ggcggcagca caaccaggcg cgaaaggaga
      481 aacagaagca tgaccggcga cccgaaatcc gaaaatcaag acccccagcc ttccccatcg
      541 aaagcctggg ccaatctgcg ccagcccatg ccgctggggc ggaagtggcg gctgttctac
      601 cgcaacctct ggatccgcgt ccagacccgg caggactgtt gcggccaccc cggccagccg
      661 ggctgttgaa agtagccccc ggcgcctcgg gtaggcgcct tcgcccggct tccccggaat
      721 acaatatcgc tgttaaggcc atctgattaa accgcgcggg caccgcggtt gtatagggat
      781 ggggcgcacg tcgaagccac cgggacattg acgtacccgg catactggtg cgcctggaaa
      841 gtaggacgat ctccatgttc cgcgaacccg cactgcgcga cgaggcgcag ttggacacct
      901 tcctgcggcg cgcactccac gccgcatacg accgggagcc cccgtccaac gcctgggccg
      961 cgctggccgc ccgcctggcc gcgcccccgc cgccgatcat cccacgctcg acgaacaccg
     1021 cgcgccccgg cctgttgttc atccccgaac tgttgccgtc cggataaacc cgcaccgcaa
     1081 gcaagccgcc gcgccgcagg gcgcggcgac cccttgcccg ccgtgcacca gtccccggtg
     1141 atggaaccgc tactgggcgt ggacttccct tgccggtcct ggttcccggc gtctgcgata
     1201 atgatggcgg gggcgatctg cgactgcgcg ttgctgcccg cgatccgcgc gggtcgcggc
     1261 tggaaagggc gggcaaggat gttgaagcaa ctggcgctgg gaagcgcggg agtgattacc
     1321 ggaaccctgg tgggcatcta tgccagcacg atcccgctgc tgctgcggcg cgggccgggc
     1381 cggcgcaaag cgctcgccgg gatgatgacc atcggcggcg cagtggaagc ctgccgggcc
     1441 acccgtctca ccggctggga tctggtggcc tttgcccagt acctcacggc ccgcaagttt
     1501 acctactccc ggttgaaccc gtgggactcg cccgcgcagg cgttcgcatg cggccagggc
     1561 tattgccagc agcaagccct ggccttgaag cagatctacg accggctggg catcccgacc
     1621 cgtgtggtct tcgccatgcg ctgccacttc ccgccgggga tcgtggacgg tacctgggaa
     1681 ccgggctgcg tctcgcccca cagttggctc cgggtgacgc tggacggcga ggaacgcgat
     1741 gtgtgccccg gcgcaacgag caacacgccg ggcgactatc actttgaagt gctgtccccg
     1801 gtgcgcacgc tccagccctg gatgcgcccg ttcagccacc tgggatcggt gctgaccaac
     1861 gtgcggcggg cggctgtcgc gcaacgccgt ctgcacgcgg agcagaggtt attccgtctg
     1921 gaggtcgatt cgctcgtctt gtgaggaggt gaacactgat ggatgacgaa agtgagggca
     1981 cgctgggcgt gctggcagcg atcctgttac tcttcaccgc catgctgaac ccggttacgt
     2041 cggtagtcct ggcggcggcg ggcctggtgg gcttgatcgt ctacaagatg cggggccggc
     2101 accggccggc ggggtagccg gtccggcacc ggcctcgccg gcgaacagcg gcgccggtct
     2161 ctcgacgctg ccggcaccac gctagcgacg gatctcaagc cacaccgtaa agccgaggac
     2221 gacagcagta taaggccacg cgccccagcc gaacagcagc atcggccaga tatagaacaa
     2281 gatccccatg cctaccgcga aaggcagggt aaagacccca tgcaccaggg catcgccgtt
     2341 tgacctgtac tccttgctgc gccacagcag taacccgcat atcaccatcc acactgggta
     2401 gagcgcaagg aaccacgtga tgtactctat catgcccggc ggccctccca ccgggcgcct
     2461 gtcgcccgca ggcccgcagt acgggcaggg gacatgatcc gggaacaggc tgcgccccgc
     2521 ccgcgccaca cattgttggt actccgatat ttaccaataa cctcgggtct cacggttctt
     2581 ctccctttct aacccatctg ccgggaccct ttcggcagaa atgatctttt tgtgagt
//
LOCUS       DASUYC010000638         3382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1253293, whole genome shotgun sequence.
ACCESSION   DASUYC010000638 DASUYC010000000
VERSION     DASUYC010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253293"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="VF276_10410"
     CDS             complement(<1..448)
                     /locus_tag="VF276_10410"
                     /inference="COORDINATES: protein motif:HMM:NF017633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HEX6607314.1"
                     /translation="MFFDALTIAATRDELLAGLVPGRVQQVRAVGPLAVALEIYGARR
                     RRHLLLSAHAENARVHLLAAPPTRDPTVRSPLLLLLRKYVRGAVLQTIEQPQFERVLD
                     FGFTKVLPVQKHGPPGLDEADQVEEDDEDGVDEPFDDRRTEIPPGFA"
     gene            678..1319
                     /locus_tag="VF276_10415"
     CDS             678..1319
                     /locus_tag="VF276_10415"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX6607315.1"
                     /translation="MDAYVLRPPPPLLVVISGPSGVGKDVTLTRMRELGYKFHYAVTA
                     TTRPRRPTEQDGVDYHFKTVAEFERMRREGELLEWAQVYGHYYGIPKSEVTDYLARGE
                     DVVVKPDVQGAATIRRQAPEAVFIFLAPPSFAEQAERLRLRKTEDPAELALRLRIAGE
                     EMKALPQFDYVVVNHTGKLTETVRQIVCILTAEKCRVHPRQINLQERTATHGL"
     gene            1309..3156
                     /locus_tag="VF276_10420"
     CDS             1309..3156
                     /locus_tag="VF276_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX6607316.1"
                     /translation="MASNPPDVHNLLHQAMDALTRGDKPLARDLLTTVLEMDDRNEQA
                     WLWLSGAVDSPHEQRICLENVLTINPGSTAARQGLAYLEKQEAEHPTPPPAQAAAPQP
                     ATAAPAAQPAAAPQPAMTAPPAAPVVDYPAPQEAAVPAAVAPNYPPAVPPEWTAAPAA
                     PPPGWGNGPAASAPAAPASKEAPASAIPAEWGSAPAAPAASAPGVPQWDAGATGASVP
                     GWDTPAAPAPAWGSPAPPPAWSIPPPSAPAPAPPDADPWAALRADLAAPPTPAPASTP
                     PASNGGMLGAAANSSWMDAFGSGPSAPLASPGPWTGGSSASGTVADNLGVTPFTFDAT
                     GGPPAADNPWSEVEAAVPGLAGFDMGAPSGGEVSLPPDLSGGTSVGGTVLPSDSPWAD
                     TFGGASLGQLPQAGAEEPFLQVRQPDAGHPAYGRENLRGSSPALIARPPIGGRSATPV
                     PTIPCPNCGEMVTDNALSCPRCQYRFYAPCPHCGDYIDTSDPNPRGHDVCPHCQQPVD
                     KKALGQAPARGSIGRPGAPSSTPPTVMVGPTPKNKKKTKSPREDKQAAAVAAAAWTPD
                     PAAAPPRSNAAGRILALIVVLAVLGFVIFVLPHMLNVPTLLTPELTPTP"
BASE COUNT          493 a         1255 c         1169 g          465 t
ORIGIN      
        1 gcgcgaagcc gggcgggatc tcggtccgtc ggtcgtcgaa cggctcgtcg accccgtcct
       61 cgtcgtcctc ctcgacctgg tcggcctcgt ccaggccggg cgggccgtgc ttttgcaccg
      121 gcagcacctt ggtgaagccg aagtcgagca cgcgctcaaa ctgcggctgc tcgatagttt
      181 gcagcacggc gccgcgcaca tatttgcgca gcagcaacag cagcggcgag cgcaccgtgg
      241 ggtcgcgggt gggcggagca gccagcagat ggacgcgggc gttttcggcg tgggccgaga
      301 gcagcaggtg ccggcggcgg cgcgcgccgt aaatctccag ggccacggcc agcgggccga
      361 cggcgcgcac ctgctggacg cgccccggca ccagcccggc gagtagctcg tcgcgggtgg
      421 ccgcaatcgt caaagcatcg aaaaacatag gggcgggcac ctccggtcag ccgtctcttg
      481 cagtatacgc gggccgcgcc tgcttgccaa gcaccggcgt ttccgctata ctgaggaaca
      541 agaacaggct atcgcggcgt gtggcttcgg gtcacgcacc gtgcaggacg ggaaggatgg
      601 gcgagacggc aaccgagatt tcctccgtgc agggtcccga acccatggtt gtgccgcatg
      661 aggcggcggt gcctgagatg gatgcgtatg tcttgcggcc cccgccgccg ttgctggtgg
      721 taatttccgg gccgtcgggt gtcggcaagg atgtcacgct cacccggatg cgcgaactgg
      781 gctacaagtt ccactatgcc gtgaccgcga cgacgcggcc ccgccgcccg accgagcagg
      841 atggggtgga ttatcatttc aagaccgttg ccgagttcga gcggatgcgc cgcgagggtg
      901 agttgttgga atgggcgcag gtctatgggc attactatgg tatccccaaa tcggaagtga
      961 ccgattatct ggcgcgggga gaagatgtgg tggtgaaacc cgacgtgcag ggggcggcga
     1021 cgatccggcg gcaggcgccg gaggcggtgt ttattttcct ggccccgccg tccttcgccg
     1081 agcaggccga gcgcctgcgc ctgcgcaaga cggaagaccc cgccgagttg gccctgcggc
     1141 tgcgcatcgc cggggaagag atgaaggcgt tgccgcagtt tgattatgtg gtggtcaatc
     1201 acaccggcaa gctgaccgaa acggtccgcc agatcgtctg catcttgacc gccgaaaaat
     1261 gccgcgtgca tccccggcag attaacctcc aggagaggac cgcgactcat ggcctctaat
     1321 ccccccgacg tacacaactt gctccaccag gccatggatg cgctcacccg cggcgataag
     1381 ccgctggcgc gcgatctgct gaccaccgtg ctcgaaatgg acgaccgcaa cgagcaggcg
     1441 tggctctggc tgagcggcgc ggtcgattcg ccccacgaac agcgcatctg cctggaaaac
     1501 gtcctgacca ttaatcccgg cagcacggcg gctcgccagg ggctggccta cctggaaaaa
     1561 caagaggcgg aacatcccac gccgccgccc gctcaggccg ctgcgcccca accggcaaca
     1621 gccgcgccgg cagcgcagcc cgctgccgcg ccgcagcccg cgatgaccgc gccgcccgcc
     1681 gccccggtcg tcgactaccc cgcaccgcaa gaagccgcgg tgcccgccgc cgtggcgccc
     1741 aactatcccc cggcggtgcc gccggagtgg accgccgcgc ccgccgcccc gccgcccggc
     1801 tggggcaacg gcccggcagc gtccgcgcct gccgcgcctg cctctaaaga ggcaccggcc
     1861 tccgcgattc cggcggagtg gggcagcgcg cccgccgccc cggcggcctc tgcgcccggc
     1921 gtgccgcagt gggatgccgg cgccacgggg gcatccgtgc cgggctggga tacgcccgcc
     1981 gcgcccgcgc ccgcctgggg cagcccggcc cccccgcctg cctggagcat cccgccgccc
     2041 agcgcccccg cccccgcacc gccggacgcc gacccctggg ccgcgttgcg cgccgatctc
     2101 gcggcgcccc ccacgcccgc ccccgcgtcc acgccgcccg cgagtaacgg cgggatgctg
     2161 ggcgctgccg ccaatagctc ctggatggac gccttcggca gcggccctag cgcgcccctg
     2221 gcgagtcccg gcccctggac cggcggcagt tcggcttcgg gcaccgtggc cgataacctg
     2281 ggcgtcacgc cgtttacgtt cgacgcgaca ggcggcccgc ccgcggcgga taacccctgg
     2341 agcgaggtcg aagccgccgt gccgggcctg gcggggttcg acatgggcgc gccctccggc
     2401 ggcgaggtga gcctgccgcc cgacctcagc ggcggcacgt ccgtgggcgg cacggtgctg
     2461 ccgtcggatt ccccgtgggc cgacacgttc ggcggggcgt cgctggggca gctaccgcag
     2521 gccggcgctg aggaaccctt cttgcaggtg cgccagcccg atgccgggca ccccgcctac
     2581 gggcgcgaga acctgcgcgg gtcgtccccg gcgctgatcg ctcgcccgcc catcgggggc
     2641 cgtagcgcca ctccggtgcc gaccatcccc tgcccgaact gcggcgagat ggtcacggat
     2701 aacgcgctct cctgcccgcg ctgccagtac cgcttctacg cgccctgccc ccactgcggc
     2761 gattacatcg acaccagcga cccgaacccg cgcggccacg atgtgtgccc gcactgccag
     2821 cagccggtcg ataagaaggc gctgggccag gcgcccgcgc gcggctcgat tggccggccc
     2881 ggcgcgccca gcagcacgcc gccgacggtg atggtcggcc ccacgccgaa gaacaagaag
     2941 aagaccaaat cgccgcgcga ggataagcag gccgccgcgg tggccgccgc cgcctggacg
     3001 cctgacccgg ccgccgcgcc cccgcgctcc aatgccgccg ggcgcatcct ggcgctcatc
     3061 gtcgtgctcg ccgtgctcgg cttcgtgatt ttcgtccttc cccatatgct caacgtcccg
     3121 accttgctca cgccggagct gacgccgacg ccatgagccg ccgcggcccg gcgcgggcgg
     3181 gctgaggctc cgtggccgat aaggtgggcg gggtgtccgg gccgccgggc ccgaacgtct
     3241 cgcagaagct gcgcgcccgc cagcgcgttg cgcgcctgct gggcgatctc ctggcgaccg
     3301 acgatcccca ggtgatggac gcctgcgcca ccgggctgct gggcgccggg cacgacttgc
     3361 tggtgatgat cgcccgccgc ct
//
LOCUS       DASUYC010000639         9483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_314604,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000639 DASUYC010000000
VERSION     DASUYC010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 9483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9483
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314604"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>9483
                     /locus_tag="VF276_10425"
     CDS             <1..>9483
                     /locus_tag="VF276_10425"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607317.1"
                     /translation="IDAYRLLGEAYLDLGRPEEAMRFFTHVLNADPQNVLAHIGRAII
                     AEDHHQVDIAIDEYERAFEVDPSITELRGELLRLYAIRYGSAGAAIRTTPSGLAYVHL
                     RAGLRDAAISELGYVAQVRPDRWDVEVALAEALWRNEQLDECAAVAGDILAGHPACVK
                     CKWLLGYLHWSSGRTESGRQYLMDAVALDPAYRIANAIWDATPWPVDRRITRAQTAMV
                     PGWTQEELAGSDLDISVALPPEPPAAPSGSRRVTQKFDTRPPLPGESRRVTQKFDTRP
                     PLPGQTAELTPPAEEAPPVAAAAAEAVEWDSILGSADVPAPFDLDWLDDWAAAAETAP
                     TAPAPTAHATLEFEAITPAATEEGTPDMLGVTPAPEAALSEAVGEAAAPGAVLTEGAA
                     EVAEPEAAAVEAAAESAEPEAALAEATAESAEPEAAPIAVDGIIEQEATEIVVFPVAP
                     AEAAPAGPPVDEAGLPAAEPDGFLAWLSAPATDAPQAEEIAATEPAEDLAVANNAADS
                     PAAQAGMFPAWLDEQPAVAGAAPPADEEDTEAPVAEASSAQPSMFPEWMDTPANAVEE
                     AAPPEGAAGEATANIVEEAAPAETAADATATEPADLAEAGAPAPARHQVVHLFPAWLE
                     EATSDDEDESDLEMLPAWMREAGPLEAGTPAAAAETTADAQVAAPDAAALPAHAEEAA
                     PAAPVPADALSLLTAEQAAPPSIAGEESVASVTEDQEAAPVISLDEPAPFAAAAQGDG
                     ADVTAGTPTETAAAPDAASATIDQSPLIGEPALPVMESVLAEAATDAESETTVAEAGT
                     ASEPAAVAASELDAPILPDDLFPAWLEEIERSEAAGATEAQVGLVSAPEATAEEAAPV
                     IDASPEASTEEAEPVTIAPLETTVEEAAPVMAATVWEAQPAEEPQAAVPEAMSAEDVP
                     ASAAGPEAIPAEDVPANEAAPEAPPTEDIQAGAVAPEDVLAQVALANDVAPEDVPAQV
                     APEAVPTAEAVAMTEPATPPALAAAVSEPDPTAIADADHATVPAAEAPATEDEMFPAW
                     MSETGAASLPVVVAPDVPEAESAESTDREPASAAPVAPVESVAEAEIAAAPADMAQPE
                     IDASAAAETASAQAVAPETPAAEANVLPNWLTEAVNVQAPEATGAPTPLALDEETLFA
                     ADHTFGGQAGEIPMGDDLFPAWLEAGPAGAPDGGELLPSAAAQAAPETPVQEGAEVPL
                     LITAEDAAFPAGQDAAASAPATGFAEASGTPDTMDAVEAEAPGPTAGRAESIPAPPDT
                     GQEADEFPAWMGGSWFEVAVAPAPGETSAEPLVIVPESALESSSAEPVPPVEAGASLP
                     APAGPGAALLDAPAQESPAATPEATQVAAPEAAGDASVAETAAAENELVGAPGFIVAD
                     DAAPVVSYPAEATPEAAEPLHEEILDWPAPPEGSTPAATAEADHPTKPSTAPEETAAL
                     PARTADDEPHDMPTSESALDAAATADTENAEPMASHEAGVEDLALPVLAGPEPGVGAP
                     STLDSAPADAAAPPVDAAAGEAGAETVAQAPDVSAGTAESGPVPTPPPALDHFAKVGY
                     NHGDDSQVPDDTAEPGPSLTADSAAGHSPGPASAPAAVEAGHLPDGVAEHGAPAAGQA
                     AFATSEEDNMAPSYKEDDDQERQFDFDWDRDALPDYLKPFLMNETPGQPGQPPAPAGP
                     AANAAPGAGRPMPPAPQAGGPGNDDFNLDLGNIQPFDFGAASPAPPPGPATPIRPLSA
                     TPPPAAPPPQQAPGGFNESDFGGVQPFNFGAPAGPGGGDEFDFGSDALPSWLGSGGNT
                     GPAFSFDTSAGPQATDQTPGPDLSSPGIPDWLRTPETETPQVARPGPAAPAPPAAPGG
                     DFDFGSEALPSWLSASAAPAPKGAPKGAPQEAPPAANDFGDVQPFDFGAPANQGQPFD
                     FGAMAGGGAPQPFNFSAPATPERGPGPAAGPSQEFDLGGVQPFNFGAPPTPPAAQTTP
                     GGPAPGGDFDFGGIQPFDFGGTTAQAGEASAPQAAGAPGEFDFSMVESFDFRGPGGAP
                     SAGGGNDFDFGGVQPFDFGGTSQAAPAGPAPAGDFDFGGVQPFDFGGAPAGGESAGDV
                     SDVDDFIDFLSVGAGNAAPAVPAPDEFAFDLGGDVQPFSLEGMGLGSTPAAPGPAFGA
                     PPAPGPAFGAPPAPMAGASQGGVRLPAGPGPEEMGSLAGDEFSFEPFMFDAEGGAAPP
                     AAPAPPAFNQAPTMPASPFNPGSAFAGRAPADVPPGLEDLPDLQPFSFEGLDTSSDQG
                     YGFDTGAMGQSFGRRATDRGETSRSGMAFNLEEESSGPAGDVHSFSWQRRSTPAREPA
                     HAPEAPETSGSIFAKARKRKEELEQTLRQEAEARGEVYTPPPVEANLHPEEAAELSYW
                     PNLDEEEEVASAPAQPAVQPFDFNSVAGPTPADVAQDMASLQLAPFEFEEEGPPAAGS
                     PTIQLPEESAQANAQAPAAPEFVADQTIEVPLQDQAGAPAMPEPFAFEAAEIPAEIQP
                     FAFDLGAEAPAAPAEAQAAPPPAEPFAFEAPETGAAEVEPFAFEAPASGPTAVQPFTF
                     EPQEGPPPAVEPFTFEAQESAAATAEPFAFEPEAGEATPVQPFSFEAQETEAAAVQPF
                     TFEAEQGQPTAVEPFTFEAEQGQPTGAEPFTFEAQETEAAPVKPFTFEAPGAGASVEP
                     FIFDTAAPAEPAAQAAPPTFETTEPAAPAVEPFAFDMTEAPEAPAVEPFTFDMAESVA
                     PPSFAPAEPSAVAPPAEAAPQETAAGELLWSIPVAEAGSAPAAAAPPSGGFGEEVAPG
                     EFDFSGLQPFDLGALDLTPEERAYLTGESAPAGVSAQAPAEPAAMAPAPEEPAGIPEL
                     QLQQTEPAGPVEPFSFDMTEFEPPAAEPPAMDLGGVPPAEAPGYEAGAPTATVEPVEF
                     GMSAAGEAAAVEPFQFDTEAAAVEPFQFDTEAPAPAPAEPFQFEPFQFEPEAPPAPEP
                     VQFEAEAAEAEATEPFSFEAPAEPAVTHYETTTRTGTGPLAAETWAEIPAAGAFDFSG
                     VEMDQAPAEPAVAEAPEPVVAQAPAAPAAEAPAEAPAPVPPAPVAEAPAPQEAPVAAQ
                     PAPVPVAPPAPAGGSIDELARHLRENPDDTAGRLALAIGYEQRDDISRAAEQYRLLIK
                     GRNVPENLLDIVTGNLRDLVEAQPDNPSLHRLLGDAYMKQGQY"
BASE COUNT         1541 a         3554 c         3242 g         1146 t
ORIGIN      
        1 atcgacgcct accgcctgct gggcgaagcc tatctggacc tgggccggcc cgaggaagcg
       61 atgcgcttct tcactcacgt gctcaacgcc gacccccaga acgtgctggc ccacatcggc
      121 cgcgccatca tcgccgaaga ccaccaccag gtcgacatcg ccatcgacga gtacgagcgc
      181 gccttcgagg tcgaccccag tatcaccgaa ctgcgcggtg agttgctgcg gctctacgcc
      241 atccgctacg gcagcgcggg cgccgcgatt cgcaccacgc cgagcggcct ggcctatgtc
      301 cacctgcgcg ccgggctgcg cgacgcggcc atcagcgaac tcggctacgt ggcccaggtg
      361 cgccccgacc gctgggatgt cgaggtggcg ctggccgagg ccctctggcg caacgagcag
      421 ttggacgagt gcgccgccgt cgccggcgat attctggccg gccacccggc ctgtgtgaag
      481 tgcaagtggc tgctgggcta tctccattgg agcagcggac gcaccgagag cgggcggcaa
      541 tacctgatgg acgccgtggc gctcgacccg gcctaccgca tcgccaacgc tatctgggac
      601 gccacgccct ggcccgtgga ccggcgcatc acccgcgcgc aaactgccat ggtgcccggc
      661 tggacgcaag aggaactggc cggcagcgat ctggacatca gcgtggcgct gccgccggaa
      721 ccgcccgccg cgcccagcgg ctcgcgccgc gtgacccaga agttcgacac ccgcccgcct
      781 ctgcccggcg agtcgcgccg cgtcacgcag aagttcgaca cccgcccacc cctgcccggc
      841 cagaccgccg aactgactcc cccggcggaa gaagccccac ctgttgccgc ggcagcggcg
      901 gaagcggtgg agtgggacag tatcctgggc agcgcggatg tgcccgcccc cttcgacctt
      961 gattggttgg acgactgggc cgccgccgcc gaaaccgccc ccaccgcgcc cgcgccaacg
     1021 gcccacgcta ccctggagtt cgaggcaatc acgcccgccg ccaccgaaga aggcacgccg
     1081 gatatgctgg gcgtcacgcc cgcgccggaa gccgcgctga gcgaagcggt aggagaagcc
     1141 gcggcgccgg gagccgtcct gaccgaaggg gcagcagaag tagcggagcc ggaagccgcg
     1201 gcggtcgaag cggcagcaga atcagcggag ccggaagccg ctctggccga agcgacagca
     1261 gaatcagcgg agccggaagc cgccccaatc gcagtcgatg gtatcatcga gcaggaagcc
     1321 accgagatcg tggtgttccc ggtggcgccc gccgaggccg cgcctgccgg gccgcccgtg
     1381 gatgaggccg ggttgcccgc cgccgagccc gacggcttcc tggcatggct gagcgcaccc
     1441 gcgacagacg ccccgcaagc ggaggagatc gccgctacgg aaccggcaga ggatctagcg
     1501 gtggccaaca acgccgcgga ttcgcccgcg gcccaggccg gcatgttccc cgcctggctg
     1561 gacgagcagc ccgcggttgc cggcgccgcc ccgcccgcgg atgaggaaga caccgaggcg
     1621 ccggtcgcgg aagcgtcctc cgcccagccc agcatgttcc cggagtggat ggacacgccg
     1681 gccaatgccg tggaagaagc cgccccgcca gagggtgccg ccggcgaggc caccgcgaat
     1741 attgtggaag aagccgcccc ggcagagacc gccgccgatg cgaccgcaac ggagccggcg
     1801 gatctagccg aagccggcgc cccggcgccc gcccgccatc aggtcgtcca cctcttcccg
     1861 gcctggctgg aagaggccac gagcgacgac gaggacgagt cggacttgga gatgctgccc
     1921 gcctggatgc gcgaggccgg cccgctcgaa gccggcaccc cggctgctgc agcggagacg
     1981 acggcagatg cgcaagtcgc cgcgccggac gccgccgccc tgcccgctca cgccgaggag
     2041 gccgcgcccg ccgcacctgt cccggcggac gccctcagct tgctcaccgc cgagcaggca
     2101 gcgccgccca gcatcgccgg cgaggaatcc gtcgccagcg taacggagga tcaggaagcg
     2161 gcgccagtga tctccttgga cgaaccggct ccgttcgcag cggcggcaca aggggacgga
     2221 gcggacgtga cggccggcac gcccaccgaa acggcagccg ccccggatgc ggcatccgct
     2281 accatcgatc agagcccgct gattggcgaa cccgcgctgc cggtcatgga gtcggtcctg
     2341 gccgaagcag ctaccgatgc cgaatccgag accacagtgg ccgaggccgg gaccgcaagc
     2401 gaaccggccg ccgtggccgc gagtgaacta gatgccccta ttctgcccga cgatctgttc
     2461 ccggcctggc tggaggagat cgagcgcagc gaagcggccg gagcaaccga agcgcaagtg
     2521 gggctggtct ccgcgccgga agcgacggcc gaggaagcag cgccggtaat agatgcctcg
     2581 ccggaagcga gtaccgagga agcagagccg gtaacgatcg ctccactgga aaccactgtc
     2641 gaggaagcag cgccggtaat ggccgccacg gtgtgggaag cccagccggc tgaggagccg
     2701 caggccgctg tgccggaagc gatgtctgct gaggacgtgc cggccagcgc tgccgggccg
     2761 gaagcgatac ctgccgagga cgtgccggcg aatgaagccg cgccggaagc gccgccgaca
     2821 gaagatatac aggccggtgc tgtggcgccg gaagacgtgc tggctcaggt cgcgctggcg
     2881 aatgatgtcg cgccggaaga cgtgccggct caggtcgcgc cggaagcggt gcccaccgca
     2941 gaagcggtgg ctatgaccga accggccacc ccgccggccc tggccgctgc tgtgtccgaa
     3001 cccgatccta cagcgatagc ggatgccgac catgcgacgg tgcctgcagc ggaggcgcct
     3061 gccaccgaag acgagatgtt ccccgcctgg atgagcgaga ccggcgcggc cagtttgccg
     3121 gtggtggtcg cgcccgacgt gccggaagcc gaaagcgccg aatccacgga tcgcgaaccg
     3181 gcctcagcgg cgcccgtcgc gcctgtggag tctgtagcgg aagctgaaat cgccgccgcg
     3241 ccggcagaca tggcgcaacc ggagatcgac gcgagtgcag cagcggaaac cgcatcggca
     3301 caagcggtgg cccccgagac gccggcagcg gaagcgaatg tgctgccgaa ttggctcacc
     3361 gaggctgtga atgtgcaggc gccggaggcg accggtgccc ccacgccgct ggcgctggac
     3421 gaagaaacgc tgttcgccgc cgaccacacc ttcggcgggc aggccggcga gatcccaatg
     3481 ggggatgatc tcttccccgc gtggttggag gccggccctg ccggcgcgcc ggacggcggc
     3541 gaactactgc ccagcgcggc agcccaggcc gcgccggaaa cgccggttca ggaaggcgct
     3601 gaggtgccgc tcctgattac ggcggaagat gccgccttcc ccgccgggca agatgctgcg
     3661 gcatccgcac ccgcgaccgg ctttgcagag gcatccggca cgccggacac gatggacgca
     3721 gtggaagcgg aggcgccggg cccgactgcg ggccgcgccg agagcatccc ggccccaccg
     3781 gacaccggcc aagaggcgga cgagttcccg gcgtggatgg gcggatcgtg gttcgaggtc
     3841 gcggttgccc cggcgcccgg cgagaccagt gcggaaccgc tggtgatcgt gcccgagagt
     3901 gcgctggagt cctcctccgc ggaaccggtg ccgccggtcg aggccggagc aagcctcccg
     3961 gcgccggccg ggccgggcgc cgcactgctg gacgcgccgg cccaggaatc gcccgcggca
     4021 acgccggaag ccacccaggt cgccgcgccg gaggcagccg gtgacgcatc ggtcgccgaa
     4081 acggccgcag cggagaatga gctagtcggg gcgccgggct tcatcgtggc cgacgacgcg
     4141 gcgccagtcg tgagctatcc cgccgaggct acgccggaag ctgctgagcc actccacgag
     4201 gagatcctcg attggccggc gccccccgag ggcagcacgc ccgccgccac cgctgaagcc
     4261 gatcatccca ccaaaccgag caccgcgccg gaagagacag ccgcgctccc ggcccgcacc
     4321 gcagacgacg aaccgcacga tatgcccacc agcgagtccg cgctggacgc cgcagcgacg
     4381 gcggacaccg aaaacgccga accgatggcg tcccatgagg ccggagtcga ggacctggcc
     4441 ctgcccgtac tggccggccc ggagccgggc gttggcgccc cctccaccct cgacagtgcc
     4501 cccgcagatg ccgccgcgcc gccggtagat gccgccgccg gggaagccgg cgcggaaacc
     4561 gtcgcgcagg cgccggatgt gtctgccggg acagcggaat ccggcccggt ccccacgccg
     4621 ccgcccgcgt tagatcattt tgccaaagtg ggctataatc atggcgacga ctcacaggtc
     4681 ccagacgaca cggcagaacc cggcccctcg ctcaccgcgg acagcgcggc cggtcactcg
     4741 ccgggtccgg catcggcccc ggccgcagtc gaagccggtc acctgcccga tggagtggcg
     4801 gagcatggcg cgcccgccgc aggccaagca gctttcgcga cctcagagga ggacaacatg
     4861 gctccaagct acaaggaaga cgacgatcaa gagcgccagt tcgacttcga ctgggatcgc
     4921 gatgctctgc ccgattatct caagcccttc ctcatgaatg agactcccgg ccagccgggg
     4981 caaccgccgg cgccggccgg tcccgcggcc aacgccgcgc cgggcgccgg ccggcccatg
     5041 ccgcccgcgc cccaggccgg tggcccaggg aacgacgact tcaacctgga tctgggtaac
     5101 attcagccgt ttgatttcgg cgccgcgtcc ccggccccgc cgcccggccc cgccacgccg
     5161 atccgtccac tgtccgcgac cccgccgccc gcggcccccc cgccgcaaca ggcgccgggt
     5221 ggcttcaacg agagtgattt cggcggggtg cagcccttca acttcggcgc gcccgccggt
     5281 cccggtggtg gcgatgagtt tgacttcggc agcgacgcgc tgccatcctg gttgggatcc
     5341 gggggcaaca ccggccctgc gttcagcttc gacacgtccg ccggcccgca ggccacggat
     5401 cagacgccgg ggcctgattt aagcagtccg gggattcccg attggctgcg caccccggaa
     5461 acggagacgc cgcaggtcgc ccggcccggc cccgccgcgc ccgcgccccc ggccgcgccg
     5521 ggcggcgatt tcgattttgg cagcgaggcg ctgccatcct ggttgagcgc gagcgccgcg
     5581 cctgcaccta aaggggcacc taaaggggca cctcaagagg caccgcccgc cgcgaacgat
     5641 ttcggcgatg tgcagccctt cgacttcggc gcccccgcga accagggcca gcccttcgac
     5701 ttcggcgcca tggccggagg tggcgccccg caaccgttta acttcagcgc gcccgccact
     5761 cccgagcgcg gccccggccc agccgccggg ccgtcgcagg agtttgacct gggcggcgtc
     5821 cagccgttca attttggtgc cccgcctacc ccgcccgccg cgcaaactac acccggtggg
     5881 ccggccccag gcggcgactt tgactttggc ggcatccagc cgtttgattt cggcggcacc
     5941 accgcgcagg caggagaagc ctccgcgccg caagcagccg gcgcgcccgg cgagtttgat
     6001 ttcagcatgg tcgagtcgtt cgacttccgg ggtcccggcg gtgcgccgtc cgcgggcggc
     6061 ggcaacgact tcgatttcgg gggcgtacaa ccgtttgact tcggcggcac cagccaggcg
     6121 gcaccggccg gcccggcccc ggcgggcgac ttcgatttcg gcggcgtcca gccgtttgac
     6181 ttcggcgggg caccggccgg cggcgagagc gccggcgatg tgagcgatgt cgacgacttc
     6241 atcgacttcc tgagcgtggg cgcgggcaac gccgcgcctg ccgtacccgc gcccgacgag
     6301 ttcgccttcg acctgggcgg cgatgtgcaa cccttctcgc tggaaggcat ggggttgggc
     6361 agcacgcccg ccgcccccgg ccccgccttc ggcgccccac cggcgcccgg ccccgccttc
     6421 ggcgccccac ccgcgcccat ggccggcgcg tcgcagggtg gcgtgcgcct gcccgccggg
     6481 cccggccccg aggagatggg cagcctggcc ggcgatgaat tcagctttga gccattcatg
     6541 tttgatgccg agggcggcgc cgcgccgcct gctgcgcccg ccccgccggc cttcaaccag
     6601 gcacccacca tgcccgcgtc gcccttcaat ccggggtcgg cgtttgccgg ccgcgccccg
     6661 gcagatgtgc cgcccggcct ggaggatctg cccgatctcc agcccttctc gtttgagggc
     6721 ctggatacgt ctagcgatca aggttatggg ttcgacaccg gcgccatggg ccaatccttt
     6781 ggccggcgcg cgaccgaccg cggagaaacc agccgcagcg gcatggcctt caacctggaa
     6841 gaagaaagca gcggtcccgc cggggatgtg catagcttct cgtggcagcg ccgcagcacg
     6901 ccggcccgcg aaccggccca tgccccggag gcgcctgaga ccagcggatc catcttcgcc
     6961 aaggcgcgca agcgcaagga ggagctggaa caaaccctgc gccaggaagc ggaagcgcgc
     7021 ggcgaggtct acacgccgcc gccggtggag gcgaacctgc acccggagga agccgcggaa
     7081 ctcagctact ggcccaacct ggacgaagag gaagaagtgg cgagtgcgcc ggcccagccg
     7141 gcggtgcaac ccttcgactt caactcggtg gccggaccca cacccgccga tgtcgcccag
     7201 gatatggcga gcctgcaact ggcgccgttc gagttcgagg aagaaggccc gcccgccgcc
     7261 ggatcgccga ctatccagtt gccggaggaa agcgcgcaag caaacgccca ggcacctgcc
     7321 gcgcccgaat tcgtcgccga ccagaccatc gaggtgccgc tccaggatca ggccggcgcc
     7381 ccggcgatgc ccgaaccgtt cgccttcgag gccgcggaga tccccgccga aatccagccc
     7441 ttcgcgttcg atctgggcgc agaggcgccg gccgcgccgg cggaagcgca agccgccccg
     7501 ccgcccgccg agccctttgc ctttgaggcc ccggagacgg gcgccgccga ggtcgaaccg
     7561 ttcgccttcg aggccccggc cagcggtcct accgccgtcc agcccttcac cttcgagccc
     7621 caggaaggtc cgccgccggc tgtcgaaccg ttcacgtttg aggcccagga aagcgccgcc
     7681 gccaccgccg aaccctttgc ctttgagccg gaagccggcg aggccacgcc tgtccagccg
     7741 ttcagtttcg aggcccagga aaccgaagcg gcggctgttc agcccttcac cttcgaggcg
     7801 gaacaaggtc aacctaccgc cgtcgagccc ttcaccttcg aggcggaaca aggccaacct
     7861 accggcgccg agcccttcac cttcgaggcc caggaaaccg aagcggcgcc tgtcaagccc
     7921 ttcaccttcg aggcacccgg ggccggcgcc tccgtggagc ccttcatctt tgacaccgcc
     7981 gcccccgcgg aaccggctgc gcaagccgcg cctcccactt tcgagacgac cgaacctgcc
     8041 gcgcccgccg tggaaccctt cgccttcgat atgaccgaag cgccggaggc ccccgccgtc
     8101 gagccgttca ccttcgacat ggcggagagc gtcgcgccgc ccagctttgc gccggcggaa
     8161 ccgagtgccg tagccccgcc ggcggaagcg gccccgcagg agacggcggc gggcgaattg
     8221 ctctggagta tcccggttgc ggaagcgggc agcgcgcccg ccgccgcggc ccccccctct
     8281 ggcggcttcg gcgaggaagt ggcacccggc gaattcgact tcagcggcct tcagccattt
     8341 gacctcgggg cgctggatct cacgcccgaa gaacgcgcct acctcaccgg cgagtccgcg
     8401 cccgccgggg tgagcgcgca ggcgcccgcc gaacccgccg ctatggcccc cgccccggag
     8461 gagccagcgg gcattcccga actccagttg cagcagaccg aacccgcggg ccccgtcgag
     8521 ccgttcagct tcgatatgac cgagtttgag ccgcccgccg cggaaccgcc cgcgatggac
     8581 ctgggcgggg tgccgcccgc cgaggcaccc ggctacgagg ccggcgcgcc cacggccacg
     8641 gtcgagccgg tcgagttcgg tatgagcgcg gcgggcgaag ccgccgcggt cgagccgttc
     8701 cagttcgaca ctgaagccgc ggcggtcgag ccgttccagt tcgacaccga agcgcccgcg
     8761 cccgcgcccg ccgagccgtt ccagttcgag ccgttccagt tcgagccgga agcgccgccc
     8821 gcgccggagc cggtccagtt cgaggcggag gctgccgagg ccgaagcgac ggagcccttc
     8881 agcttcgagg cgcccgcgga gccggccgtc acgcactatg aaaccacgac tcgcaccggc
     8941 accgggccgc tggccgccga aacgtgggcg gaaataccgg ccgccggcgc cttcgacttc
     9001 agcggggttg agatggacca ggcgcccgcc gaacctgccg tggccgaggc gcctgaaccc
     9061 gtcgtcgcgc aggcgccggc agcacccgcc gccgaggcgc ccgccgaagc ccccgcgccg
     9121 gtgcccccgg cccccgtcgc cgaggcgcct gccccgcaag aggcaccggt ggccgcccaa
     9181 ccggctccgg tcccggtcgc gccgcccgcc ccggcggggg gctccatcga tgagctggcc
     9241 cgccatctgc gcgagaaccc ggacgacacc gccgggcgcc tggccctggc gatcggctac
     9301 gagcaacgcg acgacatctc gcgcgccgcc gagcaatatc gcctgctgat caagggccgc
     9361 aatgtgccgg agaatctgct cgacatcgtg acgggcaacc tgcgcgatct ggtcgaggcc
     9421 cagcccgata atccgtcctt acatcgcctg ctgggcgacg cctacatgaa acagggccag
     9481 tac
//
LOCUS       DASUYC010000640         4354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1619131, whole genome shotgun sequence.
ACCESSION   DASUYC010000640 DASUYC010000000
VERSION     DASUYC010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1619131"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="VF276_10430"
     CDS             complement(<1..1017)
                     /locus_tag="VF276_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607318.1"
                     /translation="MQPGDYTIDVGLHEATGDLLKRLENVPQGTKVRLNVPRDAITMR
                     TLEDYNMLRDLQKRRQVQLTVASPEPTIIGLARIYGFEVENLSPQKKAPVPAAAVEDE
                     ALPQWNRQGATAPAAAPPDTSWPSYDAASPASNGGSDAETRPQVIRPLAETPAAPPAP
                     APKEAPAPGPTISENDWLFNGVSPTDTQDLPLPAEETAARTSTPPPDVTLPGAVRFVP
                     AGQVPAAPTAPAPAPEADDLGDLDNLNFDSAELDAQSEPYMREIAGRSAAREAAPPVD
                     KDKAKPPAPTRDRGLNGGTAAAAGGAAGGLLAGLGLGRGRKPAANGPTEPSTRTFVDN
                     TEPSH"
     gene            complement(1057..3711)
                     /gene="alaS"
                     /locus_tag="VF276_10435"
     CDS             complement(1057..3711)
                     /gene="alaS"
                     /locus_tag="VF276_10435"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX6607319.1"
                     /translation="MLSSREIRSRFIEFFEQEGHTHVPSSSLVPPGEDTSLLFTNAGM
                     NQFKDVFTGVEQRPYSRAVTVQRCMRAGGKHNDLENVGPSKRHHTFFEMLGNFSFGDY
                     FKRDAISFAWRLFTEVWGLPKDRLWPTVFQDDDDAFNLWQEVAGLPAERITRRGAKDN
                     WWAMGDTGPCGPCSEIFWDFHPELVGEPGHNPEDDEESFIELWNLVFMQFEQVKGGAL
                     VPLPRPSIDTGAGLERVTAILQGTDSNYATDIFAPIMAKIREISGQDEAQMQAQIASY
                     RVIADHGRAVTFLIGDGVQPGPSERGYVLRRVLRRAHRHGRLLGIDHPFLGQIAEVVL
                     ETMGEYAPEILARRDFILRTIRTEEENFQRTLDAGLSRFEALTAGANEGDVVPGGELF
                     RLYDTFGLPRDLIEELVRDRGLQGDWPGYDAAFRQQQEASRAGAAFAQADRADKEIYQ
                     QISPAPTEFLGYDYSALRRPAHVLGIAAKGRRLDSAAAGQEVEVILDRTPFYAEGGGQ
                     VGDTGVLRWPGGRMTVRDTRKPVGGVFVHIGVIDEGELQTGQQVEAIVQEGARWDIMR
                     NHTATHLLHKALRDVLGTHVQQAGSLVAPDRLRFDFSHNAPISREDLARIEHIINEQI
                     IRDYPVDWTVIPYQQALASGAMALFNEKYGNEVRVVHIPGFESRELCGGTHVERTGQI
                     GSCFITSEASIGSGVRRIECVTGRGAVAWVEGRNALLAQVAAAAQAQPERLVPQVVAL
                     QDQLKAAQKRISALERQIASGAAGMRGEQVQEVKGVRVLTARTDAASAESLRDAADHL
                     RDKLQSGVVVLGTVIDGKPSLVAMATADAVGRGANAGQLVKQVAAVVGGGGGGRPELG
                     QAGGRDATKLDDALATVPEFLARQLNGA"
     gene            3900..4238
                     /locus_tag="VF276_10440"
     CDS             3900..4238
                     /locus_tag="VF276_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607320.1"
                     /translation="MDNLAPCPALPTAEGPTLTSDCRVPGSDFGTPADTVRQGAQILQ
                     KIGWEPAQLAQLALYARISPARKIAAMLALRHQAWHVVRDRIACEHPDLAPADLARLV
                     QQQVAAVCDY"
BASE COUNT          634 a         1521 c         1467 g          732 t
ORIGIN      
        1 gtgcgacggc tccgtgttgt cgacaaaggt gcgcgtgctc ggttccgtgg gcccgttggc
       61 cgcgggtttg cgcccgcggc ccagccccag ccccgccagc aggcccccgg cggcgccacc
      121 cgcggcagca gcagtgccgc cgttcagccc gcggtcgcgg gtgggggcgg gtggcttcgc
      181 cttatccttg tcaaccggcg gcgcagcctc gcgggcggca ctccggccgg cgatctcgcg
      241 catgtaaggt tccgattgcg cgtcgagttc ggcggagtcg aaattcaagt tgtcgagatc
      301 gcccaggtcg tcggcttcgg gggcgggcgc gggcgccgtg ggggcggcgg gcacctgccc
      361 cgcgggcacg aaccgcacgg cgccgggcag ggtcacgtcc ggcggcgggg tgctggtgcg
      421 cgcggcggtt tcttcagcgg gcagcggcag gtcctgcgtg tccgtggggc tcacgccgtt
      481 gaacagccag tcgttctcgg agatggtcgg gccgggcgcc ggggcctctt tgggagcagg
      541 cgccgggggc gcggcggggg tctccgccag ggggcgaatg acctggggcc gggtctcggc
      601 gtcgctgccg ccattggagg cgggcgacgc ggcgtcatag ctgggccagc ttgtgtcggg
      661 cggggcggcc gcgggcgcgg tggcgccctg gcggttccac tgcggcagcg cctcgtcttc
      721 caccgcggcg gcgggcacgg gggccttttt ctgcgggctc aggttctcga cttcaaagcc
      781 gtagatccgg gccaggccga tgatcgtcgg ttcgggcgag gccaccgtga gctgcacctg
      841 gcggcgcttt tgcaggtcgc gcagcatgtt gtagtcttcc agcgtgcgca tggtgatcgc
      901 atcgcggggg acattcaggc gcaccttggt gccctgcggc acattttcca gccgcttgag
      961 caggtcgcct gttgcttcat gtaagccaac gtcgatggta tagtcacccg gctgcattgc
     1021 tcgcctcccg gcccctcggc aagagcgtgg tcgcccctac gccccgttca gttgtcgcgc
     1081 caggaattcc ggcacggtcg ccagggcgtc gtccagcttg gtggcgtcac gtccacccgc
     1141 ctgcccgagt tcgggccgcc ccccgcctcc gccgccgact accgcggcca cttgcttcac
     1201 taactgcccc gcgttggcgc cgcgtcccac ggcatcggcg gtcgccatcg ccaccagcga
     1261 gggcttgccg tcgatcacgg tgcccagcac cacgacgccg ctctgcaact tgtcgcgcag
     1321 gtgatcggcg gcatcgcgca ggctctcggc gctcgcggcg tcggtgcgcg cggtcagcac
     1381 gcgcacgccc ttgacttcct gcacctgctc gccgcgcatg cccgccgccc cgctggcgat
     1441 ctggcgctcc agcgcgctga tgcgcttctg cgccgccttc aactggtcct gcaaggcgac
     1501 cacctgcggc acgaggcgct ccggctgggc ctgcgccgcc gccgccacct gggccagcag
     1561 cgcgttgcgg ccttccaccc aggccaccgc cccgcgcccg gtcacgcact cgatgcggcg
     1621 cacgccgcta ccgatggacg cctcgctggt gataaagcac gacccgatct ggcccgtgcg
     1681 ctccacgtgc gtgccgccgc acagttcccg gctctcgaag ccggggatat gcacgacgcg
     1741 cacctcattg ccgtacttct cgttgaacag ggccatggcc ccgctcgcca gcgcctgctg
     1801 gtaggggatc acggtccagt ccaccgggta gtcacggatg atctgctcgt tgatgatgtg
     1861 ctcgatccgt gccaggtctt cccggctgat cggggcgttg tgcgaaaagt cgaaccgcaa
     1921 gcgatccggc gcgaccagcg acccggcctg ctgcacatgg gtacccagca catcgcgcag
     1981 ggccttgtgc aacaggtggg tggcggtgtg gttgcgcatg atgtcccagc gcgcgccctc
     2041 ctgcacaata gcttcgacct gctggccggt ctgcagctcg ccctcgtcaa tcacgccgat
     2101 atggacaaag acgccaccca ccggcttgcg cgtgtcgcgc acggtcatgc gcccgcccgg
     2161 ccaccgcagc accccggtgt cgcccacctg gccgccgccc tcggcgtaga agggcgtgcg
     2221 gtcgagaatg acctcgacct cctgcccggc ggcggcgctg tcgaggcgcc ggcccttggc
     2281 ggcgatgccc agcacgtgcg cgggccggcg cagggcgctg taatcgtagc ccaggaactc
     2341 ggtcggcgcc gggctgatct gctgatagat ttccttgtcg gcgcggtcgg cctgggcaaa
     2401 ggcggcgccc gcccggctgg cttcctgctg ctggcggaac gccgcgtcgt agccgggcca
     2461 gtcgccctgc aacccgcggt cgcgcaccaa ctcctcgatc aggtcacgcg gcaggccgaa
     2521 ggtgtcgtag aggcggaaca gttcgccgcc tggcacaaca tcgccctcgt tggcccccgc
     2581 cgtgagcgcc tcgaaacggc tgagcccggc gtcgagcgtg cgctggaagt tctcctcctc
     2641 ggtgcggatc gtgcgcagga tgaaatcgcg gcgggccagg atctccggcg cgtactcgcc
     2701 catcgtctcc agcaccacct cggcgatctg gcccaggaac gggtgatcga tgcccagcaa
     2761 gcgcccgtgg cggtgcgccc ggcgcagcac gcggcgcagg acatagccgc gctctgacgg
     2821 gccgggctgc acgccgtcgc cgatcaggaa agtcacggcc cggccatggt ctgcgatcac
     2881 ccggtaggag gcgatctgcg cctgcatctg cgcctcgtcc tggccgctga tctcgcggat
     2941 cttggccatg atcggggcga aaatatcggt cgcgtagttg ctgtcggtgc cctgcaggat
     3001 cgcggtcacg cgctccaggc ccgcgccggt gtcgatggag ggccggggca gcggcaccaa
     3061 ggcaccgccc ttcacctgct cgaactgcat gaagacgaga ttccagagct cgataaagct
     3121 ctcctcgtcg tcctcgggat tgtggcctgg ttccccgacc agctccgggt ggaagtccca
     3181 gaagatctcc gagcagggcc cgcaggggcc ggtatcgccc atcgcccacc agttgtcctt
     3241 ggcgccccgg cgcgtgatgc gctcggcggg cagcccggcg acttcctgcc agaggttgaa
     3301 ggcatcgtcg tcgtcctgga acacggtcgg ccagaggcga tccttgggca gcccccagac
     3361 ctcggtgaac aggcgccagg caaagctgat cgcgtcgcgc ttgaaatagt cgccgaagct
     3421 gaagttgccc agcatctcga aaaaggtgtg atggcgcttc gaggggccga cgttctccag
     3481 gtcgttgtgc ttgcccccgg cgcgcatgca gcgctgcacg gtgacggcgc ggctgtacgg
     3541 gcgctgctcc acccccgtga acacgtcctt aaactggttc atgccggcgt ttgtgaacag
     3601 caacgaggta tcctctcccg gcggcaccag ggaggaagac ggcacatgcg tatgcccttc
     3661 ctgctcgaag aattcgataa aacgcgagcg gatctcgcgg ctgcttaaca tggacggacg
     3721 gcctcctgct acgattttat gtcaattaag atgccctgtc aacgggaaca aatgttcgtg
     3781 cccaaaatgg gcataccttg cccagggcac aaatacccac cttgagtata aggatggcaa
     3841 gaggcggtgt caagtttggc gctcttgtgc ccgcccggcg aaaaggttac aatagacgta
     3901 tggacaacct agctccgtgt cccgccctgc caactgcgga aggccctacc cttacttccg
     3961 actgccgtgt tccgggttcc gacttcggta cgcccgccga taccgtgcgc cagggagcac
     4021 agatcctcca aaaaatcggc tgggagccgg cgcaactggc gcaactggcg ctgtatgccc
     4081 gcatctcgcc ggcgcgcaag atcgcggcga tgctggcact gcgccaccag gcgtggcacg
     4141 tcgtccggga tcggatcgcc tgtgaacacc ccgacctcgc ccccgccgac ctcgcgcgcc
     4201 tcgtccagca gcaagtcgcg gcggtctgcg actactaacc ccgaacctaa gtcagaagcc
     4261 ggaacgcgga acgcggcagt tctacctgat ctacttctct ttagcgcgcc cggacaggcg
     4321 ccgggaaagc aacgccgcgc gttaacgggc gcgg
//
LOCUS       DASUYC010000641         3250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1196173, whole genome shotgun sequence.
ACCESSION   DASUYC010000641 DASUYC010000000
VERSION     DASUYC010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196173"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1158)
                     /locus_tag="VF276_10445"
     CDS             complement(106..1158)
                     /locus_tag="VF276_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007127868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX6607321.1"
                     /translation="MSTITARSPARRVSGRPFTIRHFPPVLLAGLLIVAALLVLHISV
                     GTVDVSPGDVVNVLTGQQTAELTHTVVWELRLPRALVAICAGAMLALAGAILQSLTGN
                     PLAEPDLTGASAGGVLFAVLWLSRQLVGWSTAPPSAEVALIALLGAMASGGLVYALSW
                     QRRTSAVRLVLTGVLVATIMRALTSLVLLRNQNAIGGIVLWIIGSLNGRTWTHWDAIW
                     PWALVGLPLGLVCAGAANGLQLGDDIAAGLGMRVERVRALLLFVAVLLTAGAVSVVGA
                     LAFLGLIAPHLARRIAGDDARRVFPLSAVMGAILLLVADIVSRSVAQPNELPVGALMS
                     LLGAPFLMFLLWRGIR"
     gene            complement(1155..2198)
                     /locus_tag="VF276_10450"
     CDS             complement(1155..2198)
                     /locus_tag="VF276_10450"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX6607322.1"
                     /translation="MATLAQISRAVPAARASRVLVLAAVLVVALGGLMMLGLLLGTPQ
                     LAPNDLGTIANGGGDRLARIVVTQLRLPRVLLGAGAGMMLALAGTLLQGTLRNALGSP
                     ELLGVSAGASVVIAAITIFHLELDWPLYPWAALAGGMLAGAVVLLSMRRINDPVRLVL
                     MGVAMNALLYAGIITIITLGQQQDVGLLYLYLLGSLANRTWLQLQVLLPWAAITIPLA
                     LLAARPLNLLQLGDEVAEGLGLRVTRMRMLLLLLSAALVAAIVAVAGPIAFVALMAPH
                     MARRALGTPDARLVLPIAALMGAVLLTLADLVAKNLFDPLELPVGVWTTLIGGPLLLL
                     LLRRELGGRQARA"
     gene            complement(2198..>3250)
                     /locus_tag="VF276_10455"
     CDS             complement(2198..>3250)
                     /locus_tag="VF276_10455"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6607323.1"
                     /translation="AYWESHGGLAQQGYPVSDEFTEVSKLNGKTYTVQYFQRAVLELH
                     PENQAPNDVLLSQLGTFENQARTDLSFTDSTGTKVTLTKRPERIVCLVALCEDILFEL
                     GMEPVGVNDTFGQDPRFWGDKAKSFKSVGSFAAPNLETIASLKPDLVIGFIPHIGLRD
                     ALKPIAPLFVMNPAKYQDSLNFLIKMGLLTGRVYRAQQATQGLLLEMQYFKSKSPNNK
                     VPLLVFGRDQNFSIFTSASLFGSVLADVTNYPWPAPGAGDVSAPDQEPGSIQYSLEKI
                     LQKDPDVLLFETQGAGTKLSQILAANPLWSQLKAVQNKQVYEVNPSMYLFGRGTHSIS
                     IALNDAMHKIYPDVFP"
BASE COUNT          563 a         1152 c         1097 g          438 t
ORIGIN      
        1 actggccggc gcgcaacacc gggtcgccgg ccggcgccgc gatcggggct gcctcgtggg
       61 cgccgttggc ccgcaccagt gtgctctgcg tgccttctcg cttgctcatc ggatccctcg
      121 ccataacaga aacatgagaa acggcgcgcc cagcagcgac atcaacgcgc ccaccggcaa
      181 ctcgttgggt tgcgccacgc tgcgcgacac aatatcggcc accaacagca agatcgcgcc
      241 catcaccgcg ctcagcggga agacccggcg cgcgtcgtcg ccggcgatgc gccgcgccag
      301 gtgcggcgcg atcagcccca ggaaggccag cgcccccacc accgagaccg cgcccgccgt
      361 cagcagcacg gcgacgaaca gcagcagcgc ccgcacacgc tcgacgcgca tgccgagccc
      421 ggcggcgatg tcgtcgccca gttgcaagcc gttggccgcg cccgcgcaga ccagccccag
      481 cggcagcccc accagcgccc acggccagat ggcgtcccag tgggtccagg tgcgcccgtt
      541 gagcgaaccg atgatccaga gcacgatgcc gccgatggca ttctggttgc gcagcagcac
      601 cagcgacgtg agcgcgcgca tgatggtcgc gaccagcacg ccggtgagca ccaggcgcac
      661 cgcgctggtg cgccgctgcc agctcagcgc atagaccagc ccgcccgagg ccatggcgcc
      721 gagcagggcg atcagggcca cctcggcgct gggcggcgcc gtgctccagc ccaccagttg
      781 gcgcgagagc cacagcaccg cgaacagcac gccgcccgcc gaggcgccgg tcaggtcggg
      841 ctcggccagc gggttgccgg tcagcgactg gaggatggcc ccggccagcg ccagcatggc
      901 cccggcgcag atggcgacca gggcgcgcgg caggcgcaac tcccaaacca ccgtgtgcgt
      961 caactcggcg gtctgctgcc ccgtcagcac gttgaccacg tcgcccggcg acacgtccac
     1021 cgtgcccacg ctgatgtgca gcacgagcag ggcggccacg atgagcaacc cggccagcaa
     1081 gaccggcgga aagtgacgga tagtaaacgg tcgtccgcta acccgccggg cgggcgaccg
     1141 ggcggtgatg gtactcatgc gcgcgcctgc ctccccccta gctcgcgccg caacagcaac
     1201 agcagcagcg gcccgccgat cagcgtggtc cagacgccga ccggcaactc cagcggatca
     1261 aacaggtttt tggcgaccag gtcggccagg gtgagcagga cggcgcccat cagcgcggcg
     1321 atgggcaaca ccaggcgcgc gtcgggcgtg cccagggcgc ggcgggccat gtgcggcgcc
     1381 atgagggcca cgaaggcaat cggcccggcc acggcgacga tggccgccac cagcgcggcg
     1441 ctcagcagca gcagcagcat gcgcatgcgg gtcacgcgca gccccagccc ctcggccacc
     1501 tcgtcgccca attgtaacag gttcagcggg cgggcggcca gcagggccag cgggatggtg
     1561 atcgccgccc agggcagcag cacctggagt tgcagccagg tgcggttcgc caggctgccc
     1621 agcaggtaga ggtacagcag gcccacgtcc tgctgctggc ccagcgtgat gatggtgatg
     1681 atccccgcgt agagcagcgc gttcatcgcc acgcccatca gcaccaggcg caccgggtcg
     1741 ttgatgcgcc gcatactcag cagcacgacg gcgcccgcca gcatgccgcc ggccagcgcc
     1801 gcccagggat acaggggcca gtcgagttcg aggtgaaaga tggtgatcgc ggcgatcacc
     1861 accgaggcgc ccgccgagac gcccagcaac tccggcgacc ccagcgcgtt gcgcagcgtg
     1921 ccttgcagca gcgtcccggc cagggccagc atcatgccgg cgccggcgcc cagcagcacg
     1981 cgcggcaggc gcagttgcgt caccacgatg cgcgccagcc tgtcgccgcc gccgttggcg
     2041 atggtgccca ggtcgttcgg cgccaactgg ggcgtgccca gcaggagccc cagcatcatc
     2101 agcccgccca gggccacaac gagaaccgcc gccaggacca gcacgcggct ggccctggcg
     2161 gccgggacgg cgcgagatat ctgcgccagc gtcgccatta cgggaaaacg tccgggtaga
     2221 tcttgtgcat cgcgtcgttc agcgcgatgc tgatcgagtg ggtgccgcgg ccaaacaggt
     2281 acatgctggg gttcacctca tagacctgct tgttctgcac cgccttcaac tggctccaga
     2341 gcggattggc cgccaggatc tgcgacagct tggtgcctgc cccctgggtc tcgaacagca
     2401 gcacgtccgg atccttctgc aggatcttct ccagcgaata ctggatcgag ccgggttcct
     2461 ggtcgggcgc gctcacatcc ccggcgcccg gcgcgggcca cgggtagttg gtcacgtcgg
     2521 ccagcacgct gccgaacaac gacgccgagg tgaagatgct gaagttctgg tcgcggccaa
     2581 ataccaacag cggcaccttg ttgttcggcg acttggactt gaaatactgc atctccagca
     2641 gcagcccctg ggtcgcctgc tgcgcccggt agacgcgccc ggtcaacagg cccatcttga
     2701 tcaggaagtt gagcgagtcc tggtacttgg ccgggttcat gacgaacagc ggggcgatag
     2761 gcttgagcgc gtcgcgcagg ccgatgtgcg ggatgaagcc gatcaccagg tcgggcttga
     2821 gcgaggcgat ggtctccagg ttcggggcgg caaacgagcc gaccgacttg aagctcttgg
     2881 ccttgtcgcc ccagaagcgc gggtcctggc cgaaggtatc gttgacgccc accggctcca
     2941 tgcccaactc aaacagaata tcctcgcaca gtgcgaccag gcagacgatg cgctcggggc
     3001 gcttggtcag cgtgaccttg gtgccggtcg agtcggtgaa cgacaggtcg gtgcgcgcct
     3061 ggttctcgaa ggtgccgagc tggctcagca gcacgtcgtt cggcgcctgg ttctccgggt
     3121 gcaactccag cacggcgcgc tggaaatact gcacggtgta agtcttgccg ttcagcttgc
     3181 tgacctcggt gaactcgtcg gacaccgggt agccctgctg ggccaggccg ccgtggctct
     3241 cccagtaggc
//
LOCUS       DASUYC010000642         3341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1196305, whole genome shotgun sequence.
ACCESSION   DASUYC010000642 DASUYC010000000
VERSION     DASUYC010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196305"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1806
                     /locus_tag="VF276_10460"
     CDS             <1..1806
                     /locus_tag="VF276_10460"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEX6607324.1"
                     /translation="DTTQVETTATTPDYTTTTPGYTTTSPDYTATTTPDYTTPSGYTA
                     TTTQDTPVDTAGEHDAAGDAVGGAGGAVAGGVIGGAVAGPVGAAAGAAIGGAAGAGAA
                     HIAQEGDEGHPGGAETGGAAGALAGGAIGAAVGGPVGAALGAGVGGAVGGGAGGATDD
                     TVDTTRVSSTTTDTGYTAPATADYTAPATTDYTAPAATDYTADTSSYGPSTAYGTEAP
                     SYTTTPDYTTPATETTVDTTTTTGGEAAGDAVGGAGGAVAGGVIGGAMAGPVGAAAGA
                     AIGGAAGAGAAHVAQEGDESHPGGAETGGAAGALAGGAIGAAVGGPVGAAVGAGVGGA
                     VGGGAGGATDDTLTTSQAVSSTTTVPPVAAPAETTCRNCGAPLRPGATFCTRCGTRVV
                     EEAPPRCPNCGEEVTPGDMFCYHCGTRLTGTATSPSVTAPPVAPVAPPPVVTPPAPVP
                     PEPAAPVASAAVAPVAPVEPVAPVAPVTPAPVVPPAPAPSAGTGQPRLELAGSGNVIN
                     LPNKDEILIGREDPLSDPPIFPDVDLTPYGGEEGGVSRRHARILRRDNDYLVEDLQST
                     NYTKLNGQRLAPRQPALLVDGSRVDFGKVSVIFRR"
     gene            1987..>3341
                     /locus_tag="VF276_10465"
     CDS             1987..>3341
                     /locus_tag="VF276_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607325.1"
                     /translation="MAAVRDAIPALRSTTRISIRRLGRTRLLATAAVGLAALALLAWG
                     AWQILAPPLAGESPPLTPLAGVVYEDSARNVNLWVPGEAAPRRLLPPPTGRRELLAVA
                     ADGSALAYLEAVPAATPDSGAAPSDYDLRVWRSGGAPRTVPLPPGATASLDVQFAAGG
                     LLTILQHPTSRRPGPLYLYDPDAGRLRLVAPVLDSFAVSPGLVAYSAPVSDTTQGAAG
                     STRAVSVLAPAGSAPATPVAQFRISAQAPHPVAFYYEPAHNAFVYSAARPTGTQGNNS
                     EIRLGMFLAAAPGKVSDLPLQTNDNSVLELSATGHYLLYGGSLQQVAPGRTARPGSGP
                     ARLAALDWSPSGLAIRSDTAVEPPGFRLTSVTFVRGRDLLAISARRTVTRDLGGLTVP
                     DAGAPVFLLYDPAANRQETVLDNAAATLLEALDSRRLLLAVNAANTGLQNLGAARLVL
                     A"
BASE COUNT          427 a         1332 c         1174 g          408 t
ORIGIN      
        1 gataccactc aggtcgagac cacggccact accccggact acacgacgac cacgcccggc
       61 tacacgacca ccagcccgga ctacacggcg acgaccacgc cggactacac caccccgtcc
      121 ggctacacgg cgacgaccac gcaggacacc cccgtcgata ccgcgggcga gcacgatgcc
      181 gccggcgatg ccgttggggg cgcgggcggg gccgtggccg gtggagtgat tggcggggcc
      241 gtggccggcc cggtcggtgc cgcagcgggt gccgccatcg gcggcgcggc cggcgcgggc
      301 gcggcccata tcgcccagga aggcgacgag ggccatcccg gcggcgcgga aaccggtggg
      361 gcggcaggcg ccctggcggg cggcgcgatc ggcgcagcgg tcggcggccc ggttggggct
      421 gcgctgggcg cgggcgtcgg cggggcggtt ggcggcggcg ccggcggcgc caccgatgat
      481 accgtcgata ccaccagggt gagcagcacg accacggaca ccggctatac cgccccggcg
      541 acggcggact ataccgcccc ggcgacgacg gactataccg ccccggcggc caccgactat
      601 acggcggaca ccagcagcta cgggccgagc acggcctacg gcacggaagc gcccagctat
      661 accaccacgc ccgactacac cactccggcc accgagacca ccgtggacac cacgacgacc
      721 accggcggcg aagcggcggg cgatgccgtg ggcggcgcgg gcggcgcggt ggccggcggc
      781 gtgatcggcg gggcaatggc cgggccggtc ggcgctgctg ccggcgccgc tatcggcggc
      841 gcggccggcg cgggcgccgc gcacgtcgcc caggaaggtg acgaaagcca tcccggcggc
      901 gcggaaaccg gcggcgcggc gggtgccctg gccggcggcg ccatcggcgc ggctgtcggc
      961 ggcccggtcg gcgccgccgt tggtgcgggc gtcggcggcg cggtcggcgg cggcgccggg
     1021 ggcgccacgg acgacacgct cacgacctca caggcggtga gcagcaccac cacggtgccc
     1081 cccgtggccg cgccggcgga aacgacctgc cgcaactgcg gcgcgccgtt gcgccctggt
     1141 gccaccttct gcacccgctg cggcacccgc gtggtcgagg aagccccgcc ccgctgcccc
     1201 aactgcggcg aggaggtcac ccccggcgac atgttctgct atcactgcgg cacccggctg
     1261 acaggcaccg caacctcgcc cagcgtgacg gcaccgcccg tggcgccggt cgccccgccg
     1321 ccggtggtca cgcctccggc gcctgtcccg cccgaaccgg cggcgcccgt ggcgtcggcg
     1381 gcggtcgcgc cggtggcccc ggtggaaccg gtcgcccccg tggcgccggt cacgcccgcg
     1441 cccgtcgtgc cgcccgcgcc ggcgccctcc gccggcaccg ggcagccccg gctcgaactg
     1501 gccggcagcg gcaacgtgat caacctgccg aacaaggacg agatcctgat tggccgcgag
     1561 gatccgctga gcgacccgcc catcttcccc gatgtggacc tcacgcccta cggtggcgag
     1621 gagggcggcg tttcgcgccg gcacgcgcgc atcctgcgcc gcgacaacga ctacctggtc
     1681 gaagacctgc aatcgacgaa ctacaccaag ctcaacggcc agcggctcgc gccgcgccag
     1741 cccgcgctgc tggtggacgg gtcgcgcgtg gacttcggca aagtcagcgt gatcttccgg
     1801 cgttgatcgc cgggcgcttg cgcacgaggg gggcgcgacc tgcgggtcgc gcccctttgc
     1861 ttgcaatccc tgaaccgccg tagcctgcca tctggtataa tccggtgaca ggccgttcgt
     1921 ggcctctgct accggcatac ccttgcttgc cggcgcccgg tgccgtccat attgaggacc
     1981 gatcccttgg ctgctgtgcg cgacgcgatt cccgcgctcc gttccaccac gcgaatatcg
     2041 atccggcgcc tcgggcgcac ccgcctgctg gcaaccgcag ccgtggggct ggcggcgctg
     2101 gccctgctcg cctggggcgc ctggcaaatc ctcgccccgc cgctggcggg ggagtcgccg
     2161 ccgctcaccc cgctggccgg cgtggtctac gaagacagcg cgcgcaacgt caacctgtgg
     2221 gtgcccggcg aagcggcgcc ccgccgcctg ttgccgccgc ccaccgggcg ccgcgaactg
     2281 ctggccgtgg ccgccgacgg cagcgccctg gcctacttgg aagccgtgcc cgccgccacc
     2341 ccggactccg gcgccgcccc ctccgactac gatctgcgcg tgtggcgcag cggcggggcg
     2401 ccgcgcaccg tgcccctgcc gccgggcgcc acggccagcc tcgatgtcca gtttgccgcc
     2461 ggcgggctgc tgaccatcct gcagcacccc acgtcgcgcc gccctggccc gctctatctc
     2521 tacgatcccg acgccgggcg gctgcgcctg gtggcgcccg tcctcgactc gtttgccgtg
     2581 tcgccgggcc tggttgccta ctcggcgccg gtcagcgaca ccacccaggg cgccgccggc
     2641 tccacccgcg ccgtctccgt gctcgcgccc gccggcagcg cccccgccac gccggtcgcg
     2701 cagttccgga tcagtgcgca ggcgccgcac ccggtggcgt tctattacga gccggcccat
     2761 aacgccttcg tctatagcgc cgcccgcccc accggtacgc agggtaacaa cagcgagatc
     2821 cgcctcggca tgttcctggc cgccgcgccc ggcaaggtca gcgacctgcc gctgcagacc
     2881 aacgataact cggtgctcga actgagcgcc accgggcact acctgctcta cggcgggtcg
     2941 ttgcagcagg tggcgccggg acgcaccgcg cggccgggca gcggcccggc ccggctcgcc
     3001 gccctcgatt ggtcgccctc cggcctcgcc atccggtccg atacggcggt ggaaccgccc
     3061 ggcttccggc tcaccagcgt cacgttcgtg cgcgggcgcg acctgctggc gatcagcgcc
     3121 cgccgcaccg tcacccgcga tctcggcggg ctgaccgtgc ccgatgccgg cgcgccggtc
     3181 ttcttgctct acgacccggc cgcaaatcgc caggagaccg tgctcgacaa cgcggcggcc
     3241 accctgctgg aagccctcga ctcgcgccgc ctgttgctcg ccgtgaacgc ggctaatacc
     3301 ggcttgcaga atctcggcgc cgcccgcctg gtgctggccg a
//
LOCUS       DASUYC010000643         4162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1555652, whole genome shotgun sequence.
ACCESSION   DASUYC010000643 DASUYC010000000
VERSION     DASUYC010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1555652"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4132
                     /locus_tag="VF276_10470"
     CDS             <1..4132
                     /locus_tag="VF276_10470"
                     /inference="COORDINATES: protein motif:HMM:NF039478.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6519 domain-containing protein"
                     /protein_id="HEX6607326.1"
                     /translation="ADVIGSCGAPKEGGGFALEVQNGTLAATGGRIYVDGILCENEQA
                     VALTAQPDLPGFTLPADPALYLAFLDVWQRDITALDDPHIREVALGGPDTATRSRVVW
                     QIKLLEVGGENATATCKDDFPAFNDLTGATSGTLTARAQPETGTPNPCVVPPGAGYRR
                     VENQLYRVEIHQGGPVGTATFKWSRENGSIATGWTAQNVNDLTVSTPGRDSVLGFAGG
                     QWIELTDDSHELYDDTQNPTGRPGTLVKLLKVEGQVLTIDPSTAIGSVQFGDFPNNPK
                     IRRWDQPTSGAGALTVEIPATNDGFIALEDGVEIKFETGATYRAGDYWLIPARTITGD
                     IDWPRDSTNAYLPQLPNGIRHHYCRLALARFTAETWQVVEDCRPRFPSLTTICAEDIC
                     FDNTQCQMVDVVTVQDALERLCGESDLRFHNKHLHGWGIVCGLRVTCGPDDEGAPRTH
                     VTVHPGYAIDCEGDDIRLDEDATLDILRMIQILQDRNPNDPILDENGNGEVSMILGLN
                     EARQHLITIEKFRPPGNNFQSLIAGTMLLDVYNDCVKPIQDFLHDQLTPPPDEEKLPA
                     GPGTQRVAALSNLLAQGINPQTGQRLFISRREDAILRDFYTNLRALLQSETFCAMFEN
                     ARPFPDYPAELDQMDTIFAKGEHTRLRLRPGGHEVYSVGAGANPLKPSTKINRFDLDR
                     NVLRSQIDPIAGTETGNQTRDTGTAASGDSGAGAVQDVAFSPNGNRIYMIAPTRNEEN
                     TFFRVGDFDRSGNLTWRPIVTICGVKLITLGTTAADPNNVYAVGVHKVSVTQNGTTRT
                     QIQGLGLYKINPDNVDPNMAPVKAFNSVGHLVITADGRAFATAAPPNTEATSYQAILG
                     IQLPGLTPIFPNRVDTFAVDSGNDDIAVFTRAEGAERETIYVVTGPSQGTKAVQAFDM
                     TGQRIGNPVTVLNTVIRLQTFAPTRMLLMTWEDDYSVRMIDMRSNQIVPNYLLPMQVS
                     PIAITADAARAQRVYVLNYFSNTLTTANGALFRPDFRFPLATLAAYRKGAVEAFADLT
                     AGFLQYLKDCLCDHFLINCPECTGQEKIYLGAVSIRARQVYKICNFTGRKTVGSFPTW
                     GYWLSLVPIVPMIDRMIEQFCCAVLPDIFRYTAPAFDEQQTFNAKSRVQGNTFRSGLG
                     AAQGADLGAWTRNLFNKGQATGNVLNMALRQPQLRAGLAAPTIMTSDIVGQPADEVAN
                     RLKDSGVEVQRAPFDPTSAPALAADLGAFFREPAPGSAVTLHEEDGKVVYYSVAATNP
                     ATQELRARVGTLADSLQAKDAEIQQLRTTLEGQQTTLGEIATLKTSLGDAQTLLEQRD
                     HELEALRGQVADLQRVQTELTTAASPDRVKAIETELEDLRSFRTQVTQFMQQRPPR"
BASE COUNT          781 a         1539 c         1254 g          588 t
ORIGIN      
        1 cgccgacgtt attggctcct gcggcgcgcc gaaggagggc ggcggcttcg cgctggaggt
       61 acagaacggc acgctggccg ccaccggcgg gcggatctac gtggacggca tcctctgtga
      121 aaacgagcag gccgtggcgc tcaccgccca gcccgacctg cccggcttca ccctgcccgc
      181 cgatccggct ctctacctgg cgttcctcga cgtgtggcag cgcgatatca ccgcgctcga
      241 cgacccgcac atccgcgaag tggcgctggg cggccccgac accgccacgc gcagccgcgt
      301 cgtctggcag atcaagctgc tggaggtggg cggcgagaac gccacagcga cctgtaagga
      361 cgactttccc gcctttaacg atctgaccgg cgccacgagc ggcacgctca cggcccgcgc
      421 ccagcccgag acaggcaccc cgaacccctg cgtggtcccg cccggcgccg ggtaccgccg
      481 cgtggagaac cagctctacc gcgtcgagat ccaccagggc gggccggtgg gcaccgccac
      541 tttcaagtgg tcgcgcgaga acggctctat tgccaccggc tggacggccc agaacgtcaa
      601 cgacctgacc gtcagcacgc cgggccgcga cagcgtgctc ggcttcgccg ggggccagtg
      661 gatcgaactg accgacgaca gccacgaact ctacgacgac acccagaacc ccaccgggcg
      721 ccccggcacg ctggtcaagc tgctcaaggt cgagggccag gtactgacca tcgacccgag
      781 caccgccatc ggcagcgtgc agttcggcga cttcccgaat aaccccaaga tccgccgctg
      841 ggaccagccc accagcgggg cgggtgcgct cacggtcgag atcccggcca ccaacgacgg
      901 ctttatcgcg ctggaagacg gcgtcgagat caagttcgag accggcgcca cttaccgcgc
      961 cggcgactac tggctgatcc cggcgcgcac catcaccggc gacatcgact ggccgcgcga
     1021 ctccacgaac gcctacctgc cgcagctgcc gaacggcatc cgccatcact actgccgcct
     1081 ggccctggcc cgcttcacgg ccgagacctg gcaggtggtc gaggactgcc gcccgcgctt
     1141 cccctcgctg accaccatct gcgccgagga catctgcttc gacaacacgc agtgccagat
     1201 ggtcgacgtg gtgacggtgc aggacgcgct ggaacggctg tgcggcgaga gcgatctgcg
     1261 cttccacaac aagcacctgc acggctgggg catcgtctgc gggctgcgtg tcacctgcgg
     1321 ccccgacgac gagggcgcac cgcgcaccca cgtcaccgtc catcccggct acgcgatcga
     1381 ttgcgaaggc gacgacatcc gtctcgacga agacgcgacg ctcgacatct tgcgcatgat
     1441 ccagatcctg caggaccgca accccaacga cccgatcctg gacgaaaacg gcaacggcga
     1501 ggtctcgatg atcctcggcc tcaacgaagc ccggcaacac ctgatcacga tcgagaagtt
     1561 ccgcccgccg ggcaacaact tccagagtct gatcgcgggc accatgctgc tcgatgtgta
     1621 caacgactgc gtcaagccga tccaggattt cctacacgat cagttgaccc cgccgccgga
     1681 cgaggagaag ctgccggcgg gtcccggcac gcagcgcgtg gccgcgctct cgaacctgct
     1741 cgcgcagggc atcaatccgc aaaccggcca gcgcctgttc atctcgcggc gcgaagacgc
     1801 gatcttgcgc gacttctata ccaacctgcg cgcgctgttg cagagcgaga ccttctgcgc
     1861 catgttcgag aacgcccgac ccttccccga ctaccccgcc gaactcgatc agatggacac
     1921 gattttcgcc aagggcgagc acacccggct gcgcctgcga cccggcgggc acgaagtcta
     1981 ctcggtcggc gcgggcgcca atccgctcaa gccgtccacg aagatcaacc gcttcgacct
     2041 ggaccgcaac gtgctgcgca gtcagatcga cccgattgcc ggcaccgaaa ccggcaacca
     2101 gacgcgggat accggcacgg cggcgtcggg cgacagcggc gcgggcgccg tgcaggacgt
     2161 agccttctcg cccaacggca accgcatcta catgatcgcc cccacgcgca acgaggagaa
     2221 caccttcttc cgcgtcggcg acttcgaccg ctcgggcaac ctcacctggc ggcctatcgt
     2281 caccatctgc ggtgtcaagc tgatcaccct gggcaccacc gccgccgacc cgaacaacgt
     2341 ctatgccgtc ggcgtgcaca aagtctcggt gacgcaaaac ggcaccacgc gcacgcagat
     2401 ccagggcctg ggcctgtaca agatcaaccc ggacaacgtc gatcccaaca tggcgccggt
     2461 caaagccttc aacagcgtgg gccacctggt gatcaccgcc gacgggcgcg ccttcgccac
     2521 cgccgcgccg cccaacaccg aggccacatc gtaccaggct attctcggca tccagttgcc
     2581 cggattgacg cccatcttcc ccaaccgcgt ggatacgttc gccgtggaca gcggcaacga
     2641 cgacatcgcc gtgttcacgc gcgccgaggg cgccgagcgc gaaacgatct acgtcgtgac
     2701 cggcccttcg cagggcacca aagcggtcca ggcgttcgac atgaccggcc agcgcatcgg
     2761 caatcccgtc accgtgctga acaccgtgat ccgcctgcaa accttcgcgc ccacgcgcat
     2821 gttgctcatg acctgggagg acgactactc ggtgcgtatg atcgacatgc gcagcaacca
     2881 gatcgtgccg aactatctgc tgcccatgca ggtcagcccc atcgccatca ccgccgatgc
     2941 ggcgcgggcg cagcgcgtct acgtgctcaa ctacttcagc aacaccctga ccacggccaa
     3001 cggcgcgctc ttccgccccg acttccgctt cccgctggcg accctggccg cctaccgcaa
     3061 gggtgcggtc gaggccttcg ccgacctgac cgcgggcttc ctgcaatacc tcaaggactg
     3121 cctgtgcgac cacttcctga tcaattgccc cgaatgcacg ggccaggaaa agatctacct
     3181 gggcgccgtc agcatccgcg cccgccaggt ctacaagatc tgcaacttca ccgggcgcaa
     3241 gacggtcggc agtttcccga cctggggcta ctggctgtcg ctggtgccga tcgtgccgat
     3301 gatcgaccgg atgatcgagc agttctgctg cgcggtgctg cccgacatct tccgctacac
     3361 cgccccggcg ttcgacgagc agcagacctt taacgccaag tcgcgcgtgc agggcaacac
     3421 cttccgttcc ggcctcggcg ccgcgcaggg cgcggacctg ggcgcctgga cgcgcaacct
     3481 gttcaacaag ggccaggcca ccggcaacgt gctgaacatg gcgttgcgcc agccgcaact
     3541 gcgcgccggg ctggccgccc cgacgatcat gacctcggac attgtcggcc agcccgccga
     3601 tgaggtcgcc aaccggctca aggacagcgg agtcgaggtg caacgggccc cgttcgaccc
     3661 gaccagcgcg cccgcgctgg ccgccgacct gggcgccttc ttccgcgagc cggcgcccgg
     3721 cagcgcggtc acgctgcacg aagaggacgg caaagtcgtc tactacagcg tcgccgcgac
     3781 caacccggcc acccaggagt tgcgcgcccg tgtcggcacc ctcgcggact cgctgcaagc
     3841 gaaggacgcc gagatccagc agttgcggac cacgctggaa ggtcagcaga cgacgctagg
     3901 cgagattgcc acgctgaaaa ccagcctggg cgacgcccag acgttgctcg aacagcgcga
     3961 tcacgagctc gaagcactgc gcggccaggt cgcggatctg caacgcgtac agactgagct
     4021 aaccaccgcc gcttcccccg accgggtgaa ggctattgaa acggaactgg aagatttgcg
     4081 gagtttccgc acccaggtga cgcaattcat gcagcagcgc ccgccgcgct aagcgagcgg
     4141 gcgggcaacc gcctatccgg ct
//
LOCUS       DASUYC010000644         2544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_760036,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000644 DASUYC010000000
VERSION     DASUYC010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760036"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..2345)
                     /locus_tag="VF276_10475"
     CDS             complement(138..2345)
                     /locus_tag="VF276_10475"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEX6607327.1"
                     /translation="MRAACALLCVATFLSLVPGGGLPAPPVQAAPVAAPADGFADPAI
                     RAVWARADAAVAAGQANRSWLWGPGPFYTTYEAVNGTPTGVHLVQYFDKGRLEVNDPG
                     ANRNSPWFVTGGLLVREMVQGRISTGAEAGDWEARAPATIPVAGDPGADAAPSYATAR
                     SVVEAPAPGNRTGAPAALVLNAEGTVSSLAKLPAPVTLGAYEPQSRHNVADVFWRYIQ
                     AGGAGPAANWIYTLGLPLTEPFWVRARVGTATRDVLVQLFERRSLTYTPGNAAAWQVE
                     MGNVGRQYYNWRYADLHPDTKTAATRTRYTVQAHVGDDRGLDVVESIHYVNTTAARVP
                     LREVVLRVVYHHWRNAFTVQGAQAAGQTTTTRWRDEVNLAIALPAPLAPGAAVDLELR
                     FGLHPPSFGGRHGYDAANDLLTLGDWLPSVVPYENGGWDQFPYGEHGDQGINEVADYR
                     VTFTSDRAIVVGGTGTTTAHESRRWAFDAPGVRDVAYTISPRLLNPFADGAMTRNAGG
                     VQVLGYFLPGHRAAGGALLDTVASAIRWYSAHIGPYPYRVFTVGEMALPRAAEWDYAQ
                     EYPMVYLIPTQRAGTAPTPGAWTWYTPLHETAHAWFYGAVGNNQLIDPWLDEALANFA
                     SAEYVRSQRPDLYAAAFRAMSGPGGGWPVSASLYSGFPSDDAYFHAVYDQGTQFLAEV
                     RAALGADRFWAAWRDYYAHYTGARARPRDLLTTWQRYSPTDLTPLFNRYLAAW"
     gene            complement(2357..>2544)
                     /locus_tag="VF276_10480"
     CDS             complement(2357..>2544)
                     /locus_tag="VF276_10480"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017687527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX6607328.1"
                     /translation="VTAHVYSFNEPSIRLHERLGFQLEGRLRRMIHTDGQYFDELIFG
                     MTAEEFAARHAGYLAGP"
BASE COUNT          370 a          867 c          952 g          355 t
ORIGIN      
        1 agactcttcg ctccgctcag agtgacaggg aacaggggaa ggcccagcac cagccggcca
       61 cggcagtttc gcgctgtagg ggccggtttc aaaccggccc gccgccccgt tcgcccggcc
      121 cgccgcctac gcggcaacta ccacgccgcg aggtagcggt tgaagagggg agtaagatcg
      181 gtcgggctgt agcgctgcca ggtggtgagc aggtcgcggg ggcgagcgcg ggcgccggtg
      241 taatgcgcgt agtagtcgcg ccaggcggcc cagaagcggt cggcgcccag agccgcgcgc
      301 acctcggcca ggaactgcgt gccctggtca tagacggcgt ggaagtaggc gtcgtcgctg
      361 gggaagccgc tgtagaggct ggcgctcacc ggccagccgc cgccggggcc gctcatcgcc
      421 cggaaggcgg cggcgtagag atcgggccgc tggctgcgca cgtactcggc gctggcgaag
      481 ttggcgaggg cttcgtccag ccaggggtcg atcaactggt tgttgccgac cgcgccgtag
      541 aaccaggcgt gcgccgtctc gtgcagcggc gtgtaccagg tccaggcgcc gggcgtgggc
      601 gcggtgccgg cgcgctgcgt ggggatcagg tagaccatgg ggtattcctg ggcatagtcc
      661 cactcggcgg cgcggggcag ggccatctcc cccaccgtga acacgcggta ggggtacggc
      721 ccgatgtgcg cgctatacca gcggatggcc gaggccacgg tgtcgagcag agcgccgccc
      781 gcggcccggt ggccgggcag gaagtaaccc agcacctgca cgcccccggc gttgcgggtc
      841 atggccccgt cggcgaaggg gttcagcaag cgcggcgaga tcgtgtaggc cacgtcgcgc
      901 acgcccggcg catcgaacgc ccagcggcgg ctctcgtgcg cggtcgtcgt gccggtgccc
      961 ccgacgacga tggcccggtc gctggtgaag gtgacgcggt aatcggccac ctcgttgatg
     1021 ccctggtcgc cgtgctcgcc gtagggaaac tggtcccagc cgccgttctc gtagggcacc
     1081 acgctgggca gccagtcgcc cagcgtcagc aggtcgttgg ccgcgtcata gccgtggcgc
     1141 ccgccaaacg acggcgggtg caggccgaag cgcaactcca gatccacggc ggcgccgggc
     1201 gccagcggcg cgggcagggc aattgccagg ttcacctcgt cgcgccagcg ggtggtcgtc
     1261 gtctgcccgg cggcctgggc accctgcacc gtgaaggcgt tgcgccagtg gtgatagacc
     1321 acccgcagca ccacttcgcg caagggcacg cgcgccgccg tggtgttcac atagtggatg
     1381 ctctcgacca cgtccagccc gcggtcgtcg cccacgtggg cctgcaccgt gtagcgcgtg
     1441 cgggtggcgg cggtcttggt atccggatgc agatcggcgt agcgccagtt gtagtactgg
     1501 cggcccacat tgcccatctc cacctgccag gcggcggcgt tgcccggcgt gtaggtgagg
     1561 ctgcgccgct cgaacaactg gacgagcaca tcgcgcgtgg cggtgcccac gcgggcgcgc
     1621 acccagaacg gctcggtcag cggcaggccc agtgtgtaga tccagttggc ggcgggcccg
     1681 gccccgccgg cctggatgta gcgccagaac acatcggcca cgttgtggcg cgactgcggc
     1741 tcatacgcgc ccagcgtgac gggcgcgggc agcttcgcca gcgacgacac ggtgccctcc
     1801 gcgttcagga ccagggcggc gggcgcgccg gtgcggttgc cgggggccgg cgcctccacc
     1861 acggagcgcg ccgtggcata ggacggcgcg gcatcggcgc cggggtcgcc tgccacgggg
     1921 atcgtcgcgg gggcgcgggc ctcccagtcg ccggcctcgg ctccggtgct gatgcgcccc
     1981 tgcaccatct cgcgcaccag caggccgccg gtgacgaacc agggcgagtt acgattggcg
     2041 ccggggtcgt tgacctccag gcggcccttg tcgaagtact gcaccaggtg gacgccggtg
     2101 ggcgtgccgt tgaccgcttc gtaggtcgtg tagaacgggc cgggccccca gagccagcta
     2161 cgattggcct ggcccgccgc gacggcggca tcggcgcgcg cccagacggc gcggatggcc
     2221 ggatcggcaa agccatcggc gggcgccgcc accggcgccg cctgcaccgg cggcgcgggc
     2281 aaccccccac cgggcaccag actcaggaaa gtcgccacgc agagcagcgc gcaggctgcg
     2341 cgcacggatc gtcgcatcat gggccggcga gatagccggc atggcgcgcg gcgaattctt
     2401 ccgccgtcat cccgaagatc agttcgtcga aatattgccc atcggtgtgg atcatgcgcc
     2461 gcagccgccc ttccagttgg aagcccaggc gctcatggag gcggatggac ggttcgttga
     2521 agctgtaaac atgcgcggtg actt
//
LOCUS       DASUYC010000645         2160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_273159,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000645 DASUYC010000000
VERSION     DASUYC010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273159"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="VF276_10485"
     CDS             complement(<1..1049)
                     /locus_tag="VF276_10485"
                     /inference="COORDINATES: protein motif:HMM:NF020120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase N-terminal
                     domain-containing protein"
                     /protein_id="HEX6607329.1"
                     /translation="MTTDELTPVAQDTAAPPAAPAFSSTAQWIWGGDDPTARNQWRYF
                     RGRFPAPAGVRRATLLLTADSRYECSVNGRYIGRGPARGFPFAYSYDVYDLTPDLRTG
                     ADNVIAVLVNHLADHTMSYIPGAAGLLCEVVLEDAAGNETRYASGTDWRTAVCTTFNN
                     DAPRIATQQEFEEQYDARQEIAGWDQPAFDDSGWAAAYEVAPAGGGPWTGLLPRTIPF
                     LSEDMVWPAEIKAVELARPRPGVFWTLDVRYEAGAERTGQRAAPPGERGLILFTEVFA
                     PQETTVRIHNFPNYEPVGIRVNDQVFDTDDVLGFSDEPIQATLRAGANLVMLKNMLTP
                     SWFFETDVPLSFSADR"
     gene            complement(1071..1943)
                     /locus_tag="VF276_10490"
     CDS             complement(1071..1943)
                     /locus_tag="VF276_10490"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX6607330.1"
                     /translation="MLKSDTRVTSAPLVASQRQERRWRRDWPLNLVLAVLAFLTYVPL
                     VIVLVDSFKDTRQFFTEFWLPALPLHPENYLKALPVISRSLLYTVIYTAPTTFLVLAV
                     SALTGYAFARYRFPGREALFFAVLIIIMLPGILLVIPMFVQIVGWGWQNSVQSIVFPW
                     TAVQIPIGMFLMRTFFETMPKEYFEAARLDGATELQLFYRIALPLALPAFSTLGILTL
                     LFAWNDIIWPLIAIFDATQYPISIGVLAFNSSYSADYGTTFAAYVIASAPLLIFFAFT
                     ARRFMAGLQGGISL"
     gene            complement(1930..>2160)
                     /locus_tag="VF276_10495"
     CDS             complement(1930..>2160)
                     /locus_tag="VF276_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX6607331.1"
                     /translation="SINAGLQEFAPMLIITGGGGPVNATQSPGLYLYQQAFTYNKFGY
                     ATAIGTVLMLITLAFSVFILFARYRRAVDVEV"
BASE COUNT          409 a          687 c          732 g          332 t
ORIGIN      
        1 cggtccgccg agaaactcag cggcacgtcg gtctcgaaga accagctagg cgtcagcata
       61 ttcttcagca tcaccaggtt cgccccggcg cgcaacgtcg cctggatcgg ctcgtcgctg
      121 aaaccgagca cgtcgtcggt atcgaagacc tggtcgttga cacggatgcc caccggctcg
      181 taattgggga aattgtggat gcgcaccgtc gtctcctgcg gggcgaagac ttcggtgaag
      241 aggatcaggc cgcgctcgcc gggcggcgcg gctcgctgcc cggtgcgctc ggccccggcc
      301 tcgtagcgca cgtcgagcgt ccagaagacg ccgggccggg gccgggccag ttcgaccgcc
      361 ttgatctccg ccggccacac catgtcctcg ctcaggaagg ggatggtgcg cggcagcagc
      421 ccggtccagg ggccaccccc cgccggggcc acctcgtatg ccgccgccca gccgctgtcg
      481 tcaaacgccg gctggtccca cccggcgatc tcctgccggg catcgtactg ctcctcaaac
      541 tcctgctgtg tggcgatacg cggcgcgtcg ttgttgaagg tggtgcagac cgccgtccgc
      601 cagtcggtgc cggaagcata gcgggtctcg ttccccgccg cgtcttcaag caccacttcg
      661 cagagcaggc ccgccgcgcc ggggatgtag ctcatcgtgt ggtcggccag gtggttgacc
      721 agcacagcga tcacattgtc cgcgcccgtg cgcaggtcgg gcgtgaggtc atacacgtcg
      781 tagctgtagg cgaaggggaa gccgcgcgcc gggccccggc caatgtagcg gccgttcacg
      841 ctgcactcat agcgcgagtc ggcggtcagc agcagcgtcg cccgccggac ccccgccgga
      901 gcgggaaagc ggccccggaa atagcgccac tggttgcgcg cggtcgggtc gtcgccgccc
      961 cagatccact gggcggtcga agaaaacgcg ggcgcggcgg gcggcgccgc cgtgtcctgc
     1021 gccacgggcg ttaattcgtc tgtcgtcatg gtttgggtcc catcctcagg ttacaggctg
     1081 atgccgccct gcaggccggc cataaagcgg cgcgccgtga aggcgaagaa gatcagcagc
     1141 ggcgccgacg cgatcacgta ggcggcgaac gtagtgccgt agtccgccga atagctcgaa
     1201 ttgaaggcca gcacgccgat cgagatcggg tattgggtag cgtcgaaaat ggcgatcagg
     1261 ggccagataa tgtcgttcca ggcgaacagc agggtcagga tgcccagcgt gctgaacgcg
     1321 ggcagcgcga gcggcagcgc gatgcggtag aacaactgca actcggtcgc gccgtcgagc
     1381 cgcgccgctt caaaatattc cttcggcatg gtctcgaaga aggttcgcat caggaacatg
     1441 ccgatgggga tctgcacggc cgtccagggg aacacgatgg actgaacgct gttctgccaa
     1501 ccccacccga cgatctggac gaacatgggg atcaccagca ggatgcccgg cagcataata
     1561 atgatcagaa cggcgaagaa gagcgcttcg cggcccggaa agcggtagcg cgcgaaagcg
     1621 tagccggtca gcgccgacac cgccagcacc agaaacgtgg tgggcgcggt gtagatcacc
     1681 gtgtagagca gactccgaga aatcaccggc agcgccttga gatagttctc ggggtgcagc
     1741 ggcagcgccg gcagccagaa ctcggtgaag aactggcggg tgtccttgaa cgagtccacc
     1801 agcacgatga ccaggggcac ataggtcaga aacgcgagca cggccagcac caggttcagg
     1861 ggccagtcgc gccgccagcg ccgctcttgc cgctgcgacg ccacgagcgg cgcgctggtc
     1921 acacgggtgt cagacttcaa catcgacggc tctcctgtag cgggcgaaga gaataaatac
     1981 actaaaggcc agcgtaatca gcatcagcac cgtgccgatg gcagtggcgt acccaaactt
     2041 gttgtaggtg aaggcctgct gatacaggta caggcccggc gattgcgtgg cgttgaccgg
     2101 cccgccgccg ccggtgatga tcagcatcgg ggcgaactct tgcaggccgg cgttgatgct
//
LOCUS       DASUYC010000646         5315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_412937,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000646 DASUYC010000000
VERSION     DASUYC010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412937"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..936
                     /locus_tag="VF276_10500"
     CDS             91..936
                     /locus_tag="VF276_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX6607332.1"
                     /translation="MSTPSTSGAPPEPSTSITAPRVIVVMPAYNAARTLEKTYGDMPK
                     EQIHEVILVDDVSSDNTVEVAERLGLKCLVHVQNKGYGGNQKTCYLEALKDGADIVVM
                     LHPDNQYDATRVPAMVAPIAAGQADMVLGSRLMGGASATLRGGMPIWKFISNRFLTTV
                     ENTVFGTRLSEMHTGYRAYSRRMLETIPFLLNSDDFVFDSEVIAQAVAFGFRIVEVPV
                     PTRYFPEASSVNFRRSVTYGLATLNVARRYLFHKLGIRRYKQFSKRLKDVLAKQHQGT
                     IIRQP"
     gene            985..2148
                     /locus_tag="VF276_10505"
     CDS             985..2148
                     /locus_tag="VF276_10505"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX6607333.1"
                     /translation="MLRDKKFWIGVAISIIFLYLTFRGQDFGKIGESLSRVHYWALIP
                     ALACYFGGVWIRALRWGVLLSPVRKGIHPNRLFRFLAIGYMGNDILPARLGDVIRVYI
                     LSRRESVTKSATLATILVERIFDGLTMIGFLAGSALFIQINADLTLALRVSTILFLAG
                     LLVFVFLAAAPQRIVTLIRLILGRSPVGKVIPESLHERALHMTTSFVEGLSVLRNWRG
                     LLSVMGLSVAAWSAEAAMYYIIGAWGFDLQIPVHAYTMTTAAANLGTLVPSSPGYIGV
                     FEGIAIPVLTNVFSLGPHIAASYVVLLHAALYFPVTLLGLFYMVRESVSWSELAALEK
                     KEAAGQPAVEEDDSGDNAGGSGGPGDMLSVSVGGVPAAGVRTGARDAAQSGKR"
     gene            2338..3198
                     /gene="uppP"
                     /locus_tag="VF276_10510"
     CDS             2338..3198
                     /gene="uppP"
                     /locus_tag="VF276_10510"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HEX6607334.1"
                     /translation="MYPLDLIQAIVLGLVQGLAEFIPISSSAHLIIIPWLFGWKDPGL
                     AFDVALHMGTLIAVLVYFWQDWVRLIRGALNSLAQRNLTADPDGKLAWYIVLATIPGA
                     LAGALGESKLDEIFHGDTPAAAANGILIIGVVMIALAALLWIAERIASHRYDMSHLTL
                     QQALTVGVAQAFAIIPGVSRSGSTITAGLFMNLKREAAARFSFLLGTPVIVGAGVKKA
                     YDILKEGGIPSGDLPAFIVGFLVAAISGYAAIFLLLRFLQRNTTLPFIVYRVVVGALL
                     IVFVLAGFRR"
     gene            3238..>5315
                     /locus_tag="VF276_10515"
     CDS             3238..>5315
                     /locus_tag="VF276_10515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX6607335.1"
                     /translation="MVEATPAARAGIGGAALALALLGQALLDRRATFGETTGWLVLAG
                     AASLAVWALGPRALPAPPAQAASATERAGRLRLLILAGAVICGLAALPGFIQLAQTGG
                     QAAPRAGLPWLAWLVSIGLFIVAALVVPHPNSAGRQAGLKPAPTPQNQNGELLIARPA
                     QPPPDSATPAPAPPLSARLRAALPWIAIIGAAAFLRLWQLDSVPRGLWADEAIAGLQA
                     EDILYHGWRPLFIGGANGEPALYLYGIAVLIKFLGPTVVAVRLPMALAGTLAVAALGW
                     LAAPLYGRRVALVAAALLAAMTWQINFSRIGFNAAWSLPVDLLAAGCLLRALATGRLR
                     WYAGAGLFFGLGFQLYYISRAWLLLLAGLLVYRLLTERGLWRGARAGLAWTGLGLLLA
                     AAPVLVYAVIQPADYAARAASVSIADAIQGGGSLAPLGETLLKHLRMWNVMGDFNGRH
                     NLPDARLLDGVTAALLPVGLALAVAATWARRRGWRIDWRFLFPVAGLVVLLAGGVLSN
                     PGDAPQALRTLGDTSMVALLAALPLAAWWATLDGITWRPARRVAVGALAVLGVLLVVF
                     VLNYRRYFFVQEPNPAVYKEFSTPATLVYEMVAAQGPDTDYYLSRGLYEQATIEYLAG
                     GRIGTLFSGLHETPLPPGAPGRPAMLFLNNRDSGALDWLRTLYPNAKIDSVTGPQGGN
                     ALFFAVTVPPA"
BASE COUNT          770 a         1821 c         1738 g          986 t
ORIGIN      
        1 tgggtgtttg ccggggttcc ggttttacgc tgtataccag gggataactg agttagggtc
       61 gtattaacta ttttggggtt ggaggttcgc atgtccacgc cctctacatc cggggcaccg
      121 ccagagccga gtacctcgat caccgctccg cgagtgatcg tcgtcatgcc ggcctataat
      181 gcagcgcgca cgctggaaaa aacctacggc gatatgccga aagagcagat ccacgaggtc
      241 atcctggtcg acgatgtatc gtccgacaac accgtcgaag tcgccgagcg gctgggcctg
      301 aaatgcctgg tccatgtgca gaacaaaggc tacggcggca accagaagac gtgctacctg
      361 gaagcgctga aggacggcgc cgatatcgtg gtcatgctgc atcctgataa ccagtacgac
      421 gccacgcgcg tgccggccat ggtcgcgccg attgccgcgg ggcaggccga catggtgctg
      481 ggctcgcggc tgatgggcgg cgcctcggcc acgctgcggg gcggcatgcc gatctggaag
      541 tttatcagca accgcttcct caccacggtc gaaaataccg tcttcggcac gcgcctctcg
      601 gagatgcata ccggctaccg cgcctacagc cggcgcatgc tggagaccat ccccttcctg
      661 ctcaattccg acgatttcgt attcgacagc gaggtgatcg cccaggcggt cgctttcggg
      721 ttccgcattg tcgaggtgcc cgtcccgacg cgctacttcc ccgaagcctc ctccgtcaac
      781 ttccgccgca gcgtcaccta tgggttggcg acgctgaacg tggcccggcg ctacctcttc
      841 cacaaactgg gcatccgccg ctacaagcaa ttcagcaagc ggctgaagga tgtgctggcg
      901 aagcagcacc agggaaccat aatccgccag ccctaacagc ggcgcaggcc cgcccggtgg
      961 gcggcggcta tcggagagaa agctttgtta cgcgataaaa agttctggat cggggtagcg
     1021 atcagtatca tcttcctata cctgaccttc cgcggccagg acttcgggaa aattggagaa
     1081 tccctgagcc gggttcacta ctgggccttg atacctgccc tcgcctgcta ctttggtggc
     1141 gtctggatcc gggcgctccg ctggggagtg ctgctctcgc ccgtccgcaa aggcattcac
     1201 ccgaatcgct tgtttcgctt cctcgccatc ggctatatgg gcaacgatat tttgcccgcc
     1261 cgcctcggcg atgtcatccg cgtctatata ctgagccgcc gcgagagcgt gaccaaaagt
     1321 gcgacgctgg cgacgatcct ggtggagcgc attttcgacg gcctgaccat gatcggcttc
     1381 ctggccggat cggcgctgtt tatacagatc aacgcggatc tgacgctggc cctgcgcgtg
     1441 tcgacgatct tgttcctggc cggcctgctg gtattcgtct tcctggcggc agcaccccag
     1501 cggatcgtca cgctgatccg cctcattctt gggcgcagcc ccgtcggcaa ggtgatacct
     1561 gagtcgctgc acgaacgcgc gttgcacatg acgacttcct ttgtcgaggg tcttagcgtg
     1621 ctgcgcaatt ggcgcgggtt attaagcgtc atgggcctat ccgtcgccgc ctggagtgcc
     1681 gaggccgcca tgtactacat catcggcgcc tggggcttcg acctgcaaat accggtgcat
     1741 gcatacacca tgacgaccgc ggcggccaat ctaggcacgc tggttccgtc gtcgcccggc
     1801 tatatcggcg tgttcgaggg tatcgctatt cctgtgctga ctaatgtgtt cagtctgggc
     1861 ccgcatatcg ccgccagcta cgtcgtgctc ctgcacgccg cgctctactt cccggtgact
     1921 ctcctgggcc tcttctacat ggtgcgcgag agtgtgtcct ggagcgaact ggcggccctg
     1981 gagaagaaag aggccgccgg ccaacccgcc gtcgaggagg acgattccgg cgacaatgcc
     2041 ggcggttcgg gcggccccgg cgatatgctg agcgtgtccg tgggcggggt gcccgccgcc
     2101 ggtgtgcgga ccggcgcgcg cgatgccgcg cagtccggca agcgttaagc cggcgccggc
     2161 aatcattgcg accttgtaag acacaccggg cgatcttaaa cccgccttta acccccacct
     2221 gatagagtgg gtccggtgcc gcgcgagaat agcggcgccg cccctgcccg gccaggcagg
     2281 ccgggaatcc gggtagcccc gtgggctgcc gtgctactga cccatctgtg aaggagtatg
     2341 tatccgttgg acctgatcca ggctatcgtg ctcggcctcg tgcaagggct ggccgagttt
     2401 atccccatta gttcgtccgc acacctgatt attattccct ggctcttcgg ctggaaagac
     2461 cccggcctcg ccttcgacgt ggcgctgcat atgggcacgc tcatcgccgt gctggtttat
     2521 ttctggcagg actgggtgcg cctgatccgc ggcgcgctca acagtctcgc ccagcgcaac
     2581 ctgaccgccg atcccgacgg caagctcgcc tggtacatcg ttctggcgac catccccggt
     2641 gcgctggccg gggcactggg cgagagcaaa ctcgacgaga tcttccacgg cgatacgccc
     2701 gccgctgccg ctaacggcat cctgatcatt ggcgtggtga tgattgccct ggccgcgctg
     2761 ctctggatcg ccgagcgaat cgccagccac cgctacgata tgagccacct aaccctccag
     2821 caagcgctca ccgtcggcgt ggcccaggca ttcgctatca tccccggcgt ctcgcggtcg
     2881 ggcagcacaa tcaccgccgg gctgtttatg aacctgaagc gagaagcggc ggcgcgattc
     2941 agtttcctgc tgggtacgcc ggtcattgtc ggcgcggggg tcaagaaagc ctacgacatc
     3001 ctgaaagagg gtggcatccc ctccggtgac ctacccgctt tcattgtcgg cttcctggtc
     3061 gcggcgatca gcggctacgc ggccatcttc ctgctgctgc gcttcctgca gcgcaacacc
     3121 acgctgccct tcatcgttta ccgggtggtg gtgggcgcgc tgctgatcgt gttcgtgctg
     3181 gccgggttcc gccgctagag agagggcctg cttgcggagg accgagtgcg cgccggtatg
     3241 gtagaggcga cccccgccgc ccgcgccggg atcggcgggg ccgcgctggc actggcgctg
     3301 ctcggtcagg cgctgctcga ccggcgcgcg accttcggcg agacgaccgg ctggctcgtg
     3361 ctggctggcg cggccagcct ggccgtctgg gcgctggggc cgcgcgcgct acccgccccg
     3421 cccgcccagg ccgcctccgc gaccgagcgc gccgggcggc tgcggctgct gatcctggcc
     3481 ggggccgtca tctgtggtct ggccgcgctg cccggcttca tacaactggc gcaaaccggc
     3541 gggcaggcag ccccgcgggc gggcttgccc tggctggcct ggctggtgag catcggcctc
     3601 ttcatcgtcg ccgccctggt agtcccccac cctaattccg ctgggcgaca ggccggtttg
     3661 aaaccggccc ctacaccgca aaatcaaaac ggcgaactac tgatcgcgcg cccggcgcaa
     3721 ccgccgccgg attccgcgac cccggcaccc gctccacctc tcagcgcgcg gctgcgagcg
     3781 gccctgccct ggatcgccat catcggcgcg gcggccttcc tgcggctgtg gcagcttgac
     3841 agcgtgccgc gcggcctctg ggccgacgag gcgatagcag ggctacaggc agaggacatc
     3901 ctctatcacg gctggcgccc gctgttcatc ggcggggcca acggtgaacc ggccctctac
     3961 ctctacggca tcgccgtcct gatcaaattc ctcggcccca ccgtcgtcgc ggtgcgcctg
     4021 cccatggccc tggccggcac gctggccgtc gcggcgctcg gctggctggc cgccccgctc
     4081 tacgggcggc gggtggccct ggtcgcggcg gcgctgctgg cggccatgac ctggcagatc
     4141 aacttcagcc gcatcggctt caacgccgcc tggtcgctgc cggtcgatct gctggcggcg
     4201 ggctgcctgc tgcgcgccct ggcgacgggc cggctgcgct ggtacgcggg cgcgggcctg
     4261 ttcttcggcc tcggcttcca gctttactat atttcgcgcg cctggctgct gctgctggcg
     4321 gggttgctgg tctaccggct gctgaccgag cggggcctgt ggcggggggc gcgcgcgggg
     4381 ctggcctgga ccggcctggg cctgctgctg gcggcggccc cggtgctggt ctacgcggtg
     4441 atccagcccg ccgactacgc ggcgcgcgct gcctcggtgt ccatcgccga cgcgatccag
     4501 ggcggcggca gcctggcccc gctgggcgaa acgctgctca agcacctgcg catgtggaac
     4561 gtcatgggcg acttcaacgg gcggcacaat ctgcccgatg cccgcctgct cgacggcgtg
     4621 accgccgcgc tgctgcccgt ggggctggcc ctcgccgtcg ccgccacgtg ggcgcgccgc
     4681 cgcgggtggc ggatcgactg gcgcttcctg ttcccggtgg ccgggctggt cgtgctgctg
     4741 gcgggcgggg tgctgtccaa ccccggcgac gcgccgcagg cgttgcgcac gctgggcgac
     4801 accagcatgg tcgccttgct ggctgcgctg cccctcgccg cctggtgggc cacgctcgac
     4861 ggcatcacct ggcgcccggc gcggagggta gcggtggggg cgctggccgt gctcggcgtg
     4921 ctgctggtgg tctttgtcct gaactaccgg cgctatttct tcgtgcagga gccgaatccc
     4981 gccgtctaca aagagttctc gacgcccgcc accctggtct acgagatggt cgccgcgcag
     5041 gggccggaca ccgattatta cctctcgcgc gggctctacg agcaggcgac catcgaatac
     5101 ctggccgggg gccgcatcgg cacgctgttc agcgggctgc acgagacgcc cctcccgccc
     5161 ggcgcgccgg gccgcccggc tatgctcttc ctgaacaacc gcgacagtgg cgcgctcgac
     5221 tggctgcgca cgctctatcc gaacgcgaaa atcgacagcg tgaccgggcc gcagggcggc
     5281 aacgcgcttt ttttcgccgt caccgtgccg cccgc
//
LOCUS       DASUYC010000647         2604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1964837, whole genome shotgun sequence.
ACCESSION   DASUYC010000647 DASUYC010000000
VERSION     DASUYC010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1964837"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..1088
                     /locus_tag="VF276_10520"
     CDS             105..1088
                     /locus_tag="VF276_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6607336.1"
                     /translation="MRAYIALILLLVIFSVLSPAFLTPGTLTILASHVALNAILALGM
                     TFVILTGGIDLSVGSIVGLAAMVAGGLINQGLVLEVFDVTIYFNVWIVILLTLIVGVI
                     VGAVNGWVITRFNVAPFIATLGMLYVARGAALLRSNGATFPNLVGAEDLGNTGYPVLG
                     AGIFLGLPIPIWLMIIFGVASAFIAYKTPLGRQVFAIGGNERAAELAGVRVNRVKMLV
                     YMISGFCAAMVGLIISSQLVAAHPATGETFELSAIAAVVLGGTSLAGGRGSIGGTIIG
                     AFVIGVLSDGLVLLGVSEFWQTVIKGVVIILAVVVDQLQQRLQSRAVLQRA"
     gene            1415..2251
                     /locus_tag="VF276_10525"
                     /pseudo
     CDS             1415..2251
                     /locus_tag="VF276_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015917646.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose ABC transporter substrate-binding
                     protein"
     gene            2351..2604
                     /locus_tag="VF276_10530"
                     /pseudo
     CDS             2351..2604
                     /locus_tag="VF276_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014763055.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
BASE COUNT          487 a          830 c          823 g          464 t
ORIGIN      
        1 caatggggat taacgtccag gtaccttcca gcccaggcgc aggcgccccc ggcagtgccg
       61 tgatccagcg acagcggatc tcgccggttt cgctcctgct ccggatgcgc gcctatatcg
      121 cgctgatcct gctgctcgtc atcttctcgg tgttgtcgcc cgccttcctg acaccgggca
      181 ccttgactat cctggcctca cacgtcgcgc tcaatgccat cctggcgctc ggcatgacct
      241 tcgtcattct gaccggaggc attgacctgt ccgttggctc gattgtgggt ctggccgcga
      301 tggtggcggg cggtttaatc aaccagggcc tggtgctcga agtgttcgac gtcacgatct
      361 acttcaacgt ctggatcgtg atcctgctca cgctcattgt cggcgtgatc gtcggcgcgg
      421 tcaacgggtg ggtgatcacc cggttcaatg tcgccccgtt catcgccacc ctgggcatgc
      481 tgtatgtcgc acggggcgcc gcactcctgc gctcgaacgg cgcgactttc cccaacctgg
      541 tgggcgcgga ggatctcggc aacaccggat atccggtgct gggcgctgga atattcctgg
      601 ggctgccgat accgatctgg ctgatgatta tcttcggcgt tgcgtccgcg ttcatcgcct
      661 ataagacgcc gctcgggcgg caagtgtttg ccatcggtgg caacgagcgc gcggccgaac
      721 tggccggcgt gcgcgtgaat cgcgtgaaga tgctcgtcta tatgatctcc ggcttctgcg
      781 ccgccatggt gggcctgatt atctcgtcgc aactcgtagc cgcgcatccc gccaccgggg
      841 agacattcga gttgagcgcg attgcggcgg tcgtgctcgg tggtacgtcg ctcgcgggcg
      901 gccgcggatc tatcggcggc acgatcatcg gcgcctttgt tatcggcgtt ctgagcgacg
      961 ggctggtcct gctgggtgtg tccgagttct ggcagacggt tatcaagggg gtcgttatca
     1021 ttctggccgt cgttgtcgac cagctccaac agagactgca aagtcgcgcc gtgctccaac
     1081 gcgcgtaaga aaggaggttc ccggagccgc agttgcgaca tcctggcaga ggtttttcta
     1141 aaagcgtttt gaccagataa cacaaggagg aagttccgtg tccatgcaca aacaaccgcg
     1201 cattcttgcg ctgctgatga tagtaaccat cctcaccatg ctgctctctg cgtgcggtga
     1261 ggccgccccg acggcgacgg cggtgcctgc acaagcgact actaccacgg cggtatcgcc
     1321 tgaagccacc gccaccaccc cggcggaggc gaccgccacg acggcagccc cggctaccag
     1381 cacaacgggc agcggcgcga gcggcggcgg caagctgatc gcgatcatta cgccgtcgca
     1441 tgacaacccc ttcttcaagg ccgaggctga cgccgctgat gcccacgcca aagaactggg
     1501 ctacgatacc ctgatcctgg tgcacgacga cgacgccaac aagcagaacc aactcttcga
     1561 taccgccatc tcgcgcaagg ccgccgcgat catcctcgac aacgcgggcg ccgacgcctc
     1621 gatctcggcg gtgcagaagg ccaaggacgc cggtattccc agcttcctga ttgaccgcga
     1681 gattaaccag acgggggtgg ccgtcgccca gatcgtgtcg gacaactacc agggagcgac
     1741 cctgggcgcg caggagttcg tgcggctgat gggtgagaag ggcaccttcg tcgaactcac
     1801 gggcaaggag tcggacacga acgcggggat tcgctccaag ggctaccacg acatcatcga
     1861 caagtacccg gacatgaaga tggtggccca gcagagcgcc aactggagcc agaccgaggc
     1921 gttccaaaag atgcagacga tcctgcaagc gaacccggat atcaaggggg tcatctcggg
     1981 caacgacacg atggcgctgg gggcgtcggc ggccttgaag gcggcgggca agaacgacgt
     2041 gatggtggtc ggctttgacg gcagcccgga cgtgatcgcc gagatcaagg cgggcggcaa
     2101 catcaaggcg acggtgctgc agccggcggc gggcatcgcc gagatggcgg tggaccaggc
     2161 ggacgcctat ctgaaggccg ggaacaaggc cagccagccg gagaagcaat cggtggactg
     2221 cgtattggtc acgcctcaga atgccgatca gttcggtgtc ttcggcccca agtctagcgc
     2281 gccgggcgat acgacgccga gcgccagccc cactgctggc ggcagcagtt cgagcagcgg
     2341 cagcggcaag ctgatcgcga tcattacgcc gtcgcatgac aaccccttct tcaaggccga
     2401 ggccgacgcc gccgacgctc atgccaagga actgggctac gataccctga tcctggtgca
     2461 cgacgacgac gccaacaagc agaaccagct cttcgatacc gccatctcgc gcaaggccgc
     2521 cgcgatcatc ctcgacaacg cgggcgccga cgcctcgatc tcggcggtgc agaaggccaa
     2581 ggacgccggt attcccagct tcct
//
LOCUS       DASUYC010000648         3555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1016503, whole genome shotgun sequence.
ACCESSION   DASUYC010000648 DASUYC010000000
VERSION     DASUYC010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016503"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1052
                     /locus_tag="VF276_10535"
     CDS             <1..1052
                     /locus_tag="VF276_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX6607337.1"
                     /translation="FLSGADQIPVPQERRKGNGHALVVHDANENNLKNIDVTFPLGKF
                     VCVTGVSGSGKSTLVTDILYRELARKLNRAKEKPGSHGQVAGIEHLDKVIEIDQSPIG
                     RTPRSNPATYTGMFTAIREVFAKVPEAKLRGYTSGRFSFNVKGGRCEACRGEGIIEIE
                     MQFLPAVYVPCEVCHGQRYNREALEIRYKGKSIADVLNMTVEEALEFFAHIPAIRSKL
                     ATLNDVGLGYIHLGQPATTLSGGEAQRVKLATELSRRATGKTIYILDEPTTGLHFADV
                     KRLLDVLQRLTDAGNSVLVIEHNLDVIKTADWLIDLGPEGGDSGGEVIAEGTPEQVAQ
                     VAASHTGQFLKKMLH"
     gene            complement(1119..1937)
                     /locus_tag="VF276_10540"
     CDS             complement(1119..1937)
                     /locus_tag="VF276_10540"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6607338.1"
                     /translation="MADDPQIVYTLPGMDTVPVRKDIVYKSVDGESLMLDVYTPPGAE
                     PGARRPAVLFVHGDGPPDMLRHAKDWGVFTGWGRLAALSGLVGITFNHRSTAGWTKLP
                     DVAGDIADLIAFVRREAPDLGLDADRLGVLVASAGGPAGLAPLLRDPPPFLRALVAYY
                     TVMDLQGLHAHVELPPHVTPDLLREFSPVAQVPAGPESCPPLLIARAGLDRATINASI
                     DAFIQAALAHNLDLDVMNHPTGRHGFDVLDADARSREIIRRTLAFLTQHLDPAA"
     gene            complement(1961..2236)
                     /locus_tag="VF276_10545"
     CDS             complement(1961..2236)
                     /locus_tag="VF276_10545"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEX6607339.1"
                     /translation="MANTDTNGARPPIVVYSGPLCSGCDEVKAYLAAHDLAYEERNIR
                     GNIETMIEFRRKGYDILPVIEVGDQVISEYESVAQVEAALLAAGYLS"
     gene            complement(2609..>3555)
                     /locus_tag="VF276_10550"
     CDS             complement(2609..>3555)
                     /locus_tag="VF276_10550"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004893138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEX6607340.1"
                     /translation="LMFKTFVGPVEEDASVAYLRPETAQGIFVNFLNVQQSMRRKLPF
                     GIAQQGKSFRNEITPGNFIFRTREFEQMELEYFVRPGEDEIWYPRWREERFNWYLRLG
                     VNPERLRLRDHEQSELSHYSKATADVEYLYPWGWGELEGIANRTDYDLRQHMEHSGKD
                     LRYYDEESGTRFLPYVIEPAVGVDRASLVFLFDAYAEEQVGDKNDTRVVLHLHPELAP
                     IKVAVMPLSKKEPLTNLAHDIHRSIRKHWMTQYDETGGAIGKRYRRQDEIGTPLCVTV
                     DFDSLEDHAVTIRERDSMAQVRVPIAELVPALREKLGF"
BASE COUNT          606 a         1181 c         1186 g          582 t
ORIGIN      
        1 gcttcctgtc gggcgccgac cagattcccg tgcctcaaga gcggcgcaag ggcaacggcc
       61 acgcgctggt ggtgcatgac gccaacgaga acaacctgaa gaacatcgac gtgacgttcc
      121 cgttgggcaa gttcgtctgc gtcaccggcg tctcggggtc gggcaagtcc acgctcgtca
      181 ccgacatcct gtaccgcgaa ctggcgcgca agctgaaccg ggccaaggag aagccgggca
      241 gccacggcca ggtggcgggc atcgaacacc tggacaaggt gatcgagatc gaccagtcgc
      301 ccatcgggcg cacgccgcgc tcgaacccgg cgacgtacac cggcatgttt acggccatcc
      361 gcgaggtgtt cgccaaggtg cccgaagcca agctgcgcgg ctataccagc gggcgcttct
      421 cgttcaacgt gaagggcggg cgctgcgaag cctgccgcgg cgagggcatc atcgagatcg
      481 agatgcagtt cctgcccgcg gtctacgtac cctgcgaggt ctgccacggc cagcgctaca
      541 accgcgaagc cctggagatc cgctacaagg gcaagagcat cgccgacgtg ctcaacatga
      601 cggtggagga agcgctggag ttcttcgcgc acatcccggc catccgcagc aagctggcga
      661 cgttgaacga cgtgggcctg ggctacatcc acctgggcca gccggccacc acgctgtccg
      721 gtggcgaggc ccagcgcgtg aaactggcga ccgagctgtc gcggcgggcc accggcaaga
      781 cgatctacat cctggacgag ccgaccaccg gcctgcactt cgccgatgtg aagcggctgc
      841 tcgatgtctt gcagcggctg accgacgcag gcaactcggt gctggtgatc gagcacaacc
      901 tggacgtgat caagacggcg gactggctca tcgacctggg gcccgaaggc ggcgacagcg
      961 gcggcgaggt catcgccgag ggcacgcccg aacaggtggc ccaggtggca gccagccaca
     1021 ccgggcagtt cctgaagaag atgctgcact gacgcctaca agcgcgcggg gccggtcgct
     1081 ggaccggccc cgcgttttgc tttcgggtgc gcctccagtc atgcggcggg atcgaggtgc
     1141 tgcgtgagga acgccagggt ccgccggatg atctcgcgag accgggcatc ggcgtcgagc
     1201 acgtcgaagc cgtgccggcc cgtggggtga ttcatcacat ccaggtcgag gttatgggcc
     1261 agcgccgcct ggataaaggc atcaatcgac gcattgatcg tggcgcgatc cagccccgcg
     1321 cgggcgatca gcagcggcgg gcacgactcg ggcccggcgg gcacctgggc caccggcgaa
     1381 aactcgcgca gcagatcggg tgtgacgtgc gggggcaact cgacgtgagc gtgcagcccc
     1441 tgtaggtcca tgaccgtgta gtacgcgacc agggcgcgca ggaacggcgg cgggtcgcgc
     1501 aacagcgggg ccagcccggc ggggccgcct gccgaggcga ccaagacccc gagccgatcc
     1561 gcgtcgaggc cgagatccgg cgcttcccgc cgcacaaagg cgatcaggtc ggcgatgtcg
     1621 ccggccacgt ccggcagctt ggtccagccc gccgtcgagc ggtgattaaa ggtgataccc
     1681 accaggcccg acagggcggc cagccggccc cagccggtga acacgcccca atccttggcg
     1741 tggcgcagca tgtcgggcgg cccatcgccg tggacgaaca gcacggctgg tcgccgcgcg
     1801 ccgggttccg cccccggtgg cgtgtagaca tcgagcatga gcgactcgcc gtccacgctc
     1861 ttgtacacga tgtctttgcg caccggcacg gtgtccatgc ccggcagcgt gtagacaatc
     1921 tgcggatcgt cggccattac ttctgcctcc ttgccgcctt ttagctcagg tagcccgccg
     1981 ccagcagggc cgcctctacc tgcgccaccg actcgtactc gctgatcacc tggtcgccca
     2041 cctcgatcac cggcaggatg tcgtagccct tgcgccggaa ctcgatcatg gtctcgatat
     2101 tgccacggat gttgcgctcc tcgtaggcca ggtcgtgcgc ggccaggtac gccttgacct
     2161 cgtcgcagcc gctgcacagc ggcccgctgt agacgacgat agggggacgc gcaccgttgg
     2221 tatccgtgtt agccatagtt gttgctcctt gctcgcacca gacgagactc ttggcggcat
     2281 tacgctgaaa tcgggccggg tgcgtggggc ggcacggcgg gtttgaaacc cgcccctaca
     2341 ccgccatccc ggcctttgtc agcgcacatg gcgggccgta aatacagagt tggcgcagca
     2401 tgatcaggct tgcaggagac gagactcttc gctccgctca gagtgacaag atcctgcagg
     2461 ccatgcgctc gctgtagagg ccggtttcaa accggcccgc ccctccatcg cgctgtaggc
     2521 gccggtttga aaccggcccg ctactccgtt tgacaaaaag agagcgcccg cacgaaacgt
     2581 gccgggcgcc ctcccttgcg ggtcggggct agaagccgag cttttcgcgc agcgcgggca
     2641 cgagttccgc aatcggcacg cggacctggg ccatggagtc gcgctcgcgg atggtcaccg
     2701 cgtggtcctc caggctgtcg aagtccaccg tcacgcagag cggtgtaccg atctcgtcct
     2761 ggcggcggta gcgcttgccg atggcgccgc ccgtctcgtc gtactgcgtc atccagtgct
     2821 tgcggatgct gcggtggatg tcgtgagcca ggttggtcag cggctccttc ttggacagcg
     2881 gcatcaccgc caccttgatc ggggccagtt cggggtgcag gtgcagcacc acgcgcgtgt
     2941 cgttcttgtc gcccacctgc tcctcggcgt aggcgtcgaa caggaagacc agcgacgccc
     3001 ggtccacgcc caccgccggc tcgatcacat agggcaggaa gcgcgtgccc gactcctcgt
     3061 cgtagtagcg caggtccttg ccgctatgct ccatgtgctg gcgcaggtcg tagtcggtgc
     3121 ggttggcgat gccttcgagt tcgccccagc cccacgggta gaggtactcc acgtcggcgg
     3181 tggccttcga gtagtgcgac agttccgact gttcgtggtc gcgcaggcgc agccgctccg
     3241 ggttcacgcc gagccgcaga taccagttga agcgctcctc gcgccagcgc ggataccaga
     3301 tctcgtcctc gccgggccgc acgaagtact ccagctccat ctgctcgaac tcgcgcgtgc
     3361 ggaagatgaa gttgccgggc gtgatctcgt tgcggaacga tttaccctgc tgtgcgatgc
     3421 cgaagggcag cttgcggcgc atcgactgtt gcacgttcag gaagttgacg aagatgccct
     3481 gggccgtctc ggggcgcaga taagccaccg atgcgtcttc ctccaccggg ccgacgaagg
     3541 tcttgaacat caggt
//
LOCUS       DASUYC010000649         2030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1144069, whole genome shotgun sequence.
ACCESSION   DASUYC010000649 DASUYC010000000
VERSION     DASUYC010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1144069"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(209..1204)
                     /locus_tag="VF276_10555"
     CDS             complement(209..1204)
                     /locus_tag="VF276_10555"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-heptose synthase"
                     /protein_id="HEX6607341.1"
                     /translation="MNRLLALLPAFAGKRIVVLGDLILDEYIIGGPSRVSREAPIVIL
                     EFNRRTVLPGGGTAPASNIQSLGAQAIQAGVIGADKAGDELRAALAARGVDTRGLVVD
                     PGRPTILKSRIVAEGPGRLQQQIARVDTLDRRPIAGDVENAVIATIERELPHADALLI
                     SDYQSGTVSPAVVDAARRVAARTGALITVDAQGDFAKFQGVTIFRCNDREAESALHTE
                     LRGEAAFADGLRRLRAELGTEGVVVTRGGEGMSILDQQDHYHHIPVSNSAEVFDVTGA
                     GDTVIAVLTLALTAGADLLDAARLANYGAGIVVQKLGNVPLNPLELRARLLQTAE"
     gene            complement(1208..1864)
                     /locus_tag="VF276_10560"
     CDS             complement(1208..1864)
                     /locus_tag="VF276_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607342.1"
                     /translation="MAYNRGPYDGGTTTGEPESGTLYCANHPTVPTVLRCARCEKPIC
                     ARCRVHTPVGYRCLQCANLSVLPTYAMDSTSVTKSVLAGFAFTTVVGLVWALLPGYDF
                     WAALILGIATGEVVSGAANQKRGPALQAVGLAAAVWGIVLSRAALAFFLTVPPLLNFI
                     AGFPGNNASNLLRGGAALVGLISRGDYGGILLSLVNRTDLLGLVFVLMALVLTFIRLR
                     "
BASE COUNT          337 a          677 c          710 g          306 t
ORIGIN      
        1 gacagggtat ttggttgaag cgcgatgccg aaaccacagc ttggacaagt cgattgtcca
       61 ggcaccaaac gcatctggat tttgcaacgc ggacaggtgt aatcgaggga caactggtct
      121 ccaatccttc ctgactctca gccgacacac atgacgaatt gcagagcgta gttaagcccg
      181 acagcatcgc gccgtttccg gtcgccggtt actccgccgt ttgcagcagg cgggcgcgca
      241 gttccagcgg gttgagcggc acgttgccca gcttctggac cacgatgccc gcgccgtagt
      301 tcgccagccg ggcggcgtcg agcagatcgg cgcccgccgt cagggccagc gtgagcacgg
      361 caatgaccgt gtcgcccgcg ccggtcacgt cgaaaacctc ggcgctgttg ctgaccggga
      421 tgtggtgata gtggtcctgc tggtcgagga tggacatgcc ctccccgccg cgggtgacga
      481 ccacgccctc ggtgcccagt tcggcgcgca ggcggcgcag gccgtcggca aacgccgcct
      541 cgccgcggag ctcggtgtgc agcgcgctct cggcctcgcg gtcgttgcag cggaagatgg
      601 tgacgccctg gaacttggcg aaatcgccct gggcgtccac ggtgatcagg gcgccggtgc
      661 gcgcggccac gcgccgggcg gcatcaacta ccgccgggct gaccgtgccg ctctggtaat
      721 cgctgatcag cagggcatcg gcgtggggca attcgcgctc gatcgtggcg atcacggcgt
      781 tctccacgtc cccggcgatg ggccgccggt cgagagtatc gacgcgggca atctgctgtt
      841 gcaggcgccc cggcccttcg gccacgatgc gcgacttgag gatggtgggc cggccaggat
      901 cgactacgag gccgcgcgtg tccacacccc gcgcggccag ggcggcgcgc aactcgtcgc
      961 cggccttgtc cgcgccgatc acgccggcct ggatggcctg ggcgcccagc gactggatgt
     1021 tcgacgcggg cgccgtgccg ccgccgggca gcactgtgcg ccggttaaac tccaggatga
     1081 cgatgggcgc ctcgcgcgac acccggctgg gcccgccgat gatgtactcg tcgaggatga
     1141 ggtcgcccag gacgacgatg cgcttgccgg cgaaggcggg cagcagggcg agtaagcggt
     1201 tcatgcgtca gcgcagccgg ataaaggtga ggaccaacgc catcaacaca aataccaggc
     1261 ccagcaaatc ggtgcggttc accagactca gcaggatgcc gccgtaatcc ccccggctga
     1321 tgagcccgac cagtgccgca ccgccgcgca gcaggttcga cgcgttgttg ccggggaagc
     1381 cggcgataaa gttcaacagc ggcggcacgg tgaggaaaaa ggccagcgcg gcgcggctca
     1441 ggacaatgcc ccagaccgcc gccgccagcc cgacggcttg cagggccggg ccgcgcttct
     1501 ggttggccgc cccgctcacc acctcgcccg tggcgatgcc gagaatgagc gccgcccaga
     1561 agtcatagcc gggcagcagc gcccacacca gtccgacgac ggtggtgaag gcgaagcccg
     1621 ccagcaccga cttcgtcacc gaggtggagt ccatcgcata ggtgggcagc acgctgaggt
     1681 tggcgcattg caggcagcga tagcccaccg gcgtgtggac ccggcagcgg gcgcagatgg
     1741 gcttctcgca gcgggcacag cgcagcaccg tgggcaccgt gggatgattg gcgcagtaca
     1801 gcgtgcccga ttcgggctcg ccggtggtcg tgccgccgtc gtaggggccg cgattatacg
     1861 ccacgtcagg cccccatccg gggcgcggca cccagcacgc ggcgcgcgcc cagatatgta
     1921 tcgcggcggc catggtattc ggggtgcgtg gggtcgaggc tctgcaccag cacctgtttg
     1981 gcgctgccgc tggcgtggat catctgcccc tcgccgatat acatggtgac
//
LOCUS       DASUYC010000650         2412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_42832,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000650 DASUYC010000000
VERSION     DASUYC010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42832"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1860)
                     /locus_tag="VF276_10565"
     CDS             complement(<1..1860)
                     /locus_tag="VF276_10565"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX6607343.1"
                     /translation="MNYLRIIRKHVAIATSIIWILVLVLPAFGSAMAQETRGAPGRNA
                     PAALAAASGPTDPAELEAFLDDLFARQMQEHHIAGAAVSVVKDGKLFFAKGYGYADVA
                     EGVPVDPAQTIFRIGSVGKLFTWTAVLQLVEQGKLDLDADINTYLDFRIPATYPQPIT
                     LKHLLTHTSGFEDRQLGSVVADARDLVPVRDWLVSHMSARVHPPGYAGYANYNAILAG
                     YIVARVSGQPYDQYIQDHILNPLGMTHSSVRSPLPPDLRPLASVGYTYADGGFQAFPE
                     YIAQPAGLPSGMLQASVTDMARFMIAHLEGGRYSDGNIAEARILKEATAQEMQGALYT
                     PDPRLRGTAYGFFDWSDNGQQTLGHTGYAPPMHSVLLLLPDQHLGVFVAFNSKDVGAL
                     TTQHSGFQRAFFDHYYPAPAGAPVQPPADFAARAGQFVGSYNAYSAYTTLIKISGLFG
                     GGYTVDISNPGDGTLLLTLEGFKLRIAEVAPLYFRQVDGPFSVLFRTDASGRITDLVT
                     DLMPEYAAAKLAWYETSGFNMALLLGCVLIFLAMIPVAVILLIRDRRRSDRNPAPRGA
                     RAALWIILGISLLNLLLVGGMMWGSMGGMSNELLDPPWIIKILLGGGVIAAVLT"
     gene            complement(2145..>2412)
                     /locus_tag="VF276_10570"
     CDS             complement(2145..>2412)
                     /locus_tag="VF276_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006542892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX6607344.1"
                     /translation="DTPLSQPQPRGARRRPPGPPDALTPREWQVARLAAGHCTDRQIA
                     EALTITEGTAGLHVHHILQKLGLRSRAQIADWAQATGLIDTGKA"
BASE COUNT          449 a          729 c          811 g          423 t
ORIGIN      
        1 cgtcagcacg gccgcgatca cccccccgcc gagcaggatc ttgatgatcc atggtgggtc
       61 gagcaactcg ttggacatgc cccccatcga cccccacatc atgccgccca ccagcagcag
      121 gttcagcagg ctgatcccca ggatgatcca cagggccgcg cgagcgccgc gcggggcggg
      181 gttccggtcg ctgcggcggc ggtctcgaat caagaggatc actgctacgg ggatcatggc
      241 caggaagatc aagacgcacc ccaggagcaa ggccatgttg aaaccggacg tttcgtacca
      301 ggccagcttt gcagcagcat actcgggcat gaggtcggta accaagtcgg tgatgcgtcc
      361 gctggcgtcc gtgcgaaaca gcaccgagaa ggggccgtct acctgccgga agtacagcgg
      421 cgccacttcg gcaatgcgga gcttgaaccc ctccagggtg aggaggagcg taccgtcacc
      481 cggattgctg atgtccacgg tgtagccgcc gccgaagagt ccactgatct tgatcagggt
      541 ggtatatgcg ctgtaggcgt tatagctgcc gacaaactga ccggcgcgcg ccgcgaagtc
      601 cgcaggaggc tggacgggcg cgccggccgg cgcgggatag tagtggtcga aaaacgcgcg
      661 ctggaacccg gaatgctgcg tggtcagcgc ccccacatcc ttgctgttga aggccacaaa
      721 tacgcccagg tgctggtcgg gcaagaggag caggacacta tgcatcggtg gcgcatagcc
      781 ggtatggccg agcgtctgct ggccgttgtc gctccaatcg aaaaagccgt aagcagtgcc
      841 ccggaggcgc gggtcaggcg tgtacaacgc accctgcatt tcctgcgcgg tggcttcttt
      901 cagaatgcgg gcctcggcga tgttgccatc gctgtagcgg ccgccctcca ggtgcgcgat
      961 catgaaccgg gccatgtcgg tgacactggc ctggagcatg ccggacggca ggcccgccgg
     1021 ctgggcaatg tattcaggga atgcctggaa tccgccatcg gcataggtgt atcccacgga
     1081 tgccagtggg cgcaggtccg gcggtagcgg cgaccggaca gatgagtgcg tcatacccaa
     1141 tgggttgagg atatggtcct gaatgtactg atcatagggc tggcccgaga cccgcgccac
     1201 gatgtatccg gccaatatgg cattgtagtt tgcgtaaccc gcataaccgg gcggatgcac
     1261 ccgcgccgac atatgggaga ccagccagtc gcgcacgggc accaggtccc tggcatccgc
     1321 gaccacactc cccaactgcc ggtcttcgaa cccggacgta tgggtcaaca ggtgcttgag
     1381 ggtgatcggt tggggatagg tagccgggat acgaaagtcg agataggtgt tgatatccgc
     1441 gtccaggtcg agcttaccct gctcgacgag ctgcaacacc gccgtccagg tgaacagctt
     1501 gccgacggag ccaatgcgaa agatcgtctg tgcgggatca acagggacgc cttcggccac
     1561 atcggcgtag ccgtagcctt tggcgaaaaa cagcttgccg tctttgacga cggagacggc
     1621 ggcgccggcg atgtggtgct cttgcatctg ccgggcgaag agatcatcca ggaaggcttc
     1681 cagttcggcg ggatcggtcg ggcctgaggc cgctgcgagc gccgcgggcg cattccggcc
     1741 cggcgcgccc ctggtttcct gggccatggc cgacccaaag gccggcagca ctagcacgag
     1801 aatccagata atcgacgtag cgatcgccac gtgtttccgg ataatacgca ggtaattcat
     1861 gggcttgctc cttatccata ggcgatagcg acgaaggctc gtactccata cccatcgacc
     1921 agccggtctg tttggttgcc ggcagcaccc atgcaactgc cttcccataa ggcggcgccg
     1981 atcagttgac cgtctccggg ggaagtcgta agtagtccgc tcgcctcatc atagccgggc
     2041 ccgatgcatt tgccatcgca cgacgttata ggcgcgcgct atatctttcg cgctccgtca
     2101 gcagaggcgt gggtaacact atatggatcc ggtttaagag aggatcaggc tttcccggtg
     2161 tcgatcagcc cggtggcctg ggcccagtcg gcgatctgcg cgcgcgagcg cagtcccagc
     2221 ttttggagga tgtggtgcac gtgcaggccg gcggtgccct cggtgatggt gagcgcctcg
     2281 gcgatctggc ggtcggtgca gtggcccgcc gccagccggg ctacctgcca ctcgcgcggc
     2341 gtgagggcgt cgggggggcc gggaggtcgg cgccgcgcgc cgcgcggctg cggttgggaa
     2401 aggggcgtat cg
//
LOCUS       DASUYC010000651         6314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1017565, whole genome shotgun sequence.
ACCESSION   DASUYC010000651 DASUYC010000000
VERSION     DASUYC010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6314
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017565"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1188)
                     /locus_tag="VF276_10575"
     CDS             complement(<1..1188)
                     /locus_tag="VF276_10575"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEX6607345.1"
                     /translation="MSVEPTPPAQPEPTPPAPRRSFLLPLVGLLIVLLLIVAGVTLLP
                     KTSETPTPVPPSATAPGPTATELVVAPTGSAVAGLSTGTAVAGGAETPAGLPSAEATT
                     GRGVLTGTTPAGTPGTPGTAGTPGTPGTAGTPGTPGTETAAGTPVAAPTGISVLDSSG
                     TAFVCGLAPGCTQAAVAGMAVTPGGEVRTGPDGQLRLQTPAGLVVLTGTTTLRVTSLD
                     AQQAAITLNSGRVLAQAAPGRTVTLTITTTAGSVAGMGTGFGVAVLPSGGMRVAVPAI
                     GNRAVQVQGSGTAPVLDLAPGQQVTVDATGTAGAAGPLDATEAAALAAMGAFSPAGTP
                     VAAQITELPVPAVSPFPSGAVTATMVLTPPPEQTLPPLTVGPVAGGTGTPVAGGVVTA
                     TVTL"
     gene            complement(1323..1979)
                     /locus_tag="VF276_10580"
     CDS             complement(1323..1979)
                     /locus_tag="VF276_10580"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEX6607346.1"
                     /translation="MRETRRRPRGWVGILGLLACCAAACDNGAATESQPATVLDTRGA
                     TFLCRDSFPADLTTCPAATVGATVVVNGGVHTGPGAATLLMKTRGTTFRLLPNSSLRY
                     VESTSAGALFLLTAGRVFAEHEPGSDNVVIRGGDAGVAPLGTLYTAGIDATGVIISVR
                     EGRVGVSVPPTAPAQPMDAGQGMNVDTGAHAAPTPRPMTPPEAALWRQIGPHLEWSGP
                     "
     gene            complement(1979..3085)
                     /locus_tag="VF276_10585"
     CDS             complement(1979..3085)
                     /locus_tag="VF276_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607347.1"
                     /translation="MNADEAGLRREIIAGLERTIWSPGDGVRDLDRVEAVQLLGRFRD
                     PAAEAALLRVLREGMERIRPAPLGRLLRDTALDTLLRSTLLPRAATNRALARYYNFGG
                     RLLRGRLERNVIYDDIPLLVRHGRGGLVLRAYVLPLVGVLIALVVLAVVLAGAGGLLA
                     GNEFLGAALGLVFFIAFGLGLFNLHQVGLVLLLAAWGRRWPLPWLPRAGGKAATAVVL
                     AVIAFVLGLYAALRLLFLVARGYNDAGGREVAGYLLLLPLLALPCYMLAHDLEVAPPA
                     AGGTARRGPAVALRWASGFIYVIFVLDAYGLTWIQQARAGFRTFGGDLDVVMGMLLWL
                     LYLVLAPLPVLLLLSALGRLTRTRAGGRPAAEAS"
     gene            complement(3082..4197)
                     /locus_tag="VF276_10590"
     CDS             complement(3082..4197)
                     /locus_tag="VF276_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607348.1"
                     /translation="MEQPDPPAAALRQEILAGLARTIWTPPPGVSEFDRVEAVRLLGQ
                     FRDPAAGVALLRVLNEAPRGIGPPLGRLLRDTALDTLLRSSAQPRAVVDKELAVYYDL
                     WRRLRRGRLDRNVIYDDIPLLVQHGRVGVLLRGYVLPLIALLVVIVLGVLFWAGTRSG
                     QTQWADLIIGPVLFVGLGLTVFSLHQIGLVLLVAWFGPRLPLPQLPTRRSKALLGGIL
                     AGAVVLLSLYILALLVDITMSGAPDAGSRPLAVALLALPLLALPCYMLAHDLETGAHT
                     LEGGLAVRGGTFWAAILRWAGGLIYVFFVFGAFSSHSFTRTIQGQSAGVGPVLLGITP
                     YLLYLLLAPVLVLLVLSALGALGRALGRPRAVSGARP"
     gene            complement(4202..5776)
                     /locus_tag="VF276_10595"
     CDS             complement(4202..5776)
                     /locus_tag="VF276_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607349.1"
                     /translation="MSDNPQPAPAVAMTRPQFVMPPSGGTGATYEFFGPTVINAPLSA
                     GNQVIGQDRTADEQQYLFQVASVYTYWRNQYILGPASAQGSPFIPTDTAALFQQQVSA
                     RPGAPAAARQLQRRPIALPDAIRDFWRVLLLGKPGGGKTSYLQYMALQCTRPETTPIP
                     GLLPVVVDLSMYTTTAPMLDFVRNFLRNPPNPQPPEPPVHVQSAWMADSLETYLQNGR
                     ILLMLDGLNELPALPLPAAAARLAQIQAFFRQYTQIRTIVTCRMLDYTTEFDGLDFHT
                     VLLDPWSVDQIADYLQKRQAAGLLDRLRAGDPLLLSLGQVPFLLYMLTELVANFPLPA
                     GNAAAPEFLTSQSGLFKQFVEMLLNWATLKDQENALFFPRPVIIAALAQLAAAMGQAG
                     YRGTSVPHDWAGAHLASDPLALFQGLPPPEGLQGDPRDHLLDFSCAATILDTPATRDT
                     VRFWHLTHQDYFNALAAQQPGVLLGPLTPATDDVTSLAAAMAPQPDAAIAGILQQNDP
                     RAAIVAAKALLSAGGG"
BASE COUNT          980 a         2254 c         2287 g          793 t
ORIGIN      
        1 cagggtcacg gtggccgtga ccacgccgcc cgccaccggg gtgcccgtgc cgccggccac
       61 cgggcccacg gtcagcggcg gcagggtctg ctcaggcggg ggcgtcagca ccatggtggc
      121 cgtgaccgcg ccgctgggga acggcgagac ggcgggcacc ggcaactcgg tgatctgcgc
      181 ggcgacgggc gtaccggcgg gcgagaaggc gcccatggct gccagggccg ccgcctcggt
      241 cgcgtcgagc ggcccggcag ccccggcggt gcccgtggcg tccaccgtga cctgctgacc
      301 gggcgccagg tcgagcacgg gcgcggtgcc cgatccctgg acctgcaccg cacggttgcc
      361 gatggccggg acggcgacgc gcatgccgcc gctgggcagc acggccacgc cgaagccggt
      421 gcccatgccg gccacgctgc cggcggtggt cgtaatcgtg agcgtcacgg tgcggcccgg
      481 cgcggcctgc gccagcactc gcccgctatt cagcgtgatc gccgcctgct gcgcgtccag
      541 gctcgtcacc cgcagcgtgg tggtgccggt cagcacgacc agcccggcgg gcgtttgcag
      601 gcgcaactgg ccgtccggcc cggtgcgcac ctcgccgccc ggcgtcacgg ccatgcccgc
      661 gacggcggcc tgcgtgcagc cgggtgccag cccgcagacg aaggcggtac cgctgctgtc
      721 cagcacgctg atcccggtcg gcgcggcgac cggggtcccc gccgccgtct ccgtgccggg
      781 cgtgcccggt gtaccggcgg tgcccggcgt gcccggcgta ccggcggtgc ccggcgtgcc
      841 cggcgtgccc gccggggtcg tgccggtcag cacgccgcgc ccggtggtcg cctcggcgct
      901 gggcagcccg gcgggcgtct cggcgccccc ggcgacggcg gtgccggtgc tcaggccggc
      961 cacggcgctg cccgtcggcg ccacgacgag ttcggtggcg gtggggccgg gcgcggtcgc
     1021 cgacggcggc acgggcgtgg gggtttcgct ggtcttgggc agcagcgtga ctccggcgac
     1081 gatgagcaag agcacgatca gcaggcccac aagcggcagc aggaacgagc gacgcggcgc
     1141 gggaggcgtg ggttcgggtt gcgcgggagg ggtcggctcc acggacatag ggcattcctc
     1201 gctttcagcg cgccgcgccc cggcggggcg ggcaggcgcg cctatgacta tgtttcccgg
     1261 cgcctcgccg cgtcaccaaa cgggcgccaa tctttagcaa tcaacaagcc ggcaggcggg
     1321 cgtcagggcc cggaccattc gagatgcggg ccgatctggc gccagagcgc cgcttcgggc
     1381 ggcgtcatcg ggcgcggcgt gggcgcggcg tgcgcgccgg tgtccacatt catcccctgc
     1441 ccagcgtcca tcggctgggc cggggccgtg ggcggcaccg atacgccgac ccgcccctcg
     1501 cgcacggaga taatcacccc cgtggcgtcg atcccggcgg tatagagcgt gcccagcgga
     1561 gccaccccgg catcgccgcc gcggatcacc acgttgtcgc tgcccggctc atgctcggcg
     1621 aacacacggc ctgccgtcag caagaatagc gcccccgcac tcgtgctttc aacgtagcgc
     1681 aaggaactat tcggcagcag ccggaacgtc gtgccccgcg tcttcatcag cagggtggcg
     1741 gcgcccggcc ccgtgtgcac gccgccgttg accaccacgg tcgcccccac ggtcgccgcc
     1801 gggcaggtgg tcaggtcggc ggggaagctg tcgcggcaca ggaaagtcgc cccccgcgtg
     1861 tcgagcaccg tcgccggctg ggactcggtc gccgccccat tatcgcaggc ggcggcgcag
     1921 cacgccagca ggcccaggat gcccacccag ccgcggggcc ggcgccgcgt ctcccgcatc
     1981 acgaagcctc cgccgccggg cgcccgcccg cacgggtccg ggttagccgg cccaacgcgc
     2041 tgagcagcag caataccggc agcggcgcca gcaccaggta gagcaaccag agcaacatcc
     2101 ccatcaccac atccagatcg ccgccgaagg tgcggaaccc ggcccgcgct tgctggatcc
     2161 aggtgagccc gtaggcgtcg agcacaaaga tcacgtagat gaacccgctg gcccagcgca
     2221 gggccaccgc cgggccgcgc cgcgcggtcc cgccggcagc agggggcgcc acttcgagat
     2281 cgtgggccag catgtagcag ggcagggcca gcagcggcag cagcagcaag tagcccgcca
     2341 cctcgcggcc acccgcgtca ttatacccgc gcgccaccag gaataacagg cgcagcgcgg
     2401 catacagccc caacacgaag gcgattacgg ccagcacgac ggcggtggcc gccttgccgc
     2461 ccgcgcgggg caaccagggc aagggccagc gccgccccca ggcggccagc agcagcacca
     2521 ggcccacctg gtgcaggttg aacaggccca gcccgaacgc gatgaaaaac accaggccca
     2581 gcgccgcgcc caggaactcg ttcccagcca gcagcccgcc cgcgcccgcc agcacgacgg
     2641 ccagcaccac caaggcgatc agcacgccga ccagcggcag cacgtaggcg cgcaacacca
     2701 gcccgccccg gccatggcgc accagcagcg ggatgtcgtc atagatgacg ttgcgctcca
     2761 ggcgcccgcg caacagccgc ccgccgaagt tgtaatagcg ggccagcgcg cgattggtcg
     2821 cggcgcgggg cagcagggtc gagcgcagca gcgtgtcgag cgccgtgtcg cgcagcaggc
     2881 ggcccagcgg ggccggacgg atgcgttcca tgccctcgcg caacacgcgc agcaaggcgg
     2941 cctccgccgc cgggtcgcgg aaacggccca gtagttgcac cgcctccacg cggtccaggt
     3001 cgcgcacgcc gtcgccgggc gaccagatcg tgcgctccag cccggcgatg atctcccgcc
     3061 gcaggcccgc ctcgtccgcg ttcatgggcg cgcccccgag acggcgcgcg gcctgccgag
     3121 cgcccgtccc agcgcgccca gggcgctcag cacgagcagc accagcacgg gggccagcag
     3181 caggtagagc aggtagggcg tgatgccgag cagcaccggc cccaccccgg cgctctgccc
     3241 ctggatcgtc cgcgtgaacg agtggctgct gaaggcgccg aacacgaaga agacatagat
     3301 caggccgccc gcccagcgca aaatcgcagc ccagaaggtg ccgccgcgga cggccagccc
     3361 gccctccagg gtgtgggcgc cggtttccag gtcgtgggcc agcatgtagc agggcagcgc
     3421 cagcagcggc agcgccagca aggcgacggc cagcgggcgg ctgcccgcgt cgggggcccc
     3481 gctcatggtg atgtccacca gcagcgccag gatgtagagc gacagcagca ccaccgcgcc
     3541 ggccaggatg ccgccgagca gcgccttgct gcgccgggtg ggcaactggg gcaacggcaa
     3601 ccgcggcccg aaccaggcca ccagcagcac caggccgatt tgatgcaggc tgaacacggt
     3661 gaggcccagg cccacgaaca gcaccggccc gataatcagg tcggcccatt gggtttgccc
     3721 ggagcgggtg cccgcccaga acagcacgcc caggacgatg accaccagca gcgcgatcag
     3781 cggcagcaca tagccgcgca gcaacacccc gacgcgcccg tgctgcacca gcagcgggat
     3841 gtcgtcgtag atcacgttgc ggtccaaccg cccgcggcgc aagcgccgcc acaggtcata
     3901 gtagaccgcc aactccttat cgaccacggc gcggggctgg gcggacgagc gcagcagcgt
     3961 gtcgagcgcc gtgtcgcgca gcaggcggcc cagtggcggc ccgatgcccc gcggcgcctc
     4021 gttcaagaca cgcagcaggg cgaccccggc ggcggggtcg cggaactggc cgagcagccg
     4081 cacggcttcg acccggtcga actcgctcac gccgggcggc ggcgtccaga tcgtgcgcgc
     4141 cagcccggcc aggatctcct ggcgcaacgc ggcggcgggc ggatcgggtt gctccaccgg
     4201 gtcagcctcc tcccgcgctg agcagcgcct tggccgccac aatggccgcg cggggatcgt
     4261 tttgctgcaa gatcccggca atcgccgcgt cgggctgggg cgccatggcc gcggccaggc
     4321 tcgtcacatc gtcggtggcc ggggtcagcg gacccagcag cacgccgggt tgctgggcgg
     4381 ccagggcgtt gaaatagtct tggtgcgtga gatgccagaa gcgcaccgtg tcgcgggtgg
     4441 cgggcgtgtc caggatcgtc gccgcgcaac tgaaatcgag caggtggtcg cgcgggtcgc
     4501 cctgcaaccc ttcgggcggc ggcaggccct ggaacaacgc cagcgggtcg ctcgccaggt
     4561 gcgctccggc ccaatcatgg ggcaccgacg tgccgcgata gccggcctgg cccatggccg
     4621 ccgccagttg cgccagcgcg gcgataatca ccgggcgcgg gaagaagagc gcgttttcct
     4681 ggtccttgag cgtggcccag ttcagcagca tctccacgaa ctgcttgaac agccccgact
     4741 ggctggtgag aaactccggc gccgccgcat tgccggccgg cagcgggaaa ttggcgacga
     4801 gttcggtgag catgtagagc aggaagggca cctggcccag gctgagcagc agcgggtcgc
     4861 ccgcgcgcag gcggtccagc agcccggcgg cctgccgctt ttgcagatag tcggcgatct
     4921 ggtcgacgct ccaggggtcg agcagcacgg tgtggaagtc gagcccgtcg aactcggtgg
     4981 tatagtcgag catccggcag gtgacgatgg tgcggatctg cgtgtactgg cggaaaaacg
     5041 cctggatctg ggccagccgg gcggcggcgg cgggcagcgg cagcgcgggc agttcgttga
     5101 ggccgtcgag catcagcagg atgcggccgt tctgcagata ggtctccagc gagtcggcca
     5161 tccaggcgct ttgcacatgg acgggcggct cgggcggctg cggattgggc gggttccgca
     5221 agaagttgcg cacgaagtcg agcatcggcg cggtcgtggt gtacatgctc aggtccacga
     5281 ccacgggcag caggccgggg atgggcgtgg tttcgggccg cgtgcattgc agcgccatat
     5341 attgcagata gctggtcttg ccgccgcccg gcttgccgag cagcaacacg cgccagaagt
     5401 cgcggatggc gtcgggcagg gcgatgggcc ggcgctgcaa ctggcgggcc gccgccgggg
     5461 cgccggggcg cgcgctcacc tgctgctgga acagcgccgc cgtgtcggtg gggatgaagg
     5521 ggctgccctg cgccgacgcg gggccgagga tatactggtt gcgccagtag gtgtagacgc
     5581 tggcgacctg gaacaggtac tgctgctcgt cggcggtgcg gtcctggccg atgacctggt
     5641 tgcccgcgga gagcggcgcg ttgatcacgg tggggccgaa gaactcgtag gtcgcgccgg
     5701 tgcccccgct gggcggcatc acgaactggg gccgggtcat agccactgcc ggcgccggtt
     5761 ggggattgtc gctcatcgcc gctcctcgcg caccgcgtgc atctgagtct tatgtcgact
     5821 agcctttccc agagtatagc atcaacccga tcccggcgca agcggcagag cccgcatccg
     5881 ttcccgcacg acgtgggttc accgcgacgg aacgaaggca acgaagatct acgcatgatt
     5941 tcaccgcgac ggaacgaagg caacgaagac gattaatata aaaaatcttc gttatcttcg
     6001 ttccttcgcg gtgaagccgg tcgcgggcaa atggtaaccc ggcccgccgt cgtcgcgata
     6061 ctatgtgtag taaactacga tctagctgcc gtccggatag ttcatcagca gtcagcgacc
     6121 ggcgcccgcc ggcccggtta gaggagagga ccagccatga gtcaggcgat gtatcggatt
     6181 cgtactgcgc tgctgcttgc cgccgtcctc gcgcccgccc tgggtggggc cggcgcccgc
     6241 cccccggcgg ccctggccga cccggtgacg cccagcgcgc ccgtgtttgc cgattcggcg
     6301 ttcagccgcg tctg
//
LOCUS       DASUYC010000652         3515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1197023, whole genome shotgun sequence.
ACCESSION   DASUYC010000652 DASUYC010000000
VERSION     DASUYC010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197023"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VF276_10600"
     CDS             <1..494
                     /locus_tag="VF276_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607350.1"
                     /translation="AREPGSDPLAGYSAGLFGITGKAVVELLLARGPLTMQQIRLGLG
                     QHKRFLIHDTPLVMAELERALTVIAGGPELAAAWRPGAGAARAPARPRSRFNPPPSKD
                     LDLRVWELTMRWAPPAALAAADRLREHPPQAREGLCARLRALHPGVTGAELDALLGED
                     PHP"
     gene            491..3409
                     /locus_tag="VF276_10605"
     CDS             491..3409
                     /locus_tag="VF276_10605"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEX6607351.1"
                     /translation="MSRRPPNPPDPTPPPGGQRRPASLRDEYEERFVRGRGLPLKGQP
                     PRRPAPPVASRLPGGCLILVAVVVLLFILGPILLEVSVDWQWFGSLGLQSVYGTRLTA
                     AIGVFAAGLVVAALFFAINWLIARRIAVPRDLFPGQQLDVSQRLLGLGIGVAVVIVSL
                     FMGFIAAGEWTTALNYFNHTAFGATDPIFHQDIGFYVFELPFYDFLRGWATALVVLAA
                     IGVGAIYATRPTLFTAGGRFTPDPHVVAHISVLAVAFLLLTGVGYWFRSYDLLFSTHD
                     ALYGASYTDVNARLLAFRILLVVTIAIALLLLVNLRVRALIPLAAAVGVWLLAQFVVG
                     GLYPNAVQSFSVKPAELDKERPYILNNIAATRAAFGLDTFRESPTDASTRLTRADVES
                     NRNLVDSIRLWDYRPLLQTYTQLQALRPYFVFHGVDLDRYPLAGGEAQVMISAREINT
                     DGLAETARTWLNQHLVYTHGYGVVASPVNQIESGGQPHFIVRDFPVVSEDPALKVTVP
                     QIYYGEAEENYAVVNTKAEEFDYPAGDHDVMTKYQGTGGIAIGNFLNRLQLAAYFGDF
                     TLLISDLIEDDSRVLFHRQIGDMVQRVAPFLSYDADPYIVIADGKLYWIQDAYTTSDR
                     YPNSTPLDTDYGTVNYIRTSVKVVVDAYNGSMSFYVVDPKDPVIATYRRIFPGLFKDA
                     GEMPASLQAHVRYPEGLFNIQARTYDTFHMTDPQVFYNKGDAWRVPFGNQTDTSAPLE
                     AYYTMMRLPGETTNQFMLMVPFTPASKQNMIAWMAGRGDAPDYGRVDVIRFPASTTVF
                     GPQQVDATISQDTQVSSQISLWNQGGSQVVRGNMLVIPIASSVLYVQPLFLQATSSPL
                     PELKRVIAAAGGQVGMGEDLNSALDAMFGSRAAPPPVGPGTGGTPTAGNTPFPTFPAG
                     GPSPTAGACVGDAASLSADALDHYQRAQDALKVQDWATYGREQALVEADLRCLQQVTR
                     "
BASE COUNT          523 a         1293 c         1119 g          580 t
ORIGIN      
        1 ttgcccgcga gccgggcagc gatcccctgg ccgggtacag cgcgggcctg ttcggcatca
       61 ccggcaaagc ggtggttgaa ctgctgctgg cccgtggccc gctcaccatg cagcagatcc
      121 gcctggggct gggccagcac aagcgcttcc tgatccacga cacgcccctg gtgatggccg
      181 aactggagcg cgccctgacc gtgatcgccg gggggccgga actggccgcc gcctggcgcc
      241 ccggtgcggg cgcggcccgc gcgccggccc gcccgcgctc gcgctttaat ccgccgccca
      301 gcaaagatct cgatctacgc gtctgggaac taaccatgcg ctgggcgccg cccgccgccc
      361 tggccgccgc cgaccgtttg cgcgagcatc cgccgcaggc ccgcgagggc ttgtgcgccc
      421 ggctgcgcgc cctccatccc ggcgtcaccg gcgccgagtt ggacgcttta ctaggagagg
      481 acccgcaccc atgagccggc gaccccccaa cccccctgat cccacgccac cccccggcgg
      541 ccagcgccgg cccgccagct tgcgcgacga atacgaagag cgctttgtgc gcgggcgcgg
      601 cctgccccta aaggggcagc cgccccgccg gcccgccccg ccggtagcct cgcgcctgcc
      661 cggcggctgc ctgatcctgg ttgccgtcgt cgtgctgctg ttcatcctgg ggccgatcct
      721 gctggaggtg tccgtcgatt ggcagtggtt cggcagcctg ggcttgcaaa gcgtctacgg
      781 cacgcggctg accgcggcca tcggcgtctt cgccgccggc ctggtggtgg ccgcgctgtt
      841 cttcgcgatc aactggttga tcgcccggcg catcgccgtg ccgcgcgacc tgttccccgg
      901 ccagcaactg gatgtctcgc agcggctgct gggcctgggc atcggcgtgg ccgtcgtaat
      961 cgtcagcctg ttcatgggct tcattgccgc gggcgagtgg accacggcgc tgaattactt
     1021 caaccacacg gctttcggcg caaccgaccc gatcttccac caggatatcg gcttctacgt
     1081 ctttgagttg cccttctacg acttcctgcg cggctgggcc acggcgctgg tggtgctggc
     1141 cgccatcggc gtgggtgcca tctacgccac ccggcccacg ctgttcaccg cggggggccg
     1201 cttcacgccc gacccgcacg tggtcgccca tatcagcgtc ctggccgtgg ccttcctgct
     1261 gctgaccggc gtgggctact ggttccgcag ctacgatctg ctgttctcca cgcacgacgc
     1321 cctctacggc gcctcctaca ccgacgtgaa cgcccggctg ctggccttcc gcatcctgct
     1381 cgtcgtgact atcgccattg ccttgctgct gctggtcaac ctgcgcgtgc gcgcgctgat
     1441 cccgctggcc gccgcggtcg gcgtctggct gctggcgcag tttgtggtcg gcggcctcta
     1501 tcccaacgcc gtgcagtcgt tcagcgtgaa gcccgccgaa ctcgacaaag agcgccccta
     1561 tatcctcaac aacatcgcgg ccacccgcgc ggcgttcggc ctcgatacct tccgcgaaag
     1621 cccgaccgat gccagcacgc gcctgacccg cgccgacgtg gagtcgaacc gcaacctggt
     1681 tgacagcatc cgcctctggg actaccgccc gctgttgcag acctacaccc agttgcaggc
     1741 gttgcgcccc tacttcgtct tccacggggt ggacctggac cgctatccgc tggccggggg
     1801 cgaagcccag gtgatgatct cggctcgcga gatcaacacc gacggcctgg ccgagacggc
     1861 gcgcacctgg ctgaaccagc acctggtcta cacccacggc tacggcgtgg tcgccagccc
     1921 ggtgaaccag atcgagagcg gcggccagcc gcactttatc gtccgcgact tcccggtggt
     1981 cagcgaggac ccggccctga aggtgacggt gccgcagatc tactacggcg aagccgagga
     2041 gaactacgcc gtcgtcaaca ccaaggccga ggagttcgat tacccggcgg gcgaccacga
     2101 cgtgatgacc aagtaccagg gcacgggcgg gatcgccatc ggcaacttcc tcaaccggct
     2161 ccagcttgcc gcctatttcg gcgatttcac tctgctgatc tccgacctga tcgaggacga
     2221 cagccgggtg ctgttccatc gccagatcgg cgacatggtg cagcgggtgg cgcccttcct
     2281 gagctacgac gccgacccct atatcgtcat cgcagacggc aagctctact ggatccagga
     2341 cgcctatacc accagcgacc gctatcccaa ctcgacgccg ctggacaccg actacggcac
     2401 ggtgaactat atccgcacct cggtgaaggt ggtcgtcgac gcgtataatg gctcgatgag
     2461 cttctacgtc gtcgatccca aggacccggt gatcgccacc taccggcgca tcttccccgg
     2521 cctgttcaag gatgccggcg agatgcccgc atccctgcag gcgcacgtcc gatacccgga
     2581 gggcctgttc aacatccagg cgcgcaccta cgacacgttc cacatgaccg acccgcaggt
     2641 gttctacaac aagggcgacg cctggcgcgt gcccttcggc aaccagaccg atacgagcgc
     2701 gccgctggaa gcctactaca cgatgatgcg cctgccgggc gagaccacca accagttcat
     2761 gctgatggtg ccgttcacgc ccgcgtcgaa gcagaacatg atcgcctgga tggccggacg
     2821 cggcgatgcg ccggactatg gccgcgtgga tgtgatccgc ttcccggcga gcaccaccgt
     2881 gttcgggccg cagcaggtcg acgccacgat cagccaggac acgcaggtct ccagccagat
     2941 ctcgctctgg aaccagggcg gcagccaggt ggtgcgcggc aacatgctgg tcatcccgat
     3001 tgccagctcg gtgctgtatg tccagccgct gttcttgcag gccaccagca gcccgctgcc
     3061 cgaacttaag cgggtgatcg cggcggcggg cggtcaggtg ggcatgggcg aggacctgaa
     3121 ctcggcgctc gatgccatgt tcgggtcgcg cgcggccccg ccgccggtcg ggccgggcac
     3181 cggtggcacc cccacggcgg gcaacacgcc cttcccgacc ttcccggcgg gcgggccgtc
     3241 gcccaccgcg ggggcctgcg tgggtgacgc cgccagcctg agcgccgacg cgctcgacca
     3301 ctaccagcgc gcgcaagacg cgctcaaggt ccaggactgg gcgacctatg gccgcgaaca
     3361 ggccctggtc gaagccgatc tgcgctgctt gcagcaggtc acgcggtagc gcctttggtc
     3421 gcgcgcgttc gcgccttcgc gcaggcccac gcggagccgc tagccgtctg gctggcgctc
     3481 cgcgtgggcc tcacgctgct ggcgattctg gccgg
//
LOCUS       DASUYC010000653         4935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_485388,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000653 DASUYC010000000
VERSION     DASUYC010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485388"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /gene="tuf"
                     /locus_tag="VF276_10610"
     CDS             <1..981
                     /gene="tuf"
                     /locus_tag="VF276_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX6607352.1"
                     /translation="KRHYAHVDCPGHADYIKNMITGAAQMDGAILVVSAPDGPMPQTR
                     EHILLARQVQVPAIVIFLNKVDMMDDEELLELVELEVRELLSKYNFPGDDVPIIRGSA
                     LAALTSTSKDPNAPEYAPIKELMAAVDDYIPTPERAVDRPFLMPIEDVFGIKGRGTVT
                     TGRIERGQVKVGDTIEIVGMRETRTTVVTGVEMFQKTLDSGIAGDNVGTLLRGVERTD
                     VERGQVLAKPGSIKPHTKFFAEVYVLSKEEGGRHTPFFSGYRPQFYIRTTDVTGNITL
                     QEGVEMVMPGDNTQMTVELIVPVAIEEGLRFAIREGGRTVGAGVCTKIIE"
     gene            1072..1380
                     /gene="rpsJ"
                     /locus_tag="VF276_10615"
     CDS             1072..1380
                     /gene="rpsJ"
                     /locus_tag="VF276_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEX6607353.1"
                     /translation="MAKQKIRIRLKAYDHKILDQSAQQIVETAERTGALVAGPVPLPT
                     KIEKHSVIRGPFIDKDSQEQFEIRTHKRLIDVLDPNQKTISALMRLNLPAGVDIEIKL
                     "
     gene            1483..2136
                     /gene="rplC"
                     /locus_tag="VF276_10620"
     CDS             1483..2136
                     /gene="rplC"
                     /locus_tag="VF276_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEX6607354.1"
                     /translation="MIKGILGRKVGMTQIFEATGTAAGVTVVQAGPCTVTQVKTPEKD
                     GYQAVQIGFEELTGRQERKLTDPQKGHLRKNKLPLLRHLREVEVGGDDAASHNVGDKI
                     DVTMFAVGDLVDVIGTSKGKGFAGVVKRHHFRGGPKTHGQSDRHRRPGSIGAGTTPGR
                     VYKGMRMAGQMGNERVTVQNLRVLQIDTERNLLVLRGAVPGPAGSLITVRKAIKGTR"
     gene            2262..3143
                     /gene="rplD"
                     /locus_tag="VF276_10625"
     CDS             2262..3143
                     /gene="rplD"
                     /locus_tag="VF276_10625"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEX6607355.1"
                     /translation="MQATIYNLQGQALDTVELNDAIYGIEPNIGVVHQAVLRQQANAR
                     LGTHNTLTRSDVRGGGKKPWRQKGTGRARQGSTRAPQWRHGGVVFGPHPRSYAQDLPR
                     KMRRLAMRSVLSAKAADGQILVIDNFADLEPRTKVMLSTLAALNLADRRVLIMTPERH
                     DNLELAAGNLKNVTTMRTQYLSLVAMLKAEILVLDRASLTVIESILGQTGGRTSRPIA
                     APEGAARSRLTSVLDGEPAAPAAAAPAAGGATVTSTITAAPPPATSPVESPVETEAET
                     AAADTEALETQGEEENA"
     gene            3143..3448
                     /gene="rplW"
                     /locus_tag="VF276_10630"
     CDS             3143..3448
                     /gene="rplW"
                     /locus_tag="VF276_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEX6607356.1"
                     /translation="MDSKLYDVIRRPVMTEKNSLLMEQGKYTFEVSRDATKAMIKRAV
                     EQAFPNVKVQAVNIVNVHAKRRNFSHKRKRISDWGPRWKKAVVTLNPGQHIEIFEGV"
     gene            3452..4276
                     /gene="rplB"
                     /locus_tag="VF276_10635"
     CDS             3452..4276
                     /gene="rplB"
                     /locus_tag="VF276_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEX6607357.1"
                     /translation="MALKRYKPTSPGRRLTTVATFEEITAKKPEKSLIEIKKSNAGRN
                     NQGRITVRHRGGGHKRFYRIIDFKRNKLGVPGRVATIEYDPNRSARIALIHYADGEKR
                     YILAPLGLKVGDTIMSGSGAEVRVGNTLPIHSIPLGTQIHNIELQAGRGGQLVRSAGE
                     SAQLMARDETYAQVRMPSGEVRRIHVNCMATIGQVGNLDHENIRLGKAGRSRWLGYRP
                     TVRGSVMNPRDHPHGGGEGRTPRGGQPQTKWGKPAHGRTRNNPRTDNMIVRKRYAK"
     gene            4294..4578
                     /gene="rpsS"
                     /locus_tag="VF276_10640"
     CDS             4294..4578
                     /gene="rpsS"
                     /locus_tag="VF276_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEX6607358.1"
                     /translation="MSRSTKKGPFVEERLLNRIKQMNSTGAKRVIKTWSRDSTVFPEM
                     VGHTIGVHDGRRHVPIYITENMVGHKLGEFALTRFFKGHIKGAERMHRAK"
     gene            4597..>4935
                     /gene="rplV"
                     /locus_tag="VF276_10645"
     CDS             4597..>4935
                     /gene="rplV"
                     /locus_tag="VF276_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEX6607359.1"
                     /translation="MEVRATAKFIRSSPRKVRLVLDVVRGKPVKEALAILRFMPQHAA
                     REVMSVVASAAANAENNFQMAPDDLVITSIYANEGPTFKRFRAKARGQGTRILKRTSH
                     ITVVVDEREGI"
BASE COUNT          986 a         1559 c         1669 g          721 t
ORIGIN      
        1 aagcgccact acgcccatgt ggactgcccc ggccacgccg actacatcaa gaacatgatc
       61 accggcgccg cccagatgga tggcgccatc ctggtcgtgt cggcccccga cggccccatg
      121 ccccagaccc gcgagcacat cctgctggcg cgccaggtcc aggtgccggc catcgtcatc
      181 ttcctcaaca aggtcgatat gatggacgac gaggaactgc tggagttggt cgaacttgaa
      241 gtgcgcgaac tgctcagcaa gtacaacttc cccggcgacg acgtgccgat cattcgcggc
      301 tcggcgctgg cggcgctgac gagcacgagc aaggacccca acgcgccgga gtacgcgccg
      361 atcaaggagt tgatggcggc ggtggacgac tacattccga cgccggagcg ggcggtggac
      421 cggccgttcc tgatgccgat cgaggacgtg ttcgggatca agggccgggg cacggtgacg
      481 acggggcgca tcgagcgcgg gcaggtgaag gtcggggaca cgatcgagat cgtgggcatg
      541 cgcgagacgc ggacgacggt ggtgacgggc gtggagatgt tccagaagac gttggactcg
      601 gggatcgcgg gggacaacgt ggggacgctg ctgcgcgggg tggagcggac ggacgtggag
      661 cgggggcagg tgctggcgaa accgggcagc atcaagccgc acacgaagtt cttcgccgag
      721 gtgtacgtgt tgagcaagga agaggggggg cggcacacgc cgttcttctc ggggtaccgg
      781 ccacagttct acatccggac gacggacgtg acgggcaaca tcacgctgca ggaaggggtg
      841 gagatggtga tgccggggga caacacgcag atgacggtgg agttgatcgt gccggtggcc
      901 atcgaggaag ggttgcggtt cgcgatccgg gaaggcgggc gcacggtggg agccggcgtc
      961 tgcaccaaga ttatcgagtg acacagccag gcggctggac gggctggggc tacccccgca
     1021 ggggtagcgc cgcccgctcg ccggtgatcg gcgaaaggga gaattaggag cgtggcgaag
     1081 caaaagattc gcatccgcct gaaggcgtac gaccataaaa ttctggacca gtccgcgcag
     1141 cagatcgtcg agacggctga gcggacgggg gctctcgtag ccggcccggt tccgctgccg
     1201 acgaagatcg aaaagcacag cgtgatccgc ggccccttca tcgacaaaga ctcgcaagag
     1261 cagttcgaga tccgcactca taagcggttg atcgacgtgc tggatccgaa ccagaagacg
     1321 atcagcgcgc tgatgaggct caacctgccg gccggcgtcg atatcgagat caagctgtaa
     1381 ggcagcgccg gcacctgtgg ccgatagccc ggctgtttgc cggggtctct cggcccgcca
     1441 gtacacgttc agactatagc tgaacagagg gttttgagca ccatgattaa gggtattctg
     1501 ggccgcaaag tcggcatgac ccaaattttc gaggccaccg ggaccgccgc gggcgtgacc
     1561 gtggtgcagg ccggcccctg caccgtcact caggtgaaaa caccggagaa agacggctat
     1621 caggcggtgc agatcggctt cgaggagctg accggccgcc aggagcgcaa gctcaccgac
     1681 ccgcagaagg gccacctgcg caagaacaag ctgccgctgc tgcgtcacct gcgcgaagtc
     1741 gaagtcggcg gcgacgatgc ggccagccac aacgtgggcg acaagatcga tgtgaccatg
     1801 ttcgccgtgg gcgacctggt tgatgtcatc ggcacgtcca agggcaaggg ctttgccggt
     1861 gtggtcaagc gccaccactt ccgcggtggt cccaagacgc acggccagtc cgaccgccac
     1921 cggcggcccg gttccatcgg cgcgggcacc acgccgggcc gggtttacaa gggcatgcgc
     1981 atggccgggc agatggggaa cgagcgcgtg accgtgcaga acctgcgcgt tctgcagatc
     2041 gacaccgagc gcaacctgct ggttctgcgc ggcgcggttc cggggcctgc gggcagcctg
     2101 atcaccgtcc gcaaggccat caaggggacg cggtagaccg ccaagcaggt ccgcccctgc
     2161 gaggccccac ggtccctcag ggtcacggcc ggcacgtcgg agcctcaggc ttgtttgtgc
     2221 cccggtatcc gaccgctgct ttatcgtaat ggagaaaaag agtgcaggca accatctata
     2281 atttacaggg ccaggccctc gacacggtgg agctgaacga tgcgatctat ggcatcgagc
     2341 ccaacatcgg tgtcgtgcac caggccgtgc tgcgccagca ggccaacgcc cggctcggca
     2401 cgcacaacac gctgacgcgg tcggacgtgc gcggcggcgg caagaagccg tggcgccaga
     2461 agggcaccgg ccgggcgcgc cagggctcca cccgtgcccc ccagtggcgg catggcggcg
     2521 tcgtgttcgg cccccatccg cgctcctatg cccaggatct gccgcgcaag atgcgccggc
     2581 tggcgatgcg ctccgtcctc tcggccaagg ccgcggatgg gcagatcctc gtgatcgaca
     2641 actttgccga cctggagccg cgcaccaagg tcatgctgag caccctggcc gcccttaacc
     2701 tggccgaccg gcgcgtgctg attatgacgc cggagcgcca tgacaatctg gaactcgccg
     2761 ccggcaacct gaagaacgtc accaccatgc gcacgcagta tttgagcctg gtggcgatgc
     2821 tgaaggcgga aatcctggtc ctcgaccggg ccagcctcac cgtcatcgag agcatcctgg
     2881 gccagaccgg cggacgcacg tcccgcccga tcgccgcccc ggaaggcgcg gctcggtccc
     2941 gcctgacgtc ggtgctggac ggcgagcctg cggcccccgc ggcggcagcg cccgcggccg
     3001 gcggcgcgac ggtgacctcg accatcacgg cggctccgcc ccccgccacg agcccggtgg
     3061 aatccccggt ggagaccgag gccgaaaccg cggctgcgga caccgaagcc ctggagacgc
     3121 agggtgagga ggagaacgcc taatggacag caagctttac gacgtcatcc gccgtccggt
     3181 catgaccgag aagaacagcc tgctcatgga gcaggggaag tacaccttcg aggtctcccg
     3241 cgacgcgacc aaggcgatga tcaagcgtgc cgtggagcag gccttcccca atgtcaaggt
     3301 gcaggccgtc aatatcgtca acgtgcatgc caagcgccgc aacttcagcc acaagcgcaa
     3361 gcggatctcc gattggggcc cgcgctggaa gaaggcggtt gtcacgttga accccggcca
     3421 gcatatcgag atttttgagg gagtctaagc catggcactg aaacggtata agccgacttc
     3481 gccgggccgc cgcttgacca ccgtggctac cttcgaggag atcacggcca agaagcccga
     3541 gaagtcgctc atcgagatta agaagagcaa cgccgggcgg aacaaccagg gccgcatcac
     3601 cgtgcgccat cgcggcggcg gccacaagcg gttctatcgg atcatcgatt tcaagcgcaa
     3661 caagctgggc gtgcccggcc gcgtggcgac catcgagtat gatcccaacc gctcggcgcg
     3721 catcgcgctc atccactatg cggatggcga gaagcgctat atcctggcgc cgctcggcct
     3781 caaggtcggc gacaccatca tgtcgggcag cggcgcggaa gtgcgcgtcg gcaacacgtt
     3841 gccgatccac agcatcccgc tcggcaccca gatccataac atcgaattgc aggcgggccg
     3901 cggcggccag ctcgtgcgca gcgcgggcga gtcggcccag ttgatggcgc gcgacgaaac
     3961 ctatgcccag gtgcgtatgc cgtcgggcga ggtgcgccgc atccatgtga actgcatggc
     4021 gaccatcggc caggtgggca acctcgacca cgagaacatc cgcctgggca aggccgggcg
     4081 cagccgctgg ctgggctatc ggcccacggt gcgcgggtcc gtgatgaacc cgcgggacca
     4141 tccgcatgga ggtggcgaag gccgcacgcc gcgtggcggg cagccgcaga ccaagtgggg
     4201 caagccggcc catggccgca cccgcaacaa cccgcggacc gataacatga tcgtgcgcaa
     4261 gcgttacgcg aaataggctg acccggagga accatgagtc gctctacgaa aaaggggccg
     4321 tttgtcgaag aacggctgct gaaccgcatc aagcaaatga acagcacggg cgccaagcgc
     4381 gtgatcaaga cctggtcgcg ggactccacc gtcttcccgg agatggtggg gcacaccatt
     4441 ggtgtccacg acggccgtcg ccatgtgccg atttatatca ccgagaacat ggtgggccac
     4501 aagcttggcg aattcgctct tacgcgcttc ttcaagggcc acatcaaggg cgccgagcgc
     4561 atgcatcgcg ccaagtaaga ggagagggtt acaccaatgg aagtacgcgc taccgcgaag
     4621 tttatacgca gttcgccgcg caaagtccgc ctggtgctcg acgtggtccg cggcaagccg
     4681 gtgaaggaag cgctggcgat cctgcgcttc atgccgcagc acgccgcccg cgaggtgatg
     4741 agcgtggtgg cttcggccgc cgccaacgcg gagaacaatt tccagatggc tccggatgac
     4801 ctggtcatca ccagcatcta cgccaatgaa gggcctacct tcaagcggtt ccgggccaag
     4861 gcacgcggcc agggtacccg tatcctgaag cgtaccagcc acattaccgt tgtggtcgac
     4921 gaacgggagg gtatc
//
LOCUS       DASUYC010000654         3913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_622002,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000654 DASUYC010000000
VERSION     DASUYC010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622002"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            233..1354
                     /locus_tag="VF276_10650"
     CDS             233..1354
                     /locus_tag="VF276_10650"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX6607360.1"
                     /translation="MVLTQSCGRTAVGALLAAVWHQPEHTVRQRLREWTYEAAAKKGR
                     KRCEVEVRTCFAPLLRWVLTYWPASDAHLALVLDATSLRDRHTVLACSVVYRGCAIPV
                     AWTVVAGNRKGAWKPHWLAMLEDLDGVLPAGWTVLVLADRALYARWLYQRIVANGWHP
                     FLRIQRQGFFRRAGQGHWHDLAHLLPQAGTTWSGAVTCFKNQEGQLECTLLARWEEGY
                     RDPWLIVTDLPVEVAEAAWYGLRTWIEAGFKDLKRGGWHWEQTKLREPERIARHWLVL
                     AVATLWVTSVGGEAEATGPVSGLERLPVLHIARRTCKGRSQARLLSCFRRGVVQILAG
                     VVSGVGVAMGNFWPEAWPMREGAEPLKLVDVAAREALAA"
     gene            1495..3015
                     /locus_tag="VF276_10655"
     CDS             1495..3015
                     /locus_tag="VF276_10655"
                     /inference="COORDINATES: protein motif:HMM:NF016988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEX6607361.1"
                     /translation="MQPTPLDGPPDAGSAAWDATGEPPAGSAHSQGRRARYAEYAAFY
                     RGDQWPDLPAPGERRLTFNYARVFVNKAASYLLGKPVGYDATALAPGEPGAGGRAATT
                     PRRGRRAGGEARAVEAYLEAVAAFNHLAALDLDTAISTGTLGDGAWTVRWDAAAGLPR
                     VTAVDPTGLDARWRADDLTTLLWVRQQYWTSRAELTAAQRARLPAGTPADPYHPLRAR
                     ETWTPRHWRLEVGDAVLDGGPNPYGQIPYVIFPNLRVPGEFWGESDLVDLLRLQRELN
                     ARLSIFSRILEVAGNPVAVVIGADADSTDSLKLGPNQLWTLPEGASADVLDLLKAGGA
                     EAHLRYIDTIYRAMHDISEMPRTSFGDSGGASAARSGVALEIELQPLLHKLARKRAIW
                     STALQRRAHLILRVARLHGADLPPAVLALTWPPVLPQDRAALVAQETALVHAGIHPPT
                     RAMALLNDPDPDALLGQVLDQAARLHGAGVRLGGQSAPKATSAHDPSTPAVAGDSP"
     gene            3012..3743
                     /locus_tag="VF276_10660"
     CDS             3012..3743
                     /locus_tag="VF276_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607362.1"
                     /translation="MSITRTALRTMLRAALLDTNAPLLWTDTALDGAIDEAVTAHSYQ
                     FPCPVAARYSLGAGQQVVYIVPTDPALPEDVPVPSGTINCDVIGVQRAELPLGTPLPL
                     DPGQSTDPAASGSARYRQGYRWRGHTLVLRNPASGDEVGAYTLRVEYLQTYNIPLDTP
                     LVVPWNGPDSDIPLLLLLAKRAAYQLLAEWQVRTQGATPPTLASGSSLAHIAVPPILA
                     ALEVEIARALAFRRLRSLRSRALDI"
BASE COUNT          582 a         1472 c         1320 g          539 t
ORIGIN      
        1 ttcccgcggc gggctttcaa gggtaggtat ctggcgaggc ggggggccgg ggccggaaag
       61 gacggacgag accgggatct tttggtatga tagtgctctc atacactcta tcaccccagg
      121 aggatcccat gtctcgtccg gaatcattat cgctgtggac tgccaccgtg tcaatgcatc
      181 tgccccatct ctcgcagccc aactcgtggt gctcgccgcc tggagttggg ccatggtcct
      241 cacccaatcg tgcgggcgca ccgctgtcgg tgccctgctc gccgccgtct ggcaccaacc
      301 cgaacacacc gtccgccaac ggctgcgcga gtggacctat gaagcggccg ccaaaaaggg
      361 ccgcaaacgc tgcgaggtcg aggtccgcac ctgctttgcg ccgctgctgc gctgggtgct
      421 gacctattgg ccggccagcg acgcccatct ggcgctcgtg ctggatgcca ccagcttgcg
      481 cgaccgccac accgtgctgg cgtgcagcgt cgtctaccgc ggctgcgcca tcccggtggc
      541 ctggacggtg gtcgcgggca atcgcaaagg cgcctggaaa ccccattggt tggcgatgtt
      601 agaggacttg gatggggtgc taccggcggg ctggaccgtg ttggtgctgg ccgaccgggc
      661 gctgtatgcg cgctggttgt accagcgtat cgtcgccaac gggtggcacc cgttcttgcg
      721 catccagcgg caaggcttct tccgccgcgc cgggcagggc cattggcacg acttggcgca
      781 cctgttgccg caggcgggca cgacctggag cggggcggtg acgtgtttca agaaccaaga
      841 gggccaactg gagtgcacgt tgctggcgcg ttgggaggag ggctaccgcg acccatggct
      901 gatcgtgacc gacttgccgg tggaggtcgc agaggcggcg tggtatgggc tgcggacctg
      961 gatcgaggcg gggttcaagg acctcaagcg cggcggctgg cattgggagc agaccaagct
     1021 gcgcgagccg gagcggatcg cgcggcactg gctcgtgctg gcggtggcga ccttatgggt
     1081 gacgagtgtg ggcggggagg cggaggcgac ggggccggtg agtgggttgg agcgcttgcc
     1141 ggtgctgcac attgcgcggc ggacgtgcaa agggcgcagt caagcgcgct tactgagttg
     1201 ttttcggcgc ggggtggtgc agatcctggc gggggtcgta agcggggtgg gggtggcgat
     1261 gggcaacttc tggccggagg cgtggccgat gcgggagggc gcggagccac tgaaactggt
     1321 ggacgtagcg gccagggagg ccctggccgc ctagatacct acccttgaaa gcccgccgcg
     1381 gggaggggtc aggggagagg ttgccgcccc accgaccacc cgcccatcac cgaccccccg
     1441 tccccgtctc ctgacggctg acggctgacg gctaatcgca cgaaaggagc ccccatgcaa
     1501 cccacccccc tagacggccc gcccgacgcc ggcagcgccg cctgggacgc caccggcgag
     1561 ccgcccgccg gcagcgccca cagccaaggc cgccgcgccc gctacgccga gtatgccgcg
     1621 ttctatcgcg gcgaccagtg gcccgacctg cccgcgcccg gcgagcgccg gctcaccttc
     1681 aactacgccc gcgtcttcgt caacaaggcc gcgtcctacc tgctgggcaa gccggtcggc
     1741 tacgacgcga ccgcgctggc gccgggcgaa ccgggcgcgg gcgggcgggc ggcgaccacg
     1801 ccgcgccggg gccggcgggc cggcggcgaa gcccgcgccg tggaagccta cctggaggcc
     1861 gtggccgcct tcaaccacct ggccgcgctc gacctcgaca ccgccatcag caccggcacc
     1921 ctgggcgacg gcgcctggac cgtgcgctgg gacgccgccg ccgggctgcc ccgcgtcacc
     1981 gccgtggacc ccaccggcct cgacgcccgc tggcgcgccg acgacctgac caccctgctc
     2041 tgggtgcgcc agcaatactg gaccagccgc gccgaactca cggcggccca gcgcgcccgg
     2101 ctccccgccg gcacgcccgc cgacccctac cacccgctgc gcgcccgcga gacctggacc
     2161 ccacgccatt ggcggctcga agtgggcgat gccgtcctcg acggcggccc caacccctac
     2221 gggcagatcc cctacgtgat cttcccgaac ctgcgcgtgc cgggcgagtt ctggggcgaa
     2281 agcgacctgg tggacctgct gcggctgcaa cgcgaactga acgcccggct gagcatcttt
     2341 tcgcgcatcc tcgaagtggc cggcaacccg gtggccgtgg tcatcggggc cgatgccgac
     2401 agcaccgaca gcctgaagct ggggccgaac cagctctgga ccttgccgga aggcgccagc
     2461 gccgacgtgc tcgacctgct caaagccggc ggcgcggagg cccacctgcg ctacatcgac
     2521 acgatctacc gcgccatgca cgacatcagc gagatgccgc gcaccagctt cggcgacagc
     2581 ggcggcgcct cggcggcccg ctcgggcgtt gcgctcgaaa tcgaactcca gcccctgctg
     2641 cacaaactgg cgcgcaagcg ggccatctgg agtaccgccc tgcaacgccg cgcccacctc
     2701 atcttgcgcg tggcccggct ccacggcgcc gacctgccgc ccgccgtgct cgcgctgacg
     2761 tggccgccgg tcttgccgca ggaccgcgcc gcgctggtgg cccaggagac ggccctggtc
     2821 cacgccggca tccacccgcc cacgcgggcc atggccctgc tcaacgaccc cgaccccgac
     2881 gccctgctcg gccaggtgct cgaccaggcc gcccggctgc acggcgccgg ggttcggctc
     2941 ggcggccaat ccgcccccaa agcgacctcg gcccatgacc catccacccc cgccgtggca
     3001 ggagacagcc catgagcatc acccgcaccg ccctgcgcac catgctgcgc gccgcgctgc
     3061 tggacaccaa cgccccgctg ctctggaccg acacggcgct ggacggggcc atcgacgaag
     3121 ccgtcaccgc ccacagctac cagttcccct gcccggtcgc cgcccgctac tcactcggcg
     3181 ccggccagca ggtcgtctac atcgtgccca ccgaccccgc cctgccggag gacgtgcccg
     3241 tgccgtcggg caccatcaac tgcgacgtga tcggcgtgca gcgggccgag ttgccgctgg
     3301 gcacgcccct gccgctcgac cccggccaga gcaccgaccc cgccgccagc ggcagcgcgc
     3361 gctaccgcca gggctaccgc tggcgcggcc acaccctcgt cctgcgcaac cccgcgagcg
     3421 gcgacgaagt gggcgcctac accctgcgcg tcgaatatct ccagacctac aacatcccgc
     3481 tggacacccc gctcgtcgtc ccgtggaacg gcccggactc ggacatcccg ctgctgctgc
     3541 tcctggccaa gcgcgccgcc taccagctcc tggccgaatg gcaggtccgc acgcaaggcg
     3601 ccaccccgcc cacgctggcg agcggcagca gcctcgccca catcgccgtc ccgcccatcc
     3661 tggccgcgct cgaagtcgaa atcgcccgcg ccctcgcctt ccgccgcctg cgcagcctcc
     3721 gcagccgcgc cctcgacatc tgacgcgcgg tcagccgtca gccgtcagct atcagctatc
     3781 aggagacgag tggtcagtgg tcagtggtca gtggtcagca ttggcaacgg atgccggtgt
     3841 aggggcgggg ttcaaaccgg cccgcgccgc cccaccggga tgcctgtcat tctgagcgca
     3901 gcgaagaatc tcg
//
LOCUS       DASUYC010000655         3456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1464559, whole genome shotgun sequence.
ACCESSION   DASUYC010000655 DASUYC010000000
VERSION     DASUYC010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1464559"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VF276_10665"
     CDS             complement(<1..285)
                     /locus_tag="VF276_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEX6607363.1"
                     /translation="MTARREFSPSRLAGEPPWLAALGLRLDDAHDGLLQCGIDVIEIP
                     RIAAAIERWGQPLLDRVWTPGEQRFCRGRTPELAARFAAKEAISKTLGTGI"
     gene            complement(282..1580)
                     /locus_tag="VF276_10670"
     CDS             complement(282..1580)
                     /locus_tag="VF276_10670"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEX6607364.1"
                     /translation="MSKISVVGVNYVGLVTAACFADLGNEVMGIEIVPEKVERLRQGI
                     SPIYEPGIEEVLQRNLKAGRLNFTTDYAEGLRGADFVFIAVGTPEGPDGAADMSQVES
                     AARSIGKHASNDLIVINKSTVPIGTGDLVTRLVGEHIKPGLKFSVVSNPEFLREGAAL
                     GDFMVPDRVVLGSSDREASEKVAGLYEPLNAPIIISDIRTAEMIKYASNAILATRISF
                     INEIAHICETLGADVKQVALGMGYDKRIGHQFLDAGIGYGGSCFPKDVKALAHMAASN
                     DCHPQLLNAVMDINTDARRLFVEKVTNLLGGDLHGKRIGVWGVSFKPNTDDIREAPQV
                     DIMQRFVALGATVQAYDPVAMENAQRVVPDVAYCSNAYEAAAGADAVLLLTEWNEFKQ
                     LDMNRVRTGLRRPLLVDGRNVFVPAEMRGQGFEYVSVGRP"
     gene            complement(1612..2955)
                     /locus_tag="VF276_10675"
     CDS             complement(1612..2955)
                     /locus_tag="VF276_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607365.1"
                     /translation="MSAIAGGSEADREGAHNAAPGPAPAPADARYRQLAWLVMGTGFV
                     IFCLLCAGFVAGSAYYSQHASIVPQTAATVELAHGTKLEVQHAGQKAWNLVSKATELS
                     EGDSVRTGQDTDALLTLFDQSTVQLYYSSTVTLKRIRSSQFLNQFKEMRLVQSSGSIK
                     VAAAALGPYNTMELQVTNPDGKVTVGVPENSVAVITLDTPAVDNAYMTVATRTGTSWV
                     RTTGPRRIIPIGEMCRVQPDYTVTDPGQEEIELVDNSEFVKPADNPNTEIAAGWRLSP
                     LNPAEEQITATRSTETFFGKTVYTANFLRAAQSTELATVRIRQDMDIPVNFYQQLQVK
                     AAVKLVEPPYIGPGLYPLTLRVTYEDLDGVTRLWERNFYAGPLPYPPEGGTIELDAGT
                     WKELPQELPSDGVETAANWDLMTQSPAPARIKAVEVIVTGYSFNVAVSGISLTAR"
     gene            complement(2952..3239)
                     /locus_tag="VF276_10680"
     CDS             complement(2952..3239)
                     /locus_tag="VF276_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX6607366.1"
                     /translation="METEKARLQARVQGRVQGVGYRVFVREAARRLHLAGSVRNEEDG
                     SVLVVAEGARADLERLLAELHRGPVAAWVTRVDVRWDAPGGEPAGRFEVQG"
BASE COUNT          539 a         1175 c         1142 g          600 t
ORIGIN      
        1 gatgcccgtg cccagcgtct tcgagatcgc ttccttcgcc gcgaaccgcg ccgccagctc
       61 gggcgtgcgc ccgcggcaga agcgctgctc gccgggcgtc cagacccggt cgagcagcgg
      121 ctggccccag cgctcgatgg ccgccgcgat gcgcgggatc tcgatcacgt cgatcccgca
      181 ctgcaacagg ccgtcatggg cgtcgtccag gcgcaggccg agggcggcca gccagggcgg
      241 ctcccccgcg aggcggctgg ggctgaactc gcggcgcgcg gtcacggccg gcccacggac
      301 acgtactcaa agccctggcc gcgcatctcc gccgggacga agacgttgcg gccgtcgacc
      361 agcagcgggc ggcgcaggcc ggtgcgcacg cggttcatat cgagttgctt gaactcgttc
      421 cattcggtca gcagcagcac ggcatcggcg cccgcggcgg cctcataagc gttcgagcag
      481 taggccacgt cgggcaccac ccgctgcgcg ttctccatcg ccaccgggtc ataggcctgc
      541 accgtggcgc cgagcgcgac gaaccgctgc atgatgtcca cctgcggcgc ctcgcggatg
      601 tcgtcggtgt tgggcttgaa cgacacgccc cacacgccga tgcgcttacc gtgcaggtcg
      661 ccgccgagca ggttggtcac cttttcgacg aacagccgcc gcgcgtcggt gttgatgtcc
      721 atcaccgcgt tgagcaactg cgggtggcag tcgttggacg ccgccatatg ggccagggcc
      781 ttgacatctt tggggaaaca cgagccgccg tagccgatcc ccgcgtcgag gaactggtgg
      841 ccgatgcgct tgtcgtagcc catgcccagc gccacctgct tcacgtcggc gccgagcgtc
      901 tcgcagatgt gcgcgatctc gttgatgaac gagatgcgcg tcgccaggat ggcgtttgac
      961 gcatacttga tcatctcggc ggtgcggata tcggagatga tgatcggggc gttgagcggc
     1021 tcgtagagcc cagccacttt ctcggacgcc tcgcggtccg acgagcccag caccacacgg
     1081 tcgggcacca tgaagtcgcc cagcgccgcg ccctcgcgca agaactccgg attggacacg
     1141 accgagaact tcaggcccgg cttgatatgc tcgccgacca ggcgcgtcac caggtcgccc
     1201 gtgccgatgg gcacggtgct cttgttgatg acgatcaagt cgttcgaggc atgcttgccg
     1261 atggaccgcg ccgcggactc gacctgcgac atatcggcgg cgccgtccgg cccctccggg
     1321 gtgcccacgg cgatgaagac aaagtcggcg ccgcgcaggc cctcggcgta atcggtcgtg
     1381 aagttcaggc ggcccgcctt gaggttgcgt tgcagcacct cttcgatgcc cggttcatag
     1441 atgggcgaga tgccctggcg caggcgttcc actttttcgg gcacgatctc gatgcccatc
     1501 acctcgttgc ccaggtctgc gaaacaggcc gcggtcacga gacccacata gttgaccccg
     1561 accacactga tcttactcaa gagaagcctc cttgctgaaa caaaacgcgc ctcatcgagc
     1621 ggtgagcgat atgcccgata cggcgacatt aaaactatag ccggtgacga taacctcgac
     1681 cgccttgatg cgggcgggcg cgggtgattg tgtcatcaag tcccaattgg ccgccgtctc
     1741 cacgccgtcg gacgggagtt cctgcggcag ctccttccag gtgccggcat cgagttcgat
     1801 ggtgccccct tccggggggt acggcagcgg cccggcgtag aagttgcgct cccacagccg
     1861 cgtcaccccg tccaggtcct cgtacgtcac gcgcagggtg agcgggtaga ggcccggccc
     1921 gatgtacggc ggctcgacca gcttgacggc ggccttcacc tgcaactgct ggtagaagtt
     1981 aaccgggatg tccatgtcct ggcggatgcg gacggtcgcc agttccgtgc tttgcgcggc
     2041 gcgcaggaag ttggcggtat agaccgtctt gccgaaaaac gtctcggtgc tgcgcgtggc
     2101 ggtgatctgt tcctccgccg ggttgagcgg cgacaggcgc cagccggcgg cgatctccgt
     2161 gttcgggttg tcggcgggct tgacgaactc gctgttgtcg acgagttcga tctcctcctg
     2221 gccggggtcc gtgaccgtgt aatcgggttg gacccggcac atctcgccga tggggatgat
     2281 ccggcgcggc ccggtggtgc gcacccagga cgtgcccgtg cgcgtcgcca cggtcatgta
     2341 ggcgttgtcg acggcagggg tatcgagcgt gatcacggcg acgctgttct ccggcacgcc
     2401 caccgtgacc ttgccgtccg gattggtgac ttgcagttcc atggtgttgt acggccccag
     2461 cgccgccgcc gccactttga tgctgcccga actctgcacc aggcgcattt ccttgaactg
     2521 gttgagaaac tggctgctgc ggatgcgttt cagggtcacg gtgctgctgt agtaaagctg
     2581 gacggtgctc tgatcgaaga gcgtgagcaa ggcgtcggtg tcctggccgg tgcggacgga
     2641 gtcgccttcc gacaactcgg tggctttgga gacgaggttc caggctttct gcccggcatg
     2701 ctggacctcc aacttggtgc cgtgggccag ttcgaccgtg gcggcggtct gcggcacgat
     2761 gctggcgtgc tggctataat aggccgaccc ggccacgaag cccgcgcaca gcaggcaaaa
     2821 gatgacgaag ccggtgccca tgaccagcca ggccagttgc cggtagcgag catcggcggg
     2881 cgccggggcg ggtccggggg cagcgttgtg cgccccttcg cggtccgctt ccgagccgcc
     2941 ggcgatcgca ctcacccctg cacctcaaac cgcccagccg gttccccacc gggcgcatcc
     3001 caacgcacat cgacccgcgt cacccacgcg gcgaccggcc cgcgatgcaa ctcggccagc
     3061 agtcgctcca gatcggcgcg cgctccttcc gcgacgacca gcacgctgcc gtcctcctcg
     3121 ttgcggacgc tgcccgcgag atggagccgg cgcgccgcct cgcgcacaaa tacgcgatag
     3181 cctaccccct ggactcgccc ctgcacacgg gcctggagtc gcgccttctc agtttccata
     3241 gactctctat tgtacccccc agcgggcgct gcgtcaaacg agcccagccg gatcaacctc
     3301 tcccccagcc ccccgccgcg ggaagaggag tcgtcattcc cccttcccgc gccgggaagg
     3361 gggccagggg gttaggcccg ccgcggtcac gccaggcggt gttcaatagc ataacgcgtg
     3421 gcggcgctgc gggtcgtgag gtccagcttg ctgtag
//
LOCUS       DASUYC010000656         2132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1830822, whole genome shotgun sequence.
ACCESSION   DASUYC010000656 DASUYC010000000
VERSION     DASUYC010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1830822"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..1382)
                     /locus_tag="VF276_10685"
     CDS             complement(126..1382)
                     /locus_tag="VF276_10685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6607367.1"
                     /translation="MRVCFLNPQGHLQGHPPLGKTDTGGQIVYVLELARALSRQGIKV
                     DLITRQFDGQKPVEDLTSNARIVRLPCGPDTFVVKEHLYELMNQWVQRIEAFSRTEDV
                     QYDIIHSHYWDGGYAGMLLQRRLHIPHVFTPHSLGRWKELALSHDAVPGQALAPLYRY
                     QLRIMTEKRIMKTADVVLMLSQIQRVRLMQHYRLDFDKIRVLYPGVDTRVFTPNHPAG
                     GNGRMPTQNNILLVSRFVPAKGIDTAIEVFRRVARRVDCHLYIVTTNKLDDFSEEEIA
                     NERAVRWMIDTHRLQDRVHFLGFIDNRRLLADYYRSADLFLLPSRYEPFGLTTLEALA
                     CGAVTLVSHAAGSRELLIDGLNGFIVDMEEPDAVAKLIVSLFKDRALAQRIAENAVLS
                     TQRHFSWDVTAKRLLKYVYPQAALPK"
     gene            complement(1386..>2132)
                     /locus_tag="VF276_10690"
     CDS             complement(1386..>2132)
                     /locus_tag="VF276_10690"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX6607368.1"
                     /translation="VSTEALYRTEAAPTTNVFLTQTTSVPDFQAIRGADALLDIRDVP
                     EELIERARAVHTSAFALSLEPQRSAIRRALRLAVRHKKVVSLDPNYSPRIWPDKEEAW
                     EVLAEIMPTVTIVKPSLEDARRLFDFNLSEDALEERCLREFHDLGAQVVIVTRSGQSV
                     TVSDGKTVERVGPLPRVEVANVTGGRDAFWSALLVAHLEGKPWPVAVRFAHEVAALKL
                     RVLGHVERMIDREEIYRRLADQPAPATTAG"
BASE COUNT          341 a          713 c          713 g          365 t
ORIGIN      
        1 ctgacaggcc agatacgtgg ggcgccgtag cgggatgaac aatgcgttcc acaccggccc
       61 ggtccccgtt cgcgctgtag gggccggttg caaaccggcc cgcgccccgc cggccgcgca
      121 acgcctcact tgggcagcgc ggcttgcggg tagacgtact tgagcagccg cttcgccgtc
      181 acgtcccaac tgaaatggcg ctgggtcgag agcaccgcgt tctcggcgat gcgctgggcc
      241 agggcgcggt ccttgaacag gctgacgatg agcttcgcca cggcgtccgg ctcctccata
      301 tccacgatga agccgttcag cccgtcgatc agcagttcgc ggctgcccgc ggcgtgcgac
      361 accagcgtca ccgcgccgca ggctagcgcc tccagcgtgg tcagcccgaa cggctcgtag
      421 cgcgagggca gcaggaacag gtcggcgctg cggtagtagt cggccagcag cctgcggttg
      481 tcgatgaagc ccaggaagtg gacccggtct tgcaggcggt gcgtgtcgat catccagcgc
      541 acggcgcgct cgttcgcgat ttcctcctct gaaaaatcgt ccagcttgtt cgtcgtgacg
      601 atatacaggt ggcaatccac ccgccgggcc acgcgccgga agacctcgat agccgtatcg
      661 atgcccttgg ccggaacgaa gcgcgacacc aggaggatgt tgttctgtgt gggcatgcgt
      721 ccgttcccgc ccgcgggatg attcggggta aagacgcgcg tgtccacccc cggatagagc
      781 acgcggatct tgtcgaaatc gagccggtaa tgctgcatca gccgcacgcg ctggatctgg
      841 ctgagcatca agacgacatc cgccgtcttc atgatccgct tctcagtcat gatgcgcaac
      901 tggtagcggt agagcggggc cagggcctgg ccgggtacgg cgtcgtggct cagcgccagc
      961 tccttccagc ggcccagcga gtggggcgtg aagacatgcg ggatgtgcag ccgccgttgc
     1021 agcagcatgc ccgcgtagcc gccgtcccag tagtgcgagt gaatgatgtc gtattgcaca
     1081 tcctcggtgc ggctaaaggc ttcgatgcgc tgcacccact ggttcatcaa ctcgtacagg
     1141 tgctctttga ccacgaaggt gtccggcccg cagggcagcc ggacgatgcg cgcgttggag
     1201 gtcaggtctt cgaccggctt ctggccgtcg aactgccgcg tgatcaggtc caccttgatc
     1261 ccctggcggc tcagcgcgcg cgccagttcc agcacgtaga cgatctgccc gccggtgtcg
     1321 gttttgccga gcggggggtg gccttgcagg tggccctgcg gattgagaaa gcagacccgc
     1381 atggctcagc ccgccgtcgt cgcgggcgcg ggctggtccg ccaggcgccg gtagatctct
     1441 tcccggtcga tcatacgctc tacgtggccc agcacccgca gcttgagcgc cgccacttcg
     1501 tgggcgaagc gcacagcgac gggccagggt ttgccttcca ggtgggccac cagcagcgcc
     1561 gaccagaagg cgtcgcgccc gccggtcacg ttggcgacct cgacgcgcgg cagcggcccc
     1621 acccgctcca ccgtcttgcc gtcggacacg gtgacgctct ggccgctgcg ggtcacgatc
     1681 accacctggg cgcccaggtc gtggaactcg cgcaggcagc gctcctccag cgcgtcctcg
     1741 gagaggttga agtcgaacag ccgccgcgca tcctccagcg acggcttgac gatggtcacg
     1801 gtcggcatga tctcggccag cacctcccag gcttcctctt tatcgggcca gatgcgcggg
     1861 ctgtagttcg ggtcgagcga cacaactttc ttgtggcgca cggccaggcg cagcgcgcgc
     1921 cggatcgccg agcgttgcgg ctccagcgac agcgcaaaag ccgacgtatg cacggcccgc
     1981 gcccgctcaa tcaattcctc cggcacgtcc cggatgtcga gcagggcgtc cgccccgcgg
     2041 atggcctgga aatcgggcac gctggttgtc tgcgtgagga agacattggt cgtgggcgcg
     2101 gcctcggtac gatacagcgc ctcggtgctc ac
//
LOCUS       DASUYC010000657         3056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1125985, whole genome shotgun sequence.
ACCESSION   DASUYC010000657 DASUYC010000000
VERSION     DASUYC010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125985"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2071
                     /gene="glgX"
                     /locus_tag="VF276_10695"
     CDS             <1..2071
                     /gene="glgX"
                     /locus_tag="VF276_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEX6607369.1"
                     /translation="GYIPGIQPGQLYGYRVYGPYEPQAGLRYNPNKLLLDPYAKAING
                     TIKWSNALFGYPVDSPDDDRDLEMDTEDSAAGMPKAVVTDPSFFWGNDTPPRTPLHES
                     VIYEMHVKGFTQRHPEIPPELRGTYAGLAAPESIAYLQRLGITAIELQPVHHFVHDKT
                     LRDKGLRNYWGYNTIGYFAPHAEYASAGMTGEQVREFKAMVRTMHAAGIEVILDVVYN
                     HTAEGNHYGPTLSFKGIDNAAYYRLVPGNPRYYMDYTGTGNTLNMLNPRTLQLVMDSL
                     RYWITEMHVDGFRFDLASTLARGLHEVGRLSSFLDTIHQDPIISQVKLIAEPWDVGEG
                     GYQVGNFPVLWAEWNGKYRDTVRRFWRGDESQVQELAYRLSGSSDLYQHNGRRPSASI
                     NFVTAHDGFTLNDLVSFDEKHNEANGENNMDGESHNLSWNCGAEGATDDPAICTLREK
                     QKRNFLATLLLSQGVPMIHAGDECGRSQGGNNNAYCQDNEISWFDWEWDDADLALFDF
                     TRRLIAIRRAYPVLHRRKFFQGRSIHGTDVRDIRWISPGGSDMTDEEWANGRVHVLGM
                     LLNGAVMDESNHKGENIKDDILLMLVNGSWEDVPFRLPGKRKDPSWDVLVHTATPNVV
                     VGKAIPCGQQLPLPARSLVLLHQPRPEPEQPPRAEAEVWRPGRGAAAVPPEPNTNVSS
                     ELRRSNS"
     gene            2068..>3056
                     /locus_tag="VF276_10700"
     CDS             2068..>3056
                     /locus_tag="VF276_10700"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX6607370.1"
                     /translation="MTWQLPIGAQLQDDGVHFRVWAPDRRQVEVAILDDHDHEAGRHP
                     LTRDAGGYFAGTVAGLGAGSRYLYVLDGEVRRPDPASRYQPEGVHGPSAVVDPGFGWT
                     DQGWQGRPLEEVIIYEVHIGTATSSGTFESFIERLPYLQSLGVTTIEIMPVADFPGTR
                     NWGYDGVSLFAPARAYGGPDGLKRLVDAAHAQGLAVFQDVVYNHLGPDGNYLRDFSAH
                     YFTSGEQTPWGEAIDFSRPEVRAFFIANALYWAHEYHMDGLRLDATHALLEAGRKQML
                     RELTEQVRASLPAGRNFLLCAEDDRNDTWLITPGARGGAGMDAIWADDFHHEI"
BASE COUNT          571 a         1082 c          945 g          458 t
ORIGIN      
        1 cggctatatc cccggtatcc agcccggcca gttgtacggc tatcgcgtct acggccctta
       61 tgagccgcag gccgggctgc gctacaatcc caataaactg ctgctggacc cgtatgccaa
      121 ggccatcaac ggcacgatca agtggagcaa cgccctcttc ggctacccgg tggatagtcc
      181 cgatgacgac cgcgacctgg agatggacac cgaggacagc gcggcgggga tgcccaaagc
      241 cgtcgtcacc gacccctcct tcttctgggg caacgacacg ccgccgcgca cgccgctgca
      301 cgagtcggtc atctacgaga tgcacgtcaa aggcttcacg cagcgccacc cggagatccc
      361 gcccgagttg cgcggcacct acgccgggct ggcggcccca gaatcgatcg cctacctgca
      421 acggctgggc atcaccgcca tcgagttgca gccggtgcac catttcgtcc acgacaagac
      481 cttgcgcgac aaggggctgc gcaattactg gggctacaac accatcggct acttcgcgcc
      541 ccacgccgag tatgcgagcg ccggcatgac gggtgagcag gtgcgcgagt tcaaggccat
      601 ggtccgcacc atgcacgccg ccgggatcga ggtcatcctc gacgtggtct ataatcatac
      661 cgccgagggc aaccactacg gccccaccct cagcttcaag ggcatcgaca acgccgccta
      721 ctaccggctg gtgcccggca acccgcgcta ctacatggat tacaccggca ccggcaacac
      781 gctcaacatg ctcaacccgc gcaccctgca actggtgatg gacagcctgc gctactggat
      841 caccgaaatg catgtggacg gcttccggtt cgacctggcc tccaccctgg cccgcgggct
      901 gcacgaggtg ggccgcctgt ccagcttcct tgacaccatc catcaggacc cgattatctc
      961 gcaggtcaag ctgatcgccg agccgtggga cgtgggcgag ggcggctacc aggtcggcaa
     1021 cttcccggtg ctgtgggccg agtggaacgg caagtaccgc gacaccgtgc gccgcttctg
     1081 gcgcggcgac gaaagccagg tgcaggaact ggcctaccgc ctcagcggca gcagcgacct
     1141 ctaccagcac aacggtcgcc gcccctcggc cagcatcaac ttcgtcaccg cccacgacgg
     1201 cttcacgctc aacgatctcg tctccttcga cgaaaagcac aacgaggcca acggcgaaaa
     1261 caacatggac ggcgagagcc acaacctgag ctggaattgc ggcgccgaag gcgcgaccga
     1321 cgaccccgcg atctgcaccc tgcgcgagaa gcagaaacgc aacttcctgg cgaccctgtt
     1381 gctcagccag ggggtgccga tgatccacgc cggggacgag tgtggccgca gccagggcgg
     1441 caacaacaat gcctattgcc aggataacga gattagctgg ttcgactggg agtgggacga
     1501 cgccgacctg gccctgttcg acttcacgcg ccggctcatc gccatccggc gcgcgtaccc
     1561 ggtgctgcac cgccgcaagt tcttccaggg ccgcagcatc cacggcaccg atgtccgcga
     1621 catccgctgg atcagtcccg gcggatcgga catgaccgac gaagagtggg ccaacggccg
     1681 cgtccacgtc ctgggtatgc tgctcaacgg cgcggtcatg gacgaatcaa atcacaaggg
     1741 cgaaaatatc aaggatgata tcttgctgat gctggtcaat ggatcctggg aggatgtgcc
     1801 gttccggctg ccgggcaagc gcaaggaccc gagttgggac gtgctggtcc atacggccac
     1861 acccaatgtg gttgtgggca aggcgatacc gtgcgggcag caactccccc tcccggcccg
     1921 ctcgctggtc ctgctgcacc agccgcgccc ggagcccgag caaccgccca gggccgaggc
     1981 cgaggtctgg cggccagggc gtggcgcggc cgccgtgccg cccgaaccga ataccaacgt
     2041 ctctagtgaa cttagaagga gcaactcatg acctggcaac tgccgatcgg cgcccaactc
     2101 caggatgatg gcgtgcattt ccgcgtctgg gcgccggatc gccggcaagt cgaggtcgcc
     2161 atcctggacg accatgacca cgaagcaggc cgccatccgc tgacccgcga cgccggcggc
     2221 tatttcgcgg gcaccgttgc gggcctgggg gccgggagcc gctacctcta cgtcctggac
     2281 ggcgaggtcc gccgccccga tccggccagc cgctaccagc cggagggcgt ccatggcccc
     2341 agcgccgtgg tggaccccgg cttcggctgg accgaccagg gctggcaggg gcgcccgctg
     2401 gaagaggtca tcatctacga ggtccatatc ggcacggcca catccagcgg cacattcgag
     2461 tcgttcatcg agcgcctgcc ctatctccag tcgctcggcg tgaccaccat cgagatcatg
     2521 cccgtggccg acttccccgg cacgcgcaac tggggctacg acggcgtcag cctgttcgcc
     2581 ccggcccgcg cctatggcgg gcccgacggc ctgaagcggc tggtcgacgc cgctcacgcg
     2641 cagggcctgg ccgtcttcca ggatgtggtc tacaaccacc tggggcccga cggcaactac
     2701 ctgcgcgact tcagcgcgca ctactttacg tccggcgagc agacgccctg gggcgaggcg
     2761 atcgacttca gccggcccga agtgcgcgcc ttcttcatcg ccaatgccct ctactgggcg
     2821 cacgagtacc acatggacgg cctgcgcctc gatgcgactc acgccctgct cgaagcgggc
     2881 cgcaagcaga tgttgcgcga gctaaccgag caggtgcggg ccagcctgcc cgccgggcgg
     2941 aacttcctgc tctgtgccga ggacgaccgc aacgacacct ggctgatcac ccccggcgcc
     3001 aggggcggcg cgggcatgga cgccatctgg gccgacgact tccaccacga gatcca
//
LOCUS       DASUYC010000658         2060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_2058927, whole genome shotgun sequence.
ACCESSION   DASUYC010000658 DASUYC010000000
VERSION     DASUYC010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2058927"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1610)
                     /locus_tag="VF276_10705"
     CDS             complement(<1..1610)
                     /locus_tag="VF276_10705"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6607371.1"
                     /translation="MTRHPAASRMARRRPVARRAPGLAALLIGLVTLAGCAWPWEPAP
                     TPTPTAPATATAPAAWAPTLPTATMPASPVAQAGHFVDADQGIAIDYPTTWKRQASQD
                     PRILTWLLVPGQGVGIGIYASALTAGATLEQAAGQVRDLSTGGLTAITDRPAHALTLA
                     DGRPAWVVEYDAKESDGSALRAVLITTTRGGQVAALIAFGAPDDIEAVRPALTQAATQ
                     LKLTPPQVAGVPREDALVRVGGESNNPRDYDPATMDGHSPIFSGLVAFNPQLQVAPDL
                     AESWDLDATGTVYTFHLRANARFHNGRPVTAQDVIYSWERAASPAVDSHTVLTYLGDI
                     VGIGARRAGQAEHISGLRALDDHTLQVTIDAPKPYFLMKLTYTTAAIVDRANVESGPD
                     WYRTPNGTGPYRLIRWEPGKLELLERFDGFYLPPPPIRYLVTQLYAGVGTRLYETGAI
                     DMTSVSRADVARMRDPAEPLHAEFHESASMCTSYLSFDVTQPPFDDPKVRQAFALAVD
                     RQRYIDIVLRGAAIPAHGLYPPALPGYNP"
     gene            complement(1607..>2060)
                     /locus_tag="VF276_10710"
     CDS             complement(1607..>2060)
                     /locus_tag="VF276_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607372.1"
                     /translation="NYLVSTFGPDRMRALFAALHDGRTIEEALQATYHLNLAGLEAGW
                     RQSIGARPPAAAVGTAIASATPVPTYPPIEAAPSSASAALAAPTARPTARPAAPAAVA
                     AGDSGIAAAGAGTGPDAGALILGLAVVLCIGGLVTGLLRLRRQRKRTL"
BASE COUNT          266 a          676 c          832 g          286 t
ORIGIN      
        1 ggattgtagc cgggcagcgc gggcggatac agcccgtgcg ccgggatggc cgctccgcgc
       61 agcacgatgt cgatgtagcg ttgccggtcc acggccagcg cgaacgcctg gcgcaccttg
      121 gggtcgtcga acggcggctg cgtcacgtcg aaactgaggt acgacgtaca catgctcgcg
      181 ctttcgtgga actcggcgtg cagcggttcc gccgggtcgc gcatgcgcgc cacgtcggcg
      241 cgactcacgc tcgtcatgtc gatggcgccg gtttcgtaga gccgcgtgcc caccccggcg
      301 tacaactgcg tcaccaggta gcggatcggc gggggcggca ggtagaagcc gtcgaagcgc
      361 tccagcagtt cgagcttgcc gggctcccag cggatcagcc ggtaggggcc ggtgccgttg
      421 ggcgtacgat accagtccgg ccccgactcg acgttagccc ggtcgacgat ggccgccgtg
      481 gtgtaggtca gcttcatcag gaagtaaggc ttgggcgcgt cgatggtcac ttgcagcgta
      541 tggtcgtcga gcgcgcgcag gccgctgatg tgctcggcct gacccgcccg ccgcgcgccg
      601 atgccgacga tgtcgcccag gtaggtcagc accgtgtgcg agtccacggc gggcgatgcg
      661 gcgcgttccc aggagtagat cacgtcttgc gccgtgaccg ggcgcccgtt atggaagcgg
      721 gcattggcgc gcaggtggaa ggtatagacc gtgccggtgg cgtccaggtc ccaggactcg
      781 gccaggtcag gggccacctg gagttgggga ttgaaggcga ccaggccgct gaagatgggc
      841 gagtggccgt ccatggtggc cgggtcgtaa tcgcgcgggt tgttggactc gccgccgacg
      901 cgcaccagcg cgtcctcgcg gggcacgccg gccacctgtg gcggggtcag cttaagttgc
      961 gtggcggcct gggttaacgc ggggcgcacc gcctcgatgt cgtccggggc gccgaaggcg
     1021 atcagggcgg cgacctgccc gccgcgggtg gtggtgatca gcacggcgcg cagggcgctg
     1081 ccgtcgctct ccttggcgtc atactcaacg acccaggcgg ggcggccatc ggccagggtg
     1141 agggcgtggg cgggccggtc ggtgatggcg gtcagcccgc cggtgctcag gtcgcgcacc
     1201 tggccggctg cctgctccag cgtggcgccc gcggtgagcg cgctggcata gatgccgata
     1261 cccacgccct ggcccggcac cagcagccag gtgaggatgc gcgggtcctg gctggcctgg
     1321 cgcttccagg tggttgggta gtcgatcgcg atgccctggt cggcgtccac gaaatgcccg
     1381 gcctgggcca cgggcgaggc cggcatcgtg gccgtgggta gcgtgggcgc ccaggcggcg
     1441 ggcgcggtgg ccgtggccgg cgcggtgggc gtgggcgtgg gcgccggctc ccagggccag
     1501 gcgcagccgg cgagcgtcac gaggccgatc agcagggcgg cgaggcccgg cgcccgccgg
     1561 gcgaccgggc gacggcgggc catgcgcgag gcggcgggat gacgggtcat aaagtcctct
     1621 ttcgctgccg gcgcaggcgc agcaggccgg tcaccagccc gccgatgcag agcaccacgg
     1681 ccaggcccag gatgagcgcg ccagcgtccg gacccgtccc ggcccccgcc gccgcgatcc
     1741 cggaatcccc ggcggcgacg gcggcgggcg cggcggggcg cgcagtaggc cgcgcggtgg
     1801 gggcggccag ggcggcggag gcgctggagg gcgcggcctc gatgggcggg taggtgggca
     1861 ccggggtcgc gctggcgatg gcggtgccca ccgcagcggc cggtgggcgc gcgccgatgg
     1921 actggcgcca cccggcctcc agcccggcca ggttcaggtg ataggtggcc tggagcgcct
     1981 cctcgatggt ccgaccgtcg tgcagcgcgg cgaacagcgc ccgcatccgg tcggggccaa
     2041 aggtgctgac caggtagttc
//
LOCUS       DASUYC010000659         3695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1631316, whole genome shotgun sequence.
ACCESSION   DASUYC010000659 DASUYC010000000
VERSION     DASUYC010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1631316"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="VF276_10715"
     CDS             <1..931
                     /locus_tag="VF276_10715"
                     /inference="COORDINATES: protein motif:HMM:NF013252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607373.1"
                     /translation="VRAQAHKAGDRVELYSRTLDPVTHRFPEIVAALAALPGDFVLDG
                     EAVAYDAEAGRCLPFAALQKRLGRKTVTPALLKSTPVAFMAFDLLYQDGESLLHLPLV
                     ERRVRLDALIGAGSPALVPGIQAHVLLADLTAPRHEPAELDALFDAARRRGNEGLLVK
                     DPTSPYSPGRRGRAWLKVKKALATLDVVVTAVEWGQGRRRHLLSDYTFAVRDGDRLLN
                     VGKAYSGLTDAELAEMTEWFKAHTLRDFVAGRVVEPQIVIEVAFDIVQPSPRHKSGYA
                     LRFPRIVRLRPDKPVSEINTLDDVRALAAPATP"
     gene            1047..2072
                     /locus_tag="VF276_10720"
     CDS             1047..2072
                     /locus_tag="VF276_10720"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX6607374.1"
                     /translation="MQPVYRVSRARKALIGLLSCYPLLVLGLAAIHLLAPQRSGALAL
                     SQIFAPYLFVPLPALLPFALLRGAGPLRVLLLLCALVFGVRYAPRLVPTATAAPPGAT
                     QVEVMNWNVRLGGQWDQVRPVLLSRPADVITLEEITWRRLVDDKMLVQLYPYHVVYQP
                     AYGNSEVLLSAYPILDQGALDPEGTEGPPPRMVWARLDLGEGRTLVVVGAHPYTAKIN
                     LRACPRTRCYDPSTRDAQIARIRAFFEPILQQGEPLIVSGDFNVTEREPAYADLARGL
                     LAVQRQVGWGLGLSWGMDPTKNQPIPWLRIDHMFSSPNVTPVRTSVDCTLRGSDHCIV
                     HGTFAVP"
     gene            complement(2153..>3695)
                     /locus_tag="VF276_10725"
     CDS             complement(2153..>3695)
                     /locus_tag="VF276_10725"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEX6607375.1"
                     /translation="THIEFVYGTISTPPPPAASPSKKAARPAAAGGDYATIGVQQGAN
                     SRVTQYAYDQPGSINDNLVLSFDFVPVLDPCLDYQVGTGSGQLDPGVTDIGNHGDDVT
                     TTVTLPFAYTLYDQAFTTVTAGSNGVLEFVSSDNSPDNTNLPAAKFNYAILPYWDNLD
                     TWGGGDCPECGIFTSVTGTAPNRIFNVEWRVIDPAFNHYNFEVRLFENQTMFQVVYGS
                     LPSPPPPRPLGPAGDSATIGVQKDTGSRYTLYAYNEPGSVDDNMVLTFSLGCVPTATP
                     TSPPAATATATPVPPGTATATPVPPTATVPPAATGTPCSITFRDVPAEDWSNGYIRWA
                     YCAGIVNGYADNTFRPNAETTRGQIAKMVVLAAGWPLVVAPGAPHFSDVPPAHPVYTY
                     IEVARVHGAISGYDDGTFRPWNVVTRAQLVKILVLMRGYQILTPATPTFTDVGPDYWA
                     YGYIETAYHHALIGGYDCGPVVPTPPIDPNACREFRPDRPATRAQLSKILFQAYVVPS
                     NKSEP"
BASE COUNT          534 a         1221 c         1333 g          607 t
ORIGIN      
        1 cgtgcgcgcc caggcccaca aggcgggcga ccgcgtggaa ctctactcgc gcacgctcga
       61 cccggtgacg caccgcttcc cggagatcgt ggccgcgctg gccgcgttgc ccggcgactt
      121 tgtgctcgac ggcgaggccg tggcctacga cgccgaggcc gggcgctgcc tgcctttcgc
      181 cgcgctgcaa aagcgcctgg gccgcaagac ggtcacgccc gccctgttaa agagtacgcc
      241 cgtcgccttc atggcgttcg acctgctcta ccaggacggc gaatcgctgc tgcacctgcc
      301 gctggtcgag cgccgcgtcc ggctcgacgc actgatcggg gccgggagtc cggctctggt
      361 gcccggcatc caggcgcacg tgctcctggc cgatctgacg gcgccgcggc acgaacccgc
      421 cgaactcgat gcgctgttcg acgcggcgcg ccgccggggc aacgaggggt tgctggtcaa
      481 agaccccacc tcgccctaca gccccggtcg ccggggccgc gcctggctca aggtgaagaa
      541 ggcgctggcg accctcgacg tggtcgtgac cgccgtggag tggggccagg gccgccgccg
      601 ccacctgctc agcgactaca ccttcgccgt gcgcgacggc gaccggttgc tcaacgtggg
      661 caaagcctac tccggcctca ccgacgccga actggccgag atgaccgagt ggttcaaggc
      721 ccacaccctg cgcgacttcg tggccgggcg ggtggttgag ccgcagatcg tcatcgaggt
      781 ggccttcgac atcgtgcaac ccagcccgcg ccacaagagc ggctacgccc tgcgcttccc
      841 gcgcatcgtc cgcctgcgcc ccgacaagcc agtcagcgag attaacacgc tggacgacgt
      901 gcgtgcgctg gccgcgcccg ccacgccctg accgcgccgc ccctgccccg tcgcgcctcg
      961 ccccacgcct gagttgtaac cttcccggtg tccggtccgt tgatagagag agacgaatcg
     1021 acggaggaaa aggacacaat tcgcgtatgc aacctgttta tcgggtgagc cgggcgcgca
     1081 aggcgctcat cggcttgctg tcgtgctatc cgctgctggt gctgggcctg gcggcgatcc
     1141 acctgctggc gccgcaacgc agcggcgccc tggcgctgag ccagatcttc gcgccttacc
     1201 tgttcgtgcc tctgccggcg ctgttgccgt tcgcgttgtt gcgcggggcc gggccgctgc
     1261 gcgtgctgtt gctgctgtgc gcgctggtgt tcggcgtccg ctacgcgccg cgcctggtgc
     1321 ccaccgccac ggcggcgccg ccgggcgcca cgcaggtcga ggtgatgaac tggaacgtgc
     1381 gcctgggcgg gcagtgggac caggtgcgcc cggtgctgct gtcccggccc gccgatgtga
     1441 tcaccctgga ggaaatcacc tggcgccggc tggtagatga taagatgctg gtccagctct
     1501 atccctacca tgtggtctat caaccggcct acggcaacag cgaggtgctg ctgagcgcct
     1561 acccgatcct cgaccagggt gcgctggacc cggaagggac ggaaggcccg ccgccgcgca
     1621 tggtctgggc acggctcgat ctgggcgagg gccggacgct cgtcgtagtg ggcgcccacc
     1681 cctacacggc gaagatcaac ctgcgcgcct gcccgcgcac ccgctgctac gatccgtcca
     1741 cgcgcgacgc gcagattgcg cgcatccgcg cctttttcga gccgatcttg cagcaaggtg
     1801 agccgctgat tgtctctggc gacttcaacg tgaccgagcg cgaaccggcc tacgccgacc
     1861 tagcgcgcgg cctgctggcg gtgcagcgcc aggtgggctg gggcctcggc ttgtcgtggg
     1921 gcatggaccc gaccaaaaac cagcccatcc cctggctgcg tatcgaccac atgttcagca
     1981 gccctaacgt cacgccggtg cgcaccagcg tcgattgcac cctacgcggc agcgaccact
     2041 gcatcgtcca cggcaccttc gccgtgccct gacccggcga gccgccgggc agacaaaaag
     2101 ccggacccgc gcgggtccgg cttttcgcgt tgggtgccgt gcggcggctt gtctaaggct
     2161 cgctcttgtt cgagggcacg acgtaggcct ggaagaggat cttgctgagt tgtgcccgcg
     2221 tggccgggcg atcgggccgg aactcgcggc aggcgttggg atcgatgggc ggggtcggca
     2281 cgacggggcc gcagtcgtag ccgccgatca gggcgtggtg gtaggccgtc tcgatgtagc
     2341 cgtaggccca gtagtcgggg cccacgtcgg tgaaggtggg cgtggccggg gtcaggatct
     2401 ggtagccgcg catcagcacc aggatcttga ccaactgagc gcgggtgacg acgttccagg
     2461 gccggaaggt gccgtcgtcg tagccgctga tcgcgccgtg cacgcgggcg acctcgatgt
     2521 aggtgtagac cgggtgcgcg ggcggcacat cgctgaagtg cggggcgccg ggggcgacga
     2581 ccagcggcca cccggcggcc aggacgacca tcttggcgat ctggccgcgg gtggtctcgg
     2641 cgttgggccg gaaggtgttg tcggcatagc cgttgacgat gcccgcgcag taagcccagc
     2701 ggatatagcc gttggaccag tcctcggcgg gcacatcgcg gaaggtgatg gaacagggcg
     2761 tgccggtcgc cgccgggggc acggtggccg tgggcgggac aggggtggcc gtggcggtgc
     2821 cgggcggcac cggggtggcg gtggccgtgg cggcgggcgg gctggtcggc gtggccgtgg
     2881 ggacgcaacc caggctgaag gtcagcacca tgttgtcgtc cacgctacct ggctcattgt
     2941 aggcatacag cgtataccgg ctaccggtgt ccttctgcac gccgatggtc gcgctgtcgc
     3001 ccgccgggcc gagcgggcgg ggcggcggcg ggctaggcag gctgccgtag acgacctgga
     3061 acatcgtctg gttctcgaac aggcggacct cgaagttgta gtgattaaag gcggggtcga
     3121 tgacccgcca ttcgacgttg aagatccggt taggcgccgt gcccgtgacc gacgtgaaga
     3181 tgccgcactc cggacaatcg cctccgcccc aggtgtccag gttgtcccag tagggcagga
     3241 tggcatagtt gaacttggcc gccggcaggt tggtgttgtc ggggctgttg tcgctactga
     3301 cgaattcaag cacgccgttg gacccggccg tcacggtggt gaacgcctgg tcgtacaggg
     3361 tataggcgaa gggcagagtg acggtcgtcg tcacgtcgtc gccgtggttg ccgatgtcgg
     3421 tgacgccggg gtccagttgg ccgctgccgg tgccgacctg atagtccagg cagggatcca
     3481 gcaccggcac gaaatcgaag ctcaacacca ggttatcgtt gatgctgccc ggctggtcgt
     3541 aggcgtactg cgtgacccgg ctgttggcgc cctgctgtac gccgatggtg gcgtagtcgc
     3601 ccccggcagc ggcgggccgg gcggctttct tggacggcga cgcggcgggc ggcggcgggg
     3661 tcgagatcgt gccgtagacg aactcgatat gcgtg
//
LOCUS       DASUYC010000660         8394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_318036,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000660 DASUYC010000000
VERSION     DASUYC010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 8394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8394
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318036"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3163
                     /locus_tag="VF276_10730"
     CDS             <1..3163
                     /locus_tag="VF276_10730"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX6607376.1"
                     /translation="LTAPSSGAVYLVGATVAIAANASDTDGTVARVEFWVDGGKIGED
                     TQSPYTYNWTATAGAHGIQARAVDDLGAITTTSSLPITGNVAPTVGLASPANNSVVAI
                     GSTVPMTANANDPDGSIASVEFWVDGIKVGQDASSPYAWNWTATTGVHALQAKAVDDR
                     GARTGSSIVTITGDVLPTAALTSPANGAIYRVGTAVPMAANASDSDGNVVSVEFWVDG
                     AKVADDTSSPYAYNWTATAGPHTLQAKAVDNVNGRGGSGTVGITGNIGPKVTLTSPAD
                     GTRYSATDTVSMTATASDADGTITRVEFWVDGVDVGNDTSSPYAWTWAPTIGDHTVQA
                     KTQDNNGARTGAPIITIHVAAAPITRTYTYNAYQELCGVDEPETGTTLMGYDAAGNLA
                     WSASGLPSGTACDLEGDTAAILARKAARTYDARNRISTLSFPDLNGNQSWSYTPDGLP
                     ASVTTYNDGGASSVVNSYLYNKRRLPVSETQQVDSLTSSVGYGYDVNGHLSALTYPDN
                     LTVAYAPNALGQPTQAGSYATGVTYYPNGAIEQFTYGNGIVHTLTQNARGLPDRSQDL
                     NGTTSVHDDSYDYDQVGNVLAISDAVSGNRGDRDMVYDDLNRLTSATSPMFGTATYTY
                     DVVDNLRHVTVTGGTQPRDQDYCYDAANRLTNIKTADATYCGGATVVGLGYDVQGNLA
                     NKSGVTYTFDYGNRMRAGGPETYRYDVQGRRVRSNSSAGLIYSLYAQSGQLLFQRDER
                     SGKRRQYVYLGGSLVAESDVPLAGGTATVTYQHTDALGTPVAITNSSRTVTERREYEP
                     YGYQTTPALQDGPNYTGHVADAATGLIYMQARYCDPMIGRCLSVDPVTAYGSGDTRFF
                     NRYAYAFNSPYSFKDPDGRDPKTLDDPRRPIPPPTGPFPCTEDCNPPPPPPQQQSKPP
                     CSEACMRLRYTSSHGGSKSQANSAIQNHQSQSQHGASGSWSAGAEGSWGGNQNQGAKY
                     GIAFGAIGGGVAGSWVVAGLLAPETGGASVGVEFLTLLFELGEPTATIVGAGGLPGAA
                     VGATAGATAGTVIDQSNDDDKDK"
     gene            3165..3428
                     /locus_tag="VF276_10735"
     CDS             3165..3428
                     /locus_tag="VF276_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607377.1"
                     /translation="MNIFPARCFKTPISLLVSTVAAFEICCALPFWAFLVIQWSGNET
                     LSAHRALYSGVVIFGTFFLAALIFWVAIAEPLRKERNQRQKEK"
     gene            complement(3487..3873)
                     /locus_tag="VF276_10740"
     CDS             complement(3487..3873)
                     /locus_tag="VF276_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX6607378.1"
                     /translation="MLDRPNDTAAPAMKDQEFYQRLGRRIAEHRKALGLTQTDLAQEL
                     GVAQQTMAHYEGGTARIAAAMLPTVARVLGVAIEELIGMPARRSVGKRGPAPKLQQQL
                     ERVQSLPKAQQQAISKVLDSVLAAHQ"
     gene            3984..5825
                     /locus_tag="VF276_10745"
     CDS             3984..5825
                     /locus_tag="VF276_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607379.1"
                     /translation="MPQALDPSSALAAPLAAPQAAGVQIAGEDDIRLQRGPRSYRVRG
                     LARNLSAEALKVTLRLSAGEHLHLDTLDLYQARARSAFAKAAALELGVAESVIAGDLA
                     ALVIALEPLQEAAIRGALQPDTSRDAPTLTEAQEAAGLALLRSPDLAERIVADVEAIG
                     VVGEATNALVGYLAMVSRLLAKPLAILIQSTSAAGKSTLMDALLSLLPEGQRVHYSAM
                     TGQSLFYLGEKDLQHKVLAIAEEEGVRQAAYALKLLQSQGELTIASTGKDPQTGKLVT
                     AEYRVEGPVMLCLTTTAIDIDEELLNRCLVLTINETAEQTAAIQQRQRSARTLAGLQA
                     QVRSDHVLTAHRAAQTLLRPLAVVNPFAESLTFASDRVRLRRDHVKYLALIDAIALLH
                     QHQRTVRTVEVDGAGVEYIEVVQSDIALANRLAHAVLGRSLDELPPQTRRVLAALDAW
                     VAAQVGDTDTPRGDVRFTRRTVRGHLGLSDTQLRVHLERLVTLDYVLAHAGKQGQQYA
                     YSLRFDGDAGSDAAQLAGLSVLGVAETEASKPTSRGKTATSRGSDPDFAGGSRGVRGG
                     VAAPSRGAGSSKKPLKTAANGRAAALAPESARRRAPAHRRGNGATAP"
     gene            5825..6832
                     /gene="xerC"
                     /locus_tag="VF276_10750"
     CDS             5825..6832
                     /gene="xerC"
                     /locus_tag="VF276_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006963214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerC"
                     /protein_id="HEX6607380.1"
                     /translation="MAGKTTQRIQWLLGPVAPDSVAAWVRRYLEALETQHRSRSVRKA
                     CRARLAHFNAWCVERGIERPEQVTHATMARFQRHLFVYRKADGQPLAINGQVVTLQTL
                     QAFFRWLVRHGHLPWNPAADLELPRRSDELREPLTLEEVEAVLALPDLADPLGLRDRA
                     MLEVLYATGIRRAELLVLIVSDIDRSRGTLHVRLGKGRRDRFVPVGERALAWIAKYER
                     EARPVLLADPAEVRLFVNYAGVPLTAHSLTWRVRKYFLQAGITKRGACHLFRHTMATA
                     MLDNGADIRHVQEMLGHRQLTTTQRYTHVSIARLKAVHAATHPAANLARREREALEAP
                     D"
     gene            7747..8046
                     /locus_tag="VF276_10755"
     CDS             7747..8046
                     /locus_tag="VF276_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607381.1"
                     /translation="MLRTPGHGMWVRRCAARAFGRIVGCAWRALRTFGQAIGHAPVSR
                     EEEGRRRRRRCPGRDRSALCIARSATVGALPRHPVAASITFLLTWWRPAPTIDPG"
     gene            8168..>8394
                     /locus_tag="VF276_10760"
     CDS             8168..>8394
                     /locus_tag="VF276_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011051193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607382.1"
                     /translation="MSGRVATTKPRRIKASHRRRWKRAAAHREYQHTDALGTPVAITN
                     SSRTVTERREYEPYGYQTTPALQDGPNYTGHV"
BASE COUNT         1479 a         2707 c         2932 g         1276 t
ORIGIN      
        1 cctgaccgca ccatcaagcg gcgcggtcta tctcgtcggc gccacggtgg cgatcgcggc
       61 gaacgccagc gatacggacg gcaccgtcgc ccgcgtcgag ttctgggtcg atggcggcaa
      121 gatcggcgag gacacccaaa gcccatatac gtacaactgg acggcgaccg caggcgccca
      181 cggcatccag gccagggccg tggacgatct gggtgcgatc accacgacat cgtcgctccc
      241 gatcaccggc aacgtcgcgc cgacggtcgg cctggccagt ccggccaaca acagtgtcgt
      301 cgccatcggg tcgacggtgc cgatgacggc gaatgccaac gatcccgacg gcagcatcgc
      361 cagcgtcgag ttctgggtcg atgggatcaa ggtcggccag gacgcgagca gtccctatgc
      421 ctggaactgg accgccacga ccggcgtgca tgccctccag gccaaggcag tcgacgaccg
      481 cggcgccagg accggctcgt ccatcgtcac gatcacgggt gacgtgctgc ccacggctgc
      541 cctgacgtca ccggccaacg gcgccatcta tcgcgtcggc accgccgtgc cgatggcggc
      601 gaacgccagc gacagcgacg gcaacgtcgt tagcgtcgag ttctgggtcg acggcgccaa
      661 ggtcgccgac gacacgagca gcccgtacgc gtacaactgg acggcaacgg ccggccccca
      721 caccctccag gccaaggcgg tcgacaacgt caacggcagg ggaggcagcg ggaccgtggg
      781 catcaccggg aacatcgggc cgaaggtcac gctgacgtcg cccgccgacg gcacccgcta
      841 ttcggcgact gacacggtat ccatgacggc gaccgccagc gacgccgacg gcacgatcac
      901 acgtgtcgaa ttctgggtgg atggcgtcga cgtcgggaac gacacgagca gtccgtacgc
      961 atggacctgg gcgcctacca tcggcgacca caccgtgcag gccaagacgc aggacaacaa
     1021 cggcgcacgg accggcgcac cgatcatcac gatccacgtc gccgcagcac cgatcacgcg
     1081 cacttacacc tacaacgcct accaggaact gtgcggcgtc gacgagcccg aaaccggcac
     1141 cacgctgatg ggctacgacg ccgccggcaa cctggcctgg agcgccagcg gcctgccgtc
     1201 gggcacggcc tgcgacctcg agggcgacac cgccgccatc ctggcccgca aggcggcgcg
     1261 cacctacgac gcccgcaacc gcatcagcac gctgtcgttc ccggacctca acggcaacca
     1321 gagctggagc tacacgcccg acggcctgcc ggcgagcgtg accacgtaca acgacggcgg
     1381 ggccagcagc gtcgtcaaca gctacctgta caacaagcgc cgcctgcccg tcagcgagac
     1441 ccagcaggtc gacagcttga cctcgtcggt gggctacggc tacgacgtca acggccacct
     1501 gtcggcgctc acctatcccg acaacctgac ggtggcctac gccccgaatg ccctgggcca
     1561 gccgacgcag gcgggcagct acgccaccgg ggtgacgtac taccccaacg gcgcgatcga
     1621 gcagttcacc tacggcaacg gcatcgtcca cacgctgacg cagaacgccc gcggcctgcc
     1681 ggaccgcagc caggacctga acggcaccac ctcggtccac gacgacagct acgactacga
     1741 ccaggtcggc aacgtgctgg cgatcagcga tgcggtgtcg gggaatcgcg gcgaccggga
     1801 catggtgtat gacgacctga accggctgac gagtgcgacc tcgccgatgt tcggcacggc
     1861 gacgtacacc tacgacgtgg tggacaacct gcggcacgtg accgtcaccg gcgggacgca
     1921 gccccgcgac caggactact gctacgacgc ggccaaccgc ctgaccaaca tcaagaccgc
     1981 cgatgcgacg tactgcggcg gcgccacggt ggtgggcctg ggctacgacg tgcagggcaa
     2041 cttggccaac aagagtggcg tgacctacac gttcgactac ggcaaccgga tgcgtgctgg
     2101 cggcccggag acctaccgtt acgacgtgca gggacgacgc gtgcggtcca acagcagcgc
     2161 gggcttgatc tacagcctgt acgcccagtc ggggcaacta ctgttccagc gtgacgagcg
     2221 cagcggcaag cgccggcagt acgtgtacct gggtgggagc ctggtggccg agagcgatgt
     2281 gccgctggcc ggcggcaccg cgacggtcac ctaccagcac accgatgccc tggggacgcc
     2341 ggtggcgatt accaacagca gccgcacggt gacggagcgg cgggagtacg agccttacgg
     2401 ttaccagacg acgccggcgt tgcaggacgg gccgaactac acggggcatg tggcggatgc
     2461 ggcgaccggg ttgatctaca tgcaggcccg ctattgcgac ccgatgatcg ggcggtgcct
     2521 gagcgttgat ccggttactg cctatggcag cggcgatacg cggttcttca accgctatgc
     2581 ctatgcgttc aattcgcctt attcgttcaa ggatccggat gggcgtgatc caaaaaccct
     2641 ggatgatccg cgccgtccga ttccgcctcc tactggacca tttccttgta cggaggattg
     2701 caatcctcct cctccgccgc cgcagcagca gtccaagccg ccgtgttcag aagcttgcat
     2761 gaggctgcgt tacacaagct cgcatggggg cagcaaatct caagcaaaca gcgcgattca
     2821 aaatcatcag tctcagtcgc aacatggcgc gagtggatct tggtctgcgg gagccgaagg
     2881 cagttgggga gggaatcaaa atcagggcgc caagtatggg attgcgtttg gcgcgattgg
     2941 tggtggtgtc gcgggaagtt gggtagttgc aggtctgctt gcgccggaga ccggtggcgc
     3001 cagcgtggga gttgaatttc tcaccctttt gttcgagctt ggtgagccaa cagcaacaat
     3061 cgtgggtgca ggaggattgc ctggagctgc ggttggcgct actgccggtg caacagcagg
     3121 taccgtcatt gaccaatcaa acgatgacga taaggataag tagcgtgaat atattccccg
     3181 ccagatgttt caagacaccg atctcgctac tggtatcaac cgtggcggcc tttgaaatct
     3241 gctgtgcgct gccgttctgg gcattcctcg tcattcagtg gtctggaaat gaaacgcttt
     3301 ctgcacacag ggcgctgtat tccggagtag tgattttcgg gacctttttt ttggcagccc
     3361 tgattttttg ggtggccata gcggagccgc ttaggaaaga acgtaatcaa aggcaaaaag
     3421 aaaagtgaaa caaaagcaca aggcccgcaa ttgcgggcct tgttgttttg agcattggct
     3481 gttgcgctac tgatgcgcgg cgagcactga atccagcacc ttcgagatcg cctgctgctg
     3541 cgccttgggt agcgactgca cgcgctcgag ctgctgctgc agctttggcg ccggcccgcg
     3601 cttgccgacg ctacgcctgg ccggcatgcc gatcagttct tcgatggcga cgccgagcac
     3661 acgcgcgacg gtaggcagca tcgcggcggc gatgcgggcg gtgccgcctt cgtagtgggc
     3721 catggtctgc tgggccacgc ccagttcctg ggccaggtcg gtttgcgtca ggccgagcgc
     3781 cttgcggtgt tcggcgatgc ggcgcccgag ccgctggtag aactcctggt ctttcatggc
     3841 gggagcagcc gtgtcgttgg gtcgatcgag catagggcgg ctcctgggtg gcggcggttg
     3901 actataccca tgatatgggt accctgtgta cgggttattt ttacccaaaa ccccttgaca
     3961 ggtttttgcc cggaggcccg cccatgccgc aagcccttga tccttcgtcg gcgttggccg
     4021 cgccgctggc ggcgccgcag gcggccgggg tgcagatcgc gggcgaggac gacatccgcc
     4081 tgcagcgcgg cccgcgcagc tatcgcgtgc gcggcctggc gcgcaacctg tcggccgagg
     4141 cgttgaaggt gacgctgcgg ctgtcggccg gcgagcattt gcacctggac acgctggacc
     4201 tgtaccaggc gcgtgcgcgc agcgcgttcg ccaaggccgc ggcgctcgag ctgggcgtgg
     4261 ccgagtcggt gatcgccggc gacctggcgg cgctggtgat cgcgctggag ccgctgcagg
     4321 aagccgcgat ccgcggcgcg ttgcagccgg acacgtcgcg cgatgcgccg acgctgaccg
     4381 aagcccagga agccgccggc ctggcgctgc tgcgctcgcc cgacctggcc gagcgcatcg
     4441 tggccgacgt cgaggcgatc ggcgtggtcg gggaggccac caatgcgctg gtggggtatc
     4501 tggcgatggt gtcgcgcttg ctggccaagc cgctggcgat cctgatccag tcgacgtcgg
     4561 ccgccggcaa atcgacgctg atggacgcgc tgctgtcgct gctgcccgaa ggccagcgcg
     4621 tgcactacag cgcgatgacc gggcaatcgc tgttctacct gggcgagaag gacctgcagc
     4681 acaaggtgct ggccatcgcc gaggaagaag gcgtgcgcca ggcggcgtac gcactgaagt
     4741 tgctgcagag ccagggcgag ctgaccatcg catcgacggg caaggatcca cagaccggca
     4801 agctggtgac ggccgagtac cgcgtcgaag gcccggtgat gctgtgcctg accacgaccg
     4861 ccatcgacat cgacgaggaa ctgctgaacc ggtgcctggt gctgacgatc aacgagaccg
     4921 ccgagcagac ggcggcgatc cagcagcgcc agcgcagtgc gcgcacgttg gcgggcctgc
     4981 aggcgcaggt acgcagcgat cacgtgctga ccgcgcaccg cgccgcgcag acgctgctgc
     5041 ggccgctggc ggtggtgaac ccgttcgccg aatccctgac gttcgccagc gaccgggtgc
     5101 ggctgcggcg cgatcatgtg aagtacctgg cgctgatcga tgcgatcgcg ctgctgcacc
     5161 agcaccagcg caccgtgcgc accgtcgagg tcgacggcgc cggcgtcgag tacatcgagg
     5221 tcgtgcaatc cgacatcgcg ctggccaacc ggctggcgca tgcggtgctg gggcgttcgc
     5281 tggacgagct gccgccgcag acgcggcgcg tgctggcggc gctggatgcg tgggtggcgg
     5341 cgcaggtggg cgacaccgac acgccgcggg gcgatgtgcg cttcacccgg cgcacagtgc
     5401 gcggtcacct ggggctgagc gacacgcagc tgcgcgtgca cctggagcgg ctggtgacgc
     5461 tggactacgt gctggcccat gccggcaagc aggggcagca gtacgcctac agcctgcgct
     5521 tcgatggcga tgcgggcagc gatgcggcgc agctggccgg gctgtcggtg ttgggtgtgg
     5581 cggagactga ggcatccaaa cccacttcgc ggggtaaaac cgcaacttcg cgggggtcag
     5641 accccgactt cgcggggggt tcgcggggcg ttcgcggtgg ggtcgcggcc ccttcgcggg
     5701 gcgccgggtc gagcaaaaag cccttgaaaa cagccgcgaa cgggcgtgct gccgcacttg
     5761 cccccgaatc cgcacgtcgc cgggcacccg cgcaccgccg tggcaacggc gcgacggcgc
     5821 cctgatggcg ggcaagacca cccagcggat ccagtggctg ctggggccgg tcgcgcccga
     5881 cagcgtggcg gcgtgggtgc ggcgttacct ggaggcgttg gaaacgcagc accgcagccg
     5941 gtcggtgcgc aaggcgtgcc gggcgcggct ggcgcacttc aatgcctggt gcgtggagcg
     6001 cgggatcgag cggcccgaac aggtcacgca cgcgacgatg gcgcggttcc agcgccacct
     6061 gttcgtgtac cgcaaggccg acgggcaacc gctggcgatc aacggccagg tggtgacgct
     6121 gcagaccctg caggcgttct tccgctggct ggtccggcac ggacacttgc cgtggaaccc
     6181 ggcggcggac ctggagttgc cgcggcgcag cgacgagttg cgcgagccgc tgacgctgga
     6241 agaggtcgaa gcggtgctgg ccttgccgga cctggccgat ccgctggggc tgcgcgaccg
     6301 ggcgatgctg gaggtgctgt acgccaccgg catccgccgc gccgagctgc tggtgctgat
     6361 cgtgagcgac atcgaccgca gccgcggcac cttgcacgtg cgcctgggca agggccggcg
     6421 cgaccggttc gtgccggtgg gcgaacgcgc cctggcgtgg atcgcgaagt acgagcgcga
     6481 ggcgcggccg gtgctgctgg ccgacccggc cgaggtgcgg ctgttcgtga actacgccgg
     6541 cgtgccgctg accgcgcaca gcctgacctg gcgggtgcgc aagtacttcc tgcaggcggg
     6601 gatcaccaag cgcggcgcgt gccacctgtt ccggcacacg atggccaccg cgatgctgga
     6661 caacggcgcc gacatccgcc atgtgcagga gatgctgggg caccggcagc tgaccaccac
     6721 gcagcggtac acgcacgtgt cgatcgcgcg gctgaaggcg gtgcatgcgg ccacgcatcc
     6781 ggcggcgaac ctggcgcggc gcgagcggga ggcgctggag gcgccggact gacgcgaagg
     6841 ggtggaagtg aatgaatctt cttcctgatc tgcaggagga aaacgtgtgc ctgaagatct
     6901 aacgcctgaa ttaagccgac tcgtgtagcg gagcaaacaa aatggcaagc tctttctgcc
     6961 attttgtttg tggagcgaaa cgagttcggc ttgaatgaac tgttaggcgc ctcgccaagg
     7021 ggcgaagcgc cgccaaggcc aaacctactg cgatcgggag ctcgttggaa gcccggcgga
     7081 tccgcggcag tgagagccga cggcttggcc tgccgacgcc ttgggcgctg tggccggggc
     7141 gagtagctgg cggggccggg tgtgcggcca ggcgaatggc atccggggcg tggacggccc
     7201 ggggcgaaag gccagggtgc ctacgcgcca acgcgatcta tccgaagccc cgctcttgac
     7261 ctggatgccg cggatgcttg cgatgctgcg accgcacgcg aagccaacac aaagcggttg
     7321 cacaagctgc ctaacgcagg atagccaagc gggcgccaag tggtaccaaa gcgtgttaat
     7381 gcaggatagg gaaaggggcg ccatttgcta gcaggcgggt cggcttaacg gctgcgccgt
     7441 tcaagccgaa cccgcatcgc tgcgccgaac atcccggcga tcggcccagg aagaacgagt
     7501 cggtcagcgc cctgctgcgc agcgcgctgg catattggtc cgggggtcaa tcggcctgcg
     7561 gccgattgaa cataacgctg cgttacgcaa accgccgggc tgtcacggcg cttgcgtggc
     7621 tgcagtgggc cggtgcgtgt ttgcaggcaa gcgccgcgcc agcccggcgc ttcgcgtaac
     7681 aagcgttcat gtcttgcgcc cccggaacag ccagagccga tcaaccaacg gtgcgtgtgg
     7741 ccgcgtgtgc tgcgcacacc cggccacggc atgtgggtgc gccgctgcgc ggcgcgggcg
     7801 ttcggccgca tcgtgggttg cgcgtggaga gccttgcgca ccttcggcca ggccatcggc
     7861 cacgcgcccg tcagcaggga ggaagagggc cggcgccggc gacgacgttg ccctgggcgc
     7921 gatcgcagcg ccttgtgcat cgccaggagc gccaccgtcg gggcgctgcc ccgacacccc
     7981 gtcgccgctt cgatcacttt cctgctgacc tggtggcggc cggcgccgac gatcgatccc
     8041 ggctgaagcc gaaccggcta atcgggacgc gacgagctgc ttgccgatcc ggagtcaggc
     8101 ggcttgacct gcgcgtccgc gcgggtgaaa aaggccatca agggattttt gggggatcgc
     8161 cgaagccgtg tcagggcgtg ttgccaccac gaagccgcgc cgcatcaagg caagccatcg
     8221 ccggcgatgg aaacgtgcgg cggctcaccg ggaataccag cacaccgatg ccctggggac
     8281 gccggtggcg attaccaaca gcagccgcac ggtgacggag cggcgggagt acgagcctta
     8341 cggttaccag acgacgccgg cgttgcagga cgggccgaac tacacggggc atgt
//
LOCUS       DASUYC010000661         2243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1234425, whole genome shotgun sequence.
ACCESSION   DASUYC010000661 DASUYC010000000
VERSION     DASUYC010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234425"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1371)
                     /locus_tag="VF276_10765"
     CDS             complement(<1..1371)
                     /locus_tag="VF276_10765"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX6607383.1"
                     /translation="MSLSLSARHVAKRYGAVVALADASVDIRSGEVLALIGANGSGKS
                     TLSKIINGVVVLDGGQLLLDDRPVRFASPHAAARLGIATVFQELSLVPQMTVAENIFL
                     TREPLRGGGLIDRGAVRRRTEDLLALFAGMFKTPLTPDTPVGPLPPDEKQLIEILKAV
                     SANPRLLILDEATASLDSRQVQRLFELITRWKAEGTAIVFVSHRMEEIFQIADRYTVL
                     RNGQTVGDGLIKDVTTRDLVALMVQGATAENVVGRARSAPADAASRPVLLEVQDLRAD
                     GVGGVSFQLHEGELLGLGGLRGQGQHQLLLALFGDIPHAGRVTVAGSPVQFTHPRQAM
                     TQRLALVPGERAKEGLLLIRSILENLLIPSWRRYGFPLQMARARRDSAGIADRLSLRM
                     AGLDAPVSSLSGGNAQKVVIGKWLLRDPRILMLNDPTKGVDVGTKAEFYKLLAQLCDA
                     GKAVLLY"
     gene            complement(1375..>2243)
                     /locus_tag="VF276_10770"
     CDS             complement(1375..>2243)
                     /locus_tag="VF276_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX6607384.1"
                     /translation="TTDLVIESADTDVPGQIQQLQNLINKGVDAILVNPGDVNGLNDT
                     LQDAIKKGIIVISVDQELPTPNVYNVGIDQKEWAKTSAKWLADKLGGKGNIVEIEGIP
                     GHPANVARMSGVDEVLKNYPDIKVLGKDTGKWDEATGQQVMSNFLAAFPNMDGYWTQD
                     GMAIGAFQAVLAANPAKFPVPVLEARCQALQLWDDRLKVDPNFESIAVANPPGVASTG
                     LRIAVNMLQGKQVNKSKLAGTNGLFFVLPVPVTVTKDNFQQVFQSQCAGKPPAYLLDG
                     IMTDQEVQQFFQ"
BASE COUNT          348 a          746 c          746 g          403 t
ORIGIN      
        1 gtagagcagg acggctttgc ccgcgtcgca gagctgggct agcagcttat agaactcggc
       61 cttggtgccc acatcgacgc ccttggtcgg gtcgttgagc atcagaatgc gcgggtcgcg
      121 cagcagccac ttgccgatca cgaccttctg cgcattgccg ccggataggc tgctgaccgg
      181 cgcatccagc ccggccatgc gcaagctgag ccggtcggcg atgccggcgg agtcgcgccg
      241 cgcccgcgcc atttgcagcg ggaagccgta gcgacgccac gacgggatca gcaagttctc
      301 caggatcgag cggatcagca gcagcccttc cttggcccgt tcgccgggca ccagggccag
      361 ccgttgcgtc atggcctggc gcgggtgggt aaactgcacc gggctgccgg caacggtcac
      421 gcggcccgca tgcgggatgt cgccgaagag ggcgagcaac aactggtgct gcccctggcc
      481 gcgcaggccg cccaggccca gcagttcgcc ttcgtgcaac tggaagctga cgccgccgac
      541 cccgtccgcc cgcaggtcct ggacctccag cagcaccgga cgcgacgccg catccgcggg
      601 cgcggaccgg gcgcgcccga ccacgttctc cgccgtggcg ccctgcacca tcaaggcgac
      661 gagatcgcgg gtggtgacat ccttgatcag gccgtcgccc accgtctggc cgttgcgcag
      721 caccgtatag cggtcggcga tctggaagat ctcttccatg cggtgcgaga caaagacgat
      781 cgccgtgccc tccgccttcc agcgggtgat cagttcaaac agccgctgca cctggcggct
      841 atcgaggctg gccgtcgcct cgtcgaggat tagcagccgg gggttggcgc tgaccgcctt
      901 gaggatctcg atcagttgtt tctcgtccgg ggggaggggg cccaccggcg tatcgggggt
      961 cagcggggtc ttgaacatgc cggcgaacaa cgccaggagg tcctcggtgc gccgccgcac
     1021 ggccccgcga tcgatcaggc ccccgccgcg cagcggttcg cgggtgagga agatgttctc
     1081 cgccaccgtc atctgcggca ccaggctcag ttcctggaac acggtggcga tgcccagccg
     1141 ggcagcggcg tgcggcgagg cgaagcggac aggccggtca tcgaggagta gttgccctcc
     1201 gtcgaggaca actaccccgt tgatgatctt gctgagcgtg ctcttaccgc tgccgttggc
     1261 cccgatcagc gccagcacct cgccggagcg gatgtccaca ctggcatcgg ccagggcgac
     1321 caccgcgccg tagcgcttgg caacatgacg ggcggacagc gataagctca tcgactactg
     1381 gaagaactgc tgaacttcct ggtcggtcat gatcccgtcg agcagatacg ccggcggctt
     1441 gcccgcgcac tggctctgga agacctgctg gaagttgtcc ttggtcacgg tcacgggcac
     1501 gggcaggacg aagaacaggc cgttcgtgcc ggcgagcttg ctcttgttca cctgcttgcc
     1561 ctggagcatg ttcactgcga tgcgcaggcc ggtcgaggcg acgcccggcg ggttcgccac
     1621 ggcgatgctc tcgaaattcg ggtccacctt caggcgatca tcccagagtt gcagcgcctg
     1681 gcagcgggct tcgagcaccg gcaccgggaa cttggccggg ttggcggcca gcacggcctg
     1741 gaaggcgccg atcgccatgc cgtcctgcgt ccagtagccg tccatgttgg ggaaggcggc
     1801 caggaagttg gacatcacct gctggccggt ggcctcgtcc cacttgccgg tgtccttgcc
     1861 gagcaccttg atgtcgggat agttcttgag cacttcatcc accccgctca tgcgggcgac
     1921 gttggccggg tggccgggga tgccctcgat ctcgacgatg ttgcccttgc cgccgagctt
     1981 gtcggccagc catttggccg aggtcttggc ccattccttc tggtcgatgc ccacattata
     2041 tacgttcggc gtcggcagtt cctggtccac cgagatcacg atgatgccct tcttgatcgc
     2101 gtcctgcagc gtgtcgttca gcccgttcac atcgcccgga ttgaccagga tggcgtccac
     2161 gcccttgttg atcaggtttt gcaactgctg gatctggccc ggcacgtcgg tgtcggcgct
     2221 ttcgatcacc aggtcggtgg tca
//
LOCUS       DASUYC010000662         5115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1835933, whole genome shotgun sequence.
ACCESSION   DASUYC010000662 DASUYC010000000
VERSION     DASUYC010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5115
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1835933"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="VF276_10775"
     CDS             complement(<1..826)
                     /locus_tag="VF276_10775"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6607385.1"
                     /translation="MAPPTHLPQPLTSFIGREQEIAAIRRLLAGARLLTLTGTGGSGK
                     TRLALQVAAAVGDDFRDGVLFVPLAALHDPALVIPTIAGALGLTEAGGRPLVESLKQH
                     LRDRQLLLVLDNFEQVSEAAPAVADVLEACPEVRALITSRAALHVYGEQEFAVPPLAL
                     PPEAGAGAALPDSEAVRLFVVRARENSPDFHLTPENAAAVVEICRRLDGLPLAIELAA
                     ARLKILSPAAMLARLADSAAAPTLRLLTGGARNLPARQQTLRDAIAWSYDLLDPAEQ"
     gene            989..2101
                     /gene="menC"
                     /locus_tag="VF276_10780"
     CDS             989..2101
                     /gene="menC"
                     /locus_tag="VF276_10780"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019121610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HEX6607386.1"
                     /translation="MTTIEQITLTHIAMPLVTPFETSFGRDVVRETITVETRAADGAG
                     WGQVVAFEEPSYSYETLDTCWTILTRYLIPLVFARPLTDLEDLQSRWAWVRGHNMAKA
                     ALEGAAAALLSAQAGQSLSAYLGGTRDRVPVGVSVGIQESPEALRDVVAGFIAEGYGR
                     IKLKIKPGKDVAYVAAVREAFPDVALMADANSAYTLADADLLRQLDAFKLIMIEQPLA
                     HDDIYEHSLLQRQLATSICLDESIHSVADARAALELGSCGIINIKVGRVGGLLPARAI
                     QELCRSREVPVWCGGMLETGIGRAANLAIASLPGFTLPGDISASNRYYKHDIAAPEFT
                     LNPDSTITVPTAPGLGVTVDRAALAAVTLHSESFRP"
     gene            2117..3250
                     /locus_tag="VF276_10785"
     CDS             2117..3250
                     /locus_tag="VF276_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX6607387.1"
                     /translation="MQDLLAALTAQTEALVEDLLIWCAVESPSTDPATTTRMAEIVGA
                     YMAARGAQVDVVPGRAHGALVRAGWPGPADAAPILLLGHHDTVHPLGSLAHNLPRVEQ
                     GRCYGPGGYDMKAGLLMAGAALHALIATSRPLPRPVILISTADEEIGSYDSRALIEET
                     ARQAAAVLVLEPAAGSGALKTARKGTGMFTVTTQGRAAHAGVDHAAGVSAIAELAHQI
                     LALTALTDYARGVTVNVGQISGGTAVNTVAAEATAHVDLRVTTWDDARRISDRILGLQ
                     PVLPGAVVTVTGGVDRPPMERPPGTVRLFDLARRLGREIGLDLQETATGGASDGNLTA
                     ALGVPTLDGLGACGDGAHTDHEYVTIADLAPRTTLLASLLLHI"
     gene            3321..4151
                     /locus_tag="VF276_10790"
     CDS             3321..4151
                     /locus_tag="VF276_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607388.1"
                     /translation="MVEVITIRRLANTDEYVAAEQLQQDCWQSGPIEAVPLHMMITLQ
                     KEGGLVLGAFTPEGRLVGFVLGFLGREGAGLKHCSHMAAVHPDYSNHHIAWRLKMAQR
                     EILLAEGLEVCTWTFDPLESRNATLNIAKLGAISRTYKRHIYGQMRDGLNALLPTDRL
                     QVRWELNSARVRLCAETGGRVEAPVAAGALTQVAVRDGVPVLAGTLLGWEGRTVGIQV
                     PREFQPIKRENPDVAMEWRMGTRALFEAAFAGGYTVVNVTPDPDDPALLTRYTLQLRV
                     "
     gene            4193..5017
                     /locus_tag="VF276_10795"
     CDS             4193..5017
                     /locus_tag="VF276_10795"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HEX6607389.1"
                     /translation="MNNRPITSQPAVSIVIPAYNEARRLPATLPRVLAFADSLGRPFE
                     IVVVDDGSTDATRQMVRDMAAARPEVRLIENPHSGKAFTVRTGMLAAAGRYAVQADAD
                     LSTPPAEFARLIAALDAGAHVAIGSRAGRPGAPWYRNVMSFSWRVLVATLGVRGFHDT
                     QCGFKAYRAETAREVFARALLYNAPAQGLKQARVTAASDVELLVIARRLGYTIAEVPL
                     EWNYAENTKISPVRDSLWAFVDLLRISRHVLRGAYGGPARYPAPVIDDVPGGPPAG"
BASE COUNT          783 a         1777 c         1823 g          732 t
ORIGIN      
        1 cctgctctgc cgggtcgagc aggtcgtagc tccaggcgat ggcatcgcgc aaggtttgct
       61 ggcgggctgg caggttgcgc gccccgccgg tgagcaggcg cagggtcggt gcggcggcgg
      121 agtccgccag ccgggccagc atggccgcgg gcgagaggat cttaagccgg gccgcggcca
      181 gttcgatggc aagcggcagc ccatccagcc gccggcagat ctcgactacc gccgccgcgt
      241 tctccggcgt caggtggaag tcggggctgt tctcgcgggc gcggacgaca aacaggcgca
      301 ccgcttcgga gtcgggcagc gcggccccgg ctccggcctc gggcggcagg gccagcggcg
      361 gcacggcgaa ttcttgctcg ccgtagacat gcagcgccgc ccggctggtg atcagggcgc
      421 gcacctccgg acaggcttca agcacatccg cgacggcggg cgcggcctcg ctcacctgct
      481 cgaagttgtc gagcaccagc agcagttggc ggtcacgcaa gtgctgcttg aggctctcga
      541 ccagcgggcg ccccccggct tcggtcagac cgagcgcccc ggcgatggtg gggatgacca
      601 gggccgggtc gtgcagggcg gccagcggca cgaacaggac gccgtcgcgg aagtcatccc
      661 cgactgcggc ggccacttgc agcgccaggc gcgtcttgcc gctgccgccc gtcccggtca
      721 gcgtgagcag ccgggcgccg gcgagcaggc gccggatggc ggcgatctcc tgttcgcgcc
      781 cgatgaaact ggttagcggt tgcggtaaat gggtcggcgg ggccaccggc atcactcctc
      841 ttgatcactc tgattataca cgcgatagcc tccggagact accggtgcgg ttggcagccg
      901 ggcgccgggc ggggtataat tgtcgcaacc aggcccccgt cccatccccc ggacccacgt
      961 agcagggcag gcgccgccag gagacaccat gaccacgata gaacagatta cattgaccca
     1021 tatcgccatg ccgctcgtca cgccgtttga gaccagcttc gggcgcgatg tcgtgcgcga
     1081 gacgatcacc gtcgagacgc gcgcggcgga cggcgccggg tggggccagg tggtggcctt
     1141 cgaggagccg tcttattcct atgagacgct ggatacctgc tggacgatcc tgacccgcta
     1201 cctgatcccg ctggtctttg cccgcccgct gaccgatctg gaggatctgc aaagccgctg
     1261 ggcctgggtg cgtgggcaca acatggcgaa ggcggcgctc gaaggcgcgg cggcggcgct
     1321 gctcagcgcg caggccgggc aatcgctgtc ggcctatctc ggcggcaccc gcgaccgcgt
     1381 gcccgtgggc gtgagcgtgg gcatccagga gtcgccggag gcgctgcgcg acgtggtggc
     1441 cgggttcatc gccgagggct acgggcgcat caagctgaag atcaagccgg gcaaggacgt
     1501 ggcctacgtc gccgccgtgc gcgaagcctt ccccgacgtg gcgctaatgg ccgacgcgaa
     1561 ctcggcctac acactggccg acgccgacct gctgcgccag ctcgacgcct tcaagctgat
     1621 catgatcgag cagccgctgg cccacgacga catttacgag cacagcctgt tgcagcgcca
     1681 actcgccacg tcgatctgcc tggacgagag catccacagc gtggccgacg ctcgcgccgc
     1741 gctcgaactg ggcagttgcg ggatcatcaa catcaaggtg ggccgcgtgg gcggcctgct
     1801 gcccgcgcgg gcgatccagg agttgtgccg cagccgggag gtgccggtct ggtgcggcgg
     1861 catgctggag acgggcatcg gacgcgccgc caaccttgct atcgccagcc tgcccggctt
     1921 caccctgccc ggcgacatct cggccagcaa ccgctactac aagcacgaca tcgccgcgcc
     1981 cgagttcacc ctgaacccgg acagcacgat caccgtgccc acggcgcccg gcctgggcgt
     2041 caccgtggac cgcgccgcgc tggctgccgt gaccctccac agcgagagct ttcgtcccta
     2101 accccaggag gttctgttgc aagacttgct cgcggccctg accgcacaaa ccgaagcgct
     2161 cgtcgaagac ctgctcatct ggtgcgctgt ggagtcgcct tcgaccgacc cggcgacgac
     2221 gacgcgcatg gccgagatcg tgggcgccta catggcggcg cggggggcgc aggtagacgt
     2281 ggtgccgggg cgggcgcatg gcgcgctggt ccgtgccggc tggcccggcc ccgccgacgc
     2341 cgcgccgatc ctgctgctgg gccatcacga cacggtccac cctctcggca gcctggccca
     2401 caacctgccg cgggtcgagc agggccgctg ctacggcccc ggcggctacg acatgaaggc
     2461 gggactgctg atggcgggcg ccgcgctgca cgccctgatc gcgacgagcc ggcccctccc
     2521 ccgcccggtg atcctgatca gcacggcgga cgaggagatc ggcagctacg actcgcgggc
     2581 gctgatcgag gagacggcgc ggcaggccgc cgccgtgctg gtgctggaac ccgccgcggg
     2641 cagcggcgcg ctgaagacgg cgcgcaaggg caccggcatg ttcaccgtga ccacccaggg
     2701 ccgcgccgcc cacgcgggcg tcgatcacgc cgcgggggtg agcgccatcg ccgaactggc
     2761 ccaccagatc ctggccctga ccgcgctcac cgactacgcg cgcggcgtca cggtcaacgt
     2821 cggccagatc agcggcggca cggcggtgaa caccgtggcc gccgaggcga cggcgcacgt
     2881 cgatctgcgc gtcaccacct gggacgacgc ccggcgcatc agcgaccgca tcctgggcct
     2941 gcaaccggtg ttgcccggcg ccgtcgtgac cgtgaccggc ggcgtggacc gcccgccgat
     3001 ggagcgcccg ccgggcacag tgcggctgtt cgatctggcc cgccgcctgg gccgcgagat
     3061 cggcctcgac ttgcaggaga cggccaccgg cggcgccagc gacggcaacc tcacggcggc
     3121 cctcggcgtg cccacgctgg acggcctcgg cgcctgcggc gacggcgccc acaccgacca
     3181 cgagtacgtc accatcgcgg acctggcccc gcgcacgacg ctgctggcga gtttgctgct
     3241 gcatatttag ctgtcagcgg tcagccgtca gctatcagaa tttggcctgt taaagcagct
     3301 tcagcgttat gaaggatctt atggtagaag ttatcacgat tcgccgcctg gcgaacaccg
     3361 acgagtatgt ggcggcggag cagttgcagc aggactgctg gcagtctggg ccgattgagg
     3421 ccgtgccgct gcacatgatg atcaccttgc agaaagaggg cgggctggtg cttggcgcgt
     3481 tcacgccgga agggcggctg gttggcttcg tgctcggctt tctgggacgc gagggggcgg
     3541 gcctcaagca ttgctcgcac atggccgccg tccaccccga ctacagcaac caccacatcg
     3601 cctggcgcct gaagatggcc cagcgcgaga ttttgctggc cgaggggctg gaggtctgca
     3661 cctggacgtt cgacccgctg gagtcgcgca acgccacgct gaacatcgcc aagctgggcg
     3721 caatcagccg gacctataag cgccacatct acggccagat gcgcgacggc ttgaacgccc
     3781 tgctgcccac cgaccggcta caggtgcgct gggagctgaa cagcgcccgt gtgcggctat
     3841 gcgccgagac gggcgggcgg gtcgaggcgc cggtggccgc cggggcgctg acccaggtgg
     3901 cggtgcgcga cggcgtgccg gtgctggcgg gcacgctact gggatgggaa ggccgcaccg
     3961 tgggcatcca ggtcccgcgc gagttccagc cgatcaagcg cgagaatccg gacgtggcga
     4021 tggagtggcg catgggcacg cgcgccctgt tcgaggcggc gtttgccggc ggctacaccg
     4081 tcgtcaacgt cacccccgac cccgacgacc cggcgctgct gacccgctac acgctgcaac
     4141 tccgggtgtg agcgggagaa tgatgccaca ggagcagcaa ggccatgacc cgatgaacaa
     4201 tagaccaatc acgagccaac cggcggtctc gattgtgatc cccgcctaca acgaagcgcg
     4261 gcggctgccc gcgaccctgc cgcgcgtgct ggcctttgcc gatagcctgg gccggccgtt
     4321 cgagattgtg gtcgtggacg acggcagcac ggacgccacc cggcagatgg tgcgggacat
     4381 ggccgccgcg cgcccggagg tgcggctgat cgagaacccg cacagcggca aggcgttcac
     4441 cgtgcggacg gggatgctgg cggcggcggg gcgctacgcc gtgcaggccg atgccgacct
     4501 ctcgacgccg cccgccgagt tcgcccggct gatcgctgcg ctggacgcgg gcgcgcacgt
     4561 cgccatcggc tcgcgggccg ggcggcccgg tgcgccctgg tatcgcaacg tcatgagctt
     4621 tagctggcgg gtgctggtcg ccacgctggg cgtgcgcggc ttccacgata cgcagtgcgg
     4681 cttcaaagcc taccgcgcgg agacggcgcg ggaggtcttc gcgcgggcgc tgctctataa
     4741 cgcgcccgcg caggggctga agcaggcccg cgtcaccgcc gcgtccgacg tggaactgct
     4801 ggtcatcgcg cggcggctcg gctacacgat tgccgaggtg ccgctggagt ggaactacgc
     4861 cgagaacacc aagatcagcc cggtgcgcga cagcctctgg gcgttcgtgg acctgctgcg
     4921 catctcccgg cacgtcctgc gcggcgccta cggcggcccg gcccgctacc ccgccccggt
     4981 catcgacgat gtgcccggcg gcccgccagc cggctagcag gcgtaatatt gcggtgtagg
     5041 ggccggtttg aaaccggccc gccgtccagc cgatgatcag gcgagcacag tccgtttgcg
     5101 gtgtaggggc cggtt
//
LOCUS       DASUYC010000663         3502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1930585, whole genome shotgun sequence.
ACCESSION   DASUYC010000663 DASUYC010000000
VERSION     DASUYC010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1930585"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2302
                     /locus_tag="VF276_10800"
     CDS             <1..2302
                     /locus_tag="VF276_10800"
                     /inference="COORDINATES: protein motif:HMM:NF042648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="HEX6607390.1"
                     /translation="PYEAGWGTGEFFYWDIDMIRWLEASGYDVAYISDVDTATNPALL
                     QNHRVFLTTGHPEYWTWAEHDNVDALRDSGTNLIFASANEAYWNIRLSASSVGANRVI
                     TCYKDLNLDPIKTSPNATVRFHDPPLNRNENALLGYYFFDDNWYRTEGYNAAWVANAP
                     ANRWYFDCTNIQPGDRINNIVGYEWNTAFDNGSSPPGLDLVSNSPIINYLGRTNPANT
                     VMYTAPSGAYVFSMGTIRWSWGLIDHSYSNNQFQDALLSNDADHRIEQLTANVLDYFA
                     GYWDGQSRSCSGQGFYDIGVRPTRTPMPQPPTATGPTPGPTNTATFTRTPVAAGTATP
                     TRTATMTRTPGPTGTSTATPLPPSATPTPVDTATPTVTSTATDTPTGTATRTATSTRT
                     PTVTRTATVTRTATRTPTSTRTATAIPSATLTATPVPTGTPTRTTSPTATPPDTSTAT
                     PSPTRTATHTATPSATPTDTHTATPTQTATTTPVSSATPTGTRTATASPTHTATRTAV
                     PSLTPAPSSTSTATRTAVAPGGTATRTPVWTATNTPLPTATATVVPCAITFVDVPAGQ
                     TFAVYIHWLACRGIISGYSCGAPGESCPGLYFRPDADVTRGQLLKMVVNSAGWPLLNP
                     ATPAFADVPPGNAFYSFIETGVSHGIISGYDCGGPGEPCNTGRRPYFRPAAAITRGQL
                     SKVIALARGYAIPNPATPTFTDVPASNSFYGYVEAVAAHHIVSGYDCGSPGEPCDGAH
                     RPYFRPGNTATRGQVSKLITLAYGGP"
     gene            complement(2372..3430)
                     /locus_tag="VF276_10805"
     CDS             complement(2372..3430)
                     /locus_tag="VF276_10805"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6607391.1"
                     /translation="MTTRFWRLPAALAAAVLLAGSLLTGPATQAAAPPPDLGAGRVAR
                     NLTYCTGGGQPLTMDLYFPREAAAIPMPVVVFVHGGGWEIGDKDTPDEIAPEMIGRGY
                     AVAAINYRLAPAWRWPAPIEDVKCAIRYLRAEAGTYHLDPDRIGVWGSSAGGHLAVLL
                     GLTDSRAGFDGQGGYADQSSRVQAVVDLYGPTDLPALAQTGSDRMLETLQRVFGAGGK
                     LPAALLRQASPVTYVSGAAPPFLILQGDKDTMVPASQSVELYRRLQAAGVPATLVMVR
                     NGDHGFVPAGGPVSPSQAERTRLIGDFFDRYLLAPTPAHSTAATGLPPVWALPDLAQT
                     IRHQIADLANAYRGMTAR"
BASE COUNT          588 a         1394 c         1053 g          467 t
ORIGIN      
        1 gccctacgaa gcgggctggg gcaccggcga gttcttctat tgggacatcg acatgatccg
       61 ctggctggaa gcctccggct acgacgtggc ctatatcagc gatgtcgata ccgccaccaa
      121 cccggcgctg ctgcaaaacc accgcgtttt cctcaccacc gggcaccccg aatactggac
      181 ctgggccgaa cacgacaacg tcgacgccct gcgcgacagc ggcaccaacc tgatctttgc
      241 ctcggccaac gaagcctact ggaacatccg cctcagcgcc tcctcggtcg gcgccaaccg
      301 ggtgatcacc tgctacaagg atctgaacct cgacccgatc aaaacgtcgc cgaacgcgac
      361 cgtgcgcttc cacgacccgc cgttgaaccg caacgagaat gccctgctcg gctactactt
      421 cttcgacgac aactggtacc gcaccgaggg ctataatgcc gcctgggtgg cgaacgcgcc
      481 cgccaaccgc tggtacttcg actgcaccaa tatccagccg ggcgaccgga tcaacaacat
      541 cgtcggctac gagtggaaca cagcattcga caacggctcg tccccgccgg ggctcgacct
      601 cgtctctaac agcccgatca tcaactacct gggccggacc aacccggcga acaccgtcat
      661 gtataccgcc cccagcggcg cctatgtatt ctcgatgggc accatccgct ggtcctgggg
      721 cctgatcgac cattcgtaca gcaacaacca gttccaggat gccctgctct ctaacgatgc
      781 cgaccatcgc atcgagcaac tgacggccaa cgtgctcgat tacttcgccg gatactggga
      841 cggccagtcc cggtcgtgca gtggccaggg cttctacgac atcggcgtgc ggcccacgcg
      901 cacgccgatg ccgcagccgc ccaccgccac cgggccgacg ccagggccga ccaacacggc
      961 caccttcacc cgcacccccg tcgccgcggg caccgccacg cccacccgca cggcgaccat
     1021 gacgcgcacc cccggcccga ctgggacgag caccgccacg ccgctgccgc ccagcgccac
     1081 gccaacaccg gtagacaccg ccacacccac ggtgacctcc accgcaacgg acacccccac
     1141 aggcacggcc acgcgcaccg ctacttccac gcgcacgccg accgttaccc gcaccgccac
     1201 ggtcacgcgc acggccacgc gcacgccgac cagcacccgc acggccacgg cgatcccctc
     1261 ggcgacgctg accgccacgc cggtgcccac cggcacgccc acccgcacta cgtcgccaac
     1321 cgccaccccg cccgatacca gcaccgcgac cccgtcgccg acccgcacgg ccacacacac
     1381 cgctacgccc tcggcgaccc cgaccgatac gcataccgcc acgcccacgc agacggctac
     1441 gaccacacct gtatcgtcgg cgacgccgac cggcacccgc acggcgacgg catcgcccac
     1501 gcatacggcg acccgcaccg ctgtcccgtc gctaactccg gcgccgagca gcacgtctac
     1561 ggcgacccgc accgcggtcg cgccgggcgg caccgccacg cgcaccccgg tgtggacggc
     1621 gacgaacacg ccgctgccca ccgccacggc cacggtggtc ccctgcgcga tcaccttcgt
     1681 cgacgtgccc gcgggccaga ctttcgccgt ctacatccac tggctggcct gccgcggcat
     1741 catcagcggc tactcctgtg gcgcgccggg cgagtcctgc cccggcctct acttccgccc
     1801 ggacgccgat gtcacgcggg gccagttgct gaaaatggtc gtgaactccg cgggctggcc
     1861 gctactcaac cccgccacgc cggcattcgc cgatgtcccg ccgggcaacg ccttctacag
     1921 cttcatcgaa acgggcgtga gtcacgggat catcagcggc tacgactgcg gcggccccgg
     1981 cgagccgtgt aacaccgggc gccggcccta cttccgcccg gcagccgcaa tcacgcgcgg
     2041 ccagctcagc aaggtcatcg cgctggcccg cggctacgcc atcccgaacc cggccacgcc
     2101 caccttcacc gacgtgccgg cgagcaactc cttctatggg tatgtggaag ccgtcgccgc
     2161 ccaccacatc gtcagcggct acgactgcgg cagccccggc gagccgtgtg acggtgcgca
     2221 tcggccctac ttccgccccg gcaataccgc cacccgcggc caggtctcca agctcatcac
     2281 cctggcctac ggcgggccat aacctcgcgc gggcacacga aaacccccgg tggccggaca
     2341 ggtcatcggg ggtctttgtt cggggcaggt gttaccgtgc cgtcatgccg cgatatgcgt
     2401 tcgccagatc cgcgatctgg tggcggatcg tctgcgccag gtccggcaag gcccagaccg
     2461 ggggcagacc ggtcgcggcg gtgctatgcg ccggggtggg cgccagcagg tagcgatcaa
     2521 agaaatcgcc gatgaggcgg gtccgctcgg cctgcgacgg gctcaccggt ccgcccgccg
     2581 gcacaaagcc gtggtcgccg ttgcgcacca tcaccagcgt cgcgggcacg cccgccgctt
     2641 gcagccgccg gtacagttcc actgattggc tcgccggcac catggtgtct ttgtcgccct
     2701 gcaagatcag gaagggcggc gcggcgccgg agacataagt gaccgggctg gcctgccgca
     2761 agagagcggc aggcagtttg ccgcccgcgc caaagacccg ttgtagcgtc tccagcatgc
     2821 ggtcgctgcc cgtctgggcc agggcgggca gatcggtcgg gccatagaga tcgaccaccg
     2881 cctgcacgcg gctcgactgg tccgcgtagc cgccctggcc gtcaaacccg gcccggctat
     2941 cagtgaggcc cagcagcacc gccaggtgcc cgcccgcgct gctgccccac acgccgatgc
     3001 ggtcggggtc caggtgatag gtgcccgcct cggcgcgcag gtagcggatg gcgcacttca
     3061 cgtcctcgat gggcgcgggc cagcgccacg cgggcgccag ccggtaattg atcgccgcca
     3121 ccgcatagcc gcgcccgatc atctccggcg cgatctcgtc gggcgtgtcc ttatcgccga
     3181 tctcccagcc gccgccatgc acgaacacca ccaccggcat ggggatggcc gcggcttcgc
     3241 ggggaaagta gaggtccatg gtgagcggtt gcccgccgcc cgtgcagtag gtcaggttgc
     3301 gggccacgcg cccggccccc aggtcgggcg gtggggcggc ggcctgcgtg gccggcccgg
     3361 tgagcaggct gcccgccagc agcaccgccg ccgccagcgc cgccggcagc cgccagaatc
     3421 gcgtggtcat tggaatgccc ccctctctcc gccccggccc gatcgcccct gcccaaaggt
     3481 ggcgagggtc ccgtgtgctg ga
//
LOCUS       DASUYC010000664         7022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1891085, whole genome shotgun sequence.
ACCESSION   DASUYC010000664 DASUYC010000000
VERSION     DASUYC010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 7022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7022
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1891085"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..683)
                     /locus_tag="VF276_10810"
     CDS             complement(162..683)
                     /locus_tag="VF276_10810"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6607392.1"
                     /translation="MAAAPDFIVREATPADAAAVIAAAQAVFNEPGVMVLTSPGEFHP
                     TLAEEEQLLADRQAAGNSVFLIAVAGAEVVGMLGCTGGRRQGNRHTALLGMSVRREWR
                     DRGVGSALMARAVEWARGTGIVTRIELDVYVENARARHLYEKFGFEVEGRRRRAIYRD
                     GQYHDDLIMALLL"
     gene            complement(771..2966)
                     /locus_tag="VF276_10815"
     CDS             complement(771..2966)
                     /locus_tag="VF276_10815"
                     /inference="COORDINATES: protein motif:HMM:NF024905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEX6607393.1"
                     /translation="MPAWRKLWQDVVRVDRSQVVFWTALRNAVGVFIPLAIGAVTGQL
                     LLGLTAGTGALQVAFADRPGPYRLRAGRMLLACLAGALSVFVGAATGTIDWLAVVLVA
                     LWGLAAGLLVALGQAATQIGLTGIIVLLVYGAHPLAPGLAAGQALLFLAGGLLQTLLS
                     VAVWPVQRYRPEREALAAVFRKFAAYVEAPSGRDTAPPVTAEITTAATTLLGLGNDHG
                     ATAEALRALLDEAERIRLELLVLNGARKHLHATGTDLSAREALDTIWDAAGRVLTALA
                     GMVATDRPLESDPDALRRIETTLADLRQAPPAETGDQEGLRRAGILSHTAALAGQLHA
                     AVEIVQEGSAAGETGTLRGAATRPVALRPRGALAILRANLNLDSAAFRHAIRLAVCLA
                     VAAALARGLALPRGYWLPMTAALTLRPDYTTTITKGIGRMAGTMLGLVLATGLVHQVF
                     GDLPAQVLLAGVLVFFARSLGLVNFGLSIIPVTGLVVLLTGFAGANPQATILERGLYT
                     LAGGLLGLAAYRLWPTWERTQTPAILADLLDSYRRYFTVVMAGYLHPDQVDSPRVHAA
                     RLAARLARSNAEASVDRLRDEAATSPRDLDFATGLLANSHRFAYAVMAIEAELNHRPE
                     APRPTALQTFVADVDTTLAAIAAALRDSGELPAGLPDLRSDQSALGAAAGTQAESSYQ
                     IAVLTAQTERITNSLNTILHLLRYPATEAASKEAPAPAQPAPQPKGHPG"
     gene            complement(3246..4052)
                     /locus_tag="VF276_10820"
     CDS             complement(3246..4052)
                     /locus_tag="VF276_10820"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX6607394.1"
                     /translation="MHETGEAYVIGDVHGQYRKLLGLLRDAGLIDGNQAWTGGPASLW
                     FLGDFCDRGPDGIGVVDLVMRLQAEAAEAGGQVGALLGNHEVLILAAAHFGTPRTGQG
                     AVFMFEWQRNGGQTEDLARLTPHHIDWISNLPAMALVADRLLIHADALLYLDYGATID
                     EVNAAFRAVLASRDMDAYDRFLGEFSARRAFAENQRTGAATALAFLQRFGGSQLIHGH
                     TPISAMTRQPPAEVTAPLVYAGGRCVNVDAGLFLGSPGFVYHLPPLDRIT"
     gene            complement(4129..5646)
                     /locus_tag="VF276_10825"
     CDS             complement(4129..5646)
                     /locus_tag="VF276_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX6607395.1"
                     /translation="MDPLIIVVAMVFVVLALIVMFTTLFRKVGPNEALIVYGFGLHEP
                     RVVKGGGTLVLPMVQSARSLSLELMSFDVAPEQDLYTRQGVAVTVEAVAQIKVKSDPD
                     SIRTASEQFLNKTPQERENLIRLVMEGHLRGIVGQLSVEEIVKQPEMVSDKMRATSAE
                     DMAKMGLEIISFTIKDVRDKNEYIVNMSKPENAAIRRAAAIAEAEASRDIAMRQATTM
                     REASVAKAQADQERVIAETASATKQAEAQRNLEVARAEYAATVQKQKATADKAYDIEA
                     NVQQQQVIAAQVGVERVQREEQIKVQEAEILRRERELIATVLKQAEIERKRVETLAEA
                     ERQRRILEAQGAAEAVRVQGGGQADANRLQGLADADVIRARGQAEADAMNLRAQAFKQ
                     YNEAAILDKLLSSMPEVVANISKPLSQVDRITIVSTGDGSNGMGAHQLTGDVTRIIAQ
                     FPALVETLTGVKLSDLMSRVAGIDPQAGQTNGHSAESGIIEGKAVVAPRTPGANN"
     gene            complement(5724..6494)
                     /locus_tag="VF276_10830"
     CDS             complement(5724..6494)
                     /locus_tag="VF276_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607396.1"
                     /translation="MLFGSDWLANVYLGFFLFGFLFTSLSLILNLGHFGGGMHDMSHD
                     LGHAGHLPHDLGHAGHGPDFHAGDHAHADAGHGHSLSEQIDGLSPLNLPTILSFLTWF
                     GGAGYIFQSTLHLGPIVSTVLALISGAAGGALVFFILSYVIWRGQTTPMRRADFYLPG
                     TRARVVSGITAEGTGEIVFSKGGSRRVEGARSATGQPLPKGTEVVITRYEKGLAYVQP
                     APVTADIPPPVAARVEPAEVNTHPLNGNGSYTRDLRNQ"
     gene            6775..>7022
                     /locus_tag="VF276_10835"
     CDS             6775..>7022
                     /locus_tag="VF276_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014351342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEX6607397.1"
                     /translation="MAEPGPIYTIGHSTLPRDEFIAVLRGYGIGLLADIRTVPRSRTN
                     PQYNQDVLPTALAEASIGYTHLARLGGLRGRRKDLPAG"
BASE COUNT         1086 a         2234 c         2569 g         1133 t
ORIGIN      
        1 gacagcctgg ttctcgatct cgtagacctc gggatcgctc tggatattgg gcgccggatg
       61 cacgcccggt aaggcgtcgc ctggttcggt catcggttat cgtctccatg ggtgggcgtg
      121 ccggtttcaa accggcccct acagggtacg ggcgaaccgg atcagagcag cagggccatg
      181 atcaggtcgt cgtggtactg gccgtcgcgg tagatggcgc ggcggcggcg gccctcgacc
      241 tcgaagccga acttctcgta gaggtggcgg gcgcgggcgt tctcgacgta gacatcgagc
      301 tcgatgcggg tcacgatgcc ggtgccgcgc gcccactcga cggcgcgggc catcagtgcg
      361 ctgcccacgc cccggtcgcg ccactcgcgg cgcaccgaca tgccgagcag ggccgtgtgg
      421 cggttgccct ggcggcgacc tccggtgcag cccaacatgc cgaccacttc ggcccccgcg
      481 acggcgatga ggaagacgct gttgccggcg gcctggcgat cggccagcaa ttgttcttct
      541 tcggccaggg tggggtggaa ctcgccgggg ctggtgagca ccatcacgcc gggttcgttg
      601 aacacggcct gggcggcggc gatcacggcg gcggcatcgg cgggggtggc ttcgcggacg
      661 atgaaatcgg gggcggcggc catagcggtc actccttcgg cggcagtggg cgggggcgca
      721 aatcgaacgc cgctattata gcagaccgcg gcgcgccggg atagggcagg ctaaccgggg
      781 tgacccttgg gctgcggcgc gggctgcgcg ggcgccggtg cctctttaga ggcagcctcg
      841 gtcgccggat agcgcagcag gtggaggatg gtgttgaggc tgttggtgat gcgctcggtc
      901 tgggccgtga gcacggctat ctggtagctg gattccgcct gcgtcccagc ggcggcgccg
      961 agagcgctct gatcggagcg caggtcgggc aggccggcgg gtagctcgcc ggagtcgcgc
     1021 agggccgccg cgatggcggc cagggtggta tcgacatcgg cgacgaaggt ttgcagcgcg
     1081 gtggggcgcg gcgcctcggg ccggtgattt agctcggctt cgatagccat gacggcatag
     1141 gcaaagcggt gcgagttggc gagcagaccg gtggcaaagt ccagatcgcg cggggaggtg
     1201 gcggcttcgt cccgcaggcg atcgaccgag gcttcggcgt tggagcgggc cagccgcgcc
     1261 gcgagccggg cggcgtggac gcgcggtgag tccacctggt cgggatgcag gtagccggcc
     1321 ataaccacgg taaagtagcg gcggtagctg tccagcaggt cggccaggat ggccggcgtc
     1381 tgggtgcgtt cccaggtggg ccagaggcgg taggcggcca ggcccaggag gccgcccgcc
     1441 agggtataca gcccgcgctc caggatcgtc gcctggggat tggcgcccgc gaacccggtg
     1501 agcagcacga ccaggcccgt gaccgggatg atggacaggc cgaagttgac cagacccagg
     1561 ctgcgggcga aaaacaccag cacgccggcc agcaggacct gggccggtag gtcgccgaac
     1621 acctggtgca ccaaccctgt cgccagcacc aggcccagca tcgtcccggc catacggcca
     1681 atgcccttgg tgatcgtcgt ggtgtagtcg ggccgcaggg tcagggccgc ggtcatcggc
     1741 agccagtagc cgcggggcag ggccagcccg cgggccagcg ccgcagccac ggccaggcag
     1801 acggccagcc ggatagcgtg gcggaacgcg gcggagtcga ggttgagatt ggcgcgcagg
     1861 atggcgagcg cgccgcgcgg gcgcagggcc acaggccggg tggcggcccc gcgcagggtg
     1921 cccgtttcgc ctgcggcgct gccttcctgt acaatctcca cggcggcgtg taactggccg
     1981 gccagcgcgg cggtatggct caagatgccg gcgcggcgca gcccctcctg gtcgccggtc
     2041 tccgccggcg gggcctggcg cagatcggcg agggtggttt cgatgcgccg caaggcgtcc
     2101 gggtcgctct ccaggggccg gtccgtggcg accatcccgg cgagggcggt tagcacgcgc
     2161 ccggcggcgt cccagatggt atccagggcc tcgcgggcag acaggtccgt cccggtggca
     2221 tgcagatgct tgcgcgcgcc gttgagcacc aggagttcga ggcggatgcg ttcggcctcg
     2281 tcgagcaggg cgcgcaaggc ttcggcggtg gcgccgtggt cgttgcccag ccccagcagg
     2341 gtggtggccg ccgtggtgat ctcggcggtc acgggcggcg cggtgtcgcg cccggagggc
     2401 gcctcgacat aggcggcgaa cttgcggaac acggcggcga gggcttcgcg ctccgggcga
     2461 tagcgctgga cgggccagac ggccaccgag agcagggttt gcagcaggcc gcccgccagg
     2521 aaaagcagcg cctgcccggc ggcgaggccc ggcgccaggg gatgcgcgcc gtagaccagc
     2581 agcacgatga tgccggtgag gccgatctga gtggcggcct gccccagggc caccagcagg
     2641 ccggccgcca ggccccagag cgcgaccagc acgacggcca gccaatcgat cgtgccggtg
     2701 gccgcgccca caaagacgga aagcgccccg gcgagacacg ccagcagcat gcggcccgcg
     2761 cgcagccggt aggggccggg ccggtcggcg aaggcgacct ggagggcgcc cgtgcccgcg
     2821 gtcaggccga gcagcagttg gccggtgacc gccccgatcg ccagcgggat gaacacgccg
     2881 acggcgttgc gcagcgccgt ccagaacacg acctgcgagc ggtcgacacg cacaacgtct
     2941 tgccagagct tccgccaggc gggcaccgtc tccatagcga ccgtattgca ctaagcgtgc
     3001 ccgttgtgtg gagttgcaag ccgggcgccg taatctgtca caaggtgttt gtcactctga
     3061 gcgcagcgaa gagtctgtcc tgtgggccag gagacgagat tcttcgctgc gctcagaatg
     3121 acaagggcga ggcgacggct ggcggggcat tgtcactcgg agcgcagcga agagtctcgt
     3181 cctgtgagct aagagacgag attcttcgct gcgctcagaa tgacaagatc aggatgacga
     3241 actactcagg tgatccggtc caggggcggc aggtggtaga cgaagccggg gctgccgagg
     3301 aacagcccgg catcgacgtt gacgcagcgc ccgccggcat agaccagcgg cgccgtgacc
     3361 tcggcaggcg gctggcgagt catcgcgctg atcggcgtgt ggccgtggat caactgcgac
     3421 ccgccgaagc gctgcaagaa cgccagcgcg gtggccgcgc cggtacgctg gttctcggca
     3481 aaggcgcgcc gggcggagaa ttcgcccagg aagcggtcgt aggcgtccat atcccggctc
     3541 gccaggaccg cgcggaaggc ggcgttcacc tcgtcgatgg tggcgccgta gtccaggtag
     3601 agcagggcgt cggcgtggat aagcaaccgg tcggccacaa gcgccatcgc cggcaagttg
     3661 gagatccagt cgatatggtg cggggtgagc cgggccaggt cttcggtctg cccgccgttg
     3721 cgctgccact caaacatgaa gacggcgccc tggccggtgc gcggggtgcc gaagtgcgcc
     3781 gccgccagga tcagcacctc gtggttgccg agcagcgcac ctacctgccc gccggcctcc
     3841 gcggcctcgg cttgcaggcg catcaccagg tccaccacgc cgatgccgtc ggggccgcgg
     3901 tcgcagaaat cgcccagaaa ccagagcgag gccgggccgc cagtccaggc ctggttgccg
     3961 tcgatcaggc cggcgtcgcg cagcaggccc agcagcttgc ggtactggcc gtgaacatcc
     4021 ccgatgacat acgcttcgcc ggtctcgtgc atggtcttcc tctgcggcaa aacaggacgg
     4081 gcgcactccg cgagtgcgcc cgtcggcgtg attagcgcct atgcggtgtt agttgttggc
     4141 gcccggcgtg cgcggggcca cgaccgcctt gccttcgatg atgccggact cggcgctatg
     4201 gccgttggtc tgcccggcct gcgggtcgat gcccgccacg cggctcatca ggtcgctcag
     4261 cttgacgccg gtaagtgttt cgaccagcgc cgggaactgg gcgatgatgc gggtcacgtc
     4321 gccggtcaac tggtgggcgc ccatgccgtt gctgccgtcg ccggtcgaga cgatggtgat
     4381 gcggtcgacc tggctgagcg gcttgctgat gttggcgacc acttcgggca tgctgctgag
     4441 caacttgtcg aggatggcgg cctcgttgta ctgcttgaag gcctgggcgc gcaggttcat
     4501 ggcgtcggct tcggcctgcc cgcgggcgcg gatcacgtcg gcatcggcga gaccctggag
     4561 gcggttggca tcggcctggc cgccgccctg gacgcgcacc gcttcggcgg caccctgggc
     4621 ttcgaggatg cgccgctggc gctcggcttc ggccagcgtc tcgacgcgct tgcgctcgat
     4681 ctcggcctgc ttgagcacgg tggcgatcag ttcgcgctca cggcgcagga tttcagcctc
     4741 ctgcaccttg atctgctcct cgcgttgcac gcgctcgacg ccgacctggg cggcgatcac
     4801 ctgctgctgc tgcacgttgg cttcgatgtc atatgccttg tcggcggtgg ccttctgctt
     4861 ctgcacggtg gcggcgtatt cggcgcgggc cacttcgagg ttgcgctggg cctcggcctg
     4921 cttggtcgcc gacgccgtct cggcgatgac gcgctcctga tcggcctgcg ccttggcaac
     4981 cgacgcctcg cgcatggtcg tcgcctgccg catggcgatg tcgcggctgg cctcggcctc
     5041 ggcgatggca gcggcgcggc ggatcgcggc gttctcgggc ttgctcatgt tgacgatgta
     5101 ttcgttcttg tcgcgcacgt ccttgatggt gaaggagatg atctccaggc ccatcttcgc
     5161 catgtcttcc gccgaggtgg cgcgcatttt atcggacacc atctccggct gcttgacgat
     5221 ctcctcgacc gacaactggc cgacgatgcc gcgcaggtgg ccttccatca ccagccggat
     5281 caggttctcg cgctcctgcg gggtcttgtt caggaattgc tcagacgcgg tgcggatgct
     5341 gtcggggtcg ctcttgacct tgatctgggc gacggcctcg acggtcacgg ccacaccctg
     5401 gcgagtgtag agatcctgct ccggggccac gtcgaatgac atcagttcga gcgagaggct
     5461 gcgggcgctc tgcaccatgg gcaagacgag cgtgccgccg cccttcacca cgcgcggctc
     5521 atgcagcccg aagccgtaga cgatcagggc ctcgttgggt ccgactttgc ggaacagggt
     5581 cgtgaacatc acgatcagcg ccaggaccac gaacaccatt gcgaccacta tgatcagggg
     5641 gtccatgtta acctatcctc tccttcatac cacgtcgctc aaatcagacg tggtagatgt
     5701 aaattgccaa ctcgcaggca acgttactgg ttgcgtaaat cgcgggtata agatccatta
     5761 ccattcaacg ggtgcgtgtt gacctcggcg ggttcgaccc gcgcggcgac gggcggcggg
     5821 atatccgccg tgaccggggc cggttggacg tacgccaacc ctttctcgta gcgggtgatg
     5881 accacctcgg tgcccttggg caagggctgg ccggtggcgc tgcgcgcgcc ctcgacccgg
     5941 cggctgccgc ccttcgagaa gacgatctcg ccggtgccct cggcggtgat gccgctgacg
     6001 acgcgggcgc gcgtgcccgg cagatagaaa tcggcccggc gcatgggtgt tgtctggccc
     6061 cgccagatca catagctcag gataaagaaa acgagcgcgc cgcccgcggc cccgctgatc
     6121 agcgccagca cggtgctgac gatggggccg agatgcaggg tgctctggaa gatgtagccc
     6181 gcgccgccga accaggtcag gaacgacagg atggtcggca ggttcagcgg actcaggccg
     6241 tcgatctgct cgctgaggct gtggccgtgg ccggcgtcgg cgtgcgcgtg gtcgccggca
     6301 tggaaatcgg gcccgtgccc ggcgtggccc aggtcatggg gcagatgccc ggcgtgtccc
     6361 aggtcgtgcg acatatcatg catgccgccg ccgaaatggc ccaggttcaa gatcagcgac
     6421 agcgacgtga acaggaagcc aaagaggaag aaacccaggt agacattggc tagccaatcc
     6481 gagccgaaga gcatcaggta ctcctttcca tcgcccagac tgccaaccgg gcgtgcgcct
     6541 tcagatgggg aagggaggcc gtcgggttgt ccaagcgccc gctggatggg gttccggacg
     6601 ctttgtcata gtatacggcg aaaccggggc cagggttaca cactttcgtc tcgccatgat
     6661 tatagcatac tctttcaggg gaggccaatc aagcgggcgt gcgctacggg ggacggcggc
     6721 gttgcatata tcgttatgcg cgcgtagagc aggttggtgt gggagtgaac gcgggtggcg
     6781 gaaccggggc caatttatac catcgggcat tcgaccttgc cgcgggacga gtttatcgcc
     6841 gtgctgcgcg gctacggcat cggcctgctg gccgatattc ggacggtgcc gcgctcgcgg
     6901 accaatcccc agtataacca ggacgtgctg cccaccgcgc tggccgaggc gagtatcggc
     6961 tatacgcatc tggcgcggct gggcgggctg cgcgggcggc gcaaggatct gcccgcgggc
     7021 ga
//
LOCUS       DASUYC010000665         2001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1963104, whole genome shotgun sequence.
ACCESSION   DASUYC010000665 DASUYC010000000
VERSION     DASUYC010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1963104"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..861
                     /gene="plsY"
                     /locus_tag="VF276_10840"
     CDS             205..861
                     /gene="plsY"
                     /locus_tag="VF276_10840"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEX6607398.1"
                     /translation="MDLWVLAILILAAYLIGALPSGMVIGALHGVDLRQHGSGKTGAT
                     NVSRVIGRRAALLVFVLDALKGALPVLIARLIDWHDPNYEAIAIGCAAVAALIGHIWS
                     IWIRVFTGAWGGGRGVATAIGTMVAVNPWVALFGLIIGVPIIAISRYVSLGSILGVTA
                     GSLLMVLLVFLQRLSPWLAVYALAAGGLVVVLHHDNIERLLKGTERKFSLKPGQPRQH
                     "
     gene            962..1456
                     /locus_tag="VF276_10845"
     CDS             962..1456
                     /locus_tag="VF276_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014901673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HEX6607399.1"
                     /translation="MWTLGILTLSDRSAAGQRDDVSGPTARDLLLAALPGAEVRRMAV
                     IPDDRPTIETTLRAWADEDRLALVVTTGGTGLAPRDVTPDATLAVLDYQVPGLAEAMR
                     AASLAHTPLAMLSRAVAGVRGRTLIVNLPGSPKAVRECLAAIAPALPHGLEKLAGDPN
                     ECAP"
     gene            1559..>2001
                     /locus_tag="VF276_10850"
     CDS             1559..>2001
                     /locus_tag="VF276_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEX6607400.1"
                     /translation="MTDKISRQMARNVMLAAQGLDQLPRQPAGKADVLEAVRRMYLLQ
                     IDTIHVVARSPYLVLWSRLGDYQPVWLDQLLAEGALFEYWAHAMCFIPIEDYPLYRRR
                     MLDAVRQEVWPVKWAVKWAQENPQVMERVRTHLRENGAVRSAEFE"
BASE COUNT          300 a          688 c          691 g          322 t
ORIGIN      
        1 cccccggccc ctccccgccg cggggagggg agatcgcgcc ctgggccgat acgcgctatc
       61 gtcgcccccc ttccagcggc gggaaggggg ggtggggggt taggttggct ggacaccccg
      121 atcaggctat tgccgcgcgc ccgcggcgga tgtatactta tgccacccgg cgcatcgcct
      181 ttgacaatcg aaaggagcgg cacgatggac ttgtgggtac tcgccatcct gattcttgca
      241 gcatacctca ttggcgccct gccgagcggg atggtcatcg gcgccctcca cggggtcgat
      301 ttgcggcagc acggcagcgg caagacgggc gcgacgaacg tgagccgggt catcggccgg
      361 cgcgcggccc tgctggtgtt tgtcctcgac gcgctcaaag gcgccctccc tgtgctgatt
      421 gcccggctca tcgactggca cgaccccaat tacgaggcca tcgccatcgg ctgcgcggcg
      481 gtggcggcgc tcatcgggca catctggtcg atctggatcc gcgtgttcac cggcgcctgg
      541 ggcggcgggc gcggcgtcgc caccgccatc ggcaccatgg tcgcggtcaa cccctgggtg
      601 gccctgttcg gcctgatcat cggcgtcccg atcatcgcca tctcgcgcta tgtttcgctt
      661 ggctcgatcc tcggcgtcac ggcgggcagc ctgcttatgg tgttgctggt gttcctgcaa
      721 cggctgtcgc cctggctggc cgtctacgcg ctggccgccg ggggcctcgt ggtcgtgctg
      781 caccacgaca acatcgagcg gctgctgaaa ggcaccgagc gcaagttcag cctcaagccc
      841 ggccagcccc gccagcacta ggctgctatc ccggcggggg cattgtagca cagcgcgccg
      901 cccgcacgca aggcagccaa cgtgaacttg tgcatcgaag gaagatgagg gatacgggcg
      961 tatgtggacc ctgggcatct tgacgctgag cgaccgcagc gccgcgggcc agcgcgacga
     1021 cgtgagcggc cccacggcgc gcgacctgct gctggcggcg ctgcccggcg ccgaggtgcg
     1081 gcgcatggcc gtcatccccg acgaccgccc caccattgag accaccttgc gcgcctgggc
     1141 cgacgaggac cggctggcgc tggtcgtcac caccggcggc accggcctgg ccccgcgcga
     1201 cgtaaccccc gacgcgacgc tggccgtgct tgactaccag gtgccggggc tggccgaggc
     1261 gatgcgcgcc gccagcctgg cccacacgcc gctcgccatg ctctcgcggg cggtggccgg
     1321 ggtgcgcggg cgcacgctga tcgtcaacct gcccggcagc cccaaagcgg tgcgcgagtg
     1381 cctggcggcc atcgcccctg ccctgcccca cggcctggag aaactcgcgg gcgaccccaa
     1441 cgagtgcgcg ccgtagggca ggcctaaccc cctggccccc ttcccggagc gggaaggggg
     1501 aaagatgatt cccttttgcg ggcggcgata aggcgctgtc cggtctggaa tagtcagtat
     1561 gaccgacaag atctcgcgcc agatggcgcg caatgtgatg ctggcggcgc agggcctcga
     1621 ccaattaccc cggcagccgg cgggcaaagc cgacgtgctc gaagcagtgc gccggatgta
     1681 cctgctgcag atcgatacga tccatgttgt ggcccgcagc ccgtacctgg tgctttggag
     1741 ccggttgggc gattatcagc ccgtgtggct ggaccaactg ctggctgaag gcgcgctgtt
     1801 tgagtactgg gcccatgcga tgtgcttcat tcccattgag gattaccccc tctaccgccg
     1861 ccgcatgttg gacgccgtcc ggcaggaggt ctggcccgta aaatgggccg tgaaatgggc
     1921 ccaggaaaac ccgcaggtca tggaacgggt gcgcacccac ctgcgggaga acggcgcggt
     1981 gcggtccgcc gagttcgaga a
//
LOCUS       DASUYC010000666         8166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_673953,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000666 DASUYC010000000
VERSION     DASUYC010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 8166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8166
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673953"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            520..1020
                     /gene="purE"
                     /locus_tag="VF276_10855"
     CDS             520..1020
                     /gene="purE"
                     /locus_tag="VF276_10855"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX6607401.1"
                     /translation="MPDAEETPPLVAVVMGSKSDWETMRHAAETLAQFAVPYERRIVS
                     AHRTPAWLAQFAAEAEGRGLEVIIAGAGGAAHLPGMVAAQTLLPVLGVPVETHALKGM
                     DSLLSIVQMPAGIPVGTLAIGRAGAVNAALLAVAILAGTRPDLRERLRTFRAEQAARV
                     LADTLP"
     gene            1017..2162
                     /locus_tag="VF276_10860"
     CDS             1017..2162
                     /locus_tag="VF276_10860"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005003316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEX6607402.1"
                     /translation="MSGPILPGATIGVLGSGQLGRMLALAAARMGYRVHTFSPERDTP
                     TGQVAAREIVAPYDDLDAVRAFARAVQVVTFEFENVPAATAAAAAEYAPVRPAGAVLH
                     TTQHRLREKTFLAGAGLPVTPFRPVRSLADLTAALAELGCPAVLKTAGFGYDGKGQVL
                     IRTPDAAPSALATLDGQEAILETFVDFAREDSVVAARGLDGAVVDYGLIENTHHHHIL
                     DVSVAPAPGDPALARAAREIAQAVLEALDVVGVLCVEFFETRDGRLLINELAPRPHNS
                     GHLTIDACVTSQFEQHLRAVCGLPLGAPDLLRPAAMANLLGDLWTAGEPDWAASCAFP
                     DVKLHLYGKHATRPGRKMGHLTALAATPEAARARVLAARAALTAPAR"
     gene            2413..2841
                     /locus_tag="VF276_10865"
     CDS             2413..2841
                     /locus_tag="VF276_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEX6607403.1"
                     /translation="MATDIKTTNPTDILTEAEPTLIEMSPVAVEQLQTLLAQQDNPEL
                     ALRVFVYPGGCSGMSYGMAFEDEFSEDDMLFTQGGMRLVVDENSAHYLKGAQIDFEDS
                     LMGGGFRIFNPNAVRSCGCGHSFDTGEDAGSARACGCGGH"
     gene            2988..3794
                     /locus_tag="VF276_10870"
     CDS             2988..3794
                     /locus_tag="VF276_10870"
                     /inference="COORDINATES: protein motif:HMM:NF016155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HEX6607404.1"
                     /translation="MVVQRLAPGWPWRLAVLTPLGVYGFVYPFGIGLLLFGWLPPEAG
                     WVGGCLLVAQGLAMAAWCGLNYGAARGLGAALVIAGAAWGLEALGATTGVPFGAYHYS
                     PALGAMLGPVPAAIPFAWIATVGAAFFTAREALPARLAAGALPVVLGAALTTLQDAVL
                     ETIATRVQGYWTWTASGAPYYGVPWANFATWFGASLILGAVLQALLRPAPARPLRYAA
                     LPPLLYAMSLLMFGLLNWSRGFVLPGALATVLLPFVLWRLHACLSPHWAP"
     gene            3767..5383
                     /locus_tag="VF276_10875"
     CDS             3767..5383
                     /locus_tag="VF276_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607405.1"
                     /translation="MSKPALGTLIGRGLAAALLLLTTACFGSPDSTPSPVAQAPVTPP
                     PARPTATAPLPTATEVAVTGPTASAAPERVTPSTSTPAPSALPTPTTRNPLILQALGA
                     MAAVHSYHYTATVQTFDEGRKLQSEREGDYAAPGGLRWVNVVDNITTTAVLTGSVYYV
                     AVDAGAWTSVTGGVAERAHQLSWQLMARAQDVVEAGRDPATDPDPTIHLTFTLPLDNL
                     PLEARPWQVAQGEVWLGVTDYRVRAYKLYAQEPHYETTERVFLSAYNVPVQIAIPVVD
                     TADTGRHGRLLYAHRGPGGSDTAELRIRDLESGTEQTLPDARGNIYTAAWSPDGRQIV
                     YGAGGSLYVFDLTTNNLLNIGRGDAPAFNPDGTLLAFARGIGQQTHPWTMDALGNNAL
                     RLNDYYAQYLDWAPDGTRVLFSGAPATSDPEEDKAQLFTMAADGSDVQALAPLPGAVT
                     APHWSNAGDRIALIVDGHLGIADADGTHLNVLDSANLNTAPVWSPDDTFLAYANRAGQ
                     GAPSRILIRPVDGGDGQPLTGGDDIPLAWH"
     gene            complement(5519..7480)
                     /locus_tag="VF276_10880"
     CDS             complement(5519..7480)
                     /locus_tag="VF276_10880"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX6607406.1"
                     /translation="MSEADPTKPDGTPQPGQPDSLAPAHEAPAPIAGPVPGQHPDVMP
                     DFVFGHLAAGPETTRALIEALSGVRHQFQIDPLRPRADAPVTIRATTGPSFPAQAVWL
                     YYTADGSRPAGHAGQATTGTAVPMVWRDTEWSELLWDYLSVWEATIPPQPAGAYVRYQ
                     IEAIPMPGSDHAPGFADADPKAAGGLEPVFAYLVDAPTAPAWVHDAVIYHIYLDRFHP
                     GAGGQWRVLEKETGILGGTLRGVTEHLDYIAQLGANTIWLSPVFASPTYHRYDATDLL
                     QVDPALGTNEDLGALVQAAHARGLRVLLDLAANHISNRHPLFQDAQTNPAGPYRDWFT
                     FTCWPAEYVTFFSSKGLPRFNNEYPPARAYMLDAVRFWMERYDIDGYRLDYVLGPSHD
                     FWSDYYRTVKAIKPDSFSVAEATAGANVLRSYEGRMDGALDFTFLSMIRGLIAFETIQ
                     ISAFDRFVTQSARYYSPGFVLPAFLDNHDMNRFLWVAKGDKRKLEVAAAIQFTLAAPP
                     IIYYGTEVGIRQKGDVRASGYGRDVEARGPMLWGAEQDADLLAYYRRLTAIRHAHPAI
                     RHGTRVTLHLDDTARTYAYAQIQSGEIGLLVALNLSGEPRTLHLPLPAGQTLRGTPRD
                     LLNGHPVTADNAGLQIRLPPLSAALVELG"
     gene            complement(7745..>8166)
                     /locus_tag="VF276_10885"
     CDS             complement(7745..>8166)
                     /locus_tag="VF276_10885"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX6607407.1"
                     /translation="LLGLVLAYTAGTLVFSTWNLKGFFDTIPVDLEEAARVDGATPTQ
                     AFFRIMLPLARPALAVTALLGFMAGWGEFITAQTVLFSADKYTAMVGLIGLQSNYRQP
                     WGWFAVGAICMSVPVTVLFLLLQKQLQAGLTLGGVKG"
BASE COUNT         1250 a         2738 c         2855 g         1323 t
ORIGIN      
        1 cgggctcgcg gcaccagcga agcacgacct tggacaagtt acccgcctgc cccacaggaa
       61 acggattttc atacgcgctg gtgtgccgta ggcacatgat tggctcagag tgacaaccac
      121 ggagtattgt cattctgagc ggagcgaaga atctcgttgc gtgcgggtac aagacgagac
      181 tctttgctgc gctcagagtg acaaccacgg agcattgtca ttctgagcgg agcgaagaat
      241 ctcgtcgcgt gcgggtacaa gacgagactc ttcgctgcgc tcagagtgac aaacactctg
      301 tgggagatta ctaagcctcg ttcacgcaag tttatcgtac gcagcaatga cgcgccgggc
      361 acacaaaata agactaacat caataatctt cgcgttcttc gcgccttcgc ggtgagattc
      421 cgctggccgg accgtgactt gctctaaata tgttatcatg ggacaggatc gccgacaagc
      481 gcgatacgcc agcttcatgc aagggagagt gccagccata tgccggatgc cgaggaaacc
      541 ccgccgctgg tggcggtggt gatgggcagc aaatcggact gggagacgat gcgccacgcc
      601 gccgagacgc tggcccagtt cgccgtgccc tatgagcgcc gcatcgtgtc ggcgcaccgc
      661 acgcccgcct ggctggccca gttcgccgcg gaggccgagg ggcgggggct ggaagtgatc
      721 atcgccgggg cgggcggcgc ggcccacctg ccgggcatgg tcgccgcgca gaccctgctg
      781 ccggtgctgg gcgtgccggt ggagacgcac gcgctcaagg gcatggactc gctgctttcc
      841 atcgtgcaga tgccggcggg catcccggtc ggcacgctgg cgattgggcg ggcgggcgcg
      901 gtcaacgcgg cgctgctggc cgtcgccatc ctggcgggca cgcggcccga cctgcgcgag
      961 cggttgcgga ccttccgcgc cgagcaggcg gcgcgcgtgc tggccgacac gctgccgtga
     1021 gcgggcccat cctgccgggg gcgaccatcg gcgtgctggg cagcggccag ttggggcgga
     1081 tgctggcgct ggccgccgcg cgcatgggct atcgggtgca taccttctcg cccgagcgcg
     1141 acacgccgac cggccaggta gccgcccgcg agatcgtcgc cccctacgac gacctggacg
     1201 ccgtgcgcgc gtttgcccgc gccgtgcagg tcgtgacctt cgagttcgag aacgtccccg
     1261 cggccaccgc cgccgctgcc gccgagtatg cgcccgtgcg ccccgccggg gccgtgctgc
     1321 acaccaccca gcaccggctg cgggaaaaga cgttcctggc gggcgccggg ctgccggtca
     1381 cgcccttccg gcccgtgcgc tccctggccg atctgaccgc cgctctggcc gaactgggct
     1441 gcccggcggt gctgaaaacg gcgggcttcg gctacgacgg caagggccag gtgctgatcc
     1501 gcacgcccga tgcggcgcca tccgcgctgg ccacgctgga tgggcaggag gcgatcctcg
     1561 agacgtttgt ggactttgcc cgcgaggact cggtggtcgc cgcgcgcggg ctggacggcg
     1621 ccgtcgtgga ctacggcctg atcgagaaca cccatcacca ccacatcctc gacgtgtcgg
     1681 tggcgcccgc gcccggcgac ccggccctgg cccgcgcggc gcgcgagatc gcccaggccg
     1741 tgctggaggc gctggacgtg gtcggggtgc tctgcgtcga gttcttcgag acgcgcgacg
     1801 ggcgcctgct gatcaacgag ttggcgccgc gcccgcacaa ttcgggccac ctgactatcg
     1861 acgcctgcgt gaccagccag ttcgagcagc acctccgcgc ggtgtgcggc ctgcccctgg
     1921 gcgcgccgga tctcctgcgc ccggcggcca tggcgaacct gctgggcgac ctctggaccg
     1981 cgggtgagcc ggattgggcg gcatcctgcg ccttccccga cgtgaagctg catctgtacg
     2041 gcaaacacgc cacccgccct ggccgcaaaa tgggccacct gaccgcgctg gccgccacgc
     2101 cggaagcggc gcgggcgcgc gttctggccg cccgcgcggc gctgaccgcg cccgcccgtt
     2161 aagccggatg cgggcagggc gccgccgtcc ggcggcctgt ttcgccggcg atccggatcg
     2221 cgcctggcta cgtttctata atcgggctcc caatacgtta gccggcttac ggcgcgggcc
     2281 tggtaattgc tagataatag ggtaggtgaa agcgggcggc gcgccgggca gttgacatcg
     2341 tccgcctgat ttagtatgat ttgggctaga gacttttgcg ctctgtgtac gcaccaatag
     2401 gagataccaa tcatggcgac cgatatcaag actacgaatc ccaccgacat cctgaccgag
     2461 gccgagccaa cgctcatcga gatgagcccg gtggccgtcg agcagctcca gacgttgctg
     2521 gcccagcagg acaatccgga gttggcgctg cgcgtcttcg tctatccggg cggctgctcg
     2581 ggcatgtcct acggcatggc cttcgaggac gagttcagcg aagacgacat gctgttcacc
     2641 cagggcggga tgcgcctggt cgtcgacgag aacagcgcgc actacctgaa gggcgcgcag
     2701 atcgacttcg aggacagcct gatgggtggc ggtttccgca tcttcaaccc gaacgcggtg
     2761 cgctcctgcg ggtgcggcca ctcgttcgac actggtgaag atgccggctc ggcccgcgcc
     2821 tgcggctgcg gcggccacta aaccgcgtcc cctgacccga tcccggttcc tggtgccgcg
     2881 ggcggcagac cggggtatgg gaaagatata cgaacaacga gggtgtgcct taacggcaca
     2941 ccctttttgt tgccgcgcgc gggcgcttct ggtatagtag cgcgacgatg gtggtgcagc
     3001 gattggcgcc gggctggccc tggcggctgg cggtgctcac gccgctgggc gtctacggct
     3061 tcgtctatcc tttcggcatc ggcctgttgc tgttcggctg gctgccgccg gaggccgggt
     3121 gggtcggcgg gtgcctgttg gtggcgcagg ggctggcgat ggcggcctgg tgcggcctga
     3181 actacggcgc ggcgcgcggg ttgggcgcgg cgctcgtcat tgcgggggcc gcctggggac
     3241 tcgaagcgct tggcgccacc accggcgtgc cgttcggggc ctatcactat agcccggcgt
     3301 taggcgccat gctcggcccg gtgccggcgg cgatcccgtt tgcctggatc gccacggtgg
     3361 gcgccgcgtt cttcacggcg cgggaggcgc tgccggcgcg gttggcggcg ggcgcgctgc
     3421 cggtagtgct gggggccgcg ctcaccaccc tgcaagacgc cgtgctggag accatcgcca
     3481 cccgcgtgca ggggtactgg acgtggaccg cgtccggcgc gccgtattac ggggtgccct
     3541 gggccaattt cgccacctgg tttggggcct cgctcatcct cggcgccgtc ttgcaagcgt
     3601 tgttgcgccc ggcgcccgcc cggcccttgc gttatgctgc tctgccgccg ctgttatatg
     3661 ccatgagcct gctgatgttt ggcctgctca actggagtcg gggcttcgtg ctgccggggg
     3721 ccctggcgac ggtgctgttg cctttcgtat tgtggagatt gcacgcatgt ctaagcccgc
     3781 attgggcacc ctgatcgggc gtggcctcgc cgccgcgctg ttgctgctta ccaccgcctg
     3841 cttcggttct cccgactcca caccctcgcc ggtggcccag gcgcccgtca cgcccccgcc
     3901 ggcccggccc acggcaacgg cgcctctgcc gacggccacc gaggttgccg tcaccgggcc
     3961 cacggcctcc gcagccccgg aacgcgtgac cccgtccaca tcgaccccgg ctccgtccgc
     4021 cttgcccacc cccaccacgc gcaacccgct gatcctgcaa gccctcggcg ccatggccgc
     4081 ggtgcacagc taccattaca cggccaccgt gcagacgttt gacgaggggc gcaagctcca
     4141 atctgagcgc gagggcgatt acgcggcgcc gggcggcctg cgctgggtga acgtggtcga
     4201 caacatcacc accaccgcag tgctgaccgg cagcgtgtac tacgtggcgg tggatgcggg
     4261 cgcgtggacc agcgtgaccg gcggcgtggc agagcgcgcg caccagcttt cctggcagct
     4321 catggcccgc gcgcaagacg tggtcgaggc cgggcgcgac ccggctacgg acccagatcc
     4381 gaccatccac ctgaccttca cgctgccgct ggataacttg ccgctggagg cgcggccctg
     4441 gcaggtggcc cagggcgagg tctggctcgg cgtgaccgac tatcgcgtgc gcgcctacaa
     4501 gctgtatgcg caagaacccc actacgagac gaccgagcgc gtgttcctct cggcctacaa
     4561 cgtcccggtg caaatcgcca tcccggttgt cgatacggcg gatacgggcc ggcacggccg
     4621 gttgctctac gcgcaccgcg ggccgggggg ctcggatacg gcggaactgc gcatccgcga
     4681 cctggagtcg ggcaccgagc aaaccctgcc cgacgcccgc ggcaacatct ataccgcggc
     4741 ctggtcgccc gatgggcggc agatcgtcta cggggcgggc ggcagcctct acgtgttcga
     4801 tctgaccacg aacaacctgc tgaacattgg ccggggtgat gcgcctgcct tcaaccccga
     4861 cggtacctta ctggccttcg cccgcggcat cggccagcaa acccatccct ggaccatgga
     4921 cgccctgggc aacaatgcgc tccggctgaa cgactactat gcccagtacc tggactgggc
     4981 gcccgacggc acgcgcgtcc tgttcagcgg cgccccggcc acgagcgacc cggaagaaga
     5041 caaggcgcaa ctctttacca tggccgccga cggcagcgat gtccaggctc tcgcgccgct
     5101 gcccggcgcg gtcacggcgc ctcactggtc gaacgccggc gaccgcatcg ctctgatcgt
     5161 cgatgggcac ctgggcatcg ccgacgcgga cggcacgcac ctgaatgtgc tggacagcgc
     5221 gaacctgaac accgcgcccg tgtggagtcc cgacgacaca ttcctggcct atgccaaccg
     5281 ggccggccag ggcgcgcctt cgcgcatcct catccgcccg gtggacggcg gcgacgggca
     5341 gccgctcacc ggcggcgacg acatcccgct ggcctggcac tgaccggcgc ctcgcctttg
     5401 tcactctgag cggaccgaag agtctcgtcg tttggacacg agacggagat tcttcgctgc
     5461 gctcagaatg acggatgtga attgtcactc tgagcggagc gaagagtctc gtggggtttc
     5521 agcccagttc gaccagggcg gcggacaggg gcggcaggcg gatctggagg ccggcgttgt
     5581 ctgccgtgac agggtggccg ttcagcaggt cgcggggggt gccgcgcaag gtctgcccgg
     5641 cgggcagcgg caggtgtagc gtgcgcggct ccccgctcag gttcaaggcc accagcaggc
     5701 cgatctcgcc cgattggatc tgcgcgtagg cgtaggtgcg cgccgtgtcg tccaggtgca
     5761 gcgtcacgcg ggtgccgtgg cggatggcgg ggtgggcgtg gcggatggcg gtcagccgcc
     5821 ggtagtacgc cagcagatcc gcgtcctgct cggcgcccca gagcatcggg ccgcgcgctt
     5881 ccacatcgcg gccgtacccg ctcgcgcgca cgtcgccctt ctggcggata cccacctcgg
     5941 tgccatagta gataatcggc ggggcggcca gtgtgaactg gatcgcggcc gccacctcca
     6001 gtttgcgttt gtcgcctttt gccacccaca ggaagcggtt catatcgtgg ttgtcgagaa
     6061 acgccggcag cacgaagccg ggcgagtaat agcgcgcgct ctgcgtcacg aagcggtcga
     6121 aggcgctgat ctggatggtc tcaaaggcga tcaggccgcg gatcatgctc agaaacgtga
     6181 agtcgagcgc gccgtccatg cggccctcgt agctgcgcaa cacattggcc ccggcggttg
     6241 cctcggccac cgagaagctg tcgggcttga ttgccttcac cgtgcggtag tagtcgctcc
     6301 agaaatcgtg cgacgggccg agcacatagt cgagccggta gccgtcgatg tcgtagcgct
     6361 ccatccagaa gcgcacggcg tcgagcatgt aggcgcgcgc cgggggatac tcgttgttga
     6421 agcgcggcaa ccctttggaa ctgaagaacg tgacgtactc ggcgggccag caggtaaagg
     6481 tgaaccagtc gcggtaaggg ccggcggggt tggtctgcgc gtcctggaac aacgggtggc
     6541 ggttggagat gtggttggcg gccaggtcga gcagcacgcg caggccgcgg gcatgggccg
     6601 cctggaccag cgcgccgagg tcctcgttcg tgcccagcgc cgggtccacc tgcaacaggt
     6661 cggtggcgtc gtaacgatgg taggtcgggc tggcgaagac gggcgacagc cagatcgtgt
     6721 tggcccccag ttgcgcgatg taatcgagat gctcggtgac gccgcgcagc gtgccgccca
     6781 ggatgccggt ctctttttcg agcacgcgcc attgcccgcc ggctcccgga tggaagcggt
     6841 cgagatagat gtggtagatg acggcgtcgt ggacccaggc gggcgcggtg ggtgcgtcga
     6901 ccagataggc aaagaccggc tccaggcccc cggcggcttt cgggtcggcg tcggcgaagc
     6961 ccggcgcgtg gtcgctgccc ggcatgggga tcgcctcgat ctggtagcgc acatacgcgc
     7021 ccgcaggctg cggcgggatc gtcgcctccc acaccgacag gtaatcccag agcagttcgg
     7081 accattcggt gtcgcgccag accatcggca ccgcggtgcc ggtggtcgcc tgtccagcgt
     7141 gcccggcggg gcggctgccg tcggcggtgt agtagagcca gacggcctgc gccgggaagc
     7201 tcggccccgt cgtggcgcgg atcgtcaccg gggcatccgc ccgcgggcgc aagggatcga
     7261 tctggaactg atggcgcacg ccgctgaggg cctcgatcag ggcgcgggtg gtttccggcc
     7321 cggcggccag gtggccgaac acaaagtcgg gcatgacgtc ggggtgctgg ccgggcaccg
     7381 ggcccgcgat gggcgcgggc gcctcgtgcg ctggggccag cgagtcgggc tgccccggtt
     7441 ggggggtgcc gtcgggtttg gttgggtcag cttcgctcat aacacgtgct cacaaagaga
     7501 gggaggcaac ccaccggtta ctccctctcg actaaggcgg aattgaagtc tagcgggccg
     7561 ggccggtttc aaaccggccc ttacaccgca taattgccat gacgggccgg tttgaaaccg
     7621 gcccctacac cgcaggattg ccatgacggg ccggtgtgaa accggtcctt acaccgcagg
     7681 attgccatga cgggccggtg tgaaaccggc ccctacacca ggcgcaaggg tgggggcgcc
     7741 ggccttagcc cttcacgccg ccgagggtca gcccggcttg caactgcttc tgcagcagca
     7801 ggaacagcac cgtgaccggc accgacatgc agatggcgcc caccgcgaac cagccccacg
     7861 gctggcggta gttgctctgc aagccgatca ggcccaccat ggcggtatat ttgtcggcgc
     7921 tgaacagcac cgtctgcgcc gtgatgaact cgccccagcc ggccatgaag cctagcagcg
     7981 ccgtgacggc cagggccggg cgggccagcg gcagcatgat gcggaaaaac gcctgggtgg
     8041 gcgtggcccc gtccacccgc gccgcctctt ccaggtccac cgggatggta tcgaagaagc
     8101 ccttcaggtt ccaggtcgag aagaccaggg tgcccgccgt gtaggccagt accaacccca
     8161 gcaggt
//
LOCUS       DASUYC010000667         3160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1966336, whole genome shotgun sequence.
ACCESSION   DASUYC010000667 DASUYC010000000
VERSION     DASUYC010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1966336"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..1643)
                     /locus_tag="VF276_10890"
     CDS             complement(90..1643)
                     /locus_tag="VF276_10890"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6607408.1"
                     /translation="MFRRLPPILLLTILLVPALAVPAPPAGAAPAPRPPQPAAALPGT
                     PMCFVQTGYCLRGSFLLYWRVNGGLPQFGFPVTPEITEDGRTVQYTERARFELHDNNT
                     VLLGLLGRAVTQNRTDSPFRPATAAAGATYYRETSHNLAEPFRAYWQDTGGLPVYGYP
                     ISEAFQERSPTDGKTYLVQYFERHRLEYHPEAGADARIQLGLLGSQVYAHSYGNLPPL
                     KPNPALAPDPVSLARLRQAPRTGRDLQVTRTIARTAAYTQYGITYRSAGRRISGVMYV
                     PAGKGPFPVIIMNHGFIPISQYTTGMDSRRESPFLASHGYVAIHPDFRNYAGSDDDPD
                     AAGNMTTIGWTEDALNLLTAVQQSSLPYLDASRIGMWGHSNGGQVGLQVMSIDVDIKA
                     YVLFAPTSPDYADNFNRWSRNSAEARAIKAAHGFPEDNPEFYRGISSGPWFDQVSAPV
                     LIFHGTGDTTTPYAWSVRTVDLLRAAGKDITFVSPKGENHLFSDRAWSTGGIGAQMLA
                     FFDQHVKGR"
     gene            1934..>3160
                     /locus_tag="VF276_10895"
     CDS             1934..>3160
                     /locus_tag="VF276_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607409.1"
                     /translation="MGEESLKVAQQWKGLRIGGALTWQAALLFLVSILPVLVIVGWFN
                     YQHVSTTVQERIGRDYTSRVSSMESELSNVLLRELERASTLTRAPGMRDLPPPARTET
                     DTALQQAYEQAAPSAAVRLTYIDNAAGRLLKDFREQFPNRAVVLLTDAQGRLEGVTTP
                     AWPYWNVARQAWWPDMARLGRDAYSISSPIDMPGFGSLLFLTAPVLDADDKPSGLLVV
                     GLRFPDMVGPILGQDLNESPGSTAMLVNEDGRVLYTTPAWPAAQLPATWAPVLQPVEW
                     NTRTVDANLVAYAPMQVAEGYALDDPTSIRTLNRLNWRVLQIIPTNIAYAAVDEELRL
                     LSLGTVATALVIVLLAVIGVRIVVTKPLQRLDLIIADVERHGLANEVAVRGRGRLPRS
                     HNEIGRLGQRFGRMLDA"
BASE COUNT          517 a          989 c         1121 g          533 t
ORIGIN      
        1 gatcaacccg tccgaaagtg cggaggcggc ccgatggggc cgcctcttgc tgcatggcgc
       61 aggctgctgc ccggttcccc gccgagcggc taccggcctt tcacgtgctg gtcgaagaag
      121 gccagcatct gcgcgccgat gccgcccgtg gaccaggcgc ggtcgctgaa caggtggttt
      181 tcgcccttag gcgagacgaa ggtgatgtcc ttgcctgccg cgcgcagcag atccaccgtg
      241 cgcaccgacc aggcgtaggg cgtggtggtg tcgccggtgc cgtggaagat gagcaccggg
      301 gcgctgacct ggtcgaacca ggggccggac gagatgccgc gatagaactc cgggttgtcc
      361 tccgggaagc cgtgggcggc tttaatcgcc cgcgcctccg cgctgttgcg cgaccagcgg
      421 ttgaaattat cggcgtaatc ggggctggtc ggcgcgaaga gcacataggc tttgatgtcc
      481 acgtcgatgg acatgacttg caggcccact tgcccgccgt tggagtgccc ccacatgcca
      541 atgcggctcg cgtccaggta gggcagggac gattgctgca cggcggtgag aaggttcagc
      601 gcgtcttcgg tccagccgat ggtggtcatg ttgcccgcgg cgtcggggtc gtcgtccgat
      661 ccggcgtaat tgcggaagtc ggggtggatg gcgacgtagc cgtggctcgc caggaagggc
      721 gactcgcggc gcgagtccat gccggtggtg tattgcgaaa tcgggataaa gccgtggttc
      781 atgatgatga ccgggaaggg gcctttgccc gccgggacat acatcacgcc ggagatgcgg
      841 cggcccgcgc tgcggtaggt aatgccgtac tgggtatagg ccgcggtgcg ggcgatggtg
      901 cgcgtgacct gcaaatcgcg gcccgtgcgc ggggcctggc gcagccgggc cagcgacacc
      961 gggtcgggcg ccagggcggg attgggcttg agcgggggca ggttgccgta gctgtgcgcg
     1021 tagacctggc tgcccagcag gccaagctgg atgcgcgcat ccgccccggc ctccgggtga
     1081 tattccagcc ggtggcgctc aaaatactgc accaggtacg tcttgccgtc ggtggggctg
     1141 cgttcctgga aggcttcgct gatgggatag ccgtagaccg gcaagccgcc ggtgtcttgc
     1201 cagtaggcgc ggaacggctc ggccaggttg tggctcgttt cgcggtagta ggtggcgccc
     1261 gcggcggcgg tggccgggcg gaaggggcta tcggtgcggt tctgcgtcac ggcccggccc
     1321 agcaggccga gcagcacggt gttgttgtcg tgcaactcga agcgggcgcg ctcggtgtat
     1381 tgcacggtgc gcccgtcttc ggtgatctca ggcgtgaccg ggaacccgaa ttgcggcagc
     1441 cccccgttca cgcgccagta aagcagaaaa ctgccgcgca ggcagtagcc cgtctggacg
     1501 aagcacatgg gggtgccggg tagcgctgcg gcgggctgcg gcgggcgcgg tgcgggcgcg
     1561 gccccggcgg gcggcgccgg cacggcgagc gccggcacca gcaagatggt cagcagcagg
     1621 atcgggggta gacggcgaaa catggcggct ccttggcagg gacgacggtt ggcggcacga
     1681 tacgcaccgc attttgctcc cactataccc aatctttacc atttgggcca gtctccccgg
     1741 caggcacatc gaaacggcgt cctatagtag actaaggtcc ctggggaatg ggactggtag
     1801 gacattgtat cggatcgaac cattccccta tactgctgat aaatcgacgt gatccgaacc
     1861 tgatcgcggg cagtgatcat cccagggccg cccatggccg gccccgatgc ccggcggact
     1921 atggaaccac gcaatgggag aagagtcgtt gaaggtagca caacagtgga agggattacg
     1981 catcggcggc gcgctgacct ggcaggccgc cctgttattt ctggtcagca tcttgcccgt
     2041 gctggtgatc gtcggctggt tcaattacca gcatgtctcg acgaccgtgc aggagcgaat
     2101 cggccgggac tataccagcc gcgtgagcag catggaatcg gagctatcca atgtgctgct
     2161 gcgcgaattg gagcgggcct ccacactcac ccgtgcaccg ggcatgcgcg acctcccgcc
     2221 gccggcccgc acggagaccg atacggcgct gcagcaagcc tatgagcaag ctgcgcctag
     2281 cgccgccgtc cggctgacct acatcgacaa cgccgccggc cggttgctca aggacttccg
     2341 cgaacagttc ccgaaccggg cggtcgtgct gctgaccgat gcgcaagggc ggctggaggg
     2401 cgtcaccacc ccggcctggc cgtactggaa cgtcgcgcgc caggcctggt ggcccgacat
     2461 ggcccgcctg ggccgcgatg cctattcgat ttcctcgccc atcgacatgc ctggcttcgg
     2521 cagcctgctg ttcctcacgg cgccggtgct cgacgcggat gacaaaccgt ccggcctgct
     2581 ggtagtgggg ttgcggttcc ccgacatggt ggggccgatt ctcggccagg atctgaatga
     2641 gagtccgggc agcacagcca tgctggtcaa cgaggacggt cgggtgctct acacgacgcc
     2701 ggcctggccg gcagcccagc taccggcaac ctgggcaccc gtcctccagc ccgtcgagtg
     2761 gaatacccgc accgtggacg ccaacctcgt cgcctacgcg cccatgcagg tggccgaggg
     2821 ctatgctctg gacgacccga ccagcattcg cacgctcaac cggctcaact ggcgcgtgct
     2881 gcagattatc cccacgaaca tcgcctacgc agccgtagac gaggaattgc ggctgttgag
     2941 cctgggcacc gtcgcgacgg cgctggtcat cgtgctgctg gccgtgatcg gggtgcgcat
     3001 cgtagtcacg aagccgctgc aacggctcga tctgattatc gccgatgtcg agcgccacgg
     3061 cctcgccaac gaagtggccg tgcggggccg ggggcggctg ccacgcagcc acaacgagat
     3121 cggccggctg gggcagcgct ttgggcgtat gctcgacgcg
//
LOCUS       DASUYC010000668         6020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1545366, whole genome shotgun sequence.
ACCESSION   DASUYC010000668 DASUYC010000000
VERSION     DASUYC010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6020
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1545366"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="VF276_10900"
     CDS             complement(<1..856)
                     /locus_tag="VF276_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013555296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-opening dioxygenase subunit LigB"
                     /protein_id="HEX6607410.1"
                     /translation="METAKLQGGLVFACIAPHGGMVVPELAGAERDLAAQTRAAMEDL
                     GRRMAAARPDTVVVITPHGIRVSGLMCVSVTETAFGDLNDDDENPQGHIELSLTIDRD
                     LAYAIAEAAHAKDVPVGLVGYGSSSGEASCLPLDWGALIPLWFMGGRATPPPEIVVVT
                     PSRQLPLEQLVAFGRSIAEAAAESGKRVGLIASSDLGHAHQADGPYGYDPASARFDKL
                     VADAVRAEDLDRLMHLDEDFIEAAKPDGLWQIVILKGAIEHTPMRGELLSYQAPTYFG
                     MLCAAYAPD"
     gene            1098..2645
                     /locus_tag="VF276_10905"
     CDS             1098..2645
                     /locus_tag="VF276_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013919633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6607411.1"
                     /translation="MSKKRWGMLIPMIVMLSLLLAACGAEPTATTAPAAATDTPAAAA
                     AATDTPAAAAPTNTTAAAAPTDTPAAAAATATEATAGTTPTTGGAPAKTDYSKVGPEL
                     VNAFAGQYKGTKVSMFGPFTGDDEVKFNNSMKDFEQATGIDIQYVGDKQFETTINVRV
                     EGGSPPDIADFPQPGLLASIAKSGKVLDANKLVNPDWLKQNYNQGYLDTATVDTPSGK
                     ILGGIFQRVNAKGLVFYPKKAFDAAGYKTPTTWDEQTKLMNDIVADGDTPWCIGIASG
                     TATGWPATDWVENIMLRTTSTENYDKWTRGELPFTAPEVKKAVQMMADIWFNDKYVYG
                     GRQQIVSTDFGASVAPMFQDPPKCWLVNQGNFITSYFETAKPGVKGGVDYDFYYLPPI
                     DPQYGKPVEFGGDIMTAFNDRPEVRAVLQYFSTFDGVKGWVQQGGAIAPQNDSDLAAY
                     GSDMDRKVAQTLLGASVVRFDGSDLMPGAVGAGTFWKAMTDFVSGNANIDQALQEAQA
                     GWASVKK"
     gene            2796..3911
                     /locus_tag="VF276_10910"
     CDS             2796..3911
                     /locus_tag="VF276_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019637016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX6607412.1"
                     /translation="MQGTIELNSEKTPPPAVGGWAPGRFFAALVGLIVTLAILWFGFD
                     FLRSNNLNGLVAGLIAIIWGVGGVAALFTVANMLVEALPEGWRALLQPYVFVGPGVLL
                     LAAFLLIPLLLTIYQSLFGPSSTDFVGLSNYASVFTDRGMLESFRNNLLWLFLGTAAC
                     VILGLLIAVLADRSKFEAVAKALIFLPMAISFVGAGVIWRFVYDYAPPGEQQIGLLNA
                     IVVAFGGQPQGWVTTEPWNNLFRIAVLVWMQTGFAMVLFSAALKGVPGDLLEAARIDG
                     ATEVQAFFRIIIPYIQGTIITVTTTIAIFTLKIFDVVIVMTGGQYGTEVVATEFYRQF
                     FTNRNFGYGSAIAIVLFIAVIPVMVYNLMQLRKQEGF"
     gene            3911..5053
                     /locus_tag="VF276_10915"
     CDS             3911..5053
                     /locus_tag="VF276_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018751082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX6607413.1"
                     /translation="MAALQPAVATTQQRAVPTRRQFPWGQVLVNLVLALICLVWTIPT
                     IGLLISSVRPREDISTSGWWTVFPHQAWTSTNQVQLEKGIALDQPVTVNGVSVTDDQL
                     RAGYKLPNGDRLIWANRRNRLVAVQSQQWTMNLNFNLDNYQSVLVGQQYKYTLPDGTV
                     RVDQGQDLSAAFITTIVVAVPATVIPILIAAFAAYGFAWTRFRGRAIMFAMVVALLVV
                     PLQVALIPLLRAYTALGVNGTYLALWLAHTGFGLPLATYLLYNYISTLPREVLESAFI
                     DGASQYTIFTRLILPLSVPALASFAIFQFLWVWNDYLVALIFLGTQGGGQQVLTMRLA
                     GISGSRGQDWYLLTAGAFISMVLPLTVFFALQRYFVRGLLAGSVKG"
     gene            5170..5445
                     /locus_tag="VF276_10920"
     CDS             5170..5445
                     /locus_tag="VF276_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607414.1"
                     /translation="MSDQPLFQNMDEEERIYAPEQLPPEEQDRVRADEGRVRVDPEEP
                     PAAAPVANMGNAPSAAMAPPSDEFAQLNAVSTDPDINPANQIDPDRQ"
     gene            5680..>6020
                     /locus_tag="VF276_10925"
     CDS             5680..>6020
                     /locus_tag="VF276_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607415.1"
                     /translation="MIAHHAEIALQPGVVVAPLSDLAGVQAPDGRLLSPERLGLGLVQ
                     TCAPGGDVCVRWLDAGLDLWVAGEELRVLAAGRHVVSLWRRDQQGRRFLRGRRLAALQ
                     HDWTVELLPRR"
BASE COUNT         1034 a         1995 c         1859 g         1132 t
ORIGIN      
        1 cgtccggcgc gtaggcggcg cagagcatcc cgaagtaggt cggcgcctgg tacgacagca
       61 gctctccgcg catcggcgtg tgctcgatgg cgcccttcag gatcacgatc tgccagaggc
      121 cgtcgggctt ggccgcttcg atgaaatcct catcgaggtg catcagccgg tccaggtctt
      181 ccgcgcgcac ggcatcggcc accagcttat cgaagcgggc cgaggccggg tcgtagccgt
      241 aggggccgtc agcctggtgg gcgtggccca ggtccgaact ggcgatcagc ccgacccgct
      301 tgcccgactc cgccgccgcc tcggcgatgc tgcgtccgaa cgccacaagc tgttccagcg
      361 gcaactgccg cgacggcgtc accaccacga tctcgggcgg cggcgtggcc cgcccgccca
      421 tgaaccagag cgggatgagc gcgccccaat cgagcggcaa gcaactcgcc tcgccgctgc
      481 tcgacccgta gcccaccagg cccaccggca cgtccttcgc atgcgcggcc tcggcgatgg
      541 cgtaggccag atcgcggtcg atggtcaggc ttaattcaat gtgcccttgc gggttctcgt
      601 cgtcgtcgtt caggtcaccg aaggccgtct cagtcacgct cacgcacatc aagccactga
      661 ctcgaatgcc gtggggtgtg ataacgacca ccgtgtcggg ccgggccgcc gccatgcgcc
      721 ggcccaggtc ctccatggcc gcgcgcgttt gcgcagccag gtctcgctcc gcgcccgcga
      781 gttcgggcac caccatcccc ccatgcgggg cgatgcaggc gaagaccagt cctccttgca
      841 attttgccgt ctccatagta agataagctc tccttattgc cggctccggc gcattgcgcc
      901 atcctttgtc gccgattata ccatacggct gggtattgtt cttgctgcat atggatgtat
      961 aatggcaagg gctttaccag gaggccccca cacttgaccg tggaggaggt ggttaaggtt
     1021 agaaccgggc caacgcatct aacggacttc accgtattaa ccccatccgt tttgtcccaa
     1081 ggaggagagt aattaccatg tctaaaaagc gttggggaat gctgatcccc atgatcgtga
     1141 tgttgagcct gttgctggcg gcgtgcggcg ccgagcctac ggcgaccacg gctcccgccg
     1201 cggccactga taccccggct gccgccgccg cggccactga tacgcctgct gccgcggccc
     1261 cgaccaatac cactgcggcc gccgcaccca ccgatacgcc ggctgctgcc gccgccacgg
     1321 ccaccgaggc cacggccggt accacgccga ccactggcgg cgcccccgcg aagaccgatt
     1381 acagcaaggt gggccccgaa ctggtcaatg cctttgccgg ccagtacaag ggcaccaagg
     1441 tcagcatgtt tggccccttc acgggtgacg acgaggtcaa gttcaacaac tcgatgaagg
     1501 acttcgagca ggctaccggc atcgatattc agtatgtcgg cgacaagcag tttgaaacga
     1561 cgatcaacgt ccgcgtcgag ggcggcagcc cgcccgatat cgccgacttc ccgcagccgg
     1621 gtctgctcgc cagcatcgcc aagtcgggca aagtgctgga tgccaacaag ctggtcaacc
     1681 cggactggct gaagcagaac tacaaccagg gttatctgga taccgccacc gtcgacaccc
     1741 cgagcggcaa gatcctgggt ggcatcttcc agcgtgtgaa cgccaagggc ctggttttct
     1801 acccgaagaa ggcgtttgac gccgccggct acaagacgcc cacgacctgg gatgagcaga
     1861 cgaagctgat gaacgatatc gtcgctgatg gcgacacccc gtggtgcatc ggtatcgcca
     1921 gcggcacagc caccggctgg ccggccaccg actgggttga gaatatcatg ctgcgcacca
     1981 cctcgacgga gaactacgac aagtggacgc gcggcgagct gccgttcacc gcgcccgaag
     2041 tcaagaaggc cgtccagatg atggccgaca tctggttcaa cgacaagtat gtctatggcg
     2101 gccgccagca gatcgtcagc accgatttcg gcgcctccgt ggctcccatg ttccaggatc
     2161 cgccgaagtg ctggctggtc aatcagggca acttcatcac cagctacttc gagacggcca
     2221 agcccggcgt caagggcggc gtggattacg acttctatta tctgccgccc atcgacccgc
     2281 agtacggcaa gccggtggag ttcggtggtg acatcatgac cgccttcaac gaccggcccg
     2341 aagtgcgcgc ggtgctccag tacttcagca cctttgacgg cgtcaagggc tgggtgcagc
     2401 agggcggcgc tatcgcgcct cagaacgact ccgatctggc cgcgtacggc agcgacatgg
     2461 accgcaaggt cgcccagacc ctgctcggcg ccagcgttgt ccgcttcgac ggctccgacc
     2521 tcatgccggg cgccgtcggc gccggtacct tctggaaggc catgaccgac ttcgtcagcg
     2581 gcaacgccaa catcgatcag gcgttgcagg aggcgcaggc gggctgggcc agcgtcaaga
     2641 agtaagccgt cgggctgatc ggaatccgcg tggcgattgt cgccgggaac tctccccggc
     2701 ggcaatcgcc acacaatata gaaaccgggg tggccttgtc ctgaaatgca ctgcgagtcc
     2761 ggatgcccgt cgtcctcaat gacaggagat ctgagatgca aggtacgatt gaactgaatt
     2821 cagagaaaac cccgccgccc gctgtaggag gttgggcgcc cggacgattc tttgccgccc
     2881 ttgtgggcct gatcgtcacg ctggcgatcc tctggttcgg gtttgacttc ttgcgcagca
     2941 acaacctcaa tggcttggtt gccgggctga ttgccatcat ctggggcgtc ggcggggtcg
     3001 cggccctgtt caccgtggcg aatatgctcg tcgaagcgct acccgaaggc tggcgcgcgc
     3061 tcctccaacc ttacgtcttt gtcggtcccg gtgtattgtt actggcggcc ttcctgttga
     3121 tccccctgct cctcaccata tatcagagtc tctttggtcc cagttcgacc gactttgtcg
     3181 gcctgagcaa ttatgcatcc gttttcaccg accggggaat gctcgaatcc ttccgcaaca
     3241 acctgctctg gcttttcctc ggaaccgccg cctgcgttat actgggattg ctgatcgcgg
     3301 ttctcgcgga tcgcagtaaa tttgaggcgg tcgccaaggc cctcatcttt ctgcccatgg
     3361 cgatctcctt cgtcggcgct ggggtgatct ggcgcttcgt ctacgactat gcgccgccgg
     3421 gcgaacagca aatcggcctg ctgaacgcaa tcgtggtcgc gtttggcggg cagccgcaag
     3481 gctgggtcac cactgagccg tggaacaacc tgttccggat cgccgtgctc gtctggatgc
     3541 aaactggctt cgccatggtc ctcttctcag cggccctcaa aggtgtgccg ggcgatctgc
     3601 tcgaagccgc ccggatcgac ggggccaccg aggtccaggc tttcttccgt attattattc
     3661 cctatatcca gggcaccatc atcaccgtca ccaccactat tgctatcttc accctgaaga
     3721 ttttcgatgt ggtcatcgtc atgaccggcg gccagtacgg cacggaggta gtggcaacgg
     3781 agttctaccg ccaattcttc accaaccgca actttggcta tggctccgca atcgccatcg
     3841 tgctcttcat tgcggtgatc cccgtgatgg tctacaacct gatgcaatta cgcaaacagg
     3901 agggattcta atggccgccc tacaacctgc cgtagcgacc acccagcagc gcgccgtgcc
     3961 cactcgccgc cagttcccct ggggccaggt cctggtgaac ctcgtgctgg cgctgatttg
     4021 tctggtgtgg accatcccga cgatcggtct cctgatctcc tcggtgcgcc cccgcgagga
     4081 catctccacc tctgggtggt ggacggtgtt cccgcatcag gcctggacaa gcaccaacca
     4141 agtgcaattg gaaaagggca tcgcgctgga tcaaccggtc acggtcaacg gcgtgagcgt
     4201 gaccgacgac caactacgcg ccgggtataa gctccccaac ggcgatcggc tcatctgggc
     4261 gaaccgccgc aaccggctcg tggctgtgca gagccagcag tggacgatga acctcaactt
     4321 caatctggac aattaccaga gtgtgttggt cggccagcag tacaaataca cgctgcccga
     4381 cggcaccgtg cgcgtggatc agggccagga cttgagtgca gccttcatca ccacgatcgt
     4441 ggtcgccgtg ccggccacgg tgatcccgat cctgatcgcc gcgtttgccg cctacgggtt
     4501 cgcctggacg cgcttccgcg ggcgcgcgat catgttcgcc atggtggtcg cgctgctggt
     4561 cgtgcccctc caggtggcgc tgatcccctt gctgcgcgcg tatacggcgc tgggcgtcaa
     4621 cggcacttac ctggccctgt ggctggcgca caccggcttc ggcttgccgc tggcgaccta
     4681 cctgctctac aactacatca gcacgctgcc gcgcgaggtg ctggagtcgg cgtttatcga
     4741 cggcgcgtcg cagtatacca tcttcacgcg gctgatcctg ccgctgtcgg tgccggcgct
     4801 cgcgtcgttc gccatcttcc agttcctctg ggtctggaac gattacctgg tggcgttgat
     4861 cttcctgggc acgcagggcg gcgggcagca ggtgctgacc atgcgcctgg ccggtatctc
     4921 cggttcgcgc gggcaagact ggtatctgtt gacggcgggc gccttcatct ccatggtctt
     4981 gccgctgacg gtgttcttcg cgctccagcg ctacttcgtg cgcgggttgc tggccggctc
     5041 ggtgaagggc taggcggcat ccgggacgcg ccccggcagg gccggcctgt gggagcacgc
     5101 cccggtggtc gtctatcggc aatggctgca tgatgctgca gctcaaggat tccaagtaag
     5161 gaggcaacca tgagcgatca gccgttattc caaaatatgg acgaagaaga acgcatctac
     5221 gcgccggaac agttaccgcc ggaggagcaa gatcgcgtac gcgccgacga gggccgggtg
     5281 cgcgtggacc cggaggaacc ccccgccgcc gcgcccgtgg cgaacatggg taacgcgccg
     5341 agtgcggcca tggcgccgcc cagcgacgag ttcgcgcagt tgaacgcggt ctccaccgac
     5401 ccggatatca acccggccaa tcaaatcgat cccgatcgcc agtagcgggc ccgtgccggc
     5461 gccgtgaccc accaccgggc tgcgttccgg cccggcggcg tcaggtgccc gccgcacgaa
     5521 ccaccgagtc cccggcggat cgctccgtgc ggggactcgt ggctttccag ggtacgcctg
     5581 ggggccgctc ccccccgtgg gtccggcctg tcaccacccg cctcttccgt cgcctcgcac
     5641 ccttgtagta taatatgagc gaagcgcgag ggtatcgcca tgattgcaca ccatgcggaa
     5701 attgccttgc agccaggggt cgtggtggcg ccgctgagtg atctggccgg ggtccaggcc
     5761 ccggacggcc ggctcctatc ccctgagcgc ctgggcctcg gcctggtcca gacatgtgct
     5821 ccgggcggcg atgtctgcgt ccgctggttg gatgccggac tggatctgtg ggtggccggc
     5881 gaggaattgc gggtgctggc tgccggcagg cacgtagtca gcctctggcg gcgcgatcag
     5941 caggggcggc gcttcctgcg gggccggcgc ctcgccgcgc tccagcacga ctggacggtt
     6001 gaacttctgc cccgccgcat
//
LOCUS       DASUYC010000669         2051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_26760,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000669 DASUYC010000000
VERSION     DASUYC010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26760"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..438
                     /gene="rpmB"
                     /locus_tag="VF276_10930"
     CDS             235..438
                     /gene="rpmB"
                     /locus_tag="VF276_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEX6607416.1"
                     /translation="MAGTGTCDICGKHSMFGHNVSFSARATNRRWAPNIQSAYLNVNG
                     VNKKVKVCTRCLRTLSKTDKTAK"
     gene            610..1281
                     /locus_tag="VF276_10935"
     CDS             610..1281
                     /locus_tag="VF276_10935"
                     /inference="COORDINATES: protein motif:HMM:NF037677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX6607417.1"
                     /translation="MDGKQRATREPEKQYRRRMILDTAAQLLDERAYTAITMAEVAEQ
                     AGLAKGTVYLYFPTKEALFLALQQRMLAEWFAELDAALAQLGPASAGAVAETICRSLA
                     PRPHLTRLLAILHSVLEQNIDYETAVSFKQMLLAHMQRTGARLERCLPFLRPGQGAQV
                     LLRAHALVIGLQHVSDPPPLVRQVLEQPELQVFHIDFAGELSATLRALLRGMECVPGD
                     DAARQ"
     gene            1300..>2051
                     /locus_tag="VF276_10940"
     CDS             1300..>2051
                     /locus_tag="VF276_10940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008187946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6607418.1"
                     /translation="MAKQAQAHRGETALITGASSGFGAEFAKLFARDGYNLVLVARSE
                     GALNTLADQLRTRYGVQVRVLPKDLALPAAPDEIFATLCDERITVDVLVNNAGFATYG
                     PFVEIPLQADMDLLKVNMLALTHLTKLFLPGMLARRRGKILNVASTAAFQPGPLMAAY
                     YASKAYVLFLSEAIAEEVRGSGVTVTALCPGPARTGFQRRAQMEDSRLVSGRIMDART
                     VAVAGYRALQQGHTVVIPGLRNRIFALAPRVSP"
BASE COUNT          361 a          685 c          651 g          354 t
ORIGIN      
        1 ggcgggtgtg ggtggtttca ggcaaactgg atggcccctt ccacaccatg accgccggtg
       61 cgcaatcggc acgggacggc ggtcagcttg catcaaatgc caaattgtgg tattattgac
      121 cttcggagcc gccttcggcc atgcgcgggg ttcgactccc gacagcttga cgctgtgtta
      181 atggtggctt aatcatacag caaaaacctt gtttagtaaa gggggtcgat tccattggcc
      241 ggtaccggta cttgtgatat ttgcgggaag cattcgatgt ttgggcacaa cgtcagcttc
      301 tcggcgcggg ccacgaaccg ccgctgggcg ccgaacattc agtcggcata tttgaacgtg
      361 aacggtgtga acaagaaggt caaggtttgc acgcgctgcc tgcgcacgct gagcaaaacg
      421 gacaagaccg ccaagtaagg cactttctgc aacgtatata agggccgcgc catgaggcgc
      481 ggcctttttg cgctttccgc ccccgcgcta cccacccgat cttggtggtc cctgcctatt
      541 gacaactgac cgtaggtcat ttatcattga cccgtggtca gttaaagaac gagaggacag
      601 cagcgaccca tggacggcaa gcagcgcgcc acgcgggagc cggagaagca gtaccggcgg
      661 cgcatgatcc tggataccgc agcgcaactg ctggacgagc gcgcctatac ggccatcacc
      721 atggccgagg tcgccgagca agccggtctc gccaagggca cggtctatct ctacttcccc
      781 accaaagagg cgctgttcct ggcgttgcag cagcggatgc tggccgagtg gttcgccgag
      841 ctcgatgccg cgctggcgca gcttggaccg gcgtccgccg gcgccgtggc cgagaccatc
      901 tgccgcagcc tggccccgcg cccccacctg acgcgcttgc tggcgatctt gcacagcgtc
      961 ctggagcaga acatcgacta cgagaccgca gtcagcttta agcagatgct gctggcccac
     1021 atgcagcgga ccggggcgcg gctggagcgc tgcctgccct tcttgcgccc cggccagggt
     1081 gcccaggtgc tcctgcgcgc ccacgcgctg gtcatcggcc tgcaacacgt gagcgacccg
     1141 ccgcccctgg tgcgccaggt gctggagcaa cccgagctac aggtcttcca tatcgacttt
     1201 gccggcgagt tgtccgccac cctgcgcgcg ttgctgcgcg ggatggaatg cgtgcccggc
     1261 gacgacgcgg cccggcagta acaaaggaga acgcgaccca tggctaaaca ggcacaagcc
     1321 caccgcggcg agacggccct gatcaccggc gcatccagcg gattcggcgc ggagttcgcc
     1381 aagctgttcg cccgcgacgg ctacaacctg gtgctggtgg cccgcagcga gggcgccctg
     1441 aacaccctgg ccgatcagct acggaccagg tacggcgtgc aggtgcgcgt gctgcccaag
     1501 gacctggccc tgcccgccgc ccccgacgag atcttcgcca ccctgtgcga cgagaggatc
     1561 acggtcgatg tgctggtgaa caatgccggg ttcgccacct atggcccctt cgtcgagatc
     1621 ccgctccagg ccgacatgga cctgctcaag gtcaacatgc tcgcgctcac ccacctgacc
     1681 aagctgttcc tgcccggtat gctggcccgc cgccgaggca agatccttaa cgtggcctcc
     1741 actgccgcct tccaacccgg tccgctcatg gccgcctact atgccagcaa agcctacgtg
     1801 ctgttcctgt cggaggcgat tgccgaagag gtgcgcggct cgggcgtgac ggtgaccgcg
     1861 ctctgccccg gcccggcgcg taccggcttc cagcggcggg cgcagatgga agattcgcgg
     1921 ctggtcagcg gccggatcat ggacgcgcgc acggtggccg tggccggcta ccgggccctg
     1981 cagcagggcc acaccgtggt cattccgggg ctgcgtaacc ggatcttcgc gctggccccg
     2041 cgggtaagcc c
//
LOCUS       DASUYC010000670         2500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_980927,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000670 DASUYC010000000
VERSION     DASUYC010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980927"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1875
                     /locus_tag="VF276_10945"
     CDS             <1..1875
                     /locus_tag="VF276_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020601444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX6607419.1"
                     /translation="PAAERQIWLVREAGLGATARVPGAPDTWPGWEDSAVPPARLGDY
                     LRDLRRLFEQYDYDCAFYGHFGQGCVHVRIDFDLTSQPGIRKFRAFLQAAADLVLRYG
                     GSLSGEHGDGQARGELLPKMFGPELMHAFREFKAIWDPDWMMNPGKVVEAYRVDQNLR
                     LGVHYNPPQPITHFQYPDDGGSFARAVNRCVGVGNCRRLDGGTMCPSFMATREEAYTT
                     RGRAHLLFEMLRGEELGDGWRDPQVREALDLCLACKGCKGDCPVHVDMATYKAEFLAH
                     YYEGRLRPVTAYSIGLIHWWARLAARAPRLANFLTQTPGLRRVVQAAGGIAPQRRMPA
                     FAPQTFQAWFRKRPRRNQGRPPVILWADTFNNHFHPATAQAAVEVLEAAGFRVIVPRG
                     ALCCGRPLYDFGMLDAAKRLLAGCLDALHPALVRGVPVVGLEPSCVAVFRDEMLNLLP
                     NDEAAKRLHDQVFMLSEFLVKKVPHYQPPRLARKAVVHGHCHHKAIMKLDDEETILRG
                     MGLDYQILDSGCCGMAGAFGFERDHYDVSLRVGERVLLPAVRAAAPDTLIITDGFSCR
                     EQIAQTTDRRALHLAQVMQIALHDGPGGSPAPPPAAARPRQAAGRDSPSHAQEANNGL
                     "
     gene            1865..2194
                     /locus_tag="VF276_10950"
     CDS             1865..2194
                     /locus_tag="VF276_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607420.1"
                     /translation="MGFDQYHEPANELPAEVRTFARMILSLIEEAQAIDWYEQRLAVE
                     PDAEARAIMQNAQEEEFKHFGMDLEFLLRRKPKWRAATQAILFQPGDIVQHGKQGEKA
                     EDKTSGG"
BASE COUNT          406 a          825 c          879 g          390 t
ORIGIN      
        1 ccggcggcgg agcggcagat ctggctggtg cgtgaggcgg ggctgggcgc cacggcccgc
       61 gtgcccggcg cacccgacac ctggccgggc tgggaggact cggcggtgcc gcccgcccgg
      121 ctgggcgatt acctgcgcga cctgcgccgg ctgttcgagc agtacgacta cgattgcgcg
      181 ttctacggcc atttcggcca gggctgcgtc catgtgcgca tcgacttcga cctgaccagc
      241 cagccgggca ttcgcaagtt ccgcgcgttt ctccaggcag cggcggacct cgtgctgcgc
      301 tacggcgggt cgctgtcggg cgagcatggc gacgggcagg cgcgcggcga gttgctgccc
      361 aagatgttcg gccccgaact gatgcacgcc ttccgcgagt tcaaggccat ctgggacccc
      421 gattggatga tgaatccggg caaggtggtc gaggcgtacc gcgtggacca gaacctgcgc
      481 ctgggcgtcc actacaaccc gccccagccg atcacccatt tccagtaccc ggacgacggc
      541 ggcagcttcg cacgggcggt gaaccgctgc gtgggcgtgg gcaactgccg ccggctggac
      601 ggcgggacga tgtgtcccag cttcatggcg acgcgcgagg aagcctacac cacgcgcggg
      661 cgggcgcacc tgctgttcga gatgctgcgc ggcgaggagt tgggcgacgg ctggcgcgac
      721 ccgcaggtgc gtgaggcgct ggacctgtgc ctggcctgca agggctgcaa gggcgattgc
      781 cccgtccacg tcgatatggc gacctacaag gccgagttcc tggcccacta ctacgagggc
      841 cgcctgcgcc cggtgacggc ctactccatc ggcctgatcc actggtgggc gcggctggcg
      901 gcgcgggcgc cccggctggc gaacttcctg acccagacgc cgggcctgcg ccgcgtggtg
      961 caggcggcgg ggggcattgc cccgcagcgg cggatgccgg cgttcgcccc gcagacgttc
     1021 caggcgtggt tccgcaagcg cccgcggcgc aaccagggcc gcccgccggt catcctgtgg
     1081 gccgacacgt tcaacaacca cttccacccg gcgacggcgc aggccgcggt cgaagtactg
     1141 gaggccgcgg gcttccgggt gatcgtgccg cgcggggcgc tctgctgtgg ccggcccctg
     1201 tacgacttcg gcatgctcga cgcggccaag cggctgctgg ccgggtgcct cgacgcgctg
     1261 cacccggccc tggtgcgcgg ggtgccggtg gtgggcctgg agccgagttg tgtcgcggtc
     1321 ttccgcgacg agatgctcaa cctgctgccc aacgacgagg ccgcgaagcg gctgcacgac
     1381 caggtgttca tgctcagcga gttcctggtg aagaaggtgc cgcactacca gccaccgcgc
     1441 ctggcgcgca aggccgtcgt gcacggccac tgccaccaca aggcgattat gaagctggac
     1501 gacgaggaga cgatcctgcg cggcatgggc ctcgactacc agatcctcga ttccggctgc
     1561 tgcggcatgg ccggggcgtt cggcttcgag cgcgaccact acgatgtctc gctccgtgtg
     1621 ggcgagcggg tgctgctgcc cgcggtgcgc gcggcggccc ccgatacgct gatcatcacc
     1681 gacggcttta gctgtcgcga acagatcgcc cagacgacgg accgccgggc gctgcatctg
     1741 gcccaggtta tgcagatagc tttgcatgac gggccgggcg gctccccggc accacccccg
     1801 gcggcggcac gtccgcggca ggctgcgggc cgcgattccc cctcgcacgc acaggaggcc
     1861 aacaatgggc tttgaccagt accacgaacc ggccaatgag ttacccgcgg aggtgcgcac
     1921 cttcgcccgc atgattctct cgctgatcga ggaagcgcag gccatcgact ggtatgagca
     1981 gcgtctcgcc gtcgagccgg acgccgaggc gcgggcgatc atgcagaacg cgcaggagga
     2041 ggagtttaag cacttcggca tggacctgga gtttttgctg cgtcgcaagc ccaaatggcg
     2101 cgcggccacg caggcgatcc tgttccagcc gggggatatt gtgcagcacg gcaagcaggg
     2161 cgagaaagcg gaggataaaa cgagcggcgg ctagccgccc ccctcgtgtc attctcagcg
     2221 aagcgaagaa tctcgtctcg ttcgggtagg cgatgagact cttcgctgcg ctcagagtga
     2281 caagcccgcg agggccatgc tgaacgcagt gaagcatctc gccgggtctc aagcaagacg
     2341 cgactcttcg ctgcgctcag agtgacaaga ccgagcgggt catgctgaac gcagtgaagc
     2401 atctcgccgg gtctcaaacg agacgagact cttcgcttcg ctcagagtga caaggatgat
     2461 gagagagcct ttattggatt ggacaaatca taccaggata
//
LOCUS       DASUYC010000671         3148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_335878,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000671 DASUYC010000000
VERSION     DASUYC010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335878"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VF276_10955"
     CDS             complement(<1..322)
                     /locus_tag="VF276_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607421.1"
                     /translation="MRRRVGLGILGIVVLVICAGVMARFAAGQPQAPPIRSLDDTFPT
                     PLDPATAPAQPAGDTPLPLSTPTPRPVTPALATATAAPAPLVASATPGGPANPPGAVA
                     PADPA"
     gene            539..793
                     /gene="secG"
                     /locus_tag="VF276_10960"
     CDS             539..793
                     /gene="secG"
                     /locus_tag="VF276_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX6607422.1"
                     /translation="MDLRTALNIIQLIISILLIVLVLLQSKGSSIGSVFGGTDSSIYR
                     TRRGLEKRLYQFTIGVGIVFVLISLVSSVWGSAPAPAVGP"
     gene            complement(976..1926)
                     /locus_tag="VF276_10965"
     CDS             complement(976..1926)
                     /locus_tag="VF276_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607423.1"
                     /translation="MRTTRQLAGVSLLTLAALLFVSGFTSAAAVAQPLPQATGGQTIT
                     IAAGQSATLQVRGFCLDFGKLFPSQSNAPTGLAPDQVRAALNYAVSKGYDTSDPRQVQ
                     EAIWFLASGAWHRDDHALGQEIVDAAKNGNMPATPAGTSLIDALQANNLTATVSITPG
                     GGLTGSDAFYGDGTLTIKNNGTQDAQVYLPFGSVFPPANSAEQRLIGYALAAAQPAAT
                     TTAGATTTVEPSATTEATSTVEATTAPTETAMPTATTMPTETAAPTMAAAPTATPAAP
                     GLPRTGAGDAGLPGWSLLFGAALICLVAGLALLRRSAPPA"
     gene            complement(2215..2931)
                     /locus_tag="VF276_10970"
     CDS             complement(2215..2931)
                     /locus_tag="VF276_10970"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX6607424.1"
                     /translation="MSTIKASELKGRAVVSLSQAEKIGSVVSVILDPAASRVAGLTVR
                     TGILGGPKLLRASDIRSLGIDAVTIHDSSLLHDDSGEVPELKDLPTLDDMIEMKIISE
                     SGQLLGALGNVEIDPDQYQITRYELSGTLWDQLTHSNRTFDAAPGLRFGKQLLIVPDA
                     IGAALQRSSTPPDAGLEPLLPTAPPPGATPPAPPAESATFTPAPPPADTAAPPPAPAH
                     ARVILSDSEKDGKAPDAAPS"
BASE COUNT          473 a          970 c         1120 g          585 t
ORIGIN      
        1 gagcgggatc ggccggcgcg acggcgccgg gcgggttggc gggcccgcct ggcgtggcgg
       61 atgccacaag cggcgcgggc gcggcggttg ccgtagcgag ggccggcgtg acgggtcgcg
      121 gggtgggcgt gctgagcggc aggggtgtat cgcccgccgg ctgcgcaggg gcggtggccg
      181 gatcgagcgg ggtggggaag gtgtcgtcga gcgaacggat gggtggagct tgcggctgcc
      241 ccgccgcgaa ccgggccatc acacccgcac agatcaccag cacgacaatt cccagtatcc
      301 ccaacccgac tctacgacgc atccggtgga ttgccccttc tgttacatga tgccgcgtgc
      361 gggttcatta taagatgcgc ctgctccggc gacaacctgc gcttccggcg cggcgggcgt
      421 ttgcgcggcc cgtgaaaaca tggtataatc ggcaggaccc tcttccgcga gggctccgcg
      481 acctgggttg cgttccgccg gatctaccac ggctcgatat gtatcaagga gtgtacttgt
      541 ggacctgcgc actgcgctga atattatcca gttgattatc tcgatcctct tgatcgtcct
      601 ggtgctgttg cagtccaaag gctccagcat cggatcggtg tttggcggga ccgacagcag
      661 catctaccgc acccgccgcg ggctggaaaa gcggctctac cagttcacga ttggggtcgg
      721 catcgtgttt gtgctcatct cgctggtgag cagcgtgtgg ggcagcgccc cggcacccgc
      781 cgtcggcccg taaagccccg cgcctttccg ccgcctacat aaagatcaac ccgccgggtg
      841 tcttaccggc gggttgatct tttgccgacc gctggccgcc ttccggtcgg gtagcgcgct
      901 gtaaacagag cgccgctgcg tccaggttgg gacgcagcgg cctttacatc ttgctattcg
      961 gctgcgacgt gcggtttatg ccggcggggc gctccggcgc aggagggcca ggccggccac
     1021 caggcagatc agcgcagcac cgaacagcaa tgaccagccg ggcaaccccg cgtcacctgc
     1081 gcccgtgcgc ggcaggccgg gggcggccgg cgtcgccgtg ggcgcggcgg ccatcgtcgg
     1141 tgccgccgtc tcggtgggca tcgtcgttgc cgtgggcatg gccgtctcgg tcggcgcagt
     1201 ggtcgcctcc accgtactgg tggcctccgt ggtggcggac ggctcgaccg tggtggtggc
     1261 cccggcggtc gtggtggccg cgggctgggc cgccgccagc gcgtagccga tcagccgctg
     1321 ctcggcgctg ttggcgggcg ggaagacgct gccgaagggc aggtagacct gggcatcttg
     1381 cgtgccgttg ttcttgatcg tcagcgtgcc gtcgccgtag aaggcatcgc tgccggtcag
     1441 gccgccgccc ggcgtgatgc tgactgtcgc cgtcaggtta ttggcttgca gcgcgtcgat
     1501 cagggacgtg ccggcggggg tcgcgggcat attgccgttc ttggccgcgt cgacgatttc
     1561 ctgccccagg gcatgatcgt cgcgatgcca cgcgccactc gcgaggaacc agatcgcttc
     1621 ctgtacctgg cgggggtcgc tggtgtcgta gcccttgctg acggcatagt tcagcgcggc
     1681 acgcacttgg tcgggtgcca ggccggtggg cgcattgctc tggctgggga agagcttgcc
     1741 gaagtccagg caaaagccgc gcacttgcag cgtggcgctc tgcccggcgg cgatggtgat
     1801 cgtttgcccg cccgtggcct ggggtaaggg ttgggccacg gcggcggcac tggtaaaacc
     1861 ggatacaaac aacagcgccg ccagggtaag caagcttact ccggccaatt gtcgagtcgt
     1921 gcgcatggcg tagggtctcc ttttactcgt cgatgtaggt ctgtaacgtt attgctgccg
     1981 gtcgctggga ccggggagca aggccgcgca gatcgggccg cgtacaagcc gcagccgtga
     2041 tctttgcaca gggacccaga agcaatgatc atgccagata cggagcgccc cggcacggcc
     2101 cttgctcaga gattgccggg gcaatgcgaa agcccttcct gattgaaggg cttccgagtg
     2161 tagctatcga aattggatat atatagaaag cggcaatgca cccgccgccc ggattcagga
     2221 tggtgccgcg tccggggcct tgccgtcctt ctcgctatcg ctgaggatca cccgcgcgtg
     2281 ggccggggcg ggcggcggcg cggcggtgtc tgccgggggc ggcgcgggcg taaaggtcgc
     2341 gctctcggcg ggcggcgcgg gcggcgtcgc gccgggcggc ggggcggtcg ggagcagggg
     2401 ttcgagccca gcatcgggcg gggtgctcga ccgctgcaag gccgcgccga tcgcgtccgg
     2461 aacgatgagc aattgcttgc cgaagcgcag acccggtgcc gcgtcgaaag tgcggttgct
     2521 atgcgtcaac tggtcccaca acgtgccgct cagttcatag cgcgtaatct ggtattgatc
     2581 cggatcgatt tcgacattgc ccagggcgcc cagcaactgg ccgctctcgc tgatgatctt
     2641 catttcgatc atgtcgtcca gcgtgggcag gtctttcagt tccgggactt cgccggagtc
     2701 gtcatgcagc aggctgctgt cgtgaatggt cacggcgtcg atgcccaggc tgcggatatc
     2761 gctggcccgc aggagcttgg gaccgcccag aatgccggtg cggaccgtca gcccggccac
     2821 ccggctcgcc gcgggatcca ggatcacgct gacgacgctg ccgatttttt cggcctggct
     2881 caagctgacc accgcgcggc ctttgagttc gctcgctttg atcgtgctca cgttgtcctc
     2941 ctgctggctg gggggatcct gccgcggcgc cctgtggcgc ggatgcacgg ccagtataat
     3001 acaagtcgcg tggttgttca ataaccctgg ggatgatata atccgcattg cgtgagattg
     3061 caaaggaaca aggcttggat cagcagcagc agcagcgaaa cgctgaagac gaagatttga
     3121 ttgcgcgcat ggccgctcgc gatcccac
//
LOCUS       DASUYC010000672         2354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_685717,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000672 DASUYC010000000
VERSION     DASUYC010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685717"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..109)
                     /locus_tag="VF276_10975"
     CDS             complement(<1..109)
                     /locus_tag="VF276_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008212317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX6607425.1"
                     /translation="MKVTWSGVLPAITTPFNADYSVDHGFFAEHIRWMAD"
     gene            complement(106..1377)
                     /locus_tag="VF276_10980"
     CDS             complement(106..1377)
                     /locus_tag="VF276_10980"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HEX6607426.1"
                     /translation="MKRLSFDVLVVGAGPAGMAAALAAAESGARVGLVDDNPAPGGQI
                     WRGAGQAAAAPGAPWLARLQASAITVLPGTRIVAPLDDRRLLAESATGAMLLGYGRLI
                     LATGARELFLPFPGWTLPGVLGAGGLQALVKGGLPIAGKRVVVAGSGPLLPAVAAYLK
                     RHGARVLLIAEQTPWAKLLPFGLRLLAERDKLRQGAGFGWTLAGTPFRAGCWVMEAHG
                     AERLEAVSVRRGARTERIACDYLACGFGLLPNVELAAALGCAIRGGAVRVDRRQQTSR
                     AGVYCAGEATGVGGVDLALVEGRIAGLAATDRWDAAQRDLVERARARRFAAALARTFA
                     PRPELRALPRADTLVCRCEDVPYGELAAQPGWRAAKLHTRCGMGPCQGRVCGGATEFL
                     FGWTQDSVRPPVAPASIASLAQITTTEEVAQ"
     gene            complement(1374..1625)
                     /locus_tag="VF276_10985"
     CDS             complement(1374..1625)
                     /locus_tag="VF276_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX6607427.1"
                     /translation="MPDPPLIRLTVNGHEVRVPPGTVVAAAIAVAGVTSYRRSVRGEA
                     RAPLCGMGICYECRVAIDGQAQRLSCQTVCAEGMQVVTE"
     gene            complement(1618..>2354)
                     /locus_tag="VF276_10990"
     CDS             complement(1618..>2354)
                     /locus_tag="VF276_10990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012733234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX6607428.1"
                     /translation="RAGLAGGLLVPGDSVVYPPAAAEALLARAAQQGARVIAGTAIRL
                     VPGGVELADGSVIHSEAIVIANGARAVELAPELPISPKKGHLAITDRYPGVVRHQLVE
                     LGYVKSAHATHGDSVAFNVQPRATGQLLIGSSRQPDVATREVDYAMLARMLARAVAYL
                     PELAGLSCIRVWTGLRAATPDGLPLIGPHPAWPGVWIAGGHEGLGITTSLATGQLLAA
                     QMLGRTPPIPVEPYLPARVLQEAAHA"
BASE COUNT          332 a          938 c          804 g          280 t
ORIGIN      
        1 cgtccgccat ccagcggata tgctcggcaa aaaaaccgtg gtccacgctg taatcggcgt
       61 tgaacggcgt ggtaattgcg ggcaacaccc cgctccaggt cactttcatt gggcaacctc
      121 ctcggttgtg gtaatttgcg caagactcgc tatcgacgcg ggggccaccg gcgggcgcac
      181 cgagtcctgg gtccagccga acaggaactc cgtggccccg ccgcacaccc gcccctggca
      241 cggccccatg ccgcagcgcg tgtgcagctt cgcggcgcgc cagcctggct gggccgctag
      301 ctcgccgtag ggcacatcct cacagcggca gacgagcgta tcggcccgcg gcagcgcccg
      361 cagttcgggg cgcggggcga aagtgcgcgc cagcgccgcc gcgaagcgcc gggcccgcgc
      421 ccgttccacc agatcgcgct gcgccgcgtc ccaccggtct gtcgcggcca gcccggcgat
      481 gcgaccctcg accagggcca ggtccacgcc gcccacgccg gtcgcctccc cggcacagta
      541 caccccggcg cggctggtct gctgccggcg gtccacccgc accgccccgc cgcggatcgc
      601 gcagcccagc gcggcggcta gttcgacgtt gggcagcagg ccgaagccgc aggccaggta
      661 gtcgcaggca atccgctcgg tgcgcgcccc ccggcgcacg ctcaccgctt ccagccgctc
      721 cgcgccatgc gcctccatca cccagcaacc ggcgcgaaac ggcgtgccgg ccagcgtcca
      781 gccgaagccc gcgccctggc gcagtttgtc gcgctcagcg agcaggcgca ggccgaaggg
      841 cagcagcttc gcccacggcg tctgttcggc gatcagcagc acgcgggcgc cgtggcgctt
      901 gaggtaggcg gccacggccg gcagcagcgg cccgctcccg gccaccacca cccgtttgcc
      961 ggcgatgggc agcccgccct tgaccagcgc ctgcaacccg cccgccccca gcacgccggg
     1021 caaggtccag ccggggaaag gcaggaacaa ctcgcgagcg cccgtcgcca ggatcagccg
     1081 cccgtagccg agcagcatgg cgccggtagc gctctccgcc agcaggcgcc ggtcgtccag
     1141 cggggcgacg atgcgcgtgc cgggcagcac cgtgatcgcg ctggcttgca gccgggccag
     1201 ccagggcgcg cccggcgcag cggcggcctg gcccgcgccg cgccagatct ggccgccggg
     1261 cgccgggttg tcgtccacca ggcccacccg cgcgccgctc tcggcggcgg ccagcgcggc
     1321 ggccatcccc gccggccccg ccccaacaac cagcacgtca aagctcaggc gcttcattcg
     1381 gttaccacct gcatgccctc ggcgcagacc gtctggcagc ttaaccgctg cgcctgccca
     1441 tcgatggcga cccggcactc gtaacagatg cccatgccgc acagcggcgc ccgcgcctcg
     1501 ccgcgcaccg agcggcggta actggtcact cctgccaccg cgatggcggc ggccaccacc
     1561 gtgcccggcg gcacccgcac ctcgtgcccg ttcaccgtga ggcggatcaa aggcggatca
     1621 ggcatgggcg gcctcctgca acacgcgggc gggcagatac ggctccaccg ggatcggcgg
     1681 cgtgcggccc agcatctgcg cggccagcag ttggccggta gcgagcgacg tggtgatgcc
     1741 cagcccctcg tgcccgcccg cgatccacac gccgggccac gcggggtgcg ggccgatcag
     1801 cggcagcccg tcgggcgttg cggcgcgcag ccccgtccag acgcggatac acgacagccc
     1861 ggccagttcg ggcaggtagg ccaccgcgcg ggccagcatc cgcgccagca tcgcgtagtc
     1921 cacctcgcgc gtcgccacgt cgggctggcg cgacgagccg atcagcaact ggcccgtggc
     1981 ccgcggctgc acgttgaagg ccaccgagtc gccgtgcgtg gcgtgcgcgc tcttcacgta
     2041 gcccagttcg accagttggt ggcggacaac gcccggatag cggtcggtga tcgccaggtg
     2101 gcccttcttc gggctgatcg gcagttcggg cgccaactcg acggcgcgcg ccccgttggc
     2161 gatgacgata gcctcgctgt ggataacgct gccgtccgcc agttccacgc cgcccggcac
     2221 caggcggatc gccgtgcccg cgataactcg cgcgccctgc tgcgcggccc gcgccagcag
     2281 cgcctcggcg gcggcgggcg gatagacgac gctgtcgccc ggcactagca gcccgcccgc
     2341 cagcccggcg cgca
//
LOCUS       DASUYC010000673         2033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1768022, whole genome shotgun sequence.
ACCESSION   DASUYC010000673 DASUYC010000000
VERSION     DASUYC010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1768022"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VF276_10995"
     CDS             <1..297
                     /locus_tag="VF276_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012544396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX6607429.1"
                     /translation="YLETPSSWSLDDKRMNFHFAVEMCREIKGGKLGRLYKAASFQDR
                     TPHFWGSVAAVGGPQAWRLWGLPGCAKGEPLQSCHVGHGAAPVLVHGLPVRRGG"
     gene            297..1685
                     /locus_tag="VF276_11000"
     CDS             297..1685
                     /locus_tag="VF276_11000"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEX6607430.1"
                     /translation="MDTLPAGPDRDAMEAALAASPAEQTEIVLLRDVTAVTRYANSEI
                     HQNVYQDNSRAAVRVAVGGATARVYTNDTSRAGLTRATADATALARLQAPNPRFHSLP
                     GPADGSTPEREAPPSFFTATAELSPEARAAAVGQVIDLARAEGYQAYGTYRSSRSSLS
                     VANSLGVRAHAAFTTAYLKALVESGAGTGYADALSRDAATIDPAAVGAVAVAKCRLNH
                     DQREIAPGEYAAVFEPNAVADMVRFPVLFGLGAREYQDGQSFLSGRMGEPVSGPNWTL
                     WEDPRDPRAMPLAIDAEGMPAVRVPLIDQGVGVGVVYDSETAGLEPGRRSTGHAADPF
                     DSIEQTNPLPGHLVMPTGTETSLELARGLQRGILVTRFHYTGCPDPKRVIATGTTRDG
                     TFLVEHGEIVAALKNLRFEMSVLDLMAGIEAWGAGKCCQDWWAANGMGGTTYYLPSLR
                     FARARFIGVTTF"
     gene            1777..>2033
                     /locus_tag="VF276_11005"
     CDS             1777..>2033
                     /locus_tag="VF276_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607431.1"
                     /translation="MQKWEYAWIKQMRGYGGTLFGGTADKWNFDTGTMSSNIMKYYAE
                     KRNIFDIANMMGDDGWELINMVPQSGIGGTASSGFTSDLVWV"
BASE COUNT          348 a          673 c          706 g          306 t
ORIGIN      
        1 tacctggaga cgccgagttc gtggagcctg gacgacaagc gcatgaactt ccacttcgcg
       61 gtggagatgt gccgcgagat caagggcggc aagctgggcc ggctctataa ggccgcttcg
      121 ttccaggacc gcacgccgca cttctggggc agcgtcgccg ccgtgggcgg gccgcaggcg
      181 tggcgcctct gggggctgcc gggctgcgcg aagggcgagc cgctgcaaag ctgccatgtg
      241 ggccacgggg ccgcgccggt gctggtacac gggctgcccg tgcgccgggg gggatgatgg
      301 atacactacc tgccgggccg gaccgcgacg cgatggaagc ggcgctggcc gcctcgcccg
      361 ccgagcagac ggagatcgtg ctgctgcgcg acgtgaccgc cgtgacccgc tacgccaact
      421 cggagatcca ccagaacgtc taccaggaca acagccgcgc cgccgtgcgc gtggcggtgg
      481 gcggggccac ggcccgcgtc tacactaacg acaccagccg cgccgggttg acgcgcgcca
      541 ccgcggacgc gacggccctg gcgcgcttgc aggcgcccaa cccgcgcttc cactcgctgc
      601 ccggccccgc cgacggcagc acgccggagc gcgaggcgcc gccctcgttc ttcaccgcaa
      661 ccgccgagct atcgccggag gcgcgggccg ccgccgtggg ccaggtgatc gacctggcgc
      721 gggcggaggg ctaccaagcc tacggcacct accgcagcag ccgcagcagc ctgagcgtcg
      781 ccaactcgct gggcgtccgc gcccacgccg ccttcacgac cgcctacctg aaggcgctgg
      841 tcgagagcgg agcgggcacc ggctacgccg acgcgctgag ccgtgacgcc gcgacgattg
      901 acccggcggc ggtgggcgcg gtggccgtcg ccaagtgccg cctgaaccac gaccagcgcg
      961 agatcgcgcc cggcgagtac gccgccgtgt ttgagccgaa cgccgtggcc gatatggtgc
     1021 gcttcccggt gctgttcggc ctgggcgcgc gcgagtacca ggacggccag tcgttcctgt
     1081 cggggcgcat gggcgagccg gtcagcgggc cgaactggac gctgtgggaa gacccgcgcg
     1141 acccccgcgc tatgccgctg gcgatcgacg ccgagggcat gccggcggtg cgcgtgccgc
     1201 tgatcgacca gggcgtgggc gtgggcgtgg tctacgacag cgagacggcc gggctggagc
     1261 cgggccgccg ctcgaccggc cacgccgccg acccgttcga ttccatcgag cagaccaacc
     1321 cgctacccgg ccacctcgtg atgcccaccg gcacggagac cagcctggag ctggcgcgcg
     1381 gcctgcaacg cggcatcctg gtgacccgct tccactacac cggctgcccc gaccccaagc
     1441 gggtcatcgc caccggcacc acgcgcgacg gcaccttcct ggtggagcac ggcgagatcg
     1501 tggccgcgct caagaacctg cgcttcgaga tgagcgtgct cgacctgatg gccgggatcg
     1561 aggcgtgggg cgcgggcaag tgctgccagg actggtgggc ggccaacggc atgggcggca
     1621 ccacctacta cctgcccagc ctccgcttcg cccgcgcccg cttcatcggc gtgacgacgt
     1681 tttagctatc cagttttcgg ataacttata cactgatcga gtatcgcaac tgctaaccgt
     1741 gagagtgatg agtactaata accctggagg tgcgctatgc agaagtggga gtatgcatgg
     1801 atcaagcaga tgcgtggcta tggtggaact cttttcggag gaactgcgga caaatggaac
     1861 tttgatacag gtactatgtc ttccaacata atgaagtatt atgccgagaa aaggaacatt
     1921 ttcgatatag cgaatatgat gggagacgat ggatgggagc tgataaacat ggttcctcaa
     1981 tccggaatag gtggtacagc atcatcaggt tttacgagtg atttagtttg ggt
//
LOCUS       DASUYC010000674         2926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1212678, whole genome shotgun sequence.
ACCESSION   DASUYC010000674 DASUYC010000000
VERSION     DASUYC010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1212678"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1180)
                     /locus_tag="VF276_11010"
     CDS             complement(<1..1180)
                     /locus_tag="VF276_11010"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HEX6607432.1"
                     /translation="MIAAGIGAGLLLMVGAICFLLYLAWPLLESGGRRPAVSPDEAIS
                     QAAQFAILAFYGLVLGGALLRQVIRGSRGAPSARFHPATVAGVLAVVGFPVALLAGIV
                     ALQLPHRVAGYLFPLAALLAVLTPPVLVLNLMGRAGWRRAGEPALAAAPSRPGGLPGR
                     TSREDPFAADLVRRVVGPPTWRRILGALAWGMGGATAISLVLEVVGAIAALLLVMGFA
                     LTGGPGQLTQWQNLLTGDAAAAGCDTLRSVFGNPLVVGGLVLAVGVFVPLIEEPAKSL
                     GVLWLRGRLSAPRDALLYGAVAGIGFGMIESAVYNVGDLTTWGGTATLRLGTTFVHAL
                     ATGLFGVAWYYRLQRRDRGRFWRYALASVGVHGLWNASTVGIIAVGATRSCTADPVGL
                     V"
     gene            complement(1215..2534)
                     /locus_tag="VF276_11015"
     CDS             complement(1215..2534)
                     /locus_tag="VF276_11015"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX6607433.1"
                     /translation="MNTNAFRLLLLTVALYIAALTLGLRLLYYLSYVLSAVLICSWFW
                     ARLNLSALEIRREARRQQAQVGDVFEETFLVRNRSIIPKLWVEVRDHSTLPGHQAAAV
                     ISIAGDKSKRWRVRTLTRRRGLYQLGPLTAISGDPFGLFRSWRTVPEMGELLVYPATV
                     DLSQFGLPSGEMPGGTAVRGRSMSTTPNASGIREYLAGDSLNRIHWGATARYQRLMVK
                     EFELDPTSNIWIVLDLDRAVHLSASPTTGGAPRAIGDTRMLHVRGAVEPTSDADAGRP
                     IYLDPETEEYAVTIAASLAAHFIQQGRDIGLLATAHHPVIIDPERGARQLVKLLRTLA
                     VVRAEDPLPLAQMLVGQSTQFGRGDSLVVITPSVSETWVAALNEITTRGARATAILIE
                     PHTFGSSQSSIMTVGALAAVDIPSYLVKRDDTLELALSREMAHGHLR"
     gene            complement(2531..>2926)
                     /locus_tag="VF276_11020"
     CDS             complement(2531..>2926)
                     /locus_tag="VF276_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX6607434.1"
                     /translation="DTLAQAVDARELIEAQTAIRTVYVDRLIKEYILGIVEATRKHDD
                     VYLGASPRGSLALYRTAQAHAAVDGRDFVIPDDVKNLAAATLAHRIILRPAARIKNTD
                     ARTIIDEILRRLPVPGVRAGAPKSSAHPA"
BASE COUNT          507 a         1019 c          987 g          413 t
ORIGIN      
        1 tgaccagtcc cacgggatcg gcggtgcaac tgcgcgtcgc gcctactgcg attataccaa
       61 ccgtgctggc gttccacaat ccatgtacgc cgaccgaggc cagcgcgtaa cgccagaacc
      121 ggccgcgatc ccggcgctgc aagcggtaat accaggccac gccaaacaaa ccggtggcga
      181 gcgcatgtac gaaggtggtg cccagccgca aggtcgccgt gcccccccag gtagtcaggt
      241 cccctacatt atatacggcg ctttcaatca tgccaaagcc gatccccgcc accgcgccgt
      301 acagcagggc gtcgcgcggc gcgctgagcc gtccgcgcag ccagagcacg ccgagcgact
      361 tggccggctc ttcgatcaac ggcacgaaga cgcccaccgc cagaactaac ccgcccacga
      421 cgagcgggtt cccgaagacg ctgcgcaacg tgtcgcagcc cgctgccgcc gcatcgcccg
      481 tgagcaggtt ctgccactgg gtgagttgcc ccggcccgcc ggtcagggca aagcccatga
      541 cgagcagcag cgcggcgatc gcccccacca cctccagcac cagcgagatg gcggtggcgc
      601 cgcccatgcc ccaggccagc gcgcccagga tgcgccgcca ggtgggcgga cccacgaccc
      661 ggcgcaccag gtcggcggcg aacgggtctt cgcggctggt gcgccccggc agtccgccgg
      721 gccgcgacgg ggccgctgca agcgcaggct cgccggcccg ccgccagccc gcccggccca
      781 tcaggttcag caccaacaca ggcggggtca gcaccgccag cagcgccgcc agcgggaaga
      841 ggtagcccgc cacccggtgc ggcaactgca aggccacgat gccggccagc agcgccaccg
      901 gaaagcccac caccgccagc accccggcca cagtggccgg atgaaaccgg gccgacggtg
      961 cgccgcgcga tccccggatg acctgccgca gcagggcgcc gcccagcacc agtccataga
     1021 acgccaggat ggcgaattgc gccgcctggc tgatcgcttc gtcgggactg actgccggcc
     1081 tacgcccccc gctttccagc aacggccagg ccaggtagag caagaaacag atcgccccga
     1141 ccatcagcag caggccggcg ccgatgcctg ccgcgatcaa taggccgctt gctcgccgtg
     1201 gcggtaccgc cagactagcg gaggtgacca tgcgccattt cgcgcgacag cgccaattcg
     1261 agcgtgtcgt cgcgcttgac cagatagctc gggatatcga ccgccgccag cgcgccgacc
     1321 gtcatgatcg acgattgact gctgccgaag gtgtgcggct cgatcaagat cgcggtcgcg
     1381 cgcgcgccgc gggtcgtgat ctcgttcaac gccgccaccc aggtctccga gacggaaggg
     1441 gtgataacga ccaggctgtc gccccttccg aactgggtgg attggccgac cagcatctgg
     1501 gccagcggca agggatcttc cgcgcgcacc acggccagcg tgcgcagcag tttcaccaac
     1561 tggcgtgcgc cgcgctctgg gtcgatgatc accgggtggt gtgccgtcgc cagcagcccg
     1621 atatcgcggc cctgctgaat aaagtgggcg gccagcgagg cggcgatggt gacggcgtac
     1681 tcctcggtct cggggtcgag ataaatcggg cgcccggcgt cggcgtcgct ggtgggttcg
     1741 accgcgccgc gcacatgcag catgcgcgta tccccgatag cgcgcggggc gccgcccgtg
     1801 gtaggtgacg cgctcaaatg cacagcgcga tccaggtcga gcacgatcca gatgttgctg
     1861 gtcgggtcga gttcaaactc cttcaccatc aaccgctggt agcgagcggt ggccccccag
     1921 tggatgcggt tcagcgagtc cccggccagg tattcgcgga tgccgctggc gttgggcgtc
     1981 gtagacatcg agcggccccg cacggccgtg ccgccgggca tttcgccgga cggcagcccg
     2041 aactggctga gatccaccgt ggccgggtag accagcagct cgcccatctc gggcacagtg
     2101 cgccagctcc gaaacaggcc gaagggatcg cccgaaatgg ccgtcagcgg gcccagttgg
     2161 tagaggccgc gacgccgggt gagcgtgcgc acccgccagc gtttcgactt gtcgccggcg
     2221 atgctgatga ccgccgcggc ctggtggccg ggcagagtgg aatgatcgcg cacttccacc
     2281 cagagcttgg ggatgatgct gcggttgcgc accaggaagg tttcttcaaa gacatcgccg
     2341 acctgcgcct gctggcgccg ggcctcgcgg cggatctcca gggcgctcag gttgagccgc
     2401 gcccagaacc aggagcagat gaggacggcg ctcagcacgt agctgaggta ataaagcagc
     2461 cgcagcccca gcgtcagcgc cgcaatgtag agcgcgaccg tcagcaggag cagccggaat
     2521 gcattggtgt tcatgccggg tgcgcgctac ttttcggcgc ccccgcccgc acgccgggca
     2581 caggcagccg ccggaggatc tcgtcgataa tagtgcgcgc atccgtattc ttgatccgcg
     2641 ccgccgggcg caagatgatg cggtgggcca gggtggccgc cgccaggttt ttcacatcgt
     2701 cggggatgac aaagtcgcgg ccgtccacgg cggcgtgggc ttgcgcggtg cgatacagcg
     2761 cgagcgagcc gcgcgggctg gcgccgagat agacgtcgtc gtgcttgcgg gtcgcttcga
     2821 cgatgccgag gatatattcc ttgatcaatc gatccacgta cacggtgcgg atagcggtct
     2881 gggcttcgat cagttcgcgg gcatccacgg cctgggccag ggtgtc
//
LOCUS       DASUYC010000675         6064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_507475,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000675 DASUYC010000000
VERSION     DASUYC010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6064
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507475"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1784)
                     /locus_tag="VF276_11025"
     CDS             complement(<1..1784)
                     /locus_tag="VF276_11025"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX6607435.1"
                     /translation="MHIAFLNPQGNFDPKDSHWTAHPDFGGQLVYVKELALATGALGH
                     RVDIVTRQIVDPAWPEFAAPVDGYPGHPNVRILRIPCGPPTFLPKEELWPFLGTEFVP
                     RLLDLYRREGHLPDAATAHYGDGGLCAAILEEQAGIPFSFTAHSLGAQKLDKLGANRG
                     NLALLNERYHFTQRLMGERLAMNRSVVNITSTSQERYSQYTHSAYRGAIDPSDDSRFA
                     VVPPGVAMSIFDRQSRYADEAGVHAHVQAALERDLRPERVGLPCIVASSRLDPKKNHL
                     GLLEAFAGSESLRRIANLVIITADLEQPLEGYEKAGPGERAVLDTLMTVIDRSQLRGQ
                     VAMLALHGQGELAAGYRFFAGLHSVFALTALYEPFGLAPLEAMAAGLPAVVTKFGGPS
                     ESLRSETDDYGILVDPNDLAEVSAGLFALVSNLTTWQEYATKGYERVLARYTWQRTAE
                     GYLAALRGRNRAVDEPEKEIRATSGTGSHQAVEAVAPHSGPAGAKRDNRLPIPGFFFD
                     PQGSHEDLLALLDDLYLKLDVLAVGETLVDFLSHKRVRSLRTADEFTRYLGGQPANVA
                     VYVAKLGGRSAVISKVGKGYVADFLEEQL"
     gene            complement(1921..3030)
                     /locus_tag="VF276_11030"
     CDS             complement(1921..3030)
                     /locus_tag="VF276_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607436.1"
                     /translation="MRAMIDEASRSITNLPEQVAGLDADGRARFADLFAVTRSGGSLQ
                     PPPQMRAWIERSFGSVEAVQAQTIIKTLNRWTLEGTLFNDLRARRPVARQAPGANADA
                     ELAGGGDDPFCHPLTGTPADTFGRIEGTHTITASNVAKYDALHAVILFKEHNPLTWSG
                     AQVADAFDTAARWIAAAAAARPEAVYPFVMWNCRPRSGASQVHGHMQATLGEGGAYGR
                     VELWRRAAEGYRARHGRDYFADFYAAHAGLGLTGRHGDVRWMAHLTPVKEKELVLLAP
                     AFDGALAAAVADLLRLLIDTLGVRAFNVACYLPPLAPVAEDWTDFPVVVRMVDRGDPG
                     SATSDIGAMELFAQPVVAFDPWKLAGAVREAFPAA"
     gene            3380..4726
                     /locus_tag="VF276_11035"
     CDS             3380..4726
                     /locus_tag="VF276_11035"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX6607437.1"
                     /translation="MGELRSASVTLAEIMAYIKQTRQTGRLTVRIQGPAGTAEGPAAL
                     IFETGHLVDARNATESGDDLVYRLLGQREATYSFDRLSADQIPQERTITRVQELLMLA
                     AIGILSEEDAGPSDSGRTEAIEAATQPSPPPPPAPAPVAKEAPLPPPAPKGASTDVPP
                     RRRPRSFRRRNMIPLPPGQPAYTGMAPPRDLMSLVDRLEHDLFSGYVTWFSEGAEGLL
                     LLYQGQVIDAFWAEVQTAATYAERPAIRQFAAAVRSNGHNPIEVYELDPDFVWSYSSL
                     AYGAGNPSRQGMDHVQLPQLLVHLAHVEHTGCVKVVTGNQAAYVFLCGGRPLGEYRAL
                     PHSLQTAPGRAMTLAGMPGCLVDVYTSPTPAELLALNAATWPVERVIEELRRTARDVL
                     GNKAGQVLTLITNAGSDPGALHAACARAKQVTRVFIGPEKHAELSRRLDRLLAHLQ"
     gene            4750..>6064
                     /locus_tag="VF276_11040"
     CDS             4750..>6064
                     /locus_tag="VF276_11040"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX6607438.1"
                     /translation="MGTVVTPVTQSQAAPSDPHLMAYFRALPACETPDAALRLTLAGA
                     LAMTGAASGAALAAVSPAKPLTAGSPDGDLAAQIQAAWAAAAGAPAFFAPADPAPPES
                     AVDAPRYVPLAAGGRLYGLLGLRGGPALDPATADALDVMAAATGRALQMSINLDELRG
                     LYEVATALNSTLELPVLLKVIVKRTTAALRSEACTLMLLDERTQELVFEIPVGRAENA
                     LRQLRMPLSQGICGWVARNGCPAIVNDPRADERFHTSVDQSSGFVTRNALAVPLILRG
                     RVGGVVEVLNKLGNEPYSAHDVELLTTLAGQAAVALDNAQLYTSLQIEHERLLAAEEQ
                     VRKELARDLHDGPAQRLAAICMEVEILRKLLVKDPSRLPEELDALETLARKANREVRT
                     MQFQLRPVMLETRGLRAAVEYYVNQLRETERVAFTVEVAGCEDRLP"
BASE COUNT          951 a         2173 c         1997 g          943 t
ORIGIN      
        1 agttgctcct ccagaaagtc ggcgacgtag cccttgccca ccttggagat caccgccgac
       61 cggccgccga gcttcgccac atagacggcc acgttggccg gctgcccgcc caggtagcgc
      121 gtgaactcgt cggcggtgcg caacgagcgc acccgcttgt gcgacagaaa gtccactagc
      181 gtctcgccga cggccagcac gtcgagcttc aggtagagat catccagcag cgcgagcagg
      241 tcctcgtgtg atccctgcgg atcgaagaag aagccgggga tcggcagccg gttgtctcgc
      301 ttcgcgcccg cggggccgct gtggggcgcc accgcctcca cagcctggtg gctacctgtg
      361 cccgacgtgg cccggatctc cttttccggt tcgtccacgg cgcggttgcg cccgcgcagc
      421 gcggccaggt agccctcggc ggtgcgctgc caggtgtagc gggccagcac ccgctcgtag
      481 cccttggtgg cgtattcctg ccaggtggtc agattgctta ccagcgcgaa caggccggcg
      541 ctgacctcgg ccaggtcgtt ggggtccacc aggatgccgt aatcgtcggt ctccgagcgc
      601 aggctttcgc tggggccgcc gaacttcgtg accacggcgg gcagccctgc cgccatcgcc
      661 tccagcgggg ccaggccgaa cggctcgtag agcgcggtca gcgcgaagac cgaatggagt
      721 ccggcgaaga agcgataacc cgctgccagt tcgccctggc cgtgcagcgc cagcatggcg
      781 acctgcccgc gcagttgcga ccggtcgatc acggtcatca gggtgtcgag cacggcccgc
      841 tcgcccggcc cggctttctc atagccttcc agcggctgct ccaggtccgc cgtgatgatg
      901 accaggttgg cgatccggcg cagcgactcg ctgccggcga acgcctccag caagcccagg
      961 tggtttttct tggggtcgag ccggctggac gccacgatgc atggcagccc cacccgctcc
     1021 gggcgcagat cgcgttcgag cgccgcctgc acatgggcgt gcacaccggc ttcgtcggcg
     1081 tagcggctct gccggtcgaa gatgctcatc gccacgcccg gcggcaccac ggcgaagcgg
     1141 ctgtcgtcgc tggggtcgat ggccccgcgg taggcgctgt gggtgtactg gctatagcgc
     1201 tcctgcgagg tgctggtgat gttgaccacc gagcggttca tcgccagccg ctcgcccatg
     1261 agccgttgcg tgaaatggta gcgctcgttc agcagcgcca ggttgccccg gttggcgccc
     1321 agtttgtcga gcttctgggc gcccagcgag tgcgcggtga agctgaaggg gatgcccgcc
     1381 tgctcctcca ggatggcggc gcagaggccg ccgtcgccgt agtgcgccgt cgccgcgtcg
     1441 ggcaggtggc cctcgcggcg gtagagatcg agcaggcgcg gcacgaattc ggtgcccagg
     1501 aaaggccaga gttcttcctt cggcaggaac gtgggcggtc cgcaggggat gcgcaggatg
     1561 cgcacgttag ggtggccggg gtagccgtcc accggcgcgg caaactccgg ccaggccggg
     1621 tcgacaatct gacgggtcac gatgtccacg cgatggccga gcgccccggt cgccagcgcg
     1681 agttctttca cgtagacaag ctggcccccg aagtcggggt gggccgtcca gtggctgtcc
     1741 ttgggatcga aattgccctg cgggttgaga aacgcgatat gcatggtcgc atcctcctgc
     1801 tcagtagcct gtcacacgag aattgtcatt ctgagcgcag cgaagaatct ccgtctcatt
     1861 tgggcaagcg acgagactct tcgctccgct cagagtgaca agcaaccggt aacagaccgc
     1921 tcaggccgcg gggaaggcct cccgcacggc cccggccagc ttccaggggt caaacgccac
     1981 caccggctgc gcgaacaact ccatggcccc gatgtcgctc gtggcgctgc cgggatcgcc
     2041 ccggtccacc atgcgcacca cgaccgggaa atccgtccag tcttcggcta ccggcgccag
     2101 cggcggcagg taacaggcca cgttgaaggc gcgcacgccc agcgtgtcga tcagcagccg
     2161 cagcagatcg gccaccgccg ccgccagggc gccgtcgaag gccggcgcca gcagcaccag
     2221 ttctttctcc ttgaccggcg tcaggtgggc catccagcga acgtcgccgt gccgcccggt
     2281 caggcccagc ccggcgtggg ccgcgtagaa gtccgcgaaa tagtcgcgcc cgtggcgggc
     2341 gcggtagccc tcggcggccc gccgccagag ttccacccgc ccgtaggcgc cgccttcgcc
     2401 cagcgtggcc tgcatatgcc cgtgcacctg cgaggcgccg ctgcgcgggc ggcagttcca
     2461 catcacgaag gggtataccg cctccgggcg ggctgccgct gcggcagcga tccagcgcgc
     2521 cgccgtatcg aaggcatcgg ccacctgcgc gccggaccaa gtcagcggat tgtgctcttt
     2581 gaagaggatc acggcgtgca gcgcgtcgta cttggcgacg ttgctggcgg taatggtgtg
     2641 cgtgccctca atgcgcccga aagtgtcggc gggcgtgccg gtcagcgggt ggcagaaggg
     2701 atcgtcgccg ccgcccgcca gttcggcatc ggcgttcgcg cccggcgcct gccgggccac
     2761 cgggcggcga gcacgcaggt cgttgaacag cgtgccctcc agcgtccagc ggttcagcgt
     2821 cttgatgatc gtctgggcct gcaccgcctc caccgagccg aaggaccgct cgatccaggc
     2881 gcgcatctgc ggcggcggct ggaggctccc gccgctgcgt gtcaccgcaa acaggtccgc
     2941 gaaccgggcg cgcccgtccg catcgagccc ggccacctgc tccggcaaat tggtgatcga
     3001 tcgcgaagcc tcgtcaatca tggcgcgcat agtaccatgt ttcgtgccac ggaacagcct
     3061 gccgcttgag cagtgccggc ttgccgccaa acccgtgtag ttgcacctcg cccccgcaag
     3121 tcatacgaaa atcatttcta ttaatccatt cttcgcgctc ttcgcgcctt tgcggtgttg
     3181 atcctttcca taaacaacct ctgcgtcctc tgcgcctcgg tggtgaggac gcgtggcagc
     3241 gcgagcaagc gaccttgaca accccagggg cctgcgctat cataagacta gtccaacacg
     3301 gccggtgcga gtcccatcga gacaacagcg acatacgcgg gatggggcca acccatcgcg
     3361 ccaggagaac accacatcga tgggcgagct gcgcagcgcc tcggtaacgt tggccgagat
     3421 catggcttac atcaaacaaa ctcgccagac cgggcggctg acagtgcgaa tccaggggcc
     3481 ggccggcacc gccgagggcc ccgccgcgct cattttcgag accggccacc tggtcgatgc
     3541 gcgcaatgcc actgagagcg gcgacgacct ggtctaccgc ctgctcggcc agcgcgaagc
     3601 cacctactcc tttgaccgtc tgtctgcgga ccagatcccg caggaacgca cgatcacccg
     3661 tgtgcaggaa ctgctgatgc tggccgccat cggcatcctg tccgaggaag atgccggccc
     3721 gtccgatagc gggcgcaccg aggcgatcga ggcggccacg cagcccagcc cgccgccgcc
     3781 gcccgcgccc gcgcctgtcg cgaaagaggc cccgctcccg ccgcctgccc ctaaaggggc
     3841 atctacagac gtaccgccgc gccgccgccc gcgctcgttc cggcgccgca acatgatccc
     3901 gctgccgccc ggccagccgg cctataccgg catggccccg ccgcgcgacc tgatgagcct
     3961 ggtcgatcgc ctggaacacg atctcttctc cggctacgtg acctggtttt cggaaggcgc
     4021 cgagggcttg ctcttgctct atcagggtca ggtgatcgac gccttctggg ccgaggtgca
     4081 gaccgccgct acctacgccg agcgcccggc catccgccag ttcgccgccg ccgtgcgcag
     4141 caacggccac aacccgatcg aggtctacga actggacccc gatttcgtct ggagttactc
     4201 ctcgctggcc tatggggcgg gcaaccccag ccggcagggc atggaccatg tgcagttgcc
     4261 ccaactgctg gtccatctgg cccacgtcga gcacaccggg tgcgtcaagg ttgtcaccgg
     4321 caaccaggcg gcctatgtct ttctctgcgg gggccggccc ctcggcgaat atcgcgccct
     4381 gccccactcg ctgcaaaccg cgccgggtcg cgccatgacc ctggccggta tgcccggctg
     4441 cctggtcgat gtctatacct cgcccacgcc cgccgagctc ctcgccctca acgcggccac
     4501 ctggccggtg gagcgcgtga tcgaggaatt gcgccgcacg gcccgcgatg tgctgggcaa
     4561 caaggccggg caggtgctga cgctgatcac gaacgcgggc agcgatccgg gggcactcca
     4621 cgcggcctgc gcgcgggcca agcaggtcac tcgcgtgttc atcgggcccg agaagcatgc
     4681 cgaactgagc cggcgtttgg atcgcctcct ggctcacttg cagtgagcca gaccagcagc
     4741 agtggtggaa tgggtactgt cgtcactccc gtaacgcaat ctcaggcggc gcccagcgac
     4801 ccgcacctga tggcctattt ccgggcctta cccgcctgcg agaccccgga cgcagcgctg
     4861 cgcctcacgc tggccggcgc gctggcgatg accggcgcgg ccagcggcgc ggccctggcc
     4921 gccgtcagcc cggccaagcc gctgaccgcc ggctcgcccg acggcgacct cgccgcgcag
     4981 atccaggccg cctgggccgc cgcggcgggc gctcccgcct tctttgcccc cgccgatccc
     5041 gcaccgccgg agagcgcggt ggacgccccg cgctacgtgc ccctggccgc gggcgggcgg
     5101 ctctacggcc tgctgggcct gcgcggcggg cccgcgctcg accccgccac cgccgatgcg
     5161 ctcgatgtca tggccgccgc taccgggcgc gccctgcaaa tgagcatcaa cctggacgaa
     5221 ctgcgcggac tgtacgaagt ggcgacggcc ttgaactcca cgctcgaact gccggtcctg
     5281 ctcaaggtga ttgtgaagcg caccaccgcc gccctgcgca gcgaagcctg cacgctgatg
     5341 ctgctcgacg agcgcaccca ggaactggtc ttcgagatcc ccgtgggccg ggccgaaaac
     5401 gccctgcgcc agttgcgtat gccgctgagc cagggcattt gcggctgggt ggcccgcaac
     5461 ggctgtcccg ccatcgtcaa cgacccgcgc gccgacgagc gcttccacac tagcgtcgat
     5521 cagagcagcg gcttcgtgac ccgcaacgcg ctggccgtcc cgctaatcct gcgggggcgg
     5581 gtcggcggcg tggtcgaggt gctcaacaag ctgggcaacg agccctacag cgcgcacgat
     5641 gtcgagttgc tgaccaccct ggccggccag gcggcggtgg cgctggataa cgcccagttg
     5701 tatacctccc tgcagatcga gcacgagcgc ctcctggccg ccgaggagca ggtgcgcaaa
     5761 gagttggccc gcgacctgca cgacggcccc gcccaacggc tggccgccat ctgcatggaa
     5821 gtcgagatcc tgcgcaagct gctggtgaag gatccctccc ggttgccgga ggagctggac
     5881 gcgctggaaa cgctggcccg caaggccaac cgcgaagtcc gcaccatgca gttccagttg
     5941 cgtccggtca tgctcgaaac ccgcggcctg cgcgccgctg tcgagtatta tgtcaaccaa
     6001 ttgcgcgaaa ccgagcgcgt cgccttcacc gtcgaagtcg cgggctgcga ggaccgccta
     6061 cccg
//
LOCUS       DASUYC010000676         9701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_552465,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000676 DASUYC010000000
VERSION     DASUYC010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 9701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9701
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552465"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..1082
                     /locus_tag="VF276_11045"
     CDS             174..1082
                     /locus_tag="VF276_11045"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEX6607439.1"
                     /translation="MTTTQTQVPMSRTRAWMLASRPATLPAAIVPVLVGAAIAVGQGQ
                     FRVGPFLGALVASLLIQIGTNFANDLFDFQKGADTHERLGPRRVTQSGLIPPDAVKRA
                     MILTFTAAAVIGLYLIAVGGLPILIIGVLSIISAVAYTGGPWPLGYHGLGEVFVFLFF
                     GLVAVTGTYYLQTGTVTPLALWASVPVGLLCTAILVVNNVRDIDTDRRAGKRTLAVRI
                     GRPATRALYTACLVVSYLITLGLWLLSPLTPLALLPWLTLPLAVPLIRTVWTQTAGPP
                     LNGVLKGTGRLHLLFGLLFAISLLWL"
     gene            1286..2887
                     /locus_tag="VF276_11050"
     CDS             1286..2887
                     /locus_tag="VF276_11050"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HEX6607440.1"
                     /translation="MDDFIVSSSEWVTMGRVASLFSIRPGAPARPQVMPDWLERQAAA
                     AGQAPAIIARGVRWSFADLDAVATRRAGQLCTLGVEPGDHIALLLGNSAEFAALVHAA
                     TKLDAVLVPLNIRLTPAELAWQLADSGARLLIHDGARTAAALAASADLPGLRCYGVGA
                     GLAKDTPALDAVSATWIDRHEGIDLDRVQSIIYTSGTTGRPKGAMLTYGNHWWSAIGS
                     ALNLGTHTGDRWLACLPLFHVGGQAILLRSAIYGVPAIIHEQFDPAAVNRAIDEDGVT
                     IISVVATMLARMLDERGDRPYPPTLRTVLLGGGPAPRPLLEACAARGVPVVQTYGLTE
                     AASQVATLAPGDALRKLGSAGKPLLPTQLRVVGPDGDRGPGEVGEIIVRGPTVTPGYL
                     GRPDATARALRGGWLYTGDLGYLDSEGYLYVVDRRDDLIISGGENIYPAEVEAVLLAH
                     PAVAEAGVIGVADARWGQVVCAFVRVRPAHAPAADELIAFCRERLARYKVPATIRFVE
                     TLPRNAAGKLLRRALRPDAAEEMQP"
     gene            2884..3927
                     /gene="aroF"
                     /locus_tag="VF276_11055"
     CDS             2884..3927
                     /gene="aroF"
                     /locus_tag="VF276_11055"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX6607441.1"
                     /translation="MIIVMKATAGARDVAAVAERMRALGFTPEVGEAAERVIVSVAAG
                     GGAAPEAELFSAMPGVDKIVALQPAYRLVSRAAHPHDTVIRAGDALIGGRNLAFMAGP
                     CSVENRAQTLEVAEYLAALGVKILRGGAFKPRTSPYSFQGLHETGLEILAEVRERTGM
                     AIITEVLSPQDVPLVAEHADILQIGTRNMQNFALLAAAGRQPRPVLLKRGMSATIEEW
                     LLAAEYIVSHGNSQVMLCERGIRSFDKSTRAVFDVGVIPLLKHLTHLPVIADPSHATG
                     RWDLVEPVAKAAVAAGADGLIVEVHAHPDQALSDGAQSLRPDRAADLLASLRRLTAAL
                     DRGLDLAPAALAA"
     gene            complement(4157..4609)
                     /locus_tag="VF276_11060"
     CDS             complement(4157..4609)
                     /locus_tag="VF276_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607442.1"
                     /translation="METPIGTWLSQLVLTHLDELAQEMATEARRTMPIYRAMDPEVVH
                     PLFVALYQVLAQSFAVGDTAPLRAYLERVTADRMRDGASADGFIKLAELSHQGLTRLI
                     RQELPQDPERAADAIRIAQNMNNNARLILSEINLRLINRRGGTRPLQS"
     gene            4812..5726
                     /locus_tag="VF276_11065"
     CDS             4812..5726
                     /locus_tag="VF276_11065"
                     /inference="COORDINATES: protein motif:HMM:NF018822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LppX_LprAFG lipoprotein"
                     /protein_id="HEX6607443.1"
                     /translation="MGKLKHVSLLVLLSMLLGLLAACGGETPTNTPVPPPPTNTPVPP
                     PPTNTTAPAAAATDTPAAGNSGTGSGTEATSAEMDLITQALTATQELKSYHYTLKASG
                     DIITETLDLQGDYVAPDKAYAKGTVGGETVEQLATGGKIYRKDAGGKWVETPESAAAG
                     AAANPADSVVQEANVLGSLSTFTSAGSNYRNVGTETLDGVSTTHFAGTIEASKMAGAE
                     GLGALGSIPSLGDINVWIDPQTKYIHKLDMNLDLSTFMGAIAGIAEALQGTPTPGGPT
                     ATPVPSLKFVVNLTVSKPNDPSISVPTP"
     gene            6174..8900
                     /locus_tag="VF276_11070"
     CDS             6174..8900
                     /locus_tag="VF276_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016876925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HEX6607444.1"
                     /translation="MDASTAESRRLEEHARRAADWRRWGPYLSERAWGTVREDYSADG
                     SAWTYFPHDHARSKAYRWGEDGLAGISDREGRLCFALALWNGRDPILKERLFGLTGPE
                     GNHGEDVKECYYYLDNTPTHSYMQTLYKYPQAAYPYAGLVAENGRRGRRAPEYELIDT
                     GVFAEHRYYDVFVEYAKVTPEDILIQIRVFNRGQDEETLHLLPTLWFRNTWAWGSDPA
                     RPALRRMGAQRGWQGIEAVHATLGTYWLYAESGATGPELLFTENETNMARLYNLPNAT
                     PFVKDSFHRYLIDGETGAVNAALTGTKAALHYQFTLAGGASAQIRLRLTNMGQDRPFG
                     RAFDAEFATRRAEADAFYAAIQPPSLPEDPRRVQRQALAGMLWGKQLYDYDVNRWMEG
                     DPAYPPPPAQREKGRNGDWCHLTAFDVISMPDKWEYPWFATWDLAFHTIPLALIDPDY
                     AKRQLLLMTREWYQHPNGALPAYEWAFSDVNPPVHAWAAWRIYQMEAAERGEGDRRFL
                     ESVFQKLLLNFTWWVNRKDVDGNNVFQGGFLGLDNIGVFDRSAPLPGGGHLEQADGTS
                     WMAAYCLNMLTIALELAGKDPVYQDIATKFFEHFLRIAAAMADVSEEGIALWDPQDGF
                     FYDALHMPNGSVQVLRVRSLVGLLPLFAVGLIEPELLERLPVFRRRMEWFLANRPFLS
                     GHIAEVGRPGVHKHRLFSILTPERLRLVLKRMLDENEFLSPYGIRSLSRYHLDHPFTL
                     TTECEEHTVSYQPAESQTGVFGGNSNWRGPIWFPVNYLIIESLLKFQHYYGDSFQVEC
                     PTGSGQMMTLAQVADHLAHRLIRLFLRDEQGRRPIYGDQALFQADPHWRDLILFHEYF
                     HGDNGYGLGASHQTGWTGLIAMLLQEHCETLHDLIDPYAEEAPLADAVSGAN"
     gene            8913..>9701
                     /locus_tag="VF276_11075"
     CDS             8913..>9701
                     /locus_tag="VF276_11075"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6607445.1"
                     /translation="MDEITIYGTTWCSDCKRTKKFLGEQRVAYSWVDVEESSDGLAFI
                     ESVQDGGHNVPTVKFGDGAVLVEPSNADLARKLGISTKAKRDFYDVVIIGGGPTGLMA
                     AIYLAREGVDCLIVERAGLGGQAGVTERLDNFPGWPDGIPGAELADRLAEQARRFGVE
                     ILAAQAVTSIETHDGYRDVHTAGGDEYGARAMLIASGSTYARLGVPGEEGFIGSGIHF
                     CATCDGPFYKGRPVMVIGGGNSAGEESIFLARFASAVTILVRGPK"
BASE COUNT         1591 a         3303 c         3303 g         1504 t
ORIGIN      
        1 cagccgtcag ctatcagggg acggcggaac gagatgtata ttggccgccg tcaggagatg
       61 ggagacgaat attgtcggct acaatcatcc tgatagctga tagctgatag ctgatagctg
      121 atagctgata gctgacggct gacggctgac ggctgatacc tcgaaggtac acaatgacga
      181 cgacacagac acaagttccg atgagccgca cgcgggcctg gatgctcgcg tcgcggcccg
      241 cgaccctgcc ggcggccatc gtgccggtgc tggtgggcgc ggctatcgcc gtgggccagg
      301 gccagttccg ggtggggccg ttcctggggg cgctggtggc ctccctgctg atccagatcg
      361 gcaccaactt cgccaacgac ctgttcgact tccagaaggg cgccgacacg catgagcgcc
      421 tggggccacg gcgggtcacg cagagcgggc tgatcccgcc cgacgccgtg aagcgggcga
      481 tgatcctcac cttcactgcc gccgccgtga tcggcctcta cctgatcgcg gtgggtgggc
      541 tgcccatcct gatcatcggc gtgctgtcga tcatcagcgc ggtagcctat accggcgggc
      601 cgtggccgct gggctatcat ggtctgggtg aggtgttcgt cttcctgttc ttcggcctag
      661 tcgccgtcac cggcacgtac tacttgcaga cgggcacggt cacgccgctg gcgctgtggg
      721 cgtcggtgcc ggtgggcctg ctctgcacgg ccatcctggt ggtcaacaac gtgcgcgaca
      781 tcgacaccga ccggcgcgcg ggcaagcgga cgctggcggt gcgcatcggg cgcccggcga
      841 cgcgagccct atacacggcc tgcctggtcg tgtcctacct gatcacgctg gggctctggc
      901 tgctcagccc gctgacgccg ctggcgctgc tgccctggct caccctgccg ctggccgtgc
      961 cgctgatccg caccgtctgg acgcagaccg ccgggccgcc gctgaacggc gtgcttaagg
     1021 gcaccgggcg gttgcatctg ttgtttgggt tattgtttgc gatcagtttg ctgtggttgt
     1081 gacgggggcg ggccggtttg aaaccggccc ctacaccgcg caatcagcag cgaggctggt
     1141 tctgagggac gccgggccgg tttgaaaccg gcccctacac cgcgtgagca gccggggcgg
     1201 ttccgggata cgccgggccg gtttcaaacc ggcccctaca ccgcgcaatg acctggccgg
     1261 cgcccagggc tgggaacgga taacgatgga cgattttatt gtatctagct cggaatgggt
     1321 gacgatgggg cgggtggcct cgctgttcag catccggccc ggcgcgcccg cgcgcccgca
     1381 ggtcatgccc gactggctgg agcggcaggc cgccgccgcc gggcaggccc cagcgatcat
     1441 cgcgcgcggc gtgcgctgga gcttcgccga tctagatgcc gtggcgaccc ggcgggcggg
     1501 gcaactctgc acgctgggcg tggagccggg cgaccacatc gccctgctgc tgggcaacag
     1561 cgccgagttt gccgcgctgg tccacgccgc caccaagctg gacgccgtgc tggtgccgct
     1621 gaacatccgg ctgacgcccg ccgaactggc ctggcaactg gccgactccg gcgcccggtt
     1681 gctgatccat gacggcgcac ggaccgcggc ggccctggct gcaagcgccg atttgccggg
     1741 cctgcgctgc tacggggtcg gggccggact ggcgaaagac accccggccc tcgacgccgt
     1801 gagcgcgacg tggatcgacc ggcacgaggg catcgacctg gaccgagtgc agagcatcat
     1861 ctacacatcg ggcacgaccg ggcggcccaa gggcgccatg ctgacctacg gcaaccactg
     1921 gtggagcgcc atcggctcgg cgctgaacct gggcacgcac accggcgacc gctggctggc
     1981 gtgcctgccc ctgttccatg tcggcggcca ggccatcctc ctgcgcagcg cgatctacgg
     2041 cgtcccagcc atcatccacg agcagttcga cccggcggcg gtaaaccggg ccatcgacga
     2101 ggacggggtg acgatcatct cggtggtggc caccatgctg gcgcgaatgc tcgacgagcg
     2161 gggcgaccgg ccctacccgc ccacgctgcg cacggtgctg ctgggcggcg gcccggcgcc
     2221 gcgcccgctg ctcgaagcct gcgccgcgcg cggcgtgccg gtggtgcaga cctacgggct
     2281 gaccgaggcc gcctcgcagg tggcgacgct ggcgcccggc gacgcgctgc gcaagctcgg
     2341 ctcggcgggc aagccgctgc tgccgacgca actgcgcgtg gtggggccgg acggcgaccg
     2401 ggggcccggc gaagtgggcg agatcatcgt gcgcgggccg acggtcacgc ccggctacct
     2461 gggccggccc gacgccacgg cgcgcgccct gcgtggcggc tggctctaca ccggcgacct
     2521 gggctacctg gacagcgagg gctacctcta cgtggtggac cgccgcgacg acctgatcat
     2581 ctcgggcggc gagaacatct atcccgccga ggtggaagcg gtgttgctgg cccaccccgc
     2641 cgtggccgag gcgggcgtca tcggcgtggc cgacgcgcgc tggggccagg tggtctgcgc
     2701 gttcgtgcgc gtgcgcccgg cccacgcgcc cgccgccgac gaattgatcg ctttttgccg
     2761 ggaacggctg gcgcgctaca aagtgcccgc cacgatccgg tttgtcgaga ccctaccgcg
     2821 gaacgccgcg ggcaaactgt tacgccgggc gctgcggccc gacgctgccg aggagatgca
     2881 gccatgatca ttgtgatgaa ggcgacggcc ggcgcccgcg atgtcgcggc tgtcgcggag
     2941 cgaatgcgcg cgctgggctt cacgcccgaa gtgggtgagg ccgccgagcg ggtaatcgtc
     3001 agcgtggccg cgggcggggg cgccgcgccg gaggccgaac tgttcagcgc catgcctggc
     3061 gtggacaaga tcgtggcgtt gcagccggcc tatcggctgg tgagccgggc ggcgcacccg
     3121 cacgacacgg tcatccgcgc gggcgatgcc ctgatcggcg ggcgcaacct ggccttcatg
     3181 gccgggccgt gctcggtcga gaaccgggcg cagacgctgg aggtggccga gtacctggcc
     3241 gcgctgggcg tcaagatcct gcgcggcggg gccttcaagc cgcgcacctc cccctacagc
     3301 ttccagggcc tgcacgagac cgggctggag atcctggccg aagtgcgcga gcggacgggc
     3361 atggcgatca tcaccgaggt gctgtcgccg caggatgtgc cgctggtggc cgagcatgcc
     3421 gacatcttgc agatcggcac gcgcaacatg caaaacttcg ccctgctggc agcggcgggc
     3481 cgccagccgc gcccggtgct gctcaagcgc ggcatgagcg cgaccatcga ggagtggctg
     3541 ctggccgccg aatacatcgt cagccacggc aacagccagg tgatgctctg cgaacggggc
     3601 atccgcagct tcgacaagag cacgcgcgcc gtgttcgacg tgggcgtgat cccgttgctc
     3661 aagcacctga cccacctgcc ggtgatcgcc gaccccagcc atgccaccgg gcgctgggac
     3721 ctggtcgagc cggtcgccaa ggccgccgta gctgcgggcg ccgacgggct gatcgtcgag
     3781 gtccacgccc accccgacca ggcgctgtcc gacggcgcgc aatcgctgcg cccggaccgg
     3841 gccgccgatc tgctggcgag cctgcgccgc ctcaccgccg ccctcgaccg cggcctcgac
     3901 ctcgcgcccg ccgcgctcgc cgcctgacga gtgctgagtg ctgagttgtg acgagtggtc
     3961 agtggtcagt ggtcagtggt cagtaatgtg gggaattgtc attctgagcg cagcgaagaa
     4021 tctccgtctg gtagcccaaa agacgagtcc cttcgctgca ctcagagtga caaagcgcgc
     4081 tgaatcgtca ttctgagcgc agcgaaggat ctccggcttg cttagtccgg gatgtagaca
     4141 gcgagaacag gtagggtcag gattgtagtg ggcgggtgcc gccgcggcgg ttgatcaggc
     4201 ggagattgat ttcgctgagg atcaggcggg cgttgttgtt catgttctgc gcgatgcgga
     4261 tggcgtcggc ggcgcgttcc ggatcctggg gcagttcctg gcggatcagg cgggtcagcc
     4321 cctggtgcga gagctcggcc agcttgatga agccgtcggc ggaggcgccg tcgcgcatgc
     4381 ggtcggcggt gacgcgttcc aggtaggccc ggagcggcgc cgtgtcgcct acggcgaagc
     4441 tctgcgccag cacctggtag agcgcgacga acaggggatg caccacctcc gggtccatgg
     4501 cccgatagat cggcatggtg cgccgggcct cggtcgccat ttcctgcgcc agctcatcca
     4561 ggtgggtcaa aaccagttgg gacaaccagg tgccgatggg tgtttccatg tgtctgcaac
     4621 tccaggctaa aacgaggatg gctggactca ttatggcata gcggggccga gtttgtcaat
     4681 ccgcccgtac cctccgcctt gacaaagcat tccataataa ggacaataag aggccggccc
     4741 gcgatgccgc tggtcctttg tccgggccgg cccccgatct gcgaatggac gttttaggag
     4801 ggagattctt catgggcaag ctgaaacacg tttcactgct ggtgctgctg agcatgctgt
     4861 tgggcctgct ggcggcctgt ggtggcgaga ccccgacgaa tacgccggtg cccccgccgc
     4921 cgactaacac cccggtaccg ccgccgccga ccaacaccac cgcgcccgcc gccgctgcga
     4981 cggacacccc cgccgccggc aatagcggca cgggcagcgg caccgaagcg accagcgccg
     5041 agatggacct gatcacccag gcgctgaccg cgacccagga gctaaagagc taccactaca
     5101 cgctcaaggc cagcggcgat atcatcaccg agacgctgga tctgcaaggc gactacgtgg
     5161 ccccggacaa ggcgtatgcc aagggcaccg ttggcggtga gaccgtcgag cagttggcaa
     5221 cgggcggcaa gatctaccgc aaggacgccg gcggcaagtg ggtcgagacg ccggaatcgg
     5281 ccgccgccgg cgccgccgcg aatcccgccg acagcgtggt gcaggaagcc aatgtgctgg
     5341 gcagcctgag caccttcacc agcgccgggt ccaactatcg caacgtgggt accgaaacgc
     5401 tcgacggcgt cagcacgacg cacttcgcgg gcaccatcga ggccagcaag atggcgggcg
     5461 ccgagggcct gggcgcgctc ggcagcattc cgtcgctcgg cgacatcaac gtctggatcg
     5521 atccccagac caagtatatc cataagctgg atatgaacct cgacctgagc acgttcatgg
     5581 gcgccattgc cggcatcgcg gaggccctgc aaggcactcc gacgcccggc ggcccgaccg
     5641 ccacgccggt gccgtcgctg aagttcgtcg tcaatctgac ggtgagcaag ccgaacgacc
     5701 cgtcgatcag cgtcccgacg ccgtaacagg cacgggcagg cacaccagcg ggggccggtc
     5761 ttgcgaccgg ccccttttcc atgcccgcgc ccggtcgccg cagatactca tcatcctctc
     5821 gtgcagcacc gcccgaagtt gcaatcagcc cgccgattga cagccgggct gttctggagt
     5881 acccttgcaa tagggcagcc ggtcatagca agcgcctggt cacgccaggg agcctgtcac
     5941 agggtatctg tcactctgag cggagcgaag agtctcgtcg ataacccgaa cgagacgaga
     6001 ttcttcgctg cgctcagaat gacaattctc cggggcgggc ctcgcgagcg cagcgacgag
     6061 tcgcgccgcg ccccggcccc accgattggc accctgggcc cggtatcgcg ttctcgctct
     6121 ggccgtgtgc tcaccaggca gccccggcca ggcggcgccg cgcaggagga catatggacg
     6181 caagcaccgc cgagtctcgc cggctggagg agcacgcccg ccgggcggcc gattggcgcc
     6241 gctggggacc ctacctcagc gaacgcgcct ggggcaccgt gcgcgaggac tacagcgccg
     6301 acgggtccgc ctggacctat ttcccccacg accatgcccg cagcaaggcc taccggtggg
     6361 gcgaggacgg cctggccggc atctccgacc gcgagggccg gctctgcttc gccctggccc
     6421 tctggaacgg ccgcgacccc atcctcaagg agcggctgtt cgggctgacc gggccggagg
     6481 gcaaccacgg cgaggacgtg aaagagtgct attactacct cgacaacacg cccacgcact
     6541 cctacatgca gacgctctac aagtatccgc aggcggccta cccctacgcc ggcctggtgg
     6601 ccgaaaacgg gcggcgcggg cgccgggcgc cggagtatga gctgatcgac acgggcgtct
     6661 tcgccgagca tcgctactat gatgtattcg tggagtacgc caaggtcacg ccggaagaca
     6721 tcctgatcca gatccgggtc ttcaaccgcg ggcaggacga ggagacactg cacctgctgc
     6781 ccacgctctg gttccgcaac acctgggcct ggggcagcga cccggcgcgg cccgccctgc
     6841 ggcgcatggg cgcgcagcgc ggctggcagg ggatcgaagc ggtccacgcc accctgggca
     6901 cctactggct ctatgccgag agcggagcga ccgggccgga actgctcttc accgagaacg
     6961 agaccaatat ggcgcggctg tacaacctgc ccaacgccac gcccttcgtc aaagacagct
     7021 tccaccgcta tctcatcgac ggcgagacgg gcgcggtgaa cgcagccctg accggcacca
     7081 aggccgcgct gcattaccag ttcaccctgg ccggcggcgc ctcggcgcag attcggctgc
     7141 ggctgaccaa tatgggtcag gatcgcccgt ttggccgggc gttcgacgcc gagttcgcca
     7201 cacggcgggc cgaagccgat gccttctacg ccgccatcca gccgcccagc ctgccggagg
     7261 acccgcgccg ggtgcagcgc caggcgctgg ccggcatgct gtggggcaag caactctacg
     7321 attatgatgt gaaccgctgg atggaaggcg acccggccta cccgccgcca cccgcgcagc
     7381 gcgagaaggg tcgcaacggc gactggtgcc acctcaccgc cttcgacgtg atctccatgc
     7441 ccgacaagtg ggagtacccc tggttcgcca cctgggatct ggccttccac accatcccgc
     7501 tggccctgat cgaccccgac tacgccaagc ggcaactgct gctgatgacc cgcgagtggt
     7561 accagcaccc caacggcgcg ctgcccgcct atgagtgggc cttcagcgac gtgaacccgc
     7621 cggtgcatgc ctgggccgcc tggcgcatct accagatgga agcggccgaa cgcggcgagg
     7681 gcgaccgccg cttcctggag agcgtgttcc aaaagctgct gctcaacttc acctggtggg
     7741 tcaaccgcaa ggacgtggac ggcaacaacg tgttccaggg cgggttcctg ggcctggaca
     7801 acatcggcgt gttcgaccgc agcgcgccgc tgcccggcgg cggccacctg gaacaggccg
     7861 acggcacgag ctggatggcc gcctactgcc tgaacatgct gaccatcgcc ctggaactgg
     7921 ccgggaaaga cccggtctac caggatatcg ccaccaagtt cttcgagcac ttcttacgca
     7981 tcgccgcggc catggccgac gtgtccgagg aagggatcgc gctctgggac ccgcaggacg
     8041 gcttcttcta cgatgccctg catatgccca acggatcggt ccaggtgctg cgcgtacgtt
     8101 ccctggtagg gctactgccg ttattcgccg tgggcctcat cgagccggag ttgctggagc
     8161 ggctgccggt cttccggcgg cgcatggagt ggttcctcgc caaccggccc ttcctcagcg
     8221 gccatatcgc cgaggtgggc cggcccggcg tccataagca ccgcctcttc tcgatcctga
     8281 cgcccgaacg gctccgattg gtgctgaagc gcatgctcga cgagaacgag ttcctgtcgc
     8341 cctacggcat ccgctcgctc tccagatacc acctggacca tcccttcacg ctgaccaccg
     8401 agtgcgaaga gcatacagtc agctaccagc ccgccgagtc gcagacgggc gtcttcggcg
     8461 gcaactccaa ctggcgcggg ccgatctggt tcccggtgaa ttacctgatc atcgagtcgc
     8521 tgctcaagtt ccagcattac tacggcgaca gcttccaggt cgagtgccct accggctccg
     8581 gccagatgat gacgctggcc caggtggccg accacctcgc ccaccgcctg atccgcctct
     8641 tcctgcgcga tgagcagggt cgccgcccga tctacggaga ccaggcactg ttccaggccg
     8701 acccgcactg gcgcgacctg atcctgttcc acgaatactt ccacggtgac aacggctatg
     8761 gcctcggcgc cagccaccag accggctgga ccggcctgat cgccatgctg ctgcaagaac
     8821 actgcgagac gttgcacgac ctgatcgatc cctacgctga agaagcgccg ttagcggacg
     8881 cggtcagcgg cgcgaactga aaggaagcgg gtttggacga aatcaccatt tatggcacca
     8941 cgtggtgctc ggactgcaag cggaccaaga agttcctggg cgagcagcgc gtggcctata
     9001 gctgggtgga cgtggaggaa agtagcgacg gcctggcctt catcgaatcg gtgcaagacg
     9061 gcgggcacaa cgtgccgacc gtcaagttcg gggacggcgc ggtgctggtc gagccgtcga
     9121 acgccgatct ggcgcgcaag ctgggcatct ccaccaaggc caagcgcgat ttctacgatg
     9181 tggtcattat cggcggcggg cccaccgggc tgatggccgc gatctacctg gcccgcgagg
     9241 gcgtggactg cctgatcgtg gagcgggccg ggctgggcgg gcaggccggc gtgactgagc
     9301 ggctggataa cttccccggc tggccggatg gcattccggg cgctgaactg gccgaccggc
     9361 tggccgagca ggcgcgccgc tttggcgtcg agatcctggc cgcccaggcg gtgaccagca
     9421 tcgagaccca cgacggctac cgcgatgtcc ataccgccgg cggcgacgaa tacggcgcgc
     9481 gggccatgct gattgcttcc ggctcgacct acgcccggct gggtgtgccc ggcgaggagg
     9541 gcttcatcgg cagcggcatc cacttctgcg ccacctgcga cggccccttc tacaagggcc
     9601 gcccggtgat ggtcatcggc ggcggcaaca gcgcgggcga ggagagcatc ttcctggccc
     9661 gcttcgccag cgccgtcacg atcctggtgc gcggcccgaa g
//
LOCUS       DASUYC010000677         2909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1555446, whole genome shotgun sequence.
ACCESSION   DASUYC010000677 DASUYC010000000
VERSION     DASUYC010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1555446"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="VF276_11080"
     CDS             complement(<1..942)
                     /locus_tag="VF276_11080"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6607446.1"
                     /translation="MISPQLRALARCPTCTAEMGGHAPPLADAGAGLLFCPRCDRRYP
                     LAPGGVYVDLMPRAAGDLGHTSRYVADLAEFAERLDYREMAPPLLGAAVRNRLLRQML
                     ALNPRDRVLELGCGNGKFLLWNRDLVRESVGIDPAPLFADEALVTLDLVQGDARLLPF
                     PNNSFSKVWSIDVMEHLDHADIVRYLAEAYRVLAPGGRMFIFSNTRERGPFDFLVSGS
                     RRLADWLAQRGLADPRRDLLRKSDHLKVLEVWEDVEDAVTAAGFEIRRVVYWNGVFQS
                     FLDNVVLRLAERLLVGLRGRKAAATPAPETAPPAFQDA"
     gene            complement(942..2363)
                     /locus_tag="VF276_11085"
     CDS             complement(942..2363)
                     /locus_tag="VF276_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607447.1"
                     /translation="MTAALAVLRRDRGLLALLVVMLLGIPFVTARIYASDEVKYFAYL
                     HSLSFDHDLDFTNDYHYWLDVKKYADIASLLKPNPTTGLPINEAPIGTALLWAPAFAL
                     AHVGVLAARALGARVAADGYSQPYIAAVCYASYFYGWAGLLLAYGLLRRFCDQFLAAL
                     ATLTIWLGTSAFFYMIIAPPWSHAASLFTVALFTTVWFRTRASEGRTWRQWALLGFCA
                     GLMMMVREQDALFLLLPAVETLFRLARLWRARAEATFGALWPALRRLAGGWALMLGVA
                     LVTFTPQLIAYWVLGGRLGPSSTVGEKLNWLSPHALDVLLSPQYGIIPWAPVVVPALI
                     GLALLARRDRELALALLVAFAAQLFAAGAYSTWQGKSSFGQRRFVNCTLLFIVGLGAL
                     LQWARTRGTRPALLAAMAAVFVAWEGGLLLQYAQLWDSARKQAGLQWPGVLADQARAY
                     GRLPELAWRYVFDRKSLVNRGLQ"
     gene            complement(2360..2707)
                     /locus_tag="VF276_11090"
     CDS             complement(2360..2707)
                     /locus_tag="VF276_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607448.1"
                     /translation="MPRIGVIVSYKHRPGMLKELQEWLNGQGAAMLSAACGANERYIG
                     VYMIEGDPTYSIQVHTETTDPSVLEDLELLNSPKFTRGQTAMKLMWRFLDQSVPPRVH
                     FIRPLHEAPPPRT"
BASE COUNT          497 a         1019 c         1010 g          383 t
ORIGIN      
        1 ggcgtcctga aaggcgggtg gcgcggtctc cggggccggg gtggcggcgg ctttgcgccc
       61 gcgcaggccc accagcaggc gctcggccag gcgcagcacc acgttgtcga ggaagctctg
      121 gaacacgccg ttccagtaga ccacgcgccg gatctcgaac cccgccgccg tcaccgcgtc
      181 ctcgacatcc tcccagactt ccagcacctt caggtggtcc gacttgcgca gcaggtcgcg
      241 gcgcgggtcg gccaggccgc gctgggccag ccagtcggcc agccgccgcg aaccggagac
      301 caggaagtcg aacggcccgc gctcgcgggt gttggagaag atgaacatgc gcccgcccgg
      361 cgccagcacg cgatacgcct cggccaggta gcggacgatg tcggcgtggt ccaggtgctc
      421 catcacgtcg attgaccaga ccttgctgaa gctgttattc gggaacggca gcagccgcgc
      481 gtcgccctgc accaggtcga gcgtgaccag cgcctcgtcg gcgaacagcg gcgccggatc
      541 gatgcccacg ctctcgcgca ccaggtcgcg gttccagagc aggaacttgc cgttgccgca
      601 gcccagctcc agcacgcggt cgcgcgggtt gagcgccagc atctgccgca agaggcggtt
      661 gcgcacggcg gcgcccagca gcggcggggc catctcgcgg tagtccagcc gctcggcgaa
      721 ctcggccagg tcggccacgt agcgcgaggt gtggcccagg tctccggcgg cccgcggcat
      781 caggtccacg tacaccccgc cgggcgccag gggatagcgc cggtcgcagc gcgggcagaa
      841 gagcagcccc gccccggcat cggccagcgg cggggcgtgc ccgcccatct cggccgtgca
      901 ggtgggacag cgcgccagcg cgcgcagttg cggtgaaatc attattggag acctcgattc
      961 accagggact tgcggtcgaa cacatagcgc caggcgagtt ccggcaggcg gccataggcc
     1021 cgcgcctggt cggccagcac gcccggccat tgcagcccgg cttgcttgcg cgccgagtcc
     1081 cataactggg catattgcag cagcagcccg ccctcccagg ccacgaacac cgccgccatg
     1141 gccgccagca gcgcgggacg ggtgccgcgc gtgcgcgccc actgcaacaa ggcgcccaga
     1201 cccacgatga acagcagcgt gcagttcacg aagcggcgct gcccgaacga gcttttgccc
     1261 tgccaggtgg aataggcccc ggcggcgaaa agctgcgcgg caaaggcgac cagcagagcc
     1321 agcgccaatt cgcggtcgcg ccgggccagc agcgccaacc cgatcagcgc gggtaccacc
     1381 accggcgccc aggggatgat gccgtattgc ggcgacagca gcacgtccag cgcgtggggc
     1441 gacagccagt tcagcttctc gccgaccgtg ctcgacggcc ccagccgccc gcccagcacc
     1501 cagtaggcga tcaactgcgg cgtgaacgtc accagcgcca cgccgagcat gagcgcccac
     1561 cccccggcca gccgccgcag cgcgggccag agcgcgccga aggttgcctc ggcccgcgca
     1621 cgccacaggc gcgccagccg gaacagcgtc tcgaccgcgg gcagcagcag gaacagcgcg
     1681 tcctgctcgc gcaccatcat catcagcccg gcgcagaagc cgagcagcgc ccactgccgc
     1741 caggtgcggc cttcggacgc gcgcgtgcgg aaccagaccg tcgtgaacag ggccaccgtg
     1801 aacagcgagg cggcgtgcga ccagggcggg gcgatgatca tatagaagaa ggccgaagtg
     1861 cctagccaga tggtcagcgt cgccagcgcg gccaggaact ggtcgcagaa gcggcgcagc
     1921 aagccgtagg ccagcagcaa cccggcccag ccgtagaagt aggatgcgta gcacaccgcg
     1981 gcgatatagg gctggctgta gccgtcggcg gccacccgcg ccccgagcgc ccgcgccgcc
     2041 agcacgccca cgtgggccag cgcgaaagcc ggcgcccaga gcagcgccgt gccgatcggc
     2101 gcctcgttga tcggcagccc cgtggtcggg ttcggcttga gcaggctggc gatgtcggcg
     2161 tacttcttca cgtcgagcca gtagtggtag tcgttggtga aatcgaggtc gtggtcgaag
     2221 ctcagcgaat ggaggtacgc gaagtacttg acctcgtcgg aggcgtagat gcgcgcggtg
     2281 acgaacggga tgccgagcag catgacgacg agcagcgcca gcaagccccg gtcgcgccgc
     2341 aaaacagcga gggcagcggt catgtgcgcg ggggcggcgc ctcgtgcagc gggcggatga
     2401 agtggacccg cggcggcacc gattggtcca ggaaccgcca catgagtttc atggccgttt
     2461 gcccgcgcgt gaacttgggg ctgttgagca gttcgaggtc ttcgagcacg gaggggtcgg
     2521 ttgtctcggt atgcacctgg atgctgtagg tcgggtcgcc ttcgatcata tagacgccga
     2581 tgtagcgctc gttcgcgccg caggcggcgc tcagcatcgc cgcgccctgc ccattgagcc
     2641 attcctgtag ctctttgagc atgccgggcc ggtgcttgta gctgacgatg accccgatcc
     2701 tgggcatggc gaccgcgtcc tctctctcgt cggggcccgc gcaacgcggc tcccggcatc
     2761 ataccacgcg caaacctcgc gcacggcgta tcgattatac ggcccgcgcc gcctcgctgt
     2821 caaccgcacc cgcgccggca ggactcgtgg gcggcgcgat cttgcaagga atccgggttc
     2881 ttgatacact ggggcggaag catccaacg
//
LOCUS       DASUYC010000678         4953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_872521,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000678 DASUYC010000000
VERSION     DASUYC010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872521"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VF276_11095"
     CDS             complement(<1..323)
                     /locus_tag="VF276_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607449.1"
                     /translation="MAQSVAPGATNHPLPPPDGTPAVARAAGILTDTAHRPGAASRSR
                     MAAAMQGAVGNARLNRMLGVPPPAGRSGDLVNRAAAAPARPESPAGDLAVSHPDSPAE
                     REAEAV"
     gene            complement(558..4082)
                     /locus_tag="VF276_11100"
     CDS             complement(558..4082)
                     /locus_tag="VF276_11100"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6607450.1"
                     /translation="METRTGNMHDFPRVAEAAGDEQGLLLVAEAADNAESLLLVEDRT
                     NNEQRLLLVEEVPATEQRLLLVEEVPDTEAGGLLVAEGTGGREQLLLVAEAADHTADK
                     QLMAHDSGTEQLLFIEEVTDQQEQLPFIEEVAGTREQLLFIEEVANQREQPARDPDAG
                     AGADSAGGGEQVRDRTEHLLLVEEAGAAGDPAPRVEEATNGEAHLRSVEETATGTDPE
                     LLVEEDINDVEVLLPAEEVKSDATPVPRAEEAPDHADNPPLVTEALDSTDSEVVIVEV
                     ETGAFRAADAQEPAGMYDRLLSPVIVLSSAAYETSMEHLWDELQRVDLLVRAQTVLWL
                     QTIGDYKPENLWGMVHVTDAEVAAYLQAPFARPAATRAAYPGAVGDYEAAAANMAAWI
                     AARHACGDPPEPLRLAQLCRLFHLSPVERDVLLICLLPEIDGRYRRLFGYLQDDASRT
                     RPTIELALQILVPHGLDAESGRKLFAAGGSLTRCGLLVAAGDTPGDEPLPLRSIRIDD
                     RIAGFLLGNDEPDARLVNILAEYPCTRPANWDELIIDQKRKAALQDFARWCQNQRVHA
                     AGGATLLLHGPYGSGRRTAACAICADSGTDLLVADVGAALRSPVLFEQIVDLAYREAR
                     LRGAAIYWAGCEQLLEPDQPAARWQHLVAAAEGFAGLTFLASSTAWDPADRFHDQVFL
                     RLEFPIPTYDLRVTLWRRYLRAVRFADPQIDREALAELLANSFQLTAGQMRDALATAR
                     ELAAVRMLGDHGDPAQPALRVDDLYDGCRRQSGQRLIAFARRVEPSTQLTFADLILPD
                     TNKQQLEELRRRIRYRSQVFSGLGFERRLTLGNGLTALFTGSSGTGKTMAAGLLAREQ
                     RVDLYKIDLSSVVSKYVGETEKNLSRIFAEAENSNAIIFFDEADALFGKRGEVKEAQD
                     RWANIEVNFLLQRIEEYAGVVILATNLRQNIDEAFLRRIHRIVEFPFPDEHARKSIWI
                     GMFPAGLGRPSDDDLGDLAKRFALPGGSIRNIVLDATFLALTANSTNAGGATQVRVEH
                     LVRATAREYQKLGKPLNKGEFGEEYYEWIEDKILLLPPPDRDTRFRIWLEAFPPEVVM
                     PSSRNLRDLADRFKLRQYSIQSAVRAAIAHARRAGSVGADGRLIVTLAHLALGLAETQ
                     RDQGLPVTCDLFGDELYELAVGSADFAV"
     gene            complement(4232..>4953)
                     /locus_tag="VF276_11105"
     CDS             complement(4232..>4953)
                     /locus_tag="VF276_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607451.1"
                     /translation="TAAWPAGATTMRAAQAGFQAACLRALVGQRLVQTPAIHLHQMRL
                     PTGDLALILSDDSATLWPLGRIAAAEEVQDVVAEWLALWVDIHGRPPAWVVADAPLAG
                     RLPDVAPIMAVGVDSGGELEEAHRAGQERLAAALAAVHYGRLGLPEVDLTLDLLAIAL
                     LRLWARWLRQFGGSSVPYLLANFVRRPGRIYSAPGQLTLELPALPLDIVLEMASYTAT
                     LARVPWLGGRHLHFTMGAPER"
BASE COUNT          782 a         1680 c         1623 g          868 t
ORIGIN      
        1 acggcttcgg cttcgcgttc cgccgggctg tcggggtggc tgacggccag atcgcccgcc
       61 ggagattcgg ggcgcgcggg cgcggcggcg gcgcggttca cgagatcgcc ggaccggccc
      121 gcgggcggcg gtacgcccaa catacggttc aggcgcgcgt tgccgacggc gccttgcatg
      181 gccgccgcca tgcgcgaacg gctggccgcg cccggccggt gggcggtatc ggttaagata
      241 ccggccgccc gcgctaccgc gggcgtgccg tcgggtggcg gcaagggatg gttcgtcgcg
      301 ccgggcgcga cggattgtgc caaccggctc accggtccct tgcggacgtc cggttgcttg
      361 gccgccacgg ggagcgcctg cgcgaggctg tgcgcctcga tttgtgtttt gcccgctacg
      421 gacatattac ctcaactacc cagttggcgg cgcaagccgg cttgcgccgg tattccacca
      481 cgccgatgct gctgggctgc agcccggttg caaaccggcc cctacatctg gcgtccaggc
      541 cagttggaaa ccggctccta caccgcgaaa tcagcggagc cgactgctaa ttcgtaaagc
      601 tcatcgccga acagatcgca tgtgaccggc agaccctggt cgcgttgcgt ttcggcaagg
      661 ccgagcgcca ggtgcgccag ggtcacgatc agccggccgt cggcccccac gctgcctgcc
      721 cgccgggcat gggcaatggc cgcccgcacg gcagattgaa tgctatactg ccgcagcttg
      781 aagcgatccg ccaggtcgcg gaggttccgg ctacttggca tcaccacttc gggcgggaac
      841 gcctccagcc agatgcggaa tcgggtatcg cgatctggcg gcggcagcag gaggatctta
      901 tcctcgatcc actcgtaata ctcctcgcca aactccccct tgttgagcgg cttgcccagt
      961 ttttggtatt cgcgcgccgt tgcccgcacc agatgctcga ctcgcacctg ggtggcgccg
     1021 ccggcgttcg tgctgttggc tgtcagagcc aggaaggtcg cgtccagcac gatgttgcgg
     1081 atgctgccgc ccggcaaggc gaagcgtttg gcgagatcgc cgagatcatc gtcgcttggc
     1141 cggcccagcc cggcggggaa catcccgatc cagatagatt tacgggcgtg ctcatcgggg
     1201 aacgggaatt cgacaatgcg gtggatgcgg cgcaagaacg cctcgtcgat gttctggcgc
     1261 aagttggttg ccaggatgac gaccccggca tattcctcga ttcgttgcaa caggaagttg
     1321 acctcgatat tggcccagcg atcctgcgct tccttgacct cgccgcgctt gccgaagagg
     1381 gcatcggctt cgtcaaagaa gataatggcg ttggaattct cggcttcggc gaagatgcgg
     1441 ctcaggttct tctcggtttc cccgacatac ttgcttacca cgctcgacag gtcgatttta
     1501 taaagatcca cccgctgctc gcgggccagt agaccggccg ccatcgtctt gcctgtgccg
     1561 gacgacccgg tgaagagggc ggttagcccg ttgcccagcg tcaggcgccg ctcaaagccg
     1621 aggccgctga atacctggct gcggtagcgg atgcgccggc gtaattcctc tagttgctgt
     1681 ttattggtgt cgggcaggat cagatcggca aaggtgagtt gcgtgcttgg ctccacccgg
     1741 cgcgcgaagg cgatcagccg ctggcccgat tggcggcggc agccatcgta caggtcgtcc
     1801 acccgcaggg ccggctgggc cggatcgcca tggtcgccca gcatacgtac agcggctaac
     1861 tcgcgggcgg tcgccagcgc gtcgcgcatc tggcccgccg taagctggaa gctgttcgcc
     1921 agcagttcgg ccagggcctc ccggtcgatc tgcgggtcgg cgaagcgcac cgctcgcagg
     1981 tagcgccgcc agagtgtcac gcggagatcg taggttggga tcgggaactc cagccgcaag
     2041 aacacctgat catggaaccg atccgccgga tcccaggcgg tgctgcttgc caggaacgtc
     2101 agtccagcga atccctcggc tgctgccacc agatgttgcc agcgggccgc gggctggtca
     2161 ggttccagca attgctcaca gccggcccaa taaatcgccg cgccgcgcag gcgcgcttcg
     2221 cgataggcga gatccacgat ttgctcgaag aggaccgggg accgcaaggc cgcacccacg
     2281 tcggctacga ggaggtcggt tccactgtcc gcacaaatcg cgcaagcggc tgtccggcgc
     2341 ccgctgccat aaggcccatg tagcaacagc gtggccccgc cggccgcatg cacgcgctga
     2401 ttttggcacc agcgggcgaa gtcttgcagc gcggctttgc gcttttgatc aataatcagc
     2461 tcatcccaat tagcaggtct cgtacacgga tactccgcca ggatgttcac gagccgggcg
     2521 tccggttcat catttccgag cagaaagccg gcgatgcggt cgtcgatgcg gattgaacgg
     2581 agcgggagcg gctcatcgcc gggtgtatcg ccggccgcga ccagcagacc gcagcgggta
     2641 agagacccgc ccgccgcaaa cagtttccgc ccgctttcgg catccaggcc atgggggacg
     2701 agaatttgta gcgccaactc gattgtgggt cgggtgcgcg aggcatcgtc ctggagataa
     2761 ccgaatagac gacggtagcg gccgtcgatc tccggtagga ggcagatgag cagcacatcc
     2821 cgttcgactg gggaaaggtg gaagagccgg catagctgcg ccagccgtag cggttccggc
     2881 ggatcgccgc aggcgtgcct ggcggcaatc cacgcggcca tgttcgccgc cgccgcctcg
     2941 tagtcgccca cggcaccggg gtatgccgct cgtgtagcag cgggccgcgc gaagggcgct
     3001 tgcaggtacg cggccacctc ggcgtcggtg acatggacca tgccccacag gttctccggt
     3061 ttgtagtcgc cgatggtctg caaccagagc accgtctgcg cccgcaccag gaggtcgacg
     3121 cgctgcaact cgtcccagag atgctccata ctcgtctcat atgccgccga agacagtacg
     3181 atcaccgggg acaacaggcg atcatacatt ccggccggct cctgcgcatc agcggcgcgg
     3241 aacgcgcctg tctcaacttc cacaatgacc acctcgctgt ctgtgctgtc cagggcttcc
     3301 gtgaccagcg gcgggttatc tgcgtgatcc ggggcttcct cggcccgcgg cacgggcgtg
     3361 gcatcgctct taacttcctc cgccggcagc agaacctcca cgtcgtttat atcttcttcc
     3421 acaagtagct cagggtctgt gccggtggcg gtttcctcga cggaccgcag gtgggcctcg
     3481 ccgttcgtag cttcctccac gcgcggggcg gggtccccgg cggcgccggc ttcttccacc
     3541 agcagcagat gctccgtgcg gtccctgact tgctccccgc ccccggcgct atccgcgccg
     3601 gcgccggcat ccgggtcgcg cgctggttgc tctcgctggt tggcgacttc ctcgataaac
     3661 aggagttgct ctcgtgtgcc ggcgacttcc tcgatgaatg ggagttgctc ctgctggtca
     3721 gtgacttcct cgatgaacag aagttgctcc gtgccgctgt catgcgccat gagttgttta
     3781 tctgccgtgt ggtccgcggc ttccgctacg agcaacagtt gctccctgcc cccggtgcct
     3841 tccgcgacca gcagcccgcc ggcctcggtg tccggcactt cctcgaccag cagcaggcgc
     3901 tgctcggtgg ccggcacttc ctcgaccagc agcaggcgct gctcgttgtt cgtgcggtcc
     3961 tccacgagca gcaggctctc cgcattgtct gccgcttccg cgacgagcag caggccctgc
     4021 tcgtcgcctg cggcttccgc cacccgtgga aaatcgtgca tgttgccggt tctggtctcc
     4081 attgcatcac catccctatc gccgtccggc gcgaccgggc ctactgcacc cggctccaag
     4141 actccggtgg gcggcagcgt ggccgtcaga gccggcgggc ggcggcgcca tccgcgggtc
     4201 agccaggtcc acagcgcggt tagccatcgt atcatcgctc cggcgctccc atcgtgaaat
     4261 gcaggtgccg cccgccgagc cacggcacgc gggccagggt tgcggtgtag ctggccatct
     4321 ccagcacgat atccagcggc aaggccggta gttccagggt cagttggccg ggcgcgctgt
     4381 agatgcggcc ggggcggcgc acgaagttcg cgagcaggta gggcacgctg gaaccaccga
     4441 actggcgcaa ccagcgcgcc cacaggcgca acagggcaat cgccagcaga tccagtgtga
     4501 gatcgacttc gggcaacccc aggcggccat agtggacggc ggccagcgcc gccgccagcc
     4561 gttcctggcc cgcgcgatgc gcctcctcca actccccgcc gctatcgacc cccaccgcca
     4621 tgatcggggc cacgtccggc aggcgaccgg ccagcggtgc gtcggcgact acccaggcgg
     4681 gcggccggcc gtggatatcc acccagagcg ccagccattc ggccaccacg tcctgcacct
     4741 cttccgccgc cgcgatgcgc cccagcggcc acagcgtcgc gctgtcgtcg ctcagtatca
     4801 gggccaggtc accggtgggt aggcgcatct ggtgcagatg gatcgccgga gtctggacca
     4861 ggcgctgccc gaccagcgcg cgcaggcagg ccgcctggaa gccggcctgg gcggcacgca
     4921 ttgtcgtcgc gccagcgggc catgccgccg tcc
//
LOCUS       DASUYC010000679         4635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1579899, whole genome shotgun sequence.
ACCESSION   DASUYC010000679 DASUYC010000000
VERSION     DASUYC010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1579899"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="VF276_11110"
     CDS             complement(<1..1163)
                     /locus_tag="VF276_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607452.1"
                     /translation="MRNRTAGRTATTAEMGGPAPETATGTATHAAAPPRPLGRRLLAA
                     LWPVAVFTVLTVIMTWPLVTDLQHGIIYGITADADVSRWDLWWFKTALLERHSNPFYT
                     DMLYYPYRLGANALPLYFHTLQPLNGLLALPVLLLTDDVVGPTFAYNLLLLGHMILTG
                     CAMFWLVRYLTGSAAGALVAGIVFTFGAFHQYHIHEAQLELIPLEWLPLFVLFLHRLL
                     YAAPDRRRRMLNGVVAVAVLVATALTSWYLTLYLLMIGGLLALVRLVERPRDWRATLG
                     ATAAVMLAWGVLVGPFLYATIRSSADPNFQLVSGLDYEVRFSLSPLDLFTVSKDVRMD
                     PAVWFLGPLGWSALVLGAVGAWRLRRRAIFWGILIGVGAILALGPYLQWDAAI"
     gene            1431..>4635
                     /locus_tag="VF276_11115"
     CDS             1431..>4635
                     /locus_tag="VF276_11115"
                     /inference="COORDINATES: protein motif:HMM:NF042648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="HEX6607453.1"
                     /translation="MFQHLHKPAPIRLIGMLTLVCAFVLLGAGLWGALNPATRTQAAA
                     PHSAARTANNRIVTENALPGTDEWADIGWNDPPKLNAYAGATSVNAGNPISIYVNNAG
                     SSITWRLYRLGYYQNHGARLITTGGPVATSPQPACTRTSSTGLVRCPWAAAFTLPTNA
                     AWVSGIYLLRMDSDQGRRIYVYFTVRNDTYNADFVFQEASMTNQAYNDYGGESLYLSR
                     NNEGRPRGYQVSFDRPYADGGGTGSFFTHDVDMLRWVEASGYDVTYISDVDTATNPNI
                     LLNHKVVLEVGHPEYWTWGERDNVENALAAGVNMVFASANETYWSVRLESSPVGPNRI
                     ITCYKDPALDPTHTAPYTTVEFDGPILQRPDNILVGVGYQSYYDDARYNYPWVISAPA
                     TRWYFDCTGLQPGDQVNNIIGEEWAAVHDNGHTPPGLDVISSGTVINAHGVGLAQNSA
                     IYTAASGAKVFAAGSIHFAWGLIDHSYPNQVFQSYALSNDADHRVEQFMANILDNFAG
                     YWDGSPRTCGGQGYYDVGTRATRTPMPQVPTATGTPPTATRTPTRTPTPVSGTATNTP
                     VGTPTNTPTATPTSPPVQGAITTSDPTQAGRFVRDWTVASCGTVKTAPQLADSTARHY
                     DAYQYVNNTGSSQCVTVRLTTACTGPNDIFSAAYLGSFTPANIVTNYRSDIGASPGQL
                     GGSSSYGFDVPAGQTFVVTVYEVTANAGCGSYTLAVTGGSPVGGATSTPTATVAATHT
                     PTATRTATASATPARTATATSTATATSTSLPPSATATAIPSATSTAIPTGTATDTPLP
                     SATATRTPSHTATVPPTATDTPAVSPSSTSTATAIVSPTLTATAVPPTRTATATVTAT
                     APPNSTATRTATSTRTATRTPTSTRTTTPTRTATRTPISTATRTPTGIATSTATRTPI
                     NTGTATPTVTATPGGGPQPIQGAITSGDPTQAGRIVRDWVPSTCSTVKTVPGNGDMLA
                     HHYDAYTFANNTAGTACVTVQLTSPCTAGNDIFSIAYLDSFDPANITANYRGDIGWSP
                     GQDTGSNSYSFTVAAGRHFVVTVYEVTANGGCGSYTLN"
BASE COUNT          880 a         1707 c         1422 g          626 t
ORIGIN      
        1 tcgatggcgg cgtcccattg cagatacggg ccgagggcca ggatggcgcc cacgccgatc
       61 aggatgcccc agaagatcgc ccggcgccgc agccgccacg cgcccacggc gcccagcacc
      121 agcgcgctcc agcccagcgg ccccaggaac cagaccgccg ggtccatgcg tacgtcttta
      181 gacaccgtga agaggtcgag cgggctgagc gagaagcgca cctcgtagtc gagcccgctg
      241 accaactgga agttggggtc ggcgctggaa cggatcgtcg cgtacaggaa cggcccgaca
      301 agcacccccc aggccagcat caccgccgcc gtggcgccga gcgtcgcgcg ccaatcgcgc
      361 gggcgctcca ccaggcgcac cagggccagc agcccgccga tcatcagcag gtagagcgtc
      421 aggtaccaac tggtcagcgc cgtggcgacc agcacggcca cggccacgac gccgttgagc
      481 atccgccgcc ggcgatccgg cgcagcatag agcaggcggt gcaggaacag cacaaagagc
      541 ggcagccact ccagcgggat cagctccaac tgggcctcgt ggatgtggta ctggtggaac
      601 gcgccgaagg tgaagacgat cccggcaacc agcgcgcccg ccgcgctacc ggtcaggtag
      661 cgtaccagcc agaacatggc gcagccggtc aggatcatgt ggcccagcag cagcaggttg
      721 taggcgaagg tcggcccgac cacgtcgtcc gtcagcagca ggacgggcag ggccagcagg
      781 ccgttgagcg gttgcagcgt gtggaagtag agaggcagcg cgttggcgcc caggcgatag
      841 gggtaataga gcatatccgt gtagaacgga ttgctatggc gctccagcag cgccgtcttg
      901 aaccaccaga ggtcccagcg gctgacatcg gcgtcggcag tgatgccgta gatgatgccg
      961 tgttgcaggt ccgttaccag cggccaggtc atgatcacgg tgaggaccgt gaagacggcc
     1021 accggccaga gcgcggccag gagtcgccgc cccagggggc gcgggggcgc ggcggcgtgc
     1081 gtcgccgtgc cggtcgcggt ttccggcgcc gggccaccca tttctgccgt ggtcgcggtc
     1141 cggccggctg ttctatttct catgcgcggt tgtctgctcc ataacttatc tgcacattat
     1201 acggtaggcg cggggcccat gccaagcaga ctccggcatg ccccggcctc cgccggcggg
     1261 cgaacgatac ttgacaccta tcgaactgac agagaaatat caacttagca agaactgggc
     1321 gatgcagtat tcaatccgtc aagaactaga tcctatttca atccggctgt gctacactac
     1381 aaggggacca gaattagccc ggccccgact cccttcagag aggcaccaat atgtttcagc
     1441 atctgcacaa gcccgcgccc atccggctca ttggcatgct cacgcttgtt tgcgctttcg
     1501 tgctgctggg cgcggggctg tggggtgccc tgaatccggc aacgcgcacg caggcggcgg
     1561 ccccgcatag cgcggcgcgc acggcgaaca accgcatcgt caccgagaac gcgctgccgg
     1621 gcacggacga gtgggccgac atcggctgga acgacccgcc caagctcaac gcctatgccg
     1681 gggcgaccag cgtgaacgcg ggcaacccga tcagcatcta cgtcaacaac gccggtagct
     1741 cgatcacctg gcggctgtac cggctgggct actaccaaaa ccacggtgcc cgcctgatca
     1801 ccacgggcgg accggtcgcc acctcgccgc agcccgcctg cacccgcacc agttcgaccg
     1861 gcctggtgcg ctgcccctgg gccgccgcct tcaccctgcc caccaatgcc gcctgggtca
     1921 gcggcatcta tctgctgcgc atggacagcg accaggggcg gcgcatctat gtctacttca
     1981 ccgtccgcaa cgacacctac aacgccgatt ttgtcttcca ggaagcgtcg atgaccaatc
     2041 aggcgtataa cgactacggc ggcgagagcc tctatctctc gcgcaataac gagggccgtc
     2101 cgcgcggcta ccaggtgtcc ttcgaccggc cctatgccga cggcgggggc accggttcct
     2161 tcttcaccca cgatgtcgat atgttacgct gggtcgaagc gtcgggctac gacgtgacct
     2221 atatcagcga tgtggacacg gccacgaacc cgaacatcct gctcaaccac aaggtcgtgc
     2281 tcgaagtggg tcaccccgaa tactggacct ggggcgagcg cgataacgtc gaaaacgcgc
     2341 tggccgccgg agtgaacatg gtttttgcct cggccaacga gacctactgg agtgtgcgcc
     2401 tggagtcgtc gccggtcggg ccgaaccgga ttatcacctg ctacaaagac cccgcgctcg
     2461 accccacaca caccgcgccc tataccaccg tcgagtttga cggccccatc ctgcaacgcc
     2521 ccgacaacat cctggtcggc gtcggctacc agtcgtacta tgacgatgcg cgctacaact
     2581 atccctgggt gatcagcgcc ccggccacgc gctggtactt cgactgcacc ggcctccagc
     2641 ccggcgacca ggtgaacaac atcatcggcg aggagtgggc cgcggtccac gacaacgggc
     2701 acaccccgcc gggcctcgac gtgatctcca gcggtacggt gatcaacgcg catggggtgg
     2761 ggctggccca gaactcggcc atttacacgg cggcaagcgg ggcgaaggtc ttcgccgcag
     2821 gcagtatcca ctttgcctgg ggcctgatcg accactccta ccccaaccag gtcttccagt
     2881 cctacgcgct ctccaacgac gccgaccatc gcgtcgagca gttcatggcg aatatcctcg
     2941 acaacttcgc gggctactgg gacgggtcgc cgcgcacgtg cggcggccag ggttactacg
     3001 atgtgggcac gcgggccacg cgcacgccca tgccccaggt gcccacggcc accggcaccc
     3061 cgcccaccgc cacgcgcacg cccacccgga cgccgacccc ggtgagcggc accgcaacca
     3121 acaccccggt cggcacgccg accaataccc ccacggccac gccgaccagc ccgcccgtgc
     3181 agggcgcgat cacgaccagc gatcccaccc aggccgggcg ctttgtgcgc gactggaccg
     3241 tcgccagttg cggcaccgtc aagacggccc cgcaactggc cgacagcacg gcccggcact
     3301 atgacgccta tcagtatgtg aacaataccg gcagttcgca gtgtgttacc gtgcggctga
     3361 ccaccgcctg caccggcccg aacgatattt tcagcgcggc ctacctgggc agcttcaccc
     3421 cggccaacat cgtcaccaac taccgctctg atatcggcgc cagtccgggg caactcggcg
     3481 gcagcagcag ctacggcttc gatgtgcccg cgggccagac ctttgtcgtc accgtgtacg
     3541 aggtcacggc gaacgccggc tgcggcagct atacgctggc cgtgaccggc ggcagcccgg
     3601 taggcggggc caccagcacg cccaccgcca ccgtagccgc gacccacacc ccgacggcca
     3661 cccgcacggc gaccgcgtcc gctacgccgg cgcgtaccgc taccgcgacc agcacggcga
     3721 cggccaccag cacgtcgctg ccgccgtccg cgacagccac ggcgatcccc tccgctacgt
     3781 ctacggccat cccgaccgga acggcgaccg atacgcccct gcccagcgca acggccacgc
     3841 gcacgcccag ccacacggct accgtgccgc ccacggcgac ggatacgccg gcggtaagtc
     3901 cgagcagcac cagcacggcg accgctatcg ttagcccgac acttacggcc accgctgtgc
     3961 cgcccacgcg cacggcgacc gctaccgtga cagccacggc cccgccgaac agcacggcca
     4021 cccgcacggc gaccagcacg cgcacggcga cgcgcacgcc gacgagtacg cgcacgacca
     4081 cgcccacccg taccgctaca cgcacgccga tcagcacggc gacccgcacg ccgaccggca
     4141 tcgcgacgag caccgcgaca cgcacgccga tcaacacagg cacggccacg ccgaccgtca
     4201 ccgcgacgcc cggcggtggg ccgcagccga ttcagggcgc gatcaccagc ggcgacccga
     4261 cgcaggccgg gcgcatcgtg cgcgactggg tgccgagcac gtgcagcacc gtcaagaccg
     4321 tgcccggcaa cggcgatatg ctggcccacc actacgacgc ctacaccttc gccaacaata
     4381 ccgcgggcac cgcgtgcgtc accgtgcagc tcacgtcgcc ctgcacggcc ggcaacgaca
     4441 tcttcagcat cgcctacctg gacagcttcg acccggccaa tatcaccgcg aactaccgcg
     4501 gcgacatcgg gtggagtccc ggccaggaca ccggcagcaa cagctactcc ttcaccgtgg
     4561 cggcgggccg gcactttgtc gtgaccgtat acgaggtcac ggcgaacggc ggctgcggca
     4621 gctatacgct gaatg
//
LOCUS       DASUYC010000680         2633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1808305, whole genome shotgun sequence.
ACCESSION   DASUYC010000680 DASUYC010000000
VERSION     DASUYC010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1808305"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="VF276_11120"
     CDS             complement(<1..420)
                     /locus_tag="VF276_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607454.1"
                     /translation="MPTKRHKHTDLFLLRVWCDEEKGDEGSGLRWRGRLQRPTSGEAR
                     NFADSAALIAVLEAMLDERGPDQAEGSRKVPIDVQIRIAERVKERNVMILIRIVFQAK
                     FGKASELVEGMKQVLDYMKQGEQRARLLTDLSGPFDTV"
     gene            601..>2633
                     /locus_tag="VF276_11125"
     CDS             601..>2633
                     /locus_tag="VF276_11125"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6607455.1"
                     /translation="MDVSFGNWLKQRRQELGLTQDKLAGQIGCAPVTLQKIERGARHP
                     SRQVALLLAESLGVPAEEREAFTFFARGQLAGTIPEGLAATAIEPKGRSPWRVAGQRQ
                     TNLPALLTALIGREREEQALADLLAQPKVRLLTLTGPPGVGKTRLGLQVAAGQVAHFP
                     DGVFLVELAAVAEPDLVVPTIARTLGLVEQPGLPIDASVRAYLRQKRLLLLLDNFEQV
                     LDAAPEVVRLLQAGPALKVLVTSREALHVRGERRFRVPSLALPPRNTMGDLATLALNS
                     AVTLFVERAQDAQPAFVLTAANAADVAAVCVGLDGLPLALELAAAWTTLLSARQLREA
                     LTQRLQLVTRGARDLPVRQRTLREAIAWSYALLNAGEAQLFARLAVFQGGLTLEAGVA
                     VCNQDGVLALDPREGVQALLDKSLVQQGIGADGEPRFWMLETIREYAGEMLAAQGGAT
                     ALRTAHLVYFTRLAEQVEPELWGPQQYEGLARLEAEHDNLRAALHWAGQAGAPGAALG
                     LRLAGALGPFWHMRGYWSEGRIHLTAALAAPGLDTERAGRVRALLWAGLLATGQNAYA
                     DARSLLQQRLTLSRELGDPPGIAVALDGLVDVFLGQGDYPAAKTLAEQSLLLWREIGD
                     QGGIAYALSNLGLVAYEEGQYDAARARHEEGLEIVRALGDKRSIAHTLNNLGNL"
BASE COUNT          411 a          855 c          920 g          447 t
ORIGIN      
        1 gaccgtgtca aacggcccgc tcaggtccgt caagaggcgc gcccgctgct cgccttgctt
       61 catatagtcg aggacttgct tcatcccctc aaccagctca ctggccttgc caaacttggc
      121 ctgaaagaca atccgtatga gaatcatgac gtttctctcc tttactcttt ccgctatacg
      181 aatctgcaca tcaatgggaa ctttgcgcga cccttcagct tgatcagggc cgcgttcgtc
      241 gagcatggcc tccagcaccg ctatgagggc cgccgaatcc gcgaagttgc gcgcttcacc
      301 actggtcggc cgctgcagcc tgccgcgcca gcgcaaaccc gatccttcgt cccccttttc
      361 ctcgtcgcac cacacccgca acaggaaaag gtcggtgtgc ttgtgtctct tggtcggcat
      421 cggcaagttc ctctcaccac cagtataagg gcaagtcgtg cggagtggtc gatacaaaag
      481 tcgtacacaa gtcatacaaa atgcgctata ctggtgcggc agacaaccgc caccctgcgg
      541 cgagcgcgcc ctctgcggtt acggcgcgcc gggactccgc cagcgttgat gagggatcgt
      601 gtggacgttt ccttcggtaa ctggctgaag cagcgccgcc aagagctggg cctcacccag
      661 gacaagctgg ccggccagat cggctgtgcg ccggtgacct tgcagaaaat cgagcgcggc
      721 gcgcgccacc cctcccgcca ggtggccctg ttgctggcgg agtctctcgg cgtgcccgct
      781 gaggagcggg aagcgttcac cttcttcgcc cgaggtcagt tagccggtac catcccagag
      841 ggcctggctg ctaccgcgat tgagccgaag gggcggtccc cctggcgggt tgccggccag
      901 cgccaaacga acctccccgc gctgctgaca gcgctcattg gccgcgaaag ggaagaacag
      961 gcgctggccg atctgctcgc ccagccgaag gtacgcctgc tcaccctgac gggcccgccc
     1021 ggcgtcggta agacccgcct cggcctgcag gtggccgcgg ggcaagtggc gcacttcccg
     1081 gacggcgtgt ttctcgtcga gctggctgcg gtagccgagc cggatttagt cgtgcccacc
     1141 atcgcgcgca ccctgggtct ggtggaacag cccggcctcc cgatagacgc aagcgtgcgg
     1201 gcctacctgc ggcagaagcg gctgctcttg ctactggaca atttcgagca ggtgctcgac
     1261 gccgcgcccg aggtggtacg gctgctacag gccggccccg cgttgaaagt gctggtcacc
     1321 agccgcgagg cgctgcacgt ccgcggcgag cggcgcttcc gggtgccctc actggcccta
     1381 ccgccgcgca atacgatggg cgatctcgcc acattggccc tcaattccgc cgtgacgctc
     1441 tttgtggagc gcgcgcagga cgctcagccc gctttcgtgt tgacggcagc gaatgcggcg
     1501 gatgtggctg cggtctgcgt cgggctggat ggcctgccgc tcgccttgga gctggccgcc
     1561 gcctggacca ccctgctgtc ggcgcgacag ttgcgggagg ccctcaccca acgcctccaa
     1621 ttggtgaccc gtggggcgcg cgacctgcct gtccgccagc ggaccttgcg ggaggcgatc
     1681 gcgtggagct acgcgctgct caacgcgggg gaagcgcagt tgtttgcccg cctggccgtg
     1741 tttcagggcg gcctgacgct ggaggcgggt gtggcggtgt gcaaccagga cggggtactg
     1801 gccctggatc cccgggaagg ggtgcaagcg ctgctggaca agagcctggt gcagcagggg
     1861 atcggcgcgg atggggagcc gcgcttctgg atgctggaga cgatccgtga atatgccggc
     1921 gagatgctgg cggcgcaggg gggggcgacg gcgctgcgga ccgcgcacct ggtctacttc
     1981 accagactgg ccgagcaggt ggagccggag ttgtggggcc cgcagcagta tgaggggctg
     2041 gcgcggttgg aggcggagca cgacaatctg cgcgcggcgc tgcactgggc ggggcaggcg
     2101 ggtgccccgg gggcggccct cggcctgcgc ttggccgggg cgttggggcc gttctggcat
     2161 atgcgcggct actggagcga ggggcgaatc cacctgacgg cggcgctggc ggcacccggg
     2221 ctggatacag agcgcgccgg gcgtgtccgc gcgctccttt gggccgggct cctggcgacc
     2281 gggcagaatg cctatgccga cgcccggtcg ctgctccagc agcgcctgac gctgtcccgc
     2341 gaactggggg atccgcccgg tatcgccgtc gccctggatg gactggtgga cgtgttcctg
     2401 ggacaggggg actacccggc cgcgaagact ctcgcggagc agagcctgct gctgtggcgg
     2461 gagatcgggg accagggtgg catcgcctac gcactcagca acctggggct ggtggcgtat
     2521 gaggaagggc agtatgacgc ggcccgtgcc cggcacgagg agggtctgga aatcgtgcgc
     2581 gcactgggag acaagcggag catcgcccat accctcaaca acctggggaa tct
//
LOCUS       DASUYC010000681         6087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_540690,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000681 DASUYC010000000
VERSION     DASUYC010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6087
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540690"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3185
                     /gene="rpoC"
                     /locus_tag="VF276_11130"
     CDS             <1..3185
                     /gene="rpoC"
                     /locus_tag="VF276_11130"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX6607456.1"
                     /translation="DLNDLYRRVINRNNRLRKLLELSAPEIIIRNEKRMLQEACDALI
                     DNGRRGRAIAGTGNHRLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPDLKLHQ
                     CGLPKKMALELFKPFVMRRLVQQGLAHNIKSAKRIVERVRPEVWDVLEDVIKDYLVLL
                     NRAPSLHRLSIQAFEAVLIEGSAIQLHPMVCSAFNADFDGDQMAVHVPLSAAAQREAR
                     ELMLSVHNILSPAHGEPMVSPSQDIVLGCYYLTVMRTDGRTKGHGKVFANPAEAVLAY
                     HSGVVDLQAPVMVRMSLDGGKPQLVETTPGRIIFNEVIGRYNDQVGPEQQIAYRNVAL
                     DKGALRGVIGECHRKLGAARTAEIADNMKRLGFTYATKSGMSVAVADVEIPAAKDQML
                     ADADAEVDQIEREYRRGLLTDNERYQQVIEVWNRTTDEMTDELKKILDPYGLVNTLAS
                     SGATKARYKQIRQLAAMRGLMADPSGRIIELPIRSNFREGLTVLEYFVSTHGGRKGLA
                     DTALRTADAGYLTRRLVDVAQDVLITMEDCGTTAVSTIVEPPNTDVAESYAARVVGRF
                     TAQPVVDPKTGEIIVDANEEVTDVIMQRIEEAGIKSLPMRTPLTCDAQHGICQLCYGR
                     SLATGEIIAIGEAVGIIAAQSIGEPGTQLTLRTFHTGGVASSEDITQGLPRVEELFEA
                     RVPKGQAILAEIDGKIELTREDEIRRIKIVSSEIFNDPYQLPEGGHYELLVRPEQDVA
                     ENTPLFRSTLAGEEDNVTVAHTAGKVLIDEENNQLILRSEERDEREYQVLAGSRLTVE
                     DGDYVTAGTPLTEGSQNPQEILAIMGREAVQRYLVDQVQTVYRLQGVNVHDKHIEAIV
                     RQMLMKVRIDLPGDTDLLPGELIDRFKFEETNARVLAEGGEPATATPVLLGITKASLS
                     TDSFLSAASFQETTRVLTEAAINGQIDRLRGLKENVIIGKLIPAGSGATRRPQLGDLT
                     MVDEGTNIDDYLRISQGGDEEIPADATGSGGEDEDSVVSLETAGADPEFALSELLGQS
                     VLVGGDSDSDDPTEAEAILIDQDNLDEPAD"
     gene            3438..3653
                     /locus_tag="VF276_11135"
     CDS             3438..3653
                     /locus_tag="VF276_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012969873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX6607457.1"
                     /translation="MKTYLVVFEPADHNWAAYAPDVPGCIATGRTREEVERTFVEALA
                     FHLEGLRLEGLPIPQPVAEAGHVAVPA"
     gene            3924..4370
                     /gene="rpsL"
                     /locus_tag="VF276_11140"
     CDS             3924..4370
                     /gene="rpsL"
                     /locus_tag="VF276_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX6607458.1"
                     /translation="MPTINQLVRKGRQSATKKSKAPALRFRFNAFSQTTQRVQGSPQM
                     RGVCTNVRTQTPKKPNSALRKVARVRLSNSMEVTAYIPGEGHNLQEHSVVLIRGGRVK
                     DLPGVRYHIIRGVLDTQGVKDRKKGRSKYGQKSAANIKPGAKGGKR"
     gene            4729..5199
                     /gene="rpsG"
                     /locus_tag="VF276_11145"
     CDS             4729..5199
                     /gene="rpsG"
                     /locus_tag="VF276_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX6607459.1"
                     /translation="MPRRAHYEKRDPGLDAKFNHPVVARFINKIMIGGKKGVAEHIMY
                     DALDIIERNTHRPAIEVFEVAMRNATPVLEVKPRRVGGANYQVPVEIKGDRRVALAIR
                     WILRAARARNGKSMADKLAAELIDASNNVGATIKRREDTHKMAEANRAFAHYKW"
     gene            5237..>6087
                     /locus_tag="VF276_11150"
     CDS             5237..>6087
                     /locus_tag="VF276_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019647170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX6607460.1"
                     /translation="MPRAFPLEKIRNIGIIAHIDAGKTTTTERVLYYTGRIYKIGEVH
                     EGAATMDWMEQERERGITITASATTAQWKDCRINIIDSPGHVDFTVEVERSLRVLDGG
                     VVVFDSVAGVEPQSETVWRQADKYGVPRICFINKMDRTGANFWRTVDMIRERLNATPV
                     CIQLPIGSEANFQGIIDLIANKAIIYTDDLGTTHSETDIPADMAEEVAAQREKMIESR
                     LGATPLVTQIPIGTESDFIGVVDLVNNRAIIWLEESLGAKFEYQDIPDDLKDKAAAAR
                     EKLIELAV"
BASE COUNT         1200 a         1931 c         1968 g          988 t
ORIGIN      
        1 ccgacctgaa cgatctctat cgccgggtca tcaaccgcaa taaccgcctg cgcaagctgc
       61 tggaactctc ggcgccggag atcatcatcc gcaacgagaa gcgcatgctg caggaagcct
      121 gtgacgcgct gatcgacaac ggccgccgcg ggcgtgccat tgccggcacc ggcaaccatc
      181 ggctcaagag cctgagcgac atgctcaagg gcaagcaggg ccggttccgc cagaacctgc
      241 tgggcaagcg cgtggactac tcgggccgct cggtgatcgt ggtcggcccg gacctgaagc
      301 tccaccagtg cggcctgccc aagaagatgg cgctggagtt gttcaagccg ttcgtgatgc
      361 gccgcctggt gcagcagggg ctggctcaca acatcaagag cgccaagcgc atcgtcgagc
      421 gggtgcgccc cgaagtttgg gacgtactcg aagatgtcat caaggactac ctggtgctgc
      481 tgaaccgcgc gccgtcgctg caccgcctgt cgatccaggc gttcgaggcc gtgctcatcg
      541 aaggctcggc tatccagttg cacccgatgg tctgctcggc cttcaacgcc gacttcgacg
      601 gcgaccagat ggccgtgcac gtgccgttgt ccgccgctgc ccagcgcgag gcccgcgagt
      661 tgatgctctc ggtgcacaat atcctctcgc cggcgcacgg tgagccgatg gtcagcccgt
      721 cgcaggacat cgtcctgggc tgctactatc tgacggtgat gcgcaccgac ggccgcacca
      781 agggccacgg caaggtcttc gccaacccgg cggaggcggt gctggcctat cacagtggcg
      841 tggtggacct gcaggcgccg gtcatggtgc gcatgtcgct cgacggcggc aagccgcagt
      901 tggtcgagac gacgcccggc cgcatcatct tcaacgaggt gatcgggcgc tacaacgatc
      961 aggttggccc ggagcagcag atcgcctacc gcaatgtcgc gctcgataag ggcgcgctgc
     1021 gcggtgtgat cggcgagtgc caccgcaagt tgggcgctgc ccgcacggcg gaaatcgccg
     1081 acaacatgaa gcgcctgggc ttcacctacg ccaccaagtc cggcatgtcc gtggccgtgg
     1141 ccgatgtgga gatcccggcg gccaaggacc agatgctggc cgacgccgat gccgaagtcg
     1201 atcagatcga gcgcgagtat cgccgcggtc tgctcaccga caacgagcgc taccagcagg
     1261 tcatcgaggt ctggaaccgg accaccgatg agatgaccga cgaactcaag aagatcctgg
     1321 acccgtacgg cctggtgaac acgctggcta gctcgggcgc gacgaaggcg cgttacaagc
     1381 agatccgcca gttggccgcc atgcgcggcc tcatggccga cccgtccggg cgcatcatcg
     1441 agctgcccat tcgctcgaac ttccgcgaag ggctgacggt gctggagtac ttcgtgtcca
     1501 cccacggtgg gcgcaagggt ctggccgata ccgcgctgcg cacggcggac gccgggtatc
     1561 tcacccgccg cctggtcgat gtggcgcagg acgtcctgat cacgatggag gactgcggca
     1621 ccaccgcagt gtcgaccatc gtcgaaccgc caaacaccga tgtggccgag agctacgccg
     1681 ctcgcgtggt gggccgcttc acggcgcagc cggtggtcga tcccaagacg ggtgaaatca
     1741 tcgtcgatgc caacgaggaa gtcaccgatg tgattatgca gcggatcgag gaggcgggca
     1801 tcaagtccct gccgatgcgc acgccgctga cgtgcgatgc ccagcacggc atctgccagc
     1861 tttgctacgg tcgctcgctg gcgaccggcg agatcatcgc catcggcgag gcggtcggca
     1921 tcatcgccgc ccagtccatc ggcgagccag gtacgcagct tacgttgcgc acgttccaca
     1981 ccggtggtgt ggcttcgtcc gaagacatca cccagggtct cccgcgcgtc gaggagctct
     2041 tcgaggcgcg cgtgcccaag ggccaggcca tcctggcgga aatcgacggc aagatcgagc
     2101 tgacgcgcga ggacgagatt cgccgcatca agatcgtgtc gagcgagatc ttcaacgatc
     2161 cgtaccagtt gccggagggc gggcactatg aactgctcgt ccgccccgag caggacgtgg
     2221 cggagaatac gccgctcttc cgctcgaccc tggcgggcga ggaggacaac gtcaccgtcg
     2281 cccacacggc gggcaaggtg ctgatcgatg aggagaacaa ccagcttatc ctgcgcagcg
     2341 aggagcgcga cgagcgcgaa taccaggtgc tggccgggtc ccggctcacg gtggaagacg
     2401 gcgactatgt caccgccggc acgccgctga ccgaaggctc gcagaacccg caggaaatcc
     2461 tggcgatcat gggccgcgag gccgtgcagc gttacctggt ggaccaggtg cagacagtgt
     2521 accgcctgca gggtgtgaat gtccatgaca agcacatcga ggccatcgtg cggcagatgc
     2581 tcatgaaagt gcgcatcgac ctgccgggcg ataccgacct gctgccgggc gagttgatcg
     2641 atcgcttcaa gttcgaggag accaacgccc gcgtgctggc cgaaggcggc gagccggcga
     2701 cggccacgcc cgtcctgctc ggcatcacca aggccagcct gagcaccgac agcttcctgt
     2761 cggcggcctc cttccaggag accacgcgcg tgctgaccga ggcggccatc aacggccaga
     2821 tcgaccggct gcgcggcctg aaggagaacg ttatcatcgg caagctgatc cccgccggca
     2881 gcggggccac ccgccgcccg cagctcggcg acctgaccat ggtggacgag gggaccaaca
     2941 tcgacgacta cctgcgcatc agccagggcg gcgacgagga gatccccgcc gacgccaccg
     3001 ggtcgggcgg cgaggacgag gacagcgtgg tttccctgga aaccgccggg gccgatccgg
     3061 agttcgcgct cagcgaactc ctcggccagt cggtcctcgt cggcggcgac agcgacagcg
     3121 acgacccgac ggaagccgag gccatcctga tcgatcagga caacctcgac gagcccgccg
     3181 actaatcgcg aatggcctgc taagcaagag agccgccggt gttctgccgg cggctctttt
     3241 cgccaatgta tagccggttg tccggcacgg gccggtttca aaccggcccc tacaccaaat
     3301 aggtcgggcc ggtttcaaac cggctcctac atatcttgat ccttgtcagg ggtccgtagc
     3361 aaagaggcga tcgtgtaggg gtcggtttca aaccgaccca gcggtctctt actgagacgg
     3421 gctaccaggg ggaagccatg aagacgtatc ttgtcgtctt tgagccggct gaccataact
     3481 gggccgccta tgcgcccgat gtgccgggct gtatcgcaac cggccggacg cgcgaagaag
     3541 tcgagcgcac tttcgtggag gccttggcct ttcacctgga gggactgcga ctggaaggac
     3601 tcccgattcc gcagccggtg gccgaagccg ggcatgtagc cgtacccgcc tgaagaggct
     3661 gcttccaacg ttgcggcgct ggcgcaagct tgacgcctct tctgctgtca ggtataatat
     3721 atgctgggct gtcgcgcccc ctcccggcgg ttgatcaggc aacccacagg ttccgacatt
     3781 tatatgcagc catcgtgccg tgtgccggcg ttgctgggcg gatggccggt ggctgaccgc
     3841 gaaccgcgaa tcttcgcttg acacatgcct gggcgtgtgt tataatctta aactgtgctc
     3901 atttagagac tctaggagga acaatgccga caatcaacca gcttgtgcgt aagggccggc
     3961 agagcgcgac gaagaagagc aaggcgcccg cgctgcgttt ccgcttcaat gcgtttagcc
     4021 agacgacgca gcgtgtccag gggtccccgc agatgcgcgg ggtctgcacc aacgtgcgca
     4081 cccagacgcc caagaagccg aactccgcgc tgcgcaaggt cgcccgcgtg cgcctgagca
     4141 actcgatgga agtcaccgcc tacattcccg gcgagggcca caacctgcag gagcactcgg
     4201 tggtgctgat ccgcggcggt cgcgtgaagg acctgcccgg tgtgcgctac cacatcatcc
     4261 gcggcgtgtt ggacacccag ggcgtgaagg accggaaaaa gggccgctcg aagtacgggc
     4321 agaagtccgc ggcgaatatc aagccggggg ccaagggcgg caagcggtag tctggcctcg
     4381 ggccagtttg tttcagtggc gaactcgcct gccgagtttg cccgcaggtg aagccggcgc
     4441 gctctgcgga gcgcccgctt cttgagacac gggtggcacc tgcggtcgcc cagtgggatg
     4501 tgaaggccgg ctccggcgcg cccttttagc cggagccgta gcaaatacaa agatagcgct
     4561 aatattaacc acaacctaat ccagctaagt ataacgcagg gccgcggtcc tgtggttacg
     4621 gatggtcgac ttagcagaga tggaaatggg gaggacgcct cggtagtcac gggggcgacc
     4681 tcgtttccat ctttctgtgt gttttacagc ataaggagat ccaagaccat gcctcgtcgg
     4741 gcacactacg aaaagcgcga tccgggactc gacgccaaat tcaaccaccc ggtggtggcg
     4801 cgcttcatca acaagatcat gattggtggc aagaaggggg tggccgagca catcatgtat
     4861 gatgcgctgg acattatcga gcgcaacacc catcgcccgg ccatcgaagt gttcgaagtc
     4921 gccatgcgca acgcgacccc ggtgctcgaa gtgaagcccc ggcgcgtcgg cggcgccaac
     4981 taccaggtgc cggtggaaat caagggcgac cggcgcgtgg ccctggcgat ccgctggatc
     5041 ctgcgtgccg cccgcgcccg caacggcaag agcatggcgg ataagctggc ggccgaactg
     5101 atcgacgcca gcaacaacgt tggggcgacg attaagcgcc gggaagatac gcataaaatg
     5161 gctgaagcca accgtgcctt tgcccattat aagtggtaag gggctagttg ctcgaccctg
     5221 actgggagtt aaaaacgtgc caagagcttt tccattagaa aagatccgta atatcgggat
     5281 catcgcccat atcgacgccg gtaagaccac gaccaccgag cgcgtgctct attacaccgg
     5341 gcggatttat aagatcggcg aggtccacga aggcgcggcg accatggact ggatggagca
     5401 ggagcgcgag cgcgggatca ccattaccgc ctccgccacc accgcccagt ggaaggattg
     5461 ccgcatcaat atcatcgaca gccccggcca cgtggacttc accgtcgagg tggagcgctc
     5521 gctgcgcgtc ctcgacggcg gcgtcgtcgt gttcgactcg gtggccggcg tggagccgca
     5581 atcggagacg gtgtggcgcc aggccgataa gtacggggtg ccgcgcatct gcttcatcaa
     5641 caagatggac cgcaccggcg cgaatttctg gcgcacggtc gatatgatcc gtgagcgcct
     5701 caatgcgacc ccggtctgca tccagttgcc gattggttcc gaggccaact tccagggtat
     5761 catcgacctg atcgctaata aggcgatcat ctacaccgac gacctgggca ccacgcacag
     5821 cgagaccgac atccccgccg acatggccga ggaagtcgct gcccagcgcg agaagatgat
     5881 cgagagccgc ctcggcgcca ccccgctggt cacccagatc ccgatcggca ccgagtccga
     5941 cttcatcggc gtggtcgacc tggtgaacaa ccgcgcgatc atctggctcg aggagagcct
     6001 gggcgcgaag ttcgaatatc aggacatccc ggatgacctg aaggacaagg ccgccgcggc
     6061 ccgcgagaag ctcatcgagc tcgccgt
//
LOCUS       DASUYC010000682         4808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_335202,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000682 DASUYC010000000
VERSION     DASUYC010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335202"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..623
                     /locus_tag="VF276_11155"
     CDS             159..623
                     /locus_tag="VF276_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607461.1"
                     /translation="MEEHDQAGAEGHLQGVVRADKVALRNGVAVAIVGSNEAQVASGG
                     ALVIAAGNTMQVNGGGAGALMAGNTLEIHGGGGWVLLAGNSIPIQNGGGAVLVAGRAE
                     VKHGVVGVLLAGHTTLGEGARVLLSTPQALALGAACGLVFALVGRGRRGRAR"
     gene            655..2280
                     /locus_tag="VF276_11160"
     CDS             655..2280
                     /locus_tag="VF276_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019046220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6607462.1"
                     /translation="MDYDAIIIGGGHNGLVAACYLAQAGRRVLVCDRRPRPGGAVNTE
                     ELFPGFRLDTCSTFHVLIHLTPILRELDLARFGLRYMRHDPVVFAPFPGGRSFVFWKD
                     LDATCRSIAAWSERDADAYGAFIRKWWHFNKAVFATFLAPPTPANLVGRMARQTMRDM
                     MAGRADPSMGGLELFRKAITTYGRLLDETFETPELKATLAWLAAQSGPPPSEVGAGDL
                     LGSLVIYHKVGLTRPVGGSGRLAEALVACLEHHGGTFQGGEAVRRVLVQGGRAAGVEL
                     ASGARVTAPVVVSNAHVQTLLLDLLPPDAVPADLRTKVAALRTGNGIGMTIRCAVDAL
                     PDYRAYLHDGAGQPSDPAVQIHAGMQLICPSVDYLQQAYEDAAQGRPAARPALVVMTP
                     SALDPTLAPPGKHVLYIWAQYHPYELRDGGAAAWDGIREREADRLLATLGEYAPNFPG
                     AVRDRQIYIQSPLDLERNAGFVHGNIMHLDMSLDQMFMFRPLPELSSYRTHLPGLYLT
                     GASTHPGGGVCGASGRSVAQVVLADLAKAGKRR"
     gene            2277..2960
                     /locus_tag="VF276_11165"
     CDS             2277..2960
                     /locus_tag="VF276_11165"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEX6607463.1"
                     /translation="MSAQRAILALAGAGVGVLALMLGVATWGWNFVAGAAFLVVLALG
                     LGISMARLRAMPVDPTNGSASRGRPAAAATLTGPVPGADPLASLQALTPAQFEEFARL
                     LLMASRLYHDVQVVGASGDQGIDLTMRDGAGNLCVVQCKRYKGTVSPRVVRELYGVMA
                     RTGAREAFLITTGRVSSGAQAWIGAKPVHVWDAERLLAYARRYLGPDLGQTIKRAQAA
                     STGRVSRGS"
     gene            complement(2955..3027)
                     /locus_tag="VF276_11170"
     tRNA            complement(2955..3027)
                     /locus_tag="VF276_11170"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2992..2994),aa:Thr,seq:cgt)
     gene            3237..3752
                     /locus_tag="VF276_11175"
     CDS             3237..3752
                     /locus_tag="VF276_11175"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX6607464.1"
                     /translation="MSQPVEEPALVELFRFNTWATLKLLDVCARLDDAQLNWTAPGTF
                     GSIRDTLTHLSGSEGGYVYQLTGERPSIPLKEDAFLGIPALRERARWFGTQLAQKAAT
                     VRRDDTFQSEWQGEIETVNCLTVLTQALVHGVDHRSQIATILSQHAIEPPDIDSWAYA
                     EEMKQAERQPA"
     gene            complement(3774..4616)
                     /locus_tag="VF276_11180"
     CDS             complement(3774..4616)
                     /locus_tag="VF276_11180"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6607465.1"
                     /translation="MADSQLHYEERGKGLPVVLLHGFPFDHTIWQAQLDGVGEVARVL
                     APDLPGFGTSPLPPPEPLIDDYVAALARWVDALDLDRFVLVGHSMGGYVALSFAGHYM
                     GRLAGLGLVCTRPGPDTEQARENRYSLINEVWDRGAEATVDRMLPNLLAPATQEKHPE
                     IGDQVRVVMQRQPPGGIVSALWAMARRPDSTPLLGNITVPTLVVSGADDRIIPAEDAD
                     LMAASIPGAVQERIPSAGHLPMLEQPDRLTDLLRDLARGSRTAPDIPEPPPSQDIAGV
                     RTQR"
BASE COUNT          777 a         1614 c         1652 g          765 t
ORIGIN      
        1 ttccgaagac agatgctcta gtccactgag ctacgcgcgc gggcaggcgc cactggtaga
       61 ttaccggatt cactctgcgg tgtcaagcga gggaacacgc ttccacggtg ccgcctatgc
      121 tataatgccc ccggcttacg cagaagggag gctagcccat ggaggagcac gatcaggcag
      181 gcgcggaagg ccacctgcag ggagtggtgc gcgcggataa ggtagcgttg cgcaatggcg
      241 tggccgtcgc catcgtgggc agcaacgagg cgcaggtagc gagcggcggg gcgctggtca
      301 tcgccgccgg gaacacgatg caggtcaatg gcggcggggc cggggcgttg atggccggga
      361 acaccctgga gattcacggc gggggcggtt gggtgctgct ggccggcaac agtataccca
      421 tccagaatgg gggcggcgcg gtgctggtcg cgggccgggc cgaagtgaag cacggggtgg
      481 tgggcgtgct gctggccggg cacacaacgc tcggcgaggg ggcgcgggtg ctgctgagca
      541 ccccccaggc actggcgctg ggcgcggcct gcgggctcgt atttgcgctc gtggggcggg
      601 gccggcgcgg gcgggcgcgc taagcagggc cgccgcgacc aaaggatcac cagcttggac
      661 tacgacgcca ttattatcgg cggcgggcat aacggcctgg tcgccgcgtg ctacctggcc
      721 caggccgggc ggcgcgtgct ggtctgcgac cggcggccgc gccccggcgg tgccgtcaac
      781 accgaggaac tcttccccgg cttccggctc gatacctgct ctacgttcca cgtgctgatc
      841 cacctcactc cgatcctgcg cgaactcgac ctggcccgct tcggcctgcg ctatatgcgc
      901 cacgacccgg tggtcttcgc gccgttcccc ggtgggcgat cgttcgtctt ctggaaggat
      961 ctcgacgcta cgtgccgctc gattgccgcg tggtcggagc gcgacgcgga tgcctatggc
     1021 gcgtttatcc gcaagtggtg gcatttcaac aaggccgtct tcgccacctt cctggcgccg
     1081 cctaccccgg ctaacctggt ggggcgcatg gcccggcaga ccatgcgcga tatgatggcc
     1141 gggcgcgcgg acccgagcat gggcggcctg gaactgttcc gcaaggcgat caccacgtat
     1201 gggcggctgc tggacgagac gttcgagaca cccgaactga aggccacgct ggcctggctg
     1261 gccgcgcaga gtgggccgcc gccctcggaa gtgggcgcgg gcgatctgct ggggtcgctg
     1321 gtgatctacc acaaggtggg gctgacacgg cccgtgggcg ggtcggggcg gctggccgaa
     1381 gcgctggtcg cctgcctgga gcaccacggc ggcactttcc agggcggcga ggccgtgcgg
     1441 cgcgtgctgg tgcagggcgg gcgggccgcc ggggtggaac tggcgagtgg cgcgcgggtc
     1501 acggcgccgg tggtcgtgtc gaacgcgcat gtccagacgc tgctactcga cctcctgccg
     1561 cccgatgccg tgcccgcgga tctgcggacc aaggtggcgg ccctgcgcac cggcaacggc
     1621 atcggcatga ccatccgctg cgccgtggac gccttgcccg actaccgcgc ctacctgcat
     1681 gacggcgccg gacaacccag tgacccggcg gtgcagatcc acgccgggat gcaactgatc
     1741 tgcccgtcgg tcgattatct gcagcaagcc tacgaggacg ccgcccaggg ccgccccgcc
     1801 gcccgcccgg cgctcgtcgt gatgacccct tcggcgctcg accccacgct ggcgccaccg
     1861 ggcaagcatg tgctctatat ctgggcgcaa tatcacccct atgaactgcg cgacggtggc
     1921 gcggcggcct gggacggcat ccgcgagcgg gaagccgacc ggctgctggc gaccctgggc
     1981 gaatacgcgc ccaatttccc cggcgcggtg cgcgaccggc aaatctatat ccagagtccg
     2041 ctcgacctgg agcgcaacgc cggcttcgtg catggtaaca tcatgcacct ggatatgagc
     2101 ctggatcaga tgttcatgtt ccgcccgctg ccggagctgt cgagctatcg cacccacctg
     2161 cccggcctct acctgaccgg ggcaagcacc caccccggcg gcggggtatg cggcgcctcg
     2221 gggcggtccg tcgcccaggt cgtgctggcc gacctggcga aagccggtaa gcggcgatga
     2281 gcgcgcaacg ggcgatcctg gccctggccg gcgcgggcgt gggcgttctc gcgctgatgc
     2341 tcggcgtcgc cacctggggc tggaatttcg tggcgggcgc ggccttcctg gttgtgctgg
     2401 ccctgggatt gggtatatca atggcccggc tgcgcgccat gccggtagat cctacgaacg
     2461 ggagcgcatc gcgcgggcgc ccggcggctg cggctacgct caccggcccc gtgcccggcg
     2521 ctgatccgct ggcgagcctg caagctttga ccccggccca gttcgaggag ttcgcgcgcc
     2581 tgctgctcat ggcgagcagg ctatatcacg atgtgcaggt ggtgggcgcg agcggcgacc
     2641 agggcatcga cctgacgatg cgcgacggtg caggcaatct ctgcgtggtc cagtgcaagc
     2701 gctacaaagg cacggtgtcg ccccgtgtgg tgcgcgaact atacggcgtg atggcgcgca
     2761 ccggcgcccg cgaggcattc ctgatcacca cgggccgcgt cagcagcgga gcccaggcgt
     2821 ggatcggcgc gaagccggtg catgtctggg atgcggagcg cctcctcgcc tacgcgcggc
     2881 gctatctggg cccagatctg gggcagacaa tcaaacgcgc ccaggcggca agcaccgggc
     2941 gcgtttcgcg agggagctga tgatgagact cgaactcatg acctgcgcat tacgagggcg
     3001 ctgctctacc aactgagcta catcagcata ttggatacgc ccatattata gaaaaatcgg
     3061 ccagggttgt caaggcgatt ccgcccggcg ccccgtggca tccgacgaac ggcgtattga
     3121 ctggaacaga tgttcggcgg taaaatggtg ctactgtccg ctcatagcga tcccgcacag
     3181 gcggcgcctg atgtgccggt gctgctggag tagcgaatgt atagggaggt cgcgctatgt
     3241 cccaaccggt ggaagaacct gcgctggtcg aactgttccg ctttaatacc tgggctactc
     3301 tcaagctatt ggatgtatgc gcgaggctgg acgacgccca actcaactgg accgcgcccg
     3361 gcaccttcgg cagcatccgc gatacgctca cccacctttc cgggtccgag ggcggctatg
     3421 tctatcagtt gaccggcgag cggccctcga tcccgctgaa ggaagacgca ttcctcggca
     3481 tccccgcgtt gcgcgagcgc gcccgctggt tcggcacgca actggcgcaa aaggcggcaa
     3541 ctgtgcgccg ggacgatacc ttccagagcg agtggcaggg ggagatcgag accgtgaact
     3601 gcctcaccgt cctcacccag gccctcgtcc atggggttga ccaccgcagc cagatcgcca
     3661 ccatcctgtc gcagcacgcc atcgaaccgc ccgacatcga ctcctgggcc tatgccgagg
     3721 agatgaagca ggcggaacgg caaccggcct gaccctggga tacccggccc gcgctagcgt
     3781 tgcgtccgca cccccgcaat atcttgcgac ggcggcggtt cagggatgtc cggcgccgtg
     3841 cggctgccgc gggccagatc gcgcaagaga tcggtcagcc tgtcgggttg ctccagcatg
     3901 ggcaagtgac ccgcgctggg gatgcgttct tgcacggcac ccggaatcga cgccgccatc
     3961 agatcggcgt cttccgcggg gatgatccgg tcatcggcgc cgctgaccac cagggtcggc
     4021 acggtaatgt tgcccagcag cggggtggag tcgggccgcc gggccatggc ccacagcgcc
     4081 gagacgatcc cccccggcgg ctggcgctgc ataaccacgc ggacctggtc cccgatctcc
     4141 gggtgcttct cctgcgtggc aggcgccagc aggttcggca acatgcgatc cacggtcgcc
     4201 tcggcgccgc ggtcccacac ttcgttgatc aggctatagc gattttccct ggcctgctcg
     4261 gtgtcggggc cgggccgggt gcagaccagg ccgaggcccg ccagccgccc catgtagtgg
     4321 ccggcgaagc taagggccac gtacccgccc atcgagtgcc ccaccagcac gaagcggtcc
     4381 aggtccagcg catccaccca gcgggccagc gcggcgacat agtcgtcgat cagcggctcg
     4441 ggcggcggca gcggggatgt gccgaacccc ggcagatcgg gcgccagcac acgggccacc
     4501 tcgcccaccc cgtctaattg cgcctgccag atggtgtgat cgaacgggaa cccatgcagc
     4561 agaacaacag gcaacccctt gccccgttcc tcatagtgta attgcgaatc agccatagct
     4621 aactacctcg ccaatcatag tgataatccg taaccccccg ccccggtcgc ttggccgcgt
     4681 ccctggcgcg cgggtcttgc tgatgcagac tccagcaaat acgagaccag cgctggccga
     4741 cgccacacca atccaccggc tgtatagcct gagcgacccc ggtttgaccg catccggcaa
     4801 tcccgcta
//
LOCUS       DASUYC010000683         3421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_84690,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000683 DASUYC010000000
VERSION     DASUYC010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84690"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            312..1448
                     /locus_tag="VF276_11185"
     CDS             312..1448
                     /locus_tag="VF276_11185"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX6607466.1"
                     /translation="MASKSPNIHKAYLPGMTADVAGGGEITLPLEVLARRLNRNPEDV
                     LRLDSDENPYGCSLRVMEALGSAESLHRPGDPEARDLRAALEPYALCARDRIAVGAGP
                     AESLERLLRVLTRPGQAIAVPAPTRPLYAGVAERLGREVVEIPLTRGFEVDTASLIAA
                     AETGQIALIIIAAPNDPTGTSITATEVVRLLRTEVPIVVDESLYEFSGRTVVPLVTEF
                     ENLVVLRDFAPWAGLGGLPISYTLSARPLAARLRHAAVFGGTTSAPGRAAQLAALASL
                     QDLDILRTHVKALRQERGRMFRSLRKLNLLQPYPSEANFLLCAMTRGNAAAIRDHLAD
                     NDGIIVRAIATPLLPNHLRISVGRPQDTEALMRGLLRIAEQHPL"
     gene            complement(1455..2192)
                     /gene="rph"
                     /locus_tag="VF276_11190"
     CDS             complement(1455..2192)
                     /gene="rph"
                     /locus_tag="VF276_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX6607467.1"
                     /translation="MPRPDGRRNDEMRPLRITPHFQSGPAGSVLIETGQTRVACAVSV
                     DLKVPPWLAGKGRGWLTAEYGMLPGSSPQRIARPINKPDGRATEIQRLIGRSLRAMVD
                     LDRLGELLLQLDCDVIEADGGTRTAAITGAAVAVVLALHRLRAAGTLPGPALPLRGLV
                     AAVSVGLVDGEPRLDLPYLEDSRAEVDCNLVLTSAGEWIEVQATAEHGAFSRAMLDEF
                     LRLGELGLTGLFAAQRAVLAAEGVSGL"
     gene            complement(2201..>3421)
                     /locus_tag="VF276_11195"
     CDS             complement(2201..>3421)
                     /locus_tag="VF276_11195"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEX6607468.1"
                     /translation="MPLVPVLAAMELAGVYVDVPQLQSLSTELYAKMAALEAQIFEAA
                     GHPFNINSSQQLGQVLFEELGLAGRSRTKTGYSTSQEVLDNIRHLHPIIDMVSEWRSM
                     NKLKSTYLDALPLLVNPDTGRVHTSFNQTVAATGRLSSSNPNLQNIPVRTEVGRLVRR
                     AFVADNTSPKPLVPRPSTLYAADYSQIELRILAHMTGEETLVNVFRSGGDVHAATAAE
                     LFDVPVDQVKPEMRYLAKRINFGVLYGMGTYGLTRDTSLTHQDAQGFLERYWARYPKI
                     RQFMDETLHEARKCGYVTTLLGRRRYMPELTSSNGGVRQAAERAAINAPVQGTAADII
                     KIAMIRLHRELRERKLAARMLLQVHDELLFELPEAELDEVDTLVRETMENAFPMDVPL
                     KVETKHGRSWGEME"
BASE COUNT          520 a         1163 c         1180 g          558 t
ORIGIN      
        1 gtcgcaagcc gtgctgggcg cgctgacgat cctggtgctg gtggcctggc cgcggctcac
       61 catccgcaga atgcccaggc tggacgcgcc ggccccgatg ggcgccgctt aggctctcca
      121 cacggttcgt agccgcgtaa aagcgcccgc gtcaatccgc acgcgggcgc ttttcgtatc
      181 ctggtacgtg gccttgacgg cggggtaccg ctttgatccg gggtctattg ctggtataat
      241 ggcagcagat caccggcggg ctgcgcaccg acccgccggt aagtgacgtg tagtgtccta
      301 gagagaatag cgtggcgagc aagagtccga atatccacaa agcctatcta cccggtatga
      361 ccgccgacgt ggccggcggc ggcgagatca ctctaccgct cgaagtgctg gcccggcggc
      421 tcaatcgcaa ccccgaggat gtgttacgcc tcgacagcga cgagaatccc tacggctgct
      481 cgttgcgcgt gatggaagcc ctgggcagcg ccgagtcgtt gcaccgcccc ggcgatcccg
      541 aagcgcgcga cctgcgcgcc gcgctggaac cctatgccct ttgcgcccgc gaccgcatcg
      601 ccgtgggggc cggtccggcg gagagcctgg agcgcctgct gcgcgtgctc acgcggcccg
      661 gccaggcaat tgccgtgccc gcgcccaccc ggcccctgta tgccggcgtg gccgagcgcc
      721 tgggacgcga ggtggtggag atccccctga cccgcggctt cgaggtagat acggcttcgc
      781 tgatcgccgc cgccgaaacg ggccagattg cgctgatcat catcgccgcg cccaacgacc
      841 cgaccggcac cagcatcaca gccacggagg tggtgcgcct gctacgcacc gaggtcccga
      901 tcgtggtgga cgagtcgctc tacgagttct ccgggcggac ggtggtgccg ctggtgacgg
      961 agttcgagaa cctggtggtg ctcagggatt tcgcgccgtg ggcgggcctg ggcggcctgc
     1021 cgattagcta caccttgagc gcgcggcccc tggcggcgcg gctgcgccac gcggcggtct
     1081 tcggcgggac gaccagcgcg ccaggccggg cggcccaact ggccgcgctc gcctcgttgc
     1141 aagatctcga catcctgcgc acgcatgtga aggcgctgcg ccaggagcgc gggcgcatgt
     1201 tccgcagcct gcgcaagctc aacctgctgc aaccctaccc gtccgaagcc aacttcctgc
     1261 tctgcgccat gactcgcggc aatgccgccg ccatccgcga ccacctcgcg gacaacgacg
     1321 gcatcatcgt gcgggcgatt gcgacgccgc tgctgcctaa ccacctgcgt atcagcgtgg
     1381 gccggcccca ggacaccgaa gccctgatgc gcggcctcct gcgcatcgcc gagcaacacc
     1441 cgctctaggg cgcgctagag tccgctgacc ccctcggcgg ccagcactgc gcgctgggcc
     1501 gcgaacagcc cggtcagacc caattcgccc aggcgcaaga actcgtccag catggcccgg
     1561 ctgaaggcgc cgtgctcggc ggtggcctgc acctcgatcc attccccagc gctggtcagg
     1621 accagattgc aatcaacctc ggcgcgcgag tcttccaggt aaggcagatc gagccggggt
     1681 tcgccgtcca ccagccccac gctgaccgcg gccacgagtc cgcgcagggg cagcgccggg
     1741 ccgggcagcg tgcccgccgc ccgcagccgg tgcagggcca gcaccaccgc caccgctgcc
     1801 ccggtgatgg cggcggtgcg cgtgccgccg tcggcctcga tcacgtcgca gtccagttgc
     1861 aacagcagtt cgccgaggcg atccaggtcg accatggcgc gcagcgagcg gccgatcagg
     1921 cgctggatct cggtggcgcg gccgtcgggt ttgttgatcg ggcgggcgat gcgctgcggc
     1981 gaggaaccgg gcagcatgcc gtactcggcg gtgagccagc cgcggccttt gccggccagc
     2041 cagggcggca ccttgagatc gacgctgacg gcgcaggcca cgcgggtctg gcccgtctcg
     2101 atcagcacgg agccggcggg gccgctctga aagtgcgggg tgatgcgcaa cgggcgcatc
     2161 tcgtcgttgc ggcggccatc gggtcggggc atggtgtgcg ctactccatc tcgccccagc
     2221 tacggccgtg cttggtctcg acctttagcg gcacgtccat ggggaaagcg ttctccatcg
     2281 tctcgcggac cagcgtgtcg acctcgtcaa gttccgcctc cggtagctcg aacaacagct
     2341 catcatggac ctgcaacagc atgcgggcgg ccagcttgcg ctcgcgcaac tcgcggtgca
     2401 ggcggatcat ggcgatcttg atgatgtcgg cggcggtgcc ctgcaccggg gcgttgatcg
     2461 ccgcgcgctc ggcggcctgg cgcacaccgc cgttcgacga ggttagttcc ggcatgtaac
     2521 gacggcggcc cagcagggtc gtgacgtagc cgcatttgcg ggcttcgtgc agcgtctcgt
     2581 ccatgaactg acggatcttc gggtagcgcg cccagtagcg ttcgaggaag ccctgcgcgt
     2641 cctggtgggt caggctggtg tcgcgggtca gcccgtaggt gcccatgccg tagagcacgc
     2701 cgaagttgat gcgcttggcg aggtagcgca tttcgggctt cacctggtcc accggcacgt
     2761 cgaacagttc cgccgcggtc gcggcatgca catcgccgcc gctgcggaag acgttgacca
     2821 gcgtttcctc gccggtcatg tgggccagga tgcgtaactc gatctgcgag tagtcggcgg
     2881 cgtagagcgt ggagggccgg ggcaccagcg gcttgggcga ggtgttgtcg gcgacgaaag
     2941 cgcgccgcac cagccgcccg acctcggtgc gcaccgggat gttttgcagg ttgggattgg
     3001 acgaactcag gcgcccggtc gcggcgacgg tctggttgaa cgaggtatgc acgcgcccgg
     3061 tgtcggggtt caccagcagc ggcagcgcgt ccaggtaggt cgatttgagc ttgttcatcg
     3121 agcgccactc gctcaccatg tcgatgatcg ggtgcagatg gcgaatgttg tcgaggacct
     3181 cctggctggt cgagtagccg gtcttggtgc gcgagcgccc ggccaagccc aactcctcga
     3241 acagcacctg ccccagttgc tgggacgagt tgatgttgaa cgggtggccc gccgcctcga
     3301 agatctgcgc ttccaacgcc gccatcttgg cgtagagttc ggtggatagg ctctgcaact
     3361 gcgggacatc gacatagacg ccggccagtt ccatcgcggc cagcaccggc accagcggca
     3421 t
//
LOCUS       DASUYC010000684         2349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_553273,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000684 DASUYC010000000
VERSION     DASUYC010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553273"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..1215
                     /locus_tag="VF276_11200"
     CDS             256..1215
                     /locus_tag="VF276_11200"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6607469.1"
                     /translation="MARTQTEPVARAASPLAAFGPELAQLLVISMWASTFVVTKAAFA
                     EVSPMAFIFVRFLLMTLLAFGVLAARGRKAQWRIRRADLPRFILAGLAGYTLYQLGFV
                     LGLDNTSPFSSSLLIAMVPFFTMLILAAMGERSPLGAWLGLGVGIVGVIIFLSDKQGG
                     AGTLLGDALSLGAAVSFAFYGVINRPLVRAYPTETYTAYAVGLGTIPLLILSTPAAVA
                     QPWGAITFWGWVAILYMVTLPVYVAYMLWNWAIARRGAAAATSFSLLVPIVSGTLSAI
                     FFSEDFGLLKLLGAACVLVGLVMLRSRAQKPAAPPAEGAPATE"
     gene            1329..>2349
                     /locus_tag="VF276_11205"
     CDS             1329..>2349
                     /locus_tag="VF276_11205"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6607470.1"
                     /translation="MINDESVRAVAAAHFPPQFVRPLYDSYCFSQIPQLVRHLLLGAP
                     DPGLPPSVLAGLPPAYDKVVLFFVDAFGWRFYERHAGNDPFLQRIAAEGVVSKLTAQF
                     PSTTAAHVTAIHTGLPVGESGVYEWFYYEPLVDALIAPLLFSFAGDRRRETLRQTGIP
                     PADFFPRTTLHQELRAGGVRSVIFQSREYTPSSYSDAVFAGAEVEPYRTLPEALARLA
                     HRLLTDAGRGYYFLYFDRIDTIGHFHGPDSAMFRAETDAFLAVMERVFHRTLAGALHN
                     TLFLLTADHGQVSGDPSATIYLNHLLPDLAADLLTNRAGVPLVPAGSARDMFLHVLPD
                     RLDAVQ"
BASE COUNT          323 a          810 c          782 g          434 t
ORIGIN      
        1 ctcctattct tagtaacgcc tgggcgccgg gaaaggtccg ggttcgggcg tctttattcg
       61 cttacaggtt gacaaggtat ggactggcgg cagggcttaa cgtgtgatga acgggcggct
      121 tacggcgcgg cccacctgtc attctgagcg cagcatcgat agcgaggtcc gaatccggcg
      181 cgcgaacgct cccattataa tgatataata ggcaggcagt aacgcatgcg cggcctgaag
      241 atgggagtga gattggtggc acgtactcag acggaaccgg tggcgcgggc ggcgtcgccc
      301 ctggcggcct ttggcccgga actggcccaa ctgctggtga tcagcatgtg ggcctcgacc
      361 tttgtcgtga ccaaggccgc cttcgccgaa gtcagcccga tggccttcat tttcgtgcgc
      421 ttcctgctga tgacgctcct ggccttcggc gtgctggcgg cgcgcgggcg caaggcgcag
      481 tggcgcatcc ggcgcgccga cctgccgcgc tttatcctgg ccgggctggc gggctacacg
      541 ctctaccagt tgggcttcgt gctgggcctc gacaacacct cgcccttctc cagctcgctg
      601 ctgatcgcca tggtgccgtt cttcaccatg ctgatcctgg cggcgatggg cgagcggtcg
      661 ccgctgggcg cctggctagg gctcggcgtc ggcatcgtgg gcgtgatcat cttcctgtcc
      721 gacaagcagg gcggggcagg cacgctgctg ggcgacgcgc tgagcctggg cgcggcggta
      781 tcgttcgcct tctacggcgt gatcaaccgc ccgctggtgc gcgcgtaccc gaccgaaacc
      841 tacaccgcct acgcggtggg cctgggcacc atcccgctgc tgatcctctc gacgcccgcc
      901 gccgtggccc aaccgtgggg cgcgatcacc ttctggggct gggtggcgat cctctacatg
      961 gtgacgctgc cggtctacgt ggcctacatg ctctggaact gggccatcgc gcggcggggc
     1021 gcggcggcgg ccaccagttt cagcctgctg gtgccgattg tcagcggcac gctctcggct
     1081 atcttcttca gtgaggactt cgggctgctc aaactgctgg gcgcggcctg cgtgctggtc
     1141 gggctggtga tgctgcgcag ccgggcacag aagcccgcag cgccccccgc ggagggtgcg
     1201 ccggcgacag agtagcggat ggcccggcgg tttcgttgtc atgctgaacg gagtgaagca
     1261 tctcgtcttg agtgcgcggg agacgagact cttcgctgcg ctcagagtga cacgggtggg
     1321 aggggcgaat gatcaacgac gagtcggtgc gggcggtggc ggcggcgcac tttccgccgc
     1381 agtttgtgcg gcccctgtat gacagctatt gcttctcgca gatcccgcag ttggtgcgcc
     1441 acctgctgct gggcgcgccc gaccccggcc tgccgccgag cgtgctggcg ggcctgccgc
     1501 ccgcgtatga caaagtggtg ctgttcttcg tcgacgcctt cggctggcgc ttctacgagc
     1561 gccacgcggg caacgacccg ttcctgcaac gtatcgccgc cgagggtgtg gtctccaagc
     1621 tgaccgcgca gttcccctcg accacggcgg cccatgtcac tgccatccat accggcctgc
     1681 cggtgggcga gagcggcgtc tacgagtggt tctattacga gccgctggtg gacgcgctga
     1741 tcgccccgct gctcttttcg ttcgccgggg accggcggcg cgagacgctg cggcagacgg
     1801 gcatcccgcc cgccgacttc ttcccgcgca cgacgctgca ccaggaactg cgggccgggg
     1861 gcgtgcgctc ggtcatcttc cagagccgcg agtatacccc gtcgtcgtac agcgatgccg
     1921 tgtttgccgg ggcagaggtg gaaccctacc gcacgctgcc cgaagccctg gcccggctgg
     1981 cccaccgcct gctgaccgac gcgggccgcg gctactattt cctctacttc gaccgcatcg
     2041 acaccatcgg ccacttccac ggccccgact cggccatgtt ccgcgccgaa accgacgcct
     2101 tcctggccgt gatggaacgg gtcttccacc gcacgctcgc gggcgccctg cacaacacct
     2161 tgttcctgct gaccgccgac cacggccagg tcagcggcga tccctccgcc acgatctacc
     2221 tcaaccacct cctgcccgac ctggccgccg acctgctgac caaccgcgcg ggcgtgcccc
     2281 tggtgcccgc cggctcggcc cgtgacatgt tcctgcacgt cctacccgac cggctggacg
     2341 cggtgcagc
//
LOCUS       DASUYC010000685         3020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1952524, whole genome shotgun sequence.
ACCESSION   DASUYC010000685 DASUYC010000000
VERSION     DASUYC010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1952524"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..289
                     /locus_tag="VF276_11210"
     CDS             137..289
                     /locus_tag="VF276_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005540900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEX6607471.1"
                     /translation="MRKLNDLRKQGQGLVEYALILVLIAIVVIVILAVLGGAVNDIFS
                     TIASAL"
     gene            375..812
                     /locus_tag="VF276_11215"
     CDS             375..812
                     /locus_tag="VF276_11215"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX6607472.1"
                     /translation="MLSTNWKPLHHVATSSGRRARQGTALLELALVMPILAALLLGVL
                     DFGRMYAEQLAVTSAAREGARAAMHDTSDTNVRNVVTNELNGAVVPNSSGSSCGNAIC
                     INRDTVNGQVVVTVNYTHSFLFGLIRNGDLALAASATMPYTAP"
     gene            836..1270
                     /locus_tag="VF276_11220"
     CDS             836..1270
                     /locus_tag="VF276_11220"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX6607473.1"
                     /translation="MNLSRLSPRATAYRRAPAQSMVEFALVAMVLFLMLFGIFEVSRL
                     VFTINSVNNGAREGSHYAALHPSATANDVKQAISPTLFLINVNDASNFSLSVVTDTTC
                     TAPCSYSPVTVRISNTLHLAVPFPGVGSSVVITSASTALTER"
     gene            1300..2799
                     /locus_tag="VF276_11225"
     CDS             1300..2799
                     /locus_tag="VF276_11225"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEX6607474.1"
                     /translation="MEWARFRKGQTVIIAALCLLALVAFVGLAVDGGSTLLQRRTMQN
                     AADAGALAAIQVMQEGGIAVTCVNGSGNATTCHPTYLVTSHDVVAKATQFVAANAGGT
                     IGSATPTTLVEYHLMTGAPGCASGCYQTAVDSSTQTVPDWTDGVRVTASIQNPTTFAR
                     AVNISTVPVSAPAAARVYATCTDAADPGPTLPFTRFRPALEEEMLTNGNDRLHPYRFW
                     TSTPDFNGSWKDTLSFRHYSFNNYRASGAADYNTQLLTAFDTRRGLPPGGVGPNYNKT
                     GMSGCPSGTSNCADMGRQKGDGDDLSQDLSNWIYWNWNGTIVLTSTWPSSAGVGSRTG
                     DWSETYKPGDLGNNLQSPILDLIARDGFTTPLSGAPFNLGKALDRTVYVWGPSRTAFV
                     AAGKSDSTVAQKWVDPVPPGHGSGGQDGYFDDITVVQRPNGNYETSGNDPIDRVRFTW
                     AYKFRFYEALQSNNSAVYGLLIEQDEPGPQSGGCTWMPGGGVYSRLSDP"
     gene            2825..>3020
                     /locus_tag="VF276_11230"
     CDS             2825..>3020
                     /locus_tag="VF276_11230"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEX6607475.1"
                     /translation="MNSRRVVKGQTVVIVALCALALLAFVGLAVDGGSTLLQRRTMQN
                     GADAGALAAVQAMTAPGIAVS"
BASE COUNT          561 a          999 c          923 g          537 t
ORIGIN      
        1 acgatctgct gctgcccaac gcccggctat cctggtgccg gcgggcggct cgacggttac
       61 aggggcggat gttccaggcg gaatcggatt tatttgcaat catagttcat ggggcgcgga
      121 aacaggagca atcaccatgc gtaagttgaa cgatctacgg aaacagggcc aggggctggt
      181 cgagtacgcg ctcatcctcg tgctcatcgc cattgtcgtc atcgtcatcc tcgctgtgct
      241 cggcggggct gtgaacgata tcttctcgac catcgccagc gcactctaac cggcccatcg
      301 accggcggag tagatggatc gccgccggca ccgccggcgg cgatccggca aggcgcctcc
      361 agggtatgat acctatgctg agtactaatt ggaaaccgct acatcacgtg gcgaccagca
      421 gcggccgacg cgctcgccag ggcacggcgt tgctcgaact ggcgctcgtc atgccgatcc
      481 tggccgcgct gctgctcggc gtgctcgatt tcgggcgcat gtatgccgaa caactcgccg
      541 tcacctccgc cgcccgcgaa ggcgcccgcg cggccatgca tgataccagc gacaccaatg
      601 tccgcaacgt ggttaccaac gagctaaacg gggccgtggt ccccaacagc agcgggtcga
      661 gttgcggtaa cgccatctgc atcaatcggg acacggtgaa cggacaggtg gtcgtcacgg
      721 tcaactatac tcatagcttc ttgttcggcc tgattcgcaa cggcgacctg gccctggccg
      781 ccagcgccac catgccctac acggcaccat agacggacca tacgaaagta aaaatatgaa
      841 cctttctcgc ttgtctccac gagccaccgc ctatcgccgc gcgccggccc agtcgatggt
      901 cgaattcgcc ctcgtcgcca tggtcctgtt cctgatgctg ttcggcatct tcgaggtctc
      961 gcggctggtc ttcaccatca actcggtgaa caacggcgcc cgcgaaggct ctcactacgc
     1021 cgcgctgcat cccagcgcaa ccgcgaacga tgtcaagcag gccatctcgc cgaccttgtt
     1081 cctgatcaat gtcaacgacg cgtcgaactt ctccctgagc gtggtgacgg ataccacctg
     1141 caccgcgccc tgttcatatt cgccggtgac cgtccggatc agtaacacgt tgcacctggc
     1201 ggtgcccttt cctggagttg ggtccagcgt tgtgattaca tccgcgtcga cagcgctgac
     1261 ggagaggtga gtagtgcgta tgaggagtgc agcaattcaa tggaatgggc gcgttttagg
     1321 aagggacaga cagttattat cgcggcgctg tgcctgctgg ctctcgtggc cttcgtcggc
     1381 ctcgccgtgg acggcgggag caccttgttg cagcggcgca ccatgcaaaa cgccgccgat
     1441 gccggcgcgc tggccgccat ccaggttatg caggaaggcg gcatcgccgt gacctgcgtg
     1501 aacggttcgg gcaacgccac cacctgccac ccgacgtacc tcgtcaccag ccacgatgtc
     1561 gtcgcgaagg ccacgcagtt cgtggccgcg aacgccggcg gcaccatcgg cagcgccacc
     1621 ccgaccacgc tggtcgagta tcacctcatg accggcgcgc ccggctgcgc gagcggttgc
     1681 tatcaaaccg ccgtggacag cagcacgcaa accgtgcccg attggaccga cggcgtgcgc
     1741 gtcacggcca gtatccagaa tccgaccacg tttgcccgcg ccgtcaacat cagcaccgtg
     1801 ccggtgagcg ccccggcggc ggcccgcgtc tacgccacct gcaccgacgc cgccgacccc
     1861 ggcccgaccc tgcctttcac ccggttccgg cctgccctcg aagaagagat gctgaccaac
     1921 ggcaacgacc ggctgcatcc ctatcggttc tggacatcaa cgcccgattt caacggcagt
     1981 tggaaggaca ctctttcttt ccggcattat agcttcaata attaccgcgc cagcggggcc
     2041 gcggactaca atacccaact gctgacggcg tttgatacgc ggcgcgggct acctccgggc
     2101 ggcgtcggcc ccaactataa caagaccggg atgagcggct gtccgtcggg caccagtaac
     2161 tgtgccgaca tgggccggca gaagggcgac ggcgacgatc tgtcgcagga tctctccaac
     2221 tggatttact ggaactggaa cggcaccatc gtgctcacca gcacctggcc gtctagcgcc
     2281 ggcgtgggat cgcgcaccgg cgactggtcg gagacctaca agcccggcga cctgggcaac
     2341 aaccttcaat ccccgatcct tgacctgatc gcgcgggacg gcttcacgac cccgctcagc
     2401 ggcgcgccgt ttaacctcgg caaggcattg gaccgcacgg tctatgtctg gggtccctcg
     2461 cgcaccgcct ttgttgccgc cgggaagagt gactcgaccg tcgcccagaa atgggtcgac
     2521 ccggttccgc cggggcacgg cagcggcggc caggatggtt actttgacga catcaccgtc
     2581 gtccagcggc caaacggcaa ctacgagacc tcgggcaacg atcccatcga ccgggtgcgt
     2641 ttcacctggg cctacaagtt tcgcttctat gaggcgctgc aaagcaacaa tagtgccgtg
     2701 tacggcttac tgatcgagca ggacgaacca gggccgcagt ccgggggctg cacctggatg
     2761 ccgggtgggg gcgtgtactc gcggctcagc gatccgtgag ccggcgcaac aaggattgag
     2821 catgatgaac agcaggcgtg tcgtcaaagg acaaacagtg gtaatcgtgg cgctttgcgc
     2881 cctggcgctg ctggcgtttg tgggcctggc cgtggacggc gggagcacct tactccagcg
     2941 acgcaccatg cagaatgggg ctgatgcggg ggcgctggcc gccgtccagg ccatgacggc
     3001 gccggggatc gccgtgtcct
//
LOCUS       DASUYC010000686         4923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1360722, whole genome shotgun sequence.
ACCESSION   DASUYC010000686 DASUYC010000000
VERSION     DASUYC010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4923
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1360722"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..838)
                     /locus_tag="VF276_11235"
     CDS             complement(32..838)
                     /locus_tag="VF276_11235"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6607476.1"
                     /translation="MPDASAAPASPARDLSTRQLVQEGAEPFFYAAGPTGCLLLHGFT
                     SSPFTMREMGAHLAASGVTVCAPLLAGHGTSPEDLRTKTWQDWVASADEGLAQLRAYG
                     CRRIYLAGLSLGGALTLYLAARDPNAYAGMVVMSAPVWIPAMLRLPLTAVSDAVPYFN
                     KRFSDIAEPGARQRQLTYSRIPLAAAATLIDLLSEMRPALGRITIPALIIYARHDHLV
                     HPVNSMHIYSHIASRDKRLVVLHRGFHIVTVDRDKDRVFQLAAEFMHRED"
     gene            complement(825..2312)
                     /gene="guaB"
                     /locus_tag="VF276_11240"
     CDS             complement(825..2312)
                     /gene="guaB"
                     /locus_tag="VF276_11240"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX6607477.1"
                     /translation="MLSDAEKFGLEGLTFDDVLLVPAYSEVLPTPQYISTATRLTRDI
                     HLNIPILSAAMDTVTEARMAIAMAREGGLGVLHRNLAIADQVAEADKVKRSQAGMIVE
                     PICLEPERSVRDALDLMARYHISGVPIVEGDRLVGILTNRDIRFAEGADLDQPVRQWM
                     TEAPLWTVPPGTTLDQAREILRRRKVEKLPVVDENGRLVGLITVKDIQKKQQYPLATY
                     DRHGRLLCGAAVGVGDDGFERAAALIEVGVDVIFVDTAHGHTASVIEAVRRIKRRYDV
                     PVIAGNIATGAAAQALIDAGADGLKVGMGPGSICTTRVVSGAGMPQITAVWEVARVAR
                     PAGIPIIADGGIQYSGDVAKAIATGADTVMIGSLLAGVDESPGDVILYQGERFKEYRG
                     MGSLGAMKQRASSRDRYGQQDVDRLDKLVPEGIEGRVQYKGPLTTLMYQLVGGLRSGM
                     GYVGAPDIARFQEDTRFVRVTGSGLRESHPHDVVLTKEAPNYAGR"
     gene            complement(2337..3389)
                     /locus_tag="VF276_11245"
     CDS             complement(2337..3389)
                     /locus_tag="VF276_11245"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEX6607478.1"
                     /translation="MNLLALLALITGLALAPALLYVLLIWWLDRYEKEPISLAVLAFV
                     WGATPAILLALVFEILLGIPLGGAGPIGEFSEATVVAPIVEEATKGIILFVLFLAYRH
                     EFDDVLDGILYGALVGLGFSVVENIVYGFSSAYPDGYNPGQTPSLDAVIGIWFLRSGV
                     FGLNHAFFTSITGATLGYLRANPKARLRPVMPVFGLGGAMFFHSLHNSLVTVSGLAGN
                     ELACAGCLISILADWSGVAVIVVVAILAGAKERRWIEQQLWEEVTVGRLHPAEYYMLI
                     SAPRRWRARWAALSQHGWRAFRQLGLLQQLATELAFRKQQVLVERDSAWRQYDIQVLR
                     ARIDDLHTRMAGASPA"
     gene            3818..4459
                     /locus_tag="VF276_11250"
     CDS             3818..4459
                     /locus_tag="VF276_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6607479.1"
                     /translation="MPIRVLIADDHEVVRQGLHFFLHLDPDLELVGEAEDGAEAVRLA
                     RELHPDVVLMDLLMPEIDGITATATIRRELPETEVLALTSVLEDTAVVGAMRAGAIGY
                     LLKDTRAEALCRAIKAAAAGQVQLAPEAASRLMREVRAPETPESLTERETEVLRLLAR
                     GYSNKEIAGRLNIVERTVKTHVHGILQKLSLPSRTQAALYAVRTGLVNPNEQN"
     gene            4474..>4923
                     /locus_tag="VF276_11255"
     CDS             4474..>4923
                     /locus_tag="VF276_11255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX6607480.1"
                     /translation="MSDPPAPLPERRAQYRRADDDRFQLAVEGANDGIWDWDIENNTV
                     YLSPRWKSLLGYADHEISNNLVEWLSRIHAEDRARVIAGVTDYLEGTTTNYEIEYRLR
                     HKDGSWRWVISRGSARRYDDGRPYRLAGSNTDITARKQAEAQLRETEA"
BASE COUNT          869 a         1647 c         1645 g          762 t
ORIGIN      
        1 tcccccatat cgaacaggac ggctttgagc attaatcttc ccggtgcatg aattcggcgg
       61 caagctggaa cacgcggtcc ttatcccggt cgacggtgac gatatggaaa ccgcggtgca
      121 gcacgacgag gcgcttgtcg cgcgaggcga tatggctgta gatgtgcatc gagttcaccg
      181 gatgcacgag gtggtcgtgg cgcgcgtaga taatcagcgc ggggatggtg atgcgcccca
      241 gcgcgggccg catctcgctc agcaggtcga tcagcgtggc cgcggcggcc agcgggatgc
      301 gcgagtaggt cagttgccgc tgccgcgcgc cgggttcggc gatgtcgctg aagcgcttgt
      361 tgaagtaagg cacggcgtcg ctgacggcgg tgagcggcag gcgcaacatg gcggggatcc
      421 agaccggcgc gctcatgact accatcccgg cgtaggcatt gggatcgcgg gcggccaggt
      481 agagcgtgag cgcgccgccg agcgacagcc cggccaggta gatgcggcgg cagccgtagg
      541 cccgcaactg ggccaggccc tcgtcggcgg aggccaccca gtcctgccag gttttggtgc
      601 gcaggtcttc gggcgaggtg ccgtgcccgg ccagcagcgg ggcgcagacg gtcacgccgc
      661 tcgccgccag gtgcgcgccc atctcgcgca tggtgaacgg cgacgaggtg aagccgtgca
      721 gcagcaggca cccggtcggc ccggcggcgt agaagaacgg ctcggcaccc tcctgcacca
      781 gttggcgcgt ggacagatcg cgggccggcg atgccggggc cgcgctagcg tccggcataa
      841 ttcggcgcct ctttcgtcag caccacgtcg tgcggatggc tctcgcgcag gcccgacccg
      901 gtcacgcgca cgaagcgggt gtcctcctgg aagcgcgcaa tgtcgggcgc gccgacatag
      961 cccatgcccg agcgcagccc gcccaccaac tggtacatca atgtggtcag cgggcctttg
     1021 tactgcaccc ggccctcgat gccttcgggc accagtttgt ccaggcgatc cacgtcttgc
     1081 tggccgtagc ggtcgcggct ggaggcgcgc tgcttcatgg cgcccagcga ccccatgccg
     1141 cggtactcct tgaagcgctc gccctggtag aggatcacgt cgccggggct ttcgtccacg
     1201 cccgccagca gcgagccgat catcacggtg tcggcgcccg tggcaatcgc tttggcgaca
     1261 tcgccactat attggatgcc gccgtcggcg atgatcggga tgcccgccgg gcgggccacg
     1321 cgagccacct cccagaccgc ggtgatctgc ggcatccccg cacccgacac cacgcgcgtc
     1381 gtgcagatcg accccggccc catacctacc ttcaagccgt cggcgcccgc gtcgatcagc
     1441 gcctgggccg ccgcgccggt ggcgatattg cccgcgatca ccggcacgtc gtagcggcgc
     1501 ttgatgcggc gcaccgcctc gatcacgctc gccgtgtggc cgtgcgcggt gtccacgaag
     1561 atcacgtcca cgccgacctc aatcagggcg gcggcgcgct cgaagccgtc gtcgcccacg
     1621 ccgaccgccg ccccgcagag caggcgcccg tgccggtcgt aggtcgccag cggatattgc
     1681 tgctttttct ggatgtcctt gacggtgatc aggccaacca ggcggccgtt ctcgtccacc
     1741 acgggcagct tttcgacctt gcggcggcgc agaatctcgc gcgcctgatc cagtgtggtg
     1801 ccgggcggca ccgtccagag cggcgcctcg gtcatccact ggcgcaccgg ctgatccagg
     1861 tcggcgccct cggcgaaccg aatatcgcgg ttggtcagaa tgccgaccag ccggtcgccc
     1921 tcgacgatgg gcacgccgga gatgtggtag cgggccatca ggtcgagcgc gtcgcgcacc
     1981 gagcgctccg gctccagaca gatcggctcg acgatcatgc cggcctgcga ccgcttcacc
     2041 ttgtcggcct cggccacctg gtcagcgatg gcgaggttgc ggtgcagcac gcccagcccg
     2101 ccctcgcggg ccatagcgat cgccatgcgc gcctcggtca cggtatccat cgccgccgac
     2161 aggatcggga tgttcaggtg gatgtcgcgc gtgaggcggg tggccgtgga gatatactgg
     2221 ggcgtgggca agacttctga ataggcgggc acgagcagaa catcgtcgaa cgtcagccct
     2281 tccaaaccaa acttctccgc atcagacagc atcagacatc tcccttccgg gttctgctac
     2341 gccgggctgg cgccggccat gcgcgtatgt agatcgtcga tccgggcgcg caacacctgg
     2401 atatcgtact ggcgccaggc cgagtcacgc tcgaccagca cctgttgctt gcggaaagcc
     2461 agctcggtcg ccagttgctg caagaggccc aactggcgga acgcccgcca tccgtgctgc
     2521 gatagggccg cccagcgcgc gcgccagcgg cgcggcgccg agatcagcat atagtattcg
     2581 gcgggatgca gccgccccac cgtcacctct tcccagagtt gctgctcgat ccagcggcgc
     2641 tccttggccc cggccaggat cgccaccacg acaatgacgg ccacgccgct ccagtcggcc
     2701 aggatgctga tcaggcaccc ggcgcaggcc agttcgttgc cggccagccc gctcaccgtg
     2761 acgaggctgt tgtgcaggct gtggaagaac atcgctccac ccaacccgaa cacgggcatg
     2821 accggccgca gccgcgcctt ggggttggcg cgcagatagc cgagtgtggc cccggtgatg
     2881 ctggtgaaaa aggcgtggtt caggccgaac acccccgacc gcaagaacca gatgccgatc
     2941 acggcgtcga gcgaaggcgt ctggccgggg ttgtagccgt cgggataggc cgagctaaag
     3001 ccgtagacaa tgttctccac gaccgagaag ccgaggccga cgagcgcgcc gtagaggatg
     3061 ccgtcgagca catcgtcgaa ctcgtgccgg taggccagga agaggacgaa gaggatgatg
     3121 cctttggtcg cttcctcgac aatgggcgcg acgaccgtcg cttcgctgaa ctcgccgata
     3181 ggcccggcgc caccgagggg aataccaagc aggatctcga agacgagcgc gaggagaatc
     3241 gccggggtgg cgccccagac gaaggccagg accgccaggc tgatcggctc tttctcatac
     3301 cgatccagcc accagatcaa caagacatag aggagcgccg gagccagggc cagcccggtg
     3361 atcagggcca gcagcgccag caagttcata gacgatgcgg ttccttcccc agtattgagc
     3421 gatcggcgca cgggccgccg gatgacggcg ggctcaccct atcccatcat agggcagcgg
     3481 tacaagctta tcgggtattg tacccgattg ccggggatgg cggcaagaca cgcgcgacga
     3541 gtcgagcggg ccaatgtagg accatcgggc cggggccgct gggagcggaa tcctatggcg
     3601 cggggcgcgc gctccccata atcagacggc ttgaggccgg gccgcaacgg tggtcatgta
     3661 gttgtaggcc accggcaatt gtgctacaat ggggataagg acaattacgc cctgtagttt
     3721 tgctgaacca tgttggcctg ccctcgtttc gcaccaccga aagcagccga tcgcgcgcct
     3781 tcgctagcga aggcacgcta cccagaggct actgctcatg ccgattcgtg tcctgatcgc
     3841 cgatgaccac gaggtggtcc gccagggcct gcatttcttt ctccatctcg atcccgacct
     3901 ggaactggtg ggggaagccg aagacggggc cgaagccgtg cgcctggccc gcgaactgca
     3961 cccggatgtc gtgctgatgg atttgctgat gccggaaatc gacggcatca cggcgaccgc
     4021 gaccatccgc cgcgagctgc ccgagaccga agtcctggcg ctgaccagcg tgctggagga
     4081 tacggcggtc gtcggggcga tgcgcgcggg cgccatcggc tatttgctca aggatacgcg
     4141 cgccgaagcg ctctgccggg ccatcaaggc cgcggcggcg ggccaggtcc aactggcccc
     4201 ggaagccgcc tcccggctca tgcgcgaggt gcgcgccccg gaaacccccg aaagcctgac
     4261 cgagcgcgag accgaagtgt tgcgcttgct ggcgcgcggc tactccaaca aagagatcgc
     4321 ggggcggctg aatattgtgg agcgcaccgt gaaaacccat gtccacggca tcctgcagaa
     4381 actgagcctg cccagtcgca cccaggcggc gctctacgcc gtacgcacgg ggctggtcaa
     4441 ccccaacgag cagaactagc cggggattca gctatgagcg acccgcccgc cccgctaccg
     4501 gagcgccgcg cccaatatcg acgcgccgac gacgaccgct tccagctcgc ggtcgagggc
     4561 gccaacgacg gcatctggga ttgggatatc gagaacaata ccgtttacct ctcgccgcgc
     4621 tggaaaagcc tgctcggcta tgccgatcac gagatctcca ataacctggt cgaatggctg
     4681 tcgcgtatcc atgcggagga tcgcgcccgc gtcatcgccg gggtgacgga ctatctggaa
     4741 ggcaccacga ccaattacga gatcgaatac cggctgcggc acaaggatgg gtcctggcgc
     4801 tgggtgatct cgcgcgggtc ggcccggcgc tacgacgatg gccggcccta tcgcctggcc
     4861 ggctcgaaca ccgacatcac cgcacgcaag caggctgagg cccaactgcg ggaaaccgag
     4921 gcc
//
LOCUS       DASUYC010000687         2024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1053321, whole genome shotgun sequence.
ACCESSION   DASUYC010000687 DASUYC010000000
VERSION     DASUYC010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053321"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VF276_11260"
     CDS             <1..429
                     /locus_tag="VF276_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607481.1"
                     /translation="PMHYNSIDHFLTVHEPDIAALIEDAERVTGGHYAAMAPDQLHRT
                     AQSDARELIAGLRAAHVDRDVVQETARQTETQGIALDDLTRMSIEIERRFIPYIQAEL
                     AEQPEFAADLVRRCRHITAGYRSNVTAVKLDRALGRLKHS"
     gene            536..1042
                     /locus_tag="VF276_11265"
     CDS             536..1042
                     /locus_tag="VF276_11265"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX6607482.1"
                     /translation="MFTPETAADSTTIRLRVRYAETDAMGIVHHSRYLPWLEMARTEF
                     MRERGYTYRELEQAGVQLAVIEVQARYRAPAVYDDPIAVTARLAEFGRVKLRFEYRVY
                     NEASGRLLMEAETLHAFVGRNGQPVRMASQYPEIWQRLQAIVSEVRRGEGGTDDGTAP
                     PGGHPARD"
     gene            1002..>2024
                     /locus_tag="VF276_11270"
     CDS             1002..>2024
                     /locus_tag="VF276_11270"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6607483.1"
                     /translation="MALRPQAATPPATNGTGNGAHPAELVSYDPATGAELGRVPIRSA
                     AEVRAAVARARAVQPTWAALPLAARLAYLRRIRAVLAEHADELSELTAREMGKHPTEA
                     LLGDTLFSLTHLDYVINMAPRLLRPRRVRHGIVFATRYAQVVLDPRGVIGIISPYNYP
                     VLMTANTLFMALAAGNTVVAKPSEHTPLTVLKLAEICHAAGLPADVFQVLTGDHTTGA
                     ALVDSDIDGLVFVGGTAAGRKIAAAAGARLLPVTMELGGNNAMIVLEDADLQMAARAA
                     IWGGFVTSGQVCGRAGRVLVAAPMAERFLAAVTREAERVRHAAQVAEGVDMGPLTTAV
                     SLAHVRR"
BASE COUNT          333 a          732 c          662 g          297 t
ORIGIN      
        1 ccgatgcact acaactccat cgatcatttc ctgactgtcc atgagccgga catcgcggcg
       61 ctgatcgagg acgccgagcg cgtcaccggc ggccattatg ccgccatggc gcccgaccaa
      121 ctccaccgca ccgcgcaatc cgatgcgcgc gagttgatcg cgggcttgcg ggccgcccac
      181 gtggaccgcg atgtcgtcca ggaaaccgct cgccagacgg agacgcaggg catcgccctc
      241 gacgacctga cgcgcatgag tatcgagatc gagcgccggt tcattcccta tatccaggcc
      301 gaactggccg agcaacccga gtttgccgcc gacctggtgc ggcgatgccg ccacatcacc
      361 gccggctacc gcagcaatgt caccgccgtc aagttggatc gcgccctggg ccgcttaaag
      421 cactcctgaa ccggatcgta ctcgtgcagc ctcgcgcacc cgccacactc acccggcggc
      481 atagcggttg cttgcacaac cgtgactttt accctgaaag gattacccgc accctatgtt
      541 cacgcccgag accgcggcgg atagcacgac cattcgcctg cgcgtgcgct acgccgaaac
      601 cgacgccatg ggcatcgtcc accacagccg ctacctgccc tggctggaga tggcacgcac
      661 cgagttcatg cgcgagcgcg gctacactta ccgcgaactg gaacaggcgg gtgtgcagct
      721 tgccgtgatc gaggtccagg cccgctatcg cgcgcccgcc gtctatgacg atccgattgc
      781 cgtcacggcg cggctggccg agttcgggcg ggtcaagttg cgctttgagt accgggtgta
      841 taatgaagca tccggccgcc tgctgatgga ggccgagacc ctgcatgcat ttgtcggccg
      901 caacggccaa cccgtccgca tggccagcca gtatccggaa atctggcagc gactccaggc
      961 aatcgtgagc gaggtgcggc gcggcgaagg aggaacagac gatggcactg cgcccccagg
     1021 cggccacccc gcccgcgact aacggcaccg ggaacggtgc gcacccggcg gaactggttt
     1081 cctacgatcc cgcgaccggc gccgagttgg ggcgcgtccc gatccgcagc gccgcagaag
     1141 tgcgtgcggc ggtggcgcgc gcccgcgcag tacagccaac ctgggccgcg ctgcccctgg
     1201 ccgcccggct cgcctacctg cgccgtatcc gcgccgtcct ggctgagcac gccgatgaac
     1261 tgagcgaact cacggcgcgc gagatgggca agcacccgac cgaggcgctg ctgggcgaca
     1321 ccctgttcag cctgacccac ctggactacg tgatcaacat ggcgccccgg ctgctgcggc
     1381 cccgccgcgt gcgccacggc atcgtcttcg ccacgcgcta cgcccaggtc gtgctcgacc
     1441 cgcgcggcgt gatcggcatc atctcgccct acaactaccc ggtgctgatg acggccaaca
     1501 cgctcttcat ggccctggcc gcgggcaaca cggtcgtcgc caagccctcg gagcacaccc
     1561 cgctgacggt gctgaagctg gccgagatct gccacgccgc cgggctgccc gccgacgtgt
     1621 tccaggtgct gacgggcgac cataccaccg gcgcggcgct ggtggacagc gacatcgacg
     1681 ggctggtctt tgtcggtggc accgctgccg ggcgcaagat tgccgccgcc gcgggcgcgc
     1741 gcctgctgcc ggtgacgatg gaactgggcg gcaacaacgc tatgatcgtg ctcgaagacg
     1801 ccgacctgca aatggcggcg cgggcggcca tctggggcgg ctttgtcacc agcggccagg
     1861 tctgcgggcg ggccgggcgc gtgctggtgg ccgcgccgat ggccgaacgt ttcctggccg
     1921 ccgtcacccg cgaagccgag cgggtgcggc acgccgcgca ggtggccgag ggcgtggaca
     1981 tgggaccgct gaccaccgca gtgagcctgg cccacgtgcg ccgg
//
LOCUS       DASUYC010000688         2085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1320456, whole genome shotgun sequence.
ACCESSION   DASUYC010000688 DASUYC010000000
VERSION     DASUYC010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1320456"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VF276_11275"
     CDS             <1..331
                     /locus_tag="VF276_11275"
                     /inference="COORDINATES: protein motif:HMM:NF014085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HEX6607484.1"
                     /translation="LLGDLPLLEAADVRAMAEIAADPIRGAPVVVLAPDRHGHGTNAL
                     LQRPPGIVPFEFGHDSFPRHFRAAAANRAEIMIYRSPGTAFDLDTPGDLADLPAGSPL
                     HFAARPT"
     gene            387..1148
                     /gene="cofE"
                     /locus_tag="VF276_11280"
     CDS             387..1148
                     /gene="cofE"
                     /locus_tag="VF276_11280"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEX6607485.1"
                     /translation="MPASSEIRILALGAFPEVTPGMDLPPLILAAAADAGVTLAPGDI
                     LVVTQKIVSKAEGALVDLRTIEPSDLAARWAATWGKDPRQIEVVLRESKRIVRMANGL
                     IISETQHGFVCANAGVDASNVAGHDVVSLLPRDPDASAARLRETLSAELGFDLPVIIS
                     DSFGRPWRYGIINVAVGLAGLGAVADYRGQTDSHGYLLNASILAVADELAAAAELIMG
                     KLDGRPVAVIRGYAYEAANSTAQDLLMDPARDLFR"
     gene            1408..>2085
                     /locus_tag="VF276_11285"
     CDS             1408..>2085
                     /locus_tag="VF276_11285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX6607486.1"
                     /translation="MGTLGYAAMFEQFTPTDLLRYSQQAEQAGFTAVMASDHFHPWTP
                     GQGQSGFVWAWMGALGATTQLRFGTGVTPPGYRYHPAVLAQAAATLEAMFPGRFYLGL
                     GAGEALNEHIVGGYWPEPVARLEILGEAIEIIRKLFSGKVVKYRGQHFTLESAKLYTL
                     SDPPPPIYVATSGPVNAERTGATCDGIITVGAADEKIKMLLGRFEKGAEGAGKDPSKM
                     ARILQLHV"
BASE COUNT          320 a          695 c          721 g          349 t
ORIGIN      
        1 gctgctcggc gatctgccct tgctggaagc cgccgatgtc cgcgccatgg ccgagatcgc
       61 cgccgacccg atccgcggcg cgcccgtcgt ggtgctggcg ccggaccggc acggccacgg
      121 caccaacgcc ctgctgcagc gcccgccggg catcgtgcct ttcgagttcg gacacgacag
      181 cttcccccgc cacttccgcg ccgccgccgc caaccgcgcg gagatcatga tctaccgctc
      241 gccgggcacc gccttcgacc tcgatacgcc gggcgacctg gccgacctgc cggcgggatc
      301 gccgctgcac ttcgccgccc ggcccacctg atagcaaagg gcgccgtcgc ttgttggttc
      361 tgttatgtca acttgagagg actcttatgc ccgcttcctc ggaaatccgg attctcgcgc
      421 tgggcgcctt tcccgaagtc acccccggca tggacctgcc gccgctgatc ctggccgccg
      481 ccgcggacgc gggcgtaacc ctcgcgccgg gcgatattct ggtggttacg cagaagatcg
      541 tgagcaaggc cgagggcgcg ctggtcgatc tgcggaccat cgagccgtcg gacctggcgg
      601 cgcgctgggc cgcgacctgg ggcaaggacc cgcgccagat cgaggtggtg ctgcgcgaga
      661 gcaagcgcat cgtgcgcatg gcgaacggtc tcatcatctc cgagacccag cacggcttcg
      721 tctgcgccaa tgccggggtc gatgcatcga acgtggccgg gcacgacgtg gtctcgctcc
      781 tgccccgcga ccccgacgcc tcggcggcgc ggctgcgcga gactctgagt gcggaactcg
      841 gcttcgacct gccggtgatc atctcggatt cgtttggccg gccctggcgc tacgggatca
      901 tcaacgtggc ggtggggctg gcgggcctgg gcgcggtggc cgattaccgg ggccagaccg
      961 acagccacgg ctatctgctg aacgccagca tcctggcggt cgccgatgag ctggcggcgg
     1021 cggcggaact gatcatgggg aagctggatg ggcggcctgt ggccgtcatc cgggggtatg
     1081 cgtatgaggc ggcgaacagc acggcccagg atctgctgat ggatcccgcg cgcgatctgt
     1141 ttcgctagcc ctgttgggga tcagcgcccg atgataggcg cgctgacgat gaacgagtac
     1201 ggcgcgatga atcgcgcggc tacgaatcgc gtggagacgc cgcgagaaat cgcgtggcgt
     1261 tgcgcttgat aaagtgaggg tggtccggac ctcccgcgag caggtcggcg ttggctccgc
     1321 ttgctctgtc tatatcaacg gagcaggcgg ggcggggttg catccttttc gcacgaatcc
     1381 gggacgagtt ttaaggagga ttttgtcatg ggtacgctgg gttacgccgc catgttcgag
     1441 cagttcacgc ccaccgatct gctccgctat tcgcaacagg ccgaacaagc cgggtttacg
     1501 gcggtcatgg cctccgacca cttccacccc tggacgccgg gccagggcca gagcggcttt
     1561 gtctgggcct ggatgggtgc gctgggcgcg acgacgcagt tgcgcttcgg cacaggggtg
     1621 acgccgccgg gctatcgcta ccacccggcg gtgctggccc aggccgccgc cacgctggaa
     1681 gccatgttcc ccggtcgctt ctacctgggg ctgggcgcgg gcgaggcgct gaacgagcac
     1741 atcgtcggcg gctactggcc cgagccggtg gcccggctgg agatcctcgg cgaggccatc
     1801 gagattatcc gcaagctgtt cagcggcaag gtggtcaagt atcgcggcca gcacttcacc
     1861 ctggaaagcg ccaagctata taccctgtcc gacccgccgc cgcccatcta cgtggcgacc
     1921 tcgggcccgg tgaacgccga aaggacggga gccacctgtg acggcatcat caccgttggc
     1981 gccgccgacg agaagatcaa gatgctgctg gggcgcttcg agaagggggc cgagggcgcg
     2041 ggcaaggacc cgtccaagat ggcgcgcatc ctccagttgc acgtc
//
LOCUS       DASUYC010000689         8687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_717088,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000689 DASUYC010000000
VERSION     DASUYC010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 8687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8687
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717088"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..1208
                     /locus_tag="VF276_11290"
     CDS             27..1208
                     /locus_tag="VF276_11290"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEX6607487.1"
                     /translation="MNPTTNENGLLFELSGTQTPRIVIEHLNGDVTIEGWERNAVQVG
                     ARDKRDAEGLEELLDVEQHGNDLKIKLDLGGAARRRARRHREDFENFDDFAEGMAEMR
                     EVFRHLGKSFNADLVVQVPHTCDITVRTFNGDVSLHRVQGKLYVQNTSGDISLHEIRG
                     NVLVKSASGDVEIKELRGRLGVRTASGDLSIQDSELAALSAGTASGDVQFLGALLPAE
                     DYEIQTVSGDVEFVLPENRRASVEMQTVSGDISCSRGLTHEIEKRNKRHRIVHINGGG
                     EVIVRVRTTSGDLTVTPGPEYHGGYSADMPAAEVSVAPEADAGDETRAFTDDEATAAR
                     VAMQGDGGGVEPGQTQRLDAHAEDRSGPRKSAEMTILDAIENGEMSVDEGLRRLNELA
                     Q"
     gene            1305..2354
                     /locus_tag="VF276_11295"
     CDS             1305..2354
                     /locus_tag="VF276_11295"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6607488.1"
                     /translation="MPVTTVRIEPLDPHAITEDQIAAVHVLMSRSEAERFPNDPPRPL
                     EQTAALFRNIPPFFDLRIWAAGTEDRSTLLGYGILVMPRTEENQHAAQIEMEVLPEAR
                     RHGLAKRLLIPIVDAAAQAGRRLLIANTNSNQPGGAALMERLGAGAALVGHINQLVVA
                     GVDRAMLRAWQEHAETHAPNFTLGLWEGAYPDSHLDEIAALFQAMNLAPRGDLDVEDF
                     RWTPEQMRQIQDMKIGRGGIPWTMYLRDSTTGELAGFTEINWNPSQPGVVWQGGTAVW
                     PKYRNQGLGRRLKAAMLEKVLRDRPQVEYIRTENADSNAAMLKINQELGFRPYYSETT
                     WQVPVDQVRAYLGTT"
     gene            2619..3878
                     /locus_tag="VF276_11300"
     CDS             2619..3878
                     /locus_tag="VF276_11300"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6607489.1"
                     /translation="MIAVLRQRNFALLWFGGLISMAGDWVLRIGLPLYIYQVTGSTLA
                     TGIMFMAAMLPSLLLGSVAGVFVDRWDRKQTMVIANVLLAGSLLPLLLVRSAEWVWIA
                     YIAAFAQSCIAQFFDPAESAMLPLLVGEEHLPTANALNALNNNLARLIGPAIGGVVLA
                     VGGLTGVALVDAASFLIAAGMIALITANGRAVRAETPAAAEGAGGRWLAVWREWVEGL
                     RIVVQTRTVAIIFLLLGITSLGEGVISVLFAPFVSKVLGGGGTEFGAIASAQAVGGLI
                     GGALIGEVARRVRPGRLIGVCGILFGLIDLAIFNYPVFGAAVILMVLVGLPGVGFSTG
                     VTTLLQTSTANQYLGRVFGALGTTASLLALIGAGLASVLGDRIGIVPVLNLQGAGYIL
                     AGALALVLLAHVALPRAQAPAEESVPA"
     gene            4084..5517
                     /locus_tag="VF276_11305"
     CDS             4084..5517
                     /locus_tag="VF276_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015234178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6607490.1"
                     /translation="MSYEPPPNGFRTFLIVWCTQSVSVFGSALTFFAVTIWLTQVLYP
                     DPAQKPELAAALAAVSLAFSLPTLFIAPIAGAWADRHDRKHIMMAGDATNGTLSLVLM
                     ALMATGSLQLWSLLLITVLMALAGSFHGAAFDTSYSMLVPEKQLPRANGMMQTMWSLS
                     GILSPAIAAGIISLPALARQGLLPFDLNGALAELKDGTPLAITIDAITFFAAGATLLF
                     LRIPSPVRTDLQKEAGGPRKSLWSDVREGALYIWHRKPLLWLLGTFTVANFAGGPVEV
                     FMPLLLKFNLAADWAARGFTFETALALMGSVASIGGVLGGLLVSAWGGLKKRRIYGVL
                     VPMIIGGVAQIAFGFSPLLFLSAAMAGVVTGMVPILNAHSQSIWQTQTPRELQGRVFS
                     VRRVIAQCTWPLSTALAGLAGGFFNPGLVIAALGAFLLIFCVAQLFNPYLRRVEDKDW
                     LDSLAARHGPAAPPEPATAILETADKT"
     gene            5514..6728
                     /locus_tag="VF276_11310"
     CDS             5514..6728
                     /locus_tag="VF276_11310"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6607491.1"
                     /translation="MTVVGALRQRSFAWLWTGQAISRLGDSIHRVALSWWVLEQTGSA
                     AVMGTVLIFSFTPMLLFLLIGGVAGDRLPRVRLMLGSDLLRGGVVALVAALAATHTLQ
                     IWHLYVASMVFGLLDAFFQPAYTALVPEITPAPLLPSANSLTSLSGQLSNIAGPAIGA
                     GIVAAGGTALAFAVDSLSFFLSAACLIPLIGLPQAPAPAGHGARHMLRDLREGVRAVL
                     AAPWLWVTIALAALFNITQGGPLAVALPFLVKDSLHADVGVLGLLYSMTSVGAVLSAI
                     WLGRARRIRRRGLLAYLGLLAGGLTTLMLGLPVPLPAVAVVILLNGAAVTVFGLIWTN
                     TLQELVPRDLLGRVASIDMLGSFALLPIGYGIAGWATDLLGPALVFITGGALTCALVL
                     IGLAHPGIRNLD"
     gene            complement(6938..8008)
                     /gene="secF"
                     /locus_tag="VF276_11315"
     CDS             complement(6938..8008)
                     /gene="secF"
                     /locus_tag="VF276_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX6607492.1"
                     /translation="MFDLVGKRYLFFLVSLIIIIPGVISLFLFGLRLGIDFTGGTLWE
                     VVPRSAEVNTNEVAQLLKDNGQTDVAVQEANLIVGNVTTRTLIMRMPNISPDEKDALT
                     KVLVDRGIISGTEQTVSVSSTAPVTATATTTGTTAPAVETTTVTRIAPGDEIQFNTVG
                     PTIGSEVASRAILSVLAASLGILAYLWYAFRRVPNAIRYGVCAVIALLHDVLVVVGIF
                     SILGELFGIEVDALFITAMLTVIGFSVHDTIVVFDRIRENLLKRRFDSFEKVVNYSLV
                     QTLTRSINTSLTVIFTLSALFLFGGVSIRDFVLALLIGIVSGTYSSIFNASMMLVVWE
                     NKEWRNWFGRGKTADTATARTA"
     gene            complement(8115..>8687)
                     /locus_tag="VF276_11320"
     CDS             complement(8115..>8687)
                     /locus_tag="VF276_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX6607493.1"
                     /translation="VDQSLIAGIIGLSLVMLFMLLYYHLPGLLADIALLIFACITFAI
                     FRAVPVTLTLAGIAGFILSIGMAVDANVLIFARLKEELRSGKTVAAAVEAGFDHAWPS
                     IRDSNMTTVIITLILYWFGNFFGASVIKGFALTLLISVLVSLFTAITVTRTLLRLVVS
                     TRMARNPWWFGVESTARPTLPPEGGTQPVR"
BASE COUNT         1423 a         2847 c         3005 g         1412 t
ORIGIN      
        1 ccgggggcgt cacaaaggag tctggtatga atccgacgac gaacgaaaac gggctgttat
       61 ttgagctgag cggcacgcag acgccgcgca tcgtgatcga gcatctgaac ggcgatgtca
      121 ccatcgaagg ctgggaacgt aacgccgtcc aggtcggggc gcgcgacaag cgcgacgccg
      181 agggcctgga ggagttgctg gatgtcgagc agcacggcaa cgacctgaag atcaagctcg
      241 acctgggcgg cgcggcccgg cggcgcgccc gccggcaccg ggaggatttc gagaatttcg
      301 acgattttgc cgagggcatg gccgagatgc gtgaagtgtt ccgccatctg ggcaagagct
      361 tcaacgccga cctggtggtg caggtgccgc atacctgcga catcaccgtg cgcaccttca
      421 acggcgatgt gagcctgcac cgggtccagg gcaagctcta cgtgcagaac accagcggcg
      481 acatcagcct gcacgagatc cggggtaacg tgctggtcaa gagcgcgtcg ggcgacgtgg
      541 agatcaagga gttgcgcggc cgcctgggcg tgcgcaccgc cagcggcgac ctgtcgatcc
      601 aggatagcga actggcggcg ttgagtgccg gcacggccag cggcgacgtg cagttcctgg
      661 gtgcgctcct gcccgccgag gactatgaga tccagacggt cagcggcgat gtggagttcg
      721 tgctgccgga gaaccgccgg gccagcgtcg agatgcaaac agtcagcggc gacatctcgt
      781 gcagccgcgg cctaactcat gagatcgaga agcgcaacaa gcgccatcgc atcgtgcata
      841 tcaacggcgg cggcgaggtc atcgtgcgcg tgcggacgac cagcggcgac ctgaccgtga
      901 cccccggccc tgagtaccac ggcggctact ccgccgatat gcccgccgcc gaagtctcgg
      961 tcgccccgga ggcggacgcg ggcgacgaga cgcgagcctt cacggacgac gaagccactg
     1021 cggcacgggt cgccatgcag ggcgacggcg gcggggtcga acccggccag acacagcgcc
     1081 tcgacgcgca cgccgaggat cggtccggcc cccgcaagtc ggcggagatg accatcctcg
     1141 acgccatcga gaacggcgaa atgtcggtgg acgagggcct gcgccggctg aacgagctgg
     1201 cgcagtaagc gccggcacgg cctcccggcc agggaggatc gggggcgccc ccgcggggcg
     1261 ccccacctgt ttcccccgtc ctctcgtaga aaaggagtac ccccatgccg gtaaccaccg
     1321 tgcgtatcga gccgctggat ccccatgcca ttaccgagga ccagatcgcc gccgtgcatg
     1381 tactcatgtc gcgcagcgag gccgagcgct tccccaacga cccgccgcgc ccgctggagc
     1441 agacagcggc gctcttccgc aatattcccc cattcttcga cctgcgtatc tgggccgccg
     1501 ggaccgaaga tcggagcacc ctcctgggct atggcatact ggttatgccg cgcaccgagg
     1561 agaaccagca tgccgcgcag attgagatgg aggtgctgcc ggaggcgcgc cgccacggac
     1621 tggccaagcg cctgctgatc ccgatagtcg acgccgccgc gcaggccggg cgccgcctgc
     1681 tcatcgcgaa caccaacagc aatcagccgg gcggggccgc gttgatggag cgcctgggcg
     1741 ccggggccgc gctggtgggg cacatcaatc agcttgtcgt ggccggcgta gaccgcgcca
     1801 tgctgcgcgc gtggcaggag catgctgaga cgcacgcccc gaacttcacc ctgggcctgt
     1861 gggaaggcgc ctacccggac agccacctgg acgagattgc cgccctgttc caggcgatga
     1921 acctggcccc gcgcggcgac ctggacgtgg aagacttccg ctggaccccg gagcagatgc
     1981 gccagatcca ggacatgaag atcgggcgcg gcggcatccc ctggacaatg tatctgcgcg
     2041 acagcaccac gggcgaactg gccgggttca ccgagatcaa ctggaacccc agccagccgg
     2101 gagtcgtctg gcagggtggc acggccgtct ggccgaagta ccgcaaccag ggcctgggcc
     2161 ggcgcctgaa ggcggcgatg ctggagaagg tgctgcggga ccgcccgcaa gtcgaataca
     2221 tccgcaccga gaacgccgac tccaacgcgg cgatgctcaa gatcaaccag gaactgggat
     2281 tccggcccta ctactcggag acgacgtggc aggtcccggt cgatcaggtc cgcgcttacc
     2341 tggggacaac ctaacccctg gccaacctaa ccccccggcc cccttcccgc cgcgggaagg
     2401 gggagacgta tggatccgca cgagggacgt ggggtagggg ccggtttcaa accggcccgt
     2461 gcgacggcca cacccgctgt aggggccgat tgcaaaccgg ctcggcgaca agcccgctgt
     2521 aggggccgat tgcaaaccgg ctcggcgaca agcccgctgt aggagccggt tgcaaaccgg
     2581 cccgccccgc aggacggcgg caataaggag ccttcaggat gatcgcagta ttacgtcaac
     2641 gcaactttgc tctgctctgg ttcgggggcc tgatctccat ggcgggcgat tgggtcttgc
     2701 ggatcggcct gccgctctac atctaccagg tgaccggctc gacgctggcg accgggatca
     2761 tgttcatggc cgcgatgctg cccagcctgc tgctggggtc ggtggccggg gtcttcgtgg
     2821 accgctggga ccgcaagcag accatggtga tcgccaacgt gctgctggcg gggagcctgc
     2881 tgccgctgct gctggtgcgc tcggcggagt gggtctggat cgcgtatatc gccgcctttg
     2941 cccagtcgtg catcgcgcaa ttcttcgacc ccgccgagtc ggccatgctg ccgctgctgg
     3001 tgggcgagga acacctgccg acagccaacg cgctcaacgc gttgaacaac aacctggccc
     3061 ggctgatcgg gccggccatc ggcggcgtgg tgctggcggt gggcgggctg accggagtgg
     3121 ccctggtcga tgccgcatcg ttcctgatcg cggcggggat gatcgcgctg atcacggcca
     3181 atggccgcgc cgtgcgcgcc gagacgcccg ctgccgccga gggcgccggg gggcgctggc
     3241 tggccgtctg gcgcgagtgg gtggaggggc tgcgcatcgt ggtccagacg cgcaccgtcg
     3301 ccattatctt cctcttgctg gggatcacgt cgctgggcga aggggtgatc tcggtgctgt
     3361 ttgccccgtt cgtgagcaaa gtcctcggcg ggggcgggac cgagttcggc gccatcgcca
     3421 gcgcgcaggc cgtcggcggg ctgatcggcg gcgcgctgat cggcgaggtg gcacggcgcg
     3481 tgcggcccgg ccggctcatc ggcgtgtgcg gcatcctgtt cgggctgatc gatctggcga
     3541 tcttcaacta ccccgtcttc ggcgccgccg tgatcctcat ggtgctggtc gggctgccgg
     3601 gcgtcggctt cagcacgggc gtgacgacgc tgctgcaaac aagcacggcc aaccagtacc
     3661 tggggcgcgt gttcggcgcg ctcggcacca cggcgtcctt gctggcgctg atcggcgcgg
     3721 ggctggcctc ggtgctgggc gaccggatcg gcatcgtgcc ggtgcttaac ctccaaggcg
     3781 cggggtatat cctggccgga gccctggcgc tggtgctgct ggcgcacgtg gcgctgccgc
     3841 gcgcgcaggc gccggcggaa gaaagcgtac ccgcgtagat ggatagcggg ccggattgca
     3901 acccggcccc tacaccgggc attgggcctt acatggtaga attagcgaga tccgttcgta
     3961 ggtccgcggg cggagagaaa tcggtctcac gatgtatgga acacgaagcg ggcatccgcg
     4021 tatactacta ttccgcctcg tttcgtcgct gcccaacgcc aagctaacta aaggagccag
     4081 ggtatgtcgt atgaaccgcc gcccaacgga ttccgcacct tcctgatcgt ctggtgcacg
     4141 cagtcagtct ccgtcttcgg cagcgccctc accttcttcg ccgtgacgat ctggctgacg
     4201 caggtgctct accccgatcc ggcgcaaaag cccgaactag cggcggcgct ggccgccgtg
     4261 agcctggcct tctccctgcc cacgctgttc atcgcgccca tcgccggggc ctgggccgac
     4321 cggcacgacc gcaagcacat catgatggcg ggggacgcca ccaacggcac cctcagcctg
     4381 gtgctgatgg cgctcatggc gacgggcagc ctgcaactct ggtcgctgct gctgatcacg
     4441 gtgctgatgg cgcttgccgg ctccttccac ggcgcggcct tcgacacatc gtattctatg
     4501 ctggtgccgg agaagcaact gccgcgcgcc aacggcatga tgcagacgat gtggtcgctc
     4561 tccggcatcc tctccccggc catcgccgcg ggcatcatct cgctgcccgc gctggcccgc
     4621 caggggttgc tgcccttcga ccttaatggg gcgctggcgg agctgaagga cggcacgccg
     4681 ctcgccatca ccatcgacgc gatcacgttc ttcgcggcgg gtgccacgct gctgttcctg
     4741 cgcatcccct cgccggttcg gaccgacctg cagaaagagg ccggcggccc gcgcaagagc
     4801 ctgtggtccg acgtgcgcga gggcgcgctc tatatctggc accgcaagcc cctgctctgg
     4861 ctgctcggca ccttcaccgt cgccaatttc gccggggggc cggtggaagt gttcatgccg
     4921 ctgctgctca agttcaacct ggccgccgac tgggcggcgc gggggttcac ctttgagacg
     4981 gcgctggccc tgatggggtc ggtggcgagt atcggcgggg tgctgggcgg gctactcgtc
     5041 agcgcctggg gcggcctgaa aaagcggcgc atctacggcg tgctggtgcc gatgatcatc
     5101 ggcggcgtgg cacagatcgc cttcggcttc tcgccgctgc tcttcctctc ggcggcgatg
     5161 gccggggtcg tcaccggcat ggtccccatc ctgaacgccc attcgcagtc gatctggcag
     5221 acgcagacgc cgcgcgagtt gcagggacgg gtgttctcgg tgcggcgggt gatcgcccag
     5281 tgcacctggc cgctgagtac cgcgctggcc gggttggccg ggggcttctt caatcccggc
     5341 ctcgtcatcg ccgcgctggg cgcgttcctg ctgatcttct gcgtggcgca gttgttcaac
     5401 ccctatctgc ggcgcgtcga agataaggac tggctcgatt ccctggccgc tcggcatggc
     5461 cccgccgccc cgcctgaacc ggctaccgct atactggaga cggcggacaa aacatgaccg
     5521 tggtcggcgc attacggcaa cgatccttcg cctggctctg gacgggccag gcgatttcgc
     5581 gcctgggcga cagcatccac cgggtcgcgc tctcgtggtg ggtgctggag cagaccgggt
     5641 cggcggcggt gatgggcacc gtgctcatct tctcgttcac gccgatgctg ctgttcctgc
     5701 tgatcggcgg ggtggccggg gatcgcctgc cgcgggtgcg gctgatgctg gggtcggacc
     5761 tgttgcgcgg cggcgtggtg gcgctggtcg cggcgctggc ggcgacccac acgttgcaga
     5821 tctggcatct gtacgtcgcg agcatggtgt ttgggctgct ggacgccttc ttccagcccg
     5881 cctacacggc gctggtgccc gagatcaccc cggcgccctt gctgcccagc gccaactcgc
     5941 tgaccagcct cagcggccaa ctgagcaaca tcgccgggcc ggccatcggc gcggggatcg
     6001 tggccgcggg cggcacggcg ctggcctttg ccgtggacag cctgtcgttc ttcctctcgg
     6061 cggcctgcct catcccgctg atcgggctgc cccaggcacc ggcccccgcc gggcacggcg
     6121 cacgccacat gctgcgcgac ctgcgcgaag gggtgcgcgc cgtgctggcc gcgccgtggc
     6181 tctgggtcac gatcgcgctg gcggcgctgt tcaacatcac ccagggcggg ccgctggcgg
     6241 tggccttgcc cttcctggtg aaagacagcc tgcacgccga cgtgggcgtg ctgggcctgc
     6301 tctactcgat gacctcggtg ggggcggtgc tgagcgcgat ctggctgggc cgggcgcggc
     6361 gcatccggcg gcgcggcctg ctggcctatc tcggcctgct ggcgggcggc ctgaccacgc
     6421 tcatgctcgg cctgccggtg ccgctgccgg cggtggccgt ggtcatcctg ctgaacggcg
     6481 ccgcggtcac ggtcttcggc ctgatctgga ccaacacgct gcaagagttg gtgccgcgcg
     6541 acctgctggg ccgtgtggcg agcatcgaca tgctcggctc gttcgccctg ctgccgattg
     6601 gctacggcat cgccgggtgg gccaccgacc tgctcggccc ggcgctcgtc ttcatcacgg
     6661 gcggcgcgct gacctgcgcg ctggtgctga tcggcctcgc gcaccctggt atccgcaacc
     6721 tggactagcg cgcagcgcat ctctggcgaa ctacaccgca aagcgccctg gcccgattgt
     6781 gttgagccgc gggccggttt caaaccggcc cctacaccgc cagtcgggca acgagccgac
     6841 ccctaccctg taatgcaacg aggccgatta ttcagcaaag caacgggccg cccggcagtt
     6901 tccgggcggc ccgtcgggtt atatgcaggt agcagagtta cgcggtgcgc gccgtggcgg
     6961 tgtcggcggt cttgccgcgc ccgaaccagt tgcgccactc cttgttctcc cagaccacca
     7021 gcatcatgct ggcgttgaag atcgaggagt acgtcccgga gacgatgccg atcagcaggg
     7081 ccaggacaaa gtcgcggatg gacaccccgc cgaacaggaa cagcgccgag agcgtgaaga
     7141 tgaccgtcag cgaggtgttg atcgaccggg tgagggtctg caccaggctg tagttcacga
     7201 ccttctcgaa gctgtcgaag cgccgcttga gcaggttctc gcggatacgg tcgaacacca
     7261 caatcgtgtc gtggaccgag aagccgataa ccgtcagcat ggcggtgatg aacagggcgt
     7321 cgacctcgat accgaacagt tcgcccagga tcgagaagat gccgacgacc acgagcacgt
     7381 catgcagcag cgcgatcacg gcgcagacgc catagcggat cgcgttcggc acccggcgga
     7441 aagcgtacca gagataggcc aggatgccca gcgatgcggc cagcaccgag aggatagcgc
     7501 ggctcgccac ttcggacccg atggtcgggc cgacggtatt gaactggatt tcgtcgcccg
     7561 gtgcgatgcg ggtcacggtg gtggtctcca cagcgggcgc ggtggtcccg gtggtcgtgg
     7621 cggtggcggt gacgggcgcg gtgctcgaca ccgaaacagt ctgctcggtc ccggagatga
     7681 tgccgcggtc caccagcacc ttggtcagcg cgtccttctc atcgggcgag atgttgggca
     7741 tgcgcatgat cagggtgcgc gtggtgacgt tgccgacgat caggttcgcc tcttgcacgg
     7801 cgacatcagt ctgcccgttg tccttgagca actgggccac ttcgttggtg ttgacctcgg
     7861 cgctgcgcgg cacgacttcc cagagggtgc cgccggtgaa gtcgatgccc agcctcagcc
     7921 cgaagaggaa caacgaaatc acgcccggaa taatgataat cagcgaaacc aggaagaaga
     7981 ggtaacgctt accgacaaga tcgaacataa gagagttagc tcctgtacta gcagaccggt
     8041 ggcgggccac cgcgagcaat caaacggtgc aggcgaggcg ggcgccgccg caagggcgcc
     8101 cgcctccatc acagttagcg aaccggctgc gtgcccccct cgggcggcag cgtcgggcgg
     8161 gccgttgact ccaccccgaa ccaccagggg ttgcgcgcca tgcgcgtcga gacgaccagc
     8221 cgcaacaggg tccgcgtcac ggtgatcgca gtgaacaacg acaccagcac ggagatgagc
     8281 agggtcaagg caaagccctt gatcacgctg gcgccgaaga agttgccgaa ccagtacaag
     8341 atgagcgtga tgatcacggt ggtcatattg gagtcgcgga tggagggcca ggcgtgatcg
     8401 aagccggcct ccacggcggc ggccaccgtt ttgccgctgc gtaactcttc tttgaggcgg
     8461 gcgaagatca gcacgttggc gtctacggcc atgcctatgg agaggatgaa gccggcgata
     8521 ccggccagcg tcagcgtcac cgggacggcg cggaagatgg cgaacgtgat gcaggcgaaa
     8581 atcagcagcg cgatgtcggc cagcagaccg ggcagatgat agtagagaag catgaacagc
     8641 atgaccaggc tcaagccgat gatcccggcg atgaggcttt ggtccac
//
LOCUS       DASUYC010000690         2963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1797374, whole genome shotgun sequence.
ACCESSION   DASUYC010000690 DASUYC010000000
VERSION     DASUYC010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1797374"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1204
                     /locus_tag="VF276_11325"
     CDS             <1..1204
                     /locus_tag="VF276_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016362563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6607494.1"
                     /translation="RGVAGAYWTRVRQASAEFFGFLGEHLAGTEDTRANGATGYVLRR
                     FHELIQDWWPVDRRASLAGYSMWMTTLGVFAVGTALAFGLGAYLWSVGAISLGTVYII
                     FNYTELLSRPIEQIRTQLEDLQRADAGIARVEELRAIRSRLADGAGAALPAGPLGVAF
                     DDVTFGYEAGDPVLRDLSFDLPPGTVLGLLGRTGSGKTTLARLLLRLYDPTTGQIRLG
                     GVPIAGAHLRDVRRQVGLVTQDVQLFQASVRDNLTFFNRAIPDAQILAVLDDLGLRGW
                     YDSLPAGLDTELDAGGGGLSAGEAQLLAFVRIFLADPGLVILDEASSRLDPATEQLIE
                     RAVSKLLRDRTGIIIAHRLGTIGRADRIMILEDGRILEQGDRRQLADDPRSRFSRLLQ
                     TGLEEVLA"
     gene            1201..>2963
                     /locus_tag="VF276_11330"
     CDS             1201..>2963
                     /locus_tag="VF276_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007130086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6607495.1"
                     /translation="MRTYQFMWRMARYRPGLYLIDGIFWTLIHLSPLVPGLIAKAFFD
                     SLDRATPGQFNVWWVVAWLVMTALARVLLIVGGALADIRHRFTMSAWLRRNLLARVLE
                     RPGARSLPEAPGEAISRFRDDAERAEDALSWTLDTIGQALFALVAMVILVGINAWITV
                     LVFVPLIGVVAIAQAASTRLEHYRKSSSQATGRVTGALSEIFGAVQAVQVAGAEDHVL
                     AHVRRLSDTRRQLMLKDRVLSQLLDSVFANTVNLGTGLILILAAQSMRGGSFTVGDFA
                     LFVYYLTFVTEFTQFFGMFLAHYRQTRVAFDRMMVLLQGAPPARLVEPSPLPLSGPLP
                     AVVYPPHTPAHRLHMLEATGLTYRYPESGRGIANVDLRLERGSFTVITGRIGAGKTTL
                     VRALLGLLPAEAGTIRWNGQPVADPANFFVPPRSAYTPQVPRLFSASLRENILLGFDV
                     DDAAVRAAVRAAVLDGDLAAMEQGLDTLVGSRGVRLSGGQVQRAAAARMFVRDPELLV
                     FDDLSSALDVETERRLWDRLFERQGATCLVVSHRRAALRRADHIIVLKDGRVEDAGTL
                     DELLGRCAEMQRLWHGELMES"
BASE COUNT          395 a         1031 c         1059 g          478 t
ORIGIN      
        1 ccgtggcgtg gccggggcct actggacgcg ggtgcgccag gccagcgccg agttcttcgg
       61 cttcctgggc gagcacctgg cgggcaccga ggacacgcgg gccaacggcg cgaccggcta
      121 tgtgctgcgc cgcttccacg aactgatcca ggattggtgg ccggtggacc ggcgcgcgag
      181 cctggccggg tacagcatgt ggatgaccac gctgggcgtg ttcgccgtgg gcacggcgct
      241 ggccttcggg ctgggcgctt atctgtggtc ggtcggcgcg atcagcctgg gcaccgtata
      301 tatcatcttc aactacaccg aactgctcag ccgccccatc gagcagatcc gcacccagtt
      361 ggaagacttg cagcgcgccg acgccgggat cgcccgcgtt gaggagttgc gcgcgatccg
      421 ctcgcggctg gccgacggcg ccggggcggc cctgcccgcc gggccgctgg gcgtggcctt
      481 cgacgacgtg accttcggct acgaggccgg cgacccggtg ctgcgcgacc tcagcttcga
      541 cctgccgccg ggcacggtgc tggggttact gggccgcacg ggcagcggca agacgacgct
      601 ggcccgcctg ctgctgcggc tctacgaccc gaccaccggg cagatccgcc tgggcggcgt
      661 gcccatcgcc ggggcgcacc tgcgcgacgt gcggcggcag gtcggcctgg tcacgcagga
      721 cgtgcaactg ttccaggcca gcgtgcgcga caacctgacc ttcttcaacc gcgccatccc
      781 tgacgcgcag atcctggccg tactggacga cctgggcctg cgcggctggt acgactccct
      841 gcccgcgggc ctggatacgg aactggacgc aggcggtggc gggctgtcgg cgggcgaggc
      901 gcaactgctg gccttcgtgc gcatcttcct ggccgaccct ggcctggtca tcctggacga
      961 agcttcgtcg cggctcgacc cggccaccga gcaactgatc gagcgtgcgg tgagcaaact
     1021 gctgcgcgac cgcaccggca tcatcatcgc ccaccggctg ggcacgattg gccgcgccga
     1081 ccgcatcatg atcctggagg atggccgcat cctcgaacag ggcgaccggc gccagctggc
     1141 ggacgacccg cgctcgcggt tcagccgcct gctgcaaacc ggcctggagg aagtgctggc
     1201 gtgagaacct accagtttat gtggcgcatg gcgcgctacc ggcccggcct ctatctgatc
     1261 gacggcatct tctggacgct gatccacctg tcgccgctgg tgcccggcct gatcgcaaag
     1321 gctttcttcg actcgctgga ccgggcgacg ccggggcaat tcaacgtctg gtgggtggtg
     1381 gcctggctgg tcatgaccgc gctggcccgc gtgttgctga tcgtgggcgg cgcgctggcc
     1441 gatatccgcc accgcttcac catgagcgcg tggctgcggc gcaacctgct ggcccgcgtg
     1501 ctggagcgcc ccggcgcgcg ctcgctgccc gaggcgccgg gcgaggcgat cagccgcttc
     1561 cgcgacgacg ccgagcgtgc cgaagacgcc ctcagttgga cgctggacac gatcggtcag
     1621 gcgctgtttg ccctggtcgc catggtgatc ctggtcggca tcaacgcctg gatcacggtg
     1681 ctggtgttcg tgccgctgat tggcgtggtc gccatcgcgc aggccgcgag cacccggttg
     1741 gagcactacc gcaagtcgag cagccaggcc accgggcggg tgacgggcgc gctgagcgag
     1801 atcttcggcg cggtgcaggc cgtccaggtg gcgggcgccg aggaccacgt gctggcccac
     1861 gtgcggcgcc tgagcgatac ccgccgccag ttgatgctca aggaccgcgt gctcagccag
     1921 ttgctcgact cggtattcgc caacacggtg aacctgggca ccggcctgat cctgatcctg
     1981 gccgcccagt cgatgcgcgg cggcagcttc accgtgggcg acttcgccct gttcgtctat
     2041 tacctgacct tcgtgaccga gttcacgcag ttcttcggca tgttcctggc ccactaccgg
     2101 cagacccgcg tggcgttcga ccgcatgatg gtgctgttgc agggcgcgcc gcccgcccgg
     2161 ctggtcgagc ccagcccgct gccgttgagc gggccgctgc ccgccgtcgt ctacccgccg
     2221 cacacacccg cgcaccggct gcacatgctg gaagccaccg gcctgaccta ccgttacccg
     2281 gagagcgggc ggggcatcgc caatgtggac ctacggctgg agcgcgggtc gtttacggtg
     2341 atcaccgggc gcatcggcgc gggcaagacg acgctggtgc gggcgctgct cggcctgctg
     2401 cccgccgagg ccgggacgat ccgctggaac ggccaaccgg tggccgatcc ggcgaatttc
     2461 ttcgtgccgc cgcgcagcgc ctacacgccg caggtgccgc gcctgttcag cgcatcgctg
     2521 cgcgagaaca tcctgctggg cttcgacgtg gacgacgcgg cggtgcgggc cgccgtgcgc
     2581 gctgccgtgc tcgacggcga cctggcggcg atggagcagg gcctggacac cctcgtcggg
     2641 tcgcgcgggg tgcggctgtc gggcgggcag gtgcagcggg cggcggcggc ccgcatgttt
     2701 gtccgcgacc ccgaactgct ggttttcgac gacctttcca gcgcgctcga tgtcgagacc
     2761 gagcgtcgcc tctgggatcg tctgttcgag cgccagggcg ccacctgcct ggtcgtctcc
     2821 catcgccggg cggccctgcg ccgcgccgac cacatcatcg tgctcaaaga cgggcgggtc
     2881 gaggacgcgg gcacgctgga cgagttactg gggcgttgcg cggagatgca gcgcctctgg
     2941 cacggcgaac tgatggaatc gga
//
LOCUS       DASUYC010000691         2677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_123387,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000691 DASUYC010000000
VERSION     DASUYC010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123387"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1265
                     /locus_tag="VF276_11335"
     CDS             <1..1265
                     /locus_tag="VF276_11335"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX6607496.1"
                     /translation="GMAYVLVPAPWRLGVVRRLRSYGLGASRAVVHLPDSAATRYLVP
                     LKAKLLAYTCSTLVDAPSWRLRVLALMLHCPGSADLLALVLPHTGLIVRRAAPPPLLQ
                     WLLHMPPAGHLSGDAILQVTARRPAQSVIIHGFARGGRSPAIIAKVVPAGMEGSNRPG
                     EDAALAELGPAARVAGVAVPQPLLCAMVAGRRVLLQTAVPGRSLAPLLVAHPARFPDY
                     TARVVAWLAQWNRRTLVIQPRSREQLERDVVAPASLLGPLLDSGGAYINWLRRICAAA
                     AGPMPLAAAHNDLTMWNILIDKRGCLGVLDWESARPTDLPLKDFFYALVDAVAATGGY
                     TDRLAAFQQCFAHNGTYRPWLNELQARFPYTRDIPAPVLDLCFHACWIQHAVNEQRSA
                     APHEQRPFLRIVQWLASHTSTVGGRFEL"
     gene            1262..2515
                     /locus_tag="VF276_11340"
     CDS             1262..2515
                     /locus_tag="VF276_11340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6607497.1"
                     /translation="MSETPGSRLRLLFLLPFPPRTDARDGGGRATAQLLLGLAARHDV
                     ALLCLRAAHELPTDPRLGALCRYVEEVDRAAAEDDGWRGRGQLALALLRGEPIWVRSA
                     AVPLFARRVRELARDSHPDVITFQYHVMAQYLPVLNGCPAPRVLTQYEPGTSAAHDLW
                     RSGQGQGRFVPYLDMMAWRRYERRIMRQVQVVVALTRRDQDALARVSGRTSIACIPLG
                     TEIPARALDPRGEVPPSMTFVGNFAHPPNVDAAIRLIDSILPRVWARFPDLTLYVVGD
                     QPPAALQRRTGPHVQVTGRVADVTPYLDRAAVVPVPLRFGGGMRVKVLEALAAGKAVV
                     ASSLAVEGLDVVPGQQVVLAETDEQFAAAIGHLLVDPERRAQLAGRARTWACANLLWD
                     SHVRAYEDLYLHLLSGRTSGAQIHG"
BASE COUNT          379 a          885 c          891 g          522 t
ORIGIN      
        1 aggggatggc ctacgtgctg gtgcctgcgc cctggcgcct tggggtcgtg cgccggctgc
       61 gctcctacgg tttgggcgcc agccgggcgg tggtgcacct gcctgattca gcggcgaccc
      121 gctatcttgt gccgcttaag gccaagctcc tggcttacac ttgttcgacg ctggtggatg
      181 cgccctcctg gcggctgcgt gtcctggcgc tcatgctaca ttgtccgggt agcgccgatc
      241 tcctcgcgct cgtcttgccg cacacagggc tcatcgtacg ccgcgcggcg cctccgcctt
      301 tgctgcaatg gttgttgcat atgcccccag cggggcacct gtcgggagat gccatcctcc
      361 aagtaacggc ccggcgcccc gcccaatcgg tcatcattca cggttttgcg cgcgggggtc
      421 gctcccctgc catcatagcg aaagtggttc cggcgggtat ggaagggtcg aatcgccccg
      481 gcgaagatgc cgcgctggcg gaacttgggc cggccgcgcg ggtggccggg gtggctgtgc
      541 cgcagcctct gctttgtgcg atggttgccg gccggcgcgt gctgttacaa acggctgtgc
      601 ccggccggtc gctggccccg ctactggtcg cccaccctgc tcggtttccc gactatacag
      661 cgcgcgtggt cgcgtggctc gcgcagtgga accggcgaac cctggtcatt caaccccgca
      721 gccgggagca actggagcgg gacgtagtcg ctccagccag cctgctcggg ccgctcctgg
      781 atagcggcgg cgcatacatc aattggctgc gccgcatatg tgctgctgcg gccggcccga
      841 tgccgctggc tgcggcccat aatgacctga ccatgtggaa catcctcata gacaaacggg
      901 gatgtctcgg cgtgctcgat tgggaaagcg cgcgccccac cgatctgccg ctcaaggatt
      961 tcttttatgc cctggtcgat gccgtcgcgg caaccggcgg ttacacggat cgacttgctg
     1021 cttttcaaca gtgctttgcc cataacggca cctaccggcc atggctcaac gagttgcaag
     1081 cgcggttccc gtacacccgc gacataccag cgccggtcct ggatctatgc ttccatgcct
     1141 gctggatcca acacgcagtg aatgagcagc ggtccgccgc cccgcatgaa caacggcctt
     1201 tcctcaggat tgtgcagtgg ctggcgtcgc acacttccac ggtcggaggg cgtttcgagc
     1261 tatgagcgaa acgcccggct cccggctgcg gctgctgttc ctgctcccgt ttcctccgcg
     1321 taccgatgcg cgcgacggcg gggggcgtgc gacggcacag ctcttgttgg ggctcgccgc
     1381 gcggcacgat gtggcgctgc tatgcttgcg tgctgctcat gaacttccga ccgacccgcg
     1441 gctcggcgcg ctgtgccggt acgttgagga ggtggatcgg gccgcagccg aggacgatgg
     1501 gtggcgcggt cggggccaac tcgctctcgc tttactgcgg ggcgaaccga tatgggtgcg
     1561 ctctgccgca gtacccttat tcgcaaggcg agtccgggaa ctggcgcgtg attcgcatcc
     1621 cgacgtcatc accttccagt accatgtcat ggctcagtat ctgcctgtgc tgaatggatg
     1681 ccccgccccc cgcgtgctga cccaatacga gccgggtacc tccgccgcgc atgacctatg
     1741 gcgctcgggg caggggcaag gacggttcgt cccttacctg gacatgatgg cctggcggcg
     1801 ctacgaacgg cgcataatgc gccaggtgca ggtcgtggtg gcacttaccc ggcgggacca
     1861 ggacgccctg gcgcgggtca gcggccgcac gtccatcgca tgcatcccgc taggcaccga
     1921 gattccggcg cgcgcgctcg atccccgtgg cgaagtaccg cccagcatga ccttcgtagg
     1981 caattttgcc caccccccga atgtagacgc cgcaatccgg ctcatcgatt cgatattgcc
     2041 tcgggtatgg gcgcgcttcc cggacctcac tttgtacgtc gtcggcgatc agccaccggc
     2101 ggcgctgcag cgccgcaccg gtcctcacgt gcaggtcacc gggcgggttg ctgatgttac
     2161 cccctacctc gaccgcgccg cagttgtgcc ggtgccgctg cggttcggag gcgggatgcg
     2221 ggtgaaggtc cttgaggcgc tcgccgcagg gaaggccgtc gtcgcctcgt ccctggccgt
     2281 cgagggcctg gatgtagtgc ctgggcagca ggtggtgctg gcggagaccg acgagcagtt
     2341 cgctgcggcc atcggtcacc tgcttgtgga cccggaacgg cgcgcgcagt tggcgggccg
     2401 ggcgcgcacc tgggcctgcg ccaaccttct ctgggatagc cacgtgcggg cttacgaaga
     2461 tctctacctc catttgctgt ccggtcgcac gtctggagcg cagatccatg gataaactga
     2521 aacatctcag cgcagccccg gcgatccggg cggggtatga tttaaatctg cgccgccacg
     2581 ggcgtgtaat gggcagtggc ggcctcacat cctgggtggc ccgcgcgcgg gcgatcgtgt
     2641 taccgttgat gctgctggcg ctggcgttgt atgtggc
//
LOCUS       DASUYC010000692         2804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_799163,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000692 DASUYC010000000
VERSION     DASUYC010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799163"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="VF276_11345"
     CDS             <1..647
                     /locus_tag="VF276_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017720708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX6607498.1"
                     /translation="PLDLWDAASGRLLRTIQGRPGGINLAGLAFAPDGKSIATGNSDN
                     TASLWDVATGQEIRSFSGHTDILYSVAFSPDGKLLATTSWDKTARLWDVATAQELRRF
                     VGHTAGLNRVAFSPDGKWLATTSNDKTARLWDVATGQELRRFSGHTDAVFSVAFSHDG
                     KLLLTGSQDGTARIWRTDYHDTMRYLCGVLTRDFTTEERTQYGITDQRPTCPGP"
     gene            complement(669..>2804)
                     /locus_tag="VF276_11350"
     CDS             complement(669..>2804)
                     /locus_tag="VF276_11350"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6607499.1"
                     /translation="AFARQEAILREAVAAYGGWAYKQVGDAFQVAFQAAPAALAAALA
                     AQQALAAEPWPPAIGPVRVRMALHTGSAEERGDDYVGPPLYHAHRLLSAGHGGQVLLT
                     ATTQALIRDSLPPGVTLRDLGERHLKDLAHPEHIWQASGLGLPVDFPPLKTLDTRPNN
                     LPMTLTPLIGRATERAHAEALFAQPDVRLLTVTGPPGVGKTRFALQLGADLLPEFADG
                     VWFIALAAVEDADLLTATIAGALGLRQEPGRPLLPSLLDHLRRQQVLLILDNFEQVSE
                     GASLVSDLLGGAPGLKVLVTSRVRLHLRGEKELALVPLALPPADPRPVPDALARIPAV
                     ALFVERAADIQPGFTLTPENAGAVAAICRRLDGLPLAIELAAARSKILAPPALLARLE
                     SRLPLLTGGVGDLAAHQQTLRDTITWSYNLLGVAEQQLFRQLAVFAGGFTLAAAEVVC
                     GGSATGAAATGILDSRVARGLPVPDVLDRLTALLDQSLLQSDPGGEETRFRMLETIRE
                     YAWDRLAASGEAAAIRRRHAAYFQALAEAADAGWQGTRQAIWRQRLAVEHDNLRAALA
                     WMQETAQGEAGARLAGALARFWATHGYLAEGRRWLEWALQAAHPLPVADRARLLDGAS
                     EIAYWQADYAAARAWAEEALALRRTLGYPAPIAEALTNLGRILWREGDRAGAQALLSE
                     SQVLRRASGAAPPSAETQRHPSGDPASGT"
BASE COUNT          432 a         1021 c          949 g          402 t
ORIGIN      
        1 cgccgctaga cctatgggat gcggccagcg gccggcttct ccgcaccatc caggggcggc
       61 caggtggtat aaatttagcg ggcctcgcct ttgcgccaga tggcaagtcc atcgccaccg
      121 gtaattctga taataccgcc agcctctggg acgtggccac cggccaggag atccggagct
      181 tcagcggcca cacggacatc ctttattcgg ttgctttctc gcccgacggc aagctactgg
      241 cgaccactag ctgggacaag acggcgcggc tctgggacgt ggccaccgcc caggagctcc
      301 gccgctttgt gggccacacg gccggcctca accgagtcgc cttctcgccc gacggcaagt
      361 ggctggcgac caccagcaac gacaaaacgg cgcgcctctg ggacgtagcc accggccagg
      421 aactgcgccg cttcagcggt catacagatg cagtcttctc ggtggccttt tcgcatgacg
      481 gcaagctcct gctcaccggc agccaggatg ggaccgcacg catctggcgc accgactatc
      541 acgacacgat gcgctatctg tgcggcgtgc tgacgcgcga cttcactacc gaggagcgca
      601 cgcagtacgg tatcaccgac cagcggccaa cctgcccggg gccgtagccg ttggcgggcc
      661 ggtggttgtc aggtcccgct tgctgggtct ccggaggggt ggcgctgggt ttcggcgctg
      721 ggcggggccg cgccgctcgc ccgccgcagc acctggctct cggataagag tgcctgcgcg
      781 ccggcccggt cgccctcgcg ccagaggatg cgccccaggt tggtgagcgc ctccgcgatg
      841 ggcgccgggt agccgagagt tcgccgcaac gccagcgcct cctcggccca ggcgcgggcg
      901 gcggcgtagt ccgcctgcca gtacgcgatc tcgcttgcgc cgtccagcag gcgggcgcgg
      961 tcggccacgg gcaatgggtg cgcggcctgc agcgcccatt ccagccagcg ccgtccttcg
     1021 gccaaatagc cgtgcgtcgc ccagaaccgg gccagcgcgc ccgccaggcg cgcccccgct
     1081 tccccctgcg ccgtctcctg catccacgcg agcgccgccc gcagattatc atgctccacc
     1141 gccaatcgct gccgccagat ggcctgccgc gtgccctgcc acccggcatc cgccgcctcg
     1201 gccagggcct ggaaatacgc cgcgtggcgc cgccggatcg ccgccgcctc gccactcgcc
     1261 gccaaccgat cccaggcgta ctcgcggatc gtttccagca tacggaagcg ggtctcttcc
     1321 ccccccggat cgctctgcaa caggctctgg tccaatagcg cggtgagccg atccagcaca
     1381 tcggggacag gcaaaccgcg ggcgacacgg ctgtccagta tcccggtggc ggcagcacct
     1441 gtggccgacc cgccacacac gacctccgcc gccgcgagcg tgaagccccc ggcaaacacc
     1501 gccagttgcc ggaataactg ctgctccgcc acgcccagca ggttgtagct ccaggtgatg
     1561 gtatcgcgca acgtctgctg atgggccgcc aggtcgccga cgccgccggt cagcagcggc
     1621 agtcggctct ccaggcgtgc cagcagcgcg ggcggcgcga ggatcttgct ccgcgccgcc
     1681 gccagctcga tcgccagggg cagtccgtcc aggcgccggc agatggccgc gaccgcgccc
     1741 gcattttccg gcgtgagcgt gaagccgggc tggatatcgg ccgcgcgctc gacaaagagg
     1801 gccactgcgg gaatgcgggc cagcgcatcc ggaaccgggc gcggatcggc tggcggcagc
     1861 gcgagcggga ccagcgcgag ttccttctcg ccccgcagat gcagccgcac ccggctcgtg
     1921 accaggacct tcagccccgg cgcgccgccc agcagatcgc tcaccaggct cgccccctcc
     1981 gagacctgct caaagttgtc caggatcagc agcacctgct ggcgccgcaa gtggtccagc
     2041 aggctcggca ggagcgggcg cccgggttct tgtcgcaagc cgagagcccc ggcgatcgtc
     2101 gctgtgagca aatcggcatc ttccaccgcc gccagcgcga tgaaccagac gccatccgcg
     2161 aactcgggca agagatcggc gcccagttgc agcgcgaacc gggtcttgcc gacgccgggc
     2221 ggccccgtta ccgtgagcaa gcgcacgtcg ggctgcgcga acagggcctc cgcgtgcgcc
     2281 cgttccgtgg cgcggccaat cagcggcgta agcgtcatcg gcagattgtt gggccgggtg
     2341 tccagggttt tcaggggcgg gaaatcgacg gggaggccca gtccgctggc ctgccagatg
     2401 tgctccggat gggccagatc tttgaggtgt cgctcaccaa ggtcgcgcag cgtgacgcca
     2461 ggaggcaagc tgtcgcggat cagggcctgc gtcgtcgccg tgagcaggac ctggccgccg
     2521 tgcccggcgc tcagcaagcg atgggcgtgg tagagcgggg ggccgacgta atcgtcgccg
     2581 cgttcctcgg cgctcccggt atgcaaagcc atccgcaccc gcacggggcc gatggccggg
     2641 ggccagggtt cggcggcgag cgcctgctgc gcggccagag cggcggcaag cgctgccggc
     2701 gcagcctgga aggcgacctg aaatgcatcg ccgacctgct tatatgccca acccccatag
     2761 gcggcgacgg cttcccgcag gatggcctcc tggcgcgcaa aggc
//
LOCUS       DASUYC010000693         3241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1492204, whole genome shotgun sequence.
ACCESSION   DASUYC010000693 DASUYC010000000
VERSION     DASUYC010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492204"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            426..665
                     /locus_tag="VF276_11355"
                     /pseudo
     CDS             426..665
                     /locus_tag="VF276_11355"
                     /inference="COORDINATES: protein motif:HMM:NF025881.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
     gene            681..2120
                     /gene="radA"
                     /locus_tag="VF276_11360"
     CDS             681..2120
                     /gene="radA"
                     /locus_tag="VF276_11360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX6607500.1"
                     /translation="MGRAAAQAQIAALSAVQAPSTQRDTIPTGPEFWSRLIAAKGSRT
                     FRELSQQAGVRIANRRHEAPLCRSTVSQLAKVLPDPYFIALGDGDVYWDTITAIEPAG
                     EAAVYDLTVPGPHNFVANDLIVHNSTMLLDVSAQLATGGRKVLYVSAEESIQQIKLRA
                     ERLGLHQPDLYLLGESDLDTVRNHISQMKPGLVVIDSIQTVFLPELPSTAGSVTQVRE
                     STAALMKQAKTSHIPMFLVGHVTKEGNIAGPRVLEHMVDAVLYLEGDRFHQYRLLRSV
                     KNRFGSTNEVGVFEMAAEGLIEVPNPSAAFLAERSSGVSGSTVAVTMEGTRPLLVEVQ
                     ALTTPSATGMPRRTANGIDINRLHLLLAVLTKRMGVPLYNQDVYVNVVGGLKIDEPAV
                     DLAVAVAIASSFKNVQVDPELAVIGEVGLSGELRSVGQLDRRLNEAAKLGFRRAVYPK
                     TGRAPRVDGDIQARAVTDLPDAIEAALTA"
     gene            2309..>3241
                     /locus_tag="VF276_11365"
     CDS             2309..>3241
                     /locus_tag="VF276_11365"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEX6607501.1"
                     /translation="MDEWSRYSRQMVLPGVGRSGQAHLRAARVTILGLGALGSALAES
                     LTRAGVGTLRLIDRDYLELHNLQRQVLYTEADVAAALPKAIAAQAHLQAINGGVTLEP
                     RVVDATTGNIGELIADTDLVLDATDNFAARYLLNDACVQTGRPWIYSAVLGTAGMVMV
                     VRPGQTACLRCLFDTPPPPGASPTCETAGVLGPAVQVVAGLAGAAALQLLLGGDPPRG
                     LLALDVWEGRFDRLGDGAPDPECPACGRREFEFLGGGARAALDGTQLCGRQTVQVRMG
                     GHRIDLDAVAGRWRASAAGQVLANAYLARLSLAPA"
BASE COUNT          580 a         1015 c         1092 g          554 t
ORIGIN      
        1 gagggatcgc cactgcattc gatatgcagc tcgtaattta ccccaaagca aaaactaagg
       61 cgatgcaata ccgcttcgcc aatgacggcc aacaacgccg attgcaccgc cagcaagtgg
      121 cagaggcgat ccgatctgcc cgctccgcta acaacatcag ttgggcagag tgggcgcgcc
      181 gcgccggcgt tgcacacaag aatttgtgga tctggggacg cggtcaagcg gtccccagta
      241 tggaaaacct gcaacgcctt gctggtgcat tggatatccc gctcagtaat ttaaggcccg
      301 aggcgcgaga caaagcagcg atggtgactc ccatagctcg cttgttggag gatgtcggtc
      361 tgcgctataa gaaagccgcg accaaagccg tccctacctg cattttccgg ctcccgcgcg
      421 aagagatggt gatcttcctc aaaaccctgt ttacctgtga tggatccgtc tacattaaca
      481 agcgcggggt tgttggtatc tcgtacagta cgatcagcag acggttggca gaagatgtcc
      541 agcatctgct gttgcgcttt ggcttggtgg cgacgctacg gtcgaagctg ggccaggtta
      601 acgggcagcc gtacacgtcc tatgagattg tgctgctcgg cgtaaacaca gtgcagcaat
      661 ttctcgcgga aatcggcatc atgggtcgcg ctgcggcgca ggcgcaaatc gcggcgctca
      721 gtgcggtgca agccccatca acgcagcgcg acacgatccc gaccggcccc gaattctggt
      781 cgcggcttat agcggccaag ggctctcgca ccttccgcga gctttcgcag caagctggtg
      841 tgcgcatcgc caaccgacgg cacgaggcgc ctctttgccg atccacggtg tcgcagttgg
      901 caaaggtctt gcctgacccc tattttatcg cattaggtga tggagatgta tattgggaca
      961 ccatcacggc cattgaaccg gcgggtgaag cggcggtata tgatctcact gtaccgggac
     1021 cgcataactt tgtggcaaat gacctgattg tccataattc gaccatgctc ctggacgtaa
     1081 gcgcacaact ggcgacgggt gggcggaaag tcctttatgt ctcggcggag gagtcgatcc
     1141 agcagatcaa gctgcgcgcc gagcggctgg gactgcacca acccgacctc tacctgctgg
     1201 gcgagagcga cctggacacg gtgcgcaacc atatcagcca gatgaagcca gggctggtgg
     1261 tgatcgactc gatccagacg gtgttcctgc ccgaactgcc ctccaccgca ggcagcgtga
     1321 cccaggtgcg cgagtcgacc gcggcgctga tgaaacaggc caagacctcg catatcccga
     1381 tgttcctggt cggccacgtg acgaaagagg gcaacatcgc cgggccgcgg gtgctggagc
     1441 acatggtgga cgccgtgctc tacctggaag gcgaccgctt ccaccagtat cgcctgttgc
     1501 gcagcgtcaa gaaccgcttc ggctcaacta atgaggtggg cgtgttcgag atggccgccg
     1561 aggggctgat tgaagtgccg aacccgtcgg ctgcgttcct ggccgagcgc agcagcggcg
     1621 tctccgggtc cacggtggcc gtgaccatgg agggcacgcg cccgctgctg gtggaggtgc
     1681 aggcgctgac gacgcccagc gccaccggga tgccgcgccg gacggccaac ggcatcgaca
     1741 tcaaccgcct gcacctgctg ctggccgtgt tgaccaagcg catgggcgtg ccgctctata
     1801 accaggacgt gtatgtcaac gtggtcggcg gcctgaagat cgacgagccg gcggtggacc
     1861 tggccgtggc ggttgccatt gcctcctcgt ttaagaacgt gcaggtggac ccggaactgg
     1921 ccgtgatcgg cgaagtgggg ctgtcgggcg aactgcgcag cgtgggccag ctcgaccggc
     1981 ggctgaacga ggcggccaag ctcggcttcc gccgcgccgt ctaccccaag accggccgcg
     2041 ccccgcgcgt ggacggcgac atccaggccc gcgctgtcac cgacctgccc gacgctatcg
     2101 aggcggcgtt gacggcgtag cggtcagcgg tcagcggtca gcggtcggcc gtcaggtgtt
     2161 tcatatggat atgtcattct gagcgcagcg aagaatctcc gccttgtagg cgaaaagacg
     2221 agttccgcat ttgccgcccg gcaacacata ccgcggatgt accctcgcat ctgggacgac
     2281 tcacgcgggc gcagccagga ggatcagcat ggacgaatgg agccggtatt ctcgccagat
     2341 ggtgttgccc ggtgtcgggc ggagcggaca ggcgcacctg cgggcggcac gggtcaccat
     2401 cctggggctc ggtgcgctcg gctcggccct ggccgagagc ctgacgcggg cgggcgtggg
     2461 cacactgcgc ctgatcgacc gggattacct cgaattgcat aatttgcagc gccaggtact
     2521 gtacaccgaa gccgatgtgg cggccgcgtt gcccaaggcc atcgcggccc aggcccatct
     2581 ccaggcgatc aacggcggcg tgaccctgga gccgcgggtg gtagatgcca ccacggggaa
     2641 tatcggcgaa ctgatcgccg atacggacct ggtgctcgat gcgaccgaca acttcgcggc
     2701 gcgctacttg ctcaacgacg cctgcgtgca gaccgggcga ccctggatct acagcgctgt
     2761 gctgggcacc gcgggcatgg tgatggtggt gcgaccgggg cagacggcgt gcctgcgctg
     2821 cctcttcgac accccgccgc cgcccggcgc cagtccgacc tgcgagacag cgggcgtact
     2881 cggccccgcc gtgcaggtgg tagccgggct ggccggagcc gccgcgctgc aactgctgct
     2941 gggcggcgac ccaccacgcg gcctgctggc gctcgatgtg tgggagggcc ggttcgatcg
     3001 gctgggcgac ggcgcacccg atccggagtg cccggcctgc gggcggcggg agttcgagtt
     3061 cctgggcggc ggggcgcgcg ctgcgctcga cggcacccag ctctgcgggc gccagacggt
     3121 gcaggtacgc atgggcggcc accgcatcga cctggatgcc gtggccgggc gctggcgggc
     3181 gagcgccgcc gggcaggtgc tggcgaacgc ctatctggcg cggctcagcc tggccccggc
     3241 c
//
LOCUS       DASUYC010000694         2959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1404710, whole genome shotgun sequence.
ACCESSION   DASUYC010000694 DASUYC010000000
VERSION     DASUYC010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1404710"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1546
                     /locus_tag="VF276_11370"
     CDS             <1..1546
                     /locus_tag="VF276_11370"
                     /inference="COORDINATES: protein motif:HMM:NF019311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HEX6607502.1"
                     /translation="PAEAVLLVLGLALLIRQWRRPAAFLLLLAGFGVVFTGGTLLDAT
                     TIPNFAHWMPALSVVYLALALPLAAGWQAWRRLPRRARMIAGGVLAVALVADAGANLY
                     SYLVVYPLRVPPDHSLEALQGRYLESAGPTARVRIAGLTWQPFNPEIAALMSPQTPGG
                     DLINPSRELPLPGDPAHDLAFLFYNDQMPLLPVVQAYYPGGRTQAVQTPDGNRIATAY
                     RIPAAVAGALYGVQAEMRAARIANRVQWSGRLPGFGALPPDTLAYPVIATWSGAVYLP
                     VGPVRLRVTGDPAAQLWVQGQPVAPNTPVAAEPGWVRVAVQVQLSGPQPLQLLLQIGD
                     APPAAIPATDLWPVPPNQGLATTLTGPGAPVHRIDPFVSARVLQVGPSHDPAPLSLGA
                     RTGGTRGIRWQGEVETGAGSYTMILRGDGGTSLWIDGQLVVNDCAAAPAAHEASAPLV
                     LTAGWHRVQLDLQPGSGTGGVEWHWVRPDGVREVVPPGALRTGPDAGPGAPIVWPDPL
                     EPIVCR"
     gene            complement(1622..1882)
                     /locus_tag="VF276_11375"
     CDS             complement(1622..1882)
                     /locus_tag="VF276_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607503.1"
                     /translation="MAIQIRLIADNLDELNATIQKLGTRMGDQIELHMPRDGRNGEWI
                     VYGTWTPAAQTAAELARPEASERARPDVLPLRPDDGPWQAAS"
     gene            complement(2013..2225)
                     /locus_tag="VF276_11380"
     CDS             complement(2013..2225)
                     /locus_tag="VF276_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607504.1"
                     /translation="MSNTPWYLWPFVALWRLVAGIIGLTGRLVAVLLGVVLFVAGGLL
                     TLTVVGAIAGIPLMIIGVLLIVRGIF"
     gene            complement(2343..>2959)
                     /locus_tag="VF276_11385"
     CDS             complement(2343..>2959)
                     /locus_tag="VF276_11385"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6607505.1"
                     /translation="KELKYGAGTFWSETPGVVHEAGNNGTGNARVSAAYLLPKDAPLS
                     IPVQGAPTPAIAGKPIYTSKLSLPGIAGDYDFVARVLDFAPGVWTPLHTHPGNVVLTV
                     MEGEFTLREEGKEVKYPAGTSWTEAPGHVHMAGNNGTTNARVSAVYLVPKGAALAETV
                     QAPPVGMPATGGHQGPDGWLPLVLLSLGLLALGGGGHLLRRTVR"
BASE COUNT          402 a         1039 c         1059 g          459 t
ORIGIN      
        1 gcccgcggaa gcggtcttac tggtgctggg cctggccctg ctgatccggc agtggcggcg
       61 gcccgccgcc ttcttgctgt tgctggccgg gttcggcgtc gtgttcacgg gtggcacatt
      121 gctcgacgcc acgaccatcc cgaattttgc ccactggatg cccgcgctct ccgtggtcta
      181 tctggccctc gcgctgccgc tggcggcggg gtggcaggcg tggcgccggc tgccgcgccg
      241 tgcccggatg atcgccgggg gcgtgctggc cgtggccctg gtcgccgacg cgggcgccaa
      301 cctgtacagc tacctggtgg tctacccgct gcgggtgccg ccggaccatt cgttggaagc
      361 cctgcaaggc cgctacctgg agagcgccgg cccaacagcc cgcgtgcgga tcgccggcct
      421 gacctggcag ccgttcaatc ccgagatcgc cgcgctgatg agtccgcaga cgcctggagg
      481 cgacctgatc aaccccagcc gcgaactgcc gctgcccggc gacccggcgc acgatctcgc
      541 gttcctgttc tacaacgacc agatgccgct gctgccggtg gtgcaagcct attatcccgg
      601 cgggcggacc caggcggtgc aaacacccga cggcaaccgt attgccacgg cgtaccgcat
      661 cccggcggcc gtggccgggg cgctgtacgg cgtccaggcc gagatgcggg ccgcccgcat
      721 cgccaaccgg gtgcaatggt cgggccgcct gccgggcttc ggcgcgctgc ccccggatac
      781 cctggcctac ccggtgatcg ccacctggag cggggccgta tatctgcccg tcggcccggt
      841 gcgcctgcgg gtgacgggcg acccggcggc gcaactctgg gtgcagggcc aacccgttgc
      901 accgaatacg cccgtggccg ccgagccggg atgggtgcgc gtggccgtgc aagtccaact
      961 gagcggaccc caacccctgc aactgctgct gcagatcggc gatgcgcccc cggcggcgat
     1021 cccggcgacc gacctctggc ccgtgccgcc caaccagggc ctcgccacga cgctgaccgg
     1081 gccgggcgcg ccggtccacc gcatcgatcc ctttgtcagc gcgcgggtgt tgcaggtcgg
     1141 gccgtcgcac gatccggcgc cgctcagcct gggagcgcgc acgggcggca cccgcgggat
     1201 ccgctggcag ggcgaggtgg aaaccggcgc cggctcgtat acgatgatcc tgcgcgggga
     1261 tgggggtacg agcctgtgga tcgatgggca gcttgtcgtg aacgactgcg cggcggcgcc
     1321 ggccgcgcac gaggcgagcg cgccgctggt cctgaccgcc ggatggcacc gggtgcaact
     1381 ggatctgcaa cccggcagcg gcacgggcgg cgtcgaatgg cactgggtgc ggcctgacgg
     1441 cgtgcgcgag gtcgtgccgc ccggcgcact ccgcaccggc ccggacgccg ggcccggcgc
     1501 gcccatcgtc tggcccgatc cgctggagcc gatcgtctgc cgctagcgtt tcctgaaaat
     1561 atcatcggcc ctggatcgca ataaaccgat ccggggccgt gtctgtcggg cggggtgggc
     1621 gttagctcgc ggcttgccag gggccgtcgt cagggcgcag cggcagcaca tccgggcggg
     1681 cgcgctcgga ggcttcgggg cgggccagtt ccgcggcggt ttgcgcggca ggcgtccagg
     1741 tgccgtagac gatccactcg ccattgcggc cgtcgcgcgg catgtgcagt tcgatctggt
     1801 cgcccatgcg ggtgccgagc ttctggatcg tcgcgttcaa ttcgtcgagg ttatcggcga
     1861 tgagccggat ctggatcgcc atggggctgc tcctcctggg tcgggcggcg aatcggcggc
     1921 gggcaggaat gggcccgcga cgcgggatgc ttcaccttac cattatcgca gaccgcactc
     1981 tgccggcact ctattggcgc tctcaattgg cgctaaaaga tgccgcgcac gatgagcagc
     2041 acgccgataa tcatcagcgg gatgccggcg attgcgccga cgacggtcag cgtcagcagc
     2101 ccgcccgcga cgaacagcac gacgccgagc agcacggcca ccagccgccc ggtcaggccg
     2161 atgatgccgg cgaccagccg ccagagggcc acaaagggcc agagatacca gggtgtgttg
     2221 gacatattct tcccctttcc gccggatgag gcaggcgtag catataaagg gccggacggg
     2281 tcgtgcccat ccggcccact cgatctacgc cccggcgggg ccgggggttg cggggtgcgg
     2341 ggtcagcgca ctgtgcgccg caggaggtgg ccgccgccgc ccagcgccag cagccccagg
     2401 ctcagcagca ccagcgggag ccagccgtcc gggccctggt ggccgccggt cgccggcatg
     2461 cctaccgggg gcgcctgcac ggtttccgcc agggcggcac ccttcggtac caggtacacc
     2521 gccgaaaccc gcgcgttggt agtgccgttg ttgccggcca tatgcacgtg cccaggggct
     2581 tccgtccagg aggtgcccgc ggggtacttc acctctttgc cttcctcgcg cagagtgaat
     2641 tcgccttcca tcaccgtcag gaccacgttg ccggggtggg tgtgcaacgg ggtccagacc
     2701 cccggcgcga agtcgagcac tcgtgcgacg aagtcgtaat cgcccgcgat gccgggcagg
     2761 ctgagcttgc tggtgtagat cggcttgccc gcgatggcgg gggtgggcgc gccctgcacg
     2821 ggtatcgaga gcggggcatc cttcggcagc aggtaggcgg cggacacccg cgcgttaccc
     2881 gtgccgttgt tgcccgcctc atgcacgacc cccggcgtct cgctccagaa ggtgcccgcc
     2941 ccgtacttca gttccttgc
//
LOCUS       DASUYC010000695         4541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_124068,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000695 DASUYC010000000
VERSION     DASUYC010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4541
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124068"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VF276_11390"
     CDS             complement(<1..220)
                     /locus_tag="VF276_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6607506.1"
                     /translation="MRDGVPEPGGVWADLGSGEGAFTLALADLLGPGGRIYSVDRDRG
                     ALDVQARVLRARFPAVAVHPLTADFTRPL"
     gene            complement(244..1689)
                     /locus_tag="VF276_11395"
     CDS             complement(244..1689)
                     /locus_tag="VF276_11395"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX6607507.1"
                     /translation="MQTYEIAPDESMEMLVRTLSRMVRRDKDLVLVIPPGVNPFTGVE
                     SVTKLKGVMRSYDARLQVQAADPAVAALFQEAGIPVSPLTAQALAPPPPQAAAPRPPA
                     GPLPFPAPANPPQPAVQHFVPSDQAPQRGGDLPADIANMNFDLEDVGHPTPHNPAPYV
                     PAPPQKGGFLQKLMGRSTPPTEPAPERPRPNADQPLPQPDWAQSGDGPESADLFGEGP
                     TGLDVTMPEWLRDAPPEPPAAGNRVVGRLPTSNLDVTMPQIGGNAPSAGPGTGDLGVP
                     SWLAEYGQSNPPPAPLGASQEAQGPSAASAMPAWLAEEAGAGPAAPVDTGGDLGGLPE
                     WLRDETPVAPPIQPGPYSAPAPAWPTANAAPSASQAAAPPPALSAPAAPAPAAQPNAG
                     AGLGGPEQTPESIAQAVRAAGLDARTRALLIFGMALVRLAPGEAAAGAAAARQAGCSK
                     EELQLVVEMAQALGGGPSERLGRKILGSQHP"
     gene            complement(1750..3495)
                     /locus_tag="VF276_11400"
     CDS             complement(1750..3495)
                     /locus_tag="VF276_11400"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEX6607508.1"
                     /translation="MKLDSSLIRRTLPIWIILVPALALLARGSDATPPARAADGLFVE
                     VQSSRLVYQGQPVRLKGVSFYPRAQPWAGMWTRWNGPATRADLEPLTAFGANTIRVLV
                     PYREDQGVIDSTGAPTARFLDRARQIVQMVGELQCKVLFTLFDWYDDTPAVTDPRWQK
                     NLIYLRAMTDAFGQDDRVLGWDLHNEPDHYASWTDRQPEALDWLLRVGAELHRLAPRQ
                     LVTVGVGHAATLWQADAQGRTLLDVSDFVSFHSYDAGSIRQDIAAVRAHTSKPVVLEE
                     TGWPTGPCGADPTYNENHQVILYREMVQAAQDAGIDGLLAWTLWDFPPTSSAGAGVES
                     EHDHFGLLRLDGSIKPGAVLFRDGFRAGVPPLPSHTTTTLPLSVAPTPGPVQHPPNWQ
                     PPLVFPQTGFDVWDEFRDYWRRFGGVLIFGYPIGEARMEGGRKVQYFERARFEWHPEN
                     ARLKGYDALDKGSKLRLLVQLTRVGATLADARQFPPAAPPAPGGDVRWFPETQHTLRG
                     QFRQFWEQYAGLTNFGYPLSEEMREVSPANGQTYTVQYFERARFEYHPENAGTPYEVL
                     LGQLGTEMLAARGCR"
     gene            complement(3667..4485)
                     /locus_tag="VF276_11405"
     CDS             complement(3667..4485)
                     /locus_tag="VF276_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6607509.1"
                     /translation="MNPAEGDAGFVGSIPQIYDRYLVPLIFESYAADLAGRVARRRPA
                     RVLELAAGTGVATRALARVLPAGVAITATDLNQPMLDHAAAIGTARPVVWRQADAGQL
                     PFPDADCDVVVCQFAVMFFPDKVRAFAEARRVLRPGGRFLFSVWDRIEDNEFADVITQ
                     ALATLFPDDPPRFLARVPHGYHDPAVIAQDLARAGFAQPPQVVTVAERSRADSPRVPV
                     VAYCQGTPLRGEIEAHGASRLNEATDWATEALARQFGAGPVDGKIQAHIIAVDR"
BASE COUNT          674 a         1533 c         1682 g          652 t
ORIGIN      
        1 cgaggggccg ggtgaagtcg gcggtgaggg gatggacggc cacggcgggg aaccgggcgc
       61 gcagcacgcg ggcctgcacg tcgagcgcgc cgcggtcgcg atccaccgaa tagatgcgcc
      121 cgccgggccc cagcaggtcg gccagggcga gggtgaaggc gccctcgccg gagccgagat
      181 cggcccagac gccgcccggc tccggcacgc cgtcgcgcaa gagcgcaaca tgatcggcgt
      241 gattcagggg tgctggcttc ccagaatctt gcggcccagg cgctcgctgg gcccgccgcc
      301 cagcgcctgg gccatttcga ccaccaactg gagttcttcc ttcgagcagc ccgcctggcg
      361 cgcggcggcg gcgccggcgg cggcctcacc gggcgccagg cggaccaggg ccatgccgaa
      421 aatcaagagc gcgcgggtcc gtgcatcgag gcccgctgcg cggacagcct gcgcgatcga
      481 ctcgggggtt tgctccggcc cgccgaggcc cgcgcctgcg ttcggctggg ccgccggcgc
      541 gggcgccgcg ggcgcggaca gtgcgggcgg cggggccgcc gcctgtgatg cgctcggggc
      601 cgcattggcg gtgggccagg cgggcgcggg cgcgctgtag gggccgggtt ggatcggagg
      661 cgccacgggc gtctcgtcgc gcagccactc cggcaacccg ccgaggtcgc cgccggtgtc
      721 cactggcgcg gcaggcccgg cgcccgcctc ttcggctagc caggcgggca tggcggacgc
      781 ggcggacgga ccctgtgcct cttgagatgc ccctagaggg gcaggcggcg ggttgctctg
      841 gccgtattcg gccagccagg acggcacgcc gaggtcgccg gtgccggggc cggcgctggg
      901 cgcgttcccc ccgatctgag gcatggtcac gtcgaggttg gaggtgggca gccggcccac
      961 gacgcgattg ccggcggcgg gtggctccgg cggcgcgtcg cgcagccatt cgggcatggt
     1021 cacgtcgagg ccggtggggc cttcgccgaa cagatcggcg gattccggac cgtcaccgct
     1081 ctgcgcccaa tcgggctggg gcagcggctg atcggcgttg ggccggggcc gctccggcgc
     1141 cggctcggtg ggcggcgtag accggcccat cagtttttgc aggaagccgc ccttctgcgg
     1201 cggcgcgggc acatagggcg ccgggttgtg gggggtgggg tggcccacat cctccagatc
     1261 gaagttcatg ttggcgatgt cggcgggcag atcgccgccg cgctgcgggg cctgatcgct
     1321 ggggacgaag tgctgaaccg cgggctgggg cggattggcg ggcgccggga agggcagcgg
     1381 gccggcgggg ggccggggcg cggcggcctg cggcggaggc ggcgcgaggg cctgcgcggt
     1441 cagcgggctg accgggatgc ccgcctcctg gaacagcgcg gcgacggcgg ggtcagcggc
     1501 ctgcacttgc aggcgcgcgt catagctgcg catcacccct ttgagcttgg tgacgctctc
     1561 gaccccggtg aaggggttca cgcccggcgg gatcaccagg accaggtctt tgtcccggcg
     1621 gaccatgcgg ctcaacgtcc gcaccagcat ctccatcgat tcatcgggcg cgatttcata
     1681 cgtctgcatg ccaagttcct ccgggatacc gaggccggcg accggctccc cagggcgatg
     1741 aaccccgcgt caacggcagc cgcgtgcagc cagcatctcg gtacctagtt ggcctaagag
     1801 cacctcatag ggggtgcctg cgttttccgg gtgatattca aaacgggcgc gctcaaagta
     1861 ttgtacggta taggtctgac cgttagccgg cgacacctcg cgcatctctt cgctgagcgg
     1921 atagccgaaa ttggtcaggc ccgcgtattg ctcccagaac tgccggaact gcccgcgcaa
     1981 cgtgtgctgg gtttcgggga accagcgcac gtcgccgccg ggcgccgggg gcgcggcggg
     2041 cgggaactgc cgcgcgtcgg ccagcgtcgc gcctacccgc gtcaactgga ccagcagccg
     2101 cagcttgctg cccttatcca gggcgtcgta acccttgagc cgggcattct ccggatgcca
     2161 ctcgaaccgc gcccgttcga aatattggac cttgcggccc ccttccatgc gcgcctcgcc
     2221 gattgggtag ccgaaaatca gcacgccccc gaagcggcgc cagtaatcgc ggaactcatc
     2281 ccagacatcg aagcccgtct gggggaagac cagcggcggc tgccagttgg gcgggtgctg
     2341 caccgggccg ggcgtcggcg ccacgctcaa cggcagcgtg gtggtggtat ggctcggcag
     2401 cggcggcacc ccggcgcgaa agccgtcgcg gaacagcacg gcgcccggct tgatgctgcc
     2461 atcgagccgc agcaggccga aatgatcgtg ttcgctctcc acgcccgcgc ccgcggacga
     2521 ggtcggcggg aagtcccaga gcgtccaggc cagcagcccg tcgatcccgg catcttgcgc
     2581 cgcctggacc atctcgcgat agaggatcac ctggtgattc tcgttgtagg tcgggtcggc
     2641 gccgcagggc ccggtcggcc agcccgtctc ttcgaggaca accggcttgc tggtatgggc
     2701 gcgcacggcg gcaatgtcct ggcggataga cccggcgtca tagctgtgga agctgacaaa
     2761 atccgacacg tcgagcaagg tgcgcccctg cgcgtcggcc tgccagagcg tggcggcatg
     2821 gcccacgccc accgtgacca actggcgcgg ggccagccgg tgcagttcgg cgccgacgcg
     2881 caacagccaa tcgagcgcct ccggctggcg gtcggtccag cttgcataat ggtcgggttc
     2941 gttgtgcagg tcccagccca gcacgcggtc gtcttgcccg aacgcatcgg tcatggcgcg
     3001 caggtagatc aggttcttct gccagcgcgg gtcggtgacg gcgggggtat cgtcgtacca
     3061 gtcgaacagg gtgaacagca ccttgcactg caactcgccc accatctgca cgatctggcg
     3121 cgcccggtcg aggaaacggg ctgtcggggc gccggtggag tcgatcacac cctggtcctc
     3181 gcggtagggc acgagcacgc ggatggtgtt ggcgccgaag gcggtcagcg gttccaggtc
     3241 ggcgcgggtg gcggggccgt tccagcgggt ccacatcccg gcccagggct gggcacgggg
     3301 ataaaacgac acgcctttga gccgcaccgg ctgcccctga tacaccagcc ggctgctctg
     3361 cacttcgacg aacagcccat cggcggcgcg ggccggcggg gtggcgtccg agccacgcgc
     3421 cagcagcgcc agcgcgggca ccaggatgat ccagattggt aaggtgcgac gtatgagaga
     3481 tgagtccagc ttcacgtgtt gggttccgtc ctagcaggcg aacgcagggg gtgcacgtac
     3541 atcaggccgc tacaacgcct tcatcatagc ataaagccgc atccggcaca agacgagtgc
     3601 tgcgggctgc gtagtgacga gtgatgaagt ccggccggtc tcggcggacc ggcatggttg
     3661 cgggtgctag cggtcgacgg cgatgatgtg ggcctggatc ttcccgtcca ccggcccggc
     3721 gccgaactgc cgcgcgaggg cctcggtggc ccagtcggtg gcctcgttga ggcgggatgc
     3781 gccgtgcgcc tcaatctcgc cgcgcagggg cgtgccctgg caataggcca cgaccggcac
     3841 gcgcggggag tccgcgcgac tgcgctcggc caccgtaacc acctgcggcg gctgtgcgaa
     3901 cccggcgcgt gccagatcct gggcgatgac ggccgggtca tggtagccgt gcggcacgcg
     3961 ggccaggaag cgcgggggat cgtcggggaa cagcgtcgcc agcgcctggg tgatgacatc
     4021 ggcaaactcg ttgtcctcga tgcggtccca tacactgaag aggaaccggc cgccgggccg
     4081 cagcacgcgc cgcgcttccg cgaaggcccg caccttgtcg gggaagaaca tgaccgcgaa
     4141 ctggcagaca accacatcgc aatcggcatc ggggaacggc aactgcccgg catcggcctg
     4201 gcgccacacg acgggccgcg ccgtgccgat tgccgccgca tgatcgagca tcggctggtt
     4261 cagatccgtc gccgtaatgg cgacccccgc cggcagcacc cgcgccagcg cgcgggtcgc
     4321 cacgccggtg cccgccgcca gttccagcac gcgcgcaggc cggcgccggg ccacgcggcc
     4381 cgcgagatcc gccgcgtagg actcgaaaat gagcggcacc aggtaccggt cgtagatctg
     4441 gggaatggac cctacgaagc cggcatcgcc ctcggcagga ttcatcggcg tctccttaca
     4501 taaggctata gcgattgggc cgctacgcgc gccggcctcg c
//
LOCUS       DASUYC010000696         2642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1008101, whole genome shotgun sequence.
ACCESSION   DASUYC010000696 DASUYC010000000
VERSION     DASUYC010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008101"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..2581)
                     /locus_tag="VF276_11410"
     CDS             complement(119..2581)
                     /locus_tag="VF276_11410"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX6607510.1"
                     /translation="MAIKERPTAPGPATPGRRRRSLWKILLLVLGGIVLVVVLLAVGA
                     LTWLIRSPLPTTSGTVRIPGLSASVTVVRDKTGIPHITAANMADLFKAQGYVVAQDRL
                     WQMDFYRRVGAGRLSEVLGETTLDTDRFIRTVGWRRAAQKELDMLPDSFKAVLQDYAD
                     GVNAFIDTHPDSLPIEFTLLGYKPDPWTPLDSVTFGKVMAWDLSGNMDEEETLADLQA
                     KLGVARAAELLPDYPDGGTTIVQQSALPGNPLGRAPGPLRLSGVFGTDDTFGLIGSNN
                     WVVDGTRSASGHPLLANDPHLGVQNPSIWYAMQLHAADGSLDAEGVTFAGAPGIVVGH
                     NRDIVWGVTNLGPDTQDLFVETLDPADHPGQYQFQGQWLPLDILTETITIKGAPPEPL
                     VVRITRHGPLLNDVDSSLTKPTALQWTALQPGGLLPAVLNLDQATNWDEFHQALLTWD
                     VPGQNFVYADTQGNIGYQATGRWPIRKKGDGQVPVDGASGDYEWTGFVPYDEMPRVFN
                     PPDHYIVTANNRVVGPDYPYLIKARWGAWFRAERIAQMITAKPKLTMDDIKAIHYDTH
                     NVQAVEIAKYLAAAGGSDPATQQAAALFQNWDGNITADGPTAALYEITYGQVMTNTFA
                     DDLGGTLAAGYLRILRDESAILLNQLVADPTNAWWDDTRTPQHETRDDILRKSLQEAV
                     GILTAAQGGDRAQWSWGALHTITFAHTLGVAKPLDRLLNIGPYPMPGDAYTVATGGYA
                     ENKDSPHTYRQTNHPSMRMINDTGDWDQMQLIFAPGESGQVGSSHWDDQVVGWLHGQY
                     RTIPWSPAAVQQAADATLTLSP"
BASE COUNT          388 a          832 c          947 g          475 t
ORIGIN      
        1 ccacccgtcc gcgccccgca aaaagaggga gccaccccaa ccaccgggcg gctcccccac
       61 ctactcaatc cctgatcact cgtcgtactc agcactcagc tagggcactc agcactcgtc
      121 atggtgagag tgtgagcgtg gcgtcggcgg cttgctggac ggcggcggga gaccagggga
      181 tcgtccggta ctggccgtgg agccagccca ccacctggtc gtcccagtgg ctggagccga
      241 cctggcccga ctcgccgggc gcgaagatca gttgcatctg gtcccagtcg cccgtgtcgt
      301 tgatcatgcg catggacggg tggttggtct ggcgataggt gtgcggcgag tccttgtttt
      361 cggcgtagcc gcccgttgcc acagtgtagg cgtcgccggg catgggatag gggccgatat
      421 tgagcaggcg atccagcggc ttcgccacgc cgagcgtatg ggcgaacgtg atcgtgtgca
      481 gcgcgcccca ggaccattgc gcccggtcgc cgccctgcgc ggcggtcagg atgcccacgg
      541 cctcttgcag actcttgcgc aggatgtcgt cgcgtgtctc gtgctgtggg gtgcgggtgt
      601 cgtcccacca ggcgttggtc gggtccgcga ccagttggtt cagcaggatg gccgactcgt
      661 cgcgcaagat gcgcagatag ccggcggcta gcgtgccgcc caggtcgtcg gcaaaggtgt
      721 tggtcatgac ctgcccgtag gtgatctcgt agagcgcggc ggtggggccg tcggcggtga
      781 tgttgccgtc ccagttctgg aacagggccg cagcctgctg cgtggccggg tcgctgccgc
      841 ccgcggcggc caggtacttg gcgatctcga ccgcctgcac attgtgcgtg tcgtagtgga
      901 tcgccttgat gtcgtccatg gtgagcttgg gcttggccgt gatcatctgc gcgatacgct
      961 cggcacggaa ccaggcgccc cagcgggctt tgatcaggta gggatagtcc ggcccgacta
     1021 cccggttgtt ggcggtgacg atgtagtgat cgggcgggtt gaacacgcgc ggcatctcgt
     1081 cataggggac gaagccggtc cactcgtagt cgccgctggc cccgtccacg ggcacctggc
     1141 cgtcgccctt tttgcggatg ggccagcgcc cggtggcctg gtagccgatg ttgccctggg
     1201 tgtcggcata gacgaagttc tggccgggca cgtcccaggt aagcagcgcc tggtggaact
     1261 cgtcccagtt ggtcgcctgg tcgagattga gcacggcggg caacagccca cccggttgga
     1321 gggcggtcca ttgcagcgcg gtgggcttgg tcaggctgga gtccacatcg ttcagcagcg
     1381 gcccgtggcg cgtgatacgc acgaccagcg gctcgggcgg ggcacctttg atggtgatcg
     1441 tctcggtcag gatgtcgagc ggcagccact ggccctggaa ctggtactgg ccggggtgat
     1501 ccgccgggtc gagcgtttcc acgaagaggt cttgcgtatc ggggccgaga ttggtcacgc
     1561 cccagacgat atcgcggttg tggccgacca caatgccggg cgccccggcg aaggtcacac
     1621 cttcggcgtc gaggctgccg tcggcggcgt gcagttgcat ggcgtaccag atcgaggggt
     1681 tctgcacgcc caggtgcggg tcgttcgcca gcaggggatg gccgctggcg ctgcgcgtgc
     1741 cgtccaccac ccagttgttg ctgccgatca gcccgaaggt gtcgtcggtg ccgaacacgc
     1801 cgctgaggcg caacggcccc ggcgcccggc ccagcgggtt accgggcagc gccgactgct
     1861 ggacgatggt ggtgccgccg tcggggtagt cgggcagcag ttccgccgcc cgcgccacgc
     1921 cgagtttggc ctgcaagtcg gccagcgtct cctcctcgtc catgttgccg ctgagatccc
     1981 aggccatgac cttgccgaag gttacgctgt cgagcggcgt ccagggatcg ggcttgtagc
     2041 ccaggagggt gaattcgatg ggcaaggagt cggggtgggt gtcgatgaag gcgttgactc
     2101 cgtcggcata gtcctgcaag accgccttga agctgtcggg cagcatatcg agttccttct
     2161 gcgcggcccg gcgccagccg actgtgcgga tgaagcggtc ggtatcgagc gtggtttcgc
     2221 cgagcacctc cgacaggcgc cccgccccga cccggcgata aaagtccatc tgccagagcc
     2281 ggtcctgggc cacgacatag ccctgggcct tgaacaggtc ggccatgttg gcggcggtga
     2341 tatgggggat gcccgtcttg tcgcggacca cggtcacgga cgcggagaga ccggggatgc
     2401 gcacggtccc gctggtggtg ggcagggggc tgcggatgag ccaggtcagc gctcccacgg
     2461 ccagcagcac gaccaccagc acgatgccgc cgagcaccag cagcaatatt ttccaaagtg
     2521 agcggcggcg ccgtcccgga gtcgccggcc ccggcgcggt aggtcgttcc ttgatcgcca
     2581 tgcagtctcc ccctttggaa tagtcccccg attatagcac cgcgccgggg gctgtcaagc
     2641 cg
//
LOCUS       DASUYC010000697         4033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1309812, whole genome shotgun sequence.
ACCESSION   DASUYC010000697 DASUYC010000000
VERSION     DASUYC010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309812"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            465..770
                     /locus_tag="VF276_11415"
     CDS             465..770
                     /locus_tag="VF276_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607511.1"
                     /translation="MAKIWRHGDVLIGAVDKVPAGARPLSHVILARGEVTGHSHRIAE
                     RGAAHLYEVNGVLYLDVVAALATLIHEEHGPITLPQGRYRVWIQREYAPGEIRRVYD"
     gene            842..1840
                     /locus_tag="VF276_11420"
     CDS             842..1840
                     /locus_tag="VF276_11420"
                     /inference="COORDINATES: protein motif:HMM:NF042824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607512.1"
                     /translation="MQTLDAAQVATLIRRGKTPPPGPVRGPLHLKGLPIRHLPAGLQV
                     DMLDLTDCTALTALPADLRVRRLIVNGCTALTGLPAGLRCYELEARGTGLTGLPADLA
                     VAYRLDLSDSPALISLPPDLKTGVLILRNCPRLVALPEGLRVYFLDVAGCAALAHWPE
                     SAAVEIGCADLRGCRSLQALPPTVQNLAWVDLADSGITALPPGLAGAGLRWRGIAVEC
                     RHVFAPETITAAEVLRERNAERRRVILERMGYDAFLEQAHAEVLDSDRDAGGPRRLMR
                     VALADDEPLVCLAVRCPSTGRQYMLRVPPATRTARQAAAWIAGFDNPDDYAPLMET"
     gene            complement(1816..2694)
                     /locus_tag="VF276_11425"
     CDS             complement(1816..2694)
                     /locus_tag="VF276_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015348931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STM4011 family radical SAM protein"
                     /protein_id="HEX6607513.1"
                     /translation="MALDLTILYRGPLSSCNYGCRYCPFAKHREDHAAHAADARALDR
                     FVTWVAGRTADRLAILFTPWGEALIRRRYQQALVRLSHLPQVRRVAIQTNLSGRLDWL
                     EACNPARLALWATYHPTQTSRARFLAQCAELDRRGVRYSVGVVGVAEQAAEIAALRQA
                     LPPHVYLWINAYKAVPNYYTPDLLAAFTAVDPLFPINNQRHPSRGRPCRAGETAISVD
                     GDGTIRRCHFVRTPIGNLYDAGGVEAALRPRRCPNSTCSCHIGYIHLPHLELDAVFGD
                     GALERIPVDYVSMSGA"
     gene            complement(2685..4010)
                     /locus_tag="VF276_11430"
     CDS             complement(2685..4010)
                     /locus_tag="VF276_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014396978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STM4012 family radical SAM protein"
                     /protein_id="HEX6607514.1"
                     /translation="MIPTLSARPTTPYAAYTYAYPHKTAYRPLAAPVPLADLWAGEAR
                     DALSLYIHLPFCEMRCGFCNLFTTANPQADFVTAYLDALMRQARQVRAALGTARIARF
                     ALGGGTPTYLTAGELAQVFDLAADLYGVTPALVPTSVETSPGTATPERLAVLRERGVD
                     RVSIGVQSFLEAEARAAGRAQRTAEVHAALAAIRRAQIPTLNIDLIYGLPGQTVATWR
                     TSVQAALAYAPEEIYLYPLYTRPLTGLGRRGTPEAENDVRLACYRAGRDLLLAAGYRQ
                     VSLRMFRAGDAPEAGGPAYCCQEDGMVGLGCGARSYTRALHYAAEYAVGAAGIRAILA
                     AYVAQPDAAFAVAPYGCPLDEDEQRRRYLIQSLLQVEGLERAAYRRRFGTDARADWPV
                     LEEWAAWGWLEANATWVRLTPAGLEYSDWIGPALTSPAVRARMESYAWR"
BASE COUNT          567 a         1416 c         1488 g          562 t
ORIGIN      
        1 cgccgcctgg gtggccgacg cgggcgattt cgacctgctc atcggcacca gcagcgtaga
       61 cctgccacac acggcccgga tccggctggc gcgcgagtgg accgagcccg cgtgatcccg
      121 gccaccggcg cgggccggtg cacatccaaa tagaccacgc gcggcgtgga atccgtccgc
      181 gaccggcagc aggtgaagct gccggtcgcg tgggttacgg ccccgcggtg cccacactcg
      241 actccagcaa atttccctga gtgggcgtca aagacctcgg cggcgccatg gctgatgatg
      301 ggctcgggtc gtccaggagt gcgcccatcc ttgggccacg cgaacagcgg gccgcgcggc
      361 gcccggttcc ggtttccgcc cgcacgcggc gatatatata gtgtgcgggc gcggggccgt
      421 gcctgcaccg ggggcgcaaa cccggcgtga aggaggatcg ggcgatggcg aagatctggc
      481 gacatggcga tgtgttgatc ggggcggtgg acaaggtgcc cgcgggcgcg cgcccgctgt
      541 cccatgtgat cctggcgcgc ggggaggtta cgggccacag ccaccgcatc gcggagcgcg
      601 gggccgccca cctgtacgag gtcaacgggg tgctctatct cgacgtggtg gccgcattgg
      661 cgacgttgat ccacgaggaa cacggaccga tcaccttgcc ccagggccgg taccgcgtct
      721 ggatccagcg cgagtacgcg ccgggcgaga tccgccgcgt gtacgattga ggcgcctgtt
      781 tgtccgccgg cgcccatcgc tgctcacaac acgcgggcgc ctagccccag gggatcgacc
      841 gatgcaaacg ctggacgccg ctcaggtcgc gacgctgatt cgccgcggca agaccccgcc
      901 gccggggccg gtgcgcgggc cgctgcacct caagggcctg ccgatccgcc acctgcccgc
      961 cggcctccag gtcgatatgc tcgacctgac cgattgcacc gcgctgacgg cgctgccggc
     1021 ggatctgcgg gtgcggcgcc tgatcgtgaa cggctgcacg gcgctgaccg ggctgcccgc
     1081 cgggctgcgc tgctatgaac tcgaagcccg cggcaccggg ctgactgggc tgcccgccga
     1141 tctggcggtg gcctaccggc tggatctgag cgacagcccc gccctgatat cgctgccgcc
     1201 ggatctaaaa accggcgtgc tcatcttgcg caactgcccc cgattggttg cgctgcccga
     1261 gggcctgcgc gtctacttcc tggatgttgc cgggtgtgcc gcgctggcgc actggccgga
     1321 gtcggcggcg gtggagatcg gctgcgccga cctgcgcggc tgccgcagcc tgcaggcgct
     1381 gccgcccacg gtgcagaacc tggcctgggt cgatctggcc gatagtggga taacggccct
     1441 gccgccgggg ctggccggcg ccgggctgcg ctggaggggc atcgccgtcg agtgccgcca
     1501 cgtcttcgcg ccggagacga tcaccgccgc cgaggtgttg cgggagcgca acgcggagcg
     1561 gcggcgcgtc atcctggaac gcatgggcta tgacgccttt ctggagcagg cgcacgccga
     1621 ggtgctcgat agcgaccgcg acgccggtgg cccgcgccgg ctcatgcgcg tggcgctggc
     1681 ggacgatgag ccgctcgtct gcctggccgt gcgctgccca tcgaccgggc ggcaatatat
     1741 gttgcgcgtg ccgcccgcga cgcgcaccgc ccgccaggcc gccgcctgga tcgccggctt
     1801 cgacaacccc gacgactacg cgccgctcat ggagacataa tcgaccggga tgcgctccag
     1861 cgccccgtcg ccgaacacgg cgtccagctc caggtgcggc aggtggatat agccgatgtg
     1921 gcagctacag gtgctgttgg ggcagcggcg cgggcgcagg gccgcctcga ccccgcccgc
     1981 gtcgtagagg ttgccgatgg gcgtgcgcac gaaatggcag cggcggatcg tgccgtcgcc
     2041 gtccacgctg atcgccgtct cgcccgcccg gcagggccgg ccccggctgg ggtggcgctg
     2101 gttgttgatc gggaacagcg ggtcgacggc ggtaaaggcg gccagcaagt ccggggtgta
     2161 gtagttgggc acggctttgt aggcgttgat ccagaggtag acgtgcggcg gcagcgcctg
     2221 ccgcagggcc gcgatctccg ccgcctgctc ggccacgccc accacgccca cgctgtagcg
     2281 cacgccccgg cgatccaact ccgcgcattg ggccaggaag cgggcacggg aggtctgcgt
     2341 cgggtggtag gtggcccaga gcgccagccg cgccgggttg cacgcctcca gccagtcgag
     2401 ccggcccgac agattggtct ggatcgccac gcggcgcacc tgcggcaggt gcgagagccg
     2461 gaccagcgcc tgctggtagc gccgccggat cagcgcctcg ccccacgggg tgaagaggat
     2521 ggcgagccgg tccgccgtgc gcccggccac ccaggtgaca aaccggtcga gggcgcgggc
     2581 gtcggcggcg tgggctgcgt gatcctcgcg atgcttggcg aacgggcagt agcggcagcc
     2641 gtagttgcaa ctgctgagcg gcccgcggta caggatggtc aggtctagcg ccatgcgtag
     2701 ctctccatgc gcgcccgcac cgcgggggag gtgagcgcgg gaccgatcca gtcggagtat
     2761 tccaggcccg ccggggtcag ccgcacccat gtcgcgttcg cctccagcca accccaggcc
     2821 gcccactcct ccagcaccgg ccagtccgcc ctcgcgtccg tgccgaagcg ccggcggtac
     2881 gccgcccgtt ccaggccctc gacctgcaag agcgattgga tcaggtagcg gcggcgctgt
     2941 tcgtcctcgt ccagcgggca cccatacggc gcgacggcga acgccgcgtc gggctgggcg
     3001 acgtaggcgg ccaggatggc ccggataccg gcggccccga ccgcgtactc ggcggcatag
     3061 tgcagcgccc gcgtgtagga tcgcgccccg cagcccaggc cgaccatgcc gtcttcctgg
     3121 cagcaatagg ccgggccgcc cgcctccggc gcgtccccgg cccgaaacat gcgcagcgac
     3181 acctggcgat agcccgctgc cagcagcaga tcgcgccccg cccggtagca ggccaggcgc
     3241 acatcgttct ccgcttccgg cgtgccgcgc cgccccagcc cggtcagcgg gcgcgtgtag
     3301 agcgggtaca ggtagatctc ttcgggcgcg taggccagcg cggcctgcac cgacgtgcgc
     3361 caggtggcga ccgtctgccc cggcaggcca tagatcaggt cgatattgag tgtcgggatc
     3421 tgcgcccggc ggatcgcggc gagcgcggca tggacctcgg cggtgcgctg ggcgcggccc
     3481 gcggcgcgcg cttcggcttc caggaagctc tgcacgccga tgctcacccg gtccaccccg
     3541 cgctcgcgca gcacggccag ccgctcgggt gtcgccgtgc cgggcgaggt ttccaccgac
     3601 gtgggcacca gggccggggt caccccgtag agatcggcgg ccaggtcgaa cacctgggcc
     3661 agttcgcccg ccgtcagata ggttggcgtg cccccgccga gcgcgaagcg cgcgatgcgg
     3721 gccgtgccca gcgccgcgcg cacctgccgc gcctggcgca tgagcgcatc caggtaggcg
     3781 gtcacgaaat cggcctgcgg gttggcggtg gtgaacaggt tgcagaagcc gcagcgcatc
     3841 tcgcaaaacg gcagatggat atagagcgag agggcgtcgc gcgcttctcc ggcccacaga
     3901 tcggccagcg gcaccggcgc cgccaggggc cggtaggccg tcttgtgcgg gtaggcatag
     3961 gtgtaggcgg catagggcgt cgtggggcgg gcggacaggg tcggaatcat agcggctcct
     4021 gggcgaggac ggt
//
LOCUS       DASUYC010000698         5960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_567959,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000698 DASUYC010000000
VERSION     DASUYC010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5960
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567959"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="VF276_11435"
     CDS             <1..971
                     /locus_tag="VF276_11435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6607515.1"
                     /translation="GLVAADLKQALGIPFVITFHALGRVRRLYQREADQFPVARLAIE
                     ERLVAEADRVVAECPQDEEDLIRLYHGDPARITIVPCGFDPAECAPVGRARARAVLGL
                     PPGEPILLQLGRLVPRKGVDNVIRSLAYLRDRHGIVARLLVVGGDSDVPDPVITPELA
                     RLQGIAQEAGVAEQVLFVGRRGRDALKYYYSAADIFLTTPWYEPFGITPLEAMACGTP
                     VIGANVGGIAYTVQDGATGYLVPPKDPAALAERIAYLYAHPHLRAAFGRRAIRRVNEL
                     FTWDKVAQGMDALYQDVLDAAPVAAALQQAIRAVRPRRRRGEMPAA"
     gene            1008..1751
                     /locus_tag="VF276_11440"
     CDS             1008..1751
                     /locus_tag="VF276_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6607516.1"
                     /translation="MQTSQTSQILRGKVALVTGGGRGLGAAICQSLGEAGAIIVAADM
                     RTELVTQAVQGLQAQDIQATALHLDVTDARQAEAAVQETIRKCGRLDILINNAGTDMT
                     APVEELTLDDWDRVLAVNLRGPFVMAKLAFPLMRRQGHGHIVNIVSTAAKRAWAEASA
                     YHASKWGLLGLSHALHVEGRAHHIKVTAVVAGGMRTPFLLDRFPDLDPGLLQDPRNVA
                     DTIRFVLTQPDETVIPEVTVIPMRETSWP"
     gene            1754..2377
                     /locus_tag="VF276_11445"
     CDS             1754..2377
                     /locus_tag="VF276_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015190300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX6607517.1"
                     /translation="MPMRAVFLDKDGTLVEDVPYNVDPRQIRLAAGSAAGVRLLHAAG
                     YRLFVISNQSGVARGLFPESALAGVEAALRRLLADAGAPLSGFYYCPHYPDGVVPAYS
                     VACDCRKPAPGLIRRAAADHDLDLPASWFIGDILDDVEAGHRAGCRAILLDNGHETEW
                     LPGPGRAPDYMAPDLAAAARTILATDRRAGMVSRDGRAGPAPVGGPL"
     gene            2374..3954
                     /gene="rfaE2"
                     /locus_tag="VF276_11450"
     CDS             2374..3954
                     /gene="rfaE2"
                     /locus_tag="VF276_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016875262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HEX6607518.1"
                     /translation="MNAHLLNVIDACAGRRVLVIGEAMLDSYLEGAAGRLCREAPVPV
                     VNVTCRTDVPGGAANTAANVAALGAHVVFLSAIGADAEGELLRRALEARAVPVTQVLT
                     SPARHTPVKHRVVAAGQILVRFDQGSTDPVDAPTEQALIARLGYWFPRCDAVIVSDYG
                     YGILTPRIIAALADLQARLPRVLVVDARDLAAYRQVGSTAVKPNYEETVQLLGLKAGR
                     DSRVAEVTAQGARVLDLAGTRIAAITLDTDGGLIFERGQAPYRIYARPGPHSRATGAG
                     DTFVSTLALALAAGAPTPIAAELAAAATAVVVARDGTVCCSAAELRESLSAGGRYVSS
                     LARLTALVETYRAQSRRIVFTNGCFDILHRGHSSFLSQAKALGDVLIVGINSDAGVRR
                     LKGPGRPINPLEDRVQVLAALSGVDHVIAFDEAMPDALIRAVRPDIFAKGGNYTREDL
                     PEAALVEALGGVVRILPYLQDHSTAHIIRRTRAVHALPAFTENGLADGNAVRDVSRAP
                     YSLPLGDGVYDAPETGAA"
     gene            3951..5057
                     /gene="waaF"
                     /locus_tag="VF276_11455"
     CDS             3951..5057
                     /gene="waaF"
                     /locus_tag="VF276_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015154245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEX6607519.1"
                     /translation="MNPRSAWRRARNVLCIRLDTIGDVLMTTPALRAVQESAPGRRVT
                     LLTSPAGAAVAGLVPEIDACLVYDAPWMKATAPRPDSAPEYAMAERLRAGGFDAAIIF
                     TVYSQNPLPAAFLCYLAGIPLRLAHCHENPYQLLTDWVPDPEPGGGVRHEVRRQLDLV
                     ATVGYHTTDERLSLRVPAGAELRVLRTLNEAGVDLARPWIVIHPGATAPSRRYPPEQF
                     AVVARRLVAERGYQVLLTGMAGEQELIAGIQAAAGVPLFALVDRFDLGELAALLARTP
                     LLIANNTGPVHMAAALGTPVVDLYALTNPQHTPWAVPHRVLNHDVPCKYCYKSVCPAG
                     HHNCLRLVTPDMVLAAAGELLTATAAPAPVGGSL"
     gene            5054..5902
                     /locus_tag="VF276_11460"
     CDS             5054..5902
                     /locus_tag="VF276_11460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6607520.1"
                     /translation="MTAAVDILIPTYARPAALAVTLTSLCAQTFRDFRVVISDQTEDG
                     PGAESGEVRAAIRVLSAHGQPVEVHRHLPRRGMAEQRQFLLDQATAPHALFLDDDLIL
                     EPYVAGQMHDALVREGCGFVGCAVIGLSFIPDVRPHEQAIEFWDGPVRPEVVQPDTPE
                     WNRYRLHNAANPYHVQQRLGLAPERARTYHVAWVGGCVMYDTAKLRAVGGFSFWRELP
                     PTHCGEDVLAQWRVMARYGGCGLLPSGVYHQELPTTLPDRTVNAPRVLGVTPAPAGDP
                     ASAAIH"
BASE COUNT          826 a         2184 c         2110 g          840 t
ORIGIN      
        1 cggggctggt cgccgccgat ctcaagcagg cgctgggcat cccgttcgtg atcacgttcc
       61 atgcgctggg acgggtgcgg cggctgtacc agcgcgaggc cgaccagttc ccggtggcgc
      121 ggctggcgat cgaagagcgc ctggtggccg aggccgaccg ggtggtggcc gagtgcccgc
      181 aggacgagga agacctgatc cgcctctacc atggcgaccc ggcgcggatc accatcgtgc
      241 cctgcggctt cgacccggcg gagtgtgcgc cggtgggccg ggcgcgggcc cgcgccgtgc
      301 tgggcctgcc gcccggcgag ccgatcctgt tgcagttggg gcggctggtg ccgcgcaagg
      361 gcgtggacaa cgtgatccgc agcctggcct atctgcgcga ccggcacggc atcgtggccc
      421 gcctgctcgt cgtcggcggc gactccgacg tgcccgaccc ggtgatcacg ccggaactcg
      481 cgcggctcca gggcatcgcc caggaggccg gggtggccga gcaggtactg ttcgtgggcc
      541 gccggggccg cgacgcgctc aaatactact acagcgccgc cgacatcttc ctgacgacgc
      601 cctggtacga gcctttcggc atcacgccgc tggaggccat ggcctgcggc accccggtga
      661 tcggcgccaa cgtcggcggc atcgcataca cggtgcagga cggcgcgacc ggctacctcg
      721 tgccgccaaa ggacccggca gccctggccg agcgcatcgc ctacctgtat gcgcacccgc
      781 acctgcgcgc ggcgtttggc cgccgcgcca tccggcgggt caacgaactg ttcacctggg
      841 acaaggtcgc gcagggcatg gacgcgctct accaggatgt gctcgatgcg gcccccgtcg
      901 ccgcagcact ccagcaggcg atccgcgctg tgcggccacg ccggcgccgg ggcgagatgc
      961 cggcggccta gaccgcaccg gagacaccga tggaaggacc ctcgaccatg caaacatcgc
     1021 aaacatcgca aatactccgc ggcaaagtgg ccctcgtcac cgggggcggg cgcggcctgg
     1081 gcgcggcgat ctgccagtcg ctgggcgagg cgggcgcgat catcgtggcc gccgacatgc
     1141 gcaccgaact ggtgacgcag gccgtccagg ggctgcaggc gcaggacatc caggccaccg
     1201 cgctgcacct cgacgtgacc gacgcgcggc aggccgaagc cgccgtgcag gagacgatcc
     1261 gcaagtgcgg gcggctcgac atcctgatca acaacgcggg caccgacatg accgcgccgg
     1321 tggaggagtt gacgctggac gactgggacc gcgtgctggc cgtgaacctg cgcggcccct
     1381 tcgtcatggc caagctggcc ttcccgctga tgcgccgtca gggccacggg catatcgtca
     1441 acatcgtctc caccgcggcc aagcgggcat gggccgaggc ctcggcctac cacgccagca
     1501 agtgggggct gctggggctg agccacgcgc tgcacgtgga gggccgcgcc caccacatca
     1561 aggtcacggc ggtggtcgcg ggcggcatgc gcacgccgtt cctgctggac cgcttcccag
     1621 acctcgatcc ggggctgttg caggacccgc gcaacgtcgc cgacaccatc cgcttcgtgc
     1681 tgacccagcc cgacgagacg gtgatcccgg aggtgacggt catcccgatg cgcgagacct
     1741 cgtggccgta ggaatgccca tgcgcgcagt ctttctcgac aaagacggca ccctggttga
     1801 ggacgtgccg tacaacgtgg acccgcggca gatccgcctg gcggcgggct ccgccgccgg
     1861 tgtgcggctg ctgcacgcgg cgggctaccg gctgttcgtc atctcgaacc agtcgggtgt
     1921 ggcgcggggc ctcttcccgg agagcgcgct ggccggggtg gaggcggcgc tgcgccggct
     1981 gctggcggac gcgggcgcgc ccctgagcgg cttctactac tgcccgcatt accccgacgg
     2041 tgtcgtgccc gcctacagcg tggcctgcga ctgccgcaag ccggcgcccg gcctaatccg
     2101 ccgggcggcg gcggaccatg atctcgatct gcccgcctcg tggttcatcg gcgacatcct
     2161 ggacgacgtg gaggcggggc accgggcggg ctgccgggcg atcttgctcg acaacgggca
     2221 cgaaaccgag tggctgcccg gccccggtcg cgcgcccgac tacatggcgc cggatctggc
     2281 ggcggcagcg cgcacgatcc tggcgaccga ccgccgggcc ggcatggtat cgcgggatgg
     2341 gcgcgccggc cccgcgcccg tcggaggacc cctgtgaacg cccatctgct caacgtcatc
     2401 gacgcgtgcg ccgggcgccg ggtgctggtc atcggcgagg cgatgctcga cagttacctg
     2461 gaaggcgcgg cgggccggct gtgccgcgag gcgcccgtgc cggtggtcaa cgtgacctgc
     2521 cgcaccgatg tgcccggcgg cgccgccaac accgccgcca atgtcgccgc cctcggcgcg
     2581 cacgtcgtct tcctgtcggc catcggagcc gacgctgagg gtgaattgct gcgccgggcg
     2641 ctggaagcgc gcgcggtgcc ggtcacgcag gtgctgacca gcccggcccg gcacaccccg
     2701 gttaaacacc gcgtggtcgc cgccgggcag atcctggtgc gcttcgacca gggcagcacc
     2761 gacccggtcg atgccccgac cgagcaggcg ctgatcgccc gcctgggcta ctggttcccg
     2821 cgctgcgacg cggtgatcgt ctcagactac ggttacggca tcctgacccc gcgcatcatc
     2881 gcggccctgg ccgatttgca ggcccgcctg ccgcgcgtgc tggtggtcga tgcccgcgac
     2941 ctggcggcgt atcgccaggt cggtagcacc gccgtcaagc ccaactacga ggagacggtg
     3001 cagttgctcg gcctgaaggc gggccgcgac tcgcgcgtgg ccgaggtcac ggcgcagggg
     3061 gcgcgggtgc tggacctggc gggcacgcgc atcgccgcga tcaccctgga taccgatggc
     3121 ggcctgatct tcgagcgcgg gcaggcgccc taccgcatct acgcgcggcc cggcccgcac
     3181 tcccgcgcca ccggggcggg cgacacgttc gtgagtaccc tggccctggc gctggccgcg
     3241 ggcgccccca cgcctatcgc cgccgaattg gccgccgccg cgaccgcggt cgtcgtggcg
     3301 cgggacggca cggtctgctg ctcggcggcg gaactgcgcg agtcgctgag cgccgggggc
     3361 cgctatgtgt ccagcctcgc ccgcctcacc gcgctggtgg agacctatcg cgcgcagagc
     3421 cggcgcatcg tgttcaccaa cggctgcttc gacatcctgc accgcggcca cagcagcttc
     3481 ctgagccagg ccaaggcgct gggcgacgtg ctgatcgtcg gcatcaactc ggacgccggg
     3541 gtgcgccgcc tgaaggggcc aggccggccc atcaacccgc tcgaagatcg ggtgcaggtg
     3601 ctggccgccc tcagcggcgt ggaccacgtg atcgccttcg acgaggcgat gccggacgcg
     3661 ttgatccgcg cggtgcgccc ggacatcttc gccaagggcg gtaactacac ccgcgaggac
     3721 ctacccgaag cggccctggt cgaagcgttg ggcggggtcg tgcgcatctt gccctatctg
     3781 caagatcact cgacggcgca catcatccgc cgcacgcggg ccgtccacgc gctgcccgcc
     3841 ttcaccgaaa acggtctcgc cgacgggaac gccgtccgcg atgttagccg cgcgccgtac
     3901 agcctgccgc ttggcgatgg cgtgtacgac gcgccggaga cgggggccgc atgaaccccc
     3961 gcagcgcgtg gcgccgggcc cgcaacgtcc tgtgcatccg cctcgatacc atcggcgatg
     4021 tgctcatgac gactccggcc ttgcgcgccg tgcaggagtc cgcgcccggt cgccgcgtca
     4081 cgctgcttac gtccccggcg ggagccgccg tggcggggct ggtgcccgaa atcgacgcct
     4141 gcctggtcta cgacgcgccc tggatgaagg ccaccgcgcc ccgccccgac agcgcccccg
     4201 agtacgcgat ggccgagcgc ctgcgcgcgg gcgggttcga cgccgcgatc atcttcaccg
     4261 tttacagcca gaatccgctg ccggcggcct tcctgtgcta cctggccggt atcccgctac
     4321 ggctggccca ctgccacgag aacccttacc agttgctcac cgactgggtg cccgacccgg
     4381 agccgggggg cggcgtccgc cacgaggtcc gccgccaact ggacctggtc gccaccgtgg
     4441 gctatcacac gacagatgag cggctctcgc tgcgcgtccc ggcgggcgcc gagttgcgcg
     4501 tgttgcgcac gctgaacgag gcgggcgtgg acctggcccg gccctggatc gtgatccacc
     4561 ccggtgcgac ggcgccgtcg cggcgctatc ccccggagca gttcgccgtg gtcgcccgcc
     4621 gcctggtggc cgagcggggc taccaggtgc tgctgaccgg catggcgggc gagcaggagt
     4681 tgatcgccgg catccaggcg gcggcgggcg tgccgttatt cgccctggtg gatcgcttcg
     4741 acctgggcga actggcggcg ctgctggcgc gcacgccgct gctcatcgcc aacaacaccg
     4801 gccccgtgca catggcggcg gccctgggca cgcccgtcgt ggacctgtac gcgctgacca
     4861 acccgcagca cacgccctgg gccgtgccgc accgcgtgct caaccacgac gtgccctgca
     4921 aatactgcta caagagcgtc tgcccggcgg gccaccacaa ctgcctgcgc ctggtgacgc
     4981 ccgacatggt gctggcggcg gcgggggagt tgctgaccgc gaccgccgcc ccggcccccg
     5041 tgggaggtag cctatgactg ccgccgtcga cattctgatc ccgacctacg cgcgccccgc
     5101 tgccctggcc gtcacgctga cgagcctctg cgcgcaaacc ttccgcgatt tccgcgtcgt
     5161 catctccgac cagacggagg acgggccggg cgcggagagc ggcgaagtgc gggccgccat
     5221 ccgcgtgctg agcgcgcacg gccagccggt cgaggtccac cgccacctgc cccggcgcgg
     5281 catggccgag caacgccagt tcctgctcga ccaggccacg gcgccccatg cgctcttttt
     5341 agacgacgac ctgatcctcg aaccgtacgt cgccgggcag atgcacgacg cgctggtgcg
     5401 cgagggctgc ggcttcgtcg gctgcgccgt catcggcttg agcttcatcc ccgatgtgcg
     5461 cccgcacgag caggccatcg agttctggga cggcccggtg cggcccgagg tcgtgcagcc
     5521 cgatacgccg gagtggaacc gctaccggct gcacaacgcg gccaacccct accacgtgca
     5581 gcagcggctg gggctggcgc cggagcgggc gcggacttac cacgtggcct gggtgggcgg
     5641 ctgcgtgatg tacgacaccg ccaaactgcg cgcggtgggc ggcttcagct tctggcgcga
     5701 actgccgccc acgcattgcg gcgaggacgt gctggcccag tggcgggtca tggcccgcta
     5761 cggcggctgc ggcctgctgc cgtccggcgt ctaccaccag gagttgccca ccacgctgcc
     5821 cgaccggacg gtgaacgccc cgcgcgtgct gggcgtgact cccgctccgg cgggcgaccc
     5881 cgcgtccgcg gcgatacatt gagcaggcaa tccaccgaga cgtgtcattc tgagcgcagc
     5941 gaagaatctc cggctcgttg
//
LOCUS       DASUYC010000699         3729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1261235, whole genome shotgun sequence.
ACCESSION   DASUYC010000699 DASUYC010000000
VERSION     DASUYC010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261235"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..155)
                     /locus_tag="VF276_11465"
     CDS             complement(<1..155)
                     /locus_tag="VF276_11465"
                     /inference="COORDINATES: protein motif:HMM:NF019139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HEX6607521.1"
                     /translation="MNLAELESKTIDELQDLAKELDISGYSRLKKQELAFRILQAQTE
                     QQGNIFAA"
     gene            complement(167..1186)
                     /gene="glpX"
                     /locus_tag="VF276_11470"
     CDS             complement(167..1186)
                     /gene="glpX"
                     /locus_tag="VF276_11470"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEX6607522.1"
                     /translation="MAEEITRNLGMELVRATEAAAMASARWMGRGDKNAADQAAVNAM
                     RYALGNVDMHGVVVIGEGEKDEAPMLFIGEEIGNGHAPEVDIAVDPIDGTRLTALGLP
                     GAIAVVAAAPRGTLYGAPPGIFYMEKIAVGPEAVGAIDITKPIVENVRSVAGLKGCDP
                     SDITVVILDRPRHDYIVKAVREVGARIKLIGDGDVAGAIAAASPNTGVDMLIGIGGAP
                     EAVIAATAIRCIGGEMQCRLYPRNERERWLVEGAGVDISQVLTLADLVASDDVYFAAT
                     GITDGDLLNGVHYYSNGATTHSIIMRGKTGTIRRMEARHRLKKLALVSRLAYTASVEP
                     QSIPS"
     gene            complement(1655..1749)
                     /locus_tag="VF276_11475"
     tRNA            complement(1655..1749)
                     /locus_tag="VF276_11475"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1713..1715),aa:Ser,seq:gct)
     gene            complement(1927..>3729)
                     /locus_tag="VF276_11480"
     CDS             complement(1927..>3729)
                     /locus_tag="VF276_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607523.1"
                     /translation="LLAGHMLLAGVGVLALGRVALRLSPLAALVAALAFMATPRLIGH
                     LGAGHVTIVQTVAWFPWVAVLCWATVRDPARWAVPLGGALALMGLAGHPQMAYYGGLL
                     AAGLSLALLFVRGRCAGGRALAASVAGLAAAGLLAGLLAAVHLVPLLEFTAHSTREQT
                     VRSADALDLLPFLGALVGFRIPSPVPHEAIFDPGLGILALAGVGVAARRRAGVPIVLG
                     VALGAGLALGVSSPVYRLAAAVLPSFDLFRGLARIWFVGLLGIALLAGLGADAVIAAI
                     RRSGERILVAAGAAAGILLAISLVVADQGLAHVDAVQPRVEPSALERRAAEVAGADRI
                     YGVQRNVRQLAAVELHVDLADGWDPLLIQPYVAFMQRAGGYSFPGYELAVPPFEVYDP
                     GYPTSRAAQPDARLLGLFDIGTVLSRVPLTDTRLVQIDQVDGTLIYRNRANAGPAYLV
                     APGAGGTLPAFADLQPLAATVRVAARQAEQWSGTIDSPGGGILIIGTPAFPGWVARLD
                     GQPVPLLAIDGVLPALRMPPGPHQWVYSYAPASVRLGAGLSLAGLVAALVWLIGFALV
                     SRRRAATGLPRRATPLRGGWGLAPQWRGVRRSGR"
BASE COUNT          644 a         1268 c         1255 g          562 t
ORIGIN      
        1 gcggcaaaga tattgccctg ctgctcggtc tgggcctgaa ggatacggaa ggccagctct
       61 tgctttttca gccggctata gcccgaaata tcgagctcct tcgccaggtc ttgtaattcg
      121 tcgattgttt tactttctaa ctctgccagg ttcatggtgg tcctctttat gaaggtatag
      181 attggggttc tactgacgca gtgtaggcca gtcgggaaac aagggctaat ttcttcaggc
      241 gatggcgggc ttccatccgg cggatggtgc cggttttgcc gcgcatgatg atggaatggg
      301 tggtggcacc gttgctgtaa taatgcacgc cgttgagcaa atccccatcg gtgataccgg
      361 tcgcggcgaa gtagacatcg tcggacgcga ccaggtcggc cagggtcaag acctggctga
      421 tgtcgactcc ggcgccctcg accagccagc gttcgcgttc gttgcgcgga tagagccggc
      481 actgcatttc gccgccgatg cagcggatgg ccgtggcggc aatgacggcc tccggcgcgc
      541 cgccgatgcc gatgagcata tcgacgccgg tgttggggct ggccgccgcg atagcacctg
      601 ccacatcgcc gtcgccgatg agcttgatgc gcgcgcccac ctcccgcacg gctttgacga
      661 tatagtcatg ccgcgggcgg tccaggatca ccaccgtgat gtcgctcggg tcacagcctt
      721 taaggccggc cacgctgcgt acgttctcca cgatgggttt tgtgatgtcg atggccccca
      781 ccgcttcggg gccgaccgcg attttctcca tataaaagat accgggaggc gcgccgtaga
      841 gcgtgccgcg cggggccgcc gccacgaccg cgatggcgcc gggcaagccg agcgccgtca
      901 gccgcgtccc gtcgatggga tcgacggcga tatcgacctc cggcgcgtgc ccgttgccga
      961 tctcctcgcc gatgaacagc atcggcgcct cgtccttttc gccttcgccg atgacgacca
     1021 cgccgtgcat atcgacattg cccagcgcgt agcgcatggc gttcacggcc gcctgatcgg
     1081 ccgcgttctt gtcgccgcgg cccatccagc gggccgaggc catggccgcg gcctcggtgg
     1141 cgcggaccag ttccataccg agattccggg taatttcctc cgccatacga tgctcactcc
     1201 tttgtgatca tgcgtgatcc atacccgcgc aataattcgg tcatacgcaa gtgttcgagc
     1261 aacaagggat caactctaca ggtgggtact cagttcggtt gggccatcat tcggcgccgc
     1321 aagcaggacc gcgcctcgac atgatgcaca ggactcccgg ctaacggcgc ataacatagg
     1381 agtgctgtgc gacgcgaggg ccgcttgtac gcatggaaga tcgtccagga gttgccgcac
     1441 ggtttacacc ggcggcgcta tcggtacagc gcaagatatg tgccaagact agtggcgcgc
     1501 ccggctcatt agctgagcac tggccccctg tcgactccgc gtcacgcatg tcctactcgt
     1561 gatatacctg ctgctacggc cgtaggataa acaagggagg gaaacggccg gttcagcccg
     1621 gcaacgttca gcgacaggtc ccaggtacag gggttggcgg ggagataggg attcgaaccc
     1681 tagaagcagt tttaaacacc gcttaaccgc ttagcaggcg gccccattcg accactctgg
     1741 catctcccca ggtcgtgtgg acttgcttat tataacatag ccgcggatcg attgcaagct
     1801 acctcacggg ccattttcgg gcggttggcg ttgtcggcaa atcggtttgt cctcagcggt
     1861 attttcgccg ctactctcag ccggcgccgg ccggtgggcc ggtgccttca cgatccatat
     1921 acgcaatcag cggccggatc gtcgcacgcc tcgccattgc ggcgccagac cccatccccc
     1981 gcgtaggggc gtcgcgcggc ggggcaggcc ggtcgcggcg cggcgacggg atacgagggc
     2041 gaagccaatc agccagacca gcgcggcgac cagccccgcc agcgagaggc ccgcgcccag
     2101 ccgcacactg gcgggcgcgt agctatagac ccactggtgc gggccggggg gcatgcgcag
     2161 cgcgggcaat acgccgtcga tcgccaggag gggcaccggt tggccgtcga gccgggcaac
     2221 ccagccggga aaggcgggcg tgccgatgat caggatgccg ccgccgggcg agtcgatggt
     2281 gcccgaccat tgttcggcct gccgcgcggc gacgcgcacg gtcgcggcga gcggttgcag
     2341 atcggcgaag gcgggtagcg tgccgccggc gccgggggcg accagatagg ccggccccgc
     2401 gttggcccgg ttgcgataga tcaaggtgcc gtctacctgg tcgatctgga cgaggcgggt
     2461 gtcggtgagg ggcacgcgcg agagcacggt gccgatgtcg aacaggccca gcagccgggc
     2521 atcgggctgg gcagcgcgag aggtgggata gccgggatcg tagacctcga agggcggcac
     2581 cgcgagctcg tagccgggga agctgtaccc cccggcccgt tgcatgaacg cgacataggg
     2641 ttggatcagc agtgggtccc agccgtcggc cagatcgacg tgtaactcga cggcggccag
     2701 ttgccgcacg ttgcgctgga cgccgtagat gcggtcggcc ccggcgacct ccgccgcgcg
     2761 ccgctccaga gccgacggct ccacgcgcgg ctgcaccgca tcgacatggg ccagcccctg
     2821 gtcggccacg accaggctga tcgcgagcag gatgcccgcc gccgcgcccg ccgcgaccag
     2881 gatgcgctcg ccgctacggc ggatggcggc gatgacggcg tccgcgccga gcccggccag
     2941 cagggcgata cccagcagcc ccacgaacca gatgcgcgcc agtccgcgga acaggtcgaa
     3001 gctgggcagc accgccgccg ccagccggta gacgggcgag gacacgccca gcgccagccc
     3061 ggcgcccagc gccacgccca gcacgatcgg gacgccggcc cggcgccgcg ccgccacccc
     3121 cacgcccgcc agggccagga tgccgaggcc gggatcgaag atcgcctcat gcggcaccgg
     3181 cgaggggatg cggaagccca ccagggcgcc caggaagggc agcaggtcca gcgcgtcggc
     3241 gctgcgcacc gtctgctcgc gggtggagtg ggcagtgaac tccagcagcg gcacgagatg
     3301 taccgccgcc agcaaccccg ccagcagccc ggcagccgcc aaccccgcga ccgatgccgc
     3361 cagcgcccgg cccccggcgc agcgcccacg cacgaacagc agcgccagcg agagacctgc
     3421 ggccagcaac ccgccgtagt aggccatctg cggatgcccg gccagcccca tcagcgccag
     3481 ggcgccgccc agcggcaccg cccagcgtgc ggggtcgcgc accgtggccc agcacaggac
     3541 cgccacccac gggaaccagg ccaccgtctg cacgatagtt acatgcccgg cgcccaggtg
     3601 gccgatcagg cggggcgtcg ccatgaaggc cagcgccgca accagggctg ccaggggcga
     3661 cagtcgcaac gccacgcgcc ccagcgccag cacgccgacc ccggccagca gcatgtgccc
     3721 ggcgagcag
//
LOCUS       DASUYC010000700         3892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1714536, whole genome shotgun sequence.
ACCESSION   DASUYC010000700 DASUYC010000000
VERSION     DASUYC010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1714536"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            264..1205
                     /gene="coxB"
                     /locus_tag="VF276_11485"
     CDS             264..1205
                     /gene="coxB"
                     /locus_tag="VF276_11485"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX6607524.1"
                     /translation="MPDPISPLARASTDLFNGTLFISFLILLGIGGFILYSIFRFRAR
                     PGDTTEPPAIYNNRRLELAWTAGPALILLGVLIFAIPAQLAQTVETSKPADGQEPPDI
                     QIIGHQWWWEIRYPQAGASVITANEVHMPVGKRLLIQVDSGDVIHDVWIPQLGPKMDT
                     VPGQTNWMWLEADRPGTYQGVCAEYCGTEHAWMLIRAIAQPQAEFDAWLQQQRAPAVA
                     PTTAETQHGAQLFAQRTCINCHAISGTAAQARVGPDLSHYASRGVLGSGVLPRTPENT
                     MRWLQNPQAVKPGNRMPNLHLAPDELRDLTAYMESLK"
     gene            1202..2863
                     /gene="ctaD"
                     /locus_tag="VF276_11490"
     CDS             1202..2863
                     /gene="ctaD"
                     /locus_tag="VF276_11490"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020601448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEX6607525.1"
                     /translation="MSAIPLVPEPTEALPAVRSDEGLISWIASVDHKQIGILYLITTI
                     GFFVVGGIEALLMRLQLAVPGNTLLQPGAYNQIFTMHGTTMVFLVVMPILIGFANYFV
                     PLMIGARDVAFPRLNAFSYWMLLFSGLLLYYSFIAGGAPDAGWFAYAPLSERPYSTTP
                     GLDYYTVGLLASGIGTVASAINLIVTILHLRAPGMTLRRLPLFVWMVLFNSVLIILVI
                     PALNAALAMLLIDRQLAAHFFDPLHGGHPLLWQHIFWSFGHPEVYIMVLPAFGMISEM
                     IPVFSRKPIFGYGFVAGSTIAIGFLSLLVYAHHMFTVGMGFTLDLAFGLASMLIAIPT
                     GVKIFNWTATMFRGRLHFTTAMKFAVAFLILFPMGGVTGVQFATVPVDKQLTDSYFVV
                     AHMHYVLFGGTAFAVFGAFYYWFPKMTGRLLSERLGNVHFWLAFIGFNMTFLIQHVLG
                     WDGMPRRVWTYPDNPGWGLMNMISTIGAFILAFSVLLFVINCVLSLRRGQIAGDNPWN
                     AWTLEWATTSPPPIHNFDQIPPIRGRRPLWDLAHPDAADWKKETH"
     gene            2877..3509
                     /locus_tag="VF276_11495"
     CDS             2877..3509
                     /locus_tag="VF276_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX6607526.1"
                     /translation="MTGIEHLNAGNAEVEAPHLDRFERLEPNQLAVALFIVSEVVFFA
                     MLIIAYIFYRDSPAVAGGPTAKRSLDIALTTLFSICLFASSGTIWFADRSLARGNEKG
                     VRLWLGATIVLGAIFIGGQGYEYSKLLSEDITLNTNLFGTTFYTLTGFHGLHVTAGLI
                     ALIILFVLALTGQFRNVTHNSAMAAISLYWHFVDVVWVVVFSVVYIWALF"
     gene            3509..>3892
                     /locus_tag="VF276_11500"
     CDS             3509..>3892
                     /locus_tag="VF276_11500"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX6607527.1"
                     /translation="MTVQRLLLTAWDWEPSVVLGCIALVLAYLAALHFRPSMAWVPFL
                     LGVGFLLLSLVSPIDVLGDRYLFSMHMLQHLLMIVVVPPLLLLGLPAELVRRMLAWRP
                     AGIVATASSNGLVAWLLAFTTLYVWH"
BASE COUNT          642 a         1309 c         1143 g          798 t
ORIGIN      
        1 taaaacttaa catgacatat aaatcacggg accccaaata cgcggcataa cgcaaagccc
       61 ggcggtctcg ttagaacaga cgccaggaac caacacgctc tcatgccgcg gttgttcttg
      121 tatttacctc taattgtagc cgcatagttg cccgctatcg actacggtga ttcaaaccgg
      181 cccatttgct cgtcacgcca accagagtac agccgaatag ttagagccaa acagcgccaa
      241 gcgctgcgcg caaaggatgt gacatgcctg atcccatctc gccgctcgcg cgtgccagca
      301 ccgacttgtt caacggaacc ctgttcatct cgttcctgat cctcctgggc atcggcggat
      361 tcatcctgta ctcgatcttc cgcttccggg cccggccagg ggatactacg gagccgccgg
      421 cgatctacaa caaccgccgc ctggagctcg cctggaccgc cggcccggcc ttgatcctgc
      481 tcggcgtgct gatcttcgcc atccccgcgc agctcgccca gaccgtcgag accagtaagc
      541 ccgcggatgg gcaggagccc cccgatatcc agatcatcgg gcaccagtgg tggtgggaga
      601 tccgttatcc ccaggccggc gccagcgtca ttaccgccaa cgaagtccat atgccggtcg
      661 gcaagcgctt attgatccag gtcgattccg gcgacgtgat ccacgacgta tggatcccgc
      721 aactcggccc caagatggac accgtgcccg gccagaccaa ctggatgtgg ttggaggccg
      781 accggcccgg cacctatcag ggcgtttgcg ccgagtactg cggcacagag cacgcgtgga
      841 tgctgatccg ggccatcgcc cagccgcagg ccgagttcga cgcctggctg caacagcaac
      901 gtgccccggc ggtggccccc acgaccgccg agacgcagca cggcgcacaa ctcttcgccc
      961 agcgcacgtg catcaactgc cacgcgatca gcggcacggc ggcccaggcg cgcgtcggcc
     1021 ccgacctcag ccactatgcc agccgcgggg tgctgggctc aggcgtcctg ccccgcaccc
     1081 cggagaacac catgcgctgg ttgcagaacc cgcaggcagt caagccgggc aaccgcatgc
     1141 ccaacctgca cctggccccg gatgagttgc gagatctcac ggcctatatg gagagcttaa
     1201 aatgagcgca attccgctgg ttcccgaacc caccgaagcc ctgccggctg ttcgctccga
     1261 cgaggggttg attagctgga tcgcgtcggt cgatcataag cagatcggca ttctgtacct
     1321 gatcacgacg atcggcttct tcgtcgtggg gggcatcgaa gccctcttga tgcggctgca
     1381 actggcggtg cccggcaaca ccctgctgca accgggggcc tataaccaga tcttcacgat
     1441 gcacggcacg accatggtct ttctggtcgt catgcccatc ctgatcgggt tcgccaacta
     1501 cttcgtgccg ttgatgattg gggcgcgcga cgtggctttc ccgcgcctaa acgccttcag
     1561 ctactggatg ttgctgttca gcggcctgct gctctactac agctttatcg cgggcggcgc
     1621 gcccgacgcc gggtggtttg cctacgcgcc cctgagtgaa cggccctact cgaccacacc
     1681 cggcctggac tactacaccg tgggcctgct ggctagcggt atcggcacgg tggcgtcagc
     1741 catcaacctg atcgtcacca tcctgcacct gcgcgcgccg ggcatgacgc tgcgtcggct
     1801 gccgctcttt gtctggatgg tgctgttcaa ttcggtcctg attattctcg tgatcccggc
     1861 gcttaacgcg gcgctggcga tgctgctcat cgaccgccag ttggccgccc acttcttcga
     1921 cccgctgcac ggcggccacc ccctactctg gcagcacatc ttctggtcct tcggccaccc
     1981 cgaagtctat atcatggtct tgccggcctt cggcatgatc tccgagatga tccccgtctt
     2041 ctcgcgcaag ccgatcttcg gctacggctt cgtggccggt tcgaccatcg ccatcgggtt
     2101 cttgagcctg ctggtctacg cgcaccacat gttcacagtg ggcatgggct tcacgctcga
     2161 cctggccttc ggcctcgcca gcatgttgat cgccattccc accggggtca agatcttcaa
     2221 ctggacggcg accatgttcc gcggcaggct gcacttcacc accgcgatga agttcgccgt
     2281 ggccttcctg atcctcttcc ccatgggcgg tgtcaccggc gtgcagttcg ccaccgtgcc
     2341 cgtcgataag caactgaccg acagctactt cgtggtggcg cacatgcact atgtgctgtt
     2401 cggcggcact gccttcgcgg tcttcggcgc cttctattac tggttcccca agatgaccgg
     2461 gcggctgctc tccgagcgcc taggcaacgt tcacttctgg ctggccttca tcggcttcaa
     2521 tatgaccttc ctgatccagc acgtgctggg ctgggacggc atgccgcgcc gggtgtggac
     2581 ctatcccgac aatccgggct ggggcttgat gaacatgatc tcgaccatcg gcgcgttcat
     2641 cctggccttc tcggtgctct tgtttgtcat caactgtgtg ctgagcctgc ggcgcggcca
     2701 gatcgccggg gacaatccct ggaacgcctg gaccctggaa tgggccacga cctcgccgcc
     2761 gcctatccac aacttcgacc aaatcccgcc catccggggc cgccggcctc tctgggacct
     2821 ggcccacccg gacgccgccg actggaagaa agagacgcat tagggggagt cacgccatga
     2881 caggcattga acacctgaac gccgggaacg cggaagtgga agcgccgcat ctcgaccggt
     2941 ttgaacggct ggaaccgaac caactggcgg tcgccctctt cattgtgtcc gaagtggtct
     3001 tcttcgccat gctgatcatc gcctatatct tttaccggga ctcgcccgcc gtggccggcg
     3061 gccccacggc gaaaagatcc ctggatattg cgttgaccac gctgttcagc atctgcctgt
     3121 tcgccagtag cggcacgatc tggttcgccg accgcagcct ggcccgcggc aacgagaagg
     3181 gtgtgcgtct ctggttgggc gcgacgatcg tcctgggcgc catcttcatc ggcgggcagg
     3241 ggtacgagta cagcaagttg ctcagcgagg acatcacgct gaacacgaac ctgttcggca
     3301 ccaccttcta tacgctgacc ggcttccatg gcctgcacgt gaccgccggg ctgatcgcgc
     3361 tgatcattct gtttgtcctg gccttgacgg gccagttccg caacgtcacg cacaactcgg
     3421 ccatggccgc gatctcgttg tactggcact ttgtcgacgt ggtgtgggtg gtggtctttt
     3481 ccgtcgtcta catctgggcg ttgttctgat gacagtgcag cggctgctac tgacggcgtg
     3541 ggattgggaa ccctccgtgg tgctggggtg catcgcgctg gtgctggcct atctggcggc
     3601 cctgcacttc cggcccagca tggcctgggt gcccttcctg ctgggggtgg gcttcttact
     3661 gctcagcctc gtctcgccca tcgacgtgct gggcgaccgc tacctgttca gtatgcacat
     3721 gctgcagcac ctgttgatga tcgtggtggt gccgccgctg ctgctgctgg ggctgccggc
     3781 ggagctggtg cggcggatgc tggcctggcg cccggccggg atcgtcgcca cggccagcag
     3841 caacggtctg gtggcctggt tgctcgcctt caccacgctg tatgtttggc ac
//
LOCUS       DASUYC010000701         2271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1827409, whole genome shotgun sequence.
ACCESSION   DASUYC010000701 DASUYC010000000
VERSION     DASUYC010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1827409"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="VF276_11505"
     CDS             <1..1107
                     /locus_tag="VF276_11505"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6607528.1"
                     /translation="DRAADHLACFLGEVPLRDGALGGRERRGMMSEQWYTVNWSREGR
                     AGEAAPVSHAWCLGLLLFAADDWLGDLTLPTGNEKKKVMPQSTDTPLIPGVVVERPRT
                     KRHPAGSARTRGPFLPPVPDHPHPVRLLPGFYQIGGGYLSHPRDAASYLLLDEASGDS
                     IMVDCGSHSGLAALRNNVNQVADLGKVRMVIGTHGHWDHVEAFGHLRSEMDARFAIHE
                     RDADAVRNGDPDLTCAGFLYNEPFDPFEIDILLKGGEHFTIGDYMLEILHLPGHSPGS
                     IGVLLHYARTDQTILIPGDAVQGAFGRQIHSNLRSWKKSMQRLMQDPPDLMVPNHLPG
                     NAQTALLADVPNRLARIYSQLQTDFLNFMDHQRT"
     gene            1121..>2271
                     /locus_tag="VF276_11510"
     CDS             1121..>2271
                     /locus_tag="VF276_11510"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607529.1"
                     /translation="MLTGIVVNHTHWDREWYMPFQRYRVLLVDAVDKVLDLLAHQADY
                     GSFMLDGQTVIVEDYLEVRPEQRERLLSFIHSGRLSVGPWYVLPDEFIPSGESLIRNL
                     LLGRRQMRALGAPPARVGYLPDTFGHPAQLPQILAGCDLDSMVIFRGVKVDTSEFLWR
                     APDGTRLLTIYLPGGYYNAMELARAPEYWLAEKLPAMIEQAARFATVDTVLIMNGCDH
                     FEPQPATQAILDRANALQDTIHLQQGTLAEFVERVQPHAARLPVLEGEWRYNRPARIT
                     PGVLSARMYLKQANFRGETSLERGAEPLGALAWALTGHEDTGLLRTAWRYILQNHPHD
                     SICGCSVDAVHRDCEARFRWAEEIGGDLTERGATRVAGRIGGAGPGFAL"
BASE COUNT          370 a          825 c          736 g          340 t
ORIGIN      
        1 gaccgggcgg cggatcacct ggcctgtttc ctcggcgaag tcccgctccg cgacggcgcg
       61 ttgggcgggc gcgagcgccg ggggatgatg agcgaacaat ggtatacggt gaactggtca
      121 cgcgagggcc gcgccgggga ggcggcgccg gtctcgcacg cctggtgcct gggcctgctg
      181 ttatttgccg ccgatgattg gctgggcgat ctgacgttgc ccaccggaaa tgagaaaaag
      241 aaagtcatgc cgcaatctac cgacacacca ctgatccccg gcgtcgtggt cgaacgcccg
      301 cggaccaaac gccaccccgc cggcagcgcc cgcacgcgcg ggccgttcct gccgcccgtg
      361 cccgaccatc cgcatcccgt acgtctgctg ccgggcttct accagatcgg cggcggctac
      421 ttgagccacc cgcgcgacgc cgccagctac ctgctgctcg acgaggcctc cggcgattcg
      481 atcatggtcg actgcggcag ccacagcggg ctggccgccc tacgcaacaa cgtgaaccag
      541 gtcgccgacc tgggcaaggt gcgcatggtc atcggcacgc acggccactg ggaccatgtc
      601 gaagccttcg gccacctgcg cagcgagatg gacgcccgct tcgccatcca tgagcgcgac
      661 gccgacgccg tgcgcaacgg cgaccccgac ctgacctgcg ccgggttcct ctataacgag
      721 ccgttcgacc ccttcgagat cgacatcctc ttgaagggcg gcgagcactt caccatcggc
      781 gactacatgc tggagatcct gcacctgccg ggccactcgc ccggctccat cggcgtgttg
      841 ctgcactacg cgcgcaccga tcagacgatc ctgatccccg gcgacgccgt gcagggcgcc
      901 tttgggcggc agatccactc caacctcagg tcctggaaga agtcgatgca acggctgatg
      961 caagacccgc ccgacctgat ggtgcccaac catctgccgg gcaacgcgca gaccgccctg
     1021 ctggccgatg tgcccaaccg cctggcgcgc atctacagcc agttgcagac cgacttcctg
     1081 aacttcatgg accaccagcg gacgtgagga ctcgcctgta atgctcaccg ggatcgtcgt
     1141 caaccatacc cactgggacc gcgagtggta catgccgttc cagcgctacc gcgtgctgct
     1201 cgtggacgcc gtggataagg tgctggatct cctggcccac caggccgact acggcagctt
     1261 catgctcgac ggccagacgg tgatcgtcga ggactacctg gaggtgcggc ccgagcagcg
     1321 cgagcggctg ctgagcttca tccatagcgg gcggctcagc gtcggcccgt ggtacgtgct
     1381 gcccgacgag ttcatcccca gcggcgaaag cctgatccgc aacctgctgc tgggccgccg
     1441 ccagatgcgc gcgctgggcg cgccgcccgc ccgcgtcggc tacctgcccg acacgttcgg
     1501 ccacccggcc cagttgccgc agatcctggc cggctgtgac ctcgacagca tggtcatctt
     1561 ccgcggcgtg aaggtcgata ccagcgagtt cctctggcga gcgcccgacg gcacccgtct
     1621 gctgaccatc tacctgcccg gcggctacta caacgccatg gaactggccc gcgcccctga
     1681 atactggctg gccgagaagc tgccggccat gatcgagcag gctgcccgct tcgccacggt
     1741 ggacaccgtg ctgatcatga acggctgcga ccactttgag ccgcagcccg ccacacaggc
     1801 catcctcgat cgggccaacg cgctgcaaga caccatccat ctccagcagg gcacgctggc
     1861 cgagttcgtg gagcgggtgc agccccacgc ggcgcgcctg ccggtgctgg agggcgagtg
     1921 gcgctacaac cgcccggcgc gcatcacgcc tggcgtgctg tcggcgcgga tgtacctcaa
     1981 gcaggccaac ttccgcggcg agaccagcct ggagcgcggc gccgagccgc tgggcgcgct
     2041 ggcctgggcg ctgaccggcc acgaagacac ggggctgctg cgcaccgcct ggcgctacat
     2101 cctgcaaaac cacccgcacg actccatctg cggctgcagc gtggacgccg tgcatcgcga
     2161 ctgcgaggcg cgcttccgct gggccgagga gatcggcggc gacctgaccg agcgcggagc
     2221 cacgcgggtg gcgggccgca tcggcggcgc ggggccgggc ttcgccctgt t
//
LOCUS       DASUYC010000702         2685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1680868, whole genome shotgun sequence.
ACCESSION   DASUYC010000702 DASUYC010000000
VERSION     DASUYC010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1680868"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(317..772)
                     /locus_tag="VF276_11515"
     CDS             complement(317..772)
                     /locus_tag="VF276_11515"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6607530.1"
                     /translation="MITTRVTVEIARPIGEVFAFVTTPANFPRWAGAVVTASRQTSPG
                     PVGVGTTFTQQNRLLGRRFTTALRVVTYEPDRRFEYESTAGPIRFAGHYTFAPTPGGT
                     RFTSVDMTTPPGVLRVLQPLLQPLAQRQIARNLARLKAVLEAGAGVPGP"
     gene            1076..1795
                     /locus_tag="VF276_11520"
     CDS             1076..1795
                     /locus_tag="VF276_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607531.1"
                     /translation="MLSAIAGPAARRRGTWWPGLLTTGAATLAPAALLARRVPGRPLW
                     AGAALAPWAYLLALRPRQLRWGATPAEVAAPLPGDEIVPRPTVEATRAITIAAPPQAV
                     WPWIVQIGYGRAGFYSYAPVERALGLHGLHNADAILPEFQHLAVGDVVPQTPAAGWTV
                     RALAPPHTMVWAADAVSSWTWALAPLDGGWTRLVVRTRIRYPRTPLWAVFFVLQEAGG
                     EFLMLRRMLLGLKDRAERSAE"
     gene            2148..2558
                     /locus_tag="VF276_11525"
     CDS             2148..2558
                     /locus_tag="VF276_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607532.1"
                     /translation="MLKDYNTAAPPSPLPAPGLWRWVRDPAPATVPIWANRILRLLLA
                     APLLWWLYGMVSALVWRLDPAYSGIMAGVPDDLIQADMLFVQVNIGAAALGLAVLALW
                     RWQPRIARGLLLGFGIAVLVTLCAFFNVLSHSRI"
BASE COUNT          338 a          988 c          960 g          399 t
ORIGIN      
        1 atgccgaatt gcgcgctcac tggatcgccg ccggtgctcc gtggggcagc cgctacaccc
       61 tcccgccgcc gcactggtcc ccggtggaac aactacgcaa catcgggtac acggtgacgg
      121 agagcgatct gcgcgccatc gcccagcccc ccgcccccac gccgccctga accggctccg
      181 ccgtaccgcc ccggtcccta ctgccgtagg ggcggaccca cgtgtttgcc cggcggcctg
      241 accactcctg ctgcccctcg gccccgctcc ccgatccagc ccgcgcagga gggcttcgcc
      301 gccctagccc gcccggtcac gggcctggca ctcccgcgcc cgcttcgagg acggctttca
      361 gccgggccag gttgcgcgcg atctggcgct gggccagcgg ctgcaacagc ggttgcagca
      421 cccgcagcac gcccggcggc gtcgtcatgt ccaccgaggt aaagcgcgtg ccgcccggcg
      481 tcggggcgaa ggtgtagtgc ccggcgaagc ggatgggccc cgccgtggac tcgtactcga
      541 agcggcggtc cggctcgtag gtgacgacgc gcagggcggt ggtgaagcgg cggcccagca
      601 gccggttctg ctgggtgaac gtggtgccca cgcccaccgg gccgggcgag gtctggcgcg
      661 aggcggtgac caccgcgccg gcccagcgcg gaaagttggc cggggtggtc acgaaggcga
      721 agacctcgcc gatcgggcgg gcaatctcga cggtgacacg ggtggtgatc atacggcggc
      781 tcctcacgaa cgtagccggc ccccggcctg cccggcggcc ccctggtgct cctactgtag
      841 cacggcccgc cgccacccgc cagcccaaaa cctgctcact ttttggcgcc gcggcggccc
      901 gcccggcccc ggtagccatc ccttcacccg ccgtgcacga agcgcacccg gcgcggcggg
      961 ccggggatgc taggatgaac agtgggggcg tgggtgcgcg ggggataacg agtccacccc
     1021 cgcccgcgct cccaccgcgc ggcccccatc cggtcgccgc ctggaggtgc gacctatgct
     1081 gtccgcgatt gccgggcccg ccgcccggcg ccggggcacc tggtggcccg gcctgctgac
     1141 cacgggggcc gccacgctgg ccccggcggc gctgctggcc cggcgcgtgc cggggcggcc
     1201 cctctgggcc ggcgctgccc tcgccccatg ggcctatctg ctggcgctgc ggccccgcca
     1261 gttgcgctgg ggcgccaccc ccgccgaggt ggccgcgccc ctgcccggcg atgagatcgt
     1321 gccccgcccc accgtcgaag ccacccgcgc catcaccatc gctgccccgc cgcaggccgt
     1381 ctggccctgg atcgtgcaga tcggctatgg ccgggcgggc ttctacagct atgccccggt
     1441 cgagcgcgcg ctgggcctgc acggcctgca caacgccgat gcgatcctgc ccgagttcca
     1501 gcacctggcc gtgggcgatg tggtgccgca gacgcctgcc gccggctgga ccgtgcgcgc
     1561 gctggccccg ccgcacacta tggtctgggc cgccgacgcc gtgtcgagct ggacgtgggc
     1621 gctggccccg ctggatggcg ggtggacccg cctggtggtg cgcacgcgca tccgctaccc
     1681 gcgcacgccg ctgtgggccg tcttcttcgt gctgcaggaa gccggcggcg agttcctgat
     1741 gctgcggcgg atgctgctgg gcctcaagga ccgcgcggag cggagtgctg agtgacggcg
     1801 agtggtcggt ggtcggtggt cagccgtcag ccgtcagccg tcagccgtca ggagacgagt
     1861 gacggatggg caggggtcgg gaggccgtag tgagtcgcaa ttgtcactct gagcgcagcg
     1921 aagagtctcg tcgttcgccc caacgcgacg ggattcttcg ctgcgcgcag aatgacagga
     1981 tgcggggcgg ttgtcactct gagcagagcg aagagtctcg tcgttcgccc caacgcgacg
     2041 ggattcttcg ctgcgcgcag aatgaccgca gctactgacc cctgacccct gaccactgac
     2101 cactgaccac tcgtccccgg ccccggttcg gacaggaggg agcaagcatg ctgaaggact
     2161 acaacactgc cgcgccgccc agcccgctgc ccgcgccggg gctgtggcgc tgggtgcgcg
     2221 atccggcgcc ggcgaccgtg ccgatctggg ctaaccgcat tctgcggctg ctgctcgccg
     2281 cgcccctgct ctggtggctc tacggcatgg tgtcggcact ggtgtggcgc ctggaccccg
     2341 cgtatagcgg gatcatggcg ggtgttcctg atgacttgat ccaggcggac atgttgttcg
     2401 tgcaggtcaa tatcggcgca gccgcgctcg gcctggcggt gctggccctg tggcgctggc
     2461 agccacgcat cgcgcgcggc ctcctgctcg ggttcggcat cgcggtgctg gtcaccctgt
     2521 gtgccttctt caacgtgttg agccactcgc gcatctagcg ccgccgggca acccgcagtc
     2581 gcgcctctcg ccgcgcggcg gggtataccc ccaacggggg agaggttggt gaggcaagac
     2641 aagtcgccgt cccgcggcgg ggaggggtag ggagcggggt ttacc
//
LOCUS       DASUYC010000703         2451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_962848,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000703 DASUYC010000000
VERSION     DASUYC010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962848"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2451)
                     /locus_tag="VF276_11530"
     CDS             complement(<1..>2451)
                     /locus_tag="VF276_11530"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEX6607533.1"
                     /translation="LLAPLLAPAPAAAQTTQRQTWDSVDVAITVNPDGTFEVTERQVF
                     SFDQGTFRGGFRDIDESRMTDLTNVSVSEDGLGAYRPDNTPINLNTGKLGPPSTFQVL
                     DQGGSKRIIWFYNGPVNAPAKKTMVIKYTVVGGLRYYDSGDQLWWSPFLPDRSAGRIN
                     QARLTVQVPSNIDLTASGANAVTFPADAGTVQVSPQQAIFTANGPLDPSDQFEAQLTW
                     PHGAIPGTAPPWQVADDRRRAAQEQANLRQSWDSDDVTIAIQPDGTFNVTERQVFLFQ
                     QGTFRGSFRDIDTSRMTGLSNIGVSEEGVGDYRLDNTPVDTASGKYGPPHTYQVQDQP
                     SAKRIVWFYEPVTAPAKKTMVIKYTVAGGLRYYTGGDQLWWSPFMPDRNATINQPRLT
                     VQMPPNVNLTDPAVKIAVNPANSGTFQVTPQQVVFTFDSLGPRENVEARIQWPHGLIA
                     GSPAPWQAADDKRVADEAAAALAEQQRRGRAILITGGIGLLGLVGGTLFWIWRWYSGG
                     RDPVTGPIADYLNEPPSDLPAGLVGTLLDEKTDVRDVIATILDLGRRGNLRIEEVRVP
                     DGGLFGGEHSDFQYTFLNGEVRYAFERRVLAALFDAHGSPVRLSDLKNEFYSKLPGIY
                     AEMYDAMVQTGLFPQNPEAVRGGYRALAAGFAIVGVLLFCGLPMFGLDSAAMMFIPFA
                     FGIPAVLGFVLAGVMPRKTPAGADQAARWRAFGRYLADLQRFGDVNQAAQRFGDFLPY
                     AVAMGVDQQYTRQFESVQGQMPMPTYYYPWGWVPGYGYGYGVPGVPGARGLAGAGVVG
                     AGGTIDSGSRGGIDFSPG"
BASE COUNT          353 a          808 c          896 g          394 t
ORIGIN      
        1 cgcccggcga gaagtcgatg ccgccgcgcg acccggagtc tatggtaccg cccgcgccca
       61 cgacgcccgc gccggccaga ccacgcgcac ccggcacgcc cggcacgccg tagccgtaac
      121 cgtagcccgg cacccagccc cagggataat agtaggtggg catcggcatc tggccctgca
      181 cgctctcgaa ctggcgggtg tactgctggt ccacgcccat ggcgacggcg tagggcagga
      241 agtcgccgaa gcgctgggcg gcctggttca cgtcgccgaa gcgttgcagg tcggccaggt
      301 agcgcccgaa ggcgcgccag cgcgcggcct gatcggcgcc cgccggggtc ttgcgcggca
      361 tcaccccggc cagcacaaag ccgagcacgg cggggatgcc gaaggcgaac gggataaaca
      421 tcatcgccgc gctgtccagg ccgaacatgg gcagaccgca gaagagcagc accccgacga
      481 tagcgaagcc cgcggccagc gcccggtagc cgccgcgcac cgcctccggg ttctgcggga
      541 acaggccggt ctgcaccatg gcgtcgtaca tctcggcgta gatgccgggc aacttggagt
      601 agaactcgtt tttcaggtcg gaaagccgca ccgggctgcc gtgcgcgtcg aacagggccg
      661 ccagcacccg ccgctcgaag gcgtagcgga cctcgccgtt gaggaaggtg tactggaagt
      721 cgctgtgctc gccgccgaag aggccgccat cgggcacccg cacctcctcg atccgtaggt
      781 tgccgcgccg gcccaggtcc aggatggtgg cgatcacgtc gcgcacatcg gtcttttcgt
      841 ccagcaacgt gccgaccagc ccggcgggca ggtccgacgg cggctcgttc aggtagtcgg
      901 cgatggggcc ggtgaccggg tcgcgcccgc cgctgtacca gcgccagatc cagaacagcg
      961 tgccgccgac cagcccaagc aggccgatgc cgccggtgat gaggatggcc cggccccggc
     1021 gctgctgttc ggccagggcg gcggcggcct cgtcggccac gcgcttgtcg tcggcggcct
     1081 gccagggggc cgggctgccg gcgatcaggc cgtgcggcca ctggatgcgg gcctcgacgt
     1141 tctcgcgcgg ccccagcgag tcgaaggtga agaccacctg ctgcggcgtc acctggaacg
     1201 tgccgctgtt ggccgggttg accgcgatct tcaccgccgg gtcggttaga ttgacgttgg
     1261 gcggcatctg caccgtcagg cgcggctggt tgatcgtcgc gttgcggtcg ggcatgaagg
     1321 ggctccacca gagctgatcg ccgccggtgt agtagcgcag gccgccggcc acggtgtact
     1381 tgatgaccat ggtcttcttg gcgggcgccg tgaccggttc gtagaaccag acgatgcgct
     1441 tggccgacgg ctggtcctgc acctggtagg tgtgcggcgg gccgtacttg ccgctcgcgg
     1501 tatcgaccgg ggtgttgtcc agccggtagt cgcccacgcc ctcttcgctg acgccgatat
     1561 tgctgaggcc ggtcatgcgg ctggtgtcga tgtcgcggaa gctgccgcgg aaggtgccct
     1621 gctggaagag gaacacctgc cgctcggtca cgttgaaggt gccgtccggc tggatggcga
     1681 tggtcacgtc gtcgctgtcc cacgactggc gcaggttggc ctgttcctgg gcagcgcggc
     1741 gccggtcgtc ggcgacctgc cacggcggcg ccgtgccggg gatcgcgccg tgcggccagg
     1801 tcagttgggc ctcaaattga tcgctggggt ccagcgggcc gttcgcggtg aagatggcct
     1861 gctgcgggct gacctgcacg gtgcccgcat cggcggggaa tgttaccgca ttggccccgg
     1921 atgcggtcag gtcgatgttc gacggcacct gcacggtcag ccgggcctgg ttgatgcgcc
     1981 cggcgctgcg gtcaggcagg aaggggctcc accagagctg atcgccgctg tcgtagtagc
     2041 gcagcccgcc gaccacggtg tacttgatga ccatggtctt cttggcaggc gcgttgaccg
     2101 ggccgttgta aaaccagatg atgcgtttgg agccgccctg gtccagcacc tggaacgtgc
     2161 tcggcgggcc gagtttgccc gtgttcaggt taatcggcgt gttgtcgggg cgatacgcgc
     2221 ccaacccgtc ctcgctgacg ctcacattgg tcaggtcggt catgcggctc tcgtcgatgt
     2281 cgcggaagcc gccgcggaag gtgccctggt cgaacgagaa gacttgccgc tcggtcacct
     2341 cgaaggtgcc gtccgggttc acggtgatgg cgacatcgac gctgtcccac gtctggcgct
     2401 gcgtggtctg ggccgcggcg ggcgcggggg cgagcagggg cgcgagcagg g
//
LOCUS       DASUYC010000704         2235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_707032,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000704 DASUYC010000000
VERSION     DASUYC010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707032"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            329..>2235
                     /locus_tag="VF276_11535"
     CDS             329..>2235
                     /locus_tag="VF276_11535"
                     /inference="COORDINATES: protein motif:HMM:NF026092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HEX6607534.1"
                     /translation="MITDRTGNIGHELQGLYEQDTRLLSRYTLLVNGSPPRLDALSGV
                     DAYSTLGYYVSPPAPDAQIPRDALGLAQGERDRQVVIRVARFVGRGMHEDLEVTNHGL
                     DAADLEIAWEVDADFADLMEARGGARRQSAPVEVDWQVTGDGDGLLRFDYQHPQLRRG
                     VLCRWHPKNGPESAPPSPSYAGHRISVALHLRPQETARYCLTIAPIIEGQVLAPLYGC
                     DSFGAAGAGAATRPPVDRAVGTWRARAARLSTPNPLVQHAWNRAASDLEALALGDGQT
                     PAEWAVPAAGMPLYGTLFGRDALTLAGQSLLLSPDLAEGALRLLARHLGTHDDDFYDE
                     QPGRVPQQVRAGPLALLGLTPWLHDYGDYAAPCAYLVLLGGYHLVTGDGSRVREFLDP
                     AKRVLDWLDERADLDGDGFLEYKTRSPNGQKHQGWKDSGDAVRYEDGREVEPPVAACE
                     IQGYWYAAKLLMAEVFLHVGEGARALEQLRAAAALKRRFNERFWMPEERFFAFALDSD
                     KRQVKSIVSNVGHCLATGIIEHRYARDVVRRLMAPDMFSGWGIRTLSARHPAYNPLKY
                     HLGSVWPVENATTAFGMKRYGFHRECNALARGMFEAAALFAHYRLPETFGGHPRDRAH
                     PHPGIYPDACAP"
BASE COUNT          346 a          748 c          790 g          351 t
ORIGIN      
        1 ggcatgttcc ccaagacgcc gggccggatc aggccggccc ccggcgggcc gccatgggga
       61 tcgccatccg ccggcgccgg tacgcctgcg ggcgcggaag gaggcgcccg ccccgcgcgt
      121 cttcagcgtg gtggtgcgag atgtgctcac agactactct gcccagccag tatgcggtcg
      181 cagcgacacg accatgctcc tcgctggagc gcgggaaccg taacgccggc ccaggcgggc
      241 aaatgcgtgg ggagggaacg ccttgatctc cgagatctta cttagcgtct atccggatct
      301 gcgattcgct tatcgggggc attcgctctt gatcacggac cgtacgggaa acatcggaca
      361 cgagctgcaa gggctatatg agcaagacac ccgcctgctg tcgcgataca cgctgctggt
      421 caatgggagc ccgccccggc tggacgccct cagcggggtc gatgcgtatt ccacgttggg
      481 ctactatgtg agcccgccgg ccccggacgc gcagatcccg cgggatgccc tggggctggc
      541 gcaaggcgag cgggaccggc aagtggtgat ccgggtcgcg cgtttcgtgg gccgggggat
      601 gcatgaggac ctggaggtga ccaaccacgg cctggatgcg gccgacctcg aaatcgcctg
      661 ggaggtggac gccgacttcg ccgacctcat ggaggcgcgc ggcggcgcgc gccggcaatc
      721 ggccccggtg gaggtcgact ggcaggtgac aggcgatggc gatggcctgc tccgcttcga
      781 ttatcagcac ccgcaactgc ggcggggcgt cctgtgtcga tggcacccga aaaacgggcc
      841 ggaatcggcg cccccgagcc cgagttatgc agggcaccgc ataagcgtcg cgttacacct
      901 gcggccgcaa gagaccgcac gctattgcct gacgattgcg ccgattatag aggggcaggt
      961 gttagcgccg ctctatgggt gcgattcgtt tggggcggca ggcgccggcg ccgcaacccg
     1021 gccaccggtc gaccgggcgg tgggcacatg gcgcgcgcgc gccgcccgcc tgtctacgcc
     1081 taacccgctc gtgcaacacg cctggaaccg ggccgcatcc gatctggagg cgctggcgct
     1141 aggcgacggg cagactccgg cggagtgggc cgtgcccgcg gccggcatgc cgctgtatgg
     1201 cacgttgttc gggcgcgatg ccctcaccct ggcgggccaa tcgctgctgc tttcgccgga
     1261 cctcgcggaa ggggcgctcc ggttgttggc gcgccacctg ggcactcacg atgatgattt
     1321 ctatgacgag cagccggggc gcgtcccaca gcaggtgcgc gccggtcccc tggcgctgct
     1381 cggcctcacg ccctggctac acgactacgg cgactacgcc gcgccctgcg cgtacctggt
     1441 gctcctgggg ggctaccacc tggtgacggg cgacggcagc agggtgcgcg aattcctgga
     1501 tccggccaaa cgggtgctcg actggctcga tgagcgggcc gacctggatg gcgatgggtt
     1561 cctggagtac aagacccgct cccccaacgg gcagaaacat caggggtgga aggactccgg
     1621 cgatgccgtg cgctatgaag atggccggga ggtcgagccg ccggtcgccg cctgcgaaat
     1681 ccagggctac tggtacgcgg ccaagctctt gatggccgag gtcttcctgc acgtgggcga
     1741 aggcgcgcgg gcgctggagc aactacgggc cgcggcagcg ctgaagcgcc gcttcaacga
     1801 acgcttctgg atgcccgagg aacgcttctt tgccttcgcg ctcgattccg acaagcggca
     1861 ggtgaagtcg atcgtgtcca acgtcgggca ctgcctggcg acgggcatta ttgagcaccg
     1921 gtatgcccgc gacgtggtcc gccggctgat ggcgccggat atgttctcgg gctggggcat
     1981 ccgcacgctg tcggcccgcc accccgccta caacccgctg aagtatcacc tgggctcggt
     2041 ctggccggtg gagaacgcga ccacggcgtt cggcatgaag cgctatggct tccaccgcga
     2101 atgcaatgcc ctggcgcgcg ggatgttcga ggcggcggcc cttttcgccc actaccggct
     2161 gccggagacg tttggcgggc acccgcgcga ccgggcccac ccccaccccg gcatctaccc
     2221 cgacgcctgc gcgcc
//
LOCUS       DASUYC010000705         2627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1494263, whole genome shotgun sequence.
ACCESSION   DASUYC010000705 DASUYC010000000
VERSION     DASUYC010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494263"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2612)
                     /locus_tag="VF276_11540"
     CDS             complement(<1..2612)
                     /locus_tag="VF276_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607535.1"
                     /translation="MQAALSSQPALDELQTRAEDDPTLALLDAWATVADVLTFYQERI
                     ANEGYLRTATERRSVLELARAIGYELNPGVAASTVLAFTVEDAPSQLNAATTAQTNLA
                     AAGQAVEDDANAPSTATVPLGTRVQSIPAQGKLPQTFETIEAILARAEWNALRPRQTQ
                     PQKLAISGTTLQLVGLDGTKITAQQLYLAGTGTNLKPGDLLLVAVRDSAGALQSLPLA
                     VRAVVPETALNRTRVDLAANLSGTPVLPAVSPPGPAQADSPPAVQGAPPALNATNVNT
                     YVLSRRWRESDLSAFLTAQSWSVRKVARVANQPVVVTTTQPLDGVFAFRARLGFFGHN
                     APAYNSLPAPGDVLRGEPYPNDWDAGNGWEIWKDPNKTSFNIFEQAKSTSFTLRNLDT
                     PAGADTRAVVIGGNPSKDIAIGGTVNPYWDNAHVYLERTVQGLVGSSWAVFELPTALY
                     TVYQIDKANETSLAAFSVSGKAAGLLLKNKDGSTLANSNKTASLTVRKTTAYVQSERL
                     TLIDLPIQEPIAAGATALVLDRLVPGLQVGRQLILSGGRADLPGVSSSEVVTLADIIH
                     DHGYTTLYFASALQYSYTRESIVLTGNVARATHGETVREALGNGDGSVANQRFVLKKP
                     PLTYVSAPTPSGGKSTLSVRVDGLLWQEVFSLYGLAPRSENYIVRIDDDGKATVIFGD
                     GKQGARLPTGQENVVATYRSGIGPDGEVSAGSLTLLQTRPLGIRGVTNPLAASGAQAP
                     EQLADARANAPLTVLTLDRIVSLQDFEDFARAFAGIGKAQATLLWNGQTQQVHVTIAS
                     ASGTVVDATSDLFKNLRTAMDAARDPLQHLEIVTFRPRTFSIDAGILVDAPRYVAADV
                     LAGATTALQTAFAFA"
BASE COUNT          356 a          832 c          971 g          468 t
ORIGIN      
        1 gcaaaggcga aagccgtctg cagcgcggtc gtggccccgg ccagcacatc ggcggctaca
       61 tagcgcggcg catcaaccag gatcccggcg tcgatgctga acgtgcgtgg gcggaaggtg
      121 acgatctcca ggtgttgcag cgggtcgcgg gcggcatcca tcgccgtgcg caggttcttg
      181 aacagatcgg acgtggcatc gaccaccgtc ccgctggccg aagcaatcgt gacgtggacc
      241 tgctgggtct ggccgttcca cagcagggtc gcctgggcct tgccgatccc ggcgaaggcg
      301 cgggcaaaat cttcgaaatc ttgcagcgag acgatacggt ccagggtcag cacggtgagc
      361 ggcgcgttgg cccgcgcgtc ggccagttgc tcgggcgcct gggcaccgct cgcggccagt
      421 gggttggtca caccgcggat gcccagcgga cgggtttgca gcagggtcag gctgcccgcg
      481 ctgacctcgc cgtcggggcc gatgccgctg cggtaggtgg cgacgacgtt ttcctggccg
      541 gtgggcagcc gggcgccctg cttgccgtcg ccgaagatga cggtcgcctt gccgtcgtcg
      601 tcgatgcgca cgatgtagtt ctcgctgcgc ggggcgaggc cgtacaggct gaacacctcc
      661 tgccagagca agccgtccac gcgcacgctg agcgtgcttt tgccgccgct gggcgtgggc
      721 gcggagacgt aggtcagggg cggctttttg agcacgaagc gctggttcgc cacgctgccg
      781 tcgccgttgc ccagcgcctc gcgcaccgtc tcgccgtgcg tcgcccgcgc cacgttgccg
      841 gtgagcacga tgctctcgcg ggtgtagctg tactgcaagg ccgatgcgaa atagagcgtg
      901 gtgtagccgt ggtcgtggat gatgtcggcc agagtgacga cctcgctgct gctgacgccg
      961 ggcaggtcgg cccgcccgcc gctgaggatc aactggcggc ccacctgcaa gcccggcacc
     1021 agcctgtcga gcaccagcgc cgtggcgccc gccgcaatcg gttcctggat cggcagatcg
     1081 atcagggtca gccgttcgct ctgcacatag gcggtggttt tgcgcacggt caggctcgcc
     1141 gttttgttgc tgttggcgag cgtgctgccg tccttgttct tgaggagcag cccggcggcc
     1201 ttgccgctga cgctgaaggc ggccagcgac gtttcgttgg ccttgtcgat ctggtagacg
     1261 gtgtagaggg ctgtcggcaa ctcgaatacc gcccaactgc tgccgacgag gccctgcacg
     1321 gtgcgctcca ggtagacgtg ggcgttgtcc cagtagggat tcaccgtgcc gccaatggcg
     1381 atgtctttgg acggattgcc gccgatgacg accgcccgtg tatccgcgcc ggcgggcgtg
     1441 tccaggttgc gcaaggtaaa ggacgtggat ttcgcctgct cgaagatatt gaaactggtt
     1501 ttgttgggat ccttccagat ttcccagccg ttgccggcgt cccaatcgtt cggatagggt
     1561 tcgccgcgca gcacgtcgcc gggggcgggc aggctgttgt aggcgggcgc gttgtggccg
     1621 aagaagccca gccgggcgcg gaaggcaaac acgccgtcca gcggctgcgt ggtggtcacg
     1681 acgaccggct ggttggcgac gcgggcgacc ttgcggacgc tccagctctg cgccgtcagg
     1741 aaggcgctca ggtcggactc gcgccagcgg cgggagagga cataggtgtt cacgttcgtt
     1801 gcgttcaacg cgggtggcgc gccttgcacg gcgggtgggc tgtcggcctg ggcggggccc
     1861 ggcgggctga ccgcgggcag caccggcgtg ccgctcaggt tggcggccag gtccacgcgg
     1921 gtgcggttca gcgcggtttc gggcacaacc gcgcgcacgg ccagcggcaa gctctgcaag
     1981 gccccggcgc tgtcgcgcac ggccaccagc aacaggtcgc ccggcttgag gttggtgccg
     2041 gtgcccgcca ggtagagttg ctgcgcggtg atcttggtgc cgtccagccc caccagttgc
     2101 agcgtggtgc cgctaatcgc cagcttctgc ggctgggttt gccgcgggcg cagagcgttc
     2161 cactcggcgc gggccaggat ggcctcgatg gtctcaaagg tctgcggcag cttgccctgg
     2221 gccgggatgc tctgcactcg cgtgcccagc ggcacggtgg ccgtgctggg cgcgttcgcg
     2281 tcgtcttcca cggcctgccc ggcggcagcc aggttggtct gcgcggtggt ggcggcgttc
     2341 aactggctcg gcgcgtcctc caccgtgaag gccaggacgg tgctggccgc gacaccggga
     2401 ttgagttcat agccgatggc gcgggccagc tccagcactg agcgccgctc cgtcgccgtg
     2461 cgcaggtagc cttcattggc gatgcgctcc tggtagaagg tgagcacatc ggccacggtg
     2521 gcccaggcgt cgagcagggc cagggtcggg tcgtcttcgg cgcgggtctg caactcgtcc
     2581 agggccggtt ggctggagag cgcggcctgc atggtctcct tgaagct
//
LOCUS       DASUYC010000706         2741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_801313,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000706 DASUYC010000000
VERSION     DASUYC010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801313"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1209)
                     /locus_tag="VF276_11545"
     CDS             complement(<1..1209)
                     /locus_tag="VF276_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607536.1"
                     /translation="MQDLTRFRFRTVLTGSTAITVTEGAEFGTSMLIMTETVRTGKHQ
                     TQHQEVEIGGSSWENFGGGWIEQPYVSAEERLFTLTLADIVRFSLDSIEVADDLGDTT
                     LDGVGARHLRITTNADSIGRVATGEVWIDRATSYVAQIQLEPVVAQEAGLQGIAIKMW
                     QFNDPPSTIEGPLIDARAITPIGLPPRGQRSDLNLNGISMVSPGEGWAVGRQGPQPVL
                     LHYTRGRWILTPAPTYLWLAGVCMTAATDGWAVGEDMRILHYQRGQWTRVDSPAAKTH
                     PESALISVTMTSPHDGWAVGMDGLILHYARGAWTEVPSPTKAALYGVSMVSPDEGWAV
                     GQESSMDVSGVVLHYHAGKWTQEPKISRDSLRGISMISSDEGWAVGNQGTILHYIHGQ
                     WLHQDNVAGGD"
     gene            complement(1827..>2741)
                     /locus_tag="VF276_11550"
     CDS             complement(1827..>2741)
                     /locus_tag="VF276_11550"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HEX6607537.1"
                     /translation="DRDNKPGIAFALNNLAMQLREQGGQERALALCQESLVLYRALGD
                     RRGTAMLLHNLGDLVQEQGDHTRAEQLFQESVALEQALGDNWLVARTTTNLGYLALAQ
                     GRPATAATAFTEALATSQQLEDKLGLAQVLMGFGQLASRQGQPERSVRLFAAADALLH
                     AAGARLVGGGTAEINHDIDAARIQLDKAAWEAAWAAGQTMPLEDVIACALQVPTAAEP
                     AHEPVQSSLSSPAPTASTTRRDPDALTAREVEVLRLVAAGLTDAEIATRLVLSPRTVQ
                     VHLRSIYSKLGLTTRSAATRYAIGHHLA"
BASE COUNT          477 a          852 c          873 g          539 t
ORIGIN      
        1 gtccccgccc gccacgttat cctggtgcag ccattggcca tgtatgtaat gcagtatggt
       61 gccctgattg ccgaccgccc aaccctcatc tgacgagatc atgctgatac cgcgcaggga
      121 gtccctagag attttaggct cctgcgtcca ttttcccgca tgatagtgca gcacaacacc
      181 gctgacatcc attgaagatt cttgtccaac agcccaaccc tcgtccggcg aaaccatgct
      241 gacgccgtag agcgcggcct tggtgggact cggcacctcg gtccatgcgc cccgggcgta
      301 gtgcaggatg agaccgtcca tcccgacggc ccaaccgtcg tggggcgacg tcatggtcac
      361 ggagatcaag gcgctctcgg gatgggtttt cgcggccgga ctatcgaccc gagtccactg
      421 accgcgctgg tagtgtaaaa tccgcatatc ctcgcccacg gcccagccat cggttgccgc
      481 ggtcatacac acgccggcta gccacagata agtgggggcc ggtgtcagaa tccagcgccc
      541 tcgcgtgtaa tggaggagca ccggctgcgg cccctgtcgg cccacggccc agccttcgcc
      601 cggcgatacc atactgatgc cgttcagatt caggtcgctt cgttgcccgc gggggggcag
      661 cccgatcggc gtaatcgctc gggcatcaat gaggggtcct tcgatggtgc ttggaggatc
      721 attgaactgc cacattttga tggctatgcc ctgcaggccg gcttcctgtg cgaccaccgg
      781 ctcaagctgg atttgggcca cataactggt ggcgcggtca atccacacct ctccagttgc
      841 caccctgccg atcgaatcgg cgttcgttgt tatgcgtaga tgacgagcac ccactccgtc
      901 gagagttgta tcgcccagat cgtcggccac ttcgatcgaa tcaagactga aacgaacgat
      961 atcggccaaa gtcaaggtaa ataagcgttc ctcagcggag acgtagggct gctcgatcca
     1021 tcccccgccg aagttttccc agctcgaccc cccgatctcg acctcttggt gctgggtttg
     1081 atgcttaccc gtgcgcaccg tttcggtcat aattaacatg ctggtcccaa actccgcacc
     1141 ttcggtaaca gtgatggcgg ttgagccggt caggacggtg cgaaagcgaa atcgggtgag
     1201 atcctgcatt gcctcatctg ctttccgaat aaagggtgcc ccgctgccgg tgggacctgg
     1261 agtgggggtg ggcgtgctct cttgcgtgcg gggcacgagg gtcggcggcg ccatcggttg
     1321 ggccggcgcc gttgggctag gggggagcgt gcgggtgggc ggcgcggatg ggctcgttac
     1381 tcgcgtagcg gtgcggtctg ggcgcatggc cggggtgggc gaagcggcgg gcgtgggcgt
     1441 cgcgttctcg ccgcaggccg tagtcaagcc caataggcac agcagaacca atagcgggaa
     1501 ccagtacact gtcttcatcg acggacctcg ctttcgcgca cggtgaccag ggccaacctg
     1561 ctcctgggct agcgatgcgt ctgatcgcac tatagcaccg ttcctacctg ccgccatgcg
     1621 cagtcctgct taacttttcg gcccggttgg aaaaggctcg ctgaaccgca taagtaaagc
     1681 tgcttacgcg gggtgagcag gtgaatgggg acagacgcaa ggcgtggaca tgccaatgcc
     1741 gaggtgaaac acgatagata cagtgatcct ctcgtgagtg gtcccgtgca agagcgacag
     1801 tatcgaacct accggcgaaa gtgacctcag gctaggtgat gtccgatggc gtagcgggtg
     1861 gcggcgctgc gggtggtgag gccgagtttg ctgtaaatgg agcgcagatg cacctgcacc
     1921 gtgcgcgggc tgagcaccag gcgcgtggcg atctcggcat ccgtcagccc cgccgccacc
     1981 agccgtagca cctccacctc ccgcgccgtc agggcgtccg ggtcgcgccg ggtggtgctc
     2041 gctgtggggg cggggctgga taggcttgat tgcaccggct catgggccgg ttcggcggcg
     2101 gtggggactt gtaacgcgca ggcgataacg tcctccaacg gcatcgtctg gcccgcggcc
     2161 cacgctgctt cccaggccgc cttgtccaac tgaatacggg ctgcatcaat atcgtggtta
     2221 atttcagctg tgcctccccc aacaaggcgt gcgccagcgg catgcaggag ggcgtcggcc
     2281 gccgcaaaca gccgaactga tcgctctggc tggccctgcc tgctcgccaa ctggccgaat
     2341 cccatcaaga cctgtgctag cccaagcttg tcttcaagtt gctgcgatgt cgctaaggcc
     2401 tcggtaaaag cggtcgctgc tgtggcgggg cgaccttgcg cgagcgccag atagccgagg
     2461 ttcgtggttg tgcgggccac cagccagttg tcgccgagcg cctgctccag tgcgacgctt
     2521 tcctgaaaga gttgctccgc gcgcgtgtgg tccccctgtt cctggaccag gtcgcccagg
     2581 ttatgcagca gcatggctgt cccgcgccgg tcgcccaatg cccggtacaa caccaggctt
     2641 tcctggcaca gggccagggc tcgctcctgg cctccttgct cgcgcaactg catcgccagg
     2701 ttgttgagcg caaaggcgat accgggcttg ttgtcgcggt c
//
LOCUS       DASUYC010000707         3034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1955950, whole genome shotgun sequence.
ACCESSION   DASUYC010000707 DASUYC010000000
VERSION     DASUYC010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1955950"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VF276_11555"
     CDS             complement(<1..445)
                     /locus_tag="VF276_11555"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX6607538.1"
                     /translation="MTRVRIALAQIRCEKGDWPGNLDRAAGYMAQAAAQGGDIIVFPE
                     AGLSGYCDPARFPQAVQPLDSPLVRQFVDQAARAGIAASGGFLETNPGGKPFVTQILA
                     QDGQIAGVYRKVNLGEDEPFYTAGSAMPVFTLHVRDGAVPCALAIC"
     gene            826..2754
                     /locus_tag="VF276_11560"
     CDS             826..2754
                     /locus_tag="VF276_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607539.1"
                     /translation="MRLSRVVRLLSGVALAALLITQGGRPPVAPLPAEAAAGSASPFG
                     MNLYITGLERDDRERNVLIDRAREAGVAWSREELSWANIQPSERGEGWKPYDTRLQQL
                     ANAGISVIGVLLTTPDWASGHSKSENGWYWYPPRDPHTYGAFVEAAVRRWHDKVAVWE
                     LWNEPDKDGTWLPHADPAAYAALLAEGYAAVKRADPTAKVMVGSVYVHDSGNEGLAFM
                     EQVVAASGGRLNFDLMGIHTFMNDRQPEDTSPVTVVQNLPWRLQVTRQWLTRHGRGDM
                     PIWITEDGQSVCQGCGNQGTDEQRQAARLVRRYVLALANGAAHFDYFQMKDKFNSGLS
                     DVWGNMSVLRNDLSPRPAFYAYKTMTALLGAGQLAGPGQLQHAVANRWQPQFDRYDYR
                     FSTPGGTVHVLWVPENVAPQTSQLPVSTPGARIVQMDGSAQTVPAQNGHVTVQITSNP
                     IFVVEEVPAPAGGIDPATDPQSPTGFRVSTRFADFWRSHGGLAAFGYPITGERQEVSP
                     TNGKSYIVQWFERTRFEWHPENQPPFNVLLGLMGRQVTAGRGFPTVAPFANTADRRYV
                     PETGHSLSGRFLQYWSTTGGIALYGYPISEEMREVRPTDGKTYTVQYFERARFEYHPE
                     NAPPYDVLLGLLGRQLFR"
BASE COUNT          511 a         1081 c         1000 g          442 t
ORIGIN      
        1 cgcagatcgc cagggcacag ggcacggcgc cgtcgcgcac gtgcagcgtg aaaaccggca
       61 tggcgctgcc cgccgtgtag aatggctcgt cctcgccgag attcaccttg cggtacactc
      121 cggcaatctg cccatcctgg gccaggatct gcgtgacgaa gggcttcccg ccgggattgg
      181 tctccaggaa cccgcccgac gcggcgatgc cggcgcgcgc cgcctggtcc acgaactgcc
      241 gcaccagcgg cgagtcgagc ggctgcaccg cctgcggaaa gcgcgccgga tcgcaatagc
      301 ccgacagccc cgcctccgga aagacgatga tgtcgccgcc ctgcgcggcg gcctgggcca
      361 tgtagcctgc cgcccggtcg agattgccgg gccagtcccc cttctcgcag cggatctgcg
      421 ccagcgcaat gcgtacacga gtcatgagcg caaccattct ccttccaggc accaccggcg
      481 actgatgcgc gccggtcagg tccagtatag atgatcgggg ctacacctca aagccgggcg
      541 ccccggcgcg gcgagatcgc ccggcccgtt aacgggcggg cttcgttggc gtagcctgcg
      601 tgcgcgggct gggccaatcg agagaggccg cgcaggcggc cttcgtcgcg ctagccggag
      661 gcttgagcct ccgggcggcg ccaccattgt cgcaaccttg acatcttgtc gcggcgctgg
      721 ctataattag ccctgtatgt agcctggcct gccgtctatt gatgcccgcc catcccctgg
      781 gcgggccgcg aaatccatcg cagtcgcgct ctagaaggac cctgtatgcg cctgtcgcgc
      841 gttgtacgcc tgttgtccgg ggtggcactc gccgccctgc tgattaccca ggggggccgg
      901 cctccggtcg cgcccctgcc cgccgaggcc gccgcgggat ccgcgtcgcc cttcggcatg
      961 aacctctata tcaccggcct ggagcgcgac gaccgcgagc gcaacgtgct gattgaccgc
     1021 gcccgcgaag ccggcgtggc ctggagccgc gaggagctaa gctgggccaa catccagccc
     1081 agcgagcgcg gtgagggctg gaagccctac gatacgcgcc tgcaacaact cgccaacgcg
     1141 ggcatctcgg tcatcggcgt gctgttgacc acgcccgact gggcctccgg gcacagcaag
     1201 agcgagaacg gctggtactg gtacccgccg cgcgacccgc acacctatgg tgccttcgtc
     1261 gaggccgccg tgcgccgctg gcacgacaag gtggccgtct gggaactgtg gaacgagccc
     1321 gacaaggacg gcacctggct gccgcacgcc gatcccgccg cctacgccgc gctgctggcc
     1381 gagggctacg cggcggtcaa gcgcgccgac ccgacggcca aagtgatggt cggctcggtc
     1441 tatgtccacg acagcggcaa cgagggcctg gcgttcatgg agcaggtcgt ggcggccagc
     1501 gggggccggc tcaacttcga cctcatgggc atccacacct tcatgaacga ccgccagccg
     1561 gaggacacgt cgccggtcac ggtggtgcag aacctgccct ggcggctgca agtgacccgc
     1621 cagtggctga cccgccacgg gcgcggcgac atgccgatct ggatcaccga ggacgggcag
     1681 agcgtgtgcc agggctgcgg caaccagggc accgacgagc agcgccaggc cgcgcggctg
     1741 gtgcgccgct atgtgctggc cctggcgaac ggcgccgccc acttcgacta tttccagatg
     1801 aaggataagt tcaacagcgg gctgtcggac gtctggggca acatgagcgt gctgcgcaac
     1861 gacctcagcc cgcgaccggc cttctacgcc tacaagacga tgaccgcgct gctcggcgcg
     1921 gggcaactgg ccgggcccgg ccagctccag cacgccgtgg cgaaccgctg gcagccgcag
     1981 ttcgaccgct atgactaccg cttcagcaca ccgggcggca cggtgcatgt gctgtgggtg
     2041 ccggagaatg tggcgcccca gacgtcgcag ttgccggtca gcacgccggg cgcgcgcatc
     2101 gtgcagatgg acggcagcgc ccagacggtc ccggcgcaga acgggcacgt gactgtgcag
     2161 atcacgtcca acccgatctt tgtcgtcgag gaggtgcccg cgccggcggg aggcatcgac
     2221 ccggccaccg acccgcagtc gcccactggc ttccgcgtca gcacgcgctt cgcggacttc
     2281 tggcgcagcc acggcgggct ggcggcgttc ggctacccga tcaccggcga gcggcaggag
     2341 gtcagcccca ccaacggcaa gtcgtacatc gtgcagtggt tcgagcgcac ccgcttcgag
     2401 tggcacccgg agaaccagcc gccgttcaac gtgctgctcg gccttatggg ccgccaggtg
     2461 acggcggggc gcggcttccc gaccgtggcc cccttcgcca acaccgccga ccggcgctac
     2521 gtgcccgaaa ccggccacag tctgagcggg cgcttcctgc aatactggtc cacgaccggc
     2581 ggcatcgcgc tctacggcta ccccatcagc gaggaaatgc gcgaggtcag gcccacggac
     2641 ggcaagacgt acacggtgca atacttcgag cgcgcccgct tcgagtacca ccccgaaaac
     2701 gccccgccct acgacgtgct gctcggcctc ctgggccgcc agcttttccg gtaagcaaat
     2761 atcccgatgc gccggcgggc gcaatgaatt gcggctgacg tcacgcggcg acgggcgcaa
     2821 taaattgcgc cactacgaat gatgctacgt agtggcgcaa tttattgcgc ccgtcgccca
     2881 accccgatct ccgatgccag agatcaaccg cccgccgcca cgccccaggc accaactgtc
     2941 attctgagcg cagcgaagaa tctcgtctcc tgtccgacca aggtgagact ctgcgctacg
     3001 cgcagagtga cacgagggag acgagactct gcgc
//
LOCUS       DASUYC010000708         2793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1262144, whole genome shotgun sequence.
ACCESSION   DASUYC010000708 DASUYC010000000
VERSION     DASUYC010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262144"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..736)
                     /locus_tag="VF276_11565"
     CDS             complement(137..736)
                     /locus_tag="VF276_11565"
                     /inference="COORDINATES: protein motif:HMM:NF021081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2378 family protein"
                     /protein_id="HEX6607540.1"
                     /translation="MANARPPAPNPTIRITRPPVPDAEKRYLTQLDVALETAGDRLTP
                     ALRAQIEQEFGAYLGQTGHPCLVANRLDDLLCRACFADHLMPEARRLFGRASVGHYRE
                     SILGRVMLAAIPIMGMERIVRLIPRSFAATTNYGTRVATEIGPRHWRCEFEDEIMYPE
                     IIQGVLEGIGELARVPGLQITCTPRGPAHFAFDITWQAR"
     gene            complement(958..1938)
                     /locus_tag="VF276_11570"
     CDS             complement(958..1938)
                     /locus_tag="VF276_11570"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6607541.1"
                     /translation="MTQVKEPAAPHHVEDEFVFRPFDMDRDVLPLLRLINEIEAIDQV
                     DEGRSESELRASLTWPEGDPARDRVVVAEPGRPERLIAAVTTWKASTTDRADVRIMVH
                     PDWRRRGLGRALLARGLARAREKGAVYLSAGHDNRMRAAGAFLHAHGFRPTATWIQMR
                     CAAEVPLAEPVYPAGYTVRPQSEVQSVPVVAEALNRGFIGHFEHRDATDTEVAHWLGT
                     DHTRPDGIFLAFDPAGEPAGICWAEINPDHSARRGRPTGYIDSLAVVPEHRRHGLGRA
                     LLLEGMRWLRANGQATIELDAWGENERALPLYEGAGFRISRQGQSYERTL"
     gene            complement(1935..>2793)
                     /locus_tag="VF276_11575"
     CDS             complement(1935..>2793)
                     /locus_tag="VF276_11575"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6607542.1"
                     /translation="TSEAIVRSRLQQPGHDPAHDRWVVAEPGNPDHLIGHAMVARSPE
                     APFAWINTQVHPLWRRRGLGRALLARALDRARALGASYATTGTAAHLTGAQAFLTGQG
                     FRPRNTFVEMQILPEVPLAEPVYPPGYTVRTYADVNDLPTLMTAYNRGFIGHFEHHDE
                     TAEELAHWLALPHIRPEGFFLAFGPAGDPAGICAALINPDRSAARGRPTGYIDSLGVV
                     PEHRRHGLGRALLLEGMRWLRANGQEVIELNAVGDNEMALPLYEGVGFSVVHRNIGYR
                     YDFDGEQTP"
BASE COUNT          404 a          927 c         1035 g          427 t
ORIGIN      
        1 gtggcggcgc tattgatcgc gcccgtcagg gcgtggcaag gctacccgga ggcgggcgag
       61 cgcttcctga gaccacggcg gacacgattg tgtccagatc aaatctgcgg gcggtccagg
      121 tggcgggcgt cccgccttac cgggcctgcc aggtgatatc gaacgcgaaa tgcgcgggac
      181 cacgcggagt gcaggtgatt tgcaagccgg gcacgcgggc cagttcgccg ataccctcca
      241 gcacgccctg aatgatctcc gggtacatga tctcgtcctc gaactcgcag cgccagtggc
      301 ggggaccgat ctcggtcgcc acacgggtgc cgtaattggt tgtcgcggcg aagctacgcg
      361 ggatcaggcg gacgatgcgc tccatgccca tgatcgggat cgccgcgagc atcacccggc
      421 ccaggatcga ctcgcgatag tggcccacgg aggcccgccc gaagagccgg cgggcttccg
      481 gcatgaggtg gtcggcgaaa cacgcgcggc acaacaggtc gtccagccgg ttggcgacca
      541 ggcaggggtg cccggtctgg cccaggtagg cgccgaactc ctgctcgatc tgcgcgcgca
      601 gggccggcgt gagccggtcg ccggcggttt cgagcgccac atccaactgg gtgaggtacc
      661 gcttctcggc gtcgggcacc ggcggtcgcg tgatgcgaat ggtgggattg ggggcagggg
      721 ggcgggcgtt ggccattggc ggttccctcc gcgatcgcac gcgcccgatc tacggttggc
      781 gaatcggttg cagatcagcg cgtggcttac gattcatgat agcacagccc gcgacccggc
      841 gcaaccgccg ggcaaagcgc acggcgcgct catgccggtt cctgcggtcc acggatgaaa
      901 ttgtaggggc cggttggcaa ccggccccta cggaatgcga ttcacgatgc gccgcgccta
      961 caacgtgcgc tcgtaggact ggccctggcg gctgatgcgg aagccggcgc cttcgtagag
     1021 cggcaaggcg cgctcgttct cgccccaggc atcgagctcg atggtcgcct ggccgttggc
     1081 gcgcagccag cgcatcccct ccagcagcag ggcgcggccc aggccgtggc gccggtgttc
     1141 cggcaccacg gccaggctgt cgatatagcc cgtgggccgg ccccgccggg cgctgtgatc
     1201 cgggttgatc tcggcccagc agatgcccgc cggctcaccc gccgggtcga aggccaggaa
     1261 gatgccgtcg gggcgggtgt ggtcggtgcc cagccagtgg gccacttcgg tgtcggtcgc
     1321 gtcacggtgc tcgaagtggc cgatgaaacc gcggttcagc gcctcggcca cgacagggac
     1381 gctctgcacc tcgctctgcg gccgcaccgt gtagcccgcg ggatagaccg gctcggccag
     1441 gggcacctcg gcggcgcagc gcatctggat ccaggtggcg gtcggacgga agccgtgcgc
     1501 gtgcaggaag gccccggcgg cgcgcatgcg gttgtcgtgc cccgcgctca ggtagacggc
     1561 gcccttctcg cgggcgcggg ccagaccgcg cgccagcagg gcgcggccca ggccccgccg
     1621 ccgccagtcg gggtgcacca tgatacgcac gtcggcgcgg tcggtggtcg aggccttcca
     1681 ggtggtcacc gcagcgatca gccgctcggg acggcccggc tccgcgacca ccacgcggtc
     1741 gcgcgccggg tcgccctccg gccaggtcag gctcgcgcgc agctcgcttt ccgagcggcc
     1801 ttcgtccacc tggtcgatgg cttcgatttc gttgatcagc cgcagcaggg gcagtacgtc
     1861 acggtccatg tcgaaggggc ggaagacgaa ttcgtcttcg acgtggtgcg gcgccgcggg
     1921 ttcttttact tgagtcatgg agtttgctcc ccgtcgaagt cgtagcggta gccgatgttg
     1981 cgatggacga cgctgaagcc gacgccctca tagagcggca gggccatctc attatcgccc
     2041 acggcgttca attcgatcac ttcctggccg ttggcgcgca gccagcgcat accttctaac
     2101 agcagggcgc ggcccaggcc gtggcgccgg tgttcgggca cgacgcccag gctgtcgata
     2161 tagccggtgg gccggccacg ggccgcactg cggtcgggat tgatgagggc cgcgcagatg
     2221 cccgcggggt cgcccgccgg gccgaaggcc aggaagaagc cctccgggcg aatgtgcggc
     2281 agggccagcc agtgggccag ttcctccgcg gtctcgtcat ggtgctcgaa gtggccgatg
     2341 aagccgcggt tgtacgcggt catcagcgtg ggcaggtcgt tcacgtcggc gtaggtgcgg
     2401 acggtgtagc cgggcgggta gacgggctcg gccagcggca cctccggcaa gatctgcatc
     2461 tcgacaaagg tgttgcgcgg gcggaacccc tgcccggtca ggaacgcctg cgcgccggtg
     2521 agatgcgcgg cggtgccggt ggtggcatag ctcgcaccca gcgcgcgggc gcgatccagg
     2581 gcgcgcgcca gcagggcgcg gcccaggccc cgccgccgcc acagcggatg cacctgcgta
     2641 ttgatccagg caaacggcgc ttccggcgac cgggcgacca tcgcgtggcc gatcaggtgg
     2701 tcgggattgc cgggctcggc caccacccag cggtcgtggg ccgggtcgtg gccgggctgc
     2761 tggaggcgcg accgcactat ggcctcgctg gtg
//
LOCUS       DASUYC010000709         2915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_569430,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000709 DASUYC010000000
VERSION     DASUYC010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569430"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="VF276_11580"
     CDS             complement(<1..801)
                     /locus_tag="VF276_11580"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX6607543.1"
                     /translation="MNACEETAVLPAAPAGSRGGIPIGGTVLGPDGGPAIGIVDKVVL
                     SPLTGEVTHLLIRCFGRWRYDVLVPMTGVGAVDRGVVRLRVPVAQLNRPAARDPGNTA
                     APPEPWAGPHGYAPDQVLFALSERVCLPDDRTDDDIAAQAVAALRSYGSTRQLLRDLE
                     SAATVTANLVPDGTIRIAVRDSVVILEGNVELHSYAGMVERLVRRVPGVREVLNLLIA
                     DDDLQTSVTAALHYDPRTRHLQPGIGVAQGQVTLTGSVADAGSRAAVEA"
     gene            1243..1464
                     /locus_tag="VF276_11585"
     CDS             1243..1464
                     /locus_tag="VF276_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607544.1"
                     /translation="MRTNYAQPRNSADLAALVHMRVSAFHPHGQAVVLMSPWPFPKVL
                     RRQEARRWMSPMMDAGTARRQGGSLPVAV"
     gene            complement(2145..2696)
                     /locus_tag="VF276_11590"
     CDS             complement(2145..2696)
                     /locus_tag="VF276_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607545.1"
                     /translation="MRYLEVRRHTMRTKPGEHLSQAGVDLARRVGTTMGPFADVVTTR
                     RPWAFETAIAMGFAVDSQLAAFPPMPADGAGAAAWDAGYAAWCRAVRQDGVAARYAHQ
                     EAACWRAIVAAVPDGASALLVTHGGVIEAGAAACLSREEHTRLAAGCSYCEGIRLAWD
                     GTGWAGLEVLPVGPARPEAPLVR"
BASE COUNT          468 a         1001 c          980 g          466 t
ORIGIN      
        1 ggcttccacg gcggcccggc tgccggcatc ggccacgcta cccgtaagcg tcacctggcc
       61 ttgggcgacc cctatgccgg gctgtaaatg gcgggtgcgc ggatcgtaat ggagggccgc
      121 cgtgaccgag gtttgcagat cgtcgtcggc aatcagcagg ttcagcacct cgcggacgcc
      181 ggggacccgc cgcaccagcc gctccaccat cccggcatag ctgtgtagct ccacgttgcc
      241 ctccaggatg acgacgctgt cgcgcaccgc aatgcgtatc gttccatcgg ggaccaggtt
      301 agccgttacc gtcgcggccg actcgagatc ccgtaacaac tggcgagtgg acccatagga
      361 tcggagggcc gcgaccgcct gcgccgcgat atcgtcgtcg gtcctgtcgt cagggaggca
      421 cacgcgttcc gatagcgcga agagcacctg atccggtgcg tagccgtgcg gcccggccca
      481 gggttcaggc ggggcggcgg tgttgcccgg atcgcgggcg gccgggcggt tcaattgtgc
      541 gacgggcacc ctgagccgca ccacgccccg atcgaccgca cccactccgg tcataggcac
      601 caggacatcg tagcgccatc ttccaaagca cctgatcagc aggtgcgtta cctcgcctgt
      661 tagtggggaa aggacaactt tgtcgacgat gccaatcgcc ggtccaccgt ccggccccag
      721 cacggtgccg ccgatgggaa tgccgccccg actcccggcg ggagccgcgg ggagcacagc
      781 cgtctcttcg caagcattca tctcatttgc tccttcggtt tactcgaagg cccaagaccg
      841 ggataggtag cgattctgct tcgctggcga ggccgcgacc cgtgttactt gcgcgctcgg
      901 ccctgggccg gccaccgcgc gcaccgccac ccggacgcgc catacgcaaa cgtatcaccg
      961 atgcgcccac tgcggcagcg catcggaacc cgactgctgg tcgcggtgca cctctctatt
     1021 tgaattctac ccccgctctt tgaaggttaa gcgaagaggg ggttaaggtt ctgttaagat
     1081 tggcccggag caacggctat tccggcatgg ccgggcggac gccggcgcac cggctgatcg
     1141 gggagctttt gatccggctg ctgcgcagtc ctattgaggg ccggtgggcg agatcggtca
     1201 tgcggcatcc cgcgcggccc ggacgcggaa gctatagagc ggatgcgcac caattacgcc
     1261 cagcccagga acagcgcgga cctggccgcg ttggtgcata tgcgggtgtc cgcgttccac
     1321 ccgcacggcc aggcggtcgt gttgatgagt ccctggccgt tcccgaaggt gctgcggcgg
     1381 caggaggcgc ggcggtggat gtcgcccatg atggatgccg ggacggcgcg ccggcagggg
     1441 ggatctctac ctgtcgccgt ttagtcgcga atacgatagc ttcttcgagc aggcgccaag
     1501 ccgggacggc gcccggcggc gggaccaggg cgttgcagcc gccgccgctg ctgcgtaacc
     1561 ggatcaggca catggcgccg cagcctgaca tccccagatg tttccaccac cgccagccta
     1621 tgaggcgata agcccaccgg ccattactga aacagctctg gcggcaggag acctgcgccc
     1681 agaggcgggg gcacgcgatg gccgatgagt cagcgacagc cacgcctcaa gcgattgaga
     1741 attctgggct aatggccagt cgcggcagtc ccggttgcgc cttgccaaaa ccctccgtta
     1801 tgcggacgtt gattctggaa cggtttttgg aacaggtgct gagggtgaac aggcacaaag
     1861 ccaacctaca actttgtccg acatttagct ctgaatgggc cgagcgtgca atttcacagc
     1921 cagcctatga aaagcggggt cagcgagagt gccagaatcg gacgttattg gagaccattg
     1981 caaaccggga gctaggcgtg aagccgctcc caattgggct ttggcctggt ctcggctgtc
     2041 cactcgggcc acccaacttc ccgctccggt tggcccgggt tgccttgttc ggccccctga
     2101 ctactctcca cagtcccgtc ccacccgcct gggccggacc ttcgtcagcg caccagcggc
     2161 gcttcgggcc gggccgggcc caccggcagg acttcgaggc cggcccaccc ggtgccgtcc
     2221 cacgccaggc ggatgccctc acaataggag cagcccgccg cgaggcgggt atgctcttcg
     2281 cgggacaggc aggcggcggc ccccgcttcg atgacgccgc catgggtgac cagcaaggcg
     2341 ctggcaccat cgggcacagc ggcgacgatc gcgcgccagc acgccgcctc ctggtgggca
     2401 tagcgggccg ccaccccgtc ctggcgaact gcccggcacc aggcggcgta cccggcgtcc
     2461 caggccgctg cgcctgcccc gtccgcgggc atcggcggga aggcggctag ttggctgtcc
     2521 accgcgaagc ccatggcgat ggccgtttca aaggcccagg gccggcgggt ggtgaccacg
     2581 tcggcgaacg gccccatcgt cgtgcccacg cgccgggcca ggtccacgcc cgcttgcgag
     2641 aggtgctcgc cgggcttggt ccgcatggta tggcggcgca cttccaggta gcgcatcaag
     2701 tggggggtct cctttcaccc ggaatccggc gggatcccgg cccagcgccg gtcggccggc
     2761 ataaagcggg gcggcccggc cagccgccct gctcccggcc gggaggcatt cgtgatggtc
     2821 gcgcaccgcc cgcgccggaa cgccggatcc ttccggcgcg ggcggtgcgc tcctgggcta
     2881 gcggcggacg cgcacgttgc gccgccgcag cccca
//
LOCUS       DASUYC010000710         2130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_339409,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000710 DASUYC010000000
VERSION     DASUYC010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339409"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VF276_11595"
     CDS             <1..588
                     /locus_tag="VF276_11595"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX6607546.1"
                     /translation="IDEIQDRFINEWGDLVTVWGLNKTMGQIHALLYITGQPLTADEI
                     MERLNISRGNVSMNLRALMSWGVVHRQHRKGDRKQYFVAEEDPWRWFKNVVRERRKRE
                     VEPIIEAFSDTLSLLPPSHPSPNGKAQPTEAAGEDTQELRARLEQMRDFVQVFNRGIE
                     MFLRFSREDFNELMELLAATQEPGSEIARRALASK"
     gene            593..1411
                     /locus_tag="VF276_11600"
     CDS             593..1411
                     /locus_tag="VF276_11600"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6607547.1"
                     /translation="MAAPTVQVTTIPVQGRPCHYWSAGSTAAPPLVLLHGGLGNAALH
                     WGRNLAGLGRDFHVLAPDLPGFGRTAPLPRPGYRAYAAWVAAFCDAVGIPAGITVAGN
                     SMGAAIARVFAATYPDRVARLVLVDGGRPVAVGPPLRQILAIGPLGDLLAAVLGRLAG
                     LDAMMSRYVANPALLTPRVRAGIRRGIRAYVRVQRRILLEPPVPAAALAVQCPTLVIW
                     GAQDGLGSPALGEALAAQTGAGPVAVIDAAGHMPMFEQPAAFARVLRAFLAAPA"
     gene            1868..>2130
                     /locus_tag="VF276_11605"
     CDS             1868..>2130
                     /locus_tag="VF276_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607548.1"
                     /translation="MFTDSESISFVSPVDAGPASDLVEGMVIDGNRGQYRVETPDGVV
                     LCTLRGKLRKDLIHPISHNVLQKARRVNTKARAPVAVGDRVRVL"
BASE COUNT          313 a          779 c          697 g          341 t
ORIGIN      
        1 atcgacgaaa tccaggaccg gtttatcaac gagtggggcg acctggtgac ggtctggggc
       61 ctgaacaaga ctatgggcca gatccacgcc ctgctctaca tcaccggcca gccgctgacc
      121 gccgacgaga tcatggagcg cctcaacatc agccggggca acgtgagcat gaacctgcgc
      181 gcgctaatga gttggggcgt ggtccaccgc cagcaccgca agggcgaccg caagcagtat
      241 ttcgtggccg aggaagaccc ctggcgctgg ttcaagaacg tggtgcgcga gcggcgcaag
      301 cgcgaggtcg agccgatcat cgaagccttc agcgacacgc tgtcgctgct gcccccgtcc
      361 catcccagcc ccaacggcaa ggcgcagccc accgaagcgg cgggcgagga cacgcaggaa
      421 ttgcgcgccc ggctggagca aatgcgcgac tttgtgcagg tcttcaaccg cggcatcgag
      481 atgttcctgc gcttcagccg cgaggacttc aacgagttga tggagctgct ggccgccacc
      541 caggagccgg gcagcgagat cgcgcgccgc gccctcgcca gcaagtagcg ccttggctgc
      601 gcccaccgtt caggtcacga ccatcccggt acagggacgc ccctgtcact actggagcgc
      661 cggttcgacc gctgcgccgc ccctggtgct gctgcacggc ggcctgggca acgccgcgct
      721 ccattggggc cgcaacctcg ccggcctggg ccgcgatttc cacgtcctgg cgcctgatct
      781 gcccggcttc ggtcgcaccg cgcccctgcc ccgccccggc taccgggcct acgccgcctg
      841 ggtcgccgct ttctgcgacg ccgtcggcat cccagcgggc atcaccgtcg ccggcaactc
      901 gatgggcgca gccatcgccc gcgtgttcgc cgcgacctac ccggaccgcg tcgcccgcct
      961 cgtgctggtg gacggcgggc gcccggtcgc cgtcgggccg cccctccgcc agatcctcgc
     1021 catcggcccg ctgggcgatc tgctcgccgc cgtgctgggg cggttggccg gcctggatgc
     1081 catgatgagc cgctatgtcg ccaacccggc cctgctcacg ccacgggtgc gcgcgggcat
     1141 ccgccgcggc atccgcgcct atgtccgcgt ccagcggcgc atcctgctgg agccgccggt
     1201 gcccgccgcc gcgctggccg tgcagtgtcc cacgctggtg atctggggcg cgcaagacgg
     1261 cctgggatcg cccgcgctcg gtgaggccct ggccgcgcag acgggcgccg ggccggtcgc
     1321 cgtgatcgac gccgccgggc acatgcccat gttcgagcag cccgccgcgt tcgcccgcgt
     1381 gttgcgcgcg ttcctcgccg cgcccgcgta agtcgctcgg cccgtgaacg agcgagtacg
     1441 gcggcctcgc ccggctccta aaggagcggg ctaccgcgac gaaggccgcc tgcgcggcct
     1501 gtcttgggct accctgcgtg ttgtcccggt tccgttccgc cttgcgatgt attgacaccg
     1561 caccgtccgc cctctatact ggtcctcaac agcggctcgc ctgagcggct gccccgagat
     1621 gtcaagactt gcgcggcctg tgcggtcgcg cccagagcga aaggaggcgt tgctgatgaa
     1681 aggcatccat ctgctgtatg cagcgccgcc gcgtctggtc ctccatcggg gaggggccta
     1741 gcgcccccgc gcagacggct ccggtcgtcc ttcgctgcat gcagaaccgg cgggcctacc
     1801 cgaccggctt tttgtttgtc catcacaacg taactgcatg catagcggcg aaaaggagcc
     1861 tattgctttg ttcactgatt ccgaatctat ctccttcgta agccccgtgg atgccgggcc
     1921 ggcgtccgat cttgtcgagg gcatggtcat cgacggcaac cgcgggcaat accgtgtcga
     1981 aaccccggac ggcgtggtac tctgcaccct gcgcggcaag ctacgcaaag acctgatcca
     2041 ccccatcagc cacaatgtgt tgcagaaggc gcggcgcgtc aacaccaagg cccgcgcgcc
     2101 ggtggccgtg ggcgaccgcg tgcgcgtgct
//
LOCUS       DASUYC010000711         2608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1032122, whole genome shotgun sequence.
ACCESSION   DASUYC010000711 DASUYC010000000
VERSION     DASUYC010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1032122"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2608)
                     /gene="treS"
                     /locus_tag="VF276_11610"
     CDS             complement(<1..>2608)
                     /gene="treS"
                     /locus_tag="VF276_11610"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HEX6607549.1"
                     /translation="VSPLRDDGYDIADYTAVHPLYGTLGDFKRFLRAAHDRGLRVITE
                     LVLNHTSEEHPWFQRARQAPPGSNWRRFYVWSDTPERYPAARVIFKDFEASNWSWDPV
                     AEAYYWHRFYAHQPDLNFDHPPVRKAMLQVVDFWLKLGVDGLRLDAVPYLYEREGTTG
                     ENLSETHAFLQELRAHIDRHYPDRMLLAEANQWPEDAVAYFGTGNECHMAFHFPLMPR
                     LFMSIRREERWPIIDIMAQTPAIPDTAQWALFLRNHDELTLEMVTDEERIYLYRAYAQ
                     DPAARVNLGIRRRLAPLLRNDRRRIELLNALLFSLPGTPVIYYGDEIGMGDNIYLGDR
                     NGVRTPMQWSADRNGGFSQANPQQLYLPLIVDHEYHHESVQVESQQSNPHSLLAWMRR
                     LIAMRKRYRAFGRGTLEFLHPENRKVLVFLRAYEDEQILVVANLSRFAQHVELDLAAF
                     QGSVPVELFGRVPFPPIGELPYLLTLGPHSFYWFALEPAPVPAAPAEQAALPALAAPD
                     GWEALIRSRAHDALEKVLPAYLRGCRWFRGTGQRLLAARIVESVPLRFGKRVAHLGLV
                     RVDYADHDPQTYVLPLAFAGEKQAQPIEDSDPAALIARLAQGHAERREPGVLYDALAD
                     PAFAQALLGVIAQRRRLAGTHGEVVGEARPILRRGGGRKPAVPNPRLVPGDLDNTTLI
                     FGDQWVLKMFRCVDEGPNPDAEIAAFLTDRDFAHTPPFGGLLEYRPRQGAPVTLGIVT
                     RFVPNTGDAWEAAQQALAEYLPRVAGEAAPALDAALTTRALLDLAAQPPPDAGARIGP
                     FLDAIRLLGRRTAELHLALGSDTERREFRPQPFTPYYQRALYQALRRLTSEALDALRR
                     QAAHLPEAVQPDA"
BASE COUNT          408 a          841 c          980 g          379 t
ORIGIN      
        1 cggcgtcggg ctgcacggcc tccggcaggt gggcggcctg gcgccgcagg gcgtcgagcg
       61 cctcgctggt gagccgccgc agcgcctgat acagggcgcg ctgataatag ggcgtgaagg
      121 gctggggccg gaactcccgg cgctcggtgt ccgacccgag ggccaggtgg agttcggcgg
      181 tgcgccggcc cagcaagcgg atcgcgtcca ggaaggggcc gatgcgcgcc ccggcgtcgg
      241 gcggcggctg cgcggccagg tcgagcaggg cgcgggtcgt gagcgcggcg tccagggcgg
      301 gggcggcctc gccggccacg cgcggcaggt actcggccag cgcctgctgg gccgcctccc
      361 aggcatcgcc cgtgttgggg acgaagcggg tcacgatgcc cagcgtgacg ggtgcgccct
      421 ggcggggccg gtattccagc agccccccga acggcggcgt gtgcgcgaag tcccggtcgg
      481 tgaggaaggc cgcgatctcg gcatccggat tcgggccctc atcgacacag cgaaacatct
      541 tgagcaccca ctgatcgcca aagatcagcg tcgtattgtc caagtcgccg ggcaccagtc
      601 gcgggttggg cacggccggc ttgcgtcccc cgccgcgacg caggatcggc cgcgcctcgc
      661 ccacgacctc gccgtgcgtg cccgcgaggc gccggcgctg ggcaatgacg ccgagcaacg
      721 cctgggcaaa cgccgggtcg gccagggcgt cgtagagcac gccgggttcc cgccgctcgg
      781 cgtggccctg agccaaccgg gcgatcagcg ccgcggggtc ggaatcctcg ataggctggg
      841 cctgcttctc accggcaaac gccagtggca agacgtaggt ttgcgggtcg tgatccgcgt
      901 agtccacgcg cacgaggccc aggtgggcca cgcgcttgcc gaaccgcaac ggcaccgatt
      961 cgacgatgcg cgccgccagc aggcgttggc ccgtgccgcg gaaccagcgg caaccgcgca
     1021 ggtaggccgg cagcaccttc tccagggcat cgtgcgcccg gctccggatg agggcttccc
     1081 agccgtcggg cgcggccagg gccggcaggg cggcctgctc agccggggcg gcgggcaccg
     1141 gggccggctc cagggcgaac cagtagaagc tgtgcggccc gagggtgagc agatacggca
     1201 actcgccgat gggcgggaag ggcacgcgtc cgaacaattc cacgggcacc gacccctgga
     1261 acgccgcgag atccagctcc acatgctgcg cgaagcgcga caggttcgcc acgaccagga
     1321 tctgctcgtc ttcgtaggcg cgcaggaaca ccagcacctt gcggttctcc ggatgaagaa
     1381 actccagcgt gccccgcccg aaggcacggt accgtttgcg catggcgatg agccggcgca
     1441 tccaggccag cagcgagtgg gggttgctct gctgcgactc gacctggacg ctctcgtggt
     1501 gatactcgtg gtcgacaatg agcggcaggt agagttgttg cgggttggcc tgggagaagc
     1561 cgccgttgcg gtcggcgctc cactgcatgg gcgtgcgcac gccgttgcgg tcgcccagat
     1621 agatattgtc gcccatgccg atctcatcgc cgtagtagat caccggcgtg ccgggcagcg
     1681 agaagagcag ggcgttcagc aactcgatgc ggcgccggtc gttgcgcagc aggggcgcga
     1741 gccggcggcg gatgcccagg ttcactcgcg cggcagggtc ctgggcgtag gcccggtaca
     1801 ggtagatgcg ctcctcgtcg gtgaccatct ccagggtcaa ctcatcgtgg ttgcgcagga
     1861 acagggccca ctgcgcggta tcggggatgg ccggggtctg ggccatgatg tcgatgatcg
     1921 gccagcgctc ctcgcggcgg atggacatga agaggcgggg catgagcgga aaatggaagg
     1981 ccatatggca ctcattaccc gtgccgaaat aggcgacggc gtcttcgggc cactggttgg
     2041 cctccgcgag cagcatccgg tcggggtagt gccggtcgat atgggcgcgc aactcttgca
     2101 ggaaggcatg ggtctcggac aggttctcgc cggtggtgcc ctcgcgctca tacaggtagg
     2161 gcaccgcgtc gaggcgcagc ccgtccacgc ccagcttgag ccagaaatcg accacctgca
     2221 gcatcgcctt gcgcaccggc gggtggtcga agttgaggtc gggctggtgg gcgtagaagc
     2281 ggtgccagta ataggcctcg gccaccggat cccagctcca gtttgaggcc tcgaagtcct
     2341 tgaagatcac acgcgccgcg ggatagcgtt cgggcgtgtc gctccagacg tagaaccggc
     2401 gccagttgct gccgggcggg gcctgccggg cgcgctggaa ccaggggtgc tcctcggacg
     2461 tgtggttgag caccagttcg gtgatcaccc gcaagccgcg gtcgtgcgcg gcgcgcagga
     2521 agcgcttgaa atcgcccaac gtgccgtaga gcgggtggac ggcggtgtaa tcggcgatgt
     2581 cgtagccgtc gtcgcgcagg ggggacac
//
LOCUS       DASUYC010000712         3738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_2040613, whole genome shotgun sequence.
ACCESSION   DASUYC010000712 DASUYC010000000
VERSION     DASUYC010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2040613"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VF276_11615"
     CDS             <1..338
                     /locus_tag="VF276_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="PucC family protein"
                     /protein_id="HEX6607550.1"
                     /translation="LPTPGLFPEAAFNTVFYIGIGFGIGYGTYQAVDWALGTDVLPNK
                     ETGAAKDMGVWHISFVLPQVLATLISGWLLYAFAAFEPTTRYGLVFAASILYFILGTV
                     LVRNVRGAR"
     gene            complement(532..>3738)
                     /locus_tag="VF276_11620"
     CDS             complement(532..>3738)
                     /locus_tag="VF276_11620"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEX6607551.1"
                     /translation="RNLRRVLCAVLLTGCSLISVAQAHESVPNPPALAAAQSLDLTVI
                     PVSVGEVIVQWAPPADSAATGYTLWRDDRVLVAVDAQTLSYRDTAVQPARLYRYRVAA
                     RVLGSGAPVESPTVAVRTPAWPELPDTLPPAPPDDFDAVSTAAGILLDWYPGADDTEV
                     TAYEIRRNGQLLTTVNSGILRYVDRQVTPATVYTYTITAVDAVGRRSDPSPAAPVRMA
                     GGPALATPPVDPPPAAAGPAGYSSHLQRYPYLTDVVDVYATINWATDNSGTTGTVTWG
                     EAGSESCDAHPAPATKTSIIVNGITEYQWKASLTLSADTEYCYRVYLGGTDLLGTDPS
                     PHFWTQLPTGSDTPYSFAVFGDWGSVDEMGANADQANVMAQIAASGARFALTTGDNAY
                     TSGSQSNYGDLTQTGPGISAVFGPAFWTVAGASIPLFPAIGNHGLSRSDTNHPHLLNW
                     PQDRAVSTSNGRYVRDLYCCVNESNPASYPSTWYAFDAGRARFYVLTAAWADGNVGNA
                     SPYANDYAAHWTPDSPEYQWLENDLATHNTALKFAFFHYPLYSDNSSETSDPFLQGAN
                     SLEGLLSRYGVDIAFNGHAHIYQRSFSSGPNGLIAYLTGGGGGKVEPVSHCSAIDAYS
                     IGWSYSANGGAGGGSACGGATRPTARAQVFHFLLVTVNGTQVTVAPTDSLGRTFDVQI
                     YDFGEGPNPPSPTPTAIATTTSIPTTTATATPIPSATATPTLTYTPAPTRTATPTDTP
                     TDTPTWTPTRTPTATPTATPTWTPTWTPTATPTDTPTRTPTATATATPTRTPTRTPSA
                     TPTRRPTRTPTHTPSTTPAPTRTATPGPTFTPVPDATATRTPLPVTVTPVMTPSRTPS
                     PPACTLSFVDVPASNPFYSYITWLACRGYVSGYSCGAPGESCPGAYFRPSADVTRGQL
                     LKMVVNAAGWPAADGGQTFEDVPPGSAFYPFIETGARQGIISGYTCGGPGEPCVAPGN
                     RPYFRPTRSITRGQLSKVLALARDYPLPDPTTPAFADVPPGHPFYPYIEALAERGIIS
                     GYTCGGPGEPCPARYFRPDAGATRGQVSKVVAGAYGMP"
BASE COUNT          547 a         1145 c         1408 g          638 t
ORIGIN      
        1 cgctgcccac gcccggcctc ttcccggagg ccgccttcaa cacggtcttc tacatcggta
       61 tcggcttcgg gatcggctac gggacgtacc aggcggtgga ctgggcgctg ggcaccgatg
      121 tgctgcccaa caaagagaca ggcgcggcca aggacatggg cgtatggcac atctcgttcg
      181 tactgccgca ggtgctcgcc acgctgatca gcggctggct gctgtacgcc ttcgccgcct
      241 tcgagccgac gacccggtat ggcctggtgt tcgccgcgtc gatcctctac ttcatcctcg
      301 gcacagtgct ggtgcgcaac gtgcgcggag cacgctgagc ctcgtccgca gcagttcggg
      361 cacacgtaaa cagggaccgg gatgaggcaa tcccggtccc tgtcgctttt ccgccgcgcc
      421 ctgcgttagt gcagattcaa gtgacccgtt gtgtgtagcg gcgcgatgaa tcgcgcccgt
      481 actcgttggt ctttagcgta ttctgtcccc aacaagttgc ggaaccctgc gctaaggcat
      541 gccgtaagcg ccggcaacca ccttgctgac ctgcccccgc gtggcgccgg catcgggccg
      601 gaagtagcgg gccgggcagg gttcgccggg acccccgcaa gtatagccgc tgatgatccc
      661 ccgctctgcc agcgcctcga tatagggata gaacggatgc ccgggcggca cgtcggcgaa
      721 agccggcgtg gtggggtcgg gcagcgggta gtcccgcgcc agggcgagca ctttgctcag
      781 ctggccgcgg gtgatgctgc gggtggggcg gaaatagggc cggttgcccg gcgccacgca
      841 cggctcgccc ggcccaccgc acgtgtaccc gctgatgatg ccctggcgcg ccccagtctc
      901 gatgaacggg tagaaggcac tgcccggcgg cacatcctca aaggtctgcc cgccgtccgc
      961 cgcgggccag cccgccgcat taaccaccat cttcagcaac tgcccgcgcg tcacatccgc
     1021 gctgggccgg aaataggcac cggggcacga ctcgcccggc gcgccgcagg agtagccact
     1081 cacatacccc cggcaggcca gccaggtgat atagctatag aaggggttgc tcgccggcac
     1141 gtcgacaaaa ctgagcgtgc aggcgggcgg ggacggcgtg cgcgagggcg tcatcaccgg
     1201 cgtaaccgtc accggcagtg gagtgcgcgt cgccgtggcg tccgggaccg gcgtgaaggt
     1261 gggccctggt gtcgccgtac gggtcggcgc cggggtggtg ctgggcgtat gggtcggagt
     1321 gcgggtgggc cgcctggtcg gcgtcgcact cggagtccgc gtaggcgtgc gggtcggtgt
     1381 ggcggtcgct gtggccgtcg gggtacgagt gggtgtgtcg gtcggcgtcg cggtgggcgt
     1441 ccaggtcggc gtccaggtcg gtgtagcggt cggtgtggcg gtgggagtgc gggtgggcgt
     1501 ccacgttggt gtgtcggtcg gtgtgtcagt cggcgtcgca gtgcgtgtcg gtgccggcgt
     1561 gtaggtcagc gtgggcgtcg cggtcgcgct aggaatagga gtcgccgtcg cggtcgtggt
     1621 ggggatgctc gtggtggtcg cgatggccgt gggcgtgggc gacggcggat tggggccctc
     1681 gccgaagtcg tagatttgca cgtcgaacgt gcggcccagc gagtcggtgg gggccaccgt
     1741 gacctgggtg ccgttcaccg tgaccagcag gaagtggaac acctgcgccc gcgcggtggg
     1801 ccgggtggcg ccgccgcagg cgctgccgcc gccggccccg ccgttggccg aatagctcca
     1861 gccgatgctg taggcgtcga tggcgctaca gtggctgacc ggttccacct tgcccccacc
     1921 gcccccggtc aggtaggcga tcagcccgtt cggtcccgac gagaagctgc gctggtagat
     1981 gtgggcgtga ccgttgaagg cgatatcgac gccgtagcgg ctgagcaacc cttcgaggct
     2041 gttcgcgcct tgcaggaagg ggtcggaggt ctcgctgctg ttgtccgagt acagcgggta
     2101 atggaagaag gcgaatttca gcgccgtgtt gtgcgtcgcc aggtcgttct ccagccactg
     2161 atattcgggg ctgtcggggg tccagtgggc ggcgtaatcg ttcgcgtagg ggctggcgtt
     2221 gccgacattg ccgtcggccc aggcggcggt gagcacgtag aagcgcgcgc gcccggcgtc
     2281 gaaggcgtac caggtgctgg gatagctggc cggattcgat tcgttgacgc aacagtagag
     2341 gtcgcggacg tagcgcccat tggaggtgct gacggcgcgg tcctgcggcc agttgagcag
     2401 gtgcgggtgg ttggtgtcgg agcgcgacag gccatggttg ccgatggccg ggaacagcgg
     2461 gatactcgcc ccggccacag tccagaaggc gggaccgaag acggcgctga tgccggggcc
     2521 ggtctgggtc agatcaccat agttgctctg gctgccggag gtgtaggcgt tatcaccggt
     2581 cgtgagggcg aagcgcgcgc cgctggcggc gatctgagcc atgacgttgg cctggtcggc
     2641 attagcgccc atctcgtcca cgctgcccca atcgccgaat acagcgaacg aatagggggt
     2701 gtcgctgccc gtgggaagct gggtccagaa atggggcgag ggatcggtgc ccaatagatc
     2761 ggtgccgccg aggtagacgc ggtagcaata ttcggtgtcg gcactcaacg tcagactggc
     2821 cttccattgg tactcggtga tgccgttgac gatgatgctg gttttggtcg ccggggcggg
     2881 gtgcgcgtcg caggactcgc tgccggcctc gccccaggtc acagtgccgg tggtgcccga
     2941 attgtccgtg gcccagttga tcgtggcgta cacgtcgacc acgtcggtta gatagggata
     3001 gcgctgcaag tgcgagctgt atcccgccgg gccggctgct gccgggggtg ggtccaccgg
     3061 gggcgttgcc agggccggcc caccggccat acgcaccggg gccgccgggg atgggtcgga
     3121 gcgccggccc acagcgtcca cggcagtaat ggtgtaggtg tagaccgtag ccggggtgac
     3181 ctggcggtcc acatagcgca ggatgccgct gttgacggtg gtgagcagtt gcccgttgcg
     3241 ccggatctcg taggccgtta cttcggtatc gtcggcgccc ggataccagt cgagcaggat
     3301 gcccgcggcg gtgctgacgg cgtcgaagtc gtcgggaggc gcgggcggca gggtgtccgg
     3361 cagttccggc caggcgggcg tccgcacggc caccgtgggc gactccaccg gcgccccact
     3421 gcccaggacg cgcgccgcca cccggtatcg atatagcctc gcgggctgca cggcggtatc
     3481 gcggtagctc aaggtttgcg catccaccgc gaccagcacc cggtcgtcac gccacagcgt
     3541 gtaacccgta gctgcggaat ccgcaggcgg cgcccattgg acaatgacct cgcccacact
     3601 gaccgggatc acggtcaggt cgagggattg tgccgccgca agcgcgggcg gattggggac
     3661 ggattcgtgg gcctgcgcga cgctgatcag gctgcacccg gtgaggagga cggcgcacaa
     3721 gacgcgccgg agattgcg
//
LOCUS       DASUYC010000713         2241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1956756, whole genome shotgun sequence.
ACCESSION   DASUYC010000713 DASUYC010000000
VERSION     DASUYC010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1956756"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VF276_11625"
     CDS             complement(<1..413)
                     /locus_tag="VF276_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607552.1"
                     /translation="MPGQTWLLLRLQGRGALNTFRRTFGPADKAGGVIFVAVLALAVT
                     LAGSIAASFAAGAAIRFPQAGVPRLFPAAVLGLGALLLVLLALRGAAGALFFDRDLSL
                     LLSAPVDPRAVFLSKLLAGLARGAILPVLGMAAPLV"
     gene            complement(419..1645)
                     /gene="lhgO"
                     /locus_tag="VF276_11630"
     CDS             complement(419..1645)
                     /gene="lhgO"
                     /locus_tag="VF276_11630"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HEX6607553.1"
                     /translation="MSKRPWDVIIIGAGIVGLATARELLTRYPHLRLAVLEKDRQIAD
                     QQTGHNSGVMHAGIYYKPGSLKATLCTTGVRMLFDYCEAHGIAVDRCGKVIVATNEEE
                     LPRLDDLYARGQANGVPGLERIGPERLREIEPHAAGVAAVYSPNTAIVDFRQVARAYA
                     ADVRKAGGAVVTDCEVTAIRVLPEGKIRLRTTRGGLTTRSLISCGGLQSDQLAIMAGA
                     PRDVQIVPFRGDYYVLKPEKRYLCRGLIYPVPDPALPFLGVHFTKRIDGDVWAGPNAV
                     LAFAREGYRRVDVSIPELAGLVAYPGFWRMAREYWRVGVDEMYRDFVKSAFVRALQRY
                     VPELDSRDVAPGPAGVRAQAVTADGVLADDFLVYAADNMVHVRNAPSPAATSSLAIAR
                     LIADRAVETCAVLQPA"
     gene            complement(1683..>2241)
                     /locus_tag="VF276_11635"
     CDS             complement(1683..>2241)
                     /locus_tag="VF276_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607554.1"
                     /translation="TTVGVASQTAREVVGKLDQDRPVEVVGKSNQGAQSGVVGKNNRG
                     GRIKQPGWLDNPPEVVGESDSQQTDQETESNDDSNRSAPEFRKDTTSAPPYSPYIAGI
                     VIDHSRELGDLAHGPANVTQALRLWQQSALAEDYFVACLHEARRRVHTYQGKQGLGGI
                     QNRMAYYFRVVADLARGEGTAAAAP"
BASE COUNT          362 a          762 c          761 g          356 t
ORIGIN      
        1 accagcgggg cggccatgcc cagcaccggc aggattgcac cgcgcgccaa ccccgccagc
       61 agcttgctga ggaatacggc ccgcgggtcc accggcgcgc tcaagagcaa cgagaggtcg
      121 cggtcgaaaa agagggcgcc ggccgcgccg cgcagggcca gcagcacgag cagcagcgcg
      181 cccaggccca gcaccgccgc cgggaagagg cgaggcacgc cggcctgggg gaagcggatc
      241 gccgcgcccg ccgcgaacga ggcggcgatg ctacccgcca gggtcacggc cagcgccagg
      301 acggcgacaa agatcacccc gcccgcctta tcggcggggc cgaaggtgcg ccggaaggtg
      361 ttcagcgcgc cgcggccttg caggcgcagc agcagccacg tctggccggg catcggcgtc
      421 aggcgggttg caagaccgcg caggtctcga cggcgcggtc ggcgatgagg cgggcaatcg
      481 ccagcgacga cgtggcggcg ggcgacggtg cgttgcgcac atggaccatg ttgtcggcgg
      541 cgtagaccag gaagtcgtcg gccagcacgc cgtcggcggt gacggcctgg gcgcgcacgc
      601 ccgccgggcc gggcgccacg tcgcggctat cgagttcggg cacatagcgc tgcagcgcgc
      661 gcacgaaggc cgatttcacg aagtcgcggt acatctcgtc cacgccgacg cgccagtact
      721 cgcgggccat gcgccagaag ccgggatagg ccaccaaccc ggcgagttcg gggatactca
      781 cgtccacccg gcgatagccc tcgcgggcga aggccagcac ggcgttgggg ccggcccaga
      841 catcgccgtc gatgcgcttg gtgaagtgca cgcccaggaa gggcagcgcc gggtcgggca
      901 ccgggtagat aaggccgcgg cagaggtagc gcttttcggg cttcagcaca tagtagtcgc
      961 cgcggaaagg cacgatctgc acgtcgcgcg gggcaccggc catgatcgcc agttggtccg
     1021 attgcagccc gccgcagctt atcaggctgc gcgtagtcag gcccccgcgc gtggtgcgca
     1081 ggcggatctt gccctccggc aagacgcgga tggccgtgac ctcgcaatcc gtgacgacgg
     1141 cgccgccggc tttacgcaca tcggcggcgt aggcgcgcgc cacctggcga aagtcgacga
     1201 tggccgtgtt gggcgagtag acggcggcga ccccggcggc gtggggttcg atctcgcgca
     1261 agcgctcggg gccgatgcgt tccaggccgg gcacgccgtt ggcctggccg cgcgcataga
     1321 gatcgtccag gcgcggcagc tcttcctcgt tcgtggcgac gatgaccttg ccgcaacggt
     1381 ccaccgcgat gccgtgggct tcgcagtagt cgaacagcat gcgcacgccc gtcgtgcaga
     1441 gcgtcgcctt cagcgagccg ggcttgtagt aaatgccggc gtgcatcact ccgctgttgt
     1501 gccccgtctg ctgatcggcg atctgccggt ccttctccag cacggccagg cgcaggtgcg
     1561 gatagcgggt caacagttcg cgcgccgtcg ccagcccgac aatgccggcg ccgatgataa
     1621 tcacgtccca cggtcgtttg ctcatgcttg cttccttgtg ctgcgggacc ccggcggcgc
     1681 gctcacggtg cggcggccgc ggtcccctca ccgcgcgcca gatctgcgac gacgcggaaa
     1741 taataggcca tgcggttctg aatcccgccc agcccctgct taccctggta cgtatgcacc
     1801 cgccgtcgcg cttcgtgcag gcaggcaaca aagtagtcct cggccaacgc gctttgctgc
     1861 cagagccgta acgcctgggt gacattagcc gggccatgag ccaggtcgcc cagttcgcgg
     1921 gaatggtcga tgacgatgcc cgcgatatac ggagaatacg gcggcgcgct cgtcgtgtct
     1981 ttcctgaatt cgggtgcaga ccgattcgaa tcgtcgttgg actctgtttc ttggtctgtt
     2041 tgttgtgagt cggattcgcc aaccacctcc ggcggattat ccaaccaccc tggttgtttt
     2101 atccgaccac cccggttgtt ttttccaact accccgctct gtgccccctg gttcgatttt
     2161 ccaactacct ctaccggacg atcctggtct aattttccaa ctacctcccg cgccgtctgt
     2221 gacgcaactc caaccgtggt g
//
LOCUS       DASUYC010000714         5939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1263300, whole genome shotgun sequence.
ACCESSION   DASUYC010000714 DASUYC010000000
VERSION     DASUYC010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5939
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1263300"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VF276_11640"
     CDS             <1..345
                     /locus_tag="VF276_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6607555.1"
                     /translation="PHPSLIIADEPVSMIDASRRMIIINLFLALKQEYNTSFLYITHD
                     LATAYYISDYIAVMNHGTVVEFGEAKQVLSNPQHEYTQLLLDSIPRLDHKWMDEPYLD
                     PNTFARPAESRA"
     gene            403..1920
                     /locus_tag="VF276_11645"
     CDS             403..1920
                     /locus_tag="VF276_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HEX6607556.1"
                     /translation="MSAAREVEQWGIFELALQGPGEGNPFLEVELTTRFTYKHRAVEV
                     DGFYDGDGIYRVRFMPDTPGTWSYRTESNSAALHGAEGTFTCVAPTAGNHGPVRVANT
                     YHFAYADGTPYKQIGTTCYVWTHQGDALEEQTLATLREAPFNKLRMCVFPKSYAFNNN
                     EPPYYPFPVLSKGPARWQGGYMVEGSEGWSFDWSRFEPEFFRHFEQRVGDLRDLGIEA
                     DIILFHPYDRWGFATMDAATDDRYLRYVVARLAAYRNVWWSMANEFDFIKNKTLTDWD
                     RFFRIVQESDPYGHLRSIHNGWVLYDHSQPWVTHQSIQKWDLENVALWREQHKKPVVV
                     DECAYEGNIPQRWGNITGEEMVRRFWEGTAQGGYVGHSDTFLDPDEIVWWSKGGTLHG
                     QSAPRLAFLRQILEEGPAAGLDPFDRRASVASFFPCAGHAHEYYLVYFGNHQPAQMPL
                     VLPEGEQYRGEILDTWAMTRTPLAEPVQNGTVISLDQKPYQALVLRRSGAGHAEG"
     gene            1907..3214
                     /locus_tag="VF276_11650"
     CDS             1907..3214
                     /locus_tag="VF276_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 140 protein"
                     /protein_id="HEX6607557.1"
                     /translation="MLKVSDNRRFLVQDDGTPFFYLGDTAWELFHRLSYSEAERYLRD
                     RAAKRFTVIQAVALAEIDGLHTPNANGDTPLHDDDPTRPNEAYFAHVDKVVDLAASLG
                     LYIGMLPTWGDKWNQKWGVGPEIFTPQNARAYGLWLARRYAAQPIIWIAGGDRPIESE
                     NHRLILRALAEGLREGDGGRHLIAMHTWGPHSTAEYVHDEPWLDFHTCQSGHARNSAN
                     YRFIEADYARRPLRPCMDAEPGYEDMPDNIRNLDGGYLDDWDVRKAIYWALFAGAHGH
                     TYGCNPVWQMWQPGRKQLIWPRRPWYEAIHLPGAGQVQYARALIESRPFLSRIPDQSL
                     IASDAGEGTHHVQATRDSAGTYALVYLPAGDDVELDLSKLTGDQLRAYWFDPRTGVAQ
                     HIGVLQRQDRMRFTPPDGGLDWVLALDDPAAGYPAPGSRPLLG"
     gene            3254..4252
                     /locus_tag="VF276_11655"
     CDS             3254..4252
                     /locus_tag="VF276_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011093517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX6607558.1"
                     /translation="MQNYEFGAGLWMFQQFVDRYATDAYGPPVSTLEAIERAAAVGDI
                     KVLDINYPFAGDGVTVAQVRDTLARTGLRAYAITPHLYMREFQQGSFTNPDPAVRQRA
                     IDLGQQAVEIAHQLDAKYVKFWPGQDGFDYPFQVDYRRLWDDSVAGVRAVAESAPDMQ
                     FAIEYKAKEPRVQMFFGSAARTLLAIEDMGVDNVGILLDLGHSLFAKETPAEVVHLVA
                     RRGKLVSVEVNDNWREWDDDMAVGAVHLIETLEFLHALRQVHWQGPILLDQFPFREDP
                     IAAAHSSIRTIRALDGVLDRIDLNALAAAQDRQDALEAQRIVMEMLLGAPVTEATR"
     gene            4249..5097
                     /locus_tag="VF276_11660"
     CDS             4249..5097
                     /locus_tag="VF276_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020050234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX6607559.1"
                     /translation="MIDTLQVDTQQVEKLRKLASSVRRRDLQTVHKAKLGHVGGEMSA
                     IDILVTLYFAVLRVDPARPADPDRDRFILSKGHSAVALYTTLAHRGFIPMEQLATFAQ
                     PLSLLNGHPDRNKVPGVETNTGPLGHGLPVAVGAALAAKLQDCSWRVFVLTGDGELQE
                     GSNWEAAMAAAHYKLDNLTVIVDRNRLQQGDWTEQTMHLEPLADKWRAFGWAVSEVDG
                     HNIAQLLQTLERLPFEPGKPSCIIAHTHKGQGVSLMRDKAAWHHKVPTAEELTLALHE
                     LDEVTS"
     gene            5094..>5939
                     /locus_tag="VF276_11665"
     CDS             5094..>5939
                     /locus_tag="VF276_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018097432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX6607560.1"
                     /translation="MTQLYDCRDAFENKLRELASQNQRIVAVVNDSVGSSKLNAFGQM
                     YPVRLVNVGIAEQNMVGVGAGLANGGLIPFVCGASPFLTGRALEQIKTDLAYSNQNVK
                     LCGMSSGMAYGELGPTHHSIEDMAWLRAIANMTVIVPADPIETAQAVAVAAQTPGPMF
                     IRISRLPVPNVHGPDYQFTIGKAALLREGRDITLITNGVLVPRALEAAEMLAREGVQA
                     RVLNMSTVRPLDQAAVLAAAAETGGIVTAEEHTVFGGLGSAVAEVVVQHRPVPMRILG
                     VPGVFA"
BASE COUNT         1114 a         1955 c         1857 g         1013 t
ORIGIN      
        1 ccgcatccca gtcttatcat cgccgacgaa ccggtgagca tgatcgatgc gtcccggcgc
       61 atgatcatta tcaacctgtt cctggcgctc aagcaagagt acaacacgag ctttctctac
      121 atcacccatg atctcgccac cgcctattac atcagcgact acatcgccgt gatgaaccac
      181 ggcaccgtgg tcgaattcgg ggaagcgaag caggtgctgt ccaatccgca gcatgaatat
      241 acccaactgc tgctcgactc catcccccgg ctggatcaca agtggatgga tgagccctac
      301 ctggacccga acacgtttgc ccgccccgcc gaaagtcggg cataaggagc agcagcaatg
      361 acagtgacca acaacatacg tgaaacccaa ccaactgcag ctatgagcgc ggcgagggaa
      421 gtcgagcagt ggggcatatt cgagctagcc ttgcagggac ccggcgaggg caacccgttt
      481 ctggaagtgg agcttacgac gcggttcacc tacaagcacc gtgccgtcga agttgatggc
      541 ttctatgacg gcgacgggat ctaccgcgtc cgcttcatgc cggacacgcc gggcacctgg
      601 agctatcgca cggagagcaa cagcgcagcg ctgcatggcg ccgaggggac gtttacctgc
      661 gtggccccca cggcgggcaa tcacggcccc gtgcgggtgg cgaacaccta ccacttcgcc
      721 tacgccgacg gcacgcccta taagcagatc ggcactacct gctacgtctg gacgcaccag
      781 ggtgacgcgc tggaggagca gacgctggcg acattgcgcg aggcgccgtt caacaagttg
      841 cgcatgtgcg tgttccccaa gagctacgcc ttcaacaaca acgagccgcc ttattacccc
      901 ttcccggtct tgtcaaaagg gcccgcccgc tggcaggggg ggtatatggt ggagggatcg
      961 gagggctggt cgttcgattg gagccgcttc gagccggagt tcttccgcca ctttgagcag
     1021 cgcgtgggtg acctgcggga cctgggcatc gaagccgata ttatcctgtt ccatccctac
     1081 gaccgctggg gattcgccac catggacgcg gcgaccgacg accgctacct gcgctacgtg
     1141 gtggcccggc tcgccgccta ccgcaacgtc tggtggtcga tggcgaacga gtttgatttc
     1201 atcaagaaca agaccctgac tgattgggac cgcttcttcc gcattgtgca ggagagcgac
     1261 ccctatgggc acctgcgctc gatccacaac ggctgggtgc tttacgacca ctcccaaccc
     1321 tgggttacgc accagagcat ccagaagtgg gatctggaaa atgtcgcgct ctggcgtgag
     1381 cagcacaaga agccggtcgt ggtcgacgag tgcgcgtatg agggcaatat cccccagcgc
     1441 tggggcaata tcaccggcga ggagatggtg cggcgcttct gggagggcac cgcgcagggc
     1501 ggctacgtcg ggcacagcga cacgttcctc gaccctgacg agatcgtctg gtggtcgaag
     1561 ggcgggacct tacacggcca gagtgcgcca cgcctggcct tcctgcgtca gatccttgaa
     1621 gaaggaccgg cagccggtct cgatccattc gaccggcggg cgagtgtggc atccttcttt
     1681 ccgtgcgcgg ggcacgcgca tgaatactac ctcgtctatt tcggcaatca ccaacccgcg
     1741 caaatgcccc tggttttgcc ggaaggtgag cagtaccgcg gcgagatcct cgatacgtgg
     1801 gcgatgacgc gcaccccgct ggccgagccg gtgcaaaacg gcacggtgat cagcctggac
     1861 cagaagccct accaggccct cgtcctgcgc cgtagcggag ccggccatgc tgaaggttag
     1921 cgacaaccgc cgcttcctgg tccaggatga cggcaccccg ttcttctatc tcggcgacac
     1981 cgcctgggaa ttgttccacc gcctgagcta cagcgaagcc gagcgctacc tgcgggaccg
     2041 cgccgccaag cgtttcaccg tgatccaggc ggtggcactg gccgagatcg acgggctgca
     2101 tacgccgaac gcgaacggcg atacgccgct ccacgacgac gacccgaccc ggcccaatga
     2161 agcctacttc gcccacgtcg ataaggtggt cgatctggcc gcatcgctgg ggctctacat
     2221 cgggatgttg ccgacctggg gcgacaagtg gaaccagaaa tggggggtcg ggccggagat
     2281 ctttacgccg cagaacgccc gcgcctacgg tctttggctg gctcgccgct acgccgccca
     2341 accgatcatc tggatcgccg gcggcgaccg gccgattgag agcgaaaacc accgcctgat
     2401 cctgcgggcg ctggccgagg ggttgcggga gggcgacggc ggccgccacc tgatcgccat
     2461 gcacacctgg gggccgcact ccaccgccga gtacgtccat gacgagccct ggcttgactt
     2521 ccacacctgc cagagcggcc acgcccgcaa ctcggctaac tatcgcttta tcgaagccga
     2581 ctatgcgcgc cggccgctgc gaccctgtat ggatgccgaa ccgggctatg aggatatgcc
     2641 ggacaacata cgcaatctcg acggcggcta cctggacgat tgggatgtgc gcaaggcgat
     2701 ctactgggcg ctcttcgcgg gggcgcacgg ccacacctat ggttgcaacc cggtctggca
     2761 gatgtggcaa cccggacgta agcaattgat ctggccgcgc cgtccctggt acgaggccat
     2821 ccacctgccc ggagcgggcc aggttcagta cgcgcgtgcg ctgatcgaat cccggccctt
     2881 cctcagccgc atccccgacc aatcgttgat cgcgtcggac gccggcgagg gcacccatca
     2941 tgtgcaggca acccgcgaca gcgccggcac ctatgcgctg gtctacctgc ccgccggtga
     3001 cgacgtggaa ctggacctca gcaaactgac aggcgaccaa ctgcgcgctt attggttcga
     3061 tccacgcacc ggggtggcgc agcatatcgg cgtcctacaa cggcaggacc gtatgcgctt
     3121 tactccgccg gatggcggcc tggactgggt gttagctctg gatgatccgg cagcgggcta
     3181 tccagcgccc ggcagcaggc ccttattggg atagtcgcca acgactgtca agcattcagt
     3241 aaggaggttc taaatgcaga attacgaatt cggggcgggg ctatggatgt tccagcagtt
     3301 tgtcgaccgc tacgccaccg acgcctacgg tcccccggtc agcacgctcg aagctattga
     3361 gcgcgccgcc gccgtcggcg atatcaaggt gctcgacatc aactacccgt tcgcgggcga
     3421 cggcgtcacc gtcgcccagg tacgcgatac gctcgcacgc acgggactgc gcgcgtacgc
     3481 cattaccccc catctctaca tgcgcgagtt ccagcagggc agctttacca accctgaccc
     3541 cgccgtgcgc cagagggcca tcgacctggg ccagcaggcc gtcgagattg cgcatcaact
     3601 ggatgccaag tacgtgaagt tctggcctgg ccaggacggg tttgactacc ccttccaggt
     3661 cgattatcgt cgtctctggg acgattccgt cgcgggcgtg cgcgccgtcg ccgaaagcgc
     3721 gcccgatatg cagttcgcca tcgaatacaa ggccaaggag ccgcgtgtcc agatgttctt
     3781 cggctccgcc gcccgcacgc tgctcgccat cgaggacatg ggggtggaca atgtgggcat
     3841 cctgctggac ctgggccatt cgctgttcgc caaagagacc cccgccgagg tggtgcacct
     3901 ggtggcccgc cggggcaagc tggtctcggt tgaggtgaac gacaactggc gcgagtggga
     3961 cgacgatatg gccgtgggcg cggtgcatct gatcgagacg ctggagttcc tgcacgcgct
     4021 gcgccaggtg cactggcagg gcccgatcct cttggaccag ttccccttcc gcgaggaccc
     4081 gatcgcggcg gcgcacagca gcatccgcac cattcgcgcc ctcgacggcg tactcgaccg
     4141 cattgacttg aatgccctcg ccgccgccca ggaccgccag gatgccctcg aagcccagcg
     4201 catcgtcatg gagatgctgc tgggcgcccc ggtcacggag gccacaagat gatcgataca
     4261 ctgcaggtag atacccagca ggtggaaaag ctacgcaagc tggccagttc ggtgcgccgc
     4321 cgcgacctgc aaaccgtaca caaggccaag ctggggcatg tgggcggcga gatgtcggcc
     4381 atcgacatcc tggtgacgct ctatttcgcc gtgcttcgcg tcgatcccgc ccgccccgcc
     4441 gatcctgatc gcgaccgctt catcctcagc aaaggccatt cggcggtggc gctctatact
     4501 acgctggccc atcgcggctt catccccatg gagcaactcg ccaccttcgc ccagcccctc
     4561 tccctgctca atgggcaccc cgaccgcaat aaggttcccg gtgtcgagac caacaccggc
     4621 ccgcttgggc atggcctccc cgtcgcggtg ggggcggcgc tggccgccaa gctgcaggac
     4681 tgtagctggc gggtgttcgt gcttaccggc gacggggagt tgcaggaggg cagcaactgg
     4741 gaggccgcca tggccgccgc ccactacaag ctcgacaacc tgaccgtgat cgtcgatcgc
     4801 aaccgcctgc aacagggcga ctggaccgag cagaccatgc acctggaacc gctggccgac
     4861 aaatggcgcg ccttcggctg ggccgtctcc gaggtggacg gccacaatat cgcccaactc
     4921 ctgcagaccc tggagcgcct gccgtttgag ccgggcaaac ctagttgcat catcgcccac
     4981 acccacaaag ggcagggcgt gtcgctgatg cgcgacaagg ccgcctggca tcataaggtg
     5041 cccactgccg aggagctcac gctcgctctc cacgaattgg acgaggtgac ctcatgaccc
     5101 aactctatga ttgccgtgac gcttttgaga acaagttgcg cgaactggcc tctcagaacc
     5161 agcgcatcgt cgccgtggtc aacgactcgg ttggctccag caagctgaac gccttcggcc
     5221 agatgtaccc ggtgcgactg gtgaacgtgg gcatcgccga gcagaacatg gtgggcgtag
     5281 gggccgggct cgccaatggc gggctcatcc cttttgtttg cggcgcgtcg ccgttcctga
     5341 ccggacgcgc tttggagcag atcaagaccg acctggccta ctcgaaccag aacgtcaagc
     5401 tctgcggcat gagcagcggc atggcctacg gcgaactggg gccgactcac cattccattg
     5461 aagatatggc ctggctgcgg gctatcgcca acatgacggt gatcgtcccg gcggatccca
     5521 tcgagacggc gcaggcggtc gcggtggcgg cgcagacgcc cggcccgatg tttatccgca
     5581 tcagccgctt gcccgtgccc aacgtgcatg gccctgacta ccagttcaca atcggcaagg
     5641 cggcactgct gcgtgagggt cgcgatatca cgctcatcac taacggcgta ctagtaccaa
     5701 gggccttgga agcagccgag atgctggcgc gggaaggggt gcaggcacgg gtgctcaaca
     5761 tgagtaccgt gcggccgctg gaccaggccg cggtgctggc cgcggcggcg gaaaccggcg
     5821 gcattgtgac cgcggaggag cacaccgtct tcggtgggct gggcagcgcg gtggccgagg
     5881 tcgtcgtcca gcaccggccc gtgcccatgc gcatcctggg cgtgcccggc gtcttcgcc
//
LOCUS       DASUYC010000715         3832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_840512,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000715 DASUYC010000000
VERSION     DASUYC010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840512"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VF276_11670"
     CDS             <1..315
                     /locus_tag="VF276_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607561.1"
                     /translation="IIALTLMLYGMGIARAVGFPRWSRWFAATTVLIGFAQLIDGVSV
                     GYNGFAMPTTPPVLWPVVSILIGPVFLLWGLALGVFLWQQAGQAAPAPGAVFQVAAEP
                     GA"
     gene            419..1516
                     /locus_tag="VF276_11675"
     CDS             419..1516
                     /locus_tag="VF276_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607562.1"
                     /translation="MAAALNSQQIMVREEVAPDPYASSWLDRLTASVDRLPGPAWLVY
                     LLTGVLVLLLFTVLKWVDGTFPVGHVELYHLVFVGTPLYALAVLHLLDQVAGQALEDF
                     RPVLITDAATVARLRYQLTTLPATPALVVTLGCVVSAFLVWWNVGDVQEALAARPWAF
                     LPITALEAAITLSTWVLAGLFIYHTVHQLRTVTAIYAEHTRINLFTLGPLYTFSRLTA
                     LTAIAPMPLFLVDYLGWPGQGNEPITTGTLGLWGLFGLLSVCTFVLPLLGVRRLLAQE
                     RAQAQLDLAARLRTLLAGLHQQVSSGAASADSTTATKNLLDSLLAEQGLLNKVSSWPW
                     APETPRALATALLLPVLVVIISELVKRLFGL"
     gene            1650..2672
                     /locus_tag="VF276_11680"
     CDS             1650..2672
                     /locus_tag="VF276_11680"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6607563.1"
                     /translation="MATVTNTLPVPTTATRMGRRVLRGIARGGGVVLGLIAGLALAGA
                     AYEAIAARGDAHTYPPPGQMVDVGGYRLHIHCVGTGSPTVVLDAGLSRTSLDWSLVQP
                     AIGQTTRVCAYDRAGMGWSDPGPAPRTPAQIAGELHTLLGNAGLAGPYVLVGHSLAGK
                     NVRLFALAHPAEVASLVLVDARSEYVDAHTSPAEAAAFQQALAQQGTIYGLARAVGLV
                     RLVGASQEGAPELPAETRQAMALFSTGQRGLATTAAEGQQRAADDGQLQAAPGLGARP
                     LIVLAAGQNMEQTPYWADAQRQQAALSTNGRLIVAAGSGHGINLERPALVIDAVRQVV
                     KQVRGH"
     gene            2779..3495
                     /locus_tag="VF276_11685"
     CDS             2779..3495
                     /locus_tag="VF276_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6607564.1"
                     /translation="MALATWWRGDALPTLDPLPDFHVEASRDERLIARVTGLDPEQVR
                     ERLAGGHQPYLASIATAPASYGWLAQQEAEIGELGLRFALPPVDRYLWDFATLPAWQG
                     YGIYPRLLQSILRQQALPVERMWIIYAPENTPSGAGMHKAGLLPVGHLSFRSDGTVEL
                     AASGAPQRAQVGADLLGVPISESVLAPCWACGRVTSPPATHVDATGCWPPRPSTATRC
                     TCAVELQPAKTIATAGAENQ"
     gene            complement(3623..>3832)
                     /locus_tag="VF276_11690"
     CDS             complement(3623..>3832)
                     /locus_tag="VF276_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607565.1"
                     /translation="TLHRRADTGDLTEFAALKAVLDGLVTEQGMIDKLRTWPWRTETV
                     AGFGVAFFVPMIFWFVQRVLERLGV"
BASE COUNT          575 a         1279 c         1317 g          661 t
ORIGIN      
        1 attatcgccc tgacgctgat gctctacggg atggggatcg cgcgtgcggt tggcttcccg
       61 cgctggtcgc gctggttcgc ggcgacaacc gtgctcatcg gttttgcgca actgatcgat
      121 ggggtgagtg tgggttacaa cgggttcgcc atgccaacga ccccgccggt cctatggccc
      181 gtcgtgagta tcctgattgg accagtgttc ctgctctggg ggttggcatt gggcgttttc
      241 ctgtggcagc aggccggcca agcggcccca gcgccgggcg ccgtgttcca ggtggcggca
      301 gaaccggggg cgtgaccgga ataggagggg gcccgcgcgg tccaacccgg cccccaggtt
      361 actgccggct gcaacggatc tgcggccggc agcaagccgc aggacaggag gtgcagccat
      421 ggccgcggcc ctcaactcgc aacagatcat ggtacgtgaa gaggtggcgc cggatcccta
      481 cgcctctagc tggctcgacc ggctcactgc tagtgtggat cgactccccg gtcccgcctg
      541 gttggtgtac ctgctgaccg gtgtcctggt gctgctgctg ttcaccgtgc tcaagtgggt
      601 cgacggcacg ttcccggtcg ggcacgtcga gttatatcat ctggtgtttg tcggtacgcc
      661 gctctacgcc ctggccgtgc tgcacctgct ggatcaggtg gccgggcagg cgctggagga
      721 ctttcgcccc gtgctgatca ccgacgccgc cactgtagcg cgcttgcgct atcaactcac
      781 cacgctgccg gcgacgcccg cgctggtggt tacgctcggc tgcgtggtga gtgcgttcct
      841 ggtttggtgg aatgtggggg acgtgcagga ggcgctggcg gcccgcccct gggcgttcct
      901 gccgatcacg gcgctggagg cagcgatcac cttgagcacg tgggtacttg ccgggctctt
      961 tatctaccac accgtgcacc agttgcgcac cgtcaccgcc atctatgccg agcatacgcg
     1021 tattaatctg ttcaccctcg gcccgctcta caccttttcg cggctaacgg ctctgactgc
     1081 tatcgcgccg atgccgctgt tcctggttga ttatctgggc tggccggggc agggtaacga
     1141 gccgatcaca accggcacgc tgggcttatg gggtctgttt ggcctgttga gcgtatgcac
     1201 gtttgtgctg ccattgttgg gggtgcgccg gctgctcgct caggagcgcg cgcaggccca
     1261 actcgacctg gccgcgcggc tgcggaccct gctggccggg ctccatcagc aggtgtccag
     1321 cggcgcggcc agtgccgaca gcaccacggc gacgaagaac ctgcttgaca gcctgctagc
     1381 cgaacagggg ctgttgaaca aggtctccag ctggccgtgg gcgccggaaa cgccgcgggc
     1441 cctggccact gccctgctcc tgccggtgct ggtcgtgatc atcagtgagt tggtgaaacg
     1501 cttatttggc ctctagccgc gggtccgcaa ctgggccgtc tccactttct ctccgtcgcc
     1561 actgtggcgc cgtctaccga gctcgggacg tgagcgggcg catcatcggc ctcgacacaa
     1621 tagtatagga acgcatggag gaacgcacaa tggcaactgt aactaacacg ctgcccgtac
     1681 cgaccaccgc cacgcgcatg ggccggcggg tattacgcgg gatcgcccgt ggtgggggcg
     1741 tggtgctggg cctcatcgcc ggcctggcgc tggccggggc cgcctatgaa gccatcgccg
     1801 cgcggggcga tgcgcacacc tatccgccgc cgggccagat ggtcgacgtc ggtggctacc
     1861 gcttgcatat ccactgcgtg ggcacgggca gcccgaccgt ggtgctcgac gccgggctga
     1921 gccgcacctc gctcgactgg agcctggtgc aaccggctat cggccagacg acccgggtgt
     1981 gtgcctatga ccgcgccggc atgggctgga gcgaccctgg cccggcgccg cgcacgcccg
     2041 cgcagattgc cggcgaactg cataccctgc tgggcaacgc gggcctcgcc gggccctacg
     2101 tcctggtcgg gcactcgctg gccggcaaga acgtgcgcct gtttgcgctg gcgcaccccg
     2161 ccgaggtcgc cagcctggtg ctggtggacg cgcggagtga gtatgtggac gcgcacacct
     2221 cgccagccga ggcggcggcc ttccagcagg cgctggcgca gcaggggacg atctacgggc
     2281 tggcgcgcgc cgtgggcctg gtccgtctcg tcggcgcgag ccaggagggc gcgccggagc
     2341 tgccggcgga gacgcgccag gcgatggcgc tgttcagcac ggggcagcgg gggcttgcta
     2401 ccaccgccgc cgaagggcag caacgcgccg ccgacgacgg ccaactccag gcggcgccgg
     2461 ggctgggcgc gcggccgttg atcgtgctgg cggcggggca gaatatggag cagaccccct
     2521 actgggccga cgcccagcgg cagcaagcgg cactgagcac gaacgggcgc ctgatcgtgg
     2581 ccgccgggag cgggcacggt attaacctgg agcggcccgc cctggtcatc gacgcggtac
     2641 ggcaggtggt caagcaggtc cggggtcact aagccccgga cctgcttgag cgcccaccgg
     2701 tgccggtccg ttcacggcaa ggccacgaat cagcagtggc aatccgccct acaagcactt
     2761 tactgaggag gcactcttat ggcactagca acctggtggc gcggcgatgc cctgcccacc
     2821 ctggatcccc tgcccgactt tcacgtcgaa gcatcgcgtg acgaacgcct gattgcccgc
     2881 gtcaccgggt tggatcccga acaggtgcgg gagcgcctcg ccgggggcca ccaaccgtac
     2941 ctggcctcca tcgccacggc accggccagc tatggttggc tggcgcagca ggaagccgag
     3001 attggcgaac tgggcttgcg ctttgcgctg ccgccggtcg accgctacct gtgggatttc
     3061 gccacgctgc cggcctggca ggggtacggt atctacccgc ggctgttgca gagcattctt
     3121 cggcagcaag ccctgccggt cgaacggatg tggataatct atgctccgga gaacacgccg
     3181 tcgggggcgg ggatgcacaa agcgggcctg ctgcctgtcg gccacctctc ctttcggagc
     3241 gacgggacag tcgaattagc cgccagcggc gcgccgcaac gcgcgcaggt cggggcggac
     3301 ctgctcggcg tgccgatcag cgagagcgtg ctggcgccct gttgggcttg tggccgcgtg
     3361 acatccccgc ctgccacgca cgtggacgcc acaggctgct ggcccccgcg gccgtcgacg
     3421 gccacacgct gtacctgcgc cgtggaactg caaccggcca aaacgatcgc caccgccggc
     3481 gcggaaaatc aatagtcgcg ggctccgccg gccattgctt accatactca cggagtaggt
     3541 aaggatggcc ggcgctaatg cgttgttccc tgttgcaggt cggtgactga tggtgctccc
     3601 gctggcctgc gcatccgcgg cgctagaccc ctagacgctc caggacgcgc tgcacaaacc
     3661 agaagatcat cggcacgaaa aaggccacgc cgaagccggc cactgtctcc gtccgccagg
     3721 gccaggtgcg cagcttgtcg atcatacctt gttcggtcac cagtccgtcg agcacggcct
     3781 tcaacgcggc gaattcggtc agatcgccgg tatccgcccg ccggtgcagc gt
//
LOCUS       DASUYC010000716         2937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1495717, whole genome shotgun sequence.
ACCESSION   DASUYC010000716 DASUYC010000000
VERSION     DASUYC010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1495717"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="VF276_11695"
     CDS             complement(<1..839)
                     /locus_tag="VF276_11695"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF014553.4"
                     /note="catalyzes the reversible transfer of the terminal
                     phosphate of ATP to form a long chain polyphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HEX6607566.1"
                     /translation="MAVEEIKTEQSAPALVFQQPVLDARTYINRELSLIEYQKRLLDE
                     VADERNPLLERVKLFAIVQANLDEFFMVRVSGIREQVQAGVIEKSPDGMTPAEELAAI
                     REALIRLIPVMHDLFESRLRPALAEAGIVIAEYADLAPEQREALCDYFERMVFPVTTP
                     LAVDTGHPFPHISNLSLNLAVELEDPEGRRYFARVKVPSVLPRLVPLPSNDGLDGRGQ
                     PAVYVWLDQLLAANLDALFPQMHLLEIHPFRVIRDADLEIQELEADDLLVTVERSLSR
                     RRFG"
     gene            complement(830..1756)
                     /locus_tag="VF276_11700"
     CDS             complement(830..1756)
                     /locus_tag="VF276_11700"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607567.1"
                     /translation="MSRTWAVADIGSNTVHLLVARVAGDALAPLVDQTANLRLGGEVD
                     RDGAIGPAKVDELIATLRGFQAAALAAGAARLYCFATEAMRAATNGAAVLSAVTAATG
                     LPVHLLPPEAEAGLGYRGATAGVPGAGLRAVVDIGGGSLQVIVGEGEAIRGGVSLPLG
                     AARLVARFLPDDPPEAGDAARLAAHLAAVIPPALPLRNQDITALLGTGGTLRRLAAVR
                     GWDAGRPLPADALDAALTWLRDLPAARLAAEADLPVDRARMFLPALLAWREVWRGYHA
                     PPLWVVPGGVREGAILHLARAGLNERGELRWP"
     gene            2238..>2937
                     /locus_tag="VF276_11705"
     CDS             2238..>2937
                     /locus_tag="VF276_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607568.1"
                     /translation="MFAHTTMGSINWGAFARGLAVFSIGLSLMGVLTVPAATLPDRAL
                     PPDPVQSRAAPDGSFQQSALPQPIPTEPVGTIFHAAGPAEGGGFSAYLDGNITTVALQ
                     VRRFNGAAEAHPLRQTVLFYVSYSDLTHTYNCAYGPIPDGDLIGSGAGPLHLKTDTSA
                     NKEPDFYRCGPGGRIDVLLQGDQMMQHRASGDDEMAYGNLVYRSSQQATEWSALVSGS
                     VYGMPLPVTEGTLTH"
BASE COUNT          439 a         1075 c          982 g          441 t
ORIGIN      
        1 ccaaagcgcc gccgcgacag gctcctttcg accgtcacga gcaggtcgtc ggcctccagc
       61 tcctggatct ccaggtcggc gtcgcggatg acgcggaagg ggtggatttc gagcaggtgc
      121 atctgcggga agagggcgtc gaggttggcg gccagcaact ggtcgagcca gacgtagacg
      181 gcgggctggc cgcgcccgtc gaggccgtcg ttgctgggca gcggcaccag gcgcggcagc
      241 acgctgggca ccttgacccg cgcgaaatag cgccgcccct ccgggtcctc cagttccacg
      301 gccaggttca ggctcaggtt ggagatgtgc gggaaggggt ggccggtgtc cacggccagc
      361 ggcgtcgtga ccgggaagac catgcgctcg aaatagtcgc acagggcctc gcgctgctcc
      421 ggcgccaggt cggcgtactc ggcgatgacg atgcccgcct cggccagcgc ggggcgcagc
      481 cggctctcga acaggtcatg catcaccggg atcagccgga tgagcgcctc gcggatggcg
      541 gccagttcct cggcgggggt catgccgtcg gggcttttct cgatcacccc ggcctggacc
      601 tgctcgcgga tgcccgacac gcgcaccatg aagaactcat ccaggttggc ctggacgatg
      661 gcgaacagtt tgacgcgctc cagcaggggg ttgcgctcgt cggcgacctc gtccagcagg
      721 cgcttctggt actcgatcag gctcaactcg cggttgatgt aggtccgcgc gtcgaggacc
      781 ggctgctgaa agacgagggc gggcgcggac tgctcggtct tgatctcctc tacggccatc
      841 gtagctcacc tctttcgttc agcccggcgc gcgccaggtg caagatcgcg ccctcgcgca
      901 cgccgcccgg caccacccac agcggtgggg cgtggtagcc gcgccagacc tcgcgccagg
      961 ccagcagggc cgggaggaac atccgcgccc ggtccacggg cagatcagcc tcagccgcca
     1021 gccgcgcggc gggcaggtcg cgcagccagg tcagggccgc gtccagtgca tcggcgggca
     1081 ggggccgccc ggcatcccag ccccgcaccg cggccagccg gcgcagggtg ccgcccgtgc
     1141 ccagcagcgc tgttatatct tgattacgta gcggcagcgc gggcgggatc accgcggcga
     1201 ggtgggcggc cagccgggcc gcgtccccgg cctctggggg atcatccggc agaaaccggg
     1261 ccaccaggcg cgccgccccc agcggcaacg acacgccgcc ccggatcgcc tcgccctcgc
     1321 ccacgatgac ttgcaggctg cccccgccga tgtcgaccac ggcgcgcagg cccgcgcccg
     1381 gcacccccgc cgttgccccg cgatagccca gcccggcctc ggcttccggc ggcagcaggt
     1441 gcaccggcag cccggtcgcc gccgtcacgg cgctcagcac ggcggcgccg ttcgtggcgg
     1501 cgcgcatggc ctcggtggcg aagcagtaga gcctcgccgc gcccgcggcc agggccgcgg
     1561 cctggaaccc gcgcagggtc gcgatcagtt cgtccacctt ggccgggccg atggccccgt
     1621 cgcgatcgac ctcgccgccc aggcgcagat tggccgtctg atcgaccagc ggcgccaggg
     1681 cgtccccggc cacgcgggcc accagcaggt gcaccgtgtt gctgccgata tcggccaccg
     1741 cccaggtccg gctcacactt gccctcccgc cgcgcccagc ggccccggcg cgcgccgctg
     1801 tgtccggttc atcatatcac acgttgcgcg tgccgcgcta gacgcggcgc ccgcccgctg
     1861 gtctgggcgc ggtgcgcggg ttctgcctta ccggcgcggc acaattatgg tcgcctattt
     1921 agctgatgtt gaaaattggc acttgacttt ccggcgggcc ggtgctagaa tatactctat
     1981 acaaatctcc tcgtagttgg gtcctcttgt ccatcgcccc cggcgtgagg cgagtatatg
     2041 cgccgcggtc cgtatcaagg aggcgcaaca tggtccatca ctcctaaccg caagccgtag
     2101 ccattctacc aatctaactc cacctaccgt tcgcccggcc tgccctcctg gcagcgcccg
     2161 cctgggggcg ccgtgtcccc tggcgcccgc ctgccggccg ggaagtctcg ttagcggccc
     2221 actaaaggag caccacgatg tttgcgcata ccaccatggg gtccatcaac tggggcgcgt
     2281 tcgctcgggg cctggctgtg ttcagtatcg gtttgagtct gatgggcgtg ctgaccgtgc
     2341 ccgcggccac gctcccggat cgcgccctgc cgccggaccc ggtccagagc cgcgccgccc
     2401 cggacggcag tttccagcag agcgccctac cgcaaccgat cccgaccgag ccggtaggca
     2461 ccattttcca cgccgccggt cccgctgaag gcggcggatt ctccgcgtat ctcgacggca
     2521 acatcaccac cgtcgccctg caggtccggc gcttcaacgg cgctgccgag gcgcatcccc
     2581 tgcggcagac ggtgttgttt tacgtcagct acagcgactt aacccacacc tacaattgcg
     2641 cctacgggcc gatccccgac ggggatctga tcggcagcgg ggccgggccc ctccacctca
     2701 agacggacac cagcgcgaac aaggagcccg atttctatcg ctgcggcccc ggcggccgga
     2761 tcgacgtact gttgcagggc gaccagatga tgcagcaccg ggccagtggc gacgacgaaa
     2821 tggcctacgg gaatctcgtg taccgcagca gccagcaggc caccgaatgg tccgcgctgg
     2881 tcagcggcag cgtgtacggc atgccactgc ccgtcaccga aggcacgctc acgcacg
//
LOCUS       DASUYC010000717         3008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_802932,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000717 DASUYC010000000
VERSION     DASUYC010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802932"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1934
                     /locus_tag="VF276_11710"
     CDS             <1..1934
                     /locus_tag="VF276_11710"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEX6607569.1"
                     /translation="GLTPVARAADQNQYWDNDEVTVQIHEDSTFDVTERQGFVFDSGT
                     FHGSYRDIETNRLTGITNVSVSEDGVGAYQTDNTPIDLKAGKYGPPRTFQVLDQSGSK
                     RIIWFYGTLDAPAHKTMVIKYTVSGGLRFYPDGDQFYWSPFMPNRTAVIERASITVQV
                     PPNIDLTTGPARQWAVYPANAATVQVSPQQAVFTVKGALQPNTDFEAGLQWAHGAIAG
                     TAAPWQIAADQGHDRQGWATLIVVVLSLLVGIGGPLFWLGRWYGWGRDPVSGPVAGNL
                     PAPPSDLPAGLVGTLVDEQAKPRDVIAAVLDLGRQGHLRIEQVKPRDVIGVPRAWTDF
                     SYTLLNRDVRYTHERLVLDLLFGQKLTEIRLSQLSHFHLKLPQVYSAMYQDLLDLKYF
                     TSRPDTTRNRYNQAALWLLGLAVLSGVFTPLGLALTPMFWLLPVACAVAAGAGFIAAG
                     KMPRKTVRGADQAARWRAFGRYLGDLQQFGDLGNAAQRFGDYLPYAVALGLGGKYIDQ
                     FNAAQTPPALPTYYQPRNRLDTVDIPNVNRSSRAGAGLGNGISIGGGSSSGGRGLDLS
                     PSGANALNQGIALSLTNFNTNLTGMINNTSNVLGLGHPLGPGPGDGADTVGAVLNVAG
                     AVLDVVAGGGGGGGGGGSF"
     gene            2166..>3008
                     /locus_tag="VF276_11715"
     CDS             2166..>3008
                     /locus_tag="VF276_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607570.1"
                     /translation="MTNVPDFRGRVESNKPQDFFHRLAEKIPGYTGYQDRERRREADK
                     ELRMYLARGFRDQYAALQRVQQQMARSRHLESIGEVDRLSGMLQRFIDKLETATQGYA
                     GLFDPVKVDMPQLDQLYTFDAALTDELQKVSDNIAAVSAASQQGTGPAPGATAASAPD
                     LPTALSALSATLDELLRTWNHRNEVIMSGQPMPAQEFERFRASVGADTPQPYTNATGG
                     TGNAPAQAGAQGTQGYPAPPPAPQQGPGQPYQPYAGPQQGSGQPAYSGPQPGGGPPTY
                     PAQQP"
BASE COUNT          521 a         1026 c         1018 g          443 t
ORIGIN      
        1 tgggcctcac cccggtggcg cgcgccgcgg accagaacca atactgggat aacgacgaag
       61 tcaccgtcca gatccatgag gactcgacgt tcgacgtgac cgagcggcag ggctttgtct
      121 tcgacagcgg cacctttcac ggtagctacc gtgatatcga gaccaaccgg ctgaccggca
      181 tcaccaacgt cagcgtcagc gaagacggcg tgggggctta ccagaccgat aatacgccta
      241 tcgacttaaa agcgggtaaa tacggcccgc cgcggacctt ccaggtgctc gaccagagcg
      301 gatcgaagcg tatcatctgg ttctacggca cgctggacgc gcccgcgcac aagacgatgg
      361 tcatcaagta taccgtgagc ggtgggctgc gcttctaccc ggacggcgac cagttctact
      421 ggagcccgtt catgcccaac cgcacggcgg tcatcgaacg cgcgagcatc accgtgcagg
      481 tgccgccaaa catcgatctg accacgggtc cggcccgcca atgggccgtc tatcccgcca
      541 acgcggccac ggtgcaggtc agcccgcagc aagccgtgtt cacggtgaag ggggcgctcc
      601 agcccaatac ggactttgaa gccgggttgc aatgggcgca tggcgcgatc gccgggacgg
      661 cggcgccctg gcagatcgcc gcggaccagg ggcacgaccg gcaagggtgg gccacgctga
      721 tcgtcgttgt cttgagtctg ctggtgggta tcggcgggcc gctgttctgg ctgggccgct
      781 ggtacggctg gggtcgcgat cccgtgagcg gcccggtcgc cggcaacctg cccgcgccgc
      841 catccgatct gcccgccggg ctggtcggca ccctggtcga cgagcaggcc aagccgcgcg
      901 atgtcatcgc cgcggtcctg gatctgggac ggcaggggca cttgcgcatc gagcaggtta
      961 agccgcgcga tgtcattggg gtgccccgcg cgtggaccga cttcagctac acgctcttga
     1021 accgcgatgt gcgctacacc catgaacggc tggtgctcga tttgctgttc gggcagaagc
     1081 tgacagagat ccggctgtcg caactgtcgc actttcacct gaagctgccc caggtctaca
     1141 gcgcgatgta ccaggacctg ctcgatctga agtacttcac gagccggccc gacacgacac
     1201 ggaatcgcta taaccaggca gcgctgtggc tgctggggct ggccgtgtta agcggtgtgt
     1261 tcacacccct cggcctggcg ctgacgccga tgttctggct gctgccggtg gcctgcgccg
     1321 tggcggcggg cgccggcttt atcgcggcgg gcaagatgcc gcgcaaaacc gtgcgcggcg
     1381 ccgaccaggc ggcccgctgg cgcgcctttg gccgttacct gggcgacctg cagcagttcg
     1441 gcgacctggg taacgcggcg caacgcttcg gcgactatct gccctatgcc gtcgcgctgg
     1501 gcctcggcgg gaaatacatc gaccagttca acgccgctca gacgcccccg gccctgccca
     1561 catattacca gccgcgcaac cggctggaca cggtcgacat tcccaacgtg aatcgtagca
     1621 gcagggccgg cgcggggctg ggcaacggga tcagcatcgg gggcggcagc agcagcggcg
     1681 ggcgggggct cgacctatcg cccagcggcg ccaacgcgct caaccagggt atcgcgctct
     1741 cgctcaccaa cttcaacacc aacctgaccg ggatgatcaa caataccagc aacgtgctgg
     1801 ggttgggtca ccctttgggg ccagggcccg gcgacggggc cgacacggtg ggggccgtgc
     1861 tcaacgtggc cggtgcggtg ctcgacgtgg tggccggcgg tggtgggggc ggtggcggcg
     1921 gcggctcctt ctagcgcccg gcctggagtg atggcactct gcgtgctggg ataaggcacg
     1981 cagcccggcc tggcagggta gggggcgcct gcgcccgcgg gccgtgaaac acgaatccgg
     2041 gcgcccgcgt agatcgaggg cgagtcaacc tgatccggaa agcgacgtgt agaccaccgg
     2101 ctgcacggcc gggttatcga ttggggcacg cccgaggcgt gtcgcgtgta gaggaggaac
     2161 ggcacatgac gaatgttcct gatttccgcg gacgagtcga gagtaataaa ccgcaggact
     2221 tcttccaccg gctggcggag aagatccccg gctacaccgg ctaccaggac cgcgaacgcc
     2281 gccgcgaggc cgacaaagag ttgcgcatgt acctggcccg cggcttccgc gaccagtatg
     2341 cggcgctcca gcgcgtgcag cagcagatgg cgcgcagccg ccacctggag tccatcggcg
     2401 aggtggaccg cctgtcgggg atgctgcagc gctttatcga caagctcgaa acggccacgc
     2461 agggctacgc cgggctgttc gatccggtca aggtcgatat gccgcaactc gatcaactct
     2521 ataccttcga cgcggcgctg accgacgagt tgcagaaggt ctcagacaat atcgccgccg
     2581 tctcggccgc atcccagcag ggcaccggcc cggcccccgg cgccacggcg gcgagtgcgc
     2641 ccgacctccc gacggccctc agcgcgctga gcgccacgct ggacgagttg ctgcgcacct
     2701 ggaaccaccg caacgaggtg atcatgtcgg gccagccgat gcccgcgcag gagttcgagc
     2761 gcttccgggc cagcgtcggc gccgatacgc cgcagccgta caccaacgcg acgggcggaa
     2821 ccggcaatgc gcccgcgcag gccggcgccc agggcacgca aggctatccc gcgcccccgc
     2881 ccgcgccgca acaaggcccc ggccagccct accagcccta tgccgggccg caacagggca
     2941 gcggccagcc ggcctactcc ggcccgcagc cgggcggcgg cccgcccacc tatccggccc
     3001 agcagccg
//
LOCUS       DASUYC010000718         2509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1477004, whole genome shotgun sequence.
ACCESSION   DASUYC010000718 DASUYC010000000
VERSION     DASUYC010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1477004"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="VF276_11720"
     CDS             <1..976
                     /locus_tag="VF276_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HEX6607571.1"
                     /translation="MPLPGAYPLSLPADAALPAERAAVGTDLLVGDAAQRSGVNGLEV
                     AEDAKIVCFPDLMALYQSGRLNMEGVQAVQQAMLDHAQNMGNRVAILDSPPGMTPQQI
                     RDWRMKEVNYDSKYGALYYPWIKVADPVSGGSIMVPPCGHMAGIWSRSDSQRGVHKAP
                     ANEVIAGCIGLEYQTTSSEQAILNPEGINVIRSFPGRGIRVWGARTLSSDPAWRYLNV
                     RRLFNYVEESLQRGTQWVVFEPNDMDLWERVKRDISAFLVRTWRDGALFGNTASEAFY
                     VKCDAENNPVEVRDVGQLIVEIGIAPVKPAEFVIIRLSQWQGGGSASE"
     gene            1006..1470
                     /locus_tag="VF276_11725"
     CDS             1006..1470
                     /locus_tag="VF276_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012692663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEX6607572.1"
                     /translation="MPGMDGGGVRKDPLVQANFFVSVDGHLDNMVFRECSGLGSETEL
                     VEYKASMKGDFHTIQAVPGRLKWQVINLKRGITDSMDAWKWRKMVEEGQVDQARANGS
                     IVMVDQTGAEVARWNFIRAWPRKISGPSMNSSTNEVGVEELEIVHEKLTRVK"
     gene            1562..1960
                     /locus_tag="VF276_11730"
     CDS             1562..1960
                     /locus_tag="VF276_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020620952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HEX6607573.1"
                     /translation="MVGTNSKPRAEKGLQTEYEFTLPRGYVDSDGNLHRHGVMRLATA
                     MDEITPMRDMRVKQNQAYLTVVLLSKVITKLGTLEEVHTGVIEKLFSADFAYLQDFYR
                     RINEAEHVTIPAQCPHCNHEFEVELVPSGG"
     gene            1957..2118
                     /locus_tag="VF276_11735"
     CDS             1957..2118
                     /locus_tag="VF276_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015324981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6760 family protein"
                     /protein_id="HEX6607574.1"
                     /translation="MTGYPPDQLREEVAYIAYYFHWPRQEILELEHHERRAWVEQIAG
                     INRQLNVEE"
     gene            2135..>2509
                     /locus_tag="VF276_11740"
     CDS             2135..>2509
                     /locus_tag="VF276_11740"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX6607575.1"
                     /translation="MDDTEREPDSAFAGNSPERAGAGPWVSRLIGRSLSDVRVHDTSQ
                     AGDLAARLGARAFTAGRHVYVPPARVHPLTPEGVALLAHEMTHAVEQTGAAPVAPLPL
                     SAPAGRLAPVQRVPESGGEARAV"
BASE COUNT          453 a          830 c          841 g          385 t
ORIGIN      
        1 catgcccctg ccgggcgcct atccgctcag cctgcccgcc gatgccgccc tgcccgccga
       61 gcgcgccgcc gtcggcaccg acctgctggt cggcgatgcc gcgcagcgca gcggcgtcaa
      121 cggcctggaa gtagccgaag atgccaagat cgtctgcttc cccgacctca tggcccttta
      181 tcagtcgggc cgcctcaaca tggaaggcgt ccaggcggtg cagcaggcca tgctggacca
      241 cgcccagaac atgggcaacc gcgtcgccat cctcgacagc ccgccgggta tgaccccgca
      301 gcagatccgc gactggcgca tgaaggaagt caactacgac tccaagtacg gcgccctcta
      361 ctatccgtgg atcaaggtgg ccgacccggt cagcggcggc agcatcatgg tgcctccgtg
      421 cggccacatg gccggcatct ggtcgcgtag cgactcgcag cgtggcgtgc acaaggcccc
      481 ggccaacgag gtcatcgccg gctgcatcgg cctcgaatac cagaccacct cctcggaaca
      541 ggccatcctg aacccggagg gcatcaacgt catccgcagc ttccccggtc gcggcatccg
      601 cgtctggggt gcgcgcactc tctccagcga cccggcgtgg cgctacctga acgtgcgccg
      661 gctgtttaac tatgtggaag agtcgttgca gcgcgggacg cagtgggtgg tcttcgagcc
      721 gaacgacatg gacctctggg agcgcgtcaa gcgtgacatc tcggccttcc tggtgcgcac
      781 ctggcgcgac ggcgcgctct tcggcaatac cgcgagcgag gccttctacg tcaagtgtga
      841 cgccgagaac aatccggtcg aggtgcgcga tgtcggccaa ctgatcgtcg aaatcgggat
      901 cgcgccggtg aagcctgccg agttcgtgat tatccgcctg agccagtggc agggcggcgg
      961 gtcggccagc gagtaagcag cctactacaa gggaggtaac gcacaatgcc cggtatggat
     1021 ggtggtggcg tccgtaagga ccccctagta caagctaact tctttgtcag cgtggacggc
     1081 cacctggaca acatggtgtt ccgcgagtgc agcggcctcg gctccgaaac cgagctggtc
     1141 gagtacaaag cctcgatgaa gggcgacttc cacacgatcc aggcggtgcc cggccgcctc
     1201 aagtggcagg tcatcaacct caagcgcggc atcaccgact cgatggatgc ctggaagtgg
     1261 cgcaagatgg tcgaggaagg ccaggtcgat caggcccgcg ccaacggctc gatcgtcatg
     1321 gtggaccaga ccggcgccga agtggcccgc tggaacttca tccgcgcctg gccgcgcaag
     1381 atcagcggcc cctcgatgaa ctcgtcgacc aacgaggtgg gcgtcgagga gctggagatc
     1441 gtgcacgaga agctgacccg cgtcaagtag acccggcgtt ccgcgattct gcggggcaag
     1501 gggggcaccc cccggcgggt ggtccccttg ccccagccct gttcattgat gggagccaag
     1561 tatggtcggc acgaacagca aaccccgcgc cgaaaagggg ttgcagaccg aatacgaatt
     1621 caccttgccg cgcggctatg tcgatagcga cggcaacctg caccgccacg gcgtgatgcg
     1681 cctggcgacg gccatggacg agatcacgcc gatgcgcgac atgcgcgtga agcagaacca
     1741 ggcgtatctg accgtggtgc tgctctcgaa ggtgatcacc aaactgggca cgctcgaaga
     1801 ggtgcatacc ggcgtgatcg agaagctgtt ttccgccgat ttcgcctacc tgcaggactt
     1861 ctaccggcgc atcaacgagg cggagcatgt cacgattccg gcccagtgcc cccactgcaa
     1921 ccacgagttt gaagtggagt tggtgccctc ggggggataa ccggctaccc tcctgatcag
     1981 ctccgggaag aggtagccta tatcgcctat tatttccact ggccgcgcca ggagatctta
     2041 gagctggagc accacgagcg gcgggcgtgg gtcgagcaga tcgccgggat caaccggcag
     2101 cttaacgtcg aagagtagcg gagcaccggc ggcgatggac gacacggaac gcgaaccgga
     2161 tagcgccttc gcgggcaact cgcccgagcg ggccggggcc ggcccctggg tgagccggct
     2221 gatcggccgg tcgctgagcg atgtgcgcgt ccacgacaca agtcaggccg gcgatctcgc
     2281 cgcgcggctg ggggcgcggg ccttcaccgc cgggcgccat gtctacgtgc cgcccgcgcg
     2341 cgtccacccg ctgacgcccg agggcgtggc gctgctggcg cacgagatga cccacgctgt
     2401 ggagcagacc ggggcggctc cggtggcccc gctgccgctc tcggcgcccg ccggccggct
     2461 cgcgccggtg cagcgggtcc cggaatcggg gggcgaggcg cgggccgtg
//
LOCUS       DASUYC010000719         2488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_930376,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000719 DASUYC010000000
VERSION     DASUYC010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930376"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VF276_11745"
     CDS             <1..335
                     /locus_tag="VF276_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607576.1"
                     /translation="LAYEALAPGGTLWLREVRAGLARVLRPAAGGAPGSLVEATLARL
                     GAHAGLIDDLPALLAAAGFVRVQPVPEAYSPEGTPRSLRRLPGVLLRPPAAEASVPAT
                     NFIARRPR"
     gene            471..1322
                     /gene="tsaA"
                     /locus_tag="VF276_11750"
     CDS             471..1322
                     /gene="tsaA"
                     /locus_tag="VF276_11750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEX6607577.1"
                     /translation="MDGDEQAAPVLLRPIGVVRSPVRERGAMPGAGVPATVEVFPAYR
                     AGLVDIASNSHLIVLAWLHLAERDRLQAAGRHAPPGAPLRGVFGLRSQMRPNPIGCTV
                     ARLLAADLPAGRLDLDRLDFLDGTPVLDLKRYSPAIDAVFAARTSRDFATPPPDLRDD
                     LARAAANFHGEWCAGAAVGVALLAAALVAWGCAARHPDLTVQVGADGCLADALQGMTG
                     ATLGNRRLTVAPGPTVRLRYPPHTWTFTPAYDPAAAPPDALLAQPLAALGLLETLDDG
                     DPSLRSE"
     gene            1621..>2488
                     /locus_tag="VF276_11755"
     CDS             1621..>2488
                     /locus_tag="VF276_11755"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX6607578.1"
                     /translation="MADSTTERPDAPAAVPPDPYAPFYIWISGTADAPQVAVDHAPGG
                     DLAQSALDVRYLPAVQRLVGLVHGGATDPGTVANLGRGLYTILFTPALRDLYQRSLGR
                     LAAGGRLRLLLRVDPPALAALPWELLYDPTDNIFLALDPVVSVARYLPQAARPAPAPR
                     SGPLRVLAVFASPTTLPALDLTRELALIVTALQTLPPGQVALDVLMDAPTAAVAQLGI
                     VPKGPPTPAAVTDAIGAGYDVLHYGGHGALQDGGGLLALENADRSAAFFDAASLGIAV
                     KGTRLRLAVLTAC"
BASE COUNT          306 a         1012 c          846 g          324 t
ORIGIN      
        1 acctggccta tgaggcgctg gcgcccggcg gcacgctctg gctgcgcgag gtgcgcgccg
       61 ggctcgcgcg cgtgctgcgg cccgccgccg ggggcgcgcc gggcagcctg gtcgaggcca
      121 cgctggcccg cctgggcgcc cacgccgggc tgatcgacga tctgccggcg ctgctggccg
      181 ccgccggatt cgtgcgcgtg cagcccgtac ccgaagccta ctcgcccgag gggacgccgc
      241 gcagcctgcg ccgcctgccg ggcgtcctgc tgcgcccgcc tgccgccgag gccagcgtgc
      301 ccgccaccaa ctttatcgcc cgccgcccgc ggtagtactg ccttcagtga tccgtgtcac
      361 tctgagcgcc gcgaagagtc tcgtcgggga accagacgag atgcttcact gcgttcagca
      421 tgacaattaa gacggcgagc cacacccggc aggcggcggc aggaggcgcg atggacgggg
      481 atgagcaggc cgcgccggtg ctgctgcggc ccatcggcgt tgtgcgcagc cccgtgcgcg
      541 agcgcggcgc catgccgggc gccggcgtgc ccgccacggt cgaggtcttc cccgcctacc
      601 gcgccgggct ggtggacatc gccagcaaca gccacctgat cgtgctggcc tggttgcacc
      661 tggccgagcg cgaccggctg caggccgcgg ggcgccacgc gccgccgggc gcccccctgc
      721 gtggtgtgtt cggcctgcgc tcgcagatgc gtcccaaccc catcggctgc accgtggccc
      781 gcctgctcgc cgccgacctg cccgccgggc ggctcgacct cgaccggctc gacttcctcg
      841 acggcacgcc cgtgctcgac ctcaagcgct actcccccgc catcgacgcc gtgttcgccg
      901 cacgcaccag ccgcgacttc gccaccccgc cgcccgacct gcgcgacgac ctggcgcgcg
      961 ccgccgccaa cttccacggc gagtggtgcg ccggggccgc cgtgggcgtg gcgctgctgg
     1021 ccgcggccct cgtggcctgg ggctgcgccg cccgccaccc cgatctaacg gtgcaggtgg
     1081 gcgccgacgg ctgcctggct gacgccctgc aaggcatgac cggcgccacg cttggcaacc
     1141 gccgcctgac cgtcgccccc ggccccaccg tccgcctgcg ctacccaccc cacacctgga
     1201 ccttcacccc cgcctacgat cccgctgccg cgccgcccga cgccctgctc gcccagcccc
     1261 tcgccgccct cggcctgctg gagacgctcg acgacggaga cccttcgctg cgctccgagt
     1321 gacacggcgc acacccgatt acggccaagc atacatctgt gaccaccacg cacccccacc
     1381 tttgtctggc gggatatgta tcgcgctatg agcgggcatc cgggtgggcg acaaacctaa
     1441 cccccctggc cccccttccc gccacgggaa ggggggaatc gcgggtgcaa gaaaaagtat
     1501 ctcccctccc cgcggcgggg ggggagaggt tgccccccgc gccgggatgt tgacaacccg
     1561 cgatccgccg ggtaagatag ccgcacaagc gccgtcaccc ccttcaacag gagcagagcc
     1621 gtggccgaca gcacgacaga acgccccgat gccccggcgg cggtgccgcc cgatccctac
     1681 gcaccgttct atatctggat cagcggcacg gcggacgcgc cgcaggtggc cgtggaccat
     1741 gcgccgggcg gcgacctggc gcagagcgcg ctcgacgtgc gctacctgcc cgctgtgcag
     1801 cggctggtcg gactggtgca cggcggggcc accgaccccg gcaccgtggc gaacctgggc
     1861 cgcgggctgt acaccatcct gttcacgccc gccctgcgcg acctctacca gcgcagcctg
     1921 ggccggctgg cggcgggcgg gcggctgcgc ctgttgctgc gggtggaccc ccccgcgctg
     1981 gccgccctgc cctgggaact gctctacgac ccgaccgaca acatcttcct ggcgctcgac
     2041 ccggtggtgt cggtggcgcg ctacctgccg caggccgcgc gacccgcgcc cgcgccgcgc
     2101 tcggggccgc tgcgcgtgct ggccgtgttc gccagcccca ccaccctgcc cgcgctcgac
     2161 ctgactcgcg aactggccct gatcgtcacg gcgttgcaga cgctgccgcc gggccaggtg
     2221 gcgctcgacg tgctgatgga cgcgcccacc gccgcggtgg cccaactggg catcgtgccc
     2281 aaggggccgc ccacccccgc cgccgtcacc gacgccatcg gcgccgggta tgacgtgctg
     2341 cactacggcg ggcacggcgc gctgcaggac ggcggcgggc tgctggccct ggagaacgcc
     2401 gaccgcagcg cggccttctt cgacgcggcc agcctgggca tcgccgtgaa gggcacgcgc
     2461 ctgcgcctgg ccgtgctgac cgcctgcc
//
LOCUS       DASUYC010000720         2922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_812497,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000720 DASUYC010000000
VERSION     DASUYC010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812497"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VF276_11760"
     CDS             complement(<1..294)
                     /locus_tag="VF276_11760"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HEX6607579.1"
                     /translation="MPDVSPIDPCREPPPLALYTGIAEFNRGEYFECHETLEAIWMRE
                     PRPIRRLYQGILQVGVGFYHLRNGNYRGTLNLLGSALGYLADFTPACMGVDVAR"
     gene            395..661
                     /locus_tag="VF276_11765"
     CDS             395..661
                     /locus_tag="VF276_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607580.1"
                     /translation="MTYWKPVLGRLALSTASLAIIAGLGGFMGAILGAVFELMLQAIL
                     GLALEPLTVIPLFMILGLVLSLIWGLSLLVQHRAPASVTPPAGQ"
     gene            978..1484
                     /locus_tag="VF276_11770"
     CDS             978..1484
                     /locus_tag="VF276_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607581.1"
                     /translation="MPVREWHFTLEDGRHTVVYETSTFGGKRTITVDNVPRVEDAGRA
                     WQMGADIPFRINQHTGAVHVRSGMLRTQYDLSIDGVSVESGRPMVSLLPLPGWAWIFI
                     GACALIPIITLGGAVPAGIGAGGAFGCASVARDPNRAASHRVGICTGIAAGCWGLLLG
                     FLALVAGA"
     gene            complement(1566..2756)
                     /locus_tag="VF276_11775"
     CDS             complement(1566..2756)
                     /locus_tag="VF276_11775"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003334205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX6607582.1"
                     /translation="MANDDHPVILGAVRTPFGKFGGSLMSVGAVDLGACVIGEAIRRA
                     DIPKEDVTHNIMGCVVQAGLGQVPSRQAAFKAGLGREVTSDTINRVCASGMRAVALAG
                     DLIRLDRHQVIVAGGMESMSNAPYYLRGARWGQRMGNGELIDGVVYDGLTDPLLNVHM
                     GQHGSTVAAEEKVGREEQDAWAYRSQMRAAAAQDNGIFAQEIVPVPVPQKKGTPVAVD
                     QDEAVRRDTTLEGLAKLKPVFSPTGTITAGNAPGLNDGAGALVVASAAYAKAHGLKPL
                     ARILGYGEAAWDTPYLAYTPAMAAQNALKRLGLTIQDIDLVEINEAFASVAIISARRL
                     GVDPEIVNVNGGAIALGHPIGASGARLLTTLIYELHRRGGGLGLAAICSGTAQGDAVV
                     LQVD"
BASE COUNT          434 a          973 c          980 g          535 t
ORIGIN      
        1 gcgggccacg tccacgccca tgcaggccgg ggtgaagtcg gccagatagc cgagcgccga
       61 accgagcagg ttgagcgtcc cgcggtaatt gccgttgcgc aggtggtaga agccgacccc
      121 gacctgcagg atgccctggt agagccggcg aataggccgg ggctcacgca tccagatcgc
      181 ttcgagtgtt tcgtggcact cgaagtactc gccgcggtta aattcggcga ttccggtata
      241 gagcgccagg ggcggcggct cccggcatgg gtcgattggc gatacatctg gcatagtact
      301 tgctttagcg aaaacctaga acctgggcgc gcaatcacag gtctacaggg accgatcatc
      361 tggtggtggt gccgaccacc tgaaggagtt cgtaatgacg tattggaagc ccgttttggg
      421 gcgtctagcg ctctcgactg cctcgttagc cattatcgcc ggcctgggtg ggttcatggg
      481 ggccatcctg ggcgcggtgt ttgaattgat gttgcaggcc attctcggcc tggccctcga
      541 accgctcacg gtcattcccc tcttcatgat cctcggactg gtgctgagcc tgatctgggg
      601 actgtcgctg ctggtgcagc accgcgcgcc ggcctctgtg acgccgccgg cgggccagta
      661 accccccaca caatccgcac acccttggct cctggtatta gcccaggagc tttttgttac
      721 cgcgggccga acgccccttg acgcagccgc ttcccgatgc tatactacgc ccgctgacgc
      781 acccccggtt acaggcaggc gccgggccat ccgtttcttc taacgttcca gggctcctgt
      841 tgctgcgcac catcctgcat caacatcgct ataccctcgc acctgccgcc gcgggccggg
      901 tcccgatagg cgatccgacc cggcctgccc ggcgccaacc ccatgccctg agatatagcc
      961 aagaggatac tacgttcatg cccgtcagag aatggcactt tacgctggaa gacggtcgcc
     1021 acaccgtggt atatgaaacc agcacgtttg gcggcaagcg cacgataacc gtggataatg
     1081 taccccgcgt ggaagatgcc ggccgcgcct ggcaaatggg tgccgatata ccgtttcgca
     1141 tcaatcagca cacaggggcg gtgcacgtgc gttcggggat gttgcgcacg caatacgatc
     1201 tgtccatcga cggcgtgtcg gtcgagagcg gacgacccat ggtgtcgctg ctgccgctgc
     1261 cggggtgggc ctggatcttt atcggggcgt gcgccctgat cccgatcatc acgctgggcg
     1321 gcgcggtccc ggcgggcatc ggcgcggggg gcgcgttcgg ttgcgccagc gtggcccgcg
     1381 atcccaaccg ggccgcatcg caccgggtgg gtatctgcac gggcattgcc gccggctgct
     1441 gggggctgct cctgggcttc ctggccctgg tagcgggggc ctgagcggga actgagcatg
     1501 gaagggccgg ttggacggat gccgccaacc ggccccatag tgacccatat gcgcggcccg
     1561 cgcggctaat cgacctgcaa gacgacggcg tcgccctggg cggtgcccga gcagatggcg
     1621 gccaggccga ggccgccgcc gcgccggtgc aactcataga tgagcgtcgt cagcaggcgg
     1681 gcgccgctgg cgccgatggg gtggccgagc gcgatggctc cgccgttcac gttcacgatc
     1741 tccggatcca cgccgagccg ccgcgcgctg atgatcgcca cgctggcgaa cgcctcgttg
     1801 atctcgacca ggtcgatgtc ctggatggtc aggcccagcc gcttgagcgc gttctgggcg
     1861 gccatggccg gggtgtaggc caggtagggc gtgtcccagg cggcctcgcc gtagcccagg
     1921 atgcgggcca gcggcttgag gccgtgcgcc ttggcatagg cggcggaggc aacgaccagt
     1981 gcgcccgcgc cgtcgttgag gccgggagcg ttgcccgcgg tgatggtgcc ggtggggctg
     2041 aacaccggct tgagcttggc caggccctcc agggtggtgt cgcggcgcac cgcttcgtcc
     2101 tggtccacgg ccaccggggt gcctttcttc tgcggcaccg gcaccggcac gatttcctgc
     2161 gcgaagatgc cgttgtcctg ggcggcggcg gcgcgcatct ggctgcggta ggcccaggcg
     2221 tcctgttcct cgcggcccac cttctcctcg gcggccaccg tggagccgtg ctggcccatg
     2281 tggacgttca gcagcgggtc ggtcaggccg tcgtagacca cgccgtcgat cagctcgccg
     2341 ttgcccatgc gctggcccca gcgcgcgccc ctgaggtagt agggcgcgtt gctcatgctc
     2401 tccatgcccc cggccacgat cacctggtgg cggtcgaggc ggatcaggtc gccggccagc
     2461 gcgaccgcgc gcatgccgct ggcgcagacg cggttgatcg tatcggacgt gacctcgcgg
     2521 cccagcccag ccttgaaggc cgcctgccgc gacggcacct ggcccagccc ggcctgcacc
     2581 acgcagccca tgatgttgtg cgtgacatct tccttgggga tatcggcccg gcggatggcc
     2641 tcgccgatga cacaggcgcc caggtccacc gcgcccacac tcatcaaact gccgccgaac
     2701 ttgccgaagg gcgtgcgtac cgcccccaga atgaccggat gatcgtcgtt cgccatagtg
     2761 ttgctcgctt tctccctctc cattgaaggt tgctttccag cgtcgccccc ggtgggcgta
     2821 cgctctagtg taccatcagc ccgcgaatgg cgcaaccggc gcgcggcttg acagtgctgg
     2881 agcggctctg ctatagttgt ggcgtttcca gggcctcttt tt
//
LOCUS       DASUYC010000721         2279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1033471, whole genome shotgun sequence.
ACCESSION   DASUYC010000721 DASUYC010000000
VERSION     DASUYC010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033471"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..668
                     /locus_tag="VF276_11780"
     CDS             228..668
                     /locus_tag="VF276_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607583.1"
                     /translation="MRIATPGPARSHLLLLATLVLVLGGGSVWLALGTARPLEAPPLP
                     SANTALPSATPAPASARSPTATLTAAVAPTATATPPSQAGRAIPSRNFVAAGHNNRPA
                     NMVGYLQKHNQGSTETGFGHPTWLCLINTHPATAHGEAPHVHAI"
     gene            652..1056
                     /locus_tag="VF276_11785"
     CDS             652..1056
                     /locus_tag="VF276_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002628389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX6607584.1"
                     /translation="MSTPSDNSAIARRYLQAIEGGRTGADLAAFFTPDVVQEEFPNRL
                     VAQGARRDLAALLAGAEQGQRVVSRQQYEVRNVLATGDQVALEVVWTATLNVPVGNLP
                     AGGEMRAHFAVFLEFRDGQIAAQRNYDCFDPF"
     gene            1110..>2279
                     /locus_tag="VF276_11790"
     CDS             1110..>2279
                     /locus_tag="VF276_11790"
                     /inference="COORDINATES: protein motif:HMM:NF023855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme N-terminal
                     domain-containing protein"
                     /protein_id="HEX6607585.1"
                     /translation="MITLGRNITLNLEAALSHEWLVTNGLGGYAAGTIPGVNTRRYHG
                     LLIAALKPPVERTMLVAKCDEEVEMDGVTYFLGANEYPDDRIHPGGFVHLESFEYRNG
                     MPSFLYRVGPAFLRKTIWMEYGRNTTYLRYSVVDSPYPMNLIVRPFVNYRDHHSLTKG
                     RLDWNFEVLVRPIGGGCSIKATPEAPPLYIIALPRAAFTPSGVWYWNFLYRAERERGM
                     DYLEDLYLPGVFRSQLTTGQSVTMIATAEPPPQVDRAVDAALTRMETREARLLRQAGV
                     ALDAADRDAADPAADDGQAFIAQLVQAADQFLVYRGEASQPDQAERAAARTPAPVVYM
                     RAPNAPQRGGRPSTILATAAQPPPPTLNPAEQEDMRPRTVIAGYPWFTDWGRDTMI"
BASE COUNT          380 a          840 c          710 g          349 t
ORIGIN      
        1 ccctgaaggg gcgggctagc cttacgaagc ccgcctgcgc gggctggacg agcttaggcc
       61 gccttcgcgg ccttcgcagc cgtagcgggc ggcttgtggg gtatacccca gcgcggctac
      121 gccgtcccca cgcttgacac ccctcacggg cgttgctaga atagccgcag agagaagggg
      181 catgctatgc cggctatccg ccgcataacc caatactggg gccggctatg cggatagcaa
      241 cgcccggccc cgcccgctcc cacctgctgc tcctggcgac cctcgtgctg gtgctgggcg
      301 gcggcagcgt ctggctggcc ctgggcaccg cccggcccct ggaagcaccg cccctgccct
      361 ccgccaacac cgctctgccc tccgccaccc cggcgccggc gtccgcacgc agccccaccg
      421 ccaccctcac cgccgccgtc gcgcccaccg ccaccgccac gcccccgtcg caggccgggc
      481 gcgcgatccc aagccgcaat ttcgtcgccg ccgggcataa caaccggccc gccaacatgg
      541 ttggctatct acagaagcat aatcaaggca gcacggaaac agggttcggc catcccacct
      601 ggttgtgctt gatcaacacg cacccggcta cggcccatgg agaggcccca catgtccacg
      661 ccatctgaca atagcgcaat cgcccggcgc tacttgcaag ccatcgaggg cgggcgcacc
      721 ggcgcggatc tcgcagcgtt tttcaccccg gatgtcgtgc aggaagaatt cccgaaccgc
      781 ctggtggccc agggggcgcg ccgcgacctg gccgccttgc ttgcgggcgc ggagcaaggc
      841 cagcgggtgg tttcccgcca gcagtatgag gtgcgcaacg tgctggcgac cggtgatcag
      901 gtcgcactgg aggtcgtgtg gacggccacg cttaacgtgc ccgtcggtaa tttgcctgcg
      961 ggcggtgaga tgcgcgccca tttcgcggtg ttcctggaat ttcgcgacgg ccagatcgcc
     1021 gcccagcgca actacgactg ctttgacccc ttttgacatc ccgccacggg taggcgacgg
     1081 cgtagccgat cagaatcagg agcaaaccta tgattacgtt gggtcgtaat atcacgctaa
     1141 acctggaggc ggcgctaagc catgagtggc tggtgacgaa cgggctgggc ggctatgccg
     1201 cgggcacgat ccccggcgtc aacacccgcc gctaccacgg cctgctgatc gccgccctca
     1261 agccgccggt cgagcgcacc atgctcgtgg ccaagtgcga cgaagaagtc gaaatggacg
     1321 gcgtcaccta tttcctgggc gcgaacgaat acccagacga ccgcatccac cccggcggct
     1381 tcgtccacct ggagtcgttt gaataccgca acggcatgcc cagcttccta taccgggtcg
     1441 gccccgcgtt tctgcgtaaa accatctgga tggagtacgg gcgcaacacc acgtatctgc
     1501 gctacagcgt cgtggacagc ccctacccca tgaacctgat cgtgcgcccg ttcgtcaatt
     1561 accgcgacca ccatagcctg accaagggcc ggctcgactg gaacttcgag gtgctggtgc
     1621 ggcccatcgg cggcgggtgc tcgatcaagg ccacgcccga ggccccgccg ctgtatatca
     1681 tcgctttgcc gcgcgccgcg ttcacgccgt ccggcgtctg gtactggaac ttcctctacc
     1741 gcgccgaacg cgaacgcggc atggattacc tggaagacct ctatctgccg ggcgtcttcc
     1801 gcagccagct cacgacgggc caatcggtga cgatgatcgc gaccgcggag ccgccaccgc
     1861 aggtggatcg cgcagtcgat gccgcgctga cccgcatgga gacgcgcgag gcccggctgt
     1921 tgcgccaggc gggcgtggcg cttgacgcag cggatcgcga tgccgccgac cccgccgccg
     1981 acgacgggca ggccttcatc gcccagcttg tgcaggccgc cgaccagttc ctcgtttatc
     2041 gcggcgaagc gtcccagccg gaccaggccg agcgcgccgc ggcccgcacc ccggctccgg
     2101 tcgtctatat gcgcgcgccg aacgcgccgc aacggggcgg gcgccccagc acgatcctgg
     2161 cgaccgccgc gcaaccgccg ccgcccacgc tcaatcccgc cgagcaggag gatatgcgcc
     2221 cgcgcacggt catcgccggc tacccctggt tcacggattg gggccgcgac accatgatc
//
LOCUS       DASUYC010000722         3634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1840275, whole genome shotgun sequence.
ACCESSION   DASUYC010000722 DASUYC010000000
VERSION     DASUYC010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1840275"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1176)
                     /locus_tag="VF276_11795"
     CDS             complement(<1..1176)
                     /locus_tag="VF276_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX6607586.1"
                     /translation="MRLHVHLSSDWVLDTEMREYPPGEEVDFCIIGTGAGGGVLAQRL
                     ARYGFSVVALDAGPWHDTETDMVSDEAGSGPLYWNDLRITGGTEPLELGANNSGKGVG
                     GSTIHYAGFCPRFHPSDFRVRTQAGVAADWPIAYEDIEPYYEQMEREYPVSGPAYYPW
                     GKPHGYPYAPLEAGTAGQQLIRGCTRLGIPVVAGGPVAIPAGRVGKRPHCIMRGFCLL
                     GCKVGAKSSTMVSHIPDAIAHGAEIRTRCMAYEVPVDGEGRVTGVRYYRTDDNGKVHD
                     EQQRARAVVVAGYSIETPRLLLNSTSGRFPAGLANSSGKVGKYLMAQAGPVVWGRFDE
                     PIRQYKAPPACACTEEFYETDPHNDFVRGYALQTVSPLPIAMAHVLTEDGMFGADLLA
                     "
     gene            complement(1173..1796)
                     /locus_tag="VF276_11800"
     CDS             complement(1173..1796)
                     /locus_tag="VF276_11800"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEX6607587.1"
                     /translation="MSSERYPGADSLAQRGHWDEATRRVIMDRVHNVPPFRYFAGHWR
                     ATLEALCARVIPQDDRRPEDRVPLAPWIDARCYQHTISGFRFEDMPPDEVAWEWGLQG
                     LDQVAQALHQADFVDLDGARQDKVLEAIRGGNPPGEVWSRMPVRRWWVYIALRQITGV
                     YYAHPRAWDEIGFGGPAYPRGYFALNNGAPEPWEAREAPDGTRRNGR"
     gene            complement(1799..2770)
                     /locus_tag="VF276_11805"
     CDS             complement(1799..2770)
                     /locus_tag="VF276_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607588.1"
                     /translation="MSLLHRNRHLVFDAGREIALAEREIREGRFQRSMALIAAFSAIV
                     SGFEAYVQHQRGAFSHWLMWTPVWLTPPTALVSAVALVSERVARRVLPVIALVSLADG
                     VIGFWYHLRGIGKLPGGWKLGQYNIVMGPPIFAPLLTCMVGLLAGLAGLLRRERVALP
                     APEVVRARDAIRRLVQREDPPSALDQFTAAVAHGRFQRRLALTSAFFAILAGGEAYFE
                     HLRGSFNQRVMWTPVWVTPFMVLAAVGAAASERMARRVLPVVSLVTLLDGMLGFLLHL
                     RGIRQMPGGFANLRFNITMGPPLFAPLLFSSVGLLGFVAALLRRKEH"
     gene            complement(3084..3293)
                     /locus_tag="VF276_11810"
     CDS             complement(3084..3293)
                     /locus_tag="VF276_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607589.1"
                     /translation="MGDKWTDPAAWLEAGLALFGALLLGGLALLLGGRLSGGVVRGAR
                     HRAGPPAAGSVPETRHQNIGTSSPR"
BASE COUNT          572 a         1354 c         1167 g          541 t
ORIGIN      
        1 cgccagcagg tccgcgccga acataccgtc ctcggtcagc acatgggcca tggcgatggg
       61 cagcgggctg accgtttgca gcgcgtagcc gcgcacaaag tcgttgtgcg ggtcggtttc
      121 gtagaactcc tcggtgcagg cgcaggcggg cggcgccttg tactggcgga tcggctcgtc
      181 gaagcggccc cagaccaccg gcccggcctg cgccatcagg tacttgccga ccttgccgct
      241 cgaattcgcc agcccggcgg ggaaccgccc cgaggtcgag ttgagcagca gccgcggcgt
      301 ctcgatggag tacccggcca cgaccacggc ccgcgcccgc tgctgctcgt catgcacctt
      361 accgttatcg tcggtgcggt agtagcgcac gcccgtcacc cggccctcgc cgtccaccgg
      421 cacctcgtag gccatgcagc gcgtgcggat ctccgcgccg tgggcgatgg cgtcggggat
      481 gtggctgacc atggtgctgc tcttggcgcc cactttgcag cccagcaggc agaagccgcg
      541 catgatgcag tgcgggcgct tgcccacgcg ccccgccggg atcgccaccg gcccgcctgc
      601 caccaccggg atgcccagcc gggtgcagcc gcggatcaac tgctggcccg ccgtgcccgc
      661 ctccagtggc gcgtaggggt agccgtgcgg tttgccccag ggataatagg ccgggccgga
      721 caccgggtac tcgcgctcca tctgctcgta gtacggctcg atgtcttcgt aggcgatggg
      781 ccagtcggcg gccacgccgg cctgcgtgcg cacgcggaag tccgacgggt ggaagcgcgg
      841 gcagaacccc gcgtagtgga tggtgctacc gcccacgccc ttgcccgaat tattcgcgcc
      901 cagctccagg ggctcggtgc cgccggtgat gcgcaggtcg ttccagtaga gcggccccga
      961 cccggcttca tccgacacca tgtcggtctc ggtgtcgtgc cacggcccgg cgtcgagcgc
     1021 caccaccgag aagccgtagc gcgccagccg ctgggccagc acgccgcccc cggcgcccgt
     1081 gccgatgatg caaaagtcca cttcctcgcc gggcgggtat tcccgcatct cggtgtccag
     1141 cacccagtcc gacgacagat gtacgtgtaa tctcaccgcc cattcctccg cgtgccgtcc
     1201 ggggcctcgc gggcctccca gggttccggc gcgccgttgt tcaacgcaaa atagccgcgc
     1261 gggtaggccg gaccgccgaa gccgatctcg tcccaggcgc gcgggtgcgc gtaatacacg
     1321 ccggtgatct ggcgcagcgc gatatagacc caccagcggc gcaccggcat gcggctccag
     1381 acctcgcccg gcgggttgcc gccgcggatg gcttcgagca ctttgtcctg ccgcgcgccg
     1441 tccaggtcca caaaatcggc ctggtgcagc gcctgggcca cctggtccag cccctgcaag
     1501 ccccactccc aggccacctc gtcgggcggc atgtcctcga agcggaagcc ggagatagtg
     1561 tgctggtagc agcgggcgtc gatccagggc gccagcggca cgcggtcctc gggccgccgg
     1621 tcgtcctgcg ggatgacccg cgcgcagagc gcctccagcg tggcgcgcca gtggcccgcg
     1681 aagtagcgaa acggcggcac gttgtgcacc cggtccatga tcacgcggcg cgtggcctcg
     1741 tcccagtggc cgcgctgggc caggctgtcg gcgccggggt agcgttcgct gctcataatc
     1801 aatgctcctt ccggcgcaac agcgcggcga cgaagccgag caggcccacc gacgaaaaca
     1861 gcagcggcgc gaacagcggt ggtcccatgg taatgttgaa gcgcaggttg gcgaagccgc
     1921 ccggcatctg gcggatgccg cgcaggtgca gcaggaagcc cagcatgccg tcgagcaacg
     1981 tgaccagcga caccaccggc aacacccgcc gggccatgcg ctcgctggcc gccgcaccca
     2041 ccgccgccag caccatgaac ggtgtcaccc agaccggcgt ccacatgacg cgctggttga
     2101 agctgccgcg caggtgctcg aagtaggctt cgcccccggc caggatggcg aagaacgccg
     2161 aggtcagggc caggcgccgc tggaaccggc catgggccac cgccgcggtg aactggtcga
     2221 gcgccgaggg cgggtcttcc cgctgcacca ggcggcggat ggcgtcgcgc gcccgcacca
     2281 cctcgggcgc gggcagggcc acccgttcgc ggcgcagcag cccggccagc ccggccagca
     2341 ggccgaccat gcaggtcagc agcggcgcga agatgggtgg ccccatgacg atgttgtact
     2401 ggcccagctt ccacccgccc ggcaacttgc cgatgccgcg caggtgatac cagaagccga
     2461 tcacgccgtc ggccagcgac accagggcga tcaccggcag cacccgccgc gcgacccgct
     2521 cgctcaccag cgcgaccgcc gacacgaggg ccgtgggcgg cgtcagccac acgggcgtcc
     2581 acatcagcca gtggctgaac gccccgcgct gatgctgcac atacgcctcg aatccgctga
     2641 cgatggccga gaacgcggcg atcagcgcca tgctgcgctg gaagcgcccc tcgcggatct
     2701 cccgttcggc cagcgcgatc tcccgcccgg cgtcgaacac cagatgccgg ttccgatgca
     2761 acaaactcat acaactccct ctctcagcag ccctggtccc tgtctgtgtc actctgagcg
     2821 cagcgaagag tctcgtctcc cccgccgcct gtcactctaa gcgcagcaaa gagtctcgtc
     2881 tcgcttctgc ctaagacgga gattcttcgc tccgctcaga atgacaatcc agccgctgtg
     2941 ctgtccgtgt cgtgtcactc tgcgtggagc gaagagtctc gtccccgccc tctgtcactc
     3001 tgagcgcagc gaagagtctc gtcgcgcttg cttacaagac ggagattctt cgctccgctc
     3061 agaatgacaa gttggcagcg tactcaacgt ggtgacgacg tgccgatatt ctggtgccgg
     3121 gtctccggca cactgcccgc tgcgggcggt ccggcgcgat gccgggcgcc gcgcacgacc
     3181 cccccgctca ggcgcccgcc cagcagcagc gccagcccgc cgagcaacag cgcaccgaac
     3241 agggccaacc ccgcctccag ccacgccgcc ggatcggtcc acttgtctcc catcacaatc
     3301 tcctttgccg cggtaggagc ggacacgccg gcccacccct accgcgccca gcggcctgcg
     3361 gcgccaatcg tcgtactgac cactgaccca ctgaccactc gtcgtattga ccaccgacca
     3421 ctcgtcgtac tgaccactga ccactgacca ctgaccactc gcctagacca cctcgcgcac
     3481 cttgtccttg agcacggtct tgatgatgtc catgcgatgg cgctcgccgc gggccagggc
     3541 ctcggcgaag tggatcgcct gctcggtcgt gatcttgccc ggcatcggcg gttcgttcgg
     3601 gtccaccacc gcctcgatca ccaccgggcc ggtc
//
LOCUS       DASUYC010000723         2329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1285287, whole genome shotgun sequence.
ACCESSION   DASUYC010000723 DASUYC010000000
VERSION     DASUYC010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285287"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="VF276_11815"
     CDS             complement(<1..1122)
                     /locus_tag="VF276_11815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX6607590.1"
                     /translation="MFAPARWRAARRVTGLRRVLVLKPCCFGDVVMATAALAALQRAY
                     PAAAITLAVGAWSRPAVAANPRLRALIDSDPVGTAPIRRTWRAYLELARRLRRERFDA
                     VLVLDRSPLLGLLAWLAGAPVRAGLDSGGRGFALTHRVPCPPAVARNEVEWYLDVVRA
                     LGVPVDPATRTEFYPTPADTAEADAALAALPLDPVRDWLIALHTGGGSNPGMRMPAKR
                     WAPARWAAVLARLLTTYPQAQVLLLGAAGPDDQAAAAQIRAGVPAEQAGRMHDLTGCF
                     GWGALGALAARCRLFLGPDTGAMHLAVAAGAPVVAVFGPSDPRRYAPWDPGGRSRAVG
                     GTAGGVDLAARAAQLAGVAFNEIAPVDAVWAAVQDVLGPP"
     gene            complement(1302..1778)
                     /gene="fabZ"
                     /locus_tag="VF276_11820"
     CDS             complement(1302..1778)
                     /gene="fabZ"
                     /locus_tag="VF276_11820"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEX6607591.1"
                     /translation="MAEETTVLSNVEIQQILPHRFPFLLVDKMVALEPGKSGVGIKSV
                     TFNEWYFPGHVPGYPVMPGVLMVEALAQVAGIVALSLPEHHGKMGFFAGIDKIRFKRQ
                     VKPGDTLRLEVTLTRMRPPVCQATVQATVDGEVACAGDIMFMLVETGGLGSPSSAE"
     gene            complement(1823..>2329)
                     /locus_tag="VF276_11825"
     CDS             complement(1823..>2329)
                     /locus_tag="VF276_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607592.1"
                     /translation="QLQDQAQVMRLNEFQNAATIIARYPVFGVGFGQAGELDLTTGVS
                     SVYLTVAERTGLVGLTAFVVVMIAFFALLAPWLRARPLPPTDAAAILDAAALGAGAAV
                     LGALVVGVADHYFFNIEQTHLAALLWFCLALGMAARRLLMDEVRAARPQATEATEAEP
                     VASAAPSL"
BASE COUNT          375 a          878 c          790 g          286 t
ORIGIN      
        1 cggcggcccc aggacatcct gcaccgccgc ccagacggca tccaccgggg cgatctcgtt
       61 gaaggccacg ccggcgagtt gcgcggcgcg cgccgccagg tccacgccgc ccgccgtgcc
      121 gcccacggcc cggctgcgcc cgccggggtc ccagggcgcg tagcggcgcg ggtcggacgg
      181 cccgaacacg gccaccaccg gcgcgcccgc cgccacggcc aggtgcatgg ccccggtgtc
      241 cggccccagg aacaggcggc agcgggccgc cagcgccccc agcgcccccc agccgaagca
      301 tccggtcagg tcgtgcatac gacccgcctg ctctgccggc acacccgccc ggatctgcgc
      361 ggcggcggcc tggtcgtcgg ggccggcggc gcccagcagc agcacctggg cctggggata
      421 ggtggtgagg aggcgggcca gcacggcggc ccagcgggcg ggcgcccagc gcttggccgg
      481 catacgcatg ccggggttgc tgccgccgcc cgtgtgcagg gcaatcagcc aatcgcggac
      541 gggatcgagg ggcagggcgg ccagggcggc atcggcctcg gcggtgtcgg cgggggtggg
      601 gtaaaactcg gtgcgcgtgg ccggatcgac cggcacgccc agcgcccgca ccacgtccag
      661 ataccactcg acctcgttgc gggccaccgc cggcgggcac ggcacccggt gcgtcagcgc
      721 gaagccccgg cccccactgt ccagcccggc acgcaccggc gcgcccgcca gccaggccag
      781 caaccctagc agcggcgacc ggtccagcac cagcacggcg tcgaaccgtt cgcgccgcag
      841 ccgccgggcc agctccaggt aggcacgcca ggtgcggcga atgggcgccg tgcccaccgg
      901 atcgctgtcg atcagcgcgc gcaggcgcgg gttcgccgcc accgccgggc gtgaccacgc
      961 ccccaccgcc agcgtaatcg ccgccgcggg ataggcccgc tggagggcgg ccagcgccgc
     1021 cgtcgccatc accacatcgc cgaagcagca cggcttcagc accagcaccc gccgcagccc
     1081 cgtcacgcgc cgcgccgccc gccaccgcgc cggcgcaaac acccacgtca gccctctcca
     1141 cagtgctgag tgctgatcgc gcagcggtgc cgcaggcaca ttgctgagtg ctgagtacgg
     1201 agtacgacga gtgcgagaag ctacgagaga aggcacggga ttacccggtg tccttccctc
     1261 gtcgctactg accactgacc actgaccact gaccacccgt ctcactcagc gctcgacgga
     1321 ctacccaatc cgccggtttc gaccagcatg aacatgatgt cgccggcgca ggccacctcg
     1381 ccgtccaccg tggcctggac ggtcgcctgg cagacgggcg ggcgcatgcg ggtcagtgtc
     1441 acctccaggc gcagcgtgtc gcccggcttc acctggcgct tgaagcggat cttgtcgatg
     1501 ccggcgaaaa agcccatctt gccgtgatgt tcgggcaggc tcagggccac gatgcccgcc
     1561 acctgggcca gcgcctcgac catcaacacg ccgggcatga ccgggtagcc gggcacgtgc
     1621 ccggggaaat accactcgtt gaaggtcacg ctcttgatgc cgaccccgct cttgcccggc
     1681 tccagggcca ccatcttatc gaccagcaaa aacgggaagc ggtgcgggag gatctgctgg
     1741 atctccacat tgctgagcac cgtcgtttct tccgccatgg gacactcctt cctggtatac
     1801 gactactaat tgaaatgggc gatcataggc tcggcgcggc agaggcgacc ggttcggcct
     1861 ctgtggcctc cgtcgcctgc ggccgggcgg cgcgcacttc gtccatcaac aggcggcgcg
     1921 cggccatgcc cagcgcgaga cagaaccaga gcaacgcggc caggtgcgtc tgctcgatat
     1981 tgaagaagta atggtcggcc acgccgacga cgagcgcgcc caataccgcc gcgcccgcgc
     2041 ccagcgccgc cgcgtcgaga atggccgccg cgtcagtagg cggcagcggg cgggcgcgca
     2101 gccacggcgc cagcagcgcg aaaaaggcga tcatcaccac gacgaacgcg gttaatccga
     2161 ccaggcccgt ccgctcggct acggtcagat agacgctgga cacgccggtg gtcaggtcca
     2221 gttcgcccgc ctggccgaag ccgacgccga acaccgggta gcgggcgatg atcgtcgccg
     2281 cgttctggaa ctcgttgagg cgcatgacct gggcctggtc ctgcaactg
//
LOCUS       DASUYC010000724         3876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_794263,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000724 DASUYC010000000
VERSION     DASUYC010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794263"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            453..1604
                     /locus_tag="VF276_11830"
     CDS             453..1604
                     /locus_tag="VF276_11830"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX6607593.1"
                     /translation="METTTSDAPAGSPQAPYAARLERAREQLRHAGLDYCFIGPSSDL
                     RYLTGLNLHLSERLSLLIVPANGPSHLVIPAFEAPGLPPLPESIRVAAWEENEDPARL
                     VARLLQPQGESQGVTGTIAVGDQLWAVFLLRLQAALPRATFSTAALVLEPLRLVKDAA
                     EVAAMAEAGAQADAVFAEIQQMRFSGQSEYQIAEAIAGRLRARGLRIEWGPIVGSGPN
                     GASPHHTASDRIIQPGELVVLDFGGVLNGYNADMTRTVAVGHEPDAEMRQVYELVRQG
                     QEKGVQAAQPGLTGEQLDSVVRDFFTQAGYGQYFTHRLGHGIGLDAHEPPYLVQGGQM
                     VLAPGMAFTVEPGLYLKGRFGVRIEDTVVLTPTGARRMNNAPRELIIVQ"
     gene            complement(1632..1880)
                     /locus_tag="VF276_11835"
     CDS             complement(1632..1880)
                     /locus_tag="VF276_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HEX6607594.1"
                     /translation="MQREPFNSSTILTAGYDPASETLEIEFRNHRLYRYLDVPTEVWQ
                     GLRAAQSAGAYFTANIRNDYECWRQVNLAGIKHRNATQ"
     gene            2209..3171
                     /gene="ispH"
                     /locus_tag="VF276_11840"
     CDS             2209..3171
                     /gene="ispH"
                     /locus_tag="VF276_11840"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX6607595.1"
                     /translation="MEIKLAREMGMCFGVKMTLDHIAEAGQQHANIDTLGTLVHNPQL
                     VERLAEQGVQVKDGLDQVQAPTVAITAHGAAPDIAARAQAAGRTVIDTTCPLVTRVQQ
                     TAKKEADEGSWVIVYGDSFHPETKGILGWANSTLDGTVPPTPGSLVERKRPKNLRRAI
                     CARSVADIDAALEAAGTPGAKVMRIALLSQTTMNVAWFKQFVTEVVGRFLEFGTEIHV
                     HNTICLPTAKRQPAAEALAQEVDLVIAIGGYESANTRHLAELAAKYCPSHHIERPEEL
                     DPAWFEGVQKVGVTAGASTPDWVINDVLDAIKAIDARQQAAVPA"
     gene            complement(3362..>3876)
                     /locus_tag="VF276_11845"
     CDS             complement(3362..>3876)
                     /locus_tag="VF276_11845"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6607596.1"
                     /translation="ENWMQRPLRDPVPAPALPLPTVTWTAETAPQFYAAYYTSFATRR
                     RADAPAPAAEDWIADYADDPDFRPDLSLLALDGDRPVGFIAAGVLHLPHSDQPVGWFS
                     QVGVDPAWRGRGLGAGLMGAVLAGFQREGFGAVGLHVNIDNPNAIALYERLGFELVGR
                     RAKYSKPRTP"
BASE COUNT          670 a         1306 c         1288 g          612 t
ORIGIN      
        1 gcgcggacaa ccatgatctc aggtctgtaa caagcgggaa cggtattgat ctagcgacct
       61 gcggtactga acgagtcgca gatcagcctg gccggtgagg aaagccctgg gaatggcccg
      121 tgagtaaatg ttcttatccg ctccgcactt gacagttatc tggctccgtg ctaggatagg
      181 ctaggcccgc cacgatgcgc ggccaccgcc tcccggcggc tcctcattcc atgttccaac
      241 tcctagggag agaaagggga attacggcca tgggtgataa aagtccgaag gataaagaga
      301 agaagaagcc aaaagccgac aagaaagctg ctccggcgtc gtccccggcg aagaagtagt
      361 ttgtcgcagc cacgccgggc tgtagtcgtg ccgccttccg tgggccggcg cgactgtagc
      421 ccggcatttt ctttgggagg acccgatccc tgatggagac gaccacctct gacgcgcccg
      481 ccggcagccc gcaggcgccg tatgccgccc gcctggaacg ggcgcgcgag cagttgcgcc
      541 atgccggcct cgattattgt tttatcggcc cctcgtccga tctgcgctat ctgaccgggc
      601 tcaatctcca cctcagcgag cgcctctcgc tgctcatcgt ccccgcgaac gggccgagcc
      661 acctggtgat ccccgccttc gaggcgcccg gcctgccgcc gctcccggaa agcatccggg
      721 tcgcggcgtg ggaggaaaac gaggacccgg cccgcctggt ggcccggctg ctgcaacccc
      781 agggcgaaag ccagggcgtc accggcacca tcgccgtggg cgatcagctc tgggcggtct
      841 tcctgctgcg attgcaagcc gcgctgcccc gcgccacctt cagcacggcg gcgctggtgc
      901 tggagccgtt gcggttagtg aaagacgccg ccgaagtggc ggccatggcc gaggccggtg
      961 cccaggccga tgccgtcttc gccgaaatcc agcagatgcg cttcagcggg cagagcgagt
     1021 accagatcgc ggaggccatc gcgggccggt tgcgggcgcg cggcctgcgc atcgagtggg
     1081 gacccatcgt gggcagcggc cccaacggcg cctccccgca ccataccgcc agcgaccgta
     1141 tcatccagcc gggcgaactg gtggtgctcg acttcggcgg ggtgctgaac ggctacaacg
     1201 ccgacatgac gcgcaccgtg gccgtgggcc acgagcccga tgccgagatg cgccaggtgt
     1261 acgaactggt ccgccagggg caggagaagg gggtgcaggc ggcccagccc ggcctcaccg
     1321 gcgaacaact cgactcggtg gtgcgcgact tcttcaccca ggcgggctac ggccagtatt
     1381 tcacgcaccg gctgggccat ggcatcggcc tcgacgccca cgagccacct tacctggtgc
     1441 agggcgggca aatggtgctg gcccccggca tggccttcac cgtggagccc ggcctctatc
     1501 tcaagggccg cttcggggtg cgcatcgagg acaccgtggt gctgaccccc accggcgccc
     1561 ggcgcatgaa caacgccccg cgcgaactga tcatcgtgca atagcgggcc ggcgggaggc
     1621 ttcccgccgg cctattgcgt cgcgttgcgg tgcttgatcc cggcgagatt gacctgccgc
     1681 cagcactcgt aatcgttgcg gatgttcgcc gtgaagtacg cgcccgccga ttgggccgcc
     1741 cgcaagccct gccagacctc ggtcggcacg tccaggtagc gatagagccg gtggttgcgg
     1801 aactcgattt ccagcgtctc gctcgccggg tcgtagcccg ccgtcagaat cgtgctcgaa
     1861 ttgaacggtt cgcgttgcat actgctgcct cctgcgcgtc tgtggcgtgc tcatctatgc
     1921 gccccttcta acgaagttcc ccgccgattg acacgccgcg tgccaagttg gtatagttag
     1981 cgtggtcacg tgcgccttgc gcgcccgtcc tccggcacga tgggaaacta tgccggcaaa
     2041 tgcgccgtta tctaagtgag ttcgcgcgaa tccgatcatg cgatccgatg tgtaagcgcg
     2101 aagggcatag cagcgttagt attcgtggat cacagggatt cacgccaggg atcagactcg
     2161 cggggccgcc ggttgggccg cgcgggccgg cgaggagtag acacactgat ggaaatcaag
     2221 ctcgcacgcg agatgggcat gtgcttcggg gtcaagatga ccctcgacca catcgcggaa
     2281 gcagggcagc agcacgcgaa catcgatacg ctgggcacgc tggtgcacaa cccgcagttg
     2341 gtggaacggc tggccgaaca aggcgtgcag gtgaaggatg ggctggatca ggtccaggcg
     2401 ccgaccgtcg ccatcaccgc ccacggcgcg gcgcccgaca tcgcggcgcg cgcccaggcc
     2461 gccgggcgca cggtcatcga caccacctgc ccgctggtca cgcgcgtgca gcagacggcc
     2521 aagaaggaag ccgacgaggg gtcctgggtc atcgtctatg gcgacagctt ccacccggag
     2581 accaagggca tcctcggctg ggccaactcc acgctcgacg gcacggtgcc gcccacgccg
     2641 ggcagcctgg tcgagcgcaa gcggcccaag aacctgcgcc gcgccatctg cgcccgtagc
     2701 gtcgccgata tcgacgccgc gctggaggcc gccgggacgc ccggcgccaa ggtgatgcgc
     2761 atcgccctgc tgtcgcagac gacgatgaac gtggcctggt tcaagcagtt tgtcaccgaa
     2821 gtggtgggac gcttcctgga gttcggcacc gagatccatg tccacaacac catctgcctg
     2881 cccacggcca agcgccagcc cgccgccgag gcgctggccc aggaggtgga cctggtgatc
     2941 gccatcggcg gctacgagag cgccaacacg cgccacctgg ccgaactggc cgccaagtac
     3001 tgccccagcc accacatcga gcgccccgaa gagctggacc cggcctggtt cgagggtgtg
     3061 cagaaggtcg gcgtcacggc aggcgccagc acccccgact gggtcatcaa cgacgtgctc
     3121 gacgccatca aggccatcga cgcccgccag caggccgccg tcccggcgta gtgctgagtg
     3181 ctgagtgctg agtgaattta gagggcaacg agggccgcgc acttgcgcgg cccttttgat
     3241 atgctgttgc tgcacctaac accgtagcca gcgccctgtt gtgtgtagat gcgcgattta
     3301 tcgcgccgta ctcgttggtc tttagcgtgt actgttcccc aacaggtcgt cggaagttac
     3361 attaaggcgt gcggggcttg gagtacttgg cccgccgccc gaccaactcg aagcccagcc
     3421 gctcgtacag ggcgatggcg ttcgggttgt cgatgttgac gtgcagcccc acggcgccaa
     3481 agccctcgcg ctggaacccg gccagcaccg cgcccattag cccggccccc agcccccgcc
     3541 cgcgccaggc cggatcgacg cccacctgcg agaaccaccc cactggctga tcgctgtgtg
     3601 gcaggtgcag cactccggcg gcgataaacc ccaccggccg gtcgccgtcc agggccagca
     3661 gcgacagatc ggggcgaaag tccggatcgt cggcgtaatc agcgatccag tcctcggcag
     3721 cgggcgcggg cgcatcggct cggcggcgcg tggcgaagct ggtgtagtag gcggcgtaga
     3781 actgcggcgc ggtttccgcc gtccaggtca cggtgggcag cggcagggcc ggcgcgggca
     3841 ccgggtcgcg caacgggcgc tgcatccagt tttcgg
//
LOCUS       DASUYC010000725         3419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_650472,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000725 DASUYC010000000
VERSION     DASUYC010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650472"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VF276_11850"
     CDS             <1..291
                     /locus_tag="VF276_11850"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX6607597.1"
                     /translation="FTARLAAETELRAAKETAEAANHARSAALAQISYELRPAIHAII
                     GDSERLQAAAAGGNGPDPVIRDLQRVAASGKQLLALIDDVHDLARQDGKPQT"
     gene            298..1089
                     /locus_tag="VF276_11855"
     CDS             298..1089
                     /locus_tag="VF276_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013159798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607598.1"
                     /translation="MAQISKEQLLGRLDAIGRSLQDRGTALALLGVGSVGTELDRIDA
                     YSDLDFFVVVRDGYEQAYIAGLDWLEAVYPIAYRFRNTEHGYKLLFADGIYAEFAVFT
                     LAQLAEIDSPPGRIVWKAAGVDEELLQPRSIPGAGGGRSVDELLGEALTNLYVGLGRY
                     HRGERLSAMRFIQGYAVDRILDLAPHIEAAQPAYRDRFGAERRFEQRFPTVARELPHF
                     LQGYDRTPESAAAILAFLARHWPLDPAIRQAITALLPAPLREEPA"
     gene            1482..2840
                     /locus_tag="VF276_11860"
     CDS             1482..2840
                     /locus_tag="VF276_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607599.1"
                     /translation="MSRRGYHSNPYSTEPLRGRKLWRYGDWGNAGEHIGRHWEAVATP
                     ILRDAIGQLRLPIHPGDPAYTPRVLISIVADPDMAAEVQLSGLAHADSLILGFDEEGR
                     VVAEPADFKWSLETAESRQVSATGLARVLNAGLPLLEAAIVAALGELAALAGVTQPDL
                     EVVSGSVGVPARAPEEAATPADEVPEEDVPSGTDLVEDLEERQYAVVTRDGLFFAPRN
                     QANQRFLHGDWNRRKADPLTDDQVWFQDFDPVEFFSPLPGWDIAGMLAGYDRSAGQLS
                     TPDGAERFYRLGAGTRGALIRWHTSIFSTVPADVDVPVELESLRRAQHLWNSTDISLY
                     LDRMMNVRAERENALTEWLREIYGWSRFRSDLGRAGVNPAELESKAGKRRWGFVFGAI
                     QKAVRARVCSDGLLLVDDGQSDMEALENLQKRGAEFHGMATAVARHTITAALAERDPK
                     AT"
     gene            2859..3413
                     /locus_tag="VF276_11865"
     CDS             2859..3413
                     /locus_tag="VF276_11865"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX6607600.1"
                     /translation="MPHHPAHNVPPPPTGEEPTAAPLPVQPGQALLFFGDSITAATPG
                     YLDVLQQVLSHRRPDLDLRLLNAGIPGNTVRDLAARLDRDVLARRPDWVVICIGANDY
                     FGAARGQGGVPLAEFEAVYRALVARLQGAGIDPILMTIPVIGSDEEDSPIPDPRAYNA
                     TIKALAQSAGLRLVDIHRAFYAVY"
BASE COUNT          534 a         1212 c         1156 g          517 t
ORIGIN      
        1 ttcacggcgc ggctggccgc cgagaccgag ttgcgcgccg ccaaggaaac cgccgaggct
       61 gcgaaccacg cccgcagcgc cgccctggcc cagatcagct acgaactccg cccggccatc
      121 cacgccatca tcggcgacag cgaacggctg caagctgcgg cagccggcgg caacgggccg
      181 gatccggtca tccgcgatct acagcgcgtc gcggcctcgg gcaagcaact cctggcgctg
      241 atcgacgacg tacacgacct ggcccggcag gacggcaagc cgcagacgtg aggaccaatg
      301 gcacagattt ctaaagagca gttgctcggg cggctggacg cgattggccg gagcttgcag
      361 gaccggggca cggcgctggc gctgctcggc gtgggctcag tcggcaccga gttggaccgc
      421 atcgacgcct attcggacct cgatttcttc gtcgtggtcc gcgacggcta cgagcaggcg
      481 tacatcgccg gcctcgactg gctggaggcc gtctacccca tcgcctaccg cttccgcaat
      541 accgagcacg gctataagct gctgttcgcc gacggcatct atgccgagtt cgccgtcttc
      601 accctggcgc agttggccga gatcgactcc ccgccggggc ggatcgtctg gaaggcggcg
      661 ggtgtcgacg aggagctact ccagccgcgc agcatccctg gcgccggggg cgggcgctcg
      721 gtcgatgaac tgctcggcga ggcgctgacc aacctctacg tgggcctggg ccgctatcac
      781 cgcggcgaac gcctctcggc catgcgtttc atccagggct acgcggtgga ccgcattctg
      841 gacctggcgc cccacatcga agccgcgcag ccggcctatc gcgaccgctt cggcgcggag
      901 cggcgcttcg agcagcgctt ccccaccgtc gcgcgggaac tgccgcactt tctccagggc
      961 tacgaccgca cgcccgagtc ggcggcggcc attctcgcct tcctcgcccg gcactggccg
     1021 ctagacccgg ccatccggca ggcgattacg gcgttgttgc ccgcccccct gcgcgaggaa
     1081 ccggcctaac ccccctgccc cccttcccgg cgcgggaagg ggcggaatcg cttttgcaac
     1141 tggaagtgac tcccctcccc gcggcgggga ggggcggggg gagaggttgg cccggcgcgc
     1201 cagcgttcta atccgcccgg ttcgccaact gctccccccc gatttgggcc ggtcgaatct
     1261 gtattcttct actgtgtatt cttaacggca acctagcccg cccactacgg gcacttcccg
     1321 ccacggtcga ggtcgccgca cagcacgggg cttgcggact gatagcgttg cggtgtataa
     1381 ctgagcagta gcgtgtttgt ccatagatcg aaagaggatt gctttatgtc aagtgttacc
     1441 ggcggcaccg gcgcggtggc gatgtacagc gaggccggcg gttgagtcgc cgcggttatc
     1501 attccaaccc ctacagcacc gaacccctgc gcgggcgcaa gctctggcgc tacggcgact
     1561 ggggcaacgc gggtgagcat atcggacggc actgggaggc ggtcgccacc cccattttgc
     1621 gcgacgccat cggccaactg cggctcccga tccaccccgg cgaccccgcc tacacgcccc
     1681 gtgtgctgat cagcatcgtc gccgatccgg acatggccgc cgaagtgcaa ctctccgggc
     1741 tggcccatgc cgacagcctg atcctcggtt tcgacgagga gggccgcgtg gtggccgaac
     1801 cggcggactt caagtggtcg ctggaaaccg ccgagagccg gcaggtgtcg gccaccgggt
     1861 tggcccgtgt gctcaacgcc gggctgccgc tgctggaagc ggctatcgtg gccgcgctgg
     1921 gcgaactggc ggccctggcc ggcgtgaccc aacccgacct cgaagtggtc agcggcagtg
     1981 tgggcgtgcc ggcgcgcgcc cccgaggagg cagccacgcc cgccgacgag gtgccggaag
     2041 aggatgtgcc cagcggtacc gacctggtcg aggatctgga ggagcgccag tacgccgtgg
     2101 tcacacgcga cggcctgttc ttcgccccgc gcaaccaggc caaccagcgc ttcctgcacg
     2161 gcgactggaa ccgccgcaag gccgacccgt tgaccgacga tcaggtctgg ttccaggatt
     2221 tcgacccggt ggagttcttc agccccctgc ccggctggga tatcgccggg atgctggcgg
     2281 gctacgaccg cagcgcgggc caactcagca cgcccgacgg tgccgagcgc ttttaccggc
     2341 tgggcgcggg cacgcgcggc gcgctaatcc gctggcatac ctcgatcttc agcaccgtcc
     2401 cggcggacgt ggacgtgccg gtggagctgg agtcgttgcg ccgcgcccag cacctgtgga
     2461 acagcaccga tatttcgctc tacctggacc gcatgatgaa cgtgcgcgcc gagcgggaaa
     2521 acgcgctcac cgagtggctg cgcgagatct acggctggag ccgcttccgc agcgacctgg
     2581 ggcgcgcggg cgtcaatccc gccgaactgg agtcgaaggc gggcaagcgc cgctggggct
     2641 tcgtcttcgg tgctatccag aaggcggtgc gggcgcgggt gtgcagcgat gggctgctgc
     2701 tggtggacga cgggcagagc gacatggaag cgctggagaa cctccagaag cgcggcgccg
     2761 agttccacgg catggctacc gccgtcgccc ggcatacgat cacggcggcg ctggccgagc
     2821 gcgacccgaa ggccacctga cgccgcgata aggaccctat gccccaccat cccgcccata
     2881 acgtcccgcc cccgcccacc ggcgaggaac cgaccgcagc gccgctgccg gtgcagccgg
     2941 ggcaagcgct gctcttcttc ggcgacagca tcacggcggc cacgccgggc tatctcgacg
     3001 tgctgcaaca ggtgctgagc caccgccgcc ccgacctgga tctgcggctg ctcaacgccg
     3061 gcataccggg caacacggtt cgcgacctgg cggcgcggct cgaccgcgat gtgctggccc
     3121 ggcgccccga ctgggtggtg atctgcatcg gcgccaacga ctatttcggc gcggcgcgcg
     3181 ggcagggcgg cgtgccgctg gccgagttcg aggccgtcta ccgcgccctg gtcgcccggt
     3241 tacagggcgc cgggatcgac cccatcctga tgaccatccc ggtcatcggc agcgacgagg
     3301 aagactcgcc catccccgac ccgcgggcgt acaacgcgac catcaaggcg ctggcgcaga
     3361 gcgccgggtt gcgcctggtg gatatccatc gcgccttcta cgcggtctac tagcgcgcg
//
LOCUS       DASUYC010000726         2309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1003197, whole genome shotgun sequence.
ACCESSION   DASUYC010000726 DASUYC010000000
VERSION     DASUYC010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1003197"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VF276_11870"
     CDS             <1..208
                     /locus_tag="VF276_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018996644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607601.1"
                     /translation="RRAVEALGPPAGLDPAAAAAAALDRLAALPGYGGVIVLDTHGRP
                     GFAFNTPRMAHAAMTPDGALFLDI"
     gene            432..1382
                     /locus_tag="VF276_11875"
     CDS             432..1382
                     /locus_tag="VF276_11875"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6607602.1"
                     /translation="MQPKILVPLDGSPLAETVLPRAAAWARATGGVVVLTRVSAPAMV
                     APPTAWTIPTPLVGYENTLHDMDLARDYLTDAAMRLKQTGVEVETTGLDGDPAAAIIA
                     YAEEHPEVTLIAMATHGRSGLSRWMLGSVAEKVLHAAPVPLLMVRPTGNDAPATPASD
                     LQTILVPLDGSIFAEQALEVAFASARATGAALRLISALPVPDDMAATDSGLTPAPVPA
                     QYQTEVAHLTAYLEQQAEPLRRAGISVETQIVNGRPADAILHAATEAHAGLIVMATHG
                     RSGLQRLWLGSVAMKVVQGSTLPVLLVRAREAAVPAAIPA"
     gene            1495..2076
                     /locus_tag="VF276_11880"
     CDS             1495..2076
                     /locus_tag="VF276_11880"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6607603.1"
                     /translation="MQTEILVPLDGSLVAETALPPALALARALGGSLTLLRVTPPTLG
                     AEPLAEAIQAPVITYRPRPQHRDEADAYLAGVTQRFASSGVPMRILVLRGDPATAILQ
                     YAQEAPGVRLIAMATHGRSGVSRWVFGSVAEKVLRATPAPLLLVRPDDDGMAPPPMHA
                     LPYRTIIVPLDGSPFAEQALDQAYAVAAATGAS"
     gene            2089..>2309
                     /locus_tag="VF276_11885"
     CDS             2089..>2309
                     /locus_tag="VF276_11885"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6607604.1"
                     /translation="MAPEPDEFLAAEVGDIPVWRLEDRLAETERLDAYLQQQAARLIN
                     AGLTVEIQVRAGSPAQAILDTAEEAAADL"
BASE COUNT          329 a          806 c          840 g          334 t
ORIGIN      
        1 gcgccgggcc gtggaagccc tgggcccgcc cgccgggctc gatccggccg cggccgcggc
       61 ggccgccctg gatcggctgg cggcgctgcc cggctacggc ggcgtcatcg tgctcgatac
      121 ccacggtcgt cccggcttcg ccttcaacac gccgcgcatg gcccacgccg ccatgacccc
      181 cgacggcgcg ctcttcctcg acatttaggg acattcaggc cgccctcgcg gccggtggtc
      241 ccttactccg gccccacacc aaacgtccac cgttttttgc caaactgcaa cctccaggct
      301 accgggatat ttaggcccag gggctatact gataatgcgc gcggtgcgtg ccgcgcgcct
      361 caagctggct aatatttcgc tgccggaagg gcagcgtgcg cgaccgcagg tcgcaggaag
      421 aggtatctgt catgcaaccc aagattcttg ttcccctgga tggctcgccg ctggccgaga
      481 cggtgctgcc ccgcgcggcg gcctgggcgc gggccacggg cggtgtcgtc gtgctgacgc
      541 gggtgagcgc cccggctatg gtcgcgccgc cgacggcctg gacgatcccg acgcccctgg
      601 tcggctatga gaataccctc cacgatatgg acctggcgcg cgattatctg accgacgcgg
      661 cgatgcgcct gaagcagacc ggggtggagg tggagaccac cgggctggac ggcgacccgg
      721 cggcggcgat tatcgcctat gccgaggagc acccggaggt gacgctgatc gcgatggcga
      781 cgcacgggcg cagcgggctg agccgctgga tgctgggtag cgtggccgag aaagtgctgc
      841 acgccgcccc ggtcccgctg ctgatggtcc gccccaccgg gaacgacgcc ccggcgaccc
      901 cggcaagcga cctccagacg atcctcgtgc cgctggatgg ctcgatcttc gccgagcagg
      961 cgctggaagt ggcgtttgcg agcgcgcggg ccacgggcgc ggcgctgcgg ctgatcagcg
     1021 cgttgccggt gcccgacgac atggcggcca ccgatagcgg cctgaccccc gcgccggtgc
     1081 ccgcccagta ccagaccgag gtcgcgcacc tgacggccta cctggagcag caggcggaac
     1141 ccttgcgccg ggcggggatc agcgtcgaga cgcagatcgt caacgggcgc ccggcggacg
     1201 cgatcctgca tgccgccacg gaggcccacg ccgggctgat cgtgatggcg acgcacggac
     1261 gcagcgggct gcagcggctg tggctgggca gcgtggcgat gaaggtggtg cagggatcga
     1321 cgttgccggt gctgctggtg cgggcgcgcg aagccgccgt gcccgcggcc atccctgcct
     1381 gatttgcggc cggccggcgc gtatcccgct gctcttatgc tacgggcgcg ccagcgggtg
     1441 atccatagac atcgcgggca cggccccgcg ccacgagaag gaggtccaat ccctatgcaa
     1501 accgagattc tggttcccct tgacggctcg ctggtggcgg agacggcgct gccgccggcc
     1561 ctggccctgg cccgcgccct gggcgggtcg ctgaccctgc tgcgggtgac gccgccgacg
     1621 ctgggcgcgg agccgctggc cgaggctatc caggcgccgg tgatcaccta ccgtccgcgc
     1681 ccccagcatc gcgacgaggc cgacgcctac ctggccgggg tgacgcagcg gttcgcatcg
     1741 agcggcgtgc cgatgcgcat cctcgtgctg cggggtgatc ccgcgacggc gattttgcaa
     1801 tacgcgcagg aagcaccggg cgtgcgcctg atcgccatgg cgacgcatgg gcggagtggg
     1861 gtgagccgct gggtgttcgg cagcgtggcc gagaaggtgc tgcgcgcgac gccggcaccg
     1921 ctgctgctgg tgcgccccga cgacgacggc atggcgccgc cgcccatgca tgcgctgccc
     1981 taccgcacca tcatcgtgcc gctcgacggc tcgccgtttg ccgagcaggc cctggaccag
     2041 gcgtatgccg tggcggcggc cacgggtgcg agctagctgc tggtggcggt ggcccccgaa
     2101 cctgacgagt tcctggccgc cgaagtgggc gacatcccgg tctggcggct ggaagaccgc
     2161 ctggccgaga ccgagcgact ggacgcctat ctccaacagc aggccgcgcg gctcataaac
     2221 gccgggctga cagttgaaat tcaggtcagg gcggggagtc ccgcccaggc gattctggac
     2281 acagcggagg aagcggcggc ggacctgat
//
LOCUS       DASUYC010000727         6367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1034454, whole genome shotgun sequence.
ACCESSION   DASUYC010000727 DASUYC010000000
VERSION     DASUYC010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6367
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034454"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VF276_11890"
     CDS             complement(<1..215)
                     /locus_tag="VF276_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6607605.1"
                     /translation="MNRFSVLAGLALLIAGPMSSAAAGGVAQAPGPTTVSEGKYTVAG
                     LSGNYDLVNRIWDFPAGSWTPLHTHPGR"
     gene            complement(301..843)
                     /locus_tag="VF276_11895"
     CDS             complement(301..843)
                     /locus_tag="VF276_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607606.1"
                     /translation="MLENNVYLLERQMAAEVTTRRATAQRMHLAAPGRVASSGTVRSL
                     LVLGLALAGALALLALSTGPAHAAGLVGPTGAPVNGADAVYALVHVCAGATCARPADW
                     APAERQLQAQFQAGAWGRDTAVVSRAGAGQISWAQWAAQHRYYIEEPNASARRNAIRL
                     LRQELGNTVVVHIIPVPSTR"
     gene            993..3479
                     /locus_tag="VF276_11900"
     CDS             993..3479
                     /locus_tag="VF276_11900"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6607607.1"
                     /translation="MTTTPLAAVRWIKQRRKELGLTQEALAERAGCSWETVRKIEAGI
                     VRPSQQLAELLVVHLAVPPAERADAVRLLRAIPPTPKAALAPASAARNSRPLPIPPTS
                     LIGRAPEVARLRALLGRFEGRLITLVGPPGIGKTRLALAVAATLHDVFAGGVAFVELA
                     ALTDAALVPSTIARTLAVNEVTGQPLLDTLAAYLREKQMLLVLDNCEHLPAATPLVDT
                     LLRAAPGLKVLATSRTPLHVYGEKEFPVPALGLPDRQAVVSPEQAQWADAVHLFVERA
                     RDVQPDFALTAENAGAVAEICARLDGLPLAIELAAARIRLLPPAALAARLDHRLGLLT
                     GGARNLPARQQTLRGAIGWSYDLLDPAQRQLFRRLAVFQGGATLEAIAAVCNGAGDLP
                     VDVLDGVEALVENHLVQARVGRQDTPRFAMLETIHEYAADKLRESGEEAALRLVHARY
                     FLQLAEETDFVLGGAEQTIWLDRMEDEHNNHRAALAWLLNNTADEIGAKLELGLRLAG
                     ALGWLWDLRGFHSEGRRWLAIALERSGEHQQVAAQAVETPAFNKAVISAFASSALLAA
                     DQGEIETGRRFYERSLDISRKIDDKLNICKSLIGLGIMAATQGEAQAARAYYEECLVI
                     SREFGYKWGINQALNNLASLALEQGDYAAARAMYSESLSGRRESEGKFQVGQSLANLG
                     NVAYYQGDYTAARALVQEALAISLELGDKLGAVMGLAVMAGVEAQSGRLRKEQQDQAV
                     GLGIDGAADAEVIGTARRAAILIGVVDVVLETMGIVLDRVERRIYEQARAVTTELLDD
                     AAFAAARQEGRDMTLEQAVAFACALAQPGG"
     gene            complement(3533..4543)
                     /locus_tag="VF276_11905"
     CDS             complement(3533..4543)
                     /locus_tag="VF276_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6607608.1"
                     /translation="MKLLVLGGTVFLGRHIVEHALARGHEVTLFNRGRHNPDLFPDVE
                     KLRGDRDGDLGVLAGRRWDAAIDTSGYIPRVVRATATSLADAVDHYTFISSISVYADF
                     RTPHMDESAPVGTLADPTVEEVTGETYGPLKALCEQAVEDVLLGRALIIRPGLLVGPP
                     DPTDRFTYWPHRVVAGGEVLAPGRPEHAIQFIDARDLAAWIVRMVEAGQTGVYNATGP
                     ANPYTLGELLETCRVAAGNDAHFTWVPEAFLLEREVGPWMEVPLWVPEEEDNAGFDNV
                     DCSKALAAGLTFRPVLDTVRDTLAWDATRPADTERRAGLKPEREAELLTAWHANEGAP
                     TR"
     gene            4922..>6367
                     /locus_tag="VF276_11910"
     CDS             4922..>6367
                     /locus_tag="VF276_11910"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX6607609.1"
                     /translation="MRNDLDGTAPVRLAELIAALSLATDLGMGQPLEHALCSCVLAVR
                     LGEALGLDEAALREVYYQALLRYIGCNAETHTLAAVVGDELAMRRDFATIDNGNQGAV
                     LSLMLRYIRQAHAGASPLQLAREMARGLLAGREIQAGFAGHCEVAQRLAGRLGLDAPI
                     ITALGQLYERWDGRGLPRGLKGEEVAPAVLIVALAQDALTVRRLGGVDAAVAMTRQRS
                     GGAYDPRMVDCFCRAAPALFAGLDEAPSWETVLGMEPGARSYLAEDQLDTACQAIADF
                     ADLKSSYTLSHSSRVAELAAGAAQHCGLPAADVTAIRRAGWLHDLGRVGVSTGIWDKP
                     GALTETEWERVRMHPYYTERVLARSATLGRLGHLAAFHHERLDGSGYHRGAPAMILSP
                     AARILGAADAYQAMTEARAHRPARPPDAAAEELRREARAGRLDSDAVAAVLGAAGHHT
                     RPTRRDLVAGLSEREVEVLRLLGRGHSMKQIA"
BASE COUNT          967 a         2108 c         2309 g          983 t
ORIGIN      
        1 cggccggggt gggtatgcag cggcgtccag gacccggccg ggaagtccca gatccggttc
       61 accaggtcgt agttgccgga gaggcccgcg acggtatatt tgccctcgct gaccgtggtc
      121 gggccggggg cctgcgctac gccgcccgcg gctgcgctgc tcatcggccc cgcgatgagc
      181 agcgcgagcc cggccaatac gctgaacctg ttcatggtgt gtgcctcctt tgctagagtg
      241 gccccaaaaa aggccggcgc aggtgatgcc tgacgggttg ggcaccacct gcgctatcgg
      301 ctaccgcgta cttggcaccg ggatgatatg caccaccacg gtgttgccta gctcctggcg
      361 caacagccgg atggcgttgc gccgggcgct cgcgttcggc tcctcgatgt agtaccggtg
      421 ctgtgccgcc cactgcgccc aactgatctg gccggccccg gcgcgcgaca ccactgccgt
      481 atcccggccc caggcacccg cctggaactg cgcctgcagt tggcgctcgg cgggcgccca
      541 gtcggcgggg cgggcgcacg tggcgccggc acagacgtgg accagcgcgt agaccgcgtc
      601 ggctccgttg acgggggcac cggttgggcc gaccaatcct gccgcgtggg ccgggccggt
      661 cgacagcgcc agtagcgcga gcgcgcctgc tagcgccagc cccagtacca gcaagctacg
      721 cacggtcccg ctgctggcca cgcggcccgg cgctgcaagg tgcatccgct gcgcggtcgc
      781 gcggcgggtc gtgacttccg ccgccatctg ccgctccagc aagtagacat tgttctccag
      841 catcgctctg ctcctttcgt gctatgggcc gctcgccctt gcgtacaacc gttgaggcaa
      901 ttaaagcaga gaggctgatg gtaacagaag gtagaagtgg tatactaact ggtaggaaaa
      961 actggtagga aattgcgtcg ggagcccagg cgatgactac cacccctctg gcagccgtcc
     1021 ggtggatcaa gcagcggcgg aaggaactgg ggctaacgca ggaggcgctg gccgagcgcg
     1081 ccggctgctc ctgggagacc gtgcgcaaaa tcgaagccgg gatcgtgcgc ccctcgcagc
     1141 aactggccga gttgctggtg gtccacctgg ctgtcccacc ggctgagcgc gctgacgcgg
     1201 tgcgcctgct gcgcgccatc ccgcccaccc ctaaagccgc gctggcgcct gcgtcggccg
     1261 ctcgcaactc gcgcccactg cctatcccgc ccacttcctt gattggccgc gccccggaag
     1321 tagcccggct gcgcgcgctc ctgggccggt tcgagggccg gctgattacc ctcgtcggcc
     1381 cgccgggcat cggcaagacg cgcctggcgc tggccgtggc cgccacgctc cacgacgtgt
     1441 tcgccggcgg cgtcgcattt gtcgaactgg ccgccctgac cgacgcggcc ctcgtgccgt
     1501 ccacaattgc tcggaccctt gccgtaaacg aggtcaccgg ccaacccttg ctcgacaccc
     1561 tggcggcgta cctgcgcgaa aagcagatgc tgctggtgct ggataactgc gagcacttac
     1621 cggcggccac cccgctggtc gacacgctgc tgcgggcagc cccagggctg aaggtgctgg
     1681 ccaccagccg caccccgctg cacgtgtacg gcgagaagga gtttcccgtc cctgcgcttg
     1741 gcctgccgga tcggcaggcg gtagtatctc ctgagcaggc gcagtgggcc gacgcggtgc
     1801 acctgtttgt cgagcgggcg cgcgatgtcc aacccgactt tgccctgacg gcggagaacg
     1861 ccggggcggt cgccgagatc tgcgcccggc tggacgggct gccgctggcc atcgaactgg
     1921 ccgccgcgcg catccggctc ctgccgcccg cggcgcttgc ggcgcggctg gaccaccggc
     1981 tggggctgct gaccggcggg gcgcgcaacc tgccggcccg tcagcagaca ctgcgtgggg
     2041 cgattggatg gagctacgat ctcctggacc cggcccagcg gcagttgttc cgccggctgg
     2101 cggttttcca gggcggcgcc acgttggagg cgattgcggc cgtctgtaat ggcgcgggcg
     2161 atctgccggt agatgtgctg gacggcgtgg aggcgctggt agagaaccac ctggtgcagg
     2221 cgcgggtggg ccgccaagac acgccgcgct tcgcgatgct ggagacgatc cacgagtacg
     2281 ccgctgacaa gctgcgcgag agcggcgaag aagcggcgct gcggctggtc catgcccgct
     2341 attttctgca gttggccgag gagacggatt ttgtcctggg cggggcggag cagaccatct
     2401 ggctggacag gatggaagat gagcacaaca accaccgtgc ggctttggcc tggctactca
     2461 ataatactgc tgacgagatt ggggcgaagc tggaactggg cctccggctg gccggcgcgt
     2521 tgggctggtt gtgggaccta cgtggattcc atagcgaggg ccggcgatgg ctggcgatcg
     2581 cgctagagcg gtctggggaa caccagcagg ttgcagcaca agcagttgaa actccggctt
     2641 tcaataaagc cgtgatctca gcgttcgcct cgtccgcttt gttagccgcg gatcaaggcg
     2701 agatcgagac aggtcgccgc ttctacgagc ggagtctgga tattagccgc aagatcgatg
     2761 ataaactgaa catatgcaag tctttaatcg gcctgggaat catggctgcg actcaaggcg
     2821 aggcccaggc ggcccgagca tactatgagg agtgcctggt gatcagccgg gaattcggat
     2881 acaaatgggg tatcaatcaa gcactgaata atctggccag cctagccctt gagcaagggg
     2941 attacgcagc ggcgcgcgcc atgtacagtg agagcttgtc tggcaggcgg gagagcgagg
     3001 gcaagttcca ggtcggccag tcgttagcca accttgggaa tgtcgcgtac taccaggggg
     3061 attacactgc tgctcgtgct cttgtacagg aggccctagc gataagcttg gaactggggg
     3121 acaagctggg ggcggtaatg ggattagccg tcatggccgg agtggaggcc cagagtgggc
     3181 gcttgcgaaa ggagcagcaa gatcaggcgg tagggcttgg aatcgatggc gcggcggacg
     3241 ccgaggttat agggacggca agacgggccg ctatactgat tggcgttgta gacgtggtac
     3301 tggaaaccat ggggatagtt ttggaccggg tggagcgccg catatatgaa caggcgcgcg
     3361 ccgtgaccac ggagttgttg gatgacgccg ccttcgcagc agcgcggcag gaggggcggg
     3421 acatgacgct ggagcaagcg gtcgcctttg cctgcgccct tgcgcaaccg ggcggctgag
     3481 gaagtggggt tcgcggcgcg gcgcatggac cggcaccgcg ccgcgggggc cgtcagcggg
     3541 tgggggcgcc ctcattggcg tgccaggcgg tcagtagttc ggcttcgcgc tcgggtttga
     3601 ggccggcgcg gcgctcggtg tccgccgggc gagtggcgtc ccaggccagc gtgtcgcgca
     3661 ccgtgtcgag caccgggcgg aaggtcagcc cggcggcgag cgccttgctg caatcgacgt
     3721 tgtcgaagcc ggcgttgtcc tcctcctccg gcacccagag cggcacctcc atccaggggc
     3781 cgacctcgcg ctccagcaag aacgcctcgg gcacccaggt gaagtgggcg tcgttcccgg
     3841 cggccacacg gcaggtctcc agcaactcgc ccagggtgta gggattggcc gggccggtgg
     3901 cgttgtagac tccggtctgc ccggcctcga ccatgcgcac gatccaggcg gccaggtcgc
     3961 gggcgtcgat gaactggatc gcgtgttcgg ggcggcccgg cgctagcacc tcgcccccgg
     4021 cgaccacgcg gtgcggccag taggtgaagc ggtcggtcgg gtcgggcggg ccgaccagca
     4081 gaccgggccg gatgatcagc gcgcgcccca gcagcacgtc ttcgacggcc tgctcgcaca
     4141 gcgccttgag cggaccgtag gtctcgcccg tcacttcttc caccgtggga tcggccagcg
     4201 tgcccaccgg ggcgctctcg tccatgtgcg gcgtgcgaaa atcggcgtag accgagatgc
     4261 tggagatgaa ggtgtagtgg tcgacggcat cggccagcga ggtcgccgtg gcgcgcacca
     4321 cgcgcgggat gtagcccgac gtgtcgatgg cggcatccca ccggcgcccg gccaggacgc
     4381 ccaggtcgcc gtcgcggtcg ccgcgcagct tctcgacatc cgggaagagg tcggggttgt
     4441 gccggccccg gttgaagagg gtcacttcat ggccgcgggc cagggcgtgt tcgacgatat
     4501 ggcggcccag gaacacggtg ccgcccagca ccagtaattt catgcgcatc ctcctgcgaa
     4561 tgatctcgcg gcgcgtggac accgccgtgt cgatccgccc ccagtgagcg tgtgccgggc
     4621 cgggggcctc cgcagtatag catatggccc ggtccggtgt cccatgtggc agtgcgcggt
     4681 gcggtaagcg cgggcgcgcg atagcgagag cgcccggccc gtcaacgggc gggctggata
     4741 gaagagacag gccgcgcagg cggccttcgt aggggtagcg ggcggcttga gccgcccggc
     4801 gccccgcttg cacgccgccc ggcaatcgag taggatcagc agtagacagc aggggccttt
     4861 ccgcggcaga gtgacagcgg ccccggcgac tcccgccggg ggcgatcggc ggaggatcag
     4921 catgcgaaac gacctggacg gcaccgcgcc tgtgcgcctg gcggaactca tcgccgccct
     4981 gtccctcgcg accgacctgg gcatgggcca gccgctggaa cacgcgctct gctcatgcgt
     5041 gctggccgtg cgcctgggcg aggccctggg gctggatgag gcggccctgc gcgaggtcta
     5101 ttaccaggcc ctgctgcgct acatcggctg caacgccgag acccataccc tggccgccgt
     5161 cgtgggcgat gaactggcga tgcgccggga cttcgcgacc atcgacaacg gcaaccaggg
     5221 cgccgtgctc agcctgatgc tgcgctatat ccgccaggcc cacgcgggcg cgtcgccgct
     5281 gcaactggca cgcgagatgg cccgcggcct gctggccggg cgggaaatcc aggcagggtt
     5341 cgccgggcat tgtgaggtgg cgcagcggct ggccgggcgg ctcggcctcg acgcgccgat
     5401 catcacggcg ctgggccagt tgtacgagcg ctgggatggg cgtggcctgc cgcggggcct
     5461 caagggcgag gaggtcgccc ccgctgtgct catcgtggcc ctggcccagg acgcgctcac
     5521 cgtgcgccgg ctgggcgggg tggatgccgc ggtcgccatg acccggcagc gcagcggcgg
     5581 cgcctacgac ccgcgcatgg tggactgctt ctgccgggcg gcccccgcgc tgttcgccgg
     5641 gctggacgag gcgccatcct gggagacggt gctggggatg gagccgggcg cgcgcagcta
     5701 tctggccgag gaccagttgg ataccgcctg ccaggccatc gccgatttcg ccgatctcaa
     5761 atcgtcctac acgctcagcc attcgagccg ggtggccgag ctggccgccg gggcggcgca
     5821 gcactgcggg ctgcccgccg ccgatgtgac ggcgatccgc cgggccgggt ggttgcacga
     5881 cctgggccgg gtcggcgtct cgaccggcat ctgggacaag cccggcgcgt tgaccgagac
     5941 cgagtgggag cgcgtgcgca tgcacccgta ctacacggag cgggtgctgg cgcgctcggc
     6001 cacgctgggc cgcctgggcc acctcgccgc cttccaccat gagcgactcg acggctcggg
     6061 ctaccaccgc ggcgccccgg cgatgatcct gtcgcctgcc gcgcgcatcc tcggcgccgc
     6121 cgacgcctac caggccatga ccgaggcccg cgcccaccgc ccggcccgcc ccccggacgc
     6181 cgctgccgag gaactccgcc gcgaggcccg cgccgggcgg ctcgacagcg acgccgtcgc
     6241 ggcggtgctc ggcgcggcgg gccaccacac gcgccccacg cgccgcgacc tggtggccgg
     6301 gctgagcgag cgcgaggtcg aagtgctacg cctgctgggc cgtggccact cgatgaagca
     6361 gatcgcc
//
LOCUS       DASUYC010000728         3518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1883527, whole genome shotgun sequence.
ACCESSION   DASUYC010000728 DASUYC010000000
VERSION     DASUYC010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1883527"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VF276_11915"
     CDS             complement(<1..434)
                     /locus_tag="VF276_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEX6607610.1"
                     /translation="MAIFDLPLDELRQYRPPRNEPADFDAFWATTLAAARAFPLDAQF
                     APVDYGLRTVETFDLTFSGYGGQRIKGWLLLPRNRAGRLPCVVEYIGYGGGRGLPTDW
                     LLWSGAGYAHLVMDTRGQGSSWLQGDTPDAEPDGGNPHYPGF"
     gene            1008..2153
                     /locus_tag="VF276_11920"
     CDS             1008..2153
                     /locus_tag="VF276_11920"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEX6607611.1"
                     /translation="MNRRYLLLCAAALGLGLLCLAPVGPAAAQEESRYFPETKHTVRG
                     LFLKYWTEHGGLAQQGYPLTEEFQEQSKLNGQVYTVQYFERAIFERHPENQPPYDVLL
                     SQLGTYELDARYPNGANPAAAPVSQAPGADSRYFPETKHSVSGLFLKYWTEHGGLAQQ
                     GYPLTEPFQERSALNGQTYTVQYFERAIFEHHPENAGTPYEVLLSQLGKFQLDTRYPN
                     GGTPTATPVLTGTPTATPTPLADAATPTVTATPMPTQVGTNCEPVAENLKAPVAHTGD
                     VFISNVEAAGLEYVEIRNLGTANANLGGWTLRDKNEVRQHYIFDEGAVLPGNMSLRVY
                     TEPGHPYTFNSRRAIWNNCGDALELLDAAAHVVSTYGYGTHCAANCE"
     gene            2426..3289
                     /gene="cpaB"
                     /locus_tag="VF276_11925"
     CDS             2426..3289
                     /gene="cpaB"
                     /locus_tag="VF276_11925"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEX6607612.1"
                     /translation="MLLLGVIFALLAFVVLYLALSRPSEPTVAQEPTPVPKAQVVVAA
                     RDIKGFTPITAEDVTLKDMEVTQILTPTLTSGQMVIGKMVTRDYKANDQIIATDVAEL
                     GISQILVKGQKAFVLPVKEINNFGGQIVDGDVVDVLWTRTFELTTLLVGPDGKPSQVT
                     KPLPSTKKVLDNIKILRVIQLRAAPTNKQGSGPVNPQGGQQPSDQQAQATAQQAAQAA
                     YSPDALVTAALVLSVTDQQAEVIKYARETGVVDLTLRARDDTDVERTTGITDKIMVED
                     YGVQIPELIVK"
BASE COUNT          689 a         1136 c         1112 g          581 t
ORIGIN      
        1 atgaagccgg gatagtgcgg gttgccgccg tccggctcgg cgtcgggcgt gtcgccctgc
       61 aaccaggaac tgccctgccc gcgcgtgtcc atcaccaggt gggcatagcc cgcgcccgac
      121 cacagcagcc agtcggtcgg caggccccgc ccgccgccgt agccgatgta ctcgaccacg
      181 cagggcaggc gccccgcgcg gttgcgcggc agcagcagcc agcccttgat gcgctgcccg
      241 ccgtagccgc tgaaagtgag gtcaaacgtc tcgaccgtgc gcaggccgta gtccaccggc
      301 gcgaactgcg cgtcgagcgg gaacgcacgc gctgcagcca gggtggttgc ccagaaagcg
      361 tcgaaatcgg ccggttcgtt gcgcggcggg cggtactgcc gcaactcgtc aagcggcaga
      421 tcgaaaatag ccatatcact ccccctgcga tggattgctg cggtgccctg gcgggccgca
      481 ctccggcgat gatagcacca gcccgccgct ccgtcaaccg gcggccctga ctgccctcat
      541 agaggcagcg cgaaggcggc aagatcgcga agaacgcgat taggaaaaac gatcttcgtg
      601 gtttatgcgt ctttgcggtg cttctatgag cggctggata gcccggctat aggcaggcca
      661 ggggtcacaa tcgtgtacca tttcgacggt gcccggcagg ggatcgcagc gttctgatgt
      721 agaagatgaa agcgcgaata gccctaacct taacgctctc tgcgtccctg cggtgaggat
      781 caatcggcgc tggacggtgg gcatgggccg ttttgacggg ccgggcggcg tgtgctaaaa
      841 taagcccgca acagtggtaa ttagctggat gggcgcggga ctcgcggatt gtgctgcccg
      901 gatcgccccc gccgatccac acgcggccta cacggtgtgc ctcgtggaaa tgccccattg
      961 cgcctttgcc tcgcgtgccc cgcctatggt atggtaagga gaatcgcgtg aaccgacgtt
     1021 atctgctgct ctgcgccgcc gccctgggtc tggggttgct ttgcctggcc cccgtggggc
     1081 cggccgccgc ccaggaggag agccgctact tcccggagac taaacatacc gtgcgcgggt
     1141 tgttcctgaa gtactggacg gagcatggcg ggttggctca gcagggctac ccgctgacgg
     1201 aggagttcca ggagcagtcg aagctgaacg gccaggtcta cacggtgcaa tacttcgagc
     1261 gcgccatctt cgagcgccac cccgagaacc aaccgccgta tgacgtgctg ctgagccagt
     1321 tgggcaccta tgaactcgat gcgcgctatc ccaacggtgc caatcccgcg gcggccccgg
     1381 tcagccaggc gccgggcgcc gacagccgct acttcccgga gaccaaacat agcgtgtcgg
     1441 gcctgttcct caagtactgg acggagcacg gcgggctggc gcagcagggc tacccgctga
     1501 ccgagccgtt ccaggagcgg tccgcgctca acgggcaaac ctacaccgtg cagtatttcg
     1561 agcgcgcgat tttcgagcac catccggaga acgccggcac accctatgaa gtgctgctga
     1621 gccagttggg caaattccag ctcgacactc gctatccgaa cggcggcaca cccacggcca
     1681 cgccggtgct tacgggcacg cccacggcca cgcccacccc cctcgcggat gccgccacgc
     1741 ccaccgtgac cgccacgccc atgcccaccc aggtcggcac caattgcgag ccggtggccg
     1801 agaacctcaa ggcgcccgtc gcgcatactg gcgacgtatt catcagcaat gtggaggcgg
     1861 cgggcctgga atacgtcgag atccgtaacc tgggcacggc taacgccaat ctagggggat
     1921 ggacgctgcg cgacaagaac gaggtccgcc agcactacat cttcgacgag ggtgccgtgt
     1981 tgccgggcaa catgagcctg cgcgtctaca ccgagccggg ccacccgtac accttcaaca
     2041 gccgccgcgc gatctggaac aactgcggcg acgcgctgga attgctcgac gccgccgccc
     2101 acgtcgtctc gacctatggc tacggcactc attgcgcggc caactgtgaa tgatcgtcgc
     2161 gcggggggcc gccagggacc atagtccctg aaacgacacc aatagtacta tatgaatcca
     2221 ggaccactaa cccctggtcc tgggccttac gctgtggtaa tctagagtgg cagttatcgc
     2281 gtagtgcaga gtacgtgtgt tgcatgttgg ctgggccggt gctataatgt tcacacacaa
     2341 tagcagtcgt gacggctgac aaccatcccc gccgcgaatg cgggtaggcc agggaggaat
     2401 gtgaatgagg cgtggccgga gtacgatgct acttctgggt gttatcttcg cattattagc
     2461 tttcgtcgtt ttgtatctcg cgctttcgcg tcccagcgaa ccgactgtag ctcaagagcc
     2521 gactcccgtt cccaaggccc aggtcgtggt ggccgcgcgg gacattaaag gctttacccc
     2581 aattaccgct gaggatgtga ccctcaagga catggaagtc acccagatcc tgacgccgac
     2641 tctgacctca ggccagatgg tgatcggcaa gatggtgacg cgggactaca aggccaacga
     2701 ccagatcatc gccaccgacg tggccgagct gggcatctcg cagatcctgg tgaagggcca
     2761 gaaagcgttt gtcctcccgg tcaaggagat caacaacttc ggcgggcaga tcgtggacgg
     2821 ggatgtcgtg gatgtgctct ggacgcgcac cttcgagttg accacgctgc tggtgggacc
     2881 cgacggcaag cccagccagg tcacgaaacc gttgccgtcg accaaaaagg tgctcgacaa
     2941 catcaagatc ctgcgggtga ttcagttgcg ggcggcgccc accaacaaac agggcagcgg
     3001 cccagtcaac ccgcaaggcg gccagcaacc gtcggatcag caggcgcagg ccaccgcgca
     3061 gcaggccgcc caggccgcct atagcccgga cgccctggtc accgcggcgc tggtcctctc
     3121 cgtgaccgac cagcaggccg aagtcatcaa gtacgcgcgc gaaacgggcg tggtcgacct
     3181 gaccctgcgc gcgcgcgatg ataccgatgt cgagcgcacg accggtatca ccgacaagat
     3241 tatggtcgaa gactacggcg tgcagatacc ggaactcatc gtcaagtagc gccccggccg
     3301 ggggcctctg tccagggatg gctcacgcag cggcggctgt agacgcgctc cgggcgcggc
     3361 agccgcgcgg cgggagccgc ttccctatcc agcgcaacgc ttaaccacgc taaaagagga
     3421 aagacatcaa tgtcgagcga accccgaatc cgccttgtga tcgtcgacga taacgtggac
     3481 gcgcgcgatc agatgagcaa gctcgtcatg ttcgagcg
//
LOCUS       DASUYC010000729         3056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_341657,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000729 DASUYC010000000
VERSION     DASUYC010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341657"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /locus_tag="VF276_11930"
     CDS             <1..1143
                     /locus_tag="VF276_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX6607613.1"
                     /translation="IAASILGYKTVFVMPDKVSGEKIALLRAFGAEVITTPTAVPRES
                     PESYYSVAARVTSETPGAFQPNQYFNQANPQTHYETTGPEIWEQTGGKVDVFVAGVGT
                     GGTITGIGRYLKERNPNVMIVGADPEGSIYTGDKPRPYKVEGIGEDFMPGTFQYDIVD
                     RWVQVSDRDSLLTARRITREEGILVGGSTGTAMWAALQVAKDLDESKTIVVLFPDTGR
                     GYLSKVFNDDWMRENGFLDRLATPARVRDLLHYHNRELPALVTVPTTARVREAIELLH
                     QYSISQLPVSRDGDLTTVHSIVGSIQERSLLERVFRNPEAVDQTVETVMEQPFPLVDV
                     GEEVERVVPALLAGNPAVLAEEAGRPVGLITRADLLEFVARGKTAQ"
     gene            1196..1714
                     /locus_tag="VF276_11935"
     CDS             1196..1714
                     /locus_tag="VF276_11935"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX6607614.1"
                     /translation="MDAVTLLREQISTAHEWLEGTMADVTAEQADWAPPGIANPLGAS
                     YAHAVTSEDLIINGMLGGGTPLFMGEWAGKTGQSLPHPHQDWTQYAAWARDVRVDLPA
                     ARAYAQAVYAATDAYLAGLTPEDLDREVNLFGMNQRLGMALANFVAGHCHDMMGEISC
                     LKGLQGVRGYPF"
     gene            1844..2986
                     /locus_tag="VF276_11940"
     CDS             1844..2986
                     /locus_tag="VF276_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003270948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX6607615.1"
                     /translation="MNKQYQVHLTAPEITQLQQLLRRGTHSARVQTRARILLLAHAGQ
                     TDAAIAAALLTSTATVQRVRQRAVQDGVAAALAERPRPGRPPRLTGDGEAHLTVLACT
                     APPAGRARWTIQLLTDHIALCEEAPAVSRETVRRRLHAHALKPWQHKQWCIPQAGARF
                     VAKMEDVLGVYARPYDPARPVVCLDEANKTLHADSRLGQPMAPGPGARQDYEYERNGT
                     ANLFVWVEPLAGRRGVQVTAQRTTPDFATVVRHLVDEVYPHAAQIVLVTDNLNTHGPW
                     ALYETFAPAEAARVLAKLDWHCTPEHGSWLNMAEIEIGALRRQCLARRIATPTELAAE
                     VAAWVAERNAQGGTIDWQFTTAEARIKLKRLYPVLKPPTRVFTSQN"
BASE COUNT          525 a         1076 c          996 g          459 t
ORIGIN      
        1 attgccgcgt ccatcctggg ctacaagacc gtgttcgtca tgcccgacaa ggtcagcggc
       61 gagaagatcg ccctgctgcg cgcgttcggc gccgaggtga tcaccacgcc caccgccgtg
      121 ccgcgcgaaa gcccggagag ctactacagc gtggccgccc gcgtcaccag cgagaccccc
      181 ggcgccttcc agcccaacca gtacttcaac caggccaacc cccagacgca ctacgagacc
      241 accggccccg aaatctggga gcaaaccggc ggcaaggtgg acgtgttcgt ggccggcgtg
      301 ggcaccggcg gcaccatcac cggcatcggg cgctacctca aggagcgcaa cccgaacgtg
      361 atgatcgtcg gcgccgaccc cgaaggctcg atctacaccg gcgacaagcc gcgcccctac
      421 aaggtcgagg gcatcggcga ggacttcatg cccggcacct tccaatacga catcgtggac
      481 cgctgggtgc aggtgagcga ccgcgactcg ttgctgaccg ctcgccgcat cacccgcgag
      541 gaaggcatcc tcgtcggcgg ctcgaccggc acggcgatgt gggccgcgct ccaggtggcg
      601 aaggacctcg acgagagcaa gaccatcgtc gtgctcttcc ccgacaccgg gcgcggctac
      661 ctgagcaagg tcttcaacga cgactggatg cgcgagaacg gcttcctgga ccggctggcg
      721 accccggccc gcgtgcgcga cctgctgcac taccacaacc gcgagttgcc cgccctcgtc
      781 accgtgccga cgacggcccg cgtgcgcgag gccattgaac tgctgcacca gtacagcatc
      841 tcccagttgc cggtgtcgcg cgacggcgac ctgaccaccg tgcattcgat tgtcgggtcg
      901 atccaggagc gctcgctgct ggagcgggtc ttccgcaacc cggaggctgt ggaccagacg
      961 gtcgagacgg tgatggagca gccgttcccg ctggtcgacg tgggcgagga agtggagcgc
     1021 gtggtgcccg cgctgctggc cggcaacccg gcggtgctgg ccgaggaagc gggccgcccc
     1081 gtcggcctga tcacccgcgc cgacctgctg gagttcgtgg cccgcggcaa gaccgcgcag
     1141 taattcgccg ccgccggcca cccgatcgcg gcgccggggc taaaggagat ttgccttgga
     1201 tgctgttacc ctcttacgcg agcagatcag cacggcccac gagtggctgg aaggcacgat
     1261 ggccgacgtg accgccgagc aggccgactg ggcgcccccc ggcatagcca atccgctggg
     1321 ggccagctat gctcacgcgg tcacgagcga ggatttgatc atcaacggca tgctgggcgg
     1381 cggcacgccg ctgttcatgg gcgagtgggc cggcaagacc ggtcagagcc tgccccaccc
     1441 ccaccaggat tggacgcagt atgcagcgtg ggcgcgcgac gtgcgggtgg acctgcccgc
     1501 ggcccgcgcc tacgcccagg cggtctacgc cgccaccgat gcctacctgg ccgggctgac
     1561 ccccgaagac ctggaccgcg aggtcaacct gttcggtatg aaccaacgcc tcggcatggc
     1621 cctggcaaac tttgtcgccg gtcactgtca cgatatgatg ggcgagatct cgtgcctgaa
     1681 ggggttgcag ggcgtccgcg gctacccatt ctagtcgccc acggtaggat ctgggtgtcg
     1741 ctctcgcgcg gcggccaggg ttgcagggcg tgcgcggcta cctgttctag tactttaaca
     1801 ccttaatttt gcgaagttgc gggtgggttg gtagactctg ggcatgaaca aacagtacca
     1861 ggtccacctc accgcgcccg aaatcaccca gctccagcag ctcctgcgcc ggggcaccca
     1921 ctcggcgcgc gtccagaccc gtgcgcgcat tctgctgctc gcccatgccg gccagaccga
     1981 cgccgccatt gccgccgccc tgctgaccag cacggccacg gtgcaacgcg tgcgccagcg
     2041 cgctgtccaa gacggggtcg ctgccgccct cgccgaacgc ccccgccccg gccgcccgcc
     2101 ccggctcacc ggcgacggcg aagcccacct gaccgtgctg gcctgcaccg cgccccccgc
     2161 cgggcgggcg cgctggacga tccaattact caccgaccac atcgcccttt gcgaagaggc
     2221 cccggccgtg tcgcgcgaaa cggtgcgccg ccgcttacac gcccacgcgc tcaagccctg
     2281 gcagcacaag cagtggtgta tcccccaagc cggcgcgcgg tttgtggcca aaatggaaga
     2341 tgtcctgggc gtctacgccc gcccgtatga tccggcccgc cccgtggtct gtctggatga
     2401 agccaacaag accctgcacg ccgatagccg cctcggtcaa ccgatggcgc cgggccccgg
     2461 ggcgcgccag gattatgagt atgagcgcaa cggcacggcc aatctgttcg tctgggtcga
     2521 accgctggct gggcggcgcg gggtgcaagt aacggcccag cggaccacgc cggactttgc
     2581 caccgtggtg cgccacttag tggacgaggt ctacccgcac gccgcgcaga tagtgctggt
     2641 gaccgataac ctgaacacgc acgggccgtg ggccttgtac gagacctttg cgccggccga
     2701 agccgcgcgg gtgctggcga aactcgactg gcattgcacg ccggaacatg gcagttggct
     2761 gaacatggcc gaaattgaga tcggggcact ccggcgccag tgcttagcgc gccggatcgc
     2821 gacgcccacg gaactcgccg cggaagtagc ggcctgggtt gcggagcgca acgcgcaagg
     2881 cggcacgatt gactggcagt ttacgacggc cgaggcccgc atcaagctca agcgcttgta
     2941 tccggtgctg aagcctccga cgcgcgtgtt tacttcgcaa aattaagttg ttaaagtact
     3001 agtcgcccac cccagtgatt ctgctgggcg ggcaggccgg tttcaaaccg gcccct
//
LOCUS       DASUYC010000730         6770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_341951,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000730 DASUYC010000000
VERSION     DASUYC010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6770
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341951"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..819
                     /locus_tag="VF276_11945"
     CDS             103..819
                     /locus_tag="VF276_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607616.1"
                     /translation="MTMKVNELVSYVFSGSTYSFAGPFTRWLEASPPFRAFVEVHRDK
                     IRKKVRNIRDDDTQRDVVAELAVARWLAGERRFAVAYEVYIAGKQRGPDFSVTFRTRI
                     PFNVEVTRLRPGGTGSAGEDTAPRTKIANTICAKLAQFPSSVINVLVLVTDGAAYGAD
                     DLAGAPGVLAERALRKDDAFFQRRGLLGTRNFHHHYQRLSAILLRRLTETGALEPVAL
                     WANPQARHPLPADLATILRQ"
     gene            complement(836..1012)
                     /locus_tag="VF276_11950"
     CDS             complement(836..1012)
                     /locus_tag="VF276_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607617.1"
                     /translation="METVQELEILGSLVLIVAAVATLLVVAPLHPGSALAPVALAVLG
                     LGFVGLRHALRTRP"
     gene            1167..2267
                     /locus_tag="VF276_11955"
     CDS             1167..2267
                     /locus_tag="VF276_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX6607618.1"
                     /translation="MPESKLRVGVIGAGIGTLHLAAYRDLPRVEIAALAGLDDDRVRA
                     AAEKYGVPQTYRHYEDLLARRDIDAVSICLPTVLHAPVTMAAVESGKHVLVEKPLAMT
                     PAEGRAMIAAAEAHRRILMITFNHRYRGDVQWVKRYVDSGALGRIYYAKAHWMRRAGI
                     PRLGSWFGNQAQAGGGPLIDLGVHVLDMALYLMNEPRVRAVSAATYAEFGPRGLKGAG
                     PALVPVGSEGLPYDVEDLATAFLRLDGGGTLLLEASWATHSAASDDFGVTLYGSEAGV
                     ELLVRDYSYQNTVRVFSDVAGVPADLTPRIPSGGGHQAVVARFVDAILDGAPAVPSAA
                     DGLRRAEIIAACYESAAAGAEVPILPGVASVP"
     gene            2421..3149
                     /locus_tag="VF276_11960"
     CDS             2421..3149
                     /locus_tag="VF276_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEX6607619.1"
                     /translation="MSNPLRVTVWNEYRHEQNSGHPASRVYPHGIHEAIAAYLREQPG
                     LEVRTATLDAPDQGLPAEALDTTDVLIWWGHDAHDAVADALVDRIQARVLAGMGLVVL
                     HSGHFSKIFKRLMGTSCNLQWREGNDHERLWVVEPGHSIAAGLPPYIDLAHEEMYGER
                     FDIPAPETLVLVSWFSGGEVFRSGCCYTRGLGKIFYFRPGHESFPTYYDPLVRQVIGN
                     AVRWAAPAGGTPPVFGNVPPREQQ"
     gene            3176..4591
                     /locus_tag="VF276_11965"
     CDS             3176..4591
                     /locus_tag="VF276_11965"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX6607620.1"
                     /translation="MATRLPPHPPRLPGVGTLFPSMRDPIGYPIRNYRRYGEVVGVHL
                     LNVHGAALYGPAANRYILVDNVENFLVEPLVDRLGVRLLVGEGVLFIDDPRHRKQRRL
                     IMPAFHRKRIENYQEVMRQETQAMLDSWTPGVALDVAAEMRRLALVIAGRTLFSMDLG
                     GNASELGVAVATLVGTLGSVFNATLARVPFNLPLLGGRGGSVRAGLARIDRVLGAIIA
                     EHEREGTDTGDVVSMLVAARDEDGSRLSPKQIRDHLLTLFVAGHETSANALAWTFYLL
                     AQHPDVTARLLAELDRELHGAAPTPADLERLPYLEQVIKESMRLYPPAPSAVRTAREG
                     FEWQGYTIRAGEVVFYSPYISHRMPHIFPEPETFRPERFAPDSPENPPPYAYIPFAAG
                     PRSCIGAPFAMMEIKTVLAMTLPRFRMDLVRGQRVDQAMRLTLQPKYGIQMTPQPQDG
                     HPERSRARVLGNAVGATPGPP"
     gene            complement(4701..5297)
                     /locus_tag="VF276_11970"
     CDS             complement(4701..5297)
                     /locus_tag="VF276_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607621.1"
                     /translation="MIVENQATATEAARVYHLRHMYLYAGGFLLLCLGLAGFMLWLPL
                     SMDEPLDTASQVVIIVGAAILSALGIACCVAFLNIRLVAGPAGIAYYSWGYRLRTTWD
                     NIEGTGELWTTRGLIRGLQLREPALDMDGWMRIGRGAIPVMSTIGALSGRGLPRSGSM
                     AGYDRIIPVGYFAQDWETSDLGALIRRYAPQALDPAPK"
     gene            complement(5661..6710)
                     /locus_tag="VF276_11975"
     CDS             complement(5661..6710)
                     /locus_tag="VF276_11975"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6607622.1"
                     /translation="MTILAVEIRRLDPQTATGAEWRALNALNNILEAEAFPDDPPEKL
                     ETTIKYVRDIPAYADVRRWHAWQGDEIVGRGVFSIWSPNDNLHMADFWIGVRPDMRRQ
                     GIARRLLAPVVAAAEQDQRRLLVTWGTDRVPGTDAFLNRLGAHMGLETHTNQLLIADV
                     DRDQLRAWQERARERAPEFQLGFWEGAHPEELIPAMIEARKGMNLAPIGTIEWEDHIT
                     TPEELREQDASRARRGIIRWTMYVREGDSGPIAGYTEVFWDPQYPERLWQEETTVLPP
                     YRNRGLGRWIKAAMLEKVIRERPQIRYVRTGNADSNGPMLKINYDLGYKPYMAERVWQ
                     VETAQVRAYIGPDAE"
BASE COUNT         1057 a         2195 c         2399 g         1119 t
ORIGIN      
        1 cggtatgcgc cgtgctgtgc tcgtcctacc ggcaccgccg gccttcttag gcgtagcccg
       61 gtatctgcga ttcgctctag cgatccggtg gagatggtga acatgacgat gaaggtcaac
      121 gagcttgtga gctacgtctt cagcggcagc acctacagct tcgcgggacc gtttacccgc
      181 tggcttgagg cctcgccgcc ctttcgcgcc ttcgtggaag tgcatcggga caagatccgc
      241 aagaaggtgc gcaatatccg ggacgacgac acgcaacggg acgtggtggc cgaattggcc
      301 gtcgcacgct ggttggcggg ggagcgccgg ttcgcggtgg cctatgaggt gtatatcgcc
      361 ggaaagcagc gcgggcccga tttttcggtg acgttccgca cgcgcatccc gttcaacgtg
      421 gaagtcaccc ggctgcgccc cggcggcacc ggctcggcgg gcgaggacac ggccccccgc
      481 accaagatcg ccaataccat ctgcgccaaa cttgcccagt tcccgtcgag tgtgatcaac
      541 gtgctggtgc tggtcacgga cggggcggcc tatggggcgg acgacctggc gggggcgccg
      601 ggcgtgctgg ccgagcgcgc cctgcgcaaa gacgatgcct tttttcaacg tcgcgggttg
      661 ctcggcacgc gcaacttcca tcaccactac cagcggctca gcgcgatcct cttgcgccgc
      721 ctgaccgaga ccggcgcgct ggaaccggtg gccctgtggg ccaatccgca agcccgccat
      781 ccgctacccg ccgacctggc gacgatcttg cggcagtgac gcgcgccggg caggctcacg
      841 ggcgggtgcg cagcgcgtgg cgcaggccca cgaagccgag gcccagcacc gccagcgcga
      901 ccggcgccag ggcgctgccg gggtgcagcg gggccacgac cagcagggtc gccaccgccg
      961 cgacgatcag taccaggctg cccaggatct ccagttcttg tacggtctcc attgtgctgt
     1021 ctccctggct tgcagatgta tcatagtggg aacggcgccc ggccccgccg gattacagac
     1081 gccgccttgc catctccggt acaatagtac gggaccgcgg ggccggtggt cacagcccgc
     1141 gatcacttcc agcaagggag aaccgtatgc ctgagagcaa actccgcgtc ggcgtgatcg
     1201 gcgcgggaat cgggacgctg cacctggcgg cgtacaggga cctgccgcgg gtggaaatcg
     1261 ccgccctggc cgggctggac gacgaccggg tgcgtgcggc agcggagaaa tatggcgtgc
     1321 cgcagaccta ccgccattat gaagacctgc tcgcgcggcg cgatatcgac gcggtgagca
     1381 tctgcctgcc gacggtactg cacgcgccgg tgacgatggc cgccgtggag agcggcaagc
     1441 atgtgctggt cgagaagccg ctggcgatga cgcctgccga gggccgggcc atgatcgccg
     1501 ccgccgaggc tcaccgccgc atcctgatga tcacgttcaa ccaccgctat cggggcgatg
     1561 tgcagtgggt caagcgctac gtcgatagcg gggcgctggg ccggatttac tacgccaagg
     1621 cccactggat gcgccgggcc gggatccccc ggctgggaag ctggttcggc aaccaggccc
     1681 aggcgggcgg cgggccgctg atcgacctgg gcgtgcatgt gctcgacatg gccctctacc
     1741 tgatgaatga gccgcgggtg cgcgcggtca gcgccgccac ctatgccgag ttcgggccgc
     1801 gcgggctgaa gggtgctggc ccggccctgg tgcccgtggg gtccgagggg ctgccgtacg
     1861 atgtcgagga cctggcgacg gccttcttgc ggctcgacgg cgggggcacg ctgctgctcg
     1921 aagcgagttg ggccacgcac agcgccgcga gcgacgactt cggcgtgacg ctctacggca
     1981 gcgaggccgg cgtcgaactg ctggtccgcg actacagcta tcaaaacacc gtgcgcgtgt
     2041 tcagcgatgt cgcgggcgtg cccgccgact tgacgccgcg catcccatcg ggcggcgggc
     2101 accaggccgt ggtcgcccgg ttcgtcgacg cgatcctgga tggggcgccc gccgtgccca
     2161 gcgccgccga cggcctgcgc cgcgccgaga tcatcgccgc ctgctacgag tccgcggcgg
     2221 cgggcgccga agtgccgatt ctccccggcg tggcaagcgt cccctgagcg gcgttccggc
     2281 gtaatctatc tgggacagtg ctttccgtcc tcgttgtcat gctgaacgca gtgaagcatc
     2341 tcgtcttctt agctgcgaga cgagactctt cgctgcgctc agagtgacaa ggcatggacg
     2401 ggatatgaaa ggacgagctt gtgagtaatc ccttacgcgt aactgtctgg aacgagtacc
     2461 ggcacgagca gaatagcggc catccggcca gccgggtcta tccgcacggc atccatgagg
     2521 ccatcgccgc gtatttgcgc gagcagccgg ggctggaagt gcgcaccgcc acgctggacg
     2581 cgcccgacca gggcctgccc gccgaggcgc tggacactac cgacgtgctg atatggtggg
     2641 ggcatgacgc ccacgacgcg gtggccgatg cgctggtgga ccgcatccag gcgcgggtgc
     2701 tggcgggcat ggggctggtg gtgctgcact ccggccactt ctccaagatc ttcaagcggt
     2761 tgatgggcac cagttgcaat ctgcaatggc gcgagggcaa cgaccacgag cgcctctggg
     2821 tggtggagcc aggccattcc attgccgccg ggctgccgcc gtacatcgat ctggcgcacg
     2881 aggagatgta cggcgagcgc ttcgacatcc cggcacctga aacgctggtc ctggtaagct
     2941 ggttcagcgg cggcgaggtc ttccgcagcg ggtgttgcta cacgcgcgga ctgggcaaga
     3001 tcttctactt ccgccccggc cacgagagct tcccgaccta ctacgacccg ctggtgcgcc
     3061 aggtgatcgg caacgccgtg cgctgggccg cgcccgccgg gggtacgccg cctgtcttcg
     3121 gcaatgtgcc gccgcgcgag caacaatagc gccttagaaa gcgagtttct gccccatggc
     3181 taccagactc ccgccgcatc cgccgcggct gcccggcgtc ggcacgctgt ttccgtcgat
     3241 gcgcgacccc atcggctacc ccatccgcaa ctaccggcgc tacggcgagg tggtaggcgt
     3301 ccacctgttg aacgtgcatg gcgcggcgct ctacggcccg gcggcgaacc gctatatcct
     3361 ggtcgataac gtggagaact tcctggtcga gccgctggtg gaccggctgg gcgtacgctt
     3421 gctggtgggc gagggcgtgt tgtttatcga cgacccgcgc caccgcaagc agcgccgcct
     3481 gatcatgccc gctttccacc gcaaacggat cgagaattac caggaagtga tgcgccagga
     3541 gacgcaggcc atgctcgata gctggacgcc gggcgtggcg ttggacgtgg ccgccgagat
     3601 gcggcggctg gcgctggtca tcgccgggcg gacgctgttc agcatggacc tgggcggcaa
     3661 cgcgagcgaa ctgggggtgg ccgtcgctac gctggtgggg acactgggca gcgtgttcaa
     3721 cgcgaccctg gcccgcgtgc cgttcaacct gccgctgctg ggcgggcgcg gcggcagcgt
     3781 gcgggcgggg ctggcgcgca tcgaccgggt gctgggcgcg attatcgccg agcacgagcg
     3841 cgagggcacc gacaccggcg acgtggtctc gatgctggtg gcggcccgcg acgaggacgg
     3901 cagccggctc agtcccaagc agatccgcga ccacctgctg acgctgttcg tggccgggca
     3961 cgagacgagc gccaacgcgc tggcctggac cttctacctg ctggcccagc atcccgatgt
     4021 cacggcccgg ctgctagccg agttggaccg cgaactgcac ggcgccgcgc ccacgcccgc
     4081 cgacctggag cggctgccgt acctggagca ggtgatcaag gagtcgatgc ggctctaccc
     4141 gccggcgccc agcgcggtgc gcacggcgcg cgagggcttc gagtggcagg gctacaccat
     4201 tcgcgcgggc gaggtcgtct tctactcgcc ctatatcagc caccgcatgc cgcacatctt
     4261 cccggagccg gagacgttcc gcccggagcg cttcgcgccc gacagccccg agaacccgcc
     4321 gccgtatgcc tatatcccct tcgcggcggg gccgcgctcg tgcatcgggg cgcccttcgc
     4381 catgatggag atcaagacgg tgctggcgat gacgctgccg cgcttccgca tggacctggt
     4441 gcgcgggcag cgggtggacc aggcgatgcg cctgacgttg cagcccaaat acggtatcca
     4501 gatgaccccg cagccgcaag acggccaccc cgagcgctcg cgggcgcgcg tgctgggcaa
     4561 cgcggtcggg gcgacgccgg ggccgccctg agctactacc agtcatattt gtcactctga
     4621 gcgcagcgaa gagtctcgtc tcgatggatg cgcgacgaga ctcttcgctg cgctcagagt
     4681 gacaattgac gtgcgatatg ctacttcggc gcggggtcta gcgcctgggg cgcgtagcgg
     4741 cggatgaggg cgccgagatc gctggtctcc cagtcctgcg cgaagtagcc gaccgggatg
     4801 atgcggtcat agccggccat gctgccggag cgcggcaggc cccgcccgct cagcgcgccg
     4861 atggtggaca tgaccgggat ggcgccccgc ccaatccgca tccagccgtc catgtccagg
     4921 gccggttcgc gcagttgcag gccgcggatc aggccgcgcg tggtccagag ttcgcccgtg
     4981 ccctcgatgt tgtcccaggt ggtgcgcagg cggtagcccc aactgtagta ggcgatgccc
     5041 gccggaccgg cgacaaggcg gatgttgagg aaggccacgc agcaggcgat gccgagcgcg
     5101 gacaggatgg cggcgcctac aatgatcacg acctgggagg cggtatcgag cggctcgtcc
     5161 atgctcagcg gcagccagag catgaagccg gccaggccca ggcagagtag caggaagccc
     5221 ccggcgtaca ggtacatatg gcgtaggtga tagactctgg cggcctccgt ggccgtcgcc
     5281 tgattttcca cgatcatctt actcctcggg atgcgcttgt gcgtgctcac gccggttagc
     5341 acacaggctc atgttgcgaa gccacccggc gcgagtgggt tgacataatg gtgaggaacg
     5401 cacccggccc gtggcgggcg cggtgtccgt gtgttatatt gcggaccggt acggtagggt
     5461 acgcggtagt cggggcgacg ggccggtttc aaaccggccc ctacagaggc tgtgaggatc
     5521 agggggcggg tcggtttcaa accggcccct atagaagcgg gggaaaaggg cggcgggcag
     5581 aggcaggtga acctgcgcct gcccgccgcg gtatattgtt aggcggtgcc ggtggttcgc
     5641 gccggcgagc ggccctggcg ctattcggcg tcggggccga tgtaggcgcg aacctgagcg
     5701 gtttcgacct gccagacacg ctcggccatg tagggcttgt agcccaggtc gtagttgatc
     5761 ttgagcatgg ggccgttgga gtcggcgttg ccggtgcgca cgtagcggat ctgcgggcgc
     5821 tcacggatca ctttttccag catggcggcc ttgatccagc ggcccaggcc gcgattgcgg
     5881 tagggcggca gcacggtcgt ctcttcctgc cagagccgtt cggggtattg cgggtcccag
     5941 aacacttcgg tatagccggc gatggggccg gagtcgcctt cgcgcacgta catggtccag
     6001 cggataatgc cgcggcgggc gcggctggcg tcttgctcgc gcaactcttc gggcgtcgtg
     6061 atgtggtctt cccactcgat ggtgccgatg ggggccaggt tcatgccctt gcgcgcctcg
     6121 atcatggccg ggatcagttc ctccgggtgg gcaccttccc agaagcccag ttggaactcc
     6181 ggcgcgcgtt ccctggcccg ttcctgccag gcgcgcaact ggtcgcggtc cacgtcggcg
     6241 atgagcaact ggttggtgtg cgtctccagg cccatatggg cgccgaggcg attcaggaag
     6301 gcatcggttc caggtacccg gtcggtaccc caggtgacca gcaggcggcg ctgatcctgc
     6361 tcggcggcgg caacaacggg cgcgagcaag cgccgggcga tgccctggcg gcgcatatcc
     6421 gggcgcacgc cgatccagaa gtcggccatg tgcaggttgt cgttggggct ccagatggag
     6481 aacacaccgc gcccgacgat ctcgtcaccc tgccaggcgt gccaacggcg cacatcggca
     6541 taggccggga tgtcgcggac gtatttaata gtggtttcca gtttttcggg cggatcatcg
     6601 gggaaggctt cggcttccag gatgttgttc agcgcgttga gcgcccgcca ctccgcgccg
     6661 gtcgcggttt gcggatcgag gcggcggatc tcgacggcga gaatagtcat gatgcacctc
     6721 catggggcga tagacgggct gtgcgccgcc ggtgtgggcg cggtcgggtt
//
LOCUS       DASUYC010000731         2541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1727829, whole genome shotgun sequence.
ACCESSION   DASUYC010000731 DASUYC010000000
VERSION     DASUYC010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1727829"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="VF276_11980"
     CDS             complement(<1..723)
                     /locus_tag="VF276_11980"
                     /inference="COORDINATES: protein motif:HMM:NF013504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 6 protein"
                     /protein_id="HEX6607623.1"
                     /translation="MALVSALATALTAIAPLFAATGAAAASVNLDPNTRFYVPQPNHG
                     AIAQIAYLTSHQQQADANLIREMIAIPQAVWFTSGTPQGVQQSVKQIVQQAAGKGTVP
                     VLVAYNVPGRDCSQYSAGGAPTGDAYKAWIDGFAAGLGDHQAIVLLEPDGLALLPTDC
                     GQPDTYDRVSLINYAAHALLRDPNALVYLDAGHSAWHAVGDMAARLAAGGVLDVQGFF
                     LNVSNYQFTANLVQYGTWISSCI"
     gene            complement(1031..1822)
                     /locus_tag="VF276_11985"
     CDS             complement(1031..1822)
                     /locus_tag="VF276_11985"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX6607624.1"
                     /translation="MKHVTCREVELLLPATAAGALDVDEEETVRDHLAICPPCVKQLH
                     DFQDTVEQLAFAVPQVAPPVALRAKVLQAIAVTAPPAEGARPFPLAAAARPRQPRAPG
                     RFGAAYRRFAPAALAACLLLLVGSGIWIGIMTQRVEMMNDTLAQQQELRDLLQQPNSQ
                     MAMLKATNPDSAASGQAIMAPGHNRLGIMAAHLPTTGGTYQVWLLKHGNTAPVLAGHL
                     VCDGAGVAWAVVNTPADPNQMAGIRITDTEQGSSTGTPVLEGWYR"
     gene            complement(1985..>2541)
                     /locus_tag="VF276_11990"
     CDS             complement(1985..>2541)
                     /locus_tag="VF276_11990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6607625.1"
                     /translation="LATLYDQYDRLVFSFVFHILNDAASAEEVTQDVFLRAWEAAGKF
                     QVGRGRVSSWLLAIAHNRAIDELRSRRARGAKVTTSYDLATPGELEQAFRSSLGGATA
                     DEAVDPQEAALGGMRRVRIQEALRQLPPEQSRVIALAYYGGFTHREIAERLQEPLGTV
                     KTRLRLGLQKLRNLLAAEVQASEM"
BASE COUNT          396 a          804 c          898 g          443 t
ORIGIN      
        1 gatgcagctc gagatccagg ttccgtactg caccaggttg gccgtgaact ggtagttgga
       61 gacgttcagg aagaagcctt gcacgtcgag cacgccgccc gcggcgaggc gggccgccat
      121 gtcgcccacg gcgtgccagg cgctgtgccc ggcgtcgaga tagaccagcg cgttggggtc
      181 gcgcagcagg gcatgggccg cgtagttgat caggctgacc cggtcgtagg tatcgggctg
      241 gccgcaatcc gtgggcagca gcgcgaggcc gtcgggttcg agaaggacga tcgcctggtg
      301 atcgcccagg cccgcggcga acccgtcgat ccatgcttta taggcgtcgc ccgtgggcgc
      361 cccgcccgcc gagtactgcg aacagtcgcg accggggacg ttataggcga ccaggacggg
      421 gacggtgccc ttgcccgctg cctgctggac gatctgcttg acgctttgct ggacaccctg
      481 gggcgtgccg ctggtgaacc agaccgcctg cgggatggcg atcatttcgc ggatcaggtt
      541 ggcgtcggcc tgttgctgat gagaggtaag ataggcgatc tgcgcaatcg cgccgtggtt
      601 gggttgcggg acgtagaacc gggtgttcgg gtcgaggttc acacttgctg ccgcggcccc
      661 ggtcgcagcg aacagcgggg cgattgcggt cagcgcggtt gccagtgcgc taaccagggc
      721 caataaacgt ctgaaactcc tccggcgtgc catctgcgct cctttcgtcg atcccgctgc
      781 ccgccgggtg cccggcggcg gtgcggtctc gatcccctgg tgaatgtttc gccgctactc
      841 gcggcaggag ccaacaaccg gcacgctcct ggttttcgcg ccggccttac cgaagcgccc
      901 cctggtaggc atcttagcac cgaatcggcc tggagtcaaa gagcaatcgc cagccggcgc
      961 gggatgcaaa aaggggcgcc gccggggcgc ccctgtggtc atgatctatg tgtgctccgg
     1021 gcttcgcagg ctagcggtac cagccctcca gcaccggggt gccggtgctg ctgccttgct
     1081 cggtatcggt gatgcgaatc ccggccatct gattaggatc ggcgggcgta ttgacaaccg
     1141 cccaggccac tcccgccccg tcgcagacga gatgcccggc cagcaccggg gcggtgttgc
     1201 cgtgcttgag cagccagacc tggtaggtgc cgccggtggt cggcaggtgg gcggccatga
     1261 taccgagccg gttatggccg ggcgccatga tggcctggcc gctggccgcg ctgtcggggt
     1321 tggtggcctt cagcatcgcc atctgggagt tgggctgctg taggagatcg cgcagttcct
     1381 gttgctgggc cagggtgtcg ttcatcattt cgacccgctg ggtcatgatg ccgatccaga
     1441 tgccggagcc gaccagcaag agcaggcagg cggccagcgc ggcgggggcg aaccggcggt
     1501 aggcggcgcc gaagcggccc ggcgcgcgcg gttggcgggg gcgagccgcg gcagctaagg
     1561 gaaaggggcg ggcaccctcc gcgggcggcg ccgtaacggc gatggcctgc aataccttgg
     1621 cacgcaacgc gacgggcggg gctacctggg gcaccgcaaa cgccaattgc tcgaccgtgt
     1681 cctggaagtc gtgtagctgc ttcacacagg gcgggcaaat cgccaggtga tcgcgcaccg
     1741 tctcttcttc atcgacatcc agcgccccgg ctgccgtagc cgggagcagg agttctactt
     1801 ctcgacaggt aacgtgtttc atgatccttc cggcccgcac gagtcacaac tgctgggggg
     1861 tgacgtatag gttgaatgct cgctcgtccg atggcgccca gcacggccca tcggcgtctc
     1921 caccttttat acggaaaggc ccgagatttg gacgtacccg gcagccgcct tgccgccgag
     1981 ttggctacat ttcgctggct tgcacctcgg cggccagcaa attgcgcaat ttttgcagtc
     2041 ccagccgcag gcgcgtcttg acggtgccca gcggctcttg caggcgttcg gcgatctcgc
     2101 ggtgggtgaa gcccccataa taggccagcg cgatgacgcg gctttgctcg ggcgggagct
     2161 ggcgcagggc ttcctggatg cgcacccggc gcatgccgcc gagcgcggct tcctgggggt
     2221 cgacggcctc gtccgcggtc gccccgccca ggctggagcg gaaggcctgc tccagttcgc
     2281 cgggggtcgc caggtcgtag gacgtggtca ctttggcgcc gcgcgcccgg cggctgcgca
     2341 attcgtcgat ggcgcggtta tgggcaatcg ccagcagcca ggaggagacc cggccccggc
     2401 ccacctgaaa cttgcctgcg gcttcccagg cgcgcaggaa cacatcctgc gtcacctctt
     2461 cggcggaagc cgcatcgttg agaatgtgga agacaaagga aaagaccagc cggtcatact
     2521 gatcgtataa cgtggccagc g
//
LOCUS       DASUYC010000732         3019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1497786, whole genome shotgun sequence.
ACCESSION   DASUYC010000732 DASUYC010000000
VERSION     DASUYC010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1497786"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..943)
                     /locus_tag="VF276_11995"
     CDS             complement(80..943)
                     /locus_tag="VF276_11995"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6607626.1"
                     /translation="MIDLSPQLDDRVVRLDGLAVHVTARGPARAPLTFVGLNGLAGGA
                     DSFWPLLDGVPADVRVLLPDLPGCGGSEPLRERHTVEAYTGWLHRFLDEQGVDRVALI
                     SVATGAPVAIRYAGQHPARTAGLIFSLPFLGRIAVPKWMRPVAAYALGVRALRGLVDT
                     LRQNDALMHRIITHEPPEAIPEMAERDIDHKQQASLRAAGELLHDLMLMDSRTELRHL
                     HTPMLFLAAEHDAYSPLAVLQGIVRNHPERRLYIKWGAGHSWNEEFILAMQDEIRAFL
                     TRLQQQDGTPA"
     gene            complement(1385..1612)
                     /locus_tag="VF276_12000"
     CDS             complement(1385..1612)
                     /locus_tag="VF276_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607627.1"
                     /translation="MNPAHEEQPWTCPDCRGTGVRCPTCRGMRWLRTTFGPAGNRPLM
                     PCPDCTTPEQREATIARYIEAHEPAQDPPGQ"
     gene            complement(1647..2915)
                     /locus_tag="VF276_12005"
     CDS             complement(1647..2915)
                     /locus_tag="VF276_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607628.1"
                     /translation="MQPALHPATKAIVIDTPVLMLPALARLIGMDDAMLLQQIHYWLS
                     LGGHEHDGRRWIYNTYHAWAEQFPGWSKDKVRRIVERLRGRGLVQTANYNRHAYDQTT
                     WYTIDYAAVAALGIPMAPLPGALAGFSPSTLADGPAPAIVPALADAPAPAPAAGPAQR
                     PPALLPASTEAPAPAPVPAVSAGVPGTPGIVCAPAVPEACPAERSAEARRAPATSLGA
                     DAPRYSAPANRGGADASRCGADASRCGADASRCGASARRCCDFAPTIPETTTETTSEI
                     TSEINTRERERDGTPSPSAFEHFTTTETLTGWLAAECAMMGVAPTGVNAAHETDKWRD
                     EIRAGRRAVPADAAADWRQWMRRALAYTRRHPPQGAPTHGPVSLATRSAQADSGELAA
                     REIAAAARLQELVLGRMSQAAAGSAGAAAL"
BASE COUNT          438 a          952 c         1134 g          495 t
ORIGIN      
        1 tgaacgaagg acgcgaagat catttatatt aatcttattc ttcgcgctct tcgtgccttc
       61 gcggtgaact atccgtcgct catgccggcg tgccgtcctg ctgctgcaag cgcgtcagga
      121 aggcgcggat ttcgtcttgc atggcgagga tgaactcctc gttccaggag tggcccgcgc
      181 cccacttgat gtagagccgg cgctcggggt ggttgcggac gatgccttgc agcaccgcga
      241 gcggcgagta ggcgtcgtgc tcggcggcca ggaagagcat tggcgtgtgc aggtggcgga
      301 gttcggtgcg gctgtccatc agcatcaggt cgtgcagcag ttcgcccgcg gcgcgcagcg
      361 aggcttgctg cttgtggtcg atgtcgcgct cggccatttc ggggatggcc tccggcggct
      421 cgtgggtgat gatgcggtgc atcagggcgt cgttctggcg cagggtatcg accaggccgc
      481 gcaacgcccg cacgcccagc gcgtaggcgg ccaccgggcg catccacttg ggcacggcga
      541 tgcggcccag gaacggcagg ctgaagatga gccccgccgt gcgcgccggg tgctgcccgg
      601 cgtagcggat cgccaccggc gcgcccgtcg ccacgctgat cagcgcgacg cggtccacgc
      661 cctgctcgtc gaggaagcgg tgcagccagc cggtgtacgc ctccacggtg tggcgctcgc
      721 gcaggggttc gctgccgccg cagccgggca ggtcgggcag cagcacgcgc acgtcggcgg
      781 gcacgccgtc gagcagcggc cagaagctgt cggcgccgcc ggccagcccg ttgaggccca
      841 cgaaggtcag cggggcgcgc gccggccccc gtgccgtgac atgcaccgcc agcccatcga
      901 ggcggaccac gcggtcgtcg agttgcggac ttaaatcgat cacaggcgct ctcccgcccg
      961 gaggctcaag cctccggcta ccgttacgaa ggccgcctgc gcggcctgtt ccctctcccc
     1021 tagcccgcgc aggcgggctt cgtaaggcta gcccgcccgt tcacgggccg ggcgtcgccg
     1081 catcggccat ttataacggc gcggggccgt ccgctgtaac tcgcgggcgg gggcgtcgtc
     1141 ttttcaccgc gaaggaacga aggcaacgaa ggacgatgga gtgcaccgca gagacgccga
     1201 gaacgcagag aacgattcag ggtttgatta cgacggagtg caccgcgacg gcgcgaaggc
     1261 cgcagaggat ttaggggttt cactcctagc gaataactct tacaatcctt cgcgttcttc
     1321 gcgccttcgc ggtgaatatc cataaaaacc tcggcgttct ctgcgtctct gcggtgcaat
     1381 gcgtctactg ccccggcggg tcctgcgcgg gctcgtgcgc ctcgatgtag cgggcgatcg
     1441 tcgcctcgcg ctgctcgggc gtggtgcagt cggggcacgg catcaacggc cgattgccgg
     1501 ctgggccgaa cgtggtgcgc agccagcgca tcccgcgaca ggtgggacag cgcacgcccg
     1561 tgccgcggca gtcggggcag gtccagggct gctcctcgtg cgcggggttc atgctgccgc
     1621 cctgttcgcc ggtgccttta cacggctcac agcgcagccg ctcccgcgct tccagcggca
     1681 gcttgggaca tacggccgag aacaagttct tggagccggg cggcggcggc gatttcgcgg
     1741 gcggcgagtt cgccagaatc cgcttgagct gatcgtgtag cgagtgaaac gggtccatgc
     1801 gtgggggctc cttggggtgg gtggcggcgc gtgtaggcca gggcgcggcg catccactgc
     1861 cgccaatcgg cggcggcgtc ggcgggcacg gcccggcgcc cggcgcggat ctcgtcgcgc
     1921 catttgtcgg tctcgtgggc ggcattgacc ccggtggggg cgacgcccat catggcgcac
     1981 tcggcggcca accagccggt gagcgtctcc gtcgtcgtga aatgttcaaa cgcagagggg
     2041 gagggggtgc cgtctctctc tctctctctg gtgttaatct ctgacgtaat ctctgacgta
     2101 gtctctgtag tagtctctgg tatcgtcggc gcaaaatcac aacatcgtct cgcactggcg
     2161 ccgcatcggg acgcatcagc gccgcatcgg gacgcatcag caccgcatcg ggacgcatca
     2221 gcgccgcctc ggttggcagg ggcgctatat cgaggggcat ccgcgccaag ggaggtggcg
     2281 ggggcgcgcc gagcctctgc gctgcgctca gcgggacaag cttcgggcac ggcgggggcg
     2341 cagacgatgc cgggggtgcc ggggacgcct gcggagacgg cggggaccgg cgctggggcc
     2401 ggtgcctcgg tggaggcagg cagcagcgca ggggggcgct gggcggggcc ggcggcaggc
     2461 gccggcgcgg gggcatcggc cagggcgggc acgatggccg gggcggggcc gtccgccagg
     2521 gtggacgggg agaacccggc cagggcgccg gggagcgggg ccatcgggat gccgagggcg
     2581 gcgacagcgg cgtagtcgat ggtgtaccag gtggtctggt cgtaggcgtg gcggttgtag
     2641 ttcgccgtct gcaccagccc gcgcccgcgc agccgctcga cgatgcggcg cactttatct
     2701 ttgctccagc cggggaactg ctcggcccag gcgtggtagg tgttgtagat ccagcgccgg
     2761 ccgtcgtgct cgtggccgcc caggctgagc caatagtgga tctgctgcaa gagcatggcg
     2821 tcgtccatgc cgatgaggcg ggccagggcg ggcagcatga gtacgggggt gtcgataacg
     2881 attgcttttg tggcagggtg aagcgcgggc tgcatacagg agcctcccat ggtgcgtggt
     2941 attcgagcaa atccgactgg agtcagggcc aacggagccg ctgggccggg gacggcgccc
     3001 aggggcctat ctgcaccta
//
LOCUS       DASUYC010000733         2018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1950086, whole genome shotgun sequence.
ACCESSION   DASUYC010000733 DASUYC010000000
VERSION     DASUYC010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1950086"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VF276_12010"
     CDS             complement(<1..486)
                     /locus_tag="VF276_12010"
                     /inference="COORDINATES: protein motif:HMM:NF014177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEX6607629.1"
                     /translation="MRYIGSKDRLLPFIQQALDDHGIQGGTFCDAFSGTTVVARHAKT
                     RGYRVIGNDLLFFSYVLQRAYLCCNDYPRFAGLPWAAETPGPDGPLCAVIHHLNALPP
                     QPGFIYQNYSPGEGDAPGRRRYLSASNAGRIDAIREQIAAWWAGGQLAGEEPYLLLAA
                     LI"
     gene            669..971
                     /locus_tag="VF276_12015"
     CDS             669..971
                     /locus_tag="VF276_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX6607630.1"
                     /translation="MTSSATQPAATETRPQMTVTARFFALYREAAGQDQLVVTVPEGT
                     TVGGLWQYLRDAAPGLASAEAATAYTAYAVNGAWAKNSRVLAGGDEVAFLPPVSGG"
     gene            977..1426
                     /locus_tag="VF276_12020"
     CDS             977..1426
                     /locus_tag="VF276_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEX6607631.1"
                     /translation="MSGATGAGPALGPFEITAAPLSVDQLAAEVQTPADGAVVTFVGL
                     VRDNHQGKRVVALEYEAYPELAERELRALSEDIAAKYGLHGIGIRHRTGRLAIGEISV
                     VIAVAAPHRAEAFAACAEALDRLKATVPVWKKEWYEDGSTWLGQAAG"
     gene            1482..>2018
                     /locus_tag="VF276_12025"
     CDS             1482..>2018
                     /locus_tag="VF276_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010400833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX6607632.1"
                     /translation="MGQYIADTLDRPLRDLRISVTDRCNFRCVYCMPKEVFGRDFQFL
                     PSAQLLTFEEITRLTRIFVDHGVEKIRLTGGEPLVRRDLEKLIAMLSQIEGVRDLTLT
                     TNGSLLVKKARALKDAGLARITVSLDSLDDAVFKAMNDVDFPVGRVLDGIAAARAAGL
                     WPIKVNMVVKRGVNEHSVV"
BASE COUNT          351 a          670 c          701 g          296 t
ORIGIN      
        1 gatcagcgcg gccagcagca ggtacggttc ctcgcccgcc agttgcccgc cggcccacca
       61 cgccgcgatc tgctcccgga tggcgtcgat gcgcccggca ttggacgcgc tcagatagcg
      121 ccgccggccc ggcgcgtccc cctcgccggg gctgtagttt tggtagataa aaccgggctg
      181 cggcggcagg gcgttcaaat ggtggatgac cgcgcacaac ggcccgtcgg ggccgggcgt
      241 ctccgccgcc cacggtaggc ccgcgaagcg cgggtagtcg ttgcagcaaa ggtaggcccg
      301 ctgcaagacg taggaaaaaa acagcaggtc attgccgatc acccggtagc cgcgcgtttt
      361 cgcgtggcgg gcgaccacgg tcgtgccgct gaaagcgtcg caaaaggtgc cgccctggat
      421 gccgtggtcg tccagggcct gctggataaa cggcaacagc cggtctttgg agccgatgta
      481 gcgcacgggc ctattatagc atgccggcga atcacccggc agccgggatc agccggacgc
      541 ggcggcaaat tctggtgaat ttaggtgaaa accggcaaaa tcggttgacg cggcgggctg
      601 gctgtggtaa catgcgagat aggcatggcc cgaccggccc gccggcccag gaaatagaaa
      661 gaatcgctat gaccagcagt gcaacccaac ccgccgcgac ggagacccgt ccgcaaatga
      721 ccgtaaccgc gcgcttcttc gcgctctacc gcgaggccgc cgggcaagac caactggtgg
      781 tcacggtgcc ggagggcacc accgtcggcg gcctctggca atatctgcgg gatgccgcgc
      841 cgggcctggc ctcggcggaa gcggcgacgg cctataccgc gtatgccgtc aacggcgcct
      901 gggccaaaaa cagccgggtg ctggccggcg gcgacgaggt ggcgttcctg ccgcccgtca
      961 gcggcgggtg agccgggtga gcggcgccac gggggccggg cccgcgctgg gccccttcga
     1021 gatcaccgcc gccccgctgt cggtcgatca actggcggcg gaggtgcaga cgcccgccga
     1081 cggcgcggtg gtcaccttcg tcgggctggt gcgcgataac caccagggca agcgcgtggt
     1141 cgcgctggag tacgaagcgt accccgaact ggccgagcgc gagctgcggg cgttgagcga
     1201 agacatcgcc gccaagtacg ggctgcacgg catcggcatc cgccaccgca cggggcggct
     1261 ggcgattggc gagatcagcg tggtcatcgc ggtggccgcg ccccatcgcg ccgaagcctt
     1321 cgccgcctgc gccgaggccc tcgaccggct caaggccacc gtgccggtct ggaaaaaaga
     1381 atggtacgaa gacggaagca cctggctggg gcaggccgcc ggctaaaccg gcgcggatcg
     1441 ccaggcgctt cgtgggacac gctcagaacg gacagctttt gatgggtcag tacatcgccg
     1501 atacacttga ccggcctctc cgggatctac gcatctcggt gacggaccgc tgcaacttcc
     1561 gctgcgtcta ctgcatgccg aaggaagtgt ttggccgcga tttccagttc ctcccctccg
     1621 cccaactgct caccttcgag gaaatcaccc ggctcacgcg catcttcgtc gaccacggtg
     1681 tggagaagat ccgcctgacc ggcggcgaac cgctggtgcg ccgcgacctg gaaaagctga
     1741 tcgccatgct gtcgcagatc gagggcgtgc gcgacctcac cctgaccacc aacggctcgc
     1801 tgctggtcaa gaaggcccgg gcgctcaagg acgccgggct ggcgcgcatc acggtcagcc
     1861 tcgactcgct ggacgacgcc gtgttcaagg ccatgaacga cgtggacttc ccggtgggcc
     1921 gcgtgctcga cggcatcgcg gcggcgcggg ccgccgggct gtggccgatc aaggtcaaca
     1981 tggtggtgaa gcgcggcgtg aacgagcact ctgtggtc
//
LOCUS       DASUYC010000734         3056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_70169,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000734 DASUYC010000000
VERSION     DASUYC010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70169"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="VF276_12030"
     CDS             complement(<1..1045)
                     /locus_tag="VF276_12030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX6607633.1"
                     /translation="MIDPADSPAPGIVIYDARGLVVFSNPAAQALLGPAAAPGRADSD
                     PAQPPLLYADGTPCPPEDRPAVRARRTGQAVRQTVLGVPGASDQPATWWLVDAEPLRA
                     AGGPVTQIVVSFVDITARSGAQQSRPWGEQEFLALVENAPDVIARYDRSLRHVYVNRA
                     VEQATGWPADIWIGKSLRELDIPADQIAFWEARLAAVFADGQEQVVEYQGYSPAEPRW
                     YESRLTPEFAPDGSVEYVLNIARDITDRHVARQALQESEARFRLMFAGNPLPMWLYDM
                     QTLAFLEVNDAAITNYGYSRAEFLAMRITDIRPAEDVPLLLDDVARTRPSYQQSGYWR
                     HRRKDGTILEVQVT"
     gene            complement(1301..>3056)
                     /locus_tag="VF276_12035"
     CDS             complement(1301..>3056)
                     /locus_tag="VF276_12035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6607634.1"
                     /translation="TRAEAVGGSWRALHLWADPQQPEQLAGAMSRAGRVRDIEFDFHP
                     RSGELRRGLLSAELLEIDGVPHVLSIVRDITESKQAADAFQRQYDFTTAITGSLGEGV
                     YALDLEGRVTFANPAATAMLGWTEAELRGRPMHDLIHFQRADGTRFPASECPLHGVAV
                     PGDRIVVDDDVYTRKDGSIFPIAYTSAAITTGGQVIGQVVAFRDITERNLMESRARAA
                     ERLGALGRVAAGVAHEFNNILAGMMGRLDLLALTVHEPEAVEILRLLQQTVDDGVAMV
                     SRIQAFARLRDAGEMTAVPVSDLVNDVVSLTHPRWHARPAVAGGSITLRTQVDEGLLL
                     QGNPTKLREVLTNLIFNAVDALPHGGLIQITAEARAGTVALVVRDTGAGMDAATQARI
                     FEPFFTTKVTGSGLGLAVVKDIIESHGGAIRVVSQLGGGTVFTITLPAAHAPGVPPAP
                     PAPPPRQGRARILAVDDDPGLADMLRAMLAVGGHTVTVATSGAEAVRMFEQQPFDVVC
                     TDLGMPGMNGWEVARHVRARAPQTPVILITGWGAALDPDELAAGTVDFVLPKPYRVVQ
                     VQEIVAQALARRAAGDSG"
BASE COUNT          432 a         1077 c         1104 g          443 t
ORIGIN      
        1 aggtgacctg cacttcgagg atggtgccgt ccttgcgccg gtggcgccag tagccggact
       61 gctgatagct gggccgcgtc cgcgccacgt cgtcgagcag caacggcacg tcctccgcgg
      121 ggcgaatatc ggtgatgcgc atcgccagaa actcggcgcg gctgtagccg tagttcgtga
      181 tcgccgcgtc gttcacttcg aggaaggcga gcgtctgcat atcgtagagc cacatcggca
      241 ggggattgcc ggcgaacatc aggcgaaagc gggcttcgct ctcctgcaac gcctgccggg
      301 cgacatgccg gtcggtgatg tcgcgggcga tgttgagcac atattcgacc gagccgtcgg
      361 gcgcgaactc gggcgtgagg cgcgactcat accagcgcgg ctcggccggc gaatagccct
      421 ggtattcgac cacctgctcc tgcccgtcgg cgaacacggc ggccagcctg gcctcccaga
      481 aggcaatctg atcggcgggg atgtccagct cgcgcaggct cttgccgatc cagatgtcgg
      541 cgggccaacc cgtggcctgc tccacggcgc ggttgacgta gacgtggcgc aggctccggt
      601 cataccgggc gatcacgtcg ggcgcatttt ccaccagggc gaggaactcc tgctcgcccc
      661 aggggcgaga ctgttgcgcg ccggagcgcg cggtaatatc gacgaaagag acgacgatct
      721 gcgtcaccgg cccgcccgcc gcgcgcagcg gctcggcgtc gaccagccac caggtcgcgg
      781 gctggtccga ggcgccgggc acgcccagca cggtctggcg caccgcctgg ccggtgcggc
      841 gggcgcgcac cgcgggccgg tcctccggcg ggcagggcgt gccgtccgcg tagagcagcg
      901 gcggctgggc cgggtcgctg tcggcccggc ccggcgcggc cgccggcccc agtagcgcct
      961 gggcggcggg attgctgaac accacgaggc cgcgggcatc gtagatcacg atgccgggcg
     1021 ccggagagtc tgcgggatcg atcatagcgc gcgcctttgc cggggaacgg tgcgcgccgg
     1081 cgggtccgcg cgccgggcgg cccaacgtag acacgggggc gacgggtgcc tctttagatg
     1141 cccctttagg tgccccttta ggtgcccctt taggggcagg cgcggttgca cgcgtgcgct
     1201 acctttcctg ggattctagc acggcccgct ccccggccac aaggggcaga caggccaccg
     1261 ggccgggtcc gatagtcccc aatccgcgcc gggccacgcc ttagccggag tcgccggccg
     1321 cgcggcgggc cagcgcctgg gccacgatct cctgcacctg caccacgcgg tagggtttgg
     1381 gcaggacaaa gtccaccgtc ccggcggcca gttcatcggg atcgagcgcc gcgccccagc
     1441 cggtgatgag gatgaccggc gtctgcgggg cgcgagcgcg cacgtggcgg gccacttccc
     1501 agccgttcat gcccggcatg cccagatcgg tgcagaccac gtcgaacggt tgctgctcga
     1561 acatgcgcac cgcctcggcg ccgctggtcg ccacggtcac ggtatggccg ccgacggcca
     1621 gcatggcgcg cagcatatcg gccaggccgg ggtcgtcgtc cacggccagg atgcgggcgc
     1681 ggccctggcg gggcggcggg gcgggcggcg cgggtgggac accgggcgca tgggcggcgg
     1741 gcagcgtgat agtaaacacc gtgccgccgc ccagttgcga caccacccgg atcgcgccgc
     1801 cgtggctctc gatgatgtcc ttgacgacgg ccaggcccag gccgctgccc gtgaccttgg
     1861 tggtgaagaa tggctcgaag atgcgcgcct gggtcgccgc atccatgccc gcaccggtat
     1921 cgcgcaccac tagcgccacg gtgccggcgc gcgcttccgc cgtgatctgg atcagcccgc
     1981 cgtgcggcag cgcatcgacg gcattgaaga tgaggttggt cagcacctcg cgcagcttgg
     2041 tcgggttgcc ctgcagcagc aggccctcgt ccacctgggt gcgcagggtg atgctgccgc
     2101 ccgccaccgc cgggcgcgcg tgccagcgcg ggtgggtcag gctgaccacg tcgttgacca
     2161 ggtcgctcac cggcaccgcg gtcatctcgc cggcatcacg caggcgggcg aacgcctgga
     2221 tgcggctgac catcgccacg ccgtcgtcca ccgtctgctg caagaggcgc aagatctcca
     2281 ccgcctcggg ttcgtgcacg gtcagcgcca gcaggtcgag gcggcccatc atgccggcca
     2341 ggatattatt gaactcgtgg gccacgcccg cggccacccg gcccagcgcg cccaaccgct
     2401 cggcggcgcg cgcccggctc tccatcaggt tgcgctcggt gatgtcgcgg aaggcgacca
     2461 cctggccgat cacctggccc cccgtggtga tcgccgccga cgtgtaggcg atcgggaaga
     2521 tgctgccgtc cttgcgcgtg tacacatcgt cgtccacgac aatgcggtcg ccgggcacgg
     2581 ccacgccgtg caaggggcac tcgctcgcgg ggaagcgtgt gccgtcggcg cgctggaagt
     2641 ggatgaggtc gtgcatgggc cggccccgca actcggcctc ggtccagccc agcatcgccg
     2701 ttgccgcggg gttggcgaag gtcacgcggc cctccaggtc cagcgcgtag acgccttcgc
     2761 ccaggctgcc ggtgatggcc gtggtgaagt cgtactggcg ctggaaggcg tcggcggcct
     2821 gcttgctctc ggtgatgtcg cggacgatgc tcaacacatg gggcacaccg tcgatctcca
     2881 gcagctccgc cgagagcagg ccccggcgga gctcgcccga ccggggatgg aagtcgaact
     2941 cgatgtcgcg gacgcgcccg gcccggctca tcgccccggc caactgctcc ggctgctgcg
     3001 ggtcggccca caaatgcagg gcgcgccagg acccccccac cgcctcggcg cgcgtg
//
LOCUS       DASUYC010000735         4362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1959764, whole genome shotgun sequence.
ACCESSION   DASUYC010000735 DASUYC010000000
VERSION     DASUYC010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4362
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1959764"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="VF276_12040"
     CDS             complement(<1..762)
                     /locus_tag="VF276_12040"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HEX6607635.1"
                     /translation="MALPSPAAPAPSGAPPAAPAGGQFGLPDLRIVPLADVIPHEQAD
                     PRRVERLSRRIAGDHLLKNPIIVTPIPGSNQFMVMDGANRTAALGQVGARDVLAQIVR
                     YDQPGVQLTTWNHLITGIEPNTLLYAIGAIPGLELQETPLPAARAALEARDAVAYLVH
                     PPHGTHEVEVYVLKAVHLDPQAETALLLEMVDLYKGKPETAIHRVGSDDIAAYLDDYD
                     DVSALIVFPPYQPAEILELARAGLRVPTGITRHIIP"
     gene            complement(763..1167)
                     /locus_tag="VF276_12045"
     CDS             complement(763..1167)
                     /locus_tag="VF276_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012187967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEX6607636.1"
                     /translation="MDSLYREVILEHYRQPHHKGHLAEPTVSVEVDNPLCGDRLAIDL
                     ALQDGVITDVAFTGRGCAISQASASILSDELVGKPVAEAAELTWHDMMDLLGVEVGPA
                     RLKCALLALRAVKMALTEAGVPVTSYVDEDEA"
     gene            complement(1248..1886)
                     /locus_tag="VF276_12050"
     CDS             complement(1248..1886)
                     /locus_tag="VF276_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6607637.1"
                     /translation="MIRLLICEDQTLMRQGLATILGLEPGLTVVGEAADGAEAIAQVR
                     ALHPDIVLMDVQMPGMDGIEATRLITAAYPDTRVIILTTFDYEEYVFEGVKAGAMGYM
                     LKDSPAPDLIHTIRRVHAGERFIQPAVASKLLFEFAQGARRGSGADYEPLSERETDIV
                     RRLAQGMSNREVADDLALAEGTVKNYVSTILGKLHAANRVQAINLARQYKLI"
     gene            complement(1883..3271)
                     /locus_tag="VF276_12055"
     CDS             complement(1883..3271)
                     /locus_tag="VF276_12055"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX6607638.1"
                     /translation="MPDDQTKQEAPLPAQWKMPAVIDSFLVLYYFVTWIAIGGYTAAA
                     FADAPGRAAGFLLPAALYGALLLSFPVLNWRRYRRERSAINDLLWGYDEQRIEQIVYV
                     LVDFGLIGWLSALHPTFTAMYFLLYGHTWGILHWNGRLAFAVVAAEIVAHLYQLGLIT
                     LFLTEKTLPAEFWIYTFAIASCLVYGGMIMRLIRSRIESEILVHELRQTKQQLEEALA
                     KEKEVAVLRERNRMAREMHDVLGHALSLVAVKIEAAQRLQAVDPARAAAELEATKTLV
                     RESMTDLRASLAQLRSPELEASDRALDEALREWVTRTGREAGIEVECIFEPAVAAVPP
                     PIQDALWRVGREAVLNTVKHARARRIEIHLFRKDNMVYLSVADDGVGIPHLAEGTARL
                     EGAGHYGVRGMRERLEALGGRLTMRPNPDGHGTLVLAGVPLPPAEPPPPTRLRDRLSP
                     ARRLLFRKGEHS"
     gene            complement(3412..4200)
                     /locus_tag="VF276_12060"
     CDS             complement(3412..4200)
                     /locus_tag="VF276_12060"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6607639.1"
                     /translation="MLTDATTVATPSPVQIAGRRFGLLLQTNLLLYLRNRMAVFWNMV
                     FPIGLMLLFGTIYGREPAVITYLATGMVVLSLLSNGLIGNAATMALWRERGILRRIQT
                     TPLPLWQLLLARIVTQGAIMILQACLLIGTSVLVFGAQFEISNLIAAVPAVVLGALLF
                     MAFGQALAALVQKVDTVNIIGQVIYFPLMFLGNIFLPLQQLPDALQTVGKWFPSALIA
                     DLVRTTMLGQRTADMILPLPVTLLGVACYFVAAVVIAVRFFKWR"
BASE COUNT          713 a         1456 c         1463 g          730 t
ORIGIN      
        1 cggaataata tggcgcgtga tcccggtcgg gacgcgcaac ccggcccgcg ccagttccag
       61 gatctccgcc ggctggtatg gcggaaagac gatcagcgcg ctcacgtcgt cgtagtcgtc
      121 cagatacgcg gcgatgtcgt cgctgcccac ccggtggatc gccgtctccg gcttgccttt
      181 gtagagatcg accatctcca gcaggagggc cgtctccgcc tgcgggtcga gatgcacggc
      241 tttgagcacg taaacctcga cctcatgcgt gccgtggggc ggatggacca gataggccac
      301 ggcgtcgcgc gcctctagcg ccgcgcgggc ggcgggcaac ggcgtttcct ggagctccaa
      361 ccccggtatc gcgccgatcg catagagcag cgtgttcggc tcgatgccgg tgatcaggtg
      421 attccaggtc gtcaattgga cgcccggctg atcgtagcgc acgatctggg ccagcacatc
      481 gcgggcgccg acctgcccca gcgcggcggt gcggttggcg ccgtccatga ccatgaactg
      541 gttgctgccg gggatgggcg tcacgatgat cgggttcttg agcaggtgat cgccggcgat
      601 gcggcggctc aggcgctcga cccggcgcgg gtccgcctgc tcatgcggga tcacatcggc
      661 cagcggcaca atgcgcagat ccggcagacc gaattgcccg ccggcgggcg cggcaggggg
      721 cgcgccggat ggcgccggcg cggcgggcga gggtagcgcc atctacgcct cgtcctcgtc
      781 cacgtaggac gtgacgggca ccccggcctc ggtcagagcc atcttcacag cccgtagcgc
      841 cagcagcgcg cacttcaagc gcgccggccc cacctcgacc cccagcaggt ccatcatgtc
      901 gtgccaggtg agttccgcgg cttcggccac cggcttgccg accagctcgt cgctcaggat
      961 cgaggccgac gcctggctga tggcacagcc gcgcccggtg aaggccacgt cggtaatcac
     1021 gccgtcctgc agcgccaggt cgattgccag ccggtcgccg cacagcgggt tatccacctc
     1081 gaccgacacg gtcggctcgg ctaaatgccc cttgtgatgc ggttggcgat aatgttcgag
     1141 gatgacctcg cgatacaacg aatccatact actattgtac ccgattccgg cgcgacactc
     1201 aaccaggcaa gcccggcgaa atgcgccttc tagtccggcg cgacccgtca gatcagctta
     1261 tactgccgcg ccaggttgat cgcctgtacg cggttggcgg cgtgcagttt gcccaggatg
     1321 gtggacacat agttcttgac ggtgccctcg gccagggcca ggtcgtcggc cacctcgcgg
     1381 ttgctcatgc cctgggccag ccggcggacg atgtcggtct cgcgctcgct cagcggttcg
     1441 taatcggcgc cgctgccgcg ccgggcgccc tgcgcgaact cgaacagcaa cttgctcgcc
     1501 acggcaggct ggatgaaccg ctcgcccgcg tggacgcggc ggatggtgtg gatcagatcg
     1561 ggcgccgggc tgtccttgag catatagccc atggcgcccg ccttcacgcc ctcgaacaca
     1621 tactcctcgt agtcgaacgt ggtcaggata atgacccgtg tgtccgggta ggcggcggtg
     1681 atcaagcgcg tggcctcgat gccgtccatg cccggcatct gcacgtccat cagcacgata
     1741 tcggggtgca gcgcgcgcac ctgcgcgatg gcttcggcgc cgtccgccgc ctcgcccacc
     1801 acggtcaggc ccggctccag gcccaggatc gtcgccaggc cctggcgcat cagtgtctgg
     1861 tcttcacaga tcagtaatcg gatcacgagt gttccccttt gcggaacaag agccggcgcg
     1921 cgggcgagag ccggtcgcgc aggcgggtgg ggggcggcgg ttcggcgggc ggcagcggca
     1981 ccccggccag caccagggtg ccgtggccgt cgggattggg gcgcatcgtg agccgcccgc
     2041 ccagcgcctc cagccgttcg cgcatgccgc gcacgccgta atggcccgcg ccttcaagcc
     2101 gcgccgtgcc ctcggccagg tgcgggatac ccacgccgtc gtcggctacc gagaggtaga
     2161 ccatgttgtc tttgcggaac aggtggatct cgatccggcg ggcgcgggca tgcttgacgg
     2221 tgttcaagac ggcttcgcgg cccacgcgcc agagcgcgtc ctggatcggc gggggcaccg
     2281 cggcgacagc gggctcgaat atacactcga cctcgatgcc cgcttcccgc ccggtgcgcg
     2341 tgacccactc gcgcagcgcc tcgtccagcg cccggtcgct cgcctccaac tcggggctgc
     2401 gcagttgcgc cagcgaggcc cgcagatcgg tcatgctctc gcgcaccagc gtcttggtcg
     2461 cttcgagttc ggcggcggcg cgggccgggt ccaccgcttg caggcgttgg gccgcctcga
     2521 tcttcacggc caccagggac agcgcgtggc ccagcacgtc gtgcatctcg cgcgccatgc
     2581 ggttgcgctc gcgcagtacg gccacctctt tttccttcgc cagtgcctct tcaagctgct
     2641 gcttggtctg ccgcagttca tggaccagga tttcgctctc gatgcgcgac cggatcagcc
     2701 gcatgatcat gccgccgtag accaggcaac tggcgatggc aaaggtatag atccagaatt
     2761 ccgccggcaa ggtcttctcg gtgaggaaca gcgtgatcag cccgagttgg tataggtggg
     2821 cgacgatttc cgccgcgacc accgcgaagg ccagccgccc gttccaatgt agtatgcccc
     2881 aggtgtgccc gtagagcagg aagtacatcg ccgtgaacgt cgggtggagc gcgctcagcc
     2941 agccgatcag cccgaaatcg accaggacat atacgatctg ctcgatccgt tgctcgtcgt
     3001 agccccagag caggtcgttg atcgcgctgc gctcgcgccg gtagcggcgc cagttcagca
     3061 cgggaaagct cagaagcagc gcgccgtaca gggccgcggg cagcagaaac ccggcggccc
     3121 gccccggcgc atcggcaaag gcggcggcgg tatagccgcc gatggcgatc caggtcacga
     3181 agtagtacaa aaccaggaag ctgtcgatga cggccggcat cttccactgc gcgggcagcg
     3241 gggcctcctg ttttgtctga tcgtcgggca tggccgtttg tcctttgctg catcatcact
     3301 gtaacaccgg catccattat agcacgccgg tcgagtcgca ctgcatcccg gctcttgcgc
     3361 cgtctccccc ttcccggcgc cgggaagggg gcaggggggt taggcccgtc gtcaacgcca
     3421 cttgaagaac cgcaccgcga tcaccaccgc ggcgacgaag tagcaggcaa cgcccagcag
     3481 ggtcacgggc agcggcagga tcatgtcggc agtgcgctgg ccgagcatcg tggtgcgcac
     3541 caggtcggcg atcaaggcgc tggggaacca cttgcccacc gtttgtaacg catcgggtag
     3601 ttgctgcagc gggagaaaga tgttgcccag gaacatgagc gggaagtaga tgacctgccc
     3661 gatgatgttg accgtgtcca ccttctggac gagcgccgcc agcgcctggc cgaaggccat
     3721 gaacagcagc gcgcccagca ccaccgccgg gacagcggcg atcagattgc tgatctcgaa
     3781 ctgcgcgccg aacacgagca cgctggtgcc gatgagcagg catgcctgga ggatcatgat
     3841 cgcgccctgg gtgacgatgc gcgccagcag caactgccac agcggcagcg gcgtcgtctg
     3901 gatgcggcgc aggatgccgc gctcgcgcca gagcgccatg gtggcggcgt tgccgatcag
     3961 gccgttcgac agcaggctga gcacgaccat gccggttgcc aggtaggtga ttaccgccgg
     4021 ttcgcgcccg tagatcgtgc cgaacagcag catcagcccg atgggaaaga ccatgttcca
     4081 gaacacggcc atgcggttgc gcaggtagag cagcaagttg gtctgcaaga gcaagccgaa
     4141 ccggcgcccg gcgatctgga ccggggatgg cgttgcgacg gtggttgcat cggttagcat
     4201 ttcaattact ccttattcgc gatgtgccgg gccggtttgc aaccggcccc tacagcgtta
     4261 ccagctgccg ggccggtttg caaccggccc ctacagcgtt accagttgcc gggccggttt
     4321 gcaaccgacc cctgcggggt tggctggtgg ccggccggct tg
//
LOCUS       DASUYC010000736         2420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1728473, whole genome shotgun sequence.
ACCESSION   DASUYC010000736 DASUYC010000000
VERSION     DASUYC010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1728473"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..976
                     /locus_tag="VF276_12065"
     CDS             242..976
                     /locus_tag="VF276_12065"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6607640.1"
                     /translation="MPATPREQRIRTTLLRTAFALLYTRFAWAYDWVSRTFFHGEWRR
                     WQRAALEPLAGLPGGRVLEVGFGTGDLQADLRAAGYRAYGIDLSPQMLRVARHKAQRT
                     GAAPLWVARATATALPFSAATFDAVVSTFPSEYIFDPRTLREILRVLRPGGPLVIVPG
                     GVLLPVDPTSQLLQRFAALVYGQGYRRAGDHATRRQAILDALQHSPGAGPLLPNLRAT
                     GFQVQTYTGISPRSVVLVVVARKPAA"
     gene            complement(987..1892)
                     /locus_tag="VF276_12070"
     CDS             complement(987..1892)
                     /locus_tag="VF276_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607641.1"
                     /translation="MLRLPVPAVFILLVLALGWAGPAAPARAADGNVGLVISSGAGQV
                     QTYCFSVPPAGVSGLDLLQRTGLPLRVAYENLGAQICAINGVGCLDPNQPCACQCQGT
                     PCVYWQYAHWRDGRWVASTLGASSYIVKPGSIEGWGWGGNPPAPSGNALCAQAAPATA
                     TPRPPPAPTHTPRPTRPPASPTPRPLPTRPPVLTSAPPSPTIAAPATAPPVPTATPAP
                     PPPTATPVASTATAAPTDTPLPPPTVPPPATDTPAPVATVTPQVTPTTALPPADGPPA
                     TYWLFGVLALALVGVIAWARAGRRP"
     gene            complement(2043..>2420)
                     /locus_tag="VF276_12075"
     CDS             complement(2043..>2420)
                     /locus_tag="VF276_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607642.1"
                     /translation="AAAAQQAALLSNVDLSQSKPDPGRIAMPETKISDADGTLHAVPD
                     ARQVAAKGGYASVVTLGAYKGVKVDAGFGAIHAGDMLVSSPHAGYAMKADASQIKFGS
                     VIGKALADLDSGTGLIPIMVTLK"
BASE COUNT          370 a          844 c          864 g          342 t
ORIGIN      
        1 gtcgggatgg agttgcaacg agcgaaccga ggaacgaggt cgtgcggtat cggcgcgggc
       61 gccgaggttg aaacgcgcat ggcgagctat tgtccgtcgc gaccggcgta ctacaggtcg
      121 cccggctgcg tttacacata agccggcaca accggcaacc cgccacgaat ccgccgcaga
      181 ccgcggtggc acaggcgcat tgaacagacc tatctccaca caccgggcac aggaaaacgg
      241 aatgcctgcg acaccgcgcg aacaacgcat ccgcaccacg ctgctacgca cggcctttgc
      301 cctgctctat acccggttcg cctgggccta cgactgggtg agccgcacct tcttccacgg
      361 cgagtggcgg cgctggcagc gggctgcgct ggaaccgctg gcggggctgc cgggcgggcg
      421 cgtgctcgaa gtcggcttcg gcaccggcga cctgcaggcc gacctgcgcg ccgccggcta
      481 ccgcgcctat ggcatcgacc tctcgccgca gatgttgcgc gtggcgcggc acaaagccca
      541 gcgcaccggt gccgccccgc tctgggtagc gcgggccacg gccacggctc tgcccttctc
      601 cgccgctacc ttcgacgcgg tggtcagcac cttccccagc gagtatatct tcgacccgcg
      661 caccctgcgc gagatcctgc gcgtgctgcg ccccggcggc ccgctggtca tcgtgccggg
      721 cggcgtgttg ctgccggtgg accccacctc gcaactgctg caacgcttcg ccgcgctcgt
      781 ctatgggcag ggctaccggc gcgcaggtga ccacgcgacc cggcggcagg ccatcctcga
      841 cgctttacag cacagccccg gcgccgggcc gctcttgccc aatctgcggg ccaccggctt
      901 ccaggtgcaa acctataccg gcatcagccc ccggtcggtc gtcctcgtgg tcgtggcgcg
      961 caagcccgcg gcttgagcaa taatgcttac ggccgccgcc cggcccgcgc ccaggcgatc
     1021 acgcccacga gcgccagggc cagcacgcca aagagccagt aggtcgccgg ggggccatcc
     1081 gccgggggca gtgcggtggt gggcgtcacc tgcggtgtga cagtcgcgac aggcgccggg
     1141 gtgtccgtcg ccggcggcgg gacggtgggc gggggcagcg gagtatcggt aggcgcagcc
     1201 gtggccgtgg acgcgacagg tgtagcggtg ggcggaggcg gcgccggcgt agcggtcggc
     1261 accggcggcg ccgtggcggg cgcggcgatg gtggggctgg gcggcgcgga tgttaggacc
     1321 ggcgggcggg tcggcagcgg gcgcggggtc ggcgacgccg gggggcgcgt gggacgaggc
     1381 gtgtgcgtgg gtgcgggcgg aggtcgcgga gtcgcggtgg cgggcgcggc ctgggcgcaa
     1441 agcgcgttgc cgctgggcgc cggggggttg ccgccccagc cccagccctc gatcgatccc
     1501 ggcttgacga tatagctgct cgctcccagg gtcgaggcaa cccagcgccc gtcgcgccag
     1561 tgggcgtatt gccagtagac gcacggtgtg ccctggcact ggcacgcgca gggctggttg
     1621 gggtcgaggc agcccacccc gttgatcgcg cagatctgcg cgcccaggtt ctcatacgcc
     1681 acgcgcagcg gcagccccgt gcgttgcagc aggtcgaggc ccgacacgcc cgcgggcggc
     1741 acgctgaagc aataggtctg cacctggccc gccccagagg aaatcaccag gcccacgttg
     1801 ccgtccgcag cgcgcgccgg ggcggccggc ccggcccaac ccagcgcgag gaccaaaagg
     1861 atgaacaccg ccggcacagg aagccggagc aagcaggcgc ggatattcca ttgtggtggg
     1921 cagcgacaca cgcagaagta taacacagct ccgcaaccga caaaccgcca aaactaacgg
     1981 acatgaaaac gggggacacg gcgtactcgt acgccgtgtc cccctgcagt gcccgccgcg
     2041 tactacttca gtgtgaccat gatcgggatc agcccggtgc cgctgtcgag gtcggccagc
     2101 gccttgccga tgaccgatcc gaacttgatc tggctggcgt cggccttcat agcatagcct
     2161 gcgtgcgggc tgctgaccag catatctccg gcatggatgg cgccaaagcc ggcgtctacc
     2221 ttgaccccct tgtacgcccc aagcgtaacc acgctggcat agccaccctt agccgccacc
     2281 tggcgggcat cggggacagc gtgcagcgtc ccatccgcgt cgctgatctt cgtttcaggc
     2341 atggcgatgc ggccggggtc cggcttactc tggctcaggt ccacgttgga gagcagcgcc
     2401 gcctgttgcg ccgcggcggc
//
LOCUS       DASUYC010000737         8959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1137167, whole genome shotgun sequence.
ACCESSION   DASUYC010000737 DASUYC010000000
VERSION     DASUYC010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 8959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8959
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137167"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..2126
                     /locus_tag="VF276_12080"
     CDS             189..2126
                     /locus_tag="VF276_12080"
                     /inference="COORDINATES: protein motif:HMM:NF013507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEX6607643.1"
                     /translation="MSRPALALLVFVVLGAVFAAYTINQSGAQAGVPGRPAVDSWSDG
                     PALPQARKRGAAAAYPDNGKIYFFGGRGFFQRSAIDGEDRPWYNIYEYTSGTPGQWTQ
                     KNAQLPHQINAGPNGPGQTYTSNMVAATLVGPQGPAVYLVGGVNASSVPTPTVSVYFP
                     LSDSITTLNADNWPASPMRVPGGYAVLNNKLYIFGGYNHYQRKVYDDTWVFDPLAPAG
                     QRWTQLASAPLSVARAYIAGAALDGYIYAIGGDTFAEAAPPNPPNADLIPSTVVERLD
                     PSAATPQWQPVASLPTARGDMGAWSFPTGTTGGLGGSIIVGGGVWYTPDAQGYQYVAA
                     TDSWAPFADFTHGTRNYAAAQLGNRLYAIGGYNVTVVNGVTLADAAAWTQIYETSATV
                     PTPTPWPGTATPTNTPIPPTNTPVPPTNTPVPPTATPVPPTNTPLPATSTPVPTNTPV
                     PSATPCAMTFTDVTADAYYYEPVRYLACRGVISGYSDHTFRPGNVTTRGQMVKIIVLA
                     FGYAPSVPDQPTFTDVPLTHPFYSYVETAVANGIVSGYADHTFRPEADVTRGQLAKIV
                     VKAAGWRPDVPTTPTFQDVPLEDPFYGVIETAAAHGVISGYTCGSPGEPCSGPGALYF
                     RPAASATRGQIAKIVYLAVTR"
     gene            2139..3104
                     /gene="fmt"
                     /locus_tag="VF276_12085"
     CDS             2139..3104
                     /gene="fmt"
                     /locus_tag="VF276_12085"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX6607644.1"
                     /translation="MERLIFMGTPEFAVPALERLAGAGYDLTVVTQPDRPAGRGGALT
                     APPVKQTALVRGLPVWQPPTLRDPAVVARIRDLAPAAIVVVAYGEILRQSVLDIPPHG
                     CLNLHPSLLPRWRGPTPINAAILAGDTETGVSVIRMDRGMDSGPLLAQEPAPLLPDDT
                     AATLGARLAQQGATLLERTLAGVLRGTVEARPQPTEGVTICGLLRKEDGRLDWHAAAD
                     YLARQVRAYDPWPGTFTTWDGRRLKVLAAAPLPAGDPAGADAPGQVRRVAEVVPAALD
                     GTHRLAVQCGDGRLELLRLQLEGKPAMAPDALLRGYPALARAQLT"
     gene            3125..3730
                     /locus_tag="VF276_12090"
     CDS             3125..3730
                     /locus_tag="VF276_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607645.1"
                     /translation="MPTPAPAPRLPIEPASRPLYRPPTRLGCSGVTLVSIAALLAFLF
                     CLRVVAPALTQGIADLPVPRSLIGPALTATPTPEPGIPDAATPLPAPELTATLTPPAP
                     PTATVPPQATLPPAAGATAAPAAPEYVVVANTGGTGVYLRAEPRLDAARLIAVPEQTL
                     LVIAGPDTTVGSSVWRNVRTTKDPIQTGWVPAQYVTAATAP"
     gene            complement(3800..4156)
                     /locus_tag="VF276_12095"
     CDS             complement(3800..4156)
                     /locus_tag="VF276_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX6607646.1"
                     /translation="MSEELRATMRELPNVTIIDLAGDVTSFADARLHASFDTASARGL
                     KYIILNFAGVEYINSAGIATVISILAEARQRAQQLVIVGLSEHYRKIFRMVGVARYAE
                     ILDSERQALERADGVA"
     gene            complement(4187..4615)
                     /locus_tag="VF276_12100"
     CDS             complement(4187..4615)
                     /locus_tag="VF276_12100"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6607647.1"
                     /translation="MSSEKRVIHLHIPSELGYEKVAMGAARSVAQAMGFSSDRILDLQ
                     TAVAEACTNAMEHGNLLNAAQRVEVVMTVEENALAIDVNDRGANAHFGEPEIHDHNKH
                     RGMGMYIIKQLMDEVQWSSRPQGGQVRMVIYLEPKEDAEA"
     gene            complement(4683..5054)
                     /locus_tag="VF276_12105"
     CDS             complement(4683..5054)
                     /locus_tag="VF276_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX6607648.1"
                     /translation="MAQELKVTARHVDPVMVLDLQGDVTMFAEAELTRSYHLAVDNGA
                     RELVLNFAGTDYINSAGIAIIISLLTEARASGVSLVISGLSSHYQKIFRMVGLTQYAE
                     VFDTVDEAVRELKSRAAAEPA"
     gene            complement(5145..5585)
                     /locus_tag="VF276_12110"
     CDS             complement(5145..5585)
                     /locus_tag="VF276_12110"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6607649.1"
                     /translation="MPNKKRLVELRIPSIFGYEKVAMVTVMAVAARLGMSGERAEDLR
                     TAVAEACINAIEYGNREDAGTPVTITVSIDDHHLIVDVRDRALGATRPHMGRMVDLQN
                     PPSGPHANMGLFLIHHLVDKVDIVCRDNGTHVHMAMRLPEMVLH"
     gene            5911..7173
                     /locus_tag="VF276_12115"
     CDS             5911..7173
                     /locus_tag="VF276_12115"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HEX6607650.1"
                     /translation="MRYAIRAAAAAGLLVIGLAGRPLPGQAAADAHYFPETRHTVSGV
                     FWDYWQSHGGLAQQGYPITEEFQETSPIDNKPYTVQYFERAVFEYHPENKPPYQVLLS
                     PLGTLALQARYLKTAPDGQQPNRQDARTFPQTGHTLGGGFAAYWQQHGGLAQFGYPLT
                     DEFTETSATNGKPYTVQYFERAVFEYHPDVPANARIMLSLLGVSRYHARYEAVDNPRP
                     AQWPPAPGLAPTDRPVLLDATFATADLAAWQPLPTPNDPASWRMLNAHLQQDGGPDGE
                     AGTDETVLLAGDPAWTDIRLEAQFFNTSGAPTGVVWRAAQGRYYRLQLLPALPNKFPK
                     ASVQLVDGNQVRTLISVPADRFAGFEYGHWHTIGITAQGALQQVTFDGAPLVDVKDAT
                     LAAGQIGVYATADGATAFDNIRVQQLAP"
     gene            complement(7268..7423)
                     /locus_tag="VF276_12120"
     CDS             complement(7268..7423)
                     /locus_tag="VF276_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607651.1"
                     /translation="MPKIEKAVRRERQRRKAIDSQYQNVRYATQRAGEGLVRREVERT
                     TRLKKEQ"
     gene            7872..>8959
                     /locus_tag="VF276_12125"
     CDS             7872..>8959
                     /locus_tag="VF276_12125"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEX6607652.1"
                     /translation="MHIAGLALKRRTFLTLGAILGLVALVVAQVAAVIPALPTSAAQA
                     PALVAAGPGVPAVSTTFVISQFEVAGATAADEFIEIHNISGSPQDLNGHKLVYRSAAG
                     TTDATTLASWSTSTLVPAGGYYLVASATGYTGAVAPDQTYPGTTGALSGASGGIGLRN
                     GALNTGPIVDSVGYGSATNVFVETATTSVPAASTSKVRLAGGCQDTDNNSADFAVSNP
                     PVPHNSASPVAPCGAVNTPTVTVTPGGPTATPTDTATATATATPGLQVRIHDIQGAAH
                     RSPLNGAAVSNVPGIVTAKQSNGFYFQDPNPDADDATSEAVFVFGTAAAGAVNVGDAV
                     TVSGTVSEFRPGGSGGTDNLTTTEIGSPT"
BASE COUNT         1468 a         3377 c         2722 g         1392 t
ORIGIN      
        1 cgaccgctac tccgaagtac gcacccaggc caccgcccgg ctgccgaagg tcgaaatgtc
       61 gcacatcggc ggctaaaacg cacccggcgt cgccccgccc tggcgcgggt ggggcagcta
      121 taccatcgca ccgcatacac ctgcgcacac tctgacggct aaccatctat agtttgggag
      181 gagtactcat gtcgcggccg gctctggcgt tgctggtatt cgtggtactg ggggctgtgt
      241 tcgcagccta tacgatcaat caatccgggg cgcaagccgg ggttcccgga cgccccgccg
      301 tcgactcgtg gagcgacggc ccggctctgc cgcaggcccg caaacgcggc gcggccgccg
      361 cctatcccga taacggcaag atctacttct tcggcgggcg cggcttcttc cagcgctcag
      421 ccatcgacgg cgaagaccgc ccgtggtaca acatctacga gtacacctcc ggtacgcccg
      481 gccagtggac ccagaagaac gcccagcttc cccaccagat caacgccggg ccgaacgggc
      541 cggggcagac ctacaccagc aatatggtcg ccgcgacgct ggtcgggccg caggggcccg
      601 ccgtctacct ggtcggcggc gtgaacgcct ccagcgtgcc gacccccacg gtaagcgtct
      661 atttcccgct cagcgactcg atcaccacgc tgaacgccga caactggccc gccagcccca
      721 tgcgggtgcc gggcggctat gccgtgctga acaacaaact ctacatcttc ggcggctata
      781 accactatca gcgcaaggtc tatgacgaca cctgggtatt cgatcccctg gcccccgccg
      841 gccagcgctg gacccagttg gcgagcgcgc cgctgtcggt ggcgcgggcc tatatcgccg
      901 gggccgccct cgacggctac atctacgcca tcggcggcga cacctttgcc gaggcggcgc
      961 cgcccaaccc gcccaacgcc gacctgatcc cctcgaccgt cgtcgagcgg ctggacccca
     1021 gcgccgccac tccgcagtgg cagccggtgg cctcgctgcc gaccgcgcgg ggtgatatgg
     1081 gcgcctggtc cttcccgacg ggcacaaccg gcggcctcgg cggcagcatt atcgttgggg
     1141 gcggggtctg gtacacgccc gatgcccagg gctaccagta cgtcgccgcg accgattcct
     1201 gggcgccgtt cgccgacttc acgcacggca cgcgcaacta tgccgccgcg cagttgggca
     1261 atcgcctcta cgccatcggt ggctacaatg tgacggtggt caacggcgtc accctggccg
     1321 atgccgccgc ctggacacag atctacgaaa cctcggccac cgtgccgaca cccaccccgt
     1381 ggccggggac cgccacgccg acgaacacgc ccatcccgcc gaccaacacg cccgtgccac
     1441 cgacgaacac cccggtgccg cccaccgcga ccccggtgcc gccgacgaac acgccgctgc
     1501 ccgcgaccag caccccggtg ccgaccaata cgccggtgcc gagcgccacg ccgtgcgcca
     1561 tgacctttac cgatgtgacc gccgatgcct actactatga gccggtgcgc tacctggcct
     1621 gccgcggcgt catcagcggc tatagcgatc ataccttccg cccgggcaac gtgaccacgc
     1681 gcggccagat ggtgaagatc atcgtgctcg ccttcggcta cgcgccgtcc gtgcccgacc
     1741 agcccacgtt caccgacgtg ccgctcaccc atcccttcta ctcctacgtc gagacggcgg
     1801 tggctaacgg catcgtcagc ggctatgcgg accacacgtt ccggcccgag gccgacgtga
     1861 cgcgcggcca gctcgccaag atcgtcgtca aggccgccgg ctggcgcccg gacgtgccga
     1921 ccacgccgac cttccaggac gtgccgctgg aggatccctt ctacggcgtg atcgagacgg
     1981 cggcggcaca cggcgtgatc agcggctaca cctgcgggtc gcccggtgag ccgtgcagcg
     2041 gccccggcgc gctctacttc cgccccgccg catcggccac gcgcgggcag atcgccaaga
     2101 tcgtctacct ggcggtgacc cggtaggggt tcagcgtaat ggaacgcctg atcttcatgg
     2161 gcacaccgga gttcgcggtc cctgccctgg agcgcctggc cggcgccggc tacgacctca
     2221 cggtcgtgac acagccggac cgcccggccg ggcggggcgg ggccttaacg gccccgcccg
     2281 tcaagcaaac cgccctggtg cgcggcctgc ccgtctggca gccgcccacg ctgcgtgacc
     2341 ccgccgtggt ggcgcgcatc cgcgacctgg cccccgccgc tatcgtcgtc gtggcctacg
     2401 gcgagatcct gcgccagagc gtcctcgaca tcccgcccca cggctgcttg aatctgcacc
     2461 cctctttgct gccgcgctgg cggggtccca cgccgatcaa cgcggcgatc ctggccggcg
     2521 acaccgagac cggcgtgagc gtcatccgca tggaccgggg catggacagc gggccgctgc
     2581 tggcgcagga gccggccccg ctgctccccg acgacacggc ggccacgctg ggcgcccgcc
     2641 tggcccaaca gggcgcgacc ttgctggagc gcacgctggc cggcgtgctg cgcggcaccg
     2701 tcgaagcccg cccgcaaccc accgagggcg tgacaatctg cggcctattg cgcaaggaag
     2761 acgggcgcct cgactggcac gccgccgcgg actacctggc ccgccaggtg cgcgcctacg
     2821 acccctggcc tggtactttc accacctggg atggccgccg cctcaaggtg ctggccgccg
     2881 cgcccctgcc cgccggcgac cccgccgggg ccgatgcgcc cggccaggtg cgccgcgtcg
     2941 cggaagtcgt gcccgccgca ctggacggca ctcaccggct ggcggtacaa tgtggcgacg
     3001 gccggctgga actgttgcga ctgcaactgg agggtaagcc ggccatggcg cccgacgcac
     3061 tgttgcgcgg ctatcccgcg ctggcccgcg cgcaactcac ctagcccgat cctcggagga
     3121 acgcatgccg acacccgccc ccgccccccg cctcccgatc gaacccgcct cccgcccgct
     3181 ctaccgcccg cccacgcgcc tgggctgttc cggcgtgacc ctggtcagca tcgcggcgct
     3241 gctggccttc ctgttttgcc tgcgcgtggt ggccccggcc ctcacccagg gcatcgccga
     3301 cctgccggtg ccgcgcagcc tcatcggccc ggccctgacc gcgacgccca cccccgaacc
     3361 aggcatcccc gacgccgcca cgccgctgcc cgcgcccgaa ctcaccgcca cgctgacccc
     3421 gcccgccccg cccacggcga cggtcccgcc gcaggccacg ctgccgcccg cggcaggcgc
     3481 caccgctgcg cccgccgcgc ccgaatacgt cgtcgtcgcc aacaccggcg gcaccggcgt
     3541 ctacctgcgc gccgagccgc gcctcgacgc cgcccgcctg atcgccgtcc ccgagcaaac
     3601 cctgctcgtc atcgccggcc ccgacaccac ggtgggcagc agcgtctggc gcaacgtccg
     3661 caccaccaaa gacccgattc agaccggctg ggtgcccgcc caatacgtca ccgccgcgac
     3721 agcgccctaa ccgcgccggg cgccggcggg caaagaaaaa cgcccccggc aattgccggg
     3781 ggcgcagtcg ttgtagcaat caggcaacgc cgtcggcgcg ctccagcgcc tggcgctccg
     3841 agtccaggat ctcggcgtag cgggccaccc ccaccatgcg gaagatcttg cgatagtgct
     3901 cgctcaggcc gacgatcacc agttgctgcg cccgctgccg ggcctcggcc aggatcgaga
     3961 tcacggtggc gatgccggcg ctgttaatat actccacccc tgcaaagttc aggatgatgt
     4021 atttcaaccc gcgcgccgac gccgtgtcga agctggcatg cagccgggca tcggcaaaac
     4081 tggttacatc gcccgccaga tcgatgatgg tgacgttcgg tagttctcgc atcgtggcgc
     4141 gcaattcctc gctcacagga cctcctccgc gcaacctcgc gctcggttaa gcttcagcat
     4201 cttccttcgg ctccagatag atgaccatgc gcacctgccc gccctgcggc cgggacgacc
     4261 attgcacctc atccatcaac tgcttaataa tatacatacc catgccccgg tgcttgttgt
     4321 ggtcgtgaat ctccggctcc ccgaagtggg cattggcgcc ccggtcgttc acatcgatgg
     4381 ccagggcatt ctcttcgacc gtcatcacga cttccacccg ttgcgccgcg ttgagcaagt
     4441 tgccgtgctc catagcattg gtgcaggcct cggccacggc ggtttgcagg tcgaggatgc
     4501 ggtcgctact gaagcccatg gcctgggcca cgctccgcgc ggcgcccatc gccaccttct
     4561 cgtagccgag ctcactcggg atatggagat ggatcacccg cttctcgcta ctcatacgac
     4621 cccgaacctc gaaataggga agcccgcgcg cccgcacagc ggcggctggg cgcgggaacg
     4681 gatcaagccg gctcggcggc ggcgcggctc ttcaattcgc gcacggcctc gtcgaccgtg
     4741 tcaaacactt cggcatactg ggtcagcccc accatgcgga agatcttctg ataatgcgag
     4801 ctgagcccgc tgatgaccag gctgaccccg ctggcccgcg cttcggtcag caggctaatg
     4861 ataatggcga tccccgcgct gttaatataa tcggtgcccg cgaagttcag caccagttcc
     4921 cgcgcgccgt tatcgaccgc cagatggtac gagcgcgtca actcggcttc cgcgaacatg
     4981 gtcacatcgc cctggagatc gagcaccatc accggatcca catggcgggc tgttactttc
     5041 aattcctgtg ccataaatcc cattcctttc cggcgcggcc ccgccgccgt ctcgggcgcc
     5101 gctgcactcc ccgcggcgcc ccctggcacc gcgagaggcc gccgttagtg gagcaccatc
     5161 tccggcaggc gcatcgccat atgcacatgc gtgccgttgt cccggcaaac gatgtccact
     5221 ttatcgacca ggtgatgaat cagaaagaga cccatgttgg catgggggcc gctcggcggg
     5281 ttctgcaaat cgaccatgcg gcccatgtgc gggcgggtcg cgcccagtgc gcggtcgcgc
     5341 acgtccacga tcagatgatg atcgtcgatg ctgaccgtga tcgtcaccgg tgtgccggca
     5401 tcctcgcggt tgccatattc aatcgcattg atgcacgctt cggcgacggc ggttcgcaaa
     5461 tcttcggccc gctcgccgct catgccaagc cgcgcggcca cggccatgac cgtaaccatg
     5521 gcaactttct catacccgaa gatactcgga atgcgcagtt ctaccaggcg ttttttgttg
     5581 ggcaccgcga gaggtctcct gctactcgat gggagatgtg gccggcagtg ggaccccccc
     5641 tgggtgcagg accaggcaca gtccagaatc gggccttcgc cggcgggcgt gtagaatccg
     5701 aggtacgcgc gattgccgcg cgtacaaaaa atacgataac atgattcggc agccttgtca
     5761 agcgcgctgc aaacagcgct gccgttcggt atgatttgcc ccagccccga taggctacgc
     5821 cgccacgggt ggtcaaatac cccgctcatt cgttatagta ggatgtagag cgcgaacaga
     5881 ggcacagcac tcacggaaag ggacctgatc atgcgatatg cgatccgcgc cgcggccgcc
     5941 gcgggcttgc tggtgatcgg cctggccggc cggccactgc ccggccaggc cgccgccgat
     6001 gcccactact tccccgaaac ccgccacacg gtgagcggcg tcttctggga ctactggcag
     6061 agccacggcg ggctggccca gcagggctac cccatcaccg aggagttcca ggagaccagc
     6121 cccatcgaca acaagccgta cacggtgcag tatttcgagc gtgccgtctt cgagtaccac
     6181 cccgagaaca agccgccgta ccaggtgctg ttgagcccgc tgggcacgct ggccctccag
     6241 gcccgctatc tcaagaccgc gcccgacggc caacagccca atcgccagga cgctcgcacc
     6301 ttcccgcaaa ccggccacac gctgggcggg ggctttgccg cctactggca gcagcacggc
     6361 gggctggcgc agttcggcta cccgctgacc gacgaattca ccgagacgag cgccaccaac
     6421 ggcaagccct acacggtgca atatttcgag cgcgccgtct tcgagtacca cccggatgtc
     6481 ccggccaatg cgcgcatcat gctcagcctg ctcggcgtca gccgctacca cgcccgctat
     6541 gaggcggtgg acaacccgcg gcccgcgcag tggcccccgg cgcccggcct ggccccgaca
     6601 gaccgcccgg tgctgctcga cgccaccttc gcaacggcgg atctggccgc ctggcagccc
     6661 ctgcccacgc ccaatgaccc cgcttcctgg cgcatgctca acgcccatct ccagcaggac
     6721 ggcggccccg acggcgaagc cggcaccgac gaaacggtgc tgctggccgg cgacccggcc
     6781 tggaccgaca tccggctgga agcccagttc ttcaacacca gcggcgcccc caccggcgtg
     6841 gtctggcgcg cggcgcaggg gcgctactac cggctgcaac tgttgcccgc gctgcccaac
     6901 aagtttccca aggcgagcgt ccaactggtg gacggtaacc aggtgcgcac gctgatcagc
     6961 gtcccggcgg atcgcttcgc cgggttcgag tacggccact ggcacactat cggcatcacc
     7021 gcccagggtg cgctgcaaca agtcacgttc gacggcgcgc cgctggtcga cgtgaaggac
     7081 gccacgctgg ccgccgggca gatcggcgtc tacgccaccg ccgacggcgc caccgctttc
     7141 gacaatatcc gcgtgcagca actcgcgccc tgatcggcgc accgcgccca caaacaggaa
     7201 cccggattcc aatggatccg ggttcttgtt tgcatattcc gttgggtgtc gcctggcgcg
     7261 cgcatggcta ttgctccttc ttcaacctgg tcgtccgctc gacttcgcgg cgcacgagcc
     7321 cttcgccggc ccgctgcgtc gcatagcgca cattctggta ctggctgtcg atggccttac
     7381 ggcgctggcg ctcgcggcgc acggcctttt cgatcttggg catccctttc acctcctttg
     7441 ccgggcagca ttatcccgcg cgtaaccaaa ccccgccggg gcgcgatccc cggagtagaa
     7501 gccccgccct tcgcggggcg cttcctgacg gaacgcgctg gagcacggtt tccggctgat
     7561 tacaagcggc aattagtata cgttttatcc gcgcggtccg atgccccgtc gtattatacg
     7621 caatggccgg gccggcccca tccgtcgcaa ggccgatttt gcggtccgcc attgaacgtg
     7681 ggcgggtaag agcgggacca aacacacctg gcgggagcgg gctgtcgtga atcggtttgc
     7741 tgtattagcc tgccagcggg tataattctt cgccgggcac aacagcgcgc ccggtcctgt
     7801 acgggcgcac aggactctca ccttccatat cttcatcttc aggaaaccgt ctcacacaga
     7861 aggagcagat catgcacata gccggcctcg cgctgaagcg acgcacgttc ctcacgctgg
     7921 gagcgatcct gggcctggtg gccctggtcg tcgcccaggt ggccgccgtc atccccgcgc
     7981 tccccacatc cgcggcccag gcccccgcgc tcgtcgcggc cggccccggc gtgcccgccg
     8041 tctcgaccac cttcgtcatc agccagttcg aggtcgcggg cgccacggcg gcggatgagt
     8101 tcatcgagat ccataacatc agcggcagcc ctcaggacct gaacgggcat aaattggtct
     8161 accgctccgc tgccggcaca accgatgcca ccaccctggc tagttggagc acttctaccc
     8221 tcgtgccggc gggcggctat tatctggtgg cgtcggcaac cggctatacc ggcgccgtag
     8281 ctcccgatca gacctatcct ggcaccaccg gcgccttatc gggcgcatcg ggtgggatcg
     8341 gcctgcgcaa cggcgcgctg aacaccggcc ccatcgtcga ctcggtcggt tatggctcag
     8401 ccacgaacgt ctttgtggaa acggccacaa ccagcgtgcc cgccgcgagc accagcaagg
     8461 tgcgcctcgc cggcggctgc caggacaccg acaacaactc ggcggacttc gcggtcagca
     8521 atccgccggt gccgcataac agcgcgtccc cggtcgcacc gtgcggtgcg gtgaacaccc
     8581 ccaccgtcac ggtgacgccg ggcggaccga ccgccacgcc gaccgatacg gccaccgcga
     8641 cggccactgc gactcccggc ctgcaggtcc gcatccatga tatccagggc gcggcgcatc
     8701 gctcgccgct gaacggcgcg gcggtcagta acgtgcccgg catcgtcacg gccaagcaga
     8761 gcaacggctt ctattttcag gaccccaacc ccgacgccga cgacgccaca tccgaggccg
     8821 ttttcgtctt cggcaccgcg gcggccggcg cagtcaatgt cggcgatgcg gtcacggtca
     8881 gcggcaccgt ctccgagttc cggcccggcg gcagcggcgg caccgataac ctgacgacca
     8941 ccgagatcgg cagccccac
//
LOCUS       DASUYC010000738         2867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1726144, whole genome shotgun sequence.
ACCESSION   DASUYC010000738 DASUYC010000000
VERSION     DASUYC010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1726144"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..2568)
                     /gene="priA"
                     /locus_tag="VF276_12130"
     CDS             complement(10..2568)
                     /gene="priA"
                     /locus_tag="VF276_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX6607653.1"
                     /translation="MALYVEVAVDRPRPAGAPAEGDLGLILSYRIPPDIAVRPGHLVL
                     VPLGGRVTQGIAVATTDTPAVAADKVLELLRLVDPDPVLTADQLELGRWIAGYYRCSL
                     YAALALMLPPGVSSQEVVTLHLVPGGREHGGAARLTARQARVLDVLAQAGGVLPADQV
                     RRRLDGGDLNTVARQLEKRGLIERRSALVRPRVGPRHELFVRLAAPATDGKAPRLGAV
                     QSAALDWLAAPPPAGSNGEAPGDGWRAARALYRAVDGAARGTLTRLAEHGLVTLEERV
                     VWRDPLAGVRPPPYAPLLLTGRQGQVWDALAGALEARRTRKPDAPAASYLLHGVTGSG
                     KTEIYLRALGLALRLGMQAIVLVPEIALTPQTMHRFAGRFPGRVALLHSRLGAGERYD
                     QWRQIRDGGFDVVVGSRSAVFAPLPRLGLIILDEEHEWSYKQDSYPYYHTREVALKRA
                     ALTGAVVVLGSATPDVATYHRATRPPVTPDPVPGVQWDLLSLPARVGLGRGPDGGEVT
                     TELPMPPVRVVDMRQELRGGNRSIFSMALQQALAATLRAGEQAILFLNRRGSRTFILC
                     RACGYVPTCPQCDIPLVYHADIDLLLCHRCDLHKREPHPCPRCGSPSIKRFGAGTQRV
                     EEEMRHLFPDARVLRWDRDTAARKGAHDDLLGAFVRQEADVLVGTQMIAKGLDLPLVT
                     LVGVISADTGLHLPDFRAGERTFQLLTQVAGRAGRRTAGGQVIVQTYSPEHYAIQAAA
                     RHDYRSFYTQDIRFRLAHEYPPFSRMAKLVYSAYSEAACQIEANRLAGVILDLLAGLD
                     EEIEVLGPAPSFMHKLRNRYRWQLLLRGPDLAPLLAQLPLPPGWTLDIDPVSML"
BASE COUNT          419 a         1004 c         1055 g          389 t
ORIGIN      
        1 cgtgggcgat taaagcatac tcaccgggtc gatgtcgagg gtccagccgg ggggcagagg
       61 gagttgggcc agcagcgggg cgagatcggg gccacgcagc agcaactgcc agcgatagcg
      121 gttgcgtagc ttgtgcatga acgagggcgc cggacccagc acctcgatct cctcatctag
      181 ccccgcgagg aggtccagga tcacgccggc caggcggttg gcttcgatct ggcaggccgc
      241 ctcagagtag gcgctgtaga ccagcttcgc catgcggctg aagggcgggt actcgtgcgc
      301 caggcggaag cggatgtcct gcgtgtagaa cgagcggtag tcgtggcggg ccgccgcctg
      361 gatcgcgtag tgctcggggc tgtaggtctg gacgatcacc tggccgcccg ccgtgcgccg
      421 cccggcccgc ccggccacct gggtcagcag ttggaacgtg cgctcgcccg cgcggaagtc
      481 gggcaggtgc aggccggtgt ccgcgctgat cacgccgacc agcgtcacca gcggcaggtc
      541 gaggcccttg gcgatcatct gcgtgccgac cagcacatcg gcctcctggc gcacgaaggc
      601 gccgagcagg tcgtcgtggg cgcccttgcg ggcggcggtg tcgcggtccc agcgcagcac
      661 gcgcgcatcg gggaagaggt ggcgcatctc ctcctcgacc cgctgcgtgc ccgcgccgaa
      721 gcgcttgatg ctgggcgaac cacaacgcgg gcagggatgt ggctcgcgct tgtgcaggtc
      781 gcagcggtgg cagagcagca ggtcgatgtc ggcgtggtag accagcggga tgtcgcactg
      841 cgggcaggtg ggcacgtagc cgcaggcccg gcagaggatg aaggtgcggc tgccgcgccg
      901 gttgagaaac aggatagcct gctcgcccgc gcgcagggtg gccgccagcg cctgttgcag
      961 cgccatgctg aagatcgagc gattgccgcc gcgtagctcc tggcgcatat cgaccacgcg
     1021 cacgggcggc atcggcagtt cggtggtcac ttcgccgccg tcggggccgc gccccaggcc
     1081 gacgcgggcg ggcagactca gcaggtccca ttgcacgccg ggcaccgggt cgggcgtgac
     1141 gggcgggcgc gtggcccggt ggtaggtcgc cacgtcgggc gtggcgctgc cgagcacgac
     1201 gaccgcgccg gtgagtgcgg cccgcttgag cgctacctcg cgcgtgtggt agtaggggta
     1261 gctgtcctgc ttatacgacc actcgtgttc ctcgtcgaga atgatcagcc cgaggcgcgg
     1321 cagcggcgcg aagacggcgg agcgcgaccc gaccaccacg tcaaagccgc cgtcacggat
     1381 ctggcgccac tggtcgtagc gctcgcccgc gccgaggcgc gagtgcagca gggccacacg
     1441 ccccggaaag cgcccggcga agcggtgcat ggtctgcggg gtcagcgcga tctcgggcac
     1501 cagcacgatg gcctgcatcc ccaggcgcaa ggccaggccc agcgcacgca ggtagatctc
     1561 ggtcttgccg ctgccggtca cgccgtgcag caggtacgac gcggcggggg catcgggctt
     1621 gcgcgtgcgc cgggcttcca gcgccccggc cagcgcgtcc cagacctggc cctggcgccc
     1681 cgtcagcagc agcggcgcat acggcggcgg gcgcaccccg gccagcggat cgcgccacac
     1741 cacccgctct tcgagcgtga ccagcccgtg ctcggccagc cgggtgagcg tgccgcgggc
     1801 agcgccgtcc accgcccgat agagcgcgcg cgccgcgcgc cagccgtcgc cgggcgcctc
     1861 gccattgctg cccgccgggg gcggcgcggc cagccagtcg agcgcggcgc tctgcacggc
     1921 gcccagccgg ggcgctttgc cgtccgtcgc gggcgccgcc aggcggacga acaactcgtg
     1981 gcgcggtccc acgcgcgggc gcaccagggc cgagcgccgc tcgatcaggc cgcgcttctc
     2041 cagttgccgg gccaccgtgt tcaggtcgcc gccgtccagg cgccgccgca cctggtccgc
     2101 gggcaacacg ccgcccgcct gcgccaggac atcgagcacc cgcgcctggc gggcggtcag
     2161 ccgtgcggcc cccccgtgtt cgcggccgcc cggcaccagg tgcagcgtga ccacttcctg
     2221 gctgctcacc cccggcggca acatcaaggc cagggcggcg tacagcgagc agcggtagta
     2281 gcccgcgatc cagcggccca gttcgagctg gtccgccgtg agcacgggat ctggatcgac
     2341 caggcgcagc agttcgagga ctttgtcggc ggcgacggcg ggggtatcgg tggtggcgac
     2401 ggcgatgccc tgcgtgaccc gcccgccgag gggcaccagc accaggtggc cggggcgcac
     2461 cgctatgtcc gggggaatgc ggtagctgag gatgaggccc aggtcgccct cggcaggcgc
     2521 ccccgccggg cgcgggcggt cgaccgctac ctctacatat aatgccacac gctcaccacc
     2581 catcgccaac ggctaccgtt gcccgattat acccaaaacc ggcgctactt tacaaaccgg
     2641 aatcgctgtg ctagaataga cataactacg cgtgcgcgca tgctttctaa cttgcgccgc
     2701 cggcggcact tccccctgat tcctgactgg cgattcatct cgggggcagt ggcccggcag
     2761 tgtggcgcag cttaactttg attattacag gcccgcagca cccgcgggcg ccggcgatgg
     2821 ccggcggtga cttgcctgcg gacgggcggg cgagcaggac caggagc
//
LOCUS       DASUYC010000739         2898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_574045,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000739 DASUYC010000000
VERSION     DASUYC010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574045"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VF276_12135"
     CDS             complement(<1..306)
                     /locus_tag="VF276_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607654.1"
                     /translation="MNYSLAQLLEEQLPFLAAQWPEPETDSTDRPADSPASSFGLLHY
                     RQLLDAVHKDDYTAWHDQLRREGAAWAVAGSEVGTLIAGAQGFMNAVRRAVLADRLAP
                     "
     gene            486..1664
                     /locus_tag="VF276_12140"
     CDS             486..1664
                     /locus_tag="VF276_12140"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX6607655.1"
                     /translation="MAKNISPGREATDQPPPINAPFTRRQVLSLVIPGVLLLALIGVL
                     LWGALSTVRAPAGAAAPPDTATLGGPATPATVPAAAGTDAATATPQATATVAAPSDSP
                     TAGPTETPAAPATATTAPTEPPAATSTAAPAATDAPAATPSPAADNADRPLQGRRIGL
                     DPGHGPRNDLGAVLVDKATGKLILSEDELNLDVALRCRDILQARGATVIMTRETKDSF
                     TAPWPADVNGDGIKHGQADDLQERIDILNEGHAEVFLSIHANSSANPARRQGIQGLYC
                     ATDECPFPKQSKRLGLLVMDQLEAKLAEAGNPITQRELRSDLWSDGPNDPYKHLFLLA
                     PAEPPSHPRGTHMPGLIVESLYVTSPTEAAQLKQDRVRQAIALAYADALQQYLTEPAP
                     "
     gene            1713..2390
                     /locus_tag="VF276_12145"
     CDS             1713..2390
                     /locus_tag="VF276_12145"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX6607656.1"
                     /translation="MPVFDGPDRLYLVRHGETHSNREQRFQGQLDDPLSLAGQAQAGA
                     LGRWLAGRPVRFGALYSSDLRRAAETAATVGAHLGLTPVLAPGLREIDCGEWQGLTGP
                     EIEVRYPGGLQMWLDEVATYCLPGGESVPDLQRRILAFYQDTVAPRRDEAVLLVSHGL
                     ALSVLIAPLLGWDLVEAWRERRAGMANTGVTVLARDPARGAYRLDLLNSTEHLPRTTD
                     LPATVIG"
     gene            2480..2818
                     /locus_tag="VF276_12150"
     CDS             2480..2818
                     /locus_tag="VF276_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010144219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEX6607657.1"
                     /translation="MPIYEYECRECGRRFERMQSFHDEPIRVCPHCGGETRRVFHPVG
                     VIFKGSGWYVTDSRKSPSESANSTGTSGDKGDKAASTGSKDGEAPSSAPAAKDSTSPA
                     PAAPKTGSEA"
BASE COUNT          479 a         1022 c          965 g          432 t
ORIGIN      
        1 gggggctagc cggtcggcca gcacagcgcg gcgcacggcg ttcatgaagc cctgcgcccc
       61 ggcgatcagc gtgccgacct cgctgccggc caccgcccag gccgcgcctt cccggcgcaa
      121 ctgatcgtgc caggccgtgt aatcgtcctt atgcaccgcg tccagcaact ggcggtagtg
      181 gaggaggccg aaggaggaag cgggggaatc ggcgggccgg tcggtcgaat ccgtttccgg
      241 ctccggccac tgggcggcca ggaacggcag ttgctcttcc agcaattggg cgagcgaata
      301 gttcatgtcc ctactcgaat ttccttcctc ttgcaaatac gggcgacatc gacgggccgg
      361 caggcactct tgtattatat cataaatcgg cgccgcttga cgccgcaccg cttccttgtc
      421 tatactgcgt agggccggag tgccggcctg tttatcccac atcctaacga cgattgcatg
      481 aggttatggc aaaaaatata tctcctgggc gggaggcgac cgatcaaccg cctccgatta
      541 acgcgccgtt tacccgccgc caggtcctga gtctggtcat ccccggcgtg ctgctgctgg
      601 ctctcatcgg cgtgctgctg tggggcgcgc tgtctacggt gcgcgccccg gcgggagcgg
      661 cggccccgcc ggacaccgcc accctcggcg gccccgccac tcccgccacc gtgcccgcag
      721 ccgccggcac cgatgccgcc accgcgacgc cgcaggcgac cgccaccgtg gcggcaccat
      781 ccgactcccc gaccgccggg cctacggaga ccccggcggc cccggcgacg gcgaccacgg
      841 cgcctaccga accgcccgcg gcgacctcaa cggcggcccc ggcggcgacc gatgcacccg
      901 cggccacgcc tagcccggcg gcggacaacg cggatcgccc gctgcaaggc cggcgtatcg
      961 gcctcgaccc tgggcatggc ccgcgcaacg acctgggcgc tgtgctggtc gataaggcca
     1021 ccggcaagct gatcctgtcc gaagacgaac tgaacctcga tgtggcgctg cgctgccgcg
     1081 atattttgca ggcgcgtggc gcgaccgtga tcatgacgcg cgagaccaaa gacagcttca
     1141 ccgcgccctg gcccgccgat gtcaacggcg acggcatcaa gcacggccag gccgacgacc
     1201 tgcaagagcg catcgacatc ctcaacgagg gccatgccga ggtcttcttg agcattcacg
     1261 ccaacagcag cgccaacccg gcgcggcgcc agggcatcca gggcctctat tgcgccaccg
     1321 acgaatgccc cttccccaag cagagcaagc ggttgggctt gctggtcatg gaccagcttg
     1381 aggccaagct ggccgaggcg ggcaacccga tcacccagcg cgagttgcgc agcgacctct
     1441 ggtccgacgg gccgaacgac ccgtacaagc acctgttcct gctggcgccc gccgaaccgc
     1501 cgagccaccc gcgtggcacc cacatgccgg gcctgatcgt cgagtcgctc tatgtcacct
     1561 cgcccaccga agccgcgcaa ctcaagcagg atcgcgtgcg ccaggccatc gcgctggcct
     1621 acgccgacgc gctccagcaa tatctgaccg agcccgctcc ctagccgggc ggatgattgc
     1681 aggccccggc ctgccctgga atggatcgat tcatgcctgt ttttgatggg ccggaccggt
     1741 tgtacctggt ccggcatggc gagacccact cgaaccgcga acagcgcttc cagggccaac
     1801 tggacgatcc cctgagcctg gccggccagg cccaggccgg cgcgttgggt cgctggctgg
     1861 cgggccgtcc ggtgcgcttc ggcgcgctct acagcagcga cctgcgccgc gccgcggaga
     1921 ccgccgcgac cgtcggggcg cacctcggcc tcacgccggt gctcgcgccc ggcttgcgcg
     1981 agatcgattg cggcgaatgg cagggcctga ccgggcccga gatcgaggtc cgctatccgg
     2041 gcgggctgca aatgtggctc gacgaggtcg cgacctactg cctgcccggt ggcgaaagcg
     2101 tgcccgacct gcaacggcgc atcctggcct tctaccagga tacggtggcc ccccggcgcg
     2161 acgaggccgt gctgctggtc tcgcacgggc tggccctgtc ggtgctgatc gcgccgctgc
     2221 tgggttggga cctcgtggaa gcctggcggg agcgccgcgc cgggatggcc aataccggcg
     2281 tcaccgtgct ggcccgcgat cccgcgcgcg gcgcctaccg cctggatctg ctcaactcca
     2341 ccgagcatct gccgcgtacg accgacctgc ccgcgacggt catagggtga tgctgtataa
     2401 taagggatcg taatggccgg agcagtggac tctaaattgc agaattaccg gcagaatcca
     2461 gtgtcgcgga ggagtcagga tgccgattta tgagtatgag tgccgcgagt gcggccggcg
     2521 gtttgagcgg atgcagtcgt ttcacgacga gccgattcgc gtctgcccgc actgtggcgg
     2581 cgagactcgg cgcgtgttcc acccggtggg ggtgattttc aaggggtcgg gctggtacgt
     2641 caccgacagc cgcaagtccc ccagcgagtc cgcaaattcg accggcacga gtggcgacaa
     2701 gggcgataag gcggcgagca cgggcagcaa ggatggcgaa gcgccgagca gcgccccggc
     2761 cgccaaggac agcacatccc cggccccggc ggcgcccaag accgggagtg aggcatagcg
     2821 ccggagaggc cgcagtatga ccgatctgtt gtatgggcgc aacgcggtgc gcgaagccct
     2881 gcgcgccggg cgcaaagt
//
LOCUS       DASUYC010000740         3106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1775807, whole genome shotgun sequence.
ACCESSION   DASUYC010000740 DASUYC010000000
VERSION     DASUYC010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1775807"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="VF276_12155"
     CDS             <1..564
                     /locus_tag="VF276_12155"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607658.1"
                     /translation="YLPGQGIKARSEGLAISRQYYLMPTPGSNGQPQQVSSVQAGDLV
                     RVRLSIVVPETSYYLAVEDPLPAGLEAVNGSLGTTADSERPPNPSGTREPGVAPGKRL
                     PPGVDTVESYFPWWLRWGPFSNVEMRDDRVALFANSISPGTYVYEYYARATTPGAYMA
                     QPAQARLLYFPDVFGRSDGGVFTINAR"
     gene            complement(642..1535)
                     /locus_tag="VF276_12160"
     CDS             complement(642..1535)
                     /locus_tag="VF276_12160"
                     /inference="COORDINATES: protein motif:HMM:NF004843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6607659.1"
                     /translation="MKDFTGKVAVVTGAASGIGRALADRGAQEGMKVVLADIEQSALD
                     RAAAEIAATGAAVLAVRTDVSKAEDVDALARKAVDTFGGVHLLFNNAGVGAGTTAWGS
                     SLRDWEWVLGVNLWGVIHGLHSFVPLMLSQGGEGHIVNTASIAGLVSGPGLAAYKVSK
                     HGVVSLSETLYAELQQAGAPIGVSVLCPFWVQTRIMDAERNRPAALQNAPADQDIPPE
                     IAAGVAAMRQAVDQGIPPEQVAEAAFAAIRDGQFYVVVPAEAYPIAQARAADILYHRN
                     PGDTAREHEAMAASPEATPAG"
     gene            1778..2389
                     /locus_tag="VF276_12165"
     CDS             1778..2389
                     /locus_tag="VF276_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HEX6607660.1"
                     /translation="MFDLFGRGPKSHYAPDELKKRLPPGQSLTEKWPVLHYGPVPHRR
                     LEDWDFRIWGLVEQPVRWTWDEFMALPKISLTRDISCVTRWTRFDLNFEGVPFKEVMK
                     HVKLKPNAKFVMIHAENGFTTNLPLADLMRDDVIFAHKAEGHELELEHGWPLRLVVPH
                     LYFWKSAKWVRGLEFMAEDAPGFWEEAGYHMRGDPWKEERYGW"
     gene            complement(2552..>3106)
                     /locus_tag="VF276_12170"
     CDS             complement(2552..>3106)
                     /locus_tag="VF276_12170"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX6607661.1"
                     /translation="IGYFAMGLRYWPVLAVIIFVCEAIPLVGPYIGTAPAVLVALTQP
                     GNDGLPLLLGMDNLGSVTRALLVIVFAVVLQMVEGNVLVPRIMRNSVGISALTVIVSL
                     LIGSALAGLAGALLAVPVAGAIQVIIMDLQAAATARAEAEKRAAQEAVDKEHLEHIIV
                     PSDETAHTANDPPKLIVPGAAGEQ"
BASE COUNT          521 a         1108 c         1000 g          477 t
ORIGIN      
        1 tacctgcccg gccagggcat caaggcgcgc agcgagggcc tggcgatctc gcgccagtac
       61 tacctgatgc ccacgccggg cagcaacggc cagccccagc aggtcagcag cgtgcaagcg
      121 ggtgatctgg tccgggttcg gttgagcatc gtggtgccgg agacgagcta ctacctggcc
      181 gtcgaagacc cgctgcccgc cgggctggaa gccgtcaacg gctcgctggg caccaccgct
      241 gacagcgagc gcccgccgaa ccccagcggc acgcgcgaac cgggcgtggc gcccggcaag
      301 cggctgccgc cgggcgtgga caccgtggag tcttacttcc cgtggtggct gcgctggggg
      361 ccgttcagca acgtcgagat gcgcgacgac cgcgtggccc tgttcgccaa ctcgatcagc
      421 cccggcacct acgtctacga atactacgcg cgggccacca cgcccggcgc ctacatggct
      481 cagccggccc aggcccgcct gctctacttc ccggatgtct tcgggcgcag cgacggcggc
      541 gtcttcacca tcaacgcgcg ctaactcagc gtacgttcaa gcgccacggc gcgggatcgg
      601 acatgatccc gcgccgtggc gctttccgca cccgcccgtg tctagccggc cggggtcgcc
      661 tcgggcgagg ccgccattgc ttcatgctcg cgcgccgtgt cgccggggtt gcggtggtag
      721 aggatgtcgg ctgcccgcgc ctgggcgatg gggtacgcct cggcggggac gaccacgtag
      781 aactgaccgt cgcggatcgc ggcgaaggcg gcctcggcca cctgctccgg cgggatgccc
      841 tggtccaccg cctggcgcat cgccgcgacc cccgccgcga tctccggggg gatatcctgg
      901 tcggccggcg cgttctgcag ggcggccggg cggttgcgct cggcatccat gatccgggtc
      961 tgcacccaga acgggcagag caccgatacg ccgatcggcg cccccgcctg ctgcagttcc
     1021 gcatagaggg tctccgacag gctgaccacg ccatgcttgc tcactttata ggccgccagc
     1081 cccggacccg agacgaggcc ggcaatcgag gcggtgttga cgatatgccc ttcgccgccc
     1141 tggctgagca tgaggggcac aaagctgtgc aggccgtgga tgacgcccca caggttgacg
     1201 cccagcaccc actcccagtc gcgcaggctg ctgccccagg ccgtggtgcc cgcgcccacc
     1261 ccggcgttat tgaacagcag gtgcaccccg ccgaaggtgt ctaccgcctt gcgggccagg
     1321 gcatccacat cttcggcctt cgacacatcg gtgcgcacgg caaggaccgc ggcaccggtg
     1381 gccgcgatct ccgcagcggc ccgatccagg gcgctctgct cgatatcggc caggaccacc
     1441 ttcatgccct cctgggcgcc ccgatcggcc agcgcacggc caatgccgct ggcggccccc
     1501 gtcaccacgg cgacttttcc cgtgaagtcc ttcatgtctg cctcctattg gcgaaagcga
     1561 cggcctatag tccgccgggc aaggggcaaa cctcgcccga tattcccgat cagtatactc
     1621 catcgcgggc agttgttctg ttacccgcaa tgggcccata catccggcac cggcttgggc
     1681 cacgttctaa gaaacatgta gtagcacaaa tcactgccgc gcccgaccgc gtgcgggttc
     1741 cggacccacg gcggcggacg gcaaggagca aaagtctatg tttgacctgt ttgggcgcgg
     1801 ccctaagagc cactacgccc ccgatgaact gaaaaagcgc ctgccaccgg gccagagcct
     1861 gaccgagaag tggcccgtgt tacactacgg cccggtgccc catcgccggt tggaagactg
     1921 ggatttccgc atctggggcc tggtcgagca gccggtgcgc tggacctggg acgagttcat
     1981 ggcgctgccc aagatctcgc tcacccgcga catcagttgc gtcacccgct ggacccgctt
     2041 cgacctcaac ttcgagggcg tgcccttcaa agaggtgatg aagcacgtga agctcaagcc
     2101 gaacgcgaag ttcgtcatga tccacgccga gaacggcttc accaccaacc tgccgctggc
     2161 cgacctgatg cgcgacgacg tgatcttcgc gcacaaggcc gagggccacg aactggagtt
     2221 ggagcacggc tggccgctgc gcctggtggt gccgcacctc tacttctgga agagcgccaa
     2281 gtgggtgcgc ggcctggagt tcatggccga ggacgcaccg ggcttctggg aggaagccgg
     2341 ctaccatatg cgcggcgacc cctggaaaga ggagcgctac ggctggtagg cccggcccct
     2401 cccgcgccgc atacacatac gcacagcaac agggcggacg ctccggcgcc cgccttagct
     2461 gtttggtgca gccgcgcggg ccggtttcaa accgcggccc ctacaccaac aacctactga
     2521 ccactgacaa ccgatcactg actactaacc gctactgctc ccccgcggcg cccggcacga
     2581 tcagcttggg cgggtcgttc gcggtgtggg ctgtctcgtc gctgggcacg atgatgtgtt
     2641 ccaggtgctc cttgtcgacg gcttcctgcg ccgcgcgctt ctcggcttcg gcgcgggccg
     2701 tggcggcggc ctgcaagtcc atgataatga cctggatggc cccggcgacg ggcacggcca
     2761 gcaaggcccc ggccagcccg gccagcgcgg agccgatcaa caggctcacg atcacagtca
     2821 gcgcgctgat gccgacgctg ttgcgcatga tgcgcggcac cagcacgttg ccctcgacca
     2881 tttgcagcac cacggcgaac acgatcacca gcagcgcccg cgtgacactg cccaggttgt
     2941 ccatgcccag caacagcggc aggccatcgt tgcccggctg ggtcagcgcc accagcacgg
     3001 cgggcgccgt gccgatgtag gggccgacca gcggaatagc ctcgcagacg aagatgatca
     3061 ccgccagcac cggccagtag cgcaggccca tcgcaaaata gccgat
//
LOCUS       DASUYC010000741         3020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_343716,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000741 DASUYC010000000
VERSION     DASUYC010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343716"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VF276_12175"
     CDS             complement(<1..327)
                     /locus_tag="VF276_12175"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6607662.1"
                     /translation="MELKKSDQILHPVRLQILLALGRNALTTHEIADRLPDVPKSSIY
                     RHLKVLLDGGIIQVAATRPVKGIEEKVYAMPAPAHVGPDDLASVSADEHLRYFTTYVT
                     ALLQQFA"
     gene            complement(452..1432)
                     /locus_tag="VF276_12180"
     CDS             complement(452..1432)
                     /locus_tag="VF276_12180"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX6607663.1"
                     /translation="MNTATGTQLDRKGILGFLAVAFGFTWLIDLVLFVTGQGITGPWS
                     LPLIAVSMFGPALATFVVTHWISPIRNIRVATGLRLGAKGTRWGWYYLLATVGMLIIT
                     LAGLFLAAAVGVYHMDLTNYSAYRAALEAVPGAGAALAGTPLGVLVWAQILSLPIACI
                     INAPVIFGEEYGWRGYLLPALLPLGQWPALILQGLIWGFWHAPLILMGYNYPGHPFLG
                     VIMMMLATTVVGILIGWTRLATGSVWPAVLAHALINGGAQIPMLLSQAGTPVDTLHIS
                     MLGWSGMVVELVAIGILVLLRRLPVRQAPDLAEPAIPADAGVPNAHTATA"
     gene            complement(1631..1861)
                     /locus_tag="VF276_12185"
     CDS             complement(1631..1861)
                     /locus_tag="VF276_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607664.1"
                     /translation="MESLLSGMCLLLLVLGGAVLWLFGLLVRLVNRVATLSDEVETLR
                     RESDPADLGCGGLLAAAVVLVGIVVVAGAWLR"
     gene            2079..>3020
                     /locus_tag="VF276_12190"
     CDS             2079..>3020
                     /locus_tag="VF276_12190"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NB-ARC domain-containing protein"
                     /protein_id="HEX6607665.1"
                     /translation="MQSETFGRLLKAGLSSVAACEGKTAPIVEDELGRQIGVAATSIQ
                     RYKAGHIPPETRTVAILAEAAVKRGHLNCDWLAAFLHAARYPTAERLLEQLCPPAAPQ
                     VRLARIYQNLPAPTYSQFVMRAQAYADVVDGLAQRSALVLIASLGGMGKTSLAREVAI
                     RCLAAESPVRFDAVVWVSDKDRPGSTTLPGLLDEIARTLDYPGFTQFEGEEKRREVEQ
                     LLRRQRVLLVVDNFETVTDGSLLPWLLRLPEPSKALITSREYSRAFRNNTFVLDLRGM
                     SEAEAQELIAQRLRLLRIDTLVRDPTELAPLVAATGGN"
BASE COUNT          564 a         1051 c          965 g          440 t
ORIGIN      
        1 cgcgaactgc tgcaacaggg cggttacata ggtggtgaag tagcgcaggt gctcgtccgc
       61 gctcacgctc gccaggtcat ccggcccgac atgcgcgggc gcgggcatgg cgtagacctt
      121 ctcctcgatg cccttgaccg ggcgcgtggc cgccacctgg atgatgccgc cgtcgagcag
      181 caccttgagg tgccggtaaa tcgacgattt gggcacgtcc ggcagccggt cggcgatctc
      241 gtgcgtggtc aacgcgttcc tgccgagcgc cagcaagatc tgcaagcgca ccgggtggag
      301 aatctgatca cttttcttga gttccatgcg gcattcctcg ttcccatgat accagcattg
      361 gcccggcgcg acaatcgccg taagccggga ggaacaagac agggcacgca gcgggtgctg
      421 ggtgccctgt cgccaggcca ttttctggag gttatgccgt cgccgtgtgc gcgttcggca
      481 ccccggcatc cgccgggata gcgggctcgg ccaggtcggg cgcctggcgc accggcaggc
      541 gccgcagcag gaccaggatg ccgatggcga ccaactccac taccatgccg ctccaaccca
      601 gcatcgagat atgcagcgtg tccaccggcg tgccggcctg agacagcagc atcgggatct
      661 gcgcgccgcc gttgatcaac gcgtgggcca gcaccgcggg ccagacgctg ccggtcgcca
      721 gccgcgtcca gccgatcagg ataccgacga ccgtggtggc cagcatcatc atgatcacgc
      781 cgaggaacgg gtggcccgga tagttatagc ccatcaggat gagcggcgcg tgccagaagc
      841 cccagatcag gccctgcagg atcagcgccg gccactggcc cagcggcagc agcgcgggca
      901 gcaggtagcc gcgccagccg tactcctcgc cgaagatcac cggcgcgtta atgatgcagg
      961 cgatcggcag gctgaggatc tgcgcccaga ccagcacgcc cagcggcgtg cccgccagcg
     1021 cggcccccgc ccccggcacc gcctccagcg ccgcccggta ggccgagtag ttggtcagat
     1081 ccatatgata gaccccgacc gcggcggcca ggaacagccc ggccagcgtg ataatcagca
     1141 tgccgaccgt cgccagcagg taataccagc cccagcgcgt gcccttcgcg cccaggcgca
     1201 ggccggtcgc cacgcggata ttgcggatcg ggctgatcca gtgtgtgacc acgaaggtcg
     1261 ccagggccgg gccgaacatc gatacggcaa tcagcggcag cgaccacggt cccgtaatcc
     1321 cctgcccggt cacaaagagg accagatcga tcagccaggt gaacccgaaa gccacggcca
     1381 ggaagccgag aatgcccttc cggtcgagtt gtgtaccagt agcggtattc atcgcagtat
     1441 gtctcatcct tattcaatag cggtcaatag cattgtaacg atgccgcgct ggtcggcgct
     1501 taccccttgc cggcgcgctc gctatcgtta ttaataataa taacgattgc ataatcgata
     1561 gacaaccgac tttagttgga tattatccgg caaaacgtcc caccagggag cgggggaaac
     1621 cgccggagcg ctagcgcagc cacgccccgg cgacgaccac tatcccaacc agaaccaccg
     1681 ccgcagccag caacccgccg cagcccaggt ccgccgggtc gctctcgcgg cgcaacgtct
     1741 ccacctcgtc gctcagcgtg gcgacgcggt tcaccaggcg gacgagcagt ccgaacagcc
     1801 agagcaccgc cccgcccaac accagcaaca gcaaacacat cccgctcagc aaactttcca
     1861 tggctctgcc ctcctgacgg cgtggtgcga caaaaaagcc gaagcgcgcc cgctttcatt
     1921 acaccgccat cacaccacca tccgctgcgg aagggggcgg tacccggtgc ggaagtttcc
     1981 gcgctgaatc ttccgcgccg ggcgcgggac atcccgcagg agaatgggct ataatgatga
     2041 ggtcgattgc aacgggggcg cggcaagggg gaagcggcgt gcagagcgag acgttcgggc
     2101 ggttgctgaa ggccgggtta agcagtgtcg cggcgtgcga aggcaagacc gcgcccatcg
     2161 tggaagacga actgggccgg cagatcggcg tagccgctac gtcgatccag cgctacaagg
     2221 ccgggcacat cccgccggaa acgcgcaccg tggcgatcct ggccgaggcc gccgtcaagc
     2281 gcggccacct gaactgcgac tggctggccg ccttcctgca cgcggcccgc taccccaccg
     2341 cggagcgcct gctggagcaa ctctgcccac ccgccgcccc gcaggtccgc ctggcgcgca
     2401 tctaccagaa cctgcccgcg ccgacgtata gccagtttgt tatgcgcgcg caagcctacg
     2461 ccgacgtggt cgacggcctg gcccagcggt cggcgctggt gctgatcgcc agcctgggcg
     2521 gcatgggcaa aaccagcctg gcccgcgagg tcgccatccg ctgcctggcc gccgagagcc
     2581 cggtccgctt cgacgcggtg gtctgggtga gcgacaagga ccgccccggc agcaccacgc
     2641 tgcccggctt gctggacgaa atcgcgcgca cgctcgacta ccccggcttc acccagttcg
     2701 agggcgagga gaagcggcgc gaggtggagc agttgctgcg ccgccagcgc gtgctgctgg
     2761 tggtcgataa cttcgagacc gtgaccgacg gcagcctgct gccctggctg ctacgcctgc
     2821 ccgagccgag caaggcgctg atcacgagtc gcgaatactc gcgcgccttc cgcaataaca
     2881 cctttgtgct cgacctgcgc ggcatgagcg aggccgaagc ccaggaactg atcgcccaac
     2941 ggctgcgcct gctgcgcatc gacaccctgg tgcgcgaccc caccgaactg gccccgctgg
     3001 tggcggccac cggcggcaat
//
LOCUS       DASUYC010000742         4668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1266667, whole genome shotgun sequence.
ACCESSION   DASUYC010000742 DASUYC010000000
VERSION     DASUYC010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4668
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266667"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VF276_12195"
     CDS             complement(<1..396)
                     /locus_tag="VF276_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607666.1"
                     /translation="MPPRRLALFGFTLPVLLLTASLRSAPAESPPAPVARPLPAPATE
                     RVSLAANGAQAPDMSSGPAISGDGRFVAFESVAALVPTDTNGLPDVYVRDRLLGTTIW
                     ASVPLTGTATGPSSAAAISADGRFVAFQSA"
     gene            complement(486..1280)
                     /locus_tag="VF276_12200"
     CDS             complement(486..1280)
                     /locus_tag="VF276_12200"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX6607667.1"
                     /translation="MSSNPDYPVADAAEDLPTDDAPAYGTLPRRRVRAAAETPEIDDS
                     IHTEPAETDPYAYEYEEAPAPRGSMVPIVISVVAAVVVIVLMAAIMLTPRSPATPAAA
                     GAAGASSTINVGTPAGGGKESPRVGYLAPDFTLTGLDGGTVQLSSYRGVKPVWVNFWA
                     TWCPPCRAEMPEMEKLYPAYQAKGIEILGVDVQESQAQVKQFVQQGGYSWKFALDLDG
                     KASRAYYVTGIPTHIFVGRDGVIRDLVVTGLDRASMESKLNKLLTQ"
     gene            complement(1421..2056)
                     /locus_tag="VF276_12205"
     CDS             complement(1421..2056)
                     /locus_tag="VF276_12205"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX6607668.1"
                     /translation="MSDETTSLDSSEPYSASLDSPEPYSAAANPAHAARISIIVAAAS
                     GLLVLGLLVSIILTPSKPATAGVPEAMSIGSLAPNFSLQRLEGGNVQLSSYRGVKPVW
                     VNFWATWCPHCAVEMPEMQQFYKQYQARGLEILGVDDQESAQLVRPYITQGGYTWSFL
                     LDLDGKITRQYRITGLPTHVFIGRDGVIQNIVVGSITREQMDAAVTKLMSQ"
     gene            complement(2220..2456)
                     /locus_tag="VF276_12210"
     CDS             complement(2220..2456)
                     /locus_tag="VF276_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607669.1"
                     /translation="MPNSVTLAVPDALLQRARAEAALTNRSVDAVLVDWLQRGAPPPD
                     PSYVIYTPSGLDAAAQRLLDALAADKLEDPASES"
     gene            2644..3525
                     /locus_tag="VF276_12215"
     CDS             2644..3525
                     /locus_tag="VF276_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014060982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6607670.1"
                     /translation="MDEQRDAARQSETAGAEVSGIAAELAAPEEQLEDTRWVLILGAS
                     SGFGAAVAVALAEAGFNICGVHLDMRARMAGVEEVIRRIEATGRQVAFFNVNAADPEK
                     RKQVLDSMEQRMQGDGFRVLLHSLAFGTLQPFVSPAPGQTLINQKQVDMTLDVMAHSL
                     VYWTQDLVARNLLRRGSRILAMTSAGGSRVIPNYGAVSAAKAALESHIRQLAMELAPR
                     GITANAILAGVTDTPALRRIPGNDKIVAGALARNPSGRLTTPVDIARFIAMWAGPGSQ
                     WATGNVLQVDGGEEIVG"
     gene            complement(3811..4095)
                     /locus_tag="VF276_12220"
     CDS             complement(3811..4095)
                     /locus_tag="VF276_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607671.1"
                     /translation="MAWIHVRHRVQNYNKWKEIYDKTAGYKRHEGWKRYRVYQVSGDR
                     EDLIVMEQFSTLEQARAYAGSQFLRDAFEQEGVVGQPEILLLDGLEEGTA"
     gene            complement(4116..>4668)
                     /locus_tag="VF276_12225"
     CDS             complement(4116..>4668)
                     /locus_tag="VF276_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607672.1"
                     /translation="RMVGRAAPLLVALDGRSGTGKSTLAALLAARLGATVVEGDDFYP
                     GGSDAEWDARSPADKAALVIDWRRMRAEALAPLLAGRPAAWHPFDFQAGAGLAAHTVT
                     RAPAPVIVLDGVYSARPELADLVDLAVLVEAADDRVRRQRLLAREGAPYMAAWHRRWD
                     AAEDYYFTHIRPRSSFDRIITSV"
BASE COUNT          781 a         1547 c         1624 g          716 t
ORIGIN      
        1 cgccgactgg aaggccacga accgcccgtc ggcgctgatc gcggcggcgc tgctggggcc
       61 ggtggctgtg ccggtcaggg gcacgctggc ccagatcgtg gtgcccagca ggcgatcccg
      121 gacatagaca tcgggcaggc cgttggtgtc ggtgggcacc agggccgcca cggactcgaa
      181 cgcgacaaag cggccatcgc cgctaatggc cgggccgctg ctcatgtccg gcgcctgggc
      241 gccgttcgcc gcgaggctca cccgctcggt ggcgggcgcg ggcaggggcc gggcgacggg
      301 ggccggaggc gattcggcgg gggcggagcg caggcttgcg gtcagcaaca ggacgggcag
      361 cgtgaagcca aacaaagcga gacggcgcgg aggcatcagg gtcctttccg gcaaagtgaa
      421 aggctccgca aacaactgcc ggagccttgg caaatatcag cagcgggcgg cgggcccgcc
      481 ggggcttact gtgtgagtag cttgttgagc ttgctttcca tgctggcgcg gtcgaggccg
      541 gtgaccacga ggtctctgat cacgccgtcg cgccccacga agatgtgggt gggaatcccg
      601 gtcacgtaat aggcgcggga tgccttgccg tccaggtcca gcgcaaactt ccagctatag
      661 ccgccctgct ggacgaactg cttcacctgc gcctgcgact cctgcacatc cacgcccagg
      721 atttcgatgc ccttggcctg gtaggcggga tagagcttct ccatctccgg catctcggcg
      781 cggcagggcg ggcaccaggt ggcccagaag ttgacccaga ctggtttgac gccgcggtag
      841 ctgctcaact gcacggtgcc accgtcgaga cccgtcaggg tgaagtcggg cgccagatag
      901 cccacgcgcg ggctttcctt gccgcccccg gccggcgtgc ccacgttgat cgtggacgag
      961 gcgcccgcgg cgccggctgc ggcgggcgtg gcggggctcc gcggggtgag catgatggcc
     1021 gccatgagca caatcaccac cactgcggcc accaccgaga tcacgatggg caccatcgag
     1081 ccgcgcgggg ccggggcctc ctcgtactca taggcatacg ggtcggtctc ggcgggttcg
     1141 gtatggatgc tgtcgtcgat ctcgggcgtc tctgccgcgg cacgcacccg gcggcggggc
     1201 aacgtcccat acgcgggggc atcgtccgtc ggcagatcct ccgcggcatc ggcaacagga
     1261 tagtcgggat tactgctcat aggccgttcg cgcccggccc ccggaaccgg gagatcgagg
     1321 cgctgctcct ttcaccatta catcggaccc ctgcgggcgg aaaacgtgct gctgattcca
     1381 ttacggcgca agggccgccg tgggatttac gccagactgc ctactggctc atcaatttcg
     1441 tcacggcggc gtccatctgc tcgcgggtaa tgctgcccac gacgatgttc tggatcacgc
     1501 cgtcgcgccc gataaagaca tgcgtcggca ggccggtgat gcgatactgg cgcgtgatct
     1561 tgccgtcgag atcgagcagg aaagaccagg tatagccgcc ctgggtgatg tacgggcgca
     1621 ccaactgggc ggactcctga tcgtccacgc cgaggatctc cagaccccgc gcctggtact
     1681 gtttgtagaa ctgctgcatc tcgggcatct cgaccgcgca atgcgggcac caggtggccc
     1741 agaagttgac ccacaccggt ttgacgccgc gatagctgct gagctggacg ttgccgccct
     1801 ccaaccgttg caggctgaag ttgggcgcca gcgacccgat gctcatggct tcgggcaccc
     1861 cggcggtggc cggcttgctt ggggtcagaa tgatgctgac cagcaacccc agcaccagca
     1921 ggccgctggc ggcggcgaca atgatcgaga tccgcgcggc gtgcgccggg ttggcggcag
     1981 cggaatacgg ctccgggcta tccagggaag cggaatacgg ctctgagcta tcgagggaag
     2041 tcgtttcgtc actcatggtg attccatcct tgccagatac gccgggcagc gcaggccact
     2101 gctactatag ggatgattat actacacccg ccggggtgcg cacaaatccc cggcggggtt
     2161 cgcggcaggg agaggcgggg attcgcgggg agtgcagagg attgaagaca aatgcgccat
     2221 caggattcag atgccggatc ttcaagcttg tcggcagcga gcgcatccaa cagccgctgg
     2281 gcagcggcat ccaggcccga tggcgtgtag atcacatagc tgggatcggg gggcggagcg
     2341 ccgcgttgca gccaatccac cagcaccgca tcgacggatc gattggtcaa ggccgcctcg
     2401 gcgcgcgctc gttgcagcag cgcgtcggga accgccagcg tcacggaatt gggcattggt
     2461 gcatacctcc tgctaacggg atcgtagcac agggcgtgcc gaaaggcaag caaaccggcg
     2521 cagagtcggc tgggtgggga gacgcaaaag ttgcgccggg cgcggggcgg ggctataata
     2581 gcgaggctgc ccgcaagccg ggcggctgcg gaccaacagg ccaacacaag gaggcagagg
     2641 cgtatggatg agcagcgcga cgcggcccgg cagagtgaga cggcgggcgc cgaggtgagc
     2701 ggcattgcgg ccgaactggc ggcgccggag gaacaattgg aagacacccg ctgggtgctc
     2761 atcctgggcg cgtcgagcgg cttcggcgcg gcggtggccg tggccctggc cgaggccggg
     2821 ttcaacatct gcggcgtgca cctcgatatg cgcgcgcgca tggcgggcgt cgaagaggtg
     2881 atccgccgca tcgaggccac cggtcgccag gttgcgttct tcaacgtcaa cgccgccgac
     2941 ccggagaagc gcaagcaggt cctcgactcc atggaacagc ggatgcaggg cgacggcttc
     3001 cgcgtgctgc tacactccct ggccttcggc accttgcagc cgtttgtcag cccggcgccg
     3061 ggccagacgc tgatcaacca gaagcaggtc gatatgacgc tcgacgtgat ggcccacagc
     3121 ctggtctatt ggacgcagga cctggtcgcg cgcaacctgc tgcggcgcgg cagccgcatc
     3181 ctggcgatga ccagcgcagg cggcagccgg gtcatcccca actacggcgc cgtctcggcg
     3241 gccaaggccg cgctggagtc gcatatccgc caactggcga tggaactggc cccgcgcggc
     3301 atcaccgcca acgccatcct ggccggcgtg accgatacgc cggccctgcg ccgcatcccc
     3361 ggcaacgaca agatcgtggc gggggcgctg gcccgcaacc ccagcgggcg gctgaccacg
     3421 ccggtcgata tcgcccgctt catcgccatg tgggcgggcc ccggttcgca gtgggccacc
     3481 ggcaacgtgc tccaggtgga cggcggcgag gaaatcgtcg gctagatcgg cgcgggccgg
     3541 tttgaaaccg gcccctacac cgcctgccgg tcagcatggt gccctggcag ccgtgggccg
     3601 gtttgaaacc ggcccccaca ccgccgccgg gacagagcgt tgggtaggca ggtcggtttg
     3661 aaaccggccc ccacaccgcc gccgggccgg gatggccctc ggtagggtac cacccccggc
     3721 ttgtcattct gagcgcagcg aagaatctcc gtcttatagg cgcagcgacg agactcttcg
     3781 ctgcgctcag agtgacaatt gtggtgttac tcaggcggtg ccctcttcca gaccatcgag
     3841 cagcaggatc tcgggttgcc cgacgacgcc ttcctgctcg aaggcgtcgc gcaggaactg
     3901 cgaaccggca taggcgcgcg cctgctccag cgtggagaac tgctccatga cgatcagatc
     3961 ctcgcggtcg cccgagacct ggtagacgcg atagcgcttc cagccctcat ggcgcttgta
     4021 cccggcggtc ttatcgtaga tctctttcca cttgttgtag ttctgcaccc ggtggcgcac
     4081 atgaatccag gccatgatgc ttgctccttg ctccttcata cggaagtgat gatccggtcg
     4141 aacgacgacc ggggccggat gtgggtgaag taatagtcct cggcggcgtc ccagcgccgg
     4201 tgccaggcgg ccatgtaggg cgcgccctcg cgggccagca gccgctgccg ccgcacacgg
     4261 tcgtcggcgg cctcgaccag gaccgccaga tccaccagat cggccagttc cgggcgggcg
     4321 gaatagacgc cgtcgagcac gatgaccggc gccggggcgc gcgtgacggt gtgcgcggcc
     4381 agccccgccc ccgcctggaa gtcaaacgga tgccacgcgg cggggcgccc ggccagcagc
     4441 ggcgccagcg cctcggcgcg catccgccgc cagtcgatga cgagcgccgc cttgtccgcg
     4501 ggcgaccgcg cgtcccactc ggcgtcgctg ccgcccggat agaagtcgtc gccttccacc
     4561 accgtcgcgc ccaatcgggc cgccagcagc gcggccaggg tggacttgcc cgtgccgctg
     4621 cgcccgtcca gggccaccag cagcggcgcc gcgcgcccga ccatccgg
//
LOCUS       DASUYC010000743         2860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_319364,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000743 DASUYC010000000
VERSION     DASUYC010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319364"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1326)
                     /locus_tag="VF276_12230"
     CDS             complement(<1..1326)
                     /locus_tag="VF276_12230"
                     /inference="COORDINATES: protein motif:HMM:NF023650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan binding domain-containing protein"
                     /protein_id="HEX6607673.1"
                     /translation="MSYQPDDRTPRDLGQPAEPASKEAPAASKEAAAPPDAQRSGYIP
                     RVPRPATSQVAPPRPPAANEAPAARPTPPPGDIPAWEDRPTAPMPVMNQTAPHVQPAP
                     RPTPPPARDYPTAPPAHNYPPAPARDHQAAPADWAQQQAAAPVHTHPAPAPRPAAPRA
                     NPPAAAPPAPPVYDPARAHVAPAPARSRGRGGMFLGVLAGLIALAAIGVFALAGWYDQ
                     QYAGHLFPGVRVLGVDIGGKTPDEARVMLENKVAAFTDQPIVLVWNDQQWTPQPKQLG
                     MQVNLDRTLDNAYNVGRGDGLLNTWRERWDVANEGRVVPLSVSLDEAALQAYLQNEVA
                     PKIDQDLQEGDVWLQGEQVNTTESREGRKLEVYNALVAIRESLARMTTNRIDLPVTIT
                     KPAVAQSEIDQTATALKTMLGGPLVAHYGSKQFSVAPRDISQKLLLLGRN"
     gene            complement(1498..2082)
                     /locus_tag="VF276_12235"
     CDS             complement(1498..2082)
                     /locus_tag="VF276_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607674.1"
                     /translation="MARARGIGRVAQALLVGIAGLALLVPAAGAAGAQVRILEPAAGA
                     TVNAASVPVALAVEGADLPADAARWPGPGVFHVALDGVDVLQTQELRFTLLAIPPGAH
                     RLRVTLEGANPPPAPTEIAFSAQVVAPPAGASWLGAGGVAVAAAVMLVALLLLWLVWV
                     RPQRIEALPEAPPAAGEAPAAPDEPVELDPPPAR"
     gene            complement(2101..2562)
                     /locus_tag="VF276_12240"
     CDS             complement(2101..2562)
                     /locus_tag="VF276_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX6607675.1"
                     /translation="MSGLTNLPFDIVILLLRLAFVFLLYFFVFQVVRTLTRDLRGVKP
                     VEAPASPYGRLIVVNPGSTGIAPGTGFNLEPITSIGRRLTNTIVLDDNFISGEHSLLT
                     WRDGHWWAEDYRSTNGTLLNGAELTRPTAMRDGDVLTIGGVQLKLARPAGA"
     gene            complement(2559..>2860)
                     /locus_tag="VF276_12245"
     CDS             complement(2559..>2860)
                     /locus_tag="VF276_12245"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX6607676.1"
                     /translation="ARPAQPPASLHLISGAAGTVVQITSPLTSLGRELDNDLVIEDSR
                     VSRHHAQILYQHNRYAVRDLGSTNHTFVNGRQVETSVLAPGDRISLGGFELEFML"
BASE COUNT          387 a          948 c         1081 g          444 t
ORIGIN      
        1 gttgcgcccc agcaggagca gcttctgcga gatgtcgcgc ggggcgaccg agaactgctt
       61 ggacccgtag tgggccacca gcgggccgcc gagcatcgtc ttgagcgcgg tcgcggtctg
      121 gtcgatctcg ctttgcgcca ccgcgggctt ggtgatggtc acggggagat cgatgcggtt
      181 ggtggtcatg cgcgccaggc tctcgcggat ggcgaccagc gcgttataga cttcgagctt
      241 gcgcccctcg cgcgactcgg tcgtgttcac ctgctcgccc tgcaaccaga catcgccttc
      301 ttgcaggtcc tggtcgattt tgggcgcgac ctcgttttgc agataggctt gcaaggccgc
      361 ctcgtccagg ctgaccgaga gcggcaccac gcggccctcg ttcgccacgt cccagcgctc
      421 gcgccaggtg ttcagcaggc cgtcgccgcg ccccacgtta taggcgttgt cgagcgtgcg
      481 gtcgagattg acttgcatgc ccagttgctt gggctgcggg gtccactgct ggtcgttcca
      541 gaccagcaca atcggctggt cggtgaaggc ggcgactttg ttttcgagca tgacgcgcgc
      601 ttcgtcgggc gttttgccgc cgatatcgac gccgagcacc cgcacgccgg ggaacaggtg
      661 cccggcatac tgctgatcgt accacccggc cagggcgaag acgccgatgg ccgccagggc
      721 gatcagcccg gccaggacgc ccaggaacat gccgccccgg ccacgcgacc gggcgggagc
      781 gggggccaca tgggcgcggg ccggatcgta gaccggcggc gcgggcgggg cagcggcggg
      841 cgggttggcc cgcggcgcgg cgggccgggg tgcgggcgcc gggtgcgtgt gaaccggcgc
      901 ggcggcttgc tgctgcgccc aatcggcggg cgcggcctgg tggtcgcggg ccggcgcggg
      961 cggatagttg tgagcaggcg gcgcggtcgg gtagtcgcgg gcgggcggcg gtgtcgggcg
     1021 gggtgcaggc tgcacatgtg gcgcagtctg gttcatcacc ggcatcggcg cggtgggccg
     1081 gtcttcccag gccgggatgt cgccgggcgg gggggtgggc cgggccgccg gggcctcgtt
     1141 ggcggcgggc gggcgcggcg gggcgacctg tgaggtcgcc gggcggggca cgcgcgggat
     1201 gtagcccgaa cgctgggcat cgggcggcgc ggctgcctct ttcgaggcag ccggtgcctc
     1261 tttcgaggca ggttccgccg gctggcccag gtcgcgcggt gtgcggtcgt caggttggta
     1321 gctcataaca aagtctcttc ccgcggtgcg ctgcgccccg tccaccaggc cggcagccta
     1381 cgaatctgcg cttcggggcc gggctcacgg cgcaaaccgg cccgcgagtc tgcgaatctg
     1441 ggtctattgt aacatatccc ggcccaccac ggcgcaattt ccgtcaacat cattatgtta
     1501 acgggcgggc ggcggatcca gttctaccgg ctcatccggc gcggcgggtg cctctcccgc
     1561 ggcaggcggc gcctcgggca gtgcctcgat ccgttgcggg cgcacccaga ccagccagag
     1621 cagcagtagg gcgaccagca tgacggcggc ggccacggcc acgccccccg cgcccagcca
     1681 gctcgcgccc gcgggcggcg ctaccacctg ggcgctaaat gcgatctccg tcggggcggg
     1741 cggcgggttg gccccttcga gcgtcacgcg caggcggtgg gcgccgggcg ggatcgccag
     1801 cagcgtgaag cgcaactcct gggtttgcag cacgtccacg ccgtccaacg ccacgtggaa
     1861 cacgcccggc cccggccagc gggcggcatc cgccggcagg tccgcgccct cgactgccag
     1921 cgccaccggc acgctggccg cgttcacggt cgcccccgcc gcaggctcca ggatgcgcac
     1981 ctgtgcgccc gcggccccgg cggcgggtac cagcagcgcc agccccgcta tgcctacgag
     2041 caaagcctgg gccacgcgcc cgatgcctcg ggcgcgtgcc actcgccagg ggtgcacggg
     2101 ctaggcgccc gccgggcggg ccagcttgag ttgcacgccg ccgatggtca gcacgtcgcc
     2161 gtcgcgcatg gccgtgggcc gggtcagttc cgcgccgttg agcaacgtgc cgttcgtgct
     2221 gcggtagtcc tcggcccacc agtggccgtc gcgccaggtg agcaggctgt gttcgccgga
     2281 gataaagttg tcgtcaagga cgatggtgtt ggtcagccgg cggccgatgc tggtgatcgg
     2341 ttccaggtta aagccggtgc cgggggcgat gccggtgctg ccggggttga ccacaatgag
     2401 gcgcccatag gggctggccg gcgcttcgac cggcttcacg ccgcgcagat cgcgcgtgag
     2461 cgtgcgcacc acctggaaca cgaagaagta gagcaggaac acgaacgcca gccgcaacag
     2521 gagaatgacg atatcgaagg gtaggttggt tagtccactc ataacataaa ctctaactca
     2581 aaaccgccca gcgagatacg gtcgccgggc gccagcacgc tggtctcgac ctgccggccg
     2641 ttgacgaagg tatggttggt gctgcccagg tcgcgcaccg cgtagcggtt gtgctggtag
     2701 aggatctgcg cgtggtggcg cgagacccgg ctgtcttcga tgaccagatc gttgtcgagt
     2761 tcgcgcccca gcgaggtgag cgggctggtg atctggacca cggtgcccgc cgcgccgctg
     2821 atcaggtgca ggctggcggg gggctgggcc gggcgcgcgc
//
LOCUS       DASUYC010000744         2522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1730746, whole genome shotgun sequence.
ACCESSION   DASUYC010000744 DASUYC010000000
VERSION     DASUYC010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1730746"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VF276_12250"
     CDS             <1..258
                     /locus_tag="VF276_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015821558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEX6607677.1"
                     /translation="YAPRNVLIIDPVLPAWLPDLTVRDLRIGAGRVSIRFRRGRDGET
                     GYRVLERHGPVHVLRQPPPDDLHAGPFARLRDLAGSLLPSS"
     gene            754..>2522
                     /locus_tag="VF276_12255"
     CDS             754..>2522
                     /locus_tag="VF276_12255"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEX6607678.1"
                     /translation="MSNIPRAGILSGALLLATVLFAPARAAQPATPAVSPLLTDPMSI
                     SVDARVSAAEVQALHQAGVQLVRYPLAWWDVQPQDTGPGQYNWSIPDAGLQALAAANV
                     DVLLVLGGNPRWAASKTDGPVNRVPVARYYDFVTQAATRYGRSPYTVRAWEIYNEEDS
                     TQTGWGTNAAGYAALLHQDYLIIKAIAPQTDVVMGGLAYDWFQDQCQPNGTCGPFVRN
                     FLPNFLAAGGGAWTDAVNFHHYPRTFWPSLGAVVQALQTTLQAAHVQKPLLWTETSSP
                     SSPEYQGSLEAQSAYVVQAYASGLGQGLNAITWFPLQDFTSTTFRYFTTDGLMNLDNS
                     YKPSFLAYQVAAGYLGRALFLRPLTAQELVGTTVGEGYLFLETDGNGVTVAWTRQGTG
                     SESLLAAHVRAVKNKYGRAVAYTVSNGQAIIPLSTDPVYAELQLVSPSATATNGPPAT
                     ATAQGTATPTPLTPQPPSDTATATPSPPATASATPSPCLSGFSDVQPSDYFYTPVRYL
                     VCRGAISGYADGTFRPANPTSRAQMVKIVVLGFSKPVTTPPAGGYTFADVPPSHPFFA
                     FIETAAATGIVSGYTCGGPGEPC"
BASE COUNT          398 a          897 c          792 g          435 t
ORIGIN      
        1 tatgcacccc gcaacgtgct gatcatcgac ccggtgctcc cggcgtggct gcccgatctg
       61 acggtgcgcg atctgcgcat cggggcgggc cgcgtctcga tccgcttccg gcgcgggcgc
      121 gacggcgaga ccggctatcg ggtgttggag cgccacggcc ccgtccacgt cctgcgccag
      181 ccaccccccg acgacctgca cgcggggcct tttgcgcggc tgcgggacct cgcgggatcg
      241 ctgctgccct catcctgaac acttgcgcgg tctgccggga ttgacaggcc cggattatga
      301 tgctagtatt atccattgaa cggggctacg tatcgtagtg agctcagggt catgccgcgc
      361 tacccctagc cgaactgagg atccttggtg gtccggaatc cttgctagag tggtttaccg
      421 acggctctcg ccgatctgtc tgcttgtcgc cttctacgcc cgaggcgcct tgcacgagga
      481 accggcgcta agcgaccact caattcccat tccggtgttc cggggcagcg cgtccttgcc
      541 tcgcgccacg ctccgccccg catgtcccgc cgccggtcta ccccgtgccg gcaagctgct
      601 gccgcctaca gcgaaaccgc gggttgccca cccctccagt tcgatccatt cccgtgccgg
      661 cgggaatgcc ctggttcgcg actgatctgc tagaagcgcc gcatgctgcc ccatggcgat
      721 gatacccgcc cggcgcgaga ggcgaaaaga ggcatgagca acataccgcg cgcgggcatc
      781 ctctccggcg cactcctgct ggcgacggtg ctgttcgctc cggcacgtgc ggcgcagccg
      841 gcgacccctg ccgtctctcc gctactgacg gatcccatga gtatcagcgt cgacgcccgc
      901 gtgagcgctg cggaggtgca ggcgttgcac caggccggcg tccagttggt ccgctatccg
      961 ctcgcctggt gggatgtcca gccccaagac acgggccctg gccagtataa ctggagcatc
     1021 cccgatgcgg gcctgcaagc gctggccgcc gccaatgtgg atgttctgct ggtgctgggc
     1081 ggcaatccgc gttgggcggc ctcgaaaacg gatggccccg tcaatcgcgt ccccgtggcg
     1141 cggtattatg atttcgtaac ccaggccgcc acccgctacg gccggtcccc gtatacggtg
     1201 cgcgcctggg agatctataa cgaagaggac tccacgcaaa cgggctgggg caccaacgcg
     1261 gccggctacg cagcgctctt gcaccaggac tacctgatca tcaaagccat cgcgccgcaa
     1321 accgacgtgg tgatgggcgg gctggcctat gattggttcc aggatcagtg ccagcccaac
     1381 ggcacctgcg gtccctttgt ccgtaatttc ttgcccaatt tcctggccgc cggcgggggc
     1441 gcctggaccg atgccgtgaa cttccaccac tatccgcgca cgttttggcc gagcctgggc
     1501 gcggtggtgc aggcgctgca gacgacgctg caagccgccc atgtgcagaa accgctgctg
     1561 tggacggaaa ccagctcgcc cagcagtccc gaataccagg gcagcctcga agcgcagtcg
     1621 gcctacgttg tgcaggcgta tgctagcggg ctgggtcagg gtcttaacgc catcacctgg
     1681 ttcccgttgc aggatttcac cagcacgacc ttccggtact tcacgacgga cggcctgatg
     1741 aacctcgaca actcatacaa gccctcgttt ctggcctacc aggtcgccgc aggctacctg
     1801 gggcgcgcgc tgttcctgcg gcccttgacc gcgcaggagc tcgtgggcac gaccgtgggc
     1861 gaaggctacc tgttcctgga gaccgacggc aatggcgtca cggtcgcctg gacccgccag
     1921 ggcaccggca gcgagtcctt actggcggcc cacgtccgcg cggttaagaa caagtacggc
     1981 cgggccgtgg cctacaccgt gagcaacggt caggcgatca tcccgctgag caccgatccg
     2041 gtgtatgccg agttgcaact ggtcagcccg agcgccaccg caacaaacgg cccccccgcc
     2101 acggcgacgg cgcaggggac ggccaccccg acgccgctga ccccgcaacc gccgtcggat
     2161 acggcgaccg cgaccccgtc gccaccggcg actgcttctg ccacgccgtc cccctgcctg
     2221 tccggtttta gcgatgtgca gccgagcgac tacttctata cgccggtgcg ttatctggtc
     2281 tgccggggcg ccatcagcgg ctacgccgac ggcaccttcc gcccggcgaa cccgacgtca
     2341 cgcgcccaga tggtcaagat cgtggtgctg gggttcagta aacccgtgac gaccccgccg
     2401 gcgggcggtt acacgtttgc cgatgtgccg cccagccacc cgttcttcgc cttcatcgag
     2461 acggcggcgg ccaccggcat cgtcagcggc tacacctgcg gcgggccggg cgaaccgtgc
     2521 ga
//
LOCUS       DASUYC010000745         3299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_600453,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000745 DASUYC010000000
VERSION     DASUYC010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600453"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VF276_12260"
     CDS             complement(<1..428)
                     /locus_tag="VF276_12260"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XamI family restriction endonuclease"
                     /protein_id="HEX6607679.1"
                     /translation="MQEPLAAYLAAFAAVEVAVDGLFQATDDLARLDQNAALLLLNPD
                     FVEVLRYLAAPPISLDDLLTLANVPSLGPTAVLRDKDLTRRVLETIRAGLDPRRFPWA
                     GAGRAPAPAERAAAVLATTAIIATRRVETMRRTEGKNEQEA"
     gene            complement(530..2185)
                     /locus_tag="VF276_12265"
     CDS             complement(530..2185)
                     /locus_tag="VF276_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006897784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEX6607680.1"
                     /translation="MLKQISFTNVLDPAAAPVVPSMPDLLAVAFALGARSVAGWSPNE
                     EDLAATLPAPDPASVAGAQHAIQHGADPLGDAYCRLLSAEQRRPMGATYTPRAIVAAM
                     LQWAATQVCPARVVDAGTGSARFLVGAGRMFPQAALCGVESDPRAALLARGHLAAAGF
                     APRAYVAVGDYRAAALPAIAGPTLFVGNPPYVRHHLLEPRWKAWLVQTARKYGLAASQ
                     LAGLHVHFFLATAEYARPGDIGVLITAAEWLDVNYGRFVRELLLDVLGVKTIHIIEPA
                     ALPFPDAAATAVITGFAVGARPKAVGVRRVAHLDELGALSSVVQVPRERLEAAGRWTL
                     LTHAVPEQRAGFVELGELCRVHRGQVTGANQVWIEGRHSADLPESVLFRTVTKARELF
                     QVQDVLADASHLRRVIDLPVDLDIFAPEDRRRIERFLAAARAQGADSGFIARHRKAWW
                     AVGLRDPAPILATYMARRPPAFVRNLAGARHINIAHGLYPRVPLAEPALRALALYLSN
                     HTLLGQGRTYAGGLTKFEPREMERLLVPGPELLLTGHWAEVPA"
     gene            complement(2366..2773)
                     /locus_tag="VF276_12270"
     CDS             complement(2366..2773)
                     /locus_tag="VF276_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HEX6607681.1"
                     /translation="MPARSSFDYAIVRVVPRVERGEFINAGVILFCRTRRFLEARIEL
                     DTARLLALAPDVALAEVREHLAVIPRVAAGGPDAGPIGQLSQAERFHWLVAPRSTIIQ
                     VSPPHSGLCTDPAAELENLLNTMVRTQPGVPAG"
     gene            complement(2751..>3299)
                     /locus_tag="VF276_12275"
     CDS             complement(2751..>3299)
                     /locus_tag="VF276_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HEX6607682.1"
                     /translation="EPDPDIQALLKASTGLNLALDYLPGSLAFDPAAAKTVDPALASA
                     IVWFDAYVTNVDRTARNTNMLLWHKLLWLIDHGAALYFQHTWADYLARSRTPFAAIKQ
                     HVLLPYAADLPTADAALRPRLTPGVLEPILAAIPDAWLDVGPGLGSPAATRAGFLEYL
                     LARLEAADVFVEEAIHARAQLV"
BASE COUNT          571 a         1122 c         1126 g          480 t
ORIGIN      
        1 gcttcctgct catttttacc ctcggtgcgg cgcatggttt cgacccggcg ggtggcgatt
       61 atggctgtcg tagcgagcac ggcggcggcc cgttccgccg gagccggcgc tcgccccgcg
      121 ccggcccacg ggaaccggcg cggatctaac cccgcgcgga tcgtctccag cacccgccgg
      181 gtcaggtcct tgtcacgcag cacggcggtg gggccaagcg aggggacgtt cgccagcgtc
      241 agtagatcgt ccagcgaaat cggcggcgcg gccagatagc gcaagacttc gacaaaatcc
      301 ggattgagca gcagcaaggc ggcattttga tcgagccggg cgaggtcatc cgttgcctga
      361 aacaggccgt ccaccgcgac ctccaccgcg gcaaaggcgg cgagataagc agcaagcggt
      421 tcttgcatgc gcgcctggcg aaatacatcc tccgcggttt tacgatccgc ctccagttct
      481 tccggcgtcc acattcttgg cgggcggaag ggggatcgcg gccggttggt catgccggca
      541 cctcggccca gtggccggtc agcagtaact cgggaccggg cactagtaag cgctccatct
      601 cgcgcggctc gaacttcgtc aggccgccgg catacgtccg gccctgcccc aacagggtat
      661 ggttggacag gtagagcgcg agcgcgcgca aggccggctc cgctaacggc acacgggggt
      721 agagaccgtg cgcgatattg atgtggcggg caccggccag gttgcgcaca aacgccggtg
      781 ggcggcgggc catatacgtg gcgagaatgg gcgccgggtc gcgcaaaccc acggcccacc
      841 acgctttgcg gtggcgggca atgaatccgc tatccgcgcc ctgcgccctg gccgccgcca
      901 ggaagcgctc aatccgccgg cggtcctccg gcgcaaatat atcaagatcc accggcagat
      961 cgatgacccg gcggaggtgc gaggcatcgg ccagcacatc ctgcacctgg aataattctc
     1021 gtgctttcgt cacggtacga aacaacacgg attcgggtaa atcggcgctg tgccggcctt
     1081 caatccagac ctggttggcg ccggtaactt gcccgcgatg gacccggcat aactccccca
     1141 gttcgacaaa gcccgcgcgc tgttccggca cggcatgcgt gagcagagtc cagcgccccg
     1201 ccgcctccag ccgttcgcgg gggacctgca ccacactgct caacgcaccc aattcgtcca
     1261 ggtgcgccac ccggcgcacg ccaaccgctt tgggccgcgc gcccactgca aaacccgtaa
     1321 tcaccgccgt cgccgcggca tcaggaaaag gcaacgcggc cggttcgatg atatggatcg
     1381 tcttcacgcc caggacatct agcagcagtt cccgcacgaa ccggccatag ttgacatcca
     1441 gccattcggc ggcggtaatc aacacgccta tatcgccggg ccgggcgtat tccgccgtgg
     1501 cgagaaagaa atggacgtgg agaccggcga gttggctggc ggccagacca tatttgcgcg
     1561 ccgtttggac cagccaggct ttccagcggg gttccagcag gtgatggcgc acatacggcg
     1621 ggttgccgac aaagagagtg ggaccggcaa tggcgggtag tgccgccgcc cgataatccc
     1681 cgacagctac atacgcgcgg ggcgcgaagc cggccgccgc gaggtggccg cgcgccagca
     1741 gcgcagcccg cggatcagac tccacgccgc aaagcgcggc ctgcgggaac atgcggcctg
     1801 ccccgaccag aaaacgcgcc gatccggtgc cggcatccac cacccgcgcc gggcagacct
     1861 gggtcgcggc ccattgcagc atggccgcga caatggcccg gggcgtgtac gtcgctccca
     1921 tgggccgccg ctgttcggcg gacagcagcc ggcagtaggc atcgcccagc ggatcagcgc
     1981 catgctgaat cgcgtgctgc gccccggcca ccgatgccgg gtcgggggcg ggcagggtcg
     2041 cggctagatc ctcctcattc ggcgaccacc cggcaacgct ccgcgcgccc aacgcaaacg
     2101 ccaccgccag tagatcgggc atcgatggga ccaccggcgc tgccgccggg tctaggacgt
     2161 tcgtgaatga aatctgcttc agcatcaatt acctcgcctg agcaaacgga ccggttgccg
     2221 gtggacggtg gtcacggcta tttggccgca gtatcgcaaa ctcagcatga aaaagcaagc
     2281 ggctgaacgg ccttcgcccg gccatccggt gcccttactc ctgtatcggg cggcgctgcc
     2341 gcaaatcgga tggccgccgc gccgctcagc ccgcgggcac gcccggctgg gtgcgcacca
     2401 tcgtgttgag caggttctcc agttcggcgg cggggtcggt gcagaggccc gagtgcggcg
     2461 gcgagacctg gatgatcgtg ctgcgcgggg ccaccagcca gtggaagcgc tcggcctggc
     2521 tcagttggcc gatggggccg gcgtccgggc cgcccgccgc cacgcgcggg atcacggcga
     2581 gatgctcccg cacttcggcc agggccacgt cgggcgccag ggccagcagg cgcgccgtgt
     2641 ccagctcgat acgcgcttcc aggaagcggc gggtgcggca gaagaggatc acgcccgcgt
     2701 tgatgaactc gccgcgctcc acccgcggca ccacgcggac gatggcgtag tcaaacgagc
     2761 tgcgcgcggg catggatcgc ctcctccacg aacacgtccg ccgcctccag gcgggccagc
     2821 aggtactcca gaaaccccgc gcgcgtggcg gcggggctgc ccagccccgg cccaacgtcc
     2881 agccaggcat cggggatcgc ggcgagaatc ggctccagca cgccgggcgt gagccgcggg
     2941 cgcagtgcgg cgtcggcggt gggcagatcg gcagcgtagg gcaacagcac gtgctgcttg
     3001 atggcggcga acggagtgcg gctgcgggcc aggtagtcgg cccaggtgtg ctggaaatag
     3061 agcgccgcgc cgtggtcgat cagccagagc agtttgtgcc agagcagcat gttggtattg
     3121 cgcgcggtgc ggtccacgtt cgtgacatag gcgtcgaacc agacgatggc cgaggccagg
     3181 gccgggtcca ccgtcttggc tgccgccgga tcgaaggcca gcgaccccgg caggtaatcc
     3241 agggccaggt tcagcccggt gctcgccttg agcagcgcct ggatatccgg gtccggctc
//
LOCUS       DASUYC010000746         2165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1621388, whole genome shotgun sequence.
ACCESSION   DASUYC010000746 DASUYC010000000
VERSION     DASUYC010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1621388"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VF276_12280"
     CDS             <1..272
                     /locus_tag="VF276_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607683.1"
                     /translation="PRTPPTVRYDAEIFRILGIAAPSASHHIVSGSTMCLYGWDQWRA
                     VTMRVVLQQRVVNHVASLLKIGDGQAPVAAFAGKQHIYNDRDDWR"
     gene            666..1583
                     /locus_tag="VF276_12285"
     CDS             666..1583
                     /locus_tag="VF276_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607684.1"
                     /translation="MTNPVVLMSASAYAAACAEFARWAAHGRRMGGAAWEAVVYPLVG
                     LVARPGMARSPLEATPPAACAALVVPRVVLPPPELAHYGATWARPAAVSNLAAATALQ
                     ARLDAVRAQHPRLDCYGRMHSHPWPHRQPRPSGTDCAEHLTGALDANRAAGLPWALGL
                     IAAAPGAGSAEWPIAAYALGAGGTITDLGPVRVVRDADPRVRQVLARPGWATPTGGRR
                     LERQVAALTARGVAVTALPLHRGWYGLVMRPPGRPACLLALPPGFPTAAPRLLRPAAG
                     GWTGAAVRAARPRRDAYDWRSYPLVQEVP"
     gene            1583..>2165
                     /locus_tag="VF276_12290"
     CDS             1583..>2165
                     /locus_tag="VF276_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607685.1"
                     /translation="MASSPTYEEQPTLTTNEHGAINRAATLGGSREAPLLSRSADDRQ
                     AAYYARIVPVLGAGLRHARIAVCGVAAVAGAVERLAGCRAVRWWIADDAPLTPAHPLV
                     RGSDAAAGASAGPALAAGLQAHNAWETGWEFHHYPALTPATQATLAAAWRAAPPDLLL
                     GGGDAATLAALAALARDLAVPALLVAHYAGADPS"
BASE COUNT          250 a          820 c          805 g          290 t
ORIGIN      
        1 ggccgcgcac gcccccgaca gtgcgctacg acgcggagat cttccgcatc ctgggcatcg
       61 ccgcgccaag cgccagccac catatcgtct ccggcagcac gatgtgcctc tacggctggg
      121 accagtggcg cgcggtgacg atgcgcgtgg tgctgcaaca gcgcgtggtc aaccacgtcg
      181 ccagcctgct caagatcggc gacgggcagg ccccggtcgc ggccttcgcc gggaagcaac
      241 atatctataa cgaccgggac gattggcgct aggcggcgcg ggccggtttg aaaccggccc
      301 ctacaccgcg cccggtcctg gccggtggtg ccaaccagcg cgggccggtt tcaaaccggc
      361 ccctacaccg caccctggcc ctgtgcgcgg gtgccaacca gcgcgggccg gtttcaaacc
      421 ggcccctaca ccgcaccctg gccctgtgtg gcgggttgaa atcgtccgca cccggtcatg
      481 ccttcgctgt aggggtcggt ttcaaaccgg ccccgtacag gacacgcccg tggcgcgtat
      541 cccgccgctg taggggccgg tttcaaaccg gcccgtgccg cctacctcga tgcccgcccg
      601 acggcgctgt aggggccggt ttgaaaccgg cccgtgccgc cggaagaccc gagaaggaga
      661 ctgtcatgac gaaccccgtc gtgttgatga gcgcgagcgc gtacgcggcg gcctgcgccg
      721 agttcgcgcg ctgggccgcg cacgggcgcc ggatgggcgg cgcggcctgg gaggcggtgg
      781 tctatcccct ggtcgggctc gtcgcccggc ccggcatggc ccgcagtccc ctggaggcga
      841 cgccgccggc tgcgtgcgcg gcgctggtcg tcccacgggt ggtgctgccg ccgcccgaac
      901 tggcccatta cggcgccacc tgggcgcgcc ccgccgccgt gagtaacctt gccgccgcca
      961 ccgcgttgca ggcccggctc gacgccgtcc gcgcgcagca tccgcggctc gactgctacg
     1021 ggcggatgca ctcgcatccc tggccgcacc ggcagccgcg cccgtcgggc accgattgcg
     1081 ccgagcacct gaccggcgcg ctcgacgcga accgcgctgc cgggttgccc tgggcgctcg
     1141 gcctgatcgc cgccgcgccg ggtgccgggt cggcggagtg gcccatcgcg gcgtacgccc
     1201 tgggcgcggg cggaacgatc acggacctgg ggccggtgcg cgtggtgcgc gacgccgatc
     1261 cccgcgtgcg gcaggtgctg gcccggcccg gttgggccac ccccacgggc gggcgccggc
     1321 tggagcggca ggtcgccgcg ctcacggcac gcggggtggc ggtgacggcg ctgccgctgc
     1381 accgcggctg gtacgggttg gtcatgcgcc cgccggggcg cccggcctgc ctgctggcgt
     1441 tgccgcccgg cttccccacc gccgcgcccc ggttgctgcg gcccgctgcc ggcggctgga
     1501 ccggcgccgc cgtgcgggcc gcccgccctc gccgggatgc ctatgactgg cgttcctatc
     1561 cgctcgtgca ggaggtgccc tgatggcgag ttcaccaacg tatgaggaac agcccacgct
     1621 gacgaccaac gagcacggcg cgataaatcg cgcggctacg ctcggcgggt cacgggaagc
     1681 tccactgctg agcagatctg ccgatgaccg gcaagcggcg tactatgcgc gcattgtgcc
     1741 ggtgctgggg gccgggttgc ggcacgcgcg catcgccgtc tgcggcgtgg ccgcggtggc
     1801 gggcgccgtg gagcggctgg cgggctgccg cgccgtccgc tggtggatcg ccgacgacgc
     1861 gccgctgacg cccgcgcatc cgctggtgcg gggcagcgac gcggctgcgg gcgcatccgc
     1921 ggggccggcc ctggccgccg ggttgcaggc gcataacgcc tgggagaccg gctgggagtt
     1981 ccaccactac cccgcgctga ccccggccac gcaggcgacg ctggccgccg cgtggcgcgc
     2041 cgccccgccg gacctgctgc tgggcggggg cgatgccgcg acgctggcgg ccctggcggc
     2101 cctggcgcgc gatctcgccg tgcccgcgct gttggtagcc cactacgccg gcgccgaccc
     2161 cagcg
//
LOCUS       DASUYC010000747         3001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1268338, whole genome shotgun sequence.
ACCESSION   DASUYC010000747 DASUYC010000000
VERSION     DASUYC010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268338"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /locus_tag="VF276_12295"
     CDS             complement(<1..1011)
                     /locus_tag="VF276_12295"
                     /inference="COORDINATES: protein motif:HMM:NF024640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HEX6607686.1"
                     /translation="MNRNTHKGPRGAALVLGLGALLAAVVPAAPAARAAGAYDGAVQK
                     AASWVAGQQQATGAYPGFGVGSTADAVIALVAAGRSNPSSAGTKSALDYLAGQAATYA
                     KTPGAAAKLILAFDAATGATADMHQVGGVDLEQVITGGYDAATGQYGKDVTGHALSIL
                     ALTDLGRPLGAKALDWLRQSQGSEGGWAFDGSTGPGSADTNTTGLALQALAAAGAKSD
                     PVVPKALAYLHAQQNSDGGFAYQKGPDAASDSNSTGMVVIGLIAVGEDPAAWQQGGAG
                     PLDFLVRLQNASGALRYQAAQAEDNGGATYQAIPALAGLALPLPHTLANGTAPVIAPA
                     PGM"
     regulatory      complement(1334..1486)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2067..>3001
                     /locus_tag="VF276_12300"
     CDS             2067..>3001
                     /locus_tag="VF276_12300"
                     /inference="COORDINATES: protein motif:HMM:NF022629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HEX6607687.1"
                     /translation="MATVVGLFDNSTQAQGAIEQLRAAGIPSEHISLAMRDRSEAAAV
                     AEESEVGTGVATGAVGGGILGGLAGLLVGIGALAIPGIGPLVAAGPLAATLLGAGIGA
                     ATGGLIGALVGAGVPEEEAQLYQAGVERGGVLVTVDADPQQEAEVRDILDRSGMRDVR
                     ADAATWNENPEYRYGRRTDESVAPEAVGGAGGAVAGGVIGAAVGGPVGAAAGAAIGGA
                     AGAGAGHEVAEPETHPGGSEVGGAAGAVTGGVIGSVAGPVGAAAGAAIGGAVGGAAGE
                     GATDAASDTTTRPGYTTSDVTTDTTPGYTGSAPTYT"
BASE COUNT          429 a         1031 c         1073 g          468 t
ORIGIN      
        1 catgccgggc gcgggcgcga taaccggcgc cgtgccgttg gcgagcgtgt gcggcagcgg
       61 cagcgccagc ccggccagcg ccgggatcgc ctggtaggtg gccccgccgt tatcctcggc
      121 ctgggcggcc tggtagcgca aggcgccgct ggcgttttgc aggcggacca ggaaatcgag
      181 cgggccggcc ccgccctgtt gccacgccgc cgggtcctcg cccacggcga tcagcccaat
      241 gaccaccatg ccggtggagt tgctgtcgct ggccgcatcc ggccccttct ggtaggcgaa
      301 cccgccgtcg ctgttctgct gcgcgtgcag gtaggccagc gccttcggca ccaccgggtc
      361 gcttttggct ccggcggcgg ccagcgcctg taaggccagc ccggtggtgt tggtgtcggc
      421 gctgcccggc ccggttgagc cgtcgaacgc ccagccgccc tcgctgccct ggctttggcg
      481 cagccagtcg agcgccttcg cgcccagcgg gcggcccagg tcggtcaggg ccaggatgga
      541 cagcgcgtgc ccggtcacgt ccttgccgta ctggcccgtc gcggcatcat agccgcccgt
      601 gatcacctgc tccaggtcca cgccgcccac ctggtgcata tcggcggtgg cgccggtggc
      661 cgcgtcgaag gccaggatca gcttggccgc cgcgcccggc gtcttggcat acgtggcggc
      721 ctgcccggcc agataatcga gcgcgctctt ggtgccggcg ctgctggggt tgctgcgccc
      781 cgccgccacg agcgcgatca ccgcgtccgc cgtcgagccg acgccgaagc ccggatacgc
      841 tccggtggcc tgttgctgcc cggccaccca gcttgccgcc ttctgcacgg cgccgtcata
      901 ggcgcccgcg gcccgcgcgg cgggcgccgc cgggaccacg gcggccagca gcgcccccag
      961 tcccagcacc agggccgccc cgcgcggccc cttgtgtgtg ttgcgattca tgtctactcc
     1021 tcccatatga cctgttagaa cgctgtcact ctgagcggag cgaagagtct cggcttggaa
     1081 ccccaggaga cggagattct tcgctgcgct cagaatgaca attgctcagc gtacttgtca
     1141 ctctgagcgg agcgaagagt ctcggcgttt ggcatacaag acgagattct tcgctgcgct
     1201 cagaatgaca attgcccggc gatggttccg gggcaacaaa aaaccctctt caccaggaag
     1261 agggtccgat gtgattcgat aggatacaac cggccgtgtg gcggccaggc tcccaccccc
     1321 tttcgcgcga aggatgtgga gcaaccacag tggtgggtat cctggctcgc cgcccggcat
     1381 ggatcgggcg gcttacagtt gcggcacagc gccggtctca caccggcttc ccccaacaac
     1441 ggccccacgc atccgggcgt agggcgccac tatgttgcac gatgcaattg taaacgagcc
     1501 tcctcagtat agcaccatcc cctgctgcct gtcaacgccg acgcaccgct gcgggggatc
     1561 gccaggcccg gcaacgatcg agcttcggct acgaagcccg cccgcgcggc ctatgcgtac
     1621 gtgatggaac ggggtccgga tctctccctg gttcgtagcg gcgcgattta tcgcgcctgc
     1681 cgccccaccc ggatgccgcc cccgcgccga ttcccagccc gcgcaggcgg gcttcgtaag
     1741 ggtagcccga cccttgaggg tcggggtggc gcgcccgatg tggtatgcat attgctttta
     1801 gtatcgctga gcctggaggc atactcgacc ggaagccatc ggtcgcaatt gaactgctca
     1861 gtccgcttca cgcacgagat accagcgtgt acagggcttg tctcatcggc tcgactagct
     1921 accgcatggc ggtgggcgca ttggttcggg tagggcgcga ccttgacccg agccgtgggt
     1981 tgaagcacag tatgcagact gcgcggtatg tggcctgaag cgatggcacc ggcctgcttc
     2041 aatacccatt tatcaaggag gataccatgg ctacagtagt tggtttgttc gacaacagta
     2101 cgcaggcgca gggggctatc gagcaactgc gcgcggcggg aatcccttcg gagcatatct
     2161 cgctggcgat gcgtgaccgt agcgaagcgg ccgccgtggc cgaagaaagc gaagtcggca
     2221 ccggcgtagc gaccggcgcg gtcggcggcg gcattctggg cggtctggcc ggcctgctgg
     2281 tcggcatcgg cgccctggcg attccgggca tcggcccgct cgtggccgcc ggcccgctgg
     2341 cggcgacgct gctgggcgcc ggcatcggcg cggccaccgg cggcctgatc ggcgccctgg
     2401 tgggcgcggg cgtgcccgaa gaagaggccc agttgtatca ggcgggcgtc gagcgcggcg
     2461 gcgtgctcgt caccgtggac gccgacccgc agcaggaagc ggaggtccgc gacatcctcg
     2521 accgcagcgg tatgcgcgat gtgcgcgccg acgcggcgac ctggaacgag aatcccgagt
     2581 atcgctacgg ccggcgcacc gacgagtccg tggcaccgga agctgtcggc ggcgccggtg
     2641 gggccgtggc cggcggcgtg atcggcgcgg cggtcggcgg accggtcggc gcggcggcgg
     2701 gggccgccat tggtggggcc gcgggtgccg gcgcggggca cgaagtggct gagcccgaga
     2761 cgcatccggg cggctcggag gtcggcgggg cggcgggagc cgtcaccggc ggggtcatcg
     2821 gctcggtggc cggcccggtc ggcgcggcgg caggcgccgc catcggcggc gccgtcggcg
     2881 gcgcggcggg cgagggcgcc acggacgcgg cgtcggacac gaccacgcgc cccggctata
     2941 ccacgtcgga cgtgaccacg gacacgacgc ccggttacac gggtagcgcg cccacctata
     3001 c
//
LOCUS       DASUYC010000748         2159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_351639,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000748 DASUYC010000000
VERSION     DASUYC010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351639"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VF276_12305"
     CDS             <1..344
                     /locus_tag="VF276_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607688.1"
                     /translation="DLQQTNYLFHKSDARAIDFEDCCFHYFLFDMTVTLFELTDRPWG
                     EELGTHFLRGYRARRPLPPDHDAGIRLFTAIRLGKRINYLAHAPDPALRAQAPAWVAR
                     AVPWLEEFLAE"
     gene            799..1887
                     /locus_tag="VF276_12310"
     CDS             799..1887
                     /locus_tag="VF276_12310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6607689.1"
                     /translation="MQLDINGDSSPRASVVIPARNAARTIPATLDALRRQTYPADRLE
                     VIVVDDGSTDATGEVAAAGGARVLRQAPAGPAAARNRGAAAATGDIILFTDADCEPTP
                     TWVARMLAPFADPAVAGVKGAYRTRQRSLVARFAQVEFSDRYRRQLRGPTIDLVDSYA
                     AAFRRGVFQAAGGFDARFPAANNEDVELSYRLAESGARLVFAPGAVVYHQHPATLRRY
                     LRVKLGRGYWRVLVYRMYPGKALKDSYTPQVLKLQIALAGVLVALAPVALVWRPARRV
                     AAAVAAGLLASTAPFTAFVAREDPDVAPWTPALVVLRALALGLGTAAGIAGRGPGLTA
                     HPAPAAPGAAQPLGEDPATPAVETLAHA"
     gene            1880..>2159
                     /locus_tag="VF276_12315"
     CDS             1880..>2159
                     /locus_tag="VF276_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607690.1"
                     /translation="MRRTAEAPVTLRAVPAVPCPPASTEAPAAAPRRALPPARATARD
                     RAPRRALLLTTVPGALPLTYPAPLGAGLTAVGCCALDDPAAPVAEALAL"
BASE COUNT          282 a          837 c          731 g          309 t
ORIGIN      
        1 ccgacttgca gcagaccaac tacctcttcc acaagagcga tgcgcgcgcc atcgacttcg
       61 aggattgctg cttccactac ttcctgttcg atatgaccgt gaccctgttc gagttgaccg
      121 accggccctg gggcgaggaa ctgggcaccc acttcttgcg cggctatcgc gcccggcgcc
      181 cgctgccgcc cgaccacgac gccggcatcc gcctcttcac cgccatccgc ctgggcaaac
      241 gcatcaacta cctggcccac gcccccgacc cggccctgcg cgcccaggct ccggcctggg
      301 tcgcccgcgc cgtgccctgg ctggaggagt tcctggccga ataggcccaa tgccgggatg
      361 ccgctgtagg ggccggtttg aaaccggccc ggtggtgaga tcgccgctgt aggggccggt
      421 ttgaaaccgg cccggcgccg gggatggggg aagggggatg atacaggagg ggccggcgga
      481 gcctccgggg ggaatagcct gccgccggct gttcctgctg acctaactat aggtccgact
      541 attgctcatt gcacaggcca aaaactgctc attttcgtgc ggatagctgc ctgttctcgg
      601 tgcaccgcga agaaacgaag gacgcgaaga gcacgatgcg tataaacggt tgtcgcatcc
      661 ttcgcgcctt cgcggtgaat tcaaacccgc cggcaagttc gcgcgttgac aagcgggaaa
      721 accgaccgtt ataatcgggc gggggccggg cgcggcacgc cgccgccccg ccatgggcgg
      781 cccccggcag gagcgtggat gcagcttgac ataaacggag acagcagccc gcgggccagc
      841 gtggtgatcc cggcccgcaa cgccgcgcgg accatcccgg cgaccctcga tgcgctgcgc
      901 cggcagacct acccggcgga ccggctggag gtcatcgtgg tggacgacgg ctcgaccgac
      961 gccacagggg aggtggccgc cgcgggcggc gcccgcgtgc tgcgccaggc gcccgccggc
     1021 cccgccgctg cccgtaaccg cggcgccgct gccgccaccg gcgacatcat cctcttcacc
     1081 gacgccgact gcgaaccgac ccccacctgg gtcgcgcgga tgctggcgcc ctttgccgac
     1141 cccgccgtgg ccggagtcaa gggcgcctat cgcacccgcc agcgcagcct ggtcgcccgc
     1201 ttcgcccagg tcgagttctc cgaccgctac cgccgccagt tgcgcggccc gaccatcgac
     1261 ctggtcgact cctatgccgc cgcctttcgc cgcggcgtct tccaggcggc ggggggcttc
     1321 gacgcccgct tccccgccgc caacaacgag gacgtggaac tgtcctaccg gctggccgaa
     1381 agcggcgcgc gcctggtctt tgcgcccggc gccgtcgtct accaccagca cccggccacg
     1441 ctgcgccgct acctgcgggt gaagctgggc cgcggctact ggcgcgtgct ggtctaccgc
     1501 atgtatcccg gcaaggcgct caaggactcg tacacgccgc aggtgttgaa gctgcaaatc
     1561 gcgttggcgg gtgtgctggt ggcgctggcg cccgtggcgc tggtctggcg cccggcccgc
     1621 cgcgtggccg ccgccgtggc cgcggggctg ctggcgtcca ccgcgccgtt caccgcgttt
     1681 gtcgcccgcg aagaccccga cgtggcgccc tggaccccgg ccctggtggt gctgcgcgcg
     1741 ctggcgttgg gcctgggcac cgccgccggg atcgcgggcc gcggccccgg cctgaccgca
     1801 catcccgcgc cggctgcccc cggcgccgcg cagcccctgg gcgaggaccc ggccaccccc
     1861 gccgtggaga ctctggctca tgcgtagaac cgctgaggcg cccgtcaccc tgcgggcggt
     1921 ccccgccgtg ccgtgcccgc ccgcctcaac agaggcaccc gctgccgcac cccgccgcgc
     1981 cttgccgccc gcgcgggcca cggcgcgtga ccgggcgccg cgccgcgcct tgctgctgac
     2041 caccgtgccc ggcgcgctgc cgttgaccta cccggcaccg ctgggcgccg gcctcaccgc
     2101 cgtcggctgc tgcgctctcg acgacccggc ggcgccggtg gccgaggcgc tggccctta
//
LOCUS       DASUYC010000749         2178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_616570,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000749 DASUYC010000000
VERSION     DASUYC010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616570"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1205)
                     /locus_tag="VF276_12320"
     CDS             complement(<1..1205)
                     /locus_tag="VF276_12320"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX6607691.1"
                     /translation="MSADDLIKYLSWFVYIVIFVQTAATAIRRPLRTNIDIALFFTVS
                     TVIIASSTGVAVGLLPQNNTLINSVNGALLLSLAYMSMRLVDDFAEVAPWLMRLALGG
                     LAVSVIGTFVLAPEPPPGFALLELIYLIGFLLYGVVAFVRESRASNGVTRRRLRLAAA
                     GSIWLCVDLVFAGLRLFVPAWAGLWGALADVAGLASGVCYFLGFAPPSLLRRAWQEPE
                     LRRFLSRAAQLPRLPDTPAILREMERGAATSLGASHASIGVWDADAGVLQFTYQGRTI
                     DLAPTPETITGRAFLTQQPLFRPEVDLTNPVQAEAHQLYDVMAVLVAPITAGDTRLGA
                     LVVYSSRAPVFAEDDLALVQLLADQAAVVLESRALIDEAARVRAREEVTRLKEDFLSA
                     AAHDLKTPLT"
     gene            complement(1202..1906)
                     /locus_tag="VF276_12325"
     CDS             complement(1202..1906)
                     /locus_tag="VF276_12325"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018352723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6607692.1"
                     /translation="MDTPSWFLDELAHAGREHLDPGYVPTYDAKAGTDPAEDLALLRA
                     QGLTAGDTLVDLGAGTGTFALAAAPYCRRVVAVDVSPVMLARLREKAAACGVANIECV
                     QAGFLSYTHQGAPADFVYSRHALHHLPDFWKALALARIAAMLREGGVLVIRDLIFSCD
                     LAETPQVVGSWLAKAPADPAGGWTRAELATHLREEYSTFSWLLEPMLARAGFAIQAAQ
                     HSESRTHSAYTCVKTS"
BASE COUNT          382 a          737 c          766 g          293 t
ORIGIN      
        1 gtcagcggcg tcttcaggtc gtgggccgcc gccgagagga aatcttcctt gagccgggtc
       61 acctcctcgc gggcgcgcac gcgggccgcc tcgtcgatca gggcgcggct ttccagcacc
      121 acggcggcct ggtcggccag caactgcacc agcgccaggt cgtcctcggc gaagaccggc
      181 gcccgtgagg aatagacgac cagggcgccg aggcgtgtgt cgcccgccgt gatcggggcg
      241 accagcacgg ccattacgtc gtagagctgg tgggcctcgg cctgcaccgg gttcgtcagg
      301 tccacctcgg gccggaacag cggttgctga gtcaagaatg cccgaccggt gatcgtctcc
      361 ggcgtgggcg cgagatcgat cgtccggccc tggtaggtga attgcaagac cccggcgtct
      421 gcatcccaca cgccgatgct ggcatgggac gcgcccagcg aggtggccgc gccgcgctcc
      481 atctcgcgca ggatggccgg ggtgtcgggc agccggggca gctgggcagc ccggctgagg
      541 aaccggcgca actccggctc ctgccacgcc cggcgcagca gcgacggcgg cgcgaagccg
      601 aggaaatagc agacacccga cgccagtccc gccacatcgg ccagcgcgcc ccacagcccg
      661 gcccacgccg ggacgaacag gcgcaggccg gcgaacacca gatccacgca cagccagatc
      721 gagcccgcag ccgccagccg cagccgccgc cgcgtcaccc cgttggacgc ccgcgactcg
      781 cgcacgaacg ccaccacgcc gtacagcaaa aacccgatca ggtagatcag ttccagcagc
      841 gcaaagcccg gcggcggttc aggggccagc acaaaggtgc cgatcacgct gacggccagg
      901 ccgcccagcg ccagccgcat cagccagggc gccacctcgg caaaatcgtc gaccaaccgc
      961 atcgacatat aggccaggct caacagcagg gcgccgttca ccgagttgat cagcgtgttg
     1021 ttctgcggca agaggcccac ggcgacgccg gtgctcgacg cgataatcac ggtggacacg
     1081 gtgaaaaaca gcgcgatatc gatattcgtc cgcaacgggc gccggattgc cgtggccgcg
     1141 gtctggacga agatcacgat atagacgaac caggacaggt atttgatcag gtcgtcggcg
     1201 ctcatgatgt tttcacgcag gtgtaagccg agtgggtccg cgactcgcta tgctgcgccg
     1261 cctggatagc gaacccggcg cgggcgagca tcggttcgag cagccagcta aaggtgctgt
     1321 actcctcgcg caggtgggtc gccagttcgg cgcgcgtcca gccccccgcc ggatcggcgg
     1381 gcgccttcgc cagccaggag ccaaccacct gcggcgtctc ggcgagatcg caggagaaga
     1441 tcaggtcgcg gatgaccagg acgccgccct cccgcagcat ggcggcgatc cgggccaggg
     1501 cgagggcctt ccagaaatcg ggcaggtggt gcagggcgtg acgcgagtag acaaaatcgg
     1561 cgggggcgcc ctgatgcgtg tagctgagga agcccgcctg cacgcactcg atattcgcca
     1621 cgccacacgc ggcggccttt tcgcgcaggc gggcgagcat caccggcgac acgtccacgg
     1681 cgaccacgcg ccggcaatac ggcgcggcgg ccagggcgaa ggtgccggta cccgcgccga
     1741 gatcgaccag ggtatcgccg gcggtcaggc cctgggcgcg gagcagcgcc aggtcctccg
     1801 ccgggtcggt tcccgccttc gcgtcgtagg tgggcacata gccaggatcg agatgttcgc
     1861 ggcccgcgtg ggccagttcg tcgaggaacc aggacggcgt gtccatgggt tgtgtgcgcg
     1921 tctccttcag ggggccgggg ggctgcgtcg cctctaccca ccggctccgg ttacggcgcg
     1981 aaccacaggt ctacaatggc gcggtccacg aaggcgcctt gctgcaactt taatgccaca
     2041 gattcggaca ggcgccacga gcagggatca ggtcagggcg atccttcgcc gtcgccgtgg
     2101 gcgatcagcg gggtgagcac cagcggggcg atgttggggt agccggcgat atggggttcg
     2161 agatcggcgg cgcggctg
//
LOCUS       DASUYC010000750         2354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1681989, whole genome shotgun sequence.
ACCESSION   DASUYC010000750 DASUYC010000000
VERSION     DASUYC010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1681989"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /locus_tag="VF276_12330"
     CDS             <1..1046
                     /locus_tag="VF276_12330"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX6607693.1"
                     /translation="PHSYNPPSGYIATANNRITGADYPYFLGREWSTGYRARRIADLL
                     EARPKLTLDDFAAIQNDLYTIPGARLSRVLVDRLSQRVPPPGRRGAMRAQALAYLDAW
                     DGRMTVNSVAAPIYEVTLHFLTRYVYGRAFQDEMLLDQYLGSATSPATTSTHYVARAV
                     PVLLRALEDNDLDWLRAIGRDPVADADLTWEELLEKALDDGIRLLRRRMGHDMKRWRW
                     GRLHRISLAHPMGLGPFARVFNPPLIPMPGDRDTIFMSSELPNSPLSAAGNSVSYRQL
                     FDLGSWDTGRVVLPGGEAGQPASPHYSDMVRLWQQGRYAPLPFSPAAVAAATAAELVL
                     TPDQETGAPDESA"
     gene            complement(1203..>2354)
                     /locus_tag="VF276_12335"
     CDS             complement(1203..>2354)
                     /locus_tag="VF276_12335"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEX6607694.1"
                     /translation="RSAVRPLTRFDATPFKSRVAAQIDDFDPLALLDARTARRMDRYS
                     QFAVVTALQAVADAGLEIADSCRARGGVYLGSALGGLAFAEGEHVQFQAGGIRAVNPI
                     LALSVFGAAASANAAMALGLRGPNMTNANSCASGALAIGQAFDLIRRGEADFMLAGGV
                     EVPLAPLIFGAFALIGALSGHNATPETASRPFDRTRDGFVMAEGAATLLLEDRAHALA
                     RGAHIYGEITGFAMTTDAYHMTAPLPSGAEAARVMRGALADAGIAPAQVGYINAHGSG
                     TPLGDRVEALALRQVFGDDLGGIPVSGTKGMHGHALGASGAMEAAICALCLEHGWLPP
                     SINIATPDPECALPFIGGDGLERRVDYILSNSFGFGGLNVGLVFARYRK"
BASE COUNT          354 a          875 c          791 g          334 t
ORIGIN      
        1 tgccgcacag ctacaacccg ccatcaggct atatcgccac cgccaacaac cgcatcaccg
       61 gggcggacta cccctacttc ctgggccgcg agtggtccac gggctaccgc gcccggcgca
      121 tcgccgacct gctcgaagcc cggcccaagc tcacgctgga cgatttcgcc gccatccaga
      181 atgacctcta caccattccc ggcgcccggt tgagccgggt gctggtggac cggctgagcc
      241 aacgggtgcc gccgccgggc cggcggggtg ccatgcgcgc gcaggcgctg gcctacctgg
      301 acgcctggga cgggcggatg accgtcaaca gcgtggccgc gccgatctac gaagtgacgc
      361 tgcacttcct cacgcgctat gtctatggcc gggcgttcca ggacgagatg ctgctggacc
      421 agtacctggg cagcgcgacc tcgcccgcca ccaccagcac ccactatgtc gcccgcgccg
      481 tgcccgtgct gctgcgcgcg ctcgaagaca acgacctcga ttggctgcgc gccatcgggc
      541 gcgatcccgt ggccgacgcg gacctgacct gggaggaact gctcgaaaag gcgctcgatg
      601 acggcatccg gttgctgcgc cgccgcatgg gccacgacat gaagcgctgg cgctggggcc
      661 gtctgcaccg catctctctg gcccatccca tgggcctcgg cccgttcgcc cgcgtgttca
      721 acccgccgct gatccccatg cccggcgacc gcgacaccat cttcatgagc tcggagttgc
      781 cgaactcgcc gctgtcggcg gcgggcaaca gcgtctccta tcgccagttg ttcgacctcg
      841 gctcgtggga cacagggcgg gtggtcttgc ccggcgggga ggccggccaa cccgcctcgc
      901 cgcactacag cgacatggtg cgcctgtggc agcaaggccg ctatgcgccc ctgcccttct
      961 cgcccgccgc cgtggccgcc gccaccgccg ccgaattggt cctcaccccg gatcaggaga
     1021 ccggggcgcc ggacgagtcc gcctgatagc cggccaccgg aatttggcga tgtaggggcc
     1081 ggtttcaaac cggccccgcc tctgccggca tcgcgggata tccttaatag cccgccacgc
     1141 cgattttgcg atgtaggggc cggtttgaaa ccggcccgtg aaggtatcac ggcggtgggc
     1201 tactacttcc ggtagcgggc gaagacgagg cccacgttga ggccgccgaa gccgaacgaa
     1261 ttactcagga tatagtccac ccgccgctcc aggccgtcgc cgccgatgaa cggcagggcg
     1321 cactccgggt ccggcgttgc gatattgatc gagggcggca accagccatg ctccaggcag
     1381 agcgcgcaga tggctgcctc catcgccccg ctggccccca gcgcgtggcc gtgcatgccc
     1441 ttggtgccgc tgaccgggat gccgcccagg tcgtcgccga acacctggcg gagcgccagc
     1501 gcctccaccc ggtcgcccag cggcgtgccc gagccgtggg cgttgatata gccgacctgg
     1561 gcgggcgcga taccggcatc ggccagcgcc ccgcgcatca cccgcgccgc ctcggccccg
     1621 ctgggcagcg gcgccgtcat gtggtaggcg tcggtggtca tggcgaagcc ggtgatctcg
     1681 ccgtagatat gggcgccgcg agccagggcg tgcgcgcgat cctccagcag cagcgttgcg
     1741 gccccctcgg ccatcacgaa gccgtcgcgc gtgcggtcga acggacggct ggcggtttcc
     1801 ggcgtggcgt tgtgcccgga cagagcgccg atcagcgcga aagcgccgaa gataagcggc
     1861 gccagcggca cctccacgcc cccggccagc atgaagtccg cctccccgcg ccggatcagg
     1921 tcgaacgcct ggccgattgc cagcgcgccc gacgcacagg agttggcgtt ggtcatgttc
     1981 ggcccgcgca gccccagcgc catcgccgca ttggccgagg ccgccgcgcc gaacacgctc
     2041 agcgccagga tggggttgac ggcgcggatg ccgcccgcct ggaactgcac atgctcgcct
     2101 tcggcgaagg ccagcccgcc cagcgcgctg cccaggtaga ccccgccccg tgcccggcag
     2161 gaatcggcga tctccaaccc ggcgtcggcc accgcctgca gcgccgtcac cacggcgaac
     2221 tgcgagtagc ggtccatgcg ccgcgcggtg cgggcatcga gcagcgccag cgggtcgaag
     2281 tcgtcgatct gcgcggccac tcgcgatttg aacggcgtgg cgtcgaagcg ggtcagcggg
     2341 cgcacggcgg agcg
//
LOCUS       DASUYC010000751         3724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_114050,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000751 DASUYC010000000
VERSION     DASUYC010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114050"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VF276_12340"
     CDS             <1..224
                     /locus_tag="VF276_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6607695.1"
                     /translation="SEGLAPIIIEHIVAALRRLSEYITVLLVEQNFRMAAQLADQYYI
                     LDDGRSVQAGRMADLVQDQALITRYLGAA"
     gene            317..559
                     /locus_tag="VF276_12345"
                     /pseudo
     CDS             317..559
                     /locus_tag="VF276_12345"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
     gene            914..1756
                     /locus_tag="VF276_12350"
     CDS             914..1756
                     /locus_tag="VF276_12350"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX6607696.1"
                     /translation="MPLRTPGETWILGLSSNVRFLLALVIGAGTGAALGAGIEWSMIR
                     PLYTRPIYQVLLTLGLVFVGTEAVKGIWGPAGFYMDIPAFFNSNSKNCPSPNLLAWFS
                     DHCASIDVLGRPFPSYRIFIIVLGLVIFVAIALLLKYTRMGMIIRAGVQDSEMVQALG
                     INVRRVFTLVFALGAGLAALGGVAAAPFIGVSPGLGQDFLLLAFIAVVIGGMGSYPGA
                     AIGALLVGLARAFGDQLVLSGVHLPGMEDAIKFSPSIARASTVLLMAVVLLVRPAGLF
                     GRKE"
     gene            1753..2790
                     /locus_tag="VF276_12355"
     CDS             1753..2790
                     /locus_tag="VF276_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX6607697.1"
                     /translation="MIALRERLPRAWLGALALLVLVLFPFLLAALTGEPVDSGAPKFW
                     QGLLIQVFILAVYAMSYDLLLGYTGILSFGHAMFFGTGAYATGILLTHAGWALWQVAL
                     AVLGVAIIQGLVIGVLSLRVRGVYLAMVTLAFAQMFFILAEATDFRDLTGAEDGLHGI
                     PIPAWMSPTDERLRFYFIALAFCVVMYLIARRVVESPTGRVMVAIRENEARAQMIGYN
                     TFVYKLIAVTLSGTLAALAGLMNALWNVNANPAMLSVTTTINALLMVIIGGVGTLVGP
                     LLGAGVLQLLGYWLNSTFGPRWPLIFGIVYILIVLFFPYGLVGTWQLRGGAWWRTWRD
                     RLRGVPARRVL"
     gene            3360..>3724
                     /locus_tag="VF276_12360"
     CDS             3360..>3724
                     /locus_tag="VF276_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX6607698.1"
                     /translation="MKARPVLRILALGAFLFALLPLAGGPAPAAVAAGNKNSLSKHDR
                     ELLADAQARGDSTVTLLIAAVPGGNNTVVTGLTRLGATVRYQDGDVSYIRAVVPIGKA
                     DAAAQLAGVQAANLDEVIPL"
BASE COUNT          497 a         1265 c         1255 g          707 t
ORIGIN      
        1 cgagcgaggg cctggccccg atcatcatcg agcatatcgt ggccgcgctg cgccgcctgt
       61 ccgagtacat caccgtgctg ctggtcgagc agaacttccg catggccgcg caactggccg
      121 accagtacta catcctggac gacgggcgca gcgtgcaggc gggccgcatg gccgacctgg
      181 tgcaggatca ggcgctgatc acgcgctacc tgggcgccgc gtaggccccg gcgaggaaat
      241 cgttatggtt gatgtgttgc gccgcaatcg cgccgccgcc gtgaccgccg tcgtggtggt
      301 gctgctgctg gtgctgttga gccggcagta cgagccgcgg gtcacggcca gcatcgtgct
      361 gtcgggcctg accctcggcg cgctctattt cctggtgacg gcgggcctct cgctcatctt
      421 cggcctgatg gacgtgctca acttcgcgca cggcacgctg ttcatggtcg gcgcctatgc
      481 cgggtacacg ctctacgcca acccgcgcat gatcctcaac accgcgccgt tcgcgctggt
      541 gctggcgggc ggcgcgctgc tgggcgcgtg ggccgcgcgg cgctggggtg gacggctgcc
      601 ggccaatctg ccgcgctggg tcgcctgggc gccggcgctg ctcggcgcgc tgctgctgct
      661 gccggggctg ggcggcttcg acctgctcaa gctggctgcc agcagtgcca tcgccagcgg
      721 cggcgccgtg cccaccgcgg tggcccagga gccggacggc gcgttcgccc tgcggctgat
      781 ctggctggtc ggggccgggg tgctgttcgg cgtggcgccg ggcctgcaca ccgcgcggcg
      841 acccggtacc gtggcgcccg gcgggcggcg cctgctggcc ggcgtggcgc ttatcttgct
      901 ggcctgcgtc ctggtgccgc tgcgcacgcc tggcgagacg tggatcctcg gcctgtcgtc
      961 gaacgtgcgc ttcctgctgg ccctggtcat cggcgcaggc accggggcgg cgctgggcgc
     1021 gggtatcgag tggagcatga tccgcccgct ctacacacgg cctatctacc aggtgctgct
     1081 cacgctgggc ctcgtcttcg tgggcaccga agccgtcaag ggcatctggg ggccggcggg
     1141 cttctacatg gacatccccg cgttcttcaa cagcaacagc aagaattgcc cctcgcccaa
     1201 cctgctggcc tggttcagcg accactgcgc gtccatcgac gtgctggggc ggcccttccc
     1261 cagctaccgc atcttcatca tcgtgctcgg cctggtgatc ttcgtcgcca tcgccctgct
     1321 gctgaagtac acgcgcatgg gcatgatcat ccgggcgggc gtgcaagaca gcgagatggt
     1381 gcaggcgctg ggcatcaacg tgcgccgggt cttcactctg gtcttcgcgc tgggagcggg
     1441 cctggccgcg ctgggcgggg tggcggccgc gccgttcatc ggcgtcagcc ccggcctggg
     1501 ccaggatttc ctgctgctgg ccttcatcgc cgtggtcatc ggcggcatgg gcagctatcc
     1561 cggcgcggcc atcggcgcgc tgctggtcgg cctggcccgc gccttcggcg accagctcgt
     1621 gctgtccggc gtccacctgc cgggcatgga ggacgcgatc aagttctcgc cgtccatcgc
     1681 ccgcgcctcg accgtgctgc tgatggccgt cgtgttattg gtccgccccg ccgggttgtt
     1741 tgggaggaaa gaatgatcgc cctgcgtgag cggttgccgc gcgcctggct gggcgcgctg
     1801 gcgctgctgg tcctggtgct gttcccgttc ctgctggccg cgctgaccgg ggaaccggtg
     1861 gatagcggcg cgcccaagtt ctggcaaggg ctgctgatcc aggtctttat cctggccgtc
     1921 tatgccatgt cctacgacct gctgctgggc tacacgggca tcctctcctt cggccacgcc
     1981 atgttcttcg gcaccggcgc ctatgccacc ggcatcctgc tgacccacgc cgggtgggcg
     2041 ctctggcagg tggcgctggc cgtgctgggg gtggcgatca tccagggcct ggtcatcggc
     2101 gtgctctcgc tgcgcgtgcg cggcgtctac ctggcgatgg tcacgctggc cttcgcccag
     2161 atgttcttca tcctggccga ggccaccgac ttccgcgacc tgaccggcgc cgaggatggg
     2221 ctgcacggca tccccatccc ggcctggatg agccccaccg acgagcggct gcgcttctac
     2281 ttcatcgcgc tggccttctg cgtggtgatg tatctgattg cccggcgcgt ggtggagtcg
     2341 ccgacgggcc gggtcatggt cgccatccgc gagaacgagg cgcgcgccca gatgattggc
     2401 tacaacacct tcgtctacaa gctgatcgcc gtcacgctct cgggcacgct ggccgcgctg
     2461 gccgggctga tgaacgcgct ctggaacgtg aacgccaacc cggccatgct cagcgtgacc
     2521 acgacgatca acgccctgct gatggtcatc atcggcgggg tgggcacgct ggtcgggccg
     2581 ctgctgggcg cgggcgtgct gcaactgctg ggctactggc tcaacagcac cttcgggccg
     2641 cgctggccgc tcatcttcgg catcgtctac atcctgatcg tgctcttctt cccctacggg
     2701 ctggtcggca cctggcagtt gcgcggcggg gcctggtggc gcacctggcg cgaccggctg
     2761 cgcggggtcc cggcccggcg cgtgctttag cggggttcct gtggctaaac cggctcaggg
     2821 taacagccgc aacgcaaatc gggttctcgg actagaggca cgcagcggtt gccatccgcc
     2881 acgagcatcc cgcttcgcat tttgacctat ttccagcagc ctgttcgatc gagccggata
     2941 agcctgttcg ggtctaccgg gctattggtc aaatttgtgt caatgattca agcacaatga
     3001 tgcttggctt gacactgcaa cggttatacg gtaacattct cctcgtgatc gactaatcat
     3061 gaccgggcga accggcatcc gtggtatccg gctgcgggtg ccggcgtgga tcaggttagt
     3121 caacatcact gtagaccgcc catcttggga ggaaggcggt cttcattccc tcactgctgt
     3181 atagcgacat gcttcaaata aagaccgata gaacgccgga aacgcgggcg cggttcctgg
     3241 ttagtctaac ctagaaggtc aacccccgat tatcgaatga ggttcgcctg gcttgccggt
     3301 gcccggatca ccgcgggcta gcgaccccat gcctcacttc cacgaggagg agctcatcgt
     3361 tgaaagctag accagtcctt cgcatcctcg cgctcggcgc atttctgttc gccctgctcc
     3421 cgctggccgg ggggccggca ccggcagcag tggccgccgg gaacaagaac tcgctctcca
     3481 agcacgatcg cgaactgttg gcggatgccc aggcgcgcgg cgacagcacc gtcaccctgt
     3541 tgatcgcggc tgtccccggc gggaacaaca ccgtcgtcac cggcctcacc cggctcggcg
     3601 ccaccgtgcg ttaccaggac ggcgatgtca gctatatccg cgccgtggtg ccgatcggca
     3661 aggccgacgc ggccgcccaa ctcgccggcg tccaggcggc gaacctggac gaggtgatcc
     3721 cgct
//
LOCUS       DASUYC010000752         3238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1268853, whole genome shotgun sequence.
ACCESSION   DASUYC010000752 DASUYC010000000
VERSION     DASUYC010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268853"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(220..1158)
                     /locus_tag="VF276_12365"
     CDS             complement(220..1158)
                     /locus_tag="VF276_12365"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6607699.1"
                     /translation="MPRGAGPPRRMTGDEFEIRPARPADREAVLAFCAQTWEWGDYIG
                     EVWDQWLADREGVLVVGEVGGQVMGVDKLTFLAPGEAFFEGLRIAPAYRGRGYAGRFQ
                     RFMLAEAARRGGQVLRFLTAADNTPIHKNAARDAFLRGPALLPWQATPAAEARAPAAM
                     PLEPGPAAEAVWAALPQSPMWAATGGLVSHGWHVQAWTRTCWDALLAAGHLFTLPTRD
                     ASRVPGLIALIPEQHYPDAVWLAYLDAGALDPADLAALAGAARQVAAARGWAELHAML
                     PRIPAIEAGLQAAGWTPRSDEGTMLVFEKTLSPPAP"
     gene            complement(1125..1742)
                     /locus_tag="VF276_12370"
     CDS             complement(1125..1742)
                     /locus_tag="VF276_12370"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006320979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEX6607700.1"
                     /translation="MPDTIPPQPGPHGPGPAGSIEVICGSMFSGKTEELIRRIKRAQI
                     ARQRVQVFKPRLDNRYAAEQVASHNGLLYQAISAGDTAEIRAALDTGATVVAIDEVQF
                     FDWDIVDLCRELADRGVRVLVAGLDLDFRGEPFGPMPMLMAEAEHISKLQAICVVCAN
                     AASRTQRLIDGQPASYHDPVIMVGASEVYEARCRHCHEVPGRPGA"
     gene            complement(1824..2171)
                     /gene="rpmE"
                     /locus_tag="VF276_12375"
     CDS             complement(1824..2171)
                     /gene="rpmE"
                     /locus_tag="VF276_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEX6607701.1"
                     /translation="MKPNIHPKYQTATVTCACGNSWTTRSTKAQLRTDVCSACHPFFT
                     GEQRIVDTAGQVERFMRRMQRSQERSSAPAPQPAQAAAKPAKPAKAPKPGREAARPAT
                     LEALLETPAGSES"
     gene            complement(2251..2565)
                     /gene="rpmA"
                     /locus_tag="VF276_12380"
     CDS             complement(2251..2565)
                     /gene="rpmA"
                     /locus_tag="VF276_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX6607702.1"
                     /translation="MAHKKGVGSSRNGRDSKPKMLGVKRYDGEAVLSGTIIVRQRGTK
                     IDPGTNVGVGRDHTLFATADGYVKFEHAAKENNKKRVSVYPQRAGDTAEAEAVEPTPT
                     VA"
     gene            complement(2623..2934)
                     /gene="rplU"
                     /locus_tag="VF276_12385"
     CDS             complement(2623..2934)
                     /gene="rplU"
                     /locus_tag="VF276_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004834209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX6607703.1"
                     /translation="MYAIVRTGGHQYKVAVGDQLDVEKLPVEAGEQVELNEVLMVSSD
                     DQVTVGDPLVAGARVTATVVDQHRGNKIIVFKYKPKKRYRRKMGHRQDLTRLSINAIT
                     L"
BASE COUNT          486 a         1066 c         1150 g          536 t
ORIGIN      
        1 agaattgggc gaagtggccg gcttcggtca gggcggccca atcgatgcgc ggcaggtgca
       61 tcgcgccggg ggtgaggtca gatagccccg gcacggatgc ggcagggatg ttaacttggg
      121 ccacggaagc ctccaacgaa cggcgtgcgg ttgatcaatc tgccccatca cggcagcaaa
      181 ttggagtcat accagcaaga ttcacgccag cattggggat catggcgcag gtgggctcag
      241 cgtcttctcg aacacaagca tggtgccttc gtcggatcgt ggggtccagc ccgcagcctg
      301 caagcccgct tcgatggccg ggatgcgcgg caacatggcg tgtaactcgg cccagcctcg
      361 tgcggcggcc acctgccggg ccgcgccggc cagcgccgcc agatccgcag ggtccagggc
      421 gccggcgtcg aggtacgcca gccagacggc atccgggtaa tgctgctcgg gaatcagggc
      481 gataagacca gggacccggc tggcatcccg tgtgggcaag gtaaacaggt ggcccgccgc
      541 cagcagggcg tcccagcagg tgcgagtcca ggcctgcaca tgccagccat gcgacaccag
      601 cccgccggtc gctgcccaca tcgggctttg cggcagtgcg gcccacacgg cttccgctgc
      661 gggaccgggt tcgagcggca tggcggcggg ggcgcgggcc tcagcggcgg gcgttgcttg
      721 ccagggcagc agcgccgggc cgcgcagaaa tgcgtcgcga gcggcgttct tgtggatggg
      781 cgtgttgtcc gcggcggtga ggaagcgcaa cacctgtccg ccgcggcggg cggcttcggc
      841 cagcatgaag cgctggaagc gccccgcgta gccgcggccc cggtaggccg gggcgatgcg
      901 caggccctcg aagaaggctt cgccgggcgc caggaaggtc agcttatcga cgcccatcac
      961 ctgcccgccg acttcgccga ccaccagcac accctcgcga tcggccagcc actggtccca
     1021 gacctcgccg atatagtcgc cccactccca ggtctgggcg cagaaggcca gcacggcttc
     1081 gcggtcggcg gggcgcgccg ggcggatctc gaactcgtcg ccggtcatgc gccggggcgg
     1141 cccggcacct cgtggcaatg gcggcagcgc gcctcgtaga cctcgctggc gccgaccatg
     1201 atcaccgggt cgtgatagga cgcgggctgg ccgtcgatca accgctgggt gcggctggcc
     1261 gcgttggcgc agaccacgca gatcgcctgg agcttgctga tgtgctcggc ctcagccata
     1321 agcatgggca tggggccgaa cggctcgcca cggaagtcga ggtcgagccc ggccaccagc
     1381 acgcgcacgc cgcggtcggc cagttcccgg cacagatcga caatatccca gtcgaagaac
     1441 tgcacctcgt cgatggcgac gaccgtggcg ccggtatcga gggcggcgcg gatctcggcg
     1501 gtgtcgccgg cggagatggc ctggtagagc aggccattat gcgaggcgac ctgctcggcg
     1561 gcgtaacgat tgtcgaggcg cggcttgaac acctgcacgc gctggcgcgc gatctgggcg
     1621 cgcttaatcc gccggatcag ctcttcggtc ttgccgctga acatgctgcc gcagatgacc
     1681 tcgatgctgc cggcgggtcc gggaccgtgg ggccccggtt gggggggaat ggtatcgggc
     1741 ataagacccc gttacaatgg ggaaagcgcg gagcaaccgg cctgtgccgg gctgctccgc
     1801 gccccgtggg tggatacgat gtgttagctt tcgctgccgg cgggcgtttc cagcaacgct
     1861 tcgagtgtgg ccgggcgggc cgcctcgcgg cccggcttcg gcgccttggc cggcttggcg
     1921 ggcttggccg ccgcctgggc gggctgcggc gcgggggcgg acgagcgctc ctgcgaccgc
     1981 tgcatgcggc gcatgaaccg ctccacctgg cccgcggtgt ccacgatgcg ctgctccccg
     2041 gtgaagaacg ggtggcacgc cgagcacacg tcggtgcgca gttgcgcctt ggtcgagcgg
     2101 gtggtccagc tattgccgca cgcgcaagtg acggtagcgg tctggtactt cggatgaata
     2161 ttcggcttca agtttagctc cttccggcgt ttattcgccg cctgccggcg acggtccttg
     2221 ccgggcaatg gacatatgcg aacggtgtag ctaggcgacg gtcggggtgg gctccaccgc
     2281 ctcggcttcc gccgtgtcgc ccgcgcgctg cggatagacg ctgacgcgct tcttgttgtt
     2341 ttccttggcc gcgtgttcga acttcacata gccgtccgcc gtggcgaaca gcgtgtggtc
     2401 gcggccaacc ccgacgttgg tgccgggatc gattttggtg ccgcgctggc ggacgatgat
     2461 cgtccccgag aggaccgcct cgccgtcgta gcgcttcacg ccgagcatct tcggcttgga
     2521 gtcgcgcccg ttgcgcgagc taccaacccc ttttttatgg gccatgtctc tactctcctg
     2581 tgatctgccg gcgccggcaa tggtctgcgc cgcccggccg ggttacagcg taatcgcgtt
     2641 aatcgaaagc cgggtcagat cctggcggtg acccatcttg cgccggtagc gcttctttgg
     2701 cttgtacttg aagacgatga ttttgttgcc ccgatgctga tcgaccacgg tcgccgtcac
     2761 gcgggccccg gcgaccaggg gatcgcccac cgtcacctga tcatccgagc tgaccatgag
     2821 cacttcgttc agctcgacct gctcaccggc ctcgaccggc agcttctcca catctaactg
     2881 gtccccgaca gccactttat actgatggcc gcccgttctc acgattgcgt acaccttatc
     2941 ctcccaaata gaaacctaga ggtcacagcc tttaagtata cccgcggcgg gcgggattgt
     3001 caaggcgatt cgcagacgca gacagaggcg cggagcagga tgtggcctgc tccgcgcgcg
     3061 ttgcggcggg atgaacgagc ggttacgcct cttgggcggg cggacgcggg gccgggggcg
     3121 cggcggagcc ggcagccacc ggggcagccg gcgcaggcgc ggcagccgcg gtggcgggag
     3181 cgctcggcgc aggggccggg gccgggccgg tgcccgccgg tgccccatca gcatccgg
//
LOCUS       DASUYC010000753         4528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_411901,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000753 DASUYC010000000
VERSION     DASUYC010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4528
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411901"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VF276_12390"
     CDS             complement(<1..255)
                     /locus_tag="VF276_12390"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HEX6607704.1"
                     /translation="MSGRQRVPQDAWKRIIAASPEEGVQPLRNAKEIDINLIHTDPRQ
                     PRKHFDEEAMNELIASVKQRGILQPITVQRRSEGGYFVVTG"
     gene            complement(252..1133)
                     /locus_tag="VF276_12395"
     CDS             complement(252..1133)
                     /locus_tag="VF276_12395"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX6607705.1"
                     /translation="MTNTTDGRPHQTRIIAIANQKGGVGKTTTTVNLGAELAALGRRV
                     LLIDLDPQAAATYSLGIDPEELDLTLYNVMVRREGSITEIIYKTPVEGMDLVPSNLDL
                     AAAELDLFRNYTRERVLRDALAPVVGEYDYILIDCPPSLTLLTVNAFVAATEVLIPLQ
                     THLLSLRGVHRLLSTVNEVAQLNPDIKILGILPTMYDSRSNLNKEILANIDQVYSGRV
                     FKSVIRYGVVAAEAPGQGVPIRLYARNSPVARCYQELAAEIVADEPQPAPVPAPVAPS
                     ARGRKSGAGPAKGEAQA"
     gene            complement(1145..1501)
                     /locus_tag="VF276_12400"
     CDS             complement(1145..1501)
                     /locus_tag="VF276_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607706.1"
                     /translation="MTNRVFTTQEALALLADRGHDTTARVLRNIEDKLDLHVQRVGGT
                     GADDDEQGPRLYTVADLDLLERVLVLQKAGLHLIEIRAILIEHDHYVLEFHANSLRRA
                     LDLLNTIQNEWRITAA"
     gene            1838..2710
                     /locus_tag="VF276_12405"
     CDS             1838..2710
                     /locus_tag="VF276_12405"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6607707.1"
                     /translation="MASQYQKLAMAFFHPSLREDPFNPEDSARYFYLADQYREALDLL
                     LRHVLRRGGLSIIYGDVGLGKSSLLAVLRQRARTSDNFKQYVLGEILDPSQVTDAEFT
                     RQLLLAFDQKPYGSTRSDLSDQFTIFLQRQAREGKVPLLVIDEGQCLSYDGLERLRIF
                     LNYSLDDHRILQIIIFGQLELQEKVAKKRNLASRVRFTMTLEPLRSVGQVGDLIEYRL
                     RTAGLAPGVQLFAPGAIEAVYKYSGGVPRHIVNICSLCFDQAPLLNKRQIDAEIVEHV
                     ATRLMQKGTLTDGR"
     gene            2700..3092
                     /locus_tag="VF276_12410"
     CDS             2700..3092
                     /locus_tag="VF276_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607708.1"
                     /translation="MDDEANGRPSRYWIEDQMANDRAREGEGRLKQVAVYYYPKQLIP
                     LDEHLSRLNSRTRAYDGLKINRSTYLRLCGAVLMDLLNDPAFAGNMFDRLYEEAELEQ
                     RLRDKLRDLMELQRAYQDGTLRPVLPEI"
     gene            complement(3424..4230)
                     /locus_tag="VF276_12415"
     CDS             complement(3424..4230)
                     /locus_tag="VF276_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010493965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HEX6607709.1"
                     /translation="MMEASRDHNVIPRVTTPAGLPGAWVMTQTWNDLLFAHWPLPPDA
                     LTPCVPRGLTLDTFDGQAWLGITPFWISHLRARGMPSIPGTARFHEINVRTYVRGPGG
                     SGVLFLSLDAANVLAVLGARIVYHLPYFNTQATHTRAGRRIRYVSRRRHPGAPGAVFV
                     AGYEPVSGVFNARPGSLEHWLSARFRLFAQDSGGRLYKAEIRHKAWPLQEAQARIVRN
                     TMAEAHGLHLPDVPPLLHYTRRLKTFFWLPERVLPEMDTIPPDIDVKLVK"
BASE COUNT          790 a         1418 c         1404 g          916 t
ORIGIN      
        1 gccggttacg acgaaatagc cgccctcgga ccgccgctgc accgtgatcg gttgtaaaat
       61 gccccgctgc ttgacggagg cgatcaactc gttcatcgcc tcttcgtcaa agtgcttgcg
      121 cggctgccgg ggatcggtat ggatcaggtt gatgtcgatc tctttggcgt tgcgcaacgg
      181 ctgcacgcct tcttcgggac tcgccgcgat gattcgcttc caggcatcct ggggcacgcg
      241 ctggcgtccg ctcatgcttg cgcctcgcct ttcgccgggc cggcgccgct cttgcggccc
      301 cgcgcgctgg gtgcgaccgg cgcggggacc ggggcgggct gcggttcgtc ggcgacgatc
      361 tcggcagcga gttcctggta gcagcgggcc accggcgaat tgcgcgcgta gagccggatc
      421 ggcaccccct gccctggtgc ttccgccgcc accacgccat agcggatcac cgacttgaac
      481 acgcggcccg aatagacctg gtcgatattc gccaggatct ccttgttcag gttgctgcgc
      541 gagtcgtaca tggtcggcaa gatgcccagg atcttgatgt cggggttcaa ctgcgccact
      601 tcgttgacgg tggacaggag gcgatggacg ccgcgcagcg agagcaagtg cgtttgcagc
      661 gggatcagca cttcagttgc cgcgacgaag gcgttgacgg tcagcaaggt caggctgggc
      721 gggcaatcga tcagaatata atcgtactcc cccaccaccg gggccagcgc gtcgcgcagc
      781 acccgctcac gggtgtagtt gcggaacagg tcaagttcgg cggcggcgag gtccaggttc
      841 gacggcacca ggtccatgcc ttcgacgggc gtcttataga taatctcggt gatactgccc
      901 tcgcggcgca ccatgacatt atataaggta aggtcgagtt cttcggggtc gatgcccagc
      961 gaataggtcg cggcggcctg cgggtcgaga tcgatcagca gcacccggcg gcccagggcg
     1021 gcgagttcgg cgcccaggtt cacggtggtg gtcgtcttgc cgacgccgcc tttttgattg
     1081 gcgatggcga tgatccgcgt ctggtgcggg cgcccgtccg tggtgtttgt caccggcatc
     1141 tcccctaggc cgccgtgatc cgccactcgt tctgaatggt gttcaacaga tcgagcgcgc
     1201 gccgcaagct gttggcatgg aactccagca cgtaatggtc atgctcgatc aggatcgcgc
     1261 ggatctcgat caggtgcagg ccggcctttt gcagcaccaa gacccgctcc agcagatcaa
     1321 gatcggccac ggtatagagg cgcggtcctt gctcgtcatc gtcggcgccg gtgccaccca
     1381 cgcgctggac gtgcagatcg agcttgtcct cgatgttgcg cagcacccgc gcggtggtat
     1441 cgtggccccg gtcggccagg agggccagcg cttcctgggt agtaaacaca cgattggtca
     1501 tagctcgcct ttggttacgt atcccctggt ttccgcggcc aagcctttgg gatcgtgaaa
     1561 atagtcgctt agctcgcgtg agtcatatta taaccggttc tcaggcgctg tcaaattcct
     1621 gctactgtga tgctatgcca acgtactaac acgttgataa gctgttagcc ggaacatcgg
     1681 tgtcaagcct attgacttca ccaatgtttt gcagtagtat tgacatcaat gtcaaccctg
     1741 ctgttgcagc ctggttgcgg gcgttcttcc gtgcggtctg tgtccggcag cgtggcgaga
     1801 ggccgtaagc agcgagcggt gataagagag gtcgtgcatg gcgagtcaat accagaaact
     1861 ggcgatggcc ttcttccatc cctcgctgcg cgaggaccct tttaacccgg aagacagcgc
     1921 gcgctacttc tatctggccg accaataccg tgaagccctg gacctcctgt tgcgccacgt
     1981 gttgcgccgg ggtggtctgt ccattatcta cggcgatgtc ggtctgggca agagcagctt
     2041 gctcgccgtg ctgcgccagc gggcccgcac cagcgacaat ttcaagcagt atgtgcttgg
     2101 cgagatcctc gatccctcgc aggtgacgga cgcggagttc acccgccagt tgctgctggc
     2161 cttcgaccag aagccctacg gcagcacgcg ctcggacctc agcgaccagt tcaccatctt
     2221 tctccagcgc caggcgcgcg agggcaaggt gccgctgctc gtcatcgacg agggccagtg
     2281 cctgtcctat gacggcctgg aacgcctgcg gatcttcctg aactattcgc tcgacgacca
     2341 tcgcatcctg caaatcatca tcttcgggca gctcgaattg caggagaagg tcgccaagaa
     2401 gcgcaacctg gcctctcgcg tgcgcttcac catgaccctc gaaccgttgc gctcggttgg
     2461 gcaggtgggc gacctgatcg agtaccggct gcgcaccgcc ggcttggcgc ccggcgtgca
     2521 gttatttgcg cccggcgcca tcgaagcggt atataagtac tcagggggcg tgccccggca
     2581 tatcgtcaat atctgctcgc tctgcttcga ccaggcgccc ctgctgaaca agcgccagat
     2641 cgacgccgaa atcgtcgagc atgtggctac ccgcctgatg cagaaaggaa ccctgaccga
     2701 tggacgatga ggcgaacggc cgtccctcgc gctactggat cgaggatcag atggcgaacg
     2761 accgcgcgcg cgaaggcgaa ggccggctca agcaggtcgc ggtctactac tatcccaagc
     2821 agttgatccc cctggacgag cacctgtcgc gcctgaacag ccgcacgcgc gcctatgacg
     2881 ggctgaaaat taaccgcagc acgtatttac ggctctgcgg cgccgtgctg atggacctgc
     2941 tcaacgaccc cgccttcgcc ggcaatatgt tcgatcggct ctacgaagag gcggaactcg
     3001 aacagcgcct gcgcgacaag ctgcgcgacc tgatggagct gcagcgcgct taccaagacg
     3061 gtaccctccg cccggtcctg ccggagatct aaccctccgg ccagggccag ccattttccc
     3121 ctcaccgcaa tttcctcatt agtatgttgg tataataata agcatgttta ctaaggaatt
     3181 agtttgcctg cactcttgct ttgccctgcg ccggcactat tttatgctgg gcgcttttcc
     3241 atagaccggg tttggcactc tccgctccgc tcagaatctc gtcatttcct caccaggcca
     3301 gagattcttc gctgcgctca gaatgatgat gcttgtgtga cccgccgatc cgcttatcgt
     3361 tctaccgtct cgtggccgga gcgcacgatc cggcgactag gtcgtcgcat ccggttctca
     3421 agcttatttt accagcttga catcaatgtc agggggtatg gtgtccatct ctggcagtac
     3481 ccgctctggg agccagaaga acgtcttgag gcgccgggtg taatgcagca acggcggcac
     3541 gtcgggcaga tgcaggccgt gggcctctgc catcgtgttc cgtacgattc tggcttgcgc
     3601 ttcttgcaac ggccaggctt tgtggcggat ctcggcttta tacaggcggc ccccgctatc
     3661 ctgcgcgaat agccggaacc gggcgctcag ccagtgctcc aggctgccgg ggcgggcgtt
     3721 aaacacgccg gataccggct cgtagcccgc gacgaagacc gcgccgggcg cgccgggatg
     3781 ccgccgccgc gacacatagc ggatgcgccg gcccgcgcgc gtgtgggtcg cctgcgtatt
     3841 gaaatagggc aggtgataga cgatgcgcgc gcccagcacg gccaggacgt tggcggcgtc
     3901 aaggctcagg aacagcacgc ccgagccgcc gggccctcgc acgtacgtac gcacgttgat
     3961 ctcgtggaag cgcgcggtgc cggggatcga cggcatgccg cgggcacgca ggtggctgat
     4021 ccagaagggc gtgatcccga gccatgcctg tccgtcgaac gtatcgagtg tcagcccgcg
     4081 cggcacgcat ggggtcagcg cgtccggcgg cagcggccag tgggcgaata gcaggtcgtt
     4141 ccacgtctgc gtcatgaccc acgcccccgg cagacccgcc ggcgtggtca cgcgcgggat
     4201 tacattatgg tcgcggcttg cctccatcat gataattccc aatcgatgtc tggttcccag
     4261 agctcacata catggatctg cagctcgctg ccggcgacac aggcgcggca cactcacgcg
     4321 gactagcatt ttctacgccg cgtttcgggt cctgtcgtgc cggctgccgg caactcgctt
     4381 tcgacacagt tgggcttgcc ggcgtccgcc tgcccatcgt ggctacgtct ctggaccgag
     4441 gcatgtccgg gacaaaggtg aaaccggact ccttcacgtg cctgatgtac attggtcgga
     4501 gttgctctat actggtgaca gttgacga
//
LOCUS       DASUYC010000754         3356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_810388,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000754 DASUYC010000000
VERSION     DASUYC010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810388"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..1424
                     /locus_tag="VF276_12420"
     CDS             171..1424
                     /locus_tag="VF276_12420"
                     /inference="COORDINATES: protein motif:HMM:NF012964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX6607710.1"
                     /translation="MAFIPLVITVVMGIMAGGMVALALIGPRHIQNIFGILALFGVVL
                     GLAAAVIGQPLWPTLINNLLDLILSGFIGYAVTTYFVLSYQPKPEINPPAPGEAQTAQ
                     RDGPGKTAVIYFAPGESAQYDGRIAARGFDTIDYPEALPPTLLRPLYLNDLKRKYEAV
                     GASPHRAIHLKLAQKVQDRLGKRYKVYIAFYNDHPSLTEVAAEAVREGARRLIILHVR
                     LTSPPPSVKPEEWLDAALRLKRYGVRVVETAPLWNSDLLPRVFVRRALAATEGLDRAT
                     VGLMLIGQGHPLPRRAVPEAKDVPPPLQRQNQELTFQKRVRQALIRAGFPDDQVVPAW
                     LQWQHPTIEGAYAQLVAESCRRIFWIGTGLMTDSMVTLYDIPAILRPAAERSGVEVTA
                     LGPWNDDDVVAEALVERVKQVALGD"
     gene            complement(1510..1638)
                     /locus_tag="VF276_12425"
     CDS             complement(1510..1638)
                     /locus_tag="VF276_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607711.1"
                     /translation="MNTNDYLLSQMSRQIHAERVRAAQDAHLARLAAAFRRLRPSK"
     gene            complement(1854..2030)
                     /locus_tag="VF276_12430"
     CDS             complement(1854..2030)
                     /locus_tag="VF276_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607712.1"
                     /translation="MSPNEFMLSQLSREIVADRLREAEQARSARLIAALRRLQPRKRQ
                     SPPGPAPAPRKAPV"
     gene            complement(2171..2350)
                     /locus_tag="VF276_12435"
     CDS             complement(2171..2350)
                     /locus_tag="VF276_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607713.1"
                     /translation="MITHEYLLDRLSREITAERVQEAKNARMARIVAAVRRENAGSGA
                     KHQSLRRETASKAAA"
     gene            2645..3328
                     /locus_tag="VF276_12440"
     CDS             2645..3328
                     /locus_tag="VF276_12440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEX6607714.1"
                     /translation="MTQQPAGAAVAPALPKPLLRGRIHAAAAVGAVIVTVALCWRSRN
                     DLPRLISMLIFGLSMIELYTVSAVYHMGHWRPQVQRVLRSVDHANIFVLIAGTYTPLC
                     FNVLTDWVRTTILSLIWVLAAAGIGLSVFTPRVPRWVGTGLYIGMGWVAILALPAFFA
                     VLPVEAIALMVLGGVLYTLGAVVYGRKRPNPFPRVLGFHEIFHLFTVAAGAVFALVIW
                     IWVVPFPRV"
BASE COUNT          488 a         1149 c         1138 g          581 t
ORIGIN      
        1 caggcggcct tcgtgggcat agccggcggc ttatggggta tagcccggcc gggcgcgcgg
       61 tgcgggccgg ggcgaaacat gctataatag ccgcgaccgg cgcggcacac gcgccgggcg
      121 gtcccggacg cccggctctc cgccgccggg actcgcggca ggagggtcgc ttggcgttta
      181 taccgctcgt catcacggtt gtcatgggca tcatggccgg gggcatggtc gcgctcgcgc
      241 tcattggtcc ccgccacata cagaatatct tcggcatcct ggccctgttc ggggtggtgc
      301 tggggctggc cgccgccgtc atcggccagc cgctctggcc cacgctgatc aacaacctcc
      361 tcgacctgat cctctccggc tttatcggct atgccgtcac gacctacttc gtgctctcct
      421 atcagccgaa gccggagatc aacccgcccg cgccgggcga agcccagacg gcccagcgcg
      481 acgggccggg caagaccgcc gtgatctatt tcgcgcccgg cgagtccgcc caatacgacg
      541 ggcgcatcgc agcgcgcggc ttcgacacaa tcgactatcc cgaagcgttg ccgcccacgc
      601 tgctgcgccc gctctatctg aacgacctca aacgcaaata tgaagccgtc ggcgccagcc
      661 cgcaccgggc catccacttg aagctggcgc aaaaggtgca agatcggctg ggcaaacgct
      721 acaaggtcta catcgccttc tataacgacc atcccagcct caccgaggtg gccgccgagg
      781 ccgtgcgcga gggggcgcgc cgcctgatca tcctccacgt ccggctgacc agccctccgc
      841 cgagcgtcaa gcccgaagaa tggctcgatg cggcgctgcg cctgaagcgt tacggggtgc
      901 gcgtcgtcga gacggcgccg ctgtggaact cggacctact gccgcgcgtg ttcgtgcgcc
      961 gggcgctggc cgccaccgaa ggcctcgacc gggccaccgt ggggctgatg ttgatcggcc
     1021 agggccatcc cctgccgcgg cgcgcggtgc ccgaagcaaa ggacgtgccg ccccccttgc
     1081 aacgccagaa ccaggaactg acctttcaga aacgggtgcg ccaggcgctg atccgggccg
     1141 gcttccccga cgatcaggtc gtgcccgcct ggctgcaatg gcagcatccc accatcgagg
     1201 gcgcctatgc ccaactggtg gccgagagtt gccggcgcat cttctggatc ggcaccggcc
     1261 tgatgaccga cagtatggtg actctctacg acatcccggc cattctgcgg cccgccgccg
     1321 agcgcagcgg cgtcgaagtc acggcgctgg gcccctggaa cgacgacgac gtggtggccg
     1381 aggccctggt cgagcgcgtc aagcaggtgg ctctcggcga ctgaccacta gtccagcgca
     1441 cggcagacac agcaacgccc cgccggtccc ccatgggggc acgccgcggg gcgttccgat
     1501 gctgattgct tacttgctgg ggcgcagccg gcggaaggcg gcagcgaggc gggccaggtg
     1561 ggcatcctgc gcggcgcgca cgcgctctgc gtgaatctgg cggctcatct ggctcagcaa
     1621 atagtcattc gtgttcatcg tggtgtcctt tctcctcaga tgtgttatgt tctgtgtttc
     1681 gttaccgttc tatcttagca ccggcgcgct gccggcacat cgggacggcg cggtatccgc
     1741 cgctaccaca ttcgcctgcc gggcatcggt cgccaggatc ggtgcccgga tcggtctgca
     1801 gaccgagcga tccaggcgcg ggcgggcggg cggcctgcga ccctggtggg aggctagacc
     1861 ggcgctttgc gcggcgcggg ggccgggccg ggaggcgact gccgtttgcg cggctgcagc
     1921 cggcgcaggg cggcgatgag gcgggcgctg cgggcctgct cggcttcgcg cagacgatcc
     1981 gcgacgattt cccggctcag ttgcgacaac atgaattcat tggggctcat ggccgtttcc
     2041 tttcttccaa ggccggtcgc gccaccgatc ggggtcgcga ccgctcacga tagacatgca
     2101 aaggcccgtc cggcggggaa gcctgggtag acttcccagg ctggacgggc ctgagataag
     2161 cgaagaacgg ttaagcggcg gctttggaag cagtttcgcg gcgaaggctt tggtgcttgg
     2221 cccccgatcc ggcgttctcc cggcggacgg cggcgacgat ccgcgccatg cgtgcgttct
     2281 tggcttcctg gacccgctcg gccgtgatct cgcgactcag gcggtctaag agatactcgt
     2341 gcgtaatcat ctccgtatcc tttctccggg ttctccggtc gtgtgtgccg gcctggggtc
     2401 gacaccgtgt tgttgatggt gtaatcttac ctggatacgg gaattcgcgc atcggcgggg
     2461 ggtggtctcc cccggccccg cgattgtccg ggcgggtaac ggaccgggag acccggcccg
     2521 cctacatctg cgggcggagc gcccctggac ccgaggcgtg gcccatcccc caccctggac
     2581 tggcagccgc gcccgatcct ggagagccgg cccctgagaa ccgggccggc acaaggagta
     2641 atcgatgacc caacaaccag ccggcgcggc agtcgcgccg gccctgccca agccgctgct
     2701 gcgcggccgc atccacgccg cggcggccgt gggcgccgtg atcgtgactg tggcgctgtg
     2761 ctggcgcagc cgcaacgacc tgccgcgcct gatctcgatg ctcatcttcg gcctgagcat
     2821 gatcgaactc tacacggtca gcgcggtcta ccacatgggc cactggcgac cccaggtcca
     2881 gcgcgtcctg cgctcggtgg accacgccaa catcttcgtg ctgatcgcgg ggacgtatac
     2941 gccgctctgc ttcaacgtgc tgaccgactg ggtgcgcacg acgatcttga gtttgatctg
     3001 ggtgctggcg gcggcgggga tcgggctctc ggtgttcacg ccgcgcgtgc cgcgctgggt
     3061 gggcacgggc ctctacattg gcatgggctg ggtcgccatc ctggcactgc cggccttctt
     3121 tgccgtgctg ccggtggaag ccatcgcgct gatggtgctg ggcggggtgc tctacacgct
     3181 gggcgcggtg gtctacgggc gcaagcggcc caacccgttc ccccgcgtgc tgggcttcca
     3241 cgagatcttc cacctgttca ccgtggcggc aggggcggtc ttcgccctgg tgatctggat
     3301 ctgggtggtg cccttcccgc gcgtctagcg ggcaataaaa acgcccgccc ggcccc
//
LOCUS       DASUYC010000755         2399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1270340, whole genome shotgun sequence.
ACCESSION   DASUYC010000755 DASUYC010000000
VERSION     DASUYC010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270340"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..1405
                     /locus_tag="VF276_12445"
     CDS             221..1405
                     /locus_tag="VF276_12445"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX6607715.1"
                     /translation="MDAEHSYLTHLECAECARTWPAGEEQHRCACGGILLARYDLAAL
                     ARDVPRDTVAARPWGAGLWRYAELLPVADPAARVSLGEGTTPLLPLPAFGAELDITLW
                     MKDESLNPTGTFKARGAAVGVSRARELGARTIALPTAGNAGAAWAAYGARAGLPVVVA
                     MPCDAPRLTQQEVQRYGARLHLVDGLISDAGTWVAQGVQAEGWYDASTFKEPYRLEGK
                     KTLGLEIAEQLDWQPPDVILYPTGGGVGLIGLWKAFQELRALGWIAADRPLPRLVVVQ
                     AAGCAPVVRGWDSGTDSTSFWEGAHTVAAGLRVPGPLAGGLMLRALRETKGTALTVDD
                     DTLLAALGELSRAGLWVCPEGATLLPAVRRLRAAGWIKAGERVVLLNTGAGMVYPEVG
                     AV"
     gene            complement(1466..>2399)
                     /locus_tag="VF276_12450"
     CDS             complement(1466..>2399)
                     /locus_tag="VF276_12450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX6607716.1"
                     /translation="WRWRHLLAPLMAPAPGLPALFGMTMTGYFFNTFFPARAGDLMRA
                     HLLSRRSGLRRTTILATVVIEKLFDGMALLVLLVAGIPALSIGEARTEQLGLVAVLVL
                     GLALGGLLVFHWQAERVVALTARLVDVLPVPERIGSLAVRLVHTFSAGLRVFEKPGPL
                     FKSAGISLLVWITAAGMFWAAMAAFQIAIPSPSALLLMTAVVNLGLLIPAMPGNVGNY
                     EALILTALVLILPGIDKDMAVAFALVFHAAQLVATLLVGGIAFWSQRVSMREMQADAG
                     EAVAAAPADSADRLREHVHHTPREEASELGIEKL"
BASE COUNT          367 a          765 c          929 g          338 t
ORIGIN      
        1 ctcttcgctg cgctcagagt gacaagcatg ctgtgacaga ctacgagact cttcgctgcg
       61 ctcagagtga caaacacgct gcgacagact atcaccttgt ttctagcgcc ggcagggcat
      121 tatctgggca cggtgcaccg gcggcttgtc gaagggtggt ggcttagggc gggcgggggc
      181 gggctgatgt gttatacttg gtaaggtaag ggggatgact atggatgccg agcatagcta
      241 tctaacacac ctggagtgcg ccgagtgcgc gcgcacctgg cccgccgggg aggagcagca
      301 ccgctgcgcc tgcgggggga tcttgctggc gcgctacgat ctggcggccc tggcgcggga
      361 tgtcccgcgc gacaccgtgg cggcgcggcc ctggggcgcg gggctgtggc gctatgccga
      421 gttgctgccc gtggccgatc cggcggcgcg cgtgtcgctg ggcgagggca cgacgccgtt
      481 gctgcccctg ccggcgttcg gcgcggagct cgatattacg ctgtggatga aggacgagag
      541 cctgaacccg accggcactt tcaaagcgcg cggcgcggcg gtgggcgtat cgcgagcgcg
      601 agaactgggc gcgcgcacca tcgcgctgcc cacggcgggg aatgccgggg cggcctgggc
      661 ggcctacggg gcgcgggctg ggctgccggt ggtggtagcg atgccgtgcg acgcgccgcg
      721 gctcacgcag caggaggtgc aacgctacgg cgcccggctg cacctggtgg acggcctgat
      781 cagcgacgcc gggacctggg tggcccaggg ggtgcaggcc gagggctggt acgatgccag
      841 caccttcaag gagccgtacc gtctggaggg caagaagacg ctgggcctgg agatcgccga
      901 gcaactcgac tggcagccgc ccgacgtgat cctctatccc acgggcggcg gcgtggggct
      961 gatcggcctc tggaaggctt tccaggaact gcgcgcgctg ggctggatcg cggcggaccg
     1021 gccactgccg cggctggtgg tggtgcaggc ggcgggctgc gcgccggtgg tgcgcggctg
     1081 ggatagcggc accgactcga ccagcttctg ggagggcgca cacacggtgg ccgccgggct
     1141 gcgcgtgccg ggcccgctgg ccggcgggct gatgctgcgc gcgctgcgcg agacgaaggg
     1201 cacggcgctg acggtggacg acgacacctt gctggcggcg ctgggcgaac tgagccgcgc
     1261 cgggctgtgg gtctgcccgg aaggggccac gttgctgccc gccgtgcgcc gcctgcgcgc
     1321 cgccgggtgg atcaaggccg gcgagcgcgt ggtgttgctg aacaccggcg cgggtatggt
     1381 atatcccgag gtaggggcgg tttaggcgcc aggcgggggc cggtttcaaa ccggccccta
     1441 caccgactgg ccgggcgcga gccggttata gcttttcgat gccgagttcg gaggcttctt
     1501 cgcgcggggt gtggtggaca tgctcgcgca ggcggtcggc gctgtcggca ggcgcggcgg
     1561 cgacggcttc gcccgcatcg gcctgcatct cgcgcatgga aacgcgctgg ctccagaagg
     1621 cgatgccgcc gaccagcagg gtcgcgacca gttgcgcggc gtggaagacc agggcaaagg
     1681 ccacggccat gtctttgtcg atgcccggca ggatcagcac cagcgcggtc aggatcagcg
     1741 cctcatagtt gccgacgttg cccggcatgg ccgggatcag caggcccagg ttgaccacgg
     1801 cggtcatcag cagcagcgcg ctggggctgg ggatggcgat ctggaaggcg gccatcgccg
     1861 cccagaacat gcccgccgcc gtgatccaga cgagcagcga aataccggcg ctcttgaaca
     1921 gggggccggg cttctcgaac acgcgcaacc cggcgctgaa cgtgtgcacc aggcgcaccg
     1981 ccaggctgcc gatccgctca ggcaccggca gcacgtcgac caggcgcgcg gtgagcgcga
     2041 cgacgcgctc ggcctgccag tggaagacga gcaagccgcc cagcgccaga ccgagcacca
     2101 gcaccgctac cagccccaac tgctccgtgc gggcctcgcc gatgctcagc gcggggatgc
     2161 ctgctaccag caacaccagc agggccatcc cgtcgaacag cttctcgatg accaccgtgg
     2221 caaggatcgt cgtgcggcgc aggccgctgc gccggctcag caggtgggcg cgcatcaggt
     2281 cgccggcccg cgccgggaag aaggtgttga agaaatagcc cgtcatggtc atgccgaaaa
     2341 gggcgggcag accgggggcc ggggccatga gcggcgccag caggtggcgc cagcgccag
//
LOCUS       DASUYC010000756         2759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_810535,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000756 DASUYC010000000
VERSION     DASUYC010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810535"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..>2759
                     /gene="cphA"
                     /locus_tag="VF276_12455"
     CDS             105..>2759
                     /gene="cphA"
                     /locus_tag="VF276_12455"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX6607717.1"
                     /translation="MQIERIRVLRGPNIWAYRPVFEIWLNIGRFEELPSNKLPGFTER
                     LVALLPSLWQHRCSEGQPGGFLERLRLGTYMGHILEHIILELQSLAGMDAGYGRTRGT
                     GRHGQYRVVVDYKDEEAGRMCVDLALEIVDAITADPPRTIDLKPRLDAIREVAEDNML
                     GPSTQALVDAARKRRIPWFRLNSGSLVQLGHGASARRIQAAETSFTSNVGVEIASDKE
                     LTKQLLGRVGVPVPAGAIVRSVEEAWEAAESVGLPVVVKPKDGNQGKAVSVNLMTREQ
                     VETATRLALEYDRRVIVESYYPGNDYRLLVVNGKLVAAALRRPAQVVGNGHDNIRRLV
                     ELVNEDPRRGVGHGAAMTRIRCDEAAELTLTKQGLTWDDVPAAGQVVLLRDNSNLSTG
                     GTATDVTDEVHPDNCAIAELAARTIGLDIAGVDVVTRNIRHSMLDHGGGIVEVNAAPG
                     LRMHLYPSEGQGRPVGEAVINMLFPPGTQARVPLIAVTGTNGKTTVTRMIAAVYATMH
                     KFVGMTTTDGIYFGGRMSVAGDCSGPRSAEAVLQHPDVEVAVLETARGGILRSGLGWD
                     RCQVAVVTNISNDHLGLDGIDSPERLAEVKQVIVESVMPGGCAVLNAEDRLVARMADA
                     TKERIIFFGTDPRGPVLSRHLAAGGKAVFLRGSMITLAEGAKETLLLDVAEVPATLGG
                     KISFQAVNAMAAAAACWGADCPLDAIRLGLRTFQPDTQTAPGRFNLFEIGQAQVILDY
                     GHNPHALRAIQNAVRALAPRKAIGVVAAPGDRRDADIGELARIAAETFDAIIIREDDD
                     LRGREPGEVAGLIRQTIEDYRPDLPLAIIADEAEAIRAAMDMAAPGDLVVAFIDRLQG
                     SIDLVRAYAAEVAAGGSGLACTLGPAPGP"
BASE COUNT          441 a          983 c          943 g          392 t
ORIGIN      
        1 ccgttctatt acgtggtatc ctggcggcac tttacttgcc ggcgccggga gatccggtct
       61 actccgggcg cgtcctgccg ggcggcgccc cgaaggagtc ttgtatgcag atcgagcgca
      121 tccgcgtgtt gcgcgggccg aatatctggg cctaccggcc ggtttttgag atctggctca
      181 atatcgggcg cttcgaggag ttgccctcca acaagctgcc cggcttcact gagcggctgg
      241 tcgcgttgct gcccagcctc tggcaacacc gctgctccga gggccagccc ggcggcttcc
      301 tcgaacgctt gcgcctgggc acctacatgg gccatatcct cgaacacatc atcctggaat
      361 tgcagtcgct ggcgggcatg gacgccggct acgggcggac gcgcggcacc ggccgccacg
      421 ggcaatatcg cgtggtggtc gattacaagg acgaggaagc gggccgcatg tgcgtggacc
      481 tggcgctgga aatcgtcgac gcgatcaccg cggatccgcc ccgcactatc gacctcaagc
      541 cccgcctcga cgccatccgc gaggtggccg aagacaacat gctcggtccc agcacccagg
      601 cgctggttga tgcggcccgc aagcggcgca tcccctggtt ccgtctgaac agcggcagcc
      661 tggtccagtt gggccacggc gcctcggccc ggcgcatcca ggccgccgag acctcgttca
      721 cgtcgaacgt cggcgtcgag atcgcctcgg acaaagagct gaccaagcag ttgctgggcc
      781 gggtgggcgt gccggtgccc gcgggcgcca ttgtccgcag cgtcgaagaa gcctgggagg
      841 cggcggagtc ggtgggcctg cccgtcgtcg tcaagcccaa ggacggcaac cagggcaagg
      901 ccgtgagcgt gaacctgatg acccgcgagc aggtggagac ggccacccgg ctggccctgg
      961 agtacgaccg gcgggtcatc gtcgaaagct actaccccgg caacgactac cgcttgctgg
     1021 tggtcaacgg caagttggtc gccgccgctc tgcgccgtcc cgcccaggtc gtcggcaacg
     1081 gccacgacaa tatccgccgg ctggtcgaac tcgtcaacga ggacccccgg cgcggtgtcg
     1141 ggcacggcgc ggccatgacc cgcatccgct gtgacgaagc cgctgaactg acgctgacca
     1201 agcaggggct gacctgggac gacgtgcccg cggcgggcca ggtggtgttg ctgcgcgaca
     1261 acagcaacct cagcaccggc ggcaccgcca ccgacgtgac tgacgaggtt caccctgata
     1321 actgcgccat cgccgaactc gccgcgcgca ccatcggcct ggacatcgcc ggggtggacg
     1381 tggtcacgcg caacatccgc cactcgatgc tggaccacgg cggcggcatc gtcgaagtga
     1441 acgcggcgcc gggcctgcgc atgcacctgt acccgtcgga gggccagggc cggcccgtgg
     1501 gcgaagccgt catcaatatg ctcttcccgc cgggcaccca ggcccgcgtg cccctgatcg
     1561 ccgtcaccgg caccaacggc aagacgaccg tcacgcgcat gatcgccgcc gtctacgcga
     1621 cgatgcacaa gttcgtgggc atgacaacca cggacggcat ctatttcggg ggccgcatga
     1681 gcgtggccgg cgattgcagc gggccgcgca gcgcggaagc cgtgttgcag cacccggacg
     1741 tggaggtggc cgtgctcgaa acggcgcgcg gcggcatctt gcgctccggc ctcggctggg
     1801 accgctgcca ggtcgccgtc gtcaccaaca tcagcaacga ccacctgggc ctggacggca
     1861 tcgacagccc cgagcggctg gccgaggtca agcaggtgat cgtggagagc gtgatgcccg
     1921 gcggctgcgc cgtgctgaac gcggaggacc ggctggtggc gcgcatggcc gacgcgacca
     1981 aggaacggat catcttcttc ggcaccgacc cgcgcggccc cgtcctgtcg cgccacctgg
     2041 cggcgggcgg caaggccgtg ttcttgcgcg gcagcatgat cacgctggcc gaaggggcga
     2101 aggaaacgct gctgctcgac gtggccgagg tgcccgccac cctgggcggc aaaatcagct
     2161 tccaggcggt caacgccatg gccgccgccg ccgcctgctg gggcgccgac tgcccgctcg
     2221 acgccattcg cctgggcctg cgcacgttcc agcccgatac gcagacggcg ccgggccgct
     2281 tcaacctctt cgagatcggc caggcccagg tcatcctcga ctacggccac aacccgcacg
     2341 ccctgcgcgc catccagaac gccgtacgcg ccctggcccc gcgcaaggct atcggcgtcg
     2401 tggccgcgcc cggcgaccgc cgcgacgccg acatcggcga gctggcacgc atcgccgccg
     2461 agaccttcga cgccatcatc atccgcgagg acgatgacct gcgcgggcgc gaaccgggcg
     2521 aggtggccgg cctgatccgc cagacaatcg aggactaccg tcccgacctg ccgctggcga
     2581 ttatcgccga cgaggcggag gcaatccggg cggcgatgga catggccgcg ccgggcgacc
     2641 tggtggtggc ctttatcgac cgcctccagg gcagcatcga cctggtgcgc gcctacgccg
     2701 ccgaggtggc cgccggaggc agtgggctgg cctgcacgct cggtcccgcg cccggcccg
//
LOCUS       DASUYC010000757         2827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_435665,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000757 DASUYC010000000
VERSION     DASUYC010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435665"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VF276_12460"
     CDS             complement(<1..518)
                     /locus_tag="VF276_12460"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6607718.1"
                     /translation="MAPHERAAVGAIIAVAFADKYGPALGNDPALAAEVAAVLPSGWA
                     CYVGEQAGVVYGAGLLRFAEHPGFGTAEALAMWRVLRRRQSLGRTLRSLVLLSLTGAD
                     HRPDARTGYISALAVLPARQGQGLGSGLLRHLEAEARAAGKTRLTLDVVDTNTGARRL
                     YERHGFRIIETQP"
     gene            complement(544..1578)
                     /locus_tag="VF276_12465"
     CDS             complement(544..1578)
                     /locus_tag="VF276_12465"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEX6607719.1"
                     /translation="MAVMSKKDRINATMRNEPVDRIPVSLWRHFYECEETAEGLAGAM
                     LAWQAKYDWDWMKVNPRASYHVEGWGVKLRFSGQPLVKPVVTEVPIHSVQDWGKLRPL
                     PLDSGPLDEQLEVVERLCEALGDKVYAVETVFNPISIAADLLPEGRDLLTHIQQDPQA
                     VHGALEVITATFTEFVGRLLKAGADGIFFATTDWASRDMLTEDQYAEFGRPYDLRVLA
                     AAQDAHFNVLHVCKNNNYVLSMLDYPVHAINWAVGSPGNPDLRTVQTSTDKVVIGGYR
                     NETLRDSDVAHIHAEARAADLQTGGRRWILGPACSIAVDTPDANVRAARDIANQLDTG
                     AAGGGQDITG"
     gene            1769..2776
                     /locus_tag="VF276_12470"
     CDS             1769..2776
                     /locus_tag="VF276_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607720.1"
                     /translation="MYRIFGSHRRRRAAALLALAALLLIPLAAPPARAADFPTNPVAD
                     PHQAGVRWFAATGHTLRGTFLAYWEQHGGLAQFGYPITEEFTESGPTANKPVVVQYFE
                     RNRFEHHPENAGTPYEVLLGLLGQEFHAPDPKVAPQTGPATQYFEATGHNLGGAFLRY
                     WQTHGGLAVHGYPISEPFQETNPIDGKQYTVQYFQRSRFELHPENAGTAYEVLLGLLG
                     TQLARQKGYFPPATPPAVFPRFGHAADFSWIAGQINVTRIQGGCTYLRYEDKPAGVGN
                     GQVDPTGPVWTTALNAGLAPNNAYVVVYGHMAGPDEPHEMCFAPMYVVDKVEPNPAGA
                     P"
BASE COUNT          455 a          974 c          960 g          438 t
ORIGIN      
        1 ggctgcgttt cgatgatgcg gaagccgtgg cgctcgtaga ggcggcgcgc gcccgtgttg
       61 gtatcgacta catccagggt caggcgggtt ttgccggcgg cgcgggcctc agcttccagg
      121 tggcgcagca ggccgctgcc cagcccctgg ccctgccggg cgggcagcac cgccagcgcc
      181 gagatgtagc cggtgcgcgc gtcgggccgg tggtcggcgc ccgtcagcga cagcaggacc
      241 agcgagcgca gtgtgcgccc cagggattgg cggcgccgca gcacgcgcca catcgccagc
      301 gcctcggcgg tgccgaagcc gggatgctcg gcgaagcgca acaggccggc gccatacacg
      361 acgccggcct gctcgcccac ataacacgcc catccggagg ggagcaccgc ggcgacctcg
      421 gcagccagcg ccgggtcgtt gcctagtgcc ggcccatatt tatccgcgaa agccacggcg
      481 atgatcgccc cgacggcggc ccgctcatgc ggggccatgc gccggaacgt ccacggagac
      541 ggatcatccg gtgatatcct gcccaccccc tgccgccccg gtgtccaact ggttggcgat
      601 gtcgcgcgcc gcgcggacgt tggcgtcggg cgtatccacg gcgatggagc acgccggacc
      661 gaggatccag cggcggcccc ccgtctgcaa atcggcggcg cgggcttcgg cgtggatatg
      721 ggccacatcg ctgtcgcgca aggtttcgtt gcggtacccg ccgatgacca ccttgtcggt
      781 gctggtctgc acggtgcgca ggtcggggtt gcccggcgag ccgacggccc agttgatcgc
      841 gtgcaccggg tagtcgagca tcgagagcac atagttgttg ttcttgcaga cgtggagcac
      901 gttaaagtgc gcgtcctgcg ccgccgccag cacgcgcagg tcatagggcc gcccaaactc
      961 cgcgtactgg tcctccgtca gcatatcgcg cgaggcccag tcggtggtgg cgaagaagat
     1021 gccgtcggcg cccgccttga gcaggcggcc cacgaactcg gtaaacgtgg cggtgatcac
     1081 ttccagcgca ccgtgcacgg cctgggggtc ctgctggata tgggtgagca ggtcgcgccc
     1141 ctcgggcagc aggtcggcgg cgatggaaat cgggttgaac accgtctcca cagcgtagac
     1201 cttatcgccc agcgcctcgc agagccgttc caccacctcc agttgctcgt ccagcggccc
     1261 ggaatcgagc ggcagcgggc gcaacttgcc ccagtcctgc acggagtgta tcggcacctc
     1321 ggtgacgacg ggcttgacga ggggctggcc gctgaagcgc agctttacac cccacccctc
     1381 gacgtggtag gatgcgcggg gattaacctt catccagtcc caatcatatt tggcctgcca
     1441 ggcgagcatg gctccggcca ggccctcggc ggtttcttcg cactcgtaga agtggcgcca
     1501 gaggctgacg gggatgcggt cgaccggctc gttgcgcatc gtggcgttga tgcggtcttt
     1561 cttgctcata acagccatgg gaatcgtggc tcctttcgtg cgtggactac gcaggctacg
     1621 gcgccagcag gatctatacc acacctgccg gcgggcgcgg tggggaactc gcggcgagca
     1681 ggagcgttat ggtatacagg ggcgcgcgtc gcggggcgag cagctatccg gctgccggcc
     1741 cggcgacttc aacagaagga gggacttgat gtacaggata tttggctcac atagacggcg
     1801 ccgcgcggcg gccttgctgg ctctggcggc actccttctc atccccctgg cggcgccacc
     1861 ggccagggcc gcggactttc ccaccaaccc cgtcgccgat ccgcaccagg cgggcgtgcg
     1921 ctggttcgcc gccaccggcc acaccctgcg cggcaccttc ctcgcctact gggaacagca
     1981 cggcgggttg gctcagttcg gctatccgat caccgaagag ttcaccgaat ccggccccac
     2041 ggccaacaag ccggtcgtcg tccagtattt cgagcgcaac cgcttcgagc accacccgga
     2101 gaacgcgggc acgccctacg aagtgctgct ggggttgctg ggccaggagt tccacgcgcc
     2161 cgatccgaag gtcgcgccgc aaaccggccc ggccacccaa tacttcgagg ccaccgggca
     2221 caacctcggc ggcgcgttcc tgcgctactg gcagacgcac ggcggcctgg ccgtccacgg
     2281 ctacccgatc agcgagccgt tccaggaaac caaccccatc gacggcaagc agtacacggt
     2341 gcagtatttc cagcggtcgc gcttcgagtt gcacccggag aacgcgggca cggcatacga
     2401 agtgctgtta gggttgctgg gcacgcaact ggcgcggcaa aagggctatt tcccgcccgc
     2461 cacgccgccc gcggtgttcc cgcgcttcgg ccatgccgcc gatttctcct ggatcgccgg
     2521 gcagatcaac gtgacccgca tccagggcgg ctgcacgtac ctgcgctatg aggacaagcc
     2581 cgcgggcgtg ggcaacggtc aggtggaccc gaccggcccc gtgtggacca cggcgctgaa
     2641 cgccgggctg gcgcccaata acgcgtatgt cgtcgtctac ggccacatgg ccggcccgga
     2701 cgagccgcac gagatgtgct tcgcgccgat gtacgtggtc gataaagtcg aaccgaaccc
     2761 ggcgggcgcg ccctgacaga cggcccggcg ctgcccgcct gctgcgggca gcccggcccg
     2821 aaaggag
//
LOCUS       DASUYC010000758         2474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1966296, whole genome shotgun sequence.
ACCESSION   DASUYC010000758 DASUYC010000000
VERSION     DASUYC010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1966296"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="VF276_12475"
     CDS             <1..1005
                     /locus_tag="VF276_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607721.1"
                     /translation="GWSYSANGGQGGGSACGSAPVPARIDQVFHFLRVSVNGTQVTVA
                     PTDSRGRTFDVQTYNFGSGAATATRTPTSTRTPVVTATQTSTRTPLPTRTATPPPTAA
                     GTATTSPATATPAVTPPTVPATATAGSTPSATASATRTATPPATPAAVFSDGFETGDL
                     AAWTSSSGLSVQRTLVHAGSYAAQANTTNGTTYAKKTLPSEYGEGYARLYVNLISTSS
                     QVNLVRYRTAGDGSLGYLFVSPTGQLGLRNDVAGVTLTSATALGSGWHTLEFHALIAG
                     AASRTDVWLDGSLVSALSGTTNFGTSPIGRLQVGEVQSGRTYNVVFDDVVFATQRSGP
                     "
     gene            complement(1031..1777)
                     /locus_tag="VF276_12480"
     CDS             complement(1031..1777)
                     /locus_tag="VF276_12480"
                     /inference="COORDINATES: protein motif:HMM:NF021465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HEX6607722.1"
                     /translation="MSLVQLRGTGAPGQSLRRFFWTHPEWWTLVLVALAWGTIVAPAG
                     GTQEHMRHTMPAPDPMPFQEEWLHWGLMVGAMMVPLMLEPLRWVAFQSYRHRRHRAIL
                     LFLIGFLLPWMVIGIGVAALRTLDWSRNPLLAVGTFGLAALWVLVPVRMRALVFCHLR
                     LPLAPAGWKADRDCLRFSVLVGASCAGVCGLLMLARALTGHNLIAMLGGTVLGALEYR
                     AFRPPTRPIVAGTLLLAAWFLLPISSALPP"
     gene            complement(1774..>2474)
                     /locus_tag="VF276_12485"
     CDS             complement(1774..>2474)
                     /locus_tag="VF276_12485"
                     /inference="COORDINATES: protein motif:HMM:NF023564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607723.1"
                     /translation="QWLSRTAFSLHDATAQIVTLRCADVVDTSALDYTYDDVSDPLAV
                     PRPAARLAPRVPPTEVTMPDSIPPELVGATETSVQVGNTPARIRVPTPAHATARKRLA
                     AAAEAAAPAESIAPAPAAPPRVLLQLENLTAGGRANSYDVYVNIPAGDDPTQHPDRFA
                     GRLDLFGVAEASRATAQHPGSGLTYTLDISGIYHELSTAPGWDPEHIDVSLVPVRKWE
                     GAPVTVGRVSLFFG"
BASE COUNT          459 a          838 c          860 g          317 t
ORIGIN      
        1 ggctggagtt acagcgcgaa cgggggccag ggcggcggca gcgcctgcgg gagcgcgccg
       61 gtcccggcgc ggatcgacca ggtcttccat ttcctgcgcg tgagcgtgaa cggcacgcaa
      121 gtgaccgtgg cccccaccga ctcgcggggc cgcaccttcg acgtacagac atataacttc
      181 ggcagcggcg cggcaacggc gacgcggacg ccgacgagca cccggacacc agtggttacc
      241 gcgacccaga cctcgacccg cacaccccta cccacccgga cggcgacgcc acctcccacc
      301 gcggccggca cggcaaccac gtccccggcg acggctacgc cggcggtgac cccgccgacc
      361 gtgccggcga cggccacggc cggcagcacg ccgagcgcga ccgccagtgc gacccgcacg
      421 gcgaccccgc cggcaacacc cgcggcggtg ttcagcgatg gattcgagac aggcgatctg
      481 gccgcctgga ccagcagcag cgggctgagc gtgcagcgga cgctggtgca cgcgggcagc
      541 tacgcggcgc aggccaacac gaccaacggc accacgtatg ccaagaaaac cctgccgagc
      601 gaatatgggg aagggtacgc ccgcctctac gtgaatctaa tcagcactag cagccaggtc
      661 aacctggtgc gctatcgcac ggccggggat ggctcactgg gctacctgtt tgtcagtccg
      721 accggccagt tagggttgcg caacgatgtg gcgggcgtca ccctcaccag cgcaaccgcc
      781 ctcggtagtg ggtggcacac cctggagttc cacgcgctga tcgccggcgc ggcgagccgc
      841 accgatgtct ggctggacgg gagcctggtg agcgcgctat caggcaccac aaacttcggc
      901 accagcccga tcgggcggct gcaggtgggt gaggtgcaga gcgggcgcac ttacaatgtt
      961 gtcttcgatg atgtcgtctt cgcgacccag cgcagcgggc cctgagcggg atttatggcg
     1021 gaggtagcgc tcaaggtggc agggctgaac tgatcggcag gagaaaccag gccgccagca
     1081 gcagcgtccc cgcaacgatc gggcgggtcg gcgggcggaa agcgcgatac tccagcgccc
     1141 cgagcaccgt gccgccgagc atggcgataa ggttatggcc ggtcagggcg cgggccagca
     1201 tcaacaagcc gcagacgccg gcgcaggacg cgccgacgag gacactaaat cggagacagt
     1261 cgcgatccgc cttccagccc gccggcgcga gcggtagccg caggtggcag aacaccagcg
     1321 cccgcatccg gaccgggacg agcacccaga gcgccgccag cccgaatgtc cccactgcga
     1381 ggagcgggtt acggctccag tcgagggtgc ggagcgccgc gaccccgatc ccaatcacca
     1441 tccacggcag gagaaagcca ataaggaaga gcagaatggc gcggtgccgc cggtgccgat
     1501 agctctggaa agccacccag cgcaacggct ccagcatcag gggcaccatc atcgccccca
     1561 ccatcaggcc ccagtggagc cattcctcct ggaagggcat ggggtccggg gcgggcatgg
     1621 tgtggcgcat atgttcctgg gttccgccgg cgggcgctac gatcgtgccc caggccaggg
     1681 ccaccagcac tagagtccac cactccgggt gcgtccagaa gaatcggcgc agcgattgac
     1741 cgggcgcccc cgtgccccgc agttgaacga ggctcatccg aagaacaggc tcacacgccc
     1801 gacagtcacc ggggcccctt cccatttgcg cacgggcacc agggacacat caatatgttc
     1861 cggatcccag ccgggtgccg tggaaagctc atggtagatg cccgatatat cgagggtgta
     1921 ggtcagaccc gaaccggggt gctgggccgt ggcgcgactg gcttcggcga ccccaaacaa
     1981 gtccagccgc ccggcgaacc ggtccggatg ctgggtagga tcgtctcccg ccgggatgtt
     2041 cacatacaca tcgtaggaat tggcccggcc gccggccgtc aggttctcca actggagcag
     2101 gacgcgcggt ggggcggcgg gcgccggcgc gatcgattcg gcgggcgcgg cagcctccgc
     2161 cgcggcggca agccgcttgc gggccgtggc gtgcgccggg gtgggcacgc ggatacgggc
     2221 cggcgtgttg ccgacctgga cactggtttc cgtggccccg accaattcgg ggggaatgga
     2281 atcgggcatc gtgacctccg tcgggggtac cctgggggcg agccgcgcgg caggccgcgg
     2341 cacggccagc ggatcgctga catcatcata ggtataatca agcgcactgg tatcgacgac
     2401 atcggcgcaa cgcagggtga cgatctgcgc ggtggcatca tgcaaggaaa aggcggttcg
     2461 gctgagccac tgca
//
LOCUS       DASUYC010000759         2873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_395702,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000759 DASUYC010000000
VERSION     DASUYC010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395702"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VF276_12490"
     CDS             <1..344
                     /locus_tag="VF276_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607724.1"
                     /translation="AIAWSAVYNPHWDNDPDAYVAAGVCPWGRTRRLGKLLLPDARFK
                     AYLGVQHVDPRLSGWPGLDAPQARFFVSWFAGGQCLGLRVAPGMAAARDLLWAAWTQQ
                     QPLGAAGGARQ"
     gene            341..772
                     /locus_tag="VF276_12495"
     CDS             341..772
                     /locus_tag="VF276_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607725.1"
                     /translation="MRRSAPAPPWHPITSGWSTVFLLPLAGGLVTAANLLRLGRADKA
                     VFAVLTGLASTAIVLALWSQGPTWANRTFLTLLILSFGDAGLFLYLQGADYRAWADDH
                     PDRGPTSAWVAAIWALLGFMLMIVIWVAWAVLLDLGGPRPR"
     gene            complement(889..2421)
                     /gene="thyX"
                     /locus_tag="VF276_12500"
     CDS             complement(889..2421)
                     /gene="thyX"
                     /locus_tag="VF276_12500"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEX6607726.1"
                     /translation="MTGDTLIYSEHMASGRRSGPTKRTLRELYEMTQTPHGRSRIPLL
                     RLRCLDETTNTFTTGAVRSVVYSGVKPVFRVELEDGKAITCSRDHRFLTPSGWQPLHQ
                     VVGSLTLSPGNIALADTLDTPIATNGQPAYQDYNWLHDHYVVQSLDQATIAELAGVSP
                     HTIRAWVRKLNLQKPLGSWTKGVEPWNKGKQYQAGWHHTAETRVRLSEQKRGDKNPQW
                     RSGITREAITLRRPMKDLRPLVLERDSYRCRLCERVGGRLTVHHILPIWYRPDLRADL
                     DNLVSLCATCHRKVNNHEAEYAERFGVPALQVTELRAAPQPRGRRSVTYKFLRIKSVT
                     YAGEQDTYDIEMAGPHHNFVANGIVTHNSQLSQRYVDESTADFVEPEAIAANPEAHAL
                     FEQAIAAAQEAYVALVELLDKQFVNVPSKTLRRKMARQAARAVLPNATETKIVVTANA
                     RSWRHFIEMRASPYSEPEIRRLAIKILRVLQQEAPNLFGDYEIVTAEDGSEVAVTAHT
                     KV"
     gene            complement(2425..2748)
                     /locus_tag="VF276_12505"
                     /pseudo
     CDS             complement(2425..2748)
                     /locus_tag="VF276_12505"
                     /inference="COORDINATES: protein motif:HMM:NF014560.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
BASE COUNT          444 a          945 c          915 g          569 t
ORIGIN      
        1 gcgccattgc ctggagcgcc gtctacaatc cgcattggga taacgacccg gatgcctatg
       61 ttgccgccgg ggtctgcccc tggggccgca cccgccgcct cggtaagttg ctgctgcccg
      121 acgcccggtt taaagcctat ctgggcgttc aacacgtcga tccccgcctg agcgggtggc
      181 cgggcctcga cgcaccccaa gcgcgcttct tcgtctcgtg gttcgccggc ggccagtgtc
      241 tgggcttgcg ggtcgcgccg ggcatggccg ccgcccgcga cctcctctgg gccgcctgga
      301 cccagcagca gccccttggc gcggcggggg gtgcccggca atgagacgct cagccccggc
      361 cccgccctgg cacccgatca cctccgggtg gtccacggtg tttctgctgc ccctggccgg
      421 cggcctcgtc accgccgcca acctgctgcg cctgggccgc gccgacaagg ccgtctttgc
      481 cgtgctgacc ggcctcgcct ccaccgcgat cgtgctggcg ctctggagcc agggtcccac
      541 ctgggctaac cgcaccttcc tgaccctgct gattttgagc ttcggcgacg ccgggttgtt
      601 cctgtatttg cagggcgccg actaccgggc ctgggccgac gaccacccgg atcgcggccc
      661 gacgagcgcg tgggttgcgg ccatctgggc gctgctcggc ttcatgctga tgatcgtgat
      721 ctgggtggcc tgggcggtgc tgctcgacct cggcggcccc cggccccgct gattcggctg
      781 ggccgcgggc cggttggaaa ccggccccta caacgcctgc gcgggcgcgg gccggttgga
      841 aaccggcccc tacagcgaac aaaatcgccc cgaccggctg ccgatctctt ataccttggt
      901 gtgggccgtc accgcgacct cgctgccgtc ctctgccgtg acgatctcgt agtcgccgaa
      961 caggttgggc gcctcctgct gcaacacccg caagatcttg atcgccagcc ggcggatctc
     1021 cggctcggag taggggctgg cgcgcatctc aataaagtgg cgccaggagc gggcgttggc
     1081 cgtgactacg atcttggtct cggtggcgtt gggcagcacg gcccgcgcgg cctggcgggc
     1141 catcttgcgg cgcaacgtct tgctcggcac attaacgaac tgcttgtcga gcaactccac
     1201 cagcgccaca taggcttcct gcgccgcggc gatggcctgc tcaaaaagcg cgtgcgcctc
     1261 cgggttggcg gcaatggcct ccggttccac gaagtccgcc gtgctctcgt ccacgtaccg
     1321 ttggctcaac tggctgttat gagtcacgat cccgttggcg acaaagttgt ggtgcggccc
     1381 ggccatctca atatcgtagg tatcctgttc ccccgcgtag gtgacgcttt tgatgcgcag
     1441 gaacttgtac gttacgctac gacgaccacg gggttgagga gcagcgcgga gctctgtgac
     1501 ttgcagggcc ggcacgccga atcgttccgc gtattcggcc tcgtggttgt tgaccttgcg
     1561 gtggcaggtg gcacacaagc tcaccagatt atccagatcc gcacgcagat ccggccgata
     1621 ccagatcggc aagatgtgat ggacggtcaa ccgaccccca actctctcgc acagccggca
     1681 gcggtagctg tcgcgctcaa ggaccagcgg gcgaagatcc ttcatgggtc gccgcaaggt
     1741 gatcgcttcc cgcgtgatac cgctacgcca ttgcggattc ttgtcccctc gcttttgctc
     1801 tgacagccgc acccgcgtct cagcggtgtg atgccaacct gcctggtatt gtttgccctt
     1861 gttccacggt tccacacctt tagtccacga ccccagtggc ttttgcaggt tcagcttgcg
     1921 gacccaggcg cgaatggtat gtggggagac accggccagt tcggcaatcg ttgcctggtc
     1981 cagactttgg actacataat gatcgtgcag ccagttataa tcctggtacg ccggttgtcc
     2041 gttcgttgca atcggcgtgt ccagggtatc cgccagcgcg atattgccgg gcgataaggt
     2101 taagctgccg accacttgat gcaggggctg ccagcccgac ggcgtcagga agcggtggtc
     2161 gcgcgagcag gtgatcgcct tgccatcctc caactcgacc cgaaacaccg gcttgactcc
     2221 cgaatagacc accgagcgga cagcgccggt tgtgaaggta ttcgtcgttt cgtcgagaca
     2281 acgcagccgt agcaggggga tgcgggaccg tccgtgcggg gtctgggtca tttcatataa
     2341 ttcgcgcagg gttcgcttcg taggcccgct gcgccggccc gatgccatat gctcactata
     2401 gatgagcgtg tctcctgtca agcagtagct gaatcccgcc ctgtggcgaa tcaactcatg
     2461 ggtcaggcta cgcgacacgc cggtgaagag gaaggtccag gtggcgtgct cgatgacgct
     2521 gccgtgcttg gcttccagga tgttgtcgat gtactcgtgg ttggtcttgc ggcccttccc
     2581 gaacgacatg tagcagaccc ggccgcccac ctcgaccacc ttctcggcgg cggaggtcgc
     2641 atcggtcgcc caggtcatac cctcgtcggt gataaactgg tcgatggcgg cctcgtcaag
     2701 ctgctggcga gcgatcagga agacttgcgg ttcacgcagg atacgcatgg gcagatcctt
     2761 tcggctgaag ggcagccgcc gcgagccatg tcgcggggcg cggtcagaaa gttcagcgca
     2821 ttatagcaca aggcctcgga gggtggtatc tgcttgcggc cggcgacctc aac
//
LOCUS       DASUYC010000760         2115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_903369,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000760 DASUYC010000000
VERSION     DASUYC010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903369"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="VF276_12510"
     CDS             complement(<1..647)
                     /locus_tag="VF276_12510"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6607727.1"
                     /translation="MGFLSRFAAPKRVGEIFEPSVADLIASGTRLAAAGQAAEALALF
                     DRALAREPLSVPGWYNRALVLGRTGRWDEALAAYDRVLVLDPDYAQAWYNKGVLLDAQ
                     GELPEGLAAYNRALGLDPTWADAWFSKGVLLAGMERPGEAVPCLEHARRLGHPLAAGY
                     LQQMQRRAVNAADAAIVAHPLDLAAWCNKGRVLADFGRHEEALFAYERALAIRPAS"
     gene            complement(780..1604)
                     /locus_tag="VF276_12515"
     CDS             complement(780..1604)
                     /locus_tag="VF276_12515"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6607728.1"
                     /translation="MRVTSLASGSSGNAFLVEAGTTTLLLDAGLPAPALERYLWLRNI
                     APRTLSAIFVSHEHIDHVRGAGMLSRRHGIPVVANAATFIAGVDALGTAAEQVMLPVG
                     GECRVGPVTVRTFSVSHDAADTVGFWVEAEGRHVCICTDLGIDTPSIADPLAAADLLV
                     LEANHDLDRLWRGPYPPALKRRVAGPRGHLANADAARLVRDLAEDGRPRTIWLAHLSA
                     TNNTPALAYAAVADPLRCAEMTHCTVAVAARDRPSLVWDSAAPVDEARPIAAVSTR"
BASE COUNT          316 a          755 c          755 g          289 t
ORIGIN      
        1 gacgccgggc ggatggccag cgcccgctca taggcgaaga gggcttcttc gtgccgcccg
       61 aagtcggcca gcacgcggcc tttgttgcac cacgccgcca ggtcgagcgg gtgcgcgaca
      121 atggcggcat cggcggcgtt caccgcgcgc cgttgcatct gctggaggta gccggcggcc
      181 agcggatggc ccaaccggcg ggcgtgctcc aggcagggca cggcctcgcc gggtcgctcc
      241 atccccgcca gcagcacgcc cttgctgaac caggcgtcgg cccaggtggg gtcgaggccg
      301 agggcgcgat tatatgccgc cagcccttcc ggcaactcgc cctgcgcgtc cagcaagacg
      361 cccttgttgt accaggcctg ggcgtagtcg ggatcgagca ccagcacgcg gtcataggcg
      421 gccagggcct cgtcccagcg cccggtgcga cccagcacca gcgcgcggtt gtaccagccg
      481 ggcaccgaca gcggttcgcg ggccagcgca cggtcgaaca aggccagcgc ctcggccgcc
      541 tggccggcgg cggccaggcg cgtgccgctc gcgatcaggt cggcgacgct cggctcgaag
      601 atctcgccga cccgcttcgg cgcggcgaac cggctcagga agcccacggg cgatctcccc
      661 cttcaactcc agaccatcct tcaacccgag gccgggccaa cgtgcggggc ggaggagtgc
      721 ggggccgggt cgatctcggc cctattatac tacggctgcg cttccttgtg gggagatcgc
      781 tagcgggtgg aaaccgccgc gatgggccgc gcctcatcga ctggcgcggc gctgtcccag
      841 accaggctgg ggcggtcgcg agcggccacg gcgaccgtgc agtgggtcat ctccgcacag
      901 cgcagcgggt cggcgacggc ggcgtaggcc agggccgggg tgttgttggt ggccgagagg
      961 tgggccagcc agatcgtgcg cgggcggcca tcctcggcga gatcgcgcac cagtcgggcg
     1021 gcgtcggcgt tcgccaggtg gccgcgcggc ccggccacgc gccgcttgag cgcgggcgga
     1081 taggggccgc gccacagccg gtcgaggtca tggttcgctt ccagcaccag caggtcggcg
     1141 gcggcgaggg gatcggcgat gctcggcgtg tcgatgccca ggtcggtgca gatgcagacg
     1201 tggcggccct cggcctcgac ccagaacccg accgtatcgg cggcgtcatg cgagacggag
     1261 aacgtccgca ccgtgaccgg cccgacccgg cactcgccgc ccaccggcag catcacctgc
     1321 tcggcggcgg tgcccagcgc gtccacgccc gcgatgaacg tggcggcgtt ggcgaccacc
     1381 gggatgccgt ggcggcgcga cagcatcccc gcgccgcgca catggtcgat gtgctcatgg
     1441 ctgacgaaga tggccgacag cgtgcgcggc gcgatgttgc gcagccacag gtagcgctcc
     1501 agcgcgggcg ccggcagccc ggcatccagc agcagggtcg tcgtcccggc ctccaccaga
     1561 aacgcattgc ccgagcttcc actcgccagc gacgttacgc gcacccgttt gtcctctcta
     1621 gttcccagaa cacgctattt gccgtccggc gcgggccggt ttgaaaccgg cccctacagc
     1681 gcggttacag gcgcgggccg gtttgaaacc ggcccctaca gcgcgattgt aggccctggg
     1741 ctggtattaa acccgctcct acaagcagac agatccctga accggaaacc ggcgtctcag
     1801 attgcggtgt aggggccggt ttgaaaccgg cccgtgccgc cgtcaccgcc ccgcaacccg
     1861 gcgtctcaga ttggccggtt tcaaaccggc ccgtgccgtc gcaccggaaa ctggcgtctc
     1921 taattgcggt gtaggggccg gtttgaaacc ggcccgcgcc gccgtcaccg cccccgccgg
     1981 ctatcggcat aatcgcgcag gcgggagggc agatcggcca gggaatcgag gcggatgtcc
     2041 gcctgggcgg cctccgggcg cagggcatgc aggtagcccc acggcccgcg ccgcagaaac
     2101 acggccagca tcccc
//
LOCUS       DASUYC010000761         3041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_398756,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000761 DASUYC010000000
VERSION     DASUYC010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398756"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="VF276_12520"
     CDS             complement(<1..954)
                     /locus_tag="VF276_12520"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEX6607729.1"
                     /translation="MFDARLHRGQSSRLLVLALLAVLLTGSLTPAAAHPAPTGPAPAM
                     PGGPQEPIGPCTLSFTDLPSSSPFYSFVQTLVCRSIVGGYSDNTFRPGANVTRGQLAK
                     FVSNAAGYQDAIPATQQTFRDVPPDNPFWMFIERVHAHNVVGGYADGSFQPGNNVTRG
                     QIAKFVSNAAVLTDSIPATQQTFADVPAANPFWLYIERAAQAGVVGGYSDGTFRPTNN
                     VTRGQTAKFIANGFFPASAQQLIAAAYQAGQIDYPTSLLYRAYALFGDTRLPASYQGV
                     GSTGDAMLFREIAAGRATLPASVLAQLQPFLVRPDDPASILN"
     gene            1332..2753
                     /gene="aspA"
                     /locus_tag="VF276_12525"
     CDS             1332..2753
                     /gene="aspA"
                     /locus_tag="VF276_12525"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEX6607730.1"
                     /translation="MDDASQRYEHDLLGERAVPAEAYYGVHTLRAVENFPITGTPVAR
                     YPALIHALAAVKQAAAQANKDLGLLDPVKADAIITACEEIRAGRLLDQFVVDVIQGGA
                     GTSTNMNANEVIANRALELLGHPRGDYAVVHPLEHVNMSQSTNDVYPTAVKLALHVLI
                     YALVDAMAEVRAAFEGKAEKFAGVLKMGRTQLQDAVPMTLGQEFSTYAIMLGEDEARL
                     REASQLLQEINLGATAIGTGINAHPEYAARVCRALAAITGVPLITAPNLVEATQDAGA
                     FVQLSGVLKRVAVKLSKICNDLRLLASGPRAGLGEINLPAMQAGSSIMPGKVNPVIPE
                     VVNQIAFEVIGNDITVSFAAEAGQLQLNAFEPIIAYSLFQSLTHLRAGCLTLGERCVR
                     GITANPAHLRRMVEHSIGMVTALTPYIGYERASAIAQEALATGQSVYNLTLARGFLTQ
                     AQLDDILRPEVLTRPRPTLPARE"
BASE COUNT          542 a         1002 c         1042 g          455 t
ORIGIN      
        1 attgaggatg ctggccgggt cgtcgggacg caccaggaac ggctgcaact gcgccagcac
       61 gctcgccggc aacgtcgccc ggccggctgc gatctcgcgg aacaacatcg cgtcgccggt
      121 cgaacccaca ccctgatagg acgcgggcag ccgggtgtcg ccgaagagcg cgtaggctcg
      181 ataaagcagc gaggtcgggt agtcgatctg cccggcttga tacgccgccg cgatcaactg
      241 ctgcgcgctc gcgggaaaga acccgttggc gataaacttg gcggtttgcc cgcgggtcac
      301 gttgttggtg gggcggaaag tgccgtcgct gtagccgcca accacaccgg cctgcgccgc
      361 ccgttcgata tataaccaga aggggttggc cgccggcacg tcggcgaagg tttgctgcgt
      421 ggccgggatg gagtcggtca acaccgccgc gttgctgacg aacttggcga tctggccgcg
      481 cgtgacgttg ttgcccggct gaaagctgcc gtcggcgtag ccgccgacca cgttatgggc
      541 atgcacccgc tcgatgaaca tccagaacgg gttgtccggc ggcacatcgc ggaaggtctg
      601 ctgcgtggcc gggatggcgt cctggtagcc ggcagcgttg ctgacgaact tggcaagctg
      661 gccgcgcgtc acgttggcgc cgggccggaa ggtattgtcg ctgtagccgc cgacgatgct
      721 gcggcaaacc agcgtttgca caaagctgta aaaggggctg ctcgaaggca gatcggtgaa
      781 actgagggtg caggggccaa ttggttcctg cgggccgccg ggcatagcgg gtgccgggcc
      841 ggtgggcgcc gggtgtgccg ccgccggggt gaggctgccg gtcagcagca cggccagcag
      901 ggccaggacc agcaggcgcg aggattgtcc ccggtgtaga cgagcgtcaa acaacgtgag
      961 cctcccgtga agaactacta gagcggcccg gctacgcgcg cccccacgga ccgcgggaaa
     1021 gcagataacg taggtgtcaa tggccgggca ccgggacaga cggggtaagt gcgagcgccg
     1081 gttaagcaga ttatacgggc gccctgtgcg atctgatccg gccaaaaagc cgacaaaaat
     1141 ctaaccggca gcccgacata gcgataggcg gagatacatg ttgcggaaag gaagaggaca
     1201 cggcatacta ggcgtataac cggcgcgccc ggcgggatgc ggggccatgc cccggcaccc
     1261 tggcgcagcg gatcggggct gatcgctggc gcggctacac tgccgtcaca tccaccgtgg
     1321 gaggatatgt catggacgat gcttcgcagc gctatgaaca tgatctgctg ggcgagcgcg
     1381 ccgtgcccgc cgaggcctac tacggcgtgc ataccctgcg cgccgtggag aacttcccca
     1441 tcaccggcac gcccgtcgcc cgctaccccg cgctgatcca cgcgctggcc gccgtcaaac
     1501 aggccgctgc ccaggccaac aaggacctgg gcctgctcga ccccgtcaag gccgacgcga
     1561 tcatcaccgc ctgcgaggag atccgcgcgg gtcgcctcct cgatcaattc gtcgtcgacg
     1621 tgatccaggg cggggcgggc acctcgacca atatgaacgc caacgaagtc atcgccaacc
     1681 gggcgctgga actgctgggc cacccgcgcg gcgactacgc ggtcgttcac ccgctggaac
     1741 acgtcaatat gagccagagc accaacgacg tgtaccccac cgcggtgaaa ctggcgctgc
     1801 atgtgctgat ctacgccctg gtcgacgcca tggccgaagt gcgcgcggcg ttcgagggca
     1861 aggccgagaa gtttgccggg gtgctgaaga tggggcgcac ccagttgcag gacgccgtgc
     1921 ccatgacgct gggccaggag ttcagcacct acgccatcat gctgggcgag gacgaggccc
     1981 gcttgcgcga ggcgtcacaa ctgctgcagg agatcaacct gggcgccacg gctatcggca
     2041 ccggcatcaa cgcgcacccg gaatacgccg cccgcgtgtg ccgcgcccta gccgccatca
     2101 ccggcgtccc gctgataacg gcgcccaacc tggtcgaggc cacgcaggac gccggggcct
     2161 ttgtgcagct ttcgggcgtg ttgaagcgcg tggcggtcaa gctgtcgaag atctgcaacg
     2221 acctgcggct gctggcgtcg gggccacggg cggggctggg cgagatcaac ctgcccgcca
     2281 tgcaggccgg gtccagcatc atgccgggca aggtcaaccc ggtgatcccg gaggtagtga
     2341 accagatcgc cttcgaggtc atcggcaacg atatcacggt gagctttgcc gccgaggccg
     2401 gccagctcca gctcaatgcc ttcgagccga tcatcgccta cagcctgttc cagagcctga
     2461 cccacctgcg cgcgggctgc ctgactctgg gcgagcgctg cgtgcgcggc attaccgcca
     2521 accccgcgca cctgcggcgc atggtcgaac actctatcgg catggtgacg gcgctcaccc
     2581 cgtacattgg ctatgagcgg gcgtcggcca tcgcccagga ggccctggcg accggccaga
     2641 gcgtctataa cctgacgctg gcccgcggtt tcctgaccca ggcccaactt gacgacatcc
     2701 tccgccccga ggtgctgacc cgcccgcgcc ccacgctgcc cgcgcgggaa tagccgtcgc
     2761 ccggcccggt tgcccggccc ctgaacgggc gggcaacatt tacgaagccc gcctgcgcgg
     2821 gcgggattca tccgggaaat aggctgcgca ggcggccttg gtaagggtcg cccgcccgtt
     2881 caggggtcgg gcgacccctc tcacggaccg ggcaccgccc cctcgcgcag ggctgtggcc
     2941 cgctcatgcc aggcgatcag gcgatagagg gtcaggaaca gcaggctgat gccggtggcg
     3001 gcgtagtaca gccagaccca gagattggtg ctgtcggtgc c
//
LOCUS       DASUYC010000762         2498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_812314,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000762 DASUYC010000000
VERSION     DASUYC010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812314"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="VF276_12530"
     CDS             complement(<1..778)
                     /locus_tag="VF276_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013157291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6607731.1"
                     /translation="MTDLVIETTGLRKIYGRKAAVSDLTLQVPRGEVFGFLGPNGAGK
                     STAVKMLLGLVQPSGGQARVLGQPPGHVATMARVGFLPEHFRFHEWLQAAEFLDLHGK
                     LYGMDAAARKRRIPELLDLVGLTEQAHRTLARFSKGMLQRIGLAQALLNDPALVFLDE
                     PTSALDPFGRLLVRNIIHDLKARGTTVFLNSHLLGEVEATCDRVSFIKEGRVLRTLAL
                     HDLEAGRLHVELRVDAVTPALLGALDAMSLDHHVVDGGHPD"
     gene            1066..1269
                     /gene="rpmI"
                     /locus_tag="VF276_12535"
     CDS             1066..1269
                     /gene="rpmI"
                     /locus_tag="VF276_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX6607732.1"
                     /translation="MPKMKTHKGSARRFKITGTGIIMRTKGMKSHFRRRKSSRVKQQF
                     DRMTPLDPTQVPRVKRLLPYGLD"
     gene            1369..1710
                     /gene="rplT"
                     /locus_tag="VF276_12540"
                     /pseudo
     CDS             1369..1710
                     /gene="rplT"
                     /locus_tag="VF276_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201247.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
     gene            2026..>2498
                     /locus_tag="VF276_12545"
     CDS             2026..>2498
                     /locus_tag="VF276_12545"
                     /inference="COORDINATES: protein motif:HMM:NF019644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEX6607733.1"
                     /translation="MISSASNPTIKRVRGLGAPAPQHRRAERAFVVEGVRAVEEALNE
                     GARPGLVLYDAEALRQTPRGTQLLGRIARLPEAQETSRGALAAATETVQPQGVLAVFP
                     FVDWPAAPPEAAPLYLILDGIRDPGNLGTILRGAEAAGVTACWLTPDCVDLYNPKV"
BASE COUNT          442 a          833 c          844 g          379 t
ORIGIN      
        1 ggtcgggatg cccgccgtcc acgacgtgat gatccaggct catggcgtcg agcgcaccga
       61 gcagcgcggg cgtcacggcg tccacgcgca actcgacatg caggcgcccg gcctccaggt
      121 cgtgcagggc cagggtgcgc agcacgcggc cctccttgat gaacgacacc cggtcgcagg
      181 tggcctccac ctcgcccagc aggtgcgagt tgaggaagac ggtcgtgccg cgcgccttca
      241 ggtcatggat gatgttgcgc accagcagcc gcccgaacgg gtcgagcgcc gaggtcggtt
      301 cgtcgaggaa gacgagcgcg gggtcgttca gcagcgcctg ggccagcccg atgcgctgca
      361 acatgccctt ggagaagcgg gccagggtgc ggtgcgcctg ctccgtcagc ccgacgagat
      421 cgagcaattc ggggatgcgg cgcttgcggg cggcggcgtc catgccgtaa agcttgccgt
      481 gcaggtcgag aaactcggcg gcctggagcc attcgtggaa gcgaaagtgc tccggcaaaa
      541 agccgacgcg ggccatggtg gcgacgtggc cgggcggctg ccccagcacg cgcgcctgcc
      601 cgccgctggg ctgcaccagc cccagcagca tcttgaccgc cgtgctcttg cccgcgccgt
      661 tcgggccgag gaagccgaag acttcgccgc gcggcacctg caaggtcagg tcggacacgg
      721 cagccttgcg gccatagatc ttgcgcaggc cggtggtctc gatcaccaga tcggtcatac
      781 atactcccct attcgtccgg attgcgcgcg ccggggcgtg cgctttgttc tataaaccgc
      841 gccgccgcct cgcatattac acgctacgcc gcaggctgtc cagaagttgc aggttcgcgc
      901 cggatcgggt ataatcactt cttgtgaccc gcgctaaggg atcacatata ttaccgttaa
      961 tgggctgccg tggagttatc aacctggtcg ttcgcgaccg tcccggcccg ccccagctag
     1021 atcgcatagc aggcgctatg cgcgctcgaa aggagcacgg tccccatgcc gaaaatgaag
     1081 acccacaagg ggtcggcgcg ccggtttaag atcaccggca cgggcatcat catgcgcacg
     1141 aagggtatga agagccactt ccgccggcgc aagtcgagcc gcgtgaagca gcaattcgac
     1201 cgcatgacac ccctggaccc cacgcaagtg ccccgcgtca agcggttgct gccttacggt
     1261 ttggattaag ctttcagccg tcagccgtca gccgtcagga gatgtaacga tatgccgggc
     1321 tgcgggttgg tacgtgaaag cgttttttgt taaggagaag gaactgacat gcctcgcgta
     1381 aaacgtgggt ttactcgcca ccggcggcac aataaagttc tggggatggt caagggccac
     1441 cagacaagcc gcggtcattt gttcaggaca gcgaatcagg aaatgatgcg gtcgctgatg
     1501 tacgcctatc gggaccgccg cacccgcaag cgcgatatgc gcgcgctctg gatcgtgcgc
     1561 atcaacgccg cggcccgcga gaacgggctg ccctacaacc gcttcattgc cggcatcaat
     1621 ggcctgggcc tggagctgga ccgcaagatg ctggccgaga tggccgtgaa cgatccgcat
     1681 ggcttcgccg tgctggccgc acgcatcaag ggcgagcccg agccggtgcg taaagccgcc
     1741 ccggcccccg ctgccgccgc gcccgccacc gaggccaccg caagcgccac ggcgccggag
     1801 cccgccgccg agaccaccgt cgtcgctccg gtggcgcagg gcgaggtgga gaccgaagag
     1861 gaaaaggacc tggaagccgc gggcgtcccc gaggagcagc cggcggagca ggacgcgcgg
     1921 cctgagcacg gcgatcccca gcagggcgag tcgcgctaac gccgatgtga tccccgatgt
     1981 ggcgagggtt agtccctcgc cacatttttg tttgtggtgt gagatatgat tagcagcgcg
     2041 agcaacccca caatcaagcg ggtgcgtggc ctgggcgcac cggccccgca gcaccgccgc
     2101 gccgagcgcg ccttcgtcgt cgagggcgtg cgggcggtcg aggaggcgct gaacgaaggg
     2161 gcccggcccg gcctggtgct gtacgacgcc gaggcgctgc gccagacgcc acgcggcacg
     2221 cagttgctgg ggcgcatcgc ccggctgccg gaagcgcagg aaaccagccg gggcgcgctg
     2281 gcggcagcaa ccgagacggt gcagccgcag ggcgtgctgg ctgtcttccc tttcgtggac
     2341 tggcccgccg ccccgcccga agccgccccg ctctacctca tcctcgacgg catccgcgac
     2401 cccggcaacc tgggcacgat cctgcgcggg gccgaggcag cgggcgtcac cgcatgctgg
     2461 ctcacacccg attgcgtcga tctctacaac cccaaggt
//
LOCUS       DASUYC010000763         2764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1272295, whole genome shotgun sequence.
ACCESSION   DASUYC010000763 DASUYC010000000
VERSION     DASUYC010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1272295"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="VF276_12550"
     CDS             complement(<1..564)
                     /locus_tag="VF276_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607734.1"
                     /translation="MRRLFSPARVALLAGLSLVLAACGAAVTPTVPPPTPVPTVPPRA
                     DPELLKTALANMSALRSFTWDLTQEYTVGLGGYTYPRRRDLLDRVWGDADAAHQTYRF
                     AVRHVSGFTQTAVARDDKVYAINPDGSATELHEVWLFLQRSVFQQSMWQRPTESFDYT
                     AMLTKGVTVTLGTPPSEVIDGAPTTHLV"
     gene            complement(609..2369)
                     /locus_tag="VF276_12555"
     CDS             complement(609..2369)
                     /locus_tag="VF276_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX6607735.1"
                     /translation="MRPVFARARLAGLVGLSLVLAACGAAATPTGPPATPTVPPPTPV
                     PTVAPRPDPEFLHTALTNMPALQSFTWVLTQDITYSLPDNSYKPLMNELFDRVQGVAD
                     VTNRTHQFEIAIEARGQTGSATIVVRDDERYFIQPDGSAQELPGESAIGYYWFFFQQG
                     MWQPAPGFNYEPALSRGVPVTLSTPPSEVIDGVPTTHLAMDLRAMPEVFWSTDALSGT
                     VEFWVDPQPPARVRRLALTAAVPTGDVPGPGILSPTEPVAGFLVGGDRLQLRRLPDGG
                     LLRTVQLAPPARPSDIEILGFTADGRSVVGVALPHFYRWSTQDGHIESESPEVNGFNP
                     GYRLSPDGGWLALGDNGIGLYRTSDWQRVATLESVLPGTHALTFSPDGRLVAALAPPA
                     GGERYHSPRRVLQIWRTDGTLVRTQTLKDQAEAEIDALLFTADSRQLVLHTRADGAVE
                     IRQVADGALLHTLAPAVPALAALAVSPDGRRLALASDTAGVYVWDLAHPTAEAVRLEP
                     GVAKAAPGGLPHFSADGSRLLFSGKGVRVWAVADLAAPPVSWPLQEMKTYRYVWQWGH
                     FNEPVSIPPLRLAPTATPEP"
BASE COUNT          425 a          909 c         1031 g          399 t
ORIGIN      
        1 aactagatgg gtcgtgggcg cgccgtcaat cacttcgctg gggggcgtgc ccagcgtcac
       61 cgtgactccc ttggtcagca tcgccgtgta gtcgaacgac tcggtgggcc gctgccacat
      121 gctctgttgg aagacggacc gctggagaaa gagccacact tcatgtaatt ctgtcgcgct
      181 gccgtcggga ttgatcgcgt agactttgtc atcgcgggcg acggcggtct gcgtgaaccc
      241 gctcacatga cggacggcga accggtaggt ctggtgggcc gcatcggcgt cgccccagac
      301 gcggtccaag agatcgcgtc tgcgcgggta ggtgtaaccg cccaggccca cggtgtactc
      361 ctgggtcagg tcccaggtaa agctccggag ggcagacata ttggcgagcg ccgttttcag
      421 cagttcaggg tcggcgcgcg gcgggacggt gggcacgggg gtgggcggcg gcacggtggg
      481 ggtgacggcg gcgccgcagg ccgccagcac caggctcagc ccggccagca gggccacgcg
      541 ggccggggag aacaggcgac gcatacgaga ttcctccttt tgcacgggag cggcggtgcg
      601 cccgcggctc agggctccgg cgtggccgtg ggcgccaggc gcagcggcgg aatgctcacc
      661 ggctcgttga agtggcccca ctgccagaca tagcgatagg tcttcatctc ttgcagcggc
      721 caggacacgg gcggcgcggc cagatcggcc accgcccaca cccgcacgcc cttcccgctg
      781 aacagcagcc ggctgccgtc cgcgctgaag tgcggcaggc cgcccggcgc ggctttcgcc
      841 acccccggct ccaggcgcac cgcctccgcg gtcgggtgcg ccaggtccca gacataaacc
      901 ccggccgtgt ccgaggcgag cgccagccgg cgaccgtccg ggctgacggc cagggccgcc
      961 agagccggca ccgcgggcgc cagcgtgtgc agcagtgcgc cgtcggccac ctggcggatc
     1021 tccaccgcgc cgtccgcgcg cgtgtgcagc accaattgcc ggctatccgc ggtgaacagc
     1081 agagcgtcaa tttcggcttc ggcctgatcc ttcagggttt gcgtgcgcac cagggtgccg
     1141 tcggtgcgcc agatctgcaa gacccggcgc ggcgagtggt agcgttctcc gcccgccggc
     1201 ggggccaggg cagccaccag acgcccgtcg ggcgagaagg tcagagcgtg ggtgccgggc
     1261 aggaccgatt ccagcgtggc gacgcgctgc cagtcgctgg tgcgatacag gccgatcccg
     1321 ttgtcgccca acgccagcca cccgccgtcg gggctgagcc tgtagcccgg attgaagccg
     1381 ttgacctcgg gcgattcgct ttcgatgtgc ccgtcctggg tgctccagcg gtagaagtgg
     1441 gggagcgcca ccccgaccac gctccggccg tcggccgtga agcccaagat ttcgatatca
     1501 ctgggccggg cgggtggggc cagttggacg gtgcgcagca agccgccatc gggcaggcgg
     1561 cggagctgca ggcgatcgcc gcccaccagg aagccggcga ccggctcggt ggggctgagg
     1621 atgcccggcc ccggcacgtc gccggttggg acggcagccg tcaaggccag gcggcgcacg
     1681 cgggcggggg gctgcgggtc cacccagaac tccaccgtgc cgctcaatgc atccgtgctc
     1741 cagaatacct ccggcatggc gcggagatcc atggccagat gcgtagtcgg cacgccgtcg
     1801 atcacttcgc tgggcggagt actcagcgtc acgggcactc ctctgctcag cgccggctca
     1861 taattgaaac caggggcggg ctgccacatg ccctgctgga agaagaacca atagtaaccg
     1921 atagccgatt cgccgggcaa ctcttgcgcg ctgccgtcgg gctggatgaa atacctctcg
     1981 tcgtcgcgca cgacgatcgt cgcagatccc gtctggcccc tcgcctcgat tgcgatctcg
     2041 aactggtgcg tccggttggt cacatcggcc acgccttgga cgcgatcgaa gagttcgttc
     2101 atcagcggct tatagctgtt gtccggcagc gaataggtga tgtcctgggt caggacccag
     2161 gtgaaactct ggagggcggg catattggtg agcgccgtat gcaggaattc gggatcgggg
     2221 cgcggggcca cggtgggcac cggggtgggc ggcggcacgg tgggggtggc cggcggcccg
     2281 gtaggggtgg ccgcggcccc gcacgcggcc agcaccaggc tcagcccgac gagcccggcc
     2341 aggcgggcgc gggcgaacac gggacgcata ggggggacct ccttgtgcgc cggagggcgc
     2401 accagagcga ggaccgccgg atccgctgcc gggggggccg ggccggcgcg gggatgcggt
     2461 tggtggcgcc gcgaccagac ccgcgcccgg cgccaaccag gggctccggg gggcggaagg
     2521 gaacggagtt gcggaacatg gcaacctccg cgacgatggg acccagggat ggcgggccgc
     2581 ccgcgcgccg taagcgcgat ccgcagacgg ccccaagcct agcataccgc gtcccggtga
     2641 cagccgcggt tacgccgggt agcgattcgg taacagttgg ggccggtcgc cggggtggcg
     2701 agccggctta ttgccgggcg attctgcacc gcagcgtgtg atatgtgccg gtgggacaca
     2761 gggg
//
LOCUS       DASUYC010000764         2167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_349641,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000764 DASUYC010000000
VERSION     DASUYC010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349641"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..1006
                     /locus_tag="VF276_12560"
     CDS             176..1006
                     /locus_tag="VF276_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004654195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6607736.1"
                     /translation="MGSLKGRTLFITGGSRGIGRAIALRAARDGANVVIAAKSAEPHP
                     KLPGTIFSVAEEIEAAGGRALPLQVDVRDEEQVRAAMAKAAEHFGGIDCLVNNAGAIN
                     LMGVEHTPAKKYDLIQGVNHRATFLCAQAALPWLKQSPAAHILSLSPPINMAPHWLKP
                     FAPYTVSKYGMTILTLGMAEEFRDYGISCNTLWPATYIATAAVEVNLGGEAAAAVSRR
                     PEIMADAAHAILATGRGELTGQSLLDEQALARIGVTDLRDYACDPANADRLQRDFFLD
                     "
     gene            1288..>2167
                     /locus_tag="VF276_12565"
     CDS             1288..>2167
                     /locus_tag="VF276_12565"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX6607737.1"
                     /translation="MSKQDVITPVAFARKFAEIAPRLPKMVQGLLLASNTDPTKNVGL
                     GWAIEKATRENPHGTAVLYKDVRLTYTQFNQWANRMAHYFQAKGLQKGDVVAVYVENR
                     PELLATVVALGKIGVVSAMINTSQTGKVLTHSVNLVKPKAAIVGEELVDNFSEVLADL
                     HLDHKNLYWFADHDTLKTPGRAPKGFHNLAEEIHGQPVYNPASTTQIYPHDGLFYIYT
                     SGTTGLPKAAIFSHGRWMKAYGVFGYTMQLGKTDVLYATLPFYHATAMVVCWGSALAG
                     YAGVAMRRRFSASEFWN"
BASE COUNT          398 a          790 c          635 g          344 t
ORIGIN      
        1 ccgagaaaca gcaagtgaag aaacgccagc aactgctgct ggtccaggac tgaccccgtc
       61 ggtcggttgt aggtcgggct tcagcccgac aacaggtcgc cccgttcggg ctgaagcccg
      121 acctacagcg ttaatgcgtc gggctgaagc ccgacctaca gagaaggaga agtaaatggg
      181 atcgctcaag ggccgtaccc tgttcatcac cggcggcagc cgcggcatcg gccgcgccat
      241 cgcgctgcgc gccgcccgcg acggcgccaa cgtcgtcatt gccgccaagt cggcggagcc
      301 gcaccccaag ctgcccggca ccatcttcag cgtggcggag gagatcgagg ccgccggcgg
      361 tcgtgccctg cccctgcagg tggacgtgcg tgacgaggag caggtgcgcg ccgcgatggc
      421 gaaggccgcg gagcacttcg gcggcatcga ctgcctggtg aacaacgccg gcgccatcaa
      481 cctgatgggc gtggagcaca ccccggccaa gaagtacgac ctgatccagg gtgtcaacca
      541 ccgcgccacc ttcctctgcg cccaggctgc gctgccctgg ctcaagcagt cgcccgccgc
      601 gcacatcctc agcctgtcgc cgcccatcaa catggcgccg cactggctca agcccttcgc
      661 accctacacg gtgtccaagt acggcatgac catccttacc ctgggcatgg ccgaggagtt
      721 ccgcgactac ggcatctcct gcaacacgct ctggcccgcc acctacatcg ccaccgcggc
      781 ggtggaagtg aacctgggcg gcgaggcggc cgccgccgtc agccgtcgac ccgagatcat
      841 ggcggacgcc gcccatgcca tcctggccac cggccgcggc gaactcaccg ggcagtcgct
      901 gctcgacgag caggcgctgg cgcgcatcgg cgtcaccgac ctccgcgact acgcctgcga
      961 ccccgccaat gcggaccggc tgcagcgcga cttcttcctg gactgaaggc acagcccccg
     1021 catcggcagg cgatatattg tccgacaata tatcgccgcc ggtcgggccg gaatggcctg
     1081 ctgaattggc ccgatttgcc gatttctcaa taacttgcag cacttttgcc gacatgcgcg
     1141 ctgcgtattt tccggcttga atccggacca tcggccgggt acctttgatc ctcgccttgg
     1201 agcggtcctt tccgctccgt ccagtcgact cgcccataag aattaccggc cagcggtcat
     1261 gcattccgct cccgaggaca ctccagcatg agcaagcaag acgtcattac ccccgtagcc
     1321 ttcgcccgca agttcgcgga aatcgccccc cgtctcccga agatggtgca ggggctgttg
     1381 ctcgcctcca acaccgaccc gaccaagaac gtcggcctcg gctgggcgat cgagaaggcc
     1441 acgcgtgaaa atccgcatgg aaccgcggtg ctgtacaagg acgtccgcct cacctacacc
     1501 cagttcaacc agtgggccaa ccgcatggcc cattacttcc aggccaaggg cctgcagaag
     1561 ggcgacgtcg tcgccgtcta tgtcgagaac cgccccgagc tgctcgccac cgtggtcgcg
     1621 ctcggcaaga ttggcgtggt ttccgccatg atcaacacct cccagaccgg caaggtcctg
     1681 acgcacagcg tcaacctggt gaagcccaag gccgccatcg tcggcgagga gctggtcgac
     1741 aacttcagcg aggtgctggc cgacctgcac ctggaccaca agaacctgta ctggttcgcc
     1801 gaccacgaca ccctgaagac ccccggccgc gcgcccaagg gcttccacaa cctggccgag
     1861 gaaatccacg gccagccggt gtacaacccg gcatcgacca cccagatcta cccgcacgac
     1921 ggcctcttct acatctacac ctccggcacc accggcctgc ccaaggccgc gatcttcagc
     1981 cacggccgct ggatgaaggc ctacggcgtg ttcggctata ccatgcagct gggcaagacc
     2041 gacgtgctct acgccaccct gcccttctat cacgccaccg ccatggtggt gtgctggggc
     2101 tccgcgctgg cgggttatgc cggcgtcgcc atgcgccgcc gcttctccgc ctcggagttc
     2161 tggaatg
//
LOCUS       DASUYC010000765         2938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1272710, whole genome shotgun sequence.
ACCESSION   DASUYC010000765 DASUYC010000000
VERSION     DASUYC010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1272710"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /gene="pheS"
                     /locus_tag="VF276_12570"
     CDS             <1..282
                     /gene="pheS"
                     /locus_tag="VF276_12570"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481793.1"
                     /note="catalyzes a two-step reaction, first charging a
                     phenylalanine molecule by linking its carboxyl group to
                     the alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; forms a heterotetramer of
                     alpha(2)beta(2); binds two magnesium ions per tetramer;
                     type 1 subfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX6607738.1"
                     /translation="DYFSYVEPGVDLSIDCLICGGTGCRSCKFTGFLEIMGAGMVHPQ
                     VLRNGGIDPDVYSGFAFGMGVERIANLRHAVDDIRLYYTNDLRLLRQFS"
     gene            295..2910
                     /gene="pheT"
                     /locus_tag="VF276_12575"
     CDS             295..2910
                     /gene="pheT"
                     /locus_tag="VF276_12575"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX6607739.1"
                     /translation="MATRVPLSWLREYIDIQVPPQELANRLILAGLEVERIDTIGSEW
                     DKVVVGRIATLDRHPNADRLWVTQVDYGGEAPQQVVTGAPNLHLGAMVPLAFPGAVLI
                     DGHSDAHEKITLKPGKLRGIESIGMVCSELELGISDEHEGIMLLPEDAPLGAPLQEVL
                     GDIVLEIAVTPNLGRILSLMGVAREIKALFGGEVHQPDAGWKPDGPPIDELLSVEIED
                     PDLCSRYSAAVIRNVQIGPSPDWMQRRLTLAGMRPISNVVDITNYVMLEMGQPLHAFD
                     YDKVHEHRIIVRRAREGEHLTTIDHEDRVLDSNTLVIADPQESVGLAGVMGGADSEIG
                     PETATILLESANFDPRNIARTARQLHLPSEAASRFERTVDQQLTVPALKRAAELMRRY
                     AGGTIAGGYIDHFPAPKPPVTIALTQHEVERLLGIPVPASEIAGMLRRLEFRVEMPAD
                     AANDPHATLTVHVPSYRNDVTIPADLVEEVARMIGYDRIPETLLSGTLPPQFVNYDYN
                     DRERVRDLLAACGLDEVITYSPISSAALARLGSPTPPAESAPPETEQAGPGKRRGAAP
                     KVWRYLAYDENRAIIRIANPLSSEQDIMRPALLPGLLGALRTNLRNTPRLYLFEIGNI
                     FWPATPEDARQREEGAAAGRHTALAPGEAMMPVEPRRIGALLTGPRHPRSRFARAEAA
                     EEQTDFYDAKGVVETLLAHLNISGARYEPVLAPWYHPGRVAAVVLGDTLLGVVGELHP
                     RVLDAFEIGGRRVYAFDLDLDALLAAIPQRVRYSSISRYPAVTQDIALVVAETVPASR
                     VEALIRETGGKLLTDVLLFDIYTGPPVPAGHRSLAYTLTFQALDRTLSEDEVTKLRNK
                     IANRLKYEAGAELRA"
BASE COUNT          501 a         1010 c         1006 g          421 t
ORIGIN      
        1 gactacttct cctatgtgga gccgggcgtg gacctgtcca tcgactgcct gatctgcggt
       61 gggacgggct gccggtcctg caagttcacc gggttcctgg agatcatggg cgcgggcatg
      121 gtccacccgc aggtgctgcg caatggcggg atcgacccgg atgtctacag cggctttgcc
      181 ttcggcatgg gcgtggagcg catcgccaac ctgcgccacg cggtagacga tattcgcctg
      241 tattacacga acgacctgcg cctgttgcgc cagttcagct agggagaagc aacgatggct
      301 acaagagtac cgctctcgtg gctgcgagag tacatcgata tccaggtgcc gccccaggaa
      361 ctggcgaacc gcctcatcct ggccggcctg gaagtggaac gcatcgacac catcggcagc
      421 gagtgggata aggtggtggt cgggcggatc gccacgctgg accgccaccc caacgccgac
      481 cggctctggg tgacgcaggt ggactacggc ggcgaggccc cgcagcaggt ggtcaccgga
      541 gcgccgaacc tgcatctggg cgccatggtg ccgctagcgt tccctggcgc cgtgctgatc
      601 gacgggcata gcgatgcgca cgaaaagatc acgctcaagc cgggcaagct gcgcggcatc
      661 gagtccatcg gcatggtctg ttcggagctg gagctaggca tctccgacga acacgagggc
      721 atcatgctct tgccggagga cgccccgctg ggcgcgcccc tacaagaggt gctgggcgac
      781 atcgtgctgg agatcgccgt gacgcccaac ctggggcgca tcctaagcct catgggcgtg
      841 gcccgcgaga tcaaggcgct gttcggcggc gaggttcacc agcccgacgc cggctggaag
      901 ccggacggcc cgcccatcga cgaactgctc agcgtggaga tcgaagaccc ggacctctgc
      961 tcgcgctaca gcgcggcggt gatccgcaac gtgcagatcg gcccgtcgcc cgactggatg
     1021 cagcggcggc tcacgctagc cgggatgcgc ccgatcagca acgtggtgga catcaccaac
     1081 tacgtgatgc tggaaatggg tcagccgctg cacgccttcg actacgacaa ggtccatgag
     1141 catcgcatca tcgtgcgccg ggcgcgcgag ggcgaacacc tcacgacgat cgaccacgag
     1201 gaccgcgtgc tcgactcgaa cacgctggtc atcgccgacc cgcaggagtc ggtgggtctg
     1261 gccggcgtca tgggcggcgc cgacagcgag atcgggccgg agacggccac catcctgctg
     1321 gagtcggcca acttcgaccc gcgcaacatc gcgcgcacgg cgcggcagtt gcatctaccg
     1381 tcggaggcgg ccagccgctt cgagcgcacg gtggaccagc aactgaccgt cccggccctg
     1441 aagcgcgcgg cggagttgat gcgccggtat gccggcggca ctatcgccgg gggctacatc
     1501 gaccacttcc cggcgccgaa gccgcccgtg accatcgccc tgacgcagca cgaggtcgag
     1561 cggctgctgg gcatcccggt gcccgcctcc gagatcgccg gtatgctgcg ccggctggag
     1621 ttcagggtcg agatgccggc agacgccgcc aacgacccgc acgccacgct gacagtgcat
     1681 gtgccgtcgt accgcaacga cgtgaccatc cccgccgacc tggtggagga agtggcgcgc
     1741 atgatcggct acgaccgcat cccggagacg ctgctgtcgg ggacgctgcc gccgcagttc
     1801 gtgaactacg actacaacga ccgcgagcgg gtgcgcgacc tgctggccgc ctgcggcctc
     1861 gacgaagtga tcacctactc gcccatcagc agcgcggcgc tggcccggct ggggtcgcct
     1921 accccgcccg ccgagagcgc cccgcccgaa accgagcagg ccgggccggg caaacggcgc
     1981 ggcgccgcgc cgaaagtctg gcgctacctg gcctacgacg agaaccgggc gatcatccgc
     2041 atcgccaacc cgctgagcag cgagcaggac atcatgcgcc cggccctgct gcccggcctg
     2101 ctgggggcgc tgcgcacaaa cctgcgtaac acgccgcgcc tctacctgtt cgagatcggc
     2161 aacatcttct ggccggcgac gccggaggat gcccggcagc gcgaggaagg cgcggcggcg
     2221 ggccggcaca cggcgctcgc ccccggcgag gcgatgatgc cggtcgagcc acgccgtatc
     2281 ggcgcgctgc tgacggggcc gcgacacccg cgctcgcgct tcgcccgcgc tgaggcggcg
     2341 gaggagcaga ccgacttcta tgacgctaag ggcgtggtgg aaacgctgct ggcgcacctg
     2401 aacatctcag gcgcgcgcta cgagccggtg ctggcaccct ggtaccatcc gggccgggta
     2461 gcagcagtag tgctcggcga cacactgctg ggcgtggtcg gcgaactgca cccgcgcgtg
     2521 ctcgatgcct tcgagattgg cgggcggcgc gtctacgcgt tcgacctgga cctcgatgcg
     2581 ctgctggcgg ccatcccgca acgggtgcgc tacagcagca tcagccgcta cccggcggtg
     2641 acgcaggaca tcgcactggt cgtggccgag acggtcccgg cgagccgggt cgaggcgctg
     2701 attcgcgaaa cgggcggcaa actgctgacc gacgtgctgc tgttcgacat ctataccggg
     2761 ccaccggtgc ccgcgggcca tcgcagcctc gcctacacgc tcacgttcca ggcgctggac
     2821 cgcaccttga gcgaagacga ggtgacgaag ctgcgcaaca agatcgccaa ccggctgaag
     2881 tacgaagccg gcgccgaatt gcgcgcctga tccttccccc gtctgtcgcg gcggggcc
//
LOCUS       DASUYC010000766         2192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1506561, whole genome shotgun sequence.
ACCESSION   DASUYC010000766 DASUYC010000000
VERSION     DASUYC010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1506561"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2184)
                     /locus_tag="VF276_12580"
     CDS             complement(<1..2184)
                     /locus_tag="VF276_12580"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6607740.1"
                     /translation="MRHTSHGTAFGTWLKQQRQARGLSRRELAERIGCAAVTLVKIED
                     GERKPSPQVAELLAEQFAIPLDERTAFADFARADLPPHRGRALPDTEAPAPWRVLYRR
                     PSNLPAPPTAFIGREQTVDQAGRLLRSPAVRLLTLTGPPGIGKTRTALQVAGALVADF
                     ADGVFFVPLAPIRDPTLLVPAVAAALDLRERNDQSLLRVVKEHLRPRQILIVLDNLEQ
                     VVAAAPQIAELLETGPQIKMLLTSRTLLRIYGENELPVPPLSLPDPAHLPPLNQFTRY
                     EAVHLFVERARAAEPGFRVSGGNVRAVAEICVRLDGLPLAIELAAARVRTLPVAEMHV
                     QLGHRLALLTEGPVDRSPRQQTLRGAIEWSYELLDDSTRRLFRYLAVFVGGGDWQAVE
                     RVVGAGTTGALAAQLDVLIAQNLLRYEGGAEAPRLGMLETIREYARTQLEAGGEAAAV
                     EERHAGYYLELAERADEWLKGPEQAPWLARLEQEHANLRAALDWSRQAPERAAFGLRL
                     AAALGQFWELRGYMSEGRARLQAALAVDDPTVPLAVRARAYAALGRMELYRGDFAAAR
                     GPYEQSLQLRRAVGPASALARALTSVGIVKRALGEYGAARALHEESLALWRAEGDQAG
                     IASALNSLGKLALLEGGFAAARAFAAESAAIRRAMGDRYALAMSLDTLGEIARSEGHY
                     DAAGALYTEGLALCQEQGDKPLTANALHNLGYVALHMGDAAAAAAR"
BASE COUNT          324 a          827 c          721 g          320 t
ORIGIN      
        1 tcgcgccgcc gccgccgccg catcccccat gtggagggcc acatagccga ggttatgcag
       61 ggcattggcc gtcaacggct tgtcgccctg ttcctggcag agcgccaggc cctctgtgta
      121 aagcgcgccg gccgcgtcat agtggccttc gctccgcgcg atctccccca gcgtgtccag
      181 cgacatcgcc agggcgtagc gatcgcccat cgcccgccgg atggccgcac tctccgcagc
      241 gaacgcgcgc gccgcagcaa aaccgccctc cagcagggcc agtttgccca ggctgttgag
      301 ggcgctggcg atcccggcct ggtccccctc cgcccgccaa agcgccaggc tctcttcatg
      361 cagggcgcgg gccgccccgt actcccccag cgcgcgcttc acgatcccca cgctcgtcag
      421 cgcgcgggcg agggcggaag cggggccgac ggcccgccgc aattgcaggc tctgctcata
      481 cggcccgcgc gccgctgcaa agtcgcctcg atagagttcc atccgcccga gcgcggcata
      541 agcgcgcgcg cgcaccgcca ggggcacggt cgggtcatcg acggccaggg cggcttgcaa
      601 ccgggcgcgc ccttcgctca tatagccccg cagttcccag aactgtccca gggccgccgc
      661 caggcgcagg ccgaaggcag cgcgctccgg ggcttgccgg ctccaatcca gggcggcgcg
      721 cagattggcg tgttcctgct ccagacgggc gagccagggg gcttgctccg gtcccttcaa
      781 ccattcatcg gcccgctcgg ccagctccag gtaatagccg gcgtgccgct cctccaccgc
      841 cgccgcttct ccaccggcct ccaactgtgt gcgggcgtac tcgcggatgg tttccagcat
      901 ccccaggcgg ggcgcctccg ccccaccctc gtaccggagc aggttttggg cgatcagtac
      961 gtcgagttgg gcggccaacg caccggtggt gcccgcgccc accacgcgct ccaccgcctg
     1021 ccagtcgccg ccgccgacaa aaaccgccag gtaccggaaa agccggcggg tggagtcgtc
     1081 cagtagctca tagctccact cgatggcgcc gcgcaacgtt tgctggcggg ggctgcgatc
     1141 cacgggcccc tccgtgagca gcgccaggcg atgtccaagc tgcacgtgca tctccgccac
     1201 cggcagcgtg cgcacccgcg ccgccgccag ttcaatcgcc agcggtaggc catcgagccg
     1261 cacgcagatc tcggccaccg cgcgcacatt ccccccgctg acccgaaagc ccggctcggc
     1321 ggccctggca cgctccacaa agaggtgcac cgcctcatag cgtgtgaact ggttcagcgg
     1381 cggcaggtgg gccggatcgg gaagcgacag ggggggcacc ggcaattcgt tctcgccgta
     1441 gatccgcaag agggtgcggc tggtcagcag cattttgatc tgcggcccgg tttccaacag
     1501 ctcggcaatc tgcggcgctg ccgccaccac ctgctccaga ttgtcgagca cgatcaggat
     1561 ctgccggggc cgcaggtgct ccttgacgac ccgcagcaac gactgatcgt tcctttcgcg
     1621 cagatcgagc gcggcggcca cggcggggac cagcagggtc gggtcgcgga tcggcgccag
     1681 ggggacgaag aacacgccgt cggcaaagtc cgccaccagc gccccggcca cctggagcgc
     1741 cgtgcgcgtc ttgccgatgc ccggcggccc ggtgagcgtc aacagacgca ccgccgggct
     1801 gcgcagcaag cggccggcct ggtccaccgt ctgttcgcgc ccgataaagg cggtcggcgg
     1861 ggccggcaga ttgctgggcc ggcggtacag cacgcgccag ggcgccggcg cctccgtatc
     1921 cggcagcgcc cgcccgcggt gcggcggcag gtccgcgcgg gcaaaatcgg cgaaggccgt
     1981 ccgctcatcc agggggatcg cgaattgctc ggccagcaac tcggcgacct gcggcgacgg
     2041 cttgcgctcg ccgtcctcga ttttcacaag cgtcacagct gcgcagccga tgcgctcggc
     2101 cagttccctc cggctcagcc cgcgtgcttg ccgctgctgc ttcagccagg ttccaaatgc
     2161 cgttccgtga ctggtgtgcc gcactacttt at
//
LOCUS       DASUYC010000767         5176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1736822, whole genome shotgun sequence.
ACCESSION   DASUYC010000767 DASUYC010000000
VERSION     DASUYC010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1736822"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            260..1342
                     /gene="ltaE"
                     /locus_tag="VF276_12585"
     CDS             260..1342
                     /gene="ltaE"
                     /locus_tag="VF276_12585"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HEX6607741.1"
                     /translation="MPDSLIDLRSDTVTHPSPAMREAMYGAEVGDDVFGDDPTVIRLE
                     ALAAATLGKEAALFVPSGTMGNLCAVLTHCGRGDELIVGMEAHIRHAEAGGASVLGGV
                     AMWGIPNAPDGTLPLDAVAAAIRPVDHHFPHTALICIENTQNRCGGVPIGRDYMAALG
                     EIAHSHGLPLHLDGARIFNAAVAFGISAADLAAPADTVMFCLSKGLAAPVGSMLCGPR
                     AFIERARFNRKLVGGGMRQAGIIAAAGIVALTEMVDRLAEDHANARRLAEGLAGLPGL
                     QVDLTRPLTNMVYANVGATGWNADEFTARAAEAGVLFAPTDPLVVRLVTHYGITAADI
                     DTTLSRLGTILQEQATNAGQLAGATA"
     gene            complement(1490..1945)
                     /locus_tag="VF276_12590"
     CDS             complement(1490..1945)
                     /locus_tag="VF276_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HEX6607742.1"
                     /translation="MATSSSAARPTAGDIPLPIVRLNRWVLLVGIVLGLILQQPLLTT
                     LLFLILLPAVVIGQRASLIFRVGKRLFARQLPTAEREDRRLMRFNNTIALVLLGLAQP
                     AFLLGAPLVGWALAGTVALAAGMALAGFCVGCFIYYQLKLQRYRLLGTR"
     gene            complement(1984..2943)
                     /locus_tag="VF276_12595"
     CDS             complement(1984..2943)
                     /locus_tag="VF276_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607743.1"
                     /translation="MNEPIGILYEHPEWFQPLFAALDRRGLAYERIPAQEHRFDPAAR
                     SAPYSLVVNRVSPSSYLRDHARAIFYAQQYLAHLEEIGVPVVNGLRAYRLELSKARQL
                     QLLARLGLPHPRSRVVNAVAEIAPAAAELDYPIIVKPNVGGSGALMRRFDTPGELAAA
                     VADRELDSILGLDYTAIVQEYHPPRHDSIVRVEALDDRLLYAIRIYNDTQDSFNLCPA
                     DICQLPPPGQRGVKKPLRVEAAEPPAWVIEAVLQIFRANSIDIGGVEYLESARDGGLY
                     LYDINALSNFVTDAPRIVGFDPFEDFVDYLARRAGLAVGAVAP"
     gene            complement(3043..3645)
                     /locus_tag="VF276_12600"
     CDS             complement(3043..3645)
                     /locus_tag="VF276_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011330631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX6607744.1"
                     /translation="MSQILDEVLAANNEYAANFGDKGQLPMPPGRHFAILTCMDARLD
                     PAKYAGLAEGDAHVIRNAGGRASDDAIRSLVISYKLLGTREWFVIHHTDCGMLTFTDE
                     VIRGLLAQSLKTASVDENGWHDSGEGPGSNEAEFISFLTIRDQAQSVLDDVRRIRAHA
                     LVPGDIPIYGYIYDVKSGRLIEVPEATRAGQVVREVSSVR"
     gene            complement(3790..4707)
                     /locus_tag="VF276_12605"
     CDS             complement(3790..4707)
                     /locus_tag="VF276_12605"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEX6607745.1"
                     /translation="MTSSGVIEGTSQASGEEKFRGYANPDILVSTEWLAEHLHDTGLR
                     ILEADEDVLLYDQGHIPGAIKLDWFTELNDPLRRDYLDQTHFEALLSSKGISNDDTLI
                     FYGDKNNWWATYSFWVFQLFGHTKAKILDGGRTKWLAEGRPLETETPSYPATTYKAHE
                     RNDKAIRAFRDQVRDLLDKEGKQLKAGQALVDVRSPAEYSGELLHMANYPQEGALRGG
                     HIPGAANIPWAQAAQEDGTFKPVEQLRALYEGKGVTPDKDVVTYCRIGERSSHTWFVL
                     TYLLGYDKVRNYDGSWTEWGNLVGAPIER"
BASE COUNT          828 a         1712 c         1728 g          908 t
ORIGIN      
        1 ccgcgagatc ccggtcattt tcgtcacggc gaatcatcaa gagcgcgtgt tccgcgagcg
       61 cggcttcagt aactatatcg ccaagccgtt cgacctcgac gaattgctga cccgcgtggc
      121 cgccgcctgc cggcccgagt agctttagct gtaggggccg gcttgcaact ggcctgccct
      181 cgcaccgccg tgatcgtgtt atctgtaggg gccggtttca aaccggctcg gacccgccgc
      241 aatcgcgtgg agtgttccta tgcctgattc cctgatcgac ctgcgcagcg ataccgtcac
      301 tcacccctcg cccgcgatgc gcgaggccat gtacggggcc gaagtcggcg acgatgtgtt
      361 cggcgacgac ccgaccgtga tccgcctgga agcgctggct gccgcaacgc tgggcaagga
      421 agcggccctg ttcgtgccca gcggcaccat gggcaacctg tgtgccgtgc tgacccactg
      481 cgggcgcggc gacgaactca tcgtgggcat ggaggcgcat atccgccacg ccgaggccgg
      541 gggtgcctcg gtgctcggag gcgtggcgat gtggggcatc cccaacgcgc ccgacggcac
      601 actgcccctc gacgccgtcg ccgccgccat ccgcccggtg gaccaccact tcccgcatac
      661 ggcgctgatc tgcatcgaga acacccagaa ccgctgcggc ggcgtgccca ttggccgcga
      721 ctacatggcc gcgctgggcg agatcgccca cagccacggc ctgccgctgc acctcgacgg
      781 cgcgcgcatc ttcaacgccg cggttgcctt cgggatcagc gccgccgacc tggctgcgcc
      841 cgcggacacg gtgatgttct gcctgagcaa ggggctggcc gcgccggtgg ggtccatgct
      901 ctgcgggccg cgcgccttca tcgagcgggc gcggttcaac cgcaagctgg tcggcggcgg
      961 catgcgccag gccgggatca tcgcggcggc gggcatcgtc gccctcaccg agatggtgga
     1021 ccggctggcc gaggaccacg ccaacgcccg ccggctggcc gaggggctgg ccgggctgcc
     1081 gggcctccag gtcgatctca cccgcccgct gaccaacatg gtctacgcca acgtcggcgc
     1141 caccggctgg aacgcggacg aattcaccgc ccgcgccgcg gaggcaggtg tcctgttcgc
     1201 cccgaccgat cccctggtcg tgcgcctggt cacgcactac ggcatcacgg cggcggacat
     1261 cgacactacg ctgagccgct taggtacgat cctgcaagag caagcgacga acgccgggca
     1321 actggccggc gccaccgcct gaacacaaaa gccgggggca cggcgcctgc cgtgcccccg
     1381 tatcctgacg cagattccta ccgcctcttg tgtgtagttg cgcgatttat cgcgccgtac
     1441 tcgttggtct tttgcgtggg cggtccccag gcgctggcgg gaacccgtgt cagcgagtcc
     1501 ccagcaagcg gtagcgctgc agcttcaact ggtaatagat gaagcagccg acgcaaaacc
     1561 cggccagggc catcccggcg gccagggcga ccgtacccgc cagcgcccag cccaccagcg
     1621 gcgcgcccag cagaaacgcc ggctgggcca ggcccagcag caccagggcg atggtgttgt
     1681 tgaagcgcat caagcggcgg tcttcgcgct cggcggtcgg gagttgccgc gcgaacaggc
     1741 gcttgcccac gcgaaagatc aggctcgccc gctggccgat aacgaccgcg ggcagcagga
     1801 tcagaaacag cagcgtggtg agcagcggct gctgcaggat cagacccagg acgatgccca
     1861 cgagcaacac ccagcggttg agccgcacaa tgggcagcgg gatgtcgccg gccgtggggc
     1921 gggccgcgga tgatgaagtt gccatgaacg aatatctcct cctgtgctaa acacgcggcg
     1981 gctctacggg gccactgccc ccacagccag cccggcccgc cgcgcgaggt aatcgacaaa
     2041 gtcctcgaaa ggatcgaagc cgacgatccg cggtgcgtcg gtgacgaagt tggacagggc
     2101 gttgatgtcg tagaggtaca gcccgccgtc gcgggcgctc tccagatact cgaccccgcc
     2161 gatatcgatg ctgttcgcgc ggaagatctg caacaccgct tcgattaccc aggcgggcgg
     2221 ctcggcggct tcgacccgca agggcttctt gaccccgcgt tgacccggcg gcggcaactg
     2281 gcagatgtcg gcggggcaga gattgaagct gtcctgggtg tcgttgtaga tgcggatcgc
     2341 gtagagcagg cggtcgtcga gggcctccac gcgcacgatc gagtcgtggc gcggcggatg
     2401 atactcctgc acgatggccg tgtagtcgag gccgaggatc gagtccagtt cgcgatccgc
     2461 cacggcggcg gccagttcgc cgggtgtgtc gaagcggcgc atcagcgcgc cgctcccgcc
     2521 gacgttcggc ttgacgatga tgggatagtc cagttccgcc gccgcggggg caatctccgc
     2581 gacggcgttg accacccggc tgcggggatg tggcagcccg aggcgcgcca gcaattgcaa
     2641 ctggcgcgcc ttggacagtt ccaggcggta ggcgcgcagg ccgttgacca ccggcacgcc
     2701 gatctcctcc aggtgggcca ggtactgctg ggcatagaag atcgcccgcg cgtgatcgcg
     2761 caggtatgac gacgggctga cccggttgac gaccaggcta tagggtgccg accgggccgc
     2821 cgggtcgaaa cggtgctcct gggccgggat gcgctcatag gccaggcccc ggcgatccag
     2881 cgcggcaaac agcggctgga accattccgg gtgctcgtac aggatgccga tcggctcgtt
     2941 catgtgaatg ctacgtccct tctatctggg tagcgggccg gtttgaaacc ggcccctaca
     3001 ccgcgaaacg ctggtagcca tcgactggcg cgcgggtgcc gtttagcgga cagacgagac
     3061 ttcgcgcacg acctggcccg cacgggtcgc ttcgggcact tcgatcaacc ggccactttt
     3121 cacgtcgtag atgtagccgt aaatcgggat gtcgccgggg accagggcgt gcgcccggat
     3181 gcgccgcacg tcatccagca cgctctgcgc ctggtcgcgg atggtcagga agctaataaa
     3241 ctcggcctcg ttcgagccgg ggccttcgcc gctgtcgtgc cagccgttct cgtccaccga
     3301 tgccgtcttc aggctctgcg ccagcaggcc gcggatgacc tcgtcggtga aggtgagcat
     3361 gccgcaatcg gtatggtgga tgacaaacca ctcgcgggtg cccagcagct tgtaggagat
     3421 caccagcgaa cggatcgcgt cgtcgctggc ccggcccccg gcgttgcgga tcacatgggc
     3481 gtcgccttcg gccagccccg cgtacttggc ggggtcgagc cgcgcgtcca tgcaggtcag
     3541 gatggcaaag tggcgccccg gcggcatggg caactggccc ttatcgccga agttcgccgc
     3601 atattcgttg ttggccgcaa gcacttcatc taagatctgg ctcatttcgt gttcctcccc
     3661 ccggttatgt gttagctgtc agccgtcagc tatcagctat cagtcgtcag cggttagtgg
     3721 tcagcggtgc ggagcacatc accgatcacc gaccactcgt ctcctgacgg ctgatggctg
     3781 atagctcgcc taccgctcga tgggcgcgcc gaccaggttg ccccactcgg tccacgagcc
     3841 gtcgtagttg cgcaccttgt cgtagcccag caggtacgtc agcacgaacc aggtgtggct
     3901 gctgcgctcc ccgatccggc agtaggtcac tacgtccttg tcgggggtca cgcccttgcc
     3961 ctcatacagc gcccgcagtt gctcgacggg tttgaaggtg ccgtcttcct gggcggcctg
     4021 ggcccagggg atgttggccg cgccggggat gtgcccgccg cgcagcgccc cttcctgcgg
     4081 gtagttcgcc atgtgcagca gttccccgct gtactcggcg ggcgagcgca cgtccaccag
     4141 cgcctgcccc gccttcaact gcttgccctc tttgtcgagc aggtcgcgta cctggtcgcg
     4201 gaaggcgcgg atggccttgt cgttgcgctc gtgggctttg taggtggtgg cggggtagct
     4261 gggggtctcg gtctccagcg ggcggccttc ggcgagccac ttggtgcgcc cgccgtcgag
     4321 gatcttggct ttggtgtggc cgaaaagctg gaagacccag aaggaatagg tggcccacca
     4381 gttgttctta tcgccgtaga agatgagggt atcgtcgttg ctgatgccct tgctggacag
     4441 cagcgcctcg aagtgggtct ggtcgaggta gtcgcggcgc agcgggtcgt tgagttcggt
     4501 gaaccagtcg agcttgatcg cgccggggat atggccctga tcgtagagca gcacatcctc
     4561 gtcggcttcg aggatgcgca ggccggtgtc gtgcaggtgc tcggccagcc actcggtcga
     4621 gaccaggata tcggggttcg cgtagccccg gaacttctct tcgccggatg cctgcgaggt
     4681 gccttcaatt acgcctgagc tagtcatggt tggttctcct ccaatactgg tgcctggtac
     4741 tgttcccgaa cgcaccggat ggtgcgcccg gcctgttgtc gcctgcacct cacgccgttg
     4801 tgaggctgtt tgcacacaac aaaaaggggt taccccggcg ggcaacccct gtcatgattc
     4861 tgtgtgcagt caccgcggtc aggcggcagc agatgttacg ttattcggat ctcgctgggc
     4921 gagagcagca ggggcaccgg ggcagaatag ctgggcgtac aacatgcgca cgcgaccagc
     4981 cccgtacagg cgcaacaaca cacggcacac gccaccatcg ggcatccgcc cgcggggcct
     5041 gtgctctgct gctgcatctg cgtgctgctc gtcatcggga tcctcttctc cgcgtctccc
     5101 gcattatatg tgcgcccgcc gtaaggtttc tgtaaggcgg gctacatccg accgggtagc
     5161 ttatgccgaa cctccg
//
LOCUS       DASUYC010000768         4087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1525638, whole genome shotgun sequence.
ACCESSION   DASUYC010000768 DASUYC010000000
VERSION     DASUYC010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1525638"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1630
                     /locus_tag="VF276_12610"
     CDS             <1..1630
                     /locus_tag="VF276_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator SirA"
                     /protein_id="HEX6607746.1"
                     /translation="EQPLLPYEACNLGSINVGRLTVERDGKTDLDWDALREVVHLATR
                     ALEDVIEMNKYPIPEIEEMTTRNRRIGVGLMGWADLLFKLRIPYNSEEATDLAERMMK
                     FIDDEAKNASEQLAAERGPFPNWEESIYGPKGQHWPNAQGVRPLRNSTVSTIAPTGTI
                     SIIAGASGGIEPLFSLAFMRTIMDKDKLLEVNEIFEQVAKDEGFYSPELMEEVAAAGT
                     LAHIEGIPDWVKRVFVTARDVTPEWHARMQAAFQKSTDNAVSKTVNFPPEATEDEVRE
                     VYELAYRLGCKGVTIYRDGSRSEQPMSTVAKKPESGAAPVPATVPLAAAGATATANGN
                     GNGNGHSHDVPEIAAEVREADAQPPVDVAEPHPVLPRPLPDGDIGGWLGRIKSPQGTV
                     RVWVSEVDGQPYETYVVLGKSGSDLMALTEGIGRMMSIALRAGIPVEILIDQLRGIGG
                     SRSVGFGTQRVLSVPDAIAKLLQEHYFPEPDAPAVPASQPVAKAAPAVVQVAPHPRRP
                     ITSQPLGDICPECGNATLMHVEGCKKCPCGHSEC"
     gene            complement(1739..4039)
                     /locus_tag="VF276_12615"
     CDS             complement(1739..4039)
                     /locus_tag="VF276_12615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX6607747.1"
                     /translation="MQNPRQPRDTPIAPPPNTYSTVAPPAGPLAALDAAAESPTTASP
                     SRRIGLPTLIDALTVGVACGVFLLLALYQLHLPGLYYDEAWDAVSTMHIVQGTPVELD
                     RGAHITLFGRQWPLMLDDYQGIISTYLLVPFFLIGGIGVTSIRAFPILSGVIAIVLCY
                     FLARAWFGRGAARIAVLLFALSPSWIFWSRIGVYVVVEIVPFTLGALLAFTAWWRAPR
                     GKNGLLYLGSFLMGLGLATKLLYVWPIVAVLACYILLHGRALWARRRLGLYALRPAWL
                     PASGRVAVPQWRIAGVLALGVVFFCIGSFTFLAFNWQTQGTYLKIRGSATQTEYGTNN
                     TTVMPNLWEEMDRFKVLLDGSYFWFQTAADHPGGPLPVYADVLAPLGFALAAIGLLAA
                     AIWRLPGTQSAAWPARRALGGVLLAFAPALLLCLAAFVRPSSRSALLVAPAALAVLLA
                     GGAAVWLVRAGLRAPATWARPTGAILLGATLGSLATWLLTGQPDGQARGPFDLRVTDL
                     TGVLFWISLVALLFLLGWSAPATRWQRPLVATVGYLAAVLGQSFPTLSGLWPTHLLVM
                     LPLPQILIGAALAQVAGWAPQPRRQAPAAAGTRRPAPPWRAAGVTLAVALMLGSALWS
                     DYRYHRDLGLTGGLFAFSDGIYTLNNYLLKAPDRPVVAAEWGIRRQLEILSQGRLDPE
                     EIFQYGDNSDPQIIAAFDAALNHTLLDVNARYVFTAGTIGPYNRFAEFERVLATRGQV
                     PFLEHTSYMRDGRPIYQVYTARRAIR"
BASE COUNT          725 a         1351 c         1479 g          532 t
ORIGIN      
        1 cgaacagcct ttgctgccct acgaagcgtg caatctcggc tcgatcaacg tcgggcggct
       61 gaccgtcgag cgcgacggca agaccgatct cgattgggat gccttacgcg aagtcgtgca
      121 tctggcgacg cgggcgctcg aagacgtgat cgagatgaat aagtacccga tccccgagat
      181 cgaggagatg accacgcgca accggcgcat cggcgtcggg ctgatgggct gggccgacct
      241 gctgttcaag ctgcgcatcc cgtacaactc ggaggaagcg acggacctgg ccgagcggat
      301 gatgaagttc atcgacgacg aggccaagaa cgcgtcggag caactggctg ccgagcgcgg
      361 gccgttcccc aactgggaag agagcatcta cgggccgaag ggccagcact ggcccaacgc
      421 ccagggcgtg cgcccgctgc gcaactcgac cgtcagcacc atcgccccca ccggcaccat
      481 ctcgatcatc gccggggcgt cgggcggcat cgagccgctc ttctcgctgg ccttcatgcg
      541 cacgatcatg gacaaggaca agctgctgga ggtcaacgaa atcttcgagc aggtggcgaa
      601 ggacgaaggc ttctacagcc ccgagttgat ggaagaagtc gcggcggcgg gcacactggc
      661 ccatatcgag ggcatccccg actgggtgaa gcgcgtcttc gtcaccgcgc gcgacgtgac
      721 gccggagtgg cacgcgcgca tgcaggccgc gttccagaaa tcgacagaca acgcggtctc
      781 aaagacagtc aatttcccgc cggaagccac cgaggacgaa gtccgcgagg tctacgaact
      841 ggcctatcgc ctgggctgca agggcgtgac catctaccgc gacggcagcc ggtccgagca
      901 gccgatgagc accgtcgcca agaagccgga aagcggcgcg gccccggtgc cggccaccgt
      961 gccgctggcc gccgcaggcg cgacggcgac ggccaacggc aacgggaacg gcaacgggca
     1021 cagccatgat gtgccggaga tcgcggcgga agtgcgcgaa gccgacgccc agccgcccgt
     1081 ggatgtggcc gagccgcacc cggtgctacc gcgcccgctg cccgacggcg atatcggcgg
     1141 ctggctgggc cgcatcaaga gcccgcaggg caccgtgcgc gtctgggtga gcgaagtgga
     1201 cggccagccc tacgagacct atgtcgtgct cggcaagtcg gggtccgacc tgatggcgct
     1261 gaccgagggc atcgggcgca tgatgtccat cgccctgcgg gcgggcatcc cggtcgagat
     1321 cctgatcgac cagttgcgcg gcatcggcgg cagccggtcg gtcgggttcg gcacccagcg
     1381 cgtgctctcc gtgcctgacg ccatcgccaa gctgttgcag gagcactact tcccggagcc
     1441 ggacgctcca gcggtccccg cgtcccagcc ggtcgccaag gcggccccgg cggtggtgca
     1501 ggtggctccg catccgcgcc ggcccatcac cagccagccg ctgggcgaca tctgcccgga
     1561 gtgcggcaac gccacgctga tgcacgtcga aggctgcaag aagtgcccct gcggccacag
     1621 cgagtgctaa cgtagctgtc agccgtcagc tatcagccgt cagctatcag gtaattagga
     1681 agggcgcccg gcgtgggcgg cccttcctct ttttgtgagt cacgcaggta ggcagcgatt
     1741 agcggatcgc gcggcgggcg gtgtagacct ggtagatggg gcggccgtcg cgcatatagg
     1801 acgtgtgctc caggaagggc acctggccgc gcgttgccag cacgcgctcg aactcggcga
     1861 agcggttata ggggccgatg gtgcccgcgg tgaagacgta gcgggcgttc acgtccagca
     1921 gcgtgtggtt cagggccgcg tcgaaggcgg cgatgatctg cgggtccgag ttgtcgccgt
     1981 actggaagat ctcctccggg tcgagccggc cctggctcag gatctctaac tggcggcgga
     2041 tgccccactc ggcggcgacc acggggcggt cgggcgcttt gagcaggtag ttgttcagcg
     2101 tgtagatgcc gtcggagaag gcgaacaggc cgccggtcag ccccaggtcg cggtgatagc
     2161 ggtaatcgga ccagagcgcg ctgcccagca tcagcgccac ggcgagcgtg accccggcgg
     2221 ctcgccaggg cggcgcgggg cggcgggtgc ctgcggcggc gggcgcttgc ctacggggtt
     2281 gcggcgccca tccggccacc tgcgccagcg ccgcgccgat gaggatctgc ggcaggggca
     2341 gcatgaccag caggtgcgtg ggccagaggc cggagagggt ggggaagctc tggccgagta
     2401 ccgccgccag gtagcccacc gtggcgacca gcgggcgctg ccagcgggtg gcgggggcgc
     2461 tccagcccag caggaacaac agcgccacca ggctgatcca gaacagcact ccggtgagat
     2521 cggtcacacg caggtcgaac ggcccgcgtg cctgcccgtc gggctggccg gtcagcagcc
     2581 aggtggcgag gctgccgagc gtggcgccga gcaggatcgc gcccgtcggg cgggcccagg
     2641 tcgccggggc gcgcagcccg gcacgcacga gccacaccgc cgcgccgccg gccagcagca
     2701 cggccagcgc cgcgggcgcc accagcaacg ccgagcggct gctgggccgc acgaacgcag
     2761 ccaggcacag cagcagggcg ggcgcgaagg ccagcagcac gccgcctaac gcgcgccggg
     2821 ccggccatgc ggcggattgc gtacccggca gccgccagat cgccgccgcc agcaagccga
     2881 tcgcggccag cgcgaacccc agcggcgcca gcacatcggc atacaccggc agcgggccgc
     2941 ccggatggtc ggcggcggtc tggaaccaga agtagctgcc gtcgagcagc accttgaagc
     3001 ggtccatttc ttcccacagg ttcggcatga ccgtggtgtt gttggtgccg tactcggtct
     3061 gcgtggcgct gccgcggatc ttgagatagg tgccctgggt ttgccagttg aaggccagga
     3121 aagtgaacga cccgatacag aagaacacca cgcccagcgc caggaccccg gcgatgcgcc
     3181 attggggcac cgcgacccgc ccgctggccg gcagccaggc cgggcgcagg gcgtacaggc
     3241 ccagccggcg ccgcgcccag agcgcgcgcc cgtgcagcag gatatagcag gccagcaccg
     3301 cgacaatggg ccagacatag agcagcttgg tcgccaggcc caggcccatg aggaagctgc
     3361 ccaggtagag caggccgttc ttgccgcgcg gcgcgcgcca ccaggcggtg aacgccagca
     3421 gcgcgcccag cgtgaacggc acgatctcga cgaccacgta gaccccgatg cggctccaga
     3481 agatccagga cggcgagagg gcaaagagca gcacggcgat gcgcgccgcg ccccgcccga
     3541 accaggcgcg ggccaggaag tagcagagca cgatggcgat aacgccgctc aggatcggga
     3601 acgcccggat cgatgtgacg ccgatgccgc cgatcaggaa gaagggcacc agcaggtagg
     3661 tgctgatgat gccctggtag tcgtcgagca tgagcggcca ctgccggcca aacaacgtga
     3721 tatgcgcgcc gcgatccagt tccaccgggg tgccctgcac gatgtgcatc gtcgagacgg
     3781 cgtcccaggc ttcgtcataa tagaggcccg gcagatggag ttggtagagc gccagcagca
     3841 ggaacacgcc acaggccacg ccgaccgtga gcgcgtcgat cagcgtgggc aggccgatgc
     3901 gccgggatgg gctcgcggtg gtcgggctct cggcagccgc gtccagcgcg gctagcgggc
     3961 cggcgggcgg ggcgacggtg ctatatgtgt tgggtggcgg cgctatggga gtgtcgcggg
     4021 gttgcctcgg attttgcacg caaatttcct aaattccagg gttgccgccc ggctcctgga
     4081 ggcggcg
//
LOCUS       DASUYC010000769         2229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_119585,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000769 DASUYC010000000
VERSION     DASUYC010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119585"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            461..>2229
                     /locus_tag="VF276_12620"
     CDS             461..>2229
                     /locus_tag="VF276_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018237877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX6607748.1"
                     /translation="MMRWIVGSSLKLRYMVLAVAAAAMVLGIIRLRDAPVDVFPEFAP
                     PYVEIQTEALGLSAAEVEQLVTVPMEELFNGVPWMQTMRSESIPGLSSIVLIFQPGTD
                     LMRARALVQERLTQAQGLPTKNVSKPPVMLQPLSSTSRVMQIGLSSKDLSLIEMSVLT
                     RWTIRPRLMGVPGVANVAVWGARQRQLQVQVDPERLRAHGVKLDQIIRTAGDALWVSP
                     LSFLNASTPGITGGFIDTPNQRLGVRHFLPISSPDDLAQVTVAGATMPLGDVATVVEN
                     HQPLIGDAVINDGQGLLLVVEKFPWANTLEVTRGVEDALAALQPGLPGLAMDSTIFRP
                     ATFIEMALENLTLSLIIGSLLLVLVVGAFLFDWRTALISLVAIPLSLVAGALVLHLSG
                     AGINTMILAGFVIAVGVVVDDAIIDIENIARRLRQNRQEGSPKSTASIVLEASLEVRG
                     SIIFATLIIVLALLPVFFLEGLSGAFFQPLALSYVLAVLASMLVALTVTPALALILLG
                     GDHAARRESPLARWLQRGYERVLARLIRSPRPAYLAVAVMLVLGLAVLPFLGQSLLPS
                     FKENDLLVDWVGAPGTSHPEMTR"
BASE COUNT          334 a          716 c          755 g          424 t
ORIGIN      
        1 gagcttcagg tgaggtgctc gaaggccgtt ttaccgcccc aacgtcctgc cgcccggcca
       61 cctgttccac gatcacactg gcaccgccgg caacaacctg atccagcaac caccaagcca
      121 aagttcatat ttcgtccttg agcgcaggtc gcgcttatcg gcaacccaat tcgcaaaacg
      181 cgccgtcggg catgcgtagt gccatcctgg ctaaccgcga accggcgctc gccataccgc
      241 tttaagtcgt cggtggccgt tcacggcatc accgcatagg gatcgtgttg cctttccata
      301 tggcatccgg agtattggcg ctgggggctg aatatcgtgc gcccgtaatt ctagcgactg
      361 gaataaggga ccaatatcct aggacgggcc gggcggagcc gacggttgct ccgtgccacg
      421 gcgcgggttg gaacatagcg acatagggag ggctccagcc atgatgcgtt ggatcgtcgg
      481 atcaagcctg aagttgcgat acatggtgct ggccgtcgcc gcggcggcga tggtcctggg
      541 catcatccgg ctgcgcgacg cgccggtgga tgtgttcccc gagtttgcgc cgccctacgt
      601 ggagatccag accgaagcgc tgggtctgtc cgccgccgaa gtggaacaac tggtgacggt
      661 gcccatggag gagttgttca acggcgtgcc ctggatgcag accatgcgct ccgagtcgat
      721 tccggggctg tcgtccatcg tcctcatctt ccagcccggc accgacctga tgcgggcgcg
      781 ggcgctggtg caagagcggc tgacccaggc tcagggcctg ccgacgaaga acgtgtccaa
      841 gccgccggtc atgctccagc ccctgtcgtc caccagccgg gtgatgcaga tcgggctgtc
      901 ctccaaggac ctgtcgctga tcgagatgtc ggtgctcacc cggtggacga tccgcccgcg
      961 cctgatgggc gtgcccggcg tggcgaacgt ggccgtctgg ggcgcgcggc agcggcaact
     1021 ccaggtgcag gtcgatcccg aacggttgcg ggcgcatggg gttaagctcg atcagatcat
     1081 ccgcaccgcc ggggacgcgc tgtgggtgtc gccgttaagc ttcttgaacg cttcgacgcc
     1141 gggtatcacc ggcgggttca tcgacacgcc caaccagcgg ctcggcgtgc ggcacttcct
     1201 gcccatttcc tcgcccgacg acctggccca ggtgacggtg gcgggcgcta cgatgccgct
     1261 gggcgacgtg gcgacggtgg tggagaacca ccagccgctc atcggggacg cggtcatcaa
     1321 cgatgggcag ggcctgctgc tcgtcgtgga gaagttcccc tgggccaaca cgctggaggt
     1381 gacgcgcggg gtggaagacg ccctcgccgc cttgcaaccc ggcctgccgg gtctggctat
     1441 ggactcgacc atcttccgcc cggcgacctt catcgagatg gcgctcgaaa atctgactct
     1501 gtcgctgatc atcgggtcgc tgctgctggt gctcgtggtg ggtgcgttcc tcttcgactg
     1561 gcgcacggca ctgatcagcc tggtggcgat cccgctgtcg ctggtggcgg gagcgctggt
     1621 gctccacctg agcggggccg ggatcaacac catgatcctg gcggggttcg tgatcgccgt
     1681 gggggtggtg gtcgacgacg ccatcatcga catcgagaac atcgcccggc ggctacggca
     1741 gaatcgccag gagggcagcc ccaaatcgac ggcgagcatc gttctggaag cctcgctgga
     1801 ggtgcgcggc tcgatcatct ttgcgacgct gatcatcgtg ctggcgttgc tgccggtctt
     1861 cttcctggag gggttgtcgg gcgcgttttt ccagccgctg gcgctgtcct atgtgctggc
     1921 ggtgctggcc tcgatgctgg tggcgctgac cgtcaccccg gccctggccc tgatcctgtt
     1981 gggcggcgat cacgccgcgc gccgcgaatc accgctggcc cggtggttac agcgcggcta
     2041 tgagcgggtc ctggcccggc tgatccgcag cccgcgcccg gcgtacctgg ccgtggcggt
     2101 catgctggtg ttggggctcg ccgtgttgcc cttcctgggc cagtcgctgc tgccctcgtt
     2161 caaggagaac gacctgctgg tcgattgggt cggcgcgccc ggcacgtctc accccgaaat
     2221 gacgcgcat
//
LOCUS       DASUYC010000770         2578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_582451,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000770 DASUYC010000000
VERSION     DASUYC010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582451"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /gene="rlmB"
                     /locus_tag="VF276_12625"
     CDS             <1..771
                     /gene="rlmB"
                     /locus_tag="VF276_12625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEX6607749.1"
                     /translation="RGGKGAPPGRPGAGRPDARGRAIPADETRSERRTVSLAGRAPLE
                     EIMTLARSKGVPVERVPGERLDKLTNGANHQGVAAVAADYHYLAWGDMLARVEKAGPR
                     ALVLVLDSLQDPQNFGTLLRTAEAVGVTGVIMPEHHAVGVTPAVCNASSGAVEYLHIT
                     QVVNLARTIEELKAANVWCYALAGDDAAAAYDSVDLQRPLALVVGSEGVGVSALVRKR
                     CDGTLALPMHGRIESLNAAVAGSVVLYEVLRQRGEAKE"
     gene            complement(853..1782)
                     /locus_tag="VF276_12630"
     CDS             complement(853..1782)
                     /locus_tag="VF276_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607750.1"
                     /translation="MAPRLVKRARADFGRPLAVIFAAYTGATGCICCAYNQRKDAVNQ
                     EEVAMRPRKTTWIACVVVLALLGAAVAALVFVATYTPLDRQIGLTLRADTTASPATRA
                     NDLQRTRDVIAARVAQLHFKDWRVETHGVDGIVIKLPNDVPLGDVMRLTYSPGELELI
                     AAGSAPIPGGMLVSTDRGAPTDVQLKIARQSGPYAGGHVYPVVATDADIDRDRVTIEN
                     STQTGTSRVGITLQAQAAQTLRQFTRDHPGQYLGILLDKRVLACPVIVMELSGNVLTV
                     DHLSPEDAQQLAVQLRSGPLPAPLSDTIEMSQP"
     gene            1858..1930
                     /locus_tag="VF276_12635"
     tRNA            1858..1930
                     /locus_tag="VF276_12635"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1891..1893,aa:Thr,seq:tgt)
     gene            1945..2028
                     /locus_tag="VF276_12640"
     tRNA            1945..2028
                     /locus_tag="VF276_12640"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1979..1981,aa:Tyr,seq:gta)
     gene            2035..2107
                     /locus_tag="VF276_12645"
     tRNA            2035..2107
                     /locus_tag="VF276_12645"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2068..2070,aa:Thr,seq:ggt)
     gene            2219..>2578
                     /locus_tag="VF276_12650"
     CDS             2219..>2578
                     /locus_tag="VF276_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX6607751.1"
                     /translation="MAKKRFERNKPHLNVGTIGHVDHGKTTLTAAITKVMSLQGKADF
                     VDYAQIDNAPEERERGITIAIRHVEYETDKRHYAHVDCPGHADYIKNMITGAAQMDGA
                     ILVVSAPDGPMPQTREHI"
BASE COUNT          450 a          854 c          845 g          429 t
ORIGIN      
        1 cggggcggca agggggcgcc gccggggcgg cccggcgctg ggcgccccga cgcacgcggg
       61 cgggcgatcc ccgccgacga aacgcggtcc gaacggcgga ccgtctccct ggccgggcgc
      121 gcgccgctgg aggagatcat gaccctggcc cgcagcaagg gcgtgcccgt ggagcgcgtg
      181 cccggcgagc gcctggataa gctgaccaac ggcgccaacc accagggcgt ggccgccgtg
      241 gccgccgact accactatct cgcctggggc gatatgctgg cgcgggtgga gaaagccgga
      301 ccgcgggcgc tggtcctggt cctcgacagc ttgcaggacc cgcagaactt cggcaccctg
      361 ctgcgcaccg ccgaggccgt gggcgtgacc ggcgtgatca tgcccgagca ccacgccgtg
      421 ggtgtgaccc ccgccgtgtg caacgcgtcg tcgggcgccg tcgagtatct gcatatcacc
      481 caggtcgtca acctggcgcg caccatcgag gaactgaagg ccgccaacgt ctggtgctac
      541 gcgctggccg gggatgacgc cgccgcggcc tatgacagcg tggatttgca gcgccccctg
      601 gccctggtcg tcggctcgga gggggttggc gttagcgccc tggtgcgcaa gcgctgcgac
      661 ggcacgctgg ccctgccaat gcacgggcgc atcgagtcgc tcaacgccgc cgtggccggg
      721 tcggtggtgc tctacgaggt gctgcgccag cgcggcgagg ccaaggagta ggcagccgcc
      781 gcgatttcgc ggtgtagggg ccggtttcaa accggcctgt cgcctctcag aatcagtgcg
      841 gtgggcgagt agttacggct ggctcatctc gatggtgtcg ctgagcggcg cgggcagtgg
      901 accggatcgc aactgaacgg ccagttgttg cgcgtcctcc ggactcaggt gatcgacggt
      961 cagcacgtta ccggacagtt ccatgacgat caccgggcac gcgaggacgc gcttgtccag
     1021 caagataccc agatactgtc cagggtgatc gcgggtgaat tggcgtagcg tctgcgcggc
     1081 ctgcgcctga agggtaattc ctacccgcga tgtaccggtt tgcgtgctgt tctcgatagt
     1141 caccctatcg cggtcgatgt ccgcatcggt ggcgacgacc ggatagacgt gccctccggc
     1201 gtaggggccg ctctgcctgg cgatcttcaa ctgcacatcc gtcggtgcgc cgcgatcagt
     1261 actcacgagc atgccgccgg ggatcggcgc gctgccggcg gcgattagct ccagttctcc
     1321 cggcgagtac gtcaggcgca tgacatcgcc aagcggcacg tcgttaggca gtttgatcac
     1381 gatgccgtcc actccgtgcg tttcgacccg ccagtcctta aagtgcaact gcgccacgcg
     1441 agcggcaatg acatcccgcg tgcgttgcag atcgttcgcg cgggttgccg ggcttgccgt
     1501 ggtatccgcg cgaagcgtca gcccaatctg acggtccagc ggcgtatagg tagcgacgaa
     1561 tacgagtgcg gcgaccgccg cgccgaggag ggcgagtacc acaacgcagg caatccaggt
     1621 cgttttccgc gggcgcatgg caacttcctc ctgatttacg gcatctttcc tctggttata
     1681 cgcacagcag atgcacccgg ttgcgccggt gtacgcggca aaaatcaccg ccaaaggacg
     1741 gccaaaatcg gcccgcgcac gcttgacaag ccggggcgcc atcgtgtata atccgttgca
     1801 cgctgcaaaa cggcgtgcgg gagagcagcg ggtcgggcct ccgtccaaca cactcgtgcc
     1861 gacgtagctc aattggcaga gcccctgttt tgtaaacagg ctgttgcggg ttcgagtccc
     1921 accgtcggct cccttctata tcgcgggcag gtgccggagt ggccaatcgg aactgactgt
     1981 aaatcagtcg ggcttcgccc tacggaggtt cgaatccttc cctgcccacc ccgggcccac
     2041 gtagctcagt tggtagagca cgttcttggt aagaacgggg tcaccagttc aagtctggtc
     2101 gtgggctccg tacacagcta gcaggcccca gctatctcct gatagcgtgg ggctttgctt
     2161 cgtatcccgc ccgccggtcg cccggcgcga cgtagactat taaaggagag cagttagagt
     2221 ggctaagaag agattcgagc gcaacaagcc gcacctcaac gtgggcacca tcggccacgt
     2281 cgaccacggc aagaccaccc tcaccgccgc catcaccaag gtgatgagcc tgcagggcaa
     2341 ggccgatttc gtcgattacg cccagatcga taacgccccc gaagagcgcg agcgcggcat
     2401 caccatcgcc atccgccatg tcgagtacga gaccgacaag cgccactacg cccatgtgga
     2461 ctgccccggc cacgccgact acatcaagaa catgatcacc ggcgccgccc agatggatgg
     2521 cgccatcctg gtcgtgtcgg cccccgacgg ccccatgccc cagacccgcg agcacatc
//
LOCUS       DASUYC010000771         2922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_582469,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000771 DASUYC010000000
VERSION     DASUYC010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582469"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..1305
                     /gene="hutI"
                     /locus_tag="VF276_12655"
     CDS             4..1305
                     /gene="hutI"
                     /locus_tag="VF276_12655"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEX6607752.1"
                     /translation="MPPTAATPPAAPAELIIAHAAELLTMAGPAEARRGPQQAEWGLI
                     PDGAVAISGDTIRAVGPSAAILAAHAGPDTRIVDAAGRVVTPGLVDAHTHLVFAGTRE
                     HEYEQRLQGAGYLEILAAGGGIMSTVRATRAASAEELAAGMVPRLRTMLAHGTTSVEV
                     KSGYGLSTEDELKSLHAIRIAGTGRAPGQPGAALPRLCPTFLGAHAVPEEYRDQPDAY
                     VDLLVEETLPAVAAAGLAESCDVFCDAGAFDAAQARRVLEAAQAHGLPVRLHANEFER
                     IGAAALAADLHALSADHLLVLDPEEIAALAAAGVVATLLPGTGLGLGHYAPARALIAA
                     GVPVALATDCNPGTCLCENLALMIALACTAMKMTPAEALVAATINSAHALGPRWAAQV
                     GSLEPGKRADLVIWDVPNYRHIPYHFGVNLVGQVVLGGVLV"
     gene            complement(1378..2433)
                     /locus_tag="VF276_12660"
     CDS             complement(1378..2433)
                     /locus_tag="VF276_12660"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6607753.1"
                     /translation="MTTTEVEISLFDPNTATGAEWIAFNTFKNAALAEREPDDPPRTV
                     EQRQVDLRNIPPVFQVRHWLAWDGDHRTVLGVAALILIEMEENRHVAQFELDVLPAHR
                     RRGLGSRLLGLLADQAAAANRRLLIIETHGAVPAGDAFMAHVGAHLALESHINQLDLA
                     QLDRDLLHTWQSDARHAGFTLGLWEGPYPEADLDAIVELVKAANDAPRGDLDVNDFEW
                     TAEQLRAQEASRQARGVERWTMYVRETATGELAGYTEVFWDPAKPALLEQGFTAVWPR
                     YRGHALGRWLKAAMLDKVLRERPQVRYVRTGNADSNAAMLKINTDLGFRPYHSDNVWQ
                     IEVNRVKEYLATRGGVS"
BASE COUNT          391 a         1024 c         1030 g          477 t
ORIGIN      
        1 cccatgccgc caaccgccgc gaccccgccc gccgccccgg cggagctgat tatcgcccat
       61 gccgccgaac tgctgaccat ggccggtccg gcggaggcgc ggcgcgggcc gcaacaggcc
      121 gagtgggggc tgatcccgga cggcgccgtg gcgatcagcg gcgacacgat ccgggcggtg
      181 gggccgagcg ccgccatcct ggccgcccat gccgggccgg acacgcggat cgtggacgcg
      241 gccgggcgcg tcgtgacgcc ggggctggtc gatgcccaca cgcacctggt gttcgccggg
      301 acgcgcgagc atgagtatga acagcggttg cagggtgcgg gctacctgga gatcctggcg
      361 gcgggcggcg ggatcatgtc caccgtgcgc gccacgcgcg cggcctccgc cgaggaactg
      421 gccgcgggca tggtgccgcg cctgcgcacg atgctggccc acggcacgac cagcgtcgag
      481 gtcaagagcg gctacggcct cagtaccgag gacgaactga agagcctgca cgcgatccgc
      541 atcgcgggca cgggccgcgc gccggggcag cctggcgcgg ccctgccccg cctctgcccg
      601 acgttcctgg gcgcccacgc cgtgccggag gagtatcgcg accagcctga tgcctatgtt
      661 gatctgctgg tggaggagac gctgcccgcc gtagcggctg ccgggctggc cgagtcgtgc
      721 gatgtgttct gcgacgcggg cgcctttgac gcggcgcagg cccggcgcgt gctcgaagcc
      781 gcccaggccc acggcctgcc cgtgcgcctg cacgccaacg agttcgagcg catcggcgcc
      841 gccgccctgg ccgccgatct gcacgccctc tccgccgacc acctgctggt gctggacccg
      901 gaggagatcg ccgcgctggc cgcggcgggg gtggtcgcca cgctgctgcc gggcaccggc
      961 ctgggcctgg gccactacgc cccggcgcgg gcgctgatcg ccgccggggt gccggtggcc
     1021 ctcgccaccg actgcaaccc cggcacctgc ctctgcgaga acctggcgct gatgatcgcc
     1081 ctggcctgca ccgccatgaa gatgaccccg gcggaggccc tggtcgccgc cacgatcaac
     1141 agcgcccatg ccctggggcc gcgctgggcg gcgcaggtgg gcagcctgga acccggcaag
     1201 cgggccgacc tggtgatctg ggacgtgccc aattaccgcc acatccccta tcacttcggc
     1261 gtgaacctgg tgggccaggt ggtgcttggc ggcgtcctcg tctagccgcc cgcggattcc
     1321 gctgcgtgac gggccgggtt ggaaccggcc cctacctcgc gcaatcgccg ggaccggcta
     1381 cgagactcca ccacgcgtcg cgaggtactc ctttacgcga ttgacctcga tctgccagac
     1441 gttgtccgag tggtagggcc ggaagcccag gtcggtgttg atcttgagca tggcggcgtt
     1501 ggagtcggcg ttaccggtgc gcacgtagcg cacctgcggg cgctcgcgca gcaccttgtc
     1561 gagcatggcc gccttgagcc accggcccag cgcatggccc cggtagcgcg gccagacggc
     1621 ggtaaagccc tgctccagca gggcgggctt ggccgggtcc cagaacactt ccgtgtagcc
     1681 agccagttcg ccggtggccg tctcgcgcac atacatcgtc cagcgttcga cgccgcgcgc
     1741 ctgccgggac gcctcttgcg cgcgtagttg ctcggcggtc cactcgaaat cgttgacatc
     1801 gagatcgccc cgcggcgcgt cgttggccgc cttgaccagt tcgacaatcg cgtcgagatc
     1861 ggcctccgga taggggcctt cccagaggcc gagcgtgaat ccggcgtgcc gggcgtcgct
     1921 ttgccaggtg tgcagcaggt cgcggtcgag ttgcgccagg tcaagctggt tgatgtggct
     1981 ttcgagcgcg agatgcgctc ccacgtgggc catgaaggcg tccccggcgg gcaccgcgcc
     2041 gtgagtttcg atgatcagca gcctgcggtt ggccgccgcc gcctgatcgg ccagcaggcc
     2101 cagcagccgg ctgcccagcc cccggcggcg gtgggccggc agcacgtcca gttcaaactg
     2161 ggccacgtgg cggttttctt ccatctcgat caggatgagc gcggccacgc ccagcactgt
     2221 gcggtgatcg ccgtcccagg ccagccagtg gcgcacctgg aacaccggcg ggatgttgcg
     2281 caggtccacc tggcgctgct cgacggtgcg cggcgggtcg tccggttcgc gctcggctaa
     2341 ggccgcgttc ttgaacgtgt tgaaggcgat ccactcggcg ccggtcgctg tgttgggatc
     2401 gaagaggctg atctctacct ctgttgtcgt catcgttgtt actccctctc tggaacggac
     2461 cctgctcacg ccgcgtggcg gcagacccgc gccgccctgg cgcggatcaa cacagtgatt
     2521 acgcccggat gggcggccag gttacgctgc ttgccgcgtt tggtacgtac tcctatccgg
     2581 tacaacgctt cgatcatggc aacgcgcgcc cggcttgggc tataatggcg ggtggtgcgg
     2641 gcaggcgggt gaatcccggt ataggctttt actgatagga ttgagttggc gatttcgttc
     2701 tcggagcgtc cagtacatac tatgcgcccc gtctccgccg tgatgggatg ggcggcgtga
     2761 gcatgccgcc gttgcggggg cgggcgctgc cgccctgggc gccgcatctc ctggcgctgc
     2821 tgggctattt cgcgctggcc gtgctggtta gttggcccac catcctctat ttcgacagcc
     2881 gcatcaccgg caacctcatc cccgaccgcg accagaacct gt
//
LOCUS       DASUYC010000772         3079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1274864, whole genome shotgun sequence.
ACCESSION   DASUYC010000772 DASUYC010000000
VERSION     DASUYC010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274864"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="VF276_12665"
     CDS             <1..850
                     /locus_tag="VF276_12665"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX6607754.1"
                     /translation="QLKLIEIVHVLAGADLPLVLLSIAIYFPFVLIKSERWRLLAARL
                     GVPMTAGEAWRLYAVGLGAGAFTPGQAGDLVKAWALQSRGRRLGAAVASSILDRLFDL
                     AGLAVLAALGIAVFGREFAGGWGPAVALLGLGAVGAVVVFARRDMLLRLVMRRAPADA
                     PPATLDTRSMLLAGGWTVLSFVVSTLRVWLLALAIGLTLDPATVGGLVGLTTVAALIP
                     VSVSGVGTRDAMMVVLFSRLQQPAAAAVALSTLILGLNVANAVFGYAVWWVEARTGHP
                     IPQEAP"
     gene            852..2729
                     /locus_tag="VF276_12670"
     CDS             852..2729
                     /locus_tag="VF276_12670"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX6607755.1"
                     /translation="MNLHLDPCTLTALLATLGALVLVGVGAGLLGAGVLSRVPAWTGP
                     ASGPPANHNLLVIIGGAVAVLAAGGLGYLAENSCAVVSAQCNQALSGSTLNLIANPAF
                     AAGRDKTSPLDGWQVQGTLGTDAPQGWIAPAPNTDVDSARIWVRPGAHYCFAVDMAGA
                     GQGQVVVAWDDIHLDELDTTVVPPPGVGRDLRGEVVAPEGAVYARFRLHTIDGAPRYH
                     APVLAEGGVRLEQWPNGAAAALAFSFDWESTMGSPIHSRTEHDLAYARQRGRNMRQGA
                     DILLDYFQQNHIQATFYANGYNLLDGNTAREKFAGDPTYKYGASQGWHTTWWITNTWY
                     SDDPYGTVQSDPDWYFGDQTDRLVAAGQEIGNHTFGHIYGRATKRAQLQADLDSWSAA
                     AQARKLPPVHAFAFPFRSSNDLVPGFYDVLAAHGIDSVTRLFLPDLKDRYVLYGSSVY
                     TAGLMTVVPDWLLGAEGPDLSDGGTAEVNGIAEAQSIIAENIARRGFTTFWTHPEQLV
                     NADVRDSWRETIAAAAAARDKGDLWIAPVTTITHYWRDLAQVSVNSATNGDKLAITVS
                     SKAAEPLEGVTLTLPRPAAEVRIGGAVVKQPRPTQVILPRLDPNAPIQVEVTLAAGGS
                     R"
     gene            2726..>3079
                     /locus_tag="VF276_12675"
     CDS             2726..>3079
                     /locus_tag="VF276_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607756.1"
                     /translation="MIQAASITLALMAAVGGLILGGGLSAALLLGLRRRGATLNGRRA
                     GIIAGLGALVLAVIAGWGGLVFGGDAPIPAACQGVQAQGRTNLIANPAFAPGPDGTLA
                     SWTITSTLASGAPGGW"
BASE COUNT          418 a         1092 c         1116 g          453 t
ORIGIN      
        1 gcaactcaaa ctgattgaga tcgtccacgt cctggccggg gccgacctgc cgctggtgct
       61 gctgtcgatc gccatctact tcccgttcgt gctgatcaag tcggagcgct ggcggttgct
      121 ggcggcgcgg ctgggcgtgc ccatgacggc gggcgaggcg tggcggctct acgcagtggg
      181 gctgggggcg ggcgccttca cgccggggca ggccggcgac ctggtcaaag cctgggcgct
      241 gcaaagccgc gggcggcggc tgggcgcggc ggtcgccagc agcatcctcg accggctgtt
      301 cgatctggcc gggctggcgg tgctggccgc gctgggcatc gccgtgtttg gccgcgagtt
      361 cgcgggcgga tggggccccg ccgtggcgct gctcggtctg ggcgccgtgg gcgcggtggt
      421 ggtcttcgcc cgccgcgaca tgctgctgcg cctggtcatg cgccgcgcgc ccgccgatgc
      481 gcccccggcc acgctcgaca cgcgcagtat gctgctggcg ggcggctgga cagtgctgag
      541 cttcgtcgtc agcaccctgc gcgtctggct gctcgccctc gccatcgggc tgacgctgga
      601 cccggccacg gtcggcggcc tggtcggcct gaccacggtg gcggcgctga tcccggtcag
      661 cgtcagcggg gtcggcaccc gcgacgcgat gatggtcgtg ttatttagcc ggctgcaaca
      721 gccggcggcg gcggcggtcg ccctctcgac cctgatcctg gggttgaacg tggcgaacgc
      781 cgtctttggc tacgcggtct ggtgggtgga ggcgcggacc ggccacccca ttccgcagga
      841 ggcaccgtaa cttgaacttg cacctggatc cctgcacgct caccgccctg ctggcgaccc
      901 tcggggcgct ggtcctggtc ggcgtggggg caggactcct gggcgccggg gtgttgagcc
      961 gggttcccgc gtggaccggc ccggcatcgg gtccgccggc gaaccataat ctgctggtca
     1021 tcatcggcgg cgccgtggcc gtgctcgccg cgggcggcct cggctacctg gcggagaata
     1081 gctgcgccgt ggtctcggcc cagtgcaacc aggcgctgag cggatcgact ctcaatctga
     1141 tcgccaaccc cgcgttcgcc gccgggcgcg acaaaaccag cccgctggat ggctggcagg
     1201 tgcagggcac gctgggtacg gacgcgccgc agggctggat cgcacccgcg ccaaacaccg
     1261 atgtcgactc ggcgcgcatc tgggtgcggc ccggcgcgca ctattgcttc gccgtggaca
     1321 tggccggggc gggccagggc caggtggtcg tggcctggga cgacatccac ctggacgaac
     1381 tggacaccac cgtcgtgccg ccgccgggcg tgggccgcga cctgcgcggc gaggtggtgg
     1441 cgccggaggg cgcggtctat gcccgcttcc gcctgcacac catcgacggt gcaccccgct
     1501 atcacgcgcc ggtgctggcc gagggcggcg tgcgcctgga gcagtggccc aacggcgccg
     1561 ccgccgcgct cgccttctcg ttcgactggg aaagcaccat gggcagcccg atccactcgc
     1621 gcaccgagca cgacctggcc tatgcccgcc agcggggccg caacatgcgc cagggcgcgg
     1681 atatcctgct cgactatttc cagcagaacc acatccaggc gaccttctac gcgaacggct
     1741 acaatctgct cgacggcaac accgcgcgcg agaagttcgc cggcgacccg acctacaagt
     1801 atggggcgag ccagggctgg cataccacct ggtggatcac taacacgtgg tacagcgacg
     1861 acccctacgg caccgtgcag agcgaccccg actggtattt cggggaccag accgaccggc
     1921 tggtggcggc gggccaggag atcggcaacc acaccttcgg ccacatctat gggcgggcca
     1981 ccaagcgcgc gcagttgcag gccgacctgg actcctggtc ggcggcggcg caggcgcgca
     2041 aactgccgcc ggtccacgcc ttcgccttcc ccttccgctc gtcgaacgac ctggtgcccg
     2101 gcttctacga tgtgctggcg gcgcacggca tcgactcggt gacgcgcctc ttcctgcccg
     2161 acctgaaaga ccgctacgtg ctctacggat cgtccgtcta cacggcgggg ctgatgaccg
     2221 tggtgcccga ctggctgctg ggcgcggaag ggcccgacct gagcgacggc ggcaccgccg
     2281 aggtgaacgg catcgccgag gcgcagagca tcatcgccga gaacatcgcc cggcgcggct
     2341 tcactacctt ctggacgcac ccggagcaac tggtcaacgc cgatgtccgc gatagctggc
     2401 gcgagacgat tgccgccgct gccgccgccc gcgacaaggg cgacctctgg atcgcgcccg
     2461 tgaccacgat cacgcactac tggcgcgatc tggcccaggt cagcgtgaac agcgccacga
     2521 acggcgacaa gctagcgatt accgtgtcca gcaaggccgc cgagccgctg gagggcgtga
     2581 cgctgaccct gccgcgcccg gcggcggagg tgcgcatcgg cggcgcggtc gtcaagcagc
     2641 cgcgccccac gcaggtcatc ctgccgcggc tcgacccgaa cgcgcccatc caggtcgaag
     2701 ttacgctcgc cgccgggggc agccgatgat ccaggccgca tcgatcacgc tggcgctcat
     2761 ggccgccgtc ggtggcctga tcctgggcgg cggattaagc gcggcgctac tgctggggct
     2821 gcggcggcgc ggcgcgaccc tgaacgggcg ccgcgccggg atcatcgccg ggctgggcgc
     2881 gctggtgctg gcggtgatcg cgggctgggg cgggctggtc ttcggcggcg acgcgcccat
     2941 ccccgccgcc tgccagggcg tgcaggcgca ggggcgcacc aatctcatcg ccaacccggc
     3001 cttcgcgccg gggccggacg gcaccctcgc cagttggacg atcactagca cgctggcgag
     3061 cggcgcgccc ggcgggtgg
//
LOCUS       DASUYC010000773         2419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_120485,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000773 DASUYC010000000
VERSION     DASUYC010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120485"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VF276_12680"
     CDS             <1..281
                     /locus_tag="VF276_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HEX6607757.1"
                     /translation="VTFHVFQLEDGATSEVRMPGTEGAGPLTAVVGREGGRIQVDVQG
                     ATQPWRVLLRGVETTGPVEGGSVQAEALGTLLIPEPGAGRVTVRLPKA"
     gene            337..1710
                     /locus_tag="VF276_12685"
     CDS             337..1710
                     /locus_tag="VF276_12685"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HEX6607758.1"
                     /translation="MTETTLMSPQQAGFPPDFIWGAATAAYQIEGAVREDGRGPSIWD
                     TFSHTPGKTKNGDTGDVADDHYHRWQEDIALMRELGLDAYRFSIAWPRIIPAGRGAVN
                     EAGLDFYDRLVDGLLAAGIQPFATLYHWDLPQALQDAGGWANRDTAPAFADYAEVVAR
                     RLGDRVRAWITLNEPWVVAFVGNLQGRHAPGFQDLPTALRVTHHLLLAHGLAVPRLRA
                     ATPSTPVGITLNLTPAEPASDSPEDRAAAARQDTYSNRLFLDPIRRGVYPAAAQAFLS
                     GATLPVQEGDLETIAAPLDFLGINYYARTVVRAAPGSGMLELEAVQPPGEYTTMGWEV
                     YPAGLRALLDRVHRDYAFPAYYITENGVAFVDVVETDGAVHDERRTAFLQGHFRAAAQ
                     AIRDGVPLRGYFIWSLLDNFEWGHGYSQRFGIVYVDYDTQRRIVKDSGRWYQQFLAAR
                     DTAPAPR"
     gene            complement(1811..>2419)
                     /locus_tag="VF276_12690"
     CDS             complement(1811..>2419)
                     /locus_tag="VF276_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017618494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6607759.1"
                     /translation="GSYIDYLTPGIILMTICTGSATTALSVRSDMSEGIIARFRTMAI
                     SRAAVLTGHVAGSMIRTVISIALLLAVAWLLGFRPSAGPVEWIAATGVLALFTFAIAW
                     LAVALGLVTGSPAAANASTMPLQFLLPLTSSAFVPPASMPEGLRWFAEYQPFTPITET
                     IRGLLLGTPIGDNGVLAVAWCAGLALVGYIWAKTSFNRGPVR"
BASE COUNT          387 a          856 c          842 g          334 t
ORIGIN      
        1 gcgtgacgtt ccacgtcttc caactcgaag acggtgccac gagcgaggtc cggatgccgg
       61 gcacggaggg cgccggcccg ctcactgccg tggtgggccg cgagggcggg cgcatccagg
      121 tggatgtgca gggggccacc caaccgtggc gcgtcctgct gcgcggcgtc gagacgaccg
      181 ggccggtgga aggcggcagc gtacaagccg aggcgctcgg cacgttattg atcccggaac
      241 cgggcgccgg tcgcgtgacc gtgcggctgc cgaaagcctg agcccgcgcg ccgggcgcaa
      301 agcggcgcag gccgcccata catgcgaggt agaactatga ccgagacaac cttgatgtcg
      361 ccccagcaag ccgggttccc gccggacttc atctggggcg ccgccaccgc ggcttatcag
      421 atcgagggcg ccgtgcgcga ggatggccgc ggccccagca tctgggatac gttcagccac
      481 acccccggca agaccaagaa cggggacacg ggcgatgtcg ccgacgatca ctaccatcgc
      541 tggcaggaag atatcgccct gatgcgtgag ctgggcctcg acgcctaccg cttctccatc
      601 gcctggccgc ggatcatccc ggcgggccgg ggcgcggtga acgaggccgg gctcgacttc
      661 tatgaccggc tggtggacgg cctgctggcc gccggcatcc agccgttcgc caccctctat
      721 cactgggatc tgccgcaggc gctccaggac gcgggcggct gggccaaccg cgacacggcc
      781 cccgccttcg ccgactacgc cgaggtggtg gcccgccggc tgggcgaccg ggtgcgcgcc
      841 tggatcacgc tcaacgagcc gtgggtggtc gccttcgtcg gcaacttgca gggccgccac
      901 gcgcccggct tccaggacct gccgacggcg ctgcgcgtca cccatcatct cctgctggcc
      961 cacggcctgg ccgtgccccg gctgcgcgcc gccaccccca gcacgccggt cggcatcacc
     1021 ctgaacctga cgcccgcgga accggcgagc gactcgccgg aggaccgggc cgccgccgcg
     1081 cggcaggata cctactcgaa ccgcctgttc ctcgacccca tccgccgtgg cgtctacccg
     1141 gcggcggcgc aagccttcct cagcggggcc acgctgccgg tgcaggaggg cgacctggag
     1201 acgatcgccg ccccgctcga cttcctgggc atcaactact atgcccgcac tgtcgtgcgc
     1261 gccgcgcccg gcagcggcat gctggaacta gaggcggtcc agccgcccgg cgagtacacg
     1321 acgatgggct gggaggtcta cccggcgggg ctgcgcgcgc tgctggaccg cgttcaccgc
     1381 gactacgcct tccccgccta ctacatcacc gagaacggcg tggccttcgt ggatgtggtg
     1441 gagacggacg gcgcggtcca cgacgagcgc cgcaccgcct tcttgcaggg ccacttccgc
     1501 gccgcggccc aggcgatccg ggatggtgtg ccactgcggg gttactttat ctggtcgctg
     1561 ctcgacaact tcgagtgggg ccacggctac agccagcgct tcggcatcgt ctatgtcgat
     1621 tacgacaccc agcggcggat cgtgaaggac agcgggcgct ggtatcagca gttcctggcg
     1681 gcgcgcgaca ccgcccccgc gccccggtaa gccggagcct ccaggcgcgg aaaagatcgc
     1741 ggcgcggcag agtggaaaga tccaaccacc cctgccgcgc cgccgatcag gcgttcgatc
     1801 ctgatcgggt ctagcggacc gggccgcggt tgaagctggt tttggcccag atatagccaa
     1861 ccagggcgag gcccgcgcac caggcgacgg ccagcacacc gttgtcgccg atgggggtgc
     1921 ccagcaagag gccgcggatg gtctcggtga tcggtgtgaa gggctggtac tcggcgaacc
     1981 agcgcaatcc ttcgggcatg gaggccgggg gcacgaacgc gctactggtg agcggcagca
     2041 ggaactgcag gggcatggtc gacgcatttg ccgccgcggg actccccgtc accagcccga
     2101 gcgccacggc cagccacgcg atagcgaagg tgaacaaggc cagcacgccc gttgccgcga
     2161 tccactcgac cggcccggcg ctgggccgga agcccagcag ccaggcgacc gcgagcagca
     2221 gcgcgatgct gatcaccgtg cggatcatgc tgccggcgac gtgtccggtg agcaccgccg
     2281 cgcgggagat cgccatcgtg cggaagcggg cgatgatacc ctcgctcatg tcactgcgga
     2341 cactcaaggc cgtcgtcgcc gacccggtgc agatggtcat caagatgatg cccggcgtga
     2401 gatagtcgat gtaactgcc
//
LOCUS       DASUYC010000774         3156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1753565, whole genome shotgun sequence.
ACCESSION   DASUYC010000774 DASUYC010000000
VERSION     DASUYC010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1753565"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VF276_12695"
     CDS             <1..233
                     /locus_tag="VF276_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607760.1"
                     /translation="LDADGATGTTPPDTFPLEEALSGGRYLYVLDSRLLLPVPGPATL
                     SGWRIGTDGSLTSVVDPAQIVLPFTAIGLAAD"
     gene            484..1599
                     /locus_tag="VF276_12700"
     CDS             484..1599
                     /locus_tag="VF276_12700"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HEX6607761.1"
                     /translation="MKKLLSIVTVAVAVLSLMVGLAPIVSRGADHLDGPLAEADGRMD
                     ITDVYAFESPSNHHNTVLIMNVDPAAGVLSPTSFRSTAAYEIRVIRNGHGDQPAMSND
                     SESNTLVYRVTFSAPNASGKQRLTLRCAPRGQCLDDKDLATDAPAAKDPSDNVADNPQ
                     DGASVILAQGWTDSTLSVRGGGRVRAGVFDDPFFFDLAAFRNNLTFCPGGVGTDFFLG
                     LNVASIVLEVPTSTLGRNLGIWGRTTLSGNQVDRMGRPAINTVFNNHNDPGKDLYNVG
                     RPRNDRRDYRAQFITVLEALGNDATRAGQLADFLLPDVLTYDTSAAEGFPNGRRLSDD
                     VIDIELGLLTNGAVTTDCVANDSTFRNSFPYLAPPNH"
     gene            1682..3004
                     /locus_tag="VF276_12705"
     CDS             1682..3004
                     /locus_tag="VF276_12705"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6607762.1"
                     /translation="MLPRRMVFLVGAVLLIIGASYVVNRGPTATPAASVAPAALDGED
                     VSGGVGMKTTDELIAFWDARVARDPRDFISMTFLAGSFMHKARETGDIAQYQRAETTI
                     HQALAIDPDYEGAQAYLAAIHYTEHDFTGALTLATQVYAAHPNIVSALATIGDAQLEL
                     GNYKEAEAAYQRLNQRNPGPAVESRLSRLAWLQGHPAQAIALMQQATAEAQDLALTGE
                     SAAWYQFQLGELYFQTGNPDRAAGYYSQALDLFPNYYLALAGLGKARAAQERYPEAIG
                     YYQQAVAIIPQPDFLAALGDLYTLTGQPAQAQQQYDTVEFIGKLAAINKVIYNRQLAL
                     FDANHDRKLDEALALTTQELTARKDIYGYDAQAWALYKNGRYQKAQAAMQQAMKLGTQ
                     DALLDYHAGMIAKSLGDTAQARTLLSRALARNPHFDLLQARIARAALP"
BASE COUNT          566 a         1136 c          956 g          498 t
ORIGIN      
        1 tgctggatgc cgacggcgcc accggcacca cgccgcccga taccttcccg ctggaggaag
       61 cgctgagcgg gggccgctat ctgtacgtgc tcgactcgcg gctgctgctg cccgtgcccg
      121 gtcccgccac ccttagcggc tggcgcatcg gcaccgacgg cagtctgacc tcggtggtgg
      181 acccggccca gatcgtccta cccttcaccg ccatcggcct ggccgcggat tagcgactgg
      241 ccccgtatcc ctgccttgcc gccccgcgcg gcagcggcgc cggcgcccgg cccaccgccc
      301 gctccggcgc cggcagggcg cgggctgccg cgcgaaccgg tatgagcaac atgggaaagg
      361 aggtgctgct ttgaattagt tccgggcgca ggcgcgcctc tgttccgggg gtcacagggc
      421 aatgcctggc tgcgtctcca agcggagcgc acaacacttt actacaggat agaggagcat
      481 ccaatgaaaa agctactctc gattgtgacc gttgccgtgg cggtgctgtc cttaatggtc
      541 ggcctggccc caatcgtctc tcgcggcgcc gatcacctgg acggtcccct ggcggaagcc
      601 gacgggcgga tggacatcac cgacgtttac gctttcgagt cgccgtccaa ccaccataac
      661 accgtcctga ttatgaacgt cgacccggcg gccggcgtgc ttagccccac cagcttccgc
      721 tcgacggccg cgtacgagat ccgcgtgatt cgcaacggcc acggcgacca gccggccatg
      781 agcaacgact ccgaatcgaa taccctggtc taccgggtca ccttctccgc cccgaacgcc
      841 agcggaaagc agcgcctgac tctgcgctgc gcgcccaggg gccagtgcct cgacgacaaa
      901 gatctcgcca ccgacgcgcc cgccgcgaaa gatccctcgg ataatgtggc ggataatccc
      961 caggatgggg cgagcgtgat cctggcccag ggctggaccg acagcaccct ttccgtccgc
     1021 ggcggcggcc gggtccgggc gggcgtgttc gacgatccct tcttctttga cctggcggca
     1081 ttccgtaaca acctcacgtt ctgccccggc ggcgtcggca ccgacttctt cctgggcctg
     1141 aacgtcgcct cgatcgtcct cgaagtgccg accagcaccc tgggccgcaa cctgggcatc
     1201 tgggggcgca ccaccctctc cggcaaccag gtggaccgta tgggtcgccc ggccatcaat
     1261 accgtcttca acaaccacaa cgatccgggc aaagacctct acaacgtcgg gcgcccgcgt
     1321 aacgaccgcc gcgactatcg cgcccagttc atcactgtgc tggaggcgct agggaacgac
     1381 gccacccgcg ccggccaact cgccgacttc ctgctgcccg acgtgctgac ctacgatacg
     1441 tccgccgcgg aaggcttccc gaatgggcgg cgcctgagcg atgacgtcat cgacatcgag
     1501 ctcggcctgc tcaccaacgg cgccgtgacg accgactgcg tcgccaacga ctccaccttc
     1561 cgcaacagct tcccgtatct ggctccgccg aaccactagg cgacgcggcg cctcgcggct
     1621 ggggaggggc ggggtttgaa catcccgcct ctccctcaaa actcggctct ggagggaaaa
     1681 gatgcttcct cgcagaatgg tttttctggt cggcgccgtg ctgcttatca tcggcgcgtc
     1741 gtatgtggtc aatcgcgggc ccaccgcgac cccggcggcg tccgttgcgc ccgccgccct
     1801 ggatggcgaa gacgtgagcg gcggggtggg catgaagacc accgacgagt tgatcgcttt
     1861 ctgggacgcg cgcgtggcgc gggacccgcg cgatttcatc agcatgacct ttctggccgg
     1921 aagcttcatg cacaaagccc gcgagaccgg cgacatcgcg cagtatcagc gggcggaaac
     1981 cacgatccac caggcgctgg cgattgatcc cgactacgag ggcgcgcaag cctacctggc
     2041 cgcgatccac tataccgaac acgacttcac gggggcgctg accctggcta cccaggtcta
     2101 cgccgcgcac cccaatatcg tctcggcgct ggcgaccatc ggcgacgccc agttggagtt
     2161 gggcaactac aaggaagccg aagccgccta ccagcgcctg aaccagcgca accccggccc
     2221 ggcggtcgag agccggctgt cgcgcctggc ctggctgcag ggccacccgg cccaggccat
     2281 tgccctgatg cagcaggcca ccgccgaagc ccaggacctg gcgctgaccg gcgaaagtgc
     2341 cgcctggtat cagttccaac tgggcgaact ctatttccag acgggcaacc ccgaccgggc
     2401 cgcgggctac tacagccagg cgctcgacct gtttcccaat tattacctgg ccctggccgg
     2461 gctgggcaag gcccgcgccg cgcaggaacg ctaccccgag gccatcggct actaccagca
     2521 ggctgtcgcc atcatcccgc agcccgattt cctggccgcc ctgggcgatc tctatacgct
     2581 gaccgggcaa ccggctcaag cccagcagca gtacgacacg gtcgagttca tcggcaagct
     2641 ggcggcgatc aacaaggtga tctacaaccg gcaactggcg ctgttcgacg ccaaccatga
     2701 tcgcaagctg gacgaggccc tggcgctgac gacccaggaa ctaacggcgc gcaaggatat
     2761 ttacggctac gatgcccagg cgtgggcgct ctacaagaac ggccggtatc aaaaagccca
     2821 ggcggccatg caacaggcca tgaaactggg cacgcaggat gcgctgctgg attaccacgc
     2881 cgggatgatc gccaagagcc tcggcgacac cgcccaggcc cggacgctgc tctcgcgcgc
     2941 cctggcccgc aatccgcact tcgacctcct gcaagctcgc atcgcccgcg cggcgctgcc
     3001 ctgagcggaa cgcagacttg ccacccaacc ccgcatcctg gactttttgc accggcacga
     3061 cgggctggaa taattctact atgcaccgcg aaggcgcgaa gcacacgaag accaggactg
     3121 atataaatcg tcttcgcgtt cttcgcgcct tcgcgg
//
LOCUS       DASUYC010000775         2424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1738597, whole genome shotgun sequence.
ACCESSION   DASUYC010000775 DASUYC010000000
VERSION     DASUYC010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1738597"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="VF276_12710"
     CDS             complement(<1..1074)
                     /locus_tag="VF276_12710"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX6607763.1"
                     /translation="MYSNARRVVVTGMGAVTPIGHSAAEYWRNLCAGVSGAGPITRFD
                     VSNYPVKIACEVKDFDPTKYMEARDVRRTGRFVHFALAATAQALADAEFSITEANAEM
                     VAVAFNTGGGGLVDMQNEIEVMEKKGMGRVSPFFIPSIIPNMAAGQVSLEFGAKGPVI
                     TSVAACASSVYSFIDGYHLIGRGEADVVIAGGTEAGLTPIAVAGLGNMQALSRNSTRP
                     PQEVSRPFDAKRDGFVFGEGAGAVILESLEHAQARGARIYAEVLGGAQTGDAYHITAP
                     APGGAGAALAMSRAIRYAGLEPADVDYVVAHGTSTPLNDVAETAAIKRAFGEHATKLA
                     ISSNKSMIGHLLGAAGVMSGIAAT"
     gene            complement(1089..2327)
                     /gene="fabF"
                     /locus_tag="VF276_12715"
     CDS             complement(1089..2327)
                     /gene="fabF"
                     /locus_tag="VF276_12715"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX6607764.1"
                     /translation="MRRVVVTGMGALTPVGNNLEETWTAFKEGKPGVARITLFDPSPY
                     EIQIGGEVKNFDNSSINPKELRHMDRSVQFAVVAAQEALRDAGYAITPENADRTGIII
                     GSAAGGLGVTLNQQKVLEERGPRRLSPFFLANMLPDSASGQVAIAVGARGPNMAVASA
                     CATGSHAIGEAWETIRRGDADAILAGGTDAVILPLVLAGFVVMRGLGNDPDPARACKP
                     FDKNRNGFVLSEGCGILLLENLEHAQARSARIYAEVIGYGSSNDAFDMFGQSESGGAA
                     IRMMRMALHKAEIAPEDVDYIVAHGTGTPLNDRVETLAIKQTFGDHARDLVVSSFKSM
                     TGHMMGASGAVGAINAVKAVQEGVIPPTTNYSTPDPDCDLDYNTGGGVREKDIDVAIS
                     NAIGLGGHNACVVVRKYQGA"
BASE COUNT          361 a          810 c          817 g          436 t
ORIGIN      
        1 cgtggcggcg atgcccgaca tgacacccgc ggcgccgagc aggtggccga tcatcgactt
       61 gttcgagctg atcgccagtt tagtggcgtg ctcgccgaag gcgcgcttga tggcggcggt
      121 ctcggccacg tcgttgagcg gcgtgctggt gccgtgggcc actacgtaat cgacatccgc
      181 cggctccagg cccgcgtagc ggatggcgcg ggacatcgcc agggcggcgc ctgcgccgcc
      241 cggcgccggg gcggtgatgt ggtaggcgtc gccggtctgc gcgccgccca gcacctcggc
      301 gtagatacgg gcgccacggg cctgggcgtg ctctagcgac tccaggatga cggcacccgc
      361 gccctcgccg aagacaaagc cgtcgcgctt ggcgtcgaag ggccggctga cctcctgcgg
      421 cgggcgggtg ctgttgcgcg aaagcgcctg catgttgccc agcccggcca cggcgatggg
      481 cgtgaggccc gcctcggtac ctccggcgat gaccacgtcg gcctcgccgc gcccgatcag
      541 gtggtagccg tcgatgaacg aatagacgct gctggcacag gcggccaccg aggtaatgac
      601 ggggcccttg gcgccgaact ccagcgagac ctgccccgcg gccatgttgg gaatgatcga
      661 ggggatgaag aacgggctga cgcggcccat gcctttcttc tccatgacct cgatctcgtt
      721 ctgcatatcg accagcccgc cgccgccggt gttgaaggcc acggcaacca tctcggcgtt
      781 ggcctcggtg atgctgaact cggcgtcggc cagggcctgg gccgtggcgg cgagcgcgaa
      841 gtggacgaag cggcccgtgc ggcgcacatc gcgggcttcc atgtacttgg tcgggtcgaa
      901 gtccttgact tcgcaggcga tcttgacagg gtaattgctc acgtcgaagc gggtgatcgg
      961 ccccgcgccg gagacccccg cacagaggtt gcgccagtac tcggcggcgc tatggccgat
     1021 gggcgtgacc gcgcccatcc cggtgacgac aacgcgccgt gcgttgctat acatgcaggt
     1081 ccctctcttt acgcgccctg gtacttgcgc accacgacgc acgcgttgtg gccgcccagc
     1141 ccaatcgcgt tgctgatcgc cacatcgatg tctttttcgc gcaccccgcc cccggtgttg
     1201 tagtcgaggt cgcagtccgg gtcgggtgtg gaatagttgg tggtgggcgg gatcacgccc
     1261 tcctggaccg ctttcacggc gttaatcgcg ccgacggcac ccgacgcgcc catcatgtga
     1321 ccggtcatcg acttgaacga cgagaccacc aggtcgcggg catggtcgcc gaaggtctgc
     1381 ttgatcgcca gcgtctcgac gcggtcgttg agcggcgtgc cggtgccgtg ggccacgata
     1441 taatcgacat cctccggtgc gatctcggcc ttgtgcaggg ccatgcgcat catgcgaata
     1501 gcggcgccgc cgctctccga ctggccgaac atatcgaagg cgtcgttgct tgacccatag
     1561 ccgatcacct cggcgtagat gcgggcactg cgggcctggg cgtgttccag gttttcgagc
     1621 agcaggatgc cgcagccctc ggagaggacg aagccgttgc ggttcttgtc gaacggcttg
     1681 caggcgcggg ccgggtcggg gtcgtttccc agcccgcgca tgacgacgaa gccggccagc
     1741 accaggggca gaatcacggc gtcggtgccg cccgcgagga tggcatcggc atcgccacgg
     1801 cggatcgtct cccaggcttc accgatggca tggctgccgg tggcgcaggc cgaggccacg
     1861 gccatgttcg gcccgcgcgc ccccacggcg atggccacct ggcccgacgc gctgtcgggc
     1921 aacatgttgg cgagaaagaa ggggctgagc cggcgcggcc cgcgctcttc cagcaccttc
     1981 tgctggttca gggtcacgcc cagcccgccc gccgccgagc cgatgatgat gcccgtccgg
     2041 tcggcattct ccggcgtaat cgcgtacccg gcgtcgcgca gggcttcctg cgcggccacc
     2101 acggcaaact gaacgctgcg gtccatatgc cgcagttcct tgggattgat gcttgagttg
     2161 tcgaagttct tgacttcgcc gccaatctgg atctcgtagg ggctgggatc gaacagcgtg
     2221 atccgcgcca cgccgggctt gccctccttg aacgcggtcc aggtctcttc gagattattg
     2281 cccaccgggg tgagcgcacc catccccgtc acgacgactc tgcgcatgct tctgcttcct
     2341 gttgctcact tcctgcttca ctacattcac tatccggtcg ggtgattact ccccatggct
     2401 aaagccaggg gcttctacgg attg
//
LOCUS       DASUYC010000776         3394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_815836,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000776 DASUYC010000000
VERSION     DASUYC010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815836"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            269..1141
                     /locus_tag="VF276_12720"
     CDS             269..1141
                     /locus_tag="VF276_12720"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEX6607765.1"
                     /translation="MVYSTLISAADLRPHLGEADWAVVDCRFALNEPERGRHDYLAAH
                     IPGAVYAHLTEDLSGPVVPGQTGRHPLPEVDRFAQVLAGWGIDARVQVVAYDASGGSM
                     AAARLWWMLHWLGHEAAAVLDGGWPAWQAAGYPEASGPEHRTPRTFTPHVHSELVLSS
                     AEVAARLHDPRTRLLDVRAAARYRGEQEPIDPVAGHIPGAASAPYEDTLDTAGRFRAP
                     EELRARFQGLLGAAPPEGAVAYCGSGVTAAHTLLALTHAGLGPGRLYAGSWSEWITDP
                     ARPIATGEEPQGGA"
     gene            1214..1504
                     /locus_tag="VF276_12725"
     CDS             1214..1504
                     /locus_tag="VF276_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX6607766.1"
                     /translation="MIFTLLEAHVAADQWQTLQHAYEAGLDRLPPQMVQTFLMQDARD
                     PTRWSILSFWHSREALDEYRQTAGTPEGILWFRAAGAEPTLAAGNVVAQAAQ"
     gene            1742..>3394
                     /locus_tag="VF276_12730"
     CDS             1742..>3394
                     /locus_tag="VF276_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015205464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX6607767.1"
                     /translation="MPVAQVDSDPIDAKQLLRVLMAVKKGDFSVRMPSDETGIAGKIA
                     DTLNDIIEMNERLTTELARVGTVVGHEGKITQRASLGDAGGGWAASVQAVNGLIGGLV
                     QPMTEVGRVIGAVARGDLSQTMVLEIDGEPLKGEYLRTANIVNTMVDQLGSFASEVTR
                     VAREVGTEGKLGGQAQVQGVAGTWKDLTDSVNLMAGNLTGQVRNIAEVTTAVANGDLS
                     KKITVDVKGEILELKNTINTMVDQLNAFASEVTRVAREVGTEGKLGGQATVRGVSGTW
                     KDLTDNVNLMAANLTGQVRNIAEVTTAVANGDLSKKITVDVKGEILELKNTINTMVDQ
                     LNAFASEVTRVAREVGPEGKLGGQAEVKDVAGIWKDLTDNVNYMAGNLTGQVRNIAEV
                     TTAVANGDLSKKITVDVKGEILELKNTINTMVDQLNAFASEVTRVAREVGTEGKLGGQ
                     AQVRGVSGTWKDLTDNVNLMAGNLTGQVRNIAEVTTAVANGDLSKKITVDVKGEILEL
                     KNTINTMVDQLRSFASEVTRVAREVGTDGKLGGQARGEGVSGP"
BASE COUNT          615 a         1109 c         1178 g          492 t
ORIGIN      
        1 agcccacagc cccgtgtcct cgatcagcag caggcgtccc gacccgtacg gcgacgcgcg
       61 cggcaccgcg cgctccagcc gcgggtttgc tttatcatag cgcccggcgc ggctaccgtc
      121 aactgctgcg ggcgtgcgcg gcggcgccag gccggccgtc tgcccccgtg cctgctcaac
      181 cgccagcctg tggtatactg cgctagacta ccttgcgccc gtcgtgacag tctgacggcc
      241 ggcgggggcc cttcgggagg acagcgcgat ggtttacagc acccttatca gcgccgccga
      301 tctgcggccc cacctgggcg aggccgattg ggcggtggtc gattgccgct ttgcgctcaa
      361 cgagcccgag cgcgggcggc acgattatct ggcggcccac atccccggcg ccgtctatgc
      421 ccacttgacc gaggatttat cggggccggt agtgccgggg cagacgggac ggcacccgtt
      481 gccggaggtg gatcggttcg cccaggttct ggcgggctgg ggcatcgacg cgcgggtgca
      541 ggtggtggcc tacgacgcca gcggcggatc gatggccgcg gcgcggctct ggtggatgct
      601 gcactggctc ggccacgagg cagcggcggt gctcgacggc ggctggcccg cctggcaggc
      661 ggcgggctac ccggaggcga gcgggccgga gcaccgcact ccgcgcacct tcacgccgca
      721 cgtccactcc gaactggtgc tcagcagcgc ggaggtggcg gcgcggctgc atgacccccg
      781 cacccgcctg cttgatgtcc gcgccgcggc gcgataccgg ggcgagcagg aaccgatcga
      841 cccggtggcc gggcatatcc ccggcgcggc cagcgcccct tacgaagata ccctggatac
      901 cgccgggcgc ttccgcgcgc cggaggagtt acgcgcccgg ttccagggcc tactgggcgc
      961 ggcaccgccg gagggcgccg tggcctattg cgggtcgggc gtcaccgccg cgcacaccct
     1021 gctggcgctg acccatgccg ggttggggcc gggccggctc tatgccggct cgtggagcga
     1081 gtggatcacc gacccggccc gccccatcgc caccggcgag gagccgcaag gcggcgcata
     1141 ggcgcgcccc ggcggcgctc gccggacgcg aatggatacc tcctgagttg cggaaagcga
     1201 gggatcaggc gcgatgattt tcacgctgct cgaagcccat gtggctgcgg accagtggca
     1261 gacattgcaa cacgcctatg aggcggggct ggaccggctc cctccgcaaa tggtccagac
     1321 ctttctgatg caggatgccc gcgaccccac ccggtggagc atcctgtcgt tctggcacag
     1381 ccgggaggcg ctcgacgaat accggcaaac cgccggcacc ccggagggca tcctctggtt
     1441 ccgggccgcc ggcgccgagc caaccctcgc tgccggcaac gtcgtggcgc aggccgcgca
     1501 gtagccgccc ccggctaggg ccggcggcac gacccaccta tacccgcggt cccgcgcggc
     1561 tgccatgcgg caaaggcggc atggtttgtg ctgcgaatcg cctatccccg ctgtcggttt
     1621 tcattgacat ttgggctact aatatgctaa gacatccgca ggtagcgccc gcgggaggtt
     1681 gggtgcccgc cgctgcctgc actttcgtcc taccttcaga cagcagctag aggagagtac
     1741 tatgccggtg gcacaggtgg actcggatcc catcgacgca aaacagcttt tgcgggtcct
     1801 gatggcggtc aagaaaggcg acttttcggt gcgcatgccg agcgacgaga cgggcatcgc
     1861 cgggaagatc gccgatacgc tgaacgacat catcgagatg aacgagcggc taaccaccga
     1921 actggcgcgg gtgggcaccg tggtgggcca cgagggcaag attacccagc gggcctcgct
     1981 cggcgacgcg ggcggcggct gggcggccag cgtgcaggcg gtcaacggcc tcatcggcgg
     2041 gctggtgcag cccatgaccg aggtgggccg ggtcatcggc gccgtggccc gcggcgatct
     2101 ctcgcagacc atggtcctgg agatcgacgg cgagccgctc aagggcgagt acctgcgcac
     2161 ggccaacata gtcaatacga tggtcgacca actcggctcg tttgcctccg aagtcacgcg
     2221 cgtggcccgc gaggtgggca cggaaggcaa actcggcggc caggcccagg tgcaaggggt
     2281 ggccgggacg tggaaagacc tgaccgatag cgtcaacctg atggctggga acctgaccgg
     2341 ccaggttcgc aacatcgccg aagtgaccac cgccgtcgcc aacggcgacc tctccaagaa
     2401 gatcaccgtg gatgtgaagg gcgagatcct cgaactcaag aacacgatca acaccatggt
     2461 cgaccaactg aacgccttcg cctcggaagt gacgcgcgtg gcccgcgaag tcggcaccga
     2521 aggcaaactg ggcggccagg cgaccgtgcg cggcgtgtcg ggcacctgga aggacctgac
     2581 cgacaacgtc aacctgatgg ccgccaacct gaccgggcaa gtccgcaata tcgccgaagt
     2641 gactaccgcc gtggcgaacg gcgacctgtc gaagaagatc acggtggacg tgaagggcga
     2701 gatcctcgaa ctcaagaaca ccatcaacac gatggtggat caactcaacg ccttcgcctc
     2761 cgaggtgacg cgcgtggccc gcgaggtggg ccccgagggc aagctgggcg ggcaggccga
     2821 agtgaaggac gtggccggga tctggaaaga tctcaccgat aatgtgaact acatggcggg
     2881 caacctgacc gggcaggtcc gcaatattgc ggaagtcacg acagcggtgg cgaacggcga
     2941 tttgtcgaag aagatcaccg tcgatgtgaa aggcgagatc ctcgaactca agaacaccat
     3001 caatacgatg gtggatcaac tcaacgcctt tgcgtcggaa gtgacgcgcg tggcccgcga
     3061 agtaggtacc gagggcaaac tcggcgggca ggcgcaggtg cgcggcgtgt cgggcacgtg
     3121 gaaggatctt accgacaacg tgaacctgat ggcgggcaac ctgaccgggc aggtgcgtaa
     3181 catcgccgaa gtcaccaccg ccgtcgccaa tggcgatttg tccaagaaga tcacggtgga
     3241 cgtgaaaggc gagatcctcg aactcaagaa caccatcaat acgatggtcg accagctccg
     3301 ctcattcgcg tctgaggtga cgcgcgtggc gcgcgaagtg ggcacggacg gaaaactcgg
     3361 cggccaggcc cgcggtgagg gcgtctccgg cccc
//
LOCUS       DASUYC010000777         2090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1597238, whole genome shotgun sequence.
ACCESSION   DASUYC010000777 DASUYC010000000
VERSION     DASUYC010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1597238"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1893
                     /locus_tag="VF276_12735"
     CDS             <1..1893
                     /locus_tag="VF276_12735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEX6607768.1"
                     /translation="APMTKAPTTGAGSLDPTFGTGGMVEVNAYEPGSDQARAVAILPD
                     GKILTGGYTQNGSASAVIRYLPNGAVDNTFGNGGLASATAPDGYDEDIIDSIVVQPDG
                     KIVTAGLAELDYTNPAYNYGFNATDTIVSRYNADGSLDLTFGNGGQTTLDLTTYYQDC
                     AGIKDEGRAVALDADGRIVVATSGSDQFGVARFNADGSLDGSFGNGGVSLVSFDGQSG
                     ASNDALVIQPDGKIVTGGYYSTNDPDAQYFALVRLNPDGSLDSSFGTDNMLATQVGNG
                     ARANLHALALQPDGKIVAGGGGGGGEFALIRANADGSLDTGFGDGGRVAEFISPAGPD
                     FITAVAVQANGKIVAVGQGSPNGGGDSDWAAARFNPDGSLDPTFGTGGKVITTFVPLW
                     EDVALAVAIQADGNIVAAGYARDPRVWSNMWWNTALVRYLGDAGAPPPPPPPPPPAGC
                     TVPFTDLAGNPFATYIENLYCRHIVDGYADNTFRPYSQATRGTLAKWVVLSRGWALDT
                     TGGPHFTDVPAGDPLYGYIETAYRHGVLSGYADHTFRPAANVTRGQMSKMIVNAMGWA
                     PVTPAAPHFRDVNAANPFYSQIETIYAHGLVSGYNCGAGCLEFRWGNDNTRGQLTKVL
                     SNAVSP"
BASE COUNT          363 a          737 c          706 g          284 t
ORIGIN      
        1 gctcccatga ccaaggctcc cacgaccggg gccggcagcc tggaccccac cttcggcacc
       61 ggcggcatgg tggaagtgaa cgcctacgaa ccggggtcgg accaggcgcg ggccgtggcg
      121 attctgcccg acggcaagat cctcaccggc ggctacacgc agaacggcag cgcctccgcg
      181 gtgatccgtt acctgcccaa cggcgccgta gacaacacct tcggcaacgg ggggctggca
      241 agcgccacgg cgccggacgg ctacgacgag gacatcatcg acagcatagt ggtgcagccg
      301 gacggcaaga tcgtcaccgc cgggctggcc gaactcgatt acaccaaccc ggcctataac
      361 tacggcttca acgccaccga caccatcgtc tcgcgctaca acgccgacgg cagcctcgac
      421 ctgaccttcg gcaacggcgg ccagacgacg ctcgacctga ccacctacta ccaggattgc
      481 gcgggcatca aggacgaggg ccgggcggtg gccctggacg cggacgggcg catcgtggtg
      541 gcgacctccg gcagcgacca gttcggcgtg gcccgcttca acgccgacgg cagcctggac
      601 ggcagcttcg gcaacggcgg cgtgtcgctg gtcagttttg acggccagag cggggcgagc
      661 aacgacgccc tggtgatcca gcccgacggt aagatcgtga ccggcggcta ctactcgacc
      721 aatgacccgg acgcgcagta cttcgcgctg gtgcgcctca acccggacgg cagcctggac
      781 agcagcttcg gcaccgacaa catgctcgcc acgcaggtcg gcaacggggc gcgggccaac
      841 ctgcacgcgc tggccttgca gcccgacggc aagatcgtgg ccgggggcgg cgggggtggc
      901 ggcgagttcg ccctgatccg cgccaacgcg gacggcagcc tggataccgg cttcggcgac
      961 ggcggcaggg tcgcggagtt catctcgccc gccggccccg acttcatcac cgcagtggcg
     1021 gtgcaagcca acggcaagat cgtggccgtg ggccagggtt cgcccaacgg cgggggcgac
     1081 tccgactggg cggcggcgcg cttcaacccg gacggcagcc tggacccgac cttcggcacc
     1141 ggcggcaagg tgatcaccac cttcgtgccg ctgtgggaag acgtggcgct ggccgtggca
     1201 atccaggcgg acggcaacat cgtggcggcg ggttatgcgc gcgacccgcg cgtgtggagc
     1261 aacatgtggt ggaacacggc gctggtgcgc tacctgggcg atgcgggtgc gcctccgcct
     1321 ccgcctccgc ccccgccgcc cgcgggctgc acggtcccgt tcaccgacct cgccggcaac
     1381 ccgttcgcga cctacattga gaacctgtac tgccgccaca tcgtcgacgg ctatgccgac
     1441 aacaccttcc ggccctacag ccaggcgacg cggggcacgc tcgccaaatg ggtggtgctg
     1501 agccggggct gggcgctcga caccaccggc ggcccgcact tcaccgacgt gcccgcgggc
     1561 gacccgcttt acggctacat cgagacggcc taccgccacg gcgtgctgag cggctacgcg
     1621 gaccacacgt tccgcccggc ggccaacgtg acccgcgggc agatgagcaa gatgatcgtc
     1681 aacgccatgg gctgggcgcc ggtcacgccc gcggccccgc acttccggga cgtgaacgcg
     1741 gccaacccgt tctacagcca gatcgagacg atctacgccc acgggctggt cagcggctac
     1801 aactgcggcg cgggctgcct ggagttccgc tggggcaacg acaacacccg cggccagttg
     1861 accaaggtgc tctcgaacgc ggtcagcccg taaggacgcg ctaacgtgta cagcgaaggg
     1921 gaacccccgg cgggttccct ttcgccatgc ccgatttcac cgcagagtgg ggtgtgtccc
     1981 ggtgggacac tggggggcat gaaagtcggc ttgtcactct gagcgcagcg aagagtctcg
     2041 tcttgtaccc caacgcgacg agattcttcg ctgcgctcag aatgacaagt
//
LOCUS       DASUYC010000778         2816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1276331, whole genome shotgun sequence.
ACCESSION   DASUYC010000778 DASUYC010000000
VERSION     DASUYC010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1276331"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            522..1586
                     /locus_tag="VF276_12740"
     CDS             522..1586
                     /locus_tag="VF276_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HEX6607769.1"
                     /translation="MTTWQEELRGSIRDAAGLRRVAGAAQAASDVEAVLAEYKMAIPP
                     YYLSLIDWNDPNDPIRLQAVAAPNELVTIPEERHDPIGDQRWSPVHRLTHRYPDRVLI
                     YPTYLCSMYCRFCFRKERLNEEEAGFPHDALAPALDYVASHPEIREVILTGGDPFVLA
                     DDKLAGLLRRIADIPHVEGIRIHTRVPVTLPMRVTPGLAAALDIPKMLWVVTHFNHPR
                     ELTPQAIAACHALHRAGARVLNQNVLLKGVNDSAEILAELYRGLVYRAGVKPYYLHHC
                     DMTLGVGRFRTTIEHGQAIMKQLRGHISGLCLPTYVLDIPGGAGKSPLGPNYIRGREG
                     DVWQIESYTGAIHEYRDTGT"
     gene            1583..2659
                     /gene="epmA"
                     /locus_tag="VF276_12745"
     CDS             1583..2659
                     /gene="epmA"
                     /locus_tag="VF276_12745"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HEX6607770.1"
                     /translation="MSTRPTWQRLREQPELLPRYLMRERVIRAIREFFYAQDFHEVET
                     PLLVPVPSMEPYLEVFETEFEDVRGGRQRAFLTNSPEYAMKRLLVAGLPRIFQICKAF
                     RNREDTGRKRNPEFTMLEWYRADADYTAIMADCEGLLAHLYAATRPASPAPGARLVLR
                     YQGMDVDLTPPWERISMREAFVRYAALDLDTVWDLPALRAEMERRGYTTGDSPWDELL
                     TQLFANEVEPHLGRTRPVILYDYPLAMAGLARRAQDPRYAERFEFYIAGLELGNAFSE
                     LNDAEEQAARLRADQAERRRLGKKQYAVDDDFLAALRAGMPPAGGIAVGVDRLIMLFA
                     DVASIHDTLWMPGRELFGPATLED"
BASE COUNT          493 a          951 c          903 g          469 t
ORIGIN      
        1 cggcggcctc aaagagatcg tgcagttcat agccgtggcg cgattgctgc gacagcaggg
       61 ccagcagcgc atgttgcacg gggccacgct cgattttgct ccgggtcagg cttgaagtca
      121 taaataccta cttgctatct agatttcaga taaaatatac acctggtatc tagtttcgtc
      181 aataggactt ttggctgatc ttcaacatcc aggccgcctg cgccctcgtc gggcgggaca
      241 cgccgccaag cttgggcgcg cctgatggtt tggcatcccg gtgagcaacc aacctaaccc
      301 ccctgccccc ttcccgcggc gggaaggggg gaatcgcgaa cgcgagggaa agtgactccc
      361 ctccccgtgg cggggagggg cgggggagag gttgcaaggc ccgccgagtt cgcccggttg
      421 ggccaatttg ccggtctatg atataatagc tggtgcaaaa aacaagacta ctcaacatac
      481 tcagcactca gcactcagca ctcagcactc aggactacgg tatgacgacc tggcaggaag
      541 aattacgggg cagcattcgc gacgcggcgg gcctgcgccg cgtggccggg gcggcccagg
      601 ccgcgtccga cgtggaagcc gtgctggccg agtacaagat ggcgatcccg ccctactacc
      661 tgagcctgat cgactggaac gacccgaacg atccgatccg cctgcaagcg gtggccgcgc
      721 cgaacgaact ggtcaccatc ccggaggagc gccacgaccc catcggcgac cagcgctggt
      781 cgccggtgca ccggctcacg caccgctacc cggatcgcgt gctgatctac cccacgtacc
      841 tctgctcgat gtactgccgc ttctgcttcc gcaaggagcg gctgaacgag gaggaggcgg
      901 gcttcccgca cgacgcgctg gccccggccc tggactacgt cgcgagccac ccggagatcc
      961 gcgaggtgat cctgaccggc ggcgacccgt tcgtgctggc cgacgacaag ctggccgggc
     1021 tgctgcggcg catcgcggac atcccgcacg tcgagggcat ccgcatccac acccgcgtgc
     1081 cggtcacgct gcccatgcgc gtgacgccgg gcctggcggc ggcgctcgac atcccgaaga
     1141 tgctctgggt ggtcacgcat ttcaaccacc cgcgcgaact gacgccgcag gccatcgccg
     1201 cctgccacgc gctgcaccgg gcgggggcac gcgtgctcaa ccagaacgtg ctgctcaagg
     1261 gcgtcaacga cagcgccgag atcctggccg aactctaccg gggcctggtc taccgggcgg
     1321 gcgtgaagcc gtattacctg caccactgcg atatgacgct gggcgtgggc cgcttccgca
     1381 cgaccatcga gcacggccag gcgatcatga agcagttgcg cgggcacatc tcgggcctct
     1441 gtctgcccac ttacgtgctc gacatccccg gcggcgcggg caaatcgccg ctcggtccca
     1501 actatatccg gggccgtgag ggcgatgtct ggcagatcga gtcctacacg ggcgccatcc
     1561 acgaatatcg agataccggc acgtgagcac ccgccccacc tggcagcgcc tgcgcgagca
     1621 accggagttg ctgccccgct acctgatgcg cgagcgcgtc atccgcgcca tccgtgagtt
     1681 cttctacgcg caggacttcc acgaggtgga gacgccgctg ctggtgcccg tgccgagcat
     1741 ggagccgtac ctggaggtct tcgagaccga gttcgaggat gtgcgcggcg ggcggcagcg
     1801 cgccttcctg accaactcac ccgaatatgc catgaagcgg ctgctggtgg ccgggctgcc
     1861 gcgcatcttc cagatctgca aggccttccg caaccgcgag gacacgggac gcaagcgcaa
     1921 ccccgagttc accatgctgg aatggtaccg cgccgatgct gactacacgg cgatcatggc
     1981 cgactgcgag gggctgctgg cccatctcta tgccgccacc cggcccgcgt ccccggcgcc
     2041 cggtgcgcgt ttagtgctgc gctatcaggg catggatgtt gatctaacgc ccccctggga
     2101 gcgcatcagt atgcgtgaag cgttcgtccg ctatgccgcg ctcgacctgg acaccgtctg
     2161 ggatttgccc gcgttgcgcg ccgagatgga gcggcgcggc tatacgaccg gcgactcgcc
     2221 gtgggacgaa ctgctcacgc agttgttcgc caacgaagtc gagccgcatc tgggccgcac
     2281 ccgaccggtg atcctctacg actacccgct ggcgatggcc gggctggccc ggcgcgcgca
     2341 ggacccgcgc tatgccgagc ggttcgagtt ttacatcgcc gggctggagc tgggcaacgc
     2401 cttctccgag ttgaacgacg cggaggagca ggccgcccgc ctgcgcgccg accaggccga
     2461 gcggcgccgc ctgggcaaga aacagtacgc tgtcgacgat gatttcctgg ccgccctgcg
     2521 cgctgggatg ccgcccgcgg gcggtatcgc cgtgggggtg gaccgcctga ttatgttgtt
     2581 cgcggacgtg gcctcaatcc acgatacact atggatgccg gggcgcgagt tgtttggccc
     2641 ggctactttg gaggactaac actatggcac gtattcccgc cgatcagttt cgccgcgggc
     2701 tgaccgtcgt ctttaacgac cagccgcacc agatgacgga tgtcgagttc gtcaaccccg
     2761 gcaaaggctc gtcgttctac cgcacgacgc tcaagaacct caagaccggg cgcgtg
//
LOCUS       DASUYC010000779         2820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_584613,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000779 DASUYC010000000
VERSION     DASUYC010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584613"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..1448
                     /locus_tag="VF276_12750"
     CDS             42..1448
                     /locus_tag="VF276_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX6607771.1"
                     /translation="MKLHAHKGQWLVVSLLMIMSLLLAACGETAATNTPVPPAAAATD
                     TPAAAAPTNTTAPAPAATDTTAPAATATTASGGSSTGTGGDLTVGLLTDDSGSLAIYG
                     PMLERGFELGLNYATKGTNQVAGKTIKVVTKDTASNVDTGTALAREAIEKDGAKVLVG
                     VPSSGVALAVSGLAAQNKLIYISTPAATPDLTGKNFNPYTFRAGRTSVQDALTMGAAL
                     VGLGKKFVQIAPDYAFGRGSAAAFYAVIKGAGGTFTVNDNDKDAGTVFAPQDTTDFTP
                     YINQVLDAKADVLVVTWAGAGFVPLFQQMQQLGVFKNMVVATGMGDNQTMAKGYADAV
                     GSVGVSVYHYALFDNPVNNWLVEQHKAKYNSPPDLFTESGFNAAIMLVKALEATNGDP
                     AADGMIKALEGMQFDGPKGSYTVRPEDHVLLQPMTLVKLKNTTDPDFKFFDLVKLFKP
                     EETAPPCAVPAEMNRCKK"
     gene            1610..2389
                     /locus_tag="VF276_12755"
     CDS             1610..2389
                     /locus_tag="VF276_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6607772.1"
                     /translation="MTDIVIETRDLSCYFGGLRAVDGVNLQVQAGTLHSIIGPNGAGK
                     TTLFNLISGRIKPTRGQVFLRGRDITGVPLHRMAHLGVGRSFQITNLFPNLTVLENAR
                     LAAQALGHDSLKLWAPARRFRRYEEQAHAALRAVGLDGKAALAAAVLPHGDKRKLELA
                     VLLAADPQVLLLDEPTAGMASEQVPMLLDIIHAVRARTGKTILLVEHNMGIVMNVSDR
                     ISVMHQGHLLAEGTPAEIRANPAVQSAYLGESYNDAEPAHR"
     gene            2367..>2820
                     /locus_tag="VF276_12760"
     CDS             2367..>2820
                     /locus_tag="VF276_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6607773.1"
                     /translation="MPNLLTVESIQTYIGQFHILEGVSLTVPQGSITALLGRNGAGKT
                     TTLRSIMGLLAPREGRIVLDGQDIAGRPAYNIAALGVGYVPEHRAIFRDLTVEENLKI
                     AERRKGDLARHADLIFNLFPDLKRFYSLPGGQLSGGQQQMLAIARALVP"
BASE COUNT          518 a          987 c          904 g          411 t
ORIGIN      
        1 cggggcgctg agttatcgta ccgtattcag aggaggagag catgaaacta cacgcgcaca
       61 agggtcaatg gctcgtggtg agcctgctga tgatcatgag cctgctgctg gccgcctgcg
      121 gtgagacggc ggccaccaac acgcccgtgc cgcccgcggc ggcggcgacc gataccccgg
      181 cggcggcggc cccgaccaac acgacggcgc ccgccccggc ggccaccgac acgaccgccc
      241 cggcggccac ggccaccacg gcgagcggcg gcagcagcac gggcaccggc ggcgacctca
      301 ccgtcggcct gctgaccgac gactccggct cgctggcgat ctacggcccg atgctggagc
      361 gcggctttga actgggcctg aactacgcga ccaagggcac caaccaggtg gcgggcaaga
      421 cgatcaaggt cgtgaccaag gacaccgcga gcaacgtcga tacgggcacg gcgctggcgc
      481 gcgaggccat cgagaaggac ggcgccaagg tgctcgtcgg cgtgcccagt tcgggcgtgg
      541 ccctggccgt ctccgggctg gccgcgcaga ataagctgat ctacatctcc actccggcgg
      601 ccacccccga cctgaccggt aagaacttca acccctacac cttccgcgcg ggccgcacca
      661 gcgtgcagga cgcgctgacc atgggcgccg ccctggtcgg cctgggcaag aaattcgtgc
      721 agatcgcccc tgactatgcc ttcgggcgcg gctcggctgc cgccttctac gcggtgatca
      781 agggcgcggg cggcaccttc accgtcaacg acaatgacaa ggacgcgggc accgtcttcg
      841 cgccgcagga cacgaccgac ttcacgccct acatcaacca ggtgctcgac gccaaggccg
      901 acgtgctggt cgtgacctgg gccggcgcgg gcttcgtgcc gctgttccag cagatgcagc
      961 aactgggcgt cttcaagaac atggtcgtcg ccacgggcat gggcgacaac cagaccatgg
     1021 ccaagggcta cgccgacgcc gtcggatcgg tgggcgtgag cgtctatcac tacgcgctgt
     1081 tcgacaaccc agtcaacaac tggctggtcg agcagcacaa ggccaagtac aacagcccgc
     1141 ccgacctgtt caccgaaagc ggcttcaacg cggcgatcat gctggtcaag gcgctggaag
     1201 ccactaacgg cgacccggcg gcggacggca tgattaaggc gctggaaggc atgcagttcg
     1261 acggccccaa ggggtcctac accgtgcggc ccgaagacca cgtcctgctc cagccgatga
     1321 cgctggtcaa gctgaagaac acgaccgacc cggacttcaa gttcttcgac ctggtcaagc
     1381 tcttcaagcc ggaggagacc gcgcccccgt gcgccgttcc cgccgagatg aaccgctgca
     1441 agaaataagc tatcagccgt cagccgttag ccgtcagcgt ttgctgatgg ctgatggctg
     1501 gcagctagcg gctaatccca gatcggcgcg aagcatatta ctgatcactg accactgacc
     1561 actgaccact cgtacctgac ggctgattgc tgacggctga cggctaccta tgactgacat
     1621 tgtgatcgaa acgcgcgacc tatcgtgcta ttttggcggc ctgcgggccg tggacggggt
     1681 gaacctgcaa gtgcaggcgg gcaccctcca ctcgattatc gggccgaacg gtgcgggcaa
     1741 gaccacgctc ttcaacctga tcagcgggcg gatcaagccg acgcgcggcc aggtcttcct
     1801 gcgcgggcgc gacatcaccg gcgtgccgct gcaccgcatg gcccacctgg gcgtgggccg
     1861 ctcgttccag atcaccaacc tgttcccgaa cctgaccgtg ctcgaaaacg cgcggctggc
     1921 cgcccaggcg ctgggccacg atagcctcaa gctctgggcg cccgcccgcc gcttccgccg
     1981 ctacgaggag caagctcacg cggccctgcg ggcggtgggc ctcgacggca aggccgcgtt
     2041 ggcggcggcg gtgctgccgc acggcgataa gcgcaaactc gaactcgccg tgctgctggc
     2101 cgccgacccg caggtgctgt tgctggatga acccaccgcc gggatggcgt ccgaacaggt
     2161 gcccatgctg ctcgacatta tccacgccgt gcgcgcccgc accggcaaga cgatcctgct
     2221 ggtcgagcac aacatgggca tcgtgatgaa cgtctccgac cgcatctcgg tgatgcacca
     2281 ggggcacttg ctggccgagg gcacacccgc cgagatccgc gccaacccgg cggtgcaaag
     2341 cgcctatcta ggggaaagct acaacgatgc cgaacctgct caccgttgag tcgatccaga
     2401 cctacatcgg ccagttccat atcctggaag gcgtgtcgct gacggtgccg cagggcagca
     2461 tcaccgccct gctgggccgc aacggcgcgg gcaagacgac gactctgcgc tcgatcatgg
     2521 gcctgctggc cccgcgcgag gggcgcatcg tgctcgacgg gcaggacatc gccgggcgcc
     2581 cggcctacaa catcgccgcg ctgggcgtgg gctatgtgcc cgaacaccgg gccatcttcc
     2641 gcgacctgac cgtggaggag aacctgaaga tcgccgagcg gcgcaagggc gacctggccc
     2701 gccacgccga tctcatcttc aacctcttcc ccgacctcaa gcgcttctac agcctgcccg
     2761 gcgggcaact gtcgggcggg cagcagcaga tgctcgccat cgcccgcgcc ctggtgcccg
//
LOCUS       DASUYC010000780         5223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1840138, whole genome shotgun sequence.
ACCESSION   DASUYC010000780 DASUYC010000000
VERSION     DASUYC010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5223
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1840138"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..2326)
                     /locus_tag="VF276_12765"
     CDS             complement(176..2326)
                     /locus_tag="VF276_12765"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX6607774.1"
                     /translation="MHLPGPAVPDAFDELPCAALVLDVQQGTIVLGNSAARALLAEIP
                     LEAEDRSLRALAAAVLAPATRPARVRGSARRAATRRTAAGLLTLAVPDPSGGGRTVEV
                     LLSPLDETAFGAGFALLLIRPPGAPSWGTEWLREAHDALACRAPEFHTILARATASPR
                     VAAPAANGAGPQPVPVLALEAAAHAMVLTDREGMITWANPAFSRMTGYAVAEVLESPL
                     RLYQGGQQNGLSYQERWTAIQDGAVWHGELINRRKDGSLYIEEETISPVRDATGAITH
                     FLSVGEDITERKQHEERLRHQASHDLLTNLPNRQTLEEALERAAQHARRGRVSSLLFL
                     DLDNFKIVNDTLGHAAGDQLLIILTQILSEQLRQGDLLARVGGDEFAVLLEGLAVEPA
                     AAIAERMRAGIAEFPFTLQGHRFDLGLSIGLVPIDGQQPADVLLAEGDIAMYTAKEQG
                     RNRIIRFQPDAENLVRLTEAHQWARRIKDALRDDRMLFYFQPVLRVGDATVVDYEALL
                     RMRAEDGTLIQPGGFLPAAERFGLMPLLDRWMVQEAVRTLAAHPAIRLTVNLSAYSPA
                     DEALLDHIGEQLRDQAVAPARLAFAMSEAAVLQDLRRAEHWIRRLTDLGCRFVLDEFG
                     TGFASLTYLRHLPVDQVKIDGSFIRMLVPDPSTREIVRAIHTLARALGKETVAIFVED
                     AATLDIVRAIGVTYAQGYHLSYPTPDLPPVPAAS"
     gene            complement(2452..2793)
                     /locus_tag="VF276_12770"
     CDS             complement(2452..2793)
                     /locus_tag="VF276_12770"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX6607775.1"
                     /translation="MTGTPEIWVERRDKPRVQIAYPATVRGIDANGEAFEVNTLIDNL
                     GAGGLFVHLPRPVAQGVQLFAIVRLATALGDAVPAPRIATRGVVVRTEPGQDGECGVA
                     LAFTQHRFLYD"
     gene            complement(2890..3885)
                     /locus_tag="VF276_12775"
     CDS             complement(2890..3885)
                     /locus_tag="VF276_12775"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX6607776.1"
                     /translation="MNPFTQCPRCGHVLPQPAPACPVCGCLLGPLPPVEGKLLRNGDF
                     RVIRRLDRGGMASLYLAEATSTGGLRAVKELRSPPNRKARATAEAMFHEEARLLARLS
                     REHPAIPRYYDSFMDSGSFYLVLEFVPGQNLQQYLADRGGILPVPEVIDYLRQVVDVL
                     ATIHGLHPDPIIHGDIKPSNLVRRPDGQVALVDFGLARATVRMPAYVPSRSSAFGSPG
                     YTPMEQWEGHPGPASDIFALGATMHHLITGRNPRAAFTNLAKVSLAELSTLTNFPPIT
                     TLVPDVPPMLDTIIMQMVRRRAAERPTARDLKARLSRLDAVLSPQAFGDNVETRE"
     gene            4301..4759
                     /locus_tag="VF276_12780"
     CDS             4301..4759
                     /locus_tag="VF276_12780"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX6607777.1"
                     /translation="MSKQRTRAARRRTAPRGVRRGSALLELALVLPFLAALLLGVADI
                     ARAYGEQVAIAEAAHQGARAWVLTGGNDATAVQAAIDNAINDPDLSGRITRLNICRDP
                     SCAPDYTPGAVTVRITYRYNLLFGFFDDIVGGNQLVFSNSATMPLGVTGP"
     gene            4783..>5223
                     /locus_tag="VF276_12785"
     CDS             4783..>5223
                     /locus_tag="VF276_12785"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX6607778.1"
                     /translation="MLRATLFPLPTRHGRRGQAMVEFALTATLLLLLVFAIIEVSRAI
                     LIINTLSNGVREAAHFAALHPEQDAATLTANAHSRVADAGLGLADPATLQVQVDCPSC
                     RGGPAECIDPALLAPPAAGPACDALAQRLTVTATVAYTFTTVAPI"
BASE COUNT          813 a         1760 c         1846 g          804 t
ORIGIN      
        1 taggggccgg ttgcaaaccg gccggtagcc gcccaggaca cagggatgcc tgtgtagggg
       61 ccggttgcaa accggcctgc cgcccaatcc ggtcagccac gggccggttt gcaaccggcc
      121 cctacagcgc aaacgcagac tccacgctgc gtttggcggc tcaggggcgc ggaactcagg
      181 acgccgcggg cacggggggc aaatcgggcg tggggtagct gaggtggtag ccctgggcat
      241 aggtgacgcc gatggcgcgc acgatgtcca gcgtggcggc gtcttctacg aagatggcga
      301 ctgtttcctt gcccagggcg cgggccaggg tgtggatcgc ccgcacgatc tcccgcgtgc
      361 tggggtcggg caccagcatg cggatgaacg agccgtcgat cttgacctgg tccaccggca
      421 gatggcgcag gtaggtcagc gaggcaaagc cggtgccgaa ctcgtcgagc acgaagcggc
      481 agcccaggtc ggtcaaccgg cggatccagt gctcggcgcg gcgcagatcc tggagaacgg
      541 cggcctcgct catggcgaag gccaggcgcg cgggcgccac ggcctgatcc cgcaattgct
      601 cgccaatgtg gtcgagcaac gcctcgtccg ccgggctgta ggcggacagg ttcacggtca
      661 ggcgaatggc ggggtgcgcg gccagggtgc gcaccgcttc ctgcaccatc cagcggtcca
      721 gcagcggcat cagcccgaag cgctcggcgg cgggcagaaa tccgcccggt tggatcagcg
      781 tgccgtcctc ggcccgcatc cgcagcagcg cctcataatc gaccaccgtg gcgtcgccca
      841 cgcgcagcac cggctggaag tagaagagca tgcggtcgtc acgcagggcg tccttgatgc
      901 ggcgcgccca ctggtgggcc tcggtcagcc ggacgaggtt ttcggcgtcg ggctggaagc
      961 ggatgatgcg gttgcgcccc tgttccttgg ccgtatacat ggcgatgtca ccctcggcca
     1021 gcagcacgtc cgctggttgc tgcccatcga tcggcaccag gccgatgctc aggcccaggt
     1081 cgaagcggtg cccctggaga gtgaagggga actcggcgat cccggcgcgc atccgctcgg
     1141 cgatggccgc ggcgggttcc accgccagcc cttccagcag caccgcgaac tcgtcgccgc
     1201 ccacccgcgc cagcaggtcg ccctggcgca actgctcgga caggatctgc gtcaggatga
     1261 tcagcaactg gtcgcccgcc gcgtggccca gggtgtcgtt gacgatcttg aagttgtcca
     1321 ggtcgaggaa cagcaggctg ctcacccggc cccggcgcgc gtgctgcgcc gcccgctcca
     1381 gggcttcttc cagggtctgc cggttgggca gattggtcag caggtcgtgg ctggcctggt
     1441 ggcgcaggcg ctcctcgtgc tgtttgcgct cggtaatatc ctcgcccaca ctgagaaagt
     1501 gcgtgatcgc gccggtcgcg tcgcgcaccg ggctgatcgt ttcttcctcg atatagaggc
     1561 tgccgtcttt gcgccggttg atcagttcgc cgtgccagac ggcgccatcc tggatggccg
     1621 tccagcgctc ctggtaggac aggccgttct gctggccgcc ctggtagagc cgcagcgggc
     1681 tctccagcac ctcggccacg gcatagcccg tcatccggct gaaggccggg ttggcccagg
     1741 taatcatgcc ctcgcggtcg gtgagcacca tggcgtgggc ggcggcttcc agcgccagga
     1801 ccgggacggg ctgcggtccg gcaccgttgg cggccggggc ggcgacccgc ggggaagcgg
     1861 tcgcgcgggc gaggatggtg tggaactccg gcgcccggca ggccagcgca tcgtgcgcct
     1921 cgcgcagcca ctcggtgccc caggacggcg cgcccggcgg gcggatgagc agcagcgcga
     1981 accccgcgcc gaaggcggtc tcgtccagcg ggctgagcaa cacctcgacc gtgcgcccgc
     2041 cgccgctggg atccggcacg gccagggtca gcaggccggc ggcggtgcgg cgggtggccg
     2101 cgcggcgagc cgagccgcgg acgcgcgccg ggcgggtcgc cggggccagg accgccgccg
     2161 ccagcgcccg caggctgcga tcctccgctt ccagggggat ctccgcgagc agcgcgcgcg
     2221 cggcgctatt gcccagcacg atggtgcctt gctggacatc gagaaccagg gctgcgcagg
     2281 gaagctcgtc gaaggcatcc ggtacagcgg gaccgggtag gtgcatggag tgagcctcat
     2341 cccgcccccg actcgatccc gccggggcca ggccgggcgg aaggagcagg cgcggaagcc
     2401 gtgcgatgct actaaggcgc cggggggcca ggcttgacgg cggccaccgg gctagtcgta
     2461 taagaagcgg tgctgggtga aggccagggc cacgccacat tcgccgtcct ggcccggttc
     2521 ggtgcgcacg acgaccccgc gagtggcgat acgcggcgcg ggcacggcat cgcccagcgc
     2581 cgtggcgagg cgcacgatgg cgaaaagctg gaccccctgg gccaccggcc gcggcagatg
     2641 aacaaagagt cccccggccc ccagattgtc gatcagcgta ttgacctcga atgcctcgcc
     2701 gttggcgtcg atgccgcgca cggtcgccgg gtaggcgatc tgcacgcgcg gcttgtcgcg
     2761 gcgctcgacc cagatctcgg gagtccccgt catcccgcta cctcccgcca acatggaggc
     2821 tggtgcccga taggccgcct cccggccggt ttccccaaac cgccccccgc gccggggcgc
     2881 ggtcatatgc tattctcgcg tttcgacatt gtcgccaaac gcctgcggcg acagcacggc
     2941 atcgagccgg ctcagccggg ccttcaaatc gcgggccgtg ggccgttcgg cggcccgccg
     3001 gcgcaccatc tgcatgataa tcgtgtccag catcggcggc acatccggca ccagcgtcgt
     3061 gatcggcggg aaattggtca gcgtgctcaa ctcggccaga ctgaccttcg ccaggttggt
     3121 gaaggcggcg cgcgggttgc gcccggtgat caggtggtgc atggtcgcgc ccagggcaaa
     3181 aatatccgac gcgggaccgg gatggccctc ccactgttcc atgggcgtgt agccggggct
     3241 gccgaaagcc gacgagcgcg agggcacata ggccggcatg cgcacggtgg cacgcgccag
     3301 cccgaagtcg accagggcga cctgcccatc ggggcggcgc accaggttcg agggcttgat
     3361 atcgccgtgg atgatcgggt cgggatgcag gccgtggatc gtcgccagca cgtcgaccac
     3421 ctggcgcaga taatcgatca cctcgggcac ggggaggatg ccgccgcgat ccgccagata
     3481 ttgctgcaga ttctgaccgg gcacaaattc cagaaccagg tagaacgagc cgctgtccat
     3541 aaacgagtca tagtagcgcg ggatcgccgg atgttcgcgg ctgagccggg ccagcaggcg
     3601 cgcttcttcg tggaacatcg cttcggcggt ggcgcgcgct ttgcggttgg gcgggctgcg
     3661 cagttccttg acggcgcgca ggccgccggt cgaggtggct tcggccaggt acaggctcgc
     3721 catgccgccg cggtcaaggc gccggatcac gcggaagtcg ccgttgcgca gcagcttacc
     3781 ctcgacgggc ggcagcggcc ccagcaggca gccgcagacc ggacacgcgg gcgccggttg
     3841 cggcagcacg tgcccgcaac gagggcactg tgtgaaaggg ttcataggct ctggatatcc
     3901 tgcttcccgc cggacaatgc agcctccatg ccgcgcggga gaacggtatt tatgattcgc
     3961 tggttcgggc gtatccgggc aaattatctc gtacttcagt atatgcgcgg ctgctgttac
     4021 tgtcaacacg caccggcagg cgcaacgtgt ggcgacgccg gagcgcaagg atcaggcgga
     4081 tggcgacgcg gcgtaatttt tcgccgtgca cagccattat aatagtagaa ccccgccggt
     4141 acaaggggcg ggcgaatcgc gcctgggcgc cctcggcccc cggtgcgccg ccggttgacc
     4201 ccatatcgta taatcaggta tgatgatcag gcagcctcga ttgggcaatg gcacaccggg
     4261 gccggcctgg ggcgcgacgg caggccggcg agagagccgt atgagcaagc agcgaacaag
     4321 ggccgccagg cgacgaacag caccgcgcgg ggtccggcgg ggtagcgccc tgctggaact
     4381 ggccctggtg ctgccctttc ttgccgcgtt gctcctaggc gtcgccgaca ttgcgcgcgc
     4441 ctacggcgag caggtggcga tcgcggaagc cgcgcaccag ggcgcgcgcg cctgggtact
     4501 caccggcggc aacgacgcca cagcggtcca ggcggccatc gacaacgcga ttaacgatcc
     4561 cgatctcagc gggcgcatca cacgcttgaa tatttgccgc gatcccagtt gtgcgcctga
     4621 ctatacgccc ggtgccgtga ccgtgcgcat tacctatcgc tataatctgc tgttcggctt
     4681 cttcgacgat atcgttggcg gcaatcagtt ggtgttctcg aacagcgcga cgatgcctct
     4741 gggtgtcacc ggcccctagc ccctgcggcc ccaaggaccc ttatgctgcg tgctactctg
     4801 tttcccctgc cgacccgcca cggacgccgc ggccaggcga tggtcgagtt tgcgctgacc
     4861 gccacgctgc tcctcctgct ggtgttcgcc attatcgagg tgtcgcgggc catcctgatc
     4921 atcaacacgc tcagcaacgg ggtgcgcgaa gccgcgcatt ttgccgcctt gcatccggag
     4981 caggacgcgg ccacgctgac cgcgaacgcg cacagccggg tggcggatgc cggcctgggc
     5041 ctggccgatc ccgccaccct ccaggtgcag gtcgattgcc caagctgtcg cggcggcccg
     5101 gctgagtgca tcgacccagc cttgctggcg ccaccggcgg cgggcccggc ctgcgatgcg
     5161 ctggcgcagc ggctgacggt gacggccacc gtggcctaca ccttcaccac ggtcgcgccg
     5221 atc
//
LOCUS       DASUYC010000781         2170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1370794, whole genome shotgun sequence.
ACCESSION   DASUYC010000781 DASUYC010000000
VERSION     DASUYC010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370794"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..2031
                     /locus_tag="VF276_12790"
     CDS             184..2031
                     /locus_tag="VF276_12790"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX6607779.1"
                     /translation="MKTMQWPVRESLYGWRVRLRLWTHAVGVDQLDAALRGLAVGIVL
                     LLSLLGYNYAEARPSVLPFSIPPAGIAAALVLYNVAVVVLLGVPWRHRPGFMLFALDW
                     LVVSLAVVLTGGFYSPFLVLYYALVIGGALRLDFQPTLVLVGACSLVYAALSRDTPPP
                     GVSPAFHLPLLVVGVTSLLMVAMTALTMRRVVDVENARVHLEQQTAQRFALLNDLTRT
                     VLSASPDSAALLRTIAAIAPNALQADCALAVLLDRQGRPGRVAVDQGNAPDLTPGALA
                     LALTATAQRAPLVLENLPADPRGGDIRACLGRVEAVVCAPLLADTEAVGALIVGSYSP
                     RHWTPDDVSLLGAIGRQIALSVRLAQLYDLEREKAARSAERERVERDLLNTVSHELRT
                     PLTAIKTGVSGLLAGSAARPPAEARLLQNIDRSTERLILLINDVLDMARLRAGRVTLA
                     REQLDLGEVIQDALATVRPLAEAKNQTLVTDLPARAAQCACGLQAPADRRRVEQVLLN
                     ILNNATKYTPPGGRITVGAAAEPGQVRVWVHDTGPGIDPADQRRIFERFYIGPREGGR
                     PDATGLGLAIANSLIELHGGQIGVQSSPGTGSLFYFTLPTAAPLVPADV"
BASE COUNT          295 a          784 c          754 g          337 t
ORIGIN      
        1 cgaagggcgc gatccagatc agcagacggg tggcatagcg caacagcagt ttgagatcat
       61 ccatcaagcg actatagcac agccgtcgcg cggttgtcaa cgcgggcgcc cggcgcttat
      121 aatggtatag cggtactggt acgtaattag atcagcattg gatcagcagg ttggtagcgg
      181 cgtatgaaga cgatgcaatg gccggtcaga gaatcgctat acggctggcg ggtgcggctc
      241 cggctctgga cccatgccgt cggcgtcgat cagctcgacg cggcgctgcg cgggctggcc
      301 gtcggcatcg tgctgctgct cagcctgctg ggctataact acgccgaagc gcgcccgtcg
      361 gtcctgccgt tctccatccc gcccgccggg atagccgccg cgctggtgct ctacaatgtg
      421 gccgtggtcg tgttgctcgg cgtgccctgg cgccaccggc ccggcttcat gctcttcgcg
      481 ctcgactggc tcgtggtcag cctggcggtc gtgctgacgg gcgggttcta cagccccttc
      541 ctggtgctct actacgcgct ggtgatcggc ggcgcgctgc ggctcgattt ccagcccacg
      601 ctggtgctgg tcggcgcctg ctcgctggtc tacgccgcct tgagccggga cacgccgccg
      661 ccgggcgtca gtcccgcctt ccacctgccc ctgctcgtcg tcggcgtgac ctcgctgctg
      721 atggtggcga tgacggcgct gaccatgcgc cgcgtggtcg acgtggagaa cgcgcgggtc
      781 cacctggagc agcagacggc gcagcggttc gccctgctga acgacctgac gcgcaccgtg
      841 ctgagcgcct cgcccgactc ggcggcgctg ctgcgcacca tcgcggccat cgcgcccaac
      901 gccttgcagg ccgattgcgc gctggccgtg ctgctcgacc ggcagggccg gcctgggcgc
      961 gtcgcggtgg accagggcaa cgcgcccgac ctgacgccgg gcgccctggc cctggcgctg
     1021 accgcgaccg cgcagcgcgc gccgctggtg ctcgaaaatc tgcccgccga cccgcgtggg
     1081 ggcgatatcc gcgcctgcct ggggcgggtg gaagccgtgg tctgtgcacc cttgctggct
     1141 gataccgaag ctgtaggcgc gctgatcgtc ggcagctaca gcccgcgcca ctggaccccc
     1201 gacgacgtga gcctgctggg ggcgattggc cggcagatcg cgctctccgt gcgtctggcc
     1261 cagctctacg acctggagcg cgagaaggcg gcccgcagcg ccgagcgcga gcgcgtggag
     1321 cgcgacctgc tcaacacggt ctcgcacgag ctccgcaccc cgctgacggc gatcaagaca
     1381 ggggtgagcg gcctgctcgc gggcagcgcc gcgcggccgc ccgccgaggc gcgcctgcta
     1441 caaaacatcg accgcagcac ggagcgcctg atcttgctca tcaacgacgt gctcgacatg
     1501 gcccgcctgc gcgccgggcg cgtgaccctg gcccgtgagc agctcgacct cggcgaggta
     1561 atccaggacg ccctggcgac ggtgcgcccg ctcgccgagg ccaaaaatca gacgctggtg
     1621 acggacctgc cggcccgcgc cgcgcagtgt gcttgcggat tgcaggcgcc cgccgaccgc
     1681 cgccgcgtgg agcaggtgct gttgaacatc ctgaacaacg cgaccaaata cacgccgccc
     1741 ggcgggcgca tcaccgtggg cgcggcggcg gagccggggc aggtgcgcgt ctgggtgcat
     1801 gataccggcc ccggcatcga ccccgccgac cagcggcgca tctttgagcg gttctacatc
     1861 ggcccgcgcg agggtgggcg acccgacgcg accggcctcg gcctggcaat cgccaactcg
     1921 ctcatcgaac tccacggcgg gcagatcggc gtgcaaagca gccccggcac gggcagcctg
     1981 ttctacttca cgctgcccac cgccgcgccc ctggtgcccg ccgatgtctg atcccggcgc
     2041 cgtggcgatc ctcgtctcgt ccctgctgtt ccgcccgcgc cacctgtcgc ccgcccgcgc
     2101 cgaggatgtc taccgggcgg gggagaccct ggcgctgggc ctcgacatcg ccctggccgc
     2161 ggcgggggtc
//
LOCUS       DASUYC010000782         3551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_354012,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000782 DASUYC010000000
VERSION     DASUYC010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354012"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..797)
                     /locus_tag="VF276_12795"
     CDS             complement(24..797)
                     /locus_tag="VF276_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018620976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX6607780.1"
                     /translation="MKANSLRAKLQRGEVAYGMLTPIYDPAIVEVIGHLGFDFYMMDG
                     EHGAGGPWHLEHLVRACETVGMTPLVRVPTSEPKAVLQYLDTGVMGVMLPGVREATDV
                     QRLVDAVKYPPVGRRGVAGVRANDYLLGPMAQADYVRFANEQTLILPQIETLEAVDNI
                     EALVRVQGIDGFIVGPRDLSMAMGFYDGPRHPEVRAVIDHVFEVVLGAGLWIGTTAPT
                     GEEAADLVARGARLILGSVAWLLQVGAGRFFQAVHQAEP"
     gene            complement(802..1095)
                     /locus_tag="VF276_12800"
     CDS             complement(802..1095)
                     /locus_tag="VF276_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HEX6607781.1"
                     /translation="MIVLVAKYHVKPGHGDTVEAALRRMAPLVLAQEPGCTQYRVHRS
                     TENPDLFLLYEEYVDQAAIEAHRRMPQFQEIIEGTIVPLLDKREREFYNVVAG"
     gene            1341..1655
                     /locus_tag="VF276_12805"
     CDS             1341..1655
                     /locus_tag="VF276_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607782.1"
                     /translation="MKPGNQLQGVAWYLGTIQYGTLLFGGGALWFLAAWLTGFFGGDL
                     GAQALACLLLLALITFEAWWSHMPTRGNPAGDYLVDFLSSAAIAGFGMAVVLAILLWL
                     PR"
     gene            complement(1687..2013)
                     /locus_tag="VF276_12810"
     CDS             complement(1687..2013)
                     /locus_tag="VF276_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607783.1"
                     /translation="MNNQWVLVGGGQFGGNIEVHRQSAILVCPGPCYRPDEGTIYLTP
                     RNVQEVRIANVTGTLVTVVPRDPTINDSFVFDLATAQWVAPPGASPYPVYATPPQQTP
                     TAVPPP"
     gene            complement(2463..2789)
                     /locus_tag="VF276_12815"
     CDS             complement(2463..2789)
                     /locus_tag="VF276_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607784.1"
                     /translation="MNNQWVLVGGGQFGGNIEVHRQSAILVCPGPCYRPDEGTIYLTP
                     RNVQEVRIANVTGTLVTVVPRDPTINDSFVFDLATAQWVAPPGASPYPVYATPPRQTP
                     TAAPPP"
BASE COUNT          587 a         1161 c         1154 g          649 t
ORIGIN      
        1 cctgggtggg tctcaggtaa gatctacggt tccgcctggt gcacggcctg gaagaaccgc
       61 cctgcgccca cctgcaacag ccaggccacc gagccgagga tcaggcgagc gccccgcgcg
      121 accaggtcgg cggcttcctc gccggtcggc gcggtggtgc cgatccagag gcccgcgccc
      181 agcaccacct caaacacgtg gtcgatgacg gcgcgcactt cgggatgccg cgggccgtcg
      241 tagaagccca tcgccatcga gaggtcgcgc gggccgacga taaagccgtc gatgccctgg
      301 acgcgcacca gcgcctcgat attgtcgacg gcctccaggg tttcgatctg cggcaggatc
      361 agcgtctgct cgttggcgaa gcgcacataa tcggcctgcg ccatggggcc gagcaggtag
      421 tcgttggcgc gcaccccggc cacgccgcgc cggcccaccg gcggatactt gacggcgtcc
      481 accaggcgct gtacgtcggt cgcctcgcgc acgcccggca gcatcacacc catcacgccg
      541 gtgtcgaggt attgcagcac ggccttaggc tcgctggtcg gcacgcgcac cagcggggtc
      601 atgcccacgg tttcgcaggc gcgcaccagg tgctccaggt gccatgggcc gcccgcgccg
      661 tgctcgccgt ccatcatata gaagtcgaag cccaggtgcc cgatcacctc cacgatggcc
      721 gggtcgtaga tcggcgtgag catgccgtag gcgacctcgc cccgctggag tttcgcgcgc
      781 agtgagtttg ctttcatggc gctacccggc caccacgttg taaaattcgc gctcgcgctt
      841 gtccagcagt ggcacgatgg tgccctcgat gatctcctgg aactgcggca tgcggcgatg
      901 ggcctcaatc gccgcctgat cgacatattc ctcatagagc aggaacaagt ccgggttttc
      961 ggtcgagcga tgcacccggt actgggtgca gcccggctcc tgggccagca ccagcggcgc
     1021 catgcggcgc agcgcggcct cgaccgtatc gccgtggccc ggcttgacat gatatttcgc
     1081 gaccagcaca atcatcggcg ccttcctcct gcatagcgac gtgcgtagcc gggacgcggc
     1141 tacccccgcc cttgtcgtgt cctggcccta ttgtagcagg tttgccgggg cgccgcgcgg
     1201 gttgggcgcg cttgcgccgg gcggtataat ggatacaacc gcgcgaatgc ggcgcgatcc
     1261 gcgcccgtgt ccgcccggcg cgtgcgccct atgctgtgac aaacccaccg gcggccctgc
     1321 ggatcgcgtg gaggcgcggc atgaagcccg gtaaccagtt gcaaggggtg gcctggtatc
     1381 tcggcaccat ccagtacggc acgctgctgt tcggcggcgg cgcgctctgg ttcctggccg
     1441 cctggctgac cggcttcttc gggggcgacc tcggcgcgca ggcgctcgcc tgcctgctgc
     1501 tgctcgcact catcaccttc gaggcgtggt ggtcgcacat gcccacgcgc ggcaaccccg
     1561 ccggcgacta cctcgtggac ttcctcagca gcgccgccat cgccgggttc ggcatggccg
     1621 tggtcctggc gattctgctc tggctgccac gctgacccgc ccccctgtgc gctgttaccc
     1681 accacctcac ggcggaggca ccgctgtggg cgtctgctgc ggaggcgtgg cgtataccgg
     1741 gtagggcgac gctcccggtg gcgcgaccca ctgcgccgtt gccagatcga acacaaacga
     1801 gtcgttgatg gtcggatcgc gcggcactac cgtcaccaag gtgcctgtca cgttggcgat
     1861 ccgtacttcc tgcacattgc gcggggtcag ataaatggtg ccttcatcgg ggcggtagca
     1921 cgggccgggg cagactaaga tcgcgctctg ccgatgcacc tcaatgttgc cgccgaactg
     1981 cccgccgccc acgagcaccc attgattgtt cacccgcgct tcccagcagc ttggaaacat
     2041 cggcatacta gaatggagcg gcggaataca gtcggtatcg gcaatcaatc ctacccccag
     2101 tgtcgccgta gggccggtag tttcggtagg atacggggta gtggtgggcg tgagcgcggt
     2161 ctcggtaagc atatgattgt agcgctgaac aatttgcgta gctcccgccg cgcgggtcgg
     2221 atcaatgagg gaccctggcg ggtagccggc tttgaactct tcaaacacgg cagtggggtc
     2281 gcgggtcggt gtaccatcag gcagttgtgt taccgctgtc gggaccaggg tggcacccgg
     2341 cagggtggcc gtttcgctag gccccaccgt gtcgaccggt gtagtggccg gcgaggctgt
     2401 cataccacac gccactagca caatgccgga taacgctagc cagagcacta gcccccaccg
     2461 cctcatggtg gcggcgccgc tgtgggcgtc tggcgcgggg gcgtggcgta taccgggtag
     2521 ggcgacgccc ccggcggcgc gacccactgc gccgttgcca gatcgaacac aaacgagtca
     2581 ttgatggtcg gatcgcgcgg cactaccgtc accaaggtgc ccgtcacgtt ggcgatccgt
     2641 acttcctgca cattgcgcgg ggtcagataa atggtgcctt catcggggcg gtagcacggc
     2701 cccggacaga ctaagatcgc gctctgccga tgcacctcaa tgttgccgcc gaactgcccg
     2761 ccgcccacga gcacccattg attgttcacg cgcgcttccc agcaactggg aaacatcggc
     2821 atactagaat ggagggggtc aatacaaccc gtgtcggcga gtaggcctac gccgggtgtg
     2881 gtggtcggag cagccgtcaa ggtaggcggc ggagtagtgg tgggcgtgag cgcggtctcg
     2941 gtgagcatac gattgtactg ttgcacagcc acagtcgccg ctgcggcgtt tgtgggatca
     3001 atgagcgccc ctggcggata gccgcctttg tgctcctcga acaccgctgt agggttacgg
     3061 gtcggtgtac catcaggcag ttgtgttacc gctgtcggga ccagggtggc acccggcagg
     3121 gtggccgttt cgctaggccc caccgtgttg accggtgtag tggccggcga ggctgtcata
     3181 ccacaggcta ttccagcaac acctactaca gtcactacta acataacaag cattctcacc
     3241 ttacggctga tcatcttcat ttccccgccg cattttaatg tgatcccgta tattgcccta
     3301 ataacgaagt atttttacag caccatgact atagcatagt tagcgcggtt atgccacgcg
     3361 aaatgcacgg catcccggcc atcatggacg tactggccgc gctggaaacg ccctggcggt
     3421 ggcgccagtg gccgcgtccg gttgacgccg ggtccccaaa catactaaga tgcttacgca
     3481 gggtccgcgc cgggcgcgtt acttggggca gggcgccggt tgcaggccgc gctggccgaa
     3541 tatgcggacg t
//
LOCUS       DASUYC010000783         2434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1799760, whole genome shotgun sequence.
ACCESSION   DASUYC010000783 DASUYC010000000
VERSION     DASUYC010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1799760"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..402)
                     /locus_tag="VF276_12820"
     CDS             complement(166..402)
                     /locus_tag="VF276_12820"
                     /inference="COORDINATES: protein motif:HMM:NF017879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HEX6607785.1"
                     /translation="MPVLDIRVGDVYRLRKPHPCGSFDWQVRRTGADIRMVCVGCGHA
                     VMLPRSTFERRVKTLLSRDESPAPASTPTDDKDC"
     gene            771..2153
                     /locus_tag="VF276_12825"
     CDS             771..2153
                     /locus_tag="VF276_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015187396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-6-methoxyphenol hydroxylase-like
                     oxidoreductase"
                     /protein_id="HEX6607786.1"
                     /translation="MDTSHRDHAIVIGGSMAGLLTARALTDLFARVTVIERDRLPDGP
                     HPRKGVPQARHVHILLLRGQAILEQWFPGLEDELGALGAPRLDVTQDGLWLNRAGWGP
                     VFPSGRYGRTCSRDLREWAVRRRLRAIPAVRFREECDVVGLLAGPTGACVTGVRVRPR
                     GQTPGGAAVEEDLAADLVVDAGGRGSRAPEWLAALGYAKPQELVINSFLGYSSRYYAP
                     PENFAPPWQGLYIQPAPPAHMRGGGIYRVEDGRWLVTLVGIGGDYPPTADPDFLEFAR
                     SLRSPVLYDALRHATPLSPVAGYRGTANRWRYYERLARRPDQFVVIGDAACAFNPVYG
                     QGMTAAAQGAATLAAALRDQGRRHPDGDLRGLAARFQRQLARSNRPLWAIATLEDFRC
                     PQTVGGRPTPGDRLAQRYIDRVLRRSTTDRVAYLTLVEVLQLVQPLSALVRPGMLARV
                     LRPGGAPLPV"
     gene            complement(2155..>2434)
                     /locus_tag="VF276_12830"
     CDS             complement(2155..>2434)
                     /locus_tag="VF276_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607787.1"
                     /translation="LAAPGVAEVLFAFEPRWSAWRIIVQHQAVAVGLILIAVARTWGE
                     FNPANPVTWLYVGGMAGFWVAMIILLYVMDSRRPKTAARPRPAPTGIG"
BASE COUNT          375 a          835 c          826 g          398 t
ORIGIN      
        1 ggtcacctgc accaccaggg cgaagttgat catctgctgg gcgagttgcg aaaagatctg
       61 ggcgatccag agatacagga agttgtgatt acgcagcacg gcgcggaacg ccgcgctctg
      121 gttagcgttg gtgtttgcga cgacgctggt ggcttctgcc atacgttagc aatccttatc
      181 gtccgttgga gtggatgccg gcgcggggga ttcgtcacga gacagcagtg tcttgacccg
      241 gcgctcgaaa gtgctgcgcg gtaacatcac ggcgtggccg cagcccacac acaccatacg
      301 gatgtcggcg cctgtgcgtc gcacttgcca atcgaagctg ccgcagggat gcggtttgcg
      361 caggcgatac acatctccca cccggatatc caaaacgggc atgggatagc tctctttcta
      421 gcttggcggc gcagcgggtc cgggcggacc cgattccagc ccatattata ccacaactat
      481 tcgcccggcg aacgcaattt gcctggtctc agaatagtac aaaatccagg accggctggt
      541 acaatagtac cggggcacgg cgctccccgg cgcggcggcg cccggcgcgt agcccggtta
      601 taaattgcgc ccgcccgcgc tcggttactc tgcgcggcgc gggccgctct atccggctta
      661 gaacgcccca gcctgttggc cgatcgacag gcacttgaca ggccgagagc gcagggttag
      721 gcttagcgct ggatcgactc aggttctcgg caccacacag gaacacgccc atggatacct
      781 cgcatcggga ccatgcgatc gtcatcggcg gcagcatggc cggactcttg acggcgcggg
      841 cgctcaccga cctgttcgcc cgcgtcaccg tgatcgagcg cgaccgcttg cccgacggcc
      901 cgcacccgcg caaaggcgtg ccgcaggccc gccacgtgca tattctgctg ttgcgagggc
      961 aggccatcct ggaacaatgg tttccgggcc tggaagatga gctgggcgcg ctcggtgcgc
     1021 cgcgcctcga cgtgacccaa gacggcctct ggctcaaccg cgcgggctgg gggcccgtgt
     1081 ttccgtccgg ccggtacggg cggacctgta gccgcgatct gcgcgaatgg gcggtgcggc
     1141 gccggttgcg cgccatccct gccgtgcgct tccgcgagga gtgcgatgtg gtgggcctgc
     1201 tggccggccc aaccggcgcg tgtgtgaccg gggtccgggt ccgtccgcgt ggccagaccc
     1261 ccggcggtgc agccgtggag gaagacctgg ccgccgatct ggtcgtggat gccggcgggc
     1321 gtggatcgcg cgcgccggag tggctggcag ccctcggcta cgccaagccg caggagctgg
     1381 ttatcaactc tttcctgggt tacagcagtc gctactacgc cccgccggag aacttcgcgc
     1441 ccccgtggca gggcctgtat atccagcccg cgccgccggc ccacatgcgc ggcgggggca
     1501 tctatcgcgt cgaggacggg cgctggctgg tgaccctggt ggggattggc ggcgattacc
     1561 cgcccaccgc cgaccccgat ttcctggaat tcgcccgcag cctgcgcagc ccggttctct
     1621 acgacgccct gcggcacgcc acgccgctct cgcccgtcgc cggctaccgc ggcacggcca
     1681 accgctggcg gtattacgag cgcctggcgc gccggcccga ccagttcgtc gtgattggcg
     1741 acgccgcctg cgccttcaat ccggtctatg ggcaggggat gacggccgcc gcccagggcg
     1801 ccgcgacact ggccgccgcc ctgcgtgacc aaggccgccg ccatccggac ggcgatctcc
     1861 gcggcctggc ggcgcggttc cagcggcaac tcgcccgcag taaccggccg ctctgggcca
     1921 ttgccaccct ggaagatttc cgctgtccgc agacggtagg gggccggccc acgccgggcg
     1981 accggctggc ccagcgctat atcgaccggg tgttgcgccg gtccacgacg gatcgcgtgg
     2041 cctatctgac attggtcgag gtgttacaac tggtccagcc gctcagcgcc ctggtcaggc
     2101 cgggtatgct ggcgcgggtc ctgcggcccg gcggcgcccc actcccggtg tgatctagcc
     2161 tatgcctgtc ggcgccgggc ggggccgggc cgcagttttg ggccggcgcg agtccatcac
     2221 atagagcagg atgatcatgg cgacccagaa accggccatc ccgccgacgt atagccaggt
     2281 caccgggttg gccggattga actcgcccca cgtgcgcgcg accgcgatca ggatcagccc
     2341 gacagcgaca gcctggtgtt gcacgataat gcgccaggca ctccagcgcg gctcgaaggc
     2401 aaacaggacc tcggcgacgc cgggcgcggc cagc
//
LOCUS       DASUYC010000784         2627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1276791, whole genome shotgun sequence.
ACCESSION   DASUYC010000784 DASUYC010000000
VERSION     DASUYC010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1276791"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VF276_12835"
     CDS             complement(<1..193)
                     /locus_tag="VF276_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607788.1"
                     /translation="MELGDAQPTTPGRAAARPLVVPRLRAVPWRLVAFGTLGAATMLN
                     RYTLTIAGLHAKLEHLAILT"
     gene            complement(249..1856)
                     /locus_tag="VF276_12840"
     CDS             complement(249..1856)
                     /locus_tag="VF276_12840"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEX6607789.1"
                     /translation="MRTLAAMAGLLAAGAALFAYLLIRFPYDGLYGQDAYAYYYQALA
                     LWREIRGLPSAPGALFDAHALRWPLGYHLQLILGYLLSGDAAGGRALTLLLAWLAPAL
                     LYLIIREILRDAPPGRQVIAGLIGGGALLLTGTYARMGLSLMADVPALFWSLLAVYCA
                     LRAWPPPARDESRAVASHPGWAAATGVALGLAVLVRYGSVLLGAALLVYLLLRAYPPG
                     GPRTLRSLPWRGLAAAGLGFAVTLLPELAYALTHPGGLGYGIWLSDWRLDNLFSRTLT
                     SADGVATFPQPPIAFYLLSPLTDAPAGFLSILALPAAILGLGVLLRDRRRAVAGLLLT
                     WWLVPALFFSGSPYEAHRFVLAYLPALTTLAGIGGAVAVIWVGAAVRRPRVARTMAGG
                     VLAGLVLLGMAGGALQTARSVRDWVAFHAAAKTEERGVIALARAAAGPGGTVAPRVVC
                     FGATAALYHYTGWPVLELYNADTPEIAEFLAAPGPRLVVLPEASMATQWAGTPLAARW
                     DWLRTHYTLTRQGTFGSYTVYTLTGGP"
     gene            complement(1971..>2627)
                     /locus_tag="VF276_12845"
     CDS             complement(1971..>2627)
                     /locus_tag="VF276_12845"
                     /inference="COORDINATES: protein motif:HMM:NF037411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607790.1"
                     /translation="PPDCPVHLDPDLALDSVPRLPGWKPLFGQAGAAERHLQNQGVGA
                     GDIFIFYGWFRNVVEQAGRYRYVPEDPGRHVIFGWLQIEQRIPVDDRARIPVWARDHA
                     HCCRAQRDKLDSLYISTDRIVLPNATVDKPGAGIFPCFHPDLCLTAPGSPRSIWQLPA
                     WMYPGAGRSALSYHRNLQRWAAGLDHVLLQTVGRGQEFVLDCDEYPEALDWLAALLRI
                     "
BASE COUNT          459 a          942 c          908 g          318 t
ORIGIN      
        1 gcgttaggat cgccaggtgc tccaacttcg catgcaaccc ggcgatggtc agggtatagc
       61 ggttgagcat cgtggccgcg cccagcgtgc cgaaggccac caggcgccag ggcacggccc
      121 gcagccgggg caccaccagc gggcgggccg cggccctacc gggtgtcgtc ggctgtgcgt
      181 cgcccagttc catccctcgc gttcctcccg tgcctagtcc gcgcgattct cgccccgcgg
      241 tttgcccatc agggaccgcc ggtcagcgtg tagacggtat agctgccgaa tgtgccctgg
      301 cgcgtcagcg tgtaatgggt gcgcagccaa tcccagcggg cggccagcgg cgtgcccgcc
      361 cactgcgttg ccatgctcgc ctccggcaag accaccaggc gcggtcccgg cgccgccagg
      421 aattccgcga tctccggcgt atcggcattg tacagctcca gcaccggcca gcctgtgtag
      481 tggtacagcg cggcggtagc cccaaagcag accacgcgcg gcgccaccgt cccgccgggg
      541 cctgccgccg cccgcgccag ggcgatcacc ccccgctcct cggttttagc ggcggcgtgg
      601 aacgccaccc agtcgcgcac ggatcgcgcg gtttgcaacg ccccgcccgc catgcccagc
      661 agcaccaggc ccgccagcac cccgccggcc atcgtgcgcg ccacccgcgg gcgtcggacc
      721 gcggccccca cccagatcac cgcgacggcg ccgccgatcc cggccagcgt ggtcagggcc
      781 ggcagatagg ccagtacgaa gcggtgggct tcgtaggggc tgccgctaaa gaacagggcg
      841 ggcaccagcc accaggtgag gagcaagccg gccacggccc ggcgccgatc ccgcagcagc
      901 acacccaggc ccaggatcgc tgccggcagc gcgaggatcg ataggaaccc cgccggggca
      961 tcggtcagcg ggctgagcag atagaaggcg atgggcggct gcgggaaagt cgccacgccg
     1021 tcggcgctgg tcagcgtgcg gctgaacagg ttgtccagcc gccagtccga cagccagatg
     1081 ccgtagccga ggccgccggg gtgggtcagc gcgtaggcca gttcgggcag cagggtcacg
     1141 gcaaagccta accccgccgc cgccagcccg cgccacggca gggaccgcaa ggtgcgcgga
     1201 ccgccgggtg ggtacgcccg cagcagcaga tacaccagca aggccgcgcc cagcagcacc
     1261 gaaccatagc gcaccagcac ggcgagcccg agcgccacac cggtcgccgc cgcccagccg
     1321 gggtgcgacg ccacggcgcg cgactcgtcg cgggcgggcg gcggccaggc gcgcaatgcg
     1381 cagtagacgg ccagcaggct ccagaacagg gccggcacat cggccatcag cgagaggccc
     1441 atgcgcgcat acgtgcccgt cagcagcagc gcgcccccgc cgatgagccc ggcgatgact
     1501 tgccgaccgg ggggcgcgtc gcgcaggatc tcgcggataa tcaggtagag cagcgccggg
     1561 gcgagccagg ccagcagcag cgtgagcgct cgcccgcccg cggcatcccc gctcagcagg
     1621 tagccgagga tgagctgcag gtggtagccg agcggccagc gcaaggcgtg cgcgtcgaac
     1681 agcgcgcccg gcgcggacgg cagcccgcgg atctcccgcc agagcgccag cgcctggtaa
     1741 tagtacgcat aggcgtcctg cccatacagg ccgtcgtagg ggaagcggat cagcaggtag
     1801 gcgaacagcg ccgcgccggc ggcgagcagc cccgccatgg cagccagggt gcgcatggcg
     1861 ggatgatacg ccagttgcgc ctgccggtca agccgccggc accgccccac gccggtgcgc
     1921 gataccggaa ccggctgccg gcaggtgggg agagggcttc aagttacggg ctatatgcgc
     1981 agaagggcgg ccaaccaatc gagtgcttcc ggatattcgt cgcagtccag gacgaactcc
     2041 tggccccggc ctacggtctg cagcaataca tgatccagcc ccgccgccca gcgctgcaag
     2101 ttgcggtgat aactgagagc ggatcgcccg gccccaggat acatccatgc cggtaattgc
     2161 cagatactac gcgggctccc cggcgccgtt aaacataagt cggggtgaaa gcaggggaag
     2221 atccccgccc ccggtttatc caccgtggca ttcggcagca caatccggtc agtggagatg
     2281 tagagcgagt cgagcttatc gcgctgcgcg cggcaacaat gcgcgtggtc tcgcgcccag
     2341 accgggatgc gcgcccggtc atcgaccggg atgcgttgct cgatttgcag ccagccaaag
     2401 atgacatgcc ggcccggatc ctcgggcaca tagcgataac ggcctgcctg ttccacaacg
     2461 ttgcggaacc agccgtaaaa gataaaaatg tcgcccgcgc cgacgccctg gttctggaga
     2521 tggcgctccg ccgcgccggc ctgcccgaac agcggtttcc accccggcaa ccggggcaca
     2581 ctgtccagcg cgagatcggg atccaggtgg accgggcaat cgggtgg
//
LOCUS       DASUYC010000785         2367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1843693, whole genome shotgun sequence.
ACCESSION   DASUYC010000785 DASUYC010000000
VERSION     DASUYC010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1843693"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="VF276_12850"
     CDS             complement(<1..672)
                     /locus_tag="VF276_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016875429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX6607791.1"
                     /translation="MLIEERNTQQITPDAIAALAVQLRGSLLQPGSAAYEEARAVYNA
                     MIDRHPALIVQPMDVADVIAAVNFARSAGVPLAVRGGGHSGPGLGTVDDGVVIDLRLM
                     RGIHVDLAARTVRVEGGATWSDVDHATHPFELATPSGFISSTGVGGLTLGGGIGYLAR
                     TFGLTLDNLLGVDIVLADGRLVQANAAENPDLFWAVRGGGGNFGVVTSFLFRLHPVGT
                     VYGGPM"
     gene            complement(1174..1770)
                     /locus_tag="VF276_12855"
     CDS             complement(1174..1770)
                     /locus_tag="VF276_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607792.1"
                     /translation="MADMRRVVRDEETVVAGAPAQETVVTQTPGAPVAPVAPGTPVAP
                     VQPVPQTTVATTTPPPGDHVVARTASEAVVDPAADRAATVDWVGRLVWFLVAVLEVLL
                     ALRFVLLLTGANEGTGFAQLIYGVTRPFVAPFAGLFGSNMTYPGAAGTGVFAPETLVA
                     MVVYALIGFAIVKIAQLMLGTNQTRGTVVSDVNRRTRI"
     gene            complement(2017..>2367)
                     /locus_tag="VF276_12860"
     CDS             complement(2017..>2367)
                     /locus_tag="VF276_12860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6607793.1"
                     /translation="ADLPALYAGATLLAYPTRYEGQGLPPLEAMAAGTPVLASSTPAV
                     AEVAAPSALLVDPADEPGWAAALGRLLPDPALRAAMAARGRAVAARFTWPHCAAATLD
                     VYRIVLRSTRKMRQ"
BASE COUNT          404 a          786 c          807 g          370 t
ORIGIN      
        1 catcggcccg ccgtagacgg tcccgaccgg gtgcaggcgg aacaggaagg aggtgaccac
       61 gccgaagttg ccgccgccgc cgcgcaccgc ccagaacagg tcggggttct ccgcggcgtt
      121 ggcctgcacc agccgcccgt ccgccagcac gatatcgacg ccgagcaggt tgtcgagcgt
      181 cagaccgaag gtccgcgcca gatagccgat gccgccaccg agggtcaggc ccccgacccc
      241 cgtgctggag atgaagccgc tgggcgtggc caactcaaag ggatgggtgg cgtggtcgac
      301 gtcgctccag gtggcgcccc cttcgacgcg caccgtgcgc gccgcgaggt ccacatggat
      361 gccgcgcatg agccggaggt cgatcaccac cccgtcatcg acggttccca gccctggacc
      421 gctgtgcccg ccgccgcgta ccgcgagggg cactccggcg ctgcgggcga agttcacggc
      481 ggcgatcacg tcggccacgt ccatcggttg gacaatcagc gcggggtgcc ggtcgatcat
      541 tgcgttgtag acagcgcggg cctcttcgta ggcagcgctg ccgggctgca agagggaacc
      601 ccgcagttgg acggccagcg ccgctatggc gtccggcgta atctgctgcg tattgcgttc
      661 ttcgattagc atttgattct cctttgtggt gctctcgtca ggtaggctca ctcgcggccc
      721 ggcgttctcc gaacggcgcg tgatattagc gtatcaggcc gggtgttagc cgagcgttag
      781 gatgcgttag cggcgtgccc tggcattttg ccagcacgga ccggctgcga atgccacacg
      841 tctcaccgtg acaacacgcc gcgctcccac cgcttattct cgggctgccg gcacacagcg
      901 ccggcactag tgcctggtgg cgcaagcgga accactatgc atcacgcttc ccagcatact
      961 gcactgtctg gtgaaggaca tcgggagggt tccccatatt ggcgcgcccc cttccccatt
     1021 ttggaggcgt gcggagacct gcccatgcat ttgatagagc ggggattggt tggagggata
     1081 atcggatgac aaacaccggg ccggagaatg tccggcccgg tgtgcttata ggctatactt
     1141 atctgtctgc cgagggcggg gaaccgctgg cgactagatc cgcgtgcggc ggttcacatc
     1201 gctgaccacg gtgccgcggg tctggttggt gccgagcatc aactgggcga tcttgacgat
     1261 cgcgaagccg atcagggcgt agaccaccat cgccacgagc gtttccggcg cgaagacgcc
     1321 ggtgcccgcc gcgcccggat aggtcatgtt cgagccgaac agcccggcga acggcgccac
     1381 gaacgggcgg gtcacgccat agatcaactg cgcgaacccg gtaccctcat tggccccggt
     1441 cagcaacagc acgaagcgca gggcgagcaa cacttccagc acggccacca ggaaccagac
     1501 caagcgacct acccaatcca cggtcgccgc gcggtccgcc gccggatcga cgacggcttc
     1561 gctggcggtg cgcgccacaa catggtcgcc cggcggcggg gtggtagtcg ccacggtggt
     1621 ctgcggtacc ggctgcaccg gcgccaccgg ggtgccgggc gccaccgggg cgaccggcgc
     1681 gccgggggtc tgcgtcacga ccgtctcttg cgcgggcgcg ccggcgacaa ccgtctcctc
     1741 gtcccgcacc actctcctca tatcagccat ttgaaacctc ctccaggata ggctcctggt
     1801 tcaggggcaa gtccccttgg cgcctcattc gcccccgata tgccaacaac accggcggac
     1861 ctccggcgcc atggagacct gcattactaa tgcgccctaa ttaacagatc attactgccg
     1921 caagtgctaa tcgtttcagg attgagcgcg gctacggtat ataaagcaag gaccgtgcca
     1981 catcaagcca actgaaccgt agaaaatggg ggtgcgctat tgccgcattt tgcgggtgga
     2041 gcggaggact atacgataga catccagggt ggcggcagcg caatgcggcc aggtgaagcg
     2101 agcggcgacg gcgcgccccc gcgcggccat ggcggcgcgc agggcggggt cgggcagcag
     2161 gcggcccagg gcggcggccc aacccggctc gtcggcggga tcgaccagca gggcggaggg
     2221 cgcggcgacc tcggcgacgg cgggggtgct ggaggccagc acgggcgtgc ccgcggccat
     2281 cgcctccagc ggcggcaggc cctggccttc gtagcgagtg ggataggcca gcagcgtggc
     2341 cccggcgtag agcgcgggca ggtcggc
//
LOCUS       DASUYC010000786         3122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1594890, whole genome shotgun sequence.
ACCESSION   DASUYC010000786 DASUYC010000000
VERSION     DASUYC010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1594890"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VF276_12865"
     CDS             complement(<1..322)
                     /locus_tag="VF276_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010973723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX6607794.1"
                     /translation="MPATTPFLQLLTGSFSTPAAGNPTVAIVEAAYAHHGIPARYINC
                     DVAPAHLGDAVRGALAMGWIGFNCSLPHKQTVIPYLDDLGQSAALIGAVNCVVRRGDL
                     LTGEN"
     gene            complement(399..1322)
                     /locus_tag="VF276_12870"
     CDS             complement(399..1322)
                     /locus_tag="VF276_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6607795.1"
                     /translation="MSLAEKSTAAPAAAEHIMRPTSNLWRDAARRFFRNKLAVSALVV
                     MVLLILTAIFADVIAPAPYDYSVLAEANQFPNAHHLLGTDSVGRDFLSRLIYGARVSL
                     IVGFSVQLIALLIGVPLGTLAGMFGGWVDYVIMRWVEVLTAIPIWLFALFLISVWRSG
                     DNAPGNGLLNVVVAIGLIGWVDICRLTRAQLLALREKEFVTGAYAIGATRLHVALYHL
                     LPNALTPLIVAVTLGVPTAIFTEAGLSFLGFGINDPLPSWGKMVADSYAYIRVYWHLG
                     LLPTILIAVTMLSFSFVGDGLRDALDPRLNK"
     gene            complement(1368..2300)
                     /locus_tag="VF276_12875"
     CDS             complement(1368..2300)
                     /locus_tag="VF276_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6607796.1"
                     /translation="MVAYLIRRIIGLVIVFLIVSVLAFLLMHSVPGGPFDEEKSPLPP
                     AGKANILRKYGLDKPLYEQYLRYMGSALQGDFGISFQSPTESVLQLIGRTWPTSMQLG
                     GLAILFAFSTGLTFGIISAIRQNTWIDYLVTFVSTLGITVPSFVIAIWLLLIFSVQLH
                     WLPTGGWPGNGGDWTAAILPVLTLALGPSALVARYTRASLVEVIRADYIRTAQAKGLS
                     EQRIVLGHALKNALIPLITILAPQIPSLVTGTIFVEVIFRVPGLGKFFVSSIYLRDYP
                     MIMATMLLVAVLWSITYLLSDILYTVVDPRIRLQ"
     gene            complement(2382..>3122)
                     /locus_tag="VF276_12880"
     CDS             complement(2382..>3122)
                     /locus_tag="VF276_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6607797.1"
                     /translation="IVDRDTLIAQIIKPSQGIPAYSFLMPGFPASNSQGLKDIQNYDV
                     AKAKQLLAEAGYPDGKGFPKLTLWLRNEAPVRRDVAQAIAAAIKQNLGIDVEVSNKET
                     KTFTDAMNAKPTQIQFGMVSYGMDFLDPYNMLSVWLSGGRHNWNNSQFDDMVKKAAAF
                     TGDPAERVKMFQDAEKLLVTEAPGVFIYHRYQSDIYKPYLKGTELEPDQNGVAAIHWP
                     GFANTDTVIGSMYISKDVASANRKIPSP"
BASE COUNT          536 a          986 c         1098 g          502 t
ORIGIN      
        1 tgttctcgcc ggtgagcagg tcgccacggc ggaccacgca gttgaccgcg ccgatcagcg
       61 ccgccgactg gcccaggtcg tccaggtacg ggatgacggt ttgcttgtgc ggcagcgagc
      121 agttgaagcc gatccagccc atggcgagcg cgccgcgcac ggcgtcgccc aggtgggccg
      181 gggccacgtc gcagttgatg tagcgcgcgg ggatgccgtg gtgggcgtag gccgcctcga
      241 cgatggcgac agtcgggttg ccggcggcgg gcgtcgagaa cgacccggtc agcagttgga
      301 ggaaaggcgt ggtggcgggc atggcggttc ctttcagggg cggcgcgccg gcagacaagc
      361 ctcgcccgac cccgattcgc ggcggcccgg cctggcgatc acttgttcag gcgcgggtcg
      421 agcgcgtcgc gcaggccgtc gccgacgaag gagaagctga gcatggtcac ggcgatcagg
      481 atggtgggca gcaggcccag gtgccagtag acccggatgt aggcgtagga gtcggccacc
      541 atcttgcccc aactgggcag cgggtcgttg atgccgaagc ccaggaagct cagcccggcc
      601 tcggtgaaga tggctgtggg cacgcccagg gtgacggcga cgatcagcgg ggtcagcgcg
      661 ttgggcagca ggtgatacag cgccacatgc agccgcgtcg cgccgatggc gtaggcgccc
      721 gtgacgaatt ctttctcgcg cagcgccagc agttgggcgc gggtgagccg gcagatgtcg
      781 acccagccga tcagcccgat cgccacgacc acgttgagca ggccgttgcc gggcgcgttg
      841 tcgccgctgc gccagaccga gatcaggaac agcgcgaaca gccagatcgg gatcgccgtc
      901 agcacctcga cccagcgcat gatcacgtaa tcgacccagc cgccgaacat gcccgccagc
      961 gtgcccagcg gcaccccgat gagcagggcg atgagctgca ccgagaagcc gacgatgagc
     1021 gagacgcgcg ccccgtagat caggcggctc aggaagtcgc ggcccacgct gtcggtgccg
     1081 agcaggtggt gagcgttggg gaactggttg gcctcggcca gcaccgagta gtcgtagggc
     1141 gcgggcgcga tcacatcggc gaagatcgcc gtcaggatca gcagcaccat gaccaccagg
     1201 gcgctgacgg ccagtttgtt gcggaaaaag cgccgcgccg cgtcgcgcca gaggttcgag
     1261 gtcgggcgca tgatatgctc ggccgccgcc ggggccgccg ttgatttctc cgccagactc
     1321 ataccggtct ctccccagtc atcccggtgc cccctgcccg gtggccgcta ctgcagccgg
     1381 atgcgcgggt ccacgacggt gtagaggatg tcgctcagca ggtaggtaat gctccagagc
     1441 accgccacca gcagcatcgt cgccatgatc atcgggtagt cgcgcagata gatgctggac
     1501 acgaagaact tgcccagccc cggcacgcgg aagatcacct ccacgaagat ggtcccggtg
     1561 accaggctcg ggatctgcgg cgccaggatg gtgatcagcg ggatgagggc gtttttgagc
     1621 gcgtggccga gcacgattcg ctgttcgctc aaacccttgg cctgggcggt gcggatgtag
     1681 tcggcgcgga tcacctcgac caggctggcc cgcgtgtagc gcgccaccag ggccgacggc
     1741 cccagcgcca gggtgagcac cggcaggatc gcggccgtcc agtcgccgcc gttgcccggc
     1801 cagccgccgg tgggcagcca gtggagttgc accgagaaga tcagcagcag ccagatcgcg
     1861 atcacgaagc tgggcacggt gatgcccagc gtcgagacga acgtgaccag atagtcgatc
     1921 caggtatttt gccggatggc ggaaataatg ccgaaggtca ggccggtgct gaaggcaaac
     1981 agaatcgcca gcccgccgag ttgcatgctc gtgggccagg tgcgcccgat cagttggagc
     2041 accgactcgg tcgggctctg gaacgaaatg ccgaaatcgc cttgcagcgc gctacccata
     2101 tagcgcaggt actgttcata cagcggcttg tcgagcccgt acttgcgcag aatattggcc
     2161 ttgcccgcgg gcggcagcgg cgacttctcc tcgtcgaacg ggccgcccgg caccgagtgc
     2221 atcagcagga aggccaggac cgagacgatg agaaagacga tgaccaggcc gatgatgcgg
     2281 cggatcaagt aggcaaccat gctccggccc cttgccgcgc cgggggcggg ggcgggcagc
     2341 cgcggcccta cccccacccc cggcgcctga tacgccccgc cttacggcga cgggatcttg
     2401 cggttggcgc tcgctacgtc cttcgagata tacatcgagc cgatgaccgt atcggtgttg
     2461 gcaaagcccg gccagtggat ggccgccacg ccgttctgat ccggttcgag ctcggtgccc
     2521 ttcaggtagg gcttgtagat gtcgctctgg tagcggtggt agatgaagac gcccggcgcc
     2581 tccgtcacca gcagcttctc ggcgtcctgg aacatcttga cgcgctcggc ggggtcgccg
     2641 gtgaacgcgg cggccttctt caccatgtcg tcgaactgcg agttgttcca gttgtgccgc
     2701 ccgccgctca accacacgct cagcatatta taggggtcga ggaagtccat gccataggag
     2761 accatgccga actggatctg cgtcggcttg gcgttcatgg cgtcggtgaa cgtcttggtc
     2821 tccttgttcg agacctccac gtcgatgccc aggttctgct tgatcgcggc ggcgatggcc
     2881 tgggccacgt cccggcgcac gggcgcctcg ttgcgcagcc acagggtcag cttggggaag
     2941 cccttgccgt cggggtagcc ggcctcggcg agcagttgct tggccttcgc cacgtcgtag
     3001 ttctggatgt ccttgaggcc ctgcgaattc gacgccggga agccgggcat caggaacgag
     3061 taggcgggga tgccctggct cggcttgatg atctgggcga tcagcgtgtc gcggtccacg
     3121 at
//
LOCUS       DASUYC010000787         2323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1740116, whole genome shotgun sequence.
ACCESSION   DASUYC010000787 DASUYC010000000
VERSION     DASUYC010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1740116"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..1273)
                     /locus_tag="VF276_12885"
     CDS             complement(71..1273)
                     /locus_tag="VF276_12885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6607798.1"
                     /translation="MRVALVHDYLNQYGGAEKVLEALHDLYPNAPVYTSVYAPDSMPA
                     HFRDWTIHTSFMRHLGVFRRRHQLGLLLYSTAFERFDLSRYDLVISSSSAWAKGVITG
                     PHTLHVCYCHAPMRFVWHYHEYLRGESIGPLGRMILPFILGYVRLWDEVSAQRADSYI
                     ANSREVARRISKYYRRTATVINPPVDLSNYEVAPSYDDYFLVVSRLVPYKRLDIVIEA
                     FNQMGLPLKVVGRGRQREALESAAGPNIEFLGAVTQEELRRLYARCRALVWAEAADFG
                     IAPVEVQASGRPVIAYAAGGALETVVAGETGCFFHQQTAEALIAAVQSFDAARFDPAR
                     IRSHAEQYDVSVFKRRMAEYIDAALADRHQYEGPHARLNGQYAPAQDGDETLVHSSRE
                     PGAYSLPS"
     gene            complement(1319..>2323)
                     /locus_tag="VF276_12890"
     CDS             complement(1319..>2323)
                     /locus_tag="VF276_12890"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX6607799.1"
                     /translation="NIRRVLVVGASYAGQRIMKDLMERPDLGYALSGYVEDDTDEAWT
                     VPMNGHGYPARLGRVTDVDQVISAGQIDEVIVALPATSHNQIVEVIERCQSNHVSFRL
                     IPDLFEIRFNQVQIDALNGIPLIGLKEVALRGGNLWLKRTLDILMAMVVIAVTSPIML
                     LIAILIKLERPRGPVFFKQERVGYGGRRFLCYKFRSMRPDAEQLKDQLMAYNEASGPI
                     FKMKNDPRVTRVGRWLRRTSLDEIPQVFNILAGDMSWVGPRPPVPSEVEQYNEWHRRR
                     LEITPGLTGLWQVSGRSDLSFDEMVKLDLYYAENWSLGFDIKIILMTIPAVLKARGAY
                     "
BASE COUNT          414 a          752 c          741 g          416 t
ORIGIN      
        1 cctgtcgggg aatgtctgct ctaccggaac ggcgcgataa atcgcgcggc tacactgctg
       61 gagccggtat ctagctcggc aaactgtagg cgcccggctc gcgcgacgaa tggaccaggg
      121 tctcgtcgcc gtcttgcgcg ggcgcgtact ggccgttcaa ccgggcgtgc ggtccttcat
      181 actggtggcg gtcggccagc gccgcgtcga tgtactcggc catgcggcgc ttgaacacac
      241 tgacatcgta ctgctcggca tggctgcgga tgcgggccgg gtcgaaccgg gcagcgtcaa
      301 agctctgcac cgccgcgatc agtgcctccg ccgtctgctg atggaagaag cagcccgtct
      361 cgccggccac caccgtctcc agcgcgccac ccgccgcgta ggcgatgacg ggacggcccg
      421 atgcctggac ctccaccggc gcgatgccga agtcggcggc ctcggcccag accagcgcgc
      481 gacagcgggc atagagccgg cgtagctcct cttgcgtcac ggcgcccagg aactcgatgt
      541 tggggccggc ggccgattcc aacgcctcgc gctgccgccc gcggcccacc accttgagcg
      601 gaagtcccat ctggttaaaa gcttcgatga cgatatccag gcgcttgtag gggaccaggc
      661 gcgagacgac caggaaatag tcgtcgtagc tcggggcgac ttcgtagttg gagagatcca
      721 ccggcgggtt gatcaccgtg gccgtgcgcc ggtaatactt gctgatccgc cgggcgactt
      781 cgcgtgagtt agcaatgtac gagtcggcgc gctgcgccga cacttcatcc cagagtctaa
      841 catagcccag gataaatggc aagatcatgc gccccagcgg cccgatgctt tcgccgcgca
      901 ggtactcgtg atagtgccag acgaagcgca tcggcgcgtg gcagtagcaa acgtgcagcg
      961 tgtgcggccc ggtgatgacg cccttggccc aggcgctgct gctgctgatc accaggtcgt
     1021 agcggctcag gtcgaagcgc tcgaacgcgg tggagtagag cagcagcccc aactggtgcc
     1081 ggcgccgaaa gacgcccagg tgccgcatga acgaggtgtg gatcgtccag tcgcggaagt
     1141 gcgccggcat gctgtcgggc gcatagaccg aggtgtacac cggtgcgttg gggtagaggt
     1201 cgtgtagggc ttccaggacc ttttccgcgc cgccgtactg gttgaggtaa tcatggacca
     1261 gggcaaccct catgccgcgt cctcattgcg catcccgcgc cagtgatccc gcacgatcct
     1321 aatacgctcc tctagccttg agcaccgccg gaatggtcat caagatgatc ttgatgtcaa
     1381 accccagcga ccagttctcc gcataataca ggtcgagctt caccatctcg tcaaacgaaa
     1441 gatcgctgcg tccgctgacc tgccacaggc ccgtcaggcc cggcgtgatc tccaggcggc
     1501 gccggtgcca ctcattatac tgctcgactt cactcggcac cggcgggcgc gggcccaccc
     1561 agctcatgtc gccggccagg atgttgaaca cctggggtat ttcgtccagg ctggtgcgcc
     1621 gcaaccaccg acccacgcgc gtcacccgcg gatcgttctt catcttgaag atcggcccgc
     1681 tggcttcatt atacgccatg agctggtctt ttagctgctc ggcatccggg cgcatcgagc
     1741 ggaatttata gcacaggaag cgccgaccgc cgtagccgac gcgctcctgt ttgaagaaga
     1801 cgggcccgcg cgggcgctcc agcttgatga ggatggcgat cagtagcatg atcggcgaag
     1861 tcacggcgat caccaccatc gccatgagga tgtcgagcgt gcgcttgagc cacaggttgc
     1921 cgccgcgcag ggccacttcc ttcagaccga tgagcgggat gccgttgagc gcgtcgatct
     1981 gcacctggtt gaagcgaatc tcaaacaggt cggggatcag gcggaagctg acatggttgc
     2041 tctggcagcg ttcgatcact tcgacgatct ggttgtggct ggtggccggc agggcgacga
     2101 tcacctcgtc gatctgccca gcactgatca cctggtcgac atcggtgacg cggcccaggc
     2161 gcgcggggta gccatggccg ttcatcggca cggtccaggc ctcatcggtg tcgtcctcga
     2221 catagcccga cagggcataa cccagatcgg gccgctccat caggtccttc atgatgcgct
     2281 ggccggcata gctcgcgccc accaccagca cgcgccggat gtt
//
LOCUS       DASUYC010000788         6532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_817404,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000788 DASUYC010000000
VERSION     DASUYC010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6532
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817404"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2124)
                     /locus_tag="VF276_12895"
     CDS             complement(<1..2124)
                     /locus_tag="VF276_12895"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX6607800.1"
                     /translation="MKGPIRLRRRAFAAWALTALLALSVLDLTGMIAPGLAAGQVAPA
                     ARGPVTIAPEVLRATANGASAPIVILLAEQADLSSAYNMRDQDARGWFVYNTLRDTAA
                     RTQGPLQAFLRAQGRTYQSFWVANVLMTTGDRTLIDALAARDDVRAIESNAPFAAITD
                     PVRAPQGESTAPAVPGAVEWGVANVNAPAVWAMGYTGQGIVIANQDTGMLWDHAALKG
                     HYRGWNGTTADHNYNWHDSVHSGGGICGANSPFPCDDDGHGTHTTGTVIGDDGGANQI
                     GVAPGAKWIGCRNMDQGTGTPATYTDCFQFFIAPTDLNGQNADPTRRPHVMNNSWGCP
                     PSESCAATTLQTIVENTEASGIFVEASAGNSGSSCSSVSDPPAIYSAAFSTGAYNSSN
                     TLASFSSRGPVTVDGSNRRKPDIAAPGVNVRSAYYSGTTSYASLSGTSMAGPHVVGVV
                     ALLWSAHPELVRQIAATKALLEQTANPTVLVTTGATSCGGIAYTTIPNNHFGYGRIDA
                     LAAVNGAATPTPTVTGTPPTATATATPVPPTATRTAVPPTVTVPPATSTALPATATET
                     PTGVAATTTPAPPTVTPPAGATATATAPAATATPVNCPLPFTDVDTFNPFYQYIQCLY
                     CRGIISGYSDNTFRWGVDVTRGQVSKIIANSAGLNAAVSGQTFTDVPASNPFYVYIER
                     LAATGAISGYDTAANCPTGTPCFRWE"
     gene            2568..4142
                     /gene="rny"
                     /locus_tag="VF276_12900"
     CDS             2568..4142
                     /gene="rny"
                     /locus_tag="VF276_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015909284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEX6607801.1"
                     /translation="MPDLIWWGITVLLLVVEAIGIAVYLTRKTAVATQQQIALVEKDA
                     ADRIRQAQEETQRAQEDAKTRQKEMVLQAQEERLRMRTEIEAEFRDQRVQLQTVEQRL
                     QRKEETLDRKIEALDSRERKIQAREKESEQVKAQLENAQQEQQRTLERIAGMSADEAA
                     ARLMQQVEQEVAQDVARRARELELQLKEEADIRARKIISIAIARAASDYVPEITVTTV
                     PLPNEEMKGRIIGREGRNIRAIERATGVDLIIDETPESITLSAFDPVRREVARRTLQK
                     LIADGRIHPARVEEVVAKVQQEMDVHIREEGERAAYEANVQGLHPDLIKLLGRLSFRT
                     SYGQNQLHHAIESSRIAAAIAAEIGADINVAKTATLLHDIGKAVDHEVEGPHALIGAD
                     IARRLGRSQKIVHAIAAHHSEEEPQTVEAWIVQAADALSGGRPGARRDSVEHYTKRLE
                     ALEGVANSFEGVERSYAIQAGREVRILVKPDRIDDLGAMRLARDIVKKIEETLEYPGQ
                     IKVTVIRETRAVDFAK"
     gene            4544..4858
                     /locus_tag="VF276_12905"
     CDS             4544..4858
                     /locus_tag="VF276_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HEX6607802.1"
                     /translation="MHSAPSLDGALEAPDDGEEVLKVSAHSRPSAVAGAIAGVVRHAG
                     RAEVQAIGAGATNQAIKAVAIARDYLTPDGIDIVCVPAFIDVTIANEERTAIRLVIER
                     RG"
     gene            5007..5858
                     /locus_tag="VF276_12910"
     CDS             5007..5858
                     /locus_tag="VF276_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX6607803.1"
                     /translation="MGKLDLHTHTTASDGRLAPAALVALAAQVGVTTLAVTDHDTTDG
                     LTEARAATGAAGVEIITGIEFGCDMPHGEIHMLGYLFDATHPALRARLAWLREGRVER
                     GRAMVAKLNALGLPIRWERVREIAGAGSVGRPHVAQALIEGGYVADTNEAFSRYLAWG
                     GPAYVPRRRLTPLDVIALIREAGGVVSLAHPAHIPDLEAQLAPLAEAGLAGLETYYGE
                     YDAPTVTWLAELAARFHLVPTGGSDYHARDIKDHAMLGAGPPVPPDTIARLRDRLPAA
                     PAPEARP"
     gene            5855..6343
                     /locus_tag="VF276_12915"
     CDS             5855..6343
                     /locus_tag="VF276_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017360428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX6607804.1"
                     /translation="MTLPAHDYLETRGIPHERRSFPVEIEKGAASVARALGFRERQMV
                     KTLIFETDQGERVLIMLGGDQNAISGHLKKALGSRNIRMASPEAVISTTGYAIGSIPP
                     FHWQPAGFRSFLEESLLAEPILGVGAGQWGEEILITPAHLIAATGAQVVNLTDRDRPM
                     QL"
BASE COUNT         1125 a         2215 c         2195 g          997 t
ORIGIN      
        1 ttcccagcgg aagcaggggg tgccggtggg gcagttggcc gctgtatcgt agccgctgat
       61 cgccccggtc gccgccaacc gctcgatgta gacatagaac gggttgctgg cgggcacgtc
      121 ggtgaacgtc tggccgctga ccgccgcgtt cagccccgca gagttggcga tgatcttgga
      181 gacctgaccg cgggtcacat ccacacccca gcggaaggtg ttgtcgctat agccgctgat
      241 gatgccccgg cagtagagac actggatgta ctggtagaac gggttgaagg tgtccacatc
      301 cgtgaacggc agggggcagt tcaccggcgt cgccgtggcg gcgggcgcgg tcgccgtggc
      361 cgtcgcaccg gccggcggcg tgaccgtcgg cggcgccggg gtggtcgtcg ccgcgacgcc
      421 ggtgggcgtc tccgtcgcgg tggccggcag cgcggtcgat gtcgcaggcg gcacggtgac
      481 cgtcggtggg acggcggtac gggtggccgt cggcgggacg ggggtggccg tggcggtggc
      541 ggtcggcggc gtgccggtca cggtcggggt gggcgtggcg gcgccgttga cggcggccag
      601 ggcgtcgatg cgtccgtagc cgaagtggtt gttcgggatc gtggtgtacg caatgccgcc
      661 gcaggacgtg gcgcccgtcg taaccagcac ggtcgggttg gccgtctgct ccagcagcgc
      721 cttcgtcgcg gcgatctggc gcaccagctc aggatgggcc gaccagagca gggccaccac
      781 gccgacgacg tgcggcccgg ccatcgacgt gcccgacagg ctggcgtagc tcgtcgtgcc
      841 cgagtaatag gcggagcgca cattcacgcc cggcgccgcg atgtcgggct tgcggcggtt
      901 ggagccgtcc accgtcaccg gcccgcggct gctgaagctc gccagggtgt tgctgctgtt
      961 gtaggccccg gtgctgaacg ccgcgctgta gatggcgggc ggatcgctga cggaggagca
     1021 ggagctgccg ctgttgccgg cggaggcttc gacgaagatg ccggacgcct cggtgttctc
     1081 cacgatcgtc tgcagggtcg tcgccgcgca gctctcactg ggaggacagc cccagctatt
     1141 gttcatcacg tgcgggcgcc gggtcgggtc ggcgttttgg ccgttcaggt cggtcggcgc
     1201 gatgaagaac tggaagcagt cggtatacgt ggccggggta ccagtgccct ggtccatgtt
     1261 gcggcagccg atccacttgg cgccgggggc cacgccgatc tggttcgcgc cgccgtcgtc
     1321 gccgatcacg gtgccggtgg tgtgggtgcc gtggccgtcg tcgtcgcagg ggaacggcga
     1381 attggcgccg cagataccgc cgccgctgtg gacggagtca tgccagttgt agttgtggtc
     1441 ggcggtggtg ccgttccagc cgcgataatg ccccttgagg gccgcgtgat cccagagcat
     1501 gccggtgtcc tggttggcga tgacgatgcc ctggccggta tagcccatcg cccagaccgc
     1561 cggcgcgttc acattcgcca cgccccattc gaccgcgccc ggcaccgccg gagccgtgct
     1621 ctcgccctgc ggcgcgcgca ccgggtccgt aatggcggcg aagggcgcgt tcgactcgat
     1681 ggcgcgcaca tcgtcccgcg cggcgagcgc gtcgatcagg gtgcggtcgc ccgtcgtcat
     1741 aaggacgttc gccacccaga acgactggta ggtgcggccc tgggcgcgca ggaaggcctg
     1801 gagcggtccc tgcgtgcgcg ccgccgtgtc gcgcagggtg ttgtagacaa accagccgcg
     1861 ggcatcctgg tcgcgcatgt tgtacgcgct gctcaggtcg gcctgctcgg ccagcagaat
     1921 cacgatcggg gcgctggcgc cgttcgcggt ggcacgcaat acctcggggg caatcgtgac
     1981 cggcccgcgc gccgcgggcg ccacctggcc ggcggccagg cccggcgcga tcatgccggt
     2041 gagatcgagc accgacagcg ccagcaaggc cgtcagcgcc cacgcggcga atgcccgccg
     2101 ccggagtcgt atgggtccct tcattacggt ttgtgttact ccttccctgt gataaaaccg
     2161 ctagatcgcc gcacaatggg ggttgcgagc tgtcccgaag gcgccgcggc aggcaaacaa
     2221 agggggcggg acataggcgc atattatatg aaatggcctg cgtctgtcaa atgtcgagat
     2281 cggcgggccg gtccccggtc gggtttgtcg gaaatcgaga caaaatcacc atcttgacag
     2341 gcgtatataa agggtatatc atggtagcca aatcatgctg ccacacctta ccaccggcgt
     2401 ggcggcaacg ggttcgcatg tagccagggc cggcacccca ggcagccgcc ggtttgcctg
     2461 ccgggcgcgt atccagcgga tacgcggttc ggggccggac gcggtcctgc ttcgttatca
     2521 ccgccttagt ccatcgaacc ttaaaaactg cataggagga gtaacagatg ccagatctca
     2581 tctggtgggg tatcaccgtg ttactgctgg tggtggaagc catcgggata gcggtctacc
     2641 tcacccgaaa gacggcggtc gcgacgcagc aacagatcgc tctcgtcgag aaagacgcgg
     2701 ccgaccgcat ccgccaggca caagaggaga cccaacgcgc ccaggaagac gccaaaaccc
     2761 ggcaaaaaga gatggtgctg caagcgcagg aagagcgact acgcatgcgc accgagatcg
     2821 aagccgagtt ccgcgaccag cgagtccaac tccagaccgt ggagcagcgg ctccagcgca
     2881 aagaagaaac cctcgaccgc aagatcgagg cgctggatag ccgggagcgc aagatccagg
     2941 cgcgggagaa agaaagcgag caagtcaaag cgcagctcga aaacgcccag caagaacagc
     3001 aacgcaccct ggaacgcatc gccgggatgt ccgccgacga ggcggcggcc cgcctgatgc
     3061 aacaggtcga gcaagaagtc gcacaagatg tcgctcgccg cgcccgggaa ctggagcttc
     3121 aactcaagga agaagccgac atccgggcgc gcaagatcat ctccatcgcc atcgcccgcg
     3181 cagcgtcgga ctacgtgccc gaaatcaccg tcacgacggt cccgctgccg aacgaagaaa
     3241 tgaaaggccg catcatcggc cgggaggggc gcaacatccg cgccatcgaa cgggccaccg
     3301 gggtcgacct gatcatcgat gaaacaccgg aatccatcac gttgtcggcc ttcgacccgg
     3361 tccgccggga agtcgcccgg cgcaccctgc aaaaactgat cgccgacggc cgcatccacc
     3421 ccgcgcgcgt cgaagaagtg gtcgccaaag tccagcagga gatggatgtc catatccgcg
     3481 aggaaggcga acgggcggcc tacgaggcca atgtccaggg cctgcacccg gatctgatca
     3541 aactgctggg ccggctctcc ttccggacta gctacggcca gaaccagttg caccacgcca
     3601 tcgagtcgtc gcgcatcgcc gcggcgattg ccgcggaaat cggggcggat atcaacgtcg
     3661 ctaaaacggc tacgctgctc cacgatatcg gcaaggccgt agaccacgag gtcgaaggac
     3721 cgcacgcctt aatcggggcc gatatcgccc ggcggctggg ccgttcgcag aaaatcgtcc
     3781 atgccatcgc cgcccaccac tccgaggagg agccgcaaac ggtcgaagcc tggatcgtcc
     3841 aggccgccga tgcgctctcc gggggccggc ccggcgcccg ccgcgacagt gtcgaacact
     3901 acaccaagcg gctcgaagcg ctagaaggag tcgccaattc gttcgaggga gtcgagcgct
     3961 cgtatgcgat ccaggccgga cgcgaagtcc gcatcctggt caaacccgat cggatcgacg
     4021 atctcggcgc gatgcggctg gcccgcgaca ttgtgaagaa gatcgaggaa actcttgaat
     4081 atcccggcca aatcaaagtg accgtgatcc gtgagacacg ggccgtcgac tttgccaagt
     4141 aaccggctac tctccgccgt cgcccctgcg gtcggggcgc ggtggcggag gggggttcag
     4201 gggtccgccg taggaggccg gcacgcccca tcactacacc aggcacccgc catcgaccag
     4261 tgaactgcgc gttaacctct tcttcgtttc cccgccccca ccggctgccg ccgcgtgggt
     4321 tgtatccacc accggcgggc cggcactgac cggctgtgca aaggagcagt gtaaatctat
     4381 gggccgtatc tacgatgacc tcaacctcaa agacatcaag atcgaacccg gtgttcgttt
     4441 ccctcccgaa cccgacaccg aaagccggag ggcctcgaca ctcaagctgg agactcctat
     4501 ggaccggacc cacgagaccc tgcgcgccgt ccccctgcag cccctgcata gtgcgcccag
     4561 cctggatggc gccctggaag cccccgacga cggggaagaa gtcttaaagg tatccgcgca
     4621 ttcgcgcccc agcgccgtgg ccggcgccat cgccggggtc gtccgccacg ccggtcgggc
     4681 cgaggtgcag gcgatcgggg cgggcgccac caaccaggcg atcaaagcgg tcgcgattgc
     4741 ccgtgattac ctgacccccg acggcatcga catcgtctgc gtgccggcat tcatcgatgt
     4801 caccatcgcg aacgaagaac ggaccgccat tcgcctggtg atcgagcggc gcggctaacc
     4861 cccgcggcgc acccacccgg atcccgtgta ccggtgtagt gcctcctgtg ccggtcgccg
     4921 gatactgagt aaaccccgcc cttgcggtct ccccgccagg ccggggtttt tgttttgccg
     4981 ccaaccgacc aagcgaaagg attctcatgg gcaaactcga tctgcatacc cacacgaccg
     5041 cttccgatgg gcgcctggcg cccgccgcgc tggtcgccct ggcggcccag gtgggcgtca
     5101 ccaccctcgc cgtcaccgac catgacacga cggacggtct gaccgaggcc cgcgcggcga
     5161 cgggcgccgc gggcgtcgag ataatcaccg gcatcgagtt cggttgcgat atgccgcacg
     5221 gcgagatcca catgctcggc tacctgttcg acgccacgca cccggcgctg cgcgcgcggc
     5281 tggcctggct gcgcgagggc cgtgtcgagc gcgggcgggc catggtcgcc aagctcaacg
     5341 ccctggggct gccgatccgc tgggagcgcg tgcgggagat cgccggcgcc ggctcggtgg
     5401 gccggcccca tgtcgcccag gcgctgatcg agggcggcta tgtcgccgac accaacgagg
     5461 cgttcagccg ctacctggcc tggggcggcc cggcctatgt gccccgccgc cgcctcaccc
     5521 cgctcgatgt gatcgccttg atccgcgagg cgggtggggt cgtctcgctg gcccacccgg
     5581 cgcacatccc cgatctcgaa gcccagctcg cgcccctggc cgaagccggg ctggccgggc
     5641 tcgaaaccta ctacggcgag tacgacgcgc cgaccgtcac ctggctggct gaattggccg
     5701 cgcgcttcca cctggtgccc accggcggca gcgattacca cgcccgcgac atcaaggatc
     5761 acgctatgct cggcgccggc ccgcccgtgc cgcccgacac tatcgcccgc ctgcgcgacc
     5821 gcctgccggc agcccccgcc ccggaggccc ggccatgacc ctgcccgccc acgactacct
     5881 ggagacccgc gggatccccc atgagcggcg cagctttccc gtggagatcg agaagggcgc
     5941 cgcctcggtg gcccgcgcgc tcggcttccg cgagcgccag atggtcaaga cgctgatctt
     6001 cgagaccgac cagggggaga gggtgttgat catgctcggc ggcgaccaga acgccatctc
     6061 cggccatctc aagaaggccc tcggctcgcg caacatccgc atggcaagcc cggaggctgt
     6121 catctctacc accggctatg ccatcgggtc catcccgccc ttccactggc agcctgcggg
     6181 gttccggtcc ttcctggagg aatcgctgct ggccgagccg atcctgggcg tgggcgccgg
     6241 gcaatggggc gaggagatcc tgatcacccc ggcccacctg atcgccgcca ccggcgccca
     6301 ggtggtgaac ctgacggatc gcgaccgccc catgcagctc tgacaacgat atgcctaagg
     6361 agacacgatg atgcctggta ttaccagtga aaccgacgta ctcgcgcgcc tgaccggctg
     6421 ggccggcggc cagccgctga tccgcgcggt gctgctcacc agttctcgcg ccaacccgcg
     6481 cgcgcccctc gacatcctgt ccgactatga cgtgatcctg gtcgttgccg ac
//
LOCUS       DASUYC010000789         6066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_845603,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000789 DASUYC010000000
VERSION     DASUYC010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845603"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..672)
                     /locus_tag="VF276_12920"
     CDS             complement(16..672)
                     /locus_tag="VF276_12920"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX6607805.1"
                     /translation="MDVERYTATLSPRQRRIFLALDQYADAATGVLALLSAALQLLIG
                     TPGAVVLAAVALVLPAGLLTYGVNRLCKWLLPKPRRNRRYHDLISPWLRGSFPSFHTQ
                     FAATFGATFSMAVLLLAPAEARRLAGGVAALSMGLSVALVAWSRLYLAVHDRVDVYGG
                     LALGAALGAGIAAGAVALGAVPAGWAAWACVAGLVALVLAVSVWERRKRRPVRSMPRG
                     "
     gene            complement(680..1081)
                     /locus_tag="VF276_12925"
     CDS             complement(680..1081)
                     /locus_tag="VF276_12925"
                     /inference="COORDINATES: protein motif:HMM:NF012267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX6607806.1"
                     /translation="MTQVKRDLDSPPAPLPASEEEAVMAATPPLDRAAPVPQVKRQRV
                     VLELHVDPNICLSCAGCVSVCPRMAVPVSHFNSLAEGILCRGCGICVQVCPVEALSQV
                     PIILDEPLHVPGNRDRRRATPVTPPESDEAN"
     gene            complement(1106..2269)
                     /locus_tag="VF276_12930"
     CDS             complement(1106..2269)
                     /locus_tag="VF276_12930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6607807.1"
                     /translation="MLRSADYDVVIVGAGPAGANCARYAAAGGLRVLVLDKKAELGIP
                     TECSGAVSLRGLTDAGVPPSDEFVVSSIAGFLTYSNDGEPWRIDYREIAGRETIGVVV
                     DRRRLDTHVAGLATEAGAELALRTELEDAVRVDPGDPAAGWLLRGRHHGLPFEIRATV
                     LVAADGVGSRIAKWLRINTTLPLHEAASCIQYEMTNVPTDRLLEIVVGQDHAPGGYVW
                     VFPTAPGRAKVGLGVVRTMTRQPAGWHLDRFIASSFMRDRFTRASVLEVSIGTVPLAR
                     PMPTPYADGVIFIGDAARHVNSLTGGGIHTALIAGRVAGESLKALWPAGDYSQVGLAT
                     WGDAWEAQMGAGLREVYTLKTGVFSIIDPAAQNARLYDVLGGYFHPSSAHRKR"
     gene            2466..4451
                     /locus_tag="VF276_12935"
     CDS             2466..4451
                     /locus_tag="VF276_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607808.1"
                     /translation="MHRAIAGARVRRVVGPLLLCLVGALILWTAAWQVKRADTVDLGG
                     PQDGPFVQHFNTREPDPAKQPNPPYTYRWSQGTSTIFWPGTGNFPLTITLRLASVPLP
                     GAPQTVTATVRGQTFPLALANELRDYSFRVERGNPLEGDVAVTLTTTPFHGPGDPREL
                     GVLVDSATMAPIDTGVRPGVVPPPLDMARWAAVLALLYGLTWWLSGSTRAATATGIGV
                     ALFVAVEAIGERANLGFLAPEAFVLLLWAVPLAVVARWAARATLRLAAHAAGQPVRLP
                     AHGAAEAAAAALVAGWLLRIGGMVYPQFMSSDIGLHVNNIGKVLRGQWVWAGVLPNGA
                     PQPYPPLTYLTLAPFAGLVPDLSLLLRAGVSLLDAACVLPLLYLGTRLGGARAGAWAG
                     WVYALLPAPFTLFSAGVYANIFANIVFTWTLALWGELLLARRVTPALAAALAVGFFLT
                     FMGHYGMLIGAVAVAAAFIVLALLSGPPILRRRALIVAGTLAVAGLAAYLTYYQHFLD
                     VMLAQVRGVGAQVTPGGATDAAAGDVTGGAAAITPGALLNLLWRRLGPDLGAAALLAT
                     LAGWIAAGRAARGVQVLVVATALAGIGFAALSLRAGENIRYALLFAPLVAVGAGLFLS
                     RLDRRGPAGLWWSVALVAVLAWHLLEVWVPLIFTRYH"
     gene            4476..5285
                     /locus_tag="VF276_12940"
     CDS             4476..5285
                     /locus_tag="VF276_12940"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6607809.1"
                     /translation="MMRHDLYEDLYLKEQDYWWHVGKRAIVYSLLEKFLPPGDPQTRH
                     ALDLGCGTGHNLDSLARYAEPVGLDSSAEALAFCRRRGHQRLIQAASLPFADNSFDIV
                     TALDVIEHLDDDLGTLREVYRVMRPGGLLIISVPAYKVLWSYWDQILGHRRRYTTANM
                     ARVVRRAGWRVRKVSYSNLAILAPTVAVRLVKSLRHRKSEVAMPASDSAEHMETGGPE
                     TDFVDVPGWLNSAMITYYRGESRALRAVDLPFGLSVVCVAQKPRGSRPQPL"
     gene            5282..>6066
                     /locus_tag="VF276_12945"
     CDS             5282..>6066
                     /locus_tag="VF276_12945"
                     /inference="COORDINATES: protein motif:HMM:NF021379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HEX6607810.1"
                     /translation="MSKVSAAPALPHARLEGTRLDAALRFSAWVERQAPRLLVAAMAA
                     YVVILFAASVYKYEHFGQGYDQVDFEQALWNTVHGRPFADSRFNFTDSIFGMDWMPLL
                     AVFVPVYALLPSALPLFFLQILAAALGAWPVYLLARDKLGTRRHGLVFGLAWLLYPTI
                     QYSVLDPFQIRIFATTLLLCALLFFERARMIPFLITAGLALLARTDVALVVLMFGVYA
                     LLARRGVRWVIAPLALGGGYFLLVMTFIVPAFVHLPAVHCTGP"
BASE COUNT          820 a         2180 c         2131 g          935 t
ORIGIN      
        1 cgggccaggg cgcccctagc cgcggggcat ggaccggacc gggcggcgct tgcggcgctc
       61 ccagacgctc acggccagca ccagcgcgac tagcccggcc acgcaggccc aggccgccca
      121 cccggcgggc accgcgccca gcgccacggc gcccgcggcg atcccggccc ccagcgccgc
      181 gcccagcgcc agcccgccgt agacatcgac ccggtcgtgg acggccaggt agagccgcga
      241 ccacgccacc agggccaccg acaggcccat gctgagcgcg gccaccccgc ccgccaggcg
      301 ccgcgcctcc gcgggcgcca gcagcaacac ggccatgctg aaggtcgcgc cgaaggtggc
      361 ggcgaattgc gtgtgaaacg aggggaacga cccgcgcagc cagggcgaga tcaggtcgtg
      421 gtagcggcgg ttgcgacgcg gcttgggcaa tagccacttg cagagccggt tcacgccgta
      481 ggtcagcaac ccggcgggca acaccagcgc gacggcggcc agcaccacgg cgcccggcgt
      541 gccgatcagc agttgcagcg ccgccgagag cagcgccagc acgccggtgg cggcgtcggc
      601 gtactggtcc agcgcgagga agatgcggcg ctggcgcgga ctgagcgtcg cggtatagcg
      661 ctcgacatcc atcgcgccgt tagttggcct cgtccgactc gggcggagtg accggggtgg
      721 cccggcgccg gtcgcggttg cccggcacgt gcagaggctc atccaggatg atgggcacct
      781 ggctcagggc ctcgaccggg cagacctgca cacagatgcc gcagccgcgg cagaggatgc
      841 cttcggccag gctgttgaaa tggcttaccg gcacggccat gcgcgggcac acgctgacgc
      901 agccggcgca cgagaggcag atgttcgggt ccacatgcaa ctccagcacc acgcgctgcc
      961 gcttcacctg cggcaccggc gccgcgcgat ccaggggcgg cgtggcggcc atgaccgctt
     1021 cctcctcgga ggcgggcagc ggcgcggggg gcgagtccag gtcccgtttt acctgggtca
     1081 tagcaaacct ctttcgtccg gcatttcagc gtttgcggtg ggccgagctg ggatggaagt
     1141 agccgcccag cacgtcgtag agccgggcgt tctgggcggc gggatcgatg atactgaaga
     1201 cgccggtttt caaggtgtag acctcgcgca gcccggcacc catctgggcc tcccaggcgt
     1261 cgccccaggt cgccaggccg acctgcgagt agtcgcccgc gggccacagc gccttcaggc
     1321 tctcgccggc cacccgcccg gcaatgaggg cggtgtggat gccgcctccg gtcagcgaat
     1381 tcacatggcg cgcggcatcg ccgatgaaga tcacgccgtc ggcgtagggc gtgggcatgg
     1441 gccgggccag cggcacggtg ccgatggaca cttcgagcac cgaggcgcgg gtgaaccggt
     1501 cgcgcatgaa cgacgaggcg ataaaacggt cgaggtgcca gcccgccggc tggcgggtca
     1561 tggtgcgcac gacgcccagg cccactttgg cccgccccgg cgccgtgggg aagacccaga
     1621 catagccgcc cggcgcgtgg tcctggccga ccacgatttc cagcagccgg tcggtgggca
     1681 cgttggtcat ctcgtactgg atgcaggatg cggcttcgtg cagcggcagg gtggtgttga
     1741 tgcgcagcca cttggcgatg cgcgacccga cgccgtcggc ggcgaccagc acggtggcgc
     1801 ggatctcgaa cggcaggccg tggtggcgac cgcgcagcag ccagcccgcc gccgggtcgc
     1861 cggggtccac ccgcacggca tcctctaact cggtgcgcag cgccagttcg gcgcctgcct
     1921 ccgtcgccag cccggcgaca tgggtgtcca ggcgccgccg atccacgacc acgccgatgg
     1981 tctcgcgccc ggcgatctcg cggtagtcga tgcgccaggg ttcgccgtcg ttcgagtagg
     2041 tcaggaaccc ggcaatggag gagaccacga attcgtcgct gggcggcacc ccggcatcgg
     2101 tcagaccgcg caggctcacc gcgccggagc actccgtcgg gatgcccagt tcggcctttt
     2161 tgtccaggac cagcacgcgc aagccgcccg ccgccgcgta gcgcgcgcag ttggccccgg
     2221 cgggtccggc cccgactata accacatcat aatccgcgga tcgcagcatc tggtcccgat
     2281 tctagcccgt agggcaggtg gagaatgccc caattatccc accagggcgc gaaccttgtc
     2341 aagcacagca gggcccgatt gccgggggcc ggatggccct gaaagcgccg gccccacggc
     2401 ggcggaggtt tatgctacaa tagggcgtgc aacagggagt cgaaaggcgc gccagtgcgc
     2461 cgcacatgca tcgcgccatc gccggggcgc gcgtccggcg ggtcgtcggg ccgctgctgc
     2521 tctgcctcgt gggcgccctc atcctctgga cggcggcgtg gcaggtcaag cgcgccgaca
     2581 cggtcgacct cggcgggccg caggacggcc ccttcgtcca gcacttcaac acccgcgaac
     2641 ccgatcccgc caagcagccc aacccgccgt atacctaccg ctggagccag ggcaccagca
     2701 ccatcttctg gcccggcacc ggcaacttcc cgctgaccat caccctgcgc ctggcgagcg
     2761 tgcccctgcc cggcgcgccg cagaccgtca ccgccaccgt gcgcgggcag acctttccgc
     2821 tggcccttgc caacgagttg cgcgactaca gctttcgcgt cgagcgcggc aatcccctgg
     2881 aaggcgatgt ggccgtcacg ctgaccacga ccccgttcca cgggccgggc gacccgcgcg
     2941 aactcggggt gctggtggac agcgccacca tggcgcccat cgacaccggc gtgcgccccg
     3001 gcgtggtgcc gccgccgctg gatatggcgc gctgggccgc cgtgctggcg ctgctctacg
     3061 gcctgacctg gtggctgagc ggcagcacgc gggcggccac ggcgaccggg atcggcgtgg
     3121 ccctgttcgt ggcggtggaa gccatcggcg agcgggcgaa cctgggcttc ctggcgccgg
     3181 aagcctttgt gctgctgctg tgggcggtgc cgctggccgt cgtggcgcgc tgggccgcgc
     3241 gggccacgct gcgactggcg gcgcacgccg ccgggcaacc ggtccgcctg cccgcgcacg
     3301 gcgccgccga ggccgccgcc gccgcgctgg tcgcggggtg gctgctgcgc atcggcggca
     3361 tggtctaccc gcaattcatg agcagcgaca tcgggctgca cgtcaacaat atcggcaagg
     3421 tgctgcgcgg ccagtgggtc tgggccgggg tgctgcccaa cggcgcgccg cagccctacc
     3481 cgccgctgac ctacctgacg ctggcgccct tcgccgggct ggtgcccgat cttagcctgc
     3541 tgctgcgcgc cggggtgagc ctgctcgacg cggcctgcgt gctgccgctg ctctacctgg
     3601 gcacccggct gggcggggcg cgggcgggcg cctgggcggg ctgggtctac gcgctgctgc
     3661 ccgcgccctt cacgctcttc tcggcggggg tctacgccaa catcttcgcc aacatcgtct
     3721 tcacctggac gctggccctg tggggcgagc tgctgctggc gcgacgggtc acacccgcgc
     3781 tcgcggcagc cctggcggtg ggctttttct tgacattcat ggggcactat ggtatgctga
     3841 ttggcgctgt cgccgtcgcg gcggctttca ttgtgcttgc gttacttagc ggtccgccga
     3901 ttttacggcg gcgcgcactg atcgtcgcgg gcacgctggc cgtcgcgggc ctcgccgctt
     3961 acctgaccta ttaccagcac tttctggacg tgatgctggc ccaggtgcgt ggggtcggtg
     4021 cccaggtgac gccgggcggg gccacggatg ccgccgcggg cgacgtgacc ggcggcgccg
     4081 ccgcgatcac gccgggtgcc ctgctgaacc tgctgtggcg gcggctgggg ccggacctcg
     4141 gcgccgccgc gctgctggcg acgctggccg ggtggatcgc agccgggcgg gcggcacgcg
     4201 gtgtgcaggt gctggtcgtg gcgaccgcgc tggccgggat cggcttcgcc gccctgagcc
     4261 tgcgcgccgg cgagaatatt cgctacgcgt tgttatttgc gcccctggtc gcggtgggcg
     4321 cgggtctgtt tctgagccgc ctggatcgcc gggggccagc cggcctctgg tggagcgtcg
     4381 cgctggtcgc tgtgctggcc tggcacttgc tcgaagtctg ggtgccgctg attttcacgc
     4441 ggtaccacta gccaaggagc gacttgcact agcctatgat gcgccatgac ctctacgaag
     4501 acctgtacct gaaagaacag gactactggt ggcatgtcgg caagcgggcc atcgtctatt
     4561 ccctgctgga gaaattcctc ccccccggcg atccccagac gcggcacgcc ctcgacctcg
     4621 gctgcggcac cggccacaac ctcgacagcc tggcccgcta cgccgaaccg gtggggctcg
     4681 acagttccgc cgaggcgctg gccttctgcc ggcggcgcgg ccaccagcgg ctgatccagg
     4741 ccgcctcgct gcccttcgcc gacaactcgt tcgacatcgt caccgcgctc gacgtgatcg
     4801 aacacctgga cgacgacctg ggcaccctgc gcgaagtcta ccgggtgatg cgccccggcg
     4861 gcctgctgat catcagcgtc ccggcctaca aggtcctctg gagctactgg gaccagatcc
     4921 tgggccaccg ccgccgctac accacggcga acatggcccg cgtggtgcgc cgggccgggt
     4981 ggcgggtgcg caaggtcagc tacagcaacc tggcgatcct ggcgccgacg gtcgccgtgc
     5041 gcctggtcaa gagcctgcgc caccgcaaga gcgaggtcgc catgcccgcc agcgacagcg
     5101 ccgagcacat ggaaaccggg ggtccggaga ccgatttcgt cgacgtgccc ggctggttga
     5161 acagcgcgat gatcacctat taccgcggcg agagccgggc gttgcgggcc gtcgatctgc
     5221 cgttcggcct ctcggtcgtc tgcgtggcgc agaaaccgcg cgggagccgc ccgcagccgc
     5281 tatgagcaaa gtatccgcgg cgccggccct gcctcacgcg cggctggagg gcacccggtt
     5341 ggatgccgcg ttgcgcttct cggcctgggt ggagcggcag gcgccccggc tgctggtcgc
     5401 ggccatggcc gcctacgtgg tcatcctctt cgccgcctcg gtctacaagt acgagcattt
     5461 cggccagggc tacgaccagg tggacttcga gcaggcgctc tggaacaccg tccatggccg
     5521 ccccttcgcc gactcgcgct tcaacttcac cgactcgatc ttcggcatgg actggatgcc
     5581 gctgctggcc gtattcgtgc cggtctatgc cctgctgccg tcggcgctgc cgctgttctt
     5641 cctgcagatc ctggccgcgg ccctcggcgc gtggccggtc tacctgctgg cgcgcgacaa
     5701 gctgggcacc cgccgccacg ggctggtctt cggcctggcc tggctgctct accccacgat
     5761 ccagtattcg gtactcgatc ccttccagat ccgcatcttc gccaccacgc tgctgctgtg
     5821 cgccctgctc ttcttcgagc gcgcccggat gatccccttc ctgatcacgg cggggctggc
     5881 cctgctggcg cgcaccgatg tcgcgctggt cgtgctgatg ttcggcgtgt acgcgctgct
     5941 ggcgcggcgc ggcgtgcgct gggtgatcgc cccgctggcg ctgggcggcg gctatttcct
     6001 gctggtgatg acgttcatcg tgcccgcgtt cgtgcatctg cccgccgtcc actgcaccgg
     6061 gccgat
//
LOCUS       DASUYC010000790         4575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1510068, whole genome shotgun sequence.
ACCESSION   DASUYC010000790 DASUYC010000000
VERSION     DASUYC010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1510068"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VF276_12950"
     CDS             <1..399
                     /locus_tag="VF276_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HEX6607811.1"
                     /translation="IVAAPPEMSLLAVSVQFYGAPRIVLRVPPGAFHPPPKVESAVVR
                     VDVYPSDARPVAVADEAHFFAVARAGFGQKRKQLANSLADGLRLPKALVLDRLAQAGI
                     AGTRRAETLTLAEWATLSAALPATSEGETT"
     gene            402..1286
                     /gene="ispE"
                     /locus_tag="VF276_12955"
     CDS             402..1286
                     /gene="ispE"
                     /locus_tag="VF276_12955"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX6607812.1"
                     /translation="MTTTDSFELQAPAKINLTLEILGRRPDGYHNIASVMQAVSLYDT
                     LTFEHAPGGELVLDCADPALASQPQDNLVWRAARLLQQARGVTGGARITLEKGIPVAA
                     GLGGGSSDAAATLRGLDRLWNLGLQPRELQNLASLLGSDVTFFLHGGTALVEGRGEQV
                     TPLRPLTAGWFVLVTPPLATGDKTRLVYNLLRPYEHTNGVLTRRLVDLLAAGEVPPTT
                     MLYNGLEDAVFRAFDALDDVRRAVVAVGGESVRVTGAGPSLFVYCPTEDAARAIATAL
                     AADAVPVHVVQPVAGAAI"
     gene            1313..2194
                     /locus_tag="VF276_12960"
     CDS             1313..2194
                     /locus_tag="VF276_12960"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6607813.1"
                     /translation="MRIGPNDLVLDIGSGHSPHPRADVLCDRYIDDDTERGGSIRADR
                     PLIVADAHDLPFKDKAFDYVVTSHIIEHMDDPVRFCRELMRVARAGHIASPSEIAEKL
                     FYWPFHKWYVNKIGDTLVLHPKNDDPNIFGELFDYLYEYNPWYARFQRSLPDLFWIEY
                     EWEGEIKITIRQESPLDLDSPRRLRRMVKPRESLPTMLYRTAASIGEMVVPPAAIQGL
                     RALRRVKRSPARPIDLESILACPRCHSAVQIVGQEVRCTNPDCGKVYPIERGFPHLLL
                     DQALRERPPAPPLPRKR"
     gene            complement(2305..2601)
                     /locus_tag="VF276_12965"
     CDS             complement(2305..2601)
                     /locus_tag="VF276_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607814.1"
                     /translation="MTPDYTDYYQWEWSEVARHAATLHSGWELRGYITRRLDGWYAIA
                     DNPRDGLLHRYSLKGLPQAQGPYPTAEAAAQAARALIDSPANGSTEDPTTSVWT"
     gene            3045..>4575
                     /locus_tag="VF276_12970"
     CDS             3045..>4575
                     /locus_tag="VF276_12970"
                     /inference="COORDINATES: protein motif:HMM:NF013507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEX6607815.1"
                     /translation="MLSIIAGNVARRVEESSMRRFPWHVRGLVVFTALVLGGLFLVVH
                     PAQADRAPAVFVERTPLPAPVYQHAVAADGAYFYVIGGQSWNGSSVEALGEVYRYDPA
                     ANTYTARAVMPAQWQSLAACAMNGKIYVPGGYNVVGQSFDRTLYIYDIAGDHWTTGPT
                     LPAPGLIRYAAVCDAAANKLYVVSGSDISGTPWGVLKIFDAGANSWATGAPPPEGRAG
                     AQAGLINGKIYVAGGLPAGGTATADVQQYSIATDSWAAVAPMTAGGAFGAAGVYNGAL
                     YVAGGSTVSTNGGYLNRTERYDPAGNTWTTLDPLLRAVQFTGGAFLGNAFYVAGGRTT
                     YNTDPRCPGLASLGGICDYTQQLIVPPGASTATPTVTPTATRTRTPGATVTPPSATGT
                     ATGVPNVTATATSVPNATGTGTPAATATPVGTAVSSPTVPATATAAAASATPTGTAGP
                     EPTACALAFTDVPAGSAFYPYITCLACRGIVSGYQDGTFRPGNTLSRGQLAKVVAGGA
                     GF"
BASE COUNT          719 a         1535 c         1556 g          765 t
ORIGIN      
        1 atcgtggccg cgccgcccga aatgagcctg ctggccgtga gcgtgcagtt ctacggcgcg
       61 ccgcgcatcg tgctgcgggt gccgccgggc gcgttccacc cgccgccgaa agtcgagtcc
      121 gcggtggtgc gcgtcgatgt ctacccatcg gacgcccgac ccgtggcggt ggccgacgaa
      181 gcccactttt ttgccgtggc gcgcgccggg ttcggccaga agcgcaagca actggcgaac
      241 tcgctggccg acggcttgcg cctgcccaag gccctggtgc tggaccggct ggcccaggcc
      301 ggaatcgcgg gcacgcgccg tgcggagacg ctaaccctgg ccgaatgggc caccctgagc
      361 gcggcgctgc ccgctacctc cgagggagag accacctgac gatgaccacc accgattctt
      421 tcgagttaca ggcaccggcc aagatcaatc tcacgctcga aatcctgggc cgccgccccg
      481 acggctacca taatatcgcc agcgtgatgc aggcggtcag cctctatgac acgctgacct
      541 tcgagcacgc gccgggcggc gaactggtgc tcgactgcgc cgatcccgcg ctggcaagcc
      601 agccccagga caacctcgtc tggcgggcgg cgcggctgct acagcaggcg cgcggcgtga
      661 ccggcggagc gcgcatcacg ctggagaaag gcatccccgt ggctgccggg ctgggcggcg
      721 gcagcagcga cgcggcggcg accttgcgcg gcctcgaccg gctctggaac ctgggcttgc
      781 agccgcgcga gttgcagaac ctggcctcgc tgctcgggtc cgatgtgacg ttcttcctgc
      841 acggcggcac ggccctggtc gaagggcgcg gcgagcaggt gacgcccctg cgcccgctca
      901 cggcgggctg gttcgtgctg gtgacgccgc cgctggcgac cggcgataag acgcgcctgg
      961 tctacaacct gctacgcccg tatgagcata ccaacggcgt actgacccgc cggctggtcg
     1021 atctgctggc ggcgggcgag gtgccgccta ccaccatgct gtacaatggg ttggaagatg
     1081 ccgtgttccg ggcgttcgat gcgttggacg acgtgcgccg ggcggtggtc gccgtgggcg
     1141 gcgagtccgt ccgcgtgacc ggcgccggcc caagcctgtt tgtttattgt cccaccgaag
     1201 acgcggcgcg cgccattgcc acggcgctcg cggcagatgc ggtgcccgtc catgtggtgc
     1261 agcccgtggc cggggccgct atatagctgt agtttggaga ggtttttacc gtatgcgaat
     1321 tggtcccaac gatctggttc tcgacatcgg cagcggccat agtccgcacc cgcgggccga
     1381 tgtgctctgc gaccgctata tcgacgacga caccgagcgc ggcggctcga tccgggccga
     1441 ccgcccgctg atcgtcgccg atgcccacga cctgcctttc aaggataagg ctttcgatta
     1501 tgtcgtgacc tcacacatca tcgaacacat ggacgacccg gtgcgttttt gccgggagtt
     1561 aatgcgcgtg gcccgcgcgg ggcatatcgc ctcgccgtcg gagatcgccg agaagctgtt
     1621 ttactggcct ttccacaagt ggtatgtgaa taagatcggc gacaccctgg tgctgcaccc
     1681 caagaacgac gacccgaata tcttcggcga gttgttcgac tacctgtacg agtacaatcc
     1741 ctggtatgca cggttccagc gcagcctgcc cgatctcttc tggatcgagt acgagtggga
     1801 gggcgagatc aagatcacca tccgccagga gtcgccgctg gacctggata gcccgcgacg
     1861 gctgcggcgc atggtcaagc cccgcgagag cctgcccacc atgctctatc gcacggcggc
     1921 cagcatcggc gagatggtgg tgccgcccgc ggcgatccag ggactgcgcg cgctgcgccg
     1981 ggtgaagcgc agcccggccc ggcccatcga cctggagtcg atcctggcct gcccgcgctg
     2041 ccacagcgcc gtgcagatcg tgggccagga ggtgcgctgc accaaccccg actgcggcaa
     2101 ggtctacccc atcgagcgcg gcttcccgca cctgctgctg gaccaggcgc tgcgcgaacg
     2161 cccgcccgcg ccgccgctgc cgcgcaagcg ctagcggttg ccgccttgca ccgttttggc
     2221 ccttcccaaa actggggaag ggcctttgcg tgcgcgtggt ggcgttgcgc cggcggttgc
     2281 ggaggcacca ggcgcccggc agtattatgt ccagacggac gttgttggat cctcggtgct
     2341 tccgtttgcg ggcgagtcga tcagcgcgcg ggcggcctgc gcggcggcct cggcggtggg
     2401 gtagggaccc tgcgcctggg gcagcccttt gaggctgtag cggtgtagca gaccatcgcg
     2461 tggattatcg gcgatagcat accacccatc gaggcggcgg gtgatatagc cccgcaactc
     2521 ccacccgctg tgtagcgtgg cggcgtggcg tgcaacctcg ctccattccc actgatagta
     2581 atcggtataa tccggtgtca ttcgattcgg cttcaatcgg cgtgccggcg gcaacagcac
     2641 gggcgcgtac atctcctgat cccccgcaat attggggtca atcagagaca attgttgttt
     2701 ctactaacta aaacgattga acgtccgaaa ggtggcggta ttcggaggtt tgggggtaag
     2761 aatcgtggtc gccgccggct gcggcgggca gccagccgcc cgctatggtg cggccccggt
     2821 ccggtgtatg aggtgcgctc tgctgcccgc tgcgctggcc ttacatgaaa gcattgggca
     2881 aaacatatct gctggtaagg caattgttcg ttgacaccac acataccgca tgctagaatg
     2941 aggcccgttg cttgccggcg ggcgatctca tctgtagaga tggccgaagg caggtagact
     3001 ggacagggtt acctgcccgg ttgataaccg tacagcaccg gcgcatgctg tcgatcattg
     3061 cgggcaatgt agcccgccgc gtggaggaga gttcgatgcg gcgctttccc tggcatgttc
     3121 gcgggctggt ggtgttcacc gccctggtgc tcgggggcct gttcctggtc gtgcaccccg
     3181 cccaggcgga tcgcgcgccc gcggtgtttg tcgagcggac acccctgccc gcgccggtct
     3241 accagcacgc ggtggccgcc gacggcgcgt atttctacgt catcggcggg cagagctgga
     3301 acggcagctc ggtcgaggcg cttggcgagg tctaccgtta cgaccctgcg gccaatacct
     3361 acacagcacg cgcggtcatg cccgcccagt ggcagagcct ggcggcctgt gccatgaacg
     3421 gcaagattta cgtgccgggc gggtataatg tggtcgggca aagctttgac cggactctct
     3481 acatctatga tattgccggc gatcactgga ccaccggccc cacgctgccg gcgccgggcc
     3541 tgatccgcta tgccgcggtc tgcgatgccg ccgcgaacaa gctgtatgtc gtgagcggca
     3601 gcgacatttc gggcacgccc tggggggtgc tcaagatttt cgacgcgggc gccaatagct
     3661 gggcgaccgg cgcgccgccg ccggaggggc gggccggggc gcaggccggg ctgatcaacg
     3721 gcaagatcta tgtggcgggt gggttgccgg cgggcgggac ggccaccgcc gatgtgcagc
     3781 agtacagcat cgccaccgat agctgggcgg cggtggcgcc gatgacggcg ggcggcgcgt
     3841 ttggcgcggc gggcgtctac aatggcgcgc tctatgtcgc aggcggcagc acggtgagca
     3901 ccaacggcgg ctacctgaat cgcaccgaac gttatgaccc cgccggcaac acctggacga
     3961 cgctcgaccc gctgctgcga gcggtccaat tcaccggcgg cgcgttcctg ggtaatgcgt
     4021 tctatgtcgc gggcgggcgc accacgtaca atacggaccc gcgctgtccg ggcctggcct
     4081 cgttgggcgg catctgcgac tacacccagc agttgatcgt gccgccgggc gcatctacgg
     4141 ccacgcccac cgttacgccc accgcgaccc gcacccggac ccccggcgcc acggtgacgc
     4201 cgcccagcgc caccggcacg gcgaccggcg tacccaatgt cactgccacc gcgaccagcg
     4261 tacccaatgc caccggcacg gggacgcccg ccgcgacagc aacgccggta ggaactgccg
     4321 tgagcagccc cacggtcccg gccacggcta ccgccgctgc ggcgagcgcg acgcccaccg
     4381 gcaccgccgg gccggaaccg accgcgtgcg cgcttgcgtt caccgacgtg ccggcgggca
     4441 gcgcgttcta tccgtacatc acctgcctgg cttgccgggg catcgtcagc ggttaccagg
     4501 acggcacgtt ccgacccggc aacacgctca gccgcgggca actggcgaag gtggtggccg
     4561 ggggcgccgg gttca
//
LOCUS       DASUYC010000791         2392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_818413,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000791 DASUYC010000000
VERSION     DASUYC010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818413"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..713)
                     /locus_tag="VF276_12975"
     CDS             complement(123..713)
                     /locus_tag="VF276_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607816.1"
                     /translation="MPLGQRHRSSLFWIARGAIYLAATTAGGALFGALLGGIGAGLRP
                     LVPFGVLLVVAALLAAAYGLHEAGVWHLPHPERAWQVPNSWIMERPVLGAVAFGLILG
                     AGIFTFIPFTSFYLLLVWELLAGPVAGAVLGGAYGLARALPVLAGAWTSARGEPIAPV
                     HMRILTASPGLHRATSGLLLVSAGFLLVPLLLALAP"
     gene            complement(823..1347)
                     /locus_tag="VF276_12980"
     CDS             complement(823..1347)
                     /locus_tag="VF276_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607817.1"
                     /translation="MTDPSRLDPESQGTTAFDKMTRFASEELARAFDRRSFLKRAGGG
                     AFLFLAALASGQAFGGRTAGAQTPGQAGPRPTPWPSAPNAPCCSPPGPFCNLNGVNEP
                     NGCQGGNCYQHLSGGQVYNCTLYYTYYATGCWTTNCQGGYYTCCDCECFNGSGSRATC
                     GCAQFSGTPWRATD"
     gene            complement(1452..2018)
                     /locus_tag="VF276_12985"
     CDS             complement(1452..2018)
                     /locus_tag="VF276_12985"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HEX6607818.1"
                     /translation="MEGIWLVSFIALWIIVILEGVLIVLLYRQLGVLYLGSASGVSRD
                     GLAVGSAAPDFQLPDAEGRPHRLSDYRGRNVLLLFGSPHCDPCRRLLPNLEEFAATRD
                     FEVLWLNQALPEETRHFRAETGATLPMLSYQDKVNTDYHVRVTPFLFMIDPSGVIRAK
                     GLVNTKNQLDWYRDLFYGKATVGAAQGT"
     gene            complement(2039..>2392)
                     /locus_tag="VF276_12990"
     CDS             complement(2039..>2392)
                     /locus_tag="VF276_12990"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HEX6607819.1"
                     /translation="WAAFAAAALLVVFTVVVLGDLRGGRPMPCHCFGRFSQETAGPAT
                     VARNLVLLALATLLIAQPTPYLALDAVFGAAQGAVLPPLVNAVPVVFLALIAVVAVVL
                     GNSLLATIRSFLRAF"
BASE COUNT          430 a          784 c          875 g          303 t
ORIGIN      
        1 aacccggccc ctacagtggc agattacggc ggttgcctgg ttacaggccg gcaccgtcgt
       61 tttgcgttgt aggggccggt ttccaaccgg cctgccggcg cggggaaggg agaaccgggc
      121 gatcaggggg cgagcgcgag cagcagcggc accaggagga agcccgcgct cacgaggagg
      181 agaccgctcg tggcccggtg cagaccgggg ctggcggtca ggatgcgcat atggaccggg
      241 gcaatgggct cgccgcgggc cgaggtccag gcgccggcca gcaccggcag cgcccgcgcc
      301 aggccatagg ccccgcccag caccgcgccc gcgacagggc cggcgagcag ttcccagacc
      361 agcagcagat aaaacgaggt gaagggaatg aaggtgaaga tgcccgcgcc caggatcagg
      421 ccgaaggcca ccgcgccgag cacgggccgt tccatgatcc agctattggg cacctgccag
      481 gcgcgttccg ggtggggcag gtgccagacg ccggcctcat gtaagccata ggcggcggcc
      541 agcagggcgg cgaccacgag caagacgccg aagggcacga gggggcgcag gcccgcgccg
      601 atcccgccga gcagggcgcc gaacagcgcc ccgccggccg tcgtggccgc cagatagatc
      661 gcgccgcgcg cgatccagaa gagagaagag cggtgcctct gcccgagagg catgatcacg
      721 gagatcatgt tgacccctca ggcagaggag gtgcttgcca gcccggtgag gatcgccagg
      781 atgacgccca ccaccagcag gccgccggcc agtgtcagca tgtcagtcgg tggcgcgcca
      841 gggcgtgccg gagaactggg cgcagccgca ggtggcccgg ctgccgctgc cgttgaagca
      901 ctcgcagtcg cagcaggtgt aatagccgcc ctggcagttg gtcgtccagc aaccggtggc
      961 gtaataggta tagtacagcg tgcagttgta gacctgtccg cccgacaggt gctgatagca
     1021 gttgccaccc tggcagccgt tcggctcgtt cacgccgttg aggttgcaga agggaccggg
     1081 gggcgagcaa cacggcgcgt tgggcgccga cggccacggc gtggggcgcg gcccggcctg
     1141 ccccggcgtc tgcgcgcccg cggtccgccc gccgaacgcc tggccgctcg ccagcgcggc
     1201 gagaaacagg aacgcgccgc cgcccgcgcg cttgaggaag ctgcgccggt cgaacgcgcg
     1261 ggccagctcc tcgctggcga agcgggtcat cttatcgaaa gccgtcgtgc cctgggattc
     1321 cgggtccagc cgcgaggggt ccgtcatgag tactacacct ttcctgaact aatgaggcca
     1381 atggccggct aacctgacac acgagatgcc gagagagcgc gagtcgcgcg gacgcgggcg
     1441 ggcaaggcgg ctcaggtgcc ttgcgcggcg cccaccgtcg ctttgccgta gaacaggtcg
     1501 cggtaccagt cgagctggtt cttcgtgttc accaggccct tggcccggat cacgccgctg
     1561 ggatcgatca tgaacaggaa cggggtgaca cgcacgtggt aatcggtgtt caccttgtcc
     1621 tggtacgaga gcatgggcag ggtggcgccg gtctcggcgc gaaagtggcg ggtttcctcc
     1681 ggcagggcct ggttgagcca gagcacctcg aagtcgcggg tggccgcgaa ctcctccagg
     1741 ttgggcagca gccgccggca gggatcgcag tgcggcgagc cgaacagcag caagacgttg
     1801 cggccccgat agtcgctgag gcgatggggc cggccctcgg cgtcggggag ttggaagtcg
     1861 ggggcggcgc tgcccacggc caggccgtca cgcgagacgc ccgacgcgct gcccaggtag
     1921 agcaccccca gttgccggta gagcaaaacg attaagacgc cttccaggat gacgatgatc
     1981 cagagcgcga tgaagctgac gagccagatc ccttccatgt ggtccctttg gtcccctctc
     2041 agaatgcgcg caagaagcta cggatcgtcg ccagcaggct gttgcccagg accacggcga
     2101 cgacggcgat cagggcgaga aacacgaccg gcaccgcgtt caccagaggc ggcagcacgg
     2161 ctccctgcgc ggcgccgaac acagcgtcga gtgccaggta cggcgtgggc tgggcgatga
     2221 gcagcgtggc cagcgccagc aacacgaggt tgcgcgccac ggtggccggg ccggcggtct
     2281 cctgcgagaa gcgcccgaaa cagtggcatg gcatgggccg cccgccgcgc agatcgccca
     2341 gcacgaccac cgtgaacacg acgagcagcg ccgccgccgc gaacgcggcc ca
//
LOCUS       DASUYC010000792         2345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1511412, whole genome shotgun sequence.
ACCESSION   DASUYC010000792 DASUYC010000000
VERSION     DASUYC010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511412"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            363..>2345
                     /locus_tag="VF276_12995"
     CDS             363..>2345
                     /locus_tag="VF276_12995"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEX6607820.1"
                     /translation="MPAPTEVPAKAPAPPQAYSGALLRYPYLTDVTGPYATINWATVD
                     TSLNGYVRWGEAGVESCTAHTSPAGATVILVNRVREYQWQANLNLSPNTRYCYRVYLD
                     ATDLLGSDPSPIFWTQLPPHSDQAFSFAVFGDWGMVDANGQNPQQAAVIARIARSGAR
                     FAVTTGDNGYPSGSQNNYGDLVQVGANLSGVFGPAFWKVAGASMPIFPAMGNHGLDRA
                     DTTHPHLVNWPQLRTVTASGGVYARETYCCLNGTQPGDYPSAWYAFDAGGARFYVLDA
                     AWDDANLGTGTDYANDYAYHWQPSSEEYQWLARDLAAYPGTLKFAFLHYPFYSDDSTE
                     PSDPYLQGSANLEGLLARHGVAIAFSGHAHIYQRNLPVNGLVTYVTGGGGGDVVPVGG
                     RGCSSMDAYAIGWSYSNARGSACGSATPPTDDSQVHHYLLVTVNGTQVRVAPTDSLGH
                     TFDVRSYDFTPAPTPTPTPVGGNCGISFRDVSAGQTFAPYISWMACRGYISGYSCGGP
                     GDPCPGLYFHPNATVTRGQLLKMVVLAAGWPLVNPIVPSFEDTPATSPFYQYIETGAR
                     HGVISGYTCGGPGEPCTAPLGRPYFRPGASITRGQLSKVLALARGYSLPNPAQPTFAD
                     VPRSQPFFPYIEAMAAHNIVSGYTCGGSGEPCPGAYF"
BASE COUNT          389 a          854 c          728 g          374 t
ORIGIN      
        1 ggaacccgca gaccccaatc cagacccccc ggcgcgccgg ccgcagggcg cggctgacct
       61 gggcagccca ccgatccatg gtataatggg cagaatctaa tccgcgtcac gttagggcta
      121 aaatctcgtt cccttgccgg acggccccga ttcgggtatt ttgtggtcgg gcctgctacc
      181 gagaagcgct accgttggaa aggatccgca tgaaaacgat gggaccattg ggacgattag
      241 gcaccctact cttcgtactg ttcctgtgca tcagtgcggc ggggccatct gccgaggcgc
      301 aagccactgc cggtgtttca ggggcggcgc ctgcccctaa aggggcatct aaagaggcac
      361 ccgtgccggc gcccaccgag gtcccggcca aagcgccggc gccgccgcaa gcctatagtg
      421 gggcgctgct ccgctatccc tacctgacag acgtgacggg cccgtatgcc accatcaact
      481 gggccacggt cgatacctcc ctcaacggct atgtgcgctg gggtgaggcc ggcgtcgagt
      541 cctgcaccgc ccataccagc cccgccggcg ctaccgtcat cctggtcaat cgcgtccgcg
      601 aataccagtg gcaggccaac ctcaatctca gccccaacac ccgctactgc taccgcgtct
      661 atctcgacgc gaccgacctg ctggggtcgg atccctcgcc gatcttctgg acgcaactgc
      721 cgccccactc ggaccaggcg ttctcgttcg ccgtgttcgg cgactggggc atggtcgacg
      781 ccaacgggca gaatccccaa caggcggcgg tcatcgcccg gattgcccgc agcggcgccc
      841 gcttcgccgt taccaccggc gataacggct atccgagcgg cagccagaat aactacggcg
      901 acctcgtcca ggtcggcgcc aacctcagcg gcgtcttcgg ccccgccttc tggaaggtcg
      961 ccggggcgtc catgccgatc ttcccggcca tgggcaatca cgggctcgac cgcgccgata
     1021 ccacccatcc ccacctcgtc aactggcccc agctccgcac cgttaccgcg tccggcggcg
     1081 tctatgcccg cgaaacctat tgctgcctca acgggacgca gcccggcgac tacccgagcg
     1141 cctggtatgc cttcgatgcc ggcggcgccc gcttctacgt gctcgacgcc gcctgggacg
     1201 acgccaacct gggcaccggc accgactacg ccaacgacta cgcctatcac tggcagccga
     1261 gcagtgagga gtaccagtgg ctggcgcgcg acctggccgc ctatccgggc acgctcaagt
     1321 tcgccttcct gcactacccc ttctactccg atgattcgac ggaacccagc gacccctacc
     1381 tgcagggcag cgccaactta gagggcctgc tggcgcgcca cggggtggcg atcgccttca
     1441 gcggccatgc gcatatctac cagcgcaacc tgccggtcaa cggcctggtc acctatgtca
     1501 ccggcggcgg cggcggcgat gtcgtgcccg tcggcgggcg gggctgcagc tcaatggacg
     1561 cctacgcgat cggctggagc tacagcaacg cccgcggcag cgcctgcggc agcgcgaccc
     1621 cgccgaccga cgacagccag gtgcatcact acctgctggt gacggtgaac ggcacccagg
     1681 tgcgcgtcgc gcccaccgac tcgctaggtc acacctttga cgtgcgcagc tatgacttta
     1741 cccccgcccc cacgccgacc cccacccctg tcggcggcaa ctgcgggatc agcttccgcg
     1801 acgtgagcgc cgggcagacg ttcgcgccct atatttcgtg gatggcgtgc cggggctaca
     1861 tcagcggcta cagttgcggt gggccgggcg acccctgtcc ggggctctac ttccacccca
     1921 acgcgacggt gacgcggggg caactgttga agatggtggt gctggcggcg ggctggcccc
     1981 tggtcaaccc cattgtgccc agctttgaag atacgccggc caccagtccg ttctaccagt
     2041 acatcgaaac gggggcgcgg cacggggtca tcagcggcta cacgtgtggc gggccggggg
     2101 aaccgtgcac ggcgccgctg gggcggccgt acttccggcc gggggcgagc atcacgcggg
     2161 ggcagttgag caaagtgctg gcgctggcgc ggggctacag cttgcccaac ccggcccagc
     2221 cgaccttcgc ggatgtgccg cgcagccagc cgttctttcc gtatatcgag gccatggccg
     2281 cccacaacat cgtcagcggc tatacctgcg ggggcagcgg cgaaccctgc ccaggagcct
     2341 atttc
//
LOCUS       DASUYC010000793         3295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1775707, whole genome shotgun sequence.
ACCESSION   DASUYC010000793 DASUYC010000000
VERSION     DASUYC010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1775707"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..1633)
                     /locus_tag="VF276_13000"
     CDS             complement(143..1633)
                     /locus_tag="VF276_13000"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HEX6607821.1"
                     /translation="MTDTTLVVRAGAPIGTISPRLYGHFAEHLGRCCYDGLWVGTGPR
                     DIPQQAGFRSDVLAALRALPVPLLRWPGGCYADRYHWRDGIGPPAERPRRLGMSCGLQ
                     VEDDNSLGMHEFLHLCALLGAEPYLAGNVGSGTPQELCDWLEYCNSALDTTLTRLRAA
                     NGAPAPFRVPLWGVGNENWGCGGEYDAAGYAREYRRYASFLRPTDPAAELVACGYEDE
                     WNVELLRVIGNRLDLVDHLSIHHYWSHGGPETAFSADEYYALLAEAQSTEAFIARTAA
                     IIEAATGGRHRIGIALDEWGVWHPEARTWGPGCEIARSPVTYEQAGTLRDALAAAVAL
                     EGFHRQCRVLTLANLAQVVNVLHAPVMTDGPRMWLTPTYHVFRLHAPHLGATALPVEI
                     AHSGSLPDGTSGVTATASQSPAGLAVTVINRHLDQAASVSIRTDKFTGTAQGQVLTAG
                     TPSAGNSAEAPDRVAPVVLPVAADGPGAWRIDLPPHAVATIQVAAS"
     gene            2343..3134
                     /locus_tag="VF276_13005"
     CDS             2343..3134
                     /locus_tag="VF276_13005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607822.1"
                     /translation="MATQRQATARKLDRDPVSAPRDARNWRLDVTSQLDDSASGEALP
                     GFDAPARPRKPAPAAGPAERMTYAPPREGGVPFAQQRAAQMPQRAPARTPAIGTPLRL
                     AGLAVAGVAALLALYVVLSAVFGFLQVKMDDIQYGRPRTTHLDAYVGHAGEQDGQPSH
                     FLALNLDRRIVLIELPGGDTKNASTITGPYLFGDGEDLTPVKLSAEDVNGDGAADLLV
                     NVKNEQLVYINDKDKNTFHLMTPEERAKLPAEPTTGAPAARETSP"
BASE COUNT          591 a         1148 c         1045 g          511 t
ORIGIN      
        1 tctccattgg gcatcggatc attagctacg tatattcacc gcgaaggaac gaagaacgcg
       61 aagaacgagc ttaatagaag cgatcttcgc acgtctcatg tcttcgcatt aaggttacaa
      121 cggatgccga tccttcctaa ggctaactcg cggcgacttg gatcgtcgcc accgcgtggg
      181 gcggcagatc gatccgccac gcgccgggac cgtcggcggc gacgggaagg accaccggcg
      241 ccacccggtc cggtgcctcg gcgctgttgc ccgcgctcgg cgtgccggca gtcaggacct
      301 ggccctgcgc ggtcccggtg aacttatcgg tgcggatgct cacactcgcc gcctgatcca
      361 ggtgccggtt gatcaccgtc accgccagcc cggcggggct ctgcgaggcc gtggcggtta
      421 cgccgctcgt gccgtccggc aggctgccgc tgtgtgcgat ctcaacgggc agcgcggtgg
      481 cgcccaggtg cggcgcatgg agccggaaga catgataagt cggcgtcagc cacatgcgcg
      541 gcccgtcggt catcaccggc gcgtgcagca cgttcacgac ctgggccagg ttcgccaggg
      601 tcagcacgcg gcactggcga tggaagcctt caagggccac cgcggcggcc agcgcgtcgc
      661 gcagcgtgcc cgcctgctca taggtgacgg gcgaccgggc gatctcgcag ccgggccccc
      721 aggtgcgcgc ctccgggtgc cacacgcccc actcgtccag cgcgatgccg atgcggtgcc
      781 gcccgcccgt ggccgcctcg atgatggccg ccgtgcgggc gataaaggct tcggtgctct
      841 gtgcttcggc cagcagcgcg taatattcgt cggcgctgaa ggcggtttcg gggccaccgt
      901 gcgaccagta gtggtggatg gagaggtgat ctaccagatc gagccggttg ccgatcacgc
      961 gcagcaactc gacgttccac tcgtcttcgt agccgcaggc gaccagttcc gccgccgggt
     1021 cggtcgggcg caggaacgag gcatagcggc ggtattcgcg ggcatagccg gcggcgtcgt
     1081 actcaccgcc acagccccag ttctcgttgc ccacgcccca cagcggcacg cggaagggcg
     1141 cgggcgcgcc gttcgccgcg cgcagccggg tcagcgtggt gtccagggca ctgttgcagt
     1201 attccagcca gtcgcacagc tcttgcggcg tgccgctgcc cacgttgccc gccaggtagg
     1261 gttcggcgcc cagcagcgcg cagaggtgca agaactcatg catccccagg ctgttgtcgt
     1321 cctcgacctg caagccgcac gacatcccca gccggcgggg ccgctcggcc ggcggaccga
     1381 tgccgtcgcg ccagtggtag cggtcggcgt agcagccgcc cggccagcgc aagagtggca
     1441 ccggcagcgc gcgcaacgcc gccagcacat cgctgcggaa ccccgcctgc tgcgggatgt
     1501 ccctgggccc ggtacccacc cacaggccgt cgtagcagca gcggcccagg tgctcggcga
     1561 aatgcccgta gagccggggc gagatcgtgc cgataggcgc gcccgcgcgc accaccagcg
     1621 tggtatccgt catgggttgc tcctttagcg ctgcttccag tcgcctttgt cactctgagc
     1681 gcagcgaaga gtctcgtctc gtcgcctttg tcactctgag cgcagcgaag agtcccgtct
     1741 cgttggggaa cagacgagat gcttcactgc gttcagcatg acaactagca ctggaagcac
     1801 tattccccgt tcgcgcaaca aatcgccgaa agggcaggct atgcagcatt gcgcctgatg
     1861 ccggacagct actagccaca gcagggattg taacgggttg gatgcaacgg cgtgactgcc
     1921 gggcaggccg ctatgacgcc ggcccccgct aatacgcctg gctagtggtc tatcacaatg
     1981 atcctgatcg gcagccatgg ccggttttaa accggcccct acaacgcaaa atgacggcag
     2041 ctaaccacta cgcaccgctt gtcccaccaa tgccgccacg cggtgccacg ccggtgccgc
     2101 cgcccaccgt ggtaccaccc ggaagcggca aatttcagcc gtaaaatcga ccctgagcag
     2161 gcaccggtaa ggtcttgaca ggcacgaaca ggtgttctat aattgcttca tggtcgttga
     2221 acacgtgttc tgatcctttt ggttcccggc cggaccaccc gctacgcacc tattttaaag
     2281 accgaaccag ttcaaatcaa gacccgagaa aagacgctta ttaggagaaa gcgaggatct
     2341 ccatggctac tcaacggcaa gccaccgccc gcaagctgga ccgcgacccg gtaagcgcgc
     2401 cccgcgacgc ccgcaattgg cggctggatg tcaccagcca gttggacgac agcgcatccg
     2461 gcgaggcgct gcccggattc gacgcgcctg cccgcccgcg caaacctgcg cccgctgccg
     2521 gcccggctga gcgcatgacc tatgcgccgc cgcgcgaggg gggcgtgccc ttcgcccagc
     2581 agcgcgccgc gcagatgccc cagcgcgcac ccgcccgcac gcccgctatc ggcacccccc
     2641 tgcgcctggc ggggctggcg gtcgccgggg tcgcggcgtt gctggcgctc tacgtggtgc
     2701 tcagcgccgt attcggcttc ctgcaggtga agatggacga catccagtac ggccgcccgc
     2761 gcaccaccca cctcgacgcc tatgtcggtc atgcgggcga gcaggacggg cagcccagcc
     2821 acttcctggc gctgaacctg gaccgccgca tcgtgctgat cgagctgccc ggcggcgaca
     2881 ccaagaacgc cagcaccatc accggcccct acctcttcgg cgacggcgag gacctgacgc
     2941 cggtcaagct gagcgcggaa gacgtgaacg gcgacggcgc cgccgatctg ctggtgaacg
     3001 tgaagaacga gcaactggtc tacatcaacg ataaggacaa gaataccttc cacctgatga
     3061 cgccggaaga gcgggccaag ctgcccgcgg aacccaccac gggcgcaccc gccgccagag
     3121 agaccagccc atgagcagca atgctccgcc gttaaacgca tacgcctaca accgggacga
     3181 gtacagtcgc gacgcaaaga gcttcggctt tagccgacca tccccgcgtt ccagtgccta
     3241 tctggaggcg ctcgacctgg aacacggtgc gtacacctat acgacaccgc ccagc
//
LOCUS       DASUYC010000794         3413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1278466, whole genome shotgun sequence.
ACCESSION   DASUYC010000794 DASUYC010000000
VERSION     DASUYC010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278466"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="VF276_13010"
     CDS             <1..587
                     /locus_tag="VF276_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004419697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HEX6607823.1"
                     /translation="MDVQLAGELNGITAAVALRREYPRLPVVFYSIQDDDAYYRAFRR
                     SGILSHYAYVRKSNYLLPQMIVPLLHDARAGRGFIDPEIESRVQEVRHKDAQAPMALL
                     EPNEQAVARMLAQGMTNEQIAARMGFRDKRTISRTNGQIYNAWGLNDSTTDEKVARTR
                     AAVIARAGRLITWDADGTPRVLNERGEWVVWEGS"
     gene            563..2716
                     /locus_tag="VF276_13015"
     CDS             563..2716
                     /locus_tag="VF276_13015"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6607824.1"
                     /translation="MGRLGGIMIGEPFLIWAGIAVSLFNTILLCWLGLTVLLNAERRT
                     WGIWLAGGGLLMGAAFFISHAALLAYGFNVVNWEVNFWWQVAWVPVIVLPFAWYVVML
                     WYAGFWDDRQTPLHRRQRPWLLSILVLTLGMLGVLLWVNLFPLYGVLIHQFAAGVLPI
                     GNIPLRVLAYPAYILLCIILSLDALRRPGPTGRVMGDQARRLARPWLVATSALLLLVS
                     LLVTGVLLWIVLNTATLYIVYSEMAVVIAASDVVICLLISLAVVLLGQAIVSYEIFTG
                     KALPRRGLARHWHSAVLVAAGYSLVVAGTATLPIRPIYSLLLATVLMTLFYALFSWRS
                     YAERERYMDQLRPFVASQRLYESLLTRATPPAIDATTPFHALCADVLGARRAYLVPLG
                     PLSSLAGPMLAYPPGEDAVLPAAAGLAAQFPSPDTMYAPVDPARYGGALWAVPLWSER
                     GLIGVLLLGEKSAGGVYTQEEIEIARASGERLIDTQASAEIAGRLMVLQRRRLAESQV
                     LDRRARRALHDDVLPRLHTAILNLSSGPQAGETLPDTLRLLADVHRQISNLLREMPTG
                     SAPEVTHLGLIGALQQTLRDELGAAFDAVTWEIAPEAAQAAEAMPALTAEVLFYAARE
                     AIRNAARHGRAADLARPLRLRIAVEWRDGLEILIEDDGVGLGAPPAPNGGSGQGLALH
                     GTMMAVVGGALAAESVPGRYTRVLLTLPQTAAWAA"
     gene            complement(2713..3204)
                     /locus_tag="VF276_13020"
     CDS             complement(2713..3204)
                     /locus_tag="VF276_13020"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6607825.1"
                     /translation="MPHGPGKKALIIVEDNAAVAEVIKDTLNGEPGYRAVVVHDGAQA
                     LEVIHSIQVDAVLLDVALPGLSGPQIYEALKADNATRHLPVIFITAHYDSPDFQRLGI
                     TRFIAKPFNLDELLARVEEVCGPGLPVEPVPAPAEDHTLLDASLHDDGDILYFLTVPQ
                     PEL"
BASE COUNT          481 a         1280 c         1095 g          557 t
ORIGIN      
        1 tgatggatgt gcagttggcc ggggagttga acgggatcac ggcggcggtg gccctgcgcc
       61 gcgagtatcc ccgcctgccg gtcgtctttt actcgatcca ggacgacgac gcctattacc
      121 gcgcatttcg tcgctccggc atcctcagcc actacgccta tgtccgcaag tccaactacc
      181 tactgccgca gatgatcgtg cccctgctgc acgacgcccg cgccgggcgc ggcttcatcg
      241 acccggagat cgagtcgcgc gtgcaagagg tgcggcacaa ggacgcgcag gcgcccatgg
      301 ccttgctgga gccgaacgag caggccgtgg cccgtatgct ggcccagggc atgaccaacg
      361 agcagatcgc ggcgcgcatg ggcttccgcg acaagcgcac catcagccgc accaacggcc
      421 agatctacaa tgcctggggc ctcaacgaca gcaccaccga cgagaaggtg gcgcgcaccc
      481 gcgccgcagt gatcgcgcgc gccgggcggc tcatcacctg ggacgccgac ggcacgccgc
      541 gcgtgctcaa cgagcgcggc gagtgggtcg tctgggaggg atcatgatcg gcgaaccctt
      601 cctcatctgg gccgggatcg ccgtctcgct gttcaacacc atcctgttgt gctggctcgg
      661 cctcaccgtg ctgctgaacg ccgagcggcg cacctggggc atctggctgg cgggcggcgg
      721 cctgctcatg ggcgcggcct tcttcatcag ccatgccgcc ctgctggcct acggcttcaa
      781 cgtggtcaac tgggaggtga atttctggtg gcaggtggcc tgggtgccgg tcatcgtgct
      841 gccctttgcc tggtacgtgg tcatgctctg gtacgccggc ttctgggacg accggcaaac
      901 gccgctacat cggcgccagc ggccctggtt gctcagcatc ctggtgctca cgctcggcat
      961 gctcggcgtg ctgctctggg tcaacctgtt ccccctctac ggcgtgctca tccaccagtt
     1021 cgcggcgggc gtgctgccca tcggcaatat ccctctgcgc gtgctggcct accccgccta
     1081 catcctgctc tgcatcatcc tgtcactcga cgcgctgcgc cggcccggcc ccaccgggcg
     1141 cgtcatgggc gaccaggcgc ggcggctggc ccggccctgg ctcgtcgcca cctcggcatt
     1201 actgctgctg gtcagcctgc tggtgacggg cgtgctgctc tggattgtgc tcaacacagc
     1261 cacgctctac atcgtctaca gcgagatggc cgtggttatc gccgccagcg acgtggtgat
     1321 ctgcctgctc atctcgctgg ccgtcgtgct gctcggccag gccatcgtct cctacgagat
     1381 cttcaccggc aaggcgctgc cgcgccgggg cctggcccgc cactggcaca gcgccgtgct
     1441 cgtcgccgcg ggctacagcc ttgtcgtcgc cgggacggcc accctgccca tccgccccat
     1501 ttacagcctg ctgctggcaa cggtgctgat gacgctgttc tatgccctgt ttagctggcg
     1561 atcctacgcc gagcgcgagc gctatatgga ccagttacgc cccttcgtcg ccagccagcg
     1621 gctctacgaa tcgctgctca cccgcgccac gccgccggcc atcgacgcga ccacgccctt
     1681 ccacgccctc tgtgccgacg tgctgggcgc gcgccgcgcc tatctggtgc ctctgggacc
     1741 gctctcctcg ctggccggcc ccatgctggc ctacccgccg ggcgaagacg ccgtgctgcc
     1801 cgccgccgcg ggcctggccg cgcagtttcc cagccccgac accatgtacg ccccggtgga
     1861 cccggcgcgc tacggcggcg ccctctgggc cgtgccgctc tggagcgagc gcggcctgat
     1921 cggcgtgctg ctgctgggcg agaagagcgc cgggggcgtc tacacgcagg aggagatcga
     1981 gatcgcgcgg gccagcggcg agcgcctcat cgacacccag gccagcgccg agatcgccgg
     2041 gcggctgatg gtcttgcagc gccgccgcct ggccgaaagc caggtgctcg accgccgcgc
     2101 acgccgcgca ctgcacgacg acgtgttgcc gcgcctgcac acggccatcc tcaacctgag
     2161 cagcggcccg caggccggcg agaccctgcc cgacactctg cgcctcctgg ccgatgtcca
     2221 ccgccagatc tcgaacctgc tgcgcgagat gcccaccggg agcgcgcccg aagtgaccca
     2281 tctgggcctc atcggtgcgc tgcaacagac gctgcgcgat gaactgggcg ccgccttcga
     2341 cgccgtcacc tgggagatcg ccccggaggc cgcgcaggcc gccgaagcca tgcccgcgct
     2401 caccgccgag gtgctgttct atgccgcccg cgaggccatc cgcaacgccg cccgccacgg
     2461 gcgcgccgcc gacctggccc gcccgctgcg cctgcgcatc gccgtggagt ggcgcgacgg
     2521 gttggagatc ctcattgagg acgacggggt gggcctgggc gccccgcccg cgccgaacgg
     2581 cggcagcggc cagggcctgg ccctgcacgg caccatgatg gccgtcgtcg gtggtgccct
     2641 ggccgccgaa agcgtgcccg gtcgctacac ccgcgtcctg ctcaccctgc cccagaccgc
     2701 cgcctgggcc gcctaaagct ccggctgcgg caccgtcagg aaatagagga tatccccgtc
     2761 gtcgtgcagg ctggcgtcga gcagcgtgtg gtcctccgcc ggcgccggca ccggttccac
     2821 cggcagcccc ggcccgcaca cctcctcgac ccgcgccagc agttcgtcga ggttgaacgg
     2881 cttggcgata aaccgggtaa tccccaggcg ctggaagtcg ggcgagtcgt agtgggcggt
     2941 aatgaagatg accggcaggt gccgggtcgc gttgtcggcc ttaagcgcct cgtagatctg
     3001 cgggccgctc agccccggta gcgccacgtc aagcagcacc gcatccacct ggatcgaatg
     3061 gatgacctcc agcgcctgcg ccccgtcatg tacgaccacg gcgcgatacc ccggctcccc
     3121 gttgagcgtg tccttgatca cctccgccac cgccgcgtta tcctcgacga taatcagcgc
     3181 tttcttcccc ggcccgtgcg gcatggctac tcccctttcc ctgaccttcc acgccccgca
     3241 agtcgtatcc tagcaagaca tatgccgggt agatcaacat tacgcacact gacggcgcaa
     3301 atccgccccg ccgcggaccg gaaaggcgcc tggcgtggct ccgccgggga gtcacgccag
     3361 gccggcgcgc gcggcgacgt gcccgcgcta cgctttacgg tccatatttc gcc
//
LOCUS       DASUYC010000795         2144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_819225,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000795 DASUYC010000000
VERSION     DASUYC010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819225"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VF276_13025"
     CDS             complement(<1..282)
                     /locus_tag="VF276_13025"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX6607826.1"
                     /translation="MSNLYESGRAAAPDADSAAPAVPYTIGLAQINPTLGDVERNLAK
                     HYEYIEQAATRGVDLLVFPEASLTGYYLQDLMDDVAMRCDALPLLSLAGA"
     gene            complement(424..1629)
                     /locus_tag="VF276_13030"
     CDS             complement(424..1629)
                     /locus_tag="VF276_13030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6607827.1"
                     /translation="MKRHRSAKAPAPWLVGYTALVAGAAGVNVGMSYRQPPHPAIKHA
                     AGPDADPPPPGLDAWPFVSIIVPCRNEERNLPTLLPSLLGQSYPADRYEVIVVDDQST
                     DRTPAILAEFAADPQLRVVAGQPLPAGWKGKPWAMHQGAAVARGEWLLFTDADTMHEP
                     VALASAIYDAVERGADLFTFAPCMVLASPGERLIMPVVVLGISTFYHPVFVNNPRNPT
                     AIANGQYILVRRAVYDAVGGIDTVRDAIGEDLEFGKLIKRSGYRLWLTNGQDLMRVRM
                     YQNFVEVWEGWRKNVLLSMRKQPVMGVMQLGSLFIGGILPFALFARYLLAALRRGAAG
                     PDLALSGIQVAALIAMKRRVDQLLELPVGWAVTFPVGIFLFTLILLDSLRRLISGEGV
                     TWKGRAYTR"
     gene            complement(1746..>2144)
                     /locus_tag="VF276_13035"
     CDS             complement(1746..>2144)
                     /locus_tag="VF276_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607828.1"
                     /translation="TSAPTTGPITSPPLRDITPVAPPSGVGAVITPDAGGVAAVNVDL
                     TGATVQLQTGDTLQVNLTGNYDWSVTLANPAVLSVVSAPPATGAGRAVYKAAQAGQTT
                     LLLAGDPPCLKSNPPCGLMSRAVEFTIIVR"
BASE COUNT          335 a          722 c          747 g          340 t
ORIGIN      
        1 ggcgccggcc aggctcagca gcggcagcgc gtcgcagcgc atcgccacgt cgtccatcag
       61 gtcttgcagg taatagccgg tcaggctcgc ttcggggaag acgagcagat cgacgccgcg
      121 cgtggcggct tgctcgatat actcatagtg cttcgccagg ttgcgctcga catcgcccag
      181 ggtcgggttg atctgcgcca ggccgatggt gtatggcacc gccggcgcgg cgctgtccgc
      241 gtcgggcgcg gccgctcgcc cggattcata cagattgctc atactacctc ccgctatccg
      301 gccgccggat tagtcgtcca atatggggtt cggataggcg gccaggccgg tttgaaaccg
      361 gcccctacac cgagacatta cggtggccgc cggcgcccac ggtggcgggg cgggccctgc
      421 ctgttaccga gtgtacgcgc ggcctttcca ggtcacgcct tcgccgctga tcagccggcg
      481 caggctgtcg agcaggatca aggtgaagag gaagatgccc accgggaagg tgacggccca
      541 gccgaccggc aactccagca gttgatcgac gcgccgcttc atggcgatca gcgcggcgac
      601 ctggatgccg ctgagcgcga gatccggccc ggcggcgccc cggcgcagcg cagccagcag
      661 atagcgggcg aacagggcaa agggcaagat gccgccgatg aacaggctgc ccagttgcat
      721 cacgcccatc accggctgct tgcgcatcga cagcagcaca ttcttgcgcc agccctccca
      781 gacctccacg aaattctggt acatgcgcac gcgcatcaga tcctgcccgt tggtcagcca
      841 gagccggtag ccgctgcgct taatcagctt gccgaactcc aggtcctcgc cgatggcgtc
      901 gcgcacggtg tcgataccgc ccaccgcgtc gtagaccgcc cggcgcacga ggatatactg
      961 gccgttggcg atggcggtgg ggttgcgcgg gttgtttacg aagaccgggt ggtagaacgt
     1021 cgagatgccc aggacgacga cgggcatgat caggcgttcg ccggggctcg ccagcaccat
     1081 gcatggcgcg aaggtgaaca ggtcggcgcc gcgctcgacg gcgtcgtaga tggccgaggc
     1141 cagggccacc ggctcatgca tcgtatcggc gtcggtgaac agcagccact ccccgcgggc
     1201 gacggcggcg ccctggtgca tcgcccaggg cttgcccttc cagcccgccg gcagcggttg
     1261 cccggcgacc acgcgcagtt gggggtcggc ggcgaactcg gccaggatgg ccggtgtgcg
     1321 atccgtcgat tggtcgtcca cgacgatgac ctcgtagcgg tccgccgggt aggactggcc
     1381 gagcaggctg ggcagcagcg tgggcaaatt gcgctcttcg ttgcggcagg gcacgatgat
     1441 cgacacaaac ggccaggcgt ccagacccgg cggaggcgga tcggcgtccg gcccggcggc
     1501 atgtttgatc gcggggtgcg gcggctggcg gtaggacatg cccacgttca cgcccgccgc
     1561 gccggccacc agggccgtgt agcccaccag ccagggcgcg ggcgccttcg ccgatctatg
     1621 acgcttcact agccatcctt cctcggttct tgtcgctctg atgttattgt cgcgctgcga
     1681 ccattgttac tctaggcgca ccgcagagtc ccgccgcgtg cgccgacaag cacgtctgcc
     1741 ggcaactagc gcacgatgat cgtgaactcg acggcgcgcg acatcagccc gcagggcggg
     1801 ttcgacttga ggcacggcgg atcgccggcg agcagcaggg tggtttgccc ggcctgggcg
     1861 gctttgtaga ccgcgcgccc cgcgccggta gccggcgggg cactcacgac gctgagcacc
     1921 gccgggttcg ccagggtgac actccagtcg taattcccgg tcaggttcac ctgcaacgtg
     1981 tcgcccgttt gcagttgcac ggtggcgccg gtcaggtcca cgttcaccgc cgccacgccc
     2041 ccggcatcgg gcgtgatcac ggcgcccacc ccgctcggcg gcgcgaccgg cgtaatatcg
     2101 cgcaggggcg ggctggtgat cgggccggtc gtcggggccg aggt
//
LOCUS       DASUYC010000796         2380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_93461,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000796 DASUYC010000000
VERSION     DASUYC010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93461"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1629)
                     /locus_tag="VF276_13040"
     CDS             complement(<1..1629)
                     /locus_tag="VF276_13040"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEX6607829.1"
                     /translation="MSKHPESRGNAPAPGSSVELALADMAYGGDAVGRSDGAVVFAWG
                     GISGEQVTARVDRVKRDIAWATVTHVETPSPHRVAPPCPYFGPCGGCQWQHIDYGGQL
                     DFKTAILRDQLRRFGGLDPAHLDQVLQPAVGMSEPWHYRNVAHFQIDPSTRALGYFRR
                     NSHDVVPIAVCPISDPGINQLLTSVQALFDTHAQAPADLPPNLSDVDLESVRRGQMPV
                     AVRERKVTGMPVWQVTIRTGAPPAEPVEAETPWAGREMVVILHTLSGDPPPARRGRGR
                     GAPEEAAPRTPVLIPRKAVRQWVAGLGGRVSVVELRQDGALELVAETPEAAALSGEEL
                     ADQPMPGRVSGSRRSARQEAAEDRPEAPPGVVRQRLGDGRYWVAPHAFFQVNNIQAEK
                     ILDLIRAALPEPVDLLVDAYSGVGAFALALLAGGQARRVVAIESDWAAVESARWTAAL
                     RQIPAEQLQLEPGRVEEVLPALDVEPDVVLLDPPRTGCAPALLQYLLAHPPARLIYVS
                     CDPSTLARDLRALAPGYTLQRIQAVDMFPQTYHIE"
     gene            complement(1626..1913)
                     /locus_tag="VF276_13045"
     CDS             complement(1626..1913)
                     /locus_tag="VF276_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEX6607830.1"
                     /translation="MKLVLGIVQKADTDAVLDALTEGGYRATRLASTGGFRREGNTTL
                     IMGVDDHQVDPLIRLLRDTVARRAERSSTHPDGSTGRGAVFVLPLTDSFHL"
     gene            complement(2012..>2380)
                     /locus_tag="VF276_13050"
     CDS             complement(2012..>2380)
                     /locus_tag="VF276_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEX6607831.1"
                     /translation="ILIAASGWRLMRNTVRILLESVPSEIRTEEVVAAMRAVPGVQDV
                     HDLHIWQIASGMYALSCHVLVDDQMLSQGSSIITTLNTLLADRFHIGHTTIQPECLNC
                     DPNSLFCALAPTPVAHDHQH"
BASE COUNT          332 a          827 c          852 g          369 t
ORIGIN      
        1 ttcgatgtgg tacgtctgag ggaacatatc cactgcttgt atgcgctgga gcgtgtagcc
       61 gggggccagg gcgcgcagat cgcgagcgag ggtgctggga tcgcaggaga cgtagatcag
      121 gcgcgcgggc gggtgggcca gcaggtactg cagcagggcg ggcgcgcagc cggtgcgcgg
      181 cgggtccagc agcacgacat cgggctccac gtcgagcgcg ggcagcacct cttccacgcg
      241 gcccggctcc agttgcagtt gctcggcggg gatctgccgc agcgccgccg tccagcgcgc
      301 actctcgacg gcggcccagt cgctctcaat cgccaccacc cggcgcgcct ggccgcccgc
      361 cagcagggcc agcgcgaagg cgcccacgcc gctgtaggca tcgaccagca ggtccaccgg
      421 ctcgggcagc gcggcccgga tcaggtccag gatcttttcg gcctggatgt tgttgacctg
      481 gaagaaggcg tgcggcgcga cccagtagcg cccgtcgccc aggcgctggc gcaccacgcc
      541 gggcggggcc tcgggccggt cctccgccgc ctcctggcgg gcgctgcgcc ggctgccgct
      601 gacccggccc ggcatgggct ggtcggccag ttcctcgccg ctaagggccg ccgcctccgg
      661 cgtttcggcc accagttcca gcgcgccgtc ctggcgcagt tcgaccacgc tcacgcgccc
      721 gcccagcccg gccacccatt ggcgcaccgc tttgcgcggg atcaagaccg gcgtgcgcgg
      781 cgccgcttct tcgggcgcgc cccggccccg cccgcgccgc gccgggggcg ggtcgccgct
      841 cagcgtgtgc aggatcacca ccatttcgcg cccggcccag ggcgtctccg cctcgaccgg
      901 ctcggcgggc ggcgcgccgg tgcgaatcgt cacctgccac acgggcatgc cggtcacttt
      961 gcgttcgcgc actgccaccg gcatttgccc ccggcgcacc gactccaggt ccacatcgct
     1021 gaggttgggc ggcaaatcgg cgggggcctg ggcgtgggtg tcaaacagcg cctgcacgct
     1081 ggtcagcaac tggttgatgc ccggatcgct gatcgggcag acggcgatgg gcaccacgtc
     1141 gtggctgttg cggcggaaat agccgagtgc gcgggtggac gggtcgatct ggaagtgggc
     1201 cacgttgcgg tagtgccagg gttcgctcat gcccaccgcc ggttgcagca cctggtccag
     1261 atgggccggg tccaggccgc cgaaccggcg caactggtcg cgcaggatgg ccgtcttgaa
     1321 atccaactgc ccgccgtagt cgatgtgctg ccactggcag ccgccgcagg ggccgaagta
     1381 ggggcagggc ggcgccacgc ggtgcgggga aggagtctcg acatgggtga cggtggccca
     1441 ggcgatgtcg cgcttgaccc ggtccacgcg ggccgtgacc tgttcgccgc tgatgccgcc
     1501 ccaggcgaag accaccgcgc cgtcgctgcg gcccaccgcg tcgccgccgt aggccatatc
     1561 ggccagggcc aactcaacgc tgctgccggg cgccggggcg ttgccgcgcg actcggggtg
     1621 cttgctcata ggtggaagga gtcggtcaag ggcagcacga acacggcgcc ccggcccgtc
     1681 gagccgtcgg gatgggtgga ggagcgctcg gcccgccgcg ccaccgtgtc ccgcagcagc
     1741 cggatgagcg ggtccacctg gtgatcatcg acgcccatga tcaaggtggt gttgccttcg
     1801 cggcggaagc cgccggtgct ggcgaggcgc gtcgcccggt agcccccctc ggtcagcgcg
     1861 tccagcacgg cgtcagtatc cgctttctgc acaatcccca gtactaattt catgcctgct
     1921 ctcccccgaa ctgctctccg gcgtcagggc cgggtgcctg cattatagcg atattcccgc
     1981 acccggtcaa tggttgggtg tccgagtggt atcaatgctg gtgatcgtgc gcgaccggtg
     2041 tcggggccag cgcgcagaag agcgagttgg ggtcgcagtt caggcattcc ggctggatcg
     2101 tcgtgtgccc gatatggaag cggtcggcca gcagcgtgtt cagcgtggtg atgatcgacg
     2161 atccctggct tagcatctgg tcgtccacca gcacgtggca gctcagcgcg tacatgcccg
     2221 acgcgatctg ccagatatgc aggtcatgga catcctgcac gccgggcacg gcccgcatgg
     2281 ccgcgaccac ctcttccgtg cggatctccg acggcaccga ttcgagcaag atgcgcacgg
     2341 tgttgcgcat gagccgccag ccgctggccg cgatcaggat
//
LOCUS       DASUYC010000797         2416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1049556, whole genome shotgun sequence.
ACCESSION   DASUYC010000797 DASUYC010000000
VERSION     DASUYC010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049556"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(392..763)
                     /locus_tag="VF276_13055"
     CDS             complement(392..763)
                     /locus_tag="VF276_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607832.1"
                     /translation="MSQPNQYVLHGEHTQITYSSTGFAGKPQLHYQDRQHNVTAGGPD
                     IRTEETEIGTQVTIVVEVTAIHNRRLTLLIPAINLRGEETEAALQTVAILTTTQTETV
                     APVRQSYHALTLRGTARQVEF"
     gene            953..1207
                     /locus_tag="VF276_13060"
     CDS             953..1207
                     /locus_tag="VF276_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607833.1"
                     /translation="MAKEVILTLADDTYAALAAWAGGADQVAPYLQRLVEAILAHPAQ
                     EEQPVRRGRAPGLLAMPADADDDDPFHLLAEDQRWGGPGG"
     gene            1332..1556
                     /locus_tag="VF276_13065"
     CDS             1332..1556
                     /locus_tag="VF276_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607834.1"
                     /translation="MNLQQAMLAAQLAHARATAAQDLIDDMDRILSPVGIRLYRWAPA
                     GPVCPNCRREDDWVEQVGTHEIYCRCQVTG"
     gene            1785..>2416
                     /locus_tag="VF276_13070"
     CDS             1785..>2416
                     /locus_tag="VF276_13070"
                     /inference="COORDINATES: protein motif:HMM:NF024426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3887 domain-containing protein"
                     /protein_id="HEX6607835.1"
                     /translation="MASAANQSDNPADVSVPTTTEGAGPPAAPEVAAEPAAAPDGPRV
                     SGPILPMPAAAIGMANPLKSGREVAAQHFVNLLARGYYAEAAESFAPDVRAAVPPEAL
                     ESAWLRLTDQVGPLKRHLAATGGRLLQHDVVRDTCEFAATTLDLQVIFDDAGYVAGFQ
                     WLPLPGEHISDLLYDPPEYVRQAAFHERAVEVGAPPWTLPGTVSLPMGDGP"
BASE COUNT          363 a          795 c          866 g          392 t
ORIGIN      
        1 ctgtaggggc cggtttcaaa ccggcccgtg cgcccttcgg ggacgagact cttcgctgcg
       61 ttcagcgtga caagcctctg cctccggcgc caagactctt cgctgcactc cgagtgacaa
      121 gggcaggggt tacctcgcgc ccgctgtagg ggccggtttc aaaccggccc gtgcgccctt
      181 cggggacgag actcttcgct gcgctcagag tgacaagcct tctactgacc actgaccacc
      241 gaccagtcat cggccgtgcc cggtgtgacc aatggccgcg gatgccgtct tggaaggatg
      301 atgggcgcgt ggggcggcag gcggtgacaa atgccccgcc cccgccgtct ggaaggtatg
      361 gggcactggt tcggctgccc ggcaggccgg gttagaattc gacctgccgg gcggtgccgc
      421 ggagtgtcag ggcgtgatag ctctggcgca ccggggccac ggtctcggtt tgcgtggtgg
      481 tgagaatggc gacggtttgc agcgctgctt ccgtctcttc cccacgcagg ttaatcgccg
      541 ggatcagcag ggtcaggcgg cgattgtgga tcgccgtcac ctcgacgacg atggtgacct
      601 gggtgccgat ctcggtttcc tctgtgcgaa tatccggccc gccggcggtg acgttgtgtt
      661 gccgatcctg gtagtggagt tggggtttgc cggcgaagcc ggtgctcgaa taggtgatct
      721 gcgtatgctc gccgtgcagc acgtactggt taggctggga catggtcgta tcctccatga
      781 gtggccgcgg cggatggggc cggctgccga cccgcggcgc cggacgcgcc cggtcgctgc
      841 tgccccgctt cgtctgctta cctgaatagc gcggatgacg ggcaggttac gggacaaagg
      901 aaagcgcgcc gccggggatg gagtgtgcta tgatagcgcc aggaggtgcg ctatggcaaa
      961 agaagtgatc ctgaccctgg cggacgatac atacgcggca ttggcggcct gggcgggcgg
     1021 cgcggaccag gtcgccccgt atctacaacg gctggtggag gcgatactgg cccacccggc
     1081 gcaggaagag caacccgtac ggcgggggcg cgcgccgggc ctgctcgcca tgccggccga
     1141 cgccgacgac gatgacccgt tccatctgct ggccgaagat cagcgctggg gcgggccggg
     1201 cggctgaatc ccgtccggcg gatgtcttcg aataactaag ccaggccccg gcggccgccc
     1261 cggctgccgg actacatggt gccctggctg gaagcgcagc gtccgccggc gttgcgggaa
     1321 aggatcgctt catgaacctg cagcaagcca tgcttgcggc ccaattggcc catgccaggg
     1381 cgacggccgc ccaggacctg atcgacgata tggacagaat actgagccct gttggcatcc
     1441 ggctgtatcg ctgggcgccg gcggggccgg tctgtcccaa ctgccggcgg gaagacgatt
     1501 gggtcgagca ggtcggcacg catgagatct attgccgctg ccaggtaacc ggctagcccc
     1561 cctgcgcccg gactggtccc ggccccgccg tgataacctc tcgctgccgc cgttctaact
     1621 gcaaagtcct tatgagcagg gcggggccac cggcaaccgg ccctgtgtgc tagcagcatc
     1681 cattggccga tcgtgcggca ggcggacgtc cgctgccccc agcgccgccc ctcgcggatg
     1741 cgccaggcgg cccggccggt caaggggtgg aaaggagtcg gcgcatggcg agcgcggcga
     1801 atcagagcga taatccggcg gacgtgagcg tccccacgac cacggagggc gccggcccgc
     1861 ctgccgcccc cgaggttgcc gcggaacccg cggcggcgcc ggacggaccg cgcgtgagcg
     1921 gaccgatcct gccgatgccg gcggcggcca tcggtatggc gaacccgctc aagagcgggc
     1981 gcgaggtggc cgcgcagcac ttcgtgaacc tgctggcgcg cggctattat gccgaggccg
     2041 ccgagagttt cgcgcccgac gtgcgcgccg ccgtcccccc ggaggcgctg gaatccgcct
     2101 ggctgcggct gaccgaccag gtggggccgc tcaagcgcca tctggcggcg accggcgggc
     2161 ggttgctgca acacgacgtg gtgcgcgaca cctgcgagtt tgccgccaca acgctggacc
     2221 tccaggtcat cttcgacgat gccgggtatg tggccggctt ccagtggttg cccttgcccg
     2281 gcgaacacat ctcggacctg ctgtacgacc cgccggagta tgtgcgccag gcggccttcc
     2341 atgagcgcgc cgtcgaggtc ggcgcgccgc cctggacgct acccggcacc gtcagcctgc
     2401 cgatgggcga cgggcc
//
LOCUS       DASUYC010000798         2634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_2064226, whole genome shotgun sequence.
ACCESSION   DASUYC010000798 DASUYC010000000
VERSION     DASUYC010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2064226"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(273..1772)
                     /locus_tag="VF276_13075"
     CDS             complement(273..1772)
                     /locus_tag="VF276_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6607836.1"
                     /translation="MKIWRAFLALLTLAAMLTPTAGAAAQTAGPLDKINHLIVIYQEN
                     WSYDSLYGEFPGANGADNAGPAAQQVDKNGQPYTTLPQPINNNLKPAAADSRFPANLP
                     VGPFDIGKYVPADQKTGDPGARFYQEQSQIDGGKMDKFVALSTGAGLAMGYYDATNMP
                     EGKLAQQYTLADNFFHAAFGVSYLNHFWLISASTPVWPNAPANQVIQLDANGKLVKDG
                     VVTPDGYAVNTAFTVNTPHPASITDTAQLLPEQTMPTIGDRLSDKGISWAWYSGGWND
                     AIAGHPDPLFQANHQPFAYFANYADGTPGRAAHLKDENDFMRALTTNDLPAVSFIKPI
                     GADNEHPGYTNLLQGQQHVADLVSAVQNSPYWADTAIVITYDENGGFWDHVAPPKGDR
                     WGPGTRVPAIVVSPFAKKGFIDHTQYDTTSILRTIELRWNLAPLGTRDAAAAPLTNAF
                     DSGAPIPAGMPNTGNPADLSTALWLAGATGLLLALGGLALYRRAALHRQ"
     gene            2104..2631
                     /locus_tag="VF276_13080"
     CDS             2104..2631
                     /locus_tag="VF276_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6607837.1"
                     /translation="MQEQTKVARFEQAVLPHLNAAYNLARWLTRNDQDAEDVVQEAYL
                     RAFKFFDGYRGGDSGAWLLTIVRHTCYTWLQQRGAADLTTAFDEELHSDGCETDNPER
                     LAIQHADAQVLREALEALPVEFREVVVLRDLEGLSYKEIATVAAVPLGTVMSRLARAR
                     KRLQLWLAPRMSKER"
BASE COUNT          401 a          767 c          972 g          494 t
ORIGIN      
        1 gtcgcgggcg acgcgggccg gtttccaacc ggcccctaca gcgcgatagc gcgctgtcac
       61 tctgagcgga gcgaagagtc tcgtctcgtc gccacggaga cggagatgct tcgctgcgct
      121 cagcatgaca agggtagccc ggtgccggtt ccagcaagct atgggatgta gttgcgcgat
      181 ttatcgcgcc gtactcgttg gtcgtgagtg taggctgtcc ccaacaggtc gctgcaatcg
      241 gcaccaggca tctccgcgag ataccgggcg ggctactggc gatgcagcgc ggcgcgacgg
      301 tagagcgcca gcccgccgag cgccaggagc aggcccgtgg ccccggccag ccagagcgcg
      361 gtgctgagat ccgcggggtt gccggtgttc ggcatgcccg cggggatggg ggcgccggag
      421 tcgaaggcgt tggtcagcgg ggcagcggcg gcatcgcggg tgcccagcgg ggccaggttc
      481 cagcgcagtt cgatggtgcg caggatggag gtcgtgtcgt actgcgtgtg gtcgatgaag
      541 cctttcttgg cgaagggcga gacgacgatg gcggggacgc gggtgcccgg cccccagcgg
      601 tcgcccttgg gcggggcgac gtggtcccag aagccgccgt tttcatcgta ggtgatgacg
      661 atggcggtat cggcccagta ggggctgttt tgcacagcgc tgaccaggtc ggcgacgtgc
      721 tgctggccct gcagcaggtt cgtgtagccg gggtgctcgt tgtcggcgcc aatgggcttg
      781 atgaaggaga cggcgggcag gtcattggtc gttagggcgc gcatgaagtc gttctcatct
      841 ttgaggtggg cggcgcgacc gggcgtgccg tcagcgtagt tggcgaagta ggcgaacggc
      901 tggtggttgg cctggaagag cgggtcaggg tgcccggcga tagcgtcatt ccaaccgccg
      961 gagtaccacg cccacgagat acccttgtcg ctcagccggt cgccgatggt cggcatggtt
     1021 tgctcgggca gcaactgggc cgtgtcggtg atgctcgcgg ggtggggcgt gttgaccgtg
     1081 aaggcggtgt tgacggcata cccgtccggg gtcacgacgc cgtccttgac cagcttgcca
     1141 ttggcgtcaa gctggataac ctggttggcg ggggcgttgg gccagacggg cgtgctggcg
     1201 ctgatcagcc agaagtggtt gaggtacgag acgccgaagg ccgcgtggaa gaagttgtcg
     1261 gccagcgtgt attgctgggc cagtttgccc tcaggcatat tggtggcgtc gtagtagccc
     1321 atcgccagcc cggcgccggt gctcaaggcc acgaacttat ccatcttgcc gccgtcgatc
     1381 tgcgactgct cctggtagaa gcgggcgccg gggtcgccgg tcttctggtc ggcgggcaca
     1441 tacttgccga tgtcgaaagg tcccaccggc aggttggccg ggaagcgcga gtcggcggcg
     1501 gcgggcttga ggttgttgtt gatcggttgg ggcagcgtcg tgtagggctg cccgttcttg
     1561 tccacctgtt gcgcggcggg accggcgtta tccgcgccgt tcgcgccggg gaactcgccg
     1621 tagaggctgt cgtagctcca gttctcctga taaatcacga tcaggtggtt gatcttgtcc
     1681 agcggtcccg ctgtttgcgc cgcggcgccc gcggtcgggg tcagcatggc cgccagggtg
     1741 agtagcgcca ggaatgcgcg ccatattttc atcgtcttat cctccatttt cgctatttct
     1801 gttgggccgc gttgcgcggg ccgggcgatc tcgtcctgcc cacacccccc tataccggcg
     1861 gagtggggaa attattcccg ccgggtgtga tacaatggaa gatagattgt ctttgcctcg
     1921 cgcggccaat gtggggaata aaaccagcgg gtcctcagtc tggatgggta gaccggccta
     1981 ttgccgggcc ggagccggcg accgtttatg cgctctttgc tgcctgccgc cagcgtccac
     2041 gatgcaatag cacgcggcgc gcgatcgcct gacgcgaccc gcgcacgctg gagaggcaca
     2101 cggttgcaag agcagaccaa agtcgcccgg tttgaacagg cggtcttgcc gcacctgaac
     2161 gcggcgtata acctggcccg ctggctgacg cgcaacgacc aggacgccga ggacgtggtg
     2221 caggaggcgt atctgcgcgc gttcaagttc ttcgacggct accgcggcgg ggacagcggc
     2281 gcctggctgc tgaccatcgt gcgccacacc tgctacacct ggttgcagca gcggggcgcc
     2341 gccgatctga ccacggcgtt tgacgaggaa ttgcatagcg acggttgcga gaccgataac
     2401 ccggaacggc tagcgatcca gcacgccgac gcccaggtat tgcgcgaggc gctggaggcg
     2461 ctgccggtgg agtttcgcga ggtggtggtg ctgcgcgacc tggaagggct gtcctataag
     2521 gagatcgcta ccgtcgccgc ggtgccgctg ggcaccgtca tgtcccgcct ggcgcgggcg
     2581 cgcaagcggt tgcagctttg gctggccccg cgcatgagca aggagcggta acgt
//
LOCUS       DASUYC010000799         3751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_819504,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000799 DASUYC010000000
VERSION     DASUYC010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819504"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1215)
                     /locus_tag="VF276_13085"
     CDS             complement(<1..1215)
                     /locus_tag="VF276_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEX6607838.1"
                     /translation="MANPDVKEDSAPSVTAQPDLLDRVRSARLVQWLRRDVLAGSGFE
                     SQRYLAKWLFLGTLIGVVAGLGAVAFYLGIAWATDFFLGTLAGYLPPAPAGEGVTRVV
                     APDRPWLLPAVTALGALISGLIVFTLAPEAEGHGTDAAIDAIHHKWGRIRARIPPIKL
                     VASAITIGSGGSGGREGPAAQISAGFGSLLSDWLGLDTQDRRIAVAAGMGAGIGAIFR
                     APLGGALMAAEILYLHDLEVEAIIPGLIASIVGYSVYGAFFGYMPIFGAQTQLAFDQP
                     IQLVYYGLLGVLSGLGGLLYARSFYGISGMFHRLALPRWLKPALGGGLVGLLGIWVPG
                     ALHMGYGWVQIGMSRDLLGLPLWMVLVLPLAKILATSLSIGSGGSGGIFGPGMVIGGM
                     LGAAFWRLGQGVL"
     gene            complement(1337..2344)
                     /locus_tag="VF276_13090"
     CDS             complement(1337..2344)
                     /locus_tag="VF276_13090"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX6607839.1"
                     /translation="MTTIRDVARVAGVGIGTVSRVLSGHPNVAEATRQRVQAAIEQLD
                     YRSSPVARALRRGRTGVIEVLVPLFTRHFYVEVLRGIAEAATTGGYTLLIRTIERPAD
                     RDRAFAACGAAGTADGVLLVSLAPPAALLHRLAAAGVPLVLVDRTDGGPASIGVDHAA
                     AMAAAVGHLVGLGHRRIALIDRREDPFALEEPGGRQAGYRQALAEAGLPLNPAYDMVT
                     EYTPEAGQAALAALLTLPVPPTAVLTGSDGQALGVLEAARQAGRAVPGDLSVAGYNDI
                     DVAPYLGLTTVHVPMYDMGRAGSEMLLDLLDRPGVAPAPRRFPADLVVRATTGPAPPG
                     G"
     gene            complement(2517..3443)
                     /locus_tag="VF276_13095"
     CDS             complement(2517..3443)
                     /locus_tag="VF276_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607840.1"
                     /translation="MKRLDPAHYLEAPALELDERKRAAFDAMYQAALDQGPNSVIDYR
                     ADYPKHEFLTYLVEHKGVLLHGSNNGGITRLKPKWQTDYLGKPVKAIFATPDGIWPMF
                     FAILDREVYQGSLQNGCFWVHDPEGAAHKCYHFSINAAALPHEPWTDGTIYVVPRDSF
                     RRGHDLADQPLEEWTSREPVAPLARLPVSPEDFPFLDQIEGHDEATLERLEREPDELF
                     KGFHDLAELDDGYELRYDGTAQWDGQLMEFIRRQRKQSPALTYELVFEPDEGPIRLRL
                     RGTHAAKPEIQDKINSFVDARNTPDPAAPPGG"
BASE COUNT          605 a         1342 c         1264 g          540 t
ORIGIN      
        1 cagcacgccc tggcccagcc gccagaaggc cgcgcccagc atcccgccga tgaccatgcc
       61 gggaccgaaa atgccgcccg accctcccga gccgatggac agcgaggtcg ccaggatctt
      121 ggcgagcggc agcaccagca ccatccagag cggcaggccc agcaggtcgc ggctcatgcc
      181 gatctgcacc cagccatagc ccatgtgcag cgcgcccggc acccagatgc ccagcaggcc
      241 gacgaggccc ccgccgagcg ccggcttgag ccagcgcggc agggccagac ggtggaacat
      301 cccgctgatc ccgtagaacg accgcgcata gagcagcccg cccagcccgc tcagcacgcc
      361 gagcaggccg taatagacca attggatcgg ctggtcgaac gccaactgcg tctgcgcgcc
      421 gaagatcggc atatagccga agaaggcacc gtagacgctg tagccgacga tggacgcgat
      481 caggccgggg atgatcgcct cgacttcgag gtcgtgcagg tagaggatct ccgccgccat
      541 tagcgcccca cccagcggcg cgcggaagat ggcgccgatg cccgcgccca tgcccgccgc
      601 cacggcgatg cgccggtcct gcgtgtcgag gccgagccaa tcgctcaaca gcgacccgaa
      661 gcccgccgaa atctgcgccg ccggcccctc gcgccccccc gatccgcccg acccgatggt
      721 gatggccgag gcgaccagct tgatcggcgg gatccgggcg cggatgcggc cccacttgtg
      781 atggatggcg tcgatggccg cgtcggtgcc gtggccctcg gcttccggcg ccagcgtgaa
      841 gacgatcagg cccgagatga gcgcgcccag tgccgtgacg gcgggcagca gccagggccg
      901 gtccggcgcc accacccgcg tcaccccttc cccggcgggc gccggcggca gatagccggc
      961 cagcgtgccg agaaagaaat cggtggccca ggcgatgccg agataaaagg ccaccgcgcc
     1021 cagcccggcc acgacgccga tcagcgtgcc caaaaacagc cacttggcga ggtagcgctg
     1081 gctttcgaag ccgctaccgg ccagcacatc gcgccgcagc cactggacca ggcgcgcgct
     1141 gcgcacgcgg tccagcagat cgggctgggc cgtcaccgaa ggcgcggaat cctccttgac
     1201 atcggggttc gccatttacc gttcctccgg aaagtcgtcg cgcagacacg catatccgaa
     1261 tatccgcccg aacacaaaaa cataaacgcg gcgccgcgcg ccgccttatc cgagctacca
     1321 atgcggaatc cgcccatcac ccgccgggcg gcgcggggcc agtggtggcg cggaccacga
     1381 gatccgccgg aaagcggcgc ggcgccggcg caacgcccgg ccgatccagc agatcgagca
     1441 gcatctcgct gcccgcgcgg cccatgtcgt acatcggcac atgcaccgtc gtcaggccga
     1501 gatagggcgc tacgtcgata tcgttgtagc cggcgaccga gagatcgccg ggcaccgcgc
     1561 ggcccgcctg ccgcgccgcc tccagcaccc ccagggcctg gccgtcgctg ccggtcagca
     1621 cggcggtggg cgggaccggc agcgtcagga gcgccgcgag cgcggcctgc ccggcttcgg
     1681 gcgtgtattc agtcaccata tcgtacgcgg gattcagcgg cagcccggcc tcggccagcg
     1741 cctgccggta gcccgcctgc cgtccgcccg gttcctccag cgcgaagggg tcctcccgcc
     1801 ggtcgatcag cgcgatgcgc cggtggccca ggcccaccag gtgccccact gccgccgcca
     1861 tggccgcggc atggtccacc ccgatgctgg ccgggccgcc atcggtgcgg tcgaccagca
     1921 ccagcggcac cccggcggcg gcaagccggt gcagcagcgc ggcgggcggc gccagcgaca
     1981 ccagcaacac gccatccgcc gtgcccgccg ccccgcaggc ggcaaacgcc cggtcacggt
     2041 cggcggggcg ctcgatggtg cggatcagca gcgtgtagcc ccccgtcgtc gccgcctcgg
     2101 cgatgccgcg cagcacctcg acgtagaagt ggcgggtaaa cagcggcacc agcacctcga
     2161 tcacgcccgt gcgcccgcgg cgcaaggccc gcgccaccgg gctggaccgg tagtcgagtt
     2221 gctcgatagc cgcctgcacg cgctgtcgtg tcgcttccgc cacgttgggg tgcccgctca
     2281 gcacccgcga caccgtgccg atgcccaccc cggccacccg cgccacatcg cgaattgtcg
     2341 tcatgccgcc gccctccctt gctggaaacg attccgcacg ccattatacc gatcccgctg
     2401 ccccgcggca agggacgccc cggcaatcaa gggtcgggct acgtcaccga gcccgcttgt
     2461 acgggctcgg tgaattgagg ccgcgcaggc ggccttcgtg gagctagcgg gcggctttag
     2521 ccgcccggcg gtgccgccgg gtcgggggtg ttgcgggcgt cgacgaagct gttgatcttg
     2581 tcctggatct cgggcttggc ggcgtgcgtc ccgcgcagac gcagccggat cggcccctcg
     2641 tccggctcaa acaccagctc gtaggtcagg gcggggctct gcttgcgctg gcggcggatg
     2701 aactccatca gttgcccgtc ccattgcgcg gtgccgtcgt agcgcagttc atagccgtca
     2761 tccagttcgg ccaggtcgtg gaagcccttg aacagttcgt cgggctcacg ctccaggcgc
     2821 tccagcgttg cctcgtcgtg gccctcgatc tgatccagga acgggaagtc ctccgggctg
     2881 accggcagcc gggccagcgg cgccaccggc tcgcggctgg tccactcctc cagcggctgg
     2941 tcggccaggt cgtgcccgcg ccggaacgag tcgcgcggca ccacgtagat ggtgccgtcg
     3001 gtccacggct cgtggggcag cgcggcggcg ttgatcgaga agtggtagca cttatgggcg
     3061 gcgccctccg ggtcgtgcac ccagaagcac ccgttctgca acgacccctg gtagacctcg
     3121 cggtcgagga tggcgaagaa catcggccag atgccgtcgg gcgtggcgaa aatcgccttg
     3181 accggcttgc ccaggtagtc ggtctgccac ttgggcttga gccgcgtgat gccgccgttg
     3241 ttcgagccgt gcagcaacac gcctttgtgc tcgaccaggt aggtcaggaa ctcgtgcttg
     3301 gggtagtccg cccggtagtc gatgacgctg ttcggtccct ggtccagggc cgcctgatac
     3361 atggcgtcga aggcggcgcg tttgcgctcg tccaactcca gggcaggcgc ttccaggtag
     3421 tgcgctgggt ccaggcgttt catcccggtt tcctcattga gcggcggcat gagtggacag
     3481 attggtatgt agtggcgcga tttatcgcgc cgtactcgtt gatcttcagc gtgttgcgtc
     3541 tcccatacgt tttcggaagc tgcattggtc acaggagcta tagcaaatgt cgcgccgcgt
     3601 gacatttgac gactggcggc gcagatgggt taagataggc gcttatacaa cgtgggcgcc
     3661 ggggtcgggg tggcgggcac ggctggcaca aaggttgctc ccccgcccgg caagacccca
     3721 aggaggtatg tatggaccaa gccgaagagc a
//
LOCUS       DASUYC010000800         3613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1724088, whole genome shotgun sequence.
ACCESSION   DASUYC010000800 DASUYC010000000
VERSION     DASUYC010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1724088"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VF276_13100"
     CDS             complement(<1..275)
                     /locus_tag="VF276_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6607841.1"
                     /translation="MNLLLVEDNERLRQALKLGLQGTGEVVVTQDCASGEEALAACLA
                     ARPDVVLMDVQLAGAMNGITAAVALRREFPRLPVVFYSIQDDENYYR"
     gene            418..1971
                     /locus_tag="VF276_13105"
     CDS             418..1971
                     /locus_tag="VF276_13105"
                     /inference="COORDINATES: protein motif:HMM:NF025067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HEX6607842.1"
                     /translation="MRTTTVLPVLPAPVVRTPVRASPQARLLLAGVGLGGAVDLLFYR
                     QALGVSLLVFVGLVLGTLFGLSRVEDVRPAWRNLWLLGPLVFFAGMVAVRANAFLTAL
                     NVLATLALLGLLAYFYAASRVTRLDLIGYPLALLAVLGNAFLQPAPLLPATVTVAREH
                     RRGVGPLLPVLRGLALALPVLLLFSCLLASADVVFARYLGAVLQFDFLPDFGDLFAQG
                     LIVTGAGWFLAGALVYALRRRPGAGEGFPWEPETAALRGMASLGFVEVVTILSAVNLL
                     FLAFGAIQFAYLFGGRANITLAGFSYADYARRGFFELVAVSVLTLGLILGLQWLVRPR
                     TPAQTWLFNGLSTLMIGLVLVLLASALQRMVLYEEAYGYTHLRLYVHVFMLWLAATFL
                     WLLGTLWVWPRRFAIGAFVCALGFLATLNVVNPDVTIARENLGRYQAAGRLDALYLTD
                     LSDDAVPLLMASLDTLQGEDRALLRARLLGLARREPTPGTQTWPAFHLARQTAATVLA
                     QNRARLEQP"
     gene            2226..3110
                     /locus_tag="VF276_13110"
     CDS             2226..3110
                     /locus_tag="VF276_13110"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6607843.1"
                     /translation="MNETRTLIRNGHLALDRLNEWGARPTLFAPGEAQFWDDPHISAQ
                     MLQAHLDPNVDAASRKPETIDRTVDWLVARLRLQPSDSVLDLGCGPGLYCTRFAARGL
                     RVTGIDYSRRSIDYAIEHAPVGEPVIEYIYGNYLETDYGAPGPCFNAAVLIYGDFCTL
                     DDAARDLLLTRIHAALVPGGYFAFDVMTPVHHERSGPATAWSAEKSGFWRAAPHLVLE
                     QRFDYPDADVFLHRYLVIESSGALTDYRLWYRLYTSETIGLVLDRAGFTLCEAQVDLA
                     GTVYDPASEWLGLVARKR"
     gene            complement(3165..3238)
                     /locus_tag="VF276_13115"
     tRNA            complement(3165..3238)
                     /locus_tag="VF276_13115"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3202..3204),aa:Asp,seq:gtc)
     gene            complement(3248..3364)
                     /gene="rrf"
                     /locus_tag="VF276_13120"
     rRNA            complement(3248..3364)
                     /gene="rrf"
                     /locus_tag="VF276_13120"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(3501..>3613)
                     /locus_tag="VF276_13125"
     rRNA            complement(3501..>3613)
                     /locus_tag="VF276_13125"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          503 a         1164 c         1224 g          722 t
ORIGIN      
        1 tcgcgatagt aattctcgtc gtcctggatg gagtagaaga cgaccggcag gcgcgggaac
       61 tcgcggcgca gggccacggc ggcggtgatg ccgttcatgg cgcccgccag ttgcacgtcc
      121 atcagcacga catcggggcg cgcggccagg caggcggcca gcgcttcctc gccgctggcg
      181 cagtcctggg tcacgacgac ttcgccggtg ccctgtagcc ccagtttgag cgcctggcgc
      241 aggcgctcgt tgtcttcgac cagtaagagg ttcatcatgg cttcattgtg ggtgtttagc
      301 ggtacgtgtc aagcactcag gtgcgccgtt gcgccgcctt agagcatggc ggcacgcggc
      361 cagagtgggt aagatgggca acgggtccat acattattat acctggagag cctgatgatg
      421 agaactacta ctgtcttgcc cgtcttgccc gcgccggtgg tgcgcactcc ggtgcgggcc
      481 agcccgcagg cgcggctgct gctggccggg gtgggcctgg gcggcgcggt cgatctgctg
      541 ttctaccggc aggcgctggg cgtgtcgctg ctggtgttcg tggggctggt gctgggcacg
      601 ctgttcgggc tgagccgggt ggaggatgtg cgcccggcgt ggcgcaacct gtggttgctg
      661 gggccgctgg tcttcttcgc cggcatggtg gcggtgcggg ccaacgcctt cctcacggcg
      721 ctcaatgtgc tggccacgct ggcgctgctg gggctgctgg cctacttcta tgccgcgagc
      781 cgggtgaccc ggctggacct gatcggctat ccgctggcgc tgctggcggt gctgggcaac
      841 gccttcctgc aaccggctcc gctgctgccc gcgaccgtga cggtggcgcg agaacaccgg
      901 cggggggtcg ggccgctgct gccggtgcta cgcgggctgg cgctggcgct gccggtgctg
      961 ctgctgttta gctgcctgct ggcctcggcc gatgtcgtgt tcgcccgcta cctgggcgcc
     1021 gtgctgcaat tcgacttcct gcccgacttc ggcgacctgt tcgcgcaggg gctgatcgtg
     1081 accggggccg ggtggttcct ggcgggcgcg ctggtctacg cgctgcgccg ccgccccggc
     1141 gcgggcgaag gcttcccctg ggaaccggag acggcggcct tgcggggcat ggcgtcgctg
     1201 ggcttcgtcg aggtggtgac gattttgagc gccgtcaacc tgctgttcct ggcctttggc
     1261 gccatccagt tcgcctacct gttcggcggg cgggccaaca ttaccctggc cgggttcagc
     1321 tacgccgatt acgcccggcg cggcttcttc gagctggtcg ccgtgtcggt gctgacgctg
     1381 gggctgatcc tggggctgca atggctggtg cgcccgcgca cgcccgcgca aacgtggctg
     1441 tttaacggct tgagcacgct gatgatcggg ctggtgctgg tgttgctagc ctcggcgctg
     1501 caacgcatgg tgctgtacga agaagcctac ggctacacgc acctgcggct ctacgtccac
     1561 gtcttcatgc tctggctggc ggcgaccttc ctctggctac tgggcacgct gtgggtgtgg
     1621 ccgcggcgct tcgctatcgg cgccttcgtc tgcgcgctcg gcttcctggc gacgctcaac
     1681 gtggtgaacc ccgatgtcac catcgcgcgg gagaacctgg ggcgctatca ggccgcaggc
     1741 cggctcgacg ccctctacct gaccgacctc tcggacgatg ccgtccccct gctcatggcg
     1801 agcctcgata ccctgcaggg ggaggaccgg gcgctgttgc gggcgcggct gctgggcttg
     1861 gcacggcgtg agccgacccc cggcacgcag acctggccgg ctttccactt ggcccgccag
     1921 accgcagcca ccgtgctggc acagaaccgc gcccggctgg aacagccgta gcgcgcagat
     1981 cgcgcacggg cagagctatg cgcgtcgctc tgcccgtgct tgcgattcta ggcggggtta
     2041 cgctctcttc accccccagc acgtcctctt tacgctctgc ttgcttgcct tctgctaaac
     2101 tctacttcgt aggctattgc cctctaccac ggcaaagggt gggcgggttg ggccgatcgc
     2161 aaactggccc ccacacagcc ccagcaccgg ggtcacactg cgggtagtca cccgataggt
     2221 gagggatgaa cgagacgagg accctgatcc ggaacggcca cctggcgctt gatcggttga
     2281 acgagtgggg ggcgcggcct accctgttcg ctcctgggga ggcgcaattc tgggatgacc
     2341 cgcatatctc cgcgcagatg ttgcaggcgc atctggatcc gaatgtggac gccgccagcc
     2401 gtaagccgga gacaatcgac cgcactgtgg actggttggt ggcgcgcttg cgcctccagc
     2461 cgagcgacag tgtgctcgac ctggggtgcg ggccggggct atattgcacg cgcttcgccg
     2521 cgcgcggctt gcgggtgaca ggcatcgact actcgcggcg gtccatcgac tacgcaattg
     2581 aacacgctcc tgtgggcgaa ccggttatcg aatatatcta cggcaactat ctggagacgg
     2641 actacggggc gcccggccca tgcttcaacg cggctgtcct gatctatggc gatttctgca
     2701 cgctggacga cgcggcgcgg gatctcctgc tcacccggat acacgcggct ctcgttccgg
     2761 gcggttattt cgcttttgac gtgatgacgc cggtgcatca cgagcggtcc ggtcccgcca
     2821 cggcctggtc ggcggaaaaa agcggcttct ggcgggcggc accccacctg gtgctcgaac
     2881 agcggtttga ctacccggac gcggacgtat tcctgcaccg gtatctggtg atcgaatcgt
     2941 ccggtgccct taccgactac cgcctctggt accgcctgta tacttccgaa accatcggcc
     3001 tcgtgctgga tcgcgcgggc tttaccctgt gcgaggcgca ggtggatctg gccgggacgg
     3061 tttacgatcc ggcgtcggaa tggcttggcc tggtggcacg gaagcggtag agaatatcgg
     3121 acaaaaaaag accgcaccct gatatcagga tgcggtcatt agggcggaag cgacgggatt
     3181 cgaacccgcg gtctcagcct tgacagggct gcatgttagg ccgctacacc acgcctccaa
     3241 aaagagagac ccgcgacggc ctagtctccc acccccttga ggaggcagta ccctcggcgc
     3301 tgcaacgttt cacggccagg ttcgggatgg aactgggtgg gtccgctgcg ctcttgtcac
     3361 ggatctctac ttttacactg ttagcccgca taggttcttg tcacgctcat cattgttcgc
     3421 tgcgtcttgg ccgccttgcg tgtgcgccgg cttccggccc ctgctccttg ccgagcagca
     3481 gaccgaagcc cttggcgatg ttgccacaaa ccctcgacca tgagcacgcc ttggctggcc
     3541 ggttacccgg tttccacctg acgcctcttt gccgggtcgt ctgcccgggg tcttactggc
     3601 ttgcgccatg aga
//
LOCUS       DASUYC010000801         5119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1512164, whole genome shotgun sequence.
ACCESSION   DASUYC010000801 DASUYC010000000
VERSION     DASUYC010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5119
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512164"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            331..774
                     /locus_tag="VF276_13130"
     CDS             331..774
                     /locus_tag="VF276_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607844.1"
                     /translation="MSASISVAGSLARLSQEDGPASGMSKSPAYTTARPRCLVLAENT
                     SLRLLVRMILSLEGWEAVEQEGSAYQAVVADLDCFVWPGERVGLVVRQAMLAGLPVLA
                     LTGQDLNPQECAAIGNPPLLMKPFELASFIQVLHTWRLGLESRVA"
     gene            1088..1621
                     /locus_tag="VF276_13135"
     CDS             1088..1621
                     /locus_tag="VF276_13135"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX6607845.1"
                     /translation="MIETAPTSEAPTAAPARPAVDQAAGRVLRVIPVMFKQMIVKARE
                     EMPDALCDLGETQFRIIHALNYGSFTMGELAERMKVRTPTVSRMIDAMVARGLVDREP
                     DSTDRRKVWLHLTAEGRKVSTAMEQHFQTAVARFLQPLDDEKLAVIVAACDALEGLLS
                     SPQDNEPKHSTVREDSR"
     gene            1621..3360
                     /locus_tag="VF276_13140"
     CDS             1621..3360
                     /locus_tag="VF276_13140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEX6607846.1"
                     /translation="MAAQVQDRRPAAAASANEEPPVKNLLIIMIGIMLGVLLGALDQT
                     VVGPAMPKIIGDLNGFDHYAWVFTAYMLTSTISVPIFGKLGDMYGRKWFYVAGILIFL
                     AGSMLCGLSQDIWQLIAFRAFQGLGGGIMFSNAFTIIGDLVPPADRGRWQGLFGGVWG
                     LASVFGPTAGGYITDNLSWHWVFYVNVPVGIVALAVLLTTFPNMHHDTGAKKVIDWFG
                     ALTLIGAMTPLLVGLSLAGTADDWTWTSFNTLATFAVAAIFLVAFLFVETRVSQPIIP
                     LDLFKNRTFTLSVITVFLTGIGMFGAVSYIPLFIQAVQGGSATSSGNAITPMMLTVVF
                     SSVVTGQLISRTGKYRVLGVVGMVLLTGGMFLLAGMDTSTPTWQTVIYMMVMGLGLGV
                     AMPLYNLVVQNAFPITRLGVVTSAVTFFRSIGGTVGIAVLGSIVNNRFRDESVNQIQQ
                     LNPQLASQLPAEFVSKLNPQVLVNPQAIEGMRQAMQAQHVPAALINGIIDTILKAMKP
                     ALAIATTEAFLLGAVTLIIAIAATAFIQEIPLRKTNARPAMAMAEGDRPDVVQESEEA
                     LEAKEPVAVGGRR"
     gene            3776..>5119
                     /locus_tag="VF276_13145"
     CDS             3776..>5119
                     /locus_tag="VF276_13145"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX6607847.1"
                     /translation="MHLLTKGALSRIAIVLLVGVFVIAGGIYSATQLKSELLPSIDIP
                     SVTVITIYPGAAPDDVLRDVTEPLERALAGTENLKTTNSTSNDNLSIVTLQYEYGTDM
                     EKAQQRVQDLVNRVTLPAQVQRPTVSRFNFSDIPLAAYTLNTSDIGPDALSNLRRKAN
                     EEIVPELQGIPGVNSVTVSGGSNKQVIITLDAKKLAEKGLTASQVTGVLQANNVSFPA
                     GTIVTGGQSVPVIVGHEFQSVDDLRKVVVGMQMPAGMGAPGAAGGAGAAGSPAGGTMP
                     GAGGAPGGAGAPNSAAPGGAATGAAGTTAPGASGVVTGTTAPAGNAGGAKAGSSTGKT
                     GSGTATGGSTGKAGSPAGNATPGPATGGAAAGNAGAGAATGGAATGAQGTAAQGSAGG
                     APAAAATPVAPTPVELQDVATVELTNSAASTVSRSNGKPSIALIVYKTQNANTVQA"
BASE COUNT          946 a         1665 c         1628 g          880 t
ORIGIN      
        1 cgaacccgac cccaccgcgc cgcgctatct gctgacccgg cccggcttcg gttacacgat
       61 gcccgtctag cgaattcgga ttcgccgccc gatgagtcac gcgttcgcca tttggtgatt
      121 tgtttgtgac tttttcaggc cgtccgcaca cggcaaaaag acccttatcg tatatcatag
      181 agtaacaccg cctggcccga tggggccggg cgggcggggc ggctctccac gcgggcagcc
      241 ctgcgcgtgt tttgcgccac ggacgggcgg cgcagcgaag taccccccat ttactacggc
      301 atccccagat gccaagcaga ggcaagcgga ttgagcgcat cgattagtgt ggccgggtcg
      361 ctggcgcggc tgtcccagga ggacggcccc gcatccggta tgagcaagtc accagcctat
      421 accaccgcgc ggccccgctg cctggtactg gccgaaaaca ccagcctgcg cctgctcgtg
      481 cgcatgatcc tctcgctcga aggctgggaa gccgtcgaac aagaaggctc cgcctatcaa
      541 gccgtcgtcg ccgacctgga ctgcttcgtc tggccgggcg aacgggtcgg gctggtcgtc
      601 cgtcaggcca tgctagccgg gctgcccgtc ctggcgctca ccggccagga tctgaacccg
      661 caggagtgcg cggctatcgg caacccgccg ttgctgatga agccgtttga actggctagc
      721 tttatccagg tactccatac ctggcggcta ggcctggagt ctcgcgtcgc ctgattcgcc
      781 aactccatta aggcatcgtg ggccattcgc tgagaggcga gtggcccgtt ttttgcgtac
      841 cccactaccc tggaagtcct atacagtgta tacgtccagt ctttcgccaa ggtttgttgg
      901 ctcttgcgac gaactagcag atgtagtagt gtcggtcttt cggcggatct taggcagccg
      961 acgaggcaag aaggattgac agcatttgtt agatcgagta ttatttagca gtgctattaa
     1021 ttagccaggc taacattagc gatgaggctg atgtctgaat tttatgaagg acgcagagaa
     1081 accaaacatg atagaaaccg cacctaccag cgaggccccc accgccgccc ctgcccgtcc
     1141 ggctgtggat caggccgccg gccgcgtgct gcgcgtcatc ccggtcatgt tcaagcagat
     1201 gatcgtcaag gctcgtgagg aaatgcccga cgccctctgc gacctgggtg agacccagtt
     1261 ccgcatcatc catgccttga actacggaag cttcacgatg ggagaacttg ccgaacgcat
     1321 gaaggtccgc acgcccacgg tgtcgcgcat gatcgacgcg atggtggcgc gcggcctggt
     1381 ggaccgcgaa ccggacagca cagaccggcg caaagtctgg ttgcacctca ctgccgaggg
     1441 ccggaaggta tccaccgcga tggagcagca cttccagacc gccgttgcac gcttcttgca
     1501 accgttggat gatgaaaaac tggctgtcat tgtcgcagcc tgcgacgcgc tcgaagggct
     1561 actgtccagc ccacaggaca acgaaccaaa gcactcaact gttcgggagg actcacgtta
     1621 atggcagcac aagtgcaaga ccggcggccc gccgcggcgg catccgcgaa cgaggaaccg
     1681 ccggttaaaa acttactaat catcatgatc gggatcatgc ttggcgtgtt gctgggcgcg
     1741 ctcgatcaga cggtcgttgg accggccatg ccgaagatta tcggcgacct caacggcttc
     1801 gatcactacg cctgggtgtt caccgcctac atgctcacct cgacgatctc ggtgccgatc
     1861 ttcggcaagc tgggcgacat gtacggccgc aagtggttct acgtcgccgg tatccttatc
     1921 ttcctggccg gctcgatgct ctgcgggttg tcgcaggaca tctggcagtt gatcgccttc
     1981 cgcgccttcc agggcctggg cggcgggatc atgttctcca acgcctttac catcatcggc
     2041 gacctggtgc cgccggcgga ccgcgggcgc tggcagggtc tgttcggcgg cgtctggggc
     2101 ctggcgagcg tgttcggccc caccgcgggc ggctacatca ccgataatct gagctggcac
     2161 tgggtgttct atgtgaacgt gccggtgggc atcgtggcgc tggccgtctt gctgaccacc
     2221 ttccccaaca tgcaccacga caccggcgcc aagaaagtga tcgactggtt cggggcgttg
     2281 accctgatcg gggcgatgac tccgctgctg gtcggccttt cgctggccgg caccgccgac
     2341 gactggacct ggacctcgtt caacacgctg gcgacgttcg ccgtcgcggc gatcttcctg
     2401 gtggccttcc tgttcgtgga aacgcgggtg tcgcagccga tcattccgct cgacctgttc
     2461 aagaaccgca ccttcaccct gtcggtgatc acggtgttcc tgaccggtat cggcatgttc
     2521 ggcgcggtca gctacatccc gctctttatc caggcggtgc agggcggcag cgcgaccagc
     2581 agcggcaatg cgatcacgcc gatgatgctc accgtcgtct tctccagcgt ggtcacaggg
     2641 caactcatta gccgcaccgg taagtaccgt gtgctgggcg tcgtgggcat ggtcctgctg
     2701 accggcggca tgttcttgct cgcgggcatg gatacgagca cgccgacctg gcaaaccgtg
     2761 atctacatga tggtcatggg tctggggctg ggcgtggcga tgccgctcta caacctggtt
     2821 gtgcagaacg ccttcccgat cacccggctc ggcgtcgtga cctcggcggt aacattcttc
     2881 cgctcgattg gtggtacggt cgggattgcc gtcctcggct cgattgtgaa caaccggttc
     2941 cgcgacgagt cggtgaacca gatccagcaa ctcaacccgc agttggcgag ccagttgccg
     3001 gcggagttcg tcagcaagct taacccgcag gtgctggtca acccgcaggc tatcgagggg
     3061 atgcgccagg ccatgcaggc ccagcacgtc ccggcggcgc tgatcaacgg catcatcgac
     3121 acgattctca aggcgatgaa gcccgcgctg gcgattgcca ccaccgaggc gttcctgctc
     3181 ggggcggtga cgctgattat cgctatcgcc gccacggcct tcattcagga gatcccactg
     3241 cgcaagacca atgcgcgccc agccatggcc atggccgagg gggaccgccc cgacgtggtc
     3301 caggagagcg aagaagctct ggaggcgaaa gagccggtgg ccgtcggcgg tcgccgctaa
     3361 tcgttcgata ggggcgctgc gccgtgacct gtgcagcggc gcagcgcccc ctaatccccc
     3421 gcttccgttc caacaggccg ccgatatcgt tctttagaat atacttagcg gcctttttgt
     3481 gcgcgcaaga tttgtgaaaa tatacacaat atggtaaaga aggtgatgag ggtcgcgcgc
     3541 atatgatagc gtgcaacctc agggccttgc caacgtagga tttggcagat acaaagctga
     3601 aagccgccgg ccccatcccg gctatccatc atcggggatg ggcaccggac cgagcaacaa
     3661 atatagccaa cacgcgcctg gccgcaggcg cgtcccgccg gcacgcatcg ggtaggaaac
     3721 gcggtagccg gcacagcagc accgggctga caatacacac ggaaggaagt agtacatgca
     3781 cctgctaacc aaaggcgccc tgagccgaat cgcgattgtg cttctggtcg gggtctttgt
     3841 tatcgccggg ggtatctact cggcgacgca actcaagagc gagttgctgc cgagcatcga
     3901 tattcccagc gtcaccgtca tcaccatcta tcccggcgcg gcgcccgacg acgtgttgcg
     3961 cgatgtcacc gagccgctgg agcgggcgct cgcgggcacc gagaacctca agacgaccaa
     4021 ctccacgtcg aacgacaacc tctcgatcgt gacgttgcag tacgagtacg gcaccgacat
     4081 ggagaaggcg caacagcgcg tgcaagacct ggtcaaccgg gtgacgctgc ccgcgcaggt
     4141 gcagcggccc acggtgagcc ggttcaactt cagcgatatt ccgctggccg cctacaccct
     4201 gaacaccagc gacatcggcc ccgatgcctt gagcaacctg cggcgcaagg cgaacgagga
     4261 gattgtccct gagttgcagg gtattcccgg cgtgaactcg gtcacggtca gcggcggctc
     4321 gaacaagcag gtgatcatca cgctcgatgc caagaaactg gccgagaagg gcctgaccgc
     4381 gtcgcaggtg acgggcgtat tgcaggccaa taacgtcagc ttcccggcgg gcaccatcgt
     4441 gaccggcgga cagagcgtgc cggtgatcgt gggccacgag ttccagtcgg tggacgacct
     4501 gcgcaaggtc gtcgtcggca tgcagatgcc ggcgggcatg ggcgcccccg gcgcggcggg
     4561 cggcgccggc gcggcgggta gccccgcggg cggcacgatg cccggtgcgg gcggcgcacc
     4621 aggcggggca ggcgccccga acagcgcggc ccccggcggc gcggcgaccg gcgcggcggg
     4681 gaccacggcg cccggcgcca gcggcgtcgt caccggcacc acggcgcccg ccggtaacgc
     4741 gggcggcgcc aaggccggca gcagcaccgg caagaccggc agcggcaccg cgaccggcgg
     4801 cagcaccggc aaggccggca gcccggcggg caacgccact cccggcccgg ccaccggcgg
     4861 cgcggcagcg ggcaacgcag gcgcgggcgc cgcgaccggg ggcgccgcga ccggcgcgca
     4921 aggcaccgcg gcccaaggca gcgcgggcgg agcccccgcg gcggccgcca cccccgtggc
     4981 gcccacgccg gtcgaactcc aggatgtcgc cacggtcgaa ctgaccaact cggcggcctc
     5041 gaccgtcagc cgcagcaacg gcaagcctag tatcgccctc atcgtctaca agacgcagaa
     5101 cgcgaacacc gtccaagcc
//
LOCUS       DASUYC010000802         5838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_108513,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000802 DASUYC010000000
VERSION     DASUYC010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5838
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108513"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VF276_13150"
     CDS             <1..319
                     /locus_tag="VF276_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607848.1"
                     /translation="IRTAIFTDRSGNVVKELDNYQDNGYFQANGITVNYSEAGPNHVT
                     YYADGSVLYSGSGLFYNYRGPEYRFAGRVATLVDADGNVISDMFAGIDRGSIEDLCAA
                     LTP"
     gene            complement(470..1642)
                     /locus_tag="VF276_13155"
     CDS             complement(470..1642)
                     /locus_tag="VF276_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020543115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HEX6607849.1"
                     /translation="MAAVFREEAGRLTAALVRILGDFAVAEELVQDALLAALERWPVD
                     GIPARPAAWLWTVARRRAFNQLRRDARYRARLEQLAQPVPREPDDRLRLMFTCCHPAL
                     SRAAQVALTLRAVGGFTTGEIAHAFLASEAAVAQRIVRARRKIVAAGIPFRLPDDDAL
                     EDRVGEVLAVLYLMFTEGYLTSGGPVATRRDLAEDAAWLAALLVRLLPGEPEPLGLLA
                     LMRLHLARSAARFGGDGALVLLADQDRALWDQAQIVEGIALIEQAAALGRPGSYQVEA
                     AIAACHAEAPSWAATDWPQILLLYDLLLDLRPSPVVRLNRAVALRQVAGPEAALAEVT
                     ALADTLDHYHLFHAIRGELLLELGRREQARAADLRALALTNNHAEQTLLTRRLLEW"
     gene            complement(1669..2013)
                     /locus_tag="VF276_13160"
     CDS             complement(1669..2013)
                     /locus_tag="VF276_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014349894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX6607850.1"
                     /translation="MKYILLFCGTREQQQAWEDMTEEARAQQYEQVGRWFAENRAKLG
                     PSNQLQPPQTATTVRFQDSGEPLVHDGAFIEGNEVIGGYAEITVADLDEALHMAKTWP
                     ARGTVEIRPVME"
     gene            complement(2143..2430)
                     /locus_tag="VF276_13165"
     CDS             complement(2143..2430)
                     /locus_tag="VF276_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607851.1"
                     /translation="MRMLAPTLHYDFALVAEMAETLETFKVVPAEVLLLGGGKSPAWL
                     KAALDALMTVLPHAQRVTFPELDHGGSSDPSATNRGGRPAVVAEALRRFFA"
     gene            2499..2660
                     /locus_tag="VF276_13170"
     CDS             2499..2660
                     /locus_tag="VF276_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607852.1"
                     /translation="MAALPATEYPSVVALADHLLAPDADERFQFGIEILRHGLETRLE
                     RRGRPGAAK"
     gene            2759..3520
                     /locus_tag="VF276_13175"
     CDS             2759..3520
                     /locus_tag="VF276_13175"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX6607853.1"
                     /translation="MEHLVAFVHMLAPADDPLGAIYGLPAIFLVMLLKEAGVPIPVPA
                     DLIMLGAAARAAAGQLDLFGVIITFEVAMLIGGVIQYALAHGPGRGVLYRFGRYIGLT
                     GPRLDHAGSALQRGGVTALSVALTTPGVRAATVAAAGIAGLPLRVFLPGLFIGNTVFF
                     LLHVVIGYAGGQGIAGLLAATGLPAGPALIGLLVILAVIGFAGWMIIRRRARRAGAPP
                     PRLGEVAGAWEEACCPACLALGALGEARRPAAFAH"
     gene            3563..4903
                     /locus_tag="VF276_13180"
     CDS             3563..4903
                     /locus_tag="VF276_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013487883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX6607854.1"
                     /translation="MTLTSDPTIYQRPAEIAQALIRFDTTNPPGNEAACIGYIQQLLA
                     DAGIPVTILARDPARPNLIARLPGQGTAPPLLLQGHIDVVNTANQPWQHPPFAGEVID
                     GYLWGRGALDMKGGVAMMLAAMLRARAEGQALAGDVVLACVSDEENFGEYGAGFLVAE
                     HPDLFAGIRYALGEFGGFPFYLGGRRFYPIMVAEKQSCRVTATVRGPAGHGSRPMRGG
                     AMARLARMLTTLDTHRLPVHITPVGRLMIEEMAAALPEPLNAVLPALLDPAQTDALLD
                     RMGPEALGFDPLLHNTVNATIVHGGTQTNVIPSKIVVEMDGRTLPGFSGADLQAELVA
                     LLGDEVEIEVQAEPPAAATPDMGLFPTLSAILREADPEALPIPYMLPAVTDGRYFTRL
                     GIQTYGFLPMNLPEDFDFYASIHAADERVPVAALDFGADAIYKVLQRFGAAENR"
     gene            4900..5736
                     /locus_tag="VF276_13185"
     CDS             4900..5736
                     /locus_tag="VF276_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6607855.1"
                     /translation="MITGIDHIVILVTDLDQAQADYAAAGFTVVPGGEHAGGLTHNAL
                     VAFADGTYLELLAFRRPLSELAPGMPLPPGGLSRLTERWIGRAAGGPGLLDFALLPGT
                     MAADLDSARARGLALEGPRPGGRRLPDGQDVAWELGIPEAPELPFLCADVTPRDLRVP
                     TGPARDHANGALGVAGITVAVPALAPAAAHYTALLGAAPTGDDAAAPGAARATFLLGA
                     AEITLLTPDSPDDPLGPYLAAGGARPYTLQLRARPGAPALDAARLGGARISFLGAEDS
                     AA"
BASE COUNT          855 a         2119 c         1998 g          866 t
ORIGIN      
        1 gatccgcacc gccatcttca ccgaccgcag cggcaacgtg gtcaaggaac tcgacaacta
       61 ccaggacaac ggctatttcc aagccaacgg catcacggtc aattactccg aggccggccc
      121 caatcatgtg acctactacg cggacggcag cgtgctgtac tccggcagcg gcctgtttta
      181 caactaccgc gggcctgagt accggttcgc cggccgcgtt gccacgctgg tcgatgccga
      241 cggcaacgtg atctccgata tgttcgccgg gatcgaccgg ggatcgatcg aggacctttg
      301 cgccgcgctg actccgtaga gcagccggca ggggcgcctg gcaatactgg gcaaggacaa
      361 ccagaccaga ggggcagcag cagcgtgccg ctgctgcccc tctatctgcc gatcccggtg
      421 agcgatgggc ctgtcgagag ccgagttctc ttccgccgcc gggcgcgtct caccattcca
      481 gcagccgccg ggtgagcagg gtctgttcgg cgtggttgtt cgtcagcgcc agggcgcgca
      541 agtccgccgc gcgggcttgc tcgcgccggc ccagttccag cagcaactcg ccgcgaatcg
      601 cgtggaagag atggtagtga tccagcgtat cggcaagggc ggtcacctcg gccagcgccg
      661 cttcgggccc ggccacctgc cgcagggcca ccgcgcggtt gaggcgcacg accggggaag
      721 gccgtaggtc cagcagcagg tcgtagagca gcaggatctg cggccagtcg gtcgccgccc
      781 acgagggcgc ttcggcatgg caggccgcga tcgcggcctc cacctggtac gaccctggcc
      841 gcccgagcgc cgccgcctgc tcgatcagcg cgatgccctc gacgatctgg gcctggtccc
      901 acagcgcgcg atcctggtcg gccagcagga ccagggcgcc gtcgccgccg aatcgggcgg
      961 cgctacgggc gaggtgcagg cgcatgaggg ccagcaaacc cagcggttcc ggctcccccg
     1021 gcagcagccg gacgagcagc gccgccagcc acgcggcatc ttccgccaga tcgcgccggg
     1081 tggcgaccgg ccccccgctg gtcaggtagc cctcggtgaa catcaggtag agcaccgcca
     1141 gcacctcgcc cacgcggtcc tccagcgcgt cgtcgtccgg caggcggaag gggatgcccg
     1201 ccgcgacgat tttgcggcgc gcccgcacga tgcgctgggc cacggcggcc tcggaggcga
     1261 ggaacgcgtg cgcgatctcg ccggtggtga agccgccgac ggcccgcaac gtgagcgcga
     1321 cctgcgccgc ccgcgagagc gccgggtggc agcaggtgaa catcagccgc agccgatcat
     1381 cgggttcgcg cggcaccggc tgcgcgagct gttccagcct ggcccgatag cgtgcatcgc
     1441 ggcgcagttg attgaaggcg cgccgccggg ccaccgtcca cagccaggcg gcgggccgcg
     1501 cggggatgcc atccaccggc cagcgttcca gcgccgccag caacgcatcc tggacgagtt
     1561 cctcggccac ggcgaagtcg cccaggatac gcaccagggc cgcggtgagc cgccctgcct
     1621 cctcacggaa gacagcggcc accgcggcat gcgcctcggg cgggaccgtc attccatgac
     1681 cgggcggatt tcgacggtcc cgcgcgccgg ccaggtcttc gccatgtgca acgcctcatc
     1741 caggtcggcc acggtaatct cggcatagcc gccgatgacc tcattgccct cgatgaaggc
     1801 gccgtcatgg acaagcggtt cgcctgagtc ctggaagcgc accgttgtcg ccgtttgcgg
     1861 cggctggagt tggttgctcg gccccagttt cgcccggttc tccgcgaacc agcgccccac
     1921 ctgctcgtat tgctgcgcgc gggcctcctc ggtcatatcc tcccaggctt gctgctgctc
     1981 gcgggtgccg cagaacaaaa ggatatactt cattcgtcga ttcctccgct ttcttgcacg
     2041 atacaaacgg gcgcccccgg cggacaccct tctatatctt acgacaaaca gaaagcgcgc
     2101 aaatcgacag ccccgcggcc cgcccggcta gagattggtg catcaggcga agaaccggcg
     2161 cagcgcctcg gccacgacgg cgggccggcc gccgcgattg gtcgcactcg gatcgctcga
     2221 cccgccgtgg tcgagttcgg ggaacgtcac ccgctgggcg tggggcaaga ccgtcatcag
     2281 cgcatccagg gccgccttca accaggccgg gcttttgccc ccgccgagta acagcacctc
     2341 ggcgggcacc accttgaacg tctccagcgt ctccgccatc tcggcgacca gcgcgaagtc
     2401 gtaatgcaac gtcggcgcca gcatgcgcat cgtgacatcg cctagcttcg cggtcttatc
     2461 ctcacgcgga agcggcaacc gccggggcgc agaactggtt ggccgcgttg ccggccaccg
     2521 agtatcccag cgtggtcgcc ctcgcggatc atctgctggc accggacgct gatgagcggt
     2581 tccagttcgg catcgagatc ctgcgccacg ggctggaaac gcggctggag cgccggggcc
     2641 gccccggcgc ggcaaagtga gcagcatttg ggctagacaa tgagcagggc gacagcatac
     2701 aataggcgta ccgagacaaa cagggagcgg gaccgcttgt agtagacagg gagccgccat
     2761 ggaacatctc gtcgcctttg tgcatatgct cgcccccgcg gatgacccgc tgggcgcgat
     2821 ttacgggctg ccggctattt tcctcgtcat gctgctcaag gaggccgggg tgccgatccc
     2881 ggtgcccgcc gacctgatca tgctgggcgc ggcggcgcgg gccgccgccg ggcaactcga
     2941 cctgttcggc gtgatcatca cgttcgaggt ggcgatgctg atcggcgggg tgatccagta
     3001 cgcgctggcc cacgggccgg ggcgcggggt actctaccgc ttcgggcgct acatcggcct
     3061 gaccgggccg cggctcgacc acgccgggag cgccttgcag cgcggcggcg tgaccgcgct
     3121 cagcgtggcg ctgaccacgc cgggtgtgcg cgcggccacc gtggccgccg ccgggatcgc
     3181 cgggctgccg ttgcgcgtct tcctgcccgg tctgttcatc ggcaacacgg tcttctttct
     3241 gctgcatgtc gtcatcggct acgccggggg ccagggcatc gccgggctgc tggcggccac
     3301 cggcctgccg gcaggtcccg cgctgatcgg cttgctcgtg atcctggccg tgatcggctt
     3361 cgccgggtgg atgatcatcc ggcgtcgcgc ccgccgcgcc ggagcgccgc cgccgcgcct
     3421 gggcgaagtg gcgggcgcct gggaagaagc ctgctgcccg gcctgcctgg ccctgggcgc
     3481 gctgggcgag gcgcgccgcc ccgcggcctt cgcccactga ccgcccatac ctgcgaccta
     3541 tcgttgagga gtgccgccct ttatgaccct gaccagcgac ccgacgatct accaacgccc
     3601 cgccgaaatc gcgcaggcgc ttattcgctt cgacaccact aatccgcccg gcaacgaggc
     3661 cgcctgtatc ggctatatcc agcaactgct ggcggacgcc ggcatcccgg tgacgatcct
     3721 ggcccgcgac ccggcccggc ccaacctgat cgcccggctg cccggccagg gcacggcccc
     3781 gccgctgctc ttgcaggggc atatcgacgt ggtgaacacg gccaaccagc cctggcagca
     3841 cccgcccttc gccggggagg tgatcgacgg ctacctctgg gggcgcggcg cgctcgatat
     3901 gaagggcggc gtcgccatga tgctggcggc catgctgcgc gcccgcgccg agggccaggc
     3961 cctggcgggc gatgtggtgc tggcctgcgt cagcgacgag gagaatttcg gcgagtacgg
     4021 cgcgggcttc ctggtggccg agcaccccga cctgttcgcg ggcatccgct atgcgctggg
     4081 cgagttcggc ggtttcccct tctacctggg tgggcggcgc ttctacccga tcatggtggc
     4141 cgagaaacag tcgtgccggg tgacggccac cgtgcgcggg ccggcggggc acggctcgcg
     4201 gcctatgcgc ggcggcgcca tggcccgcct cgcccgcatg ctcaccacgc tggacacgca
     4261 ccggctgccg gtccatatca cccccgtcgg gcgcctgatg atcgaagaga tggcggcggc
     4321 gctgccggag cccctgaacg ccgtgctgcc cgcgctgctc gacccggcgc agaccgacgc
     4381 cctgctcgac cgcatggggc cggaggcgct gggctttgac ccgttgctgc acaacacggt
     4441 caacgcgacc atcgtgcacg gcggcacgca gaccaacgtc atccccagca agatcgtggt
     4501 cgagatggac gggcgcacgc tgcccggctt cagcggggcc gacctgcaag ccgaactggt
     4561 cgcgctgctg ggcgacgagg tggagatcga ggtgcaggcc gaaccgcccg ccgcggccac
     4621 gcccgacatg ggcctgttcc cgaccctcag cgccatcctg cgcgaggccg acccggaagc
     4681 cctgccgatc ccctacatgt tgcccgccgt caccgacggg cgctatttta cgcgcctggg
     4741 catccagacc tacggcttcc tgccgatgaa cctgccggag gacttcgact tctacgccag
     4801 catccacgcc gccgacgagc gcgtgcccgt ggcagcgctg gacttcggtg cggacgccat
     4861 ctacaaggtc ttgcaacgct tcggcgcggc ggagaaccga tgattacggg catcgatcac
     4921 atcgtcatcc tggtcacgga cctggatcaa gcacaggccg attacgcggc ggcagggttc
     4981 accgtggtgc cgggcggcga acatgccggc gggctcacgc acaacgccct ggtcgccttc
     5041 gccgacggca cctacctgga gttgctggcc ttccggcgcc ccctgagcga actggcgccc
     5101 ggtatgccgc tgccgcccgg cgggctgagt cgcctgacgg agcgctggat cggccgcgcg
     5161 gcgggcgggc cgggcctgct cgatttcgcc ctgctgcccg gcaccatggc tgccgacctg
     5221 gactctgcca gggcgcgcgg gctggccctg gaggggccac gccccggcgg gcggcgcctc
     5281 cccgacggcc aggacgtggc ctgggaactc ggcatcccgg aggcgccgga actgcccttc
     5341 ctgtgcgccg acgtgacccc gcgcgacctg cgcgtgccca cgggccccgc gcgcgaccat
     5401 gccaacggcg cgctcggcgt ggccggcatc accgtggcgg ttccggcgct ggccccggcg
     5461 gcagcgcatt atacggcgct gctcggcgcg gcgcccaccg gcgacgacgc ggcggccccc
     5521 ggcgcggcgc gcgccacctt cctgctgggc gcggcggaga tcacgctgct gacccccgac
     5581 agccccgacg acccgctggg cccttacctg gcggcgggcg gcgcgcggcc ctataccttg
     5641 caactccggg cacggcccgg cgccccggcg ttggacgcgg cgcgcctggg cggcgcacgg
     5701 atcagttttc ttggcgcgga ggatagcgca gcatgaacgc gaatcactat cacctggtca
     5761 cccactggca cgtgcggggc cgggtggaag agatttatga cctgctctac gatgcgaccg
     5821 acctgccccg ctggtggc
//
LOCUS       DASUYC010000803         2963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_587490,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000803 DASUYC010000000
VERSION     DASUYC010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587490"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1287
                     /locus_tag="VF276_13190"
     CDS             <1..1287
                     /locus_tag="VF276_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607856.1"
                     /translation="TLGTIAQVRAGEIRPGAYSYARVTAPTDYPPGTLLVLWAVGKLY
                     QAAVAPDLADSPTFQVLIKLVVVLGDALGAALIAWAVRRWAGPPGGTRLAAVAGLAYW
                     CNPTVIVAGSVLGYLDALYVLPLLALLAALTLRRYAVGWVAWGVAFMVKLQALFVAPA
                     LGLLSLRRGPARLLGYGGAALGTIGLFCAPFLLKGTFVGVLAGIGNNAQEGYLSANQV
                     NLWWVWSYFWEWNAEGKGPGVLVRIVPVSVVSEAHVADLNAWTIGLFAAFLLGTTAVW
                     AWGNRGRAPAAPRVTDLFLVPLHFYGATMLLPSIHENHLLPVPALVLLLAGLAGARGA
                     QPLARQLAGLYVALSVIVGLNLVLFYGFGRGALAPVPRMWFGADASVGLAALNVVLFA
                     VTLAVWGVAHVRTGGPRSAPADPGGRASVVTAVDRA"
     gene            complement(1346..>2963)
                     /locus_tag="VF276_13195"
     CDS             complement(1346..>2963)
                     /locus_tag="VF276_13195"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX6607857.1"
                     /translation="FASGIPTVRFLSYSPAGDLYAGQLEGRNSAITALPDRDHNGQAD
                     RAIQVATGLYSPNNVTFRPVGFGTVFATGALDQVRVYTDTRGDLSFATSRLLVGNLSN
                     VTSGRHKTKTVLYGPDGLLYLSKGSFDDDPTHADPNAGIWRYNPDGSGEQKVASGLRN
                     AVGLAWDAVGGALWADDNGSDDQGRNQPHDELDQIVVGGDYGWPFCIDNQVHYAGAPA
                     YDCSQTRAPAVLLAPHAGALGMAFYSGGTFPARYWGGLFVAYHSIQYPEQRGVYFIPF
                     TDGKPSGGPELFMGGTNNRWMGLAINPYDGSLMVSDDREGRIFQVVYMGPPPAPIPPA
                     TAPPAAPGKPQPVMAAPLPGFARRFPETNHTLNGAFLQFWFWNGGLARYGFPISDPMT
                     EKLADGKTYTVQYTQRARLEYHPENRGSPYEVLLGRLGADLVAGRTEPAFQPAPPQPG
                     AQFVTETRHNLSGPIAGYWRRNGGVPVFGYPLSEVFTEVSPTDGKPYQVQYFERNRLE
                     YHPENAGTPNEVLLGLLGVQSYQARYGPLP"
BASE COUNT          343 a          994 c         1132 g          494 t
ORIGIN      
        1 acgctcggca ccatcgccca ggtgcgcgcg ggcgagatcc gccccggtgc ctactcctat
       61 gctcgcgtca cggctcccac cgactacccg cccggcaccc tgctggtgct ctgggccgtg
      121 ggcaagctct accaggccgc cgtcgccccc gatctcgccg acagccccac gttccaggtc
      181 ttgatcaagc tggtggtcgt gctgggcgac gcgctgggcg ccgcgctgat cgcctgggct
      241 gtgcggcgct gggccgggcc gcccggtggc acccggttgg ccgcggtggc cgggctggcc
      301 tactggtgca accccacggt tatcgtcgcc gggtcggtgc tgggctacct cgacgcgctc
      361 tacgtgctgc cgctgctggc gctgctggcg gcgctaaccc tgcggcgcta cgcggtgggc
      421 tgggtggcct ggggcgtggc cttcatggtg aagttgcagg cgctgttcgt ggcgccggcg
      481 ctgggcctgc tcagcttgcg gcgcggcccg gcccggctgc tgggctacgg tggggcggcg
      541 ctcggcacga tcgggttgtt ctgcgcgccg ttcctgctta agggcacctt cgtcggcgtg
      601 ctggccggca tcggcaacaa cgcccaggag ggctacctct cggccaacca ggtcaatctc
      661 tggtgggtct ggtcgtactt ctgggagtgg aacgccgagg gcaaggggcc gggcgtgctg
      721 gtgcgcatcg tgcccgtctc cgtcgtgagc gaggcccatg tcgccgacct caacgcctgg
      781 accatcggcc tgttcgcggc gtttctgctg ggcacgacgg cggtctgggc ctggggcaac
      841 cgggggcgcg cgcccgcggc gccgcgcgtg accgacctgt tcctggtgcc gctgcacttc
      901 tacggcgcga ccatgctgct gccgtcgatc cacgaaaacc acctgctgcc ggtgccggcg
      961 ctggtgctgc tgctggccgg gctggccggc gcgcggggcg cccagcccct ggcgcggcaa
     1021 ctggccggcc tctacgtggc gctctcggtc atcgtgggcc tcaacctggt gctgttctac
     1081 ggctttggcc ggggcgccct cgcgccggtg ccgcgcatgt ggttcggcgc cgacgcctcg
     1141 gtcgggctgg ccgcgctcaa cgtcgtgctg ttcgccgtga cgctagccgt gtggggcgtg
     1201 gcccatgtcc gcaccggtgg gccgcgcagt gcccccgctg accccggcgg gcgggcgtcc
     1261 gtcgtgacgg cggtagatcg cgcgtagcga cgcccggccc cgttgcccgg cccttgcaag
     1321 ggccgggcaa cccgcgcggc gtggcttacg gcagcgggcc gtagcgcgcc tggtagctct
     1381 gcacgccgag caggcccagc agcacctcgt tgggcgtgcc cgcgttctcc ggatggtact
     1441 ccaggcggtt gcgctcgaag tattgcacct ggtagggctt gccgtcggtc gggctgacct
     1501 cggtgaacac ctcgctcagc gggtagccga acaccggcac gccgccgttg cgccgccagt
     1561 agccggcaat gggcccgctc aagttgtggc gcgtctcggt gacgaactgc gcgccgggct
     1621 gcgggggggc gggctggaag gccggttccg tgcgcccggc caccagatcg gcgcccaggc
     1681 ggcccagcag cacctcatag ggcgagccgc ggttctccgg gtggtattcg agccgggcgc
     1741 gctgcgtgta ttgtacggtg taggtcttgc cgtcggccag cttttcggtc atcgggtcgc
     1801 tgatcgggaa gccgtagcgg gccaggccgc cgttccagaa ccagaattgc aggaacgcac
     1861 cgttgagcgt gtggttggtc tcggggaagc ggcgggcgaa gccgggcagc ggcgcggcca
     1921 tgaccggctg cggcttgccg ggcgcggcgg gcggcgcggt ggccggcggg atgggcgcgg
     1981 gcggcggccc catgtagacc acctggaaga tgcgcccctc gcggtcgtcc gacaccatca
     2041 gcgagccgtc atacgggttg atcgccagcc ccatccagcg gttgttggtg ccgcccatga
     2101 acagttcggg tccgcccgag ggcttgccgt cggtgaaggg gatgaagtag acgccgcgct
     2161 gctccgggta ctggatgctg tgataggcga cgaacaggcc gccccagtag cgcgcgggga
     2221 aggtgccgcc gctgtagaag gccatgccga gcgcgcctgc atggggggcc agcagcacgg
     2281 cgggcgcgcg ggtctggctg caatcatagg ccggggcgcc cgcgtagtgg acctggttgt
     2341 cgatgcagaa gggccagccg tagtcgccgc cgaccacgat ctggtcgagt tcgtcgtgcg
     2401 gctggttgcg gccctggtcg tccgagccgt tgtcgtcggc ccagagcgcg ccgcccacgg
     2461 cgtcccaggc caggcccacg gcgttgcgga ggccggaggc cactttctgc tcgccgctgc
     2521 cgtcggggtt gtagcgccag atgcccgcgt tggggtcggc gtgagtcggg tcgtcgtcga
     2581 acgagccttt ggacaggtag agcaggccgt cggggccgta gagcacggtc ttggtcttgt
     2641 gccgcccgct ggtcacgttc gacaggttgc ctaccagcag cctggacgtg gcgaacgaga
     2701 gatcgccgcg cgtgtcggtg tagacgcgca cctggtcgag cgcgccggtg gcgaacacgg
     2761 tgccgaagcc cacgggccgg aaagtgacgt tgttgggcga gtagaggccc gtcgccacct
     2821 ggatggcgcg gtcggcctgc ccgttatggt cgcggtcggg cagcgcggtg atggcgctgt
     2881 tgcggccctc cagttgcccg gcatacaggt cgcccgccgg gctatagctg aggaagcgca
     2941 ccgtcgggat gcccgacgcg aag
//
LOCUS       DASUYC010000804         2949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_851203,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000804 DASUYC010000000
VERSION     DASUYC010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851203"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VF276_13200"
     CDS             <1..789
                     /locus_tag="VF276_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607858.1"
                     /translation="AEVPAARPVLLELYEYYDTDGVKRDPGCQIRSRILQALLPVSVA
                     PDAALAERALTTYEFLPPTRAESAGGLRAAGLALLAEWDPGPATYHAVRLLTDLHTAR
                     MSGEPAVTAARLLGGQGELLPLYGYVLDLAHAISEVWAECLRHLAGTPAAVLTDLLAR
                     YGATDDEFVLIGVLDLLLAHEARASQRAFLTDFLRTTRRYAVYRYLVTSIVARRAPEE
                     QALLLDVAAEERDPRKIALLLEALALAPPDPAVATAIAGLERRR"
     gene            complement(947..1543)
                     /locus_tag="VF276_13205"
     CDS             complement(947..1543)
                     /locus_tag="VF276_13205"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX6607859.1"
                     /translation="MMQLDVPQAASGAAGPEGQVAPHTITLSDGTAIVVRGICPEDAP
                     ALRRFHSRLSAHSIYNRFFAWLPELSEERAEYFTHLDPAYRQALVAQDPDQPEELIGV
                     IRYDRTPGTDRAEYAAVLADRWQGRGLGRRMTRELIDTAWRQGIRRLDAYILPDNRPM
                     RQLLARMDLPMTLRWEDEMIHVELDLNEIQHPDSYPIG"
     gene            1952..2821
                     /locus_tag="VF276_13210"
     CDS             1952..2821
                     /locus_tag="VF276_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015158308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX6607860.1"
                     /translation="MQDIGLTALAPLIWGTTYIVTTEFLPPDRPLLAGLMRALPIGLL
                     LLLVFRRLPAGVWWGRTLVLGALNIGLFFPLFFLGVYRLPGGVAATLGAFQPFLVALF
                     GWPLLGLRPGRRTMLAAGAGLLGIAMLVLGPAARLDALGVVAGLGGAALMALATVLGK
                     RWGRPAPVLVFTAWQLVVGGLMLVPLTLAFEGPPPPLTGANWLGYVYLGLINTGLAYA
                     LWFRGVVRLPATAITFLMLLSPVVAVILGFVVLGQTLSMVQIAGALLVLGSVIAGQRP
                     GTRAVPAGAAAIS"
BASE COUNT          363 a          989 c         1082 g          515 t
ORIGIN      
        1 gccgaggtgc cggcggcccg gcccgtgctg ctggagctgt acgagtatta tgacaccgat
       61 ggggtgaagc gcgatcccgg ctgccagatc cgcagccgca tcctccaggc gctgttgccg
      121 gtcagcgtgg cgcccgatgc cgccctggcc gagcgcgccc tgaccaccta cgagtttctg
      181 ccgccgacgc gggccgagag cgccgggggc ttgcgcgcgg caggcctggc cctgctcgcc
      241 gaatgggacc ccggcccggc cacctatcac gccgtgcggc tgctgaccga cctgcacacg
      301 gcgcgcatgt cgggcgaacc ggcggtgacg gcggcccgct tgctgggcgg ccagggcgaa
      361 ctgctgccgc tctatggcta cgtgctggat ctcgcccacg ccatctccga ggtctgggca
      421 gagtgcttgc gccacctggc gggcaccccg gcggcggtgc tgaccgatct gctggcgcgc
      481 tacggcgcca ccgacgatga gtttgtgctg atcggcgtgc tcgacttgct gctggcgcac
      541 gaggcccgcg cgagccagcg cgcctttctc accgacttcc tgcgcaccac gcgccgttat
      601 gccgtgtacc gctacctggt gacgagcatc gtggcgcggc gggcccccga ggagcaggcc
      661 ctgctgctcg acgtggccgc cgaggagcgc gacccgcgca aaatcgcgtt gctgctggag
      721 gcgctggccc tggccccgcc cgatcccgcg gtcgccacgg ccatcgcggg cctggagcgg
      781 cggcggtgat tgggccgcgg gccggtttca aaccggcccc tacagcggca ggggggcgga
      841 gcaaaccaca gggcgatgga tgcggaaacg ttgcgggttg attcgtcgcg gcgggcggcg
      901 cggggcgccg ggtgccattc atcgcacccg gtgccccgcc ggcatctcag ccgatggggt
      961 agctgtcggg gtgctggatt tcgttaagat cgagttccac gtggatcatc tcgtcttccc
     1021 agcgcagggt catcggcagg tccatgcggg ccagcaactg gcgcatcggg cggttgtcgg
     1081 gcaggatata ggcatcgagc cggcggatgc cctgccgcca ggcggtgtcg atcaactcgc
     1141 gggtcatgcg ccggcccagg ccacgcccct gccagcggtc ggccagcacg gcggcatatt
     1201 ccgcccggtc ggtgccgggc gtgcgatcat agcggatgac gccgatgagt tcctccggct
     1261 ggtcgggatc ctgggccacc agcgcctgcc gataggcggg atcgagatgg gtgaaatact
     1321 cggcgcgttc ctccgagagt tcgggcagcc aggcgaaaaa gcggttgtag atcgaatggg
     1381 cgctgagccg gctgtggaag cggcgcaggg ccggggcatc ctccgggcaa ataccgcgca
     1441 cgacaatggc ggtgccgtcc gagagggtaa tggtgtgggg ggccacctgg ccctccggcc
     1501 cggcggctcc gctcgcggct tgcgggacgt cgagttgcat cattgtcggt tctcccgcgc
     1561 acccggcgcc caatcgccgg aacccaggga tggccgggtc tcgcggctgg gccggtgcgc
     1621 ctcgctcacg ctaatgagcg taatggtagt gcgcgggcct ggccggtttc aaaccggccc
     1681 ctacacctac gatcccggcg ccgtctacca tggctgctcg ccgcggggcg cggttcggcg
     1741 tgatctaatg gcgtcccgtc gagctagtga aagtcgtaac gatccacttt cattataacg
     1801 cttattcgcg cgcaatgtat ccgccgaaag gccctgatca tggtgggacg gttggcccat
     1861 ggtcgcgccc ccaccggcgc ggtttatgct tttaggctag agggccggga ttgcgcggca
     1921 tcgctgcaca aggatggagg agtatcgcgg gatgcaggat attgggctga cggcgctggc
     1981 gcccctgatc tgggggacca cctatattgt caccacggag tttttgccgc ccgaccggcc
     2041 gctgctggcc gggctgatgc gggcgctgcc gatcgggcta ttgctgctgc tggtcttccg
     2101 gcgcttgccc gcgggcgtgt ggtgggggcg gacgctggtg ctgggcgcgc tgaacatcgg
     2161 cctgtttttc cccctgttct tcctgggcgt ttaccggctg ccgggcgggg tggcagccac
     2221 gctcggcgcg ttccagccct tcctggtggc gctgttcggc tggccgctgc tggggctgcg
     2281 accgggccgg cgcacgatgc tggccgccgg ggcgggcctg ctgggcatcg ccatgctggt
     2341 gctggggccg gcggcccggc tcgacgcgct gggcgtcgtc gcggggttgg gcggcgccgc
     2401 gctgatggcg ctcgctaccg tgctgggcaa gcgttggggc cgccccgcgc cggtgctggt
     2461 gttcaccgcc tggcaactgg tggtcggcgg gctgatgctc gtgccgctga cgctcgcgtt
     2521 cgaggggccg ccgcccccgc tgaccggcgc caactggctc gggtacgtct acctggggct
     2581 gatcaacacg ggcctggcct acgccctctg gttccgcggc gtggtgcggc tgcccgccac
     2641 ggcgatcacc tttctgatgc tgctcagccc ggtcgtcgcc gtcatcctgg gcttcgtggt
     2701 cctggggcag acgctgagca tggtgcagat cgccggggcg ctgctggtgc tgggcagcgt
     2761 catcgccggg cagcgccccg gcacccgcgc ggtgccggcc ggggccgccg ctatctcctg
     2821 acctgttgcg ccggttcgcc tggctagcca ccggtctcta atacacgccc acgttgattt
     2881 tgcggtgtag gggccggttt caaaccggcc cggccccggc agaagcagta cgctgcacga
     2941 cccttgcta
//
LOCUS       DASUYC010000805         4915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1127105, whole genome shotgun sequence.
ACCESSION   DASUYC010000805 DASUYC010000000
VERSION     DASUYC010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4915
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127105"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3137)
                     /locus_tag="VF276_13215"
     CDS             complement(<1..3137)
                     /locus_tag="VF276_13215"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX6607861.1"
                     /translation="MSKRRASRSAAAEFSPGEDYPARNGLSALESALAAAETVAPPPP
                     ADLGWLVRLSQELAGLREPDAVAAAAAAIISAALDARVAIWQGADAEAQDLAALSGAP
                     GTIAATAPPAEASAAPARPLLLLASAGFGQAFARLYGRGAGHGAPAAAVDPAASREAP
                     AARAWATLAPVLVGDPSAEQAGPDALPETWRNMARREGIGGGWALPLLAGADAPVGVL
                     ECYLPAAVADDSPLRDLLRVAAGQLGFALGQTGVQEKMGRRLAEALLLREVSQMIGNS
                     IDLEDTLAAVLASVRRLIPYSSSEITLYDSDKHRLVTAAVAGEDIFVGRHHAEYGEGE
                     GYTGWIAQQRRPLVVPDVEARTDVQPKTKVLPGGMVMRAYIGLPMVTLVAGGDGDRLL
                     GTLEVVFDQPDAFALHDVRLLQQVADEAAVAIENAQVYRSSRERLDRRLQELMVLQRI
                     GRELNSTWDLERIFELLAREAVQATGARSAGIIQVDHEGGDYVWTTVYGLDPDRESAH
                     VGVPMPIARGLVGRAITTGQAQMVGNVHQDPDYYEVDPAIRSELVMPIRYEDQVVGVL
                     NLESTEYEAFDEDALRFVEALADQAAIAVGNAQNYEAQVRQGEQLLRRASQLSQVLEI
                     SNALTGEQPLPEILDQIVHAVVETAGFRTALLSMVDPAQPDVLERAAAAGLPLPVFRD
                     LRQHPSSVRDVTEGVLRPEFRIGRAYYISHEHRDVWHARIQSVTMMDSDAAYEPGGWH
                     PDDALLLPLTSTMGEFLGLLSVDDPIDGRRPTRAVVETLEIFANQAAIAIENAQLFAR
                     YKHRITELGTLNQLGQRLASSLTIDAIGASIYEHLRQFLPVDAFYIALYDRDRQMLDF
                     PYNVDLGDVIVLTPNRFGEGMSSEVIGTGTALFVHDLSDPAQQPASYSGMTWGHGACR
                     TWVGVPMVLGQQVIGVLSAQSYQPNAFSTEQVQFLDTVANQAAIAIENARLFDRHKHR
                     ITELGTLNQLAQSLGALLNVEAIAVSVYEHLRQLMTVDSFYIATYDAAKGLIHFPYFI
                     DVDELLHIDPQPFGRG"
     gene            complement(3164..4093)
                     /locus_tag="VF276_13220"
     CDS             complement(3164..4093)
                     /locus_tag="VF276_13220"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6607862.1"
                     /translation="MAGTNHGKILIIDDTVDIAQMLNFYFQSQGFDTRMAHRGGDGIG
                     AARSWGPNLILLDINMPDMDGYDVARTLRQSLRTSHIPIMFLTERGEKSAKLAGLELG
                     VDDYITKPFDIDELHLRAVNALKRANQESLTNPVSGLPGSQLIEDQLKALLRKPTWAL
                     ATCTVNHFGPFNDVYGFVAGDDALKTIAMLLTDVLNEKGGDEDFLGHVGGAEFLLTTH
                     PDRVRALCEAAIVRFDRDMPLLYSYQDRKAGYMTIQEPSGKQRTVPLMSLSIGVITQA
                     QGPFTDIRELSEVAAEARQKARLAAGSSVYVER"
     gene            complement(4063..>4915)
                     /locus_tag="VF276_13225"
     CDS             complement(4063..>4915)
                     /locus_tag="VF276_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HEX6607863.1"
                     /translation="LKAFIPIESWVAVMLTNSLFGVLGWARRLLKESNSLKVKLNILT
                     RVVLERKHPLSCSAVVRIGRNCKIDPGVIIQGPTIIGNNVTIGAGSLIVSSVIGDNVN
                     IMQGAQIVLSMVSNGCYIPFRAALYGTSFMENTMVAQNTVLQGCVVGRNTFIGGGNTF
                     TDYNLLGQPIRTMHRGRLEPVGMAACGCAVGHDCRIGAGFVFYPGRTVESGTILIYRG
                     DQAVIDHNITADMHGQDPRIRRLYTADSLVINEDISSGTPDLAPPAAAELEKRGEVWQ
                     APTMAKY"
BASE COUNT          815 a         1616 c         1640 g          844 t
ORIGIN      
        1 ccacgcccaa acggctgcgg atcgatatgc agcagttcgt ccacgtcgat gaagtacggg
       61 aagtggatca gccccttggc cgcgtcatac gtcgcgatat agaaggagtc aaccgtcatc
      121 aactggcgca gatgctcgta gaccgagacg gcgatggcct cgacattcag cagcgcgccc
      181 aggctctgcg ccaactggtt cagcgtcccc agttcggtga tgcggtgctt gtggcgatcg
      241 aacagccggg cattctcaat ggcgatcgcg gcctggttgg ctaccgtgtc gaggaattgc
      301 acctgctcgg tgctgaaggc gttgggctgg tagctttgcg ccgacaacac gccgatgacc
      361 tgttgcccca gcaccatggg cacccccacc caggtgcggc aggcgccgtg gccccaggtc
      421 atgccgctgt acgacgccgg ctgctgggcc gggtcgctca ggtcgtggac gaacagcgcc
      481 gtcccggtgc cgatcacctc gctcgacatg ccctcgccaa aacgattcgg cgtgagcacg
      541 atcacgtcgc ccaggtccac gttatagggg aagtcgagca tctgccggtc gcgatcatat
      601 agggcgatgt agaaggcatc gaccggcagg aactggcgca gatgctcgta gatcgacgcg
      661 ccgatggcgt cgatggtcaa cgacgaggcc agccgctggc cgagctggtt caatgtgccg
      721 agttcggtga tgcggtgctt gtagcgggcg aagagttgtg cgttctcgat ggcgatggcc
      781 gcctggttag cgaagatttc cagtgtctcg accaccgccc gcgtgggccg gcgcccgtcg
      841 atgggatcgt ccaccgacag caagcccagg aactcgccca tggtgctggt cagcggcagc
      901 agcagggcat cgtccgggtg ccagccgccg ggctcatagg cggcgtcgct gtccatcatc
      961 gtcaccgact ggatgcgcgc atgccagaca tcgcggtgtt cgtggctgat atagtaggcg
     1021 cgcccgatgc ggaactcggg gcgcaacaca ccttcggtca cgtcgcgcac ggacgaggga
     1081 tgctggcgca gatcgcggaa caccggcagg ggcagccccg ccgccgcggc ccgttccagc
     1141 acatcaggct gcgcgggatc gaccatgctg agcagcgccg tgcggaagcc cgccgtctcc
     1201 accaccgcat ggacaatctg gtcgagaatc tcgggcagcg gctgctcgcc ggtcagcgcg
     1261 ttgctgattt cgagcacctg gctgagctgg ctggcccggc gcagcaactg ctcgccctgg
     1321 cgcacctggg cctcgtagtt ctgggcgttg cccacggcaa tagccgcctg gtcggccagt
     1381 gcctcgacaa agcgcagggc gtcctcgtcg aaggcttcgt attccgtgct ctccaggttc
     1441 aggacgccga ccacctggtc ctcatagcgg atcggcatca ccagttccga gcggatcgcg
     1501 ggatcaacct cgtaataatc ggggtcctgg tggacgttgc ccaccatttg ggcctgcccg
     1561 gtcgtaatcg cacgacccac taggccgcgc gcgatgggca tgggcacgcc gacatgcgcc
     1621 gactcgcggt ccgggtccag gccgtaaacc gtggtccaga cataatcgcc gccttcatgg
     1681 tcgacctgga tgatgccggc cgagcgggcg cccgtggcct gcacggcttc gcgcgccagc
     1741 agttcgaaga tgcgctccag gtcccaggtc gagttgagtt cgcgcccgat gcgctgcaac
     1801 accatcaact cttgcaggcg gcgatccaga cgttcgcgcg aactgcggta gacctgagcg
     1861 ttctcgatgg cgacggcggc ttcgtcggcc acctgctgca acaggcgcac gtcgtgcagg
     1921 gcgaaggcgt cgggctgatc gaagaccact tcgagcgtgc ccagcaggcg gtcgccgtcg
     1981 ccccccgcga ccagcgtcac cataggcagg ccgatatagg cgcgcatgac catgccgcca
     2041 ggcaggacct tcgtcttggg ctgcacatcg gtacgcgcct cgacatcggg caccaccagc
     2101 gggcggcgct gctgggcgat ccagccggtg tagccctcgc cttcgccgta ctcggcatgg
     2161 tgccggccca cgaaaatatc ctcgccggcc accgccgccg tgaccaggcg gtgcttatcg
     2221 gagtcataga gggtgatttc gctgctgcta taggggatga ggcggcgtac cgaggccagc
     2281 acggcggcca gcgtgtcttc caggtcgatc gagttgccaa tcatctggct cacttcgcgc
     2341 aacagcagcg cctcggccag gcggcggccc attttctcct gcacgccggt ctgccccagc
     2401 gcgaagccca actgcccggc ggcgacccgc agcaagtcgc gcagcgggga gtcgtccgcg
     2461 accgcggcgg gtaggtaaca ctccagcacg cccaccggcg catcggcccc ggccagcagc
     2521 ggcagcgccc acccgccgcc gatgccctcg cggcgggcca tgttgcgcca tgtctccggc
     2581 agcgcgtccg gcccggcctg ctctgcactc gggtcgccga ccagcacagg cgccagggtg
     2641 gcccaggcac gggccgctgg tgcctctcta gaggcagccg ggtccaccgc cgcggcgggc
     2701 gcgccatgcc cggccccacg accatacagc cgcgcgaacg cctgaccgaa gccggcgctc
     2761 gccagcagca gcaggggccg ggccggggcg gcgctagctt cggcgggcgg cgcggtggcg
     2821 gcgatggtcc ccggtgcgcc cgacagcgcc gccaggtcct gggcttcggc atccgcgcct
     2881 tgccagatgg ccacccgcgc atccagcgcc gcgctgatga tcgcggcggc ggcagcggcc
     2941 acggcgtccg gctcgcgcag ccccgccagt tcctggctga ggcgcaccag ccagccgaga
     3001 tccgccgggg gcggcggcgc tacggtttct gccgcggcga gagcggattc gagggcggag
     3061 aggccgttgc gggcggggta atcttcgccc ggcgagaact cggcggccgc ggagcgagaa
     3121 gcacgacgct tggacacgat taggatcctt ctgctacagt tggctagcgc tcgacgtaga
     3181 ccgagctacc ggcggccagg cgcgctttct gccgcgcttc cgcggccact tcactcagtt
     3241 cgcgaatatc ggtaaacggg ccttgcgcct gggtgatcac tccgatcgac aggctcatca
     3301 gcggcacagt acgttgcttg ccgctcggct cctggatggt catgtagccg gccttgcgat
     3361 cctggtagga atagagcaac ggcatatcgc ggtcgaagcg cacaatcgcg gcttcgcaca
     3421 gcgcgcgcac gcggtcgggg tgcgtggtca gcaggaattc ggccccgccg acgtggccca
     3481 ggaagtcctc gtcgccgccc ttttcgttca gcacgtcggt gagcagcatg gcgatggtct
     3541 tgagcgcgtc gtccccggcg acgaagccgt agacatcgtt gaacgggccg aagtggttga
     3601 ccgtgcaggt ggcgagcgcc cacgtgggct tgcgcagcag cgccttcaac tggtcctcga
     3661 tcaactgcga gccaggcagg cccgacaccg gattggtcag gctctcctgg ttggcgcgct
     3721 tgagtgcatt cacggcccgc aggtggagtt cgtcgatgtc gaacggcttg gtgatatagt
     3781 cgtccacgcc gagttcgagg ccggccagtt tggcgctttt ctcgccgcgc tcggtcagga
     3841 acatgatcgg gatgtgactg gtgcgcaggc tctggcgcag ggtgcgcgcg acatcgtagc
     3901 cgtccatatc gggcatgttg atgtcgagca ggatcaggtt gggcccccaa ctacgcgccg
     3961 ccccgatgcc gtcgccgccg cggtgggcca tgcgcgtgtc aaagccctgc gactggaagt
     4021 agaagttaag catctgggca atatcgacgg tatcgtcgat gatcagtatt ttgccatggt
     4081 tggtgcctgc catacctctc cccgtttctc tagctccgct gccgctggag gtgccaggtc
     4141 gggcgtgccg gagctgatgt cttcgttgat caccaggctg tccgccgtat acaggcggcg
     4201 gatgcgcggg tcctgcccat gcatatcggc ggtgatattg tggtcgatca ccgcctggtc
     4261 gccccggtag atcaggatgg tgccggactc gactgtacga ccagggtaaa acacaaatcc
     4321 ggcgccgata cggcaatcat ggcccacggc acagccgcag gccgccatac ccaccggttc
     4381 caaccgtccg cgatgcatcg tccggatcgg ttggcccagg agattgtaat cggtaaaagt
     4441 gttcccgccg ccgatgaagg tgttgcggcc caccacgcag ccctgcagca ccgtattctg
     4501 ggccaccata gtgttttcca tgaacgaggt gccgtagagc gcggcacgga acggaatata
     4561 acagccgttg ctgaccatgc tgagcacgat ttgggcgccc tgcataatgt tcacgttgtc
     4621 gccgatgacg ctggagacaa tcaggctgcc cgcgccgatg gtcacgttat tgccgatgat
     4681 cgtcggcccc tggatgatca cgccgggatc gatcttgcag ttgcgcccga tgcgcaccac
     4741 ggcggagcag gacaacgggt gcttgcgttc gaggaccacg cgcgtgagga tgttgagctt
     4801 gaccttgagc gagttcgact ctttgagcag ccggcgcgcc cagcccagca ccccgaacag
     4861 cgagttggtc agcatcactg cgacccaact ctcgatcggg atgaaggctt tgagg
//
LOCUS       DASUYC010000806         2065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1512450, whole genome shotgun sequence.
ACCESSION   DASUYC010000806 DASUYC010000000
VERSION     DASUYC010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512450"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VF276_13230"
     CDS             complement(<1..320)
                     /locus_tag="VF276_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX6607864.1"
                     /translation="MFRGLRLRLTALYLLAALALMLLIGAGSYWLLDRYFDSTTDLAL
                     QHKMVEAFRGLGATPPASLLAADAAWTANRPSVLPALSPTAAREKDHDEHKSEGSGDG
                     GEHSG"
     gene            complement(313..996)
                     /locus_tag="VF276_13235"
     CDS             complement(313..996)
                     /locus_tag="VF276_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6607865.1"
                     /translation="MRILIIEDDQRLTRLIARVLEDEHFSVDVAHDGETGLDLALRGA
                     YDVAIVDWMLPGRDGPSICRAVRARRLPLALLLLTARGQLEDRVAGLDSGADDYLVKP
                     FAFEELLARVRALGRRFQVLAGDGTELRAGDLVLDLRAHLARRGDQPLDLTATEWNLL
                     ECLVRHPGQALTRQQILDYVWSYEADVQATMVDVYISYLRRKLALPGRPDPIETVRGV
                     GYRLEPRRV"
     gene            1184..1789
                     /locus_tag="VF276_13240"
     CDS             1184..1789
                     /locus_tag="VF276_13240"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEX6607866.1"
                     /translation="MKQRIPLLLAAGLTAFMLVLGGALAAQLLAPVPAAPVVDAAAGS
                     DPALLAPPNDPTVQTLLDREAAYEQALQQANAQLQQAYQDQQQLADQVKVLQTQAQAA
                     PMDLPAPTSATAAPAATYALSPQQAALVALRAAPDATLTAAPDAVDFQGQVAYEVRLT
                     RGTVYVDANSGQVLYNGATAGRTGAGGEHDHNGYEEHESDD"
     gene            1782..>2065
                     /locus_tag="VF276_13245"
     CDS             1782..>2065
                     /locus_tag="VF276_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607867.1"
                     /translation="MTDTLIIDGSPRPKAPAGNKKAARAGRKSTFNMKLLISTAALAA
                     TLDGWALLAAHDQGLAAAPAPNTGAPAAVAAGGAAASGYLLDRPPIPTVVP"
BASE COUNT          380 a          733 c          662 g          290 t
ORIGIN      
        1 ccgctatgct cgccgccatc gccggatccc tccgacttat gctcgtcgtg gtccttctcg
       61 cgggcggcgg tgggcgagag cgcgggcaac acgctgggcc ggttggcggt ccaggcggcg
      121 tcggcggcca gcaggctagc gggcggcgtg gctcccaggc cgcggaaggc ttcgaccatc
      181 ttgtgctgca acgccaggtc ggtggtgctg tcgaagtacc gatcgagcag ccagtagctg
      241 ccggcgccga tgagcagcat cagcgccagg gcggccagca ggtagagcgc cgtcaggcgc
      301 aggcgcaagc ccctaaacac ggcgcggctc cagccgatag ccgacgccgc gcaccgtctc
      361 gatggggtcg ggccggcccg gcagggccag cttgcggcgc aggtaagaga tatagacatc
      421 gaccatcgtc gcttgcacat cggcctcata ggaccagacg tagtcgagaa tctgctggcg
      481 agtaagcgcc tggccgggat ggcgcacgag gcattccagc aaattccact cggtggcggt
      541 gagatcgagc ggctggtccc cgcggcgggc gagatgggcc cgcaggtcca gcaccaggtc
      601 gccggcgcgg agttcggtcc catcgcccgc gagcacctgg aagcgccggc ccaacgcgcg
      661 gactcgcgcc agcaattcct caaaggcgaa gggcttgacc agataatcgt ccgcgccgct
      721 gtccaggccg gccacgcgat cttcgagttg cccgcgcgcc gtcagcagca gcagggccag
      781 cggcaagcgc cgggcacgga ccgcccggca aatgctcggc ccatcgcgcc ccggcagcat
      841 ccaatccaca atggccacgt cataggcccc ccgcaacgcc aggtccaacc cggtctcgcc
      901 gtcgtgggcc acgtccacgc taaagtgttc gtcttccagc acccgcgcga tcaggcgcgt
      961 gaggcgctgg tcgtcttcaa tgatcaagat gcgcatgatt cggtcctcgc ctgcctggat
     1021 ccatcatagg cccgcgggat tggaaataga ttggaaaatt cggcacccgc ccccatggcc
     1081 ttttaacctt gctccgctat gcttaacaac gaaggcgcaa accttccgca aagccatcca
     1141 cacgaaccat ctaccaaacc gtcccatcaa aggagcctgc tgaatgaagc aacgcattcc
     1201 cttactgctc gctgccggat tgaccgcgtt tatgctggtg ttaggcggcg cgctggccgc
     1261 acagttgctg gcgcccgtcc ccgcggcccc cgtggtcgat gcagcggccg gcagcgatcc
     1321 ggccctgctc gccccaccca acgatccgac cgtgcaaacc ctgctcgacc gcgaagccgc
     1381 ctatgaacag gcactgcagc aggctaacgc gcaattgcag caagcctacc aggatcagca
     1441 acagttggcc gaccaggtga aggtgctgca aacgcaagcg caagccgcgc ccatggacct
     1501 gcccgcccct accagcgcga ccgccgcgcc ggcggccacc tacgcgctct cgccacagca
     1561 ggccgccctg gtggcgctcc gcgcggcacc cgatgcgact ctgaccgccg cgccggacgc
     1621 ggtcgatttc cagggccagg tggcctatga agtccggctg acgcgtggga cggtctatgt
     1681 cgacgcgaac agcgggcagg tactgtacaa cggcgcgacc gccggccgca ccggagccgg
     1741 cggcgaacac gaccataacg gctatgagga acatgagagc gatgactgat acgctaatca
     1801 ttgacggctc ccccagaccg aaagctccgg cagggaacaa gaaggcggcg cgggccgggc
     1861 gcaaaagcac gttcaacatg aagctgctga tctcaaccgc cgccctggcc gccacgctcg
     1921 acggatgggc gctgctggcc gcgcacgatc agggcctggc cgccgcgccg gcaccgaaca
     1981 ccggcgcacc tgctgccgtc gcggccggtg gagcggcggc aagcgggtat ctcctagacc
     2041 gcccgcccat cccgaccgtg gtgcc
//
LOCUS       DASUYC010000807         2051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1050378, whole genome shotgun sequence.
ACCESSION   DASUYC010000807 DASUYC010000000
VERSION     DASUYC010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050378"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="VF276_13250"
     CDS             <1..790
                     /locus_tag="VF276_13250"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX6607868.1"
                     /translation="LTLRDVTLERATERAKSDFLSIVSHELRTPLNSIMGFLDIILMG
                     KTGPLTDLQQDFLSTAKHESVALHRLINDILDYSQVQSGMLRLEMLPMDLSAALTRVV
                     NHAGPRMAEDKLTLVNDVAPQVLVMGDEVRLEQVFKNLLDNAAKFTDVDGEIRLTATV
                     DVNTVLVSVKDTGCGIPLAQVGHVFDRFFQAENRSNRRKRGLGLGLAICRNIIEAHGG
                     RIWIESEPGVGTTVFIELLLFRPDSENDLYKFDVKTGEATFTRR"
     gene            1084..>2051
                     /locus_tag="VF276_13255"
     CDS             1084..>2051
                     /locus_tag="VF276_13255"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607869.1"
                     /translation="MLIEDSKQTAVITRANGAGAAADAEFEALSQALAARVHEADQAE
                     RRRAELRAARWPRMLVRILIDVVLLNIAFMLAYWARYEAQVLRDVAPEFYHEMSDFVI
                     FQMLFLVIGPVVLYARGVYAQPRGSSLFDQVGRIAGAGMFTVCAVLLTSLIFESGVPS
                     RLFFVYLWLAIIVTFGAERYLIKQVRRVLWSRGINEHRVIIVGATPAGQRVMKHILDR
                     PAMGYHLIGYLDDRAQERRWAVPFPGKPRPARLGSVKDLEQIIADQHVDEVIIALGAT
                     RRTNLSGLLARCSALRVNYRLVPDLFEMRFDHTETESLDGIPLIGL"
BASE COUNT          347 a          686 c          660 g          358 t
ORIGIN      
        1 gctcaccctg cgcgacgtga ccctggagcg ggccaccgag cgcgccaagt ccgacttcct
       61 gtccatcgtc tcgcacgagc tccgcacgcc gctcaactcg atcatgggct ttctcgacat
      121 catcctgatg ggcaagaccg gcccgctgac cgatctgcag caggacttcc tgtccacggc
      181 caaacacgag tcggtggccc tgcaccgcct gatcaacgac atcctcgact actcgcaggt
      241 gcagagcggc atgttgcggc tcgaaatgct gcccatggac ctgtcggcgg cgctgacccg
      301 cgtggtcaac cacgccgggc cgcgcatggc cgaggataag ctcaccctgg tcaacgacgt
      361 ggccccgcag gtgctggtca tgggcgacga agtgcgcctg gagcaggtgt tcaagaacct
      421 gctcgacaac gccgccaagt tcaccgacgt ggacggcgaa atccgcctga ccgcgacggt
      481 cgatgtcaac acggtgctgg tcagcgtgaa ggacaccggc tgcggtatcc cgctggccca
      541 ggtgggccac gtgttcgacc gcttcttcca ggccgagaac cggtccaacc gccgcaagcg
      601 cggcctgggc ctgggcctcg ccatctgccg taatatcatc gaagcccacg gtgggcgcat
      661 ctggatcgaa agcgagccgg gcgtcggcac cacggtgttc atcgagttgc tgctcttccg
      721 cccggacagc gaaaacgacc tgtacaagtt cgatgtgaag accggcgaag cgaccttcac
      781 ccggcgctag cccggccctg cgcgaatacc gttccaccgg ggccgtcccg ccgcatgcgg
      841 agacggtccc ggcgtttttg gcccgcgtcg cgttgccgcg ataaaacttg cggctttgta
      901 agcaccccgg cgcagggcct acagccagtt gtaaatcgat agtggtacat tgagattgca
      961 atatcggaac acgaccggag cgcgagccac caggcgccgg ggttccggta agggggcgca
     1021 gctacccgga attacccggc cccgtatgat ctcgataggg cgagacacca cagtgaggtc
     1081 gtcatgttga ttgaggacag caagcagaca gcggtcatca cgcgggccaa tggcgcgggt
     1141 gcggcggcag acgcggagtt cgaggcgcta tcgcaggcgc tggcggcgcg ggtgcatgag
     1201 gctgaccaag ccgagcggcg ccgcgccgag ctccgggcgg cgcgctggcc gcgcatgctg
     1261 gtgcgtatct tgatcgacgt ggtgctgctc aatatcgcct ttatgctcgc ttactgggcg
     1321 cgctatgaag cccaggtgct gcgcgatgtg gcgcccgaat tctatcacga gatgtctgat
     1381 ttcgtgatct tccagatgct gtttctggtc atcgggccgg tggtgcttta tgcccgcggg
     1441 gtctacgccc agccgcgcgg cagttccctg ttcgaccagg tgggccgcat cgccggcgcg
     1501 ggcatgttca ccgtctgcgc ggtactgctg accagcctga tctttgaatc cggcgtgccc
     1561 tcgcggctct tcttcgtcta cctctggctg gcgatcatcg tcaccttcgg cgccgagcgc
     1621 tacctgatca agcaggtgcg ccgagtgctg tggagccgcg gcatcaacga gcaccgcgtg
     1681 atcatcgtcg gcgccacccc cgcgggccag cgcgtcatga agcacatcct cgaccgcccg
     1741 gccatgggct accacctgat cggctacctg gacgaccggg cgcaggagcg gcgctgggcc
     1801 gtgccgttcc ccggcaaacc ccggcccgcg cgcctgggca gtgtgaagga cctggagcag
     1861 atcatcgccg atcagcacgt cgatgaagtg attatcgcgc tgggcgccac ccggcgcacc
     1921 aacctgtcgg gcctgctggc gcggtgcagc gccctgcgcg tcaattaccg gctggtcccc
     1981 gacctgttcg agatgcgctt cgatcacacc gagacggagt cgctcgacgg catcccgctc
     2041 atcgggctga a
//
LOCUS       DASUYC010000808         8053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_94350,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000808 DASUYC010000000
VERSION     DASUYC010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 8053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8053
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94350"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VF276_13260"
     CDS             complement(<1..252)
                     /locus_tag="VF276_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX6607870.1"
                     /translation="MPIYEYRCPDCKRRVSVFFRTMSAAESGASTAACPNCGGQHLQR
                     LVSKVAYIRAGNLGDNPNLGGATTVDDLDDENAYGSYDDS"
     gene            575..1756
                     /locus_tag="VF276_13265"
     CDS             575..1756
                     /locus_tag="VF276_13265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6607871.1"
                     /translation="MAIQTASPAPVRATAPSVPPYTLIVLSHLGWDWVWQRPQHLLSR
                     LARRHPVFFVMEPLLDENPAAPYLKIEPRGPGVTVAAPVFSLEQADAAGGFYPLIGQL
                     VDAWIAQAQVRNPVFWLYTPMAQPVVANRPGAVVVFDAMDELAAFRFAPPVLKAREAA
                     LMQRADLVFTGGQSLYEARRGRNPHVYCFPSGVERAHFAAALDRDTPEAPALAGLPHP
                     RIGFYGVIDERMDLDLLAQVAALRPQYQWIMVGPTAKIEAADLPQAPNILYPGKQEYA
                     ALPGFLKGFDVCMMPFALNESTRFISPTKTLEYMAAHKPIVSTPITDVAGPYGHIVRL
                     AGTAEEFVAAVDAALAEDRTVRAARVAAENNVLARQEWDRIAAEMERLIDACYVARVA
                     R"
     gene            complement(1793..3472)
                     /locus_tag="VF276_13270"
     CDS             complement(1793..3472)
                     /locus_tag="VF276_13270"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6607872.1"
                     /translation="MPVGGPATSTANAAPSSPWSPLAQPVFRALWIASVASNIGTWMQ
                     SVGAAWLMTSLTSSAVLVALLQTATSLPVFLVGLPAGALADVIDRRRLLLVTQAWMLA
                     TACLLGALTLAGAMSAWGLLALTFLLGLGAALNAPAWQAIVPELVGRRDLAVAVALNS
                     AGFNLARAVGPALGGLIVAALGPGAVFLLNAASFLGVLLVVYRWRRTPETTTAPSERV
                     FGAIQAGVRYARYAPRLRAVLVHTGTFIFAASGLWALLPVVARSELGLDATGYGVLLG
                     SLGLGAVGGAALLPRFRRALPVDRLVVAATVVFAIATLALGYIPNLLLLDATLIAGGI
                     AWITLTSSLNVSAQSTAPAWVRARVLGVYLLVFQGGLAAGSFVWGALATQFGNPPALG
                     VAALALLAGAAAARRWRLQPVEEIDLTPALYFAEPRLILEPDPEDGPVLVTAEYQVPN
                     ANARAFVQAMEAMGRHRRRDGAMRWGLFRDPAAPEHYIETFVVASWAEHMRQHERATA
                     SDEMTRAHALTFVAPGTAPVVSHYIAAQALDPDLAAAPAAPVPATEGAANP"
     gene            complement(3523..3873)
                     /locus_tag="VF276_13275"
     CDS             complement(3523..3873)
                     /locus_tag="VF276_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015393766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607873.1"
                     /translation="MQPYSIWIEAEQWAPGHWTPADENSDVIITFEDGSRWVATFFSY
                     TNINTLVEKNRRTGEYMSGKYFWAGDMILVDEVSRARVEEVVRYLLETREFKQIFTGL
                     PPAIDEGDPTTPSG"
     gene            4073..4462
                     /locus_tag="VF276_13280"
     CDS             4073..4462
                     /locus_tag="VF276_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX6607874.1"
                     /translation="MIPSKYTDLLKSNALAHVATVGPDGAPQSNPVWFDWDGEHVLFS
                     QTKTRQKYKNVQRDPHVALSIVDPSNPFRYLEIRGTVSKIEEDPDNRFINAMAKKYLG
                     QDKYPWNRPGDERIVVYVTPEHTTHMG"
     gene            complement(4776..7268)
                     /locus_tag="VF276_13285"
     CDS             complement(4776..7268)
                     /locus_tag="VF276_13285"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6607875.1"
                     /translation="MNETLSSGAWMKRRRRELGLTQEALAERAGCSWETVRKIEAGTL
                     RPSRQLAELLAIQLAVPAAERADFVRWLRATPLPAGEEQHRPAAPLAHTRNLPLVPTS
                     LIGRATEVARLRRLLQQFEVRLVTLVGPPGIGKTRLALAVAASLRDEFADGVWFVELA
                     PLTDAGLVLPAIAGTQGVTEDDKQMLRDKLLEYLHEKQVLLVLDNFEHLPAATALVDE
                     LMRATPGLKVLATSRIPLRLYGEREYPVPPLRLPDARADQTLDVLRQYDAVQLFTERA
                     REVQPDFALTTENAGAVGQICLRLDGLPLAIELAAGRVRLFPPPALLARLAARLPLLT
                     GGARNLPARHQTLRGAIAWSYDLLAAAERQLFRRLAVFQGGCTLEAIAAVCNAQGDLP
                     LEVLDGVESLVEKNLLQQREGRATEPRFFMLDTIQEYAWEQLAASGEEAALRLQQARY
                     LVQIAEVVDPYLDVEQVPASLDRLEEELPNIRAALEWLLAEAEDEPGEKLALGLRLVG
                     ASGALWGSRGYAREGRRWVIAALVRNAQWMPGTAPRAEPAGFQRGLFSALVCAGHLFW
                     GYDDAAARGFFEQSLSISRAMNDAHCICLALIGLGNVSGDQGDAALARACFEECLALG
                     RAAGDQWALNQALNNLGCTALENEDYSTARSLFEESLRGRCAMGHKGQIAQTLDNLGN
                     LAYLQGDYEAARTYLQESLGLVMQSAAPLGIVTALAVLAGVEAQRAASVDTPPPAASH
                     KRPAGEQNSAHVPLLAGQRATRLLGASMEWLETHGEQLERVERRISEQAKTRLQALLG
                     AAEFERTWQEGRAMTLEEAAAYALEEWEEGKT"
     gene            complement(7400..>8053)
                     /locus_tag="VF276_13290"
     CDS             complement(7400..>8053)
                     /locus_tag="VF276_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607876.1"
                     /translation="GYGLRLWKRVELPEVYMAGFLAILFSWPWTGDRFLLPVLPFILH
                     YIAEALVALAGAVGRLLRRPRVAWAAPALLGLLLVPSLAQDSIAIGANLRYLGGDIAA
                     GGLTVEDRDFLAASVWLRDHTPPGGVVLARKSSIAEIYSGHPAELIPLIPPDEYLAWL
                     RARQVTYVIEDAWPWSRHTIVYLRPAMAAYPAQFRLLHTIPAATPGGTPTRIWQFLP"
BASE COUNT         1366 a         2761 c         2619 g         1307 t
ORIGIN      
        1 ggagtcgtcg tagctgccgt aggcgttctc gtcgtccaga tcgtcgacgg tggtggcccc
       61 gcccaagttg gggttgtcgc ccaggttgcc ggcgcggata taggccacct tcgagaccag
      121 ccgctggaga tgctgcccgc cgcaattggg gcaggcggcc gtgctcgccc cgctctcggc
      181 ggcgctcatg gtacggaaaa agacgctgac ccggcgtttg caatcggggc agcgatattc
      241 gtaaataggc atggtaactt ccttcgtctg atatgcgcgg tagatggatc gcgcaagttt
      301 cccctattct accatagccg ggcgtgcaga ccaaagaggc acccggaggc ttgcaccgcc
      361 gggcgcccgg ctgtcgcaaa acccgacatg cgttccagat tggcataatt cctgctttac
      421 caaaggggca caaccgtagc catagatgag cggctacatg gccctcgcga tccggattct
      481 ccgcgcccgg cgctcagggc ctgtcagagc tgcaccggca ggcagccctg gtcatttcgc
      541 ttgtaggggc cggccatcac ggaggagttg cacattggcg atacaaacag catccccggc
      601 gccggtacgc gcgacggcgc cttccgtacc cccgtatacc ttgattgtcc tctcgcatct
      661 cggctgggat tgggtctggc agcgcccgca gcatttgctg tcgcgcctgg cccgccgcca
      721 cccggtcttc ttcgtcatgg aaccgctgct ggacgagaac ccggcggcac cctacctgaa
      781 aatcgagccg cgcgggccgg gggtgacggt ggccgcgccg gtgttctcgc tggaacaggc
      841 cgatgcggcc ggcggttttt acccgctcat cgggcaactg gtggacgcct ggatcgccca
      901 ggcgcaggtg cgaaacccgg tgttctggct ctatacgccg atggcccagc cggtggtcgc
      961 caaccggccc ggcgctgtcg tggtgttcga cgccatggat gaactggccg ccttccggtt
     1021 cgcgccgccg gtgttgaagg cgcgcgaggc cgccctgatg cagcgggccg acctggtgtt
     1081 tacggggggc cagagcctgt acgaggcgcg gcgaggccgc aacccccatg tctattgctt
     1141 ccccagcggc gtcgagcgcg cccacttcgc ggcggcgctg gatcgcgaca cgcccgaagc
     1201 acccgccctg gccgggctgc cgcacccgcg tatcggcttc tacggtgtca tcgacgaacg
     1261 gatggacctc gacctcctgg cgcaggtggc ggcgctgcgc ccgcagtacc agtggatcat
     1321 ggtcggcccg acggcgaaga tcgaagccgc cgatctgccc caggcgccga atatcctcta
     1381 tccgggcaag caggagtacg cggcgttgcc tggtttcctg aagggcttcg acgtgtgcat
     1441 gatgccgttc gcgctcaacg aaagcacgcg cttcatcagc cccacgaaga ccctggaata
     1501 tatggccgca cacaagccga ttgtttccac gccgatcacc gacgtggccg ggccgtatgg
     1561 gcatattgtc cgcctggccg gcacggcgga ggagttcgtc gccgcggtgg acgccgccct
     1621 ggccgaggac cgcaccgtgc gcgctgcccg tgtcgccgcc gagaacaacg tgctggcccg
     1681 ccaggagtgg gatcgcatcg ccgcggagat ggagcggttg atcgacgcct gctacgtggc
     1741 tcgcgtggcc cgttaacctg cacctcgcta cggggtgcgc caaatcccgg tgctagggat
     1801 ttgccgcccc ttcggtggcc ggcacgggag cagccggcgc ggcggccagg tccgggtcca
     1861 gggcctgcgc ggcaatgtaa tgcgaaacca ccggtgcggt gccgggcgcc acgaaggtca
     1921 aggcatgcgc ccgcgtcatc tcgtcggagg cggtggcccg ctcgtgctgg cgcatgtgct
     1981 cggcccacga ggcgacgaca aaggtttcga tgtagtgttc gggcgcggcg ggatcgcgga
     2041 acaggcccca gcgcatggcc ccgtcacgcc gccggtggcg gcccattgcc tccatcgcct
     2101 gcacgaaggc gcgcgcgttg gcgttgggca cctggtactc cgctgtcact agcactggcc
     2161 cgtcctccgg atcgggttca aggatcagcc ggggttcggc aaaatagagc gccggggtga
     2221 ggtcgatctc ctccaccggc tgcaagcgcc agcgccgcgc cgcagcggcc ccggccagca
     2281 gcgccagcgc cgcgaccccc agggccggcg ggttgccgaa ctgggtcgcc agcgcccccc
     2341 acacgaagct gcccgccgcc agcccgccct ggaacaccag cagatagacc cccagcacgc
     2401 gggcgcgcac ccaggccggc gcggtgctct gggccgacac gttcaggctc gatgtcagcg
     2461 tgatccaggc gatgcccccg gcgatcaagg tcgcgtcgag tagcaacagg ttggggatat
     2521 agcccagggc cagggtggcg atggcaaaga cgacggtggc cgcgacgacc agccggtcca
     2581 ccggcagcgc gcgccggaag cgcggcagca gcgccgcgcc gcccaccgcg cccaggccta
     2641 ggctgcccag cagcacgccg tagccggtcg catccaggcc gagttcgctg cgcgccacca
     2701 cgggcagcag cgcccagagc ccgctggccg cgaagatgaa cgtgccggtg tgcaccagga
     2761 ccgcgcgcag gcgcggggcg tagcgagcgt agcgcacgcc cgcctggatc gcgccgaaca
     2821 cccgctcgct gggcgcggtg gtcgtctccg gcgtgcgccg ccagcggtag accacgagca
     2881 ggacgccgag gaacgacgcg gcgttgagca ggaacacggc gcccggccct agcgcggcca
     2941 cgatcagccc gcccagggcc gggcccacgg cacgcgccag gttgaacccg gcgctgttga
     3001 gcgccacggc caccgccagg tcgcgccgcc ccaccagttc gggcacgatg gcctgccagg
     3061 cgggcgcgtt gagcgccgcg cccaggccca gcaggaacgt gagcgccagc agcccccagg
     3121 cggacatggc cccggccagc gtcagcgcgc ccagcaggca ggccgtcgcc agcatccacg
     3181 cctgcgtcac cagcagcaac cgccgccggt cgatcacgtc ggccagcgcg cccgccggca
     3241 ggccgaccag gaagaccggc aggctggtcg ccgtttgcag cagcgccacc agcaccgccg
     3301 acgacgtgag cgaggtcatc agccaggcgg cgcccacgct ctgcatccag gtgccgatat
     3361 tcgacgccac cgaggcgatc cagagcgcgc ggaacaccgg ctgggccagc ggactccacg
     3421 gtgaactggg cgcggcattg gcagtagacg tagccggacc tccaacaggc agagtagcgt
     3481 gtgcgggtag tgtcgctcat tcagcaaatc atgggccgca atctacccgc tcggcgtggt
     3541 gggatcgcct tcgtctatgg ccggcggcag cccggtgaag atctgcttga actcgcgtgt
     3601 ttccagcaga tagcggacga cctcttcgac acgggcgcgg ctgacctcgt ccaccaggat
     3661 catgtcgccg gcccagaagt attttccgct catgtactcg ccggtgcggc ggttcttctc
     3721 aaccagcgtg ttgatattgg tgtaggagaa gaaggtcgcc acccagcggc tgccgtcctc
     3781 gaatgtgatg attacatccg aattctcatc cgcgggcgtc caatggcccg gcgcccattg
     3841 ctcggcttca atccagattg aatatggttg catcgctaat tcctcatctc gcagtccgcg
     3901 ttggctcact ttatgcggta attatcgcat agtgccgtga ttgcagctaa gggacgtcct
     3961 gaaggacact accggggaca ctgccctgtt ataatcggca caaataagcg gcaattggca
     4021 accgcccggt atcggccggg cacgacgact gagtgaaact ggagaaacct gaatgatccc
     4081 gtcgaagtac accgatctgt tgaaatcgaa cgcgctggcc catgtagcga ctgtgggccc
     4141 ggatggcgcg ccgcagtcga accccgtctg gtttgactgg gacggcgagc acgtgctgtt
     4201 cagccagacc aagacccgcc agaagtacaa gaacgtgcag cgcgacccgc atgtggccct
     4261 gtccatcgtc gatccgagca atcccttccg ctacctcgaa atccgcggca cggtcagcaa
     4321 gatcgaggag gacccggata atcgcttcat caacgcgatg gcgaaaaagt acctgggcca
     4381 ggataaatac ccatggaacc gtccgggcga cgagcggatt gtggtctacg tcacgcccga
     4441 gcacactacg cacatgggct gagccggcac gacggcagtt tcaataaaaa gggcggcaca
     4501 agcaaagtgc cgcccttttt gctgtcgatg tcccttcgtc tctacttcaa ggccgcgccc
     4561 gctctcaccc ttgtcactct gagcgcagcg aagagtctcg tccccgtcga agatgccggt
     4621 accacccgcg gtagcagcgc cgggtgcgtc acacgacagg ctcaaccgtt agagtcagca
     4681 agcctggcgt aaagattcgg tggtaacttt ccgccctctt tctttgtctt gtagcccagc
     4741 cggtcgttgg tcagtgccgg ccacactaca gaggatcagg tcttgccttc ttcccactcc
     4801 tccagagcgt aagcggcagc ttcttcaagc gtcatggccc gcccttcctg ccaggttcgc
     4861 tcgaattccg ctgctcctag caatgcctgc agtctggtct tcgcctgctc ggatatgcga
     4921 cgctccaccc gctccaactg ttccccatgt gtctccagcc actccattga agctcccagt
     4981 agccgggtcg cccgctggcc tgccaggagc ggcacgtgcg cgctgttctg ctcgccggcc
     5041 ggtcgcttgt ggctcgccgc gggtggcgga gtgtctacac tggcggcccg ctgcgcctcc
     5101 acaccggcca ggacggccaa cgccgtgact atgcccaatg gtgctgcgga ctgcatcacc
     5161 agacccaggc tctcctggag atacgtgcgc gcggcttcgt aatctccttg aagataggcc
     5221 aggttgccca gattgtccaa cgtctgggcg atctggccct tgtggcccat ggcgcatcta
     5281 ccccggaggc tctcttcgaa aagggatcgt gcggtggagt agtcctcgtt ctccagcgcg
     5341 gtgcatccga gattattcag cgcttgatta agggcccatt ggtcgccagc agcccgaccg
     5401 agagccaggc attcctcgaa gcacgccctg gctagcgcgg catccccctg gtcgccactc
     5461 acattcccca atccgattaa ggccaggcag atgcagtgcg cgtcgttcat cgcccggctg
     5521 atgctcaagc tctgctcaaa gaagccacgc gcggcggcat cgtcgtagcc ccagaacaag
     5581 tgaccggcgc agacgagcgc gctgaatagc ccccgttgga accccgccgg ctcagcgcgt
     5641 ggggcagtcc ccggcatcca ctgtgcgttg cggaccaggg ccgctatgac ccagcgccgc
     5701 ccctcgcggg cgtatccacg cgacccccag agcgcacccg aagctccaac cagtcgcaac
     5761 ccgagcgcca gcttctctcc cggctcgtct tccgcttcgg ctagcagcca ttccagcgcg
     5821 gcccggatgt tcggcaactc ctcttccagc cgatccagcg acgccggtac ctgctctacg
     5881 tccaggtacg gatccaccac ttccgcaatt tgtaccaggt accgggcctg ctggagtcgc
     5941 agcgccgcct cttcgccgct tgccgctagt tgctcccagg cgtactcctg gatcgtatcc
     6001 aacataaaga agcgcggctc ggtagctcgc ccttcccgct gctgcaacag gttcttctcg
     6061 accagggact ctactccatc cagcacctcc aggggcagat cgccctgtgc attgcagacc
     6121 gccgcgattg cctcaagggt gcatcctccc tggaacacgg ccaaccgccg gaatagctgc
     6181 cgttctgctg ccgccagcag atcataactc cacgcgatgg cgccgcgcag cgtctggtgg
     6241 cgggccggca ggttgcgggc accgccggtc agcaagggca agcgcgctgc caaccgggcg
     6301 agcaaggccg gcggcgggaa cagccggacg cggcctgcag ccagttcaat agccaacggc
     6361 aggccgtcga gccggaggca gatttgcccc acggcgccgg cgttttccgt ggtcagggca
     6421 aaatcgggct gcacctcccg tgcccgctca gtaaagagtt gtaccgcgtc gtattgtctg
     6481 agtacatcca gggtttggtc ggcccgcgca tccgggagtc gcaggggcgg caccggatac
     6541 tcgcgttcgc cgtacaatcg cagggggatg cggctggtcg ccagcacttt caggccgggg
     6601 gtcgcccgca tcaactcgtc gaccagcgcg gtcgccgcgg gcaggtgctc gaaattgtcc
     6661 agcaccagca acacctgctt ttcgtgcaag tactccagaa gcttgtcccg cagcatttgc
     6721 ttgtcgtctt cggtcacgcc ctgcgtgccg gcgatggccg gtagcaccaa tccggcatcc
     6781 gtgagcggcg cgagttcgac aaaccagact ccatcggcga attcgtcgcg caggctggcc
     6841 gccaccgcca gggcgaggcg ggtcttgccg atgcccggcg ggcccaccag ggtaaccagc
     6901 cgcacctcga actgctgcag tagccggcgc aaccgggcga cttcagtcgc ccggccaatc
     6961 agcgaagtcg gcacgagggg tagattgcgg gtgtgcgcaa gcggggcagc cgggcggtgt
     7021 tgctcctcgc cggcgggcag gggcgtcgcg cgcagccagc gcacgaaatc ggcgcgctcc
     7081 gcggccggca cggccaactg gatggccagc aactcggcca attggcggga ggggcgcagg
     7141 gtgccggctt cgattttgcg taccgtctcc cacgagcagc cagcgcgctc ggcgagcgcc
     7201 tcctgcgtca gacccaactc gcgccgcctt cgcttcatcc aggcccctga agacaaggtc
     7261 tcgttcatgg ctcgttgccc gacccactga tgtgcgactt tatccgcgtt gccctcgccg
     7321 caattatact acctttgctt gctgaatagg acccttcgcg aagaaagacg tgccgtgccc
     7381 caaaaggtaa cgcggcgggt cagggcagaa actgccagat acgggtcggt gtgccgccgg
     7441 gcgtggccgc cgggatggtg tgcagcaggc ggaactgggc cgggtaggcg gccatggccg
     7501 ggcgcaggta gacgatggtg tgccgcgacc agggccaggc gtcttcgatg acgtaggtga
     7561 cttggcgggc gcgcagccag gccaggtact cgtcgggcgg gatgagcggg atcagttcgg
     7621 cggggtggcc gctgtagatc tcggcgatgg agcttttgcg cgccagcacc acgccgccgg
     7681 gcggcgtatg gtcgcgcagc cagacggagg cggccaggaa gtcgcggtcc tcgacggtca
     7741 gcccgcccgc cgcgatgtcg ccgcccaggt agcgcaggtt cgcgccgatg gcgatgctgt
     7801 cctgggccag gctgggcacc agcagcaggc ccagcagcgc cggggccgcc caggccaccc
     7861 gcgggcggcg cagcagccgc cccaccgcgc ctgccagggc caccagtgcc tcggcgatgt
     7921 agtggaggat gaagggcagc accggcagca ggaagcggtc gccggtccag ggccagctaa
     7981 acaggatcgc caggaacccg gccatgtaga cctcgggtag ctcgacccgc ttccacagcc
     8041 gcaagccgta gcc
//
LOCUS       DASUYC010000809         3040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1522686, whole genome shotgun sequence.
ACCESSION   DASUYC010000809 DASUYC010000000
VERSION     DASUYC010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1522686"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..2475)
                     /locus_tag="VF276_13295"
     CDS             complement(133..2475)
                     /locus_tag="VF276_13295"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HEX6607877.1"
                     /translation="MEKHTHPTMGATADRHPGSAEQANSHVPLPFPVRYRRALDNPQL
                     QRTLRNFQRNWRESRAAAFDAYAANPLHALVDTDSPRRIHIPVPNPDTPGGAEFAALR
                     DRLAAIKDQVIDDLPAYVDQFQREAEARGVHVYRAADAAAANRYVLDLCRRKGIGHVV
                     KSKTMVSEEIDLNAALEAAGIQAVETDLGEWIQQLSHERPSHMVMPAIHRTRQEVGDL
                     FTEATGRPVSRDDIGEQVGVARRELRRDFLEAGLGVSGANALIASSGTVMFIENEGNA
                     RLVTSLPRVHVVLAGIEKLVPDHAAAMLQLRLLARSATAQQITSYSTFVSGPPEPGKE
                     MHIVLVDNGRTTMRETPLIRDALRCIRCAACANVCPPYAEVGGHVFGYIYSGAIGLVN
                     TPFHHGLEAGAGPQSLCVSCNACQTVCPVGIPLPRQILAVRARVVEAKGMALPVRLGL
                     EVWSRPRLADLAVRFSAVLGKPFFKGRFVDPARIGAGVIPPVRNLAGWRTPPAPARVP
                     ARDRLRRRPPVAVIPGSKAAGLRITYFTQCLTDRLYPDMAAATVRVLEACGAEVTVPD
                     DQHCCGLPAIDAGDPELGRRMARQTIEVLERAGGDLIVTGAASCAIAVVHDYESLFAG
                     DQEWLERAAAVASRTMDFVSFLDEVAQLPAGALRRARPVGPVTYHSFCQSTNVLGLGR
                     APRRIIRDVLGLELIELPEGTVCCGFGGSTSATRPEVAARILERKLANVRATGARILV
                     TDNPGCILHLRGGVRAQHLPVHVLHIAELVAAHLPAQPGG"
     gene            complement(2479..>3040)
                     /locus_tag="VF276_13300"
     CDS             complement(2479..>3040)
                     /locus_tag="VF276_13300"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HEX6607878.1"
                     /translation="LFTQYATAAGAQVQTAGSPEAAAQAIAALRQGTLTCTAAVRDAY
                     PQIYAALAGPDGPVRVADDVAAEAADSTALAASLAGGTGLVVATAGVAETGSLLLTDN
                     ALAPRLLGMLADYCIALLDADTILPDLDVAGEWLARLERAGHRYQSLVTGPSRTADIE
                     RVLTIGVQGPKTLSIIVVHAPAGQEG"
BASE COUNT          434 a         1096 c         1095 g          415 t
ORIGIN      
        1 tgaacaggcg cgtgagacgg aagcgccgcg agacccggcg cggcagcacg atcgtctcaa
       61 aggcgtcgag cagcacgact ccgagcagga tcagccccac cacgtcggcc aggatatgca
      121 tgcaggcgac tcctagcccc ccggttgcgc cggcaggtgg gccgccacca gttcggcgat
      181 atgcaacacg tgaaccggca ggtgctgggc gcgcacgccg ccgcgcaggt gcaggatgca
      241 gccggggtta tccgtgacca ggatgcgcgc ccccgtcgcg cgcacgttgg ccagcttgcg
      301 ctccaggata cgcgccgcca cctccgggcg cgtggccgag gtcgagccgc caaagccgca
      361 gcagaccgtg ccctccggca actcgatcag ctccagcccc agcacgtcgc gaatgatccg
      421 gcgcggtgcg cgccccaggc ccagcacatt ggtgctctgg cagaagctat ggtacgtcac
      481 cggccccacc gggcgcgccc ggcgcagcgc gcccgccggg agttgcgcca cctcgtccag
      541 gaagctcaca aagtccatcg tccgcgacgc caccgccgcc gcgcgctcca gccattcctg
      601 gtcgcccgcg aacagcgact cgtagtcgtg gaccacggcg atggcgcaac tggcggcccc
      661 ggtcacgatc aggtcgccgc ccgcgcgctc cagcacctcg atagtctggc gggccatgcg
      721 gcggcccagt tccgggtcgc ccgcgtcgat ggcgggcagg ccgcagcagt gctggtcgtc
      781 cggcacggtg acctcggcgc cgcaggcttc gagcacgcgc acggtcgccg ccgccatgtc
      841 gggatagagc cggtcggtga ggcactgcgt gaaataggtg atgcgcagcc ccgcggcctt
      901 gctgcccggt atcacggcga ccggcgggcg gcggcgcagc cggtcgcggg cgggcacgcg
      961 ggcgggcgcg ggcggcgtgc gccagcccgc caggttgcgc accggcggga tcaccccggc
     1021 cccgatccgc gccgggtcca cgaaacggcc cttgaagaac ggcttgccca gcaccgccga
     1081 gaaacgcacc gccaggtcgg ccagccgggg ccggctccag acttccagcc ccaggcgcac
     1141 cggcagcgcc atccccttcg cctcgaccac ccgcgcccgc acggccagga tctggcgggg
     1201 cagcgggatg ccgaccgggc agaccgtctg gcaggcgttg cacgacacgc agaggctctg
     1261 cggcccggcg ccggcctcca ggccgtggtg gaacggcgtg ttgaccaggc cgatggcgcc
     1321 ggagtagatg tagccgaaga cgtggccgcc gacctcggca tagggcgggc agacgttggc
     1381 gcaggccgcg caacggatgc agcgcagggc atcgcggatc agcggcgtct cgcgcatggt
     1441 cgtgcgcccg ttgtccacca gcacaatgtg catctctttg cccggctccg gcggccccga
     1501 cacgaacgtc gagtacgacg tgatctgctg cgccgtggcc gagcgcgcca gcaggcgcaa
     1561 ctgcaacatg gccgccgcgt gatcgggcac cagtttctcg atcccggcca gcacgacgtg
     1621 aacacgcggc agcgacgtga ccaggcgcgc gttgccctcg ttctcgataa acatcacggt
     1681 gccgctgctc gcgatcagcg cgttggctcc gctgacaccc agcccggcct ccaggaagtc
     1741 gcgccgcagt tcgcggcggg ccacgcccac ctgctcgccg atgtcgtcgc ggctcaccgg
     1801 gcggcccgtg gcctcggtga acaggtcgcc cacctcctgc cgggtgcggt ggatggccgg
     1861 catgaccatg tgcgagggcc gttcgtgcga aagctgctgg atccactcgc ccaggtcggt
     1921 ttccacggcc tggatacccg cggcttccag cgccgcgttc aggtcgatct cctccgacac
     1981 catcgtcttg ctcttcacca catggccgat gcccttccgg cggcacaggt cgagcacata
     2041 gcggttcgcg gcggcggcat cggcggcgcg gtagacgtgg acgccgcggg cttcggcttc
     2101 ccgctggaac tggtccacgt aggccggcag atcgtcgatc acctggtcct tgatcgccgc
     2161 caggcggtcg cgcagggcgg cgaactcggc gccgccgggg gtgtcggggt tggggacggg
     2221 gatgtggatc cggcgcggcg agtccgtgtc cacgagggcg tgcagcggat tggcggcgta
     2281 ggcgtcgaag gcggcggcgc gggactcgcg ccagttgcgc tggaagttcc gcaaggtccg
     2341 ctgcaattgc gggttgtcca gcgcgcgccg gtagcggacc gggaagggca gcggcacgtg
     2401 gctgttcgct tgctcggcgc tgccggggtg gcggtccgcc gtggcgccca tggtcggatg
     2461 cgtgtgcttc tccatgcgtt acccttcctg ccccgccggg gcgtgcacga cgatgatcga
     2521 caatgtcttc ggcccctgca cgccgatggt cagcacccgc tcgatgtcgg cggtgcggct
     2581 cggcccggtg acgagcgact ggtagcggtg gcctgcgcgt tccaggcggg ccagccactc
     2641 ccccgccacg tcgagatcgg gcaaaatggt atcggcatcg agcagagcga tacagtagtc
     2701 ggccagcatg cccagcaggc gcggggccag cgcgttgtcg gtcagcagca gcgaaccggt
     2761 ctcggccacg cccgccgtgg cgaccacgag gccggtgccg ccggccaggg acgcggccag
     2821 cgccgtggag tccgccgctt ccgccgccac atcgtccgcc acccgcaccg gcccgtcagg
     2881 tccggccagc gcggcgtaga tctgcgggta ggcatcgcgc acggcggcgg tgcaggtcag
     2941 cgtgccctgg cgcagcgccg caatggcctg ggccgcggcc tccggcgagc cggcggtttg
     3001 cacctgcgcc ccggcggcgg tcgcgtattg ggtaaagagg
//
LOCUS       DASUYC010000810         3057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1743744, whole genome shotgun sequence.
ACCESSION   DASUYC010000810 DASUYC010000000
VERSION     DASUYC010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1743744"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="VF276_13305"
     CDS             complement(<1..872)
                     /locus_tag="VF276_13305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607879.1"
                     /translation="MTDHVKTSAAPGEPATRATPLPAGVPPGARPSPGPPAPDAAGAT
                     RGDRDSPAALPAVPAGADLAPRVVPMRWVALLCVLTFLVYLAFVPQIIRYSSPPTGDQ
                     PFYLMDTLSLVQDGDLELSNNYANHDEDKFYSLAPHPPDFVGMDAPYPLPRQLAMSSA
                     RPDQELYSFHPPGLGVLLVPAWIIGSWFALWWPTTVVFMCLVGALLATNIFLLAYETT
                     GKRWVALAVWAPLAFSGPLMSYSYMIFSELPTGLATTYAFRRLALGWRANGAGRLLLV
                     GAAIGYIPWLAVRCL"
     gene            1107..2921
                     /locus_tag="VF276_13310"
     CDS             1107..2921
                     /locus_tag="VF276_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX6607880.1"
                     /translation="MAYLPIENYGVIGDCHTVALVGLNGSIDWLCFPVFDSPSVFGAI
                     LDDEKGGHFQITPLEDDLMARQMYLPETNVLVTRFFSAAGLAEITDFMPIEDDKHATW
                     NHRVIRRVRVVHGSMRFKMECRPAFNYARTKHTVIADHDGVVFLSKDLSLGLASDTPI
                     KIDGPAVSSEFSLSVGQTTVFVLHRVDDDEPCGEYLKEDEVEQIFEDTVEFWHNWLSQ
                     CTYQGRWRERVYRSALVLKLLTFATTGAIVAAPTTSLPESLGGPRNWDYRYTWIRDAS
                     FTCYALLSLGFQEEAGRFMGWVEARCRELDPKKGGLQIMYGIDGRHDLKEEILDHLSG
                     YRGSRPVRIGNNAYKQVQMDIYGELMDSVYLYNKYAHPLSYDQWTYLRELMDWVAHHW
                     QTPDSGLWEVRSGKEPFVYSRFMIWVAMDRALRVAFQRGLPCNEDLWKQVRDAAYEEV
                     MAKGWSTERQAFVQYYGSDILDASNLVMPIVKFVGPTDPRMEATIDRTLENLTFDALV
                     YRYDVSRGAKDGLPGQDGTFCLCSFWLVEALTRAGRLKEARLKLEKMFSYANGLGLYA
                     EEISTTGEMLGNYPQAFTHLGLINAAVNLDRALNKSQA"
BASE COUNT          553 a          998 c         1022 g          484 t
ORIGIN      
        1 aggcagcgca cggccagcca ggggatgtag ccgatggcgg ccccgaccag cagcaaccgg
       61 cccgcgccgt tggcccgcca gcccagcgcc agccggcgga aggcataggt cgttgccagc
      121 cccgtgggca gttcggagaa gatcatgtac gagtagctca tcagggggcc gctgaaggcc
      181 agcggcgccc acaccgcgag ggccacccaa cgcttgccgg tcgtctcata ggccagcagg
      241 aagatattcg tcgccagcaa ggcgcccacc aggcacatga agaccaccgt ggtgggccac
      301 cacaacgcga accacgaccc gatgatccag gccggcacca gcaacacccc caggccgggc
      361 gggtggaacg agtacaactc ctggtccggg cgggcgctcg acatggcgag ctggcgcggc
      421 agcgggtacg gcgcgtccat gccgacgaaa tcgggcgggt ggggcgcgag gctgtagaat
      481 ttgtcctcgt cgtggttggc gtagttgttg ctcagttcga ggtcgccatc ctgcaccagg
      541 ctgagcgtgt ccatcaaata gaagggctgg tcgccggtgg gcgggctgct gtagcggatg
      601 atctggggca caaaggcgag gtacacgaga aaggtcagca cgcagagcag cgcgacccag
      661 cgcatcggca ccacccgcgg agccagatcc gccccggcag gcaccgcggg cagcgccgcc
      721 gggctgtccc ggtcgccgcg agtggccccg gcggcatccg gcgcgggcgg gccggggctg
      781 ggccgcgccc ccggcggcac cccggccggc agcggcgtgg ccctggtggc cggctcgccc
      841 ggcgcagcgg aagtctttac gtgatccgtc atgggcgtcc ccttatcgtg gctcagtcgt
      901 gtgcagcacc tcgcaacttc ccgccccctg tcctcgccgt gccccggccc aacaccggcg
      961 gctatcactg atgaccttgc cggtattcta tcccaaagac cggggcgcgg caagcgggta
     1021 gcagcccgaa cggtagccaa tttcacgcga tagacggccc aggcacgtta tagagtacgg
     1081 cggccacgaa ggaggagcaa cggcacatgg cgtacttacc tatcgagaat tacggtgtaa
     1141 tcggcgattg tcatacggtg gccctggtcg gcctcaacgg gtcgatcgac tggttatgct
     1201 tcccggtgtt cgactcgccc agcgtgttcg gcgcgatcct ggacgacgag aagggcgggc
     1261 actttcagat cacgccgctc gaagacgatc tgatggcgcg ccagatgtac ctgccggaga
     1321 ccaacgtgct ggtgacgcgc tttttcagtg ccgccggcct ggccgagatc accgatttca
     1381 tgcccatcga agacgataag cacgccacct ggaaccatcg cgtgatccgc cgggtacgcg
     1441 tggtccacgg gtcgatgcgc ttcaagatgg agtgccggcc cgccttcaac tatgcccgca
     1501 ctaagcacac cgtcatcgcc gaccacgacg gcgtggtgtt cctgtccaag gacctcagcc
     1561 tgggcctggc cagcgacacg ccgatcaaga tcgacgggcc ggcggtctcc agcgagtttt
     1621 ccctgtccgt gggccagacc actgtctttg tgctgcaccg ggtggacgac gacgaaccgt
     1681 gcggtgagta cttgaaagag gacgaggtcg agcagatttt cgaggacacg gtggagttct
     1741 ggcacaactg gctgtcgcag tgtacctatc agggccgctg gcgagaacgg gtctaccgct
     1801 cggcgctggt gctcaagctg ctgacctttg ccaccaccgg ggctatcgtg gccgccccga
     1861 ccacgagcct gcccgaatca ctcggcggcc cgcgcaactg ggactaccgc tacacctgga
     1921 ttcgcgacgc cagcttcacc tgctacgcgc tgctgagcct gggctttcag gaggaggccg
     1981 ggcgcttcat gggttgggtc gaggcgcgct gccgcgaact cgacccgaaa aaaggcggcc
     2041 tgcagatcat gtacggcatc gacgggcggc acgacctcaa ggaagagatc ctggaccacc
     2101 tgtcgggcta ccgcggctcg cgccccgtgc gcatcggcaa caatgcctac aagcaggtgc
     2161 agatggatat ttacggcgaa ctgatggact cggtctacct ctacaacaag tacgcccacc
     2221 cgctgtcgta tgaccagtgg acgtatctcc gcgagttgat ggactgggtg gcccaccact
     2281 ggcagacgcc cgacagcggc ctgtgggaag tgcgcagcgg caaggagccg tttgtctact
     2341 cgcgcttcat gatctgggtg gcgatggacc gggcgctgcg cgtggccttc cagcgcggcc
     2401 tgccgtgcaa cgaagacctg tggaagcagg tgcgcgacgc ggcgtatgag gaggtcatgg
     2461 cgaaaggctg gagcaccgaa cgccaggcgt ttgtgcagta ttacggcagc gacatcctgg
     2521 atgccagcaa cctggtgatg cccatcgtca aatttgtcgg ccccaccgat ccgcgcatgg
     2581 aggcgactat cgaccgcacg ctggagaacc tgaccttcga cgcgctggtc taccgctacg
     2641 atgtgagccg gggcgccaaa gacggcttgc ccggccagga cggcacgttc tgcctgtgca
     2701 gcttctggct ggtcgaggcc ctgacccgcg ccgggcgcct gaaagaagcg cggctcaagc
     2761 tggagaagat gttcagctac gccaacggcc tgggcctcta cgcggaggaa atcagcacga
     2821 ccggcgagat gctgggcaac tacccccagg cgttcacgca cctggggctg atcaacgccg
     2881 ccgtcaacct ggaccgcgcg ctgaacaaga gccaggcgtg acgcccggcg cgggccggcc
     2941 ctgcccggcg ccgcgctaat cgctgatgcc cgattgcgcg acgattttgg tcggggtgac
     3001 gcgcaccagc agttcgtcgt ccacggcgtt gcgcttgccg taggccgcgg ccttgcc
//
LOCUS       DASUYC010000811         2980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1744065, whole genome shotgun sequence.
ACCESSION   DASUYC010000811 DASUYC010000000
VERSION     DASUYC010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1744065"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            296..>2980
                     /locus_tag="VF276_13315"
     CDS             296..>2980
                     /locus_tag="VF276_13315"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HEX6607881.1"
                     /translation="MDAPDSAPVAAPRAASAPLTLYVVSHTHWDREWYLPFQLFRMKL
                     VRLIDKLLAILAADPSYAHYMLDGQTVVLEDYLEVRPERAAELRAHVQQGRVLVGPWY
                     ILPDEFLVSGESLIRNFLRGGRIARDFGGAMPAGYIPDPFGHISQMPQILRGFGIDWA
                     VFWRGSGADVPGSEFTWAAPDGSQVLATRLLHGYANGLPLSFSIAAARRQVAWIRDQQ
                     APRRAADMLLVLNGDDHVPPSADLPAILDELNAEFGEQNIRLVHGTLPQYRVALAPAL
                     DEAALPVHSGEFREAAHAYLLPGVLSTRMWIKQANYAAETLLERWVEPWMALFWALDQ
                     ATGASDWEQTLRIDSAAPLRQTAWKYLLQNQPHDSICGCSVDAVHDEMKTRYAAVEQL
                     GGALRDEALRGVGSFIQTAGPDDSGAQAVIVYNPLGTPRTDAAEVTVEVPASLFDFRL
                     LDDSGAEVAYDITEWRQEQIASFDLDPAGLETTLAMGGDGRIMGMAVLDVYLHRSGEP
                     GVIEVDITVAENGEPNLLAIQSAADAARHLVATEQVQLFRLRGHHVAHGVIRFVARDI
                     PGVGYRTYTLAPGDSGLDLPGAADLDAEPEKSAPESDTPPHPAALYQPEPAAAETPRS
                     RTGRRLHPALMTVAGDDGSRAYVIANEFLQVEADPATGQITVTDKELGQVVRHGNVFV
                     DSGDAGDEYNYCPPPHEAIIEGYDRPPRIDVRRDALGSLMVIEGELALPAWLSEDRQS
                     RSLALAQCAVRCGIRLLDGVRRVDIRTTIDNRAGDHRLRVLFDVPFVTDPSEAEGAFD
                     VVRRPAGPLPGSDTWPESAVATYPQKSFVAVGDGTRGVLLANRGLPEFEVQPSGDGML
                     LALTLLRCVGWLSRDDLATRPGHAGPGVATPGGQTFGVH"
BASE COUNT          474 a         1058 c          990 g          458 t
ORIGIN      
        1 ggccactgtc cttcttttga tggaatcgtt tctatgctct tacggttacg ggtggaaacg
       61 attccgcaag aggaaatgtc cggcatccgt tcacatagta tgcgctctgc aaacgcgcag
      121 agcacgaaga acgagactac aacaaataac cttcgcgtcc ttcgtttctt cgcggtgaac
      181 taacgaacgg agggcgcggg atggccgggc ggcacgcttg ccgcgctccc ggctttgcgg
      241 tacaatgcgc gcgtaatcgc accatcggtt cgtatcccag accaggagga tcgctatgga
      301 tgcaccggac agcgccccgg tcgccgcccc gcgcgccgcg tccgccccgc tgacgctcta
      361 tgtcgtcagc catacccatt gggaccggga gtggtacttg cccttccaac tcttccgcat
      421 gaaactggtg cgcttgatcg ataagctcct ggcgatcctg gcggcggacc cgtcctatgc
      481 ccactatatg ctcgacgggc agacggtggt gctcgaagac tatctggagg tgcgccccga
      541 gcgcgccgcg gaattgcggg cgcacgtgca gcagggccgc gtcctggttg gcccctggta
      601 tatcctacca gacgagttcc tggtcagtgg cgagtcgctc atccgcaact tcttgcgcgg
      661 cgggcgcatc gcgcgggact tcggcggcgc catgcccgcg ggctacatcc ccgacccgtt
      721 cggccatatc agccagatgc cgcagatcct gcgcggcttc ggcatcgatt gggccgtgtt
      781 ctggcgcggc agcggcgccg atgtgcccgg cagcgagttc acctgggccg cgcccgacgg
      841 cagccaggtg ctggctaccc gcctgctgca cggctacgcc aacggcctgc ccctgtcgtt
      901 ttccatcgct gccgcccgcc gccaggtcgc gtggatccgc gatcagcagg caccccggcg
      961 ggccgcggac atgctgctgg tgctgaacgg cgacgaccat gtgccgccga gcgccgatct
     1021 cccggccatc ctggacgaat tgaacgccga atttggcgag caaaacatcc gcctggtgca
     1081 cggcacgctg ccccagtacc gcgtcgcgct ggcgccggcc ctcgacgagg ccgccctgcc
     1141 ggtccatagc ggcgagttcc gcgaagccgc gcacgcctac ctgctgccgg gggtgctctc
     1201 aacccgcatg tggatcaagc aggccaatta tgccgccgag acgctgctgg aacgctgggt
     1261 cgagccgtgg atggcgctgt tctgggcgct ggaccaggcc accggggcga gcgactggga
     1321 acaaacgctg cggatcgact ccgcggcgcc gctgcgccag accgcctgga agtacctgct
     1381 gcagaaccag ccgcacgaca gcatttgcgg ctgctcggtc gatgccgtgc acgacgagat
     1441 gaagacgcgc tatgccgccg tcgaacaact cggcggcgcc ctgcgcgacg aggcgctgcg
     1501 cggggtgggc agcttcatcc agaccgccgg gcccgacgac tccggcgcgc aggcggtgat
     1561 cgtctacaac ccgctcggta cgccgcgcac cgacgccgcc gaggtcacgg tcgaagtgcc
     1621 ggccagcctg ttcgacttcc ggctgctcga cgacagcggc gcggaggtgg catacgatat
     1681 caccgaatgg cggcaggagc agatcgcgtc gttcgacctt gaccccgcgg gcctggaaac
     1741 caccctggcg atgggcggcg acgggcggat catgggcatg gccgtgctcg acgtgtatct
     1801 ccaccgcagc ggcgagcccg gcgtgatcga agtagacatc acggtggccg agaacggcga
     1861 accgaacctg ctcgccatcc agagcgccgc cgacgcggcc cgccacctgg tcgccaccga
     1921 gcaggtgcaa ctcttccgtc tgcgcggcca ccatgtggcg cacggggtca tccgctttgt
     1981 cgcccgcgat ataccgggcg tcggctaccg cacctacacc ctggcgcccg gcgactcggg
     2041 actcgatctg cccggcgcgg cggatctcga cgccgaaccg gagaagagcg ccccggaaag
     2101 cgacacgcca ccgcaccccg ctgctctcta ccagcccgaa cccgccgccg cggagacccc
     2161 gcgcagccgg accgggcgcc ggctgcatcc cgccttgatg acggtcgccg gcgacgacgg
     2221 ctcgcgcgcg tatgtcatcg ccaacgagtt cttgcaggtc gaagccgacc cagcgacggg
     2281 ccagatcacc gtcaccgata aagagctcgg ccaggtggtg cgccacggca acgtctttgt
     2341 cgactccggc gacgcgggcg acgagtacaa ctattgcccg ccgccccacg aggcgatcat
     2401 cgagggctac gaccggccgc cgcgcatcga cgtgcggcgc gatgccctgg gtagcctcat
     2461 ggtgatcgaa ggcgaactgg ccctgcccgc ctggttgagc gaggatcggc agagccgtag
     2521 cctggccctg gcccagtgtg ccgtgcgctg cggaatccgc ctgctggatg gcgtgcgccg
     2581 cgtggacatc cgtaccacta tcgacaaccg cgccggcgac caccggctgc gcgtgctgtt
     2641 cgacgtgccc tttgtgaccg acccctccga ggcggaaggc gccttcgacg tggtgcgccg
     2701 accggcgggg ccgctgccgg gcagcgacac ctggccggaa agcgcggtcg ccacctaccc
     2761 acagaagtcg ttcgtcgccg tgggcgacgg cacgcggggg gtgctgctcg ccaaccgggg
     2821 cctgcccgag tttgaagtgc agccgagcgg cgacggtatg ctgctggcgc tgacgctcct
     2881 gcgctgcgtc ggctggctca gccgcgacga cctggcgacc cgccccggcc acgccggacc
     2941 cggcgtggcg acgcccggcg gccagacctt cggcgtgcat
//
LOCUS       DASUYC010000812         3059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_634042,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000812 DASUYC010000000
VERSION     DASUYC010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634042"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VF276_13320"
     CDS             <1..299
                     /locus_tag="VF276_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607882.1"
                     /translation="ADAQPLIMDPLYSAEELTASGATPVPPGETPPVDAVIVQAYHAA
                     FRTLAWATFAARGCRVVLDGRNILDAETQAAIRAAGMTYLGIGRGGLGIGPARQ"
     gene            464..>3059
                     /locus_tag="VF276_13325"
     CDS             464..>3059
                     /locus_tag="VF276_13325"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HEX6607883.1"
                     /translation="MRRLPLVLLCALALLIALPLTLASNGPYPDPPPPPAPPHAFDLS
                     KLPLVFEPNAGQTDPAVQFLAHGARGRFFFTAEGVVITIDTPVAAPPATSAGPHTAAP
                     PPATRATRLQFLGANRQAALHADDLAQGRVNYLLGDDPAQWHTDLPTYGALSYQGLYP
                     GIDLRYTGTAGQLKGTYTVAPGHDPAAIRWRYQGAGNVTVDSAGNLQVALTPAPAGQL
                     IEQAPVAWQMIGDQRVDVPVRYALTPDGSVGFAFGAYDAAYALIIDPVLTYSTYLGGG
                     GHDYAKRLLVDASGNTYLTGETGSINFPTADPYQSGNNGASDSFVAKLDATGSTVLYA
                     TYLGGSGRDVGTGIGLDSTGNIYVAGFTASTDFPRANALQINYAGGRDDAFFAKLNPT
                     GSSLLYSTYLGGNGYDEVFDMVVDGQGTTYAVGETGSSNFPTANPYQLSLQGWADAFI
                     IKLTASGSSLLYSTYFGGSDGDGAYGLALDQNGNVYLAGTTHSYNLPRMNAYQAQFGG
                     GDSDAFVAEFNPTSGALAYASYLGGSGADIGQDVALDTYDNMYLTGWTASTNFPRANA
                     VQNVYGGGTYDAFLTKLNSGGSTLSYSTYLGGNGEDEASGLAVDNMGDAYLVGYTTST
                     NFPQVNPLQPNYGGGTTFGDAFVTRLNAAGDAVDYSTYLGGAGEDSAWDIKVNGAQDA
                     YVAGITGSTDFPPAGTPAQAANAGGDDAFLVKISNNTGTPTATITPVSPSNTPTPIPT
                     DTPTTTPTRPASPTTATAIPATAPPTPTPTATPTICPVQFNDVGIEHPFYPYIRCLAC
                     RGIISGYDCGATGEPCPGNYFRPYANITRGQAAKIIAISATLTDTIPLDRQTFEDVPR
                     SHPFWLPIE"
BASE COUNT          575 a         1037 c          884 g          563 t
ORIGIN      
        1 cagccgatgc ccaacccttg atcatggacc cactctacag cgcggaggaa ctgaccgcct
       61 ccggcgcgac cccggtcccg cccggcgaga cgccgccggt ggacgccgtg atcgtgcagg
      121 cctaccacgc ggctttccgc accctcgctt gggcaacctt cgccgcgcgg ggctgccggg
      181 tggtgctcga cgggcgcaac atcctcgacg cggagacgca agcggcgatc cgcgcggcgg
      241 gcatgaccta cctgggcatc gggcgcggcg gcctcggcat aggtcccgcc cggcaataaa
      301 cggggccgga tcgccggcgc ctggatgcca gagtactata gcaacccggc agaaaagctg
      361 ttataatagc cccgttcact aatagtccgg ccatactccc ctggtcagcg ggaccacgat
      421 ggtcccgccg tgccctgtcc acgcacttat caaggagatc tcgatgcgcc gtctgcccct
      481 cgtcctgctc tgcgcactcg cactgctcat cgccttaccg ctcacgctgg cctcaaacgg
      541 cccatacccc gacccccctc ctccgcccgc gccgcctcac gctttcgacc tgagcaagct
      601 cccgctcgtc tttgagccca acgccgggca gacggatccg gcggtacaat tcctggccca
      661 cggcgcgcgg ggccgcttct tcttcaccgc cgaaggcgtg gtcatcacca tcgacacgcc
      721 ggtggccgcc ccgcccgcga cgagcgcggg cccgcatacc gccgcgccgc cgcccgcgac
      781 ccgtgccacc cggctgcaat tcctgggcgc caaccgccag gccgcgctgc acgcggacga
      841 tctggctcag gggcgagtca actacctgct gggcgacgat cccgcgcagt ggcacaccga
      901 tctgccgacc tacggagcgc tcagctacca gggcctctat cccggcatcg atctgcgcta
      961 caccgggacg gccggccaac tgaaaggcac ctacaccgtc gcgccgggcc acgatccggc
     1021 ggcgatccgc tggcgctacc agggcgccgg caatgtcacc gtggatagcg cgggcaatct
     1081 ccaggtcgcg ctcacccccg cgcccgccgg ccagttgatc gagcaggcgc cggttgcctg
     1141 gcaaatgatc ggggaccagc gcgtggatgt gccggtgcgt tacgcgctca ccccggacgg
     1201 gagcgtcggg tttgccttcg gcgcttacga cgctgcgtat gcattgatta ttgatccagt
     1261 attgacgtat tcgacctatc tgggtggcgg cggccatgat tacgccaaac ggctgctggt
     1321 cgacgctagc ggaaacacct accttacggg ggagaccggc tcgatcaact ttcccacagc
     1381 cgatccgtat cagtccggca ataacggagc cagcgactcg tttgttgcca agctggatgc
     1441 gaccgggtcc actgtcttgt acgccacata tctgggaggc agcggtagag atgtgggcac
     1501 cggcatcggc ctggacagca ccgggaacat ctatgtagcc ggttttaccg cctccacaga
     1561 cttcccgcgt gccaacgccc tgcaaatcaa ctatgcgggg ggccgggatg acgcattttt
     1621 cgccaagctg aatcctacgg gatcgagcct cctctactct acctaccttg gcgggaatgg
     1681 ttatgacgaa gtcttcgaca tggttgtcga cgggcagggc accacctatg ccgttggcga
     1741 gaccgggtcg tccaacttcc cgaccgccaa cccctatcaa ctgagtctgc agggttgggc
     1801 cgacgcgttc attattaagc tgaccgcctc gggatcaagc ctgctctact cgacctattt
     1861 tggcggcagc gacggcgacg gcgcatatgg actcgccctg gatcaaaacg gcaatgttta
     1921 cctggccggg actacgcact cctacaactt gccgcgaatg aatgcctatc aagcccaatt
     1981 tggtgggggc gacagcgatg cctttgtcgc cgagttcaat ccgaccagcg gcgcactggc
     2041 ctatgccagt tatctaggag gtagtggcgc cgatatcggg caagatgttg cactcgacac
     2101 ctacgataat atgtatctca caggctggac ggcttccacc aatttcccgc gtgctaatgc
     2161 tgtgcagaat gtctatggtg gcggcactta cgacgcgttc ttaacgaaac tgaatagcgg
     2221 ggggtcaacg ctcagttatt cgacctacct gggcggcaac ggggaggatg aggcgtcggg
     2281 cctggcagta gataacatgg gggatgccta cctggtgggt tacactacat ccacgaactt
     2341 cccacaggtc aatccgcttc agccgaatta tggaggcggt acgactttcg gtgatgcctt
     2401 tgtgacaagg ctgaatgccg ccggcgatgc ggtggactac tccacgtatc ttggtggggc
     2461 cggcgaggac tcggcctggg atatcaaagt gaacggcgcc caggatgcct atgtggccgg
     2521 tattaccggt tcgactgatt ttccccccgc cggtacgccg gcccaggctg caaatgcagg
     2581 aggcgatgat gccttcctcg tcaaaatcag caataacacc ggcacaccga cggccacaat
     2641 caccccggta agcccctcaa atacgcccac cccgattccc accgacacgc ccactacgac
     2701 gcctacgcgt cctgcgtcgc cgacgacggc caccgccatc cctgctaccg cgcccccgac
     2761 gcctaccccg acggcaacgc ctaccatttg ccccgtgcag ttcaatgatg tcgggattga
     2821 gcatcctttc tacccgtaca tccgctgttt ggcgtgccgg ggtattatca gcggttatga
     2881 ctgcggggca acaggcgaac cgtgtccggg caactatttc cggccgtatg ccaatatcac
     2941 ccgcgggcaa gcagcgaaga tcatagcgat aagtgctaca cttacagaca cgattcccct
     3001 tgaccggcag acttttgagg acgtgcctcg cagtcatccc ttctggttac cgattgaac
//
LOCUS       DASUYC010000813         3685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_127489,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000813 DASUYC010000000
VERSION     DASUYC010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127489"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1568
                     /locus_tag="VF276_13330"
     CDS             <1..1568
                     /locus_tag="VF276_13330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607884.1"
                     /translation="PARATEAGAQARQLASLYEQGRQAGARADWAAAAAAFAGAADIS
                     PDYQDVAAQLAAARDQLRRTGLAGAVALVEDGPQPGLYLEPDGRYLPGSDGSSRVRAV
                     VGDGRRFIYDQAPRVPPGGSPPALPTPAPRAGFWVPTPSPWQIRPAVLATLSPSGTVT
                     LQPLPDGIDGRGLGMFLGDTLWWYAPRNDFGYYSFTVNYYGLSDGARGMVVGSSGRDE
                     LIAWQPDGRRVLMASYGSVPPGSAPRPRLDVTDARATHLGTLGTVEGTVIDAGFSGDG
                     RYLFFQTASRGSLTQTLYLADLANLAQPRITPIREQVTPGNTSAGMAAVWQGPQPQLL
                     VDTWTAAGEHIFTFDPAIHAFAALWSGPSRPAVQDLATISPDGRQFALRYHTPTGQDN
                     LRVAAPPGTPRDVPVQIDPRDSVDLTFTSDGAHLIYSNYRLDFRPGTRRMPVFSLATT
                     GTAPPARLGERDWDADLQTLALPRAGSLAVLVTPQHELHAVTYDGRTDLLIASGVAAV
                     WSLDTRPGPAWTH"
     gene            1906..>3685
                     /locus_tag="VF276_13335"
     CDS             1906..>3685
                     /locus_tag="VF276_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607885.1"
                     /translation="MKLARWGAALLLLNFALTGCDLFGAATPTPPPPTPAPASDASPT
                     TAPASATQTQPVSGSLGSANLNHLNFLVEDVTIAGKPMAITHIYSEFPKYEWVDASGE
                     GIAALDDAARAAVVYLTAYERRPDPAFLDKARRLLNFAMYLQTDDGEFYNFITDRQGT
                     INKTGNTSYKSAGWWAARGLWAIGYGYRIFKTVDPAYAGELQRHFALGRDAWARQVTP
                     LYTKITPVHDVPVPGWLIDGGADVTAIAVLALLEVDRAENGQDATTRTLLTQLADGLA
                     QYQLGDGINYPWGMHPDSAAAPFSWHAWGSTQSFALARAGKQLGQTDWINSARKEADL
                     FFSRFVAGSMVSEWGVLPFAYPQIAYGVNSITQAFLALYDATGDASYAQRAGLAAAWF
                     FGNNAAQTAMYDPATGRGFDGLSGGNAQRINKNAGAESTIESLMAVLAVEAVPAAAQY
                     LHFKEVQSTPWQVFEAEDGKQLAGDAVAAYRAGDSTGDARWSNGHYVTMKAGDVISMS
                     LTVPADGQYYLYSAYLKQQFYQQPFVAEALRVPTPPTIDGKLDEWQVAQPLSVTQASN
                     ILRGTAGWGGADKDSFVGYLLWDDTNL"
BASE COUNT          590 a         1374 c         1184 g          537 t
ORIGIN      
        1 cccccgcccg cgctacggaa gccggcgccc aggcccggca actggctagt ctctacgagc
       61 agggccgcca ggcaggcgcg cgggcggatt gggccgctgc cgccgccgcg ttcgccggag
      121 ccgcggacat cagcccggac taccaggacg tggcggcaca actcgccgcg gcgcgggacc
      181 aactgcggcg caccggcctg gcaggcgctg tcgccctggt cgaggacggg ccgcagccgg
      241 gcctctacct ggaacccgat gggcgctacc tgccgggcag cgacggcagc agccgggtgc
      301 gggccgtggt cggggacggg cggcggttca tctacgacca ggcgccgcgg gtgccgccgg
      361 gcgggtcgcc gccggccctg cccacgcccg ccccgcgcgc cggtttctgg gtccccaccc
      421 cctcaccctg gcagatccgc cccgccgtgc tggcgaccct gagtccgtcg ggcaccgtga
      481 ccctgcaacc gctgcccgac ggcattgacg ggcgggggtt agggatgttc ctgggcgata
      541 cgctctggtg gtatgccccg cgcaacgatt tcggctacta cagcttcacc gtcaactact
      601 atgggctgag cgatggagcg cgcgggatgg tggtcgggtc gagcgggcgc gacgagttga
      661 tcgcctggca gcccgacggc cgccgggtgc tgatggcaag ctacggcagc gtgccgccag
      721 ggtccgcgcc gcgcccccgg ctcgatgtca ccgatgcccg cgccacgcac ctgggcacgc
      781 tcggcaccgt cgaagggacg gtgatcgatg cgggcttcag cggggacggc cgctacctgt
      841 tcttccagac cgcttcgcgg ggcagcctca cccagaccct ctacctggcc gatctggcaa
      901 atctggccca gccgcgcatc acgccgattc gagagcaggt cacgcccggc aacacgtccg
      961 ccgggatggc cgccgtctgg caagggccgc agccgcagtt gctggtcgat acctggaccg
     1021 ccgcgggcga gcacatattt accttcgacc ccgccatcca cgcctttgcg gcgctctgga
     1081 gcggcccatc ccggccggcc gtccaggatc tggcgacgat cagccccgac gggcggcaat
     1141 tcgccctgcg ctatcacacg cctaccgggc aggacaacct gcgcgtggcc gcgccgccgg
     1201 gcaccccgcg cgacgtgccc gtccagatcg acccgcgcga ctccgtggat ctcaccttta
     1261 cctccgacgg cgcacacctg atctacagca actaccggct cgacttccgc cccggcacgc
     1321 ggcgcatgcc ggtgttcagc ctcgccacga ccgggaccgc gccgcccgcc cgcctgggcg
     1381 agcgcgactg ggacgccgac ctccagaccc tggccctgcc ccgcgccggc agcctggcgg
     1441 tcctcgtcac cccgcaacac gagttgcacg ccgtgaccta cgacgggcgc accgatctgc
     1501 tgatcgcgtc cggggtcgcc gccgtctgga gcctggacac gcgccccggc cccgcctgga
     1561 cgcattagcc tctggcagcc caaagcccac gctgttgatc gtcgccgcgg ctgcggatcg
     1621 gcggcgggcc gggttcaaac cggcccctac accgcggacc gccggctagc cgccctccac
     1681 catgtcccat gctgttccat tggaacgcca tccctgccac tttagctgcc cccatcacta
     1741 ctctaattct cacgccgcag ggtattgact ccggggtcta accgtgctat attgtcctta
     1801 ctcacgtaaa cgcttacgta cttgttacgt tctcgcatac gtcccccacc ccagcaagta
     1861 cctgaaccaa acaccggaat ctgcacggca tctgcgggag gagcgatgaa gctagctcgc
     1921 tggggagcag ccttgctgct gttgaatttc gccctgacag gctgtgacct gttcggcgcg
     1981 gcgacgccca cgccgccacc gcccactccc gcccccgcgt ctgatgcgtc gcccacgacg
     2041 gccccggcga gcgcgaccca aacccagccg gtgagcggat cgctgggcag cgcgaacctg
     2101 aatcatctca actttctggt cgaggatgtc actatcgccg gcaagccgat ggcgatcacc
     2161 cacatctaca gcgagttccc caagtatgag tgggtggacg cctccggcga aggcatcgcc
     2221 gccctggacg acgcggcgcg ggccgcggtg gtctatctca cggcctatga gcggcggccc
     2281 gacccggcgt tcctcgacaa agcgcgccgc ctgctcaact tcgccatgta cctgcaaacc
     2341 gacgacggcg agttctacaa cttcatcacc gaccgccagg gcacgatcaa caagaccggc
     2401 aacaccagct acaaatcggc gggctggtgg gcggcgcgcg gcctgtgggc catcggctac
     2461 ggctaccgca tcttcaaaac cgtcgacccg gcctatgccg gcgagctaca gcgccatttc
     2521 gccctgggac gcgacgcctg ggcacgccag gtgacgccgc tctacaccaa gatcacaccc
     2581 gtccacgacg tgccggtgcc gggctggctg atcgacggcg gcgccgatgt caccgccatt
     2641 gccgtgctgg ccctgctgga ggtggaccgc gcggagaacg gccaggacgc caccacgcgc
     2701 accctgctga cccaactggc cgacgggctg gcgcagtacc agttgggcga cggcatcaat
     2761 tacccgtggg gtatgcaccc ggacagcgcc gcggcgccct tctcgtggca cgcctggggc
     2821 agcacgcaga gtttcgccct ggcccgcgcc gggaagcaac tcggccagac ggactggata
     2881 aactcggcgc gcaaggaagc cgatctcttc ttcagccgct tcgtggccgg gtcgatggtc
     2941 tccgaatggg gcgtgctgcc cttcgcctat ccgcagatcg cctacggcgt gaacagtatc
     3001 acccaggcgt tcctcgcgct ctacgacgcg accggcgatg cgagctacgc ccagcgcgca
     3061 gggctggcgg cggcctggtt cttcggcaac aacgccgcgc aaaccgccat gtacgaccct
     3121 gccaccgggc gcggcttcga cgggctgagc ggcggcaacg cccagcgcat caacaagaac
     3181 gccggcgccg agtcgaccat cgagagcctg atggccgtgc tggcggtgga ggcggtgccc
     3241 gccgccgcgc agtatctgca cttcaaggaa gtccagagca cgccctggca ggtgttcgag
     3301 gccgaggacg gcaagcaact cgcaggcgac gcggtggccg cctaccgggc gggcgacagc
     3361 accggcgacg cgcgctggag caacggccac tatgtaacca tgaaggcggg cgacgtgatc
     3421 agcatgtccc tgaccgtgcc cgccgatggg cagtattacc tctactcggc ctacctcaag
     3481 cagcagtttt accagcagcc gttcgtggcc gaggcgttgc gcgtccccac cccgcccacc
     3541 atcgacggca agctggacga gtggcaggtc gcgcaacccc tgtcggtcac gcaggccagc
     3601 aatatcctgc gcggcacggc gggctggggc ggcgccgaca aggacagctt cgtcggctac
     3661 ctgctgtggg acgacaccaa cctct
//
LOCUS       DASUYC010000814         2278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_128143,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000814 DASUYC010000000
VERSION     DASUYC010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128143"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VF276_13340"
     CDS             complement(<1..258)
                     /locus_tag="VF276_13340"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX6607886.1"
                     /translation="MTALAGTLSDMSMVNLLQVFTVSKKTGILRLLDGDLRGGLYAVA
                     GQVVHAEVLDGDTDRVLISGEEAFYTIMHWANARFWFTPAIE"
     gene            complement(650..2068)
                     /gene="mnmE"
                     /locus_tag="VF276_13345"
     CDS             complement(650..2068)
                     /gene="mnmE"
                     /locus_tag="VF276_13345"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012303294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEX6607887.1"
                     /translation="MYEDTIAAVATPPGTGGIGTIRISGTQALPILRALFVRPSGRPL
                     AAGKPPPAHHLLYGYIRDPATGERIDEVLAAYMPAPHSYTREDVVELDSHGGPAPLQR
                     ILGLTLRQGARPANPGEFTLRAFLHGRLDLAQAEAVLDVINARTQAGLRAAVDQLGGR
                     LSTRVRDFRARLLRVLAHLEAEIDFPEDDVPPSDVTPLLAPLLDEIRALSADAESGML
                     IRQGVRTALIGRPNAGKSSLLNALLRADRAIVTPIPGTTRDTLEETASLGGVPFVLVD
                     TAGITETADLVEQLGIERSRAAAERADLVLLVIDAAQPLTATDRAALHSVLEARGPGG
                     AGTDLRDRLLVVLNKSDLPARVTGDEVAALLPVVPLVHASAATPGGVAALESAMVARV
                     LGGRSASSDVPLVTNVRHRDALLQAADHLAGALNAARAGTPAAFVAVDIHAALDALGT
                     ITGETVGEDLLDVIFREFCIGK"
     gene            complement(2068..>2278)
                     /locus_tag="VF276_13350"
     CDS             complement(2068..>2278)
                     /locus_tag="VF276_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX6607888.1"
                     /translation="TVPSCHAAPVDFTGAGDVFTAAFLIGYRESGDMWQAAAFAHAAA
                     AFAIEGMGTAGIATRTRVEARMAAR"
BASE COUNT          339 a          730 c          844 g          365 t
ORIGIN      
        1 ctcgatcgcg ggcgtgaacc agaagcgggc gttcgcccag tgcatgatcg tgtagaaggc
       61 ttcttcgccg ctgattagca ccctgtccgt gtcgccgtcc agcacctcag catggacaac
      121 ctggcccgcc acggcgtaca agccgccgcg cagatcgccg tccaacagcc gcaagatccc
      181 ggtcttttta ctgaccgtga acacctgcag cagattgacc atgctcatat cgctgagcgt
      241 tcccgctaga gccgtcatgg ttctgcgtcc tcatattcct aactaacggt gaaaagccgg
      301 ggattgggta gcgggcgaac ggcaccggcg gctccggttc gccaccgatc cttaccttag
      361 tttatgcgct gcccggtggc ttgcctatag ccggatggtc cggttgccta taggacctta
      421 gtcatgaaac cggggccggt gtgtcccaag attagtttac cagggcgggc ttacagtggg
      481 gtttaagttg cggctgggcc tgattacagg ccgccaagta ttcgcgcgcc ggattatcct
      541 taagaaaggt taaggtccgc tgaatccggg gccggatttt cggcgcgctt gcgagggttt
      601 gccttacggg cgaaagagga ccgctgtgtg ctcggcggca gccacgctgc tacttgccga
      661 tgcagaactc gcggaagatc acgtcgagca ggtcttcgcc cacggtctcg ccggtgatgg
      721 tgccgagggc gtcgagggct gcgtggatgt ccacggccac gaaagcagct ggagtgccgg
      781 cgcgggccgc attgagggcg ccggccaggt gatcggcggc ttgcagcagc gcgtcgcggt
      841 ggcggacatt cgtcacgagc ggcacatcgc tgctcgcgct gcgtccgccc aggacgcggg
      901 ccaccatggc gctttcgagt gcggccacac cgccgggcgt ggcggcggag gcgtggacca
      961 gcggcacaac cggcagcaag gcggccacct cgtcgccggt gacgcgggcc gggaggtcgc
     1021 ttttgttgag cacgaccagc aagcggtcgc gcaggtcggt gcccgcgccg ccggggccgc
     1081 gggcctccag gacgctgtgc agggcggcgc gatccgtcgc ggtcagcggc tgcgccgcgt
     1141 cgatcaccag gagaaccagg tcggcgcgct cggcggcagc ccggctgcgc tcgatgccca
     1201 gttgctcaac gaggtccgcc gtctcggtaa tgcccgctgt atcgaccagc acgaagggca
     1261 cgccgcccag gctggccgtc tcctccaggg tgtcgcgggt ggtgccgggg atcggcgtga
     1321 cgatggcccg gtcagcgcgc agcagcgcgt tgagcaggct cgatttgccg gcgttgggcc
     1381 gcccgatcaa agccgtgcgc acgccctggc ggatgagcat cccgctctcg gcatcggcgc
     1441 tgagggcgcg gatctcgtcc agcagcgggg ccagcagtgg cgtcacgtcg gacggcggca
     1501 cgtcgtcctc ggggaagtcg atctcggctt cgaggtgggc cagcacgcgc agcagccggg
     1561 cgcggaagtc gcgcacgcgg gtcgagagcc gcccgccgag ttgatcgacg gcggcgcgca
     1621 ggccggcctg ggtgcgcgcg ttgatcacgt cgagcacggc ttcggcctgg gccaggtcca
     1681 gccgcccgtg caggaaggcg cgcagggtga attcgccggg attggccggg cgcgcgccct
     1741 ggcgcagggt caggcccagg atgcgttgca ggggcgcggg gccgccgtgg ctgtcgagtt
     1801 cgaccacatc ttcgcgggtg tagctgtgcg gggcgggcat ataggcggcc agcacttcgt
     1861 cgatccgctc gccggtggcc gggtcgcgga tatagccgta gagcaggtga tgggcggggg
     1921 gcggcttgcc ggcggcgagg ggtcgcccgg agggccgcac aaacagcgcg cgcaagatcg
     1981 gcagcgcctg cgtcccgctg atgcgaatcg tgccgatgcc gcccgtgccc ggtggcgtgg
     2041 cgacggcggc gatcgtgtcc tcgtacatca gcgcgcggcc atgcgggcct cgacccgcgt
     2101 gcgggtggcg atgcccgcgg tgcccatgcc ttcgatggcg aacgccgccg cggcatgggc
     2161 gaaggcggcc gcctgccaca tgtcgcccga ttcccgatag ccgatgagga aggccgccgt
     2221 gaacacatcg cccgcgccgg tgaaatcgac cggggcggca tggcatgagg gcacggtg
//
LOCUS       DASUYC010000815         2804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1308216, whole genome shotgun sequence.
ACCESSION   DASUYC010000815 DASUYC010000000
VERSION     DASUYC010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1308216"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..159)
                     /locus_tag="VF276_13355"
     CDS             complement(<1..159)
                     /locus_tag="VF276_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014694477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX6607889.1"
                     /translation="MDDTRGPVALVLGGGGILGASEVGMLRALLDAGVRPDIVVGTSV
                     GAINGALLA"
     gene            complement(245..2443)
                     /locus_tag="VF276_13360"
     CDS             complement(245..2443)
                     /locus_tag="VF276_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX6607890.1"
                     /translation="MVPTQELLPRLILIAMMATAIGWFGYRLRQIYSYAMLGRPQPRF
                     DRLPERIGLFSKNVLGQFRLWNHYTAAGLAHAITFWGFLIIQIGALDLFLSGLVPGWR
                     LPLFGGTTWFMFVQDLAEVFVLVAIVTFFYRRYVTQPERLSRDPQGPIILALIALLMI
                     TLLIIQGAEIVAGAEPHDWVFRPASTAVAALFEALGATPTSGWVHLLHDASWWVHIGT
                     ILGFLVYLGYSKHMHLFTSPLTVFFYDLTPKGALPLVKDIEQRIENEEPLGASRVEDL
                     GRKDILDLYTCTECGRCQDACPAWATDKPLSPKKLILDLQEHVLMAGPALVAAKQKAP
                     VQQEAAGSGVTALQSLVEEARIPLAGGVIMDETLWSCTTCRACMQACPVFIEHVPKIV
                     DMRRNLVMQEGRIPDELARTFRNLEQNGNPWQFNNATRTKWADGLGIPEVTDVEDVSG
                     LEVVWWVGCLGSFDQRNQKISRALAAIFKQANVKFAIMGKSESCCGDPARRPGNEYLY
                     QVLAQTNIEVLNELKVTKLVTACPHCFNTIKNEYPQLGGQYEVLHHTQLLNRLVREKR
                     LTPVARPGESAQTGAASTGAKVTYHDPCYLGRHNQVYAPPRELLQILPGVEYAEMERS
                     KEKSFCCGAGGARMWMEEKLGTRINTNRTEEALATGADRIAIGCPFCKVMLSDGLNAA
                     IQDGKATDEVEVVDVAQMLLAAVRRSDGGGGTTEAEQPEPQPDPEPAPVA"
     gene            complement(2619..>2804)
                     /locus_tag="VF276_13365"
     CDS             complement(2619..>2804)
                     /locus_tag="VF276_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX6607891.1"
                     /translation="LAVGISGEIQHKVGMQTSGTIVAINNNREAPIMQFADLAVVGDL
                     FKVVPALNAELARRKNS"
BASE COUNT          434 a          897 c          994 g          479 t
ORIGIN      
        1 tgccaggagt gcgccgttga tggctcccac gctggtgccg acgacgatgt cggggcggac
       61 gccggcgtcc aggagggcgc gcagcatccc cacctcgctg gcgccgagga tgcccccgcc
      121 gcccagcacc aacgccaccg gaccgcgcgt gtcgtccatg cgcggagcgt atgccgtcgg
      181 gctgggtgct gggggtggcc gcacccgacg atgtggtcgg gccggcgtat gccggccggc
      241 cgggtcaggc gacgggcgcg ggctccgggt cgggctgggg ctcgggctgc tccgcctccg
      301 tggtcccgcc tccgccgtcg ctgcggcgca cggcggcgag cagcatctgg gcgacgtcga
      361 cgacctcgac ctcgtcggtc gccttgccgt cctggatggc ggcgttgagc ccgtcggaga
      421 gcatcacctt gcagaacggg cagccgatgg cgatgcggtc ggcgcccgtg gcgagggcct
      481 cctcggtgcg gttggtgttg atccgcgtgc cgagcttctc ctccatccac atgcgggcac
      541 cgccggcgcc gcagcagaag gacttctcct tcgaccgctc catctcggcg tactcgacgc
      601 cgggcaggat ctgcagcagc tcgcgcggcg gggcgtagac ctggttgtgg cggcccaggt
      661 agcacgggtc gtggtaggtc accttggcac cggtggaggc ggcgccggtc tgggccgact
      721 cgccggggcg ggcgacaggc gtcaggcgct tctcgcggac gaggcggttg agcagctgcg
      781 tgtggtgcag cacctcgtac tggccaccga gctgggggta ctcgttcttg atcgtgttga
      841 agcagtgggg gcaggccgtg accagcttgg tcactttcag ctcgttcaag acctcgatat
      901 tggtctgggc cagcacctga tagagatatt cgttgccggg gcggcgggcc gggtcgccgc
      961 agcacgactc gctcttgccc atgatggcga acttgacgtt ggcctgcttg aagatggcgg
     1021 ccagcgcgcg gctgatcttc tggttgcgct ggtcgaacga ccccaggcag cccacccacc
     1081 agaccacctc cagccccgac acgtcctcca cgtcggtgac ttcgggaatg cccaggccgt
     1141 cggcccattt ggtgcgggtc gcgttgttga actgccaggg gttgccgttc tgctccaggt
     1201 tgcggaaggt gcgcgccagt tcgtcaggga tgcggccctc ctgcatgacc aggttgcggc
     1261 gcatatcgac gatcttcggc acatgctcga tgaacaccgg gcaggcttgc atgcaggcgc
     1321 ggcaggtggt gcaggaccag agcgtctcgt ccatgatcac gccgcccgcc agcgggatgc
     1381 gcgcctcttc gaccagcgat tgcagggcgg tcacgcccga cccggcggcc tcctgctgca
     1441 cgggcgcctt ttgtttggcg gccaccaggg ccggccccgc catcagcaca tgctcttgca
     1501 ggtcgagaat cagcttcttg ggcgagaggg gcttgtcggt ggcccaggcc ggacaggcgt
     1561 cctggcagcg cccgcactcg gtgcaggtat agaggtccag gatgtccttg cggcccaggt
     1621 cttcgacgcg gctggcgccc agcggctcct cgttctcgat gcgctgctcg atgtccttca
     1681 ccagcggcag ggcgcccttc ggcgtcaggt cgtagaagaa gacggtgagc ggcgaggtga
     1741 agaggtgcat gtgtttggag tagcccaggt agaccaggaa gcccaggatg gtgccgatat
     1801 gcacccacca cgaggcgtcg tgcagcagat ggacccagcc gctggtgggc gtggcgccca
     1861 gcgcctcaaa cagtgccgcc accgcggtcg aggcgggccg gaagacccaa tcgtgcggct
     1921 cggccccggc cacaatctcc gcgccctgga taatgagcag cgtgatcatg agcagggcga
     1981 tcagggccag gatgatgggt ccttgcgggt cgcggctcag gcgctccggc tgggtgacgt
     2041 agcggcggta gaagaaggtg acgatggcga ccagcacgaa aacctcggcc aggtcctgca
     2101 cgaacatgaa ccaggtggtg ccgccgaaca acggcagccg ccagcccggc acgagccccg
     2161 acaggaacag gtcaagcgcg ccgatctgga tgatcaggaa gccccagaag gtgatcgcgt
     2221 gggccagccc ggcggcggtg tagtggttcc agagccggaa ctggcccagc acgttcttgc
     2281 tgaacaggcc gatccgctcc ggcagccggt cgaagcgcgg ctgcgggcgg cccagcatcg
     2341 cgtagctgta gatctgccgc agccggtagc caaaccagcc aatcgccgtc gccatcatgg
     2401 cgatcaggat caggcgcggc aacaactctt gtgtcggtac catgagcctc ctcgacgagt
     2461 tctgcgtgct gagtgctgag ttgtgtcgag tgatcagtga gcttctcgtg tcattctgag
     2521 cggagcgaag aatctcgtct cgttggggaa gcgacgagac tcttcgctcc gctcagagtg
     2581 acaggaacca cgactcagca ctcagcactc agcactcgtt aactgttctt gcggcgggcg
     2641 agttcggcgt tgagggccgg gaccaccttg aacaggtcgc ccacgacggc caggtccgcg
     2701 aactgcatga tcggcgcctc gcggttgttg ttgatggcga cgatggtgcc gctggtctgc
     2761 atgccgacct tgtgctggat ctcgccggag atgcccacgg ccag
//
LOCUS       DASUYC010000816         2300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1659050, whole genome shotgun sequence.
ACCESSION   DASUYC010000816 DASUYC010000000
VERSION     DASUYC010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1659050"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2300
                     /locus_tag="VF276_13370"
     CDS             <1..>2300
                     /locus_tag="VF276_13370"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6607892.1"
                     /translation="GPPGTGKTRLALEVGARLLADFADGVFLIPLAAVTDPDLVLAAV
                     ARTLEVSDAADRPAAAGLAGALQGRRLLLLLDNFEQVLAAAPALVALLLAVPTVKALV
                     TSREALRVRGEKIVPVPPLTLPPRGAEAAPAQLVPTDAVTLFVQRVQDVQIDFALTPA
                     TAPAVVAICRQLDGLPLALELAAAQLATLSLTELRAELEHGLALLDAGLRDLPAHQRT
                     LRRTLAWSYDRLTPAQQWLFRRLGVFAGGFTAAAVGAVFGDPAPAPMRLLEALVRKSL
                     VRRMADADQDPRFTLLETIRAYALDQLDRQGEAAAIRQVHAAYYRDLAESAAPAMGTA
                     QQDAWFDQFEIEHDNFRHALAWALSSDDAATAVRLGAALGRFWCERRHFHEGRRWLDE
                     ILVLGAARPDALHPIWWAGALRWAGGVVYYLGDLDRARDLGEQSLAIYRRLGDILETA
                     ATLNNLGNIAQDQGDLARARILLQESLDLYRATDQTWRFATTLTNLGGLEADAGNFAR
                     AAQLQTESLALFRALGARRDALFCLICLGETALYRGDWAQARALLAESLDLSRELADP
                     RLLAMSQADLGFLALGEGEGDRAAALLEESLARRWELGEQRLLAGNLEGLAALAVTRG
                     QPARAAWLLGVAGGLRIRLGLPVPPADPTHSRTTRTTAEGAMDAAVFAAALAAGWATP
                     LAQAVESLVAAGRPASHGLAAVPTTVDAHVAAGAAELSAEAGAVAWATGQGMPPPQAT
                     DYTLSGPELPAPAGVPAAVPQHTRPV"
BASE COUNT          301 a          777 c          870 g          352 t
ORIGIN      
        1 cggcccgccg ggcaccggca agacccgcct cgcccttgag gtaggggcgc ggctgctggc
       61 cgattttgcg gacggggtct tcctgatccc cctcgccgca gtgaccgatc ccgacctggt
      121 actcgcggcc gtggcgcgca cactggaggt cagcgatgcg gccgaccggc cagcggccgc
      181 gggactggcc ggggccctcc agggtcggcg cctgctgctg ctgctggata actttgagca
      241 ggtgctggcg gcggccccgg ccctggtggc cctcttgctg gccgtgccca ccgtgaaggc
      301 cttagtcacc agccgggagg cgctgcgtgt gcgcggggaa aagatcgtgc ccgtcccgcc
      361 cctgacgctg cctccccgcg gggccgaggc ggcaccggcg cagctggtcc cgacggacgc
      421 ggtgactctg tttgtgcagc gagtgcaaga tgtgcagatc gacttcgcgc tgacgccggc
      481 tacggcccct gccgtggtcg cgatttgccg ccagttggac ggcttgccgt tggccctcga
      541 actggctgcc gcgcaactgg cgacgctctc gctgaccgaa ctccgcgccg aactggaaca
      601 cgggctggcc ttgctggatg cggggctgcg cgacctgccg gcgcaccagc ggacgctgcg
      661 ccggacgctc gcgtggagtt atgatcggct cacgcccgcc cagcagtggc tcttccggcg
      721 gctgggggtg tttgccggag ggttcacggc ggcggctgtc ggcgcggtgt tcggcgaccc
      781 ggcccccgcc ccgatgcgcc tcctggaggc actcgtccgc aagagcctgg tgcgccggat
      841 ggcagacgca gaccaggacc cgcgctttac gttgttggag acgattcgcg cctacgctct
      901 cgaccaactc gaccggcagg gcgaagcggc ggcgatccgg caggtgcacg ccgcttatta
      961 ccgggatctg gccgaatccg cggcgcccgc gatgggcacc gcgcagcagg atgcctggtt
     1021 cgaccagttc gagatcgaac atgacaattt ccgccatgcc ctggcctggg cgctgagcag
     1081 cgacgatgcg gccacggcgg tgcgactggg ggccgcatta ggccggttct ggtgcgaacg
     1141 ccgccatttc cacgagggcc gccgctggtt ggacgagatc ctcgtgctgg gggcggcccg
     1201 gcccgacgcg ctgcacccga tctggtgggc cggcgcgttg cggtgggcgg gcggcgtggt
     1261 ctactatctg ggcgacctgg accgcgcccg ggatctaggc gagcagagcc tggcgatcta
     1321 tcgccgcctg ggcgatatcc tggagacggc ggcgaccctg aacaatttgg gcaacatcgc
     1381 gcaagaccag ggcgatttgg ccagggcgcg gatcctcctt caggagagcc tggatctcta
     1441 tcgcgccacg gatcagacgt ggcgctttgc caccacgctc accaacctgg gcgggctgga
     1501 ggcggatgcg ggcaatttcg cccgcgccgc gcagctccag accgagagtc tggccctgtt
     1561 ccgcgccctt ggggcacgcc gggacgcgct gttctgcttg atctgcctgg gagaaacggc
     1621 gctgtatagg ggggattggg cgcaggcccg cgcgctgctg gctgagagtt tggacctgag
     1681 ccgggaactg gcggacccgc gcctactggc catgagccag gcggatctgg ggttcctggc
     1741 cctgggggag ggcgaggggg atcgcgccgc cgccctgctg gaggagagcc tggcgcggcg
     1801 ctgggaactg ggcgaacagc gcctgctggc ggggaatctg gaagggctgg ccgcgctggc
     1861 cgtgacgcgg gggcagcccg cccgggcggc ctggctcctc ggggtggcgg gcggcttgcg
     1921 tatccggcta ggcctccctg tgccgccggc cgacccgacc cacagccgca cgacccggac
     1981 caccgcagag ggagcaatgg atgccgcggt ttttgcagcg gctctggccg cggggtgggc
     2041 cacgccgctg gcgcaggcgg tggagtcgct ggtcgcggcg ggccgccccg ctagccacgg
     2101 gctcgcggca gtcccgacga ccgttgacgc ccatgtagcg gccggggcgg ctgaactgtc
     2161 ggcggaagca ggggcggtgg cgtgggccac aggccagggg atgccgccgc cgcaggccac
     2221 tgactatacg ttgagcgggc ctgagttgcc cgcgccggca ggagtcccag ccgcggtccc
     2281 acaacacacg cgtccggttt
//
LOCUS       DASUYC010000817         3726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_128811,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000817 DASUYC010000000
VERSION     DASUYC010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128811"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3674)
                     /locus_tag="VF276_13375"
     CDS             complement(<1..3674)
                     /locus_tag="VF276_13375"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX6607893.1"
                     /translation="MNSADQPAGAPHDAGRTAPPAARRRKGNRVFWALLLGVAAVILA
                     GAGVVAGAASAGWSTETPQVLGIAPYPGSRDVAGDTPIAIAFNMPMNQDSVRAHLRID
                     PPTEGAVTWENNTLIFRPQPGYRRGLTYTVSVDAGASSPLFRPLAAPVSAQFKTAGLP
                     SLYRSVPPAGAESVPTDTLVTLQFSQPMAALAALDSRPDPGAQVHITPDAGGRWQWLG
                     STTLAYRGAALRPATRYSVTVDKALADYTGSPLDQDYSFTFTTLRPAVVATRPEAGTE
                     FAGTSDPIVVTFNGPVDRASAEAAFHLAPPAAGRFAWAPDSTVFTYTVSAPLPEDTQI
                     QATLDGVKPGAGDLAQAAPYTWSFQTSPRPQVTGTTPADGAGNVEATNEIVVRYSAPV
                     SNTNEELLAGVRIDPPVKGLNVYSYDQDATLNIFAPLAPSTSYTVTVGAGGRYLGRDG
                     RPVPERTWHFRTARLKPGAEALSNDGIVSYYAGLPTRVYLSGVNVDGFLRLRLWKLSG
                     DELASYMSLPPDRRVQYQPRTGVARTWDLPVTYQLDQRLTLNPVVGLDGASDRLPPGF
                     YYLRVSTPETANSPGTIAGTALIVGNAGLIMKQSQNEILVWAVDMGTGKPLAGRGIRV
                     MAGGEAAGSATTGADGIAQVRGDWSKYKGQPLALLDGGGDSAVVASWWNENIGPWNFD
                     LSIKDRKYINGMAIYTDRAIYRPGQTVFFKGILRDDDDGRYSVPGGPVPVTLSDINGR
                     VIYSETLALSPFGSFAGQYTLPDYAPLGTYYLNCKCDVRYGGSGVTLEVQEYRKPEYL
                     VGVTTDKPSYINGEQINATASADYFFGGPVAGAKVTTRVLTEDYYFTWTDPNGGGWYD
                     FQDPQPLADRRDSFHGEKRSESQATSDAAGKVQITLPADIGNDPLSQVQTIEASVQDS
                     ANQAISSNTQVIVHKGQYYIGLRPTSYLDAAGRPMTIDVQTVTTAGERKGQTAVTVQF
                     YRRVWEQTTEKDETGLDRPTWKPKDTLLGQQQVTTDAQGHTQVSFTPQDAGQYRIAAQ
                     SRDAAGNTITSATYGYATDSAPGAAPVPWQQKNNNVVTLVADKSSYAPGDTAHILVTS
                     PFTQATGLLTIERGHILSRQIVDLRGPAPVIDVPITDGYLPNVYLGLSMVAPAGGAGP
                     MPDFRQGYLALPVSPASKQLAVTITPSSTEAHPRDTITYTVAVHDAAGRPAAAELSLA
                     LVDKAIFSLAGDQTPALMDS"
BASE COUNT          476 a         1176 c         1413 g          661 t
ORIGIN      
        1 aagctgtcca tcagggcggg cgtctggtcg ccggccagtg agaagatcgc tttgtccacc
       61 agggccagcg agagttcggc ggcggcaggg cggcccgccg cgtcgtggac ggccacggtg
      121 taggtgatcg tgtcgcgcgg gtgggcctcg gtgctgctcg gcgtgatggt cacggcgagt
      181 tgcttggaag ctggggagac gggcagggcc aggtagccct gccggaagtc gggcataggg
      241 cccgcgccgc ccgccggggc gaccatgctc aggcccaggt agacgttggg taggtagccg
      301 tcggtgatcg gcacgtcgat caccggggcc gggccgcgca gatcgacaat ctgccggctc
      361 aggatgtggc cgcgctcgat ggtcagcagg cccgtggcct gggtgaacgg cgacgtgacc
      421 aggatgtgtg ccgtgtcgcc gggcgcgtag ctgctcttgt cggccaccag ggtgacgacg
      481 ttgttgttct tctgctgcca cggcaccggc gccgcgcccg gcgcggagtc ggtcgcgtag
      541 ccgtaggtgg cactggtgat ggtgttaccc gcggcgtcgc ggctttgcgc ggcgatgcgg
      601 tactggcccg cgtcctgtgg cgtgaagctg acctgggtgt ggccctgcgc gtcggtggtc
      661 acctgctgct gtccgagcag cgtgtccttc ggcttccagg tggggcggtc gagaccggtc
      721 tcgtcttttt cggtggtctg ctcccagacg cggcggtaga actggacggt gacggccgtc
      781 tgacccttgc gctcgccggc ggtggtcacc gtctgcacgt cgatagtcat cggccggccc
      841 gccgcatcga gatagctcgt cgggcggagg ccgatgtagt actggccctt gtggacgatc
      901 acctgggtgt tgctgctgat cgcctggttg gcgctgtcct gcaccgacgc ttcgatggtc
      961 tgcacctggc tgagcgggtc gttgccgatg tcggcgggca gggtgatctg gactttgccc
     1021 gcggcgtcgc tggtggcctg gctctcgctg cgcttttcgc cgtggaagct gtcgcggcgg
     1081 tcggcgagcg gctgcgggtc ctggaagtcg taccagccgc cgccgttcgg gtcggtccag
     1141 gtgaagtagt agtcctcggt cagcacgcgc gtggtgactt tggctcccgc gaccgggccg
     1201 ccgaagaagt agtcggcgct cgccgtggcg ttgatctgct cgccgttgat gtagctcggc
     1261 ttgtcggtgg tcacaccgac gaggtactca ggcttacgat actcctgtac ctccagcgtg
     1321 acgcccgacc cgccatagcg cacgtcgcac ttgcagttca ggtagtaggt gcccagcggc
     1381 gcgtaatcgg gcagggtata ttgcccggcg aagctgccga acggcgacag cgccagggtc
     1441 tcactgtaga tgacccgtcc gttgatgtcg ctcaatgtga cgggcaccgg cccaccgggc
     1501 acgctgtagc gaccgtcgtc gtcgtcgcgc aggatgccct tgaagaagac ggtctggcca
     1561 ggccggtaga tggcccggtc ggtgtagatc gccatgccgt tgatgtactt gcggtccttg
     1621 atgctcaggt cgaagttcca ggggccgatg ttctcgttcc accaggaggc gaccacggcg
     1681 gagtcgccgc cgccgtcgag cagggcgagg ggctgccctt tgtacttgct ccagtcgccg
     1741 cgcacctggg cgatgccgtc ggcgccggtg gtggcgctgc ccgccgcttc gcccccggcc
     1801 atcacgcgaa tgccgcgccc ggcgagcggc ttccccgtgc ccatatcgac ggcccagacc
     1861 agaatctcgt tctgcgattg cttcatgatc agcccggcgt tgcctacgat cagggccgtg
     1921 cccgcgatgg tgccggggct attggccgtc tccggcgtgg acacacgcag gtaatagaag
     1981 ccgggtggca ggcggtcgct cgcgccgtcc aggcccacta ccgggttcag cgtcaggcgc
     2041 tggtcgagct ggtaagtgac gggcaggtcc caggtgcggg ctacgccggt gcgcggctga
     2101 tattgcacgc ggcggtcggg cggcaggctc atgtaggagg ccagttcgtc gccgctcagc
     2161 ttccagagac gcaggcgcag gaagccgtcc acgttgacgc cgctgagata gacgcgcgtg
     2221 ggcaacccgg catagtagct gacgatgccg tcgttgctca gcgcctcggc gcccggcttg
     2281 aggcgggcgg tgcggaagtg ccaggtgcgc tcgggcaccg ggcgcccgtc gcggcccagg
     2341 tagcggcccc cggcgcccac cgtcactgtg tagctggtcg agggcgccag cggggcgaag
     2401 atgttgagcg tggcgtcctg gtcatacgaa tagacgttga ggcccttgac aggcggatcg
     2461 atgcgcaccc cggccagcag ttcctcgttc gtgttgctga ccggcgcgct gtagcgcacg
     2521 acgatctcgt tggtcgcctc cacgttcccg gcgccgtccg ccggggtggt gccggtgact
     2581 tgcgggcgcg ggctggtctg gaagctccag gtgtagggcg cggcctgggc caggtcgccc
     2641 gcgccgggct tcacgccgtc gagcgtggcc tggatctgcg tgtcctcggg caggggtgcg
     2701 ctgacggtgt aggtgaagac ggtgctgtcg ggcgcccagg cgaagcgccc ggcggcgggc
     2761 ggcgcgagat ggaaggcggc ttccgccgac gcccggtcca ccggcccgtt gaaggtgacg
     2821 acgatggggt cgctggtgcc ggcgaactcg gtgccggctt cggggcgcgt ggcgacgacg
     2881 gcgggccgca gcgtggtgaa ggtgaagctg tagtcctggt cgagcgggct gccggtgtag
     2941 tcggccagcg ctttatcgac agtcacgctg tagcgggtgg cggggcgcag ggccgcgccg
     3001 cggtaggcga gcgtggtgct gcccagccat tgccagcgcc cgcccgcgtc aggcgtgata
     3061 tgcacctggg cgccggggtc gggccggctg tcgagcgcgg ccagggccgc catcggctgg
     3121 ctgaactgca acgtgaccag cgtatcggtc ggcacgctct cggccccggc aggcggcacg
     3181 ctgcggtaga gtgagggcag ccccgccgtc ttgaactgcg cgctgaccgg cgcggccagg
     3241 gggcggaaga gggggctgct ggccccggca tcgaccgaca cggtgtaggt caacccgcgc
     3301 cggtagccgg gctgcgggcg aaagatcagc gtgttgtttt cccaggtcac ggcgccttcc
     3361 gtgggcgggt cgatccgcag gtgggcgcgc acgctgtcct ggttcatcgg catgttgaag
     3421 gcgatagcga tcggcgtgtc cccggccacg tcgcggctgc cggggtacgg cgcgatgccg
     3481 agcacctggg gcgtctcggt gctccacccg gcgcttgcag ccccggccac gaccccggcg
     3541 ccggcgagga tgaccgcggc gacgcccaat aacagggccc aaaatactcg attccctttg
     3601 cgccgccgcg cggcaggcgg ggccgtccgc cccgcgtcgt gtggtgcgcc tgctggctgg
     3661 tctgcgctgt tcatcgcagg tctcctgttt aattcagtat ggatgcgcgg tactgccggc
     3721 aagatc
//
LOCUS       DASUYC010000818         3399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1283291, whole genome shotgun sequence.
ACCESSION   DASUYC010000818 DASUYC010000000
VERSION     DASUYC010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1283291"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="VF276_13380"
     CDS             complement(<1..142)
                     /locus_tag="VF276_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002028191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6607894.1"
                     /translation="MDETHVETRPLGIHHVTAIAGDPQKNVDFYSGLLGLRLVKLTIN
                     FDD"
     gene            complement(379..879)
                     /locus_tag="VF276_13385"
     CDS             complement(379..879)
                     /locus_tag="VF276_13385"
                     /inference="COORDINATES: protein motif:HMM:NF022182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEX6607895.1"
                     /translation="MPTTPEQIDEYFAKLDWPFSKREPTLWDSGYIGENFRVRFIVRL
                     TERWLYVNALLPAKIHADCRPNLYEHLLRLNFVLNGVKLFLDGDDDVTLAMELPASAV
                     SLEDFETALYAVCSNADRHFVELIRLATDPTAVSSQKPGQTPETPATPGVDWSGDGNT
                     PPESQG"
     gene            complement(863..3247)
                     /locus_tag="VF276_13390"
     CDS             complement(863..3247)
                     /locus_tag="VF276_13390"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEX6607896.1"
                     /translation="MFTHFSPQAQAAVDAAQAAARTAHHDWLGLEHLLIGVLRTGRDL
                     PLPAGQAGDGPALASRLAAALPDGSAGAGQIKATAGAGAAIREANKLMAERHDPEVLP
                     LHLLTAVLQGVAAGTDQCAWLEGLGLDSAAWATQGAAALAALPAAPAHPGLPAPADGT
                     RRPDADRAAATPSLDRYGRDLTALARQGALPPLFGRERELRALMEVLCKMTKNNPALV
                     GEPGVGKTALVEGLAQRMAADKVPAQLRGRRLVELDLNSMVAGTRYRGDFEERLKSVL
                     EETQKSRAVLFLDEMHTAIGAGDTEGGGDLANVLKPYLTRGEISVIGATTIAEYRRYI
                     ERDGALERRFQPLMVEEPSAEATLHILERLRPRFAAHYGTVISDDVLRQVVELSRQYL
                     RHRHFPDKAIDVLQLACARAVLDSLPESEAAPPPAEGAEPACIVTGAHVRAVVADLAG
                     IPLDHFAGNPDLAARYLAMEEILGESVFGQDAAITAVANALRLAKRQLDLNPLRPDGV
                     FLFLGPPGVGKTELGRALGRFLFGDERHVIRLDMSEFSEPHSVSRLVGSPPGYVGHDE
                     GGQLTERIRSRPFSLLLLDDLEKAHPAVINLFVQVFDEGRLTDAHGRTVYFSDTTVVL
                     TSNAGSERYAGRSVGFKTGRPLDPGKRARPPSEEAALAEARKHFPSELISRIDKVILF
                     QPLDRTAARRIVETKLAATLARVSHADGPDGVARPIRVTYDPAVVDYVVDRGFSAEWG
                     ARHVERAIEDEILAPLARLTFRVDWPTVAAVHIHMGPDGLHLDTTEGDYHADHA"
BASE COUNT          492 a         1193 c         1189 g          525 t
ORIGIN      
        1 ggtcgtcgaa attgatcgtg agcttgacca ggcgcaagcc cagcagcccg gaataaaagt
       61 ccacgttctt ttgcggatca ccggcgatag ccgtcacatg gtgaatcccc aggggccggg
      121 tctcgacatg ggtctcgtcc atctggcacc tcctgccgcg ctagccatgg ctgtgtgatc
      181 aacggcaacg ccttcgcgga cgcatggggt acgcatacag gggcgccgcc tggttcgcca
      241 ccctccggca agcaggctgc cggtatgttc agtatacgcc cctgcggagg cgtcgtcaaa
      301 gccgtccggc cccgcacgcg gcgggctgcc tgcgcctgcc cgctccctgg tggtcccggt
      361 gctggccggg accgggcgct agccctggga ctcgggcggc gtgttgccgt cgccgctcca
      421 gtccacgccg ggagtggccg gcgtctccgg ggtctggccc ggcttctgcg acgagacggc
      481 ggtggggtcg gtcgccaggc ggatcagttc cacgaagtgg cggtcggcgt tcgagcagac
      541 cgcgtagagc gcggtctcga aatcctccag cgacaccgcc gaggccggca gttccatcgc
      601 cagggtcacg tcgtcgtcgc cgtcgaggaa cagcttgacg ccgttgagca cgaagttgag
      661 ccgcagcagg tgctcgtaga ggttggggcg gcagtcggcg tggatcttgg ccggcagcag
      721 tgcgttgacg tagagccagc gctcggtcag gcgcacgatg aagcgcacgc ggaaattctc
      781 gccgatatag ccgctgtccc acagggtggg ttcgcgcttg ctgaagggcc agtcgagttt
      841 ggcgaaatac tcgtcgattt gctcaggcgt ggtcggcatg gtagtctcct tcggtagtgt
      901 ctagatgcag cccgtcgggc cccatgtgga tatgcacggc cgccacggtg ggccaatcca
      961 cgcggaacgt caggcgggcc agcggagcca ggatctcgtc ttctatcgcg cgttccacgt
     1021 ggcgcgcgcc ccactccgcg ctgaagccgc ggtccaccac gtaatcgacg accgccggat
     1081 catacgtcac ccggatgggg cgggcgacgc cgtcggggcc gtcggcatgg gagacgcggg
     1141 ccagcgtggc ggccagtttc gtctcgacga tgcgccgggc cgccgtccgg tccagcggct
     1201 ggaacagaat caccttgtcg atgcggctga tcagttcgct cggaaagtgc ttgcgcgcct
     1261 cggccagcgc ggcttcctcc gaaggcgggc gggcgcgctt accgggatcg agcggccgcc
     1321 ccgtcttgaa gcccaccgag cggcccgcgt agcgctcgga ccccgcgttc gaggtcagca
     1381 ccacggtcgt gtcgctgaaa tagaccgtgc gcccgtgcgc gtcggtcagc cgcccctcgt
     1441 cgaacacctg cacgaacagg ttgatcacgg cggggtgcgc cttctccaga tcatcaagca
     1501 gcagcagact gaacggccgg ctgcggatgc gttcggtcaa ctgcccgccc tcgtcgtgcc
     1561 cgacatagcc cggcggcgag ccgaccagcc gcgacacgct gtgcggctcg ctgaactcgc
     1621 tcatgtccag gcggatgaca tgccgctcgt cgccgaacag gaagcggccc agcgcccggc
     1681 ccaactcggt cttgcccacg ccgggcggcc ccaggaacag gaacacgccg tcgggccgca
     1741 gcgggttcag gtccaactgg cgcttcgcca gccgcagcgc gttggcgacg gcagtgatcg
     1801 ccgcgtcctg cccgaacacg ctctcgccca ggatctcttc catcgcgagg tagcgggcgg
     1861 ccaggtcggg gttgcccgcg aagtggtcca gcgggattcc cgccagatcg gccaccacag
     1921 cgcgcacgtg cgccccggtg acgatgcagg ccggttcggc cccctcggca ggcggcggcg
     1981 cggcctcgga ctccggcagg ctgtcgagca cggcccgcgc gcaggccagt tgcagcacgt
     2041 cgatggcctt gtcggggaag tggcgatggc gcaggtactg gcggctcaac tccaccacct
     2101 gccgcagcac gtcgtcgctg atcaccgtgc cgtagtgggc ggcgaagcgc gggcgcagcc
     2161 gctccaggat gtgcagcgtg gcctccgccg acggttcctc caccatcagg ggctggaagc
     2221 gccgctccag cgccccgtcg cgctcgatat agcggcggta ctcggcgatg gtcgtcgccc
     2281 cgatgacgct gatctcgccg cgcgtcagat agggcttgag cacgttcgcc aggtcgccgc
     2341 cgccctcggt gtcgcccgcg ccgatagccg tgtgcatctc gtcgaggaac agcacggcgc
     2401 gcgatttctg cgtctcctcc agcacgctct tgagccgttc ctcgaaatcg ccccgatagc
     2461 gcgtgcccgc caccatggag ttgaggtcga gttcgaccag gcggcgcccg cgcaactgcg
     2521 ccggcacctt gtcggcggcc atgcgctggg ccagcccttc gaccagcgcc gtcttgccca
     2581 cgcccggctc gcccaccagc gccgggttgt tcttggtcat cttgcagagc acttccatca
     2641 gcgcgcggag ttcgcgctcg cgcccgaaca gcggcggcag ggcaccctgg cgggccagcg
     2701 cggtcaggtc acggccatag cggtcgaggc tgggcgtggc cgcggcacgg tcggcgtcgg
     2761 ggcggcgcgt gccgtcggcg ggggccggca ggccggggtg cgcgggcgcc gcgggcaacg
     2821 cggccagcgc cgccgcccct tgcgtggccc acgccgcgct gtccagcccc agcccttcca
     2881 gccaggcgca ctgatccgtg cccgccgcca cgccctgcaa caccgcggtc agtagatgca
     2941 gcggcagcac ctcggggtcg tggcgctcgg ccatcagctt attggcctcg cggatggcgg
     3001 cgccagcccc ggcagtggcc ttgatctggc ccgcccccgc gctgccgtcc ggcaaggcgg
     3061 cggccaaccg cgaggccagc gccggcccgt cgcccgcctg cccggcgggc agcggcaggt
     3121 cgcggcccgt gcgcagcacg ccgatcagca aatgttcgag gcccagccag tcgtgatggg
     3181 cggtgcgcgc ggcggcctgc gccgcgtcca cggcagcctg ggcctgcggc gagaagtggg
     3241 taaacatggt catagtccgc ggcggaccgg gtgcgcgggc gtccagagtg ggaacatagc
     3301 ccattgtaca agagccgtcc gcgccgtgtc aatccggccc tgcttctgct tatgatcacc
     3361 gcagagacgc cgagaacgca gaggatttta aaattagtg
//
LOCUS       DASUYC010000819         2092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_2019053, whole genome shotgun sequence.
ACCESSION   DASUYC010000819 DASUYC010000000
VERSION     DASUYC010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2019053"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VF276_13395"
     CDS             <1..348
                     /locus_tag="VF276_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607897.1"
                     /translation="RWVLAQALGRDLAPGEQACHACNRRACLSTEPGHLYAGTQKQNE
                     ADKARPVNPDVVHILGSAPRPSHAGQVPVIRARHAAGMPIKRIARELDMPRSTVADIV
                     YYRTWKRMEEQAS"
     gene            345..1355
                     /locus_tag="VF276_13400"
     CDS             345..1355
                     /locus_tag="VF276_13400"
                     /inference="COORDINATES: protein motif:HMM:NF024870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6607898.1"
                     /translation="MSSLFQPAAKTQRYLKVLLHGVPGTGKTVAALSFPGPLAVIDLE
                     GGTALYDGDWAVLRTKDLRKIEAAIDELGRSQTYHTVVVDPLTVLWQVLQDAGQGLVD
                     KRRGDSLESGQALSPREWGIIKRRYNSILTRLINLPLHVVLIARDTDLYEGKGDALTK
                     VGTKADVEKSTAYVADFVLWCQATGGEGRAQFSAVVEKSRSPLLPIGQRLTGYAADPT
                     TGLYAKHFAAIAGAHTTGQRVALDEDAAVHDANTAMLEADMQPTHPPPQTRPTVDPAL
                     VAAGNHYRKMAAQAGLATREEQDAFAKQKYPDRGLSTLTIAEWNKLAADCRSMFAPDE
                     AA"
     gene            1410..1739
                     /locus_tag="VF276_13405"
     CDS             1410..1739
                     /locus_tag="VF276_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607899.1"
                     /translation="MLETTTLTAAIRQQRVLADVAQERDFQDRKWGPQDHTPLEWLSI
                     LGEEYGEACQAANKAYWGGKPWAAYRAELVQVAAVAVAMIENLDQRERDNRPYEAGGY
                     YAQEAQS"
     gene            1736..1984
                     /locus_tag="VF276_13410"
     CDS             1736..1984
                     /locus_tag="VF276_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607900.1"
                     /translation="MSARGPAPTQPENELERTCAPVERAAWEAAIDRYQDYRHRLLTR
                     AAPTTASEGALLARYRAQEQAAWDAYKAACRRAQGVAA"
BASE COUNT          410 a          693 c          690 g          299 t
ORIGIN      
        1 cgctgggtgc tggcgcaagc gctggggcgc gatctggcac ccggcgagca agcctgccac
       61 gcctgcaacc gccgggcctg cctgagtacc gaacccggcc acctgtatgc cggcacgcaa
      121 aagcagaacg aagccgacaa agcgcgcccc gtcaatcccg acgtggtgca tatcctgggt
      181 agcgccccgc gcccctcgca cgccgggcag gtgccggtca ttcgggcacg gcacgcggcg
      241 ggaatgccga tcaagcgcat cgcccgcgag ttggacatgc cccggtctac cgtggcggat
      301 attgtctact accgcacctg gaaacgcatg gaggaacaag catcatgagc agtctattcc
      361 aacccgccgc caagacgcag cgctatctaa aggtgctgct ccacggcgtc cccggcaccg
      421 gcaagacggt agccgcctta tccttccccg gaccgttggc cgtcatcgac ctggagggcg
      481 gcaccgcgct ctatgatggg gattgggccg tcctgcgcac caaggatctg cgcaagattg
      541 aggcagcgat tgacgaactg gggcgcagcc aaacgtatca cacggtcgtg gtcgatccgc
      601 tcaccgtgct atggcaggtg ctacaggacg ccggacaggg attagtcgat aagcgccggg
      661 gcgatagcct ggaaagcggg caagccctca gcccgcgcga gtggggcatc atcaagcgcc
      721 gctataacag catcctgacc cggctgatca atctgccgct gcatgtggtc ctgattgccc
      781 gcgacacgga tctgtatgag ggcaagggcg atgcgctcac caaggtgggc acgaaggccg
      841 atgtggagaa atcgacggcg tatgtcgcgg atttcgtgct gtggtgccag gcgacgggcg
      901 gcgagggccg ggcgcagttt agcgcggtgg tggagaaatc ccgctcgccg ctgctcccca
      961 tcgggcaacg cctgacggga tatgccgccg accccacgac cgggctgtat gccaagcact
     1021 tcgccgccat cgccggggcg catacgaccg ggcagcgcgt ggcgctggac gaggatgccg
     1081 ccgtgcatga tgcgaacacc gctatgctgg aggcggatat gcaaccgacg catccgcccc
     1141 cccaaacgcg ccccacggtt gatcccgcgc tggtagccgc cggcaatcac tatcgcaaaa
     1201 tggccgcaca agccgggctg gcgacccgcg aggaacagga tgccttcgcc aagcagaaat
     1261 acccggatcg cggtctcagt accctgacta tcgcagagtg gaacaaactc gccgccgatt
     1321 gccggagcat gttcgccccg gacgaagccg cctaacgtta gcacgcgggg cgggcacatc
     1381 acccgccccg gccttactag gagacaccga tgctagaaac taccaccctt accgccgcca
     1441 tccgtcagca gcgcgtcctg gccgatgtgg cccaagagcg cgattttcag gaccgcaagt
     1501 ggggaccgca ggatcatacc ccgctggaat ggctcagtat tctaggcgag gaatacggcg
     1561 aagcgtgcca ggccgccaac aaagcctact ggggcggcaa gccgtgggcg gcgtatcgcg
     1621 ccgaactggt acaggtcgct gccgtggccg tggctatgat tgagaacctg gaccaacggg
     1681 agcgcgacaa ccgcccctac gaagccgggg gttactacgc acaagaggcg caatcgtgag
     1741 cgcccgtggc cccgccccta cccagccgga gaacgaacta gagcgcacct gcgccccggt
     1801 agaacgcgct gcctgggaag cagccattga ccgctatcag gactaccgcc accggctgct
     1861 gacccgcgcc gccccgacca ccgcgagcga aggggcgctg ctggcgcggt atcgggcgca
     1921 ggagcaagcc gcctgggacg cctacaaagc cgcgtgccgg cgggcgcagg gggtggcggc
     1981 atgaccgaaa ccggcgtcac ctattttagc ggtggggggg ctggccgcgc tcggcatgat
     2041 gcaggccggc gtgcaactgg tcggcgcggt ggaatatgat gccgccattg cc
//
LOCUS       DASUYC010000820         2026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1550722, whole genome shotgun sequence.
ACCESSION   DASUYC010000820 DASUYC010000000
VERSION     DASUYC010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1550722"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..834)
                     /locus_tag="VF276_13415"
     CDS             complement(91..834)
                     /locus_tag="VF276_13415"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX6607901.1"
                     /translation="MQSRARGAPVRRPWWKWAAGRALKRGLAWRYRHFRPDSEPPRWA
                     RVAGLRLYVDRAVFNPGMHFTSGFLAGYLGRPGTMPAGGRVLDMGTGSGVGAIAAARA
                     GAGRVVAVDINPAAVACAAGNVRRYALQDCVDVRAGDLFAPVAGERFDLILCNPPYFR
                     GTPRTVADAAFLGGPQYEWLDRFAAEAGAHLAPGGRILVVLGDAADVPAILARLAAPG
                     WRIAEVARRDIWMEMLYIYALTPRPEAAG"
     gene            1045..>2026
                     /locus_tag="VF276_13420"
     CDS             1045..>2026
                     /locus_tag="VF276_13420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6607902.1"
                     /translation="MLMAAAGSRVGGLETIAREFAAALAARGHTVTLVTGTGPGARLH
                     PDLRGGRAPYRVRTVPMLGQGSWPARLLGRMRGVHPSIVEARTFWAVARRRPAIRREL
                     EGSQVVAAFFEVEAVAASRELAVPVVYYYPGAIDARRLARGRFARMVAISRMVADHYA
                     GMRAGMDLPPIDGVVPPGIRGAWIAPGPAPGVTTSPPEAIFSGRLDWPGPKRAEKLVE
                     WWPQVQAAVPGARLTLIGDGTQRAALRQAVADAGLADTVHLPGALPHDALLARLQAAS
                     LFVFPSQFETFGIAPLEALAAGLPVLASDISALRESLGDSAWLLPVDDDTA"
BASE COUNT          282 a          735 c          741 g          268 t
ORIGIN      
        1 cgtcgaacca gatgccgtag ccggtcagcc cgtagagccg gatcgccagc cccaccagca
       61 taatcgccgc cagcgccgca cccgcgcgac tcatcccgcg gcctccgggc ggggcgtgag
      121 cgcgtagatg taaagcatct ccatccagat gtcgcgacgg gcgacctcgg cgatgcgcca
      181 gccgggggcg gcaagccggg ccaggatggc gggcacatcg gcggcatcgc ccagcacgac
      241 caggatacgc ccgcccggcg cgaggtgcgc gcccgcctcc gccgcgaagc ggtccagcca
      301 ctcatactgc gggccgccga ggaaagcggc gtcggccact gtacgcggcg tgccccggaa
      361 ataaggcgga ttgcagagga tcaggtcgaa ccgttcgccc gcgaccgggg cgaacaggtc
      421 gcccgcgcgc acgtccacgc agtcctggag cgcgtagcgg cggacgttgc ccgccgcgca
      481 ggccacggcg gcgggattga tgtccacggc gaccacccgc ccggcccccg ctcgcgcggc
      541 ggcgatggcg cccacgccgc tgcccgtgcc catgtcgagc acgcgcccgc ccgccggcat
      601 cgtgccgggc cgccccaggt aaccggccag gaagccgctg gtgaagtgca tgcccggatt
      661 gaacaccgcc cgatccacat acaggcgcag cccggctacc cgcgcccagc gcggcggctc
      721 gctgtcgggc cggaagtgcc ggtagcgcca ggccagcccg cgcttcagcg cccgccccgc
      781 cgcccacttc caccagggcc gccgaaccgg cgcgcccctc gctctgctct gcatacgcgc
      841 gatgatacgg cacggggcgc tcgcggtcaa gtgaggtgtc agccgtcagc cgtcagccgt
      901 caggagacgg aaagatacca tgcgagttgc atgacctagg cctattataa tcaccacctg
      961 atcactcgtc tcctgatagc tgacggctga tagctgttga cacgcccccc agcgtcccgt
     1021 aaactcgcgg ctatgcgagt ggcgatgctg atggcggcgg cgggcagccg cgtgggcggg
     1081 ctggagacga ttgcgcgcga gttcgcggcg gcgctagcgg cgcgcgggca tacggtgacg
     1141 ctggtaacgg ggaccgggcc gggcgcgcgg ttgcatcccg atctgcgcgg cgggcgggcg
     1201 ccgtaccggg tgcgcactgt gccgatgcta gggcagggat cgtggcccgc gcggctgctg
     1261 ggccggatgc gcggtgttca cccgtccatc gtcgaggcgc gcaccttctg ggcggtggcc
     1321 cggcgccggc ccgcgatccg ccgtgagcta gagggaagcc aggtcgtcgc cgcgttcttc
     1381 gaggtcgagg ccgtggctgc ctcgcgcgaa ctggccgtgc cggtcgtcta ctactatccg
     1441 ggcgccatcg atgcccggcg gctggcgcgg ggccgcttcg cgcgcatggt cgccatcagc
     1501 cgcatggtgg cggatcatta cgccgggatg cgggccggga tggatctacc gccaatcgac
     1561 ggcgtggtgc cgcccggcat ccgcggcgcg tggattgccc ccggcccggc accgggcgtg
     1621 accaccagcc cgccggaggc catcttcagc gggcggctcg actggccggg gccgaagcgg
     1681 gccgaaaagc tggtggagtg gtggccgcag gtacaggccg ccgtgccggg cgcccggctc
     1741 acgctgatcg gcgacggcac gcagcgagcg gcgttgcggc aggccgtcgc agacgccggg
     1801 ctagcggaca cggtgcactt gcccggcgcc ttgccgcacg acgcgctcct ggcccggctg
     1861 caagcggcga gcctgtttgt cttcccgtcg cagttcgaga ccttcggcat cgcgccgctg
     1921 gaggcgctgg cggcgggcct gccggtgctg gcctccgaca tctcggcgct acgcgagagc
     1981 ctgggcgact ccgcctggct gctgccggtg gacgacgaca cggcct
//
LOCUS       DASUYC010000821         3050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_311360,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000821 DASUYC010000000
VERSION     DASUYC010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311360"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="VF276_13425"
     CDS             <1..698
                     /locus_tag="VF276_13425"
                     /inference="COORDINATES: protein motif:HMM:NF014344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEX6607903.1"
                     /translation="RPDLLLVGASERTSNEGIDMLARELRRRGSPVRTIYAVIMPKQR
                     STMHLDTIFTFLSRDECLVYPPLILPGGGEQVGIVRLSRDVRGVLRAEPEHRSLIDCL
                     QSELGRPIHAIPCGGRSRLYQDREQWTDGANAFALRPGLIMVYERNEQTFGALRAAGY
                     QICRQEDLVRWEPDAGAPAGGHWHENTRLITEIRDHPERKYAIQISGLELSRARGGPR
                     CMTMPLERAPYDM"
     gene            742..1689
                     /gene="arcC"
                     /locus_tag="VF276_13430"
     CDS             742..1689
                     /gene="arcC"
                     /locus_tag="VF276_13430"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEX6607904.1"
                     /translation="MTETLVIALGGNAIKAAGEQGTSTEQFRNVQRAAEAVAELVAGG
                     YRVVVTHGNGPQVGNLLIQQAAGAAQVPAMPMDVAGAMTQGWIGYMIQQSLRNALLRR
                     NHPAATRIATVVTQVIVDRDDPAFENPSKPVGPFYNGDEAQRMRYAGMRLVEDSGRGW
                     RRVVASPQPRAIAEIDAICTMVNDGMLVVAAGGGGIPVVQEADGTLVGVEAVIDKDLA
                     GALLARQVGASRLLILTDVEKVALNYKKPDQVDLAHLTVAEARGYLADGYFARGSMGP
                     KVQAAVNFVEGGGRQAIITQLFSALPALAGETGTVITAE"
     gene            1703..2002
                     /locus_tag="VF276_13435"
     CDS             1703..2002
                     /locus_tag="VF276_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX6607905.1"
                     /translation="MKHFLIEITYQAPLEEIDKVTPEHRAFLQKGYDSGLLLYSGRQV
                     PPAGAVILARAQSQEEVEMFFAGDPYRIGGMGNYRFVEFGPSKFQPFLQGWIEGK"
     gene            2190..>3050
                     /gene="argF"
                     /locus_tag="VF276_13440"
     CDS             2190..>3050
                     /gene="argF"
                     /locus_tag="VF276_13440"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013905327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX6607906.1"
                     /translation="MAVSMKGRSITSMNDLTTEEIWRILELARDLKLKVKTGQPHPVL
                     AGKSLAMIFEKPSARTRVSFEVGMTHLGGHALYLGPNDIGLGKRESVADVARVLGRMV
                     DGIMARTFEQAKVDDLAKYAGVPVINGLSDEEHPCQILADLLTIWEKRNRLEGLHIVW
                     IGDGNNVTHSLMIGAAKMGVNMTVITPENFDVQPKYRDLAEAAARATGARLEFGRDPA
                     AVAGADVIFTDTWASWGQEAEAEARRPIFRPYQVNTALLERAGPQALVMHCLPAHRGE
                     EITDEVIDGPR"
BASE COUNT          529 a         1058 c         1040 g          423 t
ORIGIN      
        1 tgcggcccga tctgctgctg gtgggggcca gcgagcgcac ctcgaacgag ggcatcgaca
       61 tgctggcgcg cgagttgcgc cgccggggca gcccggtgcg caccatctac gccgtgatca
      121 tgcccaagca gcgcagcacc atgcacctgg acactatctt caccttcctc agccgcgacg
      181 agtgcctggt ctatccgccg ctcatcttgc ccggcggcgg cgaacaggtc ggcatcgtgc
      241 gcctcagccg cgatgtccgc ggtgtgctgc gcgccgagcc cgagcaccgc tcgctgatcg
      301 actgcctgca atcggaactg ggccgcccga tccacgccat tccctgcggg ggccgcagcc
      361 ggctctacca ggatcgcgag caatggaccg acggcgcgaa cgcctttgcc ctgcggcccg
      421 gcctgatcat ggtctacgag cgcaacgagc agaccttcgg cgccctgcgc gccgccgggt
      481 atcagatctg ccgccaggaa gacctggtgc gctgggaacc cgacgccggc gcccccgcgg
      541 gcggccactg gcacgagaac acgcggctca tcaccgagat ccgcgaccac cccgaacgca
      601 aatatgcgat ccagatcagc gggctggaac tgtcgcgcgc ccgcggcggc ccccgctgca
      661 tgaccatgcc tcttgagcgt gccccctatg acatgtagtc ccgtgctgcc ccgcgatgag
      721 gagtttatga cagagcaggc catcactgaa acgctggtca tcgccctcgg cggcaacgcc
      781 atcaaggcgg cgggcgagca aggcaccagc accgaacaat tccgcaacgt gcagcgcgcc
      841 gctgaggccg tggccgaact ggtcgccggg ggctaccgcg tggtcgtcac ccacggcaac
      901 gggccgcagg tgggcaatct gctcatccag caggcggcgg gcgccgcgca ggtgccggcc
      961 atgcccatgg atgtggccgg ggccatgacc cagggctgga tcggctacat gatccagcaa
     1021 tcgctgcgca acgccctgct gcgccgcaac cacccggcgg ccacgcgcat cgccaccgtg
     1081 gtcacgcagg tgatcgtgga ccgcgacgac ccggcgttcg agaacccgtc caagccggtc
     1141 ggccccttct acaatggcga tgaggcccag cgcatgcgct acgccgggat gcgcctggtc
     1201 gaggacagcg ggcggggctg gcggcgcgtg gtcgcctcgc cgcagccgcg cgccatcgcc
     1261 gagatcgacg ccatctgcac catggtcaac gacggcatgc tggtcgtggc cgccgggggc
     1321 gggggcatcc ccgtggtgca ggaggccgac ggcacgctgg tcggcgtcga ggcggtgatc
     1381 gacaaggacc tggccggggc gttgctggcc cggcaggtgg gggcctcgcg cttgctcatc
     1441 ctgaccgatg tggagaaagt cgcgcttaac tacaagaagc ccgaccaggt ggatctggcc
     1501 catctcaccg tggccgaggc gcgcggctat ctcgccgacg gctatttcgc ccgcggcagc
     1561 atggggccga aggtccaggc cgcggtgaat tttgtcgagg gcgggggccg ccaggccatc
     1621 atcacgcaac tgttcagcgc gctgccggcc ctggcgggcg agaccggcac cgtgatcacc
     1681 gcggaataag gaggagacat cgatgaagca ctttctgatc gaaatcacct atcaggcgcc
     1741 cctggaggag atcgacaaag ttacgccgga acaccgcgcc ttcttgcaga agggctatga
     1801 cagcggcctt ctgctctact cgggccgcca ggtgccgccc gccggggccg tgatcctggc
     1861 ccgcgcccaa tcgcaggaag aggtggagat gttcttcgcg ggcgacccct accgcatcgg
     1921 cggcatgggc aactatcgct tcgtcgaatt cggccccagc aagttccagc ccttcctgca
     1981 aggctggatc gagggcaagt aacgcctggc gggcctaacc cccaacccct tcccggcgcg
     2041 ggaagggggg cgacgggaca gtgatgtcgt aggggcggac cggcgtggcc gccctgccgc
     2101 cccaccggga tgcggccccg cgccgcgcat ggactcgcat cggcacgaag aaatacccac
     2161 ccgacacata gaaggagccg cataacagca tggcagtgag catgaagggc cggtccatca
     2221 cctcgatgaa cgacctgacg accgaggaaa tctggcggat tctggagctg gcccgcgacc
     2281 tgaagctgaa ggtgaagacc ggccagccgc acccggtgct cgccggcaag agcctggcga
     2341 tgatcttcga gaagccctcg gcgcgcaccc gcgtgtcatt cgaggtgggc atgacccacc
     2401 tgggcgggca cgcgctctac ctcggcccca acgacatcgg cctgggcaag cgcgagagcg
     2461 tggccgacgt ggcccgcgtg ctgggccgca tggtcgacgg catcatggcc cggacgtttg
     2521 agcaggccaa ggtggacgac ctggcgaagt acgcgggtgt gcccgtcatc aacgggctca
     2581 gcgatgagga gcacccctgc cagatcctgg ccgacctgct gaccatctgg gagaagcgca
     2641 atcggctgga aggcctgcac atcgtgtgga tcggcgacgg caacaacgtc acccactcgc
     2701 tgatgatcgg cgcggccaag atgggcgtca acatgacggt gatcacgccg gagaacttcg
     2761 acgtgcagcc gaagtaccgc gacctggcgg aggccgccgc gcgggctacc ggcgcgcggc
     2821 tggagttcgg gcgcgacccc gccgccgtcg cgggcgccga tgtgatcttc accgacacct
     2881 gggccagctg gggccaggag gccgaggccg aggcgcgccg ccccatcttc cgcccctacc
     2941 aggtcaacac ggcgctgctg gagcgggccg ggccgcaggc cctggtcatg cactgcctgc
     3001 ccgcccaccg cggcgaagag atcaccgacg aagtgatcga cgggccgcgt
//
LOCUS       DASUYC010000822         5349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1114955, whole genome shotgun sequence.
ACCESSION   DASUYC010000822 DASUYC010000000
VERSION     DASUYC010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5349
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114955"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1391
                     /locus_tag="VF276_13445"
     CDS             <1..1391
                     /locus_tag="VF276_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607907.1"
                     /translation="GLIPYLIAQLIDPGNAILIVRIFVLLALIVSCWLLIAFAQRFLG
                     GPLTGLVAALISALYVATGSGYFTAPTILGEFWLGAKLEYFQLPFILACLYGVAWGSG
                     VQGERPRHANWGLFGAGLAWTVAVLIKPGAILVGPVCLLGLLLLWSQARPWRARLPEQ
                     GRAGAAFLAGAALPVALVYVPYFLRPATLDELRFNLLDLNSSYAVGPPIIVRVPALLL
                     GVPTVLLLLFLIAPVLVGRRLRGQPPTPAQRVLPMVLLAVPALFVGYLPGQALLHYLI
                     PIVPLMAMAVCGYLGLVFRDLARQGRPRLARALPFALALLYLAIQAPALLAYPGVAAR
                     DYYLDEDRTHFDLDGIVAYIKTHTAPTDPVWVYYDAPEINMLSGRPPATADPQADWLT
                     FLWADPWFDRTLAALRAEPPKLIIGVNHPHLYFARAVPITEVPKVRTLVASDYRCDTT
                     LLRGAIICTRLK"
     gene            1709..2977
                     /locus_tag="VF276_13450"
     CDS             1709..2977
                     /locus_tag="VF276_13450"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX6607908.1"
                     /translation="MVLLLVSSLLLGACDLGGSSPPASNTQPPSAQQPATTAPGGQGN
                     SSGGNGGSLGGGTGSSDNTPATATQNSGAVSQPGPSTAVFDKMAGGIPVNTTDPSGQP
                     VVQMVQKAGPAVVTVVNSLNPAATGGYGGEARGSGAIIDQAGYIITNNHVVEGQQSLV
                     VIFADGKKQQVQLVGTAPESDLAVLKASGTMPAVIPLGDSDKLLPGETVVAIGSALGE
                     FVNSVTVGVVSGLHRQLDEGNGVTISDLIQTDAAINHGNSGGPLLNLDGELIGINTLG
                     VTSAGQGDVAQGLGFAIPSNTVKSVAQRIMKGGTSGGQQASNRPYLGVRVQLVTPRLA
                     SYYNLVDTNGQLLNRGELVTEVLQGTPAEAAGLQAGDVIVGVDNQQIDSRTTLGAVLS
                     NHNAGDKVTMTIIRAGKQGTVQVTLGQAPG"
     gene            complement(3095..5113)
                     /locus_tag="VF276_13455"
     CDS             complement(3095..5113)
                     /locus_tag="VF276_13455"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HEX6607909.1"
                     /translation="MAASESETNGSAPSTPAPQASNPFLERLRRPGVIVADGAMGTML
                     YSKGVPANYCFDECNLTQPKLVLDIHQAYMEAGAEIIETNTYGANRFKLSTYDLGDKV
                     EQINFRGAKLAGNAREIVGRPVFIAGSVGPLGRPIQPLGRIAATEARDIFREQIEGLL
                     EGGVDLLILETFKDLREMLIALRAARESCDLPVVAQMSFEEDGRTQAGNTPAEVLRAL
                     EDAGADVVGANCSTGPVRMARVLADMAAVARAPLSALPNAGWPEVVAGRIVYVSGPTY
                     MAEQAQQMARMGVRILGGCCGTTPEHIRELARRLQGETAEEAATSAHTVAAAVASVHH
                     DPMLVTPYSAANGEAPGTSVVADEGEAHSVRETAQPGTFAWKLGREFVTTVELEPPRG
                     INPAKLLAGAQMLYDRGVSAVDITDSARGRASMSVMAVSNMVRQQVGAEVIIHFCTRD
                     RSLLGLQAELLGAHAMGQRIILALTGDPPNPVEKIASSGVFDIDSIGLIKLLRKMNAG
                     YDVAGNTIGGPTSFLIGAAFNPNARDLDLEIDRLYQKMEAGADFVMTQPFFDLEWLDK
                     TLDLVGDFKLPILMGILPLQSGRHAEFLHNEVPGITIPDWARARMHAAGANGRQEGIA
                     MAQELLSYAYKKVAGAYLMPSFGRYEVCAQVLEAAPEFRPRVVAEAAE"
BASE COUNT          796 a         1802 c         1802 g          949 t
ORIGIN      
        1 tcggcctgat cccctacctg atcgcgcaac tgatcgatcc cggcaacgct atcctgatcg
       61 tgcgcatctt cgtgctgctg gcgctgatcg tgagttgctg gctgctgatc gctttcgcac
      121 agcgctttct gggcgggccg ctgaccggcc tggtcgcggc gctcatctcg gcgctctatg
      181 tggcgaccgg ctccggctac ttcaccgcgc cgaccatcct gggcgaattc tggctgggcg
      241 ccaagctgga gtatttccag ttgccgttca tcctggcctg cctctacggg gtggcctggg
      301 gcagcggcgt gcagggcgag cgaccccgcc acgccaattg gggcctgttc ggcgcggggc
      361 tggcctggac cgtcgccgtg ctgatcaagc cgggcgccat cctggtcggc ccggtctgct
      421 tgctgggcct gttgctgctc tggtcgcagg cgaggccgtg gcgcgcgcgg ctaccggaac
      481 aggggcgggc gggggcggcg tttctggccg gggccgcgct gcccgtcgcc ctggtctatg
      541 tcccgtactt cctgcggccc gccacgctcg acgagctgcg ctttaacctg ctcgacctca
      601 attccagcta cgcggtgggg ccgccgatca tcgtgcgggt gccggcgctg ttgctcggcg
      661 tgccgaccgt gttgctgctg ctgttcctga tcgcgccggt gctcgtgggc cggcggttgc
      721 gcgggcagcc gcctacccct gcccagcgcg tgttgccgat ggtgctgctg gcggtgcccg
      781 cgctattcgt cggctacttg cccggccagg cgctgctgca ctacctgatc cccatcgtgc
      841 cgctgatggc catggcggtg tgcgggtacc tgggcctggt gttccgcgac ctggcccggc
      901 agggccgccc gcgcctggcg cgcgccctgc ctttcgccct ggccctgctc tacctggcga
      961 tccaggcccc ggcgctgctc gcctatccgg gcgtggccgc gcgggattac tacctggacg
     1021 aggaccgcac gcacttcgat ctcgacggca tcgtcgccta catcaagacg cacaccgccc
     1081 ccaccgaccc ggtctgggtc tactacgacg cgcccgagat caacatgctc agcgggcgcc
     1141 cgcccgccac cgccgatccc caggccgact ggctgacctt cctctgggcc gacccctggt
     1201 tcgaccgcac gctggccgcg ctgcgcgccg agccgcccaa gttgatcatc ggcgtcaacc
     1261 acccccatct ctacttcgcc cgcgccgtgc cgatcaccga ggtgcccaag gtgcgcacgc
     1321 tggtggcaag cgattaccgc tgcgacacca cgctgctgcg cggcgccatc atctgcacgc
     1381 ggcttaagta gcgccgggcc gcccgcagcc cttgcctctc gtcgataact cctgcgcggg
     1441 gcatgccttg cattggctgg ccttgcccac cgtcttgccc agatttttgg cacagacatt
     1501 gctcctctat ccagcaaaga tgttcccgca ctacaacgat gcctgtaaat aactctcttc
     1561 gctgtaggtt gcgatagccc cttgggcgcg gcacggactt gcagcggcac gacggccata
     1621 cggctgtgat atttcctgga ggtactcggt gatgggcacg tttcttcgct cccgcagcgc
     1681 tgggcggcgg tggcagcatt cggtaattgt ggttttactc ctggtcagta gtctgctgct
     1741 gggcgcgtgc gatctgggcg gcagctcccc cccggcgagc aatacgcagc cgccatccgc
     1801 acagcaaccg gccaccaccg cccccggcgg gcagggcaac tccagcggcg gcaacggcgg
     1861 cagcctcggc ggaggcacgg gtagcagcga caacacgccc gcgaccgcca cgcagaacag
     1921 cggcgctgtg agccagccgg ggccttccac cgccgtcttc gataagatgg cgggcggcat
     1981 cccggtgaac accaccgacc cctccggcca gcccgtggtg cagatggtgc agaaggccgg
     2041 ccccgcagtg gtcacggtgg tcaacagcct caaccccgcg gcgaccggcg gctatggtgg
     2101 cgaggcccgc ggtagcggcg ccatcatcga tcaggccggc tatatcatca ccaacaacca
     2161 tgttgttgaa gggcagcaat cgctagtcgt catcttcgcc gacggcaaga agcagcaggt
     2221 gcaactggtg ggcaccgcgc ccgagagcga cctggcggtg ctgaaagcca gcggcaccat
     2281 gccggccgtc attcccctgg gcgactcgga taagttgctg ccgggtgaaa cggtcgtcgc
     2341 catcggctcg gcgctgggcg agttcgtcaa cagcgtgacc gtcggcgtgg tcagcggcct
     2401 ccatcgccaa ctggatgaag gcaacggtgt gaccatctcc gatctgatcc agacggacgc
     2461 cgcaatcaac catggtaatt ccggcggccc gctgctgaac ctcgatggcg agttgatcgg
     2521 catcaacacg ctgggagtga ccagtgccgg gcagggcgac gtggcgcagg ggctgggctt
     2581 cgccattccg tcgaacaccg tcaagagtgt tgctcagcgg attatgaagg gcggcacatc
     2641 aggtgggcag caagcctcga accgcccgta tctcggcgtg cgcgtgcaac tcgtcactcc
     2701 gcggctggcg tcctactata acctggtgga caccaacggc cagttgctca atcggggcga
     2761 actggtgacc gaggtgttgc agggcacgcc cgccgaggcc gccgggctgc aggccggcga
     2821 cgtgatcgtc ggcgtggaca accagcagat cgacagccgg accacgcttg gcgctgtgct
     2881 gtcgaatcac aatgccggcg acaaggtgac aatgaccatc attcgcgcgg gcaagcaggg
     2941 cacggtccag gtgacgctgg gccaggcgcc cggctgatcc gcgtccggcc aaacaaaaac
     3001 ccgccccgac tcatcgccgg ggtgggtttt tgtttggtgc cgtatcggcg ggccggtttg
     3061 aaaccggccc ctaccgggcc gccggggcgg cgggctattc ggcggcttcg gcgaccacgc
     3121 gcgggcggaa ctcgggcgct gcttccagca cctgcgcgca gacctcgtag cggccaaagc
     3181 tcggcatcag gtaggcgccg gccactttct tgtaggcata gctcaacagt tcctgagcca
     3241 tggcgatgcc ttcctggcgg ccgttggccc cggcggcgtg catgcgggcg cgcgcccagt
     3301 ccgggatggt gatgcccggc acctcgttgt gcagaaactc ggcgtggcgc ccgctttgca
     3361 gcggcaaaat gcccatcagg atgggcagct tgaagtcgcc gacgaggtcg agtgtcttgt
     3421 cgagccattc caggtcgaag aagggctgag tcatcacgaa gtcggcaccg gcttccatct
     3481 tctggtagag ccgatcgatc tccaggtcga ggtcgcgcgc gttgggattg aacgcggcgc
     3541 cgatcaggaa gctggtcggg ccgccgatgg tgttgccggc cacgtcgtag cccgcgttca
     3601 tcttgcgcag cagcttgatc aacccgatgc tgtcgatgtc gaacacgcct gacgaggcga
     3661 tcttctcgac cgggttggga gggtcgccgg tgagggccag gatgatgcgc tgacccatgg
     3721 cgtgggcgcc cagcaactcg gcctgcaagc cgagcagcga gcggtcgcgg gtgcagaagt
     3781 ggatgatcac ctctgcgccc acctgctggc gcaccatatt gctgacggcc atgacgctca
     3841 tcgaggcccg gccgcgggcg ctgtcggtga tatccacggc gctgacgccg cggtcgtaga
     3901 gcatctgcgc cccggccagc agtttggccg ggttgatgcc gcgcggcggt tcgagttcga
     3961 cggtggtgac gaactcgcgg cccagcttcc aggcgaacgt gccgggttgc gccgtctcgc
     4021 gcacgctgtg cgcctcgccc tcatcggcca ccacgctcgt gccgggcgcc tcgccgttgg
     4081 ccgcgctgta cggcgtgacc agcatggggt cgtggtggac actcgctacg gcggcggcca
     4141 cggtatgggc gctcgtggcc gcttcctcgg cggtctcgcc ttgcaggcgg cgggccagtt
     4201 cgcggatatg ctccggtgtg gtgccgcagc agccgcccag gatgcgcacg cccatccggg
     4261 ccatctgctg ggcctgctcg gccatgtagg tgggcccgga gacatagacg atgcgcccgg
     4321 ccaccacttc gggccagccc gcgttgggta gcgcgctcag cggcgcgcgg gccacggcgg
     4381 ccatgtcggc cagcacccgc gccatgcgca ccggcccggt cgaacagttc gcgccgacta
     4441 cgtcggcccc ggcgtcttcc agggcgcgca gcacctcggc gggtgtgttg ccggcctgcg
     4501 tgcgcccgtc ttcctcgaaa ctcatctgcg ccaccaccgg caagtcgcac gactcgcggg
     4561 ccgcgcgcag ggcgatcagc atctcgcgca ggtctttgaa cgtctccagg ataagcaggt
     4621 ccacgccgcc ctccagcaag ccctcgatct gctcgcggaa gatgtcgcgc gcctcggtcg
     4681 cggcgatgcg ccccagcggc tgaatcgggc ggcccagcgg gcccaccgac ccggcgatga
     4741 acacgggccg gcccacgatc tcgcgggcat tgcccgccag cttcgcgccg cggaagttga
     4801 tctgctcgac cttgtcgccc aggtcgtagg tgctcagctt gaagcggttg gcgccatagg
     4861 tgttggtctc gatgatctcg gccccggctt ccatataggc ttggtggatg tcgagcacca
     4921 gcttgggctg cgtgagattg cactcgtcga agcaatagtt ggccgggacg cccttgctgt
     4981 agagcatggt gcccatggcg ccgtcggcca cgatcacgcc gggccggcgc aaccgttcga
     5041 ggaaggggtt gctcgcctgg ggcgcgggcg tggatggggc cgacccgttc gtttcggatt
     5101 cagatgctgc catagagttc tcctgtataa cctgccgaaa gtgtgcaacg gtaattatac
     5161 cggagggacc ccagcacggc aaccggcccg gcccggttat ccactgtcac ggtggataag
     5221 gtggtgcata aagtggtgga taaaggggct tgacgccgcc gggcggcggt gctatgatga
     5281 gggtgcttca gtaaattgtt cttcggaact atatattggg gtagggaagc cggaggatcc
     5341 gcaaggatt
//
LOCUS       DASUYC010000823         2972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1746691, whole genome shotgun sequence.
ACCESSION   DASUYC010000823 DASUYC010000000
VERSION     DASUYC010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1746691"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /locus_tag="VF276_13460"
     CDS             <1..1091
                     /locus_tag="VF276_13460"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEX6607910.1"
                     /translation="LHLRAQGLYRPPSAATPDVAQIVHEAAGLQAQDAAAGMRAVGVR
                     GPGLVVGDVEHARVAARTVVRTWAMRGTLHLVAATDLRGLLALFGPVFLRGDARRRTQ
                     LGLDDATCARAMRLIRDAITAEGPRTRADLAQRLAADGIPTAGQAIVHLLYYAALSGL
                     ICQGPDRDGEPAFVLLDEWVAPAPAPARPQALADLARRYLAAYGPAGPDDLAAWSGLP
                     KPDLRLAWAGIADEVLAVEADGQPAWLLKTRTEWLDDPLPAQPVVRLLPAFDTYLLGY
                     RDRRLSVAPDHARHIHPGGGILHPALLVDGRAQGVWRSGRKRDRLEVVVAPFADLSAP
                     VQAGLEAAAADLGRFLGLPADLRVARRP"
     gene            complement(1181..2722)
                     /gene="lysS"
                     /locus_tag="VF276_13465"
     CDS             complement(1181..2722)
                     /gene="lysS"
                     /locus_tag="VF276_13465"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX6607911.1"
                     /translation="MDDDSTAYPGLEFGDQEQQRLAKLRQLREQGLDPYPPRFRRTHT
                     PADTLAAFAEWEAAHPAAADGTRDAEPLRVRTAGRVMGRRIMGKASFAHIASGGTRLQ
                     LFFRADDLPTPPNDYDAFKKLVDMGDHIGVEGFMFRTKTGEPSLHVESFELLSKSLTP
                     LPEKWHGLQDQELRYRQRYLDLIANDDVRQVFERRSALVSAMRRYLDGHGFLEVETPV
                     LQPLYGGATARPFTTHHNTLDQQLYLRIADELYLKRLIVGGFDKVYEIGHDFRNEGID
                     VRHNPEFTMMECYEAYGDYGTMMELVEGLIVYMAQEVLGTLQIRHRGRDIDLTPPWRR
                     ITMREALKQAGGIDFADYYDDRDGFYERVRQIQGVEVNPKMTWAQILDEVVGTVVEPA
                     LVQPTFLMDYPAAISPLAKRSRQDPHLVERFEAFISGFEMGNAFTELNDPLDQYQRFA
                     ASAADRRAGDEEAHQMDLDFINALMVGMPPTGGLGMGVDRLCMVLLDQPSIRDVILFP
                     HMRPR"
     gene            complement(2802..>2972)
                     /locus_tag="VF276_13470"
     CDS             complement(2802..>2972)
                     /locus_tag="VF276_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HEX6607912.1"
                     /translation="DEIHWTIVGSAEANTRQGKISNESLVGAALMGHGPGEVVSVQAP
                     GGLQQFTILKIE"
BASE COUNT          382 a         1000 c         1105 g          485 t
ORIGIN      
        1 tgctgcatct gcgggcgcag ggactctatc gcccaccatc cgccgccacc ccggatgtgg
       61 cgcagatcgt ccatgaggcg gcgggtctcc aggcccagga tgccgccgcg gggatgcgcg
      121 cggtgggggt ccgcgggccg ggcctggtgg tcggcgatgt cgagcatgcg cgggtcgcgg
      181 cgcgcacggt ggtgcgcacc tgggccatgc gcggcaccct gcacctggtg gcggcgaccg
      241 atctgcgcgg gctgctggcg ctgttcggcc ccgtctttct gcgcggtgac gcgcgacggc
      301 gcacgcaact gggcctggac gacgcgacct gtgcgcgggc tatgcgcctg atccgcgacg
      361 cgatcaccgc cgaagggccg cgcactcgtg ccgacctggc ccagcgcctc gcggcggacg
      421 gcatccccac ggcggggcag gccatcgtcc acctgctcta ctacgccgcg ctgagcggtc
      481 tcatctgcca ggggccggac cgggacggcg agccggcctt tgtgctgctg gacgagtggg
      541 tcgccccggc ccccgcgccg gcacgcccgc aggcgctggc ggacctggcc cggcgctatc
      601 tggcggccta tggcccggcg ggacccgacg acctggcggc ctggtcgggc ttgcccaagc
      661 ccgacctgcg cctggcctgg gcgggcatcg cggacgaggt gctggcggtg gaggcggacg
      721 ggcagccggc ctggctgctc aagacgcgca ccgagtggct ggacgatccg ctgcccgccc
      781 agcccgttgt ccgcctgctg cccgcctttg atacctacct gctgggctac cgcgaccggc
      841 gcctgagcgt ggcgcccgac cacgcccggc atattcaccc cggcggcggc atcctgcatc
      901 ccgcgctgct ggtcgatggc cgggcgcagg gagtctggcg cagcggccgc aagcgtgacc
      961 ggctggaggt cgtcgtggcg ccctttgccg acctgtccgc cccggtgcag gcgggcctgg
     1021 aagccgcagc ggcggacctg ggccgcttcc tgggcctgcc cgccgatctg cgcgtggccc
     1081 ggcgtccctg atcggcgggc gcggccgtct ataccgtcga aagcagcagg ggcgcccaat
     1141 ggcgcccctg tgcggcgatt cggttgtggt gccggcacgg ttagcgcggg cgcatgtgcg
     1201 ggaacaggat cacgtcgcgg atcgagggct ggtcgagcag gaccatgcac agccggtcca
     1261 cacccatgcc cagcccgccg gtcggcggca tgccgaccat gagcgcgttg atgaagtcga
     1321 ggtccatctg gtgggcctcc tcgtcgcccg cgcggcggtc ggcggcgctc gcggcgaagc
     1381 gctggtactg gtccagcggg tcgttgagct cggtgaaggc gttgcccatc tcgaagcccg
     1441 agatgaacgc ctcgaagcgc tcgaccaggt gcgggtcctg ccggctgcgc ttggcgagcg
     1501 gcgagatggc cgccgggtag tccatcagga acgtcggctg caccagcgcc ggctcgacga
     1561 ccgtgcccac cacctcgtcg aggatctgcg cccaggtcat cttcgggttc acttcgacgc
     1621 cctggatctg gcgcacccgc tcatagaagc cgtcgcggtc gtcgtagtag tcggcgaagt
     1681 cgatgccgcc ggcctgcttc agcgcctcgc gcatggtgat gcggcgccag ggcggcgtca
     1741 ggtcgatgtc gcgcccgcga tggcggatct gcagcgtgcc cagcacctct tgcgccatgt
     1801 agacgatgag gccttccacg agttccatca tggtgccgta gtcgccgtag gcttcgtagc
     1861 actccatcat ggtgaactcg gggttgtggc gcacgtcgat gccttcgttg cggaagtcgt
     1921 gcccgatctc gtagaccttg tcgaacccgc cgacgatcag ccgcttcagg tagagttcgt
     1981 cggcgatacg caggtagagt tgctggtcga gcgtgttgtg gtgggtggtg aaggggcgcg
     2041 cggtggcccc gccgtagagc ggctgcaaca ccggcgtctc gacttccagg aagccgtggc
     2101 cgtccaggta gcggcgcatg gcgctgacca gagccgagcg gcgctcgaac acctggcgca
     2161 cgtcgtcatt ggcgatcagg tcgaggtagc gctggcggta gcgcaactcc tggtcctgca
     2221 agccgtgcca cttttcgggc agcggtgtga ggctcttgct gagcagttcg aagctctcga
     2281 cgtgcaggct cggctcgccc gtcttggtgc ggaacatgaa gccctcgacg ccgatgtgat
     2341 cgcccatgtc gaccagcttc ttgaaggcgt cgtagtcgtt gggcggggtg ggcaggtcgt
     2401 cggcgcggaa aaagagttgc aggcgcgtgc cgccggacgc gatatgggca aacgaggcct
     2461 tgcccatgat gcgccggccc atgacgcgcc ccgccgtgcg cacgcgcagc ggttcggcgt
     2521 cgcgcgtgcc gtcggcggcg gcggggtggg ccgcttccca ctcggcaaag gcggcgaggg
     2581 tgtcggcggg ggtgtgggtg cggcggaagc gcgggggata gggatcgagg ccctgttcgc
     2641 gcaactggcg cagcttggcg aggcgctgct gctcctggtc gccaaattcg agtccgggat
     2701 aggcggtgga gtcgtcgtcc ataaatgctt gtccttgacg gcgaccccgc gccccggccc
     2761 ggcggccggc acgcgacgcc cgcccgtgat gccgtggcgg cttactcgat cttcagaatg
     2821 gtgaactgct gcagaccgcc gggcgcctgc acgctgacga cctcgccggg gccgtggccc
     2881 atcagggccg cgccgaccag cgactcgttg gagatcttgc cctggcgggt gttggcttcg
     2941 gccgagccga cgatggtcca gtggatttcg tc
//
LOCUS       DASUYC010000824         2449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_129418,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000824 DASUYC010000000
VERSION     DASUYC010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129418"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1343
                     /locus_tag="VF276_13475"
     CDS             <1..1343
                     /locus_tag="VF276_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011321359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6607913.1"
                     /translation="EARPAPAAKRAARGRTAGAARHGASTATSMTGDGPGAAATFEES
                     EVPDDRHNIGEGDRVLLIVEDDPHFARTLLAMAREKGFKGLVALYGETALMLAREFQP
                     DAITLDIALPLMDGWTVLDRLKHDPVTRHIPVHIITGSEEPARGLRQGAIAYLQKPVT
                     EPRLSEALGNIKGFVERPVKNLLVVEDDDVQRQSIVDLIGNRDVHTTAVGTGADALAA
                     LQSTQFDCLVLDLVLPDMTGFALIEKIKKDLGLTALPIVIYTGKDLTRQEETQLRRLS
                     DTIIVKDVNSPERLLDETALFLHRVEANLPEAKRQILAELHQKDPALTGKKLLIVDDD
                     IRNIFALTSLLERYDMEVLYAENGKDGIKLLQTTPGIDVVLMDVMMPEMDGYATMQAI
                     RKMKKYRELPIIALTAKAMKGDRERCIEAGASDYVPKPVDPAQLLSLLRVWLYR"
     gene            1351..2187
                     /locus_tag="VF276_13480"
     CDS             1351..2187
                     /locus_tag="VF276_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEX6607914.1"
                     /translation="MTTTRAPEDLEGLEIQLLLEGVYQHYGYDYRQYAPASLRRRIAG
                     AVQAEGLQTVSGLQERVLHDPACMERLLLALSVNVSAMFRDPSFYLAFRRRVVPLLRT
                     YPSIDIWHAGCSSGEEVYSMAILLEEEGLAGRCRLYATDVNEAVLRKARAGIFPLEFL
                     KDWTNNYLEAGGTRAFSDYYTAGYGSAIFRAGLRDNIVFAQHNLVTDGAFQQFHVIWC
                     RNVTIYFTRPLQQRVHGLLYDSLIRLGVLGLGSQESIDGTPHAAAYEPLAGREKLYRK
                     VR"
     gene            2187..>2449
                     /locus_tag="VF276_13485"
     CDS             2187..>2449
                     /locus_tag="VF276_13485"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEX6607915.1"
                     /translation="MAYALVVVGASLGGIRALQTVLEGLPAAFAAPVVVVQHCGPGHD
                     SVRRAQLREFSALPVATAEDKMDLLPGHVYVAPADYHLLVEAG"
BASE COUNT          428 a          821 c          822 g          378 t
ORIGIN      
        1 ccgaggcccg cccggccccg gcggcgaaac gggcggcgcg gggacgcacg gcgggggcgg
       61 cacggcacgg cgcttccacc gctacgtcca tgaccggaga cggaccgggc gcggcggcga
      121 ccttcgagga gagcgaggtg cccgacgacc gccataacat cggcgagggc gaccgggtgc
      181 tgctgatcgt ggaggacgac ccgcacttcg cgcgcaccct cttggcgatg gcccgcgaga
      241 agggcttcaa gggcctcgtt gcgctctacg gcgagacggc gctgatgttg gcgcgcgagt
      301 tccagcccga cgcgatcacg ctcgacatcg ccctaccgct catggacggt tggacggtgc
      361 tcgaccgctt gaagcacgat ccggtgacac gacatatccc tgtgcatatc atcacaggct
      421 ccgaggaacc ggcgcgcgga ctgcgccagg gcgccatcgc ctatctgcag aagcccgtca
      481 ccgagccgcg gctcagcgag gcgctgggca acatcaaggg ctttgtcgag cgcccggtca
      541 agaacctgct ggtggtggag gacgacgacg tgcagcgcca gagcatcgtc gatctcatcg
      601 gcaaccgcga cgtgcatacc accgccgtgg gcaccggcgc ggacgcgctg gcggcgctgc
      661 aaagcacgca gttcgattgc ctggtgctcg acctggtgct gcccgacatg accggcttcg
      721 cgctgatcga aaagatcaag aaggacctcg gcctcaccgc cctgcccatc gtgatctaca
      781 ccggcaaaga cctgacccgc caggaggaga cgcaactgcg ccgcctctcg gacacgatca
      841 tcgtcaagga cgtgaactcg ccggagcgcc tgctggacga gacggcgctg ttcctccacc
      901 gggtggaggc caacctgccc gaagccaagc gccagatcct ggccgaactc caccagaagg
      961 accccgcgct gaccggcaag aagctgctca tcgtggacga cgacatccgc aatatcttcg
     1021 cgctgaccag cctgctggag cgctacgaca tggaggtgct ctacgccgaa aacggcaagg
     1081 acggcatcaa gctgctgcaa accacgcccg gcatcgacgt ggtgctgatg gacgtgatga
     1141 tgcccgagat ggacggttac gcgacgatgc aggccatccg caagatgaag aaataccgcg
     1201 aactgcccat tatcgcgctc acggcgaagg cgatgaaggg cgaccgcgaa cgctgcatcg
     1261 aagcgggcgc ctcggactac gtgcccaagc cggtcgaccc ggcgcagttg ctgtcgctgc
     1321 tgcgcgtctg gctgtaccgc taggcccgcc atgaccacga cccgcgcgcc ggaggacctg
     1381 gagggcctgg agatccagtt gctgctggag ggcgtctacc agcattacgg ctacgactac
     1441 cgccagtatg ccccggcctc gctgcggcgc cgcatcgccg gcgccgtgca ggccgagggg
     1501 ctgcaaacgg tgtcgggctt gcaggaacgc gtgctgcacg atcccgcgtg catggagcgt
     1561 ctgctgctgg cgctgtcggt caacgtcagc gccatgttcc gcgaccccag cttctacctg
     1621 gctttccggc ggcgggtggt gccgctgctg cgcacctacc cgtccatcga catctggcac
     1681 gccgggtgtt cgagcgggga agaggtctac tcgatggcga tcctgctgga agaagaaggg
     1741 ctggccgggc gctgccgcct ctacgccacc gatgtcaacg aggcggtgct gcgcaaggcc
     1801 cgcgccggga tcttcccgct ggaattcctg aaggactgga ccaacaacta cctggaggcg
     1861 ggcggcacgc gcgccttctc cgactactat accgcgggct acggcagcgc gattttccgc
     1921 gccggcctgc gcgacaatat cgtctttgcc cagcacaacc tggttacgga cggcgctttc
     1981 cagcagttcc acgtgatctg gtgccgcaac gtgaccatct actttacgcg ccccttgcag
     2041 cagcgcgttc acggcctgct ctacgacagc ctgatccgcc tgggcgtgct ggggctgggc
     2101 agccaggagt caatcgacgg cacgccccat gcggcagcct atgaaccgct ggccggacgg
     2161 gaaaagctct accggaaggt acgctgatgg cctatgccct ggtcgtcgtg ggcgcgtcgc
     2221 tgggcggcat aagggccctg caaacggtgc tggagggctt gcccgccgcg tttgccgcgc
     2281 cggtggtcgt ggtgcagcac tgcggccccg gccatgatag cgtgcgccgg gcgcagttgc
     2341 gcgagttcag tgcgctgccc gtcgccacgg cggaggataa gatggacctg ttgccgggcc
     2401 acgtctacgt ggcgcccgcc gactatcatc tgctggtgga ggccgggag
//
LOCUS       DASUYC010000825         3878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1284004, whole genome shotgun sequence.
ACCESSION   DASUYC010000825 DASUYC010000000
VERSION     DASUYC010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284004"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VF276_13490"
     CDS             complement(<1..243)
                     /locus_tag="VF276_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014037717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HEX6607916.1"
                     /translation="MRTVTATRYVTPLREGGSLPAIVEADDDGTYVLKFRGAGQGPKA
                     MIAELVAGEMARVAGLPVPEIVFIELDADLARTEPDP"
     gene            complement(343..909)
                     /locus_tag="VF276_13495"
     CDS             complement(343..909)
                     /locus_tag="VF276_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607917.1"
                     /translation="MDSPSILATWYYTEEDWAAYDAREWARDRRQAGTTFRLVCALAV
                     VFGLIIAVFSYPSGMACFGLAPLVLWGVPFGWIYRTEYKRAAARHQARLAGPRQIRIT
                     AQGLYVAGAFVPLAPRTPLFAFAGFLSESPLQVHWIAVTHGTRLELCLRGYRERRRDE
                     LCVPVPHGCEAEAGPVAERLRRELLRSR"
     gene            1031..1279
                     /locus_tag="VF276_13500"
     CDS             1031..1279
                     /locus_tag="VF276_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006433289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HEX6607918.1"
                     /translation="MWEYLSLYAERVDTQETFRVKNYPEPGSRVDEQELMNRLGAEGW
                     ELVAVVAAYGGGGTKINHQLYFKRPARAWGEGTQRLDR"
     gene            complement(1451..3835)
                     /locus_tag="VF276_13505"
     CDS             complement(1451..3835)
                     /locus_tag="VF276_13505"
                     /EC_number="3.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004339 - glucan 1,4-alpha-glucosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009251 - glucan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan 1,4-alpha-glucosidase"
                     /protein_id="HEX6607919.1"
                     /translation="MDNATHDAPGCPGIPARWTSSAKTGVGTALDAGSRVWFTLSHGI
                     FNEIYYPRVDQACTRDLGLIVTDGEAYFSEEKRHTRSTVTTAAPGVPAYHLTNVAEDG
                     RYRIEKEIVADPSRDAVLQHTRFVPLQGHLADYRLYALLAPHLGNQGAGNTAWVGDHK
                     GVPMLFAERGGLALALVCSTPWLRGSAGFVGVSDGWQDLQQHRQLTWLYDRAENGNVA
                     LTGEIDLVACGGVFTLVLAFGVNPAEAGHRARSSLKQDFATTARHYVEEWQHWQRTLH
                     PVPDPGKGPRDLYAVSAAVLRMHEDKNFAGAFIASLSIPWGFAKGDHDMGGYHLVWPR
                     DLVEAAGGLLAAGSLPEAARVLQYFQATQEADGHWAQNMWIDSTPYWNGIQLDETALP
                     ILFLHLAQREGALAGSDWRALWPMVRRAARYIVRNGPVTQEDRWEEDAGYTAFTLGAE
                     IAALLVAADLAESCGEPDLAPYLRETADAWNASIERWTYVSGTDLAQQVGVAGYYLRI
                     APPGHTGTVIIKNRPANESQEPAGAIVSPDALALVRFGLRAPDDPRITDTVKVIDALL
                     KVDTPRGPAWHRYNDDGYGEHADGAPFDGTGIGRIWPLLTGERAHYELAAGRRAAAVR
                     LLAALESFANEGGLLPEQIWDSPDIPARELYCGRPSGSAMPLVWAHAEYVKLRRSLAE
                     GRVFDLPEAAARRYLPTPPDSPYALWRFNNKARTMPAGKILRVETQAPAVVRWSGDGW
                     QTVQDTPTRDTGLGMYVADLPTTGLPAGATVTFTFRWPAANRWEGRNFEVQVRG"
BASE COUNT          632 a         1366 c         1297 g          583 t
ORIGIN      
        1 cggatcgggc tcggtgcgtg cgagatccgc atccagctcg atgaacacga tttcgggcac
       61 cggcaggccc gcgacgcgcg ccatctcgcc ggccacgagc tccgcgatca tcgccttggg
      121 cccctgcccg gcgccgcgaa acttgagcac ataggtgccg tcgtcgtcgg cctcgacaat
      181 cgcgggcagc gagccgccct cgcgcaacgg ggtcacatag cgggtcgccg tcacagtacg
      241 cagcatgtag tagtcgtatc cttgcccggc cgggcgtggc gaatcgcgcg cgccgggtgg
      301 cccgcacgca ggcccattat agccggaaag gctgcggtgc ggctagcgcg accgcaataa
      361 ctcgcggcgc agccgttcgg ctactggtcc ggcttcggcc tcgcacccat gcggaaccgg
      421 gacgcaaagc tcatcgcgcc gccgctcccg gtagccccgc agacataatt ccagccgcgt
      481 accgtgagtc acggcaatcc aatgcacctg caacggcgac tcggacagaa atccagcgaa
      541 agcgaacagc ggcgtgcgcg gggccagcgg aacaaaggcc ccggcaacat atagcccctg
      601 cgcggtgatg cgaatctggc gaggcccagc gagacgcgcc tgatggcgcg ccgccgcacg
      661 cttgtactcg gtgcgataga tccacccgaa cggcaccccc cacaggacga ggggcgccaa
      721 accaaaacac gccatccccg acggataaga aaagacggcg ataatcagtc cgaaaacgac
      781 cgccagggcg cacactaacc taaacgtggt gccggcctgc cgccgatccc gcgcccactc
      841 acgggcatca tacgccgccc agtcttcctc tgtgtagtac caggttgcta aaatcgacgg
      901 agagtccatg ccccattata cccaggcacc caatcacctc gcgatctggg atcgctgcaa
      961 ctcaacgcgc ccgcttgcgt aagcatgggt agatgtgatg tcacgcatgt acagaggaag
     1021 gagcagaccg atgtgggaat atctgagcct ctacgccgag cgcgtagaca cgcaggagac
     1081 gttccgggtg aagaactacc cggaaccggg cagccgcgtg gacgagcagg aactcatgaa
     1141 ccgcctgggc gccgagggct gggaactggt cgccgtggta gccgcctacg gcggcggcgg
     1201 cacgaagatc aatcaccagc tctacttcaa gcgcccggcc cgtgcgtggg gcgaaggcac
     1261 gcagcggctc gaccgctagc cccagaccag tgctgagtgc tgagtgctga gtgctgagtt
     1321 gtcctaccag tccgtcttgt cactctgagc cgagcgaaga gtctcatcgc gcgtgtggcg
     1381 agacggagat tcttcgctcc gctcagaatg acagttctcg tgtgacaatg tattcagcac
     1441 tcagcactga tcagccccgc acctgcacct cgaaattgcg cccttcccag cggttcgccg
     1501 cgggccagcg gaaggtgaag gtgacggtgg ccccggcggg caggccggtg gtgggcaggt
     1561 cggccacgta catgcccagg cccgtgtcgc gggtgggggt gtcttgcacg gtctgccagc
     1621 cgtcgccgct ccagcggacg accgcgggcg cctgtgtttc cacgcgcagg atcttgcccg
     1681 cgggcatggt gcgcgctttg ttgttgaagc gccacagcgc atacggcgag tccggcggcg
     1741 tgggcagata gcgtcgcgcc gccgcttcgg gcagatcgaa cacccggccc tcggccagtg
     1801 agcggcgcag cttcacatac tcggcgtgcg cccagaccag cggcatggcc gagcccgagg
     1861 gccgcccgca atagagttcg cgcgccggga tgtcgggcga gtcccagatc tgctcgggca
     1921 gcaagccgcc ctcgttggcg aacgactcca gggccgccag cagccgcacc gccgcggccc
     1981 gccgccctgc cgccagttcg tagtgcgcgc gctcgccggt cagcagcggc cagatgcgcc
     2041 cgatgcccgt gccgtcgaag ggcgcgccgt ccgcatgctc gccgtagccg tcgtcgttgt
     2101 agcggtgcca ggccgggccg cggggcgtgt cgactttcag cagcgcgtcg atcaccttca
     2161 cggtgtcggt gatgcgcggg tcgtcgggcg cgcgcaggcc gaagcgcacc agcgccagcg
     2221 cgtcggggct gacgatagcg cccgccggtt cctgggactc gtttgccggg cggttcttga
     2281 tgatcaccgt gccggtgtgc cccggcgggg cgatgcgcag gtaatagccc gccacgccca
     2341 cctgctgggc caggtcggtg ccgctcacat aggtccagcg ctcgatgctc gcgttccagg
     2401 catcggcggt ctcgcgcagg tagggcgcca ggtccggttc gccgcaggac tcggccaggt
     2461 cggcggccac cagcagggcc gcgatctcgg cgccaagcgt gaaggccgtg tagcccgcgt
     2521 cctcctccca gcggtcctcc tgcgtcaccg gcccgttgcg cacgatgtag cgcgccgccc
     2581 ggcgcaccat gggccagagc gcgcgccagt ccgacccggc cagcgccccc tcgcgctgcg
     2641 ccaggtgcag gaacaggatc ggcagggccg tttcgtccag ttggatgccg ttccagtagg
     2701 gcgtgctgtc gatccacata ttctgcgccc agtggccgtc ggcctcctgg gtggcctgga
     2761 agtattgcag cacccgcgcc gcctccggca agctgcccgc cgccagcagg ccgcccgccg
     2821 cttccaccag atcgcgcggc caaaccaggt ggtagccgcc catgtcgtgg tcgcccttgg
     2881 cgaagcccca ggggatgctc aggctggcga taaacgcccc ggcgaagttc ttgtcctcgt
     2941 gcatgcgcag caccgccgcg ctcacggcat agagatcgcg cggccccttg ccggggtcgg
     3001 gcacggggtg cagcgtgcgc tgccagtgct gccattcctc aacgtagtgc cgggccgtgg
     3061 tggcgaagtc ctgcttgagg ctggaccgcg cccggtggcc cgcctccgcc gggttcacgc
     3121 cgaaggccag caccagggtg aacacgccgc cgcaggccac caggtcgatt tcgccggtga
     3181 gggccacgtt gccgttctcg gcccggtcat acagccacgt cagttgccgg tgctgttgca
     3241 gatcctgcca gccgtcggac acgcccacga agcccgccga cccgcgcagc cagggcgtcg
     3301 agcagaccag cgccagcgcc agcccgcccc gctcggcaaa cagcatcggt acgcccttgt
     3361 gatcgcccac ccaggccgta ttgcccgcgc cctggttgcc caggtggggc gccagcagcg
     3421 catagagccg gtaatcggcc aggtggccct gcaacggcac aaagcgcgtg tgctgcaaca
     3481 ccgcgtcgcg cgagggatcg gccacgatct ccttctcgat gcggtagcgc ccgtcctcgg
     3541 cgacattggt caggtgataa gcgggcacgc cgggcgccgc cgtggtcacc gtggaccggg
     3601 tgtggcgctt ctcctccgag aaataggcct cgccgtcggt cacgatcagc cccaggtcgc
     3661 gggtgcaggc ctggtccacg cgcggatagt agatctcatt gaagatgccg tggctcaggg
     3721 tgaaccagac acggctgccg gcgtccagcg ccgtgcccac gccggtcttg gcgctcgaag
     3781 tccagcgcgc cgggatgccc gggcatcccg gcgcgtcatg tgtcgcatta tccatcgctg
     3841 tcagggtcca acctcgcgcc gccgggagcg ggccgccc
//
LOCUS       DASUYC010000826         3516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1747724, whole genome shotgun sequence.
ACCESSION   DASUYC010000826 DASUYC010000000
VERSION     DASUYC010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1747724"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1780
                     /locus_tag="VF276_13510"
     CDS             <1..1780
                     /locus_tag="VF276_13510"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEX6607920.1"
                     /translation="PPCVGQWGTVAVPGAAPGSRINGLATIAQNDVWAAGYTTNAQGV
                     EQTLVLHWDGSVWNVVPSPNHGSGNNRLNTVTARAANDVWAVGQAAGGGGTTTLILHW
                     DGSAWTETASPSPGVTNNQLSGVVSLAADNAWAVGGADMNTLVLQWNGTNWTQVASPS
                     PGGIYGASFLNSVSALAANDIWAAGAYQAMGYGETLVLHWDGSSWTQVASPSPGMQYD
                     DVLKSMVMLSAGDGWAVGSYTFNNSRRTLTLHWDGTSWTQVPSPAGGSGDNFLSSSAA
                     GGPTDVWAVGNYASGAAQQTMILHWNGSGWALVPSGNVGTGDNRLNAVAAGPAGEVWA
                     AGSYGAGTTARPLMEHYPADCASPTPTATPPAATATATRTTVPAATATATPAATACPV
                     QFADVPSTNPFYSFIRCLACRSIVGGYADNTFRPYNGVTRGQTAKFVANAAGYSDAIP
                     LTRQTFTDVPNSDPFWLYIERAYSHGVISGYSNGDGSLSFRPSNPVTRGQMAKFVANA
                     ANLTDDVTGRQTFSDVPGADPFWLFIERAALHGIISGYTCGTAPAGACDNLHRPYFLA
                     GNAVSRGQAAKFISNSFFPNCQTPRR"
     gene            2229..>3516
                     /locus_tag="VF276_13515"
     CDS             2229..>3516
                     /locus_tag="VF276_13515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX6607921.1"
                     /translation="MAARASTIREPAGVTAAERAGQMGPWIVALLVAFTLVRGVIWAY
                     AIPPLNAPDEPSHLIAVSQIRALRGLPVAILIDPNTLSENSSPRPPEVLAYLAQPQFH
                     YTRFRALSYESTQPPLYHLLCAVLTRPLPADPETLLYGCRLVSALLGALTVFAVARAA
                     ATLAPRQPAFALGAPLALTLWPQFGFQTATLNNDIASTLAGAALAWAWAAAMRRPGDR
                     RWTAGLGALTGLGMLCKLTVAATLPGTGLILLGRARAGGDTLRRFVGEAVLAGAVAAV
                     PVLPWVWRNLAVYGEPTGAAQMLSVIHSIYTTRLGLPAETRFIIPPLGDFVWYSFSSW
                     WGVFGWRSVFLPGPLYVVAAGLTLLGAGGATTWLVRRVRTRQPPAPAQGWPLAACAVT
                     IAAALANYILYTVTTDASLQGRYTFVALAAVCLVGVS"
BASE COUNT          531 a         1218 c         1261 g          506 t
ORIGIN      
        1 gccgccctgc gtgggccagt ggggcacggt ggccgtgccg ggagcggccc ccggcagccg
       61 gatcaacggc ctggcgacca tcgcgcaaaa tgatgtgtgg gccgcgggct acacgaccaa
      121 tgcccagggg gtcgaacaaa cgctggtgtt gcattgggac ggcagcgtat ggaacgtcgt
      181 gcccagcccc aaccacggga gcggcaacaa ccggctcaac acggtgacgg cccgcgcggc
      241 gaatgatgtc tgggccgtcg gtcaggccgc aggcggcggc ggtacgacca ccctgatcct
      301 acactgggac ggcagcgcct ggacggagac ggcaagcccc agccctggag tcaccaataa
      361 tcagctcagc ggagtggtga gcctggccgc ggacaatgcc tgggccgtgg gcggcgcgga
      421 catgaacacc ctggtcctgc agtggaacgg caccaattgg acgcaggttg ccagtcccag
      481 tccggggggc atctacggcg ccagtttcct caacagcgtc agcgccctgg cggcgaacga
      541 catctgggcg gccggcgcgt accaggcgat gggctacggc gaaaccctgg tcctgcactg
      601 ggacggcagc agttggacgc aggtcgccag ccccagcccc ggcatgcagt acgacgatgt
      661 gctcaaaagc atggtgatgc tctcggccgg cgacggatgg gccgtggggt cgtacacctt
      721 caacaatagc cggcgcacgc tgaccctgca ctgggacggg accagctgga cccaggtgcc
      781 cagtccggcg ggcgggtcgg gcgataactt cctgtccagc agcgccgccg gagggccgac
      841 tgatgtgtgg gccgtgggca actatgccag cggtgccgcg caacagacga tgatcctgca
      901 ttggaacggc agcggctggg ccctggtgcc gagcggcaac gtgggcacag gcgacaatcg
      961 gctcaatgcc gtggccgccg ggccggccgg cgaggtctgg gcggcgggat cgtatggtgc
     1021 gggcaccacg gcccggcccc tgatggagca ttatccggcg gactgcgcgt cgcccacgcc
     1081 caccgccaca ccaccggccg caaccgcgac ggccacccgc accaccgtgc cggccgcaac
     1141 cgcaactgcg acgcccgctg ccaccgcatg cccggtgcag tttgcggacg tgccgagtac
     1201 caaccccttc tacagcttca tccgctgcct ggcctgccgg agcatcgtcg ggggttacgc
     1261 ggacaacacc ttccggccgt ataatggcgt cacccgcggg caaaccgcca agttcgtcgc
     1321 caatgcggcg gggtacagcg acgcgatccc cctcacgcgc cagacgttca ccgatgtgcc
     1381 gaacagcgat ccgttctggt tgtacatcga gcgggcctac agccatgggg tgatcagcgg
     1441 ctacagcaac ggcgacggct cgctcagttt ccgccccagc aaccccgtca cgcgcggcca
     1501 gatggcgaaa ttcgttgcga acgcggccaa tctgacagat gacgtcaccg ggcggcagac
     1561 cttcagcgat gtgcccggcg ccgatccgtt ctggctgttc atcgagcggg cggcgctgca
     1621 cgggatcatc agcggctata cctgtggcac ggcgcctgcc ggggcctgcg acaatctgca
     1681 ccggccctat ttcctggcgg ggaacgcggt gagccgcggg caagcggcga agtttatcag
     1741 caacagcttc ttcccgaact gccagacgcc ccggcgctag tgcaggctcc agccacctgt
     1801 tggggcagcc gcctctaaag cccgacgagt acgggcgcga taaatcgcgc cgctacacac
     1861 aacaggtggt tggagcctgc actaggcagc cggcacggtg attttgcggt gtaggggccg
     1921 gtatggaacc gacctctaca ccggcaatcg ccgggcgccg caccagggcc ggcacaccgg
     1981 tcggccccta caagctgcca acctctcccc cggctggggt acatcccccg gcgcggagag
     2041 ggcagtcact ttcaattgcg aaagcgattc cccccttccc gcggcgggaa gggggccggg
     2101 gggtcaggtt gcccgccgca cacgccgcgc cgccgcccgg tttttggtat actgagacgg
     2161 cgcgcgaccg gcagccggcg gggcgccggg gatggcgcag cagggaggcg gggagcacga
     2221 agacagggat ggcagcgagg gcaagcacga tccgagaacc ggccggcgtg accgccgccg
     2281 agcgcgccgg gcagatgggg ccgtggattg tcgccctgct ggtcgcgttc accctggtgc
     2341 gcggcgtcat ctgggcctat gccatcccgc cgctgaacgc gcccgacgag ccgtcacacc
     2401 tcatcgccgt gagccagatc cgcgccctgc gcgggctgcc ggtggcgatc ctgatcgacc
     2461 ccaacacgct gagcgaaaat agttcgccgc gcccgccgga ggtgctggcc tatctcgccc
     2521 agccgcagtt ccactatacg cgcttccgcg ccctgtccta cgagtcgacg cagccgccgc
     2581 tgtaccacct gctgtgcgcc gtgctgaccc ggcccctgcc ggcggacccg gagacgctgc
     2641 tatatggctg ccggctggtg tcggcgctgc tgggcgcgct gacggtgttc gccgtggcgc
     2701 gcgccgccgc gacgctggcc ccgcgccaac cggcgttcgc gctgggcgcg ccgctggcgc
     2761 tgacgctctg gccgcagttc gggttccaga cggccacgct gaacaacgac atcgccagca
     2821 cgctggcggg cgcggcgctg gcctgggcgt gggccgcggc gatgcgccgg cccggcgacc
     2881 ggcgctggac cgccgggctg ggcgcgctga ccgggctggg gatgctctgc aagctgaccg
     2941 tcgccgccac cctgcccggc accgggctga tcttgctggg ccgggcgcgc gccgggggcg
     3001 acacgctgcg gcggttcgtg ggcgaggccg tgctggccgg ggcggtggcc gcggtgccgg
     3061 tgctgccgtg ggtgtggcgc aatctcgcgg tctacggcga gccgaccggc gcggcgcaga
     3121 tgctgagcgt gatccacagc atttacacca cgcgcctggg cctgccggcg gagacgcgct
     3181 tcatcatacc gccgctgggc gactttgtct ggtacagctt tagctcgtgg tggggcgtgt
     3241 tcggctggcg cagcgtgttt ctgccggggc cgctctacgt ggtggccgcc gggctgacgc
     3301 tgctcggcgc gggcggcgcg acgacctggc tggtgcgccg ggtgcggacg cggcagccgc
     3361 cggcccccgc gcagggctgg ccgctggcgg cgtgcgccgt gaccatcgcg gcggccctgg
     3421 cgaactacat cctctacaca gtgaccacgg acgcctcgtt gcaggggcgc tacacattcg
     3481 tggcgctggc ggcggtgtgt ctggtgggcg tgagcg
//
LOCUS       DASUYC010000827         2474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1850555, whole genome shotgun sequence.
ACCESSION   DASUYC010000827 DASUYC010000000
VERSION     DASUYC010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1850555"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..897
                     /locus_tag="VF276_13520"
     CDS             136..897
                     /locus_tag="VF276_13520"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6607922.1"
                     /translation="MTTTHGLLPEKPDLAARCKGKRVVICDDFPMNRLNWRSVAENLG
                     CEVVGETATGEEALELLERLRPDLLLLDFNLAGKLNGRDVQREARRRTLGTRVLLITA
                     QGGSPGFFAWIHQEDGPDGALDKMCSNHEIKTAIAHLLTSNTRYLSPQILDSDPHYHR
                     NPLAGLTRAELQVLRHVAEGEKLYDIAQRYAVSPLTIRAYMNDIYCKLGVHPQTLLAA
                     ALVYERWIAVTPLPPADEAAPDAGPEGVTEEQAAP"
     gene            894..1874
                     /locus_tag="VF276_13525"
     CDS             894..1874
                     /locus_tag="VF276_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HEX6607923.1"
                     /translation="MKIVIPDDYQDAVRTLDCFAKLRSHEVVIYHDTVKDLDTLAARF
                     AEADALVLIRERTAISAALLERLPRLKLISQTGRGVPHIDLAACTRRGVAVAAGGGSP
                     YAPAELTWALILAGMRRLPQEMAALKAGRWQTSLGLGLRGRVLGIFGFGRIGSLVAGY
                     GRAFEMQVLVWGRETTRARAQAAGFAVAADQAAFFAQADVLSLHLKLTPETAGIAGPA
                     DLARMKPTAGLVNTSRAGLIVPGALEEALRAGRPGWAAVDVYEDEPALHDPLLALDNA
                     LCTPHLGYVEKDSYELYFDRAFDQVLAFAAGQPINLINPAVLAATPPAGA"
     gene            complement(1989..>2474)
                     /locus_tag="VF276_13530"
     CDS             complement(1989..>2474)
                     /locus_tag="VF276_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007162241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HEX6607924.1"
                     /translation="YRSIEFRFETIDAPVHQPTGTVNYPNDFGYTRVTEFKHLSGQER
                     AKTSIVYEYPEAEGDPYYPVPRPENAELYKRYKALADATPGVYFVGRLATYKYYNMDQ
                     VVAQALAVYRKVLMPQLQPAIVQEPAASFAALVKRTPAPSLPASLTAIESIDTTGAGA
                     Q"
BASE COUNT          412 a          835 c          817 g          410 t
ORIGIN      
        1 ggcacggctc tgccggcacg gcatcccaac acttgtgggg tcaaaatacc cacacttgtg
       61 gactaacaaa atcgctgatt gtgtgctacg atagtcctca taatcgcacc caatctagac
      121 ggaggtgtca tcaccatgac aacgactcac ggattactac cggaaaaacc ggacctggcc
      181 gcgcggtgca aaggcaaacg ggtggtcatt tgcgacgatt tcccgatgaa ccgcctgaac
      241 tggcgttccg tcgccgaaaa cctgggctgc gaggtggtcg gggagacggc cacgggcgaa
      301 gaggcgctcg aactgctgga gcgcctgcgc ccggacctgc tcctgctcga tttcaatctc
      361 gccggcaaac tgaacgggcg cgatgtgcag cgcgaagccc ggcgccgcac cctgggcacc
      421 agggtgctcc ttatcacggc ccagggcggg tcccccggtt tctttgcctg gatccaccag
      481 gaggacggcc ctgacggtgc gctggacaaa atgtgctcga accatgaaat aaaaacggcc
      541 atcgcccacc tgctgaccag caacacccgg tatctgtcgc cccagatcct ggatagcgac
      601 ccgcactacc accgcaaccc gctcgccggc ctcacccgtg ccgaattaca ggtgttacgc
      661 catgtggccg agggcgagaa gctgtacgac attgcccagc ggtatgccgt gtcgcccctg
      721 accatccgcg cctacatgaa tgacatctac tgcaagctgg gcgtccaccc gcagacgctg
      781 ctggccgcgg cgctggtata cgagcgctgg atcgccgtca ccccgctacc gcccgcggat
      841 gaggccgccc cggatgccgg cccggaaggt gtcacggagg aacaagccgc tccatgaaaa
      901 tcgttatccc ggacgactac caggatgccg tgcgcacgct cgattgcttc gccaagctcc
      961 ggagccacga ggtcgtcatc tatcacgaca cggtgaagga tctcgacacg ttggccgcgc
     1021 gttttgccga ggccgacgcg ctggtgctga tccgtgagcg cacggcgatc agcgcggcgc
     1081 tgctggagcg cctgccccgg ctcaaactga tcagccagac cgggcgcggg gtgccgcata
     1141 tcgacctggc ggcctgcacg cggcgcgggg tggccgtggc cgccgggggc ggctcgccct
     1201 atgcgcccgc cgagctcacc tgggcgctga tcctggccgg gatgcgccgg ctgccgcagg
     1261 aaatggccgc gctcaaggcg ggccgctggc agacgagcct gggcctgggc ctgcgcgggc
     1321 gcgtgctggg catcttcggc tttggccgca tcggcagcct ggtcgcgggc tacgggcgcg
     1381 cgttcgagat gcaggtgctg gtctggggcc gcgagaccac gcgcgcacgg gcgcaggcgg
     1441 cgggcttcgc cgtggccgcg gaccaggcgg cgttcttcgc gcaggccgac gtgctcagcc
     1501 tgcacctcaa gctgacaccc gaaacggcgg gcatcgccgg ccccgcggat ttggcgcgca
     1561 tgaagcccac ggcgggcctg gtcaacacca gccgggccgg cctcattgtg ccgggcgcgc
     1621 tggaagaggc gctgcgcgcg gggcgccccg gctgggccgc cgtcgatgtc tacgaggacg
     1681 agccggcgct acacgatccc ctgctggcgc tggacaacgc cctctgcacg ccgcacctgg
     1741 gatatgtaga gaaagacagc tacgagctct acttcgaccg tgcgttcgat caggtgctgg
     1801 cctttgccgc aggccaaccg atcaatctga tcaaccccgc agttctggcc gccaccccgc
     1861 ccgccggggc gtaggtgcat ccctaccccg gcgggcgtgt ctactatcgt caagggagat
     1921 gtaatacgtg ggcctgggca ccgcccgctt tatgccggca ccattggccg tgagctgcag
     1981 aagccccgct attgcgcccc cgcgccggtg gtgtcgatgc tctcgatcgc ggtgagcgac
     2041 gccggcaagc tgggcgcggg cgtgcgtttg accagcgcgg cgaagctggc cgccggctcc
     2101 tgcacaatcg ccggctgcaa ctgcggcatg agcaccttgc gatagaccgc cagcgcctgg
     2161 gccaccacct ggtccatatt gtagtatttg tacgtcgcca gccgccccac gaagtagacg
     2221 ccgggcgtcg cgtcggccag cgccttgtag cgcttgtaga gctcggcgtt ctccgggcgc
     2281 ggcaccgggt agtacgggtc gccctcggct tcgggatact cgtagacgat gctcgttttc
     2341 gcgcgctcct gaccgctcag gtgcttgaac tcggtgacgc gcgtgtagcc gaagtcgttc
     2401 ggatagttca ccgtgcctgt cggttgatgg accggtgcgt cgatcgtctc gaagcggaac
     2461 tcgatcgagc ggta
//
LOCUS       DASUYC010000828         5885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_878779,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000828 DASUYC010000000
VERSION     DASUYC010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5885
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878779"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(273..713)
                     /locus_tag="VF276_13535"
     CDS             complement(273..713)
                     /locus_tag="VF276_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007982180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6607925.1"
                     /translation="MTLQRMDNVLIVVEDLEAVKAFFVALGMEVEGETTVEGSSVDRL
                     IGLPNVRATLALLRTPDGHGRIELDKFHTPDAIRTGPANVPVNALGIRRIMFAVAGID
                     EVVARLRAHGAELVGEVVQYEDTYRLCYVRGPEGIIVGLAEQRG"
     gene            complement(774..1301)
                     /locus_tag="VF276_13540"
     CDS             complement(774..1301)
                     /locus_tag="VF276_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015148143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6607926.1"
                     /translation="MLPLETERLVIREMRRADVESLHLLFSDPLFMRFWPVFDRSRTE
                     RWVEESLLDYARYGFGLWALTLRGSDDAIGDCGLGVINGLEGTEGTEGNGQIQIGWHV
                     RRDLWGQGLATEAALACRDYAFDRLGLPALSALIQPENAASRRVAEKIGMTLLRQVRH
                     RGRMICLYGMQRPQP"
     gene            complement(1342..2079)
                     /locus_tag="VF276_13545"
     CDS             complement(1342..2079)
                     /locus_tag="VF276_13545"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEX6607927.1"
                     /translation="MRQDATERRGARWKLDPGRVRTHLLLGAITVAGCALTYVLQPRF
                     WPTLTLTIAFGYLALLFLAVTLLIGPLQLARRRRNPVNIHSRRDTGIWAGLTGCLHVV
                     FGLQGHLGGQILAYFFTPAGGLRLDLLGVSNDLGAAATVILVGLAALSNDWTLRRLRG
                     PRWKFWQRFNYALFLLVLAHTLGYQTVIPRDPIMRPLVVVLALGILAAQLWGRYRYAN
                     RARRAPVAPAQPSVAGIREPVTDKVLK"
     gene            2234..2896
                     /locus_tag="VF276_13550"
     CDS             2234..2896
                     /locus_tag="VF276_13550"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX6607928.1"
                     /translation="MAQRTIILVRHGQTDLSTAPIDKLGNGLTVLGIQQAELTGRRLA
                     GLPVTAIRHSTARRAQQTAEVIAAQFPGVPVQGDPVLLECIPAYPPAFVEWYSGVAGA
                     PPAADTVPAAMRFWLTLWPPGTPWPVMAAEVAQAAAAFDRYFGPPPEDAADAHEIIVC
                     HGNIIRYFMLRALQASPELWVHADIYNCGICEIVVRGERGTQLLSFNDSGHLPAELRM
                     FF"
     gene            2908..3882
                     /locus_tag="VF276_13555"
     CDS             2908..3882
                     /locus_tag="VF276_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6607929.1"
                     /translation="MVPPWLHFIERPFPSANMVLIAGPRPILVDPGFGSDWAATADLL
                     RAAGIPPERLALIVNTHYHSDHIGGNYALQHAYGLPVAAHATEAAQVNARDPQTGMAV
                     WLDQPIEPYTVQQPLRDGDEIDTGAVRLRVVHTPGHTRGHIALYAPAEGILIAGDALH
                     AADVPWINRFGEGADALERALATLDRLERLPLRWVCSGHGPAITDPAAALAKTRRRYT
                     QWQQAPEQLAWHACKRIFAYALMIRDGLPEAEVRPYLLSRAWFADYSRYVFGVPPEDF
                     VERFLAEILRVGAGVWQDGRLVARTPHIPPPPGWLAAPARPQDWPACD"
     gene            complement(3887..4207)
                     /locus_tag="VF276_13560"
     CDS             complement(3887..4207)
                     /locus_tag="VF276_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607930.1"
                     /translation="MTQLPLAELWDDKGLVQATKLRDIGIDEIKDLLRTGSVSVVVAD
                     VGESLRWIEASQTYSFWKTEVAPHLYQESKPYLENYPGEYFYFATEWRLATGRAIIVL
                     EKEH"
     gene            complement(4246..5160)
                     /locus_tag="VF276_13565"
     CDS             complement(4246..5160)
                     /locus_tag="VF276_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX6607931.1"
                     /translation="MTEIPENFARTMGEVYGEEGRAWLERLPDLLAACARRWRLTVGP
                     PFVPLSYNYVAPATQADGTSVVLKVGYPGRELRTEIAALQLYDGHGIAQLLDADAAAG
                     ALLLERLLPGTPLAQLADDAAATTIAAQVMRQLWRPVPPDHPFPTVADWAAGLGRLRA
                     RFGGTTGPLPAPMVDQAERLFADLLGSMDTPVLLHGDLHHDNILMAQRQPWLALDPKG
                     LVGEAAYETGALLRNPAGRVLKAPDPHAMMARRIDQLAAELGFERERIRAWALAQAVL
                     SAWWSIEDHGYGWEDAIAYAEILDGLRH"
     gene            5348..5749
                     /locus_tag="VF276_13570"
     CDS             5348..5749
                     /locus_tag="VF276_13570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018332567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX6607932.1"
                     /translation="MSAFTPAEIAYLQSQRLGRLATVNAAGEPHVVPVAFRYNPDLDT
                     IDIGGHTIGNTKKFRDVARDGRVAFVVDDVLPPWKARGLEIRGRAETLPTGGQEIMRD
                     FGPEMIRLTPTRIVGWGIEGETFQANSRAAG"
BASE COUNT         1006 a         2054 c         1859 g          966 t
ORIGIN      
        1 acggctgttg ctgggatagc gcgaggcacg cgcagccccg cgcggagtag gggcacgcgc
       61 tgctctgccg gccagcgatc ctctcaggcg cgaatcggta taacgaataa cgagcattat
      121 ttgtcataac tgctgatcag acaccgcgag aacctagtgc cagcaacccc gacaggccgt
      181 agtgttgcgc ttgttcctgg ggcacgctac ccgacaccgg gtttgggcat gttgctgccc
      241 ggttgcccaa gatttaggcg accaggcacg ccttaccccc gctgctcggc aagcccgaca
      301 atgatgccct cggggccgcg gacgtaacag agccgatacg tgtcctcgta ctgcaccact
      361 tcgccaacca gttcggcacc gtgggcgcgc aggcgcgcga cgacctcgtc gatcccagcg
      421 acggcgaaca tgatgcggcg gatcccgagt gcgttcaccg gaacgttcgc gggtcccgtt
      481 cggatcgcgt ccggcgtgtg gaacttgtcc agctcgatcc gtccgtggcc gtccggcgtg
      541 cgcagcagcg cgagggtcgc acggacgttc ggaaggccga tgagccgatc taccgaggag
      601 ccctcgacgg tcgtctcgcc ctctacctcc ataccaagcg cgacgaagaa cgccttaacc
      661 gcctcaaggt cttcgaccac gatgagcacg ttgtccatcc gttggagcgt catgtctttt
      721 cccccatttg actcgaaaca cgcagcatgg gcggtttatc tggttctccg ccttcagggc
      781 tgcgggcgtt gcatgccata gaggcagatc attctgccgc gatgccggac ctgcctgagc
      841 aaggtcatgc caatcttctc agccacacgg cgcgaggcgg cgttctccgg ctgaatcagc
      901 gcgctgagcg ccggcaggcc cagacggtcg aacgcgtagt cacgacaggc cagcgctgcc
      961 tccgttgcca ggccctggcc ccacaggtcg cggcgcacat gccagccgat ctggatctgg
     1021 ccgttacctt ccgtaccttc cgtcccttca agcccattga tgacgcccag gccgcaatcg
     1081 ccgatcgcgt catcgctgcc ccgcaaggtg agtgcccaca gcccgaaccc gtaccgggca
     1141 tagtctagca agctctcctc tacccatcgc tcggtcctgc tgcgatcaaa gaccggccag
     1201 aagcgcatga agagtggatc cgagaaaagc aggtgtaggc tctccacgtc cgcacgtctc
     1261 atctcgcgta tgaccagacg ctcggtctcc aaaggcagca tcgcgaacct ccgtgagcgg
     1321 gctgaatctc tcagaccttg ttcattttag aactttgtcc gtgaccggtt ctcgtatacc
     1381 tgctactgaa ggctgcgccg gggcaaccgg cgcccgccgg gcacggttgg cgtagcggta
     1441 gcggccccag agttgcgccg ccaggatgcc cagggccagc accaccacca gcggacgcat
     1501 gatcgggtcg cgcggtatca ccgtctggta gcccagcgtg tgcgccagca ccagcaggaa
     1561 caacgcgtag ttgaagcgct gccagaactt ccagcgtggc ccgcgcagcc ggcgcagcgt
     1621 ccagtcgttc gagagggccg ccagccccac caggatcacc gtagccgccg cgcccaggtc
     1681 gttgctcacg ccaagcaggt ccagccgcaa cccgcccgcc ggggtaaaga aataggccag
     1741 gatctggccg cccaggtggc cttgcaagcc aaagacgacg tgcaggcacc ccgtcagacc
     1801 ggcccagatg ccggtgtccc ggcggctatg gatattcacc gggttgcgcc gccggcgcgc
     1861 aagttgcaat ggcccgatca gcagcgtgac ggccaggaag agcagcgcca ggtagccaaa
     1921 cgcgatggtg agggtcagtg tgggccagaa gcgcggctgc aagacatatg tgagcgcgca
     1981 ccccgcaacg gtgatcgccc ccaacaacag atgtgtccgc acccgaccgg ggtccagctt
     2041 ccaccgcgcg ccgcgccgct cagttgcgtc ctgcctcacg attccgatcc tcctgggcag
     2101 ataacgacgc gggtcgccga tccggcctta tcgcacgggc gaccttgacg cgcgcgcgca
     2161 ccgggcgtaa actcaagact gagttcggcc ccgcccggtg ccggagaaga atcgcgattg
     2221 gagaacccgc acgatggcgc agcgcacaat catcctcgtc cggcatggac aaaccgacct
     2281 gagtaccgcg cccatcgaca agctgggcaa cggcttgacg gtgctgggca tccagcaggc
     2341 cgaattgacc gggcggcggc tggcgggcct gccggtgacg gcgatccgcc acagcacggc
     2401 ccggcgagcc caacagacgg cggaggtgat cgccgcgcag tttcccggcg tgccggtgca
     2461 gggcgacccg gtgctgctcg aatgcattcc ggcctatccg ccggcctttg tcgagtggta
     2521 cagcggcgtc gcgggcgcgc cgcccgccgc cgataccgtg cccgcggcaa tgcgcttctg
     2581 gctcaccctg tggccgccgg gtacgccctg gccggtgatg gccgcggagg tcgcgcaggc
     2641 cgcagccgcc tttgaccgct atttcgggcc gccgcccgag gatgccgccg atgcgcacga
     2701 aatcatcgtc tgccacggca acatcatccg ctacttcatg ctgcgcgctt tgcaggcgtc
     2761 gccggaactc tgggtccacg ccgacatcta caattgcggc atctgcgaga ttgtggtgcg
     2821 tggtgagcgc ggcacccaac tgttgtcgtt caacgacagc ggccacctac ccgccgagtt
     2881 acgcatgttc ttctagaagg ttaccacatg gttccgccct ggctgcattt tatcgagcgc
     2941 cccttcccga gcgcaaacat ggtcctcatc gccgggccac gacccatcct ggtcgatccc
     3001 ggcttcggca gcgactgggc ggccaccgcg gacctgctgc gcgcggcggg catcccgccc
     3061 gaacggctgg ccctgatcgt caatacgcat tatcacagcg accacattgg cggcaactac
     3121 gccctgcaac acgcctacgg cctgccggtg gccgcccacg cgaccgaagc ggcccaggtg
     3181 aacgcccgcg atccgcagac cggcatggcc gtctggctgg accagcccat cgaaccctat
     3241 actgtccagc agccgctgcg cgacggcgac gaaatcgaca ccggcgccgt gcggcttcgc
     3301 gtggtgcata cgccggggca cacccgcggg cacattgcgc tctacgcgcc cgccgagggg
     3361 atcctgatcg cgggcgacgc cctgcacgcc gccgatgtgc cctggatcaa ccgcttcggc
     3421 gagggcgcgg atgcgctgga gcgggcgctc gccacgctcg accgcctgga acgcttgccg
     3481 ctgcgctggg tctgttccgg ccacggcccg gccatcaccg atccggcggc ggccctcgcc
     3541 aagacccggc ggcgctatac gcagtggcag caggcgcccg aacaactggc ctggcacgcc
     3601 tgcaagcgca tcttcgccta cgccctgatg atccgcgacg gcttgccgga agccgaggtc
     3661 cggccctatc tactcagccg cgcctggttc gccgactaca gccgatacgt cttcggcgtg
     3721 cccccggagg acttcgtcga gcgcttcctg gccgagatcc tgcgcgtggg cgcgggcgtc
     3781 tggcaggacg ggcggttggt ggcgcgcacg ccgcacatcc caccgccgcc tggctggctc
     3841 gcggcgcccg cccggcccca ggattggcct gcttgcgact agagaatcag tgctcttttt
     3901 ccagcacgat tatggctctg cctgtcgcca gcctccactc tgtggcaaaa tagaaatact
     3961 caccagggta gttttccaga taaggtttgc tttcctggta cagatgcggc gcaacttccg
     4021 tcttccagaa gctatatgtt tggcttgcct cgatccatcg caaggattct cctacatcgg
     4081 ctaccacgac tgacacggat ccagtccgaa gcaagtcctt gatttcgtct atccctatat
     4141 cacgcagctt ggtcgcctgt actaaaccct tgtcatccca caactcagcg aggggaagct
     4201 gggtcacaat tctatcggaa ctcctcactg ttacccccgt ctcatttaat gtcgcaggcc
     4261 atccagaatc tcggcgtagg cgatcgcatc ctcccagccg tagccgtggt cctcgatgct
     4321 ccaccaggcc gacagcacgg cctgggcgag cgcccaggcg cgaatccgct cccgctcgaa
     4381 gcctaactcg gcggccagtt ggtcgatgcg ccgcgccatc atggcgtggg gatcgggcgc
     4441 cttcagcacg cgccccgccg gattgcgcag cagcgctccg gtctcgtagg ccgcctcgcc
     4501 caccagcccc ttggggtcca gggccagcca gggctggcgt tgcgccatca ggatattatc
     4561 gtggtgcagg tcgccatgca gcagcaccgg cgtgtccatg gaaccgagca ggtcggcaaa
     4621 cagccgctcc gcctgatcca ccatgggcgc gggcagcggt ccggtggtgc cgccgaagcg
     4681 agcgcgcagg cggcccaggc ccgccgccca atcggccacc gtggggaacg ggtggtcggg
     4741 cggcacgggg cgccacaact gccgcatcac ctgggcggcg atggtcgtgg cagccgcgtc
     4801 gtcggccagt tgcgccagcg gcgtgccggg caggaggcgt tcgagcagca gcgcgcccgc
     4861 cgcggcgtcg gcgtcgagca gttgggcgat gccgtggccg tcgtagagtt gcagcgctgc
     4921 gatctccgtg cgcagttcgc ggccagggta gcccaccttc agtaccacgg acgtgccgtc
     4981 cgcctgcgtc gccggggcca cgtagttgta cgacagcggc acgaacggcg gccccacggt
     5041 caggcgccag cgccgggcgc acgcggccag caggtccggc aggcgctcca gccaggcccg
     5101 gccctcctcg ccgtacactt ctcccatcgt ccgcgcgaaa ttctcgggaa tctccgtcat
     5161 ggtgtcctcg cagattgctc accgcgaagg cgcgaagagc gcgaagaagc ggtgcattca
     5221 tgaacggtgc cgggtggccg gctagcatac atccgcctat attatagatc gcggaacaat
     5281 gttgaggccg tttactgcct gtatggctgc ccgcagcatt acgcgtaggg gggagaaagg
     5341 aaacgccatg agcgccttta caccggcgga gatcgcctat ctccagagcc agcggctagg
     5401 ccggctcgcc acggtcaacg cggcgggcga accccacgtc gtgccggtgg ctttccgcta
     5461 taatccggac ctggacacga ttgacatcgg cgggcacacg atcggcaata ccaagaagtt
     5521 ccgcgatgta gcccgcgacg ggcgggtggc cttcgtggtc gacgatgtgc tgcccccgtg
     5581 gaaggcccgc gggctggaga tccgggggcg ggccgaaacc ctgcccaccg gcggccagga
     5641 gatcatgcgc gacttcggcc ccgaaatgat ccgcctgacg cccacgcgca tcgtcggctg
     5701 gggcattgag ggcgagacgt tccaggcgaa cagccgcgcc gccggctgag aaagggaagc
     5761 ccatgcagat cgaatcgcga ctggccgcgc ttggcctggt gctcccggcg cccgtccagg
     5821 tggcacccga tgtgcgcctg ccctttgccc aggtgcgcat ccgcggcaac cgtgcctaca
     5881 tcgcc
//
LOCUS       DASUYC010000829         2746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_825303,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000829 DASUYC010000000
VERSION     DASUYC010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825303"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="VF276_13575"
     CDS             <1..561
                     /locus_tag="VF276_13575"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEX6607933.1"
                     /translation="QSLEEFLRLLRLHQVDVLVDVRSQPFSKYTVQFNAPALKDAVTA
                     AGLRYLYLGKELGGRPEELDYYDAEGHVLYGRVAEAAWFLDSIARLEKGRQQYRVAIM
                     CSEENPAGCHRRLLVGRVLAARGTPVQHIRGDGRLQTEAELEQEEAGPDTGAIQPSLF
                     DTAEVTVWRSIRSVLRKGTPPTSSEL"
     gene            585..938
                     /locus_tag="VF276_13580"
     CDS             585..938
                     /locus_tag="VF276_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEX6607934.1"
                     /translation="MDVRLNNSSQLAGFTKQDNLPFFLKEICGAEYVHEPLLAPTQEM
                     LDAYKKQKGSWADYERQFLALMAERRVEDHIDRAGFAVPTVLLCSETTAEQCHRRLVL
                     EYLQAKWGDIEIVHL"
     gene            950..1657
                     /locus_tag="VF276_13585"
     CDS             950..1657
                     /locus_tag="VF276_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607935.1"
                     /translation="MRIVVSHLTRMNPGYICVAGLDLDTGAHIRPVLNSTRLATAMLR
                     RCGGIFDIGSVIDLGTTSYQGRRPETEDHVFSFKQARHAGMVAAPAFWQLLRQTSQAH
                     LTDIFGPELKRIDHTCAVDPGKGQASLGCLAAERPALRVDKYNKIRIGVTTNPALPPL
                     DLPVTDIRLYETDQQTPRYEVIEDLAARIAEGTSVLLGVGLSRAYMKMGDTTARHWLQ
                     VNNIHLADDPLFTDRER"
     gene            complement(1775..>2746)
                     /locus_tag="VF276_13590"
     CDS             complement(1775..>2746)
                     /locus_tag="VF276_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEX6607936.1"
                     /translation="EHDPERLMEVEERISLLHELKRKYAPTIEEIIEFGRQAAAELDT
                     LTHSEERIAELLEEEAALLAEAGALAQALSERRQEAGERLAGTVEQAMRELLMGSIQF
                     ATSITRTPDPHGLPAPGPDGQPGTWAFDTRGIDRVEFLISPNPGEPLKPLSKIASGGE
                     TSRLMLAMKSILSAADHTPTLIFDEIDAGVGGRSGQVVGEKLWSLTDHHQVLCITHLP
                     QIAAFADSHFRITKHVKNDRTSTQVDELAASDRTHEIAAMLGGTPVTSSSLENAREII
                     RHIEQSKALRNGATASDLLATAGAASLPPEAGNNGHTPAWPRIAEHS"
BASE COUNT          451 a          879 c          955 g          461 t
ORIGIN      
        1 cagtcgctgg aggagtttct gcgcctcttg cggctgcacc aggtggacgt gctggtcgat
       61 gtgcggtcgc agccgttctc caaatacacc gtgcagttca acgccccggc gctcaaggac
      121 gccgtcaccg ccgccggctt gaggtatctc tacctgggca aagaactggg cgggcgcccg
      181 gaagaactcg actattacga cgccgagggc catgtgctgt atgggcgcgt ggccgaggcg
      241 gcgtggttcc tcgacagcat tgcgcgactg gagaagggcc ggcaacagta ccgggtggcg
      301 atcatgtgca gcgaggagaa ccccgccggg tgccatcggc ggctgctggt gggccgggta
      361 cttgccgcgc ggggcacgcc ggtgcagcac atccgcggcg acgggcgcct gcaaaccgag
      421 gcggaacttg aacaggaaga agccggcccg gacaccggcg ccatccaacc ctcactcttt
      481 gataccgcgg aggtcacggt atggagatct atacgatcgg ttttacgaaa aggaacgccg
      541 ccgacttctt cggaactctg agagcggcgg ggatcaagcg cctcatggac gtgcggctca
      601 acaattcgtc gcaactggcc gggttcacga agcaagacaa cctgccgttc ttcctcaagg
      661 aaatctgcgg cgcggagtac gtgcatgagc cgctgctggc cccgacccag gagatgctcg
      721 acgcctataa gaagcagaag ggcagttggg cggattacga gcggcagttc ctggcgctca
      781 tggccgagcg ccgggtcgag gaccacatcg accgcgccgg gttcgccgtg ccgaccgtgc
      841 tgctgtgcag cgagacgacc gccgagcagt gccaccggcg gctggtgctg gagtatctcc
      901 aggcgaagtg gggcgatatc gagatcgttc acctgtagga gacacaggca tgcgcatcgt
      961 ggtgagccac ctgacccgca tgaaccccgg ctacatctgc gtggccggcc tcgacctgga
     1021 caccggggcg catatccgcc cggtgctgaa cagcacccgg ttagccacgg cgatgttgcg
     1081 ccgctgcgga ggtatcttcg acatcgggtc ggtgatcgac ctgggcacga ccagctacca
     1141 ggggcggcgg cccgaaaccg aggaccatgt cttttcgttc aagcaggccc gccatgccgg
     1201 catggtggcg gctccggcct tctggcaact gctccggcaa acgtcgcagg cgcacctgac
     1261 cgacatcttc ggcccggaac tcaagcgcat cgatcatacc tgcgcggtcg atccggggaa
     1321 ggggcaggcg tcgctgggct gtctggccgc cgagcggccc gccttgcgcg tggacaagta
     1381 caacaagatc cgcatcggcg tgacgaccaa cccggccctg ccgccgctgg acctgcccgt
     1441 gaccgacatc cggctctacg agaccgatca gcagacgccg cgctacgagg tcatcgagga
     1501 tctggccgcg cgcatcgccg agggcaccag cgtgttgctg ggggtgggcc tcagccgcgc
     1561 ctacatgaag atgggtgaca ccacggcacg ccactggctc caggtgaaca acatccacct
     1621 ggccgacgac ccgctcttca ccgaccgcga acgttagtgg cgcgtcagag tgatgcggct
     1681 gggcggcagg ccggtttcaa accggcccct acaccgcaaa agcacagccg gtttgaaacc
     1741 ggcccctaca ccgcaaaagc gcagtggccg tctgctagct gtgctcggcg atgcgcggcc
     1801 aggcgggggt atggccgttg ttgccggcct ccggcggcag gctggccgca cccgccgtcg
     1861 ccagcaggtc gctggccgtg gcgccgttgc gcaacgcctt actctgctcg atgtggcgga
     1921 tgatttcgcg ggcgttctcc aggctgctcg atgtgaccgg cgtgccgccg agcatggccg
     1981 cgatctcgtg ggtgcggtcg gacgccgcca gttcgtccac ctgggtgctg gtgcgatcgt
     2041 tcttcacgtg cttggtgatg cggaagtgcg agtcggcgaa ggcggcgatc tgcggcaggt
     2101 gggtgatgca gagcacctgg tggtggtcgg tgagcgacca gagcttttcg ccgaccacct
     2161 gcccgctgcg cccgccgacg cccgcgtcga tctcgtcgaa gatcagcgtc ggggtgtggt
     2221 cggcggcgga gaggatcgac ttcatcgcca gcatgaggcg cgaagtctcg ccgccggagg
     2281 cgattttgga gagcggcttg agcggttcgc cggggttggg cgagatcaga aactccacgc
     2341 ggtcgatgcc gcgggtgtcg aaggcccagg tgccgggctg gccgtcgggg ccgggcgcgg
     2401 gcaggccgtg cggatcgggc gtgcgggtga tgctggtggc gaactggatg ctgcccatca
     2461 gcaactcgcg catggcctgt tcgaccgtgc cggccagccg ctcgcccgcc tcctggcggc
     2521 gctccgatag ggcctgggcc agcgcgcccg cctcggccag caaggcggcc tcttcctcca
     2581 gcagttcggc gatacgttct tcactatgag tgagtgtatc cagttcggcg gcggcctggc
     2641 ggccaaactc gatgatctcc tcgatggtcg gggcgtactt gcgcttcagt tcgtgcagca
     2701 ggctgatgcg ctcttcgacc tccatcaggc gctccgggtc gtgctc
//
LOCUS       DASUYC010000830         2508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_44432,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000830 DASUYC010000000
VERSION     DASUYC010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44432"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..455)
                     /gene="uraH"
                     /locus_tag="VF276_13595"
     CDS             complement(96..455)
                     /gene="uraH"
                     /locus_tag="VF276_13595"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009555250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HEX6607937.1"
                     /translation="MPGRLTTHVLDTAQGRPAAGMRIELHALDLSGEWSLITTVRTNA
                     DGRTDAPLMAGEQFARGLYEIVFFVGEYFAAQGVATAKPPFLDRVPVRFGIAEADGHY
                     HVPLLTSPWAYSTYRGS"
     gene            complement(460..1383)
                     /gene="pucL"
                     /locus_tag="VF276_13600"
     CDS             complement(460..1383)
                     /gene="pucL"
                     /locus_tag="VF276_13600"
                     /inference="COORDINATES: protein motif:HMM:TIGR03383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004846 - urate oxidase activity [Evidence
                     IEA];
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urate oxidase"
                     /protein_id="HEX6607938.1"
                     /translation="MSATIRYGKRQVTVYRTYAQPLQGLTPIPESAYTGRDNTLFAVD
                     VDVEVYGDNFLPAYTEGDNSNVVATDTMKNFVMHQALAYQGATMEGFLAFLGEQFLAT
                     YPQMQRLRVIGKEQPFQAAPVPGASGFGPSNVLFSRGHDDYAVASLDIERDGTGVRIT
                     DHRCCQVGMQLIKVTGSSFTRFVRDDYTTLPERVDRPLFIYLDAFWKYNDVTDLVSSD
                     LTRYVAPAQMRDLIGYVFHDFVSQSIQHLVYEMGQRILARFPQLAEISFEAQNRLWDT
                     MAVSETDERIKVYCDPRPPYGSIGLTLTRED"
     gene            complement(1460..1984)
                     /gene="uraD"
                     /locus_tag="VF276_13605"
     CDS             complement(1460..1984)
                     /gene="uraD"
                     /locus_tag="VF276_13605"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HEX6607939.1"
                     /translation="MPRTSLTLSDLNAMDRAEFAAALAAVFEGAPAFVAQAWDARPFA
                     TVAALHQALVAAMHAAGAEQQVALIAAHPDLVGRAALAGTLSRESTQEQAAAGLDRLT
                     PDEIATFTRLNTAYRDRFGFPFVICARENKKESILAGFATRLEHTRPQEIDAALAEIA
                     KIAALRLADLIAPA"
     gene            2165..>2508
                     /locus_tag="VF276_13610"
     CDS             2165..>2508
                     /locus_tag="VF276_13610"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6607940.1"
                     /translation="MISDSTTAVVESPAQQPMAGRATVLALSFAAFVMIGANDGGTGV
                     LLPSLQASYGVDKATIGLIFLAATGGYLCAAFSSGLLVERLGIRRFLLLGTLMFILGA
                     GTLSLQVPFLGLL"
BASE COUNT          384 a          787 c          894 g          443 t
ORIGIN      
        1 caggaccggg tgaggcaccc ggcttcctcg ctgatcgcgc ccaggatctc gcaccgcacc
       61 aggatcttgc gtgccgctgc ttcgtcgatc actcgttagc tccctcgata ggtgctgtag
      121 gcccacggcg aggtgagcaa cggcacatga taatggccat cggcctcggc gatgccgaag
      181 cgcacgggca cgcgatccag gaaaggcggc ttggccgtcg ccaccccttg cgcggcaaag
      241 tattcgccca cgaaaaacac gatttcgtac agaccgcggg caaattgctc gcccgccatt
      301 agcggcgcgt ccgtacggcc gtccgcattg gtgcgcaccg tggtaatcag gctccactcg
      361 ccggatagat cgagcgcgtg aagttcgatc cgcatgcccg cggcgggccg gccctgcgcg
      421 gtatctagaa catgggtggt aagacgaccg ggcatcgggc tagtcctcgc gcgtcagggt
      481 caggccaatc gagccgtagg gcgggcgcgg gtcgcaatag actttgatgc gctcgtcggt
      541 ctcggacacg gccatcgtgt cccagaggcg gttttgcgcc tcgaaggaga tctcggccag
      601 ttgcgggaag cgcgccagga tgcgttgccc catctcgtag accaggtgct ggatcgactg
      661 gctcacgaaa tcgtggaaca catagccaat cagatcgcgc atctgggccg gcgcgacgta
      721 gcgggtcagg tccgatgaga ccaggtcggt gacatcgttg tacttccaga aagcatccag
      781 gtagatgaac aaaggccggt ccactcgctc cggcagggtc gtgtagtcgt cgcgcacgaa
      841 gcgcgtgaac gaactgcccg ttactttgat cagttgcatg ccgacctggc agcagcggtg
      901 atcggtgatg cgcacgcctg tcccgtcccg ctcgatatcc aggctggcga cggcgtagtc
      961 gtcgtggccg cggctgaaca gcacgttgct ggggccgaac ccgctcgctc ccggcacagg
     1021 cgccgcttga aacggctgct ccttgccgat cacgcgcagc cgctgcatct gcgggtaggt
     1081 cgccaggaat tgctcgccca ggaaggccag gaagccttcc atggtcgcac cctggtaggc
     1141 cagcgcctga tgcatgacaa agttcttcat tgtatccgtc gccacgacgt tactgttatc
     1201 gccctcggtg taggcgggca gaaagttgtc gccgtacact tccacgtcca catccacggc
     1261 aaagagcgtg ttgtcgcgcc cggtgtaggc cgactcgggg atgggggtga ggccctggag
     1321 gggctgcgcg taggtgcgat agacggtgac ttgccgtttg ccgtagcgga tcgttgcact
     1381 catggtcggt tcctcacttc tttgtgggtc ttgggccggg cggcggcgcg gataggcggc
     1441 cccggcggcg ggatgggggt caggccggcg cgatgaggtc ggccagccgc agcgcggcga
     1501 tcttggcgat ctcggccagg gccgcgtcga tctcctgggg gcgggtgtgc tccaggcggg
     1561 tggcgaaccc ggccaggatg ctctctttct tgttctcgcg cgcgcagatg acaaagggaa
     1621 agccgaagcg gtcgcggtag gcggtgttca gccgggtaaa cgtggcgatc tcgtcgggcg
     1681 tgagccggtc gaggcccgcc gccgcctgct cctgcgtgga ctcgcggctc agggtgccgg
     1741 ccagggcggc gcggcccacc aggtcggggt gggcggcgat gagcgcgacc tgctgctccg
     1801 cccccgcggc gtgcatggcc gccaccagcg cctggtgcag tgcggccacc gtggcgaacg
     1861 ggcgcgcgtc ccacgcctgg gccacgaagg ccggcgcgcc ttcaaacacg gcggccagcg
     1921 cggcggcgaa ctccgcgcgg tccatggcgt tcaggtcaga taaggtgaga gatgtcctgg
     1981 gcatgggtct cctctgccgg ggtgcgcgtt aactttgtgc atcccggtgg gcgagcaacc
     2041 tctcccccga cccctccccg ccgcggggag gggagtgacg gtcctgcgcg ccggcgattc
     2101 cccctgcccg ccgcgggaag gggggcaggg gggttaggtt gttcgggcag caggaggagg
     2161 agcggtgatt agcgattcga cgacggcggt ggtggagagc cccgcgcaac agcccatggc
     2221 ggggcgggcc acggtcctgg ccctgtcttt cgccgcgttt gtgatgatcg gggccaacga
     2281 cggcggcacc ggcgtgctct tgccgagcct gcaagcctcc tatggggtgg ataaggccac
     2341 catcggcctg atcttcctgg ccgcgacagg cgggtaccta tgtgccgcgt tcagcagcgg
     2401 cctgctggtg gaacggctgg gcatccggcg cttcttgctg ctgggcacgc tcatgttcat
     2461 cctgggggcg ggcacgctga gcttgcaggt gccgttcctc ggcctgct
//
LOCUS       DASUYC010000831         3065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_863826,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000831 DASUYC010000000
VERSION     DASUYC010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863826"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VF276_13615"
     CDS             complement(<1..380)
                     /locus_tag="VF276_13615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607941.1"
                     /translation="MAQDGQAEQQGGQGPRNRRLWLAGGGLTVLILGLIILISPLSPF
                     NNTPTPTPGPGETPVATPSPGTPGTPPPSPTPGPTAVPTIIYSYTGGDSRPHVSGITA
                     GVGMAGYDLAGAVPIAVSFSAAMNT"
     gene            716..925
                     /locus_tag="VF276_13620"
     CDS             716..925
                     /locus_tag="VF276_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607942.1"
                     /translation="MDNDELTERVLAAEEKLGEAYQLLGEVRDELKAAGRKKDYQALN
                     EVLDRLGRYGRMLGEMRSSWGDPLD"
     gene            976..2508
                     /locus_tag="VF276_13625"
     CDS             976..2508
                     /locus_tag="VF276_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HEX6607943.1"
                     /translation="MPPDPAVIEAYSRFFAAPPPAAPDSEPEAAPAGATEALALFHEA
                     ARRVPAYAHFLAAQGCDSAGIRTPADFARVPVMDKANYLRQYPLPDLCWDGDLSRLHM
                     ISVSSGSTGQPFFWPRGFRQELATAGNFEEIYRYIFQAHERRTLVVVCFAMGTWIAGT
                     FTYQATALIAQKGYPITVVTPGLNRDEILRAVRELGPYYEQVVLAGYPPFVKDVLDAG
                     RAAGIDWAAFHIKMIWAGEVFSEEWRAHVLEIVGSDNPLSATSALYGSADAAVLGHEW
                     PLSVAIRRFAAAHPDLTHKVFGEARLPSLLQYDPRARYFEQVNGELVFTAPTGTPLIR
                     YNIHDNGGILSCAQLVAWCRSEGYDPEADLAAHGCPVAPELPFVYIFGRSDFTISFYG
                     AKVYPENVKTGLEDPRVRAAVTGKFVMYTTYDAQQRERLAIHVELAPGDAPDTADLLA
                     LLGTVLPEHLRRQNSEFANYTPADAQLPFIELHATGDPAWFAPGLKHRYTRPPERGKV
                     ST"
     gene            2505..2777
                     /locus_tag="VF276_13630"
     CDS             2505..2777
                     /locus_tag="VF276_13630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607944.1"
                     /translation="MINPTEFVKGEQYTNRKGTYTVVELHPPTMTVRFEDGTVETLDI
                     KIQQRILSNMTLPPRTPEPPPRARTGSRTAAKPAAKAKTPAPATAD"
BASE COUNT          514 a         1030 c         1019 g          502 t
ORIGIN      
        1 gtgttcatcg ccgccgagaa ggacacggcg atgggcaccg cgcccgccag gtcatagccg
       61 gccatgccga cgcccgcggt gatgccgctg acatggggcc ggctgtcgcc gccggtgtag
      121 ctgtagatga tcgtcggcac cgccgtgggg ccgggcgtgg gcgagggcgg gggcgtgccc
      181 ggcgtgccgg gcgagggcgt ggcgaccggc gtttcgccgg gccccggtgt gggcgtgggg
      241 gtgttgttga aaggcgagag cgggctgatg aggatgatca gccccaggat cagcacggtc
      301 aggccgcccc cggccagcca gaggcgcctg ttacgcggcc cctggccgcc ctgctgttcc
      361 gcttgtccgt cctgtgccat actgccggcc tccgtggcgg cgcctgtcat ggcgcctgct
      421 ggggcgatcc gccgctcgga ccaggcggga tcgcgctgtt ttggttgttg cggtctggtc
      481 gatgtgctac aatcaaagag gcaaagggtt ctcgcattgc cgcgctgccg aatcgcacgc
      541 gcgatcggtc tcatcgatat gacgccggac accggcgcga ggttcccgtt ccttccgttg
      601 tggtacgagg ttgcgcgggc cgggcccgcg aggatggcgg caccgcgggt ggctggaccg
      661 gacccgccgg gtgcggatgt attgatttat tatagcataa ggatagcgcc gggttatgga
      721 taacgacgaa ctgaccgagc gcgtgctggc cgccgaggaa aagctgggcg aggcctacca
      781 gttgctcggc gaggtgcgcg atgaactcaa ggccgcgggc cgcaagaagg actaccaggc
      841 gttgaacgaa gtgctcgacc gcctgggccg ctatggccgc atgctcggcg agatgcgatc
      901 cagttggggc gacccccttg attaagcccc gcgcgtggcc tgggcacccg caggcccgcc
      961 gccaggagat tcgtgatgcc gcctgatccc gcagtgatcg aagcctacag ccggtttttc
     1021 gccgcgcctc cgcctgccgc gcccgacagc gagccggagg ctgcgcccgc cggggcgaca
     1081 gaagccctgg ccctgttcca cgaagccgcc cggcgcgtgc ccgcctatgc gcactttctc
     1141 gccgcccagg gttgcgactc cgccggcatc cgcacgcccg ccgatttcgc ccgcgtgccc
     1201 gtcatggaca aggccaacta tctgcgccaa tacccgctgc ccgacctctg ctgggacggc
     1261 gatctcagcc gcctgcatat gatctcggtc tcgtcggggt ctacggggca accgttcttc
     1321 tggccgcgcg gcttccgcca ggaactggcg accgccggca attttgagga gatctaccgc
     1381 tatatcttcc aggctcacga gcggcgtacg ctggtcgtcg tctgctttgc catgggcacc
     1441 tggatcgccg ggacgttcac ctaccaggcc acggcgctca tcgcgcagaa gggctacccg
     1501 atcacggtgg tcacgcccgg cctgaaccgc gacgagatcc tgcgcgcggt gcgcgaactg
     1561 gggccgtact acgagcaggt ggtgctggcg ggctatccgc ccttcgtcaa ggatgtgctc
     1621 gacgccgggc gcgccgcggg cattgactgg gccgccttcc acatcaagat gatctgggcg
     1681 ggcgaggtct tcagcgagga gtggcgcgcc catgtgctgg agatcgtcgg gagcgacaac
     1741 ccgctgagtg cgacatccgc actctacggg tcggcggacg ccgccgtgct cggccacgaa
     1801 tggccgctgt cggtggcgat ccggcgcttc gccgccgctc accccgacct gacccacaag
     1861 gtcttcggcg aggcccgcct gccgtcgctg ctgcaatacg acccgcgcgc ccgctatttc
     1921 gagcaggtta acggggagct cgtgttcacc gcgcccaccg gaaccccgct gattcgctat
     1981 aatatccacg ataacggcgg catcctgtcg tgcgcccaac tggtggcctg gtgccgcagc
     2041 gaaggctacg accccgaggc cgacctggcg gcgcacggct gccccgtggc acccgaattg
     2101 ccgtttgttt acatctttgg acggagcgat ttcacgatct cgttctacgg ggccaaagtc
     2161 tatccggaga acgtgaaaac cggactggaa gacccgcggg tgcgcgcagc ggtcacgggc
     2221 aagttcgtga tgtatacgac ctacgacgcc cagcagcgcg aacggctggc gatccacgtc
     2281 gaactggcgc ccggcgacgc gcccgacacg gcggatctcc tggcgctgct cggcactgtg
     2341 ctgccggagc acctgcggcg gcagaattcg gagttcgcca actatacacc ggcggacgcg
     2401 caactgccgt tcatcgaact gcacgcgacc ggcgacccgg cctggtttgc ccccggcctg
     2461 aaacatcgct atacccgccc acccgaaagg ggaaaggttt cgacatgatc aaccccaccg
     2521 aatttgtaaa aggcgagcag tacaccaacc gcaagggcac ctataccgtg gtcgagttgc
     2581 acccgcccac gatgaccgtg cgcttcgagg acggcacggt ggagacgctg gacatcaaga
     2641 tccagcagcg tatcctgagc aatatgacgt tgccgccccg cacgcccgag ccgccgcccc
     2701 gcgcgcgcac cgggtcgcgg accgccgcca aaccggcggc caaggccaag acacccgcgc
     2761 ccgcaacggc agactaagcc cgcggcgccc atccctttgt cactctgagc gcagcgaaga
     2821 gtctcgtcgc gtaggggaac aagacggaga ttcttcgctc cgctcagaat gacaaagcag
     2881 gttgacggtc cagtgagtaa gcccgcggcg acccggctgg aggaacgtag ctaatcgtga
     2941 ctaaccttac tgaatttgtt gtcggccagc agtacaccaa ccgcaagggc acctttaccg
     3001 tggtcgagtt gcacccgccc acgatgaccg tgcgcttcga ggacggcacg gtggagacgc
     3061 tggac
//
LOCUS       DASUYC010000832         2898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1886009, whole genome shotgun sequence.
ACCESSION   DASUYC010000832 DASUYC010000000
VERSION     DASUYC010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1886009"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..1143
                     /locus_tag="VF276_13635"
     CDS             112..1143
                     /locus_tag="VF276_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX6607945.1"
                     /translation="MKAVMKTAPGVGNIEVREIREPDPPPGHVKIAVQAAGICGTDIH
                     IYYDEFRTRPPVVLGHEIAGQVVEVGAGVTSVEPGDRVTTETYFSTCGNCLFCRSGRA
                     NLCPDRRSIGSAVDGGFTHYVIVPARNVHRLPDNVDYQAGALTEPLACVVHGALELPR
                     VAPGALAVITGPGAIGLLTLQVVKAAGATAVLLGTDADEARLALGRELGADYALNIQR
                     EDAGDQIAALSEGLGADIVYECSGAGPAAQTLLEFVRRGGQYAQIGLFGKPVAWALDQ
                     VCYKELTVTGSNASVPSAWRTALRLLAAGQVRTAPLVSGVYPLTEWRTAFDVFEQRTG
                     IKTLLSPVE"
     gene            1213..2115
                     /locus_tag="VF276_13640"
     CDS             1213..2115
                     /locus_tag="VF276_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014369516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX6607946.1"
                     /translation="MTQHTGTPAAPAAAPDIPELKRIAAQVRGDVLRAVANAGGGHIG
                     GPFSAADILVALYFRIMRIRPDDPTWEDRDRFVLSKGHSSIGLYATLAARGYFPREEL
                     MTFDKIDSRLQGHPDMTRLPGLDMSTGSLGIGISTAVGMAIGAKRRGKDLFVYCIVGD
                     GECQEGEVWEAAMIGGRYKLDNLIGILDFNGLQQYGWPGATLAQRLAPWGREELPALW
                     RAWGWRVLEMDGHDMQAILDTLNEAKGGRGSGQPTMVVAHTVKGQGVSYTRGAYEWHA
                     KVPTADELARGLAELEAAGEIDHD"
     gene            2108..>2898
                     /locus_tag="VF276_13645"
     CDS             2108..>2898
                     /locus_tag="VF276_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX6607947.1"
                     /translation="MTEQKSLREAFGEILSDLGAENPDVLVLDGDLANSTRADIFAER
                     VPGQFLEMGIAEQNMMGVAAGLATMGFIPFISTFACFAVKRALDQVRVVIAQPSLNVK
                     ITGGYSGILTGKTGKTHQAVEDISVMRAMPHMTTLVPADAVELRGMLRAAVATPGPMY
                     IRLTRDPSPIIFDSSYQFAIGKAVVLREGQDVALISTGVETTRALAAADLLAADGVSV
                     HVLHVPTIKPLDEAAIIDAARRTGRVVTAEDHTILGGLGGAVAETL"
BASE COUNT          480 a          990 c          994 g          434 t
ORIGIN      
        1 gctgccccgg tagccggcca cggtgatatt gcggggtagg ggccggtttc aaaccggctc
       61 gccgcccatt agacgcgcgc gtccgtgcct tcgctgaata ggagaataca tatgaaagcc
      121 gtcatgaaaa ccgcgcccgg cgtgggcaac atcgaggtcc gcgagattcg cgaacccgat
      181 cccccgcccg gccatgtcaa gatcgccgtc caggcggcgg gcatctgcgg caccgatatt
      241 cacatctact atgacgagtt ccgcacccgc ccgccggtcg tgctcggcca cgaaatcgcc
      301 gggcaggtgg tggaggtggg cgcgggcgtg acgagcgtgg agcccggcga ccgcgtgacc
      361 accgagacct acttctcgac gtgcggcaac tgcctgttct gccgcagcgg acgggccaat
      421 ctctgcccgg atcgccgctc catcggctcg gcggtggacg ggggctttac ccactacgtc
      481 atcgtgcccg cgcgcaacgt ccaccggctg cccgataacg tcgattacca ggccggggcg
      541 ctgaccgagc cgctggcctg cgtggtgcat ggcgcgctgg aactgccccg cgtggcgccc
      601 ggcgcccttg ccgtgatcac cgggccgggg gccatcggtt tgctgacgtt gcaggtggtc
      661 aaggcggcgg gggccaccgc cgtgctgctg ggcacggacg ccgacgaagc ccggctggcg
      721 ctgggtcgcg aattaggcgc cgactacgcg ctgaacatcc agcgcgagga cgccggggac
      781 cagatcgccg cgctcagcga gggcctgggc gccgatattg tctacgagtg ttcgggggcc
      841 ggacccgcgg cacaaaccct gctcgaattt gttcgccggg gcggccagta cgcccagatc
      901 ggactcttcg gcaagcccgt cgcctgggcg ctcgaccagg tatgctacaa ggaactgacc
      961 gtcaccgggt cgaacgccag cgtgcccagc gcctggcgca cggcgttgcg cctgctggcc
     1021 gcgggtcagg tccgcacagc gcccctggtg tcgggagtct acccgctgac cgagtggcgg
     1081 acggcgttcg acgtattcga gcagcgcacc ggcatcaaga cgctgctcag cccggtggaa
     1141 taaaccggat ccgccggcgc ctggggcgcg caccgcatcc cggcccggcc cgctgcatgc
     1201 gaaggagcat ttatgacgca acataccggc accccggcgg cccccgccgc cgcgcccgac
     1261 atacccgaac tgaaacgcat tgccgcccag gtgcgcggcg acgtgctgcg cgcggtcgcc
     1321 aacgccggcg gcgggcacat cggcggacct ttctcggcgg cggatatcct ggtcgccctc
     1381 tacttccgca tcatgcgcat ccgaccggac gatccgacct gggaagatcg cgaccgcttt
     1441 gtgctctcca agggccactc cagcatcggc ctctatgcca cgctggcggc gcggggctat
     1501 tttccgcgcg aggagttgat gaccttcgac aagatcgatt cccgcttgca gggccacccc
     1561 gatatgaccc gcctgccggg gctggacatg tccaccggct cgctcggcat cggcatttcc
     1621 accgccgtcg gcatggcaat cggcgccaaa cggcgcggca aggatctctt cgtctactgc
     1681 atcgtcggcg acggcgagtg ccaggagggc gaggtctggg aagcggccat gatcggcggg
     1741 cgctacaaac tcgacaacct gatcggcatc ctcgacttca acgggctgca gcagtacggc
     1801 tggccgggcg ccaccctcgc tcagcggctg gcgccgtggg gccgggaaga gttgcccgcg
     1861 ctctggcggg cctggggctg gcgggtgctc gaaatggacg gccacgacat gcaggccatc
     1921 ctcgacacgc tgaacgaggc caagggcggg cgcggcagcg ggcagccgac catggtggtc
     1981 gcccacacgg tgaaaggcca gggcgtgtcg tatacccgcg gggcctacga atggcacgcc
     2041 aaggtgccga ccgccgacga actggcgcgc ggcctggccg aactggaagc agcgggagaa
     2101 atcgaccatg actgagcaaa agagcttgcg cgaggccttt ggcgagatcc tatccgacct
     2161 gggagccgaa aaccctgatg tgctcgtgct cgacggcgac ctggcgaaca gcacccgcgc
     2221 cgacatcttc gccgagcgag tccccggcca gtttctggag atgggcatcg ccgagcagaa
     2281 tatgatgggc gtcgcggcag gcttggcgac catgggcttt atcccgttca tcagcacctt
     2341 cgcctgtttc gccgtcaagc gcgcactcga ccaggtgcgc gtggtgatcg cgcagccgag
     2401 cctgaacgtc aagatcaccg gcggctacag cggcatcctc accggcaaga ccggcaagac
     2461 gcaccaggcg gtggaagaca tcagcgtgat gcgcgccatg ccccacatga ccacgctggt
     2521 gcccgccgac gccgtggagt tgcgcggcat gttgcgggcg gcggtggcta cgcccggccc
     2581 gatgtatatt cgcctgacgc gcgaccccag cccgatcatc ttcgacagca gctaccaatt
     2641 cgccatcggc aaggcggtgg tgctgcgcga gggccaggac gtggcgctga tcagcaccgg
     2701 ggtggagacc acgcgcgccc tcgccgccgc cgatctgctt gcggcggacg gcgtcagcgt
     2761 ccatgtgctg cacgtgccga cgataaagcc gctggacgag gcggccatca tcgatgcggc
     2821 gcggcgcacg ggccgcgtgg tcaccgcgga ggatcacacg atcctgggcg gcctgggtgg
     2881 cgcggtggcc gaaacgct
//
LOCUS       DASUYC010000833         4164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1748555, whole genome shotgun sequence.
ACCESSION   DASUYC010000833 DASUYC010000000
VERSION     DASUYC010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4164
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1748555"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1554)
                     /locus_tag="VF276_13650"
     CDS             complement(<1..1554)
                     /locus_tag="VF276_13650"
                     /inference="COORDINATES: protein motif:HMM:NF023260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HEX6607948.1"
                     /translation="MKAEAALVNPAPAAPQPQAPPPAPPPAPASYGYTAQPPAYGYGA
                     PQDQPVPPAPPAGYGYNAPTGGQAPQSGYGAPPAQGGYGAPAPAPPTYTYGAPPAPGY
                     STPPPSDTARVAAAPSMTPPAPRKSHLVRNVLLVFLLLAVVGVGAGAYFLNQTFNKTA
                     VPAARLLPADVYGYLTVSTHPDSAQKASMDKLQAAFKDQPGFQAAWDKIFKETGDSTA
                     AVKSTLGDCSDTTPTADANGWDTLNSYLGNNATVALLPLTKGELAQLSEGSGDPEKML
                     LPKVLAFVDLDFNPLNKKGLLPKLKQAGDKPADMPLVEKYRDIEIRKLPPSQCAGSDS
                     EPPTVYVTMIDGTAAVGFDVVPLRGVIDHYKDGHSLQDNAAFQAMQAELPKDRLGTLY
                     LNMTSIYSTIQSVMPADMAAENPAANFQLDGTAAIVLTAHDDGLQVDTVSDLKMNGLA
                     GSGNALGTAVVNDVPAGSWAFYTGTDLKTTIEQTLAMLRKQGQAQEVDDALAQVKEQM
                     GIDVEHDLLP"
     gene            complement(1699..2709)
                     /locus_tag="VF276_13655"
     CDS             complement(1699..2709)
                     /locus_tag="VF276_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEX6607949.1"
                     /translation="MVESLTPSPAPGNDALPIALADVQAAHARVRAAIVRTPLLRLRG
                     PGIPETASVWLKPENLQLTGSFKLRGAYNKLASLSAEERAHGVIAYSSGNHAQGVACA
                     AHLLGIHTTIVMPEDAPPVKLAATRGWGADVVLCAPGSEARRELAEALQREHGYVLVP
                     PYDDPLIMAGQGTATLEILEDLPDLDLLVLPCGGGGLLSGNTTALKGLRPQARIIGVE
                     PELAADANASLAAGHIVQWTGAQTGRTVADGLRTQSVGQRPWQVISRLVDSLTTVSEA
                     AILDGVRYLARYAHLVVEPSGAVPLAAVLSGQVDVTSGSVALLISGGNIEPSALAAIL
                     AG"
     gene            3026..3340
                     /locus_tag="VF276_13660"
     CDS             3026..3340
                     /locus_tag="VF276_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607950.1"
                     /translation="MDDELNRARDNAQATGEEMANKAEHAGHKAEHESHGLVDDIMNL
                     GPEAGHAVAEGAHDLARGIHDAVTGLFHHDKEAPHVEPAAPSSTEPAVPPAGPAEDDN
                     VV"
     gene            3475..>4164
                     /locus_tag="VF276_13665"
     CDS             3475..>4164
                     /locus_tag="VF276_13665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607951.1"
                     /translation="MPPRSQSKIMYVCQQCGSESPKWQGRCPDCGEWNTYTEITVQGA
                     SAAAVNRAAALLRTDGGKPVPLPEVSADAFPRLVVPGSEFNRVLGGGVVPGSLTLMSG
                     DPGIGKCLTAESRVLDPTTGDFLPITAWHDQPRPVLAVNTDTLRLDTAQVSMFHDRGV
                     HPIVELTTELGRKLRCTPSHPVLTPHGWHPVSALRAGDRVAAPRALPYFGNESLPEHV
                     VKLIAYVLSDGS"
BASE COUNT          666 a         1316 c         1482 g          700 t
ORIGIN      
        1 cggcagcaga tcgtgttcga cgtcgatgcc catctgctct ttgacctggg ccagggcgtc
       61 gtcaacctcc tgggcctgac cctgcttgcg cagcatcgcg agcgtctgct cgattgtcgt
      121 cttgaggtcg gtgccggtgt agaaggccca gctccccgcg ggcacgtcgt tcaccacggc
      181 ggtgccgagc gcgttgccgc tgcccgccag cccgttcatc ttcaggtcgc tgaccgtatc
      241 gacctgcaac ccgtcgtcgt gggcggtgag cacgatggcg gcggtgccgt caagctggaa
      301 gttggcggcg ggattctcgg cggccatatc agccggcatg accgactgga tggtgctgta
      361 gatcgaggtc atgttgaggt agagcgtgcc caggcgatcc ttgggcagtt cggcctgcat
      421 ggcctggaag gcggcgttgt cctgcaggga gtggccgtcc ttgtagtgat cgatcacgcc
      481 gcgcagcggc accacgtcga agccgaccgc cgccgtgccg tcgatcatgg tgacatagac
      541 ggtcggaggc tcgctgtcgc tgcccgcgca ctggctgggg ggcagcttgc ggatttcgat
      601 atcgcggtac ttctcgacca gcggcatatc ggcgggcttg tcgccggcct gcttcagctt
      661 gggcagcagg cccttcttgt tcaacgggtt gaagtcgagg tccacgaaag ccaggacctt
      721 gggcagcagc atcttctccg gatcgccgct gccttcgctc agttgggcca gttcgccctt
      781 ggtcagcggc agcagggcga cggtggcgtt gttgcccagg taagagttca gggtgtccca
      841 accgttggca tcggcggtgg gcgtggtgtc gctgcaatcg cccagggtgg acttcaccgc
      901 ggcggtgctg tcgccggtct ccttgaagat cttgtcccag gcggcctgga agcccggctg
      961 gtccttgaac gccgcctgga gcttgtccat ggacgccttc tgggcgctgt cggggtgggt
     1021 ggagacggtg aggtagccat agacatcggc gggcagaagg cgcgccgcgg gcaccgccgt
     1081 cttgttgaag gtctggttca ggaagtaggc cccggcgccg acgccgacca cggccagcag
     1141 caggaaaacg agcaggacat tgcgcaccag gtggctcttg cgcggggcgg gcggggtcat
     1201 tgacggggcg gccgccaccc gcgcggtatc cgagggcggg ggcgtgctat agccgggcgc
     1261 gggcggcgca ccgtaggtat aggtgggcgg ggccggcgcc ggggcgccgt agcccccctg
     1321 cgcgggcggc gcaccatagc cggactgcgg ggcctggccg ccggtgggag cgttgtagcc
     1381 gtagccggcg ggcggcgcgg gcgggacggg ctggtcttgc ggcgcgccat agccgtaggc
     1441 gggcggttgg gccgtgtagc cataagatgc gggcgcgggc ggcggtgcgg gcggcggggc
     1501 ctgcggttgc ggcgcggcgg gcgccgggtt gaccagggcg gcttccgctt tcatgggccg
     1561 gccgcacccg gcgcaaaact tcgcggtctc ggtattctga gcaccacact gcgggcagaa
     1621 catgggtcgg gactctccta tcttgaatga tgcagatcga atgatgccaa agccggtgag
     1681 aagaaaaggc gggcagcctt atcccgccag aatggcggcc agcgcggatg gttcgatgtt
     1741 gccgccactg attaatagcg ccaccgagcc ggaagttacg tccacctggc cgctcagcac
     1801 cgccgccaac gggacggcgc cggaaggctc gaccaccagg tgcgcatagc gcgccaggta
     1861 gcgcaccccg tcgaggatgg ccgcttcgct caccgtggtc aggctgtcga ccaggcggct
     1921 gatcacctgc cacggccgct gcccgaccga ctgcgtgcgc agcccatcgg ccacggtgcg
     1981 gcccgtttgc gcgcccgtcc actggacgat gtgcccggcg gccagcgacg cgttggcgtc
     2041 ggcggccagt tccggctcga cgccgatgat ccgagcctgg ggccgcaggc ccttgagcgc
     2101 ggtggtgttg ccgctgagca gtccgccgcc gccgcacggc aacaccagca ggtcgagatc
     2161 gggcagatcc tccaggattt cgagcgtggc cgtaccctgc ccggccatga tcagcggatc
     2221 atcgtagggc ggcaccagca cgtagccgtg ctcgcgctgc aacgcttcgg ccagttcgcg
     2281 ccgggcttcg gagccgggcg cgcagagcac cacgtcggcg ccccagcccc gcgtggcggc
     2341 cagcttcacc ggcggggcat cttcgggcat gacgatggtg gtatggatgc cgagcagatg
     2401 ggccgcgcag gccacgccct gcgcatggtt gccgctcgaa taggcgatca cgccgtgcgc
     2461 ccgctcttcg gcgctcagcg aggccagttt gttgtaggcg ccgcggagct tgaaggaccc
     2521 ggtcagttgc agattctccg gcttcagcca gacggacgcc gtttccggga tgccggggcc
     2581 gcgcagccgc agcagcggcg tgcgcacaat cgccgcccgc acgcgcgcat gggcggcctg
     2641 cacatcggcc agggcaatgg gcagcgcgtc gttgccgggc gccggcgagg gggttaggga
     2701 ttcgaccatt gattcctttc caggctgcac gaggctcgta agctctcggc gtggtacgtg
     2761 gttctccgat agcaggggcg gtcgcgtagc cggtggggtt ttctgtatcg aaggcggata
     2821 gtttcactat aaatctacag gattctcgat ctagaggcaa tagatgaacg gtcataattc
     2881 gggctatcgc gcatagtact tttgggtggt tccggcgggc aggccgccgc gggacggctt
     2941 ggacagccca cgccaacagg gtacgtttcg tgctacaggt cgcttggcgg cctatcgccc
     3001 ctgttagtat aaaggagaga gaaccatgga cgatgaactg aaccgcgcca gggataacgc
     3061 ccaggcgacg ggcgaggaaa tggcgaacaa agccgagcac gccggccaca aggccgagca
     3121 tgagagccac ggcctggtcg acgatatcat gaacctggga cccgaagcgg gccacgccgt
     3181 ggcggaaggc gcacacgacc tggcgcgtgg catccatgac gccgtgaccg ggttgttcca
     3241 tcacgacaag gaagccccgc acgtggagcc cgcggcgcca tccagcacgg aaccagcggt
     3301 cccgcccgcc ggtccggctg aggacgacaa cgtcgtctaa gtagccgccg ctatccgcga
     3361 ggccggtcgc acgggctgcg accggccttg ccgtgtatag cacgggccgg gcggacatgg
     3421 tataatagca gaatagaaca tttggtcgcc gccagcctag caggagagcc acgcatgccc
     3481 ccccggtcgc aaagcaagat catgtacgtc tgccagcagt gcggcagcga atcgcctaag
     3541 tggcagggcc gctgccccga ttgcggcgaa tggaacacct acaccgagat cacggtgcag
     3601 ggcgcctcgg cggccgctgt gaaccgggcc gcggccctgc tgcgcaccga cggcggcaag
     3661 ccggtgcccc tgcccgaggt cagcgccgac gccttcccgc gcctggtggt accgggcagc
     3721 gagttcaacc gcgtgctggg cggcggggtc gtccccggct cgctgacgct gatgagcggg
     3781 gatccgggaa ttgggaaatg cctgaccgcc gaaagccggg tgctggaccc cacaacgggt
     3841 gacttcctac caattaccgc atggcacgac cagccccgcc ctgttttagc ggtgaatacg
     3901 gacaccttac gtctcgatac ggctcaagtc agcatgttcc atgaccgggg ggtgcatccc
     3961 attgtggaat taacgactga gctagggcgc aagctgcgct gtacgccctc gcatcctgtg
     4021 ctcacaccgc atggatggca tcccgttagc gcgttgcggg cgggcgatcg cgtcgcggcc
     4081 ccacgagcgc tgccgtattt cggcaatgag tcgttgccgg agcacgtcgt caagctaatc
     4141 gcttatgtgc tttccgatgg atcg
//
LOCUS       DASUYC010000834         3103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1043777, whole genome shotgun sequence.
ACCESSION   DASUYC010000834 DASUYC010000000
VERSION     DASUYC010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1043777"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..644)
                     /locus_tag="VF276_13670"
     CDS             complement(117..644)
                     /locus_tag="VF276_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX6607952.1"
                     /translation="MSREIDAPVLVLNQNYEPLNVCNVRRALVLVFNGKAEVLELNHA
                     IIHSPMHNIHAPSVIRLAHMIRRPHPRAKLSRREVFLRDDYTCQYCGVHTRDLTLDHV
                     MPKSRGGPHTWENLVSACRACNHRKGGKTIEEARMRPLHMPRQPKANIYHLVYRYVQA
                     APLEEWHKFLPGLSS"
     gene            889..1902
                     /locus_tag="VF276_13675"
     CDS             889..1902
                     /locus_tag="VF276_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607953.1"
                     /translation="MIPDLAVLILKIRDYLVWLLPAYAAAILLTAALNTALARPASDA
                     PRDPATPHSPGTPTAWLLARLLGGGPPPGALLALWAGTLTRALTTLALILTAAHDPLL
                     AGLRLALALILSAGVAILAGRAATPAAPMPPAGGADPRARSATPPPPPGANPYVTPAV
                     GADLRARPAAPPPPLLPGVPNLPDPAAAPSSRIPAWARALWQEASRLVDQTAGPLFAA
                     ALIGGLFTAWDPARAGVRALAGLGPWAAAPVAALTLLLPLPAGTDLPLIAALTTYNVP
                     LPALLALMGGAPLFHAAQVRAVAAQYGRPAAALYLLGGLLLLIGLSMLLGTLFGGGLL
                     PVL"
     gene            complement(1922..2386)
                     /locus_tag="VF276_13680"
     CDS             complement(1922..2386)
                     /locus_tag="VF276_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607954.1"
                     /translation="MRANVAPAAYLTALLLTALGVAGTHALFSRLLLYASYWWFNPLF
                     GSSSDTGLQVLFLLAPLPAAGVAAFLLRRDGGWAFLVGGALLVHGLVRVIDAAATMEL
                     SRAVVQPSDLYWLYLSMVYGVLAGLGALGGLVWQCRVLGARRPRFSFAGRNQ"
BASE COUNT          514 a         1115 c          963 g          511 t
ORIGIN      
        1 accactgacc actgaccact cgtactcagc actcagcact cagcactaag gaaagagccg
       61 cccggccccg aaaggcggca ggcggctcgg atccggtggc gggcagccac cggcgactaa
      121 ctactcaggc ccggcaggaa cttgtgccat tcctccagcg gggcggcctg cacatagcgg
      181 tagaccaggt gatagatatt ggccttgggc tggcgcggca tgtgcagcgg gcgcatgcgc
      241 gcctcctcaa tcgttttgcc gcccttgcga tggttgcaag cgcggcacgc cgataccaga
      301 ttctcccagg tgtgtggccc cccacgcgac ttgggcatca catggtcgag cgtgagatcg
      361 cgggtatgca cgccgcaata ctggcaggtg taatcatcgc gcaagaagac ttcgcgccgc
      421 gagagcttgg cccgcgggtg cgggcgccgg atcatgtggg ccagccggat caccgacggg
      481 gcatggatgt tgtgcatcgg cgagtgaatg atcgcgtggt tcaactccag cacttcggcc
      541 ttcccgttga acacaaggac caacgcccgc ctgacattgc acacgttgag cggttcgtaa
      601 ttctggttca ggaccagtac cggcgcgtcg atttctcttg acacggttag ctccgtcttc
      661 tctagcggcc cacacagcgg tctacatact gcggtctcca gctccttgcg caaactccgg
      721 cacaaccggg aagggcggga ccggggtccc gcctcatgcg tataattgta tcatgttcta
      781 ggcgattcga caatgggaaa tgcgagggat tatgtctact cttgccgatg gggacgtgct
      841 cccctgccgg acggacccga ccgcagcgcc gcgcagaaac gggggcctat gatacccgat
      901 ctagccgtcc tcatcctcaa gatccgcgac tacctggtct ggctgctgcc cgcctacgcc
      961 gcggccatcc tgctgaccgc cgcgctgaac accgccctgg cccgccccgc cagcgatgcg
     1021 ccacgcgatc cggccacgcc ccacagcccc ggcaccccca cggcctggct gctggcgcgc
     1081 ctgctgggcg gcgggccgcc gcccggtgcc ctgctcgccc tctgggccgg cacgctgacc
     1141 cgcgccctca cgaccctcgc cctcatcctg acggcagccc acgacccgct gctggccggg
     1201 ctgcggctgg ccctggccct gatcctcagc gcaggcgtag ccatcctcgc cgggcgtgcc
     1261 gccacgcccg ccgccccgat gccgcctgct ggaggggccg acccgcgggc ccgctcagcc
     1321 accccaccgc cgccccccgg cgcgaatcca tacgtcacgc ctgctgtagg ggcagacctg
     1381 cgggcccgcc cggccgctcc accgcccccg ctgctccccg gtgtgcccaa cctcccggac
     1441 cccgccgccg cgccatcctc ccgcataccg gcctgggcgc gcgcgctgtg gcaggaagcg
     1501 agccggctgg tggaccagac cgccgggccg ctgttcgccg ccgcgctcat cggcgggctg
     1561 ttcaccgcct gggacccggc ccgcgccggg gtgcgcgccc tggccggact ggggccgtgg
     1621 gccgccgcgc cggtggccgc gctcaccctg ctgctgccgc tgcccgccgg cactgacctc
     1681 ccgctcatcg ccgccctcac cacctacaac gtgccgctgc ccgcgctgct ggcgctcatg
     1741 ggcggcgcgc cgctgttcca cgccgcccag gtccgcgccg tcgccgcgca gtacgggcgc
     1801 cccgcggcgg ccctctatct cctcggcggg ctgctcctgc tgatcgggct gagtatgcta
     1861 ttgggtacgc tgttcggcgg gggattgctt ccggtgcttt aaactctata cagagaggcg
     1921 gctactgatt gcggcctgca aaggagaaac gcgggcggcg cgcgccgagc acccggcact
     1981 gccagaccag cccgcccagc gcgcccaacc ccgccagcac accatagacc atgctcaggt
     2041 agagccagta gaggtccgac ggctgcacca ccgcccggct cagttccata gtggccgccg
     2101 cgtcaattac gcgcacgagg ccgtgcacga gtagcgcgcc ccctacgagg aacgcccatc
     2161 ccccatcccg ccgcagcagg aaggcggcca cgcccgcggc gggcagcggg gccagcagaa
     2221 agaggacctg caagccggtg tcgctactcg acccgaacag cgggttgaac caccagtagc
     2281 tggcatagag caagagccgg ctaaacagcg catgcgtccc ggccacgccg agcgcggtca
     2341 gcagtaacgc agtgaggtag gcagcagggg caacgtttgc gcgcatcgtc tcgtccttcc
     2401 tggcaggaat tacactgcta cctttctaac gtcgcagcac gcaccgggtt gcatcttggt
     2461 tctcgatgct aaaccatagc aaggcgcatc cagcagctaa cgattcttgg ctgctgccgg
     2521 gacataagca aagccggcgc gcaggagttg ggccacgggg tcccaaccgg gtggcggtgg
     2581 gatgcgccgg tcccgccacg gcatcccgtc ggcaatccac aaccggcgta gttcctcatg
     2641 gctacaattg gcgtcatgat acccgaattc ggtcccacag cactcgcaga tattataatc
     2701 ggttggtggg tcttccagct tatcgtagcc acagacaggg caggtgtaat ccatcgcact
     2761 ccttcttgtg tcactctgag cgcagcgaag agtctcggcg cttgccccac gagacgagat
     2821 tcttcgctcc gctcagaatg acacgggcgg cgtagttcgc tatcccccat cgtctgtcac
     2881 tctgagcgca gcgaagagtc tcgttccggt gcgcgacgag attcttcgct ccgctcagaa
     2941 tgacaagggc gggtgtgata ggcacaaata aagggggcga gtctggcggc tcgccccctg
     3001 gcagcgataa cctgaattgg acaatggtcc tgcctgggtg gtcagcagcg atgctgtcgc
     3061 gcccttttaa agcggggatc caggttatct gcaattcgct gag
//
LOCUS       DASUYC010000835         4044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1285580, whole genome shotgun sequence.
ACCESSION   DASUYC010000835 DASUYC010000000
VERSION     DASUYC010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285580"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(236..2473)
                     /locus_tag="VF276_13685"
     CDS             complement(236..2473)
                     /locus_tag="VF276_13685"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEX6607955.1"
                     /translation="MVDLLTLFVPVAGIISVLFAVYLARDVLSRDRGTPEMQAVASTI
                     FEGAMAFLRRQYTTIAMLAVVTAVLIGILIALIPGSTLGLSSVGPGQLGVMTSIAFLL
                     GALCSGISGFIGMFVAVQANLRTASAARRSLGEALTVSLRGGAVSGFLVVSLSLLGVY
                     AIYEAYRAIGGGPSQAPFLIVGFGFGASFVALFAQLGGGIYTKAADVGADLVGKVEAG
                     IPEDDPRNPAVIADLVGDNVGDCAGRGADLFESTAAENIGAMILGVLLYAITGGTTVA
                     WILFPLVVRAFGLIASLVGLLSARVSGSGGADVTDVAALRALNTGYYVVAGLSAGFMM
                     LVSYLMFPPESWLWFGLAGLVGIATSIAFVYITQYYTAGSWRPVKEIAEATKTGPATT
                     IISGIAIGFETTFLPTISIAIALGLSFWLGTLGQPHLGAGVTLDASGEAKVMGFLVNP
                     QIASGVFGTAVATMGMLMSCAYILAMDTFGPITDNAGGIAEMSHQPESTRNITDGLDA
                     VGNTTKALTKGYAVGSAALAAFLLFSAYLDKVRQINPNLQIVDGVLPVNLSKVEVFIG
                     GFLGAMLVYLFSSLAIRAVGRAAQGMIQEVRRQFKADPGIMAGTSRPNYASCVDVSAR
                     AALREMIAPGLLAVGTPVVVGLILRWESEGALLMVGTMAGILLANVLNNGGGAWDNAK
                     KFIEAGGLRDADGQVLGKRTDAHKASVVGDTVGDPFKDTAGPSLHVLIKLLATITLVL
                     APLFV"
     gene            3101..>4044
                     /locus_tag="VF276_13690"
     CDS             3101..>4044
                     /locus_tag="VF276_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607956.1"
                     /translation="MHRYSRRVLLFLILALAAGALLLSSRPASAHLPGAAPVPEAPTA
                     PLSPVLTDVAVGPAVTTPGSLLVFTYTVSVTQAQSVALGAAVRPHGEVNWVSDSPRDR
                     IVALAVGSNVVTRTYQLDNTPNQTYDALWTVNSTDFTTTYDMTSRDSVVTTTAAAPTA
                     TPTSTTPPTNTPTITLTPTQTRTPTATNTPTHPVLQDVSLDPTTAYRFGYVTLYYTVF
                     SPAAQQIALGADMAPTGTNNFEDDQANNRVVSINAGVTTVSRIFAIGARAAGSYDVRW
                     GIFDPTFTTNYDLVPRDNALTIDPQSATNTPTVTNTPTA"
BASE COUNT          765 a         1420 c         1299 g          560 t
ORIGIN      
        1 cgtctggccg cccaacgacg aaaagagcgg ctcgcgcgtg gacctggaca gcagccggcg
       61 cgacgacgat gtgctgcgct tccgcgagca tctgcacgcc ctggagcacc agccggcccc
      121 gcccctggcg gcccccccgt cgtcggaccc cggcccgcgc taggcgccgg caggcgacga
      181 acaaaaaggc ggctccccaa gtgggagccg cctttttgtt gcagggccga ccgtactaga
      241 cgaacagcgg agccagcacc agggtaatgg tcgccagcag cttgatcagc acgtgcagcg
      301 aggggccggc ggtgtccttg aaggggtcgc ccaccgtatc gcccacgacc gacgccttgt
      361 gcgcgtcggt gcgcttgccg agcacctgcc catcggcgtc acggaggccc ccggcctcga
      421 tgaacttctt ggcgttgtcc caggcgccgc cgccgttgtt cagcacgttc gccagcagga
      481 taccggccat ggtgccgacc atcagcaacg cgccttccga ttcccagcgc agaatcaggc
      541 cgacgaccac cggggtaccc acggcgagca ggccgggcgc gatcatctcg cgcagggcgg
      601 cgcgcgcgct cacgtccacg cagctggcgt agttggggcg cgaggtgccg gccatgatgc
      661 ccggatcggc cttgaactgg cgccgcactt cctggatcat gccctgggcc gcgcggccca
      721 cggcgcggat cgcgagcgag ctaaacaggt agaccagcat ggcccccagg aagccgccga
      781 taaacacctc caccttcgag aggttcaccg gcagcacgcc gtcgacgatc tgcaggttgg
      841 ggttgatctg gcgcaccttg tccaggtagg ccgagaagag caggaaagca gccagggccg
      901 ccgagccgac ggcatagccc ttggtcagcg ccttggtcgt gttgcccacg gcgtcgaggc
      961 cgtcggtgat gttgcgcgtg ctctccggct ggtgcgacat ctcggcgata ccgcccgcgt
     1021 tgtcggtgat cgggccgaag gtgtccatcg ccaggatata ggcgcaggac atgagcatgc
     1081 ccatcgtcgc caccgccgtg ccgaacacgc cggaggcgat ctgcggattg accaggaagc
     1141 ccatgacttt ggcttccccg gaggcatcga gcgtcacgcc cgcgcccagg tgcggctggc
     1201 ccagcgtccc cagccagaac gagagtccca gcgcgatggc gatggagatc gtcggcagga
     1261 aggttgtctc gaagccgatg gcgatgccgg agatgatcgt cgtggccggg ccggtcttgg
     1321 tggcttcggc gatctctttc accgggcgcc aggaaccggc cgtgtagtac tgggtgatat
     1381 agacgaaggc gatagaggtc gcgataccga ccaacccggc caggccgaac cacagccagc
     1441 tttcgggcgg gaacatcagg tacgaaacca gcatcataaa ccccgcggac aggccggcca
     1501 cgacgtagta gccggtgttc agcgcgcgca gcgccgccac gtcggtcacg tcggccccgc
     1561 cgctgccgct gacgcgggcg ctgagcagac cgaccagcga ggcgatgagg ccgaaggcgc
     1621 gcaccaccag cgggaagagg atccaggcga cggtcgtgcc gccggtgatg gcgtacagca
     1681 gcacgccgag gatcatggcg ccgatattct cggcggccgt ggactcgaac aggtcggcgc
     1741 cgcggcccgc gcagtcgccc acgttatcgc ccaccaggtc ggcgattacg gccgggttgc
     1801 ggggatcgtc ttccgggatc ccggcttcaa ctttgcccac gaggtcggcg cccacatcgg
     1861 cggccttggt gtagatacca ccgcccaact gggcgaagag cgccacaaac gaggcgccga
     1921 agccgaagcc gacgatcagg aacggggcct gcgagggacc gccgccgatg gccctatagg
     1981 cttcatagat ggcatacaca cccagcaggg agagtgacac caccaggaag ccggacaccg
     2041 cgcccccacg caaggacacg gtcagggcct cgcccaggct gcgccgggcc gccgaagccg
     2101 tacgcaggtt ggcctgcacc gccacaaaca tgccgataaa accggagatg ccggaacaga
     2161 gggccccgag caggaaagca atcgaggtca tcacgccgag ttggcccggt ccgaccgacg
     2221 acaggcccag cgtgctaccg ggaatgagcg caatgaggat cccgatcagc accgccgtca
     2281 ccaccgccag catggcaatc gtggtgtact gccgtctgag gaacgccatc gccccttcga
     2341 agatcgtgct ggcgaccgcc tgcatttcag gcgtgccccg gtcgcggctc aacacgtcgc
     2401 gcgccaggta caccgcaaac agcaccgaaa tgatgccggc gacgggcacg aacagagtta
     2461 ataggtcaac cattcactcc tccacgtatt ggatagatag gattaacggc ggcctccgcc
     2521 ggccaggaga gggcacgcca cagcaatgta gccgcgagcc ggggcagcga tgtcaccggt
     2581 cgctcaagcg aggacccgga tccagaggag ggtatccgga acagcctcgg gcattgtaac
     2641 acatcgatac aatcatgtaa aggcgcccag ggttgaggaa cgggagagtt ttcatcacat
     2701 tggcggcgaa aacaaccacc tgtaccgggc agcgcctgga gtaggcggcg gttgcggggc
     2761 ggttcccgcc gctgccggca ccgccgctga ccaattcgta agaacaccca gcgccgggca
     2821 ggatctgaaa gatgcctgcg gcaggggaaa agcagcagat ctgtaagtat ttgcgtttcg
     2881 ccgatgagcg gcccgttaat cttgcgggcc tatactaaag cttgcaatta tcccgctgcc
     2941 ggataccgcc gggcacggac caccccggcc ccgccccagt tcgaccggcg ccccgttggt
     3001 gggcgcgcaa accccggcgc cggttccatg ctaccgcctg cgctcgtgag agtagccgtc
     3061 gccgtcacgc gcgggcacct gtgcgaaagg atcgtgactc atgcatcgtt atagccggcg
     3121 agtattgctg ttcctgattc tggccctcgc cgcgggcgcg ctgctgctga gcagccgccc
     3181 ggccagcgcc cacctgccgg gcgccgcccc ggtcccggag gccccgaccg cgccgctcag
     3241 ccccgtcctg accgatgtgg cggtcggccc ggcggtgact acgcccggca gcctgctggt
     3301 cttcacctac accgtcagcg tcacccaggc gcagtcggtc gcgctcggcg cggcggtccg
     3361 gccccacggc gaggtcaact gggtcagcga ctcgccgcgc gaccgcatcg tcgcgctggc
     3421 ggtgggcagc aacgtcgtca cgcgcaccta ccaactcgac aacacgccca atcagaccta
     3481 tgatgccctc tggacggtca acagcaccga cttcacgacc acttacgata tgacctcgcg
     3541 cgacagcgtg gtcacgacga cggcggcggc gcccaccgcc acgccgacca gcaccacgcc
     3601 gcccacgaat acgccgacga tcaccctcac gcccacccag acgcgcacgc ccacggccac
     3661 caacacgccc acgcatccgg tgctgcaaga cgtgtcgctc gacccgacca cggcgtatcg
     3721 cttcggctac gtcacgctct actacaccgt gttcagcccg gcggcccagc agatcgccct
     3781 cggcgcggac atggccccca ccgggacgaa caacttcgag gacgaccagg ccaacaaccg
     3841 ggtggtgagc atcaatgcgg gggtcaccac ggtgagccgc atcttcgcca tcggggcgcg
     3901 cgccgcgggc agctacgacg tgcgctgggg catcttcgac cccaccttca ccaccaacta
     3961 cgatctggta ccccgcgaca acgcgctgac catcgacccg caaagcgcga ccaatacccc
     4021 gacggtgacg aatacgccga cggc
//
LOCUS       DASUYC010000836         2423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1320345, whole genome shotgun sequence.
ACCESSION   DASUYC010000836 DASUYC010000000
VERSION     DASUYC010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1320345"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VF276_13695"
     CDS             <1..622
                     /locus_tag="VF276_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607957.1"
                     /translation="HMLLYGIDLQNPRLVDTFTTVRRYYNDMCRNAVAELAKRGKPLD
                     LDKNPALRGDVVKVYHLVAALVDQKYAENMTQAYAICNEVGAHYGWNLPMEEAIALGR
                     QAGGVSVIAHPGRAEPGFTAATGPVLDRMRAIGLDGVEAFHSYHTARDVAFYQTYAQQ
                     HGMLIGTGSDSHGPGQSPRMLTAWPAVECRALLERCGFTVADPAMS"
     gene            663..1385
                     /locus_tag="VF276_13700"
     CDS             663..1385
                     /locus_tag="VF276_13700"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX6607958.1"
                     /translation="MQQQDDSNTKKLTRRLPAVLGIGLGANVATMFVLGKLTEGLLHS
                     EVKKALDDPLKDAAQDMQNKALKPVMSLFSGLGEPWTLYPFTGFMALRWLKQDRQVDA
                     ATLGLALVGSAALNEILKKLLKRPRPEFRIHQKKASGSSFPSSHITMSLATYGTLAYL
                     VNRRRKRDEEQDKEWDKEHQARRGPAWIWAPVLAVCALIGWSRIYLGVHHPTDVLGGA
                     VAGAIMVTTCAAACNCLTPEQS"
     gene            1665..2222
                     /locus_tag="VF276_13705"
     CDS             1665..2222
                     /locus_tag="VF276_13705"
                     /inference="COORDINATES: protein motif:HMM:NF016089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX6607959.1"
                     /translation="MLPDAIGRLFGRWSEPIGGTPAGLAAVLRWLAILLRFYAGGLTG
                     LVLGWIGLQNVAVLGNIGFIPYGYDKLLGGNQRFGMDAGFFQTIGVPLPELTLILIGL
                     LELFGGLALIAGLLTRLFGFLLACNMLVAMLTVGNTAEELPLFIACALLVWTGGGMFS
                     LDQLIDQRLGHTPARAGTAERPIRS"
     gene            complement(2243..>2423)
                     /locus_tag="VF276_13710"
     CDS             complement(2243..>2423)
                     /locus_tag="VF276_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015123766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEX6607960.1"
                     /translation="LDDDILAWFREQAHAAGGGNYQTLINAALREHIRQRKEPLEATL
                     RRVVREELDKRSGAA"
BASE COUNT          389 a          869 c          768 g          397 t
ORIGIN      
        1 ccacatgctg ctctacggca tcgacctgca aaaccccagg ctggtggaca cgttcactac
       61 cgtccgccgc tactacaacg atatgtgccg caacgccgtg gccgaactgg cgaagcgcgg
      121 caagccgctc gacctggaca agaacccggc cctgcgcggc gacgtggtca aggtctacca
      181 cctggtcgcc gcgctggtgg accagaaata tgccgagaac atgacccagg cctacgccat
      241 ctgcaacgag gtcggcgccc actacggctg gaacctgccc atggaggagg ccatcgccct
      301 gggccggcag gccggcggcg tcagcgtcat cgcccatccg ggccgcgccg aacccggctt
      361 caccgccgcc accggccccg tgctcgaccg gatgcgtgcc atcggcctcg acggcgtcga
      421 agccttccac tcgtatcaca cggcccgcga cgtggccttc tatcagacct atgcccagca
      481 gcacggcatg ctgatcggca ccggctcgga cagccacggc cccggccaga gtccgcgcat
      541 gctcaccgcc tggcccgccg tggagtgccg ggcgctactg gaacgctgcg gcttcaccgt
      601 cgccgatccg gcaatgtcct agtctgcgcg tttatgccgc ggccttgcag gagaccacta
      661 cgatgcagca acaagacgac agcaacacca agaagctcac ccggcgcctg cccgccgtcc
      721 tggggatcgg cctgggcgcc aacgtggcga ccatgttcgt gctgggcaaa ctgaccgagg
      781 gcctgttaca cagcgaggtg aaaaaagccc tcgacgaccc gctgaaagac gccgcccagg
      841 acatgcaaaa caaggcgctg aagccggtga tgtccctgtt cagcgggctg ggcgaaccct
      901 ggacgctcta tccattcacc ggcttcatgg ccctgcgctg gctcaagcaa gacaggcaag
      961 tcgacgcggc cactctgggg ctggcgctgg tcggcagcgc cgcgctcaac gaaatcctca
     1021 agaagctgct caagcgcccg cgtccggagt tccgcatcca ccagaaaaag gcgtccgggt
     1081 ccagcttccc gagcagccat atcaccatga gtctcgcgac ctatggcacc ctcgcctacc
     1141 tggtcaaccg gcgccgcaaa cgcgacgagg aacaggataa ggaatgggac aaggaacacc
     1201 aggcgcgccg ggggccggcg tggatctggg cgcccgtgct ggccgtctgc gcgctcatcg
     1261 gctggagccg catctacctg ggcgtgcatc atcccaccga cgtgctcggc ggggcggtgg
     1321 ccggcgccat tatggttacc acctgcgccg ccgcctgcaa ctgcctcacc ccggaacagt
     1381 cctagccctc agccgccggc ggtgtagggg ccggtttgaa accggcccgc cccgcggcca
     1441 ataaggcgct gccgcgccgc cccgcccgct actttcttcg ccggcccggc gttgtgagaa
     1501 tcaaggggct gggcgggcgg ggccgcatcc cgctcgcctc gccgccggcg ctgtgccccg
     1561 gctcttgtgc cgggggtagc cgcgtgctag aatggatgcg atgacatgcg gccggtggtc
     1621 cgcgggcgct gtctcgcgtc ctgcggttat caggaggata tccgatgcta cctgatgcga
     1681 ttggtcggtt gttcggacgc tggtcggaac ccatcggtgg cacgcctgcc gggctggccg
     1741 cggtgctgcg ctggctcgcc attctactgc gcttctacgc cggggggctg accggcctgg
     1801 tgctcggctg gatcgggctg caaaatgtgg ccgtcctggg caatatcggc ttcatcccct
     1861 acggctacga taaactgctg ggcggcaacc aacgcttcgg catggacgcc gggttcttcc
     1921 agaccatcgg cgtacccttg cccgaactga ccctgatcct catcggcctg ctcgaactgt
     1981 tcggcggcct ggcgctgatc gccggcctgc tgacgcgcct gttcggcttc ctgctcgcct
     2041 gcaacatgct cgtcgccatg ctgacggtcg gcaacaccgc cgaggagctg ccgctcttca
     2101 tcgcctgcgc gctgctcgtc tggacgggcg gcggcatgtt ctccctcgac cagctcatcg
     2161 accagcgcct gggccacact cccgcccgcg cgggcacggc ggagcgacct atccgcagct
     2221 aggccgtccc gcgaggcgcg ggttaggccg cgcccgatcg cttgtccagt tcttcgcgta
     2281 ccacgcggcg caaggtggcc tccagcggct ccttgcgctg tcggatatgc tcccgcaacg
     2341 cggcgttgat cagggtctgg tagttcccgc ctcccgccgc atgggcttgc tcgcggaacc
     2401 acgccaggat gtcgtcgtca agg
//
LOCUS       DASUYC010000837         4769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_500985,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000837 DASUYC010000000
VERSION     DASUYC010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500985"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..537)
                     /locus_tag="VF276_13715"
     CDS             complement(103..537)
                     /locus_tag="VF276_13715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607961.1"
                     /translation="MDDKQYLRQLLAEMESADVLTIFFPLLRKALVVDTRRSSTHRPT
                     AGVVPAVSSISERVALIQRERPELGPVQAILSIPWIKSVESFREGEVFERTIACLARA
                     GVPEADAREICLAAAGELRAAEYAAKVALVRGENCDTVWPVL"
     gene            complement(990..1127)
                     /locus_tag="VF276_13720"
                     /pseudo
     CDS             complement(990..1127)
                     /locus_tag="VF276_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963798.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
     gene            complement(1291..2763)
                     /locus_tag="VF276_13725"
     CDS             complement(1291..2763)
                     /locus_tag="VF276_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607962.1"
                     /translation="MDQPDDKRSGEPYTYLPQGGDATGPQTPEHVTLGGTDAGPAPAV
                     NPSVGTPGTPAPPVPPPVIHRAGDAHPGESNPAAAYTRPDGKPVDTAQLLAAREAAEE
                     AISRGEGQAVPYEDEGDDLALAREDQGGRNMAPDATPTPADYLAAPHPYGPLASAGAG
                     LEEDTVRMEVKQEANAAVVQQAESMSGADPERIDYERREFGQSQMQELRDDVILGAMT
                     GPTPPPEPARDVTAVFASVDQARAAIEALREIGIPADSISLVARDLGSRGEAVATVDE
                     VTPAGEGSFRRSNTALPNDEDLPTTVMAQTPEAPATVPRGGLARDEGEITRIDSPADP
                     DIYSDFAESDADYRTTAGEASAQTGEPVAGIDVNVDRADTEAGDPGHVDAGRGLVAGG
                     IFGGLTGLLVGLGALAIPGLGPIIAAGPLAGAITGILAGGATGGIAGALLDAGVPDEH
                     ATALAGRVAGGDVLVSVQTDQLTHDAVVRTLQTQGAEEVH"
     gene            complement(3023..>4769)
                     /locus_tag="VF276_13730"
     CDS             complement(3023..>4769)
                     /locus_tag="VF276_13730"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX6607963.1"
                     /translation="DTVPLPSAPAPVPAGLTPQDAPTEILPFDLDVLHPLPDGAILAG
                     RYVVRGLIQHAANLNLYRAQARNAQRCPNCGALAAPDAQICARCQAALTGAAPLPAYV
                     VAEARDRDALLRDPSVIERGLQHPNLMPLVDEFSYAPYGPARYYTAAEARQGVPLAQL
                     PPPQPAAQVVEWGLQLTEALRYLHEQGVLSPGATAGNVLIKDNEAVLANLQQARPASP
                     DPRERGREFAADVTALAGLLYETLTGHEAPAAGGGPLLLDSAPPALETAFSRALRPGP
                     DSPPLTAEGWHALLLQAREALHAPAPSLRIRSGRISDVGRHRPLNEDSLGVIECQVVQ
                     ESVSAGVGVYAVADGMGGHAGGEVASALAIATVTDGLVRRFIAPQFNPSSAEPTGEQI
                     TGWLTDAVQAANSRIHLERTQRGTDMGTTLVVALLTPQKLYIANVGDSRAYLWTPGDD
                     PAAGLRQVSVDHSLVQRLVTMGQITPEEAKYHPYRNMIYKSLGEKSQVEIDLFIEPVA
                     PGLRLLLCSDGLSGMVPDPQIAEILAAEADPQAACARLIAAANEGGGTDNITAVTVYV
                     EASGDARNTDAPGAI"
BASE COUNT          611 a         1619 c         1786 g          753 t
ORIGIN      
        1 tgagcgacca ggccggtttg aaaccgaccc ctacagaggc caaccggtgc gcccggcagg
       61 ccccgatacc tgctatggcg gcggatgccc gttagccctg gctcagagga ccggccagac
      121 ggtgtcgcag ttctcgccgc gtacgagggc caccttcgcg gcgtactcgg cggcgcggag
      181 ttcgccggct gcggccaggc agatctcgcg ggcgtcggcc tccggcaccc cggcgcgggc
      241 caggcaggca atggtgcgct cgaacacttc cccttcgcgg aacgactcta ccgacttgat
      301 ccaggggatg ctgaggatgg cctgcaccgg gccgagttcg gggcgctcgc gctgaatgag
      361 cgccacccgc tcgctgatcg aggagacggc gggcaccacc cctgccgtgg ggcggtgggt
      421 gctgctgcgc cgcgtatcga ccaccagcgc cttgcgcaac agcgggaaga agatcgtgag
      481 cacatccgcc gattccatct cggccagcag ttggcggaga tactgcttgt cgtccatgat
      541 cgctcctctg tgtggctatg aggggtatcc gggaactact gtcgcgggca ttataccata
      601 acgccgcaag gcccgacgcc tggcgggtgc cgggggtgcg cggttagaga acggggtgtt
      661 tcggatcggg cacagaggcc gggccgggga agccggtcac gctaattccg cggtgtaggg
      721 gccggtttga aaccggcctg ccgcccatca gaatcacggt ggggggttag gtgggttgat
      781 ccgccggccg cgcgccggcg ggaagcgggg ccggatcggg gccgggcaca gcactactgc
      841 ccgaccccgg agtccggggt aacttacttg ttcttgctgc tgcttttgct gccgccgctg
      901 gatgcgtgcg atcccccgcc gccgctggac gccttcgcgc ccccgccgcc gctggagctg
      961 ccgccccccg cggaggaccc ggatcccgac ccgatgcgga acatcgaggt gccacagacc
     1021 gggcaggtgc ccttggtggc gggccgtcca ttctgcatgg taacttcctg cgggtccttc
     1081 atctcacgtt tggtgcggca cttgacacaa tatgcttcag ttgccatttg gggaattcct
     1141 cctatcgtaa aacctgcaac ccgccctccc aagcgagaac ggggccgggg cgcgccgcgt
     1201 ggaccggggt cgcacgcccg caccctgcta ggccggcgcc accgtcaccg gtgccgggcg
     1261 gcgaatagaa tccggcgggg cggaagccgg ttaatggact tcctccgcgc cctgggtttg
     1321 cagcgtccgc accaccgcgt catgcgtgag ctggtcggtc tgcacggaca ctagcacgtc
     1381 cccccccgcg acccggccgg ccaacgctgt cgcgtgctcg tcgggcaccc cggcgtcgag
     1441 cagggcgccg gcgatgccgc cggtggcgcc gcccgccagg atgccggtaa tggcgccggc
     1501 cagcggcccg gcggcgataa tcgggcccag gccggggatc gccagcgcgc ccaggcccac
     1561 cagcagcccg gtgaggccgc cgaagatgcc cccggcgacg aggccgcgcc cggcatcgac
     1621 gtggccgggg tctccggcct cggtgtccgc ccggtccacg tttacgtcga tgccggccac
     1681 cggctcgccc gtctgcgccg acgcctcgcc ggcggtcgtg cggtagtcgg cgtcggactc
     1741 ggcgaaatcg ctgtagatat cgggatcggc gggactgtcg atgcgggtga tctcgccttc
     1801 gtcgcgggcc aggccgccgc gcgggaccgt ggcgggcgct tcgggcgtct gggccatcac
     1861 cgtggtgggc aggtcctcgt cgttgggcag cgccgtattc gagcggcgga aactgccttc
     1921 gcccgccggg gtcacttcgt ccaccgtggc gacggcctcg ccccggctgc ccaggtcgcg
     1981 ggccaccagc gagatgctgt ccgccgggat gccgatctcg cgcagggcct caatcgcagc
     2041 gcgcgcctga tcgacgctgg cgaagacggc ggtcacatcg cgcgccggtt cgggcggcgg
     2101 ggtcggcccg gtcatcgcgc cgagaatcac gtcgtcgcgc aactcctgca tctggctttg
     2161 cccgaactcg cggcgctcgt aatcgatccg ttccgggtcg gcgcccgaca tgctctcggc
     2221 ctgctgaacc acggcggcgt tggcttcttg ctttacctcc atgcggacgg tgtcttcttc
     2281 cagcccggcc ccggcggatg ccagcgggcc atagggatgg ggcgcggcca ggtagtcggc
     2341 gggggtgggg gtggcgtccg gcgccatgtt gcggcccccc tggtcctcgc gggccagagc
     2401 caggtcatcg ccttcgtctt catagggcac cgcctggcct tcgccccggc tgatcgcctc
     2461 ctcggcggct tcgcgggcgg ccaggagctg ggcggtatcg accggcttgc cgtcgggccg
     2521 cgtgtaggcc gcggcggggt tggactcgcc gggatgcgcg tcgcccgcgc ggtggattac
     2581 cgggggcggg acgggggggg ccggggtgcc gggcgtgccg accgacggat tgacggcggg
     2641 ggccgggccg gcgtcggtgc cgcccagggt cacatgctcg ggcgtttgcg ggccggtggc
     2701 gtcgccacct tgtggcaaat aggtataagg ttcgccggag cgcttatcgt caggctgatc
     2761 catatcgctc tttctgcggc ccgccggggg cgcgtttgct gtccaactca cgggtggccc
     2821 gtgcctgcca ggcacactca ggcgcacttc gtgaatacaa acgcgagcca tccggctttg
     2881 ttatgtgtgc gtgcgtggcg cagcccaatt tcgtgcctga acaacctaac cccctggccc
     2941 ccttcccgcg ccgggaaggg ggaaacggga ctcccctccc cgcggcgggg aggggccggg
     3001 ggagaggttg ccggcctgcc tgttagattg cgccgggcgc gtccgtgtta cgcgcgtcac
     3061 cgctggcttc gacgtagacg gtcacggcgg tgatgttgtc tgtgcctccg ccttcgttgg
     3121 ccgccgcgat gagccgggcg caggccgcct ggggatcggc ttcggcggcg aggatctcgg
     3181 cgatctgcgg gtcgggcacc atgccgctca ggccgtcgga gcagaggagc aggcgcagac
     3241 cgggcgccac cggctcgatg aacaggtcga tctccacctg ggacttctcg ccgagcgact
     3301 tgtagatcat gttgcggtag gggtggtact tggcttcttc gggcgtgatc tggcccatcg
     3361 tcaccagccg ctggaccagc gaatgatcca cgctgacctg gcgcaaaccg gcggcagggt
     3421 cgtcgccggg cgtccagagg taggcgcggc tgtcgccgac gttggcgata tagagctttt
     3481 gcggcgtcag cagggccacg accagcgtgg tgcccatatc ggtgccgcgc tgggtgcgtt
     3541 ccaggtggat gcggctgttg gccgcctgca cggcgtcggt gagccagccg gtgatctgct
     3601 cacccgtcgg ctcggcgctg ctggggttga actgcggggc gatgaagcgg cgcaccagcc
     3661 cgtccgtgac cgtggcaatc gccagggcgc tggcgacctc gccgccggca tggccgccca
     3721 tgccgtcggc cacggcgtag acgcccacgc ccgcgctgac cgactcctgc accacctggc
     3781 actcgatcac gccgaggctg tcctcgttca acgggcggtg gcggcccaca tccgagatgc
     3841 ggcccgagcg gatgcgcagg ctgggggcgg gcgcgtgcag cgcctcgcgg gcctgcagca
     3901 agagggcgtg ccagccctcg gcggtgagcg gtgggctgtc gggaccgggg cgcagggcgc
     3961 ggctgaaggc ggtttccagc gcgggcggcg ccgagtcgag cagcagcggc ccgccgcccg
     4021 ccgccggggc ctcgtggccg gtcagcgtct cgtacagcag cccggccaac gcggtcacgt
     4081 cggcggcaaa ctcgcggccc cgttcgcgcg ggtcggggct ggccgggcgc gcctgctgca
     4141 agttcgccag cacggcctcg ttgtccttga tcagcacgtt gcccgccgtc gcgcccggac
     4201 tgagcacacc ctgttcgtgc aggtagcgca gggcctcggt cagttgcagg ccccactcga
     4261 ccacctgagc ggcgggctgc ggcggcggca gttgggccag cggcacgccc tggcgagcct
     4321 cggcggcggt atagtagcgg gcggggccgt agggcgcgta gctgaactcg tcgaccagcg
     4381 gcatcaggtt gggatgctgc agaccgcgct cgattacgct gggatcgcgc agcagggcgt
     4441 cgcggtcgcg ggcttcggcc accacgtagg cgggcagggg ggcggcgccg gtcagcgcgg
     4501 cctggcagcg ggcgcaaatc tgcgcgtcgg gcgcggccag ggcgccgcaa ttggggcagc
     4561 gctgggcgtt gcgcgcctgg gcccggtaga ggttcaggtt cgcggcgtgc tggatcagcc
     4621 cgcgcaccac gtagcgcccg gccaggatcg cgccgtcggg cagcggatgc agcacgtcga
     4681 gatcgaaggg caggatctcg gtcggtgcgt cctgcggggt gagacccgcg ggcaccgggg
     4741 cgggtgcgct gggtagcggg accgtgtcc
//
LOCUS       DASUYC010000838         5014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1980666, whole genome shotgun sequence.
ACCESSION   DASUYC010000838 DASUYC010000000
VERSION     DASUYC010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5014
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1980666"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="VF276_13735"
     CDS             complement(<1..847)
                     /locus_tag="VF276_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6607964.1"
                     /translation="MYTTTKPQTVGELRSSGYQVLPVKEEMRKNLVARLQQNEPLFPG
                     IIGYEQTVIPQIENAILSGQDIIFLGERGQAKTRIARSLINLLDDEIPVVAGCEINDN
                     PFDPTCARCREMAAELGDKLPIVWMSREARYGEKLATPDITIADLIGEIDPIKVAEGR
                     YLSDEQTIHYGLVPRTNRGIFVINELPDLAERIQVGLLNILEERDVQIRGHKVRLPLD
                     VYLVASANPEDYTNRGRIITPLKDRVGSQIRTHDPRTIEHEISIMEQERTHYKIQGVE
                     LTVPEY"
     gene            complement(950..1825)
                     /locus_tag="VF276_13740"
     CDS             complement(950..1825)
                     /locus_tag="VF276_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607965.1"
                     /translation="MQQDDYEEPFGWWGWHWQPPQPLSVVQLLEARSFDAATLGLIWA
                     LMARRASVIVGAEPPMAGKTTTLTALLDFLPPDTRRLYLHGHYETFEFASDPATEPRE
                     CYLLANEMSDHLAIYFWGARIGRLFSLLPQGYAFGSTMHAETVEEVLDILCAPPLNVP
                     PRLLPGLNLVINLVLLTQEGRYRRRCAAVHLVLPGDGPQGVSTYCLARWDAEADQLVH
                     AWTEPATLDALAQWTGTPAALLTAELAQRADYLRGLQQGEQTAIPTVRRRLAAFAQAS
                     STATPEAEHKSDIIS"
     gene            2409..3011
                     /locus_tag="VF276_13745"
     CDS             2409..3011
                     /locus_tag="VF276_13745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607966.1"
                     /translation="MILESRKWRLILRGLVLLVTTALILGFSGPASYALQDDTPHAIY
                     GRQSLSKQSLDGRFAVLRAQAELDHAVALHRDGQTMYWVPVRGYTDKEGSIKLFILTD
                     DVPSEIGTPATGDSGAVDADAFVGRLVRFAAAPYAADAEKVLITALNDPVGPDTYVLI
                     QGEAPKAYRPMVPLMGGLGVIWFLSLVSFTRAWRRSPRRR"
     gene            3186..3899
                     /locus_tag="VF276_13750"
     CDS             3186..3899
                     /locus_tag="VF276_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6607967.1"
                     /translation="MDEGGVPLTNHKTGDEDMSIAITYPSAQVGPAAAGYPAALPTAA
                     EQTAQAEERVWIDRALAGDQSAFACIVERYQTAVYNLCYRMLGSAAEAEDAAQEVFIR
                     AWSQLHTFQRDRKFSTWLLSIASHHAIDVLRRRRATVVPLDSVEIWAHSAEPQPEEST
                     IETEQRIAVRALLGTLPPKYRLVTVLRYFRDLSYLEIAEMTGLSESAVKTQLHRARKM
                     LADEIRTKGAPANVLPSIS"
     gene            3877..4614
                     /locus_tag="VF276_13755"
     CDS             3877..4614
                     /locus_tag="VF276_13755"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX6607968.1"
                     /translation="MSCRASVELMSLRLDGLLEPADITDLEQHIQTCPACTAQWTALR
                     EADRLLRAGARRPVQPPPDFAARVMTRVAATPVVRPQLWERERQQIQGGRRTVKLNSP
                     TGITGHLVPVPVGVAAAPPPRTGLAGLLDHFHPLRNARMGVYLGGISLAGVLSVLLLV
                     LTSVLSSSGLAIDASPVGSVLSQASNPTALQTWGAAVWGLVTTLLGQIDPWMITLATL
                     IVGGLAAVWWRIVAAFARRAGDREVRA"
     gene            4611..>5014
                     /locus_tag="VF276_13760"
     CDS             4611..>5014
                     /locus_tag="VF276_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX6607969.1"
                     /translation="MRSPVRRSRRHTLLLPLLAVALVLGAAVAWQQESSVAQAQPVLP
                     MPSAGEGDRIVPEGDFVLTTTQVVEHDVYVVSGDATIEEGAEVKHDVTVLSGNIVIAG
                     HVGHDVKALNGNITLAETAVVGGNVTALNGTI"
BASE COUNT          828 a         1557 c         1705 g          924 t
ORIGIN      
        1 tatactccgg caccgtcagt tccacgccct ggatcttgta gtgcgtgcgc tcctgctcca
       61 tgatcgagat ctcatgctcg atggtgcgcg ggtcgtgggt gcggatctgc gagccgacgc
      121 ggtccttgag cggggtgatg atgcgcccgc ggttggtgta gtcttcgggg ttggcgctgg
      181 cgactagata gacatcgagc ggcaggcgga ccttgtggcc gcggatctgc acgtcgcgct
      241 cttccaggat gttcaacagg cccacctgga tgcgctcggc caggtcgggc agttcgttga
      301 tgacgaagat gccccggttg gtgcgcggca ccaggccgta gtggatggtc tgctcgtccg
      361 acagatagcg gccctcggcc accttgatcg ggtcgatttc gccgatcagg tcggcgatgg
      421 tgatgtcggg cgtggcgagc ttttcgccgt aacgggcttc gcggctcatc cagacaatgg
      481 gcagtttgtc acccagttcg gcggccattt cgcgacagcg ggcgcaggtc gggtcgaacg
      541 ggttgtcgtt gatctcgcag ccggccacga cggggatttc gtcgtcaagc agattgatca
      601 ggctgcgcgc gatgcgggtc ttggcctggc cgcgctcgcc caggaagatg atgtcctggc
      661 ccgacaggat cgcgttctcg atctgcggga tgaccgtctg ctcatagccg atgatgccgg
      721 ggaagagcgg ttcgttctgc tgcaggcggg cgacgaggtt cttgcgcatc tcctctttta
      781 ccggcagcac ttggtaaccg ctgctgcgca gttcgccgac agtctggggc ttcgtggttg
      841 tatacatgct ccgtgctttc ctagtcctgc accgggcggg aacggttccc ggcgggccga
      901 tgcaatgagc ggattgtcgt gtgggctgat agtacaggaa agattacgct caggatatga
      961 tgtcggattt gtgttccgct tccggcgtgg cggtggaact cgcctgagca aaggccgcga
     1021 ggcgccgccg gaccgtgggg atcgcggtct gctccccttg ctgcaggccg cgcagatagt
     1081 cggcgcgctg cgccagttcg gcggtgagca gcgcggcggg cgtgccggtc cactgcgcca
     1141 gcgcatcaag cgtggcaggt tcggtccagg cgtgcacgag ttggtcggct tcggcgtccc
     1201 agcgcgccag acagtaggtg ctcacgccct gcggcccgtc gcccggcagc accaggtgga
     1261 ccgcggcgca ccgccggcga tagcggccct cctgagtcag cagcaccaga ttaatgacca
     1321 ggttcaggcc cggcaacagc cggggcggca cgttgagcgg cggcgcgcac aggatgtcca
     1381 gcacctcctc gacggtctcg gcgtgcatgg tcgagccgaa ggcgtagccc tgcggcagca
     1441 ggctgaacag ccggccaatg cgcgcgcccc agaagtagat cgccaggtgg tcgctcatct
     1501 cgttcgccag caagtagcac tcccgcggct ccgtcgccgg gtcgctggcg aactcaaagg
     1561 tttcgtaatg gccgtgcaga tagaggcgcc gggtgtccgg cggcaagaag tcgagcagcg
     1621 cggtcagcgt ggtcgtcttg ccggccatgg gcggctcggc gccgacgatg accgaggcgc
     1681 ggcgggccat caaggcccag atcaaaccca gggtggccgc gtcgaagctg cgggcttcga
     1741 gcaactgcac caccgagagc ggctgtggcg gctgccaatg ccacccccac cagccaaacg
     1801 gttcttcgta gtcgtcctgt tgcaagccgt gcctcctctc ccagccggtt ccggataggc
     1861 cgctgtgccc gaccacgaca cgctttcatt atacacgagc ctcgtagcga cggcaaagcg
     1921 agtggtcagt ggtcggtggt cagtgatcag tacgagaagc ggaattgtca cgtgggccgt
     1981 gggaggaacc tctgctggtc ggaaactgat cactgatcac tgaccactga ccactcgcac
     2041 ctgacggctg acggctgacg gctacaaaag gagccgggca gggaactcaa ccggggatga
     2101 gaccctgccc ggcacggata gcggggtaga ccgggcgtgg ggccgcggtc gtatgggatt
     2161 gtggggcacc tcggtcgggc gaacggctgc ttggccgggg gtgcctcgtg ttgcctaccc
     2221 gtgtatatta gcatagtgtt cagcttttgt caagatattt ctgagggttt agaaaacgat
     2281 gccgaaaaaa cggcgtgttg tcacccccaa tatcggccca aagtccgcct aatgactgag
     2341 gagcgcgggc gccccgtatg gtatgatatt tgctgggggt aggccccaga aaccagggag
     2401 gagcgggcat gattctggaa agccggaaat ggcggttgat tctgcgggga ttggtgttgc
     2461 tggtgaccac ggcgctgatc ctgggctttt ccggccccgc ctcctatgcc ctgcaagacg
     2521 acacgcccca cgcgatttac ggccggcagt cgctctcgaa gcaatcattg gacgggcgat
     2581 tcgcggtgct gcgcgcacag gccgagctgg atcacgccgt agccctgcac cgcgacgggc
     2641 agaccatgta ctgggtgccg gtgcgcggct ataccgataa agaaggctcg atcaagctct
     2701 tcattctgac tgacgacgtg ccttcggaga tcggcactcc ggccaccggc gactccggag
     2761 ccgtcgatgc ggatgccttt gtggggcgcc tggtgcgctt cgccgcggcg ccctatgccg
     2821 cggatgccga gaaagtgctg atcacggccc tgaacgatcc cgtggggccg gatacctatg
     2881 tgttaattca aggggaagcg cccaaagcct atcggccgat ggtgccgctg atgggcgggc
     2941 tgggcgtgat ctggttcctt tcgttggtct ccttcacccg cgcctggcgc cgatccccgc
     3001 gccgccgtta gccatccact ctgacgctgc gaaccgggcc ggtttgcaat cggctcccca
     3061 ccactttgcc gcttcccgcc tatggtagaa tcgattcata gggtttcgat tctcaatgat
     3121 gcgcttgctt tgtaacccca gcaagcattg ttgcgtatgg atgggtgatg aaaggaaacg
     3181 cctcgatgga tgaagggggc gtcccgttga ccaatcacaa gacaggcgat gaggatatgt
     3241 ccatagctat tacgtaccca tcagcgcaag tggggccggc ggctgccggc taccccgctg
     3301 ccttgcccac ggccgcggaa caaaccgcgc aggcggagga acgcgtctgg atcgaccgcg
     3361 ctctggcggg cgaccagtcg gcctttgcct gtatcgtcga gcgttatcag acggccgtgt
     3421 ataatctctg ttatcgtatg ctgggcagcg ccgccgaggc cgaggacgct gcccaggaag
     3481 tgtttatccg ggcctggagc cagttgcata ccttccagcg cgaccgcaag ttctcgacct
     3541 ggctgctgtc gattgcctcg catcacgcga tcgacgtgct gcgccggcgc cgggccaccg
     3601 tagtgccgct ggatagcgtc gaaatctggg cccacagtgc cgaaccgcag cccgaggaaa
     3661 gcacgatcga gaccgaacaa cggatcgccg tgcgggcgct cctcgggacc ctgccgccga
     3721 aataccggct ggtgacggtg ctgcgctact tccgcgacct ctcgtacctg gagatcgccg
     3781 aaatgaccgg cctatcggaa agtgctgtga agactcaact ccatcgcgcg cgcaagatgc
     3841 tcgccgatga aattcgtacg aaaggagccc ccgcgaatgt cctgccgagc atcagttgaa
     3901 ctgatgtcgc tgcggctcga cgggttgctc gagccggcgg acatcaccga tctggagcag
     3961 catatccaga cttgccctgc ctgcaccgcg cagtggactg ccctgcgcga agccgaccgt
     4021 ttgctgcggg ccggggcccg ccgcccggtg cagccgccgc ccgattttgc ggcccgcgtg
     4081 atgacccgag tcgccgccac gccggtagtg cgcccccaac tctgggagcg cgaacgccag
     4141 cagatccagg gcgggcggcg caccgtgaaa ttgaatagcc ccaccggcat caccggccac
     4201 ctggtcccgg tgcccgtggg cgtagccgcc gcgccgccgc cgcgcacggg cctggcgggc
     4261 ttgctggacc atttccaccc gttgcgcaac gcgcgcatgg gcgtgtacct gggcggcatc
     4321 agcctggccg gcgtgctctc ggtgctgctg ctggtgctga ccagtgtgct gagcagtagc
     4381 ggcctggcga tcgacgcctc gccggtcggc agcgtgctgt cccaggcgag caaccccacg
     4441 gcgctgcaaa cgtggggggc cgccgtctgg gggctggtca cgaccctgct gggccagatc
     4501 gatccgtgga tgatcaccct ggcgacgctg attgtgggcg ggttggccgc cgtctggtgg
     4561 cgcatcgtcg cggcgtttgc gcgccgggcc ggggatcggg aggtaagggc atgagatcgc
     4621 ctgtgcggcg ctcgcgacgc catacgctct tgttgccgtt gctggccgtg gcgctcgtcc
     4681 tgggcgcggc ggtagcctgg caacaggaga gcagcgtcgc ccaggcgcag ccggtattgc
     4741 ccatgccatc cgccggggag ggcgaccgta tcgtgcccga gggcgacttc gtcctcacca
     4801 cgacccaggt ggtcgagcat gacgtttacg tcgtgtcggg cgatgccacg atcgaggaag
     4861 gcgccgaggt taagcacgac gtgacggtgc tctccggcaa catcgtcatc gccgggcacg
     4921 tcgggcacga cgtgaaggcg ctcaacggca acatcaccct ggccgagacc gccgtggtgg
     4981 gtggcaatgt gacagccctc aacggcacca tcga
//
LOCUS       DASUYC010000839         2194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_657430,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000839 DASUYC010000000
VERSION     DASUYC010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657430"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..644
                     /locus_tag="VF276_13765"
     CDS             33..644
                     /locus_tag="VF276_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX6607970.1"
                     /translation="MDSTARKEQIFQTAGSMFSRQGYHATTMRDIARELNMQGGSLYA
                     HIDSKEDMLWEIVERAAGQFLAAVQPLAALPEPASARLWRMLRAHVDVVTRNLDNATV
                     FFHEWRFLSEPRRAQIAAQRDEYEALFRRVIQDGINAGEFPHADARMGALLVLSAGNW
                     LYQWFRPDGALSAEAVAAQMADVLIAGLHCVRPGADHSPDPGC"
     gene            683..1600
                     /gene="paaA"
                     /locus_tag="VF276_13770"
     CDS             683..1600
                     /gene="paaA"
                     /locus_tag="VF276_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008336829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HEX6607971.1"
                     /translation="MEDTEQRVAEFTAYVQNGGKVEAGDWMPDEYRTQVLRFVEMHAN
                     SELMGVLPEREWINRAPNLKRKLALTAKVQDEVGHAELLYRVAEDLGKSREAMLADLL
                     AGKTKFHNVFHYPTYSWGDVAVIAWLVDAAAIQSQKALLDCSYAPYARTMTRICWEES
                     FHIKHGYDCVVTLASGTEQQRLILQDALNRWWTPLVHFHGPPTDPAKDRDLYWRIKAK
                     PNGMLRNEFLDRYVPKILELGLTIPDPSLHFNEESGHWEYDEPDWSQLKAVVTGHGPR
                     SAERLTLRRMSYADTEWVRDVVLAHRTAA"
     gene            1665..2102
                     /locus_tag="VF276_13775"
     CDS             1665..2102
                     /locus_tag="VF276_13775"
                     /inference="COORDINATES: protein motif:HMM:NF018002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607972.1"
                     /translation="MADTEMRTVAQDARPVEVRRPAGGSGATWPIFEVFRQERPGQPL
                     QHAGNLTAPDPDMAELYAREFYGRRNESIRIWVVPRAEVREIVDVDLLHPPAVDRSYR
                     SVQGYTVRDKLDQARAHGDENIPGSPGRKPRAPRNVMEIPADV"
BASE COUNT          377 a          742 c          751 g          324 t
ORIGIN      
        1 aacaagcgtt agttttctgg agcggcagga tcatggacag tacggcgcgc aaggaacaga
       61 tcttccagac ggcaggctcg atgttcagcc ggcagggcta ccatgccacg accatgcggg
      121 acattgcccg cgagttgaac atgcagggcg gcagcctcta cgcgcacatc gactcgaaag
      181 aagatatgct ctgggagatc gtcgagcggg cggcggggca gtttctggcg gcggtgcagc
      241 cgctggcggc gctgcccgag ccggcgagcg cgcgcttgtg gcgcatgttg cgcgcccacg
      301 tggacgtggt gacgcgcaac ctggataacg ccaccgtgtt cttccacgag tggcgcttcc
      361 tgagtgagcc gcgccgcgcc cagatcgccg cccaacgcga cgagtacgaa gcgctgttcc
      421 gccgggtgat ccaggacggc atcaacgccg gggagttccc gcacgccgac gcccgcatgg
      481 gcgcactgct ggtgctgtcg gcgggcaact ggctctacca gtggttccgc cccgatggtg
      541 ccctttccgc cgaggctgtc gccgcgcaga tggccgatgt gctgatcgcc gggctgcatt
      601 gcgtgcgccc cggcgccgat cactcgcccg atccgggctg ctgaagcggg cagccccctg
      661 aagccggtta gaggaggttt ctatggaaga taccgaacaa cgcgtggccg agtttaccgc
      721 ctatgtgcag aacggcggca aggtggaagc gggcgactgg atgcccgacg agtaccgcac
      781 gcaggtgctg cgctttgtcg agatgcatgc caattccgag ttgatgggcg tgctgccgga
      841 gcgcgagtgg atcaaccgcg cccccaacct caagcgcaag ctggcgctga cggccaaggt
      901 gcaggacgag gtgggccacg ccgaactgct ctaccgggtg gccgaggacc tgggcaagtc
      961 gcgcgaggcg atgctggccg acctgctggc gggcaagacc aagttccaca acgtcttcca
     1021 ctacccgacc tactcctggg gcgatgtggc ggtcatcgcc tggctggtgg acgcggcggc
     1081 gatccagtcg cagaaggcac tgctcgattg cagctacgcg ccctacgccc gtaccatgac
     1141 ccgcatctgc tgggaagagt cgttccacat caagcacggc tacgactgcg tggtcacgct
     1201 ggcgagcggc accgagcagc agcgcctcat cttgcaggac gcgctgaacc gctggtggac
     1261 gccgctggtc cacttccacg gcccgcccac cgacccggcc aaggaccgcg acctctactg
     1321 gcgcatcaag gccaagccga acggcatgct gcgtaacgag ttcctcgacc gctacgtgcc
     1381 aaagatcctg gaattggggc tgaccatccc cgacccgtcg ctgcacttca acgaagagag
     1441 cggccactgg gagtatgacg agccggactg gagccaattg aaagccgtcg tcaccgggca
     1501 cggcccgcgt tccgccgagc ggctgacgct gcgccgcatg tcctacgccg acaccgagtg
     1561 ggtgcgcgac gtggtgctgg cccaccgcac ggcggcctga ttgtcgcgac gtattgccgg
     1621 ggcgcgggat cgctgcgccc cggcgccatg cctgggggag agcaatggct gacacggaaa
     1681 tgcgcacggt ggcgcaggac gcgcgccccg tcgaggtgcg ccgcccggcg ggcgggtcgg
     1741 gcgcgacctg gccgatcttc gaggtcttcc ggcaggagcg gcccggacag ccgctgcaac
     1801 acgcgggcaa cctgaccgcg cccgacccgg atatggccga actctacgcc cgcgagttct
     1861 atgggcggcg caacgagagc atccgcatct gggtcgtgcc ccgcgccgag gtgcgcgaga
     1921 tcgtggacgt ggacctgctg cacccgcccg cggtggaccg ctcgtaccgc agcgtgcagg
     1981 gctatacggt gcgcgacaaa ctcgaccagg cgcgcgccca cggcgacgag aacattcccg
     2041 gctcgccggg tcgcaagcca cgcgccccgc gcaatgtcat ggagattccc gccgatgtct
     2101 gatccgacca agccgatgga cgccgcccgc gaggtgccgc gcttttccgc cgtgaccacg
     2161 gccaccgacg acgcgctggc cgaagccgcg cgcc
//
LOCUS       DASUYC010000840         2111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1595659, whole genome shotgun sequence.
ACCESSION   DASUYC010000840 DASUYC010000000
VERSION     DASUYC010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595659"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            316..1191
                     /locus_tag="VF276_13780"
     CDS             316..1191
                     /locus_tag="VF276_13780"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEX6607973.1"
                     /translation="MSMQARPVKIVTDSAADLPPALIRDFDISVIPLLIMIEGQEYRE
                     GVDLNNEELYQRAAKARGGASTAFPAPEVFAQAYRRLTGAGYDVVSVHLAAVLSGIVG
                     AATFATRSEGVPPDRVRVVDSRTGTLAQGWVVLAAAEAAARGLGLAEVAAAAEDAAGR
                     SFLYGGVATLEYLIRKGRIGLIPGTVGTLLNIKPIVSVRPTGEVFVLERVRTEHKLLE
                     RLGTLTAAAGPLERVGVIHAADPQAAQTLLALLEPLPVPRPIPVTPMGAVLGTSVGPG
                     AVGVCGLKRQGSAPG"
     gene            complement(1209..1973)
                     /locus_tag="VF276_13785"
     CDS             complement(1209..1973)
                     /locus_tag="VF276_13785"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX6607974.1"
                     /translation="MGRLFAVLAGCDYAGSRLDLPGLRGAEADARQFAALLAAADLPE
                     HTLAEVVVLLGARATRARIRAALRACLAAQAADDTLLCYFAGHGAQQADGLVLYAYDA
                     RYPATRLLADCGQDPAPLAFVLDTCHAGAIALSQSTARARLMTEFRLGQVQFLCSAAA
                     GQTAHERADHGVFTQTLLAALAVPREPSLWTLPLPLDRWCTVLPPDLLAEMDYSPDAT
                     ATVLSAARGAAVVPDIPGAATSGQQNWTAPAAGSGA"
BASE COUNT          292 a          724 c          802 g          293 t
ORIGIN      
        1 tatggctggc cgcggcgtcg cctgactcat gcaccggatg cgaaagttgc gcgatcaggc
       61 ttgcgatcat tatagcaccg ctctttccgt tttggcccga tccggataat attagagtgc
      121 cggcctgcgt gatacggcag gcggctgatt gcaccacgaa ggaacgaagc acgcgaagag
      181 tatttatata agttgtgttc tgcgtgacct tcgtttcttc gcggtgaatt cgacggcgct
      241 gagaggatgc ggcgcgggag gtaggcccgt gcgttcgccg gcgcacagca gcagtggagc
      301 cggcatgagg tagccatgag catgcaagcg cgacccgtga agatcgtcac cgacagcgcg
      361 gcggacctgc cccccgccct gatccgtgat ttcgacatca gcgtgatccc gctcctcatt
      421 atgatcgagg ggcaagagta ccgcgagggg gtggatctca ataatgagga gctctaccag
      481 cgggccgcca aggcgcgcgg cggcgccagc acggcgtttc ccgcgcccga ggttttcgcg
      541 caggcctatc gccggctcac cggcgcgggc tacgacgtgg tgagcgtgca tctcgccgcc
      601 gtgctgagcg ggatcgtcgg cgcggcgacg ttcgccacgc ggagcgaggg cgtgccgccc
      661 gaccgggtgc gcgtcgtcga ctcgcggacc ggcacgctgg cgcaggggtg ggtggtactc
      721 gcggcggcgg aagcggcggc gcgcggcctg ggcctggccg aggtggcggc ggcggcggaa
      781 gacgctgccg ggcgcagctt cctctacggc ggtgtggcga cgctggaata cctgatccgc
      841 aagggtcgca tcggcctgat ccccggcacg gtgggcacgc tgctgaacat taaaccgatt
      901 gtctcggtgc ggcccaccgg cgaggtgttc gtgctggagc gggtgcgcac ggaacacaag
      961 ctgctggagc ggttggggac gctgaccgcg gcggcaggcc ccctcgagcg cgtgggcgta
     1021 atccacgccg cggatcccca ggccgctcag accctgctgg cgctgctgga gccgctgccc
     1081 gtgccgcgtc ccatcccggt cacgcccatg ggcgcggtgc tgggcacatc ggtcggcccc
     1141 ggcgcggtgg gcgtctgcgg gctgaagcgg cagggtagcg cgccggggtg agcctggcgc
     1201 cggcggcgtc aggcgccgga tcccgccgcg ggcgcggtcc agttctgttg cccgctggtc
     1261 gccgcgccgg ggatgtcggg gacgacggcg gcgccgcggg cggcgctgag caccgtcgcg
     1321 gtggcgtcgg gcgagtagtc catctcggcc agcaggtcgg gcggcaggac ggtgcaccag
     1381 cggtcgaggg gcagcggcag cgtccatagc gacggctcgc ggggcacagc gagcgcggcc
     1441 agcagcgtct gcgtgaacac gccgtggtcg gcccgttcgt gcgccgtctg cccggcggcg
     1501 gcgctgcaca ggaactgcac ctgccccagc cggaactcgg tcatgagccg ggcgcgcgcc
     1561 gtggactggg acagggcgat ggctccggcg tggcaggtat cgagcacgaa ggcaagcggc
     1621 gcggggtcct ggccgcagtc ggcgagcagc cgcgtggcgg gatagcgggc gtcgtaggcg
     1681 tagaggacca ggccgtcggc ctgctgggcg ccgtggcccg cgaaatagca cagcagcgtg
     1741 tcgtccgccg cctgggcggc caggcaggcg cgcagggcgg cccggatgcg cgcccgcgtg
     1801 gcccgcgccc ccagcagcac caccacctcg gccagggtat gctcgggcag atcggcggcg
     1861 gccagcagcg ccgcgaactg ccgcgcatcg gcctccgcgc cgcgcagccc cggcaggtcc
     1921 agccgcgacc ccgcataatc gcacccggcc agcaccgcaa acagccggcc catcgccgtc
     1981 cctcccttcc gtgtgccccg tccgcgccga tgctaccatg ccccgccgcg cgtgtcaacg
     2041 gcccccggcg ccggtgcggg tagcaccggg gtgagggcgg caacctctcc cccccgcccc
     2101 gccccgcggc g
//
LOCUS       DASUYC010000841         3004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_922840,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000841 DASUYC010000000
VERSION     DASUYC010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922840"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..1257)
                     /locus_tag="VF276_13790"
     CDS             complement(148..1257)
                     /locus_tag="VF276_13790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015190305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX6607975.1"
                     /translation="MDLRLNPPAPAFPTPERIAIVRALPGLGDLLCSVPAWRALRHAY
                     PAAQITLIGLPWAESFAARFRRYLDDFLEFPGYPGIPERPVAARAFPGWLAAAQARDF
                     DLAIQMQGNGSYINEFTLLLGARRTAGYYLPSLYCPDADLFLPYPDSGSEISRHLRLM
                     AFLGLPAQGTHLEFPLQDDDWAALAAIPAARDLPAGGYACVHPGAKAAGRRWAPAEFA
                     RVADGLAGYGLQVVLTGSADERDLTGAVAAAMRHPAVDLAGQTGLGALAALLRGARLL
                     ISNDTGVSHLAAALRLPSVVIFTGSDPDRWAPLDRARHRVVIAGTVDDSGVPVALGGR
                     SNGATPTAALPAGDLLDAVMREAAGLLEKEPEYAA"
     gene            1602..2705
                     /locus_tag="VF276_13795"
     CDS             1602..2705
                     /locus_tag="VF276_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HEX6607976.1"
                     /translation="MEQASAPGGRRESPGPDAPGGEDGGLVAVRQEPFNAESPLAALR
                     TPITPSDLFYVRSHFPVPALDRENWRLEIEGGSGPRTLTFAGLAALPAQTLTATLECA
                     GNDRRGFAPLPPGEPWGMGAVSTGVWRGVALHRVLELAGVPAGVAEILFEGADHGPPA
                     GNDAATGFVRALPAAKAFDPDTILAYELNGAPLPPAHGGPVRLLVPEWYGVASVKWLA
                     RIAALAEPFVGFFQTERYILDIPGQTPAEPLRAMAVKSLITDAPPGAGGARGPVRVAG
                     MAWSGAGPIATVEVSVDGANWQPARLVGSAPPHAWQAWEWEWAAPAPGRHVLRVRATD
                     AAGNTQPEVAPWNRLGYVNNAIQALALEIRAED"
BASE COUNT          452 a         1047 c         1113 g          392 t
ORIGIN      
        1 acctggctgc ccgcgggcga ggccatgagc gtgatatggg ccgcgggcag cgcctggcgc
       61 aacgcgcgca gggccgggcc gagcatcacg atgtcgccga tattatcgag ccggatcacc
      121 agcaggcgcc ggacgtggtg ccagggctca ggcagcatac tccggctcct tctccagcag
      181 gccggcggct tcgcgcatga cggcgtcgag cagatcgccg gcgggcaagg ccgccgtggg
      241 agtcgcgccg ttgctgcgcc cgcccagggc cacgggcacg ccggagtcgt ccaccgtgcc
      301 cgcgatgacg acgcgatggc gcgcccggtc cagcggcgcc cagcggtcgg ggtccgaccc
      361 ggtgaaaatg accacgctgg gcaggcgcag ggccgccgcc aggtgcgaca cgccggtgtc
      421 gttggagatc agtagccggg cgccgcgcag caaggcggcc agggcgccca ggccggtctg
      481 cccggccagg tccacggcgg ggtggcgcat ggccgccgcc accgccccgg tcaggtcgcg
      541 ctcgtccgcc gatccggtga gcacgacctg gagcccatag ccggccaggc cgtcggccac
      601 gcgggcgaac tccgccggcg cccagcgccg ccccgcggcc ttcgcgcccg gatgcacgca
      661 ggcgtagccg cccgccggca ggtcgcgcgc cgccgggatg gcggccagcg cggcccaatc
      721 gtcgtcctgc agggggaatt cgaggtgcgt gccctgcgcc ggcaggccca ggaacgccat
      781 cagccgcagg tggcgggaga tctccgagcc gctgtcgggg tatggcagaa agagatcggc
      841 atcggggcag tagaggctgg gcagatagta ccccgccgtg cgccgcgccc ccagcagcag
      901 cgtgaactcg ttgatatagc tgccgttgcc ctgcatctgg atcgccaggt cgaagtcacg
      961 ggcctgggcc gccgccagcc agccgggaaa cgctcgcgcc gccactggcc gctcggggat
     1021 gccgggatag ccagggaatt ccaggaagtc gtccaggtag cgccggaacc gggccgcgaa
     1081 gctttcggcc cagggcagcc cgatcagggt gatctgcgcc gctgggtagg cgtggcgcag
     1141 ggcgcgccag gcgggcacgc tgcacagcag atcgcccagc cccggcaggg cgcggacgat
     1201 cgctatacgc tcaggagtgg gaaaagcagg tgccggcgga tttagccgca aatccatcat
     1261 cggatccatc tctgtcgcgc cgcgctcagg ccattacacg cgcgggcaca ccctgtgtac
     1321 cccgccggca cacgaagcga gcgggcagat aatcgccgga ttgtacaatc gcaagtcagg
     1381 acgcggcgac caacaaagaa tcactgcggc atttgcttct tgctgtagca actcgtgtgc
     1441 cagccccaac ggaggtggtc ccgcccgccg atcaccagcc gcgatcggcc aacggagcgc
     1501 attgcgaacc gcgcgcttcc ggtgtagact agggccagcc gtggcggccc ggcgattggc
     1561 gcggccccgc cggcggccgg cagcataagg agggcaggag catggagcag gcgagcgcgc
     1621 cgggtggacg gcgcgagagt ccagggccgg acgcacccgg cggtgaggat ggggggctgg
     1681 ttgccgtccg gcaggagccg tttaatgccg agtcgcctct ggcggcgctg cggacaccca
     1741 tcacgccaag cgatctgttc tatgtgcgca gccatttccc ggtgcccgcg ctcgaccgcg
     1801 agaactggcg gctggagatc gagggcggca gcgggccgcg cacgctgacc ttcgccggcc
     1861 tggccgcctt gcccgcgcag acactcacgg ccaccctgga gtgcgcgggc aacgaccgcc
     1921 gcggcttcgc cccgctgccg ccgggcgagc catggggcat gggcgcggtc agcacgggcg
     1981 tctggcgggg ggtggcactg caccgggtgc tggaactggc cggtgtgccc gccggcgtgg
     2041 ccgagatcct gtttgagggc gcggaccatg gcccgcccgc cggcaacgac gcggcgaccg
     2101 gcttcgtgcg cgcgctgccc gccgccaagg cctttgaccc cgatacaata ctggcctacg
     2161 aattgaacgg cgccccgctg cccccggcgc acggcgggcc ggtgcgcctc ctcgtgccgg
     2221 agtggtacgg cgtggcttcg gtcaagtggc tggcgcgcat tgccgcgctg gccgagccgt
     2281 tcgtgggctt cttccagacc gagcgctata tcttggacat tcccggccag acgcctgcgg
     2341 agccgttgcg cgccatggcc gtcaaatcgc tgatcacgga tgcgccgccg ggcgccggtg
     2401 gggcgcgggg gccggtgcgc gtcgcgggga tggcgtggtc gggcgccggg cccatcgcca
     2461 cggtcgaagt cagcgtggac ggcgcaaact ggcagccggc gcgcctggtc ggatcggcac
     2521 cgccccatgc ctggcaggcc tgggagtggg aatgggcggc gcccgcgccg ggccggcatg
     2581 tgctgcgcgt ccgcgcgacc gacgcggcag gtaacacgca gcccgaggtg gcgccctgga
     2641 accgcctcgg ctacgtcaac aacgccatcc aggcgctggc gcttgagatc cgcgccgagg
     2701 attagctacg ccgcgtattt agcaggttcc agacacctgg ggggaactac ccacccggac
     2761 gagcatcgag gacagcgcga tttatcgcgc aactacgccc aatagcgcgc tggaatctgt
     2821 agtgcagatt ccagcgaccg cttgtgtgta gccgcgcgat tagcgggtgc tgcaacaccg
     2881 gctgcccgcg gatttgcagc gcgcgaagac cctgatggaa gcgcgcgccg ccgtgccggc
     2941 ctgagcagcc tgggaacgcg cgcggggcct cagcgcggcg cgggcttcca ggccgcctcc
     3001 gcct
//
LOCUS       DASUYC010000842         3027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_277483,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000842 DASUYC010000000
VERSION     DASUYC010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277483"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VF276_13800"
     CDS             complement(<1..590)
                     /locus_tag="VF276_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6607977.1"
                     /translation="MVDVRAISERVGRNVEQVIVGKHNEVQLTLLALMCRGHVLIEDV
                     PGVGKTVLAKAIARSIGCTFKRIQFTPDLLPTDVSGVSIYNQKTGEFEYRPGPVMAQI
                     VLADEINRATPKTQSALLEAMEERQITVDGVTYTLPDPFLVLATQNPIEYEGTFPLPE
                     AQLDRFLMRIELGYPGRNDEIEMLDRQQQVHPLDTLA"
     gene            complement(713..1492)
                     /locus_tag="VF276_13805"
     CDS             complement(713..1492)
                     /locus_tag="VF276_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607978.1"
                     /translation="MSTIRSILTRLARGTLGLPGARGELPARPSLIDEADVEDALRYT
                     VRAEVAGAAPAGDAWGRLQRRLQQEAAAGAEIASFPLPARPALPAPRPWWLATEILPR
                     FSQLGIAVLLFLLIAGDVNSLDPFVVPARTPTAIEQSHENISVLLPGPARLSRLARIE
                     DGFSPTAAAPPVDHPAAAGPAPAAPAPDVAVQVPANREELAALRVGWALRQAPEVQRL
                     PVTPAEKPANPADGGSDLGNPLNSDGTPADSISRFYQVHAQ"
     gene            complement(1499..2293)
                     /locus_tag="VF276_13810"
     CDS             complement(1499..2293)
                     /locus_tag="VF276_13810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX6607979.1"
                     /translation="MGYLLHPGADDDAGARHGAIDVAPDLDPLAPRSLGEETLSMAAD
                     PVPPISDPAAPAGGAPLISAQAQADTALVRRVQGGDMVAFEELFHKYKGVIYRTALAI
                     TRDAGMAEEVLQDCFYKTYTNIHRIQPDVPLSPWLHRVAVNLSCNAIKRRRGWLEPIE
                     NIAERLFADPHHSPEHLFEQNELQGTIRDIVNELPVKHRVVVVLHYLHDLSLPEIADI
                     LECPVGTVKSRLHYARKVLKLEIESRYLTGQPAPTSSPTAVQVQKA"
     gene            2651..2735
                     /locus_tag="VF276_13815"
     tRNA            2651..2735
                     /locus_tag="VF276_13815"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2685..2687,aa:Leu,seq:gag)
     gene            2745..2818
                     /locus_tag="VF276_13820"
     tRNA            2745..2818
                     /locus_tag="VF276_13820"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2779..2781,aa:Val,seq:tac)
     gene            2845..2917
                     /locus_tag="VF276_13825"
     tRNA            2845..2917
                     /locus_tag="VF276_13825"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2878..2880,aa:Phe,seq:gaa)
BASE COUNT          560 a          958 c          989 g          520 t
ORIGIN      
        1 gccagggtgt ccagggggtg cacctgctgc tggcgatcga gcatttcgat ctcgtcgttg
       61 cggccgggat agccgagttc gatacgcatc aggaagcggt ctagctgggc ttccggcagc
      121 gggaacgtgc cttcatactc gatggggttc tgggtcgcca gcacgaggaa cgggtccggg
      181 agggtatagg tgacgccgtc gaccgtgatt tgccgctcct ccatggcctc cagcagcgcc
      241 gactgggtct tgggggtcgc ccggttgatt tcgtcggcca ggacgatctg cgccatgacc
      301 ggaccggggc ggtactcgaa ctcgccggtc ttctggttat agatcgagac gccgctgaca
      361 tcggtcggca gcaggtcagg ggtgaactgg atgcgcttga aggtacagcc gatggaccgg
      421 gcgatggcct tggcgaggac ggttttcccc acgcccggca catcttcgat caatacatgc
      481 ccgcggcaca tgagcgccag gagggttaat tggacctcgt tgtgcttgcc gacaatgacc
      541 tgctcgacat tgcgcccaac gcgctccgaa atcgcgcgca catctaccat agaagctctt
      601 ctccctcagg acggcctagc accgtcagat gtacagccat aataaagcgc ccgaccctct
      661 gggtcgggca gacgcttaca cacaaagtaa cgtacttgcc agggcgccgg atctactgag
      721 cgtgcacctg gtaaaaccgg ctgatggaat ccgccggggt gccgtcgcta ttcagcggat
      781 tgccgagatc cgacccgcca tccgccgggt tcgcgggctt ctcagccggg gttacaggta
      841 ggcgctgcac ctcgggagcc tggcgcaagg cccagcccac ccgcagggcg gccagctctt
      901 cgcgattggc gggcacctgc accgccacat ccggcgcggg cgcagccggc gccgggccgg
      961 ctgcggcggg atggtctacc gggggggcag cagcggtggg cgaaaatcca tcctcaatcc
     1021 gggccaggcg gctcaggcgg gccgggccgg gcagcaagac ggagatattc tcatggctct
     1081 gctcgatggc cgtcggcgta cgcgcgggca ccacaaacgg gtcgaggctg ttgacatcac
     1141 cggcaatgag cagaaacagc aggacggcga ttcccagctg cgaaaaccgg ggtaggatct
     1201 ccgtcgccaa ccaccagggc cgcggggcgg gcagcgcggg gcgggcgggc aacgggaacg
     1261 aggcgatttc ggccccggcg gccgcctctt gttgcaagcg gcgttgcagg cggccccacg
     1321 catcgccggc cggggccgcc ccggccacct cggcgcgtac ggtatagcgc agcgcatcct
     1381 ccacatccgc ttcgtcgata agcgaagggc gggcgggcaa ttcgccgcgg gcgccgggta
     1441 gccccaacgt gccacgcgcc aggcgagtta gtatgctgcg tatagtgctc ataacgtgtt
     1501 acgccttttg tacctgcacc gcagtggggg aggacgtggg agccggctgg cccgtcagat
     1561 agcgcgactc gatctccagc ttcagtacct tgcgggcgta gtgcaaccgg gatttgactg
     1621 tgcccaccgg gcactccaga atatcggcga tttccggcag cgatagatcg tgcaagtagt
     1681 gcaataccac cacgacccgg tgtttcaccg gcagttcgtt cacgatgtcg cggatggtcc
     1741 cctgcagctc attctgctcg aagagatgct ccggcgaatg gtgcgggtcg gcgaagaggc
     1801 gctcggcgat attttcgatc ggctccagcc agccgcgccg ccgtttgatc gcgttacaac
     1861 tcaggttgac cgccacccga tgcaaccacg gggagagagg gacgtcgggc tgaatccgat
     1921 ggatattggt gtacgtcttg tagaaacagt cttgcagcac ctcttcggcc atcccggcat
     1981 cgcgggtaat cgcgagtgct gtccgataga tcacgccctt gtatttatgg aacaactctt
     2041 caaaggccac catatcgccg ccctgtaccc gtcgcaccag cgcggtgtcc gcctgggcct
     2101 gggcggaaat aagcggggcg ccgccggcgg gggcggcagg gtccgatatt gggggcacgg
     2161 ggtcggctgc catgctcagc gtctcctccc ccagcgagcg gggggcaagc gggtccagat
     2221 ccggggcgac atcgatggcc ccgtgccgtg cgccggcgtc gtcgtcagcg ccaggatgca
     2281 acaggtagcc cattgagccc accaccgaac gcacacgcgc cggtaggtgt aggggtaagc
     2341 gtttggcgtc caagccgtct ccctatgcag tagataccac cgcccgcggc aaacggttca
     2401 atgcaacccg gcggatggta gcgcgaatca ccggcggccc ggcgcgaccc tggcgcggcc
     2461 accgcagccc ggcggcgccc cgtcccacga gccggcctgt tccggagagg tgcaaatcca
     2521 gaccgggcct gggaccgatt atagcacaag ttgcccagcg ccacaacttg acatcccggt
     2581 ccgcctgctt tataataagc acgccacccc ccaaacgcca cggcggacgg ggggatcagc
     2641 agcccggcgg gcggaggtgg cggaattggc agacgcgcta ggttgagggc ctagtggccg
     2701 ttgaggccgt atgggttcaa gtcccatccc ccgcacccga cctgggagcc ttagctcagt
     2761 cggctagagc agtcggctta catccgacag gtcacaggtt cgagccctgt aggctccact
     2821 cagcgcgccg cccaacgcgc ccacgctcag gtagctcagt tggtagagca tgcgactgaa
     2881 aatcgcaggg tcatcagttc aagtctgatc ctgagcaccc aaacataact aaacatagcg
     2941 ataaagccac gaatgacttt gtattcgtgg ctttatcgcg ctccatgtag cgacaaatgt
     3001 agccaaactt caattctgct ggcctca
//
LOCUS       DASUYC010000843         4223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_827573,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000843 DASUYC010000000
VERSION     DASUYC010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827573"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(211..1359)
                     /locus_tag="VF276_13830"
     CDS             complement(211..1359)
                     /locus_tag="VF276_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004573020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX6607980.1"
                     /translation="MGKHRLALVLLLITTFVLAGCGADAQPTATAVPASTGGSSGQGA
                     TGAKMRVAFVYVGPIGDAGWTWAHDQGRLDLMKNIPGVETAYQESVPETAADAERVIR
                     DFAQKGFNIVVTTSFGYMDPTINVAKDFPNTTFVHISGYKTAANVSTAFGKIEEPRYV
                     SGMIAGKMTKNNLMGYVAAFPTPEVIRGINAFTLGARKTNPQAKVKVVWTNTWFDPQK
                     ERQAAEALLDSGADVIAQHQDTPAPQQAAEARGKLGVGYDADMTKFAPKAVLTSPIWH
                     WGVYYTDLVKQVQAGTWKTNEYWGGWKDGIVDLGPYGQMVPADVKQMADDEAAKFKKG
                     DETIFTIFTGPLKDNTGADKVPAGQAMTDKELLSMDWLVEGVEGTVPK"
     gene            complement(1471..1884)
                     /locus_tag="VF276_13835"
     CDS             complement(1471..1884)
                     /locus_tag="VF276_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3830 family protein"
                     /protein_id="HEX6607981.1"
                     /translation="MRMLRITAGSLQFTAQMEEADAPQTCAAFSKLLPWRSKIIHARW
                     SGEAAWIPLGNFQLGVGYENHTSYPAPGQVLFYPGGFSETEILFPYGSSRFASKVGQL
                     AGNHFLTIVAGREQLRDLGRMVLWEGAQDILFEAI"
     gene            2288..3025
                     /locus_tag="VF276_13840"
     CDS             2288..3025
                     /locus_tag="VF276_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009672592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEX6607982.1"
                     /translation="MCGRYTLTATPQQIALRFGVDPAPVAADLAPRYNIAPSQANPVV
                     VNQDGPALALMQWGLIPSWSREPSSRYSTINARIETVADKPAFRGPLRSRRCLVPATG
                     YYEWQARPGAPARAPKQPYFITLGHDGGPGELFAFAGLYDIWRAPDGQELATYTILTT
                     AANDLLRPLHERMPVILPREAEAQWLDPAISDPARMLALLQPPPEDRILAYPVSPAVN
                     TPASDSADLIAPVPGIAAIQLQAPQLF"
     gene            complement(2994..3551)
                     /locus_tag="VF276_13845"
     CDS             complement(2994..3551)
                     /locus_tag="VF276_13845"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX6607983.1"
                     /translation="MPDLSFKYCPACGTALVKRLVGGVMRTACAVCDFVVFADPKVVA
                     LVVVEHAGQVLLGRRNMNPARGQWSFITGYVNRGEKVEDAAIREVKEETNLDVRLDGL
                     LGVYSATGRPHILIAYHASVTAEALAPLAPQPEEVSELAFFGPDAMPPLAFPGDYEIF
                     ADWRRIVTERPAATPPKKAAEPATG"
     gene            3760..>4223
                     /locus_tag="VF276_13850"
     CDS             3760..>4223
                     /locus_tag="VF276_13850"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX6607984.1"
                     /translation="MANTKELAAQFLQALVAKDAATVEALLGDDAALRIGSWRGSEAY
                     RPRDRVLARLQAEWAGWPDPRLEVFAVLGDETHVAVEFRIQVTEQGRYVEHNRAAFFT
                     VAGERITLIDLYCPEPMPSAHRKGWIAPATLSEAEIRQALVEMRSRFDLREW"
BASE COUNT          666 a         1393 c         1398 g          766 t
ORIGIN      
        1 gcgccgttct cgcccagcag cgcgtggacc tcgcccgcct gcgcggtgaa gttcacggca
       61 tcgttggcga gcaccccggc aaaccgtttg gtgatgccgg tcatctcgac ggcgttcatg
      121 cgcgttccta gccgttagcc gtcagccgtc agccgtcagg tgagtacgtt aggtatacct
      181 gacggctgat agctgatagc tcgtcgcgtc ctatttcggc acggtgcctt cgacaccctc
      241 gaccagccag tccatgctca gcaattcctt gtcggtcatg gcctggccgg cgggcacctt
      301 atcggcgccc gtgttgtcct tcagcgggcc ggtgaagatg gtgaagatcg tctcatcgcc
      361 cttcttgaac ttggcggcct cgtcgtcggc catctgcttc acgtcggctg ggaccatctg
      421 cccgtagggg ccgaggtcga caatgccgtc cttccagccg ccccagtact cgttcgtctt
      481 ccaggtgcca gcctggacct gcttgaccag gtcggtatag tacacacccc aatgccagat
      541 cgggctggtc agcacggcct tgggcgcgaa cttggtcatg tcggcatcgt agcccacgcc
      601 cagcttgccg cgcgcttcgg cggcctgctg cggggcgggc gtgtcctggt gctgggcgat
      661 cacgtcggcg ccgctgtcga gcagggcttc cgccgcctgg cgctccttct gcgggtcaaa
      721 ccaggtgttc gtccagacca ccttaacctt ggcctggggg ttggtcttgc gggcgcccag
      781 ggtgaaggcg ttgatgccgc ggatcacttc gggcgtgggg aaggccgcga catagcccat
      841 caggttgttt ttggtcatct ttccggcgat catgcccgag acatagcgcg gctcttcgat
      901 cttgccgaag gccgtgctga cgttggccgc cgttttgtag ccggagatat gcacgaacgt
      961 cgtgttcggg aagtccttcg ccacgttgat cgtcgggtcc atgtagccga acgacgtggt
     1021 gacgacgatg ttgaagccct tctgcgcgaa gtcgcggatc acgcgctcgg cgtcagcggc
     1081 ggtttcgggc acgctctcct ggtaagcggt ttcgacgccg gggatgttct tcatgaggtc
     1141 gagccggccc tggtcgtgtg cccaggtcca gcccgcgtcc ccgatggggc cgacgtagac
     1201 gaaggcgacg cgcatcttgg cgccggtggc gccttgcccg ctgctgccgc cggtggatgc
     1261 cggcacggca gtggcggtgg gttgagcgtc cgccccgcac ccggccagga cgaaggtcgt
     1321 gatcaggagt agaaccaatg ccaatcggtg tttgcccatc ttgtgcttta cctcccttgg
     1381 ggccgttgcc gggttgccga tggggccacg cgcccagcac caggcaggca cgcgcgacgg
     1441 ccacgcgcat gccggaagca agttgtgata ttagatggct tcaaacagga tatcttgcgc
     1501 cccttcccag aggaccatgc gccccaggtc gcgcaactgc tcgcggcctg cgacgattgt
     1561 caggaagtgg ttgcccgcca gttgcccaac cttgctggcg aagcgggaac tgccgtaggg
     1621 gaacaggatc tcggtctcgc tgaacccgcc ggggtaaaag aggacctggc cgggggccgg
     1681 gtagctggtg tgattctcgt agccgacgcc aagctggaag ttgccgaggg gaatccaggc
     1741 ggcctcgccg ctccagcgcg cgtggatgat cttgctgcgc cagggcagca acttgctgaa
     1801 cgccgcgcac gtctgcgggg cgtcggcctc ttccatctgc gcggtgaatt gcagcgatcc
     1861 cgccgtaata cggagcatgc gcaccaggtc gtgtcctcct tgctggattg tggggcgccc
     1921 ggttcccggc gcgcgccccg gatggggtaa accgtaaatc cgtccggcga cggatcgcca
     1981 ggacatgctg gcctgattat agaagggcgg caccccgttg tcaaccggct ctacgcccgg
     2041 cggacacaca aaagtgcttg tcgcagcgtc tttgtgcagc aaagtttatt ccgagtctcc
     2101 acgctgctaa tcccattagc agcaacatgg tgcgcacgat tccgtttttg cggggccgcc
     2161 ggccatataa tagtataggg ccctgagggc tagctgccgg tataccggcg agatatagat
     2221 ccgtcgcata ttctgtggcc cggcgatccg ttcgcgggtg gccgggccgc cggcaaagga
     2281 gtgacccatg tgtggacgtt acacgcttac cgctaccccg cagcagattg cgctgcgctt
     2341 cggcgtggac ccggcgcccg tcgccgctga cctggcaccg cgctataaca tcgcgcccag
     2401 ccaggccaac ccggtggtcg tcaaccagga tggcccggcg ctggcgctga tgcagtgggg
     2461 gctgatcccg tcctggtcgc gcgagccgag ttcgcgctac tcgaccatca acgcgcggat
     2521 cgagaccgtg gccgacaagc cggccttccg cgggccgctg cgcagccggc gctgcctggt
     2581 cccggccacc ggctactacg agtggcaggc ccggcccggc gcacccgccc gcgcgcccaa
     2641 gcagccttac ttcatcacgc tgggccatga cggcgggccg ggcgaactgt tcgccttcgc
     2701 cggcctctac gacatctggc gcgcgcccga cgggcaggag ctcgccacct acaccatcct
     2761 gaccacggcg gccaacgacc tgctgcgccc gctgcacgag cgcatgccgg tgatcctgcc
     2821 gcgcgaggcc gaggcccagt ggctcgaccc ggcgatcagc gacccggccc ggatgctggc
     2881 cctgctccag cccccgcccg aagaccgcat cctcgcctac ccggtctccc cggcggtgaa
     2941 caccccggcc tccgacagcg cggacctgat cgcgccggtg ccgggaatag cggctatcca
     3001 gttgcaggct ccgcagcttt tttaggcggt gtggcggcgg gtcgctccgt gacgatccgc
     3061 cgccagtccg cgaaaatctc gtaatcgccg gggaaggcca gcgggggcat cgcgtccggg
     3121 ccgaagaagg ccagttcgct gacttcctcc ggctgggggg ccaggggggc cagcgcttca
     3181 gcggtcacgc tcgcgtggta ggcgatcagg atgtgcgggc gcccggtggc gctgtagacc
     3241 cccagcaggc cgtcgagccg cacatccagg ttggtttcct ccttgacctc gcggatcgcc
     3301 gcatcctcta ccttctcgcc ccgatttaca tatcccgtaa tgaaactcca ttgcccgcgg
     3361 gcgggattca tattgcgccg ccccagcagc acctggccgg catgttctac caccaccagc
     3421 gcgactactt tgggatccgc gaagacgacg aagtcgcaga ccgcgcaggc ggtacgcatc
     3481 acgccgccaa ccaggcgctt caccagcgca gtgccgcagg cagggcagta tttgaaagac
     3541 aggtcaggca cgggcgggat tccttctcta cagggcgggc ggggccggcg ccgcattgct
     3601 tttgagagca gcatagcacg ctctcgtgat ctcggtcaag cgcagacgtg acaggcgtat
     3661 agcgcatatc ttgaaccgcc gcgccgcctc gcatcgtatt agaggcggga tgccgggaga
     3721 tgctatcatg ccgcgatccc tggaccaaag gagtaaccaa tggcgaacac aaaagaactg
     3781 gccgcgcaat tcctgcaggc gctggttgcc aaagatgcgg cgacagtgga ggcgctgctg
     3841 ggcgacgacg cggcgctacg catcggcagt tggcggggca gcgaggcgta tcggccccgc
     3901 gatcgggtgc tggcgcggct gcaagccgag tgggcgggct ggccggaccc ccgcctggag
     3961 gttttcgctg tgctcggcga tgagacgcac gtcgcggtgg agttccgcat ccaggtgacg
     4021 gagcagggcc gctatgtgga gcacaaccgc gccgccttct tcaccgtcgc cggggagcgt
     4081 atcacattga tcgatctcta ctgcccggag ccgatgccca gcgcgcaccg caagggctgg
     4141 atcgccccgg ccacgctgtc tgaggccgag atccggcagg cgctggtgga gatgcggtcc
     4201 cggttcgacc tgcgcgaatg gat
//
LOCUS       DASUYC010000844         2463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1419609, whole genome shotgun sequence.
ACCESSION   DASUYC010000844 DASUYC010000000
VERSION     DASUYC010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1419609"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..685)
                     /locus_tag="VF276_13855"
     CDS             complement(194..685)
                     /locus_tag="VF276_13855"
                     /inference="COORDINATES: protein motif:HMM:NF039927.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5946 family protein"
                     /protein_id="HEX6607985.1"
                     /translation="MNDPVSKDAAEPAVARCPECGAPWPAGRTCSDDFHQLLAWEFED
                     PRRWEVHHLTVLCYHIQHPSLYSPGGLHGATELLGQFLEQRLSTEEVRRRIRRQVDSG
                     KRSGPITARPGAEGAYAHPVAWPMTAAQVVAGGAAAYCDNVRAWAESTLAALKASGNL
                     AAP"
     gene            complement(690..1514)
                     /gene="proC"
                     /locus_tag="VF276_13860"
     CDS             complement(690..1514)
                     /gene="proC"
                     /locus_tag="VF276_13860"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEX6607986.1"
                     /translation="MDDKLAIIGGGNMAESIIAGVLGTGLLTPEAILVTNRSVGKLER
                     LQARWGVQISYDNAHAAHWGSTILLAVKPVDMRAALASLQGAIDGKLVISVAAGVGIA
                     QVQRWLNDETHPVVRVMPNTPAQVGEAMSAWVASAAVTPAQCAQVRRLLGALGREIAM
                     TEEDALDQVTAVSGSGPAYLFYLAEQLIESGRAIGLSEDDARELVLQTIWGAAKMLRQ
                     GTVAPGTLRQAVTSKGGTTEAALAEFAHGDLPGVISRGVHAAWRRGGEIRRALAVE"
     gene            complement(1863..2399)
                     /locus_tag="VF276_13865"
     CDS             complement(1863..2399)
                     /locus_tag="VF276_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6607987.1"
                     /translation="MPALPRVIDSNPRYRKGCVMVSSIMVIDNDTHILVLLGEILTMA
                     GYEVHSYDYALPDRAELLRVRPALIICDYLLDEDAPGRGLWRMIRCEPGLAGTPLLLC
                     TTSVERLAARPGWWTGIPIIAKPFDVHDLLGAVHQALASRAGPVPAPQIGIRPASRAR
                     ALRAILSPGAGVYTPIGA"
BASE COUNT          398 a          874 c          806 g          385 t
ORIGIN      
        1 gccgggccgg tgtcaagcgc gagctggggc cgtacgttgc aaatggggat cgcggcgcgg
       61 cgccgcccgg cccctcaagg ggcgggctac gtcaaccaag gccgcctgcg cggcctaacg
      121 cgatagggaa acagcccgcg gaggcgggct tcgtagcgct agcccgcccc ttgaggggcc
      181 gggcggtgcc gtatcaaggc gccgccaggt tgccggaggc tttgagggcg gccagggtcg
      241 attcggccca cgcgcgcaca ttgtcgcagt aggcagcggc cccacccgcg accacctggg
      301 cggcggtcat cggccacgcc accgggtgcg cgtaggcgcc ctccgcgcct ggccgggcag
      361 tgatggggcc gctccgcttc cccgaatcga cctgccggcg aatacgccgg cggacctcct
      421 cggtagacag ccgctgctcc aggaactggc ccagcagttc cgtggccccg tgcaggccac
      481 ccggtgaata taggctgggg tgctggatgt ggtagcacaa taccgtcagg tggtggactt
      541 cccagcgccg cgggtcctcg aactcccagg cgagcaattg atggaagtcg tccgagcagg
      601 tgcgccccgc cggccacggc gcaccacatt cggggcaacg ggcgaccgcc ggctccgccg
      661 catccttgct gaccggatcg ttcatgcgcc tactcgacgg ccagagcgcg gcggatctcg
      721 ccgccccggc gccaggccgc atgtaccccg cgcgagatca cgcccggcaa gtcgccgtgg
      781 gcgaactcgg ccagcgcggc ctcggtggtg ccgcctttcg aggtcacggc ctggcgcagg
      841 gtgcccggcg ccacggtgcc ctggcgtagc atcttggccg cgccccagat ggtttgcagc
      901 accagttcgc gggcgtcgtc ttcgctgagg ccgatggcgc gccccgactc gatcagttgc
      961 tcggccaggt agaacaggta ggccgggccg ctgccgctga ccgccgtcac ctggtcgagc
     1021 gcgtcttcct cggtcatggc gatctcgcgg cccagggcgc ccagcaggcg ccgcacctgc
     1081 gcgcactgcg ccggcgtcac cgcggcgctc gccacccagg ccgacatcgc ctcgcccacc
     1141 tgggccggcg tgttcggcat cacccgcacc accgggtgcg tctcgtcgtt cagccagcgc
     1201 tgcacctgcg cgatgcccac gcccgccgcg acgctgatca ccagtttgcc gtcgatggcg
     1261 ccttgcagcg aggccagcgc cgcgcgcata tcgaccggct tcaccgccag caggatcgtg
     1321 ctgccccagt gcgccgcgtg ggcgttatcg tagctgatct gcacacccca gcgcgcctgc
     1381 aaccgctcca gtttgcccac cgagcggttg gtcaccagga tcgcctccgg ggtcagcagg
     1441 ccggtcccca gcacccccgc gataatactc tcggccatgt tgccgccgcc gatgatggcg
     1501 agtttgtcgt ccattgtggt acctccaggc aataaaaaag ccccgtcctc caatcaggac
     1561 gaggttgggc aaccccgcgg taccacctgc attagtctgt ggcgactcgc tcagccggca
     1621 cggcgcccag gcgcgatgcc gatcctcgtg gtaacggtag gagtccggcg ctgcttagtc
     1681 accaggcgac ccccggcgtt tccgcaggca actcacgggt gggttcggca cgccccgcga
     1741 tgctagcttg caccatccta gctcgctgaa ttcgctcccg gcgtgtgtac tatccccgat
     1801 cagtgttttt gcgtggatat gtaatttgta acccatagca tatcacaaaa ccggccccca
     1861 tgtcaagcgc cgatcggcgt atagacgccg gcccccggcg acaggattgc gcgcaacgcc
     1921 cgtgcccgag acgccggccg gatgccgatc tgcggcgcgg gcaccggtcc tgcccgcgat
     1981 gccagcgcct ggtgaaccgc gcccagcaga tcgtgaacat caaacggctt ggcaatgatc
     2041 gggatgcccg tccaccagcc ggggcgcgcc gccaggcgct cgaccgacgt ggtgcaaagt
     2101 agcagcggcg tgcccgccag ccccggctcg caccggatca tccgccataa cccgcggccg
     2161 ggcgcatcct cgtccagcag atagtcgcag atgatcaaag ctggccggac ccgcaaaagc
     2221 tcggcgcgat ccggcaacgc atagtcatac gaatgcactt catagccggc catcgtcaag
     2281 atctctccca gcagcacgag gatatgtgta tcgttgtcaa tgaccatgat ggaggagacc
     2341 atgacgcagc cctttctgta gcgcggattg ctatcgataa cgcgaggcaa ggcaggcaac
     2401 gcgggatggg cgggtcgccg gtgaggcagg tatcagaatg cggttgtata gtcatgccgc
     2461 gag
//
LOCUS       DASUYC010000845         8816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_594833,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000845 DASUYC010000000
VERSION     DASUYC010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 8816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8816
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594833"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1979)
                     /gene="tyrS"
                     /locus_tag="VF276_13870"
     CDS             complement(<1..1979)
                     /gene="tyrS"
                     /locus_tag="VF276_13870"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX6607988.1"
                     /translation="MQFFLGDDLLARAPDYMVALLVARFTSPPGASAAEWAAGLLADA
                     TATLPERLAPPAPGIDPTNPVALVRADPRVAVWRGAMQGFGINPNRYPSSIEALASRV
                     VKGGQIPSVTALVDLANAVALRHVVPLGAHDVDALPADLGVRLSRAGDTFLTEGATEA
                     VPPGEPVYAAGQSVRTRRWIWRQADEGRISGQTTRLLFPIDGFHGQTDGVVLAAAQDL
                     YDLLSSLGPASLALYRLDAGCRAVELVDIPNLTAPDIRALPIAGDLRAQFARLAEAPQ
                     VTIPGTPRAPEQEQKTMTEPQTAPPPPAGEVSTDDAGIREVLERGVEIIEVRADLEKE
                     LRSGRRLRVKLGIDPTGPRLHIGRAVTLRKMRQFQRLGHQAVLIVGDMTALIGDASDK
                     DAERPMLTSEQVRENMRTYVQQIGLVVDVDKAEFQYNSTWLDALSFKDVIQLAKHFSV
                     AQMIQRENFRNRWDEGERIGLHELLYPLMQGYDSVAIKADVELGGTDQLFNVMAGRKL
                     QEVFEQKPQNILLTGMVLGLDGRKMSTSWGNGVFINDAPVDQYGKMMSASDELIVPYL
                     ESCTDVPMDEVRTIAADLHSGAAHPKEVKKFRAWQIVNLYHGADAANFAQAEFEKVFE
                     QRSAPSDIPAVTVPAVETRVVDLLLATGQAPSKGQA"
     gene            2541..2948
                     /locus_tag="VF276_13875"
     CDS             2541..2948
                     /locus_tag="VF276_13875"
                     /inference="COORDINATES: protein motif:HMM:NF027286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HEX6607989.1"
                     /translation="MGIPTHEDAKLMLQLVRMRQDPRFLENENWFLREFRPGPWSEVS
                     HRYPPGTQERNRLDSVLGYWEMVGSLIDHGLLSEDLLFDVLTSMEPVWERIEPWVIEA
                     RAAQGNDTYENIEILVDRQRRWRQLHRPKSTRL"
     gene            2967..3503
                     /locus_tag="VF276_13880"
     CDS             2967..3503
                     /locus_tag="VF276_13880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature-induced protein"
                     /protein_id="HEX6607990.1"
                     /translation="MSSEVAVGVFADRAKAAAAIQALQARGFAPADVSVIMNDPVVEA
                     EEEAGHEGAETGATLGGVLGGIGGWLIGAGVLMIPGLGPLLAAGPLMAALGGAVVGAG
                     TGGIVGVLTGVGLTEAHAQQLEQRVREGGVVITVGPTPRHEEVRALLRELGATETDFS
                     LIGDERPAPPAPPPPATE"
     gene            3564..4421
                     /locus_tag="VF276_13885"
     CDS             3564..4421
                     /locus_tag="VF276_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEX6607991.1"
                     /translation="MAQSESSESVAGTGDVKDTTGVAPAQAATLPAPAPLEVAGPPQA
                     PPVEGQPESPTTVLLIGAHPDDAEFSSAGTIAKWVAAGRHVVYVVVTSGDKGTPDKTM
                     TNARLSSTREQEQLAAAREVGVEEVVFLRYPDGLVQPDLKLRGDITRLIRRYRPYAVM
                     VHDPLTMFYNNQFINHPDHRAVGTATIDAIYPTARDPLQYHEQIAEEGLEPHKVKEIY
                     LWGSDQPNVVSDITETLDKKIAALKHHASQIGPPEELAERIRTRAAETGALAGVPYGE
                     SYRRVVMRS"
     gene            complement(4527..5336)
                     /gene="sdhB"
                     /locus_tag="VF276_13890"
     CDS             complement(4527..5336)
                     /gene="sdhB"
                     /locus_tag="VF276_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEX6607992.1"
                     /translation="MATPTTPADRAAAPTGTAQRMVELRILRQDKPAGAAGGEPRWEE
                     FSVPYRQGMNIISCLQAIQRNPVNKAGQPTQPVVWESNCLEEVCGACTMVINGKVRQA
                     CSALVDNLTQPISLEPCHTFAVVRDLIVDRSRMFETLKKIKGWVPIDGTYDLGPGPKM
                     NEADRQISYVLSTCMTCGACLEACPNVNDATPFIGSFALNQVRLFNSHPTGAMIADER
                     LEALMDEGGIGYCDNDQNCVRVCPKGIPLTDSIAEMNRQVNFYAIKSILNK"
     gene            complement(5550..7310)
                     /gene="sdhA"
                     /locus_tag="VF276_13895"
                     /pseudo
     CDS             complement(5550..7310)
                     /gene="sdhA"
                     /locus_tag="VF276_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007202419.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
     gene            complement(7323..8039)
                     /locus_tag="VF276_13900"
     CDS             complement(7323..8039)
                     /locus_tag="VF276_13900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HEX6607993.1"
                     /translation="MATVSEPAGKVAPAEVATGSSSQMRPRTWYQRNQFWLRRLHSLT
                     GVVPVGGFLVYHFWENATALQGNTQWDEMAVHARDIQLLFFIEFGLILLPLVYHALYG
                     LVIASYARVNVAAYGYARNRLFMWQRVTGIILVLYILYHVMVLRFGAFASGAASYDIM
                     TQHLSNPLIFAFYTAGILSASFHLGNGLWSFLITWGLTVGKRSQRISQWVTTAISVVM
                     SVLGMAIIIAIVASGGGFRL"
     gene            8327..>8816
                     /locus_tag="VF276_13905"
     CDS             8327..>8816
                     /locus_tag="VF276_13905"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX6607994.1"
                     /translation="MDIDQLRAFERIVREGSFSRAARALNITQPTTSARIATLERTLG
                     GPLFTRGGRRLTLTERGEQFLPYARRVLEVLDEGVEAARATQTGERGRVTLGLVQSLT
                     VGFLTEAVARFCALHPQTEVFVRAAQSNQVVEMLSDGLVKLGILSWPFYGTDMLPLLH
                     FRE"
BASE COUNT         1474 a         2940 c         2935 g         1467 t
ORIGIN      
        1 gcctggccct tgctcggcgc ctgcccggtt gccagcagca aatcgaccac gcgcgtttcg
       61 actgcgggca ccgtcaccgc cgggatgtcg ctcggcgcgc tgcgctgctc gaacaccttc
      121 tcgaactcgg cctgggcaaa gttggcggcg tcggccccgt gatacaggtt gacgatctgc
      181 caggcccgga acttcttcac ctctttgggg tgcgccgcgc cgctgtgcag atcggcggcg
      241 atggtgcgca cctcgtccat cggcacgtcg gtgcacgact ccaggtaggg cacgatcaac
      301 tcgtcgctgg ccgacatcat cttgccgtac tggtccaccg gggcgtcgtt gatgaacacg
      361 ccgttgcccc aactggtgct catcttgcgt ccgtcgaggc ccagcaccat gccggtcagc
      421 aggatgttct gcggcttctg ctcgaacact tcctgcagct tgcgcccggc catcacgttg
      481 aacaactggt cggtgccgcc cagttccacg tcggccttga tcgccaccga gtcgtagccc
      541 tgcatcagcg ggtagagcag ttcgtgcagc ccgatgcgct cgccctcgtc ccagcggttg
      601 cggaagttct cgcgctggat catctgggcc acactgaagt gcttggcaag ctggatcaca
      661 tccttgaagc taagcgcatc cagccaggtg ctgttgtact ggaactcggc cttgtctaca
      721 tccacgacca ggccgatctg ctggacgtag gtgcgcatgt tttcgcgtac ctgctcgctg
      781 gtcagcatgg gccgctcggc gtccttgtcc gaggcgtcgc cgatcagcgc ggtcatatcg
      841 cccacgatca gcacggcctg gtggcccagg cgctggaact ggcgcatctt gcgcaacgtc
      901 acggcgcgcc cgatgtggag gcgcgggccg gtggggtcga tgcccagctt gacgcgcagc
      961 cggcgcccgg accgtagttc cttctccagg tcggcgcgga cctcgataat ctcgacaccg
     1021 cgctccagca cctcgcggat gccggcgtcg tcggtgctca cctcccccgc gggcggcgga
     1081 ggcgcggttt gtggttcggt cattgtcttc tgctcctgtt cgggggcacg cggggtgccc
     1141 ggtatcgtca cttgtggcgc ttcggccagg cgcgcgaact gggcgcgcag gtcgccggcg
     1201 atgggcagcg cccggatgtc cggcgccgtc aggttcggta tatcaactag ctcaacggcg
     1261 cggcagccgg cgtcgaggcg gtagagcgcc aggctggccg gaccgaggct gctgagcaga
     1321 tcgtacagat cctgtgccgc ggccagcacc acaccgtcgg tctgcccatg aaacccgtcg
     1381 atcgggaata acagccgcgt ggtctgcccg ctgatgcggc cctcgtcggc ctgccgccag
     1441 atccagcggc gcgtgcgcac cgactggccc gcggcataga ctggctcgcc cggcggcacg
     1501 gcttcggtgg ctccttcggt caggaacgtg tcgcccgcgc ggctcagccg cacgcccagg
     1561 tcagcgggca gcgcgtccac gtcatgcgcg cccagcggca ccacgtggcg cagcgccacc
     1621 gcgttcgcca gatcgaccag ggccgtcacc gacgggatct gcccgccctt gaccacgcgg
     1681 ctcgccagcg cctcgatgct gctcggatag cggttggggt tgatgccgaa cccctgcatc
     1741 gccccgcgcc agacggccac gcgcgggtcg gcgcgcacca gggcgaccgg gttggtcggg
     1801 tctatgccgg gcgcgggcgg cgccaatcgc tccggcagcg tggccgtggc atccgccagc
     1861 aagcccgcgg cccactccgc cgccgatgct ccgggaggac tcgtgaagcg cgccaccagc
     1921 agcgccacca tatagtcggg cgcccgcgcc agcaaatcat cgcctaaaaa gaactgcatc
     1981 cttgctcctg tccagggcaa taaaaaaccc cgcccgccat tgcgcggacg agggtcggag
     2041 accggctcgt ggtaccaccg tgcttgcgtc ccggcctcgc ggcgcggaac ctcaccgcgt
     2101 cttccgacgc ggggcgcggt aacgggcgct cccgtgagca gcctaccggc ggcatctgcc
     2161 gcgttcggcc acaggctcca gagggtaatt cgccgccgga ggtcgccgcg cactcgcacc
     2221 acccgcgcgc tctctgtctg gttgccgctc cggccctact gggtctccgt cattgccttc
     2281 tcggtattcg gttgactctg ttatagcaca gcgccggagc cgctgtcaat ccgtcgcgcg
     2341 gcggagcgac aggcgggcct gcgacggaca aatggcgatt tcggcgcagg gcggcacaaa
     2401 aattgatctg cccctatccg gcagcgcatc tcagtaggcc cacaaacgcc cggcgcccca
     2461 gaccacgccg ctcggcagac caacgcccac tctgctatac tggggcgaac gaatcacaca
     2521 ggcaggaggc acacgcaagt atgggcatcc cgacccacga agacgccaag ctgatgttgc
     2581 aactggtccg catgcggcag gacccgcggt tcctggagaa tgagaactgg ttcttgcgcg
     2641 agtttcgccc cggcccctgg agcgaggtca gccaccgcta tccccccggc acccaggaac
     2701 gcaaccggct ggacagcgtg ctgggttact gggaaatggt cggttcgctg atcgatcacg
     2761 ggttgctgag cgaggatctg ctgtttgatg tgctgaccag catggagccg gtctgggaac
     2821 gcatcgaacc ctgggtgatc gaggcgcggg ctgcacaggg taacgacacc tacgagaaca
     2881 tcgagatcct ggttgatcgc cagcggcgct ggcgacaact gcaccggcct aaatcgacgc
     2941 ggctatagcg aacgaaggaa cggcgtatgt catcggaagt ggcggtgggc gtcttcgccg
     3001 atcgggcgaa agcggccgcc gcgatccagg cgctgcaggc gcggggcttc gccccggcgg
     3061 atgtcagcgt gattatgaac gacccggtgg tcgaggcgga agaggaagcc ggccatgagg
     3121 gggccgagac cggggccacg ctcggcgggg tgctgggcgg catcggcggc tggctgatcg
     3181 gggccggcgt gctgatgatt ccggggctgg ggccgctgct cgccgccggc ccgctcatgg
     3241 ccgcgctcgg cggcgccgtg gtcggggcgg gcaccggcgg catcgtcggc gtgctcaccg
     3301 gcgtggggct gaccgaggcc catgcccagc aactggaaca gcgcgtgcgc gagggcgggg
     3361 tggtcatcac ggtcggcccg acgccgcgcc atgaggaagt gcgcgccctg ctgcgcgagc
     3421 taggcgcgac cgagactgat ttcagcctga tcggcgatga acggcccgcg ccgccggccc
     3481 cgccaccccc cgcgacagaa tgagcaatcg caacccgaaa ggaggcagtg cggcccgccg
     3541 gccacggggg ccgcaaagtt ggaatggcac aatctgaaag tagcgaaagc gttgccggga
     3601 ccggcgatgt caaggatacc accggggtcg ccccggccca ggcggcgacg ttgcccgcgc
     3661 ccgccccgct ggaggtggcc ggcccgccgc aggcgccgcc cgtcgaaggc cagccggaaa
     3721 gccccaccac ggtgctgttg atcggcgcgc acccggacga cgccgagttt agctccgcgg
     3781 gcaccatcgc caaatgggtg gcggcgggcc gccatgtcgt ctacgtggtc gtgaccagcg
     3841 gcgacaaggg cacgccggac aagacgatga ccaacgcccg gctgtcgagc acccgcgagc
     3901 aggaacagct tgccgccgcg cgcgaggtgg gcgtggagga ggttgtcttc ctgcgctacc
     3961 ccgacggcct ggtccagccc gacctgaagc tgcgcggcga catcacccgc ctgatccggc
     4021 gctaccggcc ctacgcggtg atggtccacg acccgctgac gatgttctac aacaaccagt
     4081 tcatcaacca cccggaccac cgcgccgtcg gcacggccac catcgacgcc atctatccca
     4141 cggcgcgcga tcccctgcaa taccacgagc agatcgccga ggagggcctg gaaccgcaca
     4201 aggtgaagga gatctacctg tgggggtcgg atcagccgaa cgtggtcagc gacatcaccg
     4261 agacgctgga caagaagatc gcggcgctca agcaccacgc cagccagatc ggcccgccgg
     4321 aggagctggc cgagcgcatc cgcacccgcg ccgccgagac cggcgccctg gccggcgtgc
     4381 cctacggcga aagctaccgc cgcgtggtca tgcgcagcta gacagcgcgc tactggcgga
     4441 aagcgaaaac gcgctgcccc cgtgatcctg acccggttct gcgaccggtc tgtggaccgc
     4501 gggggcggcg cgttctgctg agcgagttac ttgttcagga tgctcttgat cgcgtagaaa
     4561 ttgacctggc ggttcatctc ggcgatgctg tcggtgagcg ggatgccctt ggggcagacg
     4621 cgcacacagt tctggtcgtt atcgcagtag ccgatgccgc cctcgtccat cagcgcttcc
     4681 agccgctcgt cggcgatcat ggcgccggtg gggtggctgt tgaacaggcg cacctggttg
     4741 agggcgaacg acccgatgaa cggcgtcgcg tcgttgacgt tggggcaggc ttccaggcag
     4801 gcgccgcagg tcatgcaggt cgagagcaca tacgagatct gccggtcggc ctcgttcatc
     4861 ttggggccgg ggccaaggtc ataggtgccg tcgatgggca cccagccctt gatcttcttc
     4921 agcgtctcga acatacgcga gcggtctacg atcaggtcac gcacgaccgc gaaggtgtgg
     4981 cacggctcca ggctgatcgg ctgggtcagg ttatcgacca gggcggagca ggcctggcgc
     5041 accttgccgt tgatgaccat ggtgcaggcg ccgcagacct cttccaggca gttgctctcc
     5101 cagaccacgg gctgggtggg ctgcccggct ttgttcaccg ggttgcgctg gatcgcctgc
     5161 aagcaggaga tgatgttcat gccctggcga tagggcacgc tgaactcttc ccagcgcggt
     5221 tcgccgccgg cggccccggc gggtttgtcc tggcggagaa tgcgtagttc caccatgcgt
     5281 tgggcggttc cggtcggggc ggcggcgcga tccgccggag tagttggcgt agccatgacg
     5341 attgctgtcc tcctcggtgc ctcacccggc ccgcatgggc cggggcggca cagttacacg
     5401 cgcgcttagt cgcgccccga ctcgatcttt tcgctgccgg tcgccgcagc cgccgcgccg
     5461 ttgcccgcgc ctttcgcggc gggcgccagc gtgacggtcg tgccaccgtt gcccgcgggc
     5521 gccgcggcag gcgcgggcgc gggggtcgtc tcggcttgct tggccttgcc gctgtagtcg
     5581 cgcgaccggg gcgggatgta cgaaatatcc accgactggt aactgaactc gggcgcctcg
     5641 gtcggcccgg caaagcgggc cagcgtggtt ttcagccact gagcgtcgtt gcgcttgggg
     5701 aactcgggct tgtagtgggc gccccggctc tcgtcgcggc gcagcgcgcc cagcgtgatc
     5761 acccgcgcca gcacaagcat gttccagagc tggcggctga acagcagcgg ctggttcgac
     5821 cactcgctct tatccggcac cgtgaggttg cgatagcggc ccatcaactc ctggatcttg
     5881 acatcggtct cgcgcagctt gtcgttgtag cgcaccacgg tcacgttgtt ggtcatgacc
     5941 tggcccagtt cgcggtgcag cacgtagggg ttttccttgc cgttggcccg cttgagccag
     6001 tcgttgttct cgttctgccg ctgcacctcg gcgtcgaaga cactcgccgg cacggagccg
     6061 ctgccgcgcg ccgcgtcctt cgcgtactcc accgcgctcg gccccacgac atgcaggccg
     6121 gtatagatgc aggacagcag cgagttggcg cccaggcggt tggcgccgtg gtattggtaa
     6181 tcggcctcgc cgccggcgaa caggccgggg atgttggtca tgccgtgctc gttgtccacc
     6241 cagaggccgc ccatcgagta atgcacggcg gggaagatgc gcatcggcac cttgaccggg
     6301 tcgtcgccca cgaacttgcg gtagatctcc atcacgccgc ccagcttgac ttcgagcatg
     6361 tgcgggtcga gatggctcac gtcgaggtag acctggttct gcccgtccac gcccaggccc
     6421 atatccacgc agacatggaa gatctcgcgc gtggcgatgt cgcggggcac caggttgccg
     6481 tattcgggat acttctcctc caggaaatac cagggcttgc cgtccttgta cgtccagacg
     6541 cgcccgccct cgccgcggat cgactcggac atcagccgca gcttgtcctc gccggggatc
     6601 gccgtggggt ggacctggat gaactcgccg ttggcataga gggcgccctg ctggtagacg
     6661 gcgccggcgg cggtgcccgt gttgatcatc gagttggtcg agagaccgaa gatcaggccc
     6721 ggcccgcccg ttgccaggat cacggcatcc gcggggaagg agcggatcgc catgctcttc
     6781 atgtcctggg cgacgatgcc gcggcagacg ccgtggtcgt cgatcacggc gctgagaaac
     6841 tcccagccct cgaacttacg gaccatgccc gcggcttcct cgcggcgcac ctgctcgtcc
     6901 agcgcgtaca gcaactgctg gccggtagtg gccccggcga aggccgtgcg gtggtgcttg
     6961 gtgccgccga agcggcggaa gtcgatattg ccttcgggcg tgcggttgaa catcacgccc
     7021 atgcggtcga gcaggaagat gatcgcgggg ccggtttcgc acatgccctt gaccacgccc
     7081 tggttggcga ggaagtcgcc gccgtagatc gtgtcgtcaa agtgctgcca ggcggagtcg
     7141 ccttcaccct tggtgttgac ggcggcgttg ataccgccct gcgcgcagac cgagtgcgag
     7201 cgccgcaccg gcacaatcga gaagagatcg accggtatcc ccatctcggt gattttgatc
     7261 gtcgaggaca ggccggccag cccacccccg acgacgataa tcttcggcat ctgtcgtctc
     7321 ctttacagtc tgaagccgcc cccgctggcg acaatcgcga taataatcgc catgcccagg
     7381 acactcatca ccaccgagat agctgtggtg acccattgcg agatccgttg cgaacgcttg
     7441 cccacagtca ggccccaggt aatcaagaaa gaccaaagtc cgttgcccag atggaagctc
     7501 gcgctcagga taccggccgt gtagaaagca aagatcaacg gattgctcag atgctgggtc
     7561 atgatgtcat aggaggcggc gcccgacgcg aaggccccga agcgcaacac catgacatga
     7621 tagaggatgt acagcaccag aataatgccc gtgacgcgct gccacatgaa cagccggttg
     7681 cgggcatagc cgtacgcggc gacgttgaca cgcgcatatg atgcaataac gaggccatac
     7741 aacgcatgat acacgagggg cagcaaaatc agcccgaact cgataaagaa caggagctga
     7801 atatcgcgcg catggaccgc catctcgtcc cactgggtat taccttgcag ggcggtggcg
     7861 ttttcccaga aatgatagac caaaaagccg ccgaccggca ccacgccggt caacgagtgc
     7921 agacggcgca gccagaactg gttacgctgg taccacgtgc gggggcgcat ctgtgacgac
     7981 gaccctgtcg caacttccgc cggcgcgacc ttgccggccg gctctgaaac cgttgccatt
     8041 ccatgcctcc gaagcacaag ttgatccgtg atgcggcccg gacgcgggca gtggggccgg
     8101 tcatcatggc cccggcggcg gaagcccgcg atgccgccgg gcgcctcacg actctagttc
     8161 gattgtacta caatcgctgc atcctgtcca agaaagcagg cagccacaca cagcgtacgg
     8221 cgtgaaaaac accgttcccg gacgatcata gcacccatcc ggttataaat caagacaatg
     8281 gatgctataa tatcgataga acctttttat gagacgggag cagccaatgg acatcgacca
     8341 gttacgggct ttcgagcgaa tcgtccgcga gggcagcttc agccgggcgg cccgcgccct
     8401 caacatcacc cagcccacta ccagcgcgcg gatcgccacc ctggagcgca cgctgggcgg
     8461 cccgttgttc acgcgcggcg ggcgccggct cacactgacc gagcggggcg agcagttcct
     8521 gccctacgcg cgccgcgtgc tggaggtgct ggacgagggc gtggaagcgg cacgtgccac
     8581 gcagaccggc gaacgcggtc gggtgacgct gggcctggtg cagtcactga cggtcggctt
     8641 cctgaccgag gcggtggccc gcttctgtgc gctccacccg cagaccgagg tgtttgtgcg
     8701 cgccgcgcag agcaaccagg tggtcgagat gctgagcgat gggctggtca agctgggcat
     8761 cctgagctgg cccttctacg gcacggacat gctgccgctg ctgcacttcc gcgaag
//
LOCUS       DASUYC010000846         2236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_828125,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000846 DASUYC010000000
VERSION     DASUYC010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828125"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="VF276_13910"
     CDS             complement(<1..661)
                     /locus_tag="VF276_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX6607995.1"
                     /translation="MTDEERGRAAQTAPQETEEMHVDEPAPVLVQAASGFDLPDTPDA
                     VPVGQEPLAPTELIRLPARHNPRLQRLLQKVNADEDLHAIWRCQNVTAVDRLGMSDHG
                     PVHMHIVANSALRLLRLLLARDLVPSIVKDHGLEVADAEIVVVLGALLHDTGMSIHRA
                     NHEEMSLFVALPKLQELLADLYPPAMRRVVISEVLHAIITHRSDGRPLTLEAGVVRLA
                     DA"
     gene            complement(953..1192)
                     /locus_tag="VF276_13915"
     CDS             complement(953..1192)
                     /locus_tag="VF276_13915"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6607996.1"
                     /translation="MHAYDELYTTRQAAAFLGVSELAIRTAVKEGKLRAWRLPARRDV
                     LIRKRDLLPFTDPGHELGAPRQLALFDEGDRQGAA"
     gene            complement(1262..2023)
                     /locus_tag="VF276_13920"
     CDS             complement(1262..2023)
                     /locus_tag="VF276_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607997.1"
                     /translation="MEKREKTNGSAQESLALLENPGVAPADGAETPVAVEGFDPSRYL
                     TKIDGRDYLEVKWRLLWLRTEYPTARITTEMVRYEDGFALFRAEVTLPTGAAATGWGS
                     ETIHDFADYIEAAETKAIGRALAGLGFGTQFCRDFDFAQEARADVPRAQVVDAPIGYP
                     AAVAHSETAPAGRTLVAVASNGGNRGQAEGLRATGTVTDKQLKAIYAIARAARHLSEP
                     EVDNRCRELYNCKPEELSKVEASQFIDVLKGEQAA"
BASE COUNT          342 a          723 c          778 g          393 t
ORIGIN      
        1 gtgcgtcggc caggcggacc acgcccgcct ccagcgttag aggccgcccg tcgctgcgat
       61 gggtgatgat ggcgtgcaga acctcggaga tgaccacgcg gcgcatggcg ggcgggtaga
      121 ggtcggccag caactcttgc agcttgggca gggccacgaa caggctcatc tcctcgtggt
      181 tggcgcggtg gatcgacatc ccggtgtcgt gcagcagcgc gcccagcacg accacgatct
      241 cggcgtcggc cacctccagg ccgtggtcct tcacgatgct gggcaccagg tcgcgggcca
      301 gcagcaggcg cagcaggcgc agggcgctgt tggcgacgat gtgcatatgc accggaccgt
      361 ggtcgctcat gcccagccgg tccacggcgg tcacgttctg gcagcgccag atggcgtgga
      421 ggtcttcgtc ggcgttgacc ttctgcaaca gccgttgcag ccgcgggtta tggcgcgcgg
      481 gcaggcggat tagttcggtc ggggccagcg gctcctggcc gacggggacc gcatccggcg
      541 tgtcgggcag gtcgaagccg gaggccgcct gcaccaacac gggcgccggt tcgtcgacgt
      601 gcatttcttc ggtttcctgg ggcgcggttt gcgcggcgcg gcccctttct tcgtcagtca
      661 tgaattcgtt actactcctc gtttgtcggg cgcatcgctg tcgcgcccta tacaggccgg
      721 tttgaaatca gtcccaaccc ggcccctggt tgatcggccc ataagcgcgg gccgatttca
      781 aaccggcccc tacagcggat gggcgcgcga cccaggtaca tggacggcga ttttctatac
      841 gcggctccgc gccattatag cacggcgggg ccggcaaaaa agagaacggg ttccgcgggg
      901 aacccgttcc gagatgcgtt gttcggttgg atggtcggtg gctggcgggc cgtcaggcgg
      961 cgccttgccg gtcgccctcg tcgaagaggg caagctggcg cggggcgccg agttcgtggc
     1021 cggggtcggt gaagggcagc aggtcgcgct tgcggatcaa cacgtcgcgg cgggcgggca
     1081 gccgccaggc gcgcagcttg ccttccttga ccgccgtgcg gatcgccagt tcgctcacgc
     1141 ccaggaacgc cgccgcctgg cgcgtggtgt acaactcgtc atatgcgtgc atcgcactgc
     1201 tcccttccac cgggccgggc accctggccg gggcgcccgg cgaccaccag gcaggcagcg
     1261 ttcaggcggc ctgctcgccc ttcaagacat cgatgaactg cgaggcttcg accttcgata
     1321 gctcctcggg cttgcaattg tagagctcgc ggcagcgatt atcgacctcc ggctccgaca
     1381 ggtgccgggc ggcgcgggca atcgcgtaaa tggccttcaa ctgcttgtcg gtgacggtgc
     1441 cggtggcgcg cagcccctcg gcctggccgc ggttgccgcc gttggaggcg accgccacca
     1501 gcgtgcgccc ggcgggcgcg gtctcgctgt gcgccacggc agccgggtag ccgataggcg
     1561 cgtccaccac ctgggcgcgg ggcacgtcgg cgcgggcttc ctgcgcgaag tcgaagtcgc
     1621 ggcagaactg ggtaccgaag ccgaggccgg ccagcgcgcg gccaatggcc ttggtctcgg
     1681 cggcctcgat gtaatcggcg aagtcgtgga tcgtctccga cccccagccg gtggccgccg
     1741 cgccggtggg cagggtgact tcggcgcgga acagcgcgaa gccgtcctca tagcgcacca
     1801 tctcggtggt aatgcgggcg gtggggtact cggtgcgcag ccagaggagg cgccacttca
     1861 cttcgaggta atcgcgcccg tcgatcttgg tcaggtagcg cgacgggtca aagccctcga
     1921 ctgccaccgg agtctcggcg ccgtcggcgg gcgccacgcc tggattctca agcaacgcca
     1981 gggattcctg ggcgctaccg ttggttttct ctctcttctc catctcacac gtcctccagc
     2041 tagacactca aaactcggct aagcactcaa tcttggcggt cgtagaacat ctgttctaac
     2101 atgattatac cgcacttacc atatctatgt caagccgggg ggcaaaagcg gaaggcgcgg
     2161 cgggggcaat ttcggcacgc tcttgccggg tgttcgcctg gaaatgaacc ggataaccca
     2221 tgccgggata atggat
//
LOCUS       DASUYC010000847         2007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_595302,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000847 DASUYC010000000
VERSION     DASUYC010000847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595302"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1192
                     /locus_tag="VF276_13925"
     CDS             <1..1192
                     /locus_tag="VF276_13925"
                     /inference="COORDINATES: protein motif:HMM:NF014879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyl transferase"
                     /protein_id="HEX6607998.1"
                     /translation="SGLPSAGIHVADMGSVPIPVTRYWTRISDAAGGIHLRLSPVDNR
                     VVDIRFFDSQGLELPKNTERKIETTYFREDFRRVFLDEIGRIFYPTGVTERYAEDFLQ
                     NIDVQAVHHGAPYNRIVVDYAGGSTAQILPPLLTQLGCDVVAINAAIDESSLVQTQDQ
                     FDAGMARLSAITRSVNAGFGVRLDTGGERIYLVDDTGRILEGIDALAAVAALYVTLYP
                     GSNIAVPVYAPSSLETVVERAGCKVVRTQGTPAALAQAANQPHVGLSGQGNGSFAISS
                     FHPMPDGMFAIAKLVELTARLQVKLSDVVNDLPKYAMIRKDVACPWEDKGKVMRLLNE
                     RYRDNNIPQVDGVKIMENGNEWVLILPDADQPTFHIIAEARNQDRASTMVEKYSSLVS
                     SLQG"
     gene            1426..>2007
                     /locus_tag="VF276_13930"
     CDS             1426..>2007
                     /locus_tag="VF276_13930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6607999.1"
                     /translation="MPDPTDSNIPAPQAEGAPAAPVPAPVAAAVDAVATVSPAAAQVT
                     QQAAQQATQATQQAAQVTQQAAHQVQAKIEQVGQTATVSPRRAIYQGRRFAVGYAILV
                     MMAAFLSALAKRNHTLPGDVRISRMIQRGQEDKAFDILMHAVSEMGWQWPSVITRLTF
                     STLMWAAGFRMEGAFTAATWSGDMLTMAVKETVG"
BASE COUNT          358 a          678 c          661 g          310 t
ORIGIN      
        1 ctccgggctg ccctcggcgg gcatccatgt cgccgatatg gggtcggtgc ccatcccggt
       61 gacgcgctac tggacgcgca tcagcgacgc ggcgggcggc atccacctgc gcctgtcccc
      121 ggtggacaac cgcgtcgtcg atatccgctt cttcgacagc cagggtctgg aactgccgaa
      181 gaacaccgag cgcaagatcg aaacaaccta tttccgcgaa gacttccgcc gcgtcttcct
      241 cgatgagatc gggcgcattt tctacccgac cggcgtgacc gagcgctacg ccgaggactt
      301 tttgcagaac atcgacgtgc aggcggtgca tcacggcgcc ccctataacc gcatcgtggt
      361 ggactacgcg ggcggcagca ccgcgcagat cctgccgccg ttgctcaccc aactcggctg
      421 cgacgtggtg gcgatcaacg cggccatcga cgagtcgagc ctggtgcaga cgcaggacca
      481 gttcgacgcc gggatggcgc gcctgagcgc gattacccgg tcggtgaacg ccgggttcgg
      541 cgtgcgcctc gatacgggcg gcgaacgcat ctacctggtg gacgacaccg ggcggatcct
      601 cgaagggatc gacgcgctgg ccgcagtggc cgccctctat gtgacactct accccggctc
      661 gaatatcgcc gtgccggtgt acgcgcccag cagcctggaa acggtggtcg aacgagcggg
      721 ctgcaaagtg gtgcgcacgc agggcacacc cgccgcgctg gcccaggccg ccaatcagcc
      781 gcatgtcggc ctgtctgggc aaggcaacgg ctcgtttgcc atctcctcct tccaccccat
      841 gcccgacggc atgttcgcca tcgccaaact ggtggagtta actgcccgct tgcaggtcaa
      901 gctgtcggat gtggtcaacg atctccctaa gtacgccatg atccgcaagg acgtggcctg
      961 cccctgggag gacaagggca aggtgatgcg cctgctcaac gagcgctacc gcgacaacaa
     1021 catcccgcag gtggacggcg tgaagatcat ggagaacggc aacgagtggg tgctgatctt
     1081 gcccgatgcc gaccagccga ccttccacat catcgccgaa gcccgcaacc aggatcgcgc
     1141 ctcaaccatg gtcgagaagt acagttcgct ggttagttcg ctgcaagggt agcgcgggcc
     1201 tgggtgctgt tgcggtgtag gggccggttg caaaccggcc cgccgcccgg cagaataacg
     1261 tgggggacta ctggtcgccg gccagggtgc cggtgcggta gtcccggcga tataagaaca
     1321 cggcggccgg caagacacgc cttgccggcc gttgcgatgc ccgttgcggc ggcctctggc
     1381 atagttcttg caccccgcgg cagtgatcca cagcggaggt gcttcatgcc cgatcccacg
     1441 gacagcaata taccggcacc ccaggcggaa ggcgcgcccg ccgcgcccgt ccccgcgccc
     1501 gtcgccgccg cggtcgatgc cgtggcgacg gtatcgccgg ccgccgcgca ggtcacacag
     1561 caggcggcgc agcaagccac ccaggccacg cagcaggccg cgcaggtgac gcagcaggcg
     1621 gcccaccagg tgcaggcgaa aatcgagcag gtgggccaga cggccaccgt gtcgccgcgc
     1681 cgcgctatct accaaggccg gcgcttcgcc gtgggctacg ccatcctggt gatgatggcc
     1741 gcgtttctct cggcgctcgc caagcgcaac cacacgctgc cgggcgatgt gcgcatctcg
     1801 cgcatgatcc agcgcggcca ggaagacaaa gccttcgaca tcctcatgca cgcggtcagc
     1861 gaaatgggct ggcaatggcc gagtgtgatc acccggctga ccttcagcac gctgatgtgg
     1921 gcggcggggt tccgcatgga aggcgccttc accgccgcca cctggagcgg cgatatgctg
     1981 acgatggcgg tgaaagagac ggtgggc
//
LOCUS       DASUYC010000848         7621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_133537,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000848 DASUYC010000000
VERSION     DASUYC010000848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 7621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7621
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133537"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..1220
                     /locus_tag="VF276_13935"
     CDS             150..1220
                     /locus_tag="VF276_13935"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEX6608000.1"
                     /translation="MSADLPATAAAAPKTTIRNSAERPLGVEDYHAAAAFIRRYHRIW
                     MVLHVSPDGDSLGSNMGLAHMLRQAGHDVTVLSSDALPEYFAPFLLDKAAPQVVIGAT
                     APGPPPDAWVILDTSDPIRLGNVYTANQKLLAERPVLNLDHHPTNLEYGQLNLIDSSA
                     AAVGEQIPQLLRALDLTPDATAANWLLLGIVTDTLGFRTSNTTARTMSTAAQLMERGA
                     QLYAINEAIFNTRPLSQVLLWSRALATIKTDGRTLWTTISREMLRETGAREEETEGLA
                     SFLSGVRSMKVFALLKERGDGTRVSLRSNPGTDVSAIAMRFGGGGHKQAAGCTIPSIG
                     EAAEQQLLAAVAEVLGPLESAE"
     gene            1423..2346
                     /gene="truB"
                     /locus_tag="VF276_13940"
     CDS             1423..2346
                     /gene="truB"
                     /locus_tag="VF276_13940"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX6608001.1"
                     /translation="MTGAQGTPPATGVLVIDKPLGRTSFDVVARVRRVCGQKRVGHAG
                     TLDPLATGVLPICLGTATRLVEYLADAGKEYRARLHLGAVSATYDGEGPVTPQVTDPA
                     LLPATATILAALAAFRGPQQQVPPMHSAVQQGGKRLYELARSGVEVERRPRAVTIYRL
                     ELVAYTPPELVLDVACSKGTYIRSLAHDLGAALGCGAYLAGLVRTRHGPFGLADAVTL
                     DQLEHAVAAGRLHEVLRPPDVLVADWPRVAVAADAARRLAQGQALDYPPPAGNAPRRL
                     RVYDPDGAFLALVLWEDDKARWHPAKVFSGV"
     gene            2370..3305
                     /locus_tag="VF276_13945"
     CDS             2370..3305
                     /locus_tag="VF276_13945"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEX6608002.1"
                     /translation="MQVITDLEHFHSAPAVLTIGNFDGVHRGHQQLLGLVRDRARDLG
                     VRSTVLTFDPHTRAVVRPDQPLQLLTQLPEKLAQMEALGLDQAVVIHFTREVAGKDAR
                     TFLTELMRSLPIVELWVGEGFALGHHRTGNIEVLEALGEELGFGLRVFPPVREGETVI
                     SSTAIREALAAGDVEQATDMLGRFYAVPGIVVPGARRGRELGFPTANTTPPANQAIPA
                     DGIYATMTRRPRTGERLPSITSIGVRPTFGPSARLIEVYILDFADDLYGEQLCIEFVD
                     YLRPQVAYTGPEPLIRQMHEDLARTREILAALPGA"
     gene            complement(3302..3835)
                     /locus_tag="VF276_13950"
     CDS             complement(3302..3835)
                     /locus_tag="VF276_13950"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX6608003.1"
                     /translation="MGNRERGGTVAAPQTVAGAPAIEHDYLQIGEVAELTGLTQRTLR
                     YYEELQLLDPPTRMAGGFRLYSPADVARVQHIVRLKQLLGFSLAEIKEIVTAEEAQAD
                     LQAGSDHAASARRLARLDHLIATTNSQLDMLARKIGQMEVLRGELAGRLRELESRRAA
                     LLIEHEGAQLHAASEYS"
     gene            complement(4148..5224)
                     /locus_tag="VF276_13955"
     CDS             complement(4148..5224)
                     /locus_tag="VF276_13955"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HEX6608004.1"
                     /translation="MARLDVWDSIGRMTGRGRAARSGGQNENGGTPPGTGPRKRWPRL
                     LALGLIVCVLLAGAANALRGAASSGSPVLPRSGTQPFVAPVPTLVPAADMQKASAEAG
                     MPAPASGGASATGSGQAAPGAQWDRMIIRNATLQLKVKDVAASMDAVRGVASQHNGYI
                     TQSESHQDGNYTVATITVQVPAKEFDAVMPELRRLGVKTLQENVTSSDVTDEYTDLQS
                     QLRNLQATEARMLALQQKAGTLEDVLAVDRELRQVQGQIEQIQGRSNYLSKRAEMSSI
                     TVTLSPDLVPADPAPQPATGWDPAAIAQHAWEASLDTLAGAGSVVISVVVFFWWAFPL
                     VFLLWLVLRTRRNADRSPSPGEIA"
     gene            5653..6375
                     /locus_tag="VF276_13960"
     CDS             5653..6375
                     /locus_tag="VF276_13960"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEX6608005.1"
                     /translation="MLDIQIAVSKINKYATRESGDTVEVVERPLGGFTLMLVDGQGSG
                     RASKLLSQLVASKVLALIKDGARDGVAARAAHDYLYLQRAGKVSATLALCTVDLSTRS
                     IVLTRNSHCPIAVYGPEGNGLRWLDAEAQPLGLYRNTRPVVAEIDLAPGTCIVTFSDG
                     ILEAGRRAGRDWDVRATLAGLLTPDAPPPAPQALADRILAGAMAADQDRPQDDMSVLA
                     LTIGEREDAPQIRTLHMSVPAD"
     gene            6387..7076
                     /locus_tag="VF276_13965"
     CDS             6387..7076
                     /locus_tag="VF276_13965"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEX6608006.1"
                     /translation="MDSAPSEAGFTADLRTAAEPIWRQQAAHPFLRGLADGTLPAAKF
                     LFYTRQDAVFLQELNRVFGYGAARADTGADQAHFARLLIETQQILDDLHGRYAAGQGV
                     DAATLAATRPAAVTYAYSRHLLSTAVADSYPALIAALLPSMWMYDELGHALAAQGAPP
                     ADHPYHDWLVVHAGPHLQTITTWMRSLLDERAPTLPAVERAHLQDVFLISSRYEILFW
                     EMAWREEEWPV"
     gene            7322..>7621
                     /locus_tag="VF276_13970"
     CDS             7322..>7621
                     /locus_tag="VF276_13970"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX6608007.1"
                     /translation="MSTNYGEAHNEWRNYRVAHGALIEDGQILLAGNRWYPDQPLVWS
                     LPGGRGEDGEPVIEAVVREFREETGLEVAAGPLIYVAEARSVVRRQIFLTCAFAVR"
BASE COUNT         1220 a         2640 c         2522 g         1239 t
ORIGIN      
        1 agcggcttcc tgcgccggga actggccgag cgcatcgatc tgcgctacgc gccggagatc
       61 agcttccgca acgatccctc ggtggccaat agtgatcgta tcgcgcgcct gctcacggag
      121 attgagcagg agaaagactc agcgaactga tgtccgccga cctgcccgct accgcggctg
      181 ccgcgcctaa aaccacaatc cgtaattccg ccgagcggcc gctgggagtc gaagactacc
      241 acgcggccgc cgcgtttatc cgccgttatc accgcatctg gatggtgctg catgtcagcc
      301 ccgacggcga ctcgctgggc agcaacatgg gcctggccca tatgctgcgc caggccggcc
      361 acgacgtgac cgtgctgtcc tccgacgcgc tgcccgagta tttcgcgccc ttcttgctcg
      421 acaaggcggc accccaggtg gtaatcggcg ccactgcgcc cggcccgccg cccgacgcct
      481 gggtcattct cgacaccagc gacccgatcc ggctcggcaa cgtctacaca gcgaaccaga
      541 agctgctggc cgagcggccc gtgctcaacc tggatcacca cccgaccaat ctcgaatacg
      601 gccagttgaa cctgatcgat tcgtccgcgg cggcggtcgg cgagcagatc ccccagttgc
      661 tgcgcgcgct cgacctgacc ccggacgcca ccgccgcgaa ctggctgctg ctgggcatcg
      721 tcaccgacac gctcggcttc cgcacatcga acacgacggc gcgcaccatg agcaccgccg
      781 cccagttgat ggagcgcggg gcgcagctct acgcgatcaa cgaggcgatc ttcaacacgc
      841 gcccgctctc ccaggtgctg ctctggagcc gcgcccttgc cacgatcaag accgacgggc
      901 gcacgctctg gaccacgatc agccgcgaga tgctgcgcga aacgggcgcg cgcgaggaag
      961 aaaccgaagg gctggcctcg ttcctgtcgg gcgtgcgctc gatgaaggtc ttcgccttgc
     1021 tcaaagagcg gggcgacggc acccgcgtca gcctgcgctc gaatccgggt accgacgtgt
     1081 cggccatcgc catgcgcttc gggggcggcg ggcacaagca ggccgcgggc tgcaccatcc
     1141 ccagcatcgg cgaggccgcc gagcaacaat tgctggccgc cgtggccgag gtgctgggcc
     1201 cgctggagag tgctgagtag agccgtcagc cgtcagccgt cagctaggtg cctgtggcat
     1261 cgctgcgcga tcagccgtca gccatcggct atcagttttt actgaccact gaccactgac
     1321 ggctgacggc tatgtacgat ccggcaatat gttatgatgg ggcattacgt tgcaagcctg
     1381 ggcgcaatcg accgttaccg acgtttacca caggctggac cattgaccgg agcacagggg
     1441 accccgcccg cgacgggcgt gctggtgatc gacaaaccgc tgggccgcac ctcgtttgat
     1501 gtggtggcgc gcgtgcgccg ggtctgcggc cagaagcgcg tggggcacgc cgggacgttg
     1561 gatccgctgg cgacgggcgt gctgcccatc tgcctgggca cggcgacccg gctggtggaa
     1621 tacctggccg atgcgggcaa ggagtaccgg gcgcgactcc acctgggcgc cgtcagcgcc
     1681 acatatgacg gcgaggggcc tgtcacgccg caagtgaccg acccggcctt gctgccggcg
     1741 acagcgacga tcctggccgc gctggccgcg tttcgcgggc cgcagcagca ggtgccgccc
     1801 atgcactccg ccgtgcagca gggcggcaag cggctctacg aactggcccg cagcggcgtc
     1861 gaggtcgaac gccggccccg cgccgtgacg atctaccggc tggaactggt ggcctatacg
     1921 ccgccggaac tggtgctgga tgtcgcgtgc agcaaaggca cctacatccg cagcctggcc
     1981 catgatctcg gggcagccct gggctgcggc gcctacctgg ccgggttggt gcgcacccgg
     2041 cacggcccgt tcgggctggc cgatgccgtc acgctggacc agttagagca cgccgtggcc
     2101 gcgggccgcc tgcacgaggt gctgcgcccg cccgatgtgc tggtggccga ttggccgcgc
     2161 gtcgcggtcg cggcggatgc cgcccgccgg ctggcgcaag gccaggcgct cgactacccg
     2221 ccgcccgccg gcaacgcgcc ccggcggtta cgcgtatatg atcccgacgg cgcgttcctg
     2281 gcgctggtgc tctgggaaga cgacaaggcc cgctggcacc ccgccaaggt cttcagcggc
     2341 gtttagaggc tttgagggta aggacgcgaa tgcaagtaat caccgatctc gaacacttcc
     2401 actccgcgcc ggcggtgctg accatcggca acttcgacgg cgtgcatcgc ggccaccagc
     2461 aactgctggg cctggtgcgc gaccgcgccc gcgacctggg cgtccgctcg acggtgctca
     2521 ccttcgaccc gcacacccgc gccgtggtgc ggcccgatca gccgttgcag ttgctgacgc
     2581 agttgccgga aaagctggcc cagatggaag cgctgggcct cgatcaggcg gtggtcatcc
     2641 acttcacccg cgaggtcgcg ggcaaggatg cacgcacgtt cctgaccgaa ctgatgcgtt
     2701 cgttgcccat cgtggaactc tgggtaggcg agggctttgc cctgggccac caccgcacgg
     2761 gcaacatcga agtgctggaa gcgctgggcg aggagttagg cttcggcctg cgggtcttcc
     2821 ccccggtgcg cgagggcgag acggtcatca gcagcaccgc gatccgcgag gcgctggcag
     2881 ctggggatgt ggagcaggcc accgacatgt tgggccgctt ctatgcggtg ccgggcatcg
     2941 tcgtgccggg ggcgcgacga gggcgggaac tgggctttcc gacggcaaac accacgccac
     3001 ccgccaatca ggccatcccg gcggacggca tctacgccac gatgacgcgc cgcccgcgca
     3061 ccggggagcg cctgcccagc atcaccagca tcggcgtgcg cccgaccttc ggccccagcg
     3121 cacggctgat cgaagtctat atcctcgatt ttgcggacga tctgtatggg gagcaactct
     3181 gcatcgagtt tgtcgactac ctgcggcccc aggtggccta cacggggccg gagccgctga
     3241 tcaggcaaat gcacgaggac ctggcgcgca cgcgcgagat cctggccgcc ctgcccggcg
     3301 cctagctgta ctcggacgcg gcgtgcaact gggcgccctc gtgctcgatg agcagcgcgg
     3361 cgcgccggct ttcgagttcc cgcaagcggc cggccagttc gccgcgtaac acttccatct
     3421 ggccgatctt gcgcgccagc atgtcgagtt gggagttcgt ggtggcgatc aggtggtcca
     3481 gccgggccag gcggcgcgcg ctggccgcgt ggtcgctgcc cgcttgcagg tcggcctgcg
     3541 cctcttccgc ggtgacgatt tccttgatct cggccagcga gaagcccagc agttgcttga
     3601 gccgcacgat atgttgcacc cgcgccacat cggcggggga atagaggcgg aagccgcccg
     3661 ccatacgcgt gggcggatcg agcagttgga gttcttcgta gtagcgcagc gtccgctggg
     3721 tcagcccggt tagctcggcg acctccccga tctgcaaata atcatgctcg atcgcgggcg
     3781 cccccgcgac ggtctgcggc gcggccaccg tgccgccgcg ctccctgttt cccataccca
     3841 ccttgccagt cccccgatcc taccctgtga taactgtgac catcaactct atcattaacg
     3901 taaacgttaa cgtcggcaca gcggatctgg tactccccta cccggtaggc cgccagcgct
     3961 tcaattcggg tttgtcactc ttcgctccgc tcagagtctc gtctcgtacg gaagaagacg
     4021 agatgcgtca ctgcgttcag catgacaatt cgggtttgaa gcacggcgcg cactgttcta
     4081 gcacagggac agatccaccg ggcatgaaaa cggctgccgg atgggcatcc ggcagccggg
     4141 gtgtgtgtca ggcgatctcg ccgggggacg gcgagcggtc ggcgttgcgg cgcgtgcgca
     4201 gcaccagcca gagcaggaag accagcggga aggcccacca gaagaagacc accacgctga
     4261 tgactacgct gccggcaccg gccagcgtgt ccagcgaggc ttcccaggcg tgctgcgcga
     4321 tggcggcggg atcccagccg gtcgcgggct gcggcgcagg gtcggccggc acgaggtccg
     4381 gggagagcgt gacggtgatg ctcgacatct cggcgcgctt gctcaggtag ttgctgcggc
     4441 cctggatctg ctcgatctgg ccctgcacct ggcgcaactc gcggtcgacc gccagcacgt
     4501 cttccagcgt accggctttc tgctggagcg ccagcatgcg cgcctcggtc gcctgcaagt
     4561 tgcgcagttg cgactgcagg tctgtgtatt cgtcagtcac gtcgctgctg gtgacgttct
     4621 cctgcagcgt cttcacgccc agccggcgca gttcgggcat cacggcgtcg aactccttgg
     4681 cgggcacctg cacggtgatc gtcgccacgg tgtaattgcc gtcctggtgg ctctccgact
     4741 gcgtgatata gccgttgtgc tggctcgcca cgccgcggac ggcgtccatg ctcgccgcca
     4801 catccttgac cttgagttgc agcgtggcat tgcggataat catgcggtcc cactgcgcgc
     4861 cgggtgcggc ttgcccgctg cccgtggcgc tcgcaccgcc actggcgggg gccggcatgc
     4921 ccgcctcggc agacgccttt tgcatatccg ccgccggcac caatgtgggt acgggtgcga
     4981 caaacggttg ggtaccgcta cgcggcagca caggggaacc cgacgatgcc gcgccccgca
     5041 gcgcgttcgc ggccccggcc aggagcacgc acacaatcaa gccaagcgcc agcagccggg
     5101 gccaccgctt gcgcggcccg gtgcccggcg gggtcccgcc gttctcgttc tgtcctcccg
     5161 accgcgcggc gcgcccccgc ccggtcatgc gcccgataga gtcccaaaca tccagacgtg
     5221 ccattggtcg cctcctatgg gtacgattgc tcaaatatgg gtgcgcgcgc tgcttacacc
     5281 tacaagacgc cggagggccg tgaaatagtt cccgacttaa cgatccgatc tgccctgcgc
     5341 ggtctgcaaa gcattcatat tcaccgcgga gacgcagaga acgcagagat cattaacgga
     5401 agttctgttt aggagatttc tcgggggcgg cgcccagccg gggccgcgtt tggctggaac
     5461 ccaggcgtct ttgtgctgca ttccccgccg gatctccttc aaatctctgc gttctctgcg
     5521 tctctgcggt gcgcatcctg attttgcgcg gatgtgcggc ccggcgcatt gcccggcccg
     5581 gcggcatgtg gtatgatccc ggcataccgc agccgatagg ctggcgccgg cagccccggc
     5641 acagggagcg caatgctcga tatccagatc gccgtcagca agattaacaa atacgccacg
     5701 cgggagagcg gcgacaccgt cgaggttgtc gagcgcccgc tgggcggctt cacgctgatg
     5761 ctggtcgatg gtcagggtag cgggcgcgcc tccaagctgc tcagccagct ggtcgccagc
     5821 aaggtgctgg cgctgatcaa ggatggcgcc cgcgacggcg tggccgcccg cgccgcgcac
     5881 gattacctct acctccagcg cgcgggcaaa gtctcggcca ccctcgcgct ctgcacggtg
     5941 gacctcagca cgcgcagcat cgttctgacc cgcaacagcc attgccccat cgccgtctat
     6001 ggtccggagg gtaacgggct gcgctggctg gacgcggagg cccagccgct cggcctctac
     6061 cgcaacacgc ggcctgttgt cgcggagatc gacctggcgc ccggcacctg catcgtcacg
     6121 ttcagcgacg gcatcctgga agcaggccgg cgcgccgggc gcgactggga cgtgcgcgcg
     6181 accctggccg ggttgctcac accggacgcc ccgccgcccg ccccgcaggc gctggccgat
     6241 cgcatcctgg ccggcgccat ggccgcggac caggaccgcc cgcaggacga tatgagtgtg
     6301 ctggcgctga ccatcggcga gcgcgaggac gcgccgcaga tccgcacgct gcacatgagc
     6361 gtgcccgccg attgatggag aatcccgtgg actctgcccc ttccgaagcc ggattcaccg
     6421 ccgacctgcg tactgccgcc gagccgatct ggcggcaaca agccgcgcat cccttcctgc
     6481 gcggcctggc cgacggcacg ctgcccgccg ccaagttcct gttctacaca cggcaggacg
     6541 cggttttctt acaggaactg aaccgcgtct tcggctacgg cgccgcgcgc gccgacaccg
     6601 gcgccgacca ggcgcacttc gcccgcctgc tgattgagac ccagcagatc ctggacgatc
     6661 tgcatggccg ctacgccgcg ggccagggcg tggatgccgc gaccctggct gccacccgcc
     6721 ccgccgccgt tacctacgcc tactcgcgcc acctgctcag caccgccgtg gcggacagct
     6781 atccggccct gatcgccgcc ctgctgccga gcatgtggat gtacgacgaa ctgggccacg
     6841 cgctcgccgc gcagggcgcc ccacccgccg atcatcccta ccacgactgg ctggtggtcc
     6901 atgccgggcc gcatctgcaa accatcacga cgtggatgcg cagcctgctc gacgagcgcg
     6961 cgcccaccct ccccgccgtc gagcgtgccc acctgcagga tgtcttcctg atcagcagcc
     7021 gctacgagat cctgttctgg gagatggcct ggcgcgagga ggagtggccc gtatagccat
     7081 cagctatcag ccgtcagccg tcaggaaacg acggtagaca ggctgtcact ctgagcgcag
     7141 cgaagagtct cgttgcgccc tggctgggcg ggacagcggc gggcgcagga tggggtgtca
     7201 ctctgagcgc agcgaagagt ctcgtcgcgc ctcaccagga cggagactct tcgctgcgct
     7261 cagagtgaca agggaagcgg aattaaaaac ctgacggctg atagctgaaa gctgacgcat
     7321 tatgtcaacc aactacggcg aagcgcataa cgagtggcgc aactatcgcg tggcgcatgg
     7381 ggcgctgatc gaggatggac agattctgct ggcgggcaac cgctggtatc ccgaccagcc
     7441 gctggtctgg agtctgcccg gcgggcgcgg cgaggacggc gagccggtga tcgaggccgt
     7501 ggtgcgcgag ttccgcgagg agaccgggct ggaggtcgcg gcgggaccgc tgatctacgt
     7561 ggccgaggcg cgctctgttg tgcgccgcca gatcttcctc acctgcgcct tcgccgtgcg
     7621 c
//
LOCUS       DASUYC010000849         3133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_829320,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000849 DASUYC010000000
VERSION     DASUYC010000849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829320"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(305..1117)
                     /gene="trpA"
                     /locus_tag="VF276_13975"
     CDS             complement(305..1117)
                     /gene="trpA"
                     /locus_tag="VF276_13975"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX6608008.1"
                     /translation="MGTERIAAAFAAARARGEVALIPYVSAGYPTLDKSAELIRVLAD
                     NGADLIEIGIPFSDPLADGATIQRASQVALQGGTNLDRCLDMVRALRPQVAAPLIFMG
                     YYNPLHHRGVARAVADCAAVGVDGLIVPDLPPEEAGDLLAACAEHNIALIFLVSPVSS
                     EDRLAEVAARASGFIYCVSLTGVTGARAELSHTLPEFIARVRRHTDLPLAVGFGLSTA
                     GHVAAVGRLADGAVVASALIDRITASPGAEAEAAAAFLRGLRGQEAPAPAAV"
     gene            complement(1117..2364)
                     /gene="trpB"
                     /locus_tag="VF276_13980"
     CDS             complement(1117..2364)
                     /gene="trpB"
                     /locus_tag="VF276_13980"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX6608009.1"
                     /translation="MTTLANTPTGAVLPDAGGHYGAFGGKYVPETLMPALHELETAYA
                     AIQADAGFAAELAALQRDYVGRPTPLYHARRLSAEAGGAQIYLKREDLAHTGAHKINN
                     ALGQALLAKRMGKQRIIAETGAGQHGVATATACALLGLDCIVYMGEVDIARQAPNVFR
                     MKLLGAEVRPVTSGSRTLKDAINEAIRDWVTNVENTFYIIGSVVGPHPYPRMVRDFQA
                     VIGQEARAQSLAQIGRLPDAVVACVGGGSNSMGIFYPFVNDPTVRLIGVEAGGQGLAS
                     GKHAAPLSAGTPGVLHGARSYLMQDADGQVLETHSISAGLDYPGVGPEHSYLKDSGRA
                     EYVAATDDEAMQGFQALCRLEGIIPALESSHAVYYAMHLAPQLGPDAAIVVNLSGRGD
                     KDIYTVAETLGVRLSGAAGGETK"
     gene            complement(2484..>3133)
                     /locus_tag="VF276_13985"
     CDS             complement(2484..>3133)
                     /locus_tag="VF276_13985"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEX6608010.1"
                     /translation="KRRVTPAEAAALIAGLHAQPGAAAPLAVGLFVNTPPEEIAATVA
                     ATGVDLVQLCGDEPPDAQALVTIGCPVIRIVRATPATFAEAEAAIAAWQTAAAVADGL
                     AGPVRGPWGSRLLIGLDAPHAGAYGGTGVAADWTLAATLAARHPIMLAGGLTPENVAA
                     AVAQVRPWAVDVSSGVETAGVKDPARIAAFAERAVSGQPSAISGQPSAISGQVTA"
BASE COUNT          447 a         1031 c         1178 g          477 t
ORIGIN      
        1 tttcaaaccg gcctgccgcg gtcgatacgc gacgcgctta cgactgcttc ccgtctactt
       61 tgtcattctg agcggagcga agaatctcgt ctcgttgggg gccaagacga gattcttcgc
      121 tccgctcaga atgacaaagt agacgggaag cagtcgtaag cgcgtcgcgt atcgaccgcg
      181 gcaggccggt ttgaaaccgg cccctacaga ggaatattgc acgcaaaggg cggtcggtat
      241 caaccgccct cgcgtattca tgatgatcgc gatcacacgc cgctacccgg cggttcgcgc
      301 cccgctagac ggcggcgggg gcgggcgcct cctggccgcg caggccgcgc aggaaagcgg
      361 cggcggcttc ggcctcggcg ccgggcgacg cggtgatgcg gtcgatcagc gcggaggcga
      421 ccacggcgcc gtcggccagg cggcccaccg cggcgacgtg gccggcggtg ctcaggccga
      481 agccgacggc gagcgggagg tcggtgtggc ggcgtacgcg ggcgataaac tcgggcaggg
      541 tgtgcgacag ctcggcgcgc gccccggtca cgccggtgag ggagacgcag tagatgaagc
      601 cgctggctcg cgcggccacc tcggccagcc ggtcctcgct gctgaccggc gagaccagga
      661 agatcagggc gatgttgtgc tcggcgcagg cggccagcag gtcgcccgct tcttcgggcg
      721 gcaggtcggg cacgatcagg ccgtccaccc cgacggcggc gcaatcagcc acggcgcggg
      781 ccacgccccg gtggtgcagc gggttatagt agcccatgaa gatgagcggg gcggcgacct
      841 gcgggcgcaa cgcgcgcacc atgtcgaggc agcggtcgag gttggtgccg ccttgcaggg
      901 ccacctggct cgcgcgctgg atcgtcgcgc cgtcggccag cgggtcgctg aaggggatgc
      961 cgatctcgat caggtcggcg ccgttgtcgg ccaggacccg gatcagctcg gcgcttttgt
     1021 ccagggtggg atagcccgcc gagacatagg gaatcagcgc gacttcgcca cgcgcgcggg
     1081 cggcggcaaa ggccgccgcg atacgttcgg tgcccattac tttgtctccc ctccagccgc
     1141 cccgctcagg cgcacgccca gcgtttcggc cacggtatag atgtccttat cgccccggcc
     1201 cgacagattg accacgatgg cggcatcagg gccgagttgc ggcgccaggt gcatggcgta
     1261 gtagaccgcg tgcgacgact ccagggccgg gataatgccc tccaggcggc agagggcctg
     1321 gaagccctgc atcgcctcgt cgtcggtggc cgcgacgtac tcggctcgcc cgctgtcctt
     1381 gaggtaggag tgttccggcc cgacgccggg gtagtccagc cccgccgaga tggagtgggt
     1441 ttcgagcacc tggccgtcgg catcctgcat gaggtacgag cgcgccccgt ggagcacgcc
     1501 gggcgtgccc gcggacagcg gcgcggcgtg cttgccgctc gccagtccct gcccgcccgc
     1561 ctccacaccg atcaggcgca cggtggggtc gttgacgaag gggtagaaga tgcccatgga
     1621 gttgctgccg ccgcccacgc aggcgaccac ggcgtcgggc aggcggccaa tctgggccag
     1681 ggactgggcg cgggcctcct ggccgatcac cgcctggaag tcgcgcacca tgcgcgggta
     1741 ggggtgcggg ccgacgaccg agccgatgat atagaacgtg ttctcgacgt tcgtcaccca
     1801 gtcccgaatc gcctcgttga tggcgtcctt cagcgtgcgg ctgccgctgg tgacggggcg
     1861 cacctcggcg ccgagcagct tcatgcggaa gacgttgggc gcctggcggg cgatgtcgac
     1921 ttcgcccatg tagacgatgc aatcgaggcc gagcagggcg cacgcggtgg ccgtggcaac
     1981 gccgtgctgg cccgcgccgg tctcggcgat gatgcgctgc ttgcccatgc gcttggcgag
     2041 cagggcctgg cccagcgcgt tgttgatctt gtgcgcgccc gtatgagcga gatcctcgcg
     2101 cttgagatag atctgcgcgc ccccggcctc ggcgctcagg cggcgggcgt ggtagagcgg
     2161 cgtgggccgg cccacgtagt cgcgctgcag cgcggccagt tcggccgcga acccggcgtc
     2221 ggcctggatg gcggcatagg cggtttcgag ttcgtgcagc gcgggcataa gcgtttcggg
     2281 cacatacttg ccgccgaacg cgccgtaatg gccgccggca tcgggcagca cggccccggt
     2341 gggggtgttg gctagggtgg tcatgggtgc ctccatatag ctgtcagcca tcagccgtca
     2401 gcgttcaggt gtttgtgatt gggcataaag tcgataagcc gagtgcatca ctcgtcagcc
     2461 ggttatcgat tcctggctat cagctatgcc gtgacctgac cgctgatagc tgacggctga
     2521 ccgctgatag ctgacggctg accgctgacg gctcgttcgg cgaaagccgc gatgcgggcg
     2581 ggatctttga cgcccgccgt ttcgacgccg ctgctgacat cgacggccca gggccgcacc
     2641 tgggcgacgg cggcggcgac attctccggt gtgaggcccc ccgcgagcat gatcgggtgg
     2701 cgggcggcca gggtggcggc cagggtccag tcggcggcga cgccggtgcc gccgtaggcg
     2761 cccgcgtggg gggcgtcgag gccgatcagc aggcggctgc cccacggccc gcgcaccggg
     2821 cccgccagtc cgtcggcgac ggcggcggcc gtctgccagg cggcgatggc ggcctcggcc
     2881 tcggcgaagg tggcgggcgt ggcgcgcacg atgcggatga ccgggcagcc gatggtgacg
     2941 agggcctggg catcgggcgg ctcgtcgccg caaagctgca ccagatcgac gccggtggcg
     3001 gcgaccgtgg cggcgatctc ctccggcggc gtgttgacga agaggcccac ggccagcggc
     3061 gcggcggcgc ccggttgcgc gtgcagcccg gctatcagcg cggcggcctc ggcgggcgtg
     3121 acccggcgct tgc
//
LOCUS       DASUYC010000850         2416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_829344,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000850 DASUYC010000000
VERSION     DASUYC010000850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829344"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..1209)
                     /locus_tag="VF276_13990"
     CDS             complement(190..1209)
                     /locus_tag="VF276_13990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX6608011.1"
                     /translation="MYFDAVIQPPELRDAGPTAAAAERLGLQALWTTEIQHDPFLPLG
                     LAAVATERVHLGTAIALAFPRSPTAMAYTAWDLARASQGRFILGLGTQVKAHIERRFA
                     APWDAPVARLRDYIAAMRAVWASWQTGERLRHEGPYYTLKLMTPFFNPGPLPYGEPPV
                     FIAGVNHGLCRLAGETCQGFHVHPFHTARYLRENVLPWIGEGLAAAGRERGAIQVSAS
                     VFVITGQGAARAAMREAMRRQVAFYASTPSYATLLDLHGWADRGAALAQLAARGRWDE
                     MGAVIDDAMLAEFAVEGDTLADAAAAILARYAGLLDRAAFYLPFAPGERDAEWAAAAD
                     VFARG"
     gene            1775..2239
                     /locus_tag="VF276_13995"
     CDS             1775..2239
                     /locus_tag="VF276_13995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608012.1"
                     /translation="MNCPACGGSLPLPAAGAQYIRCSYCRSSLRLRRDGDTLRLELAY
                     RPGPPVGRDGLLPDGPLGLQPAAQLQQLLLELPALRERVATLQRGGGGLELVEAKRQL
                     AAVEDQIWALQLDLYPEGARQAGPARVTLMPRLLGVAAVAALVVGMALIFLH"
BASE COUNT          373 a          812 c          866 g          365 t
ORIGIN      
        1 gacgcgccag acactccata agctggatca gcagggcctg atgcgcagga gtcagccgct
       61 cccacaaagc ttgtacgcgc ggtgacatct ggaccctcct ttcctttggt cgaatatgcc
      121 ggtgcaacag cacagtatag gagacaagct ctgccgccat caagaacgtg accgcaggtc
      181 gggcctccac tagccccgcg cgaacacgtc tgccgccgcg gcccactcgg cgtcacgctc
      241 gccgggggcg aacggcagat agaaggcggc ccggtcgagc aacccggcgt agcgggccag
      301 gatcgcggct gcggcgtcgg cgagcgtgtc gccctccacc gcgaattcgg ccagcatggc
      361 gtcgtcgata acggcgccca tctcgtccca gcgcccgcgg gccgccagtt gggccagcgc
      421 ggcgccccga tccgcccagc cgtgcaggtc gagcagagtc gcgtagctcg gcgtcgaggc
      481 gtagaaggcg acctggcggc gcatcgcctc gcgcatggcg gcccgcgcgg ccccctggcc
      541 agtgatcacg aagacgctgg cgctgacctg gatcgcgccg cgctcgcgcc cggcagcggc
      601 cagcccttcg ccgatccacg gcagcacgtt ctcgcgcagg tagcgggcgg tgtggaaggg
      661 atgcacatgg aagccctggc aggtttcgcc ggcgaggcgg cacagcccat ggttcacccc
      721 ggcgataaag acgggcggct cgccgtaggg cagcggcccc ggattgaaga agggcgtcat
      781 caacttgagc gtgtaatacg gcccttcgtg gcgcaggcgc tctcccgtct gccaggaggc
      841 ccagacggcg cgcatggcgg cgatgtagtc gcgcaggcgg gcgacgggcg catcccaggg
      901 cgcggcgaag cggcgctcga tatgggcttt gacctgggtg cccaggccga ggatgaagcg
      961 gccctgggat gcgcgcgcca ggtcccaggc ggtataggcc atggcggtgg ggctgcgcgg
     1021 gaaggccagc gcgatggcgg tgcccaggtg cacgcgctcg gtggcgacgg cggccagacc
     1081 cagcggcaag aacgggtcgt gctgaatctc ggtggtccag agcgcctgta gccccaggcg
     1141 ttcggcggcg gcggcggtcg gcccggcatc gcgcagttca ggcggctgga taacagcatc
     1201 aaagtacatg agcggctccc ttcccggcgc ggtgcggccc ggaaggaaag ccgccgggcg
     1261 ctgccgggtg tatatgcttg agtccgcgcc attataacac agccgggcgg cgcggcccga
     1321 gcccaggccc cgccgggcgg ccccatgtca caccggcgaa acccgtggct ggggcacgaa
     1381 ggtctactgc cggccaacct cgctgcgggc gcccgtcgcg ccggcaggca tactggctga
     1441 gaagcgaatt gcccgatgaa agggatagcg cgatttggta aagtttggta aactgagatc
     1501 aacacccttc gcacgtgccg ccgcgacgga gggcgctgac ggttagtagc atgtagaacg
     1561 gtgcctggtc ccctgtcccg tgcgggcgcc ggccaatcca atggcattct ccacccctac
     1621 ctagtgattg gatgtgccca tggctacttt tacgcagtgc ccggcttgtg gtgagccggt
     1681 tcaaccgttc tggcgcgatt gcccgcaatg tggggtggcc ttcccccagg gggcgacaag
     1741 cggcgcgccc ctccgggcac cggcgatcgg cctggtgaac tgtcccgcct gcggggggtc
     1801 gctgccgttg cccgcggcgg gtgcccagta catacgctgc tcctactgtc gctccagcct
     1861 gcgcctgcgg cgggacggcg acacgctgcg cctcgaactg gcctaccggc cggggccgcc
     1921 cgtggggcgc gacggcctct tacccgatgg gccgttaggc ttgcaacccg cggcgcaact
     1981 gcagcagttg ctgctggaac tgcccgcgct gcgcgagcgg gtcgcgacgc tgcagcgggg
     2041 gggcggaggc ctcgaactgg tcgaagccaa acgccaactc gcagccgtcg aggaccagat
     2101 ctgggctttg caactcgacc tgtaccccga gggagcgcgg caagcggggc cggcccgcgt
     2161 gacattgatg ccccggctgc tgggcgtggc ggcggtggcg gcgctggtgg tgggcatggc
     2221 gctgatcttc ttgcactagc cggcatctcc ctgtgctctg cgggccggtt tgaaaccggt
     2281 ccctacacct gcatcggcgc tgggcgccgg gccggtttca aaccggtccc tacacctgca
     2341 tcggcgctgg atggcgggcc gattccaaat tggtccctac acccgccctg gcgctgggcg
     2401 acgggccggt ttgaaa
//
LOCUS       DASUYC010000851         2877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1288716, whole genome shotgun sequence.
ACCESSION   DASUYC010000851 DASUYC010000000
VERSION     DASUYC010000851.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1288716"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VF276_14000"
     CDS             complement(<1..390)
                     /locus_tag="VF276_14000"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEX6608013.1"
                     /translation="MSEQVLPAAPLRLAEAYALGFLRQGRAGWDEPHTRAVVHYAGAL
                     ARAAGVDLLVAVTVGWLHDIGYAGLFAAADSSRHAVVRSRKALHMERGAALARAFVDR
                     PDVAPFYTPAQRDRIVHLVSVHDKVELL"
     gene            complement(564..>2877)
                     /locus_tag="VF276_14005"
     CDS             complement(564..>2877)
                     /locus_tag="VF276_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608014.1"
                     /translation="DTDGKMYRVQYFERAIFEYHPENATPYDVLLTLLGVFRYRQQYG
                     PAGGPDQHANPANSRRFPETGYHMGGDFRTYWEQHGGLAQQGYPISEEFTEVSPLDGK
                     PYTVQYFQRAVFERHPEHAGTPYEVLLSQLGTFRYQARTTGIAIPAPGPGRVQYALQG
                     AGPYLVWKESRGKFGDQVDLRALDTRTNRLIFVADVKGEGSDPAASGSLVVWQQVAAC
                     APDCGTNDILGKDLATGASYTIATGLADRTAPVVSGRHVAWQTTSNTELWLKDIDTGA
                     LIRVAVLPPYGGTYLTYPVLTERYLAWILWEPRTEGEQHYMVQVYDLATQTTRTLADR
                     TIRSASQNLHLAVSGERLVWSDPAAHLADLTTGAVTDLPVEYAADITIQGDTVLWSAK
                     GAPNATDLDIWGMRLLDSRPLLLASAPGNQQRPAVAGDWLTWQTQGGPQDGRLGAARL
                     ADAFLRPDAPPLTPPVTAGLITTTVRRAAWGAGHYLFWADSSYPYTRLYGYDLNTGRQ
                     WLLTEHLSTRYSVASDGQIVAWVEGEAGNSVASYNLNTGTASTIIPLGEPDLLWVEGI
                     AVDSGTLYYTANTRTGSGLFARPVTGGPPAQLHNGRVLPPVAAGGNLLWAEEEGYGGK
                     QVHYTSTLHLRTPAYPAADILLPAGQQGYSGYAVSSNFVAWTDATPDGTLHLYSLAGA
                     ASRQIATGAGTPRVRGNQVAWSETPGYGRFGGNVWTYDIATGATRLIAGGQDPQAEVV
                     ALLDGPAVAYTVGQGSLLDLRLYAAPLPAP"
BASE COUNT          402 a          963 c         1045 g          467 t
ORIGIN      
        1 caacagttcg actttatcgt gcacactgac cagatggacg atgcggtcgc gctgggcggg
       61 cgtatagaag ggcgccacat cgggccggtc gacgaacgcc cgcgccaggg cggcgccgcg
      121 ctccatgtgt agcgccttgc ggctcctgac gaccgcgtgg cggctggagt ccgccgccgc
      181 gaacagcccg gcatagccga tgtcgtgcag ccagcccacc gtcaccgcca ccagcagatc
      241 gacgcccgcg gcgcgcgcca gcgccccggc ataatgcacg accgcgcggg tgtgcggctc
      301 gtcccagccg gcccgtccct ggcgcaggaa gcccagcgca taggcttccg ccagccgcaa
      361 gggcgcggcg ggcaggactt gttcgctcat gtacgaatta tactaccctc tccaatttcg
      421 tgaagcatcc gtactacgtc gcgccggggg atcgggttgc gccggggtgc caccttgcac
      481 agcgtgggcc tgtcacggac cggcagcctg atcttcgtga tcaggctgcc gggctgcttc
      541 acgcgcgtgc ccgttcctga cgattacggc gcgggtaggg gcgccgcgta gaggcgcagg
      601 tcgagtagcg acccctggcc cacggtgtag gccaccgccg gaccgtcgag cagggcgacc
      661 acctccgcct gcgggtcctg gcccccggcg atcaggcggg tcgcgccggt agcgatgtcg
      721 taggtccaga cgttgccgcc gaagcgccca tagccggggg tctcgctcca ggctacctga
      781 ttgccgcgga cgcgcggcgt gcccgcgccg gtcgcgatct ggcgggaggc ggcgccggcg
      841 agcgaataga ggtgcagggt gccgtccggc gtggcatccg tccaggcgac gaagttggac
      901 gagacggcat agccgctgta gccctgttgc ccggcgggca gcaggatgtc tgccgccggg
      961 taggcgggcg tgcgcaggtg cagcgtggag gtatagtgca cctgcttgcc gccgtagccc
     1021 tcttcttccg cccagagcag gttgccgccc gccgccactg gcggcagcac ccggccgttg
     1081 tgcagttgtg cgggcgggcc cccggtcacg gggcgggcga acaggcccga tccggtgcga
     1141 gtgtttgccg tgtagtacag agtgccgcta tccaccgcga tgccttcgac ccagagcagg
     1201 tccggttcgc ccagcgggat gatcgtactc gcggttccgg tgttcaggtt gtagctcgcc
     1261 acgctgttgc ccgcctcgcc ctccacccag gcgacgatct gcccgtcgct tgccacgctg
     1321 tagcgggtgc tcaggtgctc ggtcagcagc cactggcggc ccgtattcag gtcgtagccg
     1381 tacaggcggg tatacgggta gctgctgtcg gcccagaaca gatagtgccc cgcgccccag
     1441 gccgcccggc gcaccgttgt ggtgatcagg ccggcggtga ccggcggggt gagcggcggc
     1501 gcgtcggggc gaaggaaggc atcggccagc cgggcggcgc ccagccgccc atcttgcggg
     1561 ccgccttgcg tctgccaggt cagccaatcg ccggccacgg cgggccgctg ctggttgccg
     1621 ggcgccgatg ccagcaggag gggcctgctg tccagcaacc gcatgcccca gatgtccagg
     1681 tcggtggcgt tgggtgcgcc cttggcgctc cagagcaccg tgtcgccctg gatggtgatg
     1741 tccgccgcgt actcgacggg cagatcggtc acggcgccgg tggtcaggtc ggccaggtgc
     1801 gccgccgggt cactccagac cagccgctct ccgctgacgg ccagatggag gttctgcgag
     1861 gcgctacgga tcgtgcggtc ggccagagtg cgcgtggtct gcgtcgccag gtcatagacc
     1921 tggaccatgt aatgctgctc accctcggtg cgcggctccc agagtatcca ggccagatag
     1981 cgctcggtca gcaccgggta ggtcaggtag gtgccgccgt agggaggcag gacggcgacg
     2041 cggatgagcg cgccggtgtc gatgtccttc agccagagtt cggtgttgct cgtggtctgc
     2101 caggcgacgt gccgcccgct gaccaccggc gcggtgcggt cggccagccc ggtggcgatg
     2161 gtgtagctcg cgccggtcgc caggtccttg cccaggatat cgttcgtgcc gcagtccggc
     2221 gcacaggcgg cgacctgctg ccagacgacc agcgatccgg aggcggcggg gtcgctaccc
     2281 tcgcccttca cgtcggccac gaagatgagg cggttagtgc gcgtgtcgag ggcgcgcagg
     2341 tccacctggt cgccgaactt gccgcggctc tccttccaga cgaggtacgg tcccgcgccc
     2401 tgcagcgcat actgcacgcg gccgggaccg ggcgcgggga tggcgatccc ggtcgtgcgc
     2461 gcctggtacc gaaaagtacc gagctggctc agcagcactt cgtagggcgt gccggcgtgt
     2521 tcgggatggc gctcaaagac agcgcgctgg aagtattgca ccgtgtaggg cttgccgtcg
     2581 agcgggctga cctcggtgaa ctcctccgaa atggggtagc cctgctgggc cagcccgccg
     2641 tgctgctccc agtaggtgcg aaaatcgccg cccatgtggt agccggtttc ggggaaccgg
     2701 cggctattgg cggggttcgc gtgctgatcc ggcccacccg ccgggccgta ctgctgccgg
     2761 tagcggaaca cgcccagcaa cgtgagcagc acgtcgtagg gcgtggcgtt ctccgggtgg
     2821 tactcgaaga tggcccgctc gaaatactgg acgcggtaca tcttgccgtc ggtgtct
//
LOCUS       DASUYC010000852         4489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1983258, whole genome shotgun sequence.
ACCESSION   DASUYC010000852 DASUYC010000000
VERSION     DASUYC010000852.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1983258"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..637
                     /locus_tag="VF276_14010"
     CDS             218..637
                     /locus_tag="VF276_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608015.1"
                     /translation="MNYTSKLAPRPIPALFTIVLSLAILLGMPSQASAAATYRADDYY
                     LLVKSCTGTSCLRDAAWGPAEAQLRGLFKQQGWGKDPAIASHNPGSLTSWSSWATEHV
                     YYIDGSTAREIRAQLMSTYADLRTAGAIHVLRVEVAR"
     gene            complement(996..3617)
                     /gene="clpB"
                     /locus_tag="VF276_14015"
     CDS             complement(996..3617)
                     /gene="clpB"
                     /locus_tag="VF276_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEX6608016.1"
                     /translation="MNLQKYTEKAQEAVLAAQSLATEHNNTQIEPEHLLLALLEQDGG
                     VVPAILQKLNVPIAPLIQQTQSEIDRLARLSGGPAEPRVSARLNAVANKAETEAKNIK
                     DDFVSTEHLLLALIDEGGRAPAAQLLKAAGVTRDRVLQVLSQIRGNQRVTSQNPEATY
                     AALEKYGRDLTAQAEKGKLDPVIGRDEEIRRVIQVLSRRTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRGDVPEGLKNKKLIALDLGAMVAGAKFRGEFEERLKAVLKEISESEGEII
                     VFIDELHTVVGAGAAEGAMDASNMLKPMLARGELHMVGATTLDEYRKHIEKDAALERR
                     FQPVFVGEPTVEDTISILRGLRERYEVHHGVRITDSALVSAAVLSNRYITDRFLPDKA
                     IDLVDEAASRLRMENESMPVELDQVKRRVMQLEIERQALSKEKDEASKDRLQKIESTL
                     ANLKEEERRLTAQLQSERQGQQAVRGLKEQIEQANAELEAAQRNYDLNKAAEIQYGRL
                     PELQRQLEAAQKVESGPRKGGTLLREEVTEDDIAAVVSKWTGIPVTRMLEGELAKLLR
                     MEERLHLRVVGQDEAITAVANAVRRARAGLQDPNRPLGSFLFLGPTGVGKTELARALA
                     EFLFDDENAIVRIDMSEYTEKFAVSRLMGAPPGYVGYEEGGQLTEAVRRRPYSVVLFD
                     EIEKAHPEVFNVFLQILDDGRLTDSQGRVVDFKNTVIIMTSNVGSQWISELGMANADA
                     IRERVQEALRQQFRPEFLNRIDETVIFHQLTRDQLTRIVDIQLERVRRRLAERKITLD
                     LTEKAKEFLGNEGFDPVYGARPLKRTIQRRILDPLSLELLESHFRDGDTILADVQDGR
                     IVFSKAATPAEAVPATA"
     gene            3954..>4489
                     /locus_tag="VF276_14020"
     CDS             3954..>4489
                     /locus_tag="VF276_14020"
                     /inference="COORDINATES: protein motif:HMM:NF024572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6608017.1"
                     /translation="MDDTEDKAITTGNEDAVAEPAPGEDAEPVEEEVDLATIQDDPEA
                     LQRLGESYLEADRNAEAIAVYTRLIELKPADPEPLLTRLWAHMNAGDTEATRADAALL
                     RERFPDDAEMLANLGDVNLLLDDREQALYDLDRAATLAGDNNELLYRVARSFAMAEEQ
                     EPALALYDRLVEWKPREV"
BASE COUNT          720 a         1486 c         1420 g          863 t
ORIGIN      
        1 gctcacgtcc aagaaacgac atcctccggt ttctcgtccg cttcttcctc cgcttattct
       61 accacttccc ccttctgctg tcatcagtga cctgttttaa tgaggatgtt tcttgtcgcc
      121 agatcacacc gctgcaccgc ccccaaccag tagctgcgat gatgcggcga tgaggggttt
      181 ttcgcagata gcaggccaaa tcgcaaggag gcataagatg aattacacat ccaagttagc
      241 gccccgcccc attcccgcgc tgttcactat cgtgctctcg ctcgcgatcc ttctagggat
      301 gccgagccag gcaagcgcgg cggcgaccta tcgggccgac gactactatc ttctagtcaa
      361 gtcctgcacg gggacgagct gcctgagaga tgccgcgtgg ggcccggccg aggcgcaact
      421 gcgggggctg ttcaagcaac agggctgggg caaagatccg gccattgcat cacacaatcc
      481 tggcagtctg accagttgga gcagctgggc caccgaacat gtctactaca tcgacgggag
      541 caccgcccgg gagatcaggg cgcaactgat gagcacctac gccgatctgc gaaccgccgg
      601 cgccattcat gttctacgcg ttgaggttgc ccgctgaccc gttcggaggg ggcgagaata
      661 cgggacgtag acgatagcgt gcccaaccgt gcagcgggcc ccgcccgtgc ggttgggcac
      721 gcggagcgta cggtggagcg ggcggaggtg cgcgtgcacc attgccgagg cacgttgagg
      781 aggcgacact gcagcatatc cggacggcgg gggggatgaa tgtggtgcgg ctgatcgcct
      841 ggtggaaaga cagaccacga gcagcaaccc gccggttccc atttgcagca ctggcgcccg
      901 cgtagctgca attcgccagc aacatcaata ttagcggcaa agcaaagagc cgcacgagta
      961 ctcgtgcggc tctgcgattc gcgctgttat tgcagctagg cggtcgccgg gaccgcttcg
     1021 gcgggcgtgg ctgccttgct gaagacgatc cgcccgtcct gcacatcggc caggatggtg
     1081 tcgccgtcgc ggaagtggct ttccagcaac tccagcgaga gcgggtccag gatgcggcgc
     1141 tggatcgtcc gcttcagcgg gcgggcgccg tacaccgggt cgaagccctc gttgccgagg
     1201 aactccttgg ccttctcggt caggtcgagc gtgatcttgc gctcggccag ccgccgccgc
     1261 acgcgctcca actggatatc gacgatgcgc gtcaactggt cgcgggtcaa ctggtggaag
     1321 atgaccgtct cgtcgatgcg gttcaagaac tcgggccgga actgctggcg cagagcttcc
     1381 tgcacccgct cgcggatcgc atcggcgttc gccatgccca attcgctgat ccactggctg
     1441 cccacgttgc tggtcatgat gatgaccgtg ttcttgaagt cgaccacgcg gccctggctg
     1501 tcggtcagcc gcccatcgtc caggatctgc aagaagacgt tgaacacctc cggatgcgcc
     1561 ttctcgatct cgtcgaacag cacgaccgaa tagggccggc ggcggaccgc ctcggtcaac
     1621 tggccgccct cttcgtagcc gacgtaacca ggcggcgccc ccatgagccg cgacaccgcg
     1681 aacttctcgg tgtactcgct catgtcgatg cgcacgatgg cgttctcgtc gtcgaacagg
     1741 aactcggcca gcgcgcgggc cagctcggtc ttcccgacgc ccgtcggccc caggaacagg
     1801 aacgagccca gcgggcggtt cgggtcctgt agcccggcgc gggcgcggcg caccgcgttc
     1861 gccacggcgg taatcgcctc gtcctggccc accacgcgca ggtgcaaccg ctcttccatg
     1921 cgcagcagct ttgccagttc gccttccagc atccgcgtca ccgggatacc ggtccacttg
     1981 ctgaccaccg cggcgatgtc atcctcggtc acttcctcgc gcagcagcgt gccgcccttg
     2041 cgcgggccgc tctccacctt ttgcgcggct tccaactggc gctgcagttc gggtaaccgg
     2101 ccatactgga tctcggcggc cttgttcagg tcgtagttgc gctgggcggc ttcgagttcg
     2161 gcgttggcct gttcgatctg ctccttcagc ccgcgcacag cctgctggcc ctggcgctcg
     2221 ctctggagtt gcgccgtcag ccggcgctcc tcttccttca ggttcgccag tgtgctctcg
     2281 atcttctgca gccggtcctt gctggcctcg tccttctcct tgctcagcgc ctgccgctcg
     2341 atctccaact gcatgacccg gcgcttcacc tggtccaact caaccggcat gctctcattt
     2401 tccatgcgca gccgcgaggc cgcctcgtcc accaggtcga tagccttgtc gggcaggaag
     2461 cggtcggtga tatagcggtt cgacagcacc gccgccgaga ctagcgcgct gtcggtgatg
     2521 cgcacgccgt ggtggacctc atagcgctca cgcaggccgc gcaggatcga gatcgtgtcc
     2581 tcgaccgtcg gttcgccgac gaagaccggc tggaagcgcc gctccagcgc ggcgtccttc
     2641 tcgatgtgct tgcgatactc gtccagcgtg gtcgcgccga ccatgtgcag ttcgccgcgc
     2701 gccagcatcg gcttgagcat gttggaggcg tccatcgcgc cctccgccgc gcccgccccg
     2761 accacggtgt gcaactcgtc gatgaagacg atgatctcgc cctcgctctc ggagatctcc
     2821 ttgagcaccg ccttcaggcg ctcctcaaac tcgccgcgga acttggcccc ggcgaccatg
     2881 gcgcccaggt ccagcgcgat cagtttcttg ttcttcaagc cttcgggcac gtcgccgcgc
     2941 acgatgcgct gcgccaggcc ctcgacgatg gccgtcttgc cgacgcccgg ctcgccgatg
     3001 agcaccgggt tgttcttggt gcgccgcgac agcacctgga tcacgcgccg gatctcctcg
     3061 tcgcggccga tgaccgggtc gagcttgccc ttctcggcct gcgcggtcag gtcgcgccca
     3121 tacttctcca gtgcggcata ggtcgcttcg gggttctggc tggtcacgcg ctggttgccg
     3181 cggatctggc tcagcacctg cagcacgcgg tcgcgggtga cgcctgccgc cttcagcagt
     3241 tgcgccgcgg gcgcgcgccc gccctcgtcg atcagcgcaa gcaacaggtg ctcggtgctg
     3301 acgaagtcgt ccttaatgtt tttggcctcg gtctcggcct tgttggcgac ggcgttgagc
     3361 cgggctgata cccgcggttc ggcggggccg ccgctcagcc gggccagccg gtcgatctcg
     3421 ctctgcgtct gctggatcag cggcgcgatg ggcacgttca gcttctgcaa gatcgcgggc
     3481 acgacgccgc cgtcctgctc cagcagggcc agcagcagat gctcgggttc gatctgcgtg
     3541 ttgttatgtt cggttgccag gctctgggcg gccaatacgg cctcctgcgc cttctccgta
     3601 tacttctgta aattcatggg ctacatcctc cgcaaagcgc ccctgcataa ctcgtgcccg
     3661 atagaaggcg gttatgcggg tggcgcctgg attgctattc aattgtcgat ggggttattt
     3721 gctgtactca gcacagctat ccactcttga tagttccatt atatcgggtg cctgttagaa
     3781 acacgcttgc cctgtgttag agccttgtta gagatgagtg gccctggatc aagcctgtag
     3841 aggcaggctt tgcgccgcat tgtcggcgag tacgcgggcc gggcagcgcg ggccgtggta
     3901 taatgccggt ggcgccgggt ggcgattcta ccggcgcggg gaaggtgcat gtgatggatg
     3961 ataccgagga taaggctata acgaccggga atgaggacgc cgtggcggaa ccggcgccag
     4021 gcgaggatgc cgaaccggtc gaagaagagg tcgatctcgc gaccattcaa gacgatccgg
     4081 aggcgttgca gcgcctcggc gaaagctacc tggaggccga ccgcaatgcc gaggccatcg
     4141 ctgtctacac ccggctgatc gagttgaagc ccgccgaccc cgagcccctg ctcacccgcc
     4201 tctgggcgca tatgaacgcc ggggatacgg aggcgacacg ggctgacgct gcgctgctgc
     4261 gcgaacgctt ccccgacgac gccgagatgc tggccaacct gggcgacgtg aacctgctcc
     4321 tggacgaccg cgaacaggcc ctgtatgacc tcgaccgcgc cgcgacgctg gcgggcgaca
     4381 acaacgagtt gctctaccgg gtggcgcgca gcttcgccat ggccgaggaa caggaaccgg
     4441 cgctggcgct ctatgaccgc ctggtcgagt ggaagccgcg cgaggtatt
//
LOCUS       DASUYC010000853         2549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_602633,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000853 DASUYC010000000
VERSION     DASUYC010000853.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602633"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            250..930
                     /locus_tag="VF276_14025"
     CDS             250..930
                     /locus_tag="VF276_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608018.1"
                     /translation="MTNTTAPPLPAFRAGDVILFAGQRDLYSRVARWIMRLPGEGPTY
                     SVHTAQFISARYVLEMDIVGRVKTVDDVLNKRSKLDMWQRRGFEVWRCRTLTRAQRQA
                     VARQTLAFRNLHFGGRAFLMHLFDGLVSQAARHDVYFFRRLNHRNRYPVCSEITAVAY
                     EAALDYQFGVPADCADPDQIYDWVAQHPDEWERVFCLAEFPQARQRSGGPAPAPAPER
                     ERELAQAR"
     gene            1032..2414
                     /locus_tag="VF276_14030"
     CDS             1032..2414
                     /locus_tag="VF276_14030"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEX6608019.1"
                     /translation="MSSLGRSLGTLLVLIAVLAGAPAARGGPLAPRPPATPLPATASP
                     CPPAWRLVAGPNPTWRYNQLRALAVVSAADVWAVGTASGRPLAAHWDGSAWSVAPMPN
                     TGLNTVTLYGAAALGPNDVWAVGSGYTANGDEQTLIVHWDGSAWRRVPSPNPGTYSNA
                     LSSVTAIAPDDVWAAGTSYTEGGAVTPILIVHWDGRAWSRVAALWVVPGDLRALAAVA
                     ADDIWAVGVVGEEPLTLHWDGTAWSQVFLPRLAGHTSLSSVAAIAADDVWAVGGYWNP
                     MNFRGGALIEHWDGAAWTQVPLLAASYPMISGLDGVTAVTAQDVWVGGATAGRIVMLH
                     WNGSAWSQVPGPETAGQFSVTGVAAVAAGDIWAIGGYNLYTGSDRTGLLHYTDTCGPP
                     APPPEGCVLPFTDVDAGHPFYSNIQCLYCSNILDGYSDGTFRPGASVTRGQTAKFISN
                     AFFPGCGPLR"
BASE COUNT          368 a          877 c          909 g          395 t
ORIGIN      
        1 gcgcgtgctg ggcgcgccgt gaggaggcgg gtggtcagtg gatggacggg cgttcaccgc
       61 gaaggaacga aggtaacgaa ggattttgca ggattctcag gagtattcgg cggaagaacg
      121 ggcgctcctt caatacaaat cacaaatccc aaaaattctt cgttgccttc gttccttcgc
      181 ggtgaaactc gtcgtaaatc cactgcccac tgcccactgc ccactgacca cggaaaggag
      241 caacgtacga tgacgaacac gaccgcgccg ccgctgcccg cgttccgggc gggggatgtt
      301 atcctgtttg ccgggcagcg cgacctctat agccgcgtgg cgcgctggat catgcgcctg
      361 cccggcgagg ggccgaccta ttcggtgcat accgcccagt tcatcagcgc gcgctacgtg
      421 ctggagatgg acatcgtcgg gcgggtcaag acggtggacg acgtgctcaa taagcgcagc
      481 aagctcgaca tgtggcagcg gcgggggttc gaggtctggc gctgccgcac gctcacgcgg
      541 gcgcagcggc aggcggtggc ccggcaaacg ctggccttcc gcaacctgca cttcggcggg
      601 cgggcgtttc tcatgcacct gttcgacggc ctggtcagcc aggccgcccg gcacgatgtc
      661 tacttcttcc gccggctcaa ccaccggaat cgctaccccg tctgctcgga gatcacggcg
      721 gtggcctacg aggccgcgct ggactaccag ttcggcgtcc cggcggactg cgccgacccc
      781 gaccagatct acgactgggt ggcgcagcat cccgacgagt gggagcgggt gttctgcctg
      841 gcggagttcc cgcaggcgcg gcagcgcagc ggcggcccgg ccccggcccc ggcgccggag
      901 cgggaacggg aactcgcgca ggcccggtag cgccatcccc gccgccggtg tatccgcagc
      961 gcaggctgcc ccggctgcgg ccccgtgcgg tcggggcggg gcgggcgggc ggatgacaca
     1021 ggagcgatag catgagtagt cttggacggt ccctggggac gctgctggtc ctgatcgcgg
     1081 tcctggccgg cgccccggcg gcgcggggcg gcccgctagc gccccggccc ccggcgaccc
     1141 cgctccctgc cacggcctcg ccgtgccccc cggcctggcg cctcgtcgcc ggccccaacc
     1201 ccacctggcg ctataaccaa ttgcgcgccc tggccgtggt aagcgccgct gatgtctggg
     1261 ccgtgggcac cgcctccggc cggcccctgg ccgcccattg ggacggcagc gcctggagcg
     1321 tggcgcccat gccgaacacc gggttgaaca ccgtcaccct ctacggcgcg gctgccctgg
     1381 gcccgaacga tgtctgggcc gtcggcagcg gctacacggc gaacggcgac gagcagacgc
     1441 tgatcgtgca ttgggacggc agcgcctggc gccgggtgcc cagccccaac cccggaacct
     1501 actcgaatgc cctctccagc gtgacggcca tcgcgccgga cgacgtctgg gccgccggca
     1561 cgagctacac agagggcggc gctgttactc ctatcctgat cgtgcattgg gacggccgcg
     1621 catggagccg cgtcgccgcg ctgtgggtgg tgccgggcga cctccgggcg ctggccgcgg
     1681 tggcggcgga cgacatctgg gccgtgggcg tggtcggcga ggaaccgctg accctgcatt
     1741 gggacggcac cgcctggagc caggtgttcc tgccccgcct ggccggccac acttcgctca
     1801 gcagcgtggc cgcgatcgcg gcggacgatg tctgggccgt cggtggttac tggaacccta
     1861 tgaactttcg cggcggggcg ctaatcgagc attgggacgg cgccgcctgg acgcaggttc
     1921 ctctcctcgc cgcctcctac cccatgatca gcggactcga cggggtcacg gcagtgacgg
     1981 cgcaggatgt ctgggtgggg ggcgcgacgg ccggcagaat cgtgatgttg cattggaacg
     2041 gcagcgcctg gagccaggtg ccggggccgg agaccgccgg ccagttctcg gtgacaggcg
     2101 tggcggcggt cgccgcgggc gatatctggg ccatcggcgg ctacaatctg tacaccggct
     2161 ctgaccggac gggcctgttg cattacacgg acacgtgcgg cccgcccgcc ccgccgccgg
     2221 agggctgcgt gctgccgttc accgatgtgg atgcgggcca ccccttctac agcaacatcc
     2281 aatgcctgta ttgctccaac atcctcgacg gctacagcga cggcaccttc cggcccggcg
     2341 cgagtgtgac gcggggccag accgccaagt ttatcagcaa cgcgttcttc cccggctgcg
     2401 gccccttgcg gtaggcaggg cggcaggccg ggggcacgcc gcgcaggagc ggtatgatga
     2461 ttcgtcaagt cagttcatgt ttgcgagcat tgggcagcct cgtcctggtg gccggcctgc
     2521 tggcggggtg cgggcaggcg gcggccccc
//
LOCUS       DASUYC010000854         2115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1452798, whole genome shotgun sequence.
ACCESSION   DASUYC010000854 DASUYC010000000
VERSION     DASUYC010000854.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1452798"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..357
                     /locus_tag="VF276_14035"
     CDS             127..357
                     /locus_tag="VF276_14035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608020.1"
                     /translation="MYPRRLNPEATLVPPGPPGPPAPPPLPQPTDLPPLPPDPIEPAN
                     PPVTPARPAPPTVPGEPEPVPAPVVPGVPVET"
     gene            complement(642..860)
                     /locus_tag="VF276_14040"
     CDS             complement(642..860)
                     /locus_tag="VF276_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608021.1"
                     /translation="MDQLVNEISTRTGITQDQARQAVQMAVDFVKQKLPPALSSQVDG
                     FLSGTNSGDMAQQAQQALGGLGDMFGKK"
     gene            complement(1029..1739)
                     /locus_tag="VF276_14045"
     CDS             complement(1029..1739)
                     /locus_tag="VF276_14045"
                     /inference="COORDINATES: protein motif:HMM:TIGR03717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX6608022.1"
                     /translation="MGDFIGAIFSIVLIDLVLSGDNALVIGMAVRNLPARQKRLAVLA
                     GGGGALLLRVILTALAAVLLRVPLIQAAGGLLLAWITYRLLRPSEESTEATGADNSFV
                     QALWTIIVADVSMSIDNVLAVGAAAEGDIPLLLFGLALSMGIIIAGGSLVSVMLGKLP
                     WLIYLGGIVLLILAGEMIAEDPVVADYLSHEVWWQWAISVGLGLIVALFLWAPWRKHG
                     TAAPDQRVAPRDTGAHLS"
BASE COUNT          377 a          785 c          655 g          298 t
ORIGIN      
        1 ggccgtcagt gaaatgctca taccgccgga ttatacacca cgcgccgccg gtccaacggc
       61 acgacttatg cgcgaggatc aaccggcgct atcatgcgcc gggagtgtag agaggaggac
      121 aaccctatgt atccgcgcag actcaaccca gaggcgacgc tggtgccgcc ggggccgcca
      181 gggcctccgg cccccccgcc gctgccccag cccaccgacc tgccgcccct gccgcccgac
      241 cctatcgagc cggccaatcc acccgtcacc ccggcccgcc cggcgccgcc cactgtgccc
      301 ggcgaacccg agccggtgcc cgcgcccgta gtgcccggcg tgccggtgga aacctgaagc
      361 catagcgttg aaccggggcc ggcggcgttt gtcgccggcc ccaccgcgtg ggccgggcac
      421 cgggcgcccg cagcgcgcta aaccgcccgc ctgcgcaaac gtcgcaagac tcgcgcaggc
      481 gggcggcgga attcgatacc ggttcgatag ggggtacgta caatggcggt gttctgctcg
      541 accaggacta ccagcccccg attaggccgg cgccgccgcc tctgagccaa cgcagtgcgg
      601 cctcgccacc aatcgggggc catctttgtg tccgccgggg gctacttctt gccgaacatg
      661 tcacccaggc cgccgagcgc ctgctgggcc tgctgggcca tgtccccgct gttcgtgccg
      721 ctcaagaagc cgtccacctg cgaggacagc gcgggaggca atttctgctt cacgaaatcc
      781 accgccatct gcactgcctg ccgcgcctga tcctgggtga ttccggtacg ggtcgagatt
      841 tcattcacga gctgatccat atgtctgcca tcctttcggt actgcgtaca tcgggccgtg
      901 gcgacgggcc gccccacggt gccggcgccc cctgcccgat ggctcggcca ggcagccgca
      961 acagcaatac ctgcaacgac gccgccccgg cccggtgacg cggcgttacg gcgcggatgc
     1021 agcatatgtc aggacaggtg ggcgccggta tcgcggggtg ccacccgctg atcaggggcg
     1081 gcggtgccat gtttgcgcca gggcgcccag aggaacagag ccacgatcag gcctaacccc
     1141 acgctgatcg cccattgcca ccagacctcg tggctcagat agtccgccac gaccgggtcc
     1201 tcggcgatca tctcgccggc gaggatgagc agcacgatgc cgccgaggta aatcaaccag
     1261 ggcagcttgc ccagcatcac cgagaccagg ctgcccccgg cgataatgat gcccatgctc
     1321 agggccaggc cgaagagcag cagcggaata tcgccctctg ccgccgcgcc caccgccagc
     1381 acattgtcga tgctcatcga tacgtcggcc acgatgatcg tccagagcgc ctgcacaaac
     1441 gagttatctg ctccggtggc ctcggtcgat tcctcggatg ggcgtagcaa gcgatacgta
     1501 atccaggcca ggagtagccc gcccgccgcc tggatcagcg gcacgcgcaa cagcacggcg
     1561 gccagcgcgg tcaagatcac ccgcagcagc agcgccccgc ccccgcccgc cagcaccgcc
     1621 aggcgcttct gccgggcagg caggttgcgc accgccatgc cgatcaccag cgcgttatcc
     1681 ccgctgagca cgaggtcgat cagcacaatg ctgaaaatcg ccccgataaa gtcccccata
     1741 cgctcaagaa gcccctttcc cgcgccaccg gcactaacca accattgcgc tgcaagatac
     1801 gggccacttg ccggcaagca ggccccgcgc cagcataccg cccccgcgcc gatcggtcaa
     1861 gggcagacac cgagatccgg caccgcacta acataagaat cttcgcgctc ttcgttcctt
     1921 cgcggtgaat tgattctcag atcaggcaga cagagcgaca gcccgcggac tggggagcga
     1981 ccgcgggctg ccggataagg aggttagggc cgttatacgg cgtccggggc ggcggtgggc
     2041 ctgccgcggc ccgtgctgcg ccgcgcccac aggagcaggc cggccaccag ggccgccagc
     2101 gccgcgctaa tggcc
//
LOCUS       DASUYC010000855         2013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1521772, whole genome shotgun sequence.
ACCESSION   DASUYC010000855 DASUYC010000000
VERSION     DASUYC010000855.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1521772"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VF276_14050"
     CDS             <1..259
                     /locus_tag="VF276_14050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608023.1"
                     /translation="EQVQSLMEQQGGVAASGSPTSILLIAKGCVGCFYIVFIGTITAG
                     LGALGGWLYGLRRPAPAAPPPPPPLPPMPPLPPVGPDGRAG"
     gene            272..859
                     /locus_tag="VF276_14055"
     CDS             272..859
                     /locus_tag="VF276_14055"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HEX6608024.1"
                     /translation="MDTLIQILAGFGLAAPAGLNAWLPLLIVGLAARFTNLIHLNAPY
                     NLLENGWVLLVLCVLLFIEIMADKVPIIDSANDAIHTFIRPAAGAILFAAQNDAVGGI
                     DPVLALILGLLAAGSVHAVKATARPVITASTAGIANPFVSLAEDAVAAVATIIALIVP
                     VVAAILVILFLILAIRLVLRWRRLRAARNNLQRAR"
     gene            849..1796
                     /locus_tag="VF276_14060"
     CDS             849..1796
                     /locus_tag="VF276_14060"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX6608025.1"
                     /translation="MRARRVAALLLLIVAAGCAGTPGPDDPLPTLPPLVQPLATRIPT
                     STPQPAPPAAQEAPAPADSPPAPGAVNPPPAAPAAPAGAPPDWTFSVVGDTWKDTPML
                     HYILQDAAAQGDVLQVNLGDLTPQGRPEQLADFARVIGGAALPVFPVPGNHDVAWSKT
                     TAPFEQYWPRHQSFDRQNAHFTLVDDSAVTLDQAELDWIDADLSATQQPVKFVFMHVP
                     ALMPYPVPAESALQAGGPEFMALMEKHHVTAVYAGHLHAYTRVEHNGIPYIITGGGGE
                     PLHVPSLLGGRYHYVRVAVRGDTSTDTVVLLNNVPTPGP"
BASE COUNT          273 a          754 c          664 g          322 t
ORIGIN      
        1 ggaacaggtg cagtcgctga tggaacagca gggcggcgtg gccgcgtccg gctcgccgac
       61 ctcgattctc ctgatcgcca aaggctgtgt cggctgcttc tacatcgtct ttatcggaac
      121 gattacggcg gggctgggcg cgctgggcgg ctggctgtat ggcctgcggc gcccggcgcc
      181 cgccgcaccg ccgcccccgc cgcccttgcc ccccatgccg ccgttgccgc cggtgggccc
      241 cgatgggcgc gcgggctaga ggaagtaccg tatggatacg ctgattcaga tcctggccgg
      301 gttcggtctg gcggcgcccg ccgggctgaa tgcctggctg ccgctgctca tcgtcggcct
      361 ggccgcgcgc ttcaccaacc tgatccatct caacgcgccc tacaacctgc tggagaacgg
      421 ctgggtgctg ttggtgctct gcgtgctgct cttcatcgag atcatggccg ataaggtgcc
      481 gatcatcgac tcggccaacg acgccatcca caccttcatc cgcccggcgg cgggggcgat
      541 cctctttgcc gcgcaaaatg acgccgtggg cggcatcgac ccggtgctgg cgctgatcct
      601 gggcctgctg gcggcgggga gtgttcacgc tgtcaaggcc acggcccgcc cggtgattac
      661 ggcgtcgacc gcgggcatcg ccaatccgtt cgtcagcctg gccgaagacg ccgtggccgc
      721 cgtcgccacc atcatcgccc tgatcgtgcc ggtggtggcc gcgatcctgg tgatcctgtt
      781 cctgatcctg gcgatccggc tggtgctgcg ctggcggcgg ctgcgcgcgg cccgcaacaa
      841 cctgcaacgt gcgcgctagg cgagtcgcgg ccctgctgct gctgatcgtg gcggcgggct
      901 gcgccgggac gcccggcccg gacgatcccc tgcccacgct cccgccgctg gtccagccgc
      961 tggcgactcg catccccact tcgacccccc agcccgcgcc gcccgccgcg caagaggcac
     1021 cggcgcccgc cgattccccg cccgcgcccg gcgcggtgaa ccctccgccc gccgcgcccg
     1081 cggcgcccgc cggcgccccg cccgactgga ccttctcggt cgtgggcgac acctggaagg
     1141 acacgcccat gctgcactac atcttgcagg atgcggcggc gcagggcgac gtgttgcagg
     1201 tgaacctggg cgacctgacg ccgcagggcc gccccgagca actggccgac ttcgcccgcg
     1261 tgatcggcgg cgccgcgctg cccgtctttc ccgtgcccgg caaccacgat gtcgcctggt
     1321 cgaagacgac cgcgcccttc gagcaatact ggccgcgcca ccagtcgttc gaccggcaga
     1381 acgctcactt cacgctggtg gacgacagcg ccgtgacgct cgaccaggct gaactggact
     1441 ggatcgacgc cgacctgagc gccacgcagc agccggtcaa gttcgtcttc atgcatgtgc
     1501 ccgcgctcat gccctacccg gtgcccgccg agtcggcgtt gcaggcgggc gggccggagt
     1561 tcatggcgct gatggagaag caccatgtca ccgccgtcta cgcgggccat ctgcacgcct
     1621 atacccgcgt cgagcacaac ggcatcccct acatcatcac cgggggcggc ggcgagccgc
     1681 tccatgtgcc ctcgctgctg ggcgggcgct accactacgt ccgtgtggcg gtgcgcggcg
     1741 acacttcaac cgacaccgtg gtcttgctga acaacgtgcc gacgccgggt ccctgatcgg
     1801 gccggtgttg cgccgcgtcg cgggcttgtg gtaagatgca gcgacaggga gacagatcat
     1861 ggccgcgcat gcaacttcaa ccgccgagac gcccgccgcg ggccggcacc agccgctcac
     1921 ccggctgcgg ccccgcgaca tcggcagcct cctcgaccag gcggtgatcc tctaccggca
     1981 caacttcatg acctgggtgg gcatcgtcgc ctt
//
LOCUS       DASUYC010000856         5542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_135270,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000856 DASUYC010000000
VERSION     DASUYC010000856.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5542
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135270"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="VF276_14065"
     CDS             complement(<1..988)
                     /locus_tag="VF276_14065"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX6608026.1"
                     /translation="MRYSLRLVPMVMLVLLSAVSLGPRAAAAPLLAGWQPTALATAHI
                     AGVTMVPAAPYTLYAAGDGVYRSDDGGVSWTPLTTAFSAAEVQVAPSNRQVLYAATGE
                     GCASGAPGTLWRSADGGATWQPRTGGPASLVVSATDAGDLLGIGCTGVYRSSDGGQHW
                     TLLPGSGVPNFDGHLLVGGRNDPAYLYAVFASEGGTPAIARSTDGGQTWQTQVLADWP
                     GPGDLAVDPQDARQVSFVSFAGYAVSTDAGATWTARNTGLPRAGTFYQLSTVAHDPAT
                     GTWYLGSGDPTQPRGVFQRRATPAWTAVAPAPGGEAISRLVFVPAGAGPALLA"
     gene            complement(1075..4005)
                     /locus_tag="VF276_14070"
     CDS             complement(1075..4005)
                     /locus_tag="VF276_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX6608027.1"
                     /translation="MADPQHDGQAAFHLDGLYCPECARAVEQRLRAQPHITRVHLDWP
                     HNQVHVGYHAALISPAEIAQIIAGTGCKCALAAPDPAQPAADHGAAPDPQRALQHLRH
                     GVDVQPITMGTKYDRMQYELPVTAAHAGHQPRPEARSDPHAGHTVVVAPADVAHPDPV
                     ANHAAHTETSPDHSGHPARIEHSPPESAAGPAMDHVTHTSADTAPGHAGHAAPAVDHT
                     GMDHTGHSEAMDHAAMGHGGMDHDMSDPGMAAAMEIDMRNKFFIALLLTIPIVLYSPV
                     GMSLTGLQLPTFGLDVNWILLVLTTPIVFYCGWIFIAGAYYSLRLRTLNMSVLIATGV
                     LAAYLASVLLTFLTAETFFEAAAMLVTFVLFGHWMEMRARKGTNDALRALFDLVPPQA
                     TVIRDGVEQTIPSSEVRVGDTVLLRPGDKVPVDGVVTQGSTTIDESLVTGESVPVAKR
                     PGDPVVAGAINRTGSVRFEATKVGADTVLAQIVELVRQAQNSKAPGQRLADRAAQYLV
                     IVAVGAGVLTFLAWYGIAGAGFLTALTFAISAVVIACPDALGLATPTAVAVGTGVGAR
                     HNILIKDAATLEGVSAVTAVVLDKTGTLTEGRPRVTDIVPAGGWSAEDLLRLVAAAEH
                     GSEHPLADAIITGARDRHLELAEAHQFQAIAGHGIEAQVEGHAILVGNRKLMTDRGIA
                     VDALDEQAATLAQAGRTPMFAAIDGRAAGLIAVADPIKPTAREAVRQLQERGIAVVMI
                     TGDNRHTADAVAAQLGIARVFAEVLPAQKAGYVKQLQAEGKRVAMVGDGVNDAPALAQ
                     ADIGIAIGAGTDVAIATAKVVLMKSDPLDVVRAITLSQATVRKMKQNLVWASIYNLLA
                     IPVAAGVFYPAFGIMLRPEWSALLMAMSSIIVAVNAVSLKRVEGDLARPAGVPVGPAP
                     QPAGGATGARGRAAPRWAMGATGMRRSWFDLQGYVALSQSHAWCRTGSAPSPVVVYCG
                     GSPL"
     gene            complement(4071..>5542)
                     /locus_tag="VF276_14075"
     CDS             complement(4071..>5542)
                     /locus_tag="VF276_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX6608028.1"
                     /translation="RLLPWLLTRIAFTRSRELFILAVVAVAVGTALGSAALFGVSLAL
                     GAFLAGVVLGESSISHQIGAEVLPFRDVFTVVFFVSVGMLVNPAYIVNNALQVLALTV
                     LIVLGKPLFTLLMGLVLPASGRTILVAALGRGQIGEFSFILGQAGVALAVLTQDQYSL
                     ILAGALISIVVNPLVFRLLPVVEGALKGMPALWRLLDRQGAIAALAPPALADHVVIVG
                     AGRVGEHILTVLERLGVPLLVVEIDAQRAAAFDRRGVATLFGDAANSEVLRHAGLERA
                     RVLVVTLPDEAATEMVVAAARHIAPPLPIVARAATRSGVGRLYTQGVDSVIQPELEGG
                     LEVVRHTLLALGFSADQVQPYTDAVRRDQYDIEVFTPQERQALDQMRNTVRGLEIVWR
                     RLEEGCPFVGQSLVEANVRARTGASVIAIIRDQQVLPNPKSGTRFEVGDLVGLIGDTA
                     QIAALDRLLAAPAPAPPGARPEAQAEHGDIPVQTYQQQL"
BASE COUNT          877 a         1969 c         1899 g          797 t
ORIGIN      
        1 cggccagcag cgccggccca gccccggccg gaacgaacac caaccggcta atggcctccc
       61 cgccgggcgc gggcgccacc gccgtccagg ccggggtggc gcggcgctgg aagacgccgc
      121 gcggctgggt gggatcgccg ctgcccagat accacgtgcc ggtggccggg tcgtgggcca
      181 ccgtgctcaa ctgatagaag gtgccggccc gtggtaggcc cgtgttgcgc gccgtccagg
      241 tcgcgcccgc atcggtggat acggcgtagc ccgcaaagct cacaaagctc acctgccgcg
      301 catcttgtgg atcgacggcg aggtcacccg gccccggcca atccgccagc acctgggtct
      361 gccaggtctg gccgccgtcg gtgctgcggg caatggccgg ggtgccgccc tccgaggcga
      421 agacggcgta gaggtaggcg gggtcattgc gcccgcccac cagcaggtgg ccgtcaaagt
      481 tgggcacgcc cgatccgggg aggagggtcc agtgctgccc gccgtcgctg ctgcggtaga
      541 cgcccgtaca tccgatgccc agcagatcgc cggcatccgt cgcgctgacg accagcgagg
      601 ccggcccccc ggtgcgcggc tgccaggtgg cgccgccatc cgcgctgcgc cagagtgtgc
      661 cgggcgcgcc gctcgcgcat ccctccccgg tggccgcgta gagaacctgg cggttggatg
      721 gggccacctg cacttccgcc gcggagaagg ccgtcgtgag cggggtccag gagaccccgc
      781 cgtcgtccga ccggtacacg ccgtccccgg cggcatacag ggtatagggc gcggcgggta
      841 ccatggtcac accggcgata tgcgcggtgg cgagcgccgt gggttgccac ccggccagga
      901 gcggggcggc ggcggcccgt ggccccaggc ttaccgcgct gaggaggacg agcatgacca
      961 tcgggaccaa tcggagtgag tatcgcatct gctgctcctt cctgatccgg cgggccaacc
     1021 cgcgattggc gccgcacggg cggacggttc cccaccgcgg gccggcgcat gttgtcaaag
     1081 agggctcccg ccgcagtata ccacaaccgg cgagggtgca gaaccggtgc ggcaccaggc
     1141 atgggattgc gacaaggcaa catatccctg aagatcaaac cacgagcgac gcatcccggt
     1201 agcccccatc gcccagcgcg gggcagcacg cccgcgcgct cccgtagccc cgcccgccgg
     1261 ctggggggcg gggcccacgg gcaccccggc gggccgggcc aggtcgccct ccacgcgctt
     1321 aagcgacacc gcgttgacgg ccacgatgat cgacgacatc gccatgagca gggcggacca
     1381 ctcgggccgc agcatgatgc cgaacgccgg gtagaacacg ccggccgcca ccgggatcgc
     1441 cagcaggttg tagatcgagg cccagaccag attctgcttc atcttgcgta ccgtggcctg
     1501 cgagagtgtg atcgcccgca ccacatcgag cgggtcgctc ttcatcagca cgaccttcgc
     1561 cgtcgctatc gcgacatccg tgcccgcacc aatcgcgatg ccgatatcgg cctgagccag
     1621 tgcgggcgcg tcgttcaccc cgtcgcccac catcgccacg cgcttgccct ccgcttgcag
     1681 ttgcttgaca taacccgcct tctgcgccgg caagacctcg gcgaacaccc gcgcaatgcc
     1741 aagctgcgcg gccacggcgt ccgcggtatg ccggttgtcg ccggttatca tcaccaccgc
     1801 gatgccccgc tcctgcaact gccgcaccgc ctcgcgcgcc gtcggcttga tcgggtcggc
     1861 caccgcgatc agccccgcgg cgcgcccgtc gatggcggcg aacatcgggg tgcgcccggc
     1921 ctgggccagg gtcgcggctt gttcgtccag ggcgtctacg gctatgccgc ggtccgtcat
     1981 cagcttacgg ttcccgacca ggatcgcgtg accctcgacc tgcgcttcaa taccgtgccc
     2041 ggcgatggcc tgaaactggt gcgcttcggc cagttccagg tggcgatcgc gcgcgccggt
     2101 gatgatggcg tcggccagcg ggtgctcgga gccgtgctcg gccgccgcca ccagccgcag
     2161 caggtcctcg gcgctccagc cgcccgccgg cacgatatcg gtgacgcggg ggcggccctc
     2221 ggtcagcgtg ccggtcttgt cgagcaccac cgccgtgacc gcggagactc cttccagcgt
     2281 cgcggcgtcc ttgatcagga tgttgtgccg ggcgcccacg cccgtaccga ccgcgacggc
     2341 cgtcggcgtg gccagcccga gcgcgtcggg gcaggcgatg acgacggcgg agatggcgaa
     2401 ggtcagcgcc gtcaggaagc cggcgcccgc gatgccgtac caggccagga aggtgagcac
     2461 cccggcgccg acggccacga tcaccagata ctgggccgcc cggtcggcca gccgctggcc
     2521 gggcgcctta gagttctgcg cttgccgcac cagctcgaca atctgcgcca gcacggtgtc
     2581 ggcgccgact ttggtggcct cgaagcgcac actgcccgta cggttgatcg cccccgccac
     2641 cacggggtcg cccggccgct tggcaaccgg gacgctctcc cccgtgacca gcgattcgtc
     2701 aatggtggtg ctcccctggg tcacaacacc atccaccggc accttgtcgc cggggcgtaa
     2761 cagcaccgta tcgcccaccc gcacctcgct gctggggatc gtctgctcga cgccgtcccg
     2821 gatcaccgtc gcctgcggcg gcaccaggtc gaacagagcg cgcagcgcgt cgttggtgcc
     2881 cttgcgggcg cgcatctcca tccagtgccc gaagagcacg aacgtcacca gcatggccgc
     2941 cgcctcaaag aaggtctcag cggtcaggaa ggtcaacagc acgctggcta aataggcggc
     3001 cagcacgccg gtggcgatca gcacgctcat gttgagcgtg cgcaggcgca gcgaatagta
     3061 ggcgcccgcg atgaagatcc agccgcaata gaagacgatc ggcgtggtca agaccagcag
     3121 gatccagttc acgtcgaggc cgaaggtcgg gagctgtagg ccggtgaggc tcatgccgac
     3181 cggcgagtag agcacaatgg ggatcgtgag cagcagcgcg atgaagaact tgttgcgcat
     3241 gtcgatctcc atcgccgcgg ccatgccggg gtcggacata tcgtggtcca tcccgccgtg
     3301 ccccatcgcg gcatggtcca tcgcctcgct gtgcccggta tgatccatgc cggtatggtc
     3361 caccgcgggc gcggcatgcc cggcgtggcc cggcgccgtg tctgcgctgg tatgtgttac
     3421 atgatccatc gccgggcctg cggcagactc aggcgggctg tgctctatgc gcgccggatg
     3481 cccggagtgg tcggggctgg tctctgtatg cgctgcgtga tttgccactg ggtcgggatg
     3541 agcaacatcc gcgggggcaa caaccacggt gtggccggcg tggggatcgc tgcgtgcctc
     3601 cgggcgaggc tggtgcccgg cgtgcgctgc tgtcaccggc agttcgtatt gcatgcgatc
     3661 gtatttggtg cccatcgtga tcggctgcac gtccacgccg tgcctgaggt gttgcagggc
     3721 acgctgcggg tccggcgccg ccccgtggtc cgcggcaggc tgggcgggat cgggtgcagc
     3781 tagcgcgcat ttgcagccgg tgccggcgat gatctgcgcg atctcggccg gcgagatgag
     3841 ggcggcgtga tacccgacat ggacctggtt gtgcggccag tcgaggtgca cacgggtgat
     3901 gtgcggctgg gcgcgcaggc gctgttcgac ggcgcgcgcg cactccgggc agtatagccc
     3961 gtccaggtga aacgcggcct gcccgtcgtg ctggggatct gccatcgtct acctcctatg
     4021 cgctgctgca aacacgccat gaaccacggc gacggcagcc gcacgccggt ttacaattgc
     4081 tgctgatagg tttgcactgg gatatcgccg tgctccgctt gcgcctctgg tcgtgcgccg
     4141 ggcggggcgg gcgccggggc ggccaggagt cgatccagcg ccgcgatctg cgcggtgtcc
     4201 ccgatcaggc ccaccaggtc gcccacctcg aagcgggtgc ccgatttggg attgggcagc
     4261 acctgttgat cgcggatgat ggcgatgaca gatgcgccgg ttcgcgcgcg gacgttcgct
     4321 tccacgagcg actggcccac gaacgggcag ccctcctcca gccggcgcca cacgatctcc
     4381 aggccccgca ccgtgttgcg catctgatcg agggcctgcc gctcctgcgg cgtgaagact
     4441 tcaatgtcat actgatcgcg gcggaccgca tcggtatagg gctgcacctg gtcggcggag
     4501 aagcccagcg ccagcaaagt gtggcgcacc acctccaggc ccccctccag ttccggctgg
     4561 atcacgctgt ccacgccctg agtgtacagg cgccccacgc ccgaccgcgt ggcggcgcgg
     4621 gcaacgatcg gcaggggcgg ggcgatatgc cgggcggcgg ccacgaccat ctccgtggcc
     4681 gcctcatcgg ggagggtgac gaccagcacg cgcgcccgct ccagccccgc gtgccgcagc
     4741 acttccgaat tggcggcatc cccaaacaga gtcgccactc cgcgccggtc gaacgcggcg
     4801 gcccgttgcg cgtcgatctc gaccaccagc agcggcacgc ccagccgctc cagcaccgtc
     4861 aggatgtgct cgcccacccg gcccgcgccg acgatcacga catgatcggc cagggccggc
     4921 ggggccagcg cggcgatggc cccctggcga tcgaggagcc gccagagggc gggcatccct
     4981 ttgagcgccc cttcgaccac cgggagcagc cggaacacca acgggttcac aacaatcgag
     5041 atcaacgctc cggcgagaat cagcgaatac tgatcctgcg tcagcactgc cagggccacg
     5101 cccgcctgcc ccaggatgaa ggagaattca ccgatctggc cccggcccag cgcggccacc
     5161 aggatcgtcc gccccgacgc gggcagcacg agccccataa gcagcgtgaa cagcggcttg
     5221 ccgagcacga tcagcaccgt cagggcgagc acctggaggg cgttgttcac tatatacgcc
     5281 gggttgacca gcatcccgac cgagacgaag aacaccacag taaagacatc gcgaaacggc
     5341 aacacctccg cgccgatctg gtggctgatg gaggattcgc ccagcacgac gcccgccagg
     5401 aacgcgccca gggccagcga cacgccgaac agggcggcgg atcccagcgc ggtgcccacg
     5461 gctaccgcca ccacggccag gatgaacagt tcgcgcgagc gggtgaaggc gatccgcgtc
     5521 agcagccagg gcaacagccg ca
//
LOCUS       DASUYC010000857         3948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_135287,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000857 DASUYC010000000
VERSION     DASUYC010000857.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135287"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /gene="rimM"
                     /locus_tag="VF276_14080"
     CDS             <1..386
                     /gene="rimM"
                     /locus_tag="VF276_14080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX6608029.1"
                     /translation="EDAARTGPWAGQIHQALIKLAGVNDPETGDKLRGYLLQVPASEA
                     WPLPPDTFYAFQLYDLPVLTPDDVKVGVVEDLLATGSNDVYVVRTVAGKELLVPAVKD
                     IVLEINPAGGYIRIKDPAEWSDDED"
     gene            373..1719
                     /locus_tag="VF276_14085"
     CDS             373..1719
                     /locus_tag="VF276_14085"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6608030.1"
                     /translation="MTKTSLPRAPLSRVLRRPPGFKVLPAVPEQPSNSLLPSQIRHGM
                     RMSIGDGVFAQVYISITAGSFVTALALYMGASDFVLGLITAMPVLAQLVQLPAAWMVE
                     RRGDRRSITVWSSLGRLFWLVPVLLLYVPMDPTARLGIALLAMALAFGLLAISTNAWL
                     SWMSDLIPPSLRGRFLSVRSMVLAIIGLGVSYVGGTLLDAARKAGHAEWGFLGLYTVA
                     CAAGLVSTYFVSRQPEPPFARAAGGTSFRKLVQAPWRDRNFRAFIFTLTVWSIGINIA
                     APFYNAQALKNLHVSYQQLATLDMTTIAVTLLTQPFWGRWSDRVGHRRTLMISMIGAS
                     PLALMWLIATPSTIWLIYLNNVLSGIFWPGVNLSLSNRLMERAPAAGRAGYLALYSAI
                     TGGLAFLASMFGGLIAGGLEGHYFQFGPLGLNNYQVLFLMAGVIRISVALLRSRSV"
     gene            complement(1802..>3948)
                     /gene="glgB"
                     /locus_tag="VF276_14090"
     CDS             complement(1802..>3948)
                     /gene="glgB"
                     /locus_tag="VF276_14090"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017662135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEX6608031.1"
                     /translation="GEGRHYRTYEKLGAHVCEAGGVRGVHFAVWAPNALRVSVVGNFN
                     NWDGRVYPMRFHAGQGIWEIFLPGLGPGEAYKYEIKSRFNDYLVEKADPYGFYSELRP
                     RTASVVADLTRYSWGDADWMSNRAANNGQDAPIAIYEVHLGSWQRVPEEGNRWPTYRE
                     LAHQLVDYVKYMGFTHIELMPVSEHPFDGSWGYQTTGYYAVTSRYGTPEDFMYFVDRC
                     HQAGIGVFLDWVPAHFPKDISGLNYFDGTHLYEHSDPRLGEHPDWGTLVFNFGRNEVR
                     NFLLSNALFWLDKYHIDGLRVDAVASLLYLDFSRQEGAWVPNKFGGRENLEAIDFLKR
                     FNELVHLEHPDVLTMAEDSTAWPMVTKPTFLGGLGFDFKWNMGWMHDMLDYLSYNPIY
                     RRYHHNLITFSLMYAFSENFLLPLSHDEVVHLKSSLLNKMPGDGWQQFANLRTLLGYM
                     FTHPGKKLLFMGGEFGQRSEWSEARSLDWHELNNESHQKLHRYVHDLVHLYNSEPALW
                     QIDNSWEGFQWMAANDNENSVISFTRRGTDPNDVILVVCNFTPVPRYNYCIPAPRAGY
                     YQEILNSDSESYWGSNLGNMGGTEAYPQDWSSTGYAICLTLPPLSTLLFKASPLPPAA
                     ATEEAVALEAAAETPSEVDALNAPVQGQETVSTTVIEENAASPPTKRTRKKVAPPEEA
                     VTEPAVPSEPAPGPEAAPAPRKRTRKTTEGAQ"
BASE COUNT          632 a         1236 c         1305 g          775 t
ORIGIN      
        1 ccgaggatgc tgcgcggacc ggcccctggg ccgggcagat ccaccaggcg ctgatcaagc
       61 tggccggcgt gaacgacccg gagacgggag acaagctgcg cggctacctg ctgcaagtgc
      121 ccgcgagcga agcctggccc ctgccgcccg atacttttta cgcgtttcag ctatatgatc
      181 taccagtgct cactcccgat gatgtgaagg tgggtgtcgt cgaggatttg ctcgcgactg
      241 gcagtaatga tgtgtatgtg gtccgcaccg tcgcgggcaa agagcttctg gtgccggcgg
      301 tgaaggacat cgtgcttgag atcaacccgg cgggaggcta tatccggatc aaggacccgg
      361 ccgagtggag tgatgacgaa gactagcctg ccgcgggcac ccttaagccg cgtgctgcgg
      421 cgtccccccg gttttaaagt tctacccgcc gttcccgagc aaccgtccaa cagcctgcta
      481 ccctctcaga tccgccacgg catgcgcatg tcgattggcg acggcgtctt tgcccaggtc
      541 tacatcagca tcaccgccgg ctcgtttgtg accgcgctcg ccttgtacat gggcgccagc
      601 gacttcgtgc tagggctcat cacggccatg ccggtgctgg cgcagttagt ccaactgccc
      661 gcggcctgga tggtcgagcg gcgcggcgac cgccggtcca ttaccgtctg gtccagcctg
      721 ggccggctgt tctggctggt gccggtgctg ctgctgtatg tgccgatgga cccgaccgcg
      781 cgcctgggca tcgccctgct ggcgatggcg ctggccttcg ggctcctggc gatcagcacc
      841 aatgcctggc tgtcatggat gagcgatctc attccgccct cgctgcgcgg gcgcttcttg
      901 agcgtgcgta gcatggtcct cgccatcatt ggcctgggcg tgagctatgt gggcggcacg
      961 ctcctggacg cggcgcgcaa ggccggccat gccgaatggg gcttcctggg cctgtacacc
     1021 gtcgcctgcg ccgcggggct ggtcagcacc tatttcgtca gccggcagcc cgagccgccg
     1081 ttcgcgcggg ccgccggggg caccagcttc cgcaagctgg tgcaggcgcc ctggcgcgac
     1141 cgcaatttcc gtgcgtttat cttcacgctg accgtctgga gcatcgggat caatatcgcc
     1201 gcgcccttct ataacgccca ggcgctcaag aacctgcacg tgagctacca gcaactcgcc
     1261 acgctcgata tgacgaccat cgccgtgacg ctgctgacgc agccgttctg gggccgctgg
     1321 tccgaccggg tgggccaccg gcgcacgctg atgatcagca tgatcggcgc gtcgccgctg
     1381 gcgctgatgt ggcttatcgc cacgccctcg acgatctggc tgatctacct caacaacgtg
     1441 ctgtcgggca ttttctggcc gggggtgaac ctgtcgctaa gcaaccgcct gatggagcgg
     1501 gcgcccgccg ccgggcgggc cggctacctg gcgctctact cggcgatcac cggcggcctg
     1561 gcctttctcg cctccatgtt tggcgggctg atcgccgggg gactggaagg ccactacttc
     1621 caattcgggc cgctgggcct gaacaactac caggtgctct tccttatggc cggggtgatt
     1681 cgcatcagcg tggcgctctt gcgcagccgt agcgtgtagc cccgcgcggc ggggagtagg
     1741 tctcgaaaga aaaagcacgc cacatttgat cggtggcgtg ctttttcttt gacaggcccg
     1801 gttactgcgc gccttccgtc gtcttgcggg tgcgcttgcg cggggcgggc gctgcctccg
     1861 gtcccggcgc gggttccgac ggaaccgccg gctcggtgac ggcctcctcc ggcggggcga
     1921 cttttttgcg cgtgcgcttg gtgggcggac tcgccgcgtt ttcctcgatc accgtggtgg
     1981 agacggtttc ctggccctgg accggcgcgt tcagcgcatc gacctcgctc ggcgtctcgg
     2041 cggccgcttc cagtgccacg gcctcctctg tggcggcggc gggcggcagc ggtgatgctt
     2101 tgaacagcag cgtgctcagg ggcggcagag tcaggcagat ggcgtagccc gtgctcgacc
     2161 agtcctgcgg gtacgcctcg gtcccgccca tgttgcccag gttgctgccc cagtaggact
     2221 cggagtcgct gttcaggatc tcctggtagt agcccgcgcg gggcgccggg atgcagtagt
     2281 tgtagcgcgg cacgggcgtg aagttgcaga cgaccaggat cacgtcgttg gggtcggtcc
     2341 cccggcgcgt gaaggagata acgctgttct cgttgtcgtt ggccgccatc cactggaaac
     2401 cttcccagct attatcgatc tgccagagcg ccggttcgct gttgtagaga tgcaccaggt
     2461 catggacgta gcggtgcagc ttctggtgcg attcgttgtt gagttcatgc cagtcgaggc
     2521 tgcgcgcttc gctccactcg ctgcgctggc cgaactcgcc gcccatgaac agcagtttct
     2581 tgccggggtg ggtgaacata tagcccagca gcgtgcgcag gttggcgaac tgctgccagc
     2641 cgtcgcccgg catcttgttc agcagcgagc tcttcagatg caccacctcg tcatgcgaca
     2701 gcggcagcag aaagttctcg ctgaaggcgt acatcagcga gaacgtgatc aggttgtgat
     2761 ggtagcgccg gtagatcggg ttataggaca ggtagtcgag catgtcgtgc atccagccca
     2821 tgttccactt gaaatcgaag cccaggccgc ccaggaacgt cggcttggtc accatgggcc
     2881 acgcggtgga gtcttcggcc atggtcagca cgtcggggtg ctccaggtgg accagttcgt
     2941 tgaagcgctt gaggaagtcg atggcctcca ggttttcgcg gcctccaaac ttgttcggca
     3001 cccaggcacc ctcctggcgc gagaaatcga ggtagagcag cgaggccacg gcgtccaccc
     3061 gcaggccgtc gatatgatac ttgtcgagcc agaacagcgc gttcgacagc aggaagttgc
     3121 gcacttcgtt gcggccgaag ttgaacacca gcgtgcccca gtcggggtgt tcgcccaggc
     3181 gggggtcgga atgttcgtag aggtgggtgc cgtcgaagta gttcagcccg gagatgtcct
     3241 tcgggaagtg ggcgggcacc cagtcgagga acacgccaat ccccgcctgg tggcagcgat
     3301 cgacaaagta cataaagtct tcgggcgtgc cgtagcggct ggtgacggcg taatagccgg
     3361 tggtttgata gccccaggag ccgtcaaacg ggtgttccga cacaggcatc aactcaatat
     3421 gggtgaagcc catatatttg acataatcta caagctggtg ggctagttcc cggtaggtgg
     3481 gccagcggtt gccttcctcc ggaacccgct gccaggaacc gaggtggacc tcataaatgg
     3541 cgatgggggc atcctggccg ttgttcgcgg cgcggttgct catccagtcc gcgtcgcccc
     3601 agctataacg ggtcaggtcg gcgacgaccg atgcggtgcg cgggcgcaac tcgctataga
     3661 agccgtaagg atcggccttc tcgacgagat aatcgttgaa gcgcgacttg atctcgtact
     3721 tatacgcctc gcccggtccc aggcccggca agaaaatctc ccagatgccc tggccggcgt
     3781 ggaagcgcat cgggtagacg cgcccgtccc agttgttgaa gttgccgacc acgctgacgc
     3841 gcaaggcatt aggcgcccag acggcgaaat gcacgccgcg cacgccgccg gcttcgcaca
     3901 catgggcgcc gagtttctcg taggtgcggt agtgcctgcc ttcgccga
//
LOCUS       DASUYC010000858         3819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1752529, whole genome shotgun sequence.
ACCESSION   DASUYC010000858 DASUYC010000000
VERSION     DASUYC010000858.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1752529"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VF276_14095"
     CDS             complement(<1..228)
                     /locus_tag="VF276_14095"
                     /inference="COORDINATES: protein motif:HMM:NF014586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608032.1"
                     /translation="MASLTNAAAGSAAPGQPIELVPAWPAGLDERLARMRELVAGFGR
                     TLVAYSGGVDSAVVLKICRDVLGKDQVLGVLA"
     gene            498..851
                     /locus_tag="VF276_14100"
     CDS             498..851
                     /locus_tag="VF276_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608033.1"
                     /translation="MTNTPDGNRLPETPVGMRSRPSDVVANPPVVKTPVPEGAVPAVP
                     GAGVRPGAAPADSAALAAERAARVADIVTDEPHGLTTDQIMPTCMIYSMFAVLAVLLG
                     TILCWMINAFGMLIR"
     gene            complement(929..1417)
                     /locus_tag="VF276_14105"
     CDS             complement(929..1417)
                     /locus_tag="VF276_14105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608034.1"
                     /translation="MDEPIRVRCLACQARLELPPRATYAACRTCGSEYLVSRRGGAYA
                     LQPLAPEEVALTQQVAAVEREQEMGCANTALSLLAGIVILFCVVGGLGQVLFNNRLVC
                     VGSWVVALALVVPGAIVMLRNLQRDGQRKARLLAQPPAASEAPAPPGNGPDAPAPEST
                     DP"
     gene            1781..2419
                     /locus_tag="VF276_14110"
     CDS             1781..2419
                     /locus_tag="VF276_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX6608035.1"
                     /translation="MGFNIEQLIETAGYLGLFAIIFAESGLFFGFFLPGDSLLLTAGL
                     LAWRGHLDLNVLLPLLFAAAVLGDNVGYWFGKKVGPPLFNREASLLFRRKNLLAAEAF
                     YAKHGGKAIVLARFAPFIRTFVPIVAGAVQMDYRRFFLFNVLGGFLWTIGLTVLGFVI
                     GNAFGHVEGVSKYIEIFILGVIAVSLLPAVIHAWREYRHEVFAAVRARFDGA"
     gene            complement(2546..>3819)
                     /locus_tag="VF276_14115"
     CDS             complement(2546..>3819)
                     /locus_tag="VF276_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608036.1"
                     /translation="AEKELSTLGLRADVIAHARKNPLSPPPQSKKGPAEQQREYQEAE
                     AWYEASTRYGDMSAKLSEMQKEYSAAKATKETDPERYKAAWVHYTQAYKLYQSTAVEA
                     DAWAVGGMVESTLGFKPNTLETELAKMPADPRTFTPIGGATSTPQPPMRPRAVATGAP
                     RAYNPQPDNISTSASPLTPPVRQARKLGGFSPLTPLDPGTIGAIETQVPGQDDSTAAD
                     TSSSPGAITTSALPLTPPVRTPRKLGGFSPLTPLDPSTIGAIETQVPDQDESPSGGSI
                     STAPPQIISPSNGPRRPRLGVFSPRPALNPSTSNMSGPETADLAAPTLAGSATSGGAI
                     STDAAPLEPPVRRGRKLGAFSPLNPLDPSTISAADTPDVTPTPNPTGGPRRDDDETNL
                     QLTVQRHAMPEDLAQALSREQIPQEELTAKG"
BASE COUNT          572 a         1312 c         1240 g          695 t
ORIGIN      
        1 ggccagcacg cccagcacct ggtctttgcc cagcacatcg cggcagatct tcagcaccac
       61 cgcgctgtct acgccgcccg aataggcgac cagcgtgcgc ccgaacccgg cgacaagctc
      121 ccgcatccgc gccaggcgct cgtcgaggcc ggcgggccag gcgggcacta actcaatcgg
      181 ttggccgggc gccgcgctgc ccgcggcggc attcgtaagg gaagccaaag cagtaactcc
      241 gtcctttccg gcatgcggcg gcccgcatgc cacctgttct caacgctgta gtataccaca
      301 ccgcacggtg cgttccaccg tgcctccggc tgaacatcct ccatacgcac cgtcgaagcg
      361 ccgaggacgc ggattagcgc gctctatagc cattgtgcca aacgcccttc tgctgtataa
      421 tggcggcgtt ctgtgcgcaa aagcacaacc ccgcgccatc gcacgccggg cacgcacgag
      481 gaggagacgc tggacccatg actaatactc ccgacggcaa caggcttccc gaaacgccgg
      541 tgggtatgcg cagccgccca tcggatgtgg tggcaaaccc gccggtcgtg aaaactccgg
      601 tccctgaagg cgcggtgccc gcggtgccgg gcgccggggt gcggcccggc gcggccccgg
      661 cggattccgc ggccctggcc gccgagcgcg cggcgcgcgt ggccgacatc gtcaccgacg
      721 aaccgcacgg cctgaccacc gaccagatta tgccgacgtg catgatctat tcgatgttcg
      781 ccgtactcgc cgtgctcctg ggaacgatcc tctgctggat gatcaacgcc ttcggcatgc
      841 tgatccgcta aggttcccct acaccacata cgtatgcagg cgggccggat cgacccgcct
      901 gcgtttcgcc tgggttcgac cgaccgggct atgggtccgt cgattccggg gcgggcgcgt
      961 ccggcccgtt gccgggtggc gcgggcgctt cggatgctgc cggcggctgg gccagcaggc
     1021 gcgccttgcg ctgcccgtct cgctggaggt tgcgcaacat cacaatcgcc cccggcacca
     1081 ccagggccag cgcgaccacc cacgagccca cgcagaccag gcgattattg aacagcacct
     1141 gccccagccc cccgaccacg cagaatagaa tgacgatccc ggccagcagc gagagcgccg
     1201 tattggcgca gcccatctcc tgctcgcgct cgaccgccgc cacctgctgc gtcagcgcca
     1261 cctcctccgg cgccaggggt tgcagcgcat acgccccgcc gcgccggctc accaggtatt
     1321 cgctgccgca cgtgcgacag gcggcatagg tcgcccgcgg cggcagttcc agacgcgcct
     1381 ggcaggccag gcagcgcacg cgtatcggct catccatacc cgcattatag ccgaagcgcc
     1441 cggccccccg ccacgcgccc cgaccctcaa gggtcgggct acagctataa agcccgcctc
     1501 cgcgggctgc tttccatgag ggaccaggcc gcgcaggcgg ccttcgtcat ggtagccgga
     1561 ggcttgagcc tccgggcgcg cccagcctga gcgttcgata agaaaaggcc cgccgggctt
     1621 gaaccggcgc cggacgcctt ggtacaatgg tgattgtggc ggaccatgca cacaacaacc
     1681 ggcatggccc gttctttatt gatactgcaa aatcaccaac cattccaccc ccgccgccgg
     1741 accgggccgg ggcaccacac ggaggacgat tcatcacacg atggggttta atatcgaaca
     1801 actcattgaa accgccggct atctcggcct gtttgccatc atcttcgccg aatccgggct
     1861 gttcttcggc ttctttttgc cgggcgactc gctgctgctg accgccgggc tgctggcctg
     1921 gcgcggccac ctcgacctca acgtgctgct cccgctgctg ttcgccgccg ccgtgctggg
     1981 cgacaatgtg ggctactggt tcggcaagaa ggtcgggccg ccgctgttca accgcgaggc
     2041 gtcgctgctg ttccggcgca agaacctgct cgccgccgaa gccttctacg ccaaacacgg
     2101 tggcaaggcc atcgtcctgg cgcgctttgc cccgttcatc cgcaccttcg tgccgattgt
     2161 cgcgggcgcc gtccagatgg actatcgccg cttcttcctg ttcaacgtgc tgggcggctt
     2221 cctctggacc atcggcctga ccgtcctggg cttcgtcatc ggcaacgcct tcggccatgt
     2281 cgagggcgtc agcaagtaca tcgagatctt catcctgggc gtcatcgccg tctcgctgct
     2341 cccggcggta atccacgcct ggagggaata ccggcacgaa gtcttcgccg ccgtgcgcgc
     2401 ccgcttcgac ggggcgtaag ctcgcccggc tcccgcactc cccgatccac acatagacgg
     2461 cgcccgacct tgcggtgggc gccgtccgcg tattgccggg gatcgcgtct ccggtgattc
     2521 ccagcagatc gtgtgtaacc ccggattaac ctttcgcggt tagctcctcc tgcgggatct
     2581 gctcgcggct gagcgcctgg gccaggtcct cgggcatggc gtggcgctgc accgttagct
     2641 gtagattcgt ttcgtcgtcg tcgcgccggg ggccgccggt agggtttggg gtgggcgtta
     2701 catccggtgt gtcggccgcg ctgatagtgc tggggtcgag ggggttcagc gggctaaatg
     2761 cacccagttt gcgaccgcgc cggaccggcg gctcgagcgg cgccgcatcc gtgctgatcg
     2821 cgccgcccga agtcgcgctg ccggcgaggg tcggggcagc cagatctgcg gtctcggggc
     2881 cgctcatgtt gctggtactc gggttgaggg cgggccgcgg gctgaataca cccagccgcg
     2941 ggcggcgtgg accgttggat ggcgatatga tctgcggcgg tgccgtggag atcgagccgc
     3001 ccgacggcga ttcgtcctga tcggggacct gggtctcaat cgcgccgatg gtactcgggt
     3061 cgaggggggt cagcgggctg aagccaccca gtttgcgcgg ggtccgcacc ggcggcgtga
     3121 gcggcaatgc agatgtagtg atcgcaccgg gcgaactgct ggtatcggcg gcggtcgaat
     3181 cgtcctgacc cggaacctgc gtctcaatcg cgccgatcgt ccccggatcg aggggggtca
     3241 gcgggctgaa gccaccgagc ttgcgggcct gccgcaccgg cggcgtgagt ggcgacgcag
     3301 aggtgctgat gttgtcgggc tgcgggttgt aagcccgcgg ggcaccagtc gccaccgcgc
     3361 gcgggcgcat gggcggctgc ggcgtgctgg tggcgccgcc gatgggcgtg aaggtccgtg
     3421 gatcggcagg cattttcgcc agctcggttt ccaacgtgtt cggtttgaat cctagcgtgg
     3481 attctaccat cccacccacg gcccaggcgt cggcttcgac cgcggtagac tggtagagtt
     3541 tataggcttg ggtgtagtgt acccaggcgg ccttatacct ttccggatca gtctctttgg
     3601 tggccttcgc ggcggagtat tctttttgca tttcacttag tttggcactc atatcgccat
     3661 agcgcgtgct ggcttcgtac caggcttccg cttcctgata ctcgcgctgt tgctccgccg
     3721 gccctttctt cgactgcggc gggggcgata acgggttttt tcgcgcgtgg gcaatcacgt
     3781 cggcgcgcaa gccaagagtg ctcagctcct tttctgccc
//
LOCUS       DASUYC010000859         3079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_597777,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000859 DASUYC010000000
VERSION     DASUYC010000859.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597777"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(346..1416)
                     /gene="selD"
                     /locus_tag="VF276_14120"
     CDS             complement(346..1416)
                     /gene="selD"
                     /locus_tag="VF276_14120"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(1360..1362),aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEX6608037.1"
                     /translation="MDDNRAIVRLTSLATCAGUAGKMGPGDLAHVLRHLTPHTHPALL
                     VGLHTSDDAAVYQLNADQALVQTLDFFPPIVDDPYLFGAIAATNALSDVYAMGGHPIL
                     ALNIAMWPEDLDRALLAAVLQGGADKVAEAGAIVAGGHTVTDREPKYGLSVTGIVHPA
                     HLLTKGGAQPNDVLVLTKPVGTGVITTAARADRARPADLAGAVASMTELNRAASEALI
                     ACGVHAATDITGFGLLGHAYEMAEAGGVQLVIEADEVPLLAGAWDYAEAGLTTGGGGR
                     NYAYLMEPDSAAGLTQPRVHVRRPVPDSLQALLLDPQTSGGLLAAVPPLEYGELSARL
                     GDAGVLVYRIGEVVEGRGVDVT"
     gene            complement(1451..2782)
                     /locus_tag="VF276_14125"
     CDS             complement(1451..2782)
                     /locus_tag="VF276_14125"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX6608038.1"
                     /translation="MSSTSEFTSRPGAAIRRAFVPALVLAALIAAPGCTLSNLRPEAG
                     ATPTAAAVVTPTAPAQAATPTPNAIPTATTGAPATPTTGSAFATPTTGSGVTTPTTGT
                     GTPTPSGSSAPATATTGTSPLTGIPSAAEMFERVVDAVGPAVVTVVNKLDPQQGFGGE
                     ASGSGVVIDAKGYIVTNNHVVANNSALQVIFSDGRTVNATLVGSDDISDLAVLKVDGT
                     VPGVATLGDSSKLRPGQMVVAIGSALGDFQNTVTTGVISGLNRKLQRDNGIPMEDLIQ
                     TDAAINHGNSGGPLLDLQGEVIGINTAVVRASGQGDVAEGLGFSIPVNTVKTITSQLI
                     AKGRVPRPYLGVNTRLVTPAMASYFGLRDESGRLLDHGVVVVSVTPGSPAEAVGLRVG
                     DVVLDINGQQIDETNVLANILTHFAVNDKVTLTVVRNSQTIKVPVTLTERP"
BASE COUNT          423 a         1044 c         1098 g          514 t
ORIGIN      
        1 tcaccgacac gccatactgc gcggcgatgc tggtcatggt ttcgccgtac tggacggtat
       61 gggtgcggcc cgcggccacc gccgcgcccg gcagcagcag caacacgctc agcgcaaccg
      121 ccaggagatg gatagagagc cgtttcatat gagaacttcc tccggagtgg cgtggggccg
      181 cgactgtggg cgtcacaccc gggtagctgg ctcccgcaca gcacggcgcc atatcagcac
      241 ttgcaagtgc tccaatttat catagcaccc cttcttgctt atgtcaatag cctggcgcgg
      301 gacgattgac ataaatgctt gatatgtatg acgaatccgc cctgctcagg ttacgtccac
      361 gccgcgccct tcgaccactt cgccgatgcg gtagaccagc acgcccgcat cgcccagccg
      421 ggcgctcaac tcgccgtatt cgagcggcgg cacggcggcc agcaacccgc cggaggtctg
      481 cgggtcgagc agcagcgcct ggagcgagtc ggggaccggg cggcgcacgt gcacgcgggg
      541 ttgggtcagc cccgcagccg agtcgggttc catgagatag gcgtagttgc gcccgccgcc
      601 gccggtggtc aggcccgcct cggcgtagtc ccaggcgccc gccagcagcg gcacctcgtc
      661 ggcttcgatc accaactgca cgccgcccgc ttcggccatc tcgtaggcat ggcccagcag
      721 gccgaagccg gttatgtcgg tggcggcatg gacgccgcag gcgatcaggg cttcgctggc
      781 cgcccggttc agttcggtca tgctcgccac ggcgccggcc aggtcggcgg ggcgggcgcg
      841 gtcggcgcgc gccgccgtgg tgatcacccc ggtgccgacc ggcttggtga gcacgagcac
      901 gtcgttcggt tgggcgccgc ccttggtcag caggtgcgcc gggtgcacga tcccggtcac
      961 ggacaggccg tacttgggtt cgcggtcggt cacggtatgg ccgccggcca cgatggcccc
     1021 ggcctcggcc accttatcgg ccccgccttg cagcacggca gccagcagcg cccggtccag
     1081 gtcctccggc cacatggcga tgttcagggc caggatcggg tggccgccca tggcgtatac
     1141 gtcgctcagc gcgttcgtcg ccgcgatggc cccgaacagg taggggtcgt cgacaatcgg
     1201 cgggaagaag tcgagcgttt gcaccagcgc ctgatccgcg ttcagttgat agacggcggc
     1261 gtcgtcgctc gtatgcagcc ccaccagcag cgccgggtgg gtatgtggtg tcaggtgacg
     1321 cagcacgtgc gccaggtccc ccggacccat cttgcccgct cagccggcgc aggtcgccag
     1381 gctggtcagg cgcacgatgg cgcggttgtc gtccatgggg atcacctcct tccgttggga
     1441 tgggcgccgg tcacgggcgt tcggtcagcg tgaccggcac cttgatggtt tgcgaattgc
     1501 ggaccacggt cagcgtcacc ttgtcgttga cggcgaaatg ggtcaggata ttcgccagga
     1561 cgttggtttc gtcgatctgc tggccgttga tgtccaggac cacgtcgccc acgcgcaggc
     1621 ccaccgcctc ggcggggctg ccgggcgtca cgctcaccac gaccacgccg tggtcgagca
     1681 gcctgccgct ctcgtcgcgc agcccgaagt acgaggccat ggccggcgtc accaggcgcg
     1741 tgttgacgcc caggtagggc cggggcaccc ggcccttggc gatcaactgg ctggtgatcg
     1801 tcttgaccgt gttgaccggg atggagaagc caaggccctc ggccacatcg ccctggccgc
     1861 tggcgcgcac cacggcggtg ttgatgccga tcacctcgcc ctgcaggtcg agcagcggcc
     1921 cgcccgagtt gccgtggttg atcgcggcgt cggtctggat caggtcttcc atcgggatgc
     1981 cgttgtcgcg ttgcagcttg cggttgaggc cgctgatcac gcccgtggtc acggtgttct
     2041 ggaagtcgcc cagcgccgag ccgatggcga cgaccatctg gccggggcgc agcttgctcg
     2101 aatcgcccag cgtggcgacg ccgggcacgg tgccatccac tttcagcacg gccaggtcgc
     2161 tgatgtcgtc gctgccgacg agggtcgcgt tgacggtgcg cccgtcggag aaaatgacct
     2221 gcaacgcgct gttgttggcg acgacatggt tgttggtgac gatatagcct ttggcgtcga
     2281 tgactacgcc gctgccgctc gcctcgccgc cgaagccctg ctgcggatca agcttgttga
     2341 cgaccgtgac caccgccggg cccaccgcat cgacgacgcg ctcaaacatt tcagccgcgc
     2401 tcggaatccc ggtcagcggg gatgtgccgg tcgtcgccgt ggccggggcg ctcgatccgc
     2461 tgggggtggg cgtgccggtg ccggtggtcg gcgtggtgac gccgctgccg gtcgtgggcg
     2521 tggcgaaggc gctacctgtg gtcggcgtgg ccggcgcgcc ggtggtggcc gtggggatgg
     2581 cgttgggcgt gggggtggcc gcctgcgcgg gcgcggtcgg cgtcacgacg gcagcggcgg
     2641 tcggagttgc cccggcttcc gggcgcaggt tgctcagggt gcagcccggc gcggcgatca
     2701 gggcagccag gaccagggcg ggcacgaacg cccgccggat cgcggcgccg gggcggctgg
     2761 taaattcgct ggtgctgctc atgacgcagg ttcactcctc tttcgcaagg ggcggatcag
     2821 cccattatag catggacggg cgcgccgtgc agggccgccc ggaggctcaa gcctccggct
     2881 acggcgacga aggccgcctg cgcggcctgt ctcctctggc ccagcccgcc ccttcagggc
     2941 cgcccggagg ctcaagcctc cggctacggc gacgaaggcc gcctgcgcgg cctgtctcct
     3001 ctggcccagc ccgcgcaggc gggcttcgta aggctagccc gccccttcag ggccgcccgg
     3061 aggctcaagc ctccggcta
//
LOCUS       DASUYC010000860         2617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_598039,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000860 DASUYC010000000
VERSION     DASUYC010000860.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598039"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2520)
                     /locus_tag="VF276_14130"
     CDS             complement(<1..2520)
                     /locus_tag="VF276_14130"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX6608039.1"
                     /translation="MIDPVSTAPDDDALAAPAGALSALAALAGQRFATLSAALDAVLP
                     LITTHLGLRTAFCARIAGDAAEIVAARTLPGGCAVMPGSVLPLPHLVCSLIAAAPDPE
                     PLAIADTRTDPRTAPLALPLAIPHIRSYIGVPILLHDGTFYGTLCAVDPDPRPLAAPQ
                     VQVLTVLAAFLAPDIERERAEAAAAAQRDFAQQVMETMGQGLTVTGADGRFEYVNPAY
                     ARMVGYPPAALLGKTPGDVTAPEDHALLAQAWAERRAGAATTYETRLIHADGHIVPVL
                     ITGAPRRVGDEIAGTIAVVSNLTGRKQIEAARQESEDRYRQLVELAPVAVLVVSDDRI
                     AYSNPAGLRLLGAAAPDEVLGVPPLDFVHPDYREAAAERMEQARDARGIAPLHEEQLV
                     RRDGVVLDVETTLAPLTYLGRPAVQVVIQDITARKRAEAAWRESETRFRTFVESLGEG
                     LLITDPDDVVIYANARMAELTGYPVDEIIGVTAYTLFLPAAEDWAAMRARTRRRMDGQ
                     AEQYSIHLRRRDGSRFWVEINARPLRDTAGTVVGTVGAVTDITQRRKVEEALRRQNDE
                     LAALHDMTLGIINRLNLSNLLEAVVLRAATLMRTEDCFLALVDPTDEAMVCQVGAGLF
                     TGFQGYHMPKGTGITGRIRAGGGPILIDDYATWPDRSPDFERLPLHAMVAVPLQAGGK
                     LAGAISVAQVREGRRFDPESIALLTRLAQLASLALDNARLYTAARQDIEDRRRAEEAL
                     RHSQELLHAVATGAPVVLFALDLDGCITLVEGRALTVLGLSPGALVGRPIGALFPDLR
                     TLAADIPRILAGEPYAAVLTMDGRTFQAELAAALAAQQTANE"
BASE COUNT          329 a          792 c         1131 g          365 t
ORIGIN      
        1 ttcgttggcc gtctgctggg cggccagggc ggcggcgagt tcggcctgga aggtgcgccc
       61 gtccatggtg aggacggcgg cgtagggctc gccggccagg atgcgcggga tatcggcggc
      121 gagcgtgcgc aggtcgggga agagggcgcc gatgggccgg cccaccaggg cgccggggct
      181 gaggccgagc acggtgaggg cccggccctc gacaagggtg atgcagccgt cgaggtcgag
      241 cgcgaagagc acgacgggcg ccccggtggc gacggcgtgg agcagttcct ggctgtggcg
      301 cagggcctcc tcggcgcggc ggcggtcctc gatgtcctgc cgggcggcgg tgtagaggcg
      361 ggcgttgtcg agcgccagcg aggcgagttg ggccaggcgg gtgagcaggg cgatagactc
      421 ggggtcgaag cggcggccct cgcgcacctg ggccacgctg atggccccgg ccagcttgcc
      481 gccggcctgc aagggcacgg cgaccatggc gtgcaagggc aggcgctcga agtcgggcga
      541 gcggtcgggc caggtggcgt agtcgtcgat gaggatgggg ccgccgccgg cgcggatgcg
      601 cccggtgatg ccggtgcctt tgggcatatg gtagccctgg aagccggtga acagcccggc
      661 gcccacctgg cagaccatcg cctcgtcggt gggatcgacc agggccagga agcaatcctc
      721 ggtgcgcatg agggtggcgg cgcggagcac gacggcttcg agcaggttgc tcaggttgag
      781 gcggttgatg atgcccaggg tcatatcgtg cagggcggcc agttcgtcgt tctggcggcg
      841 caacgcctcc tcgaccttgc ggcgctgggt gatatcggtc acggcgccga cggtgccgac
      901 gacggtgccg gcggtgtcgc gcaggggccg ggcgttgatc tcgacccaga agcggctgcc
      961 gtcgcggcgg cgcaggtgga tgctgtattg ctcggcctgg ccgtccatgc ggcggcgggt
     1021 gcgggcgcgc atggcggccc agtcttcggc ggcgggcagg aagagcgtgt aggcagtcac
     1081 gccgatgatc tcgtccacgg gatagccggt gagttcggcc atgcgggcgt tggcgtagat
     1141 gaccacgtcg tcggggtcgg tgatgagcag gccctcgccc aggctctcga cgaaggtgcg
     1201 gaagcgggtc tcggactcgc gccaggcggc ctcggcgcgt ttgcgggcgg tgatgtcctg
     1261 gatgacgacc tgcaccgccg ggcggcccag gtaggtcagc ggggccaggg tggtctccac
     1321 gtcgagcacg acaccgtcac ggcggacgag ttgctcttcg tgcagcgggg cgatgccgcg
     1381 ggcgtcgcgg gcctgttcca tgcgctcggc ggcggcctca cggtagtcgg ggtggacgaa
     1441 gtcgagcggc gggacgccca gcacctcgtc gggggcggcg gcgccgagca ggcgcaggcc
     1501 cgccgggttg ctgtaggcga tgcggtcgtc gctgacgacc aggacggcga cgggggcgag
     1561 ttcgaccagt tggcggtagc ggtcctcgct ctcctggcgg gcggcctcga tctgcttgcg
     1621 cccggtgagg ttgctgacca cggcgatggt gccggcgatc tcgtcgccga cgcggcgggg
     1681 ggcgccggtg atgagcacgg ggacgatgtg gccgtcggcg tggatgaggc gggtctcgta
     1741 ggtggtggcg gcgccggcgc ggcgctcggc ccaggcctgg gccagcaggg cgtggtcttc
     1801 gggcgcggtc acgtcgccgg gggtcttgcc gagcagggcg gcgggcgggt agccgaccat
     1861 gcgcgcgtag gcggggttga cgtattcaaa gcggccgtcg gcgccagtga cggtcaggcc
     1921 ctggcccatg gtctccatga cctgctgggc gaaatcgcgc tgggcggcgg ccgcggcttc
     1981 ggcgcgctcg cgctcgatgt cgggggccag gaaggcggcc agcacggtga gcacctggac
     2041 ctggggggcg gccagcgggc gcggatcggg atcgacggcg cagagggtgc cgtagaaggt
     2101 gccgtcgtgc agcagaatgg gcacgccgat gtagctgcgg atgtgcggga tggcgagggg
     2161 cagggccagg ggggcggtgc gcgggtcggt gcgggtgtcg gcgatggcga gcggttcggg
     2221 gtcgggcgcg gcggcgatga ggctgcagac caggtggggc agcggcagca cgctgcccgg
     2281 catgacggca cagccgccgg gcagcgtgcg ggcggccacg atttcggcgg cgtcgccggc
     2341 gatgcgcgcg cagaaggcgg tgcgcaggcc caggtgggtg gtgatgagcg gcaacaccgc
     2401 gtcgagcgcc gcgctgaggg tcgcgaaccg ttggcccgcc agcgcggcca gggcgctgag
     2461 cgcccccgcc ggcgcggcca gggcatcgtc gtccggcgcg gtgctgacgg gatcgatcat
     2521 gtgtgtcatc caatcctcgc ggggctgcgg cccggtggcg gcaccggggc cacacccggt
     2581 cgcttcgggc aaagtatacc gcgcccggcg cgcgaac
//
LOCUS       DASUYC010000861         3593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1753873, whole genome shotgun sequence.
ACCESSION   DASUYC010000861 DASUYC010000000
VERSION     DASUYC010000861.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1753873"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2251
                     /locus_tag="VF276_14135"
     CDS             <1..2251
                     /locus_tag="VF276_14135"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEX6608040.1"
                     /translation="LVVALLGAGAPSIAGPAAGPPPPLPAPPVQPTPVPPAPFSVLSP
                     NGNPNLDYGFTMALREDLPRLESDGFQWAEFEISWADAQPSPTGPYDWGHVNNIIQAM
                     GGRDGSHLGIIFRVDQSPAWANSAGVAGWYPPRDPNTYARFIGDMAAYVSGKLRYAPA
                     YEIWNEPNISENWGGACPDPEFYTAMVQAAYPAIKRGDPYSPVLAGAVTTVGALPSSP
                     GSCHVDDLEYLNRMYDAGVKGYFDALSTHPYGFGDVPEANPRTPGRTLVFRRAELQRE
                     VMVAHGDAARHMWITETGWAIDPASINGNSCRSSCPNCYDWYFQWNEQQQADNLVRAF
                     NWARSYWAWMDTIVVFAFDYNQASWIGPCGSFAYFSVMGRPAENQLSANRLFPPPTYT
                     PLPTATPTPAVDDPPVVGQTRLDPPSYWRTGGPLTVALDASDTDNSRVASGFFQVTYP
                     GGQIQTVGMALVGGTNASGTWAGTFAVPNPSGNADEVYGIRACVVEDFPPRRVVCADP
                     VNFTVSTSRFSDVPRDFWAYPYIEYLAAQGIISGYADNTFRPNNNITRGQVAKMITLG
                     QGWPLLNPPTPTFIDVPQAHPFYQYIETVAAHGVVSGYDDHTFRPGNNITRGQISKMI
                     ALGEGWALANPPTPTFSDVPPAHPFYTYIETVAAHGVVSGYADGTFRPNANATRAQLS
                     KMLYIAISSRATPTPTVTATATATATSTPAPSATVTATPATPTATPTGTAIASPTPTG
                     TATPGKPAP"
     gene            2440..3489
                     /locus_tag="VF276_14140"
     CDS             2440..3489
                     /locus_tag="VF276_14140"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6608041.1"
                     /translation="MALPVPAPAAAPESATPACPHPDAQQHPLFAARDYISGDLFLIQ
                     RCAACGLVRTAPQPSAETIADYYPRGYYGQGKRYSMPLERLLDWLYAFRGRLIEIANG
                     LEPGRVLDIGCGRGLLLHQLRKRGWEVTGTELTEASASYARDVLRIDVKTGSLAELHL
                     PDESYDTVILWHVLEHIPDPAALLAEVARILRPGGTLLVAVPNFGSLEARWARDHWFH
                     LDVPRHLNHFTTASLRRMLAGAGLHPRMTSYFASEYDFFSFVQSALNMLGIRHNLLYN
                     LLRTRGAKVLAGRRQQAARPRDLALTLLLTPGLGLISLAWAPLAALLGQGATVTIYAK
                     KPRQTSGQWSVVSDQ"
BASE COUNT          572 a         1372 c         1130 g          519 t
ORIGIN      
        1 gctggtggtg gcgctgctgg gtgcgggcgc gccgagcatc gccggccccg ccgcgggccc
       61 gcccccgccc ttgcccgcgc cgcccgtcca acccacgccc gtgccgcccg cgcccttcag
      121 cgtgctcagc cccaacggca acccgaatct cgactacggc ttcaccatgg cgctgcgcga
      181 agacctgccc cgcctggaga gcgacggctt ccagtgggcc gagttcgaga tcagttgggc
      241 cgacgctcag cccagcccca ccggcccgta tgactggggc cacgtgaaca acatcatcca
      301 ggcgatgggg ggccgcgacg gcagccatct cggcatcatc ttccgcgtgg accagtcgcc
      361 cgcctgggcc aacagcgcgg gcgtggccgg gtggtacccg ccgcgcgacc cgaacaccta
      421 cgcccgcttc atcggcgaca tggccgcgta tgtgtcgggc aagctgcgct acgcgcccgc
      481 ctatgagatc tggaacgagc ccaacatctc ggagaactgg ggcggcgcct gcccggaccc
      541 ggagttctac acggccatgg tccaggccgc ctacccggcc atcaagcggg gcgaccccta
      601 ttccccggtg ctggccgggg ccgtgaccac cgtgggcgcc ctgcccagca gccccggctc
      661 ctgccacgtg gacgacctgg agtacctcaa ccgcatgtat gacgcgggcg tcaagggcta
      721 cttcgacgcc ctgagcacgc acccctatgg cttcggcgac gtgcccgagg ccaacccgcg
      781 cacgccgggg cgcacgctcg tcttccgccg cgccgaactc cagcgcgagg tgatggtggc
      841 ccacggcgac gccgcgcgcc atatgtggat caccgagacc ggctgggcca tcgacccggc
      901 ttcgatcaac ggcaactcct gccgctccag ttgccccaac tgctacgact ggtacttcca
      961 gtggaacgag cagcaacagg cggataacct ggtgcgcgcc ttcaactggg cgcggtccta
     1021 ctgggcctgg atggatacga tcgtcgtctt cgccttcgac tataaccagg cgtcgtggat
     1081 cgggccgtgc ggctcgttcg cctacttcag cgtgatgggg cgccccgccg agaaccagct
     1141 cagcgccaac cggctcttcc cgccgcccac ctacacgccg ctgcccacgg ccacgccgac
     1201 gcccgccgtg gacgacccgc cggtggtggg gcagacgcgg ctcgacccgc cgtcatactg
     1261 gcgcaccggc ggcccgttga ccgtggccct cgacgccagc gatacggata actcgcgggt
     1321 tgcatccggc ttcttccagg tgacgtaccc cggcggccag atccagacgg tcggcatggc
     1381 cctggtgggc ggcacgaatg ccagcggcac ctgggccggc acgttcgccg tgcccaaccc
     1441 gagcggcaac gccgacgagg tctacggcat ccgcgcctgc gtggtcgagg acttcccgcc
     1501 gcgccgggtg gtctgcgccg acccggtgaa cttcaccgtc agcacatcgc gcttcagcga
     1561 cgtgccgcgc gacttctggg cctatcccta catcgagtac ctggcggcgc agggcatcat
     1621 ctcgggctat gccgacaaca cattccgccc gaacaacaac atcacgcgcg ggcaggtcgc
     1681 caagatgatc accctggggc agggctggcc gctgctcaac ccgcccacgc cgaccttcat
     1741 cgacgtgccg caggcgcacc cgttctacca gtacatcgag acggtggccg cgcacggcgt
     1801 ggtctccggc tacgacgacc acacgttccg gcccggcaac aacatcaccc gcgggcagat
     1861 ctcgaagatg atcgcgctgg gcgaaggctg ggcactcgcc aacccgccca cgcccacctt
     1921 cagcgatgtg cccccggcgc acccgttcta cacctacatc gagaccgtgg cggcccacgg
     1981 cgtggtgtcg ggctacgccg acggcacctt ccggcccaac gccaacgcga cccgtgccca
     2041 gttgagcaag atgctctaca tcgcgatcag cagccgcgcg acgcccacgc ccacggtcac
     2101 ggctaccgcc acggccacgg cgaccagcac cccggcgccc agcgcgaccg tcacggccac
     2161 gccggccaca cccacggcca cgcccaccgg cacggcgatc gcctcgccga cgcccacggg
     2221 cacggccacg ccgggcaaac ccgcgccgta agcccgcgcc gatttcccac cgcgaaggcg
     2281 cgaaggacgc gaagaataac gtttataata acgttcttcg cgttcttcgt tccttcgcgg
     2341 tgcacgtctg cacggctatc cggcagcggc ggttgacatt gccgaggcgc ggatgttaga
     2401 atcagccgag ccatcaccac cgactagtag gagatttcct tggccctccc tgtacccgcg
     2461 ccggcggctg cgccggaatc cgcaaccccc gcctgcccgc atcccgacgc ccagcaacac
     2521 ccgctctttg cggcgcgcga ttatatcagc ggcgacctgt ttctgatcca gcgctgcgcc
     2581 gcctgcgggc tggtgcgcac cgcgccgcag cccagcgccg agaccatcgc ggactactat
     2641 ccgcgcgggt actacggcca gggcaagcgc tacagcatgc cgctggagcg gctgctggac
     2701 tggctctacg ccttccgcgg gcgcctgatc gagatcgcca atgggctgga gccgggccgc
     2761 gtgctcgaca tcggctgcgg gcgcggcctc ttgctgcacc agttgcgcaa gcgcggctgg
     2821 gaagtgaccg gcaccgaact caccgaggcc agcgcgagct atgcccgcga cgtgctgcgc
     2881 atcgacgtga agaccggcag cctggccgag ctccacctgc ccgacgagag ctacgacacg
     2941 gtgatcctct ggcatgtgct ggagcatatc cccgacccgg cggcgctgct ggctgaggtg
     3001 gcgcgcatcc tgcggcccgg cggcacgctg ctggtggccg tgccgaactt cgggtcgctc
     3061 gaagcccgct gggcgcgcga ccactggttc cacctcgacg tgccgcgcca cctgaaccac
     3121 ttcaccacgg ccagcctgcg gcggatgctg gcgggggccg ggctgcaccc gcgcatgacc
     3181 agctatttcg cctcggagta cgacttcttc agcttcgtgc agagcgcgct caacatgctg
     3241 ggcatccgcc ataacctgct gtacaacctg ctgcgcacgc gcggggccaa ggtgctggcc
     3301 gggcggcggc agcaggcggc ccgcccgcgc gacctggcgc tcaccctcct gctcaccccc
     3361 ggcctgggcc tgatcagcct ggcctgggcg cccctcgccg ccctgctggg ccagggcgcc
     3421 acggtcacga tctacgccaa gaagccccgg cagacgagtg gtcagtggtc agtggtcagt
     3481 gatcagtaat gcggtaggca cgagactctt cgctgcgctc agagtgacag gcttcccgcg
     3541 cgcgacgaga ctcttcactg cgctcagagt gacaagccac gcgctgacca ctg
//
LOCUS       DASUYC010000862         2559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1291923, whole genome shotgun sequence.
ACCESSION   DASUYC010000862 DASUYC010000000
VERSION     DASUYC010000862.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291923"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VF276_14145"
     CDS             <1..308
                     /locus_tag="VF276_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018755987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX6608042.1"
                     /translation="QPIMPDYIGKLRAEFAAEPPQSAATADRPPAAAARPLGEPLSQR
                     ELEVLHLIAQGLSNQEISARLFLALSTVKGHNREIFAKLQVQRRTEAVARGRELGLL"
     gene            535..1350
                     /locus_tag="VF276_14150"
     CDS             535..1350
                     /locus_tag="VF276_14150"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HEX6608043.1"
                     /translation="MITLTREETNKTTPGNGSTVEASSRSTRMMGSRRAYSGLIGGLF
                     LAAFLCYGLGNALATSVLGGPNYLVTISAQQPTLALGVLLMLVNSMAVAGIGVVFFPI
                     LEQHGPRTALAYLAARIVEAVLLAIGALVLLLIVPLGQVGAAGAASGGWVTTIGSLAV
                     QANTNFYQAGEMSLALGGVFLCPLLYRTGLIPRVLAAWGAIGYAIFLTGTVAEILGLP
                     IGLVLSIPGGLWELVFGFWLISKGFQPAAYAAGAAVTGAGVTTPAVRPAPAAQ"
     gene            1479..1751
                     /locus_tag="VF276_14155"
     CDS             1479..1751
                     /locus_tag="VF276_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608044.1"
                     /translation="MATNRTSTLDPGQPPIYQIRLQGHLSRQWTDWFGGLTITLEDNG
                     DTLLTGVVVDQAALHGVLRKVRDLGIPLLAINRVETGQPGASGVQQ"
     gene            1999..>2559
                     /locus_tag="VF276_14160"
     CDS             1999..>2559
                     /locus_tag="VF276_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013334435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6608045.1"
                     /translation="MSLALALIAGLALAGASYEAIAARGDAQTFPPPGRLVDVGGYSL
                     HIQCVGSGSPTVVFDAGLGGTSLDWTLVQAVIGQTTRVCTYDRAGMGRSDNGLAPRTP
                     AQIAGELHTLLGNAGLAGPYVLVGHSLGGITISAVAEARRRKLKALVYLCALLPTSGK
                     TGKDMTSLEPETMFRLSRQVSPDGLTY"
BASE COUNT          450 a          831 c          828 g          450 t
ORIGIN      
        1 cgcagccgat catgccggac tatatcggga agttgcgggc ggaatttgcg gcggagccgc
       61 cgcagagcgc cgccactgcg gaccggcctc cggccgccgc ggcccggccc ctgggcgagc
      121 ccttgagcca gcgggagtta gaagtgctgc acctcattgc ccagggcctc tcaaatcagg
      181 agattagcgc ccggcttttc cttgccttga gtaccgtgaa agggcacaat cgggaaatct
      241 tcgccaaact ccaggtccaa cggcgcaccg aagcggtcgc gcggggccgc gagttgggtc
      301 tgctgtaggc gcaccgtccg tggcccccgc cctgggggcg ggtcgtgccg acccgcaagc
      361 gaggtatgtg gtcaaagctt tagccctgct acccgctccg gcaccccaca acaatacccc
      421 ccagcaatac tttagtagct acacggcaat accgcatcat cgctatgctc gtggtacatc
      481 gatcgcgtgg catcgatgtc gccaatctcg ccatagtcaa agaggagcat aacgatgata
      541 acgctgacca gggaagaaac caacaagacg accccaggca atggatcaac cgtagaagcg
      601 agttcaagga gcacccggat gatgggttcc cgccgagcgt attcggggct gatcggcggg
      661 ctgtttctgg cggcattcct ctgctacggt ctgggcaacg cattagcgac ctccgtcctc
      721 ggcggcccca actacctggt gacgatttcc gctcagcagc cgaccttggc cctcggcgtc
      781 ttgctgatgt tggtgaattc catggcggtg gccggtatag gcgtggtgtt cttcccgatc
      841 ttggagcaac atggcccgcg aaccgcgctg gcctacctgg ccgcgcggat cgttgaggcg
      901 gtcctgctgg ccatcggggc gctcgtgcta ctcctgatcg ttcccttggg ccaagtgggc
      961 gcggccgggg cggcgagcgg gggttgggtc accaccatcg ggtcgctcgc ggtccaggcg
     1021 aacaccaact tctatcaggc cggcgagatg tcgctcgccc tcgggggtgt gtttctgtgt
     1081 cccttgcttt accggacggg gctgattccc cgcgtcctcg cggcgtgggg tgcgatcggc
     1141 tatgcgattt tcctcacggg caccgtcgcc gaaatcctcg gcctgcccat cggcctcgtg
     1201 ctctcgatcc ccggtggtct ctgggagttg gtattcgggt tctggctcat cagcaagggt
     1261 ttccagccgg cggcctatgc cgccggtgcg gcggtcacgg gggcgggggt gacaaccccc
     1321 gccgtgcgcc cggcgccagc cgcgcagtag gcgaaggcga gacgcgaggt atcaccagca
     1381 caccacgcgg ttcgccacaa caggtacagg acggtcctgt acctgttgtg ggatcggatg
     1441 tctgcacggt tgttggatag aagacggggc agatcagcat ggcaaccaac cgcacctcaa
     1501 ccctagaccc gggccagccg ccaatctatc aaatcagact ccagggtcac ctgagtcggc
     1561 aatggacaga ttggtttggc ggcctgacca tcaccctgga ggacaacggc gatacgctgt
     1621 taacgggcgt ggtggtcgat caggccgcgt tgcatggcgt gctaagaaag gtgcgcgact
     1681 taggtatccc cttgcttgcg atcaaccgtg tggagaccgg ccagccaggc gcatctggtg
     1741 ttcaacagta aatagtggca agcgcccgga gaaaggaagt tggctgctgc ccgcacatac
     1801 tcgcctgccg cagggctagg gacggcgttc gtccgatggc gcaagctcag cagatagctt
     1861 catcatccac cactgtgaaa tgaccaaagc aaggcaagct tggagaaacc cacaacggca
     1921 actgtaacga acacgctgtc tgtaccgacc tccgcccggc gcatgggccg gcgcgtctta
     1981 cgcgggatcg cccgttggtt gagcctagcg ctcgccctga tcgcgggatt ggcgctggcc
     2041 ggggcgagct atgaagccat cgccgcgcgg ggcgatgcgc aaacctttcc gccgccgggc
     2101 cgcctggtcg acgtcggcgg ctacagcctg catatccagt gtgtcgggag cggcagcccg
     2161 accgtcgtgt tcgacgccgg gctgggcggc acgtcgctcg attggaccct ggtgcaagcg
     2221 gtgatcggcc agacgacgcg ggtctgtacc tatgaccggg ccggcatggg caggagcgac
     2281 aacggcctgg cgccgcgcac ccccgcccag attgccggcg aactgcacac cctgctgggc
     2341 aacgccggcc tcgccggccc ctacgtcctg gtcggccact cgctgggcgg catcacgatc
     2401 agcgccgtcg ccgaagcacg gcggcgcaag ctcaaggcgc tggtctacct ctgcgcgctg
     2461 ctgcccacct cgggcaagac cggcaaggac atgacctcgc tcgaacccga gacgatgttc
     2521 cggctatcgc ggcaggtcag ccccgacggc ctcacctac
//
LOCUS       DASUYC010000863         2230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_613447,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000863 DASUYC010000000
VERSION     DASUYC010000863.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613447"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VF276_14165"
     CDS             complement(<1..613)
                     /locus_tag="VF276_14165"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6608046.1"
                     /translation="MAITGQAPAAPGYADDPRQPGALPAFVPLRIPPLPYEDIRELLD
                     DPQEAGERELRQSLRDIRMANIVGQGTAVVLRHLPDVLGDWPAGRPVRVLDLATGSGD
                     IPRAVVRWCRRRGIPCRVLATDISPHVLRETRHHCRAFPEIEVLVCDSRAVPFPPGSF
                     DLVTCSLALHHLDTEGATRTLAAMARLARRGFIVNDVERSWPAY"
     gene            complement(698..1225)
                     /locus_tag="VF276_14170"
     CDS             complement(698..1225)
                     /locus_tag="VF276_14170"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX6608047.1"
                     /translation="METIYKGIAQGTEIVDKNGHKIGTVHETIINSADELQMLTVQRG
                     LIFKHDVGIPVASIDRVEGGKVYLSVGKDTLTELMHPDVAKDPLAGRDTTAADVAPNP
                     DLLEREVTDRRPNQAAFTPDLPHDRATAGYTFEEGLGTAGTPGVGRGDAVLTSEGEAG
                     INPASSQDPTGPDRT"
     gene            1629..>2230
                     /locus_tag="VF276_14175"
     CDS             1629..>2230
                     /locus_tag="VF276_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608048.1"
                     /translation="MRRSSFIGAMLIGMLLLLGSLQAVSPPARANLPNAVAPNGAAPV
                     APAPPAAVAVANRDPAPPKDAPDRSLPAGAPAGPTAPDLITAGTYNFTSGTGVALQDM
                     SSGTTQLVGAGLDDTASALTNIGFDFWYDGVRVTQFSANANGLMRLGSTVIGTSFTNS
                     LASTTEAPKIAPFWDDLCTGTNGKIHYKVAGSAPNRTLVV"
BASE COUNT          356 a          790 c          721 g          363 t
ORIGIN      
        1 ggtacgcggg ccacgaccgc tcgacatcgt tgacgatgaa gccccgccgg gcgaggcgcg
       61 ccatggcggc gagcgtgcgg gtcgccccct cggtatcgag atgatgcagg gccagcgaac
      121 aggtgaccag atcgaacgag ccgggcggga acggcacggc ccgcgagtcg cagaccagca
      181 cctcgatttc ggggaaggcg cggcagtggt gccgggtttc gcgcaagacg tgggggctga
      241 tgtcggtggc cagcacccgg caggggatgc cccgccgccg gcaccaccgc accacggccc
      301 gcgggatgtc gccggagccg gtcgccagat cgagcacgcg caccgggcgc ccggcgggcc
      361 agtcgccgag cacgtcgggc aggtggcgca gcaccaccgc cgtgccctgg ccgacgatgt
      421 tcgccatgcg gatatcgcgc aggctctggc gcaactcgcg ctcgccggcc tcctgcgggt
      481 cgtcgagcag ttcgcggata tcctcgtagg gaagaggggg aatgcgcagg ggcacaaaag
      541 ccgggagggc gccgggctgc cgtggatcgt cggcatagcc cggcgccgcg ggcgcctggc
      601 cggttatcgc catacagtcg tctccgagat ctgtggaacg cgtttaccgc gcggatcgca
      661 tcacaccatc atacgcggcg gaccgggcgg gcggatgtca ggtgcgatcc gggccggtcg
      721 ggtcctgcga cgacgcgggg ttgatgcccg cctcgccctc ggaggtcaag acggcgtcgc
      781 cacgccccac accgggggtg cccgccgtgc ccagtccctc ctcgaaggtg tagcccgcgg
      841 tggcgcggtc gtggggcagg tcgggcgtga aggcggcctg gttgggccgg cgatcggtca
      901 cttcccgctc cagcaggtcg ggattgggcg ccacatccgc cgcggtggta tcgcgcccgg
      961 ccagcgggtc cttggccaca tcggggtgca tcagttcggt gagcgtgtcc ttgccgacgc
     1021 ttagatagac cttgccgccc tcgacccggt cgatggaggc caccggaatg cccacgtcat
     1081 gcttgaagat cagccctcgt tggacggtta gcatctggag ttcgtccgca ctgttaatga
     1141 tcgtttcgtg caccgtgccg atcttgtgcc cgtttttatc gacgatctcg gtgccctgtg
     1201 caatgccttt atagattgtt tccatggtcc cacctcctgg ccctggcggt gcaagtagcg
     1261 tgccatccag gcccagccca aaccatgtgt cgtttagcgc ccaatgtccg ggtttccgcg
     1321 caaatgtcca ccgcgcgcaa gtgccctgcc gcggccatcg cttaccggat cgtaaagttg
     1381 aatatgccgc agaattctgc tagaataagg taggtctgga cgcgccctgt gtccgacccg
     1441 tctgcctcca tccggcacgg ttgttcccga ttcatggttc catatttctc acgcctcggg
     1501 cgcaccggcc cgagacaata ccggtgcgcg tctccggcgc ggcccataag ataccccgcg
     1561 tccgtcacaa ctcgttagcg cggccccaac atcaacccgc gctaaccttg cttcaggaag
     1621 ggagcctcat gcgacgttct agtttcatcg gcgccatgct catcggcatg ctcttactgc
     1681 tcggcagcct ccaggcggtg agtccgccgg cgcgggccaa tctgcccaat gcggtcgcgc
     1741 ccaacggcgc cgcacccgtc gcccccgcac cgcctgccgc ggtcgcggta gccaaccgcg
     1801 acccggcgcc gccgaaggac gcgcccgacc gcagcctgcc cgccggcgcg cccgccggcc
     1861 ccacggcacc cgatctcatc accgcgggca cctacaactt cacctccggc acgggcgtgg
     1921 ccctgcaaga catgagcagc ggcaccacgc aactcgtcgg cgccggtctg gacgacaccg
     1981 catccgccct gaccaacatc ggcttcgact tctggtatga cggcgtgcgc gtcacccagt
     2041 tttcggccaa cgccaatggt ctgatgcgcc tcggcagcac cgtgatcggc accagcttca
     2101 cgaacagcct cgccagcacg accgaagcgc cgaagattgc gcccttctgg gacgacctct
     2161 gcaccggcac caacggcaag atccattaca aagtcgccgg cagcgcgccc aaccgcactc
     2221 tcgtggtcga
//
LOCUS       DASUYC010000864         2351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_370462,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000864 DASUYC010000000
VERSION     DASUYC010000864.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370462"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="VF276_14180"
     CDS             complement(<1..861)
                     /locus_tag="VF276_14180"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6608049.1"
                     /translation="MDILCCPACGQQAWTLEVAQSETLAYHDGPRDEVRTGEVACACG
                     RQYPVREYVLSLAGLFPEDLQREAAFWDRFYLWNLEHGATGFHDLRRGFAPFMAQGVT
                     EAFPHADTIDRYDVHYQVAEHPLMRAGQTLLDIGVGLGWTSIHFARSGYRVTAFDPSL
                     GPVQAAKAYAIREGLQIEYLCAAMGYIQFQPGSFDNVTAFHSLHHVPDLEAGLRDVRR
                     WLRAGGALAVDEHIANSRLAGAVGAQLHQWAAENVFPRYRTIGDADLARLPSEGHSAL
                     EDAGVDQVVPL"
     gene            complement(947..1444)
                     /locus_tag="VF276_14185"
     CDS             complement(947..1444)
                     /locus_tag="VF276_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016766100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEX6608050.1"
                     /translation="MARQGRGALEQTRFRDTGQHLGQYADRFLVRCPRCARCAVVAPL
                     PRESRREPARARVVCDACGYAKDAAQPTKGGAGAVDWYFHLPLWLQAPCCGEVLWALN
                     EAHLAFLEDYVRATLREDIRPDQVSAIAIRNQTMASRLPAWIQSARNRDEILRCIDKL
                     KKTIG"
     gene            complement(1862..>2351)
                     /locus_tag="VF276_14190"
     CDS             complement(1862..>2351)
                     /locus_tag="VF276_14190"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX6608051.1"
                     /translation="SFDELLLLQAGGRYDVFFAGGMQIDAHGNANLLGTAQVRGPGAA
                     GGPLAAQVGRTILYTRNHDRRTFVPQVDHVTLPGWDPGAQRGAPTLVVTPLAVLDFVD
                     GQMRLVSTHGDADAAQVQAATGFPLLIPAGGAPPTPQPSEAVLKQLRAIDEGGLLLLP
                     ES"
BASE COUNT          360 a          799 c          833 g          359 t
ORIGIN      
        1 cagcggcacc acctgatcga cccccgcgtc ttccagcgcc gagtggcctt cgctgggcag
       61 gcgagccagg tcggcatcgc cgatggtgcg gtagcggggg aagacgttct ccgccgccca
      121 ctggtgcagt tgcgcgccca cggccccggc gaggcggctg ttggcgatat gctcgtccac
      181 ggccagcgcg ccgccggcac gtagccagcg ccgcacgtcg cgcagcccgg cttcgagatc
      241 gggcacatgg tgcagcgagt ggaaagccgt cacgttgtcg aagctgcccg gctggaactg
      301 gatatagccc atggcggcgc agaggtattc gatctgcaac ccttcccgga tggcataggc
      361 cttggccgcc tgcaccggcc ccaggctcgg atcgaacgcc gtgacccggt agccggaccg
      421 ggcgaagtgg atcgaggtcc agcccagccc cacgccgatg tcgagcaacg tctgcccggc
      481 gcgcatcagc gggtgttcgg ccacctggta atggacatca tagcggtcga tggtgtcggc
      541 gtgcgggaaa gcctcggtca cgccctgggc catgaatggg gcaaagccgc gccgcaggtc
      601 atggaagccg gtcgcgccgt gctccaggtt ccagagatag aagcgatccc agaaggcggc
      661 ttcgcgttgt aggtcttcgg gaaagagccc ggccagcgac agcacatact cgcgcaccgg
      721 gtattgccgc ccgcacgcgc acgctacctc gccggtgcgc acctcgtcgc gcggcccgtc
      781 gtgataggcg agcgtctcgc tctgcgcgac ctccaacgtc cacgcctgct gcccgcaggc
      841 ggggcagcag agaatgtcca ataatgcacg atccacgtct acaccaggca cggccagccc
      901 ccgtgcctgc ctccttcctc accatagcgc ggcccgccag gccacgctac ccaatcgtct
      961 tcttcagctt gtcgatgcag cgcaagatct cgtcgcggtt gcgggcgctc tggatccacg
     1021 ccggcaggcg gctcgccatg gtctggttgc ggatggcgat cgccgatacc tggtcgggcc
     1081 ggatgtcttc gcgcaaggtc gcgcgcacat agtcttcgag aaaggccagg tgcgcctcgt
     1141 tcagcgccca tagcacctcg ccgcagcagg gcgcttgcag ccagagcggc aggtggaaat
     1201 accagtccac cgcgcccgcg ccgccctttg tgggctgagc cgcatcctta gcgtaaccgc
     1261 aggcgtcgca gaccacgcgc gcccgcgcgg gctcgcgccg gctctcgcgc ggcagcggcg
     1321 cgaccaccgc gcaccgggcg cagcgcgggc agcgcaccag gaaccgatcc gcatattgcc
     1381 ccagatgctg tccggtatcc ctaaagcgcg tctgctccag tgcgcccctc ccttgccggg
     1441 ccattgtacc tgcccctccg cccgcctgtc aaacgagtgc tgagtgctga gtgctgagtg
     1501 ctgagtagtg acgagtggtt tagtgtcttg taggggccgg tttcaaaccg gccggttggg
     1561 tggagggata gggcacggcg gggcacgggc ggctggggcg gcgccggggc gctggaatcc
     1621 acgcttatgg gggcgtgggg cggggcgccc ctatggggtt gcgcgttgcc ggcgggcgat
     1681 cgcgatgcgc ccgtgacaac gaaaaaggct ggaggtccgg accccctcga agcagacagc
     1741 gttggctccg ctgcttctgg taatacaacg ggcgtggcgc cgggtggttg cagccggatg
     1801 gcacgcgtag ccactcggat tatgcccgtt ggggcacagt actgcgcggg ccggaggcgg
     1861 gtcaggactc cggcaagagc aacaggccgc cctcgtcgat ggcgcgcaat tgcttcagga
     1921 ccgcttcgct cggttgcggg gtggggggcg cgccgcctgc cgggatgagg agcgggaagc
     1981 cggtggcggc ctgcacctgg gcggcatcgg cgtcgccgtg ggtcgagacg aggcgcatct
     2041 ggccgtccac gaagtcaagc acggccagcg gcgtcacgac cagcgtgggc gcgccccgtt
     2101 gggcgccggg gtcccagccg ggcagcgtca catggtccac ctggggcacg aaggtgcgcc
     2161 ggtcgtggtt gcgggtgtag aggatggtcc ggcccacctg ggcggccagc ggcccgcccg
     2221 ccgcgcccgg cccgcgcacc tgcgccgtgc ccagcaggtt ggcgttgccg tgggcgtcga
     2281 tctgcatgcc gcccgcgaaa aagacatcgt agcgcccgcc cgcctgcaac agcagtaact
     2341 cgtcgaacga c
//
LOCUS       DASUYC010000865         3309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1756164, whole genome shotgun sequence.
ACCESSION   DASUYC010000865 DASUYC010000000
VERSION     DASUYC010000865.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1756164"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VF276_14195"
     CDS             <1..351
                     /locus_tag="VF276_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608052.1"
                     /translation="YLDSVRRRLPAGESRRYKVAWVGSCVMYDRARLLEVGGFSFWPR
                     LAPDAGAPEMLVQNLLLRRWGGCAILPSGTYYARGMAPARVPTAPQRADGRELLPELI
                     GEYAPPPAIPSWAW"
     gene            370..2199
                     /locus_tag="VF276_14200"
     CDS             370..2199
                     /locus_tag="VF276_14200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX6608053.1"
                     /translation="MTARVSVLMPAYNHAHFIRRALDSLLAQTLAGWELILVDDGSTD
                     ATRSVIAPYLDDPRVRYTRLDRNRGLGVALNQALAQATAPLVAYLPADDVYYAEHLAS
                     LVAALDGHPAAVAAYSGVRHHYNRSAEGAIPGYPLQLVQVLHRRTPDRWVEREEVVTD
                     DLDHMYWTALRAHGDFVPTGRLTCEWVDHPAQLHKVIQEPVGGINLYRQRYQVPHPLR
                     FHSTAGNRIDEVGRYQRYRDRPDTPRATDGLKILLVGELAYNADRVLALEERGHKLYG
                     LWMPDPYWYNTVGPLPFGHVEDLPRDDWRAAIRRVQPDVIYALLNWQAVPFAHEVLRA
                     GLGVPFVWHFKEGPFICLEKGTWNELIDLYTRADGRIYSSPEMGDWFETVVPGIGRLG
                     PALVLDGDLPKRDWFTAPPSSRLSDADGQLHTVVPGRPIGLHPHTVGELAAEGVHLHF
                     YGDFTHGQWKAWIEKARGIAPAHLHLHANVDQEDWVTEFSQYDAGWLHFFQSENAGDI
                     RRANWDDLNYPARIATLAVAGLPLLQGDNAGAIVATQTLARERDLGLFFRDMAELGAQ
                     LRDRARLAQIRANVWRQRDEFTFDHHADRLIAFFREVIAQGAR"
     gene            complement(2277..>3309)
                     /locus_tag="VF276_14205"
     CDS             complement(2277..>3309)
                     /locus_tag="VF276_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608054.1"
                     /translation="LVLLRPARFLAVAVGAGLALRLIGIAVIPLTIRLGDMLPLLQMS
                     TARILQAQNPYIVYQMPWDLPLTYWPLTVLSYLPAALAGIDLRWVNLLFGPLVGALLL
                     LAGGRRGPGPAAHAFGLLYLTPGLFQWDISTAAPPYWLWLCVAFATAVVAARARPGWL
                     LPGAGLGAALAAAPLALPFAPFLGASWLAQGWRVALRRVVVAGVVFAVLVGPFLLWGR
                     RGFLDGAIGWFNDINRLPLLKWQTDKSWALEVGLAGPFWYNSLQDWLKPLQIVLVGGL
                     GVILLALRLRPVRTPAAALRWGAAAYLLFMVVNPVIWPYLYVPALLALAFALVPAGDA
                     APPADAGPV"
BASE COUNT          530 a         1205 c         1137 g          437 t
ORIGIN      
        1 tatctcgaca gcgtgcgccg ccgcctgccc gcgggcgagt cgcggcgcta caaggtggcc
       61 tgggtcggct cgtgcgtgat gtatgaccgg gcgcggctgc tggaggtcgg cggcttcagc
      121 ttctggccgc ggctggcccc cgacgcgggc gcgcccgaaa tgctggtcca gaatctcctg
      181 ctgcggcgct ggggcggctg cgccatcctg ccgtcgggca cgtattatgc tcggggcatg
      241 gcgcccgcgc gggtgcccac cgccccgcag cgtgctgatg gccgcgaact gctgcccgaa
      301 ctgatcggcg aatacgcgcc gccgcccgcc atcccgtcct gggcctggta gcgcgcgaca
      361 ggagaatcta tgaccgcacg tgtgtcggtg ctcatgccgg cttacaacca cgcgcatttt
      421 atccgccgcg cgctcgacag cctgctggcc cagacgctgg ccggctggga actgatcctg
      481 gtggacgacg gctcgaccga cgccacacgc agcgtgatcg ccccctacct ggacgatccc
      541 cgcgtgcgct acacgcggct cgaccgcaac cgcggcctgg gcgtggcgct gaaccaggcg
      601 ctggcccagg ccacggcgcc gctggtcgcc tacctgcccg ccgacgacgt gtactatgcc
      661 gagcacctgg cgagcctggt cgccgcgctc gacgggcacc cggcggcggt ggcggcctat
      721 tccggcgtgc gccaccacta caaccgctcc gctgagggcg ccatccccgg ctacccgctg
      781 caactggtcc aggtgctgca ccgccgcaca cccgatcgct gggtggagcg cgaagaggtt
      841 gtgaccgacg acctggacca catgtactgg acggcgctgc gggcgcacgg cgactttgtg
      901 cccaccgggc ggctgacctg cgagtgggtg gaccacccgg cccaactcca taaggtgatc
      961 caggagccgg tcggcggcat caacctctac cgccaacgct accaggtgcc gcatccgctg
     1021 cgcttccata gcaccgccgg taaccgcatc gacgaggtgg gccgctacca gcgctaccgc
     1081 gaccgccccg acacgccgcg cgccaccgat ggcctcaaga tcctgctggt gggcgaactg
     1141 gcctataacg ccgaccgcgt gctggcactg gaggagcgcg gccacaagct ctacggcctc
     1201 tggatgcccg acccctactg gtacaacacg gtggggccgc tgcccttcgg ccacgtcgaa
     1261 gacctgcctc gcgacgactg gcgcgccgcg atccgtcgcg tccaacccga cgtgatctac
     1321 gcgctgctca actggcaggc cgtgccgttc gcccacgagg tgctgcgcgc cggcctgggc
     1381 gtgcccttcg tctggcattt caaggagggg ccgttcatct gcctggagaa aggcacctgg
     1441 aacgaactga tcgacctcta cacgcgggcc gatgggcgca tctactccag ccccgagatg
     1501 ggcgattggt tcgagacggt ggtgccgggc atcgggcgcc tggggccggc gctcgtgctc
     1561 gacggcgacc tgcccaagcg cgactggttc accgccccgc cctcatcccg cctctcggac
     1621 gcggacggcc agttgcacac ggtggtgccg ggccgcccta tcggcctgca cccgcatacc
     1681 gtgggcgaac tggccgccga gggcgtccat ctgcactttt acggagactt cacccacggc
     1741 cagtggaaag cctggatcga gaaggcgcgg ggtatcgcgc ccgcccatct gcacctgcac
     1801 gccaacgtgg accaggagga ctgggtgacg gagttctcgc agtacgacgc gggctggctg
     1861 cacttcttcc agagcgagaa cgcgggcgac atccggcggg ccaactggga cgacctgaac
     1921 tacccggcgc gcatcgccac gctggccgtc gcgggcctgc ccctgttgca gggcgacaac
     1981 gctggcgcca tcgtcgccac gcaaaccctg gcccgcgaac gtgacctggg tctgttcttc
     2041 cgcgacatgg ccgaactggg cgcccaattg cgcgaccgcg cccgtctggc gcagatccgc
     2101 gccaatgtct ggcggcagcg cgacgagttc accttcgacc accacgccga ccggctgatc
     2161 gccttcttcc gcgaggtgat agctcaaggc gccaggtagc cgcccccgct aattccgctc
     2221 tgtaggggcc ggtttgaaac cggctcggcg tccggggggc ggtgtagggg ccggtttcaa
     2281 accggcccgg cgtccgccgg cggcgcggca tcccccgcgg gcaccagggc gaaggccagg
     2341 gccagcaggg ccgggacgta gagatagggc cagatcaccg ggttgaccac catgaacagc
     2401 aggtaggcgg cggcgcccca gcgcagcgcg gcggcgggcg tgcgcaccgg gcgcaggcgc
     2461 agcgccagca ggatcacacc cagcccgccc accaggacaa tttgcagcgg cttgagccag
     2521 tcttgcaggc tgttgtacca gaacggcccg gccagcccca cctccagcgc ccagcttttg
     2581 tcggtctgcc acttgagcag cggcaggcga ttgatgtcgt tgaaccagcc gatggcgccg
     2641 tcgaggaagc cgcgccgccc ccagagcagg aacggcccga ccagcacggc gaacaccacc
     2701 cccgctacca ccacccgccg caacgccacg cgccacccct gcgccagcca cgacgcgccg
     2761 aggaagggcg cgaagggcag ggccagcggg gcggcggcca gggccgcgcc cagccccgcg
     2821 ccgggcagca gccagccggg ccgggcgcgc gccgcaacca ccgccgtggc gaaggccacg
     2881 cagagccaca gccagtaggg cggggcggcg gtgctgatgt cccactggaa caagccgggc
     2941 gtgaggtaga gcaggccgaa ggcatgggcc gccggaccgg gaccgcgccg cccgcccgcc
     3001 agcaacagca gcgcgcccac cagcgggccg aacagcaggt tgacccagcg caggtcgatc
     3061 ccggccaggg cggcaggcag gtagctgagc acggtcagcg gccagtaggt cagcggcagg
     3121 tcccacggca tctggtagac gatgtagggg ttttgcgcct ggaggatgcg cgcggtggac
     3181 atttgcagca ggggtaacat atcccctaag cggatggtca gcgggataac ggcgatgccg
     3241 atcagccgca gggctagccc ggccccgacg gcgacggcca ggaaccgcgc cgggcgcagc
     3301 agcaccagc
//
LOCUS       DASUYC010000866         2068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1831948, whole genome shotgun sequence.
ACCESSION   DASUYC010000866 DASUYC010000000
VERSION     DASUYC010000866.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1831948"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /gene="gpmI"
                     /locus_tag="VF276_14210"
     CDS             complement(<1..793)
                     /gene="gpmI"
                     /locus_tag="VF276_14210"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEX6608055.1"
                     /translation="MAVPKPVVLIIMDGWGINPNPRANAVAQAATPTLDRLYATCPNT
                     ALDASQQDVGLPDGQMGNSEVGHQNMGAGFVVYQELTRLDRAIADGSFFENPVLVGAC
                     EHVKARNSTLHLFGLLGPGGVHSHWAHLYALLEMAKRRGIERVVYQAFTDGRDTPPQS
                     GLGFMQNVLENMDRIGVGRVGTVSGRYYAMDRDNRWDRVQKAYDAIVKGEGRHADTPL
                     AAFQLSYDNGVTDEFIVPTVIDGPEPAPRLSDGDAVIYFNFRGDRG"
     gene            1161..>2068
                     /locus_tag="VF276_14215"
     CDS             1161..>2068
                     /locus_tag="VF276_14215"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HEX6608056.1"
                     /translation="MAGGTREILVLNAGSSSLKLGLFDWETEEQLADKGVDWSHGHEG
                     GVSTHGDALRFLLQEYDLDAVAAVGHRVVHGGTRYQQAVRIDADVKATIADLARLAPL
                     HNPAALQGIEAVDDLLPDVPQVAAFDTAFHSTMPPTGYLYAVPWQWTEEWGIRRFGFH
                     GLSHAYCSGRALEMLGRPAPGSRLVTCHLGNGCSLAAVQDGRSVATTMGFTPLEGLMM
                     GTRSGSIDPGLLIHLLAHNYVDVPGLDDALNHESGLKGVSGLSGDMRDIQAAVGRGDA
                     RAQQAFALYNARIREGIAAMVAAMNGV"
BASE COUNT          322 a          710 c          700 g          336 t
ORIGIN      
        1 ggccccggtc gccacggaaa ttaaagtaga taacggcgtc gccgtcgctc aggcggggcg
       61 cgggttccgg cccgtcgatc acggtgggca cgataaactc gtcggtcacg ccgttgtcgt
      121 aggaaagctg gaaggcggcc agcggcgtat cggcgtggcg gccctcgcct ttgacgatgg
      181 cgtcataggc cttctgcacg cgatcccagc ggttgtcgcg gtccatggca tagtagcgcc
      241 cgctgaccgt gcccacgcgg cccacgccga tccggtccat attctccagc acgttttgca
      301 tgaagcccag cccgctctgc ggcggcgtgt cgcgcccgtc ggtgaaggcc tggtagacca
      361 cgcgctcgat gccccggcgc ttcgccatct ccagcagggc gtacagatgc gcccagtggc
      421 tgtgcacgcc tcccggcccc agcaggccga acaggtgcag cgtgctgttg cgcgccttga
      481 cgtgctcgca ggcgcccacc agcaccgggt tctcgaaaaa cgagccgtcg gcgatggccc
      541 ggtcgagccg cgtcagttcc tggtagacga cgaacccggc gcccatgttc tggtgcccca
      601 cctcggagtt gcccatctgc ccgtcgggca ggccgacatc ctgctgcgag gcgtcgagcg
      661 cggtattcgg gcaggtggca tagagccggt ccagcgtcgg ggtggccgcc tgcgccacgg
      721 cgttggcgcg cgggttcggg ttgatgcccc agccatccat gataatcaac acgacaggtt
      781 ttggtacagc catgatcttc cgtctccttt tggtccgcga ttcgagcctg gcccgccatt
      841 tgcggcgccc tggcccctcc tgcgattata tcagcatccg ggccacaggt gcagcacgct
      901 ccattatagc cccacccggt aagcagccgg gtaaaaatac atgacaattg ggcgacgggc
      961 gcgcccggag gctcaagcct ccggctagcg cgacgaaggc cgcctgcgcg gcctctttgc
     1021 ctagcccgcg caggcgggct tcgtagacgt tgcccgcccg tccacgggcc gggcgatgcc
     1081 gccgcattgg cggggcgggg cggttggtgc tatactcctg gcagggtccg gacttcttgg
     1141 ggaagcagag gtattggcat atggcaggtg gaacacgcga gatcctggtt ctgaacgccg
     1201 gttccagcag cctcaaactc ggtctgtttg actgggaaac cgaggagcaa ctggccgata
     1261 aaggggtcga ttggagccac ggccacgagg gtggcgtcag cacacacggc gatgccctgc
     1321 gcttcctgtt gcaggagtac gacctggacg ccgtggccgc ggtgggccac cgggtcgtcc
     1381 acggcggcac gcgctaccag caagcggtgc gcatcgacgc cgacgtgaag gccacgatcg
     1441 ccgacctggc gcggctggcg ccgctgcaca accccgccgc gttgcagggc atcgaggcgg
     1501 tggacgacct gctgcccgat gtgccccagg tggccgcgtt cgacacggcg ttccacagca
     1561 ccatgccgcc caccggctac ctctacgcgg tgccctggca gtggaccgag gagtggggca
     1621 tccgccgctt cggcttccac ggcctcagcc acgcctattg cagcgggcgc gcgctcgaaa
     1681 tgctggggcg cccggcgccg ggcagccgcc tcgtcacctg ccacctgggc aacggctgct
     1741 cgctggccgc cgtgcaggat ggccgctcgg tggcgacgac catgggcttc accccgctcg
     1801 aaggactgat gatgggcaca cgctccggct ccatcgaccc agggctgctg atccacctgc
     1861 tggcccacaa ctacgtcgat gtgccgggac tggacgacgc gctcaaccac gagtcggggc
     1921 tgaaaggcgt ctccggcctg tcgggcgaca tgcgcgacat ccaggccgcc gtcgggcggg
     1981 gcgacgcccg cgcgcaacag gcgttcgcgc tctacaatgc ccgcatccgc gagggcatcg
     2041 cggccatggt cgcagccatg aacggggt
//
LOCUS       DASUYC010000867         2444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1064403, whole genome shotgun sequence.
ACCESSION   DASUYC010000867 DASUYC010000000
VERSION     DASUYC010000867.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064403"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1596)
                     /locus_tag="VF276_14220"
     CDS             complement(<1..1596)
                     /locus_tag="VF276_14220"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEX6608057.1"
                     /translation="MTTASPTAAETAAPAPDARPTLMLVDGYALIYRAYHALPANMMT
                     KDGEPTNAILGFTTMLLDTLRKEKPEYLAVAFDRGRTFRHDLSPEYKATRSAMPDDLR
                     GQIKRVRELLDALKTPIFEQEGFEADDVIGTLARQAEGQGIRTLIVTGDMDELQLVTG
                     ATEVVTPTGRNRFNETKLYDVPAVEERYGFGPLLIPDYKALIGDKSDNIPGVPGIGEK
                     TATDLIKQYGTLEAMLDHLDEIKPPKAQKALREHAEQARQSKHLATIVRDVPVTLDLT
                     YCRAGDYDRDRVVDLFRALEFRNLINKLPNAPAPPVRVAPGAGRPGPGGPVQQLALFT
                     DEAAVEAMAAVDAAADQGTMPPGYQLVTTREALDALAKRLQEVDRFAFDVESTSKEEM
                     LADLVGLSVAPAAGESYYVPVAHVAPPGQPAPEQVSLDDLRAALGPIFAATHPLKDAH
                     NEKYDMMVVERAGMPVNEAGIGVDTMIVAYLLGERSKGLKELAFTRLGVEMTPITHLI
                     GSGRGQLTFDQVDVVKGGAYAAAD"
     gene            complement(1809..>2444)
                     /locus_tag="VF276_14225"
     CDS             complement(1809..>2444)
                     /locus_tag="VF276_14225"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX6608058.1"
                     /translation="GDIIKTVNGQEAGSTLTFAQAMDRVKSGTTTITVERNGAPVTLT
                     AEVANSGLDGWSYRLPTHVVTYPPLESIGRGFSRTGDLLRRIPEGLATVVAGLFSGQS
                     EAGVLSGPVGIAQLTGEVATEQGISGLVTLTALLGINLFLINLLPLPALDGGRLLFIL
                     VELLRGGRKIAPEKEGMVHLIGMGVLLLLMVIITFFDVQHLLEGGSILHPR"
BASE COUNT          391 a          807 c          824 g          422 t
ORIGIN      
        1 gtcggcggcg gcataagcgc cacccttgac gacatccacc tggtcgaagg tcaactggcc
       61 gcgcccgctg ccgatgaggt gggtgatcgg cgtcatctcc acgccgagcc gcgtgaaggc
      121 cagttccttg aggcccttgg accgctcgcc caggaggtag gccacaatca tcgtgtccac
      181 gccgatgccg gcctcgttca ccggcatgcc tgcgcgttcc acgaccatca tatcgtactt
      241 ctcgttgtgg gcgtccttca gcgggtgcgt ggcggcgaag atcgggccga gcgcggcacg
      301 caggtcgtcc agcgacacct gctccggcgc cggctggccg ggcggcgcga catgggccac
      361 cggcacataa taggactcgc ccgccgccgg ggccaccgac aggcccacca ggtcggccag
      421 catctcttcc ttgctggtgc tctccacgtc gaaggcgaag cgatccacct cctgcagccg
      481 ctttgccagc gcgtccagcg cctcgcgggt agtgacaagc tggtagccgg gcggcatcgt
      541 gccctggtcc gccgctgcgt ccaccgcggc catcgcttcc accgccgcct cgtcggtgaa
      601 cagagcgagc tgctggacgg ggccaccggg gccgggccgc cccgcgccgg gggccacgcg
      661 cacggggggt gccggggcgt tcggcagctt gttgatcagg ttgcggaact ccagcgcacg
      721 gaacaggtcc accacgcggt cgcggtcgta atcgccggcc cggcaatagg tcaggtcgag
      781 cgtcaccggc acgtcgcgca cgatggtcgc caggtgcttg ctctggcgcg cctgctcggc
      841 gtgctcgcgc aacgccttct gcgccttggg cggcttgatc tcgtccaggt ggtcgagcat
      901 cgcctccagc gtgccgtatt gcttaatcag gtcggtggcc gttttctcgc cgatgccggg
      961 cacgccgggg atgttgtcgg acttatcgcc gatcagcgcc ttgtaatcgg ggatcagcag
     1021 cggcccgaag ccatagcgct cctccacggc agggacatcg tagagcttgg tctcgttgaa
     1081 gcggttgcgc ccggtgggcg tcaccacctc agttgcgccg gtgaccagtt gcaactcgtc
     1141 catgtcgccg gtcacgatca gcgtgcggat gccctgcccc tcggcctggc gggccagcgt
     1201 gccgatcacg tcgtcggcct caaagccctc ctgctcgaaa atcggagtct tgagcgcgtc
     1261 gagcaactcg cgcacacgct tgatctggcc gcgcaggtcg tcgggcatcg ccgaccgggt
     1321 cgccttgtac tcgggcgaca ggtcgtggcg gaaggtgcgc ccgcggtcga aggccaccgc
     1381 caggtactcc ggcttctcct tgcgcagggt atcgagcagc atggtggtga agcccaggat
     1441 ggcgttggtc ggctccccgt ccttcgtcat catgttcgcg ggcaaggcat gataggcccg
     1501 ataaatcagg gcatagccgt caacgagcat caaggtcggg cgcgcgtcgg gcgcgggcgc
     1561 agcggtctcg gcagccgtgg gggaggcagt agtcatgcat ccattataag cgttttgcgg
     1621 ccgcggcgca aaaggaatcg ggccgattcg cggtcggtaa ttagaactaa tgttcgtgcg
     1681 gctgcggatg gaggcggcga ataatccgca aatcccggcg cggtgcgccc tgattgcagc
     1741 aaccgggcaa agaaaagcgc ctgctccgta gagggaagca ggcgccttgt cacacggagt
     1801 gattatgcct accgggggtg caggatgctg ccgccctcca gcagatgttg cacgtcgaag
     1861 aaggtgatga ttaccatgag caggagcagg acgcccatgc cgatgaggtg gaccatgccc
     1921 tctttttcgg gcgcgatctt gcgcccaccg cgcagcagtt cgaccaggat aaacagcagg
     1981 cgccctccgt ccaaagcggg gagcggcagc aaattgatca ggaacaggtt gatgccgagc
     2041 agcgcggtca gcgtgaccag accgctgatg ccctgctccg tcgccacttc gcccgtcaac
     2101 tgtgcgatgc ccaccggccc ggagagcacg ccggcctccg actggccgga gaacagtccg
     2161 gcgaccacgg tggcgaggcc ctccggaatg cgccgtagca gatcgcccgt gcggctgaag
     2221 ccccggccaa tggattccag cggcggatag gtgacgacgt gcgtgggcag ccggtagctc
     2281 cacccatcca gtccgctgtt ggcgacctcg gcggtcagcg tcaccggcgc gccgttgcgc
     2341 tccacggtaa tcgtggtggt gccgctcttg acccgatcca tggcctgagc gaacgtgagc
     2401 gtgctgcctg cctcctggcc gttcacggtc ttgatgatat cgcc
//
LOCUS       DASUYC010000868         2237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1545414, whole genome shotgun sequence.
ACCESSION   DASUYC010000868 DASUYC010000000
VERSION     DASUYC010000868.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1545414"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..546
                     /locus_tag="VF276_14230"
     CDS             220..546
                     /locus_tag="VF276_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608059.1"
                     /translation="MGSAIFGWLVVVLVGIEFGVVAYVRRNRYPRFRFSIWAPAIYLA
                     MIAGDFLLAALLSALLGAPTADVTNGRTTLLTGALIAGLLAGAVALLTLFFQWVLRTN
                     ITDPPE"
     gene            complement(553..>2237)
                     /gene="murJ"
                     /locus_tag="VF276_14235"
     CDS             complement(553..>2237)
                     /gene="murJ"
                     /locus_tag="VF276_14235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX6608060.1"
                     /translation="AAGDLAVPPRMLARRLDTRDLPAEAAAAPAPARHPTARRLAGAT
                     ALVGGAFIISRLLGLFRDQVIAAHFGTSDALDTYIAAFRVPDTLFLLIIGGAVGSAFI
                     PVFSGLLEQQKTAAAWRLTSTMINASVVLLSLGGILLGLLAPWLVALIIAPGWAPEKQ
                     AATVELTRIMLLSPLFLGLGGWAQGILNARHSFTLPALAPIAYNGAIIAGALFLTPLW
                     GVRGLALGVVIGAALHFLVQLPGLVRAGMQYRPLHLDIHDEGAAQVGRLMGPRIVGQA
                     AFQANIVVVTSLASFLPEGQLSAFNYAYLLMMLPYGVFAMSLATVLFPTMTAQVARGD
                     LDAMRTTLGGGLRTLVFLTLPSAVILGLLRYQIVGLLFQFGAFHQGSTELVAAALGWF
                     AWGLLSYSVVELLTRAFYALHDTRTPVIVSIVTVALNMGLSWALAIGLHMQHTGLALS
                     LAATTTVELAGLLFFLRRKLQGVFDRAALRAFGVSLLGAGLMAAVLWPLVPFLATNIW
                     AYPGDTAGQKVATALVLGIGCLVGGGIYLAVARVFHAAELGQALRMLRRRAS"
BASE COUNT          395 a          752 c          794 g          296 t
ORIGIN      
        1 caaatgttcc tctcccatta tagtacagtt gttctattct gtcaataatc aaaaaagcgg
       61 aaaggcgatt gcgaggggtc gaggcatggg gtagaatgga cagacaagcg attcaggggg
      121 ccgatttgcc aacggcgcgc cggcctgttg gcggtgtagg ggccggtttc aaaccggccc
      181 gcgcgtgcgg cgttacggat ggcgacggta ggggaatcga tgggcagcgc gattttcggg
      241 tggctggtgg tggtcctggt cgggatcgaa ttcggcgtcg tcgcctacgt gcggcgcaac
      301 cgctacccgc gcttccggtt cagcatctgg gcgccggcca tctacctggc gatgatcgcc
      361 ggcgacttcc tgctggcggc gctgctatcc gccctgttgg gcgcgcccac cgcggatgtc
      421 accaacgggc gcaccaccct gctgaccggc gcgttgatcg ccgggctgct ggccggggcc
      481 gtcgccctgc tcaccctctt cttccagtgg gtgctgcgca ccaatatcac cgacccgccg
      541 gagtagatgt cactaggaag cccgccggcg caacatgcgt agcgcctggc cgagttccgc
      601 ggcgtggaag acgcgcgcca cggccaggta gatgccgcca cccaccaggc agccgatacc
      661 cagcaccagt gcggtggcga ccttctgccc ggcagtatcg cccggatagg cccagatgtt
      721 ggtggcgagg aacggcacga gcggccagag cacggcggcc atcagcccag cccccagcag
      781 gctgacgccg aaggcgcgca gcgcggcccg gtcgaacacg ccttgcagct tgcggcgcag
      841 gaagaacagc agcccggcca gttcgacggt cgtggtcgcg gccaggctca gggccagccc
      901 cgtgtgctgc atgtgcagcc cgatcgccag cgcccaactc aggcccatgt tcagcgccac
      961 ggtgacgatg gagacgatga ccggcgtgcg cgtgtcgtgc agcgcgtaga aggcgcgggt
     1021 gagcagttcg actaccgagt aggagagcag gccccaggcg aaccagccca gcgccgccgc
     1081 caccagttcg gtcgatccct ggtggaacgc gccgaactgg aacagcaggc cgacgatctg
     1141 gtagcgcagc aggcccagga tcaccgccga gggcagcgtt aggaacacga gcgtgcgcag
     1201 cccgccgccc agcgtcgtgc gcatggcgtc gaggtcgcca cgcgccacct gcgcggtcat
     1261 ggtggggaac aacaccgtcg ccaggctcat ggcgaagacg ccgtagggca gcatcatcag
     1321 cagataggcg taattgaagg cggacaactg gccttcgggc aggaacgagg ccagcgaagt
     1381 gacgacgacg atgttggcct ggaacgccgc ctggccgacg atgcgcggcc ccatcagccg
     1441 cccgacctgc gccgcgcctt catcgtggat gtcgagatgg aggggccggt actgcatgcc
     1501 cgcgcgcacc agccccggca actgcaccag gaagtgcaac gccgcgccga tcaccacgcc
     1561 gagcgccagc ccgcgcacgc cccagagcgg cgtcaggaac agcgccccgg cgatgatcgc
     1621 gccgttgtag gcgatgggcg ccagggcggg cagggtgaag ctgtgccgcg cgttgaggat
     1681 gccctgcgcc cagccgccca ggcccaggaa cagcggcgac agcagcatga tgcgcgtcaa
     1741 ttcgaccgtg gcggcctgct tctccggtgc ccagcccggc gcgatgatca gcgccacgag
     1801 ccacggcgcc agcagaccca gcaggatgcc gcccagcgac agcaacacca ccgaggcgtt
     1861 gatcatcgtg ctggtcagcc gccaggccgc cgcagtcttc tgctgttcca gcaggccgga
     1921 aaagaccggg ataaaggccg agccgaccgc cccgccgatg atcagcagga acagcgtatc
     1981 gggcacgcgg aaggcggcga tatacgtgtc gagcgcgtcg gaggtgccga agtgggcggc
     2041 gatcacctgg tcgcggaaca ggcccagcag gcggctgatg atgaacgcgc cgcccaccag
     2101 cgccgtggcc ccggccagcc gccgggccgt gggatgccgg gcgggcgcgg gcgcggcggc
     2161 agcctccgcc ggcaggtcgc gggtgtcgag ccggcgggcg agcatgcgcg gcggcacggc
     2221 gaggtcgccc gccgcag
//
LOCUS       DASUYC010000869         2305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1065198, whole genome shotgun sequence.
ACCESSION   DASUYC010000869 DASUYC010000000
VERSION     DASUYC010000869.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065198"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VF276_14240"
     CDS             <1..732
                     /locus_tag="VF276_14240"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEX6608061.1"
                     /translation="NGRFPDVDANGNAVHIVLNSNAGGAFDNYYTWTAAGSPPAPPPT
                     ATPAPPTATPVPPTPCTPGRFSDVPAGSPYYTQITDLVQRGAISGYSDCSFRPGANIT
                     RGQVAKVLVLGLGLRLVNPAQGHFTDVPPGSPFYTYIETAYARGIISGYADHTFRPGN
                     PVTRGQLAKMSVTGRGWTLSNPRTPTFSDVGRADPFYTYIETASRHGVISGYTCGTNC
                     LQFRPTAPAIRGQAAKVIDLAVTAR"
     gene            complement(862..2082)
                     /locus_tag="VF276_14245"
     CDS             complement(862..2082)
                     /locus_tag="VF276_14245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX6608062.1"
                     /translation="MTLLVGLGAGILLDREVLGAQSQAGPGADRPQSSTDLNYNLINQ
                     ALEIIDQNYVDRAAIQTQPLTYGAASGLVDALGDTGHSRFMTPDEVRQQQTLSTGQFE
                     GIGAEVQMKDGGVTIVAPYDGTPAQRAGLLPGDVIVQVEGKSVAGETLSEVVSKVLGP
                     AGTTVHLTILTPSTGNTRDVTIVRARITIHDVTWHQLPGTTLAHVRIAEFSKGVDRDL
                     DQVLATIQQQHLTGLILDLRNDPGGLLDESVATASNFLSSGNVLLEKNAKGQTTPVPV
                     EKEGTPVTLPMVVLINEGSASASEIVAGALQDAKRAKLVGETTFGTGTVLSTFPLEDG
                     SALLLATEEWLTPAGRVIWHHGITPDVVVTQTLSTLPVLPEAEQTLTPQELQNSGDAQ
                     LLRGISILASPPTP"
BASE COUNT          352 a          853 c          736 g          364 t
ORIGIN      
        1 aacgggcgct tccccgatgt ggacgccaac ggcaacgccg tgcatatcgt gttgaacagc
       61 aacgcgggtg gcgcgttcga caactactac acgtggaccg ctgccggcag cccgcccgcg
      121 ccgccgccca ccgccacgcc ggccccgccc acggcgaccc cggtgccgcc cacgccgtgc
      181 acgcccggtc gcttcagcga cgtgcccgcc ggctcgccgt actacaccca gatcaccgat
      241 ctggtgcagc gcggcgccat cagcggctac agcgactgca gcttccggcc cggcgcgaat
      301 atcacgcgcg gccaggtcgc caaggtgctg gtgctcggcc tggggctgcg gctggtgaac
      361 ccggcgcagg gccacttcac cgacgtgccg cccggctcgc cgttctacac ctacatcgag
      421 accgcctacg cccgcggcat catcagcggc tatgccgatc acacgttccg gcccggcaac
      481 ccggtgacgc gcggccagtt ggcgaagatg agtgtcaccg ggcgcggctg gaccctctcc
      541 aacccgcgca cgcccacctt cagcgatgtg ggccgcgccg acccgttcta cacctatatc
      601 gagacggcct cccggcacgg ggtgatctcc ggctacacct gcggcacgaa ctgtctgcag
      661 ttccgcccga cggccccggc catccgcggc caggccgcca aggtgatcga cctcgccgtc
      721 acggcccgtt aacggcggcg cggaccgggc gcctgcttta cgaggtgccc ggcctgccgt
      781 gaacagcaag cacgcccccg gtgcgaacgc tcgcaccggg ggcgtgccac atccgggcaa
      841 cgggcgccgt aaatggcgcc gctacggggt ggggggagac gcgaggatgc tgatgccgcg
      901 caacagttgg gcgtcgccgc tgttctgcaa ctcctgcggc gtgagggttt gttccgcttc
      961 cggcaacacc ggcagcgtgg acagcgtctg cgtcaccacc acatcggggg tgatgccgtg
     1021 gtgccagatc acgcggcccg ccggggtcag ccattcctcc gtcgccagca gcagcgccga
     1081 cccatcctcc agcgggaacg tgctcagcac cgtcccggtg ccgaaggtgg tctcgcccac
     1141 cagtttggcg cgcttggcat cttgcagcgc ccccgccacg atctcggacg cgctggcgct
     1201 gccctcgtta atcagcacca ccatcggcag cgtgacgggc gtgccctctt tctccaccgg
     1261 caccggtgtc gtctgcccct tggcgttctt ctccagcagc acattgccgc tgctcaggaa
     1321 gttgctggcc gtggccacgc tctcgtccag caggccgccc ggatcgttgc gcagatccag
     1381 gatgagcccg gtcaggtgct gctgctggat ggtcgccagg acctggtcga ggtcgcgatc
     1441 cacgcccttg ctgaactcgg cgatgcgcac gtgggctagc gtggtgcccg gcaactggtg
     1501 ccaggtcaca tcgtggatcg tgatccgcgc gcgcacaatc gtcacatcgc gcgtgttgcc
     1561 ggtggacgga gtcaggatgg tcagatgcac cgtcgtgccc gccggtccca gcaccttgct
     1621 caccacctcg ctgagcgtct ccccggccac cgacttgccc tccacctgca cgatcacgtc
     1681 gcccggcagc aaccccgcgc gctgggccgg ggtgccgtca taaggcgcga caatcgtcac
     1741 gccgccatcc ttcatctgga cttccgcgcc gatgccctcg aactggcccg tgctcagcgt
     1801 ctgctgctgg cgcacctcgt cgggcgtcat gaagcggctg tggccggtgt cgcccagcgc
     1861 atcgaccaac ccgctggcgg caccgtaggt caggggctgc gtctggatcg ccgcccggtc
     1921 cacgtaattc tggtcgataa tctccagggc ctggttgatc aggttgtagt tgagatcggt
     1981 tgatgattgg ggacgatccg cgcccggccc cgcttgcgac tgcgcgccca ggacctcccg
     2041 gtcgagcaaa atacccgccc ccaacccaac caggagcgtc aaaagaagca gacccgtaat
     2101 ccagacctgc gaccgtgggt gttgcatagc gcctccgaga gccgggggcc cggtcgatgc
     2161 ccggatatga ggcccggcgt atgcgcgacc gggtagccag gtcgcgcaat gaacgaggaa
     2221 agaaccgtcg atagcagcgc gggccgccgc cggtccgctg cccgattggc cgccgggcaa
     2281 ccgttgcacc cctgccgcta tcgca
//
LOCUS       DASUYC010000870         3639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1068935, whole genome shotgun sequence.
ACCESSION   DASUYC010000870 DASUYC010000000
VERSION     DASUYC010000870.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068935"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VF276_14250"
     CDS             complement(<1..492)
                     /locus_tag="VF276_14250"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX6608063.1"
                     /translation="MTCARYRALISRYLDDELTPRQRTELLDHVKHCASCAADLARYR
                     QAEVLLRKIPEGQPAPELKLAVLREARRRRRRERHSLIWLLSAGGRANPRIVACLLAI
                     GLLLPVLAGVLLPVASGQPIGARFGFSAPATPTSAYLDVPESPTPPPPGPLSPPHLVT
                     TKPY"
     gene            complement(489..1034)
                     /locus_tag="VF276_14255"
     CDS             complement(489..1034)
                     /locus_tag="VF276_14255"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6608064.1"
                     /translation="MDVAVIERARQGDEDAFAEIYEYYSAPIHRFIYRMLGSAEQADD
                     MTQETFIRAYQSLDRLGQDPNLSAWLYRIASNACMDALRRRKRFTWLPMFDRSDRDDE
                     FTTDDFTPQIAEADAVRRVLAEMPAPLSMTLVLRSLEGFSCEEIAEIMNVPKGTVFSR
                     LARAREQFARLYARAGREEKG"
     gene            1264..1659
                     /locus_tag="VF276_14260"
     CDS             1264..1659
                     /locus_tag="VF276_14260"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6608065.1"
                     /translation="MQAYVLVVDDDEAVRNFIASLLSEEGYPVRSAGDAYQAMDQVSA
                     APPGLLLLDLMMPGVSGAEFLARLRRDERGQDLPVILISAHPRLREIAQDLHVEAAIP
                     KPFDITSLLHHVSQVLGPPLPTADPAYGT"
     gene            1822..2793
                     /locus_tag="VF276_14265"
     CDS             1822..2793
                     /locus_tag="VF276_14265"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX6608066.1"
                     /translation="MADNRDAAVYNIKAVARLTGVAADTLRRWESRYNILSPQRSTGG
                     YRMYSQRDVDTIRWLKARVEEGLTISRACDLLRTRQDTDLSDGGGALSAGGALRGPAA
                     GAVSAGVRALPVLVDEMLTWLGAMDETRAGQVMTEALSLYTVEQVVEELVYPLLVEVG
                     ERWMRGDWSVAQEHFASAFMRGQLANLLHAGPHNAGGRLALVACAPEELHEIGALVIA
                     LYLRRNGYRVIYLGQNVPLDSLVSMVRTLRPAVVCCSAARPDTAANLRPLAGLIDELH
                     RQTGYRPVLVYGGLVFNRQPRLAEEMGGIYPGPAAGAAVARVRELTA"
     gene            complement(2783..>3639)
                     /locus_tag="VF276_14270"
     CDS             complement(2783..>3639)
                     /locus_tag="VF276_14270"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX6608067.1"
                     /translation="TAPWPLRLLDRPAANISQGRNTAIQAAAHEIIAATDAGVRLPPQ
                     WLAALVAPFEGPGGAGIDVAGGFFAPDPRTVFERAMGATVLPARTDIKPARFLPSSRS
                     VAFRKAAWAAVGGYPEWLDYSEDLVFDLALKARGCRFVFVPDALVLFRPRGSLPAFYL
                     QYYRYARGDGKADLWRRRHAIRYGTYLGGPILAVLGRRRPALWALLALGAAAYCATPY
                     RRLRPWLAGLAPGECARAVALVPIIRLTGDVAKMIGYPVGVWWRLCHRRAGPAPDPSP
                     GESPALKP"
BASE COUNT          578 a         1222 c         1261 g          578 t
ORIGIN      
        1 gtagggcttg gtggtaacca ggtggggcgg ggagagcggg ccgggcgggg gcggggtcgg
       61 gctttcgggc acgtccaggt aggccgaggt gggcgtggcg ggcgcggaga agccaaagcg
      121 cgccccgatc ggctgcccgg acgccaccgg taacagcacc ccggccagca ccggcagcag
      181 caggccaatg gccagcaggc aggcgacgat gcgcgggttg gcccggcccc cggcggagag
      241 cagccagatg aggctgtgcc gctcgcgccg gcgccgccgc cgggcttcgc gtagcaccgc
      301 caatttcagc tccggcgctg gttggccctc aggtattttg cgtaataaga cttccgcctg
      361 gcgatagcgg gccaggtcgg cggcgcagct cgcgcagtgc tttacatgat cgagtaattc
      421 ggtgcgctgg cgcggcgtga gttcatcgtc gaggtaacgc gaaatgaggg cgcgataacg
      481 cgcacaggtc atcccttttc ctctcggccg gcccgtgcat atagccgcgc gaattgttcg
      541 cgcgctcgcg ccaaccgact aaagaccgtg cctttaggga cgttcataat ttcggcgatt
      601 tcctcgcagg agaagccttc cagcgagcgc aacaccagcg tcatgctcaa cggggcgggc
      661 atctcggcca agacgcgacg caccgcgtcg gcttccgcaa tctgcggggt aaaatcgtcc
      721 gtcgtgaatt cgtcgtcccg atcggatctg tcgaacatcg gcagccaggt aaaccgcttg
      781 cgccggcgca gcgcgtccat gcaggcgttc gacgcgatcc ggtataacca cgcgctcaga
      841 ttcgggtcct gacccaaccg gtccaggctc tgataggcgc gaataaaggt ttcctgggtc
      901 atgtcgtcgg cctgctcggc gctgccgagc atgcggtaaa taaagcggtg aatgggcgcg
      961 ctgtaatact cgtagatctc ggcaaaggca tcctcgtccc cctgccgagc ccgttcgatg
     1021 acggccacgt ccatcgccaa ttctcctggc tactgcgttc aagcgtaagg aacccgcccg
     1081 ctcttctcgc tcgtcgctca atttcattat aggggcgcgc ccttgccgcg tcaaggtctg
     1141 ggcggcccgg attagccccc aatccgctaa gagactggtt cgtggctaat tcttgcgatt
     1201 ctgtctgatt accgggttct cgcactcttt ccctggcttg cgagatgtga taaactaggg
     1261 gctatgcaag cctatgtgct cgtcgtcgac gacgacgaag cggtccgcaa ctttatcgcc
     1321 agcctgctga gcgaggaagg ctaccccgtc cgcagcgcgg gcgacgccta tcaggcgatg
     1381 gaccaggtga gcgcggcgcc gccggggctg ctgttgctgg acctgatgat gcccggcgtc
     1441 agcggcgccg aattcctggc gcgcctgcgc cgcgacgaac ggggccagga tctgccggtc
     1501 atcttgatct cggcccaccc gcggctgcgc gagatcgccc aggatctgca cgtcgaagcg
     1561 gccatcccca agcccttcga catcaccagc ctgctgcatc atgtgagcca ggtgctcggt
     1621 cccccgctgc ccaccgccga tcccgcctac ggcacgtgac cccgccgtct ctcccccggc
     1681 gactcccgct gcgtgcacag atcggactgc tgggtctcgt ccgccgcaac tttagtaaaa
     1741 cccttgatca accttgaact acgtgctatg atacagggaa ggcaggcgcg cggcgcgtaa
     1801 ttcatcttgg ggggagagac cttggctgat aatcgggacg cggccgttta caacatcaaa
     1861 gcggtggcgc ggctgaccgg cgtggccgcc gatacgttgc gccgctggga gagccgctat
     1921 aacatcttgt cgccgcaacg gagcacgggc ggctaccgta tgtattcgca gcgcgatgtg
     1981 gatacgattc gctggctgaa ggcccgcgtg gaagaaggcc tgaccatcag ccgcgcctgc
     2041 gacctgttgc ggacgcgcca ggacacggac ttgtccgatg ggggcggggc gctgagcgcg
     2101 ggcggcgcgc tgcgcgggcc ggcggcgggc gcagtgtctg ccggggtgcg cgccctgccg
     2161 gtcctggtgg acgagatgct cacctggctg ggggccatgg acgagacccg cgccgggcag
     2221 gtgatgaccg aagcgctcag cctctatacc gtcgagcagg tggtcgagga actggtgtat
     2281 ccgctgctgg ttgaggtggg cgagcgctgg atgcgcggcg actggtcggt ggcccaggag
     2341 cacttcgcca gcgccttcat gcgggggcag ctggcaaacc tgctgcatgc cggcccgcat
     2401 aatgccggcg gccggctggc gctcgtcgcg tgtgcccccg aggaattgca cgaaatcggc
     2461 gcgctggtga tcgcgctgta cctgcgccgc aacggctacc gcgtgatcta cctgggccag
     2521 aacgtgccgc tggacagcct ggtcagcatg gtgcggaccc tgcgcccggc ggtggtctgc
     2581 tgctcggcgg cccggcccga taccgcggcc aacctgcggc ccctggccgg cttgattgat
     2641 gagttgcacc ggcagacagg gtaccggccg gtgctggtct atggcggcct cgtcttcaac
     2701 cggcagcccc gcctggccga ggagatgggc ggcatctacc ctggcccggc ggcgggggcc
     2761 gccgtggccc gcgtgcgcga acttacggct tgagggccgg cgattcgccc ggagacggat
     2821 ccggcgcggg cccggcccgc ctgtggcata gccgccacca cacgcccacc ggatagccga
     2881 tcatcttggc gacatcgccg gtgagccgga tgatcggcac gagggccacg gcccgcgcgc
     2941 actctcccgg cgcaagcccg gccagccagg gccggaggcg ccggtagggg gtcgcgcaat
     3001 aggccgccgc cccgagcgcc agcaatgccc acagcgcggg ccggcggcgg cccagcacgg
     3061 ccaggatcgg gccgccgaga taggtgccgt agcggatggc gtggcgcctg cgccagagat
     3121 cggctttgcc gtcgccgcgc gcgtagcggt aatattgcag gtagaacgcg ggcaggctgc
     3181 cgcggggccg gaacaagacc agcgcgtcgg gcacgaagac gaagcgacag ccccgcgctt
     3241 tcagcgccag gtcgaacacc aggtcctcgc tgtaatccag ccactccgga tagccgccca
     3301 ccgccgccca cgccgctttg cggaaggcca ccgagcggct cgacggcaaa aaccgcgccg
     3361 gcttgatatc cgtccgcgcc ggcaggacgg tggcgcccat ggcccgctcg aagacggtgc
     3421 gcgggtcggg cgcgaagaac cccccggcca cgtcgatgcc cgcgccgccc ggcccctcga
     3481 acggcgcgac cagcgccgcc agccactgcg gcggcaagcg cacgccggca tcggtggccg
     3541 cgatgatctc gtgcgccgcc gcctggatcg ccgtgttgcg cccctggctg atgttcgcgg
     3601 cgggccggtc cagcaagcgc agcggccagg gcgccgtgg
//
LOCUS       DASUYC010000871         3956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_602526,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000871 DASUYC010000000
VERSION     DASUYC010000871.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602526"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1434)
                     /locus_tag="VF276_14275"
     CDS             complement(<1..1434)
                     /locus_tag="VF276_14275"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6608068.1"
                     /translation="MAPEPVGGPKTPRLFASKTRIPLPPPHFLSRPRLLATLRAGLER
                     KLTVVIAPAGFGKTTLVAELAREVGSAAAWLTLDPSDHDLSIFAYYLVDAIAQARPGF
                     GSATRSWLAATPAPATQIEELAALLIGELDDLGGDGLVLFLDDFHEVSDSDSITRLVD
                     VIVRYLPPHIRVVLSARRPPALTTTRLLVQQQISGVGTDDLRFNLAETETWLAGRPVP
                     ADPASGRQLVESTEGWVTGMILQAAVTVPAQPGATSEQLYAYLSSEVLERQPPAIQEF
                     LLRAAILTHLTVALCAAVLEVADAASTLRWIHDQQLFLTAVGGRDAPHYRLHQLFREF
                     LAARLRATDPAEFARLHHRAAAYFERHGDPGQAMGHLFAVQAWDAAAALAARVGAGEI
                     GAGRVERVERWLANFPEAERLARPALLLLETRVLSAQSQYVKAGELLARAERLFQEAG
                     DRPGLAEVLALRARLATARSDGRQMVGF"
     gene            complement(1988..3493)
                     /locus_tag="VF276_14280"
     CDS             complement(1988..3493)
                     /locus_tag="VF276_14280"
                     /inference="COORDINATES: protein motif:HMM:NF025067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HEX6608069.1"
                     /translation="MAPVQAPARILWVALALGWATDILFYGKPLGISVPLFVLLGLGA
                     LIGLGRREGVGPRRANLWLLAPIAFFAAMIFVRANTTLTVLNSLALLGLLGLLVYFYA
                     GGRLGRLGLLGYPTVLGLVGKHVVLDPVAPVAGGCEAVRPNPRTWRRAAPVLRGVALA
                     GPVLFVFAALLASADSVFAGLLGDLLQLNFLTSLPELLWRLALIGGAAWIVAGGLFYA
                     LRRRRAPDDPAPGAYRPGVSLGFVEGATVLALVNALFLVFNWIQVTYLFSGEAARTLD
                     YEAYREYVRRGFGELLVVAVLTMILILGLRWAAWKETPREGRILNGLSTLMIGLALLL
                     LGSAFMRMVVWEDITYYINTELRIYVRWFIVWLGVAFLWLLGTLWTRPERFAIGGFVV
                     ALGFLVSINLVNPDADVAAYNLARNDELSTRYLYLLSADAVPALAGGLDRTTGEVQQR
                     LRDDLARRLWTMEHDPTWQAWPAFHLARWDAYHTLTALQAAGKLDTISATR"
     gene            complement(3815..>3956)
                     /locus_tag="VF276_14285"
     CDS             complement(3815..>3956)
                     /locus_tag="VF276_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608070.1"
                     /translation="RSLALREFALGLEALERFVRREPLRRTHECVFGVLALESEPVDP
                     RL"
BASE COUNT          666 a         1341 c         1429 g          520 t
ORIGIN      
        1 aaacccgacc atctggcgcc cgtcactgcg ggcggtagcc agccgggcgc gcagggccag
       61 cacctcggcc aggcccggcc ggtcgcccgc ctcctggaac agccgctcgg cgcgtgccag
      121 caattcaccg gccttgacat attgcgactg ggcggacagc acgcgcgtct ccagcagcag
      181 cagggcgggc cgcgccaggc gctcggcttc cgggaagttc gccagccagc gctccacgcg
      241 ctccacgcgc ccggcgccga tctcgccggc cccgacacgg gccgccaggg ccgctgccgc
      301 atcccacgcc tgcacggcga acaggtggcc catggcctgg ccgggatcgc cgtggcgctc
      361 gaagtaggcc gccgcgcggt gatgcagccg ggcgaactcg gcgggatcgg tcgcgcgcag
      421 ccgggcggcg aggaactcgc ggaacaattg atgcagccgg tagtgcggcg cgtcgcggcc
      481 ccccaccgcg gtgaggaaga gttgctggtc gtgaatccag cgcagggtgc tcgcggcgtc
      541 cgccacctcc agcaccgccg cgcacaacgc cacggtcagg tgggtcagga tggcagcgcg
      601 cagcaagaac tcctggatgg ccggcggctg ccgttcgagc acttcgctcg acaggtaggc
      661 gtagagttgc tcggaggtgg cgccgggctg cgcggggacg gtcacggcgg cctgcaggat
      721 catgcccgtg acccagccct cggtgctctc caccaactgc cgcccggatg cgggatcggc
      781 gggcaccggg cgcccagcca gccaggtctc ggtctcggcc aggttaaagc gcaaatcgtc
      841 ggtgcccacg ccgctgatct gctgttgcac cagcaggcgc gtggtggtga gggccggggg
      901 tcgccgcgcg ctgagcacca cgcggatgtg cggcggcagg tagcgcacga tcacatcgac
      961 cagccgggtg atgctgtcgc tgtcgctgac ttcgtggaaa tcgtcgagga acaggaccag
     1021 cccatcgccg ccgaggtcgt ccaactcgcc gatgagcagg gcggctagct cctcgatctg
     1081 cgtggcgggc gccggagtcg ccgccagcca ggagcgcgtg gccgagccga agccagggcg
     1141 ggcctgggca atcgcgtcca ccaggtagta ggcgaagatc gacaggtcgt gatcgctggg
     1201 gtcgagggtc agccaggcag cggcagatcc cacctcacgg gccagttcgg cgaccagcgt
     1261 ggtcttgccg aagccggccg gggcaatgac cacggtcagt ttgcgttcga gacccgcgcg
     1321 cagggtcgcc aataaccgcg gccggctcaa gaagtgcggc ggcggtaacg gtatgcgagt
     1381 cttcgaggca aacagccgcg gcgttttcgg cccgcccacg ggttcgggcg ccatgcttca
     1441 ccgctttcct gaggaaccgc tccgacggtc gcgggcgcgc gccacaacgc gccggtgatg
     1501 gccacccgcc ggaccggctc ctgagcctgc caagtgctgc ccatattata gtaggcccgc
     1561 gccaaaaaca caaatgcgcc taacgagtgc tgagtgctga gtgacgacga gtggtcggtg
     1621 gtcagtggtc agtacgacga gtggtcagtg gtcagtggtc agtggtcagt acgacgagtg
     1681 atcggtggcc gcggtcagcc gtcagccgtg tgctacgcac cgctgcgcga acagcggtca
     1741 gccgtcagcg gtcagccgtc aggagacgcg tgaccgccga gtgccggcca cctggcgggt
     1801 ttgtcatgct gaacgcagtg aagcatctcg ccttgtacca tacgccgacg agactcttcg
     1861 ttgcgctcag agtgacaagg ccgggccgtc gcacgtctcc tgacggctga ccgctgacgg
     1921 ctgaccgctg ttcgcgcagc ggtgcgtagc aatagctgac cactgatcac tgaccactga
     1981 ccactcgtca ccgcgtggcg gagatcgtgt cgagcttgcc ggcggcctgt agggccgtga
     2041 gcgtgtgata ggcgtcccag cgggccaggt ggaaggcggg ccaggcttgc caggtcgggt
     2101 cgtgttccat ggtccagagg cggcgggcga ggtcgtcacg caggcgctgc tgcacctcgc
     2161 ccgtggtgcg gtcgaggccc ccggccagag cgggcacggc gtcggcggag agcaggtaga
     2221 ggtagcgggt cgagagttcg tcgttgcggg ccaggttgta ggccgccacg tcggcgtcgg
     2281 ggttgaccag gttgatgctg accaggaagc ccagcgccac gacgaagccg ccgatggcga
     2341 agcgctcagg gcgcgtccag agcgtgccca gcagccacag gaaggccaca ccgagccaga
     2401 cgatgaacca gcgcacgtag atgcgcaact cggtattgat atagtaggtg atgtcttccc
     2461 agaccaccat gcgcatgaag gccgagccca gcagcagcaa ggccagcccg atcatcagcg
     2521 tgctcaggcc gttgaggatg cggccctcgc gcggcgtctc cttccaggcc gcccagcgca
     2581 gccccaggat gaggatcatg gtcagcacgg ccacgaccag cagttcgccg aagccccgcc
     2641 gcacatactc gcggtaggcc tcgtagtcca gcgtgcgcgc ggcctcgccg ctgaacaggt
     2701 atgtgacctg gatccagttg aagaccagga agagggcgtt gaccagggcc agcaccgtgg
     2761 cgccttcgac gaagccgagg ctgacgccgg gccggtaggc gccgggggcc gggtcgtccg
     2821 gggcgcggcg gcggcgcagg gcatagaaca ggccgcccgc gacgatccag gcggccccgc
     2881 cgatgagcgc cagccgccag agcagttcgg gcaggctggt gaggaaattg agttgcagca
     2941 gatcgcccag cagcccggcg aagacgctgt cggcggaggc gagcagggcg gcgaagacga
     3001 acaacaccgg ccccgccaga gccacgccac gcagcacagg cgcggcccgg cgccaggtgc
     3061 gcggattggg gcgcaccgcc tcgcaccccc cggccaccgg cgccaccggg tcgagcacta
     3121 cgtgcttgcc gaccaggccg agcacggtcg ggtaaccgag cagccccagc cgccccaggc
     3181 gcccgcccgc gtagaagtag accagcagcc ccagcagacc gagcagcgcc aggctgttga
     3241 gcacggtcag cgtggtattg gcgcgcacga agatcatcgc ggcgaagaag gcgatgggtg
     3301 ccagcagcca gaggttggcc cgccgcggcc ccacaccctc acgccggccc aggccgatca
     3361 gggcgcccag ccccagcaac acaaacagcg gcaccgagat gccgagcggt ttgccgtaga
     3421 agaggatgtc ggtcgcccaa ccgagggcca gggcgaccca cagaatgcgc gcgggtgctt
     3481 gcacaggagc catggtcgat cctttcgggc cgggcgccgg gcaggggcag tccgccaacg
     3541 gcgcgccgcg ggtggtattc atcctcctaa cgcgcgactc cggtggcggt tgcggacggg
     3601 ccgtcagccg tcagccgtca ggagacggac gacggtcggt ccacggacac ccggccgcag
     3661 gatcgtccgg cccgtcaacg ggcgggctac gtcaactaag gccgcctgcg cggcctggta
     3721 tttcccccgt ggaccagccc gccgcggcgg gcttcgtcgc gctagcccgc tcctttagga
     3781 gccgggcgat ctcgctaccg ggcgggggtg accgctacag gcgcggatcg accggctcgc
     3841 tttcgagggc caggacgccg aagacgcact cgtgggtgcg gcgcagcggt tcgcggcgga
     3901 cgaagcgctc cagcgcctcc aggcccaggg cgaactcgcg cagggccagc gaccgc
//
LOCUS       DASUYC010000872         4231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1799762, whole genome shotgun sequence.
ACCESSION   DASUYC010000872 DASUYC010000000
VERSION     DASUYC010000872.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1799762"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..848
                     /locus_tag="VF276_14290"
     CDS             66..848
                     /locus_tag="VF276_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015204246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6608071.1"
                     /translation="MQLEGQVALITGAGSGIGKAAALLLAREGAKIGVLSRGAEEVEA
                     VAKEIEQAGGAALALVADISQPGEMQQAVKQLADRWGRIDIVFANAGINGVWAPLEEL
                     TPEDWDHTLSINLKGTFLTVKYAVPYLKKQGGSVIVTASVNGTRIFSNTGATAYSCSK
                     AGQVAFTKMTALELAPDKVRVNVICPGAITTQIDENTEKRDLEDVRIPVKYPEGSQPL
                     TGGNPGTAEQVAQLVLFLASDASSHITGTEVWIDGAESLLRG"
     gene            1026..1751
                     /locus_tag="VF276_14295"
     CDS             1026..1751
                     /locus_tag="VF276_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019488857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6608072.1"
                     /translation="MADMESTQRDGLGDVKRWVAVAAGGGLTIYGLSRRSLGGVALAV
                     AGGGLLARGLLGGQGGAESVDDEWETGRERRQAAAQEAGAVHVEKAITIEKSPAELYQ
                     FWRDFENLPRFMDNLESVTMAGDGRSHWVAKAPLGSHVEWDAEVTADEPNERIAWSSV
                     AEADVQNAGEVRFVPAPGDRGTEVHVRLDYKPPGGKAGAMVAKLFGDEPGQQVGDDLR
                     RLKQMLEAGEVATTAGQPSGRPM"
     gene            1868..3037
                     /locus_tag="VF276_14300"
     CDS             1868..3037
                     /locus_tag="VF276_14300"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010995071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HEX6608073.1"
                     /translation="MKAVCWWAANDVRVDTVPDPKIINPHDAIVQITSTAICGSDLHL
                     YDGYIPTMLKGDILGHEFMGEIVEVGSEVENLKVGDRVVVPFPISCGQCHYCRKGLWS
                     LCDNTNPKAALAEGMYGYSPAGIYGYSHLLGGYAGGQAEYARVPFADVGPIKIPAGLT
                     DEQVLFLSDIFPTGWMAAENCGIQPGDVVAVWGCGPVGQFAVKSAYLQGAERVIAIDR
                     IPERLKLAETAGGAEVINYVGTDVVEELKQRTGGRGPDACIDAVGMEAHGTGPLWYYD
                     RVAQATRMESDRPLVIREAIQACGKGGTVSLAGVYGGVVDKIPFGAAFNKGLTLKMGQ
                     THVQRYLQPLLDRIVAGDIDPSFVITHRLALDEAPEGYKIFKEKEDNCIKVVLKP"
     gene            3069..4112
                     /locus_tag="VF276_14305"
     CDS             3069..4112
                     /locus_tag="VF276_14305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6608074.1"
                     /translation="MPAIPRVPRLLLLGGAAALGMRALRQGRRIDFQDRVVLITGGSR
                     GLGLVLARTFAQEGARLAILARDAAEVRRAEDDLRAEGAEVRGFACDVRDQHQIDETI
                     QRIIDHYGRLDVLVNDAGVIQVGPLDHMTVADFKEAMGIHFWGPLYTMLAARPHLRKS
                     GQGRIVNIASIGGKIAVPHLLPYSASKFALVGLSDGMRAELARDGIRVTTVCPGLMRT
                     GSPPNAYFKGNNDAEYAWFTVSDSLPVTSVAAEHAARQIVDACRYGDPELTISIQAQL
                     VARANALFPGLTGEVMAVVNRLLPAPQPGAGSARKTGWQSQSPVAPSPLTRLSDEATT
                     ANNELDGHAPLTP"
BASE COUNT          711 a         1450 c         1483 g          587 t
ORIGIN      
        1 cgcccgctgt tcgcgttgta ggggccggtt gcaaaccggc ccggccgtca agaggagaac
       61 cccacatgca actggaaggg caagtcgcct tgatcaccgg cgccgggtcg ggcatcggca
      121 aggccgccgc gctgctgctg gcccgcgagg gcgcgaaaat cggtgtcctc agccgcgggg
      181 ccgaggaggt cgaagccgtc gccaaggaga tcgagcaagc gggcggcgcc gccctggccc
      241 tggtcgccga tatttcgcag cccggcgaga tgcagcaggc cgtcaagcaa ctggccgacc
      301 gctggggccg catcgacatc gtcttcgcca atgccggcat caacggcgtc tgggcgccgc
      361 tggaggaact cacccccgag gactgggacc acaccctgag catcaatctc aagggcacgt
      421 tcctgaccgt gaaatacgcc gtgccctacc tcaagaagca gggcggctcg gtgatcgtca
      481 cggcgtcggt caacggcacg cgcatcttca gcaacaccgg cgcgacggcc tactcctgct
      541 cgaaggccgg ccaggtggcc ttcaccaaga tgacggcgct cgaactggcc ccggacaagg
      601 tgcgcgttaa cgtcatctgc cccggcgcca tcaccaccca gatcgatgag aacaccgaga
      661 agcgcgacct ggaagatgta cgcattccgg tcaagtaccc ggaaggctcg cagccgctca
      721 ccggcggcaa ccccggcaca gcggagcagg tggcccaact ggtgctcttc ctggcctcgg
      781 acgcatccag tcacatcacc gggacagaag tctggatcga cggcgccgag tcgctgctgc
      841 gcggctaggc ggggcgggcc ggtttcaaac cggcccctac aacgcgagat tgcgcgggcc
      901 ggtttgaacc cggcccctac agcggcagat tgggcgggcc gagttgaaac cggcccctat
      961 acagcgcaga agcatggtgc cgggccgcga acggcagcgc aggtacagca gagggagaca
     1021 gccgtatggc agacatggaa tcgacacaac gcgacggcct gggcgacgtg aagcgctggg
     1081 tggccgtggc cgcgggcggg ggcctcacga tctacggcct gtcgcgccgt tcgctaggcg
     1141 gtgtcgcgct ggccgtggcg ggcggcggcc tgctggcgcg cggcctgctc ggcggccagg
     1201 ggggcgcgga atcggtggac gacgagtggg aaaccgggcg cgaacgccgg caggccgccg
     1261 cccaggaggc cggcgcggtg cacgtcgaaa aggcgatcac catcgagaaa tcgcccgccg
     1321 agctctacca gttctggcgc gatttcgaga acctgccgcg cttcatggac aacctggaat
     1381 ctgtgaccat ggcgggcgac gggcgctcgc actgggtcgc caaggcgccg ctcggcagcc
     1441 acgtcgagtg ggacgccgag gtgacggccg acgagccaaa cgagcgcatc gcctggtcgt
     1501 cggtggccga ggccgacgtg cagaatgcgg gcgaagtacg cttcgtgccc gcgcccggcg
     1561 accgcggcac ggaggtccac gtccgcctcg attacaaacc gccgggaggc aaggccgggg
     1621 ccatggtcgc caaactgttc ggcgacgaac ccggccagca ggtgggcgac gatctgcgcc
     1681 gcctcaagca gatgctcgaa gccggcgaag tcgccaccac cgcgggccag ccgtcgggcc
     1741 gcccgatgta ggggccggcc ccgctgatgg ccggccaggc taattgtgcg ttgtaggggc
     1801 cggtttgaaa ccggcccgcg ctcgccttcc gactttgctg gaggcggacg aatagggaga
     1861 ttgcgctgtg aaagctgtct gctggtgggc cgctaacgat gtccgcgtgg acaccgtgcc
     1921 cgaccccaag atcatcaacc cgcacgacgc catcgtgcag atcacctcga cggcgatctg
     1981 cggctcggat ctgcacctct acgacggcta catcccgacc atgctcaagg gcgatattct
     2041 gggccacgag ttcatgggcg agatcgtcga ggtggggtcc gaggtcgaga acctcaaggt
     2101 gggcgaccgg gtggtggtgc ccttccccat ctcctgcggc cagtgccact attgccgcaa
     2161 ggggctgtgg tcgctctgcg acaacaccaa ccccaaggcc gcgctggccg agggcatgta
     2221 cggctactcg cccgcgggca tctacggcta ttcgcacctg ctgggtggct atgccggggg
     2281 ccaggccgag tacgcgcggg tgcccttcgc cgatgtcggt ccgatcaaga tcccggcggg
     2341 gctgaccgac gagcaggtgc tgttcctgtc cgacatcttc cccaccgggt ggatggccgc
     2401 cgagaattgc ggcatccagc cgggcgacgt ggtcgcggta tggggttgcg gcccggtggg
     2461 ccagttcgcc gtcaagagcg cctacctgca aggcgccgag cgggtgatcg ccatcgaccg
     2521 catcccggag cggctgaagc tggccgagac ggcgggcggc gccgaggtga tcaactacgt
     2581 gggcaccgac gtggtcgagg agttgaagca gcgcaccggt ggccgcggcc ccgatgcctg
     2641 catcgacgcg gtgggcatgg aagcccacgg caccggcccg ctgtggtact acgaccgggt
     2701 ggcccaggcc acgcgcatgg agagcgaccg gcccctggtg atccgcgagg cgatccaggc
     2761 ctgcggcaag ggcggcaccg tctcgctggc cggtgtctac ggcggggtgg tcgataagat
     2821 ccccttcggc gcggccttca acaagggtct gaccctcaaa atgggccaga cccacgtgca
     2881 gcgctacctg caaccgctgc tcgaccgcat cgtggccggg gacatcgacc cgtcgttcgt
     2941 catcacgcac cggctggcgc tcgacgaggc gccggagggc tacaagatat tcaaggaaaa
     3001 ggaagacaac tgcatcaagg tggtgctcaa gccgtgaggc cggtcgcgcc gccggaaagg
     3061 agctagcgat gccggcaata ccgagagtac cgcgcctgtt gctgctgggg ggcgcggcgg
     3121 ccctgggcat gcgtgccctg cgccaggggc ggcgcatcga tttccaggac cgggtggtgc
     3181 tcatcaccgg cgggtcgcgc ggcctgggcc tggtgctggc gcgcacgttc gcccaggagg
     3241 gcgcgcggct ggcgatcctg gcccgcgacg ccgccgaggt gcggcgcgcc gaggacgacc
     3301 tgcgcgccga gggcgccgag gtgcgcggct tcgcctgcga tgtccgcgac cagcaccaga
     3361 tcgacgagac catccagcgc atcatcgacc actacgggcg gctcgacgtg ctggtcaacg
     3421 acgccggggt gatccaggtc ggcccgctcg accacatgac cgtggccgac ttcaaggagg
     3481 caatgggcat ccacttctgg gggccgctct acacgatgct ggccgcccgg ccccacctgc
     3541 gcaagagcgg ccagggccgc atcgtcaaca tcgcctccat cggcggcaag atcgccgtgc
     3601 cccacctgct gccctactcg gccagcaagt tcgcgctggt cggcctgtcc gacggcatgc
     3661 gcgccgaact ggcccgcgac ggcatccgcg tcaccacggt ctgccccggc ctgatgcgca
     3721 ccggttcgcc gcccaacgcc tacttcaagg gcaacaacga cgccgagtat gcctggttca
     3781 cggtcagcga ctcgttaccg gtcacgtccg tcgccgccga gcacgccgcc cggcagatcg
     3841 tggacgcctg ccgctacggc gatcccgaac tgaccatctc gatccaggcg caactcgtgg
     3901 cgcgcgccaa cgcgctcttc cccggcctga ccggcgaggt gatggccgtg gtcaaccgcc
     3961 tgctgcccgc gccgcaaccg ggcgcggggt cggcccgcaa gaccggctgg cagagccagt
     4021 cgcccgtggc cccctcgccg ctgacccgcc tctccgacga ggccaccacg gcgaacaacg
     4081 agctggacgg ccacgccccg ctcacgccgt aggcgacggg cctaaccccc ctaccccctt
     4141 cccggagcgg gaagggggga atcgccggtg ctaggaaacg tgactcccct ccccgcggcg
     4201 gggaggggtt gggggagagg ttggtcgggc g
//
LOCUS       DASUYC010000873         3042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_603699,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000873 DASUYC010000000
VERSION     DASUYC010000873.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603699"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VF276_14310"
     CDS             <1..475
                     /locus_tag="VF276_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019856594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6608075.1"
                     /translation="GAEIALGGHRPAGTAFERGFWYAPTVLTNVRNDMEIMQQEIFGP
                     ISPVMAFDDFEEAIALANDSKYGLSAYLFTNDLQRMMRAVQAIRFGEVYINRIGPEQF
                     QGFHTGYRLSGMGGDDGRHGYDHYFRKKTVYVNYGTPPTAGLMPYTTDETELPPQ"
     gene            483..2798
                     /locus_tag="VF276_14315"
     CDS             483..2798
                     /locus_tag="VF276_14315"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX6608076.1"
                     /translation="MAELPAALIAINPYEARTAAAIFERMFPADEAGPGATAIGVVHY
                     VDRALAGAYRDLAPAYRVGLATLDRAACTRFGQSFADCAPEQQDTLLGELEQGRLPDF
                     HVPPPREFFQTLRAHLQEGLFADPVYGGNRDKQGWRFLGHPGFWFENSAEENLAAEPA
                     LKGGVIQSLADVGYSLNGGPREPVEIPGYDPQRGGQPPAGPADVVLVGVGAAGALAAA
                     VLAGAGLRVVGLEVGPWRTNRDFVPDELASAYYCRGEMGLKFLSETPRWRLKADSPDR
                     EARFTLGRMMNGVGGSVIHWGGALRRLHPHHFQYRSYVRERWGEGALPEGHTLVDWPL
                     TYDDLEPYYTLIEDHIGVAGDGSRNPFVPRSKPYPLPPMRPFTMGETFRKATESLGLH
                     PYPTPVAVNSEPYNGFPATTYCAWSGGFGPFNNERWYPGLTWVPQALATGNFDLRTHC
                     RVVRVLTDAAGHAAGVEYVDANGHRQVQEARTVILCSYTFENVRLLLLSGDGRHPQGL
                     GNNAGQVGKHLMTKLWADVSGLVPDVIFNAHTGPAAQMWSLDDFISVDFDAPAHGFVG
                     GATPNIENQRLPIQISREALPPTVRSWGRPYKDHLRQWQHITAVRLQPDSLSYHANYL
                     DLDPHHRDRSGLGLPLLRITYDMQPNEHRLAEFMEGQAEAILRAMGATATWRGPRFNG
                     VVSSHDLGGCRMGEDPATSVVDPDLRVHDTPGLYVFSTAVFPTCHGVNPTLTMWAVCR
                     RAAERLAAQLRGAAPDAAPVSRATRSDRGAA"
     gene            complement(2761..>3042)
                     /locus_tag="VF276_14320"
     CDS             complement(2761..>3042)
                     /locus_tag="VF276_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608077.1"
                     /translation="KQIAARGGNPFRIEFEPYMDLLASEIGALPRLWRHPRLWRALLA
                     GPPLAARYRLDGPGRAASAAHILRSGNRPAAPATAAPATPLRDRSWSRG"
BASE COUNT          470 a         1030 c         1073 g          469 t
ORIGIN      
        1 gggggccgag atcgccctcg gcggtcaccg gcctgcgggc acggcgtttg agcgcggctt
       61 ctggtatgcg ccgaccgtgc tgaccaacgt gcggaatgat atggagatta tgcagcagga
      121 gattttcggc ccgatctcgc ccgtcatggc ttttgacgat tttgaagaag ccatcgcgct
      181 ggctaacgac agtaagtatg gcctgagcgc ctacctgttc accaacgacc tgcaacgcat
      241 gatgcgcgcc gtgcaggcca tccgcttcgg cgaggtctac atcaaccgca tcggaccgga
      301 gcagttccag ggcttccata ccggctaccg gctgagcggc atgggcggcg acgacgggcg
      361 gcacggctat gaccactact tccgcaagaa gacggtctac gtgaactacg gcacaccgcc
      421 caccgccggg ctgatgccgt ataccacgga cgagaccgag ttacccccac agtgaggagc
      481 ttatggctga actaccggcg gcgctgattg ccatcaatcc ctatgaagcg cgcacggcgg
      541 cggcgatctt cgagcggatg tttcccgcgg acgaggccgg gccgggcgcg acggccatcg
      601 gcgtcgtcca ctacgtggac cgggcgctgg ccggtgcgta ccgcgacctg gccccggcct
      661 accgggtggg gctggcgacc ctggaccgcg ccgcctgcac ccgcttcgga cagtccttcg
      721 ccgattgtgc gcccgagcag caagacacct tgctgggcga actcgaacag ggccgcttgc
      781 ccgactttca tgtgccgccg ccgcgcgagt tcttccagac gctgcgtgcc catctccagg
      841 aggggctctt cgccgacccg gtctacggcg gcaaccgcga caagcagggc tggcggttcc
      901 tgggccatcc cggtttctgg tttgagaact cggcggagga gaacctggcc gccgaaccgg
      961 cgctgaaagg cggcgtgatc cagtcgctgg ccgatgtggg ctactcgctg aacggcggcc
     1021 cgcgcgagcc ggtggagatc cccggctacg acccgcagcg cggcggccaa ccgcccgccg
     1081 gccccgccga tgtcgtgctg gtgggggtgg gcgcggcggg tgccctggcg gcggcggtcc
     1141 tggccggcgc cgggctgcgc gtagtcggcc tggaggtggg gccgtggcgc accaaccggg
     1201 acttcgtgcc cgacgaactg gcgtccgcct actattgtcg cggcgagatg ggcctcaagt
     1261 tcctgagcga gacgccgcgc tggcgcctca aggcggactc gccggaccgt gaagcgcgct
     1321 tcacgctggg ccgcatgatg aacggcgtgg gcgggtcggt catccactgg ggcggcgcgc
     1381 tgcggcgcct gcatccccac cacttccagt atcgctcgta tgtccgcgag cgctggggcg
     1441 agggagcact gccggagggc cacacgctgg tcgattggcc gctgacctac gacgacctgg
     1501 aaccgtacta cacgctgatc gaggaccata tcggcgtggc cggcgacggc agccgcaacc
     1561 cgttcgtgcc gcgcagcaag ccctatccgc tgccgccgat gcgccctttc accatgggcg
     1621 agaccttccg caaggcaacc gagtcgctgg ggctgcatcc ctatccgacg ccggtcgccg
     1681 tgaacagtga gccctacaat gggtttccgg ccaccaccta ttgtgcctgg agcggcgggt
     1741 tcggcccctt caataacgag cgctggtatc cggggctgac ctgggtgccg caagcgctgg
     1801 cgaccggcaa ctttgatctg cgcacccatt gtcgcgtcgt ccgcgtgctg actgatgccg
     1861 ccgggcatgc cgcgggcgtc gaatacgtgg acgccaacgg ccaccggcag gtgcaggagg
     1921 cgcgcacggt catcctgtgc agctacacgt ttgagaacgt gcggctgcta ctgctttccg
     1981 gcgatgggcg gcacccgcag gggctgggca acaacgccgg tcaggtcggc aagcacctga
     2041 tgacgaaact ctgggccgat gtgtcgggcc tggtgcccga tgtcatcttc aatgcccata
     2101 ccggtccggc ggcgcagatg tggagcctgg acgattttat ctcggtggac ttcgacgcgc
     2161 cggcgcacgg gtttgtcggc ggggccacgc ccaacatcga gaaccagcgc ctgccgatcc
     2221 agatcagccg cgaggcgctg ccgcccaccg tgcggtcgtg gggccggccg tacaaagacc
     2281 acctgcgcca gtggcagcac atcaccgcgg tacgtctgca acccgactcg ctctcctacc
     2341 acgcgaacta cctcgacctc gacccgcacc accgggaccg cagcggcctg ggcctgccgc
     2401 ttttgcggat cacctacgat atgcagccca acgagcaccg cctggccgag tttatggagg
     2461 gccaggccga ggcgatcctg cgggcgatgg gcgcgacggc gacctggcgc ggcccgcgct
     2521 tcaacggcgt ggtgagcagc catgacctgg gcggctgccg catgggcgag gaccccgcga
     2581 cctcggtggt cgatcccgat ctgcgggtgc atgacacgcc ggggctgtac gtgttcagca
     2641 cggcggtatt tccgacctgc cacggggtga acccgacgct gaccatgtgg gccgtgtgcc
     2701 ggcgcgcggc ggagcggctc gcggcgcagt tgcggggcgc cgcgccggac gccgcgccgg
     2761 tcagccgcgc gaccaggagc gatcgcggag cggcgtagcg ggagccgccg tcgccggtgc
     2821 ggcggggcgg ttgccggatc gcaggatgtg cgcggcggat gcagcgcggc ccggcccatc
     2881 gagccggtag cgggccgcga gcggggggcc ggccagcagc gctcgccaga gccgcggatg
     2941 gcgccacagc cggggcaggg cgccgatttc ggacgccagc aggtccatgt agggttcaaa
     3001 ctcgatgcgg aaggggttgc ccccgcgcgc agcaatctgc tt
//
LOCUS       DASUYC010000874         3856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_604010,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000874 DASUYC010000000
VERSION     DASUYC010000874.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604010"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VF276_14325"
     CDS             <1..541
                     /locus_tag="VF276_14325"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEX6608078.1"
                     /translation="QVASTVFRAAFNSGFDIIERHPHSYRVSWYEPPVGFDATVFSDE
                     GVDLRWRNNLPAPILMRTAIDNKAGTVTVSIYSAAQQPFTVSFDGPYTANRKPHGAAL
                     YENDPTLPAGKVVQVEHAKDGLDVTVYRVITDKATGKVLRKDKFFSRYVPWRDVYKRG
                     TGKSTTTNPEGSGVTVQKP"
     gene            699..1136
                     /locus_tag="VF276_14330"
     CDS             699..1136
                     /locus_tag="VF276_14330"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEX6608079.1"
                     /translation="MRSARRNLGAQLLMILVVLIGLGFGYSFAREVVQAQHLAGQAEA
                     ARQENERIAAENRKLQRDLQYYQSDAYAELRARTDLNMRRPDEQVILPVLPPGETTIT
                     APDSDMAAAPAAPAPASAVAPAPGPATGRNWDRWLALFAGTAR"
     gene            1206..1280
                     /locus_tag="VF276_14335"
     tRNA            1206..1280
                     /locus_tag="VF276_14335"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1238..1240,aa:Met,seq:cat)
     gene            1668..2426
                     /gene="rpsB"
                     /locus_tag="VF276_14340"
     CDS             1668..2426
                     /gene="rpsB"
                     /locus_tag="VF276_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX6608080.1"
                     /translation="MKGLLEAGVHFGHQTKRWNPKMRPYIFTERNGIHIIDLQQTVQG
                     LQQAMRFVADTVANGGKIIFVGTKKQAQDTVREEAERSGMFYVNKRWLGGMLTNFVTM
                     RARLRYLHDLEARRERGEFELLPKAESSKLNEEIAKLNETLGGIKDMRDLPAAVFIVD
                     PHRERIAITEAIKLGIPVIAMVDTNCDPDEVDYVIPSNDDAIRAVRIVTSKIADAANE
                     GLARRESARADAEVAQEMEYAGAVQGGVFEPDEE"
     gene            2585..3133
                     /gene="tsf"
                     /locus_tag="VF276_14345"
     CDS             2585..3133
                     /gene="tsf"
                     /locus_tag="VF276_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020373078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX6608081.1"
                     /translation="MEISSTTVKELREKTGAGVMDCKKALQEANGNFDRAVVILREKG
                     AMQLAKKLEREANQGIIDTYIHAGGRIGAMVELNCETDFVARTEDFRKLAHDIAMQVA
                     AMSPRYVRAEEAPDGAEGVEEQTLLKQAFIRDPKLTIEQLIHEAVGKLGENIQVRRFV
                     RFEVGAPVPGEAQGGGNGSTAG"
     gene            3309..>3856
                     /gene="pyrH"
                     /locus_tag="VF276_14350"
     CDS             3309..>3856
                     /gene="pyrH"
                     /locus_tag="VF276_14350"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003336945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX6608082.1"
                     /translation="MGTLPYGIDPLVVGSIAAQVRDVVAQGVQISIVVGGGNIWRGLE
                     ASSKGMDRSTGDYMGMLATVLNALALQDALEKIDVQTRVQSALTIQEVAEPYIRRRAL
                     RHMEKGRVVLLAGGTGNPYFTTDSAAALRALELGCQAILMAKNAVDGVYDADPRLDPA
                     ARRYFTLDYRHALEKNLRIMDST"
BASE COUNT          717 a         1262 c         1244 g          633 t
ORIGIN      
        1 ccaggtcgcc agcacggtct tccgcgcggc cttcaactcc gggttcgata tcatcgagcg
       61 ccacccgcac agctaccgcg tctcatggta cgagccgccg gtcggcttcg acgcgaccgt
      121 cttctcggac gagggtgtgg acctgcgctg gcgcaacaac ctgcccgcgc cgatcctcat
      181 gcgcaccgcc atcgacaaca aggccggcac ggtcactgtc tcgatctaca gcgcggccca
      241 gcagccgttt accgtgagct ttgacggccc ctatacggcc aaccgcaagc cgcacggcgc
      301 tgctctctat gagaacgacc cgacgctgcc cgcgggcaag gtcgtccagg tggagcacgc
      361 caaggatggc cttgacgtga ccgtctaccg cgttatcacc gacaaggcca ccggcaaagt
      421 gctgcgcaag gacaagttct tcagccgcta cgtgccgtgg cgcgatgtct acaagcgcgg
      481 cacgggcaag agcaccacga ccaaccccga agggtcgggc gtgacggtcc agaagccctg
      541 atcatcccga cgatacggcg ggcgcatctg cgggtgcgcc cgttcttttt gccctaaaat
      601 cggcccgcaa acctcttaac ataacacaca aggtgtgcta taatggggtc acatttcgcc
      661 ggtatccgtc gcccgcgtgg cttatccggg agctgcttat gcgttccgcc cgtcgcaacc
      721 tcggcgccca gttgctgatg atcctcgtgg tgctcatcgg cctgggtttt ggctacagct
      781 tcgcccgcga ggtggtccag gcgcaacacc tggccgggca ggccgaggcc gcccggcagg
      841 agaacgagcg catcgcggcg gagaaccgca agctgcaacg cgatctgcaa tattaccagt
      901 cggacgccta cgccgaactc cgcgcccgca ccgacctgaa catgcgccgc cccgacgagc
      961 aggtgattct gcccgtcctg ccccccggcg aaaccaccat caccgcgccg gacagcgata
     1021 tggccgccgc gcccgcggcc ccggcccccg ccagtgcggt cgccccggcg cccggccccg
     1081 ccacgggccg caactgggac cgctggttgg ccctcttcgc cggcaccgcc cgctgacatc
     1141 cgcccgaatc gatccgccaa agcttgacat cgcgcggcta atcgggtata atagaccaac
     1201 agcgacgcgg agtggagcag tggcagctcg ttgggctcat aacccaaagg tcgcaggttc
     1261 gaatcctgcc tccgctacca atgatagagg cccctgaaca gggggcctct tttttgtcgc
     1321 ccgcgccggt gggacatggg ccgcctgtag gcgatcgtgg tcgcccacag gttgcagcgg
     1381 tggccccatt tatgatatac ttactgttgg tgcgccgcgt gtgtgcgcgc cggatttgtt
     1441 ttattatgca cgcgcttcga ctgcgccggt cgtgcttcgc gttcgctgcg gcgaacgggg
     1501 gttccggcgc ggaatgacga gcgcggacgc agcctaacga tactaggagg ataagaataa
     1561 cattggttaa tgtcgtagag aatcccactg caacggcacc ggcccccgcc ccggtcgagc
     1621 gcgcgccccg cgaaacgcag acccgcgacg gcaattatgg catctcgatg aagggcctgc
     1681 tcgaagccgg tgtccatttt gggcatcaga ccaagcgctg gaaccccaag atgcgcccct
     1741 atattttcac cgagcgcaac gggatccaca ttatcgatct gcagcagacc gtgcagggct
     1801 tgcagcaggc gatgcgcttc gtggccgaca ccgtcgccaa cggcggcaag attatctttg
     1861 tcggcaccaa gaagcaggcg caggacaccg tgcgcgagga agccgagcgc tcgggcatgt
     1921 tctatgtcaa caagcgctgg ctgggcggca tgctcaccaa cttcgtgacc atgcgcgcgc
     1981 gcctgcgcta tctgcacgac ctggaagccc gccgcgagcg cggggagttc gagctgctgc
     2041 ccaaggccga gtcgagcaag ctgaacgagg agatcgcgaa gctgaacgag acgctgggcg
     2101 ggatcaagga tatgcgcgac ctgccggcgg ccgtcttcat cgtcgacccg caccgcgagc
     2161 gcatcgccat caccgaagcg atcaaactgg gcatcccggt catcgccatg gtggacacga
     2221 actgcgatcc cgacgaggtc gactacgtga tcccctcgaa cgacgacgcg atccgcgcgg
     2281 tgcgcatcgt cacgtcgaag atcgccgacg ccgccaacga ggggctggcc cgccgtgaga
     2341 gcgcgcgcgc cgatgccgaa gtggcgcagg agatggaata cgccggcgct gttcagggtg
     2401 gcgtgttcga gccggacgaa gaataaccaa tctcacagcc gccagagtca gccgtcgatc
     2461 tctgatcgct gacggtgccc tggcggctga ttactgtgcg ccggaccatc cggcggacag
     2521 ggcctgattg tcgagacatg cgccggggcg ggaagctacc ggcctgagta ggaggtatac
     2581 cactgtggag atttcgtcca cgactgtgaa agagctgcgc gagaagaccg gcgccggcgt
     2641 gatggattgc aagaaggccc tgcaagaggc caacggcaac ttcgaccgcg ccgtcgtgat
     2701 tctgcgcgag aagggcgcca tgcagcttgc caagaaactg gagcgcgagg cgaaccaggg
     2761 tatcatcgat acctatatcc atgccggggg ccgcatcggc gccatggtcg agttgaactg
     2821 cgagaccgac ttcgtggccc gcacggagga cttccgcaag ctggcccacg atatcgccat
     2881 gcaggtggcc gcgatgtcgc cgcgctatgt gcgcgccgag gaggcgcccg acggcgccga
     2941 gggcgtggag gagcagacgc tgctcaagca ggcgttcatc cgcgatccga agctgaccat
     3001 cgagcagttg atccacgagg cggtcggcaa gctcggcgag aacatccagg tgcgccggtt
     3061 tgtacgcttc gaggtgggcg cgcccgtgcc gggcgaggcc cagggcggcg gcaacggctc
     3121 caccgccggg taatcgagaa tggacggggc gggtctcatc ggacccgccc ttttgtatat
     3181 cacccccagg agggagcccg cttatgaccg attccattgc ccgcgcggag agcgccacct
     3241 gtgcctggcc ggcggagttc agccggccct atcgccgcgt gctgctcaag ttgagcgggg
     3301 aggcgctcat gggcacgctg ccctacggca tcgaccccct tgtcgtcggc agcatcgcgg
     3361 cccaggtgcg ggatgtggtg gcacagggcg tgcagatctc gattgtcgtc ggcggcggga
     3421 atatctggcg gggcctcgaa gcgtcctcca aaggcatgga ccgctccacc ggcgattaca
     3481 tgggcatgct ggcgaccgtc ctgaacgcgc tggccctgca agatgccctg gagaagatcg
     3541 acgtgcagac gcgcgtccag tcggccctta ccattcagga ggtcgctgaa ccctacatcc
     3601 ggcgccgcgc tttacgtcac atggagaagg ggcgcgtggt gttgctggcc ggcgggacgg
     3661 gcaaccccta tttcaccacg gacagtgccg cggccttgcg cgcgctggaa ctggggtgcc
     3721 aggccatctt aatggcgaag aatgccgtcg atggcgtgta tgacgccgat ccgcgactcg
     3781 atccggcggc gcggcgctat tttaccctgg actaccggca tgcgctggag aaaaacttgc
     3841 gcattatgga ctccac
//
LOCUS       DASUYC010000875         3101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1067638, whole genome shotgun sequence.
ACCESSION   DASUYC010000875 DASUYC010000000
VERSION     DASUYC010000875.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1067638"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VF276_14355"
     CDS             <1..612
                     /locus_tag="VF276_14355"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX6608083.1"
                     /translation="GGGGRKKGGPLAAIQGNGCLTYIALWVVGLVLLVSGVCAAGSGI
                     LSRIGLPIPGAGAATSSSGISISARPVAPAAAAPARAPAASQPVSQPAASTGGGNTTT
                     APSGVINSVPLQGAQGALITPYLAPAFYIVRPGDTLDTIAAMYGTTASSLRTYNRLTS
                     DQLQIGQVLYLPPATFNTVPNTGRNAGKDSDAPNDPTDANGNQ"
     gene            671..1072
                     /locus_tag="VF276_14360"
     CDS             671..1072
                     /locus_tag="VF276_14360"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEX6608084.1"
                     /translation="MLLLLVQSLISACSITVGPPPSTTPGPGEPTNTPIEQVHFDVTH
                     VPSPTALPPTSTPGPGGAVAPGATGKYTIKPGDTLSGIAQEFGITVEELVKLNNIVDP
                     NQIQAGQVLTVPAKGAPTSAPGPTASPTPGP"
     gene            complement(1423..2874)
                     /locus_tag="VF276_14365"
     CDS             complement(1423..2874)
                     /locus_tag="VF276_14365"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX6608085.1"
                     /translation="MVTIAALPKGSQPYTPEVEAYARGLIEAANTIRLPELFAAPHPD
                     ALYQLHDRNGVSVVAVRTPGLSEAQLIKLLRYRMAQYLVVHFVNHAQVYAGRIEHEPP
                     ALVAPGDVHVIAANSATGEILCYAVLRAAPQARPGCTMREQDRPLFPAEDLYGWGVFN
                     RLRIFPDLPVNKVREIGRFMKNQQLTALDELGARGPIEVGVAMFHILVGPLRMEVDAF
                     IGDIEEGVAGQNLAFFHTPMVVVHSTPPYESEDSYLFLRHQYRTIYPFAALVSDLTDP
                     MRTRLPQIEQALALPGKQGVLALVGLKRDLQATRSSLAPPDYLDPLIDSALPQQGIPM
                     QQRRAMLDVGERVRQFPLFSGLSVAEATVLGSFMECIEVPAGEVIVRRWECGDSLYLV
                     ESGEAIVQIARTNGPPVQLAAINAGDYFGEIALLTGGERTADVVACTPMMLLKLGRDA
                     YVRYLRHVAEVDQNLAHTATRRATAVLRSVSAA"
BASE COUNT          570 a         1037 c          978 g          516 t
ORIGIN      
        1 ggtggcggcg gcaggaaaaa gggcggtccc ctggcggcaa tccagggcaa tggatgtctg
       61 acctatatcg ccctctgggt ggtcggcctg gtgttgctgg tcagcggggt gtgtgccgcc
      121 ggttccggca tcctatcgcg gatcggcctg ccaatccccg gcgccggtgc cgccaccagc
      181 tcatccggca tcagcatcag cgccaggccg gtggcccccg cggcagcggc tcccgcccgc
      241 gccccggcgg catcccagcc ggtcagccag cccgcggcgt ccacgggcgg cggcaatacg
      301 accacggcgc ccagcggcgt catcaactcg gtgcccttgc agggcgcgca gggggccttg
      361 atcaccccat acctggcccc ggctttctac atcgtgcgtc cgggcgatac gctcgacacg
      421 attgcggcaa tgtacggcac gacagcgtct agtttacgca cctataaccg gctcactagt
      481 gaccagttgc agatcggaca ggtgctctat ctgccccccg ccaccttcaa tacggtgccg
      541 aatacggggc gcaatgccgg taaggatagc gatgctccga acgacccaac cgacgcaaac
      601 ggcaaccagt aatccatcca tccgcccggt cgcacgacat atactgactc ccgcaacccg
      661 gcggatcgcc gtgctcttac tgctggtgca aagcctgatc agcgcttgca gtatcaccgt
      721 cggcccgccg cccagcacca cgccgggacc gggcgagccg accaatacgc ccattgagca
      781 ggtccacttc gacgtgaccc atgtgccgtc gcccactgcc ctgccgccga ccagcacgcc
      841 cggccccggc ggcgcggtcg cgcccggcgc cacgggcaaa tatacgatca agcccggcga
      901 taccctctcc ggcatcgccc aggagttcgg catcaccgtg gaagaactgg tcaaactgaa
      961 caacatcgtg gacccgaacc agatccaggc cggccaggtg ctcacggtcc cggcgaaagg
     1021 cgcccccacc tcggcccctg gaccgacggc gtctccgact cctggtcctt gacagatcat
     1081 ttggtagtag ttataataag ctcagttgta agcaatgcac ctccccctgg gaggtttccg
     1141 cagaggaata aggaggtgat gcccatggat agtagtaagc atggcatcac cagcaaggtc
     1201 agctagatct agcgcaagcg aaatccagcg tgctggtgat gcaaacgtaa aaaggacccc
     1261 gtccccctaa aggacggggt tccttttttc ccccggaata acgtatcctc cgccccgccc
     1321 caaccccatt gcccgattat tcgtctccat ctctgaaagc cggcgaagcc cgaccagccg
     1381 gccgggcctc acctttaatg tggtttgtaa cagcgggttg gatcaggcgg cggacacgct
     1441 gcggaggacc gcggtggccc gccgggtggc ggtgtgggcg aggttctgat cgacttccgc
     1501 gacatggcgc aggtagcgca cataggcgtc ccggccgagt ttcagcaaca tcatgggcgt
     1561 gcaggccacc acgtcggcgg tgcgttcgcc gccggtcagc agcgcgattt cgccgaaata
     1621 atcgcccgcg ttgatggcag ccagctgcac gggcgggccg ttggtccggg caatctggac
     1681 gatcgcttcg cccgattcca ccagatagag gctgtcgccg cactcccagc ggcgcacgat
     1741 cacctcgccc gccggcacct cgatgcattc catgaacgac cccagcacgg tggcctcggc
     1801 cacggacagc ccgctgaaca gcgggaactg gcgcacgcgt tcgcccacgt ccagcatggc
     1861 ccggcgttgc tgcatcggga tgccttgctg gggcagagcc gaatcgatca gcgggtccag
     1921 gtagtcgggc ggcgccaggc tgctgcgggt cgcctggagg tcacgtttga ggccgaccag
     1981 ggccagcacg ccttgcttgc ccggcagcgc cagcgcctgc tcgatctgcg gcagccgcgt
     2041 gcgcatggga tccgtcagat ccgagaccag agccgcgaac gggtaaatgg tgcggtactg
     2101 gtggcgcagg aacaggtacg agtcctcgct ctcgtagggc ggggtgctgt gcacgacgac
     2161 catgggcgtg tggaaaaagg ccaggttttg ccccgcgacg ccctcttcga tgtcgccgat
     2221 aaaggcatcg acttccatgc gcagcgggcc gaccaggatg tggaacatcg ccacgcccac
     2281 ttcgatcggg ccgcgcgccc ctagttcgtc cagcgccgtc aactgctgat tcttcatgaa
     2341 gcgcccgatc tcgcgcactt tgttgacggg cagatcggga aaaatccgca ggcggttgaa
     2401 gacgccccag ccgtagaggt cctcggcggg aaagagcggc cggtcctgct cgcgcatcgt
     2461 gcagccgggg cgggcttgcg gcgcggcgcg cagcaccgca tagcagagga tctcgccggt
     2521 ggcgctgttc gcggcaatga cgtgcacatc gcccggcgcg accagcgccg gcggctcatg
     2581 ctcaatgcgc ccggcataga cctgggcatg gttgacgaaa tggaccacca ggtactgggc
     2641 catgcgatag cgcaggagtt tgatcaattg ggcttcactc aggcccggcg tgcggacggc
     2701 caccacgctg acgccgttgc ggtcgtgcag ttgatagagg gcatccggat gcggcgccgc
     2761 aaacaactca ggcagccgga tcgtgttcgc cgcctcgatc agcccgcggg cgtatgcttc
     2821 gacttcgggg gtatagggtt gggaaccttt gggcagggct gcaatcgtaa ccatcgtttc
     2881 tccctcgtgc agtgtcatgg ccgcgacgct actgtgttac ctctattaga ccacagaatc
     2941 cgcgaatccg atcccaccaa aaacccgaca aaaacccgac aaggacttgc cgcccgccca
     3001 ctgggcgggt agccatggcg ccgcgcaggg atgcgcggcg tggcacgagg aaacgttgcg
     3061 tactgggagg aagccaggac cgcctgggtt ttgtcattct g
//
LOCUS       DASUYC010000876         3999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_604445,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000876 DASUYC010000000
VERSION     DASUYC010000876.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604445"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            478..1590
                     /gene="carA"
                     /locus_tag="VF276_14370"
     CDS             478..1590
                     /gene="carA"
                     /locus_tag="VF276_14370"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEX6608086.1"
                     /translation="MTEKMAGLLVMADGTTIRAGGAGAPGIAVGELVFQTSMVGYQEA
                     LTDPSYCGQLLIFTYPLVGNYGTGAAMDQSARIRAHGMIASDLMDSAGHRHTGGDLDA
                     LLRVAEVPALIGADTRFLTRRVRTEGVIAAALAVASPDRLPAVADLQAQAAAFDYDAI
                     DFVAGCSTPAPVWYPPARPEAPRIVLVDYGTKTSTLEHLRASGAGVWVVPARSTAAEI
                     LALRPDGVLLSNGPGDPSALGYAVANVRDLLDAARVPIFGICLGHQLLTLAAGGRTIK
                     MRFGHRGVNQPVLELDTGRVAVTTQNHGYVVDGEAIPPGFRVTHTNLNDGSVEGIAHL
                     TRPIWGVQWHPEAHPGPTDTRHLLDRFLAATEAIYA"
     gene            1583..>3999
                     /gene="carB"
                     /locus_tag="VF276_14375"
     CDS             1583..>3999
                     /gene="carB"
                     /locus_tag="VF276_14375"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009455.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX6608087.1"
                     /translation="MPKDPSLHKVLIIGSGPIVIGQAAEFDYAGTQACLACREEGVQV
                     VLVNSNPATIQTDPGTADKVYIEPLTVESLTGIIRRERPDGLIATMGGQTGLNLSVAL
                     EDAGVLAEYGVRVLGTGIPTIRQAEDREAFADLMIAAGQPVLPHTTVNSLAEAEAFAA
                     EYGYPLVVRAPFCLGGTGSGHAADRAELAALVERGLEASPSGNLLVEKSVAGWSEIEY
                     EVLRDAADNAIIVCNMENLDPMGIHTGESIVVAPSQTLSDRDYHYMRTTALNIVRALD
                     VRGGCNCQFAWNPATGELAVIEVNPRLSRSSALASKAAGYPIARVAAKIALGYTLAEL
                     RNDITSASSSAEPALDYVVVKLPRWPFDKFRHVDQHLGMTMKSTGEVMAIGRTFEAAL
                     LKALRSLDTRTAWLEVSPAWTPERIRHALTHPTPERLAAVYNALAQGWTAEDIVNLTK
                     IHPWFIARLAAIYDMEAQVAAGLTPELLTAAKRMGFADEHLAQLAGDGRDIRAERLAL
                     GIRPVYKMVDSCAGEFAASTPYFYSTYAQDDEATPLPGPKAIVLGSGPIRIGQGIEFD
                     YSSVHAVQTLRAAGIGAIVINNNPETVSTDYSLSDRLYFEPLTLEEVLNVIDQEREGL
                     LGVIAQFGGQTALNLIAPLAAAGVPILGTTPDAIAATEDRRQMGDLCAELGIPTPAWT
                     IAQGEDEVVAGAAGIGFPVLVRPSYVLGGRGMRIVHSAADLTRYLAELGPHLRRRPVL
                     IDRFLQDAVEVDVDGVADGHEVYTVVMEQLEAAGVHSGDSTCVYPPQTLSPAVVAQVQ
                     TYTRRLV"
BASE COUNT          678 a         1434 c         1307 g          580 t
ORIGIN      
        1 aaacaggact aattctaatc ctattcttcc cgcccttcac gccttcgcgg tgacaaaaaa
       61 ccgggtgggg cttgacacgc gccccccgga tgtgctaaac tgcatggagc caagtgaata
      121 gcgaccaact gccgtatgcc cgcgatgatg tggaatagta gtgacaattg aagtttgcag
      181 cgaattggga cctggtgtga gcccgagaag cttcactgtc gcgaacgcgt cacagagcgg
      241 tccgccgaag aaagtccgat ggcgtgcaag taggagggag tgggtggttc ccacgcgagc
      301 aataaccgag agcggtcaca ggccactaaa tgtgacaatt agggtggtac cacgggagat
      361 tcgactctcg tcccttttgc gggacgggag ttttttattg cccaaaaacc gggtggttgc
      421 gtgacggagt gaggtatacc cgaggtacca cacgcgtgac acaaggagac tttgcacatg
      481 actgagaaga tggccggcct gctggtgatg gccgacggca cgacgatccg cgccgggggc
      541 gcgggcgccc cagggatcgc ggtgggcgaa ctggtcttcc agaccagcat ggtcggctat
      601 caggaagcgc tgactgaccc gtcctattgc ggccaactgc tgatctttac ctacccactg
      661 gtgggcaact acggcaccgg cgccgcaatg gaccagagcg cgcgcatccg cgcgcacggc
      721 atgatcgcca gcgacctgat ggacagcgcg ggccaccgcc acaccggtgg cgacctcgac
      781 gcgctgctgc gcgtcgccga ggtgcccgcg ctgatcggcg ccgacacgcg attcctgacc
      841 cgccgcgtgc ggacggaggg cgtcatcgcc gccgcgctgg ccgtggcctc gcccgaccgg
      901 ttgcccgccg tcgccgacct gcaggcgcaa gcggccgcct tcgactacga cgcgatcgat
      961 ttcgtggccg ggtgctcaac gcccgcgccg gtctggtatc ccccggcgcg acccgaggcc
     1021 ccgcgcattg tgctggtgga ttacggcacc aagaccagca cgctggagca cctgcgcgcg
     1081 agcggcgcgg gcgtctgggt ggtcccggcg cgcagcaccg ccgccgagat ccttgccctg
     1141 cggcccgacg gcgtgctgct ctccaacggc cccggcgacc cgtcggcgct cggctacgcg
     1201 gtcgccaatg tgcgcgacct gctggacgcg gcgcgcgtgc cgatcttcgg catctgtctg
     1261 ggccaccagt tgctgaccct ggccgcgggc gggcgcacga tcaagatgcg cttcggccat
     1321 cgcggcgtga accagccggt gctcgaactc gacaccgggc gcgtcgccgt caccacgcag
     1381 aaccacggct acgtcgtgga cggcgaggcg atcccgcccg gcttccgggt gacgcacacc
     1441 aacctgaacg acggctcggt ggagggcatc gcccacctga cgcggcccat ctggggcgtg
     1501 cagtggcacc ccgaagccca ccccggcccc accgataccc gccatctgct cgaccggttc
     1561 cttgccgcca cggaggccat ctatgcctaa agatccatcc ctgcacaaag tgctgattat
     1621 cggctccggc cccatcgtca tcggccaggc cgccgagttc gactatgccg gcacccaggc
     1681 ctgcctcgcc tgccgcgaag agggcgtgca agtggtcctg gtcaactcca acccggcgac
     1741 gatccagacc gaccccggca ccgccgacaa ggtctacatt gagccgctga cggtcgagag
     1801 cctgaccggc atcatccgcc gcgagcggcc cgacggcctg atcgccacaa tgggcgggca
     1861 aaccggcctg aacctgtccg tggcgctgga ggacgcgggc gtgctggccg agtacggcgt
     1921 gcgcgtgctg ggcacaggca tcccgaccat ccgccaggcc gaggaccgcg aagccttcgc
     1981 cgacctgatg atcgctgcgg gccagccggt gctgccgcac accaccgtca acagcctggc
     2041 cgaggccgag gcgttcgccg ccgaatacgg ctacccgctg gtggtgcgcg cgcccttctg
     2101 cctgggcggc accgggtccg gccacgccgc cgatcgcgcc gaactggccg cgttggtcga
     2161 gcggggcctg gaggccagcc ccagcggcaa cctgcttgtg gaaaagagcg tcgccgggtg
     2221 gtcggagatc gagtacgagg tgctgcgcga cgcggcggac aacgccatca tcgtctgcaa
     2281 catggagaac ctcgacccga tgggcatcca taccggcgag tccatcgtcg tggcgcccag
     2341 ccagacgctg tccgacagag actaccacta catgcgaacg acggcgctca acatcgtacg
     2401 cgcgctcgac gtgcgcggcg gctgcaactg ccagttcgcc tggaacccgg ccaccggcga
     2461 actcgccgtg atcgaggtca acccgcgcct ctcgcgctcg tcggcgctgg cctccaaggc
     2521 cgcgggctac cccattgccc gcgtggccgc caagatcgcc ctcggctaca cgctggccga
     2581 attgcgcaac gacatcacca gcgccagcag cagcgccgaa cccgcgctcg actacgtggt
     2641 ggtcaagctg ccgcgctggc ccttcgacaa gttccgccac gtggatcagc acctgggcat
     2701 gaccatgaaa agcaccggcg aggtgatggc gattggccgc accttcgagg cggcgctgct
     2761 caaggcgctg cgcagcctcg acacccgcac cgcctggctg gaagtcagcc cggcctggac
     2821 gccggagcgc atccgccacg ccctcaccca ccccacgccc gaacggttgg ccgccgtcta
     2881 caacgcgctg gcccagggct ggaccgctga agacatcgtc aacctaacca agatccaccc
     2941 ctggttcatc gcccggctgg ccgcgatcta cgacatggaa gcccaggtcg ctgccggact
     3001 gacgcccgaa ttgctcacgg ccgccaagcg catgggcttc gccgacgagc atctggcgca
     3061 actggcgggc gacgggcgcg acatccgcgc cgaacggctg gcgctgggga tccgcccggt
     3121 ctacaagatg gtcgatagct gcgccggcga gttcgcggcc tccacgccct acttctactc
     3181 cacctacgcg caggacgacg aggccacgcc gctgcccggc cccaaggcca tcgtcctggg
     3241 cagcggcccc atccgcatcg gccagggcat cgagttcgac tactcgtcgg tccacgccgt
     3301 gcagacgctg cgcgccgcgg gcatcggcgc catcgtcatt aacaacaacc ccgagacggt
     3361 cagcaccgat tactccctga gcgaccggct ctacttcgag ccgctgacgc tggaagaggt
     3421 cctgaacgtg atcgaccagg agcgcgaggg cctgctgggc gtgatcgccc agttcggcgg
     3481 gcagacggcg ctcaacctga tcgccccgct ggccgcggcg ggcgtgccga tcctgggcac
     3541 cacgcccgac gccatcgccg ctaccgagga ccggcgccag atgggcgacc tctgcgcgga
     3601 actgggcatc cccaccccgg cgtggaccat cgctcagggg gaggacgagg tggtggcggg
     3661 cgcggccggg atcggcttcc cggtgctggt gcgcccgtcc tacgtgctcg gcgggcgcgg
     3721 gatgcgcatc gtgcacagcg ccgccgacct cacccgctac ctggccgagc tggggccgca
     3781 tctgcgccgg cgcccggtgc tcatcgaccg cttcctgcaa gacgccgtcg aagtggacgt
     3841 ggacggcgtg gccgacgggc acgaggtcta caccgtggtc atggagcaac tcgaagcggc
     3901 gggcgtccac tccggcgaca gcacctgcgt ctatccgccg caaaccctca gcccggcggt
     3961 ggtcgcccag gtgcagacct acacccgccg cctggtcga
//
LOCUS       DASUYC010000877         3418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_604642,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000877 DASUYC010000000
VERSION     DASUYC010000877.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604642"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /locus_tag="VF276_14380"
     CDS             complement(<1..955)
                     /locus_tag="VF276_14380"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inactive serine/threonine-protein kinase VRK3"
                     /protein_id="HEX6608088.1"
                     /translation="MATTQPGTAVPQVCPTCHETVIASDGFCPSCGSPMNLSAPPAAP
                     GRAPAPGSAPAASASPGACPVCGAPLRPGAEFCPNCGASVADAVRSSQLAAAPVGSPV
                     LPSTPSGVPAPATCPKCGAPQRPGAKFCKECGYRFNEIATAIPSTSGGLTQLKVGQVL
                     AGRYKIVKVVGGGGMGAVFQAVDTHLQTREEPEGRLCAVKAILNTDDPELLAAAVEER
                     EMLIRLDHPNIVRIYDIVNEGGVPYIVMAFVKGTSWKQMYDNNGGPLPESEALRLLLG
                     IRGAFEYLHHRTPPVVYRDFKPGNVIQVDEPGGGTRQVLIDL"
     gene            1224..2534
                     /locus_tag="VF276_14385"
     CDS             1224..2534
                     /locus_tag="VF276_14385"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX6608089.1"
                     /translation="MIRRRSFAANAPEIVGPLLAAGLLGVLLLVLLSPRLGFALPTDP
                     NVVRIYSSVPMESFDSIAHGVQMAVDEAGGKAGNFRVEYVPLTDDKRQSDGSYHWDAD
                     VELANARRAAADPDAMVYIGTYNSGAAKISIPVLNRVRLTMISPGNTYPGLTKPGTGT
                     AGEPWIYYPLGVQNYFRVVPADDLQGNAGAAYAQQIGAKSVYVIDDTELYGHGIAVIF
                     AQAAKDLGLQVVGGPESIDTHTDDYAALASKIVAAAPDLVYFGGVTANHPARVLRDLR
                     RAGFQGTFMGADGIVGSDFLNDYKSSGGSGSPGSVYATLVGLPANKLTGAGQDWYQRY
                     ISRYPNDRNPDFAPFGYEAGRVAVHAIATAGVKDREAIRQATAATKSTDLGPNILGGA
                     WQFDANGDTSLVTISVQQLGADWAYQGVMSYDPQAKTWSFRQGQ"
     gene            complement(2560..3351)
                     /locus_tag="VF276_14390"
     CDS             complement(2560..3351)
                     /locus_tag="VF276_14390"
                     /inference="COORDINATES: protein motif:HMM:NF024628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEX6608090.1"
                     /translation="MCRLFGLIAPRPVKITFPLLDAAESLRRQSLLNPDGWGVGYYAG
                     GHAVVRKQPEAAHGSPGFSRVAEHARSHLFVAHVRLRTAGGVNLANTHPFSTGGWILA
                     QNGGIGGAWHKRIKAEVGWGRIAGKTSGEHLLYWLYARAGHLRGAARDAAIVAAVREL
                     VADPVSIGSANFVLATGDQLYAFRFSPPQGSARSLFYLRRVPPSAHATPGTEASPGAG
                     IPRTAAVLVASEQVTGPDEPWQPVANGCLLIARRDLSLDLERIAA"
BASE COUNT          529 a         1201 c         1179 g          509 t
ORIGIN      
        1 ccaggtcgat cagcacctgg cgcgtgccgc cgcccggctc gtccacctgg atcacattgc
       61 ccggcttgaa gtcgcggtag acgaccggcg gggtgcggtg gtgcaggtac tcgaaggcgc
      121 cgcggatgcc gagcagcagg cgcaacgcct cgctttcggg cagcgggccg ccgttgttgt
      181 cgtacatctg cttccaactg gtgcccttga cgaaggccat gacgatatag ggcacgccgc
      241 cctcgttcac aatgtcgtag atgcgcacaa tgttggggtg atccaggcgg atcagcatct
      301 cgcgctcttc gacggcggcg gcgagcagtt cgggatcgtc ggtgttcagg atggccttga
      361 cggcgcaaag gcggccctcg ggctcctcgc gcgtctgcaa gtgcgtatcg acggcctgga
      421 acacggcgcc catgccgccg ccgccgacga ccttgacgat cttgtagcgc ccggccagca
      481 cctggcccac cttgagctgg gtcagacccc cgctggtgct ggggatggcc gtggcgatct
      541 cgttgaagcg gtagccgcac tccttgcaga acttggcgcc ggggcgctgc ggcgcgccgc
      601 acttggggca ggtggccggg gcgggcacgc ccgacggggt gctcggcagc accggcgacc
      661 ccaccggcgc ggcggcaagc tggctgctac gcacggcatc ggcgaccgag gcgccgcaat
      721 tggggcagaa ctcggcgccg gggcgcaggg gcgcgccgca caccgggcac gcccccggcg
      781 aggcgctcgc cgcgggggcg ctgcccggcg cgggcgcgcg ccccggtgcc gccggggggg
      841 cgctcaggtt catgggcgag ccgcatgagg ggcaaaagcc atccgaggcg ataacggttt
      901 cgtggcaggt ggggcaaacc tggggcaccg ccgtgcccgg ttgcgttgta gccaaatccc
      961 tggactccct ctttcgcgcg cgtgatctga tcgtggtatg tcggtcccgg ctcgcgcttg
     1021 caccgggcgc gtccggcggc tatactgggg cgtaaccacc ggcactggtc ttagtatact
     1081 cagattcggc gttagtgtat agtcccgacc gggccggctg tcctgcgtaa cgtcgtcatg
     1141 ggaatctacg taatcgcagt agcgaaaggt tacaccgccc gcttgtttgc ataccggtct
     1201 ctgttccggg aggaaggtcc atcgtgataa gacgtcgttc gtttgccgcc aatgcgccgg
     1261 agatcgtcgg cccgctgctg gccgccgggc tgctcggcgt gctgctgctg gtgttgctca
     1321 gcccgcgcct gggcttcgcc ctgcccaccg accccaatgt cgtgcgcatc tattcgagcg
     1381 tgcccatgga gagcttcgac agcatcgccc acggcgtgca gatggccgtg gacgaggccg
     1441 gcggcaaggc cggcaacttc cgcgtcgagt atgtgccgct gaccgacgac aagcggcaga
     1501 gcgacggcag ctatcactgg gacgccgacg tggaactggc gaacgcgcgt cgcgccgccg
     1561 ccgaccccga tgccatggtc tacatcggca cctacaacag cggcgcggcc aagatcagca
     1621 tcccggtgct caatcgcgtc cgcctgacga tgatcagccc cggcaacacc taccccggcc
     1681 tgaccaagcc cggcaccggc acggcggggg aaccctggat ctactacccg ctgggcgtgc
     1741 agaactattt ccgcgtggtg cccgccgacg acctgcaggg caacgccggg gccgcctacg
     1801 cgcagcagat cggcgccaag tcggtctacg tgatcgacga caccgaactc tacggccacg
     1861 gcatcgccgt catctttgcc caggcggcga aggatctcgg cttgcaggtg gtcggcgggc
     1921 cggagagcat cgacacgcac accgacgact atgccgcgct ggcgagcaag atcgtcgccg
     1981 ccgcgcccga cctggtctac ttcggcggcg tcacggcgaa ccacccggcg cgcgtgctgc
     2041 gcgacctgcg ccgcgccggc ttccagggca cgttcatggg cgcggacggc atcgtcggct
     2101 cggacttcct caacgactac aagagcagcg gcggcagtgg cagccccggc tcggtctatg
     2161 ccaccctggt cggcctgccc gccaacaagc tgaccggcgc cgggcaggac tggtaccaac
     2221 gttatatcag ccgctacccc aacgaccgca accccgactt cgcgcccttc ggctatgaag
     2281 cgggccgcgt cgccgtgcac gccatcgcca cagccggggt caaggaccgc gaggcgatcc
     2341 gccaggccac cgccgccacc aagtccaccg acctggggcc gaacatcctg ggcggcgcct
     2401 ggcagttcga tgccaacggc gacacgtcgc tggtcacgat cagcgtgcag caactcggcg
     2461 ccgactgggc ctaccagggc gtgatgtcct acgacccgca ggccaagacc tggtcgttcc
     2521 gccaggggca ataaccagcc gggcgcaggc ccgcaggcgt tacgcggcga tgcgttcgag
     2581 atccaggctg aggtcgcggc gcgcgatcag caggcagccg ttcgccaccg gctgccaggg
     2641 ttcgtccggc cccgtgacct gctcggaggc gaccagcacc gccgcggtgc gggggatgcc
     2701 ggcgccgggc gaggcctccg tccccggcgt ggcgtgcgcc gagggcggca cgcggcgcag
     2761 atagaacagc gagcgcgcgc tgccctgcgg cggcgagaag cggaacgcgt ataactgatc
     2821 gcctgtggca agcacgaagt tggcgctgcc gatggacacc gggtccgcga ccagttcccg
     2881 cacggcggcc acaatcgccg cgtcgcgcgc cgccccgcgc aggtgcccgg cgcgggcgta
     2941 cagccagtac agcaaatgct ccccgctcgt cttgccggcg atgcgccccc acccgacctc
     3001 ggccttgatg cgcttgtgcc acgcgccgcc gatcccgccg ttttgcgcca ggatccagcc
     3061 gccggtgctg aagggatggg tgttggcgag attcacgccg cccgccgtgc gcagccgcac
     3121 atgggcgaca aacaggtgcg agcgcgcgtg ttccgcgacg cggctgaagc cgggcgagcc
     3181 gtgcgccgcc tccggctgct tgcgcaccac ggcgtgcccg cccgcgtagt agcccacccc
     3241 ccagccatcg gggttgagca ggctctgccg gcgtagcgac tccgccgcat ccagcagcgg
     3301 gaaggtgatc ttgaccgggc ggggcgcaat caggccaaag aggcggcaca taccagatgc
     3361 tttcgttttg tccgcggggt ccgcaaccgg gcgggttaca gctcccacag tgattccg
//
LOCUS       DASUYC010000878         3057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1229503, whole genome shotgun sequence.
ACCESSION   DASUYC010000878 DASUYC010000000
VERSION     DASUYC010000878.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229503"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VF276_14395"
     CDS             complement(<1..238)
                     /locus_tag="VF276_14395"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6608091.1"
                     /translation="MITLLLVDDDAAIRRTLGIRLGLEPDLVIAGEADTGAAALALAE
                     SLQPAVVLMDVQMVGMDGITTAELIRARVPATRVV"
     gene            542..2200
                     /locus_tag="VF276_14400"
     CDS             542..2200
                     /locus_tag="VF276_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608092.1"
                     /translation="MRQYAITARARTRMAGLLLLGAVLGSCSGTAPVPTPVAGSTAPP
                     PGLPATSTAPPLSGATATPSAPAATPTPVPPAATPTSTATISAARTVFYQVFRPTTVP
                     DWLTPASELMRDETMPSRLVQHYDAGSTRDALVWMEGPILPGRAEPDGQSVVVGARQG
                     RYSEQNGVRRLVLAVDGTEIELDGQANVTREVLIEVAASAQSVPMRVAVRLDATDAPV
                     LPPAQPALFPLSFADPLHGWALASCPGNAACAPEIRATVDGGETWQPAGPAPANASQL
                     RFTTPRDGWAFDPGFWATHDGGRTWTANPVLPAVSALEVAGDTVWAIAATPTGTVAPA
                     ATLYVSSDSGATWQTVSHPPITGPAWQLVRAGPDAGWILSWGNYGYPRQGPIGTISAT
                     QDAGRTWQPLPDPCVGVGGVEDRLAAADAHELWLLCGALPASGAAEEKALFQSTDSGG
                     HWTLNAAWESGRHELPAGQVVDLAVPVPGHPWIVAMRPGSVYRPTAAGQPWIPAIPSD
                     LASPGDRSVGSVQFIDAQHGWFSTAGDLFRTQDGGQTWDMLPHP"
     gene            complement(2412..2900)
                     /locus_tag="VF276_14405"
     CDS             complement(2412..2900)
                     /locus_tag="VF276_14405"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX6608093.1"
                     /translation="MKLRILIGLLLAVGLLAGCGEAAPTATPVPPTPAPAARGGGANG
                     PEHITVQHILIGFQGSVPGKPITRTQEQAKTLAYQLLDKAKSGADFDQLVRDNTDDSP
                     PGIYSMSNLGVPPADGEYPREGMVPAFGNVGFTLQVGQIGIADFDPQTSPFGWHIIKR
                     LK"
BASE COUNT          451 a         1041 c         1101 g          464 t
ORIGIN      
        1 tgaccacgcg cgtggctggc acccgcgccc ggatcagctc ggcggtggtg atgccgtcca
       61 tgcccaccat ctgcacatcc atcaacacga cggcgggctg gagcgactcg gccagcgcga
      121 gggccgccgc gccggtatcg gcctccccgg cgatcaccag gtcgggttcg agcccgagcc
      181 ggatgccgag tgtgcggcgg atggctgcgt cgtcgtccac cagtagcagt gtaatcatgg
      241 ccccgccggt cccctgtctc cttgcgcccc gtcgcgtgcc gggcagcgag gtacgtgtcg
      301 ccgcgccggc aacctactac cttccagtgt agcttaccgg ccggcaaggc gcgacgaccc
      361 gtggatacaa accggaggga acccttgggc ggagggcatc tctatcgaaa gatgcaccgc
      421 gtttcgtccg tctgcccctg gacctgcgcc cttgccggtg cggcgggaaa gggctagaat
      481 cgagagggag acatcaataa aggtggcgca ggcggcccgc tcaccggcaa ggggcagatc
      541 gatgagacaa tacgcgataa cggcgcgggc gcgcacccgc atggcggggt tgttgctgct
      601 gggggcggta ttggggagtt gtagcgggac ggccccggtg cccacgccgg tggccgggag
      661 cacggcgccg ccccccggcc tgccggccac ctccacggca cccccgttgt ccggcgcgac
      721 cgccacgcca tccgcgcccg cagcgacccc gacgccggtc ccgcccgcgg caacccctac
      781 gtcaacagcg actatctccg ctgcgcgcac cgtcttttat caggtcttcc ggcccaccac
      841 ggtccccgac tggctcaccc ccgcaagcga gttgatgcgc gatgagacca tgccgagccg
      901 cctggtccag cactacgacg cgggcagcac gcgcgatgcc ctggtttgga tggaaggccc
      961 gattctgccg ggcagggctg agccggacgg ccagtccgtg gtcgtggggg cccggcaagg
     1021 ccggtacagc gagcaaaacg gcgtccgccg gctggtgctc gcagtggacg gtacggagat
     1081 cgaattggac gggcaggcga acgtcacccg cgaggtgctg attgaggttg ccgcgtcggc
     1141 gcagtctgtg ccgatgcggg tcgcagtccg gctcgacgct acggacgcgc cggtgctgcc
     1201 gcccgcgcaa ccggccctgt ttccgctgag ttttgccgac ccgctgcacg ggtgggcgct
     1261 ggcgtcctgt ccggggaatg cggcatgcgc gccggaaatc cgcgccaccg tggacggagg
     1321 cgaaacgtgg cagccggcgg gtcccgcccc cgccaacgcg tcccagttgc gctttaccac
     1381 gccgcgcgac ggctgggcgt tcgatccggg cttctgggcg acccacgacg gcgggcgcac
     1441 ctggacggcg aatcccgtgc tgccggcggt gtccgcactg gaggtcgccg gcgatacggt
     1501 ctgggcgatt gcggcaacgc ccacaggcac cgtggccccg gcggcgacgc tatacgtgtc
     1561 cagcgatagc ggggccacct ggcagaccgt gagccaccca cccatcaccg ggcctgcctg
     1621 gcaactggtg cgggccgggc cggatgcggg ctggatcctc tcctggggca actacggata
     1681 cccgcggcaa gggccaatcg gcactatcag cgcgacccag gacgcgggcc gcacctggca
     1741 gccgctgccc gatccctgtg ttggggtcgg cggggttgaa gatcggctgg ccgccgccga
     1801 tgcgcacgaa ctctggctgc tgtgcggggc gctgccggcc agcggcgcag cggaggaaaa
     1861 agcgttgttc cagtccaccg acagcggggg acactggacc ctcaatgcgg cctgggagtc
     1921 ggggcgccac gagttgcccg cggggcaagt ggtcgatctg gccgtgcccg tgccgggcca
     1981 cccctggatc gtggcaatgc gccccggctc ggtgtaccgc ccgaccgcgg caggtcagcc
     2041 ctggataccc gccatcccct cggatctggc gagtcccggc gaccgctctg tcggttccgt
     2101 gcagtttatc gacgcgcagc acggctggtt cagcacggcg ggcgacctgt tccgcaccca
     2161 ggatggcggg caaacgtggg acatgttacc ccatccttag cgggtgggcg acaagacgga
     2221 gattcttcgc tgcgctcaga atgacagggg gccaagggct tgtcactctg agcggagcga
     2281 agagtctcgg cggggacgag acggagattc tgcacggcgc tcagaatgac ggggggccag
     2341 gaggccgcgt gactatgacg ggggccaagg gattgtcact ctgagcgcag cgaagagtct
     2401 cggcggcgcg gctacttcag gcgcttgatg atgtgccagc cgaaggggct ggtctgcggg
     2461 tcgaagtcgg cgatgccgat ctggccgacc tgaagggtga agccgacgtt gccgaaggcg
     2521 ggcaccatgc cctcgcgcgg gtactcgccg tccgccgggg gcacgccgag gttcgacatc
     2581 gagtagatgc cgggcggcga gtcgtcggtg ttgtcgcgca ccagttggtc gaagtcggcg
     2641 ccgctcttgg ccttgtccag cagttgatag gccagcgtct tggcctgctc ctgggtgcgg
     2701 gtgatgggct tccccggcac gctgccctgg aagccgatca ggatgtgctg gacggtgata
     2761 tgctccggcc cattggcgcc gccgcccctc gcggcgggcg cgggcgtggg gggcaccggc
     2821 gtggcggtgg gcgcggcctc accgcacccg gccagcaggc ccacggccag cagcaatccg
     2881 atcagtatcc gcaatttcat gtaacgctcc tatctccatc gttcgattcg tcgtgtgccc
     2941 gcgccgcggc aaggggcggc ggcggggagg tagggcaacc tctcccccgg cccctccccg
     3001 cggcggggag gggagtgacg tctcctcatg ccggcgattc cccccttccc gccgcgt
//
LOCUS       DASUYC010000879         2754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1760109, whole genome shotgun sequence.
ACCESSION   DASUYC010000879 DASUYC010000000
VERSION     DASUYC010000879.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1760109"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VF276_14410"
     CDS             <1..552
                     /locus_tag="VF276_14410"
                     /inference="COORDINATES: protein motif:HMM:NF017672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006672 - ceramide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ceramidase domain-containing protein"
                     /protein_id="HEX6608094.1"
                     /translation="FYALLYGGLVVFLGPASMFFHASLRAWGGWIDLVSMMLYSSFLL
                     VYDLVRIRRAAWRVFLAIYLPLVAVQGLADALVPKSGQLLFGLGIVAVLVLEARILRG
                     QVPGLRRRGRPWFAAALGLFAGAYGGVWLWSNTAGPLCDPDSLLQGHAVWHLLCAAST
                     LCLYQYLRTEQRAGPPAGMAAAA"
     gene            697..1047
                     /locus_tag="VF276_14415"
     CDS             697..1047
                     /locus_tag="VF276_14415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608095.1"
                     /translation="MNARKHAQQIGMGEVSLHMWAGAMELAEAANAALAALTASAPAR
                     PATPALTGAVESTAMQVSPLLASAVEDGSNLEMDWLWLATKVADRTERRYCLLRALAI
                     NPTSEMARRELQRL"
     gene            complement(1065..>2754)
                     /locus_tag="VF276_14420"
     CDS             complement(1065..>2754)
                     /locus_tag="VF276_14420"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6608096.1"
                     /translation="ALVDKSLVHRVATGGSRAGGRYEVPEVLRQYAAEQLDEAGETAA
                     TAARHADYYLAFVAERSPDLRGAGQLAALAALDQEIEQIRVAWRWAVSVADRADEPPP
                     DHHADEPRPRLSRPVQGRRHPTRAPEVDDGTPWAALGRAALGLFHFYDMRSWFGEGAA
                     AFTAAATALAPAAENPQAAPIYGMVLARQGWFTFHLGRQAEAKQLLERSLAVLRPLGP
                     SPDQVFALNYLGVICAYLGEYGPARTWAEEGLAMAQAIGDRYGQAVACNILGQTAYEC
                     AEYPAAQNWSQQSYMIEQDLGNRWSMAFSLTNLGKVAFVVGEYQSARWFFTQSLEIRQ
                     EMGDTRGVAICYIRLGDVAVALGNPAEAQDRYAQSLALFREIGNQWGLAEALINQGRL
                     AAGTGDDTTAVRRLQEALQLALHTRSVPQVRAILAATAPIARRHRQAAWADELDQLVA
                     APPEGVDAYRRVGAHLLGWYPEAAPAGNGAHPAAPQAAAPGATGPTSYPAGLTAREAE
                     VLRLVAQGLTDAQVAEQLVVSRRTVSTHLTAIYGKLGVNTRSAATRFAMEHGLA"
BASE COUNT          370 a          959 c          999 g          426 t
ORIGIN      
        1 ttctacgcgc tgctgtacgg cgggctagtg gtgtttctgg gcccggccag catgttcttc
       61 cacgcgtcgc tgcgggcctg gggcggctgg atcgacctcg tctcgatgat gctctacagc
      121 agctttttgc tcgtctacga cctggtgcgc atccggcgtg ccgcgtggcg ggtgttcctg
      181 gcgatctacc tgccgctggt ggctgtgcag gggctggccg acgccctggt gcccaagagc
      241 gggcaactgc tgttcggcct ggggatcgtg gcggtgctgg tgctggaggc gcgcattctg
      301 cgcgggcagg tgccgggctt gcggcggcgg ggccggccct ggttcgcggc ggcgctgggc
      361 ctgttcgccg gggcctacgg cggcgtctgg ctctggtcga acacggccgg cccgctctgc
      421 gacccggaca gcctgctgca aggccatgcc gtctggcacc tgctgtgcgc ggccagcacc
      481 ctctgcctct accagtacct gcgcaccgag cagcgcgccg ggccgcccgc cggcatggcg
      541 gcggcggcgt agtgcttacg tacccttacg tagacccgcg cgagcgcagg ctacgtaagc
      601 caaaaccgta attcccccga tgcgccccgc gttgcgcggt agtacagtag agccatcgac
      661 atgatggacc ggaccgcgca acagaggagc aacacgatga acgcacggaa gcatgcgcaa
      721 cagatcggca tgggcgaagt ctcgctgcat atgtgggcgg gcgccatgga actggctgag
      781 gcggccaacg cggccctggc ggcgctcacc gcgagtgccc cggcgcggcc cgctaccccg
      841 gccctgaccg gtgcagtgga gagcaccgcg atgcaggtct cgccgctgct ggcttccgcc
      901 gtcgaggatg ggtcgaacct ggagatggac tggctgtggc tcgccacgaa ggtggcggac
      961 cgcaccgagc ggcgctattg cctgctgcgt gccctggcga tcaacccgac cagcgagatg
     1021 gcccggcgcg agttgcagcg cctctaaggc cccgcggtcc ggtctcaggc gagaccgtgt
     1081 tccatggcga agcgggtggc ggcgctgcgc gtattgacgc ctagcttgcc gtagatggcc
     1141 gtgaggtggg tgctaacggt gcgccggctg accacgagtt gctcggccac ctgggcgtcg
     1201 gtgaggccct gggcgaccag gcgcaacacc tcggcctcgc gggccgtcag cccggcgggg
     1261 taggaggtgg ggccggttgc ccctggtgcg gcggcctgcg gcgcggcggg atgcgccccg
     1321 ttccccgccg gggcggcctc gggataccag ccgagcaggt gcgcgccgac ccgccggtag
     1381 gcgtccaccc cttccggggg cgcggcgacc agttggtcca actcgtcggc ccaggccgcc
     1441 tggcgatggc ggcgggcaat cggcgccgtg gcagccagga tggcccgcac ctggggcacc
     1501 gaccgcgtgt gcagcgcgag ttgcagcgcc tcctgcaacc ggcgcacggc ggtggtatcg
     1561 tcgccggtgc ccgccgccag ccgcccctgg ttgatcaacg cctcggccag cccccactga
     1621 ttgccgatct cgcggaacag cgccaggctc tgcgcgtagc gatcctgggc ttcggcgggg
     1681 ttgcccaacg ccaccgcgac atcgcccagc cggatatagc agatcgccac gccccgcgta
     1741 tcgcccatct cctgccggat ctccaggctc tgcgtgaaga accagcgcgc cgactggtat
     1801 tcgccgacga cgaaggcgac cttgccgagg ttggtcagcg agaaggccat gctccagcga
     1861 ttgcccaggt cctgctcgat catatagctc tgttgcgacc agttctgcgc ggcggggtac
     1921 tcggcgcatt cataggccgt ctggccgagg atattgcagg cgacggcctg gccgtagcgg
     1981 tcgccgatag cctgggccat cgccagccct tcttcggccc acgtgcgggc cggaccgtac
     2041 tcgccgaggt aggcgcagat cacgcccagg tagttcaggg caaacacctg gtcgggactc
     2101 ggccccagcg ggcgcaagac ggccaggctg cgctccagta gttgcttggc ttcggcctgg
     2161 cgcccgaggt ggaaggtgaa ccagccctgg cgggcgagca ccatgccgta gatgggcgcc
     2221 gcctgcgggt tctcagcggc gggggccagc gccgtggccg ccgcggtgaa agcggcggct
     2281 ccttcgccga accaactccg catgtcgtag aagtggaaca gccccaacgc ggcgcgcccc
     2341 agggccgccc acggcgtgcc gtcatccacc tccggggcgc gggtggggtg ccggcggccc
     2401 tggaccgggc gcgataaccg cggtcggggc tcgtccgcat ggtggtccgg cggcggctca
     2461 tcggcgcgat ccgcaacgct gacggcccag cgccaggcca cgcggatctg ctcgatctcc
     2521 tggtcgagcg cggcgagcgc ggcgagttgc ccggcgcccc gcaggtcggg gctgcgctcg
     2581 gccacgaagg ccaggtaata gtcggcatgt cgggcggcgg tcgccgccgt ctcgcccgct
     2641 tcgtcgagtt gttccgccgc atactggcgc aagacttccg gcacctcgta gcggcccccg
     2701 gcgcggctgc cgcccgtcgc cacccgatgc accagcgatt tatcgaccag ggcc
//
LOCUS       DASUYC010000880         2135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1555133, whole genome shotgun sequence.
ACCESSION   DASUYC010000880 DASUYC010000000
VERSION     DASUYC010000880.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1555133"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2135)
                     /locus_tag="VF276_14425"
     CDS             complement(<1..>2135)
                     /locus_tag="VF276_14425"
                     /inference="COORDINATES: protein motif:HMM:NF012433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HEX6608097.1"
                     /translation="AVTVPALPPSGSYAPGSTLPVQLALTDTGSNPATAEVLLRLRAL
                     GDETAPATGSPPTASWPAGVPAPPITGASTEAPVFWNAGLALDGSGQLTTTVRLPVDA
                     GVWNLDAWALTPSGVSHSRALVRTLPPLAVQWAMPESLVQGDNTTAVATITNNQETVA
                     NVSLHLQLTGDHALDLTSPQEQSFRLAPGETRTATWTMRAPAAGTVQVSLDAAWGAPG
                     APGLTVHRGQLVVVAPYGIVDEQAHAGTVGDSESINMTLPGDVDPATARLDIYCAPTV
                     PGTLIDAVADLTPSGGAGLRGRVDEVAGRLAAGATLRAFYRSQGLDADDLPTDLAANS
                     LLDLQYLYSAQGADGGWGPWPGAPSDLRTTLAVVDALQQFGAEPDGALDMPTVARGMA
                     WVQRAAVLPVLPGAAVDPALDPAVADATARAIYLLARNNQADTTTVNRLLAAEPRLGP
                     RSRAYLALILTQTGRAEEGRLIVARLAAAADTSADPIVLDALLTADTTGVRGPVGDIA
                     RALLGARVGPVWATADETAAAVRALSHYAAAVPETATAGSYRILVNGQLVREVAAGPA
                     ATNGGRTRLTVSGARLHSGTNTVRLETSGARLYYSLRVQAPLAASEHPITTRQSDSAP
                     LGLLRVYEPLAPSAVHVALTMTVGIPMGPMRLDDPLPAGLARLPGLRFVRLDGPPDAP
                     IAVDLADAIQAVDHMPDGRGLRVWFGPLP"
BASE COUNT          276 a          759 c          812 g          288 t
ORIGIN      
        1 gggcagcggg ccgaaccaca cgcgcagccc gcgcccgtcg ggcatgtggt ccaccgcctg
       61 gatggcgtcc gcgaggtcca cggcgatggg cgcgtccggc gggccgtcga ggcgcacgaa
      121 gcgcagcccc ggcaaccgcg ccagcccggc gggcagcggg tcgtccaggc gcatcggccc
      181 catcgggatt ccgacggtca tcgtcagcgc cacgtgtacg gcggacggtg ccagcggctc
      241 gtacacacgc agcaggccca gcggggcgct gtcgctctgg cgggtggtga tgggatgctc
      301 gctcgcggcc agcggcgcct gcacgcgcag gctgtaatag agccgcgccc cgctcgtctc
      361 caggcgcacg gtattggtgc ccgaatgcag ccgggcaccg gacacggtca gccgcgtgcg
      421 cccgccgttg gtcgccgcgg gccccgcggc cacttcgcgc accaactggc cgttgaccag
      481 gatgcgatag ctgcccgcgg tcgccgtttc gggtactgcc gcggcgtagt ggctgagcgc
      541 gcgcaccgcc gccgccgtct catcggcagt ggcccagacg ggccccacgc gggcgcccag
      601 cagcgcgcgg gcgatgtcgc ccacgggacc gcgcaccccg gtggtatcag cggtcagcag
      661 cgcgtcgagc acgatggggt cggcgctggt gtcggctgcc gccgcgagcc gggccacgat
      721 caagcgcccc tcctcggccc ggcccgtttg cgtcagaatg agggccaggt aggcgcggct
      781 acgcggcccc agccgcggct ccgccgccag cagccggttg acggtggtgg tatcggcctg
      841 gttgttgcgc gccagtagat agatggcccg cgccgtggca tcggctaccg cgggatcgag
      901 cgccgggtcg acggcggccc ccggcaggac gggcaagaca gctgcccgtt gcacccaggc
      961 catgccgcgc gccaccgtgg gcatatcgag cgcgccgtcg ggttccgcgc cgaactgctg
     1021 cagggcgtcc accacagcca gcgtggtgcg tagatcggag ggggcgcccg gccagggccc
     1081 ccagccgccg tcggcgccct gcgcgctgta gagatactgc aaatcgagca ggctgttggc
     1141 ggccagatcg gtgggcagat cgtccgcatc gaggccctgg ctgcgataga acgcgcgcag
     1201 tgtggccccg gcggcgagcc ggcccgcgac ctcatcgacc cgcccgcgca gccccgcgcc
     1261 gccgctgggc gtcaaatcgg ccacggcgtc gatgagtgtg cccggcacgg tgggcgcgca
     1321 atagatgtcg aggcgcgccg tcgccggatc gacatcgccc ggcaaggtca tgttaatcga
     1381 ctcgctgtcg ccgaccgtgc ccgcatgcgc ttgctcgtcc acaatgccgt agggcgccac
     1441 gacgaccagc tggccgcggt ggaccgtgag accgggcgca cccggcgccc cccaggcggc
     1501 atcgaggctg acctgcaccg tgcccgcggc gggcgcccgc atggtccagg tagcggtgcg
     1561 ggtttcgccg ggggccaggc ggaagctttg ctcctgcggg ctggtcaggt cgagcgcgtg
     1621 gtcgccggtc agttgcaagt gcaggctaac gttggcgacc gtctcctggt tattggtgat
     1681 ggtggcgacg gcggtggtgt tgtcgccctg gaccagcgat tccggcatgg cccattgcac
     1741 cgccagcggc ggcagggtgc gcaccagcgc gcggctgtgg ctcacgccgc taggggtcag
     1801 cgcccaggcg tcgagattcc agactcccgc atcgacgggc aggcggaccg tcgtggtcaa
     1861 ctggccgctg ccgtccaggg ccagcccggc gttccagaac accggcgctt cggtggatgc
     1921 gccggtgatc ggcggcgccg gcaccccggc gggccagctt gcagtgggcg ggctgcccgt
     1981 ggccggcgcg gtttcgtcgc ccagggcgcg gaggcgcagc agcacttcgg cggtcgcggg
     2041 gttgctgccg gtatccgtca gggcgagttg caccggcagc gtactgccgg gcgcatagct
     2101 gccggacggt ggcagcgcgg gcacggtcac ggcca
//
LOCUS       DASUYC010000881         2316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1489591, whole genome shotgun sequence.
ACCESSION   DASUYC010000881 DASUYC010000000
VERSION     DASUYC010000881.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1489591"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="VF276_14430"
     CDS             <1..901
                     /locus_tag="VF276_14430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6608098.1"
                     /translation="QMAYYRLPAGVPAVLDLHNVEYEVLGRVAAGGGAPLRRLYNWAE
                     YRKFRRAEPQLWRRFATLLVTSERDGALVRRHLPGARPVVVPNGVDTGFFRPPPPGAG
                     PEPGAGPQIVFTGMMAYYPNHDGALYFAEAIWPAIRQALPAARWLIVGVEPPSEVRAL
                     DGTAGITVTGRVDDVRPYVWRSGASVVPLRMGGGTRLKIVEALAMGQAVVSTAVGCEG
                     IAVDPGRNLLVADDPAAFAAAVVDLLRDPARAAAIGAAGRTLAEREYSWPVVVRPMLE
                     AWAAATTSGQWSVVSGQPSAVRS"
     gene            1002..1619
                     /locus_tag="VF276_14435"
     CDS             1002..1619
                     /locus_tag="VF276_14435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608099.1"
                     /translation="MVNEGRRGDWALRLGVALAGAVLLLATLWLPGRSVPHAAAAPAA
                     LESVISGTVAWKDSAEPVSGVQVVLKDAVQGTVVGQTTTDAEGVYTITAGVGNWLVDV
                     PSTARYWGYAQTVTSFPHTDYHLDFGITVRPPEAPPAAPPAAATPGATPGTTPAAGAK
                     PGTMPPAGHPTSLGLWLVLGTLLALLGGALRGAGQRRYRAAKETC"
     gene            1622..>2316
                     /locus_tag="VF276_14440"
     CDS             1622..>2316
                     /locus_tag="VF276_14440"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX6608100.1"
                     /translation="MSDVLSILGFLAGALLLAAEVVLVLLVGYLLFLTAAAVVVWLTR
                     PGERLGADGPLRQATAEAGRVPAPGRWPAPRTRFTVLVPAHDEELLIRRVVGSLLAVD
                     YPPDLYQVHVIADNCTDRTAELARAQGAVVHERQDAARRGKGYALRWLFAQLLRDPAS
                     GDAFVIVDADSIVNQGFLRALDRRLRGDTVAIQAYGTVLNVAASWGTALRYVAFALLH
                     YLRPLGRKLFDGSA"
BASE COUNT          283 a          770 c          893 g          370 t
ORIGIN      
        1 gcaaatggcc tattaccggc tgcccgcggg cgtgccggcg gtgctcgatc tgcacaacgt
       61 cgaatacgag gtgctgggcc gggtggcggc gggcggcggc gcgcccctgc gccgactcta
      121 caactgggcc gagtaccgta aattccgccg cgccgagccg caactctggc ggcgcttcgc
      181 cacgttgctg gtgacttcgg agcgcgacgg cgcgctggtg cgccggcatc tgcctggcgc
      241 gcgcccggtg gtcgtgccca acggggtgga taccgggttc ttccgcccgc cgccgccggg
      301 tgcgggaccg gagccgggcg ccggcccgca gatcgtgttc accggcatga tggcctacta
      361 cccgaaccac gacggcgccc tctacttcgc cgaggcgatc tggccggcca tccggcaggc
      421 gttgcctgcg gcccgctggc tgatcgtcgg cgtggagccg ccgtccgagg tgcgcgccct
      481 cgacggcacg gcgggcatca ccgtgaccgg gcgggtggac gatgtgcgtc cgtatgtctg
      541 gcgcagcggc gccagcgtcg tgccgttgcg catgggcggc ggtacccggc tgaagatcgt
      601 cgaggcgctg gcgatgggcc aggcggtggt ctcgacggcg gtgggctgcg agggcatcgc
      661 cgtggatccg ggccggaatc tgctggtcgc cgacgacccg gcggcttttg cggctgcagt
      721 cgtagacctg ctgcgcgatc cggcgcgggc ggcggctatc ggggcagcgg ggcgcacgct
      781 ggccgagcgc gagtatagtt ggcccgtggt ggtccggccc atgctggaag cgtgggcggc
      841 ggcgacgacg agtggtcagt ggtcagtggt cagtggtcag ccgtcagccg tcaggagtta
      901 gtaagtgcga taaggcgcag ggccggtaag gtatgccgcg cggcgtgcgg agaaatcgat
      961 tggatattca atgggggccg tttagaaggg tgactacgcg aatggtgaat gagggacggc
     1021 gcggcgattg ggcgctgcgg ctgggggtgg cgctggcggg cgcggtgctg ctgctggcga
     1081 cgctgtggtt gccggggcgt tcggtgcccc atgcggctgc cgcgcccgcg gccctggagt
     1141 cggtgatcag cggcactgtt gcctggaagg acagcgcgga gccggtgagc ggcgtgcagg
     1201 tggtcttgaa ggacgcggtc cagggcacgg tggttgggca gaccacgacc gacgccgagg
     1261 gcgtctacac gatcacggcg ggtgtgggca actggctcgt cgacgtgcct tccaccgcgc
     1321 gctactgggg ctacgcgcag accgtgacct ccttcccgca taccgactat cacctggact
     1381 tcgggatcac ggtgcgccca ccggaggccc cgccggcggc cccacctgcc gccgccacac
     1441 cgggtgcgac tccgggtacg acgcctgccg ccggcgccaa accgggcacg atgccgcccg
     1501 ccgggcaccc cacatcgctc ggcctgtggc tcgtcctggg aaccctgctc gccctgctgg
     1561 gcggggcgct gcggggcgca gggcagcgcc ggtaccgcgc cgcgaaggag acctgctaat
     1621 catgtccgac gtgctgtcta tcctgggttt cctggcgggt gcgctgctgc tggcggctga
     1681 agtggtgctg gtgctgctgg tcggctacct gttgttcctg acggcggctg ccgtggtggt
     1741 ctggctgacc cgccccggcg agcgcctggg cgccgacggg ccgctgcgcc aggcgaccgc
     1801 cgaggccggg cgggtgcccg cccccggtcg ctggcccgcg ccgcgcaccc gcttcaccgt
     1861 gctggtgccc gcgcacgacg aagaactgct gatccgccgc gtggtgggca gtctgctggc
     1921 tgtcgattac ccgcccgacc tgtaccaggt gcatgttatc gccgacaact gcaccgaccg
     1981 gaccgccgaa ctggcccgcg cgcagggcgc cgtggtgcac gagcggcagg acgccgcgcg
     2041 gcgcggcaag ggctacgcgc tgcgctggct gttcgcccaa ctgctgcgcg acccggcgag
     2101 cggcgatgct ttcgtgatcg tggacgccga ctccatcgtg aaccagggct ttttgcgggc
     2161 cctcgaccgg cggctgcgcg gcgacaccgt cgctatccag gcctacggca cggtgctgaa
     2221 tgtcgcggcg tcgtggggca cggcgctacg ctacgtggcc ttcgcgctct tgcattacct
     2281 gcggccgctg gggcgcaagc tgttcgacgg gtcggc
//
LOCUS       DASUYC010000882         2432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_917341,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000882 DASUYC010000000
VERSION     DASUYC010000882.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917341"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VF276_14445"
     CDS             <1..555
                     /locus_tag="VF276_14445"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX6608101.1"
                     /translation="YSAGGVVCAGAIVRRPDLWAGGLLRQTVLHMLRYEYGAGGPPNV
                     PEFGSTLTRRGARALRAMDPFTALRPGVRYPPMLALVGANDPRVPIWHAMKWVARLQD
                     AAAGRPLAALLRVEFHAGHGHGSTRRQIIRELADRYAFALAACRVAQRAPDAAPHPIS
                     AALPAHPPLTRLADRAETGEAPAA"
     gene            complement(569..1180)
                     /locus_tag="VF276_14450"
     CDS             complement(569..1180)
                     /locus_tag="VF276_14450"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX6608102.1"
                     /translation="MTDLSADRAAPGAEAGKRSESRLERYLRMQRLVLHVRLGAAQAL
                     QRCIPYSLFGNVRSAIYRVCGFRHIARKVYLAGPLDIRGDGDIYPRLHIGAHTFINTP
                     CFIDLNAPVHIGEHVGIGHHVRIITSNHEIGPPHHRMGAIKAAPVTIGAGAWIAAGAM
                     ILPGVTIGPGALVMAGAVVTKDVPANAKAAGMPAQVVGALPVE"
     gene            1377..1841
                     /locus_tag="VF276_14455"
     CDS             1377..1841
                     /locus_tag="VF276_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608103.1"
                     /translation="MAVVSLFLAPARVSAEVDSEGNVYEGRVGEDPGSPVGMVDAATG
                     MVYRRRRGRQLRRVGEVDAEGVVYVLERRGFWPWARGGQPVPILEIDSAGDIYDLTTV
                     NRRPRIGEVDGQGSVFYAGGAALLLWLLHRRERISRDTYLWYQMSAGQHGLR"
     gene            2073..>2432
                     /locus_tag="VF276_14460"
     CDS             2073..>2432
                     /locus_tag="VF276_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX6608104.1"
                     /translation="MRADIVPGARFPDYELTDHTGTRRRLSELQGNDPLILVLSRGHF
                     CPKDHQQHLDLASFYPKITVAYTRIVTIATDNVLELNEFRDAVGAQWPFLSDVARKVQ
                     QDLDIQEYTDPHHNPMIP"
BASE COUNT          359 a          843 c          845 g          385 t
ORIGIN      
        1 tatagcgccg gcggggtggt ctgcgcgggg gccatcgtgc ggcgcccgga cctgtgggcc
       61 ggtgggctgc tgcgccagac agtgctgcac atgttgcgct acgagtacgg cgccggcggc
      121 ccgcctaatg tgcccgagtt cggatccacc ctgacccggc gcggggcgcg ggccttgcgg
      181 gccatggacc cctttaccgc cctgcggccc ggcgtgcgct atccgcccat gctggccctg
      241 gtcggggcga acgatccgcg cgtgccgatc tggcacgcca tgaagtgggt cgcccgcctg
      301 caagatgccg ccgccggccg gcccctggcg gcgctattgc gcgtggagtt ccacgcaggc
      361 cacggccacg gctccacccg gcggcagatc atccgcgaac tggccgaccg ctatgcgttt
      421 gcgctggccg cctgtcgcgt tgcgcagcgc gcgcccgatg ccgccccgca tccgatcagc
      481 gcggcactgc ccgcccaccc gccgctcacc cggctggcag acagggcgga gaccggcgaa
      541 gcgcctgctg cctgaccggg ggcgggggct attccacggg cagtgcgccg acgacctggg
      601 cgggcatccc cgccgccttg gcgttagccg ggacatcctt cgtgaccacc gccccggcca
      661 tgaccagggc gccggggccg atggtcacgc ccggcaagat catcgcgccc gccgcgatcc
      721 acgcgccggc gccgatcgtc accggcgcgg ccttgatcgc ccccatgcgg tggtgcggcg
      781 gcccgatttc gtggttgctg gtgatgatcc gcacgtggtg gccgatgccc acgtgttcgc
      841 cgatgtggac cggcgcattg aggtcgataa agcacggggt gttgatgaag gtgtgcgcgc
      901 cgatgtgcag ccggggatag atgtcgccgt cgccccggat gtccagcggc ccggcgagat
      961 agactttgcg cgcgatgtgg cggaatccgc agacccggta aatggcgcta cgcacgttcc
     1021 cgaacaggct gtatgggatg cagcgctgaa gcgcctgcgc cgcgcccaaa cgcacatgga
     1081 gtacgagccg ttgcatgcgc aggtagcgct ccagccggga ctcgctgcgc ttgccggcct
     1141 ccgcgccggg ggccgcccga tccgccgata gatctgtcac actcgcgtcc tttcttcgcc
     1201 tcgctatcac tgctgcaccc tgggccggca ccgctgcggc ccggccaaac gatcctattg
     1261 taccgtaccg cgcaagatcg cgcgggccgg cgcaactggc atggtttgtg cgcgatcccg
     1321 gatgcaagcg ccgcgccaca ttgggacgcg catcacggag gaaaggaagg ccgctggtgg
     1381 ctgtcgtatc gctgttcctg gcccccgcgc gggtgagtgc ggaggtcgat tccgaaggca
     1441 acgtctatga gggacgggta ggtgaagacc ccggcagccc cgtgggcatg gtggacgccg
     1501 ccaccggcat ggtctatcgc cggcggcggg gcaggcagtt gcgccgcgtg ggagaagtcg
     1561 atgccgaggg cgtcgtctat gtgctggagc gccgggggtt ctggccctgg gcgcgcggcg
     1621 ggcagccggt gcccatcctg gagatcgaca gcgccggcga catctacgac ctgaccacgg
     1681 tgaaccggcg cccgcgcatc ggcgaggtgg acgggcaagg ctcggtgttc tatgcgggcg
     1741 gcgccgcgct gctgctctgg ttgctgcacc gccgggagcg catcagccga gatacctacc
     1801 tctggtacca gatgtccgcc gggcagcacg gcctgcggtg acgggcgccc ccgcccggag
     1861 gctcaagcct ccggctcgcg ttacgaaggc cgcctgcgcg gcctggcttg atgttcgcgg
     1921 gcggggtggc cgcgctgtaa cggctcgcac aggttttgcg cgcaaagcgt ggtataagta
     1981 cgatgtgccg cttgctgtgc gcaggggcac ggtcatcgac agttgccaac ggcgcggccc
     2041 cgttcgttgc accttgctta aaggagaaga tcatgcgcgc agacatcgtt cccggcgccc
     2101 ggtttcccga ctacgaattg accgatcaca ccggcacgcg gcgccggctc agcgagttgc
     2161 agggcaacga cccgctcatc ctggtgctgt cgcgcgggca cttctgcccg aaggaccacc
     2221 agcagcacct cgatctggcg agcttctacc ccaagatcac cgtcgcctac accaggatcg
     2281 tcaccatcgc caccgacaac gtgctcgaac tcaacgaatt tcgtgacgcg gtgggcgcgc
     2341 aatggccttt cctgtccgac gtggcgcgca aggtgcagca ggatctcgac atccaggagt
     2401 acaccgaccc gcaccacaac cccatgatcc cg
//
LOCUS       DASUYC010000883         2188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_848247,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000883 DASUYC010000000
VERSION     DASUYC010000883.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848247"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VF276_14465"
     CDS             complement(<1..329)
                     /locus_tag="VF276_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608105.1"
                     /translation="MSRRTRRSRRKRLLPAGPRWTLLARGVRRQARAGVRTARKRRNE
                     LAITLLYAIAGALCLALAALLVAPSPTPAVEAATRLPEATPPVFRATVIGAVVLAGIG
                     VVLFGLAL"
     gene            complement(326..2134)
                     /locus_tag="VF276_14470"
     CDS             complement(326..2134)
                     /locus_tag="VF276_14470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608106.1"
                     /translation="MQRGLGVVERRLRGQEAVQGAPLAAAVALVPAIVLALAARLTPI
                     LPPPAVAAVAVTLLILAVAGRAAYAWLRTRPRLGTARRADALLALDEKAATAVEAQER
                     PRPGDAPLLRSAQLADATASLERALPDLPARVPLAPGRTALILPVLLLAAFGAALLAP
                     NPLQGYWDQQNAIRQQQQAEAEKIEQLRKDLAARPGANDPARQELLAQLDQLHHDLQA
                     GTLSRDQAMARLSDAEAALQKQIDPRAPGEQAAMDALARQLAEQPGALGDAGKALQRG
                     DTDQAAKDLQRAGADAAQMSPADRQALAEGLRQAAAEAAANDPALAQSLEDAARAAQG
                     NDPQATQQAFDNLAQQAAAAGQQQMTQQELNQALAQLQASKNNIASSGQPQTAGTPGP
                     TAVPGTAVALGTPAGTPLPAGTPIAGGTPLALGTPLALGTPVSLAGTVVPGTFTGTGT
                     PVLVPGTPGQGTPVAVQGQGQGQGQGQGQGQGQGQGQGQGQGQGQGQGQGQGQGGGVG
                     GSGAGNQHTEPVVVPPSTLNAQGTPVGVAARPNSGETGVTGVNVDPATGNATVPYQQV
                     YGTYNDSAQKTLSTTYIPSGMKDLVRRYFTAIEPAP"
BASE COUNT          218 a          800 c          842 g          328 t
ORIGIN      
        1 agggccagcc caaacaagac cacgccgata cccgccaaca ctaccgcccc gatgaccgtg
       61 gcgcggaaga ccgggggcgt ggcttcgggc agccgggtcg ctgcttcgac ggcgggcgtg
      121 ggggagggcg cgacgagcaa cgcggccagc gccaggcaca gcgcgccggc aatggcgtag
      181 agcagggtga tcgccagttc gttgcgccgt ttgcgggccg tgcggactcc ggcgcgcgcc
      241 tggcggcgga caccgcgcgc cagcagcgtc cagcgcggcc ctgccggcaa gaggcgcttg
      301 cggcggctgc ggcgggtgcg gcggctcacg gcgcgggctc gatagccgtg aagtagcggc
      361 gcaccaggtc cttcatgccc gacgggatgt aggtcgtgct cagcgtcttt tgcgcgctgt
      421 cgttatacgt cccatagacc tgctggtagg gcacggtcgc gttgccggtg gccgggtcga
      481 cgttcacgcc ggtaacgccg gtctcgcccg aattgggccg cgccgccacg ccgacgggcg
      541 tgccctgcgc gttgagcgtg ctcggcggca cgaccaccgg ctcggtgtgc tggttgcccg
      601 cgccgctgcc gccgaccccg ccgccctggc cttgcccctg tccctggccc tgaccttgtc
      661 cttgtccctg gccttgtcct tgcccttgtc cttgtccctg gccttgcccc tgcccctggc
      721 cctgaccttg cccttgcacc gctaccggcg taccctggcc gggggtgccg ggcaccagca
      781 ccggcgtgcc cgtcccggta aacgtgcccg gcacgacagt gcccgccagg ctgaccggcg
      841 tgccgagggc cagcggcgtg ccgagggcta gcggtgtgcc gcccgcgatg ggggtgccgg
      901 cgggcagcgg cgtgccggcg ggggtgccca gtgccacggc ggtgcccggc actgccgtcg
      961 ggccgggcgt gccggcggtt tgcggctggc cgctggaggc gatgttgttc ttgctggctt
     1021 gcaactgggc cagcgcctgg ttcagctcct gctgcgtcat ttgctgctgc ccggcggcgg
     1081 ccgcctgctg cgccaggttg tcgaaggctt gctgggtcgc ctgggggtcg ttgccctggg
     1141 cggcgcgggc ggcgtcttcc aggctctgcg ccagggccgg gtcattggcc gcggcctcgg
     1201 cggcggcctg gcgtagcccc tcggccagcg cctggcggtc tgcaggcgac atctgcgccg
     1261 cgtcggctcc cgcccgttgc agatccttgg ctgcctggtc ggtgtcgccg cgttgcagcg
     1321 ccttcccggc gtcgcccagg gcgcccggct gctcggccag ttggcgcgcc agggcgtcca
     1381 tcgccgcctg ctcgcccggc gcgcgcgggt cgatctgctt ctgcaacgcc gcctcggcgt
     1441 ccgagagacg ggccatcgcc tggtcgcggc tcagggtgcc cgcctggaga tcgtggtgca
     1501 gttggtcaag ctgggccagc agttcttgcc gggccgggtc gttcgcgccg ggccgggcgg
     1561 ccagatcttt gcgcaactgc tcgatcttct cggcctcggc ctgctgctgt tgccggatgg
     1621 cgttttgctg gtcccagtac ccttgcagcg ggttgggcgc cagcagggcg gcgccgaagg
     1681 cagccagtag cagcacgggc aggatcagcg ccgtgcgccc cggcgccagc ggcacgcgcg
     1741 cgggcaggtc cggcagcgcg cgctccaggc tggccgtggc gtcggccagt tgggccgacc
     1801 gcagcagggg cgcatcgccg ggtcgcgggc gctcctgcgc ctcgacggcg gtggccgcct
     1861 tctcgtccag cgccagcagg gcatcggcgc ggcgggccgt gcccaggcgc gggcgcgtac
     1921 gcagccaggc gtacgcggcg cgccccgcca ccgcgaggat caacagcgtc acggcgacgg
     1981 cggccaccgc gggcggcggc aggatgggcg tgagccgcgc ggccagcgcc agcacgatcg
     2041 ccggcaccag cgcgacggct gccgccaggg gcgcgccctg cacggcctcc tgcccgcgca
     2101 ggcggcgctc gacgaccccc aggccccgct gcaacgcggc atagggcccg gcgggcgtgg
     2161 tgcccggcgg cagggtcggg tgcattta
//
LOCUS       DASUYC010000884         2158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_232526,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000884 DASUYC010000000
VERSION     DASUYC010000884.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232526"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2158)
                     /locus_tag="VF276_14475"
     CDS             complement(<1..>2158)
                     /locus_tag="VF276_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming)/trehalose-phosphatase"
                     /protein_id="HEX6608107.1"
                     /translation="PFTVTKEEGWLAFNASAGGLVTGLSGYLQSGEAGAEGEANYIWV
                     GWPGLSISDPALQAEVRARAAAEYHSYPVFIAEEEMDRFYHGFCNSTIWPLFHYFPSY
                     ATFVEADWENYKKVNQVFCDAVMEVLQPDDIVWVHDYHLMLLPQMIRARMPEVPIGFF
                     LHIPFPAYEVFRLLPRAWRTGILNGLLGADLVGFHTYDYTQYFVRSVLRVMGYDHNLG
                     QIALGDRLVQADTFPMSIDYDKFHDAVATPEVQQERARLDASLGDKKIVLSIDRLDYS
                     KGIINRLEGYEVFLKHNPAWRGKVVLGLVVIPSRVGVNQYQETKTQIEELVGRINGKF
                     GTIHWTPIIYQYRSLPFSELIALYSRSDVALITPLRDGMNLIAKEYVAARAGETGVLI
                     LSEMAGAATELGEAVLINPTSRLEIAQGIREALKMPQEEQIRRNRLMQARLGDYTVVT
                     WAADFITQLLAAVRAQEKLQVKQLTEAVRARMLADYQASARRLLFLDYDGTLVPFAPR
                     PEQARPTAEVLDLLRGLAADPANAVVLISGRDKDTLESWFGDLPINMVAEHGVWVRER
                     EDGWHMVQRLSDEWKPQIRPLLDVYAARLPGSFVEEKPYALAWHYRQADPDLGRVRAQ
                     ELQDLLLQLTGNIDVRVLQGNRVLEVKSGGVTKGTTARQWLPGTGAAPFILALGDDWT
                     DEDLFAVLPESAYSIRVGQAQSYARFTVRDYQEVRRL"
BASE COUNT          344 a          707 c          737 g          370 t
ORIGIN      
        1 ggaggcggcg cacctcctgg tagtcgcgca cggtgaagcg ggcatacgac tgtgcttggc
       61 ccacgcggat tgagtacgcg gactccggca gcacggcaaa caggtcctcg tcggtccagt
      121 cgtcgcccag ggccagaatg aagggggcgg cacccgtgcc gggcagccac tggcgggccg
      181 tggtgccctt ggtcacgccg ccgctcttga cttcgagcac ccggttgccc tgcaacacgc
      241 gcacgtcgat attgccggtc agttgcagca acaggtcctg gagttcctgc gcgcgcacgc
      301 ggcccaggtc ggggtcggcc tgccggtagt gccaggccag ggcgtagggc ttctcctcga
      361 cgaacgagcc gggcagccgc gccgcgtaga catcgagcag cgggcggatc tgcggcttcc
      421 actcgtcgct gagccgctga accatgtgcc agccgtcctc gcgctcgcgc acccagacgc
      481 cgtgctcggc caccatattg atcggcaggt cgccgaacca gctttccagc gtgtctttgt
      541 cgcgcccgct gatcagcacc acggcgttgg ccgggtcggc ggccaggccg cgcagcaggt
      601 cgagcacctc ggcggtgggc cgcgcctgct cggggcgcgg ggcaaacggc accagcgtgc
      661 cgtcgtagtc gaggaacagc agccgccggg cgctcgcctg gtaatcggcg agcatccgcg
      721 cgcgcacggc ctcggtcaac tgcttaactt gcagtttttc ttgcgcgcgc acggcggcca
      781 acaactgggt gatgaaatcg gcggcccagg tgacgacggt gtaatcgcct agccgggcct
      841 gcatcagccg gttgcggcgg atctgttctt cctggggcat tttcagcgct tcgcggatgc
      901 cctgggcgat ctccagccgg ctggtcgggt tgatcagcac agcttcgccc agttcggtgg
      961 ccgcgcccgc catctcgctc aggatcagca cgccggtctc gccggcccgc gcggcgacat
     1021 actccttggc gatcaggttc atgccgtcgc gcaggggggt tatcagcgcc acgtcgctgc
     1081 gcgagtagag cgcgatcaac tccgagaacg gcagcgagcg gtactggtag atgatcggcg
     1141 tccagtggat ggtgccgaac ttgccgttga tgcggcccac cagttcctcg atctgggtct
     1201 tggtttcctg gtactggttc acgcccacgc gcgaggggat gaccaccagg ccgagcacga
     1261 ccttgccgcg ccaggccggg ttgtgcttga ggaagacctc gtagccctcc agccggttga
     1321 tgatgccctt cgagtagtcg agccggtcga tggacagcac gatcttcttg tcgcccagcg
     1381 acgcgtcgag ccgggcgcgc tcttgctgca cctcgggcgt ggcgacggcg tcgtggaact
     1441 tgtcgtagtc gatgctcatg gggaaggtat cggcctgcac cagccggtcg ccgagggcga
     1501 tctggcccag gttgtggtcg tagcccatga cgcgcagcac cgagcgcacg aagtattggg
     1561 tgtagtcgta ggtgtggaag ccgaccaggt cggcgccgag caggccgttg aggatgccgg
     1621 tgcgccaggc gcgcggcagc aggcggaaca cctcgtaggc cgggaagggg atgtgcagga
     1681 agaacccgat gggcacctcc ggcatgcggg cgcggatcat ctgcggcagc aacatcaggt
     1741 gatagtcgtg cacccagacg atgtcgtcgg gttgcagcac ctccatcacc gcgtcgcaaa
     1801 agacctgatt gacttttttg tagttctccc agtcggcctc cacgaacgtg gcgtaggacg
     1861 ggaagtaatg aaagagcggc cagatggtgc tgttgcagaa cccgtggtag aagcggtcca
     1921 tctcctcttc ggcgataaac accggatacg agtgatactc cgcggcggcg cgggcgcgca
     1981 cctcggcctg caacgccggg tcgctgatcg agaggcccgg ccagcccacc cagatgtagt
     2041 tggcctcgcc ctcggccccc gcctcgcccg actgcaagta gccgctcagg cccgtcacca
     2101 ggccgcccgc gctggcgttg aacgccagcc agccctcctc cttcgtcacc gtgaacgg
//
LOCUS       DASUYC010000885         2255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1531079, whole genome shotgun sequence.
ACCESSION   DASUYC010000885 DASUYC010000000
VERSION     DASUYC010000885.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1531079"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            342..1046
                     /locus_tag="VF276_14480"
     CDS             342..1046
                     /locus_tag="VF276_14480"
                     /inference="COORDINATES: protein motif:HMM:TIGR03717.1"
                     /note="Members of this highly hydrophobic protein
                     family,regularly are found preceded by the yybP-ykoY
                     manganese riboswitch (see RF00080). A metal cation
                     transport function is proposed; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjbE family putative metal transport protein"
                     /protein_id="HEX6608108.1"
                     /translation="MEHVGAALFSIIMVNLLLSGDNAVVIGMAVRTLPARQQQRAMII
                     GGLGAIVLRVLLTAVAALLLRIPLLEATGGLVLAWITYRLLAPGDGTEAVRIAGEGTF
                     GAALRTIIVADLTMSIDNMLAVAAAAHGNLGLLIIGLVLSMAVILAGGSLVAVLLGKL
                     PWLVYAGGAILLLVAGNLIASDPVLKDVLGVYPWAAWGLAGALGLVVAGALIHRQRRP
                     LSPREDAPAEHDIMHV"
     gene            1179..1865
                     /locus_tag="VF276_14485"
     CDS             1179..1865
                     /locus_tag="VF276_14485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6608109.1"
                     /translation="MISSYAMLGRTRVTGLGAAKPAEAGAPPRPPVVPPALAPESAAP
                     AVPEADEAALVQRARAGDREAFAALFDRYQTPIVNYLYRVVGDWETANDLAQDSFLKA
                     YQALARTDTDLQLAPWLYRIATNTALDALRRRKRITWVPFLEEFEPPAPGGDPGTTVP
                     NQDAVHRALQAVPDDYRIALVLHLHQGLSYKEIGAALGVAPNLVAVRVFRGREKFIKV
                     YKALTGEPDR"
     gene            1862..>2255
                     /locus_tag="VF276_14490"
     CDS             1862..>2255
                     /locus_tag="VF276_14490"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX6608110.1"
                     /translation="MKCAQCRPLVSKYVDGEASQAERRQVEAHLGTCPACSALLAQYR
                     MAKAHVGNLPYYQPDARLRARLLESLDAHDRARADEIPITRRAVPAAPAPRRAHPQGG
                     RLFGTFASGFAMFLVAAAGVLVWQIAGNR"
BASE COUNT          364 a          729 c          753 g          409 t
ORIGIN      
        1 gattgttgtt ttgactaatt ttcacaaagg ggcttgactc agtagcaatt gatcacttca
       61 ttagttaaca gtgttgtctc gaattgctgc tagaaccaga gctaatctcc aaaaattgta
      121 agaattagtc aaaacaacaa actctgagcg cagcgaagag tctcgtctcc cgtgggctag
      181 cggcgagatg cttcgctgcg ttcagcagga cggtgggggc ttgtcactct gagcgcagcg
      241 aagagtctcg tctcctgcag gctggcgacg agatgcgtcc ttgcgttcag caggacaaca
      301 acgattgaga gcggcggtag agcagcgcta gaaaggatat tgtggaacac gtaggcgcgg
      361 cactgttcag tattatcatg gtcaacctgc tgctcagcgg ggacaacgcg gtggtcatcg
      421 gcatggcggt gcgcacgctg cccgcccggc agcagcagcg ggcgatgatc atcggcggcc
      481 tgggcgcgat tgtgttgcgc gtgctgctca ccgccgtggc cgcgctgctg ttgcgcatcc
      541 ccttgctgga agcgaccggc ggcctggtgc tggcctggat cacctaccgg ctgctggcgc
      601 cgggcgacgg cacggaagcc gtacgcatcg ccggcgaggg taccttcggc gcggccctac
      661 gcacgatcat cgtggccgac ctgacgatga gtatcgacaa catgctggcc gtggccgccg
      721 ccgcccacgg caacctgggc ctgctgatca tcggcctggt gctcagtatg gccgtcatcc
      781 tggccggcgg cagcctggtc gccgtgctgc tgggcaagtt gccctggctg gtatatgccg
      841 gcggggcgat attgttgctg gtcgcaggga acttaattgc ctccgatccc gtattaaagg
      901 atgtactggg cgtgtatccc tgggcggcct gggggctggc aggcgcgctg ggcctggtcg
      961 tcgccggggc gctgatccac cggcagcgcc gcccgctctc gccccgcgaa gatgcccccg
     1021 ccgaacatga tataatgcac gtgtaatatt cgcctgccct ttggcgtctt aatggtggta
     1081 atcgcgaccc gcgacgactc cccaccggga tggctgcaac ggcgcagttg gcccggtgga
     1141 gtggtcgccc cacctgtgac gctgagggct atttgggaat gatcagtagc tacgccatgc
     1201 tgggccgtac ccgtgtcacg ggcttaggcg ccgccaagcc ggctgaagcc ggggcgccgc
     1261 cccgcccgcc cgtcgttccc ccggctctcg cgccggagag cgccgcgccc gccgtccctg
     1321 aggcggatga ggcggcgctg gttcagcgtg cccgcgccgg ggaccgcgag gctttcgcgg
     1381 ccctgttcga ccgctatcag acccccattg tgaactatct gtaccgggtg gtcggcgatt
     1441 gggagacggc caacgacctg gcgcaggact ccttcctcaa agcctaccag gcgctggccc
     1501 gcacggacac cgatctgcaa ctggccccgt ggctctaccg catcgccacc aacaccgcgc
     1561 tcgacgctct gcgccgccgc aagcgcatca cctgggtgcc gttcctggaa gagttcgagc
     1621 cgcccgcgcc gggcggcgat ccgggcacga cggtgcccaa ccaggacgcg gtgcatcgcg
     1681 cgctacaggc ggtgcccgac gattaccgca tcgccctggt gttgcatttg catcagggcc
     1741 tgtcctataa ggagatcggc gccgccctgg gcgttgcgcc caatctggtc gccgtccgcg
     1801 tcttccgcgg gcgcgagaag tttatcaagg tgtataaggc gctaactgga gaacccgacc
     1861 gatgaagtgt gcccagtgcc ggccgctggt atcgaagtat gtggatgggg aagcaagcca
     1921 ggccgagcgc cggcaggtag aagcgcacct gggcacctgc ccggcctgta gcgcgctgct
     1981 ggctcagtac cggatggcaa aggcccatgt gggcaatttg ccctattatc aacccgacgc
     2041 ccgcctgcgc gcccggctgc tggagtcgct ggacgcgcac gaccgggcgc gggccgacga
     2101 gatccccatt acgcgccggg cggtcccggc ggcgccggcc ccgcgccggg cgcacccgca
     2161 gggaggccgg ctgttcggca ccttcgccag cggtttcgcc atgttcctgg tcgcggcggc
     2221 gggcgtgctt gtctggcaga tcgccggcaa ccgcc
//
LOCUS       DASUYC010000886         2155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_172637,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000886 DASUYC010000000
VERSION     DASUYC010000886.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172637"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="VF276_14495"
     CDS             <1..884
                     /locus_tag="VF276_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX6608111.1"
                     /translation="GQTWGDAEQCERLAEEAGRQEIPISAFGVGADEDWSVSLLDKIA
                     EKSHGEADYIAQPAGMLASFRGTVQTMQNTVVKGAVLTLQLAAGVTARTIYRINPMIG
                     RVTPQQPDANTVEVTIGDVQRDRAMTVLAELVVPPRKPGQYRIVRASARSTAPNEAPV
                     EGASRDVLLNFAAGARTQGDPRVMNIVEKATAFKLQTRALQEAEIGNVEAASRNLRSA
                     ATRLLNLGETDLAQAAENEAQQLEQRGQMSSAGTKKLTFATRKLALDDVVAATTPAAP
                     PADATSGPAPGAPEPGQ"
     gene            959..>2155
                     /locus_tag="VF276_14500"
     CDS             959..>2155
                     /locus_tag="VF276_14500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608112.1"
                     /translation="MTEPLTRLRTVLVPMLNVSVAQDLLSLAAGLIAGPDRFLPHQAR
                     VLVLAVVAVPEGHPPSEGRQIARAYRAMLGYLPAAIRPPGTPPGAPDEPPGSPAIEVP
                     VDPLIKVAPTVQQGIREAVSSEDVDLLLLHWKGYSHHARQHVYGRILDDLVENPPCNM
                     LLARGSRWQTPRRLLLPLRGGPAAELALGVGLKLASRLQVGVTVLHGVPRAMPAEVGR
                     RRGDAPYLDLQRYLEHHSGRGAAVPIEQVLTLATDVPDLIAERAGADDLLILGLGDRG
                     PHQPLAGSPVLETVLADDERPVLLARAARPLDLAAYHARLSAREEIALSAEQWFVENT
                     YHRDEFADTEKWLDARNNRHGHLSVVIPTHNDAAHLGPMLVGLRRGLQPQRTPHTAGE
                     ILVIDAA"
BASE COUNT          356 a          803 c          725 g          271 t
ORIGIN      
        1 acggccagac ctggggcgac gccgagcagt gcgaacgcct ggcggaggaa gccggccggc
       61 aggagatccc gatcagcgcg ttcggcgtgg gcgccgacga agactggagc gtgagcctgc
      121 tcgacaagat cgccgagaag agccacggcg aggccgacta catcgcccag cccgcgggga
      181 tgctggcctc gttccgcggc accgtgcaga cgatgcagaa caccgtggtg aagggcgccg
      241 tgctgaccct gcaactggcc gcgggcgtaa ccgcgcgcac catctatcgc atcaacccga
      301 tgatcgggcg ggtcacgccc cagcaacccg atgccaatac cgtcgaagtc accattggcg
      361 atgtacagcg cgaccgggcc atgacggtgc tggccgaact ggtggtgccg ccgcgcaagc
      421 cgggccagta ccgcatcgtg cgcgcgtccg cccgcagcac cgcccccaac gaggcgcccg
      481 tcgaaggtgc cagccgcgat gtcttgctca atttcgccgc cggcgcccgc actcagggcg
      541 acccgcgggt gatgaacatt gtggagaagg ccaccgcctt caagctccag acccgcgcac
      601 tgcaagaagc cgagatcggc aacgtcgaag cggccagccg caatctgcgc tcggcggcga
      661 cgcgcctgct caacctgggc gagaccgacc tggcccaggc cgccgagaac gaagcccagc
      721 aactagagca gcgcggccag atgagctcgg cgggcaccaa gaaactgacc tttgccactc
      781 gcaagctggc gctggacgat gtggtcgcgg ccaccacgcc cgccgcgccc ccggcggacg
      841 cgaccagcgg cccggcgccc ggtgcgcctg agccggggca gtaacaacgc atgaaccgtg
      901 gaggcttgac aatgaggtcc gccatccagc tgcgcgccgc gaacacccga tagcgcgcat
      961 gaccgaaccg ctcacgcggc tgcgcacggt cctggtgccc atgctcaacg tcagcgtcgc
     1021 ccaggatctg ctcagcctgg cggccggcct gatcgccggc cccgaccgct ttctaccgca
     1081 tcaagcgcgc gtgctggtac tcgccgtggt cgccgtgccc gagggccacc cgcccagcga
     1141 gggccggcag atagcgcgtg cctaccgcgc catgcttggc tacctgccgg cggccatccg
     1201 gccgccgggt acgccgcccg gcgccccgga cgaaccgccc ggcagcccgg ccatcgaggt
     1261 gcccgtcgat cccctgatca aggtcgcgcc caccgtgcag caggggatcc gcgaagccgt
     1321 gagcagcgaa gacgtggatc tgctgctgtt gcactggaag ggctacagcc accacgcgcg
     1381 gcagcatgtc tacggccgga tcctcgacga cctggtggaa aacccgccct gcaacatgct
     1441 gctggcgcgg ggcagtcgct ggcagacccc gcgccggttg ttgctgcccc tgcgcggcgg
     1501 tccggccgcc gagctggcgc tgggcgtggg gctgaagctg gcgagccgcc tccaggtggg
     1561 cgtgaccgtg ctgcacggcg tgccgcgcgc catgcccgcc gaggtgggcc ggcgccgcgg
     1621 cgatgcgccg tatctcgatc tccagcgcta tctcgaacac catagcgggc gcggggccgc
     1681 cgtgcccatc gagcaggtgc tgaccctcgc caccgacgta cccgacctga tcgccgaacg
     1741 cgccggggcc gacgatctgc tgatcctggg cctgggcgac cgcgggccgc accagccgct
     1801 ggccggcagc cccgtgctcg aaactgtgct ggccgacgac gagcgtccgg tgttgctggc
     1861 gcgcgccgct cggcccctcg acctggccgc gtatcatgcc cgcctcagcg cccgcgagga
     1921 aatcgcgctc agcgccgaac agtggtttgt cgagaatacc tatcaccgcg acgagttcgc
     1981 cgatacggaa aagtggctgg acgcgcgcaa caaccggcac ggccatctca gcgtcgtcat
     2041 cccgacccac aacgatgccg cccacctggg cccgatgctg gtcggcctgc gccgggggct
     2101 gcaaccgcag cgcacgcccc acaccgccgg cgagatcctg gtcatcgacg ccgcc
//
LOCUS       DASUYC010000887         2989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1993383, whole genome shotgun sequence.
ACCESSION   DASUYC010000887 DASUYC010000000
VERSION     DASUYC010000887.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1993383"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VF276_14505"
     CDS             complement(<1..308)
                     /locus_tag="VF276_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009803716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HEX6608113.1"
                     /translation="MPDRLTVTALPAHHAGGAVDEIDIPNSAGLRIGVDVGGTFTDVV
                     CIGADGATVLAKAATTPADQSEGVLDGLAALAAELQLTLRELLEVTERLVHGTTVATN
                     A"
     gene            complement(345..1613)
                     /locus_tag="VF276_14510"
     CDS             complement(345..1613)
                     /locus_tag="VF276_14510"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HEX6608114.1"
                     /translation="MLKTKWAALGLTLALLLLIGSGMFAPRAQAQSSGPANVNVPSET
                     AAIRDLLQQSMREYRLGNYDAAYKLSRSAYLDHFENIEIPLRVMNADLTADMEFRFAA
                     LRGAMDAHQPISDVEKLNRSVRDGVDEVEGMFSGPGVLAPLFALGTSFSIIFREGLEA
                     SLVVAAVLAYLEAGNMRRLKKPVLLGVGAAIIFSAISWLLVNTIIQLAPVGREVLEAV
                     VSLVAVAMLFWVSFWLIRKMDNKRWMEFIKARAWSAMTSGSTFALAALGFTAVYREGL
                     ETALFYQVLSMNGAGVEAYVVGGFLLGVAAIGAALMVMFRAGRKLPVGRFLSIAATII
                     MVLSVAFLGNAVRELQDIGILGATSLIGIVPRLPYLLAEMTGIHPTLETILAQLALAA
                     VYATGLVAMVLKRPARRQAEGARRPASPGA"
     gene            complement(1630..2760)
                     /locus_tag="VF276_14515"
     CDS             complement(1630..2760)
                     /locus_tag="VF276_14515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608115.1"
                     /translation="MANWFRVSVPVLGLLLVLTACGEAAAPGANTQPTATVIPVATVA
                     PTSTAQATPAAGVPVDADVARTVNLLNQMRGHLMVAQDLAAAGQADAAAEQAEKASSG
                     LMDAVANTLTAKGADTALRGALDAYTAAVKQGGAAAAPAQQAALAAVDGAILAVAGPD
                     AATNTALQGQVLTGLLEKIEEEYAESIKDGKIAELEPYQSGYGILQVARLHFDALEKG
                     MPSAPVSEMTAARTALGKLSEAMPGPQPPATVVSPEAVEQQIDAAKAAIAAATGTTAG
                     TLDSAAAIAAIQADVQAALELYKAGNKDAAYEKAAGAYLDGFEKLEPDLLKADKDIVP
                     AQETDFKALRDGIKAGQSQSELEAIAARIDSGLQRAGQLLAK"
BASE COUNT          495 a         1029 c          961 g          504 t
ORIGIN      
        1 gcgttggtgg cgacggtggt gccgtgcacc aggcgttccg tgacctccag cagctcgcgc
       61 agggtgagct ggagttcggc ggcgagggcg gcaaggccgt ccagcacgcc ttccgactgg
      121 tcggcgggcg tggtcgcggc cttggcgagc accgtcgcgc catcggcgcc gatgcagacc
      181 acgtcggtga aggtgccgcc gacatccacg ccgatgcgca gcccggcgct gttggggata
      241 tcgatctcgt cgacggcgcc cccggcatgg tgcgccggaa gcgccgtaac ggtcaggcga
      301 tcaggcatag acgaccgcct ctaggtgcag attcatagcg ggtgctaggc tcccggtgag
      361 gccgggcgcc gggcgccttc ggcctggcgc cgcgccgggc gcttgagcac catcgccacc
      421 aggccggtgg cataaaccgc cgccagcgcc agttgggcca ggatggtttc gagcgtcggg
      481 tggatgccgg tcatttcggc cagcaggtag ggcaggcgcg gcacgatgcc gatgagcgag
      541 gtcgcgccca ggatgccgat gtcctgtaac tcgcgcacgg cgttgcccag gaaggccacc
      601 gacagcacca tgatgatggt ggcggcaatc gacaggaagc ggcccaccgg cagcttacgg
      661 cccgcacgga acatgaccat cagcgccgcg ccgatggccg ccacgccaag caggaacccg
      721 cccacgacat aggcttcgac gcccgcgccg ttcatcgaca gcacctggta gaacagcgcc
      781 gtctccaggc cctcgcggta cacggcggtg aagccgagcg cggccagggc aaacgtgctg
      841 ccgctggtca tggccgacca ggcgcgcgcc ttgatgaact ccatccagcg cttgttgtcc
      901 atcttgcgga tcagccagaa gctgacccag aagagcatcg ccacggcgac gagggagacc
      961 acggcctcca gcacctcgcg gcctaccggc gcaagctgga tgatcgtgtt gaccagcagc
     1021 caggagatcg cgctgaagat gatggcggcg ccgacgccca gcagcaccgg cttcttgagc
     1081 cggcgcatgt tcccggcttc gaggtaggcc agcacggcgg cgaccaccag ggaggcttcc
     1141 aggccctcgc ggaagatgat cgagaacgag gtgcccaggg cgaacaacgg cgccagcacg
     1201 cccggcccgc tgaacatgcc ctcgacctcg tccacgccgt cgcgcacgct gcgattgagc
     1261 ttctcgacat cgctgatcgg ctggtgggcg tccatggccc cgcgcagcgc cgcgaagcgg
     1321 aactccatgt cggcggtcag gtcggcgttc atgacccgca gcgggatctc gatgttctcg
     1381 aagtggtcga gataggccga gcgcgacagc ttgtaggccg cgtcatagtt gcccaggcgg
     1441 tactcgcgca tcgactgctg cagcaaatcg cgaatagccg ccgtctccga cgggacgttc
     1501 acgttggccg ggccgctgct ctgcgcctgc gcccgtgggg cgaacatccc gctaccgatc
     1561 agcagcagca gcgcgagggt caggcccagc gcagcccatt tcgtcttcag catagtgcct
     1621 ctcatgctct tacttggcga gcaactggcc cgcgcgctgc aggccgctgt cgatgcgcgc
     1681 cgcgatcgcc tctaattcgc tctgcgattg gccggccttg atcccgtcac gcaatgcctt
     1741 gaagtcggtc tcctgcgccg gcacgatatc cttgtcggcc ttcagcaagt ccggttccag
     1801 cttttcaaac ccgtccaggt aggcgcccgc cgctttctcg taggcggcgt ctttattccc
     1861 cgccttatac aactccagcg cggcctgcac atccgcctgg atggccgcga tagcggcggc
     1921 gctgtccagc gtaccggcag tcgtcccggt cgccgcagcg atggccgcct tggcggcgtc
     1981 gatctgctgc tccacggcct cgggcgacac gaccgtggcc ggcggctgcg ggccgggcat
     2041 ggcctcgctc agcttaccca gcgccgtcct tgcggcggtc atttcgctca ccggcgcgga
     2101 aggcatgccc ttctccaggg cgtcgaagtg caggcgcgcg acctgcaaga tgccgtagcc
     2161 gctctggtac ggctccagtt ccgcaatctt gccgtccttg atcgattcgg catattcctc
     2221 ctcgatcttc tccagcaggc cggtcaagac ctggccctgc aacgcggtat tcgttgccgc
     2281 gtcggggccg gccacggcaa ggatcgcgcc atcgacggca gcaagtgccg cctgctgcgc
     2341 gggggcggcg gccgcgccgc cctgcttcac ggcggcggta taggcgtcga gcgcaccgcg
     2401 caacgcggta tcggcgccct tagccgtcag cgtgttcgcc acggcgtcca tcaagccgct
     2461 acttgccttc tcggcctgct cggcggcggc atcggcctgg cccgccgcgg ccagatcctg
     2521 ggccaccatc aggtggccgc gcatctggtt caataggttc accgtgcgag ccacatcggc
     2581 atcaacgggc acgcccgctg ccggggtcgc ctgggccgta ctcgtgggcg ccaccgtggc
     2641 gaccgggatc actgtcgccg tcggttgagt gttggcgccg ggcgcagcag cctcgccgca
     2701 ggcagtcaga accaataaca gtcccaggac cgggacactc acgcgaaacc agttcgccat
     2761 cacagaaatc gtaactcctc ttgcagcctt acagtaatgt cccaggtccg gtgaagcata
     2821 ttcgtatatg ctaatatctg gcatgagcct atacgaacgt aaaatactta cccaggctaa
     2881 tgcagtatag cagattgcca aatcgctgtc aacgttttcg cacctccagt tgtgtaaagt
     2941 cccggtaaaa tctttcccgc cgcctctccc acacgccgta gtagctgca
//
LOCUS       DASUYC010000888         3592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1187966, whole genome shotgun sequence.
ACCESSION   DASUYC010000888 DASUYC010000000
VERSION     DASUYC010000888.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187966"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VF276_14520"
     CDS             <1..553
                     /locus_tag="VF276_14520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608116.1"
                     /translation="STVGVVPRIRRFTHEDLAVNLAISLHAPNDALRSRLVPLNEKYP
                     IADLMAACREYIDVTKRRLTFEYALIAGENDSLECAEELGRLLQGMLVHVNLIPLNPV
                     PGTGLQATPREHVRAFAAAVERHGVPCTVRIERGQDIAAACGQLKVEHEPNSRRRSLN
                     PTVVDQEAAPASAPALVIPAHEI"
     gene            558..1763
                     /gene="larC"
                     /locus_tag="VF276_14525"
     CDS             558..1763
                     /gene="larC"
                     /locus_tag="VF276_14525"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HEX6608117.1"
                     /translation="MLAFFDPVSGVSGDMALGALLDAGLPLAALEAELARLHVPGFRL
                     DASRAEQHGLTGTRVRVLLDDTPQPERHLRDIAAILDGSTLAPAVRERALAVFQRLAV
                     AEARVHGTTPDQVHFHEVGALDSIVDIVGVVAGLALLGVDECYCGPLPLPLRGGIGRS
                     AHGPIPLPGPATLEILSAVNAPFISRDTDRELVTPTGAALVAELCRFEQPPLRLQRVG
                     VGFGQRTLPWPNVLRLLLCESVAAPSPAAGLEQDRVVLLETNIDDMNPQIYGYLLDRL
                     LGAGALDAWLTPIQMKKGRPATLVSVLTPPAAADALSALLLRETSTLGVRRRELDRLK
                     APRRMLTVVTSAGPIRVKARLLDGAWTAQPEYDDCLAAAQRTGRPLREILDEAATEAR
                     RLLSADAQA"
     gene            complement(2046..2774)
                     /locus_tag="VF276_14530"
     CDS             complement(2046..2774)
                     /locus_tag="VF276_14530"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX6608118.1"
                     /translation="MIPLDLIREVTQVAGLAPGARVLEIGAGTGQLTAALRALRLRVV
                     ALEPGAHMRNLLRARFPSDANLNVQAGFFEDYRVGGAFDAIAAANAFHWLDPAVSYHK
                     AADLLVGNGALVLIWNYAILDAPVQRLLNTQVFAGAYPDFVSDPDTFLAEVEANAVAG
                     RAELAASGRFAEPWWQFTVEHFTLDVARYTGLLISYANGAARDVPARRELADRIHAAL
                     AAAGIEQLAMINYLYALVARKADA"
     gene            complement(2846..>3592)
                     /locus_tag="VF276_14535"
     CDS             complement(2846..>3592)
                     /locus_tag="VF276_14535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608119.1"
                     /translation="GHWHVLNARVGAWLARRQYPHADIALLTGGFSYSEGDKYAGDLF
                     PRWTTGTATVALPDIAAYPLTLTMRLADHRPPTLPRADLALSLAGQPVPADRQPVPTS
                     PVESVLTATIARPTVPAWDLMNLALISPTWNPKQAGAGARNEDLGLLVESLRLQRADG
                     RPLRLVEALPVQPMYPAPRWYYDTQSPHLVDVWVWYLNAAHLPRRATVVLAGLVVLFG
                     GATLAWGAWRWRRTMSGAALPRQPTDQEIT"
BASE COUNT          564 a         1271 c         1211 g          546 t
ORIGIN      
        1 ctcaacggtg ggtgtggtgc cgcgcatccg gcgcttcacc cacgaggacc tcgccgtcaa
       61 cctggcgatc tcgctgcacg cgccgaacga cgccctgcgc agccggctcg tgccgctgaa
      121 cgaaaagtac cccatcgccg acctgatggc cgcctgccgc gagtacatcg acgtgaccaa
      181 gcgccgcctg accttcgaat acgcgctcat cgccggcgag aacgacagcc tggagtgcgc
      241 cgaggagttg ggccgcctgc tgcaagggat gctggtgcat gtcaacctga tcccgttgaa
      301 cccggtgccg ggcaccggcc tgcaagccac cccgcgcgag cacgtgcgtg ccttcgcggc
      361 ggcggtcgag cggcacggcg tcccctgcac cgtgcgcatc gagcgcggcc aggatatcgc
      421 cgccgcctgc ggtcagttga aggtggagca cgagccgaac agccgccggc gcagcctcaa
      481 tcccacggtc gtcgatcagg aagccgcccc ggcgtccgcg ccggccctcg tcatccccgc
      541 ccacgaaatc taagccgatg ctggcgtttt tcgacccggt ctccggcgtc agcggggaca
      601 tggccctggg cgcgctgctc gacgccgggt tgcccctggc cgcgctcgag gccgaactgg
      661 cccggctgca cgtgcccggc ttccggctgg acgcgagccg cgctgagcag cacggcctga
      721 ccggcacccg tgtccgtgtg ctgctggatg atacgcctca acccgaacgc cacctgcgcg
      781 acatcgccgc catcctggac ggcagcacgc tggcccccgc ggtccgcgag cgcgcgctgg
      841 ccgtgttcca gcgcctggcc gtggccgagg cgcgcgtcca cggcaccacg cccgaccagg
      901 tccacttcca tgaggtcggc gcgctcgatt cgattgtcga tatcgtgggc gtcgtcgcgg
      961 ggctggccct gctgggcgtg gacgagtgct actgcgggcc gctgcctctg cccctgcgcg
     1021 gcggcatcgg gcgcagcgcg cacggtccca tccccctgcc cggcccggcc accctggaga
     1081 tcctgagcgc ggttaacgcg cccttcatca gccgcgacac cgaccgcgaa ctggtgacgc
     1141 cgaccggggc tgcgctggtc gccgagcttt gccggttcga gcagccgccc ttgcgcttgc
     1201 agcgggtggg cgtgggcttc ggccagcgca ccctgccctg gcccaatgtg ctgcgcctgc
     1261 tgttgtgcga atcagtggcc gcgccctctc ctgccgccgg gctggagcag gaccgggtgg
     1321 tgctgctgga aaccaacatc gacgacatga acccgcagat ctacggctac ctgctggacc
     1381 ggctgctggg cgccggggcg ctggacgcct ggctcacgcc gatccagatg aagaagggcc
     1441 gcccggccac gctggtgagc gtgctcacac cgcccgccgc cgccgatgcc ttgagcgcgc
     1501 tgctgctgcg cgagaccagc acgctcggcg tgcgccgccg cgaactggac cggctgaaag
     1561 cgccccgccg catgctcacc gttgtgacca gcgccggccc gatccgtgtc aaggcccgcc
     1621 tgctggacgg cgcctggacg gcccagcccg aatacgacga ctgcctggcc gccgctcagc
     1681 gcacgggccg ccccctgcgc gagatcctgg acgaagcggc tacggaagcg cgacggctgc
     1741 tcagcgcgga cgcgcaggcg tagaaagcga caaacccggc gcaagcgccg ggtctgccat
     1801 tttcctcgcg tcacgaacat accgtttgcg aggcagttac ctgcccgcgc gagtatctcc
     1861 ctgaacagcg aggttgtctg ctgctttgta catcagacat ccctcctccc cttacggtag
     1921 acttgggacc acgacttacg tagccgcagc cactatttaa tatagcaagc ggcgtgccag
     1981 cccccgtctc caatagggat cgcgttccct gcttcccaga tcaatgtgcg caatgcgcgc
     2041 tcagcttaag cgtctgcctt acgcgcaaca agagcataca ggtagttgat catcgccaac
     2101 tgctcgattc cggcggcggc gagcgcggcg tgtatccggt cggccaactc ccggcgcgcc
     2161 ggcacgtccc gcgcggcgcc gttggcgtag gagataagca agccagtgta gcgagccaca
     2221 tcgagcgtga agtgctcaac ggtgaattgc caccagggtt cggcaaagcg cccgctcgcc
     2281 gccagctcgg cccggcccgc aacggcgttg gcctcgactt cggccaggaa ggtgtcggga
     2341 tcgctgacga agtccgggta ggcgccggca aacacctgcg tgttgaggag ccgctggact
     2401 ggggcatcga gaatggcata gttccatatc agcacaagtg ccccgttccc cacgaggaga
     2461 tcagcggctt tgtgatagct gacggcggga tccagccagt ggaaggcgtt ggctgcggca
     2521 atcgcgtcaa aagccccacc gacccgataa tcctcgaaaa accccgcctg cacgttcagg
     2581 ttcgcgtcgg acggaaagcg cgcacgcaac aggttccgca tatgcgctcc cggttcgagg
     2641 gcgaccacgc ggagccgcag ggcgcgcaaa gcggcggtga gttgcccggt gccggcgccg
     2701 atctccagca cccgcgcccc tggcgccagg ccggccacct gtgtcacctc acgaatgagg
     2761 tccagcggaa tcataggccg agcctcgtca tagatctccg ccactgtgtc aaagacgatc
     2821 cggttcgcgc ggttcgtatt agccttcatg taatctcctg atctgttggt tgccgtggca
     2881 gcgcggcccc gctcattgtc ctccgccagc gccaggcgcc ccaggccagc gtcgcgccgc
     2941 cgaacagcac cacgagcccg gcaagcacga ccgtggcccg gcgcggcagg tgggcggcgt
     3001 tgagatacca gacccagaca tcgacgagat gcgggctttg cgtgtcatag taccagcgcg
     3061 gcgccgggta catcggttgc accggcaggg cttcgaccag gcgcaagggc cgcccgtcgg
     3121 cgcgttgcag gcgcagcgac tcgaccagca ggcccaggtc ctcgttgcgc gcgcccgccc
     3181 ccgcctgctt gggattccag gtcgggctga tcagggccag gttcatcagg tcccaggcgg
     3241 gcacggtggg gcgggcaatc gtcgccgtca gtaccgactc gacgggacta gtgggcaccg
     3301 gctgccggtc ggcgggcacc ggttgcccgg ccagactcag cgcgaggtcg gcgcgcggca
     3361 gagtcggggg ccggtggtcg gccaggcgca tggttaacgt gagcgggtag gcggcgatgt
     3421 cgggcagggc gacggtggcg gtgccggtgg tccagcgcgg gaacaggtcg cccgcgtatt
     3481 tatcgccctc gctgtaggag aagccgccgg tgagcaaggc gatatcggcg tgcgggtact
     3541 gccgccgggc cagccaggcg cccacgcggg cgttcagcac gtgccagtgg cc
//
LOCUS       DASUYC010000889         3026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1994065, whole genome shotgun sequence.
ACCESSION   DASUYC010000889 DASUYC010000000
VERSION     DASUYC010000889.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1994065"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..523
                     /locus_tag="VF276_14540"
     CDS             185..523
                     /locus_tag="VF276_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608120.1"
                     /translation="MPRAPGWPGRPGAAYPHSGQTRQADGNAPPLVQSPIERQRSREV
                     CLRHTVVSLVSLRGRNKAETAQGYGLPEAIALGWHGRQTCFKMRLGLRIVLLREGDQT
                     QPDDWLGRHQ"
     gene            complement(555..713)
                     /locus_tag="VF276_14545"
     CDS             complement(555..713)
                     /locus_tag="VF276_14545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608121.1"
                     /translation="MDRTDGVDGFQQELADGIVIADLLGCPAHCLQPDGPVMEILGID
                     QQEAGREM"
     gene            1466..1801
                     /locus_tag="VF276_14550"
     CDS             1466..1801
                     /locus_tag="VF276_14550"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX6608122.1"
                     /translation="MRDHPAPGAPPRLGLEQQVQLAALLLEGAEACGFRGDVWTCRRV
                     AQLIKEQFAVSYHPAHVSRILAHLGWTPQKPVIRASQRDEAVIAAWYSERWPALKKSA
                     GATDESSSG"
     gene            1834..2232
                     /locus_tag="VF276_14555"
     CDS             1834..2232
                     /locus_tag="VF276_14555"
                     /inference="COORDINATES: protein motif:HMM:NF024751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX6608123.1"
                     /translation="MRSYAPCGQTPVLRVPLTRDHLSVISGITGAGQLLGAVQARAFK
                     GRDAVRFLQHRQCQLSGRLPIIWDGAPIHHDSKVTSFVAETAGQICLEPLPGYAPDLN
                     PAEGGWDQLKYVEMKNVTSPDQAELRHDCV"
     gene            2449..>3026
                     /locus_tag="VF276_14560"
     CDS             2449..>3026
                     /locus_tag="VF276_14560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608124.1"
                     /translation="MSIEEVAHRLALIWSPSMPLSSVRPISATTLSHYIRLHQCERYL
                     RFRLHQPSARSLFDAYKALGVRAEQPLTPLLREAGRRFEERVMALLPPPVSDLRGSDA
                     AATRRILAALGPNQPHYLVQAPLAAQIGDWRCVGCADIIRATRRPDGALDLSIADIKA
                     SQHDRVEYRLQVTFYARLIGAMLVDLGIPLGIV"
BASE COUNT          571 a         1030 c          874 g          551 t
ORIGIN      
        1 tccagggcca gggcgatcgc ttcctccagc ggtaggcgct gccccgccgc ccattcagcg
       61 gcgaaccggg tcccgccgag gccagcccgt gccgcagctt ggttctgctc gtagaagatc
      121 cggtcctggg gatccagcac cgcgccggct tcctgcagaa cggccgcggc ggcgcccagt
      181 agctgtgccg cgcgccccag gatggccggg tcgccccggc gcggcctacc cgcactccgg
      241 ccagacccgc caggcagacg gcaatgcccc gccgctggtc cagagcccga ttgagcgcca
      301 gcgctctcgc gaagtgtgcc tgcgccacac ggtagtctcc ttagtctcct tgcgcggtcg
      361 caataaggcc gagactgccc agggctacgg cctccccgaa gcgatcgccc tgggctggca
      421 tggccgccag acctgcttca agatgcgtct gggcctgcgc atagtgctgc tgcgcgaagg
      481 cgaccagacc caaccagacg attggctcgg ccgtcaccaa tgaacccgct gaacagcttg
      541 cggacagcta caccttacat ttcgcggcca gcttcctgct ggtcgatccc gagtatttcc
      601 atgaccgggc cgtcgggctg gaggcaatgg gccgggcaac caaggagatc agcgatcact
      661 ataccatcag ccaactcctg ctgaaaccca tcgaccccat cagtgcgatc caagagccga
      721 cactccacca ggagcgcgta acgcgtgcct tcgccgccta ccagcaagct gagcccgagc
      781 gcgcccggca cctagtcaag cagtatgctg tcccgacata caacattagg gtgtaacact
      841 cgtttcgaca ggatccgtag ggcgccacac caccgtagtt aatcgttaac cacagcaaaa
      901 ttgcgcgggt ccatggtgaa tctcggggat ttcccattcc caaggtacgc catagacccg
      961 cgttcgctat tgtgcccaat cgggataccc gcccaacaac gcaatcaagc ctccggctcc
     1021 tcctccatca acccccaacg cgatggaccc ttcaacctaa tcagccaccg ctggtagtgt
     1081 atccgcgcca cctcgacccc acaccgccca taccaatgct cgctgagctg ccgcagcggc
     1141 cgcaccaggc acggccacgc gaaagccgcc cggtacaccc ggagcccatt cgtcgggtgt
     1201 cccctcagat cagcgatcct cgatcgccta tctactgcgt gttgcattga tctctaataa
     1261 tcgtgggccg agaaatgctc tgttcacaga tcatgagaac cacaaagcac aacccgtcgg
     1321 tcagttggcg tgcggggcgc cgccagcgtg cttgggaact gcacaaacaa ggctggaccc
     1381 aacagcagat cgcccaagcg ctcctgttag ccaaggggcg gtcagccagt ggctcaagcg
     1441 cgccgcttgg cacgggccgc agtctttgcg tgaccaccca gctcccggcg ccccgccccg
     1501 gcttggtctt gagcaacagg tgcaactggc cgccttgctg ctcgaaggtg ccgaggcttg
     1561 cggctttcgc ggtgatgttt ggacgtgccg ccgcgtcgcc caactgatca aggagcagtt
     1621 tgccgtgagt taccatccgg cgcatgtcag tcgtatcctt gcccacctag gctggacgcc
     1681 ccaaaaaccg gtcatccgcg caagccagcg tgatgaagcg gttattgccg cttggtatag
     1741 cgaacgctgg ccggccctta aaaaaagtgc cggcgcgacg gacgaatcat cgtctgggta
     1801 gacgaggccg gtttctacct gttggcagga gtggtgcgca gttacgcccc gtgtggacag
     1861 acgccagtgt tgcgggtacc gctgacgcgc gatcatctct cggtgatcag cgggatcacc
     1921 ggggcaggcc aattgctggg ggcggtgcaa gcgcgagcat tcaaaggccg ggatgcggtg
     1981 cgcttcctgc aacaccggca atgccagttg agcggcagac tgccgatcat ttgggatggc
     2041 gcacccatcc atcacgacag caaagtcacc agcttcgtgg cggaaaccgc ggggcagatc
     2101 tgcttggaac cgttgcccgg ctatgcgccg gacttgaacc ccgccgaagg gggctgggat
     2161 cagctgaagt atgtcgagat gaaaaatgtg acctcccctg atcaggcgga actccgccac
     2221 gactgcgttt agcgattgcc cggctgcggc acaagccgga gctgattcgc cactttatca
     2281 agcactaggg ttattagatc tttatgcaag gcccagtaga aacttcccag ccctcctgca
     2341 actcgcgatc ttaggggaac ttccccgtgc gccgaccttg caaatttacc catataaggg
     2401 tagaataata cagcctcagc aattctgccc ccggtcggaa tccgcttggt gtcaatcgaa
     2461 gaggtggccc accgtcttgc cctgatctgg agcccgtcca tgcctttgtc ctccgtgcgc
     2521 cccatttccg ccaccaccct cagccactat atccggctgc atcagtgtga gcgctatttg
     2581 cgctttcgtc tccatcagcc gagcgctcgt tcattgttcg atgcctataa agcgttgggc
     2641 gtgcgggccg agcagccgct gaccccgctc ctgcgcgaag ccgggcggcg ctttgaggag
     2701 cgtgtcatgg cattgctgcc gccgccggtg agcgatttac gcggatccga tgccgccgcc
     2761 acccgccgga tcctagcagc gctcgggccc aaccagcccc actaccttgt ccaggcccca
     2821 ctggccgcgc agatcggtga ctggcgctgt gtcggctgtg ccgatattat ccgggccacc
     2881 cgccgccccg acggcgcgct ggacctgtcg atcgccgaca tcaaggcatc ccaacacgac
     2941 cgggtggaat accggctcca ggttaccttc tacgcacggt tgatcggggc catgctggtc
     3001 gacctgggta tcccgctggg catcgt
//
LOCUS       DASUYC010000890         2777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_607522,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000890 DASUYC010000000
VERSION     DASUYC010000890.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607522"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            417..>2777
                     /locus_tag="VF276_14565"
     CDS             417..>2777
                     /locus_tag="VF276_14565"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding domain-containing protein"
                     /protein_id="HEX6608125.1"
                     /translation="MYRRDRSGSSRSHDRDGRDSRSRSASAVAAPRDVRDSREAERAG
                     LHWVRVDMHLHTPASSDYRDPDATYLKILQKAEERGLDIIALTDHNSVQGYAQMLREI
                     ETLELLERLGRLNEQEAHDLAEYRRLRETLLVLPGFEFTATFGFHILGIFPPGTSLRK
                     LELLLLKLDVPEQKMLAGAPDAGPTSDVIEAYEAIAEAGGLPIAAHANSSNGVAMQGF
                     NFGGQTKIAYTQHPALACLEVTDLEQTGRRTTESFFNGSKPEYPRRMHCIQGSDAHSL
                     EKEPGEGANKRLGVGDRCTELLLPDATFEALRDLFASSDFDRIRPYRPGQVSTYDFVR
                     QARSQGPSIVQAFHERATSKTSRTRPILKDVAAFANTSGGTIYVGVSPDENVPVRGID
                     NIDDTTNILRFDIQRSIVPPLDVTFATHMSEGRPVLLLQVPRGEDRPYMFEPAGQIYI
                     RQESDSVVATRDDIVRLVLESHGERVTAELAAAALSVPTSDSIATADALEQADELSGD
                     MVEAAIEGASVPIDVAHRRKRRRRRGSEGRPGAETPVEAELDSVPAWDTTPAWEGPED
                     TQPAPTEADVEAAELAALEDVAGATAAAWPAGWTTPEEEPAPVEAPPEPARGRRRRGR
                     GAAQPAAEPVAAGPVAEPVAEAVEVVAEEVPAEPEAAPVLEPEAPRPRRKGHLYQVYR
                     SELGEPEVGNALRERRAGLYVDKAGELLTAEVRAANPDGMFEMERNQIWLPDGTAPVL
                     PEGRSALRRSKTALNDSILNARSGPGGRFQSAAELAPPPPAPEPEPE"
BASE COUNT          457 a         1009 c          947 g          364 t
ORIGIN      
        1 gtgccggcgg atcagccgga tggcaccgcg gcccccggcg attccgtgga gtcgcaccag
       61 gaggtgccgc cgcaggcggc ccaacccaac ctccagccct cggcctgaca cgggagcgcc
      121 cggcgccacc agtagggcgc cgggcggaac cccgttccgg ttattcgctc ccggtgcacc
      181 ccgccgggct agcggcctcg cagcaagtat acgattgaag cggattatca gcgcgaaccg
      241 aatctaacgg cacaaaggcc ggtcgagcgc gggcctttgt gaagctgtat ggtgcccgcc
      301 gggtgatccc ggcattgtga cggcatccct ttgcttcccc gcccgcggtc gtgacccgac
      361 cggccctgcc gcctgcgcgc cctccgcccc acccttaagg aattacacga ttgactatgt
      421 atagacgaga tcgctccggt tcgagccgga gccacgaccg cgatggccgc gatagccggt
      481 cccggtcggc gagcgccgtg gccgcgccgc gcgatgtgcg cgacagccgt gaagccgagc
      541 gcgcggggct gcactgggtg cgagttgata tgcacctgca cacccccgcc tcctccgact
      601 accgcgaccc ggacgccacc tacctcaaga ttttacagaa ggccgaggag cgcggcctcg
      661 atattatcgc tctgaccgac cacaacagcg tgcagggcta tgcccagatg ctgcgcgaga
      721 tcgagacgct cgaactgctg gagcgcctgg gccgcctcaa cgagcaggaa gcccacgacc
      781 tggccgagta tcgccgcctg cgcgaaaccc tgctcgtgct gccgggcttc gagttcaccg
      841 ccaccttcgg cttccacatc ctgggcatct tcccgcccgg cacctcgctg cgcaagctcg
      901 aattgctgct gctcaagctc gacgtgcccg agcagaagat gctggccggc gcgcccgatg
      961 ccggccccac cagcgacgtg atcgaggcct atgaagccat cgccgaggcc ggcgggctgc
     1021 cgattgccgc gcacgccaac tcctccaacg gggtggcgat gcagggcttc aatttcggcg
     1081 ggcagaccaa gatcgcctac acccagcatc cagccctggc ctgtctcgaa gtgaccgacc
     1141 tggagcagac cgggcggcgc accaccgagt cgttcttcaa cggctcgaag cccgaatatc
     1201 cgcggcggat gcactgcatc cagggcagcg atgcccacag cctggagaag gaaccgggcg
     1261 agggcgcgaa caagcgcctc ggcgtgggcg accgctgcac cgaactgctg ctgcccgacg
     1321 ccacgtttga agccttgcgc gacctgttcg ccagcagcga cttcgaccgc atccggccct
     1381 accgccccgg ccaggtgtcc acctacgatt tcgtgcgcca ggcgcgctcg caagggccgt
     1441 cgattgtgca ggcgttccac gagcgggcca ccagcaagac cagccgcacc cggcccatcc
     1501 tcaaggatgt ggccgccttt gccaacacca gcggcggcac catctatgtc ggggtcagcc
     1561 cggacgagaa tgtgcccgtg cgcggcatcg acaacatcga cgacacgacc aacatcctgc
     1621 gcttcgacat ccagcgcagc attgtgccgc cgctcgacgt gaccttcgcc acccacatga
     1681 gcgagggccg tcccgtgctg ctgttgcagg tgccgcgcgg cgaagaccgg ccctacatgt
     1741 ttgagccggc gggccagatc tacatccgcc aggagtccga ctccgtcgtg gcgacgcgcg
     1801 acgacatcgt gcgtctggta ctggagtcgc acggcgagcg cgtgacggcg gaactggcgg
     1861 cggcggccct ctcggtgccg acctccgaca gcatcgccac cgccgatgcg ctggagcagg
     1921 ccgacgaact gagcggcgac atggtcgaag cggccatcga gggcgcgtcg gtgcccatcg
     1981 acgtggccca tcgccgcaag cggcgccgcc gccggggtag cgagggccgt ccgggtgccg
     2041 agacgccggt ggaagccgaa ctcgattcgg tgcccgcctg ggacaccacc ccggcctggg
     2101 aaggccccga agatacccag ccggcgccca ccgaggccga cgtggaagcc gctgagctgg
     2161 cggcgctgga ggacgtggcc ggggccaccg ccgcggcctg gcccgccgga tggaccacgc
     2221 cggaagaaga accggccccg gtggaagccc cgcccgagcc ggcgcggggt cggcgccgcc
     2281 ggggccgtgg cgccgcgcag cctgccgccg aaccggtggc tgcggggccg gtggccgagc
     2341 cagttgcgga ggccgtcgag gtcgtggcgg aggaggttcc cgcggagccg gaagccgccc
     2401 cggtgctgga gccggaggcg ccccgcccgc gccgcaaggg ccacctgtac caggtgtacc
     2461 gcagtgaact gggcgagccc gaggtgggca acgccctgcg cgagcgccgc gccggactct
     2521 acgtcgataa ggcgggcgag ttgctgacgg cggaggtccg cgccgccaat cccgacggca
     2581 tgttcgagat ggagcgcaac cagatctggc tgccggacgg gaccgcgccg gtcttgcccg
     2641 aagggcggag cgcgctccgc cgctcgaaga cggcgctgaa cgacagcatc ttgaacgccc
     2701 gcagcggacc gggcggccgg ttccagtcgg cggcggagct tgcgcccccg ccgcccgcgc
     2761 cggaaccgga gccggag
//
LOCUS       DASUYC010000891         4084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1586616, whole genome shotgun sequence.
ACCESSION   DASUYC010000891 DASUYC010000000
VERSION     DASUYC010000891.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1586616"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..328)
                     /locus_tag="VF276_14570"
     CDS             complement(56..328)
                     /locus_tag="VF276_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX6608126.1"
                     /translation="MTTDEIEAQVREFLVSNFLFDPSAALAAEDSLLENGVVDSTGML
                     EVIQFLEATFDIQVDDMEVLPENLDSVRNLIRFIVRKRAAEAADAA"
     gene            complement(537..1574)
                     /locus_tag="VF276_14575"
     CDS             complement(537..1574)
                     /locus_tag="VF276_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608127.1"
                     /translation="MTTTEQRICTRCVLPETFPGIKFDADGVCNYCHRALTEEKAAAH
                     RESARLKFEQILNDVRGKGTYDVLVGWSGGKDSTYILARMVREFNCRVLAFTFDNGFV
                     SKQAFRNMEHISDTLGVDHLIYKARLDVLKTVFNASAVGDELYPKSALGRASNICTSC
                     MGLAKGIALRVCIEKNIPVTAFGWTPGQVPLAAGIFKSNPAMIRKMQELSVAPLRRLV
                     GDAVRPYFLEEEHYERPERFPYNIAPFAFMEYDEATIYDYVASLGWVRPKDTDANSTN
                     CLLNSYGIHVHLEQSGFHPYVAELAGMVREGRMTRDEALARINLMPNPNVVNFVTDRL
                     GVPAIVPAGPR"
     gene            complement(1615..3624)
                     /gene="asnB"
                     /locus_tag="VF276_14580"
     CDS             complement(1615..3624)
                     /gene="asnB"
                     /locus_tag="VF276_14580"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX6608128.1"
                     /translation="MCGIAGIFNRDPQQPVAPAQINALLGAIHHRGPETAGMVLDGPV
                     GLGHDRLSIIDLAGGLQPIPNEDGSIWIICNGEVFNYVELRAALIARGHQFRTGSDSE
                     VIVHLYEDHGPACVHDLIGQFAFALWDRRRGTLLLARDRLGVRPLFYAEVPGALLFAS
                     EIKALLADPRVPRALDLEALDQVFTYWAPLPGRTAFRGVEEVPPGHYLLANRHGCTLR
                     RYWTLDFSDEPAGPGLGARHYAERLLELLIDATRLRLRADVPVGAYLSGGLDSSAIAA
                     VVRRYTGNPLKTFSIAFSDSRFDEREHQQRMADVLGTEHISLVCDDADIGAVFPDVIR
                     HAEMPLLRTAPAPMYLLARQVRAHGMKVVLTGEGSDESFGGYDIFKEARIRRFWAARP
                     ESRVRPLLLQTLYADIASMNGVSAAYRTAFFKQDLDSVDDLCYSHRLRWRNTARLKRL
                     FSASTRAALADYAGEDTLALALDGMGRAWHPLSQAQFIESRIFLSQYLLSAQGDRMAM
                     AHAVEGRFPFLDHRLVEFAATIPAEFRLRGLDEKYILKRALADVLPAAVLARTKRPYR
                     APIRNVFFGPQAPAYVRDMLSPEAVAQAGIFDPAAVGLLVKKCQAAPDLGEVDSMGLV
                     GVLSTQLLYHQFIAPGPAPTMPVSAVILDPATPATPALALAGPSS"
BASE COUNT          714 a         1301 c         1362 g          707 t
ORIGIN      
        1 gccgctgctg agcggtggct tcgaggaacc gctgcgccgg catggcgatg ccccgctagg
       61 cggcgtccgc cgcctcggcg gcacgcttgc gaacgatgaa gcggatcagg ttgcgcaccg
      121 aatccaggtt ctccggcagc acttccatgt cgtctacctg gatgtcgaag gtggcttcga
      181 ggaactggat cacttccagc atgccggtcg aatcgacgac gccgttctcc agcagggagt
      241 cttcggcggc cagcgccgcg ctcgggtcaa agaggaaatt gctgaccagg aactcgcgca
      301 cctgggcttc gatctcgtcg gtggtcattc cgtgtcgttc tccttacatc acatcggtca
      361 ttctgagcgc agcgaagaat ctccgtctta tccccacgcg ccgagactct tcgctccgct
      421 cagagtgaca atactcatcg gtcattctga gcgcagcgaa gaatctccgt ctcgttaggg
      481 gaaacgacga gactcttcac tgcgctcaga gtgacaattc atggctagca gactcatcag
      541 cgcggcccgg cggggacaat cgcgggcacg ccgaggcggt cggtgacaaa gtttaccaca
      601 ttcgggttgg gcatcaggtt gatgcgggcc agggcttcgt cgcgggtcat gcgaccctcg
      661 cggaccatgc cggccagttc ggccacgtag gggtggaagc ccgactgctc caggtggacg
      721 tggatgccgt agctgttgag caggcagttg gtcgagttgg cgtcggtatc cttcgggcgc
      781 acccagccca gcgaggccac gtagtcgtag atcgtcgcct cgtcgtactc catgaaagcg
      841 aagggcgcga tgttatacgg gaaccgttcg ggccgctcgt agtgctcttc ttcgaggaag
      901 tacggacgca cggcgtcgcc cacgaggcgg cgcagcggcg cgacgctcag ttcctgcatc
      961 ttgcggatca tggccgggtt gctcttgaag atgcccgccg ccagcggcac ctgccccggc
     1021 gtccagccga acgccgtcac cgggatgttc ttctcgatgc agacgcgcag cgcgataccc
     1081 ttggcgaggc ccatgcacga tgtacagata ttgctggccc gccccaatgc cgacttcggg
     1141 tagagttcgt cgcccaccgc cgaggcgttg aacaccgtct tgagcacgtc gaggcgcgcc
     1201 ttgtagatca ggtggtcgac gcccagggtg tcggagatat gctccatatt gcggaaggct
     1261 tgcttggaga caaagccgtt gtcgaaggtg aaggccagca cccggcagtt gaactcgcgg
     1321 accatgcgcg ccaggatata ggtgctgtcc ttgcccccgc tccagccgac caggacatca
     1381 taggtgccct tgccgcgcac gtcgttgagg atctgctcga acttgaggcg ggcgctctcg
     1441 cggtgcgccg cggccttctc ctcggtcagc gcgcggtggc agtaattgca gacgccgtcg
     1501 gcgtcgaact tgatgcctgg aaaggtctcc ggcagcacgc agcgcgtgca gatacgttgc
     1561 tcggtggtag tcacgaagaa tccttctttc gggtgagcgg tgggtggcag ccgctcagct
     1621 agacggcccg gccagcgcca acgccggcgt tgccggcgtc gccgggtcga ggatcacggc
     1681 ggaaaccggc atcgtggggg ccggccccgg cgcgataaac tggtggtaga gcagttgcgt
     1741 ggacaacacg cccaccaggc ccatgctgtc cacttcgccc aggtcgggcg cggcctggca
     1801 ctttttcacc agcagaccca ccgccgccgg gtcgaagatc ccggcctgcg cgacggcctc
     1861 gggcgacagc atgtcccgga cgtaggccgg ggcctgcggg ccgaagaaga cgttgcggat
     1921 cggggcgcga taggggcgct tggtgcgcgc cagcacggcg gcgggcagca catcggccag
     1981 ggcgcgcttc aagatatatt tttcgtccag cccgcgcagc cggaactcgg cggggatggt
     2041 cgccgcgaac tcgaccagcc ggtggtcgag gaacgggaag cgcccctcga cggcgtgggc
     2101 catcgccatg cggtcgccct gcgcgctcag cagatactgc gacaggaaga tgcggctctc
     2161 gatgaactgc gcctgcgaca gcgggtgcca ggcgcggccc atgccgtcga gcgccagggc
     2221 cagcgtatcc tcgccggcat aatcggccag cgcggcgcgg gtcgaggccg agaacagccg
     2281 cttgaggcgg gccgtgttgc gccagcgcag ccggtggcta tagcacaggt cgtcgaccga
     2341 gtccaggtct tgcttgaaga aggctgtccg gtaggctgct gacacgccgt tcatggacgc
     2401 aatatcggcg tagagcgttt gcagcagcag cgggcgcacg cgcgactccg ggcgggccgc
     2461 ccagaagcgg cggatgcgcg cctccttgaa gatgtcgtag ccgccgaagc tctcgtcgga
     2521 cccctcgccg gtcagcacga ccttcatgcc gtgcgcccgg acctgccgcg ccagcaggta
     2581 catgggcgcg ggggccgtgc gcagcagcgg catctcggca tggcggatca cgtcggggaa
     2641 gaccgcgccg atatcggcgt cgtcgcagac caggctgatg tgttcggtgc ccagcacatc
     2701 ggccatgcgc tgctggtgct cgcgttcgtc aaagcggctg tcgctgaagg cgatggagaa
     2761 ggtcttgagc gggttgccgg tatagcggcg gaccacggcg gcgatggccg acgagtctag
     2821 cccgccgctc aggtaggcgc cgaccggcac gtcggcgcgc aggcgtagcc gggtcgcgtc
     2881 gatcagcagt tccagcagcc gctcggcgta gtggcgcgcg ccgaggcccg gccccgccgg
     2941 ctcgtccgag aaatcgagcg tccagtaacg gcgcagcgtg cagccgtggc gatttgccag
     3001 caaataatgc cccggcggca cttcctccac accccggaac gccgtgcgcc ccggcagcgg
     3061 cgcccagtag gtgaacacct ggtcgagcgc ctccaggtcc agcgcgcgcg gcacgcgcgg
     3121 atcggccagc agggccttga tttcggaggc aaacagcagc gcgccaggca cctcggcgta
     3181 aaacagcggg cgcaccccca gccggtcgcg ggccagcagc agcgtgcccc ggcggcggtc
     3241 ccatagcgcg aaggcgaact ggccgatcag gtcgtgcacg caggccgggc cgtggtcctc
     3301 gtacaggtgg acgatgacct cgctgtccga gccggtgcgg aactggtggc cgcgggcgat
     3361 cagggccgcg cgcaactcca cgtagttgaa cacctcgccg ttgcagatga tccagatcga
     3421 gccgtcttcg ttggggatcg gttgcaggcc gccggccagg tcgatgatgc tcaggcggtc
     3481 gtggcccagg cccaccggcc cgtccagcac catgcccgcc gtctccggcc cgcgatggtg
     3541 gatcgcgccc agcagcgcat tgatctgcgc cggcgcgacc ggctgttgcg gatcgcgatt
     3601 aaagattccc gctatgccac acatcgccgc ttgctctccg tgtctaggac gcatgggtcc
     3661 ggccacagat ccgccatccc ccttttcccg atactcccat tgtaggccaa gagccttaac
     3721 gttcagtttt cggcaggacc taaatagtta cagtattgaa taaacttgcg ctgatcccca
     3781 gtggcttgca agaacagggc tatttctgta ataataaagg ggacagacca tccccttgcc
     3841 gcccacttat gcctggattg acaattatca agcggtagtg atacactgcg gacagcgatt
     3901 ggcgctggac ggaggacacc cccatgaaga tactgatggt cgatgacgac ccttcgctcg
     3961 tcaagattat cacctttttg ctcaaagacg aaggttacga cgtaattagc gcttccacgg
     4021 ggctggaagc gctgcagatc ctgcaacagc aatgggttga catgctgatc ctggatatcg
     4081 ggtt
//
LOCUS       DASUYC010000892         5673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_145792,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000892 DASUYC010000000
VERSION     DASUYC010000892.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5673
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145792"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="VF276_14585"
     CDS             complement(<1..927)
                     /locus_tag="VF276_14585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608129.1"
                     /translation="MTDTAVTGTGADALTAAVTRMARVGLCTSPSFSPDGSRLAFVSD
                     LNGIPQVWTVDATGGWPDLVTALDDQISAVRWSPDGAWLAFLLAPGGGMNQQVYLVRP
                     DGSDMRRLTDGGKETNWLDLWAHDGRTLALASNRRTSAAMDAYLVDVASGALRLVAEN
                     QGIGSIDDISRDGRWAVLNRMRQRSDNDLFLIDLANGTETLLTPHEGPGSFDHGTFSP
                     DGRTVYLSSNKDRELIAFARLPLDGAGQPGPIEVIAARDGAELQEFAVSEDGTTAALV
                     WNAGGRSDLSFLDLATGAATPGPALPAEIAMGV"
     gene            complement(965..3523)
                     /locus_tag="VF276_14590"
     CDS             complement(965..3523)
                     /locus_tag="VF276_14590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX6608130.1"
                     /translation="MADTIPQPPSAEKPSPAPPARRRVVLAAPGTRVAPEPAAPVPAP
                     EPAPPVSKEVPTLAVEAPREEQSSRGAFAGLWAVRGGWLGPVAWLLALAGTLALAAEA
                     TRTAGEIAIVVAGVLAVVAWGSVRAVPAFVAPHAAGRWVPGRRHLLCLGGIALAAALT
                     WAADARFLAGPAEAFGVPGWLWLAAMAVLPAAALAWPRPAPVAGGGSDPAPEPVSRRS
                     VLVDAAILAGITVLAVGTRVWDLAALPFAIHPDEILTGLLAYQHYIDGQGTPIFSTVW
                     TDINLPALWFWLVGQSLQLGGGTLAALRLPAALFGAATVIPFYGLVRMHWGRAAAVAG
                     SVILAVSASNVHYSRVTLNNIVTPFFWAVCCYFLLRGLRTRRPVDWVLAGFAGGLSEY
                     GYYGTHLLPFILLAFGAYLLVVHWRRAAEYAGRLGWLVLGYVTAFGPLLAYYTQHPTI
                     YFGRGAGVLMWDHIPRDWADLQLMWNTLWPLMAENLLGISTHPSQDSFYWAPLLLPAE
                     AALLVLGIALLIWHWRQPAAFLMLLVGTGVLFVGGTLVHGVPFLAHWTPAFPAFYAAL
                     AVPVSAWAASWPRSRQPLRLAGMGTLVLGLAALVWLNVDFYFNQYQVTRPEFELPAFE
                     GRWAASLGPDYQVYSVGRTWQPYNPELMRYLAPDQVGAALDDPASALPLPAPPGKGLA
                     FFFLADTQQYQAPVQALYPGGTTGEVRSHGGVHLFNTYVVPPDRVAAAYGVTLELAAP
                     DGSYHWTGRVRRVGQWLPETRGPLAARWTGALYLPHAGLYHISVNGAPAQLTIDGRPM
                     PPDGRQLGPGWHPFTVQTDPAPPAGLVLQIAEGSGPAAEVSPDHLWPVTPALGK"
     gene            3726..5516
                     /locus_tag="VF276_14595"
     CDS             3726..5516
                     /locus_tag="VF276_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608131.1"
                     /translation="MRTRLWRILWAGAALSLLLTAVPRPPATHAAPAADDATRADLFG
                     MVARDPYYEYNSDPQKFPDAPNRVALERQAAELQEMGVRWVRMEFFAEGDINWDRYDL
                     FINDIAPRHGLKVLGLLNAGIVSYEGGTVDPSRFNDPADQDDGSNHYIRVFRWRAQQI
                     AQRYGTAISAYEVVNEPNINYELWNSSNRHTAEIKPERYATLLTMTYRNIKTVAPEAQ
                     VLIGGMLLGPPPNDNSHDELDWLYQLYVAPAVQAYQASGDAGRPGWNVVPWDGVGLHP
                     YWVDAGKFQQLVRVMAQKLRDRGDFRSRIWVTEIGMEATPAPATAPPSDAEVAQANFL
                     TAVYRAVLDDPQLRQDVHTIFWFKYEDFPAPRPAVNFGLMRLAETASGYAPSGDVTVH
                     KLAYRAYQALARPGGLPESRLEGQALAQNRFYFPATGQAIAPEFQAYWESKGGLPQFG
                     YPLIQPTLIRGYRTQIFERAVFEYHPENAPPFDVLLRRLGAETLTHAYAEAQPFPDAP
                     DRHYFPETHHSLGGKFLEYWLRTGGLAVYGYPLSEEVQELDPRTNTTYTVQYFERNRF
                     EYHPENAGTPYEIQLGLLGGDLLQGGLWWR"
BASE COUNT          960 a         2028 c         1909 g          776 t
ORIGIN      
        1 gacgcccatg gcgatctcgg cgggcagggc tggaccgggg gtggcggccc cggtcgccag
       61 gtccaggaag gacaggtcgc tgcgcccacc cgcgttccac accagggccg cggttgtccc
      121 gtcctcgctg acggcgaatt cctgcaactc ggcgccgtcg cgggccgcga tcacctcgat
      181 ggggccgggc tgccccgcgc catccagggg cagccgggcg aaggcgatca actcacggtc
      241 cttgttcgag gacaggtaca cggtgcgccc atcaggcgaa aaggtgccat ggtcgaaact
      301 gcccggcccc tcgtgcgggg tcagcagggt ttccgtccca ttcgccagat cgatcaggaa
      361 caggtcgttg tcgctgcgct ggcgcatccg gttcagcacc gcccaccggc cgtcccggct
      421 gatgtcgtcg atgctgccga taccctggtt ctcggctacc aggcgcaagg cgccgctcgc
      481 cacgtcgacc agataggcgt ccatcgccgc ggaggtgcgc cggttcgagg ccagggccag
      541 cgtccgcccg tcgtgcgccc agaggtccag ccagttggtc tccttgccgc catccgtcag
      601 gcgccgcatg tcgctaccgt cggggcgcac caggtagacc tgctggttca tgccgccgcc
      661 cggcgccagc aggaaggcca gccacgcgcc gtccggcgac cagcgcaccg cgctgatctg
      721 gtcgtccagc gccgtaacca ggtcgggcca gccgcccgtg gcatccaccg tccagacctg
      781 ggggatcccg ttgaggtccg agacgaacgc cagccgcgat ccgtcgggcg aaaagctggg
      841 cgacgtgcac aggcccacgc gggccatgcg cgtgacggcg gcagtcagcg catcggcgcc
      901 ggtgcccgta accgcggtat cagtcatggc cgatctcctt tgttcgggtg gccctggcgc
      961 gggcctattt tcccagggcc ggcgtcaccg gccagaggtg gtccggcgac acctcggcgg
     1021 cggggccgct gccctccgct atctgcaaga ccagccccgc gggcggggcc ggatccgtct
     1081 gcaccgtaaa gggatgccag cccggcccca gttgccgccc atccggcggc atgggccggc
     1141 cgtcaatcgt gagttgcgcc ggcgcgccgt tcacgctgat gtggtagagg ccggcgtggg
     1201 gcaggtacag cgcgccggtc cagcgggcgg cgagcggccc gcgcgtctcg ggcagccact
     1261 gcccgacccg gcgcacccgc ccggtccagt ggtacgagcc atccggcgcg gccagttcga
     1321 gcgtgacccc gtatgccgcc gccacgcggt ccggcggcac gacgtaggtg ttgaacaggt
     1381 ggactccgcc gtggctgcgc acctcgccgg tggtgccgcc cggatagagc gcctggaccg
     1441 gcgcctggta ttgctgagtg tcggcgagga agaagaaggc cagccccttg cccggcggcg
     1501 cgggcagggg caacgcactc gccgggtcgt ccagcgcggc acccacctgg tcgggcgcga
     1561 gatagcgcat cagttcggga ttatacggct gccaggtgcg gcccaccgag tagacctggt
     1621 agtccggccc caggctcgca gcccagcggc cctcgaaggc cgggagttca aactcggggc
     1681 gcgtgacctg gtactggttg aaataaaaat cgacgttcaa ccagaccagc gccgccaggc
     1741 cgagcaccag cgtgcccatc ccggccaggc gcagaggctg acggctacga ggccaactgg
     1801 cggcccacgc gctcaccggc acagccagcg cggcgtaaaa cgccgggaag gcgggcgtcc
     1861 agtgcgcgag gaatggcacg ccatgcacca gcgtgccccc gacgaacaac acccccgtcc
     1921 ccacgagcag catcaggaaa gccgccggct ggcgccagtg ccagatcaac aacgcgatcc
     1981 ctaataccag cagcgccgcc tcggcgggca gcagcaaagg cgcccagtag aagctgtcct
     2041 ggctggggtg cgtgctgatg cccagcaggt tctcggccat caacggccag agcgtgttcc
     2101 acatcaactg caggtcggcc cagtcgcgcg ggatgtggtc ccacatgagt acgccggccc
     2161 cgcgcccgaa gtagatcgtc gggtgctgcg tatagtaggc cagcagcggc ccgaaagcgg
     2221 tcacgtagcc gagcaccagc cagccgagcc ggcccgcata ttccgcagcg cgccgccagt
     2281 ggacgacaag cagatacgca ccgaatgcca gtaggatgaa gggcagcagg tgcgtgccgt
     2341 aatagccgta ctcactgagg ccgcccgcga acccggcgag cacccaatcc accgggcggc
     2401 gggtgcgcag gccgcgcagc aggaagtagc agcagaccgc ccagaaaaac ggcgtgacga
     2461 tattattgag cgtaacccgg ctgtagtgga cgttcgacgc gctgacggcc aggatcacgc
     2521 tgcccgcgac ggcggcggcc cggccccaat gcatgcgcac caacccatag aaggggatca
     2581 ccgtcgccgc cccgaacagc gcggcgggca ggcgcaacgc cgccagggtg ccgccgccga
     2641 gttgcagcga ctggcccacc aaccagaacc agagcgcggg gaggttaatg tcggtccaga
     2701 cggtactgaa gatgggcgtg ccttgcccgt cgatatagtg ctggtaggcc agcagcccgg
     2761 tcaggatctc gtcggggtgg atggcgaagg gcagcgccgc cagatcccag acgcgcgtcc
     2821 cgaccgccag cacagtgata ccggccagga tggccgcgtc caccagcacc gagcgacgcg
     2881 agacaggttc cggtgcggga tcggacccgc cgcccgccac cggtgcaggt cgcggccagg
     2941 ccagcgcggc ggccggcagc acggccatgg ccgccaacca gagccatccc ggcacgccga
     3001 aagcctcggc ggggccggcc aggaagcggg catcggccgc ccaggtcagc gcggcggcca
     3061 gcgcgatgcc ccccaggcag agcaggtgcc ggcgaccggg cacccaccgg cccgcggcgt
     3121 gcggggcgac aaacgcggga acggcccgca cactgcccca ggcaactacg gccagaacgc
     3181 cggccacgac gatggcgatc tcgcccgccg tccgcgtcgc ctcggcggcg agggccagcg
     3241 tgcctgccag cgccagcagc caggccaccg ggcccagcca gccgccgcgc accgcccata
     3301 ggcccgcaaa cgccccgcgt gaggattgtt cctcgcgggg cgcctccacg gcgagcgtcg
     3361 gtacctcttt agagacgggc ggggcgggtt ccggcgcggg caccggcgcg gcgggttccg
     3421 gtgcaactcg ggtgcccggc gccgcgagta cgacgcgccg ccgggcaggc ggcgccggcg
     3481 agggtttctc cgcagatggg ggctgtggaa tcgtgtcggc catagacctc ctcgtaaggc
     3541 gacgatgggc cagactgggt cggcttgcgc cggtccgccg ggcaggcggg gtggttccat
     3601 tatacatcag aaagatcccg accaaatcgc gtttcatgca cgcgctaatg caacctaccg
     3661 gcaggccatt cgtcataatg gtgttgtacg ctggtgtgat tccccgtgtt ggaaaggacc
     3721 cgaacttgcg aacccgtctc tggcgcatcc tctgggccgg cgccgcgctg agcctgttgc
     3781 tgaccgccgt gccccgcccg cccgccaccc acgcggcgcc cgccgcggac gatgccacgc
     3841 gggccgatct cttcggcatg gtcgcccgcg atccctacta cgaatacaat agcgacccgc
     3901 agaagttccc tgacgcgcct aatcgcgtgg cgctcgaacg ccaggccgcc gaactccagg
     3961 agatgggcgt gcgctgggtg cgcatggagt tctttgccga gggcgatatc aactgggacc
     4021 gctacgatct cttcatcaac gacatcgccc cgcgccatgg cctcaaggtg ctgggcctgc
     4081 tcaacgccgg gatcgtgagc tacgagggcg gcacggtgga cccgtcgcgc ttcaacgatc
     4141 ccgccgacca ggacgacggc agcaaccact acatccgcgt gtttcgctgg cgcgcccagc
     4201 agatcgccca gcgctacggc acggccatca gcgcctacga ggtggtcaac gagccgaaca
     4261 tcaactacga gttgtggaac tcgtccaacc gccataccgc cgagatcaag cccgagcgct
     4321 acgcgacgtt gctcacgatg acctaccgca acatcaagac cgtcgcgccc gaggcccagg
     4381 tgctgatcgg cggcatgctg ctggggccgc cgcccaacga caacagccac gacgaactcg
     4441 actggctcta ccagctctat gtcgcgcccg ccgtgcaggc ctaccaggcc agcggcgacg
     4501 cggggcggcc cggctggaac gtggtgccct gggacggggt ggggctgcat ccctactggg
     4561 tcgatgccgg caagttccag caactggtgc gcgtcatggc gcagaagctg cgcgaccggg
     4621 gcgacttccg cagccggatc tgggtcacgg aaatcggcat ggaggctaca ccggccccgg
     4681 ccactgcccc gccctcggac gctgaggtcg cgcaggccaa cttcctgacc gccgtctacc
     4741 gcgccgtgct cgacgacccg caattgcgcc aggatgtgca taccatcttc tggtttaaat
     4801 acgaagactt ccccgcgccg cggcccgccg tgaacttcgg cctgatgcgc ctggcggaaa
     4861 ccgccagcgg ctatgcgccg tcgggcgacg tgacggtgca taagctggcc taccgcgcct
     4921 accaggccct ggcgcgcccc ggcggcctgc cggaaagccg cctcgaaggc caggccctgg
     4981 cccagaaccg cttctatttc ccggccacag gccaggccat cgcgccggag ttccaggcct
     5041 actgggagag caagggcggt ctgccccagt tcggctatcc gctgatccag ccgaccctga
     5101 tccgcggcta ccggacgcag atcttcgagc gcgccgtctt cgagtatcac ccggagaacg
     5161 ccccgccctt cgacgtgctg ctgcgccggc tcggcgccga gacgctgacc catgcctatg
     5221 cggaagctca gcccttcccc gatgcgcccg accggcacta cttcccggag acccaccact
     5281 cgctgggcgg caagttcctg gaatactggc tgcgcaccgg cggtctggcc gtctacggct
     5341 acccgctgtc cgaggaggtg caagagctcg atccgcgcac gaacacgacg tataccgtgc
     5401 aatacttcga gcgcaaccgc ttcgagtacc acccggaaaa cgccggcacg ccctacgaaa
     5461 tccagctcgg cctgctgggc ggcgacctgc tgcaaggcgg cctctggtgg cgctagtgag
     5521 cttccggctt cacagcacgg tgtaggggcc ggtttcaaac cggcctgtcg cctaatggct
     5581 cagcttccac agcgcagtgt aggggccggt ttgaaaccgg cctatcgccc aaagatgcgg
     5641 cttcccaacg cggtgtaggg gccggtttga aac
//
LOCUS       DASUYC010000893         2332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1539036, whole genome shotgun sequence.
ACCESSION   DASUYC010000893 DASUYC010000000
VERSION     DASUYC010000893.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539036"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..1226)
                     /locus_tag="VF276_14600"
     CDS             complement(30..1226)
                     /locus_tag="VF276_14600"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX6608132.1"
                     /translation="MVKRTLDDLDLKGKHALVRVDFNVPLDKKTNAITDDTRMRAALP
                     TIKALLDRGAAVILMSHLGRPKGVTPNLSLEPVAAHLRDLLPGVPVHFVADSVGDAAE
                     AAAKTLKPGEVLVIENLRFYKEEEANDPEHARRLAALGDVYVNDAFGSAHRAHASTEG
                     VAHLLPSALGLLMEKEVGIMGQALESPKRPFVAVLGGAKVSDKMGVITNLLTKVDSLV
                     IGGGMANTFLRAQGYNIGDSLVEADLVPEAEKLLTQAREKGVSLLLPQDVVIADAFSA
                     DAHIRTVPVSEVPDGWRIMDIGPDTAQRYAETIEKAGTVVWNGPMGVFEFAPFAAGTR
                     ALAEATARATDKGATTIIGGGDSVAAVEQAGVADRISHISTGGGASLEFLEGRVLPGV
                     AAIPER"
     gene            complement(1353..2312)
                     /gene="gap"
                     /locus_tag="VF276_14605"
     CDS             complement(1353..2312)
                     /gene="gap"
                     /locus_tag="VF276_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX6608133.1"
                     /translation="MREAYPDEIEVVAVNDLTDAETLAHLLKYDTNYGETDEDISVDG
                     NAIIVDDQRIMVTAEKDPANLPWKDLGVDIVIESTGRFTDAQKAAAHLQAGAKKVIIT
                     APAKNEDITIVMGVNEEKYDPARHNVISNASCTTNCLAPVAKVILDNFGIVRGMMTTV
                     HSYTNDQVILDFPHKDLRRARAAAQNIIPTTTGAAKAVALVIPELKGKFDGFALRVPT
                     PTVSIIDFVAETEQAVTVDSVNQALKDAALNDDAEYDVLDFTEEPLVSTDFRGDPHSA
                     IVDGLSTMVMGDKMVKVVAWYDNEWGYSCRVADLTAYVAEKGV"
BASE COUNT          350 a          745 c          795 g          442 t
ORIGIN      
        1 aataatcggc tgacgatggc ggctcatggc tagcgctccg ggatagcggc cacgccgggc
       61 aggacgcgtc cttcaaggaa ttccagcgac gcgcccccgc cggtggagat gtggctgatg
      121 cgatcggcca cccccgcctg ctcgaccgcg gcaaccgagt cgccgccgcc gatgatcgtg
      181 gtcgcgcctt tgtcggtggc gcgggccgtg gcctcggcca gcgcgcgggt gcccgccgcg
      241 aagggggcga actcgaacac gcccatgggg ccgttccaga cgaccgtgcc tgccttctcg
      301 atggtctcgg cgtagcgctg ggcggtgtcc gggccgatgt ccatgatgcg ccagccgtcg
      361 ggcacctcgc tcaccggcac ggtgcggata tgggcatcgg cggagaaggc atcggcgatg
      421 accacgtcct gcggcagcag caggctgaca cccttctcgc gggcctgggt cagcagcttt
      481 tcggcttcgg gcaccaggtc ggcctcgacc agcgagtcgc cgatgttgta gccctgagcg
      541 cgtaggaagg tgttcgccat gccgccgccg atgaccaggc tgtccacctt ggtcagcagg
      601 ttggtgatga cacccatctt gtcgctcacc ttggccccgc cgagcacggc gacgaacggg
      661 cgcttgggcg attccagcgc ctggcccatg atgccgactt ccttctccat cagcaggccg
      721 agcgcggagg gcagcaggtg ggccacgccc tcggttgagg catgggcgcg gtgggcgctg
      781 ccgaaggcgt cgttgacata cacatcgccc agggcggcca gccggcgggc gtgctcggga
      841 tcattggcct cttcttcctt gtagaagcgc aggttctcga tcaccagcac ctcgcccggc
      901 ttgagggtct tggccgccgc ttcggcggcg tcgcccacgc tgtcggcgac gaagtggacc
      961 ggcacgccgg gcagcaggtc gcgcaggtgc gcggccaccg gctccagcga caggttgggg
     1021 gtgacgccct tgggccgccc caggtgcgac atcaggataa cggctgcgcc ccggtcgagc
     1081 aacgccttga tcgtcggcag cgccgcgcgc atgcgggtgt cgtcggtgat ggcattggtc
     1141 ttcttatcca ggggcacatt aaaatcgacg cggaccaggg cgtgtttgcc cttcaggtcc
     1201 aggtcgtcca gagtacgctt aaccatgatc tgctcctcat aaacataatc agccgccaac
     1261 tctcgcccga aatgcctcac cccgctccgc gagcagtgaa aagctgttca agctgagcgt
     1321 tggcggctga ttaccgatag ctcgcgggtg ggctagacgc ctttctcggc cacgtaggcg
     1381 gtcaggtcgg cgacccggca ggaatagccc cactcgttgt cgtaccaggc caccaccttc
     1441 accatcttgt cgcccatcac catggtggac agcccgtcga cgatggccga atgcggatcg
     1501 ccgcggaagt ccgtcgagac cagcggttcc tcggtgaagt cgagcacatc atactcggca
     1561 tcgtcgttca gcgccgcgtc cttgagcgcc tggttgacgc tgtccaccgt caccgcctgc
     1621 tcggtctcgg ccacgaagtc gatgatcgag acggtgggcg tgggcacgcg cagcgcgaag
     1681 ccgtcaaact tgcccttcaa ctccgggatc accagggcca cggccttggc cgcgccggtg
     1741 gtcgtcggga tgatgttctg ggcggcggcg cgggcgcggc gcaggtcctt gtgcgggaag
     1801 tcgaggatca cctggtcgtt ggtgtaggag tggacggtgg tcatcatgcc gcgcacgatg
     1861 ccgaagttgt ccaggatcac cttggcgacc ggcgccaggc agttggtggt gcaggaggcg
     1921 ttggagatga cgttgtgccg ggccgggtcg tacttttcct cgttgacgcc catgacgatg
     1981 gtgatatcct cgttcttggc gggggcggtg atgatcacct tcttggcccc ggcctggagg
     2041 tgggccgccg ccttctgggc gtcggtgaac cgtccggtcg actcgatcac gatgtcgacg
     2101 ccgaggtcct tccagggcag gttggcgggg tctttttccg ccgtgaccat gatgcgctga
     2161 tcgtccacga tgatggcgtt gccgtcgacc gagatatcct cgtcggtctc accgtagttg
     2221 gtgtcgtact tcagcagatg ggccagggtt tcggcgtccg tcaggtcgtt cacggcgacg
     2281 acttcgatct cgtcgggata cgcttcgcgc agtgccttga atacttgccg gc
//
LOCUS       DASUYC010000894         2179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1763590, whole genome shotgun sequence.
ACCESSION   DASUYC010000894 DASUYC010000000
VERSION     DASUYC010000894.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1763590"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            536..1219
                     /locus_tag="VF276_14610"
     CDS             536..1219
                     /locus_tag="VF276_14610"
                     /inference="COORDINATES: protein motif:HMM:NF024919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEX6608134.1"
                     /translation="MGFPSRPPRRFVRRFLLVCVFSLMIPAGLAPTAGAAPVALPSKA
                     IIPRFPSVREIYNLSCEYAAASAITQFYGQRVTQGTFIREVPHAANPHLGFRGAIDAK
                     WGGITNYGIYAEPLVPVLERHGYEAEVFYDSPTRLKAEIAAGHPVVVWITGAARPGAR
                     TYKLDSTNTLYYLVPYEHAVVVYGYDSDRVYTMDVGDGKFWGYRWAKFMRSWDLFNDM
                     ALAIHPVQE"
     gene            1230..>2179
                     /locus_tag="VF276_14615"
     CDS             1230..>2179
                     /locus_tag="VF276_14615"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6608135.1"
                     /translation="MTTALPDGVTAPLLPRRARLAVTILFLAHGLVFASWLVRIPDVK
                     AQLRLSDGELGLALLGAPGGVFLGQFLVGWLLPRWGSRNIITLMALGWCTVFPLLGLA
                     PNLPLLMLGLVLYGLFGGGMDVAMNAQAAFVEGRYGRPIMAGFHGMWSVAGMAGAAVG
                     GWLASRGLPVELHFLAVVAVALPAVVLARRGLSVDLQAPAGAAQPFALLPQALLPLGV
                     IAFSVLLCEGAIGDWSTVYLRESLGSAPGLAATGYVVFSLLMTAGRLTGDWLTLRFGP
                     ARIVRAGGLLVLAGMAVILLTQAPIAAIVGFGLIGAGVACP"
BASE COUNT          307 a          706 c          716 g          450 t
ORIGIN      
        1 cacccaagat tcgatggaac catctcaacg tgcgaagcac gccggaaccg gttcgttgga
       61 aaagattggg ctattgtgtg aacttgactg gttggtgtca ctttgtgtga gcttgcttgc
      121 tcaccactct gctgttgtta aggtactgcc cgcttcgcgc ggtgcctcct attatatcag
      181 agattgcccg gctgtgtcaa gttgaccgct accgcgctga ggcaacaaaa aaccgccgtc
      241 tgaaaggacg ccgggagggg cgaaacacat ccatcgtcgc aagttgctgg tggtgtatcc
      301 gggcccattg cctcctctga tcttaggctg cgaaggtttt ctcaccgtag tgagcgaaac
      361 ctgggtgcgt cgtgaatttc acaacgcccc gttattataa tcaatcaccg cttcggagtc
      421 aagccttttg ccggccaatt tgggccggat ttgccgctgc ctggcggggg aactgtgcta
      481 taatggcggc tggtagtccc cgacctgcgt ttctcactgc tacaggagtt gttccatggg
      541 ttttccctcg cggccacccc ggcgattcgt tcgccggttt ctgctcgtct gcgtcttcag
      601 cctgatgatc ccggcggggc tggcaccgac cgccggcgcc gccccggtcg ccctgccgag
      661 caaagccatc atcccccgtt tcccctcggt gcgcgaaatc tataacctga gttgcgagta
      721 cgccgccgcc tcggccatca cccagtttta cgggcagcgc gtcacccagg gcacctttat
      781 ccgcgaagta ccgcacgcgg cgaacccgca cctgggcttc cgcggcgcaa tcgacgccaa
      841 gtggggcggc atcaccaact acgggatcta cgccgagccg ctggtgccgg tgttggaacg
      901 ccacgggtat gaggccgagg tcttttacga ctcgcccacc cggctcaaag ccgagatcgc
      961 ggcggggcac ccggtggtcg tctggataac gggggcggcg cggcccggcg cccgcacgta
     1021 caagctcgac agcaccaaca cgctctatta cctggtgccc tatgagcacg ccgtggtcgt
     1081 ctacggctac gactccgacc gcgtctacac catggacgtg ggcgacggca agttctgggg
     1141 ctatcggtgg gccaagttca tgcgaagctg ggatctgttc aacgacatgg ccctggcgat
     1201 ccacccggtc caggaatagc cccgccgcca tgacaacggc cctgccggac ggcgttaccg
     1261 cgccgctgct gccgcggcgg gcgcggttgg ccgtcacgat cctctttctg gcccatggcc
     1321 tcgtcttcgc cagttggctg gtgcgcatcc ccgatgtcaa agcccagttg cggctgtcgg
     1381 acggcgaact ggggctggct ctgttagggg cgccgggcgg cgtcttcctg ggccagttcc
     1441 tggtcggctg gttactgccg cgatggggca gccggaacat catcaccctg atggcgctcg
     1501 gctggtgcac agtgttcccg ttgctcggcc tggcgcccaa cctgccgctg ctcatgctgg
     1561 ggctcgtcct ctatgggctc ttcggcgggg gcatggatgt cgccatgaac gcccaggcgg
     1621 ccttcgtcga gggccggtat gggcggccca tcatggccgg gttccacggc atgtggagtg
     1681 tggcgggcat ggccggcgcc gcggtgggtg gctggctggc gagccgcggg ctgccggtgg
     1741 aactccattt cctggccgtg gtggcggtag cgttgccggc tgtcgtcctg gcccgacggg
     1801 gcttgagtgt cgatctgcaa gcccccgccg gcgcggcgca accgttcgcc ctgctgccgc
     1861 aggcgttgtt gcccctcggt gtgattgcct tcagcgtctt gctctgtgag ggtgccatcg
     1921 gcgactggag cacggtctac ttgcgcgaga gcctgggcag cgcgccgggc ctggccgcta
     1981 cgggctacgt cgtcttctct ctgctcatga ccgccgggcg cctgacgggc gattggctca
     2041 cgctacggtt cgggccggcc cgcatcgtgc gcgccggggg cttgctcgtg ctggccggca
     2101 tggccgtcat cctgctgacc caggcgccca tcgccgctat cgtcgggttt gggttgatcg
     2161 gcgcgggcgt ggcgtgccc
//
LOCUS       DASUYC010000895         2136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_850437,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000895 DASUYC010000000
VERSION     DASUYC010000895.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850437"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VF276_14620"
     CDS             <1..229
                     /locus_tag="VF276_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEX6608136.1"
                     /translation="YSAGTFATCAEATAACRRIVDEFLLDQYKPGMAAEELCSLYTGF
                     GEDPFIISDDPACRFSAWTYARDRCQEICSR"
     gene            439..1329
                     /locus_tag="VF276_14625"
     CDS             439..1329
                     /locus_tag="VF276_14625"
                     /inference="COORDINATES: protein motif:HMM:NF018506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HEX6608137.1"
                     /translation="MAGLFYALITVLGWGTWLVPSQKVVFPSQQIKTLYVVATNLAIA
                     TVVALGQGSLGGLSTGTFWLTLIGGVIWAGGGWGAFTATNELGIAKAFGIWAPLNIIV
                     SLIWGAVLFQEFVNLSAGTILWLGVAILIILVGVLLIIFSKGTGAGGQDHRALRWGLT
                     GAVGAGVLWGSYFIPVKYSGVSAWAGAFPLAIGMAVGGLILALLSRQSWKLPATGDYV
                     RACLTGALWSAGNYGALLLIGEIGAGKGFTISQTSLVVSALISIYWLHEPPPKTRAAR
                     WTGIGCVLATIGGIVLGNLK"
     gene            complement(1510..2004)
                     /locus_tag="VF276_14630"
     CDS             complement(1510..2004)
                     /locus_tag="VF276_14630"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX6608138.1"
                     /translation="MDSQPKLTELQLDAWSAFLTAHARVIGRIDAELAAAGRLPLGSY
                     DVLIELAGAPEHRLRMSRLARQVVLSNSGLTRLVDRLEKQGLLRREVAPTDRRGAYAV
                     LTAEGLAALREAWPVYARGIAAHFGRYLSPDEATTLATALDRIAEAAATDAPRSFNET
                     LRED"
BASE COUNT          364 a          660 c          702 g          410 t
ORIGIN      
        1 gtacagtgcg gggacgtttg cgacctgcgc ggaggccacg gcggcctgcc ggcgcatcgt
       61 ggacgagttt ctgctcgacc agtacaagcc cggcatggcc gccgaagagc tgtgcagcct
      121 ctacaccggc ttcggcgaag atccattcat catcagcgac gatcccgcct gccgtttctc
      181 ggcctggacc tatgccaggg atcgctgcca agagatttgc tcgcgctaaa gccgaaacac
      241 gaccggaaca cacctcgcta catcatccac gttacccatc cagcagactg cacgcgttgc
      301 ggtgcggcga cgggcgggcc gcgtacgtgc gcgaaagcgg gccggtaggg gcgccgccgt
      361 tccccggcgt tccagaaccc ggagcttgac agccgttcgt tgccgccacg atctgccggg
      421 caacaggagg agcgaatcat ggccgggcta ttctacgcgt tgattaccgt ccttggttgg
      481 ggaacatggc tggtgccgtc gcaaaaggtt gtatttccca gccagcaaat caagaccttg
      541 tatgtggtgg cgaccaatct cgcgattgca acggtggtgg cgctcgggca aggcagcctg
      601 ggtgggctga gtaccggcac gttctggctg accctgatcg gcggcgtgat ctgggccggc
      661 gggggctggg gcgcgtttac cgctacgaac gaactgggga tcgccaaagc gttcggcatc
      721 tgggctccgc tcaacatcat cgtcagcctg atttggggcg cggtcctgtt ccaggaattt
      781 gtgaacctga gcgcggggac tatcctgtgg ctgggcgtcg cgattctgat cattctggtg
      841 ggcgtcctgc tgattatttt ctcgaagggc accggcgcgg gcggccagga tcaccgggcc
      901 ctgcggtggg gcctgaccgg ggcggtgggc gcgggcgttc tgtggggtag ctatttcatt
      961 ccagtgaaat attcgggtgt gtcggcgtgg gcgggtgcct ttccgttagc gatcggcatg
     1021 gcggttggcg gcctgattct ggcactgctg tcgcggcaat cgtggaaatt gccggcgacc
     1081 ggcgattacg tgcgcgcctg tttgaccggt gcgctctgga gcgcgggcaa ttacggtgca
     1141 ctgttgctga ttggtgaaat aggggcgggc aaaggtttca ccatctctca gacttcgctc
     1201 gtcgtcagcg cgctcatcag tatttactgg ctgcatgaac cgccgcccaa aacccgcgcg
     1261 gcccggtgga ccgggatcgg gtgcgtcctg gcaaccatcg gggggattgt gctgggcaat
     1321 ctgaagtagc agcagggacg ggaaagccga acacccccgg tgacatgtca ccgggggtgc
     1381 ccatcgtatg cgatcagcga cggcggggag cacttgccga ccgcggccac gtccgacaag
     1441 tatcttctga ctgcccgtcc gccccacgcc gctgcactct tattatcgca ttcctgcctt
     1501 aaactcagct taatcctcgc gcagagtctc attaaaagat cggggcgcgt ccgtcgccgc
     1561 cgcctcggcg atccgatcca gggccgtggc aagggtggtg gcctcgtcgg gactgagata
     1621 gcgcccgaag tgggcggcga tgccgcgggc gtagaccggc catgcctcgc gcagtgcggc
     1681 cagcccctcg gcggtgagca cggcgtaggc cccgcgccga tcggtgggcg cgacttcgcg
     1741 gcgcaacagc ccctgcttct ccaggcggtc caccaggcgc gtcagcccgc tgttgctgag
     1801 caccacctgg cgcgccagcc ggctcatgcg caaccggtgc tccggcgctc cggccagttc
     1861 gatcaacaca tcatacgagc cgagcggcag gcgccccgcc gcggccagtt ccgcgtcgat
     1921 acgcccgatc acccgcgcgt gcgccgtgag aaaggcgctc caggcatcta actgcagttc
     1981 cgtgagtttt ggctgagaat ccatgccctg attatactcc aaagtacttg catccgcaaa
     2041 tattttgtgc tatactgcga aagtagttgc acagacaaat attgtcaggg caacaaaacc
     2101 gctgactatc gcgtgggccg caggcccgcg tcccga
//
LOCUS       DASUYC010000896         2749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_844895,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000896 DASUYC010000000
VERSION     DASUYC010000896.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844895"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VF276_14635"
     CDS             complement(<1..594)
                     /locus_tag="VF276_14635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2236 domain-containing protein"
                     /protein_id="HEX6608139.1"
                     /translation="MRYTEEFLDALRQEGDPLADRVIAEVHATGAVGAVNAVLRQLME
                     NDQPIPENLPPLVHEYLRITDNPPAWMDPARMARANGFFESHGMHISLVMSTAGMIEC
                     YAARKAVKVLDFTHQLDHPQRRMAETAQFCLHMMSPQAFAPDGKFIPATQKVRLMHAA
                     FRHLILQSGRWPLDELGVPICQEDLLGALLLFSTEVLT"
     gene            complement(924..1517)
                     /locus_tag="VF276_14640"
     CDS             complement(924..1517)
                     /locus_tag="VF276_14640"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX6608140.1"
                     /translation="MSATSPVPQSVIDEFVGVAHGDFQRLQELLAEYPAVLNGDATWH
                     ETAIEAAAQMGRVDMVEYLLAAGAPLDICTAAMLGRRAEVAAALDADSAAAHATGAHG
                     IPVLYYPVIANHPAIAEDLLARGADVNAGAGGNTPLHGAAMFGQPEMAAWLLAHGADP
                     AARDYNGKTPLEVALDSKQAAVADLLRAHSPAPGNEA"
     gene            complement(1542..>2749)
                     /locus_tag="VF276_14645"
     CDS             complement(1542..>2749)
                     /locus_tag="VF276_14645"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX6608141.1"
                     /translation="GSRSVPMFSKRTTWNLTGNAWAALAARKRAAGTPLLDLTESNPT
                     RCGFDYGGAGLDALADPAAVRYRPAARGAPAARAAVAAYYATHGLAVDPESVILTAST
                     SEGYSYLFKLLADPGDEVLAPLPSYPLFEYLAQLDGVRLAPYPLRYDGAWFIDFPALE
                     AAITPRTCAVIVVHPHNPTGAFLKQDELARLTAICRAHDLALICDEVFLDYAYGPDPV
                     RAPSLVAGGEVLTCTLSGLSKLAGLPQVKLSWIVLTGPPALQTAALARAEVIADTYLS
                     VSTPVQLALPALLGNDAALRAQIQARITANRRALDAALAGDLPLRALPAEGGWYATVQ
                     VPAILTEEAWALALLTEDDLLVHPGYFFDFPFGCLLVLSLLPPPDVFAAGVARLVQRV
                     AAVVAADDR"
BASE COUNT          425 a          898 c         1003 g          423 t
ORIGIN      
        1 cgtgagcacc tcggtactga acaacagcaa cgcgcccagc aaatcttcct ggcagatcgg
       61 cacgcccaat tcgtccagcg gccagcgccc gctttgcagg atgaggtggc gaaaagccgc
      121 gtgcatcagg cgcaccttct gcgtggcggg gatgaacttg ccgtcggggg cgaacgcctg
      181 cgggctcatc atgtgcaggc agaactgcgc cgtctcggcc atgcggcgct gggggtggtc
      241 gagctggtgg gtgaagtcga gcaccttgac cgccttgcgc gcggcgtagc attcgatcat
      301 gcccgccgtg gacatgacca ggctgatatg catgccgtgg ctctcgaaga agccgttggc
      361 ccgcgccatg cgcgcggggt ccatccaggc cggcggattg tcggtaatgc gtagatactc
      421 gtggaccagg ggcggcaggt tttccggaat cggctggtcg ttctccatga gttgccgcag
      481 caccgcgttg accgcgccca ccgcccccgt ggcgtggacc tcggcgatca cgcggtcggc
      541 caacgggtcg ccctcctggc gcagcgcatc cagaaactct tccgtatatc tcacagccgt
      601 tccccctgat cagatcggtc gtcgttgcgc gccgcccccg gtgccgggac ccgccgctgc
      661 gcgccggtcc cggcctacgc gcctgcctaa tggatgggat atcgcagggg caaggttacg
      721 cgggccgggg atacagggta attatagcaa acggggccgc cggatgctat ccgccacccg
      781 gatatgggtt tatcctcacc gcgaaggaac gaagggagcg aagaacaaac cttatataaa
      841 tactcttcgc gcccttcgcg tcttcgcggt ggactttaga atctctgcgt cctccgcgcc
      901 tcggcaatga ggataatccg ctgtcacgct tcgttgccgg gcgcggggga gtgggcgcgg
      961 aggagatcgg cgacggcggc ctgcttgctg tccagggcga cttccagcgg ggttttgccg
     1021 ttgtagtcgc gcgccgccgg atcggcgccg tgggccagca gccaggcggc catctcgggc
     1081 tgcccgaaca tggccgcgcc atgcagcggc gtattgccgc ccgccccggc gttcacatcg
     1141 gcgccgcgcg ccagcagatc ttcggcgatg gccgggtggt tggcgatgac cgggtagtag
     1201 agcaccggga tgccgtgcgc gccggtggcg tgggcggcgg cggagtcggc gtccagggcg
     1261 gcggcgacct ccgcgcggcg cccaagcatg gcggcggtgc agatgtcgag cggcgcgccc
     1321 gccgccagca ggtattcgac catgtcgacc cggcccatct gggcggcggc ctcgatggcc
     1381 gtttcatgcc aggtagcgtc gccgttgagc acggcggggt actcggccag cagttcttgc
     1441 aatcgctgga agtcgccgtg cgccacgccg acgaattcgt cgatcacgga ttgcggtacc
     1501 ggggaagttg cgctcatggg agtctccttt gcactagcga gtcaccgatc gtccgctgcg
     1561 acaaccgctg ccacccgctg cacgaggcgc gccacgcccg cggcgaacac gtccggcggc
     1621 gggagcaggc tgagcaccag caggcaacca aacgggaagt cgaagaaata gccggggtgc
     1681 accagcagat cgtcttcagt cagcagggcc agcgcccagg cttcttcggt caggatggcg
     1741 ggcacctgca ccgtggcgta ccagccgcct tcggcgggca aggcgcgtag cggcaggtcg
     1801 ccggccagcg cggcgtcgag cgcccggcgg ttcgcggtga tgcgcgcctg gatctgggcg
     1861 cgcagcgcgg cgtcgttgcc cagcagcgcg ggcaaggcca gttgcaccgg cgtgctcacc
     1921 gacaggtacg tgtcggcgat gacctcggcg cgggccaggg ccgccgtttg cagggccggg
     1981 ggaccggtca gcacgatcca gctcagcttg acctgcggca gcccggccag cttcgatagc
     2041 ccgctcagcg tgcaggtcag cacctcgccg ccggccacca gcgacggggc gcgcaccggg
     2101 tcggggccgt aggcgtagtc gaggaagacc tcgtcgcaga tcagcgccag atcgtgcgcc
     2161 cggcagatcg ccgtcaggcg ggcgagttcg tcctgcttga ggaaggcgcc ggtcgggttg
     2221 tgggggtgga cgacgatgac ggcgcacgtg cgcggagtga ttgccgcctc cagggccggg
     2281 aagtcgataa accaggcgcc gtcgtagcgc agcgggtagg gcgccaggcg cacgccgtca
     2341 agctgggcca ggtactcgaa cagcgggtag ctgggcagcg gggccagaac ttcgtcgccg
     2401 gggtcggcca gcagcttgaa caggtagctg tagccctcgc tggtgctcgc cgtcaggatc
     2461 accgattcgg gatcgacggc caggccgtgc gtggcgtagt aggcggcgac ggcggcgcgc
     2521 gcggcgggtg cgccccgcgc cgcgggccgg tagcgcacgg ctgccggatc ggccagggca
     2581 tccagaccag cgcctccgta gtcgaagcca caacgcgtgg gattcgactc ggtcagatcc
     2641 agcagcggcg tgcccgcggc gcgcttgcgc gcggccaggg ccgcccaggc gttgccggtg
     2701 aggttccagg tggtgcgctt ggagaacata ggcacagacc ggctcccgc
//
LOCUS       DASUYC010000897         4162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1072168, whole genome shotgun sequence.
ACCESSION   DASUYC010000897 DASUYC010000000
VERSION     DASUYC010000897.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072168"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1178
                     /locus_tag="VF276_14650"
     CDS             <1..1178
                     /locus_tag="VF276_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020477729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX6608142.1"
                     /translation="TWSALLALAPGATPEDLAARQREQEFDDPINIQYTSGTTGFPKG
                     ATLSHHNILNNGFFVGEMMRFTDQDRLCIPVPLYHCFGMVMGNLTCVSHGAAMVYPAE
                     SFDPLATLQAIEAERCTAVHGVPTMFIAELNHEEFDRFDLATLRTGIMAGSPCPIEVM
                     RQVNSRMHMGEVEIAYGMTETSPVSFQSRADDPIEKRVSTVGRIMPHLECKIVDPGTG
                     AVVPRGTPGELCTRGYSVMLGYWNNPEATATAIDPARWMHTGDLATMDAEDYVNIVGR
                     IKDMIIRGGENVYPREIEEFLYTHPAIADVQVIGVPSKAYGEEIMAWVKLKPGAEATA
                     EDVQSFCRGRISHYKIPRYVKFVDNFPMTVTGKIQKFLMRRQSIDELGLENEIQATA"
     gene            1380..2123
                     /gene="map"
                     /locus_tag="VF276_14655"
     CDS             1380..2123
                     /gene="map"
                     /locus_tag="VF276_14655"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX6608143.1"
                     /translation="MSIETEKDLTALRRIGEIVGLTLQHMQAQVQPGMTTRDLDAIGG
                     AFLKKYGARPAPLLVYKFPGIACISLNEEAAHGIPSDRTIREGDLVKLDLSAELNGYF
                     TDAAVTVPVPPLPTRKQQLCDCARATLDAALNAARAGAPINRIGAAAETTARRYGFQV
                     VRDLCGHGVGRGLHEAPSVPNYFVPRARQRLTEGLVLAVEPHVAAGRDAVVTDANGWT
                     IKTRDCSVVANFEHTIVVTRDRPLLVTAV"
     gene            2143..3222
                     /locus_tag="VF276_14660"
     CDS             2143..3222
                     /locus_tag="VF276_14660"
                     /inference="COORDINATES: protein motif:HMM:NF027495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HEX6608144.1"
                     /translation="MPLEIRLPVPHLLGGHCASSAFRDLVAYYSPPGSTTLPSEALLF
                     ALSGGLDFLYWRRPGADPPIYLLGRTGPLEAQLAARLGIGFEEVRPPSAEAAWAWLRA
                     TLAGGHPALVLADVGELPYLNARTHFTGHRLLVIGIDDPAGTLFVLDNDREGVQEITQ
                     AAFDASWRSTAFPAPVEYRYYEVRQPAALADPAAHIREAVAETVRLLDGHGIPFVDAA
                     QFAGVGGQGLAGLELLVADLARWPDHFAPAEVTQALRAVYLYVEKAGTGFGGFFRHIY
                     GTGLREAAALVADPALATGLEQAGAHYQALGQAWSAFAAACRAAAPAAGSDPAGTLAS
                     LLPQAAALLAAERAGRDLLAGVVAG"
     gene            complement(3219..>4162)
                     /locus_tag="VF276_14665"
     CDS             complement(3219..>4162)
                     /locus_tag="VF276_14665"
                     /inference="COORDINATES: protein motif:HMM:NF024778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX6608145.1"
                     /translation="ISHTIGVFVLGLIVLYASQYILPETLYPWLGFFSGLLIAGMGIA
                     MFWQRRNAWARSRRAAVPTPAPANGTPAYDPHEYAHAHGLPHEHDAHGLPHEHDAHAP
                     ALIPQPAPVPQLALSHAGHEHGHDHAIAHSHADHDHDHRHSHGDTLAYSHAAHAYDHD
                     HDHAHTLDHAHDHDLTHDHDHEHDALKPHKHGPFGREHSHVPADGQQVTIGSMLALGI
                     TGGIIPCPSALVVLLVAIASHRVGLGLLLIVAFSLGLACVLTGIGLLMVYGRGLLSRV
                     RMSGAVWGRLPMASALAVSCLGMLIAFEALNAAGLLR"
BASE COUNT          666 a         1449 c         1400 g          647 t
ORIGIN      
        1 ggacctggtc cgcgctgctg gccctggctc ccggcgccac gccggaggac ctggccgccc
       61 gccagcgcga gcaggagttt gacgacccga tcaatatcca gtacacctcc ggcactaccg
      121 gcttccccaa gggcgccacg ctgagccacc ataatatcct caacaacggc ttcttcgtcg
      181 gcgagatgat gcggtttacc gaccaggacc ggctctgcat ccccgtgccg ctttatcact
      241 gtttcggcat ggtcatgggc aacctgacct gcgtcagcca cggcgcagcc atggtctatc
      301 ccgccgagag cttcgacccg ctggcgacct tgcaggccat cgaggccgag cgctgcacgg
      361 cggtccacgg cgtgcccacg atgttcatcg ccgagttgaa ccacgaggag ttcgaccggt
      421 tcgacctcgc cacgctgcgc accggcatca tggccgggtc gccctgcccc atcgaggtca
      481 tgcgccaggt caacagccgg atgcacatgg gcgaagtcga gatcgcctac ggcatgaccg
      541 agacctcgcc ggtgtcgttc cagagccggg ccgacgaccc catcgagaag cgcgtcagca
      601 ccgtgggccg gatcatgccc cacctggagt gcaagattgt cgatccgggc accggcgccg
      661 tggtgccgcg cggcacgccc ggcgaactct gcacccgcgg ctattcggtc atgctgggct
      721 actggaacaa tccggaggcc accgccacgg ccatcgaccc ggcccgctgg atgcacaccg
      781 gcgacctggc gacgatggac gccgaggact atgtgaacat cgtgggccgc atcaaggata
      841 tgatcatccg cggcggcgag aatgtctacc cgcgcgagat cgaggagttc ctctacactc
      901 acccggccat cgccgacgtg caggtgatcg gtgtgcccag caaggcctac ggcgaagaga
      961 tcatggcctg ggtcaagctc aagcccggcg cggaggcgac ggcggaggac gtgcagagct
     1021 tctgccgggg ccgcatcagc cactacaaaa ttccgcgtta tgttaagttt gtggataact
     1081 tcccgatgac tgtcacgggc aagatccaaa agttcctcat gcgccggcag tccatcgacg
     1141 aactgggcct cgaaaacgag atccaggcaa cggcgtagcc ctcgcggcgg cagcgatacc
     1201 aggacaccgc gaaggcgcga agaacgcgaa ggcaatttat gttagctctg ttcttcgtgc
     1261 ccttcgcgcc ttcgcggtgt agccacggac ggatctcagg tagccgcctt gcgcgtattt
     1321 gcggagaggg gttatactac cagccgaggt gttttgtgcc gtagcccgga ggaagcgtga
     1381 tgtcgattga aaccgagaaa gatctgaccg ccctgcgccg gatcggggag atcgtgggcc
     1441 tgacgttgca gcatatgcag gcccaagtgc agccgggcat gaccacccgc gacctcgatg
     1501 ccatcggcgg cgcgttcctg aagaagtatg gcgcgcgccc ggccccgctg ctggtctaca
     1561 agttccccgg catcgcctgc atcagcctga acgaggaggc ggcgcacggc attcccagcg
     1621 accgcaccat ccgcgagggc gacctggtga agctcgacct gtccgccgaa ctgaacggtt
     1681 acttcaccga cgccgccgtg acggtgcccg tgccgcccct gcccacccgc aagcagcagt
     1741 tgtgcgactg cgcccgcgcc acgctggatg ccgcgctgaa cgccgcccgc gcgggcgcgc
     1801 cgatcaaccg catcggcgct gctgccgaga ccaccgcccg gcgctacggc ttccaggtgg
     1861 tgcgcgatct ctgcggccac ggcgtggggc gcgggctgca cgaggcgccc tcggtgccca
     1921 actacttcgt cccgcgcgcc aggcagcgcc tgaccgaagg gctggtcctg gccgtcgagc
     1981 cgcatgtcgc cgcgggccgc gacgccgtgg tgaccgacgc caacggctgg acgatcaaga
     2041 cacgcgactg cagcgtggtc gccaacttcg agcacaccat cgtcgtcacg cgcgaccggc
     2101 cgctgctcgt gaccgccgtt taggccccgc ccggtgcctg tgatgcccct ggagatccgc
     2161 ttgcccgtcc cgcatctgct gggcgggcat tgcgcatcgt cggccttccg cgacctcgtc
     2221 gcctattaca gcccgcccgg cagcactacc ctgcccagcg aagcgttgct gttcgcactc
     2281 tccggcgggc tggactttct ctactggcgg cgaccgggcg ccgacccgcc catctacctg
     2341 ctggggcgca ccggtccgct ggaggcgcaa cttgccgcgc ggctgggcat cggcttcgag
     2401 gaagtgcgcc cgccttcagc ggaggcggcc tgggcctggc tgcgcgccac gctggccggc
     2461 gggcatcccg cgctcgtcct ggccgatgtg ggcgaactgc cctatctgaa cgcgcgcacc
     2521 cacttcaccg gccaccgcct gctggttatc ggcatcgacg acccggcggg cacgttgttc
     2581 gtgctcgaca acgaccgcga aggcgtgcag gagatcaccc aggccgcctt tgacgcgtcc
     2641 tggcgctcaa ccgccttccc cgcgcctgtc gagtaccgct actatgaggt gcgccagccc
     2701 gcggccctgg ccgaccccgc cgcgcatatt cgcgaggcgg tcgccgagac ggtgcgcctg
     2761 ctcgacggcc acggcatccc gtttgtggat gccgcgcagt tcgcgggggt gggcggccag
     2821 gggctggcgg gcctggagtt gctcgtggcc gacctggccc gctggcccga ccacttcgcg
     2881 cccgccgaag tgacccaggc gttgcgcgcc gtctacctct acgtggagaa agcgggcacc
     2941 ggcttcggcg gcttcttccg ccacatctac ggcaccgggc tgcgcgaagc cgccgcgctg
     3001 gtcgccgacc ccgccctcgc caccgggctg gagcaggcgg gcgcgcatta ccaggcgctg
     3061 ggccaggcct ggagcgcgtt tgcggcggcc tgccgggcgg cagcccccgc cgccggaagc
     3121 gatcccgcag gcaccctggc gtccctgttg ccgcaggccg ccgcactcct ggcggcggag
     3181 cgggccgggc gcgatctcct ggccggggtg gtcgccggct aacgcaacag gcccgccgcg
     3241 ttcaacgcct cgaaggcgat cagcatcccc aggcaggaga cggccagcgc cgaggccatg
     3301 ggcaggcggc cccagaccgc gccgctcatc cgcacccggc tcagcaagcc acgcccgtag
     3361 accatcagca ggccgatgcc ggtcagcacg caggccagcc ccaggctgaa ggcgacgatc
     3421 agcagcaggc ccagcccgac ccggtgcgag gcaatcgcca ccagcaacac aaccagcgcc
     3481 gacgggcagg ggatgatccc gccggtgatg cccagcgcca gcatgctgcc gatggtgact
     3541 tgctgcccgt cggcgggcac gtggctatgc tcacggccga agggtccgtg cttgtgcggc
     3601 ttgagcgcat cgtgctcatg atcgtggtcg tgcgttaggt catggtcatg agcgtgatca
     3661 agggtgtgcg catggtcatg gtcgtgatca taagcatggg cggcatggct ataggctagg
     3721 gtgtcgccat ggctgtgcct gtgatcgtgg tcatggtccg cgtggctgtg cgcgatagcg
     3781 tggtcatggc catgctcatg gcccgcgtgg ctaagcgcca gttgcgggac cggggcgggt
     3841 tgcgggatca gcgcgggggc atgcgcgtcg tgctcatgcg gcaaaccgtg cgcgtcgtgc
     3901 tcatgcggca aaccgtgcgc gtgggcgtac tcatgcgggt catacgcggg agtgccgttt
     3961 gcgggcgcgg gagtcgggac cgccgcgcgg cggctgcgcg cccaggcgtt gcggcgctgc
     4021 cagaacatgg cgatgcccat cccggcaatc agcaggccgg agaagaagcc gagccagggg
     4081 tagagcgtct ccggcaggat atattgcgag gcatagagca cgatcaagcc cagcacgaac
     4141 acgccgatgg tgtggctgat cg
//
LOCUS       DASUYC010000898         9499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1764661, whole genome shotgun sequence.
ACCESSION   DASUYC010000898 DASUYC010000000
VERSION     DASUYC010000898.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 9499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9499
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1764661"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..361
                     /locus_tag="VF276_14670"
     CDS             95..361
                     /locus_tag="VF276_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608146.1"
                     /translation="MEHGSAGPVQGAPVYEIRVRGHLSPQLAAWFDDLTVTNQPQGEA
                     VLAGVLSDQAALYGVLFKLNNLGLTLLAVTPLAVPPAGVPDGAP"
     gene            604..3276
                     /locus_tag="VF276_14675"
     CDS             604..3276
                     /locus_tag="VF276_14675"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX6608147.1"
                     /translation="MTPGLVPRPRLRELLQQGLQRRLLLLSAPAGFGKTTLLAAWIHE
                     SVAPTMPVAWVTLDAGDNDPLRLGRYLCAALNQAQAGLAPQTTRLLQAPHPPALEAIL
                     TELVHELAARSQPLVLVLDDYQALTAPAIPPLLTFLLDQLPDTVHLVLASRIDPLLPL
                     ARLRVQRQLVEIRAGDLRFTADEATAFLNKFMGLALVPTAITLLAARTEGWIAGLQLA
                     ALSLQGRTPAGIAAFLAAFAGSHHFILDYLGDEVLHRQPPATQQFLLQTAILDRLSAS
                     LCDAVTESTGSQAMLEQLEQRNLFLIPLDEERHWYRYHQLFAEFLRSRLAAAGTLDVP
                     VAALHQRAARWYERHAFLDQAVEHALAAEDFAGAARLVEQYLVSGFRGGELETLLRWF
                     TALPAATTRPRARLSLYYAQVLLLSGRWAAVPHWLQAAEQSLAAQGASWPEGERRTVQ
                     GEVSAIRALFASLHAEVAQVDRLADDARRLLPPDHPLWAPLLLSQGSAYWLAGDLAAA
                     VGVLTQAGEAAQRTGNMYVFYIITVYLAQVRLAQGHLREAMRLQESGRQLVTARGREG
                     AEGNGLAVGLGALWCEGQALAEAQALIEQGLERAQQERNGIVVIGGKATLAWVRHAQG
                     QAQQAEALMQEAVTLGRQQGVDWTWITGPLRTAQARLWLAQGNLTAAAQWAAEPAGEA
                     PVPAYFAEAAAILQARLYRAQGRYGDALALLDRLQGPVTAAGHRQHLLEILVLRAGLA
                     QDQGQQGAARTTLRAAVDLAAPEGFVRTFVEGGPALAELLQSLRPVRAEQATYLARVL
                     RAFAPEAAPGPAAPPPPGLRSDDALSERELDVLRLMAGGASNQEIAAELVIAVNTVKR
                     HARNIFEKLDAANRTQAVARARDLGLLGPRDSPR"
     gene            complement(3638..4120)
                     /locus_tag="VF276_14680"
     CDS             complement(3638..4120)
                     /locus_tag="VF276_14680"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HEX6608148.1"
                     /translation="MAVSSAAGAASMRCGLCSYEFDPAQLTCHGACPLGAHCLVVCCP
                     RCGYSTVNPARSPLAQRLVGALRLGTRAARAGARGAGVVVGDGGAAIRLLDLQPGQAG
                     HVADVAEAEDTLIQLSQYGLLPGTPIRLARKYPVPIIQVGQTDLALDRTLTARIYVDL
                     "
     gene            complement(4127..6088)
                     /gene="feoB"
                     /locus_tag="VF276_14685"
     CDS             complement(4127..6088)
                     /gene="feoB"
                     /locus_tag="VF276_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEX6608149.1"
                     /translation="MNCHGEVGTVPALGLPQVILVGQPNVGKSVLFKRLTSQYVTVSN
                     YPGTTVEIARGKLALDGQRLDLLDAPGINSLQPLAADESVTRALLLERQARSAVQIAD
                     AKNLSRALFLTVQFAELGIPLVLDLNMIDEAQRRGVRVDSGRLGDLLGIPVVATVATR
                     GHGLDALRRNLLAARVPHLQVTYSPEIEAALADLTALLPPELAGKRWHALALLTGDPQ
                     VEHDLHLNPAGCAAVERIRGNAQAGQRRSLRQLVTQQRWQAIAALAAQVYTQDPAATR
                     RPAWGERLAWWAVHPIWGWPILGLVLWLLYKFVGELGAGTLVGFVEKTVFGTVINPAL
                     DRLLMLLAVPALLREVLLGPYGLVTVGLSYGLGIVLPIVTTFFLAFGVLEDSGYLPRL
                     AVLLNRAFQRIGLNGKAVLPMVLGLGCVTMATLTTRILGSRKERVLATLLLALSVPCS
                     AQLGVVLAMVGWLNPGGVVIWAGVVLSVLLLVGGLAARLVPGDSSEFVMELPPLRLPQ
                     LDNLVLKTLARIEWYLKEVIPLFLVAAGGLFVLAKTGVLDGIEAALRPLVTGWLGLPP
                     ETARFFLVGFLRRDYGATGLFDMARQGLLSPNQILVSMVVLTLFVPCVATVLVMLKEH
                     GPKTAGTMVAFVFPFAFLVGGLVRMLVGG"
     gene            6362..6550
                     /locus_tag="VF276_14690"
                     /pseudo
     CDS             6362..6550
                     /locus_tag="VF276_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801227.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(6671..7450)
                     /locus_tag="VF276_14695"
     CDS             complement(6671..7450)
                     /locus_tag="VF276_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012693397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6608150.1"
                     /translation="MGMLDNQVAFITGAASGIGTATARRFAAEGAPVALADVQPEEGE
                     RLRRELDAAGHQTIYVDCDVSNAEAVQQAIETTVERFGKLDIVFANAGINGVWAPVEE
                     FQPDEWEKTLDINLKGTFLTVHFAVPHLKHNGGGSIITSSVNGTRTFSNPGTSAYSSS
                     KAGQVAFMKIMALELGRHNIRCTAVCPGFIYTNIQQRTEERDTDKIGLEVELPQGSPA
                     IDEGPGHPDEVADVCVFLASELGRHVSGVEIYVDGGASLLR"
     gene            complement(7488..7880)
                     /locus_tag="VF276_14700"
                     /pseudo
     CDS             complement(7488..7880)
                     /locus_tag="VF276_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874481.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
     gene            7948..8766
                     /locus_tag="VF276_14705"
     CDS             7948..8766
                     /locus_tag="VF276_14705"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6608151.1"
                     /translation="MLGVPNGNIFQQYLDYLNSLIHLNFGLSYSYFPYSVMHMIGQAM
                     WWTIILVLTTQVIGFIVGNTLGAFAAWRRNSAFDTFVSMGLSFVGTLPFFWIAMLMLF
                     IFAFKLQWLPMGGGFSETSTREFSAGYVGDILKHAILPAAAILLTAPIGWVMGMRNNM
                     IQELGADYTRLAIAKGLKPWKIALLYSARNAILPNVTGFAISLGGIMGGVIFIEGIFD
                     YPGLGRLLFQAVGNRDYPLMQALFLFTTVGVLLANLLADFLYGWLDPRVRKGAT"
     gene            8766..>9499
                     /locus_tag="VF276_14710"
     CDS             8766..>9499
                     /locus_tag="VF276_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6608152.1"
                     /translation="MNTRKSPPLEIESQEAIQADVPSPGASTIGVSGLTGVTGSIPSD
                     APLPLATREEGTEDESMRDDVGQESTRARFKMPSWFTILWQNKNARVGLVMLAIFILV
                     ALLAPILAPYDPHNSSFLPSDQPSAEHLLGTTQAGQDVLSQLIYGARTSLLVGAVAGL
                     LATIIALVIGMTAGYMQGIVDDVLSFLINLGLVVPALPLMISIAAYSPVRGVAIIVFV
                     IALTGWAWGARFKRAQVISLRTRDYIT"
BASE COUNT         1542 a         3210 c         3171 g         1576 t
ORIGIN      
        1 cagggcctgc ggacggttgc tccgtccggg tcatgacacg aaagcgctcc tgtgctatac
       61 tcagcgctgt cgaatgtgcg ggagggacaa agacatggaa cacggatctg cgggaccggt
      121 gcagggtgcg ccggtctatg aaattcgggt gcgcggccac ctcagtccgc agctcgcggc
      181 ctggtttgac gacctgacgg tgacgaatca gccgcaggga gaagcggtcc tggctggggt
      241 gctcagcgat caggcggcgc tctatggcgt gctgttcaag cttaataacc tggggctgac
      301 gctgctggcc gttacgcccc tagcggtgcc ccccgccggc gtcccggacg gcgcgcccta
      361 atcgatccgc ggccgcgggc cctcgccgcg cggcatagga tttgccggag gtggatcggc
      421 cgccacccgt gccactcgta ggggcgcgat tggcgcccca gtcgggggcc ggtatcccgg
      481 cgcgcggcgg cacctggtcc cgatccggca gcaccagggg gtgttatggc tgcaccggcc
      541 acgcccgggc cacccgcaga cggccccgcc cggctcctgc ccacgaaaat ccggattccc
      601 tcgatgactc cggggctcgt gccccgcccg cgcctgcgcg aactgctcca gcaagggctg
      661 cagcgccgct tgctgctgct ctccgcaccc gccggcttcg gcaaaaccac gctgctggcc
      721 gcgtggattc acgagtcggt ggccccgacc atgcccgtgg cctgggtcac gctagatgcg
      781 ggggataatg atcctctgcg cctcggccgc tatctgtgcg ccgcgctcaa tcaggcgcag
      841 gcggggttgg ccccccagac cacccgcctg ctccaggccc cccacccacc cgctctggag
      901 gcgatcctga cggagctggt ccacgaactc gccgcccggt cgcagccgct ggtgctcgtg
      961 ctcgacgact accaggcgct caccgcgccc gccattcccc ccttgctcac cttcctgctc
     1021 gaccaactcc ccgacaccgt gcacctcgtg ctcgccagcc ggatcgatcc gctcctgccg
     1081 ctcgcgcgcc tgcgcgtcca gcgccagctc gtggagatcc gggcgggcga cctacgcttt
     1141 acggcggacg aagcgaccgc ctttctcaat aagttcatgg ggttggcttt ggttccgacg
     1201 gctatcaccc tgctggccgc ccgaacggaa ggctggatcg ccgggcttca gctggctgcc
     1261 ctgtccctgc agggtcgcac cccggccggg atcgccgcct ttctcgccgc cttcgccggt
     1321 agtcaccact tcatcctcga ttacctggga gacgaggtgc tgcaccggca gccgccggcc
     1381 acccagcagt tcttgctgca aaccgccatc ctcgaccgcc tcagcgcctc tctgtgtgat
     1441 gcggtcaccg agagcacggg gagccaggcc atgctggagc agttagagca gcgcaacttg
     1501 ttcctgattc ccttggacga ggagcggcac tggtatcgct accatcagtt gtttgccgaa
     1561 tttctgcgca gccggctcgc ggcggcgggc accctggacg tgccggtcgc cgccctgcat
     1621 cagcgcgcgg cgcgctggta tgagcgccac gcgttcctgg accaggcggt cgagcacgcc
     1681 ctggcggcgg aggacttcgc cggggcggcc cggctcgtcg aacagtatct cgtctccggg
     1741 ttccggggcg gggaactcga gacgctgctg cgctggttta cggccctccc cgcggcaacc
     1801 acgcgcccgc gcgcccggct cagtctctat tacgcgcagg tgctgctgtt gagcggccgg
     1861 tgggcggcgg tcccccactg gctgcaggcg gccgaacaga gtctggccgc gcagggcgcg
     1921 agctggccgg aaggggagcg gcgcacggtc cagggcgagg tgagtgccat ccgtgcgctg
     1981 ttcgcctcct tgcacgccga ggtcgcccag gtcgatcggc tggcggacga cgcccgccgc
     2041 ctgttacctc ccgaccatcc gctgtgggcg ccgctgttgc tgagtcaggg gagcgcgtac
     2101 tggttagccg gtgacctggc ggcggcggtc ggggtattga cccaggccgg cgaggcggcc
     2161 cagcgcacgg gcaacatgta cgtgttctac atcatcaccg tctacctcgc gcaggtgcgg
     2221 ctggcccagg gccacttgcg tgaagcgatg cggctccagg agagcgggcg gcagttggtc
     2281 acggcgcggg ggcgcgaagg agccgaaggc aacggcttgg cggtcgggtt gggggcgctg
     2341 tggtgtgaag gccaggcgct agcggaggcc caggcgctga ttgagcaggg actggagcgg
     2401 gcccagcagg agcgcaacgg gattgtggtc atcgggggca aagcgaccct ggcctgggtg
     2461 cggcacgccc agggccaggc gcagcaggcg gaggcgctga tgcaggaggc ggtcacgctg
     2521 ggccgccagc agggcgtcga ctggacctgg atcaccggcc cgctgcgcac ggcgcaggcc
     2581 cgcctgtggc tggcccaggg caacctcacg gccgccgcgc aatgggcggc ggagccggcc
     2641 ggagaggccc ccgtgccggc gtattttgcg gaagccgccg cgatcctgca ggcccgcctc
     2701 tatcgggccc aggggcggta cggggatgcg ctggcgctgc tggatcggct gcaaggccca
     2761 gtgactgcgg ccggccaccg gcagcactta ctggagatcc tggtgctgcg ggcgggcctc
     2821 gcgcaggacc agggccagca gggggcggcc cgcacgaccc tgcgcgccgc ggtggatctg
     2881 gccgcgccgg agggctttgt gcgcaccttc gtggaggggg ggcccgcgct ggcggaactg
     2941 ttgcagagcc tgcgcccggt gcgagccgag caggcgacct acctggcgcg cgtgctaagg
     3001 gcgttcgcgc cggaagccgc tccggggccg gcggcccccc caccgccggg cctgcggagc
     3061 gacgatgcgc tgagtgagcg ggaactggac gtgctgcgcc tgatggcggg gggcgcgtcg
     3121 aatcaggaaa ttgcggcgga gctggtgatc gcggtcaata cggtgaagcg gcatgcgcgc
     3181 aatatttttg aaaagctcga tgccgccaat cggacacagg cggtggcccg cgcgcgggac
     3241 ctgggcctgc tcggcccccg ggattccccg cgctgagcgg ccggggctgg cccggcgggc
     3301 gcggtcgacg cgagaggagg tggcgcaggc ggtggcgaat ccgctccggc gcggtgcacg
     3361 cggcctcgct agtcgcgcgg ggggtctccg gcacatcgcg ctggggtgcg gagtacatag
     3421 tagcagtaac gatccgctca caccccgcct gccgctagac atctggctac agtgccgggg
     3481 ccgctcctgc ccgacccgct ggcgttcctg ccggcgctcg tgggggtgct gctcgcgctg
     3541 gtgaccggct ggctgggcgg cgagttagtc gaccggcgcg aggtgggtgt ggacgcgggg
     3601 accaacctga acgcccccag ttcgcaaacg atctgtgtca cagatcgaca taaatacgcg
     3661 ccgtcagcgt gcggtccagc gccagatcgg tctgaccaac ctggataatc ggcaccgggt
     3721 atttgcgcgc tagccgaatc ggggtgccgg gcagcaggcc gtactgactc aactggatca
     3781 gcgtgtcttc tgcctctgcc acgtcggcga catgcccggc ctgccccggc tgcaggtcca
     3841 gcaagcggat ggcggcgcca ccgtccccta ccacgacgcc ggccccgcgg gcgccggccc
     3901 gcgcggccct ggtcccgagc cgcaaggcac cgaccagacg ctgcgccagc ggggatcggg
     3961 ccggattcac cgtggaatat ccgcagcgcg ggcaacagac caccaggcag tgcgcgccca
     4021 gcgggcaggc gccatggcag gtcagttggg cggggtcgaa ctcgtagctg cacagcccgc
     4081 aacgcatgga tgccgcaccg gccgccgatg aaaccgccat cgattgctac cctcccacca
     4141 gcatgcggac caggccccca accaggaagg cgaacggaaa cacgaaggcg accatcgtgc
     4201 ccgccgtctt ggggccatgc tccttcagca ttaccagcac ggtggccacg caaggcacaa
     4261 agagcgtcag cacgaccatg ctcaccagga tctggttcgg ggacaggagt ccctgccgcg
     4321 ccatatcgaa caggcctgtg gccccatagt cgcgccgtaa gaacccgacc aggaagaagc
     4381 gcgctgtctc cggcggcagc ccgagccagc ccgtcaccag gggtcgcagc gcggcttcga
     4441 tcccatcgag tacgcccgtt ttagccagca cgaacagccc gccggcggcg accaggaaga
     4501 gcgggatcac ctctttgaga taccactcga tgcgggccag cgttttgagc acgaggttgt
     4561 cgagttgtgg cagccgcaac ggcggcaact ccatgacgaa ctcggagcta tcccccggca
     4621 cgagccgcgc agctagcccg cccaccaaca gcaggacgct cagtaccacc cccgcccaga
     4681 tgaccacgcc acccggattg agccacccga ccatcgccag caccaccccg agttgcgccg
     4741 aacagggcac gctcaacgcc agcagcagcg ttgccagtac ccgctccttg cggctgccca
     4801 agatccgcgt ggtcagggtc gccatcgtca cgcagccgag ccccaggacc atcggcagga
     4861 ccgccttccc gttcaggccg atccgttgga aggcgcggtt gagcagtacc gccagccggg
     4921 gcaggtagcc ggagtcctcc agcacgccaa aggccagaaa gaaggtcgtg acgatcggca
     4981 gcacgatgcc aaggccatag ctgagaccca cggtgaccag cccatagggt cccagcaaga
     5041 cctcgcgcag cagggccggg accgccagca gcatcaggag gcggtcgagg gccgggttga
     5101 tcacggttcc aaagaccgtc ttctccacga agccgacgag cgtgcccgcg cccagttcgc
     5161 ccacgaactt gtacagcagc cagagcacca gccccaggat cggccagccc cagatcgggt
     5221 ggaccgccca ccaggccagc cgctcgcccc acgcggggcg tcgcgtggcc gcggggtcct
     5281 gcgtgtagac ctgggcggcg agggcggcga tcgcctgcca gcgctgctgg gtcacgagct
     5341 ggcgcagcga ccggcgctgg cccgcctggg cgttaccccg gatgcgctcg acggccgcgc
     5401 agccggctgg gttcaggtgt agatcgtgct cgacctgcgg atccccggtc agcaaggcca
     5461 gggcgtgcca gcgtttacct gccagctcgg gtggcaagag ggcggtcagg tcggccaggg
     5521 cggcttcgat ttccgggctg taggtgacct gcaggtgcgg cacgcgggcg gcgagcaggt
     5581 tgcgccgcag cgcgtccagc ccatggccgc gggtggccac cgtcgccacc accgggatcc
     5641 caagcaggtc gcccagccgc ccggaatcga cccgcacccc gcggcgctgg gcctcgtcga
     5701 tcatgttcag atcgagcacg agcggtatgc cgagttcggc gaactgcacg gtcaggaaca
     5761 gcgcgcggga gaggttttta gcatcggcga tctgcacggc gctccgcgcc tgccgctcca
     5821 ggagcagggc acgggtgacg gactcgtcgg ccgccagcgg ttggagactg ttgataccag
     5881 gcgcatccag caggtccagc cgctgcccgt ccagcgccag cttcccgcgg gcgatttcga
     5941 cggttgtccc aggatagttg gagacggtaa catactggct cgtaagccgc ttgaagagta
     6001 ccgatttgcc cacattaggt tgccccacca atatgacctg gggcagcccc aatgccggaa
     6061 cggtacccac ctcgccgtga cagttcattt agtcccgatc cttagtctcg ccgtacatct
     6121 cctcaccaac cgtgataagc agcgggttag agcgccgcga atcccggcgc cggcgcccgc
     6181 tgcgtagcac cgagagcata gtacacgcgg gaggtgagaa gcagggtata cgcaagtgaa
     6241 tagggtgtaa aacctggaag ggacgcctgt agcatcgcgt ggttggtgag acggtctcaa
     6301 agatcgtggg actagccggg tagggcttgc gcccattcta tcatgagtgt cggatcaagt
     6361 cttgactatt acaaataaac tcggggtgct ccgcctggcc gagcaactgg gcaacgagag
     6421 taaagcttgt cagatcatgg actactcccg cgacagcttt tatcgcttga aagagttgta
     6481 tgcggcaggt ggcgaggcgg tgctggccgg catcagccgc aagaaaccct tgcccgccaa
     6541 tcgggtcgct gccgcctcag aatatggtga cggtggttga gcgaacacgc gacgcagttg
     6601 ctgcgcggcg cctacctagc gacgcacttc ccagaactgg gtcgcattct gaatacacat
     6661 cctcgtatcg tcaacgcagc aacgaggcgc cgccatcgac gtagatctcc acgccggaga
     6721 catggcgccc cagttctgat gcgaggaaca cacagacatc ggccacctcg tccgggtgcc
     6781 cggggccttc gtcgattgcg ggactgccct gcgggagttc gacttcgagt ccgattttgt
     6841 cggtgtcgcg ctcttcggtg cgctgctgga tgttcgtgta gatgaagccg gggcagacgg
     6901 cggtgcagcg gatgttgtgc cgcccgagtt ccagcgccat gatcttcata aaggcgactt
     6961 gtccggcctt ggagctgctg taggcgctcg tgccggggtt cgagaaggtg cgtgtgccgt
     7021 taacgctgct cgtgatgatg ctgccgccgc cgttgtgctt caggtgcggc accgcgaaat
     7081 gcacggtcag gaaggtgcct ttgagattga tgtccagcgt cttttcccat tcgtcgggct
     7141 ggaactcctc gacgggtgcc cagacgccgt tgatcccggc gttggcaaag acaatatcca
     7201 gcttgccgaa ccgctccacg gtcgtttcga tggcctgttg gacggcctcg gcgttgctca
     7261 cgtcgcagtc cacatagatc gtctggtggc ccgctgcgtc cagttcgcgg cggaggcgct
     7321 cgccctcttc aggctgtacg tcagcgaggg ccaccggcgc cccttctgcg gcgaagcgcc
     7381 gggcagtggc cgtgccgatc ccgctggccg cgccggtgat gaaggcaacc tgattgtcca
     7441 gcatgcccat gtgaccgtct tcctttctgc ggcacagccc cggccggcta taaagcctcg
     7501 cgatggcgtg cggcaagccg tgctgcgtga tccccgcgcc ctgtcgtccg caggcgctcc
     7561 aggtagcgcg gcaacatgcc ccgtgtgtgg aagggccctc cttcccgcaa cacggtcatc
     7621 aacgggtcta catccgtccc gctctccagt atcatcgcat gctgccagcc ggcgagcaag
     7681 gccattgccc tgtctaccac ctcggggcga tcggctgcca ggtcatgctg ctcatgcggg
     7741 tcgtgtgcca ggtcgaacag catgatcggc tccagttcct tatacccatc atgataggtg
     7801 cgcaagcaca ggtaatcgtc aaaacgcacc ccgcgctggc acgcccacgc gccctggctg
     7861 gtgatgagat acggccggcc cgcctcccgc ccttcgcgga acgcggccgt gaacggctgg
     7921 gcgtcccagt cgcaggccat ccggacgatg ctgggcgtgc cgaacggcaa catcttccag
     7981 cagtacctcg actatctaaa cagcctcatc cacctcaatt tcggcctgtc ctacagctac
     8041 ttcccctaca gtgtcatgca catgatcggg caggcgatgt ggtggaccat catcctcgtc
     8101 ctgaccacgc aggtgatcgg gttcatcgtg ggcaacaccc tgggggcgtt cgcggcctgg
     8161 cgccgcaaca gcgccttcga tacctttgtt agcatgggct taagtttcgt cggcactctg
     8221 ccgttcttct ggattgccat gctgatgctc ttcattttcg cctttaagct ccaatggctg
     8281 cccatgggcg gtggcttcag cgagacatcc acgcgggagt ttagcgccgg ctatgtcggc
     8341 gacatcctca agcacgccat cctgccggcg gcggcgatcc tgctcacggc gcccatcggc
     8401 tgggtgatgg gcatgcgcaa caacatgatt caggaactgg gcgcggatta cacccgcctg
     8461 gccatcgcca aagggttaaa gccctggaag atcgcgctac tctacagcgc ccgcaacgcc
     8521 attctgccga acgtcaccgg cttcgccatc tcgctcggcg gcatcatggg cggcgtgatc
     8581 tttatcgaag gcatcttcga ctatccgggt ctgggccggc tgctgttcca ggccgtgggc
     8641 aaccgcgact acccgctaat gcaggccctc ttcctgttca ccaccgtcgg cgtcttgctg
     8701 gcgaacctgc tcgctgactt cctgtatggc tggcttgatc cacgggttcg gaaaggagcg
     8761 acatgatgaa tacccggaaa tctccaccgc tggaaatcga gtcccaggaa gcgatccagg
     8821 cggacgtgcc ctcgcccggt gccagtacca tcggcgtgag cgggctgacc ggcgtcacgg
     8881 ggtcgattcc gagcgatgcg cccctaccgc tggcaacccg cgaggaagga accgaagacg
     8941 agagcatgcg ggacgacgtg gggcaggagt ccacccgcgc ccggttcaag atgccgtcct
     9001 ggttcacgat tctatggcag aacaagaacg cgcgggtcgg gttggtgatg ctcgccatct
     9061 tcatcctggt cgcgctgctg gcgccgatcc tggcgcccta tgacccgcat aacagcagct
     9121 tcctgccgtc cgatcagccg agcgccgagc acctgctggg caccacgcaa gcgggccagg
     9181 acgtgctgtc acaactgatc tacggcgcgc ggacctcgct gctcgtcggc gcggtggccg
     9241 ggttgctggc gaccatcatc gccctggtca tcggcatgac ggccggctat atgcagggca
     9301 tcgtcgacga cgtgctctcg ttcctcatca acctgggcct ggtagtgccc gcgctcccgt
     9361 tgatgatctc gattgccgcc tattcgccgg tgcggggcgt ggcgatcatt gtctttgtca
     9421 ttgctctcac cggttgggcc tggggtgcac ggttcaaacg cgcccaggtg atctcgctgc
     9481 gcacacgcga ctacatcac
//
LOCUS       DASUYC010000899         3087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_585040,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000899 DASUYC010000000
VERSION     DASUYC010000899.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585040"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VF276_14715"
     CDS             complement(<1..292)
                     /locus_tag="VF276_14715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6608153.1"
                     /translation="MENYHWRPVQPADRAALADLDAACRAEDGPVSVADPSYETLLGT
                     PHTALLCGTREGNPQIVAVGWVQVNGAQARLGGKVHPALRRQGLGTHLLRWSE"
     gene            complement(354..1916)
                     /locus_tag="VF276_14720"
     CDS             complement(354..1916)
                     /locus_tag="VF276_14720"
                     /inference="COORDINATES: protein motif:HMM:NF022466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HEX6608154.1"
                     /translation="MCLLASCLADELLPAAAALAEPPAPLLAEALPLQEKTLAAIWNA
                     QRPLQGPFWTTAHEPVAIIYRGHWAGGAGPDFRGAIVAFGDNRPQRGDIELHLRAADW
                     YAHGHHTDPAYNDVLLHVVYSLGSGADGPARLADARRARTAAGREVPTLVLAPHILAD
                     AATLAALDLPAQLGDLSEEPCWQRTAHRPLSEVLDVLGAAGDARFAEKAARYEADLAV
                     ALDGLPAHEHEAAAEQILYAGLCDGLGYSANRAPFAALAARLPLRLLHLIAGDRPTAD
                     RTVVLEAVLFGGAGLLASQRKLTRTLDWQSAAQADELERQWAALRPLLGHVLADGTAS
                     LGWKFAGVRPANAPARRVAALAHLLSWMLPGGLLPGFLTDAARDHAPADLAARWLARL
                     HVAAGGSFWSEYSDFGARLGGAGSTADLIGQDRAGELLVNVVLPFLAAWGAHTDQPTL
                     TATARAVYAAHPRLADNQVTRTMLGEVLGPRARQARLGAREQQGLIGLHRRYCSVRDI
                     YECPLSGLRRPV"
     gene            2240..2998
                     /locus_tag="VF276_14725"
     CDS             2240..2998
                     /locus_tag="VF276_14725"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX6608155.1"
                     /translation="MRMPRYDVRNDGVGPYAVFYCDKCYREYRSQPDIQNTITKDVGR
                     AALGGLLRNIPVVGYSVANNVLEDPRYITSLKPDQLEAAWQQVQENFHECPTCRLIVC
                     PSDWDPQAGFCTEDSPRRAEIAQAQAEQAAGVVKGFAAAFGLGQVVSDLGAAAKAAAA
                     RTAHCSQCGTLAAPGTKFCAKCGGAMIQPEPVAAPVTCPTCQAAVPPGMAFCTNCGTK
                     VVATAPAAPQPTTCPTCGAKVAGRFCGACGSKVS"
BASE COUNT          452 a         1097 c         1118 g          420 t
ORIGIN      
        1 cttcgctcca gcgcagcagg tgggtgccca ggccctggcg gcgcagcgcg gggtggacct
       61 tgccgcccag ccgtgcctgc gccccgttca cctgcaccca tcccacggcc acgatctgcg
      121 ggttcccctc ccgcgtgccg cacaatagcg ccgtatgcgg cgtgcccaac agcgtctcat
      181 agcttgggtc ggcgaccgag acggggccat cctccgcccg gcacgccgca tccagatcgg
      241 ccagggctgc ccgatcggcg ggctgtacgg ggcgccagtg gtagttttcc atggtcatgc
      301 tcctatcgta gatgtggata ggagtatacc agataccggc ggaggcaggc cagttacacg
      361 ggacggcgca ggcccgacag cgggcattcg tagatgtcgc ggacagagca gtagcgacgg
      421 tgcaggccga tgaggccctg ctgctcgcgg gcgcccaggc gggcctggcg ggcgcgcggg
      481 ccgagcactt cgcccagcat ggtgcgcgtc acctggttat cggcgaggcg cgggtgcgcg
      541 gcgtagacgg cgcgggccgt ggcggtgagc gtgggttggt cggtgtgggc gccccaggcg
      601 gccaggaagg gcagcaccac gttgaccagc aactcgccgg cgcgatcctg gccgatcagg
      661 tccgcggtgc tgcccgcgcc gcccaggcgc gcgccgaagt cgctgtattc actccagaac
      721 gagccacccg ccgcgacgtg cagtcgggcc agccaccgtg cggccaggtc cgcgggcgcg
      781 tggtcgcgcg cggcgtcggt caggaagccc ggcagcaggc cgccgggcag catccagctc
      841 agcaagtggg ccagggcggc cacgcgccgg gccggggcgt tggccgggcg cacccccgcg
      901 aacttccagc ccaggctcgc cgtgccgtcg gccagtacat ggcccagcag cgggcgcaag
      961 gcggcccact ggcgctccaa ctcgtcggcc tgcgcggcgc tctgccagtc gagagtccgg
     1021 gtcagcttgc gctgcgaggc cagcagtccg gcgccgccga acagcaccgc ctccagcacc
     1081 accgtccggt cggcggtggg gcgatccccg gcgatcaggt gcagcaggcg cagcggcagg
     1141 cgcgccgcca gggcggcgaa gggcgcgcga ttggcgctat agcccagccc gtcgcagagg
     1201 ccggcgtaga ggatctgctc cgccgctgct tcgtgttcgt gcgcgggcag gccgtccagg
     1261 gccacggcca ggtcggcctc gtagcgcgcc gctttctcgg cgaagcgggc gtccccggcg
     1321 gcgcccagca cgtccaatac ctcgctcagc gggcggtggg cggtgcgctg ccagcacggc
     1381 tcctcggaca ggtcgcccag ttgcgccggg aggtcgagcg cggccagggt ggcggcgtcg
     1441 gcgagaatat gcggcgccag caccagcgtc ggcacctcgc gcccggcagc ggtgcgtgcc
     1501 cggcgggcgt cggccaggcg ggccgggccg tccgcgccgc tgcccaggct gtagaccacg
     1561 tgcagcaaca cgtcgttgta ggccgggtcg gtgtggtggc cgtgggcgta ccagtcggcg
     1621 gcgcgcaggt ggagttcgat gtcgccgcgc tggggccggt tgtcgccgaa ggccacgatg
     1681 gcgccgcgaa agtcgggccc ggccccgccc gcccaatgtc cgcggtagat aatcgccacc
     1741 ggctcgtgcg ccgtcgtcca gaacgggcct tgcagcgggc gctgcgcgtt ccagatcgcc
     1801 gccagcgtct tctcctgcaa cggcagcgcc tcggccagca ggggcgcggg tggctcggcc
     1861 agggccgccg cagcgggaag caattcgtcg gcgaggcagg acgcgagcaa gcacatgagc
     1921 ggatctcctc gtatgcaacg gcgggtgggt ctggacgcgg agtatagcac aagcgagcaa
     1981 gctccgcaac aagctgacac ggctcgccgg cataaggcca actggcgatt gctacagttt
     2041 cgtaactctt gcgcccgcta attgacgctt ttgcgcgctt agcctatact caagatagat
     2101 gtgcccgtcc ggtgccgatc ccgacgacac gagacacgcg tagggcgggg tcggcgcgct
     2161 gttacacagg caaccactac gatctacacc atccgcgatc cgcctgcgtt ggcgtcccct
     2221 ggaaaaggag aacccgttca tgcgaatgcc ccgttacgac gtgcgcaacg acggcgtcgg
     2281 tccctatgcc gtcttctatt gtgataagtg ctaccgcgaa taccgcagcc agcccgacat
     2341 ccagaacacc atcaccaagg acgtgggccg ggcggcgctc ggcgggctgc tgcgcaacat
     2401 cccggtggtc ggctactcgg tcgccaacaa cgtgctggaa gacccgcgct acatcaccag
     2461 cctgaagccc gaccaactgg aggccgcttg gcagcaggtg caggaaaact tccacgagtg
     2521 ccccacctgc cgcctgattg tctgccccag cgactgggac ccgcaggcgg gcttctgcac
     2581 cgaggacagc ccccgccgcg ccgagatcgc ccaggcgcag gccgagcagg ccgccggggt
     2641 ggtcaagggc tttgccgccg ccttcgggct gggccaggtg gtcagcgacc tgggcgcggc
     2701 ggcgaaggcg gcggccgcgc gcacggccca ttgctcgcag tgcggcacgc tcgcggcgcc
     2761 gggcaccaag ttctgcgcga agtgcggcgg ggcgatgatc cagccggagc cggtggcggc
     2821 ccccgtcacc tgccccacct gccaggccgc cgtgccgccg ggcatggcct tctgcaccaa
     2881 ctgcggcacc aaggtcgtcg ccacggcccc cgccgcgccc cagcccacca cctgccccac
     2941 ctgtggcgcg aaggtggccg ggcgcttctg cggcgcctgc ggcagcaagg tgagctagcg
     3001 taaccctggg ctgaacgggc tactgcggca ggccgcgcag gcggtcttcg ttgcggtagc
     3061 ccgccccttg aggggccggg cggcgcc
//
LOCUS       DASUYC010000900         4314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_379397,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000900 DASUYC010000000
VERSION     DASUYC010000900.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379397"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2788
                     /locus_tag="VF276_14730"
     CDS             <1..2788
                     /locus_tag="VF276_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEX6608156.1"
                     /translation="GTYVYILGGITSAYQKVMYRYDPVANTYTQMAPFNTGVWAAAVA
                     YQNGKIYKIGGNISSGQTNAVEVYTISTNTWAAAANLPAALTFASAIGYNGYIYVAGG
                     SAASASLKTYRYDPVANSWNDAAIADLPATRWGAASDLYNGQWILAGGVAGGSLSSTV
                     VALDMTNPTGAWATLPSMLQPVYRLGGATAGAAFYAVGGRNDGTFTGTNFNQRYLPLT
                     CATATPTFTTTSTATPTDTPTNTATPTATPTNTATPTDTPTNTATPTATPTNTATPTD
                     TPTNTATPTATPTNSPTPTDTPTPTDTPTATPTNTPTATNTPTNTPTYTATATDTPTN
                     TPTSTATDTPTSTPTDTPTATNTPTATPTNTATPTDTPTPTDTPTNTATPTDTPTNTP
                     TPTDTPTNTATPTDTPTYTATPTDTPTPTNTPTDTPTYTATATNTPTLTATATNTPTY
                     TATATNTPTDTPTNTATPTDTPTYTATPTRTPTSTATPTNTPTDTPTDTPTSTPVPPT
                     NTPTNTPTNTAVPPTATATNTPTNTAVPPTNTATNTPTNTAVPATSTPTNTPTNTAVP
                     PTATPTRTAMPLTYTPTTGNATATATATNTPTNTAVPPTATPTNTPTYTATPINTPTA
                     TATHTAIPTVTPTNTPTNTAVPPTSTPTNTPTDTPVVPTSTPTDTPVSAGTATPTATT
                     VPNSTATATPVSTATGTATATVTATSTQTAQPATTGTATAIPATATVTPICSLSFSDV
                     QEEYWAFGYIKWTYCQGIISGYSDGTFRPEANTTRGQLAKMVVLAAGLPLVLPAGAPH
                     FSDVPPGQPFYRYIEVAWSHNIITGYEDGTFQPNAPVTRAQLAKMIVQARGLSLVDPL
                     VARFRDVPRDSWAYRYVETAAAHQIVGGYDCGAAGEACPGFYYRPDVPATRAQLTKLL
                     HQAFAVPSLDQPQP"
     gene            2998..>4314
                     /locus_tag="VF276_14735"
     CDS             2998..>4314
                     /locus_tag="VF276_14735"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX6608157.1"
                     /translation="MRGAFCPHCGHPARPGARFCAGCGSLLAAGAGVLPVGTTLQSGR
                     YTVLGLMGRGGNGAVYQAEDTRLRRACVVKELQPTFLSTAEQRRAEADFHREALILAR
                     LSTEHPGLPQTYDFFNEQGRHYLVMQYVAGLDLESRLRTGGPLAEAEAVGHGAAIAEV
                     LAFLHAQQPEPVVHRDVKPANIIVDAQGRVKLVDFGLAKALLSTTSALRAGTAGQTSA
                     AGTAGYTPLEQWALAAEARSDVYALGATLHHLLTGRDPRTVFEGQGELNLDLIRRLTV
                     FPPLRALRGDVAPALDSLLAAMLASEPTARPPAAEVSATLSGLLKPGRSRVTSRPARR
                     PAAAPPEPAVLSVPLLDRPRLAAAVAGWLRAHVEGFPAGEPVLVESARADLVPLALGA
                     YHVAARYEAPDGRVIHSLDERGVAVLDGATARPLDPPLAQFLAGMRG"
BASE COUNT          784 a         1688 c         1338 g          504 t
ORIGIN      
        1 cggtacctat gtctatatcc tcggcgggat tacgtccgcc taccaaaagg tgatgtatcg
       61 ctacgatccc gtcgccaaca cctataccca gatggccccg ttcaacacgg gcgtctgggc
      121 ggcggcggtg gcgtaccaga acggcaagat ctacaagatc ggcggtaata tcagtagcgg
      181 gcagacgaac gccgtcgagg tctacacgat cagcaccaac acctgggccg ctgccgcgaa
      241 cctgcccgct gcgttaacgt tcgccagcgc catcggctac aacggctaca tctacgtggc
      301 cggaggtagc gcggcctctg cgtcgctcaa gacctatcgc tacgaccccg tggcaaatag
      361 ctggaacgac gcggccattg ccgatttgcc cgccacgcgc tggggggctg cttcggacct
      421 ctacaacggc caatggatcc tggccggtgg cgtggcgggc ggatccctct ccagcaccgt
      481 ggtggcgctc gatatgacca acccaacggg cgcctgggcg acgctgccgt cgatgcttca
      541 gccggtttac cggctgggag gggccacggc cggcgccgcc ttctatgccg tcggcgggcg
      601 caacgacggc accttcaccg gcacgaactt caaccagcgc tacctgcccc tcacctgcgc
      661 cacggccacg ccgacgttca ccaccaccag cacggcgacg cccacggaca cgcccacgaa
      721 cacggcgacc ccgacggcca cgccgaccaa tacggcgacg cccacggaca cgcccacgaa
      781 cacggcgacc ccgacggcca cgcccacgaa tacggctacc ccgacggata ccccgaccaa
      841 cacggctacg ccgacggcta cgccgaccaa ctcgcccacg ccaactgaca cgccgacgcc
      901 caccgacacg ccgaccgcca caccgacgaa tacgccgacg gcaactaaca cgccgacgaa
      961 tacgccaacc tacacggcaa cggccaccga taccccgacc aatacgccga caagtaccgc
     1021 caccgatacg ccgacaagta cccccacgga tacgccgacg gcgactaaca ccccaacggc
     1081 gacgccgacc aacacggcca ccccgacgga cacgcctacg cccaccgaca cgccgacgaa
     1141 cacggccacg ccgacggaca cgccgacgaa tacgccgact ccaaccgata ccccgaccaa
     1201 tacggcgacg ccgacggata ccccgacgta caccgcgacg ccgaccgata cccccactcc
     1261 gacgaatacg ccgaccgaca cgccgacgta cacggcgacg gcgaccaaca cgcctaccct
     1321 cacggcgacc gctacgaata cgccgacgta cacggccacg gccaccaaca cgccgaccga
     1381 tacgcccacg aacacggcga cgccgacgga taccccgacg tacacggcca ccccgactcg
     1441 tacgccaaca tcgacggcga ccccgacgaa cacaccgacg gatacgccaa ccgatacgcc
     1501 gacgagcacg ccggttccgc cgaccaatac gccgactaat accccgacta atacggcggt
     1561 gccgccgacg gctacggcta cgaatacgcc aaccaacacg gcggtgccgc cgacaaacac
     1621 ggcgacgaac acgccgacta ataccgcagt gccggccacc agcactccta ccaacacccc
     1681 gaccaatacc gcggtgccgc cgacggctac gccgacgcgg acggccatgc cgctgaccta
     1741 taccccgacc acgggtaatg cgacggcgac cgcgacggcc accaacacgc cgaccaatac
     1801 agccgtgccg ccgacggcga cgccgaccaa cacgccgacg tacacggcga cgccgatcaa
     1861 cacgccgacg gctacggcga cccatacggc catacctacg gtcacaccga cgaacacgcc
     1921 gacgaacacc gccgtgccgc cgacgagcac gccgacgaat accccgaccg atacgccggt
     1981 cgtgccgacg agcacgccca ccgatacgcc ggtcagcgcg ggcacggcga caccgaccgc
     2041 taccaccgtg ccgaacagca cggccacagc cacgccggtg agtacggcca cgggcaccgc
     2101 aaccgccacg gtcacggcta catcgacgca gaccgcgcaa ccggcgacca ccgggacggc
     2161 gacggcgatc cccgcgacgg ccacggtcac cccgatctgc tcgttgtcgt tcagcgacgt
     2221 gcaggaggaa tactgggcct tcggctatat caagtggacc tactgccagg gcatcatcag
     2281 cggctatagc gacgggacgt tccggcctga ggccaacacg acccgcggcc aacttgccaa
     2341 gatggtggtc ctcgcggccg ggttgccgct ggtgctgccc gccggggcgc cgcacttcag
     2401 cgacgtgccg ccgggccagc ccttctaccg ctacatcgaa gtggcctgga gccacaacat
     2461 catcaccggg tacgaagacg ggaccttcca gcccaacgcc ccggtgacgc gcgcgcaact
     2521 ggcgaagatg atcgtccagg cgcgcgggct gagcctggtc gatccgctgg tcgcccggtt
     2581 ccgggacgtg ccgcgcgact cgtgggccta tcgctacgtc gagacggccg ccgcgcatca
     2641 gatcgtgggc ggctacgact gcggcgcggc aggcgaggcc tgcccaggct tctactaccg
     2701 gcccgacgtg ccggccaccc gcgcccaact gaccaagctg ctgcatcagg cgttcgccgt
     2761 gccgtcgctc gatcagcccc aaccgtaacg ccacccccgc gccgggcgag cctgtgcccg
     2821 cccggcgcac ccaccggcgg gccggaccta tgggtccggc ctgccgcgct ttcggcggcg
     2881 aggcgcacaa gtggttcggt gcccgcgtta catgctataa tggggcgaac ggcggcccgg
     2941 cggtgacacc aggggtcggg cgcctgggaa cttcccctgc tggagaggag cgcccgcatg
     3001 cgtggcgcct tttgccccca ctgtggccac ccggcccggc ccggcgcccg gttctgtgcc
     3061 ggatgtgggt cgttgctggc cgcgggcgcg ggcgtgttgc cggtcggcac gaccctgcaa
     3121 agcgggcgct atacggtcct ggggctcatg ggccggggcg gcaacggcgc ggtctaccag
     3181 gccgaggata cgcgactgcg ccgggcctgc gtggtcaagg aactgcaacc caccttcctg
     3241 agcaccgctg agcagcgccg cgccgaggcc gacttccacc gcgaggcgct catcctggcc
     3301 cggctcagca ccgaacaccc cggcctgccg caaacctacg acttcttcaa cgagcagggc
     3361 cgccactacc tggtgatgca atacgtggcc gggctggacc tggagagccg cctgcgcacc
     3421 ggcgggccgc tggccgaggc tgaggccgtg ggccacggcg cggctatcgc cgaggtgctg
     3481 gccttcctgc acgcccagca accggagccc gtggtccacc gcgacgtgaa gccggcgaac
     3541 atcatcgtcg acgcgcaggg gcgggtgaag ctggtcgact tcgggctggc gaaggcactg
     3601 ctctcgacca caagcgccct gcgcgccggg accgcgggcc agaccagcgc cgcgggcacg
     3661 gcgggctata cgccgctgga acaatgggcg ctggccgccg aggcgcgctc ggatgtctac
     3721 gccctgggcg cgaccctgca ccacctgctg acgggccgcg acccgcgcac cgtcttcgag
     3781 ggccagggcg agttgaacct ggacctgatc cgtcgcctca ccgtgttccc gccactgcgc
     3841 gcgctgcgcg gcgatgtggc gcccgcgctc gacagcctgc tcgcggccat gctcgcatcc
     3901 gaacccacgg cccgcccgcc cgccgccgag gtgagcgcca ccttgagcgg gctgttgaag
     3961 ccgggccgca gccgcgtcac ctcgcgcccg gcgcggcggc ccgcggcggc gccgcccgag
     4021 ccggcggtgc tgtccgtgcc gttgctggat cggccccggc tggcggccgc ggtggccggg
     4081 tggctgcgcg cccacgtcga aggcttcccg gccggggaac ccgtgctggt ggagtcggcg
     4141 cgcgccgacc tggtgccgct ggccctgggc gcctaccatg ttgctgcccg ctacgaggcc
     4201 cccgatgggc gggttatcca ctcgctggac gagcgcggcg tggccgtgct cgacggcgcc
     4261 acagcccgcc cgctcgatcc gccgctggcg cagttcctcg ccgggatgcg cggc
//
LOCUS       DASUYC010000901         2000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1073069, whole genome shotgun sequence.
ACCESSION   DASUYC010000901 DASUYC010000000
VERSION     DASUYC010000901.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073069"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..878
                     /locus_tag="VF276_14740"
     CDS             72..878
                     /locus_tag="VF276_14740"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6608158.1"
                     /translation="MANNDPAAARALVPALDVVGLTKEYGGRRVLDSLDLTLPPQALV
                     GLLGPNGAGKTTLIKLVSGLISPDGGRIRIAGHDLQAQEAQARGALAYVPDVPHFYTE
                     LTVLEHLELLARAHHALEGFPARAEKLLRRFGLWDARDAPPFTLSRGMTQKLALCCGF
                     IRPSQVLLMDEPGGTLDIRSVDTLYALLAEYRQGGGLAVLSSHQWEALQSLCDIFVLM
                     DAGQILAAGHLPFLRDAAELPADAGLREVYLALLEDPSLPDLAGVAEPGA"
     gene            875..>2000
                     /locus_tag="VF276_14745"
     CDS             875..>2000
                     /locus_tag="VF276_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608159.1"
                     /translation="MSALLYLLGHKVARWVPRAGRWVGYDVEKRDLVEQLYGVYIGLF
                     LVGWAVVMLSLATVTISDFVALLKLPVAAFGNLLFFGLAVWLALTPGLAAHSYDLYGF
                     EPADLQFLSNSRLGPGQVAPAWFARSLVRIPAGVFVLVCGILASALQRSGPGDNWWGL
                     GLGWGAGAALYITANALRWAFSLLRYRRVRRFGLLIGYGLTGAALVLLFALPVAWAQI
                     VFWPAAWTSGLIVGGTGPAWPPLAGLLVTAGLAVGGMWAVARTTLLVRALEEGRLGGD
                     MRAASRGGTSAAAEAQLQLHVARRYAAGRLPARIPAAWAARLTGPGGALIYRQWLRAL
                     RLPWGQRLFAVAALLGPACATPIALAALVTPGAELPIVAQV"
BASE COUNT          237 a          667 c          752 g          344 t
ORIGIN      
        1 catgggccgg cggcccgccc ggcggatgcc cttcgataat aacaggatag gcgaaaggaa
       61 cggatagcaa catggccaat aacgatccgg ctgcggctcg tgccctggtg ccggccctcg
      121 acgtggtggg gctgaccaag gaatatggcg ggcggcgtgt gctcgacagc ctggatctca
      181 ccctgccgcc gcaggcgctg gtgggcttgc tggggccgaa cggggcgggc aagacgacgc
      241 taatcaagct ggtgagtggg ctgatttccc cggatggcgg gcgcatccgc atcgccggcc
      301 acgatctcca ggcgcaggag gcccaggcgc gcggcgcgct ggcctacgtg cccgacgtgc
      361 cccacttcta caccgaactg accgtgcttg aacacctgga actgctggcg cgggcgcacc
      421 acgcgctgga gggcttcccg gcgcgcgctg agaagctgtt acgccgtttc gggctgtggg
      481 atgcgcgcga tgcgccgcct ttcaccctct cgcgcggcat gacgcagaaa ctggcccttt
      541 gctgcgggtt catccggccc tcgcaggtgc tgctcatgga cgaaccgggc ggcacgctcg
      601 acatccgcag cgtggacact ctctacgcgc tgctggccga gtaccggcag ggcggcgggc
      661 tggccgtgct cagcagccac caatgggagg cgctccagag cctctgcgac atcttcgtgc
      721 tgatggatgc cgggcagatc ctggccgccg ggcacctgcc cttcctgcgc gatgcggcgg
      781 aactgcccgc cgatgccggg ctgcgcgagg tctatctcgc cctgctggaa gatcccagcc
      841 tgcccgatct ggccggcgtc gcggagccgg gcgcatgagc gcgctgctgt acctgctggg
      901 gcataaggtc gctcgctggg tgccgcgcgc gggccgctgg gtcggctacg acgtggaaaa
      961 gcgcgacctg gtcgagcaac tctacggggt gtatatcggc ctgtttctgg tgggctgggc
     1021 cgtggtgatg ctcagcctgg cgaccgtcac catcagcgat ttcgtcgccc tcctgaaact
     1081 gccggtggcc gctttcggca acctgctgtt ctttggcctg gccgtctggc tggcgctgac
     1141 gcccggcctg gccgcgcaca gctacgatct gtacggcttt gaaccggcag acctccagtt
     1201 cctgagcaac agccgcctgg gtccgggcca ggtggcgccg gcctggttcg cacggtcctt
     1261 ggtgcgcatt ccggcgggcg tgttcgtgct agtgtgcggc atcctggcga gcgcgctgca
     1321 acggagcggc cccggcgaca actggtgggg tctggggctg gggtggggcg cgggcgccgc
     1381 cctgtacatc actgccaacg ccttgcgctg ggcgttcagc ctgctgcgct atcgccgcgt
     1441 gcgccgcttc ggcctgctta tcggctatgg gctgaccggg gcggccctgg tgctgctgtt
     1501 cgcgctgccg gtcgcctggg cgcaaatcgt gttctggccg gcggcctgga ccagcgggct
     1561 gatcgtgggc gggaccgggc cggcctggcc gccgctggcc gggctgctgg tgacggcggg
     1621 cctggccgtg ggcggcatgt gggccgtggc gcgcaccacg ttgctggtgc gcgcgctgga
     1681 ggaagggcgg ctgggaggcg acatgcgcgc cgcgtcgcgg ggtgggactt cggcggcggc
     1741 ggaagcgcag ttgcagctac acgtggcgcg gcgctacgcg gcggggcgct tgcccgcccg
     1801 catcccggca gcgtgggccg cccggctgac cggccccggc ggtgcgctca tctatcgcca
     1861 gtggctgcgg gcgttgcggc tgccctgggg acagcgcctg tttgcggtgg cggctttgct
     1921 gggtccggct tgcgccacgc ccatcgcgct cgccgccctg gtcacgccgg gcgccgagtt
     1981 gccgattgtc gcccaggtcg
//
LOCUS       DASUYC010000902         3692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_843066,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000902 DASUYC010000000
VERSION     DASUYC010000902.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843066"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="VF276_14750"
     CDS             <1..1038
                     /locus_tag="VF276_14750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608160.1"
                     /translation="FQLILGQDDPEAAVGAMAGATLDQPEGGGHLLRKGQRIGPAEVE
                     GLRALAATAPGTRVSLLALDADDVDETEAARMLSAAFAGPGVTLRGPSQGRVRLSAAQ
                     TGLVRVAGARLAAANTLPDISVYTVFDGTPVAAGAPLGEVKITPLAAPRAVIAEAVRA
                     LAGDGAPVLEVRAFTPHRAALVVCERLAPDAAGRMVRGLQNKLAWFGSALDAGDVDHV
                     ANDRAEVAGRLHAAMDRGATLILAAGGSTSDPSDPTLCALADVGATLLRRGVPIHPGS
                     LLWVAQRGEALIMGVPSCGAFGEQTSLDVMLPRLLALGTAGLEGLERLAEGGLLTRGM
                     DHRFPPYGAVE"
     gene            complement(1384..2184)
                     /locus_tag="VF276_14755"
     CDS             complement(1384..2184)
                     /locus_tag="VF276_14755"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6608161.1"
                     /translation="MQFQWLGGPTFLLEWDRFRVLTDPIFGEGPAAFVLNRHPSTGAA
                     QAPIARLAPLPPARLDGLDLLLLSHLHRDHFDAPAAERLDKQVRVAVPRPDAAAVRRW
                     GFASVWGLTWWQYFTLEKDGARLRVTALPARHAADDAVNTDMGLVNGYLIEYRTAQEH
                     YSVYWTGDTVWFDGLTVIREQAGRPDLLVPHMGAVGADGPRGRLTLDATEATHLIDLF
                     QPATVIPVHHHTFAHYVEPIDRLKSALQATPYAGRLVVLREGESWATQ"
     gene            complement(2203..3489)
                     /locus_tag="VF276_14760"
     CDS             complement(2203..3489)
                     /locus_tag="VF276_14760"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX6608162.1"
                     /translation="MTVDNQADTAGQHTPPAGGPAAQNETQAELLATVRLLAGEIGPR
                     PSAGEGERKAAAFVIDRLRHAGYEVELEPFEGLTTYSWTYAPLYGALALAGWLGPRRP
                     ALGALLGLGAFGAFLVESLGFEAVARLPFLPHDRSQNVVARLPLPESSGPPERRVILV
                     AHLDSARSALVFHPRLVRGFRATYVALAAAMATVALGNLARWVQGPRREDPEALDRAT
                     QAAGSALLFPLAVLVQREAAMPVVAGANDNASGVAVVLAAAERLAAALQGALEQKAEL
                     WVVFTGCEESGLGGMQHFLAAHGHELDPATTLFINVDNVGAGMLTLVNQEGTLWPLLA
                     DSELLDAATRMALRRGLHFQTRGYHALPTDAQVPLSRGYRALSVMAFDEHGALPNWHW
                     PTDTPEAVDATTMESALDLLIRTVRRLAAGPGRRIG"
BASE COUNT          519 a         1317 c         1352 g          504 t
ORIGIN      
        1 ttccagttga tcttggggca ggacgatccg gaggcggcgg taggggccat ggcgggggcg
       61 acgctcgacc agcccgaggg gggcggccat ctgctgcgca aggggcagcg gatcggcccc
      121 gcggaggtcg agggcttgcg cgccctggcc gcgacggcac cgggcacgcg cgtctcgctg
      181 ttggcgctcg atgcggacga tgtggacgag accgaagcgg cgcggatgct gagcgcggcg
      241 ttcgccgggc cgggggtgac gctgcgcggg ccgagccagg ggcgcgtgcg cctgtcggcg
      301 gcgcagaccg ggctggtgcg ggtcgcaggc gcgcgcctgg cggcggccaa caccctgccc
      361 gacatcagcg tttacaccgt atttgacggt accccggtgg ccgcgggcgc cccgctcggc
      421 gaggtaaaga tcactccgct ggccgcgcca cgggccgtga tcgcggaagc ggtgcgcgcc
      481 ctggcgggcg acggcgcgcc cgtgctcgaa gtgcgcgcct tcacaccgca ccgcgccgcc
      541 ctggtggtct gcgaacgcct ggccccggac gccgccgggc gcatggtgcg cgggttgcaa
      601 aacaaactcg cctggttcgg ctccgccctg gacgcgggcg atgtggacca cgtggcgaac
      661 gaccgcgcgg aggtagccgg ccggttgcac gcggcgatgg accgcggggc gaccctgatc
      721 ctggccgcgg gcggctcgac cagcgatcca tccgacccga cgctctgcgc gctagccgac
      781 gtgggcgcga ccctgctgcg ccgcggggtg ccgatccacc ccggcagcct gctctgggtg
      841 gcgcagcgcg gcgaggcgct gattatgggc gtgccgtcgt gcggggcctt cggcgagcaa
      901 acctcgctcg acgtgatgct gccgcgcctg ctggcgctgg gcacggccgg gctggagggc
      961 ctggaacggc tggccgaagg cggcctgctc acgcggggca tggaccatcg cttcccgccc
     1021 tacggcgcgg tggagtagcc aaccccgagc actcggatgg gcggcagggc cggtttgaaa
     1081 ccggccccta caccgcaagc cggtgttcca caccagcccg cacgtcgcag ggccggtttg
     1141 aaaccggccc ctacatcgca gaccggcgtt gcacgccaat tcgcacgtcg cagggccggt
     1201 ttgaaaccgg cccctacacc gcagaccggc gttgcacgcc aacccgcacg tcgcagggcc
     1261 ggtttgaaac cggtccctac atcgcagacc ggcgttgcac gccaattcgc acgtcgcagg
     1321 gccggtttga aaccggtccc tacatcgcag gccagtgatc cgtgccaaat atccgcaggg
     1381 cggctattgc gtggcccagg actcgccctc gcgcagcacc accaaccggc cggcgtaggg
     1441 tgtcgcctgc aaggcggact tcagccggtc gatcggttcc acgtagtgcg cgaaggtgtg
     1501 gtggtgcacc gggatgacgg tggccggctg gaacaggtcg atcaggtggg tagcctcggt
     1561 ggcgtcgagc gtcagccgcc cgcgcgggcc gtccgcaccc accgcgccca tatgcggcac
     1621 cagcagatcg ggccgcccgg cctgctcgcg gatcaccgtc aggccgtcga accagacggt
     1681 gtcgcccgtc cagtagaccg agtagtgctc ctgagcggtg cgatactcga tcagatagcc
     1741 gttgaccagg cccatgtcgg tgttcacggc gtcgtccgcg gcgtggcggg cgggcagcgc
     1801 cgtgactcgc agccgggcgc cgtctttttc gagcgtgaaa tattgccacc aggtgaggcc
     1861 ccagacggag gcgaagcccc agcggcgcac cgcggcggcg tcgggccgcg gcacggcgac
     1921 ccgcacctgc ttgtccaggc gctcggcggc gggtgcgtcg aaatggtcgc ggtgcaggtg
     1981 gctgagcagc agcaggtcca ggccgtccag gcgcgcgggc ggtagcggcg ccagccgggc
     2041 aatcggggcc tgggcggccc cggtcgatgg gtggcgattg agcacgaagg cggccggccc
     2101 ctcgccgaag ataggatcgg tcagcacgcg gaaccggtcc cactccagca ggaacgtcgg
     2161 cccgcccagc cattggaatt gcatcggtgt tctccccgcc cgctagccga tgcgccggcc
     2221 cggccccgcc gccaggcggc gcaccgtgcg aatcagcaag tcgagcgcgc tttccattgt
     2281 cgtcgcgtcc acggcctcgg gggtgtcggt gggccagtgc cagttgggca gggcgccgtg
     2341 ctcgtcaaag gccatcacgc tcagggcgcg gtagccgcgc gacagcggca cctgggcgtc
     2401 ggtcggcagg gcatggtagc cgcgggtctg gaagtgcagc ccccggcgca gcgccatgcg
     2461 cgtcgccgcg tcgaggagtt cgctgtcggc cagcaacggc cagagcgtgc cctcctggtt
     2521 gacgagcgtg agcatccccg cgccgacgtt gtccacattg ataaataatg tggtcgccgg
     2581 gtcgagttcg tgcccgtggg ccgccaggaa gtgctgcata ccgcccaggc ccgactcctc
     2641 gcagccggtg aacacgaccc agagttcggc cttctgctcc agcgccccct ggagggcggc
     2701 agccagccgc tcggcggcgg ccaggacgac ggccaccccg ctggcgttgt cgttggcccc
     2761 cgcgacgacc ggcatggcgg cttcgcgctg caccagcacg gccagcggga acaacagggc
     2821 gctgcccgcc gcctgcgtgg cccgatccag cgcttccgga tcctcgcggc gcggcccctg
     2881 gacccagcgg gccaggttgc ccagcgccac ggtcgccata gccgccgcca gcgccacata
     2941 ggtcgcgcga aacccgcgca ccaggcgcgg atggaacacc agcgccgagc gcgcgctgtc
     3001 gagatgggcg accaggatca cgcggcgctc cggcgggccg ctcgattccg gcaacggcag
     3061 gcgggcgacg acgttctggc tgcggtcgtg cggcagaaac ggcaggcggg caacggcctc
     3121 gaagcccagg ctctcgacca ggaaggcgcc gaacgcaccc agccccagca gggcgcccag
     3181 cgcgggccgg cgcggcccca gccagccggc cagggccagc gccccgtaga gcggcgcata
     3241 ggtccagcta taagtggtca gcccctcgaa cggctccagc tccacctcgt atccggcatg
     3301 gcgcaggcgg tcgatgacaa aggccgctgc cttacgctcg ccttcgcccg ccgaggggcg
     3361 cgggccgatc tcgccggcca gcaagcgcac cgtcgccagg agttcggcct gggtctcgtt
     3421 ctgcgcggcg gggccgccgg ccggcggggt gtgctgcccg gcggtatccg cctggttgtc
     3481 cacggtcata acgtgctcct ttaacggtct gtcgctcccg gtgcgcacgc attcacggcg
     3541 ccgtccacgg catttcacgc ccggcgggcc gcattccggt cctgcccccg gccatcgggc
     3601 ggggcgccgg ccctggagcc tgcctgcggt atcccggcgt cgtatggagg atgacgaatc
     3661 taacggatct cccccgcggt tcgttcgctg cc
//
LOCUS       DASUYC010000903         3595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_929010,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000903 DASUYC010000000
VERSION     DASUYC010000903.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929010"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            291..1655
                     /gene="mgtE"
                     /locus_tag="VF276_14765"
     CDS             291..1655
                     /gene="mgtE"
                     /locus_tag="VF276_14765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX6608163.1"
                     /translation="MVTYTDREQGEAQIQAVLAGGDPAALRAVVTMYHPADIADLLDR
                     LDAPQRLRVFQALDPAAAAEVLDEAGAGVARSLLRALPPASAGALLDRLPPDDAADVL
                     GEDGPGHQQALLATMTPADAAHVRTLLQYPPKTAGRLMLDRFVPLRPEMTASAALAYV
                     RQVADARAPLDNLYVLDSAGRVAGVVALREVVLAPPTRLLSEFMTTRIIQVTPETDQE
                     EVARLIAQYDLTSLPVVTADARLLGIVTVDDAIDVLLEESNEDVLRFGGVEGPANDQP
                     YFTVSIPQAVRRRVFALLLLFVAESFTGSVLRLFEDELSQVVALSFFIPLLIGTGGNT
                     GAQTVSTLIRGLAFGEIRLRDTWRVLARELGSGFLLGLLLGSVGFARALLWNSSVPLA
                     LVVGLTLVVICTWANTVGSLIPLLAHRVGVDPAVISAPLITTLVDATGLVIYLLIAKA
                     LLGL"
     gene            1888..2643
                     /gene="lepB"
                     /locus_tag="VF276_14770"
     CDS             1888..2643
                     /gene="lepB"
                     /locus_tag="VF276_14770"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX6608164.1"
                     /translation="MQLDEPQARVATVPPPASVPVSEQPHYLLPAGGADISVREVSYG
                     SKSIAHELIEAVVLSLLIFLLVQSVVQNRKVVGASMEPSLRNEEHLLIDRASYFRYDT
                     NFLPDLLGAQAPANYQFLLGGPQRGDIVVFRPPVQGESEDYIKRVIAVPGEAVQVKAY
                     DGVYVNGHKLDEPYIKDTPDYNWPETGGAEVVPAGHLFVLGDNRRNSSDSHAWGFVDM
                     TSIVGKAWISYWPREVLGFLPHPTYAGVDTPAR"
     gene            2811..>3595
                     /locus_tag="VF276_14775"
     CDS             2811..>3595
                     /locus_tag="VF276_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEX6608165.1"
                     /translation="MRLDLPLDQVLIGDCRDVLATLPDKSVDLIFADPPYNLQLQQEL
                     WRPNMTRVDGVDAAWDQFADFAAYDRFTEAWLEACRRVLKDTGALWVIGSYHNIYRVG
                     KILMDLGYWILNDISWQKVNAMPQFRGVRFTNAHETLLWAKKSRTQKRYTFNYHAMKN
                     LNDEKQMRSDWEIPLCTGAERLKVDGHKAHPTQKPEALLYRVILATSNPGDVVLDPFF
                     GTGTTGAVARTLHRHWIGIERDPGYADLARARIASVQPALLSEA"
BASE COUNT          587 a         1138 c         1203 g          667 t
ORIGIN      
        1 tcctggcgat cgaccaggtg gtgggcaagg cctggctatc ctactggccc agcgacctgc
       61 tgggtttcct gccccatccc acttacgcca acgtagtgca gccgccggtc gttccctaac
      121 agcggtaaac catacgctag acatcgggga gcctttggct ccccatattt tatgtcccgc
      181 gctcggcagc gcggaattgc tatacccgga gaggggtgag ggtgcatcaa gctgcaccgg
      241 gccgtttcat tttgcgctgg agatttgatc ggtggcaagg agactcggct atggtgacgt
      301 atactgatcg ggaacaaggc gaggcgcaga tccaggccgt cctggcgggg ggcgaccccg
      361 ccgccctgcg ggccgtggtg acaatgtacc acccggcgga tattgccgat ctccttgatc
      421 ggctggatgc gccgcaacgc ctgcgtgtct tccaggcgct ggacccggcg gcggcggcgg
      481 aagtgctgga cgaagccggc gcgggggtgg cccgatcgtt gctgcgcgcg ctgccgccgg
      541 ccagcgcggg ggcattgttg gaccggttgc ccccggacga tgccgctgac gtgctggggg
      601 aggatgggcc ggggcaccag caggccctgc tggcgacaat gacccccgct gatgccgccc
      661 atgtgcggac gctgcttcag tatccaccca aaaccgccgg gcggttgatg ctggaccgct
      721 tcgtaccctt gcgcccggag atgacggcga gcgccgccct ggcctatgtg cgtcaggtgg
      781 cggatgcgcg cgcccccctt gacaacctct atgtgctgga tagcgccggg cgggtggccg
      841 gcgtcgtcgc cctgcgcgag gttgtcctgg cgcccccaac gcgcctcctc agcgagttca
      901 tgacgactcg gattatccag gtaacgccgg agacggacca ggaagaggtc gcccgcttga
      961 tcgcccagta cgacctcacg agcctgccgg tggtcactgc cgatgcgcgg ttgctgggga
     1021 tcgttacggt ggatgatgcc atcgacgtgt tgctggaaga atcgaacgag gatgtgttgc
     1081 gcttcggcgg tgtagagggg ccggccaacg atcaaccgta tttcacggtc tcgatcccgc
     1141 aggcggtgcg gcgccgggtg tttgcgttgt tgctgctttt cgtggccgag agctttaccg
     1201 gcagtgtgct gcgtctgttt gaggatgaat tgtcgcaagt cgtggccctg tccttcttca
     1261 tcccgctgct gatcggcacc ggcggcaaca ccggcgcgca gacggtctcc accttgatcc
     1321 gggggctggc gttcggcgaa atccgcctgc gcgatacatg gcgggtactg gcccgtgagt
     1381 tgggtagcgg cttcctgctg ggcctgctgc tggggtcggt cggctttgcg cgggcgcttc
     1441 tctggaacag ttccgtgccg ctggcgttgg tggtggggtt gacgctggtg gtgatctgca
     1501 cctgggccaa cacggtcggc tccctgatcc cgctcctggc ccaccgggtg ggagtcgatc
     1561 ccgccgtcat ttccgcgccg ttgattacga cgctggttga tgccacgggg ctggttatct
     1621 acctcttaat cgccaaggcg ctgctcggat tgtagcgggg gcggcgcggc caggatctgt
     1681 gcgcgacctt gccgtacaga ggaccggctg gcatgccttc tgcttttgtc cggtgagacc
     1741 tgcgggccgg gtggctcgcg gtcgtagtga cacctaccga tgatccggcg tttggccgac
     1801 ccggtacgtt tccggtataa ttgccgtact atgcggcgcg caggtgcaag cggcgtgccg
     1861 atccgttctg ggaggactcc gatacccgtg caactggacg aacctcaagc gcgcgtggcg
     1921 accgtgccgc ccccggcgag cgtgccggta agcgaacagc cccactatct gttgcctgcc
     1981 ggcggcgccg acatcagcgt gcgcgaagtc agttatggca gcaaatcgat cgctcacgag
     2041 ttgatcgagg cagtggtgct gagtctgctg atcttcctgc tggtgcagag cgtggtgcag
     2101 aaccgcaaag tcgtcggcgc cagcatggag ccgagcctgc gcaacgagga gcatctgctg
     2161 atcgaccgcg cgtcgtattt ccgctacgat acgaactttc tgcccgacct gctcggtgcg
     2221 caggcgcccg ctaattacca gttcctgctg ggcggaccgc agcgcggcga tatcgtggtc
     2281 ttccggcccc cggtgcaggg cgaatccgag gactatatca agcgcgtgat cgccgtgccg
     2341 ggcgaggcgg tgcaggtcaa ggcctacgac ggcgtctacg tgaacggcca caaactcgac
     2401 gagccgtata tcaaggacac gcccgattac aactggccgg agacgggcgg ggcagaggtg
     2461 gtgccggcgg ggcacctgtt cgtgctgggc gataaccgcc gcaacagcag cgactcccac
     2521 gcctggggct tcgtcgatat gaccagcatc gtcggcaaag cctggatttc gtactggccg
     2581 cgcgaggtac tgggcttcct gccgcacccc acctacgccg gtgtggatac gccggctcgg
     2641 taatcacccc gaacgcagca catggcgggc ggcggcgcaa agctgccgcc tttttgcatc
     2701 cccgcagcta tcgacttgcg actcccccct gtgctataat caggatgaca taactatgcg
     2761 gatcgggtga caggcgagcg tgcgctcgtc cggcgatccg gtgggagagg atgcgcttgg
     2821 acctaccact cgaccaggta ctgatcggtg attgccgcga tgtactcgcc acgctgcccg
     2881 acaagagcgt ggacctgatt ttcgccgacc cgccgtacaa tctccagttg cagcaggaac
     2941 tctggcgccc caacatgacc cgcgtggacg gcgtggacgc cgcctgggac cagttcgcgg
     3001 acttcgccgc ctacgaccgc ttcaccgaag cctggctgga ggcctgccgg cgcgtgctca
     3061 aagacaccgg cgccctctgg gtcatcggca gctaccacaa catctatcgc gtgggcaaga
     3121 tcctgatgga cctggggtac tggatattaa atgacatctc gtggcagaaa gtgaacgcca
     3181 tgccccaatt tcgcggcgtg cgcttcacca acgcgcatga gacgctgctg tgggccaaga
     3241 agtcgcgcac gcagaagcgc tatacgttca actaccacgc gatgaagaac ctgaacgacg
     3301 agaagcagat gcgtagcgac tgggagatcc cgctctgcac cggggccgag cgcctgaagg
     3361 tggacgggca caaggcccac cccacgcaaa agcccgaggc gctgctctac cgcgtgatcc
     3421 tggcgaccag caaccccggc gacgtggtgc tcgacccgtt cttcggcacg ggcaccaccg
     3481 gggccgtcgc gcgcacgctg caccggcact ggatcggcat cgagcgcgat ccgggctacg
     3541 ccgacctggc ccgtgcgcgc atcgcgtcgg tgcagcccgc cttgctgtcg gaggc
//
LOCUS       DASUYC010000904         2149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1073931, whole genome shotgun sequence.
ACCESSION   DASUYC010000904 DASUYC010000000
VERSION     DASUYC010000904.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073931"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VF276_14780"
     CDS             complement(<1..327)
                     /locus_tag="VF276_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608166.1"
                     /translation="MNDRYDEADRDRRLLAMIDELQAGTSPAPPTGEPLHDFAALLHG
                     AAPEPDPGFQQKLDARLTAAVRDRTARAPARPGAVQRGPTLPGNAGNGGWRRLFRPVA
                     GVTRGLA"
     gene            complement(324..893)
                     /locus_tag="VF276_14785"
     CDS             complement(324..893)
                     /locus_tag="VF276_14785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6608167.1"
                     /translation="MPLDDEQHLLVLARQDPQAFRRIYQHYVPRLYAYVRYRVERKQD
                     AEDLVAETFLRVVDGLDRFEWRHEGSFAAWLFRIAHNAISNFHRDHAHLDYTRAPEEL
                     PEPVATGPLPDDIVLRKELFADLHRLIATLPPRRREVITLKFFGGLQNREIAAVLGLD
                     ERTVAAHLCRALEDLHARYTRVEDKVSNP"
     gene            1352..2065
                     /locus_tag="VF276_14790"
     CDS             1352..2065
                     /locus_tag="VF276_14790"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX6608168.1"
                     /translation="MDNNRHSTTPSDTPFGCVWDLDGVLVDSGVAHRAAWTALAEELG
                     FSWSDELHTRTFGMRNPDIIRIAWGVEGPPDKVNQWGEHKEELFRDQARGLHALPGAA
                     ELVRDLHAHGWRQAIGSSAPAANVALLLEVLKLTRYFDSVVSGDDITRGKPDPEVFRT
                     GFRRLEVPPARGVVIEDAVAGVQAGVAAGAFTIGVTNTRGRADLEAAGAHLVVDSLAE
                     LDAARLEAAVRGFRAGRSA"
BASE COUNT          308 a          714 c          772 g          355 t
ORIGIN      
        1 ggcgagtccg cgcgtcacgc cggccaccgg gcggaacagc cgccgccacc cgccgttgcc
       61 ggcgttgccg ggcagggtgg ggccgcgctg aacggcgccg gggcgggcag gggcgcgggc
      121 ggttcggtcg cggacggcgg cggtgagccg ggcatcgagc ttttgctgga agccggggtc
      181 gggttcgggc gccgcgccgt gcagcagggc ggcaaagtcg tgcagcggtt cgcccgtggg
      241 cggcgcggga ctcgtgcccg cctgtagctc gtcgatcatt gccagcaagc gccggtcgcg
      301 atccgcttcg tcgtatcggt cgttcatggg ttgcttacct tgtcttccac gcgcgtatag
      361 cgggcgtgca ggtcttcgag tgcccggcag agatgcgcag ccaccgtccg ctcgtccagg
      421 cccagcacgg cggcgatctc gcggttctgt agcccgccga agaatttcag ggtgatcact
      481 tcgcgccggc gcggcggcag cgtcgcgatc agccggtgga gatcggcgaa cagttccttg
      541 cgcagcacga tgtcgtcggg cagcgggccg gtcgccacgg gttccggcaa ctcctccggc
      601 gcgcgggtgt agtcgagatg cgcgtggtcg cgatggaagt tgctgatcgc gttatgggcg
      661 atacggaaga gccacgccgc aaagctgccc tcgtggcgcc actcgaagcg gtcgaggcca
      721 tccacgaccc gcaggaacgt ttcggccacc aggtcttcgg cgtcctgctt gcgctcgacg
      781 cggtagcgga cataggcgta gagccggggc acgtagtgct ggtagatccg gcgaaacgcc
      841 tgggggtcct ggcgggccag cacgagcaag tgttgttcgt cgtctaaagg cattccggcc
      901 ggttcctcat ctcgcggtct gtctggttgg tctgcttgtt gtcctgttta ttgtacgcgc
      961 acatccgtaa taacaccgaa tcccgaaaaa tactgcatcc cggcccggtc ccacaagctt
     1021 ccgttgcccc gcgcaggatc gcggcattgc cttgcgcggc gcccagaggc gggcgccggg
     1081 tgcgcgtcat gcttttgcac gcgcaaggcg gcatcggggc gcgcgaccaa cctaaccccc
     1141 cgtccccctt cccgccgtgg gaagggggaa ccgctttcac catcgaacgt gactcctcgc
     1201 ggcggggagg ggcgggggga gaggttgccc cgcccacccg cgggcgcccg cgaaggggac
     1261 gaaatcgggc cattgtgcta tactggcacg gggtctcgtg ccaggggccg ccacaagtgc
     1321 aggtcaggta aggagtgcgt gttacgcaat tatggacaac aatcgacatt cgacaacacc
     1381 atcggacacg cctttcggtt gtgtctggga cctggatggc gtactggttg acagcggggt
     1441 cgcccaccgg gcggcctgga ccgcgctggc cgaggaactg gggttctcct ggtcggacga
     1501 gctgcatacg cgcaccttcg gcatgcgcaa ccccgacatc atccgcatcg cctggggcgt
     1561 cgagggtcca cccgacaaag tcaaccagtg gggcgagcac aaggaagagc tcttccgcga
     1621 ccaggcgcgc ggcctgcacg ccttgcccgg cgccgccgag ttggtgcgcg acttgcacgc
     1681 ccacggctgg cgccaggcca tcgggtcgtc cgcgcccgcg gccaatgtgg cgctgttgct
     1741 cgaagtgctg aagctgaccc gctatttcga ctccgtggtc tccggcgacg acatcacgcg
     1801 cggcaagccc gacccggagg tcttccgcac cgggttccgg cgcctggagg tgccgcccgc
     1861 gcgcggggtg gtcatcgaag acgccgtggc cggtgtgcag gccggggtgg cggcgggcgc
     1921 cttcaccatc ggcgtcacca acacccgtgg ccgggccgac ctggaagcgg cgggcgccca
     1981 tcttgtcgtg gacagcctgg ccgagctcga tgccgcgcgc ctggaggcgg ctgtgcgcgg
     2041 cttccgggcc gggcgcagcg cctagcgccg gttggccgtg gccgcctacg tctacctcct
     2101 gctctgcgcc gacggcacct actataccgg ctggaccacc gacgtggcg
//
LOCUS       DASUYC010000905         5573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_636584,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000905 DASUYC010000000
VERSION     DASUYC010000905.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636584"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="VF276_14795"
     CDS             <1..687
                     /locus_tag="VF276_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX6608169.1"
                     /translation="EGIIRTVRSQIEETVYRLFSWTEGDFRFEPDVKPPRESVTIPLS
                     IENVIMEGVRRIDEWSRIRDRIPTLDVIVRFADQPTERAKGVNLTPDEWRVFARINGA
                     DNIAAIARLTQLSEFDVCRIIYGFIQAGLVEVARPQVMVAPEVQARARALVEDTLGDG
                     TMPVDAATLANLPPLSAPKGASLEATPAHPAPVPVAVGPQQAQAPAVQNTVNVKRGVI
                     FRIIERIRRI"
     gene            733..1266
                     /locus_tag="VF276_14800"
     CDS             733..1266
                     /locus_tag="VF276_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HEX6608170.1"
                     /translation="MHTVKMVITGAVNSGKSQFIRTISEIEVVSTERKATDEETRRLK
                     KETTVAMDFGRIAVAEDIVLHLFGTPGQKRFDFMWEILSEGMLGLVVLVDSTRPDTFR
                     ETLRIIDFFNSYRPMPFVVAANKQDEDNAWKPDEVRLALRLPREVKVLPLVAKDREQV
                     KNVLLELLYTILAVAEE"
     gene            1339..1707
                     /locus_tag="VF276_14805"
     CDS             1339..1707
                     /locus_tag="VF276_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEX6608171.1"
                     /translation="MATRTEEMVARLRNLAASTADIEASAVVSVDGLIMASALPRDVE
                     EDRVSAMSAAMLSLGERIAIELGRGTLDQVFIRGEQGYVLLMSVGTEAVLTALARKEA
                     KLGLVLLDMRRTAAELSRLI"
     gene            1904..3040
                     /gene="mnmA"
                     /locus_tag="VF276_14810"
     CDS             1904..3040
                     /gene="mnmA"
                     /locus_tag="VF276_14810"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007934012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX6608172.1"
                     /translation="MAKKVIVAMSGGVDSSLTAALLQDRGYEVIGVHMRLHALHAETG
                     TPEGRGRLNHGCCSLEDVDDARMVAYELGIPFYVLNFEPEFRKHIINNFVQEYSRGRT
                     PYPCLNCNRYVKFDALLERAEALGADYLATGHYARIVQDAAGQYHLRRAADRAKDQAY
                     VLYHLNQEALSRLLLPLGDFDKPTVRRLAAAKNLVVADKPDSQEICFIPDNNYRNFLE
                     KMQPGMAQPGPMVNTRGEVVGRHTGLPHYTVGQRKGLGALGAEPYFVLRIDPGSNTLL
                     VGTAAELASYSLTADDVHFIAGTRPSAPFPALVKIRYRAPETPALVTPLDDGRVRVDF
                     DTPQRAVTPGQAVVFYTDDEVLGGGTIENPAVAAPGAPAATAAA"
     gene            3037..3291
                     /locus_tag="VF276_14815"
     CDS             3037..3291
                     /locus_tag="VF276_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608173.1"
                     /translation="MTLSPTVLVFLGFGTAYALLFHLWRGRNIQQLILFWLVSILGFG
                     LGFGLAGLLNVHLLLLGGVPVVEASIGALLLLIAAQRLVA"
     gene            3362..3757
                     /locus_tag="VF276_14820"
     CDS             3362..3757
                     /locus_tag="VF276_14820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608174.1"
                     /translation="MRNVFIGIAAVLVVIAAIAPLLIAFFIPDWIPGMQAVAVAYVSA
                     FACVGAILFIVLVGLLALIAFQLKNNVVPLLEKATDTMHTVQGTTTFVSESVVQPIIK
                     TAGAVAGARAMVQTLMRRQGGTDTTGNGR"
     gene            3754..3999
                     /locus_tag="VF276_14825"
     CDS             3754..3999
                     /locus_tag="VF276_14825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608175.1"
                     /translation="MSTPRKSADASFAMGLVLGLLVGAGVVIVLSPQFRTQANRVARE
                     LGLSTGADGRVMAEHGRDTLLKEVAPPPNPLSTTAEA"
     gene            complement(4072..4869)
                     /locus_tag="VF276_14830"
     CDS             complement(4072..4869)
                     /locus_tag="VF276_14830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608176.1"
                     /translation="MDDSVRAEYQRRLNAGDRGGALAYLRMLRTQTDDPSARDAYTRA
                     IDSIENGGDGSDMAAASVSTADSAPLYGGSAGPFGDNPDEWAATHQPRHDTGVAAGAS
                     GTTAQAPAEAPAQPPVWAGPSTPPAAAPAPAPAPAPEHHGPSDPISEAIGGLQRGIHD
                     RLTGQHHEEPAQAQAPAPAPAPAPEHHGPSDPISEAIGCLQRGIHDRIFGHDDKAPHT
                     AQAQTESATFGPSADEQAGTRGKIADEDAGTRGKIADEDRSGGGDIV"
     gene            complement(5086..>5573)
                     /locus_tag="VF276_14835"
     CDS             complement(5086..>5573)
                     /locus_tag="VF276_14835"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX6608177.1"
                     /translation="GTFSIQRRTLDLVALLHDEIEAARVTSSRHAISLDAPAELPVWG
                     DSLRLAEVVQNLLSNAIRYSPQGGPIQVLAGVVEGAAQISVCDAGMGIAPEDLPRVFD
                     RAYRGRGEQIGGGHGLGLYISREIVRLHGGRIWAESVAGQGACFYVVLPLGDAPASLA
                     P"
BASE COUNT          913 a         1854 c         1904 g          902 t
ORIGIN      
        1 gaaggcatta tccgcacggt gcgcagccag atcgaagaaa cggtctaccg gctcttctcc
       61 tggacggagg gcgatttccg gttcgagccg gatgtcaagc cgccccgcga gagcgtgacc
      121 attccgctca gcatcgagaa cgtgatcatg gagggcgtgc ggcgcatcga cgagtggtcg
      181 cggatacggg accgcatccc gacgctcgac gtgatcgtgc gcttcgccga ccagccgacg
      241 gagcgggcca agggcgtgaa cctgacgccc gacgagtggc gagtgttcgc ccgtatcaac
      301 ggggccgaca acatcgccgc catcgcccgg ctgacccaac tgagcgagtt cgacgtgtgc
      361 cgcattatct atggctttat ccaggccggc ctggtcgaag tggcgcgacc gcaggtcatg
      421 gtcgcgcccg aggtccaggc gcgcgcgcgg gcgctggtcg aggacacact aggcgacggc
      481 acgatgccgg tcgacgcggc gacgctggcg aacctgcccc cgctgtctgc ccctaaaggg
      541 gcatctttag aggccacgcc cgcgcacccg gcccccgtgc cggtcgccgt ggggccgcag
      601 caagcgcagg cgcccgccgt ccagaacacg gttaacgtca agcgaggggt catcttccgg
      661 atcatcgaac ggatccggcg catctaactc agaagcgccc ggcgcgactt tacaacagcg
      721 gaggatagac cggtgcatac ggtcaaaatg gtcatcaccg gggcggtgaa ctcgggcaag
      781 agccagttca tccgcacgat cagcgaaatc gaagtggtat caacggaacg gaaggcaacc
      841 gacgaggaga cacgccgttt aaaaaaagag acgaccgtgg cgatggactt cgggcggatc
      901 gccgtcgccg aggacatcgt gctgcacctg ttcgggacgc cggggcaaaa gcgcttcgac
      961 ttcatgtggg agattctctc ggaagggatg ctgggcctgg ttgtgctggt ggacagcacc
     1021 cgccccgata ccttccgcga aacgctgcgc atcatcgact tcttcaactc ctaccggccg
     1081 atgcccttcg tggtcgcggc gaacaagcaa gacgaggata acgcctggaa gccggacgaa
     1141 gtgcgcctgg ctttacgctt accgcgcgag gtaaaggtgc tcccgttagt ggcgaaagat
     1201 cgggagcagg tcaagaacgt gctgctggaa ctgctctaca cgatcctggc ggtcgccgaa
     1261 gagtagcccc ggcaccggac ggcccggcgc gcgggtcgag acacagacat ctcatacctt
     1321 cccaaatgga ggaggaacat ggcgacacgg acagaagaaa tggtggcgcg gctgcgcaat
     1381 ttggcggcca gcacggcgga catcgaagcg tcggcagtgg tgagcgtcga tgggctgatc
     1441 atggcctccg cgctcccgcg cgacgtggaa gaagaccgcg tctccgccat gagcgccgcc
     1501 atgctctcgc tgggcgagcg cattgccatc gaactgggcc gcggcaccct ggatcaggtg
     1561 tttatccgcg gcgagcaagg ctatgtgctg ctcatgtcgg tcgggacgga agcggtgctg
     1621 acggcgctgg cccgcaagga ggccaagctg ggcctggtgc tgctcgacat gcgccgcacc
     1681 gccgccgagt tgagccggct catctaggaa ccgcgttccg ggacgcgggc acggttcctg
     1741 ccacgcgaac agaggatagg aaccgtgcag acggccggcc gccaccggca cgggcgctgc
     1801 gcagccgggc ctaccgtgcc cggcttcgtc gtgtctgcgc tcggtcagat tagtattagc
     1861 aacgtaatgg cgcacagggt gcgccctggt tagagaggat cgcatggcga agaaggtcat
     1921 tgtcgccatg agcggcgggg tcgatagctc gctcaccgcc gccctgttgc aggaccgcgg
     1981 ttatgaggtg atcggcgtgc atatgcgcct ccacgcgctg cacgccgaga cgggcacgcc
     2041 cgaggggcgg ggccggctca accatggctg ctgctcgctc gaagatgtcg atgatgcgcg
     2101 catggtggcc tatgagctag gcatcccctt ctatgtgctg aacttcgagc ccgagttccg
     2161 caagcatatc atcaacaact tcgtccagga gtacagccgc gggcgcacac cctatccatg
     2221 cctgaattgt aaccgttatg tcaagttcga cgccctgctg gagcgggccg aggctctggg
     2281 cgccgactac ctggcgaccg gccactacgc ccgcatcgtg caggatgccg cggggcaata
     2341 ccacctgcgc cgagcggcag accgcgccaa ggaccaggcc tatgtgctct accacctgaa
     2401 ccaggaagcg ctctcgcgcc tgctgctgcc gctgggcgac ttcgacaagc cgacggtgcg
     2461 ccgcctggcc gccgcgaaga acctggtggt ggccgacaag cccgacagcc aggagatctg
     2521 tttcatccct gacaacaact accgcaactt cctggagaag atgcagccgg gcatggccca
     2581 gccggggccg atggtcaaca cgcgcggcga ggtcgtgggc cggcacacgg gtctacccca
     2641 ctacacggtg ggccagcgca aggggctggg cgcgctcggc gccgagccgt acttcgtgct
     2701 gcgcatcgac cccggcagca acacgctgct ggtgggcacc gccgccgaac tcgcgtcgta
     2761 cagcctcacc gccgacgacg tgcatttcat cgccgggacg cgcccgagcg cgccgttccc
     2821 cgccctggtc aagatccgct atcgcgcgcc ggagacgccg gccctcgtga ccccgctgga
     2881 tgacgggcgg gtgcgcgtcg atttcgacac cccgcagcgg gccgtgaccc ccggccaggc
     2941 agtggtgttc tacaccgacg acgaggtgct gggcgggggc accatcgaga acccggcggt
     3001 ggcggcgcca ggcgcccccg ccgcgaccgc cgccgcatga cgctctcgcc taccgtgcta
     3061 gtgttcctgg gcttcggcac ggcctatgcg ttgttgttcc acctctggcg cgggcgtaac
     3121 atacaacagt taattctgtt ttggttagtg agcatcctgg gatttggcct ggggttcggt
     3181 ctggccggcc tgctgaacgt ccacctgctg ttgctggggg gcgttccggt ggtcgaggcc
     3241 tccatcggcg cgctgctgtt gttgatcgcg gcgcaacgcc tcgtggcctg accgcggcct
     3301 cgatcctggg gtgggcacct gagatgcccc actcaattcc gcaccgagga gaccgcagta
     3361 catgaggaat gtctttatcg gcatcgccgc tgtcctggtt gtgattgccg cgattgcccc
     3421 cttgctgatc gctttcttca tcccggattg gattccgggg atgcaagcgg tcgcggtggc
     3481 ctatgtctcg gcgttcgcct gtgtcggcgc catcctgttc atcgtgctgg tcggcttgct
     3541 ggccctgatc gccttccagc tcaagaacaa cgtcgtgccg ctgctggaga aggccacgga
     3601 caccatgcac accgtccagg gcacgaccac cttcgtcagc gaaagcgtgg tccagccgat
     3661 cattaagacc gcgggcgcgg tcgccggggc gcgggccatg gtgcagacgc tgatgcggcg
     3721 ccagggcggc accgacacca ccgggaacgg gcgatgagca cgccgcgcaa gtccgccgat
     3781 gccagcttcg ccatggggtt ggtgctgggg ttactggtcg gcgcgggggt ggtcatcgtc
     3841 ctctcgccgc agttccgcac gcaggccaac cgggtggccc gcgagttagg gctgagcacg
     3901 ggcgccgatg ggcgcgtcat ggcggagcac gggcgcgaca ccctgctcaa ggaagtcgcg
     3961 ccgccgccca acccgctcag caccaccgcc gaggcctgag accacacggg acgcaaaccg
     4021 ccggggtagc atcagccgcc ccggcgtttc tgtgtgcgct atcgcagctg gctagacgat
     4081 gtcgccgccc ccgctgcgat cctcgtcggc gatcttgccg cgcgtacctg catcctcgtc
     4141 ggcgatcttg ccgcgggtgc cggcttgctc gtcggcactc ggaccaaacg tagccgactc
     4201 ggtttgcgcc tgcgccgtgt gtggcgcctt gtcgtcgtgg ccgaaaatgc ggtcatggat
     4261 gccgcgttgc aggcagccga tggcctcgct gatcgggtcg ctcggcccat ggtgctccgg
     4321 agcgggcgcc ggcgccggcg cgggggcctg cgcctgggcc ggctcctcat ggtgctggcc
     4381 ggtcaaccga tcatggatgc cgcgttgcaa gccgccgatg gcctcactga tgggatcgct
     4441 cggcccatga tgctccggcg cgggcgccgg agcgggggcc ggcgcggcgg caggcggcgt
     4501 cgaaggccca gcccagacgg gtggctgggc gggcgcctcg gccggggcct gggcggtcgt
     4561 tccgctggcg ccggccgcga cgccggtgtc gtggcgcggc tgatgggtgg ccgcccattc
     4621 gtcggggttg tcgccgaacg gcccggcgga cccgccgtac aacggtgcac tatcggcggt
     4681 gctgacggac gccgccgcca tatccgagcc gtcgccgccg ttctcaatcg aatcgatggc
     4741 ccgcgtatag gcatcgcgcg ccgacgggtc gtcggtttgc gtccgcagca tccgcagata
     4801 cgctaacgcc ccgccgcggt ccccggcgtt gagccggcgc tgatattcag cgcgaacact
     4861 gtcgtccata tgttactcct ccacttgcct ggcgaaaatc agccggccgc catagctgag
     4921 tccggcctga tctaccgagc aagaaaagag ccaagcgttg gacggggcgg gcactgcggc
     4981 ttggggactg tagccgacca gtgaatgtgt ttatgtaggg gcggacccgc gggtccgccc
     5041 tgctgccaac gctgtagggg ccggttgcaa accggcccgc ggccattacg gcgccaggct
     5101 cgccggcgcg tcgcccagcg gcagcaccac gtagaagcag gcaccctgcc cggcgacgga
     5161 ctcggcccag atgcgcccgc cgtgcaggcg cacgatctcg cggctgatgt agaggccgag
     5221 gccatgcccg ccgccgatct gctcgccccg cccgcggtag gcgcgatcga acacgcgcgg
     5281 caggtcttcg ggcgcaatgc ccatgccggc gtcgcagacg ctgatttgcg cggcgccttc
     5341 gacgaccccc gccagcacct ggatcggccc gccttgcggc gagtagcgga tggcattcga
     5401 cagcaggttc tgcaccacct cggccaggcg caaggagtcg ccccagacgg gcagttccgc
     5461 gggcgcgtcc aggctgatcg cgtgccggct ggaggtgacg cgcgccgcct cgatctcgtc
     5521 gtgcagcagc gccaccaaat ccagggtccg gcgctggatg ctgaaggtcc ccg
//
LOCUS       DASUYC010000906         3228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_967119,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000906 DASUYC010000000
VERSION     DASUYC010000906.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967119"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="VF276_14840"
     CDS             <1..1050
                     /locus_tag="VF276_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608178.1"
                     /translation="DVVQRALGGQSEVDAHLVDARDQHHRVDPGFGGQQRGGPVLVDD
                     RVHGPPLAVLANHGDAPAARGDHDRADWCRAQIARLQTAIHESAWDGAWYRRAYFDDG
                     TPLGSHENEECRIDSIAQSWSVLSGIGDPERAQQAMASLEEHLIRPAESLVLLLTPPF
                     DKSDLDPGYIKGYVPGVRENGGQYTHAAIWVGFAFAELRDGVRAHALFNMLNPINHAL
                     DPDDRDRYKVEPYVIAADVYAHPQHVGRGGWTWYTGSAAWMYRLGVEAILGLRRVDGG
                     LCVDPAIPPHWPGFEVCYRHGAATYTIQVDNTAGGGHGVVAVTLDGAPCPDGRIPLDP
                     APGTHAVEVRLGPGE"
     gene            1379..1804
                     /locus_tag="VF276_14845"
     CDS             1379..1804
                     /locus_tag="VF276_14845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608179.1"
                     /translation="MHYNSVDHFLTAHESQIIDMMASAQQATGGHYAGLSEAARRADA
                     QAAAQRMMAAVRQVQLDGQTVQATARQRDSAGIPLDDLTRLVDQMETRFIAFVQAELG
                     AQPELAAEILRRLRNANARFRSNVMAVKLDKTIRQLQRR"
     gene            complement(1836..2999)
                     /locus_tag="VF276_14850"
     CDS             complement(1836..2999)
                     /locus_tag="VF276_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006990035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033926 - glycopeptide
                     alpha-N-acetylgalactosaminidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 100 family protein"
                     /protein_id="HEX6608180.1"
                     /translation="MAADDRVIEEARRAALEVLHYCARGPRGELPRTAGWGYPEPYTR
                     DWMIAALGILTTGDPLLLDTIRRMLSALAAIQSPHGQIPGLADAPADRGSSDTTPLFL
                     IGLALVRRVTGETAFLEAAAARARRWMAYQSDDDGYLVAQQPTSDWRDEQWVLGHGLY
                     VNTLVYAYLRLYGEAAQAAGLAAQINRPIQLLAHDNPHGAEGLLLPDAPYYALWAYKV
                     WGSTRFDLLGNSLAILTGLASPDRARAMVAWIEAECAELRARGDLALDLPPCLFPYIR
                     PADPDWFPRYAEFNQPGEYHNGGIWPFVCGFYVAALVAAGEQALAARKLAALAALVRP
                     ARNHAVAFGFNEWIRAQDGTPRGEDWQVWSAALYLYALAAVEQGQTPFFDRVR"
     gene            complement(3039..>3228)
                     /locus_tag="VF276_14855"
     CDS             complement(3039..>3228)
                     /locus_tag="VF276_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014054541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEX6608181.1"
                     /translation="DADTKQILGCAVLGLEGGEIMAQLQIAMMGNLPYTVLRDATFAH
                     PTLAESLNNLFTNMDAEG"
BASE COUNT          525 a         1129 c         1114 g          460 t
ORIGIN      
        1 gacgtcgtgc aacgcgcgct gggtgggcag tcggaagtcg acgctcacct cgtcgacgcc
       61 cgtgaccagc accatcgtgt cgaccccggc ttcggcggcc agcagcgcgg aggaccggtc
      121 cttgtcgacg accgcgtcca cggccctcca ctggccgttc tcgcgaacca cggggatgcc
      181 cccgccgccc gcggcgatca cgaccgcgcc gactggtgcc gggcgcagat cgcccggctg
      241 caaaccgcta tccacgaatc ggcctgggac ggggcgtggt atcgccgcgc ctatttcgac
      301 gacggcacgc cgctcggctc gcacgagaac gaggagtgcc gtatcgactc catcgcgcaa
      361 agctggtcgg tgctgtcagg catcggcgac ccggagcggg cgcagcaggc gatggcctcg
      421 ctggaggagc acctgatccg cccggcggag agcctggtgc tgctgctgac cccgccgttc
      481 gacaagtcgg atctcgatcc cggctacatc aagggctacg tgccgggcgt gcgcgagaac
      541 ggcggccagt acacccacgc cgccatctgg gtgggcttcg ccttcgccga actgcgcgac
      601 ggcgtacggg cgcatgccct gttcaacatg ctcaacccga tcaaccacgc gctcgacccc
      661 gacgaccgcg accgctacaa ggtcgagcca tacgtgatcg ccgccgatgt gtacgcccac
      721 ccgcagcacg tcgggcgggg cggctggacc tggtacaccg gctcggcggc ctggatgtac
      781 cgcctgggcg tggaagcgat cctgggtctg cgccgggtgg atggcggcct gtgcgtcgat
      841 ccggcgatcc cgccacattg gcccggcttc gaggtctgct accgccacgg cgcggccacc
      901 tacaccatcc aggtggacaa cacggcgggc ggcggccacg gcgtggtggc cgtgacactc
      961 gacggcgcgc cctgccccga cgggcgcatc ccgctcgacc cggcccccgg cacgcacgcg
     1021 gtcgaggtcc gcctcggccc cggcgaataa cgacggaagt tcaccgcgaa gggacgaagc
     1081 gcacgaaggg ttacgacgga tttcaccgcg aaggaacgaa ggacgcgaag gtttttaggg
     1141 tttccggcat ggtagcaggc gcgcgacccc gatcgccagg cccgaaaagc ccgtcgcgcc
     1201 ttcgcggcga accctccgtc gtcaaacccc caaaaaccct tcgcgtcctt tgttccttcg
     1261 cgatgaaatt cccggctcca ccgtgcaaaa cgcgcccgga tcggctatga tatagcagca
     1321 ctccgggcgg tggccggccc ctacccccgc cccatcccgg ctttagccat gaggcgctat
     1381 gcactacaat tcggttgacc actttctcac cgcccacgaa tcccagatta tcgacatgat
     1441 ggcgagcgcg caacaggcca ccggcgggca ctatgccggc ctgtccgaag ccgcgcgccg
     1501 ggccgatgcg caggccgccg cccagcgtat gatggccgcc gtacgccagg tgcagttgga
     1561 cggccagacc gtgcaggcga cggcacggca gcgcgacagc gcgggcatcc ccctggacga
     1621 cctcacgcgg ctggtggacc agatggaaac gcggttcatc gcctttgtgc aggcggaatt
     1681 gggcgcgcag ccggagctgg ccgccgagat cctgcgccgc ctgcgcaacg ccaacgcccg
     1741 cttccgcagc aacgtcatgg cggtcaagct cgacaagacg atccggcaac tccagcggcg
     1801 ctaacgggcc gcgtgttcac cgcacgggcc gaccctcagc gaacgcgatc gaagaacggg
     1861 gtctggccct gttccaccgc ggccagcgca tagagataca gggccgccga ccagacctgc
     1921 cagtcctccc cgcgtggggt gccgtcctga gcgcggatcc attcgttgaa gccaaaggcg
     1981 accgcgtgat tgcgcgccgg gcgcaccagg gcggccagcg cggcgagttt gcgcgcggcg
     2041 agcgcctgtt cgccggccgc caccagcgcg gccacataga agccgcagac gaagggccag
     2101 atgccgccgt tgtggtattc accgggctgg ttgaactcgg cgtagcgcgg gaaccaatcg
     2161 ggatcggcgg ggcggatgta ggggaagaga cagggcggca gatccagggc cagatcgccc
     2221 cgcgcgcgca gctctgcaca ctcggcttcg atccaggcga ccatggcgcg cgcccggtcg
     2281 ggggacgcga ggccggtcag gatggccagg ctgttgccca gcaggtcgaa gcgggtgctg
     2341 ccccagacct tgtaggccca gagcgcgtag tagggcgcat cgggcagcag caacccctcg
     2401 gcgccatgcg ggttgtcgtg agccaggagt tggatgggcc gatttatctg cgcggccagc
     2461 cccgccgcct gggcagcctc gccgtagagg cgcaggtagg cgtagaccag cgtgttgaca
     2521 tagaggccgt gccccagcac ccactgttcg tcgcgccagt cgctcgtcgg ctgctgggcc
     2581 accaggtagc cgtcgtcgtc gctctggtag gccatccagc gccgagcgcg ggcagcggcg
     2641 gcttccagga acgcggtctc gcccgtgacc cggcggacga gcgccaggcc gatcaggaac
     2701 agcggcgtgg tgtcactcga accgcggtcg gcgggggcgt cggccagacc ggggatctgg
     2761 ccgtgcggac tctggatggc ggccagggcg ctgagcatgc gccggatcgt gtccagcaac
     2821 aacgggtcgc ccgtcgtcag gatgcccagg gcggcgatca tccagtcgcg ggtgtagggt
     2881 tcggggtagc cccagcccgc cgtgcgcggc agctcaccgc gcgggccgcg ggcgcagtag
     2941 tgcaacacct ccagcgcggc ccggcgggcc tcctcgatta cccggtcgtc tgctgccata
     3001 tgccggctac agcaagccgc acgccagccg tgcgccccct acccttccgc atccatgttg
     3061 gtgaagaggt tgttgagcga ttcggccagg gtcgggtggg cgaaggtagc gtcgcggagc
     3121 acggtgtagg gcaagttgcc catcatggcg atctgcaact gggccatgat ttcgccgccc
     3181 tccaggccca gcacggcgca gccgaggatc tgcttggtgt cggcgtcg
//
LOCUS       DASUYC010000907         4652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1985176, whole genome shotgun sequence.
ACCESSION   DASUYC010000907 DASUYC010000000
VERSION     DASUYC010000907.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1985176"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..861
                     /locus_tag="VF276_14860"
     CDS             244..861
                     /locus_tag="VF276_14860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608182.1"
                     /translation="MMRWLIRLSIGAGVAALAIYLWSRLNQDYDDDEIEDEIPLEFDV
                     PMESAVSTLEAPIEFDVPMDSATASVDVPAEAPSRNGDTGVGPGADLDQSVAAPAPAM
                     PAPAAPTASDTATQAAPDTTAQATPAAPTAAGATGTEQHDDLTIIRGIGPVFQKRLNE
                     LGITTFRQLAGTKPAMLDAEGINGVGVDLESWIEQARELAEQQNT"
     gene            906..2057
                     /locus_tag="VF276_14865"
     CDS             906..2057
                     /locus_tag="VF276_14865"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX6608183.1"
                     /translation="MQILQTQSGIDRQAGALTAGPADVRARLARLHESSPAERAELRE
                     YLLEQITAPAMLMRAVAAEGLGRVGDERIIGPLIQALRDPAPLVQWRAAHALHMLHAR
                     ALVAPDRLVFQEGRPFEQWKSGVLDALMAQLRSGSTAERLDAAVSLAEIRVPTTLPAL
                     VAAAGDEQVAVRQEISRAILVVVDDDAALIAQARAALQLRLHDGDPLVRRAAIDLLGR
                     LGDRAAVPGLLPLLHDGDAGVRWHVCEALGLLGAPTASEGLISRLADDEPWVRRAAAD
                     ALGRLGDPYAVAPLVQISHDDSALVRRAVLEALGRIGSREGQPALLRGLRDRDPAARL
                     AAVTALAQMGDRQALKALAGLARDRTRLGTRQINREAAAATRKIKARSR"
     gene            2153..2587
                     /locus_tag="VF276_14870"
     CDS             2153..2587
                     /locus_tag="VF276_14870"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX6608184.1"
                     /translation="MATVVTSAGGIVYREAPGEPQILMIADRLGRWSFPKGMVQRGED
                     PAAAARREVQEETGIEGEILQLLGQTHYFFRQGGALIDKTVYFYLIRALTTAITPQLS
                     EVADARWFPAGEAIRNSAFHANTELLHKAIAALAEENGARPS"
     gene            2716..>4652
                     /locus_tag="VF276_14875"
     CDS             2716..>4652
                     /locus_tag="VF276_14875"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEX6608185.1"
                     /translation="MQTRCPLCHSWTLLPAHYPAGQPIHPLCDGCGAELPAVAYAHRY
                     DLCRVCLHRYLTDYGACPLCPPDADPATALAWQHLWLDHGTAPVVAPLSDRYCTALWQ
                     SYQQDQAGSAGRGSSGGGGRNSNGYTRNGNSAGKGHNTGARPAARPVALATAPVEAPP
                     AALSPNVAALLPAGPVALFEMEAPAPAAVNGNGHDGDSPAVAVRSDEGLSGVDYELIQ
                     DSARLVEVADLLGPEPLLGVDTETDGLDPYTSRLLLLQVATPQKAYIVDCQRVDPLPL
                     KPILESTRSLKILQNAKFDYKILKHIAGIELCNIFDTMLAERLLTAGRAREVSLRQLA
                     LKYAGIAMDKTIRKSFVGVKGDGYLSEEQLVYAARDALVMFEIQRAQQAELKKLRMTK
                     VAELEFQCVAAVGDMELAGVKIDTDHWRAILTRVAVDRDRAAEELNEMLAAVAKQATM
                     FGVPTINLNSGPQLIEVFARLGVELADTSEATLIKAGDHPAIKKLLEYRSHEKTLSAF
                     GEHLLALINERTGRLHPDFNQYGADTGRFSCTNPNVQQIPATSDFRSCFVAAEGYRLV
                     TCVAEGTRVATERGLLKIESLVAGDQALQENGRLQPVQRVINQGERETVTLKTAMGYE
                     ITVTPEHRIRVLNEDGNYVWRA"
BASE COUNT          838 a         1523 c         1520 g          771 t
ORIGIN      
        1 gaaacattgt ttgtagcagt cgcggccaac aagaggaggc agtactcact gtgggcttcc
       61 tgtacttcta acggcgcttg tgtataatac agtgcagtga tggcgaccgg cacaacctgt
      121 ttcacgtgaa acacgccgga aatcatgaaa tgctaacgat ttactattac gtttttgctg
      181 gtatgttcct tgctcctacg ctgccgggca accggtgttt gagctctagc gggaggtgtt
      241 cgtatgatgc gctggctaat tcggctgagt attggggcgg gcgtggccgc gcttgccatt
      301 tacctctggt cgcgcttgaa ccaggactac gatgacgacg agatcgaaga cgagatcccg
      361 ctcgaattcg acgtacccat ggaaagcgcg gtctcgacgc tggaagcgcc gattgagttc
      421 gatgtgccga tggatagcgc cacggccagc gtggatgtgc cggcggaggc gcccagccgg
      481 aacggcgata ccggcgtggg tcccggcgcc gacctggatc agtccgttgc ggcacccgcg
      541 ccggccatgc ctgctcctgc tgcccccacg gcgtccgaca ctgcaaccca ggcggcgccc
      601 gatacgactg cccaggcgac accggctgct cctaccgctg cgggcgctac cggcacggag
      661 caacatgatg atctcactat catccgcggg atcgggccgg tattccagaa gcggctgaac
      721 gaactgggga tcaccacgtt tcgccagttg gccggcacca aaccggcgat gctcgacgcg
      781 gaaggtatca acggggtggg tgtcgacctg gaaagctgga tcgagcaggc gcgcgaactg
      841 gccgagcaac agaacacctg attttatctg gcggacttcc ctcatcactc gtgtctggcg
      901 gcaatatgca gatactacaa acgcaatcgg gaatcgaccg ccaggctggc gcgctaaccg
      961 ccgggccggc ggatgttcgt gcccggctgg cgcgcttgca cgagtcgtca cccgccgagc
     1021 gggccgagtt gcgcgagtac ctgctggagc agatcacggc gcccgccatg ctgatgcgcg
     1081 ccgtggccgc ggaggggtta ggccgggtag gcgacgaacg gatcatcggt ccgctgatcc
     1141 aggcgctgcg cgatccggcc cccctggtgc aatggcgggc ggcacatgcc ttgcacatgc
     1201 tccacgcgcg cgccctggtg gcacccgacc ggctggtttt ccaggaggga cgcccgttcg
     1261 agcaatggaa atccggtgtg ctcgatgccc tcatggcgca gttgcgcagc ggcagtaccg
     1321 cggaacggtt ggacgccgcc gtatcgctag ccgagattcg cgttccgacc accttgccgg
     1381 ctctggtggc cgccgccggc gacgaacagg tggcggtgcg ccaggagatc agccgggcga
     1441 tcctggtggt ggtggatgac gatgcagcgc tgatcgccca ggcgcgggcg gccctccagc
     1501 tgcgactgca cgatggcgat cccctggtgc ggcgcgccgc tattgatctc ctgggacgcc
     1561 tcggcgaccg ggccgcggtg ccggggttgc tgccgttgct gcatgacggc gatgcgggcg
     1621 tgcgctggca tgtctgcgag gcgttgggct tgctgggcgc tcctacggcg tccgaggggc
     1681 tgattagccg gctggccgac gacgaaccct gggtgcgccg ggccgccgcc gatgcgctcg
     1741 ggcgcctggg cgacccctat gccgtggcgc cgctggtcca gatcagccac gacgacagcg
     1801 ccctggtgcg gcgggcggtc ctcgaagcgc tgggccggat cggcagccgc gagggccaac
     1861 ccgccttact gcggggcctg cgcgacaggg acccggcggc acggctggcc gccgtcaccg
     1921 ctctggccca gatgggcgac cggcaggccc tcaaggcgct ggccggcctg gcccgcgatc
     1981 gtacccggct gggtactcgg cagatcaacc gcgaggcggc ggcggcaacc cgcaaaatca
     2041 aggcgcggag ccggtaagtc gtgccgcaaa acgcgtaaat aatccactta tccagaagtt
     2101 atccaccaat ttgtgtataa ctgcggtctt aacggatggg atgagcagca ttatggcgac
     2161 ggttgtcaca tcagcgggcg ggattgttta tcgcgaggca ccgggcgagc cgcagatcct
     2221 catgatcgcc gaccgcctgg gccgctggag cttccccaag ggcatggtcc agcgcggcga
     2281 agacccggcc gccgccgccc ggcgcgaagt gcaggaggag accggtatcg agggcgagat
     2341 cctgcaactg ctcgggcaaa cccactattt cttccggcaa ggcggggcgc tgatcgacaa
     2401 gactgtctat ttctacctca tccgcgcgct gaccaccgcc atcacacccc aactgtccga
     2461 ggtggccgac gcccgctggt tccctgccgg ggaggccata cgcaacagcg ccttccacgc
     2521 caataccgag ctattgcata aagccatcgc cgccctggcc gaagagaacg gggcgagacc
     2581 ttcttagttg acataaacgg cagttgacca ggattgagca gttgacataa ctgagcaagg
     2641 cttttgccgg atggccggta atcccgtata atagaacaaa catcctgcat cgccattgtt
     2701 ccagggagac cgattatgca gacccgctgc cccctctgcc atagctggac tcttttaccc
     2761 gcccattatc cggccgggca gcccatccat ccgctttgtg acggctgcgg ggccgagcta
     2821 cccgccgtcg cctacgcgca ccgctatgac ctctgccggg tctgcctgca ccgctacctg
     2881 accgactatg gtgcctgccc gctctgcccg ccggacgccg atccggcgac ggccctcgcc
     2941 tggcagcatc tctggctcga ccacggcacc gcgcccgtgg tggcgccact ctcggaccgc
     3001 tattgcaccg cgctctggca aagctatcaa caggaccagg ccggctcggc ggggcggggg
     3061 tcgagcggcg ggggcgggcg caacagcaac ggctacaccc ggaacggcaa cagcgccggg
     3121 aaaggccaca acaccggggc gcgcccggcg gcccggcccg tggcgctcgc caccgcgccc
     3181 gtcgaggcgc cgcccgccgc gttgtcgccc aacgtggcgg cgctgctgcc cgcgggcccc
     3241 gtcgctttat tcgagatgga agcgcccgcg ccggcggcgg tgaacggcaa cggccatgat
     3301 ggagacagcc cggctgtggc cgtgcggagc gacgagggcc tgagcggggt cgactacgaa
     3361 ctcattcagg actccgcgcg cctggtcgaa gtcgccgacc tgctcggccc ggagccgctg
     3421 ctgggcgtcg acaccgagac cgacggcctc gatccctaca cctcgcgctt gctcttgcta
     3481 caggtcgcga ccccgcagaa ggcctacatc gtcgattgcc agcgcgtcga tccgctgccg
     3541 ctcaagccga ttctggagag cacgcgcagc ctgaagatct tgcagaacgc caagttcgac
     3601 tacaagattc tcaagcatat cgccgggatc gagctgtgca acatcttcga cacgatgctg
     3661 gccgagcgcc tgttgaccgc ggggcgcgcc cgcgaggtca gcctgcgcca gcttgcgctc
     3721 aagtacgccg gcatcgctat ggacaagacg atccgcaaga gctttgtggg cgtgaagggc
     3781 gacggctatc tgtcggaaga gcaactggtt tacgccgccc gcgacgcgct ggtcatgttc
     3841 gagatccagc gcgctcagca agccgagttg aaaaagctgc gtatgaccaa ggtggccgag
     3901 ttggagttcc agtgcgtggc cgccgtgggc gacatggagc tggccggcgt gaagatcgac
     3961 accgaccact ggcgggcgat cctgacccgc gtggccgtgg atcgcgaccg cgccgccgaa
     4021 gagttgaacg agatgctggc cgccgtcgcc aagcaagcca cgatgtttgg cgtgccgacg
     4081 atcaacctga acagtggtcc ccaactgatc gaggtcttcg cccggctcgg tgtcgaactg
     4141 gcggacacca gcgaagccac gctgatcaag gcgggcgacc acccggccat caaaaagctc
     4201 ctcgaatacc ggtcgcacga aaaaacgctt agcgcgttcg gtgagcacct gctggcgctg
     4261 attaacgagc gcaccggccg cctccacccc gacttcaacc agtacggcgc cgacaccggc
     4321 cgcttctcct gcaccaaccc caacgtgcag cagatcccgg ccacatccga cttccgctcc
     4381 tgtttcgtgg ccgccgaggg gtatagatta gtcacctgcg tagcggaagg tactcgcgtc
     4441 gccaccgagc gggggctcct taagatagag tcgctggtcg ccggggatca agcgctgcag
     4501 gaaaatggtc gattgcagcc cgttcagcgc gtgatcaatc aaggtgagcg cgagaccgtt
     4561 accctcaaga cggcgatggg ctatgaaata accgtcacgc ctgagcatcg catccgcgta
     4621 ctcaatgagg atgggaacta cgtctggcgg gc
//
LOCUS       DASUYC010000908         4561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_2035951, whole genome shotgun sequence.
ACCESSION   DASUYC010000908 DASUYC010000000
VERSION     DASUYC010000908.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4561
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2035951"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="VF276_14880"
     CDS             <1..821
                     /locus_tag="VF276_14880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HEX6608186.1"
                     /translation="YVAMIGDGVNDVLSLKRAQLAVAMQSGSQATRSVADIVLLDDSF
                     AILPAAVQEGRRIISGMQDVMRLVLAHTFYIVLVILFAAIVDVAFPTTPKLRSLITLL
                     SVGIPTMAIAAWARPDLPPGSVLRSVLHFVVPAAFTVSAVSFAVYLVYLVQTGDLGLA
                     QAALTTIALLCGLVLVVFVEPPTPAWVAGDALSGDWRPAGAALAMLGLYGVAMYVPLV
                     HDFYGLTPLPLADLLLLTGVTGAWALVVRTIWRTQLFERLLRLRGRTEPAGGAG"
     gene            complement(907..2766)
                     /locus_tag="VF276_14885"
     CDS             complement(907..2766)
                     /locus_tag="VF276_14885"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEX6608187.1"
                     /translation="MRHIVWQTGLRIAGAAIAGGCLFAGVWAVEAGPAPGRQTAGPEA
                     GAAPMSIAGAARPAGADAPAPAGPPVPEACVPLWGAVSSPGYGSLVGVAAAGANDVWA
                     ISDSTVLHWDGQGWAPTPTATPPGSPTPTPTPNLNAGYGARHYHGLAARASNDVWTSG
                     HVDAWPIYNPSAAFISHFDGTVWHDVYFGSPTRRETAPDGGSIIDSTFYAVAPVGDGE
                     AWAVGRRDNYGQPSGTLAAHCTPAGCDHVGATIDPGGYPPSPKTLRAVAGTGPDDVWA
                     VGANDYVSGGSLIVHWNGTAWAQVPAPDVGFLTGVAAPAPDDAWAVSSIAMLHWDGAA
                     WTVVPGVAGATAIAAHGGTDIWAVGAGIQHWDGQAWQTVAAPAGSYAAVSILATVEVW
                     AAGSGPGGTGLVARALPPPAFADVPVSQPFFTPIQTLACRNVISGYACGAPGEPCPGV
                     YYRPGATITRGQVAKIVALSAGLSAPPTRQQFADVPSGHPFYTWIGQLAGQGIISGYT
                     CGGPGEPCIPPADLPYFRPGAPVTRGQFAKIVALAAGYADSPTGQIFADVPPGQPFYR
                     WIEQVARRGIISGYTCGGPGEPCVPPDNRPYFRPAATASRGQVAKMAALAFFP"
     gene            complement(2891..4456)
                     /locus_tag="VF276_14890"
     CDS             complement(2891..4456)
                     /locus_tag="VF276_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6608188.1"
                     /translation="MTAAARAPGSAEAGAAAQGRWVLVGTILASSMAYIDGSALNVAL
                     PAVQAGLHATGPQLLWVINSYLLMLAALILIGGSLGDKRGRKRVFMAGIALFLLASLV
                     CGLAPSVEFLIAARLVQGIGGALMIPGSLAIITAYFGEDRLGDAIGTWAAAGTIVTIG
                     GPMLGGVLANAGLWRGVFLINLPLGLVALLVLFYKVPESRDTTASGAIDYPGAALATL
                     GLAGLTYGFIAAPDQGLAAPTTLIALLGGAGALVAFVVVEVRSSHPMVPLQLFRSHTF
                     SGTNLLTLFLYGALSIGTFLLSLNLVQVQGYSQALAGFVFLPFSVLLAGFSRWTGRLV
                     DRFGARLPLIAGPALVGVGFLLLGLVGLSAGPSAYWTSFFPGIVMLGLGMAVTVAPLT
                     TAVMGAVATHYAGTASGINNAVSRTAGVLAIAIVGAVALAVFAGVLDTHTAALPLGPE
                     ARAALRAETAQLGAAHVPPGVASDQAAAIALAIKLAFVDAFRVVMFICAGLAWLSGLM
                     AALLVEGRSRPAA"
BASE COUNT          675 a         1640 c         1631 g          615 t
ORIGIN      
        1 actatgtcgc catgatcggc gacggcgtca acgacgtact ctcgctgaag cgcgcgcaac
       61 tggccgtcgc gatgcagagc ggcagccagg cgacccgcag tgtggccgac atcgtgttgc
      121 tggacgactc gttcgccatc ctgcccgcag cggtgcagga gggacggcgc atcatcagcg
      181 ggatgcaaga cgtgatgcgg ctggtgctgg ctcacacgtt ctatatcgtg ctggtcatcc
      241 tcttcgccgc catcgtcgat gtggcctttc ccacgacgcc gaagctgcgt tcgctgatca
      301 ccttactcag cgtggggatc ccgacgatgg cgatcgcggc ctgggcccgg cccgacctcc
      361 cgcccggcag cgtcttgcgc tcggtgctcc acttcgtggt gcccgccgcg ttcacggtga
      421 gcgccgtcag ctttgccgtc tatctggtgt acctggtgca aacgggcgat ctcggcctgg
      481 cccaggctgc actgaccacc atcgcgctgc tgtgcggcct ggtgctggtc gtgtttgtcg
      541 agccacccac accggcctgg gtagcaggcg atgcgctcag cggcgattgg cgccccgccg
      601 gggcggcgct ggcgatgctg gggctctacg gtgtcgccat gtatgtgccg ctcgtgcacg
      661 acttctacgg gttgacgccc ctaccgctcg ccgatctcct gctgctgacc ggcgtgaccg
      721 gcgcgtgggc gcttgtcgtg cggacaatct ggcgcacgca gttattcgag cggctgctgc
      781 gcctgcgcgg gcggacggaa cccgccggcg gcgccgggta ggcgttctgg gctatagctc
      841 acccggcgcc gtggggccgg cggtgcctct ttagattcag gcagcccgcg cccgcgtgtg
      901 ccccgatcag gggaagaacg ccagggccgc catcttcgcg acctgcccgc ggctcgccgt
      961 ggccgcgggc cggaagtagg gccggttgtc gggcgggacg catggctcac ccggcccgcc
     1021 gcaggtgtag ccgctgatga ttccgcggcg cgccacctgc tcgatccagc ggtagaaggg
     1081 ctggccgggc ggcacatcgg cgaagatttg cccggtaggt gagtccgcgt agcctgccgc
     1141 cagggcgacg atcttggcga actggccgcg cgtgacgggc gcgccggggc ggaagtaggg
     1201 caggtcggcg ggggggatac acggctcgcc cggcccaccg cacgtgtagc cgctgataat
     1261 gccctggccg gccagttgcc cgatccaggt gtagaacggg tgaccggacg gcacgtcggc
     1321 gaactgctgg cgcgtgggcg gggcgctcaa ccccgcgctg agggcgacga tcttggcgac
     1381 ctggccgcgg gtgatcgtgg cgccgggccg gtaatagacg ccggggcagg gttcgccggg
     1441 cgcgccgcac gcatacccgc tgatgacgtt acggcaggcc agcgtctgga tgggcgtgaa
     1501 gaagggctgg ctcaccggga catcggcgaa ggccggcggc ggcagggcgc gggccaccag
     1561 cccggtcccg cccggcccgc tgcccgccgc ccagacctcc acggttgcca ggatcgagac
     1621 ggcggcatag ctgcccgcgg gcgcggccac ggtttgccag gcctgcccgt cccagtgttg
     1681 gatccccgcg ccgaccgccc agatgtccgt cccaccgtgc gccgcgatgg ccgtggcccc
     1741 ggcgacgccc ggcaccaccg tccaggcggc gccgtcccag tgcagcatgg cgatgctgct
     1801 cacggcccac gcgtcgtccg gcgccggagc cgccacgccg gtcaggaacc cgacatcggg
     1861 ggcgggcacc tgggcccagg ccgtgccgtt ccagtggacg atcagcgagc cgccgctcac
     1921 gtaatcattc gcccccaccg cccacacgtc gtccggcccc gtgccggcca cggcccgcag
     1981 cgtcttcggg ctgggggggt agccgccggg atcgatggtg gcgcccacat ggtcgcaccc
     2041 ggcgggcgtg cagtgggccg ccagcgtgcc ggacggctgg ccatagttgt cgcgccggcc
     2101 cacggcccag gcttccccat cgcccaccgg ggcgacggca tagaaggtcg agtcgatgat
     2161 cgacccaccg tcgggtgcgg tctcgcgccg cgtcgggctg ccgaaataga catcatgcca
     2221 cactgtgccg tcgaagtggg agataaacgc cgcgcttgga ttgtagatcg gccaggcatc
     2281 gacgtggccg gaggtccaca cgtcgttgga tgcgcgcgcg gccaggccgt ggtagtgccg
     2341 tgcaccatag ccggcattca gattgggcgt cggggtgggt gtgggcgagc cgggcggcgt
     2401 ggccgtgggc gtcggcgccc agccctggcc gtcccagtgc agcacagtgc tgtcgctgat
     2461 ggcccacacg tcgtttgcgc cggcggccgc cacgcccacg agcgagccgt agccggggct
     2521 agacacggcg ccccagaggg gaacgcacgc ctccggcacc ggggggccgg cgggcgcagg
     2581 ggcatccgcc cccgccgggc gcgccgcccc ggcgatactc atcggcgcgg cgcccgcctc
     2641 cggccccgcc gtttgccgtc ccggcgcggg gccggcctcc accgcccata ccccggcgaa
     2701 gaggcacccg cccgcgatgg ctgcgcccgc gatccgcagc ccggtctgcc acacaatgtg
     2761 tcgcatgtcg cacctcctgc gtcaattggc cgcctagctc ccgcccggct ccagactacg
     2821 cttgccgcgc accgccccgt cgggggatgc atattagcag ggcgccggga gccgcgcaac
     2881 cgcgtcgggc tcacgccgcc ggtcgggacc ggccctccac caggagcgcc gccatgaggc
     2941 cgctgagcca ggccaggccc gcgcagatga acatcaccac ccggaacgca tcgacgaacg
     3001 ccagcttgat cgccagggca atcgccgccg cctggtccga ggcgacgcct ggcggcacat
     3061 gggccgcgcc cagttgggcg gtctcggccc ggagcgccgc gcgagcctcc gggccgagcg
     3121 gcagggccgc ggtgtgcgta tccagcacgc ccgcgaacac ggccagcgcc accgcgccga
     3181 cgatggcgat cgccagcacc ccggcggtgc gcgacacggc gttgttgatg ccggaggcgg
     3241 tgcccgcgta gtgcgtggcg accgcgccca tgacggcggt ggtgagcggc gcgacagtca
     3301 cggccatgcc caggcccagc atgacgatgc cgggaaagaa gctggtccag taagcggatg
     3361 ggccggcgct gagtccgacc aggcccagca gcagaaagcc gactccgacc agggccgggc
     3421 ccgcgatcag cggcaggcgc gcgccgaacc ggtccaccag gcgcccggtc cagcgggaga
     3481 aaccggccag cagcacactg aagggcagga agacgaaccc ggccagcgcc tggctgtagc
     3541 cttgcacctg cacaaggttc aacgagagca ggaaagtgcc gatgctcagg gcgccataga
     3601 ggaacagggt cagcagattg gtgccgctga aggtgtgcga acggaacaat tgcagcggca
     3661 ccatcgggtg ggagctacgc acctcgacca ccacgaacgc taccagcgcg ccagccccac
     3721 ccagtaacgc gatcagcgtc gtcggggcgg ccaggccctg gtcgggcgcg gcgatgaagc
     3781 cgtaggtcag cccggcgagg ccgagcgtcg ccagcgcggc gccgggatag tcgatggcgc
     3841 ccgacgcggt ggtatcgcga ctctccggca ctttgtagaa cagcaccagc agcgcgacca
     3901 ggccaagcgg caggtttatg aggaagacgc cccgccacag cccggcgttc gccagcacgc
     3961 cgcccagcat ggggccgcca atcgtcacga tggtgcccgc cgccgcccac gtgccgatgg
     4021 catcgcccag ccggtcctcg ccaaagtagg cggtaataat cgccaggctg ccggggatca
     4081 tcaacgcgcc gccgatgccc tggacgagcc gggcggcgat cagaaactcg acgctgggcg
     4141 ccaggccgca gaccagcgag gccagcagga acagcgcgat gccggccatg aacacgcgct
     4201 tgcgcccgcg cttatcgcct agcgacccgc cgatcaggat cagcgcggcc agcatcaaga
     4261 ggtaactgtt gatcacccac agcagttgcg ggccggtggc gtgcagcccg gcctgcacgg
     4321 cgggcagcgc cacgttcagc gccgagccgt cgatataggc catgctcgat gccaggatcg
     4381 tcccgaccag cacccagcgg ccctgcgccg ctgcgcctgc ctccgcgctg cccggcgccc
     4441 gcgctgccgc ggtcatgccg ccttcatcct cgccgggcgg ccgcgaccgc ttcgccgcgg
     4501 gtgtgggcgc cgatcttggc caggatggcc gacacatggt ggtcgaccgt cttcgcggag
     4561 a
//
LOCUS       DASUYC010000909         4472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_844906,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000909 DASUYC010000000
VERSION     DASUYC010000909.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844906"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            278..1444
                     /locus_tag="VF276_14895"
     CDS             278..1444
                     /locus_tag="VF276_14895"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX6608189.1"
                     /translation="MHYAHTSEAARSLSLTTGTTVSHSSRHLRRTLLTWGLALAFALA
                     LIAPLATVPSRAKAAPLPAPANASEVLTFPATGHSIKSAAFVRFFQAYGGIQTFGYPL
                     TEEMTENGRTVQYFERQRFEYHAENAGSDFEVLLGRLGDESSRARQPFGTVAPFADSG
                     TRVYIAQTHHALAEPFLSYWRSHGGVRILGYPISEVVTEGGMQVQYFERARFERHPEN
                     AGSPYEVLLTQLGKAAMEQRGLSAAPAAPAAPPAPQINGLEDSLLRQINAARTQAGLS
                     AVPLDSRLITLARDRSADMANRGYFDHHTPDGKTFLDMLKARGVPFTMAGEIIAENNY
                     PAAQTADQAYQGFMNSSEHHRIIMMSNWTAAGVGQAVDGKGMYYYTVIFSQGTR"
     gene            complement(1747..3981)
                     /locus_tag="VF276_14900"
     CDS             complement(1747..3981)
                     /locus_tag="VF276_14900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX6608190.1"
                     /translation="MSTDLDPDIQSRISLNGDGDLDFVPDAPPRRGRRLALIAGAAVL
                     LLILGAGTARLLAAPPTLNTVPVVRGDIRSTVETNGRLEALTSAQVAFKTSGRLVRVR
                     VHEGDTVAAGAVLAEQDTAALDRQLRQAQTDVTIAQLKLQQAKEGPRPEELAAAEADL
                     SSALAALDQLKSGGQAADIRAAQAALAQAVAKRDEVKRGATAAQIASAEATLREAQAG
                     LTKARAGAGPNDIAAAEAAVRQAQAGLDRAKQPARPEDIAAAEAAVREAQAGLDKVKA
                     GPTAADLAAAQARLDQAKANRNQVSATTSNAKEQARLQMEQAANAVRNAQDHYGTIAH
                     NNDEFYRNRGKKVPDDAQQAEDAARRAMEDAQNGLQQAQLAYEAAKQNEIAGLGQADA
                     AIQEAQAALDKLKAGSTAADLAAAQSGVDQAQANLAKLQAGPSAEDVAAAQAAVDQAQ
                     ANLAKLQAGPTAEDLAAAQASVDRALAALNELRAGPTTESLREAQAAVDQAQANLDKV
                     KQGGTSADRAAAEARVAAAQAALDLKKQGPTATDIALLEQQVQLAQYEVENATAALAD
                     ARLTAPFAGTVLTIDAKEGELVGGGAPVVELARLATLRVRADVDELDVGRVQPGQPVT
                     VTLDAYPGEKLPGSIETVAPGATQQQGSTVYAATVVFTPTAGVTPRPGMAAGLLITAQ
                     HKRDVLLVPNRALEQIGANRFVTIVEGDTQRKQRVEIGLSNADSTEIVDDGSLTAGQQ
                     VLVH"
     gene            complement(4044..>4472)
                     /locus_tag="VF276_14905"
     CDS             complement(4044..>4472)
                     /locus_tag="VF276_14905"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6608191.1"
                     /translation="KASSKEASQILSYLLYAMVVIVGVIGGIGIANTLTLNVLERRRE
                     IGVMRAIGAGNSHLVQAFLTEALFLGGAGYLLGLALGVPLARLLVFALSAVLFEISFH
                     FPAESILDAGLFTLALTAVASVGPALGAARMRIREALRYE"
BASE COUNT          597 a         1544 c         1580 g          751 t
ORIGIN      
        1 gttatagccc acatttgcta tagactcgaa ccctccaggt ttgctaccat ccctccataa
       61 acgccatgcc gccggcccgc ccgatacctg ctgggcgggt ggtcacaata agatataaaa
      121 gaggcttctt ctatagacag ggcccaggcc gtttgggagt ataaatccag cctgaccggg
      181 tccgcgcacc tggcggctca gcagcccgcc gtgctgctgt tcgcgcagtc cctcgtcgct
      241 cgctgcccga agctcgacga gtgttaaagg agatcccgtg cactacgccc acacgtccga
      301 agccgctcga tcgctctctc ttaccaccgg cacaactgta tcgcattcat cccggcacct
      361 gcgccggact ctgctgacct ggggcctggc tctggccttt gcgctggcgc tgatcgcgcc
      421 cctggcgacc gtgcccagcc gggccaaggc cgccccgctg ccggccccgg ccaacgcgag
      481 cgaggtgttg accttcccgg ccaccggcca ttcgatcaag agcgcagcgt ttgtgcggtt
      541 cttccaggcc tacggcggga ttcagacctt cggctatccg ctgaccgagg aaatgacgga
      601 gaatgggcgc acggtgcaat atttcgagcg ccagcgtttt gagtaccatg ccgaaaatgc
      661 cggcagtgat tttgaggtgc tgctgggccg cctgggcgac gagtccagcc gtgcccggca
      721 gccgttcggc acggtggcgc ccttcgccga ctccggcacg cgggtctata tcgcgcagac
      781 ccatcacgcg ctggccgagc cgttcctgag ctactggcgc agccacggcg gcgtgcgcat
      841 cctgggctat cccatctccg aggtcgtcac cgagggcggg atgcaggtgc agtacttcga
      901 gcgggcccgc ttcgagcgcc acccggagaa cgccggcagc ccctatgaag tgctgctgac
      961 ccagttgggc aaagccgcga tggaacagcg cgggctgtcg gcggcgcccg cggcccccgc
     1021 tgcgccgccc gccccgcaga tcaacgggct ggaagacagc ctgctgcgcc agatcaacgc
     1081 ggcgcgcacc caggccgggc tcagcgccgt gccgctcgac agccgcctga tcacgctggc
     1141 ccgcgaccgc tccgccgata tggcgaaccg cggctatttc gatcaccata cgcctgacgg
     1201 caagaccttc ctcgacatgc tgaaggcgcg cggggtgccg ttcaccatgg cgggcgagat
     1261 tatcgccgag aacaactacc ccgccgccca gaccgccgat caagcctacc agggcttcat
     1321 gaacagcagc gagcaccacc gcatcatcat gatgagcaac tggacggcgg cgggcgtcgg
     1381 ccaggcggtg gacggcaagg ggatgtacta ctacacggtc atcttctcgc aaggcacgcg
     1441 ctaacccacc acggcacaga cagggcgccg ggatcgcgat cccggcgccc tcgctttttg
     1501 cctcctgcac cgatccccgt ctggatcgac ccagatgcgc gtcttcggga acatcccggt
     1561 ggggcggcag gcgcgataaa tcgcgccgct acgaattacg gcggcatcca ggtataagag
     1621 cgactcatca agccagcctt cgtagcggcg cgatttatcg cgcctgccgc cttaccctga
     1681 tattaggggt aaaccagccc ggctaatgca cgccggccta tcgccctacc gccatcggcc
     1741 catcagctaa tgcaccagca cctgttgccc ggcggtcagg ctgccgtcgt ccacgatctc
     1801 cgtgctgtcg gcgttggaga ggccgatttc gacgcgctgc ttgcgttgcg tgtcgccctc
     1861 cacgatggtg acgaagcggt tggcgccgat ctgttcgagg gcgcggttgg gcaccagcag
     1921 cacgtcgcgt ttgtgctggg cggtgatgag caggccggcg gccatgccgg ggcgcggcgt
     1981 gaccccggcg gtgggggtga agaccaccgt ggcggcgtag accgtgctgc cctgctgctg
     2041 ggtggcgccg ggggccaccg tctcgatgct gcccggcagc ttctcgccgg ggtaggcgtc
     2101 gagcgtgacc gtgacgggct ggcccggctg cacgcgaccc acgtcgagtt cgtcgacgtc
     2161 ggcgcgcacg cgcagggtgg cgaggcgggc cagttccacg accggcgcgc ccccgcccac
     2221 cagttcgccc tccttggcgt cgatggtgag cacggtcccg gcgaagggcg cggtcaggcg
     2281 agcatcggcc agcgcggcgg tcgcgttctc gacttcgtat tgtgccagtt gcacctgctg
     2341 ttccagcagc gcgatgtcgg tggccgtagg gccttgcttt ttgaggtcga gcgcggcctg
     2401 ggcggcggcc acgcgcgctt ccgcggcggc ccggtcggcg gacgtgccgc cttgcttgac
     2461 cttgtcgagg ttggcctggg cctggtccac ggcggcctgc gcctcgcgca acgactcggt
     2521 ggtggggccg gcgcggagtt cgttgagcgc ggccagggcg cggtccacgc tcgcctgcgc
     2581 ggcggccagg tcttcggcag tcggcccggc ctgcagcttg gcgagattgg cctgcgcctg
     2641 gtccacggcg gcctgggcgg cggccacgtc ctccgcgctg ggcccggcct gcaacttggc
     2701 gaggttggcc tgcgcctggt ccacgcccga ttgcgcggcg gccaggtcgg cggcggtgga
     2761 gccggccttc agtttatcga gcgcggcctg cgcctcctgg atggcggcgt cggcctggcc
     2821 cagcccggcg atctcgttct gcttggccgc ctcgtaggcc agttgcgcct gttgcagccc
     2881 gttctgcgca tcctccatcg cgcgccgggc ggcgtcttcg gcctgctggg cgtcgtcggg
     2941 caccttcttg ccgcggttgc ggtagaactc gtcgttgtta tgggcgatgg tgccgtagtg
     3001 gtcctgggcg ttgcgcacgg cgttggcggc ctgctccatt tgcaggcggg cctgctcttt
     3061 ggcgttcgac gtggtggccg agacctggtt gcggttggcc ttggcctgat cgaggcgggc
     3121 ctgggcggcg gcgaggtcgg cggcggtggg gccggccttc accttgtcca gcccggcctg
     3181 cgcctcgcgg acggcggctt cggcggcggc gatgtcttcg gggcgggcgg gctgtttggc
     3241 ccggtcgagc ccggcctggg cctggcgcac ggcggcttcg gcggcggcga tgtcgttggg
     3301 gccggcgccc gcccgcgcct tggtcagccc ggcctgggcc tcgcgcaggg tggcttcggc
     3361 gctcgcgatc tgcgcggcgg tggccccgcg cttgacctcg tcacgcttgg cgacggcctg
     3421 ggccagcgcg gcctgcgcgg cgcggatgtc cgccgcctgg ccgccgctct tgagctggtc
     3481 gagcgcggcc agggcgctgc tcaggtcggc ctccgccgcg gccagttcct ccgggcgcgg
     3541 gccttccttc gcctgttgca gcttgagttg ggcaatcgtg acatcggttt gcgcctgccg
     3601 cagttggcgg tccagcgcgg cggtgtcctg ctcagccagc accgccccgg cggccaccgt
     3661 gtcgccttcg tggacccgca cgcgcaccag gcgcccgctg gttttgaaag ccacctgcgc
     3721 cgaggtgagt gcctccaggc gcccgttggt ctcgaccgtg gaccggatgt cgccgcgcac
     3781 caccggcacc gtgttgagcg tgggcggcgc ggccagcagc cgggccgtgc ccgcgccgag
     3841 gatcagcagc agcacggcgg cgcccgcgat cagcgcgagg cggcgcccgc ggcggggcgg
     3901 cgcatcgggc acgaagtcca ggtcgccgtc gccgttcagc gagatgcggg actggatgtc
     3961 ggggtcaagg tcggtgctca ccgggggatc ctttccagag gccggggcgg gatagcgggc
     4021 gcccggccca ggttgtacgc aggttattcg tagcgcagcg cttcgcggat gcgcatgcgc
     4081 gcggcgccga gcgcggggcc gacgctggcg acggcggtca gcgccagggt gaacagcccc
     4141 gcatccagta tactctctgc cgggaaatgg aacgagatct cgaacagcac ggcgctgagg
     4201 gcgaagacga gcaggcgcgc cagcggcacg ccgagggcca gccccagcag ataacccgcg
     4261 ccaccgagga acagggcctc ggtcaggaag gcttgcacca ggtggctgtt gcccgcgccg
     4321 atggcccgca tcacgccgat ctcgcggcgg cgttcgagca cgttcagcgt cagcgtgttg
     4381 gcgatgccga tgccgccgat gaccccgacg atgaccacca tcgcatagag caggtaggag
     4441 aggatctggc tggcctcttt gctgctcgcc tt
//
LOCUS       DASUYC010000910         2580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_669443,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000910 DASUYC010000000
VERSION     DASUYC010000910.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669443"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(349..1920)
                     /locus_tag="VF276_14910"
     CDS             complement(349..1920)
                     /locus_tag="VF276_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6608192.1"
                     /translation="MRLPALNPGPGPAPPAAVTSKEGELETLVRARYPLIYIVAWEER
                     RVETLLHSIAARRDKRLFVWTCTEGVQACDGTGQMVPADPAARDPLQALDFVQNCREA
                     AIFLLKDFHPYIDDTRPTPGTPVVTRKLRDLARVLKESHKTLVLLSPVLRFPAELEKD
                     ITVVNYGLPSLEELDQSLNVVINSVERRSALRLGLDPQTRERVLKAAQGLTLAEAENA
                     FARSLIVTRRLDVDLVIAEKEQLIRRSRILEYYRAPEVFEDVGGMDELKAWLRTRSLA
                     FSASAREFGLPEPRGILLLGVQGGGKSLVAKAVASLWQLPLIKLDLGKVFSELVGSSE
                     ENMRAALATAESIAPAILWLDELDKGLAGVASSHRSDAGTAARVFGSFLTWMQEKTSP
                     VFVIATANNVGSLPPEALRKGRFDEIFFVDLPTEQERREIFAIHILKRRRDPELFDLD
                     RLAAAAAGLSGAEIEQAVIGGLYDAFEAGRDLTNADILHSLQGSIPLSQTMREEIGAL
                     RDWARTRARPASRRR"
     gene            2259..>2580
                     /locus_tag="VF276_14915"
     CDS             2259..>2580
                     /locus_tag="VF276_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608193.1"
                     /translation="MKYGILVLSALLLLGIGLASSRPAWADGPAQAGTPAAVGTPVGG
                     PITVTNVVPANMVGREPNDLVGYFPAIFLSLGILGAGIAALVSIRRDRQKAQRMRGPD
                     APGAR"
BASE COUNT          434 a          864 c          854 g          428 t
ORIGIN      
        1 atgccggcgg ccagcacggc gcgctccagc acagcgggac ctttatagac gatgacgcta
       61 gcttgtgctt ccatggttag ctatcagctt tcagccgtca gctttcaggt attaaccgtc
      121 agcgacgaga cccgatcttg agcgacaagc atccggccgc cggatgctct attgaatacc
      181 attcgctatc agccccctga ccggccacgg tttcgtgggg ccggcgggcc ggtttgcaac
      241 cggcccctac atcgcaaaat cacaacggtc gcctgttagt ccgccatcag tcaactcaac
      301 tacatacctt cttacaaaag aacctgacgg ctgaaagctg acggctcgtt accttcgccg
      361 cgacgccggg cgggcgcggg tgcgcgccca gtcgcgcagg gcgccgatct cctcgcgcat
      421 ggtctgcgag aggggaatgg aaccttgcag cgaatggaga atatccgcgt tggtcaggtc
      481 gcggcccgcc tcgaaggcgt cgtaaaggcc gccgatgacc gcctgctcga tttccgcgcc
      541 gctcagcccg gcggcggccg cggccagccg gtccaggtcg aataactcgg gatcgcggcg
      601 gcgcttcagg atatggatgg cgaagatctc gcgtcgctcc tgctcggtgg gcaggtccac
      661 gaagaagatc tcgtcgaagc gccccttgcg caacgcctcc ggcggcaggc tgccgacgtt
      721 gttggccgtg gcgatgacga acacgggcga ggtcttctcc tgcatccagg tcaggaacga
      781 gccgaacacg cgcgccgccg tgcccgcgtc cgaccggtgc gacgaggcca cgccggccag
      841 tcccttgtcc agctcgtcga gccagaggat ggccggggcg atgctctccg ccgtcgccag
      901 ggccgcgcgc atgttctcct cggacgagcc caccagttcg ctgaacacct tgcccaggtc
      961 gagcttgatc aacggcaact gccacaggct ggcgaccgcc ttggcgacca ggctcttgcc
     1021 gccgccctgc acccccagca gcaggatgcc gcgtggctcc ggcaggccga actcgcgggc
     1081 gctggcgctg aaggccaggg agcgggtgcg cagccacgcc ttcaactcgt ccatgccgcc
     1141 cacatcctcg aacacctcgg gtgcgcgata gtactccagg atgcgcgagc ggcggatcag
     1201 ttgctccttc tcggcgatca ccaggtccac atccaggcgg cgggtcacga tgagcgagcg
     1261 ggcaaaggcg ttctcggcct cggccagggt cagcccctgc gccgccttga gcacgcgctc
     1321 ccgcgtctgc gggtccagcc ccaggcgcag cgccgagcgc cgctccaccg agttgatcac
     1381 cacgttcagc gactggtcta actcctccag ggagggcagg ccgtagttga ccacggtgat
     1441 atccttttcc aactcggcgg ggaagcgcag caccggcgac agcagcacca gcgtcttgtg
     1501 gctctccttg agcacgcggg ccaggtcgcg cagcttgcgc gtgaccacgg gcgtgccggg
     1561 cgtggggcgg gtatcgtcga tgtaggggtg gaagtccttg agcaggaaga tggcggcttc
     1621 gcggcagttc tgcacgaagt cgagcgcctg gagcgggtcg cgggccgcgg ggtccgcggg
     1681 caccatctgg ccggtgccgt cacacgcctg cacgccctcg gtgcacgtcc acacgaacag
     1741 gcgcttatcc cgccgggctg caatcgaatg cagcagcgtt tcaacccggc gctcttccca
     1801 ggcgacgata tagatcagtg gataacgggc acgaacaagc gtctccagct cgccttcttt
     1861 gctggtgacg gcagcaggag gggcaggtcc ggggccaggg tttaatgcag gtagacgcat
     1921 gcgcgctcct ttgagtgtag tgctattgta gcaccccact ccgcttatgt caataggaac
     1981 gcaaagcagc ccagaagcgc gcgcagacgc ccggcggacg cgcccggagg ctcaagcctc
     2041 cggctaccgc aacgaaggcc gcctgcgcgg cctggcgcat cgcggggtgc caggtaaccg
     2101 cttgcgcccc ggcgggcgag atggtatagt agtcgccggt atgcgcttgt gcaggcggga
     2161 ttgcgcctgc gctcccgcgc cgctggcata acacttgccc ctactccggt attatcggga
     2221 gacacaagat ccgattattt tttggggagg ctaccggcat gaaatacggc atcctggtcc
     2281 tgagcgcgct gttgctgctg gggatcggac tggcaagcag ccgcccggca tgggcggacg
     2341 gtccggcgca agcgggaaca cccgcggccg tgggcacgcc ggttggcggc cccatcacgg
     2401 tgacgaatgt ggttcccgcc aacatggtgg gccgggaacc gaacgatctg gtcggctact
     2461 tcccggcgat cttcctgtcg ctcggcatcc tcggcgcggg gatcgctgcg ctggtgtcca
     2521 tccggcggga cagacagaag gcgcagcgca tgcgcggccc cgatgccccc ggcgcgcgcc
//
LOCUS       DASUYC010000911         2576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_844995,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000911 DASUYC010000000
VERSION     DASUYC010000911.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844995"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(284..688)
                     /locus_tag="VF276_14920"
     CDS             complement(284..688)
                     /locus_tag="VF276_14920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608194.1"
                     /translation="MDQHQPGAEPAEQTTGARATSPFDGEAVLDAVHVHTFTADPIEL
                     YRNERSTIRWEIQRPPGPGVNLRFFIRDDQTGNLRGLASDSGSQTIQPAGLGRFQYSL
                     WGQIGSVSGQVGAERGRLYLTVLPLRNPQGET"
     gene            complement(821..2143)
                     /locus_tag="VF276_14925"
     CDS             complement(821..2143)
                     /locus_tag="VF276_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6608195.1"
                     /translation="MAQELLLAIPPVRSTEQTTCCVVGAGPAGVMLAFMLARQGIPVI
                     LLESHLDFDRDFRGDTLHPSILEVLDELGLAGRLLQLPHTELSLITPPGGGPQFDLRE
                     LKTRFPFVAMMPQTRFLTFITHEAQRFPTFRLVLGARVEALITEAGQVRGVRYRAPDG
                     WHEVRALLTVGTDGRFSRLRKLAGFVPIASSPPMDVLWLRLARRAADPHGLMARFGGG
                     RALIFLEREDHWQIGYIIGKGTYQEVRAAGLEALRAAIAESAPDFADRVGELQDWKQV
                     AMLSVEANRLRRWYRPGLLLLGDAAHVMSPAGGNGINYAVMDAVAAANILSAPLRAGR
                     VAVADLARVQRRRAWPTRVIQAIVNVMQDRLLKAALDPSSSFVFPRFLGWPLVRRLIP
                     QFFGFGIRPEHVQPQTPRRRGLLRRRGSKGGRRTSRRTGTLSPADRDQ"
     gene            complement(2208..>2576)
                     /locus_tag="VF276_14930"
     CDS             complement(2208..>2576)
                     /locus_tag="VF276_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013866151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HEX6608196.1"
                     /translation="MEPGAILVCPTTTPAWTPLFAQARGLVTDIGGILAHGSIVAREY
                     GIPAVMGTGTATERIRPQQVISVDGDRGVVELGGADPDTSVPERKGRALGFALVLAAA
                     GSLLLWRQQHRRARRLSGVA"
BASE COUNT          448 a          866 c          856 g          406 t
ORIGIN      
        1 attatagatc aagtgggcgc cggcgacgct ggttgttcgt gatctcgtgc ttgcactggg
       61 ggagcggggc ctccgcgctc ccccggtgca agcaccagcc gcccggcagc gccggcccgg
      121 ctctcttccc cagttcggta agaaaggaac cggtccgcag cgcccagggg gccaccgcta
      181 tcaacgcttc cagcagatcc tttggcttta gcacaggtct ggaggttagc ctcgtaggcg
      241 gtaacctcca gcgtcgagtc gcctcgccct cctgcccagg tggttaggtc tccccttgcg
      301 ggttgcgcag ggggagcacc gtgagataga gccggccgcg ttccgcgccc acctgcccgc
      361 tgaccgaccc gatctggccc cacaaactgt actggaagcg ccccaggcct gccggttgga
      421 tcgtctggct gccgctgtcg gaggcgaggc cccgcaaatt gccggtctgg tcatcgcgga
      481 tgaagaaccg caggttcacc cctggaccgg gaggtcgctg gatttcccag cggatcgtgc
      541 tgcgctcgtt gcggtatagt tcgatcggat cggcggtgaa ggtatggaca tgcacggcgt
      601 ccaggacggc ctcgccgtcg aacggagagg ttgcacgggc gccggtggtc tgttctgccg
      661 gctccgcgcc gggctgatgc tgatccatga gaaacacgct cctttcgata aaccacggtg
      721 aattctcggc caaccgagcg tagataagct tcgctctttg ctacatatac agcctgccga
      781 ccccaggcag catgaggcgg caaacccgac cgcgctggga ttattgatcc cggtcggcag
      841 gggacagcgt tccggtccgg cggctggtgc ggcgcccccc tttgctaccc cgccgccgca
      901 acaacccacg ccggcgaggc gtctgcggct gcacatgctc gggccggatg ccaaagccaa
      961 agaactgcgg gatcagccgg cgcaccagcg gccagcccag gaagcggggg aagacaaagc
     1021 tgctgctcgg gtcaagggcg gctttgagca gccgatcttg catgacattg acgatcgcct
     1081 ggatgacccg ggtcggccat gcgcgccgcc gttggacccg ggccagatcc gccaccgcca
     1141 cccgcccggc gcgcagcggc gcactgagga tattggccgc cgcgacggcg tccataaccg
     1201 catagttaat cccgttgccg ccggccgggg acatgacatg ggccgcatcg cccagcagga
     1261 gcaggcccgg ccggtaccag cgccgcagcc ggttggcttc caccgagagc atcgccacct
     1321 gtttccagtc ctgcagttcg cccacccgat ccgcaaagtc gggcgcgctc tcggcaatgg
     1381 ccgcgcgcag ggcctccagg ccggcggccc gcacctcctg ataggtgcct ttgccgatga
     1441 tgtagccgat ctgccagtga tcttcgcgtt ccaggaagat cagcgcccgc cccccgccga
     1501 atcgagccat caggccatgg gggtccgcgg cccggcgcgc gagccgcaac cagagcacgt
     1561 ccatcggtgg cgaggaggca atcgggacaa acccggccag tttgcgcaac cgggagaaac
     1621 gcccatcggt ccccacggtg aggagcgccc gcacctcatg ccagccatcc ggcgcgcggt
     1681 agcgcacgcc ccggacctgg cccgcttcgg taatcaaggc ttccacgcgc gcgcccagca
     1741 ccagccgaaa ggtcgggaag cgctgtgcct catgggtgat gaaggtcaga aagcgcgtct
     1801 gcggcatcat cgcgacaaag gggaacctgg tcttcaactc ccgcagatcg aactgcggcc
     1861 ccccgcccgg cggcgtgatc aggctcaatt cggtgtgggg taactggagc agccggccgg
     1921 ccagcccgag ttcgtccagt acctccagga tagaggggtg cagggtatcg ccgcgaaaat
     1981 cgcggtcaaa gtccaggtgc gattcgagca ggatgaccgg gattccctgc cgcgccagca
     2041 tgaacgcgag catgaccccg gctgggccgg cgccgacgac gcagcaggtg gtctgctcgg
     2101 ttgaccgaac ggggggtatg gctaggagaa gctcttgggc catatcagga cctccattat
     2161 tgttccctgc gagaagtcag tcagatcgca gttgtcccct gattgtgtta agctacaccg
     2221 ctgaggcggc gagcacggcg gtgctgctgg cgccagagca ggagactgcc ggcagccgcc
     2281 agaaccaacg cgaacccgag tgcccgcccc ttccgttcgg gtacgctggt gtccgggtcc
     2341 gccccgccca gctcaaccac cccccgatca ccgtcaaccg agatgacctg ctgaggacgg
     2401 atgcgctcgg tggcagtccc ggtgcccatg accgcgggga tgccgtactc gcgggcgaca
     2461 atcgagccgt gggccaggat gccgccgatg tcggtgacga gaccgcgggc ctgggcaaag
     2521 agtggagtcc aggcgggggt ggtcgtcggg cagacgagga tggcacctgg ctccat
//
LOCUS       DASUYC010000912         4423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1305339, whole genome shotgun sequence.
ACCESSION   DASUYC010000912 DASUYC010000000
VERSION     DASUYC010000912.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305339"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..420)
                     /locus_tag="VF276_14935"
     CDS             complement(223..420)
                     /locus_tag="VF276_14935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608197.1"
                     /translation="MGKTARRAFILRAIFGLLVALGPVVEIVLGAFLAPAHYAPPAHP
                     RTWIPLYILLGKMERAPTFPA"
     gene            complement(777..1820)
                     /locus_tag="VF276_14940"
     CDS             complement(777..1820)
                     /locus_tag="VF276_14940"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEX6608198.1"
                     /translation="MSSLLATSVSFAPTAAAAGVGPNKPAVVTNTGGGGVRFREGAGV
                     GYDVKGTLGEGDKVQVLEGPVKDGAGHSWWRVTVNSNGRIGWVDTRYLGPAGSSSSSG
                     SKATTSSKTTTAKGPATLKGYAKVATPGGAALRLRSTPGGTIKSTAGQGTVFSIVKGP
                     TKDKNGDSWYQVSNANVTGWASATYLVSAPAPKKTTQATAKKSTTTTARTGTARGTTQ
                     PPAARSSAGGVVSVAMNYVGYAYRYGGTSPSGFDCSGFVYYVFNKAGIGLGRAMSSQI
                     AAGPHISSSQLQPGDLVYFANTYRRGISHIAIYAGNGKIVHAADYGIGVTVSNLWSPY
                     WTAHYAGATRVAR"
     gene            complement(2748..3425)
                     /locus_tag="VF276_14945"
     CDS             complement(2748..3425)
                     /locus_tag="VF276_14945"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX6608199.1"
                     /translation="MIFLLGSLLLLGLLAWVTYQSGKLLRVVPVQENLLLAPVENAVK
                     AGFIAVCVGLAAISGLPPDQFGWTFTHPLQIAALGIIFGLAAQIVVNLITHWAIGRFG
                     KGVYSPVVMKNVFPRTRREWLLVPAAMLLAVLLEEVLFRGLLLGGFSVLIPPPILIAV
                     LSVIFGIMHSPQGTLGMIVAAGIGAGLSVLFLATGSLLAPLVAHYLINLLQLIRAREE
                     WRWLQEY"
     gene            3585..>4423
                     /locus_tag="VF276_14950"
     CDS             3585..>4423
                     /locus_tag="VF276_14950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608200.1"
                     /translation="MDERRGRWAAVGALAGVALVLAVAGALLPQGVFWHSDEGAKFLQ
                     MVNLRWGPDGLDTSIYYPGRALDPLLQFVPFHPKQYHSDAAGQIYLQWPIFLALFTRP
                     LYDLLGWAGLYVLPWAGALAACWLTYRLARAVGAPPGWAWVAIPLLGLTTPLGFYSWV
                     FFEHTLAAALVAGAALLIVRGLPQQDWRRLAGAGALVGAGIYLRSELYILVLAAGGLV
                     AVRAFAALLPGDDRRPALDRAAAWRVVRLGGAGLVGLLIVLLPLWGYYAASAGGPLPQ
                     HAT"
BASE COUNT          770 a         1387 c         1534 g          732 t
ORIGIN      
        1 gccgcccagg tcagactcag agacgggctc gcggcaccag cgaagcacga ccttggacaa
       61 gttacccgcc tgccccacag gaaacggatt ttcatacgcg ctggtgtgcc gtaggcacat
      121 gattggctca gagtgacaag gattcgtgac gcggggatta agctgcgaga gtcggcgaga
      181 ctcttcgcgg cgctcagagt gacaagggcg gtgtcgcggg gattaagccg ggaaggttgg
      241 ggcacgctcc attttgccga ggagaatata gagcgggatc caggtgcggg gatgcgcggg
      301 cggcgcatag tgcgcgggcg cgaggaaggc gcccagcacg atctcgacca cagggccgag
      361 cgctacgagc agaccaaaga tggcccggag aatgaaggcg cgccgggccg tcttgcccaa
      421 cgcggtcctc ttccggcgca gcccctggtg gccgcccggc cgtatgcgcg agcggcagac
      481 aacacggaac cacccgccgg cggcaaccgc cagggacaac acacacgcga ggagatagag
      541 ggccgccggc aggctatggc gatggatggg ccaggcgccg gtaatccctg ccgggatggg
      601 caggaggaac gccggcaagg gtgcccagga tccgtatgca atcggcagga agtagctgat
      661 catcattacc tatacgcgcg gatcctaccc agcggtgcaa ggcatcccga ccccgataat
      721 acaaagggga ggaccgcggt ggtcctcccc ttgctcagca gtcgtggtgc cgcggattag
      781 cgggcgacgc gggtggcgcc ggcgtagtgg gcggtccagt aggggctcca cagattgctc
      841 acggtgacgc cgataccgta gtcggcggcg tggacgatct tgccgttccc ggcatagatg
      901 gcgatgtgcg agatgcccct gcgataggtg ttggcgaagt agaccaggtc accgggctgg
      961 agttggctgc tgctgatgtg cgggccggcg gcaatctggc tgctcatggc gcggcccagg
     1021 ccgatgccgg ccttattgaa cacatagtag acaaagccgc tgcagtcgaa gcccgacggg
     1081 ctggtgccgc cgtagcggta ggcatagccc acgtagttca tcgccacgct gaccacgccg
     1141 cccgcgctgc tgcgggccgc gggcggctgc gtggtgcccc gcgccgtacc ggtgcgcgcg
     1201 gtggtcgtgg tcgacttctt cgcggtggcc tgcgtcgtct ttttcggcgc gggggcgctc
     1261 accaggtacg tggcggacgc ccagccggtg acgttggcgt tggagacctg gtaccaggaa
     1321 tcgccgttct tgtccttggt cgggcctttc acgatgctga acaccgtgcc ctgaccggcg
     1381 gtggacttga tcgtgccgcc gggcgtgctg cgcagacgaa gcgccgcgcc accgggtgtg
     1441 gccaccttcg catacccctt cagcgtcgcc ggccccttgg ccgtagtcgt cttgctgctc
     1501 gtggtggcct tgctgccgct gctgctgctg ctgcccgccg ggccgaggta gcgcgtatcg
     1561 acccagccga tgcggccgtt gctgttgaca gtcacgcgcc accaactgtg tccggcgcca
     1621 tccttgaccg gaccctccag gacctgcacc ttatcgccct cgcccagggt gcccttcacg
     1681 tcgtagccga cgcccgcgcc ctcacggaaa cgcacgccgc cgccgccggt attcgtgacc
     1741 acggcgggct tattagggcc gaccccggct gccgccgcgg tgggcgcaaa cgacacgctc
     1801 gtcgccagta acgacgacac cgagaacaga gctgcgacta ccgcttggcg gatattaact
     1861 gctgagtaaa gtttcatacg aaaaagcctc atcctcctga aagattagct gtgcggcgac
     1921 cctaatgcgt ggtccgatcc gtttgccatt acaaccttga agccggatgg agcggggtcg
     1981 cgtcactccc gcgaaagaac aaccaatcgt tccgaacaac caccagagcg gtcaggcgat
     2041 cgataacggg gctggctgct tcccgatgag gagacacagc agatcaaggt acaccggggc
     2101 gcgccggttc aggacgcgca gcgtgcggcg gtgcggggag caccggaaca ggggattgta
     2161 gcgggggtgg gagctggtgc gaaaactgtg gacactggac gcgcattccg gcggagccat
     2221 ccgggtgcgc cgtcgcagtt tacgggagcg gaaagcgatc aagaaccaga ggactaaccg
     2281 caggcggtgg ggcacgcgca tagcttggtt gctgaaaact atttcagtgc ccaaagttcg
     2341 cccgctcctt cttgcctgag tactcggtct tttgtcgctc cggcgatagg tcttttgtcc
     2401 cttcgcctgt ggcagaatat catagcgccc agaccatgtc aagcgtagaa acgtcaataa
     2461 tatatcaata tcgggcatat ctcgcgttag aaatacgtcc cgagggacca ttgcgacggg
     2521 gtcggagacc aaaaaccggg tagtgctaat gtcccttttt ctactaataa acagcagatc
     2581 ccatattaga cggccccaag atcgccgcaa ccatgccgtg cctggatccc gatcacggct
     2641 gaaatcattt tgtgacccct gcaatgggga atctgatttc cgggactacc ggtccccagt
     2701 ggggggcgga tgcgccccgg ccctggtggg gccaattggg cagcgcatta atactcttgc
     2761 agccagcgcc attcttcgcg ggcgcgaatg agttgcagca gattgatgag atagtgggcg
     2821 accaggggcg ccagcaggct gcccgtggcg aggaacaaga cgctgagccc ggcgccgatg
     2881 ccggcggcga cgatcatgcc gagggtgccc tgcgggctgt gcatgatgcc gaagatgacg
     2941 ctgagcacgg cgatgaggat ggggggcggg atcagcacgc tgaagccgcc caacaacagc
     3001 ccgcggaaga gcacctcctc cagcaacacc gccagcagca tggccgcggg caccagcagc
     3061 cattcgcggc gggtgcgcgg aaagacgttc ttcatcacca cgggcgagta gacgcccttg
     3121 ccgaagcggc caatggccca gtgggtgatc aggttgacga cgatctgcgc ggcgaggccg
     3181 aaaatgatgc ccagggccgc gatttgcagc gggtgggtga aggtccagcc gaactggtcc
     3241 ggtggcaaac cgctgatggc ggcgagcccc acgcacacgg cgataaagcc ggccttaacg
     3301 gcgttctcga cgggggccag caacagattc tcctggacgg gcacgacgcg caacagcttg
     3361 ccgctctggt aggtcaccca ggccagcaag ccaagcagca gcaatgatcc gaggaggaag
     3421 atcacgaaca tagctgcaat tgtagcagcc gcccgtgatg agtgtcaagc accggcctaa
     3481 ccccccggcc cccttcccgg cgcgggaagg gggagaggac gagccaagcc accgagtcgt
     3541 ggggtttgac accgggcggc gggcgccggt acaatcgagc tacgatggat gagcgacggg
     3601 ggcggtgggc agcggtgggc gcgctggcgg gcgtcgccct ggtgctggcg gtggccgggg
     3661 cgctgctgcc gcagggcgtc ttctggcact ccgacgaggg ggccaagttc ctccagatgg
     3721 tcaacttgcg ctgggggccg gacggccttg acaccagcat ctactatccg ggccgggcgc
     3781 tcgatccgct gctccagttc gtgccgttcc accccaagca gtaccacagc gacgccgccg
     3841 ggcagatcta cctgcaatgg cccattttcc tggccctgtt cacgcggccc ctgtacgacc
     3901 tgctcggctg ggcggggctg tatgtgctgc cctgggccgg ggcgctggcc gcctgctggc
     3961 tgacctaccg gctggcccgc gccgtgggcg cgccgcccgg ctgggcctgg gtcgctatcc
     4021 cgctgctggg gctgaccacg ccgctaggct tctatagctg ggtcttcttc gagcacacgc
     4081 tggccgccgc gctggtggcc ggggcggcgc tgctgatcgt gcgcgggctg ccgcagcaag
     4141 attggcggcg cctggcgggc gcgggcgcgc tggtgggcgc aggcatctac ctgcgctccg
     4201 agttgtatat cctcgtcctg gccgcgggcg ggctggtcgc cgtgcgagca ttcgccgcgc
     4261 tgctgcccgg cgacgatcgg cgccccgcgc tcgaccgcgc cgccgcctgg cgtgtcgtgc
     4321 ggctgggggg cgcggggctg gtcgggctgc tgattgtgtt gctgccgctg tggggctact
     4381 acgccgccag cgcgggcggc ccgctgccgc agcacgccac gtg
//
LOCUS       DASUYC010000913         2470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1768626, whole genome shotgun sequence.
ACCESSION   DASUYC010000913 DASUYC010000000
VERSION     DASUYC010000913.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1768626"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /gene="cysC"
                     /locus_tag="VF276_14955"
     CDS             <1..611
                     /gene="cysC"
                     /locus_tag="VF276_14955"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEX6608201.1"
                     /translation="PAWFTRPAVAAILAEAYPPRTRQGVCVWFTGLPGAGKSTLAEVV
                     QARLLERGRRVTLLDGDVVRTHLSAGLGFSRADRDTHIRRVGWVAAEVVRHGGVAICA
                     AISPYAATRAEVRQMVGPDQFVEVYVSTPAPVCEARDPKGLYARARRGELSGFTGVDD
                     PYEPPAAAQVIVDTSHSDPQDDGGRIVAYLEAAGFLAPVAAL"
     gene            691..1146
                     /locus_tag="VF276_14960"
     CDS             691..1146
                     /locus_tag="VF276_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020474711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6608202.1"
                     /translation="MEAVIFIGLQATGKSSFYLARFFHSHIRINLDMLKTRRREDLLL
                     QACLAMQQSFVVDNTNPTLAERARYIPPARAAGFRIAGYYFESSLGDAIRRNSTRAPE
                     AQVPAKAIGATRKKLQLPTSGEGFDALYTVQIAPAGGFEVAPWDGEPAL"
     gene            1434..>2470
                     /locus_tag="VF276_14965"
     CDS             1434..>2470
                     /locus_tag="VF276_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX6608203.1"
                     /translation="MVADQRIWTYCAMCVSRCGAIAVVEDGRFVALEPDPSHPTGQAL
                     CAKGRAAPELVYHADRLLYPLKRTRPKGDPDPGWQRISWDEALDITAARLRTIAQTQG
                     PESVVFSVSSPSTSASDDSTVWIQRLMNAFGSPNLCAAMELCGWGRYQAPQYTFGASV
                     PGVYMPDLEQAGCILFWGYNPHVARIAHAVATTAALQRGARLIVVDPRRTGPANKADL
                     WLQVRPGTDAALALGIAHIMIERGWYDQDFIRAWTNGPLLVRMDNGRLLTERDLSDTG
                     SAQRYVAWDAVTAGPVLYDPATGAYAPETEVPALSGEFPIETGEGRVVCRPAFALMAE
                     LCRRYTPARVA"
BASE COUNT          373 a          863 c          821 g          413 t
ORIGIN      
        1 tgcccgcatg gttcacccgg cccgccgtcg cggccatcct ggccgaggcc tatcccccgc
       61 gcacccggca gggggtctgt gtgtggttca ccggcctgcc gggcgcgggc aaatcgacgc
      121 tggccgaggt cgtccaggcg cggctgctgg agcgaggccg ccgcgtgacg ctgctcgacg
      181 gcgacgtagt gcgcacgcac ctgagcgccg ggttgggctt cagccgcgcc gaccgcgata
      241 cccacatccg ccgcgtgggc tgggtcgccg ccgaagtggt gcgccacggc ggtgtcgcga
      301 tttgcgccgc tatcagcccc tacgccgcca cccgcgcgga ggtgcggcag atggtcggcc
      361 cggaccagtt cgtggaggtt tatgtcagca cccccgcgcc ggtctgtgag gcccgcgatc
      421 ccaaagggct ctatgcgcgc gcccgccgtg gcgagttatc cggcttcacc ggggtggacg
      481 acccctatga gccgcccgct gccgctcagg tgatcgtgga caccagccac agcgatcccc
      541 aggacgacgg cgggcggatc gtggcctacc tggaggccgc cggctttctg gcccccgtgg
      601 cggccctgtg agtcgtttct gttgggccgt gccatccgct atcatcccgg tagcggcgcc
      661 gggacccgta gcgccgcagg aggacacctg atggaagcag tgattttcat cggcttgcag
      721 gccacgggca agtcgtcgtt ctacctggcg cgcttcttcc atagccacat ccgcatcaac
      781 ctcgacatgc tgaaaacgcg ccggcgcgag gacttgctct tgcaggcctg tctcgccatg
      841 cagcagtcgt tcgtggtcga taataccaat cccacgctgg cggagcgggc ccgctacatc
      901 ccgccggctc gcgcggcggg ctttcggatc gccggctact acttcgagtc cagcctgggc
      961 gacgccatcc ggcgcaacag cacccgcgcg cccgaggccc aggtgccggc caaagccatc
     1021 ggggccaccc gcaaaaagct gcaactcccg acctccggcg aggggttcga cgccctgtat
     1081 accgttcaga tcgcccccgc gggcggcttc gaggtcgcgc catgggatgg ggaaccggca
     1141 ttatgagtga tcactttgca gcgcagatgg gcgccggagt cctttcctgg cctgcgggtg
     1201 ctggcgtggc tgcgccgtgg catctcgctt tcgggtcgag aatcccatat aatttgtgag
     1261 gaaccaggcg ccgtggccgg ctggttcacc aacgcaagcg cgccggttca cgccccgctc
     1321 ccgttccgcc acctctatgt agtagaccac gtacaacgaa ggaacgcatc accctcctgg
     1381 gcaggccccc accgcatcgc actcccaccg gttgacagta aggaggcatc acgatggtag
     1441 cggaccagcg aatttggact tactgcgcaa tgtgcgtctc ccgctgcggc gcgatcgccg
     1501 tcgtcgagga cggccgcttc gtcgcgctgg agccggatcc gtcccatcct acgggccagg
     1561 ccctctgcgc gaaggggcgg gcggcacccg aactcgtcta tcacgccgac cgcctgcttt
     1621 atcccctgaa acgaacccgg cccaagggcg atcccgatcc ggggtggcag cgcattagct
     1681 gggacgaggc gctagacatc acggcggccc ggctgcgcac gatcgcccaa acgcaagggc
     1741 cggagagcgt cgttttcagc gtgtcctccc catccacctc ggcctccgac gactccacgg
     1801 tctggattca gcgcctgatg aatgcgttcg gcagtcccaa cctgtgcgcg gccatggaac
     1861 tgtgcgggtg ggggcgatat caggccccgc agtatacctt cggcgccagt gttcccggcg
     1921 tctatatgcc cgacctggag caggcgggct gtatcctgtt ttggggctac aacccgcatg
     1981 tcgcccggat cgcccatgcc gtcgccacca ctgcggcgct ccaacgcggc gcgcggctga
     2041 tcgtggtcga tccccggcgc acggggccgg cgaacaaggc ggatctctgg ctgcaggtgc
     2101 gtccgggcac ggatgcggcg ctggcgctgg gcatcgccca tatcatgatc gagcggggct
     2161 ggtatgacca ggactttatc cgcgcctgga cgaacgggcc attgctcgtc cggatggaca
     2221 atgggcggct cctgaccgaa cgcgacctct cggacaccgg gagcgcgcag cggtatgtcg
     2281 cctgggacgc ggtgacagcg gggccggtac tctatgaccc ggccaccggc gcctatgcgc
     2341 ccgagaccga ggtgccggcg ctctccggcg aatttcccat tgagaccgga gagggccggg
     2401 tggtttgccg ccccgccttc gcgctcatgg ccgaactctg caggcgctat actccggcgc
     2461 gggtggcgga
//
LOCUS       DASUYC010000914         2345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1832238, whole genome shotgun sequence.
ACCESSION   DASUYC010000914 DASUYC010000000
VERSION     DASUYC010000914.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1832238"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VF276_14970"
     CDS             complement(<1..332)
                     /locus_tag="VF276_14970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608204.1"
                     /translation="MATIVEEPDRLEALAIWAGLMLLAVLPDWLSAIADPALMAAALT
                     SEVPEFAAGALVLRACRVDRVRLKDARWTALYDLTVAGPGADAGREVRLSGTLRPPGA
                     EEPPPASAP"
     gene            complement(564..2066)
                     /locus_tag="VF276_14975"
     CDS             complement(564..2066)
                     /locus_tag="VF276_14975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608205.1"
                     /translation="MSTVEEPQAAPAAAGAPATWRRFATLPDWLTAIDDPERVRRALL
                     QAVPEFASGALTLLACDVHRVRMKDARWTAQYRLTVAGPGDDSRREVTLRGTLVAPGA
                     PEPAPATGGAAFGTAGWQGYLPEVRLVLEPQPADAGLPALPHLTDPEEARALLEASIR
                     AASPAYRGLRLRAVTPRVMRYNPGSRCTILYRLDYPPELAAGHDWPDIVVAKTYHRDK
                     GRHAYAAMQALWESPLATGAVVTLAEPLGFLPEWNVLVQGPIREEQTLQDLIRTALHT
                     GAPDTQATLDGYLRQTAAGLAALHGSGVQARKRRTWEDERDEVQEIAGELESAVPELA
                     GATGPLLTRLEARAAAAPADPPGPAHGTFRPAQVLLNHGQIGFIDFDSFCEAEPALDV
                     ALFLEKIRDLGLSASAGDDDDDEEPLDPALYPARLAQVDRLCATFLDAYTAHRPISRE
                     RIALWETLYLLTLVLHCWTKVKPARLVHSMILLDHHLRAHHLAAGAPVAQ"
     gene            complement(2054..>2345)
                     /locus_tag="VF276_14980"
     CDS             complement(2054..>2345)
                     /locus_tag="VF276_14980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608206.1"
                     /translation="DLGQFLAYLRVAVQKAHRNAAVTPTALADDLGEVFYDAYVAAAD
                     TWLADEEQLRARTAVYEIVSLLRLALHSWQKLKPARIQNVLAVLEERMACLQ"
BASE COUNT          330 a          812 c          867 g          336 t
ORIGIN      
        1 ggtgccgaag ccgggggcgg ttcctccgca ccgggcgggc gcagggtgcc gctcagccgg
       61 acctcgcggc cggcgtccgc gccggggccg gccaccgtga ggtcatagag ggcggtccag
      121 cgggcgtctt tcaagcggac gcggtccacg cggcaggcgc gcaggaccag ggcgcccgcc
      181 gcgaactccg ggacctcgct cgtcagggcc gccgccatga gggccgggtc ggcaatcgcg
      241 ctcagccagt cgggcaagac cgccagcaac atcagccccg cccatatggc aagggcctcc
      301 agccgatccg gctcctccac aatcgtagcc atggccgcat gctccttata catcgtgccg
      361 gacatgccgt cgtagtcacc gcagagacgc agaggacgca gagatcatta aggataagtg
      421 aggttagagc ccgaaacggg cgcgtaagtt gccccaggcg aatcgcggtg tttaccggcg
      481 atgtcgatcc gtctaactct tgatcccctc tatcaaactc tctgcgtcct ctgcgcctct
      541 gcggtgagca tccggactaa attctattgg gccaccggcg cgcccgccgc cagatgatgg
      601 gcacgcaggt ggtggtccag caggatcatg ctgtgcacca gccgggccgg tttcaccttg
      661 gtccagcagt ggagcaccag ggtcagcagg tagagcgttt cccacagggc gatgcgctcg
      721 cgggagatgg gccggtgggc cgtgtaggcg tcgaggaacg tggcgcagag gcgatccacc
      781 tgggccaggc gggcgggata cagggcgggg tcgagcggtt cctcgtcgtc gtcgtcgtcg
      841 cccgcggagg cgctcagccc caggtcgcgg atcttctcca ggaacagggc cacgtcgagc
      901 gccggctcgg cttcgcagaa gctgtcgaag tcgatgaagc cgatctgccc gtgattgagc
      961 agcacctggg ccgggcggaa ggtgccgtgg gccggccctg gcggatcggc gggggccgcg
     1021 gcggcgcgcg cttccaggcg ggtcagcagc ggcccggtgg cgccggccag ttcgggcacc
     1081 gcgctctcca gttcgccggc gatctcctgc acctcgtcgc gctcgtcctc ccaggtgcgg
     1141 cgcttgcggg cctggacgcc cgatccatgc agcgcggcca gcccggcggc ggtctggcgc
     1201 aggtagccgt cgagggtggc ctgcgtgtcc ggggcgccgg tgtgcagcgc cgtgcggatc
     1261 aggtcttgca gcgtctgctc ctcgcggatc ggcccctgca cgagcacgtt ccactccggc
     1321 aggaagccca gcggctcggc cagcgtgacc acggcgccgg tcgccagcgg cgactcccag
     1381 agcgcctgca tggcggcgta ggcgtggcgg cccttgtcac ggtggtaggt cttggcgacg
     1441 acgatatcag gccagtcgtg gccggcggca agctccggcg ggtagtcgag ccggtagagg
     1501 atggtgcagc ggctgccggg gttatagcgc atgacgcgcg gcgtcacggc ccgcagccgc
     1561 aggccgcggt aggcggggct ggcggcgcgg atgctggcct ccaacagcgc gcgcgcttcc
     1621 tccgggtcgg tcaggtgggg cagcgcgggc aacccggcgt cggcgggctg cggctccagc
     1681 accagccgca cctcgggcag atagccctgc caccccgcgg tgccgaaagc cgcgccgccg
     1741 gtcgccgggg ccggctccgg cgcaccgggc gcgaccagcg tgccgcgcag cgtcacctcg
     1801 cggcgggaat catcgcccgg cccggccacc gtcagccggt actgcgcggt ccagcgcgcg
     1861 tccttcatgc gcacgcgatg cacgtcgcag gcgagcaggg tcagcgcgcc ggacgcgaac
     1921 tcgggcacgg cttgcagcag cgcgcgccgc acccgctccg ggtcgtcgat agcggtcagc
     1981 caatcgggca gcgtggcgaa gcgccgccag gtagcgggcg cgcccgccgc cgccggggcg
     2041 gcctggggtt cttctactgt agacatgcca tgcgctcctc tagcacggcc aggacattct
     2101 gaatccgcgc cggcttgagt ttctgccagc tatgcagggc caggcgcagc aggctgacga
     2161 tctcgtacac cgcggtccgg gcgcgcaact gttcctcgtc ggccagccag gtgtccgccg
     2221 ccgcgacgta ggcgtcgtag aagacctcgc ccaggtcgtc ggccagcgcg gtcggcgtga
     2281 cggcggcgtt gcggtgcgcc ttctgcacgg ccacgcgcag gtaggccagg aactggccca
     2341 gatcg
//
LOCUS       DASUYC010000915         3170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_696563,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000915 DASUYC010000000
VERSION     DASUYC010000915.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696563"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(414..1310)
                     /locus_tag="VF276_14985"
     CDS             complement(414..1310)
                     /locus_tag="VF276_14985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX6608207.1"
                     /translation="MNRAISRTPVGGWLARVLIVTGLCCLALTGLWAASAAAQSSTTL
                     SGYVFGYDGASLADATITVFHMPEHVAIGPPVHSNADGAWSIAAGNGPFVARAEHPGY
                     GTSEFTVYPSEYTTGITFLLPVSPTTESPPLVARVTGQVRDLDGVPLGGMAIYATNAL
                     ATGLKQVKGPPIFSATRTDADGRYTLDLPAGEIWLGIKTGAGWGYQQKPRQVQAGDNF
                     SDGDFVVAIRTGAQYFPPTATPAAAPTSTPTGNVGPIISPPPGMPVTGQPADSAWPAL
                     LLVGLLALLAGGILRGRPTVRR"
     gene            complement(1323..2345)
                     /locus_tag="VF276_14990"
     CDS             complement(1323..2345)
                     /locus_tag="VF276_14990"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX6608208.1"
                     /translation="MQALVWNGPHQMEVEEIACPQPGPGEVLIEVKAVGICGSDVHGY
                     TGASGRRAPGMVMGHEFAGVVRELGPDVTAPAAGTRVAVNPLIYCGTCPDCRAGHEEL
                     CRHRKSIGVNMGTHGGFAEYVTAPAINAIPLAGNVSFAAGSMAEPLAVGLRATNVADP
                     QRGQPIAILGGGTIGLCCLLACRQRGADPVFVTDVAPHKLEMIGRLGGRPQDSRQTDL
                     AALGREATDGRGFPVIIDAVAVSATIKQALPALAPGGTLMLVGLATPLVEVGLYDLVP
                     QERRITSAYAYTAAEYREAVDWLNRGTVDVAPLIEARCPLAEAPEMFARMGRGEVEAV
                     KVVVEV"
     gene            complement(2504..>3170)
                     /locus_tag="VF276_14995"
     CDS             complement(2504..>3170)
                     /locus_tag="VF276_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2088 domain-containing protein"
                     /protein_id="HEX6608209.1"
                     /translation="LPPAAFGGAEETRLLSDARRWLPGLPFDDLDVLVIDQIGKNFSG
                     AGMDTTVIGRILVPGQEENPRPRITAIVALDVSPESHGNAAGLGMADVTTRRLVGKMD
                     FHAFYLNGITSGTFGLRRSSIPFVMQDDYTAIALALHAAASAHPDQARLVRIPNTLDV
                     GALLIAESLCAEAEAAGCTVGPPLGPLTFDPTGQITPFPAALSHQPSAVSRQVTSSWD
                     RVG"
BASE COUNT          433 a         1099 c         1165 g          473 t
ORIGIN      
        1 tgggcgtcgg cgggagcggg gtacgagtgg gcagccagct accggccggg gtcggggcgg
       61 gcggggtgcg ggccaggggc gtgggcacgg gcaccgccgg cccggcgggg tccgtcccgc
      121 agccgctcag cagtagcacc agcaccagca gcgcggcgcg cgcgccgggg gatacgcggg
      181 gtggggcggt tcggcatcgg ctcctcctgg gtggatcggg gtggccgccg cgccagctat
      241 gcgcccccct gggtgcccgg attgtagcac atcccccggc ccgccgcaaa cgcccggcac
      301 tccacttgcc tctgttataa ggggagttct gccgctgccc cccgcactcc caccggcgcc
      361 ccccttcccg cggcgggaag gggggttggg gggttaggtt gggcgggcgg ggattagcgc
      421 cgaaccgtcg ggcgcccgcg caggatgccg cccgccagca gcgctagcag gcccacaagc
      481 agcagcgccg gccaggcgct gtccgccggc tggccggtca cgggcatgcc gggcggcggg
      541 ctgatgatcg ggccgacgtt gccggtcggg gtactggtcg gggcggcggc cggcgtggcg
      601 gtgggcggga agtattgcgc cccggtgcgg atggcgacga cgaagtcgcc gtcggagaag
      661 ttgtcgcccg cctgcacctg gcggggcttt tgctggtagc cccagcccgc gcccgtcttg
      721 atgcccagcc agatctcgcc ggcaggcaga tccagggtat agcggccgtc ggcgtcggtg
      781 cgcgtggcgc tgaagatggg cggccccttg acctgcttga ggcccgtcgc cagcgcgttg
      841 gtcgcgtaaa tcgccatccc gcccagcggc acgccgtcca ggtcgcgcac ctgcccggtg
      901 acgcgcgcca ccagcggcgg gctttccgtg gtcgggctta ccggcagcag gaaggtgatc
      961 cccgtcgtgt attcgctggg gtagacggtg aactccgacg tgccgtaacc ggggtgttcg
     1021 gcccgcgcga cgaaggggcc gttgcccgcg gcgatcgacc acgcgccgtc ggcgttgctg
     1081 tggaccggcg ggccgatggc gacatgctcc ggcatgtgaa acacagtgat ggtggcatcg
     1141 gccaggctgg cgccgtcgta gccgaagacg tagcccgata gcgtggtgct gctctgcgcg
     1201 gcggcggacg cggcccacag cccggtcagg gccaggcagc agaggccggt cacgatcagg
     1261 acgcgggcta gccacccgcc cacaggtgta cgcgaaatcg ccctattcat ccgaactcct
     1321 tcctatactt cgacgacgac tttcaccgcc tcgacttcgc cgcggcccat acgcgcgaac
     1381 atctccgggg cctcggccag ggggcagcgc gcctcgatca gcggcgccac gtccaccgtg
     1441 ccccggttca gccagtccac ggcttcgcgg tactcggcgg cggtgtaggc gtaggcgctg
     1501 gtgatgcggc gctcctgcgg caccaggtcg tagaggccga cctcgaccag cggcgtcgcc
     1561 aggccgacca gcatcagcgt gccgccgggg gccagcgcgg gcagcgcctg cttgatggtg
     1621 gcgctcacgg ccacggcgtc gatgatgacc gggaagccgc gcccgtcggt ggcctcgcgg
     1681 cccagcgcgg ccaggtcggt ctggcgcgag tcctgcggcc ggccgcccag gcgcccgatc
     1741 atctccagct tgtgcggggc cacatcggtg acgaacaccg ggtccgcgcc gcgctggcgg
     1801 caggccagca ggcagcagag gccgatggtg ccgccgccca ggatggcgat gggttgcccg
     1861 cgctgcggat cggccacgtt ggtggcgcgc agccccacgg ctagcggctc ggccatcgag
     1921 cccgcggcga agctgacgtt gccggccagc gggatggcgt tgatcgcggg cgcggtgacg
     1981 tactcggcaa acccgccgtg cgtgcccatg ttcacgccga tggacttgcg gtggcggcac
     2041 aattcctcgt gcccggcgcg gcaatcgggg caggtgccgc agtagatcag cgggttcacc
     2101 gccacgcgcg tgcccgccgc cggtgcggtc acatccggcc ccagttcgcg cacgaccccg
     2161 gcgaattcgt ggcccatgac catgcccggc gcgcgccgcc ccgacgcccc ggtgtagccg
     2221 tgcacgtcgc tgccgcagat gcccacagcc ttgacttcga tcaacacctc gcccggtccc
     2281 ggctgcgggc aggcgatttc ctcgacctcc atctggtggg ggccgttcca cacgagtgct
     2341 tgcatgtcgt ctatccttta cgaagccgtc agcggtcagc cgtcagcggt cagccgtcag
     2401 cggtcagcta tcaggtggtt atgaatgtat actggcgcca attcacgcac ttttagacga
     2461 attaagactc gctactcgtc acctgacggc tgaccactga cggctagcct actctgtccc
     2521 aactactcgt cacctgacgg ctgaccgctg acggctgatg gctgagagcc gccggaaacg
     2581 gcgtaatctg ccccgtcggg tcgaacgtga gcgggccgag cggggggccg accgtgcagc
     2641 ccgcggcttc ggcttccgcg cagagcgatt ccgcgatcag cagcgcgccc acgtcgagcg
     2701 tattcgggat gcgcaccagg cgtgcctgat cgggatgggc gctggccgcc gcgtgcaggg
     2761 ccagcgcgat ggccgtgtag tcgtcttgca tcacgaacgg gatggacgag cggcgcaggc
     2821 cgaacgtgcc cgacgtgatg ccgttcaggt agaaggcgtg gaagtccatc ttgccgacca
     2881 ggcggcgcgt cgtcacgtcg gccatgccca ggcccgccgc gttgccgtgc gactccgggc
     2941 tcacgtcgag cgcgacgatg gccgtgatgc gcgggcgggg gttctcctcc tgccccggca
     3001 ccaggatgcg cccgatgacg gtggtgtcca tgcccgcgcc gctgaagttc ttgccgatct
     3061 gatcgatcac cagcacgtcg aggtcgtcga agggcaggcc gggcagccag cggcgggcgt
     3121 cgctcagcag gcgggtttcc tccgcgccgc cgaaggccgc cgggggcagc
//
LOCUS       DASUYC010000916         2284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_847426,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000916 DASUYC010000000
VERSION     DASUYC010000916.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847426"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1742)
                     /locus_tag="VF276_15000"
     CDS             complement(<1..1742)
                     /locus_tag="VF276_15000"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX6608210.1"
                     /translation="MIRWIVGSSMQFRLIVVAAALAMVVFGVTQLPNMAVDVLPEFAP
                     PTVEVRTEALGLSADEVEQFITVPMEQDLLNGVAWLADIQSDSVPGLSSIVLTFEPGT
                     DIIRARQMVAERLTQAAVGLPHVSKPPTMLQPLSSTSRVMMIGLSSKTVSLIDMSVLA
                     RWTISPRLMGVPGVANVSTWGQRDRELQVLVDPKRLHDKGVSLLQVLETTGNAMWVSS
                     LSFVEASTPGTGGFIDTSNQRLGIRHIFPISSPDGLAQVPVEGAPSLRLGDVANVVED
                     HQPLIGDAVTNNSTSLLLVVEKFPGANTLDVTRGLEEALTALQPGLPGIQIDSTIYRP
                     AGFIETALSNVTLLLIIAGVLLVLALGAFLFEWRTALISLIAIPLSLLAGGVVLYLRG
                     ATLNTMVLAGFVIAVGVVVDDAIIDVENIRRRLRAARQAGSDKSTASIILEASLEVRN
                     ASVVATLILLLALVPIFFIGGLAGPFFQALATSYALAVLASLVVALLVTPALCVLLLG
                     PAPLERRESPLARGLQRGYERMLKRLLGMPRMALATAALLIVLGLLVVPFLGQSLFPT
                     FKEGDLLIHLDATA"
BASE COUNT          460 a          756 c          730 g          338 t
ORIGIN      
        1 ctggccgtgg catcaagatg gatcaacagg tccccctcct tgaaggtagg gaacagggac
       61 tggcccagga agggtacgac cagcagcccg agcacgatca gcagcgccgc cgtggcaagc
      121 gccatccgcg gcatgccaag cagccgtttc agcatccgct cgtagccccg ctgcaacccg
      181 cgcgccagcg gcgactcgcg ccgctccagc ggcgccggcc ccagcagcag cacgcacagc
      241 gccggtgtga ccagcagcgc caccaccagc gaggccagca ctgccagcgc atacgaggtc
      301 gccagcgcct ggaagaaggg accggccagc ccgccgatga agaaaatcgg caccaacgcc
      361 agcagaagga tcagtgtcgc gacgacgctg gcgttgcgca cctccagcga ggcttccagg
      421 atgatgctcg ccgtcgattt gtcgctgccg gcctgtcggg ccgcccgcag ccgccgcctg
      481 atgttctcca cgtcgatgat cgcgtcgtcg accaccacgc ccacggcaat cacgaacccg
      541 gccagcacca tggtgttgag ggttgcgccg cgcaggtaga gcaccacccc gcccgcgagg
      601 agcgacagcg ggatggcgat caggctgatc agcgccgtcc gccactcgaa cagaaatgcg
      661 cccagggcca gcaccaggag cacaccggcg atgatcagca gcagcgtcac attgctcagc
      721 gcggtctcga tgaagccggc gggccggtag atcgtggagt cgatctggat accgggcaga
      781 cccggctgca acgccgtgag cgcctcttcg aggccacgcg tcacgtccag ggtgttagcg
      841 ccggggaact tctcgaccac cagcagcagg ctggtgctat tattggtgac ggcgtcgccg
      901 atgagcggct ggtggtcctc caccacgttc gccacgtcgc cgaggcggag tgatggagcg
      961 ccctcgacag gtacctgggc cagcccgtcc ggtgaggaga tggggaaaat gtggcggatg
     1021 ccgagccgct ggttggacgt gtcgatgaac ccgccggtgc cgggggtgga cgcctcgaca
     1081 aagctcagcg acgacaccca catggcgttg ccggtggttt ccagtacctg cagcaacgac
     1141 acgcccttat cgtgcagccg tttcggatcg accagcacct gcaactcgcg gtcccgctgg
     1201 ccccaggtgg acacattcgc cacacccggc acgcccatca agcggggcga gatcgtccag
     1261 cgcgccagca ccgacatgtc gatcagcgac accgtcttcg aggacagccc gatcatcatg
     1321 acccggctgg tcgacgacag cggctgcaac atggtcggcg gcttggacac gtggggcaac
     1381 ccgaccgcgg cctgggtcag gcgctcggcc accatctgcc gggcccggat gatgtccgtg
     1441 ccgggctcga aggtgaggac gatcgacgac aggcccggca cggaatccga ctgaatatcg
     1501 gccagccagg ccacgccgtt gagcaggtcc tgctccatcg gcacggtgat gaactgttcc
     1561 acttcgtcgg cggacaggcc gagtgcttcg gtccgcacct ccaccgtcgg cggggcgaac
     1621 tcgggcagga catccaccgc catattcggc agttgggtga cgccgaatac caccatcgcc
     1681 agggcggcgg ccacgacgat gagccggaac tgcatgctcg acccaacaat ccaacgtatc
     1741 atagctggag acctccttat gcagtcatgt tccccgtgcg cagggaatac tgaagaaccg
     1801 ccaacatgac ccggagatgc ttaaaagcct gcccgccagg tgctggaccc aggagatggg
     1861 aaatggtgct gtgccgacca atcacggagg tgtgccagcc gtaccctggc agatctgcgg
     1921 gtgaatgcgc gggcgtcaca cgcgccgcca cgctacggag cggccccggt ccgtccttga
     1981 gcgccggcga tggacttcac gccattcgat acccacatcg tacgctatcc atccccctgg
     2041 gcgctatcag ggaaaaccct gatatctatg atagggattg cctgaaaaag tacgagagaa
     2101 tgcctgaaat gtaggtcggc ttccggtgcc gaccggagct aggaagcctg ggccaaccgg
     2161 cgagaaatct gcgatctgcg gcgagtcggg tgcacaaccc agccacacgg gggatggtag
     2221 aggggcagcg ccggaggttc gactttaccg gcacaaactc gtttgtcaac aaatattgac
     2281 taag
//
LOCUS       DASUYC010000917         3859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_2001520, whole genome shotgun sequence.
ACCESSION   DASUYC010000917 DASUYC010000000
VERSION     DASUYC010000917.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2001520"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /gene="rseP"
                     /locus_tag="VF276_15005"
     CDS             complement(<1..832)
                     /gene="rseP"
                     /locus_tag="VF276_15005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEX6608211.1"
                     /translation="MSWLWIVPVLGILVVIHELGHFVSARMLGIRVEEFGIGFPPRLF
                     AIRHNNIDYSLNWLPIGGFVRILGENGDSDAPDSFGRAAAWKRIIVLAAGSFMNLLLA
                     LVLFIGLGLFGEPVPDSTQVGFAAIVPNSPASTAQLQPGDLLLAVDGTPVSDINAARD
                     ILNNNRGRQIDLTVSRNGQEMQVPVTLRQGPEPALGVSLEAAVSPIRVADVQPGTPAA
                     TAGLQAGDIIKTVNGQEASSTLTFAQAMDRVKSGTTTITVERNGAPVTLTAEVANSGL
                     E"
     gene            complement(829..2049)
                     /gene="dxr"
                     /locus_tag="VF276_15010"
     CDS             complement(829..2049)
                     /gene="dxr"
                     /locus_tag="VF276_15010"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013062274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX6608212.1"
                     /translation="MLDSTAHPDPAQPVGIALLGCTGSIGTQTLDVLRGLGPGYRVVT
                     LAAGRDAAGLLAAATALPQPPQLLALGDAAAPAATDAQGRPIPRGEAALVDCATHPDV
                     DLVVVATSGQAGFAPTLAAIAAGKQIALANKEVLVMAGELVTAAARRAGVLLRPIDSE
                     HSAIWQSLQGETVRDLNTYPEIERIIITASGGPFRTWSAERLLDVTPAMALKHPNWDM
                     GAKITIDSATLANKGLEVIEAHWLFDLPFEQIDVLVHPQSIIHSMVEFRDGAIKAQLG
                     VPDMRVPIQYALTWPLRAASTFPRIDWHSVRELDFELPDVERFPCLRLAWEAGKRGGT
                     FPTVLAAADEVAVLGFLQERWRFVDIPRIIEQTLADHESAATPHPDLDAIKAADAWAR
                     RHVLEVAASRRSNA"
     gene            complement(2129..2899)
                     /locus_tag="VF276_15015"
     CDS             complement(2129..2899)
                     /locus_tag="VF276_15015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608213.1"
                     /translation="MTAPVSRPPLGRRLAAGDVQVWQIGLLALLIVVVLLPESLRAVV
                     LGLWGGAALLFRMAGDGPLPASSLSRYAVPLLAYAILVAPGLAGQIAWTGAALAPVTL
                     LLLAGLLPRPDTAPAARDWAAQVVGVLWAALGVLALAGLATVPELLGSAAVVVLAGLL
                     AYTAGRRAGLIQGLSERVAIGMGATLALSAPIMLSTGLPAVGMVLGLVGGLVLANLAP
                     PPPVVARPYLSAVIAVQHQLLFVAPLTVVLLWLVRTFL"
     gene            complement(2931..3656)
                     /locus_tag="VF276_15020"
     CDS             complement(2931..3656)
                     /locus_tag="VF276_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEX6608214.1"
                     /translation="MSDERPRISPVPRHVAMIMDGNGRWAKQRGLPRLAGHRAGTENI
                     RNIVQAAVEQGVGYLTLYAFSTENWSRPDSEVQGLLRILAEVIERETPELDRNGVQLR
                     HLGSMEGVPQDLADAINGALDFTRHNNRLILSVAFNYGGRAEIVEAVRRIVRAGISPD
                     AIDEETISRHLYTADIPDPDLIIRTAGEQRLSNYLIWQAAYAEYWFTPAYWPDFDKDV
                     FYQALLDYSRRKRKFGMTEDQVS"
     gene            complement(3661..>3859)
                     /locus_tag="VF276_15025"
     CDS             complement(3661..>3859)
                     /locus_tag="VF276_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome-recycling factor"
                     /protein_id="HEX6608215.1"
                     /translation="VEDCRVAIRNIRRHALNDLKELEDEKLIAEDEHKRATEKLQTLV
                     DRYIRDAEHVGDAKEAEVMEV"
BASE COUNT          651 a         1303 c         1314 g          591 t
ORIGIN      
        1 cctccaggcc gctgttggcg acctccgcgg tcagcgtcac cggcgcaccg ttgcgttcca
       61 cggtgatggt ggtggtgccg ctcttgacgc gatccatggc ctgggcgaag gtgagcgtgc
      121 tactcgcctc ctggccgttc accgtcttga tgatgtcgcc ggcctggaga cccgccgttg
      181 ccgcgggcgt gccgggttgg acatcggcga cgcggatcgg cgagacggcg gcttcgaggc
      241 tgacaccgag cgcgggttcg ggcccctggc gcagggtcac gggcacctgc atttcctggc
      301 cgttgcggct gaccgtcagg tcgatctggc ggccccggtt gttattcagg atatcgcgcg
      361 ccgcgttgat gtcgctcacc ggcgtgccgt ccacggccag cagcaggtcg cccggctgca
      421 attgcgcggt cgacgcgggc gaattcggca cgatggcggc aaagccgacc tgcgtgctgt
      481 cgggcaccgg ctcgccgaac aggcccaggc cgataaacag caccagcgcc agcagcaggt
      541 tcatgaacga cccggcggcc agcacgatga tccgtttcca ggccgcggcc cgcccgaagc
      601 tgtcgggggc atcggaatcg ccgttctcgc cgaggatgcg cacgaagccg ccgatgggca
      661 gccagttgag cgaataatcg atgttattgt ggcggatggc gaacaggcgc gggggaaagc
      721 cgatgccgaa ctcttcgacg cggataccca gcatccgtgc cgacacaaag tgtcccagtt
      781 cgtggatgac gacgagaata ccgaggaccg ggacgatcca taaccaactc atgcattact
      841 ccttcgggac gcggccacct ccaggacgtg gcgccgcgcc caagcatccg cggctttgat
      901 agcatcgaga tcgggatgcg gcgtggcggc ggattcgtgg tccgccagag tttgttcaat
      961 tatacgcggt atatcgacaa accgccagcg ctcctgcaaa aagccgagca cggctacttc
     1021 gtcggcggcg gccaggaccg tggggaaggt gccgccccgc ttgcccgcct cccaggccag
     1081 gcgcaaacag gggaaccgct ccacgtccgg cagctcaaag tcgagttcgc ggacgctgtg
     1141 ccagtcgatg cgcggaaagg tactcgccgc ccgcaagggc caggtcagcg cgtactgaat
     1201 gggcacccgc atgtcgggca cgcccagttg cgccttgatc gcgccatccc ggaactcgac
     1261 catggagtga atgatcgact gcggatgcac cagcacgtcg atctgctcga acggcaggtc
     1321 gaacagccag tgcgcctcga tcacctccag gcccttgttc gccagggtgg ccgagtcgat
     1381 ggtaatcttg gcgcccatgt cccagttggg gtgcttgagc gccatcgcgg gcgtcacatc
     1441 gagcaaccgc tcggcggacc aggtgcgaaa cggcccgccc gacgcggtga tgatgatccg
     1501 ctctatctca ggatacgtgt ttaaatcgcg aacagtttcg ccctgcaacg actgccagat
     1561 ggcgctatgc tcgctgtcga tggggcgcag cagcacgccc gcccggcgcg cggcggccgt
     1621 gaccagctca ccggccatga ccagcacttc cttgttcgcc agggctatct gcttgcccgc
     1681 ggcaatggcg gccagggtgg gcgcgaagcc cgcctgcccg ctggtcgcca cgaccacgag
     1741 atcgacatcg gggtgggtgg cgcaatcgac cagggcagct tccccgcgcg gaatcggacg
     1801 gccctgggca tcggttgcgg cgggcgcggc ggcatcgccc agggccagga gttgcggcgg
     1861 ctgcggcagc gccgtggcag cagccagcag gcccgcggcg tcgcgcccgg cggccagggt
     1921 cacgacccgg tagcccggtc ccagcccgcg caggacatcc agcgtctgcg tgccgatgga
     1981 gccggtgcag cccaacaggg cgatgccgac cggttgggcg gggtcaggat gagcagttga
     2041 atcgagcata atggtccaca cagattccta gttcaaggga gaggctatct cccggtgcgt
     2101 acgccgcagg aaagagaatc gcccgcttct ataaaaacgt ccgcaccagc cacaataaca
     2161 caaccgtaag gggcgccacg aagaggagtt ggtgctgaac cgcgatcacg gcgctcaaat
     2221 agggccgggc aacgaccggc ggcgggggcg ccaggttcgc caggacgagg ccgccgacca
     2281 gccccaggac catgcccacc gccgggaggc cggtcgagag cataatcggg gcgctgaggg
     2341 ccagggtggc gcccatgccg atggcgaccc gctcgctcaa gccctggatc agcccggcgc
     2401 gacgcccggc agtgtaggcc agcagcccgg ccagcaccac caccgccgcc gaacccagca
     2461 gttcgggcac ggtggcgagc ccggccagcg ccaggacgcc gagcgccgcc cagagcacgc
     2521 ccaccacctg cgcggcccaa tcccgcgcgg cgggcgcggt gtcggggcgc ggcagcagcc
     2581 cggccagcag caagagcgtg accggggcga gcgccgcgcc ggtccaggcg atctgcccgg
     2641 ccaggcccgg cgcgaccagg atcgcgtagg cgagcagggg cacggcatag cgcgataagc
     2701 tactcgccgg cagcggccca tcgcccgcca tccggaacag cagtgcagcc ccgccccaca
     2761 gccccagcac gaccgcgcgc aggctctccg gcagcagcac caccacgatc agcagcgcca
     2821 gcaagccaat ctgccagacc tgcacatccc cggcggccag gcggcggccc agcgggggcc
     2881 gggacaccgg ggcggtcatg cgcgcgacct cccgtggatc acgctcaagc tcagctaacc
     2941 tggtcttccg tcatgccgaa tttgcgcttg cgccggctgt agtccagcag cgcctggtag
     3001 aagacatctt tatcgaagtc gggccagtag gccggggtaa accagtattc ggcgtaggcc
     3061 gcctgccaga tcaggtaatt gctcaggcgc tgctccccgg cggtgcggat gatcagatcg
     3121 ggatcgggga tgtcggcggt gtagagatgc cggctgatcg tctcctcgtc gatggcgtcg
     3181 ggcgagatgc ctgcgcggac gatgcggcgc accgcctcga cgatctcggc ccggcccccg
     3241 tagttgaagg ccacgctgag gatcaggcgg ttgttgtggc gggtgaagtc gagcgccccg
     3301 ttgatggcgt cggccaggtc ctgcggcacg ccctccatgc tgcccaggtg gcgcagttgg
     3361 acgccgttgc ggtccagctc cggcgtctcg cgctcgatca cctcggccag gatgcgcaac
     3421 agcccctgga cctcggagtc cgggcggctc cagttctcgg tcgagaaggc gtagagggtc
     3481 agatagccca cgccctgctc gaccgcggcc tggacgatat tgcggatatt ctccgtgccg
     3541 gcgcgatgcc cggccagccg cggcaggccg cgctgcttgg cccagcgccc attgccgtcc
     3601 atgatcatgg cgacatggcg gggcacggga cttatccggg ggcgctcgtc gctcatcccc
     3661 ctagacctcc atgacttcgg cttctttggc gtcgcctaca tgctcggcgt cacgaatata
     3721 acggtcgacg agggtctgta atttctccgt agcgcgcttg tgttcgtcct cggcgatcag
     3781 tttctcgtct tccagttcct tgaggtcgtt cagggcatgg cggcggatat tgcggatcgc
     3841 cacgcggcag tcctcgacg
//
LOCUS       DASUYC010000918         2774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1769884, whole genome shotgun sequence.
ACCESSION   DASUYC010000918 DASUYC010000000
VERSION     DASUYC010000918.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1769884"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1286)
                     /gene="hutU"
                     /locus_tag="VF276_15030"
     CDS             complement(<1..1286)
                     /gene="hutU"
                     /locus_tag="VF276_15030"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019913161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEX6608216.1"
                     /translation="MTSTVGRANAVPAQDAQVAPRPMRAPRGATLNCKGWLQEAAYRM
                     LQNNLDPEVAERPDELVVYGGRGRAARSWPALLKILEMLRTLEDDETLLIQSGKPVGV
                     FRTHADAPRVLLANSNIVPAWATQENFDRWERDGLTMYGQMTAGSWIYIGTQGILQGT
                     YETFGALARAQGWGSLKGKLVVTAGLGEMGGAQPLAITMNEGVGLLIEVDRWRAERRL
                     ALRQLDTISDNLEEAMTWAEAARARGEPMSIGLIGNAAVLLPELLERGVVPDVVTDQT
                     AAHDVLYGYIPAGLSLGEAADLRARDPDEYRRRALDSMAEHVRTLLAWQAKGSVVFDY
                     GNNLRQRAFDAGVTNAFDYPGFVPAYIRPLFCEGKGPFRWVALSGEPEDIYATDRAVM
                     ELFPEDEHLHRWLRMAQEQVAFQGLPARICWLGYGER"
     gene            complement(1283..>2774)
                     /gene="hutH"
                     /locus_tag="VF276_15035"
     CDS             complement(1283..>2774)
                     /gene="hutH"
                     /locus_tag="VF276_15035"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEX6608217.1"
                     /translation="VDSRQVVYGITTGFGAFKDRVIPLPDLARLQANLITSQTVGVGP
                     ALSREETRALMIARAQTLALGYSGLRRGTLQLLIDMINRGVHPVIPAQGSVGASGDLA
                     PLSHMAHAMMGGGLAEYQGTLQPACDALRAAGLAPVELIAKEGLALCNGTSQISGLLA
                     LATADAANLALAADICGALSFEALQGIPAALDPRIHAARGQGGQIAAAAHLRALLAGS
                     EFVWQDHVALRAPDAAGSGGPDPAAHKVQDAYSLRCMPQVHGPASDALAYVRGIVETE
                     LNAAVDNPLIFGAGAVGMDDEVAVLSGGNFHGMPVAVAADFLGIPFCGVASISERRQA
                     RLTDVHENGGILPPFLITNGGLNSGFMMVQYSAAALVSENKSLAHPASVDSIPTSANQ
                     EDHVSMGPIAARHARAIMRNTTLVLAMELLTAGQAIDLRREAAGRPLRLGRGTQVAYD
                     LLRERVAFRTEDAPFTADIAWADELISSGRLVREVEAAIANEGGSP"
BASE COUNT          409 a          984 c          958 g          423 t
ORIGIN      
        1 cgctcgccat agccgagcca gcagatgcgc gctggcagac cctgaaacgc gacctgctcc
       61 tgcgccatgc gcagccagcg gtgcagatgc tcatcctcgg gaaaaagctc catgacagcc
      121 cggtcggtag cgtaaatatc ctccggctcg ccggaaagcg ccacccagcg gaacggcccc
      181 ttgccttcac agaacagcgg gcggatgtag gctggcacga agccgggata atcaaaggcg
      241 ttggtcacgc ccgcatcaaa ggcgcgctgg cgcagattgt tgccgtaatc gaacacgacg
      301 ctgcctttgg cctgccatgc cagcagcgtg cggacatgct cggccatcga gtcaagcgcc
      361 cggcggcggt actcgtcggg gtcgcgggcg cgcaggtcgg cggcctcgcc gagcgacagg
      421 ccagccggaa tgtagccgta cagcacatca tgggcggcgg tctggtcggt gacgacatcc
      481 ggcacaacgc cgcgctccag cagttcgggc aacagcaccg ccgcattgcc gatcagcccg
      541 attgacatcg gctcgccgcg cgcccgcgcc gcttcggccc atgtcatggc ttcttccagg
      601 ttgtcgctga tcgtgtcaag ctgccgcaac gccagccgcc gctcggcccg ccagcggtcg
      661 acctcaatca gcagcccgac gccttcgttc atggtgattg ccagcggctg cgcgccgccc
      721 atctcgccca ggccagccgt caccaccagc ttgcccttca gactgcccca gccctgcgcc
      781 cgcgcgagcg cgccgaaggt ttcataagtg ccttgcagaa tgccctgcgt gccgatataa
      841 atccagcttc cggccgtcat ctggccgtac atcgtcaggc cgtcacgctc ccaacggtcg
      901 aagttctcct gcgtggccca ggctggaacg atgttgctgt tcgccagcag cacgcgcggc
      961 gcgtcggcat gggtgcggaa cacgcccacc ggcttgcccg actggatcag cagcgtctcg
     1021 tcgtcttcga gcgtgcgcag catctccagg atcttgagca gcgcgggcca gctacgcgcc
     1081 gcccgcccgc gcccgccgta gaccaccagc tcgtccgggc gctcggccac ctccgggtcg
     1141 aggttgttct gcaacatgcg ataggccgct tcctgcaacc agcccttgca gttgagcgtg
     1201 gccccgcgcg gcgcgcgcat gggccggggc gccacctggg cgtcctgggc gggaacggca
     1261 ttggcgcgtc cgacagtact ggtcatggtg atcctccttc attcgcgatg gcggcttcca
     1321 cctcgcgcac cagccgcccg ctgctgatca gctcgtcggc ccaggcgatg tcggcggtga
     1381 acggggcgtc ctccgtgcgg aaggccacgc gctcgcgcag cagatcgtag gccacctggg
     1441 tgccgcggcc caggcgcaag ggccggcccg ccgcctcccg gcgcaggtcg atggcctggc
     1501 ccgcggtcag cagttccatc gccagcacca gcgtcgtgtt gcgcatgatg gcccgcgcgt
     1561 ggcgcgcggc aatcggcccc atgctcacgt ggtcctcctg gttggccgag gtggggatcg
     1621 agtccacgct ggccgggtgg gccaggctct tgttctccga aacgagggcg gcggcgctgt
     1681 actgcaccat catgaagccg gagttcagcc cgccgttggt gatcaggaag ggcggcagga
     1741 taccgccgtt ctcgtggaca tcggtcaggc gggcctggcg ccgctcgctg atcgaggcca
     1801 cgccgcagaa ggggatgccc aggaagtcgg cggccacggc caccggcatg ccgtggaagt
     1861 tgccgcccga cagtacggcg acctcgtcgt ccatgcccac ggcgcccgcg ccgaagatga
     1921 gcgggttgtc cacggcggcg ttcagttcgg tctccacgat gccgcgcaca taggccaggg
     1981 cgtcgctggc cgggccgtgg acctggggca tgcagcgcag cgagtaggcg tcctgcacct
     2041 tgtgcgccgc cgggtccggt ccaccgctgc ccgcggcatc gggcgcccgc agcgccacat
     2101 ggtcctgcca gacgaactcg ctgcccgcca gcagtgcgcg cagatgcgcg gcggcggcga
     2161 tctgcccgcc ctggccgcgc gcggcgtgga tgcgcgggtc gagcgcggcc gggatgccct
     2221 gcaacgcctc gaacgacagt gcgccgcaga tgtcggcggc cagcgccagg ttggccgcgt
     2281 cggcggtggc gagggccagc agcccgctga tctgcgaggt gccgttgcag agcgccagcc
     2341 cctccttggc gatcagttcg accggggcca gccccgccgc ccgcaaggca tcgcaggccg
     2401 gttgcagggt gccctggtat tcggccaggc cgccgcccat catcgcgtgg gccatgtgcg
     2461 agagcggggc caggtcgccg ctggcgccga ccgagccttg cgccgggatg acagggtgca
     2521 cgccgcggtt gatcatgtcg atcagcagtt gcagcgtgcc gcgccgcaag cccgaatagc
     2581 cgagggccag cgtctgcgcg cgggcgatca tcagcgcacg ggtttcctcg cggctcaggg
     2641 cggggccgac gccgaccgtc tggctggtga tcaggttggc ttgcaggcgg gccaggtcgg
     2701 gcaggggaat cacgcggtcc ttgaaggcgc cgaagccggt ggtgatgccg tagaccacct
     2761 gccggctgtc cacg
//
LOCUS       DASUYC010000919         2645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1801997, whole genome shotgun sequence.
ACCESSION   DASUYC010000919 DASUYC010000000
VERSION     DASUYC010000919.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1801997"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..1462)
                     /locus_tag="VF276_15040"
     CDS             complement(113..1462)
                     /locus_tag="VF276_15040"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6608218.1"
                     /translation="MLYLSQLLGRPLRDRHGNQVARIQDLVVRLPAPAAEITGAARYQ
                     QYPRLHGLVAKGGKHATGFFVPVASLGDLSHDGGRLMTPDVFLETFARRPGELLLAAD
                     LMDHPVIDCRRAVVRRVNDVLIGLPAEEGQTHLPAPAAGAVTAYPAASLVLLGVDVGW
                     TGLLRRLNLLSSTTGVLRALGKRLAPSVLPWPDLALTGPGHGGRGPALRGLHPADIAR
                     LTDALSYHEAAGVIAGLDDETAADVLEEVDPDRATDIMEQLPDARAADIIEEMGPDEA
                     SDLLGDLPEDRAADLLRAMEPEAADDVRELLRYPDDSAGGMMTTSFVTCPHDYSVAQA
                     IALLRTELEKPDLIYYIYVTSDPENAHLAGVVTLRKLLIAAPDERMETIMRTDFEHAG
                     PYDNPRDVARTMAEYNLLALPVLDDTGRLLGIITVDDAIEVLLPSGWQRKLNRIFNS"
     gene            complement(1501..2607)
                     /locus_tag="VF276_15045"
     CDS             complement(1501..2607)
                     /locus_tag="VF276_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008700835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX6608219.1"
                     /translation="MKAVAVFPKKPNTMHLADLPEPRLEDVPNGRGVLVKVLRVGVDG
                     TDKEINAGEYGAAPPGYDFLVTGHESFGRVLAVGPNVMGLQVGDYVVATVRRPGHSIY
                     DLIGTYDMTTDDVYYEHGINLLHGFLTEQYVDDPEYIVKVPAGLKDIGVLLEPCSVIE
                     KGIAQAYEIQRRLRVWRPQRAAVLGAGSVGLLATMGLRLRGLEVVTLARTPPPTIHSK
                     LVEDIGAHYISTKQTSLADAAKEHGPFDIIFEATGYAPLAFEAMEILGKDGVLVLSSV
                     TGGDTKVEVPADRINLGFVLGNKVMVGTVNANREYFEMGVRDFAMAEAGYPGWLGRLL
                     TNKVEGLDNYAEMLRLLTEDNEAIKVYVEVAADT"
BASE COUNT          399 a          907 c          897 g          442 t
ORIGIN      
        1 gcccgcaggc gcccacgcca cccccggcgc ggcgccggac gtgtcgattc gccgggggat
       61 gcctcctcga ctggttgcgt aactccgtga gccatacttt gcctgggtgc cgctaggaat
      121 tgaagatgcg attgagcttg cgctgccagc cgctgggcag caaaacttcg atcgcgtcgt
      181 ccacggtgat gatgccgagc agccggccgg tgtcgtccag cacgggcagc gccagcaggt
      241 tatactcggc catggtgcgg gccacgtcgc gcgggttgtc atacgggccg gcgtgctcga
      301 agtcggtgcg catgatcgtc tccatgcgtt cgtcgggggc cgcgatcagc agtttgcgta
      361 acgtaaccac cccggccaga tgggcgttct ccgggtcgct ggtgacgtag atgtagtaga
      421 tcaggtcggg cttttcgagt tccgtccgca gcagggcgat agcctgggcc acgctgtagt
      481 cgtgcgggca ggtgacgaaa gaggtcgtca tcatcccacc ggcgctgtcg tcgggatagc
      541 gcagcagttc gcgcacatcg tcggcggctt ccggctccat ggcgcgcaac aggtccgccg
      601 cccggtcttc aggcaggtcg ccgagcaggt cgctggcctc gtccggcccc atctcctcga
      661 tgatgtcggc ggcgcgggcg tcgggcagtt gttccatgat gtcggtggcg cggtcgggat
      721 ccacttcctc cagcacgtcg gcggcggttt cgtcgtccag cccggcgatg acccccgctg
      781 cctcgtggta gctgagggcg tcggtcaggc gggcgatgtc ggcggggtgc aggccgcgca
      841 aggccgggcc gcgcccgccg tgccccggcc ccgtcagcgc caggtcgggc cagggcaaca
      901 cgctgggcgc cagccgtttg cccagcgccc gcaggacgcc ggtggtgctc gacagcagat
      961 taagccgccg caacagcccg gtccacccca cgtccacccc cagcagcacc agggacgccg
     1021 ccgggtaggc cgtcacggcg ccggcggcgg gcgcgggcag atgcgtctgg ccctcctcgg
     1081 cgggcaggcc gatgagcacg tcgttgaccc ggcgcacgac ggcccggcgg cagtcgatca
     1141 ccgggtggtc catgaggtct gcggccaaca gcaattcgcc ggggcggcgg gcgaacgtct
     1201 ccaggaagac atccggggtc atcagccggc ccccgtcgtg gctcaggtcg cccagcgagg
     1261 ccacgggcac gaaaaagccg gtggcgtgct tgccgccctt cgccaccagg ccgtgcagcc
     1321 ggggatactg ctggtaacgc gccgcacccg tgatctcggc ggcgggcgcg ggcaaccgga
     1381 ccaccagatc ctggatgcgc gcgacctggt tgccgtggcg gtcgcgcagc gggcggccca
     1441 gcagttggga gagatacagc atagcaatgg ctattataca cccgcgcgca cgggcagcgc
     1501 tcaggtatcg gccgcgacct cgacgtagac tttgatcgcc tcgttgtcct cggtcaacag
     1561 gcggagcatc tcggcgtagt tatccagccc ctccactttg ttagtgagca agcggcccag
     1621 ccagcccgga tagccggcct cggccatggc gaagtcgcgc acacccatct cgaaatactc
     1681 gcggttggcg ttcaccgtgc cgaccatgac cttgttgccc agcacgaagc ccaggttgat
     1741 gcggtcggcg ggcacctcga ccttggtgtc gcccccggtg acgctgctca ggacgagcac
     1801 gccgtccttg cccaggatct ccatcgcctc gaaggccagc ggggcgtagc cggtggcctc
     1861 gaagatgatg tcgaaggggc cgtgctcctt ggccgcgtcg gccagcgagg tctgcttggt
     1921 gctgatatag tgcgcgccga tgtcctcgac cagtttgctg tggatggtcg ggggcggcgt
     1981 gcgggccagc gtcacgactt cgaggccgcg caggcgcagg cccatcgtcg ccagcaggcc
     2041 gacgctgccc gcgcccagca cggcggcccg ctggggccgc cacacgcgca gccgccgctg
     2101 gatctcatac gcctgggcga tgcccttctc gatcacgctg cacggctcca acagcacgcc
     2161 gatatccttc agcccggcag gcaccttgac gatatactcc gggtcgtcca catactgctc
     2221 ggtcaggaag ccgtgcagca gattgatgcc atgctcgtaa tagacatcgt cggtggtcat
     2281 gtcgtaggtg ccgatcaggt cgtagatgct gtggccgggc cggcgcacgg tcgccacgac
     2341 gtaatcgccc acctgcaaac ccatcacgtt cggcccgacg gccagcaccc ggccaaagct
     2401 ctcgtgcccc gtgaccagaa aatcgtaacc gggcggcgcc gcgccgtact cgccggcgtt
     2461 gatctccttg tcggtgccgt ccacgcccac gcgcaacacc ttgaccagca cgccgcggcc
     2521 attgggcacg tcttccagcc ggggttcggg caggtcggcc aggtgcatcg tgtttggctt
     2581 cttggggaat actgcgactg ctttcatagc ggcctcctct ctaccgccat cctataacgc
     2641 gggcg
//
LOCUS       DASUYC010000920         3919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1307539, whole genome shotgun sequence.
ACCESSION   DASUYC010000920 DASUYC010000000
VERSION     DASUYC010000920.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307539"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VF276_15050"
     CDS             complement(<1..693)
                     /locus_tag="VF276_15050"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HEX6608220.1"
                     /translation="MPAGSERWAARAPAAPAYLAYHEDGFVPGLPLARLGTPDARPAG
                     PPVAVPAASLRPAYAACAAMIRAHSASFYFSASLLPPAKRQAVLALYAFCRTSDDLVD
                     SAPARGLSVAETEQALADWAARCRAPHPARDETAPVVLAWADTRRRFAIPQALADELL
                     AGVRMDISIARYATWADLWIYCYRVASTVGQMSMHITGAIHPAATNYAIRLGVALQLT
                     NILRDVGEDARQG"
     gene            complement(680..2119)
                     /locus_tag="VF276_15055"
     CDS             complement(680..2119)
                     /locus_tag="VF276_15055"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6608221.1"
                     /translation="MHVLIAGGGLAGLAAAKYLSDTGHRVTVVEKRAEAGGKVSSWQD
                     AGGDWLESGLHVFFGAYRNLHRLFRETGLAANLDWKAHEMTFSRPGGWLSPLRFARGL
                     PAPAHGLVAIARNRGVLTLPDKLRVGYGLLWPIAGNQAWIDAQDRYTYAQWHLRHRMG
                     PRALGDFFDTMALALNFDTTERVSAKLVLTVLSHFAKETDASRVAFLRGTPQRRLFEP
                     FLAYLARRGVRFVPNSKVAGIEYDAAMNRVTGFRLTGGELLQADCYVSAMPVHNLWKT
                     LPEALRAQPPFADLRRLHGQPVMTVQLYFDRPVIGVDNLIFSSGTHLSVYAELSRCAA
                     DLHEGPAGRHMVEMVVAPAAEWYRLDDADVIRRTLAEFTQLHPQAAQAHLLKSTLVRI
                     PQSVYRARPGHDRYRPDQATPVPNFYLCGDYTRQDYLASMEGAVLSGKRVAERIVAGT
                     GVVAGPGAGARWGLTGGAPLAEHAAVAGR"
     gene            2319..2945
                     /locus_tag="VF276_15060"
     CDS             2319..2945
                     /locus_tag="VF276_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608222.1"
                     /translation="MNPVLRYCALPGAPARRTDTAQMLGKVGAQAAFHRLAARHLAAL
                     SAAPVPCTALTVACFREDGALLTWRFRRDQLWDLPSCALPPGADAAQYAAHVLTDLGG
                     VTPAERLQVFGLVDWGPAARAAGWGWTACLFGQVGAVDPLPRPADVSGRAFLPVARIP
                     PGVPADPWPRVRSALLQAATEVFAATHDFWLLQTWDALIMYDLDDVPG"
     gene            3048..3121
                     /locus_tag="VF276_15065"
     tRNA            3048..3121
                     /locus_tag="VF276_15065"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3080..3082,aa:Gln,seq:ttg)
     gene            complement(3417..3488)
                     /locus_tag="VF276_15070"
     tRNA            complement(3417..3488)
                     /locus_tag="VF276_15070"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3453..3455),aa:Arg,seq:ccg)
     gene            3590..>3919
                     /locus_tag="VF276_15075"
     CDS             3590..>3919
                     /locus_tag="VF276_15075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608223.1"
                     /translation="MTSIALDVHMIGARETGNETYATELAAALGRLGGYQYLLYTPHP
                     DALPARLRAQPNLAARPFANLPSAVRIPWLYPRLVRADAARLLHMMYVAPPYVTCPVV
                     LTVHDVSY"
BASE COUNT          581 a         1307 c         1392 g          639 t
ORIGIN      
        1 accctggcga gcgtcttcgc ccacgtcgcg cagaatgttg gtcagttgca gcgccacgcc
       61 gagccggatg gcgtagttcg tggccgccgg gtggatggcc ccggtgatat gcatgctcat
      121 ctgccccacg gtggaggcga cgcgatagca gtagatccag aggtcggccc aggtggcgta
      181 gcgggcgatg gaaatatcca tgcgcacccc ggccagcagt tcgtcggcca gggcttgcgg
      241 gatggcgaag cgccggcggg tgtcggccca ggccagcacc acaggcgccg tttcgtcccg
      301 cgccgggtgc ggcgcccggc agcgcgcggc ccaatccgcg agggcctgct cggtctcggc
      361 caccgagagg ccgcgggcgg gggcgctgtc caccagatca tcgctggtgc ggcaaaaggc
      421 gtagagggcc agcacggcct gccgtttggc gggcgggagc aggctcgcgc tgaaatagaa
      481 gctggccgag tgcgcgcgga tcatggcggc gcacgccgcg taggccgggc gcaagctcgc
      541 ggcgggcacg gcaacgggcg ggccggcggg gcgggcgtcc ggcgtgccga gtcgcgccag
      601 gggcaggccg gggacgaatc cgtcctcgtg gtaggccagg taggcgggcg cggcgggggc
      661 gcgcgcggcc cagcgctcgc taccggccgg caacggcagc gtgttcggcc agcggcgctc
      721 cgccggtgag tccccagcgc gcgcctgccc ctggtccggc caccacgccg gttccggcca
      781 cgatccgctc tgcaactcgt ttgccactga gcactgctcc ttccatactc gccaggtagt
      841 cctgccgggt gtaatcgccg cacagataga agttcgggac gggcgtggcc tggtcgggcc
      901 ggtagcggtc gtggccgggg cgggcgcggt agaccgattg ggggatccgc accagggtgc
      961 ttttgagcag gtgcgcctgg gccgcctgcg ggtggagttg ggtgaactcg gccagcgtcc
     1021 ggcggatcac gtccgcgtcg tccaggcggt accactcggc ggcgggcgcc acgaccatct
     1081 cgaccatgtg gcggcccgcc ggtccctcgt gcaggtccgc cgcgcaccgg ctcagttcgg
     1141 catagaccga gaggtgcgtg ccgctgctga agatcaggtt gtcgacgccg atcaccgggc
     1201 ggtcaaaata aagctgaaca gtcatcacgg gttggccgtg cagccggcgc aggtcggcga
     1261 aggggggctg ggcccgtaac gcctccggca gcgttttcca gaggttatgc accggcatcg
     1321 ccgagacgta gcaatcggct tgcagcagtt cgccgccggt cagccggaac ccggtcacgc
     1381 ggttcatcgc tgcgtcatat tcgatgccgg ccactttgct gttgggcacg aagcggaccc
     1441 cccgccgggc taggtaggcc aggaaaggct cgaagaggcg gcgctggggc gtcccgcgca
     1501 ggaaagccac gcgcgaggcg tcggtctcct tggcgaagtg cgacagcacg gtgagcacca
     1561 gcttggcgct gacccgctcg gtggtgtcga agttgagggc cagggccatc gtgtcgaaga
     1621 agtcgcccag cgcgcgcggt cccatgcggt ggcgcaggtg ccattgggcg taggtgtagc
     1681 ggtcctgggc atcgatccag gcctggttgc cggcaatggg ccagagcagg ccgtagccca
     1741 cgcgcagctt atcgggcagc gtgagcacgc cgcggttgcg ggcgatggcg accagcccgt
     1801 gggcgggggc ggggagcccg cgcgcgaagc gcagcggcga cagccaccca ccgggccgcg
     1861 agaaggtcat ttcgtgcgcc ttccagtcca ggttggcggc gaggcccgtt tcccggaata
     1921 gccggtgcag gttgcggtag gcgccgaaga acacgtgcag gccgctttcg agccagtcgc
     1981 ctccggcatc ttgccaggac gagactttgc cgcccgcctc ggcccgcttt tcgaccaccg
     2041 tgacccggtg cccggtatcg ctcaggtatt tggcggccgc cagtccggct aacccgccgc
     2101 cggcaatcaa cacgtgcatg gacgtggtcc ctctcctgct tacaccacta caggcatcac
     2161 aagtgataca ctgccgccag tataatggat agctggatct ttgtcaatac tttgttcaat
     2221 catttggtag ttgcagtgcg tataccagtg cgctataatg ccgcagacaa tagcccagtt
     2281 gtgacgctgg acacggcgcg ggcagcgagg aaccgccgat gaatcccgtt ctacgatact
     2341 gcgcgctgcc cggcgcgccg gcccggcgca ccgatacggc ccagatgctg ggcaaggtcg
     2401 gtgctcaggc ggcgttccat cgcctggcgg cccggcacct ggccgctctt tccgccgcgc
     2461 ccgtgccctg caccgccctg accgtcgcct gctttcgcga ggatggggcg ctgctcacct
     2521 ggcgattccg gcgcgaccag ctgtgggatc tcccctcctg cgccttgccg cccggcgccg
     2581 atgccgcgca gtacgccgca cacgtgctca cggatctcgg cggggtcacg cccgcggaac
     2641 gcttgcaggt tttcggcctg gtcgattggg ggccggcggc ccgcgccgcc gggtggggct
     2701 ggacggcctg cctgttcggg caggtcggcg ccgtcgatcc cttgcccagg cccgcggacg
     2761 tgagcgggcg tgctttcttg ccggtggcgc ggataccgcc cggcgtgccg gcggatccct
     2821 ggccgcgggt gcgcagcgca ttgctgcagg cggcaacgga ggtgtttgcg gcgacgcacg
     2881 acttctggct attgcaaacg tgggacgcgc tgataatgta cgatctcgac gatgtaccgg
     2941 ggtaagcttc cgtcacgggg gcgggcgcgc ggtgggggcg tgtggagcgg cccggacagg
     3001 ctttgacaag cgagggccgg atcttgtata gtggtaccag actactttcg gggatcgtct
     3061 aatggtagga caccagcctt tggagctgag agtcggggtt cgaatccctg tccccgaacc
     3121 acctgccgta ccggccccgc ctggccggaa accatgcttt gctaccgacc cgtccgctcg
     3181 cgatagttgt accgctatcg ccctgccgga tcgggtcgct tgctttcccc ttccgcactg
     3241 atccaccgcg ccgggaacct gggcgccccc ggtaggccac aggaggagac acggtatgcg
     3301 cagcgttcgg gtgcccggcc ggcgtgtcgc cggcgctcac cgggcagagc ggggcgggca
     3361 aaacaaaacc gccgggttct cagcacgaga ccggcggttt gccaatgcat aacgggtacg
     3421 cctggaggga ctcgaacccc cgcatccagc tccggaggct ggcgctctat ccactgagct
     3481 acaggcgcag atatgaccac gcctattata ccatgtgctg cccgcgctgg caagcgttac
     3541 cggccccgcg ggtcctcgac ttttcgcgcc atcccgaaag gacccgcgga tgaccagtat
     3601 cgcgctcgac gttcacatga tcggcgcccg cgaaacgggc aacgaaacct acgcgaccga
     3661 actggcggcg gcactgggcc ggctgggcgg ctaccagtac ctgctttata cgccgcatcc
     3721 ggatgcgctg ccggcgcggc tgcgcgcgca gccgaacctg gcggcgcgcc ccttcgccaa
     3781 tctgccgtcg gcggtgcgca tcccctggct ctatccccgc ctggtgcgcg ccgacgctgc
     3841 ccgcctgctg catatgatgt atgtggcgcc gccctatgtg acctgcccgg tggtcctgac
     3901 cgtgcatgac gtatcctat
//
LOCUS       DASUYC010000921         2056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_615530,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000921 DASUYC010000000
VERSION     DASUYC010000921.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615530"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="VF276_15080"
     CDS             complement(<1..992)
                     /locus_tag="VF276_15080"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009595907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX6608224.1"
                     /translation="MTDTPALPRSTPEAQGIPSAAILAFVEAVDRDLDALHSFMLLRH
                     GQVVAEGWWDPYRPADPHTLFSLSKSFTSTAIGLLVAEGRLAVDDPVLDFFPGDAPPE
                     PSANLRAMRVRHLLTMTTGHAKDTTGPMAEQPGRTWVQGFLAQPVEYAPGTHFLYNTG
                     ATYMLSAIVQKLTGGRMIDYLQPRLFARLGIANPRWETSPQGIDVGGWGLSTTTADIA
                     AFGQLYLQRGMWQGARILPETWVDAATAPQVDNGPADNPDWGQGYGYQFWRCRHDAYR
                     GDGAFGQYCVVMPDQDAVLAITGGLDDMQPPLDLVWQHLLPAMGAAPLPDDKAA"
     gene            complement(1219..1581)
                     /locus_tag="VF276_15085"
     CDS             complement(1219..1581)
                     /locus_tag="VF276_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6608225.1"
                     /translation="MVDHPILVIDDDPGILSTVTDILEFEGYAVVQAGNGAEGLALVE
                     QVHPSLILLDMRMPVLDGWGFARRLREQGSGPPIVVMTAAQDARRWAQEIGAAGFVAK
                     PFEVDDLLATVEGLRPPA"
     gene            complement(1574..>2056)
                     /locus_tag="VF276_15090"
     CDS             complement(1574..>2056)
                     /locus_tag="VF276_15090"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6608226.1"
                     /translation="HPCRVDAAGPVTGVYDPVRMMQLIENLVENAVKYSPEGGPVRVQ
                     VWQEAGTNHLSVTDSGIGIPTEDLPHVFERFHRGTNVDDRRFAGMGLGLFICRGIAEQ
                     HGGHIWATRAPGRGSTFHVALPVAAPPGESEPGAAPTQEATPVAAAPAGPAQGGASHG
                     "
BASE COUNT          297 a          711 c          734 g          314 t
ORIGIN      
        1 tgcgcggcct tgtcgtccgg cagcggcgcg gcgcccatgg ccggcagcag gtgttgccag
       61 accaggtcga gcggcggctg catatcgtcc agcccgccgg tgatcgccag gacggcgtcc
      121 tggtcgggca tgaccacgca gtactggccg aaggccccgt cgccgcggta ggcgtcgtgg
      181 cggcagcgcc agaactggta gccgtaaccc tgtccccagt cggggttgtc cgccgggcca
      241 ttgtccacct ggggcgcggt ggccgcatcc acccaggttt ccggcaggat tcgggcgccc
      301 tgccacatgc cccgctgtaa gtagagctgg ccgaaggcgg cgatgtccgc cgtggtggtg
      361 ctcaagcccc agccacccac gtcgatgccc tgcggcgagg tctcccagcg tgggttggcg
      421 atgcccagcc gcgcgaacag gcgcggctgc aggtagtcga tcatgcgccc gccggtcagc
      481 ttttgcacga tcgccgacag catgtaggtc gcgccggtgt tatagagaaa gtgcgtgccc
      541 ggcgcatact cgacgggctg cgccaggaag ccctgcaccc aggtgcggcc cggctgctcg
      601 gccatcggcc ccgtggtatc cttcgcgtgg ccggtggtca tggtcagcag atggcgcacg
      661 cgcatagccc gtaagttggc actcggttcc ggcggcgcgt cgccggggaa gaagtccagc
      721 accgggtcgt ccaccgccag ccggccctcg gccaccagca acccgatggc cgtcgaggtg
      781 aagctcttgc tcagcgagaa cagcgtatgc gggtcagccg ggcggtaggg gtcccaccac
      841 ccttcggcga ccacctggcc gtggcgcagc agcatgaagc tgtgcagcgc gtcgaggtcg
      901 cggtccaccg cctcgacaaa ggcgaggatc gcggcggagg ggatgccctg ggcttcgggc
      961 gtgctgcgcg gcaaggcggg tgtgtcggtc atttggcggc tcctactggg atgatacggg
     1021 cggcgtggcg cgggccggtt tcaaaccggg ccctacacca gcatctcggt ataagttgtg
     1081 gccctacggc aatgcgccgg cgggccggtt tgcatccggc tcctacaccg gcacagcgtt
     1141 acgggtagaa atacaggtgc gggccggttt gcatccggca cctgcgccgg gcaagcgcgg
     1201 cggcgggcta cccgcgggtc acgcgggcgg gcgcaggccc tcgacggtcg ccagcaggtc
     1261 gtccacctcg aacggcttgg cgacaaagcc cgcggcgccg atctcctggg cccagcggcg
     1321 ggcatcctgc gccgccgtca taaccacaat gggcggaccg ctgccctgct cgcgcaagcg
     1381 ccgggcgaag ccccacccgt cgagcaccgg catgcgcata tccagcagga tcaggctcgg
     1441 atgcacttgc tccaccagcg ccagcccctc ggccccgttg ccggcctgga cgacggcgta
     1501 gccctcgaat tcgaggatgt cggtcacggt ggacaagatg ccgggatcgt cgtcgatcac
     1561 caggatggga tggtcaacca tggctagcgc ctccctgcgc tgggccggct ggcgccgccg
     1621 cgaccggcgt agcctcctgg gtgggggccg cgcccggttc cgactcgccg ggcggggccg
     1681 cgacgggcaa ggccacatgg aacgtgctgc cgcggcccgg cgcgcgggtg gcccagatgt
     1741 gcccgccgtg ctgctcggca atgccccggc agatgaacag gcccaggccc atcccggcga
     1801 agcgccggtc gtctacgttc gtgccgcgat ggaagcgctc gaacacgtgc ggcaggtcct
     1861 cggtcgggat gccgataccg gaatcggtca cgctgagatg gttggtgccg gcctcctgcc
     1921 agacctgcac ccgcaccggc ccgccctccg ggctgtactt gacggcgttc tccaccaggt
     1981 tttcgatcag ttgcatcatg cgtaccgggt cgtagacgcc cgttaccggg ccggcggcgt
     2041 ctacccggca cgggtg
//
LOCUS       DASUYC010000922         3418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1142521, whole genome shotgun sequence.
ACCESSION   DASUYC010000922 DASUYC010000000
VERSION     DASUYC010000922.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142521"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..2102
                     /locus_tag="VF276_15095"
     CDS             81..2102
                     /locus_tag="VF276_15095"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX6608227.1"
                     /translation="MQATFRFPLIMLLALALGLALLPARAGAQTPPTRSITLNQLTGS
                     GWPTVRVNLNLRSLNNTALGDVGPDQFVVEENGVAQHIGGIALGAASGAPLSVVLVLD
                     TSGSMAGPKLAAAQAAGSAFLKALGPQDAAALVPFNTQVGAVTAFTTDKDQVAAALNA
                     QTAAGNTAVYDALKTAALLAGGAPEGNRRAVVLLTDGRDTSSRSLPDLGLATAKQHDV
                     LVYTIGVGTDTDDRVLTGLAEPTGGRYIKATDPATLKGIYEELAKELTGQYLLTYTST
                     TRVTKNYETILVRVRYTAPGGEAIVQEIRYRPPPAALTAPTPVPTITPVPIAQVAIPA
                     GLVQAKADPPAQQGTAAPADPSAGLRLLLTVLSALAALLAIIAVLLIVGALIVSRSPT
                     AMKARVDRFVAETDMGVPGNTPPPGFSARVLYPFFNGLGRRLGAFTPARYLDQIQALL
                     YQAGPPYRMTRTGFLGLQAGWGLVLALLALLWAMLLSHQPPFMWLVLAALGLAIGLYL
                     PYFSLARRVTERKKRVLRAMPAALDFMAIMVEAGMGFDAALNELVRRWRNTLTDEFAL
                     LLIDFQIGKPRRTAWQELSHRTEVPDLNSFVVAMIQSEQTGISIGDLLRTQAEQIRIR
                     RRQRAEEEARMAPVKMLIPMALLIFPTILMVILGPALPQLFGTINGLSH"
     gene            2256..>3418
                     /locus_tag="VF276_15100"
     CDS             2256..>3418
                     /locus_tag="VF276_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX6608228.1"
                     /translation="MERDAALSLARILPRLEARIARDADPAAWATFRARLETHFGTLF
                     PLLRHLYGGLYDFFYHLESTLEMAARMWLARPAELQALDADREAHPDWYQSERMLGAV
                     CYVDLFAGDLAGVRAQIPYFQELGVTYLHLMPPFLAPAGNNDGGYAVSSYRDINPALG
                     TMDEMRDLATELRHHGISLALDFVFNHTADEHTWALHALAGDPDYQDYYRMFPDRQMP
                     DAYERTLREIFPEQRPGSFTWRPDAGRWVWTTFNSFQWDLNYANPVVFTRMAEEMLFL
                     ANQGVEILRLDAVAFIWKQLGTNSENLPEAHMLIRAFNTIVRLAAPSMLFKSEAIVHP
                     DEVVKYIRPDECQLSYNPLLMALLWNSLATRDVRLLQQSMRERFAIDPRCAWV"
BASE COUNT          546 a         1256 c         1046 g          570 t
ORIGIN      
        1 gaacaagatg gcgtccatcg atgtctaggc ccaatgcgac cccgcgccgt cccggcgccc
       61 cacgatacag aggatgggcc atgcaggcta ctttccgctt cccgctgatc atgctgctgg
      121 cgctcgccct gggcctggca ctcctgcccg cccgcgccgg cgcgcagacg cctccgaccc
      181 gcagcataac gctcaaccag ttaaccggat cgggctggcc caccgtgcgc gtcaacctga
      241 acctgcgcag cctcaacaac accgcgctcg gcgatgtcgg ccccgatcag tttgtggtcg
      301 aggagaacgg cgtcgcccag cacattggcg gtatcgccct cggcgcggcg agcggggcac
      361 cgctctcggt cgtgctggtg ctcgacacga gtggcagcat ggcggggccg aaactggccg
      421 cggcccaggc ggcgggcagc gccttcctca aggcgctcgg gccgcaggat gccgcggccc
      481 tggtaccctt caatacccag gtcggggctg tgacggcttt cactaccgac aaagaccagg
      541 tggccgccgc gctcaacgcc cagaccgccg ccggcaatac ggccgtctac gacgccctga
      601 aaacggcggc cctgctggcc ggcggagcgc cggagggcaa ccgccgcgcc gtggtgctgc
      661 tcaccgacgg ccgggacacc agcagccgca gcctacccga tctcggtctg gcgaccgcga
      721 agcagcacga tgttctggtc tacaccatcg gcgtcggcac cgacacggac gacagggtgc
      781 tgacggggct cgctgagccg acgggaggcc gctatatcaa ggccaccgac ccggcgacgt
      841 tgaagggcat ctacgaggaa ctggcgaagg aactcaccgg ccagtacctg ttgacctaca
      901 cctcgaccac gcgcgtcacg aagaactatg agaccatcct ggtgcgtgtg cgctatacgg
      961 cgccgggcgg tgaagcgatc gtgcaggaga tccggtaccg cccaccgccc gccgcgttga
     1021 ccgcgccgac gcccgtgccg acgatcacgc ccgtgccgat cgcccaggtg gcgatcccgg
     1081 cgggactagt gcaggccaaa gccgaccccc cggcccagca gggcacggcg gcgcccgctg
     1141 atccatcggc ggggctgcgc cttctgctga cagtgctcag cgcgctggcg gcgctgctgg
     1201 ctatcatcgc cgtgttgttg atcgtgggcg cgctcatcgt ctcgcgctcg cccaccgcga
     1261 tgaaggcccg cgtcgaccgc tttgtcgccg agaccgatat gggcgtgccc ggcaacacgc
     1321 ccccgcccgg tttcagcgct cgtgtgctct atccgttctt caacgggtta ggccggcgcc
     1381 tgggcgcctt cacccctgcg cgctacctcg accagatcca ggcattgctc tatcaggccg
     1441 ggccgcccta ccgtatgacc cgcaccgggt tcctggggtt acaagccggc tgggggctgg
     1501 tgctggccct gctggccctg ctgtgggcga tgctgctctc gcaccaaccg cccttcatgt
     1561 ggctggtcct ggcggccctc ggtctggcga tcgggctcta cctgccctac ttctcgctgg
     1621 cccggcgcgt gaccgagcgg aaaaagcgcg tcctccgcgc catgcccgcg gcgctcgatt
     1681 tcatggcgat catggtcgaa gccggcatgg gcttcgatgc tgccctgaac gaactcgtgc
     1741 gccgctggcg caacacgctg accgacgagt ttgcgctctt gctgatcgac ttccagatcg
     1801 gcaaaccgcg ccgcaccgcc tggcaggagc tttctcaccg cacggaggtg cccgacctga
     1861 actcgtttgt cgtcgccatg atccagagcg agcaaaccgg catcagcatc ggcgatctgc
     1921 tgcgcaccca ggccgagcaa atccgcatcc gccgccgcca gcgcgcggaa gaagaagcgc
     1981 gcatggcccc ggtgaaaatg ctgatcccca tggccctgct catcttcccg accatcctca
     2041 tggtcatcct gggcccggcg ctgccccagc ttttcgggac catcaacggc ctctcgcact
     2101 agaatcggcc cgcccgcgcc gcccgtctcc cgctttatcg ggggatgggc ggtgtatctt
     2161 cgggctaccc cggcttcttt tgccgcccac ggcaacaact tcggcggcat cccgtttagg
     2221 atatagcacc atcctgacta cgggagtatc cacctatgga acgagacgcc gccctgtccc
     2281 tggcgcgcat cctgccgcgc ctggaagccc gcatcgcccg cgacgccgac cccgccgcct
     2341 gggccacctt ccgcgcccgg ctggagaccc atttcggcac cctctttccg ctcttgcgcc
     2401 acctgtacgg cgggctgtat gatttcttct accacctgga gagcacgctg gagatggccg
     2461 cgcgcatgtg gctggcccgg cccgccgagt tgcaggcgct cgacgccgac cgcgaggccc
     2521 atcccgattg gtatcagtcg gagcgtatgc tgggcgccgt ctgctacgtc gatctgttcg
     2581 cgggcgatct ggccggtgtc cgggcgcaga tcccctactt ccaggagctg ggtgtcacct
     2641 atctccacct gatgccgccc ttcctggccc cggcgggcaa caacgacggg ggctacgccg
     2701 tcagcagcta ccgcgacatc aacccggcgc tcggcaccat ggacgagatg cgcgacctgg
     2761 cgacggaatt gcgccaccac ggcatcagcc tggcgctcga cttcgtcttt aaccacacgg
     2821 cggacgagca tacctgggcg ctgcacgccc tggccggcga ccccgactac caggactact
     2881 accgcatgtt ccccgaccgg cagatgcccg acgcctacga gcgcaccctg cgtgagatct
     2941 tccctgagca gcgccccggc agctttacct ggcggcccga cgccggccgc tgggtctgga
     3001 ccaccttcaa cagcttccag tgggacctga attacgccaa ccccgtcgtc ttcacccgca
     3061 tggccgagga gatgctcttc ctggcgaacc agggagtgga gatcctccgc ctcgacgccg
     3121 tggcctttat ctggaagcaa ctgggcacca acagcgagaa tctgcccgaa gcccacatgc
     3181 tcatccgggc gttcaacacc atcgtccgtc tggcggcgcc atcgatgctg ttcaagtccg
     3241 aggccatcgt ccaccccgac gaagtagtga agtatatccg ccccgacgag tgccagcttt
     3301 cgtataaccc gctgctcatg gccctgctct ggaactcgct ggcgacgcgc gacgtgcgcc
     3361 tgttgcaaca gtcgatgcgc gaacggttcg ccatcgaccc gcgctgcgcc tgggtgaa
//
LOCUS       DASUYC010000923         2354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1540437, whole genome shotgun sequence.
ACCESSION   DASUYC010000923 DASUYC010000000
VERSION     DASUYC010000923.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1540437"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..122)
                     /locus_tag="VF276_15105"
     CDS             complement(<1..122)
                     /locus_tag="VF276_15105"
                     /inference="COORDINATES: protein motif:HMM:NF039478.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6519 domain-containing protein"
                     /protein_id="HEX6608229.1"
                     /translation="MQGDITRSTFRPENHYDGVRMQQGRVQLDADWNEQVDIQQ"
     gene            complement(133..>2354)
                     /locus_tag="VF276_15110"
     CDS             complement(133..>2354)
                     /locus_tag="VF276_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608230.1"
                     /translation="YWFSPLGNDAALFTRPQTEREITQLADETNVPQPIRQAAFFADL
                     QSYQARYASVPPADRPADSIYYGPNGSLDIIADGEPVPPIAIMCMDLRNWDRPPAGRV
                     GVDVRRGRLAFATGETPNTVQVSYNYGFSADIGGGPYDRRRPPALGSAHGEDAPTEPD
                     TVAAPAALDALIRVPSTGINTLADALAAWDPATAPRTVIQIEDNRTYEEDLTIPMAGA
                     ELVIQAANRKRPVLLGDIAVTGGTGAESLTLNGLLVAGALNLPGNLAAVTIAHCTLVP
                     GRGLDETGAPRTPAAPSVIAAATNDRLQLTIDHSITGALRVSEEVAGLTVTDSIIDSP
                     TRGGRATVLPVLISSNLAPFPALGPDTPKVVNVTIGDDGPYPATLPGVPTTLADARDQ
                     LQTAIQAAHDSDAFTGARVISAANRLVVLPGAPGAVVIEPAGADNTATQLRLVASAAR
                     QVFALVGGSLAGFTGPQATAPQINVTMGGDGPHTATLATVPTTLAQARDQLQAAIQAA
                     DSAPAFTGTLVATLDDRLVVLPGTDRTEVVFSPTPGDATTAGDLALETDRPAIAASEG
                     GQAPGPPATLERATIFGATYVKELTLASEVIFTGVVLVEHRQAGCARFSYVPPASRTP
                     QRYRCQPDLEIAAQIAAAEQEAKAHNTVLTPAEAAAIAARVRAALRPAFTASRYGRAA
                     YGQLSAACPPQITTGAGDGAEMGVFNSLQGAVREANLRTALTEHLRFGLEAGIFYVT"
BASE COUNT          296 a          775 c          915 g          368 t
ORIGIN      
        1 tattgctgga tatcgacttg ctcgttccag tcggcgtcca gttgcacgcg tccctgctgc
       61 atacggacgc cgtcataatg gttttcgggc cggaaggtgg accgcgtgat atcgccctgc
      121 atggtactct ccttaggtga cgtagaagat cccggcttcg aggccgaagc gcagatgctc
      181 ggtcagggcc gtgcgcaggt tggcctcgcg cacggcgccc tgcaaactat tgaagacgcc
      241 catctccgcg ccgtcgccgg ccccggtggt gatctgcggc gggcaggcag cgctcaactg
      301 gccgtaggcg gcgcgcccat agcgcgaggc ggtgaaggcg gggcgcagcg ccgcccgcac
      361 gcgcgccgcg atggccgcgg cctccgcggg cgtgagcacg gtattatgcg ccttggcttc
      421 ctgctctgca gcggcgatct gggccgcgat ttccaggtcg ggctggcagc ggtagcgctg
      481 cggcgtgcgt gaggcgggcg gcacgtagct gaagcgcgca cacccggcct ggcgatgctc
      541 gaccagcacc acgccggtga agataacctc tgaggccagc gtcagttcct tgacataggt
      601 ggcgccgaag atcgtcgcgc gctccagcgt ggccgggggc cccggcgcct gaccgccctc
      661 gctggcggcg atggccgggc ggtcggtctc cagcgccagg tcgccggcgg tcgtggcgtc
      721 gccgggcgtg gggctgaaga cgacctcggt gcgatccgtg cctggcagca cgaccagccg
      781 gtcgtccagc gtggcgacaa gggtgccggt gaaggccggg gcactatccg ccgcctggat
      841 cgcggcctgg agctggtcgc gggcctgggc cagcgtggtg ggcacggtcg ccagcgtcgc
      901 ggtatgcgga ccgtcgccgc ccatggtgac gttgatctgc ggcgcggtgg cctgcggccc
      961 ggtgaagccg gccagcgacc cgcccaccag ggcgaacacc tggcgcgccg cgctcgcgac
     1021 cagccgcagt tgcgtcgcgg tgttgtcggc gcccgccggc tcgatcacga cggcccccgg
     1081 cgcgccgggc agcaccacca ggcggttcgc cgcgctgatc acgcgggcgc cggtgaaggc
     1141 gtcgctatcg tgggcggcct ggatcgcggt ctgcaactgg tcgcgggcgt cggccagcgt
     1201 ggtgggcacg ccgggcagag tggccgggta ggggccgtcg tcgccgatgg tcacgttgac
     1261 gaccttgggc gtatccggtc ccagcgccgg gaagggcgcc aggttgctgg aaatcagcac
     1321 gggcagaacg gtcgcccggc ccccgcgggt gggcgagtcg atgatgctgt cggtgacggt
     1381 cagccccgct acctcctcgg acacgcgcag ggcgccggta atgctgtggt cgatggtgag
     1441 ttgcagccgg tcgttggtcg cggcggcgat gacgctgggc gccgcggggg tgcgcggcgc
     1501 gccggtctca tcgaggcccc gccccggcac cagcgtgcag tgggcgatgg tcacggcggc
     1561 caggttgccc ggcaggttca gcgcgcccgc gaccagcagc ccgttgagcg tgaggctttc
     1621 ggcgccggtg cccccggtaa cggcgatgtc gccaagcagt accgggcgct tgcggttggc
     1681 cgcctggatc accagttcgg ccccggccat cgggatggtc agatcttcct cgtaggtgcg
     1741 gttatcctcg atctggatga ccgtgcgcgg ggcggtcgcc gggtcccagg cggccagcgc
     1801 gtcggccagc gtgttgatgc cggtcgaggg cacgcggatc agggcgtcga gcgcggcggg
     1861 cgcggcaacg gtgtcgggtt cggtgggcgc gtcctcaccg tgggcagagc ccagcgcggg
     1921 cggacgacgc cggtcgtagg ggccgccccc gatgtcggcg ctgaagccgt agttgtaact
     1981 gacctggacg gtgttcggcg tctcgccggt ggcgaaggcc agccgcccgc gccgcacgtc
     2041 cacgcccacg cgccccgccg ggggccggtc ccagttgcgc aggtccatgc acatgatcgc
     2101 gatgggcggc accggctcac catcggcgat gatgtccagg ctgccgttgg ggccgtagta
     2161 gatgctgtcc gccgggcgat ctgcgggcgg cacacttgca tagcgggcct ggtagctttg
     2221 caggtcggcg aagaaagcgg cctggcggat cggctggggc acgttggtct cgtcggccag
     2281 ttgggtgatc tcgcgctcgg tctgcgggcg agtgaacagc gcggcgtcgt tgcccagcgg
     2341 gctgaaccag tagc
//
LOCUS       DASUYC010000924         3174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1540773, whole genome shotgun sequence.
ACCESSION   DASUYC010000924 DASUYC010000000
VERSION     DASUYC010000924.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1540773"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VF276_15115"
     CDS             complement(<1..291)
                     /locus_tag="VF276_15115"
                     /inference="COORDINATES: protein motif:HMM:NF017221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX6608231.1"
                     /translation="MCPMPTGQMSPAEFQERLNALTQELRQEQEALNRENTEVDLLLR
                     QVQGEVDKLGQREAAIKSRVRDMELNLDNYTRQDMKTIYNNAHEHDMRLFMMR"
     gene            complement(383..>3174)
                     /locus_tag="VF276_15120"
     CDS             complement(383..>3174)
                     /locus_tag="VF276_15120"
                     /inference="COORDINATES: protein motif:HMM:NF016611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6608232.1"
                     /translation="LLISWVLLNVPQPKPPSGQFVLAIAQFGELQPDGQGWQIVPGQR
                     STWDQARAVLPANLPLPSHWPALRLRLNPAPPSANGASPAHAVSRGAGGAGGAMAPAP
                     HGSIIAPIHRPPLTAGGATITTLARPAAALPAEALEDGLAERPPPPPRPRPLPTRAAA
                     ATEWFYTTLLARWTDTGVSGIVPTVTGFAPDSATARALAASVKADAALWGVVRGGSDP
                     AVWTYVELLTNPEQRAQAEDAELRLAGLTAFPLGPISPTRPELVLSMLTGLAYYASRL
                     YRQALNEWNLALIAALVAHDMPPETPLSAVAVAHFYRGNALFLLRDLPAAALAYEAAI
                     TAAPRLAEAQHNLGLVYHALGRLDRAHERISEALKLNPKLELGRYNLGVVLLSQGQLE
                     PAKQVFRDLLARDPNNAAVHRALGLVHRGAGQPEAAEAELQEALRLRPDYADVYSDLA
                     ALYNQRAVACAGAGETGARGDWMARASAALLRSIELRPANPEARYNYGVMLNADGQVD
                     AAADEFKEALRLRPDFPEAHYALGVLYKSQGRLDQAVAQFKEATALKPANAETFINLG
                     LAEYGEQHYAEAAEKFRQALSLEANNTEAHFRLGMALARMNDAEAIGHLQQALAAPNP
                     PPEAAHELAAIYRRQNNPADAIRILQQQIGRDRHDAQAYYALGTIYVHQKRIDEAIEA
                     FRVAVQYAPDLAPAHHNLGVAYVAKGRLDEALAEFERMAELAPDDPQAHYVLGVAYKD
                     RGNYERAADELNTAIRLNPDFTEAHYNLGLVYQALGKPEPAISEFRDALRLQPTHGRA
                     LYRLGTAYAAAGELDASIGVFQRLVDAQPQMAEAHYNLGVAYQAQHNWAGAVREFVAV
                     VRLRPDDAEAFNSLGLAYKNAGRVEEAINAYRRAVQLKPEYAQAYYNLGQAYTAINEI
                     NLAVQSFRTYEDLAK"
BASE COUNT          406 a         1031 c         1189 g          548 t
ORIGIN      
        1 gcgcatcatg aacaggcgca tgtcatgttc gtgcgcgttg ttgtagatcg tcttcatgtc
       61 ctggcgcgtg tagttgtcca ggttcaactc catgtcgcgc acgcggctct tgatcgcggc
      121 ttcgcgctgg cccagtttgt cgacctcacc ctgcacctgg cgcagcagca gatcgacctc
      181 ggtgttctcg cggttcaacg cctcctgttc ctggcgcaac tcctgggtca gtgcgttcag
      241 gcgttcctgg aactcggccg ggctcatctg gccggtaggc atggggcaca ttgcgggttc
      301 tccctctcta gatcgtgacg gggtgtggtc gccggtacgg tgttattata tggtacaggc
      361 cgcgcccggc gcaatagcgg gcctactttg ccaggtcttc ataggtgcgg aaggactgga
      421 cggccaggtt gatctcgttg atcgcggtgt acgcctgacc caggttgtaa tacgcctggg
      481 catattccgg cttgagttgg acggcgcggc ggtaggcgtt gatcgcctcc tcgacgcgcc
      541 cggcgttctt gtaggccagg cccaggctgt tgaacgcctc ggcgtcgtcg gggcgcaggc
      601 ggaccacggc gacgaactcg cgcacggcgc cggcccagtt gtgctgggcc tgataggcca
      661 cgcccaggtt gtagtgagcc tcggccattt gcggctgcgc gtccaccagc cgctggaaca
      721 cgccgatgga ggcgtcgagt tcgccggcgg cagcgtaggc cgtgcccagg cggtagagcg
      781 cgcgcccgtg cgtgggttgc aggcgcagcg cgtcgcggaa ctcggagatg gcgggttcgg
      841 gcttgcccag cgcctggtaa accaggccca ggttgtagtg cgcctcggtg aaatcggggt
      901 tgagccggat cgccgtgttc agttcgtccg cggcgcgttc gtagttgccg cggtctttgt
      961 aggccacacc cagcacatag tgcgcctgcg ggtcgtcggg cgccagttcg gccatgcgct
     1021 cgaattcggc cagggcctcg tccagccggc cctttgccac ataggcgacg cccaggttgt
     1081 ggtgggcggg cgccaggtcg ggcgcgtact gcaccgcgac ccggaaggct tcgatggcct
     1141 cgtcgatccg cttctggtgc acatagatcg tgccgagcgc gtagtacgcc tgggcatcgt
     1201 gccggtcgcg cccgatctgc tgctggagga tgcggatggc gtcggcgggg ttgttctggc
     1261 gccggtagat ggcggccagt tcatgcgccg cttccggcgg ggggttgggg gcggccagcg
     1321 cctgttgcag gtggccgatg gcctccgcgt cgttcatacg cgccagggcc atgcccagcc
     1381 ggaaatgggc ctcagtgttg ttggcctcca ggctcagcgc ctggcggaac ttctccgcgg
     1441 cttcggcgta gtgctgttcg ccgtactcgg ccaggcccag gttgatgaag gtctcggcgt
     1501 tggccggttt gagcgccgtc gcctccttga actgggccac ggcctggtcg aggcggccct
     1561 ggctcttgta gagcacgccc agggcatagt gcgcctcggg gaaatcgggg cgcaggcgca
     1621 aggcctcctt gaactcgtcg gcggcggcgt ctacctggcc gtcggcgttc agcatcacgc
     1681 cgtaattgta gcgggcctcc gggttggcgg gccggagttc gatggaccgc agcagggccg
     1741 cgctcgcccg cgccatccag tcgccgcgcg ccccggtttc gcccgcgccg gcgcaggcca
     1801 cggcgcgctg attgtagagc gcggccaggt cgctgtagac atcggcgtaa tcggggcgca
     1861 gccgcagcgc ttcctgtagc tcggcttcgg cggcttccgg ctgcccggct ccgcgatgca
     1921 ccagccccag tgcccggtgc actgcggcgt tgttcgggtc gcgggccagc aggtcgcgga
     1981 agacctgctt cgccggttcg agttgcccct ggctcagcag gaccacgccc aggttatagc
     2041 ggcccaactc cagcttgggg ttcagcttca acgcctcgga aattcgctcg tgcgcgcgat
     2101 ccagccggcc cagcgcgtgg tagaccaggc ccaggttgtg ctgggcctcg gccaggcgcg
     2161 gcgccgcggt gatcgccgct tcataggcca gggcggcggc gggcaggtcg cgcagcagga
     2221 agagggcgtt gccccggtag aaatgggcca cggccaccgc gctgagcggc gtctccggcg
     2281 gcatatcatg ggcgaccagc gcggcgatca gggccaggtt ccactcgttg agcgcctggc
     2341 gatagagccg cgaggcgtag taggccagcc cggtgagcat gctcagcacg agttcgggcc
     2401 gggtgggcga gatcggcccc agcgggaagg ccgtgaggcc ggccagccgc aattccgcgt
     2461 cttcggcctg cgcgcgttgc tcgggattgg tcagcagttc gacgtaggtc cagaccgccg
     2521 ggtcgctgcc gccgcgcacc acgccccaga gcgcggcgtc ggctttcacg ctcgcggcca
     2581 gggcgcgggc cgtggccgaa tcgggggcga agccggtgac ggtgggcacg atgccgctga
     2641 cgccggtatc ggtccagcgg gccagcagcg tggtgtagaa ccactcggtg gccgcggcgg
     2701 cgcgggtggg cagggggcgc gggcggggcg gcggcggggg tcgctcagcc aggccgtctt
     2761 cgagcgcttc cgcgggcagc gcggcggcgg ggcgggccag cgtggtgatc gtcgcgccgc
     2821 ccgccgtcag cgggggccgg tggatcgggg cgatgatgct gccgtgcggc gcgggggcca
     2881 tggcaccgcc ggcgccgccc gccccgcgcg agactgcgtg cgcgggactc gcgccgttcg
     2941 ccgaaggggg cgcggggttc aggcgcaggc gcagcgccgg ccagtggctg ggcagcggca
     3001 agttggccgg cagcacggcg cgcgcctggt cccacgtcga gcgctgcccc ggcacaatct
     3061 gccagccctg gccgtccggc tgcaattcgc cgaactgggc aatcgccagc acgaactgcc
     3121 cgctgggcgg cttcggttgg ggcacgttca gcagcaccca actgatcagc aata
//
LOCUS       DASUYC010000925         3029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1283070, whole genome shotgun sequence.
ACCESSION   DASUYC010000925 DASUYC010000000
VERSION     DASUYC010000925.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1283070"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VF276_15125"
     CDS             <1..364
                     /locus_tag="VF276_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX6608233.1"
                     /translation="KPPAQLVPATLGSPGAMRVGDEAYVVGNPFGLYSSMSAGVISGF
                     GRAFQPVGSQYKLQDLIQIDAAVNPGNSGGPLLNRYGEVIGIVTGLVNPTAQNFFVGV
                     GFAVPITTAGGAAGAPQY"
     gene            391..1401
                     /locus_tag="VF276_15130"
     CDS             391..1401
                     /locus_tag="VF276_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX6608234.1"
                     /translation="MDQRTNGKPGLPMERVLYEVKKVIVGQDHLLESLVVALLARGHI
                     LVEGVPGLAKTMAIKTLAEAIGGEFKRIQFTPDLVPADLVGTRIYNQKTGEFNTSLGP
                     VFTNLLLADEINRAPAKVQSALLEVMQEHQVTIGTETFRVPDPFLVLATQNPIETEGT
                     YALPEAQVDRFMLKVLVGYPSPNEEFVIVERMTGFLEEVQKVLTTAQLLELQRAADRV
                     YVDPALIQYAIHVVSATRSPKAIGLKELQPYIMFGASPRASINIVRAARALAFVRGRT
                     YALPQDALDMVRDVLRHRIVLSYEALSDNLSSDDLLTKILDRIPVPQVPLHEYADRRA
                     HA"
     gene            1370..2269
                     /locus_tag="VF276_15135"
     CDS             1370..2269
                     /locus_tag="VF276_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX6608235.1"
                     /translation="MNTPTAARTPERVLQRLDWQVIRRLDGLLQGDYRTLFYGYGVDF
                     ADLREYQPGDDVRYIDWNVTARLTTPYVRQYAEDREVTAWFLLDLSPSMDFGSVQSLK
                     RSVLIDCVATLARLLTRHGNRVGAIFYGNGVERTIPAGSGRLHVLRLINDLLRQPPLP
                     KAPFTDLTPLVQAGLHTIRRRTLIFIVSDFISAPGWDRALRLLNQRHEVIAIRLWDPR
                     EMELPDAGPLILEDAETGEQLYVDTRDKAFRKRFQEAAQQREAALQETFKQAGVDTLA
                     LSTDEDLVRAIVRFATLRRQRRH"
     gene            2382..>3029
                     /locus_tag="VF276_15140"
     CDS             2382..>3029
                     /locus_tag="VF276_15140"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX6608236.1"
                     /translation="MSFIWPAMLVLLLLIPPFVVVYLRLQQRRRQLVAGYANPGLVLD
                     RGRARTGRRRHIPALFFLLALIILVVALARPQALVSLPRIEGTVLLAFDVSGSMAADD
                     MKPTRMDAAKAAARAFVEHQPSTVQIGVVSFSDSGFAVQAPTNDPGPVAAAINRLAPQ
                     RGTSLARGIQAALNAIALGSSGPLTLANPRSEPTPTPTAVPKGSHTSAVIVLLTDG"
BASE COUNT          485 a         1079 c          987 g          478 t
ORIGIN      
        1 caagcccccg gcccaactcg tcccggccac gctcggcagt cccggcgcca tgcgcgtggg
       61 cgacgaggcc tatgtcgtcg gcaacccctt cggcctctat agctcgatga gcgcgggcgt
      121 catttcgggc ttcgggcggg ccttccagcc ggtgggcagc cagtacaaac tgcaagactt
      181 gatccagatc gacgcggcgg tcaatcccgg caattcgggc ggcccgctgc tcaatcgcta
      241 cggcgaggtc atcggcatcg tcaccgggct ggtcaacccg acagcccaga acttcttcgt
      301 gggggtgggc ttcgccgtgc ccatcaccac cgcgggcggc gcggcgggcg cgccgcaata
      361 ttagacgcca gactatcgaa ggagacgcgc atggatcagc ggacgaacgg caaacccggc
      421 ttgccgatgg agcgggtgct ctacgaagtc aagaaggtaa tcgtcggcca ggatcacctg
      481 ctggagtcgc tggtcgtcgc gctgctggcg cgcgggcata tcctcgtcga aggcgtgccg
      541 gggctggcga agacgatggc gatcaagacc ctggccgagg ccatcggcgg cgagttcaag
      601 cgcatccagt tcacccccga cctggtgccc gccgacctgg tcggcacgcg catctacaac
      661 cagaagacgg gcgagttcaa tacctcgctc ggcccggtct tcaccaacct gctgctggcc
      721 gacgagatca accgcgcccc ggccaaggtg caaagcgccc tgctcgaagt gatgcaggag
      781 caccaggtga ccatcggcac cgagaccttc cgggtgcccg accccttcct ggtgctggcg
      841 acgcagaacc ccatcgagac cgagggcacc tacgccctac ccgaagccca ggtcgaccgc
      901 ttcatgctca aggtgctggt cggctacccc agccccaacg aggaattcgt catcgtcgag
      961 cgcatgaccg gcttcctcga agaggtgcag aaggtgctga ccaccgcgca gttgctggag
     1021 ttgcagcggg ccgccgaccg tgtttacgtg gacccggccc tgatccagta cgccatccac
     1081 gtggtgagcg ccacccggtc gcccaaggcg atcggcttga aggagttgca gccctatatc
     1141 atgttcggag ccagcccgcg cgcctcgatc aatatcgtgc gcgcggcgcg ggcgctggcc
     1201 ttcgtgcgcg ggcgcaccta tgccctgccg caagacgcgc tggatatggt ccgcgacgtg
     1261 ctgcgccatc gcatcgtgct ctcgtatgag gcgctgtccg acaacctgag cagcgacgac
     1321 ctgttgacca agattctcga ccgcatcccc gtgccgcagg tgcctttgca tgaatacgcc
     1381 gaccgccgcg cgcacgcctg agcgcgtcct gcaacgcctc gactggcagg ttatccgccg
     1441 gttggacggc ctgctgcaag gcgactaccg cacactgttt tacggctacg gggtcgactt
     1501 cgccgacctg cgcgaatacc agccgggcga cgacgtgcgc tatatcgact ggaacgtgac
     1561 cgcgcggctg accacgccct acgtgcgcca gtatgccgag gaccgcgaag tcaccgcctg
     1621 gttcctgctc gacctcagcc cctcgatgga cttcggcagc gtgcagagcc tgaagcggtc
     1681 ggtgctgatc gactgcgtgg cgaccctggc gcggctgctc acgcgccacg gcaaccgcgt
     1741 cggcgccatc ttctacggca acggcgtcga gcggacgatc ccggcgggca gcgggcggct
     1801 ccatgtgctg cgcctgatca acgacctgct ccggcagccg ccgttgccca aggctccgtt
     1861 caccgacctg acgccgctgg tccaggccgg gctgcacacc atccggcggc gcacgctgat
     1921 cttcatcgtc tcggatttca tcagcgcgcc gggctgggac cgggccctcc gtttgctgaa
     1981 tcagcggcac gaggtcatcg ccatccgcct gtgggacccg cgcgaaatgg agctacccga
     2041 cgcggggccg ctcatcctgg aggatgccga gacgggcgag cagttgtatg tggacacccg
     2101 cgacaaggcg ttccgcaagc ggttccagga ggcggcgcag cagcgcgaag ccgccttgca
     2161 ggagacgttc aagcaggccg gggtcgatac cctggccctc tcgaccgacg aggacctggt
     2221 ccgcgccatc gtgcgcttcg ccactttacg gcggcaacgc cgccactagg cggtgggccc
     2281 tgctcggcgc aatcatggca cctgtagggg ccggttgcca accggctcgc taacgagcca
     2341 cctgggacgc ctggggcggt cccgcggaaa tggacgaacc tatgtcattc atctggcccg
     2401 cgatgctggt cttgctgctg ctgatcccac cgttcgtggt ggtctatctc cgcttgcagc
     2461 agcggcggcg gcagttggtc gcggggtacg ccaatccggg cctcgtgctc gatcgcgggc
     2521 gcgcccgcac gggccgccgc cgccacatcc cggcgctgtt cttcctgctg gccctgatca
     2581 tcctggtcgt ggcgctggcc cggccccagg cgctggtcag cctgccgcgc atcgagggca
     2641 cggtgctgct ggccttcgac gtatcgggca gcatggccgc cgacgacatg aagccgaccc
     2701 gcatggacgc ggcgaaagcg gcggcccgcg ccttcgtgga gcaccagccg tccaccgtgc
     2761 agatcggcgt ggtctcgttc agcgattccg gctttgccgt gcaggcgccg accaacgacc
     2821 ccggccccgt cgccgccgcg atcaaccggc tggcgccgca gcgtggcacg tcgctggcgc
     2881 gcggcatcca ggcggcgctc aacgccatcg ccctgggcag cagcggcccg ctgacgctcg
     2941 ccaacccgcg cagcgagccg acgcccaccc cgacggcggt gccgaaaggc agccacacgt
     3001 ccgccgtgat cgtgctgctc accgacggc
//
LOCUS       DASUYC010000926         2034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1541127, whole genome shotgun sequence.
ACCESSION   DASUYC010000926 DASUYC010000000
VERSION     DASUYC010000926.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1541127"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..249)
                     /locus_tag="VF276_15145"
     CDS             complement(19..249)
                     /locus_tag="VF276_15145"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEX6608237.1"
                     /translation="MFTVSTHSGERWHTWLGEARFTHQTPRTLARFQSVPDSYQLPDK
                     AALACRVLGNGVPCLVAQRVTESLLDAPEHAA"
     gene            complement(509..985)
                     /locus_tag="VF276_15150"
     CDS             complement(509..985)
                     /locus_tag="VF276_15150"
                     /inference="COORDINATES: protein motif:HMM:NF024784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX6608238.1"
                     /translation="MPNSCALCGAPLSQRNRTGYCPRRCYDAARRSSSAPCLGCGGPL
                     GQSNQSGYCRRCTMRNAQRARNQRGAANPGWKGGKHVDERGYIRVRIDGKHRREHRVI
                     AEQVLGRSLTAREVVHHKDGNTGNNDPANLEVYCCNAHHLWHNWQGDHPIQVSPLS"
     gene            complement(1009..1509)
                     /locus_tag="VF276_15155"
                     /pseudo
     CDS             complement(1009..1509)
                     /locus_tag="VF276_15155"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
     gene            complement(1437..1889)
                     /locus_tag="VF276_15160"
     CDS             complement(1437..1889)
                     /locus_tag="VF276_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608239.1"
                     /translation="MNNAVFAARVRALVPAPVCGHPAARVRQGLCPPCYARAWRAEQR
                     ATRADLAEAGWSVADVAGAFGLPIIVVLWWIWNGALAAQRAGERYAVGDGALHAFLWR
                     YPAAWAQAPLRDRAWVEGLLHDPDLCKSATEPADVRARRQAAERGRAA"
BASE COUNT          321 a          715 c          659 g          339 t
ORIGIN      
        1 cggcctccgt gcctgcgctc atgccgcgtg ctccggggcg tcgagcaggc tctcggtcac
       61 gcgctgggcc accaggcagg gcaccccatt gcccagcacc cgacaggcta acgccgcctt
      121 gtccggtagc tgatagctat ccggcacgct ctggaaccgg gccagcgtcc ggggcgtttg
      181 atgtgtaaac cgggcctcgc ctagccaggt atgccaacgt tcgccgctat gcgtactgac
      241 ggtgaacatc ggctgctcgg cccagcgcac accgtccccg tacttatcgg cattccggtt
      301 taggatatag ggccgcgacg ggcgataggg ccggtatgct tcctgccagg gcgcaaactg
      361 ggacgccggt agcgtcggca gtaaatcttc gattgccgcg taccacccca cccacggcac
      421 gggcgcgggc aaggggcgga acggctgcca cagcccgccg ctcacagcgc gcaagatcag
      481 ccgccgccgg gtctggggga ccgctgtttc acgacagagg ggacacctgg attgggtgat
      541 ctccctgcca gttgtgccag aggtgatggg cgttgcagca atagacttca agattagccg
      601 gatcgttatt gcctgtattt ccgtctttgt ggtggaccac ctcgcgcgca gtgagggatc
      661 gtccaagcac ttgctcagca atgacacggt gttcccgtcg atgcttcccg tcaatccgga
      721 cgcggatata gccgcgctcg tcaacatgtt tcccgccctt ccatccaggg ttagcggctc
      781 ctcgttgatt tcgtgcccgc tgtgcgttcc gcattgtgca gcgccggcag taccctgatt
      841 gattgctttg gcccaatgga ccgccacatc ccaggcaagg tgccgagctt gatcgtcggg
      901 ctgcatcata gcagcggcgt gggcaatatc cggtgcgatt acgttgactg aggggtgccc
      961 cgcagagggc gcatgaattg ggcatgggat aaaacctcca taatccgcgg cgttcaccac
     1021 cgccgcgtgc gaccagtagt tgaggctatc cagcgccgcc cggatcgccc ggtaggacgc
     1081 gctcccggcg tagccttcca cgttctccag ccagacgatc cgcgggcgga tcgtcgtgat
     1141 ggcgcgtaca atcgcagcgg caatcgcgcg gtcctgctct gtttcctgcc cgcctgcctt
     1201 cgccacggag aaggacgggc acggcggcga tacctgtacc acatcgacgc cacgccacgg
     1261 gcggtagtcc accgcttgca cgggcgcgac ggtgcaatga tcgcccaggt tcgccgtgta
     1321 tgcggcggca atggcggggt catactctat cgccccgaca agctgaatcc ccgcctgctg
     1381 cattcccagc gcggccaggc cgccgccgct gcacagcgtg atgccggtta gggtagtcat
     1441 gccgcccgcc ccctttccgc cgcttggcgg cgcgcccgca cgtccgccgg ctcggttgca
     1501 ctcttgcata agtccgggtc gtgcaatagc ccctccaccc acgcccggtc ccggagcggc
     1561 gcctgtgccc acgcggcggg atagcgccac aggaaggcgt gcaaggcgcc gtcgcccacg
     1621 gcgtagcgct ccccggcccg ctgcgcggcc agggcgccgt tccagatcca ccacagcacg
     1681 acgatgatcg gcaggccgaa cgccccggcg acatcggcca cggaccagcc cgcctcggcc
     1741 agatccgccc gcgtggcgcg ctgctcggcc cgccaggccc gcgcgtagca cggggggcac
     1801 agcccttgcc ggacccgcgc ggcgggatgg ccgcagaccg gggcaggaac cagcgcacgg
     1861 acgcgggcgg cgaagacggc gttattcatg ggtagcctca tagcgcccca gccaccacgc
     1921 ccgaatctgc gcggccagcg ccgggtcggc tttggcggcg gcgatgtcgc tatcgcaagc
     1981 ggccacgccc gcctgcaaat ccagccgcgc ggcggggtcg tgttcgtccc gcag
//
LOCUS       DASUYC010000927         2661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1541345, whole genome shotgun sequence.
ACCESSION   DASUYC010000927 DASUYC010000000
VERSION     DASUYC010000927.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1541345"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VF276_15165"
     CDS             complement(<1..346)
                     /locus_tag="VF276_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608240.1"
                     /translation="MIPPTTQSPPRRPTFRRPYEALLRPVPAVERPPLALPLWVHLLV
                     LAGIAALAFDIGRGMWGWSYDDAFIIYRYARNIVEGHGMVYNPGEYYLGTTSAGYTLL
                     LAGLHFVLPWLDF"
     gene            complement(497..1720)
                     /locus_tag="VF276_15170"
     CDS             complement(497..1720)
                     /locus_tag="VF276_15170"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX6608241.1"
                     /translation="MAARPTLAALTGLRFFAALHVVLFHFAAPVLASAPEWARNFAAA
                     GLTGVDLFFILSGFVLAYNYLDGRQMRRREFWVARFARIYPAHLVGVLLLAPYIIYAY
                     FTVFPAPAAAGKTLGDAGLALTLTQAWVPGSWSAWNGPAWSLGAEAFFYALFPFLGLA
                     LGRIPVRLLPVALGGFWVLAVLRPASYILYDIPGWWPVAYANPILRLPEFLMGIVLGR
                     LFLHYGRARWGGWLAALGVCGWVGLLGIGVPGVNSLLARTVLARLNSALLYEALLLVF
                     FVLLIYGLAHGYGVVAKGLSLPFMLLLGEASYGLYILQVPVAAAVKGLTEQRMDPVML
                     PYIKYFETVPYFLAYVAILVMLAIISHQVVEAPARTFLKRALNRPRPARVPAVSEPAL
                     PAPVVPPAIEAPEIP"
     gene            complement(1896..>2661)
                     /locus_tag="VF276_15175"
     CDS             complement(1896..>2661)
                     /locus_tag="VF276_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608242.1"
                     /translation="KRRILFAVLYGLGLLTKLSLAPLLLPAVAVFWVRCTGSPTQRIR
                     ALLPAAGVTVLLAGPFYLYRWLAYGDPLAGAAWQAMIPTDSPWHLTDLFWLVDPFRAN
                     LWNSFWGNYGWQRVPLPDAFYLAFAILTGLAVLGGIILLARRALTPVQRAGCAVFLSV
                     LLLEYAVVIVISLRLIAWQGRELFPALSSVSVLFGFGLAGLVLGPAAIRSVGGPEPKL
                     PVVGVGVLLILLAGLLAANIYSIVWVVAPVLNSSGL"
BASE COUNT          624 a          882 c          838 g          317 t
ORIGIN      
        1 ggaaatcgag ccagggcaac acgaaatgca gcccggccag cagcagggta tagccggcgg
       61 acgtggtgcc caggtaatac tcgccgggat tatagaccat gccgtggcct tcgacgatgt
      121 tgcgggcata gcgatagatg atgaaggcgt cgtcgtagct ccagccccac atcccgcggc
      181 caatatcaaa ggccagcgcc gcgatccccg ccagcaccag caaatgtacc cacagcggca
      241 gcgccagcgg cggacgctcg actgcgggca ccgggcgcag cagcgcctca taggggcgcc
      301 gaaaagtggg gcgccgggga ggggattggg tagtgggcgg gatcaattcc attactccat
      361 ttcgccgaac cagcgatctt cgactagatc aatgcgtaaa tcccggcgcg gcggcacgct
      421 tatccggcgc gtgacacaac gcgggccata accaacagag tataacaaag gttgaccggg
      481 atttgcaaag ccgggatcag gggatctccg gcgcttcgat ggcgggcggc acgaccggcg
      541 cgggcagggc cggctcgctg acggcgggca cgcgggccgg gcggggccgg ttcagcgcgc
      601 gcttgaggaa ggtgcgggcg ggcgcttcca cgacttgatg gctgataatc gccagcatca
      661 ccaggatcgc cacgtaggcc aggaaatagg gcaccgtctc gaagtacttg atgtagggca
      721 gcatgaccgg gtccatgcgc tgctcggtca gccccttcac cgccgcggcc acgggcacct
      781 ggagaatata gaggccgtaa ctggcctcgc ccagcagcag cataaagggc agggacagcc
      841 ctttcgccac cacgccgtag ccatgcgcca ggccgtaaat cagcaggaca aagaagacca
      901 gcaacagcgc ctcgtacagc agcgcgctgt tcaaccgcgc cagcacggtg cgcgccagca
      961 gactgttgac gcccggcacc ccgataccga gcaaccccac ccagccgcac acgcccagcg
     1021 cggccagcca gccaccccag cgggcgcggc catagtgcag aaagagccgc cccagcacga
     1081 tgcccatcag gaactcgggc aggcgcagga tggggttggc gtaggcgacg ggccaccagc
     1141 cggggatgtc gtataggata taggaggccg ggcgcagcac cgccagcacc cagaacccgc
     1201 ccagggccac cggcagcagc cgcacgggaa tccggcccag ggccaggccc aggaaaggaa
     1261 agagcgcgta gaagaaggct tcggcgccca gcgaccaggc cgggccgttc caggccgacc
     1321 acgagccggg cacccaggcc tgggtcagcg tgagcgccag cccggcatcg cccagcgtct
     1381 tcccggcagc ggcgggcgcc gggaacacgg tgaaataggc atagatgata tagggcgcga
     1441 gcagcaggac acccaccagg tgggccgggt aaatgcgcgc gaagcgggcc acccagaact
     1501 cgcgccgccg catctgccgc ccatcgagat agttgtaagc cagcacaaac cccgacagga
     1561 taaagaacag gtcgacgccc gtcagccccg cggcggcaaa gttgcgcgcc cattccgggg
     1621 cggatgccag caccggcgcg gcgaagtgaa acaggaccac atgcagcgcg gcgaaaaagc
     1681 gcagtccggt cagcgccgca agggtcggtc gcgcagccat cagcgactcc gcgcccggcc
     1741 cgcggcccgg cgcacagacc agggacgcgc ccggtctatc gatattgatg ccggtaccgg
     1801 caggggaaga ttatgcacgt tctagatgat accaaaaagc cggcccgctc acaactgccc
     1861 tgccgggaac cggcgccgcc ctagactctc gccgatcaga gtccactgga gttcagtaca
     1921 ggagccacca cccagacgat actatagatg ttggcagcca acagccccgc aagcagaatg
     1981 agtagcacgc ccaccccaac cacaggtaac ttcggctccg gcccgcccac agaccggatc
     2041 gctgccggcc ccaggaccag accggctagt ccaaagccaa agagcacact gacgctcgac
     2101 aacgccggga acagttcgcg cccctgccag gcaatcagac gcaggctgat tacgatcacc
     2161 acggcgtact ccagtagcaa gacgctcagg aatactgcgc accctgcgcg ttgaaccggc
     2221 gtcaatgccc gccgggcaag caagatgatg ccgcccagta ccgctaagcc ggtcagaatc
     2281 gcaaacgcca gataaaaggc gtccggcaac ggcacccgtt gccagccata gttgccccaa
     2341 aatgaattcc aaagattggc gcgaaaaggg tccactaacc agaacaaatc agtgagatgc
     2401 cagggactgt ccgtcggtat catggcctgc caggccgcgc cggcaagggg atcgccatag
     2461 gctagccagc ggtaaaggta aaacggcccc gccaagagca cggtcacacc tgccgcagga
     2521 agtaaggcac gaatacgctg agtcggagac cccgtacaac gcacccaaaa taccgctacc
     2581 gcgggcaaaa gcagaggcgc caggctcaat ttcgttaaca acccgaggcc gtaaagcacg
     2641 gcaaacagga tgcgtcgttt c
//
LOCUS       DASUYC010000928         3392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_616520,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000928 DASUYC010000000
VERSION     DASUYC010000928.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616520"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(499..672)
                     /locus_tag="VF276_15180"
     CDS             complement(499..672)
                     /locus_tag="VF276_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608243.1"
                     /translation="MKTVSFKLEPNQPAYLEVRIDPAAHGEAGLGPIERGVAIKTAAN
                     QTLEFRLTAQVVK"
     gene            complement(745..1062)
                     /locus_tag="VF276_15185"
     CDS             complement(745..1062)
                     /locus_tag="VF276_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608244.1"
                     /translation="MSTKTKRRGPAAAAALVRPAQVRPRPVPRRQAQRRIPWMPVVIG
                     LVLVGALAVLLWGAASMPRGTVAAATTTHDFGQVPISGGDLTTQFPLTVDRAALVTDI
                     TST"
     gene            complement(1291..1464)
                     /locus_tag="VF276_15190"
     CDS             complement(1291..1464)
                     /locus_tag="VF276_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HEX6608245.1"
                     /translation="MVTDPVCGMEIDAKDAAGTSEYQGQTYYFCSPGCKKQFDKNPQQ
                     YVGQQGDQQHPQH"
     gene            complement(1565..2971)
                     /locus_tag="VF276_15195"
     CDS             complement(1565..2971)
                     /locus_tag="VF276_15195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HEX6608246.1"
                     /translation="MEMPILWSLIHDLEQLRKRDRWTREQLQTFQEQALSRMRQYAYA
                     RSPFYQQFHAGLMDRPLQDLPVLTKSMLMEHFDDLVTDRAVHLEAVRQYVAGAREAER
                     FLGRYWVNATSGSSGRPGIFLLDRTEWAAVLASFARAQEWAGAQVRLTQQTKLAAVAS
                     TDPRHMSAAVGATVRSWWMPTLRLAASDPLPDIVAQLNAWQPDLLVSYASMARILADE
                     QLAGRLRITPHLVFTSSEVLTDETRRLVAAAWGQEPFNQYAATESGGLAAECVAHRGM
                     HLFEDLVIPEVVDEHNRPVAPGTFGAKLLITVLASRTLPLIRYELSDSLRLSTATCPC
                     GRHYTLIDAVQGRREDILHFPSAAGADVAVQPHTFHRILDQTPVSGWRVVQEADGGLT
                     ILLSGISAGAIEEALAQQVRVALAAQRVSVPYVHVMQVDTIPKGASGKAPLIQARPAR
                     TASPAASAAPAGNALLGR"
BASE COUNT          571 a         1095 c         1112 g          614 t
ORIGIN      
        1 gcctcgtact cctgggccat gtgcgcggcc gtcgtctgca gtgtgtaatc cagggcgtca
       61 tagtggccgc gagtgtgggc ggcatagagg aacaggccga ccagcaacac caccaggccg
      121 gtcagcccgc cataccacag cccgaggcgc aggcgcagcg acatcggcta cgccttccac
      181 cataccgagc cgtggtcccg gcgagagggg atccggcggc gcctacccgc gaatgcacgt
      241 cgccataccc gttgtacacg gaatgccata acaccgcgtt gcttgaccgg gacgtgcgct
      301 tgcggcttgt agcgcggcgt acccatggcc aagccccgag aagtaggttt ctgcttcaac
      361 atgctgatcc gcacaggctt atcgagtagc aggatgcggt gcatcgctgt ctgctgtaca
      421 ctgctcttct tccagccggc gcggcccatc ggccgcaccg gcatcggacc ggatcccccc
      481 gtttctattc tagcagatct atttcacgac ttgtgccgtc aggcgaaact ccaaggtctg
      541 gttggcagcg gtcttgatcg ccacaccgcg ttcgatgggg ccgaggcccg cttcgccgtg
      601 agcggcggga tcgattctta cctccaagta ggccggctga ttcggctcca gcttaaagct
      661 caccgtcttc acgggaccat cgtgttgcat gccgacccag tcggatgccc tgtccgcctg
      721 cacgaggcgg ccggtggtgc acattcaggt gctggtgatg tcggtgacga gggccgccct
      781 atcgacagtg agggggaact gggtcgtgag gtcgccgcca ctgatgggca cctgcccaaa
      841 gtcgtgggtc gtggtcgccg cagccaccgt gccgcggggc atgctcgcgg caccccacag
      901 gagtaccgcc agcgccccaa ccaggaccag gccaatgacg accggcatcc agggtatgcg
      961 ccgctgcgcc tgacggcgcg ggacaggccg cggtcgcacc tgggcaggtc ggaccagcgc
     1021 cgccgccgcg gccgggccgc ggcgtttagt ttttgtgctc atctgcttct ctcctctcgc
     1081 agctttacag gaagctggtt gtttcggagt aagggtaccg cccttcgctg aattgctgct
     1141 gaagacaagc tgaatgctgg ctgaggaaat aggaggagtg attcagtcgc gggcgcgctg
     1201 tgccgggggc agatccggcc acggcgacgt acgccgcctc gtagcccgag acggcgtacc
     1261 tctgttattc aagggcgcta cacggcgcct ttagtgctgc gggtgctgtt gatcgccctg
     1321 ctggcccaca tactgctgcg ggtttttgtc gaactgcttc ttgcagccag gggagcagaa
     1381 atagtaggtc tgcccctgat actcgctggt gcccgccgca tccttggcgt cgatctccat
     1441 accacatacc gggtccgtga ccatgacctt cacccccttc gtatataacg cgatgacccg
     1501 gcatctgtac gccgggaata caggcatgcc cagactcccg cctgcccaat tgaccctgcg
     1561 tggcttatct tccgagcagc gcgttgcctg cgggagcagc gctcgcggca gggctcgccg
     1621 tccgggccgg gcgcgcttgg atcaggggtg ctttgccgga cgcgcccttg ggaatcgtgt
     1681 ccacctgcat tacatgcaca tagggcacgg acacacgctg cgccgccagg gccacgcgaa
     1741 cctgttgcgc aagcgcttcc tcaatggcgc cggcggatat gccactaagc aagatggtca
     1801 gtcccccgtc ggcctcttgc acgactcgcc agccgcttac gggcgtttgg tctaagatgc
     1861 ggtggaaagt atgcggctgc acggccacgt ctgcacctgc cgcactcgga aagtgcagga
     1921 tatcctcccg gcggccctgg acggcatcga tcaaggtgta gtggcgtccg caagggcagg
     1981 tcgcggtcga gaggcgcagg ctgtcgctca gctcatagcg gatcaacggg agggtgcggc
     2041 tggcgaggac ggtaatcagc agcttggcgc cgaaggtgcc gggagccacg gggcggttgt
     2101 gctcgtcgac cacctcgggg atcaccaggt cctcgaagag atgcatgccc cggtgcgcga
     2161 cacactcggc ggccaggccg ccgctttcgg tggcggcgta ctggttgaac ggctcctggc
     2221 cccaggcggc ggcaaccaac cgccgggttt catccgtcaa cacttcggag ctggtgaaca
     2281 ccagatgcgg ggtgatccgg agccgccccg cgagttgctc atcggccagg atgcgggcca
     2341 tcgaggcata gctgacgagc agatccggtt gccaagcgtt gagttgggcg acgatgtcgg
     2401 gcagcgggtc gctggccgcc aggcgcagtg ttggcatcca ccaactgcgc accgttgcgc
     2461 ccacggcggc agacatatgc cggggatcgg tcgaggcgac tgcggccagt ttcgtctgct
     2521 gggtcaagcg cacctgcgcg ccggcccact cctgggcgcg ggcaaaggag gcgagcacag
     2581 cagcccattc ggtccggtcg agcaggaaaa tgcccgggcg accactgctg ccggacgtag
     2641 cattgaccca gtaacggcca agaaagcgct ctgcttcgcg cgccccggcg acgtactgtc
     2701 gcaccgcctc cagatggacc gcccgatccg tgaccaggtc gtcaaaatgc tccattagca
     2761 tactcttggt caacaccggc agatcctgca gcggacgatc catgaggccg gcgtgaaact
     2821 gctggtagaa gggcgagcgg gcgtaggcgt attggcgcat gcgactcagg gcctgctcct
     2881 ggaacgtttg caattgctca cgtgtccacc ggtcgcgttt gcgtagttgc tccaggtcat
     2941 ggatcaagct ccacaggatt ggcatctcca tcgtcgtctc ctattccctg gtgggctccc
     3001 cgcgtccgcc gcatcccgtc atccttggct ggaggcacga ggcaaatcgc gatcttgctt
     3061 ccggatctgc tgggcggagc agacggagcc tgctccgcgg ggcggtagcg cccccgccgc
     3121 tgaaacgcac ctacgggctg tcctcgactc gcggcgtagc cgatcggccc aggtacaggg
     3181 acaatcccgc ggctcatcac gcgttcccac acacctccat actcgcgctc tcgcccttgt
     3241 cggcggccca cagcctatgg ccccatggag gaaccgggat cgtggacgag cagggtgccg
     3301 cgcaactccg gggttgtttg tgggtggtcg cgctgagtgc agacgaagtg ataggcgccg
     3361 ggaggcagcg gcggcagggt cagtgtggtc ga
//
LOCUS       DASUYC010000929         3309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1541444, whole genome shotgun sequence.
ACCESSION   DASUYC010000929 DASUYC010000000
VERSION     DASUYC010000929.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1541444"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="VF276_15200"
     CDS             <1..922
                     /locus_tag="VF276_15200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6608247.1"
                     /translation="VMATVQARLDGLDEPAVVARLAELDRQEGDLAAQMRQIREALAA
                     QEATIQGFLEKLEVQAPPAADRIAAAYPAFSTVSAADRPRLEESVDECQVTLGTTRGK
                     IRDLAQRLGLSMHEALDPVACAQELAACAHDIEVRKQAGRIVQAVRERMIQQVLPHTA
                     RNMRLLIPLLTLDRYRDCRITEDYKLEIWDEGAGRYVAKSIFSGGTRDQFSLALRLAF
                     ALATLPQELGTTPGFIFLDEPLSAFDGPRTQALITLLTQGAIHANFNQIFVISHNRMF
                     DRQAFTHHIRMEGGRVADHDLPRLQGAAAG"
     gene            complement(919..2106)
                     /locus_tag="VF276_15205"
     CDS             complement(919..2106)
                     /locus_tag="VF276_15205"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX6608248.1"
                     /translation="MVSPATVRIVATGDNHLNRFYDRMPPQKLEERRSYLRQGFQAAV
                     DHALSWPAHLFCITGDLFDQSDPRNVDRAFVADCLERLRTAGVRVYAVSGNHDTPRHS
                     GQQGGTTPYEVYSYLGALHYFDRIAEIETDVVEIAGLRLAIGGVCPDPAAPAGSDPLA
                     GIVWSNRPADADLGILLLHSPLEGHMPPDWETPQIARQTLETLDGADLILIGDSHQPY
                     TQQIGSRTVIAPGATECMTFGENGDLPGFVALELGRGPRITWRRIGLQGQPRAELHVR
                     VADLDADDPLADLIRRVRAICTPETLVKLHLEGIVTRDRYHALSLRPLHEAIQPHAFH
                     FTVDTAGLYLEDEQHQVAERGVRASQPEAIRVYAAELLASAGGSDERTAIEEALNRLL
                     ARY"
     gene            complement(2100..>3309)
                     /locus_tag="VF276_15210"
     CDS             complement(2100..>3309)
                     /locus_tag="VF276_15210"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX6608249.1"
                     /translation="RVAVYKVGGDSTDPLPPGVRSIDPFAGAGLLSADQPDSVLVLGG
                     TPGGLYNLIRRQAGGTLDWTIKWFDLLVIDEASQMSLPEAVLAGAFLRADGQAIVVGD
                     HRQMPPILAHTWKRERSRAALDYEPQRSLFESLKDINCPIVGLDESFRLHRVHAAFLA
                     QHIYHADGIAFHSRRQDLLQILPGRVDPYVAAALDPAYPVIVIEHDEAASQQLNPVEI
                     ALAEPLIRACTEDLQLNARDGLGVVVPHRAQKAALGRAFPALATAQAIDTVERFQGGE
                     REVILVSTTASEPGYLLTEAEFLFNRNRLNVAISRPRRKLIVLAARNVFRLLTDDPDL
                     FEHILLWKFLRYQHTDTLLWEGTHAGAAVRVFGRHCGHPIPPLAPAIPLVTSIPQSTY
                     TRRERRKEKW"
BASE COUNT          582 a         1037 c         1122 g          568 t
ORIGIN      
        1 ggtgatggcg acggtgcagg cgcggctgga tggcctcgac gagccggcgg tagtcgctcg
       61 gttggcggaa ctcgaccggc aggagggcga tttggccgcc cagatgcgcc agattcgaga
      121 ggcccttgcc gcccaggagg caaccatcca gggattcctg gagaagctgg aagtccaggc
      181 gccgcccgcc gcggaccgga tcgctgcggc ctatccggca ttctcgaccg ttagcgccgc
      241 cgatcggccc cgcttggaag aatccgtaga cgaatgccag gtgacactcg gcacaacgcg
      301 gggaaagatc cgcgacctgg cgcagcgcct gggtctctct atgcacgagg ctttagatcc
      361 tgtggcatgc gcacaggagt tggccgcatg cgcgcacgat atcgaggtgc gtaagcaggc
      421 ggggcgcatt gtccaggcgg tgcgtgagcg gatgatccag caggtgttgc cgcacaccgc
      481 acgcaatatg cggctgctga tcccgctgct gactctggac cgctatcggg attgccggat
      541 cactgaggac tacaaactgg agatctggga cgagggggcg ggtcgctacg tcgccaagag
      601 catcttctcc ggcggcacgc gcgaccagtt ctcgctcgcc ctgcggctcg ccttcgcgct
      661 ggcaactctg ccgcaggaac tgggcaccac gccaggcttt attttcctgg acgaaccgct
      721 cagcgccttc gacggtccgc gcacccaggc cctgattact ctgctcacgc aaggcgccat
      781 ccacgccaac tttaaccaga ttttcgtcat cagccacaac cggatgttcg accgccaggc
      841 ctttacccac catatccgca tggagggggg gcgggtagcc gatcatgatc tgccccgcct
      901 ccagggcgcg gcggcgggct aatagcgggc gagcaggcgg ttgagggctt cctcgatcgc
      961 cgtgcgctcg tcggaaccgc ctgcgctcgc cagcagttcg gcagcataga cgcggatggc
     1021 ttccggctgg gaagcgcgga ccccgcgttc cgcgacctgg tgttgctcat cttccagata
     1081 gaggccggcg gtgtccaccg taaagtggaa ggcatgcggt tggatcgcct cgtgcaacgg
     1141 gcgcaagctc agggcatggt agcggtcgcg ggtgacgatg ccttcgagat gcagcttgac
     1201 cagcgtttcc ggggtgcaga tggcacgcac ccggcgaatc agatctgcta gagggtcgtc
     1261 cgcatccaaa tccgcgaccc ggacatggag ttcggcccgc ggctgcccct gaagtccaat
     1321 acgccgccag gtgatccggg gaccgcgccc cagttcgagg gcaacaaagc ctggtaggtc
     1381 gccgttctcg ccgaaagtca tgcactccgt cgcgccggga gcgatcacgg tgcgggagcc
     1441 gatctgttgg gtgtacggct ggtggctatc gccgatgagg atgaggtcgg ccccgtccag
     1501 ggtttcgagc gtctgccggg cgatctgggg cgtctcccaa tcgggcggca tgtgcccttc
     1561 cagcgggcta tgcagcagca ggatgcccag atcggcatcg gcggggcggt tgctccagac
     1621 gatccccgcg agtggatccg atccggcggg cgccgcagga tcggggcaga ctcccccgat
     1681 agcgaggcgc aggccggcaa tctccaccac atcggtctcg atctccgcaa tgcggtcgaa
     1741 atagtgcaag gcgccgagat agctgtagac ctcatagggc gtggtcccgc cctgctgccc
     1801 gctgtggcgg ggggtatcat ggttgccgct cacggcatag acccgcaccc cggcggtgcg
     1861 caggcgctcc aggcagtcgg ccacgaaggc ccggtccaca ttgcgcgggt cggactgatc
     1921 aaacaggtca ccggtgatgc agaacaagtg cgccggccag ctcagggcgt ggtccacggc
     1981 ggcctggaag ccctggcgca ggtagctccg acgctcctct agtttctgcg ggggcatccg
     2041 gtcgtaaaag cggttcaggt ggttatcgcc ggtcgctacg atgcgcaccg ttgctggact
     2101 taccatttct cttttctcct ctcgcggcgg gtataggttg actggggaat cgatgtcacg
     2161 agtgggatcg cgggcgctag gggcgggatg gggtggccgc agtgccgtcc gaagacacgc
     2221 acggcggcgc cggcgtgggt tccctcccac aggagcgtat cggtgtgctg gtagcgcagg
     2281 aacttccaga gcagtatatg ttcgaacagg tccggatcgt cggtgagcag gcggaagacg
     2341 ttgcgggccg ccagcacgat gagcttgcgg cgcggccgcg agatcgcgac gttgagccgg
     2401 ttgcgattga ataaaaactc cgcctcggtc aacagatagc ccggctccga ggcggtggtc
     2461 gagaccagga tcacctcccg ctctccgcct tgaaagcgct ccacggtatc gatagcttgg
     2521 gccgttgcca gagcagggaa agcccgaccc agcgcagcct tttgcgcccg atgcggcacc
     2581 accacaccca ggccgtcccg cgcgttcaac tgcagatcct cggtacaagc ccggattagt
     2641 ggttcggcca gcgcgatctc caccgggttc agttgctgag acgccgcttc gtcatgctcg
     2701 atcacgatca cggggtaggc cgggtcgagc gcggcagcga catacgggtc cacacggccg
     2761 gggaggattt gcagcagatc ttggcgccgg gaatggaagg cgatgccgtc ggcatggtag
     2821 atatgctgag cgagaaaggc ggcgtgcacc cgatgcagcc ggaaactttc atccagccct
     2881 acgatggggc aattgatgtc ctttaggctc tcgaacaagg accgctgcgg ctcgtagtcc
     2941 agggcggcac gggaacgctc ccgtttccag gtatgggcca ggatcggagg catctggcga
     3001 tggtcgccga ccacgatggc ctgcccatcc gcccgtagga aggcgccggc caacacggct
     3061 tcgggcagac tcatctggct ggcttcgtcg atcacaagca gatcaaacca tttgatcgtc
     3121 cagtccaagg tgccgcccgc ctgccgccgg atcagattgt acaggccgcc gggtgtgccg
     3181 ccgagcacca gcacggagtc gggctgatcc gcgcttaaca gcccggctcc tgcgaaagga
     3241 tcgatggatc gcacaccggg cggcagcgga tcggtggagt ccccacccac tttgtagacg
     3301 gcgacgcgg
//
LOCUS       DASUYC010000930         3198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1101990, whole genome shotgun sequence.
ACCESSION   DASUYC010000930 DASUYC010000000
VERSION     DASUYC010000930.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101990"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1668
                     /locus_tag="VF276_15215"
     CDS             <1..1668
                     /locus_tag="VF276_15215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608250.1"
                     /translation="LVRLLQAIHPLALAPEALAGELDVLDRLDLTPLYEREPEPAYLF
                     KHVITHEATYYLMPEGQRRPLHRAVAEWYEEAHAADLAAFYPLLAYHWRHALGDAPSD
                     PDLIAKTIDYMLQAGAQAARNNANPEAVRFYQDALALLARLPATPERDLRELEILLAQ
                     VGPLVVIRGPAAPVVREVYDRAYALAEAGAATPSMLPVLYGLIRFHVGRAEYQEARSL
                     ADHLVRLAESMGDAEQLGGSHYVGVAYTLFNLGELAAARAALEQAVRLYDPARHGHLA
                     FQLGRDPGEGALDLLAILLWVLGYPDQALDRSAQALALARQLNHPTTLGSALGYAAQL
                     RVLRGEAQAALAETETLLALSAEHAAPVLAVGGALYKARALAALGRTAEGMALFREGS
                     AIYESTGAAGTAQILCPLAEICLRAGQIAEGLAAADAGLRVAEDRGERWLEAELYRLR
                     GELLLAQDTPASKEALAEAERCFTEALAVARRQEAKGWELRAALSLGRLRQRQGQPAA
                     AQALVAEVYDRFTEGHDTGDLRAARDLLDEPAGTPRGDRSALGSCTR"
     gene            1656..2738
                     /locus_tag="VF276_15220"
     CDS             1656..2738
                     /locus_tag="VF276_15220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608251.1"
                     /translation="MHTVAQYLPPLRPALPPALIGAEAWAAIMAVAAQLPGALAHSVF
                     LFECRLGEPHPAADFLVCASARHGGREPLAQLAVPNSPAAALAAHPIWQRVVTFARAW
                     ADPGTPLHAALTDVWLEFDLPVAAPPLQLPSVFFGGPLLPDAGAPADRAASQLDRMGR
                     AIRLLTGHDLPAPVAGQLARCLTALPPGAQSPVAAVLPARNTGAIRLVLDGIPAAEVL
                     PYLERIGWPGPRDRVAQALPGLAARCDYLWLDLDVDAMPAPKVGVECVFHSGPAQAGW
                     PAFFAYLAELGLCRADKGAAVLAYPGTLAADAAGAAWPAPLREVAALLGPLGYRRLVR
                     EIHHVKLVFEADRPLEAKVYLAAYYE"
     gene            complement(2746..>3198)
                     /locus_tag="VF276_15225"
     CDS             complement(2746..>3198)
                     /locus_tag="VF276_15225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608252.1"
                     /translation="RTAFNGLGHAIWQVRQNPAEAESWRVVVLHAGLLDHGIRGLATY
                     RVLPDRIELEYMGAAQAGQGAGTVLLRYLAAEAAARHVPLTLSAYAGAAGFYRRHGMS
                     EAPARAGRPLARFTFAPAAAAHYAQTGRPLPPLVHHPESMPAAYRRVD"
BASE COUNT          395 a         1204 c         1160 g          439 t
ORIGIN      
        1 ctggtgcgcc tgctacaggc catccacccg ctggccctcg ccccggaggc cctggccggc
       61 gaactcgacg tgctcgaccg gctggatctg acgccgctgt atgagcggga gcccgagccg
      121 gcctacctgt tcaagcacgt catcacccac gaagcgacct actacctgat gccggagggg
      181 cagcggcggc ccctgcaccg cgccgtggcc gagtggtacg aggaagcgca cgccgcggac
      241 ctggccgcct tctatcccct gctggcctac cactggcggc acgcgctggg cgatgcgccg
      301 tccgacccgg acctgatcgc caaaaccatc gactacatgc tgcaggccgg ggcgcaggcg
      361 gcgcgcaaca acgccaaccc ggaagcggtg cgcttctacc aggacgcgct ggcgctgctg
      421 gcccggttgc ccgcgacgcc ggagcgcgac ttacgcgaac tggagattct gctcgcccag
      481 gtggggccgc tggtcgtcat ccgcggcccg gcggcgcccg tcgtacgcga ggtctatgac
      541 cgggcctatg ccctggccga ggccggcgcg gccaccccca gcatgttgcc cgtgctctat
      601 ggcctgatcc gcttccacgt cgggcgggcc gagtaccagg aggcgcgcag cctggccgac
      661 cacctggtcc gcctcgccga gagcatgggc gacgcggagc aactcggcgg cagccactac
      721 gtgggtgtgg cttataccct gtttaacctg ggcgaactgg cggcggcgcg ggcggcgctg
      781 gagcaagccg tgcggctcta cgatccggcc cggcacggcc acctggcgtt ccagctcggg
      841 cgcgatcccg gcgagggcgc gctcgacctg ctggcgatcc tgctgtgggt cctgggctac
      901 ccggatcagg cgctggatcg cagcgcccag gcgctggcgc tcgcccgcca gttgaatcac
      961 ccgaccacgc tcggctcggc cctcggctac gccgcgcaac tgcgggtgct gcgcggcgag
     1021 gcccaggccg cgctggccga gaccgagacg ctgctggcgc tgtccgccga gcacgccgcg
     1081 ccggtgctgg ccgtgggcgg cgcgctctac aaggcgcgcg cgctggccgc gctaggccgg
     1141 accgccgagg ggatggcgct cttccgcgag gggtccgcca tctatgaatc cacgggcgcc
     1201 gccggcaccg cccagatcct gtgcccgctg gccgagatct gcctgcgcgc gggccagatc
     1261 gccgagggcc tggccgccgc ggacgcgggg ctgcgcgtgg ccgaggaccg cggcgagcgc
     1321 tggttggaag ccgaactcta ccgcctgcgc ggcgagttgc tgctggccca ggacacgcct
     1381 gcttctaaag aggcactggc cgaagccgaa cgctgtttca ccgaggcgct ggccgtggcc
     1441 cgccggcagg aggcgaaggg ctgggagttg cgcgccgccc tgagcctggg ccgcctgcgg
     1501 cagcgccagg ggcaacccgc cgccgcccag gccctggtgg ccgaggtcta cgaccggttc
     1561 accgaaggcc acgacaccgg cgatctgcgc gccgcgcgcg acctgctgga cgagccggcg
     1621 ggcacacccc gcggcgaccg gagcgcgctc ggctcgtgca cacggtagcg cagtatttgc
     1681 cgccgctgcg gcccgcgttg ccgcccgcgt tgatcggcgc ggaggcgtgg gccgccatca
     1741 tggcggtggc ggcgcagttg cccggcgcgc tggcgcatag cgtcttcctg ttcgagtgcc
     1801 ggctcggcga gccccacccg gcagccgatt tcctggtctg tgccagcgcg cgccacggcg
     1861 ggcgcgagcc cctggcccaa ctggccgtgc cgaatagtcc cgccgccgcc ctggccgccc
     1921 accccatctg gcagcgcgtc gtcacgtttg cccgtgcgtg ggccgatccc ggcacgccgc
     1981 tgcatgccgc cctgaccgat gtctggctcg aattcgacct gcccgtcgcg gccccgcccc
     2041 tccagctgcc gagcgtcttc tttggcggcc ccttgctgcc ggacgccggc gcacccgccg
     2101 atcgcgcagc gtcgcaactc gaccgcatgg gccgtgcgat ccggctgctg accgggcacg
     2161 atctaccggc gccggtggcg gggcaactgg cacgctgcct caccgcgctg ccgccgggcg
     2221 cccaatcgcc ggtcgccgcc gtgctgccgg cccgcaacac cggcgccatc cggctggtgc
     2281 tggacggcat tcccgccgcg gaggtcctgc cctacctgga gcgcatcggc tggcccggcc
     2341 cgcgcgaccg ggtggctcag gcgctgcccg gcctcgccgc gcgctgcgat tacctctggc
     2401 tcgatctcga tgtggacgcc atgccggcgc cgaaggtggg cgtggaatgt gtgttccaca
     2461 gcggcccggc ccaggccggc tggccggctt tcttcgccta tctggccgaa ctcgggctgt
     2521 gccgggcgga caaaggcgcc gccgtgctgg cctatccggg gactctcgcg gcggacgcgg
     2581 cgggcgccgc ctggcccgcg cccctgcgcg aggtcgccgc cctgctcggc cccctcggct
     2641 accgccgcct ggtccgcgag atccaccacg tgaagctcgt gttcgaggcg gatcggcctc
     2701 tggaagccaa ggtctatctg gcggcgtatt acgagtgacc ggcagctaat cgacgcggcg
     2761 gtaagcggcg ggcatggatt cggggtggtg gaccagaggc ggcaggggcc ggcccgtctg
     2821 cgcgtagtgc gcggcggcgg cgggggcgaa ggtgaagcgg gccagcggcc ggcccgcccg
     2881 cgccggggcc tcgctcatgc cgtggcggcg atagaagccc gccgcgccgg cgtaggccga
     2941 gagcgtcagc gggacatggc gggcggcggc ctcggcggcc aggtagcgca gtagcaccgt
     3001 gcccgcgccc tgcccagcct gggcggcgcc catgtattcc agttcgatcc ggtcgggcag
     3061 cacgcggtaa gtcgccagcc cgcggatgcc gtggtccagc agtccggcat gcagcacgac
     3121 cacccgccac gactcggcct cggcgggatt ctgccgcacc tgccagatag cgtgccccaa
     3181 cccgttgaac gccgtgcg
//
LOCUS       DASUYC010000931         2270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1915759, whole genome shotgun sequence.
ACCESSION   DASUYC010000931 DASUYC010000000
VERSION     DASUYC010000931.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1915759"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2223)
                     /locus_tag="VF276_15230"
     CDS             complement(<1..2223)
                     /locus_tag="VF276_15230"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NB-ARC domain-containing protein"
                     /protein_id="HEX6608253.1"
                     /translation="MDRQISFGAWVKQQRKAADLTQDAFAERVGCSGEMIRKIEAGTA
                     RPSRQMAELVVAHLGIPESDQAAYVQWAREGIPPVLPPAPPPTTPASRTPAPHAPGSV
                     VLTAPAPPVALSPAFVTAPHTNLPFYPTPLVGREAEIAAVCRLLRQSDVRLVTLTGPP
                     GVGKTRLAVQAAARLLDDYACGVWFVSLAPIRNPGLVASAVAATLRITETAGRSLAEM
                     LADYLRDKSLLLVLDNFEQVLDAGALVAALLAATTRLKVLVTSRAALRLYGEHEFPVP
                     PLAVPEANGPWTVAHLGTYDAVELFRQRADAVDPGFVLTDDTGPPVGEICRRLDGLPL
                     AIELAAARCKLFPPAALLARLDHHLPVLTGGPRDQPARHQTLRAALAWSYDLLSPAEQ
                     RMLRRLAVFSGGCTAEAAAAVCTEAGENPDEVPACLEELVTQSLVVCRPRGGTLRYGL
                     LETIREYAWDQLAAEGEATEARDHQLRYYAQLVVDIEPELFGAKHVEMHARLGQEYNN
                     LRAAVGWATAEGGNEEGLKLVGALWHYWNWAGFESEARVWVNQLLAAAPPARPSRVRA
                     KALHAAGRLAWRCADYAEARARYDEALALFQALDDKSGTGLVLNGLGLLVMDDEQDYR
                     CAHKFFEEGLAARRAAGDRWGIAHSLSCLGGCLALEGDYPAARAAQEEALSIWRELGD
                     VLSIAWQSLLLGVPNLYGDCCEQAQQRAEEGLAIFRQVGNKRGIANGLYQLSYLARWQ
                     G"
BASE COUNT          351 a          806 c          779 g          334 t
ORIGIN      
        1 cccctgccat cgggccaggt agcttagctg gtaaagccca ttcgcaatgc cccgtttgtt
       61 gcccacctgc cggaagatgg ccaacccttc ctccgcccgc tgctgcgctt gttcgcaaca
      121 gtcgccatac aagttcggca cgcccagcag cagggattgc cacgcgatac tcaacacatc
      181 gcccagctcg cgccagatgc tcagcgcctc ttcctgcgct gcccgcgccg cgggataatc
      241 gccttcgagt gcaagacatc cgccgagaca ggaaagagaa tgggcgatgc cccaccgatc
      301 cccggctgcg cggcgcgcag ccagcccttc ctcgaaaaac ttgtgcgcgc accggtaatc
      361 ttgttcatcg tccattacca acaggccgag cccgttcaac accaacccgg tgcccgactt
      421 gtcgtccagc gcctggaaca gggcgagggc ctcgtcgtaa cgggcccgcg cttcggcata
      481 gtcggcacag cgccaggcca ggcgcccagc cgcgtgcagc gccttcgcac gcacgcggct
      541 gggccgggcg ggaggtgcgg cggccagcaa ttggttgacc cagacccgcg cttcgctctc
      601 aaacccggcc cagttccaat aatgccagag cgcgccgacc agtttcaacc cctcctcgtt
      661 gccgccctcg gcggtggccc agccgacggc cgcgcggagg ttgttgtact cctgccccag
      721 gcgggcgtgc atctcgacat gcttggcacc gaacaattcg ggttcgatgt ccaccaccag
      781 ttgcgcgtaa tagcgcaact ggtggtcgcg cgcctccgtg gcttcgccct ccgccgccag
      841 ttggtcccag gcgtattcgc gaatggtctc cagcaaaccg tagcggaggg tcccgccccg
      901 cgggcggcac acgaccaacg attgggtgac cagttcctcc aggcacgccg gcacctcgtc
      961 cgggttctcg cccgcttcgg tgcacaccgc cgcggcggcc tccgcagtac agccgccgga
     1021 gaagaccgcc aaccggcgca acatccgctg ttcggcgggc gacagcaagt cgtagctcca
     1081 agcgagggca gcgcgcaagg tctggtgccg ggcgggctgg tcgcgtggtc cgccggtcag
     1141 caccggcaag tggtgatcga gccgggccag cagcgcggcg ggcgggaaga gcttgcagcg
     1201 ggccgcggcc agctcgatcg ccagtggcag cccgtccagc cggcggcaga tctcgcccac
     1261 gggcgggccg gtgtcgtcgg tgagcacgaa accggggtcg actgcgtcgg cccgctgccg
     1321 gaacaactcc accgcgtcgt acgtgcccag gtgggccacg gtccacggcc cgttcgcctc
     1381 gggcacggcc agcggtggca ccgggaactc gtgttcgccg tagaggcgca gtgccgcccg
     1441 gctggtgacc agcaccttga gccgggtggt ggccgccagc agtgccgcga ccagggcccc
     1501 ggcgtccagc acttgctcga agttgtcgag caccagcagc agggacttgt cgcgcaggta
     1561 gtcggccagc atttcggcca gggaccggcc cgccgtctcc gtgatccgca gcgtcgcggc
     1621 aacggcggag gccacgaggc cgggattgcg gatgggcgcg agggacacga accagacgcc
     1681 gcaggcgtag tcgtcgagca ggcgggcggc ggcctggacc gccaggcggg tcttgcccac
     1741 acccggcggg ccggtgaggg tcacgaggcg cacatcggac tgccggagca gccggcacac
     1801 cgccgcgatc tcggcctcgc ggcccacgag cggcgtgggg tagaagggca aattggtgtg
     1861 cggcgcggtt acgaacgccg gtgagagcgc caccggcggc gcgggggcgg tgagtactac
     1921 gctgcccggc gcgtgcggtg ccggggtgcg cgaagccggg gtagtgggcg gcggggccgg
     1981 gggcagcaca ggcgggatgc cctcccgcgc ccattggacg tatgccgcct gatcgctttc
     2041 cgggatgccc aggtgggcca cgaccagttc ggccatctgc cgcgacgggc gggccgtgcc
     2101 cgcctcgatc ttgcggatca tctcgccgga gcagccgacg cgttcggcga atgcatcctg
     2161 ggtcaggtcg gcggccttgc gctgctgttt gacccaggcg ccaaaggaaa tctgcctatc
     2221 catcggcgtc cctcctctcg cggtctccta cccgagcacg agacgccttt
//
LOCUS       DASUYC010000932         2024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_2003454, whole genome shotgun sequence.
ACCESSION   DASUYC010000932 DASUYC010000000
VERSION     DASUYC010000932.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2003454"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="VF276_15235"
     CDS             <1..852
                     /locus_tag="VF276_15235"
                     /inference="COORDINATES: protein motif:HMM:NF013583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HEX6608254.1"
                     /translation="APGRGGYDQSSNRGRGGRRRGGGGSYQGGGGQAGYDRMGGGRAG
                     GGMTSMAALSPGPSRMTSRAAPVSDYYGERSERQAATARYTVYEDDRGDDDDPAPVAL
                     APVSVAAPPAPRAPGVPGSSTKPLRIYPFGVSRNRLEQAIGGLNVDVTLSKDIADADI
                     VLTLKNYYRKKPPALRQAEAEGLPVYVLKSNTASQMQGILSNIFELAPGSAAAAGGDE
                     DPVTNAIEETEAAITEALETGHPVELPPANAYIRRLQHQMAERYNLGSQSRGREPNRR
                     VRIFRHE"
     gene            845..1189
                     /locus_tag="VF276_15240"
     CDS             845..1189
                     /locus_tag="VF276_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEX6608255.1"
                     /translation="MNDSPKKLVIVVIQGQDIGAVRRALPAAGFGMTEVDSLGGFLRE
                     NNVTLLIGTEESRLADLWRLLAENCLTRTEYISPFQPAYGPGDVFLPRPVEVQVGGAT
                     VWVVDVERFANL"
     gene            1217..1561
                     /locus_tag="VF276_15245"
     CDS             1217..1561
                     /locus_tag="VF276_15245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608256.1"
                     /translation="MSCPFAGLATRYTMRDERVSVRICGAVPYERDSDPEDLPLPSVC
                     FSHAAWESCAHYLRLTQRREVARRLGLPLRDGSVPDEEDLAAEVITPMFEFPELPAEP
                     DPREDPKPSGAD"
     gene            1565..2005
                     /locus_tag="VF276_15250"
     CDS             1565..2005
                     /locus_tag="VF276_15250"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX6608257.1"
                     /translation="MPLITIPDLSGFVPAFQLFVACTGAYLVALWLSLIIWTFRDIQG
                     RTRDVISMALSTLLVAIFTVPGLLLYLILRPKETLSESYERSLEEEYLLQDIEDAEIC
                     PACKHRVERDFMYCPHCRTRLRRECEGCGSPVSLRWAACPHCGR"
BASE COUNT          323 a          681 c          700 g          320 t
ORIGIN      
        1 gcccctgggc gcggcggcta cgaccagtcg tctaaccgcg ggcgcggcgg gcggcggcgc
       61 ggcggcggcg ggtcctacca gggtggcggc gggcaggcgg gctacgaccg catgggcggc
      121 gggcgggccg ggggcggcat gaccagcatg gccgcgctga gtcccggccc ctcgcgcatg
      181 acctcgcgcg ccgcgcccgt gagcgattat tacggcgaac gcagcgagcg ccaggcggcg
      241 accgcccgct ataccgtcta tgaggacgat cgcggggatg acgacgatcc cgccccggtg
      301 gcgctcgccc cggtcagcgt ggccgcgccg cctgcccccc gcgcgccggg cgtgcccggc
      361 agtagcacca agccgctgcg tatctaccct tttggggtca gccgcaaccg gttggaacag
      421 gccatcggcg ggctgaacgt cgatgtgact ctgagcaagg acatcgccga cgccgacatc
      481 gtgctgacgc tgaagaacta ctatcgcaag aagccgcccg cgctgcgcca ggccgaggcg
      541 gagggcttgc cggtgtacgt gctcaagagc aacacggcga gccagatgca gggtatacta
      601 tccaatatct ttgagctggc ccccggcagc gccgccgcgg cgggcggcga tgaggacccg
      661 gtgacgaacg cgatcgagga gaccgaggcg gccatcacgg aagccctgga gaccggccac
      721 ccggtggaac tcccgcccgc gaacgcctac attcgccggc tgcaacatca gatggccgaa
      781 cgctacaacc tggggtcgca gagccgcggg cgcgagccta atcgccgggt acggatcttc
      841 cgccatgaat gatagcccca agaaactggt gatcgtggtg attcagggcc aggacatcgg
      901 cgcggtgcgc cgggcgcttc cggcggcggg cttcggcatg accgaggtgg acagcctggg
      961 cgggttcctg cgcgagaaca acgtcacctt gctgatcggg accgaggagt cgcgcctggc
     1021 cgacctgtgg cggctgctgg ccgagaactg cctgacgcgc accgaatata tcagcccctt
     1081 ccaaccggcg tacgggccgg gcgatgtctt cctcccccgc cccgtggagg tgcaggtggg
     1141 aggcgccact gtctgggtgg tggacgtgga gcgcttcgcc aatctgtgac ggctccgcgt
     1201 ggaggagaag aggaccatga gctgcccgtt cgccggactg gcaacccgct acaccatgcg
     1261 cgatgaacgt gtgagcgtgc gcatttgcgg cgccgtgccc tatgagcgcg actccgatcc
     1321 ggaggacctg ccgctgccca gcgtctgttt cagccacgcc gcctgggaaa gctgcgccca
     1381 ctacctgcgg ctgacccagc gccgggaggt ggcgcgacgc ctggggctgc cgttgcgcga
     1441 cgggtccgtg ccggacgagg aggatctggc ggcggaggtg atcaccccga tgtttgagtt
     1501 cccggaactc cctgcggagc ctgatccgcg ggaagacccg aagccgtccg gagccgacta
     1561 atctatgccg ctgattacca tacccgattt aagcgggttc gtgcccgcct tccagttgtt
     1621 tgtcgcgtgt accggggcct atctcgtcgc cctctggctc agcctgatca tctggacctt
     1681 ccgcgatatt caaggccgca cccgcgacgt gatctccatg gcgctgtcga cgctgctggt
     1741 ggcgatcttc accgtgcccg gcctgctgct ctacctgatc ctgcggccca aggagacgct
     1801 gtccgagtcg tatgagcggt cgctggaaga agagtatttg ctgcaagaca tcgaggacgc
     1861 cgagatctgc cccgcctgca agcaccgcgt cgagcgcgat ttcatgtact gcccccattg
     1921 ccgcacccgg ctgcgccgcg aatgcgaggg ctgcggtagc ccggtgagct tgcgctgggc
     1981 cgcctgcccg cactgtggcc gctaaaaccc gcatcctcgg tttc
//
LOCUS       DASUYC010000933         2175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_913266,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000933 DASUYC010000000
VERSION     DASUYC010000933.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913266"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VF276_15255"
     CDS             complement(<1..369)
                     /locus_tag="VF276_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEX6608258.1"
                     /translation="MPAVPSPAPAPPPADDWLFGWDPTPGIVSVWADRAGRALVWQRG
                     AGGVTCAAARFRPWLWAATLDDLAHLGPALVPVDAPGAADAPVCYRPLADATTGTYRY
                     LLSARDGPTLERTILTGATRR"
     gene            complement(649..1137)
                     /locus_tag="VF276_15260"
     CDS             complement(649..1137)
                     /locus_tag="VF276_15260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608259.1"
                     /translation="MDDKSTQQGTGTTGATGTGGTSGADPREELRQQLEAMGKAAGDL
                     LEQLVKVPATLAQIPMQALPEDAATHARNAASEGFAAVKTLLDSMAKGVDEVMKAQRE
                     RMANMGSTHTGVGGPTSTSAGSDVSGQDTDYTQRLDSGMSDTAGRDINNGGNTVRLDE
                     EP"
     gene            complement(1304..>2175)
                     /locus_tag="VF276_15265"
     CDS             complement(1304..>2175)
                     /locus_tag="VF276_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HEX6608260.1"
                     /translation="TLWMLDTGRLDPGALAAGLALSAVIGAVAYAAGSLTAGGVLGAL
                     LIGTATFAFGGLAWGLLLIVFFATSSALGRVGARAARKAEAARAFAKGGRRDLGQALA
                     NGGVAGLLAVGAAAAPPEWQAPLFAAFAGSLAAVTADTWGTELGTLSRTAPRRVTTGR
                     VVPAGTSGGVSGLGLLAGAAGAALIGLAAWGFIGLWPLRGAPPGETPLLAAALLGGLG
                     GTLFDSLLGATVQQVYWCPQCGKETERRVHSCGTPTRPLRGRPWADNEIVNLGCALAG
                     AGLGLLVALLGRG"
BASE COUNT          303 a          793 c          801 g          278 t
ORIGIN      
        1 gcggcgggtg gccccggtga ggatcgtgcg ctccagggtg gggccatcgc gggccgacag
       61 cagatagcga taggtgcccg tcgtcgcatc ggccagcggc cggtagcaga ccggcgcatc
      121 cgccgcgccg ggcgcgtcaa cgggtaccag cgccggcccc aggtgggcca ggtcgtcgag
      181 ggtcgccgcc cacagccagg ggcggaagcg ggcggcggcg caggtcactc cgcccgcccc
      241 ccgctgccag accagggcgc ggcccgcccg gtcggcccag accgagacga taccgggggt
      301 gggatcccag ccgaacagcc agtcgtcggc gggcgggggt gcgggggctg gggacggcac
      361 ggcgggcatc gatctcctgg gcatgcggac ggcagatagg cttcagggag agcatagcat
      421 atcgcgctgg acgcgacaag catggcccgg aggctcaagc ctccggctac gtttacgaag
      481 gccgcctgca cggcctgtct cgcgctagtc ccagcccgcg caggcgggct tcgtcgcgct
      541 agcccgccca tttatgggcc gggcgctgcc gacgcacacg gcacggcgca aaaaaagacc
      601 tctcccgcgc ggggagaggt cctggcgtat gcgggatgcg cgtggcgctt acggttcctc
      661 gtccaggcgg acggtgttgc cgccattgtt aatatcgcgc ccggccgtgt cagacatgcc
      721 gctgtcgagg cgctgggtgt agtcagtgtc ctggccggag acatcactac ccgcggacgt
      781 actggtgggg ccgccgacgc cggtgtgggt gctgcccatg ttcgccatgc gctcgcgctg
      841 ggccttcatc acctcgtcca cgcccttcgc catgctgtcg agcagggtct tcacggcggc
      901 gaagccttcg ctggccgcgt tgcgcgcgtg ggtggcggcg tcctccggca acgcctgcat
      961 ggggatctgc gcgagggtgg cgggcacctt caccagttgc tccagcaagt cgccggccgc
     1021 cttgcccatc gcctccaact gctggcgcaa ctcttcgcgc ggatcggccc cgctcgtgcc
     1081 acccgtgccg gtggcgccgg tagtgccggt gccctgctgc gtgctcttgt cgtccatgaa
     1141 aagctccttt cgctgtccag agaccatctg gacatgatct gaccgtcata agaggcgcca
     1201 cggcagccgc gacgccggtg cccaggcgcg atactgccct gcctggctcc acctgttatt
     1261 acgtgaccac cccaccaaac gttacacgga ggcgcgcttt cggtcagccg cgccccagca
     1321 gcgcgacgag caggcccagg cccgcgcccg ccagggcaca gccgagattg acgatctcgt
     1381 tgtcggccca ggggcggccc cgcagcgggc gggtgggcgt gccgcagcta tggacgcgcc
     1441 gctcggtctc cttgccgcac tgggggcacc agtagacctg ctgcacggtg gcgcccagca
     1501 gggagtcgaa cagggtgccg ccgagcccgc ccagcaacgc ggcggccagc agcggcgtct
     1561 cgccgggcgg ggcgccacgc agcggccaga gcccgatgaa gccccacgcg gccagcccga
     1621 tgagcgcggc cccggcggct ccggccagca agccgagccc gctcacgccg ccggacgtgc
     1681 cggcgggcac gacgcggccc gtggtcacgc ggcggggcgc ggtccggctg agggtgccga
     1741 gttcggtgcc ccaggtatcg gcggtgacgg cggcgaggct gccggcgaag gcggcgaaca
     1801 gcggggcctg ccattcgggc ggggccgcgg cggcgcccac ggccagcagc ccggccaccc
     1861 cgccgttcgc cagcgcctgg ccgagatcgc ggcgcccgcc cttggcaaag gcgcgggcgg
     1921 cttcggcttt gcgcgcggcg cgcgccccca cccggcccag cgcgctcgac gtagcgaaga
     1981 agacgatcag cagcaggccc caggccaggc cgccgaaggc gaaagtggcg gtgccgatga
     2041 gcagcgcccc cagcaccccg cccgcggtca ggctgcccgc ggcataggcg accgccccga
     2101 tgacggcgct gagggccagc ccggcggcca gcgcgcccgg atcgagccgc ccggtgtcga
     2161 gcatccagag cgtca
//
LOCUS       DASUYC010000934         3909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_408932,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000934 DASUYC010000000
VERSION     DASUYC010000934.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408932"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="VF276_15270"
     CDS             <1..1041
                     /locus_tag="VF276_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HEX6608261.1"
                     /translation="IEAGHPLPNAHGVAGTEQILARLGGLGPRDLVLALISGGGSALL
                     ADPAPGLTLDVLGDVTNRLLRAGATIGELNTVRKHLSALKGGQLAGRAAPATVVALIL
                     SDVVGSPLDVIASGPTVPDPSTYADALAVLDRHALRGDIPVGARTHLEAGAAGTISET
                     PKPGDPLFERVITHIIGDNRRAALAAVEAGRGLGLHAALLSTYIEGEAREVGRVLAGI
                     AKELHASAQPWPRPACLVAGGETTVTVRGDGQGGRNQEVALGAALALDGWGTGVAVAT
                     LATDGGDGSGDAAGAFADGTTVARARAAGLDPQAALAANDSFHFWQALGDAVVTGPTG
                     TNVNDLAFILAV"
     gene            1183..2427
                     /locus_tag="VF276_15275"
     CDS             1183..2427
                     /locus_tag="VF276_15275"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX6608262.1"
                     /translation="MMNTKNTTILISGASIAGPTLAYWLKRYGFTPTVVERAPHLRAG
                     GYPVDVRFEAVQVARLMGIWPRLQQEQTTLGEISFVNERHQRIGRVNLQAGRKALEAF
                     DVDTVEIMRGDLAKILYDLTKNEVEYRFGDSIRELRQDEEGVEVTFARGGSRRFDLVV
                     GADGLHSIVRTLVFGDAAHFERYLGYQVGVFTTDNDLGLGEACMQMYGVPGKQVAVRS
                     TRGNQTLAAYFLFKQPTQLRYDYHDVAQHKQLLAAVFAGEGWEVPRLLEKMQTASDLY
                     FDAVSQIRMKRWSRGRAVLLGDAGYCPALLTGQGSTLAMMGAYILAGELKVALGDHNL
                     AFVRYEQAFRPIVLQEQNRAGANARVLVPATPVGLWVQTHLVPLLVPPLLWAMERGRR
                     LLPKRSSRPSRIKDYGNVLRPS"
     gene            2653..>3909
                     /locus_tag="VF276_15280"
     CDS             2653..>3909
                     /locus_tag="VF276_15280"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein"
                     /protein_id="HEX6608263.1"
                     /translation="MKQGGFTFVLHSHLPFYRQTGRWPHGEEMLHEAMAETYIPLLDA
                     LLDLAAEGVPYRLTLGITPVLAEQLRDPLIGEHFDRYIAHELALADSDRERAENRGDS
                     AGAALARYYEGYYTRAGRVFNERLGRDILGAARRLQDAGYIEILTSAATHAYLPLLSR
                     DSSIHAQIAVGKQTYERLYGRAPRAIWLPECAYRPAYVTHSESRGGYVKPGIESFLAA
                     AGIGLFFCETHAVTGGDPAGKAGGGAIGYAPIPDRLLVPIPNYIPPTNRTTYLPYLVG
                     RSDVAVMARNERTGQQVWAAQIGYPGDPWYREFHRKDLNSGMQYWRITGHEVPLGLKA
                     PYDPAHAQQRVREHAHHFTGLVEDLLRQYHEAGGAPGLIVSAYDTELFGHWWHEGVDW
                     LKATLRNLAASPAVELTTASAWLAAHP"
BASE COUNT          665 a         1333 c         1320 g          591 t
ORIGIN      
        1 atcgaagcag gccacccgtt gccgaatgcg cacggcgtgg cgggcacgga gcagatcctg
       61 gcacgactgg gcggcctggg gccgcgcgac ctggtgctgg cgctcatctc cggcggcggc
      121 tcggcgctgc tggccgaccc cgcgcccggc ctcaccctcg acgtgctcgg cgatgtcacc
      181 aaccgcctgc tgcgggcggg cgcgaccatc ggcgaactca acaccgtgcg caagcacctg
      241 tcggcgctca agggcggcca actggccggg cgcgcggccc ccgccacggt cgtcgccctg
      301 atcctcagcg atgtcgtcgg cagcccgctc gacgtgatcg cttccggccc caccgtgccc
      361 gaccccagca cctacgccga tgccctggcc gtgctcgacc gccacgcgct ccgcggcgac
      421 atcccggtag gggcgcgcac gcacctggag gcgggcgccg ccggcacgat ctccgaaaca
      481 cccaagcccg gcgaccctct gttcgagcgg gtgatcaccc atattatcgg cgacaaccgc
      541 cgggcggcgc tggccgccgt cgaggccggg cggggcctgg ggctgcacgc cgcgctgctc
      601 tccacctata tcgaaggcga ggcgcgcgag gtggggcgcg tgctggccgg gatcgccaag
      661 gaactccacg cgagcgctca gccctggccc cgcccggcct gcctggtggc gggcggcgag
      721 accaccgtga ccgtgcgcgg cgacggccag ggcgggcgca accaggaggt ggccctgggc
      781 gccgcgctgg ccctcgacgg ctggggcacc ggggtcgccg tcgccacgct ggcgaccgac
      841 ggcggcgacg gcagcggcga tgccgcgggc gccttcgccg acggcacgac cgtggcccgc
      901 gcccgcgccg ccgggctcga cccgcaggcc gccctggccg ccaacgacag cttccacttc
      961 tggcaggccc tcggcgacgc cgtggtcacg gggcccacgg gcaccaacgt caacgacctg
     1021 gcctttatcc tggccgtatg atgagaattg ggcatgagca gggtgatgta atggcttgag
     1081 cgcccattgg tcaatcccgt tctgcaattg ggagccgggt acagaacgaa caaggcgtgt
     1141 cgccgcctgc aaatgtgttc ctctttccag agggagagca ccatgatgaa caccaaaaac
     1201 accactattc tcatttctgg agcgagtatt gccgggccaa ccctggccta ctggctcaag
     1261 cgctatgggt ttaccccgac cgtggtcgag cgtgcgcccc acctgcgagc aggcgggtac
     1321 ccggttgacg tgcgctttga ggccgtgcag gtagccaggc tgatggggat ctggccgcgc
     1381 ctgcagcaag agcagacgac cctgggcgag atctcgtttg tcaacgaacg ccatcagcgc
     1441 atcggcagag tcaacctgca agccgggcgc aaggccttag aggccttcga cgtggatacg
     1501 gtggagatca tgcggggcga tctggccaag atcctctacg acctgacgaa gaacgaagtg
     1561 gaataccgct tcggggactc tatccgggag cttcggcagg acgaggaagg agtcgaggtg
     1621 acgtttgcgc ggggcggctc ccgccgcttc gacctggtgg tgggggcgga cgggctgcat
     1681 tcgatcgtgc gcacgctggt gtttggcgac gcagcgcact ttgagcgcta tttaggctac
     1741 caggtcgggg tcttcacgac cgacaacgat ctgggactgg gcgaggcctg catgcagatg
     1801 tatggtgtcc ccggaaaaca ggtcgcggta cgcagtacac gaggcaacca gaccctggcc
     1861 gcgtacttcc tgttcaagca acccacgcag ttacgctatg attaccatga tgtggcgcag
     1921 cacaagcaac tgctggcggc ggtgtttgcc ggcgaaggct gggaagtccc gcgactcctg
     1981 gaaaagatgc aaacggcctc cgacttgtac ttcgatgcgg tgagccagat tcgcatgaag
     2041 agatggtccc gcggacgagc ggtgctgctg ggcgacgccg gctattgccc ggccctgctg
     2101 acggggcaag gctcgaccct ggctatgatg ggcgcctata tcctggcggg agaactgaag
     2161 gtggcgctgg gcgatcacaa cctcgcgttt gtccggtacg aacaggcctt cagacccata
     2221 gtcctacagg agcagaacag ggctggagca aatgccaggg tcctggtgcc cgcaacgcct
     2281 gtcggcctgt gggtgcaaac gcacctggtc ccgctgctgg taccgccgct gctgtgggca
     2341 atggagcgcg ggaggcgatt gctccccaag cgctcctcaa ggcccagcag gataaaggac
     2401 tatgggaatg tgctccggcc atcttaacaa gcgccgcagc gatgagtctc gtcgtttggg
     2461 ccggcagacc gagacgctgc gttgcgcgca gaataacagg gcgccggtgg cgggcgaatg
     2521 agaagacaat cacgcggtca ttgtcgcgcg gagcgtagcg aaaagccgca tttttcgctc
     2581 tattagaaag agtttcttcg ccgcgcccag agtgacggat cggcggtgac agcccgataa
     2641 ggagacactg ctttgaaaca agggggtttc acctttgtcc tgcatagcca cctgcccttc
     2701 taccggcaga caggccgctg gccgcacggc gaggaaatgc tgcacgaggc gatggccgag
     2761 acctatatcc cgctgctcga cgccctgctc gacctggccg ccgagggcgt gccctaccgg
     2821 ctgacgctgg gcatcacgcc ggtgctggcc gagcaactgc gcgacccgct catcggcgag
     2881 cacttcgacc gctacatcgc ccacgaactg gccctggccg acagcgaccg cgaacgggcc
     2941 gagaaccgcg gcgacagcgc gggcgcggcc ctggcccgct attacgaagg gtattacacc
     3001 cgcgccgggc gcgtgttcaa cgagcgcctg ggccgcgata ttctgggcgc ggcccggcgc
     3061 ttgcaagacg cgggctatat cgagatcctc acctcggcgg ccacccacgc ctatctgccg
     3121 ctgctgtcgc gcgattccag tatccacgcc cagatcgcgg tgggcaagca gacctacgag
     3181 cggctctacg ggcgcgcgcc gcgggccatc tggctgcccg aatgcgccta ccgcccggcc
     3241 tacgtcaccc acagcgagag tcgtggcggt tatgtcaagc cgggcatcga gagcttcctg
     3301 gccgctgcgg ggatcggcct cttcttttgc gaaacccacg ccgtcaccgg gggcgacccg
     3361 gcgggcaaag ccggcggcgg cgccatcggc tacgcgccca tccccgaccg cctgctggtg
     3421 cccatcccca actacatccc gcccaccaac cgcacgacct acctgcccta cctggtgggc
     3481 cgcagcgacg tggccgtcat ggcgcgcaac gagcgcacgg ggcagcaggt ctgggcggcc
     3541 cagatcggct atccgggcga cccctggtac cgcgagttcc accgcaagga cctgaacagc
     3601 ggcatgcagt actggcggat caccggccac gaggtgccgc tgggcctgaa ggcgccctac
     3661 gacccggccc acgcccagca gcgcgtgcgc gagcacgccc accacttcac cggcctggtc
     3721 gaggatctgc tgcggcagta ccacgaggcc ggtggcgcgc cgggcctcat cgtgtcggcc
     3781 tacgataccg agttgttcgg ccactggtgg cacgagggcg tggactggct caaggccacc
     3841 ctgcgcaacc tggcggccag ccccgccgtg gaactgacga cggcgagcgc ctggctggcg
     3901 gcgcacccg
//
LOCUS       DASUYC010000935         3172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1772453, whole genome shotgun sequence.
ACCESSION   DASUYC010000935 DASUYC010000000
VERSION     DASUYC010000935.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1772453"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..1934)
                     /gene="aceK"
                     /locus_tag="VF276_15285"
     CDS             complement(135..1934)
                     /gene="aceK"
                     /locus_tag="VF276_15285"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HEX6608264.1"
                     /translation="MLSEGDLTGRDSAPLDLPAMDPAAGAVAEESAEAIRAAFAAYQS
                     RFQEITRQARRRFAQCDWHSLQADAARRLDLYNEHVRRTVAALAASLEVYQRDKPFWT
                     AVKACYSAVNAAREDRELAETFFNSVSMRLVAPRAIDPEIEYVDLEPLMLPPPLEKEI
                     YRVHPAGEPTTALVADLLAAYDLGIAYEDRDRDARLVAAAIDARLHDLGQDGRMQLIE
                     MARPIFYRGKGAYLVGRIATRNHYVPLVLVLLHGPGGVYVDAVLLTEDDVSIVFSFTR
                     AYFHVDTEYPAALVGFLKLIVPRKHVAEIYISIGYNKHGKTELYRDLLRHLAGSTDRF
                     EIAPGARGMVMTVFTLTSYDVVFKIIKDQFDYPKTTTRREVQSRYQLVFKHDRGGRLV
                     DAQEFEHLRFAKSRFAPDLLAELLNVAANTVSVDGEEVVIKHLYTERRLTPLDLYLRQ
                     VDPADATAVVMDYGQAIRDLAATNIFPGDLLLKNFGVTRHRRVVFYDYDELCLLTDCR
                     FREMPPPADPDEDMAAEPWFSVDENDVFPEELVTFLGLPPPLRAIFAPAHGDLFGVPF
                     WAQLQARLAAGEVIDILPYKPSHRLQPAAGGQG"
     gene            complement(1998..>3172)
                     /gene="aceA"
                     /locus_tag="VF276_15290"
     CDS             complement(1998..>3172)
                     /gene="aceA"
                     /locus_tag="VF276_15290"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HEX6608265.1"
                     /translation="SEEHTLAKRGSEKLWKQLTEEHKEGKYTNALGALTGNQAVQQVK
                     AGLQAIYLSGWQVAADANLAGQMYPDQSLYPANSVPAVVRRINQALQRADQIDHAEGR
                     NGTYWFAPIVADGEAGFGGPLNVFELTKAMIEAGAAGVHYEDQLASEKKCGHMGGKVL
                     IPTMHAIRHLVAARFAADVMGVSTVLLARTDANAATLLTSDIDPRDQPFLTGERTAEG
                     FYRIRNGIDTAIARGRAYAPFADLLWCETAEPSLPEARRFAEAIHAAYPGKLLAYNCS
                     PSFNWQKKLDAGTIAHFQRELGALGYRFQFVTLAGFHALNYGMFTLAHDYRERGMAAY
                     SELQQAEFAAEASGYTATKHQREVGTGYFDEVAQVIAGGESSTTALAGSTEREQFH"
BASE COUNT          512 a         1004 c         1100 g          556 t
ORIGIN      
        1 gcgcctgccg gccatcggtt gtgacctgcc gatggccgag gtttatcgca aggtcgtctt
       61 tcccgccagc gctgagacct ctggtcccag cgaaaccggc gaccagcaag ctccgtagcc
      121 accaagagcg aaaatcaccc ctggccgccg gctgccggtt ggagccggtg gctgggcttg
      181 taggggagga tgtcgatgac ctcgcccgcc gcgagccggg cctgcaactg cgcccagaac
      241 ggcaccccga acagatcgcc gtgggccggg gcgaagatcg cccgcaacgg cggcggcaag
      301 cccaggaagg tgaccagctc ctccgggaac acgtcgtttt cgtcgaccga gaaccagggt
      361 tcggcggcca tatcttcatc cgggtcggcg ggcgggggca tctcgcggaa gcgacagtcg
      421 gtgagcaggc agagctcgtc gtagtcgtag aacaccaccc ggcggtggcg ggtgacgccg
      481 aagttcttca agagcaggtc gccggggaag atattcgtgg ccgcgagatc cctgatggcc
      541 tggccgtaat ccattaccac cgccgtggcg tcggcggggt ccacctggcg caggtagaga
      601 tcgagcggcg tgagccggcg ctcggtgtag agatgcttga tcaccacctc ctcgccgtcc
      661 acgctgacag tattggccgc cacgttcagc aattcggcca gcaggtcggg cgcaaagcgg
      721 gatttggcga agcgcaggtg ctcgaattcc tgggcgtcca ccaaccggcc cccgcggtcg
      781 tgtttaaaca ccagttgata gcggctctgc acctcgcgac gggtggtcgt cttgggataa
      841 tcgaactggt ccttgatgat cttgaagacg acgtcgtagg aggtcagcgt gaacacggtc
      901 atgaccatgc cgcgcgcgcc gggggcgatc tcgaaacggt cggtcgagcc ggcgagatgg
      961 cgcagcaggt cacggtagag ttcggtcttg ccgtgcttgt tgtagccaat cgaaatgtag
     1021 atctcggcga cgtgcttgcg cggcacgatc aacttcagga agccgacgag cgccgcgggg
     1081 tattcggtat cgacatggaa gtaggcccgc gtgaagctga acacgatgct gacatcgtcc
     1141 tcggtcagca agacggcgtc cacgtagacc ccgcctggtc cgtgcaacag caccagcacc
     1201 aggggcacat aatggttgcg ggtcgcgatg cggcccacca gataggcccc tttgccccgg
     1261 tagaagatgg ggcgggccat ctcgataagc tgcatgcgcc cgtcctggcc caggtcgtgc
     1321 aagcgggcgt cgatcgcggc ggccactaac cgcgcatcgc gatcccggtc ctcataagcg
     1381 atgccgaggt cgtaggctgc cagcaggtcg gcgaccagcg cggtggtggg ctcgcccgcc
     1441 gggtggacgc ggtagatctc tttttcgagc gggggcggca gcatgagggg ttcgagatcg
     1501 acgtattcga tctccgggtc gatggcgcgg ggcgccacca ggcgcatcga caccgaatta
     1561 aagaaagtct cggccagttc gcggtcctcg cgtgctgcgt tgacggcgct gtagcaggct
     1621 ttgacggcgg tccagaaggg cttatcgcgc tggtagacct ccagggatgc cgccagcgcg
     1681 gctacggtcc gccgcacatg ctcgttatag aggtccagcc ggcgagcggc atcggcctgc
     1741 aggctatgcc aatcgcactg ggcaaagcgc cgccgggcct gccgggtgat ctcctggaag
     1801 cgtgactggt aggcggcaaa ggccgcgcga atggcctccg cgctctcttc cgcgaccgcc
     1861 ccggcggcgg ggtccatcgc gggcaggtct agcggcgcgg agtcgcgccc ggtcaggtcc
     1921 ccttccgaca acacacgggg tatcctcctg ccgccgcgcc ggatcagtcc cggcgcgggc
     1981 ggcctgctca ccgccaacta atgaaactgt tcccgctcgg tcgatccggc cagggccgtc
     2041 gtggacgact cgccgcccgc gatcacctgg gccacttcat cgaagtagcc ggtgccgact
     2101 tcgcgctggt gtttcgtcgc cgtgtagccg ctcgcctcgg cggcgaattc ggcctgctgc
     2161 aactcggaat aggccgccat gccgcgttcg cggtagtcgt gggccagcgt gaacatccca
     2221 tagttgagcg cgtggaaccc ggccagggtg acgaattgga accggtagcc cagcgcgccg
     2281 agttcgcgct ggaagtgggc gatcgtgccc gcgtcgagct tcttctgcca gttgaacgac
     2341 ggcgagcagt tataagccag gagcttgccg gggtatgcgg catggatcgc ttcggcgaag
     2401 cggcgggctt cgggcaggct tggttcggcg gtttcgcacc acagcagatc ggcgaacggc
     2461 gcgtaggccc gcccgcgtgc gatggccgtg tcgatgccgt tgcggatgcg gtagaagccc
     2521 tcggcggtgc gttcgccggt caggaacggc tggtcacggg ggtcgatgtc gctggtgagc
     2581 agcgtggcgg cgttggcgtc ggtgcgcgcc agcagcaccg tggacacgcc catgacatcg
     2641 gcagcaaagc gggccgccac caggtgccgg atggcgtgca tggtcgggat cagtactttg
     2701 ccgcccatat gcccgcactt cttctccgac gcaagctgat cctcgtagtg gacgcctgcg
     2761 gcgcccgcct cgatcatggc cttggtcagt tcaaagacgt tcagcgggcc gccaaacccg
     2821 gcctcgccgt cggcgacgat gggcgcgaac cagtaggtgc cgttccgccc ctcggcgtgg
     2881 tcgatctgat cggcgcgttg cagcgcctgg ttgatccggc gcaccacggc ggggacactg
     2941 ttggccggat acaggctctg gtccgggtac atctgcccgg ccaggttggc gtcggcggcc
     3001 acctgccagc cgctgagata gatcgcttgc agcccggctt tgacctgctg cacggcctgg
     3061 ttgccggtca gtgcgcccag ggcattcgtg tacttgcctt ccttgtgctc ctcggtgagc
     3121 tgcttccaca gcttctcgga gccgcgcttg gccagggtgt gctcttcgga ga
//
LOCUS       DASUYC010000936         5921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_412311,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000936 DASUYC010000000
VERSION     DASUYC010000936.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5921
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412311"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            332..814
                     /locus_tag="VF276_15295"
                     /pseudo
     CDS             332..814
                     /locus_tag="VF276_15295"
                     /inference="COORDINATES: protein motif:HMM:NF015216.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkylmercury lyase family protein"
     gene            complement(938..1912)
                     /locus_tag="VF276_15300"
     CDS             complement(938..1912)
                     /locus_tag="VF276_15300"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEX6608266.1"
                     /translation="MTKTMQTPVEFAAPAAPPEPDTLSWAEVRDWRTRRYRHQADLRL
                     RSEADARRFIDEVGFCFLFSAPGAELPTLWEAINGKPRAIPKHHHDHALSLTWSWKDS
                     LPARKEVWYGKLLRGKPMFVALDLLPAFYALSENYGELDDYRQQFADGRMSVEARAVY
                     EVLLEHGALSTNAMRKLSGLFGGGETARRFERAVAELQQDLKIVKCGTAEDNRWKYCY
                     VYDLLLRRYPDVAERARTITTRQARQQVVLRYLDNVAAAPFAGLQSLFGWEAETLRRT
                     VDELLATGRLREVAIPDLPAALNKPRSRKLKNAGAAPIWLALGPRHGL"
     gene            2075..2148
                     /locus_tag="VF276_15305"
     tRNA            2075..2148
                     /locus_tag="VF276_15305"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2109..2111,aa:Asp,seq:gtc)
     gene            complement(2355..2531)
                     /locus_tag="VF276_15310"
     CDS             complement(2355..2531)
                     /locus_tag="VF276_15310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608267.1"
                     /translation="MSARLPYPTWGGMIAAENLLRDVSINVIWPPAVCLTLISLAFVF
                     ISDGLRDGLDPREG"
     gene            complement(2567..3703)
                     /locus_tag="VF276_15315"
     CDS             complement(2567..3703)
                     /locus_tag="VF276_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX6608268.1"
                     /translation="MRKTFLYRINPTKAQATRLSKNLEECRWVYNQLLAQREAYWTEA
                     GIAVSMYAQHQYLPFLKEDRPSLRCVHSQVLQNVSERLDLAFQAFFRRVKSGENPGYP
                     RFRGYGRYDSITYPQYGNGAKISGSQLQLSKIGNVPIILHRPLEGTPKTVTIRRSSTG
                     KWYATISCEWEPTPLPGCPNQVGIDVGLASFATCSDDEVIDNPRFFRTEERALAKVQR
                     KHAKLEKGSPARRKHRKVVAGVHERIKLRRHNFTHQHSRKIVNHNGLIAVEDLSINKM
                     LHNHCLAKSISDAAWASFSQLLSVKAAWAGRKYIAVNPAYTSQTCSQCGHRQKIPLSE
                     RVFDCPCCCLHIDRDLNASLNILALGLQSIGTQSVEAPGFSHGE"
     gene            complement(3828..4601)
                     /locus_tag="VF276_15320"
     CDS             complement(3828..4601)
                     /locus_tag="VF276_15320"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6608269.1"
                     /translation="MELTASEAPIALVPHSSVWRGALMRLLHNKLAMAAIGVMLAFIL
                     VAAFAPQLAPHPYDYQSRARPDRGNGGLDLPPIWVQTGDPLTSGKPGFWLGTDSLGRD
                     TLSRVIYASRISIPTGFIPLLFTLPFGIGVGLAAGYLGGRVDQWLMRLTDTIFAIPQL
                     LLVVLVAFILRPTPLAEPLQGMVPVFLALALTGWVTLARLVRGQALTLKEEEFIVAAR
                     AVGMSPARIMFRYLLPNMAGPLITTAALSIPLFIESESG"
     gene            5002..>5921
                     /locus_tag="VF276_15325"
     CDS             5002..>5921
                     /locus_tag="VF276_15325"
                     /inference="COORDINATES: protein motif:HMM:NF039484.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX6608270.1"
                     /translation="MNPRLRIAGRAGAGLGLAALSGILITLAFPPYNLWPLIWMGFVP
                     GIVAQFRVLPSRASGLAYGVMIGGFFWGYFGPMFAGTVWFMEWLPLIVGVIATLLSNG
                     DRVFHERTGYRWLVLHGAVVWVGIEAMRGFLPVIGTGGFVAYALYDQPWLLQPVGLFS
                     IYGLSLLVMLVNYAVGAGVIALLDRRRSLQAPMTGISAGLAWRWGIGVAAVLLAWVAL
                     SLSLLGQAAAPGPSIRVAAVQPADSPSSELQLQKLIGQTRQAAAQGAQLVVWPEGALA
                     FDPQVSHTAQLRALVRETGVYLALGYGAHSA"
BASE COUNT         1047 a         1840 c         1937 g         1097 t
ORIGIN      
        1 ggggttacta taccgcactc ccgcgcataa tgcaacctcc gcgccgccta cgcgcgctcg
       61 gtctttcgcc cgagccggag tccgcactgt ataataagtg gagcggattc agcacgaaag
      121 gagcatgaat atgctgccaa ccgaagatgt cacgcttttc gataagcagg tacgtgccca
      181 ggtttatgcc cactttgtcc agctcgggca agcaccggca atgagtgagc ttgcccaggc
      241 gctggcgcgg cccgtgcccg atgtgcaagc cgcatatcag cgactcgccg cggggcgggc
      301 cctggtactc caggacaacg gcgaagtcct gatggcggag ccgttttcgg ccattcccac
      361 ggcctttgcg gtgcaagggc gcgggcacca gtggtggggc aactgcatct gggatgcgct
      421 gggcatcgcc gccatgctca gagcggacgc gcatattgcc acgtcgtgcg gatgttgcgg
      481 gtatgcgatg gcgctggacg tgcaagccgg cgcgttgcag ccggcgccgg gcgtcatcca
      541 ctacgcggtc ccgccgcgcg actggtggaa tgacgtggtg tttgcctgaa agacgatgct
      601 actcttccgg tcggaagaac acgtcgatcg ctggtgccgg cagtggcggc aaccgcacgg
      661 cgccgtcctc acgctggagc aacaatggcg cctggcgcag gcctggtatg cggatcggct
      721 cgactttgac tggcgccgca aaaccgcgga cgaagtggcg gcgctctggg ccgagttggg
      781 cttcgactcg ccgttctggg aactgccgcc gtaagcaaca gccgtagccg cgcggatgcc
      841 cgacgagccg gcggcgacgg taacttcgcg ctgtaggggc cggtttcaaa ccggcccagc
      901 tcctcgtcag gaccgccggc gtggcctacc ggcgcgctca gaggccgtgc cgcggcccga
      961 gcgccagcca gatcggcgcg gccccggcgt tcttcaactt gcggctgcgc ggcttgttga
     1021 gcgcggcggg cagatcgggg atggccactt cgcgcaggcg gcccgtcgcc aggagttcgt
     1081 ccactgtgcg gcgcagcgtc tcggcctccc agccgaacag cgattgcagc ccggcgaacg
     1141 gcgccgcggc cacgttgtcc aggtagcgca gcaccacctg ctgccgcgcc tggcgggtgg
     1201 tgatggtccg tgcccgctcg gccacgtcgg gatagcggcg cagcagcaga tcgtagacgt
     1261 agcagtactt ccagcggttg tcctcggcgg tgccgcactt gacgatcttc aggtcctgct
     1321 gcagttcggc taccgcgcgc tcgaagcgcc gggcggtctc cccgccgccg aacaggcccg
     1381 acagcttgcg catggcgttt gtggacagcg caccgtgctc cagcaacacc tcgtacaccg
     1441 ccctggcttc cacgctcatg cgcccgtcgg cgaattgctg gcggtagtcg tcgagctcgc
     1501 cgtagttctc ggagagcgcg tagaaggcgg gcagcaggtc tagcgcgaca aacatgggct
     1561 tgccgcgcag cagcttgccg taccagacct ccttgcgcgc gggcaggctg tctttccaac
     1621 tccaggtcag gctcagcgcg tggtcgtggt ggtgcttggg aatggcgcgg ggcttgccgt
     1681 tgatcgcctc ccagagggtg ggcaactccg cgccgggcgc cgagaagagg aagcaaaagc
     1741 cgacctcgtc gatgaagcgg cgggcgtcgg cctcggagcg caggcgcagg tcggcctggt
     1801 gacgatagcg gcgcgtgcgc cagtcgcgca cctcggccca cgagagggta tccggctccg
     1861 gcggggcggc aggggcggca aactctacag gcgtctgcat tgtcttggtc atagggataa
     1921 cggcatcctt tgctgggtgc cgggcggcgg tttcccgccc cggtcgcagg cgaacatata
     1981 tgcgactatt gtaccgaaaa acaccctggc acggcaaccg gccaaccttg acactggcga
     2041 gtaaatatga tatgatactg ccgcaaccgt ccctggaggc gtggtgtagc ggcctaacat
     2101 gcagccctgt caaggctgag atcgcgggtt cgaatcccgt cgcttccgcc ccattaaccc
     2161 cgaccgtatt ccggtcgggg tttttgtttg cccgcccgtg cgcaccgagt ggtggtggac
     2221 gggggcctgc tgctgaagta gctcacccac cgcagacaga caaacagcgc ccggatcgcc
     2281 gtgaaaagct gatccgggcg ccgtgtttat ggcggcgatc cccgctacgg cggggggcgg
     2341 cgcggggccg gtggttagcc ctcgcgcggg tcgagcccgt cgcgcaaccc gtcgctgatg
     2401 aacacgaacg ccagcgagat gagggtcagg cacaccgccg ggggccagat cacgttgatc
     2461 gacacgtcgc ggagcagatt ctccgcggcg atcatgccgc cccaggtggg atacggcagc
     2521 cgggcgctca cgcccatgta ctccaggatg atctccgtga cggtgattac tccccgtggc
     2581 taaagccagg ggcttctacg gattgagtac cgatgctctg taatcccaga gcaagaatat
     2641 tcaaactagc gttcaggtcg cgatctatat gcaaacagca gcacggacaa tcgaataccc
     2701 gttcagacaa cgggattttc tgtcggtgcc cacattggct gcatgtctga gaagtgtagg
     2761 ccgggtttac agcgatgtat ttccgcccgg cccatgctgc cttgacggaa agcaattgac
     2821 tgaaactggc ccacgccgcg tcgctgatgc ttttggcaag acagtggttg tgcagcattt
     2881 tgttgatgct gaggtcttcc acagcgatca gtccgttgtg atttactatt tttcggctgt
     2941 gctggtgcgt gaagttatgg cgccgcaact taatccgctc atggacgcca gcaacgactt
     3001 tacggtgctt gcgccgagcc ggactaccct tttccaattt cgcgtgcttg cgttgtacct
     3061 ttgccagcgc ccgttcctcg gtgcggaaaa agcgcggatt gtcgatcact tcgtcatcgg
     3121 agcaagtagc aaaactggca agcccaacat caatgcccac ctgattaggg caccccggca
     3181 acggggtcgg ttcccattcg caagaaatgg ttgcatacca cttaccagtg gaagagcgcc
     3241 gaattgtgac agtcttgggc gtcccctcca gcggacggtg caaaatgatc ggtacattgc
     3301 caattttgct caattgcagt tggctaccag agattttggc cccgtttccg tactgcgggt
     3361 aagtaatgct atcgtagcga ccgtatccac gaaagcgagg gtagcccgga ttttcaccgg
     3421 atttgactcg gcggaagaat gcctggaatg ctaggtcgag ccgttcagat acgttctgca
     3481 acacttggct atgcacacac ctaaggctag gacggtcttc tttgagaaac ggcaaatact
     3541 gatgttgcgc gtacatactg acggcaatac cggcttcagt ccagtaagcc tcacgttgcg
     3601 ccagaagctg gttataaacc catcggcatt cttccaggtt tttgcttagc cgggtagcct
     3661 gtgctttagt cggattaatc cgatacagaa atgtcttgcg cataccggaa ttttactaca
     3721 cttacatgtg tgttacaatt accggtacca gcacaaatct tgctactggc tccgatcaca
     3781 cctactgcgt aggggcggct gtatccccat tgctgaagca aggggtctta gccgctttcg
     3841 gattcgataa acagggggat ggacaacgcc gccgtcgtga tcagcgggcc ggccatattg
     3901 ggcagcaggt agcggaacat gatccgcgcg ggcgacatcc ccacggcgcg cgcggccacg
     3961 atgaactcct cctctttcag ggtcagcgcc tgcccgcgca ccagccgggc cagtgtaacc
     4021 cacccggtca gcgccagggc caggaagacc ggcaccatgc cctgcagcgg ttcggcgagc
     4081 ggcgtggggc gcagaatgaa cgccaccagg accaccagca ggagttgcgg gatcgcgaat
     4141 atcgtatcgg tcaggcgcat cagccactgg tcgacccgac ctcccaggta gccggccgcc
     4201 aggcccaccc cgatcccgaa cggcagggta aacagcagcg gaatgaaccc ggtggggatg
     4261 gaaatgcgcg aggcgtagat cacccggctc aaggtgtcgc ggcccaggct gtcggtgccc
     4321 aaccagaagc cgggcttgcc gctggtcagc ggatcgccgg tctggaccca gatcggcggc
     4381 aagtccaggc cgccgttgcc ccggtcgggg cgggcgcggc tctggtagtc gtacggatgc
     4441 ggggccagtt gcggggcaaa cgcggcgacg aggataaagg ccagcatcac gccgatggcc
     4501 gccatcgcta acttattgtg caagagccgc atgagggcgc cgcgccacac gctgctatgc
     4561 ggcaccaggg ctattggcgc ctcggaagcc gtcagttcca tagacctccg tgggggatcg
     4621 atgcgcccgg tggtccgcgg cgcctgctgt gcgtagaagt cacccttata gtacaggtgg
     4681 gccatgcaca cggttcagca cgcgggggca agcgggccgc catatcggca accaaaggcg
     4741 ataccgcttg ctatcggccc ctggccggca gatcggctac ggcggcccct ctgatccggg
     4801 gtcaggcgcc ccagcgaaac cgggcgccac cggatgataa gagatcttgc cacaaaattg
     4861 caacctcccg caacctctcc cacaactctc ggttgctaga atcacctggg cgaattccat
     4921 gccggggcgc ccgctcccgt ccgcgcacgc gttgttcgca tcctggccta ccggcagcag
     4981 tgaggaaagg cgagggaacc tatgaatcca cggctacgca tcgccggccg cgctggggcc
     5041 gggttgggtc tggcggcgct gagcggcatc ctgatcacgc tggcatttcc accctataat
     5101 ctctggccgc tcatctggat ggggttcgtg ccgggcattg tcgcacaatt ccgggtgctg
     5161 ccctcgcggg cgagcggcct ggcctacggc gtcatgatcg gcggcttctt ctggggttac
     5221 ttcgggccga tgtttgccgg cacggtctgg tttatggagt ggctgccgtt gatcgtcggg
     5281 gtcatcgcca cactgctaag caacggggat cgcgtgttcc acgagcgcac gggctatcgc
     5341 tggctggtgc tgcacggcgc cgtggtctgg gtggggatcg aggcaatgcg cggcttcctg
     5401 ccggtcatcg gcaccggcgg ctttgtggcc tacgcgctgt atgaccagcc gtggttgctg
     5461 caaccggtgg gcctgttcag catctacggt ctgagcctgc tggtcatgct ggtaaactat
     5521 gcggtgggcg ccggtgtgat cgccctgctg gaccggcgcc ggtcgctcca ggcgcccatg
     5581 accggcataa gcgccgggct cgcatggcgt tgggggatcg gcgtggcggc tgttctgctg
     5641 gcctgggtgg cgctcagcct gagcctgctg ggccaggccg ccgcccccgg cccgagtatc
     5701 cgtgtggcgg cggtgcagcc ggcagacagc cccagcagtg agcttcagct acaaaagctc
     5761 atcggacaaa cccggcaagc cgcggcgcag ggcgcgcaac tggtggtctg gccggaaggc
     5821 gcgctggcct ttgacccgca ggtgagccac accgcgcaat tgcgcgccct ggtgcgcgag
     5881 accggcgtgt acctggccct gggctacgga gcgcacagcg c
//
LOCUS       DASUYC010000937         3437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_2004229, whole genome shotgun sequence.
ACCESSION   DASUYC010000937 DASUYC010000000
VERSION     DASUYC010000937.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2004229"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="VF276_15330"
     CDS             <1..944
                     /locus_tag="VF276_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608271.1"
                     /translation="IPPGTARFTVRLTPGDGLPADDQAVLLLAAAGQHPVHVQSPAPD
                     LYLRALAGLPGLAPSTDAPANAAAELTIIEGQLPDPLPGGSLLLVNPTGALLPATGTL
                     ANVRPTATGLESPLLAGVDLQALQVRKAQQIGSVPWLDPVMVAGGGPLLLAGTRDGRR
                     IAVLTFDPRDSNLPSLAAFPLLLANLVEWLDPLAGVQALHPGDPVPLAPAAMVTAPDG
                     ATARVGERGVYAGTEAAGLYRVTGGAEPARTFAVNMTDAGESALAPRPHPELQRDAPL
                     AAPESQTEQEFWPLLAALAAGLLGAEWILYCWKRGRL"
     gene            941..>3437
                     /locus_tag="VF276_15335"
     CDS             941..>3437
                     /locus_tag="VF276_15335"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX6608272.1"
                     /translation="MSNIVFLRPEVLWLALGIPLLLLAGRRLAGLTRLRRGLVIAVEG
                     VAMLLLLAALAQPVLPKPDQDMNLVLVLDSSASLSPASRAQARTYAQDVLASAGPTDH
                     VRVVAAGSTARLLNPDDLAGDAWQESAADGGAAGGGTDLAAGLRLAGSLLPDSGRRRV
                     VLLSDGWETQGRAADEAARLQARGIDLNVVALQALGDPEVVAQGLALPAYARVGDALS
                     SDLTVLSTRATSATVQVRVDGQVQGRQSVTLPAGTSHITLDQQARTPGFHRVDVTVNT
                     AADSTTANNTTSASVVVKPAPHVLVLEDRTGEAANLASALTARGMTVDVGSPAQVPAE
                     AGGLDQYDSVILNNVAATSLTLDQQRTVQEYVRRHGGGLVTVGGRTSYARGGYADSVF
                     EEVLPVSSQPPPRPAQGATALILIIDRSYSMNDRSVFDDGVTKLAMARQAAQLAVDAL
                     REGDILGVLAFDMENIWALPPGPIQSAADKERAKRLISEIRPGGGTSIYPAVEEAAHT
                     IETITAPTKHLVLLTDGREFGYQDYGPVLSELQKADVSLSTIGIGSDTDQELLTQLAK
                     LGQGRYYFTERPQNIPKIVFKELDLAMKEPMVEGAVQPQLLVPSPVLRGFAPQDLPQL
                     RGYERTTAKDTATVGLVTDQGEPLLAQWSYGLGRVVAFTSDAGPGWAGPWLLWDEYAR
                     FWDQAVRWSMTSPVEPQLQPAITVSRPAGAATGYGVAHLTVESLHADNSFADLANLTV
                     AVRAPSGAVTTTVLAQTAPGRYEGDLPLAEPGAYEARFTRQENGATVSETAGFSVPPG
                     PELLHAGTNDALLQTLAAAKPYLTTAAGALDRQG"
BASE COUNT          492 a         1249 c         1244 g          452 t
ORIGIN      
        1 agatcccgcc cggcacggcc cgcttcaccg tgcgtctgac ccccggcgac ggcctgcccg
       61 ccgacgacca ggccgtcttg ctgctggccg ccgccgggca gcacccggtc catgtgcaga
      121 gcccggcgcc cgatctctac ctgcgcgcgc tggccgggct gccgggcctc gcgcccagca
      181 cggacgcacc ggcgaacgcc gccgccgagt tgacgatcat cgaaggccaa ctgcccgacc
      241 cgctgcccgg cggcagcctg ctgctggtca accccaccgg cgcgctcttg ccggccaccg
      301 gcacgctggc gaacgtgcgc ccgaccgcta ccggcctcga aagcccgctg ctggcggggg
      361 tggatttgca ggcgttgcag gtgcgcaagg cgcagcagat cgggtccgtg ccctggctcg
      421 acccggtgat ggtggccggg ggcgggccgc tgctgctggc cgggacgcgc gacgggcgcc
      481 gcatcgccgt gctgaccttc gatccgcgcg actccaacct gccgagcctg gccgccttcc
      541 cgctgctgct ggcgaacctc gtcgagtggc tcgacccgct ggcgggcgtg caggcgctgc
      601 accccggcga cccggtgccg ctggcgcccg ccgccatggt cacggcgccc gacggcgcga
      661 cggcccgcgt gggcgagcgc ggcgtgtacg cgggcaccga ggccgccggg ttgtaccggg
      721 tgacgggcgg cgcggaaccg gcccgcacat tcgccgtcaa catgaccgat gccggcgagt
      781 ccgccctggc gccgcggccc catcccgaat tgcagcgtga cgcgcccctt gccgcgcccg
      841 aatcgcagac tgagcaggag ttctggccgc tgctggcggc gctggccgcc gggctactcg
      901 gggcagaatg gatcctgtac tgctggaaac gaggccgcct gtgagcaaca tcgtcttctt
      961 gcgtcccgaa gtgctctggc tggcgctggg catcccgctg ttgctgctgg ccgggcggcg
     1021 gttggccggg ctgacgcgtc tgcggcgcgg gctggtgatt gcggtggaag gcgtcgccat
     1081 gctcttgctg ctggccgcgc tggcccagcc ggtgctgccc aagcccgacc aggacatgaa
     1141 cctggtgctg gtgctggata gctcggcgag tctcagcccg gcgagccgcg cgcaggcgcg
     1201 cacctacgcc caggatgtgc tggcgagcgc cggtcccacc gaccacgtgc gcgtggtggc
     1261 ggcgggcagc acggcccggc tgctcaatcc cgacgacctg gccggcgatg cctggcagga
     1321 gagcgcggcg gatggcggcg cggcgggcgg cgggacggac ctcgccgcgg gcctgcgcct
     1381 ggccgggagc ctgctgcccg acagcgggcg gcggcgcgtg gtcctgctga gcgacgggtg
     1441 ggagacccag ggccgcgccg ccgacgaagc ggcgcgcctc caggcccgcg gcatcgacct
     1501 gaatgtggtg gcgctgcaag cgctgggcga tcccgaagtg gtggcccagg ggctggcgct
     1561 gccggcctac gcgcgggtcg gcgacgcgct gtccagcgac ctgactgtgc tcagcacgcg
     1621 cgccacctcg gccacggtgc aggtgcgcgt ggacggccag gtccagggcc ggcagagcgt
     1681 gacgctgccc gcgggcacga gccatatcac cctcgaccag caggcgcgca cgcccggctt
     1741 ccatcgcgtc gatgtgacgg tgaacaccgc cgcggattcc acgacggcca ataacacgac
     1801 cagcgcatcg gtagtggtca aaccggcccc gcacgtcctc gtgctggaag accgcacggg
     1861 cgaggcggcg aacctggcga gcgcgctgac cgcgcggggg atgaccgtgg atgtgggcag
     1921 cccggcccag gtgcccgccg aagccggcgg ccttgaccaa tacgacagcg ttatcctgaa
     1981 caacgtcgcg gcgacgagcc tgacgctcga ccagcagcgc accgtgcagg agtatgtgcg
     2041 gcggcacggc ggcggcctgg tcacggtcgg cgggcgcacc agctatgcgc gcggcggcta
     2101 cgccgacagc gtgttcgaag aagtgctgcc cgtttcgtcg cagccgccgc cccgcccggc
     2161 ccagggagcg acggcgctga tcctcatcat cgatcggtcg tatagtatga acgaccgcag
     2221 cgtcttcgac gacggcgtga ccaagctcgc catggcccgc caggccgcgc aactggccgt
     2281 ggacgcgctg cgcgagggcg acatcctggg cgtgctggct ttcgacatgg agaatatctg
     2341 ggccttgccc cccggcccca tccagtcggc ggccgacaag gagcgtgcca agcggctgat
     2401 ctccgagatc cggcccggcg gcggcacctc catctacccc gcggtcgaag aggccgcgca
     2461 caccatcgag accatcaccg cgcccaccaa gcacctggtg ctgctcaccg acgggcgcga
     2521 gttcggttac caagactacg gccccgtgtt gagcgagctg caaaaggccg acgtgagcct
     2581 atcgaccatc ggcatcggca gcgacaccga ccaggagttg ctgacgcaac tggcgaagct
     2641 gggccagggc cgctactact tcaccgagcg gccccagaat atccccaaga tcgtgttcaa
     2701 ggaactagac ctggcgatga aggagccgat ggtcgagggc gcggtgcagc cgcaactgct
     2761 ggtgcccagc cccgtgctgc gcggcttcgc gccgcaggac ctgccgcaac tgcgcggcta
     2821 tgagcgcacc actgccaaag atacggccac ggtaggcctg gtcacggacc agggcgagcc
     2881 gctgctggcc cagtggagct acgggttggg gcgcgtggtc gccttcacgt ccgatgccgg
     2941 gccgggctgg gccggtccct ggctgctgtg ggacgagtac gcgcgcttct gggaccaggc
     3001 ggtgcgctgg tcgatgacca gcccggtcga gccgcagttg cagcctgcta tcaccgtgag
     3061 ccgtccggcg ggcgcggcga ccggctacgg cgtggcccac ctgaccgtgg aaagcctcca
     3121 tgccgacaac agcttcgccg atctcgccaa cctcacggtg gcggtgcgcg cgccgtcggg
     3181 cgccgtgacc accaccgtgc tggcccagac ggcgccgggc cgctacgaag gcgacctgcc
     3241 gctggccgag cccggcgcct atgaggcgcg cttcacccgc caggagaacg gcgccacggt
     3301 gagcgagacg gcgggcttca gcgtaccgcc cggccccgaa ctgctgcacg ccggcaccaa
     3361 cgacgcgctg ctgcagacgc tggccgcggc caagccgtac ctcactaccg cggcgggcgc
     3421 gctggaccgg caggggc
//
LOCUS       DASUYC010000938         2785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1417577, whole genome shotgun sequence.
ACCESSION   DASUYC010000938 DASUYC010000000
VERSION     DASUYC010000938.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1417577"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1435
                     /locus_tag="VF276_15340"
     CDS             <1..1435
                     /locus_tag="VF276_15340"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX6608273.1"
                     /translation="QAAAAASGQPPLLIGADQEGGTLMALAGTTAFPGNMALGAAGDP
                     ALARQVGFALGQELAAQGVNVNYAPVCDVNNNPTNPVVGTRSFGEDPAAVAALAAAMV
                     TGLQDAGVAATLKHFPGHGDTSGDSHHGLPVVAHDAARLARIELPPFAAGIAAGARLV
                     MTAHIALPAIDAAPDLPATLSPAILRGILRDRLGFDGVIISDALDMRAIEQGAGLVID
                     AIAAAAAGVDLLILNSVVEDQEAVAAGMRQAVARGLLPSEATLASAGRVLDLKRWVSA
                     QPQPSLDVVNSPAHRALALEVAARAVTLVRDDAGLLPLRPAPEARLAAVVPQPADLTP
                     ADTSSYVTPSLAAALRRRHPHVDEFIVPLNPTAADVAALCDQLRGYDLVVIGTINATA
                     HAGQAALVQALLDGGPPVVAVALRLPYDLAAYPAAPTYLCTYSLLESSMEALSDVLWG
                     ARPVGGRLPVSIPGLYPLGHGRSIGAG"
     gene            1546..2739
                     /locus_tag="VF276_15345"
     CDS             1546..2739
                     /locus_tag="VF276_15345"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6608274.1"
                     /translation="MPEELELSLMTDTAAPIQPLLDQFEAAHGIRVRPRLLSWDAGWN
                     TLFQSALQGRGSDVSEIGSTWLGDLAGMNALQAFSAEAVAEMGGAEAFMPVAWRSCQM
                     AGQPQTWAIPWLVGARLLFYRRDLLARHGIDPQTAFGTADRLDRTLGQLQAAGVSVPW
                     TVPTGSTHTTLLNVASWVWGAGGAFVTPDGKRTQINQTAARAGLRAYFALGRYLAPSV
                     RRLSGTQPDTVFLQNPDAAMTISGSWLFQRAPDQGSPDLAVQLGAALPPGPSFVGGSH
                     LVIWKHSPNYAAAVQLVRFLTSAPALIPYSKAIGLLPATLDALDAEPFAGDPLWQQTR
                     EGVRTGRSFPVIRAWGMIEYRLVAALSSLWAETLASPGQDVDAILRKHLDPLAQRLDQ
                     LLGQS"
BASE COUNT          382 a          982 c         1011 g          410 t
ORIGIN      
        1 gcaagccgcc gcagcggcga gcggccagcc acccctgctc atcggcgccg accaggaggg
       61 gggcacgctg atggccctgg ccggcaccac ggcgttcccc ggcaacatgg cgctgggcgc
      121 ggcgggcgat cccgccctgg cccggcaggt gggcttcgcg ctgggccagg aactggcggc
      181 gcagggcgtg aacgtcaact acgcgccggt gtgcgatgtg aacaacaacc cgaccaaccc
      241 ggtggtgggc acgcgctcgt tcggcgaaga cccggcggcg gtggccgcgc tggcggcggc
      301 catggtcacg ggcctgcaag acgccggggt ggcggccacg ctcaagcact tccccggcca
      361 cggcgacacc agcggcgact cgcaccacgg gctgccggtg gtggcgcacg acgcggcccg
      421 gctggcgcgc atcgaactgc cgccgttcgc ggcgggcatc gcggcgggcg cccggctggt
      481 catgaccgcg catatcgcgc tgccggccat cgacgccgcc ccggacctcc cggccacgct
      541 ctcgccggcc atcctgcgcg gcatcctgcg cgaccggctg ggcttcgacg gcgtgatcat
      601 cagcgatgcg ctggatatgc gggctatcga gcagggcgcg ggcctggtga tcgacgccat
      661 tgccgccgcc gccgcgggcg tggacctgct gatcctcaac tcggtggtcg aagaccagga
      721 agcggtggcc gcggggatgc gccaggcggt ggcgcgtggc ctgctgccaa gcgaggcgac
      781 gctggcctcg gcggggcgcg tgctcgacct caagcgctgg gtgagcgcgc agccgcagcc
      841 gtcgctcgac gtggtgaaca gcccggcgca ccgggcgctg gcactggaag tggcggcccg
      901 cgcggtgacg ctggtgcgcg acgacgcggg gctgctgccg ttgcgacccg cgccggaggc
      961 ccggctggcg gcggtcgtgc cccagcccgc cgacctgacc cccgccgaca cctcgtccta
     1021 tgtgacgccc agcctggccg ccgcgctgcg ccgccggcac ccgcatgtgg acgagttcat
     1081 cgtgccgctc aacccgacgg ctgccgacgt ggccgcgttg tgcgaccaac tgcgcgggta
     1141 cgacctggtg gtgatcggca cgatcaacgc caccgcgcac gccgggcagg cggcgctggt
     1201 ccaggcgctg ttggacggcg ggccgccggt ggtggccgtg gcgctgcgcc tgccctacga
     1261 cctggcggcc tatccggcgg cgccgaccta tctctgcacc tatagcctgc tcgaatcctc
     1321 aatggaagcg ctcagcgacg tgctgtgggg cgcgcggcca gttggcggtc gcctgcccgt
     1381 gtcgatcccc ggcctgtacc cgctcggcca cggccggtcc ataggcgccg gctgatcgcg
     1441 ggttactatt tggggccggg caacgttgta gagagtgcga catttcactc cctgtagccg
     1501 gcgcgacagt gcggtgcgtc cgggaaccgg agaatgaggc ccatcatgcc ggaagaactc
     1561 gaactctcgc tgatgacgga cacggcggca cccatccagc cgttactcga ccagtttgaa
     1621 gccgcgcacg gcatccgcgt gcgaccccgg ctgctctcct gggatgccgg gtggaatacc
     1681 ctgtttcaga gcgccctgca gggccgggga tcggatgtgt ccgagatcgg cagcacctgg
     1741 ctaggcgacc tggccggtat gaacgctttg caggcgttta gcgccgaggc cgtcgccgag
     1801 atgggcggcg cggaggcgtt catgccggtg gcgtggcgca gttgccagat ggccgggcag
     1861 ccgcaaacct gggccatccc ctggctggtg ggcgcacggc tgctgttcta ccggcgcgat
     1921 ctgctggcgc ggcacggcat cgacccgcag acggcattcg gcaccgccga ccggctggat
     1981 cgcacgctgg ggcagttgca ggccgcgggg gtcagcgtgc cctggaccgt gcccaccggc
     2041 tcgacgcata cgacgctgct caatgtggca agctgggtgt ggggcgcggg cggcgcgttt
     2101 gtcacgcccg acggcaagcg cacgcagatc aaccagacgg cagcgcgcgc cgggctgcgt
     2161 gcctatttcg ccctgggtcg ctacctggcg cccagtgtgc gccggctcag cggcacgcaa
     2221 ccagacaccg tatttttgca gaatcccgac gccgccatga ccatcagcgg gtcgtggctg
     2281 tttcagcggg cacccgacca gggcagcccc gacctggcgg tgcaactcgg cgcggccctg
     2341 ccgcctggcc cgtcgttcgt cggcggctcg cacctggtga tctggaagca cagccccaac
     2401 tatgccgccg ccgtgcaact ggtgcggttc ctgactagcg ccccggcgct gattccctat
     2461 agcaaggcca ttggcctgct gccggccact ctggacgccc tcgacgccga gccgttcgcc
     2521 ggcgatccgc tctggcagca gacgcgcgag ggcgtgcgca ccgggcgctc gttcccggtg
     2581 atccgcgcgt ggggcatgat cgaataccgg ctggtggcgg cgttgagttc gctgtgggcc
     2641 gagacgctgg cgagtcccgg ccaggatgtc gatgcgatct tacgcaagca tctcgacccg
     2701 ctcgcgcagc gcctggatca gttgttgggc caaagttaat cgccgggcgg cgcgggccgg
     2761 tttgaaaccg gcccctacaa cgcaa
//
LOCUS       DASUYC010000939         5570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1310331, whole genome shotgun sequence.
ACCESSION   DASUYC010000939 DASUYC010000000
VERSION     DASUYC010000939.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5570
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310331"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..1372)
                     /locus_tag="VF276_15350"
     CDS             complement(131..1372)
                     /locus_tag="VF276_15350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6608275.1"
                     /translation="MLSVHTCPLATLGGKETGGMNVYVRELARELGRRGKQVDIFTRA
                     VDPHIAPVRQLGPNARVIHIPAGPVAPYDKNAIYCHLPEFVQRVEAFRVQEGIQYDLV
                     HAHYWLSGVAGHDLATRWGVPMIQMFHTLGHMKNEVAKHERDREVGLRIAMETRLVAQ
                     ADRIVSPTSTERAQLAWYYGADPERVTVIPCGVDTRLFRPRDQHAARQRLNLPGGRLL
                     LFVGRIERLKGIDTLLEAASLLAARPGYDDLRVLIVGGDLESNGDNPELRRVQRLAEE
                     LCINDRVDFLGSQPQTELPHFYAAADLTIMPSHYESFGMVALESMACGTPVVASRVGG
                     LARLVKDGETGILVPEEEPTALAMQIHATLQHDCEREAMGAQAAAYARDFAWGRIADR
                     ICDLYDEQIHSHANRECPCVV"
     gene            complement(1392..2567)
                     /locus_tag="VF276_15355"
     CDS             complement(1392..2567)
                     /locus_tag="VF276_15355"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6608276.1"
                     /translation="MSFEIECVGEFAAGPAPDEWDRVLAGSAADPLFLSRPFQEAWWC
                     TFCCNASASCQLALLLLREGGTLVGVAPFYLSTMDEAGWAEETSRAADWRRIAARAAA
                     QGDPAAERPEGRTAAGEAAATTAEPPEAAPRPGERVVRLVGGIAVADYLNIIAPAGRE
                     CEAWAAVLAYWAGRAADWDVLDLHSLPVASATSARCAASAHGTVWCGVEETCPVLALP
                     ATWDDYLAALGKKDRHELRRKLRRAESHPEPLTWRTVNKGPELAAALETFIALHRRSD
                     EAKARFMTPIMVTFFHRLLAALAPTGWLEVATLYAGDQPVAAYLSFRQAARLLLYNSG
                     YDPRYGGYSAGFALLVYRIRQAIEEGVQTFDFLRGDERYKYDLGAQDQFIYRVIIRK"
     gene            2790..3167
                     /locus_tag="VF276_15360"
     CDS             2790..3167
                     /locus_tag="VF276_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017719330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6608277.1"
                     /translation="MPRILVVEDNAMIREMMARRLQRRGYEILVAADGAEGVARAQVD
                     QPDLILMDMSLPVLDGWAATRRLKAAPATQAIPVIALTAHAMAGDRESCLAAGCDEYD
                     TKPVDFARLIGKIQALLDRAAAS"
     gene            3410..>5570
                     /locus_tag="VF276_15365"
     CDS             3410..>5570
                     /locus_tag="VF276_15365"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX6608278.1"
                     /translation="MVEPRGSTRRFAARLDPRYSFSARLGLALGLVAFVTSILISLAA
                     GYNSSAQITADSGQFLAEVAFHMSSQLDTSIQERFRDLQVLATLDTIRDPAVPRDTKR
                     DLLERLQNTYTDYSWMGLADSQGRIVVSTRRLLEGNDSLQRKWFVNGQQAPYLGQADQ
                     GLLLPNPAPDPTGEPLRFIDLAVPVSDLNGQPAGVLTAHLSWAWGQKIQAGLLEPLQT
                     RQHVEMLVLTHEGTVLLGPSEFKSQAQTLTLDSVAAARAGHNAYKTETWPDGKLFLTG
                     YAGIAGDRTNPRVPWIVLVRQSVTAAQAPARALQQEDLIRGILLGILFAVLSGLLAGR
                     LVRPLRGIAGAADRIRQGDAAATIPVHQGHDEVAVLSDSLSGLVSSLNGQKAELSTLN
                     AQLERELAERRQAEDDLRHLRDELEIRIQRRTADLVHANHELQTEVAERRRAEAALQW
                     SNALLVAEQEAAIDGILVTDAERRTVSVNGPFCAMWGLAPETARKLGPAALVESVLPL
                     LQRPEEFLADLSALYMPEHLKDTRRAEIGLKDGRIIDRYVAPVLSPEGEVWGRIWYFR
                     DVTRQRHATQRLAGQHAVARILTESNALSAALPRVVQAVLDTLGWDTGVIWMVDPSGA
                     ALRSRGVVAHGPDAPALPAAALEQLQPASGAGLPGQVWASGQPAWVADLAEQRDTPDA
                     ALLADAGLRGACALPVMQGEAVLGVLEFCSRDPQMPGA"
BASE COUNT          882 a         1960 c         1914 g          814 t
ORIGIN      
        1 gggcggaacg tgaaatccgc cgagccgatg ggatcgtcga gcacacgctc cgaacagcag
       61 atcacgtcca ccgggtgcca gagggtgtgg tcgccgtcga gcacgaccag ccggatcgga
      121 accgcgggcg ctacaccacg cacgggcact cccggttggc gtggctgtgg atctgctcgt
      181 cgtaaagatc gcagatgcgg tcggcgatgc ggccccaggc aaagtcgcgg gcgtaggccg
      241 cggcctgcgc gcccatcgcc tcgcgctcgc aatcgtgctg caacgtggcg tggatctgca
      301 tcgccagcgc ggtcggctcc tcttcgggca ccaggatgcc cgtttcgccg tccttgacca
      361 gccgggctag cccgcccacg cgcgaggcga ccaccggcgt gccgcaggcc atcgactcca
      421 gtgccaccat gccgaacgat tcgtagtgcg agggcatgat cgtcaggtcg gcggcggcgt
      481 aaaaatgcgg cagttcggtc tgcggctgcg agccgaggaa atcaacccgg tcgttgatgc
      541 acaactcctc ggccagccgc tgcacccggc gcagttccgg gttatcgccg ttgctctcca
      601 ggtcgccgcc gacgatgagc acgcgcaggt cgtcgtagcc ggggcgcgcc gccagcagcg
      661 aggcggcttc gagcagcgtg tcgatgcctt tgagccgctc gatgcgcccg acgaagagca
      721 gcaggcgccc gcccggcagg ttgagccgct gccgcgcggc gtgctggtcg cgcgggcgga
      781 acaggcgcgt atcgaccccg caggggatca ccgtgacccg ctccgggtcg gcgccgtagt
      841 accaggccag ttgggcgcgc tcggtcgacg tgggcgagac gatgcggtcg gcctgggcca
      901 ccaggcgcgt ttccatcgcg atgcgcaggc ccacttcccg gtcgcgctcg tgcttggcga
      961 cctcgttttt catatggccc agcgtatgga acatctggat catcggcacg ccccagcgcg
     1021 tcgccaggtc gtggcccgcc acgccggaga gccagtagtg ggcatgcacg aggtcgtact
     1081 ggatgccctc ctgcacgcgg aacgcctcca cccgctgcac aaactccggc aggtggcagt
     1141 agatggcgtt tttatcgtag ggcgccaccg gccccgcggg gatgtggatg acgcgggcgt
     1201 tcggccccaa ctgccgcacc ggggcgatat gcggatcgac ggcgcgggta aagatatcga
     1261 cctgtttgcc ccggcggccc aactcgcggg ccagctcccg cacatagacg ttcatgccgc
     1321 cggtttcctt gccccccagc gtcgccaggg gacaggtatg cacactaagc atcgctattc
     1381 gacacatgca ctcacttcct gataatcacg cgatagataa actggtcctg ggcgcccagg
     1441 tcgtacttat agcgttcgtc accccggaga aaatcgaagg tctggactcc ttcttcgatc
     1501 gcctgccgga tacgatacac cagcaaagcg aagccggcgc tataaccccc ataacgcggg
     1561 tcatacccag agttgtaaag cagtagtcgt gccgcctgcc ggaacgacag gtaggcggcc
     1621 acgggctggt cgcccgcgta gagggtggcg acctccagcc acccggtggg cgcgagggcg
     1681 gccagcagcc ggtggaagaa cgtcaccatg atcggcgtca tgaagcgggc cttggcctcg
     1741 tcgctgcggc ggtgcagcgc gataaacgtc tccagcgccg cggccagttc ggggcctttg
     1801 ttgaccgtcc gccacgtcag cggctcggga tgggattccg cgcggcgcag cttgcgccgc
     1861 agttcgtggc ggtccttctt gcccagcgcg gccaggtagt cgtcccacgt ggcgggcaag
     1921 gccagcaccg ggcacgtctc ctcgacgccg caccagacgg tgccgtgcgc ggacgccgcg
     1981 caccgggccg aggtcgcgct cgctaccggc agcgaatgga ggtcgagcac atcccagtcg
     2041 gcggcgcggc ccgcccagta ggccagcacg gcggcccagg cttcgcactc gcggcccgcc
     2101 ggggcgatga tattcaaata atcggccacg gcgatcccgc cgaccaggcg caccacccgc
     2161 tcgccgggcc ggggcgccgc ctccggcggc tcggccgttg tggctgccgc ctcgcccgcc
     2221 gctgtccggc cctcgggccg ctccgccgcg gggtcgccct gcgccgccgc gcgggccgcg
     2281 atgcgccgcc agtccgcggc gcgactcgtc tcctcggccc agccggcctc atccatggtg
     2341 gacaaataga aaggggccac ccccaccagg gtgccccctt cccgcagcaa caagagcgcg
     2401 agttgacagc tcgcactcgc gttacaacag aaagtacacc accaggcctc ctggaacggg
     2461 cggctcagga acagcgggtc ggcggccgat ccggccagca cccggtccca ctcatcgggc
     2521 gccggcccgg cggcgaactc gccgacacat tcgatctcga acgacatggt tgtgaggatc
     2581 ctccccagga tgtacgcacg attcgggcga tcggttctac cccgccgccc atactaaacg
     2641 gcggcgcgaa caggcaccgc cgccaggtag cgttttagct actatacgcc agatccgtgc
     2701 ccgcggctgt aatccagccc acgcgccggt tgctctacgg cggcttctgt ggtatcgtta
     2761 ggcggcgagg cgtaggcgcg gaggagtcga tgccgcgaat cctggtggtc gaagataacg
     2821 cgatgatccg cgagatgatg gcccggcggc tccagcggcg cggctacgag atcctggtcg
     2881 ccgccgacgg ggcggaaggc gtggcccgtg cccaggtgga ccagccggac ctcatcctca
     2941 tggacatgag tctgccggtg ctggacggct gggcggcgac ccggcgcctc aaggcggcgc
     3001 ccgccacgca ggccatcccg gtgatcgccc tgacggccca cgccatggcc ggcgaccgcg
     3061 aatcctgcct ggccgccggc tgcgacgaat acgacaccaa accggtggac tttgcccgcc
     3121 tgatcggcaa gatccaggcg ctgctggatc gcgcggcggc gtcctgatca gggccgggtc
     3181 cgcgaaactt ttgcccctgg gcggcaatga atccgtatgg cggcatcccg gcggggccgg
     3241 cctaaccccc ctgcccccct tcccggcgcg ggaaggggga atgacacggc agtaggatgg
     3301 cgacactgcc ctccccgcgg cggggagggg ttgggggaga ggttggtcgc gcgccgcagt
     3361 cgcaccaggc agcgccgctt tccgcgatcg ataacgagga gacttcggag tggtggaacc
     3421 ccgtggatct acgcggcgct tcgcggcgcg gctggatcct cgctatagct tcagcgcgcg
     3481 cctgggctta gccctcgggc tggtcgcctt cgtgacctcc atcctgatca gcctggccgc
     3541 gggctacaac agcagcgcgc aaatcaccgc cgacagcggc cagttcctgg cggaagtggc
     3601 ctttcacatg agcagccagc tcgacaccag catccaggaa cgcttccgcg atctgcaagt
     3661 gctggcgaca ctcgacacca tccgcgaccc cgccgtgccg cgcgacacca agcgcgatct
     3721 gctggaacgg ctgcaaaaca cctacaccga ttactcgtgg atggggctgg ccgacagcca
     3781 ggggcgcatc gtcgtgagca cgcgccggct gctggaaggc aacgactcgt tgcagcgcaa
     3841 atggttcgtc aacggccagc aggcgcccta tctcggccag gccgaccagg ggctgctgct
     3901 gcccaacccg gcgcccgatc ccaccgggga accgttgcgc ttcatcgacc tggccgtgcc
     3961 ggtgagcgac ctgaacgggc agccggcggg cgtgctcacc gcccacctga gttgggcctg
     4021 gggccagaag atccaggccg ggctgctgga accgttgcag acgcgccagc atgtcgaaat
     4081 gctcgtgctg acccacgagg gcaccgtctt gctcggcccg tccgagttca agagccaggc
     4141 ccagacgctg accctcgaca gcgtggccgc ggcgcgcgcc gggcacaacg cctacaagac
     4201 agaaacctgg cccgacggca agctcttcct gaccggctat gcgggtatcg ccggcgaccg
     4261 caccaacccc cgcgtgccct ggatcgtgct ggtgcggcag agcgtgaccg ccgcccaggc
     4321 cccggcgcgc gccctgcagc aggaagacct gatccgcggc atcctgctcg gcatcctgtt
     4381 tgccgtgctc agcgggctgc tggccgggcg gctggtgcgg cccctgcgcg gcatcgccgg
     4441 cgccgccgac cgcatccgcc agggcgacgc ggcggcgacg atcccggtcc accaggggca
     4501 cgacgaagtc gccgtgctgt ccgactcgct gagcggcctg gtcagcagcc tgaacgggca
     4561 gaaggccgag ctatccacgc tgaatgccca gctagagcgc gaactggccg agcgccgcca
     4621 ggctgaagac gacctgcgcc acctgcgaga cgagttggaa atccgcatcc agcggcgcac
     4681 cgccgacctg gtccacgcca accacgaact gcaaaccgag gtggctgagc gccgccgggc
     4741 cgaggctgcc ttgcagtgga gcaacgcgct gctggtggcc gagcaagagg cggccatcga
     4801 cggcatcctg gtaacggacg ccgagcggcg gacggtcagc gtgaacgggc cgttttgcgc
     4861 catgtggggg ctggcgcccg agacggcgcg caagctgggg ccggcggcgc tggtcgagtc
     4921 ggtgctgccg ctgctacagc gcccggagga gtttctggct gatctcagtg cgttgtacat
     4981 gccggagcac ctcaaggata cccgccgggc ggaaatcggc ttgaaagacg ggcggatcat
     5041 cgaccgctat gtggcgccgg tgctgtcgcc ggagggcgaa gtctggggcc gcatctggta
     5101 cttccgcgat gtcacccggc agcggcacgc cacgcaacgc ctggccgggc agcacgccgt
     5161 ggcccgcatc ctgaccgaga gtaacgcctt gtccgcggcc ctgccgcgcg tggtccaggc
     5221 cgtgctcgac accctgggtt gggacacggg cgtgatctgg atggtcgatc cgtccggcgc
     5281 ggccctgcgt agccggggcg tggtggccca cgggccggat gcgcccgccc tgcccgccgc
     5341 cgcgctggaa cagttgcagc ccgcatcggg cgccggcttg cctggccagg tctgggcctc
     5401 cggccagccc gcctgggtcg cggatctcgc cgagcagcgc gacacgccgg atgcggcgct
     5461 cctggccgat gccggcctgc gcggcgcctg tgccctaccg gtcatgcagg gcgaggcggt
     5521 gctcggcgtg ctcgaatttt gcagccgcga cccgcagatg ccgggcgccg
//
LOCUS       DASUYC010000940         3768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_666057,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000940 DASUYC010000000
VERSION     DASUYC010000940.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666057"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..873
                     /locus_tag="VF276_15370"
     CDS             70..873
                     /locus_tag="VF276_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX6608279.1"
                     /translation="MPPTLIDSHAHVDAPEFAADRDAVIARAHAAGVTRMITVGYDQA
                     TWDTATALVQAHPGAGLYLALGIHPNSAYETSDATLAALAARIGAGRTGTGPRVVGLG
                     ETGLDYYREWATPEQQRASFRAQLALARELDLPVIIHDRDAHADIMDILRRDGQGTRG
                     VMHAFSGDVAMAEECLRRGYLISLAGPVTFPKAADRHAVARAVPLAGLLVETDCPYLT
                     PVPFRGKRNEPAYVAHTAARIAELRGVPLDTVAAATTANAVALFGLDSC"
     gene            867..1916
                     /locus_tag="VF276_15375"
     CDS             867..1916
                     /locus_tag="VF276_15375"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX6608280.1"
                     /translation="MLNLYQLQMFLAVVDSGSFSAAAEHLHVTQPAVSEGVRALEQRL
                     NMRLFDRRGHRAALTPEGANLIATARHLLALADHAEQGLLGQKGVLSGALRLATGTSA
                     GAPLLGERLGAFAGTHPAVRLTLVARAPAAVLDGLREGEFDTGFVGAPLPAPRLHYLP
                     LAADEWLLLAPPGHAWAGPPAPKGAANEAPAAADPPPTEAAPVKRKRGRPPARRPDPV
                     PPPLPTVALAALRDQPLVLESADAPLGAQARRELRDLLEERDVPWAGLRPVLELGSPL
                     QVLRAVEAGAGVALLPAAELAGYPGPAQRVRPEGRPLTRLLFAVRDSRTPLSPVLAAW
                     WDFLTAHSAGPDPAG"
     gene            1959..>3768
                     /gene="ppsA"
                     /locus_tag="VF276_15380"
     CDS             1959..>3768
                     /gene="ppsA"
                     /locus_tag="VF276_15380"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015174316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEX6608281.1"
                     /translation="MRSYVLGFQDIDRTQLNLVGGKGANLGELCKIAGIRVPDGFCLS
                     TAAYQRIISEAPAIHPLLDRLAPLKANDRDRIAELSGEIRRLIAGVAVPQDIRAEIAR
                     HLTRLGEHSACAVRSSATAEDLPGASFAGQQDTYLNIIGEQAILEHIRQCWASLFTAR
                     AVTYRLQNGFDHRKVSLAVVVQQMVFAQAAGIMFTADPVTGNRKVLSIDAGFGLGEAL
                     VSGVVNADTYKVRNGTVLDKTVSTKKRAVYALPGGGTQVQEVAPEQQNRPVLSDEQIV
                     QLERIGRVIEDHFGFPQDIEWCLTDDGFYIVQSRPITTLFPIPEANDQENHVYISVGH
                     QQMMTDPIKPLGLSFFLLTTPAPMRPAGGRLFVDVTRLLASPAGRASLLAGMGRHDPL
                     LKDALETLIERGDFIKPLPTDETGAGPARSPRTVSPASFDPQTEYDPRIVADLIAHSQ
                     ASIAELKRQIATKSGSDLLDFILEDIQQLRTLLFDPRSSEVFMTAMNASAWLNENIQE
                     WLGERNVAGTLSQSVPNNITSEMGLELLDLADVIRPYPEVVEYLRHASEDRFLDGLLQ
                     VRGGKETRAALDAYLGKYGMRGAGEIDITRTRWSENP"
BASE COUNT          597 a         1395 c         1222 g          554 t
ORIGIN      
        1 ggctgacggc tgacggctga cggctcgccc gccttgacgc cccgcgcggc cctgtgctag
       61 actcggccca tgccacctac gttgatcgat tcccatgccc atgtggacgc gcccgagttc
      121 gccgccgacc gggacgccgt gatcgcccgc gcccacgccg ccggggtcac gcgcatgatc
      181 accgtcggct acgaccaggc gacgtgggac acggccaccg cgctggtcca ggcccacccc
      241 ggcgcgggcc tctacctggc gctcggcatc caccccaaca gcgcgtatga gaccagcgac
      301 gccacgctgg ccgcgctggc cgcgcgcatc ggggccgggc gcaccggcac cgggccgcgg
      361 gtggtcggcc tgggcgagac ggggctggac tactaccgcg agtgggccac gccggagcag
      421 cagcgggcca gtttccgcgc ccagttggcc ctggcccgcg agctcgacct gccggtgatc
      481 atccacgacc gcgacgccca cgccgatatc atggacatct tgcgccgcga cggccagggc
      541 acccgcggcg tcatgcacgc cttcagcggc gatgtagcca tggccgagga gtgcctgcgg
      601 cggggctacc tgatctcgct ggccgggccg gtcacgttcc ccaaggccgc cgaccgccac
      661 gccgtggcgc gcgccgtgcc gctcgcgggc ctgctggtcg agaccgattg cccctacctg
      721 acgcccgtcc cgttccgcgg caagcgcaac gagccggcct atgtggccca cacggcggcc
      781 cgcatcgccg agttgcgggg ggtgccgctg gacaccgtcg ccgccgccac cacggccaac
      841 gccgtggccc tctttggact ggactcgtgc tgaacctcta ccagttgcag atgttcctgg
      901 ccgtggtcga tagcgggtcg ttctcggcag ccgccgagca cctgcacgtc acccagcccg
      961 ccgtcagcga aggggtgcgc gcgctggagc agcggctgaa catgcggctg ttcgaccgcc
     1021 gcggccaccg cgccgcgctc acgccggagg gcgccaacct catcgcgacc gcccgccacc
     1081 tgctggcgct ggccgaccac gccgagcagg ggctgctggg ccagaagggc gtgctcagcg
     1141 gggcgctgcg gctggcgacc ggcacctcgg cgggcgcgcc gctgctgggc gagcgcctgg
     1201 gcgcctttgc cggcacccac cccgccgtgc gcctgaccct ggtggcccgc gcccccgccg
     1261 ccgtgctcga cggcctgcgc gagggcgagt tcgacaccgg cttcgtcggc gccccgctgc
     1321 ccgcgccccg gctgcactac ctgcccctcg ccgccgacga gtggctgctg ctcgcgccgc
     1381 ccggccacgc ctgggccggc ccgccggccc ctaaaggggc agcgaatgag gcccccgccg
     1441 ccgccgaccc gccgcccacc gaggccgcgc cggtcaagcg caagcgcggt cgcccgcccg
     1501 cccgccgccc cgacccggtc ccgccgccgc tgcccaccgt ggccctggcc gccctgcgcg
     1561 accagcccct ggtgctcgaa agcgccgatg cgcccctggg cgcccaggcc cggcgcgaac
     1621 tgcgcgacct gctcgaagag cgcgacgtgc cctgggccgg cctgcgcccc gtgctcgaac
     1681 tgggcagccc gctccaggtg ctgcgcgccg tggaggccgg cgccggggtg gccctgctgc
     1741 ccgccgccga gctcgccggc taccccggcc cggcccagcg cgtccgcccc gaaggccgcc
     1801 cgctgacccg cctgctcttc gccgtccgcg acagccgcac cccgctctcg cccgtgctcg
     1861 ccgcctggtg ggacttcctc accgcccaca gcgccggccc cgacccggcg ggctagcgta
     1921 tggcgtatgc ggaagaacca acagtgagag gtaaagccat gcgttcatat gtgctgggtt
     1981 tccaggacat cgacagaacc caactcaacc tcgtcggggg taaaggcgcg aacctggggg
     2041 aactctgcaa gatcgccggc attcgcgtac ccgacggctt ttgtctttcg accgccgcct
     2101 accagcgaat catcagcgaa gcgcccgcaa tccacccgtt gcttgatcgc ctggcgccgc
     2161 taaaagcaaa tgaccgggat agaatcgctg aactgagcgg cgagattcgc cggctcatcg
     2221 caggggtagc cgtcccgcaa gacattcggg cagagatcgc ccgccacctg acccggcttg
     2281 gcgaacacag cgcctgcgcc gtacgctcca gcgccaccgc cgaggacttg ccgggggcgt
     2341 ccttcgcggg ccagcaggat acctatctga acattatcgg ggagcaggcc atcctggagc
     2401 atattcgcca gtgctgggcc tcgctcttta ccgcgcgggc cgtaacctac cgcctgcaaa
     2461 atggcttcga ccaccgcaaa gtctctctgg cggtggttgt tcagcagatg gtcttcgcgc
     2521 aggcggcggg catcatgttt acggccgatc cggtcaccgg caacaggaag gtgctgtcca
     2581 ttgatgcggg cttcggcctc ggcgaggccc tcgtctccgg cgtggtgaac gccgatacct
     2641 ataaggtgcg taacggcacg gtcctcgaca agacggtatc cacaaagaag cgggctgttt
     2701 atgccctgcc gggagggggc acgcaagtac aggaagtcgc gccggagcag cagaacaggc
     2761 cggtgctgtc agacgagcag attgtgcaac tggagcgcat aggccgcgtg atcgaagacc
     2821 atttcggttt cccccaggac atcgaatggt gtctgaccga tgatgggttt tatatcgtcc
     2881 agagccggcc catcacgacg ttgttcccca tcccggaagc gaacgaccag gaaaaccatg
     2941 tgtacatctc cgtcggccac cagcagatga tgaccgaccc catcaaaccg ctgggactct
     3001 ctttcttcct gctcacgacc ccggcgccga tgcgcccggc gggcggccgg ttgtttgtcg
     3061 acgtgacccg gctcctcgcg tccccggccg gcagagcaag cctgctggcc ggcatgggcc
     3121 ggcacgaccc gctcctcaag gacgcgctgg agaccctcat cgagcggggg gacttcatca
     3181 agccgttacc caccgatgaa acaggcgcgg gtcccgccag gagccccaga accgtgtcgc
     3241 ccgcgagttt cgatccccag accgaatacg acccccgcat cgttgccgac ttgatcgcgc
     3301 atagccaggc ctcgatagcc gagttaaagc ggcaaatcgc gacgaaatcg ggcagcgatt
     3361 tgctcgattt cattctcgaa gatatccagc aattgcggac actcctgttc gatccccgca
     3421 gctcggaggt cttcatgacg gccatgaatg cgtccgcgtg gctcaacgag aacatccagg
     3481 agtggttggg ggaaagaaac gtggccggca cgctctcgca atccgtaccc aacaatatca
     3541 cgtcggagat gggcctggag ctgctagacc tggcggatgt tatccgcccg tacccggaag
     3601 tggtcgagta tctgcgccac gcaagcgagg atcgcttcct ggacggcctg ctgcaggtgc
     3661 gcggtggcaa ggagacccgc gccgccctcg atgcgtatct cggcaaatac gggatgcgcg
     3721 gcgccggcga aattgatatc acccgcaccc ggtggagcga aaacccgg
//
LOCUS       DASUYC010000941         2274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_257118,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000941 DASUYC010000000
VERSION     DASUYC010000941.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257118"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VF276_15385"
     CDS             <1..521
                     /locus_tag="VF276_15385"
                     /inference="COORDINATES: protein motif:HMM:NF012481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEX6608282.1"
                     /translation="VAGRPATRTGRQQRGSKMNTLVIYDSKFGNTEKVAQAIAEVLQA
                     HGLVRLVPVAEAETVTLDGVDLLAAGGPTQGHGISPAMTAWLTNLPRAAAQGLPTVTF
                     DTRFRKPAWLTGSAAHSLAKRLEQHGCRMLVPPESFFVAGTEGPLEDGERDRAAAWAR
                     DIAARLPVAAPA"
     gene            668..2119
                     /locus_tag="VF276_15390"
     CDS             668..2119
                     /locus_tag="VF276_15390"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HEX6608283.1"
                     /translation="MLAHKRVVQRLPAVALILMLLPLSGGAAGAAPGVHARFQQYYEQ
                     YQGLRVLGHPLTDLTQAGGYPAQYFEKGCIEDHQGAGADPHWAFMYSRLTAILMERDP
                     AGPVSATSVTYGDLQRAAAPALRRPAPAGFTGGTRGVHDGQFVPFDANLRPAPGHIVA
                     PYFWAYITRADLFPGGWLHDIGLPMTEPLAASVVKEGAARPIILQAFERTVLSYDPQN
                     PAGWQVERANIGADSLRTLPRPVGEATISIPAPGARVLLPLHILAQTGRPGDQVTAVL
                     TWRDGTTLTNRLPVLPGPAGQGLVVANLDWVNLLQPPEPPTQPATLALRDAAGRLLAS
                     QEVTLVSPNDPGTQQIQLYWTVSGAEVVTPQLRRILKTARIGTAALEELLWGPPAVSQ
                     VGYHTALPAPEQVLGYPGRAADWGPRVTLRSLNISGGVATADFSQELRAYGGGSLRVR
                     LIRAQITRTLMQFPTVQQVRIAIEGRTDGVLEP"
BASE COUNT          336 a          874 c          740 g          324 t
ORIGIN      
        1 gcgtcgccgg gcgcccggcg acccgcacgg gtagacaaca gcgaggcagt aagatgaaca
       61 cactcgtcat ttacgactcg aaatttggca acaccgagaa ggtcgcgcag gcgatcgccg
      121 aggtgttgca ggcccacggc ctggtccggt tggtgcccgt cgccgaggcc gagacggtca
      181 cgctggacgg cgtggatctg ctcgccgccg gtggcccgac ccagggccat gggatcagcc
      241 ccgcgatgac cgcctggctc accaacctgc cgcgcgccgc cgcgcagggt ctccccaccg
      301 tcaccttcga cacccgcttc cgcaagccgg cctggctcac ggggtctgcc gcgcacagcc
      361 tggcaaagcg cctggaacag cacggctgcc ggatgctcgt accgccggag agcttcttcg
      421 tcgccggcac tgaggggccg ctggaggatg gcgaacggga tcgcgccgcc gcctgggccc
      481 gcgacatcgc cgcgcggctg cccgtcgccg cccccgccta gctgccgggc agccgttttg
      541 cgtccccgta gccggccacg gtaattgcgc ggcgtagagg ctggttgccc accggcccct
      601 gcgcccatga gaatccccgt gggaaaccac tcacttccgg ctgcgctgaa ctggaaaggt
      661 cataccgatg cttgcccaca agcgggtcgt gcaacggctg ccggccgtcg cgctgatcct
      721 tatgctgctg ccgcttagcg gcggcgcggc cggcgccgcg cccggcgtgc atgcgcggtt
      781 ccagcaatac tatgagcagt accaggggct gcgcgtgctc ggccatcccc tgaccgatct
      841 cacgcaagcc ggcggctacc cggcccagta tttcgagaag ggttgtatcg aagatcacca
      901 gggcgcaggg gccgatccgc actgggcttt catgtatagc cggctcaccg ccatcctgat
      961 ggagcgcgat cccgccggac cggtcagcgc caccagcgtc acctacgggg atctccaacg
     1021 cgctgccgcg cccgcactgc gccggcccgc gccggccggc tttaccggcg ggacccgagg
     1081 ggtgcatgac ggccaattcg tgcctttcga cgccaatctc cgccccgccc ccggccacat
     1141 cgtggcccca tatttctggg cctacatcac ccgcgccgac ctcttccccg gcggctggct
     1201 gcacgatatc ggcctgccca tgaccgagcc gctggccgcc agtgtggtca aggagggcgc
     1261 tgcccggccc atcatcctgc aagccttcga gcgcaccgtc ctgagctacg acccgcagaa
     1321 ccccgccggg tggcaggtgg agcgcgccaa tatcggcgcc gattccctgc gcaccttgcc
     1381 ccgtccggtg ggggaggcca cgatcagcat cccggcccct ggcgcgcggg tgctgctgcc
     1441 gctgcacatc ctggcgcaga ccgggcgccc cggcgatcaa gtcaccgccg tgctcacctg
     1501 gcgcgacggc accactctca ccaaccggct gccggtgctg ccgggtcccg ccgggcaggg
     1561 gctggtcgtc gccaacctcg actgggtcaa cctgttgcaa ccgcccgaac cacccacaca
     1621 acccgccacc ctggcgctcc gcgacgccgc cgggcgcctg ttggcgtctc aagaggtgac
     1681 gctggtcagc ccgaatgacc ccggcacgca gcagatccag ctctactgga ccgtcagcgg
     1741 cgccgaggtc gtcaccccgc agttgcgccg gatcctgaaa acggcgcgga tcggcactgc
     1801 cgcgctggaa gaactgctgt gggggccgcc cgccgtcagc caggtcggct accacaccgc
     1861 gctgccggcg cccgagcagg tgctcggcta tccgggccga gccgccgatt gggggccgcg
     1921 cgtcaccctg cgtagcctga acatcagcgg cggcgtggcc accgccgact tctcccagga
     1981 actgcgcgcc tacggcggcg gatcgctgcg cgtgcgcctg attcgcgcgc agatcacgcg
     2041 cacgctcatg cagttcccga ccgtccaaca ggtgcgcatc gctatcgagg gccgcacgga
     2101 cggcgtcctc gaaccctaag ccgactcgct cccgcgccac atgcctctct cccccttccc
     2161 gctctgggaa gggggccggg gggtcaggcc cgcgggcgcc cgctgtggat ggcccaatcg
     2221 accaggcccg acccgcccgc ctgcggcatg agccggaggg cccgcggcag gacc
//
LOCUS       DASUYC010000942         3223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_974586,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000942 DASUYC010000000
VERSION     DASUYC010000942.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974586"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VF276_15395"
     CDS             <1..417
                     /locus_tag="VF276_15395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608284.1"
                     /translation="RDIPDNTRVTYPVSFSSESHLFFLFALTESADRMPVGSWLAPSQ
                     IVRLDDRFIEKDIREFFITLLRYHMLAYDYATPQDRYQGVVVDSTGMLSRALAQHRIN
                     LRGLIRSAVTVCDLLYLYADYQPDMLLDELKRGLKV"
     gene            414..2483
                     /locus_tag="VF276_15400"
     CDS             414..2483
                     /locus_tag="VF276_15400"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX6608285.1"
                     /translation="MNPQALKAALPRTWGAFFGRHGNFTAAQLAAIPAVLAGENVLLA
                     APTASGKTEAALAPLIERHLPPLRPPHSLAILYLLPTRALINDVAARLAAPLATLRIS
                     LAQKTHDLNTFDPQRPADLLLTTPESLDALFAGQAKALAGVRAVVIDELHIFAGTARG
                     DQLQVLLNRLRQVRAYAGTTGDAEDAAVQYVALSATLGDPVETAARYFAGAQVVEVGG
                     ARAVQAEQIALDKDSPGALLAYVQTFRRRGWRKALAFCNTRAEIESYAAAVRAAGSPV
                     GDAVYVHYSNLEWDRRAEIERQFAQADAALCFASSTLELGIDIGSIDVALLIGAPGSA
                     AAFAQRVGRANRRRQTVQVACFYRTPLERVLFDALLAGQDYEAAPGAFRPAVAIQQIF
                     SLLKQSPTGAVRLAPLLELFGDRLTEADLQAILGTLQALDYLQSGRPGEWRAGARLHR
                     LVDQQATEHAPLSLHSNLAVADTPPIKIRDQYSQRVVASVDRQWFDRDLLTLEGRPLN
                     VEWADGESLWVSAYRGADTAEHLRYLSSRQVLSYDLARRLPAQVGLAPRAAPLVPYND
                     GWLWFHWLGDVYGRAALDLLRYTLAASESPQPGICLALRDELRALPVWTADQVTRYLH
                     DEYRRYEPLLALGAYHHLLPTELRRRAVVAQFDVPRFLRAVGALQIMRAAEAVNQAMQ
                     DLVIESR"
     gene            complement(2767..>3223)
                     /locus_tag="VF276_15405"
     CDS             complement(2767..>3223)
                     /locus_tag="VF276_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015622785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX6608286.1"
                     /translation="VQKPHPPLWVGGGGEKVTLKLVAKYADACNITARDPAMCRHKFD
                     VLREHCAKLDRDYNSIIKSNHIFMYLLEPGQSPDDAVRDARRLAKSDEPTLESLHKTN
                     IIGTAEEAIPVLQQLVEAGTEYFVIYFRNNLANLKSYQIFAEKIMPAFR"
BASE COUNT          481 a         1058 c         1120 g          564 t
ORIGIN      
        1 cgggacattc ccgacaacac ccgcgtgacc tacccggtca gcttctcgtc cgagtcgcac
       61 ctgttctttc tgtttgcgct aaccgagagc gcggatcgga tgccggtggg cagttggctg
      121 gccccgtcgc agatcgtgcg cctggatgac cgcttcatcg aaaaggacat ccgcgagttt
      181 ttcatcacgc tgctgcgcta tcatatgctg gcgtatgatt acgccacgcc ccaggaccgc
      241 tatcagggcg tggttgtcga ctcgaccggg atgctgtcgc gggcgctggc gcagcaccgc
      301 atcaatctgc gcggcctgat ccgtagcgcg gtcacagtct gcgatctcct gtacctgtac
      361 gccgattacc agccggacat gctgctggac gaattgaagc gggggctgaa ggtgtgaacc
      421 cgcaggcgct caaggcggcg ctgccccgca cctggggcgc gttctttggc cggcacggca
      481 atttcacggc ggcacaactc gccgcgatcc ccgccgtgct ggccggggaa aacgtgctgc
      541 tggcggcgcc cacggccagc ggcaagaccg aggccgcgct cgccccgctc atcgagcgcc
      601 atctgccgcc gctgcgcccg ccgcacagcc tggcgattct ctatctgctg cccacgcgcg
      661 ccttgatcaa cgatgtggcg gcgcgtctgg ccgcccccct cgcaacgctc cgcatctccc
      721 tcgcgcagaa aacgcacgac ctgaacacct tcgatcccca gcgcccggcc gatctgctgt
      781 tgaccacgcc ggagtcgctg gacgcgctgt tcgccgggca ggcgaaggcc ctggccggcg
      841 tgcgcgccgt ggtgatcgac gagttgcaca ttttcgccgg cacggcgcgc ggcgaccaac
      901 tgcaagtgct cttgaaccgc ttgcgccagg tgcgcgccta tgccggcact accggcgacg
      961 ccgaggatgc cgcggtgcag tacgtggcgc tgtcggccac gctaggggac ccggtggaga
     1021 ccgccgcgcg ctactttgcc ggggcgcaag tagtagaggt ggggggcgcc cgcgccgtgc
     1081 aggccgagca gatcgcgctc gataaggatt cgccgggcgc cttgctcgcc tacgtgcaga
     1141 cgttccggcg gcgcggctgg cgcaaggcgc tggccttctg caacacacgc gccgaaatcg
     1201 aaagctatgc cgccgctgtg cgtgcggcgg gttcgcccgt cggcgacgcc gtgtatgtcc
     1261 attactcgaa tctcgaatgg gaccggcgag ccgagatcga gcggcaattc gcgcaggcgg
     1321 acgccgcgct ctgcttcgcc agcagcacat tggaactggg gatcgacatc ggcagcatcg
     1381 atgtggcgct gctcatcggg gcgccgggca gcgcggcggc gttcgctcag cgcgtggggc
     1441 gggccaaccg gcgccggcag accgtgcagg tggcgtgctt ctaccgcacg ccgctggagc
     1501 gagtcctgtt cgacgcactg ctggccgggc aggactacga ggcggcgccc ggtgcctttc
     1561 gcccggcggt tgccatccag cagattttca gcctgctcaa gcagagtccg accggtgcgg
     1621 tgcgcctggc gcccttgctc gaactattcg gcgaccggct cacggaggcg gatctccagg
     1681 cgattctggg cacgttgcag gcgctggact acctgcaaag cgggcggccc ggcgaatggc
     1741 gggcgggggc gcgcctgcac cgcctggtcg atcagcaggc cacggaacac gccccgctga
     1801 gcctgcacag caacctcgcc gtggccgaca cgccgccgat caagatccgt gaccagtaca
     1861 gccagcgggt cgtggcgagc gtggaccgcc agtggttcga ccgcgacctg ctcacgctgg
     1921 agggccgccc gctcaacgtg gagtgggccg acggcgagtc gctgtgggtg tcggcctacc
     1981 ggggcgccga taccgcggag cacctgcgct atctgtcgag ccggcaggtg ctgagctacg
     2041 atctggcgcg ccggctgccg gcgcaggtgg gattggcacc ccgcgcggca ccgctggtgc
     2101 cctacaacga cgggtggctg tggttccact ggctgggcga tgtgtacggg cgggcggcgc
     2161 tcgatctcct gcgctacacg ctggccgcga gcgagtcgcc acagccgggc atctgcctgg
     2221 cgctgcgcga cgaactgcgc gcgctgccgg tctggacggc cgatcaggtc acgcgctatt
     2281 tgcacgacga gtaccggcgc tacgagccgc tgctggcgct gggcgcctac caccacctgc
     2341 tgcccacgga gctgcgccgc cgcgcggtcg tggcgcagtt cgacgtgccg cgcttcctgc
     2401 gcgccgtggg cgcgctccag atcatgcggg cggcggaagc ggtgaaccag gcgatgcagg
     2461 atctggtgat agagagtcgt tagcgcaggg tacgagggca ccgcgcccgc cgcgtgcggc
     2521 atcccggctt gcgccccacg agacgagact cttcgctccg ctcagagtga caaggttatg
     2581 ggggccgaga ctctgccctg tgctacgcta acaagggcag ggcgcgccgg gcggcaaggc
     2641 gggtttgaaa cccgccccta caccgataac ccggcttttt acagttcggc gatttcgttt
     2701 aggcaagatg aaggccggcc acgcacctat acgtggtcgg ccttcgtctt gctcggtggc
     2761 aggtgtttat cggaaagcgg gcatgatctt ttcggcaaag atctggtagc ttttcaggtt
     2821 ggcgaggttg ttgcggaagt agatgacgaa gtattcggtg cccgcttcga ccagttgctg
     2881 gagcaccggg atggcttcct cggcggtgcc gatgatgttg gttttgtgca ggctttccag
     2941 cgtcggctcg tcgctcttgg cgaggcggcg ggcgtcgcgc acggcgtcgt cgggactctg
     3001 gccgggttcc agcaggtaca tgaagatgtg gttgctcttg atgatgctgt tataatcccg
     3061 atccaatttc gcgcagtgct cgcgcagcac gtcgaacttg tggcggcaca tcgccgggtc
     3121 gcgggcggtg atgttgcagg cgtcggcgta tttggcgacg agcttaagcg tgaccttctc
     3181 accgccgccg ccgacccaca gcggagggtg gggcttttgc acg
//
LOCUS       DASUYC010000943         2296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1373858, whole genome shotgun sequence.
ACCESSION   DASUYC010000943 DASUYC010000000
VERSION     DASUYC010000943.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1373858"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VF276_15410"
     CDS             complement(<1..353)
                     /locus_tag="VF276_15410"
                     /inference="COORDINATES: protein motif:HMM:NF014085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608287.1"
                     /translation="MIDAIPGFDAGRVGPAPGGGRLFAVVPVKSLETAKSRLRPTLSG
                     RQRAGLAARLLGGVLRALNDSDVADAVAVVSPDPRVLRLAAGVGAVGLRQQTGDLNAA
                     LDLARAWAVAGGADT"
     gene            complement(350..1309)
                     /gene="cofD"
                     /locus_tag="VF276_15415"
     CDS             complement(350..1309)
                     /gene="cofD"
                     /locus_tag="VF276_15415"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HEX6608288.1"
                     /translation="MIQVVALAGGVGGAKLAQGLYAALPPASLLVLVNTADDFTLHGL
                     RICPDLDTVLYTLAGLANPQTGWGIAGDTFATLDMLTRYGAEDTWFQIGDRDFATHIR
                     RTQRLAAGAPLSAVEADLARVLDVRATLLPMADAPVATLVDTDAGRLAFQDYFVRRRH
                     SDNVRGIVFDGIAEAAPPESLAPALATAEAIVFCPSNPLVSIGPILAVPGLRDLLVGA
                     PAPRVAVSPIVGGKALKGPADQMLATLGHEVSAYGVAALYQGLIDGMVIDEADAALAP
                     RIADLGMRVQVAPTIMRDEADRKALAETVLAFCADLAGERGRQ"
     gene            complement(1306..1725)
                     /locus_tag="VF276_15420"
     CDS             complement(1306..1725)
                     /locus_tag="VF276_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX6608289.1"
                     /translation="MALTHELNDAARAFLQEPRFAVVATVSPNGTPQQTVNWYLVEGD
                     EIMFNTRRGRIKDRNLSRNQHVSICIEDNYRYVTIRGQVQIIEDQATAQADIKRLAIR
                     YHGREKGENMAATGFTKEERISYRVKLDHVSVYGFDS"
     gene            complement(1822..>2296)
                     /locus_tag="VF276_15425"
     CDS             complement(1822..>2296)
                     /locus_tag="VF276_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011604580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6608290.1"
                     /translation="MWSGEDSPYNGKHYHLDRTLNVPQALSKPHPPILIGGTGEQKTL
                     RFVAKYAEACNLFMRMGDDVVRHKLEVLRGHCADLNRPYSEIDKTSLDSLEIASSNSK
                     EGMSGQQLVDYVGQAAELGFDQAIFSIRNVSDPGVFDTIAKVESQIAAVPEGGRK"
BASE COUNT          343 a          797 c          780 g          376 t
ORIGIN      
        1 aacgtatcgg cgccgcccgc cacggcccag gcccgcgcca ggtccagcgc cgcattcagg
       61 tcgcccgtct gctggcgcag gcccacggcc ccgacgccgg ccgccaggcg cagcacccgc
      121 gggtcggggc tgaccacggc cacggcgtcc gccacgtccg agtcgttcag ggcgcggagc
      181 accccaccga gcaggcgcgc cgccaacccc gcgcgctggc gccccgagag ggtgggccgc
      241 agccggcttt tggccgtctc cagcgacttg accggcacaa cggcgaagag ccgcccccca
      301 cccggcgccg gaccgacccg ccccgcatcg aagccgggta tcgcgtcgat cattggcggc
      361 ctcgctcgcc tgcgaggtcc gcgcagaacg ccagcaccgt ctcggccagg gctttgcggt
      421 cggcctcgtc gcgcatgatc gtcggcgcga cctgcacgcg catgccgaga tcggcgatgc
      481 gcggcgccag ggccgcgtcg gcctcgtcga tgaccatgcc gtcgatcagc ccctggtaga
      541 gcgccgccac cccgtaggcc gacacctcgt ggcccagcgt cgccagcatc tggtcggcgg
      601 ggcctttcag cgccttgccg ccgacaatcg ggctgaccgc cacgcgcggc gcaggtgccc
      661 cgaccagcag gtcgcgcagc cccggcacgg ccaggatcgg cccgatgctg accagcgggt
      721 tgctggggca gaagacaatc gcttccgccg tggcaagcgc gggcgccagc gactccggcg
      781 gcgcagcttc cgcgatgccg tcgaagacga tgccccgcac attgtcgctg tgccggcggc
      841 ggacgaaata gtcctggaaa gccagccgcc cggcatcagt gtccaccagc gtggcgaccg
      901 gcgcgtcggc catcggcaag agcgtggcac gaacgtccag gacgcgcgcc agatccgcct
      961 ccaccgcgct cagcggcgcg cccgccgcca gccgttgtgt gcgccggatg tgtgtggcga
     1021 agtcccggtc cccgatctgg aaccaggtgt cctccgcgcc gtagcgggtg agcatgtcca
     1081 gcgtggcgaa cgtgtccccg gcgatgcccc agccggtttg cgggttcgcc agcccagcca
     1141 gggtatagag cacggtatcg aggtcggggc agatgcgcag gccgtgcagc gtgaagtcat
     1201 ccgccgtgtt gaccagcacc agcaggctgg cgggcggcaa cgccgcgtac aggccctggg
     1261 ccagcttggc gccgcccact ccgcccgcga gcgcaacaac ctgaatcatg agtcgaaacc
     1321 gtagacggag acgtggtcca acttaacgcg atagctgatc cgttcctcct tggtgaagcc
     1381 ggtcgcggcc atgttctcgc ccttctcgcg gccatggtag cggatcgcca gccgtttgat
     1441 gtcggcctgc gccgtggcct ggtcttcgat gatctgcacc tggccgcgga tggtgacgta
     1501 gcggtagtta tcctcgatac agatcgatac gtgctggttg cgcgacaggt tgcggtcttt
     1561 gatgcggccc cgccgggtgt tgaacatgat ctcgtcgccc tccaccaggt accagttgac
     1621 ggtttgctgc ggcgtgccgt tcggcgaaac ggtggcgacg acggcgaagc gcggctcctg
     1681 caagaaggcg cgagccgcgt cgttgagttc gtgcgtgaga gccatacggg acgagtctcc
     1741 tcgatctagg ttcgggatgg tgaggacccg gcgtaccggg tcctcacggg tatcagcgcg
     1801 accgggcgca caccgttgcg gctacttgcg tccgccctcc ggcaccgccg cgatctgcga
     1861 ctctactttg gcgatggtat cgaacacgcc gggatcgctc acgttgcgga tgctgaagat
     1921 cgcctgatca aagccgagtt cggcggcctg gcccacgtaa tcgacgagtt gctggccgct
     1981 catgccctcc ttgctgttgc tgctcgcgat ttcgaggctg tcgagactgg tcttgtcgat
     2041 ctcgctgtaa ggccggttca ggtcggcgca atggccgcgc agcacctcca gcttgtggcg
     2101 caccacgtcg tcgcccatgc gcatgaagag attgcaggcc tcggcgtact tggcgacgaa
     2161 gcgcaacgtc ttctgctcgc cggtgccgcc gatgaggatc ggtgggtgag gcttgctgag
     2221 cgcctgcggc acgttgagcg tgcggtcgag atgatagtgt ttgccgttat aggggctgtc
     2281 ctcgcccgac cacatt
//
LOCUS       DASUYC010000944         2471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1082530, whole genome shotgun sequence.
ACCESSION   DASUYC010000944 DASUYC010000000
VERSION     DASUYC010000944.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082530"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VF276_15430"
     CDS             <1..216
                     /locus_tag="VF276_15430"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEX6608291.1"
                     /translation="LLRKEESSKGVEVTITSDRVVIDLYVILEYGTRIATVAENIMSS
                     VKFAVEQALGTPAVEVNVNVQGIRVSE"
     gene            241..1941
                     /locus_tag="VF276_15435"
     CDS             241..1941
                     /locus_tag="VF276_15435"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEX6608292.1"
                     /translation="MADLKSNLDVSSAPGTGGTWDGVQWKQAVAAASRWLDRHAAGIN
                     ALNVFPVPDGDTGTNMTLTLQAAAKQVAGSVSHSVAEIASGMSYGALMGARGNSGVIL
                     SQVLRGFHRGLGNSDTLGAAEWAAAMRDAANTAYQAVLKPVEGTMLTVIREMADGAEA
                     AAGTGDLLAVMSATVEAGRASVERTPTLLQKLRDAGVVDAGGQGLYVIFEGLLRFARG
                     ESVEGGAIETASGAPLELAPDGVHVAHGEYGYCTNFILVGKDLGFPEVREWIAQHGDS
                     AVVVGDDRLIKVHVHTEQPGEILTYVCARGNLRQVAITDMQEQHEEFLDMHEQAAAKA
                     PAAPAVPATGDPALSKIATVAVVSGAGLAEIFRSMGATALVEGGQTMNPSTEELLRTI
                     EALPHDEVIILPNNGNIVMAAKQTQALSRKRVAVVPTDTIPQGMAALVAFNYEADLDT
                     NARAMDAAAQNVETGEITTAVRQVSMNGIEIQIGDVIGLHNGTLVIAGHSNEEVAHDL
                     LAQLNAGDRELITIYYGQDVTAEQAETFADQIRAQYPDQEVEALYGGQPFYAYIISVE
                     "
     gene            1981..>2471
                     /locus_tag="VF276_15440"
     CDS             1981..>2471
                     /locus_tag="VF276_15440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEX6608293.1"
                     /translation="MPTIRVVTDSTCDLPDSLVREYGITVIPVSVQIGSHTYLDRVNL
                     TTDELLRHLQAGDPRPTTAPPAVAAFEQVYRQLQANRPCDGIVSIHVSSRLSGTYNAA
                     LMARDAFLNSSFPVGVMDSQAASMGLGLVVLAAARKAAAGGTLSEVMNAAQRMIHQVH
                     IAF"
BASE COUNT          446 a          840 c          821 g          364 t
ORIGIN      
        1 ctgctgcgca aagaagagag cagcaaaggc gtggaagtca cgatcaccag cgaccgcgtg
       61 gtgatcgatc tgtatgtgat cctcgaatac gggacgcgca tcgccaccgt ggccgagaac
      121 atcatgagca gcgtgaagtt cgccgtggag caagccctgg gcaccccggc ggtcgaagtc
      181 aacgtcaatg tccagggtat tcgcgtcagc gagtaataca cggcagagag aggacccagc
      241 gtggccgacc tgaaaagcaa tctcgatgtg agcagcgcgc cgggcaccgg cggcacgtgg
      301 gacggggtgc agtggaagca ggccgtggcg gccgccagcc gctggctgga tcgccacgcg
      361 gcgggcatca acgcgctcaa cgtctttcca gtccccgacg gcgacacggg tacgaacatg
      421 acgctcaccc tgcaagccgc cgccaagcag gtggccgggt cggtcagcca ctcggtggcc
      481 gagatcgcgt cgggcatgtc ctacggcgcg ttaatgggtg cgcggggcaa ctcgggcgtg
      541 atcttgtcgc aggtcttgcg cggcttccac cggggcctcg gcaacagcga cacgctgggc
      601 gcggcggaat gggccgccgc catgcgcgat gccgccaaca ccgcctacca ggccgtgctc
      661 aagcctgtcg agggcaccat gctgacggtc atccgcgaga tggccgacgg cgccgaggct
      721 gccgcgggca cgggcgactt gctggccgtg atgagcgcca ccgtcgaggc cgggcgggcc
      781 tcggtcgagc gcactcctac gctgctgcaa aagctgcgcg acgcgggcgt ggtcgacgcc
      841 gggggccagg gcctctacgt catcttcgag ggcctgttgc gctttgcccg cggcgagtcg
      901 gtggagggcg gcgccatcga gacggcgagc ggcgcgccgc tggaactcgc gcccgacggc
      961 gtgcatgtcg cgcacggcga gtacggctac tgcaccaact ttatcctggt cggcaaggac
     1021 ctggggttcc ccgaggtccg cgagtggatc gcgcagcacg gcgactccgc tgtcgtggtg
     1081 ggcgacgacc ggctgatcaa ggtccatgtg cataccgagc agccgggcga gatcctgacc
     1141 tatgtctgcg cccgcggtaa cctgcgccag gtagccatca ccgatatgca ggagcagcac
     1201 gaggaattcc tcgacatgca tgagcaggcg gcggcgaaag ccccggcggc gcccgccgtc
     1261 cccgcgaccg gcgacccggc gctgagcaag atcgccaccg tcgccgtcgt gtcgggggcg
     1321 ggcctggccg agatcttccg cagcatgggc gcgaccgcgc tggtcgaggg cgggcagacg
     1381 atgaacccca gcaccgaaga actgctgcgc accatcgagg ccctgcccca cgacgaagtc
     1441 attatcctgc ccaacaacgg caacatcgtc atggccgcca agcagaccca ggcgctcagc
     1501 cggaagcgcg tcgccgtcgt gcccacggac accatcccgc aaggcatggc cgccctggtc
     1561 gccttcaatt acgaagccga cctggacacc aacgcccgcg ccatggatgc ggcggcccag
     1621 aatgtcgaga ccggcgaaat cacgaccgcc gtgcgccagg tgagcatgaa cggcatcgag
     1681 atccagatcg gcgacgtgat cggcttgcat aacggcaccc tggtcatcgc gggccactcg
     1741 aacgaggaag tcgcccacga tctgttggcg caactcaatg ccggggaccg cgaactgatc
     1801 acgatttatt atgggcagga cgtgaccgcc gagcaggccg agaccttcgc ggaccagatc
     1861 cgcgcgcagt atcccgacca ggaggtcgag gccctgtacg gcggccagcc gttttacgcc
     1921 tatattatct cggtggagta gacgcccgct cccgccgcct gaacggagtc tgacataccc
     1981 atgcccacga ttcgggtcgt caccgacagc acctgcgacc tacccgattc gcttgttcgc
     2041 gagtacggca tcaccgtcat cccggtcagt gtgcagatcg gatcgcacac ctatctggac
     2101 cgggtgaatc tgaccaccga cgagttgctg cgccacctgc aggcgggcga cccccggccc
     2161 acgacggcgc ccccggcggt cgccgcgttt gagcaggtct accggcaact ccaggccaac
     2221 cgcccctgcg acggcatcgt ctccatccat gtctcctcac ggctgagcgg cacctacaac
     2281 gctgcgctga tggcccgcga cgccttcctg aacagctcct tcccggttgg cgtgatggac
     2341 tcgcaggcgg ccagcatggg cctgggcctg gtggtgctgg ccgccgcccg caaagccgcg
     2401 gcgggcggca ccttgagcga ggtgatgaac gccgcccagc gcatgatcca ccaggtgcat
     2461 attgccttct t
//
LOCUS       DASUYC010000945         2072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1130507, whole genome shotgun sequence.
ACCESSION   DASUYC010000945 DASUYC010000000
VERSION     DASUYC010000945.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130507"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VF276_15445"
     CDS             <1..354
                     /locus_tag="VF276_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005511211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608294.1"
                     /translation="RPMAVLSGALNIIFLIAMIQAAARGLSPDCGCFAGAQDTVVGQM
                     VIQFFPPINWVMNEPVGVISIGRDIVFLLMSIHLFLVPGVFALDNWRHARQVALLDEE
                     ADAAGEEDGDPEPAV"
     gene            complement(446..>2072)
                     /locus_tag="VF276_15450"
     CDS             complement(446..>2072)
                     /locus_tag="VF276_15450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608295.1"
                     /translation="WSPDGTQIALVTEALTLYTMKADGSNLQRLADGVYSNPFWSPDG
                     RFIAYLGGEAWSAKPLPRGDLWIMPSGSGAAWKVPGATDIPSLPAGVAWSPDGTHIAA
                     GFPGHIFDVTDPAAAPAPLPGDGVKMWVLGGAWSPDGRYLAVSDGNRFGVLTLATGHF
                     NTVLSARGAPGTTKAGASWAPGPKRVVISVSGSSGDPHRVYSADLDGLNPKPIWTVPY
                     TYNPRNDEISSIGPPGVSPSGQQILIRVSRTKTQAGKLIFLHSTWLLQLDGSNNKELI
                     PSSFNAVWKPKVRFATDPAFYNTWKLSDNAVAAGWVARPWLWGPQPILSTKEPWSDSP
                     GGVRQVAYFDKGRMEIANPAAPRPDKYYVTSGLLARELVTGQIQVGPNTFTNTVPANV
                     VVTGDAANNPAPTYATFNKLGAGTDAGKTTDRTGRHVAATLFADGAVADDPGLGAGVT
                     YAHFVAESRHNIPDAFWDWLQKQPDWVALMGYPISEPYWVRATVAGKPDQPVLVQIFE
                     RRTLSFNFQNPPEWRVELGNVGQQYVAWRYPGK"
BASE COUNT          283 a          674 c          762 g          353 t
ORIGIN      
        1 cggcccatgg ccgtcctgtc gggcgcgctc aacatcatct tcctgatcgc catgatccag
       61 gcggcggcgc gcggcctgtc gcccgactgc ggctgctttg ccggcgccca ggacaccgtg
      121 gtgggccaga tggtcatcca gttcttcccg ccgattaact gggtgatgaa cgagccggtc
      181 ggcgtgatca gcatcgggcg cgatatcgtc ttcttgctga tgtcgatcca tctgttcctg
      241 gtgcccggcg tcttcgccct agacaactgg cggcatgccc gccaggtggc cctcctcgat
      301 gaggaagcgg acgcagccgg agaagaggac ggcgacccgg aaccggcggt ctgacagcgc
      361 gggccggtcg caacccggcc actacaacga tcaagaaagc ccccacgcgg cacctgccgc
      421 gtgggggctg cgtgtttgcc cggtgttact tgccgggata gcgccaggcc acgtactgct
      481 ggccgacgtt gcccagttcg acgcgccact ccggcgggtt ctggaagttg aagctgaggg
      541 tgcgccgctc gaagatctgc accagcaccg gctggtcggg tttgccggcc acggtagcgc
      601 gcacccagta gggttcgctg atcgggtagc ccatcagcgc gacccagtcg ggttgctttt
      661 gcagccagtc ccagaaggcg tcggggatat tgtgcctgct ctcggcgacg aagtgggcgt
      721 aggtcacgcc cgcgcccagg ccgggatcgt ctgcgacggc gccgtcggcg aacagcgtgg
      781 cggcgacgtg ccggccggtg cggtcggtgg ttttccccgc gtcggtgccc gcgcccagct
      841 tattgaacgt cgcgtaggtg ggggcggggt tgttcgccgc gtccccggtg acgacgacgt
      901 tggccggcac ggtgttggtg aaggtgttgg ggccgacctg aatctgcccc gtcaccagct
      961 cccgcgccag caagcccgat gtgacataat atttatccgg gcgcggggcc gcggggttgg
     1021 cgatctccat gcggcccttg tcgaagtatg ccacctggcg cacgccgccc ggcgagtcgc
     1081 tccacggctc cttcgtgctc aggatgggct gcggccccca cagccagggc cgggccaccc
     1141 agcccgcggc cacggcgttg tcgctcagct tccaggtgtt gtagaaggcc gggtcggtcg
     1201 cgaagcgcac cttcggcttc caaacggcgt tgaagctgct ggggatcagt tccttgttgt
     1261 tgctgccgtc gagttgcaag agccaggtgg agtgcaggaa gatcagcttg cccgcctggg
     1321 tcttggtgcg gctgacgcgg atgaggatct gctgcccgct gggcgagacg ccgggcgggc
     1381 cgatggacga gatctcgtcg ttgcgcgggt tgtaggtgta gggcacggtc cagatcggtt
     1441 tggggttcag cccgtcgaga tcggcgctgt agacgcggtg cgggtcgccg ctggacccgg
     1501 agacggagat cacgacccgc ttggggccgg gcgcccagga tgcgccggcc ttggtcgtgc
     1561 cgggcgcgcc gcgggcgctg agcaccgtgt tgaagtggcc cgtcgccagg gtcagcacgc
     1621 cgaagcggtt gccgtcgctc acggccaggt agcggccgtc gggcgaccag gcgccgccga
     1681 gcacccacat cttcacgccg tcgccgggca ggggcgcggg cgccgctgcc gggtcggtca
     1741 cgtcgaagat atggccgggg aagcctgccg cgatgtgcgt gccgtcgggc gaccaggcca
     1801 cgcccgcggg caggctgggg atatcggtcg cgccgggcac cttccaggcc gccccgctgc
     1861 cgctcggcat gatccagagg tcgccacgcg gcagcggctt ggcgctccac gcctcgccgc
     1921 ccaggtaggc gatgaagcgg ccgtcgggcg accagaaggg gttcgagtag acgccgtcgg
     1981 ccagccgctg caggttgctg ccgtcggcct tcatcgtgta gagggtgagc gcctcggtga
     2041 ccagggcgat ctgtgtgccg tccggcgacc ag
//
LOCUS       DASUYC010000946         4767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_2005809, whole genome shotgun sequence.
ACCESSION   DASUYC010000946 DASUYC010000000
VERSION     DASUYC010000946.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4767
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2005809"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VF276_15455"
     CDS             <1..286
                     /locus_tag="VF276_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX6608296.1"
                     /translation="VEIFTTAARQAGVPLAAAAAGSMWGFFCREGAPRNFAEAKQADT
                     ARYARLFHALLDAGVYLAPSQFEAAFVSTAHDAGTLEHTARAVAAALRAA"
     gene            606..2210
                     /locus_tag="VF276_15460"
     CDS             606..2210
                     /locus_tag="VF276_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEX6608297.1"
                     /translation="MKLDDYPYPSQRMPVFAPRGVVATGQPLAAQAGLNMLQQGGNAC
                     DAALATAIALTVVEPTSNGIGSDAFALLWDGQTLHGLNGSGRACAAATIEAVRAAGHT
                     QMPARGWMPVTVPGAPATWRDLHARFGRLPFATLFEPAIYYAEHGYPVSPMTAAGWGR
                     AAQLYAQDSSPALAGWAATFAPNGRAPRTGEIWASPGHARTLRRIAESRAEDFYQGEI
                     AQAIAGFARDTGGWMTADDLACHTCTWVDPISTNYHGYDVWEIPPNGQGITTLIALNI
                     LSNFDLGPLGRESVESYHLQIEALKLAYADALAYVADPDHCSVPVRELLSRDYARERA
                     ALIDNDALLPAAGQPLRGGTVYLCAADSDGMMVSLIQSNYMGFGSGVVVPGTGVALHN
                     RGHDFRLDPTHPNHLRPGKRPYHTIIPGFLTRDGQAVGPFGVMGGYMQPQGHLQMVLN
                     MVDYGLNPQASLDAPRWQWTQGRTVLLEDEVPRPILEGLALRGHEVSVEPVGPAFGRG
                     QIIRRLPGGAYVAGSEKRADGAAAGY"
     gene            2267..3271
                     /gene="glk"
                     /locus_tag="VF276_15465"
     CDS             2267..3271
                     /gene="glk"
                     /locus_tag="VF276_15465"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEX6608298.1"
                     /translation="MMLLAGDIGGTKTELALFAPETGPREELKCAEFPSGRYPSLEAI
                     VQEFLAGVDKPIDRAVFAVAGPVIAGQAHVTNLDWRVNLAHLQETLNLPNVTLMNDLQ
                     AIALAVPILTPDEVVTLNEGVAQPHGTIAVIAPGTGMGEAFLVWNGTRYQALASEGGH
                     SDFAPGNATEIGLLQYLQPRFGHVSVERVCSGRGLPNIYDYLRDRDPAQETPEVARQL
                     AEAEDRTPVIVKNALDSSRSCSLCVATLDIFIAILGAEAGNLALKMLSTGGVYLAGGI
                     PPRILPLLQNGRFMQAFMRKGRLSELLAGMPVRVICSRAALLGAAHYGLDIAGDKRPV
                     "
     gene            complement(3288..4262)
                     /locus_tag="VF276_15470"
     CDS             complement(3288..4262)
                     /locus_tag="VF276_15470"
                     /inference="COORDINATES: protein motif:HMM:NF023218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX6608299.1"
                     /translation="MRRPTLRDLILIALAGLWLLVLAVLVLRPAAPAPIRQFPPTPVP
                     ATPTATPGPQLAPLPRGYGVAVAGDRGSRADLDLLGPVWYYDYHAKGADWGSHLRILM
                     VRPADKLSDAELRHLAAIHPGHWWMLGNEPNDPAQDNQDPAAYARFYAHTAAVIRSAD
                     PAAGIMPAGIANADAQWAAAFRTQYERMTGAPPQVDAWNIHNYLLDTADPYDLGEFQR
                     RIGKFRAWMAAVGDGDRPLVLSEFGVRYGAGCCNRPLDVLGDGLVFMRRAITSLEESG
                     QVDSWAWFTLDSGDLDYDGNLLRPAVPGTLSPYGRLYSDHARMFALPR"
     gene            complement(4438..>4767)
                     /locus_tag="VF276_15475"
     CDS             complement(4438..>4767)
                     /locus_tag="VF276_15475"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6608300.1"
                     /translation="QVVIFHNGTQEERNIPVDAVLLNLGFKADIGPIKEWGLQLDKRT
                     IVINERTETNLPGVYAAGDIAAEAVKMNLIATCYGQAALAVNVAKNYIDPRSSIFPGH
                     SSEKMGH"
BASE COUNT          736 a         1624 c         1645 g          762 t
ORIGIN      
        1 ggtcgagatc ttcaccacgg cggcccggca ggcgggcgtg ccgctggctg ccgccgccgc
       61 gggcagcatg tggggcttct tctgccgcga gggcgccccg cggaacttcg ccgaagccaa
      121 gcaggccgac accgcgcgct acgcccgtct cttccacgcc ttgctggatg cgggcgtgta
      181 cctggcaccc tcgcagttcg aggccgcgtt cgtcagcacc gcgcatgacg ccggaacgct
      241 ggagcacacc gcccgtgccg tggccgcggc gctgcgggcc gcctgacgcc ggtccgaaaa
      301 actggggaaa atcgatctca aaactcgtca aaattggtcc tatccttttt tagcccccgg
      361 cgttatgaat accggaactc ggacgcaagt gtctgtatcc agagcacagt cgatatgtcg
      421 ggaggacgcg cccccactcg gtatatggac tcctctgtat cagcgcacgg tgcccaccgg
      481 agcaacccca agtggttgtt ccggtttttt ttgccctgcg tgcccgcggc gggccgtgac
      541 cgggattctt tgctataatg cagcgaagct attcgcatcg cgatgcgccc gcaggaggcc
      601 cggccatgaa actcgacgac tatccctatc cctcgcagcg tatgcccgtc tttgccccgc
      661 gcggcgtggt ggcgaccggc cagccgctgg cggcccaggc cggcctcaac atgttgcagc
      721 agggtggcaa cgcctgcgac gccgccctgg cgacggccat cgcgctcact gtcgtggagc
      781 cgacctcgaa cggcatcggc agcgatgcct tcgccttgct atgggacggg cagacgctgc
      841 acggcctgaa cgggtcgggg cgggcctgcg cggcggcgac gatcgaagcg gtgcgcgccg
      901 ccgggcacac gcagatgccg gcgcgcggct ggatgcccgt gaccgtgccg ggagcgcccg
      961 cgacctggcg cgatctgcat gcgcgtttcg ggcgcctgcc cttcgccacg ttgttcgagc
     1021 cggccatcta ctacgccgag cacggctacc ccgtgtcgcc catgacggcg gcgggctggg
     1081 gccgggcggc gcaactgtac gcgcaagact cgtcgccggc gctggccggg tgggcggcca
     1141 ctttcgcccc caacgggcgc gcaccgcgca ccggcgagat ctgggccagc cccggccacg
     1201 cccgtaccct gcgccgcatc gccgagagcc gggccgagga tttctaccag ggtgagatcg
     1261 cccaggctat cgcgggattt gcgcgcgaca ccggcggttg gatgacggcg gacgacctgg
     1321 cctgccatac ctgcacctgg gtcgatccga tcagcacgaa ctatcacggc tacgatgtct
     1381 gggagatccc gccgaacggg cagggcatca cgaccctcat cgcgctgaac atcctcagca
     1441 acttcgacct cggcccactg gggcgcgagt cggtggaaag ctaccacctg caaatcgagg
     1501 cgctgaaact ggcctacgcc gacgccctgg cctacgtcgc cgaccccgat cactgctcgg
     1561 tgccggtgcg cgaactgctg agccgggact acgcccgcga gcgcgccgcg ttgatcgaca
     1621 acgatgcact actacccgct gccgggcagc ccttgcgcgg tggcaccgtc tacctgtgcg
     1681 ccgccgacag cgacggcatg atggtcagcc ttatccagtc gaactacatg ggctttggat
     1741 cgggcgtggt cgtgcccggc accggggtcg cgctgcacaa ccgcggccac gatttccgcc
     1801 tcgaccccac ccacccgaac cacttgcgcc ccggcaagcg gccctatcac acgatcatcc
     1861 ccggcttcct gacccgcgac ggccaggccg tggggccgtt cggcgtgatg ggcggctaca
     1921 tgcagccgca gggccacctg cagatggtcc tcaacatggt ggactatggc ctcaacccgc
     1981 aggccagcct cgacgccccg cgctggcagt ggacccaggg gcgcaccgtg ctgctggagg
     2041 acgaggtgcc gcgccccatt ctcgaagggc tggccttgcg cggccacgag gtgagcgtcg
     2101 agccggtggg cccggcgttc gggcgggggc agatcatccg gcggctgccg ggcggcgcct
     2161 atgtcgccgg cagcgagaag cgcgccgacg gtgccgccgc cggctattaa ccaacggccc
     2221 gcaacccgcc ccgccgcggg gccgggcagc ggatacagga ggatgtatga tgttactggc
     2281 aggggatatc ggcgggacca aaacggagct ggcgctcttc gcgcccgaaa ccgggccacg
     2341 ggaggagctt aagtgcgccg agttccccag cgggcgctat ccctcgctgg aagccatcgt
     2401 ccaggagttt ctcgccggcg tggataagcc catcgaccgg gccgtgttcg ccgtggccgg
     2461 gccggtaatc gccgggcagg ctcacgttac gaatctggac tggcgggtga atctggctca
     2521 tctgcaagag acgctcaacc tgcccaacgt cacactgatg aacgatctgc aggccatcgc
     2581 tctggccgta ccgatcttga cgccggacga agtcgtcacc ctcaacgagg gcgtggccca
     2641 gccgcacggc accatcgcgg tgatcgcgcc gggcaccggc atgggcgaag ccttcctggt
     2701 ctggaacggc acccgctacc aggccctggc gtccgagggc ggccattccg atttcgcgcc
     2761 cggcaacgcg accgagatcg gcttgctcca gtacttgcag ccgcggttcg ggcacgtcag
     2821 cgttgagcgg gtctgctccg ggcgcgggct gcccaacatc tacgactacc tgcgcgaccg
     2881 cgacccggcc caggaaaccc cggaggtggc ccggcaactg gccgaggcgg aggaccggac
     2941 gccggtgatc gtgaaaaacg ccctggactc ctcgcgctcc tgctcgctct gtgtcgccac
     3001 cctcgacatc ttcatcgcga tcctgggcgc cgaggcgggg aacctggccc tcaagatgct
     3061 ctccacgggc ggcgtgtacc tggcgggcgg aatcccgccc cgtatcctgc cgctgctgca
     3121 gaacggccgc ttcatgcaag cattcatgcg caaaggtcgc ctgtcggagc tgctggccgg
     3181 tatgccggtg cgcgtcatct gttcgcgcgc tgccctgctg ggcgccgccc actacgggct
     3241 ggatatcgcc ggggacaagc ggcctgtcta gcgcggcgcg ggccgtgtta gcggggcagc
     3301 gcaaacatgc gcgcgtggtc gctgtagagc cgcccatagg gtgagagcgt gccggggacg
     3361 gcggggcgca gcaagttgcc gtcgtaatcc aggtcgccgc tgtcgagcgt gaaccaggcc
     3421 caggaatcga cctgtccgct ctcctccagc gaggtgatcg cgcggcgcat gaacaccagc
     3481 ccgtcgccca gcacgtcgag cgggcggttg cagcaccccg cgccgtagcg cacgccgaac
     3541 tcgctgagca ccaggggccg gtcgccgtcg cccaccgccg ccatccaggc ccggaacttg
     3601 ccgatgcgcc gctggaactc gcccaggtca taggggtccg ccgtgtcgag caggtagtta
     3661 tggatgttcc aggcatcgac ctggggcggc gcgccggtca tccgctcgta ctgggtgcga
     3721 aaagccgcgg cccactgcgc gtcggcattg gcgatccccg ccggcatgat gcccgccgcc
     3781 gggtcggcgg agcggatcac ggcggcggtg tgggcataga agcgcgcgta ggcggcaggg
     3841 tcctggttgt cctgggccgg gtcgttgggc tcgttgccca gcatccacca gtggccggga
     3901 tggattgcgg ccaggtgccg tagttcagca tcggagagct tgtcggcagg gcgcaccatc
     3961 aggatgcgca gatgcgatcc ccaatcagcc cccttggcgt ggtagtcgta gtaccagacc
     4021 ggccccagca gatcgaggtc cgcgcggctg cccctatcgc cggccaccgc cacgccatag
     4081 ccgcgcggga gcggtgcaag ctgcgggccg ggcgtggccg tcggggtggc ggggacgggc
     4141 gtgggcggga actgccggat cggcgccggc gccgcggggc gcaggacgag cacggccagg
     4201 accagcagcc agagtccggc cagcgcgatc aggatcaggt cacggagggt tgggcggcgc
     4261 ataggcgtgg tctgtcgtcg gtgggtcgtg atcggcgaac ggcgttcgca ggtgtattat
     4321 acccgctatg ggccgttatt acctagaagg ggtgggcctt gtagcagggc gggcgggtag
     4381 aatcagagcg cgcaggcgaa ttcgcctgcg cgctctgatt ctaagcgggt ggggcgccta
     4441 atggcccatc ttctcgctgc tgtggccggg gaagatgctg gaacgcgggt cgatgtaatt
     4501 cttcgccacg ttcacggcga gcgccgcctg gccgtagcag gtggcgatga ggttcatctt
     4561 caccgcttcc gccgcgatat cgcccgctgc gtagacgccg ggcaggttgg tctcggtgcg
     4621 ctcgttaatg acgatggtgc gcttgtcgag ttgcaggccc cattccttga tcgggccgat
     4681 atcggccttg aagcccaggt tgagcagcac ggcatcgacc gggatgttgc gctcttcctg
     4741 cgtgccgtta tggaatatca ccacctg
//
LOCUS       DASUYC010000947         3450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_902923,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000947 DASUYC010000000
VERSION     DASUYC010000947.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902923"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            428..2116
                     /locus_tag="VF276_15480"
     CDS             428..2116
                     /locus_tag="VF276_15480"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX6608301.1"
                     /translation="MATSFADLQLSPGLLETVAELGYEEPTPIQEQTIRLLIEGRDVI
                     AQAQTGTGKTAAFAIPIVECVDPDDRHVQALVMAPTRELAVQVAEATHRLGRSKGLSV
                     LPVYGGQPIHRQLHVLARGVQVVIGTPGRLLDHLRRGTLDLAHVKYVILDEADEMLDM
                     GFIEDIESILDNVPENRQTALFSATIPPRIAALAQQYLRDPVRITIAPTELTIPAIEQ
                     YYVEVTPRNKLDSLTRILDHDEPESAIIFARTKRDVDELGEALQSRGFAAETLHGDLN
                     QTQRDRVMNRFRGNQVELLVATDVAARGLDISGVTHVFNYAIPEDAEAYVHRIGRTGR
                     AGKTGKAITFVQPNEIRLLRIIQRIIGQKINPLRLPTLADVEARRREALKATLRERIT
                     QGNLTPYLDLVTELAEEFDLAEIAAAAANMASAGDRPLTEVVEVSNSSDGVEPGMARL
                     FLNVGRQAGVRPGDIVGAIANEAGIPGRAIGAIDIYDTTAFVEVPAADRERVMRALSH
                     TTIKGQRVQVAVAQGELPARPPRPTGPPTGFRGGHHRATHKGHGPGKYGKQRHK"
     gene            complement(2136..2690)
                     /locus_tag="VF276_15485"
     CDS             complement(2136..2690)
                     /locus_tag="VF276_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
                     /protein_id="HEX6608302.1"
                     /translation="MAGEFAPLLGVLLVLTLLVGGGALIAARRMQGRSGLPRARVVYT
                     DAGAWQRATDSLFSRRYGLVGKPDYLLEEQGRIIPVEVKPTRRATQPYPGDVLQLLAY
                     CLLVEDTQGRPGYGLLRYAENTFRIDYTDAARAELLATLDEIRTARLAGELDRSHDDP
                     VRCANCMFATVCDQSLAPASPRRG"
     gene            complement(2680..>3450)
                     /locus_tag="VF276_15490"
     CDS             complement(2680..>3450)
                     /locus_tag="VF276_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEX6608303.1"
                     /translation="RAMRPNTRVVYTESPTNPTMDLTDLRATAEIAHAGGALAITDNT
                     FASSYNQHPLDLGYDLVVHSATKYLNGHADVTAGAIMGSRARLDAAWEYLRVYGPVLH
                     PFEAWLLRRGLLTYPLRMAAHNRNALAVARFLAEHPAVAHVYYPGLPSHPQHELAQRQ
                     MPGGFGGMMAFELKGGYDAGYRMIGRTKVCVLAVSLGGVETLITHPASMIHTFQSEEQ
                     KAGAGISPGLIRLSVGIEEVEDIIADLDQALAVATGGG"
BASE COUNT          576 a         1139 c         1237 g          498 t
ORIGIN      
        1 ggcggtggtg tccgtagggg ctacggcgac cgccgtggcc gtgggcggga cgggggtcgg
       61 ggtgggcgtg gcctcggggc cggcaaacgg attgcaggcc gtgagcaggc ataaaatgag
      121 taaaaagagg gggattcggc ggcgcatggc gggctcctgt ctgcgggtgc ccggttgcac
      181 ccgtggctat gctttaatat agcggagccg ggctgccgaa tcaaggcgac gccgccccca
      241 cgggacggtc tcacgtgctc cggggctctg tgcgccccgt caggcaaggg aggggtcgca
      301 ctcaggacgc gattcagcac attccgggct gcactatagt tatatctatc tggttagtga
      361 acaaagaatc cgtggctgat gggcgggaat ctacccggtg cgcggatctg gataaggagc
      421 aagaggcatg gcaaccagtt ttgccgattt acaattaagc cccggcctgc tcgaaacggt
      481 ggccgagctg ggctatgagg aacccacgcc cattcaggag cagacgatcc gcctgctgat
      541 cgagggccgc gacgtgatcg cccaggcgca gaccggcacg ggcaagacgg cggctttcgc
      601 catcccgatt gtcgagtgcg tggaccccga cgaccgtcac gtgcaagccc tggtcatggc
      661 gccgacccgc gaactggcgg tgcaggtggc cgaagcgacg caccgcctgg gccgctccaa
      721 gggtctcagc gtgctgcccg tctatggggg gcagccgatc caccggcaat tgcatgtgct
      781 ggcccgcggt gtccaggtgg tgatcggcac gccgggccgc ctgctcgatc acctgcggcg
      841 gggtacgctc gacctggccc acgtcaagta tgtgatcctg gacgaggccg acgagatgct
      901 cgacatgggc ttcatcgaag acatcgagag catcctcgat aatgtgcccg agaaccgcca
      961 gacggcgctg ttctcggcga ccatcccgcc gcgcatcgcc gcgctggccc agcagtacct
     1021 gcgcgacccg gtgcgcatca ccatcgcccc gaccgagttg accatcccgg ccatcgagca
     1081 gtactatgtc gaggtcacgc cgcgcaacaa actcgactcg ctgacgcgca tcctcgacca
     1141 tgacgaaccg gaatcggcca tcatcttcgc ccgcaccaag cgcgacgtgg acgaactggg
     1201 cgaggcgttg cagtcgcgcg gcttcgccgc cgagacgctg cacggcgacc tgaaccagac
     1261 ccagcgcgac cgggtgatga atcgcttccg cggcaaccag gtcgagctac tggtcgccac
     1321 cgacgtggcg gcgcgcgggc tcgacatcag cggcgtgacc cacgtgttca actacgccat
     1381 ccccgaggac gctgaggcgt atgtccaccg catcgggcgc accgggcgcg cgggcaagac
     1441 gggcaaggcg atcaccttcg tgcagcccaa cgagattcgc ctgctgcgca tcatccaacg
     1501 catcatcggg cagaagatca acccgctgcg cctgcccacg ctggccgacg tggaggcccg
     1561 ccgccgcgag gcgctgaagg cgacattgcg cgagcgcatc acccagggca acctcacgcc
     1621 ctacctggac ctggtgacgg agctggccga ggagttcgat ctagccgaga tcgccgcggc
     1681 ggcggccaac atggcgagcg cgggcgaccg gccgctgacc gaggtggtgg aggtcagcaa
     1741 cagcagcgac ggcgtggagc cgggcatggc ccgcctgttc ctgaacgtcg ggcggcaggc
     1801 gggggtgcgc cccggcgaca tcgtcggcgc catcgccaac gaggcgggca ttccggggcg
     1861 ggccatcggc gccatcgaca tctacgacac cacggcgttt gtcgaagtgc ccgctgccga
     1921 ccgggaacgg gtcatgcgcg cgctgtcgca cacgacgatc aagggccagc gcgtgcaggt
     1981 ggcggtggcg cagggcgaac tgccggcccg cccgccccgg cccaccggcc cgcccaccgg
     2041 ctttcgcggc ggccaccacc gcgccacgca caagggccac gggccgggca agtacggcaa
     2101 gcagcgccac aagtagccgg aatagcgcgg gggggttagc cccggcgcgg ggaggcgggg
     2161 gccagcgatt gatcacagac cgtggcgaac atgcagttgg cacagcgcac cgggtcgtca
     2221 tggctgcggt cgagttcgcc cgcgagccgg gcggtgcgga tctcgtccag cgtggcgagg
     2281 agttcggcgc gggcggcgtc ggtgtagtcg atgcggaagg tgttctcggc gtagcgcagc
     2341 aggccgtagc cggggcggcc ctgggtgtcc tcgaccagca ggcaataggc gaggagttgc
     2401 agcacgtcgc cggggtaggg ctgggtggcc cggcgggtcg gcttgacctc gaccgggatg
     2461 atgcggccct gctcttccag caggtagtcg ggcttgccca ccagcccata gcggcgcgag
     2521 aaaagcgagt cggtggcccg ctgccaggcc ccggcgtcgg tgtagaccac gcgggcgcgc
     2581 ggcaggccgc tgcgtccctg catccgccgg gcggcgatca gcgcgccgcc acccaccagc
     2641 aaggtgagca ccagcaagac gcccagcagc ggcgcgaact cacccgccac cggtcgccac
     2701 cgccagcgcc tggtcgagat cggcgatgat gtcctccacc tcttcgatgc ccaccgacag
     2761 ccggataagg cccggcgaga tgcccgcgcc cgccttctgc tcctcgctct ggaaggtgtg
     2821 gatcatcgac gccgggtggg tgatcaacgt ttcgacgccg cccaggctca ccgccaggac
     2881 gcagaccttc gtgcgcccga tcatgcggta gccggcgtcg tagccgccct ttagctcgaa
     2941 ggccatcatg ccgccgaagc cgcccggcat ctggcgctgt gccagttcgt gctgcgggtg
     3001 cgacggcagg ccgggataat agacgtgggc gacggcgggg tgctcggcca ggaagcgggc
     3061 cacggccagc gcgttgcggt tgtgcgcggc catgcgtagc ggataggtca gcaggccccg
     3121 gcgcaggagc caggcttcaa acgggtgcaa gaccggcccg tacacgcgca aatactccca
     3181 cgccgcgtcg agccgcgctc tgctgcccat gatcgccccg gcggtcacgt cggcgtggcc
     3241 gttcaggtat ttcgtggcgc tatggaccac cagatcgtag cccaggtcga gcgggtgctg
     3301 gttgtaggac gaggcaaagg tgttgtcggt gatcgccagc gccccgccgg catgggcgat
     3361 ctcggcggtg gcgcggagat cggtcaggtc catcgtgggg ttggtgggcg actcggtgta
     3421 gacgacgcgc gtgttggggc gcatggcgcg
//
LOCUS       DASUYC010000948         4247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_619358,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000948 DASUYC010000000
VERSION     DASUYC010000948.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619358"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VF276_15495"
     CDS             complement(<1..208)
                     /locus_tag="VF276_15495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608304.1"
                     /translation="MEQLARRLTRAWYERGVIALLVLGAGFCLAIYLLAGINLGVDLP
                     ALGRLLMRLALPPLMLCPVVFAGLL"
     gene            complement(521..1678)
                     /locus_tag="VF276_15500"
     CDS             complement(521..1678)
                     /locus_tag="VF276_15500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX6608305.1"
                     /translation="MTVSYWQDRSGEQTLTCDTCVVGAGMVGAYTARCLVAAGEDVVL
                     VEARHVAAGATGRNAGMVLTGLAAYYHEAVEQYGPDVAREVWQLTLENRALMFGLAAE
                     YGVPHEQNGSLVLALDGPEAAALERTAAALAAEGLPGDFGHGDPTGRGFTAAIVQPGD
                     GATHPARLAEALVRAGGARLLENSEVFAIRPLGDGLEVRSRLAAIHCRRVALCTNAYA
                     PLLHPFFAGLVAPTRGQVFVTAPVAPRVLDRPGYADWGYEYFRQLPDGAFLLGGWRHH
                     FRAEEIGYEDRVTPGVQAGLARFLARYFPELADVPITHRWSGVMGFSPDHIPLVGLVP
                     DLPGVGFAVGFTGHGMGWGLKTAERLVALLLHGTSPGILDARRLATRPAAV"
     gene            2067..3260
                     /locus_tag="VF276_15505"
     CDS             2067..3260
                     /locus_tag="VF276_15505"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX6608306.1"
                     /translation="MKLIVQIPCHNEEQALPVTIPTIPRRIPGVDAVEILIIDDGSQD
                     RTVAVARSLGVDHIVRHTSNHGLAAAFQTGLDACLRLGADIIVNTDADNQYPQDRIPD
                     LIAPILRQEADMVIGDRQVAEIEHFSPIKKYLQGFGSRVVRAVSGTDVPDAPSGFRAF
                     SREAALRLQVITQYTYTLETIIQAGKKNLAVGHVPVRTNPMLRKSRLVRSIPDYVKKS
                     MTTIVRIWALYEPLKIFTYIGGIFIFIGLWGVFWLLYHWAIDENFNWFRTFTGHLPTT
                     FAAVLGFVFGIQIFLIGLVADILAASRRLTEETLYRVKRMDLELAELQRRGQTLVTTL
                     QEHGLDGAAAITATETPDVHPAPDAASPPPEYIPNGVTSPAGLDTAIDTTVDPARRPD
                     SPSTS"
     gene            3527..3883
                     /locus_tag="VF276_15510"
     CDS             3527..3883
                     /locus_tag="VF276_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX6608307.1"
                     /translation="MLEWLAVLLFVTICLANAVLLGNISRIVGPHRPTAAKLTPYESG
                     MPPIGPAHQRYTVRYYLIAMLFVIFDIEAVFLYPWAIAYNAIGIYGLVEMILFLLLLI
                     VGYVYAWRKGAFDWAS"
     gene            3871..>4247
                     /gene="nuoB"
                     /locus_tag="VF276_15515"
     CDS             3871..>4247
                     /gene="nuoB"
                     /locus_tag="VF276_15515"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HEX6608308.1"
                     /translation="MGVLENRFKPGVITTTADFLFNWAHRSSMWPLILGTACCAIEMM
                     AVGISRFDMLERFGMLYRFSPRQSDVMLVCGTVTKKFAPVMRTVWDQMAEPKYCIAVG
                     NCASAGGPFPTYAVVQGIDEVVPV"
BASE COUNT          754 a         1444 c         1317 g          732 t
ORIGIN      
        1 tgagcaaccc cgcgaacacc accgggcaca acatcagcgg gggtaacgcc agccgcatga
       61 gcaggcgccc caacgccggc agatccacgc cgagattgat gccggccagc agatagatcg
      121 ccaggcagaa accggcgcct agaacgagca gcgcgatgac ccctcgttca taccaggcgc
      181 gggtgagccg tctcgccaac tgttccatcg acgtctaccc cgtaaggatc agcggcgcgc
      241 gtgtttcccc atggggccac caagcggcac caggggcacg ccgtgaccat cggctgcaaa
      301 tacttcactg gagccccgcc cgcccaggac gccgcgcagc cggcggcgtg aggaggtggg
      361 tccattatag cacgagtgaa ttttcgcccg ccacaattct gtacaatatg taggattctt
      421 tgtaagtagg gcttgccgtt tttgcggtgc atcaggatca catgcgaaaa gcgctgaagg
      481 ggtcgcctcg acaggcggcc cccggtggcg gtcccccggc ttaaactgcc gcaggccggg
      541 tggccaggcg ccgcgcatcg aggatgccgg gcgaggtgcc gtgcagcagc agggccacca
      601 gccgttcggc ggtcttgagc ccccagccca tgccgtgccc cgtgaagccg acggcgaagc
      661 ccacccccgg caggtcgggc accagcccca ccagcgggat atggtcgggt gaaaagccca
      721 tcacgcccga ccagcggtgc gtgatgggta cgtccgccag ttcggggaag tagcgggcca
      781 ggaaccttgc gagtccggcc tgcacacccg gcgtcacccg gtcctcgtag ccgatctcct
      841 cggcgcggaa gtggtggcgc cagccgccca gcagaaacgc gccgtcgggc agttggcgga
      901 aatactcata gccccagtcg gcgtaaccag gccggtcgag cacccgcggg gccaccggcg
      961 ccgtgacgaa cacctggccg cgcgtcggcg cgacgagtcc ggcgaagaag gggtgcagca
     1021 gcggcgcata ggcgttggtg cagagggcca ctcggcggca gtgaatcgcc gccagccgcg
     1081 agcgcacctc caacccgtcg cccagcgggc ggatggcgaa gacctcgcta ttctccagca
     1141 ggcgcgcgcc gcccgcccgc accagcgcct cggccagccg cgccgggtgg gtggcgccgt
     1201 cgcccggctg gacgatcgcc gccgtgaagc cgcgcccggt cgggtcgccg tgcccgaagt
     1261 cgccgggtag cccctccgcc gccagcgccg ccgccgtgcg ttccagcgcc gccgcctccg
     1321 gcccgtccaa cgccagcacc agcgatccgt tttgttcgtg cggcacaccg tactcggcgg
     1381 ccaggccgaa catcagcgcc cggttctcca gcgtgagctg ccagacctcg cgggcgacat
     1441 cggggccgta ttgctcgacc gcttcatgat agtaggcggc cagcccggtc agcaccatgc
     1501 ccgcgttgcg ccccgtggcg cccgccgcga cgtgccgcgc ctcgaccagc accacgtcct
     1561 ccccggcggc caccaggcag cgcgcggtat acgcgccgac catgcccgcg ccgaccacac
     1621 aggtatcgca ggtcagcgtt tgctccccgc tgcgatcttg ccagtaagaa accgtcatcg
     1681 cgcacaaccc tcctcgcgag cagtatagca cagccgccaa acgagtgctg agtgctgagt
     1741 gctgagtgct gagcggttgt gaaacaaacc cccaggcacg aaatggggga ggtccaagcc
     1801 acaagatgtt gtggttatgt gggtgtacta accactacct gtcgtggtcc cggtagcacc
     1861 ggagggtttg tttcacagcc tctgagtgct gagtacgagg agggtggcgg agctggaagg
     1921 gggagagtgc tacgcggtcc gctcccctct ccgcggcggg gaggggctgg gggtggggtt
     1981 ggccaggcgt gcgcgcttga cccatccggc ctgacagagt acaatgccag caggccgggc
     2041 atttgtgccc gccggagagt attgttatga agctgatcgt tcagataccc tgccacaacg
     2101 aagaacaggc gttgccggtg accatcccga ctatcccgcg ccggattccc ggcgtggacg
     2161 cggtcgagat cctgatcatc gacgacggca gccaggaccg cacggtcgcg gttgcccgca
     2221 gcctgggcgt cgaccacatc gtgcgccaca cgtcgaacca tgggctggcc gccgctttcc
     2281 agaccggcct cgacgcctgc ctgcggcttg gcgccgacat catcgtcaat accgacgccg
     2341 acaaccagta cccgcaggac cgtattcccg atctcatcgc ccccatcctg cgccaggaag
     2401 ccgacatggt gatcggcgac cgccaggtgg ccgagatcga gcacttctcg ccgatcaaaa
     2461 agtatctgca agggttcggg tcgcgggtgg tgcgcgcggt gtcgggcacc gacgtacccg
     2521 acgcgcccag cggcttccgc gccttctcgc gcgaggctgc gttgcgcctg caggtcatca
     2581 cccagtacac ctacacgctg gagaccatta tccaggcggg caaaaagaac ctggctgtgg
     2641 gccacgtgcc cgtgcgcacc aaccccatgc tgcgcaaatc gcgcctggtg cgctccatcc
     2701 ccgactacgt caagaagtcg atgaccacca tcgtgcgcat ctgggcgctg tatgagccgc
     2761 tcaagatctt cacctacatc ggcggcatct tcatcttcat cgggctgtgg ggggtcttct
     2821 ggctgctcta tcactgggct atcgacgaga atttcaactg gttccgcacc ttcaccggcc
     2881 acctgcccac gaccttcgcc gctgtgctgg gcttcgtttt cggcatccag atcttcctca
     2941 tcggcctggt ggccgacatc ctggccgcca gccgccgcct gaccgaagaa acgctctatc
     3001 gcgtcaagcg catggatctg gaactggccg agctacagcg ccgtggccag acgctggtta
     3061 ccacgctgca agagcatggg ctcgacggcg ctgccgcaat caccgcgacc gagacacccg
     3121 atgtgcatcc cgcgcccgat gccgcgtccc ccccacccga atacatcccg aacggcgtga
     3181 catctcctgc cggactcgac accgccatcg acaccaccgt tgaccctgcc cggcggcccg
     3241 attcgccctc gacatcctga ccgtcccgcg gctgcgatgt ggcgtgccat ggcctaatac
     3301 ttgctgaccg caaaccagcg gagaactgcg ttcgtatata ttgtgataac tataacaatc
     3361 tttccgcatt aaaatcatcg gagtggtcca gtgacgccgc ggggtcggcc tctttgtagg
     3421 ggacaccccg aaaatgggca attgtcactg gaaaaacatt gtgatataat acacgctgtt
     3481 ttaggcggcg gaactaactt cccgccccgc cgcaaggagg caacggatgc tcgaatggct
     3541 cgcggtcttg ctctttgtca ccatttgcct ggcgaacgcg gtgttattgg gcaacatcag
     3601 ccgcattgtc ggtccgcacc ggcccacggc ggcgaaactc acaccttatg agtcgggcat
     3661 gccgcccatc ggccccgcgc accagcgcta caccgtgcgt tactatctga tcgccatgct
     3721 ctttgtcatc ttcgacattg aagccgtgtt cctctatccc tgggccattg cctataacgc
     3781 gattgggatt tatggcctgg tggaaatgat cctgttcctg ttgttgctca tcgtgggcta
     3841 cgtgtacgcg tggcggaagg gagcattcga ttgggcgtct tagaaaatcg ttttaagccg
     3901 ggcgtgatca ccaccacggc cgacttcctg ttcaactggg cacatcgctc gtcgatgtgg
     3961 ccgctgatcc tgggcaccgc ttgctgcgcc attgaaatga tggcggtggg catctcgcgc
     4021 ttcgacatgc tggagcgctt cggcatgctc taccgcttct cgccgcgcca gagcgacgtg
     4081 atgctcgtct gcggcaccgt caccaagaag ttcgcgccgg tcatgcgcac ggtctgggac
     4141 cagatggccg agccgaagta ttgcatcgcc gtgggcaact gcgcgtcggc cgggggtccg
     4201 ttcccgacct atgccgtggt gcagggcatc gacgaggtgg tgccggt
//
LOCUS       DASUYC010000949         3678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1124314, whole genome shotgun sequence.
ACCESSION   DASUYC010000949 DASUYC010000000
VERSION     DASUYC010000949.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124314"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VF276_15520"
     CDS             <1..502
                     /locus_tag="VF276_15520"
                     /inference="COORDINATES: protein motif:HMM:NF015559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HEX6608309.1"
                     /translation="LGIEALSRGAGWCDFVEQNAGVGRIIAANLAFTRLAERARVHSM
                     AVDAYLKLRAAGRSLLPGDKDPLPAVKCDIIFMDPPYADPAIREILGQVAAGPFLAPD
                     GLLVVGHATTLELPDAPGPLRRIRHRTFGGSAFSLYKYAAAAGSEADPGDADAAPDED
                     DDEETC"
     gene            503..1033
                     /gene="coaD"
                     /locus_tag="VF276_15525"
     CDS             503..1033
                     /gene="coaD"
                     /locus_tag="VF276_15525"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX6608310.1"
                     /translation="MRIAIYPGTFDPITNGHLDIARRAGLLFDKLIIAVGENPQKKPI
                     FTVEERMALIREAVREFDHSDIEVDSFSGLTIDYAKEIGAQAIVRGLRVLTDFEWELQ
                     LALINEKLAPEIETVCLMTSQEHSFLSASIVRELALHHSPKLRELVPPHVAAALDAVY
                     GVGAPSTAGGSDRSDV"
     gene            1086..1523
                     /locus_tag="VF276_15530"
     CDS             1086..1523
                     /locus_tag="VF276_15530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608311.1"
                     /translation="MDLQYLIDRLETMVRAGRRVPGLNRLMLDEQELIDLIDQMRTVL
                     PEEIRYAKHVLREKEQILSDAQAQADEILRTARQQAEVLIEREGLLKQARAQAEDIKR
                     EAQEEAERVQVGADNYVRQVLTDLSDALQRQLASINKGLDSLK"
     gene            complement(1720..3588)
                     /locus_tag="VF276_15535"
     CDS             complement(1720..3588)
                     /locus_tag="VF276_15535"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX6608312.1"
                     /translation="MSTPQATPVRPQKGFGAGAGAALAMIAVMGVLRFSFGVPSIPEL
                     LQGAFLRLVPGDLFEFMIHLLGPGAKVLLLVSILEGMVLVGGLLGWLFVRNWRPAAPG
                     AGTLARMAAERNWSGVLYGLLIGVALVVIFFLLYALGVFNPQPSTSVLLPVSISLILY
                     GLVYGLSLISLLPWTAPGAASAPKEAPAATTIAGADRRDFLRVAGGTALALVAGAGLW
                     GVLTRLLAEPTQAELVAAESGVVPTSTVNPTEAADIATAIVESGGVPRTPAAQAAPPT
                     DTPAAVAQQPAPTDTPAAVAQEPAATETAVPPTETAIPATEVPAVPTATPFPAVPILS
                     PEITPTENFYITTKNFTDPTVDASKWTLTIKGMVDNPMTLTLDQIKAMPQVKVIHTLE
                     CISNTVGGDLIGNGRWTGIRVADLMQQVRPQAGVVDVIFRAADDYSDSVPLSVLMNQD
                     TVLAYEMNGRPLQVKHGYPARLLIPGIFGMKNVKWITSMEMVSYDYKGFWQEQGWSDP
                     APYLTMSRIDYPTEGIVNQKPLYIGGIAFAGNRGIKRVEVSVDGGKTWADAQLRRPLG
                     RNTWILWTYPWVPAQAGDTTLVVRATDGTGQVQTSKDQPNYPDGATGYHRVAVKIV"
BASE COUNT          616 a         1208 c         1260 g          594 t
ORIGIN      
        1 gctgggcatc gaggcgctat cgcgcggcgc ggggtggtgc gacttcgtgg agcagaacgc
       61 gggggtgggc cgcatcatcg ccgccaacct ggccttcacc cgcctggccg agcgcgcccg
      121 cgtgcacagc atggccgtgg atgcctacct gaagctgcgc gccgccgggc gcagcctgct
      181 gccgggtgac aaagatccct tgcccgccgt taagtgtgat ataatattta tggatccgcc
      241 ctacgccgac ccggcgattc gcgagattct gggccaagtg gccgcgggac cgtttctcgc
      301 gcccgacggg ctgctggtcg tcggccacgc cacgaccctg gagctgccgg acgcgcctgg
      361 cccgttgcgc cgcatccggc accgcacctt cggcggatcg gcgttctcgc tctacaaata
      421 cgcggcggcc gccggcagcg aggccgatcc gggtgacgcg gacgccgcgc cggacgaaga
      481 cgacgacgag gagacctgtt aattgcgtat cgccatctat cccggcacct tcgatcccat
      541 caccaatggt cacctcgaca ttgcccggcg ggcgggtctc ctgttcgata agctgatcat
      601 cgccgtgggc gagaacccgc agaagaaacc gatcttcacc gtcgaggagc gcatggcgct
      661 gatccgggag gcggtgcgcg agttcgacca tagcgacatc gaagtcgaca gcttcagcgg
      721 cttgaccatc gattacgcca aggagatcgg tgcccaggcc atcgtgcgcg gcttgcgcgt
      781 gctgaccgac ttcgagtggg aactgcaact ggccctgatc aacgagaagc tggcgccgga
      841 aatcgagacg gtctgtctca tgacctcgca agaacactcc ttcttaagtg cgagcatcgt
      901 ccgcgaactg gctctgcacc acagccccaa gctgcgcgag ctggtgccgc cgcacgtcgc
      961 cgcggcgctc gacgcggtct acggggtggg cgcgccgagc acggcggggg gcagcgatcg
     1021 atctgatgtt tagccagtac ggtggctgac gaacacgggg ggtgaccgcc atcagcgaca
     1081 tgcgtatgga tttgcaatat ctgatcgatc ggctcgaaac gatggtacga gccggccgcc
     1141 gcgtgcccgg tctcaaccgg ctcatgctcg acgagcagga gctgatcgac ctgatcgacc
     1201 agatgcgtac cgtgctaccc gaggagatcc gttacgccaa gcacgtgttg cgcgaaaaag
     1261 agcagattct ttcggacgcc caggctcagg ccgacgagat cctgcggacg gcccgccagc
     1321 aggccgaggt gttgatcgag cgcgaaggct tgctcaagca ggcgcgcgcc caggccgagg
     1381 acatcaagcg cgaagcccag gaagaagccg agcgagtgca agtgggcgcc gacaattacg
     1441 tgcgccaggt cctcaccgac ctctccgacg ccctccagcg ccagcttgcc agcatcaaca
     1501 agggcctcga cagcctgaag tagcaggggg cggtccctgt cgcggtgccg cggcggtgcc
     1561 aacccggtgc cagccgtatg ccgcgccggt ggcaccgctt atggagtgcc atagcggtgc
     1621 cagggcggtg ccgccgcagg gccaggcggt ttgccccatt tccagaaaca cgaaggccgg
     1681 tccagaacgg accggccttg ctattttgcg ctctgcgggt tacacgatct tgaccgcgac
     1741 ccggtggtag ccggtcgcgc catcgggata gttgggctgg tccttgctgg tctgcacctg
     1801 gcccgtgccg tcggtggcgc ggaccaccag cgtggtatcg ccggcctggg cgggcaccca
     1861 gggataggtc cagaggatcc aggtgttgcg gcccagcggg cggcgcagtt gcgcgtcggc
     1921 ccaggtcttg ccgccgtcca cgctcacttc cacgcgcttg atgccccggt tgccggcgaa
     1981 agcgatccca ccgatgtaga gcggcttctg gttgacgatg ccctcggtcg ggtagtcgat
     2041 gcggctcatg gtcaggtagg gcgccgggtc gctccagccc tgctcctgcc agaacccctt
     2101 gtagtcgtag ctcaccatct ccatcgacgt gatccacttc acgttcttca tgccgaagat
     2161 gccggggatc agcaggcgtg ccgggtatcc gtgcttgact tgcagcggcc ggccgttcat
     2221 ctcgtaggcc aggaccgtgt cctggttcat cagcaccgag agcggcacgc tgtcgctgta
     2281 gtcgtcggcg gcgcggaaga tcacatccac taccccggcc tgcgggcgta cctgctgcat
     2341 caggtcggcc acgcggatgc cggtccagcg cccgttgccg atcaggtcgc cgcccaccgt
     2401 gttgctgatg cattcgagcg tgtggatcac cttgacctgc ggcatcgcct tgatctggtc
     2461 gagagtcagc gtcatcgggt tgtcgaccat gcccttgatg gtcagcgtcc acttcgaagc
     2521 gtcaaccgtg gggtcggtga agttcttggt ggtgatatag aagttctccg tcggcgtgat
     2581 ctcgggcgac aggatcggca ccgcagggaa cggtgtcgcc gtcggcaccg cgggcacttc
     2641 cgtggccggg atggcggttt ccgtgggcgg cacggcggtc tcggtggcgg caggctcctg
     2701 ggcgactgcg gcgggcgtat cggtgggggc gggttgctgc gccacggcgg cgggcgtatc
     2761 ggtgggcggg gccgcctgtg cggcgggtgt gcgcggcacg ccgccgctct cgacgatggc
     2821 cgtggcgatg tcggcggcct cggtaggatt gaccgtgctg gtaggcacaa ccccggactc
     2881 cgcggcgacc agttcggcct gcgtcggctc ggccagcaac cgggtcagca cgccccacag
     2941 cccggccccg gccaccaggg ccagagcggt gccacccgcc acgcgcagaa aatcgcgccg
     3001 atccgccccg gcaatggtcg tggctgcggg tgcctcttta ggggcagacg cggcgccagg
     3061 cgccgtccag ggcaacagcg agatgagcga caggccgtaa accagcccgt agaggatcaa
     3121 cgaaatgctg accggcagca gcacactcgt gctgggctgc gggttaaaca cgccgagcgc
     3181 atagagcagg aagaagatca cgacgagggc cacgccgatg agcaggccgt agagcacacc
     3241 gctccagttg cgttcggcgg ccatacgggc gagggtaccg gcacccggcg cggcgggccg
     3301 ccagttgcgc acgaacagcc agccgagcag gccgcccacc agcaccatgc cttcgaggat
     3361 cgagacgagc agcaacacct tcgcccccgg cccgagcagg tggatcatga actcgaagag
     3421 atcgccgggg accaggcgca aaaaggcgcc ttgcagcaac tccgggatgc tgggcacgcc
     3481 gaagctgaag cgcagcacgc ccatcacggc gatcatcgcc agggccgccc cggccccggc
     3541 gccgaagccc ttttgcggac ggaccggtgt ggcttgtggt gtactcatcg ttcgtcaacc
     3601 tctctaccgc gtgtcacctg cgtggcggct cagcgttgca atgggccggg cgcggcccgc
     3661 ccggcggggg aatagccg
//
LOCUS       DASUYC010000950         2595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_739632,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000950 DASUYC010000000
VERSION     DASUYC010000950.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739632"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VF276_15540"
     CDS             complement(<1..265)
                     /locus_tag="VF276_15540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608313.1"
                     /translation="MEGRIVGSNALYALESDQSRLSLQLATLIKAGAAVASPPLETGG
                     GAALAFMPHDTAGYARGALFAVADGESSVAGHPGAAPALRYLAG"
     gene            867..1709
                     /locus_tag="VF276_15545"
     CDS             867..1709
                     /locus_tag="VF276_15545"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HEX6608314.1"
                     /translation="MARSPFTLPALREPRPRRIALPGAQIVALSFVLALAGLAGGGLI
                     ALKWQERSDLIQQPITRESTRETIASTITNLENEQADLKRGLIDARAQLEKLQADSAQ
                     EKAQLGDLTAAINAERVTAGMVPLEGPGVVATFQDSTAAVIPQNEDPNNYILHDYMLR
                     DVLNTLWAGGAEAISINGERILSNTPLYCVGTTVIVNATRLSPPYEVHAIGNPDVLEA
                     GLRNSPQMLQFNERAQIYDLPISIQKVAQVQVPAYSGAVAMKYAHPGDSADEPAPTAA
                     PATP"
     gene            1719..2417
                     /locus_tag="VF276_15550"
     CDS             1719..2417
                     /locus_tag="VF276_15550"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HEX6608315.1"
                     /translation="MQNWRARLLVSAVCLVLGFLMVPQFRSQRIDRTVLAQSPTDQAS
                     YISQLYASNQRQRANLEQLQAEIARYQETANGGSSIVSLIKDLQQMRMANGEVDLTGP
                     GVTVSVTGAQDAAQVIQDLVNELRTSSAEAIAVNGVRLITRSVIAAGDNGQLQVDQQP
                     IREPYLLEAIGDPATLQNALERKGGVIALLQQDPAEKIVIKVTRRTETADWLKLPKTA
                     LDLKWRYAQTAPGN"
     gene            complement(2414..>2595)
                     /locus_tag="VF276_15555"
     CDS             complement(2414..>2595)
                     /locus_tag="VF276_15555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608316.1"
                     /translation="GERDAVVTCPQCAGTRAKRTIAAFATLRATGSTTVAEAGGGGGC
                     GCGGNCACGRAHTQA"
BASE COUNT          496 a          950 c          753 g          396 t
ORIGIN      
        1 cgccggccag atagcgcagc gccggcgcgg cgcctgggtg ccccgcgaca gaggactcgc
       61 catccgcgac cgcgaacagc gcaccgcgcg catacccggc ggtatcgtgc ggcatgaaag
      121 ccagggcggc gccccctccc gtctcaagcg ggggggatgc aacggcagct ccggccttaa
      181 tcagcgtcgc gagttgtaga ctgagccgag attggtcaga ttccaacgca tacagggcgt
      241 tggagccaac aattctgcct tccatcggcg tctcctatgc aattgtgccg ccatacccgg
      301 cgtcttagct tcagcgggaa ctgggaacct gcctcctcct ggcccagtcg tatcggtgag
      361 agatattata tcccctgttg ttgggatttg tcaactaggc agggatttgt ttaccctaac
      421 tttactggcc tgccctccgg cccgaatcgc cgctgccccg ccctgaagcc acccccggca
      481 gtcgcagcgg ggtctactct accatatatc gagcggcttg tgttgcaaat gacggccaaa
      541 ttcggaaatt gtcacagatt cgcccgcccg aaaaacaatc ctggaatccg caatgctaga
      601 tggctgcatg gcctctgcaa aatcccgctg cccgagttgc accgcgacga aacgaagaac
      661 gcgaagatca ttgatacctg ctccgttctt cgcggcctgc ccgcctgctc ggtgagccct
      721 agcgctgccg ggcaggacgc ccgattccgc aaatagccac cgggggcgat aatttggtat
      781 aatcagagca gacccaccgc aacctttcgg gcgccccggt agtctaatta atagcagcgc
      841 gaacacttag caccaggacc ctgcccatgg ctcgctcgcc ctttacattg ccggctttgc
      901 gcgaaccgcg cccgcgccgc atcgccctgc ccggcgccca gatagtggcc ctgtcgttcg
      961 tgctggccct ggccgggctg gccggcggcg gcctcatcgc gctcaagtgg caggaacgca
     1021 gcgacctcat ccagcagccg atcacccgcg aaagcacccg cgagaccatc gcttccacca
     1081 tcaccaacct ggagaacgag caggccgacc tcaagcgcgg cctgatcgac gcccgcgccc
     1141 aactggaaaa gctgcaggcc gacagcgccc aggagaaagc ccagttgggc gacctgaccg
     1201 ccgcaatcaa cgccgagcgc gtcaccgccg ggatggtgcc gctggaggga ccgggcgtcg
     1261 tcgccacctt ccaggacagc accgccgcgg tgatcccgca gaacgaggac cccaacaatt
     1321 acatcttgca cgactatatg ctgcgcgatg tgctgaacac gctctgggcc ggcggcgccg
     1381 aggctatttc gataaacggc gagcgcatcc tgtccaatac gccgctctac tgtgtcggca
     1441 cgacggtcat cgtcaacgct acccgcctgt cgccgcccta cgaggtccac gccatcggca
     1501 accccgacgt gctggaagcg ggactacgca actcgccgca gatgctccag ttcaacgagc
     1561 gcgcccagat ttacgacctg ccgatcagca tccagaaggt cgcccaagtg caggtgccgg
     1621 cctatagcgg cgccgtcgcc atgaagtatg cccatccggg cgacagcgcg gacgagccgg
     1681 cgcccaccgc ggcgccggcc acgccctaga ggaccgtcat gcaaaactgg cgcgcgcgtc
     1741 tcctcgtctc cgccgtctgc ctggtcctgg gcttcctcat ggtgccccag ttccgcagcc
     1801 agcgcatcga ccgcaccgtc ctggcccagt cgcccaccga ccaggccagc tatatcagcc
     1861 agctttacgc cagcaaccag cgccagcgcg ccaacctgga acaactccag gccgagatcg
     1921 cccgctacca ggagacagcc aacggcggct ccagcatcgt cagcctgatc aaagacctgc
     1981 agcaaatgcg catggcgaac ggcgaggtcg atctgaccgg ccccggcgtc accgtgagcg
     2041 tcaccggggc ccaggacgcc gcccaggtca tccaggacct ggtcaacgag ttgcgtacat
     2101 cctccgccga ggccatcgcc gtcaacggcg tgcgcctgat cacccgctcg gtcatcgccg
     2161 ccggcgacaa cgggcaattg caggtcgacc agcagccgat ccgcgaaccc tatctcctcg
     2221 aagccatcgg cgacccggcc acgctgcaaa acgccctgga acgcaaaggc ggggtcattg
     2281 ccctgttgca gcaagacccc gccgaaaaga tcgtgattaa agtgacgcgc cgcaccgaga
     2341 ccgccgactg gctcaagctg cccaagacgg cgctcgacct caagtggcgc tacgcccaga
     2401 ctgcgcccgg caactaagcc tgggtgtgcg cgcgcccgca ggcgcagttc ccgccgcagc
     2461 cgcagccccc gccgccgccc gcctcggcca ccgtcgtgct gcccgtggcg cgcagcgtgg
     2521 caaacgcggc aatcgtgcgc ttggcccgcg tgcccgcgca ttgcgggcag gtcacgaccg
     2581 catcccgctc cccgg
//
LOCUS       DASUYC010000951         2556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1831625, whole genome shotgun sequence.
ACCESSION   DASUYC010000951 DASUYC010000000
VERSION     DASUYC010000951.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1831625"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="VF276_15560"
     CDS             complement(<1..990)
                     /locus_tag="VF276_15560"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6608317.1"
                     /translation="MDRQPTPSVEPDGGEPPGKRLPARPHDNLPAALTPFVGRGSEYT
                     QLITRLGDSGYRLVTITGVGGVGKSRLALEVARALAQRSDAVAPFPQGVYLVPLGSLT
                     ARAPLDEALATAVAGALDLSFSGPDSPVTQLLQYLREKALLLVLDGFEHVVAGARFLT
                     AVLQDAPDLTILVTSRERLNLRGEWVVELTGLPVAAPDPPGATAETEPDGAVQLFVQT
                     AQALAPAFALTPENRPAIVRICRLVEGLPLGIELAASWIRMLTCAEIADEISQNLDFL
                     TASSQDLPRQQQGLRAVFHHSWMLLTSDEQQVLRRLAVFRGSFTREAAAAVTGA"
     gene            1263..2024
                     /locus_tag="VF276_15565"
     CDS             1263..2024
                     /locus_tag="VF276_15565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608318.1"
                     /translation="MDNNRNWAGWAALGLAALALVVALSGRFGGPRDMAGYAYAYPDA
                     RWPQSVPAAPQAPVAPAVPVVPAVPAVPVVPAVPVAPQAPKMMPPAMWKWQGGPVQHF
                     GAAGAAGRFFHFAMMGLGALLLLFLAFRLFGRRGPGGMGRVRFSQAGPGPGTPGGQPY
                     PGAPPMQSNPQYPGGPPYQGGQPYQGAPPYQGAPGAPPYQGGQPYYSGQPYYGGQPQP
                     GGQPPPGGQPYPGAPQPPPPGGPPYQSGPPPEPPR"
     gene            2026..>2556
                     /locus_tag="VF276_15570"
     CDS             2026..>2556
                     /locus_tag="VF276_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6608319.1"
                     /translation="MATKTILVVDDEPGIVTVVCDYLDRAGFRVLTAGDGPTALRLAR
                     AERPSLLVLDLMLPGMDGLDITRALRQDPATHKLPIIMLTARVEEADRLIGLELGADD
                     YITKPFSPREVVARVRAVLRRVEDTAPYTGRLQIGALSIDLERRTVRRAGVPLDLTAT
                     EFDLLAVLAREPGRSFT"
BASE COUNT          376 a          882 c          920 g          378 t
ORIGIN      
        1 ggccccggtt acggctgccg ccgcctcgcg ggtgaagctg ccgcggaaca cggccagccg
       61 ccgcaagacc tgctgctcgt cggacgtgag cagcatccac gagtggtgga agacggcgcg
      121 caacccttgc tgctggcgcg gcagatcctg gctggacgcc gtgagaaaat cgaggttctg
      181 gctgatctcg tcggcgatct cggcacaggt gagcatgcgg atccaactgg cggccagttc
      241 gatgcccagc ggcaggccct cgaccagccg gcagatgcgg acaatggcgg gccggttctc
      301 cggcgtgagg gcaaaggcgg gggccagcgc ctgggccgtt tgcacgaaca actgcaccgc
      361 gccgtcgggc tccgtctccg ccgtggctcc gggcgggtcg ggcgccgcga cgggcagccc
      421 ggtcagttcg accacccact cgccgcgcag gttcagccgt tcacgcgagg tgacgaggat
      481 ggtcaggtcg ggcgcatcct gcaagacggc cgtgaggaac cgcgccccgg cgaccacgtg
      541 ctcgaagccg tcgagcacca gcagcagcgc cttttcgcgc aggtattgca gcagttgcgt
      601 gaccggggaa tcggggccgg agaagctgag gtcgagcgcg ccggccacgg cggtcgccag
      661 cgcctcgtcg agcggggcgc gcgccgtgag gctgcccagc ggcaccagat agacgccctg
      721 cgggaagggc gccaccgcat cgctgcgttg cgccagggcg cgggccactt cgagcgccag
      781 gcggctcttg cccacgccgc ccacgccggt gatcgtcacc aggcggtagc cggaatcgcc
      841 cagccgggtg atcaactgcg tatactcgct gccgcgcccg acgaaggggg tcagcgccgc
      901 cgggaggtta tcgtggggcc gcgcgggcaa gcgcttgccc ggcggttccc cgccgtccgg
      961 ctccacggat ggggtgggtt gtcgatccac agtcactctc ctggctctga acttgcctgg
     1021 cggtgaatgt acggcgtcgt tgctctatta tagcacgatt gggccgggca gatcggggtt
     1081 tgcgtgttca tcctctcgac acttttgggc cggcgccaac acgccgggcc ggcggaaggc
     1141 tgtcacattg cccagatgtc ttgcgtagat cttcataggt acttcacatt cgcgcagtat
     1201 actgttgtat atagcaccgc gaccctgcgc gcgggtgaaa atgacaacgg aggatcgaga
     1261 ggatggacaa taatcgaaat tgggcgggct gggccgcgct gggactggcg gcattggccc
     1321 ttgtggtcgc gctgagcggg cgcttcggcg gcccgcgcga tatggccggc tatgcctatg
     1381 cgtatcccga cgcgcgttgg ccgcaaagtg tgccggcagc cccgcaggcg ccggtggccc
     1441 cggcagtccc cgtggtgccc gcggtacccg ccgtacccgt ggtaccggca gtgcccgtgg
     1501 cgccgcaagc gccgaagatg atgccgcccg cgatgtggaa gtggcaaggt gggccggtgc
     1561 agcacttcgg agccgcggga gccgcggggc gattcttcca cttcgccatg atgggcctgg
     1621 gcgcgttgct gctgctgttc ctggcgttcc gtctgttcgg gcggcgcgga cccggtggca
     1681 tgggccgggt ccgcttcagc caggccggac cgggtcccgg cacaccgggc gggcagccct
     1741 atccgggcgc gccgccgatg cagagcaacc cgcaatatcc cggcgggccg ccgtaccagg
     1801 gcgggcaacc ctaccagggc gcgccgccgt atcaaggcgc gccgggtgcg ccaccatacc
     1861 agggtggaca gccctactac agcgggcaac cctactacgg cgggcagccg cagccgggtg
     1921 ggcagcctcc gccaggtgga cagccgtatc caggggcgcc gcaacccccg ccgccgggcg
     1981 ggccgccgta ccagagcgga ccgccgccgg agccgccgcg ttaagatggc gaccaagact
     2041 atcctggtgg tcgacgacga gccgggcatc gtcaccgtcg tctgcgatta cctggaccgg
     2101 gccggattcc gcgtactcac cgcgggagac ggcccgaccg ccttgcgcct ggcccgcgcc
     2161 gagcgcccca gcctgctggt gctcgacctg atgctgccgg gcatggacgg cctcgacatc
     2221 acccgcgccc tgcgccagga cccggccacg cataagctgc cgatcatcat gctcacggcg
     2281 cgcgtcgagg aggccgaccg cctgatcggc ctggagctgg gcgccgacga ctacatcacc
     2341 aagccgttca gcccgcgcga agtcgtggcc cgcgtgcgcg ctgtgttgcg gcgggtcgag
     2401 gataccgccc cgtacaccgg gcggctgcaa atcggcgcgc tgagcatcga cctggagcgg
     2461 cgcaccgtgc gccgggccgg ggtgccgctc gacctgacgg ccaccgagtt cgacttgctg
     2521 gccgtgctgg cccgcgagcc gggccggtct ttcacc
//
LOCUS       DASUYC010000952         2392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1774754, whole genome shotgun sequence.
ACCESSION   DASUYC010000952 DASUYC010000000
VERSION     DASUYC010000952.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1774754"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1424)
                     /locus_tag="VF276_15575"
     CDS             complement(<1..1424)
                     /locus_tag="VF276_15575"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6608320.1"
                     /translation="MALLDLEERRLKDLIRPERIWQVLVDGLPTDFPPLRTLDARPNN
                     LPAQPNVFIGREAQIAAVIALLRRPDVRLLTLIGPGGTGKTRLALQVAAELLDEFADG
                     VWFVDLAPLTDPALVAGSIAGVFSLQESAARSAQEVLHDYLSGKTLLLVLDNFEQVRP
                     AASLVDTLLRRAPHLEVLITSRVPLHLYGEQEHTVPPLALPATLPLPPLERLTQYEAV
                     RLFVERARTVREDFQVTNETAPAVAEICARLDGLPLAIELAAARVRLFAPEALLTRLG
                     ARLATLTGGPRNLPARQQTLRATIEWSYNLLSPGEQQLFRRLAVFQGGRTLEAIDAIC
                     NGDGTLAVDVLDGVEALIGHSLLGTRPAQDGELRFVMLETIHEYAREILQKCGEAATL
                     RQAHAQYFLRLIEQAERELLGPQHLAWMNRLEDEHDNIRAALRWALDTGAVGVALRLA
                     GALWLFWWTHVHYREGRQWLEAVLA"
     gene            complement(1382..1900)
                     /locus_tag="VF276_15580"
     CDS             complement(1382..1900)
                     /locus_tag="VF276_15580"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6608321.1"
                     /translation="MTSVQSVNANIAGGARPTGTITFLFTDVQGSTQLWERDPTWMEQ
                     AHRRHEAILHEAIAAHGGWAYKQIGDSFQAAFQTASAALAAAIAAQQALAAEPWGGPG
                     PIRVRMGLHTGTVEERADGYFGPLLNRVHRLMSAGHGGQILLTATTYELVRDHLPGGW
                     RCLTWRSAGSRT"
     gene            2194..>2392
                     /locus_tag="VF276_15585"
     CDS             2194..>2392
                     /locus_tag="VF276_15585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608322.1"
                     /translation="MYQQFIIENRADQYQQDCWRAAASERGLRAGRHQVATETAHPLP
                     HRPALAWLTFAVRRVLIVAPAG"
BASE COUNT          410 a          777 c          813 g          392 t
ORIGIN      
        1 gctagtactg cttccaacca ctgccgcccc tcgcggtaat gcacgtgggt ccaccagaac
       61 aaccagaggg caccggccag gcgcagcgct acccccaccg cgccggtgtc cagcgcccag
      121 cgcagggccg cccggatatt atcgtgttcg tcctccaggc ggttcatcca ggccaggtgc
      181 tgcggtccca gcaactcgcg ttccgcctgc tcgatcaggc gcagaaagta ttgcgcgtgg
      241 gcctggcgca aggttgccgc ttccccacac ttctgcaaga tttcccgcgc gtactcgtgg
      301 atggtttcca acattacgaa gcgcagctca ccgtcttgcg ccggccgcgt cccgagcaag
      361 ctgtggccga tcagcgcttc gacgccatcc aggacatcga cggccagggt gccgtcgccg
      421 ttgcagatcg catcaatggc ttccagtgtg cggccgccct ggaagacggc caggcggcgg
      481 aacaactgct gttcgccggg actgagcagg ttgtagctcc actcgatggt ggcccgcagg
      541 gtctgctggc gcgccggcag gttgcgcggg ccgcccgtca gcgtcgcgag gcgggccccc
      601 aggcgcgtca gcagtgcctc cggcgcgaac aaccgcaccc gtgccgcggc tagctctatt
      661 gccagcggca gcccgtcgag tcgcgcgcag atctcggcca cggccggggc cgtctcatta
      721 gtgacctgga agtcctcgcg cactgtgcgt gcgcgctcca caaagaggcg caccgcctca
      781 tattgggtga gccgctccag cgggggcaag ggtagggtgg cggggagcgc caacggcggc
      841 acggtatgct cctgttcgcc atagagatgc agtggcaccc ggctggtgat caacacctcg
      901 aggtggggcg cccggcgcag gagggtatcg acgagcgacg ccgcaggccg gacctgctcg
      961 aagttgtcga gcaccagcaa caaggttttg ccgctcagat agtcatgcaa cacctcctgc
     1021 gcactccgag cggcactttc ctgcagactg aagaccccgg cgatgctgcc cgcgaccagg
     1081 gccgggtcgg tgagcggcgc caggtcgacg aaccagacgc cgtcggcgaa ctcgtcgagt
     1141 agttcggcag cgacttgcaa ggccaggcgg gtcttgccag tgccgccggg tccgatcagc
     1201 gtgagcaggc gcacgtcggg ccgccgcagc agggcaatca cggcggcgat ctgggcttcg
     1261 cggccgatga agacgttggg ctgggcgggc aggttgttgg gccgggcatc gagtgtccgc
     1321 aggggtggaa agtcggtggg caggccatcc acgagcacct gccagatgcg ttcggggcgg
     1381 atcaggtcct tgagccggcg ctcctccagg tcaagcagcg ccaccccccc ggcaggtgat
     1441 cccgcacgag ctcgtaggtg gtggcggtga gcaagatctg gccgccgtgg ccggcactca
     1501 tgaggcgatg gacccggttg agcaggggac cgaagtagcc gtcggcccgc tcctcgaccg
     1561 tgcccgtatg caggcccatg cgcacgcgga tcggccccgg gccgccccag ggctcggcgg
     1621 ccagggcctg ttgggcggcg atagcggcgg cgagggcggc ggaggcggtc tggaaggcgg
     1681 cctggaagga atcgccgatc tgcttgtagg cccagccccc gtgcgccgcg atcgcttcat
     1741 gcaggatagc ttcgtggcga cgatgggcct gctccatcca cgtgggatcg cgctcccaga
     1801 gttgcgtgct gccctgcacg tcggtaaaca ggaacgtgat cgtgcccgtg ggccgcgccc
     1861 cgccggcgat gttcgcattt acgctttgga cggatgtcac tggccagtct cccacattcc
     1921 agcggtcgag tgtattagca gcatctttat cataccagaa aaaaccatgg accacgctat
     1981 ccctgattgc tactttcgtg gccgattgtg ctgtaagtac cacatgcgct aggagtgcgg
     2041 ataactcatt cgagtgagcg gaggctgacg catccatact caatcaagtg atgtgtggga
     2101 tcggccgccg gcctatactg atggcaagtc ggcaacgacc acaaccggaa cttccataag
     2161 aaagagatgc taaccgccga aaggagcgcg acgatgtacc agcaatttat cattgagaac
     2221 cgggcggacc agtatcagca ggactgctgg cgggcggcgg cctcggagcg cgggttgcga
     2281 gccggtcgcc accaggtagc cacggagacg gcgcaccctc tgccgcaccg gccggcgctc
     2341 gcatggctca cctttgcggt gcgccgcgtc ctgatcgtgg ccccggcagg ta
//
LOCUS       DASUYC010000953         2143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1355788, whole genome shotgun sequence.
ACCESSION   DASUYC010000953 DASUYC010000000
VERSION     DASUYC010000953.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1355788"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1447)
                     /locus_tag="VF276_15590"
     CDS             complement(<1..1447)
                     /locus_tag="VF276_15590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608323.1"
                     /translation="MIAVRRLLIVAAYLVLILAFTWPLPAALGDHLVLARGSDVWPHI
                     WNFAWVRTALVDLHHSPYFTHSIFYPTGVPLVYHALNIFTATVSIPLQALFGLVAAFN
                     LMLLANLLAAALAAYWLGRVLGLDRLPAFLLGLLFACSPPVSAAVSQGQAELASVFWM
                     PLYAGLLVRGGGLRLRGLPPGGWPYLAGAALALVGSALAIWYWFVSLLVFTAVYGLVE
                     LWAVWRTGDRRAMAGVVGRLALVGGVALVLLSPLLVLMVQEQLRSGADVGIARSGTSD
                     AVVLNGSQDPLAWFRPLPGHIDIAQEFLNGANLGLGWTVLALIVVAVVLLRGPRRRGL
                     VLWGLAALALAVLALGPHLVLNGESTGIPLPYSAILQLPGAAAMRVPLRFATLLALCL
                     AVPAAFGLAAILDRVRPAAGQLAVTALAVALVVVEFFGPPRVLIQPEAQPFFQQLAAH
                     PSPCLGDLREPLAQCDAVLELPQATWIPPGLY"
     gene            complement(1444..1647)
                     /locus_tag="VF276_15595"
     CDS             complement(1444..1647)
                     /locus_tag="VF276_15595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608324.1"
                     /translation="MRWALVIGLWVVLLALLALAVRNFGWLTTWASGLFLSCLSVCGV
                     IIGFSLIAAAITLSVDTIKGRYP"
     gene            complement(1682..1846)
                     /locus_tag="VF276_15600"
     CDS             complement(1682..1846)
                     /locus_tag="VF276_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015812275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HEX6608325.1"
                     /translation="MRSRLSVLGGLFGFMVQGSRKYWLLPLVIALMVVVLAIVIGGSS
                     VLAPFIYPLF"
     gene            complement(1895..>2143)
                     /locus_tag="VF276_15605"
     CDS             complement(1895..>2143)
                     /locus_tag="VF276_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015165375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX6608326.1"
                     /translation="GLYYQVIREFFGHTGVPVIVNTSFNVRGEPIVNTPLEAYNCFSH
                     TDMDYLVLGNCLVDKTAKKIIAAYAGHKGPRVKEEVIV"
BASE COUNT          414 a          736 c          744 g          249 t
ORIGIN      
        1 ggtacaggcc gggtgggatc caggtggcct gcggcagttc gagcacggcg tcgcattggg
       61 ccaatggttc gcgcagatcg cccaggcagg gcgaggggtg cgcggccaac tgctggaaga
      121 agggctgggc ttcgggctgg atcagcacgc gcggcgggcc gaagaactcg acgaccacca
      181 gcgccacggc cagcgcggtg acggccagtt gccccgccgc cgggcgcacg cggtcgagga
      241 tcgccgccag gccgaacgct gccgggacgg ccaggcagag cgccagcagc gtggcgaagc
      301 gcagcggcac gcgcatggct gccgcgccgg gcagttgcag gatggccgag tagggcagcg
      361 ggatgccggt cgattcgccg ttcagcacca ggtgcgggcc gagcgccagc acggccagcg
      421 ccagcgcggc cagcccccag agcaccagcc cgcgccgccg cggcccgcgc agcagcacca
      481 cggcaaccac aatcagggcc agcaccgtcc agcccaggcc caggttggcg ccgttgagga
      541 actcctgggc gatgtcgatg tggccgggca gcgggcggaa ccaggccagc gggtcttgcg
      601 agccgttgag caccacggca tccgaggtgc ccgagcgggc gatgcccacg tcggcgccgc
      661 tgcgcaactg ttcctgcacc atcagtacca gcagcgggct gagcagcacc agggcgaccc
      721 cgccgaccag cgccagccgc cccaccacgc cggccatcgc ccgccggtcg ccggtgcgcc
      781 agaccgccca cagctcgacc agcccataga cggcggtgaa gacgagcagg ctgacgaacc
      841 agtaccagat cgccagggcc gacccgacca gggccagcgc cgctccggcc aggtacggcc
      901 acccgcccgg cggcagcccg cgcagccgca gcccgccgcc gcgcacaagc aggcccgcgt
      961 acagcggcat ccagaagacg ctcgcgagtt cggcctgccc ctggctgacg gcggcgctga
     1021 caggcgggct acaggcgaac agcagcccca gcaggaaagc gggcaggcgg tcgaggccga
     1081 gcacgcgccc gagccagtag gcggccaggg cggcggccag caggttcgcc agcaacatca
     1141 ggttgaacgc cgccaccagc ccgaacagcg cctgcaacgg gatcgagacc gtggcggtga
     1201 agatgttgag ggcgtggtag accagcggca cgcccgtggg gtagaagatg ctgtgggtga
     1261 agtagggcga gtggtgcagg tctaccaggg ccgtgcgcac ccaggcgaag ttccagatat
     1321 gcggccagac atcgctgccc cgcgccagca caagatgatc gccaagggcc gcgggcagcg
     1381 gccaggtgaa ggcgaggatc aggacgaggt aggcggcgac tatcaacagc cgccggaccg
     1441 cgatcaaggg tagcggccct tgatcgtgtc cacggagagc gtgatcgcgg cggcgatcag
     1501 cgagaagccg atgatcaccc cgcacacgct gaggcagctc aagaacagcc ccgacgccca
     1561 ggtggtcagc cagccgaagt tgcgcacggc cagcgccagc agcgcgagca agaccaccca
     1621 cagcccgatg acgagcgccc agcgcacacc cggcgataga gtccttggtg tattcatcgg
     1681 gctagaacag cgggtagatg aacggggcca gcacgctgct gccgccgatc acgattgcca
     1741 ggaccacgac catcagcgcg atgaccagcg gcaacagcca gtatttccgg ctgccctgca
     1801 ccataaaccc gaatagaccg cccagtacgg acaggcgaga tcgcattttc ccctccaaac
     1861 cgccgctgga gacgagccgg cggctgcgcg tgtgctaaac gataacttct tctttgaccc
     1921 gcggtccctt gtggcccgcg taggcggcaa tgatcttctt cgccgttttg tcgaccaggc
     1981 agttgcccag caccaggtaa tccatgtcgg tgtggctgaa gcagttgtac gcttccagcg
     2041 gcgtgttgac aatcggttcg ccgcgcacgt tgaacgacgt gttcacgatc accggcaccc
     2101 cggtgtggcc gaagaactcg cggatcacct ggtaatagag gcc
//
LOCUS       DASUYC010000954         3554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1774755, whole genome shotgun sequence.
ACCESSION   DASUYC010000954 DASUYC010000000
VERSION     DASUYC010000954.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1774755"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="VF276_15610"
     CDS             <1..833
                     /locus_tag="VF276_15610"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX6608327.1"
                     /translation="RYVAEQDETGAPIVAPTPSFQARVIYPMFDQVGRRLNALTPTSY
                     LDQIQKLLYQVGPPYRITRMGFLGMQVGAGLGLMLLLLLWAVVNAPRAPGTWLLAALL
                     GLFAGRYLPYFFLVRRATQRKKLLLRSLPAALDFLAIMVEAGMGFDAALNELVRRWQN
                     TVTDEFALLLIDFQIGKPRRDAWRDLTVRTQLAELNSFVVAMMQSEQTGASIRSLLRT
                     QAEQMRIRRRQRAEEEARIAPVKMLVPMGLFIFPCILIVILGPAMPQILTTFKNLAGH
                     "
     gene            1033..3261
                     /locus_tag="VF276_15615"
     CDS             1033..3261
                     /locus_tag="VF276_15615"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEX6608328.1"
                     /translation="MRQTEEERGGEDASRWNRIDTALCAALALAALAVRVPELLTLPA
                     FRDELAEVVRGLHIYRGLSYPLTNEALDIGAFFNYLVALLFAGAGPDPYTPRLLVTMF
                     GAATVALTYALARVLTGARLPALLAGAFLATNSMHILVGHVAWSNCMTPFWTTATLLA
                     LAPGRGRTPDGLPGPRALLPAGLCAGLALQTHSSVIVFLVGVGAWLWWPGPALRLRLR
                     RPAFWGFHAMFLLAYANMIVFNIVNPLASWYHVVEHKGYALAGAAPGSETVAVGAGYP
                     DNLLALLVEWVRANSADTVRYDSVVRYLADPQMIVYGALLVAGVVLAARRRYWLILAP
                     LVSGVLIMPLFNRDYQFEIHTRYIGFLLPLGAILTALPIAAAVARIRAGQWRLRPQAA
                     WAGAALAAVLVLYPVPALANYYAEETAAGRSNSGVIATLDALATMRGGGAVAYTSKLP
                     QAMSIGLGLDVWEIPHVELINDNDPALPPGAGGLDTPAVWRAFVDWARQLPPGTREFT
                     VALPPAAVDSWQAIGGDLPWGAPVAVRGSDGAPAATLLRLPLSAAGLAAAPAARLQTD
                     LLVIVPRLADALAPATRDRLTSALAAGRSVRVLALIGAEDPGFTGARAALTALGLPDD
                     HVLLGGYPAAFLRRLAPDSGVADPEAPWTDPATGATQAGTASAPTVHARFFGAPAPLQ
                     AAALRADLRAAAVLYTPGAILAPPGDNPDPVALVTERTAAYAARKAGVPLEILPASAA
                     GR"
BASE COUNT          460 a         1309 c         1209 g          576 t
ORIGIN      
        1 atcgctacgt ggccgagcag gacgagaccg gcgcccccat tgtggcgccg acgcccagct
       61 tccaggcccg cgtgatctac ccgatgtttg accaggtggg ccgccggctc aatgcgctca
      121 cccccaccag ctatctcgat cagatacaga agctgctgta ccaggtcggc ccgccctacc
      181 gcatcacgcg catgggcttc ctgggcatgc aggtcggcgc ggggctggga ctgatgctgc
      241 tgctcttgct ctgggcggtc gtgaatgcgc cgcgggcgcc cggcacctgg ctgctggccg
      301 cgctgctggg cctgttcgcc ggccggtacc tgccctattt cttcctggtc cgccgggcca
      361 cgcagcgcaa gaagctgctg ttgcgctcgc tgcccgccgc gctcgacttc ctggcgatca
      421 tggtcgaagc gggcatgggc ttcgacgccg ccttgaatga gttggtgcgc cgctggcaaa
      481 ataccgtcac ggacgaattt gcgctgctgc taatcgattt ccagatcggc aagccgcggc
      541 gcgacgcctg gcgcgacctg accgtgcgca cgcagcttgc ggaactgaac tccttcgtgg
      601 tcgccatgat gcaaagcgag cagacgggcg ccagcatccg cagcctgctg cgcacccagg
      661 ccgagcagat gcgcatccgc cggcgccagc gcgccgagga agaagcgcgc atcgcgccgg
      721 tcaagatgct ggtgccgatg ggcctcttca tcttcccctg catcctgatc gtcatcctcg
      781 gcccggccat gccgcagatt ctcaccacct tcaagaacct ggccggtcac taaacggcgc
      841 accgccgcag tggggccggg cgcgcaggcg cccggccctt ttgcgtggac cgcgcggagg
      901 ccccgtcccg ccgcgtttcc tgctgattcc gcgattttcc catcaaaacg ggcccgagta
      961 tgctataata ttgggttcac cgacacccac ggaaaggggt acgcgtgtta cctgatgggc
     1021 gcgcaaagat tcatgcgaca aacagaggag gaacgcggcg gcgaagatgc aagccgctgg
     1081 aaccggatcg acacggcgct atgcgcggca ctggcgctgg ccgccctggc ggtgcgcgtg
     1141 cccgaattgc tgacgctgcc cgccttccgc gatgagctgg ccgaggtggt gcgcgggctg
     1201 cacatctacc gcggcctgag ctatccgctg accaacgagg cgctggacat cggcgcgttc
     1261 ttcaactacc tggtggcgct gctgttcgcc ggcgccggcc ccgaccccta caccccgcgg
     1321 ctgctggtga cgatgttcgg ggccgccacg gtggccctga cctacgcgct ggcccgcgtg
     1381 ctcaccgggg cgcggctgcc cgccctgctg gcgggggcgt tcctggcgac caactccatg
     1441 catatcctgg tggggcacgt cgcctggtcg aactgcatga cgccgttctg gaccaccgcc
     1501 accctgctgg ccctggcgcc gggccgcggg cggacgcccg acggcctgcc cggtccgcgc
     1561 gcgctgctgc cggccgggct ctgcgccggg ctggccttgc agacccactc gtctgtgatc
     1621 gtcttcctgg tgggcgtagg cgcctggctg tggtggccgg ggccggcctt gcgcctgcgg
     1681 ctgcgccgcc cggccttctg gggcttccac gcgatgttcc tgctggctta tgccaacatg
     1741 atcgtcttca atatcgtcaa tccgctcgcc tcgtggtatc acgtcgtcga gcacaagggc
     1801 tacgccctgg cgggcgccgc gcccggctcg gagacggtag cggtgggcgc gggctatccc
     1861 gacaacctgc tggccctgct ggtcgagtgg gtgcgcgcca acagcgccga caccgtgcgc
     1921 tacgacagcg tcgttcgcta cctggccgac ccgcaaatga tcgtctacgg cgcgctgctg
     1981 gtggccgggg tggtcctcgc cgcgcggcgc cggtactggc tcattctggc gcccctcgtg
     2041 agcggcgtct tgatcatgcc cctgttcaac cgcgactacc agttcgagat ccacacgcgc
     2101 tacatcggtt ttttattgcc gctaggcgcc atcctgacgg ccctgcccat tgccgcagcc
     2161 gtcgcgcgca tccgcgccgg gcaatggcgc ctgcgccccc aggccgcgtg ggcgggggcg
     2221 gccctggccg cggtgctggt gctctacccg gtgccggcgc tggcgaacta ctacgccgag
     2281 gagacggccg cggggcgcag caacagcggc gtcatcgcca ccctggacgc gctggcgacg
     2341 atgcgcggcg ggggcgcggt ggcctacacc agcaagctac cgcaggctat gtccatcggg
     2401 ctgggcctcg acgtgtggga gatcccgcat gtggaactga tcaacgataa cgatcccgcc
     2461 ctgccgccgg gcgccggggg gctcgacacc ccggcggtct ggcgtgcctt cgtggactgg
     2521 gcgcggcagt tgccgccggg cacacgtgag tttaccgtcg ccctgccgcc cgccgcggtc
     2581 gattcctggc aggctatcgg cggcgatctg ccctggggcg cgccggtcgc ggtccgcggc
     2641 agcgacgggg cccctgccgc caccttgctg cgcttgccgc tgagcgccgc gggcctcgcc
     2701 gccgccccgg cggcccggtt gcaaaccgac ctgctggtga tcgtgccccg gctcgccgac
     2761 gccctggccc cggccacgcg cgaccggctg acgagcgcgc tcgccgcggg ccggagcgtg
     2821 cgcgtgctgg ccctcatcgg cgccgaggat cccggcttca ccggcgcgcg cgccgccctg
     2881 accgcgctcg gcctgccgga tgaccacgtg ctgttgggcg gctacccggc ggccttcttg
     2941 cgccggctgg cccccgattc cggcgtagcc gatccggagg cgccctggac cgatcccgcc
     3001 accggcgcca cccaggcggg caccgccagc gcgcccaccg tccacgcccg cttcttcggg
     3061 gcgccggccc cgctgcaggc ggcggccctg cgcgccgatc tgcgcgccgc cgccgtgctc
     3121 tatacccccg gcgccatcct ggccccgcct ggcgataacc ccgacccggt cgcgctcgtc
     3181 accgagcgca ccgccgccta cgccgcccgc aaagccggcg tgccgctgga gatcctcccg
     3241 gccagcgccg ccgggcggta ggtgccggtt cccgttctcc ctgtcatgct gaatagcctc
     3301 tcacagcttc cctgtcattc tgagcggagc gaagaatctc cgtctcgtgc gggttggcga
     3361 cgagactctt cgctgcgctc agagtgacaa ggattcagag tgacacggat ggggagagct
     3421 acccggccag gtagacgctg ccgtccggcg tcaccgtgag gttgatgggg gccagcgggc
     3481 ggcgggccgg gccacggacg accgcgccgt tcgatgaggg atcgaacagg gcgccatggc
     3541 acgggcagcc cagc
//
LOCUS       DASUYC010000955         2722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_852959,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000955 DASUYC010000000
VERSION     DASUYC010000955.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852959"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1227)
                     /locus_tag="VF276_15620"
     CDS             complement(<1..1227)
                     /locus_tag="VF276_15620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608329.1"
                     /translation="MTSHSARAWRAGWLGLLALLLVGTALAPLPPAAAQTGPRGTIPH
                     TAVNPYGANVFLHKEAEQEKVTRTLDLAAEAHLAWIKQDFQWSDIEISKKGDFTDTRN
                     GPAKSAWDKYDFIVDQAAQRNIQVVARISFAPDWARNPGSGANDAPRSNDDLADFIVA
                     LLNHYQGRVKYIQIWNEPNLAYEWKAGGKVDPAGYVEMLKTVYPKAKAADPNVSILSA
                     PMAITLEQIDYAGNLNELDYWDQMYKAGAKDYFDVLSANAYGLDLPPDDPPAPDKLNF
                     RRVELLHEVMVKNGDADKAVWFNEYGWNATPTTLPESEQLHWRRVDPDTQAKWTVDGI
                     QYALDNWPWAGVFFIWYFRQVGDIPDTKAEYYFRMVDPDFTVQPVYKAIRRAAGLYQG
                     PGALVPTPTAGAAATAT"
     gene            complement(1439..>2722)
                     /locus_tag="VF276_15625"
     CDS             complement(1439..>2722)
                     /locus_tag="VF276_15625"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEX6608330.1"
                     /translation="SADGGFVAFDSDATNLVPGDTNGVYDVFLHDLQTGATERASLTA
                     TGGEPDGDSSTPSLSADGRFVAFQSYAGNLVAGDANGNYDVFVRDRQTGAVERSSLGV
                     GGAEGNAGAYEPSLSADGQIVAFKSGSSNLVPGDGNGSADVFVRDRTTGALDRASLAT
                     DGAEGDADSGRPATSATGRYVAFESAAGNLVAGDSNGVFDVFLRDRGEVPPPSPTPTA
                     SPTVAPTATPPATATVTPCPVTFSDVPPAQPFYPFIRCLACQGIVGGYSDGTFLPGAN
                     VTRGQVAKFVSNAAGYTDAVPPTRQTFRDVAPDSTFWLFIERAVAHGVISGYDCGPGC
                     LEFRPGAAVTRGQVAKFVANAAGFADAIPPAQQTFADVPPDSTFWLFIERTAAHGVVG
                     GYSDGTFRPGAPVTRGQTSKFIANAFFPGCNPAAR"
BASE COUNT          400 a          891 c          989 g          442 t
ORIGIN      
        1 agtcgccgtc gcggcggcgc ccgccgtggg cgtgggcacc agggcgcccg gcccctgata
       61 gagcccggcg gcgcggcgga tggccttgta gaccggttgc accgtgaagt ccgggtccac
      121 catgcggaag tagtattcgg ccttggtgtc ggggatgtcg cccacctggc ggaaatacca
      181 gatgaagaac acgcccgccc acggccagtt gtccagcgcg tactggatgc cgtccaccgt
      241 ccacttggcc tgcgtatcgg ggtccacccg ccgccagtgc aactgctccg actcgggcaa
      301 cgtggtcggc gtggcgttcc agccgtactc gttgaaccag acggccttgt cggcgtcgcc
      361 gttcttgacc atgacctcgt gcagcagttc cacgcggcgg aagttcagct tgtcgggcgc
      421 gggcgggtca tccggcggca ggtccaggcc ataggcgttg gcggagagga catcgaagta
      481 gtccttggcc cccgccttgt acatctggtc ccagtagtcg agttcgttca ggttgccggc
      541 gtagtcgatc tgctccagcg tgatcgccat cggcgcactc aggatcgaga cgttgggatc
      601 ggcggccttg gccttcggat acaccgtctt gagcatctcg acatagcccg ccgggtcgac
      661 cttgccgccg gccttccact cgtaagccag gttcggctcg ttccagatct ggatatactt
      721 gacccggccc tggtagtggt tcagcaaggc cacgatgaag tcggccaggt cgtcgttgct
      781 gcggggcgcg tcgttggcgc cgctgccggg gttgcgcgcc cagtcggggg cgaacgagat
      841 gcgcgccacc acctggatat tgcgctgcgc cgcctggtcc acgataaagt cgtacttgtc
      901 ccaggcgctc ttagccgggc cgttgcgggt gtcggtgaag tcgcccttct tggaaatctc
      961 gatatcgctc cactggaagt cctgcttgat ccaggccagg tgggcctccg cggccaggtc
     1021 cagcgtgcgg gtcaccttct cctgctcggc ttccttatgc aagaagacgt tagcgccgta
     1081 gggattgacg gcggtgtggg gaatggtgcc gcgcgggccg gtctgggcgg cggcgggcgg
     1141 cagcggcgcg agcgcggtgc cgaccagcag cagggccagc aggcccagcc aaccggcccg
     1201 ccacgcgcgg gcagagtgcg aagtcaaaac gaagatcctc ctcagtctag tgtcccgccc
     1261 ctgggcgcgc ccgtgtgccg ggcgctcagt tatccggggc atcgaaagaa tacccgatcc
     1321 ggcgccgctg ccgcaaggtg acggcaggcg taagctgcat caatgggcac tccgggcggc
     1381 gcgcggggtg tgtatgctgt gcgtcagggc gcgccacccg gaatgatcat tgaacgaact
     1441 agcgcgccgc cgggttgcag ccggggaaga aggcgttggc gatgaacttg ctcgtctgcc
     1501 cgcgcgtaac cggggcgccg gggcggaatg tgccgtcgct gtagccgccc accacgccat
     1561 gcgccgccgt gcgctcgatg aacagccaga aggtgctgtc gggcggcaca tcggcgaacg
     1621 tctgctgggc gggcgggatc gcgtcggcga agcccgcggc gttggcgacg aacttggcga
     1681 cctggccgcg cgtgacggcg gcgccggggc ggaactccag gcaaccgggg ccgcaatcgt
     1741 agccgctgat cacgccatgg gccaccgcgc gctcgataaa cagccagaag gtgctgtcgg
     1801 gcgccacgtc gcggaaggtc tggcgggtgg gcgggacggc gtcggtgtag cccgccgcgt
     1861 tgctgacgaa cttggcgacc tggccgcgcg tcacgttggc acccggcagg aaggtgccgt
     1921 cgctatagcc gccgacgatg ccctggcagg ccaggcagcg gatgaacggg tagaacggct
     1981 gggcgggtgg cacatcggag aatgtcaccg ggcagggggt gacggtcgcc gtggcgggcg
     2041 gcgtcgccgt cggtgccacc gtgggcgaag cggtgggcgt ggggctgggc ggtgggacct
     2101 cgccccggtc gcgcaggaag acatcgaaca cgccgttgct gtcgcccgcc accaggttgc
     2161 cggcggcgga ctcgaatgcc acgtagcggc ccgtcgcgct ggtcgccggg cgcccgctgt
     2221 cggcgtcgcc ctcggcaccg tccgttgcca gcgacgcccg atccagagcg ccggtggtcc
     2281 ggtcgcggac aaagacatcg gcggacccgt tgccgtcgcc gggcaccagg ttgctcgacc
     2341 cggacttgaa ggcgacgatc tgcccatcgg cgctcagact cggctcgtag gcgcccgcat
     2401 tgccctcggc gccgcccacg cccaggctgc tgcgctccac ggccccggtc tgccggtcgc
     2461 gcacaaacac gtcatagttg ccgttggcgt cgcccgcgac caggttgccg gcgtaggact
     2521 gaaaggcgac gaagcgcccg tcggcgctca ggctcggggt agaactgtcg ccatcaggct
     2581 cgccccccgt ggcggtcagg ctggcgcgct cggtggcgcc ggtctggagg tcatgcagga
     2641 acacgtcgta gacgccgttg gtgtcgccgg gcaccaggtt ggtcgcgtcg ctgtcgaagg
     2701 cgacaaagcc accatcggcg ct
//
LOCUS       DASUYC010000956         4701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_853176,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000956 DASUYC010000000
VERSION     DASUYC010000956.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853176"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="VF276_15630"
     CDS             complement(<1..575)
                     /locus_tag="VF276_15630"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HEX6608331.1"
                     /translation="MTRVAPAARPSAIRRFISQHPLASLFVLIYALEWLTMVPTALES
                     QGLLPFHMPDILMFTTGWAPAIAAVIVTRAVSGKTGVRALFSRFLRWRVGIQWYVVAL
                     GTIAALILAGLGLYGALGNPVPALPIGTVSLGTVVFAFVANMGLGLLLNTEEIAWRGV
                     ALPRLQATHSALVASLLLAVVEGLTHLPYAWT"
     gene            984..2525
                     /locus_tag="VF276_15635"
     CDS             984..2525
                     /locus_tag="VF276_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX6608332.1"
                     /translation="MGLAKVRSCAIRGLDGSLVEVEVDVSHGLPGFFIVGLPDTTVQE
                     SKERVRSAVRNSGGVFPAKRVVVSLAPADVKKESSAYDLPIAVGVLAATGQVTADLDD
                     TVLLGELGLDGVLRHTTGILPMVALAREREIRQVYVPEDDANEAALVAGITVYPVRTL
                     AQLVAHLNSVGAHGSNGIAPVPYRDHLFTVEDAGAGHVDLQEVKGQEHVKRALEVAAA
                     GSHNLLMQGPPGSGKTLLARCLPSILPAMTRDEALEVTKIYSVAGMLPAGQPLIRTRP
                     FRAPHHTASHAALVGGGRQPRPGEISLSHRGVLFLDELLEFDQPTLENLRQPLEDRVI
                     TITRVSGTLTFPASLILIAACNPCPCGYFGDSQKACVCSPSTVTRYKKRLSGPLLDRI
                     DIHVEVPRVAYEKLAGTTLGESSAAVRQRVEAARAIQRARFAGLPILTNSEMRPAEIR
                     QFCVLDSAGQALIKAAMRQLNLSARGYHRVLKLARTIADLAGAQGIGAVHLAEALQYR
                     AREGV"
     gene            2710..>4701
                     /locus_tag="VF276_15640"
     CDS             2710..>4701
                     /locus_tag="VF276_15640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608333.1"
                     /translation="MLKRVGGSRIFRAGVALALLLLVALVGAQLSHAPAAPAWGGARP
                     AGLAGGPAAPGAAGDVAEAPVTFGDNGFVFGRSVKNDVSPPLRSIKPAPVQPATTIRE
                     MPEPAGEDMSKAAPLAPVTDPVVQRAFGPDVLAPLAMPAPIQNFDGVNNIDGVYPPDT
                     NGDVGPNHYVQWVNLHFQIWDKNGNSLYGPAAGNTLWSGFGGACQSQNAGDPVVLYDP
                     LADRWVLSQFTSSSPYGECVAVSTTGNPTGSYYRYFFQFSTSVFYDYPHMGVWPDGYY
                     MSANRFGGVFQSYQGASAIVFDRAKMLNGQPATYQEKQTSSSYGTLLPSDLDGTTPPP
                     AGEPNFFAAKGSTTLDLWKFHVDWLTPGNSSLTGPTRLGVAAYNLLCASTRNCVKQPS
                     TSVGLDGLGDRLMHRLAYRNFGDHEALVATHSVNAAGSGTQAGVRWYEVRNPNGTPVI
                     YQQGTYAPDATNRWLGSIAMDQNGNIALGYSASSTSVFPGIRYTGRLAGDPLGQMTQG
                     ESTMMNGSGSQTGTGYRWGDYAMMAVDPSDDCTFWFTTEYIPSTGTAPWRTRIGSFKF
                     PGCGGVAPTPTKTATPGPTATNTPTPTNTPTATPTFTPNPNPPDFSLSAGPASVTIAR
                     RSSGTYTVNLGSLNGFSGNVSLSVSGLPRRTSGAFSPNPV"
BASE COUNT          837 a         1632 c         1531 g          701 t
ORIGIN      
        1 gtccaggcgt acggcagatg ggtcagaccc tcgaccaccg ccagcaggag gctggcgacc
       61 agggcgctat gcgtggcctg caggcgcggc agggccacgc cccgccaggc gatttcttcg
      121 gtattcaaca gaaggcccag acccatattg gccacgaaag cgaacactac ggtccccagc
      181 gataccgtgc cgatgggtaa agcgggcaca ggattgccga gagcgccgta caggccgagg
      241 cccgcgagaa tcagggcggc gatagtcccc agagccacca cataccattg gatgcccacc
      301 cgccagcgca ggaaccggct gaacagagcg cgaaccccgg tcttgccgct gacggccctg
      361 gtcacgatga ccgcggcgat ggcgggcgcc cagccggtag taaacatcaa gatatcgggc
      421 atgtggaagg gcaacaggcc ctgggactcg agggcggtcg ggaccatggt tagccattcc
      481 agcgcgtaga tcaagacaaa caaggacgcc aggggatgct ggctgatgaa ccgtcgaata
      541 gctgatgggc gcgctgccgg cgccacgcgg gtcataacag acacggtgag gttcctttcc
      601 gggagggtcc ccgtactcaa atagctgtac taatcgcttg atccgcggct ttgccggtgc
      661 tgtaacaccg gcgccgcggt tatttgcatg tgttcatgat agcggggaaa tcttcactgt
      721 ggatgagacc aggagcgtga tccgggtgag acaatgtgcc ggttcattag acggcagctt
      781 gggacatatt gtcccggcgg gtcgggacat cgccccagta ggacgaacaa gcggcgacac
      841 tgagggcagc cggcatgagt gatgccgctt ggtattcgcg ctgccgctag gcgcttgccg
      901 gggggcggac gatctgctat gatagcgagc acagcagtac tcccgatcta ctcactcgtc
      961 aataccactt acaggaggca cgcatgggcc tggcgaaggt gcgaagctgt gcgattcgcg
     1021 ggctggacgg cagcctggtc gaggtcgaag tcgatgtcag ccacgggctg cccggctttt
     1081 ttattgtcgg cctgcccgat accacggtgc aggagtcgaa agaacgggtg cgctctgccg
     1141 tgcgcaacag cggcggggtg ttcccggcca agcgcgtggt ggtcagcctg gccccggcgg
     1201 atgtcaagaa ggaaagcagc gcctatgacc tgccgattgc cgtgggcgtg ctggcggcga
     1261 ccgggcaggt gacggcggac ctggatgaca ccgtgttgct gggcgaactg ggcctcgacg
     1321 gcgtgctgcg ccacacgacg ggcatcctgc ccatggtcgc cctggcccgc gaacgagaga
     1381 tccgccaggt ctatgtgccg gaggacgacg ccaacgaagc cgcgctggtg gcgggcatca
     1441 ccgtctaccc ggtgcgcacc ctggcccagt tggtagccca cctgaacagc gtaggcgcgc
     1501 acggcagcaa cggcatcgcc ccggtgccct atcgcgacca cctgttcacg gtggaggacg
     1561 cgggcgccgg gcacgtggac ttgcaggagg tgaagggcca ggaacatgtg aagcgcgccc
     1621 tcgaagtcgc cgccgccggg tcgcacaacc tgctgatgca ggggccgccc ggctcgggca
     1681 agaccctgct ggcgcgctgc ctgccctcga tcctgcccgc catgacgcgc gacgaggcgc
     1741 tggaagtcac caagatctac agcgtggccg ggatgctgcc cgccgggcaa ccgctgatcc
     1801 gcacgcggcc cttccgcgcc ccgcaccaca ccgcgtcgca cgcggcgctg gtgggcggcg
     1861 ggcggcagcc gcgccccggc gagatcagcc tgtcgcaccg cggcgtgctg ttcctcgacg
     1921 aactgctgga gttcgaccag ccgacgctgg agaacctgcg ccagccgctc gaagaccgcg
     1981 tcatcaccat cacccgtgtc tcgggcacgc tgaccttccc ggccagcctg atcctgatcg
     2041 ccgcctgcaa cccgtgcccc tgcggctatt tcggcgacag ccagaaggca tgcgtctgct
     2101 cgcccagcac cgtcactcgc tacaagaaac ggctctccgg cccgctgctc gaccgtatcg
     2161 acatccacgt cgaggtgccg cgcgtggcct acgaaaagct ggcgggcact acgctcggcg
     2221 aatcgtcggc ggcggtgcgc cagcgcgtcg aggcggcacg cgccatccag cgcgcgcgct
     2281 tcgcggggct gcccatcctg accaacagcg agatgcgccc ggcggagatc cgccagttct
     2341 gcgtgctcga cagcgcgggc caggcgctga tcaaggcggc catgcgccag ctcaacctga
     2401 gcgcccgcgg ctaccaccgc gtgctgaaac tggcccgtac catcgccgat ctggcgggcg
     2461 cccagggcat cggcgccgtc cacctggccg aggcgttgca gtaccgggcg cgcgaggggg
     2521 tctgagggcg agcgcgcact cgacattccg aaatcgggca cccgaattgt atgaaattga
     2581 tcctcgccgg ggtcgatttg tcagtacttt tgtcagtacc cttcctgtaa tataggacta
     2641 gcagtggatt tgcccgccgg gcgcggctgg gcaaattgcg attccggttt cagccaagga
     2701 ggaggagtaa tgttgaaacg agtaggcgga tcgcgtatct tccgggccgg ggtggccctg
     2761 gccctgttac tgctggtggc cctcgtgggc gcgcagctta gccacgcccc cgcggcgccg
     2821 gcctggggcg gcgcgcggcc ggccggcctg gccggaggcc cggcagcgcc cggcgccgcc
     2881 ggagatgtgg ccgaagcgcc ggtgactttc ggcgacaacg ggttcgtgtt cgggcgctcc
     2941 gtcaagaatg acgtatcgcc gccgttgcgc tcgatcaagc cggcccctgt gcagcccgcc
     3001 accacgatcc gcgagatgcc cgaaccggcc ggcgaagaca tgagcaaagc cgcacccctg
     3061 gcgccggtga cggacccggt ggtgcagcgc gccttcgggc cggatgtact cgcgccactg
     3121 gcgatgcccg cgccgatcca gaacttcgac ggcgtgaaca acatcgacgg cgtctacccg
     3181 ccggacacca acggcgatgt cggccccaac cactacgtgc agtgggtgaa cctgcacttc
     3241 cagatctggg ataagaacgg caactcgctc tatggccccg ccgcgggtaa caccctgtgg
     3301 agcggcttcg gcggcgcctg ccagtcgcag aacgcgggcg accctgtcgt gctgtacgac
     3361 ccactcgccg accgctgggt gctgagccag ttcacgtcgt ccagccccta cggcgaatgc
     3421 gtcgccgtct cgaccaccgg caacccgacc gggtcctact accgctactt cttccagttc
     3481 agcaccagcg tattctacga ctacccgcac atgggcgtct ggcctgacgg ctactacatg
     3541 agcgccaacc gcttcggcgg cgtcttccag agctaccaag gcgccagcgc catcgtcttc
     3601 gaccgcgcca aaatgctcaa cgggcagccg gccacctacc aggagaagca gaccagttcc
     3661 agctacggca cgctcttgcc gtcggatctc gacgggacca cgccgccgcc cgcgggcgaa
     3721 cctaacttct tcgccgccaa gggcagcacc accctggacc tgtggaagtt ccacgtggac
     3781 tggctcacgc ccggcaattc gagcctcacc ggccccaccc gcctgggcgt ggccgcttac
     3841 aacctgctct gcgccagcac gcgcaactgc gtcaagcaac cgagtacctc ggtcggcctc
     3901 gacggtttgg gcgaccgatt gatgcaccgg ctggcctacc gcaacttcgg cgaccacgaa
     3961 gcgctggtcg ccacccacag cgtgaacgcc gccggttccg gaacgcaggc cggggtgcgc
     4021 tggtacgagg tgcgcaaccc caacggcacg ccggtgatct accagcaggg cacctatgca
     4081 cccgacgcca ccaaccgctg gctgggcagc atcgccatgg accagaacgg taatatcgcc
     4141 ctgggctaca gcgcctccag cacctccgtc ttccccggca tccgctacac gggccgcctg
     4201 gccggcgatc ccctgggcca gatgacgcag ggcgagtcga ccatgatgaa cggcagcggg
     4261 tcgcagaccg gcaccggcta ccgctggggc gactacgcga tgatggccgt ggaccccagc
     4321 gacgattgca cgttctggtt cacgaccgaa tatatcccgt ccaccggcac ggcgccctgg
     4381 cggacgcgca tcgggtcgtt caagttcccc ggctgcggcg gggtcgcgcc cacaccgacc
     4441 aagacggcga cgcccggccc cacggccacg aacacgccca cgcctacgaa cacgcccacg
     4501 gccacgccga cgtttacgcc caacccgaac ccgcccgact tcagcctgag cgcaggcccc
     4561 gccagcgtga ccatagcgcg ccgcagcagc ggcacctaca ccgtgaacct gggctcgctc
     4621 aacgggttca gcggcaatgt cagcctgagc gtgagcgggc tgccgcggcg aaccagcggc
     4681 gcattcagcc cgaaccccgt c
//
LOCUS       DASUYC010000957         3382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_2007090, whole genome shotgun sequence.
ACCESSION   DASUYC010000957 DASUYC010000000
VERSION     DASUYC010000957.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2007090"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..639)
                     /locus_tag="VF276_15645"
     CDS             complement(49..639)
                     /locus_tag="VF276_15645"
                     /inference="COORDINATES: protein motif:HMM:NF014845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-vinyl reductase"
                     /protein_id="HEX6608334.1"
                     /translation="MTGDINGTTMRVVLESLKTVSGPQYEMLLQRAGLDRFLKELPAA
                     DGKTAATGEELVRLFTVVYMMLGEVCTRLFHRNCGVAYADGIMRSGWGDKMQAKAATI
                     PADQKLPWVVRELIALAEHSYSPQIVSEDAAAWYLASATCYVCLGLQGVKAPICTSTE
                     TMYKELAKKLVGRTVRVVEVDCAALGASHCKFAIYK"
     gene            complement(689..1021)
                     /locus_tag="VF276_15650"
     CDS             complement(689..1021)
                     /locus_tag="VF276_15650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608335.1"
                     /translation="MEEGKGPAVMDERDGVSISQVLRPVNTPRDVEELTRMQIARLHT
                     IVQPLFEDLPLAALSASSEQTSEEQQAVDRAYTHYITAEQLLNAALRVYASLLAAGAN
                     RHGPDSPA"
     gene            1320..1979
                     /locus_tag="VF276_15655"
     CDS             1320..1979
                     /locus_tag="VF276_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002625866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX6608336.1"
                     /translation="MTINELATRAGVTTRTIRYYVEQGLLPAPERGRPAEYTEDHVQR
                     LDLIRRLKDQYLPLEEIRAMLERLDRKEIEQVLAPAPPVAAARARTSAVDYIADVLGR
                     GATREKLKTQPPPRPLPPGMAPPPASLPPDWAPPVPLPPAPAAVPPPAPAPQTLAPPQ
                     YLPAIEPAPTTWQRVPLAPGVELHYLMSGDARTNELVDRLVEAAGRILAEMPESNGEK
                     L"
     gene            1979..3226
                     /locus_tag="VF276_15660"
     CDS             1979..3226
                     /locus_tag="VF276_15660"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX6608337.1"
                     /translation="MMKLLAHLDRRLVPPEGTDARYLRVQLVAPDRPHATDRLPLNLA
                     LVLDRSGSMAGSKLDKAKEAAIFCLRNLTAADRVAVVAYDDEVQVVAPSRALTPSVKT
                     GLIAEVRKIESGGSTNLGGGWLTGAQEVANYQHEANYLSRTILLSDGLANVGITDPDE
                     LAHHAGELRARGVSTTTMGIGADFQEDLLEQMALRGGGHFYFIEDARQIPDFLHRELG
                     EVLSTCARQVVVTLDAPDGGNARLLNAFESERDGGLRVRLDDMIAGETRSVVFKLAVP
                     AGREGSPLPIRVRLSYLDVDSGETRTVQSGEAVLTYADAAACAAEAPDTSVVEEVALL
                     QVARAREEALRYDAAGQYAQSAATLAQAATYLRAAAPASPMAQAEAQALDADSTEAQR
                     GFGQLKRKALHYAKATRLQNRNQ"
BASE COUNT          558 a         1153 c         1138 g          533 t
ORIGIN      
        1 ccgatcgctg ggcgatcggg gcgtaccttt tgccggtagg agccacaact acttataaat
       61 agcgaatttg cagtgtgagg cacccagggc ggcgcaatcg acctccacga cccggactgt
      121 gcggcccact agcttcttgg cgagttcctt gtacatcgtt tcggtggagg tacagatggg
      181 cgccttgacc ccctgcaggc ccaggcagac gtaacaggtg gcgctggcga gataccaggc
      241 cgctgcgtct tcgctgacga tctgcggcga gtaggagtgc tcggccagcg cgatcagctc
      301 ccggaccacc caggggagtt tctgatcggc gggaatcgtg gccgccttgg cttgcatctt
      361 gtcgccccag ccgctgcgca taatgccgtc ggcataggcc acgccgcagt tgcgatgaaa
      421 gagccgggtg cagacttcgc ccagcatcat atagacgacc gtaaagagcc ggaccagttc
      481 ttccccggtc gcggcggtct tgccgtccgc cgccggcaac tccttcagaa agcgatccag
      541 ccctgcccgc tgcaacaaca tttcatactg cgggccgctc acggttttta acgattccag
      601 tacgacccgc atggtcgtgc cgttgatgtc cccggtcatt tttaccccta tgttgagacg
      661 tgtgagtcgg tcgtgtcgtt ccgccggctt acgccgggct gtccgggcca tgccggttcg
      721 cgcccgccgc cagcaggctg gcgtacacgc gcagcgcggc gttcaaaagc tgctcggcgg
      781 tgatgtagtg tgtgtaggcg cggtccaccg cctgctgctc ctccgaggtt tgctcggacg
      841 aggcgctgag cgcggccagc ggcagatcct cgaatagcgg ctgcacaatg gtatgcagcc
      901 gggcaatttg catgcgcgtc aattcttcaa cgtcgcgcgg cgtgtttacc gggcgcaaca
      961 cctgactaat cgatacccca tcccgttcgt ccatcactgc cggtcccttc ccttcttcca
     1021 tccttactcc accgggcctg cctggcggcg cctgtgccgc accgagccgg gcattactgc
     1081 ccggtcaggg tcaacagcgc aaaacaaccg gccccggcgc ggggctgatc ccttctccat
     1141 catactccat tccgggcgct gtggacagac agcgaggtcc cttagagcca gggaccgcta
     1201 gcccgatccc gtaggacctt gctacttgaa ttgatccgca aattggttcc gccaacatat
     1261 tgacagtatt acgtaatagt gctatgatac cagtgttacg tgacgatggg agataagaca
     1321 tgacgatcaa cgaactggcg acccgcgcgg gcgtgaccac gcgcacgatc cgctactatg
     1381 tcgaacaggg gctgctcccg gcgccggagc gcgggcggcc cgcggaatat accgaggacc
     1441 atgtccagcg gctggacctg atccggcgct tgaaagacca gtatttgccg ctggaggaga
     1501 tccgggccat gctggaacgg ctggatcgca aggagattga gcaagtgctg gcgcccgcgc
     1561 cgcccgtggc tgccgcgcgg gcccgaacct cggcggtgga ctatattgcc gatgtgctgg
     1621 ggcggggcgc gacgcgcgag aagctgaaga cgcaaccgcc gccgcgcccg ctgccgccgg
     1681 ggatggcgcc gccaccggcc agcctgccgc cggactgggc gccgcccgtg ccgctgccac
     1741 ccgccccggc cgccgtgccg ccgcccgccc cggccccgca gacgctggcg ccgccgcagt
     1801 atctgccggc tatcgagccg gcgccgacaa cctggcagcg ggtgccactg gcacccggcg
     1861 tggagctaca ctatctgatg agcggcgatg cccgcacgaa cgagttggtg gaccggctgg
     1921 tcgaagccgc cggtcgcatt ctggccgaga tgccggagag taatggagag aaactctgat
     1981 gatgaagtta ctggcgcacc tggaccgccg gctcgttccg cccgagggca ccgatgctcg
     2041 ttatctgcgc gtgcaactgg tcgcccccga tcgcccgcac gcgaccgacc gcctgccgct
     2101 caacctggcc ctcgtgctcg accgcagcgg gtcgatggcg ggcagcaagc tcgataaggc
     2161 caaggaagcg gcgatcttct gcctgcgcaa cctcaccgcc gccgaccgcg tggccgtggt
     2221 ggcctacgac gacgaggtgc aggtcgtggc gcccagccgc gccctgaccc cgtccgtcaa
     2281 gacgggcctg atcgccgagg tgcgcaagat cgagagcggc ggcagcacca acctgggcgg
     2341 cggctggctg accggcgcgc aggaagtggc gaactatcag cacgaggcta attatctgag
     2401 ccggacgatc ttgctgagcg acgggctggc gaacgtcggc atcaccgacc cggacgagtt
     2461 ggcgcaccat gcgggcgagc tacgggcgcg cggcgtgagc accacgacca tgggcatcgg
     2521 cgccgacttc caggaggatc tgctggagca gatggcgctg cgcggcggcg gccacttcta
     2581 cttcatcgag gacgcccggc agatccccga ttttctgcac cgggaactgg gcgaagtgct
     2641 gtccacctgc gcccgccagg tggttgtcac gctcgacgcg ccggacggcg gcaatgcccg
     2701 cctgctcaac gcctttgaga gcgagcgcga cggcggcttg cgggtgcgcc tcgacgacat
     2761 gatcgccggg gagacgcgct cagtggtgtt caagctggct gtgccggcgg gccgggaggg
     2821 ctcgccgctg ccgatccggg tacggctgag ctatctcgac gtggacagcg gcgagacgcg
     2881 caccgttcag agcggcgagg cggtgctcac ctatgccgat gcggcggcct gcgcggcgga
     2941 ggcgcccgat acgagcgtgg tcgaggaagt ggcgctgctc caggtggcgc gggcgcgcga
     3001 ggaagccctg cgctacgatg cggcgggcca gtacgcgcag agcgcggcca cgctggccca
     3061 ggccgcgacc tacctgcgcg ccgccgcccc ggcgtcgccc atggcccagg ccgaggcgca
     3121 ggccctcgac gccgacagca ccgaggcgca gcgcggcttc ggccagctca agcgcaaggc
     3181 gctgcattac gccaaagcca cccgcctgca gaaccgcaac cagtagccgg gcccgccccg
     3241 gcttacgcgc ccggcccgcc cgacgccagc gcctgcaacc gctggagccg ggcgggttcg
     3301 tgctcggcca gccacgcatc ctgggccagg gcggcgtagg cccgcgcgaa ataggcgcgg
     3361 gcggcagcgg gccggccctg gg
//
LOCUS       DASUYC010000958         3667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1313404, whole genome shotgun sequence.
ACCESSION   DASUYC010000958 DASUYC010000000
VERSION     DASUYC010000958.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1313404"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1238
                     /locus_tag="VF276_15665"
     CDS             <1..1238
                     /locus_tag="VF276_15665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6608338.1"
                     /translation="ALPHRAVFRAAAQAEAGRVNILYVASAIALPGTSGGATHVREVA
                     EGLARRGHRVHVVARRGQGGAADLGPGIESTLLPWPDKLALLGYPAVAGVARRFRPDV
                     VMERYYNFAGAGVLLAHRRGLPALLEVNAPMVDPPGSHKETLDRLLGRPLRRWAVRQA
                     RWSRRIVTPLASTVPPPVPRERIEQVNWGANVDRFRPGLREECAAELAALRRAWGIPE
                     GGTVAAFAGSFRAWHGVQSFVRAALQLIPDHPDLYFLAVGGGPELAPVQALAGESGQA
                     AARRIIFTGAQPHERMPLLLALADIGVAPFVPAAYPPLRAFGFYWSPLKVFEYMAMGQ
                     PVVTPAIPPLDGIIRAGQEGLLYPEGDEAALAQAIGALAADPAARGAMGARARERVVA
                     CYSWDAHCAQLEQALEAIR"
     gene            1235..2584
                     /locus_tag="VF276_15670"
     CDS             1235..2584
                     /locus_tag="VF276_15670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6608339.1"
                     /translation="MKILIATDVFPPRCGGAGWSTYYLARALRACGHEVSIVRPRFDA
                     TARQPRVHPVEYDGFRVAEAVLPLPQNPPLRLIARNIRGPRLLRRLIAREATRLGADL
                     IHAQHALTTPAAVGAADDLARGRQARRVPVVSTVRDYWPLCYYNTMQVPRRIAVPGAG
                     TPPSAFVPPRDVPVARSLARALWRAQGRRAVRSLPLLPLWQIATARRRRALRRADAVI
                     AVSGYVADRLRRGGAVAPDRLHVIPNLVDRPRLEALAAEPPPLAAFDLAPGAPFLLFV
                     GKLEPSKGAQLLPRALQAAGVGASLPLVVAGSGPLQPWLAAAGPAHGLDIRFGAWLDN
                     ADVLRLMRAATLLLFPSDWAEPLSRVLLEGCAAGAAILALDTGGTPDIIADGQNGRLV
                     ADMNAFAAALRELLDDPTQRARLAAGAQATARECFSADVVAAQVEALYEALLAGSAA"
     gene            2581..>3667
                     /locus_tag="VF276_15675"
     CDS             2581..>3667
                     /locus_tag="VF276_15675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6608340.1"
                     /translation="MTTHPAGGAPLRVGHLVRAIFPQHGYGGMERAATALTDHMLRAG
                     ADVTLFTRTWPGMGDRLALAPAERRACVVGVPYSVLPLRPNSILARLTNYPLFVERMG
                     RAVGNRARAGALDIVYAHGLCAYGVRSRDRRRTPRGVAPLVMNPHGLEDFKVRHPLKR
                     LAYAPFRAMYARGAQAADWVIATDESLRGEVSRYLRVPPERIVVLPNGVDPEAGAALV
                     SAGRQAALRRRYGLGPETVVGLSVARLEANKGLSYLLRALAALDRERPWRWVLVGTGR
                     DRAVLEALAGELGLGDRLIFAGAVDDADLHNLYALAGVFAISSLYEGSSLATLEAMAH
                     GLPVVATRAGGLPDKVLPERTGLLVPPG"
BASE COUNT          409 a         1298 c         1426 g          534 t
ORIGIN      
        1 gggcgcttcc gcaccgggcc gtatttcgtg ctgctgcgca agccgaagcc gggcgcgtga
       61 acatcctcta cgtggcctcg gctatcgccc tgcccggcac gtcgggcggc gcgacccacg
      121 tgcgcgaagt ggccgaaggg ctggcgcggc gcggccatcg cgtccacgtc gtggcccggc
      181 gcggccaggg cggggcggcg gatctcggcc ccggcattga gagtacgctg ctgccctggc
      241 ccgacaaact ggccctgctc ggctacccgg ccgtcgccgg ggtggcgcgg cgcttccggc
      301 ccgacgtggt gatggagcgc tactacaact tcgcgggcgc gggcgtgctg ctggcgcacc
      361 ggcgcggcct gcccgctctg ctcgaagtga acgcgccgat ggtcgatccg cccggttcgc
      421 acaaggagac gctggaccgg ctgctggggc gcccgctgcg ccgctgggcc gtgcgccagg
      481 cgcgctggag ccgccgcatc gtcaccccgc tggcgagcac cgtgccgccg ccggtgccgc
      541 gcgagcggat cgaacaggtc aactggggcg ccaacgtgga ccgcttccgc cccggcctgc
      601 gcgaggagtg cgccgccgaa ctggccgcgc tgcgccgcgc gtggggcatc ccggaagggg
      661 gcaccgtcgc cgcgttcgcc ggcagcttcc gcgcctggca cggcgtccag agtttcgtgc
      721 gcgccgcgct ccaactcatc ccggaccatc ccgacctcta tttcctggcc gtgggcggcg
      781 ggccggaact ggccccggtg caggcgctgg ccggcgagtc ggggcaggcg gcggcgcggc
      841 gcatcatctt caccggggcg cagccgcatg agcggatgcc gctgctgctg gccctggccg
      901 atatcggcgt ggcgccgttc gtgcccgccg cctatccgcc cctgcgcgcc ttcggcttct
      961 actggtcgcc gctcaaggtc ttcgagtaca tggcgatggg ccagccggtg gtgacgcccg
     1021 cgatcccgcc gctggacggc atcatccgcg ccgggcagga ggggctgctg taccccgaag
     1081 gcgacgaggc ggcgctggcc caggcgattg gcgccctggc cgccgatccc gcggcgcgcg
     1141 gcgcgatggg cgcgcgcgcc cgcgagcggg tggtagcctg ctatagctgg gacgcccact
     1201 gcgcgcaact ggagcaggcc ctggaggcga tccgttgaag atcctgatcg ccaccgatgt
     1261 cttcccgccc cgctgcggcg gcgccgggtg gagcacctac tacctggcgc gggccttgcg
     1321 ggcgtgcggg cacgaggtga gcatagtccg cccgcgcttc gacgccacgg ctcgacaacc
     1381 acgggtgcat ccggtagaat atgatgggtt tcgtgtggcc gaagcggtgc tgccgttgcc
     1441 gcaaaacccg ccgctgcgcc tgatcgcgcg caatatccgg gggccgcggc tgctgcgccg
     1501 gctgatcgcg cgggaggcca cgcggctcgg cgcggacctg atccacgccc agcacgcgct
     1561 gaccacgccc gcggcggtcg gcgcggcgga cgacctggcg cgggggcgac aggcgcggcg
     1621 ggtgccggtg gtcagcaccg tgcgcgacta ctggccgctc tgctactaca acacgatgca
     1681 ggtgccgcgc cgcatcgccg tgccgggtgc cggcaccccg ccttccgctt tcgtccctcc
     1741 ccgcgatgtg ccggttgccc gcagcctggc gcgggcgcta tggcgggcgc aagggcggcg
     1801 ggccgtgcgc agcctgccgc tgctgccgtt gtggcagatc gccaccgcgc gccgccgccg
     1861 ggcgttgcgg cgggccgatg ccgtgatcgc cgtcagcggc tacgtcgccg accggctgcg
     1921 ccggggcggg gccgtcgcgc ccgaccggct gcacgtcatc ccgaacctgg tggatcggcc
     1981 ccggctggaa gcgctggccg cggagccgcc cccgctggct gcgttcgacc tggcgccggg
     2041 cgcgcccttc ctgttgttcg tgggcaaact ggagcccagc aagggcgcgc aattgctgcc
     2101 gcgggcgttg caggcggcgg gggtgggggc gagcctgccg ctggtggtcg cgggcagcgg
     2161 gccgttgcag ccctggctgg ccgccgccgg tccggcccac ggcctcgaca tccgcttcgg
     2221 cgcctggctg gacaacgccg acgtgctgcg gcttatgcgc gcggccacgc tgctgctgtt
     2281 cccctccgac tgggcggagc cgctgagccg ggtgctgctc gaaggctgcg cggccggcgc
     2341 ggccatcctg gccttggaca ccggcggtac gcccgacatt atcgccgacg ggcagaacgg
     2401 gcgcctggtg gccgatatga acgccttcgc ggcagcccta cgcgagttgc tggacgatcc
     2461 cacccaacgc gcgcggctgg cggcgggggc gcaggccacg gcgcgggagt gcttcagcgc
     2521 cgatgtggtc gcggctcagg tcgaggcctt gtacgaagcg cttctcgccg gaagtgcggc
     2581 atgacgacgc atccggcggg cggcgcgccg ctacgggtgg gccacctggt acgggcgatc
     2641 ttcccgcagc atggctacgg cggcatggag cgcgcggcga cggcgctgac cgatcacatg
     2701 ctgcgggcgg gcgccgacgt gaccctgttc acgcgcacgt ggccgggcat gggcgaccgg
     2761 ctggccctgg ccccggcgga gcgcagggcc tgcgtggtgg gcgtgcccta ttcggtgctg
     2821 ccgttgcggc ccaactcgat cctggcccgg ctgacgaact atccgctctt tgtcgagcgc
     2881 atgggccggg ccgtgggcaa ccgggcgcgg gccggtgcgt tggatatcgt gtacgcgcac
     2941 gggctgtgcg cctatggcgt gcgtagccgg gatcggcggc ggacgccgcg cggcgtggct
     3001 ccgctggtga tgaacccgca cggcctggag gacttcaagg tccgccaccc gctgaagcgg
     3061 ctggcctacg cgcccttccg cgccatgtac gcgcggggcg cccaggccgc cgactgggtg
     3121 atcgccaccg acgagtcgct gcgcggcgag gtgagccgtt atttgcgtgt gccgccggag
     3181 cgcatcgtcg tgctgccgaa cggggtggac ccggaggcgg gcgccgcgct ggtctcggcg
     3241 gggcggcagg cggccttgcg gcggcgttat ggcctgggac cggagacggt cgtgggcctc
     3301 agcgtggccc ggctggaggc gaacaagggg ctaagctacc tgctgcgcgc cctggccgcg
     3361 ctcgaccggg agcggccctg gcgctgggtg ctggtcggca cggggcgtga ccgggcggtg
     3421 ctggaagcgc tggccgggga gctgggcctg ggcgaccggc tgatctttgc cggggccgtg
     3481 gacgacgccg atttgcataa tttgtatgcc ctggccgggg tgttcgccat ttcgagtctg
     3541 tacgaaggca gttccctggc gaccctggag gcgatggccc acggcttgcc ggtggtggcg
     3601 acgcgggccg ggggcctgcc tgacaaggtc ttgccggagc gcaccggcct gctagtgccg
     3661 cccggcg
//
LOCUS       DASUYC010000959         2537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_669022,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000959 DASUYC010000000
VERSION     DASUYC010000959.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669022"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="VF276_15680"
     CDS             complement(<1..849)
                     /locus_tag="VF276_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009627020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6608341.1"
                     /translation="MAAGNRRRRNWRRIGLIVLISGFVLLNGVAWMQTWAMTHFAPAG
                     QPPPRMESLSAPQKAWLVMTGVQVARPRNEHTPRDMGLAYEVRRIPVGNGESLEAWWM
                     PQTQPRGLVLLFPGYASPKESLLARALVFHDLGYDALAVDFRGVGGSSGQDTTLGVRE
                     ATDVAQAVAYARQTWPDRRLVLYGVSMGSAAVLRAVAQAGVRPAAVILESPFDRLLTT
                     VRNRFRVMGLPAFPSAELMVFWGSVQHGFNGFAHNPADYAAAVQCPALVLHGDQDTRA
                     TTAEARA"
     gene            1030..1353
                     /locus_tag="VF276_15685"
     CDS             1030..1353
                     /locus_tag="VF276_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEX6608342.1"
                     /translation="MIIATTENVAGHRVVETKGTVFGVVVRSRSIGGNVVANLRSLGG
                     GEITEYTQLLDETRRQAVNRMVDKALAMGANAIIMMRFDSSEIGTFMSEIVAYGTAAV
                     VQPVG"
     gene            complement(1416..1856)
                     /locus_tag="VF276_15690"
     CDS             complement(1416..1856)
                     /locus_tag="VF276_15690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608343.1"
                     /translation="MALKPHDINLAHELDPAWIIALWLAIHGGDPRPDEQTVSETAER
                     AIAAQVIAELAPFVGGAAGHAIAEVLQPLHNGHGAKLAKGLTLHHLEAAMKALHVEVR
                     VHEAQAGEARDLQAIHRQYCFRFQGRTICVPVGPQVAHPPVTHL"
     gene            2132..>2537
                     /locus_tag="VF276_15695"
     CDS             2132..>2537
                     /locus_tag="VF276_15695"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX6608344.1"
                     /translation="MPRATTPAGRGMVPPAVGIDIGTCFARVAVLDGDGPRLVADRYG
                     DRACPAVVRYGMHGSDVGYYPERFLVTDWENTVREVPRFLGRYADLPRRVIEAAPFPV
                     YEEEGRARFNLLYTTAGPEEVYTRLVRHLAGLA"
BASE COUNT          403 a          842 c          904 g          388 t
ORIGIN      
        1 ggcgcgcgcc tcggcggttg tggcgcgggt gtcctggtcg ccgtgcagca ccagggccgg
       61 gcactggacg gcggcggcgt agtcggcggg gttgtgggcg aagccgttga agccgtgctg
      121 cacgctgccc cagaagacca tcagctcggc actggggaag gcgggcaggc ccatcacccg
      181 gaagcgattg cgcaccgtag tcagcagccg gtcaaaggga ctctccagga tgacggcggc
      241 gggtcgcaca ccggcctggg cgacggcgcg caacaccgcg gcgctgccca tggacacccc
      301 gtagagcacc agccgccggt cgggccaggt ctgccgggcg taggccaccg cctgggccac
      361 gtccgtcgcc tcgcgcacgc ccagcgtggt gtcctggccg ctcgacccgc ccacgccgcg
      421 aaagtccacc gccagcgcgt cgtagcccag gtcgtggaag accaacgccc gcgccagcag
      481 gctctccttg ggcgacgcat agccggggaa cagcagcacc agcccgcgcg gctgtgtttg
      541 gggcatccac cacgcctcca ggctttcgcc gttacccacg gggatgcgcc gcacctcgta
      601 ggccaggccc atgtcgcggg gcgtatgctc gttgcgcgga cgggccacct gcaccccggt
      661 catcacgagc caggcttttt gcggggccga caggctctcc atacgtggcg gcggctgccc
      721 ggcgggcgcg aagtgggtca tggcccaggt ctgcatccag gccaccccat tcagcaacac
      781 gaatccgctg atgaggacga tcaagccgat ccgccgccag ttgcgccgtc gccgattccc
      841 cgccgccacc ctatctcctc tccgcccaat cacgcctcaa tgataccatg cgccagccag
      901 actcgtatat accgggcgaa cgaacgcgcc gggcgccggg gcgcgcccaa gctacttttg
      961 cgctgtaggg gccggtttga aaccggcccg gcgaccggcg gcagagcggg aatcgaaagg
     1021 agccagagca tgatcatcgc gacgacggag aatgtggcgg gccaccgggt ggtggagacc
     1081 aaggggaccg tgtttggcgt ggtggtgcgc agccgcagca tcggcggcaa tgtggtggcg
     1141 aatctgcgct cgctgggcgg cggggagatc accgagtaca cgcaactgct cgacgagaca
     1201 cggcggcagg ccgtgaaccg catggtcgac aaagccctcg ccatgggcgc caacgccatc
     1261 atcatgatgc gcttcgactc gtcggagatc ggcacgttca tgagcgagat cgtggcctac
     1321 ggcaccgccg ccgtggtgca accggtcggc tgaggcggcg cgggggaccg gggacggacc
     1381 ggcggcccgc ccccggcatg tgtatggacc gcgccctaca gatgggtgac gggcgggtgg
     1441 gcgacctgcg gtcccaccgg cacgcagatg gtgcgaccct ggaagcggaa gcaatactgg
     1501 cggtgaatgg cctggagatc gcgcgcttcg ccggcctgcg cctcgtggac gcgcacctcc
     1561 acatgcagcg ctttcatggc cgcttcgaga tggtgcagcg tcagtccttt cgccagtttc
     1621 gcgccgtggc cgttgtgcag cggctgcaag acttcggcaa tggcgtgacc cgccgcgccg
     1681 cccacgaaag gcgccagttc cgcgatgacc tgggcggcga tggcgcgttc ggcggtctcg
     1741 ctcaccgtct gctcatcggg gcgcgggtcg ccgccgtgga tcgccagcca gagggcgatg
     1801 atccaggccg ggtcgagttc gtgggccagg ttgatgtcat ggggtttgag tgccacaggt
     1861 aaggtctcct tcgtgctggg gttggccgcg tccaggtgcc gtgttgcgcg ggatccacgc
     1921 gctgtaacat gtcactcatc cgttgtatgg gcgccggggc gatcccggtt attagccggg
     1981 accaatttcg gccaacctct tgacagcgcc gggaaacggt gctatagtta gtgtatcaga
     2041 tacaataaag gctgcgggac agggaccaac cccggcagcc gctttttagg gccgccagtt
     2101 agtgtagtta tgacattaag gagaacgagc gatgccacga gcgacgacac cggcggggcg
     2161 gggcatggtg ccccccgccg tcggcatcga catcggcacc tgcttcgcgc gggtggccgt
     2221 gctggacggc gacgggccgc ggctggtggc cgaccgctac ggcgaccggg cctgcccggc
     2281 ggtggtccgc tacggcatgc atggcagcga cgtgggctac tacccggagc gcttcctggt
     2341 gaccgactgg gagaacacgg tgcgcgaggt gccccgcttc ctgggccgct acgccgacct
     2401 gccgcgccgg gtgatcgagg cggcgccctt cccggtgtac gaggaagagg gccgggcgcg
     2461 cttcaacctg ctctatacca ctgccgggcc ggaggaggtc tatacccgcc tggtgcgcca
     2521 cctggccgga ctggcaa
//
LOCUS       DASUYC010000960         4637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_216089,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000960 DASUYC010000000
VERSION     DASUYC010000960.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216089"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            530..1789
                     /locus_tag="VF276_15700"
     CDS             530..1789
                     /locus_tag="VF276_15700"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6608345.1"
                     /translation="MKNPRAYRVYLFMEAASSFCLTTIFLLAAVYRLQTVHLDALQLV
                     LVGTVLELSCFVFEVPTGVVADSYSRRLSVILGFVFIGVAELLEGSIPVFAAVLLAQV
                     VSGLGYTLLSGATEAWIAGEVGEEHLTHVFQRGGQMGQVGNLLGIGAAVGLGSVGLNW
                     PFLVGGMGMIGLGLVLAFIMPETGFAPTPRGERTTWQHMGATFRDGLGVIRTRPVLLS
                     FLGIAVFFGMTSEGIDRLWEAHLLTNFTFPALGGLTSLVWFGLISVAGSLLYLAGTEV
                     LRRRVNLDRPGAVVRALIVLNVFAVAALILFGVADSFALALAAFLVYGLCRGLGGPLH
                     GAWLASSIDPRVRATVLSMISQVDAVGQIAGGPVIGAVGKAFSIRAALVLAGALLAPT
                     LPLLARAGRRARASQGPAEGPVPVAEA"
     gene            complement(1831..2703)
                     /locus_tag="VF276_15705"
     CDS             complement(1831..2703)
                     /locus_tag="VF276_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEX6608346.1"
                     /translation="MITWFTDPLQYGFMQRALLEVILMGLTCGVIGAYVVLRGMAFLG
                     DALAHAIFPGVVIAFLLNINLFAGALVFGILTVIGIAGIARNRRVSEDSAIGVLFTGA
                     FALGVVLISQVKGYTGNLADFLVGQVLGVSTTDVLISVAVAVLVLGALAFFNKELVLT
                     AFDREMAEAMGLPVALLDMGLLLGIALTIIVSLQAVGNILVVAMLVTPAAAARLWTDR
                     LPVMMALSALFGILAGIGGLLVSYHGNFAAGGTIVLLVTLLFGVSYVFAPRHGWLTRR
                     RLARSPAPAQQNPA"
     gene            complement(2719..3657)
                     /locus_tag="VF276_15710"
     CDS             complement(2719..3657)
                     /locus_tag="VF276_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEX6608347.1"
                     /translation="MIGQTAHPPAAAARPTAQQAPAAVLRDVTVGYDAPPVLEKVNLT
                     LDWGLIVGAIGPNGAGKSTLIKTILGILPARSGEVRVAGQPVASGAARRTMGYVPQRE
                     AVNWDFPVTVADVVLMGRTACLGWGRRPGPADERLAHEALVQVGMQDFAHRQISQLSG
                     GQQQRVFLARALVQEGDLLLLDEPLNGVDASTQEVIGTLLRQQRAAGHTVVMATHDLE
                     LAAEWCDRIVLVNHGIVCYGPPAEVLTPDMLRQTYGGQTLLVPEEFAGHGATLLVPDE
                     HGHGGGHSHPHTHSHDHHHHHLPANDPLCRDPEATQ"
     gene            complement(3654..>4637)
                     /locus_tag="VF276_15715"
     CDS             complement(3654..>4637)
                     /locus_tag="VF276_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEX6608348.1"
                     /translation="PLVTRNQEAMLYHKRGRRVGLIVALAVLTALLSACGDSPAPSAT
                     PGTPGPPPAGGRVLHVTVTTTQIRSMADAVAGNAAKVTPILQASDDPHAYEPKPSDVQ
                     LIANADLVLRHGMGLDHWLDKVVQNAGGNHPVVTVTTGVKLESGDGEESGPDPHVWFD
                     VTNAMTMTRDIRDAMIAADPANKDAYQANADAYLATLKQLDSDIMSQIATIPPDQRKM
                     VTNHDAFGYYIRRYGLTFVGSVVPSMDSSAEPSAKQLAELIDKIKAEHVRAIFLESSI
                     NPKLEKQIAAEAGVNVVDTLYGDTLGPPNSPAYTYEGMMRYNTQTIVRALK"
BASE COUNT          650 a         1494 c         1691 g          802 t
ORIGIN      
        1 tcatcgcgcc tgccacccta ccgcgatctg gcgcgtagcc gtgtgccgcc cgtcgagcat
       61 ccgggtgcgg cgacgggcgc gattcatcgc gccgctacgg agggcggggc ggcagatggc
      121 ggatcgggaa atacgcctgt agacccatgc ggggcggcgc cgatggcggt acactggggc
      181 agtatctcgc ggcgccgggc ttccggtgct gcctgttggt tcggttcgtg gaaaggatca
      241 acgtcatgca aatttctatg ctagtcctga tgccgatgaa atcgctcgtg atccgcgcgg
      301 ggcgcggaga cggcgcgcgg ggcacggtct agcagttcga ccggttcgcc ggtcgcttgc
      361 atttgccagg cacgggtcgc cgtcgcctgc ctctctgtgt cccctccgcc cgccgtgggc
      421 gcggcccgat gccgcgtccc gcccctgcca tgcctccgct gacccgtggg tctcacgagc
      481 atctctagtc tgattctgca gccgcctggg ctgcactaag gagatacttg tgaagaatcc
      541 gcgcgcgtat cgcgtgtacc tgttcatgga ggccgcgtcc tccttttgcc tgaccaccat
      601 cttcctgctg gccgccgtct accggctgca aaccgtccac ctggacgcgc tgcaactggt
      661 gctcgtcggc acggtgctgg aactgtcgtg cttcgtgttc gaggtgccga cgggagtggt
      721 ggccgatagc tatagccgcc gcctctcggt gatcctcgga tttgtgttta tcggcgtggc
      781 ggagctgctg gaaggctcca tccccgtgtt tgccgccgtg ctgctggcgc aggtggtttc
      841 cgggctgggc tacaccctcc tcagcggggc gaccgaagcc tggatcgccg gcgaagtggg
      901 cgaggaacac ctgacccacg tgtttcagcg cggcgggcag atggggcagg tgggcaacct
      961 gctgggcatc ggcgcagcgg tgggcctggg cagcgtgggc ctcaactggc cgttcctggt
     1021 tgggggtatg gggatgatcg gcctgggcct ggtgctggcg ttcatcatgc ccgaaaccgg
     1081 cttcgcgccg accccgcggg gcgagcgcac aacctggcag cacatggggg ccaccttccg
     1141 cgacgggctc ggcgtgatcc gcacccgccc ggtgctgctc agcttcctgg gcatcgccgt
     1201 cttcttcggc atgaccagcg agggtatcga ccggctctgg gaggcgcatc tgctgaccaa
     1261 cttcaccttc cccgcgctgg gcgggctgac ctcactggtc tggttcggct tgatcagcgt
     1321 ggccgggtcg ctgctctatc tggccgggac cgaagtgctg cggcggcggg tcaacctcga
     1381 ccggccgggg gcggtggtgc gggcgctcat cgtgctgaac gtgttcgccg tcgcggcact
     1441 gatcctgttc ggcgtcgccg acagtttcgc cctggccctg gcggcgttcc tggtctacgg
     1501 cctctgccgg gggctgggcg ggccgctcca tggcgcctgg ttggcgagca gcatcgaccc
     1561 aagggtacgc gccaccgtgc tctcgatgat cagccaggtg gacgccgtgg ggcagatcgc
     1621 cggagggccg gtgatcggcg cggtgggcaa ggcgttctcg atccgggcgg cgctggttct
     1681 ggctggggcg ctgctggcac ccacgctgcc cctcctcgcg cgcgccgggc ggcgggcgcg
     1741 ggcgagccag ggcccggcgg aagggccggt gcccgtggcg gaagcataac gactggcggg
     1801 gccggttcgc cgtgcgcggg ccggcccctg ctatgccgga ttttgctggg ccggtgccgg
     1861 gctgcgcgcc agccggcggc gggtgagcca gccgtggcgc ggggcgaaca cgtaggagac
     1921 gccgaacagc agcgtgacca gcagcacgat ggtgccgccc gccgcgaagt tgccgtggta
     1981 ggagacgagc agcccgccga tgccggccag gatgccgaac agggcgctga gggccatcat
     2041 caccgggagg cggtcggtcc agagccgggc cgcggcggcc ggcgtgacga gcatagcgac
     2101 gaccaggatg ttgccgacgg cctgcagcga cacgatgatg gtcagcgcga tgcccagcag
     2161 caggcccatg tcgagcagcg ccaccggcag gcccatcgcc tcggccatct cgcggtcgaa
     2221 cgcggtcagc accagttcct tgttgaagaa ggccagcgcg cccagcacca gcacggccac
     2281 cgccacgctg atcagcacgt cggtggtcga gacgcccagc acctggccca ccaggaaatc
     2341 ggccaggttg cccgtgtagc ctttgacctg gctgatcagc acgacgccga gggcgaaggc
     2401 gccggtgaac agcacgccga tagcgctgtc ctcagagacg cgccggttgc gcgcgatgcc
     2461 ggcgatgccg atcacggtca ggatgccgaa gacgagcgcc ccggcgaaga ggttgatatt
     2521 cagcaggaag gcgatgacca cgccgggaaa gatggcgtgg gccagggcat cgccgaggaa
     2581 ggccatgccc cgcagcacga cgtaggcgcc gatgacgccg caggtcagcc ccatcaggat
     2641 gacctccagc agcgcgcgct gcatgaaacc gtattgtaag ggatcggtga accaggtgat
     2701 cacagcgcgg gcctccggtt attgggtcgc ttccgggtcc cggcagagcg ggtcgttggc
     2761 gggcaggtga tggtggtggt ggtcgtgcga atgggtgtgc gggtggctgt gcccgccgcc
     2821 gtggccgtgc tcgtcgggca ccagcagggt ggcgccgtgg cccgcgaact cctcgggcac
     2881 cagcagcgtc tgcccgccat aggtttgccg cagcatgtcc ggggtgagca cctcggcggg
     2941 cgggccgtag cagacgatgc cgtggttgac cagcacgatg cggtcgcacc actcggcggc
     3001 cagttcgagg tcgtgggtcg ccatgaccac ggtatgcccg gcggcgcgct gctgtcgcag
     3061 cagggtgccg atcacctcct gggtggaggc gtccacgccg ttcaacggct cgtccagcag
     3121 cagcaggtcg ccttcctgca ccagggcgcg cgccaggaaa acgcgctgct gctggccgcc
     3181 cgaaagctgg ctgatctgcc ggtgggcgaa gtcctgcatg ccgacctgga ccagcgcttc
     3241 gtgtgccagg cgctcatcgg cggggccggg ccggcgcccc cagcccaggc aggcggtgcg
     3301 gcccatcagc accacgtcgg ccaccgtgac cgggaagtcc cagttgacgg cctcgcgctg
     3361 gggcacgtag cccatggtgc ggcgagcggc gccgctcgcc accggctgcc cggccacacg
     3421 cacctcgccg ctgcgtgcgg gcaagatgcc caggatcgtc ttgatcagcg tcgatttgcc
     3481 cgcgccgttc ggcccgatgg cgcccacgat gaggccccaa tcgagcgtca ggttgacttt
     3541 ttccagcacc ggcggggcat cgtagcccac cgtcacatcg cgcagcacgg ccgcgggggc
     3601 ctgctgggcg gtgggccggg cagcggctgc cggggggtgc gcggtttgtc ctatcatttg
     3661 agtgccctca ctatggtctg cgtgttgtaa cgcatcatgc cctcgtacgt atacgccggg
     3721 ctgttgggcg gccccaacgt gtcgccgtag agcgtgtcca ccacgttcac accggcctcg
     3781 gcggcgatct gcttttcgag cttggggttg atcgagcttt ccaggaagat ggcgcgcacg
     3841 tgctcggcct tgatcttgtc tatcagctca gccagttgct tggccgacgg ttcggcgctg
     3901 ctgtccatgc tgggcacgac cgagccgacg aaggtcagcc catagcggcg gatgtagtag
     3961 ccgaaggcgt cgtggtttgt gaccatcttg cgctggtcgg gcgggatggt cgcgatctgg
     4021 ctcatgatat cgctgtcgag ttgcttcagc gtagcgaggt aggcatcggc gttggcctgg
     4081 taggcgtctt tgttggccgg atcggcggcg atcatggcgt cgcggatgtc gcgagtcatg
     4141 gtcatggcgt tggtcacgtc gaaccagaca tgcggatcgg ggccggactc ctcgccgtcg
     4201 ccggattcta gcttgacgcc cgtggtcacg gtgacgaccg ggtgattgcc cccggcgttc
     4261 tgcacgacct tatccaacca gtggtcgagg cccatgccgt ggcgcagcac caggtcggcg
     4321 ttggcgatca actggacatc gctgggcttg ggttcgtagg cgtggggatc gtcgctggcc
     4381 tgcaagatgg gcgtcacctt ggccgcgttg cccgcgacgg catcggccat cgagcggatt
     4441 tgcgtggtgg tgacggtgac gtggagcacg cgaccgccag cgggcggcgg gccgggggtg
     4501 ccgggcgtgg ccgagggggc cggcgagtcg ccgcacgcgc tcaacagcgc cgtgagcacg
     4561 gccagggcga cgatcaggcc gacacggcgg cctcgtttat gatacaacat tgcttcctgg
     4621 ttccttgtaa ccaatgg
//
LOCUS       DASUYC010000961         5478 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_2051898, whole genome shotgun sequence.
ACCESSION   DASUYC010000961 DASUYC010000000
VERSION     DASUYC010000961.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5478
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2051898"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1094
                     /locus_tag="VF276_15720"
     CDS             <1..1094
                     /locus_tag="VF276_15720"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX6608349.1"
                     /translation="MNRTGMNPNMVCDVVLGCANQAGEANRNVARMAALLAGLPITVG
                     GVTVNRLCGSGLDAINYAAANIRGNGGTAYVAGGVESMTRAPYVMAKPGEGFERGNRQ
                     MYDTTLGWRFTNPRLADMYYPYSMGETAENVAEKWHVSREEQDRFAYESQMKAKDAIE
                     GNCFRDEITPVPIPQKKGEPVPFTTDEFPRFDTTMEKLAKLRPAFREGGTVTAGNASG
                     INDGAAAVLVMAGRTVTELEYKPLARILSWAVAGVDPAYMGVGPVPATRKALKLAGLE
                     IADIDLIELNEAFASQSLVCIRELGLDPAKVNVNGGAIALGHPLGCSGARLMTTLVHE
                     MVRREVRYGLATMCIGVGQGIATIIERVP"
     gene            1305..2822
                     /locus_tag="VF276_15725"
     CDS             1305..2822
                     /locus_tag="VF276_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6608350.1"
                     /translation="MTQTATPATQPAPLERTDWKLFINGEQVDAASGATFETYNPATN
                     QVLAQVAQAGKEDVDRAVAAARAAFESGKWPRTSAARRGNLLIKVAEIMRKRSDEIAR
                     LEVMNNGKAISQAKAELTQAIEDFEFYAGAATKIMGQTIPVPGAFFNYTVREPIGVCA
                     QIVPWNYPIMMAAWKLAPALAAGNTVILKPASATPLTALLLGEICAEAGLPPGVVNVL
                     PGPGAEIGTYLAEHPGVDKIAFTGETTTGRDIMRRAAATLKRVTLELGGKSPNIVFED
                     ADLESAVNGSLWAIYYSAGQSCEARSRLFIHESLYDRFVEMFVAKAAKLKVGNPLDES
                     TQIGSLISRKQWNTVHSYVELGQREGAEVLVGGGRPEGDGLDQGNYYLPTALGNVDNK
                     MRVAQEEIFGPVVTMSRFSKEAEAVKLANDVVYGLAGTLWTKDVGRAIRVAGAIKSGV
                     VTINTPYTAFPGLPFGGYKQSGFGRELSMETLNLYTELKSVLIYTGERPINPFGL"
     gene            2929..3240
                     /locus_tag="VF276_15730"
     CDS             2929..3240
                     /locus_tag="VF276_15730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608351.1"
                     /translation="MNIRITKLYQDAPYSMLEIAFNSDPRGAEMIREIAEATGCTFER
                     PPGNRHALRELHLARIGDRTRLDEAYRTAGMVAEKYGPVHDSQKDMHGPDDRAADRGG
                     V"
     gene            3256..>5478
                     /locus_tag="VF276_15735"
     CDS             3256..>5478
                     /locus_tag="VF276_15735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608352.1"
                     /translation="MTVSPLAAAPGTTPGRSPARGPAPLVQRLTTPLALLLFLVVAAL
                     VTWPQLPHAADSLRDAGDPALFVWTLHWLATAVTHDPAQLYAGPIFHGFPNPVTFTDT
                     SLLPGVLLAPLWLADNHLLFLNLLMWVTLALVGFTAYLLIRRLSGSVLGGIVGGMVCA
                     VNGYTLAHLSHLNLLSAYWLPLGLLALHYLFHPDSRGPRWWVALALAAALIGQTLSTF
                     YVLAYFLIAMVAYVAWQALAIRAVWRWPGAWRWRLVAQLLVVALLVGAVIVAVTAPYW
                     ETNRVLGLARSAEEIATWSAQPLDYIGVSLHNHTYARLLPHSDPEPLFAGFTALILAA
                     IGAHGAWRQARRKAPPSAARVLFLDEAGFYAALTLLAALLTLGPSLDVGAWHIPLPYA
                     LLAQVPGATALRAPVRAMVLVNLGIAVFAGLGTVRVLAALTGRATGLTPPAPFPAREG
                     GDTGATTESGAADLPSPPRGGAGGEVAARWMRVLIVGALAVMIGVEQQVAPLALTPMP
                     ATAAELPPAYRWLTNHPDGGVLIEMPVGLGLRDPTVETTHMYYQTWHGHPLVSGYSGF
                     RPATYTEIFTFIDSQYADFTAEQLGLLQSLDVRYVLYHAANYKTSAWARVQAGLARFP
                     QVREVGHFPSGTYGDDYLYALDPRPASATLQITAMPGATANSLTVHITNPYTYPLLSR
                     LRPTLDLSTPDGRRIAVPTPLLLAPGEHRFAVALPGPSLAPPLALRPVVPFPSSLQGA
                     E"
BASE COUNT          882 a         1957 c         1819 g          820 t
ORIGIN      
        1 tgatgaaccg cacgggcatg aacccgaaca tggtctgcga cgtggtcctg ggttgcgcca
       61 accaggccgg cgaggctaac cgcaacgtgg cccgcatggc cgccctgctg gccggcctgc
      121 cgatcacagt gggcggggtc acggtgaacc ggctctgcgg gtcggggctg gacgcgatca
      181 actacgcggc ggccaacatc cggggcaacg ggggcacggc ctatgtggcc gggggcgtgg
      241 agagcatgac ccgcgccccc tacgtcatgg cgaagccggg cgagggcttc gagcgcggca
      301 accgccagat gtacgacacc acgctgggct ggcgcttcac caacccgcgc ctggccgata
      361 tgtattaccc ctacagcatg ggcgagaccg ccgaaaacgt ggccgagaaa tggcatgtca
      421 gccgcgagga acaggaccgc ttcgcctacg agagccagat gaaggccaag gacgccatcg
      481 aaggcaactg cttccgcgac gagatcacgc ccgtgccgat cccgcagaag aagggcgagc
      541 cggtgccgtt caccaccgac gagttcccgc gcttcgacac gaccatggag aaactggcga
      601 agctgcgccc ggcgttccgc gaaggcggca cggtcacggc gggcaacgcc agcggcatca
      661 acgacggggc cgccgccgtg ctggtcatgg ccgggcgcac cgtgaccgaa ctggagtata
      721 agccgctggc gcgcatcctc tcctgggccg tggcgggggt ggacccggcc tacatgggcg
      781 tcggcccggt cccggccacg cgcaaggcgc tcaagctggc gggcctggag atcgcagaca
      841 tcgacctgat cgagttgaac gaagccttcg cctcccagtc gctggtttgc atccgcgaac
      901 tgggcctgga cccggctaag gtcaacgtca acggcggggc gattgccctc ggccacccgc
      961 tcggctgctc gggcgcgcgc ctgatgacca cgctggtgca cgagatggtg cgccgcgagg
     1021 tgcgctacgg cctggcgacg atgtgcatcg gcgtgggcca gggcatcgcc accatcatcg
     1081 agcgcgtccc gtaaacagca caggccggct attcagcggc tacggccctc ttgcgatgta
     1141 ggggccggtt tcaaaccggc ccgcgcatca gacaacagga cacgcactct gttcaccggc
     1201 taccgccatt tcgcgttgta ggggccggtt tcaaaccggc ccgccgcgcc ggagaatcgt
     1261 ccgaagcggc gaccaatcga ttcacacaca aaggagtgtt acgtatgacc caaaccgcaa
     1321 cgccggcaac gcagccggcc ccgttggagc gcaccgactg gaagttgttc atcaacggcg
     1381 agcaggtcga tgcggcgagc ggcgccacgt tcgagaccta caaccccgcc accaaccagg
     1441 tgctggcaca ggtcgcccag gccgggaagg aagatgtgga ccgtgcggtg gcggcggcgc
     1501 gcgccgcgtt cgagagcggc aagtggccgc gcacctcggc agcccgccgc ggcaacctgc
     1561 tgatcaaagt cgccgagatc atgcgcaagc gcagcgacga gattgcccgg ctcgaagtga
     1621 tgaacaacgg caaggcgatc agccaggcca aggccgagct cactcaggcc atcgaggact
     1681 tcgagttcta cgccggggcg gccaccaaga tcatgggcca gaccatcccg gtgccgggcg
     1741 ccttcttcaa ttacacggtg cgcgagccca tcggcgtgtg cgcccagatc gtgccctgga
     1801 attacccgat catgatggcg gcctggaagc tggcccccgc gctggccgcg ggcaatacgg
     1861 tgatcctcaa gcccgccagc gccacaccgc tgaccgcgct gctgctgggc gagatctgcg
     1921 ccgaggccgg gctgccgccg ggcgtggtca acgtgctgcc ggggccgggc gccgagatcg
     1981 gcacctacct ggccgagcac cccggcgtgg ataagatcgc cttcaccggc gagacgacca
     2041 cgggccgcga catcatgcgc cgcgccgccg ccacgctcaa gcgcgtgacg ctggaactcg
     2101 gcggcaagtc gccgaacatc gtgttcgagg acgccgatct ggagagcgcg gtcaacggct
     2161 cgttgtgggc catctactac agcgccgggc agtcgtgcga ggcgcgttcg cgcctgttca
     2221 tccacgagag cctctatgat cgctttgtcg agatgttcgt cgccaaagcg gcgaagctga
     2281 aggtcggcaa cccgctggac gagtcgacgc agatcggctc gcttatctca cgcaagcagt
     2341 ggaacaccgt ccacagttat gtcgagctag gccagcgtga aggggccgag gtgctggtcg
     2401 gcggaggccg cccggaaggc gacggcctgg atcagggcaa ctactacctg cccacggcgc
     2461 tcggcaacgt cgataacaag atgcgcgtgg cccaggaaga gatcttcggc ccggtcgtca
     2521 ccatgtcccg cttcagcaag gaagccgagg cggtcaaact ggcgaacgac gtggtctacg
     2581 gcctggcggg cacgctctgg accaaagacg tgggccgcgc catccgcgtc gcgggcgcca
     2641 tcaagtcggg cgtcgtgacg atcaacacgc cctacacggc gtttcccggc ctgcccttcg
     2701 gcggctacaa gcagtcgggc tttggccgcg aactgagcat ggaaacgctc aacctgtaca
     2761 ccgaactgaa gagcgttttg atttacaccg gcgaacggcc catcaatccg ttcggcctct
     2821 agccgggcgc ctcgcgcccg tggcgctgtt cgccacgggc gcgataaatc gcgccgctac
     2881 gaatcaccat acgggcgcga tgcatcgcgc cactacgaat tgctgctaat gaatatccgc
     2941 atcacgaaac tttaccagga cgcgccgtac agcatgctgg agatcgcctt caacagcgac
     3001 ccgcgcggcg ccgagatgat ccgcgagatc gccgaggcga cgggctgcac gtttgagcgc
     3061 ccgcccggca accgccacgc cctgcgcgag ctgcacctgg cgcgcatcgg cgaccggacc
     3121 cggctggacg aagcctaccg cacggcgggg atggtggccg agaagtacgg cccggtccac
     3181 gacagccaga aggacatgca cggccccgac gaccgcgccg ccgaccgggg cggggtctga
     3241 tctgccgcgc cccgcatgac cgtctcaccg ctcgccgcgg cgccagggac cacgccgggc
     3301 cggtcgcccg cacgggggcc cgcgccgctc gtgcagcgcc tcaccacgcc cctggcgctg
     3361 ctgctgttcc tggtcgtcgc cgccctggtg acatggccgc aactgccgca cgccgccgat
     3421 tcgctgcgcg atgccggcga cccggcgctg ttcgtctgga cgctgcattg gctcgccacc
     3481 gccgtcacgc acgatcccgc gcagctctac gccgggccga tcttccacgg cttccccaat
     3541 cccgtcacct ttaccgacac cagcctgctg ccgggcgtgc tgctggcccc gctctggctg
     3601 gccgataatc acctgctgtt cctgaacctg ctgatgtggg tgacgctggc cctggtcggc
     3661 ttcaccgcct acctgctgat ccggcgcctg agcggcagcg tcctcggcgg gatcgtcggc
     3721 gggatggtct gcgcggtgaa cggttacacg ctggcccacc tcagccacct gaatctgctc
     3781 tcggcctact ggctgccgct ggggctgctg gcgctgcatt acctcttcca ccccgattcg
     3841 cgtggcccgc gctggtgggt ggcgctggcg ctggccgcgg ccctgatcgg gcagacgctg
     3901 agcacgttct atgtgctggc ctacttcctg atcgccatgg tggcctatgt cgcatggcag
     3961 gcgctggcga tccgcgccgt ctggcgctgg ccgggcgcgt ggcgatggcg gctggtcgcg
     4021 caactgctgg tggtagcgct gctggtaggc gcggtcatcg tcgcggtcac ggcgccctac
     4081 tgggagacca accgcgtgct gggcctggcc cgcagcgcgg aggagatcgc gacctggtca
     4141 gcacagccgc tcgattacat cggcgtatcg ctgcacaacc acacctacgc ccggttgctg
     4201 ccgcatagcg atcccgaacc gcttttcgcc gggttcaccg cgctgatcct ggccgccatc
     4261 ggggcgcacg gcgcgtggcg gcaggcccgg cgcaaagccc cgccgtccgc tgcgcgcgtg
     4321 ctgttcctgg acgaggcggg cttctacgcc gccctgaccc tgctcgccgc gctgctcacc
     4381 ctcggcccca gcctggatgt gggcgcctgg cacatcccgc tgccctacgc gctgctggcc
     4441 caggtgcccg gcgctacggc gttgcgcgcc ccggtgcggg ccatggtact ggtcaacctg
     4501 ggcatcgccg tcttcgcggg cctgggcacc gtgcgcgtcc tggccgcgct gaccgggcgg
     4561 gccaccggcc taaccccccc ggcccccttc ccggcgcggg aagggggaga tacgggcgct
     4621 accacggaat ccggcgccgc cgatctcccc tccccgccgc ggggaggggc cgggggagag
     4681 gttgccgccc ggtggatgcg cgtgctgatc gtgggggcgc tggcggtgat gatcggggtc
     4741 gagcagcagg tggcgccgct ggcgctaacg cccatgccgg ccaccgccgc cgagttgccg
     4801 cccgcctacc gctggctcac gaaccacccg gacggcggcg tgctgatcga gatgccggtg
     4861 ggcctgggct tgcgcgatcc gaccgtcgag accacgcaca tgtactacca gacgtggcac
     4921 ggccacccgc tggtcagcgg ctacagcggc ttccgcccgg ccacgtacac cgagatcttc
     4981 acgttcatcg actcgcagta cgccgatttc accgccgagc aactgggcct gctgcaaagc
     5041 ctggacgtgc gctatgtcct ctaccacgcg gccaactaca agaccagcgc ctgggcgcgg
     5101 gtgcaggccg ggctggcccg gttcccgcaa gtccgcgagg tagggcactt cccgtccggc
     5161 acctacggcg acgactacct gtacgcgctc gacccccgcc cggcgtccgc caccttgcag
     5221 atcacggcta tgcccggtgc gacggccaat agcctcaccg tgcatatcac caacccctac
     5281 acctatccac tgctcagccg cctgcggccc acgctcgatt tgagcacccc cgatgggagg
     5341 cgcatcgccg tccctacgcc gctgctgctc gcccccggcg aacaccgttt cgcggtggcg
     5401 ctgcccggcc catcgctcgc gccgccctta gccctgcggc cggtagtgcc cttcccgtcg
     5461 agcctgcaag gggccgaa
//
LOCUS       DASUYC010000962         3370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_621753,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000962 DASUYC010000000
VERSION     DASUYC010000962.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621753"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /locus_tag="VF276_15740"
     CDS             complement(<1..1070)
                     /locus_tag="VF276_15740"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6608353.1"
                     /translation="MAKREFSVADEYPYDRRSPVRWVLSHGLRYKRFLIGMIVAIAAS
                     NVMDAAIPALTGLAFNEVLRPQPSRDQLLLIALAILAAVIGRGVVNAAGHFANEFLGK
                     RMQRDARDELYVNLLGKSQTFHNRQRVGDIMARASNDASQLSDMVVPGFDILFDSFSS
                     LAITLVFIAGINPQLLLAPVLFTIAFLIALRDYMRRLRPVADQMRAQFGTVNAGLAEA
                     VTGIEVVKATAQEEQERRKFEHNARAFRDYFVQNGRIQARYLPTLLVAIAMAGGFLHG
                     VFLLSRGELSLGGLIAYMGLLGLMRFPAGISVWTFNVVQNGVAAAGRFLALLKEETDL
                     DENEGGHRAPIRGEIVFDHVTF"
     gene            complement(1072..1302)
                     /locus_tag="VF276_15745"
     CDS             complement(1072..1302)
                     /locus_tag="VF276_15745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608354.1"
                     /translation="MYYGVHTPNFGDYGDATLPIPLVHEAVGPGSPGITSGLRPGCPP
                     LTWPWGALNGKAPRGGHMHNQSVSRRFVKGRS"
     gene            2179..>3370
                     /locus_tag="VF276_15750"
     CDS             2179..>3370
                     /locus_tag="VF276_15750"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6608355.1"
                     /translation="MRILVTEPLDDAGLILLRQQATVDVRLNLPREELLRRAGDYEAI
                     ITRSGTPVDRALLDAGTALRVIGRAGIGVDNIDIPAATERGIAVVNAPNGNVRAAAEH
                     TIGMIFALARNIPQADHLLRQGVWGKNKFMGTEISGKTVGIIGLGKVGTQVARRLGAF
                     DMEVLAYDPFRAQEAEGVTFTTLDDLLARADIVTLHVPLTAITRNMIGERELALMKPG
                     AFLINVARGKVVDEAALVAACRAGHLGGAALDVFAKEPFDNPALLDLHNVILTPHLGG
                     TTHESMRASAVEVAEEVLAVLAGRTPQNICNPEVLTRGLWPAPAPRLLNGWLGFQTVV
                     LDCDSTLTQIEGVDELAAMRGCREEVAALTQQAMAGELPMEAVFESRLERIQPTQADM
                     ARLGE"
BASE COUNT          586 a         1087 c         1153 g          544 t
ORIGIN      
        1 ctaaaggtga cgtggtcgaa cacgatctcg ccgcggatcg gggcgcggtg gccgccctcg
       61 ttctcgtcca ggtcggtctc ctctttgagc agagccagga agcgccccgc cgccgccacg
      121 ccgttctgca ccacgttgaa ggtccagacg gagatgcctg ccgggaagcg catgaggccg
      181 agcaggccca tgtaggcgat cagcccgccc aggctcaact cgccgcgcga cagcaggaac
      241 acgccgtgca ggaagccccc ggccatggcg atggcgacca gcagggtcgg caggtagcgc
      301 gcctggatgc gcccgttctg gacgaagtag tcgcggaagg cccgcgcgtt gtgctcgaac
      361 ttgcgccgct cctgttcctc ctgcgcggtg gccttgacca cctcgatgcc ggtcacggct
      421 tcggccagcc cggcgttcac cgtgccgaac tgggcgcgca tctggtcggc caccgggcgc
      481 aggcggcgca tgtagtcacg cagggcgatc aggaaggcga tggtgaacag caccggcgcc
      541 agcaacagct gcgggttgat cccggcgatg aagaccagcg tgatggcgag gctggagaac
      601 gagtcgaaca ggatgtcgaa ccccggcacg accatgtcgc tcaactggct cgcgtcgttg
      661 gaggcgcggg ccatgatgtc gcccacgcgc tggcggttgt ggaaggtctg gctcttgccc
      721 agcaggttga cgtagagttc gtcgcgggcg tcgcgctgca tgcgcttgcc caggaactcg
      781 ttggcgaagt ggcccgcggc gttgaccacc ccgcgcccga tcacggcggc caggatggcg
      841 agcgcgatca gcagcaactg gtcgcggctg ggctgcgggc gcaacacctc gttgaaggcc
      901 agcccggtga gcgccgggat cgccgcgtcc atcacgtttg aggcggcgat ggcgacgatc
      961 ataccgatca ggaagcgctt gtagcgcagc ccatgcgaca gcacccagcg caccggcgag
     1021 cgccggtcgt agggatactc atcggccacg ctaaactctc gtttcgccac gctaactcct
     1081 ccctttaacg aaccggcggc tcaccgattg gttgtgcatg tgcccgccgc gcggggcctt
     1141 gccgttcagc gcgccccagg gccaggtgag cgggggacag ccggggcgaa gacccgatgt
     1201 aattcccggt gagccggggc cgacggcctc gtgaaccagc gggatcggta acgtggcgtc
     1261 gccatagtcg ccaaagttcg gagtgtggac accatagtac aagcggcgct cctttccgcg
     1321 gcccgcgacg gcccgcgctg aacagggtgc tgtccggtgg tggtgtgtgc tatgtatccg
     1381 cggcagtaga acaaacgtgc aaaccagtgt agcagacggg accgggctga gtcaatcagc
     1441 cgacagaccg agtttcgccc gcccgatggt ccgatgggcc aggtaacggc ggggcgagtc
     1501 ttaacacgct ctcccagcgt gtttgtcact ctgagcgccg cgaagagtct cgtctttggg
     1561 gccacgagac ggagattctt cgctccgctc agaatgacgg gtctcccggc agtgtcactc
     1621 tgagcgccgc gaagagtctc gtcgtttggg ctacgagacg gagatttttg ctccgctcag
     1681 aatgacactg ctcatatgat ggactaggta gccgcgataa tcgaatattc cgacaaaatc
     1741 gtaagcctgg ttttggacaa atgtcccgcc cgcggacctt gacgggcacg gcatttgcat
     1801 atataatgcg gacaagtggc agcgctgccg ccgttgtgcg aagcgctgcc tgtatttttc
     1861 tatggagctc ggccatgatt ccattgtccc tctggtcgca gtcgaatagc gcgagtatgg
     1921 gcccttgtgg ttgccggtac agtgccggtg gcagccacaa gggcggttct ggcttgcgcg
     1981 agagtgccgc gccgggggga cggaaggcat ccgtcggccc tgacacacaa ctaggaggat
     2041 gcgctatcca gccgtaagcg gctgcgtgga taaccgcata ccgatcctgg ccccgcactc
     2101 caagcgagtg gggggttttt gtttgccggg taggccggtc cgggtgccgg cgatgagcga
     2161 cataggagat acgagaccat gcgaattctg gtgaccgaac cgttagatga cgccggcctg
     2221 attttgctgc ggcagcaggc caccgtggat gtgcgcctga acctgccgcg ggaagagttg
     2281 ttgcgccgcg cgggcgacta cgaagccatc atcacgcgca gcggcacgcc ggtggaccgc
     2341 gcgctgctcg acgccgggac cgcgctgcgg gtgatcgggc gggcgggcat cggcgtggac
     2401 aacatcgaca tccccgccgc caccgagcgc ggcatcgccg tggtgaacgc gcccaacggc
     2461 aacgtgcgcg ccgccgccga gcataccatc ggcatgatct tcgcgctggc ccgcaacatc
     2521 ccgcaggccg accatctgtt gcggcagggg gtgtggggca agaacaagtt catgggcacc
     2581 gagatcagcg gcaagacggt gggcatcatc gggctgggca aggtcggcac gcaggtggcg
     2641 cgccgcctgg gcgccttcga tatggaggtg ctggcttacg acccctttcg cgcccaggaa
     2701 gccgagggcg tcacgttcac cacactggac gacctgctgg cccgcgccga catcgtcacc
     2761 ctgcatgtgc cgctgaccgc catcacgcgc aacatgatcg gcgagcgcga actggcgctg
     2821 atgaagcccg gcgccttcct gatcaacgtg gcgcgcggca aggtggtgga cgaggcggcc
     2881 ctggtcgcgg cctgccgtgc cgggcacctg ggcggggccg cgctcgacgt gttcgccaaa
     2941 gagccgtttg acaaccccgc cctgctcgac ctgcacaacg tcatcctcac cccgcacctg
     3001 ggcggcacca cgcacgagtc gatgcgcgcc agcgccgtcg aagtggccga agaagtgctg
     3061 gccgtcctgg ccgggcgcac cccgcagaac atctgcaacc ccgaagtgct gacccgcggc
     3121 ctctggcccg ccccggcgcc caggctgctc aacggctggc tcggcttcca gacggtggta
     3181 ctcgactgcg acagcaccct gacgcagatc gagggcgtgg acgaactggc ggccatgcgc
     3241 ggctgccgcg aggaagtagc cgccctgacc cagcaggcca tggccgggga actgccgatg
     3301 gaagcggtgt tcgagtcgcg cctggagcgc atccagccca cgcaggccga catggcgcgc
     3361 ctgggcgaac
//
LOCUS       DASUYC010000963         3145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1313953, whole genome shotgun sequence.
ACCESSION   DASUYC010000963 DASUYC010000000
VERSION     DASUYC010000963.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1313953"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="VF276_15755"
     CDS             <1..587
                     /locus_tag="VF276_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HEX6608356.1"
                     /translation="ALLAGLPVRLAALADVGITDDVEEGDESFVANARLKAETYWRMA
                     RERGLRAWVLADDSGLEVDALGGEPGVRSTRWAGPNTTAAERNTRLLARLTEVPMEER
                     GARFRCLIVLLSPDGTEYVGDGTLEGRITFAPRQHPGYGFGYDPIFELPERGLTVGEI
                     PPAEKEAISHRGRAGRQIRTVLAQALAEPPAAPA"
     gene            complement(597..1091)
                     /locus_tag="VF276_15760"
     CDS             complement(597..1091)
                     /locus_tag="VF276_15760"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX6608357.1"
                     /translation="MMESQDAYIEGQRLERGPDAPDGENWGYSFPIVVRFNDLDAMGH
                     VNNAVYLTYCEMARVGYLRTVGGMGAFGQLDMILARAEVDYRLPIEFTDRLAVQVRAT
                     AIGTKSFTLDYRMLIERTGRRHVACTARSVIVCYDYTAGHSVPVSPALIDCFETFEGR
                     RLRR"
     gene            complement(1217..1372)
                     /locus_tag="VF276_15765"
     CDS             complement(1217..1372)
                     /locus_tag="VF276_15765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608358.1"
                     /translation="MNSRLIIGIVAILLGLIVLVWPAFLSVIVGLCLIGFGLWFTLQG
                     AGQGTGV"
     gene            complement(1413..2708)
                     /locus_tag="VF276_15770"
     CDS             complement(1413..2708)
                     /locus_tag="VF276_15770"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX6608359.1"
                     /translation="MERRCSELIDKPIITYDTGRRLAHVEDLLVDPERNQVLALLIDK
                     GAVFVSPKVVPFGHIKSIGNNAIVVPKPDVIYNVSRIPELKRVFDGRTIRGMRVYGEN
                     GDRLGTIGDMIIDDRTGEILYYEVSGGAIGDAMKGKRTIVPSEILNMGALVLYVSAAT
                     AARLEQQQGGVTGAFEQARQRVNQFSDQAADATTARVSRLTDQAKQQQRSYIIGRTAL
                     RTVTGPDNQPIVREGEIITEDTVAQAEQQGRLPALLMAGGMGEAQQHLGNLGQQANDS
                     FAQIREEATHLWNQLTHHTDRITGETESKIEQQRIDRALGRPASRVILDRQDNVILNT
                     GDIITNAAVEQARAAGVLNILLDSVYTQAPKLSLEDLKAPWSGRASLEETQNEPDTPG
                     VPRVPMAPAAPAPLARSTAANAAAQPTAPNPASTSTRTR"
     gene            complement(2739..2894)
                     /locus_tag="VF276_15775"
     CDS             complement(2739..2894)
                     /locus_tag="VF276_15775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608360.1"
                     /translation="MARFVGKETDVGAQFLSRILTFILLLVVLLILLKLWHPAFAQVI
                     LSPLGLW"
BASE COUNT          512 a         1074 c         1058 g          501 t
ORIGIN      
        1 aggcgctgtt ggccggattg ccggtgcgcc tggcggcgct ggccgacgtg ggcatcaccg
       61 acgatgtgga ggaaggcgac gagagtttcg tcgcgaacgc acggctgaag gcggagacct
      121 actggcggat ggcgcgcgag cgcgggctgc gcgcctgggt gctggccgac gacagcgggc
      181 tggaggtgga tgcgctgggc ggcgagccgg gcgtgcgcag cacgcgctgg gccggcccca
      241 acaccaccgc cgccgaacgc aacacgcgcc tgctggcccg gctcaccgag gtacccatgg
      301 aggagcgagg cgcccgcttc cgctgcctga tcgtgctgct gagtcccgac ggcaccgagt
      361 atgtcggcga cggcacgctg gaagggcgca tcacctttgc gccgcgccag caccccggct
      421 acggcttcgg ctacgacccg atcttcgagc tacccgagcg cggcctgacc gtcggcgaga
      481 tcccgcccgc cgagaaagag gcgatcagcc accggggccg cgccgggcgc caaatccgca
      541 ccgtgctggc ccaggcgctc gccgaaccgc ccgccgcgcc ggcttaacac cggcccctag
      601 cgacgcaggc gccgcccctc gaacgtctcg aagcagtcga tgagcgccgg ggacacgggg
      661 acgctgtgcc cggcggtgta gtcgtagcag acgatgaccg agcgcgccgt gcaggccacg
      721 tggcgccggc cggtgcgctc aatcagcatc cggtagtcca gggtgaagct cttggtgccg
      781 atggccgtgg cccgcacctg caccgccagc cggtcggtga actcgatggg caggcggtaa
      841 tccacctcgg cgcgggccag gatcatgtcc aactgcccga aggcgcccat cccgcccacg
      901 gtacgcaggt agcccacccg cgccatctcg caataggtga gatacaccgc gttgttgacg
      961 tggcccatcg catcgaggtc gttgaagcgc acgacaatgg ggaagctata gccccagttc
     1021 tctccgtctg gcgcatccgg cccgcgctcc agccgctgcc cctcgatata cgcatcttgc
     1081 gattccatca tagcctggat gataccggct tgcgcgaaac catgtcaagg cgccacagtg
     1141 atcggttgct ccagaacgcg caaaagggga ggcgcccccc ggcaacctcc cctgcgtgcc
     1201 atgcccggca gcccggctag acgccggtgc cctgtccggc cccctgcaag gtgaaccaga
     1261 ggccgaaccc gatcaggcac aggcccacga tgaccgacag gaaagccggc cagacgagca
     1321 caatcaggcc cagcagaatg gcgacgatgc cgatgatcag acgactgttc atgtagcaaa
     1381 ctccttgttg gcgcgaccgc gccgcgccgg tgttagcgag tgcgggtgga tgtggacgcc
     1441 ggattcggcg ccgtgggctg cgcggcggca ttcgccgccg tggaccgggc cagcggggcc
     1501 ggcgccgcgg gcgccatcgg cacgcgcggc acacccggcg tgtccggttc gttctgtgtc
     1561 tcttcgaggc tggcgcgccc gctccagggc gctttcaggt cctccaggct cagcttcggc
     1621 gcctgggtat agaccgagtc gagcaagata ttcagcacgc cggccgcgcg ggcctgttcc
     1681 accgcggcat tggtgataat atcgccggtg ttcaggatca cattgtcctg ccggtccagg
     1741 atcacccgcg aggccgggcg gccgagcgcg cggtcaatgc gctgctgctc gatcttgctc
     1801 tcggtctcgc cggtgatgcg gtcggtatgg tgggtaagct ggttccagag gtgcgtggcc
     1861 tcctcgcgga tctgggcaaa gctgtcgttg gcctgctggc cgagattgcc caggtgctgc
     1921 tgggcctcgc ccatgccgcc cgccatcagc agcgcgggca gacgcccctg ctgctcggcc
     1981 tgggccaccg tgtcctcggt aatgatctcc ccttcgcgca cgatgggctg gttgtccggg
     2041 ccggtgaccg tgcgcaatgc cgtgcgcccg atgatatacg agcgctgctg ctgcttggcc
     2101 tggtcggtca gccgggaaac ccgcgccgtg gtcgcgtcgg cggcctggtc cgagaactgg
     2161 ttgacgcgct ggcgggcctg ctcgaacgcg ccggtgacgc cgccctgctg ctgctccagg
     2221 cgcgccgccg tcgccgccga gacgtagagc accagcgcgc ccatgttgag gatctcgctc
     2281 ggcactatgg tgcgcttgcc cttcatggca tcgccgatgg ccccgcccga gacttcgtag
     2341 tagagaatct cgccggtgcg gtcgtcgatg atcatgtcgc cgatggtgcc caggcggtcg
     2401 ccgttctcgc catagacgcg catcccgcgg atggtgcgcc cgtcgaacac gcgcttcaac
     2461 tcgggaatac gcgagacgtt gtagataaca tcgggcttgg gcaccacgat ggcgttgttg
     2521 ccgatggact tgatgtggcc gaagggcacc accttcggcg agacgaacac ggcgcccttg
     2581 tcgatcaaca gcgccagcac ctggttgcgc tccgggtcca ccagcagatc ttcgacatgg
     2641 gccaggcgcc gcccggtgtc gtaggtgata atcggcttgt caatcaattc gctgcaacgg
     2701 cgttccatcc tgcacctcca tcctctctca gtaccggctt accacagccc cagcgggctg
     2761 aggatgactt gggcgaaggc tgggtgccac agcttcaaca ggatcaacag gacgaccagc
     2821 aacaggataa aggtgaggat gcggctcagg aactgggctc ccacatccgt ctctttccca
     2881 acaaaacgcg ccatagcact actccttact tggacccgca gcgggccact ccaggcgggc
     2941 atcccgtcca cgctccggta cgcgacgccc cggcgcgcat tgcaacgcac cctgcaagac
     3001 cggacacata tgcgtgacct taccgccggg aataagcaag cgacgtgcca gccgtgccgg
     3061 gccgttagca gcccacggcg gccaggatgg cggtgatgag gtcgccgggc gtgcggtcgt
     3121 cggcggcgat ctgccgggcg aagag
//
LOCUS       DASUYC010000964         2977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_391388,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000964 DASUYC010000000
VERSION     DASUYC010000964.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391388"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VF276_15780"
     CDS             <1..395
                     /locus_tag="VF276_15780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608361.1"
                     /translation="RIIVALVLIAHGIGHSMGVLGAWTTIPSGLTNRSWLFSSGVLVD
                     SATGRVWSLLWLAALVVTTAGGLGLLLQQDWWRTVAVAGAILSLVAIVPWLAAMPLGS
                     AIGAVAIDILVLAILLLPVGEQFVRQLR"
     gene            743..1198
                     /locus_tag="VF276_15785"
     CDS             743..1198
                     /locus_tag="VF276_15785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608362.1"
                     /translation="MNRTVMYLAVAAFVVVALLTPFELFGVPLAPLAAIVFGAGAGWW
                     VAATRPEHTAGNAARAGAWVGLGALLGSVIGLAVLALWIGNIPEVQAFVQSSEPHPEA
                     RIPIDWIAPLGALAGVIAGFFAGVVDLVLSWIAGWIAGMVTTRNVTAHV"
     gene            1251..1595
                     /locus_tag="VF276_15790"
     CDS             1251..1595
                     /locus_tag="VF276_15790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608363.1"
                     /translation="MQTLLPPIREPPVAPLDDGPDPEEPRPAPFAFLDPAPVTGGRLW
                     GVIVISALFTGVLIALESGTSPWIVSLGRAIIWLALTALAVLGMTIWESEDRVPVKVA
                     IGGFMLLMWFGL"
     gene            1723..2706
                     /locus_tag="VF276_15795"
     CDS             1723..2706
                     /locus_tag="VF276_15795"
                     /inference="COORDINATES: protein motif:HMM:NF042511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6544 family protein"
                     /protein_id="HEX6608364.1"
                     /translation="MNMLTHPAQNSDAAAPASAGPRRRIPRVVLGAGAGLLLARLVRA
                     RLLARPAPFAAVAPPFVPPETQPLPADLPAPVARFYRLRYGDAVPVIHTAVLSGRGVM
                     RLGGIPIPVRWRFLHEAERNFRAYFELTLAGIPVLRANEHYRDGHFRQELPFGVEQGE
                     PKNDHSAALRMWAEWALWLPALLLADPAVRWLPVDDETALLAVPVGTTQEHLVVRFDA
                     ATGDLQYVEAMKYKHATDIRKTLWANAVWFGDRPWADFQREDTVYNVPIDTTLDARGP
                     VPAPGVPAPVALPIEGTAAPAERGEESPLVTVSGAALPPAPPPDFAGANGA"
     gene            2735..>2977
                     /locus_tag="VF276_15800"
     CDS             2735..>2977
                     /locus_tag="VF276_15800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608365.1"
                     /translation="MITTQAPSATRDRVLWADVGLAGAAALAYLLIGAQVLAVGDLQP
                     ADAPPAIIYVCAGCYLLGGLLILLQRRALWIAGAVIN"
BASE COUNT          379 a         1055 c         1070 g          473 t
ORIGIN      
        1 tccgcatcat tgtcgcgctg gtgttgatcg cccatggcat cggccacagc atgggcgtgc
       61 tgggcgcctg gaccaccatt ccgtccgggc tgaccaaccg gtcctggctg ttctccagcg
      121 gcgtgctggt ggacagcgcc acgggccgcg tctggagcct gctctggctg gccgcgctgg
      181 tcgtaaccac ggcgggcggg ttgggcctgc tgctgcaaca ggactggtgg cgcacggtgg
      241 ccgtggccgg ggccatcctg tcgctcgtcg ccatcgtgcc ctggctggcg gccatgccgc
      301 tcggctcggc catcggcgcc gtcgccatcg atattctcgt gctcgccatc ctgttactgc
      361 cggtgggcga acagttcgtc cgccaactgc ggtaagcgcc taacgagtgg tcagtggtca
      421 gtggtcagtg ggcagtggtc ggtgggcagc cgtgagccgt gagccgtcag cggtcaggag
      481 acgggtgacg agtgggcagt gggccgtggt cggtggcagc cgtgagccgt cagccgtcag
      541 cgatcaggag acgggtgacg agtgggcagt gggccgtggg cagctatgtg ctacgcaccg
      601 ctgcgcgaac aggagacggg tgatcagggg gcagggggca gcattgagtt gagtatggat
      661 tgtcattctg agcggagcga agaatctcgg ctttgcggcg ggccgcggca ccggttgcgg
      721 cgcgccggga aaggaacgtg agatgaaccg cactgtcatg tatctggccg tggcggcctt
      781 tgtcgtcgtg gcgctgctca cgccgttcga actgttcggc gtgccgctgg ccccgctggc
      841 ggccatcgtg ttcggcgccg gcgcggggtg gtgggtggcg gccacccgcc cggagcacac
      901 cgccggcaac gcggcgcgcg ccggggcctg ggtggggctg ggcgccctgc tcggctcggt
      961 catcggcctg gccgtgctgg ccctctggat cggcaatatc cctgaggtgc aagccttcgt
     1021 ccagagcagc gaaccgcatc ccgaagcccg gatccccatc gactggatcg cgcccctggg
     1081 cgcgctggcc ggggtgatcg ccggcttctt cgccggggtc gtggatctgg tcctgtcgtg
     1141 gatcgccggg tggatcgccg ggatggtcac cacccgcaac gtcacggcgc acgtctagcg
     1201 gcacaggggg cgcggaccgg cgccgcaacg ccgcgccagg agggtctact atgcaaacgc
     1261 tgctcccgcc cattcgagaa ccgcccgtcg ccccgctgga cgatggcccg gacccggagg
     1321 aaccacgccc cgcccccttc gccttcctcg accccgcgcc cgtcaccggc gggcggctgt
     1381 ggggcgtgat cgtcatctcc gcgctgttca caggggtgct cattgcgctg gagtccggca
     1441 cgtcgccctg gatcgtgtcg ttaggtcggg ccatcatctg gctggccctg accgcgctgg
     1501 ccgtactggg gatgaccatc tgggagtcgg aggaccgggt accggtaaag gtggcgattg
     1561 ggggattcat gctcctgatg tggttcgggc tgtaatcgct ccagcccggc gctctacccg
     1621 gccccgtcgt aggccggagc attggacaat acgtgccgcc ggcgcgccgg ccgccctgga
     1681 cgggaaggtc tggccgccgc cgcacaaggg agaactaaac cgatgaacat gctgacccat
     1741 cccgcacaaa atagcgacgc ggccgccccg gcctccgctg gcccccgccg ccggattccg
     1801 cgcgtggtcc tgggggcggg cgccgggctg ctgctggcgc ggctggtgcg cgcccggctg
     1861 ctggcccggc ccgcgccctt cgccgccgtg gccccgccct tcgtgccgcc ggagacccaa
     1921 ccgctgcccg ccgatctgcc cgcgccggtg gcccgcttct atcgcctgcg ctacggcgac
     1981 gccgtgccgg tgatccacac cgcggtgctc agcgggcggg gcgtcatgcg cctgggcggc
     2041 atccccatcc ccgtgcgctg gcgcttcctg cacgaagccg agcgcaactt ccgcgcctac
     2101 tttgagctga cgctcgccgg catcccggtg ctgcgggcca acgagcacta ccgcgacggc
     2161 cacttccgcc aggagctacc cttcggcgtg gagcaaggcg agccgaagaa cgaccactcg
     2221 gcggcgctgc ggatgtgggc cgagtgggcg ctctggctgc ccgccctgct gctggccgac
     2281 ccggcagtgc gctggctgcc ggtggacgac gaaaccgcgc tgctggccgt gcctgtcggg
     2341 accacgcagg agcacctggt ggtccgcttc gacgccgcca ccggcgacct gcaatatgtc
     2401 gaggcgatga agtacaagca cgccaccgac atccgcaaga cgctctgggc caacgccgtc
     2461 tggtttggcg accggccctg ggccgacttc cagcgcgaag acaccgtcta caacgtgccg
     2521 atcgacacca cgctcgatgc ccgcggcccg gtgcccgcgc ccggcgtccc cgccccggtg
     2581 gccctgccta tcgagggcac cgccgccccc gcggagcgcg gcgaagagtc gccgctcgtc
     2641 acggtcagtg gcgcggccct gccgccggcg cccccgccgg acttcgccgg tgccaacggg
     2701 gcgtaggccc cgaccgcccg caggaggtgc caccatgatc accacgcaag ccccgtccgc
     2761 cacgcgcgac cgcgtgctat gggccgacgt gggcctggcc ggggcggcgg ccctggccta
     2821 tctgctcatc ggcgcgcagg tgctcgccgt gggcgacttg cagccggccg acgcgccgcc
     2881 cgccatcatc tacgtctgcg cgggctgcta tctgctcggc gggttgctga tcctgctgca
     2941 gcggcgtgcc ctgtggatcg cgggcgccgt gatcaac
//
LOCUS       DASUYC010000965         2984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1270252, whole genome shotgun sequence.
ACCESSION   DASUYC010000965 DASUYC010000000
VERSION     DASUYC010000965.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270252"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            337..1107
                     /locus_tag="VF276_15805"
     CDS             337..1107
                     /locus_tag="VF276_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015888633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEX6608366.1"
                     /translation="MNNKTILGSTAAVALLGLGMLAGSVVGTSQAFAQTGPATASPTA
                     VVTTPAAPATTPSSGPSTVPVAPGTGQAPSAAPAPAITQQQAEQAALAARPGNTVDHT
                     HLMNDNGQAFWDVDFSNGGGVKIDAQTGAVIAVEAAGTDQGGPHGDHGADQAALAAQA
                     TVSQADAEKAALAASPGNTIDHSRLGDDNGTVYWDVDFTNGGGVEVNAQTGAIIAVEA
                     AGTDQGGPHGGHGPRGGAPNGAPNTAPSTTPDSSSGVQ"
     gene            complement(1294..1818)
                     /locus_tag="VF276_15810"
     CDS             complement(1294..1818)
                     /locus_tag="VF276_15810"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX6608367.1"
                     /translation="MNAEAFRQFYAYHFAENRAIWDRYVAPLTPEQFTQGASYSHGSV
                     RDQLVHLMDVDDIWFSELRGVAPAGPLPPATGDERPGLRARWDAIEQDMRVYLAQLRD
                     EMLCDTPITEPDEDRALIVWQVLLHVVNHGTDHRAQLLRRLNDLGVETTSQDYIFYVY
                     AHNAPRSNEGREDC"
     gene            complement(1834..2523)
                     /locus_tag="VF276_15815"
     CDS             complement(1834..2523)
                     /locus_tag="VF276_15815"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX6608368.1"
                     /translation="MGEFIAIERQGEPLRAYLATPEAGKGPGILLCHPWWGVNAFITG
                     LADRLAAEGYTVLAPDLYDGRIATTIPEAEALVGTVEADGGATAVAKEEAALDYLLGR
                     PARTGERVATVGFSMGAAYASWLATLRPEVATVILFYGGAYTDKPGAYHEHTQAALQG
                     HFAPDDEWEPAEPVRELAAAMQAAGGTADIYFYPDTKHWFFEPNRPEYNPAAAQLAWE
                     RALAFLRAHLS"
     gene            complement(2589..>2984)
                     /locus_tag="VF276_15820"
     CDS             complement(2589..>2984)
                     /locus_tag="VF276_15820"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEX6608369.1"
                     /translation="EVTTVDVEGRRATALRSTLDALANVPVAPSVRLLPMFDVYVLAQ
                     SRHIEPMLAPEHKGAVFRPAAWVSAVVLVDGKIAGVWEYATRKDQTRVRVRMFDPPEA
                     PLYREIEAEAGRLAGFLPTRVAVEFADAA"
BASE COUNT          495 a         1059 c          989 g          441 t
ORIGIN      
        1 ttatactgct acaacggcgt gccgactccg cccgtagtcg cttcccaccg ccctgatgtg
       61 acggataaga aaatacctac ccttgaaagc ccgccgcggg aaggggagat cgcgctttaa
      121 gccgtctgga gccccccttc ccgccgcggg aaggggggtt ggggggttag gttggccgcc
      181 caccccgatg ccgcccccgc aaatctcatc cttccctaat gattcgcccg ccgttcccta
      241 atgcccgcgc gctagactga tgatgtgccg cgaaatgacg cggtacccgg caaatgaagc
      301 agaaacaccc aacgaaaagg agcattagga acacctatga acaacaagac gatcctcggt
      361 tccacggcgg ccgtggccct gctcgggctg ggcatgctgg ccggcagcgt ggtgggcacc
      421 agccaggcgt tcgcccagac cggcccggcc acggccagcc ccaccgccgt ggtgaccacg
      481 cccgccgccc cggcgaccac gccatccagc gggccgtcca ccgtcccggt ggcaccgggc
      541 accggacagg cgcccagcgc ggctccggcg cccgccatca cccagcagca agccgagcag
      601 gccgcgctgg ccgcccgccc cggcaatacc gtcgaccaca cgcatctgat gaatgacaac
      661 ggccaggcgt tctgggatgt ggacttctcc aacggcggcg gggtgaagat cgacgcgcag
      721 acgggcgccg tgatcgcggt ggaagctgcc ggcaccgacc agggtggccc gcatggcgac
      781 catggcgccg accaggcggc cctggccgcc caggccaccg tctcgcaggc cgacgcggaa
      841 aaggccgcgc tggccgccag ccccggcaac accattgacc acagccgcct cggcgacgac
      901 aacggcaccg tgtactggga tgtggacttc accaacggcg gcggcgtcga agtgaacgcg
      961 cagaccggcg cgatcatcgc cgtcgaagcc gccggcaccg accagggcgg cccgcacggc
     1021 ggccacggcc cgcgcggcgg cgcgccgaac ggtgccccga acaccgcgcc gagcaccacg
     1081 cccgacagct cgtccggagt gcagtaagcg ccccggcaat cacgaaagga ccaacactat
     1141 cgccacgcgg gtgggccgga tcgatccggc ccacccattg ttgccgtcgt ctctcgttcc
     1201 gcagcccgcg caggcgggct tcgttatggt agcccacccg ttggcgggcc gggcgctccc
     1261 gcccccgcaa ggacgcgaag aatagcactt atatcaacaa tcttcgcgtc cttcgttgct
     1321 gcgtggtgca ttatgggcgt agacgtagaa gatgtaatct tgcgaggtgg tctccacgcc
     1381 cagatcgttg agccggcgca gcagttgcgc ccgatgatcg gtgccgtggt tcaccacgtg
     1441 gagcagcacc tgccacacga tgagggcgcg gtcttcgtcc ggctccgtta tcggcgtgtc
     1501 acacaacatc tcgtcgcgca actgggccag atacacgcgc atatcttgtt cgatggcgtc
     1561 ccagcgggcg cgcaggccgg gccgctcgtc gccggtcgcg ggcggcaacg ggccggcggg
     1621 ggcgaccccg cgcagttcgc tgaaccagat gtcgtccacg tccatgagat gcacaagttg
     1681 gtcgcgcacg gacccatgcg aatagctcgc gccttgcgtg aactgctccg gcgtgagggg
     1741 cgcgacgtaa cgatcccaga tcgcgcggtt ttccgcgaag tgataggcgt agaactggcg
     1801 aaaagcctcg gcgttcatgg cgtcccttcc tcctcaagac agatgtgcgc gcaggaaggc
     1861 gagcgcgcgc tcccaggcca gttgcgccgc cgcgggattg tactcggggc ggttgggttc
     1921 aaagaaccag tgcttggtat cggggtagaa gtagatgtcg gcggtgccgc ccgcggcctg
     1981 catggcggcg gccagttcgc gcaccggctc cgccggctcc cattcgtcgt ccggtgcgaa
     2041 gtggccctgc agcgcggcct gggtgtgctc gtggtacgcg ccgggcttgt cggtgtaggc
     2101 gccgccgtaa aacagaatga cggtcgccac ttcggggcgc agggtggcga gccacgaggc
     2161 gtaggccgcg cccatcgaga agccgacggt tgccacccgt tcgccggtgc gggcgggtcg
     2221 gcccaggagg taatcgagcg cggcttcctc tttggcgacc gccgtggcgc ccccgtcggc
     2281 ttcgaccgtc ccgacgaggg cttcggcctc ggggatggtg gtggcgatgc gcccgtcgta
     2341 gaggtcgggg gccagcacgg tgtagccttc ggcagccagc cggtccgcga gaccggtaat
     2401 gaacgcgttc acgccccacc aggggtgaca cagcaggatg cccggccctt taccggcctc
     2461 cggtgtcgcc aggtaggcgc gcagcggctc gccctgtcgc tcgatcgcga tgaattcgcc
     2521 catgtgattg gtgctccttt ctcctgtgca atagcgaggt agcgcggcac aatcagcccc
     2581 accccgcgct acgccgcatc cgcaaattcg accgccacgc gggtgggcag aaagccggcc
     2641 aggcgaccgg cctcggcctc gatctcccgg tacagcggcg cttccggcgg gtcaaacata
     2701 cggacccgca cccgcgtctg gtccttgcgc gtggcatact cccacacgcc ggcgatcttg
     2761 ccgtcgacca gcaccacggc gctcacccac gcggcgggcc ggaacacagc gcctttgtgc
     2821 tcgggcgcca gcatcggctc gatatggcgg ctctgcgcca gcacgtacac gtcgaacatg
     2881 ggcagcaggc gcacgctcgg cgccaccggc acgttcgcca gcgcgtccag cgtggagcgc
     2941 agcgcggtgg cccgccggcc ctccacgtcc accgtcgtca cctc
//
LOCUS       DASUYC010000966         5475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1815113, whole genome shotgun sequence.
ACCESSION   DASUYC010000966 DASUYC010000000
VERSION     DASUYC010000966.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5475
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1815113"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1046)
                     /locus_tag="VF276_15825"
     CDS             complement(<1..1046)
                     /locus_tag="VF276_15825"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6608370.1"
                     /translation="MAAELPANAPRAVALAQHVDTTRLGHGQMFAISAMWFALSVVWG
                     GLLTLVVPTQVEDILRRQGLSSVDVTAQKGLFVGLVIAAGAVIALVVPPLVGAYSDQS
                     THRMGRRRPFVLVGVLITLAGLLGMITPPSMLIYGVFYLLLQFGSNTAVAAYQGLIPD
                     LVPEQQRGHASGWLGMMTITGNIVGLLLGSVGLARAHAEDLLTAEQQWLVYGVIIAIL
                     VVFLGITLFGVRETPLVATAPRAGLGAILKSLWIDPRRYPDFAWVWITRFLVTMGFNT
                     VQFFLLYFLQDVIGIGRDKLSTYGGFMFLGLLLSAAVTSIVSGRISDRKGRKPLIYVA
                     GGVMALMGVAFIFL"
     gene            complement(1241..2617)
                     /locus_tag="VF276_15830"
     CDS             complement(1241..2617)
                     /locus_tag="VF276_15830"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF005914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEX6608371.1"
                     /translation="MTTPTNDELTGDEQQAVLAELDEFLRIPSISTLSEHRPDMERAA
                     TWVAEFLRRAGLDHARVIPTNGHPLVYADWLHAPGQPTILIYGHYDVQPPDPLDAWQS
                     PPFEPTVRDGNLYARGAADDKGQIFIHMKTVERLLRDGGTLPVNVRFLIEGEEETSGK
                     SIEEYVQAHADELACDAVLISDSPMFAPDQPTIGVGLRGALFTEVTVTTGRHDVHSGL
                     YGGAAPNAIHALCHILDALIDADGRITIPGYYDAVRMPSDAERAQWAALPFDETAYRE
                     QEIGAPALVGEAGFPVLERTWARPTLDVNGIWGGFTGEGSKAIIPAQASAKVSMRLVP
                     DQEPRQILDAFTQYVEQLCPAGARVTVRALTIGAPVLVDPDTPIMQAAGAALRDVFGK
                     PAVYTRMGGSIPIVSLFMERLHAPTVMLGFGLPDDNLHAPNEKMRLANIYDGIRASTA
                     FLRRLHRA"
     gene            complement(2651..4867)
                     /locus_tag="VF276_15835"
     CDS             complement(2651..4867)
                     /locus_tag="VF276_15835"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HEX6608372.1"
                     /translation="MSDPWASGMNKLVLREGDMFMVSDQRGDMAVGQTLGLYYHDTRY
                     LSVYQLALNGQTPTLLSSSCEQDFMANLQFTNPTLPQEGKPDVLPHTISLRRNRLVRD
                     GLRERIGLMNYQRTPLTVQITLEMGADFRDMFDVRGYPRAARGEIHPPTWSDSLLTFS
                     YTGLDEKERYTRVSFDPPPSRVEIQPVEGPVLGEPGTVIPYGVERTQTQTVLPPFVRA
                     TWDVTLAPGRPWAVTLLVAPEGPPEPAPSPQFDEDARRLRQQYVQWETGWGVETDHAV
                     INRLLQRSLADLRVLSLPVPGGFVPVAGIPWFAVPFGRDSLITALQTLVFKPDIAVGT
                     LRFLAAYQGTQVDKRREEEPGKILHEIRYGEMARLGEIPHTPYYGTVDATPLFLMVFA
                     QAMRWLDDDALYEELLPNVRAAFDWIDQYGDVDGDGYIEHHRGSHSQGSLSNQGWKDS
                     KDSLQWPDGTYPVGPVALAEVQGYVYAARKWCSDVLARRGEKEWAAQLARQAAELKTR
                     FNQDFWMEDQGFFAQALEGDKRPIPTVASNPGHCLYTDVIDDAHREKVVERLLSPDME
                     CGWGIRTVSSTHPSFNPMSYHNGSVWPHDNAIIAAGMMRRGYVDGALRVLNAIVDAAL
                     HFRYLRLPELYCGFARDLRYYNIPAEYPVSCSPQAWAAGTMLHLFQTMLGLDADANLG
                     ILRLAPHFPDTLHQAQIRNLRVGRHTVDLLVARSGPGAPPTVQVLRNPGGIDVVLR"
     gene            complement(4894..>5475)
                     /locus_tag="VF276_15840"
     CDS             complement(4894..>5475)
                     /locus_tag="VF276_15840"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6608373.1"
                     /translation="AIFCWSAAISAMQDFFTLYGRDVLGIDAGTAAQMLTLFAAAAVL
                     FAIPGGLLATRFGRRRTVGACLVILAVLFTAAYATTSLLFDQIMLVIAGAAYTTIIVS
                     ALPMVLDAAPTNDTGTYTGVYYVFGATAGIVGPPLAGWLIDVTGTYRSVFLFGPIFMT
                     LALILILRNRGGEAQPAGAPVEMADPAPSATGG"
BASE COUNT          917 a         1812 c         1870 g          876 t
ORIGIN      
        1 ctgaggaaga taaacgccac gcccatcagc gccatgacgc cgccggccac gtaaatcaac
       61 ggcttgcgtc ctttgcgatc ggagatgcgt ccgctgacaa tcgacgtgac cgccgcgctg
      121 agcagcagcc ccaggaacat aaacccgccg taggtcgaga gcttgtcgcg cccgatgccg
      181 atcacatctt gcaggaagta gagcaggaag aactgcacgg tgttgaagcc catcgtcacc
      241 aggaagcgcg tgatccagac ccaggcgaag tcgggatagc ggcgcggatc gatccacagg
      301 ctcttgagga tggcgcccag cccggcgcgc ggggccgttg cgacgagcgg cgtctcgcgc
      361 acgccgaaca gcgtgatgcc caggaacacg accaggatgg cgatgatcac gccgtagacc
      421 agccattgct gctcggcggt gagcaggtct tcggcgtggg cgcgcgccag gcccacgctg
      481 cccagcagca ggcccacaat attacctgtg atagtcatca tgcccaacca gccgctggcg
      541 tggccgcgct gctgctcagg caccaggtcg gggatgaggc cctggtacgc cgcgaccgcc
      601 gtgttcgagc cgaattgcag cagcaggtag aaaacgccgt aaatgagcat gctgggcgga
      661 gtgatcatgc ccagcagccc ggccagggtg atcagcacgc ccaccagcac aaacgggcgc
      721 cgccggccca tgcggtgggt ggactggtcg ctgtaggcgc ccaccagcgg cggcaccacc
      781 agcgcgatca ccgcgcccgc cgcgatcacc aacccgacga agaggccttt ctgcgccgtc
      841 acatcgacgc tgctcagacc ctggcggcgc aggatgtcct ccacctgagt cggcacgacg
      901 agcgtgagca ggccacccca gaccacgctt agtgcgaacc acatcgcgct gatggcgaac
      961 atctggccgt ggcccaggcg cgtggtgtcc acgtgctggg cgagagcgac ggcgcggggg
     1021 gcgttggcgg ggagttcggc ggccatgggc agtctcctta gcgcttggga agccggggac
     1081 cgagggtatg ccccgattgt agcacaggat tcttgcccgc gcaatggccc tgtcttacgc
     1141 ccgttcgcaa tttcaccgcg aaggcacgaa ggacgagaaa gttagtaatg taacttctat
     1201 tcttcgcgtt cttcgcgcct tcgcggtgcc ccgtagagcg ttacgcgcgg tgcagccgcc
     1261 gcaggaacgc cgtcgaggcg cggatgccgt cgtagatgtt ggcgaggcgc atcttctcgt
     1321 tcggcgcgtg gaggttgtcg tcgggcaggc caaagccgag catgacggta ggcgcgtgca
     1381 gccgctccat gaacagcgag acaatcggga tcgagccgcc catgcgcgtg tagacggcgg
     1441 gcttgccgaa cacgtcgcgc agggccgcgc ccgccgcctg catgatcggc gtatcggggt
     1501 ccaccagcac cggcgcgccg atggtcagcg cccgcaccgt gacgcgggcg cccgccgggc
     1561 agagctgctc cacatactgg gtgaacgcat cgagaatctg ccggggctcc tggtcgggca
     1621 cgaggcgcat gctcaccttg gcgctcgcct gcgccggaat gatagccttg ctgccctcgc
     1681 cggtgaagcc gccccagatg ccgttcacgt cgagcgtggg ccgcgcccag gtgcgctcca
     1741 gcaccgggaa gcccgcttcg cccaccagcg ccggcgcccc gatctcctgc tcccggtacg
     1801 ccgtctcgtc gaagggcagc gcggcccact gggcgcgctc ggcgtccgag ggcatgcgga
     1861 ccgcgtcgta gtagccgggg atggtgatcc gcccgtcggc gtcgatgagc gcgtcgagga
     1921 tgtggcacag ggcgtggatg gcgttgggcg ccgcgccccc gtacagcccc gagtggacat
     1981 cgtggcgccc ggtggtcacg gttacttcgg tgaacagcgc gccgcgcagc cctacgccga
     2041 tcgtcggctg gtccggcgcg aacatcgggc tgtcgctgat cagcacggcg tcacaggcta
     2101 gctcatcggc gtgcgcctgc acatactcct cgatgctctt gccgctggtc tcctcctcgc
     2161 cttcgatcaa gaagcgcacg ttcaccggca acgtgccgcc gtcgcgcagc aggcgctcga
     2221 ccgtcttcat atggatgaag atctggccct tatcgtcggc ggcgccccgc gcgtagagat
     2281 tgccgtcgcg cacggtgggc tcgaaaggcg ggctctgcca ggcgtccagc gggtcgggcg
     2341 gttggacatc gtagtgccca tagatcaaaa tcgtcggctg gccgggcgcg tgcagccagt
     2401 cggcgtaaac cagcgggtgg ccgttggtcg ggatcacgcg ggcgtgatcg aggcccgcgc
     2461 ggcgcaggaa ctcggccacc caggtggcgg cgcgttccat atcgggccgg tgctccgaca
     2521 gcgtgctgat cgaggggatg cgcaggaact cgtccagttc cgcgagcacc gcctgctgct
     2581 cgtcgccggt taactcgtca ttggtagggg tggtcataag caaggtcctc gtttcttagg
     2641 cgctagcgga tcagcgcagc accacgtcga tgccgcccgg attgcgcagc acctggacgg
     2701 tgggtggcgc gcccggcccg ctgcgcgcca cgagcaaatc cacggtatgc cgccccacgc
     2761 gcaggttacg gatctgcgcc tggtgcaagg tgtcagggaa gtgcggcgcc aggcgcaaga
     2821 tgcccaggtt ggcgtcggcg tcgaggccga gcatggtctg gaacaggtgg agcatcgtgc
     2881 ccgccgccca ggcttgcggg ctgcacgaca ccggatattc cgccgggata ttgtagtagc
     2941 gcaggtcgcg ggcaaagccg caataaagct cgggcaggcg caggtagcgg aagtgcagcg
     3001 ccgcatcgac aatagcgttg agcacgcgca acgccccatc gacatacccg cggcgcatca
     3061 tgcccgcggc gatgatcgcg ttgtcatgcg gccagaccga gccgttgtgg tagctcatcg
     3121 ggttgaagct gggatgtgtg ctgctcaccg tgcggatgcc ccagccgcac tccatgtccg
     3181 ggctgagcag ccgctccacg accttctcgc ggtgcgcgtc gtcgatcacg tcggtataga
     3241 ggcagtggcc gggattggag gcgacggtcg ggatgggccg cttgtcgcct tcgagcgcct
     3301 gcgcgaagaa gccctgatct tccatccaga agtcctggtt gaagcgggtt ttgagttcgg
     3361 cggcctgccg ggccagttgc gcggcccact ccttttcgcc gcgccgagcc agcacgtcgc
     3421 tgcaccactt gcgcgccgcg tagacatatc cctgaacctc ggccaacgcc accgggccca
     3481 ccgggtaggt gccgtcgggc cattgcagcg agtccttgga gtccttccag ccctggttgc
     3541 tcaggctgcc ctggctgtgg ctgccgcggt ggtgctcgat gtagccgtcg ccgtccacgt
     3601 cgccgtactg gtcgatccag tcgaacgccg cgcgcacgtt gggcaacaac tcctcgtaca
     3661 gcgcgtcgtc gtccaaccag cgcatggcct gggcaaagac catcaggaac agaggcgtgg
     3721 cgtcaaccgt gccgtagtag ggcgtgtgcg ggatctcgcc cagccgggcc atctcaccgt
     3781 agcggatctc gtgcaggatc ttgcccggct cttcctcgcg gcgcttatcg acctgcgtgc
     3841 cctggtaggc ggccaggaag cgcaacgtgc ccacggcgat atcgggcttg aagacgagcg
     3901 tttgcagggc cgtgatcagc gagtcgcggc caaagggcac cgcgaaccag gggatgcccg
     3961 ccaccggcac aaagccgccg ggcaccggca gactcagcac acgcaggtcg gccaggctgc
     4021 gttgcagcag gcggttgatg acggcgtggt cggtctccac gccccagccg gtctcccact
     4081 gcacatactg ctgccgcaac cgccgcgcgt cctcgtcaaa ctgcggcgac ggcgcgggct
     4141 cgggcgggcc ttcgggcgca accagcagcg tcaccgccca gggccggcca ggtgccagcg
     4201 tgacatccca ggtggcgcgc acgaacggcg gcaacaccgt ctgtgtctgc gtgcgctcga
     4261 cgccgtaggg gatcaccgtg cccggctcgc ccagcaccgg cccctccacc ggctggatct
     4321 ccacccggct cggcggcggg tcgaagctca cgcgggtata gcgttctttc tcgtccaggc
     4381 cggtgtagct gaaggtaagc aggctgtcgc tccaggtggg cgggtggatc tcgccgcggg
     4441 cggcgcgggg atagccgcgc acgtcgaaca tgtcgcggaa gtcggcgccc atctccaggg
     4501 tgatttgcac cgtcagcggg gtgcgctggt agttcatcag gccgatgcgc tcgcgcaggc
     4561 cgtcgcgcac cagccggttg cgccgcaggc tgatcgtatg cggcagcaca tcgggcttgc
     4621 cctcctgcgg cagcgtcggg ttggtgaact gcagattcgc catgaagtcc tgctcgcagg
     4681 atgacgagag cagcgtcggc gtttgcccgt tcaaggcgag ttgatacacg ctgagatagc
     4741 gggtgtcatg ataatagaga ccgagagtct ggccgacggc catatcgccg cgctggtccg
     4801 agaccatgaa catgtcgcct tcgcgcagca cgagtttatt catcccgctg gcccagggat
     4861 cgctcattgc atcctcctgt caggttgcgt gggtcaaccg cccgttgctg agggtgccgg
     4921 gtcagccatt tcgaccggcg cgcccgccgg ttgggcctct ccgccacgat tgcgcaggat
     4981 caagatcagg gccagcgtca tgaaaatcgg gccaaatagg aaaacagaac ggtaagtgcc
     5041 ggtcacatcg atcagccacc cggccagcgg cggcccgacg atgcccgcgg tggccccgaa
     5101 cacgtagtag acgccggtat acgtgcccgt gtcgttcgtc ggcgcggcat ccagcaccat
     5161 cggcagcgcg ctcacgatga tcgtggtgta cgccgccccg gcgatcacca gcatgatctg
     5221 gtcgaacagc aggctcgtcg tggcgtaggc cgcggtgaac agcaccgcca ggatgaccag
     5281 gcaggcgccg accgtgcgcc gccggccaaa ccgtgtggcg agcagtccgc cggggatggc
     5341 gaagagcacc gccgccgcgg cgaacaaggt cagcatctgc gcggcggtgc cggcgtcgat
     5401 gccgagcacg tcgcgcccgt agagcgtgaa aaagtcttgc atcgcgctga tcgccgcgct
     5461 ccagcagaag atcgc
//
LOCUS       DASUYC010000967         2534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_392634,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000967 DASUYC010000000
VERSION     DASUYC010000967.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392634"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1613)
                     /locus_tag="VF276_15845"
     CDS             complement(<1..1613)
                     /locus_tag="VF276_15845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608374.1"
                     /translation="MSRLRPRLTLPVRPLVCLLLIAASLASLGLLPPGDSAAAPRDAI
                     DAAAQAGLVRLSFYFQQNLNSGCTPRPGRANCAGSLSPDQNGGVLSAIRAALPTGFNT
                     FDAFYVDGQGTRYGTNEVGGVYSVPFEDPGQRGVAGVQVRSTEGGTPGPFVTYTFAGL
                     RTILHPPSDIWLPPSPTPLPPGPSGALRLAAPTSADQLTIWPLVLFTDTIQVGSVTAL
                     LPFTTTYTVNAWSRLCYSGYTGGGINCSGIDIEGWFNYGFDPSQGVALWLENMNDPMR
                     GQGQVATYTSLPAGWDGTNSDSTGAWMASPTGNVSYQSCAAMQRAGDQHQRVLLPIAD
                     RSNNAAGSNTAYHIRNFAAFEVLHPTSGESCSPNNIRLQGRFLGYTWRNTEATATGDL
                     GAAYSNGQIVLPFAPSDPPAPPTATATATATPTAAPATGTPTATATPPATNTAIPTAT
                     ATATATATETATATETTTAIPTVTVTETPTVTATATATAVLPTDTATPTPVPPSATAT
                     AVPATATAPPPTDTSTPSPPIVTVTAPPS"
     gene            1831..>2534
                     /locus_tag="VF276_15850"
     CDS             1831..>2534
                     /locus_tag="VF276_15850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608375.1"
                     /translation="MNRSPARLGTLLGVVLLPLLALAGVLWHWGAGGTEWRLSNVSGV
                     SGEWPAGGSLGQSFTPRYPGLSAVGLQIATYQRVNTGTLDLLLLGPGDRELAHATLAK
                     PALVDNGVAWFGFAPLPAATVGQTLRLELSDPTAVPGRNTVTVYINDRDPYGGGTAYL
                     NGAPVPAVDLAFGMRYAVTPPELWAAQIAAWARAVAPGLVALAALAALGWLAGCAWSS
                     ATRRLCPAATPSPSIST"
BASE COUNT          370 a          788 c          974 g          402 t
ORIGIN      
        1 tggcttggcg gcgcggtcac tgtgacgatg ggcggtgaag gcgtcgaggt atcggtgggc
       61 ggcggtgcgg tcgccgtagc cgggaccgct gtggccgttg cgctgggagg caccggggtc
      121 ggcgtagccg tatctgttgg caatactgcc gtcgcggtcg ccgtcgctgt tactgtcggt
      181 gtctcggtca ctgtcacggt cgggattgcg gtggtggtct ccgtcgcggt ggccgtttcc
      241 gtcgccgtgg cggtggccgt ggccgtcgct gtggggatgg ccgtgttggt ggcgggcggg
      301 gtggccgtcg ccgtgggcgt gccggtggcg ggcgcggcgg tgggcgtggc cgtcgcggtg
      361 gcggtagccg tgggcggggc cggcgggtcg gacggagcga acggcagcac gatctggccg
      421 ttgctgtagg ccgcgcccag gtcgccggtg gccgtcgcct cggtgttgcg ccaggtgtag
      481 cccaggaagc ggccctggag ccggatattg ttaggagagc aggattcgcc cgacgtggga
      541 tgcagcacct cgaatgcggc gaagttgcgg atgtgatagg ccgtgttgct gcccgccgcg
      601 ttgttactgc gatccgcgat aggcagcaac acccgctggt gttgatcgcc ggcccgctgc
      661 atcgccgcac aggattgata gctgacattg cccgttggtg aagccatcca ggcaccggtt
      721 gagtccgagt tggtgccatc ccagccggcg ggtagcgaag tataggtagc gacttgcccc
      781 tgcccgcgca tggggtcgtt catattctcc aaccacaggg caaccccctg gcttggatcg
      841 aatccatagt taaaccagcc ttcgatgtca atgccggagc agttgatgcc gcccccggta
      901 tagccggagt agcagagccg cgaccacgca ttcaccgtgt acgtggtggt gaacggcagc
      961 agcgccgtca cgctgcccac ctggatcgta tcggtgaaga gcaccagcgg ccagatggtg
     1021 agctgatcgg cgctcgtggg cgcggcgagg cgcagcgcgc cgctaggacc gggcggcaat
     1081 ggcgtgggcg agggcggcag ccagatgtcg ctcggcggat gcaggatcgt gcgcaggccg
     1141 gcaaaggtgt aggtaacgaa ggggccgggg gtgccgcctt cggtgctgcg gacttgcaca
     1201 cccgccacgc cacgctgccc aggatcctcg aagggcacgc tgtacacgcc gcctacctcg
     1261 ttggtgccat agcgcgtgcc ctggccatcc acgtagaagg cgtcgaaggt gttgaagccg
     1321 gtgggcagcg cggcacggat agccgacagc acgccgccat tctggtcggg ggatagggag
     1381 ccggcacagt tggcccggcc ggggcggggc gtacagccgc tgttcaggtt ctgctgaaag
     1441 tagaagctga ggcggaccag accggcctgc gcggcggcgt cgatggcatc gcgcggggcg
     1501 gcggcggagt cgccgggggg cagcagaccc aggctagcga gagaagcggc gatcagcaac
     1561 aggcaaacga gcgggcgaac aggcagcgtg aggcgaggac gaagccgcga catgaggacc
     1621 atcctttgca acgagtggta tagtgacagc cggcgggagc cgggcggagc gccctggatg
     1681 ggagtaccgg cctattatac gcgatcacgt gtgccgttgc catgcaccac ccggccccta
     1741 gaggcaggcc ggtagtcctt cgtagtacgc gcgggcgggc cgggtgcccc tttgccgtgg
     1801 gagcaagaga ccggctataa tggcgcccat atgaaccgtt cacctgcccg cctgggaacc
     1861 ctgctcggag tcgtgctgct gccgctgctg gcgctggcgg gcgtgctgtg gcattggggc
     1921 gccgggggca ccgagtggcg gctgagcaac gtcagcgggg tcagcggcga gtggccggcg
     1981 gggggcagcc tgggccagag cttcacgccg cgctatcccg gcctgagcgc ggtggggctg
     2041 caaatcgcca cctaccagcg ggtgaacacg ggcacgctcg acttgctcct gctcggcccc
     2101 ggcgaccgcg aactggcgca tgccacgcta gcaaaacccg ccctggtgga caacggcgtg
     2161 gcctggttcg gcttcgcgcc gctgcccgcc gcgacggtgg gccagacgct gcgcctggag
     2221 ctcagcgacc cgacggctgt gcccggccgc aacaccgtca ccgtctatat caacgaccgc
     2281 gacccctacg gcggcggcac ggcctatctg aacggcgcgc cggtgcccgc cgtggacctg
     2341 gccttcggca tgcgctacgc cgtgacgccg cccgaactct gggcggcgca gatcgcggcg
     2401 tgggcgcgcg ccgtggcgcc ggggttggtg gcgctggccg cgctggccgc gctgggctgg
     2461 ctggcgggct gcgcctggag ctcagcgacc cgacggctgt gcccggccgc aacaccgtca
     2521 ccgtctatat caac
//
LOCUS       DASUYC010000968         2750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1340628, whole genome shotgun sequence.
ACCESSION   DASUYC010000968 DASUYC010000000
VERSION     DASUYC010000968.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1340628"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1731
                     /locus_tag="VF276_15855"
     CDS             <1..1731
                     /locus_tag="VF276_15855"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX6608376.1"
                     /translation="GLPQVWTVAVPGAWPVQVTFGAERVLYAHAAHHDGRMILGSDRG
                     GNEHVQLTLLSADGATQTPLDPNPAVIHTFGGWAPDDRAIAYAANTRHPAFFDIYTLA
                     LDAPDAAPRLVYQQDGSNSVAAWAPDGQSLIIQHTEKPANQDLYLVPLDGSPPRHLTP
                     HSGDAIYEHVEWAPDGRNLYLITDEEREFAALVRLDLATGDRETLVAPDWDLDFCFLS
                     PDGARLAYSVNVDGYSELHVRDLAAGTDRQPAGLPRGNVGYTGSSASAYAPELAWAPD
                     SRRLAFNVTAPTRNLEIWQWNLDSDTVEQVTRNGMAGIPAETFVDPELVRYPTFDGRE
                     VPAFFYRPADAADGPLPCVVQVHGGPEGQARVAFDPVIQYFVHRGYAVLAPNVRGSMG
                     YGREYTHLDDVRKRMDSVHDLAAAVEWLRGSGQVAPDRIAVMGGSYGGFMTLAAVTTY
                     PDLWAAGVDIVGIANFVTFLENTGPWRRKLREAEYGSLEADADFLRTISPIHHVDQIT
                     APLMVIHGANDPRVPVGEAEQIVEILRARQHPVEYLRFSDEGHGVVRLHNRLVAYPAI
                     ADFLDQYLGG"
     gene            1860..>2750
                     /locus_tag="VF276_15860"
     CDS             1860..>2750
                     /locus_tag="VF276_15860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608377.1"
                     /translation="MPARSWRAVLLAALVLVPAVLVLGRLGLFATHSADMIAFPWQID
                     FDEGIILHSSWLLAQGRNPYPPLGPDHFISSLYPPLFYILNAVALKLWGLNLWSGRLL
                     TLLGTLLAAGALVGWVWAETRARLAGITAAALWLALGPVIVWATFYKQDILALGLGVL
                     GGAMVAAACPPDRAETTGGVRWGGLPRLAWAAILPLVLACWTKQSTLAPLAAAGLFLL
                     ARDWRSGLRWNMGAAATLLVPFLIANVASHGGLATHLFLPSAEELSGARLGKNLGALW
                     TEHWPLVVVAAVVALGLLLTA"
BASE COUNT          380 a          993 c          952 g          425 t
ORIGIN      
        1 ggcctgccgc aggtctggac ggtggccgtg ccgggggcct ggcccgtgca ggtcactttc
       61 ggcgccgagc gggtgctgta tgcgcacgct gcccaccacg acggccggat gatcctgggc
      121 agcgaccgcg gcgggaacga gcatgtgcaa ctgaccttgc tcagcgcgga cggcgccacg
      181 cagacgccgc tcgaccccaa cccggcggtg atccacacct tcggcggttg ggcgcccgac
      241 gaccgcgcca tcgcctatgc cgccaacacg cgccaccccg cctttttcga catctacacc
      301 ctggcgctcg atgcgccgga tgccgcgccg cggctggtct accagcaaga cggctcgaac
      361 agcgtggctg cctgggcacc cgacgggcag tcgctcatca tccagcacac cgagaagccg
      421 gcgaaccagg acctctacct ggtgccgctc gacggcagcc cgccgcggca cctgaccccg
      481 cacagcggcg acgcaatcta tgagcacgta gagtgggcac cggacgggcg caacctctac
      541 ctgatcaccg acgaagagcg cgagttcgcg gccctggtgc ggctcgatct ggcgaccggc
      601 gaccgcgaaa cgctggtcgc gcccgactgg gatctcgact tctgcttcct gtcgcccgac
      661 ggcgcgcgcc tggcctacag cgtcaacgtg gacggctact cggaactgca cgtgcgcgac
      721 ctggccgccg gcacggatcg ccaaccggcg gggctgccgc gcggcaacgt gggctacacc
      781 ggcagtagcg cctcggccta cgcgcccgaa ctggcctggg cacccgacag ccgccgcctc
      841 gccttcaacg tcaccgcgcc cacgcgcaac ctcgaaatct ggcagtggaa cctggacagc
      901 gacacggtgg agcaggtgac gcgcaacggc atggccggca tcccggcgga gaccttcgtc
      961 gatcccgaac tggtccgcta cccgacgttc gacgggcgcg aggtgccggc tttcttctac
     1021 cggcctgctg acgcggcgga tgggccgctg ccctgcgtcg tgcaggtcca cggaggcccg
     1081 gaggggcagg cgcgcgtcgc cttcgacccg gtgatccagt acttcgtcca ccgcggctat
     1141 gcggtgctgg cgcccaacgt gcgcggcagc atgggctatg gccgcgagta cacgcacctg
     1201 gacgatgtcc gcaagcgcat ggactcggtg cacgacctgg ctgccgccgt ggagtggctg
     1261 cgcgggtcgg ggcaggtggc gcccgaccgc atcgccgtga tgggcggcag ctacggcggc
     1321 ttcatgaccc tggccgctgt gacgacttat cccgacctct gggcggcggg cgtggacatc
     1381 gtcggcatcg ccaacttcgt gaccttcctg gagaacaccg gcccctggcg gcgcaagctg
     1441 cgggaggccg aatacggcag cctggaggcc gacgccgact tcctgcgcac catcagcccg
     1501 atccaccacg tggaccagat caccgcgccg ctcatggtca tccacggggc caacgacccg
     1561 cgcgtgcccg tcggcgaagc cgagcagatc gtggagatcc tacgcgcccg gcagcacccg
     1621 gtggaatacc tgcgcttctc cgacgagggc cacggcgtgg tccgcctgca taatcgcctg
     1681 gtggcctacc cggccatcgc cgatttcctg gaccagtacc tgggcggcta gaggcacggc
     1741 atgggcatcc ggatccacta ttgcggcagc gccgcgccta tccgccgctc aagggcgggc
     1801 tggtgatggc aacccgcgcc accttgccgc ggcccgtggg cgacaggtat aatcgcccta
     1861 tgcctgcgcg ctcgtggcgg gccgtcctcc tggcggcatt ggtcctggtg ccggcggtcc
     1921 tggtgttggg ccggctgggg ctgttcgcta cccacagcgc cgacatgatc gccttcccct
     1981 ggcagatcga cttcgacgag ggtatcatcc tgcactcgtc gtggctgctg gcgcagggtc
     2041 gcaaccccta tccgccactc ggccccgacc acttcatctc gtcgctctac ccgccgctgt
     2101 tctacatcct caacgccgtg gcgctcaagc tgtgggggct gaacctctgg agcgggcgcc
     2161 tgctcaccct gctgggcacg ctcctggcgg cgggcgcgct ggttggctgg gtgtgggccg
     2221 agacccgcgc gcggctggcc gggatcacgg cggcggcgct ctggctggcg ctcggcccgg
     2281 taatcgtctg ggccactttc tacaagcaag acatccttgc gctgggcctg ggcgtgctgg
     2341 gcggggccat ggtcgcggcg gcttgcccac ccgaccgggc cgagaccacc ggcggggtgc
     2401 gctggggcgg cttgccgcgg ttggcctggg cggcgatctt gccgttggtg ctggcctgct
     2461 ggaccaagca atcgacgctg gccccgctgg cggcggcggg actctttctg ctggcgcgcg
     2521 actggcgcag cggactgcgc tggaacatgg gcgcggcggc gacgctgctt gtgcccttcc
     2581 tgatcgccaa cgtcgcctcg cacggcggcc tcgccacgca cctgttcctg cccagcgcgg
     2641 aggaactgtc gggcgcgcgg ctgggcaaga acctgggcgc gctctggacc gaacactggc
     2701 cgctggtcgt cgtggcggcg gtcgtggcgc tgggcctgct gctcacggcg
//
LOCUS       DASUYC010000969         4503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_681139,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000969 DASUYC010000000
VERSION     DASUYC010000969.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4503
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681139"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="VF276_15865"
     CDS             complement(<1..900)
                     /locus_tag="VF276_15865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608378.1"
                     /translation="MRRLIPAQWPAMPRLLQTPALRALWRYRVALAGLSALALALWGE
                     QMMREIPGNLAVGTALVQIAVLLVGLVAWVGQARRLAPPALADAPPAEPATTSLPVRR
                     AGQAGAVQVAGVTPEPAAPAAPGGLRATWASFRDVRARLGWAGTAVGLLIVAGLAGWC
                     LLLLRADFGDPLAPWIWLAALVALTLTFAGMRPAAPGLVPHDPAEPATEPPMTRGEWL
                     VLGLILLVAVVVRVWNLETIPMGPYSDEGDRAVDARHINRGETVNAAPFAFFGTGWWG
                     VPSLYFWLVAQSLKVFGDTLAGAR"
     gene            complement(900..1370)
                     /locus_tag="VF276_15870"
     CDS             complement(900..1370)
                     /locus_tag="VF276_15870"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX6608379.1"
                     /translation="MASGPERLQAVLDELDLGVQVIRVPGSAKTAILAAESVGCPVGA
                     IVKSLVFVAGDDPVLVLCPGDRRVDTAKLADLRRVDPAAISMAPAALVREVTGYAIGG
                     VPPLGHVSALPVYMEQALLDWPTVYAAAGAPDALFPIDPNVLQQITGAVVVQVT"
     gene            1512..3215
                     /locus_tag="VF276_15875"
     CDS             1512..3215
                     /locus_tag="VF276_15875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX6608380.1"
                     /translation="MHTAANSEPPRAAPARPSFPIPWVDLRNLYIILAGCVALLVLLP
                     PEHEYPIIDDWIYAGSVQHQVDTGAFAMPPESQANLVGLTLWGTLWARLFGAGYTTLT
                     YSTLALALLGLLAFYGLARRVGTPPNAALLGVGLLAVNPLFLHLSASFMTDVPFLALV
                     LVACYGYVRGLQCGQWGWLLVAGICAGWGFLIRQFAILVPAGFVGYLLLEGLPSRRWR
                     RLEMLAVIGPPALIVLGWAIWSRDTATTPFTIQADQHRAAFVLQPAWVEVFLLRTLTI
                     LPLTALSAWAAIILPRRRWWLVGLWAVLVAGAEWIAANLSHVWIANTQPPFTFRLGPL
                     ALDFPQQTYPFGSEGSILREGGIDFFQYRQEPMWSPEAWHLLFALGLILGVLLLAKMT
                     NSLIDWLRAWWRERDLSPLSGVYLTGLATFGVTLALGGDFYDRYLLGFVAFVILFVVR
                     GAAGWSRRAWAYTLVAFALWAGFSVLLKADSIDHDNARWQAGQWLAARAGPVQMGFDW
                     NNVHPAGNGTYEIADMPIAGFRTEARFPYTSRLSGFTTRYVLAQARADMPALPPPRPG
                     R"
     gene            3283..>4503
                     /locus_tag="VF276_15880"
     CDS             3283..>4503
                     /locus_tag="VF276_15880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6608381.1"
                     /translation="MVTSSYPKYPGDVTAPFIQSIATYVAAQGHEVHVLAPWHPDLRH
                     QPVENGVHLHFYKYAPLPNLNIWGYAESLEADVRVRNAIYPLTPLVTLISFFALMRLT
                     GRIKFDVLQAHWVIPNAPVAAVVARLRGLPLVISLHGSDVFVASQNRVLAWAARQCFR
                     AADAVTACSEDLRERALALGAAPRRTRLVPYGVDPCAFAPIEDAGADLRERLGLAPGT
                     PVVLAIGRLVYKKGFEYLIQAAPTILARHPQAQIYIAGQGDLQRELQAQAARVGVAGH
                     VHLPGKVSHDDVPTYLSGCDVFVLPSVVDQSGNVDGLPNALLEAMAAGCAVVASNVAG
                     VPLAVQSGENGLLVPQKDPGRLAEAVSTLLGDAALRAEMGAAARRKIEQELNWPHIAR
                     MFESIYRVASARRTR"
BASE COUNT          631 a         1624 c         1546 g          702 t
ORIGIN      
        1 acgcgccccg gccagcgtat cgccgaagac cttgagcgat tgggcgacca gccagaaata
       61 gaggctgggc acgccccacc agccggtgcc gaaaaaggcg aacggcgccg cgttcactgt
      121 ttcgccccgg ttgatgtggc gggcgtccac cgcccggtcg ccttcgtcgc tgtatggccc
      181 catggggatg gtttcgaggt tccagacgcg gacgaccacc gcgacgagca ggatcaggcc
      241 caggaccagc cattcgccgc gcgtcatggg cggctcggtc gccggctcgg cggggtcgtg
      301 gggcaccagg ccgggggcag cggggcgcat cccggcgaag gtgagggtca gggcaaccag
      361 ggccgccagc cagatccagg gcgccagcgg gtcgccgaaa tcggcgcgca gcagcagcaa
      421 gcaccagccc gccagcccgg cgacgatcag cagaccgacc gcggtgcccg cccagcccag
      481 gcgggcgcgc acatcgcgga agctggccca ggtcgcgcgc agcccacccg gcgcggcggg
      541 agcggcgggc tcaggcgtca ccccggccac ctgcacggcg cccgcctgac cggcccgccg
      601 caccggcagg ctcgtggtgg ccggttccgc gggcggcgca tcggcgagcg cgggcggggc
      661 caggcgccgg gcctgcccca cccaggccac caggccgacc agcagcacgg cgatctgcac
      721 cagcgcggta cccacagcga gattgccggg gatctcgcgc atcatctgtt cgccccacag
      781 ggcgagggcc agcgcgctga gcccggccag ggccacgcga tagcgccaga gcgcgcgcaa
      841 cgccggggtc tgcaacagcc ggggcatggc aggccactgt gccgggatca ggcggcgcat
      901 cacgtcacct ggacaaccac cgcaccggtg atttgctgca acacattggg atcgatgggg
      961 aacagggcgt cgggcgcacc cgcggcggcg tacaccgtgg gccagtcgag cagcgcctgt
     1021 tccatgtaga ccggcagcgc gctcacatgc ccgagcggcg gcaccccgcc gatggcatag
     1081 ccggtcactt cgcgcaccag cgcggcgggc gccatgctga tggccgccgg atcgacccgg
     1141 cgcaggtccg ccagcttcgc cgtgtccacg cgccggtcgc cggggcagag caccagcacc
     1201 gggtcatccc cggccacaaa taccagcgat ttcacaatcg cgccgaccgg gcagcccacc
     1261 gattccgcgg ccaggatcgc ggtcttcgcg ctgcccggca cccggatcac ctggacgccc
     1321 agatccagct cgtccaggac ggcctgtaat cgttcgggtc cactcgccat gcgtccgtct
     1381 ccttggggga aatgtcgctt cagtgtagca cggcgccccg ttgcggtgca agggcggttc
     1441 ccgattgaca cgcgccgcgc cgccgtgcta gactgagccg aaatcctggt gaaagagccg
     1501 ctgccccgcc catgcacact gccgccaata gcgaaccgcc ccgcgccgcc ccggcccgcc
     1561 cgtcgttccc gatcccgtgg gtggacctgc gcaatctcta tatcatcctc gcgggctgcg
     1621 tggccctgct ggtgctgctg ccgcccgagc acgaatatcc gatcatcgac gactggatct
     1681 acgccgggtc ggtccagcat caggtggata cgggcgcctt cgccatgccg ccggagtcgc
     1741 aggccaacct ggtcgggctg acgctctggg gcacgctctg ggcgcggctg ttcggagctg
     1801 gctatactac gctgacctat tccacgctgg cgctggcgct gctggggctg ctggccttct
     1861 atggcctcgc gcgccgggtg ggcacgccgc ccaacgccgc cctgctcggt gtgggactgc
     1921 tggccgtcaa cccgctgttc ctgcacctga gcgccagctt catgaccgac gtgcccttcc
     1981 tggccctggt gctggtcgcg tgctacggct acgtgcgcgg gctgcaatgc gggcagtggg
     2041 gctggctgtt ggtcgcgggg atatgcgccg ggtggggttt cctgatccgc cagttcgcca
     2101 tcctggtgcc ggcgggattc gtgggttatc tgctgctgga agggctgccc agccgccgct
     2161 ggcgccggct tgagatgctg gccgtgatcg ggccgcccgc cctgatcgtg ctgggctggg
     2221 cgatctggag ccgcgacacc gcgacgacgc ccttcaccat ccaggccgac cagcaccgcg
     2281 ccgcctttgt gctccaacca gcctgggtcg aggtcttcct gctgcgcacg ctgaccattc
     2341 tcccgctgac cgcgctctcg gcctgggcgg cgattatcct gccccggcgg cgctggtggc
     2401 tggtcgggct gtgggccgtg ctcgtggccg gggccgaatg gatcgccgcg aacctgtcgc
     2461 acgtctggat cgccaatacg cagccgccgt tcaccttccg tctggggccg ctggcgctcg
     2521 atttccccca gcagacctat ccgttcggct cggagggcag catcttgcgc gagggcggca
     2581 tcgacttctt ccagtaccgc caggagccta tgtggagtcc cgaagcctgg cacctgctgt
     2641 tcgcgctggg gctgatcctg ggcgtccttc tgctggcgaa gatgaccaac agcctgatcg
     2701 actggctgcg cgcctggtgg cgggagcggg accttagccc gctgagcggg gtctacctga
     2761 cgggcctggc gaccttcggc gttacgctgg cgctgggcgg cgacttctac gaccgctacc
     2821 tgctgggctt cgtcgccttc gtcatcctgt tcgtcgtgcg cggggccgcc gggtggagcc
     2881 gccgcgcctg ggcctatacg ctggtcgcct tcgcgctgtg ggccgggttc agcgtgctgc
     2941 tgaaggccga ctccatcgac cacgacaacg cccgctggca ggcggggcaa tggcttgccg
     3001 cccgcgccgg gccggtgcag atgggcttcg actggaacaa cgtccacccg gccggcaacg
     3061 gcacctacga gatcgccgac atgcccatcg ccggcttccg caccgaggcg cggttcccct
     3121 acaccagccg cctgtccggc ttcaccacgc gctacgtgct ggcccaggcg cgcgcggaca
     3181 tgccggcgct gccgccgcca cggcccggcc ggtaggcggc gtgccgccgt tccggttgcc
     3241 gaacggcggg tgacggtgta caatacgccc atgaagattt gcatggtgac gtcctcatac
     3301 ccgaaatatc cgggcgatgt gacggcgcct tttatccagt cgatcgccac ctacgtcgcc
     3361 gcccagggcc atgaggtaca tgtgctggcg ccctggcacc ccgacctgcg ccaccaaccc
     3421 gtcgagaacg gcgtccacct gcatttctat aagtacgccc ctctgcccaa cctgaatatc
     3481 tggggctacg ccgagtcgct ggaggccgat gtgcgggtgc gcaacgcgat atacccgctc
     3541 acgcccctgg tcacgctgat cagttttttc gctctgatgc gcctgaccgg gcgcattaag
     3601 tttgacgtgc tgcaagccca ctgggtgatc ccgaacgccc cggtggccgc ggtggtggcc
     3661 cgcctgcgcg gcctgcccct ggtgatcagc ctgcacggct cggatgtgtt tgtcgcctcg
     3721 cagaaccgcg tgctggcctg ggcggcccgc cagtgtttcc gcgccgccga cgccgtgacg
     3781 gcgtgcagcg aggacctgcg cgagcgcgcg ctggcgctcg gcgcggcgcc ccggcgcacc
     3841 cggttggtgc cctacggcgt cgatccctgc gcttttgccc ccatcgaaga cgcgggcgcc
     3901 gacctgcgcg aacggctggg gctggcgccg ggcacacccg tcgtcctggc aatcgggcgc
     3961 ctggtctaca agaagggctt cgagtatctg atccaggccg ccccgaccat cctggcccgc
     4021 cacccccagg cccagatcta tatcgccggg cagggcgatc tgcagcgcga actccaggcg
     4081 caagccgccc gcgtcggcgt ggccggccat gtccacctgc cgggcaaggt cagccatgac
     4141 gacgtgccca cctacctcag cgggtgcgat gtcttcgtgc tgccctccgt ggtcgaccag
     4201 agcggcaatg tcgatggcct gcctaatgcg ttactggagg cgatggccgc gggctgcgcc
     4261 gtggtcgcca gcaacgtggc cggggtgccg ctggccgtgc agagcggcga gaacgggctg
     4321 ctggtgccgc aaaaagatcc gggccggctg gccgaggcgg tcagcaccct gctgggcgat
     4381 gccgcgctgc gcgccgagat gggcgccgcc gcccgccgca agatcgagca ggaactgaac
     4441 tggccgcaca ttgcccgcat gttcgagagc atctatcgtg tggcgagcgc gcggcgcacc
     4501 cgg
//
LOCUS       DASUYC010000970         2618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_856535,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000970 DASUYC010000000
VERSION     DASUYC010000970.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856535"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="VF276_15885"
     CDS             <1..893
                     /locus_tag="VF276_15885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608382.1"
                     /translation="SRTLLISVLLVLFGLTVLLVARSLPPPAVPPAGGDPQPPRVVVI
                     AVTATPVDTPTPMPTPTASSTPSPSVTAPPAAPPPAATKAPPSMHVALPRPTRLPPSA
                     TPTPHPVVHPAERTVRPSVEPRHATEPPAPPPPAPTPTAAAAAPASFAPSPGPAAPGP
                     PSPTVGVTLSPTPTAARTVLPPPGKPPTRPPAPTATPLPPTAPPLVLTATPTPAAMDP
                     TNTPLPPTATAGPDTPTPEATVTPAPSPATPPSPTDTPVDTTATPAPPTATPAPITPP
                     ATVVPSATPVPPTVTPPGPR"
     gene            complement(919..1575)
                     /locus_tag="VF276_15890"
     CDS             complement(919..1575)
                     /locus_tag="VF276_15890"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX6608383.1"
                     /translation="MENGDGRRRRSAPDMRREQILDGAEQALLDLGWAGTTFSTIAAR
                     SGVARSALNYFFADKQAIMQALVARLFERATASIDQLTSLPVRDPVRVLLSIRKRLMA
                     PTADELRMVHLLLTCWSAGLHDPAIRHQLTGYFQFFRGRLELVLAAGSAQGVLRVEDP
                     AAAAGWLLAGIHGLLLQHLYIPNRELTRRAAVGLFGQLGLETPAVDGGNQALSAAEQA
                     "
     gene            1729..2355
                     /locus_tag="VF276_15895"
     CDS             1729..2355
                     /locus_tag="VF276_15895"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX6608384.1"
                     /translation="MEHQPNSRTQRTRERLRAAAHRLFLQQGYLATSVDAILAEAGIS
                     SKETLYRHYANKEALFVDVLSHLTMEQPGFSEKLAALPPPHDLASLRQALTQLSHEIV
                     SMMSQPGYLPLVRMVLAEEPRFPQLGSLFFATVTQRGIAIIMGLLLAARERQLIADID
                     LAAVTHTLLGGLVTSVLLSHVLAKEETQSSAYSRADGVVEVIMRALTP"
     gene            2415..>2618
                     /locus_tag="VF276_15900"
     CDS             2415..>2618
                     /locus_tag="VF276_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX6608385.1"
                     /translation="MNKDNKLKRNFAKRFVNPLLHNLGRASFGPFALLRHVGRRSGKT
                     YEIPIMVWPVQDGFLIALTYGRQV"
BASE COUNT          431 a          965 c          783 g          439 t
ORIGIN      
        1 acagccggac cctgttgatc agcgtgttgc tcgtgctctt tggcctgacg gtgctattgg
       61 tggcgcgcag cctgcccccg cccgccgtgc cgcccgcggg gggcgatccg cagccgccgc
      121 gtgttgtcgt gatcgccgtc acggcgacgc cggtggacac cccgacgccg atgcccacgc
      181 caaccgcgtc ctcgactccg tcgccatcgg taacagcgcc gccagcagct ccccctccgg
      241 cggcgacgaa agcgccgccc agcatgcacg tggcgctgcc acgtcctacc cggttgccgc
      301 cgtcggccac acccacgccg catcctgtgg ttcatcccgc cgaacgcact gtccggccca
      361 gcgtcgaacc gcgccacgcc accgagcctc cggccccgcc gccgcccgcg cccaccccta
      421 cggcagcggc agccgcgccc gcatccttcg cgccctcacc ggggccggca gcgcccggcc
      481 cgccgtcgcc cacggttggc gtaaccctgt cacccacgcc gacggctgcg cgcacggtgc
      541 taccgccgcc cggcaaaccg ccgacccgcc cgcccgctcc gaccgcgacg ccgctcccgc
      601 cgaccgcgcc gcccctcgtg ctcacggcca cgccgacacc cgcggcgatg gatccgacca
      661 acacgccgct gccgccgacc gcgacagccg ggccggatac gccgaccccg gaagccactg
      721 tcacgcccgc cccgtccccg gccacgccgc cctcgccgac cgatacgccg gtcgatacga
      781 cggccacgcc cgccccgccc acggccacgc ccgcgccgat cacgccgccg gccacggtcg
      841 tgccctccgc gacgcccgtt ccgcccaccg ttacgccgcc cggcccccgc taagcccagg
      901 ggctgctgcc cggcggcgct acgcctgttc cgcggcgctc agcgcctgat tcccaccgtc
      961 gacggcgggc gtctctaacc ctagctggcc gaataagccg acggcggccc ggcgcgtgag
     1021 ttcccggttg ggaatgtaga ggtgttgcag cagcaggccg tggatgccgg ccagcagcca
     1081 cccggccgcg gctgccggat cctcgactcg tagtacgccc tgtgcgctgc ccgctgccag
     1141 cacgagttcc aaccgcccgc ggaaaaactg gaaatagccc gtcaactggt ggcggatcgc
     1201 cgggtcgtgc aaacccgcac tccagcaggt tagcagcagg tggaccatgc gcaactcatc
     1261 ggcggtgggc gccatcaggc gcttgcggat gctcaacagg acgcgcaccg ggtcgcgcac
     1321 cggcaggctg gtgagctgat cgatgcttgc ggtggcccgc tcgaacagtc gcgccaccag
     1381 cgcctgcatg atcgcctgtt tgtcggcgaa gaaatagttc agcgccgagc gcgccacccc
     1441 ggagcgggca gcgatcgtgc tgaaagtggt gcccgcccag ccgaggtcca gcagcgcttg
     1501 ctccgcgccg tccagaatct gctcgcgccg catgtccggc gcgctacgcc gccgccgccc
     1561 gtcgccgttt tccataggtg ctccttttct agtacgattg tacaatatga accagcacca
     1621 tgcaaggatc caatgatcta ttgccgcctt cgtcatactg attgacaagt cacgtggtct
     1681 gtactataat cgtaacgtat agtacgttac gattggagag acgctccaat ggaacatcag
     1741 ccgaacagcc gtacccaacg aacacgcgag cggttgcgcg cagccgccca ccggctgttt
     1801 cttcaacagg gctacctggc aacaagcgtc gatgccatcc tggcagaggc tggtatctcc
     1861 tcgaaggaga cgctctaccg gcactatgcc aacaaggagg ccctcttcgt cgatgtcttg
     1921 agccatctga caatggagca gccaggcttt tcggagaagc tggcggctct gcccccgccg
     1981 catgatctgg cgtcgctgcg ccaggcactg acgcagcttt cgcatgagat cgtctcgatg
     2041 atgagccagc ctggctatct gcccctggtg cgcatggtcc tcgctgaaga gccgcgcttt
     2101 ccgcagctcg gctctttgtt tttcgcaacc gtcacgcagc gcgggatcgc gatcatcatg
     2161 ggcttgctgc tcgccgccag ggagcgacag ttaatcgccg acattgatct ggcggcggtg
     2221 acacacacgt tgctcggcgg gttggtgacc tcggtactgt tgagccacgt gcttgcaaaa
     2281 gaggaaacgc aatcttcggc atatagccgt gccgatgggg tcgtcgaggt tatcatgcgc
     2341 gcattgaccc cgtaagcgtt gcttttggcc tgtgttcgtt gcataccgga gggtcatata
     2401 caaggagtaa caacatgaac aaggacaaca agctcaaacg gaactttgcc aagcgtttcg
     2461 tcaatccgct cttgcataat ctggggcgcg cttcctttgg cccgttcgcg ctgttgcgtc
     2521 acgtgggccg gcgcagcgga aagacctacg agataccgat catggtctgg ccggtacagg
     2581 atggcttttt gatcgcgttg acctatggcc ggcaggtc
//
LOCUS       DASUYC010000971         2791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_623909,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000971 DASUYC010000000
VERSION     DASUYC010000971.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623909"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VF276_15905"
     CDS             complement(<1..656)
                     /locus_tag="VF276_15905"
                     /inference="COORDINATES: protein motif:HMM:NF021059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase"
                     /protein_id="HEX6608386.1"
                     /translation="MITTLHPGIATVLAAVRDYGYDTNAYHALGDIYAARQGELLLFN
                     YTARAAYARRWNDVERVCRGLIVHVPTATVRALPFEKFFNLGETLDTTLEALPAGPCE
                     ITAKMDGSMAILYRADDGPAIATRGSFASSQAQWATAHLRARYDLRDLPDDVTLLFEI
                     IYPENRIVVNYGALEALVLIGARRLDGYDYTYPELAAVAARYGFPLVAREPALRIHDV
                     V"
     gene            complement(782..1372)
                     /locus_tag="VF276_15910"
     CDS             complement(782..1372)
                     /locus_tag="VF276_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018584404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX6608387.1"
                     /translation="MEFSEVVRKRRMVRNFTDEPVAPEVVARMLELARHAPSAGFTQG
                     QSFVVVTRPELKHEIARICGEESYVESGFHPWISGAPVLVIACTSEAAYHRRYQEPDK
                     VRDDGSEIEWPVPYWHMDIGCAVMILLLAAVDEGLAAGFAGAFDLAALRAVLGIPPEV
                     TPVGVIPIGHPAPDRRSPSLKRGRTADADYVHHEGW"
     gene            complement(1527..>2791)
                     /locus_tag="VF276_15915"
     CDS             complement(1527..>2791)
                     /locus_tag="VF276_15915"
                     /inference="COORDINATES: protein motif:HMM:NF024298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX6608388.1"
                     /translation="LLSLLGNFRYQQKYPNGAPGQSTATAPGTVSFPQTGKHLGGRFL
                     TYWQQHGGLAQQGYPISEEFMERSELDGKLYKVQYFERAVFEYHLENQPPYDVLLSQL
                     GKFRYEARGETPPPATGAISAASAARVALTGKLAGHASTVTGVAFAPDGQTVASASQD
                     GTVKLWRAADGKVLRTLQGADGMTSVAFAPDGQTLAAGSKDKTVRLWRVSDGQPVRTL
                     AGHSYMVQSVAYSRDGQTLASGSLDGTVKLWRAADGALLRTLDGGGSSINEVAFAPDG
                     ATLAAALGNKTVRLWHVADGAALRTLIGHTDIVHSVAFAPDGQTLASAAEDGTIRLWR
                     VADGAALRTLAGHSDGVYAVAFSPDGSLLASGSYDETLRLWRAADGAALRTLEGHKGS
                     IQSVAFAPNGATLATASSDDTVGLWTAR"
BASE COUNT          408 a          984 c          970 g          429 t
ORIGIN      
        1 acgacatcgt ggatgcgcag cgccggctcg cgcgccacca acgggaagcc gtagcgcgcc
       61 gcgaccgcgg ccagttcggg ataggtgtag tcgtagccgt ccaggcggcg cgccccgatc
      121 aacaccagcg cttcgagcgc gccgtagttg acgacaatgc ggttttctgg gtagatgatc
      181 tcgaacagca aggtcacgtc gtccggcaga tcgcgcaggt cgtagcgcgc ccgcaggtgg
      241 gccgtggccc actgggcctg gctgctggcg aagctgcccc gcgtggcgat ggccggaccg
      301 tcgtccgccc ggtagaggat cgccatgctg ccgtccatct tggccgtgat ctcgcacggc
      361 ccggcgggca gcgcctccag cgtcgtgtcc agcgtctcgc ccaggttgaa gaacttctcg
      421 aagggcaggg cgcgcacggt ggccgtgggc acgtggacga tcaggccgcg gcagacgcgt
      481 tccacgtcgt tccagcggcg ggcgtaggcg gcgcgggcgg tgtagttgaa cagcagcaac
      541 tcgccctgcc gcgcggcata gatgtcgccc agcgcgtggt aggcgttggt gtcatagccg
      601 tagtcgcgga cggccgccag cacggtggcg ataccggggt gcaacgtggt gatcatcgac
      661 aagggccctt tcctcccgcg tggtttcttc ctcattatag ggatgccggc gcgggcgaca
      721 agggaccgcg gaacgggacg cgcctccatc gggcgatgga atcgcgtccc gccgcccggt
      781 cctaccagcc ctcgtggtgg acgtagtcgg catccgccgt gcggcccctc ttgagcgatg
      841 gcgaccgccg gtccggcgcc gggtggccga tggggatcac gccgaccggg gtgacttccg
      901 gcgggatgcc cagcacggca cgcagcgcgg cgagatcgaa tgcgccggca aagcccgccg
      961 ccaggccctc gtccacggcg gccagcagca gaatcatcac ggcgcagccg atgtccatat
     1021 gccagtaggg caccggccat tcgatctcgc tgccgtcgtc gcgaaccttg tccggttcct
     1081 ggtagcgccg gtggtaggcc gcctcgctgg tgcaggcgat caccagcacc ggggcgcccg
     1141 aaatccaggg gtggaacccg ctctcgacgt agctttcctc gccgcagatg cgcgcgatct
     1201 catgcttcag ctccgggcgc gtgacgacca cgaacgattg gccctgggtg aacccggcgc
     1261 tcggcgcgtg gcgggccagt tcgagcatcc gcgcgaccac ctccggcgcg accggttcat
     1321 cggtaaagtt gcggaccatg cgccgcttgc gcaccacctc actaaactcc atcgtctcct
     1381 cctcctgcag cgggccgggc gcccaggccg ccgggtcgcg ccggccctgc acggcacccg
     1441 gcaataagag gagcgaggcg tggtgacctc gctcccgtat ttacgctcta tactatatac
     1501 cgccggtcag gccgcgcgca agcaggtcag cgcgccgtcc acagccccac ggtgtcatcc
     1561 gacgaagcgg tggcgagcgt ggcgccgttg ggcgcgaagg ccacgctctg gatgctgccc
     1621 ttgtgccctt ccagggtgcg cagcgccgcg ccatcggcgg cccgccacag gcgcaacgtc
     1681 tcgtcatagc tgccggatgc cagcaggctg ccgtcgggcg aaaaagccac cgcatagacg
     1741 ccgtcgctat gcccggccag ggtgcgcagc gcggcgccgt cggccacccg ccacagccgg
     1801 atcgtgccgt cttccgccgc cgaggccagc gtctggccgt cgggcgcgaa ggccacgctg
     1861 tggacgatgt cggtgtggcc gatcagcgtg cgcagggcgg cgccgtcggc cacgtgccac
     1921 agccggacgg ttttgttgcc cagcgcggcg gccagggtcg cgccgtcggg ggcaaacgcg
     1981 acctcgttga tcgaggagcc gcccccatcg agcgtgcgca gcagcgcgcc atccgccgcc
     2041 cgccacagct tcaccgtgcc gtcgagcgac ccggaggcca gcgtctggcc gtcgcggctg
     2101 taggccacgc tctgcaccat gtagctatgg ccggccaggg tgcgcaccgg ctgcccgtcg
     2161 ctcacgcgcc agagccgcac cgtcttatcc ttgctgccgg cggccagcgt ttgcccatcg
     2221 ggggcgaagg ccacgctagt catgccgtcc gcgccttgca gggtgcgcag gaccttgccg
     2281 tccgccgcgc gccacagttt caccgtgccg tcttgcgagg ccgaggcaac cgtctgtcca
     2341 tcgggggcga aggccacgcc ggtgacggtc gacgcatggc ccgccagctt gccggtcagg
     2401 gcgacgcgcg cggcgctggc cgccgagatg gcgcctgtcg cgggcggcgg cgtctcgccg
     2461 cgcgcctcgt agcggaactt gcccaactgg ctcagcagca catcgtacgg cggctggttc
     2521 tcgaggtggt actcgaacac ggcccgctcg aaatactgca ccttgtagag cttgccgtcc
     2581 agctccgacc gctccatgaa ctcttcggag atgggatagc cctgctgcgc caacccgcca
     2641 tgctgctgcc agtaggtcag gaaccgcccg cccaggtgtt tgccggtctg cggaaacgac
     2701 acggtgccgg gcgcggtggc cgtgctctgg ccgggcgcgc cgttgggata tttctgctgg
     2761 tagcggaagt tgcccagcaa cgacagcagc a
//
LOCUS       DASUYC010000972         2391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1316177, whole genome shotgun sequence.
ACCESSION   DASUYC010000972 DASUYC010000000
VERSION     DASUYC010000972.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1316177"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..585)
                     /locus_tag="VF276_15920"
     CDS             complement(184..585)
                     /locus_tag="VF276_15920"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEX6608389.1"
                     /translation="MTSRRALGRQGEALAAAYLQAQGLVIRDANWRPTGTDLAPLLRG
                     ELDLVAMDGDTLVFIEVRSRRGAPGLAEESVTPAKRRQLLALARAYLATHDLHEDQVP
                     WRVDVVALHFTAASAPGINWLPGAVTAESGW"
     gene            complement(582..1322)
                     /locus_tag="VF276_15925"
     CDS             complement(582..1322)
                     /locus_tag="VF276_15925"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX6608390.1"
                     /translation="MPRTWKPPAGVSPAPPGPLQPDWNEEAAALAAGYRAVAGCDEVG
                     RGALAGPLMAAAVVFPSALIAAAATGAADPSLQDCLDELAAVRDSKQLTPAARCRLDA
                     CIRRHGQVGLGLVSAALCDQIGMGAANRLALTRAIRALPGPVEYVLVDACRLPRLPLP
                     HLALIKGDSRSVSVAAASIVAKVARDALMDGYHTCWPAYCFDENKGYGTPAHLRTLIQ
                     HGPTPQHRRAFDPLRSLLAAPDSPAPVP"
     gene            complement(1398..1751)
                     /gene="rplS"
                     /locus_tag="VF276_15930"
     CDS             complement(1398..1751)
                     /gene="rplS"
                     /locus_tag="VF276_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010093457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX6608391.1"
                     /translation="MNELLRSVTQSQLRTDIPEFGSGDTVRVHAKVVEGTRERIQVFE
                     GVVMRKRAGGIEANFTVRRVASHGIGVERTFMINSPRIDKIEVVRRGLVRRAQLYYLR
                     KRTGKAARIKEKRFV"
     gene            2039..>2391
                     /locus_tag="VF276_15935"
     CDS             2039..>2391
                     /locus_tag="VF276_15935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608392.1"
                     /translation="MPTPREPGAPDEWETPGPDIPPDVWRDVRRLSTPPPGADPYVLA
                     PPPPATAGRPVRAAVGRTTDAVLAGLAHGAIIFGFFGVGFLLSLAFNLVLWLYSRRSP
                     YVAYHAQQAGCYQCFV"
BASE COUNT          377 a          809 c          842 g          363 t
ORIGIN      
        1 ctggccggca tgctcaggga gtaaaacgcg ggccgcggcg gggaatgacg gcggcaaggc
       61 acaaaaaagg cacaaaatcg ggggccaccg gaaccgcctg attcttcctt tctagtgccg
      121 aaaattatat cgatccgcac cctggttgtc aatcatttgc gggtctggtt cgcgcggggg
      181 gatttaccag ccggattccg cggtgacggc gccgggcagc cagttgatgc ccggcgcgga
      241 tgcggcggta aagtgcaggg ccaccacatc gacgcgccag ggcacctggt cctcgtgcag
      301 gtcgtgcgtg gcaaggtagg cacgggccag cgccagcagt tggcggcgct tggccggggt
      361 gacggactct tcggccaggc cgggcgcgcc ccggcggctg cgcacctcga taaacaccag
      421 tgtgtcgccg tccatcgcca cgagatcgag ttccccgcgc agcagcgggg ccaggtcggt
      481 gccggtgggg cgccagttgg cgtcgcggat gaccaggccc tgggcctgga gataggcggc
      541 ggccagcgcc tcgccctggc ggcccaacgc gcggcggctg gtcatgggac gggcgccggc
      601 gagtctggcg cggcgagcag gctgcgcagc gggtcgaagg cgcggcggtg ctgcggagtg
      661 gggccgtgct ggatgagcgt gcgcagatgg gcgggggtgc cgtagccctt gttttcgtcg
      721 aagcagtagg cgggccagca ggtgtggtag ccgtccatca gagcgtcacg ggccaccttg
      781 gcgacgatgg acgccgcggc gacgctgacc gaccgtgagt cgcctttgat gagggccagg
      841 tggggcaggg gcagccgggg caggcggcag gcatcgacga gcacatactc gaccggcccc
      901 ggcagggcgc ggatggcgcg ggtcagcgcc aggcggttcg ccgcgcccat gccgatctga
      961 tcgcagagcg ccgccgagac cagccccagg cccacctgcc cgtgccggcg aatacaggcg
     1021 tctagccggc agcgggcggc gggcgtgagt tgcttggagt cgcgcacggc ggccagttcg
     1081 tccaggcagt cttgcaggga cggatcggcg gcgcccgtcg cggcggcggc aatcagcgcg
     1141 gaggggaaga ccaccgccgc ggccatgagc ggcccggcca gcgcgccccg ccccacctcg
     1201 tcgcacccgg ccacggcgcg atacccggcg gccagggccg cggcttcctc attccagtcg
     1261 ggttgcagcg ggccgggcgg cgcgggcgag acgccggcgg gcggtttcca cgtgcgcggc
     1321 atgcgccgat cctccgcaaa tgcgtcgcgc gcgaccccca ggccgcgcgc ggacagaaca
     1381 gattaggcca gcgtgcgcta gacgaagcgc ttttccttga tgcgcgccgc cttgccggtc
     1441 cgcttgcgca gatagtacaa ctgcgcccgg cgcaccaggc cccggcggac gacctcgatc
     1501 ttgtcgatac gcggcgagtt gatcataaac gtgcgctcga cgccgatccc gtgcgacgcg
     1561 acgcggcgga ccgtgaaatt cgcttcgatg ccgcccgccc gcttgcgcat gaccacgcct
     1621 tcaaagacct ggatacgctc cctggtgcct tcgacgacct tagcatgcac acgcacagta
     1681 tcgcccgacc cgaactccgg aatatcggtg cgcaactgcg actgcgtcac actccttaat
     1741 aattcgttca tgccctggac tcctataatt aatatgctcg ctaaggcgcc ggcaaagggc
     1801 gcgacgttta gtataccata ctcgttccaa aggtgcaagt gatatgcggc ccggaaacca
     1861 cgcgcatccg cggttcagta gcggcgattt tgcgtaatcg ccggcacgcc gccgcccgga
     1921 tgaaatgccc tggacgtgtc ttgcgtatac attccagtct agtataccaa attgccggcg
     1981 gccagggcgc gatgcttacg cgccggcgac cggcatcacg cgaacaggga gacctaccat
     2041 gccaaccccc agggaaccgg gcgcgccgga cgagtgggag acaccggggc cggacatccc
     2101 gcccgatgtg tggcgcgacg tgcgccgcct gtccacgccg ccgcccggcg cggacccgta
     2161 tgtgctggcc ccgccgccgc ccgcaacagc cgggcgcccg gtgcgcgccg ccgtgggccg
     2221 caccaccgac gcggtgctgg ccgggctggc ccacggtgcg attatcttcg gcttcttcgg
     2281 cgtcggcttc ctgctgtcgc tggcctttaa cctggtgctc tggctctact cccggcgctc
     2341 gccctacgtc gcctatcacg cgcaacaggc cgggtgctac cagtgctttg t
//
LOCUS       DASUYC010000973         2559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1960877, whole genome shotgun sequence.
ACCESSION   DASUYC010000973 DASUYC010000000
VERSION     DASUYC010000973.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1960877"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VF276_15940"
     CDS             complement(<1..351)
                     /locus_tag="VF276_15940"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX6608393.1"
                     /translation="MTVQSTNSTAAPAALGYGEVQTPVADLRAEPRDDAERVNQALLA
                     TPLEVLDTHTDAAGDAWLRVRLPDYTGWMRAEAITRLDAPPTPGRQVRVTVPRTTLRI
                     LDDSGAASGETVDAF"
     gene            complement(348..1190)
                     /locus_tag="VF276_15945"
     CDS             complement(348..1190)
                     /locus_tag="VF276_15945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEX6608394.1"
                     /translation="MPASPSRIQAPAAGPPQGGARRRRLNILGVRIDDSPLGESVARC
                     ARMLAGAPAAGGGRLHQVTTVNPEFVMRARQDAEFRAVLNRADLATPDGAGIILGSLI
                     LGRRLRGRTTGVALTEELAALAARAGYRLFLLGAQPGVAAEAGRIWMSRYPGLQVAGA
                     YPGSPHEAEREEITARITAAEADLLFVAFGAPAQDLWIARNRAALPTVRLAMGVGGVF
                     DYVTGRVPLAPPLLRRAGLEWLYRLYKQPWRWRRMLALPAFALAVIGQRLGLVRLPAS
                     RGNA"
     gene            complement(1449..1964)
                     /locus_tag="VF276_15950"
     CDS             complement(1449..1964)
                     /locus_tag="VF276_15950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608395.1"
                     /translation="MKKLIIILGVFVLFAGITVAGAASNPLSLHQGPVPPTPIGATIP
                     STGPVKQRPLPQTPHDPAAWGAPAITPPVTEARVRDYFAQRPPVFPGMSPLQPPTLTA
                     VEFMTVAQLRQRLGVPNDLSYYSGTAQIVYVSFTGKVGVDQADPNKPQAPYGQTYMVF
                     DATTGNVLIGP"
     gene            2251..>2559
                     /locus_tag="VF276_15955"
     CDS             2251..>2559
                     /locus_tag="VF276_15955"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX6608396.1"
                     /translation="MQIIIPAGGLGTRMRPHTYSKPKPLVAVGGKPSIAHILDAFAAL
                     PIDKVVLITGRQGEMLLDYVKEHYGFEAEAVEQKVMRGQSDAILLAEPMIDKAEDLFV
                     V"
BASE COUNT          459 a          851 c          839 g          410 t
ORIGIN      
        1 gaaggcgtcc acggtctccc cgctcgctgc acccgagtcg tcaaggattc gtagggtcgt
       61 gcgcgggacc gtcacgcgca cctggcggcc cggcgtgggc ggcgcatcca gccgggtgat
      121 ggcttcggcg cgcatccaac cggtgtagtc gggcaggcgc acgcgcagcc aggcgtcgcc
      181 cgccgcgtcg gtgtgcgtgt ccagcacttc cagcggcgtc gccagcagcg cctggttcac
      241 ccgttcggcg tcgtcgcgcg gctcggcgcg cagatcggcc accggcgtct gcacttcgcc
      301 atagcccagg gccgcgggcg cggcggtcga attcgtcgat tgcacggtca tgcgtttcct
      361 ctcgaagccg ggagccggac cagacccagc cgctggccga tcaccgccag ggcaaaggcc
      421 ggcagcgcca gcatccggcg ccagcgccag ggttgcttgt agagccgata gagccattcc
      481 agccccgccc gccgcaacag cggaggcgct aagggcaccc ggccggtcac ataatcaaag
      541 acaccaccca cgcccatcgc caggcgcacc gtgggcaacg cggcgcggtt gcgcgcgatc
      601 cagaggtcct gggccggggc gccaaaggcc acgaacagca gatcggcctc cgccgcagta
      661 atccgcgcgg tgatttcctc gcgctcggcc tcgtgcggcg agccgggata ggcgcccgcc
      721 acctgcaagc ccggatagcg gctcatccag atgcggcccg cctcggcggc cacgcccggc
      781 tgcgcgccca gcaggaacag gcgatagccc gcccgcgccg ccagcgccgc cagttcttcg
      841 gtcagcgcga cgccggtggt gcgcccgcgc agccgccgcc ccaggatcaa cgagccgaga
      901 atgatcccgg cgccgtcggg cgtggcgagg tcggcccggt tgagcacggc gcggaattcg
      961 gcatcctggc gggcgcgcat cacaaactcc gggttcaccg tagtgacctg gtgcaggcgc
     1021 ccgccgcccg ccgccggggc gccggccagc atgcgcgcgc agcgcgccac cgactcgccc
     1081 agcggactgt cgtcgatgcg cacgcccagg atattcaagc gccggcgccg tgccccgccc
     1141 tggggcgggc cggctgccgg cgcctggatt cggctagggg aagccggcat cgctgctcct
     1201 tctccagaca caaactaccc cacccacagg cagggcaggt ttcattatag tatacgccta
     1261 tcttttttgc caggatgcgt gacggcgatc ccgccggtgc gggtcgcagg gtacttaagc
     1321 cgaaggttcg gaggcagaag gggcaaactc tatattctca tagatcgccg ttagcggcag
     1381 cacagcgtca atcgacggtt gtggcgtcga agaccatgta cgcccgccca taggcggcgt
     1441 ccggcgtatt acggtccgat gagcacattg cccgttgtgg cgtcgaacac catatatgtc
     1501 tgcccatagg gtgcttgtgg cttattggga tccgcttgat ctacaccgac cttcccggta
     1561 aacgatacat acacgatctg tgccgtaccg gaataataac tcaggtcgtt gggaacaccc
     1621 agccgctggc ggagctgggc cacagtcatg aactccacag cggtgagcgt gggcggttgc
     1681 agcggggaca taccggggaa gaccggtggg cgttgggcaa aatagtcgcg cacgcgggcc
     1741 tcggtcacgg gaggcgtaat ggccggcgcc ccccaggcag ccggatcgtg tggtgtttga
     1801 ggaagaggcc gctgcttaac cgggccggtg gatgggatcg tagctccgat aggtgtcggt
     1861 gggacaggac cttgatggag cgacaacggg ttgctggcgg cgcctgctac ggtaatccca
     1921 gcgaagagca cgaacactcc aagaatgatg atcaacttct tcacgacggt tctccttaat
     1981 gatcccaaat gatccccatc ggaatcctga agagcggcag gccggtttga aaccggcccc
     2041 tacagaggaa aataacagtg gccgcctgtt attctacact cgtctatatc acagtatact
     2101 cgctcccgca atatctaccg ccagggccgg ttagtaggca gcgcccttgc acgagggcac
     2161 acttttcgct acactggccg tacagatacc ggctgcgcgc cggttggcgt cccccacggg
     2221 ggcagagaga ccctatccac gaggagcgct atgcagatca ttatccccgc cgggggcctg
     2281 ggcacccgta tgcgaccgca cacctacagc aagcccaagc cgctggtcgc cgtgggcggc
     2341 aagccgtcca tcgcccatat cctcgacgcc tttgccgccc tgcccatcga caaggtggtg
     2401 ctgatcacgg ggcgccaggg cgagatgttg ctggattatg tcaaggaaca ctatggcttc
     2461 gaggccgagg ccgtggagca gaaagtgatg cgcggccaat cggatgccat cttgctggcc
     2521 gagccgatga tcgacaaggc ggaagacctg ttcgtggtc
//
LOCUS       DASUYC010000974         4562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1167091, whole genome shotgun sequence.
ACCESSION   DASUYC010000974 DASUYC010000000
VERSION     DASUYC010000974.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4562
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167091"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(654..776)
                     /locus_tag="VF276_15960"
                     /pseudo
     CDS             complement(654..776)
                     /locus_tag="VF276_15960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
     gene            1471..2622
                     /gene="lysS"
                     /locus_tag="VF276_15965"
     CDS             1471..2622
                     /gene="lysS"
                     /locus_tag="VF276_15965"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HEX6608397.1"
                     /translation="MCPTLRVQIVDSTLREGEQFAGAHFTSAARVAIAEALDAFGVEY
                     IELTSPVASPQSAQDLRTIAALPLRARVLTHTRCRLPDVQLAADCGVDGVNLLFGTSP
                     YLRAYSHGRSLDAILAETTTVIRFLQERGIEVRFSCEDAFRTPLADLLRVYRAVDALG
                     VQRVGIADTVGIATPRQVTAVVAAVRAAVGCDIEFHGHNDGGCAIANADAALHGGATH
                     IDTTVLGIGERNGIAALSGLIARLYLTDPALVAHYHLPLLPDLDAMVARMVGVEIPFN
                     ACITGATAFTHKAGMHTHAVLQEPRAYEALDPAAFGRERAVLMGHRLTGRHALAHRAA
                     GLGLQLSDDALRATTDQVKALADAGPLTQVQVDALLHEHAAPDGMAPNA"
     gene            2654..3901
                     /locus_tag="VF276_15970"
     CDS             2654..3901
                     /locus_tag="VF276_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016330125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX6608398.1"
                     /translation="MAQTLAEQILSHAAGRPVRAGEVHTVAVDLVMVHDSIAPSVIRV
                     LQDELGCARVFDADRVAVVIDHVSPAANVQTAEAQRRLRAWVREQGIAHWFESGRGIA
                     HQVLIEERLARPGRLITGSDSHSTAYGAVAAFGTGMGATDIALALATGHTWLRVPETI
                     LVRARGNFGSGVGPKDLALWLARTLGADGATYRAVEYHGLDFLTVGDRTTLTTMALEV
                     GAKAGIVAPAGLPDRYPVPDWLRVEDGARYERIIEVDLDALSPQIAVPNTVDNVVDLR
                     DLGRVAVDVVYLGTCTNGRHADMAAAARILQGRHVAPGVRMMVVPASSEALQEAIADG
                     TLTTLLSAGATIGTPGCGACIGRHMGVLAPGEICLFTGNRNFRGRMGAPEAGIYLASP
                     EVAAATAVAGYIADPRELDALPA"
     gene            3935..4492
                     /gene="leuD"
                     /locus_tag="VF276_15975"
     CDS             3935..4492
                     /gene="leuD"
                     /locus_tag="VF276_15975"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF002458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX6608399.1"
                     /translation="MSRVWRFGDDVNTDQIVPGRYAPYMTSEAELRKYPFIEARPEFA
                     ATVQPGDLIVAGRNFGCGSSREHAPWALMDFGIRCVVSTSFADIFYNNCFQNGLLPVV
                     LKADEVQQLMDEARGGNHVVSVDLEAQTVTSPSGAVFGFDIDPKRKAKMLGGLDAIGE
                     TLTRAGDIDVFEMKRALSQPWLEEA"
BASE COUNT          806 a         1560 c         1454 g          742 t
ORIGIN      
        1 accgcgctgc tgccggcact ggcagcgaga gggatgcgcg ccacaactct gtggtcgcgc
       61 gcggaggatg cgttagcggg ttggccgccg caccggcacc ggcaccagga ccggggtccg
      121 gggcgccaac aggctctcta aggctttttt gatgcgatcc agcatatacg tcactttcgc
      181 tgtgcgggcc ttaatagccg gcccctctga ttttgcggtg taggggccgg tttcaaaccg
      241 gcctgtcccg gataatcaaa aagcccctcc cggttgggga ggggcctgac ttactgaacc
      301 cgtttgttac gctgcctgcc tccccaacgc cgattgaccc ttaacaataa taagggcaat
      361 aaggaggagg ataaggatga agttggcagc gactcgcgac ataactgtgt tccttgtcgt
      421 aaggctcggt taacttatgc cagagtataa aggatttctt acacgctgtc aataggttcc
      481 ggcagggcga aacctcgaat cccgatacaa acgcagcaat tatcgctaaa actagccgta
      541 aaataaatgt gcaattcgtc acaaattcat ctgcgccgca tgccggcggc ccggtgggca
      601 gtacccgcct attagatcgc ttgccgttgg cggggttatc agatcgcgcg acggacgcag
      661 atatcacaaa taaagacccc atgcggcccc gcaatcagcc gcgcaacatc cgtctgtcgc
      721 cggccacaaa aggagcagtg aagccgagcg ggtgtagcag gctccccatg cgggatgatt
      781 cgctgcgtta tcctgacagc cgccgcatca gcgtgcggcg taggatcgag aatctgctca
      841 caggtcgcga cgcacgcggg acagatggcg atgccgcccg gtcctgtata gagcgtgtct
      901 gtatctgaga ccgcccgatg acaaaatgag caggtttgac cggtggtgct ggacatagga
      961 tcattccgtc tttctgaggc cgcgaacagc aaaagcgtaa gccattatat gccgggttgt
     1021 cgctttccgt caaaatccaa attctgcgta acagagctaa tgcatgcatg gaaagcgaga
     1081 gacgcatctc gacgttgcca agatatgccg ggcaaaccta ttgacaagcc gcgcaacttt
     1141 tgctaggata ggccccatca accgttcgcg gaaggatcag ggcatgtcgt caatgcaccg
     1201 ccaactgcat atcagcgcca acaagcagga caagcgctcc aagaagccgg ccgagaggtc
     1261 tggatagtgg cgtttgtgtg ctaagctgcc caacggatgc ccgccggacc tcgtgaggtc
     1321 cggcggtttt tatttgtccc aaatttagta cctgtggagg aaacaccatg aagagcctga
     1381 agaagcaact gaagtccaag cgcatgttcg tcacctgtgc ggccaccccc tgcaacctga
     1441 tgcctgccct ggagcaaagg agaagacccc atgtgcccga ctctacgcgt acagatcgtt
     1501 gacagcaccc tccgcgaggg cgagcagttc gccggggcgc acttcaccag cgccgcccgc
     1561 gtcgccatcg ccgaggccct ggatgccttc ggcgtcgagt acatcgaact gacctcgccg
     1621 gtcgcttcgc cgcaatccgc ccaggatctg cggacgatcg cggccctccc cctgcgggcg
     1681 cgggtgctga ctcacacccg ctgccgcctg cccgatgtcc agcttgccgc cgactgcggc
     1741 gtggacgggg tcaatctcct gttcggcacc tccccctatt tgcgcgccta cagccacggg
     1801 cgcagcctcg acgccattct cgccgagacc accacggtca tccgcttcct gcaagagcgg
     1861 ggcatcgaag tccgcttctc ctgcgaagac gccttccgca caccgctcgc cgacctgctg
     1921 cgcgtctacc gggcggtcga tgccctgggc gtgcagcgcg tcggcatcgc cgacacggtg
     1981 ggcatcgcca caccgcggca ggtgacggcg gtggtggcgg cggtgcgcgc agcggtgggc
     2041 tgcgacatcg agttccacgg ccacaacgac ggcggctgcg ccatcgccaa cgccgacgcc
     2101 gccctgcacg gcggggccac ccacatcgac accacggtgc tgggcatcgg cgagcgcaac
     2161 ggcatcgccg cgctgagcgg cctcatcgcg cgcctctacc tgaccgaccc ggccctggtc
     2221 gcccactacc acctgccgct gctgcccgat ctggacgcca tggtcgcccg catggtgggc
     2281 gtggagatcc cgttcaacgc ctgcatcacc ggggcgacgg cgttcacgca caaggccggc
     2341 atgcacaccc acgccgtgct gcaagagccg cgcgcctacg aggcgctgga cccggcagcc
     2401 tttggccgcg agcgcgccgt gctgatgggc caccgcctga ccgggcgcca cgccctggcc
     2461 caccgcgccg ccggcctcgg tctgcaactg agcgacgacg ccctgcgcgc gacgactgat
     2521 caggtgaaag ccctggccga cgccggcccg ctgacccagg tgcaggtcga tgcgctgctc
     2581 cacgagcacg ccgcgcccga cgggatggcc cccaatgctt gatcgtaacg cccaaccacg
     2641 aaaggaacga ccgatggctc agactcttgc cgaacagatt ctctcccacg ccgccggacg
     2701 gcccgtccgg gcgggcgagg tgcataccgt cgccgtggac ctggtgatgg tccacgacag
     2761 catcgctccc agcgtgatcc gcgtcttgca ggacgagcta ggctgcgcgc gggtgttcga
     2821 cgccgaccgg gtcgccgtgg tgatcgacca cgtctcgccc gcggccaatg tgcagaccgc
     2881 cgaggcgcag cgccgcctgc gcgcctgggt gcgcgagcag ggcatcgccc actggttcga
     2941 gtcggggcgc ggcatcgccc accaggtgct gatcgaggag cggctggccc gccccggtcg
     3001 cctgatcacc ggctcggact cgcactccac cgcctacggc gcggtggccg cgttcggcac
     3061 cggcatgggc gcgaccgaca tcgccctggc cctcgccacc ggccacacct ggctacgcgt
     3121 gccggagacc atcctggtcc gcgcccgtgg caatttcggg tcgggcgtgg ggccgaagga
     3181 cctggccctc tggctggcgc gcaccctcgg cgcggacggc gcgacttacc gcgccgtcga
     3241 ataccacggc ctcgacttcc tgaccgtggg cgaccgcacg acgctgacca ctatggcgct
     3301 ggaggtcggg gccaaggcgg gtatcgtcgc cccggcgggc ctgccggatc gctatccggt
     3361 gcccgactgg ctgcgcgtcg aggacggcgc ccgctacgag cggatcatcg aggtcgatct
     3421 ggacgcgctc agcccgcaga tcgccgtgcc gaacaccgtg gacaacgtgg tcgatctgcg
     3481 cgacctgggc cgggtggcgg tggatgtggt ttacctcggc acctgcacta acggccgcca
     3541 cgccgatatg gctgccgccg cgcgcatctt gcaagggcgg cacgtcgcgc ccggcgtgcg
     3601 catgatggtc gtcccggcgt cgagcgaagc gctgcaggag gccattgccg acggcacgct
     3661 cacgacgctg ctgagcgcgg gcgcgaccat cggcacgccc ggctgcgggg cgtgcatcgg
     3721 gcgccacatg ggcgtgctgg cgcccggcga gatctgcctg ttcaccggca accgcaattt
     3781 ccgcgggcgc atgggcgcgc cggaggcggg tatctacctc gcctcgcccg aagtcgccgc
     3841 cgccaccgcc gtcgccgggt acatcgccga cccgcgcgag ttggacgcgc tacccgcctg
     3901 accattcgct aacattatgt aaaggagcaa agctatgtcc cgcgtatggc gcttcggcga
     3961 cgatgtgaac accgaccaga tcgtgcccgg ccgttatgcc ccctacatga catccgaggc
     4021 cgagctacgc aaatacccgt ttatcgaggc ccggcccgag ttcgccgcga ctgtgcagcc
     4081 gggcgatctg atcgtcgccg gacgcaactt cggctgcggc tcgtcgcggg aacacgcgcc
     4141 ctgggcgctg atggacttcg ggatccgctg cgtggtctcc accagcttcg ccgacatctt
     4201 ctacaacaac tgcttccaga acggcctcct gcccgtggtg ctgaaggcgg acgaggtgca
     4261 gcagctcatg gacgaggcgc gcggcggcaa ccacgtggtc agcgtcgacc tggaggcgca
     4321 gaccgtgacc tcgccgtcgg gcgcggtgtt cggcttcgac atcgatccga agcgcaaggc
     4381 gaagatgctt ggcggcctcg acgcgatcgg cgagacgctg acccgcgccg gcgacatcga
     4441 cgtcttcgag atgaagcgcg cgctcagcca accgtggctg gaagaggcat gaccctcctc
     4501 atcgccggca cggtgcgcgt gccgcccgag aacgtggagc gcttcaagcc gcacatggag
     4561 gc
//
LOCUS       DASUYC010000975         2331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_857035,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000975 DASUYC010000000
VERSION     DASUYC010000975.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857035"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VF276_15980"
     CDS             complement(<1..552)
                     /locus_tag="VF276_15980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608400.1"
                     /translation="MNRFEPDTWLAALLRPVAMAAPDSYVYVEMMAPDFRWLFVLLLL
                     ALLLVMRRRSRAGSAGAVAATGADSRPAAVFVLVAALVVAFVPWLMTSGNGRYFLVGL
                     LIVGPVCVGLAWRLPTTRALRLTLAAGMVALQAFAVQQSAPWQAWGLAPWKEAPYFQV
                     DLPPEAKTEPATYVTMSVISYSLL"
     gene            complement(559..1350)
                     /locus_tag="VF276_15985"
     CDS             complement(559..1350)
                     /locus_tag="VF276_15985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608401.1"
                     /translation="MLRWEGLLVCLLTVAFFASVPLAQGELGLGSDALNHHIYLGWTA
                     QQHRFDLDFVAAGYQSYTFPYLYWPLYQMAAAGWSGVTVGVTLAALQAVLVAPPVWLL
                     ARACMPGATWFDLAMRALGVALAFLTGVALSVLDATMNDLLAAAPLLWAVALAVQAMP
                     GAGSHARTVRWLVLFSGLCAGAAVAAKLSNGPLAILMPGLWWLCGRGWRPRVAAVALG
                     SAATLLGFAALYGYWGWLLWRHFGNPIFPFHHHWFEPLRRWAGMG"
     gene            complement(1389..>2331)
                     /locus_tag="VF276_15990"
     CDS             complement(1389..>2331)
                     /locus_tag="VF276_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002787625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX6608402.1"
                     /translation="GIAAARGRFVVMGDADGSYDFTSLLAFLERLRGGAQLVMGNRFQ
                     GGIAPGAMPWLHRYLGNPVLSFIGRLFFRVPIGDFHCGLRGFSREAILGLGLVSPGME
                     FASEMVAKAALAGLSIEEVPTTLRPDGRDRPPHLRTWRDGWRHLRFLLLFCPRWLFLY
                     PGLLLMLLGLAGFAFGAVGRVLGVHSLLYTAAATVLGGQLIQLVLLTKWIGIVSGVVP
                     PQRWLARLSPFLKIETGLLAGLLFMLLGLAVSVGLTVDWGAAGFGALEPEQTMRVAIP
                     AVTLMILGAQVAAGSLFAGALNFNWNSAERQGRAGQS"
BASE COUNT          488 a          851 c          743 g          249 t
ORIGIN      
        1 cagcaacgaa tagctgatga ccgacatggt gacgtaagtg gcgggttccg ttttcgcctc
       61 gggcggcaaa tccacctgga aatacggcgc ctccttccac ggggccagcc cccaggcctg
      121 ccacggcgcg ctctgctgca cggcaaacgc ctgcaaggcc accatgcccg cggccagcgt
      181 gaggcgcagc gcgcgggtcg tcggcaagcg ccaagccagc ccgacgcaca caggtcccac
      241 gatgagcaac ccgaccagga aatagcggcc gtttcccgat gtcatcagcc aaggcacgaa
      301 agccacgacc aatgccgcca ccaagacgaa gaccgcggca ggccggctgt cagcgccagt
      361 tgcagcgaca gcgcccgcac tgcctgcgcg tgagcgccgg cgcatgacca acagcagcgc
      421 cagcaagagc agcacgaata gccagcggaa atccggcgcc atcatttcga catacacata
      481 gctgtccggc gcggccatcg caacgggccg cagcagcgcg gccagccagg tgtcgggttc
      541 gaagcggttc atggcgcgtc agcccattcc cgcccaacgc cgcaagggct cgaaccaatg
      601 gtgatgaaag ggaaaaatcg ggttgccgaa gtggcgccac aacaaccagc cccagtagcc
      661 atagagcgcc gcaaagccaa gcaaggtggc cgcgctgccc agggcgactg ccgccacccg
      721 cggcctccag ccgcggccgc acagccacca caaccccggc atcaggatgg caagtgggcc
      781 gttactgagc ttggccgcca cggcggcccc ggcgcacagg cccgagaaca gcaccaacca
      841 gcgcacggtc ctggcatgac tgcccgcgcc cggcatggcc tgcaccgcca gcgccaccgc
      901 ccataacagc ggcgccgcgg ccagcagatc gttcatcgtc gcgtccagca ccgacagggc
      961 cacgcccgtc aggaaagcga gcgccacgcc cagcgcgcgc atcgccaggt cgaaccaggt
     1021 cgcgccgggc atgcaggcgc gcgccaggag ccacacgggc ggcgcgacca gcacagcttg
     1081 cagcgccgcc agcgtcacgc cgacagtcac gccggaccag cccgcggccg ccatctggta
     1141 cagcggccaa tacagatagg ggaaggtata ggactggtaa cccgccgcga cgaaatcgag
     1201 gtcgaaccga tgctgctgcg cggtccagcc caggtagata tggtggttca gcgcgtccga
     1261 acccaggcca agttcaccct gcgccagcgg cacggacgcg aagaaggcca cggtcagcag
     1321 gcaaaccagc agcccttccc agcgcagcaa ggctgcgagc gccgaaggcc gtgcggctgg
     1381 ccgctggttc acgactggcc cgccctgccc tggcgctccg ccgaattcca attgaagttc
     1441 agtgccccgg cgaacagcga gccggccgcc acttgcgcgc ccaggatcat cagcgtcacg
     1501 gcgggaatgg cgacccgcat ggtctgctcc ggctccagcg ctccgaaacc cgcggcaccc
     1561 cagtcgacgg tcagtccgac ggacaccgcc aatcccagca gcatgaacag cagccccgcg
     1621 agcaagccgg tttcgatctt gaggaagggt gacaagcggg ccagccagcg ctgcggcggc
     1681 accacgcccg atacgatgcc gatccacttg gtcagcagca ccaattgaat cagctgcccg
     1741 cccagcacgg tggcggccgc cgtgtacagc agggagtgca caccgagcac ccggcccacc
     1801 gcgccgaagg caaaccccgc cagacccagg agcatgagca gcaaaccggg gtacaggaac
     1861 aaccagcgag ggcaaaacag cagcaggaag cgcagatgcc gccagccgtc gcgccaggtg
     1921 cgcaggtgcg gcgggcggtc gcgtccatcg ggccgcaggg tggtgggcac ttcttcgatc
     1981 gacaggcccg ccagcgccgc cttggccacc atttcgctgg cgaactccat gccgggcgaa
     2041 accaggccca ggcccagaat cgcctcgcgg gaaaagccgc gcagcccgca atgaaaatcg
     2101 ccaatgggca cgcggaagaa caggcggccg atgaaactca gcaccgggtt gcccaggtag
     2161 cggtggagcc acggcatggc gcccggcgca atgccgccct ggaagcggtt gcccatcacc
     2221 agttgcgcgc ccccgcgcag ccgctcaagg aaagccagca ggctggtgaa gtcgtagctg
     2281 ccgtcggcgt cgcccatgac gacgaagcgc ccgcgcgccg ccgcgatgcc c
//
LOCUS       DASUYC010000976         3176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_412776,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000976 DASUYC010000000
VERSION     DASUYC010000976.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412776"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VF276_15995"
     CDS             complement(<1..211)
                     /locus_tag="VF276_15995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608403.1"
                     /translation="MKRMLKWFLPALIVVEIALVWGNVLDAGTALLVGGAVEGLLLLV
                     VAGEIALMVRDYQRNRAAGLNVEAAV"
     gene            complement(232..996)
                     /locus_tag="VF276_16000"
     CDS             complement(232..996)
                     /locus_tag="VF276_16000"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX6608404.1"
                     /translation="MVAKELIDARWKLIIGGALTVLVVAGLSVLYTVVRDLANNPSIA
                     GMPEAIKNDLQLSVANFGLYAWGQWFPKNGPLLLGGLAAFLGAGLIAGEVGKGTIYFL
                     LSKPVSRERVLITKYLVNAAILLAVTLLGSIVMLLTGVILGQSVPVDRILLATVLLWL
                     GTMSILGVALLFSTLFDDMLRPAGLAILVAIVIGLPGVVGQFVPGWADWSLPSYWASL
                     SGFLGTAFPAKEFAICLVTAILPVAGALAVFRKRAF"
     gene            complement(986..1948)
                     /locus_tag="VF276_16005"
     CDS             complement(986..1948)
                     /locus_tag="VF276_16005"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6608405.1"
                     /translation="MTTMATAATGAQHTSAPASAIEIQDLVVSYGNKRAVDGLNLQVP
                     RGAVYGFLGPNGAGKTTTIKALLGFRPANGGSARVLGYDIGTQSLEVRAHVGYVSEVN
                     SMYDYLTIPQTCDFCRQTSRRWNQAVVDRYITLFGLPPRARVGQLSKGMKSQLALSLA
                     LGSEPDLLILDEPTAGLDPVARHEFLNTLIRDIAAEGKTVFFSSHILSEVEAVADRVG
                     IIRDGKLLVSDDLDHLKQTQRVLKLTYAEVPPPDEIAALRALPGVARVEQEGRGVRVI
                     ARGDVDALAAQLQTRPYPVRDLDSVNLNLEDLFLEYMKEGTDGR"
     gene            complement(1945..2349)
                     /locus_tag="VF276_16010"
     CDS             complement(1945..2349)
                     /locus_tag="VF276_16010"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX6608406.1"
                     /translation="MPIWITIERHSGVPIYMQIAEQFRHAMEVGALQPGEQLPTVRQL
                     AGDLAIAPNTIVQAYEKLQGMGLIESRPGAGTVVLAQANGALRHQQIEALYERLQRLV
                     HDAAGLGIAPADLEGRFQAEVARFYKESEGRQ"
     gene            complement(2557..2790)
                     /locus_tag="VF276_16015"
     CDS             complement(2557..2790)
                     /locus_tag="VF276_16015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608407.1"
                     /translation="MNNDTCPHSRVVISRKHESWVRHTIYEGRLQGRRVEVGDTNGTI
                     RVRCFDCGYERRFTRSDEKPAWIEELLPQLDNT"
     gene            complement(2809..>3176)
                     /locus_tag="VF276_16020"
     CDS             complement(2809..>3176)
                     /locus_tag="VF276_16020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608408.1"
                     /translation="IAGGVALRYLAQEAAKLVPVGGSAVAAGVAAAGTWAMGQVAIQY
                     FESGKRLSRGELREAYQRLLSRPEPHFEGEGEGEEALPVGPAVAVPPVPPAPPRPGAP
                     AGADSGRRLRLPFGRDRQD"
BASE COUNT          498 a         1088 c         1079 g          511 t
ORIGIN      
        1 tgacggcggc ttcgacgttc aggcccgccg cccggttgcg ctggtagtcg cgcaccatca
       61 gcgcgatctc gcccgcaacg acgagcagca gcaggccctc gacggccccg cccacgagca
      121 gcgcggtgcc cgcgtcgagc acattgcccc agaccagggc gatctccacc acgatcaggg
      181 cgggcaggaa ccatttcagc atccgtttca tggccgcgct gctcctttac gctaaaatgc
      241 ccgtttgcgg aacaccgcca gcgcgccggc caccgggagg atggccgtca ccaggcagat
      301 ggcgaactcc ttggccggga aagccgtgcc caggaagccc gacaggctgg cccagtagct
      361 cggcaggctc cagtcggccc agccgggcac gaactggccg accacgccgg gcaggccgat
      421 cacgatggcg accaggatcg ccagccccgc cgggcgcagc atgtcgtcga acagggtcga
      481 gaaaagcagc gccacgccaa ggattgacat cgtgccgagc cagagcagca ccgtggcgag
      541 caggatgcgg tcgaccggca cgctctggcc caggatcacc cccgtcagca gcatcacgat
      601 gctaccgagc agggtgacgg ccagcaggat ggccgcgttc accaggtact ttgtgatcag
      661 cacgcgttcg cggctcaccg gcttgctcag caggaagtag atcgtgccct tgcccacctc
      721 cccggcgatg aggcctgcgc cgaggaaggc ggccagcccg cccagcagca gcggcccgtt
      781 cttggggaac cactggcccc aggcgtacag accaaagttg gcgacggaca actggagatc
      841 gttcttgatg gcctccggca tcccggcaat gctcgggttg tttgccagat cgcgaacgac
      901 ggtatacagg accgacaggc ccgccaccac cagtaccgtg agcgctccgc cgataatcag
      961 cttccagcgg gcgtcgatta gctccttagc gaccatcggt gccctccttc atgtattcga
     1021 ggaagaggtc ttccaggttc agattgacgc tgtccaggtc gcgcaccggg taggggcggg
     1081 tttgcaactg cgccgccagg gcgtccacgt caccgcgggc gatcacccgc acgccgcgcc
     1141 cctcctgctc gacccgcgcc acgccgggca gtgcccgcag cgcggcgatt tcgtcgggcg
     1201 gcggcacctc ggcgtaggtc agcttcagca cgcgctgggt ctgcttcagg tggtccaggt
     1261 cgtcgctcac cagcagcttg ccgtcgcgga tgatgcccac ccggtcggcc accgcctcca
     1321 cctcagagag gatgtgcgac gagaagaaga cggtcttgcc ctcggcggcg atgtcgcgga
     1381 tcagggtgtt gaggaactcg tggcgcgcga ccgggtcgag gcccgccgtc ggttcatcga
     1441 ggatgagcag gtcgggttcg ctgcccaggg ccaggctcag ggccagttgc gacttcatgc
     1501 ctttggaaag ctgccccacc cgcgcccgcg gcggcagccc gaacagcgtg atgtagcggt
     1561 cgaccacggc ctggttccag cggcggctgg tctggcggca gaagtcgcag gtctgcggga
     1621 tggtcaggta gtcatacatg ctgttgacct cgctgacgta gcccacatgg gcgcgcactt
     1681 cgaggctctg cgtgccgatg tcgtagccca gcacgcgggc gctaccgccg ttggcgggac
     1741 ggaagccgag cagcgccttg atcgtcgtcg tcttgcccgc gccattgggg ccgaggaagc
     1801 cgtagaccgc gccccgcggc acctggagat tgaggccgtc caccgcgcgc ttgttgccgt
     1861 agctgacgac cagatcctgg atttcgatgg ctgatgcggg cgcggatgtg tgctgcgcgc
     1921 ccgtcgccgc cgtcgccata gttgtcattg ccgtccctcg ctctctttat aaaaccgggc
     1981 tacctcggcc tggaacctac cctccaggtc cgccggtgcg atgcccagcc cggcggcatc
     2041 gtgcaccagg cgttgcaggc gctcatacag cgcctcgatc tgttgatggc gtagcgcgcc
     2101 gttggcctgg gcgagcacca cggtgccggc gccgggccgg ctctcgatca ggcccatgcc
     2161 ctgcaacttc tcgtaggcct gcacgatcgt gttcggcgcg atggcgaggt cgcccgccag
     2221 ttggcgcacc gtgggcagtt gctcgcccgg ttgcagcgcg ccgacttcca tggcatggcg
     2281 gaactgctcg gcgatctgca tatagatcgg caccccgcta tggcgctcga tggtgatcca
     2341 gatgggcata ggctcccgtc caacttgtcg catattacta acacaatgat acacttagac
     2401 ggcgtgggag tcaataggtc gcaagacgga gggccaaacg ggcggatgtc gcgagggttg
     2461 ggggatgcgg gcgaaccggt gcgccgccag ggggcggggg tccggtcgcg gaaagtggga
     2521 cggagccgcg ctccgccgcg gccccgcttt tcgtgtctac gtgttgtcta actgcggcaa
     2581 tagctcctcg atccaggccg gtttttcgtc gctgcgggtg aagcggcgct cgtagccgca
     2641 gtcgaagcag cgcacgcgaa tggtaccgtt ggtgtcgccg acttcgaccc ggcggccctg
     2701 gaggcgcccc tcgtagatcg tgtgccgcac ccaggactcg tgcttgcgcg agatgaccac
     2761 gcgcgagtgc gggcacgtgt cgttgttcat aggctgccgg cctcctgctc agtcctggcg
     2821 gtcgcgcccg aagggcaagc gcagccggcg cccgctgtcg gcgcccgcgg gcgcgccggg
     2881 ccggggcggg gcgggcggca cgggcggtac cgccaccgcg ggtcccaccg ggagggcctc
     2941 ttcgccttcg ccctcgcctt caaagtgcgg ctcggggcgg ctgagcaggc gctggtaggc
     3001 ttcgcgcaac tcgccccggc tcaggcgctt gccgctctcg aagtactgga tggcaacctg
     3061 gcccatggcc caggtgcccg cggcggccac tcccgcggcc accgccgacc cacccaccgg
     3121 cacaagcttg gcggcttcct gggccaggta gcgcagagcc accccgccgg cgatgg
//
LOCUS       DASUYC010000977         3163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_313483,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000977 DASUYC010000000
VERSION     DASUYC010000977.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313483"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(309..2729)
                     /locus_tag="VF276_16025"
     CDS             complement(309..2729)
                     /locus_tag="VF276_16025"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6608409.1"
                     /translation="MEHVATFGGWLSAYRQAVHLQRAELAARIGCAVVTLRKIEADER
                     RPSRDMAERLAEVLAIPEQRRDVFIRVARGELPLARLAIAPPAAPGPTNLPAPTTALA
                     GREREIADIQAILARPEVRLLTLTGAPGVGKTRLALAAAQEMSDRFTDGIFFVALAPL
                     SDPKLVLVSIAHALHVGTSGGQTLDERLGRYLRTRRVLLVLDNFEHLLAAAPHLTELL
                     RAAPQLKLLVTSRGALELSGEHRFTVLPLAVPPAAEHLRQPLTVAEAEARYPAVALFM
                     QRAQAVHPGLALTSADVAAVGEICRRLDGLPLAIVLAAARTTLFTPRELLARLDDRFA
                     LLSAGARDLPARHMTLEAAIDWSYGLLAPADQQLFRRLSVFAGGCTLAAAQEVCNAGG
                     AVGRDVVGGITALVEGSLLQREAGADGQSRFEMLETIREYAASQLSASGEADSLRGQH
                     AAYYLRLAEAAEQAWDGPAEWDWLGRLVVERDNLRAALRWAIEAQDAALALRLNAGLF
                     SFWSLCSALPEARRWVEAALAVPRPPNPALDPVEAKVLHVAGYMAAAMADYAQALDYF
                     EQGLARYRALGDFRGIAWSIRSCAFAHMLRDEYAQADQMYTESLRLCEAHGDAWGHAW
                     SLYALAFLQLAQGDLVAARPALENALVLLRRENMAFAAFRALLALGDALFEQGDLPGA
                     EARYREALLLSRETPLLTFITTGLEGLGMIASARQQPLRAVRLWGAAAALREVTDERR
                     WPVFQRIYDRAVQAAREQVPATEWDAAWAAGRALPAAQAVAEALESAEPLPRLEEESL
                     LIGRNLVY"
BASE COUNT          471 a         1110 c         1135 g          447 t
ORIGIN      
        1 cttgcgcgtc cctaccaaaa cgcgtactcg actcatccca gactcctttc agtactgcca
       61 acgcggcgcg cgcggcgcct gtgccatacg tactccagtc aaacggaccg gcgtgggatc
      121 ggttgctcgc cgccatccct ccggctgctt gctttcccgg tatatcccgg ccagggcggc
      181 aaaaccggaa gtgtcccttc gccgggtggc cgcccggtgg gcgggcagtg ctggatgggc
      241 cgcaggcgcg gcgcggggcc gcgaaacctc cgtggacggg tggcggtgcc cggtgggccg
      301 ggtgcctgtc aataaaccag gttgcgcccg atcagcagcg actcttcctc caggcgcggc
      361 agcggctcgg cggactccag ggcctcggcc acggcctgcg ccgcgggcag ggcacgccct
      421 gctgcccagg cggcgtccca ctccgtggcc ggcacctgtt cgcgggcggc ctggaccgcg
      481 cgatcataga tgcgctggaa gaccggccag cgccgttcgt ccgtcacttc gcgcagggcc
      541 gcggcggcgc cccacaaccg caccgcgcgc aacggctgct gccgcgcgct cgcgatcatg
      601 cccagcccct ccaggccggt ggtgataaag gtcagcagcg gcgtctcgcg gctgagcagc
      661 agtgcctcgc ggtagcgcgc ctcggcaccc ggcaagtcgc cctgctcgaa cagcgcgtcg
      721 cccagcgcga gcagcgcgcg gaaggcggca aacgccatgt tctcgcggcg cagcagcacc
      781 agagcattct ccagcgcggg ccgggcggcc accagatcgc cctgcgcgag ttgcaagaaa
      841 gccagggcgt acagggacca cgcgtggccc caggcatcgc cgtgggcttc gcagagccgc
      901 aggctttcgg tgtacatctg gtccgcctgt gcgtactcat cgcgcagcat gtgggcgaaa
      961 gcgcaactcc gtatcgacca ggcgatcccg cggaagtcgc ctagcgcccg gtagcgtgcg
     1021 agcccttgct cgaagtagtc gagcgcctgc gcgtagtcgg ccatggccgc cgccatgtag
     1081 cccgcgacgt gcaacacttt ggcctccacc ggatcgagcg cggggttcgg gggtcggggc
     1141 acggccaggg ctgcttccac ccagcggcgc gcttcgggca gggccgagca gaggctccag
     1201 aacgagaaca ggccggcgtt gagccgcagc gccagcgcgg catcttgcgc ctcgattgcc
     1261 cagcgcagcg ccgcgcgcag attgtcccgc tcaaccacca gccggcccag ccagtcccac
     1321 tccgccgggc cgtcccaggc ttgctcggcg gcctccgcca gccgcaggta ataggccgcg
     1381 tgttgcccgc gcaggctgtc cgcttccccg ctggcgctga gttggctcgc ggcatattcg
     1441 cggatcgtct ccagcatctc gaagcgcgac tgcccgtcgg cgcccgcctc gcgttgcagc
     1501 aggctgcctt cgaccagggc cgtgatcccg ccgaccacgt cgcgccccac cgcgccgccg
     1561 gcgttgcaga cctcctgggc ggcggccagc gtgcagccgc cggcaaacac gctcaggcgg
     1621 cgaaacaact gctggtcggc gggcgccagc aggccgtaac tccagtcgat ggcggcttcg
     1681 agggtcatgt gccgggcggg caggtcgcgg gcgccggcgc tcaacagcgc gaagcggtcg
     1741 tcgaggcgcg ccagtagttc gcgcggagtg aagagcgtgg tgcgcgcggc ggccagcacg
     1801 atcgccagcg gcagcccgtc gaggcgccgg cagatctcgc ccaccgctgc gacatcggcg
     1861 gaggtcagcg ccaggccggg atgcaccgcc tgggcgcgct gcatgaacag ggcgacggcg
     1921 ggatagcgcg cctcggcctc cgccacggtg agcggctgcc gcaggtgctc cgcggcgggc
     1981 ggcacggcca gcggcagcac ggtgaagcgg tgctcgcccg acagttcgag cgcgccccgg
     2041 ctggtcacca gcagcttaag ctggggggcg gcccgcagca gttcggtcag gtggggcgcg
     2101 gcagccagga gatgctcgaa attgtcgagg acgagcaaga cccgccgcgt gcgcaggtag
     2161 cgccccaggc gttcgtcgag ggtctggccg ccggatgtac ccacgtgcag ggcgtgcgcg
     2221 atggagacga ggacgagctt ggggtcgctc agcggcgcga gggcgacaaa aaagatgccg
     2281 tcggtgaagc ggtcgctcat ctcctgggcc gccgccagcg ccaggcgtgt cttgccgacg
     2341 cccggcgccc cggtgagcgt cagcaggcgc acctccggac gggccaggat ggcctggatg
     2401 tcggcgattt cgcgctcgcg cccggccagc gcggtggtgg gggccggcaa attggtgggg
     2461 ccgggggcgg caggcggggc gatcgccagg cgcgcgagcg gcagttcgcc gcgggccacg
     2521 cggataaaca catcgcggcg ttgctccggg atcgccagca cctcggccag gcgctcggcc
     2581 atgtcgcgcg agggccggcg ctcgtccgcc tcgatcttgc gcagcgtgac caccgcgcag
     2641 ccgatgcggg ccgccagttc ggcgcgttgc aggtggacgg cctggcggta cgcgctgagc
     2701 cacccgccga acgttgccac atgctccatc acacatcctc cttcgcccca tcgcgaccgg
     2761 gctggcggtc gggcgatatg gcggggttgg atcctcgcgc tgccgccgcc attgtatcac
     2821 tttttgtatc cgggcgggta cccgcgtgga taccacaggc cccggcggcg gggcgataat
     2881 ataaccaagc acgatgcggc aagcccgcat cgggaatcgg tcggatcgac ggggtggtcg
     2941 cggtaggacg cgcctggccc ggtcaagcac ataaggagaa ggtaggatgg ctactaatat
     3001 acagacggcg caaggcaccc ccaacgcgcc taacacggcc atggcgaagc gggccgcgct
     3061 gagcgcgttc ctgggcagca cggtcgagta cttcgatttc gtgctcttcg ccacggcatc
     3121 ggcactcgtc tttaacaagg ttttctttca ggcgttggga tcc
//
LOCUS       DASUYC010000978         3538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_625020,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000978 DASUYC010000000
VERSION     DASUYC010000978.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625020"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="VF276_16030"
     CDS             complement(<1..316)
                     /locus_tag="VF276_16030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608410.1"
                     /translation="MEHNSVIPATPATASADAAPPSGPAWMQRARGLLGNKWLRRVLQ
                     IAVIGVVFFFLARAVWMEWPDIQAYPWHLEIGYVLAALLVLLVRGPIICLGWRMILRF
                     MGY"
     gene            complement(430..2091)
                     /locus_tag="VF276_16035"
     CDS             complement(430..2091)
                     /locus_tag="VF276_16035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HEX6608411.1"
                     /translation="MSEQPTFIVSIEITILWMLVAVAAVALVTKRIRLPYTVALVIAG
                     LIIAFVPGTPKVDLTPDLILSVFLPTLIFEAAYNLDFQHLRDNIRTISVLAIPGVLLT
                     AGVVAVLMHYAAGFDWVVALLFGAIVAATDPVSVVATFKQLGAPSRLRTIIEGESLFN
                     DGTSLVLFRVLVGILAAGALDPASSVAQFFLVIAGGLLVGLATGYIVARLMTQFDDYL
                     VETVMTVILAYGTYFLAEQLHVSGVIAVVVAGLVVGNYAQTTSFSPTSRIAVGLSWEF
                     FGFLTNSLIFLFVGLQVHLARLFDLWHVVVAIAAVVLSRAVAVVMVSWVLRILRIDRP
                     LAWNWQVLLVWGGLRGSLSLAMALSLPYVVGPASEPFRERQDILVMTFGVIIFTLLVQ
                     GLTLRPLLERLGLVGTDQQRAEYERTRAALRVVRAAQNTLDREVRGGIVTPLIAADLR
                     AEYEAREAALLARLESFQISDQDLREQQLRSARRHLLTVERSTIRTLLTEGAISDESG
                     RELVTEIDARLAGLEAAAEPDAAAAAPPAPAPNAPRIREEAPPPG"
     gene            complement(2312..>3538)
                     /locus_tag="VF276_16040"
     CDS             complement(2312..>3538)
                     /locus_tag="VF276_16040"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEX6608412.1"
                     /translation="FRQDFFLAPNEATPGYRRGPLPAGEWHIMLGPEEVTAGGCAYTV
                     TVTCTVAARAEDVPAPVPYPRYEPGVLERGARWYRGNLHAHTYHSDGDNSVAEMAAEH
                     RRWGLDFGAMTDHNLVNPELALGAHPDFLWLPGEEVTTPWGHLNVWGLDADDWIDFRC
                     RDLTGMEQIIAAARARGALTSINHPKEDGPDWRFPAFPETDAFEAWQAPWFLSNYVTL
                     SLWEGLLDSGHYPTAVGGSDLHRISTPDHPYPYLIGNPTTWVYAEELSIPAILAGIRA
                     GHVFISADPTGPQLLLEAHTPHGRALPGDRVLLHEGEPASVRVEVLGGEGRMLRLLGE
                     HGVLHESLILSERQVVEYQVRDTFVLPGYIRAELIAPLDPEVDLATEPGALWVDAMTN
                     PVYFRLASLEPRTEEA"
BASE COUNT          647 a         1241 c         1144 g          506 t
ORIGIN      
        1 ggtagcccat gaagcgcagg atcatgcgcc agcccaggca gatgatcggc ccgcgcacca
       61 gcaagaccag cagggccgcc agcacgtagc caatctccag gtgccagggg tacgcctgga
      121 tgtcgggcca ctccatccag accgcgcgcg ccaggaagaa gaagactacg ccgatcaccg
      181 cgatctggag cacccggcgc agccacttgt tgcccagcag cccgcgcgcc cgctgcatcc
      241 aggccgggcc cgacggcggg gcagcgtccg cggacgccgt cgccggggtg gccggtatca
      301 cgctattgtg ctccatcggg tctccgatcc acgctgccag gctccacgaa ataagcgttg
      361 caaaataagg atgcgccgca ggccacttct gcaggcgccc tggtatgcta cccaatcccc
      421 ggctatttgt caacccggcg ggggcgcttc ctcgcgtatc cgcggggcgt tgggcgcggg
      481 ggcgggcggc gctgcggcgg cggcatcggg ctcggcggct gcttccagcc cggccagccg
      541 ggcgtcgatc tccgtcacca gttcgcgccc cgactcgtcg ctgatcgcgc cttcggtcag
      601 cagggtgcgg atggtgctgc gctccaccgt gagcaaatgc cgccgtgccg agcgcaactg
      661 ctgctcgcgc aggtcctggt cgctgatctg gaaactctcc aggcgcgcca gcagcgccgc
      721 ttcgcgcgct tcatactcgg cgcgcaggtc cgcggcgatc agcggcgtga cgatgccgcc
      781 gcgcacttcg cggtcgagcg tgttttgcgc tgcgcgcacc acgcgcaagg cggcgcgcgt
      841 gcgctcatat tctgcccgtt gctggtcggt gcctaccagg ccgagccgtt cgagcagcgg
      901 gcgcaacgtc agcccctgca ccagcagcgt gaagatgatg accccgaagg tcatcaccag
      961 gatatcctgg cgctcgcgga acggttcgct cgccggaccg acgacatacg gcagcgacag
     1021 agccatcgcc agcgatagcg acccgcgcaa gccgccccac accagcagca cctgccagtt
     1081 ccaggccagc ggccggtcga tgcgcaagat ccgcagcacc cagctcacca tcaccacggc
     1141 caccgcccgc gacaacacca cggcggcgat cgccacgacg acatgccaga ggtcgaacag
     1201 gcgcgccagg tggacttgca ggccgacaaa caggaagatc agcgagttcg tcaagaagcc
     1261 gaaaaactcc caggacaggc ccaccgcgat gcgcgaggtg ggggagaagc tcgtggtttg
     1321 cgcgtagttg cccaccacca gccccgccac gaccaccgcg atcaccccgg acacatgcaa
     1381 ctgctcggcc aggaagtacg tcccgtaggc caggatcacc gtcatcaccg tctcgaccag
     1441 gtagtcgtcg aactgcgtca tcaggcgggc gacaatatag cccgtcgcca ggccgaccag
     1501 caagcccccg gcgatcacca ggaagaactg ggcgacactg ctcgccgggt ccagcgcgcc
     1561 cgcggccaga atcccgacca gcacgcggaa gagcaccagc gaggtgccgt cattaaacag
     1621 gctctcgcct tcgatgatcg tccgcaagcg cgagggcgcc cccagttgct tgaacgtggc
     1681 cacgaccgac accgggtccg tggccgcgac gatggccccg aacaacaacg cgaccaccca
     1741 atcaaagccg gcggcatagt gcatgaggac ggcgaccacc cccgccgtga gcagcacacc
     1801 cggaatcgcc agcacgctga tcgtgcgtat attgtcgcgc aggtgctgga agtcgaggtt
     1861 ataggccgcc tcgaagatca gcgtgggcaa aaacaccgac aggatcaggt cgggcgtcag
     1921 gtccacttta ggagtgccgg gcacgaaggc gataatgagg ccggcgatga ccagggcaac
     1981 ggtatagggc aggcgaatgc gcttcgtgac cagggcaacc gccgccaccg ccaccagcat
     2041 ccacaggatg gtgatctcaa tgctgacaat aaacgtcggt tgctcgctca ccgcacccgc
     2101 tcctcatcgc cggcggcaac gtaccgcgct aagacaggac gaactccacc cggcgggcgg
     2161 agttcgcata atcgctttag cagcaaacta tatgccctgg taccgaaggc ccgcgtcgta
     2221 cggcaagcaa gcgacctatc cgcctaccgg cccagccttc tctgtagggg ccggtttcaa
     2281 accggcccgc gccgccggat cctggcgacg gctacgcctc ctccgtccgc ggctccagcg
     2341 aggccaggcg gaaatagacc ggattggtca tggcgtccac ccagagcgcg cccggttcgg
     2401 tcgccagatc cacctccggg tcgagcgggg cgatcagttc ggcgcgaata tagcccggca
     2461 atacgaaagt atcgcgcacc tggtactcga ccacctgccg ctcactcagg atcaggctct
     2521 catggagcac accgtgctcg cccagcaggc gcaacatgcg gccctcgccg cccagcactt
     2581 ccacgcgcac actcgccggt tcgccctcgt gcagcagcac ccggtcgccg ggcagcgccc
     2641 gcccgtgcgg cgtgtgcgcc tccagcagca actgcgggcc ggtgggatcg gcggagatga
     2701 acacgtgccc ggcgcggatc ccggccagga tggcggggat cgacagttcc tcggcataga
     2761 cccaggtggt gggattgccg atgagatacg ggtaggggtg atccggcgtg ctgatgcggt
     2821 gcagatcgct gccccccacc gccgtggggt aatggccgga gtccagcaag ccctcccaca
     2881 aactaagggt gacatagttg gacaggaacc agggtgcctg ccaggcttca aaggcgtcgg
     2941 tctccgggaa agcggggaac cgccagtcgg ggccgtcctc cttggggtgg ttgatgctgg
     3001 tcagcgcccc gcgcgcccgc gccgccgcga tgatctgttc catacccgtc aggtcccggc
     3061 agcggaagtc gatccagtcg tcggcgtcga gtccccagac gttcaggtgg ccccacggcg
     3121 ttgtcacctc ttcgccgggc agccacagga aatcgggatg cgcgccaagg gccagttcgg
     3181 gattgaccag gttgtggtcg gtcatcgcgc cgaagtcgag tccccagcgg cggtgttcgg
     3241 cggccatctc ggccaccgag ttgtcgccgt cgctatggta agtgtgggcg tggagattgc
     3301 cccgatacca gcgcgcgccg cgctccagca cgcccggctc atagcgcggg tagggcaccg
     3361 gcgccggcac atcctcggcc cgcgcagcca ccgtgcaggt gacggtcacg gtgtaggcgc
     3421 agccgcccgc cgtcacctcc tccggcccca gcatgatgtg ccactccccc gcgggtagcg
     3481 ggccgcgccg gtagcctggc gttgcttcgt taggggccag gaagaagtcc tgccggaa
//
LOCUS       DASUYC010000979         2658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_29507,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000979 DASUYC010000000
VERSION     DASUYC010000979.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29507"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(508..1755)
                     /locus_tag="VF276_16045"
     CDS             complement(508..1755)
                     /locus_tag="VF276_16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020540716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608413.1"
                     /translation="MPGATDTGDLGGTLAADEAGALAELTGLLQAAGCLPAGAPIPLV
                     PRDELGRHRIATCVENQAVVALALRYCDLPTLPDALGRLTGLQHLDLAGNQLAALPAA
                     VGNLRQLQRLYLEENRLTALPEWLGDLGRLEALYLNANALAALPGSIARLAHLRTLNL
                     YGNHLAAVPAGLGRLAGLADLSLGHNRLTTLPATFWGLTGLEVLNLAENGLTELPEGI
                     GNLTWLHTLDAGHNQLAALPDALGELPHLSGFLYLSDNRLATLPRSLANLRELRYLNL
                     THNRFTALPEQIGKLTGLIELRVYNNALVTLPEALGRLADLRELHLADNVLAALPESL
                     GYLTRLAHLDLRNNRLTALPESVGRLAGLTFLDLRNNRLRALPAGLGTLPHLTKLDLR
                     WNQLAARPAWLPALEQRGCLVYE"
     gene            2094..2465
                     /locus_tag="VF276_16050"
     CDS             2094..2465
                     /locus_tag="VF276_16050"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX6608414.1"
                     /translation="MAATEPEHIQVQHILVGFQGSVPGKPIRRSKDEAKTLAYQLLEQ
                     AQSGADFDALVRRHTDDSPPGIYGMSNRGVAPARGEYGRDQMVPAFGNAGFPLAVGEV
                     GIADYDPRASPYGWHIVKRLK"
     gene            2474..>2658
                     /locus_tag="VF276_16055"
     CDS             2474..>2658
                     /locus_tag="VF276_16055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608415.1"
                     /translation="MTTSDLDPPATQPDQDVARLRARVAALEDTAARLRASEARYRSL
                     VLATAQIVWTTDPHGAVV"
BASE COUNT          428 a          915 c          901 g          414 t
ORIGIN      
        1 cgatagtcag cgtcactttg tcgccggacg ccgggggctg cgccgccgtg gtcggcgcgg
       61 tggccgtggt cggctgcgcg gcgggggcca cggcggtcgg cgtggcggct tccccgccgc
      121 acgcggccag caacaagatc atgatgctca gcatgctgag cagccctagt ttgcgcattc
      181 tctctcctcc tcgttaatgg tagcctgtaa cccggctgat cgcgatacat ggaaccgatg
      241 gacgagcgtg tgcggtcgaa tccctcctcc ccatccccgg cggggcaacg gacgggcggc
      301 gctgccgcac cctgggcacg gacggcgagg cggtcagaac tttgttaatc cgatggacta
      361 acaaagttca gtatagcacg gcccgcaaaa ccttgtcaat aggtcccggc taacctcttt
      421 cccggcccca ccccgccgcg gggaggggag tctgctgttt cccccttccc gctccgggaa
      481 gggggcaggg ggttaggccc gccgccacta ctcgtatacc aggcacccgc gctgttccag
      541 ggccggcagc caggccgggc gggctgcaag ctggttccag cgcaggtcga gcttggtcag
      601 gtgcggcagg gtgcccaggc ccgccgggag cgcgcgcaag cggttgttgc gcaggtcgag
      661 aaacgtgagc ccggccagcc gccccacgga ctccggcagc gccgtcagcc gattgttgcg
      721 cagatcgaga tgcgccagcc gggtcaagta gcccagcgac tccggcaggg ccgccagcac
      781 attgtccgcc aggtgcaact cacggagatc ggccagccgg cccagcgcct cgggtagcgt
      841 gaccagggcg ttattataca cccgcaactc aatgagcccc gtcaacttgc cgatctgctc
      901 gggcagcgcc gtgaaccggt tatgcgtgag gttcaggtag cgcagttcgc gcagattcgc
      961 cagcgaccgg ggcaacgtcg ccagccggtt gtcgctcaga taaaggaagc cgctcagatg
     1021 gggcagttcg cccagggcgt cgggcagcgc cgccaactgg ttgtgccccg cgtcgagcgt
     1081 atgcagccag gtcagattgc cgatgccttc gggtagctcg gtcaggccgt tttcggccag
     1141 gttcaacacc tccagcccgg tgagccccca gaaggtcgcc ggtagcgtgg tcagccgatt
     1201 gtgccccagg ctcaggtcgg ccagcccggc taaccggccc agcccggcgg gcacggcggc
     1261 caggtggttg ccgtagaggt tcaacgtccg caggtgcgcc agccgcgcga tgctgccggg
     1321 cagcgccgcg agcgcgttcg cgttgagata gagcgcttcg agtcgcccca ggtcgcccag
     1381 ccattcgggc agcgcggtca gccggttctc ctccaggtac aggcgctgga gctgccgcag
     1441 gttgcctacc gccgcgggta gggcagccag ttgattgccc gccaggtcca ggtgctgcaa
     1501 cccggtcagc cggcccaggg cgtcggggag cgtgggcagg tcgcaatagc gcagggccag
     1561 cgcgaccacg gcctggttct ccacgcacgt ggcgatgcgg tggcgcccca gttcgtcccg
     1621 cggcacgagg ggaatgggtg cgcccgcggg caggcatccg gcagcctgga gcaggccggt
     1681 gagttcggcc agcgcgccgg cttcgtccgc tgcgagtgtc ccgccgagat ctcccgtgtc
     1741 cgtcgcgccc ggcatggtgc cctccccact gcgtgcgctc gtcgcccggc cccgcccccg
     1801 gtagcggcgg gtagcggcaa gtatacaccg atccggtccg cccggcaagc gggtctttcc
     1861 ggcgcaccgc ggcggcttat gtattcaccg cgaagacgcg aaggacgcga agaacgagac
     1921 tgacattaag aatcttcgcg ttcttcgttc cttcgcggtg aattatttga accgctacag
     1981 actctgcgcc actgcggtgc ggattcgccc ttgtttcagc ctgcccggat gtgataaaat
     2041 cgggcaatga ctggccccca agtagggaag gaaagcaggg aaggagttcg cgcatggccg
     2101 ctaccgaacc cgaacacatc caggtgcagc atatcctcgt cggcttccag ggcagcgtgc
     2161 ccggcaagcc gatccgccgc agcaaagacg aagcgaagac gctggcctac cagctactgg
     2221 aacaggcgca aagcggggcc gatttcgacg ccctggtgcg ccggcacacc gacgactcgc
     2281 cgccgggcat ctacggcatg tccaaccgcg gcgtggcccc ggctcgcggc gagtatgggc
     2341 gtgaccagat ggtgcccgcc ttcggcaatg ccggcttccc gctggccgtg ggcgaggtcg
     2401 gcattgcgga ctatgacccg cgtgccagcc cctacggctg gcacatcgtc aagcgcctga
     2461 aataacccgg cccgtgacca catcggacct cgacccgccc gccacgcagc cggatcagga
     2521 cgttgcccgg ctgcgggcgc gcgtggccgc gctcgaagac accgctgccc gcctgcgcgc
     2581 gagcgaagcc cgctatcgct cgctggtact ggccaccgcg cagatcgtct ggaccaccga
     2641 cccccacggc gccgtggt
//
LOCUS       DASUYC010000980         3514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_2011839, whole genome shotgun sequence.
ACCESSION   DASUYC010000980 DASUYC010000000
VERSION     DASUYC010000980.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2011839"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            283..609
                     /locus_tag="VF276_16060"
     CDS             283..609
                     /locus_tag="VF276_16060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608416.1"
                     /translation="MHASIRRYEYAGAPLPVDELVQVGREIAAHLSQAPGFVSFLILS
                     AARQGPGPDMAPNQGLATVSIFEDQPGLEQADRVAAALLDERLAAGCRQQAGITRGEI
                     VFQRGL"
     gene            670..1794
                     /locus_tag="VF276_16065"
     CDS             670..1794
                     /locus_tag="VF276_16065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608417.1"
                     /translation="MNMEPVTGAAPVNMGREASAQVSAGASSARLYAPSWLDQTTRWI
                     RRLPMPAWLFYLLLILIFVFVETVPKWLDGTYPVGVLVPFHVVFAATFAFTLAAHRYL
                     IDSVGTVKPAFYTVFDGDETKLESLLYQLTTSPARPTLLWSLGAAGIASLCWVVLGDS
                     LPRYVLLSTSPLAATVDYLMMIVTWALIAATASYCVHLIQVLNYYLVRRANIDIFKLG
                     SLYAFSGLTGRVAVIFLLGNYIWYLTDPAPPDLSVWRAWRLVTTLAVALIAVGAFLCP
                     LIGVHHVLELQKRRLLEQNAQRVRVVMSRVYHLLDSGESGDLVALKNATDSLVAEQNT
                     LEKLPTWPWHADTLRLVSTVLVLPLLAYLIERILAAVIGH"
     gene            1850..3178
                     /locus_tag="VF276_16070"
     CDS             1850..3178
                     /locus_tag="VF276_16070"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HEX6608418.1"
                     /translation="MEFDWVWVVIRFLVSCVVLTYALIGAGFWTLILRKVGARMQQRL
                     GPNRVGPGGMFQWAADLLKMLLKEPILPAAADKPVFLAAPIVAVFAATAAYAFIPFGE
                     QITVFGRPIPYYVAETPVGLLALLALSSLQVYGLLMAAWSSNSKYPMLSGLRAGAQVI
                     SYEIIMGLALVGVMLLAQSLNLNDISRAQGGSLVGGGAGADTPGIWLLLLQPVGLLIY
                     LLAGLAEVTYPPFDVVEAETELIAGYHVEYSGIQFGLFQAAEFVGTMTVGAITTTCFL
                     GGWQPILPLGGAALAPFWFLLKTSAIVFVLMWVRWTLPRFRYDQLMGICWKVMLPLAL
                     ANLLLVATLRLLVPAGTPPLGHGSFAQALPWLLFSAFELIFAVLVVLALSRQAARSWS
                     GKSEHLRAAGPPAAATARLPEPVAPPRRVELRPGLLTGMGTPDRSPGEGD"
BASE COUNT          540 a         1152 c         1156 g          666 t
ORIGIN      
        1 gaatcgggtg ccctttgtgg agtgggcgcg tcaaagagcc agttggccgg ccagcagcga
       61 actgaacgcg cactgaaggc tactcgatcg attttcagtt gcgcatctca aatatggact
      121 gggtttcgcc ggcggatccc aaaaagatat agcgcgcgcc tataacgccg tgcgatggca
      181 aacgcgggcg gtggcgctat gatgagggcc ggacgcaccg cggtgcgccc gcaggcgcag
      241 ggcacctgcg tcaaggggtg ggtagagagg cacagcaagc cgatgcacgc cagtattcgc
      301 cgctacgaat atgccggagc gccgctgccg gtggacgagt tggtccaggt gggtcgggag
      361 atcgccgccc acctcagcca ggcgccgggg ttcgtctcgt ttctgatcct ctccgccgcc
      421 cgccaggggc cgggtcccga catggcgccg aatcaggggt tggcaaccgt gagcatcttc
      481 gaagaccagc ccggtctgga gcaggcggac cgggtagccg cggccctact cgacgagcgc
      541 ctggcggcgg ggtgccgcca gcaggccggg atcacccgtg gggagatcgt cttccagcgc
      601 ggactgtgaa aggcgcccag gggagcgggg ccttgaggcg ggccggatgg ccgcatggag
      661 ggaacagcca tgaatatgga accggttacc ggcgctgcgc ctgtcaatat gggccgggag
      721 gcaagtgcgc aggtgtccgc gggcgcgagt agcgcgcggc tctacgcgcc aagctggttg
      781 gaccagacca cacgctggat tcgccggctc cccatgccgg cctggctgtt ctatctgctg
      841 ctgatcctga tcttcgtctt tgtcgagacc gtgcctaaat ggctggatgg gacctatccg
      901 gtaggcgtcc tcgtgccctt ccatgtcgta tttgccgcca ccttcgcctt cactctggcc
      961 gcccaccgct atctgatcga ctcagtgggc acggtgaagc cggcctttta cacggtgttt
     1021 gacggcgacg agaccaagct ggagagcctc ctgtaccaac tcacgaccag cccggcgcgg
     1081 cccacgctgc tgtggagtct cggcgcggcc ggcatcgcct cgctgtgctg ggtggtcctg
     1141 ggcgatagtc tgccgcggta cgtcctgctc agcacgtccc cattggcggc gaccgtggat
     1201 tacctcatga tgatcgtcac gtgggccctg attgcagcca ctgcctccta ctgcgtccac
     1261 ctgatccagg tgctgaacta ctacctggtg cgccgggcga acatcgatat tttcaagctg
     1321 ggatccctct acgccttttc ggggctgacc gggcgcgtcg ccgtcatctt cttgctgggc
     1381 aactacatct ggtacctcac cgatccggcg ccccctgatc tctccgtgtg gcgggcgtgg
     1441 cggctggtga cgactctcgc tgtggcgctt atcgccgtag gggcattcct gtgtccgctg
     1501 attggcgtcc accacgtgct ggaactgcag aaacgccggc tcctggaaca aaacgcgcag
     1561 cgggtgcgcg tcgtcatgag ccgggtgtac cacctgctgg acagcggcga gtccggcgat
     1621 ctggtcgcgc tgaagaatgc gacggacagt ctggtcgcgg agcagaacac gctggagaaa
     1681 ttgccgacct ggccgtggca cgccgatacc ctccgcctgg tcagcaccgt gctcgtgctg
     1741 cccctgctcg cctacctgat cgagcgcatc ctggccgctg ttatcggcca ctagctgtgg
     1801 cggggctccg cttggacgcg gagtgccctg cccaatacga gaggatcata tggaattcga
     1861 ttgggtctgg gtggtgatcc gctttctggt gtcctgcgtg gtgctaacct acgcgctcat
     1921 cggggccggc ttctggacgc tcatcctgcg caaggtcggc gcccgcatgc agcagcggct
     1981 cggtcccaac cgcgtcggcc ccggcggcat gttccaatgg gcagccgact tgctcaagat
     2041 gttgctcaag gagccgatcc tgccggcggc ggcggataag ccggtcttcc tggccgcgcc
     2101 gattgtcgcc gtcttcgccg ccaccgccgc ttacgccttc atccccttcg gtgagcagat
     2161 caccgtcttt ggccgcccca tcccctacta tgtggccgag acgccggtcg gcctgctcgc
     2221 cctcctggcc ctctcctcgt tacaggtcta cggcctcctt atggcggcct ggagctccaa
     2281 cagcaaatac ccgatgctga gcggcctgcg ggccggcgcc caggtgatta gctatgagat
     2341 tatcatgggc ctcgcgctgg tcggggtgat gttgctggcc cagtcgctca acctcaacga
     2401 catcagccgg gctcagggcg gatcgttagt tggcggaggg gccggcgcgg acacgccggg
     2461 tatctggctg cttctgttgc agccggtcgg cctgctgatc tacctgctgg cggggctggc
     2521 agaggtcacc tacccgccgt tcgacgtggt ggaagccgag accgagttga tcgccgggta
     2581 ccatgtggaa tacagcggga tccagttcgg cctgttccag gcggccgagt tcgtcggcac
     2641 catgacggtg ggcgcgatca ccaccacctg cttcctgggc ggctggcagc cgatcctgcc
     2701 gctgggcggc gcggcgctgg cccccttctg gttcctgctc aagacaagcg cgatcgtgtt
     2761 cgtgctgatg tgggtgcgct ggaccctgcc gcgcttccgc tacgaccaac tgatgggcat
     2821 ctgctggaag gtcatgctac cgctggcctt ggcaaacctg ctgttggtcg cgacgctgcg
     2881 cctgctggtg ccggcgggca ccccgccgct gggccacggc agcttcgcgc aggcgttgcc
     2941 gtggctgctg ttctccgcct tcgaactgat cttcgcggtg ctggtggtac tcgcgctgtc
     3001 gcggcaggcg gcgcggtcgt ggtcgggcaa gtctgaacac ctgcgagcag caggcccgcc
     3061 cgctgctgca acggcccgct tgccggagcc cgttgcgcca ccccggcgtg tcgagctccg
     3121 accgggcttg ttgaccggga tggggacccc agaccgctca cccggcgagg gcgactaggt
     3181 aagcttggct gacttgaaac caccggataa tggcggcagc accaacttgg cggaagcagt
     3241 cgctgcgcgg acccaaaccg ctgataaccg aaccacactt tgtcacgaca tgccggctct
     3301 gccacacttt tgatactgcc ggcgaaggcc ggcccctaac tgcacaggct gaccccggtc
     3361 gccattaacc ggctacacgg cgagacggca gtcttcaggc gcgcttgccc caacagccat
     3421 tcgcccaacc gcagcaggtt gggttccgcg gccattagaa cgcggcccac atgagtccgc
     3481 gccgggccaa tataggccgt caccgtgtgc tgcc
//
LOCUS       DASUYC010000981         2614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1317485, whole genome shotgun sequence.
ACCESSION   DASUYC010000981 DASUYC010000000
VERSION     DASUYC010000981.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317485"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1731)
                     /locus_tag="VF276_16075"
     CDS             complement(<1..1731)
                     /locus_tag="VF276_16075"
                     /inference="COORDINATES: protein motif:HMM:NF019317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEX6608419.1"
                     /translation="MPDQVTSSHSTSPPVLAPPRPPDRSATPPSTRPLSLFTVGLIVL
                     ISAILVLPLALPGRVELAAGQPARTDIFAPQYVRYESEVLTAQDRENARLATGPVYES
                     NISLVDSQRRRLNDVLSTIESIRGNTDDLPAAREAQMHQLPNVDLSDADVRTLLQATP
                     SDASRIRSEILRVYDNVMISGKIADTSQLQRQRDDLFTLFSSELTPQQRRAAANVLAP
                     FIAINLILNPEKTEEARQKAAAAVQPRVVEVQKGEIILRVGQIADDAAIEKLEKAGLS
                     NPTVTAANAAAVVGSVMIMLLLLHFYLLRLQPAIWRHRKSLLLLALVLVAPTLIMRVI
                     VPGHAIWPYVLPLAAASMLVAVLLDANLALVITVVLAVFSALLTQGSFDLPFFYLVGG
                     ATAVFAIWRAERVSTFVVSGLYIAIASLAATLLLRVIENQHLDWNALWVLTAAAGING
                     ALSASLTFATFSLLGSWFGIATMLQLLELAHPTQPLLRRLMREAPGTYHHSLVVSNLA
                     EHAAEVVGADPLLARVAGYYHDIGKVLNPYVFIENQSGMGNIHDTLDPVTSARLIRAH
                     ITDGQALAQRY"
     gene            complement(1761..2213)
                     /locus_tag="VF276_16080"
     CDS             complement(1761..2213)
                     /locus_tag="VF276_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018975720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX6608420.1"
                     /translation="MTIEERLMNDLKDAMRAGPAGELRKSTIRMARAAIHNTQIALQH
                     PLSEEEVIAVLQREVKQRRDAVEEYVKAHREDRAQIERDEIAILQQYLPQQMSADEIR
                     AYVEQAIAETGARGPADIGKVMPVLMQRLKGKAEGRLINQLVRQQLEG"
     gene            complement(2377..2559)
                     /gene="rpsU"
                     /locus_tag="VF276_16085"
     CDS             complement(2377..2559)
                     /gene="rpsU"
                     /locus_tag="VF276_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEX6608421.1"
                     /translation="MAEVVVGDHESFESALRRFTKKVQADGILREAKQREHYEKPSAK
                     RKRKAAARLRKSAKSR"
BASE COUNT          419 a          820 c          899 g          476 t
ORIGIN      
        1 gtagcgctgg gccagggcct gcccgtcggt gatatgcgcg cggatcagcc gcgcgctcgt
       61 caccgggtcg agcgtgtcgt ggatgttgcc catgcccgac tggttctcga taaagacata
      121 cggattgagg accttgccga tgtcgtggta atagccggcc acgcgggcca ggagcggatc
      181 ggcgccgacg acctccgccg cgtgctcggc caggttgctc accaccagac tgtggtggta
      241 ggtgccgggc gcctcgcgca tcaggcggcg cagcaacggc tgcgtcgggt gggccagttc
      301 gagaagctgg agcatagtgg cgatgccgaa ccagctcccc agcaggctga aggtggcgaa
      361 ggtcaggctg gccgatagcg cgccgttgat gcccgccgcc gccgtcagca cccacagggc
      421 gttccagtcc aggtgctggt tctcgataac ccgcagcagc agcgtggcgg cgagcgatgc
      481 gatcgcgata tagaggcccg aaacgacaaa ggtgctgacg cgctcggcgc gccagatggc
      541 gaagacggca gtagccccgc ccaccaggta gaaaaacggc agatcaaagc taccctgggt
      601 cagcagcgcg ctgaacacgg ccagcaccac ggtaatgacc agggccaggt tggcgtccag
      661 cagcacggcc accagcatac tggcggcagc cagcggcagc acatacggcc agatggcgtg
      721 ccccggcacg atcacgcgca taatcagggt gggcgcgacg agcaccaggg ccagcagcag
      781 caacgacttg cggtgccgcc agatagcggg ctgcaagcgc agcaggtaga aatgcagcag
      841 caagagcatg atcataaccg agccgaccac agcagcggcg ttggccgccg tcaccgtcgg
      901 gttgctcaaa ccggcttttt ctagcttctc gatggcggcg tcgtccgcga tctgcccgac
      961 gcgcaggatg atctcgccct tctgcacctc caccacgcgc ggttgtaccg ccgccgcggc
     1021 tttctggcgg gcttcttcgg tcttttccgg gttcagaatg aggttgatgg cgatgaatgg
     1081 ggccagcacg ttcgctgcgg cgcgccgttg ctggggggtg agttccgaac tgaagagcgt
     1141 gaacaggtcg tcacgctggc gttggagctg gctggtgtcg gcgattttgc cgctgatcat
     1201 cacgttgtcg tagacgcgca ggatttcgct gcggatgcgg ctggcgtcgc tcggcgtggc
     1261 ttgcagcagg gtgcggacgt cggcgtcgct caggtccacg ttgggcaact ggtgcatctg
     1321 cgcctcgcgg gccgccggca ggtcgtcggt attgccgcgg atgctctcga tggtcgagag
     1381 cacgtcgttg aggcgccggc gctggctgtc aaccaggctg atgttggact catagaccgg
     1441 tccggtcgcc aggcgggcat tttcccgatc ctgggcggtg aggacttcgc tctcgtagcg
     1501 gacgtactgc ggggcgaaaa tatcggtgcg ggcgggctgg ccggcggcca gttccacccg
     1561 gcccggcagg gccagcggca acaccaggat ggcgctgatc aggacgatca ggcccacggt
     1621 aaacaggctg agcgggcggg tgctcggcgg ggtggcggat cggtcgggag gccggggcgg
     1681 ggcgaggacg ggtggggatg tcgagtggct cgacgtaact tgatcgggca cagcgcctcc
     1741 tgttggcggc ccgcgggccg ctagccttcg agttgctggc gcaccaactg attgatcagc
     1801 cggccctcgg ccttcccttt cagccgctgc atcagcaccg gcatcacctt gccgatgtcc
     1861 gcaggcccgc gggcaccggt ctcggcgatg gcctgctcca cgtaggcccg gatctcgtcg
     1921 gcgctcattt gctgcggcag gtactgctgc aggatggcga tttcatcgcg ctcaatttgc
     1981 gcccggtctt cccgatgggc cttgacgtat tcctcgacgg catcccgtcg ctgcttgacc
     2041 tcgcgctgca gcaccgcgat gacttcctcc tcgctcaagg ggtgctgcag ggcgatctgg
     2101 gtattgtgga tcgcggcgcg cgccatgcga atagtggact tgcgtagctc acccgccggt
     2161 ccggcccgca tggcgtcctt caaatcgttc atcaaccgtt cttcaatggt catggcgctg
     2221 tccgtacctg ctatgttcag taatatcttc cggaaggtgg atcgggagcc gggtatacaa
     2281 aagggttaga agcggcgccc gccgctggaa gcgccccgcc gcggagagcg ggggtgcttc
     2341 gggtgggggc gcggggcttc taaccctggg caatacctag cggctcttcg cgctcttgcg
     2401 caggcgggca gcggctttcc gcttgcgctt ggcgctgggc ttctcgtagt gctcgcgctg
     2461 cttcgcctcg cgcagaatgc cgtccgcctg caccttcttg gtgaagcggc gcaacgcgct
     2521 ctcgaagctt tcgtgatccc caacgacaac ttcagccatc tctgtcccct ccttcccgcc
     2581 ggcgccatca tctacccttg agcaaaacag ggca
//
LOCUS       DASUYC010000982         2000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1317837, whole genome shotgun sequence.
ACCESSION   DASUYC010000982 DASUYC010000000
VERSION     DASUYC010000982.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317837"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1216
                     /locus_tag="VF276_16090"
     CDS             <1..1216
                     /locus_tag="VF276_16090"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6608422.1"
                     /translation="GLGHLALYAGDLDTARTRYEEALTLRREIGNKRGAASALESLGD
                     VAAQQGDYAQAIALCEESLALSRAIGDNWGVVHALAGIAGACANRGDYARATTLMEES
                     LTLAQAACSRSMIAALLGGLGQLAARQGDYARAATLLDESLGMFREMEYRVHLCEVLT
                     LRGSVALRQNDFDQAGALLEESLALARSLRNDECSCKALRALGRLALRQGQPGRARTL
                     LEETVALHRRLPCRYDLALACDGLAMACAADGAAERAARLFGAAQALRDAISVPIIPA
                     DQAEHDQYLAATQAALGDAAFAAAWTAGQTLPPDALLEGEESGAGTESTGAPAANGGN
                     GARLAHPAGLTAREIEVLRLVAGGLTDAQVAERLTLSPRTVSSHLYSIYSKLGVTSRT
                     AATRFALEHALL"
     gene            1430..1828
                     /locus_tag="VF276_16095"
     CDS             1430..1828
                     /locus_tag="VF276_16095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608423.1"
                     /translation="MYLNQSLIGEVAGAQHADRLREANAARRARQIRPARPAGHLRHA
                     FAALQGWAGLSADKPSHEYPIREVETTIARCIRQTPPAAESPADLDEALVQQLLAGYA
                     HPVSEPPPGTSYQAEWLPDHQSNPYLRDAA"
BASE COUNT          314 a          748 c          663 g          275 t
ORIGIN      
        1 gggcctgggc cacctggcgc tgtacgcagg cgacctggac accgcccgca cccgctatga
       61 agaagcgctg actctgcgcc gcgagatcgg caacaaacgc ggggcggcga gcgcgctgga
      121 gagcctgggc gacgtggcgg cgcagcaggg cgattatgcg caggcgattg ccctgtgtga
      181 agagagcctg gccctctcgc gcgcaatcgg cgacaactgg ggtgtcgtcc acgcgctggc
      241 cgggatcgcg ggggcttgcg ccaaccgcgg ggactacgcc cgcgccacca cgctgatgga
      301 ggaaagcctg acgctggccc aggcggcgtg ttcgcgcagc atgattgccg cgctacttgg
      361 cgggttgggc caactggccg cccggcaggg tgactacgcc cgcgccgcga ccctgctcga
      421 cgagagcctg ggcatgttcc gcgaaatgga gtatcgcgtc catctctgcg aagtcctcac
      481 cttgcgcggc tccgtggcgc tgcgacagaa cgactttgac caggccggcg cgttgctcga
      541 agagagcctg gcgctggccc gcagcctgcg caacgacgag tgcagttgca aagccctgcg
      601 cgcattgggc cggctggcgc tgcgccaggg ccagcctggg cgggcgcgca cgctgctcga
      661 agaaacggtg gccctccatc gccggctccc ctgccggtac gacctcgctc tggcctgcga
      721 cgggctggcg atggcctgcg cggcggacgg cgccgccgag cgggccgcgc ggctcttcgg
      781 cgccgcccag gcgctgcgcg atgcgatcag cgtcccgatc atccccgcgg accaggccga
      841 gcacgaccag tacctggcag ccacccaggc cgcgctcggc gacgccgcgt tcgccgccgc
      901 ctggaccgcg ggccagaccc tgccgccgga tgcgctgttg gagggcgaag agtcgggtgc
      961 cggcacggaa tcgacgggcg ccccggcagc caacgggggc aacggcgccc gcctggccca
     1021 cccggcgggc ctcaccgcga gggaaatcga ggtattgcgg ctcgtggcgg gcggcctgac
     1081 cgacgcgcag gtggccgaac gcctcaccct gagcccgcgc accgtcagtt cgcacctcta
     1141 ctcgatctac agcaagctcg gcgtcacgtc gcgcaccgcc gccacgcgct tcgcgctcga
     1201 acacgccctg ctctgatccc tgcgcccccg gcaacgtcct ccttccggca gccgtctcag
     1261 taattctact gactccgcat acattactgg tcccatcgca cccgcccaat ttcagtaagc
     1321 aggttgggaa attctgctga tgtgcgccgc cgcgccgtgc gctacactgg taccacaacg
     1381 acagtcgcca gttgagcatc cggcagccca cgccgaagga gaacgtccta tgtacctcaa
     1441 tcaatcgctc atcggcgagg tagccggcgc ccagcacgcg gaccgtttgc gcgaagcgaa
     1501 cgccgcccgc cgggcgcgcc agatccgccc ggcgcgcccc gcaggccacc tgcgccatgc
     1561 tttcgccgcc ctccagggct gggccgggct ctctgcggac aagcccagcc atgaatatcc
     1621 catccgggaa gtcgagacga ctattgcccg ctgcatacgc caaacgccgc ccgcggcgga
     1681 atcgccggcc gatctggacg aggcgctggt tcagcagttg ctggccgggt acgcgcaccc
     1741 tgtatcggag ccgccgcccg gcaccagcta tcaagccgaa tggcttcctg accaccagag
     1801 caacccctat ctgcgcgacg cggcctgatc ccgccgaggg tagcgccgcc cgctagagca
     1861 cgactccccg accaccgtcc tgagcgcctc gccggcgagg gatacgccaa tgccgcagca
     1921 aggccgcaca gcgtacgccg cggcgccgcg gacggttcgg gagccgcgcc ggctgttgcg
     1981 cccctactca cgtccattcc
//
LOCUS       DASUYC010000983         4073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1317923, whole genome shotgun sequence.
ACCESSION   DASUYC010000983 DASUYC010000000
VERSION     DASUYC010000983.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317923"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VF276_16100"
     CDS             <1..205
                     /locus_tag="VF276_16100"
                     /inference="COORDINATES: protein motif:HMM:NF018933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX6608424.1"
                     /translation="TCPACGGQDKQNRRTQSKFLCVACGFAGLADHIAAMNIRSRAAV
                     NRPDFAVGTLLGTQAPSAESSTC"
     gene            complement(218..1138)
                     /locus_tag="VF276_16105"
     CDS             complement(218..1138)
                     /locus_tag="VF276_16105"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX6608425.1"
                     /translation="MALTLGKRKPAGAGPRRGSFTENLVFLVPMLFFVVLLYGTLLWT
                     LYVSFTNWHNITPNYDLSGFQWYEFLVQQPRFQVDITNNLLWLVVGVVPTIVLAILLA
                     YFLELMPFPKIESYIRTLILYPVAMSFIVTGTIWSWMYEPQKGVLNTLLRGVGLDPPF
                     RYTTDPNSATLWLIVIFIWQYLGFSVIIMQSAFRTSDLQEMVESATIDGASRLRILVS
                     ILLPNMRGGILVLVSLLLISTLKVFDIVFIVTYGGPGISTDVLALNMWVTTFQQHLVS
                     AGAGIGIVIFLMAFVIVIPYTIYAFRKWFE"
     gene            complement(1282..2652)
                     /locus_tag="VF276_16110"
     CDS             complement(1282..2652)
                     /locus_tag="VF276_16110"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6608426.1"
                     /translation="MSRRVLGVLASLVVLSLLLSACGTEAAPTATAVPSSGSTSGQTG
                     AKTGGERKLEIFHWWTAPGEREAADAMFKTLKDKYPDIELVENPNPGGGGVNQRVVLQ
                     SRIAAGDYPDTFQTLGGAELKNYVDSNVLQPLDDLWKEIDYAAVIPGPLAKAVTVNGH
                     PYSVPLNMHIQNILYYNKKLFDELSIQPPQTFEDLVAACDKIKSSKPDMTCLGLGSKE
                     KWGDAFVFDSILLQEGGADNYVKLYKGEIDVTSDKVYRSALEKFQKLAPYVNSDHAGL
                     TWDQAVALVGSGKAAMTIMGTWAIGAFTKGSNWKPGEDFGAVTFPQKPERILLFHPDT
                     YGITTKAPHPGATADWLKVVASSELQVPTDVIQGGLFARKDIDPSKFPDPIRQELAGY
                     VRDNPGKLILDQHGSILPATAQPVYWDILSSFLVQPNVDKAIKDVDSMFKTYKVKDAS
                     AWYAWP"
     gene            complement(3123..>4073)
                     /locus_tag="VF276_16115"
     CDS             complement(3123..>4073)
                     /locus_tag="VF276_16115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608427.1"
                     /translation="APLAAAQASQAQAPAEPDHSAGAQFRRGLELLAEGTREPGGDRT
                     LLIVEPEAGQAATAAFNAAAAADPGAAIYRYFVGVARRYGEGYPAAQGEFDRARQIDP
                     GYWEAQMQSFFGPRWHDAFAYPAWSARAAALTGVLRALLPPRPGSRLVIVREGGARTV
                     SVLCHTRRDSWKRPPTADMPARIELVPSATPSGLIVALYVALRDDPQNPYRGETFINP
                     AEAPGPDDDATQLGQNLILQLARQSHTYLIFADETGTLLLNRQLVFDAATRHNLDQVA
                     TQVQAVAYQAIERNQPMSLGQFQEAARWHIQQFPLERVQI"
BASE COUNT          719 a         1227 c         1364 g          763 t
ORIGIN      
        1 gacgtgccct gcctgtggcg ggcaggacaa gcaaaaccgt cgaactcaaa gcaaattcct
       61 ctgtgtggcg tgtggcttcg ctgggctagc cgaccacatc gcggctatga atattcgtag
      121 ccgggccgct gtaaaccggc cagacttcgc ggtgggtact ctgcttggta cgcaggctcc
      181 gtccgcggaa agctccactt gttagagtgc gagttgttta ctcgaaccac ttcctgaatg
      241 cgtagatggt gtacgggatg acaatcacaa aagccatgag gaagatgacg atgccgatcc
      301 cggccccggc ggagaccagg tgttgctgga atgtcgtcac ccacatgttg agcgccagca
      361 cgtcggtgga gatgcccggc ccgccgtagg tgacgatgaa cacaatgtcg aacaccttga
      421 gcgtggagat cagcagcagc gacaccagca cgagaatccc gccgcgcatg ttggggagga
      481 ggatcgagac caggatgcgc agccgcgagg ccccgtcgat ggtggccgat tcgaccatct
      541 cctgcagatc gctcgtgcgg aacgccgact gcatgatgat caccgagaac cctaggtatt
      601 gccagataaa gatgacgatc aaccataggg tcgcgctgtt ggggtccgtc gtatagcgaa
      661 acgggggatc cagccccacg ccccgcagca gggtgttgag cacgcctttt tgcggttcgt
      721 acatccacga ccagatagtg ccggtcacga tgaacgacat cgccaccggg tagaggatca
      781 gggtccggat gtaggactcg atcttgggaa aaggcatcag ctccaggaag taggccagca
      841 ggatggcaag cacgatggtg ggcaccaccc cgaccaccag ccagagcagg ttgttggtga
      901 tgtccacctg gaagcgcggc tgctgcacca ggaactcgta ccactggaat ccgctcagat
      961 cgtaattggg ggtgatgtta tgccagttgg tgaacgagac gtacaatgtc cacagcaagg
     1021 taccgtagag cagcaccaca aagaacagca tgggaacgag aaagaccagg ttttccgtga
     1081 agctgccgcg gcgcgggccc gcgcctgccg gcttgcgctt tcccagggtc agtgccatag
     1141 ctcggttatc cttcgcgccg ggcgcaaaga caaaaagcct gtcgggcgca agcgaagggg
     1201 cgaaagacga ctgcgcgggt tcgtccttcg tctttcgccc ttcgccgcgt ggctaattgc
     1261 cggctgagat tcgctggaag attacggcca ggcgtaccag gccgaggcgt ccttgacctt
     1321 gtaggtcttg aacatgctgt ctacatcctt gatcgccttg tccacgttgg gttgcacgag
     1381 gaaggaggac aggatgtccc agtagaccgg ttgggccgtg gccggcaaga tgctgccgtg
     1441 ctgatcgagg atcagcttgc cgggattgtc gcgcacataa ccggccagct cctggcggat
     1501 cggatccggg aacttgctcg ggtcgatgtc cttgcgggcg aacaggccgc cctggatcac
     1561 gtccgttgga acctgcagtt cggacgaggc caccaccttc agccagtccg ccgtggcccc
     1621 cggatgcggg gccttggtgg tgatgccgta ggtatcgggg tggaacagca ggatgcgctc
     1681 gggcttctgc gggaaggtga cggcgccgaa gtcctcacca ggcttccagt tcgagccctt
     1741 ggtaaacgcg ccgatggccc aggtgcccat gatggtcatg gccgctttac ccgacccgac
     1801 cagggccacc gcctggtccc acgtcaggcc ggcatggtcc gagttgacgt acggggccag
     1861 tttctggaac ttctccagcg cgctgcggta gaccttgtcg ctggtcacgt cgatctcgcc
     1921 cttgtacagc ttgacgtagt tgtccgcgcc gccctcctgc agcagaatgc tgtcgaagac
     1981 gaacgcatcg ccccacttct ccttggaccc gagaccaagg caggtcatat cgggcttgga
     2041 ggacttgatc ttatcgcagg cggcgacgag gtcttcaaag gtctgcggcg gttggatgct
     2101 cagttcatcg aacagcttct tgttgtaata caggatgttc tggatgtgca tgttcagcgg
     2161 gacgctgtag gggtgcccgt tgaccgtgac tgccttggcg aggggaccgg ggatgacggc
     2221 ggcgtagtcg atctccttcc acaggtcatc cagcggctgc aacacattgc tgtcgacgta
     2281 attcttcagt tcggcgccgc cgagggtctg gaaggtgtcg ggatagtcac cggcggcaat
     2341 gcgcgactgc aagaccacgc gctggttcac gccgcccccg ccgggattgg ggttttcgac
     2401 cagttcgata tcgggatact tgtccttcag ggtcttgaac atggcgtcgg cggcttcgcg
     2461 ctcgccgggc gccgtccacc agtggaagat ttcgagcttg cgttcgccgc ccgttttcgc
     2521 ccccgtttgc ccgctcgtgg atccggaact agggaccgcg gtggcggtag gggccgcctc
     2581 ggtgccgcac gcgcttagta gcagactgag caccacgagg gatgccagca cacccagcac
     2641 tctccgagac ataggagatc ctccttcggt ttctatgcgc atgtgtgatg aacaagacaa
     2701 caatctgagg ctatcgttgt tccggcgcca ggtacttacc tccttcctat cgcatatcca
     2761 gcaaccatgc ggcggcccca ggaggtgcgg gctgccccgg cagtctgtaa gccgggctgg
     2821 ggtggcccct ggggtcctgt ggtacgcgct gcacagtttc taacatttgc gcctggttgt
     2881 caatagccgt ccgccattct acacccgata cgtaaatatt ctcgtagaat agggtacacg
     2941 aatcgttaca agtccggcca gggctgaggg gatggtttgc ggcgcacgcg attcgccgga
     3001 agtcagcgcg atccggcttt cgcgtcgcgg ccgcgcgctg gaggacccgg caaaagggcc
     3061 gggggcgcgg aacctgtttc cgcacccccg gtttgtctct gcttgcagtg gccgggcccg
     3121 gttcagatct gcacgcgctc cagcgggaac tgctggatgt gccagcgggc ggcttcctgg
     3181 aactggccca gcgacatcgg ctggttgcgc tcgatggcct ggtaggccac cgcctgcacc
     3241 tgggtcgcca cctgatccag attgtggcgc gtggcggcgt caaagacgag ctggcggttc
     3301 agcagcagag tgcccgtctc gtcggcgaag atgagatagg tgtggctctg gcgggccagt
     3361 tgcaggatca ggttctggcc cagttgcgtg gcgtcgtcgt cggggccggg cgcctcggcg
     3421 ggattgatga aggtctcgcc gcggtagggg ttctgtggat cgtcgcgcag ggctacgtag
     3481 agggcgacga tcagcccgct gggcgtggcg ctgggcacca gttcgatgcg cgcgggcatg
     3541 tcggcagtgg gcgggcgctt ccagctatcg cgccgggtgt ggcagagcac gctcacggtg
     3601 cgcgcgccgc cctcgcgcac gatcaccagg cggctgccgg ggcgcggcgg cagcaacgcg
     3661 cgcagcacgc ccgtcaaagc cgcggcgcgc gccgaccacg ccgggtaggc aaacgcatcg
     3721 tgccagcgcg gaccgaagaa gctctgcatc tgcgcttccc agtagccggg gtcgatctgc
     3781 cgcgcccggt cgaactcgcc ctgcgccgcc gggtaaccct cgccatagcg gcgggccacc
     3841 ccgacaaagt agcgatagat ggccgcgcca gggtcggcag cggcggcggc gttgaaggcg
     3901 gcggtggcgg cctgcccggc ttcgggttcg acgatcagca gcgtgcggtc gccgcccggt
     3961 tcgcgcgtgc cctcggccag caactccagg ccccggcgga attgcgcgcc cgcgctatga
     4021 tccggttccg cgggggcctg cgcctgtgat gcttgcgcgg cggccagggg cgc
//
LOCUS       DASUYC010000984         4216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_626514,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000984 DASUYC010000000
VERSION     DASUYC010000984.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626514"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(253..750)
                     /locus_tag="VF276_16120"
     CDS             complement(253..750)
                     /locus_tag="VF276_16120"
                     /inference="COORDINATES: protein motif:HMM:NF025865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEX6608428.1"
                     /translation="MTLEEAIRTFRAVRQFTDQPLSDEDIQAILNAGRRAQSSKNTQP
                     WQFVAVRNRETLQQLATCGTFAGHLAGAALGVALLSPVEGFYDLGQATAYMQLAAWAR
                     GIGSCIASIYEPEKARAILGVPDDQNFRYALSFGYPAPNPTRPTGLKKGGRLPLEEVV
                     RWEHW"
     gene            complement(778..1938)
                     /locus_tag="VF276_16125"
     CDS             complement(778..1938)
                     /locus_tag="VF276_16125"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX6608429.1"
                     /translation="MPEAVILEAVRTPYGKNRNGAYHATHPVELLAGTLQGLLDRAPL
                     EPGRIDDVVVGTVTQVREQAADIARQAVLLAGWPVEVPAVTINRFCGSSQEAVHMAAR
                     AVEAGDMRYAVGGGVESMSRVPMFSDLGGGLNTLNAALRARYDLVDQGESAERVAEQW
                     HISREESDALARESHRRASVAWHAGCNTELLPTPGHDAAGNPLTLTRDEAVRDVIDPA
                     RMAGLAPVFRPADRGVVTAGNASAIVDGASAVLVGERAVAEADGFRPRARFRARVVVG
                     SDPVLQLSGVIPATRQALDRAGLALADIDWIEINEAFATVVLAWAREFDPDMGRVNPW
                     GGAIAHGHPLGASGAGLMAKMLAGLEATGGQFGLQVMCVGLGMSTATVIERL"
     gene            complement(2008..2886)
                     /locus_tag="VF276_16130"
     CDS             complement(2008..2886)
                     /locus_tag="VF276_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013889343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX6608430.1"
                     /translation="MNWPAKAGPLAGLRVLSLAGNIPGPLAVARLHALGAAVCKVEPP
                     GGDALAAACPAWYAALHAGQEVIRLNLKDRADRAQLDPLLAAADLLITASRPAGIARL
                     GLAWDDLHAAYPDLCQVAIVGHAAPAADRPGHDLTYQVAAGLLVPPALPRVLLADLAG
                     AERAVAVALALLLARQRGQGSGYREVALAEVAEDFAAPLRHGLTAPGGLLGGGLPSYN
                     IYAAQEGWVALAALEPGFVQRLQHSLHIAEITHSALADIFRTQPAQTWEQWAIQHDLP
                     LVAVHDPTANADHPSY"
     gene            complement(2870..4003)
                     /locus_tag="VF276_16135"
     CDS             complement(2870..4003)
                     /locus_tag="VF276_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6608431.1"
                     /translation="MDFQLSAEQRMIRDLAHTFADREIRPVAAEYDRAERFPRPLFER
                     AQELGLLNLTMPEAYGGAGLGGLEQCIVVEQLAWGCVGITAAMTINGLVADALLVGAT
                     PEQQAAYLPRLRTGELGCYALTEPGAGSDVAAITTSARRDGDEYVLNGAKVWISNATE
                     ASFIVLFAKTDPAAGHRGISAFIVERDTPGVTVGHKMGKLGQKAAPTAEIHLDNARVP
                     VSARMGEEGTGFRIAMGVFDRSRPMVAAMGVGVTARCLDEALSYAKTRQTMGRPILEH
                     QAIGHKIAQMGLRLEAARLLTYQAAWLHDSGQRNTLQAAYAKAFAADTAMWAATEAIQ
                     VHGGMGYSTEYPVEKLLRDAKVLQIYEGTSEIQHNIMVRELAR"
BASE COUNT          593 a         1481 c         1460 g          682 t
ORIGIN      
        1 gagccgcccc cgctaataag aaaggtgctg ttctcgactt gcatggcctt ctccctccct
       61 gccccgccgt ccggccgggc agcccgtcac caaccccccg tcattctgag cggagcgaag
      121 aatctccgtc tactaggcta aacgacgaga ctcttcgctg cgctcagcgt gacaaagcta
      181 gggtggtagc cagctcggcc tgaccagcat cgcgtgaata ctatccagtt gctcggctgg
      241 aaagcggcaa aatcaccagt gttcccagcg cacgacctct tccaacggca ggcgcccacc
      301 cttcttgagg ccggtcgggc gggtcggatt gggcgcggga tagccgaagg acagcgcgta
      361 gcggaaattc tggtcgtcgg gcacgcccag gatggcgcgg gccttctccg gctcgtagat
      421 cgaggcgatg cacgagccga tgccgcgcgc ccacgccgcc agttgcatgt aagccgtggc
      481 ctgccccagg tcatagaagc cctcgaccgg cgagagcaac gctacaccca gcgccgcccc
      541 ggccaggtgc ccggcaaaag tgccgcaggt cgccagttgc tgcaacgtct cccggttgcg
      601 cacggcgacg aactgccacg gttgcgtgtt cttcgagctt tgcgcgcgcc gcccggcgtt
      661 caggatcgcc tgaatgtcct cgtcgctcag cggctggtcg gtaaactggc gcaccgcgcg
      721 aaacgtccgg attgcttctt ctaatgtcac cttatcccca ctttctgccg gcatgactca
      781 cagtcgctcg ataaccgtcg ccgtggacat gcccaggccg acacacatca cctgaaggcc
      841 gaactggccg ccggtggcct ccagtcccgc cagcatcttc gccatcaggc ccgcgcccga
      901 cgcgcccagc gggtggccgt gggcgatagc cccgccccac gggttgaccc ggcccatgtc
      961 ggggtcgaac tcgcgcgccc aggccagcac caccgtggcg aacgcctcgt tgatctcgat
     1021 ccagtcaatg tcggccagag ccagcccggc gcggtccagg gcctggcgcg tggccgggat
     1081 gacaccgctg agttgcagca ccgggtcgct gccgaccacc acgcgggcgc ggaagcgggc
     1141 gcgcgggcgg aagccgtcgg cctcggccac agcgcgctcg cccacgagca ctgccgacgc
     1201 gccgtccacg atggcgctgg cgttgcccgc cgtgaccacg ccccgatccg ccgggcggaa
     1261 gacgggcgcc aggccggcca tcctggccgg gtcgatcacg tcgcgcaccg cctcgtcccg
     1321 cgtgagggtg aggggattgc ccgcggcgtc gtggccgggc gtgggcagca gttcggtgtt
     1381 gcagcccgcg tgccacgcga cgctcgcccg gcggtggctc tcgcgcgcca gggcgtccga
     1441 ctcctcgcgg ctgatgtgcc actgctctgc cacccgctcg gcgctctcgc cctggtccac
     1501 caggtcgtag cgggcgcgca gggcggcgtt gagcgtgttc aggccaccgc ccaggtcgct
     1561 gaacatgggc acccggctca tgctctcgac gccgccgccg accgcatagc gcatatcgcc
     1621 cgcctcgacg gcgcgcgccg ccatatgcac cgcctcctgg ctcgacccgc agaagcggtt
     1681 gatcgtcacg gcgggcactt cgaccggcca cccggccagc aagacggctt gccgggcgat
     1741 gtccgccgcc tgctcgcgca cctgggtgac ggtgcccacc acgacgtcgt cgatgcggcc
     1801 tggttccagg ggcgcgcggt ccagcaggcc ctgcaacgtc ccggccagca actctaccgg
     1861 gtgggtggcg tggtacgcgc cgttgcggtt cttgccgtag ggcgtacgca ccgcttcgag
     1921 aattaccgct tcgggcatat cgggttcctt tctggtgacg agccgtcagc cgtcagccgt
     1981 cagccgtcag tggtcggtgg tcagtggtca gtatgacggg tgatcggcgt tggctgttgg
     2041 gtcgtggacg gcgactagcg gaagatcgtg ctggatggcc cactgctccc aggtttgggc
     2101 gggttgcgtg cggaagatgt ccgccagggc gctgtgggtg atctcggcga tgtgcaagga
     2161 gtgttgcagg cgttgcacga agccgggttc cagggccgcc agcgcgaccc agccttcttg
     2221 cgccgcgtag atgttgtagg acggcaggcc gccgcccagc aggccgccgg gcgcggtcag
     2281 cccgtggcgc agcggcgcgg cgaagtcctc tgcgacctcc gccagggcga cctcccggta
     2341 gccgctgccc tggccgcgct gccgggccag cagcagcgcg agcgccacgg cgacggcgcg
     2401 ctccgccccg gctaggtcgg ccagcagtac gcggggcagg gctggcggga cgagcaggcc
     2461 tgccgccacc tgataggtca ggtcgtggcc ggggcgatct gctgccgggg ccgcgtggcc
     2521 gacaatcgca acctggcaga ggtcgggata ggcagcgtgc aggtcatccc aggccagccc
     2581 caggcgggcg atgccggcgg gccgcgacgc cgtgatcagc agatcggcgg cggccagcag
     2641 cggatcgagt tgggcgcggt ccgccctgtc cttgaggttg agccggatca cttcctgccc
     2701 ggcgtgcagc gccgcatacc aggccgggca tgcggcggcc agggcgtcgc cgccgggcgg
     2761 ttcgactttg cagacggcgg cgcccagcgc gtggagccgg gcgacggcca gcgggccggg
     2821 gatgttgccc gccagcgaca gcacgcgcaa gccggccagc ggccctgcct tagcgggcca
     2881 gttcacggac catgatgttg tgctggatct cgctggtgcc ctcatagatc tgcaacacct
     2941 tggcgtcgcg caacagcttc tcgaccgggt attcggtgct gtagcccatg ccgccgtgga
     3001 cctggatggc ctcggtggcc gcccacatcg ccgtgtcggc ggcgaaggct ttggcgtagg
     3061 cggcttgcag cgtgttgcgc tggccggagt cgtgcagcca ggcggcctgg taggtcagca
     3121 ggcgggcggc ttccaggcgc aggcccatct gcgcgatctt gtggccgatg gcctggtgct
     3181 ccaggatggg ccggcccatc gtctggcgcg tcttggcgta gcttagcgcc tcgtccaggc
     3241 agcgcgccgt cacgcccacg cccatggccg cgaccatggg ccgcgaacgg tcgaagacgc
     3301 ccatggcgat gcggaagccc gtgccctcct cgcccatgcg cgcgctgacc ggcacgcggg
     3361 cgttgtccag gtggatctcg gcggtgggcg cggccttctg gcccagtttg cccatcttgt
     3421 ggcctaccgt cacgcccggc gtgtcccgct cgacgatgaa ggcggagatg ccgcggtgcc
     3481 cggcggcggg gtcggtcttg gcgaacagga cgatgaagct cgcttcggtg gcgttggaga
     3541 tccagacctt ggcgccgttc agcacgtact cgtcgccgtc gcggcgagcg ctggtggtga
     3601 tggcggccac gtcggacccc gcgcccggct cggtcagggc gtagcagccg agttcgcccg
     3661 tgcgcaggcg cggcagatag gcggcctgct gctccggcgt tgccccgacc agcagcgcgt
     3721 cggccaccag gccgttgatc gtcatggccg cggtgatgcc cacgcagccc caggccagtt
     3781 gctcgaccac gatgcactgt tccaggccac ccaacccggc cccgccgtag gcctcgggca
     3841 tggtcaggtt gagcaggccc agctcctgcg cccgctcgaa cagggggcgc gggaaccgct
     3901 ctgcgcggtc atactcggcg gcgacggggc ggatctcgcg gtccgcaaac gtgtgcgcca
     3961 ggtcgcggat catgcgctgc tctgcgctta attggaagtc cacagtgcct ccctttcctc
     4021 taatcaaccg tcattctgcg cgccgcgaag aatctcgtct cctctacaat caaccgtcat
     4081 tctgcgcgcc gcaaaggatc tcgtctcctc tgttgcacgg tctatcttgg ggatggttac
     4141 ctctatgcta gcacaaatgg cgtgccggtt caatcctggc gagtcgggcg ccgccccgga
     4201 ggctcaagcc tccggc
//
LOCUS       DASUYC010000985         3077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1318899, whole genome shotgun sequence.
ACCESSION   DASUYC010000985 DASUYC010000000
VERSION     DASUYC010000985.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1318899"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            275..1177
                     /locus_tag="VF276_16140"
     CDS             275..1177
                     /locus_tag="VF276_16140"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6608432.1"
                     /translation="MKQILVPLDGSPAAEAMVPYARVWAQTIDAALLLLQVVRAPRVD
                     DPLTGIVSDTDSDPYPVWETRRAEAGAYLERIAAHLAAEQVPVETAIREGDPATVITA
                     MASGGDGVVGIAMRTHGRRGLERVIFGSVAEAVLHAVPKPILVLGHRPPPDVPTPAGP
                     YRTILVPLDGSPAAEVAIHQAQLLATPTNAALALVTVAQPPAGVPSELSWAVETVPST
                     DRGYEDYMTRVAQRVESAGLKVQTRIATGRRAATIRQCAAQAGADLIVMVTHRRAGLE
                     RFVYGSVSIQVVQGGVCPVLLIPT"
     gene            complement(1243..2211)
                     /locus_tag="VF276_16145"
     CDS             complement(1243..2211)
                     /locus_tag="VF276_16145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608433.1"
                     /translation="MPGRSLTPLEVPGAAASELLPRQDSLADELRFLLHSAVFTLSDG
                     YVPPWRYQIDGNRADLYVDFERTWQGTDPAGREMTVACGAALSYLEVVALRRGRAVAI
                     APYPDPTTPDLVARVRLEPGAAAGRPRPAGRERLVWDTAPAPPVAVVRDALEAAVAGT
                     KVWLHLEQDPDQCATVAGLIAESDAQHLRDPLRRRAETAHQGPNRWRLPPQPPAGRLE
                     PFVIHPAAGRRAAAARLEPGATGRPVFAVLGTAGDSRHEWLQAGQALGRLLMQARRDR
                     IVPAFRNQLIRIPVLRARLQELIGRDGFPQVLLALVSAARHPGGSW"
     gene            complement(2623..>3077)
                     /locus_tag="VF276_16150"
     CDS             complement(2623..>3077)
                     /locus_tag="VF276_16150"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEX6608434.1"
                     /translation="ALLAAVPEGRMTAPVLDGQWSVKDVIAHITWHEREMLGVLRARA
                     LVGSDLWDLPLHERNATIYTQHRDRPLAEVRAESRAVYPQLLAALDGLADADLTDPAR
                     FADMPGDWMPWQLLAENTYTHYRDHMPAIRAWLAQESGPGATPISGAG"
BASE COUNT          453 a         1115 c         1083 g          426 t
ORIGIN      
        1 ttctcctgcc tggttctcgg ccgctggcgc cggcctccgg gcgccgcgat tgtcatgaac
       61 tgtttaccgt ccaccacccg ttttttgacc gcaatctgat aaagtgaagg tacggatgaa
      121 tccacgccgc cgatcactcc accctcacgg cagccagggc ggcgggaacc gtgtagccgg
      181 gccatgatcg cacccgcggc gccgtccgcg ggtgcggccc ccgtctcccg gccgtgccgg
      241 tggcgggcca gggcccttga ggagggcaga cccgatgaaa cagattctgg ttccactcga
      301 cggctcgccg gcagccgaag ccatggtgcc gtatgcgcgg gtctgggccc aaaccattga
      361 cgccgccctg ctcctgctgc aggtggtccg ggcgccgcgc gtggacgatc ccctgaccgg
      421 catcgtctcg gacaccgact ccgatcccta tccggtctgg gaaacgcggc gcgccgaggc
      481 cggcgcctac ctggagcgga tagccgcgca cctcgccgcc gagcaggtgc cggtcgagac
      541 ggcgatccgc gaaggcgacc cggcgaccgt gattacggcg atggcatccg gcggggacgg
      601 cgtggtgggc atcgccatgc gcacgcacgg gcgccggggc ctggagcgtg tcatcttcgg
      661 cagcgtggcg gaagccgtgc tgcacgccgt gcccaagccg atcctggtcc tgggccaccg
      721 gcccccgccc gacgttccga cccccgcggg accctaccgc acgatcctgg tgccgctcga
      781 cggctcccct gccgctgagg tcgctatcca ccaggcgcaa ctgctggcca cgccgacgaa
      841 cgcggcgctc gccctggtca ccgttgcgca gccgcccgcc ggcgtgccct ccgaactctc
      901 gtgggcggtc gagacggtgc cgtccacaga tcggggttat gaagactaca tgacgcgggt
      961 ggcgcagcgc gtggagagcg ccggactcaa ggtccagacg cgcatcgcca ctggccgccg
     1021 ggcggcgacg atccggcaat gcgccgcgca ggccggcgct gacctgatcg tcatggttac
     1081 ccaccggcgc gcgggcctcg aacgcttcgt gtacggcagc gtctcgatcc aggtggtcca
     1141 gggcggagtc tgcccggtgt tactgatccc gacgtaagca atagcgcccc gccggtggtt
     1201 gccggcgggg cgccctcatg cgtggaccgg ctgcgctacg gcctaccagc tcccgcccgg
     1261 atgccgggca gcggatacca gcgcgagcaa cacctgcgga aaaccatcgc gcccgatgag
     1321 ttcctgcaga cgtgcgcgca gcacgggaat gcggatcaac tggttgcgga aagcgggcac
     1381 gatccggtcg cgccgtgcct gcatcaacag ccgccccagc gcttgcccgg cctgcagcca
     1441 ttcatgccgg ctatcccccg cggtgcccag cacggcgaac acggggcggc ccgtcgcgcc
     1501 cggctctagc cgcgccgccg ctgcccggcg gccggcggcc gggtggatca caaacggttc
     1561 gagccgcccc gccgggggct gcggcggtaa ccgccagcga ttcggcccct ggtgagcagt
     1621 ctccgcccgc cgccgcaacg ggtcccgcag gtgctgggca tcgctttcgg cgatcaaccc
     1681 cgccacggtg gcgcactggt ccgggtcctg ctccaggtgc agccagacct tcgtgcccgc
     1741 cactgccgct tcgagcgcgt cgcgcaccac ggcgaccggc ggcgcggggg cggtgtccca
     1801 caccaggcgt tcgcgccccg cgggccgggg ccgtccggct gccgcgcccg gttccagccg
     1861 cacccgcgcc accaggtcgg gcgtggtggg atcggggtag ggcgcgatgg cgacggcgcg
     1921 cccgcggcgc agcgccacga cctccagata gctgagcgcc gccccgcacg ccaccgtcat
     1981 ttctcgcccc gccgggtcgg tgccctgcca ggtgcgctcg aaatcgacat acaaatcggc
     2041 gcggttcccg tcgatctggt accgccacgg cggcacatag ccgtcggata gggtgaacac
     2101 ggcggaatgc aagaggaacc gcagttcatc ggccagcgag tcctggcgtg gcaatagctc
     2161 cgaggcagca gcacccggta cttccagggg agtcaacgaa cgacctggca taggagtccc
     2221 tccttgtcta gccggccggc ggcagcgggc ggggggctgt tcaacagcag gccccaactc
     2281 cgcatagcag ggcggcatga ccagcgaacg ccgggcaatc gggcggcggc cagggcgacg
     2341 acgcaggggc aggatgcggc cgtggggcag ggacgagatt ccggcaagcg tggatcggcg
     2401 ggcaggcgcg cgatccggga tcgggccggc ggtagcgggc catgcgcggg atgggggcgc
     2461 ccggcgcaga cggcgcgggc ggcgcgcatg ggcgggccgg ctcaggcgtt gcgccggtac
     2521 ggctcaggcg caacattatg caattagaaa atgagcaatc gcccgcgggg cgatagtcac
     2581 ctgctcaagt atagcacgtt ctttcccgcc aaaaccaccc tgtcaaccgg ccccgcttat
     2641 cggggtcgcg ccggggcccg attcctgggc cagccacgcc cggatcgcgg gcatgtggtc
     2701 gcgatagtgc gtgtaggtgt tctcggccag cagttgccag ggcatccagt cgcccggcat
     2761 atcggcgaag cgcgccgggt cggtcagatc ggcgtccgcc agcccgtcca gggcggccag
     2821 cagttgtggg tagacagcgc gcgactccgc ccgcacctcg gccaggggcc ggtcgcggtg
     2881 ctgcgtgtag atggtcgcgt tgcgctcgtg cagcggcaag tcccacaggt ccgacccgac
     2941 cagcgcgcgc gcccgcagca cgcccagcat ctcgcgctcg tgccaggtga tatgcgcgat
     3001 cacgtccttc accgaccact gcccgtcgag caccggcgcg gtcatgcgcc cctccggcac
     3061 ggcggccagc agcgcct
//
LOCUS       DASUYC010000986         3241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1334586, whole genome shotgun sequence.
ACCESSION   DASUYC010000986 DASUYC010000000
VERSION     DASUYC010000986.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334586"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..2995)
                     /locus_tag="VF276_16155"
     CDS             complement(104..2995)
                     /locus_tag="VF276_16155"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6608435.1"
                     /translation="MDHVSKQEALDYCLANPDRLSAAELLARFPAYREELEPLLVLGD
                     RLRHAPVPAVPPERRAAMRRRLIAAAERARTSHPRPLAADAPPALPLRNGHHLTRPVP
                     AGADAPAALIPFVGRQAELDQLRQLIDRTLRGPVHAGQVVAVTGGPGIGKSRLAGEVL
                     HEILDTLPAVQIASDRCRSDEQTLPYAGIGRLLRQVLRLAPRASRQAQAPALRRQVGD
                     LLPGWERFAPLLGALLNLPLRDTRLTAALTGAQRRDRLHELIIALCRAAAQRRPLVLV
                     LDDLQWIDPSSHALIARLAAELADSAILLLLVYRPDPGLAEPWTALGHSTTLALAPLP
                     RPASATLLGALLQGEPPAELAPLIDYGAGTPFVLEESVRYLRESGALQRDRMGDWMCI
                     RPLDRAALPREVEALISARLDRMDTATRAVAQAAAVAGQGFAAPLLARALGSAPALAA
                     RLDALVGAAVLVREGSGGPPRYQFRHTMIRDAVYDGLRPAQRRELHSQVAAAIAQAPA
                     ADLDEQQALLAQHYQQAGRPAEALPHFLAAARQAQARYAHAEARALYAQAALCARWRD
                     RALAPADLPLALPLYETWGDVLALTGDYAEARDHYSSLLAVPAGSTLPDRAEQQARLQ
                     RKIGSTYENQGQLPTALEWLNRAAAGFAAAPPSAEATLEDANVRSDLGWTYWRQGDLA
                     QAQQYLEQALARAESVGGIEQQARILNRLGGVAWARGDMPMARQYVEQSMVAYRRTGD
                     LVGQANALNNLGVLDERQGRLGDSVRHGKQAILINERLGNRRGQAIGAITAGYALYDR
                     GDYTQARYYLVQALQHAGAVHDTYHRMLALLNLGRVLTALHHWDAAAVALQESAIIAQ
                     QLQLPAEQLDSYVAQGELALQRGDLLAARQAHDQGQALARLPDTPETEELARFERFGA
                     RLALAQGQPAAARDLLDAAIARFIRLQNVPEIERTRHLRAEIVAPAAPD"
BASE COUNT          425 a         1100 c         1251 g          465 t
ORIGIN      
        1 gtgacaagga tagacggggg gcgcggagcc gggttcacaa ctgaggacgg atggccgatg
       61 ccacccgccc tcgcgttttg ctctgtgcct ggcgtgcgtt gcgtcagtcg ggcgcagcgg
      121 gagcgacgat ctcagcgcgc aggtgccgcg tgcgctcgat ttcgggcaca ttttgcagcc
      181 ggatgaaacg cgcgatggcg gcgtccagca gatcgcgggc cgcggccggt tggccctggg
      241 ccagcgccag gcgggcgccg aagcgctcga accgggccaa ctcttcggtt tccggggtgt
      301 ccggtagcct cgccagcgcc tgcccctggt catgagcctg gcgcgcggcg aggagatcgc
      361 cccgttgtaa cgccagttcg ccttgcgcca catagctgtc gagttgttcc gccggcaact
      421 gcaactgctg ggcgatgatc gcgctttcct ggagcgccac cgcggcggcg tcccagtggt
      481 gcagcgcagt gagcacgcgc cccaggttca gcagggccaa catgcggtgg taggtgtcat
      541 ggaccgcccc ggcatgttgc agcgcctgga ccagatagta gcgggcctgc gtgtagtcgc
      601 cccggtcgta gagcgcgtag ccggcggtga tggcgccaat ggcctggccc cggcggttgc
      661 ccagccgctc gttgatgagg atggcctgct tgccgtggcg gacggagtcg cccaggcggc
      721 cctggcgctc gtcgagcacg ccgaggttgt tcagcgcgtt ggcctggccc accaggtcgc
      781 cggtccgccg gtaggcgacc atgctttgct cgacgtactg gcgggccatc ggcatatcgc
      841 cgcgcgccca ggcgacgccg ccgagccggt tgagaatgcg cgcctgctgc tcgatgccgc
      901 ccacgctttc cgcacgagcc agggcctgct ccagatattg ctgcgcctgc gccagatcgc
      961 cctgccgcca gtaggtccag ccgagatcgc tgcgcacgtt cgcgtcttcg agcgtggcct
     1021 cggcggaggg cggcgcggcg gcaaagccgg cggcggcgcg attcagccac tccagcgcgg
     1081 tgggcaactg gccctggttc tcataggtgc tgccgatctt gcgttgcaag cgcgcttgct
     1141 gctcggcgcg atcgggcagc gtgctcccgg ccggcaccgc cagcaggctg ctgtaatggt
     1201 cgcgcgcctc ggcataatcg ccggtcagcg ccagcacgtc gccccaggtc tcgtagagcg
     1261 gcagcgccag gggcaggtcg gcgggggcca gggcgcggtc gcgccagcgg gcgcagagag
     1321 ccgcctgggc gtagagcgcg cgggcctcgg cgtgggcgta gcgggcctgg gcctggcggg
     1381 cggcggccag gaaatgcggc agcgcctcgg cgggccgccc ggcctgctgg tagtgctgcg
     1441 ccagcagcgc ctgctgctcg tcgagatcgg cggcgggggc ctgcgcgatg gccgccgcga
     1501 cctggctgtg gagctcgcgc cgctgggcgg gccgcaggcc gtcgtagacc gcatcgcgga
     1561 tcatggtgtg ccggaactgg taccggggcg ggccgcccga tccctcgcgc accagcaccg
     1621 ccgcgccgac cagggcatcc agccgcgcgg cgagcgccgg cgcactgccc agggcgcgcg
     1681 ccagcagcgg ggccgcaaag ccctgcccgg ccaccgccgc cgcctgggcc acggcgcggg
     1741 tggcggtgtc catgcggtcg agccgcgcgc tgatcagcgc ttcgacctcg cggggtaggg
     1801 cggcgcggtc gagtgggcgg atgcacatcc agtcgcccat gcggtcgcgc tgcagagcgc
     1861 ctgattcgcg cagatagcgc accgattctt cgaggacgaa gggggtgcct gcgccgtagt
     1921 cgatcagcgg ggccagttcg gcgggcggtt cgccctgcaa cagggcgccc agcagggtcg
     1981 cgctggcggg ccggggcagc ggggccaggg ccagcgtcgt gctgtgcccg agcgcggtcc
     2041 acggttcggc gagtccgggg tcgggccggt atacgagcag cagcaggatc gcgctgtccg
     2101 ccaactccgc cgccagccgg gcgatcaggg cgtggctcga cgggtcgatc cattgcagat
     2161 cgtcgaggac gagcacgagc ggccggcgct gggccgcggc ccggcagagg gcgatgatca
     2221 gctcgtgcag ccggtcgcgc cgctgcgcgc cggtgagcgc ggcggtgagg cgggtatcac
     2281 gcagcggcag gttgagcaac gcgcccagca gcggggcgaa gcgttcccag ccgggcaaca
     2341 ggtcgcccac ctgccggcgc agcgccggcg cctgggcctg gcgcgaggcc cgcggcgcga
     2401 ggcgcagcac ctggcgcagc aagcgcccga tgcctgcgta gggcagggtc tgttcgtcgc
     2461 tacggcagcg gtcgctcgcg atctggacgg cgggcagcgt gtctaggatc tcgtgcagca
     2521 cttcgccggc cagccgcgac ttgccgatgc ccggcccgcc ggtaacggcc accacctggc
     2581 ctgcatgcac cgggccgcgc aacgtccggt cgatcaattg gcggagctgg tcaagctcgg
     2641 cctggcggcc cacgaagggg atgagcgcgg cgggtgcgtc cgcgccggcg ggaacggggc
     2701 gggtgagatg gtgcccgttc cgcagcggca gcgccggcgg cgcgtccgct gcaagggggc
     2761 ggggatgcga ggtgcgggcg cgctcagcgg cggcaatcag gcggcggcgc atggcggcgc
     2821 gccgctccgg cggcacggcg ggcacggggg cgtggcgcag ccggtcgccc aggaccagca
     2881 gcggttccag ttcttcccgg taggcgggga acctggcgag taattcggcg gcgctcaacc
     2941 tgtcgggatt agccagacag taatcgagcg cctcttgctt gctgacgtga tccacgtttc
     3001 cctcaatcgc cccaaaccgc gcccactggg atgacgctgg ccgccggtgc agagttcccg
     3061 gcggcgcccc gcgccgtttg cgccctcgca cctatattat atagtatgtt gggaaagaat
     3121 ggatcgcgat ccggaataca attgtccatt tgcccgccag cgcgacaggt gcgctgcatg
     3181 tcgcgggcgc cgggcggtga tctgccgagt gcgccctgcc gcgcggggcc gcaggtgcta
     3241 t
//
LOCUS       DASUYC010000987         2386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1599592, whole genome shotgun sequence.
ACCESSION   DASUYC010000987 DASUYC010000000
VERSION     DASUYC010000987.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1599592"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2223
                     /locus_tag="VF276_16160"
     CDS             <1..2223
                     /locus_tag="VF276_16160"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6608436.1"
                     /translation="QNRIPTPPAVPDVQRSIHSGAASSLPAKLGDNPIPAAAPRPVTD
                     RGALPLVARAPEWQTLLETYNTITEHGRMVVLQGEAGIGKTRLAEEFLAHARAAGAIT
                     LAARAYEGEQTLAYGPLVAALRLALDGPDNAGPRDPIPDVWRREAAQLVPDLAGPGTP
                     PEPPRAYDQAAQARFFEGIRQVLLKRTNGSAPGVLFFDDLQWADGATVEFLTYFARRL
                     QRAPVCLLLAWRTEQQPEGRQLDPILAGASRAVEGVILPLGRLRPDAVAELVRWAAVV
                     PPAETPALVARLYAETEGLPFFLREYLAALAGGSYAPGVDPWPLLGGVRDLLHSRLRT
                     ISETGLQVLTAAAVIGRSFDFDTVRAASGRTEDEAVAALEELITAGLIVEVRGSLAPG
                     EAPMYDFTHDKLRVLVYAETSLGRRRLLHHRVAEALQNPARNARHAQPLAGRIAYHYH
                     HAGNEERAAYYYKLAGDRARSLYANREALAHLQTALALGHPESVELHIALGDLHTLLG
                     AYRAAINSYETAAGLAVDRRVAEIEHRLGLVYQRRGQWELATAHLATALDAYTRAGEP
                     AARARVYTDWSLTALQQGAAEQAHELARQALALAGNSEDSRARARAHNALGLIASKRG
                     DPMSARTHLEQSLALADALGDIEVRAAVLNNLALAHGVAGEIDRALDLAAAALDLCSV
                     RGDRHREAAIHNNIADLLHAAGRADEAHRHVEQAVAILGEIGAEAGDLQPAIWMLSAW
                     "
     gene            complement(2210..>2386)
                     /locus_tag="VF276_16165"
     CDS             complement(2210..>2386)
                     /locus_tag="VF276_16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6608437.1"
                     /translation="LRQLFTRPAPRRNPYSTPNPRIYICSSSTPPGGGVHGMCGYHAA
                     QAVLRAVPGPATTR"
BASE COUNT          378 a          843 c          830 g          335 t
ORIGIN      
        1 caaaaccgca tcccgactcc accggcggtg ccggatgtcc agcgatccat ccattcaggc
       61 gccgcatcga gcctcccggc gaagctgggg gacaacccca tccccgccgc ggcgccccgt
      121 cctgtcacgg accgcggcgc gctgcccctg gtggcacggg ccccggaatg gcagacgctg
      181 ctggagacgt ataacaccat caccgagcat gggcgcatgg tggtgctcca gggagaggcg
      241 gggatcggca agacgcggct ggccgaggag tttctggccc acgcgcgcgc ggccggcgcc
      301 atcacactcg ccgcgcgcgc atacgaaggc gaacagaccc tggcttacgg gccgctggtc
      361 gccgccttgc ggctagcgct cgatgggccg gataatgctg ggccgcgaga tcccataccg
      421 gacgtctggc ggcgcgaggc ggcgcaactg gtgcccgatc tcgccgggcc cggcaccccg
      481 ccggagccgc cacgggcata tgatcaggca gcgcaggcgc gctttttcga gggaatccgc
      541 caggtgttgc tgaagcgcac caacggatca gcacccggcg tgctcttctt tgacgatctg
      601 cagtgggccg acggcgccac ggtcgagttc ctgacgtatt tcgcccggcg cctgcagcgg
      661 gccccggtgt gtctgctgct cgcctggcgc acggagcaac aacccgaggg ccggcagttg
      721 gacccgatcc tggccggcgc gagccgtgct gtcgaaggcg tgatcctacc gctgggccgc
      781 ctccgcccgg acgccgtggc cgaactggtc cgctgggccg ccgtggtccc accggccgaa
      841 acgccggcgc tggtcgcccg gctctacgcg gaaaccgagg ggctgccctt cttcctgcgc
      901 gaatacctcg ccgcgctggc cggcggtagc tatgcgcccg gcgtagaccc gtggccctta
      961 ctggggggag tacgcgatct gctgcactca cgactgcgca cgatcagcga gaccggatta
     1021 caggtgctca ccgcagccgc ggtgatcggc cggtcatttg atttcgacac ggtccgcgcg
     1081 gccagtgggc gcacggagga cgaggccgtc gccgcgctgg aagagttaat caccgccggg
     1141 ctgatcgtgg aggttcgcgg tagcctggcc ccaggcgaag cgccgatgta tgatttcacg
     1201 cacgacaagc tccgcgtgct ggtttacgcg gagaccagcc tggggcgccg ccgcctgctg
     1261 catcaccggg tcgcggaagc cttgcagaac cccgcgcgca acgcgcggca tgcgcagcca
     1321 ctggccggcc ggatcgccta tcattaccat cacgcgggca acgaggagcg cgccgcctac
     1381 tattataaat tggctggcga ccgtgcccgc agcctctacg ccaatcgcga agccctggcc
     1441 catctgcaga cggcgctcgc cctgggacat cccgaaagtg tcgagttgca cattgctctc
     1501 ggcgatctgc acacgctgtt aggcgcctac cgcgcggcga tcaacagcta tgagaccgcc
     1561 gccggcctcg cggtggaccg gcgggttgcc gaaatcgagc accggctggg tctcgtgtac
     1621 cagcggcgcg gccagtggga gttggctacc gcgcacctcg ccacggcgct ggatgcctac
     1681 actcgggcgg gagagccggc cgcccgcgcg cgtgtttata ccgattggag cctgacggcg
     1741 ctccagcagg gggcggcaga gcaggcccac gaactggccc ggcaagccct tgcgctggcc
     1801 ggcaacagcg aggacagccg ggcgcgcgcc cgcgcccaca acgccctcgg cctcatcgcc
     1861 agcaaacgcg gcgaccccat gagcgcacgc acccacctgg agcagagcct agcccttgcg
     1921 gacgcgctcg gcgacatcga ggttcgcgcc gccgtgttaa acaacctggc gctggcgcac
     1981 ggcgtcgcgg gggagatcga ccgggcactc gacctggcgg cggccgccct ggatctttgc
     2041 agcgtgcgcg gcgaccggca ccgcgaggcg gcgatccata acaacatcgc ggacctgttg
     2101 cacgcggccg gccgggcgga cgaggcgcac cgccatgtcg agcaggccgt cgccatcctg
     2161 ggcgagatcg gtgccgaggc gggggacctg caacccgcca tctggatgct cagcgcgtgg
     2221 tagctggtcc cggaaccgcc cgtaacacgg cctgcgcggc atggtagccg cacatgccat
     2281 gcacgccccc gccgggcggc gtggacgacg agcaaatgta gatccgcgga ttgggcgtgc
     2341 tatacgggtt ccggcgcggc gcggggcggg tgaacaactg gcgcag
//
LOCUS       DASUYC010000988         3260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_152184,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000988 DASUYC010000000
VERSION     DASUYC010000988.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152184"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VF276_16170"
     CDS             <1..429
                     /locus_tag="VF276_16170"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF039343.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HEX6608438.1"
                     /translation="FELQVQGYLPILAHPERYVAIQHDPNVLYRLAERGILAQVTAGA
                     FAGYFGEATRRCAVTLTEHGLVQFLASDAHSSIDTRRLPALDQGYRDLVGLVGEPTAT
                     VMATTAPSQVPADAPVAIDARPVEAPRSFFSRMFGGQQGH"
     gene            429..626
                     /locus_tag="VF276_16175"
     CDS             429..626
                     /locus_tag="VF276_16175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608439.1"
                     /translation="MAGPAAAWIAARALLAEATPTPDPSGLTMRPPSSGEAWIMLLMI
                     AGIVAVFALAWWNSRRDERKP"
     gene            complement(853..>3260)
                     /locus_tag="VF276_16180"
     CDS             complement(853..>3260)
                     /locus_tag="VF276_16180"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX6608440.1"
                     /translation="VGLALVALVFLAVVALVAGRGLGAARQGGAAGGSGAGPANVDFF
                     PAGGSDRPVATPFVSDTFVDPWPDDPALAAAAQAEYEGRYEDAAALYRPVAEARSAQP
                     ASADARWHLAVSLFDAGQYDAAIDNFAVYARTYPTDTRSLLAAFWAGRAHRAAGRPAE
                     AEAEFRSYAAQGGPLRATALLLAADAATAQGHQDAVRELINRVLAEPTTRLDRMAAWE
                     RLAALETANQAPDKAAAWYAAIAQEAHMPGYRAQMRLKAGIALLAAHEQAKGEAALRD
                     LIQAQPDDPAAYQALHRLLDSDPQVLSAGRLPYDLACRVAYAAGKYSEAIDYCESFRT
                     VQAPGPDRADAAWYTARAYQAAGNTTLADSWYKGFTEVYTADARLPDVLYQWAGLRAG
                     AGDVTGALTLYDYLAQAYPQKAVGADAQDQAAVLLRKQGDLAGAAEHWRIAAGLPGAD
                     DVTRARALFWQGWALQQQGQADAAAALWRQGAAYHTFWGQRCLDHLAAETVVPRDNGP
                     TAAAQDRLRALAAPDPQQETTAARDLLEWAVQWSRPGSVAPAPGELPARLRDDPAYGR
                     AVGLAQVGMWTEARLAFDDLASTLAEENDGVALAALALQAQRARWPWIALLVAGDLQG
                     AAAAAHAPAGIADLPRAAQEMLYPVGWPQLVAAQAQDQGVDPWLLLGLVRQESAFDPA
                     AHSSADARGLTQVIPGTASGIAAALHLPDFQQAHLYRPAISLRFGAYYLQSTLTRFDR
                     NILYALAGYNGGPANVPDWASGRVNDDPDLFVDNIDFPETRQYVQIVYNNYAIYRWLY
                     GER"
BASE COUNT          451 a         1190 c         1145 g          474 t
ORIGIN      
        1 ttcgagttgc aggtacaggg ctatctgccc atcctggccc accccgaacg ctacgttgcc
       61 atccagcacg acccgaacgt gctctatcgg ctggccgagc gcggtatcct ggcccaggtg
      121 acggcgggcg ccttcgcggg ctacttcggc gaggccaccc ggcgctgcgc cgtgacgctc
      181 acagagcatg gcctggtgca gttcctggca tccgacgccc acagcagcat cgacacccgc
      241 cggttgccgg ccctggacca gggctaccgc gatctagtgg gcctcgtcgg cgagccgacc
      301 gcgacagtga tggcgaccac cgcgccctcg caggtgccgg ctgatgcccc ggtggcgatt
      361 gacgcccggc ccgtcgaagc cccgcgctcc ttcttctccc gcatgttcgg cgggcagcag
      421 ggccactgat ggccggcccg gcggccgcct ggatcgcggc ccgtgccctg ctcgccgagg
      481 ccacccccac gcccgaccct tccggcctga ccatgcgccc acccagcagc ggcgaggcct
      541 ggatcatgct gctgatgatc gcaggcattg tggccgtctt cgccctcgcc tggtggaaca
      601 gccggcgcga cgaacgcaag ccctagctat cttacatcct ccgtacccag tagcctgtca
      661 cgcccccttg gtcattctga gcgcagcgaa gaatctccgt ctcgtggggg gcaaaagacg
      721 agactcttcg ctccgctcag agtgacaagc caaaccccgt cattctaagc gcagtgaaga
      781 atctcctcgc atcgccccaa cgccgagact cttcgctgcg ctcagagtga caaccggagg
      841 gtgatcgggt gatcagcgtt ccccgtatag ccagcggtag atcgcgtagt tgttgtagac
      901 gatctgtacg tattggcggg tttcggggaa gtcgatgttg tcgacaaaga gatcggggtc
      961 gtcattgacg cgcccgctcg cccaatcggg cacgttggcc gggccgccgt tgtagcccgc
     1021 gagcgcgtag aggatgttgc ggtcgaagcg ggtgagcgtg ctctggaggt agtaagcccc
     1081 gaagcgcagg ctgatcgccg ggcggtagag atgcgcctgt tggaagtcgg gcaggtgcag
     1141 cgcggcggcg atgccggacg ccgtgccggg gatcacctgg gtcaggccgc gcgcatcggc
     1201 gctggagtga gccgccgggt cgaaggcgct ctcctggcgc accaacccca gcaacagcca
     1261 tggatcgacg ccttggtcct gggcctgcgc ggcgacaagt tgcggccagc ctaccgggta
     1321 cagcatctcc tgcgcggccc gcggcagatc ggcgattccg gcgggggcat gggcggcagc
     1381 ggcggcccct tgcaggtcgc cggccaccag gagggcgatc cagggccagc gcgcccgctg
     1441 cgcttgcagc gccagcgcgg ccagcgccac gccgtcgttc tcctcggcca gcgtcgaggc
     1501 caggtcgtcg aacgccagcc gcgcctcggt ccacattccc acctgcgcca gccccaccgc
     1561 gcggccatag gccgggtcgt cgcgcaggcg cgcgggcagt tcgcccggcg cgggcgccac
     1621 gctgcccggc cggctccact gcaccgccca ttccaacagg tcccgcgccg ccgtggtctc
     1681 ttgctgcgga tcaggggcgg cgagggcgcg caggcgatcc tgggccgcgg cggtggggcc
     1741 gttgtcgcgc ggcacgaccg tctccgcagc caggtggtcg aggcagcgct ggccccagaa
     1801 ggtgtggtag gccgcgccct ggcgccagag cgccgccgcc gcatcggcct ggccttgttg
     1861 ctgcaacgcc cagccctgcc agaagagcgc gcgtgctcgc gtcacgtcgt cggcgccggg
     1921 cagcccggcg gctatccgcc agtgctctgc cgccccggcc aggtcgccct gcttgcgcaa
     1981 cagcaccgcg gcctggtcct gggcatcggc gccgaccgct ttttgcgggt acgcctgggc
     2041 caggtaatcg tagagggtca gcgcgcccgt cacgtccccg gcgccggccc gcagccccgc
     2101 ccactggtag agcacatcgg gcaggcgggc gtcggcggta tagacttcgg tgaagccctt
     2161 gtaccaggag tcggccaggg tggtgttgcc cgcggcctgg taggcgcgcg ccgtgtacca
     2221 ggcggcgtcg gcgcgatcgg ggccgggcgc ctgcaccgtg cggaagctct cgcagtagtc
     2281 aatggcctcg ctatacttgc cggcggcata ggccacccgg caggccaggt cgtagggcag
     2341 ccgcccggcg ctcagcacct gcggatcgct gtccagcagc cggtgcagcg cctggtacgc
     2401 cgccggatcg tcgggctggg cctggatcag gtcgcgcagc gcggcctcgc ccttggcctg
     2461 ctcgtgggcg gcgagcagcg cgatgccggc cttcaggcgc atctgggcgc ggtagcccgg
     2521 catgtgcgcc tcctgggcga tggcggcata ccaggccgcc gccttgtcgg gcgcctgatt
     2581 cgccgtttcc agggcggcca gccgctccca ggcggccatg cggtccaggc gcgtcgtcgg
     2641 ctcggccagc acgcggttga tcagttcgcg cacggcgtcc tgatggccct gcgccgtggc
     2701 ggcgtcggcg gccagcagca gcgcggtggc ccgcaacggc ccgccctggg ccgcgtagga
     2761 ccggaactcg gcctccgctt ccgccgggcg tccggcggcc cgatgcgccc gccccgccca
     2821 gaacgccgcc agcaaactgc gggtatcggt gggataggtc cgggcataga cggcaaaatt
     2881 gtcgatggcg gcatcgtatt gcccggcgtc gaacagggac acagccaggt gccagcgcgc
     2941 atcggcactc gcgggttgcg cggaccgggc ctcggcgacg ggccggtaga gcgccgcagc
     3001 gtcttcgtag cgcccctcgt attcggcctg cgccgccgcg gccagcgccg gatcatccgg
     3061 ccacgggtcg acaaatgtgt cgctcacgaa cggcgtagcc accgggcgat ccgatccgcc
     3121 ggcggggaag aagtccacat tggccggtcc ggcgccggac ccgcccgctg ccccgccctg
     3181 ccgggccgcg cccagcccgc gcccggccac cagggcaacc acggccagga acaccagcgc
     3241 aacgagcgcc agccccaccg
//
LOCUS       DASUYC010000989         2520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_859774,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000989 DASUYC010000000
VERSION     DASUYC010000989.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859774"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..643)
                     /gene="folK"
                     /locus_tag="VF276_16185"
     CDS             complement(143..643)
                     /gene="folK"
                     /locus_tag="VF276_16185"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX6608441.1"
                     /translation="MALVYLSLGSNVGDRAAVLRRAIAGLPAATRGRVLRVSRLYETA
                     PWGVRNQPSFFNLALALDTPLAPRDLLTALKEMEAAAGRTAGPRWGPRPLDIDILLYD
                     QLVQNDPALIIPHPRLRERRFVLQPLADIWPAGTTLLGQPLPALLRAVADQEVTPVSA
                     DQEGGL"
     gene            complement(650..1840)
                     /gene="folP"
                     /locus_tag="VF276_16190"
     CDS             complement(650..1840)
                     /gene="folP"
                     /locus_tag="VF276_16190"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX6608442.1"
                     /translation="MSPAHARLLTSTPEVLAAELAGFGAASGDTPARARASSFAAVRI
                     EGLSSAALAALAATVHAEGGELVTAEDRGEALVLGTRAQFAALGQALRAAGGVEAAQA
                     LDDLFAALDRPTPPLQLGRYPLPLGRRTLVMGILNMTPDSFSGDGLDFGVDAAVVRAQ
                     AMQAAGADILDVGGMSTRPGAEEIAESEELRRVVPLIERLAREVDVPLSVDTYRAAVA
                     DAALAAGAVIVNDITGLGAEPELAGVVTRRGAALVLMHIKGTPRTMQDDPQYADLMGE
                     IIAYLRAGCDRAVAAGVPRDHLWIDPGIGFGKTIDHNLEILRRLGELRSLGLPVLVGT
                     SRKGFLGRILGGRPPQERVAATGATVAVSIAHGAAIVRVHDVGPAVDVARVADAILRG
                     RDRS"
     gene            complement(1837..2409)
                     /gene="folE"
                     /locus_tag="VF276_16195"
     CDS             complement(1837..2409)
                     /gene="folE"
                     /locus_tag="VF276_16195"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX6608443.1"
                     /translation="MDREKIQTAVRMILEAVGEDPDREGLQGTPRRIADMYEEILAGI
                     GRDPRPFLAVTFQEQHKEMVVLRDIPLYSLCEHHMLPFHGMAHIGYIPRGRIVGLSKL
                     ARLTDLLSKRFQVQERLTSEIADAIVDGLGPVGCGVVIEAEHLCMAMRGIQRPGARMV
                     TSAMRGSFRENAATRAEFMAIIRHRPGGDG"
BASE COUNT          362 a          873 c          934 g          351 t
ORIGIN      
        1 gcgggaaggg gggaatcggc ggcgcgaggg aaagtgacgc ccctctccgc ggcggggagg
       61 ggctggggga gaggttggtc acctgcctgg atgctaaatg gtggcgcgca ctcgctgccg
      121 gcggatgccg cagaagatgg ggttacagcc cgccttcctg gtcggcggag acgggcgtca
      181 cttcctggtc ggccacggcg cgcagcaggg cgggcagcgg ctggccgagc agcgtcgtgc
      241 ccgcgggcca gatgtcggcc aggggttgca gcacgaagcg gcgctcgcgc aggcgcgggt
      301 ggggaatgat cagcgccggg tcgttctgga cgagctgatc gtagagcagg atgtcgatat
      361 cgagcgggcg cggcccccag cgtggcccgg cggtgcgccc ggcggcggct tccatctcct
      421 tcagcgccgt cagcagatca cgcggggcca gcggggtgtc gagcgcgagg gccagattga
      481 agaaggacgg ctggttgcgc acgccccagg gcgctgtttc gtagagccgc gacacccgca
      541 gcacgcgccc gcgcgtggcg gcgggcagcc cggcgatggc ccggcgcagc accgcggcgc
      601 ggtcgcccac gttcgagcct aggctgagat agaccagggc catggcgtct cagctccggt
      661 cccgaccgcg caggatcgcg tcggccaccc gcgccacatc gacggcgggg cccacgtcgt
      721 gcacgcgcac aatggcggcg ccatgcgcga tgctgaccgc gaccgtcgcc ccggtggccg
      781 ccacgcgctc ctgcggtggg cgcccgccga gaatccgccc caaaaacccc ttgcgcgagg
      841 tgccgaccag caccggcagg cccagcgagc gcagttcgcc cagccgccgc aagatctcca
      901 gattgtggtc gatggtcttg ccgaagccga tgcccggatc gatccagagg tggtcgcgcg
      961 gcaccccggc ggccacggcg cggtcgcacc cggcgcgcag ataggcgatg atctcgccca
     1021 tcaggtcggc gtactgcgga tcgtcttgca tggtgcgcgg ggtgccttta atgtgcatca
     1081 agaccagcgc cgcgccccgg cgggtcacca cgcccgccag ttccggctcg gcaccgaggc
     1141 cggtgatgtc gttcacgatc accgcgcccg cggccagggc ggcatcggcc acggcggccc
     1201 ggtaggtgtc caccgacagc ggcacatcga cctcgcgggc caggcgctcg atcagcggga
     1261 ccacgcggcg caattcctcg ctctcggcga tctcctcggc gccgggccgc gtgctcatgc
     1321 cgcccacgtc gagaatgtcg gcgcccgccg cctgcatcgc ctgggcgcgg accacagcgg
     1381 cgtccacgcc gaaatcgagc ccgtcgccgg agaaactgtc gggcgtcatg ttgaggatgc
     1441 ccatgaccag cgtgcgccgg cccaggggca gggggtagcg gcccaactgt aacggcggcg
     1501 tgggccggtc gagggccgcg aacaggtcgt cgagggcctg ggccgcttcg acgccgcccg
     1561 cggcgcgtaa cgcctggccc agcgcggcga actgggcgcg tgtgccgagc accagggcct
     1621 ctccgcggtc ctccgccgtc accagctccc cgccctcggc gtgcacagtc gcagccaggg
     1681 ccgccagcgc agcggatgag aggccttcaa tacgcacggc agcaaagcta ctcgcgcggg
     1741 cgcgcgccgg ggtgtcgccg gatgccgcgc cgaagccggc gagttcggcg gccaggactt
     1801 cgggggtgga ggtcagcagc cgggcatggg cgggactcac ccgtcgcctc cgggccggtg
     1861 gcggatgatc gccatgaact cggcgcgggt cgcggcgttc tcgcggaagc tgccgcgcat
     1921 ggccgatgtc accatgcgcg cgccggggcg ctggatgccg cgcatcgcca tgcagaggtg
     1981 ttcggcttcg atcaccacgc cgcagccgac cggccccagc ccgtccacga tggcgtcggc
     2041 gatctcgctg gtcagccgct cctgcacctg gaagcgtttg gacagcaggt cggtgagccg
     2101 ggcgagtttg ctcaggccga cgatgcgccc gcgcgggata tagccgatat gggccatgcc
     2161 gtggaacggc agcatatggt gttcgcacag cgaatagagc gggatgtcgc gcagcaccac
     2221 catctctttg tgctgctcct ggaacgtgac agccaggaag gggcgcgggt cgcggccaat
     2281 gccggccagg atctcctcgt acatatcggc gatgcgccgg ggggtgccct gcaacccttc
     2341 gcggtcgggg tcttcgccca ccgcttccag gatcatgcgc acggcggtct ggatcttttc
     2401 gcggtccatg ggttcctttc gggccggagg tgcggctagt gcctgccccg ccgccgggtg
     2461 cgccgcgcgc tcgccacacg atagatgctc tcgaacatgc gggcaatgtg cggccagttc
//
LOCUS       DASUYC010000990         2685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_627108,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000990 DASUYC010000000
VERSION     DASUYC010000990.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627108"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="VF276_16200"
     CDS             <1..907
                     /locus_tag="VF276_16200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608444.1"
                     /translation="FAVAKLVLLAVLGLGLVRQGGLLIYATWVIGSFTSLLFLAGWAW
                     RRGTPLLGHRPAWGLLRGLGRSALGHHALNLTLQIPSLALPLVVTVELSTTATAHFYV
                     AWMIAGLLFVAPYALTTVLYAVGAADPTALAHKLRQSLFLSFALCGAANLALWVAADW
                     VLAIFGQGYALEAGISLRLLALGVIPLVVRYHYIALARLRGRMHQAALLQACASGLEL
                     VLAATGAALGGLAGLSIGWLLAVTIEAMVMASAVYRAGWRGPVAPPPSIGHAIGDSPD
                     PTLAGAVWLSSELESADVAPSSKGA"
     gene            879..2132
                     /locus_tag="VF276_16205"
     CDS             879..2132
                     /locus_tag="VF276_16205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX6608445.1"
                     /translation="MSPRLQREPEHARGAPGAGRKQQGPPSRQGVTTAGDERAAAPLV
                     SIIIDNYNYGRFLATAIESALQQTYTPCEVLVVDDGSTDDSRAVIAAAGTRVTAIFKP
                     NGGQASAFNAGFTQSHGAIVLFLDADDILLPETVTHVVAAFHAHPSLVRVQYRMAVVD
                     RHGRPTGRTKPSWDQPLPDGDLGPYVLSAPFDLTWMATSGNAFATPALRRILPIPEAN
                     FVILADFYLVHLTSLLGPVKSLDEIGACYRVHGANQYEPATVGLDLAHLRRTIIYAAP
                     THTAIKALADELHLAGRPRRAGELAAVSLLAARLISLKLDPAQHPFPRDRVGRIVWRG
                     IRASARRRDVHLPLKAVFILWFLVTGPAPRPLARLLGAKFMYPETRPGWHRILAGLHR
                     RRGNHDSTTSTPRTTHALPVENEAG"
BASE COUNT          419 a          930 c          839 g          497 t
ORIGIN      
        1 cttcgcagtc gccaagctgg tactgctggc cgtcctgggc ctcgggctgg ttcgacaggg
       61 tggcctgctc atttacgcca cgtgggtcat aggtagcttc acgtcactac tctttctcgc
      121 gggctgggcg tggcgtaggg gtactccgct cctgggccac cgaccggcct ggggactgct
      181 ccgcggtctc ggccggtccg ccctgggcca tcatgcgctg aatctcacgc tgcagattcc
      241 ttcgctggcg ctaccgctcg tcgtcacggt cgagctgtcg accacggcaa cggcccactt
      301 ttatgtagcg tggatgatcg cggggctact gttcgtggcg ccctacgccc tgaccacggt
      361 tctatacgcg gtcggcgccg ccgatccgac ggcactcgcc cacaaactac ggcaaagcct
      421 gttcctgtcc ttcgccttgt gcggggcggc caacctggcg ttgtgggtgg cggccgactg
      481 ggtgttagcc atcttcggcc aaggttacgc gctggaggcg gggataagcc tgcgcttact
      541 ggccctcggg gtgattccgc tggttgtgcg ctatcactac atcgccctgg cccgtctgcg
      601 gggccgtatg catcaggcag cgctgttgca ggcgtgcgcc agcggactag aattggtgct
      661 cgcggcaacc ggcgccgccc tgggggggct cgcgggtctc agcatcggct ggctcctagc
      721 ggtaacgatc gaagcaatgg tcatggcgtc tgccgtatac cgcgccgggt ggcggggacc
      781 cgtcgccccg cctccatcta tcgggcatgc gattggggat tcgccggatc cgacgttagc
      841 cggcgccgtc tggttaagca gcgagttgga gagcgccgat gtcgccccgt cttcaaaggg
      901 agcctgagca tgctcgcggg gcgccgggcg ccgggcggaa acaacaaggc ccgccgtccc
      961 gacagggagt aaccaccgca ggtgacgaaa gggcggcggc cccgctcgtc agcatcatca
     1021 tcgacaatta taactacggc cgattcctgg caacggcgat tgaaagtgcg ctccagcaga
     1081 cgtacacgcc ctgcgaagtg ctcgtagtgg acgacggatc gacggacgat tcacgcgctg
     1141 tcatcgccgc cgctgggacg cgcgtgaccg ctatctttaa gcccaacggc gggcaggcct
     1201 cagccttcaa cgccgggttc acgcagagcc acggcgccat cgtgctgttt ctcgacgccg
     1261 acgacatact gctccccgag acggtaaccc atgtcgtggc cgcctttcac gctcatccca
     1321 gcctcgttcg cgtgcagtac cggatggccg tggtggaccg ccacgggcgc ccgaccggcc
     1381 ggacgaagcc ttcctgggac cagccgctgc ccgacggcga tctaggcccc tacgtcctct
     1441 cggcgccctt cgacctgacg tggatggcga ccagcggtaa cgcctttgcc accccggcct
     1501 tgcgccggat cttgccgatc ccggaggcga acttcgtgat cctggctgat ttctacctgg
     1561 tgcatctcac gtcgctgttg ggtccggtta agtcgctgga cgagataggg gcgtgctatc
     1621 gcgtccatgg ggccaaccaa tatgagccgg cgacggtcgg gctcgacctg gcccacctgc
     1681 gccggacgat catctacgcc gcgccgaccc acaccgccat taaggccctg gccgacgaac
     1741 tccacctggc cggccggccc cgccgtgccg gcgaactggc cgcggtctca ctgctggccg
     1801 cccggctgat ctcgctgaaa ctcgatcccg cccagcaccc cttcccgcgc gacagggtcg
     1861 gccggattgt atggaggggg atccgggcca gcgcgcgccg ccgggatgta cacctgccgc
     1921 tgaaagccgt atttatcctc tggttcctgg ttacgggacc ggctccccgc cccctggccc
     1981 gcctcctcgg cgcgaagttc atgtaccccg aaacgcgtcc gggatggcac cgcatcctcg
     2041 ctggactcca ccggcggcgg ggcaatcatg attccaccac tagcaccccg cgcaccaccc
     2101 acgccctgcc agtagaaaac gaggccggtt agcgccgcca ggccgaaccc cgtggcaatt
     2161 accctgccga cccccatcgc cctaaacgcg caatgtcaca atcccatcac atcaatcgag
     2221 attccattgc ttcggacttt cgggatcgag tagaataatc ccgaccaggg acccgcagta
     2281 ggacatggtc tcgcaaatgc aatggaagga tcacttgcct ggtttgctct ggaattgagt
     2341 ggggtgacgc gccggtggcg cggtctcggt tcattcaccc ccttggccac tcgcccgcgt
     2401 agtcgcgcct tgcctcgcgc cctctcttct tatcccctat gcgccgggcg acctatgacc
     2461 gctcgtaccc tgacctccgt tggcctggac cgggtatctg agttaccgac cccactagag
     2521 ttgggggacg gcattccgca ctgtacctgg ccgcaggtgc cgggaatcag ggtggcggtg
     2581 gaaccggtga cggaccgctt gggcggttag cgccggctcc tccacgcgga cgcgctccta
     2641 tggcgatctg ctccccgctg gccaaccggt ctgccgaaac ggaga
//
LOCUS       DASUYC010000991         2861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1359690, whole genome shotgun sequence.
ACCESSION   DASUYC010000991 DASUYC010000000
VERSION     DASUYC010000991.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359690"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..333)
                     /locus_tag="VF276_16210"
     CDS             complement(109..333)
                     /locus_tag="VF276_16210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608446.1"
                     /translation="MSTTPPDDDYIPGYATRKEASPAPGSAGFDDTPETRTPWNARSG
                     ALIMVGIVLACLAITLAVILFGGLGAVVWR"
     gene            complement(350..1207)
                     /locus_tag="VF276_16215"
     CDS             complement(350..1207)
                     /locus_tag="VF276_16215"
                     /inference="COORDINATES: protein motif:HMM:NF021384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HEX6608447.1"
                     /translation="MMRVLKERSLPISPLQAGLIAVLLGLLVFVPGSLKYKLDFLGFG
                     VCHQLSSHSLFIAGHQLPLCARCSGIYLGALVTFIMLLILRPLAGGLPSRRIFPLLGF
                     MFSTMVFDGINSTFDSLPGATALYETTNWLRLITGVLAGVSLMFVLYPLFNQSFWRTG
                     LLHRERSIDQPFELFGYLVPTAVVVGIMLSAAGDTRIGDWLYWPLALGSVGGLLALLT
                     MTNVLIVSTMSRRAGSVLTWQMALTPLLIALILALIELTAMANLRIALTGVLATSATP
                     DLPLQPGLR"
     gene            complement(1204..2700)
                     /locus_tag="VF276_16220"
     CDS             complement(1204..2700)
                     /locus_tag="VF276_16220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608448.1"
                     /translation="MHRTTPRRGARWLLAVLLGTAAGTGVLAARPAAAVELRYADPAF
                     QQVWTRTDAPVAAGQAGRSWYWGPAPGMVVREPFAGLPGGSHLVQYFDKSRMEINDPN
                     GDRNSKWFVTNGLLSIELISGRIQTGLTSYENRAPADIPLASDTDDTTAPTYASFLSV
                     SNTSRGDHPANAALGAAVTATIDKAGHVGQDAGRAMDATRIAYYEPKTHHNIPGVFWT
                     FLNATGPVREGGTTRTALLSDPWNFATGLPISEAYWARVKIAGAPQWVLIQAYERRVL
                     TFVPANAPHWRVQMGNIGQHYYQYRYGSAAATLTGAVARMAAAPSYHFESLIQLKSGN
                     LTVPYARQSGDFQAPDRTHFVQQSIEGTSEVITIGPTTYISDTTTGGRWQKLGAGTSS
                     GSIDLSSLFGVLQYAHDVTPASDETINGVPSRRLHMTLEPGVLPVVPGLSYSAATADV
                     YLAADNGRVTRLQTNVTLAPNATRQGFLVATVDFSRYGQAVDIREPAP"
BASE COUNT          467 a          940 c         1010 g          444 t
ORIGIN      
        1 ttacttcgca tccggtatat ccttctcctc agttaggcgg ctactatagc cggtacaggt
       61 gtatacgtga cgggcgtgac ccccgttacg ctccgcgcga gcgccggctt atctccagac
      121 gacggcgccc aggccgccaa acaagatcac cgccagggtg atcgccaggc aggccagcac
      181 gatcccgacc atgatcagcg ccccgctgcg ggcgttccag ggcgtgcgcg tctccggcgt
      241 gtcgtcgaag ccggcggaac cgggcgcggg acttgcctcc ttgcgcgtgg cgtagccggg
      301 gatatagtcg tcgtcgggtg gcgtggtgga catggggtac ctttccgcgc tagcgcaagc
      361 ccggctgcag gggcagatcc ggggtcgccg aggtcgccag cactccggtc agggcaatgc
      421 gcaggttcgc catcgccgtc agctcgatca gcgccaggat cagcgcgatc aagagcgggg
      481 tgagcgccat ctgccaggtc agcacgctgc ccgcgcgccg gctcatggtc gagacgatca
      541 gcacgttggt catggtcagc agcgccagca gcccgcccac gctgcccagt gccagcggcc
      601 agtagagcca gtcaccgatg cgcgtgtcgc ccgccgccga cagcatgatg ccgaccacga
      661 ccgcggttgg caccaggtag ccgaacaact cgaacggctg gtcgatggat cgttcgcggt
      721 gcaacagccc ggtgcgccag aagctctggt tgaacagcgg gtagagcacg aacatcagcg
      781 acactccggc cagcacgccg gtgatcagcc gcagccagtt cgtcgtctcg tacagcgccg
      841 tggcccccgg cagcgagtcg aaggtcgagt tgatgccgtc gaacaccatc gtcgagaaca
      901 tgaagccgag cagcgggaag atgcgccgcg aaggcaggcc cccggccagg ggccgcaaaa
      961 tcagcagcat gataaacgtg accagcgcgc ccaggtagat gccgctgcag cgggcgcaga
     1021 gcggcaactg gtgcccggcg atgaacagcg agtggctgct caactggtgg cagacgccga
     1081 agcccaggaa gtcgagcttg tacttcagcg aaccgggcac aaacaccagc aggcccagca
     1141 gcacggcgat cagccccgcc tgcaacgggg agatcggcag ggaccgctct ttcagcacgc
     1201 gcatcatggt gcgggctccc ggatgtcgac cgcctggccg tagcggctga agtcgacggt
     1261 ggcgaccagg aagccttggc gggtggcgtt gggcgccagc gtgacgttgg tttgcaggcg
     1321 cgtgacccgg ccgttgtccg cggccaggta gacatcggcg gtggcggcgg aatagctcag
     1381 gccgggcacc acaggcagca cgccgggttc cagggtcatg tgcagccgcc ggctgggcac
     1441 gccgtttatc gtctcgtcag aggccggggt cacatcgtgg gcgtattgca gcacgccgaa
     1501 caggctggac agatcgatgc tgcccgaact ggtccccgcc cccagctttt gccagcgccc
     1561 gccggtcgtc gtgtcgctga tataggtcgt ggggccgatg gtgatcactt cgctcgtgcc
     1621 ctcaatcgac tgctggacga agtgcgtgcg atccggcgcc tggaagtcgc ccgactggcg
     1681 ggcatagggc acggtgaggt tgccgctctt gagttggatc agtgactcga agtggtagct
     1741 gggcgccgcc gccatgcgtg cgaccgcgcc ggtcagcgtg gccgccgcgc taccgtagcg
     1801 gtactgatag tagtgctggc cgatgttgcc catctgcacg cgccaatgcg gcgcgttggc
     1861 cggcacgaag gtcagcacgc ggcgctcata cgcctggatc agcacccact gcggcgcccc
     1921 ggcgattttg acgcgcgccc agtaggcttc gctgatcggc agccccgtgg cgaagttcca
     1981 ggggtcggac agcagggccg tgcgggtggt gccgccctcg cgcaccggcc cggtcgcgtt
     2041 caagaaggtc cagaacacgc cggggatatt gtggtgcgtc ttcggctcgt aataggcgat
     2101 gcgggtggcg tccatggccc gcccggcgtc ctgccccacg tgcccggcct tgtcgatggt
     2161 cgccgtgacc gccgcgccca gggccgcgtt cgcggggtgg tcgccgcgcg aggtgttgct
     2221 gacggagagg aaggacgcat aggtgggcgc ggtcgtatcg tcggtgtccg aggccagcgg
     2281 gatatcggcg ggcgcccggt tctcgtagct ggtgaggccg gtctggatac gcccggagat
     2341 caactcgatg ctgagcaagc cgttggtgac gaaccacttc gagttgcggt cgccgttggg
     2401 gtcgttgatc tccatgcggc tcttatcgaa atactggacc agatgcgatc cgcccggcag
     2461 cccggcaaac ggttcgcgca cgaccatgcc gggggccggc ccccagtacc aggagcgccc
     2521 ggcctgcccg gcggccacgg gggcgtcggt gcgggtccag acttgctgaa acgccgggtc
     2581 ggcatagcgc aactcgacgg ctgccgcggg ccgcgccgcg agcacgcccg tgcccgcagc
     2641 cgtgccgagc agcaccgcca gcagccagcg cgcgccgcgc cggggggtgg ttcgatgcat
     2701 gagaatagcg tacctctcta gttgcacacc gtggggcgat ctcgcagcag gcgccctgaa
     2761 cgccctcatt atagccgctg cgcccgcccg gcggcaagga cccgaccggg gcgtgggtgc
     2821 gcggcatgat gttgcatccg ggcgcccggc caacctctcc c
//
LOCUS       DASUYC010000992         2168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1552257, whole genome shotgun sequence.
ACCESSION   DASUYC010000992 DASUYC010000000
VERSION     DASUYC010000992.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552257"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..499
                     /locus_tag="VF276_16225"
     CDS             74..499
                     /locus_tag="VF276_16225"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEX6608449.1"
                     /translation="MAVRITSQAVKLDTRIIPTYVQDGAWQVADYAAGWLYGTAQPGQ
                     PGNSVFAGHNNWRGEVFRYLEFFHIGDTFTITMSDGTTHRYRVTEMYKLPQANADRDQ
                     QIANARYADNTPDERVTLITCWPYTTYTHRLIIIGHPVP"
     gene            499..1074
                     /locus_tag="VF276_16230"
     CDS             499..1074
                     /locus_tag="VF276_16230"
                     /inference="COORDINATES: protein motif:HMM:TIGR04366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HEX6608450.1"
                     /translation="MSDHIPPDDPAAQELRPIDRNLLDLATTRARTNPRRRALLRYHE
                     FPEPVQRMLNAIEPDSYARPHRHVTPPKVEVFLVLRGRAIVVRFDDSGAILEAPEIAA
                     GGPRHGVEVPPGAWHMALALEPDTVFYEVKEGPYDPARDKTFAPWAPPEDDAVAGRRY
                     LEGIRRALRLPPLVAHLPDPTALDDEEDDLF"
     gene            1157..>2168
                     /locus_tag="VF276_16235"
     CDS             1157..>2168
                     /locus_tag="VF276_16235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608451.1"
                     /translation="MTQAPALQIADIAPAVLAALAGYRLTPAQIAALPKPVPNPEQGL
                     ITVEPAGVHANPTAFWWWRAYPFHMLSILALLDVPLLNGGEVADWIKRQQHPDGYFRP
                     DRLGHTVRQATYHACMMLRLLDAQADSAGWDRYRLINLLQSQYQPGARVPFARLPRDR
                     KDPNSEFEVILACVASLGALGDHLAEADAALRYLATRQEPAGTFRPRGWANTPDAPRE
                     AATAAAVRSFLLLGADPPHAARCAATIQGWQLVDGGFDAVFAQYPAYPQISHLVATAR
                     AVLTLDLLGSAPVDRESCLRRILQFWSPSGGFFADSLPVPAAGPDPGTLRATHHGLLA
                     LAA"
BASE COUNT          320 a          825 c          700 g          323 t
ORIGIN      
        1 gggcaacgtg ggccgccatt agtacgattg gcgctacggc accaatcgcc tgggccaggt
       61 cagcccgccg ccggtggccg tgcgcatcac cagccaggcc gtgaagctcg atacccgcat
      121 catcccgacc tatgtgcagg acggcgcctg gcaggtggcc gactacgccg cggggtggct
      181 ctacggcacg gcgcaaccgg gccagcccgg caacagcgtc ttcgccgggc acaacaactg
      241 gcgtggcgag gtgttccgct acctggagtt cttccacatt ggcgacacgt tcaccatcac
      301 catgagcgac ggcacgaccc atcgctaccg cgtcaccgag atgtacaagc tgccgcaggc
      361 caacgccgac cgcgatcagc agatcgccaa cgcccgctat gccgacaaca cgcccgacga
      421 gcgcgtgacg ctgatcacct gctggccgta caccacctat acccaccggc tgattatcat
      481 cggccacccg gtgccctgat gagcgaccat atcccgccgg acgaccccgc ggcgcaggag
      541 ttgcggccca tcgaccgcaa cctgctcgat ctggcgacga cgcgggcgcg caccaacccg
      601 cgccgccgcg ccctgttgcg ctaccacgag ttccccgagc ccgtgcagcg gatgctcaac
      661 gctatcgagc cggacagcta tgcccggccc caccgccacg tcactccgcc caaggtcgag
      721 gtgttcctgg tgctgcgtgg gcgggccatc gtcgtgcgct tcgacgacag cggggcgatc
      781 ctggaggcgc cggaaattgc cgcgggcggg ccgcgccacg gcgtcgaggt gccgcccggc
      841 gcctggcaca tggccctggc cctggagccg gacaccgtgt tctacgaggt caaggaaggc
      901 ccctacgacc ccgcccgcga caagacgttc gcgccctggg cgccgccgga ggacgacgcc
      961 gtggccgggc ggcgctacct ggaaggcatc cgccgggcgc tgcgcctgcc gcccctcgtc
     1021 gcccatctgc ccgaccccac cgccctggac gacgaagagg acgacctgtt ctagccgggg
     1081 cgccccggcc ttgacgcagc gggcgattcc gcttcggccc gcctgcctgg gatagaggga
     1141 gtcaggaggg ccgtgcatga ctcaggcacc tgcactccag atcgcggaca tcgctccggc
     1201 ggtgctggcg gccctggccg gctaccggct cacgcccgcc cagatcgccg cgctgccgaa
     1261 gccggtgccc aatcccgagc aagggctgat caccgtcgaa ccggcggggg tgcatgcgaa
     1321 cccgaccgcg ttctggtggt ggcgtgccta cccattccat atgctctcga tcctggcgct
     1381 gctcgatgtg cccctgctga acggtggcga ggtcgcggac tggatcaagc ggcagcaaca
     1441 ccccgacggc tacttccgtc cggatcggct cggccacacg gtgcgccagg cgacctatca
     1501 cgcctgcatg atgctgcggt tgctggacgc ccaggccgac agcgccggct gggaccgcta
     1561 ccgcctgatc aatctgctcc agagccagta tcagccgggc gcccgtgtgc ctttcgcgcg
     1621 cctgcctcgc gaccgcaagg accccaacag cgagtttgag gtcatcctgg cctgcgtggc
     1681 tagcctcggc gccctcggcg accatctggc cgaggccgat gcggccctgc gctatctggc
     1741 gactcgccag gaacccgccg ggaccttccg gcctcgcggc tgggccaata cgccggacgc
     1801 gccccgcgaa gccgcgactg ccgccgccgt gcgcagtttc ctgctgctgg gcgccgatcc
     1861 gccccacgcg gcgcgctgtg cggcgaccat ccagggctgg cagcttgtcg acggcggctt
     1921 tgatgccgtc ttcgcgcagt atcccgctta tccgcagatc tcgcacctgg ttgcgacggc
     1981 cagggcggtg cttaccctcg atctgctggg gtccgcgccc gtggatcgcg agtcctgcct
     2041 ccggcgcatc ctgcaattct ggagtccgag cggcgggttt ttcgccgact cgctgccggt
     2101 gcccgccgcc ggccccgatc cgggcaccct gcgagcgacg caccacggcc tcctcgccct
     2161 ggccgcat
//
LOCUS       DASUYC010000993         2761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1945489, whole genome shotgun sequence.
ACCESSION   DASUYC010000993 DASUYC010000000
VERSION     DASUYC010000993.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1945489"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..1278
                     /gene="nagA"
                     /locus_tag="VF276_16240"
     CDS             97..1278
                     /gene="nagA"
                     /locus_tag="VF276_16240"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEX6608452.1"
                     /translation="MLYIHHATILTPGELIAEGAVLIDGGRIVAAGPAVRIACPPGAQ
                     TIDATGLLLAPGFIDLQINGAFGHDFTADPATVWAVGAALPRYGVTAFLPTVITSPLE
                     TVAAAQAVLSQGPPADYAGAAALGLHLEGPFLNPAKRGAHNPAHLRPPDRAAVANWTP
                     ERGVRLVTLAPELPGALPVVQALVARGVVVSAGHSMATAEEAQAGFAAGITYGTHLFN
                     AMPPLDHRAPGLPGALLADPARTTGVIADGIHLHPATVAVAWQAKGATRLNLVTDAMG
                     ALGMPPGQYRLGDWDVIVDGRSARLADGRLAGSVLSLDEAVRNLIAFTGCPLEAALAA
                     VTTVPAAVLGQQGQRGQVAPGYAADLVLLTADLQVQATIVAGEVVYTATVAPVAMVAG
                     A"
     gene            1394..2431
                     /locus_tag="VF276_16245"
     CDS             1394..2431
                     /locus_tag="VF276_16245"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX6608453.1"
                     /translation="MGLRDEILEQPAVLQRWLDTQMDSVHRIADAIRAHDIDYVFLAA
                     RGTSDHAGVYAQYVWGARNRLPVALAAPSLFTMYAETPRLHHALVVGISQSGQSPDVT
                     GVIAEGRRQGTVTLALTNDVNSPLAQAAEFTLDVHAGQEKAVAATKTYTAELMAVAAL
                     SAALDQDDGDLAALRAVPGAVQAALGLEAEAERIATEQRAMEYCVVLGRGYNYGSALE
                     WALKLKELSYVLADAYSTADFQHGPIAILERGFPVLALAPDGAVFADQLALLRRLRDE
                     YGAQLLVISDRPEALALTSLALRLPDGVPEWLTPIVSIVPAQLYCYHLTRLKGHDPDH
                     PRTLHKVTLTE"
BASE COUNT          413 a          934 c          996 g          418 t
ORIGIN      
        1 aaagggcagc ggggcggcca ggaggcggcg cgggccggtt tcaaactggc ccctacaggg
       61 atgcgattat cgggaacggc tactagagga aatatcatgc tatacattca tcacgccacg
      121 atcctgacgc ccggcgaact catcgccgag ggggcggtgc tgatcgacgg ggggcgcatc
      181 gtggccgccg ggcccgccgt gcggattgcc tgcccgccgg gcgcgcaaac catcgacgcg
      241 accggcctgc tgctggcgcc gggcttcatc gatttgcaga tcaacggcgc ctttgggcac
      301 gatttcaccg ccgatccggc gaccgtctgg gcggtgggcg cggcgctgcc gcgctacggc
      361 gtgacggctt tcctgcccac ggtgatcacg tcgccgctgg agacggtggc cgccgcgcag
      421 gccgtgctga gccaggggcc gcccgcggac tatgcggggg cggcggcgct gggcctgcac
      481 ctggaggggc cgttcctcaa cccggccaag cgcggcgcgc acaacccggc gcatctgcgc
      541 ccgcccgacc gcgcggcggt ggcgaactgg acgccggagc gcggggtgcg gctggtcacg
      601 ctggcgcccg aactgccggg cgcgctgccc gtggtccagg cgttggtggc gcggggcgtc
      661 gtggtcagcg cgggccactc gatggcgacg gcggaggagg cgcaggccgg gttcgccgcg
      721 ggcatcacct acggcaccca cctgttcaac gccatgccgc cgctcgacca ccgggcgccg
      781 ggcttgccgg gcgcgctgct cgccgacccc gcgcgcacca ccggcgtgat cgccgacggc
      841 atccatctgc acccggcgac cgtcgccgtg gcctggcagg ccaagggcgc cacgcggctc
      901 aacctggtaa cggacgccat gggtgccctg ggcatgccgc cggggcagta tcgcctgggt
      961 gattgggatg tgatcgtgga cgggcgcagc gcccggctgg ccgacgggcg cctggccggg
     1021 agcgtgctca gcctggacga ggccgtgcgc aacctgatcg ccttcaccgg ctgcccgctc
     1081 gaagcagcac tggcggcggt aacgacggtg cccgcggcgg tgctgggcca gcagggccag
     1141 cgcgggcagg tcgcgcccgg ttacgccgcc gatctggtgt tgctcacggc ggacttgcag
     1201 gtgcaggcca ccatcgtggc gggcgaggtg gtgtatacgg ctactgtggc gcccgttgcc
     1261 atggtggcgg gagcatagtg ccgcttcaag aatcctgttg gggagcacac accggcgata
     1321 aacgagtacg ggcgcgataa atcgcgccgc tacacacaag aggtcacttg aagcagcaat
     1381 aggagggggc gccatgggac tgcgcgacga gattctggag cagccggcgg tgttgcagcg
     1441 ctggctggac acgcagatgg actcggtcca ccgcatcgcg gacgccattc gggcgcacga
     1501 catcgactat gtattcctgg cggcgcgcgg cacctcggac cacgcggggg tgtacgcgca
     1561 atatgtgtgg ggcgcgcgca accgcctgcc ggtggccctg gccgcgccgt cgctgttcac
     1621 tatgtacgcc gagacgccgc gcctgcacca cgcgctggtg gtcggcatct cccagtcggg
     1681 ccagtcgccc gatgtcaccg gggtgatcgc cgagggccgc cggcagggca cggtgacgct
     1741 ggcgctgacc aacgatgtca actcgccgct ggcccaggcc gccgagttca cgctcgatgt
     1801 ccatgcgggg caggaaaagg ccgtggcggc caccaagacg tacaccgccg agttgatggc
     1861 ggtggcggcg ctgtcggcgg cgctggacca ggacgacggt gatctagccg cgctgcgcgc
     1921 cgtgccggga gcggtgcagg ccgcgctggg gctggaagcc gaggccgagc gcatcgccac
     1981 cgagcagcgg gccatggagt attgcgtggt gctgggacgc ggctacaact acggctcggc
     2041 gctggaatgg gcgctcaagc tgaaggaact gtcctacgtg ctggccgatg cctactcaac
     2101 cgccgatttc cagcacggcc ccatcgccat cctggagcgc ggcttcccgg tgctggcgct
     2161 ggcccccgac ggcgcggtgt tcgccgacca actggcgctg ctgcgccgcc tgcgcgacga
     2221 gtacggcgcg caactgctgg tcatctccga ccggcccgaa gccctggccc tgacctcgct
     2281 ggcgctgcgc ctgcccgacg gcgtgccgga gtggctgacg cccatcgtca gcatcgtgcc
     2341 cgcgcaactc tactgctacc acctcacccg gctcaagggc cacgaccccg accacccacg
     2401 cactttacat aaggtaacac taaccgagtg agccgggtcg ccttgccggc tttgcgcccg
     2461 cccggtctgc cggacgggcc tttttttgcc tcctcgcgtt ctcgctgccg catccggccg
     2521 ggccgcctga tagttgcgcg gcctcttgct gcccgcaaga ccggcacgcg ggagaggatt
     2581 gattgaggtg ccggcaagcc aggctgcacc agcgatatca acatccggaa ttgtaacgat
     2641 ccgtgtaccg tgttccacgg ggatatttac ttatcatgtg taaaataagg agtgaagcga
     2701 atgcgagcgg gtggtcgctc tgcgggctgg cgcaagcgtc tcgcgtagct ccaggggtcc
     2761 a
//
LOCUS       DASUYC010000994         2506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_165853,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000994 DASUYC010000000
VERSION     DASUYC010000994.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165853"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="VF276_16250"
     CDS             <1..1049
                     /locus_tag="VF276_16250"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEX6608454.1"
                     /translation="TLLNPAQGHFGDVPPGSAFYQVVETAHAHGIISGYSDGSFRPGN
                     PATRAQIAKIVYLTLQSGCAMFPPDNIWNRDISTLPVHPLSDTYIASIGLSGHLHADF
                     GSGTYQGAPIGIPWLAVAGSEPLVPVSFTKPGESDPGPYPIPLTAPIEGGPASSGDRH
                     VLVVDQGGGCSLNELYLAYPQGNGSWQAYSGARWDLSSNALRPDDWTSADAAGLPILP
                     GLIRYDEVAAGAIRHAVRFTVSRSQNTHLWPARHDAGSADASLPPMGLRVRLKAGFDI
                     SGYPPAAQVILTALKQYGMFVSDNGSNWFLSGTQDERWDNDVLHTLSTVAGSNFEAVD
                     ESGLMIDPNSGASR"
     gene            1275..>2506
                     /locus_tag="VF276_16255"
     CDS             1275..>2506
                     /locus_tag="VF276_16255"
                     /inference="COORDINATES: protein motif:HMM:NF026177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX6608455.1"
                     /translation="MVPPQPHTPSPRTPIAWLTRIATRRLRYQIVIPYLLLAMVLAAG
                     ATYLLTRAASQSLHDRFHQQLLDAARGGANSLAGAESHQLAGLRLMLFTQGLPEAVRE
                     RDTTTLHSLIAPLALNNGFDHVLIVAADGNTLLDLHQPGAAPGPAISAEIPPTNLIQA
                     ALHPGAIDKISAVVDSTDGRGFYTAGPVRRGDQVIGAILVGTYLPRLLGQVQRDSLSN
                     AATFYGPQGVPLAHTLAGQPNDAGMVPPLPGGWYDTVHNDPSAPVEFRTVALHGDTFL
                     EATGAVPGHGLNGAPGVYGVMLSTSTLDAGLNNSLWTLVPFFGLGFILIVVIGGILAA
                     QIDRPVAQLVQATWAVARGDLEVQVPVVRHDELGLLAARFNDMIAGLRQLLFVKDLFG
                     RFVSPEVSAQLIAGQIKLG"
BASE COUNT          396 a          926 c          773 g          411 t
ORIGIN      
        1 ggaccttgct caatccggcg cagggccatt tcggcgatgt gccacccggt tccgccttct
       61 accaggtggt ggagacggcc cacgcgcacg gcatcatcag cggctacagc gacggcagct
      121 tccgccccgg caacccggcc acccgcgccc agatcgccaa gatcgtctac ctgacgctgc
      181 aatccggctg cgcgatgttc ccgccggaca acatctggaa ccgcgacatc tccaccctgc
      241 cggtgcatcc cctgtcggat acctacattg ccagcatcgg gctgagcggc cacctgcacg
      301 ccgatttcgg gtcgggcacc taccagggcg cgcccattgg tatcccctgg ctggccgtgg
      361 ccggcagcga gccgctggtg cccgtcagct tcaccaaacc gggcgagagc gaccccggcc
      421 cctaccccat cccgctgacc gcgcccatcg agggcggccc ggcgagcagc ggcgaccgcc
      481 acgtgctggt ggtggaccag gggggcggat gcagcctcaa tgagttgtac ctggcctacc
      541 cgcagggcaa cggaagctgg caggcctatt cgggcgcgcg ctgggacctg agttccaacg
      601 ccctgcgccc cgacgactgg acctccgccg acgccgcggg gctgccgatc ctgcccggtc
      661 tgatccgcta tgacgaggtg gcggcgggcg cgatccgcca tgccgtccgc ttcaccgtca
      721 gccgcagcca gaacacccac ctctggcccg cccgccacga tgccggcagc gccgacgcat
      781 ccttgccccc gatgggcctg cgcgtgcgcc tcaaggccgg cttcgacatc tccggctacc
      841 cgcccgccgc ccaggtgatc ctgaccgcgc tcaagcagta cggcatgttc gtctccgaca
      901 acggcagcaa ctggttccta tcgggcacgc aggacgagcg ctgggacaac gacgtgctgc
      961 acacgctgag caccgtggcc ggttccaact tcgaagcggt ggacgagtcc ggcctgatga
     1021 tcgaccccaa ctcgggcgca agccggtaga aggacgagtc cttacgaata tttagctttt
     1081 tctaatgatc aaaaccttac gataatgcag cttttgctct tgcagtgttc gtccgttgtg
     1141 gtataatcta cctgccatga atggaccgcg ctgcgtcggg ccgcggtgcg cttcaccatt
     1201 cacctgtgac gggcattatt ttcagactcc acccagcgca tcctcgcaac acaaatcgat
     1261 actgagaggg actcatggtg ccgccccagc cgcacacgcc ttccccgcgc acgccaatcg
     1321 cctggcttac ccgcatcgcc actcgccgcc tgcgctatca aattgtcatt ccgtatctat
     1381 tgctggccat ggtcctggcc gccggggcca cgtatctcct gacccgcgcc gccagccagt
     1441 cgctgcacga ccggtttcac cagcaattgc tcgacgccgc tcgcgggggc gccaatagcc
     1501 tggccggcgc cgagtcgcac cagttggccg ggctgcggct catgctcttc acccagggtt
     1561 tgccggaggc ggtgcgcgag cgcgacacga cgacgctgca cagcctgatc gcgccgctgg
     1621 cgttgaacaa cggcttcgac catgtgctga tcgtggccgc cgacggcaac accctgctcg
     1681 acctgcacca acccggcgcg gcgcccggcc cggcgatctc ggcggagatc ccgcccacga
     1741 acctgatcca ggccgcgctg caccccggcg ccatcgacaa gatctcggcg gtggtggaca
     1801 gcaccgacgg gcgcggcttc tacacggcgg ggccggtgcg ccgcggcgac caggtgatcg
     1861 gcgccattct cgtcggcacg tacctgccgc ggctgctggg ccaggtccag cgcgacagct
     1921 tgagcaatgc cgcgaccttc tatggcccgc agggcgtccc gctggcccac accctggccg
     1981 gccagcccaa cgacgcgggg atggtgccgc cgttaccggg cggctggtac gataccgtcc
     2041 ataacgatcc cagcgcgccg gtcgagttcc gtaccgtcgc gctgcacggc gacacgttcc
     2101 tggaagccac gggcgcggtg ccgggccacg gcctcaacgg cgcccccggc gtctacggcg
     2161 ttatgctcag cacgagcacg ctggacgccg gcctcaacaa cagcctctgg acgctggtcc
     2221 cgttcttcgg cctcggcttc atcctgatcg tggtcatcgg cggcatcctg gccgcgcaga
     2281 tcgaccgccc ggttgcccaa ctggtgcagg ccacctgggc cgtggcccgc ggcgacctcg
     2341 aagtgcaggt gcccgtggtg cgccacgacg agctgggcct gctggcggcg cgcttcaacg
     2401 acatgatcgc cgggttgcgg cagttgctgt tcgtcaaaga cctcttcggc cgctttgtct
     2461 cgccggaagt ctcggcccaa ctgatcgccg ggcagatcaa gctcgg
//
LOCUS       DASUYC010000995         2432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1783040, whole genome shotgun sequence.
ACCESSION   DASUYC010000995 DASUYC010000000
VERSION     DASUYC010000995.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1783040"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VF276_16260"
     CDS             complement(<1..201)
                     /locus_tag="VF276_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007701312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6608456.1"
                     /translation="MIGTPVSVVIPTYNRAELLRAALESVQAQTAPVAEVVVVDDGST
                     DDTAAVVAEFADRGLPMRYLAEP"
     gene            complement(198..1136)
                     /locus_tag="VF276_16265"
     CDS             complement(198..1136)
                     /locus_tag="VF276_16265"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX6608457.1"
                     /translation="MSLPVRARLRARLKRGAHRFVFPAATRGTGALFRLAGRVARPAV
                     RYVPPRGSERVLVLAPHPDDEALGCGGTIALHSRAGDAVCVVIVTDGGRSRAGGRGRA
                     EIRALRWSEAQAAVARLGPVDLRQWNLPEGRWAPEALDTRLAQVLAEFAPTLVYTTSC
                     VDFHPEHRRVAESLGRVLAAGAGRTCRAVRVYELQVPLTPVLANVAVPIGPVAAAKVR
                     ALAAYGTQAASFGWLPRQAGYLRRLYGARGPVEVFWELAPAQFVRLMAGYEPLGGRFR
                     GLRLRPFSDMLAWTIGLRARRRLRALAEGRAGGGTV"
     gene            complement(1133..2236)
                     /locus_tag="VF276_16270"
     CDS             complement(1133..2236)
                     /locus_tag="VF276_16270"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6608458.1"
                     /translation="MESVPPDFTPSWTVRPYEPGDETQLVDLYARVFGRPRSIEVWRW
                     KLQGHPAPYPTVWVAATGEGQIVGQYGGIPLGLQIGGQVYPAVHAVEAMTDPAFRRQG
                     MLTRLGEAAHGGWAAAGQVAVLGLPNDQWGTRNYALGYRPLFPLAWLRFPLHAERVVA
                     RSAQIPRPLAGPVAALGAAGSGVWRGLARGAMHRQMRGTAVAVEEVTAPDPALDRLWA
                     RVAPHYPHLVVRDSGWVDWRFLRAATGAYRVLLARAGGEPVAYLAYRLVESAERVTGY
                     VADVFMTPDGAAGATALLDAALRDLAGRGAATVLATGVPGSALYGLLRACGFWPMRHG
                     FQFEIVPLAPLIQPTALADPRAWYLTAGDFDVI"
BASE COUNT          376 a          954 c          836 g          266 t
ORIGIN      
        1 cggttccgcc aggtagcgca tcggcaagcc ccgatccgcg aactcggcca ccaccgccgc
       61 cgtgtcgtcg gtcgagccgt cgtccaccac gacgacctcc gcgaccggcg ccgtctgcgc
      121 ctgcacgctc tccagcgccg cccgcaacag ctcggcgcgg ttgtaagtgg gaatcaccac
      181 gctcacgggc gtgcctatca taccgtgccc cctccggccc gcccttccgc cagcgcgcgc
      241 aagcggcgcc gggcgcgcaa accgatcgtc caggccagca tatcgctgaa tgggcgcaag
      301 cgcaacccgc gaaaccgccc cccgagcggc tcgtagcccg ccatgaggcg gacgaactgc
      361 gccggggcca gttcccagaa cacctcgacc ggcccgcgcg ctccgtacag ccgccgcagg
      421 tagcccgcct ggcgcggcag ccagccgaac gaggccgcct gcgtgccgta ggcggccagg
      481 gcgcggacct tggcggcggc caccggcccg atgggcacgg ccacgttcgc cagcacgggc
      541 gtcagcggca cctgtaattc gtacacgcgc acggcgcggc aggttcggcc cgcgcccgca
      601 gccagcaccc gccccaggct ctcggccacc cggcgatgct cgggatgaaa gtcgacacag
      661 gacgtagtat agaccagcgt gggcgcgaac tcggccagca cttgcgccag ccgggtgtcc
      721 agcgcctccg gcgcccaacg gccctccggc agattccact ggcgcagatc gacaggccct
      781 agccgcgcga ccgccgcctg cgcctcggac cagcgcaaag cccggatctc cgcgcgcccg
      841 cgcccgcccg cccgcgaccg gcccccatcg gtcacaatga cgacgcacac ggcatcgccc
      901 gcccggctgt gcagggcgat ggtcccgccg cagcccagcg cctcgtcgtc ggggtgcggg
      961 gccaggacca ggacgcgctc gctcccgcgc ggcggcacgt agcgcacggc agggcgggcc
     1021 acgcgacccg cgaggcgaaa cagcgccccg gtgccgcggg tcgccgccgg gaagacgaag
     1081 cggtgggcgc cgcgcttcag ccgggcgcgc agccgggcac gcacgggcag gctcatatca
     1141 cgtcgaaatc gcccgcggtc aggtaccagg cgcgcggatc ggccagcgcc gtgggctgga
     1201 tgaggggcgc gaggggcacg atctcgaact ggaagccgtg gcgcatgggc caaaagccgc
     1261 aggcgcgcaa caggccgtac agcgccgacc ccggcacgcc ggtcgccagc accgtggcgg
     1321 cgccacgacc ggcaaggtcg cgcagggcgg catcgagcag cgccgtcgcc cccgccgcgc
     1381 cgtccggcgt cataaacacg tcggccacgt agcctgtcac gcgctccgcc gactcgacca
     1441 ggcgataggc caggtaggcc accggttcgc cgcccgcccg cgccagcagc acccggtacg
     1501 cgccggtcgc cgcgcgcagg aaccgccaat cgacccagcc gctgtcgcgc accaccaggt
     1561 gcgggtagtg cggcgcgact cgcgcccaga gccgatcgag agccggatcg ggcgccgtca
     1621 cctcttccac cgcgacggcg gtgccgcgca tctgccggtg catggccccg cgcgccaggc
     1681 cgcgccacac ccccgacccc gctgcgccga gcgccgccac cggcccggcc agcgggcgcg
     1741 ggatctgcgc gctacgcgcg acgacccgct cggcgtgcaa cgggaagcgg agccaggcca
     1801 gcgggaagag tgggcggtag cccagcgcat agttgcgcgt gccccactgg tcgttgggca
     1861 ggcccagcac cgccacctgc cccgccgccg cccagccgcc gtgggccgcc tcgcccagcc
     1921 gggtcaacat gccctggcgc cggaacgcgg ggtcggtcat cgcctccacg gcatggaccg
     1981 ccgggtagac ctgcccgccg atctgcaagc ccagcgggat gccgccgtac tggccgacga
     2041 tctgcccctc gccggtcgcg gcgacccaga ccgtggggta gggcgccggg tggccctgca
     2101 gcttccagcg ccagacctca atcgaccggg gccggccaaa gacgcgggca tagaggtcga
     2161 ccagttgcgt ttcgtcgccc ggttcatatg ggcgcacggt ccacgagggc gtaaagtcgg
     2221 gcggcacgga ttccacgggc aacgtctccg ctacaggcag gccggcgcgg tcccggcgac
     2281 cggcggcagc aatcccgtcc agtatagcca gcaccgcgta acggcacaag ggggatccgt
     2341 cgcgaggcag gcgaccggtt cccctcggtt gtataggctt tgtgccatct gtagttgcag
     2401 ttggacacga attaggctac aattgtccca gt
//
LOCUS       DASUYC010000996         3560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_165963,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000996 DASUYC010000000
VERSION     DASUYC010000996.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165963"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VF276_16275"
     CDS             complement(<1..234)
                     /locus_tag="VF276_16275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608459.1"
                     /translation="MDDKRYPSRPAPVVRPGNGPPRPAGAARTAPGPATRVQESTPAY
                     VPEVRIPIKIAAIGAPNTTQEFTVDILEDFIGET"
     gene            complement(285..1406)
                     /locus_tag="VF276_16280"
     CDS             complement(285..1406)
                     /locus_tag="VF276_16280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HEX6608460.1"
                     /translation="MAHESDLTLPFDLVTSMVETIEGCAARCQATLDRISPDLDAVRV
                     RLLDIFPGLIRPVPPAAPIAIGAVDGSHYAVETLGTAFAGVCAIRVDPDGRPDHRLLA
                     ENLPHYPELDLALAGLRTIFEITLLRGATPDCLLLLDGSYWSALQAINRMLDLCDSTR
                     GRTDLWAPLLPHLEPFFRDGVLEEMLLRGCVVANSKQTVSRRFVSAVYGAPAADGSGI
                     PATNDRWLFSVVLQPGEYTAPLRLMEDTDTRVGGGAWYAREPGQLGWTRRQRDEIDRA
                     YEDLYVVYYRPHDWGNALRLELPGWLAADDALPGVLTGLRDQIVSPDVQEPRTQFIAD
                     VLCKQLPAMLDAIATGTINAIIPRYDPELVHRFFGPYRT"
     gene            1679..2743
                     /locus_tag="VF276_16285"
     CDS             1679..2743
                     /locus_tag="VF276_16285"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HEX6608461.1"
                     /translation="MKGLILSGGKGTRLRPITYTSAKQLVPVANKPVLFYGIEYMVEA
                     GITDIGIVVGDTREEIKNAVGTGERWGANITYIPQEAPLGLAHAVKIAEPFLGSDRFV
                     MYLGDNMIEGGFASFVNEYVEGGYNCEVLLKRVPNPQSYGVAVVEGGRVLRLVEKPRE
                     PISDLALVGVYMFDPNVFEAVNSIKPSGRGELEITDAIQYLLDHGYSIHAHELDGWWF
                     DTGKMEDMLEVNRLVLDSLEPCVQGYADSESQILGKVTLAKTAEVINSVIRGPVIIGE
                     HTRIVNSYIGPFTAIHYGCTVTNSEIEHSIILENSQLLDLPGRLENSLIGRNVEIGRS
                     PIKPKAYKMILGDHSKVGVL"
     gene            2743..>3560
                     /gene="rfbB"
                     /locus_tag="VF276_16290"
     CDS             2743..>3560
                     /gene="rfbB"
                     /locus_tag="VF276_16290"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEX6608462.1"
                     /translation="MKNLLITGGAGFIGSNFVRMMLEKYPDYHLTVYDKLTYAGNLDN
                     LRDVQDNPRYSFVHGDIGDAALVEETLREHNIDTIVNFAAETHVDRSIQEPGSFIQTD
                     VYGTYVLLEATRKLGLERMLQVSTDEVYGSVPEGSSTERDNYDPRSPYSASKAGGELM
                     CHAYFETFHTPVVITRGSNNYGPYQYPEKLIPLFITNLLDGKPVPVYGDGMNVRDWIY
                     VLDHCSGIDMVLHQGVLGEAYNVGGGNEKPNIEITRLLLDLTGRGPDMITYVADR"
BASE COUNT          664 a         1152 c         1155 g          589 t
ORIGIN      
        1 cgtctcgccg ataaagtcct ccaggatgtc gactgtgaac tcctgcgtcg tattgggcgc
       61 gccaatcgcc gcgatcttga tcgggatgcg tacctccggc acatacgcag gcgtgctctc
      121 ctgcacccgt gttgcggggc cgggcgccgt ccgtgccgcc ccggcggggc gcggcggccc
      181 attccctgga cgcacaaccg gggcagggcg cgagggatag cgtttgtcgt ccaagggggg
      241 tcctccttta cacaatgact ctaatggtgt ctacgcagta cttatcacgt tctgtaagga
      301 ccaaagaagc gatgcaccaa ctccgggtcg tagcgcggga ttatggcgtt gattgtgccg
      361 gtggcgatgg cgtcgagcat ggccgggagt tgcttgcaga gcacgtcggc gatgaactgc
      421 gtgcgtggct cctgcacgtc gggcgagacg atctggtcgc gcaggccggt cagcacgccg
      481 ggcagcgcat cgtccgccgc cagccagccg ggcagttcca ggcgcagggc gttgccccag
      541 tcgtgcggcc ggtagtagac cacgtacaga tcctcatacg cccggtcgat ctcgtcgcgc
      601 tggcggcgcg tccagcccag ttggcccggc tcccgtgcat accaggcgcc gccgcccacc
      661 cgcgtgtcgg tgtcttccat caggcgcaga ggcgccgtgt attcgcccgg ctgcaagacg
      721 accgagaaca gccagcggtc gttggtggcc gggatgccgc tgccatccgc cgcgggcgcc
      781 ccgtagacgg cgctgacgaa gcggcgcgag acggtctgct tcgagttggc gaccacgcag
      841 ccgcgcagca gcatctcttc cagcacaccg tcgcggaaga agggctccag gtgcggcagc
      901 agcggcgccc agagatccgt ccgcccccgc gtactgtcgc acaggtcgag catgcggttg
      961 atcgcttgca gggcgctcca gtaggacccg tcgagcagca gcaggcaatc gggcgtggcc
     1021 ccacgcagca gggtgatctc gaagatggtg cgcagcccgg ccagggccag gtcgagttcg
     1081 gggtagtgcg gcaggttctc ggccagcagg cggtggtcgg gccgcccgtc cgggtccacg
     1141 cggattgcgc agaccccggc gaaggccgtg cccagcgtct ccacagcgta atggctgccg
     1201 tccacggcgc cgatggcgat gggcgcggcg ggcggcacgg ggcggatcag gccggggaag
     1261 atgtccagca ggcggacgcg caccgcgtcg aggtcgggac tgatgcggtc gagggtggcc
     1321 tggcagcgcg cggcgcagcc ctcaatagtc tccaccatcg aggtaacgag gtcgaacggc
     1381 agcgtcaggt cggactcgtg cgccataagg gtgctcctgg cccgcgcccg gcgcggggga
     1441 ccggcagtcg gctatacata attatagaac gagtgtgcga acccgtcaat gccgccgggc
     1501 cgggcgccgt ggcaacggaa cccggtttcc gctcgatggt tatactactg tggattcgcg
     1561 cgtccaacca caggcgtttt cgcgcgggca cgcgcgccct tgcttgccat gggaagcttt
     1621 catatggtat gcttggcacc gagtgtctcg gaaatccagc aagcggagga gtcttatatt
     1681 gaaagggtta atcctgagcg gcgggaaggg cacacggctg cgcccgatca cctacaccag
     1741 cgccaagcaa ctggtgcctg tcgccaataa gccggtgctg ttctacggca tcgagtacat
     1801 ggtcgaagcg ggcatcacgg acatcgggat tgtcgtcggc gacacccgcg aggagatcaa
     1861 aaacgccgtc ggcacgggcg agcgctgggg ggccaatatt acctacatcc cccaggaagc
     1921 gcccctgggc ctggcccacg cggtgaagat cgccgagccg ttcctcggca gcgaccgctt
     1981 cgtcatgtac ctcggcgaca atatgatcga gggcgggttc gcctcgttcg tgaacgagta
     2041 cgtggagggc ggctacaact gcgaagtgct gctcaagcgc gtgcctaatc cccagtccta
     2101 tggcgtggcg gtggtcgaag gtggccgggt gctgcgcttg gtcgagaagc ctcgcgagcc
     2161 gattagcgac ctggcgctcg tcggcgtcta catgttcgac cccaacgtgt tcgaggccgt
     2221 gaacagcatc aagccctcgg gccgcggcga actggagatc accgacgcca tccagtatct
     2281 gctcgaccac ggctatagca tccacgccca cgaactcgac ggctggtggt tcgataccgg
     2341 caagatggaa gacatgctgg aagtgaaccg cctggtgctc gacagcctgg aaccctgcgt
     2401 ccagggctac gccgactccg aatcgcagat cctgggcaag gtcacgctcg ccaagaccgc
     2461 cgaggtgatc aactcggtga tccgcgggcc ggtgatcatc ggcgaacaca cgcgcatcgt
     2521 caattcctac atcgggccgt tcaccgccat ccactacggc tgcaccgtca ccaacagtga
     2581 gattgagcac tcgatcatct tggagaactc gcagttgctg gacctgccgg gccgcctcga
     2641 aaacagcctc attggccgca acgtcgaaat tggccgctcg ccgatcaagc ccaaagccta
     2701 taagatgatc ctcggcgatc acagcaaagt aggagtgctc taatgaagaa cttgctgatc
     2761 accggcggcg ccgggtttat cggcagcaat ttcgtccgca tgatgctgga gaagtacccc
     2821 gattatcacc tcacggtcta cgacaagctg acctacgccg gcaacctcga caacctgcgc
     2881 gatgtccagg ataacccgcg ctacagcttt gtccacggtg acatcggcga cgccgcgctg
     2941 gtcgaagaga cgctgcgcga gcacaacatc gacaccatcg tcaacttcgc cgccgagacc
     3001 cacgtggacc gctcgatcca ggagccgggc agcttcatcc agaccgatgt ctacggcacc
     3061 tacgtgctgc tcgaagcgac gcgcaagctg ggcctggagc gcatgttgca ggtctcgacc
     3121 gacgaagtct acggcagcgt gcccgaggga tcgtccaccg agcgcgacaa ttacgacccg
     3181 cgcagcccct actcggccag caaggcgggc ggggaactca tgtgccacgc ctatttcgag
     3241 accttccaca cgccggtggt gatcacccgc ggctcgaata actacggccc ctaccagtac
     3301 ccggagaagc tgatcccgct gttcatcacc aacctgctcg acggcaagcc ggtgccggtc
     3361 tacggcgacg gcatgaacgt gcgcgactgg atctacgtgc tcgaccactg ctcgggcatc
     3421 gacatggtgc tgcaccaggg cgtgctgggc gaagcctaca acgtcggcgg cggcaacgag
     3481 aagccgaaca tcgagatcac caggctgctg ctcgacctga cgggccgcgg ccctgacatg
     3541 atcacttacg tcgccgaccg
//
LOCUS       DASUYC010000997         2143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1553176, whole genome shotgun sequence.
ACCESSION   DASUYC010000997 DASUYC010000000
VERSION     DASUYC010000997.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1553176"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VF276_16295"
     CDS             complement(<1..325)
                     /locus_tag="VF276_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015121138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEX6608463.1"
                     /translation="MPGTYTFPGVYIEEIPSGVHTITGVSTSDTAFVDFFAQGPVDTA
                     VRCTSFADFERQFGGLDSRSEASYAIQQYYLNGGSIAWVIRAVAANAESATRALPRSA
                     GYYASG"
     gene            complement(336..>2143)
                     /locus_tag="VF276_16300"
     CDS             complement(336..>2143)
                     /locus_tag="VF276_16300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6608464.1"
                     /translation="EPPGAHPGVAGCSLVYHKAAWAVHRFPETRRGEDSAFLRQFPAE
                     RVAATTDSSYYIALIHPGNTAARNLADPCWQRRPLEEVTARLALDRDFYVALRNGRGA
                     APAVRPALEPVTVAAPFMVYDGYGSLSEYVVRGMVQAGAAVHVTPIRIDPAGLSDEIG
                     AILARSRPEPGAVTLCFSWPRENLAPFHAARDLFIYTMWETSALPAGWADVLNPARAV
                     MVPSRFVARTFRESGVTVPVEVVPHGVDPEVYHYEDRPERPGVTTLIVGTFVPRKNIH
                     IGIAAWKQAFAGEPEARLIIKTRFRVTPYVPDDPRITFVDTEERTHGIAHWYRQADVL
                     LALGNEGFGLPLVEGMATGLPVIALDSEGQSDVCSEAADCVLPVPPTRWQVFDEAPFG
                     RCGVRGIPAVEDVAERLRWVAGHRAEAQALGRAASEWTLAQRNIWAMGPALLAAMERH
                     TRPARPLRRRYTLWVAPGSPATVTAGYTAALAAALPAAQVTTQPPDLRSVRVLHIQHE
                     DGAWDEAALTQVVQQARYAGVPVAVTEHRAGPVARPWERDAAVLVALTADDAALLRAR
                     WPAKRVELLPLDPEASRPRTAAAHLALWRTLESSS"
BASE COUNT          325 a          728 c          794 g          296 t
ORIGIN      
        1 cgccggatgc atagtagccg gcgctgcgcg ggagggcgcg cgtggcggac tcggcgttgg
       61 cggcgaccgc gcggatgacc caggcgatgc tgccgccgtt gagatagtat tgctggatgg
      121 cgtagctcgc ctcgctacgg ctatccagcc caccaaactg gcgctcgaaa tcggcgaagc
      181 tggtgcagcg caccgccgta tcgacaggcc cctgcgcgaa gaaatcgacg aaggccgtgt
      241 cggacgtgct gactccggta atggtgtgca cgccgctcgg gatctcctcg atgtagacgc
      301 cggggaaggt gtatgtgccg ggcatagttc ctcctttacg aactgctttc cagggtgcgc
      361 caaagggcca aatgggccgc tgccgtgcgc ggccggcttg cctcgggatc gagcggcaga
      421 agttcgaccc gcttcgcggg ccagcgagcc cgcaacagcg cggcgtcgtc cgcggtcagc
      481 gcgaccagga cggcggcgtc acgctcccag ggccgcgcca ccgggcccgc gcggtgctcg
      541 gtgacggcga cgggcacgcc ggcatagcgc gcctgctgca ccacctgggt gagggcagct
      601 tcgtcccagg cgccgtcctc gtgctggatg tgcagcacgc gcacgctccg caaatcgggc
      661 ggctgggtcg tcacctgggc ggcgggcaac gcggcagcca gggccgctgt gtagcctgcg
      721 gtaacggtcg cgggcgaacc gggggccacc cagagcgtgt agcgccgccg cagggggcgg
      781 gcaggccggg tgtggcgctc catagccgcc agcaatgccg ggcccatcgc ccagatattg
      841 cgctgggcca gcgtccactc ggatgcggcg cgacccaacg cctgggcttc ggcgcggtgc
      901 ccggctaccc agcgcagccg ctcggctaca tcctcgacgg ccgggatgcc gcgtacgccg
      961 cagcgcccga acggcgcctc gtcgaacacc tgccagcgcg tgggcggcac cggcagcacg
     1021 caatcggcgg cctcgctaca cacatccgat tggccttccg agtccagggc gatcaccggc
     1081 aggccggtcg ccataccctc gaccagcggc aggccgaagc cctcgttgcc cagcgccagc
     1141 agcacgtcgg cctggcgata ccagtgggcg atgccgtgcg tgcgctcctc cgtgtccacg
     1201 aaggtgatgc gcggatcgtc gggcacgtaa ggcgtgacgc ggaagcgggt cttgatgatc
     1261 agccgggcct ccggctcgcc cgcgaacgcc tgtttccagg cggcgatgcc gatgtggatg
     1321 ttcttgcgcg gcacaaacgt gcccacgatc agcgtggtga caccggggcg ctccgggcgg
     1381 tcctcgtaat ggtagacctc gggatcgacg ccgtggggca ccacttcgac cggcaccgtg
     1441 acgcccgact cgcggaaggt gcgggccacg aaccgcgagg gcaccatgac ggcgcgggcc
     1501 gggttgagca cgtcggccca accggcgggc agcgcgctgg tctcccacat ggtgtagatg
     1561 aacagatcgc gggcggcgtg gaacggggcc aggttttcgc gcggccagga gaagcagagc
     1621 gtgacggcgc ccggttcggg gcgcgagcgg gccaggatgg ccccgatttc gtcggacagc
     1681 cccgccgggt cgatgcggat cggcgtgacg tgcaccgccg ccccggcctg gaccatgccg
     1741 cgcaccacgt actcggagag gctgccgtag ccgtcgtaga ccatgaacgg cgccgccacc
     1801 gtgaccggct ccagggccgg gcggacagca ggcgccgcgc cgcgcccatt gcgcagcgcg
     1861 acataaaagt cgcggtcgag ggccagccgg gcggtgactt cctccagtgg ccggcgctgc
     1921 cagcagggat cggctaggtt gcgcgcggcg gtattgccgg gatggatcag cgcgatatag
     1981 taagacgagt ccgtcgtggc ggcgacgcgc tccgcgggga actggcgcaa gaacgcgctg
     2041 tcctcgccgc gccgggtttc ggggaagcgg tgcacggccc aggccgcctt gtgatagacc
     2101 aggctgcacc cggctacgcc gggatgggca ccgggtggct cct
//
LOCUS       DASUYC010000998         3666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1159279, whole genome shotgun sequence.
ACCESSION   DASUYC010000998 DASUYC010000000
VERSION     DASUYC010000998.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159279"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(384..926)
                     /locus_tag="VF276_16305"
     CDS             complement(384..926)
                     /locus_tag="VF276_16305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608465.1"
                     /translation="MAALADELREHATAILDELMHRAPQELKGTLYDEMNKQKILRAR
                     YANVLEVITDYVQTQDVALFRQHHIIIFMQRYYLDIDAGNPLTPRLINWRTREIERSV
                     NMYLEVLKQFTSPKYHTQLETIFGSVRLLIHQMGTFLLVNIDKLKDRIPRVKMAGIEY
                     GPDSSFDIDPQHWPRVGSQT"
     gene            1227..2450
                     /locus_tag="VF276_16310"
     CDS             1227..2450
                     /locus_tag="VF276_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEX6608466.1"
                     /translation="MTQTATPTTPVLPNPPKRLLLGPGPSEVDPTVLQALALPPLGHL
                     DPALLQLMADLQNQLRAVFQTHNHVTLAVSGTGSAGMEAALLNTVAPGDTVLVGVMGY
                     FGERLCAVAERLGGKIHRVEGEWGRPLDPQRLADEIRRIRPQVVALVHAETSTGVLQP
                     LDGIGAATQAVDALLVLDTVTSLGGHPVQVDAWGVDACYSGSQKCLGAPSGLAPFTIN
                     DRALARRRGRATPPAGYYLDANLLAAYWDDHQYHHTISAPLIYALYTALRLLETEGME
                     RRWARHQLQHRAFRAGCEALGLRFLPDESYSLWPLNAVCVPEGLDDMRARAALRDHHN
                     IEIGGGIGPLKGKLWRIGLMGYGARQEFVLELLGALELMLKTHGHGATPGAGVAAAMG
                     VYAQEAAQGDSGAIG"
     gene            complement(2607..>3666)
                     /locus_tag="VF276_16315"
     CDS             complement(2607..>3666)
                     /locus_tag="VF276_16315"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEX6608467.1"
                     /translation="ALPGLTLSTGRLPAAESLLRTFARYIDQGMVPNLFPDEGTAPDY
                     NTVDATLWYFHAINAYLEAGGNMALASDLWPQLEDIIRWHVKGTRYNIGVDPTDGLLH
                     AGEPGVQLTWMDAKVDDTVFTPRHGKPVEIQALWYHALRIMARLQGQVAAAAATAGPA
                     TDGAGEAPAPLDYTAMAARARDSFRARFWYAEGGYLYDVVDDPNGGDDTSLRPNQLFA
                     LSLEPDLVDETQARSVLDVVRRELLTPVGLRTLAPSDPRFQAVYTGNRATRDAMYHQG
                     PVWPWLIGAYGDAVRRWEGVDRAGIRKLLEPLQRQLAVDGTGTINEIFDAQPPYNPRG
                     CFAQAWSVSEVLRLLRTP"
BASE COUNT          565 a         1242 c         1264 g          595 t
ORIGIN      
        1 atcaatggct acaactgcgg tggcgcgggc gagccgtgcc ccggtctcta ctaccggcct
       61 ttcaacaaca tcacccgcgg ccaggtgagc aaggtggcct cccagacgtt cttcccgaac
      121 gcctgtgcgc ccgctgcgcc ggctgccggc aagaacccgt aacccccggt ccagggacgg
      181 cgccctgtgt gccgcccctg gacccccacc cgcgcgcccg gccccgcgcc gggcgcgcgg
      241 cacccgcggc accacgggcg ccggcgatcc ctgatcacat ggtgcccgcc caccgcgccg
      301 ccggaccaac cggcggcggc gccaacggca acggccggac ccgagggtcc ggccgtttgc
      361 ttgttaccgc gtgtcgcctg agatcaggtc tggctgccga cgcgcggcca gtgttgcgga
      421 tcgatatcga acgagctgtc gggtccgtat tcgatcccgg ccatcttcac gcgcgggatg
      481 cggtctttga gcttatcgat gttcacgagc aagaaggtgc ccatctgatg gatcagcagg
      541 cgcacggagc cgaagatcgt ctctaactgg gtatggtact tcggcgaggt gaactgcttg
      601 agcacctcca gatacatatt gaccgagcgc tcgatttccc gcgtgcgcca gttgatcaag
      661 cggggcgtga gcgggttccc ggcgtcgatg tcgagatagt agcgctgcat gaagatgata
      721 atatgatgct ggcggaacag ggccacatcc tgcgtctgca cataatcggt gatgacctcc
      781 agcacgttcg cgtagcgcgc gcggaggatt ttctgcttgt tcatttcgtc gtacagggtg
      841 cccttcaact cctgcggagc gcggtgcatc aattcatcga gtatcgcggt ggcatgttcg
      901 cgaagttcat cggccagtgc ggccataagt tgatcccctt ccttctgagg tccgccgccc
      961 ggtcgccggg acaccggagc gcgaatccgc gtcgtctaag catagcagaa agcccccgcg
     1021 cccgcaaccg cgatccccgg cgcacctgca cgagcgaata aaataggccg cgcaggcggc
     1081 ctttgttcgc gtagccggag gcttgagcct ccgggcgccc gcgggcggcg gcaccctttc
     1141 tgctgtgccg tccctgttcg acggcgtgag tgccgggggc gccgcgtgcg ccgcactccg
     1201 cccaccctgc tattggagga tcccgcatga cacaaaccgc aacccccacg acccccgtgc
     1261 tgcccaaccc gccgaagcgc ttgctgctcg gtcccggccc cagcgaggtc gatcccaccg
     1321 tgttgcaggc gctggcgttg ccgccgctgg gccatctcga cccggcgctg ctgcaactga
     1381 tggccgatct gcaaaaccag ttgcgcgccg tctttcagac ccacaaccat gtcacgctgg
     1441 cggtatcagg cacgggcagc gcgggcatgg aagccgcgct gctcaacacc gtcgcccccg
     1501 gcgatacggt cctggtcggc gtgatgggct atttcggcga gcgcctctgc gcggtcgccg
     1561 agcgcctggg cggcaaaatc caccgggtgg aaggtgaatg gggtcgcccg ctcgacccgc
     1621 agaggctggc cgatgagatc cgccggatcc gaccccaggt cgtcgcgctg gtccacgccg
     1681 aaacctcgac cggcgtgctc cagcccctcg acggcatcgg cgcggccacg caagcggtcg
     1741 acgcgctgct ggtgctcgac acggtgactt cgctcggcgg gcacccggtg caggtggacg
     1801 cgtggggggt ggacgcctgc tatagcgggt cgcagaagtg cctgggcgcg ccctccggcc
     1861 tggcgccctt caccatcaac gacagggcgc tggcccgccg gcgcgggcgc gccactccgc
     1921 cggcgggcta ttacctggac gccaacttgc tcgcggccta ttgggatgac caccagtatc
     1981 accacaccat ctcggcgccg ctgatctatg cgctctatac ggcgctgcgc ttgctggaga
     2041 ccgagggaat ggaacggcgc tgggcgcggc accagttaca gcaccgcgcc ttccgcgccg
     2101 ggtgcgaggc cctgggcctg cgcttcctgc ccgacgaatc gtacagcctg tggccgctga
     2161 atgcggtgtg tgtgcccgag gggctggacg acatgcgggc gcgcgccgcg ctgcgcgacc
     2221 accacaacat cgagatcggc ggcggcatcg ggccgctgaa gggcaagctc tggcgcatcg
     2281 ggctgatggg ctacggcgcc cgccaggagt ttgtgctcga actgctgggc gccctggaac
     2341 tgatgctcaa gacccacggc cacggcgcca caccgggggc cggcgtggcc gcggccatgg
     2401 gggtgtacgc gcaggaggcc gcccagggcg attccggcgc catcgggtag gcggtgggcc
     2461 ggtttcaaac cggcccctac agcgcaaagg accggggcgg gccggtttga aaccggcccc
     2521 tacagcacag agggacgccg tacccgaata aggcgcccgt gccgggcagc cggtacgggc
     2581 gctttgtatg cctgccgggc gcccgattac ggggtccgca gcaggcgcag cacctctgag
     2641 acgctccagg cctgggcaaa gcagcccctg gggttatacg gtggctgggc gtcgaagatt
     2701 tcgttgatcg tgcccgtgcc gtccacggcc agttggcgct gcagcggctc cagcaacttg
     2761 cggatgcccg cgcggtccac gccctcccag cgccgcacgg cgtcgccgta ggcgccgatc
     2821 agccagggcc agacgggtcc ctgatggtac atggcgtcgc gggtagcccg gttcccggtg
     2881 tagacggcct ggaaccgggg atcgctgggc gccagggtgc gcagccccac cggtgtcagg
     2941 agttcgcggc gcaccacgtc gagcacgctg cgggcctggg tttcgtccac caggtcgggt
     3001 tcgagcgaca gagcgaacag ttggttgggg cgcaggctgg tatcgtcgcc gccgttggga
     3061 tcgtcgacca cgtcgtacag atacccgccc tcggcgtacc agaaccgggc gcggaagctg
     3121 tcccgtgccc tggcggccat agccgtgtag tcgagtggcg cgggcgcttc ccccgcgccg
     3181 tcggtggcgg ggccggcggt ggccgccgca gcggccacct gaccctgcaa gcgggccatg
     3241 atgcgcaagg cgtggtacca gagggcctgg atctcgaccg gcttgccgtg gcgcggggta
     3301 aagaccgtgt cgtcgacttt agcgtccatc caggtaagct gcacgcccgg ttcgcccgcg
     3361 tgcaacaggc cgtcggtggg gtcgacgccg atgttatagc gggtgccctt gacatgccag
     3421 cggatgatgt cctccagttg cggccagagg tcgctcgcca gggccatgtt gcccccggct
     3481 tccagatagg cattaatggc gtggaagtac cagagagtgg cgtccaccgt gttgtaatcg
     3541 ggcgcggtgc cttcgtcggg gaagaggttg ggcaccatgc cctgatcgat atagcgggcg
     3601 aaggtgcgta gcaagctctc ggcggcgggt agccggcccg tgctcagcgt caggccaggc
     3661 aaggcg
//
LOCUS       DASUYC010000999         2076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_166864,
            whole genome shotgun sequence.
ACCESSION   DASUYC010000999 DASUYC010000000
VERSION     DASUYC010000999.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166864"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..988
                     /locus_tag="VF276_16320"
     CDS             185..988
                     /locus_tag="VF276_16320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608468.1"
                     /translation="MTIASRPAGMLVGLFLAVLVVSAIVNLLPAEWQESPFTVPAIAL
                     WRGGLAPRDGTATAARTPQAGISTDRPDLPPRDTGRRGFGPFGISRASVGGFTLHLTS
                     LITLLCGGLLILYLLPARIGRIAAALRGSWPARVGLGLLGLAAAVILAALGLVIGVAL
                     GGASLITFLGVPIWLAITAIGPVAALLGLTAVSLPLGRWTGRRLGLAEQPPQVDLLAG
                     LLVLFLVGLVPVLGGLVLLCAALLGLGAVLQTRAGSARPWAFRLPDLEY"
     gene            1085..>2076
                     /locus_tag="VF276_16325"
     CDS             1085..>2076
                     /locus_tag="VF276_16325"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEX6608469.1"
                     /translation="MGKLLSKKVLFAIGALVVVVALVLYGVSAFVGRNARAATNLSTV
                     TVQRGDLQATVLSSGALQPAADVTLSFGSGGTVADIFVKNGDVVTKGQKLAALDTRDL
                     QFAVDQAQANLTSAQAKYDQVKAGAAPKDIANAQSQLAAAQAKLDSLKAGPTKEDLAT
                     AQAQVTSAQAKLHALSVGPSAQDVANAQSQITAAQAKLDALKAGPTAEDVANAQSQIA
                     SAQAKLDALKAGPSPEDVASAKSQITSAQAKLDALTAGPTTAELSSAQLKVISAQTSF
                     DKVSSSASLAKQNAEKALDQAGNAVRNAQDLLNEAAVINVPRQGQQNIVNPDGS"
BASE COUNT          303 a          688 c          742 g          343 t
ORIGIN      
        1 gtcggcacgc tgcccggcgc cggcacctcc gccgaagcct atctcgggct ggtcgtcggg
       61 gcgctgatcc tggcggtggg ggcgggcgtg ttcctgctgc acgcgctcaa tacatcggac
      121 tcctccacgg ggacggtccc cgcggggcgt ttgcaactac gctagctgac aaggtggaag
      181 cccggtgact atcgcttcgc gcccagccgg tatgctggtt gggctgtttc ttgcggtcct
      241 ggtggtctcg gcgatcgtga acctgctccc cgccgaatgg caggagagtc cgttcaccgt
      301 gcccgcgatc gcgctgtggc ggggcgggct cgccccgcgc gacggcaccg ccacggcggc
      361 ccgcaccccg caggccggca tcagcaccga ccggcccgac ctgcccccgc gcgacacggg
      421 gcggcggggc ttcggtccct tcggcatcag ccgggccagt gtgggcggct tcacgctgca
      481 cctgacctcg ttaatcacgc tgctctgcgg cgggttgctg atcctgtacc tcctgccggc
      541 acgcatcggc cgcatcgcgg cggcgctgcg cgggtcgtgg ccggcgcggg tgggtctggg
      601 cttgctcggg ctggctgccg ccgtgatcct ggccgcgctc ggcctggtga tcggggtcgc
      661 cctgggcggt gcctcgctga tcaccttcct gggcgtgccg atctggctgg cgatcacggc
      721 tatcgggcct gtggcggcgc tgctgggcct cacggcggtg agcctgccgc tggggcgctg
      781 gaccgggcgg cggttgggcc tggccgaaca gccgccgcag gtcgatctgc tggccgggct
      841 gcttgtgcta ttcctggtcg gtctggtgcc cgtgctgggc gggctggttt tgttgtgcgc
      901 ggctctgctg ggcctgggcg cggtgttgca aacccgcgcc ggcagtgccc ggccctgggc
      961 attccggctg cccgatctgg agtactaacc gccggatggt ccgcggttcc cgttgtcctt
     1021 tgccccttgc aaatcacgct gggaaccggc atgaactggc ataagggatg ggagagacgt
     1081 gtcaatgggt aagttgttaa gcaagaaagt gctgttcgcc atcggcgcgc tggtcgtcgt
     1141 cgtggcgctg gtactatatg gggtgagtgc gttcgtgggg cgcaacgccc gcgcggcgac
     1201 gaatttgagc accgtcactg tgcagcgcgg cgatttgcag gccaccgttt tgtccagcgg
     1261 ggccttgcaa cccgccgccg acgtgacgct cagcttcggc agcggcggca cggtggccga
     1321 catttttgtg aagaacggcg atgtcgtcac caagggccag aaactggccg ccctggatac
     1381 ccgcgatctg cagttcgcgg tcgaccaggc gcaggcgaac ctgacctcgg cccaggccaa
     1441 gtatgaccag gtgaaggccg gggcggcgcc gaaggacatt gccaacgcgc agtcgcaact
     1501 ggcggcggcg caggccaagc tcgactcgct gaaggccggg ccgaccaagg aggatctggc
     1561 gaccgcgcag gcccaggtca cttcggccca ggccaagcta cacgcgctga gcgtcgggcc
     1621 gagcgcccag gacgtggcca atgcccagtc gcagattacg gcggcgcagg cgaagctcga
     1681 cgcactgaag gccgggccga cggcggaaga tgtcgccaac gcccagtcgc agattgccag
     1741 cgcccaggcc aagctcgacg cgctgaaggc cgggccgagc cccgaggacg tggcgagcgc
     1801 caagtcgcag atcaccagcg cccaggccaa gcttgacgcg ctgaccgcgg gccccaccac
     1861 cgccgaactg agttcggcgc aactcaaggt gatctcggcc cagaccagct tcgacaaggt
     1921 tagctcgtcg gcctcgctag ccaagcagaa cgcggagaag gctctggatc aggcgggcaa
     1981 cgcggtgcgt aatgcccagg atctgctcaa cgaagcggcc gtcattaatg tgccgcgcca
     2041 gggccagcag aatatcgtca atcccgacgg cagctt
//
LOCUS       DASUYC010001000         4695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1105001, whole genome shotgun sequence.
ACCESSION   DASUYC010001000 DASUYC010000000
VERSION     DASUYC010001000.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1105001"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="VF276_16330"
     CDS             complement(<1..774)
                     /locus_tag="VF276_16330"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX6608470.1"
                     /translation="MSRDFRDVNTVTAGVFTAEHSATRNWLLSAIFWLTIADFIGLIG
                     AIEMMSPDAFAGIPWLEFGRVRQMHTNGVLFLWLSMAQIGAFHYIVPKLCGTRLHSEL
                     LGNILMVVWNFVGILAIATLSNGLTQGREYAELIWPIDVLVMVGLLGTAYNLFRTILA
                     RKEKKLFVSLWYIMGTMIWMPTLYFVGNVMWEPIFNFGTSNLAGFPSGSLQGIDDVIW
                     QWFYGHNVLGYWFTTSGVAITYYVVPVIIRSPLYSHLLSL"
     gene            complement(797..1663)
                     /locus_tag="VF276_16335"
     CDS             complement(797..1663)
                     /locus_tag="VF276_16335"
                     /inference="COORDINATES: protein motif:HMM:NF021010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HEX6608471.1"
                     /translation="MEWTRFTQPLQTNPRLRAAALLAVLLAAGALFSLANVSFVRAAT
                     ASITVASINSPVPAADPFSPLWQQSAPADVPLSAQQMWQPGGGSVSAVQVRALEDGQN
                     IAFLVSWADATRNDYVRDMPSDAGAIQLPTNPASLPYQCMGQSTNVVNIWQWKAAIEK
                     TAHTSGQPYNGTRNLTSNGMGKAVETRGVPPTATSTWRDGHWYVVYARKLAPAGPGTA
                     PLMLGENTNAAFAIWDGAKGETRSMKAVSTWTPVELPGTTTNEAMNLVSLAILGLVAA
                     GAVVLAWRIIPH"
     gene            complement(1685..2194)
                     /locus_tag="VF276_16340"
     CDS             complement(1685..2194)
                     /locus_tag="VF276_16340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608472.1"
                     /translation="MQMQPGPPAAPQPPGTGTPAPGPGIVAPITLVFEHPLHPAQRLV
                     IDRVRTLMQVHRFELHNIQPSGESIHHIIAADHIRNIELLYDDATPDRLVAQFTVNSK
                     EDKEALVWTSSLFFQYLHYHVARIATVNGAPHQMDWEAQQDPQGPERVKRWVEQGHSL
                     IERMHVTER"
     gene            complement(2200..3075)
                     /locus_tag="VF276_16345"
     CDS             complement(2200..3075)
                     /locus_tag="VF276_16345"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX6608473.1"
                     /translation="MRDKVILGATLTVVLLLTLLVYAAVDFQRTPAAQASAREQAVAA
                     GTRIYAQYCVQCHGPKGEGCIGPALNRNVWRPEINGAANPNYQPDDIPTLVYKTIERG
                     RKSNQPGQQMPAWSVAEGGSLNDQEINQVIAFIQYGDWDQVVEHAASATGLDKDLPDY
                     SSKGFNGGQADPAKLAQVKSTMLAKGCLNCHQVGAVGGKIGADLTDVGSRRTADWLRR
                     WIPNPSAMPPLERGPNLWLNVAVPSLETPGPGVPVAPTPQPFPMNATFMPTIKMTDAE
                     LDLLVDYLSHARTGQ"
     gene            complement(3091..3684)
                     /locus_tag="VF276_16350"
     CDS             complement(3091..3684)
                     /locus_tag="VF276_16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEX6608474.1"
                     /translation="MSQQVRTERTLTPRVHTRPGPEDTQGGLVQGASRRDFLRLTMLG
                     SLGVMSLGGIGAFLAYFWPRKIGTFGSKISAGTLDDIKDGDVVMFREGKFYLSRYQEP
                     ALGNKDVIIALYWKCKHLGCTVPWKPDESFGANQGIFHCPCHGSIYLRTGQNVAGPAP
                     APLDIMEITLDGRKVTVNTGKITTRVQYEPKQATPVS"
     gene            complement(3721..4587)
                     /locus_tag="VF276_16355"
     CDS             complement(3721..4587)
                     /locus_tag="VF276_16355"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinol-cytochrome C reductase"
                     /protein_id="HEX6608475.1"
                     /translation="MATESRPAPGVTIRERNVTEQKGAPVLTASPRTRPVSPAAVEAD
                     TVQVWPHLVVIEFLGAVVFTINLILLSTLVNSPLEELANGDVTPNPSKAPWYFLNLQE
                     LLLHMNSALAGVIVPTVALIGLAAIPYIDKGTRGLGVWFYNERGPRIAAFSMIYTTVV
                     LAFLIGFDVPQGRLKDALSTSLKPGGFLDWMPGAFSFLGDVPTAHGILVEGIAGWIIP
                     TIIMIGFMILLVVLLKLMFKGLTTSELVIGLFSAFVATFVILTFVGTFMRGGGMTLYM
                     PWDPRASGVVGH"
BASE COUNT          807 a         1491 c         1583 g          814 t
ORIGIN      
        1 caacgacagc aggtggctgt agagcggcga ccggataatc accggcacga catagtaggt
       61 gatggcgacg ccgctggtgg tgaaccagta gccgagcacg ttgtggccgt agaaccactg
      121 ccagatcacg tcgtcgatgc cttgcaggct gcccgagggg aacccggcca ggttgctggt
      181 gccgaagttg aaaatgggtt cccacatcac gttgccgacg aagtagaggg tcggcatcca
      241 gatcatggtg cccatgatat accagaggct gacgaagagc tttttttcct tgcgggccag
      301 aatggtgcgg aacaggttgt aggcggtgcc gagcaggccc accatcacca gcacgtcgat
      361 gggccagatc agttcggcgt actcgcgacc ctgggtcagc ccattggaga gggtggcaat
      421 tgccaggatg ccgacgaagt tccacaccac catgaggatg ttgcctaaca gttcgctgtg
      481 caggcgggtg ccgcagagct tgggcacgat atagtggaag gcgccgatct gggccatgct
      541 gagccagagg aagagcacgc cgttggtgtg catctggcgg acgcggccaa actccagcca
      601 ggggattccg gcgaaggcgt cggggctcat catctcgatg gcgccgatca gcccgatgaa
      661 gtcggcgatg gtcagccaga aaatggccga caacaaccag ttgcgggttg cgctatgttc
      721 agcggtgaac acgcccgccg tcacggtatt cacatcgcga aaatctctgc tcatgcgcca
      781 agctcctcct gaggccctag tgcgggatga tgcgccaggc cagaacgacg gcccccgcgg
      841 cgacaagtcc gagaatggcc agcgagacca ggttcatggc ctcgttggtg gtggtgccgg
      901 gcagttccac cggagtccag gtcgagacgg ctttcatgct gcgggtctcc cccttggcgc
      961 cgtcccagat cgcgaaggcg gcattggtgt tctcgccgag catcagcggg gccgtgccgg
     1021 gaccggcggg cgccagcttg cgcgcgtaga cgacgtacca gtggccgtcg cgccaggtgc
     1081 tggtggcggt cggcggcacc ccgcgcgttt ccaccgcctt gcccatgccg ttgctggtca
     1141 ggttgcgcgt gccgttgtag ggctgcccgc tggtgtgcgc cgtcttctcg atggcggcct
     1201 tccactgcca gatgttgacc acgttggtgc tctggcccat gcactgatac ggcaggctcg
     1261 ccggattggt cggcaactgg atcgcgcccg cgtcgctggg catatcccgc acatagtcgt
     1321 tgcgcgtggc gtcggcccac gaaaccagga aggcgatatt ctgcccatct tccagggcgc
     1381 gcacctgcac cgcggagacg ctgccgccgc cgggctgcca catctgctgc gcgctcagcg
     1441 gcacatcggc gggcgccgac tgctgccaga gcggcgagaa cgggtcggcg gccggcacgg
     1501 gcgagttgat cgaggccacg gtaatcgagg cggtggccgc gcgcacgaag ctgacgttcg
     1561 ccaggctaaa caaggcgccg gcggccagca agaccgcgag caacgcggcg gcccgcaagc
     1621 gcggattcgt ctgcaacggc tgcgtgaacc tggtccattc catacagtcg taccctctac
     1681 gtgattagcg ttcggtcacg tgcatacgct cgatgagcga atgcccctgc tcgacccagc
     1741 gcttcacgcg ctcggggcct tgcgggtctt gctgggcttc ccagtccatc tgatgcggcg
     1801 ccccattgac ggtggcaatg cgggccacat ggtaatgcag gtattggaaa aacagcgacg
     1861 aagtccagac cagggcttct ttgtcctctt tggagttgac cgtgaactgg gccaccagcc
     1921 ggtcgggcgt cgcgtcgtcg tagagcaatt cgatattgcg gatatgatcg gcggcgatga
     1981 tatggtggat cgattcgccg ctgggctgga tgttgtgcaa ctcgaaccga tgcacctgca
     2041 tgagcgtgcg cacccggtcg atcaccaggc gttgcgccgg gtgcagcggg tgttcaaaca
     2101 ccagggtgat cggggcgacg atgccggggc cgggcgccgg agttccggtg cccggcggct
     2161 gcggcgccgc cggcgggcca ggctgcatct gcaccggccc tactgccccg tgcgcgcgtg
     2221 cgacaggtag tccaccagca aatccaactc cgcgtcggtc atcttgatgg tcggcatgaa
     2281 ggtcgcattc atcgggaaag gctgcggcgt gggcgccacg ggcacgccgg gaccgggtgt
     2341 ctccaggctg ggcaccgcca cgttcagcca caggttcggc ccgcgttcca gcggcggcat
     2401 ggcgctcgga ttcgggatcc agcggcgcag ccaatcggcg gtgcggcgcg accccacgtc
     2461 ggtcaggtcg gcgccgatct tgccgccgac ggcgcccacc tggtggcagt tgaggcagcc
     2521 cttcgccagc atggtcgact tcacctgggc cagcttggcc gggtcggcct ggccgccgtt
     2581 gaagcccttg ctgctgtagt cgggcaggtc cttgtccagg ccggtggccg acgccgcgtg
     2641 ctccaccacc tggtcccagt cgccatactg gatgaaggcg atgacctggt taatctcctg
     2701 gtcgttgagc gatccgccct cggccaccga ccaggccggc atctgctggc cgggctggtt
     2761 cgacttgcgc ccgcgctcga tcgtcttata gacgagcgtg gggatgtcgt cgggctggta
     2821 gttggggttg gccgcgccgt tgatctccgg gcgccagacg ttgcggttca gcgccgggcc
     2881 gatgcagccc tcgcccttgg ggccgtggca ctgcacgcaa tattgagcat agatgcgcgt
     2941 gcccgcggcg accgcttgct cacgcgcgct ggcctgggcc gcgggggtgc gctggaaatc
     3001 gaccgccgca tacaccagca gcgtcagcag caacacgacc gtcagggtgg cccccaggat
     3061 cactttatct cgcatagcgg tgtcacactc ctaagaaact ggtgtggctt gcttcggctc
     3121 gtactgcaca cgggtcgtga tcttgccggt gttcacggtg accttccgtc cgtccagggt
     3181 gatctccatg atgtcgagcg gcgccggggc cggcccggcc acgttctgcc cggtccgcag
     3241 gtagatcgag ccatggcacg ggcaatggaa gatcccctgg ttggccccga acgactcatc
     3301 gggcttccag ggcaccgtgc agcccaggtg cttgcacttc cagtagaggg cgatgatcac
     3361 atccttgttg cccagcgccg gctcctggta gcgcgagaga tagaatttgc cctcgcggaa
     3421 catcaccacg tcgccgtcct tgatatcgtc gagcgtgccg gcgctgattt tgctgccgaa
     3481 cgtgccgatc ttgcgcggcc agaaataggc caggaaggcg ccgatcccgc ccaggctcat
     3541 cacgccgagc gaccccagca tggtcagacg cagaaaatcg cgccggctgg ccccctggac
     3601 caggccgccc tgggtatcct cgggaccggg ccgggtatgc acgcgcggcg tcagggtccg
     3661 ctcggtccgt acttgctgcg acattacttt cctcctctaa tgccaaccgg gccggcgtgc
     3721 ttagtgcccg acaacgcccg acgcccgcgg gtcccagggc atgtacaggg tcatgccgcc
     3781 gccgcgcata aaggtgccga cgaaggtgag tatcacaaag gtggcgacga acgcgctgaa
     3841 cagaccgatc accagctcac tggtggtcag gcccttgaac atcaacttca agagcaccac
     3901 caggaggatc atgaagccga tcataatgat cgtcgggata atccagccgg cgatgccttc
     3961 caccaggata ccgtgcgcgg ttgggacatc gcccaggaag ctgaacgcac ccggcatcca
     4021 gtccaggaac ccgcccggct tgagcgaggt ggacagggcg tccttgagcc ggccctgcgg
     4081 cacgtcgaag ccgatgagga aggccaggac gacggtggtg tagatcatgg agaacgccgc
     4141 gatgcgcggc ccgcgctcgt tatagaacca gacccccagc ccgcgcgtgc ccttgtcgat
     4201 gtacgggatc gccgccaggc cgatcagggc cacggtcggc acaatcacgc cggccagcgc
     4261 cgagttcatg tgcagcagca gttcctgcaa gttcaggaag taccagggcg ccttcgacgg
     4321 gttcggcgta acgtcgccgt tcgccagctc ttccagcggt gagttgacca gcgtggagag
     4381 caagatcagg ttaatggtga agaccaccgc gcccaggaac tcgatgacga ccaggtgggg
     4441 ccacacctgc acggtgtcgg cctccacggc ggcgggcgag acgggccgcg tgcggggcga
     4501 ggcggtcagc accggcgcgc ccttctgctc ggtcacgttc cgttcgcgaa tggtcacccc
     4561 cggcgcggga cgactttcgg tagccataac agggctcctt ctaaataagg gggcgaatga
     4621 tcactcgcgg gcgcgcccgg cggaccgggc cccggcggac aggttctcgc cgggatcggt
     4681 gatgcgcgtc gtgaa
//
LOCUS       DASUYC010001001         3018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1890137, whole genome shotgun sequence.
ACCESSION   DASUYC010001001 DASUYC010000000
VERSION     DASUYC010001001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1890137"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VF276_16360"
     CDS             complement(<1..292)
                     /locus_tag="VF276_16360"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6608476.1"
                     /translation="MSTEVWASALAYEPYMGRWSRLVAAEFLAWLDVPPGGRWIDLGC
                     GTGALSGTILDRADPFDLTAIDRSAGYTQYARRQVRDDRAHFLVGDAMTLPLA"
     gene            complement(418..1713)
                     /locus_tag="VF276_16365"
     CDS             complement(418..1713)
                     /locus_tag="VF276_16365"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEX6608477.1"
                     /translation="MPDLTLGPRAEDFIWASGIEDTFVPQTRTGHRALDEYQLMGHYD
                     HWREDLALARDLGVQALRWGVPWYRVEPLPGEFDWSWTDQVIPYLVQELGITPIIDLM
                     HYGCPFWLRREFISDEYPQAVAAYAGAFARRYGHLVRWYTPLNEPQVNAMMCGQRGLW
                     PPYLRGTTGFVRVMLQLVKGIILTVETLKAIRSDAMMVHVDATGLSRAAVPELEALAQ
                     EDQLRGYLCYDLLTGRVTPEHPLFPWLVRNGASVTELAEIAARPLTLDVLGINFYPQW
                     STRQLHLDAQGRLAYRITEQAGTGFGALILDYYKRYQAPIIVTETSAHGPNPRRERWL
                     DAALAAIKALRRHGVPVLGYTWFPLFTMIDWRYRFARGPVEEYRIELGLYHLGDPDSD
                     RRWIPSPLTERFRQYIQHPEEAVGPLLAAATQPAAAHGS"
     gene            complement(1734..>3018)
                     /locus_tag="VF276_16370"
     CDS             complement(1734..>3018)
                     /locus_tag="VF276_16370"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEX6608478.1"
                     /translation="GAGVSTDFQWMTGFECSAFPQYGADELAETQHYRWWASDIERLR
                     DVGITMIRYGIPWHRVNPQPHVYDWGWTDQALDLLETLGITPIVDMFHYGTPLWIEGG
                     IMNPIFGEMQSWYAKTFAERYPHITYYTPTNEPYICATFGAEWAIWYPFLRGDGNAVR
                     AIKNVSEGVARSMAEIRRVRPDALMMIPDTCEYYHSVDGAFQEEADFRTERRFLVHDL
                     YQGLVTREHPMWDYMVSNGIHEWELEWFLRNPVRLDILGLDYYQHSEHQLRRGLNGER
                     IDETATQQHGWAEMARQYSTRYGGIPVILAETNVGGPVEDRVRWVEQLVAQTRQARAA
                     GTPVFGLTYYGAIDHVDWDTALRVRNLNINPCGMWALHWEGDRLVRTPTALVDLFRRY
                     IAMPTADSVGPLASEDAAVRVRAVLAPLGRTRRGA"
BASE COUNT          483 a         1006 c         1040 g          489 t
ORIGIN      
        1 gcgccagcgg cagcgtcatg gcgtcgccga ccaggaagtg ggcgcggtcg tcgcggacct
       61 gccggcgcgc gtactgcgtg tagcccgcgg accggtcgat agccgtgagg tcaaacgggt
      121 cggcgcggtc caggatggtg ccgctgagcg cgccggtgcc gcaacccagg tcgatccagc
      181 gcccgcccgg cgggacgtcc agccaggcca ggaactcggc ggccaccaac cggctccacc
      241 ggcccatata gggctcatag gccagcgcac tggcccagac ttcggtgctc atcgtcactc
      301 cctcaccggc ggtcaggccg ccgggcacct gccccgtcat tcacagccat aatgcaccga
      361 tcaggcggtc tcctgctcgt cacacgccgc ccttgcagac accgctgtga gaataatcta
      421 cgatccgtgc gctgccgcgg gttgcgtcgc cgctgcgagt agcgggccga cggcttcttc
      481 ggggtgctgg atgtactggc ggaagcgctc ggtcagcggc gaggggatcc agcgccggtc
      541 gctgtcgggg tcacccaggt ggtacaggcc gagttcgata cggtattcct ccaccggccc
      601 gcgcgcgaag cgatagcgcc agtcgatcat cgtaaacagc ggaaaccacg tatagcccag
      661 cacaggcacg ccgtggcggc gcagcgcctt gatcgcggcc agggctgcat cgagccagcg
      721 ctcgcgccgc gggttcgggc cgtgggcgct cgtctcggtg acgatgatcg gcgcttggta
      781 gcgcttgtag tagtcgagga tcagtgcgcc gaagccggtg cccgcctgtt cggtgatgcg
      841 gtaggccagg cggccctgcg cgtccaggtg caactggcgc gtggaccact gcggatagaa
      901 gttgatgccc agcacgtcga gcgtgagcgg gcgcgcggca atctccgcca gttccgtgac
      961 cgacgcgcca ttacgcacca gccaggggaa cagcgggtgc tcgggcgtga cgcgcccggt
     1021 cagcagatcg tagcagagat agccgcgcag ttggtcctcc tgggccagcg cctccagttc
     1081 gggcacggcg gcgcggctca agccggtggc gtccacgtgg accatcatcg catcggaccg
     1141 gatggccttg agcgtctcca cggtgaggat aatgcccttg accagttgca gcatgacccg
     1201 cacaaagccg gtggtgccgc gcaaataggg cggccacagc ccgcgctggc cgcacatcat
     1261 cgcgttcacc tggggctcgt tgagcggcgt gtaccagcgc accaggtgac cgtagcgccg
     1321 ggcaaacgcg cccgcatagg cggcgacggc ctgcggatat tcgtcgctga tgaactcgcg
     1381 ccgcagccag aaggggcagc cgtagtgcat caggtcgatg atcggcgtga tgcccaattc
     1441 ctgcaccagg taggggatca cctggtcggt ccaactccag tcgaactcgc cgggcagcgg
     1501 ctcgacccga taccagggca cgccccagcg caacgcctgc acgcccaggt cgcgagccag
     1561 ggccaggtcc tcgcgccagt ggtcgtagtg gcccatcaac tggtattcgt ccagcgcgcg
     1621 gtggccggtc cgcgtttgcg gcacaaaggt gtcttcgatc ccgctggccc agatgaaatc
     1681 ttcggcccgc ggccccaggg tgagatcggg cacagccccc tccgttggtg gattcatgcg
     1741 ccgcgccggg tgcggcccag cggcgccagc accgcccgca cgcggacggc ggcatcctcg
     1801 gacgccagcg gccccacgct gtccgcggtg ggcatggcga tatagcggcg gaacaggtcg
     1861 accagcgcgg tgggggtgcg gaccagccgg tcgccctccc agtgcagcgc ccacatgccg
     1921 cagggattga tgttgaggtt gcgcacgcgc agcgccgtat cccaatcgac gtggtcgatg
     1981 gcgccgtagt aggtgagccc gaagacgggc gtgccggcgg cgcgcgcctg gcgagtctgc
     2041 gccaccagtt gctccaccca gcgcacgcgg tcttccaccg gcccgcccac gttagtttcg
     2101 gccaggatca ccgggatgcc gccgtagcgc gtggaatact ggcgcgccat ctccgcccag
     2161 ccgtgctgct gcgtcgccgt ctcgtcgatg cgctcgccgt tgaggccgcg ccgcaattgg
     2221 tgctcggagt gctggtagta gtcgagcccg aggatatcga ggcgcaccgg gttgcgcagg
     2281 aaccactcca gttcccactc gtggatgccg ttgctgacca tgtagtccca catcggatgc
     2341 tcgcgcgtga ccaggccctg gtagagatcg tgcaccagga agcggcgctc ggtacggaaa
     2401 tcggcctcct cctggaaggc gccgtccacc gagtgatagt actcgcaggt gtcagggatc
     2461 atcatcagcg cgtcggggcg cacgcggcgg atctcggcca tcgagcgggc cacgccctcg
     2521 ctgacgttct tgatcgcccg cacggcgttg ccgtcgccgc gcaggaaggg gtaccagatg
     2581 gcccactcgg cgccgaaggt cgcgcagata tacggctcgt tggtgggcgt gtagtaggtg
     2641 atatgcggat agcgctcggc gaaggtcttg gcgtaccagc tttgcatctc gccgaagatc
     2701 ggattcatga tgccgccctc gatccagagc ggcgtgccgt agtggaacat gtccacgatg
     2761 ggcgtgatgc ccagcgtctc cagcagatcg agcgcctggt cggtccagcc ccagtcgtag
     2821 acgtgcggct gggggttgac acggtgccag gggatgccgt agcggatcat ggtgatgccc
     2881 acgtcgcgca ggcgttcgat atcgctggcc caccagcggt agtgctgcgt ctcggccagt
     2941 tcgtccgcgc cgtactgcgg gaaggccgag cattcaaacc cggtcatcca ttggaagtct
     3001 gtgctcacac ctgctcct
//
LOCUS       DASUYC010001002         2355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_399615,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001002 DASUYC010000000
VERSION     DASUYC010001002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399615"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="VF276_16375"
     CDS             <1..446
                     /locus_tag="VF276_16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX6608479.1"
                     /translation="DVEPRTHLGDLLRDELQLTATHLGCEHGVCGACTVLLDGVPVRS
                     CLTFAVQAGGQEVGTVEGLAADPEHLHPLQQGFWEAHGLQCGFCTPGFLMTLLPFLVE
                     HPDPSETEIREALSGNLCRCTGYQNIVAAVQLAARKLRAPAGDAS"
     gene            443..>2355
                     /locus_tag="VF276_16380"
     CDS             443..>2355
                     /locus_tag="VF276_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6608480.1"
                     /translation="MSTQMFGARIKRSEDPQLLTGRAQFVDDVHIPGMVHAAFKRSDY
                     AHARITGIDVEAARALPGVLAVYTAADLGDYLQPSPLLVQPPPTQHIVFNQRTATQLA
                     QDKVRYVGEPIAMVVAESRYLAEDAAEQIVVDYEPLPAVVDLEAAMAPAAPLVHDDVG
                     SNMAAHVIQTKGDYDRARAQADVIIKRRFLYDRGTAAPMEGRGVVAQFDPLARHLTVW
                     DSTQAPIPIRNGLAGMLGLAERQVRVVAPFVGGGFGPKIMMFYPEETLVPWAAMRLGR
                     PVKWIEDRLENFTAMTQERGQVHESEMALTRDGRILGVKDVFLHDTGAYDPYGLTVPL
                     NTQCTLLGPYVIPDYYTEFKAVFTNKTIVTPYRGAGRQHGVFVVERLLDFAAKELGLD
                     PVEIRRRNLIPPDKFPWSNEIIFQDFTKLTYDSGNYEPILDKTLQMIGWDTFEQEEGA
                     RLRAEGKRVGIGVVMYVEGTGIGPYEGARIQIEATGKVTVVTGVGTQGQGHYTSFAQI
                     AAEQLGMPLKDVLVSTGDTGQFNWGTGTFASRGATVAGNAVHAAAVRVREKALQHASD
                     ALEAAVEDIELVDGYAQVRGVPTARISLADLARKANPLRGAVQPGTEPGLEATAYFGP
                     PMGATAAGAHALIL"
BASE COUNT          387 a          851 c          790 g          327 t
ORIGIN      
        1 ccgacgtcga gccccgcacg catctcggcg acctgctgcg cgacgagctg cagctgacgg
       61 cgacgcacct gggctgcgag cacggcgtgt gcggcgcctg caccgtgttg ctcgacggcg
      121 tgccggtgcg ctcgtgcctc accttcgcgg tgcaggccgg ggggcaggag gtgggcaccg
      181 tggagggcct ggccgccgac cccgagcacc tgcatccgtt gcagcaaggc ttctgggaag
      241 cccacggcct gcaatgcggc ttctgcacgc ccggcttcct gatgaccctg ctgccgttcc
      301 tggtcgagca ccccgacccc agcgagaccg agatccgcga ggcgctgtcg ggcaacctct
      361 gccgctgcac cggctaccag aacatcgtgg ccgccgtgca actggccgcg cgaaagctgc
      421 gcgcccccgc gggagacgcc tcatgagcac ccagatgttc ggcgcgcgca tcaagcgcag
      481 cgaagacccg caactgctga ccgggcgggc gcagttcgtg gacgacgtgc atatccccgg
      541 catggtccac gccgctttca agcgcagcga ctacgcccac gcccgcatca ccggcatcga
      601 cgtggaggcg gcgcgcgccc tgcctggcgt gctggccgtc tacaccgccg ccgacctggg
      661 ggactacttg cagccgtcgc ccctgctggt gcagcccccg cctacgcagc acatcgtctt
      721 caaccagcgc acggccaccc aactggccca ggacaaggtc cgctatgtcg gcgagcccat
      781 cgccatggtc gtggccgaga gccgctacct ggccgaagac gccgccgagc agatcgtggt
      841 cgattacgag ccgctgcctg ccgtagtgga cctggaagcg gccatggcgc ccgccgcgcc
      901 gctggtgcat gacgatgtcg gctcgaacat ggcggcgcat gtcatccaga ccaagggcga
      961 ctacgaccgc gcgcgcgccc aggccgacgt gatcatcaag cgccgcttcc tctacgaccg
     1021 gggcaccgcc gcgccgatgg agggccgggg cgtggtcgcg cagttcgacc cgctggcccg
     1081 ccacctcact gtctgggact cgacgcaggc gcccatcccg atccgcaacg ggctggccgg
     1141 gatgctgggc ctggccgagc ggcaggtgcg cgtggtcgcg cctttcgtgg gcggcggctt
     1201 cggccccaag atcatgatgt tctacccgga ggaaacactg gtgccctggg cggccatgcg
     1261 cctggggcgc ccggtcaagt ggatcgagga ccgcctggag aacttcaccg ccatgaccca
     1321 ggagcgcggg caggtccacg agtccgagat ggcgctgact agggacgggc gcatcctggg
     1381 cgtgaaggat gtgttcctgc atgacaccgg cgcctacgac ccctacggcc tgaccgtgcc
     1441 gctcaacacg caatgcaccc tgctggggcc gtatgtcatc ccggactact acaccgagtt
     1501 caaagccgtc ttcaccaaca agaccatcgt cacgccctac cgcggcgcgg ggcggcagca
     1561 tggcgtgttc gtagtcgagc gcctgcttga cttcgccgcc aaagagctgg gcctggaccc
     1621 ggtggagatc cgccgccgca acctcatccc gcccgacaag tttccctggt cgaacgagat
     1681 catcttccag gacttcacca agctgaccta cgacagcggc aactacgagc cgatcctgga
     1741 caagaccctc cagatgatcg gctgggacac gttcgagcag gaggaaggcg cccggctgcg
     1801 cgccgagggt aagcgcgtgg gcatcggcgt ggtgatgtac gtcgagggca ccggcatcgg
     1861 cccctacgag ggcgcgcgca tccagatcga ggcgaccggc aaggtcacag tggtgacggg
     1921 cgtgggcaca cagggccagg gccactacac cagcttcgcc cagatcgccg ccgagcaact
     1981 gggcatgccg ctgaaggacg tgctcgtgtc caccggcgac accgggcagt tcaactgggg
     2041 caccggcacc ttcgccagcc ggggagccac cgtcgcgggc aacgctgtcc acgccgccgc
     2101 cgtgcgtgtg cgcgagaagg cgctacaaca cgcctctgac gcactggagg cggcggtgga
     2161 ggacatcgaa ctggtggacg gctacgccca ggtgcgcggc gtgcccacgg cgcggatcag
     2221 cctggccgac ctggcccgca aggctaaccc gttgcgcggc gccgtccaac ccggcaccga
     2281 acccggcctc gaagccacgg cctacttcgg cccgcccatg ggcgcgaccg cggcgggcgc
     2341 ccacgcgctg atcct
//
LOCUS       DASUYC010001003         3993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_825354,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001003 DASUYC010000000
VERSION     DASUYC010001003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825354"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1203)
                     /locus_tag="VF276_16385"
     CDS             complement(<1..1203)
                     /locus_tag="VF276_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX6608481.1"
                     /translation="MDLFDYATQKQQQQDAPLAARMRPRTLDEIVGQTHLLAPGRAFR
                     RAIEADRVGSLILWGPPGSGKTTLARIIAATTRAHFAPVSAVSAGVADLRKIVKEAQE
                     RRRLGGGRTILFIDEIHRFSKSQQDAILPYVEDGTISLIGATTENPSFEVNSALLSRS
                     RVVQLFPLTDADLGVLLDRAVTDEERGLGGQHITLAPEARAHLLALSSGDARVLLNTL
                     EIASGGLEPGEDGQALLDLAAVEDAVQRRALQYDKGGEQHYDLISALHKSVRDSDPDG
                     SIYWLGRMIEAGEDPLFLARRLIRMSVEDIGLADPQALPLAMAAQQAVHFLGVPEGLL
                     ALAEIAVYLALAPKSNALYRAYGQVEQDVATTRNDPVPLHLRNAPTALMAGLGYGKGY
                     KYAHDYENA"
     gene            complement(1218..1799)
                     /locus_tag="VF276_16390"
     CDS             complement(1218..1799)
                     /locus_tag="VF276_16390"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEX6608482.1"
                     /translation="MAYCPNCGWQHDGTAHFCQQCGRPLGATAAAPPTAPMPAPPTGA
                     YGPGYAAPAPPVGDTPDWISEEHDLWLGKTIDMATQGALSPNHYRLTTRSLFYARGRI
                     GSVENSVPLWAVKGVTIDQSIVDKARQVGSLIVHVEHDDWTEGVTEVRLEDIENPQEI
                     RDLILRQAREENYNYERRNQTMFYQGRPPYPPR"
     gene            complement(1831..3516)
                     /locus_tag="VF276_16395"
     CDS             complement(1831..3516)
                     /locus_tag="VF276_16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX6608483.1"
                     /translation="MEPIWLAHYDQGVPARLTYPDGPLQDLLENAARTHPDNIATIFF
                     GARMTYREIEDESNRLAHALQKLGIGKGDRVALVLPNCPQFVVSYYAALKAGAVVVPT
                     NPTYKPREFQHQFADAGVRLVITLNLFVPAIQEIQPETAIKDIIVARIQDYLPTMLSY
                     LYPIKEKREGTAVHVTPGPGINDFCTLIRATSPDYAPVPVAQTDLAVLQYTGGTTGTS
                     KGAMLTHRNLVANALQVRSWLPDVREDGAEVFMAVTPFFHVYGMTVVMNFAILVGAAM
                     VLLPRFNTKEVLHNIQRYKATTFGGVPTMYIAINNFPEIKKYKLSSVKACISGAASLP
                     LEVAQQFEELTGGRLREGYGLTEASPVTHCNPIYGQPKAGSIGVPFPDVEAKIVDLED
                     AHDLPLGEIGELAIRGPQVMAGYWNRPEESANVLRDGWLLTGDTGRMDEDGFFYIVDR
                     KKDIIIAAGFNIYPREVEEVLYEHPAVKEAVVAGVPDAYRGETVKAYIVLKEGATATA
                     DEIIAFCRERLAGFKAPRIVEFRESLPKSLIGKHLRRVLIEEERAKQAAPVTA"
     gene            complement(3616..>3993)
                     /locus_tag="VF276_16400"
     CDS             complement(3616..>3993)
                     /locus_tag="VF276_16400"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEX6608484.1"
                     /translation="PGTGRIVAGVRDPQGGFVPTLDLMAAQAAAAMPAPNTVIGLVAT
                     NAPLDPTALSVVARMANSGLARAITPAHTMYDGDTLFALALPAAPLAGRADPGLVTLA
                     GSLAAEAVSRAVVRAVSSDGAVA"
BASE COUNT          595 a         1313 c         1392 g          693 t
ORIGIN      
        1 ggcgttctcg taatcgtggg cgtacttgta gcccttgccg tagcccaggc ccgccatcag
       61 cgcggtcggg gcgttgcgca ggtgcagcgg cacggggtca ttgcgcgtgg tcgccacgtc
      121 ctgctccacc tgcccatagg cgcggtagag agcgttgctc ttgggcgcca gggccaggta
      181 gacggcgatc tcggccaggg ccagcagccc ctccggcacg ccgaggaagt ggaccgcctg
      241 ctgcgcggcc atcgccaggg gcaatgcctg cgggtcggcc aggccgatat cctcgacgga
      301 catgcggatc aggcggcgcg ccaggaagag cgggtcctcc cccgcctcga tcatgcggcc
      361 cagccagtag atcgatccgt cggggtcgga gtcgcgcaca gatttatgta aagccgagat
      421 cagatcgtag tgctgctcgc cgcccttgtc gtattgcaag gcgcggcgct gcacggcgtc
      481 ttccacggcg gccagatcca gcagcgcctg accgtcctcc cccggctcca gcccgccgct
      541 ggcgatctcc agcgtgttga gcagcacgcg ggcgtcaccg ctgctgagcg ccagcaggtg
      601 ggcgcgcgcc tcgggcgcca gagtgatgtg ctgcccgccc aggccgcgct cctcgtcggt
      661 cacggcgcgg tcgagcaaca cacccaggtc ggcatcggtc agcgggaaca gttgcaccac
      721 gcgtgagcgc gacagcagcg cgctgttgac ctcgaacgac gggttctcgg tggtggcgcc
      781 gatcaggctg atcgtgccgt cttcgacgta gggcaggatg gcgtcctgct gcgacttgga
      841 gaagcggtgg atctcgtcga tgaagaggat ggtgcggccc ccgcccaggc ggcggcgctc
      901 ctgggcctcc ttgacgatct tgcgcaggtc ggccacgccc gccgagacgg cgctgacagg
      961 cgcgaagtgg gcgcgggtgg tggcggcgat gatgcgggcc agcgtggttt tgccgctgcc
     1021 cggcggcccc cagaggatca gcgacccgac gcgatcagct tcgatggccc gccggaaggc
     1081 gcgccccggc gccagcaggt gcgtctggcc cacgatctcg tccagcgtgc gcgggcgcat
     1141 gcgcgcggcc aggggcgcat cctgctgttg ctgcttttgc gtggcgtagt cgaacagatc
     1201 cacggcagcc accggaccta ccggggcgga tacggcggac gaccctggta gaacatcgtc
     1261 tgattccgcc gctcgtagtt gtagttctcc tcgcgcgcct ggcgcaagat caggtcccgg
     1321 atctcctgcg ggttctcgat gtcttcgagc cgcacctcgg tcacaccctc ggtccagtcg
     1381 tcgtgctcga cgtggacgat cagcgaaccg acctgccgcg ccttatccac gatgctctga
     1441 tcgatggtga cgcccttgac ggcccatagg ggcacgctgt tttcgacgct gccgatgcgg
     1501 ccccgcgcgt agaacaggct gcgcgtggtc agccggtagt ggttgggcga gagggcgccc
     1561 tgcgtcgcca tgtcgatggt cttgcccagc cagagatcat gctcctcgct gatccagtcg
     1621 ggtgtgtcgc ccacgggcgg ggccggagcg gcatagccgg ggccataggc cccggtgggt
     1681 ggcgcgggca tgggcgcggt gggcggggcc gccgccgtgg cgcccagcgg cctgccgcat
     1741 tgctggcaga agtgcgccgt gccgtcgtgc tgccacccac agtttggaca ataggccatg
     1801 cggttccctt tctctccagc acccgcgtga ctacgcggtg acgggcgcgg cctgtttggc
     1861 gcgctcttcc tcgatcagca cccgccgcaa gtgcttgccg atcaggctct tgggcaggct
     1921 ttcgcggaac tcgactatgc gcggcgcctt gaacccggcc aggcgctcgc ggcagaaggc
     1981 gataatctcg tcggcggtgg ccgtcgcgcc ctctttcagc acgatgtagg ccttgactgt
     2041 ctcgccgcga taggcgtcgg gcaccccggc caccaccgct tccttcaccg ccgggtgctc
     2101 gtagagcact tcctcgacct cgcgcgggta gatgttgaac ccggcggcga tgatgatgtc
     2161 cttcttgcgg tcgacgatgt agaagaagcc gtcctcgtcc atgcggcccg tatcgccggt
     2221 cagcaaccag ccgtcgcgca gcacgttcgc gctctcttcg ggccggttcc agtagccggc
     2281 catgacctgc gggccgcgga tcgccagttc gccgatctcg cccagcggca gatcgtgggc
     2341 gtcctccaga tcgacgatct tggcttccac atcggggaag ggcacgccga tggagccggc
     2401 cttgggctgg ccgtagatcg ggttgcagtg ggtgacgggc gatgcttcgg tgaggccgta
     2461 gccctcgcgc agccgcccgc cggtcaactc ctcgaactgc tgggccacct ccagcggcaa
     2521 cgatgccgcc ccgctgatgc aggctttgac gctcgacagc ttgtacttct tgatctctgg
     2581 gaagttgttg atggcgatat acattgtcgg cacgccgccg aaagtggtcg ccttgtaccg
     2641 ctggatgttg tgcagcactt ccttcgtatt gaagcgcggc agcaacacca tcgccgcccc
     2701 cacgaggatg gcgaagttca tcaccacggt cataccgtag acgtggaaga agggcgtcac
     2761 ggccatgaag acctccgcgc cgtcctcacg cacatccggc agccaggacc gcacctgcag
     2821 tgcgttggcg accaggttgc ggtgcgtgag catggcgccc ttggaggtgc ccgtcgtgcc
     2881 gccggtatat tggagcacgg ccaggtcggt ctgcgccacc ggcaccgggg cgtagtcggg
     2941 gctggtcgcg cggatcagcg tgcagaagtc gttgataccc ggccccggcg tcacgtggac
     3001 cgccgtgccc tcgcgctttt ccttgatcgg atagaggtac gagagcatcg tcggcaagta
     3061 gtcctggatg cgggccacga taatgtcttt gatggccgtt tccggctgga tctcctggat
     3121 ggcgggcacg aacagattga gggtaatcac caggcgcacg cccgcgtcgg cgaactggtg
     3181 ctggaactcg cgcggcttgt aggtcgggtt ggtgggcacg acgacggcgc ccgccttgag
     3241 cgcggcgtaa tagctgacga cgaactgcgg gcagttgggc agcaccagcg ccacccggtc
     3301 gcccttgccg atgcccagct tttgcagcgc gtgggccagc cggttcgact cgtcttcgat
     3361 ctcccggtaa gtcatgcgcg cgccgaagaa aatcgtggcg atgttatccg ggtgggtgcg
     3421 ggccgcgttt tccagcaaat cctgcagcgg cccatcgggg taggtgaggc gcgccgggac
     3481 gccctgatcg tagtgtgcca gccagatcgg ttccattgtg ttgctccttt gcaatacagt
     3541 agaagacaaa aacggactac taagagcgaa tggcgacccg agggcctccc tgtgcggcac
     3601 tacgcaacta tgcggtcagg cgaccgcgcc atccgaagaa accgcgcgca cgaccgcccg
     3661 gctcacggct tccgcggcca ggctgccggc taacgtcacc aaccctgggt cggcgcgccc
     3721 ggcgagcggc gcggcgggca gagccagggc gaagagggta tccccgtcgt acatggtatg
     3781 ggcgggggtg atggcgcgcg cgagcccgct gttcgccatg cgcgcgacca cgctgagcgc
     3841 ggtggggtcg agcggggcat tcgtcgcgac gaggccgatc acggtgttgg gcgcgggcat
     3901 ggccgcggcg gcctgggcgg ccataaggtc gagcgtcggc acgaagccgc cttgcgggtc
     3961 gcgcactccc gccacgatcc ggccggtgcc cgg
//
LOCUS       DASUYC010001004         2084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1584796, whole genome shotgun sequence.
ACCESSION   DASUYC010001004 DASUYC010000000
VERSION     DASUYC010001004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1584796"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2084)
                     /locus_tag="VF276_16405"
     CDS             complement(<1..>2084)
                     /locus_tag="VF276_16405"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6608485.1"
                     /translation="TPLPQGGVRVERMHAPPPVAAAPAPDLAPEVEPALRAYIPGAVL
                     ARLRAAETGWLAELRRVTVIFLNLPAFSHTLTLDQAQTVMHALQTILYRYEGTINKLS
                     VDDKGASLVAAFGLPPLAHEDDTARGAQAALAMAAYLREAGMPGAIGVTTGRAFCGIV
                     GSPERREYTMIGAVVNLAARLMQAAPGAILCDAATYLAAQAQVEFAALPPIYVKGRTE
                     AVPVYRPLGVVRAVEVPQPALIGRAAERATLDAALGRLTHGGDGGVVFIEGEAGIGKS
                     RLALALRDDAGGQDITVLGGAGNAMDQATAYHAWRPVFDAVFDRYGARTAEARRERVL
                     AALAALPDGPRLAPLLGTVLQLDLPETDITAQMSAQVRAENTRALLVGLLLMVARRGP
                     ALLILEDAQWMDSASWALAVEAANRVRSLLLVVVTRPPGDPPPAEYRALLATPGAQRI
                     RLDSLPPAETLALVSARLGVAGLPEPVAALIRDKAEGHPLFSEELAYALRDAGLLIVA
                     DGECRLAPGAGDLRALTFPDNVQGVITSRIDRLTPQQQVTLKAASVIGRVFPVRVLRD
                     IHPIEDDKPHLVRELQTLDRLNLTLRDSPPPDLAYMFKHVITQEVAYNLMLFAQRRQL
                     HRAVAGWYEARYGADLAPYYAILAYHWGKAEVTPKTLFYLEKAGQAALHSYANQEAIA
                     FFRDLLALHARA"
BASE COUNT          273 a          716 c          814 g          281 t
ORIGIN      
        1 ccgcccgcgc gtgcagggcc agcaggtcgc ggaagaaggc gatcgcttcc tggttggcgt
       61 agctgtgcag cgcggcctgg cccgccttct ccaggtagaa cagcgttttg ggcgtcactt
      121 cggccttgcc ccagtggtag gccaggatgg cgtaataggg cgcgaggtcg gctccgtagc
      181 gcgcctcgta ccacccggcc accgcgcggt gcaactgccg ccgctgggcg aacagcatca
      241 ggttataggc gacttcctgg gtgatgacgt gcttgaacat atatgccagg tcgggcggcg
      301 gcgagtcgcg cagggtcagg ttgagccggt cgagcgtctg gagttcgcgc accaggtggg
      361 gcttgtcgtc ttcgatgggg tggatgtcgc gcagcacgcg caccgggaac acccgcccga
      421 tcacactggc cgccttgagc gtgacctgct gctgcggcgt cagccggtcg atgcggctgg
      481 tgatcacgcc ctgcacgttg tcggggaagg tgagggcgcg cagatcgccc gcgccgggcg
      541 cgaggcggca ctcgccgtcc gccacgatca gcagcccggc gtcgcgcagg gcgtaggcca
      601 gttcctcgct gaacagcggg tggccctcgg ccttgtcgcg gatcagcgcg gccaccggct
      661 ccggcaggcc ggccacgccc aggcgggcgc tgaccagggc cagcgtctcg gcgggcggca
      721 ggctgtcgag gcggatgcgc tgggcgccgg gcgtggcgag cagcgcgcgg tactcggcgg
      781 gcggcgggtc gccgggcggg cgggtgacga cgacgagcag cagcgagcgc acccggttgg
      841 ccgcctccac ggccagcgcc caggatgccg agtccatcca ttgcgcgtct tcgaggatga
      901 gcagggcggg gccgcgccgg gcgaccatca gcagcaggcc caccagcagg gcgcgggtgt
      961 tctcggcgcg cacctgggcg ctcatctggg cggtgatgtc ggtttccggc agatcgagtt
     1021 gcagcacggt gccgagcaag ggcgccaggc gcgggccgtc gggcagggcg gcgagggcgg
     1081 ccagcacgcg ctcgcgccgg gcctcggcgg tgcgcgcgcc gtagcggtcg aacacggcgt
     1141 cgaagaccgg gcgccaggca tgatacgcgg tggcctggtc catggcgttg cccgccccgc
     1201 ccagcacggt gatgtcctgg ccgccggcgt cgtcgcgcag ggccagcgcc agccgcgact
     1261 tgccgatgcc cgcctcgccc tcgatgaaca ccacgccgcc gtcgccgccg tgtgtcaggc
     1321 gccctagcgc ggcgtccagc gtggcccgct cggcggcgcg gccaatgagc gcgggctgcg
     1381 gcacctccac ggcgcgcacc acgcccagcg ggcgatacac cggcacggcc tcggtgcgcc
     1441 ccttgacata aatcggcggc aaggcggcga actcgacctg cgcctgggcg gccaggtagg
     1501 tggcggcatc gcacaggatc gcgccgggcg ccgcctgcat cagccgggcg gccaggttga
     1561 ccaccgcgcc gatcatcgtg tactcgcgcc gctcggggct gccgacgatg ccgcagaagg
     1621 cgcgccccgt ggttacgcca atggcgccgg gcatcccggc ctcgcgcagg taggcggcca
     1681 tcgccagggc ggcctgggcg ccgcgcgccg tgtcgtcctc gtgggccagc ggaggcaggc
     1741 cgaacgccgc caccagcgag gcgcccttgt cgtccacgct gagcttgttg atggtgccct
     1801 cgtagcggta gaggatggtt tgcagcgcgt gcatgacggt ttgcgcctgg tcgagcgtca
     1861 gggtgtggct gaaggcgggc aggttgagga agatgacggt gacgcgccgc agttcggcca
     1921 gccagccggt ttcggcggcg cgcaggcggg ccagcaccgc gccggggata taagcgcgca
     1981 gggccggctc gacttcgggc gcgaggtccg gcgcgggcgc ggcggcgacg ggcggcggcg
     2041 cgtgcatccg ctccacacgc accccgccct gcggtaacgg cgtc
//
LOCUS       DASUYC010001005         2425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1786397, whole genome shotgun sequence.
ACCESSION   DASUYC010001005 DASUYC010000000
VERSION     DASUYC010001005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1786397"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VF276_16410"
     CDS             <1..400
                     /locus_tag="VF276_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX6608486.1"
                     /translation="GKMAEIAPRTALFEHPQHPYTQALLSAIPMPDPRNRRQRIVLKG
                     DVPSPLNPPTGCRFHTRCWLAQDICKQQIPEWRNVGGEGPAAHWVACHFAPFTRIPTP
                     SEVGSTAAVVKVVSHDTPAPTDTPKVETPA"
     gene            448..828
                     /locus_tag="VF276_16415"
     CDS             448..828
                     /locus_tag="VF276_16415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6608487.1"
                     /translation="MDMKLELVPMPVSDVDRAKAFYVEQVGFHADHDVRPNDTVRVVQ
                     LTPPGSACSIVLSTGLPGIEMAPGSLRGLHLVVADIRQARAALMGRGVEVSEIDEQGR
                     GIKYASFSDPDGNTWALQEIPPGI"
     gene            1155..2111
                     /locus_tag="VF276_16420"
     CDS             1155..2111
                     /locus_tag="VF276_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015154370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6608488.1"
                     /translation="MQGTRTVRERDAETRGLAYGFLGVLGFSLTLPATRVAVAYFDPT
                     AVGLGRALVAAGLAALLLLATRQTWPSRPQWRSLVIVAAGVILGFPLLSAWAMSRLPA
                     AHGAVILGLLPLATALAGALRAGERPSRGFWVAGVAGSATVVAFAVSMGAGDIQPADL
                     ALFGAVLAAALGYAEGGRLARELGGWQVISWALVLAAPFIAVPVFLALGNPAPPAPWS
                     AWFSFGYVAVVSQFLGFFAWYRGLALGGVARVSQLQLLQPFLTLLASALLLGEQITLL
                     MVGAALVVLVTVALGRKAAIVRPAAPPPATPATLAETPPPVR"
     gene            complement(2125..>2425)
                     /locus_tag="VF276_16425"
     CDS             complement(2125..>2425)
                     /locus_tag="VF276_16425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608489.1"
                     /translation="DLNNYFSSPNKLFDFIQAGVPIVASDLPFLRKVITETDGGLVAR
                     LDSPEAYAATINAVLTRPDGGAAVRANLRRAAPAYTWETQSRKLIAAYAALARGD"
BASE COUNT          344 a          869 c          804 g          408 t
ORIGIN      
        1 gggcaagatg gccgaaatcg caccgcgcac ggcgctgttc gagcacccgc agcaccccta
       61 cacgcaggcc ctcctgtcgg ccatccctat gcccgatccg cgcaatcgcc gccagcgcat
      121 tgtgttgaag ggcgacgtgc ccagcccgct caacccgccc accggctgcc ggttccatac
      181 ccgttgctgg ctggcgcagg acatctgcaa gcagcagatt cccgagtggc gcaacgtggg
      241 cggcgagggc cccgccgcgc actgggttgc ctgccatttc gcgccgttca cgcgcatccc
      301 gacgccgagc gaagtcggca gcaccgccgc cgtggtcaaa gtcgtcagcc acgacacccc
      361 ggcccccacc gacacgccga aagtcgaaac gcccgcctga cgcaccgttc cgcccggtta
      421 gctgcgtgca ggagagcaag actaataatg gacatgaaac ttgagttggt cccgatgccg
      481 gtgtccgatg tggaccgcgc caaggctttc tacgtggaac aggtcggatt tcacgccgac
      541 catgacgtgc ggcctaatga tactgtccgc gtggtccaac tcacgccccc cggatcggcg
      601 tgttccatcg tcctcagcac cggcctgccc ggtatcgaaa tggcgccggg ctcgctgcgg
      661 ggcctgcatc tggttgtcgc agacattcgc caggcacgcg ccgcgctcat gggtcgcggc
      721 gtggaagtca gcgagatcga cgagcagggc aggggcatca agtacgcgtc cttcagcgac
      781 cccgacggca acacctgggc cttgcaggaa attccacccg gcatttaacc aggggtttac
      841 caccgacctc cctcatcgcg accaaccgga ttgggtacac gctacccggt ccggtttgtt
      901 gtctgccgca ttccgcgata gcccctacct gcgccttgcc gggaatcgcc gccccgtagc
      961 ggccaacttg aacccagccg ctacacccgc gccgcccagc caccacagct cattgtagca
     1021 ccggtttgaa accggcccgg cgcaccacga cctcactggc cgattgcgcg tccgcaagga
     1081 tagacagggc cgcagtgcat ctgctataat ggaaaacgtc tacgatagcg tactgagtgg
     1141 aagcgaggaa cgccatgcag ggtacccgga cagtgcgtga gcgggacgcg gaaacgcggg
     1201 ggttggccta tggtttcctg ggcgtgctcg gcttcagcct gactctgccc gccacccgcg
     1261 tcgccgtggc ctactttgac ccgacggcgg tcgggctggg gcgcgccctc gtggcggcgg
     1321 ggctggccgc gctgctgctg ctcgctaccc gccagacctg gccatcgcgc ccgcagtggc
     1381 gcagcctcgt catcgtcgcc gccggggtca tcctgggttt cccgttgctc tcggcctggg
     1441 ccatgagccg cctgcccgcc gcgcacggcg ccgtcatcct cggcctgttg cccctggcga
     1501 cggcgctggc cggggcgttg cgggccggag agcgcccttc gcgcggcttc tgggtggccg
     1561 gggtggccgg cagcgccacg gtggtcgcct tcgccgtcag catgggcgcc ggcgacatcc
     1621 agcccgccga cctggcgctg ttcggggcgg tgctggccgc ggccctgggc tacgctgagg
     1681 gcgggcggct ggcccgcgaa ctcggcgggt ggcaggtgat tagctgggcg ctggtgctgg
     1741 ccgcgccgtt catcgccgtg cccgtgttcc tggccctggg caaccccgcg ccgcccgcgc
     1801 cctggtcggc ctggttcagc ttcggctatg tcgcggtggt cagccagttc ctcggtttct
     1861 tcgcctggta tcgcggactg gcgctcggcg gcgtcgcgcg ggtcagccag ctccagctct
     1921 tgcagccgtt cctcaccctg ctggcctcgg cgctcctcct cggcgagcag atcaccctgc
     1981 tcatggtggg tgcggcgctg gtggtgctgg tgacggtcgc gctgggtcgc aaggccgcca
     2041 tcgtccgtcc cgcagccccg ccgccggcga cgcccgctac gcttgccgaa accccgccgc
     2101 cggtgcggtg aatccctggt gcggttaatc cccgcgggcc agggcggcgt aggccgcgat
     2161 cagcttgcgg ctctgcgtct cccaggtata cgccggggcg gcgcgccgga gattggcgcg
     2221 cacggcggcc ccgccgtccg ggcgggtcag cacagcgttg atcgtcgccg cataggcttc
     2281 ggggctgtcg agccgcgcca cgagaccgcc atccgtctcc gtgatcacct tgcgcaagaa
     2341 gggcagatcg ctggcgacaa tcggcacgcc cgcctggata aagtcgaaca gcttgttggg
     2401 cgagctaaag tagttgttca gatcc
//
LOCUS       DASUYC010001006         2032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1786882, whole genome shotgun sequence.
ACCESSION   DASUYC010001006 DASUYC010000000
VERSION     DASUYC010001006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1786882"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1139)
                     /locus_tag="VF276_16430"
     CDS             complement(<1..1139)
                     /locus_tag="VF276_16430"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX6608490.1"
                     /translation="MNILHLLHDPLEELSKVLARLGQQWTAVLGLPVALAVVLVYGLP
                     RLARRAGAAQDARLLREAGLLALGWGGVDLALTAGLPLLRLSYAPVRTAFFMLLWGRT
                     GISGAGTALAVASVLAGRDLPPGGRRLIARGLWAMHAAMALVLVYATYVGGARLRPTR
                     ARLAFPGHTPGAPPLHLRLAQISDIHMERLSRRDEAMVALLREARPDLILLTGDFINI
                     DYYDVRAYEDLRALLSAVSALNPPLGVYACLGNVDPPKLTGTLLESAGVHLLVDTAVT
                     LPVDGRRVQILGAATSRGHRWEYDLPHFQAAVAAAEAQGTPDFRLLLYHTPDFVPQAA
                     RAGVDLYLCGHTHGGQIRLPLVGALRTGSRFGKRYVIGLNRLPNGG"
     gene            1500..1817
                     /locus_tag="VF276_16435"
     CDS             1500..1817
                     /locus_tag="VF276_16435"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX6608491.1"
                     /translation="MLVAIVRIPAPADPEAAAAMERRFAERLPGVQQSPGFAGFELLR
                     PSEGTREYLSISRWQTRADFDAWANSAANAQAHGRPASAAPGGHGGASLPPGVELYEV
                     SET"
     gene            1838..>2032
                     /locus_tag="VF276_16440"
     CDS             1838..>2032
                     /locus_tag="VF276_16440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608492.1"
                     /translation="MATGKTTEPQLIPRAVLFGNPVKTAPRLSPDGTRMAYLAPVDNV
                     LNVWAGPVDGSNFQPVTKDTD"
BASE COUNT          349 a          764 c          660 g          259 t
ORIGIN      
        1 ccgccgttgg gcagccggtt gaggccgatg acatagcgct tgccgaagcg tgaccccgtg
       61 cgcagcgcgc cgaccagcgg caggcggatc tggccgccgt gcgtgtgccc gcacaggtag
      121 agatccacgc cggcgcgagc agcctgcggg acgaaatcgg gcgtgtggta gagcagcagg
      181 cggaagtcgg gcgtgccctg tgcttccgcc gccgccaccg ccgcctggaa gtgcggcagg
      241 tcgtactccc agcggtgccc gcggctggtc gccgccccca ggatctgcac ccgtcgcccg
      301 tccaccggaa gcgtcacggc ggtatcgacc agcagatgga cgcccgcgct ttccagcaag
      361 gtgcccgtca gtttgggcgg gtcgacgttg cccaggcagg catagacgcc cagcggcggg
      421 ttcagcgccg agacggcgct cagcagcgcg cgcaggtcct cgtaggcccg cacatcgtaa
      481 tagtcgatgt tgatgaaatc cccggtgagc aggatcaggt cgggccgggc ctcgcgcaac
      541 agcgccacca tcgcctcgtc acggcggctc aggcgctcca tgtggatgtc cgagatctgg
      601 gccagccgca ggtgcagcgg cggggcgccg ggcgtatggc cggggaaggc cagcctggcg
      661 cgggtcgggc gcaggcgcgc cccgcccacg taggtcgcgt agaccaggac cagcgccatg
      721 gcggcgtgca tggcccagag tccacgggcg atcaggcggc gcccgcccgg cggcagatcg
      781 cgcccggcca gcacgctcgc cacggccagc gccgtgcccg cgcccgagat ccccgtgcgg
      841 ccccagagca acatgaaaaa ggcggtgcgc accggcgcgt acgacaggcg cagcagcggc
      901 aaccccgccg tcagcgcgag atccacgccg ccccagccca gcgccagcaa cccggcctca
      961 cgcagcaggc gcgcgtcctg cgccgccccc gcccgccggg ccagccgcgg caacccgtag
     1021 accagcacca cggccagcgc caccggcagg cccagcaccg ccgtccactg ctgccccagg
     1081 cgcgccagga ctttgctcaa ctcctccagc ggatcgtgta atagatggag aatgttcaag
     1141 tgacctcctg aatcccgttc cttgtctatc aatcgtcacg cgcggcgccc gcaatggtaa
     1201 cgcgcagatc gcccccgata tgacagccac ccggcggggt cgcccggccc gtgaacgggc
     1261 gagcgaccac aacaaagcct gcctccgcag gctaggctga aggaaagaat aacaggccgc
     1321 gcaggcggcc ttcgtaaacc tagcctgccc gttcacgggc cgggcgaccc cgctccaccg
     1381 agatgcgcaa taggcaccca cgcccgcctg ccgccacccc ggcgaccagc cccgcgtata
     1441 tctgctataa tggagtattg tccacgcccc tatcacgatc ccccgaagga gggtttgcta
     1501 tgctagtcgc gattgtgcgt atccccgcgc cggccgatcc cgaagcggcg gcggcgatgg
     1561 aacgccggtt cgccgaacgg ctgcccggcg tgcagcagag ccccggcttt gccggctttg
     1621 aactgctgcg ccccagcgag ggcacccgcg aatatctctc gatctcccgc tggcagaccc
     1681 gcgccgattt cgacgcctgg gccaacagcg ccgccaacgc ccaggcccat ggccgccccg
     1741 catccgccgc acccggcggg cacggcggcg caagcttgcc gcccggagtc gagctatacg
     1801 aagtcagcga aacttagtgc ccagaggaga cagccccatg gctaccggca agaccaccga
     1861 gccgcaatta atcccccgcg ccgtgttgtt cggcaacccg gtcaagactg cgccccgcct
     1921 ctcgcctgac ggcacgcgga tggcctatct cgcgcccgtc gacaacgtgt tgaacgtctg
     1981 ggccggtccg gtggacggca gcaacttcca gcccgtcacc aaagataccg ac
//
LOCUS       DASUYC010001007         2114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1949506, whole genome shotgun sequence.
ACCESSION   DASUYC010001007 DASUYC010000000
VERSION     DASUYC010001007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1949506"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="VF276_16445"
     CDS             complement(<1..792)
                     /locus_tag="VF276_16445"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX6608493.1"
                     /translation="MGGIAAALAAASAAMIERVRPSVVQVQSEGRGGGAGVIWRSDGA
                     IVTNNHVVEHTSGTIQVVLTDGRSLEAQVVKTARSLDLALLQVDAQDLPAAPVADSSN
                     LRVGELVFAVGHPWGQKHVVTAGIVAAHGTVDPPGSGRSAHYVRTDARLAPGNSGGPL
                     VDAHGAVVGINAMIFGGDQGVAIPSHVVESWRAAEPSRRVYLGVEVRPIELPPAPRDE
                     RRRPRAAGLLVGTVHADGPANRAGLLAGDVLLEVDGRRLDGTDTLI"
     gene            complement(934..1845)
                     /locus_tag="VF276_16450"
     CDS             complement(934..1845)
                     /locus_tag="VF276_16450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX6608494.1"
                     /translation="MEQTTTGADTLGALSNAMADAVERAGAAVVQVNGRQRQSASGVV
                     FGPNLVLTADHVLEREDNLTVGTPDKRTLPAQFVGRDTSSDLAVLQVADLNINPITAG
                     QNPARVGQFILLVGRPSTDGLMASLGIISAVGGPVRTRRDRMLEQFIQTDAIPYPGFS
                     GGPMIDSSGAVLGLLTTGLANGAALAIPTAIAWRNAQTLSQHGTIKRGYLGISSQPVQ
                     LPEGQRAGWPGEIGLMIVRVEPDSPAAKGGLLLGDILVGLDGQVLSDTDDLQALLSGD
                     RVGKPVPIDVIRGGARQTVQVTIGERR"
BASE COUNT          288 a          734 c          759 g          333 t
ORIGIN      
        1 gatcagcgta tccgtgccgt ccaggcgccg gccgtcgact tccagcagca catcgcccgc
       61 cagcagcccc gcgcggttgg ccggtccatc cgcatggacc gtgcccacga gcaggcccgc
      121 ggcgcggggc cggcgccgct cgtcacgcgg cgcgggcggt agctcgatgg gccgcacctc
      181 cacgccgaga tagacccggc ggcttggttc ggcggcccgc cagctttcga ccacgtggct
      241 ggggatggcg acgccctggt cgccgccgaa gatcatggcg ttgatgccga cgacggcgcc
      301 gtgggcgtcc accagcgggc cgcccgagtt gccgggggcg aggcgcgcat cggtgcgcac
      361 atagtgggcg ctgcgcccgc tgccgggcgg atcgaccgtg ccgtgggcgg cgacgatgcc
      421 cgccgtcacg acgtgcttct ggccccaggg atggccgacg gcaaagacga gttcgcccac
      481 gcgcaggttg ctggagtcgg ccaccggcgc ggcgggcagg tcctgcgcgt ccacctggag
      541 cagggccagg tccaggctgc gggccgtctt gactacctgc gcctccaggc tgcgcccgtc
      601 ggtcagcacg acctggatcg tgccgctggt gtgctcgacg acgtggttgt tggtgacgat
      661 ggcgccgtcg gatcgccaga tcaccccggc gccgccgccc cggccctcgc tctgcacctg
      721 caccacgctg ggccgcacac gctcgatcat cgccgcgctg gccgcagcga gggcagcagc
      781 gatcccgccc acccttgcgc tcgccgccgt aaacacctcg gaaacttctc cggcagggac
      841 ttccgttaca gtatctgcta ctgtcatgct attcacctct gtgcgcgctc cggcgggcca
      901 tgcgctaagg caacccggtc cggtgcgctc gtattagcgc cgttcgccaa tggtgacctg
      961 gacggtctgg cgggcgccgc cgcggatcac gtcgatgggc accggcttgc cgacccggtc
     1021 gccgctgagc agcgcctgga ggtcgtcggt atcgctcagc acctggccgt cgaggccgac
     1081 gaggatgtcg ccgagcagca ggccgccctt cgcggcgggg ctgtcgggtt ccacgcggac
     1141 gatcatcagg ccgatctcgc ccggccaccc ggcgcgctgg ccctccggca gttgcaccgg
     1201 ctggctgctg atgcccaggt agccgcgctt gatggtgccg tgctgggaca gcgtctgggc
     1261 gttgcgccag gcgatagccg tggggatcgc cagggccgcg ccgttcgcca ggccggtggt
     1321 cagcaggccg agtacggcgc cgctgctgtc gatcatcggc ccaccggaga agccggggta
     1381 ggggatggcg tcggtctgga tgaactgctc cagcatgcgg tcgcggcgtg tgcgcaccgg
     1441 gccgcccacg gcgctgatga tgcccaggct cgccatcaac ccgtcggtgg acgggcgccc
     1501 gaccagcagg atgaactggc cgacgcgcgc cgggttctgc ccggcggtga tggggttgat
     1561 gttcaggtcg gccacctgca acacggccag gtcgctcgac gtgtcgcggc ccacgaactg
     1621 ggccggcagc gtgcgcttgt cgggggtgcc gacggtcagg ttgtcctcgc gttccagcac
     1681 atggtccgcg gtcagcacga gattggggcc gaagacgacg ccgctggccg actggcgctg
     1741 ccgcccgttg acctgcacga ccgccgcgcc cgcgcgctcg acggcatcgg ccattgcgtt
     1801 cgataacgcg ccaagggtat cggcgcccgt ggttgtttgc tccatggtct gtatcctccc
     1861 agataatgcg gagttccccc attgggaact tacccccact atacccgccg gggtgccgga
     1921 tgagcatcac ccggacggct agagggctac ccggacaaac ggctaggatc gggatggcgc
     1981 ggcgcgggcc ggtttgaaac tggcccctac atcggcgatc cggcgggcgc gggccggttt
     2041 gaaaccggcc cctacagata caatgatgga aatccgtgat tggtagccgg tgggcgagat
     2101 gttgcgggtt gcgg
//
LOCUS       DASUYC010001008         2933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_902539,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001008 DASUYC010000000
VERSION     DASUYC010001008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902539"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..946
                     /locus_tag="VF276_16455"
     CDS             59..946
                     /locus_tag="VF276_16455"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6608495.1"
                     /translation="MDSDSYRFQVGTFQCSCISDGTFDYPPEDFVAAVPTERFQEELQ
                     ARHLPTDHITSPYTSLLIDTGSHKVLIDTGAGFAPTNGHLLENLRAAGVAPTDIDTVI
                     LTHGHADHIGGNLDAEARPAFPNARYVMAQAEWEFWTGAPNLGPMLVAEPLKHLLIAT
                     AQRNLPPLRRQIDLVDGEIEIVPGIQAIPAPGHTPGHIALLITSGDQQLLHLADTVLH
                     PILVEHPDWYPRVDLLAEQAVATKRRLLDRAAADKLLTFVYHFAPFPSLGYVVAAGAA
                     WQWQPAVPAGELVGVVGGP"
     gene            993..1889
                     /locus_tag="VF276_16460"
     CDS             993..1889
                     /locus_tag="VF276_16460"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6608496.1"
                     /translation="MTEQLVLRDSQVRLPDGRTLAYAEYGEADGYPVLFFHGTPGSRV
                     QHPRMMMAGAAHSRIIAVERPGYGRTDFHTGWGMLDWADDVAALADALELARFAVVGF
                     SAGGPYAAACAYRWPARVTALGLLGSVAPWPGDSAGADRLPADDAGLDAWAVAARTLS
                     WPEYRVWRTEVERSTPPDGEQIWLERVAEMSGPVFGLPEVREALQRSVRETFRQGMEG
                     ILYDGWLLRRPWGFRLEDIAVPAYVWQCEQDPTGRLSGAQYLARTIPGCRAVYFPEEE
                     HWIPPHYWKGILSTLVGDRNAG"
     gene            1958..2452
                     /locus_tag="VF276_16465"
     CDS             1958..2452
                     /locus_tag="VF276_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX6608497.1"
                     /translation="MSTKQGDVGLLQDPVAQQLLQSTAPAHLAYTWTDGTPRVVPINF
                     HWNGAEIVLGGPPDAPKMKALQEGARVAVSIDSDHMPYKVLLIRGTIHIDTVEGVSPE
                     YAASCRRTLGEEAGNAWLAQVGGLMPRMTRIFIHPEWVAVQDFETRFPNAVEWAMERA
                     QVPA"
     gene            2625..>2933
                     /locus_tag="VF276_16470"
     CDS             2625..>2933
                     /locus_tag="VF276_16470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608498.1"
                     /translation="MAKVYNISLIALKPGVQGDEFERFVKEEIHSFQWPEGIQLLLLK
                     GDRGDREGKYLMMLEFESVARRNWASPANGKLSAEYLQMIEPVMAALNKWSTFASGPV
                     D"
BASE COUNT          514 a          938 c          956 g          525 t
ORIGIN      
        1 ccgcgccgta cttactccgc gcacccggag cctaccatcc gactgaagga agctacctat
       61 ggactccgac agctatcggt ttcaggtcgg gaccttccag tgtagttgca tcagcgacgg
      121 cacctttgac tatccgccgg aggacttcgt ggccgctgtg cccacggagc gatttcagga
      181 ggagttgcag gcccgccatc tacccaccga tcatattacc agcccctaca ccagcctgct
      241 aatcgacacc ggcagccaca aggtgctgat cgacaccggc gccggcttcg cccccacgaa
      301 cggccacctg cttgagaacc tgcgcgccgc cggggtcgct ccgacggaca tcgataccgt
      361 gatcctgacc catggccacg ccgatcatat tggcggcaat ctcgatgccg aagcgcggcc
      421 cgcgtttccc aacgcccgct acgtgatggc acaagccgag tgggagttct ggaccggggc
      481 gcccaatctg ggtcccatgc tggtcgcgga gccgctcaag cacctgttga tcgccaccgc
      541 ccagcgcaac ctccccccgc tgcgccgaca gatcgacctc gtggacgggg aaatcgagat
      601 cgtgccgggc atccaggcca ttcccgctcc cggtcatact cccggacaca tcgcgctcct
      661 catcacctcc ggcgaccagc agttgctcca tctggccgac acagtcctcc atccgatcct
      721 tgtggagcac cccgattggt atccgcgcgt cgatctcctg gccgagcaag cggtggccac
      781 caagcgccgg ctcctggacc gtgccgccgc cgataagctc ctgaccttcg tgtaccactt
      841 cgcgccgttt cccagtctcg gttatgtagt ggcggcgggg gccgcctggc agtggcagcc
      901 ggcggtgccg gcgggcgagc ttgtgggggt cgtaggcggc ccctgacccg cccttgctgt
      961 acggcaacga ttctacggag agaggtctag ccatgactga gcaactggtt ctgcgcgata
     1021 gtcaggtccg cttgcccgat ggccggacgc tggcctacgc cgagtatggg gaggctgacg
     1081 ggtatcccgt gctcttcttt catgggactc ccggctcgcg ggtgcaacac ccgcgcatga
     1141 tgatggccgg ggcggcccac agccggatca tcgcggtcga gcggccgggc tatgggcgta
     1201 ctgattttca taccgggtgg gggatgctgg actgggcgga cgatgtggct gccctagccg
     1261 atgcgctgga gcttgcgcgg ttcgcggtag tggggttttc ggcgggcggg ccctacgctg
     1321 ccgcttgcgc ctaccgctgg ccggcgcggg tcaccgcgct cggcctgctc ggcagcgtgg
     1381 ccccgtggcc cggcgacagc gccggcgccg atcggctccc ggcggacgac gccgggctgg
     1441 atgcctgggc tgttgccgcc cgcacactct cctggccgga gtaccgggtc tggcgaacgg
     1501 aggtcgaacg cagcaccccg ccggatggcg agcagatctg gctcgagcgg gtggctgaga
     1561 tgtcggggcc ggtgttcggg ctgcccgagg tgcgcgaggc cttgcagcgg tccgtgcgag
     1621 agactttccg acaaggcatg gagggcatac tctacgacgg ctggcttctg cgccggccct
     1681 ggggctttcg gctagaagat atcgccgtgc cggcctatgt ctggcagtgc gagcaggatc
     1741 caacgggtag actcagcggc gcccagtatc tcgctcggac catccccggc tgccgggccg
     1801 tgtactttcc cgaggaggag cactggatcc ccccccacta ttggaaaggg attcttagca
     1861 ccttagtcgg ggatcgcaac gccggttagg ggcacaataa tttccggcaa aaacggcaca
     1921 cgaacgacct atgttctaca ttagaaagga gcaccacgtg agcaccaagc aaggcgatgt
     1981 tggtctcctc caggaccccg tcgcgcaaca gctactccag tcgaccgcgc cggcccactt
     2041 ggcgtacacc tggacggacg gcactccccg cgtcgtgccg attaacttcc actggaacgg
     2101 cgcggagatc gtcctcggcg gcccgcccga cgcgcccaaa atgaaggcgc tgcaggaggg
     2161 ggccagggta gcggtcagca tcgattccga tcatatgccc tataaggtgc tgctcattcg
     2221 cggcaccatc cacatcgata ccgtcgaggg cgtgtccccc gagtacgccg ccagttgccg
     2281 gcgcaccctg ggcgaggagg cggggaacgc ctggctggcc caggttggcg gcctgatgcc
     2341 ccgcatgacg cgcatcttca tccaccccga gtgggtggcg gtgcaggact tcgagacgcg
     2401 cttccccaat gccgtcgaat gggccatgga gcgcgcgcag gtacccgcct agtatcagtg
     2461 tgttttcaaa tatggttgaa tcgctatatg aggagacacg aactgagcga cgaacaatgg
     2521 gcgaggcggc agttgcggct gccggcttcg cgcccccagg tggggcgacc cctcgtggat
     2581 caccgctagc ggatgctggc ggatcacgaa aggagggtct agcgatggcg aaggtgtata
     2641 acatcagctt gatcgcgctc aaacccggcg tgcaagggga tgagttcgag cggttcgtca
     2701 aggaagagat tcattcgttc cagtggcccg aaggtatcca gctcttgttg ttaaagggtg
     2761 atagaggaga tcgcgagggg aaatatctga tgatgctgga atttgagagc gtagcgcgcc
     2821 gcaattgggc atctccggca aacgggaagt tgtcggcaga gtacctacag atgatagaac
     2881 cggtgatggc agcgctgaac aagtggtcga cctttgcctc cggcccggtg gat
//
LOCUS       DASUYC010001009         6041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1342583, whole genome shotgun sequence.
ACCESSION   DASUYC010001009 DASUYC010000000
VERSION     DASUYC010001009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6041
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1342583"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VF276_16475"
     CDS             <1..442
                     /locus_tag="VF276_16475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6608499.1"
                     /translation="GAGLMLIGAAHDHRLAAVVGDSTYPVVGPMFGQWNSVGLRIWPY
                     RLSFAPLAEPTANLLLDSRLAELDPLEQAPNVSPSALLLIHAEHDHNGLTPLSGAREI
                     FAAAREPKALWISPIGDHAVIATANPDAYRAQVVGFFDHYLRGR"
     gene            complement(428..1537)
                     /locus_tag="VF276_16480"
     CDS             complement(428..1537)
                     /locus_tag="VF276_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608500.1"
                     /translation="MPDRIPPKARLPILAVGMVALLAAMWAGLLRLGWALPPLRDTLA
                     AAHGPLMVGGFLGTLIGVERAVALGRPWAYLAPLFTAAGAVVLLIGVPVPLGAGLLVA
                     GSVALLANYTAILRRQVALFTVTMALGADAWAIGNALWAVGRPIHQIVLWWAGFLVLT
                     IAGERLELSRLRRTSRASQALFLAAAGLFLAGLALSIVVYDAGARLAGAGMVALAAWL
                     ARYDVARRTVRLPGLPRFSAVCLLSGYVWLTAAGLMAVVFGGVAAGPRYDALLHAVFL
                     GYVFAMIFAHAPIILPAVTGLAVPFHRVFYAHLILLHLSLLLRVGSDLTGWLPGRQWG
                     GLLNVLVLLFFIGSTAVAVRRGRTKRPRPVSLPAA"
     gene            complement(1540..2964)
                     /gene="nirK"
                     /locus_tag="VF276_16485"
     CDS             complement(1540..2964)
                     /gene="nirK"
                     /locus_tag="VF276_16485"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HEX6608501.1"
                     /translation="MIQASKSVLIFAALAVPVLVALLVATATGTPAMPVPTPAAPGDH
                     DMAAMPASTAGPAAFTLRTGLDGGRMVYIGVGGAIDKVVNPTLEVPAGAAVAITLING
                     DAGEHDLVVPDFQAASARLRATGAQTTLQFTADKPGTFPYFCTLPGHRASGMEGRLAV
                     GPAATPQVAHAPSIVRDPTDLPPPLARRGPQAVEIHLEAVELEGQLASGTTYTYWTFN
                     GQVPGPFLRVRVGDTVTVYLKNRADSHMVHSIDLHAVAGPDGGAGLMQVAPGQEKAFT
                     FKALYPGLFVYHCATPMVASHLASGMFGMILVEPEGGLPPVDREFYVMQSELYTDQPF
                     GAPGRQNLSTDRLLAEQPTYFVFNGAVGALSDQHPLQAQVGQTVRIFIGDAGPNLPSS
                     FHVIGAVFDRVYNLGALESPPLTNVQAVLVPPGGSAMVEFGLHVPGRYMFMDHALSRA
                     ERGLKGMLEVTGPDAPDIFRAGGR"
     gene            complement(2969..3715)
                     /locus_tag="VF276_16490"
     CDS             complement(2969..3715)
                     /locus_tag="VF276_16490"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HEX6608502.1"
                     /translation="MSQISEAIHNHHQEMMNTLTNHVTAIVEGLPEADPRGLVTFLTG
                     DLLPHAVGEEQHMYPAVDPLVRTYGRPTATMTVDHEFIQEYIRHIQETVHELETATPE
                     TRPAVQARLNRLSLKIEAIFQLHLEKEERVYIPLFEKYLPVAEQQRILDGMHETYDNA
                     APAAEAVTLDVRPMPPARRHSTIFETFAQLDPGAAFVLVNDHDPKPLYYQFKVEHAGE
                     FTWDYLEQGPEVWRVRIGKAGAAEAVAAAH"
     gene            complement(3761..4114)
                     /locus_tag="VF276_16495"
     CDS             complement(3761..4114)
                     /locus_tag="VF276_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015717619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEX6608503.1"
                     /translation="MKIAPTLLDTPDLLAADPAHQQALGCLYDVIDPELGINIVDLGL
                     VYNLDVDGGLVRVEMTMTTPACPLHDYLTEQVEQAIRGEMPDVRTVAVHLVWDPPWGP
                     ERMSRAARQDLGWPD"
     gene            complement(4111..4335)
                     /locus_tag="VF276_16500"
     CDS             complement(4111..4335)
                     /locus_tag="VF276_16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEX6608504.1"
                     /translation="MDQTSLTTDLTIADVLARWPQTVPVFLRYRLACVGCPIAPFEQL
                     ADIASNYGLPLDGLLAALQQAIQPSKEPAP"
     gene            complement(4419..5162)
                     /locus_tag="VF276_16505"
     CDS             complement(4419..5162)
                     /locus_tag="VF276_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX6608505.1"
                     /translation="MSTASPVPEDLTYLHKARLFRDLPESTLREIVRVGRRRRVAADG
                     FFFRQGRPAAVLFVLLAGQVKVTQITPEGQQIILRVVGPGEMFGAVAALGEATYPASA
                     QAADACIAIGWDSATITDLMERFPHLTLNALGIVVARVHEFQDRYRELATERVERRVA
                     RALLRLVPQVGRRVEGGVLLDLALSRQDLAEMTGTTLFTVSRILSRWEQEGLIESGRE
                     RVVLRRPHSLVEIAEDLPPGGPAAPDPAK"
     gene            5235..5699
                     /locus_tag="VF276_16510"
     CDS             5235..5699
                     /locus_tag="VF276_16510"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX6608506.1"
                     /translation="MKRHPSLRDLSSDHHQGLVQARRLVQTATGTVPPPPDGAAGVVH
                     DFLHLWAEETNHHFREEEEVLLPAFARYGDPAAAPVVRMLVEHVQIRRLVADLQAQLA
                     AGAPDPATMQATGDLLREHIRHEENVVFPLIEAAMPEEALAALEQQLLQFTS"
     gene            5774..>6041
                     /locus_tag="VF276_16515"
     CDS             5774..>6041
                     /locus_tag="VF276_16515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX6608507.1"
                     /translation="MPSTIPLGRVGPLTIRLNYSWLPGALLTLWWCALIWLPAQYPDW
                     TGIFYWIVAVAVILVYLLSTIVHELAHTVVAGLGRHPLHLYPFGA"
BASE COUNT          966 a         1994 c         2189 g          892 t
ORIGIN      
        1 gggcgcgggg ctgatgctga tcggggcggc gcacgaccac cggctggcgg cggtcgtcgg
       61 cgacagcacc tacccggtcg tcgggccgat gttcgggcag tggaacagcg tcgggctacg
      121 catctggccc taccggctga gcttcgcgcc gctggccgag cccacggcca acctgttgct
      181 cgacagtcgc ctggccgaac tcgacccgct ggagcaggcg cctaacgtca gccccagcgc
      241 gctactgctg atccacgccg agcacgacca caacgggctg acgccgctca gcggagcgcg
      301 cgagatcttc gccgccgccc gcgagccgaa ggcgctctgg atctcgccca tcggcgacca
      361 cgcggttatc gcgacggcga acccggacgc ctaccgggcg caggtagtgg gcttcttcga
      421 ccactaccta cgcggccggt agggataccg ggcggggccg tttggtccgg ccccggcgca
      481 cggccaccgc ggtgctgccg atgaagaaca acagcaccag cacgttgagc agcccgcccc
      541 actgccgccc cggcagccag ccggtcaggt ccgaccccac gcgcagcagc agcgacaggt
      601 gcagcaggat gagatgcgcg tagaagacgc ggtggaaggg cacagccagg ccggtgacgg
      661 cgggcaagat gatcggcgcg tgggcgaaga tcatggcgaa gacgtagccg aggaagacgg
      721 cgtgcagtag ggcgtcgtag cgcggtccgg cggccacgcc gccgaaaacc acggccatga
      781 ggcccgccgc ggtcagccag acatagccgg agagcaggca gacggcgctg aagcgcggaa
      841 ggccgggtag ccgcaccgtg cgccgggcca cgtcgtagcg ggccagccag gcggccaggg
      901 ccaccatgcc cgcgcccgcc agccgcgcgc ccgcgtcgta gacgacgatg ctcagggcca
      961 gtccggccag gaacagcccg gcggcggcca agaagagcgc ctggctcgcc cggctggtgc
     1021 ggcgcaggcg gctcagttcc aaccgctcgc ccgcgatggt cagcacgagg aagccggccc
     1081 accagagcac gatctggtgg atgggccggc ccactgccca gagtgcgttg ccgatggccc
     1141 aggcgtcggc gccgagggcc atggtcacgg tgaacagcgc cacctggcgg cgcaggatcg
     1201 cggtgtagtt ggcgagcaag gccacgctgc ccgcgaccag cagccccgcg ccgagcggta
     1261 ccggcacgcc gatgagcagg acgacggctc ccgcggcggt gaacagcggc gccagatagg
     1321 cccagggccg gcccagcgcc acggcgcgct cgacgccgat gagcgtgccc aggaagccgc
     1381 ccaccatcag cgggccgtgg gcggcggcca gcgtgtcgcg caggggcggc aaggcccagc
     1441 ccaggcgcag cagtcccgcc cacatcgccg ccagcagggc caccatgccc acggccagaa
     1501 tgggcaggcg cgccttcggg ggaatccggt cgggcatggc tagcggcctc ccgcgcggaa
     1561 gatatcgggc gcgtcggggc cggtgacttc gagcatcccc ttcagccccc gctcggcccg
     1621 cgacagggcg tgatccatga acatgtagcg ccccggcacg tgcagcccga actcgaccat
     1681 ggccgagccg ccgggcggca ccagcaccgc ctgcacattg gtcagcggcg gcgactccag
     1741 cgcgcccagg ttgtagaccc ggtcgaatac cgcgccgatg acatggaacg acgaaggcag
     1801 gttcggcccc gcgtcgccga tgaagatgcg caccgtctgg cccacctgcg cctgcaaggg
     1861 atgctggtcg ctgagcgcgc ccaccgcgcc gttgaacaca aagtacgtgg gctgctcggc
     1921 cagcaaccgg tcggtgctca ggttctgccg gcccggcgcg ccgaagggct ggtcggtata
     1981 gagttcgctc tgcatcacgt agaactcgcg gtccaccggc ggcagcccgc cttcgggctc
     2041 gaccaggatc atgccgaaca tgccgctcgc caggtggctg gcgaccatgg gcgtggcgca
     2101 gtggtagacg aagaggccgg ggtagagcgc cttgaacgtg aacgcctttt cctggccggg
     2161 tgccacctgc atcagcccgg cgccgccgtc gggtccggcc acggcgtgca ggtcgatgga
     2221 gtggaccatg tggctgtcgg cgcggttctt caggtagacc gtgaccgtat cacccacgcg
     2281 cacgcgcagg aaggggccgg gcacctggcc gttaaacgtc cagtaggtat aggtggtgcc
     2341 ggacgccagt tggccctcca gttccacggc ttcgaggtgg atctccacgg cctgcgggcc
     2401 gcgcctggcg agcggcggcg gcaggtcggt ggggtcgcgg acgatgctcg gagcgtgggc
     2461 gacctgcggc gtggccgccg ggccgacggc gaggcggcct tccatgccgc tcgcgcggtg
     2521 gccgggcagg gtgcagaagt aggggaaggt accgggcttg tccgcggtga actgcagcgt
     2581 ggtctgggcg ccggtggccc gcaggcgcgc cgaggcagcc tggaagtcgg gcaccacgag
     2641 gtcgtgctca cccgcgtcgc cgttgatcag cgtgatcgcc acggcggcgc cggcgggcac
     2701 ttcgagcgtc gggttgacca ccttgtcaat cgccccgccc acgccgatgt agaccatgcg
     2761 cccgccgtca aggccggtgc gcagggtgaa agcggcaggg cccgccgtgc tcgcgggcat
     2821 ggcggccatg tcatggtcgc cgggggcggc gggggtgggg acgggcatgg ccggtgtgcc
     2881 ggtggcggtt gccaccagca gggcaaccag caccggcacg gccagggccg cgaagatgag
     2941 gacggacttc gacgcctgga tcatcgcgct aatgggcggc ggcgacggcc tcggcggcgc
     3001 cggccttgcc gatgcgcacg cgccagactt ccggcccctg ctccaggtag tcccaggtga
     3061 actcgcccgc gtgctcgacc ttgaactggt agtagagtgg cttgggatcg tggtcgttga
     3121 ccaggacgaa ggccgcgccg gggtcgagtt gggcgaaggt ctcgaagatg gtgctgtggc
     3181 ggcgggcggg cggcatgggg cgcacgtcga gcgtgaccgc ttcggcggcg ggcgcggcgt
     3241 tgtcgtaggt ctcgtgcatg ccgtcgagga tgcgctgctg ctcggcgacg ggcaggtact
     3301 tctcgaacag cgggatgtag acgcgctctt ccttctccag gtgcaactgg aagatggcct
     3361 cgatcttcag gctcagacgg ttcaggcggg cctgcacggc gggccgggtc tcaggcgtgg
     3421 ccgtctccag ttcgtggacg gtctcctgga tatggcggat gtattcctgg atgaactcgt
     3481 ggtccacggt catggtggca gttggccggc cataggtgcg caccagcgga tcgaccgccg
     3541 ggtacatgtg ctgctcctcg cccacggcgt ggggcagcag gtcgccggtc aggaaggtga
     3601 cgaggccgcg cgggtcggcc tccggcagac cctcgacaat cgcggtgacg tggttggtca
     3661 gggtgttcat catctcctga tggtggttgt gaatggcttc gctaatctgg ctcatgatcg
     3721 agatctcctt aggttggttg aatggatggc gaaatacggt ttaatcgggc cagcccagat
     3781 cctggcgggc ggcgcgcgac atgcgctccg gcccccaggg cgggtcccac accaggtgca
     3841 ccgcgacggt gcgcacatcg ggcatctcgc cgcggatcgc ttgctcgacc tgctcggtca
     3901 ggtagtcgtg cagcgggcag gcgggcgtgg tcatggtcat ctcgacgcgc accagcccgc
     3961 cgtccacatc caggttgtag accaggccga ggtcgacgat gttgatgccc agttcgggat
     4021 cgatcacgtc gtagaggcag ccgagggctt gctgatgggc gggatcggcg gccagcaggt
     4081 cgggcgtgtc gagcagggtc ggggcgattt tcatggtgca ggttccttcg agggctggat
     4141 agcctgttgc agcgccgcga gcagcccgtc gaggggcagg ccgtagttcg acgcgatgtc
     4201 ggcgagttgc tcgaaagggg cgatcgggca gcccacgcag gccaggcgat agcgcaggaa
     4261 cacgggcacg gtctgcggcc agcgcgccag cacgtcggca atagttaaat cggtggtgag
     4321 tgatgtttga tccatggcgc atccctttct gtatatagga tacgccatcg ggcggggcgg
     4381 gtgtttgcgc tagcgcaaac aagccgccaa gtggccgatc attttgcggg atcgggtgcg
     4441 gcggggccgc cgggcggcag atcttcggcg atctcgacca ggctatgcgg gcggcgcagc
     4501 accacgcgct cgcgcccgct ctcgatcagg ccctcctgct cccagcggct gaggatgcgg
     4561 ctcacggtga acagcgtggt gccggtcatc tcggccaggt cctggcgcga gagggccagg
     4621 tcgagcagca cgccgccctc gacgcggcgg cccacctggg gcaccaggcg cagcagggcg
     4681 cgggccacgc ggcgctcgac tcgctcggta gccagttcgc ggtagcggtc ctggaactcg
     4741 tgcacgcgcg cgacgacaat gcccagcgcg ttgagcgtga ggtgggggaa gcgctccatc
     4801 aggtcggtga tcgtggcgct gtcccaaccg atcgctatac aggcatcggc ggcctgggcc
     4861 gaggcggggt aggtggcctc gcccagggcg gccaccgcgc cgaacatctc gccgggaccg
     4921 accacgcgca agatgatctg ctggccctcc ggcgtaatct gcgtcacctt gacctgcccg
     4981 gccagcaaca cgaagagcac cgcggcgggc cggccctggc ggaagaaaaa gccatcggcg
     5041 gccacccggc gccgccgccc cacccgcacg atctcgcgca gcgtggactc cggcaaatcg
     5101 cgaaagaggc gcgccttgtg caaataggtc aggtcttccg gtacaggact tgcagtgctc
     5161 atgctataga ttgtacacga tatacccgcg ggctaacacc tgcgtcccag cggctggaag
     5221 gagacccaac attcatgaaa cggcacccta gtttgcgcga tctgtcgagc gatcatcacc
     5281 agggcctggt ccaggcccgg cggctggtgc agacggcgac cggcaccgtg ccgcccccgc
     5341 cggacggcgc ggcgggcgtg gtccacgact tcttgcacct gtgggcggag gagaccaacc
     5401 accactttcg cgaggaagaa gaggtgctgc tgcccgcgtt cgcgcgctac ggcgaccccg
     5461 cggcggcccc ggtggtgcgc atgctcgtcg aacacgtgca gatccgccgc ctggtcgccg
     5521 acttgcaggc ccaactggcc gcaggcgcgc ccgaccccgc gacgatgcag gccaccggcg
     5581 atctgctgcg cgagcacatc cgccacgagg agaacgtcgt gttcccgctg atcgaagcgg
     5641 caatgcccga agaggcactg gccgcgctgg agcagcaact tctccagttc acgtcgtgat
     5701 acgatatatc tggtatgttc cttgctatgg aatagggtag agacttgccc gtccgtagta
     5761 aggagaaacg acaatgccgt cgaccatccc cctgggccgt gtcggcccac tgaccatccg
     5821 tttgaactat agctggctgc cgggcgcctt gctcaccctc tggtggtgcg ccttgatctg
     5881 gttaccggcg caatatcccg attggaccgg catcttctac tggatcgtcg ccgtggcggt
     5941 gatcctggtc tacctgctca gcaccatcgt gcatgagctg gcgcataccg tggtggccgg
     6001 gctggggcgg caccccctcc acctgtaccc gttcggggcg g
//
LOCUS       DASUYC010001010         2275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1323919, whole genome shotgun sequence.
ACCESSION   DASUYC010001010 DASUYC010000000
VERSION     DASUYC010001010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1323919"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="VF276_16520"
     CDS             <1..1084
                     /locus_tag="VF276_16520"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX6608508.1"
                     /translation="AWQTFVGYLTLQTPPASAFNPFDALQQIAYGAVVFLLAPFLILT
                     GAAQSPAIDARFPRYVKLFGGRQGARSLHFIGLVAFLLFIVIHVTMVVVTGFGENMGN
                     IIFGQHDHDQGLAIAIGLGIIAVSIIIWALTSAASRGAPRTWQHVLGAIIRPGLRTLS
                     YRLRSRQTYPQDEISPSMILNGYPPESEDYEREVAGNFKDWALEVRGLVQQPLHLTMA
                     DIEALPKQTQITKHQCIQGWTGIAEWGGVPLSEILNRCRPLPGARFMMFISEQLDAKG
                     RPFHESLPIEMARLPQTILAYEMNYQPLSVPHGAPLRLRVETQVGFKMVKWLRTIEFV
                     ASYSGYGEGQGGSREDIQQFEPVVAV"
     gene            complement(1121..>2275)
                     /locus_tag="VF276_16525"
     CDS             complement(1121..>2275)
                     /locus_tag="VF276_16525"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6608509.1"
                     /translation="LIGPLTNERSFAGAIVLPFVGRMSDGAHTRLGKRRPFFLVGIPL
                     MAAALVLLGLHPPFWIMLTTVLLAGGFLFIALGPYQALMADITPEGQRGQVGGFMALA
                     GMVGAVVVSVIASFFWDRHAEWVFFATAGVLVLSFGVTFLMVREPAHHFAPPTRMRAP
                     AILLAAPRLRPLPPPPKLPPLDYLRDLLRYKQTGRYIGAMSLYWMAAGGATPFITLFG
                     TQTLHLDGNQASQLFLVLVLSTAAGTVFVTLLADRLGKKRVLHAGLGLFVLAALAASA
                     VQDVGQAIPVMVLAGLGNACPTALGLALLIDLIPHDRGGEFIGVASFVWSVVQPLGSF
                     FSGLLVDVMHSDRWAFGFAAVMMSLAVAALFFVHPAAALAAAEREPVPGP"
BASE COUNT          412 a          773 c          774 g          316 t
ORIGIN      
        1 agcctggcag accttcgtcg gctacctgac gctgcaaacg ccgcccgcga gcgcgtttaa
       61 tccgttcgat gcgctgcaac agatcgctta cggggccgtg gtcttcctgc tggcgccctt
      121 cctgattctg accggcgccg cgcagtcgcc ggccatcgat gcgcgcttcc cccggtacgt
      181 caagctcttc ggggggcggc agggcgcgcg cagcctgcac ttcatcggcc tggtggcctt
      241 cctgctcttt atcgtgatcc atgtgacgat ggtggtggtc acgggcttcg gcgagaacat
      301 gggcaacatc atcttcgggc aacatgacca cgaccagggc ctcgccatcg ccatcgggct
      361 gggcattatc gccgtcagca taatcatctg ggcgctgacc tccgcggcct cgcggggcgc
      421 gccgcggacc tggcagcacg tcctgggcgc cattatccgg ccggggctgc gcacgctctc
      481 ctaccgcctg cgctcgcgcc agacctaccc gcaggatgag atttccccga gcatgattct
      541 gaacggctat ccgccggagt cggaagacta cgagcgcgag gtggccggga acttcaagga
      601 ctgggcgctg gaggtgcgcg ggttggtcca gcagccgtta catctgacca tggccgacat
      661 tgaagcgctg cccaagcaga cccagatcac caagcaccaa tgcatccagg gctggacggg
      721 gatcgccgag tggggcgggg tgccgctgag cgagatcctg aaccgctgcc ggcccctgcc
      781 gggcgcgcgc ttcatgatgt tcatctcaga acagttggac gcgaagggcc gcccgttcca
      841 cgagtcgctg cccatcgaga tggcacgcct gccgcaaacc atcctggcct atgaaatgaa
      901 ctaccagccg ctctcggtgc cccatggcgc gccgttgcgg ctgcgcgtgg agacgcaggt
      961 ggggttcaag atggtgaagt ggctgcgcac gatcgagttt gtcgccagct acagcggcta
     1021 cggcgaaggc cagggcgggt cgcgcgagga catccagcag ttcgagccgg tcgtcgccgt
     1081 ctgatccagg gccggcgcgg gccgggcccc cggcccctgc tcaggggccg ggaaccggtt
     1141 cgcgctccgc ggcggccagc gccgcggccg gatggacgaa aaagagcgcg gccacggcca
     1201 ggctcatcat cacggcggca aaaccgaaag cccagcgatc cgagtgcatc acgtcgacca
     1261 gcaagcccga gaagaacgac cccagcggct gcaccaccga ccagacaaac gaggccacgc
     1321 cgataaactc gccgccgcgg tcgtggggga tcaggtcgat caacagggcc aggccgagcg
     1381 ccgtcgggca ggcgttgccc agtccggcca gcaccatgac cgggatggcc tgccccacgt
     1441 cctgcacggc ggaggcggcc agcgcggcca gcacgaacag gcctaacccg gcgtgcaaca
     1501 cccgcttctt gcccagccgg tcggccagca gcgtaacgaa gaccgtgccc gcggcggtgc
     1561 tcaacaccag cacgagaaac aactggctgg cctggttgcc gtcgaggtgc aaggtctgcg
     1621 tgccgaagag cgtgatgaac ggggtggcgc cgcccgccgc catccagtag aggctcatcg
     1681 cgccgatata gcggccggtc tgtttatagc gcaggaggtc gcgcagataa tcgagcggtg
     1741 gcagtttggg cggcgggggc aagggccgca gtcgcggcgc ggccagcaag atcgccggcg
     1801 cgcgcatccg cgttgggggc gcgaaatgat gggccggctc gcgcaccatg aggaaggtga
     1861 cgccgaagct gagcaccagc acgcccgccg tggcgaaaaa cacccactcg gcgtgccggt
     1921 cccagaagaa cgaggcgatg acggagacga ccacggcgcc caccatgccg gccagggcca
     1981 tgaacccgcc cacctggccc cgctggcctt cgggcgtgat atcggccatc agcgcctggt
     2041 aggggccgag ggcgatgaac aggaagcccc cggccagcag caccgtggtg agcatgatcc
     2101 agaacggcgg atgcagcccc agcagcacca gcgccgccgc catcagcggg atgccgacca
     2161 ggaagaaggg ccggcgcttg cccagccggg tgtgcgctcc atcgctcatg cgccccacga
     2221 acggcaagac gatcgccccg gcgaacgagc gctcattggt cagcgggccg atcag
//
LOCUS       DASUYC010001011         4578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1861564, whole genome shotgun sequence.
ACCESSION   DASUYC010001011 DASUYC010000000
VERSION     DASUYC010001011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4578
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1861564"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="VF276_16530"
     CDS             <1..814
                     /locus_tag="VF276_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cysteinyl-tRNA synthetase"
                     /protein_id="HEX6608510.1"
                     /translation="AQGLIALFGSGETSSQGRKIHDYLLSRLPVPVQVAILETPAGFQ
                     PNAEGVNLKIQQFLEKSLQNFKPQIELVAARRKDSAHDPNDPATVAPLERADYIFAGP
                     GSPTYAVRHLRDSLAWRMMRARWEAGATLAFASAAAISIGTRVLPVYEIYKCGADLGW
                     ETGLALLSDFDLTIMPHWNNAEGGAGLDTSHCFMGLERFEELYAMLPPATTVLGIDEH
                     TACIIDPAAGQCRVLGAGGVTIQHGGETQVFASQTTFPLDLLAGTALAAERG"
     gene            988..1063
                     /locus_tag="VF276_16535"
     tRNA            988..1063
                     /locus_tag="VF276_16535"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1021..1023,aa:Ala,seq:ggc)
     gene            complement(1688..2926)
                     /locus_tag="VF276_16540"
     CDS             complement(1688..2926)
                     /locus_tag="VF276_16540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608511.1"
                     /translation="MAQTTATGTTLARPAGQVARSRLQSPYAVTALVGLLYLGLLAWL
                     WQHFHGDPLGFVHIGTIWHDHNPAGTWGYDGQFYYYTAVYPSHAADFMDLPAIRLQRI
                     FFPLIIFVFALGQDALVPYVIPVLNWLAVVGGTLLVALLLQRWGRSPWFALSYALASG
                     IPVALTFDTAEPLTFALVALGVLLWEYHRPAEAADRPTGPAPRRALLAAGLTFAAALL
                     TRELAAYFVAAYAAASVLRRDWRGFLVAALAFVPVTLWGLYLSASLHTLSVSQVQPFE
                     HIPFITYWIQGNLVDPQWRSAGYAAQYIVPAFVFGVLGLAYFARPFVAWLRTRTWQPP
                     SPLLIAVLGNVHLMTFLPRLGYQHQVAVSRYALGLALAAVLWAGAARPRWLLWLTPLC
                     ALSILAYLYGLVTQDPAYLW"
     gene            3079..>4578
                     /gene="leuS"
                     /locus_tag="VF276_16545"
     CDS             3079..>4578
                     /gene="leuS"
                     /locus_tag="VF276_16545"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX6608512.1"
                     /translation="MESRYRPQGIEPKWQQFWQDAQLYYTNARPDKPKFYTLVMYPYP
                     SGDLHMGHMRNYAIGDVIARYHTMLGYDVMNPMGWDAFGLPAENAAIKEGLHPQDRTL
                     ANIARMKEQFFKMGIVYDWPREVNSCLPDYYRWTQWLFLLMYQRGLAYKKLAAVNWCP
                     NDQTVLANEQVVNGRCERCGAIVTRRDLNQWFFKITDYAQRLLDDLDTLDEWPERVRV
                     MQRNWIGRSEGAELAWPVAGRPETIRFFTTRPDTVYGATFMVLAPEHPLVPTLTRPEQ
                     QAAVDDYIEQARRETEIERLATDKEKTGVFLGSYVVNPFTGKEIPIYIADYVLVTYGT
                     GAIMAVPGGDERDYDFAQKYGLEIIPVVAPPTGAETAYIAAGAPAAEPVAGPVVKGDR
                     DNPPLYTGPGTMINSGPLDGLPTAEAKREVIATVEHEGTGRGTVNFRLRDWLISRQRY
                     WGPPVPMVYCDGCGEVPVPADQLPVLLPYDVDFRPSGESPLARNDAFVHT"
BASE COUNT          845 a         1481 c         1591 g          661 t
ORIGIN      
        1 ggcccaaggc ttaatcgcgt tgtttggatc gggcgagacc tcgtcccagg ggcgcaagat
       61 ccacgattac ctgttatcgc gcctgcccgt gcccgtgcag gtggcgattc tcgaaacccc
      121 tgccgggttc cagcccaacg ccgaaggcgt gaacctcaaa atccagcagt ttctcgaaaa
      181 gagcctgcaa aacttcaagc cgcagatcga actcgtggcg gcccggcgca aggacagcgc
      241 tcacgacccc aacgacccgg ccacggtcgc gcctctggag cgcgccgatt acattttcgc
      301 ggggccgggc agccccacct atgccgtccg ccatttgcgc gactcgctgg cctggcgcat
      361 gatgcgcgcc cgctgggaag ccggggccac gctcgctttc gccagcgcgg cagccatctc
      421 catcggcaca cgggtgctgc cggtctatga gatctacaaa tgcggggccg atctcggttg
      481 ggagacgggg ctggcgttgc tctcggattt cgatctcacg atcatgcccc actggaacaa
      541 cgccgagggc ggggccggac tcgacacgag ccactgcttt atgggcctgg aacgcttcga
      601 ggagttgtac gccatgttgc ccccggccac cacggtgctg ggcatcgacg agcatacggc
      661 ctgtatcatc gacccggctg ccgggcagtg ccgggtgctg ggcgcgggcg gggtcacgat
      721 ccagcacggc ggcgaaaccc aggtcttcgc ctcgcagacc accttcccgc tcgatctgct
      781 ggccgggacc gccctggccg ccgagcgcgg ctgacctaac cccccttgcc ccccttcccg
      841 cgccgggaag ggggagactc gacggcggaa ggcggcgcgc gcgaccgaga gcgcgccgtg
      901 ggcggcgaat ccgccgaaag atggagccaa ctaccgggct gggcggcttg catttccggc
      961 acaggtttgt tataatgcag cagtcacggg gacgtagctc agctgggaga gcacgacact
     1021 ggcagtgtcg gggtcagggg ttcgagcccc ctcgtctcca ccaaggcaaa cccgaccagg
     1081 ctcctatcca gggagcctgt cgcatatata gcaggctgtg aacaggagca gtaagccgtt
     1141 ccacccacag cgagcagggg atggtggaag ccctgcatct ggagggtccg ccccggcggg
     1201 ccgtaaggct gaactcacct gggagccgca ccggtgaacg tgccggatcg ccaggcgggc
     1261 cgcgaggccg ccggagatcc gccctgagcc gggccgggta cgcccgttag cgcgtctcaa
     1321 gtgggttcgc cgcgcctgcg ggtgagcggc ggccaacatg ggtggtaccg cgtgaggtcc
     1381 tgtgcctctc gtcccttgtc gcgcattgcg cctgggacga gaggtttttt atttgcccgc
     1441 cgccatcgtg cgccggcgtt cctaagttca ccgcgaagga acgaaggacg cgaagacgat
     1501 tgatgtaagg tttgtccttc gcgccgttca cggcgcagaa gcagggtaga tgacaggatg
     1561 gtagtgtcac aggcgcgggc cggtttgaaa ccggccccta caccggcaag acttttagca
     1621 cgttcaaaac catttgatca agcaggagtt cccggccgtc tgacccctga cggctgacgg
     1681 ctgacggcta ccagagatac gccgggtcct gcgtgaccag gccgtaaagg taggccagga
     1741 tgctgagcgc gcagaggggc gtcagccaga gcagccagcg cgggcgggcc gcccccgccc
     1801 agagcacggc ggccagggcc aggcccagcg cgtagcggct gacggccacc tggtgctggt
     1861 agcccaggcg cggcaggaac gtcatcagat ggacgttgcc gagcacggcg atcagcaggg
     1921 gcgagggcgg ctgccaggtg cgggtgcgca gccaggcgac gaaggggcgc gcgaagtacg
     1981 cgagaccaag cacgccgaac acgaacgcgg gcacgatgta ctgggcggca tacccggcgg
     2041 accgccattg cggatcgacc agattgccct ggatccagta ggtgataaac gggatgtgct
     2101 cgaacggctg cacctgcgac acgctgagcg tgtgcaggct ggcgctgagg tagaggcccc
     2161 agagcgtcac cggcacgaag gccagcgcgg cgaccaggaa gccccgccag tcgcggcgca
     2221 gcacggaggc cgccgcataa gcggccacga agtaggcggc cagttcgcgg gtcagcagcg
     2281 cggcggcgaa agtgaggccc gcggccagca gcgcccggcg cggcgccggc ccggtgggcc
     2341 gatcggcggc ttcggcgggg cggtggtact cccagaggag cacccccagc gccaccagcg
     2401 cgaaggtcag cggctcggcg gtgtcgaagg tcagggccac ggggatgccg gaggcgagcg
     2461 cgtagctcag ggcgaaccag gggctgcggc cccagcgttg cagcagcagc gccaccagga
     2521 gcgtgccgcc gaccacggcc agccagttga ggaccgggat gacgtagggt accagcgcgt
     2581 cctggcccaa cgcgaagacg aaaatgatga gcgggaagaa gatgcgttgc aggcggatgg
     2641 ccggcaggtc catgaagtcg gcggcgtgcg agggatagac ggcggtgtaa tagtagaact
     2701 ggccgtcgta gccccaggtc ccggcggggt tatggtcgtg ccagatggtg ccgatatgga
     2761 cgaagccgag cgggtcgccg tggaagtgct gccagagcca ggccagcagg cccaggtaaa
     2821 gcagcccaac cagcgcggtc acggcatagg gactttgcag gcgggagcgg gcaacctgcc
     2881 cggcggggcg ggccagcgtg gttccggttg cggtggtctg ggccatgaac atagcacctg
     2941 atactgacga ctaatgaact attgtaggag ccggtttgaa accggcccag gaaacctgac
     3001 gatgtgacaa agcgcacgta aatcctgatg atcggccggc gaaggcgcca ccgatccccg
     3061 gcccaaagga ggccccgtat ggagagccgc taccgtccgc aagggattga gccgaagtgg
     3121 cagcagttct ggcaggacgc tcaactatac tacacgaatg cgcggcctga caagcccaag
     3181 ttctataccc tggtcatgta cccttatccc tcgggcgatc tgcacatggg ccacatgcgc
     3241 aactacgcca tcggtgacgt gatcgcccgc tatcatacca tgctgggcta cgacgtgatg
     3301 aacccgatgg gctgggacgc cttcggcctg cccgccgaaa acgcggcgat caaggagggc
     3361 ctgcacccgc aggaccgcac actggcgaac atcgcgcgca tgaaggagca gttcttcaag
     3421 atgggcatcg tctacgactg gccccgcgaa gtgaactcct gcctgcccga ctactaccgc
     3481 tggacgcagt ggctgttcct gctgatgtac cagcgcggcc tggcctacaa gaagctggcc
     3541 gccgtcaact ggtgcccgaa cgaccagacg gtgctggcga acgagcaggt ggtcaacggc
     3601 cgctgcgaac gttgcggcgc aatcgtgacc cgccgcgacc tgaaccagtg gtttttcaag
     3661 atcaccgact acgcccagcg cctgctggac gacctggaca cgctggacga gtggccggag
     3721 cgcgtgcgcg tgatgcagcg caactggatc gggcgctccg agggcgcgga actggcctgg
     3781 ccggtggcgg gccgcccgga gacgatccgc ttcttcacca cgcgccccga cacggtgtac
     3841 ggcgcgacgt tcatggtgct ggcgccggaa cacccgctgg tgcccacgct gacccgaccc
     3901 gagcaacagg ccgctgtgga cgactatatc gagcaggcgc gccgcgagac cgagatcgag
     3961 cgcctggcga ccgacaagga gaaaaccggc gtcttcctgg ggtcctacgt ggtgaacccg
     4021 ttcaccggca aggagatccc aatctacatc gccgactacg tgctggtgac ctacggcacc
     4081 ggcgcgatca tggccgtgcc cggcggcgac gagcgcgact acgactttgc ccagaagtac
     4141 ggcctggaga tcatcccggt ggtggcgccg cccaccgggg cggagacggc ctacatcgcc
     4201 gccggggcgc ccgccgctga gccggtggcc ggcccggtgg tcaagggcga ccgggacaac
     4261 ccgccgctgt ataccggccc cggcacgatg atcaacagcg gtccgctgga cggcctgccc
     4321 accgccgagg ccaagcgcga ggtgatcgcc acggtggagc acgagggcac cgggcggggc
     4381 acagtcaact tccgcctgcg cgactggctg atcagccgcc agcgctactg ggggccgccc
     4441 gttcccatgg tctactgcga cgggtgcggc gaggtgccgg tgccggcgga ccaactgccg
     4501 gtgctgctgc cctatgatgt cgacttccgc ccgtcgggtg agagtccgct ggcgcgcaac
     4561 gatgcgttcg tccatacc
//
LOCUS       DASUYC010001012         4799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1582927, whole genome shotgun sequence.
ACCESSION   DASUYC010001012 DASUYC010000000
VERSION     DASUYC010001012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4799
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1582927"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VF276_16550"
     CDS             <1..606
                     /locus_tag="VF276_16550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608513.1"
                     /translation="TPASRNGLYVRNLNTGQDQLISVTGRNPVAADGVLLWSEERQQS
                     GRFAVAEWTLHLRKQSDPQRDSIIARDFGEFHGYHVSGDNVIWAASTPGNDKAHLYNI
                     SSGANQVVSAHAVVNPLISGNTVAWTEAPTGEIGKPPTWSIVIYNIATGAQSSIIAAS
                     ATPVQAWALMEPPRLIYSLGNAPVMGVRELYAIELPTGTAR"
     gene            complement(649..1245)
                     /locus_tag="VF276_16555"
     CDS             complement(649..1245)
                     /locus_tag="VF276_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX6608514.1"
                     /translation="MVSQPKPYYTSAEYLALERAAEYKSEYLAGEIFALAGASEQHNT
                     LTLNIAAELRTQFRGRPCRVYASDLRIKVAASGLYTYPDVVAACGELRFEDGHRDTLL
                     NPTVIFEVLSPSTEAYDRGKKFAHYRRLESLMAYVLVAQDEVHLEHYARQGSDWLLQE
                     ARGRGDTIHLPSVGGALSLSAIYENVEFSPLPAAAPLG"
     gene            1456..3015
                     /locus_tag="VF276_16560"
     CDS             1456..3015
                     /locus_tag="VF276_16560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608515.1"
                     /translation="MTTPPPPGASTILAAIVVLVLSALAAAYLLRREPRTWPVIAALA
                     TLAFGFALAYDQFLPDDSYITYRYAWNLLHGNGLVYNPGEYVLSTTTPLYTLLLAAAG
                     LVWPDLPVTSHILSLPALFAGALVLYLLLARHGKPVAGFLLAALYILNPLTAAVYSSE
                     SILHVALIFGAFYAYDQDYLEWAAALGALAVLTRGDGALVAVALGLCWLAARRWRAGW
                     RRPLRAVAIYAAISLPWYLFSWLYYGDLAPATMSAKMAQGELPGAQLFMEGFGVWWMS
                     YSAHSPGYWLLLPLALLGLLLTFPRGGDRWAWPTLLWAGLYTAGYTALGVTRYQNYYT
                     PLAPVLILLAMLGAHWLGVAVAQVVTRRAPGAPPGAAAWRQARIAGVVMLLLGIGIGI
                     GFVSTGARLYPTLPQARAVIYQQIGEWLRANTPQKASIGMLEVGTMGYYADRRVIDFY
                     GLIQPDVSEHMAHNDLAWSMQHYKPDYIAIHRETWAPTYDPWFDAHYQPLKTFEYARC
                     GCSPMVVYARK"
     gene            3002..4705
                     /locus_tag="VF276_16565"
     CDS             3002..4705
                     /locus_tag="VF276_16565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608516.1"
                     /translation="MPASSTAPVAQAQAPARPGAVAARYGLVAVLALGVLAYANALNA
                     FWVGDDFNYVVPKTLDTVLNFANPVGRAQFRPLGWISWVIDYTLYGPIPLGWHLTSLA
                     LHLLNTLLIVLLLRRLAGRWWSEDSAARTWVPLVAGAFFAVHPSHPEAVTWISARFDL
                     LAGLGTFLACWAFVHWREGGPRGFYIGTWLAAWWAMMSKESGLLVPLALLLIDLALGT
                     AQGRRLWAPGEWGALLRTAGGVVLRAGLALLAMSALLALLGRDGGRALPLVVLLAGLP
                     FPLPQRTAEHFGGGLLRLGRVHAPFLGMAAAYAGLRVGMALTGQGRLMYGAETHLQFG
                     PGTLLDSAAGYLLIGLGAWDAPAWVAGWPVALKGGLLLAALLLAGYAVRRLGRPALFA
                     ALWVPATALLTFDAAADRWFYIPSFGFCLLVAQLLYLAGRARPLLRTVPAAALILLWL
                     GLTLAANTRWVESGEVARGIVNQIRAFHPDLPRPATFYLANPPYSYNGILLFNTGFQV
                     APNFAYQDYRTIHTYIVDPPAPLPADLGPNPVFLRYEAGRMVEYPTLAALIAAQTAPS
                     P"
BASE COUNT          684 a         1722 c         1554 g          839 t
ORIGIN      
        1 actcccgcat cgcgcaacgg tctctatgtc cgcaacttga acacggggca agatcaacta
       61 atcagtgtga cgggacgcaa ccccgtcgcg gccgatggag tcttgctatg gagcgaagag
      121 cgacaacaaa gcgggcggtt cgccgtcgcg gagtggactc ttcatctacg caaacaaagc
      181 gatccgcagc gtgacagcat cattgccagg gatttcggcg agtttcacgg ctaccatgtt
      241 tccggcgaca acgtgatctg ggcagcgtcg actcccggca acgacaaagc gcacctctat
      301 aacatcagca gcggcgccaa ccaggtcgtg tccgcccatg ctgtagtgaa cccgctcatt
      361 agcggcaaca ccgtggcctg gaccgaggcg ccgaccggcg aaataggcaa accgccgacc
      421 tggtctatcg taatatataa cattgccacc ggcgcccagt catcgatcat cgccgcaagc
      481 gccacaccag tccaagcctg ggcactcatg gagccacccc ggctcatcta cagcctgggg
      541 aacgcgccgg tcatgggcgt ccgggaacta tatgcgatcg agttaccaac gggcaccgcc
      601 cgttagccgc catcgttact ccagactggc gaaacgcgga ggcttgcctc aacccagcgg
      661 tgcggcggcg ggcagcggag aaaactcgac gttctcgtag atcgcggata gggacagtgc
      721 tccgcctacg gacggcaggt gaatggtgtc gccccggccg cgggcctcct ggagcagcca
      781 gtcgctaccc tggcgcgcgt agtgctccaa atgcacctcg tcttgcgcca ccaacacata
      841 cgccatgagc gattccagcc gccggtagtg agcgaacttc ttgccccggt cgtaggcttc
      901 ggtggacggc gacagcacct cgaagatgac ggtgggattg aggagggtat cgcggtgccc
      961 atcctcaaag cgcagttccc cgcaggcggc cacgacatcc gggtaggtgt atagcccgct
     1021 ggccgctacc ttgatacgca gatcgctggc gtatacccgg cacgggcgcc cacggaattg
     1081 ggtgcgtagc tcggcggcga tattgagggt gagggtatta tgctgttcgc tggccccggc
     1141 cagggcgaag atttcacccg cgaggtattc gctcttatac tccgccgcgc gttccagggc
     1201 gaggtattcc gccgaggtgt aatagggctt gggctgcgaa accattgccg gctcctttgc
     1261 tgctcataag cgtgctccat tataccacac gcaaccgagg ctccccgccg cgcctgccga
     1321 tcctcgcaca atgccggccc tgcgccgagc gccgctctcc cccttcccgc ggcgggaagg
     1381 gggtcggcgg ggttaggttg gtcccggcac ggctgagcct gacgcctgcc ggacgttttt
     1441 gctataatag gcaggatgac gacccctcct ccgcccggcg caagtaccat cctggcggcg
     1501 attgtggtgc tggtgttgag tgccctggcc gccgcctacc tgttgcgccg cgagccgcgc
     1561 acctggccgg tgatcgccgc gctcgccacg ctcgctttcg gcttcgcgct ggcctacgac
     1621 cagttcctgc ccgacgacag ctatatcacc taccgctacg cctggaacct gctgcacggc
     1681 aacggcctgg tctacaaccc cggcgaatac gtgctgagca cgaccacgcc gctctacacg
     1741 ctgctgctgg ccgccgccgg tctcgtctgg cccgacctgc cggtgacgag ccacatcctc
     1801 agcctgcccg cgctgtttgc cggggcgctg gtgctctacc tgctgctcgc ccgccacggc
     1861 aagccggtcg ccgggttcct gctggccgcg ctttatatcc tgaacccgct caccgccgcc
     1921 gtctatagct cggagtcgat cctgcacgtg gcgctgatct tcggcgcgtt ctatgcctac
     1981 gaccaggact acctggagtg ggcagcggcc ctgggcgcgc tggccgtgct gacccgcggc
     2041 gacggggcgc tggtcgccgt cgcgctgggg ctgtgctggc tggcggcgcg gcgctggcgc
     2101 gcgggctggc ggcgccccct gcgcgccgtg gcgatctacg cggcgatcag cctgccttgg
     2161 tacttgttct cctggctcta ttacggcgac ctggcccccg ccaccatgag cgccaagatg
     2221 gcccagggcg aactgccggg cgcgcagttg ttcatggagg gcttcggcgt ctggtggatg
     2281 tcctactcgg cgcacagccc cggctactgg ctgctgttgc ccctggcgct gctgggcctg
     2341 ctgctcacct tcccgcgcgg tggcgaccgc tgggcctggc ccacactgct ctgggcgggc
     2401 ctctacacag cgggctacac ggccctgggc gtcacccgct accagaacta ctacaccccg
     2461 ctggcgcccg tgctgatcct gttggcgatg ctcggcgcac actggctggg tgtcgccgtg
     2521 gcccaggtcg tcacgcggcg cgcaccgggc gcgccaccgg gcgcggcggc gtggcggcag
     2581 gcgcgcatcg cgggcgtggt catgctgctg ctaggcatcg gcatcggcat cggcttcgtg
     2641 agcacgggcg cccgcctcta cccgacgctg ccgcaagcgc gcgccgtcat ctatcagcag
     2701 atcggcgaat ggctgcgtgc caacacgccg cagaaggcgt ccatcggcat gctcgaagtc
     2761 ggcacgatgg gctactatgc cgaccgccgc gtcatcgact tctacggcct gatccagccc
     2821 gacgtgtccg agcacatggc gcacaacgac ctggcctggt cgatgcagca ctacaagccc
     2881 gactatatcg ccatccatcg cgagacctgg gcgccgacct acgacccctg gttcgatgcc
     2941 cactaccagc cgctgaagac gttcgagtac gcccgctgcg ggtgttcgcc gatggtcgtc
     3001 tatgcccgca agtagcaccg cgcccgttgc ccaggcgcaa gctcctgccc gccccggcgc
     3061 cgtcgcggcg cgctatgggt tggtggccgt gctggccctg ggcgtcctgg cctacgccaa
     3121 cgccctgaac gccttctggg tgggcgacga tttcaactac gtcgtgccca agacgctgga
     3181 cacggtgctc aacttcgcca acccggtagg ccgtgcccag ttccgcccgc tgggctggat
     3241 tagctgggtc atcgactaca cgctctacgg ccccatcccg ctcggctggc acctgaccag
     3301 tctcgccctg cacctgctca acacgctgct gatcgtgctg ctgctacgcc ggctggctgg
     3361 ccgctggtgg tcagaggatt ccgccgcccg cacctgggtg ccgctggtag ccggggcctt
     3421 cttcgccgtc cacccctccc acccggaggc cgtgacctgg atcagcgcgc ggttcgacct
     3481 cctggccggg ctgggcacgt tcctggcctg ctgggccttc gtgcattggc gtgagggcgg
     3541 cccgcgcggc ttctacatcg ggacctggct cgccgcctgg tgggcgatga tgagcaaaga
     3601 gtcggggctg ctggtgcccc tggctctcct gctgatcgac ctggcgctgg ggacggcgca
     3661 gggccggcgg ctgtgggcgc cgggcgagtg gggcgccttg ctgcggacgg cggggggcgt
     3721 ggtgctgcgc gccgggctgg cgttgctggc gatgagcgcg ctgctggccc tgctgggccg
     3781 cgacggaggc cgcgcgctgc cgctggtggt gctgctggcc gggctgccct tcccgctccc
     3841 gcaacgcacc gcggaacact tcggcggggg gctgctacgc ctggggcggg tgcacgcgcc
     3901 gttcctgggc atggctgccg cctatgccgg gctccgcgtg ggtatggcgc tgaccggcca
     3961 ggggcggctg atgtacggcg ccgagacgca tctccagttc gggccgggca cgctgctcga
     4021 ctcggcggcg ggctacctgc tcatcggcct cggcgcgtgg gatgctcccg cgtgggtggc
     4081 cgggtggccg gtggcgctca agggggggtt gctgctggcc gcgctgctcc tggccgggta
     4141 tgccgtgcgc cggttgggcc gccccgcgct tttcgctgcc ctgtgggtgc ccgccaccgc
     4201 cctgctcacc ttcgacgccg cggcggaccg ctggttctac atcccctcgt tcggcttctg
     4261 cctgctggtg gcgcaactgc tctacctggc cgggcgcgcc cgcccgctcc tgcgtaccgt
     4321 gcccgccgcg gcgctgatcc tgctctggct cggcctcacc ctcgccgcca acacgcgctg
     4381 ggtggagtcg ggcgaggtgg cgcggggcat cgtcaaccag atccgcgcgt tccaccccga
     4441 tctgccgcgc ccggctacct tctacctggc gaacccgccc tacagctaca acggcatcct
     4501 cctgttcaac accggcttcc aggtcgcccc gaacttcgcc taccaggact accgcaccat
     4561 ccacacgtat atcgtggacc cacccgcgcc gctgcccgcc gatctcggcc ccaacccggt
     4621 gttcctgcgc tacgaagccg ggcgcatggt cgaatacccc accctggccg cgctcatcgc
     4681 cgcccagacc gcgcccagcc cctgaacccg gcgacccacc ccggccatcc tgtcataacc
     4741 ccctattctg caccgcgaag gcgcgaagga cgcgaagacc aagacgcata taaataatc
//
LOCUS       DASUYC010001013         2735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1255902, whole genome shotgun sequence.
ACCESSION   DASUYC010001013 DASUYC010000000
VERSION     DASUYC010001013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1255902"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VF276_16570"
     CDS             <1..576
                     /locus_tag="VF276_16570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two pore domain potassium channel family
                     protein"
                     /protein_id="HEX6608517.1"
                     /translation="SPPTALELLRRHCPGNDLEAIGGFMQDWERWAAELLESHLSYPV
                     LGYFRSQHDNESWLAALTMILDSCALVMAGGDGPAARTARLTFAMARHAAVDLAQVFD
                     TPPRPPARDRLPPADLAHLREALVAADVSLKTGPAVDEKLDHLRRMYEPYVNALAEHL
                     QVALPPWLPAADAHDNWRTAVWERYDGISVV"
     gene            1029..>2735
                     /locus_tag="VF276_16575"
     CDS             1029..>2735
                     /locus_tag="VF276_16575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002613218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX6608518.1"
                     /translation="MATDTPARPAPHVAPAIDILKDERHPLNAIFEPSSVAVIGASEK
                     PGSVGRTILWNLISSPFGGTVYPVNITRPSILGVKAYSDLAAIPDAVDLAVIVTPART
                     VPGIMAECVEKGVKGVIVISAGFKEIGPDGVELEQQVLAQARRGNIRVVGPNCLGVMR
                     PPAGLNATFASGIARSGSVGFISQSGALLTAVLDWSRRENVGFSAFASLGSMLDVGWG
                     DLIYYLGDDPHTRSIVIYMETIGDARSFLSAAREVALTKPIIVIKPGRTTQGSQAAAS
                     HTGSLTGSDEVLAAAFSRVGVLRVDTIAELFYMSEVLGKQPLPSGPRLTIVTNAGGPG
                     VLATDALITGGGTLAQLSAETLTALDGILPAAWSHNNPVDILGDASPERYAAALATTA
                     QDSNSDGLLVILTPQAMTDPTATAEQLRPYAQIAGKPVLASWMGGEEIEPGEGVLRAA
                     NNPNFPYPDTAARMFNYMWRYNDNLRALYETPVLPADAGSSMNREQARQIIDTVRGEG
                     RTLLTEFESKQLLAAYDIPVVETRVATSADEAVGCAEAMGYPVVLKVYSRTITHKTDV
                     GGV"
BASE COUNT          458 a          911 c          926 g          440 t
ORIGIN      
        1 tcgccgccga cagcgctgga attgctgcgc cgccactgcc ccggcaacga tctggaggcc
       61 atcgggggct ttatgcagga ctgggagcgc tgggcggcgg agttgctcga aagccacctg
      121 tcctacccgg tgctgggcta cttccgctcc cagcatgaca acgagtcctg gctggccgcg
      181 ctgacgatga tcctcgattc ctgcgccctg gtcatggcgg gcggcgacgg cccggcggcg
      241 cggacggcgc ggctcacctt tgccatggcc cgccacgccg cggtcgacct ggcgcaggtc
      301 ttcgatacgc cgccgcgccc gcccgcgcgg gatcgcctgc ccccggcgga cctggcgcac
      361 ctgcgcgagg cgctggtcgc cgcggatgta tccctcaaga ccggtcccgc cgtggacgag
      421 aaactggatc acctgcggcg catgtacgag ccgtatgtga acgcgctggc cgaacacctg
      481 caggtcgcgc tgccgccctg gctgcccgcc gccgacgccc acgacaactg gcgcaccgcg
      541 gtctgggagc gctacgacgg gatctccgtc gtgtaggacg cggtgcccgc gcgccagaga
      601 gccgatcaag tagtccccct gtgcttgacc aggggcgggg ttagccggcg cgcttggccg
      661 ttgtccctta gaacgcccaa acgggatgag tggcacacgt cttgatcagt gcctagccgt
      721 gccggccaag catttgccgc gcgggccaac caggctgcac cggctgcccg cccgattcgg
      781 gcccggatcg cgcggcgggc agcgggtcgt ctgaacggcg gggccgccgg ccttcctccc
      841 gctgggcgcg cgcatgcata ggtggaactt tgcgcccgcg ggggtttgtt aggcgcgcac
      901 cggcagtagt ttgcacagat taccagcgcg atacaaggaa cggggatcta acacatcgag
      961 aggagaatcg gccctaccgg ctagtgagca cagtcgcgtg ccactgctga gggcggtgga
     1021 acaatactat ggcaacagat acacctgcac gcccggcccc tcatgtggcg ccggccattg
     1081 atatcttgaa ggatgagcgg cacccgctga acgcgatctt cgagccgagc tcggtggccg
     1141 ttatcggcgc tagcgaaaag cccggctcgg tcggacggac catcctctgg aacctgatca
     1201 gcagcccctt cggcggcacg gtctacccgg tcaacataac cagaccgagc atcctgggcg
     1261 tgaaagccta ttcggatctg gcggcaatcc ccgacgcggt tgacctggcg gtaattgtca
     1321 ctcccgcccg taccgtgccc ggcattatgg ctgagtgcgt cgagaagggt gtcaaagggg
     1381 ttatcgtcat ctccgcgggg tttaaggaga tcggacccga cggggtagag ttggaacagc
     1441 aagtactcgc ccaggcgcgg cggggcaaca tccgggtggt ggggccgaac tgcctgggag
     1501 taatgcggcc cccggcgggg ctgaacgcca cctttgccag cggcatcgcc cgttcgggct
     1561 cggtcggctt catcagccag agtggcgccc tgctcacggc ggtgctcgac tggagccggc
     1621 gcgagaacgt cggcttctcg gcgtttgcat ccctgggctc gatgctggat gtgggctggg
     1681 gcgacctgat ctactacctg ggcgacgacc cgcacacccg ctccattgtc atttacatgg
     1741 agaccatcgg ggatgcccgc tcgttcctct ccgcggcgcg ggaggtggcg ctcaccaaac
     1801 cgattattgt gatcaagccg gggcgcacca cgcagggtag ccaggccgcc gcctcgcaca
     1861 ccgggtcgct caccggatcg gacgaggtgc tggcggcggc gttcagccgt gtcggcgtgc
     1921 tgcgcgtgga cacgatagcc gagctcttct acatgtccga ggtcctgggg aagcagcctc
     1981 tgcccagcgg cccgcgcctg acgattgtta ccaatgcggg tggaccggga gtgctggcga
     2041 ccgacgccct gatcacgggc ggggggacgc tggcccagtt atccgccgag accttgaccg
     2101 ccctggacgg catactcccg gccgcctgga gccacaacaa cccggtggat atcctggggg
     2161 atgcctcgcc ggagcgctat gccgcggcgc tcgcgacgac cgcgcaggac tccaacagcg
     2221 acggcctgct ggtgatcttg acaccccagg cgatgaccga tccgaccgcg acggcggagc
     2281 agcttcgccc ctacgcccag atcgcgggaa agcctgtgct ggcgagttgg atgggcgggg
     2341 aggagattga gccgggcgag ggagtcttgc gcgccgccaa caaccccaac ttcccctacc
     2401 ccgatacggc ggcgcgcatg ttcaattata tgtggcgcta caacgacaat ctgcgcgcgc
     2461 tctacgagac cccggtatta cccgcagacg cgggtagctc gatgaatcgc gagcaggccc
     2521 ggcaaatcat cgacacggta cgcggcgagg gccggactct cctgaccgag ttcgagtcga
     2581 agcaactgct ggcggcctac gacatcccgg tggtcgagac ccgcgtcgca acgagcgccg
     2641 atgaggccgt ggggtgcgcc gaagcgatgg gctacccggt ggtactcaag gtctactccc
     2701 gcactatcac ccacaagacc gacgtcggcg gagtg
//
LOCUS       DASUYC010001014         3425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1134686, whole genome shotgun sequence.
ACCESSION   DASUYC010001014 DASUYC010000000
VERSION     DASUYC010001014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1134686"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1423)
                     /locus_tag="VF276_16580"
     CDS             complement(<1..1423)
                     /locus_tag="VF276_16580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608519.1"
                     /translation="MPEASQNLPIRRLTLYKHGVGFVERLGSVEGTEIQLVFRADEVN
                     DALKSLLAVDLQGGQVLGIHYETPADRDARLAESGIVLAPDHSLLDLLRGLRGWQVAL
                     TVGDGATAEQYTGRLLGVEVVDGRPVSRSRVALLEAGTQSVLNLPVGGLVRIQLLEDR
                     AAHDLRFFLDTSRTEESHRTVTVRLSEGAHRLAVSYLVPSPTWRVSYRLMAERKDAAA
                     GQDGGQAEGELLLQGWGLFDNRMEEDLQEVAVTLVAGQPISFIYDLATSRIPDRPVVE
                     DEARVAAAPVEFAEMMPAPPPAPPMAAAPRMMKMAAMAAPAGGGAMAADYEAPTMGID
                     LVSGAVSTAATGADLGELFQYEVTTPVTVKRGESALVPILNARVPYRRQLLYNGAKLP
                     DHPVAALRFTNTTGLVLERGPVTVVEDGDYRGEAMVPFTKTDSEIYLAFAVELGIKVS
                     ESRDSRVEMAGLHIEGSYFKIQQA"
     gene            1637..2752
                     /locus_tag="VF276_16585"
     CDS             1637..2752
                     /locus_tag="VF276_16585"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX6608520.1"
                     /translation="MYTLTAADQTWFDQELATRREQVYAILLDPRYAAHFAPAHSREA
                     IYHYLGQRGKALRPLMLLLSCGAAGSDEARALPAAAAVELFHTWTLVHDDIIDRDALR
                     RGRPTVHAAFATRARDEWDYPAGEDAHYGVTTAILAGDQQQGWAYALLAELPERGVSP
                     ALALRLIRDLALVLEPGLLAGEMLDVQYSRRSITDLSEDLVLDMLSRKTALLYRFAAR
                     TGALIGLDATGRAGTPAGDALVAALETFAGLCGTAFQLQDDLLGITGDERLLGKPIGS
                     DLREGKKTLIIYHALNHGSPAALSRLAKVLGNAEATQAQVEDAVRVLEEIGSLNYVRS
                     MSQNYVNQAYEQLARLPDTPNRKLLYLWARYLVARES"
     gene            2760..3341
                     /locus_tag="VF276_16590"
     CDS             2760..3341
                     /locus_tag="VF276_16590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608521.1"
                     /translation="MPDSPLHKLTGAVRQFAAAFRAGPDGAGWDDPGALALRESLFAR
                     YPFTEEAKVYLRRHSHIAVRDLHSTTGGGYWDPGRQLVFLNTAQDEAALHEHAHVWWH
                     ERRLLPGQAGGMIDAVVRFSEDANPHYARIHKLAYDYVHGIPAQGWAGMLVERNDWEM
                     FAGLASGTMGDMRLLPPYVREFYVGLFQMPAGA"
BASE COUNT          519 a         1242 c         1146 g          518 t
ORIGIN      
        1 gggcctgctg gatcttgaaa tagctaccct cgatgtgcag cccggccatc tcgacccggc
       61 tgtcccgcga ctcgctgacc ttgatgccca gttcgacggc gaaggccagg tagatctcgc
      121 tgtccgtttt ggtgaagggc accatcgcct cgccccggta gtcgccgtcc tccaccaccg
      181 tgaccgggcc gcgctccagc accaggccgg tggtgttggt gaagcgcagc gccgccaccg
      241 ggtggtcggg cagcttggcg ccgttgtaga gcaactgccg ccggtagggc acacgcgcgt
      301 tcaggatcgg caccagggcg ctctcgccgc gtttgaccgt caccggcgtg gtgacttcgt
      361 actggaacaa ctcgcccagg tccgccccgg tggccgcagt ggacacagcg ccgctcacca
      421 ggtcgatgcc catggtcggc gcctcgtagt cggcggccat ggcgccgccc ccggcgggcg
      481 cggccatcgc cgccatcttc atcatgcgcg gggcggcagc cataggcggg gcgggcggcg
      541 gcgcgggcat catttcggcg aactcgaccg gtgcggcggc cacccgcgcc tcgtcttcca
      601 ccactggccg gtcggggatg cgcgaggtcg ccaggtcgta gatgaacgag atgggctgcc
      661 cggcgaccag ggtgacggcc acctcctgca agtcttcctc catgcggttg tcaaacaggc
      721 cccagccttg caacaacagt tcgccctcag cctgcccgcc gtcctgcccc gcggcggcgt
      781 ccttgcgctc ggccatcagc cggtaactca cgcgccaggt ggggctgggc accaggtagg
      841 acacggccag ccggtgcgcg ccttcgctca ggcgcacggt gacggtgcgg tgcgactcct
      901 cggtgcggct ggtgtcgagg aagaagcgca ggtcgtgggc ggcacggtct tcgagcaact
      961 ggatgcgcac cagcccgccc accggcaggt tgagcacgct ctgtgtgccg gcttcgagca
     1021 gtgccacgcg cgaccggctc acaggccgcc cgtccaccac ctcgacgccc agcaggcgcc
     1081 cggtgtattg ctctgccgtg gcgccgtcgc ccacggtcag cgccacctgc cagccgcgca
     1141 acccgcgcag caggtcgagc aggctgtggt cgggagccag tacgatgccg ctctcggcca
     1201 ggcgcgcatc gcggtcggcg ggcgtctcgt agtggatgcc cagcacctgc ccgccttgca
     1261 ggtccaccgc cagcaggctt ttcagggcgt cgttcacctc gtcggcgcgg aagaccagtt
     1321 ggatctccgt gccctcgacg ctgccaaggc gctcgacaaa gcccacgcca tgcttataga
     1381 gcgtgaggcg gcggatcggt aggttctggc tcgcttcggg catgtaaggg ttcctccgca
     1441 gggggtaagg acgggcagac atcgggcgcg gcacgcgcgc gattcagcat agcacagccc
     1501 gccaacgggt gtcaaacccg ccacgcgggg gttgcccggc ctgtgaacgg gcgggcaacg
     1561 ggtacgaagc ccgcctgcgc gggctggccc aggcccgcgt ttggcatccc gccgcgcggg
     1621 gcggtataat agcgctatgt atacattaac tgccgccgat cagacctggt ttgaccagga
     1681 actcgccacc cggcgcgagc aggtctacgc catcttgctc gatccgcggt acgcggccca
     1741 ctttgcgccg gcccattcgc gcgaggccat ctaccattac ctgggccagc ggggcaaggc
     1801 gctgcggccg ctgatgctgc tgctgagttg cggcgccgcc ggcagcgacg aggcccgcgc
     1861 cctgcccgcc gccgccgccg tcgagctctt ccacacctgg acactggtcc acgacgatat
     1921 tatcgaccgc gacgcgctgc gccggggccg gcccacggtc catgccgcct tcgccacccg
     1981 cgcccgcgac gagtgggact atcccgccgg ggaggacgcg cactacggcg tgaccacggc
     2041 gatcctggcc ggcgaccagc agcagggctg ggcctatgcc ctgctggccg agctacccga
     2101 gcgcggcgtg tcgcccgccc tggcgctgcg gctgatccgc gacctggccc tggtgctcga
     2161 accgggcctg ctggccgggg agatgctcga cgtgcaatac tcgcggcgct cgatcaccga
     2221 cctgagcgag gacctggtgc tcgacatgct cagccgcaag acggccctgc tctatcgctt
     2281 cgccgcgcgc accggcgcac tgatcgggct ggacgcgacg ggccgggccg gcacacccgc
     2341 gggcgacgcg ctggtcgcgg cgctggagac cttcgccggg ttatgcggca ccgctttcca
     2401 gttacaggac gacctgctgg gcatcaccgg cgacgagcgc ctgctgggca agccgatcgg
     2461 ctccgacctg cgcgagggca agaaaacgct gatcatctac cacgcgctga accacggctc
     2521 gcccgccgcg ctgagccggc tcgcgaaagt gctgggcaac gccgaggcga cccaggccca
     2581 ggttgaggat gccgtgcgcg tgctggagga gatcggcagc ctgaactacg tgcgctcgat
     2641 gtcgcaaaat tatgtcaacc aggcatacga gcaactcgcc cgcctgcccg ataccccgaa
     2701 ccgtaagctg ctctatctct gggcgcgcta cctggtggcg cgggaatcgt gagccaacaa
     2761 tgcctgactc gcccctgcac aagctaaccg gcgccgtgcg ccagttcgcc gcggctttcc
     2821 gcgccgggcc ggatggcgcg ggctgggacg accccggcgc cctggcgctg cgcgagtcgc
     2881 tgttcgcgcg ctaccccttc accgaggagg ccaaggtcta cctgcgccgc cacagccaca
     2941 tcgccgtgcg cgacctgcac agcaccaccg gcggcggcta ctgggacccc ggtcgccaac
     3001 tcgtcttcct gaacacggcg caggacgagg ccgcactgca cgagcacgcc cacgtctggt
     3061 ggcacgagcg gcgcctgctg cccggccagg ccggcggcat gatcgacgcc gtggtgcgct
     3121 tcagcgagga cgccaacccg cactacgccc ggatccacaa actggcctac gactacgtcc
     3181 acggcatccc cgcgcagggc tgggccggca tgctggtcga gcgcaacgac tgggagatgt
     3241 tcgccgggct agccagcggc accatgggcg acatgcgcct cctcccgccc tacgtccgcg
     3301 agttctacgt gggcctcttc cagatgcccg cgggcgcgta aatcggttct ttatagctcg
     3361 gcgggcacaa tcctagctcc cggcaccacc actttcgcct ctgccggaga gacgtagtaa
     3421 aagta
//
LOCUS       DASUYC010001015         4939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1558128, whole genome shotgun sequence.
ACCESSION   DASUYC010001015 DASUYC010000000
VERSION     DASUYC010001015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4939
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1558128"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(266..1606)
                     /locus_tag="VF276_16595"
     CDS             complement(266..1606)
                     /locus_tag="VF276_16595"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEX6608522.1"
                     /translation="MQDTPKRVLSQPGISVVLSALALGILGAGWTAIPISDSYIGGAA
                     TSLVALLLVAAIILTSQFPIHIRYYQKIDISSLVLYLIAVLLPPPLAATTAGLGVLTS
                     ELALRARRGTYLSDIVTLASRWVIIVLIGSLVASLPVGSGPAHLAVLALSGGILWIGD
                     ILTLPFVLAPISGESYGQIVKAAIADSGPAEAAQYILGLLGALAAMQHIWALALLAVP
                     GTLVYLAFKSAKEMHDGTRQLLESLADTVDLRDPYTGGHSRRVTELTEGILRELDMQG
                     AGAELIIAAARVHDIGKIGIPDRVLNKPGMLTAEERAIMETHPERGAELLRRYPDFAQ
                     GVEIVRHHHERWDGAGYPHRLKGTDIPLGARIVAVADSFDAMTSDRPYRPALPVRKAV
                     EILRTGRGEQWDAVIVDAFLRSIRPQIEQAGPSHLRLVENTEDASEPAIVKVRA"
     gene            1906..2715
                     /gene="radC"
                     /locus_tag="VF276_16600"
     CDS             1906..2715
                     /gene="radC"
                     /locus_tag="VF276_16600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEX6608523.1"
                     /translation="MSDQLYPAPPPRRKRPLTAVTLYEPDGTVTTHTETAPGPARSHT
                     TIKELPSDDRPREKLVHLGAGALSEAELLAILLRVGSQGETAIDLARRLLREFGGITG
                     LSRASVSTLCAVHGLGEAKACQLLAALETGRRLLLAEPGARLTINGPQQIGDLLVVEM
                     RAFEQEVLRVVILNTKHQVMKMVDVHRGSVNSSLVRVAEVFKEPVRANATAILVAHNH
                     PSGDPTPSRQDVEVTEQIVAAGKLLDIEVLDHLVIGHKWLSMRERGLGFAR"
     gene            2944..3696
                     /locus_tag="VF276_16605"
     CDS             2944..3696
                     /locus_tag="VF276_16605"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX6608524.1"
                     /translation="MDPTHLDRLPPAYRRAAQTILAEYSARPDVLAVLLTGSVTRGQA
                     GPMSDLDVWVLTEAPARIRSSLVVDGVPVEVFYNSPAWTARYLAQGDVSAMNMVGYGW
                     PAYIRPDAAAPVAELQARARAAYEQGPPAPTADETGRRRNLAIDTLLDAQDVLESDPL
                     AATLVLNNALEQVLRWYYAERRAWMPKLKRLPADLATRDQALAGAVRSYLVAADAPAR
                     HAALLGVFALVYPAGHGLDQWCWESTPEPFSE"
     gene            3712..4008
                     /locus_tag="VF276_16610"
     CDS             3712..4008
                     /locus_tag="VF276_16610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX6608525.1"
                     /translation="MVTALVMINAERHTVGETAQAVLKIAGVREVYSVTGEYDLVAIV
                     STPVYEQLADLVTRDITAVRTITRTHTLMAFQCYSNQDMERLWDIGSEEAEPEV"
     gene            complement(4063..4365)
                     /locus_tag="VF276_16615"
     CDS             complement(4063..4365)
                     /locus_tag="VF276_16615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608526.1"
                     /translation="MKSYYQLDPATRQRAQEHYFAVILDEMLDGRFPWGYLGSRRGMI
                     QAQVEHMRKQGDRYTQKQALAEAFRPDLRRVAQIYAQRAHYLEPGEHAVAMQDLAP"
     gene            complement(4368..>4939)
                     /locus_tag="VF276_16620"
     CDS             complement(4368..>4939)
                     /locus_tag="VF276_16620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608527.1"
                     /translation="IEHRLRLGRVLQRRQARAAGSCQIRIGARGLVEAGCYTPLVESD
                     LRPRAVQIRAGAPDVLEFALDRGQGRGRRRVLLRVPIPRGREAEAQALVHRFTTEVIA
                     AYDLRWRWLGGIPRPLRLPENVPAGTLADKFPAPLSLPAGAPTHRLTRTWVGGSPEAQ
                     RYGWGVLPAPTADGAAVPDHKTTHLPPVE"
BASE COUNT          796 a         1631 c         1707 g          805 t
ORIGIN      
        1 cgaaacctat agagttgtgg tgcgattgag aaatggtgcg tgaggttgtc ccaaccggcg
       61 aagcctggcg ccctgaccaa attcgggctt agattaggcg cgatccgggg tgggcggaac
      121 gcttcggcgg gggtagtacc ctacccagcg tgattatacg accttaccaa aggtttgtca
      181 ataggtaatg ttgatacttt ggtacgaatt ctgctttagc gagcagttta tctcagtaga
      241 gttcgccgcg ggtaccggcg aaccgtcagg cgcgaacctt gacgatagcc ggttcgcttg
      301 cgtcctcggt attctcgacc agccgcaggt gggacgggcc ggcctgctcg atttgcgggc
      361 ggatgctgcg caggaaggca tcgacgatca cggcgtccca ttgttcgccg cgcccggtgc
      421 gcaggatctc gacggctttg cgcaccggca gggcgggccg gtagggccgg tcgctggtca
      481 tggcgtcgaa gctgtcggcc acggccacga tgcgggcgcc gagggggatg tcggtgccct
      541 tgagccggtg cggatagccg gcgccgtccc agcgctcgtg gtgatggcgc acgatctcga
      601 cgccctgggc gaagtcgggg tagcgccgca gcagctccgc cccgcgctcg ggatgcgtct
      661 ccatgatcgc ccgttcctcg gcggtcagca tgccgggctt gttcaacacc cggtcgggaa
      721 taccgatctt gccgatgtcg tggacacggg cggcggcgat gatcagttcg gcgccggccc
      781 cttgcatatc cagttcgcgc aggatgccct ccgtgagttc cgtgacgcgg cgggagtggc
      841 cgccggtgta gggatcgcgc agatcgacgg tatcggccag gctttcgagc aattggcgtg
      901 tgccgtcgtg catttccttg gcgctcttga aggccagata gaccagggtg ccgggcaccg
      961 ccagcagcgc cagcgcccag atgtgctgca tggcggccag cgcgcccagc aggcccagga
     1021 tatactgcgc cgcttcggcg gggccggagt cggcgatggc ggccttcacg atctggccgt
     1081 acgattcccc gctgatcggc gccagcacaa acggcagcgt caggatgtcg ccgatccaga
     1141 ggataccccc ggagagggcc aggacggcga ggtgggccgg gccgctgccc accggcaggc
     1201 tggcgaccag gctgccgatt agcacgatga tcacccagcg cgacgccagg gtgacgatgt
     1261 cgctgaggta ggtgccgcgg cgcgcccgca acgccagctc cgacgtgagc acgccgagac
     1321 ccgcggtggt ggccgccagc ggcggcggca gcaacaccgc gatcagatag agcaccaggc
     1381 tgctgatgtc gattttctgg tagtagcgga tatggatggg gaactgtgag gtcaggatga
     1441 tggcggcgac cagcagcagg gcaaccagcg aggtggcggc gcccccgata taggagtcgc
     1501 tgatggggat cgcggtccag cccgccccca ggatgcccag ggccagcgcg ctcaggacca
     1561 cactgatccc cggctgtgat aaaacccgtt ttggcgtgtc ctgcatcacc caggtccttt
     1621 acgaggggcc tgccgggccg ggcgatctgc gaccccccgg tgttctgtgc ttgccgctca
     1681 taagcagttg tggcgcgcgc ccgcgccggt gggagtgcat tacacgtccc tgttgggcca
     1741 aaggtttatg cctggtctta tcgtagcccc gaatgtccca cctgtcaata ggaaattcgg
     1801 gctaccgcag gtcctatagg ccacgaaccc gtctattata ccagatccgg cggcctgtgc
     1861 tataatacgg ccaaccctgc ccaagagagg aacgtggccc cgcgcatgtc cgaccaactc
     1921 tatccggccc cgccgccccg ccgcaagcgc ccgctcaccg cggtgacgct ctacgagccg
     1981 gacggcaccg tgaccacaca tactgagacg gcgccggggc ccgcccggtc acacaccacg
     2041 atcaaggaac tgcccagcga cgaccgcccg cgcgaaaagc tggtccacct gggggccggg
     2101 gcgctcagcg aggccgagtt gctggcgatc ctgctgcgcg tgggcagcca gggcgaaacg
     2161 gccatcgacc tggcccggcg gttgctgcgc gagttcggcg gcatcaccgg cttgagccgc
     2221 gcgtcggtct cgacgctgtg tgcggtccac gggctgggcg aggccaaagc ctgccagttg
     2281 ctggccgcgc tggagaccgg gcgccgcctg ttgctggccg agccgggcgc ccgcctgacg
     2341 attaacggcc cccagcagat cggcgacctg ctcgtggtcg agatgcgcgc cttcgagcag
     2401 gaagtgctgc gcgtggtgat cctgaacacc aagcaccagg tgatgaagat ggtcgacgtg
     2461 catcgcggca gcgtgaacag cagcctggtg cgcgtggccg aggttttcaa ggagcccgtg
     2521 cgagccaacg cgacggccat cctggtggcc cacaaccacc ccagcggcga tcccacgccc
     2581 tcgcgccagg atgtcgaagt gacggaacag atcgtagccg ccggcaagct gctcgacatc
     2641 gaggtgctcg atcacctggt catcggccac aaatggctca gtatgcgcga gcgcggcctc
     2701 ggcttcgccc gctgacccgg catctctttg tgttcaccgc agagacgcag agaacgcaga
     2761 gatcattaat ggaagtatta tttgggggat ttgtgtagcg agatggtagt tcaccctacc
     2821 tcttgcctgc attccatctc gttctctgcg ttctctgcgt ctctgcggtg ccaatccgta
     2881 tcctaaccgc atgacaggcc cggttggttg acaggcgcgc ggggcggggg cataataccg
     2941 gccatggacc ccacccacct ggaccggctg ccgcccgcgt accgccgcgc cgcgcagacc
     3001 atcctggccg aatatagtgc ccgccccgac gtgctggccg tgctgctgac cggctcggtc
     3061 acgcgcgggc aggccgggcc gatgagcgat ctcgacgtgt gggtgctgac cgaggccccg
     3121 gcgcgcatcc ggagcagcct ggtggtggac ggcgtgccgg tcgaggtgtt ctacaattcg
     3181 cctgcctgga ccgcgcgcta cctggcgcaa ggcgatgtct cggccatgaa catggtcggc
     3241 tacggctggc cggcgtatat ccgcccggat gccgcggcgc cggtggccga gctccaggcg
     3301 cgcgcccgtg ccgcctacga gcaggggccg cccgcgccca ccgccgacga gacgggtcgc
     3361 cgccgcaatc tggcgattga cacgctgctg gacgcccaag atgtgctgga atccgacccg
     3421 ctggcggcca cgctggtgct gaacaatgcg ctggagcagg tgctgcgctg gtactacgcc
     3481 gaacgccggg cctggatgcc caagctcaag cgcctgcccg ccgacctggc gacccgcgac
     3541 caggccctgg ccggcgccgt gcgctcctat ctggtggcgg cggatgcccc ggcccgccac
     3601 gccgccctgc taggcgtgtt cgctttggtg tatcccgccg ggcacggcct cgatcaatgg
     3661 tgctgggaga gcacccccga accctttagc gagtaagaag gagcagcaac gatggtcacg
     3721 gcccttgtga tgatcaacgc cgagcggcac acggtgggcg agacggccca ggccgtgctc
     3781 aagatcgcgg gcgtgcgcga agtctattcc gtcaccggcg agtacgacct ggtcgccatc
     3841 gtcagcacgc cggtctacga gcaactggcc gacctggtga ctcgcgacat caccgcggtg
     3901 cgcacgatca cccgcacgca taccctgatg gccttccagt gctactccaa ccaggacatg
     3961 gagcgcctct gggacatcgg ctcggaggaa gctgagcccg aggtataggg ccaccggcgg
     4021 ctctccgcat cggccacgta tccccggcgg cgggccgcgc gatcagggcg ccagatcttg
     4081 catggcgacg gcatgttcgc cgggttcgag atagtgggca cgctgggcat agatctgggc
     4141 cacgcgccgg agatccgggc ggaaggcttc cgccagcgcc tgcttctgcg tgtagcgatc
     4201 cccctgtttg cgcatatgct ccacctgggc ctggatcatg ccccggcgcg atccgaggta
     4261 gccccaggga aagcgcccat cgagcatttc gtccaggata accgcaaaat agtgctcctg
     4321 ggcgcgctgc cgggtcgccg ggtcgagttg gtagtagctc ttcatcgtta ttccactggg
     4381 ggcaggtgcg tggttttgtg gtcgggcacg gctgcgccgt ccgccgtggg ggcgggcagc
     4441 acgccccagc cgtagcgttg cgcctcgggg ctgcccccga cccacgtgcg ggtcagccgg
     4501 tgcgtgggtg cgccggcagg caggctgagt ggggcgggga atttgtccgc cagggtaccg
     4561 gcgggcacgt tttcgggcag gcgcagcggg cgcgggatac cgccgagcca tcgccagcgc
     4621 agatcatagg cggcgatgac ctcggtggta aagcggtgga ccaacgcctg ggcctccgcc
     4681 tcgcgcccgc gcggaatggg cacgcgcagc aggacacggc gccgcccgcg cccttgcccc
     4741 cggtccagcg caaactccag gacatcgggg gcgccggcgc ggatctgcac ggcacgaggc
     4801 cgcagatccg actcgacgag cggggtgtag catccggcct cgaccagccc gcgggcgccg
     4861 atgcggattt ggcacgaccc ggcggcccgc gcctggcggc gttgcagcac ccggcccagg
     4921 cggagccggt gctcgatcc
//
LOCUS       DASUYC010001016         2537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_2020000, whole genome shotgun sequence.
ACCESSION   DASUYC010001016 DASUYC010000000
VERSION     DASUYC010001016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2020000"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1863)
                     /locus_tag="VF276_16625"
     CDS             complement(<1..1863)
                     /locus_tag="VF276_16625"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX6608528.1"
                     /translation="MAERVAVVIGINYTHFPGGVPGEAATRAGLNALHFAEADAREMA
                     ATLQASGYDVAPLLGIAATRVAIIDALRRQSRAAGDTGLLLVYFAGHGQIDPDDPDTA
                     YLLPVDADPQNPADRAIPLDELAQRLLGRVRTALTLIDCCHSGYAVGLRGGAEPATAG
                     REFNRLAQNTFRNVRGRIALTACAGNQLAREVPRLQHGAFTYYALDWWRRSAETDDLS
                     LTSYVAAGLEKEGLPAPVRGGVQEGRIVLRPLLPKEPARPAVDLAWRQEIYQLLRPLD
                     DRQLRHLSYLLGFDYDALPGEDRQARTLRLILEIEAAGRTAELTDAAREAAARQTRDA
                     TLYRRLLDDLDPARLPALAAAVGINYNTLPGPGPEDKLRRLIDEVEWTGRFADLEAAA
                     QTAFAQQTRDRAAQAEAARQAQAAAARQTVELDAREATPIPPPVAVAPPARDKARPAP
                     QAPVVKTEPVAPGMSAWNPPPRADVMTLAAPLVAPAPVAQPGPAFMIRRALGMNLLVA
                     GLYTLAYIGVAVLWEVAAQSSLLRQVAQSLLGPVPFPRESAIVAARAMSFAGDFAYWL
                     TSPQVYFWQAVYQSFPWVQGVLMVLGAILLFVARQRRNSGMRAYGSGLVIPSPVF"
     gene            complement(1976..>2537)
                     /locus_tag="VF276_16630"
     CDS             complement(1976..>2537)
                     /locus_tag="VF276_16630"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX6608529.1"
                     /translation="LDAIAAVRPLRAAWVVGHTPATAAAFVAAHGARFAFPLTAIAGP
                     DAIRAALGEAAIVATCTNAAEPLFDGAWLQPGTHINAVGSYRAATREVDTTTIARARV
                     LVDTREGALSEAGDLLIPLAAGAITLDHIRGELADLVGDRVALRESPADITLFKSVGF
                     ALEDAATAALACARAEERGIGVEVAF"
BASE COUNT          400 a          842 c          922 g          373 t
ORIGIN      
        1 aaaaacaggc gatggaatga ccagaccgga tccgtaagcc cgcatcccgc tatttcgccg
       61 ctgccgggcc acaaaaagca gaatcgcgcc gagaaccatg agcacacctt gcacccaggg
      121 aaacgactgg tagacagcct gccagaaata aacttgcgga ctagtgagcc agtaagcaaa
      181 gtcaccggcg aaggacatcg cacgcgcggc cacgatggcg gactctctgg gaaacgggac
      241 aggtcctaat agcgattgcg cgacttgccg gagaaggctg ctctgcgcgg cgacttccca
      301 caggacggcc actccgatgt atgcgagcgt gtacaatccc gcgaccagca gattcatgcc
      361 aagcgcccgc cggatcatga aggcagggcc aggctgggca acgggcgccg gggcgaccag
      421 cggcgccgcg agagtcataa catccgcgcg gggcggcggg ttccacgcgc tcattccggg
      481 cgccacaggc tcggtcttga cgaccggcgc ctgcggtgcc ggtcgggcct tatcgcgggc
      541 ggggggcgcg acggcgacgg gaggcggtat aggagtggct tccctggcat ccagttcgac
      601 cgtctggcgg gcggcggcag cctgcgcctg gcgcgcggct tcggcttgtg ccgcccggtc
      661 gcgggtctgc tgggcgaacg cggtctgcgc cgctgcttcc agatcggcga agcgcccggt
      721 ccactcgact tcatcgatca agcgccgcag tttatcttcg gggccggggc cgggcaaggt
      781 gttatagttg atcccaactg cggcagccag ggcgggcagg cgggcgggat ccaggtcgtc
      841 cagcagccgc cggtagagcg tcgcatcgcg ggtctgccgg gcggcagcct ccctggcggc
      901 gtcggtgagc tcggcggtac gcccggcggc ttcgatctcc aggatcaggc ggagcgtgcg
      961 ggcctggcga tcttcgccgg gcagggcgtc gtagtcgaaa cccagcaagt agctcagatg
     1021 gcgcagttgc cggtcgtcca gcgggcgcag cagttggtag atctcttgtc gccacgccag
     1081 atccacggcg ggccgggccg gttccttcgg cagaagcggg cgcagcacaa tccggccttc
     1141 ctgcacgccg ccgcgcaccg gcgcgggtaa gccttccttt tccagccccg ccgccacata
     1201 gctggtcagg ctaaggtcgt cagtctcggc gctgcgccgc caccaatcga gtgcgtagta
     1261 ggtgaaggcg ccgtgttgca gccggggaac ctcccgcgcc agttggttgc cggcgcacgc
     1321 ggtgagggca atacgcccgc gcacattgcg aaaggtgttc tgcgccagcc ggttgaactc
     1381 gcgccccgcg gtggccggct cggcgccgcc gcgcaggccg accgcatagc cgctgtggca
     1441 gcaatcgatc agcgtcagcg ccgtgcggac gcgccccagc aggcgctgag ccagttcgtc
     1501 cagggggatc gcccggtcgg cgggattctg ggggtcggcg tcgacgggca gcaggtaggc
     1561 ggtatcgggg tcgtcggggt cgatctggcc gtgcccggcg aagtagacga gcagcaagcc
     1621 cgtgtccccg gcggcgcggc tctggcggcg cagagcgtcg atgatcgcaa cgcgggtggc
     1681 cgcgatgccc agcagcgggg ccacgtcgta gcccgacgcc tgcaacgtgg cggccatctc
     1741 gcgcgcgtcg gcttcggcga agtgcagcgc gttcagcccg gcacgggtag ccgcctcacc
     1801 cggtacgccg ccagggaagt gggtatagtt gatgccgatg acaacggcga cgcgctcggc
     1861 caccgattct ccccccttac aacaaccggg gttcgtgttt tgtgcttcca gtatagcacc
     1921 gaatccgggc cggtacaatc gccgctttct cggcgcggcg agaaagcggc gtcttctaga
     1981 aggccacctc cacgcctatc ccgcgttcct ccgcgcgggc gcaggccagg gccgccgtgg
     2041 ccgcgtcttc caacgcgaag ccgaccgact tgaacagcgt gatgtcggcg ggcgactcgc
     2101 gtagggcgac gcgatccccc actagatcgg ccagttcgcc gcggatgtga tcgagcgtga
     2161 tcgcgcctgc ggccagcggg atgagcaggt cgcccgcctc ggatagcgca ccctcgcggg
     2221 tatcgaccag cacacgggcg cgggcgattg tggtcgtgtc cacttcgcgg gtcgcggccc
     2281 ggtagctgcc gacggcgttg atgtgcgtgc cgggttgcag ccaggcgccg tcgaacagcg
     2341 gttccgcggc gttggtgcag gtggcgacga tggcggcctc acccagggcg gcgcggatcg
     2401 cgtccggccc ggcgatagca gtcagcggga aggcgaaccg cgcgccgtgc gcggcgacga
     2461 aggccgcggc ggtcgccggc gtgtggccca ccacccaggc ggcccgcagc gggcgcacgg
     2521 cggcaatggc gtcgaga
//
LOCUS       DASUYC010001017         5679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1325320, whole genome shotgun sequence.
ACCESSION   DASUYC010001017 DASUYC010000000
VERSION     DASUYC010001017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5679
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325320"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1251
                     /locus_tag="VF276_16635"
     CDS             <1..1251
                     /locus_tag="VF276_16635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6608530.1"
                     /translation="MAEFDWLRGLSFIYAGPRGIYPHDLAREALEADLKWRDPEAFGA
                     FHRRAHEYYVLRMRSPQDDDDNYHDFVYLHKNPRIRAVFAWDQMHGLRATAPWPTDWA
                     HLRSMVARHEGAEAAALADFWYTRQPEGVTVIRRDDGPPEGFLFHLILRPADAATYAA
                     EPATAAAWRYIEATTPLESDEVASLLRFWMAADSYQAVSAVQGVAFVIAARHVITTPH
                     LVRTSFVFADPPAWEAVMAHFDLTRLPAGDFTVGGHTYGVFTHDWRARSPTAWLAAMA
                     DREVTEELAAPLDAPPGPEVVLDQAAFAAAVRDALRDLGDRTALAANPLLGTPLVARR
                     VPPTAGETERAAALRAVVLEALAPLAANPRETPFYQALYYTYVVPVGSQDRAARRLGV
                     PFSTFRRHLKAGTDRLIAALWALR"
     gene            complement(1540..1986)
                     /locus_tag="VF276_16640"
     CDS             complement(1540..1986)
                     /locus_tag="VF276_16640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608531.1"
                     /translation="MSGHNPPDRVAEILRAQRAAMIDVIARRIHQEIPDYHNVPLAVV
                     TERFTAIVDAMIASLIAYDPTHLTQILEHTGRQRIVQGYGIGALLTTADIVEQTMGPV
                     LTRELADDPELQANTMRRLTSLVRTARHILSRINLDEVVKQGPKKG"
     gene            2182..3651
                     /locus_tag="VF276_16645"
     CDS             2182..3651
                     /locus_tag="VF276_16645"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6608532.1"
                     /translation="MAVSPARPALVTPPDPSAAVTRACIGATILTAAPTPAADARGGP
                     QAPPPENHMATPAFPTVMPHRLPIFALLIANAVSLVGSTLTSIAVPWFVLLTTGSAAK
                     TGLTAFFSLLPLVLGGFFGGALVDRLGFKRTSVGADLLSGVAVAMIPLLYAAGHLPFW
                     ALLILVFVRNLFDIPGGTARQSMIPDLARAAALDLGRANASVESIRRFATLLGPPLAG
                     VLIAAAGQSNVLYLDAASFLVSATVVGVAVPALRRERSGPAHYLTDLREGLRFLHADR
                     LVVALITVLALTNVLLNPLFIVILPVYANVTSGSALNLGLMIGAFGGGTLAGAILFGL
                     LGPRLPRRALLTGGMLVTGLPLWLLSTLPPLPITAAALAVMGLALGPLGPLALTVLGE
                     RTPDALRGRVFGAFGTLANMWIPLGVLATGYLLDAFGAGAIILGLAVVYLVFSLSTLF
                     IPVFHQLGTPASRDDASGPPAPAPKGASTEASPALLPEG"
     gene            3868..4206
                     /locus_tag="VF276_16650"
     CDS             3868..4206
                     /locus_tag="VF276_16650"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009632753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX6608533.1"
                     /translation="MDDLSSMKCVACRKGEPTVSEAEMAALRPQIPDWQVIEREGIPR
                     LERVFTFPNFAAALAFTDRVGALAEAEGHHPALLTEWGRVTVTWWTHKIGGLHRNDFI
                     MAAKTDQLAG"
     gene            4436..>5679
                     /locus_tag="VF276_16655"
     CDS             4436..>5679
                     /locus_tag="VF276_16655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608534.1"
                     /translation="MPTDLSCLDIPLRRNGSMFAGAGPFPDPPGAPVEWRRDAGRFPA
                     PLTPMYYSTYTALEGEGRRRVAALYADPALDRPLRRINTYAYVGSVPAASPETQAARM
                     AGSPARITAVLDRLDDLWTHRWRPELAHHLAVWDGFDLARAGLPALMAYLDESLRRGA
                     RLWELYFLLDAPARLALAAFARAYAALGGPPDPLTAQRLSAGGDDKMAETDRALADLG
                     REARATPPVADILLAGGPDVCARLDALPAGRTFLGSLGAFLYVYGRRSPGWDWGYPAW
                     LEDPAPVLAALRADLAAPARDCAAEQVAARERALAEARERARRAPAAVAAEFACALPL
                     ACAARRIREDARFYVVLSGMYRVRRVILECGRLLQAAGLLGAAGDVFALTLDELRATA
                     QARPPADLRVLAALRQAEMAHWA"
BASE COUNT          714 a         2081 c         1991 g          893 t
ORIGIN      
        1 atggccgagt tcgattggct gcgcggcctc tctttcatct atgccggacc ccgcggcatc
       61 tacccccacg acctggcccg cgaggcgctc gaagccgacc tgaaatggcg cgatccggag
      121 gcgttcggcg cgttccaccg gcgggcgcac gagtactacg tgctgcgcat gcgctcgccg
      181 caggacgacg acgacaacta ccacgatttc gtctacctgc acaagaaccc gcgcatccgc
      241 gccgtgttcg cctgggacca gatgcacggg ctgcgggcga ccgcgccgtg gcccaccgac
      301 tgggcccatc tgcgatccat ggtggcccgg cacgaaggcg cggaagcggc ggcgctggct
      361 gacttctggt acacgcgcca gccggagggc gtcacggtca tccggcgcga cgacggcccg
      421 cccgaaggct tcctgttcca cctcattttg cgccccgccg acgcggccac ctacgccgcc
      481 gagccggcga ccgccgccgc ctggcgctat atcgaggcga ccacgcccct ggagagcgac
      541 gaggtggcga gcctgctgcg cttctggatg gccgccgaca gctaccaggc cgtctcggcg
      601 gtgcagggcg tggccttcgt catcgcggcc cgccacgtca tcaccacgcc gcacctggtg
      661 cgcaccagct tcgtcttcgc cgacccgccc gcctgggaag cggtcatggc ccacttcgac
      721 ctgacccgcc tgcccgccgg cgatttcacc gtgggcggcc acacctacgg cgtcttcacg
      781 cacgattggc gcgcccgctc gcctaccgcc tggctggcgg ccatggccga ccgggaggtg
      841 acggaggaac tggccgcgcc gctcgatgcg ccgcccggtc cggaggtggt gctcgatcag
      901 gccgcgtttg ccgccgccgt gcgcgatgcc ctgcgcgacc tgggcgaccg cacggccctg
      961 gccgccaacc cgctgctggg cacgccgctg gtcgcccggc gtgtgccgcc caccgccggg
     1021 gagaccgagc gcgccgcggc gttgcgcgcc gtggtgctgg aggcgctggc gccgctggcc
     1081 gcgaatccgc gcgagacgcc gttctaccag gcgctctact acacctacgt ggtgccggtg
     1141 ggcagccagg accgtgcggc gcggcgcctg ggcgtgccct tcagcacctt ccgccgccac
     1201 ctcaaggccg gcaccgaccg gctcatcgcg gcgctgtggg cgctgcggta gccgggtagc
     1261 ccggaggctc aagcctccgg ctaccattac gaagcccgcc tgcgcgggct gcttgggatt
     1321 gacccagccc gcgcaggcgg gctttgtaga ggtagcccgc tccttgaggg gcggggcggc
     1381 gccgccgaag tgtgccgcta acgggccgcg ccgtctatct cccccttccc gcgccgggaa
     1441 ggtgagtggc tgcaaacgta ctcgatgccc gacgacagcg ccgcgccgtc tatctccccc
     1501 ttcccgcgcc gggaaggggg ccggggggtt aggcccgtct cagcccttct tcggcccctg
     1561 tttcacgacc tcgtcgaggt tgatgcggct gaggatgtgg cgcgcggtgc gcaccaggct
     1621 ggtcaggcgg cgcatcgtgt tcgcttgcaa ctccgggtca tcggcgagtt cgcgggtcag
     1681 caccgggccc atggtctgtt ccacgatgtc ggcggtggtc agcagggcgc cgatgccgta
     1741 gccctgcacg atgcgctggc gccccgtgtg ctccaagatc tgggtcaggt gtgtcgggtc
     1801 gtacgcgatg aggctggcga tcatggcatc gacaatcgcc gtgaagcgct cggtgacgac
     1861 ggccagtggg acgttgtgat aatcggggat ttcctggtgg atacgccggg cgatcacgtc
     1921 gatcatggcg gcgcgctggg cgcgcaagat ctcggcgacc cgatccgggg ggttgtggcc
     1981 gctcatagca ggttggttgc tccatgtaag taaggtcggg cgccgcaagc gcaagccggc
     2041 gccagatgcg gtcgcggtcc ctgcattggg ctggaggatg gtcgcgccga tccgcgcccc
     2101 cattatagca catccggtcc ccggcggcag cgcccggttg gccgcggtgg cgcatttaca
     2161 aggcgcgggc gacgtgctat catggcagta agcccggcgc ggcccgccct ggtcaccccg
     2221 ccggacccct ctgccgccgt cacccgcgcc tgtatcggcg cgacgatctt gaccgctgcg
     2281 cccacgcccg cagccgatgc tcgcggcggg ccccaggccc cgcccccgga gaaccatatg
     2341 gcgactcccg ccttccccac cgtcatgcct caccggctgc ctatcttcgc cctgctgatc
     2401 gcgaacgcgg tgtccctggt cggcagcacg ctgaccagca tcgccgtgcc ctggttcgtg
     2461 ctgctgacca ccggcagcgc cgccaagacc ggactgaccg cgtttttctc gttgttgccg
     2521 ctggtgctgg gcggcttctt cggcggcgca ctggtcgacc gcctgggctt caagcgcacc
     2581 agcgtcggcg ccgacctgct gagcggcgtg gctgtggcca tgatcccgct gctctacgcg
     2641 gccggccacc tgcccttctg ggcgttgctg atcctggttt tcgtgcgcaa cctgttcgac
     2701 atccccggcg gcaccgcccg ccagagcatg atccccgacc tggcgcgcgc cgccgcgctg
     2761 gacctggggc gggccaacgc ctcggtcgag tcgatccggc gcttcgcgac gttgctgggg
     2821 ccgccgctgg ccggggtgct gatcgccgcc gccggccaga gcaacgtgct gtacctcgac
     2881 gccgcgtcgt tcctggtctc cgccaccgtg gtcggggtgg ccgtgcccgc gctgcggcgc
     2941 gagcgcagcg gcccggcgca ctacctgacc gacctgcgcg agggcctgcg cttcctgcac
     3001 gccgatcggc tggtggtcgc gctgatcacc gtcctggcgc tgaccaacgt gctgctcaac
     3061 ccgctgttca tcgtcatcct gccggtttac gctaacgtca cctccggcag cgcactgaac
     3121 ctggggctga tgatcggggc attcggcggg ggcacgctgg cgggtgcgat cctgttcggc
     3181 ctgctggggc cgcggctgcc gcgccgcgcc ctgctcaccg gcggcatgct cgtcaccggg
     3241 ctgccgctct ggctgctcag caccctgccg ccgctgccga tcaccgccgc cgcgctggcc
     3301 gtgatgggcc tggcgctggg accgctgggg ccgctggcgc tgaccgtgct gggtgagcgc
     3361 acccccgacg ccctgcgcgg gcgcgtcttc ggcgccttcg gtacgctggc gaacatgtgg
     3421 atcccgctag gcgtgctggc gaccggctat ctgctcgacg ccttcggcgc gggggcgatc
     3481 atcctgggcc tggccgtggt gtacctggtc ttcagcctca gcacgctgtt catcccggtc
     3541 ttccaccagt tgggcacgcc cgcgagccgg gacgacgcct ccggcccacc tgcgcctgcc
     3601 cctaaagggg catctacaga agcatcgccc gcactgctgc ccgaaggctg agtagttttt
     3661 ccaaatgtct ttgtcactct gagcgcagcg aagagtctcg tcttgtgccc caacgagacg
     3721 agattctgag cgcagcgaag aatgacaatg cttgggtgac agactaccag ggcgccggcg
     3781 tcctgctcgc gtggtatact ggggttgcaa cgacacggta tacaggcggg cggcggcccc
     3841 aggcggcgcc cgcagggggg caagacgatg gacgatctga gcagcatgaa atgcgtggcc
     3901 tgccgcaagg gcgagccgac cgtcagcgag gccgagatgg cggcgctgcg cccgcaaatc
     3961 cccgactggc aggtgatcga gcgcgagggc ataccgcgcc tggagcgggt gttcacgttc
     4021 cccaacttcg cggcggcgct ggccttcacc gaccgggtgg gcgcgctggc cgaggccgag
     4081 ggtcaccacc cggcgctgct gactgagtgg ggccgcgtca ccgtcacctg gtggacgcac
     4141 aaaatcggcg gcctgcaccg caacgacttc atcatggccg ccaaaaccga ccaactggcc
     4201 gggtaagccg cctgcaacac cgccctccgc ttgcggtgta ggggccggtt tcaaaccggc
     4261 cggctgccca atgcacggcc ggcgccccac cgaggtgggc attcgtctcg atgggaaggg
     4321 gacaggcgct ccggaatctg cggaaacttc cgcgcggcat acggcaccct tcgccgcgcg
     4381 gatcgggttt actggagaca ggcccgtaac ccacccgcag aaggaggcga cccctgtgcc
     4441 aaccgacctg tcctgtctcg atattcccct ccggcgcaac ggcagcatgt ttgccggggc
     4501 cggcccgttc cctgacccgc ccggcgcgcc ggtcgagtgg cggcgggatg ccggccgctt
     4561 tcccgcgccg ctgacgccga tgtactactc cacctacacc gcgctggagg gcgagggccg
     4621 gcggcgcgtg gccgccctgt acgccgaccc cgcgctcgac cgcccgctgc ggcgcatcaa
     4681 tacgtatgcc tacgtgggta gcgtgcccgc cgcgtcgccg gagacgcagg cggcgcgcat
     4741 ggcgggcagc ccggcgcgca tcacggctgt gcttgaccgg ctggacgacc tctggacgca
     4801 ccgctggcgg cctgaactgg cgcatcacct ggccgtctgg gatggattcg acctggcgcg
     4861 cgccggactg cccgcgctga tggcctacct ggacgagagc ctgcgccggg gcgcgcgcct
     4921 gtgggagcta tacttcctgc tcgatgcgcc cgcccggctg gcgctcgccg cgttcgcgcg
     4981 ggcctatgcc gcgctgggcg ggccgccgga tccgctcacc gcgcaacggc tgtcagcggg
     5041 cggcgacgat aaaatggcgg agacggaccg ggccctggcc gatctgggcc gggaggcgcg
     5101 cgccacgccg ccggtggcgg acatcctgct ggccggtggg ccggatgtct gcgcgcggct
     5161 cgacgccctg cccgccgggc gtacctttct ggggtcgctc ggcgcgttcc tctacgtcta
     5221 cgggcggcgc agccccggct gggactgggg ctaccccgcc tggctggagg acccggcgcc
     5281 cgtgctggcc gcgctgcgcg ccgatctggc ggcgcccgcc cgcgattgtg ccgccgagca
     5341 ggtcgccgcc cgcgagcgcg cgctggccga ggcgcgcgag cgggcacggc gcgcgccggc
     5401 tgccgtggcg gcggagttcg cgtgcgcctt gccgttggcc tgcgccgcgc gccgcatccg
     5461 cgaggacgcc cgcttctatg tcgtcctgag cggcatgtac cgcgtgcgcc gggtgatcct
     5521 ggaatgcggg cggttgctcc aggcggcggg cctgctcggc gccgcaggcg atgtcttcgc
     5581 cctgacactg gacgaactcc gcgccacggc ccaggcgcgc ccgcccgccg acctgcgcgt
     5641 gctggccgcg ctccgccagg ccgagatggc gcactgggc
//
LOCUS       DASUYC010001018         2764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_633532,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001018 DASUYC010000000
VERSION     DASUYC010001018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633532"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="VF276_16660"
     CDS             complement(<1..454)
                     /locus_tag="VF276_16660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608535.1"
                     /translation="MPRHAALLLIGLLAGLVVLLAAPVPIHAAPRRAPLPQAATLDRA
                     AYVALLHTALDAANAVPAAADAPDRAALLARARAVLPESVVVRDAGGVLAEVDFGPVR
                     AALAKDPPDLAGARRYLTALIGLLDPGSLPSPTATPTPLQTPSAAQAAL"
     gene            complement(457..1452)
                     /locus_tag="VF276_16665"
     CDS             complement(457..1452)
                     /locus_tag="VF276_16665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608536.1"
                     /translation="MSSTPTPAESVPMPAPGAVTPATAAAAPARPRSAWTLAGVAWRL
                     YMANRALFVLPVLVLELPLLLLTVGVLALLDPGPTALTVDWVTNFTIFTLAHSTLTLV
                     EAVLAFLLLAIIAVQIDTRLRGGRLSLSAAFRRVLPRLGALLGGSFLLVVTVGLLTVL
                     GVVLSVVFSLVLTVINADPASGGLAGAIQRGLADPTQNIWLRLLLLAVVAGAAIFLVV
                     KWSLMVQVVILEDAPPVHSLRRSWQLVTGQFWRTLGVLLIGMLPLTLLSNGTLVVEFF
                     GLLPAGPDRVLILALARGLGLFLRLLLLPWALALLTLYYYDLRLRRESAAPRAAP"
     gene            complement(1449..2465)
                     /locus_tag="VF276_16670"
     CDS             complement(1449..2465)
                     /locus_tag="VF276_16670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608537.1"
                     /translation="MAAHATSTAETPAAGRHQPLTRLRPRDIGSLLDQAVILYRHNFM
                     TWVGIVALIEVPVSIVLTLATVTLLDPSTLARTPRLPGPDAGPDAYAGYQAATVAALS
                     DLLSRAFLVTAITVVGAILLNIASGALARVIAENYMGGSMGVAAAYRAIRPRIPALIG
                     VMLIVMLSTVLVLIPPLFLWIYISLSFVSQVIVLENRGVGDALQRSWELVRGSWWRVF
                     GAYLLLLLVGMIVNLSTTVVSLAVGLTGASWAVQNIAVQFVTLLLTVVYRPISLAGMT
                     LLYFDLRIRKEGYDLQVALDERARETGLAPAPAGAAWPYREAAPAAPAATPAGWPYGE
                     ATQA"
     gene            complement(2532..>2764)
                     /locus_tag="VF276_16675"
     CDS             complement(2532..>2764)
                     /locus_tag="VF276_16675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX6608538.1"
                     /translation="ALMEKHHVTAVYAGHLHAYTRVERNGIPYIITGGGGEPLHVPAL
                     LGGRYHYLRVAVRGTTASDTVVLLNNVPTPGP"
BASE COUNT          470 a          952 c         1024 g          318 t
ORIGIN      
        1 ccagggcggc ctgcgcggcg ctgggggtct ggagcggcgt gggggtcgcc gtcggcgagg
       61 gcaggctgcc ggggtccagc aggccgatca acgcggtcag gtagcggcgc gctccggcca
      121 ggtcgggcgg gtctttcgcc agcgcagcgc gcaccggccc gaaatcgacc tcggccagca
      181 ccccgcccgc atcacgcaca accacgcttt ccggcagcac ggcgcgggca cgcgccagca
      241 aggcggcccg gtcgggcgca tcggcggcgg cgggcacggc gttggccgcg tcgagcgcag
      301 tgtgcagcag ggccacgtag gcggcgcggt cgagcgtcgc ggcttgcggc agcggggcgc
      361 ggcgtggcgc ggcgtggatc gggaccggcg ctgccagcag caccaccagc ccggccagca
      421 aaccgatcag caacagagcc gcgtggcggg gcatcgtcag ggtgcggccc tcggcgccgc
      481 gctttcccgg cgcaggcgca gatcatagta gtagagggtc agcagggcca gcgcccacgg
      541 cagcagcaac aggcgcagga acagcccgag accgcgcgcc agcgccagga tcagcacgcg
      601 gtccggcccg gccggcagca gcccgaagaa ctcgaccacc agcgtgccgt tggagagcag
      661 cgtcagcggc agcatgccga tcagcaacac gcccagcgtg cgccagaact gccccgtgac
      721 caactgccag ctgcggcgca gcgagtggac cggcggcgcg tcttcgagga tgaccacctg
      781 caccatgagc gaccacttga cgacgaggaa gatcgccgcg cccgcgacga cggccagcag
      841 caacagccgc agccagatat tctgggtggg gtcggccagg ccgcgctgga tagcgccggc
      901 caacccaccg ctggcggggt cggcgttgat caccgtcagc acgaggctga acacgaccga
      961 cagcaccacg cccagcacgg tcagcaggcc gacggtgacg accagcaaga acgagccacc
     1021 cagcagggcg cccagccgcg gcagcacgcg ccggaaggcc gcgctcaggc tcagccgccc
     1081 gccgcgcaag cgggtatcga tctgcacggc gatgatcgcc agcagcagga aggccagcac
     1141 ggcttccacc agcgtcagcg tgctgtgggc cagggtgaag atggtaaagt tggtgaccca
     1201 atcgaccgtc agggcggtgg ggccggggtc gagcagggcc aggacgccga cggtgagcag
     1261 cagcagcggc aactccagca ccagcaccgg cagcacgaac agcgcgcggt tcgccatgta
     1321 gagccgccag gccaccccgg ccagcgtcca cgcgctgcgc gggcgggcgg gcgctgccgc
     1381 cgccgttgca ggcgtcacgg cgcccggcgc gggcatgggg accgactcgg cgggcgtggg
     1441 ggtgctgctc atgcctgggt cgcctcgccg tagggccagc cggcgggtgt ggccgccggg
     1501 gcggcggggg cggcttcgcg gtagggccag gcggcgcccg ccggggccgg ggccagcccc
     1561 gtctcgcgcg cgcgctcgtc cagtgccacc tgcaagtcgt agccttcctt gcggatgcgc
     1621 aggtcgaaat agagcagcgt catcccggcc aggctgatcg ggcgatagac cacggtgagc
     1681 agcagggtca cgaactgcac ggcgatgttc tgcacggccc acgaggcgcc cgtcaacccc
     1741 acggccagcg acaccaccgt ggtcgagagg ttcacgatca tgccgaccag cagcaacagc
     1801 agataggcgc caaacacgcg ccaccacgac ccgcgcacca gttcccaact ccgttgcagc
     1861 gcgtcgccca cgccgcggtt ctccagcacg atcacctggc tgacaaagct caggctgata
     1921 tagatccaga ggaacagcgg cgggatcagc accagcacgg tcgagagcat gacgatcagc
     1981 ataacgccga tcagggcggg gatgcggggc cggatggcgc ggtaggccgc cgccacgccc
     2041 atcgacccgc ccatgtagtt ctcggcgatg acccgcgcca gcgcgccgga ggcgatattc
     2101 agcagaatgg ccccgacgac ggtgatggcg gtgacgagga aggcgcggct gagcaggtcg
     2161 ctgagcgccg ccacggtggc cgcctggtag ccggcgtagg cgtccggccc ggcgtccggt
     2221 cccggcaggc gcggcgtgcg cgccagggtg ctcggatcga gcagcgtcac ggtcgccagg
     2281 gtgaggacga tgctcaccgg cacctcgatc aaggcgacga tgcccaccca ggtcatgaag
     2341 ttgtgccggt agaggatcac cgcctggtcg aggaggctgc cgatgtcgcg gggccgtagc
     2401 cgggtcaacg gctggtgccg gcccgcggcg ggcgtctcgg cggttgaagt tgcatgcgca
     2461 gccatgatct gactccctgt cgctgcatct taccacaagc ctatgacgcg gcgcaatggc
     2521 cggagcgtcc atcaggggcc gggcgtcggc acgttgttca acaagaccac cgtgtcgctg
     2581 gcggtggtgc cgcgcacggc cacgcggagg taatggtagc gcccgcccag cagcgcgggc
     2641 acatgcagcg gctcgccgcc gcccccggtg atgatgtagg ggatgccgtt gcgctcgacg
     2701 cgggtatagg cgtgcagatg gcccgcgtag acggcggtga cgtggtgttt ctccatcagc
     2761 gcca
//
LOCUS       DASUYC010001019         2057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_2020174, whole genome shotgun sequence.
ACCESSION   DASUYC010001019 DASUYC010000000
VERSION     DASUYC010001019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2020174"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VF276_16680"
     CDS             complement(<1..230)
                     /locus_tag="VF276_16680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608539.1"
                     /translation="MSGRPPSPTGAGPSRRYLYVAYTLSLTLKAANAVQTYQTARHLR
                     DLAATQGAALTLVMPRWLRERSLFDAVLGPAVR"
     gene            complement(227..1354)
                     /locus_tag="VF276_16685"
     CDS             complement(227..1354)
                     /locus_tag="VF276_16685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6608540.1"
                     /translation="MKVALYNVTTTTKVGGVETFVWEVAARLAQRGYRVDVIGGRGPE
                     RRPAPGVRVVTLPYIDRALWRRIPLLGRQYTLTKLLERLSLAVAGLPYLLVHRYDIVH
                     IQKPYDLPVAAVCRLVGTRAIFGCHGKDFWLGDRLFTRAMNAAVSCSAYNAGQIRARY
                     GISPTVIYNGVDTTQFQPTPPPDMAATLAALGLEPRPPTILFAGRLVRWKGVEYLIEA
                     LAQLRTPGAQVWIAGSGPYQPDLEALAARLGVADQVRFLGTLPTDQLTALYHAADLLA
                     ATSFVNETFGIAPAEAMACARPVVASRFGGFTEVVEDGVTGYLVRPQDPADLARCIDA
                     LLADPAHATAMGEAGLARVRRLFTWEAVVDRLERVYRRVAA"
     gene            complement(1351..>2057)
                     /locus_tag="VF276_16690"
     CDS             complement(1351..>2057)
                     /locus_tag="VF276_16690"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6608541.1"
                     /translation="LGGQFAGKRVLDLGSGMGGGLVALRRAGLEVVGLDYNPAYCRIA
                     RLRARRHGVHLPLLVGAGEQLPFPDKGFDVVINLDVLEHVADPERVVSEIARVLKPGG
                     ACLTSAPNRFAFRDAHYHLPGINWLPRPWAEAIIRRSGHSKEEDSLLTDRQALSALNT
                     YTWAGFAALARRHGLRAVDAVEERLRRGEIRQLTGVRRTLLDVVRRLGLLVPIYRAYR
                     FGWQGTFQARLEKPRR"
BASE COUNT          303 a          746 c          751 g          257 t
ORIGIN      
        1 cgcaccgccg ggcccagcac ggcgtcgaac agactccgct cgcgcaacca gcgcggcatc
       61 accagcgtca gcgcggcgcc ctgggtggcg gccaggtcgc gcaggtggcg cgccgtctga
      121 tacgtctgca cggcgttggc cgccttgagc gtcaggctca gcgtgtaggc gacatagaga
      181 tagcggcggg acgggccagc gccggtaggg gacgggggcc tgccgctcat gccgccaccc
      241 gccggtagac ccgttcgagc cggtccacga ccgcctccca ggtgaagagc cgccgcaccc
      301 gcgccagccc ggcctcgccc atggccgtgg cgtgcgccgg gtcggccagc agagcgtcga
      361 tgcaccgggc caggtcagcg gggtcttgcg ggcgcaccag gtagccggtc acgccgtcct
      421 cgaccacctc ggtgaagccg ccgaagcggc tggcgacgac cgggcgggcg caggccatgg
      481 cctcggcggg cgcgatgccg aaggtctcgt tgacgaagga ggtcgcggcg agcaggtcgg
      541 cggcgtggta gagggccgtg agttggtccg tgggtagggt gcccaggaag cgcacctggt
      601 ccgccacgcc cagccgcgcc gccagcgcct ccaggtccgg ctggtagggg ccgctgcccg
      661 cgatccagac ctgggcgccc ggcgtccgca gttgggccag cgcctcgatc agatactcga
      721 cgcccttcca gcgcaccagc cgcccggcaa acaggatcgt gggcgggcgc ggctccagcc
      781 ccagcgcggc cagggtggcg gccatatcgg gcggcggagt cggctggaac tgcgtggtgt
      841 ccacgccgtt atagatgacc gtcgggctga tgccgtagcg ggcgcggatc tgcccggcat
      901 tatacgcgct gcacgacacg gcggcgttca tcgcccgcgt gaacaggcgg tcgcccagcc
      961 agaagtcctt gccgtggcag ccaaagatgg cccgcgtgcc cacgagacgg cagacggcgg
     1021 ccaccggcag gtcgtagggc ttctggatat gcacaatgtc gtagcggtgg accagcaggt
     1081 agggcaggcc cgcgacggct agcgagagcc gctccagcag cttggtcagc gtgtactggc
     1141 ggcccagcag cgggatgcgc cgccacagcg cccggtcgat atagggcagc gtgaccacgc
     1201 gtacgccggg ggccgggcgc cgctccggcc cccgcccgcc gatcacgtcc acccggtagc
     1261 cgcgctgcgc cagccgcgcc gccacctccc agacaaacgt ctcgacgccg cccaccttgg
     1321 tcgtggtggt cacgttgtag agcgccacct tcatcggcgg ggcttttcca ggcgggcctg
     1381 gaaggtgccc tgccacccga agcgataggc ccgataaatc ggcaccagca ggcccaggcg
     1441 gcgcaccaca tcgagcagcg tgcggcgcac gcccgtaagc tggcggatct cgccgcggcg
     1501 cagccgctct tccaccgcgt ccacggcgcg caggccgtgg cgccgggcca gggcggcaaa
     1561 cccggcccag gtgtaggtgt tgagggccga cagcgcctgg cggtcggtca gcaggctgtc
     1621 ttcctccttc gagtggccgc tgcgccggat gatcgcctcg gcccaggggc gcggcagcca
     1681 gttgatgccc ggcaggtggt agtgggcgtc gcggaaggcg aagcggttgg gcgcgctggt
     1741 caggcaggcg ccgcccggct tcagcacgcg ggcgatctcg ctcaccaccc gctccgggtc
     1801 ggccacatgc tccagcacgt cgaggttgat caccacgtca aaccccttgt cggggaaggg
     1861 caactgctcg cccgcgccga ccaacagcgg caggtgcacg ccatggcgcc gcgcccgcag
     1921 ccgggcgatg cggcagtagg ccgggttgta gtccaggccg accacctcca acccggcgcg
     1981 ccgcagggcc accagcccgc cgcccatacc gctgcccagg tcgagcacgc gcttgccggc
     2041 gaactggccg cccagcg
//
LOCUS       DASUYC010001020         3079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_171955,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001020 DASUYC010000000
VERSION     DASUYC010001020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171955"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            316..873
                     /locus_tag="VF276_16695"
     CDS             316..873
                     /locus_tag="VF276_16695"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX6608542.1"
                     /translation="MSVDNMDTNNQNTGSDVNGGGHDVTSTGTEADALARAEAKAAEY
                     LDLLQRERASFINFRRRTEQERAEAQQYGAAQLLKKLLPVVDDFDRALQAAPEGNAWV
                     EGVRMIDRKLHTILEQEGVTPIHSIGQPFDPTIHEAIAYEEGGEGEDVVAEEFARGYR
                     HKDKVLRHAVVKVGKGQVGEKPGNQ"
     gene            874..2007
                     /gene="dnaJ"
                     /locus_tag="VF276_16700"
     CDS             874..2007
                     /gene="dnaJ"
                     /locus_tag="VF276_16700"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX6608543.1"
                     /translation="MSATKRDYYEVLGVARGSSEDDIRKAYRKLARQYHPDINKEPDA
                     ETQFKEINEAYEVLSDTSKRSAYDRFGHAAVSGAGGGPGAGYSPFGASPFADIFETFV
                     TNAARSARAAPSRGSHLAYRMSLEFEEAIFGTDKDLEITHLENCDLCGGTGSAPGSQP
                     ITCPTCNGSGEVRRVQQSIFGQFVSLAPCERCGGDGQIISVPCERCKGDGRVRANKHL
                     VVRIPAGVDEHSQIRLAGEGDAGPNGTPPGDLYIELSIKPHKFFRRQGNDIWMDLPIN
                     IAQAALGADLQVPTVGNGTANLKVPPGTQYDKVFRLRELGVPHLRGAGRGDQLVRVKI
                     QVPATLSDHQRELLTELSQTFGEEPPQPEDKGFFGRIKDKLSS"
     gene            2153..2497
                     /locus_tag="VF276_16705"
     CDS             2153..2497
                     /locus_tag="VF276_16705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX6608544.1"
                     /translation="MTGILVRWAITIIAVFVAAKLLPTMINYDDWLGLAIFAAVLGLV
                     NAIVKPIVAILSLPVVLLTLGLFLLVINALMFWLAASLVPGFYVNGFWGALVGSIIVS
                     LVSWVLSALLPN"
     gene            2648..>3079
                     /locus_tag="VF276_16710"
     CDS             2648..>3079
                     /locus_tag="VF276_16710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011959025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HEX6608545.1"
                     /translation="MPRLPLLARGLLLVVLQPAAVALAAGPAATTVPAPDPAFAHVWA
                     RTDELVRAHDVTRSWYWGEAPFARLLEPFAGVASGTRLVEYYDKGRMEINNPAGDRRS
                     PWFVTSGLLVKELISGEMAVGLTQTEAHAPSTQPIVGDWTPE"
BASE COUNT          547 a          965 c         1032 g          535 t
ORIGIN      
        1 gcgtcgtggt ggcccgctat ggcttgccgg acaccgtcaa cggcgtgatc ggcgtcatcg
       61 gtcccacccg catgccttat ggccgggcgg tgtcgagtgt gcgctatctt tcggatctct
      121 tgacgggcat gttgcaccag atccacggcg acagtccgcg ggccgacgcg taatgcgaat
      181 ctcggggttt ctcgttttct cttgtgagat ttgcgcgtcc ctgcccggag cgaaacctgt
      241 tgccgtacta cactatagaa tatattgatg cgccggttcg gtcgggcctg ggccaaccgg
      301 tggaggaggc ttggaatgtc ggttgataat atggatacga ataaccagaa tacgggaagc
      361 gatgtcaacg gcgggggcca cgatgtgacc tcgaccggga ccgaggccga tgcgctggcc
      421 cgcgcggagg ccaaagccgc cgagtatctg gacctgttac agcgtgagcg ggcgagtttt
      481 attaattttc gccggcgcac ggagcaagaa cgggcggagg cccagcagta tggcgccgcg
      541 cagttgctga agaagctgtt gccggtggtc gacgattttg accgtgccct ccaggccgcg
      601 ccggaaggca atgcctgggt cgagggcgtg cgcatgatcg accgcaagct gcacacgatc
      661 ctggagcagg aaggcgtgac gccgatccac tcgatcgggc agcctttcga tcccacaatc
      721 cacgaggcca tcgcctacga agagggcggc gagggcgaag atgtggtggc ggaagagttc
      781 gcgcgtggct accgccacaa ggacaaggtc ttgcgccacg cggttgtcaa agtcggcaaa
      841 ggtcaggtgg gcgagaagcc aggaaaccaa taaatgagtg caaccaagcg cgactactac
      901 gaggtgctgg gcgtggcccg cgggtcgagc gaagacgata ttcgcaaggc ctaccgcaag
      961 ctggcccgcc agtaccaccc ggatatcaac aaagagccgg acgcggaaac ccagttcaag
     1021 gaaattaacg aagcctacga ggtgctgagc gatacgagca agcgctcggc ctacgaccgc
     1081 ttcgggcacg cggcggtatc cggggcgggc ggtgggccgg gggcgggcta cagcccgttc
     1141 ggtgcttcgc ccttcgccga catcttcgag acctttgtca ccaacgcggc gcgcagcgcg
     1201 cgggcggccc ccagccgggg gtcgcacctg gcctatcgca tgtcgctgga gttcgaggag
     1261 gccatcttcg gcaccgacaa ggacctggag atcactcacc tggagaactg cgacctctgc
     1321 ggcggcacag gcagcgcgcc cggcagccag ccgatcacct gccccacctg taacggcagc
     1381 ggcgaggtgc gccgggtgca gcaatcgatc ttcggccagt ttgtcagcct ggcaccgtgc
     1441 gagcgctgcg gcggcgacgg ccagatcatc agcgtgccct gcgagcgctg caagggcgat
     1501 gggcgcgtgc gggccaacaa gcacctggtg gtgcgcatcc ccgccggggt ggacgagcat
     1561 tcgcagatcc gcctggccgg cgagggcgat gccggcccca acggcactcc gcccggcgac
     1621 ctgtacatcg aactgtcgat caagccgcac aagttcttcc gccgccaggg caacgacatc
     1681 tggatggacc tgccgatcaa catcgcgcag gcggcgttgg gcgccgattt gcaggtgccg
     1741 acggtcggca acggcacggc caacctcaag gtcccgcccg gcacgcagta cgacaaggtc
     1801 ttccgcctgc gcgagctggg cgtgccccac ctgcgcggcg cgggccgcgg cgaccagttg
     1861 gtgcgcgtga agatccaggt gccggccacg ctgagcgacc accagcgcga actgctgacc
     1921 gagctgtcgc agaccttcgg cgaagagccg ccgcagccgg aggacaaggg gttttttggc
     1981 cggatcaagg ataagctgag cagctagtca cgcttcggaa tggggttgcc ttgattaacg
     2041 caagggggtt cgtctgtcac aagacgaacc ccttgtttat gtgataaaat agcggggcgg
     2101 cgcgttggaa acagcagcca ggcccgccgc caggggctgc gaagggagtg ctatgactgg
     2161 tattctcgtc cggtgggcga tcaccattat cgcggtattc gtcgcggcca agctcctgcc
     2221 caccatgatc aactacgacg actggctggg cctcgccatc ttcgcggcgg tcctgggcct
     2281 ggtcaacgcc atcgtcaagc ccatcgtcgc catcttgtcg ttgccggtgg tgctgctcac
     2341 cctcggcctg ttcctgctgg ttatcaacgc gttgatgttc tggctggccg cctcgctggt
     2401 gcccggcttc tatgtcaacg gcttctgggg cgccctggtc ggctcgatca tcgtcagcct
     2461 ggtaagctgg gtcctgtcgg cgctgctgcc caactaacca aagatccccc tatccgttgt
     2521 cactctgagc gcagcgaaga gtctcggcgg tggggcacga gacgagatgc ttcactgcgt
     2581 tcagcatgac atgagaggct gagttctcgg cccgctacat cctgatctgg agagaggaaa
     2641 cgtcctgatg ccgcgtcttc ccctgctcgc tcgcgggctg ctgctggtgg tgctgcaacc
     2701 ggcggcggtc gccctggccg ccggtccggc ggccaccacg gtccccgcgc ccgatcccgc
     2761 cttcgcccat gtctgggcgc gcaccgacga actggtgcgc gcgcatgatg tcactcgtag
     2821 ctggtactgg ggcgaggcgc cctttgcccg gctgctggag cctttcgccg gggtggcttc
     2881 gggcacgcgg ctggtcgaat actacgacaa gggccgcatg gagatcaaca acccggcggg
     2941 cgaccggcgc agcccctggt tcgtaacgag tggcctgctg gtcaaggaac tgatctccgg
     3001 cgagatggcc gtgggtctga cgcagaccga agcccacgcc ccttcgaccc agcccatcgt
     3061 cggcgattgg acgccggaa
//
LOCUS       DASUYC010001021         2064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1559062, whole genome shotgun sequence.
ACCESSION   DASUYC010001021 DASUYC010000000
VERSION     DASUYC010001021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1559062"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VF276_16715"
     CDS             <1..449
                     /locus_tag="VF276_16715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608546.1"
                     /translation="LAQGLALYGEENNTPGIVWALDEQARLAQAEGDLHRAGALWVEA
                     LGLCRAARLDRWVVGCLRGLAGVALQQGQIARAVRLQGACAALLQMAGGPPLILPPAE
                     RAAEEAALAAARAQLDAAAWEAAWAAGQAMSLDEAVAYALEGLSPR"
     gene            complement(602..>2064)
                     /locus_tag="VF276_16720"
     CDS             complement(602..>2064)
                     /locus_tag="VF276_16720"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX6608547.1"
                     /translation="AVLDLLNDHLTEPGYIISQLCSGLVEVSPDMEVIPAIARSWEIL
                     DEGRRYVFHLDESARWSDGHPLTAADFELAWRLILAPATAAFLAPDYYVIRGARAYHQ
                     GGAEAPAIRATAEHTLEIELEAPATYFLELVSTVVPVPGHCLEAYGQEWLAPAPLVTN
                     GAFRLEHWEHGRSMTLVRNPYCRLPTAGNIERIELAFNPLEDWQGSLARYEADALDVL
                     DIGAIPTPAAQTIKGRHADEYRSWPSRGLYVLFLDCNRPPFDHPGVRRAFVHAIDREA
                     LAYESLGGADSPATGGLVPPSLRAHSPGIGLAYDPDLARRLLAEAGYPGGRGLPRIRC
                     AVEYDRTAPAIGAYLQQQWAAVLGVESQWTDAGPAHVEGRAGNDEMQVTLIGVAWIYA
                     DPDTCMRLIPMTLGHLSHWQDPDYDRLVDESTRTLDRPRRFALLQRADRILMEGAAVV
                     PLVYPRLHLLVKPWVQRFISFADFPPHLRHVVIEPH"
BASE COUNT          306 a          677 c          775 g          306 t
ORIGIN      
        1 cgctggcgca gggcctggcc ctctacggcg aagagaataa tacaccgggg atcgtgtggg
       61 ccctcgacga gcaagcccgg ctggcccagg cggaagggga tctgcaccgc gcgggtgcgc
      121 tctgggtcga agctctcggg ctctgccggg ccgcccggct tgaccggtgg gtcgtcgggt
      181 gcttgcgagg tctggccggc gtggccttgc agcaagggca gatcgcgcgg gcggttcggc
      241 tgcagggtgc ctgcgcggcc ctcttgcaaa tggccggcgg cccccccttg atcctgccgc
      301 cggcggaacg ggcggccgag gaggcggccc tggccgccgc ccgcgcccaa ctggatgccg
      361 cggcgtggga agcggcctgg gcggccgggc aggcgatgtc gttggacgag gcggtagcat
      421 acgccctgga ggggctgtcg ccccggtaaa tcagcggccc gccggcagcc ggcgggacgt
      481 agcaggccag gcggcggcag gccggcaacc caggcccgcc ggtcatcggc cggtacgatt
      541 ttgcgctgta ggggccggta tcaaaccggc ttggctccat cagaatcagg gcagggggcc
      601 gttagtgcgg ctcgatcacc acgtggcgca gatgcggggg gaagtcggcg aagctgatga
      661 agcgctgcac ccacggctta accagcaggt gcaggcgcgg gtacaccagg gggacgacgg
      721 cggcgccttc catcaggatg cggtcggcac gctgcagcag cgcaaaccgc cgcggccggt
      781 ccagcgtgcg cgtgctttcg tcgaccagac ggtcatagtc ggggtcctgc cagtggctga
      841 gatgccccag cgtcatgggg atcaggcgca tgcaagtgtc gggatccgcg tagatccagg
      901 ccactccgat gagtgtcacc tgcatctcgt cgttcccggc gcggccttcc acgtgcgccg
      961 ggccggcgtc cgtccactgg ctctcgacgc ccagcacggc agcccattgc tgctgtagat
     1021 aggctccgat cgccggggcg gtccggtcgt actccacggc gcagcggatc cgcggcaggc
     1081 cccgcccgcc tggatagccg gcctcggcca gcagccggcg ggccaggtcg gggtcgtagg
     1141 ccaggccgat gcccggcgag tgagcccgca ggctgggcgg caccaatccc cctgtggccg
     1201 gggagtcggc gccgcccagg ctctcgtagg ccagcgcctc gcggtcgatc gcgtggacga
     1261 aggcacggcg cacgccgggg tggtcgaaag gcgggcggtt gcaatcgagg aacaggacgt
     1321 acagcccccg gctgggccac gaccggtatt catcggcgtg gcggcctttg atcgtttgcg
     1381 ccgccggggt ggggatggcg ccgatatcga ggacatccag ggcatcggcc tcgtaacggg
     1441 ccaggctgcc ctgccagtct tcgaggggat tgaaggccag ttcgatgcgc tcgatgttgc
     1501 ccgcggtggg caggcggcag tatggattgc ggaccagggt catcgaccgg ccgtgctccc
     1561 agtgttccag gcggaacgcg ccgttggtca ccagcggtgc tggggccagc cactcctgac
     1621 cgtaggcttc caggcagtga cccggcaccg gcaccacagt ggacaccagt tccaggaagt
     1681 aggtggcggg cgcctccagt tcgatctcta gcgtgtgctc ggcggtggcc cggatcgccg
     1741 gcgcctcggc cccgccctgg tggtaggccc gcgccccgcg aatcacataa tagtcggggg
     1801 ccaggaacgc ggcggtggcc ggcgccagga ttaagcgcca ggccagctca aagtctgccg
     1861 ccgttagcgg gtggccatcg ctccagcgcg cggactcgtc caggtggaag acgtagcggc
     1921 ggccctcgtc caggatctcc caggagcgcg ctatggccgg gatgacctcc atgtccgggc
     1981 tgacttccac cagcccactg caaagctgcg agataatgta accgggctcg gttaagtggt
     2041 cgttcagcag gtcgaggacg gccg
//
LOCUS       DASUYC010001022         2101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_2039664, whole genome shotgun sequence.
ACCESSION   DASUYC010001022 DASUYC010000000
VERSION     DASUYC010001022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2039664"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VF276_16725"
     CDS             <1..468
                     /locus_tag="VF276_16725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608548.1"
                     /translation="EVNPTDGKTYQVQWFQRARFEYHPETAPPDDVQFGLLGNIAAAG
                     RRDEPPFRPVARPAALSAALSWFPETEHTLQGPFLDFWTRYGSWKQFGFPISEMFTEP
                     SKIDGKPYVVQYFERARFELHPGLAPEFGGVVLGQLGTEQYHPPAATPTPAQP"
     gene            487..987
                     /locus_tag="VF276_16730"
     CDS             487..987
                     /locus_tag="VF276_16730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608549.1"
                     /translation="MHGQAYHRKVIIPHLNNALEEYLWLASQVPVALLDTTPAGASLE
                     AGPPVRRQVLDLALELDTVVYPALIALLNGRDGPPALASDEIAARRASWNAADLMVAM
                     KQIRYRFQATEALLQDTVDIDWSTPSGPTPLALAAFALWRRLLRALDTLGGTVAALEP
                     GAPAAS"
     gene            1098..>2101
                     /locus_tag="VF276_16735"
     CDS             1098..>2101
                     /locus_tag="VF276_16735"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HEX6608550.1"
                     /translation="MDDVAIGLDDLPEDDSRDLGDDLDLSGAPHGYRDWRNDPQLPRR
                     TDIPGYPNGFYTRRKQPVSILCVHHTAGANDDIFFDNTYHTQQRNWNDPGEPMLHAPH
                     IAYHFYIDRDGLLTGCNFLWERSWHATNANDKAIGIVCQGDFQSGDNRPTDAQRETLQ
                     WIISRLLGYFKLDRQAIWGHGELRQYGNSTTCPGKNFLPDVQGFRGGKDFWTAPAPAP
                     GPAPQPDTTDPAEIHWRVMDPNGGQLGLFSQSGTAAAGAEQVSGLGIYLGNGEIRIDF
                     RGAQNANSNAPAKWRVCDANNVHVGLYNQTPNAVDHAERIQGYALYLYDSADGTIRLD
                     "
BASE COUNT          367 a          759 c          664 g          311 t
ORIGIN      
        1 gaagtgaacc cgaccgacgg caagacgtac caggtgcagt ggttccagcg ggcgcgcttc
       61 gagtaccacc cggagacagc cccgccggac gacgtgcagt tcgggttgct gggcaacatc
      121 gcggcggccg gccggcgcga cgagccgcct ttccgcccgg tggcccggcc cgcggccctg
      181 agtgccgcgc taagctggtt cccggagacc gagcacacgc tccaggggcc attcctcgac
      241 ttctggaccc gctacggcag ttggaagcaa ttcggcttcc cgatttctga gatgtttacc
      301 gagccaagca agatcgacgg caagccctat gtcgtccagt atttcgagcg ggcgcgcttc
      361 gagttgcatc cggggctggc gccggagttc ggcggcgtgg tgctgggcca actgggcacc
      421 gagcagtatc acccgcccgc cgcgaccccg acgccggccc agccctaacc gccaaggaga
      481 ccgccgatgc atggtcaggc ctatcaccgc aaggtcatca tcccccacct gaacaacgcg
      541 ctggaggagt acctctggct ggcctcgcag gtgccggtcg ccttgctcga taccaccccc
      601 gccggtgcct ctttagaggc agggccgccc gtgcgccgcc aggtgctgga cctggccctc
      661 gaactggaca cggtggtcta cccggccctg atcgcgctgc tcaacgggcg agacgggccc
      721 ccggcgctgg cctccgatga gatcgccgcc cggcgcgcct cctggaacgc cgccgacctg
      781 atggtcgcca tgaagcagat ccgctatcgc ttccaggcca cggaggcgct gttacaggac
      841 acggtggaca tcgactggag tactccgtcc gggccgacgc cgctggcgct ggcggcgttc
      901 gcgctctggc ggcggctgct gcgcgccctc gacaccctgg gcggcaccgt ggcggcgctg
      961 gaacccggcg cgcccgcggc atcgtaacgc gcgctaacgc ccgataatcg gcctattcgg
     1021 cccggcgtgc ccggtgcgcg ccgggatggg agtctcgtgg tggttcagtt tcttagctgg
     1081 taaaggagag cctggcgatg gatgatgttg caatcggcct ggatgatctg ccggaggacg
     1141 atagccgcga cctgggcgac gatctcgacc tgagcggggc gccgcacggc taccgcgact
     1201 ggcgcaacga cccgcaactg ccgcgccgga ccgatattcc cggctacccg aacggcttct
     1261 acacgcgccg caagcagccg gtgagcatcc tgtgtgtcca ccacacggcc ggcgccaacg
     1321 acgacatctt cttcgacaac acctaccata cacagcagcg caactggaac gaccccggcg
     1381 agcccatgct gcacgcgccc catatcgcct accacttcta catcgaccgc gacggtctgc
     1441 taacgggctg caacttcctc tgggaacgct catggcacgc gaccaacgcg aacgacaagg
     1501 cgatcggcat tgtctgccag ggcgatttcc agagcggcga caaccgcccc accgacgccc
     1561 agcgcgagac cttgcagtgg atcatcagcc gcctgctggg ctacttcaag ctggaccggc
     1621 aggccatctg gggccatggc gaactgcggc aatacggtaa ctcgaccacc tgccccggca
     1681 agaactttct gcccgacgtg cagggcttcc gcggcggcaa ggacttctgg accgcgcccg
     1741 ccccggcccc tggcccggcc ccccagccag acaccaccga cccggcggag atccactggc
     1801 gggtgatgga tcccaacggt gggcagttgg ggctcttcag ccagagcggc accgcagcgg
     1861 cgggcgccga gcaggtgagc gggctgggca tctacctggg caacggcgag atccgcatcg
     1921 actttcgcgg tgcgcaaaac gccaactcca acgccccggc caaatggcgc gtctgcgacg
     1981 ccaacaacgt ccatgtgggc ctctacaacc agacacccaa cgccgtcgac cacgccgagc
     2041 gcatccaggg ctacgcgctc tacctgtacg acagcgccga cggcacaatc cgcctcgatt
     2101 t
//
LOCUS       DASUYC010001023         2231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1333166, whole genome shotgun sequence.
ACCESSION   DASUYC010001023 DASUYC010000000
VERSION     DASUYC010001023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333166"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VF276_16740"
     CDS             <1..327
                     /locus_tag="VF276_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX6608551.1"
                     /translation="GLRVDGEVYGDIQAMPEDAGSMLVISEASVVQGAIQADHVIING
                     TVRGPVHARELLELQPKARIEGDVSYVALEMHQGATICGQLRPLVASAAGEEEKPLLK
                     LAAKSA"
     gene            388..762
                     /gene="erpA"
                     /locus_tag="VF276_16745"
     CDS             388..762
                     /gene="erpA"
                     /locus_tag="VF276_16745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEX6608552.1"
                     /translation="MSAVAENLTAEMPAPLLFTDSAAAKVAELIAEEGNPDLKLRVFV
                     QGGGCSGFQYGFTFDEIANDDDTVMTKNGVQLLIDAMSFQYLVGAEIDYKEDLQGAQF
                     VIKNPNAQTTCGCGSSFSPSDH"
     gene            complement(759..1865)
                     /locus_tag="VF276_16750"
     CDS             complement(759..1865)
                     /locus_tag="VF276_16750"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEX6608553.1"
                     /translation="MPDLFIGLMSGTSMDGVDGVIADFSSGVRVLQHASAPFAEPLRA
                     ELMALNASGPDELHRAWLAGNALMRVYADVVGQLLAQSGRSPADIAAIGAHGQTVRHR
                     PQMFDGTGYTIQLAQPALLAELAGIRVVADFRSRDVAAGGQGAPLAPFFHQAVFARPG
                     ETVGVLNIGGISNITLLRADGSMQGFDCGPGNALMDAWCAEHTGRPYDDDGRWAASGR
                     VLPDLLASLLAEPYFEQPPPKSTGRDLFNRPWLAAHLQGLAAAAPQDVQATLTELTAR
                     ACAGDAARHAPGLLRLVICGGGALNGHLMGRLQALLPDTQVVSSAQAGLPPLQVEAAA
                     FAWLAMKCLRNEKLELQSTTGAKGARVLGGIYPA"
BASE COUNT          369 a          764 c          780 g          318 t
ORIGIN      
        1 ggcctgcggg tcgacggcga ggtctacggc gacatccagg ccatgccgga ggacgccggc
       61 agcatgctgg tgatctccga agcctcggtg gtccagggcg ccatccaggc cgaccacgtg
      121 atcatcaacg gcaccgtgcg cggcccggtc cacgcccgcg agctgctgga actgcagccc
      181 aaggcccgga tcgagggcga cgtgtcctat gtcgcgcttg agatgcacca gggcgccacc
      241 atctgcggac agttgcggcc gctggtcgcc tccgccgccg gcgaggagga aaagccctta
      301 ctcaagctgg cagcaaagag cgcctgatcg acgggccccc aggggcccgc gtaatatgat
      361 ggtgccgcac ctgacgaaag agagcccatg agcgctgttg ccgaaaacct gaccgccgaa
      421 atgcccgccc cgctgctgtt caccgacagc gccgccgcca aggtcgccga gctgatcgcg
      481 gaagagggca accccgacct gaagctgcgc gtgttcgtgc agggcggggg ctgctcgggc
      541 ttccagtacg gcttcacctt cgacgagatc gccaacgacg acgataccgt catgaccaag
      601 aacggggtgc agctgctgat cgacgcgatg agcttccagt acctggtcgg cgccgagatc
      661 gactacaagg aagacctgca gggcgcccag ttcgtcatca agaacccgaa cgcgcagacc
      721 acctgcggct gcggctcgag cttctcgcct tccgaccact aggccgggta gattcccccc
      781 agtacacggg cgcctttggc gcccgtggtg ctttgcagct ccagcttttc gttgcgcagg
      841 cacttcatgg ccagccaggc gaaggcggcc gcctccacct gcaggggcgg caggccggcc
      901 tgggcactgg agaccacctg tgtatcgggc aacaacgcct gcagccggcc catcaggtgg
      961 ccgttgagcg ccccgccgcc gcagatgacc agccgcagca agcccggcgc atgccgggcg
     1021 gcgtcgccgg cgcaggcacg tgcggtcagt tcggtcagcg tcgcctgcac gtcctgcggc
     1081 gcggccgcgg ccaagccctg caggtgcgcc gccagccagg gccggttgaa caggtcgcgg
     1141 ccggtgctct tgggcggcgg ctgctcgaag tacggctcgg ccagcagcga ggccagcagg
     1201 tccggaagca cgcgcccact tgccgcccaa cggccgtcgt cgtcgtaagg ccggccggtg
     1261 tgctcggcgc accaggcgtc catcagcgcg ttgccggggc cgcagtcgaa gccctgcatg
     1321 gagccgtcgg cgcgcagcag ggtgatgttg gagatgccgc cgatgttcag tacgcccacc
     1381 gtctcgccgg gacgggcaaa cacggcctgg tggaagaacg gcgccagcgg cgcgccctgg
     1441 ccgccggcgg cgacgtcgcg gctgcggaag tccgccacga cgcggatgcc cgccagctcc
     1501 gccagcaggg cgggctgggc gagctggatg gtgtagccgg tgccgtcgaa catctgcggc
     1561 cggtggcgca cggtctggcc gtgggcgccg atcgcggcga tgtccgcggg gctgcggccc
     1621 gactgcgcca gcaactggcc caccacgtcc gcatagacgc gcatcagcgc attgccggcc
     1681 agccaagccc ggtgcagttc gtcggggccg gaggcgttga gcgccatcag ctcggcgcgc
     1741 aggggctcgg cgaaaggggc actggcgtgc tgcagcacgc gcacgcccga ggagaaatcg
     1801 gcgatgacgc cgtccacccc gtccatcgag gtgcccgaca tcaggccgat gaagagatcc
     1861 ggcatggggc ggatgctatg ggaactctga aaaatccgct gcgcgaattt ttcagagttg
     1921 tctgatcagt gcggccagga ccggcaaagc cggatcctgt ccgccgggcg agaaccgcgc
     1981 ggaataacat gcagcgcggc tccccttgtg agaaggatcc ggcttcgccg gcccttctca
     2041 cacatgctga gcctcagaaa aaagtcgttc acgacctttt ttctgagatt cgctaccgcc
     2101 cccgcggcgg ttcagcgcgc ggcagcgacc atgttggccg tggcggccac ggccagctgg
     2161 gtgcgcatgc tgcgtgccag ccggtcgaag tcgacgcggg cctgggccgt cagctcgggg
     2221 cgcggcgcct g
//
LOCUS       DASUYC010001024         2562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_2021712, whole genome shotgun sequence.
ACCESSION   DASUYC010001024 DASUYC010000000
VERSION     DASUYC010001024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2021712"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..959
                     /locus_tag="VF276_16755"
     CDS             3..959
                     /locus_tag="VF276_16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX6608554.1"
                     /translation="MLGLWALVVLFPLYWLVVTSFKTPIEVDSGPFYLPYVDFQPTLD
                     AWQYIFGTVWNDTLRPYINTVVVGLSSATLALVLGSTAAYALVRFQYQPRLGAIGLFI
                     GCVLLGTAAIIAGVPWQIAVLSALACFVLLLQTIGRRFKRALGNNDIAFWLISQRMLP
                     PVAVVIPVYVLFQRFGLLDTQAALIITYVATNLPIVVWLMRDYFQTIPVELEECAAID
                     GASTYRIFWSIVLPIAVPGLVATFLFVLVFAWNEYLLALFLSNANAQTMPLTVAAQNA
                     TRGPQWWYMSVLILIMIIPVIAMAVVLERYISRGLLVGAVKG"
     gene            985..1215
                     /locus_tag="VF276_16760"
     CDS             985..1215
                     /locus_tag="VF276_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019424050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608555.1"
                     /translation="MTWEYHTTRDDASLTTLGLQGWELVAVLPGKGQEASPTYYFKRP
                     APDLREEVTGAQKRRYYAQLGHALPDAEEVKD"
     gene            1212..1598
                     /gene="rbsD"
                     /locus_tag="VF276_16765"
     CDS             1212..1598
                     /gene="rbsD"
                     /locus_tag="VF276_16765"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019424049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEX6608556.1"
                     /translation="MKKGGILHPEICKLLAASGHTDYITICDRGFPVPEGPSRIDLAL
                     VDNIPTVLDVLRAVAAEWTLDRVIIAQEMTEVSPQHVEAIRAIVGDVPLDVRPHLELK
                     HLSRTARGTIRTGDTTPWGNIVIVSG"
     gene            1615..2466
                     /locus_tag="VF276_16770"
     CDS             1615..2466
                     /locus_tag="VF276_16770"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX6608557.1"
                     /translation="MTEDGERGLERITPEAIVRAAGLVRRGQVYDLGAELSDDMPETS
                     KDAFMPYRLLTYRTNRDMARDVDWHGVSFYTEVLMATPHVSTHIDALNHVSQDGRIYG
                     GHRLEDVERDFGMAAEGIETVPPIVSRGVLLDVAAHKGVAQLADHYEIAVADLEGTLA
                     RQGTVLQRGDTVLVHTGKMRQYAVDNAAFLAGQPGVGVAAAVWLYEQGMAVLGSDTTG
                     TEPQPVTDWTQTVHVAMLRDRGVHLIEWMYLEDLAAAHAYEFLFICLPLKLRGASGSW
                     VRPVAVV"
BASE COUNT          422 a          802 c          896 g          442 t
ORIGIN      
        1 tgctgctggg gttgtgggcg ctggtcgtcc tgttcccgct gtactggctc gtggttacgt
       61 cgttcaaaac gcccatcgag gtggacagcg ggcccttcta cctgccgtac gtcgattttc
      121 agcccacgct ggacgcctgg cagtatatct tcggcaccgt ctggaacgat acgctgcggc
      181 cctacatcaa cacggtcgtg gtcggcctga gcagcgccac gctcgcgctg gtcctcggat
      241 cgacggctgc ctatgccctg gtgcgcttcc agtaccaacc gcggctgggc gccatcgggc
      301 tgtttatcgg ctgcgtgctg ctgggcaccg cggccatcat tgccggggtg ccctggcaga
      361 tcgccgtctt gtccgcgctc gcatgcttcg tgctgttgct gcaaaccatc gggcggcgct
      421 tcaagcgcgc gctgggcaac aacgacatcg ccttctggct gatctcgcag cgcatgttgc
      481 cgccggtcgc ggtggtgatc ccggtctacg tgctgttcca gcgcttcggc ctgctggaca
      541 cccaggcggc cctgatcatc acctacgtgg cgaccaacct gcccatcgtg gtctggctga
      601 tgcgcgacta cttccagacc atcccggtgg agttggagga gtgcgcggcc atcgacggcg
      661 cgtccaccta ccgcatcttc tggtcgattg tgctgcccat cgccgtgccg ggactggtcg
      721 ccacgttcct gtttgtgctg gtcttcgcct ggaacgaata cttgctggcc ctgttcctgt
      781 ccaacgccaa cgcgcagacg atgccgctga cggtggcggc gcaaaacgcg acgcgcgggc
      841 cgcagtggtg gtatatgtcg gtgctgatcc tgatcatgat catcccggtc atcgccatgg
      901 ccgtggtatt ggagcggtac atatcgcgcg gcttactcgt cggcgccgtg aagggctagc
      961 gtggggcgca aggggggatg cggaatgacc tgggaatacc acacgacacg ggatgacgcg
     1021 agcctgacga ccctggggtt gcagggctgg gaactggtgg ccgtactgcc ggggaagggg
     1081 caggaggcca gcccgactta ctacttcaag cgcccggccc cggacctgcg cgaggaggtg
     1141 acgggggcgc aaaagcggcg ctactatgcc caactgggcc atgccttgcc tgacgccgag
     1201 gaggtaaagg attgaaaaag ggcggcatcc tgcacccgga gatctgcaaa ctgctggccg
     1261 cgtcggggca caccgactac atcaccatct gcgatcgggg ctttccagtg cccgaaggcc
     1321 ccagccggat cgacctggcg ctggtggaca atattccgac cgtgctggac gtgctgcggg
     1381 ccgtggccgc cgaatggacg cttgaccggg tgatcatcgc gcaggagatg accgaggtca
     1441 gcccccagca tgtggaagcg atccgggcca tcgtgggcga cgtgccgctc gacgtgcgcc
     1501 cccacctgga gttgaagcac ctgtcgcgca ccgccagggg caccatccgc acgggcgaca
     1561 cgacgccctg gggcaatatc gtgattgttt cgggctgagg agaggaacag acgcatgacg
     1621 gaagacggag agcgagggct ggagcggatc acgccggagg cgatcgtccg ggccgccggg
     1681 ttggtgcgcc gtgggcaggt ctatgacctg ggggccgaac tcagcgacga catgccggag
     1741 accagcaagg atgccttcat gccctaccgg ctgctgacct accgcacgaa ccgcgacatg
     1801 gcgcgggacg tagactggca cggtgtgtcc ttctacaccg aggtgttgat ggcgacgccc
     1861 catgtctcga cccacatcga cgccctcaac cacgtcagcc aggatggccg catctacggc
     1921 gggcaccgcc tcgaagacgt ggagcgcgat ttcggcatgg cggcggaagg catcgaaacc
     1981 gtgccgccca tcgtgagccg cggcgtgctg ctcgacgtgg ccgcccataa gggcgtcgcg
     2041 caattggcgg accactacga gatcgccgtg gccgacctgg aaggcacgct ggcccggcag
     2101 ggcacggtcc tgcaacgggg cgacacggtg ctggtccaca ccggcaagat gcggcagtac
     2161 gccgtggaca acgcggcgtt cctggccggg cagccggggg tcggcgtggc cgccgcagtc
     2221 tggctgtacg agcagggcat ggccgtgctg ggcagcgaca cgacgggcac cgagccgcaa
     2281 cccgtcaccg attggacaca gaccgtgcat gtggcgatgc tgcgcgaccg gggagtccac
     2341 ctgatcgaat ggatgtacct ggaagacctg gccgcggcgc acgcgtatga gttcttgttt
     2401 atttgcctgc cgctgaagct gcgcggggcc agcggaagct gggtgcggcc cgtggcggtc
     2461 gtgtaggcaa gcggagcggg aggagccgat gagactgcgg ggcaagaccg ctatcgtgac
     2521 cggcggcggt agcgggatcg ggcgggcctc ggcggcgttg tt
//
LOCUS       DASUYC010001025         3097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_310876,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001025 DASUYC010000000
VERSION     DASUYC010001025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310876"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..1712)
                     /locus_tag="VF276_16775"
     CDS             complement(105..1712)
                     /locus_tag="VF276_16775"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX6608558.1"
                     /translation="MDNVDEVLARLDGFIPRKMAAAHTPGLALALTDGDRLLAVRAYG
                     YAGLGAHTPVTPDTLFEIGSQGKTFTAVAVLQQVEAGRIDLDAPVTRYLPWFSVQSVY
                     EPITIHHLLSHTAGIIAGTEFATAGPAEVWALRDTWTGFAPGAHYHYSDVGYKILGLV
                     LEHVLGRPYGDIIAEQILVPLGMGDTEPVLTHALRPRLATGYASLYDDRPMQRTHPLV
                     PAAWVETDTADGSLASTVGDMAAFLRMLIGRGATPQGRILSPMSFDRLTRPVIEMADG
                     LFYGYGIISSRTAGFLRLQHGGDMPGYESSWRIDADNGLGVTVLMNGPVSAGVADFAL
                     KLLRAAYAGQAWPPDPPLPDPSQVADAAEYAGVYRLDDRTLTLRAAGESLTLEQDGTR
                     VPLEQEDGDWFHADHPDWDRFPLTFAREGATIIAAIWGGDYYRRAGHPAPPVPAPPAA
                     WAAYPGHYRSHNPWRSNFRVVLRQGALRLIDPSGDEEPLTELADGLFRVGEAGYSPER
                     LRFDAIVEGQALRANYSGCDYYRFFTP"
     gene            2039..>3097
                     /locus_tag="VF276_16780"
     CDS             2039..>3097
                     /locus_tag="VF276_16780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608559.1"
                     /translation="MMIHRILRRLPVPAVLALLVVGPAGAGVRAEDTVRTFPETGMTV
                     GGAFLQYWQNGGGLAQFGYPISDIFAETSPLNGKNYTVQYFERAVFEAHPENQPPYDV
                     LLSQLGTFRYREKYPDGAPDQHPAAGGRLFPETGHVVGGAFLTYWQTHGGLAAQGYPI
                     SDEFTEVSDLDGKSYTVQYFERAVFEAHAENQPPYDVLLSQLGRFGYEQQYQVPPPIA
                     GTEPGGSPTPAAAPPTATATPAPGSADWPMYGHDAARTNYNPGETVLSEENVGRLAPG
                     WQADLGSRGTPPSGGPIVAGGRVYAGSSAPSGPNYFAFDARTGTRLWGANLNYMHSDC
                     DDVGIGATAAISGSILVAG"
BASE COUNT          497 a         1113 c         1051 g          436 t
ORIGIN      
        1 tttgcgctgt aggggccggt ttcaaaccgg cctgtccccg gcgcgctgca ggggccggtt
       61 tcaaaccggc cttgccgctc cacagaattg ccggcctgga accgctatgg tgtgaagaag
      121 cggtagtaat cgcagcccga gtagttggcc cgcaacgcct ggccctcgac aatcgcgtcg
      181 aagcgcagcc gctccggcga gtacccggcc tcgcccaccc ggaacagccc gtcggccagt
      241 tccgtcagcg gctcttcatc gccggatggg tcgatcagcc gcagcgcacc ctggcgcagc
      301 accacccgaa agttcgaccg ccaggggtta tgcgaccggt agtggccggg atacgcggcc
      361 caggcggcgg gcggcgccgg cacgggcggg gccgggtgcc ccgcgcgccg gtaataatcg
      421 ccgccccaga tcgccgcgat gatggtggct ccttcccgcg caaaggtcag cgggaagcga
      481 tcccaatccg ggtggtccgc atggaaccag tcgccatctt cctgttctaa cggcacccgc
      541 gtgccgtcct gctcaagggt tagcgattcg ccggcagcac gcagcgtcag ggtgcggtcg
      601 tccagccggt atacgcccgc gtactcggcg gcgtccgcca cctgggaggg atcgggcagc
      661 ggcgggtcgg gcggccaggc ttgcccggca taggcggcgc gcagcagttt gagggcaaaa
      721 tcggccacgc ccgcgctcac cgggccattc atcaacacgg tcacgcccag gccgttgtcg
      781 gcgtcgatgc gccacgacga ctcatagccc ggcatatcgc cgccgtgttg cagccgcaga
      841 aagcccgccg tccggctgga aatgatgccg tagccgtaga acagcccgtc cgccatctcg
      901 atcaccgggc gggtgagccg atcaaagctc atcggcgaca ggatgcgccc ctgtggcgtg
      961 gcgccccgcc cgatgagcat gcgcaggaac gccgccatat cgcccaccgt ggaggccagc
     1021 gacccgtcgg cggtgtcggt ctcgacccag gcggcgggga ccagcgggtg agtgcgctgc
     1081 atcgggcggt cgtcgtacag cgacgcgtag cccgtcgcca gccgggggcg cagcgcgtgg
     1141 gtcaacacgg gctcggtatc gcccatgccc agcggcacca ggatctgctc ggcgatgatg
     1201 tcgccgtagg gccggcccag cacatgctcc agcaccaggc ccagaatctt gtagcccacg
     1261 tccgagtaat ggtaatgggc gccgggcgcg aagccggtcc aggtgtcgcg caaggcccag
     1321 acttccgccg gtcccgccgt ggcgaactcg gtgcccgcga taatgcccgc cgtgtggctc
     1381 agcaggtggt ggatggtgat cggctcatag accgactgca cgctgaacca gggcaggtag
     1441 cgggtcaccg gcgcgtcgag gtcgatgcgc ccggcctcca cctgctgcaa gaccgcgacg
     1501 gcagtgaagg tcttgccctg cgagccgatc tcgaacaggg tgtccggcgt cacgggggta
     1561 tgggcgccga ggccggcgta gccgtaggcg cgcaccgcca gcagccggtc gccgtcggtc
     1621 agcgccaggg ccagacccgg cgtgtgcgcc gccgccatct tgcgcgggat gaagccgtcg
     1681 agccgggcca gcacctcgtc gacattgtcc atcacaccca cctcccatcc tggcccgccc
     1741 cgcagcacat gccgccgggc cgcgccaacc gcagtatact ccgcccggcg gcgcggcaca
     1801 acggagccgc aggctattcg gcgggcctgt ccgaggttgc gaatgagcgc cggagattgc
     1861 ccgcctcggc aaaacctgct aagataggcc catcgccccg gccaagcggc agcgccgcga
     1921 acctcaatcc tttcggcgtg gcgccacccg ccggttgcga tcgcgatccc tacgcgggat
     1981 gctccccttg caccatcaac gcgaggcgac gcgcaatcca ggaggtcgat cctacttcat
     2041 gatgatacac cgtatcttgc gccggctgcc ggtgccggcc gtgctggcgt tgctggtcgt
     2101 cgggccggcg ggggccgggg tacgcgcgga agacaccgtc cgcaccttcc cggaaaccgg
     2161 catgacggtc ggcggcgcgt ttttgcagta ctggcagaac gggggcggcc tggcccagtt
     2221 cggctatccc atcagcgaca tcttcgctga aaccagcccg ctgaacggga aaaactacac
     2281 tgtgcaatac ttcgagcggg ccgtgttcga ggcccacccg gaaaaccagc cgccctacga
     2341 tgtgctgctg tcccagctcg gcaccttccg ctaccgcgag aagtacccgg acggcgcgcc
     2401 ggaccagcac cccgccgccg ggggccgcct gttcccggag accgggcacg tcgtgggcgg
     2461 cgccttcctg acctactggc agacgcatgg cgggctggcc gcgcagggct acccgatcag
     2521 cgacgagttc accgaggtca gcgatctcga cggcaaatcc tacacggtgc agtatttcga
     2581 gcgggccgtg tttgaggccc acgccgagaa ccagccgccc tacgacgtgc tactgtcgca
     2641 actgggccgc ttcggctacg agcagcagta ccaggtgccg ccgccgatcg ccggcaccga
     2701 gccgggcggc tcgcccacgc ccgccgcggc gccgcccacc gccaccgcca ctcccgcgcc
     2761 gggcagcgcg gactggccca tgtacggcca cgacgcggcg cggaccaact ataaccccgg
     2821 cgagacagtg ctcagcgagg agaacgtggg ccggctggcg cccggctggc aggcggacct
     2881 gggcagccgc ggcacgcccc cctccggcgg accgattgtg gccggcgggc gcgtctatgc
     2941 gggcagcagc gcgcccagcg gccccaacta ttttgccttc gacgctcgca ccggcacgcg
     3001 cctctggggc gcgaacctga actacatgca cagcgactgc gacgacgtgg gcatcggggc
     3061 caccgcggcc atctcgggta gcatcctggt ggccggg
//
LOCUS       DASUYC010001026         4755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1828367, whole genome shotgun sequence.
ACCESSION   DASUYC010001026 DASUYC010000000
VERSION     DASUYC010001026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1828367"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VF276_16785"
     CDS             complement(<1..656)
                     /locus_tag="VF276_16785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608560.1"
                     /translation="MPGRIGLTIDTNDLTLAVGERALVSCVVTNQGDVVDAFTLAIPA
                     LSPDWYTLTPAQVSLFPQTQALVMLEIHPPPTGTTLAGDYPFQLVVVSRDQPDERMEL
                     TITLHIAAVSELAVALEPQRIVARQGTFQVRLTNNGNVDRPVVVRPSDPDARLVFTFG
                     AAEALPAGATTTPLSAGADPATPADPNVAVAQPVGTLAPVGTEVLTEWTAPVAGVDQG
                     R"
     gene            complement(660..1817)
                     /locus_tag="VF276_16790"
     CDS             complement(660..1817)
                     /locus_tag="VF276_16790"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HEX6608561.1"
                     /translation="MDDAVPRVQLKRVDPFLGLMIDAETWRDSHDYHRQAEAAHALAL
                     HGWGIVAGLEVDAADPADRSVWIRPGVALDPEGRLIIVAQPFRYQINTQEAGAVFLVL
                     MFREIPTQPALSVEDGKERPSRLLEAYAIYERDRLPDQPHVELARLQLSAGRKPVRNA
                     ADATAPKPDEIDTRFREQAGIRPGPRATVGVWKPGEATEKLLPHLAGPTRLLSELSGH
                     IDWRLRRRDQPHGDDPIDCDLLIMPVHTQAAPTEAERRQLTTFLDGGGVLLAEHCVAI
                     GGEAQRAVVAIAQAVGRQPQPVARNHPLLTARHVFSAPPPGAGAGQIYEAGGLILSGV
                     DYCCAWNGGTPEKALDRADIRNAVEFAENLLAYALARRAEVRRAQRSGPRR"
     gene            complement(1823..2506)
                     /locus_tag="VF276_16795"
     CDS             complement(1823..2506)
                     /locus_tag="VF276_16795"
                     /inference="COORDINATES: protein motif:HMM:NF021220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEX6608562.1"
                     /translation="MSPPQDLSALAATVPDAPRTISSNGHGPMPLGFADGKTPEPSSY
                     LRLLPAIYAADEFAGRFLRIFEDVFDPISVMVDNLPYYFDPMTAPVELLDWLAMWVDL
                     HEGEEWPLPRRRALIAAAAALYRMRGTRAGIKQHVGIYTGGLPLVMERTNGFRLDPDA
                     RLGLNTSIGENRPQTFTVVVAVPRPDELDMDTLRGIIEADKPVETTYVLRVVKLDLPA
                     GALSMRKRR"
     gene            complement(2503..4464)
                     /locus_tag="VF276_16800"
     CDS             complement(2503..4464)
                     /locus_tag="VF276_16800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HEX6608563.1"
                     /translation="MVLPVPKLDDRSFQDLVNDAKRLIPRYCPEWTDHNVSDPGVTLI
                     ELFAYMTDILLYRINRVPLRNYIKWLEMLGVKLEPPRPARADITFYLTGPQPEAVTIP
                     AGSQVATVRTESQEAITFSTDQDLTIYVPTLSGLRVNRDGYTYQDYMPVLRTPALNMG
                     IFQDPPQPNDAMYFGYYEDLRGHILRLDVDSQIEGIGVNPKDPPLAWEYWDGPAQEWG
                     ALYLESDTTGGLNRPGEVVVHVPFGARPRDIDGHVAFWIRCRATAPRPNQPAYSAAPR
                     IREVNTVALGGLVPASQVEFIGQEVLGRATGLPGQEWQLSHPPVLARDDGEFLEVEQE
                     DGSFLGWQEVEDFGASRPGDAHYMLDSLTGVIRFGPLIRDSGGREIQYGAHPVEGRLL
                     RFTGYRTGGGTRGNVGKNTITVLKSSIPYIATVANARPAIGGEDAETIDMALLRGPQL
                     LRSRSRAVTAEDYEYLAKRATPEVLRARCLAPSPTEVAAGRAIIRVLLVPASAHTDSA
                     IPPPELALSDRARGEVMTYLNERRLITSTLQIDPPEYRFVSVEVTAGARKRALRDGLR
                     GEIERALYRFINPVHGGPDGEGWPWERNLFQSEVTALVHGIEGVEYVESVRLYVVDMI
                     TNTRTPVEGTITCPANGLLASYNHVVTIK"
     gene            complement(4471..>4755)
                     /locus_tag="VF276_16805"
     CDS             complement(4471..>4755)
                     /locus_tag="VF276_16805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008184152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HEX6608564.1"
                     /translation="ILSTAKGERHMRPQFGCGIHELVFAPNNATTAGLVESYVEEAIG
                     WWEPRVEVLEVNVDTDDDERNLLIITIHYRVRATNDERNLVYPFYLIRSE"
BASE COUNT          680 a         1522 c         1788 g          765 t
ORIGIN      
        1 cgcccctggt ccacgcccgc gaccggcgcg gtccactcgg tcagcacttc ggtgccgacc
       61 ggcgccagcg tgccgaccgg ttgggcgacg gccacgttcg ggtcggcggg cgtggcgggg
      121 tcggccccgg cgctcagggg cgtggtggtg gccccggcag gcagagcctc ggcggcgccg
      181 aaggtgaaga ccaggcgggc gtccgggtcg ctgggccgca cgacgacggg ccggtccacg
      241 ttgccgttgt tggtcaggcg cacctggaac gtgccctggc gggccacgat gcgctgcggc
      301 tccagggcca cggccagttc gctgaccgcg gcgatgtgca gcgtaatggt cagttccatg
      361 cgttcgtcgg gctggtcgcg cgagacgacg accaactgga acgggtaatc gccggccagc
      421 gtggtgccgg tgggcggcgg gtggatctcc agcatgacca gggcctgcgt ctgcgggaag
      481 agcgagacct gggccggcgt cagggtatac cagtcgggac tcagcgccgg gatagccagg
      541 gtgaaggcgt cgaccacatc gccctggttc gtgacgacgc aggacacgag ggcgcgctcg
      601 cccacggcca gcgtgaggtc gttagtatca atggtcaggc caatacgccc cggcatgaat
      661 tatctcctcg gcccactacg ctgcgcccgg cgcacctcgg cccggcgggc cagcgcgtaa
      721 gccagcaaat tctccgcgaa ttcgaccgcg ttgcggatgt cggcgcggtc gagcgccttc
      781 tccggcgtgc cgccgttcca ggcgcagcaa taatcgacgc cgctcaggat cagtccgccg
      841 gcctcgtaga tctgcccggc gcccgcgccc ggcggcgggg ctgagaagac gtggcgcgcg
      901 gtcagcagcg ggtggttgcg cgccaccggc tgcggctggc ggcccaccgc ctgggcgatg
      961 gcgaccacgg cgcgttgcgc ctcgccgccg atggcgacac aatgctcggc cagcagcacc
     1021 ccgccgccgt cgaggaaggt ggtcaactgg cggcgctcgg cctcggtcgg ggcggcctgg
     1081 gtatgcacgg gcatgatcag caggtcgcag tcgatggggt cgtcgccgtg cggctggtcg
     1141 cggcggcgca accgccagtc gatatggccg ctcaactcgc tcagcaggcg ggtggggccg
     1201 gccaggtgcg gcagcagctt ctccgtggct tcgcccggct tccagacgcc gaccgtggcg
     1261 cgcgggccgg ggcgaatgcc cgcctgctcg cggaagcggg tgtcgatttc gtcgggcttg
     1321 ggcgccgtgg cgtcggcggc gttgcgcacc ggtttgcgcc cggcggagag ttgcaggcgg
     1381 gccagttcca cgtgcggttg gtcgggcagg cggtcgcgct cgtagatggc gtaggcttcg
     1441 agcaggcggc tgggccgctc cttgccgtct tccaccgaca gggcaggctg ggtcgggatc
     1501 tcgcggaaca tcagcaccag gaagacggcg cccgcctcct gggtgttgat ctggtagcgg
     1561 aagggctggg ccacgatgat cagccggccc tccgggtcca gcgccacgcc ggggcggatc
     1621 cagaccgagc ggtccgcggg gtcggcggcg tccacctcca ggcccgcgac gatgccccag
     1681 ccgtgcagcg ccagcgcgtg ggccgcttcg gcctggcggt ggtaatcgtg cgagtcgcgc
     1741 caggtctcgg cgtcgatcat gagacccagg aagggatcga cccgcttgag ttgcacgcgg
     1801 ggtacggcgt cgtccatgct ggctatctcc tcttcctcat gctgagggca cccgcgggca
     1861 ggtcgagctt gaccacgcgc aggacataag tggtttccac gggcttatcg gcttcgataa
     1921 tgccgcgcag ggtgtccata tcgagttcgt cgggccgggg cacggcgacg acgacggtga
     1981 aggtctgcgg gcggttctcg ccgatggacg tgttgaggcc gaggcgggca tcggggtcga
     2041 ggcggaagcc gttggtgcgc tccatgacca gcggcaggcc gccggtgtaa atgccgacgt
     2101 gctgcttgat gccggcgcgg gtgccgcgca tgcggtagag cgcggcggcg gcggcgatca
     2161 gggcgcggcg gcgcggcagc ggccactcct ccccttcgtg cagatcgacc cacatcgcca
     2221 gccagtcgag cagttcgacc ggggcggtca tggggtcgaa gtagtagggc aggttgtcga
     2281 ccatgaccga gatcgggtcg aacacatcct cgaagatgcg caggaagcgc ccggcgaact
     2341 cgtcggcggc gtagatggcc ggcagcaggc gcaggtagct cgacggctcc ggcgtcttgc
     2401 cgtcggcgaa gcccaacggc atcgggccgt ggccgttgct gctgatggtg cggggcgcgt
     2461 cgggcacggt ggccgccagc gcgctcaggt cttgcggtgg gctcatttga tggtcaccac
     2521 gtgattatag gacgccagca ggccgttggc cgggcaagta atcgtcccct cgacgggagt
     2581 gcgcgtgttg gtgatcatat cgaccacgta gaggcgcacg ctctcgacat actccacgcc
     2641 ctcgatgccg tgcaccaggg ccgtgacttc cgactggaac aggttgcgct cccacggcca
     2701 gccctcgccg tcgggcccgc cgtgcaccgg attgatgaag cgatagaggg cgcgctcgat
     2761 ctcgccgcgc aggccatcgc gcagggcgcg cttgcgggcg cccgcggtca cttccaccga
     2821 gacgaagcgg tattcgggcg ggtcgatctg caaagtggac gtgatcaggc ggcgctcgtt
     2881 gagataggtc atgacttcgc cgcgggcgcg gtcgctcagg gccagttcag gcggcgggat
     2941 ggccgagtcc gtatgggcgc tggccggcac gagcagcacg cggatgatcg cccggcccgc
     3001 ggccacctcg gtcgggctgg gggccaggca gcgggcgcgc aacacttcgg gcgtggcccg
     3061 tttggcgagg tactcgtagt cctcggcggt cacggcgcgc gagcgcgagc gcagcagttg
     3121 cggcccgcgc agcagggcca tgtcgatggt ctcggcatcc tccccgccga tggccgggcg
     3181 cgcgttggcg accgtggcga tgtaggggat ggacgacttg agcacggtga tcgtgttctt
     3241 gccgacgttg ccgcgggtgc cgccgccggt gcggtagccg gtgaagcgca gcagccggcc
     3301 ctcgaccggg tgggcgccgt actggatctc gcggcccccc gagtcgcgga tgagcgggcc
     3361 gaagcggatc acgccggtca ggctatcgag catgtagtgg gcgtcgccgg ggcgcgaggc
     3421 gccgaagtcc tcgacctcct gccagcccag gaaactgccg tcttcctgct cgacttccaa
     3481 aaactcgccg tcgtcgcggg ccagcacggg cgggtggctc aactgccact cctggccggg
     3541 caggccggtg gcccggccca gcacttcctg gccgatgaac tcgacctggc tggcgggcac
     3601 caggccgccc agcgccaccg tgttgacctc gcggatgcgc ggcgccgccg agtaggcggg
     3661 ctggttgggc cggggcgccg tggcccggca gcggatccag aaggccacgt ggccgtcgat
     3721 gtcgcgcggg cgggcgccga agggcacatg gaccaccacc tcgccggggc ggttcaggcc
     3781 gccagtggtg tcgctctcca ggtagagcgc gccccattcc tgcgcggggc cgtcccagta
     3841 ctcccaggcc agcggcgggt ccttggggtt gacgccgatg ccctcgatct ggctgtccac
     3901 gtccaggcgc aggatgtggc cgcgcagatc ttcgtagtag ccgaaataca tggcgtcgtt
     3961 gggctgcggc gggtcctgga agatgcccat gttgagcgcg ggcgtgcgca gcaccggcat
     4021 atagtcctgg taggtgtagc cgtcgcggtt gacgcgcagc cccgagagcg tgggcacata
     4081 gatcgtcaga tcctggtcgg tggagaaggt gatcgcctcc tgcgactcgg tgcgcacggt
     4141 cgccacctgg ctgccggcgg ggatagtgac ggcttcgggt tgcgggccgg tcaggtagaa
     4201 ggtgatgtcg gcgcgggcgg gccgcggcgg ctccagcttc acgcccagca tctccagcca
     4261 cttgatgtag ttgcgtagcg gcacccggtt gatgcgatag agcagaatgt ccgtcatgta
     4321 ggcgaacagc tcgatcagcg tcacgccggg atcggagacg ttgtgatcgg tccactccgg
     4381 gcagtagcgc gggatgagcc gcttggcgtc gttgaccaga tcctggaaac tgcggtcgtc
     4441 cagcttgggg acaggtagta ccatgctcag ttactccgac cggataaggt agaaaggata
     4501 gaccaggttg cgctcgtcgt tggtggcgcg gacgcgataa tgaatggtga tgatcagcag
     4561 gttgcgctcg tcgtcgtcgg tgtccacgtt gacttcgagg acttcgacgc gcggctccca
     4621 ccagccaatc gcctcttcga catagctttc gaccagcccg gcggtggtgg cgttgttggg
     4681 cgcgaatacc agttcatgga tgccgcagcc gaactgcggg cgcatgtgcc gctcgccctt
     4741 ggctgtgctc aagat
//
LOCUS       DASUYC010001027         4870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1560158, whole genome shotgun sequence.
ACCESSION   DASUYC010001027 DASUYC010000000
VERSION     DASUYC010001027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1560158"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(571..2502)
                     /locus_tag="VF276_16810"
     CDS             complement(571..2502)
                     /locus_tag="VF276_16810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015758205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6608565.1"
                     /translation="MGPGGPGGRRPGGPMGGPFGGMGMPVEKAKDFKGSARRLIAYLR
                     PHRAALGAVLLMAILSQVFSILGPKILGQATTKLFEGLIAKYQGVPGASIDFNYISQI
                     LMILAGLYVISSAFSYLQQYIMAGVAQRTVYDLRQDVDQKLTRLPLKFYDARTHGEIL
                     SRVTNDVDNIANTLQQSATQILTSVVSIIGIIIMMLTISPWLTLIALVVLPVSMVIAA
                     TVAKRSQRLFIAQQRALGELNGHVEEMYGGHKIVKAFGREDESVARFNAINTRLYDAG
                     WKAQFMSGLIMPLLGFVGNIGYVFISIIGGIFVTQGRIAIGDVQAFIQYSQQFTWPIT
                     QMASLANVVQLAVASAERVFELLDEPEESPDAVPAEVIADPQGAVRFDHVKFGYLPDT
                     PVLKNLNIDVHPGQTIAIVGPTGAGKTTLVNLLMRFYDVDSGSITVDGVDIRSMQRGN
                     LRNIFGMVLQDTWLFNGTIRDNIAYGRLGATEEEIVQAATAAHADHFIRTLPDGYDTI
                     LNEEASNISQGQRQLLTIARAILADPAILILDEATSSVDTRTEVLIQRAMETLMQGRT
                     SFVIAHRLSTIRDAGLILVMNHGDIIEQGTHAELLAAGGFYADLYNSQFTGATLDEAL
                     AQPQPHPALAFAPAGRAAS"
     gene            complement(2550..4280)
                     /locus_tag="VF276_16815"
     CDS             complement(2550..4280)
                     /locus_tag="VF276_16815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018754574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6608566.1"
                     /translation="MFSLSRFLRPFALMIAGVLALQFGQSMAQLYLPTLLSDIVDTGI
                     VKGDIDYIWRIGMFMLLIAGLGTICAILATLLAARVSTGLGRHLRTAVFAQVSRFSLH
                     EFDKLGTATLITRTTNDINQVQMVWQIIMVMMITAPIMMIGGVIMAVSKDATLSLIFV
                     VAVPILALTITLIALRAVPLFRAMQKKIDKLNLVLRENLTGIRVIRAFNRSEYEKERF
                     TAANADLTATAIKVNRTMALLFPILLLVMNFTTIAIIWFGGIRIDSGGMQVGDLIAFL
                     QYAMQIMWSLLMVSMLFIMVPRAQASSTRINEVLNMQPDITDPAQAQTPTGEHGLVEF
                     RDVTFSYPGAEQPALCDISFTARPGEVVAVIGGTGAGKSTLVNLIPRFYDIDSGHILV
                     DGVDVREMDQETLRAKIGFVPQQAVLFTGTVAENIRYGKEDATDDEVREATTTAQALD
                     FVTAMPEGFDSLIAQAGANLSGGQKQRLSIARALVRRPEVYIFDDSFSALDFKTDARL
                     RRALRRETTQATVIIVGQRVTTIMDADRILVLDEGRIAGQGTHKALLQTSEVYREIVA
                     SQLSEEELVA"
     gene            4681..>4870
                     /locus_tag="VF276_16820"
     CDS             4681..>4870
                     /locus_tag="VF276_16820"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608567.1"
                     /translation="MNAGAYGVVAALEQADGSGVAPADLRGLAARRPWLALALFVSLV
                     SLVGIPPLGASSASSASSA"
BASE COUNT          876 a         1453 c         1661 g          880 t
ORIGIN      
        1 agaaaccagt atatgagtcc agcattcagc ccatgtagta ggagggacac gacgtgaaac
       61 ggggttgggt gaaggccgaa agccagttgg ccgatccagt agaaagtctt gacgagtggg
      121 cgataaaacc accaggtatg cgggtcaaag aacgcaggaa cctcagcaag cgaggctaca
      181 tgtgtccaag ataagaggac gaaatcgtcc gcgagaaagt agctggtgag gttaaggcag
      241 aagggtagga gggcaagaag caacagaccg attgctattc ggtggggatg ggcgcgcagc
      301 acacctactg tcttgagccc ttgcttcacg ctggtgtggg gcgagcatac agtcatgcga
      361 tctcaaaacg gtagttgttt aaaccaagtg gatcaatgct tgtcgccatt atactatgaa
      421 atctcgacga tgaatacaca aaaaggcccg gacccaccag caaacgtggg tccgggcctt
      481 ttttatgggg cggcggaccg gtttgaaacc ggcccctaca ccgcaacggg gcgggccagt
      541 ttgaaaccgg cccctacacc gctggcgggg ttacgaggcg gcgcggcccg cgggggcgaa
      601 ggccagggcg ggatgcggct gcggctgtgc cagcgcttcg tccagggtgg cgccggtgaa
      661 ctggctgttg tagaggtcgg cgtagaagcc gcccgccgcc agcagttcgg cgtgggtgcc
      721 ctgctcgatg atgtcgccgt ggttcatcac caggatcagg ccggcgtcgc ggatggtcga
      781 gagccggtgg gcgatgacga agctggtgcg gccctgcatc agcgtctcca tggcgcgctg
      841 gatcagcacc tcggtgcgcg tgtccacgct gctggtcgcc tcgtccagga tcaggatggc
      901 cgggtcggcc aggatggcgc gggcgatggt cagcaactgc cgctggccct gcgagatgtt
      961 cgacgcttcc tcgttcagga tcgtgtcgta gccgtcgggc agggtgcgga tgaagtggtc
     1021 cgcatgggcc gcggtggccg cctgcacgat ctcctcctcg gtcgcgccca ggcgcccata
     1081 ggcgatgttg tcgcggatgg tgccgttgaa cagccaggtg tcctggagca ccatgccgaa
     1141 gatgttgcgc aggttgcccc gctgcatcga gcggatatcc acgccgtcca cggtgatgct
     1201 gccgctgtcc acgtcgtaga agcgcatcag caggttgacc agcgtggttt tgcccgcgcc
     1261 ggtcggcccg acgatggcga tggtctggcc gggatgcacg tcgatgttca ggttcttgag
     1321 caccggcgtg tcgggcaggt agccgaactt cacatgatcg aagcgcacgg cgccctgcgg
     1381 gtcggcgatg acttccgccg ggaccgcgtc ggggctttcc tccggctcgt ccagcaactc
     1441 gaagacgcgc tcggcggagg ccacggccag ttgcaccacg ttggcgaggc tcgccatctg
     1501 ggtgatcggc caggtgaact gctgcgaata ctggatgaac gcctgcacgt cgccgatggc
     1561 gatgcggccc tgggtgacga agatgccgcc gatgatgctg atgaacacat agccgatatt
     1621 gccgacgaag ccgagcaacg gcatgatcag gccggacatg aactgcgctt tccagccggc
     1681 atcgtagagg cgggtgttga tcgcgttgaa acgcgcgacc gactcgtcct cgcgcccaaa
     1741 cgccttgacg atcttgtgcc cgccgtacat ctcctcgaca tggccgttga gctcgccgag
     1801 ggcgcgctgc tgggcgatga acaggcgctg cgaccgcttc gccacggtcg cggcaatcac
     1861 catggacacc ggcaggacga ccagggcgat cagcgtcagc caggggctga tcgtcagcat
     1921 catgatgatg atgccgatga tggagaccac cgaggtcagg atctgcgtgg cgctctgctg
     1981 caacgtgttg gcgatgttgt ccacgtcgtt ggtcacgcgg ctcaggatct cgccgtgcgt
     2041 gcgggcgtcg tagaacttga gcggcaggcg cgtgagcttc tggtctacgt cctggcgtag
     2101 atcgtagacg gtgcgctggg ccaccccggc catgatgtac tgctgcaggt agctgaacgc
     2161 cgaactgatc acgtacagcc cggccaggat catgaggatc tggctgatat agttgaagtc
     2221 gatgctggcg ccgggcacgc cctggtactt ggcgatcagg ccctcaaaca gcttcgtggt
     2281 cgcctggccc aggatcttgg ggccgaggat gctgaacacc tggctgagga tcgccatgag
     2341 cagcacggcg cccagggcgg cgcggtgcgg gcgcagatag gcgatcaggc gccgggcgct
     2401 gcccttgaag tccttcgcct tttccaccgg catgcccatg ccgccgaaag ggccgcccat
     2461 cggcccgccg gggcggcggc cgccgggtcc ccccggtccc atgggccggc ctgcaggcgc
     2521 attgccgttg ccgttacgct gtgctgcggt catgctacca actcctcctc ggacaactgc
     2581 gaggccacga tttcgcggta gacctcgctg gtttgcaaca gtgctttgtg cgtgccctgg
     2641 ccggcgatgc ggccttcgtc cagcaccagg atgcggtcgg catccatgat ggtggtgacg
     2701 cgctggccga cgatgatgac cgtggcctgg gtggtctcgc gtcgcagcgc gcggcgcagg
     2761 cgggcgtcgg tcttgaagtc gagcgccgag aagctgtcgt cgaagatgta gacctcgggc
     2821 cggcgcacca gggcgcgggc gatggatagg cgctgcttct gcccgcccga caggttggcg
     2881 cccgcctggg cgatcagcga atcgaagcct tcgggcatcg cggtcacgaa gtcaagcgcc
     2941 tgggcagtgg tcgtggcctc gcgtacctcg tcgtcggtcg cgtcctcttt gccgtagcgg
     3001 atattctcgg ccacggtgcc ggtgaagagc acggcctgct ggggcacgaa gccgattttg
     3061 gcccgcagtg tctcctggtc catctcgcgc acgtccacgc cgtccaccag gatgtggccg
     3121 ctgtctatgt catagaagcg cgggatcagg ttgaccagcg tcgatttgcc cgcgccggtg
     3181 ccgccgatga cggcgacgac ttcgccgggc cgcgcggtga acgagatgtc gcagagggcg
     3241 ggctgctcgg cgccgggata gctgaacgtg acgtcgcgga actcgaccag gccatgctcg
     3301 ccggtcgggg tctgcgcctg ggccgggtcg gtaatatcgg gctgcatgtt cagcacctcg
     3361 ttgatgcgcg tcgaggacgc ctgggcgcgg ggcaccatga tgaacagcat cgacaccatg
     3421 agcagcgacc acatgatctg catggcgtac tgtaagaagg cgatcaggtc gcccacctgc
     3481 atgccgccgc tgtcgatgcg gatgccgccg aaccagatga tggcgatggt cgtgaagttc
     3541 atcaccagca gcaagatcgg gaagagcagg gccatggtgc ggttgacctt gatcgccgtg
     3601 gcggtcaggt cggcgttggc cgcggtgaag cgttccttct catactccga gcggttgaag
     3661 gcgcggatga cgcggatgcc ggtcaggttc tcgcgcagca ccaggttgag cttgtcgatc
     3721 tttttttgca tggcgcggaa cagcggcaca gcgcgcaggg caatcagcgt gatggtgagg
     3781 gccaggatcg gcaccgcgac gacgaagatc agcgacaagg tggcgtcttt ggagacggcc
     3841 atgatcacgc cgccgatcat catgatcggg gcggtgatca tcatgaccat gatgatctgc
     3901 cacaccatct ggacctggtt gatgtcgttg gtggtgcggg tgatcagcgt ggccgtgccc
     3961 agcttgtcga actcgtgcag cgagaagcgc gacacctggg cgaagaccgc tgtgcgcagg
     4021 tggcgtccca gcccggtgga gacccgcgcc gccaataacg tggcgagaat cgcgcagatg
     4081 gtccccagcc cggcgatcag cagcatgaac atgccgatgc gccagatgta atcgatgtcg
     4141 cccttgacga tgccggtatc gacgatgtcg gacagcaggg tcggcaaata gagctgggcc
     4201 atcgactggc cgaactggag tgccagcact ccggcgatca tgagggcaaa gggccgcaag
     4261 aaccgcgata aactgaacat acttccacac ctccgtacaa ccaaaaacca agtccctgtg
     4321 ggctacgctt cccgcgcgtg ccgaaagccg gcgcacacct ctcggcttag tcggctcagg
     4381 gctgaagcca atgatacaag gccaatatta attctatgta aaccgatagt tagctgggct
     4441 atctaatagt attagtatac cgccatgccg tcaatacgtc ataagtccca tattgccccc
     4501 gccagacggg tggtaatgtt gggccagacg gcaccagggg tccgccgctc cagcgcgccg
     4561 ggcgccgggg ccgccactta gcagccgatc ctctcgcgcc ggctatgccg gccagggcgt
     4621 cgtagcgaac acaagcgcag gcgccgcagg tgtgctatac tatctctggc atataccatc
     4681 atgaacgccg gggcctatgg ggtcgtggcg gcgctggagc aggcagacgg cagcggagtc
     4741 gcgccggccg atctgcgcgg gttggccgcc cggcgcccct ggctcgccct cgctctgttc
     4801 gtgagcctcg tctcgctggt cggcatccca ccgctggggg cttcttcggc aagctcggcg
     4861 tcttcggcgc
//
LOCUS       DASUYC010001028         3933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1791012, whole genome shotgun sequence.
ACCESSION   DASUYC010001028 DASUYC010000000
VERSION     DASUYC010001028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1791012"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..1170
                     /locus_tag="VF276_16825"
     CDS             229..1170
                     /locus_tag="VF276_16825"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6608568.1"
                     /translation="MLDADLDLGESWALVAERPAAPGAPPVLAGYMRAWWRHFGAEDS
                     QLMWMPADHLTCEIFFQFAAAAEEPPDAVFHTLFDAMLRATPAPPREPWVLSLVPPAA
                     GLDPALTALGFRLTSVFAYRPRDATPPTFPVPPGFQIRPATRQDTAALVDLYADLCAY
                     HVRNDPYADRIPPRLREEFAYVVRSVLAEPRRWVLLVACADTDPATPRAFSMASLDTE
                     DAHPAQITQLPPGTVGFIHDFMIAEALRGQGLGRALWAATLDALTARAPAAPPRGLHG
                     TWLIYRPTNPTGARFWPALGYTPLYHMWRRGGWTRDE"
     gene            complement(1311..1616)
                     /locus_tag="VF276_16830"
     CDS             complement(1311..1616)
                     /locus_tag="VF276_16830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608569.1"
                     /translation="MSDESGRPAAPDEDEYAQMLLLEDLESLAEEMDEIGVTDLAEVR
                     RWLSGDTTDPAAGVPGAADRAGLEAIARLMADLGVTSRADLATRIRTMQEQMDWGDE"
     gene            1874..3073
                     /locus_tag="VF276_16835"
     CDS             1874..3073
                     /locus_tag="VF276_16835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608570.1"
                     /translation="MKPQPRVLFSLAAIAGLGVSMLGFVGAGAQQFPEFAHPAFEVTW
                     NRTDSLVAEGRVPRSWYWGPHPNTPGIYETLTDAPDGSGRHLVQYFDKSRMEINNPAG
                     NANEKFFVTNGLLTVELIGGCIQTSTKSPCGEVRYPAEIPMSGDPGDQLAPTYAAFAN
                     VANSRFGDHPDPDRRGQQVTATIDRNGNVGDDPAKASVPLTKVAYYEASTKHNIPEAF
                     WNFLNQTGQVREQGQIVVAPLSSPWFYVTGLPISDAYWAHATIRGKDTEVLIQAFERR
                     VLTYTPSNTPAFQVEMGNIGQHYYDWRYGFFGQPGPGTGTPYNTPVNGGTPTYAPTGS
                     PGPVTTSVPTNTPGGAATATPESATATATTAAATATATEEPEDSATPTPTPTNTPRAS
                     TFDAPQQ"
     gene            complement(3149..>3933)
                     /locus_tag="VF276_16840"
     CDS             complement(3149..>3933)
                     /locus_tag="VF276_16840"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HEX6608571.1"
                     /translation="HNHAPGPGSGPLTYGSYLRVPELLALQSPLSLPTVHDEMLFIVV
                     QQAQELWFKQVLYELQTIIGLLDEGDLLDAARLIRRANAIFGVLGTEVDILATMPPLE
                     FGRFRGVLSTSSGFESEQFRELELASGLNDPTFFKVIDKYMDGAALRARWPRTLAEAF
                     RDVLTGLNPDPVAALVCVYGTPAAHPELFLLAETLSEYEVRFQEWRFHHVKLVERVIG
                     DRSPGTAGSAGSGYLGKTLTYRFFPELWEARNRLTAQGIGTR"
BASE COUNT          622 a         1475 c         1247 g          589 t
ORIGIN      
        1 gtgatcgaga ccgggcagat cgtcctcgaa gataccggcc cgaacttgct caagagcgag
       61 caggtgcgca aggcgtacct gggcgaggac taaccgctgc aaacccggct cctgcgccgc
      121 agcgacctcc ccgccgtcgc cgcgctcctg gccgatcagt accgccgctg gcacgcgctg
      181 gacccgcgcc tctcctgccc gccccaggtc gagcgggccg cccgccgcgt gctcgacgcc
      241 gatctagatc tgggcgaaag ctgggcgctg gtggccgaac gtccggcggc gcccggcgcc
      301 ccgcccgtcc tcgccgggta catgcgcgcc tggtggcgcc atttcggggc cgaagactcg
      361 cagttgatgt ggatgcccgc cgatcacctg acctgcgaga tcttcttcca gttcgccgcc
      421 gccgccgagg aaccgcccga cgccgtcttc cacacgctgt tcgacgccat gctgcgcgcc
      481 acacccgccc cgccgcgcga gccctgggtg ctgtcgctgg tgccgcccgc cgcgggcctc
      541 gacccggctc tgaccgccct cggcttccgc ctgacctcgg tcttcgccta ccggccccgc
      601 gacgccaccc cgcccacctt cccggtcccg cccggcttcc agatccgccc cgcgacgcgc
      661 caggacacgg cggccctggt cgatctgtac gccgatctct gcgcttacca tgtgcgcaac
      721 gacccttatg ccgaccgcat cccgccgcgc ctgcgcgagg aattcgccta cgtagtgcgc
      781 tcggtgctgg cggagccgcg ccgctgggtg ctgctggtgg cctgcgccga tacggacccc
      841 gccacaccgc gcgccttcag catggcctcg ctcgatacgg aggacgcgca cccggcccag
      901 atcacccagc ttccgccggg caccgtcggc ttcatccacg actttatgat cgccgaggcg
      961 ctgcgcgggc agggcctggg ccgtgccctg tgggccgcca cgctcgacgc gctcaccgcc
     1021 cgcgcgcccg ccgcgccccc gcgcggcctg cacggcacct ggctcatcta ccggcccacc
     1081 aaccccaccg gcgcccgctt ctggcccgcc ctcggctaca ccccgctcta ccacatgtgg
     1141 cgtcggggcg ggtggacgcg agacgagtag tcagtggtca gtggtcagta gtcagtggtc
     1201 agtggtcagt gatttcgcgc gctcgcgagt tccagccggc aggctatatg gtccagcgtg
     1261 gcactagact cgccactcgt cgtaactgac cactgaccac tgaccactga ctactcgtcg
     1321 ccccagtcca tctgctcttg catggtgcgg atgcgcgtgg cgaggtcggc gcgcgaggtg
     1381 acgcccaggt cggccatcag gcgggcgatg gcctccagcc cggcgcggtc cgcggcgccc
     1441 ggcacgcccg ccgccggatc ggtcgtgtcg ccgctcagcc agcggcgcac ctcggccagg
     1501 tcggtcacgc cgatctcgtc catctcctcg gccaggctct ccaggtcttc gagcagcagc
     1561 atctgcgcat attcgtcctc atcgggcgcg gcggggcgcc cggattcgtc gctcatcggc
     1621 ccatcctcct gcgccggaat cgagcaagaa gcctgccctc gcgcggcgag cgcggctgca
     1681 acccgccccc ggcccattcg ttacaccgat agacccacgg gtcacgagcg gaccggtctc
     1741 gcattgcgtg cctcttcacc ggcgctgctc gtgacctgta ggcatagttc gtgctgggca
     1801 aaggcgcctg ggccgtagca ctttgcccgg acgcctttga cccctaacgt accctgacag
     1861 gaggaattcg cctatgaaac cgcagcctcg tgttctattc agcctggcgg ccatcgccgg
     1921 cctgggtgtg tccatgttgg gcttcgtcgg ggccggcgcg caacagtttc ccgagttcgc
     1981 gcacccggct ttcgaggtta cctggaatcg caccgactcg ctggtcgccg agggccgcgt
     2041 gccgcgtagt tggtactggg gcccgcaccc gaacaccccc ggtatctacg aaacgctgac
     2101 cgatgccccc gacggctcgg gccggcacct ggtgcagtat ttcgacaaga gccgcatgga
     2161 gatcaataac cccgccggca acgccaacga gaagttcttc gtgaccaacg gcctgctgac
     2221 cgtcgaactg atcggcggct gcatccagac cagcaccaag tcgccctgcg gcgaagtgcg
     2281 ctacccggcg gagatcccga tgtcgggcga ccccggcgac cagctcgcgc ccacctatgc
     2341 cgcgttcgcc aacgtcgcca atagccgctt cggcgaccac cccgaccccg accgccgcgg
     2401 ccagcaagtc accgcaacca tcgaccgcaa cggcaatgtc ggcgacgatc cggcgaaagc
     2461 ctctgtgccg ctgaccaaag tcgcctacta cgaagccagc accaagcaca acatccccga
     2521 agccttctgg aacttcctga accagacggg ccaggtccgc gagcagggcc agatcgtggt
     2581 cgcgccgctc agcagcccct ggttctacgt caccggcctg cccatcagcg acgcctactg
     2641 ggcgcacgcc acgatccggg gcaaggacac cgaggtgctg atccaggcgt tcgagcgccg
     2701 cgtgctgacc tacacgccga gcaatacgcc cgccttccag gtggagatgg gcaacatcgg
     2761 ccagcactac tatgactggc gctatgggtt cttcggccag ccggggcccg gcacgggcac
     2821 gccgtacaac acgcccgtga acggcggcac gccgacctat gcgccgacag gcagccccgg
     2881 cccggtgacg accagcgttc ccaccaatac ccctggaggg gccgccacgg ccaccccaga
     2941 gtccgccacg gcgacggcca cgacggcagc cgccacggcc accgcgaccg aggagccgga
     3001 agactcggcc acacccacgc ccacgcccac gaacacgcca cgcgcctcga ccttcgacgc
     3061 gccccagcaa taggccgggg cgggcaggac aaacacggga gccgcgcgca ggcttgcgcg
     3121 cggctcctct ttgtgcgcgg ggtgccgctt agcgtgtgcc gatgccctgg gcggtgagcc
     3181 ggttgcgcgc ctcccagagt tcggggaaaa agcgataggt gagtgtcttg cccaggtagc
     3241 cgctgcccgc cgagccggct gtgcccggcg accggtcgcc gatgacccgc tcgaccagct
     3301 tgacgtggtg gaagcgccat tcctggaagc gcacctcgta ctcgctcaga gtctccgcca
     3361 gcaggaacag ttcggggtgg gccgcgggcg tcccgtagac gcagaccagg gcggccaccg
     3421 ggtcggggtt cagcccggtg agcacgtcgc ggaaagcctc ggccagggtg cgcggccagc
     3481 gggcgcgcag cgccgcgccg tccatatact tgtcgatcac cttaaagaag gtgggatcgt
     3541 tgaggcccga ggccagttcc agttcgcgga attgctcgga ctcgaagccg ctggacgtgg
     3601 acagcacgcc ccgaaagcgg ccaaactcca gcggcggcat cgtcgccagg atatctacct
     3661 cggtccccag cacgccgaag atcgcgttgg cacgcctgat gagccgcgcg gcgtccagca
     3721 ggtcaccctc gtccagcaga ccgatgatag tttgcagttc gtagagcacc tgcttgaacc
     3781 agagctcttg cgcctgctgc acgacgatga acaacatctc atcgtgcacg gtgggcaggc
     3841 tgagcggcga ttgcaaggcc agcaattcgg gcacgcgcag gtaggagccg taggtcagcg
     3901 ggccggaacc ggggccgggg gcgtggttgt gct
//
LOCUS       DASUYC010001029         2014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_2022369, whole genome shotgun sequence.
ACCESSION   DASUYC010001029 DASUYC010000000
VERSION     DASUYC010001029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2022369"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VF276_16845"
     CDS             complement(<1..797)
                     /locus_tag="VF276_16845"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEX6608572.1"
                     /translation="MDRYLEQQANTVEDVLAAHGIPARVAGGTLSPRLIHFLLELAPG
                     LRLAALTPALPDLAERLGVEAVRLAPDAEHGRPTLEVPRPDPVPVRLLPLARSVAEIA
                     PPCTATLGSDVGGSPLLLRLDAPEVGPTLVAGEASAGKSALLRSMALSLALHNGPDTL
                     RFLLIDLSGPARRGARSGTWSDMDRLPHLIGEPVRDLLEARLRLRWAARLVESRAELA
                     ADGRPIEGAHLLVLIDGLDALLAGRDAAEMEAAIKTIAAGRDLGVELV"
     gene            1331..>2014
                     /locus_tag="VF276_16850"
     CDS             1331..>2014
                     /locus_tag="VF276_16850"
                     /inference="COORDINATES: protein motif:HMM:NF024298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX6608573.1"
                     /translation="MRNLRWILAGVGLLILTALVGWAALQLTQPANVLAGHQGAVSAV
                     AFAPDGQTLASASLDDTIRLWRVADRRLLRILKAPENTGGVNAVAFAPDGQTLAAGYG
                     DGQVRLWRVADSALLRMFASHTEAVQGVAFTPDGRTLASASFDGTVRLWQVTNGTLLH
                     TIAVGLGKLKAVAIAPDGATLAVGGEAGRVSLWWVADGTPAAPLGDPGRGGSIPVENS
                     VAFAPDGTVV"
BASE COUNT          341 a          679 c          666 g          328 t
ORIGIN      
        1 accagttcca cgcccagatc gcggcccgcg gcgatggtct tgatcgccgc ttccatctcg
       61 gcggcgtcgc ggcccgccag cagggcgtcg agaccgtcga tcagcaccag caggtgcgca
      121 ccctcaatcg ggcggccatc cgcggccagt tccgcccggc tctcgaccag gcgcgcggcc
      181 cagcgcagcc gcaaccgcgc ctccagcagg tcgcgcaccg gctcgccgat caagtggggc
      241 aggcggtcca tatcgctcca ggtgccgctc cgcgctccgc gtcgcgccgg cccgctgagg
      301 tcgatcagca ggaagcgcag cgtatcgggg ccgttgtgca gcgccagcga cagggccatc
      361 gaacgcagca gcgccgattt ccccgcgctg gcttccccgg cgacgagcgt cggacccacc
      421 tccggcgcgt cgaggcgcaa gagcaagggc gacccgccca cgtcgctgcc cagggtggcc
      481 gtgcagggcg gcgcaatctc ggccacgctc cgcgctagcg gcaacaggcg cacgggcacc
      541 ggatcggggc gcggcacctc tagagtgggc cggccatgct cggcgtccgg agccagccgc
      601 actgcttcga cccctagccg ctcggccagg tcgggcaggg ccggggtcag cgctgccagc
      661 cgcaaaccgg gcgccagttc cagcaggaag tggatcaggc gcggcgacag cgtgccgccg
      721 gccacccgcg ccgggatgcc gtgcgccgcc agcacgtcct cgaccgtatt ggcctgctgt
      781 tcaagatatc ggtccatcgc gcatctccaa ccacaactta caactatctt tacggttctt
      841 cattcaactc gccggcctcg cgaagccggc acgcgcaaca acactccaac gttcagccac
      901 caggtcgcgg aggcaggggt gcctcagggg cgggcggggt ggcgcttctg tgcggtacgc
      961 cggtaaaggg gatgccaaat caaggtagaa cactagttct actcaccaat tataccgaac
     1021 aagcgtgcgg atgtcaatac cttttgtgcc tggatcagcc tgtgataagg ttgtaagaac
     1081 cgatgccaac aagctcttgc gtaccaggag agagcgtgat aaaatataca aaatgtgccc
     1141 gtgtccacca actttgtagc taaatttcgc cgccatccag aggcgcttcc ggctggggct
     1201 atgcgtaccg cccaactcac gattaccacc acgaactcca caccgcaata cctgatgaat
     1261 ctgacggtga cggggtcaaa tgccacccgg tcgcacagcc aaatcgtcag cctgaatcct
     1321 cagtaagccg atgcgcaatc tgcgatggat cctggctggt gtgggcctgc tcatcctgac
     1381 ggccctggta ggctgggccg cgctgcaact tacccagccc gctaacgtgc tggccggcca
     1441 tcagggggcg gtgagcgcgg tcgcctttgc ccccgatggg caaacgctgg cctcggcttc
     1501 cctggacgat acgatccgcc tgtggcgtgt ggctgaccgg cgcttgctgc ggatcctgaa
     1561 agcaccagag aatacggggg gggtgaacgc cgtggccttt gcgcccgatg ggcagaccct
     1621 cgccgccggc tatggcgatg ggcaggtgcg cttgtggcgg gtggccgaca gcgccctcct
     1681 gcggatgttc gccagccaca ccgaggccgt acagggcgtg gccttcactc ccgacgggcg
     1741 gaccctggcg tctgcctctt tcgacggcac cgtgcgcttg tggcaggtca ccaacgggac
     1801 ccttctgcat accattgccg tgggcttagg gaagctgaag gcggtggcta tcgccccgga
     1861 tggcgcaacg ctggctgttg ggggggaagc ggggcgcgtg agtctgtggt gggttgcgga
     1921 tggcaccccg gcggcgccct taggcgatcc ggggcggggc ggatcgatcc ctgtcgagaa
     1981 cagtgtcgcc ttcgccccgg atgggacggt tgtg
//
LOCUS       DASUYC010001030         2845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_405952,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001030 DASUYC010000000
VERSION     DASUYC010001030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405952"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /locus_tag="VF276_16855"
     CDS             complement(<1..1044)
                     /locus_tag="VF276_16855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608574.1"
                     /translation="MADASDQGDLAHGIDLARQGRKEAAYASLRRALFSDGDSALLRS
                     WLAAVAPTPGESIQHLERALELEPGNSQVQASLDAARRQVGAESAPPASPRIVPPAFP
                     SEPVLPPPAAAAPGAELGADPVAPPAPPPVEPVRPHSVGEVMGTAGISVRRPMLGGAE
                     PAAEGAARPWVPADSGTATSRPRRPPPDRPARNRMLPVLLGLIVVLLLIGVVSFMSLN
                     TGTLAGGVPGTVALAQTATATPNVAATAATTATAGAIAVATQTGVAQATIAVASTDYR
                     RAATQLMQDIRSQDGDIGNTIHQLDSGVLAPLDAAPMVAQYTRDQGAFAMRARNLDSP
                     VVYRQHQGQVMDAL"
     gene            1345..2754
                     /locus_tag="VF276_16860"
     CDS             1345..2754
                     /locus_tag="VF276_16860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HEX6608575.1"
                     /translation="MAQQGRLHGRGTEVAPARGTPPPRGGLGSWLPAGLGLRPWAALL
                     ILSLAVIGLGLAMLVANLYRMFIIWQTGTTFFYWATLQFIPHPWREILVAGVGIAGAV
                     LGYRGLARAVAAAQQRGRPVTSPAVLAPIRYSLPREGPRIVSIGGGTGQPTVLRGLKR
                     YTDHITAVVTVADDGGSSGRLRRDLGILPPGDFRNCLIALADVEPLMEELFQYRFGSD
                     AGELQGHPFGNLFLVAMTGVTGDFERAISECSRVLAVRGDIMPSTLANVTLNAELEDG
                     TIVSGESRLPEGKAAVRRAFLTPERPHGYPDAIAAILEADLIVLGPGSLYTSVIPNLL
                     VPDIVSAVRSSPAVKVYVCNVATQPGETDKFGVMEHVHALRKHVGPGVFDYVLVNADL
                     EPAGLIKPEWQVQAVTLPADTTYPGVEFVRAPVVNPHNPLRHDPERLAQALQNLYEEK
                     RQRDPAPTFSTNGRGSVGH"
BASE COUNT          415 a          958 c         1002 g          470 t
ORIGIN      
        1 gagcgcgtcc atcacctgtc cctggtgctg ccggtagacc accggcgagt cgaggttgcg
       61 ggcgcgcatg gcgaaggctc cctggtcgcg ggtgtattgc gcgaccatag gcgccgcatc
      121 gagcggggcc agcacaccgg agtcgagctg gtggatcgtg ttgccgatat cgccatcctg
      181 tgagcggata tcctgcatca gttgcgtggc cgcgcggcgg tagtcggtac ttgccaccgc
      241 aatcgtagcc tgggctacgc cggtctgggt ggcgacggcg atcgcccccg ccgtggcggt
      301 ggtggcggcg gtcgcggcga cgttcggggt ggcggtggcg gtctgggcca gggccacagt
      361 accgggcacg ccaccggcca gcgttccggt gttcagggac ataaagctca cgacgccgat
      421 cagcagcagc acgacaatca ggcccagcag aaccggcaac atccggttgc gggcgggccg
      481 atcgggcggt ggccgccggg ggcgcgaggt ggccgtgccg ctgtccgccg gcacccaggg
      541 ccgggcggcc ccctcggcgg ccggctcggc gccgcccagc atcgggcggc gcacgctgat
      601 gcctgcggtg cccatgacct cgccgacgga gtgcgggcgc acgggctcaa cggggggcgg
      661 tgcgggcggg gccaccgggt cggcgccgag ttccgcgccg ggcgccgcgg ccgcgggcgg
      721 cggcaaaacg ggttcgctgg gaaaggccgg cggcacgatc cgcggactcg ccggcggggc
      781 gctctccgcc cccacctggc gccgcgccgc atcgaggctc gcctggacct ggctattgcc
      841 cggttcgagt tccagggcgc gctccaaatg ctggatcgac tcgccgggtg tcggcgctac
      901 cgctgccaac caactgcgta acagcgccga gtcgccgtcg ctaaataagg cccggcgtaa
      961 actggcatag gccgcctctt tgcgaccctg gcgcgccagg tcgatcccgt gcgccaggtc
     1021 gccctggtcg cttgcgtctg ccatgcccac ccccatggtg tgaattcgtg cgcttgccga
     1081 tactactttc tttagaatat agtcaaaccg catgcttgtc aatcaggcgt ggaggcccgg
     1141 ttggcgctct gtaagcccga ccatcgccgc ttaaagcggc tggtaataac gcattgctag
     1201 agtggggata ccggcgcagg tgctttctga gtcggcatcc gccgtgcctg gcgcgtagcg
     1261 ctgtgagggg cgaggtgagt gtagtattta tggtataata gaacgccgtc tgaggcgacg
     1321 gcagctgttg aaggaggtag ctagttggca caacagggcc ggttgcatgg gcgcgggacc
     1381 gaggtcgccc cggcacgggg cacaccacct cccagagggg ggctggggtc atggctcccg
     1441 gcgggactgg ggttacgccc ctgggccgcc ttactgatcc tcagcctggc ggttatcggt
     1501 ctgggcctgg cgatgctggt cgccaacctc taccggatgt ttatcatctg gcagacgggg
     1561 accaccttct tttactgggc gaccctgcag ttcatcccgc atccgtggcg ggagatcctg
     1621 gtcgccgggg tcgggatcgc gggcgcggtc ctgggctatc gcgggctggc gcgcgccgtg
     1681 gcggcggccc agcagcgcgg gcgtccggtc acgagtccgg cggtgctggc gcccatccgc
     1741 tattcgctgc cgcgggaggg accgcgcatc gtctccatcg gcggcggcac cggccagccc
     1801 accgtgctgc gcggcctcaa gcgctatacc gaccacatca ccgcggtggt gaccgtcgcc
     1861 gacgacggcg gctcctcggg ccggttgcgc cgcgacctcg gcatcttgcc gccgggcgac
     1921 ttccgcaact gcttgatcgc actcgccgat gtggagccgc tcatggagga gctgttccag
     1981 tatcgcttcg gcagcgatgc gggcgaactc cagggccacc ccttcggcaa tctcttcctc
     2041 gtggcgatga ccggcgtgac cggcgatttt gaacgcgcca tcagcgaatg ctcgcgtgtg
     2101 ctcgcggtcc gcggcgacat catgccctcc accctcgcca acgtcaccct gaacgccgag
     2161 ctggaggacg ggacgattgt gtccggcgag tcgcgcctgc cggagggcaa agcggcggtg
     2221 cgccgggcct tcctgacgcc ggaacgccca cacggctacc cggacgccat cgccgccatc
     2281 ctggaagccg acctcatcgt gctggggccg gggtcgctct acaccagcgt gatccccaac
     2341 ctgctggtgc ccgatatcgt gagcgccgtg cggtcctcgc ccgcggtcaa ggtctacgtc
     2401 tgcaacgttg ccacgcagcc aggggagacc gataagttcg gggtaatgga gcatgtccac
     2461 gctttgcgca agcacgtggg accgggcgtc ttcgactatg tgctggtcaa tgccgacctg
     2521 gaacccgccg gcctgatcaa gccggagtgg caggtgcagg cggtcacgct gcccgccgac
     2581 accacctatc cgggcgtgga gttcgtgcgc gcgcccgtgg tcaatccgca caaccccttg
     2641 cgccacgacc cggagcgcct ggcccaggcc ttgcagaacc tctatgagga aaagcggcaa
     2701 cgtgacccgg cgcccacctt cagcaccaat gggcgcggtt ccgtgggaca ctaaccgtgc
     2761 gcggcttccg ggcggggcaa cacccggcgg gtccggcccc ggcggatttt tacggatctg
     2821 ccacgaacat tcaccgttgt ggcga
//
LOCUS       DASUYC010001031         2201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1791703, whole genome shotgun sequence.
ACCESSION   DASUYC010001031 DASUYC010000000
VERSION     DASUYC010001031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1791703"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VF276_16865"
     CDS             <1..354
                     /locus_tag="VF276_16865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6608576.1"
                     /translation="LIDLADEQLGLGLSVVLDAVFPLAGFRDEARAVAARHAARFRPV
                     YCYCSDEAVWRARVAARVQYVPGWTPVGWEEVERLRGFYEPWDPAAALFVEALDPPAA
                     NLARVVAYVQDGATA"
     gene            complement(344..1222)
                     /locus_tag="VF276_16870"
     CDS             complement(344..1222)
                     /locus_tag="VF276_16870"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6608577.1"
                     /translation="MAEPARDQWAEWLLRGRYGGDPAQAQTMVGALAPVREKVLANAA
                     LGPGATLLDVGCGDGLIAFGALPLVGLAGTVIFSDISQDLLDHCRGLAAESGVLDRCR
                     FLRAPADDLSAVALDSVDAVTTRSVLIYVAAKQAAFAEFYRVLKPGGRLSIFEPINRF
                     GGPATRLGGLDMAPIQEIADKIFAVYDRLQPFDSDPMLDFDERDLLTLAEQAGFREIH
                     LEYQATIKPMVPLAPWDLALRIPGNPRVPSLGEVMDTVLTPDEAARFVAYYRPFYENG
                     RGIARSAVAYLWALKQ"
     gene            complement(1245..>2201)
                     /locus_tag="VF276_16875"
     CDS             complement(1245..>2201)
                     /locus_tag="VF276_16875"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX6608578.1"
                     /translation="RTNNPWNLERTPGGSSGGAAAALAAGMTPLDIGSDFAGSIRIPA
                     HFCGLFGLKPTEHRVSMAGHIPDMPTEPRAHRIMWSIGPLARNVDDLALAFHLIAGAD
                     RGDPDVPPLLVTQPPPPPALADLRLAWAPTFPGLPVTAATRAALEHLAAALAENGARI
                     EEHLPEVSFEEQVRLRATLSKAIQAAFNDPENGPVPTLGEYFAALHRRDALIAAWEAF
                     FDDWDALICPVAMTNAFPHCPTDAPLLVDGVETKYWRIIGHCGIFNLTGHPVVVIPAD
                     QDADGLPIGVQVVGRRWADERLLAVAARLAEVAGPFRRPLGY"
BASE COUNT          322 a          748 c          821 g          310 t
ORIGIN      
        1 ctgatcgacc tcgcggacga gcaactcgga ctggggctaa gcgtggtgct cgacgccgtc
       61 tttccgctgg cggggtttcg cgacgaggcg cgggccgtgg ccgcccgcca cgccgcccgg
      121 ttccgcccgg tctactgcta ctgttcggac gaagcggtct ggcgggcgcg cgtcgcggcg
      181 cgggtccagt atgtgcccgg ctggactcct gtcggctggg aagaggtaga gcggctgcgc
      241 ggcttctacg agccctggga cccggcagcg gcgctctttg tcgaggcgct ggacccgccc
      301 gcggccaacc tggcgcgcgt ggtcgcctat gtgcaggatg gggctactgc ttgagggccc
      361 agaggtaggc gacggcggag cgggcgatgc cccggccgtt ctcgtaaaac gggcgatagt
      421 aggcgacgaa gcgggccgcc tcgtccgggg tcagcaccgt gtccatcact tcgcccaggc
      481 tgggcacgcg cgggttgccg gggatgcgga gcgccaggtc ccacggcgca agcggtacca
      541 tcggcttgat ggtcgcctgg tactccagat ggatctcgcg gaacccggct tgctcggcca
      601 gggtgagcag atcgcgctca tcaaagtcga gcatcgggtc gctgtcgaag ggctgcaacc
      661 gatcgtacac ggcgaagatc ttatcggcga tctcctggat gggggccatg tccagcccgc
      721 ccaggcgggt agcggggccg ccgaagcggt tgatcggctc gaagatcgac aggcgtccgc
      781 cgggcttgag cacgcggtag aattcggcga acgccgcttg cttggccgcc acgtagatca
      841 gcaccgagcg cgtggtcacg gcgtcgaccg aatccaaggc cacggcggac aggtcatcgg
      901 cgggcgcacg caggaagcgg cagcggtcca gcacgccgga ctcggcggcg aggccccggc
      961 agtggtcgag caggtcctgg gagatgtcgc tgaagatgac cgtcccggcc agcccgacca
     1021 gcggcagcgc gccgaaggcg atcaggccgt cgccgcagcc cacgtcgagc agggtggcgc
     1081 ccggccccag ggcggcgttc gccagcacct tctcgcgcac cggcgccagc gcgccgacca
     1141 tcgtctgcgc ctgggccggg tcgccgccat agcggccccg cagcaaccac tcggcccact
     1201 ggtcgcgcgc cggttcggcc atgcggctcc ctcctgctaa cgggttaata accaagcggg
     1261 cgccggaaag gtccggccac ctcagcgagg cgcgcggcca ccgccagcaa ccgctcgtcg
     1321 gcccagcggc gccccaccac ctgcacgccg atgggcaggc cgtccgcgtc ctgatcggcg
     1381 gggatgacca ccaccgggtg tccggtgaga ttgaagatgc cgcaatggcc gatgatgcgc
     1441 cagtatttcg tctccacgcc atccaccagc agcggggcgt ccgtggggca gtgcgggaag
     1501 gcgttagtca tggcaaccgg gcagatcagc gcgtcccagt cgtcgaagaa cgcctcccag
     1561 gcggcgatca gcgcgtcgcg ccggtggagc gcggcgaagt actcgcccag cgtgggcacc
     1621 ggtccgttct ccgggtcgtt gaaggcggct tgaatcgcct tggagagcgt ggcgcgcagc
     1681 cgcacctgct cctcgaagct cacctcgggc aggtgttcct cgatgcgcgc cccgttctcg
     1741 gccagcgcgg cggccaggtg ttccagggcg gcgcgagtgg ccgccgtgac cggcaggccg
     1801 gggaaggtgg gcgcccaggc caggcgcagg tcggccagag cgggcggggg cggcggctgg
     1861 gtcacgagca gcggcggcac atcgggatcg ccccggtcgg ccccggcgat caggtggaag
     1921 gccagcgcca ggtcgtccac gttgcgcgcc agcgggccaa tcgaccacat gatgcggtgg
     1981 gcgcgcggct cggtgggcat gtccgggata tgtccggcca ttgacacgcg atgctcggtc
     2041 ggcttgaggc cgaagaggcc acaaaagtgg gccgggatgc ggatcgaccc ggcgaagtcg
     2101 ctgccgatgt ccagcggcgt catccccgcg gccagcgccg ccgccgcccc gccgctgctg
     2161 ccgcccggcg tgcgctccag gttccagggg ttattggtgc g
//
LOCUS       DASUYC010001032         2114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_636573,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001032 DASUYC010000000
VERSION     DASUYC010001032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636573"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="VF276_16880"
     CDS             <1..876
                     /locus_tag="VF276_16880"
                     /inference="COORDINATES: protein motif:HMM:NF017000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase LanC family protein"
                     /protein_id="HEX6608579.1"
                     /translation="LDDSTLIGQARDLLRMLPADGAAEHDFLSGTAGAVAGLLVLAEA
                     LDDPALVARAASLGDALVEAADKSAAGYSWPARAIRPARNLTGLAHGAAGIGAALLEL
                     YAVTGDAAYRVAAEQAYAYERHWFDPDAGNWPDFRTTVGRNRRARAFAVAWCHGAGGI
                     ALTRLRAHALLGDARYEAEAAVALATTRRAADGALAAGGPDFSLCHGLTGHAEVVRSG
                     SAVLAVDDVVAAVAARGVAAARAAGGTWPCGTGGGETPGLLLGLAGIGLFYLRLHAPQ
                     IPMPLLPRPGLWSFD"
     gene            1189..1986
                     /locus_tag="VF276_16885"
     CDS             1189..1986
                     /locus_tag="VF276_16885"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6608580.1"
                     /translation="MGNRVQGKVAVVTGAAQGIGRGCAEMLAREGARVIAADVQAQRG
                     AAVVEAIRAAGGEAHFQPVDVTDEAQCAELMDAAVAAYGRIDVLVNNVGWYPRARLAD
                     TTTELWEQVLNVNLRSAFYCCKYAVPHLRAAGGGSIANIGSIHGLQAMANLVAYGAAK
                     GGLLNLTRTLAGALARDRIRVNYIIPGWVLTEGEIALQQGQGMSEDELRRAGATLPLG
                     RHQTPEDTAYAVVYLASDESSQVTGMVFHVDGGASTLLLSPDAPETL"
BASE COUNT          304 a          698 c          820 g          292 t
ORIGIN      
        1 ctggacgact ctaccctgat cgggcaggcg cgggacctgc tgcggatgtt gccggcggac
       61 ggggccgccg aacacgactt tttatcgggc acggcgggag ccgttgccgg gctgctggta
      121 ttggctgagg cgctggacga ccccgcgttg gtggcgcggg cggccagcct gggcgacgcg
      181 ctggtcgagg cggccgacaa gagcgcggcg ggctattcgt ggcccgcgcg ggccatccgc
      241 cccgcacgca acctgaccgg gctggcgcac ggcgcggcgg gcatcggcgc ggcgctgctg
      301 gagctgtatg cggtgacggg cgatgccgcc tatcgcgtgg ccgccgagca ggcatatgcc
      361 tacgagcggc actggttcga cccggacgcc ggaaactggc ccgatttccg cacgacggtg
      421 gggcgcaacc gccgggcgcg agccttcgcc gtcgcctggt gccacggcgc cgggggtatc
      481 gccctgaccc ggctgcgcgc ccatgcgctg ctgggcgatg cgcgctacga ggccgaggcc
      541 gccgtcgcgc tcgccaccac gcgccgcgcc gccgacggcg ccctggccgc gggcgggccc
      601 gatttctcgc tgtgccacgg cctgacgggc cacgcggagg tggtgcgatc gggatcggca
      661 gtgctcgccg tggacgatgt ggtggcggcg gtggcggcgc ggggagtcgc ggcggcgcgg
      721 gccgcgggcg gcacgtggcc ctgcggcacg ggcggcggcg aaacgcccgg cctgctgctg
      781 ggcctggcgg ggatcggcct cttttacctg cgcctgcacg cgccccagat ccccatgccg
      841 ctgttgccgc gccccggcct gtggtcgttc gattaggcgg cgcacggacc tgccggaggc
      901 acgggccggt ttcaaaccgg cccctacgtc agcacgaagc acccagtata atacgagagg
      961 cgcgggaatc gccggcgtcc ggccacgcaa atttcacgtc gtaggggccg gtttcaaacc
     1021 ggcctgccgc ccggcagaac aaccgtgggg accaggggcg gccacgctaa ttccgcgttg
     1081 taggggccgg tttcaaaccg gcctgtgctc gcagcggtgt aggggccggt ttgcaaccgg
     1141 cctgccgccc ggcaaaatca cgtggggaac aaagagaggg gacagcgaat gggcaaccgg
     1201 gtgcagggga aagtggccgt ggtgacgggc gcggcgcagg ggatcgggcg ggggtgcgcg
     1261 gagatgctgg cgcgcgaggg ggcgcgagtg atcgccgccg atgtgcaggc gcagcgcggg
     1321 gccgcggtgg tggaagcgat ccgtgcggcg ggcggcgagg cgcactttca gccggtggac
     1381 gtgacggacg aggcgcaatg cgcggagctc atggacgcgg cggtggcggc ctatgggcgg
     1441 atcgacgtgc tggtcaataa cgtgggctgg tatccgcgcg cccgcctggc cgacaccacg
     1501 accgaactgt gggagcaggt cctgaacgtc aacctgcgta gcgcgttcta ttgctgcaaa
     1561 tatgccgtgc cgcatttacg cgcggcgggc ggcggcagca tcgccaacat cggctcgatc
     1621 cacgggctgc aagcgatggc gaatctggtg gcctacggcg cggcgaaggg cgggctgctg
     1681 aacctgaccc gcacgctggc cggcgccctg gcccgcgacc gcatccgggt gaactacata
     1741 attcccggct gggtgctgac cgagggcgag atcgccttgc agcagggcca gggtatgagc
     1801 gaagacgaac tgcgccgcgc gggtgcgacc ctgccactgg gccgccacca gacgccggaa
     1861 gataccgcct acgccgtcgt ctacctggcc tccgatgagt cgtcgcaggt cacgggcatg
     1921 gtgtttcacg tggacggggg cgcgtcgact ctgctgctgt cgcccgacgc cccggagacc
     1981 ctctagcggt gccggtactc ccgccatccg cgcgcaagcc cgccagcagg cccagcagca
     2041 ggaagggcag cggcgaggtc aggtagccgt agccggcctc ggccttccag gcgatgatcg
     2101 tcaccgactc gaag
//
LOCUS       DASUYC010001033         4418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1328626, whole genome shotgun sequence.
ACCESSION   DASUYC010001033 DASUYC010000000
VERSION     DASUYC010001033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328626"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..984
                     /locus_tag="VF276_16890"
     CDS             142..984
                     /locus_tag="VF276_16890"
                     /inference="COORDINATES: protein motif:HMM:NF033520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEX6608581.1"
                     /translation="MDDIQIIAIFVIIDDLLQHLGHQDHVLAQASDAEVLTVAVVAAK
                     YLQNHVVAAKYFQNHQARSLSVLQRLGVLSGPLSLARFNRRLHRLADWLPSLVDLLGR
                     LFQQAECLIIDSLPLPVCRRIRAWRCRKVRGAAFCGYCAAKKEKFFGWRMHLVCTPAG
                     VPVRFAMLPAALHDLTPLHELLWELPAGTWVVGDKGYLSAADAASLLAATGVRLVARA
                     RDNMTPTSWEERQALEMYRQAIKTMNSQLERMGFERLYARTNTGFEIKAHASLLALTR
                     TNIN"
     gene            1074..2858
                     /locus_tag="VF276_16895"
     CDS             1074..2858
                     /locus_tag="VF276_16895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608582.1"
                     /translation="MRDKRRSEIAAAAVAAGIIVLAVGLRIALIAQGWPGPADSDEST
                     MGLMARHIAYSSAHPLLYYGQLIVGPAEAYAGAAVFQVASASVFALRLGIVLLFAPFL
                     ACMYALTRLLYGKAPALGVLGLLSLGSGEMLGAQVVAAGHAETPLYGAAIMLLATAIA
                     LAVAQRPGARHSWGYGAAYGGWGLLVGLAIWCDPLVVPFATTAGLLLLRTFRQGASKV
                     LAVWALLGLVLGSGPFLAGINGQMLAQPAVASTTVHALTDHPYFSRPLPSWLNLVGTV
                     MVSLPVATGANALWPVAPGIDFGWHPAQSYNVQSSWPGTQTLSFALSPATISHALWGL
                     AFIGLGVVAFRREYRRAHALAAPDRPSSWRRRRATTIYLARMALLGAAGLTLLVYLVS
                     PTPVLFPWAGKRYLVGLLVAFPAVLWPLVRRVPAGRWAPPVRAARHGLQAGGVAILAG
                     AFVAGVIQTWMAVPVAQAHWRAQDALVANLLELGATRIYGEYWTCNRLAFQTQEQIRC
                     SVINEEMQRGLDRYYPYCTEVDAALHPWYVFPRDSPQDLALAAQLAAQPAPYHLATFA
                     GYNAYQFDPPLLTEHPVATPATPAPAPP"
     gene            complement(3075..>4418)
                     /locus_tag="VF276_16900"
     CDS             complement(3075..>4418)
                     /locus_tag="VF276_16900"
                     /inference="COORDINATES: protein motif:HMM:NF015963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX6608583.1"
                     /translation="ADYSYPTLIVSPRNVDELIAVVKDHTRYPSPLRVAGHRHSMTPC
                     FATTGTQVLLQHFNDIRVDVDARTVTVGANVDMYQMRNALRRYGMQAEVSPEIGNATA
                     GSICCSGTKDSSLGPTGLGQLSSIVVEMKWVNARGEVEVINAARDPEKMHYARCSNGL
                     FGIIFEATFRIQKPVLLQYQYATFPVDRMPSRDVIFGGADGVLGFMTPYSNRIMAERR
                     TVIAPDAPISRLSRIKRKARDTYWENAATMSTTLWPYNRFYYLLDNLTAAGFLLLNSG
                     GGFRAHRDDSMVNFKFKRWHVADFTFWAMPISRLQEFMPAYYRLCKEFEQRTGFRMSL
                     LSEVYFLNRDNHSLLSYAPDEDIFTCDGINTNPHDPRWIEFNKRYNEFAAGYGGRPLF
                     TQTKYLDRAVVYRSLGNAWEQFLAERERQDPDGRFLNDYFRGLIRSEAREPAMAG"
BASE COUNT          715 a         1425 c         1437 g          841 t
ORIGIN      
        1 cgctgcaata ttcccctgtg gggaattccc aatctgaagt tcacagtagt tactgacgta
       61 ccttgattgc tagtttgctc ccatgaggaa ccacctctaa aatgccggtg cttgaaacca
      121 tcggcttaga ggaggccgca catggacgat atccagatta tagccatctt tgtcattatc
      181 gatgacctgc tgcagcacct cggccatcag gaccatgtac tcgcccaggc cagcgatgcc
      241 gaagtgctca ctgtcgcagt tgtggcggcc aagtaccttc aaaaccatgt tgtggcggcc
      301 aagtactttc aaaaccatca ggcgcggagc ctgagcgtgc tgcagcgttt gggcgtgctc
      361 tccggccccc tgagcctcgc gcgcttcaat cggcggctgc atcgcttggc cgactggttg
      421 ccctcgttgg tcgacctgct cggacggctg tttcagcagg ccgagtgcct gatcattgac
      481 agtttgcctc tgccagtatg ccgccgtatc cgcgcatggc gctgccgcaa agttcgcggc
      541 gccgcgttct gtggctactg tgccgccaag aaggaaaagt tctttggctg gcgcatgcat
      601 ttggtgtgca cacccgccgg tgtgccggtt cgtttcgcga tgctacccgc ggccttacac
      661 gacctgacgc cgctgcacga actgctgtgg gagctgcccg caggcacatg ggtcgtgggc
      721 gataaaggct acctcagcgc agcggatgcc gcgagcctct tggccgcaac gggcgtacga
      781 ctggtggcca gggcgcgcga caatatgaca cccaccagtt gggaggaacg gcaagcgttg
      841 gagatgtacc ggcaggccat aaaaaccatg aacagccaac tggaacgcat gggctttgag
      901 cgcttgtatg cgcgcaccaa caccggcttc gaaattaaag cgcatgcttc cctcctggct
      961 cttacccgta ccaacatcaa ctagcaatca aggttgacgt atgtttctgc tcggatcttg
     1021 ctcacgaacc ggttagggcg cgggacacca ggatcgcgcc aggagggggg agtatgcgag
     1081 acaagcgacg tagcgagatc gccgcagcgg ccgtcgcggc ggggatcatt gtactggccg
     1141 tcggcctgag gatcgcgctt attgcgcagg gctggccggg gccggccgac agcgacgaaa
     1201 gcacaatggg tctgatggcc cggcacatcg cctatagcag cgcgcacccg ctgctgtact
     1261 acggccagct catcgtcgga ccggctgaag cctatgccgg cgcggccgta tttcaggtgg
     1321 ctagtgcgtc ggtcttcgcc ctgcggctgg gcatcgtgct gctgttcgcg ccgttcctgg
     1381 cctgtatgta cgccctgacc cgcctgctgt atggcaaggc gccggcactg ggtgtgctgg
     1441 gcctgctcag cctcggctcg ggcgagatgc tgggcgcgca ggtcgtcgcc gccgggcacg
     1501 ccgaaacgcc gctctacggc gcggcgatca tgctgctggc gacggccatc gccctggccg
     1561 tggcccaacg acccggcgcc cgccatagct ggggctatgg tgccgcttat ggaggctggg
     1621 gcctgctggt cggtctcgcc atctggtgtg atccgctggt cgtgcccttc gcgacgacgg
     1681 cgggattact gctgctgcgc acgtttcggc agggcgcgtc caaagtgctc gccgtctggg
     1741 cgctgctcgg gttggtgctg ggcagcgggc cgttccttgc cggcataaac ggccagatgc
     1801 tcgcgcagcc ggccgtcgcg agcacaactg tacacgccct caccgaccat ccgtacttta
     1861 gccgccccct gccctcctgg ctcaacctcg tcggcacggt gatggtcagc ctcccggtcg
     1921 ccacgggggc caatgccctc tggccggtgg cgccgggcat tgatttcggc tggcatccag
     1981 cccaatcgta caatgtacaa agctcctggc cgggcaccca gacgctatcc ttcgcgctct
     2041 cgcccgccac gatcagccac gccctctggg gtctggcctt tatcggcctg ggggtggtcg
     2101 ccttccggcg cgaatatcgg cgcgcccatg ccctcgccgc gcccgaccgt ccgtcatcct
     2161 ggcggcgtcg ccgggcgacc acgatctacc ttgcgcgcat ggcgctgctg ggcgcggccg
     2221 gactgaccct gctggtgtac ctggtcagcc ctactccggt gctcttccca tgggctggga
     2281 agcgctacct ggtgggcctg ctggtcgctt tcccggcggt gctctggccg ctggtacggc
     2341 gtgtcccggc cgggcgttgg gcgccgccgg tccgtgctgc gcggcacggc ctgcaagcgg
     2401 gtggcgtggc gatcctggcc ggcgccttcg tcgccggagt catccagacc tggatggccg
     2461 tgccggtcgc ccaggcgcac tggcgcgcgc aggacgccct ggtggcgaat ctgctcgagc
     2521 tcggtgcgac gcgcatctac ggggagtact ggacctgcaa tcgcctggcc ttccagactc
     2581 aggagcagat tcgctgtagc gtaatcaacg aggagatgca gcgagggctg gaccgctact
     2641 acccgtattg caccgaggtg gacgccgcac tgcacccctg gtacgtcttc ccgcgtgact
     2701 ccccgcagga cctggcgctg gccgcgcaac tggccgcgca gccggcgccg taccacctgg
     2761 cgacctttgc cggctacaat gcctaccaat tcgacccgcc gctccttacc gagcacccgg
     2821 tagccacccc agcgactccg gccccggcgc cgccgtgaat cggctccggc gctgccgaag
     2881 ttttatcgta ttcgaagaat aatggttctt ccgcgaagtt gagaccactg cttttcgtgc
     2941 tcctgggcag tccaagacac acccgcggaa agttccagca caacatgcac gtcgtgccac
     3001 ctgcgcgcat caacttcgcg gaagaacgga ccggctattg attttacata atattcaggt
     3061 cgcgaacacc gaccttatcc ggccatcgcc ggttcgcgcg cctcgctgcg gatcagcccg
     3121 cggaagtagt cgttgaggaa gcgaccgtcg gggtcctggc gctcgcgttc ggccaggaac
     3181 tgctcccagg cgttgcccag gctgcggtag accacggcgc ggtccaggta cttcgtctgc
     3241 gtgaacagcg gccgcccgcc atagccggcg gcgaactcgt tgtagcgctt gttgaactcg
     3301 atccagcgcg ggtcgtgcgg gttggtgttg atgccgtcgc aggtgaagat gtcctcgtcg
     3361 ggcgcgtagg agagcagcga atgattatcg cggttgagga agtagacttc cgacagcagc
     3421 gacatgcgga acccggtgcg ctgctcgaac tccttgcaca ggcggtaata ggcgggcatg
     3481 aactcttgca ggcggctgat gggcatggcc cagaaggtga aatcggcgac gtgccagcgc
     3541 ttgaacttga agttgaccat cgagtcgtcg cggtgcgccc ggaacccgcc gccgctgttc
     3601 aggagcagga acccggcggc cgtcaggtta tccagcaggt agtagaaccg gttgtagggc
     3661 cagagcgtgg tgctcatggt cgcggcgttc tcccagtagg tgtcgcgggc cttgcgcttg
     3721 atgcgcgaga gccgggagat cggcgcgtcg ggcgcgatga ccgtgcggcg ctcggccatg
     3781 atccggttgg aatagggcgt catgaagccg agcacgccgt ccgccccgcc gaagatcaca
     3841 tcgcggctgg gcatgcgatc gacggggaac gtggcgtact ggtattgcag cagcaccggc
     3901 ttctggatgc ggaaggtggc ctcgaaaatg atcccgaaca gcccgttgct gcaacgcgcg
     3961 tagtgcattt tctccgggtc gcgcgcggcg ttgatgacct cgacctcgcc gcgggcgttg
     4021 acccacttca tctcgaccac gatggagctg agttgcccca gcccggtggg gccgagcgac
     4081 gagtctttgg tgcccgagca gcagatcgag ccggcggtcg cgttgccgat ctccggcgac
     4141 acctcggcct gcatcccgta ccggcgcagg gcgttgcgca tctggtacat atcgacgttg
     4201 gcgccgaccg tcaccgtccg ggcgtccaca tccacgcgga tgtcgttgaa gtgttgcagc
     4261 agcacctggg tgccggtggt cgcaaagcac ggggtcatcg aatgccggtg ccccgccacc
     4321 cgcagcggcg agggataccg ggtgtggtcc ttgaccaccg cgatcaattc atccacattc
     4381 ctggggctaa cgatcagcgt ggggtagctg tagtccgc
//
LOCUS       DASUYC010001034         2159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_942773,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001034 DASUYC010000000
VERSION     DASUYC010001034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942773"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..901)
                     /locus_tag="VF276_16905"
     CDS             complement(83..901)
                     /locus_tag="VF276_16905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010493965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HEX6608584.1"
                     /translation="MDILRHTAHRSEPLPAGPWIMAQTWHNLLFAHWPIALDALRPLI
                     PANLQIDTFEGQAWVGIVAFRLSGIRLRGFPEVGWVSHFPEINVRTYVTLDGKPGVLF
                     LSLDADNRAAIALAKPWFRLAYQAARIDHRPAVDGGYAFTARRTERTAPPATFSATYR
                     TTAPAFVAAPGTLAHWLTERYCYYSPGPRGQIYRCDIHHPTWPLQPAEAEITENTMAL
                     AHGLHLPQTAPLLHYAHYMQAAIWPLRRVCPPAAGMDPCRSARRLPQTEEIAAH"
     gene            complement(1083..1967)
                     /locus_tag="VF276_16910"
     CDS             complement(1083..1967)
                     /locus_tag="VF276_16910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608585.1"
                     /translation="MTNDQLAGQPQGSAGALRNSVAGTDRIFPEIRAIAAFIVLVLII
                     ASAILYLMPDRTAEFFAWTIKPNMTPLLMGAGYLSGAWFFVGALRAQGWHEITRGFFP
                     ITAFTWFMGLATLLHWDRFNHTHVTFYAWAILYVVTPFLVPALWFRNRVTDPDTLGAG
                     DVAVPERLRLAATVVGAGMLVIALFLFILPDVAISIWPWTITPLTARVVAGWFALPGV
                     VGLVLGREPRWSAWRILIQSQLIALALILAAVVRAWGDFKTANPLTWVFIAGITGILA
                     AALALYLSMETRRRTRAA"
BASE COUNT          403 a          640 c          780 g          336 t
ORIGIN      
        1 ggtcagcacc ccggtcggat acatccgggc gcgggcgaca agttgcttat tccacatact
       61 ggaccttcgc tccttcttat cttcagtggg ccgcgatctc ttcggtctga ggcaaccggc
      121 gggcggatcg gcaggggtcc atcccggcgg cgggcggaca gacgcggcgc agcggccaga
      181 tggcggcttg catgtagtgg gcgtagtgca gcagtggcgc ggtctggggt aggtggagac
      241 cgtgggccag agccatcgtg ttctccgtga tttcggcctc ggcgggctgc agcggccacg
      301 tggggtggtg gatgtcgcag cgatagatct gcccgcgcgg gccggggctg tagtagcaat
      361 agcgctcggt cagccagtgg gccagggtgc cgggtgcggc aacgaaagcc ggcgccgtgg
      421 tgcggtacgt cgcgctgaaa gtggcgggcg gcgcggtgcg ttcggtgcgc cgggccgtga
      481 aggcgtagcc gccgtccacc gcgggccggt gatcgatacg ggcggcctgg taagccaggc
      541 gaaaccacgg ctttgccagc gcaatcgccg cgcggttatc ggcgtccagg ctgaggaaca
      601 gcacgcccgg tttgccgtcc agcgtgacgt aagtgcgcac gttgatctcc gggaagtgcg
      661 atacccaccc gacctcgggg aacccccgca gccggatgcc gctgaggcgg aaggcgacga
      721 tgccgaccca cgcctggccc tcgaacgtgt cgatctggag gttcgccggg atcaggggcc
      781 gcagcgcgtc gagggcgatg ggccagtggg cgaacaagag attgtgccag gtttgcgcca
      841 taatccacgg tccggcgggc aacggctcgg atcggtgggc agtgtggcga agaatatcca
      901 ttccctatgc tcctgctgta actgatgcca acgccggctg aaatcgggcc gccacaaggc
      961 gcgaccgctc ctgtcaggac aagaagccga agtaccgatc tgtgcaatcg tgagtctgct
     1021 gggtagcggg gccggctcaa caccggctcc tacaagtaag cgtacgggag ttggtgaacc
     1081 gtctaggcgg cgcgggtgcg ccggcgggtt tccatgctca gatagagggc cagcgcagca
     1141 gcgaggatgc ccgtgatgcc ggcgatgaac acccaggtga gcgggttggc cgtcttgaaa
     1201 tcgccccagg cgcgcacgac ggcggcgagg atgagggcca gagcgatgag ttgcgactgg
     1261 atcaagatgc gccaggcgct ccagcgcggt tcgcggccca gcaccaggcc gaccacgccc
     1321 ggcagcgcga accacccggc caccacccgc gccgtcagcg gggtgatggt ccacggccag
     1381 atgctgatgg cgacatccgg taggataaac aggaacagcg cgatgaccag catgccggcg
     1441 cccaccacgg tggccgccag ccggaggcgc tccggcaccg cgacatcgcc cgcgccgagc
     1501 gtgtccggat cggtgacgcg gttgcgaaac cagagcgccg ggacgaggaa cggcgtgacc
     1561 acgtaaagga tcgcccaggc atagaaggtg acgtgggtgt gattaaaccg atcccagtgc
     1621 aacagggtag cgaggcccat gaaccaggta aacgcggtga tggggaagaa gccgcgcgtg
     1681 atctcgtgcc agccctgcgc gcgcagggcg ccgacgaaga accaggcgcc ggaaagatag
     1741 cccgcgccca tcagcagcgg cgtcatattc ggcttgatgg tccaggcgaa gaactcggcg
     1801 gtacgatcgg gcatgagata gaggatggcg gaggcgatga taaggacaag gacaataaac
     1861 gcggcaatcg cgcggatttc cgggaagatg cgatcggtgc cggcgaccga gttgcgcagc
     1921 gcgcccgcgc taccctgggg ttgcccggcc aactgatcgt tggtcatagt gcagtctcct
     1981 ctgtgtgctg actatcggtg tgccaacgct tattcatttg ccccacgaat taagaatagc
     2041 aaattcttgt gtgcgacaga tccgggattc cacggtattt gggccagact ccggtacggt
     2101 attgcggccc ggcgctgggc cggcatgtgg gaggtgcgcc ccgaggaacg catctccca
//
LOCUS       DASUYC010001035         5803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_447252,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001035 DASUYC010000000
VERSION     DASUYC010001035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5803
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447252"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="VF276_16915"
     CDS             complement(<1..770)
                     /locus_tag="VF276_16915"
                     /inference="COORDINATES: protein motif:HMM:NF014919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HEX6608586.1"
                     /translation="MADNLSPQIIDSIVHGYNGDPFSVLGPHAVTRDGQPAMAIRAFL
                     PWATAIQVARDDGTIHDMACIHPDGFFEAVIPAAGPFGYTLRATDPVGHVVDLIDPYS
                     FGPLLTDFDLYLIGEGTHHRTYEKLGAHIREVGGVRGVEFAVWAPNALRVSVVGNFNN
                     WDGRVYPMRFHPAQGIWEIFLPGLGEGEAYKYEIKSRFNDYLVEKADPYGFYSELRPR
                     TASVVADINRYTWGDTEWMSNRAKNNGQDSPIAIYECHL"
     gene            1021..4842
                     /locus_tag="VF276_16920"
     CDS             1021..4842
                     /locus_tag="VF276_16920"
                     /EC_number="6.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF009140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase subunit H"
                     /protein_id="HEX6608587.1"
                     /translation="MRFVVFTLEETYQQALTRGAALAAARSGIPCEVACYTVSRLARP
                     EVRAAAAADLARADAVLASMLFFDEHVQPLAALLDPLQKARPDLPVVILNSAPELMKR
                     TRLGGLRMDAGLAARLMGHAAARGPRKAPQADRPESDLARRLLGLSRSLPRLLRLVPG
                     ASSDLGRYLLLMQYWVHGTPENLAGLLLTLAAHYGPPAARKAAGSLAIAPPQVMPAAA
                     IFHPDAPAPFTSVAAYRRWRAERGLPGPGAGGTVGLVSLRSSILTGNTAHVAALVRAL
                     DAEGLEPVPTYAGGLDMRPATRRFFAPGPAPEGTDTAAVDVLINLSGFALVGGMAHND
                     APLAQAELARLDRPLLTGVPLNFQSLADWRASRTGVTPVQVAMQLAIPELEGAVEPVV
                     YGGIAEGGQPDFVAEPGNVARLAARAGRWARLARTPAAERKVAIVLFNFPPNGGAVGS
                     AAYLAVFPSLYRVLQELQAAGYAVDLPPDAETLRRLVVEGNAMEFGTPANVAAALATA
                     DYRRLCPWHDEIAAGGWGPPPGTLNSDGERILILGREFGSVFVGVQPPFGYEGDPMKL
                     LMSQGITPHHGFAGFYAWLRGIWGADAILHLGTHGALEFMPGKQAGLSADCWPSRLIG
                     ETPHLYVYSGNNPSEGSIARRRGLATLISYLSPPLEQAGLYRELLDLKASLRAVDGAV
                     PDAATLEAIGRQAGALHLPWPATAATDPAPALAGLRAALLDLESRLIPVGLHEIGRPP
                     AADELLDLLVQVSMYPRPEHDLPALAALIAGARGLTLPDDGVDAPPDGVPPAQLAALE
                     QVRALARAAIARLLDAGPEAAAEVLTREGIAAPATLPWLRWAAALAADLRRDAEPAAI
                     AAALDARYVPPSPGADVVRNPAVAPTGRNIYALDPYGVPTGMAWETGKRAAEALLARY
                     RREQGAWPRAIALVLWGTDNLKTAGEGIAQALWLLGVRPHRDALGRVADVRLVPLAEL
                     GRPRMDVVLTCSGIFRDLLPNQLTLLDRAVRLAAAADEPPEQNFVRRNALAATETGLP
                     FAEAATRVFSNAPGAYGANVNFQVETGAWDDDAALAQTFLARKSFAYGLGGEGVAARD
                     LLESALRPVDLAFQNIDSIETGISDIDHYFEYLGGVSKAVEILGGTAPAGFVLDVQGV
                     GGRLRSLGEMVAVESRTKLLNPRWSEGMLRFGYEGVREIGVRVANTLGWSATTRAVPG
                     WVYDGLAETYVLDAARREQMANLNPNAYQALVGRLLEAEGRGFWTPAPEVSAELRRVY
                     GEAEDLVEQVR"
     gene            4843..5448
                     /locus_tag="VF276_16925"
     CDS             4843..5448
                     /locus_tag="VF276_16925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608588.1"
                     /translation="MPSHPPTHTHMLAGSLLLCAMLPLLIACGAAAPAAPATVPPAAS
                     PTAALPTAPPTAAPATVPATEPPTVPETVAPTLAATTPPTTAPPTAIPATASPSGTPA
                     LARVITNADGGGQFTFAVGQTFSLQLSARRWSQPVVDPQILRALPVPTPAAPDASRWD
                     YQAVAPGRTDLTTEGACPPAPPGGVTCMSIELYKITIIVTP"
     gene            5596..5673
                     /locus_tag="VF276_16930"
     tRNA            5596..5673
                     /locus_tag="VF276_16930"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5631..5633,aa:Pro,seq:cgg)
BASE COUNT          807 a         2098 c         2028 g          870 t
ORIGIN      
        1 aggtggcact cgtagatggc gatgggtgag tcctggccgt tgttcttggc gcggttgctc
       61 atccactctg tgtcgcccca ggtatagcgg ttgatgtcgg cgacgaccga agccgtgcgc
      121 gggcgcagtt cgctatagaa gccgtaggga tcggccttct ccaccagata gtcgttgaag
      181 cgcgacttga tctcgtactt gtacgcctcg ccctcgccca gaccgggcaa gaagatctcc
      241 cagatgccct gggccgggtg gaagcgcatc gggtagacgc gcccgtccca gttgttgaag
      301 ttgccgacca cgctcacgcg caaggcgttg ggtgcccaga cggcgaactc cacgccgcgc
      361 accccgccga cctcgcggat gtgcgcgccg agtttctcat aggtgcggtg gtgcgtgcct
      421 tcgccgatca ggtagagatc gaaatcggtg agcagtggcc cgaacgagta cggatcgatc
      481 agatcgacca cgtggccgac gggatcggta gcgcgcaggg tgtagccgaa aggaccggcg
      541 gcggggatga cggcttcaaa aaacccatcc gggtggatgc aggccatatc gtggatcgtg
      601 ccgtcgtcgc gcgcgacctg tatggccgtg gcccagggca ggaacgcccg gatcgccatg
      661 gccggttgcc catccctggt tacggcgtgc ggtcccagga cgctgaacgg gtcgccgtta
      721 tacccgtgca cgatagaatc gatgatttgc ggggaaaggt tgtctgccac tgcaagctcc
      781 ttggcggaat aagtcaagcg gtttctcgcc ccctatgccg caactggcgt gccatgggaa
      841 gcggcggcat ggctgcgttc cccggcgggg cggttgcgcg cggcgggcat gtggggcggt
      901 tatggcttga gcgcgcgcgt cgccaggtgc gtgggcatgt agcgtgccag cggcgcgccg
      961 gggcagcgat cctcgtagaa gccggctact cagaagggcg ggagtcggct ataatgcact
     1021 atgcgcttcg tggtcttcac cctggaagaa acctatcaac aagccttaac ccgcggggcc
     1081 gcgctggcgg cagcgcgcag cggcatcccc tgcgaggtcg cctgctatac cgtcagccgg
     1141 ctcgcgcggc ccgaggtgcg cgcggcggcc gccgccgacc tggcgcgggc cgatgccgtg
     1201 ctcgccagca tgctcttttt cgacgagcat gtgcagcccc tcgccgcgtt gctcgacccg
     1261 ctgcaaaaag cgcgccccga cctgccggtg gtcatcctca acagcgcgcc ggagttgatg
     1321 aagcgcacgc gcctgggtgg gctgcgcatg gacgcgggcc tggcggcgcg cctcatgggc
     1381 cacgccgccg cgcgcgggcc gcgcaaagcg ccgcaggcgg atcgccccga gtccgacctg
     1441 gcccggcgct tgctggggct gtcgcggtcg ttgccgcgcc tgctgcggct ggtgcccggc
     1501 gcgtcgagcg acctggggcg ctacctgctg ttgatgcaat actgggtcca cggcacgccg
     1561 gagaacctgg ccgggctgct gctgaccctg gccgcgcatt acgggccgcc cgccgcgcgc
     1621 aaagccgcgg ggagcctcgc catcgcgccg ccacaggtga tgcccgccgc cgcgatcttc
     1681 caccccgacg cgccggcgcc gttcaccagc gtggccgcct accggcgctg gcgggccgag
     1741 cgcggcctgc ccggtcccgg cgcgggcggc acggtcggcc tcgtttcgct gcgctcgtcc
     1801 atcctgaccg ggaacacggc ccacgtcgcc gcgctggtcc gtgcgctcga cgccgagggc
     1861 ctggagccgg tacccaccta cgcgggcggc ctcgacatgc gcccggcgac gcgccgcttc
     1921 ttcgcgcccg gcccggcacc cgaggggaca gacaccgccg ccgtcgatgt gctgatcaac
     1981 ctgtcgggct tcgcactcgt gggcggcatg gcccacaacg acgccccgct ggcccaggcc
     2041 gaactggccc gcctggatcg cccgctgctg acgggtgtgc cgctcaactt ccagagcctg
     2101 gccgactggc gtgccagccg gacgggcgtc acgccggtgc aggtggcgat gcaactggcg
     2161 atcccggagc tggaaggcgc ggtcgagccg gtggtctacg gcggcatcgc cgagggcggg
     2221 cagcccgatt tcgtggccga gccgggcaat gttgcccggc tagcggcgcg ggcggggcgc
     2281 tgggcacggc tggcgcgcac cccggcggcg gagcgcaaag tcgccattgt cctgttcaac
     2341 ttccccccca acggcggcgc cgtgggcagc gcggcctatc tggccgtgtt ccccagcctc
     2401 taccgcgttc tccaggagtt gcaggccgcg ggctatgccg tcgatctgcc gcccgacgcc
     2461 gagacgttgc gccgcctggt tgtggaaggc aacgccatgg agttcggcac gcccgccaac
     2521 gtggccgccg cgctggcaac ggcggactac cggcggctct gcccctggca tgacgagatc
     2581 gccgcgggcg gctgggggcc gccgcccggc acgctgaaca gcgacggcga gcgcatcctc
     2641 atcctgggcc gcgagttcgg ctcggtcttt gtgggcgtgc agccgccgtt cggctacgag
     2701 ggcgacccga tgaagctgct catgtcccag ggcatcacgc cgcaccacgg tttcgcgggt
     2761 ttctacgcct ggttgcgcgg catctggggc gccgacgcca tcctgcacct gggcacgcac
     2821 ggcgcgctgg agttcatgcc gggcaagcag gcgggcctga gcgccgattg ctggccctct
     2881 cgcctgatcg gcgagacgcc gcacctgtac gtctacagcg gcaacaaccc gtcggagggc
     2941 agcatcgccc ggcggcgggg gctggcgaca ctcatcagct acctgtcgcc gccgctggag
     3001 caggccgggc tgtaccgcga actcctggac ctgaaggcga gcctgcgcgc cgtggacggc
     3061 gccgtgcccg acgcggccac cctggaggcc atcgggcggc aggcgggggc gctgcacctg
     3121 ccctggcccg ccacggcggc cacggacccg gccccggccc tcgccgggct gcgggcggcg
     3181 ctgctggatc tggagagccg cctgatcccc gtgggcctgc acgagatcgg gcgcccgcct
     3241 gctgcggacg aactgctcga cctgctggtg caggtgagca tgtacccacg cccggaacac
     3301 gacctgcccg ccctggccgc cctcatcgcc ggggcgcggg gcctgaccct gcccgacgac
     3361 ggcgtggacg cgccgccgga cggtgtgccc ccggcgcaac tggccgcgct ggagcaggtg
     3421 cgcgcgctgg cgcgggccgc tatcgcccgg ctgctggatg cggggccgga ggccgccgcc
     3481 gaagtcctga cgcgcgaagg gatcgcggcc cctgcaacgt tgccctggct gcgctgggct
     3541 gccgcgctgg ccgccgacct gcgccgcgat gccgaacccg ctgccatcgc cgccgcgctc
     3601 gacgcgcgct atgtgccgcc ctcgccgggc gccgacgtgg tgcgcaaccc ggcggtcgcc
     3661 cccaccgggc gcaacatcta cgcgctcgac ccttacggcg tgcccaccgg catggcctgg
     3721 gaaaccggca agcgcgccgc cgaggcgctg ctggcccgct accggcgcga gcagggcgcc
     3781 tggccgcgcg ccattgccct ggtgctctgg ggcacggaca atctgaagac ggcgggcgag
     3841 ggcatcgccc aggcgctctg gctgctcggc gtgcggcccc accgcgacgc cctgggccgc
     3901 gtggccgatg tgcgactggt gccgctggcc gagctgggcc gcccgcgcat ggacgtggtg
     3961 ctgacctgtt ccggcatctt ccgcgatctg ctgcccaacc aactcactct gctcgaccgc
     4021 gccgtgcgcc tggccgccgc cgctgacgaa ccgccggagc agaacttcgt gcgccgcaac
     4081 gccctggccg cgacggagac gggcttgccc ttcgccgagg cggccacgcg tgtgttcagc
     4141 aatgcgccgg gggcctacgg cgccaatgtc aatttccagg tcgagaccgg ggcctgggac
     4201 gacgacgcgg ccctggcgca gaccttcctg gcccgcaaga gcttcgccta cggcctgggc
     4261 ggtgagggcg tggcggcacg cgatctgctg gaaagtgcct tgcggccggt cgatctcgct
     4321 ttccagaaca tcgacagcat cgagaccggg atcagcgaca tcgatcacta ctttgagtac
     4381 ctcggcggcg tgtcgaaggc cgtggagata ctcggcggca ccgcgcccgc cggcttcgtg
     4441 ctcgacgtgc agggcgtggg cgggcgcctg cgcagtctcg gcgagatggt ggcggtggag
     4501 agccgcacca agctgctcaa cccgcgctgg agcgaaggca tgttgcgctt cggctacgag
     4561 ggcgtgcgcg agatcggcgt gcgggtcgcc aacacgctgg gctggagcgc caccacccgc
     4621 gccgtgcccg gctgggtcta cgacggcctg gccgagacct acgtgctcga cgccgcccgc
     4681 cgcgagcaga tggcgaacct caacccgaac gcctaccagg cgctggtggg ccgcctgctc
     4741 gaagccgaag gccgcggctt ctggacgccc gcgccggagg tgagcgccga actgcgccgc
     4801 gtctacggcg aggctgagga tctagttgaa caggtgcgtt aaatgccgtc ccatccacca
     4861 acccataccc acatgcttgc cggcagcttg ctgctgtgtg ccatgctgcc cctgctgatc
     4921 gcttgcggag cagcggcgcc cgccgccccg gcgaccgtgc cgcccgccgc ctcgcccact
     4981 gctgccctgc ctaccgcgcc ccctaccgcc gcgccggcca ctgtcccggc aacggagccg
     5041 cccaccgtcc cggagacggt cgcgcccacc ctggccgcga ccacccctcc gaccacggcg
     5101 ccgcccaccg ccatcccggc gacagcgtcg cccagcggca ccccggccct tgcccgcgtg
     5161 atcaccaacg ccgacggcgg cgggcagttc accttcgcgg tcgggcagac gttctcgttg
     5221 caactgagcg cccgccgctg gtcgcaaccg gttgtggacc cgcagatctt gcgcgccctg
     5281 ccggtcccca cgcccgccgc gcccgatgcc tcccgctggg attaccaggc cgtggcgccg
     5341 ggccgcaccg acctgacgac tgagggcgcc tgccccccgg ccccgcccgg cggcgtgacc
     5401 tgcatgtcca tcgaactgta taagatcacg attatcgtca caccgtgagc agcctacctc
     5461 acccgctgtc actctgagcg cagcgaagag tctcgtctcc gtcagcgggc cgggccaaaa
     5521 atcggtctcg cgtcaggttg acaacggcgg gggcttctgg tataatctgg ggcgtgcctg
     5581 aaacgggcac ggttccgggg agtagctcag ctaggctaga gcgcagcgtt cgggacgctg
     5641 aggtcggagg ttcaagtcct ctctccccga ccacataacc ctgtccaaca ggcccgcggc
     5701 agcgctgtcg cgggcttttt gtcgttccga tgacctttta tcctcaccgc agaggttttc
     5761 gacggtttaa cttcaccgcg aagaaacgaa ggaaacgaag gat
//
LOCUS       DASUYC010001036         2193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1876653, whole genome shotgun sequence.
ACCESSION   DASUYC010001036 DASUYC010000000
VERSION     DASUYC010001036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1876653"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="VF276_16935"
     CDS             complement(<1..841)
                     /locus_tag="VF276_16935"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX6608589.1"
                     /translation="MDTQIAAPAQSAPATPLGLGLVGCGGFGRFCLEAYRAVPGLRLV
                     AASDNDPGRLQATAADFGVQPYADYEALLADPAVQIVAINTPPSLHGAQAVAAARAGK
                     HIFCEKPLATNLDDATAVLRAVRDASVVLSVDYVMRWNPLYWFLKHLQTLRVGDSPLL
                     GPLQRYALENDAGDEQLAPAHWFWDPAISGGIFVEHGVHFFDACAWLAGTPVTVVQGL
                     SLPRREPAGGNGGALLIDTVAATAVHTGGTIASYYHAFTHANRAEYQSITLDWGFAHG
                     VLYG"
     gene            complement(1006..>2193)
                     /locus_tag="VF276_16940"
     CDS             complement(1006..>2193)
                     /locus_tag="VF276_16940"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608590.1"
                     /translation="PLFVVAAHHHYAATGDGEYLSLVYPAVRGACDWLLGQRRDGLVW
                     CDVEGTDVWGNATWRNIIPGYRLAGAVTEINSLAVWALACAAALAEAAGTVDDAARWR
                     QARADLLAAMREQLLAPETGTYLLNRDETGRHLTLTADLVFPVLGGVAEGEQARGILD
                     RLYSPQFYTPFGIHTVGQGEAEYHPSYGYGLMGGLWPNLTAWVAYAGRRQYPARLTEM
                     MDHLYALCEVPEPRAGGHMAPGEFPEWFHGETYESLGMAMSPWMPPTYLWLGLEGLAG
                     IEAQPGGLRVAPNLPPDWTWFGLRNLPYRGGQVSLFAHDGRLYSTLAVDTDWPLEQFE
                     RDATDEIAVVGPLYALALAGAQTGVVLVGTVADVRGAIRWGSAVQSVDLRAGQAVVLR
                     FDL"
BASE COUNT          324 a          773 c          777 g          319 t
ORIGIN      
        1 agccatacag cacgccgtgg gcgaagcccc agtccagcgt gatgctctgg tactcggcgc
       61 ggttggcgtg cgtgaaggcg tggtagtagc tcgcgatggt gccgcctgta tgcacggcgg
      121 tggcggcgac agtatcgatc agcagcgcgc cgccgttacc gcccgccggc tcgcgccggg
      181 gcaggctgag gccctggacc accgtgaccg gcgtacccgc cagccacgcg caggcgtcga
      241 agaagtggac gccatgctcg acgaaaatgc ccccgctgat ggccgggtcc cagaaccagt
      301 gggccggcgc cagttgctcg tcgcccgcgt cgttctccag cgcatagcgc tgcaacggcc
      361 ccagcagcgg gctgtcgccc acgcgtagcg tctgcaggtg tttgaggaac cagtagagcg
      421 ggttccagcg catgacgtag tcgacgctca gcacgacgct cgcatcgcgc acggcgcgca
      481 acaccgcggt agcgtcgtcc aggttggtcg ccagcggctt ctcgcagaag atgtgcttgc
      541 ccgcccgcgc ggcagccacc gcctgcgcgc cgtgcagcga cggcggcgtg ttgatcgcca
      601 cgatctgcac cgccgggtcg gccagcagcg cctcgtagtc ggcgtacggc tgcacgccga
      661 agtcggcggc ggtggcctgg aggcggcccg gatcgttgtc gctggcggcg accaggcgca
      721 ggccgggcac cgcccgatag gcttccaggc aaaaccggcc aaacccgccg cagcccacca
      781 accccaggcc tagcggcgtc gccggggcgc tttgcgcggg cgcggcgatt tgcgtgtcca
      841 tgcgtattcc cactcctgtg ctacgccgcc gcgctcggca gcgcctgagt agatcatccg
      901 agtttgtcac tctgagcgca gcgaagagtc tcgtctggcg gggtaacgga gattcttcgc
      961 tgcgctcaga atgaccggtt gttggtgaca agctgtggcg cgagcctaga gatcgaagcg
     1021 cagcacgacc gcttgaccgg cccgcaggtc cacggattgg accgcgctgc cccagcggat
     1081 cgcgccccgc acatcggcta ccgtgcccac aagcaccacg ccggtctgcg cgcccgccag
     1141 ggccagcgcg tagagcgggc cgacgacggc gatctcgtcc gtggcgtcgc gctcgaactg
     1201 ttccagcggc cagtcggtgt ccaccgccag cgtgctgtag agtcgcccgt cgtgggcgaa
     1261 caggctgacc tgcccgccgc gatagggcag gttgcgcagg ccgaaccagg tccagtcggg
     1321 cggcaggttg ggcgcgacgc gcaggccgcc gggctgcgct tcgatcccgg ccagcccttc
     1381 gaggcccagc cagagatagg tcggcggcat ccagggcgac atcgccatgc ccaggctttc
     1441 gtaggtctcg ccgtggaacc actccggaaa ttcgcccggc gccatatggc ccccggcgcg
     1501 cggctcgggc acttcgcaga gcgcgtagag gtggtccatc atctcggtca ggcgcgcggg
     1561 gtactgccgc cgcccggcgt aggccaccca ggcggtgagg ttgggccaca gcccgcccat
     1621 caggccgtag ccgtaggagg ggtggtactc ggcctcgccc tggcccaccg tgtggatgcc
     1681 gaacggggtg tagaactgcg ggctgtagag ccggtccagg atgcctcgcg cctgctcgcc
     1741 ttcggccacc ccgcccagga cgggaaacac caggtccgcg gtcagcgtca ggtggcggcc
     1801 cgtctcatca cggttcagca gataggtgcc ggtttcgggc gcgagcagtt gctcgcgcat
     1861 ggcggccagc aggtcggcgc gcgcctgccg ccagcgggcg gcgtcgtcca ccgtgcccgc
     1921 cgcctctgcc agcgcggcgg cgcaggccag cgcccagacg gccagactgt tgatctccgt
     1981 caccgccccg gccaggcggt agccggggat gatgttgcgc caggtggcgt tgccccacac
     2041 gtcggtgccc tcgacatcgc accagactag cccatctcgc cgctgcccta gcagccagtc
     2101 gcaggccccg cgcacggcgg ggtagaccag gctgagatac tcgccgtcgc cggtggccgc
     2161 gtagtggtgg tgggcggcca cgacgaagag ggg
//
LOCUS       DASUYC010001037         3303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_213199,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001037 DASUYC010000000
VERSION     DASUYC010001037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213199"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VF276_16945"
     CDS             <1..181
                     /locus_tag="VF276_16945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608591.1"
                     /translation="GRFAILRCIHDNPSLPLDVSRSFPRMLARTRPASKREAGPRPAI
                     VIHREGAKDAKKKLL"
     gene            375..1547
                     /locus_tag="VF276_16950"
     CDS             375..1547
                     /locus_tag="VF276_16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020529460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6608592.1"
                     /translation="MASAPVRAAERAFLRAPFPAVRPQLDALRRDVQAAGLWAPQIPV
                     VFGGLGLSLLEFARVSEELGRSPIGHYLCNCQAPDSGNMELLIAHGTPAQQQEWLHPL
                     VRGEIRSCFAMTEPDYPGSNPVWMATRAVRDGADYVIDGHKWFTSAADGAAFAVVMAV
                     TNPDAPRPHERASQIIVPADTPGFALVRNLPVMGEPGAGYAGHAEVRYTGCRVPQSNR
                     LGPEGAGFALAQARLGPGRIHHCMRWIGICERAFDLMCARAAGRELAPGVPLGRRETV
                     QNWVAESRAEINAARLLVLDAAAKIDQAGAQAARTEISIIKFFVAGVLQQVLDRAIQV
                     HGALGMTDDTPLAYWYRHERGARIYDGPDEVHKAAVARQILRGYGLDVGRADGERS"
     gene            1551..2633
                     /locus_tag="VF276_16955"
     CDS             1551..2633
                     /locus_tag="VF276_16955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEX6608593.1"
                     /translation="MADWLDQPQAVRPGEELDAARLAAYLATAAPALSGPIRVEQFPQ
                     GYSNLTYLLRLGGREVVLRRPPFGVGKGSAHDMAREYRTLAGVARVYPRVPHPLVYCA
                     DETVLGAPFYLMERVPGVILRARPPAGLALPPATMRRLAEATIDNLAAIHALDYEAAG
                     LGDLGHPAGYAERQVQGWTARYARARTDDIPEIEQAAAWLAAHIPPEAPPALLHNDYK
                     YDNLVLDPADLPRIRAVLDWEMATLGDPLMDLGTTLGYWVQADDPPELQAMRFGPTTL
                     PGNLSRAQLVQRYAGASGRDVGNIGFYYVYGLFKIAVIAQQIYARYRQGVTHDARFAG
                     LLPAIQALGRTAVRAMTSGRLAGLDR"
     gene            complement(2666..>3303)
                     /locus_tag="VF276_16960"
     CDS             complement(2666..>3303)
                     /locus_tag="VF276_16960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX6608594.1"
                     /translation="TMARFAELNVAFPVVLVDRSLKNGDADAVLLDNVNAAMELTTHL
                     LEQGYRRIAALLGDTGTTGRERQRGYEAALREYGLAPVSALVRHVRPRIETGRAVTLE
                     LLDFDPPCDAILTGNSLLTAGALQAIRERNLAIPRDVALAGFDETIWSSLVEPAITTL
                     AQPTAEIGKTATELLLQRIAAPQRAPRKVILQGTLHVRDSSAARVPASTGG"
BASE COUNT          487 a         1209 c         1135 g          472 t
ORIGIN      
        1 gggtcgattc gccattctcc gctgtattca cgataaccca agcctccctc tcgacgtaag
       61 ccgatcattc ccgcgtatgt tagcacgcac gcgcccggca agcaagcggg aggccggccc
      121 gcgcccggcg attgttattc accgcgaagg cgcgaaggac gcgaagaaaa aactgctatg
      181 aataatcttc gcgttcttcg cgccttcgcg gtgcacttgt gagcgcctgc cgtaggatac
      241 cgggcggcgc ttgacgccgg gcggggcgcg tgctatgctg agggccgagc ctggctgccg
      301 ccgctatgga ggacatcttg gaaccggccc catcgccccg cctggacacg atcttgcccg
      361 ccctgcgcgc gttcatggcg tccgccccgg tgcgcgccgc cgaacgcgcc ttcctgcgag
      421 cgccgttccc cgccgtgcgc ccgcaactcg acgccttgcg ccgcgacgtg caggcggcgg
      481 gactgtgggc accgcagatc ccggtcgtgt tcggcggcct gggcctgtcg ctgctggagt
      541 tcgcccgcgt cagcgaggag ttgggccgca gccctatcgg ccactacctc tgcaattgcc
      601 aggcgcccga ctcgggcaac atggaactgc tgatcgcgca cggcaccccg gcgcagcagc
      661 aggagtggct gcacccgctg gtgcgcggcg agatccgcag ttgcttcgcc atgaccgagc
      721 ccgactaccc cggttccaac cccgtctgga tggcgactcg cgccgtgcgc gacggcgccg
      781 actacgtgat cgatgggcac aagtggttca cttcggcggc ggacggcgcg gcattcgccg
      841 tcgtcatggc cgtgaccaac ccggacgcgc cccggcccca cgagcgcgcc agccagatca
      901 tcgtgccggc cgatacgccg ggcttcgcgc tggtgcgcaa cctgccggtg atgggcgaac
      961 cgggcgcggg ctacgccggc cacgccgagg tgcgctatac aggctgccgc gtgccgcaaa
     1021 gcaaccggct ggggccggag ggcgccggat tcgcgctggc ccaggcgcgg ctggggccgg
     1081 ggcgcatcca ccattgcatg cgctggatcg gcatctgcga gcgggccttc gacctgatgt
     1141 gcgcgcgcgc cgcggggcgg gaactcgcgc cgggcgtgcc cctgggccgc cgcgagacgg
     1201 tgcagaactg ggtcgccgag agccgcgccg agatcaacgc cgcccggctg ctggtgctcg
     1261 acgccgccgc caaaatcgac caggccggcg cccaggccgc ccgcaccgag atctcgatca
     1321 tcaagttctt cgtggccggc gtgttgcagc aggtgctcga ccgggccatc caggtccacg
     1381 gcgcgctggg catgaccgac gacacgccgc tggcctactg gtatcgccac gagcgcggcg
     1441 cccgcatcta cgacgggccg gacgaggtgc acaaggccgc cgtggcccgg cagatcctgc
     1501 gcggctacgg cctggacgtg ggccgcgcgg acggcgagag gagctagcgc gtggccgatt
     1561 ggctggacca gccgcaggcc gtgcggcccg gcgaggaact cgacgcggcg cggctggccg
     1621 cctacctggc caccgccgcg cccgccctgt ccgggccgat ccgggtggaa cagttcccgc
     1681 agggctactc gaatctcacc tacctgctgc gcctcggcgg gcgcgaggtg gtgttgcggc
     1741 gcccgccctt cggcgtgggc aaaggctcgg cccacgacat ggcccgcgaa taccgcacgc
     1801 tggcgggggt ggcgcgggtc tatccgcggg tgccgcaccc gctggtctat tgcgccgacg
     1861 agaccgtgct gggagcgccg ttctacctga tggagcgcgt gcccggcgtg atcctgcgcg
     1921 cccgcccgcc cgccgggctg gccctgcccc cggccaccat gcgccgcctg gccgaagcga
     1981 ccatcgacaa ccttgccgcc atccatgccc tggactacga ggccgcgggc ctcggcgacc
     2041 tgggccaccc ggcgggttac gcggagcggc aggtgcaggg ctggacggcg cgctacgccc
     2101 gcgcccgcac cgacgacatc ccggagatcg agcaggctgc cgcttggctg gccgcccaca
     2161 tcccgccgga ggcgcccccg gccctgctgc acaacgacta caagtacgac aacctggtgc
     2221 tcgaccccgc cgacctgccg cgcatccgcg ccgtgctcga ctgggagatg gcgaccctgg
     2281 gcgacccgct gatggacctg ggcaccacgc tcggctactg ggtgcaggcc gatgacccgc
     2341 ccgagttgca ggccatgcgc ttcggtccca ccaccctgcc cggcaacctg agtcgcgccc
     2401 aactggtgca gcgctatgcc ggggcgagcg ggcgcgacgt gggcaacatc ggcttctatt
     2461 acgtctacgg cctgttcaag atcgccgtca tcgcccagca gatctatgcc cgctaccggc
     2521 agggcgtcac ccacgacgcg cgctttgccg gcctcctccc cgccatccag gcgctgggtc
     2581 gcacggcggt gcgcgccatg acaagcggac gcctcgccgg gctggacaga taacgccggg
     2641 aacagaacga ccgggggaag ccgggctatc ctccagtgct ggcgggcacc cgcgcggcgc
     2701 tcgaatcccg cacatgcaag gttccctgca aaatcacctt gcgcggcgcc cgctgcggcg
     2761 cggcgatgcg ctgcaacagc aactcggtcg ccgtcttgcc gatctccgcc gtgggctgcg
     2821 ccagcgtggt aatcgccggt tcgaccaggg aagaccagat cgtttcgtcg aagccggcca
     2881 gggccacgtc ccgcggaatg gccaagttcc gttcgcggat cgcctgcaag gcccccgccg
     2941 tcagcagact gttgccggtc aggatagcat cgcacggcgg gtcgaaatcg agcaattcca
     3001 gcgtgacggc gcgcccggtt tcgatgcgcg ggcggacatg gcggaccagc gcggaaacgg
     3061 gcgccaggcc gtactcgcgc agggccgctt cgtagccgcg ctgccgctcg cggcccgtgg
     3121 taccggtatc gcccaggagc gcggcaatgc gccggtagcc ctgctccagg agatgggtgg
     3181 taagctccat ggcggcgttc acattgtcca gcaacacggc atcggcatcc ccgttcttca
     3241 gcgaacggtc gaccagcact acgggaaagg ccacgttcag ttccgcgaac cgcgccatcg
     3301 tgc
//
LOCUS       DASUYC010001038         3958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_993496,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001038 DASUYC010000000
VERSION     DASUYC010001038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993496"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VF276_16965"
     CDS             <1..407
                     /locus_tag="VF276_16965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6608595.1"
                     /translation="QAETAAPPPLFPDFRAMSDEERLATARTMNPHWHPDELPPWAES
                     KAQFDPAVYRYFYTVPDFPWEEVLAHIECPALLLTGDPAAGAVVTPEVAREALGLLQH
                     GQLVQIAGSGHNIHRDAYEPALQAVRAFLQAH"
     gene            542..1525
                     /locus_tag="VF276_16970"
     CDS             542..1525
                     /locus_tag="VF276_16970"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6608596.1"
                     /translation="MEYTKLGNTGLDVSRICLGCMGFGDATRWIHQWVLDEEHSRPIV
                     KHAIELGINFFDTANVYSLGASEEILGRALRDFANRDEVVIATKVHGRMHEGPNGAGL
                     SRKAILSEIDHSLQRLGMDYVDLYIIHRWDYQTPIEETMEALHDVVRAGKARYIGASA
                     MFAWQFQKALYVAERHGWTRFVSMQDHLNLIYREEEREMLPLCRAEGIGVTPYSPLAS
                     GRLTRDWSADSTLRAATDEVARSKYDATAGTDRQIVERVAELAEKHGVPRVHIALAWL
                     LQKQPVAAPIIGATKTAHLDDAAGALAVHLSEEEVAYLEAPYVPHRIVGHQ"
     gene            1561..3237
                     /locus_tag="VF276_16975"
     CDS             1561..3237
                     /locus_tag="VF276_16975"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6608597.1"
                     /translation="MSTVSPARHSAAGSLAPGASMWSPLRRPAFRSVWIAWVVASTGN
                     WMQIAGAAWLMTSLTSSPLLVALMQTATSLPVFLVGLPAGALADIVDRRKLLLATQSW
                     NLLAALTMGLLTLAGLMSAWTLLAFTFLMGLGTALTGPAWQALLPELVEREDLQSAVA
                     LNAGGFHAAQAVGPALGGLVVGTAGPGAAFLLNAASLLDMLRAIYRWRPARTASDLPP
                     EDMLGASLAGMRYVRDAPALQAVLVRIGAFTLGASALWSLLPVVARRELGLDPTGYGI
                     VLGSLGAGAVCGVLLLPRLRRALSLDRLTAAATLVFAGATLAAAYLRFAPLVVLTTAA
                     GGLAWLTVMSCVGFAAQIAVPAWVRARALSVYLLVFQGLLAAGSLAWGALAQQFGTAT
                     ALTAAAVALAGGLAATGRWPLHQVQRLDLNPSGHWPGPQVAITPELEDGPVLVTVEYR
                     VPTERADEFLAAMDAMRRFRRREGAIRWDLFHDLGDPDRYLETFLVTSWAEHMRQHTR
                     VTVEDQAIEARAFSFLQPGVAPVAAHLIAVHLANGRPAAGPPGAELPIDI"
     gene            3260..>3958
                     /locus_tag="VF276_16980"
     CDS             3260..>3958
                     /locus_tag="VF276_16980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6608598.1"
                     /translation="MQQRPLGRGAAALEVSALGLGCMGMSVNYGPPRDRQEMIALVAA
                     AVDQGLTFFDTAESYGPFVNEELVGEALAPFRGHVVIATKFGWHFGPNGERLGRSSKP
                     EQIKQAVEGSLGRLRVETIDLYYQHRVDPEVPIEDVAGTVKDLIGAGKVKHFGLSEAS
                     AQTIRRAHAVQPVTAVQSEYSLWARDPEAEVLPACAELGIGFVPWSPLGQGFLTATVD
                     PAGAFDASDIRSRFP"
BASE COUNT          613 a         1326 c         1383 g          636 t
ORIGIN      
        1 cccaggcgga gacggcagcc cccccaccgt tgttccctga cttccgggcc atgtccgacg
       61 aagagcggct cgccacggcc agaaccatga atccacactg gcatcccgac gaattgccac
      121 cctgggccga gtcgaaagcg cagtttgacc cggcagtgta ccggtatttc tatacggtcc
      181 ccgacttccc ctgggaagaa gtgctcgcgc atattgaatg tccggcactg ctgctcacag
      241 gcgatccggc ggcaggcgct gtggtgacac cggaggtggc gcgagaagcg ctggggctgt
      301 tgcagcacgg gcaactcgtg cagatcgcgg gcagcgggca caatatccat cgcgacgcct
      361 acgagccggc ccttcaggcg gtacgagcct tcctgcaggc gcactaaagg ggcagggagg
      421 cacccgctga gtagggttgg cccggcaggg caaagcgccg cgacagtatc caacatcggc
      481 gcctggatgc cgagcgccgg cggggcctgg ctgttgcacg caggtaagag aggaaaacga
      541 catggaatac acgaagctcg ggaataccgg cctggatgtg tcgcgcatct gcctcggctg
      601 catggggttc ggggacgcga cccgctggat ccaccaatgg gtgctggacg aggaacacag
      661 ccggccgatc gtcaagcacg ccatcgaact ggggatcaat ttcttcgata ccgccaacgt
      721 ctattccctg ggggcgagtg aggagatcct gggccgcgcg ctgcgggact tcgccaaccg
      781 cgacgaagtg gtcatcgcca ccaaggtgca cggccgcatg cacgaggggc cgaacggcgc
      841 cgggctatcg cgcaaagcga tcttaagcga gatcgatcat agcctgcaac ggctggggat
      901 ggattacgtg gacctctaca tcatccatcg ctgggattac cagaccccga tcgaggagac
      961 gatggaggcg ctgcacgacg tggtgcgggc gggtaaggcg cgctatatcg gcgcctccgc
     1021 catgttcgcg tggcagttcc agaaggcgct gtacgtcgcg gagcggcatg ggtggacgcg
     1081 cttcgtctcg atgcaggacc acctgaacct gatctaccgc gaagaggagc gggagatgct
     1141 gccgctgtgc cgggcggaag ggatcggggt gacgccgtat agtcccctgg cctccggccg
     1201 cctgacgcgg gactggtcgg cggacagcac gcttcgcgcc gccaccgatg aagtcgcgcg
     1261 cagcaagtac gatgcaaccg ccggcaccga tcgccagatc gtggagcgag tggcggaact
     1321 ggcggagaag catggggtgc cgcgcgtgca tatcgcgctg gcctggctgt tgcagaagca
     1381 gccggtggcg gcgccgatca ttggggccac gaagaccgcg catctggacg atgccgcggg
     1441 cgcgctggcc gtacacctga gcgaggaaga ggtcgcctac ctggaagcgc cgtatgtgcc
     1501 gcatcggatc gttgggcatc agtaagtacg cagacagcgc agagcgcagg agggcacacg
     1561 atgagtacgg ttagcccggc ccggcacagc gccgccggca gtctcgcgcc gggtgcttcg
     1621 atgtggagcc cgctgcgccg cccggccttt cgctcggttt ggatcgcctg ggtggtcgcc
     1681 agcaccggta actggatgca gatcgccggc gccgcctggc tgatgacctc gctcacgtcg
     1741 tcgccgctgc tggtggcgct catgcagacc gccaccagcc tgcctgtctt cctggttggc
     1801 ctgcccgccg gggcgctggc cgacatcgtc gaccggcgca agctcttgct cgccacccag
     1861 tcctggaacc tcctcgccgc cctcaccatg gggttgctga ccctggcggg acttatgtcg
     1921 gcctggaccc tgctggcgtt caccttcctg atgggcctgg ggaccgcgct gaccggccca
     1981 gcgtggcagg cgctgctccc cgagctcgtc gaacgcgagg atctccaatc cgccgttgcg
     2041 ctgaacgccg gcggatttca cgcggcgcag gccgtggggc cggccctcgg cggcctggtg
     2101 gtgggcacgg cagggccggg cgccgccttc ctgctgaacg cggcctcctt gctggacatg
     2161 ctccgggcga tctatcgctg gcggcccgcc cgaaccgcga gcgatctccc gcccgaagac
     2221 atgctggggg cctcgctcgc gggtatgcgc tacgtgcgcg atgccccggc gctccaggcc
     2281 gtcctggtgc gcatcggcgc cttcaccctg ggcgccagcg ccctctggtc cctgctgccg
     2341 gtggtggccc ggcgcgagct cggcctggac ccgaccgggt atgggatcgt cctgggcagc
     2401 ctcggtgccg gcgccgtgtg cggggtcctg ctgctgccgc gcctgagacg agcgctctcg
     2461 ctggaccgct tgaccgctgc agccacgctc gtgttcgccg gggccacgct ggccgcggcc
     2521 tacctgcgct tcgcgccact ggtcgtgctg accaccgccg cgggcgggct ggcctggctg
     2581 acagtgatgt cctgcgtggg cttcgcggcg cagatagcgg tcccggcctg ggtgcgcgcg
     2641 cgggccctga gcgtctacct gctggtattc cagggcctgc tggcggcggg cagcttggcc
     2701 tggggcgctc tggcccagca attcggcacg gccaccgccc tcacggcggc cgccgtggcc
     2761 ctggccggcg ggttggcggc gaccgggcgc tggccgctgc atcaggtcca gcgcctcgac
     2821 ctgaatccct ccgggcactg gccgggcccc caggtagcga ttacgccgga gctggaggat
     2881 gggccggtgc tcgtcacggt cgaataccgg gtgcccaccg agcgcgcgga cgagttcctc
     2941 gcggccatgg atgcgatgcg ccgcttccgc cgtcgcgagg gcgccatccg ctgggacctg
     3001 ttccacgacc tgggcgaccc cgaccgctac ctggagacct tcctggtcac ctcgtgggcc
     3061 gagcacatgc gccagcatac ccgcgtcacc gtggaagatc aggccatcga agcccgcgcc
     3121 ttctccttcc tgcagccggg cgtcgctccg gtcgccgcgc acctcatcgc cgtccacctc
     3181 gccaatggcc ggcccgccgc ggggccgccc ggcgctgagc tacccatcga tatttagcgc
     3241 actatagagg agtagaacaa tgcaacagcg tccactgggc cggggcgccg ccgccctgga
     3301 agtctcggcg ctcggcctcg gctgcatggg catgagcgtc aattacggcc caccccgaga
     3361 caggcaagaa atgatagccc tcgtggcggc ggccgtcgat cagggcctca cctttttcga
     3421 taccgccgaa tcctacggcc cgttcgtcaa cgaagaactg gtcggcgaag cgctggcgcc
     3481 tttccgcggg cacgtggtga ttgccaccaa attcggctgg cattttggac ccaacgggga
     3541 gcggttgggc cggtcgagta agccggagca gatcaagcaa gcggtcgaag gctcgctggg
     3601 gcgcctgcgg gtcgaaacca ttgatctgta ctatcaacac cgggtggacc cggaggtgcc
     3661 gattgaagac gtggccggca cggtcaagga cctgatcggg gcaggcaagg tgaagcactt
     3721 tggtctttcc gaggcgagtg cgcagacgat ccggcgggcg cacgcggtgc agccggtcac
     3781 ggccgtgcag agcgagtatt cgctatgggc acgcgacccg gaagcggaag tgctgccggc
     3841 gtgcgcagaa ttgggcatcg gcttcgtgcc ctggagcccg ctggggcagg gctttctcac
     3901 cgcgacggtg gacccggccg gcgcgttcga cgcgtccgac attcgctccc ggttcccg
//
LOCUS       DASUYC010001039         2403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_870771,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001039 DASUYC010000000
VERSION     DASUYC010001039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_870771"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1342
                     /locus_tag="VF276_16985"
     CDS             <1..1342
                     /locus_tag="VF276_16985"
                     /inference="COORDINATES: protein motif:HMM:NF013425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX6608599.1"
                     /translation="DGVRLAALLDEAAAVVDALLGFGLQRDITGELAAIIAAVQGAVE
                     RRARTARPLPVFAVDIPTGVDSDTGRVRGVALPAQVTITLGVAKRGLYLYPGAACAGE
                     IILGDIGVGDLTDNVQVTNTTAEQVRGLLPARPADANKGTFGSVLIVAGSLNYIGAAV
                     LSTLGAMRIGVGLATLATPIELLPIVAAKLTECTFLPLPADMGILTERSVKPLFDALT
                     ERQYKALLVGNGLGHEKETLNFMRGVLQDKAVTAPARARAERHVGFGLPRHAEPSKPE
                     EAEEDKTPGLPPLVLDADGLNLLHQIEGWAERLPADTILTPHPGEMARLLDSDVETVQ
                     ADRVGTAQRAAAEWKAIVVLKGANTVIAAPDGRVAVNPAASAALATAGTGDVLAGCIA
                     GLLAQGLAPFDAAVAGVFVHGQAGLLVAEELGDAGVLAGDVAEALPHALREIKG"
     gene            1375..>2403
                     /gene="alr"
                     /locus_tag="VF276_16990"
     CDS             1375..>2403
                     /gene="alr"
                     /locus_tag="VF276_16990"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX6608600.1"
                     /translation="MPETEDGRAAPRWRGRPVWIEIDLDAVAENVRRIAAWVGPGTAV
                     MAVVKADGYGLGAAQIGTAALAGGATWLAVASVDEGVQLREAGIDSPILVLGPTTPWE
                     TSRAVAARLTLTVNSVEVARALSAAARQVRVRAPVHLKLDTGLNRYGRDPDELVTLAQ
                     TLAAMPGLRLEGLYTHFANAGEPGDDYAARQLETLLSVQQRLARQRITFALVHAASST
                     GILELPESHLDLVRAGIMLTGHYPAPDSSHTVRLASVVTVHARIARLFTVPAGATVGY
                     GRTYRAPGPRTLALVPMGYADGYHRALSNRGYMLVGGRRAPVVGRVSMDQTTIDVTGI
                     PGVREGDPV"
BASE COUNT          342 a          832 c          896 g          333 t
ORIGIN      
        1 ggacggcgtg cgcctggccg ccctgctgga cgaggctgcc gccgtggtcg atgcgctgct
       61 cggcttcggc ttgcaacgcg acatcaccgg cgaactggcg gccattatcg ctgccgtcca
      121 gggtgcggtc gagcggcggg ctagaacggc ccgccccttg ccggtcttcg cggtggacat
      181 acccacaggc gtcgattcag acacgggtcg cgttcgcggg gtggccctgc ccgcccaggt
      241 cacgattacg ctgggagtgg cgaagcgcgg gctgtacctg tacccaggag cagcctgcgc
      301 gggggaaatt atcctggggg atataggagt cggagacttg actgataacg tacaggtaac
      361 gaacacgacc gccgagcagg tgcgcggctt gctgcccgcc cgcccggcgg acgcgaacaa
      421 aggcaccttc ggcagcgtgc tgatcgtggc cgggtcgctg aactacatcg gcgcggcggt
      481 gctgtccacg ctgggtgcga tgcgcatcgg cgtgggcctg gcgacgctgg cgacgccgat
      541 tgagctgctg cccatcgtgg ccgccaagct gacggaatgc accttcctgc ccctgcccgc
      601 cgatatgggc atcctgaccg aacgctcggt caagccgttg ttcgacgcgc tgaccgagcg
      661 acagtataag gcgctgctgg tgggcaacgg gctgggccac gagaaggaaa cgctcaattt
      721 tatgcgcggc gtgttgcagg ataaggccgt caccgccccg gcgcgcgcac gcgcggagcg
      781 ccacgtcggc ttcgggctgc cccggcacgc cgagccgtcc aagccggaag aggccgagga
      841 ggacaaaact cccggcctgc cgcccctcgt gctggacgcc gacgggctga acctgctgca
      901 ccagatcgag ggttgggcgg agcgcctgcc cgcggacacc atcctgacac cgcaccccgg
      961 cgagatggcc cggctgctgg acagcgacgt ggagacggtg caggccgacc gcgtgggcac
     1021 ggcgcagcgc gccgccgccg agtggaaggc catcgtggtg ctcaagggcg ccaacacggt
     1081 gatcgccgcg cccgatggcc gggtggccgt caatccggcg gcgtccgccg cgctcgccac
     1141 ggcgggcacg ggcgatgtgc tggccgggtg tatcgccggg ctgctggccc agggcctggc
     1201 gccgttcgac gccgccgtgg ccggggtttt cgtccacggc caggcggggc tgctggtagc
     1261 cgaggaactc ggcgatgccg gcgtcctggc gggcgatgtg gccgaggcgc tgccccacgc
     1321 gttgcgcgag atcaagggct gatgatgaac ggggccgggc cggccacgag ggatatgcct
     1381 gagacggagg atgggcgcgc cgcgccgcgc tggcggggcc gcccggtctg gatcgagatc
     1441 gacctcgacg ccgtcgccga gaacgtgcgg cggatcgcgg cgtgggtcgg tcccggcacg
     1501 gcggtgatgg ccgtggtcaa agccgacggc tacggcctgg gcgccgcgca gatcggtacg
     1561 gcggcgctgg ccggcggcgc cacctggctg gccgtggcct cggttgacga gggcgtgcag
     1621 ttgcgcgagg ccgggatcga ttcgcccatc ctggtgctgg ggccgaccac accctgggaa
     1681 acgagccgcg ccgtggccgc ccggctcacg ctcacggtca acagcgtgga ggtggcgcga
     1741 gcgctgtcgg cggcggcgcg gcaggtgcgg gtgcgcgcgc cggtccacct caagctcgac
     1801 acgggactga atcgctacgg gcgcgatccc gacgagctcg tgaccctggc gcaaacgctg
     1861 gccgccatgc cggggctgcg cctggagggc ctctacaccc acttcgccaa cgccggcgaa
     1921 cccggcgacg actacgccgc ccggcagctt gaaacgctgc tgagtgtgca gcagcgcctg
     1981 gcccgccagc gcatcacctt cgccctcgtc catgctgcga gcagcacggg catcctggaa
     2041 ctgccggaga gccacctgga tctggtgcgc gccgggatca tgctcaccgg gcactacccg
     2101 gcgcccgact ccagccacac cgtccggctg gcgtccgtgg tgacggtcca tgcgcgcatc
     2161 gcccgcctgt tcaccgtgcc cgcgggggcc accgtcggct atgggcgcac ctaccgggcg
     2221 ccgggcccgc gcaccctcgc gctggtgccc atgggctacg ccgacggcta ccaccgcgcg
     2281 ctgtccaacc gcggctatat gctcgtgggc gggcggcgag cgccggtcgt ggggcgcgtc
     2341 agcatggacc agacgacgat cgacgtgacc ggcatccccg gcgtgcgcga gggcgatccc
     2401 gtc
//
LOCUS       DASUYC010001040         2967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1793366, whole genome shotgun sequence.
ACCESSION   DASUYC010001040 DASUYC010000000
VERSION     DASUYC010001040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1793366"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..1047
                     /locus_tag="VF276_16995"
     CDS             190..1047
                     /locus_tag="VF276_16995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6608601.1"
                     /translation="MIIDFHAHVFPDEVRRDRDRFRARDAWFATLYARAEQKLARAED
                     VIAAMDGAGVDKTVLVGFPWRDPGICALHNDYMIDCVRRYPDRLLAMAIVQPAAGAAA
                     GRELGRCLEAGLVGCGELGPDGQGFRLDSPHDLGPIAEPLLAYRRPLLTHSSEPLGHL
                     YPGKGTVWPGLIYQAALNFPDLLIVAAHWGGGLPFYELMPEVGAALRNVYYDSAASTY
                     LYRFDIFRQVIALAGCERILWATDYPLLGQARFLARTRDAGLSENELAEVLGGNAARL
                     LRLESPEPL"
     gene            1086..1931
                     /locus_tag="VF276_17000"
     CDS             1086..1931
                     /locus_tag="VF276_17000"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX6608602.1"
                     /translation="MALLGQLEEFHLQHIVALLQVERQTGELMLEQDGQRACLYFQDG
                     AIVHAVAGKETGYEAALAPFLWTSGKFHFEAYEPEVEPTITAGNAAIVAAGRRLAVEA
                     EEVRRSVTSMNMLVHLVPQVDTPAGQINLSFSEWRFLTLVDGRRDLAAIGAQLKQADF
                     AVRLIANRLVKNHLIDLRDPRQTMLRMTAMPTSLDLRPPADPLLALMDDLTLDMLLAN
                     HRDRVARARARVQVLTSDDRSLVLAVEGRPDLADRLLLSEVVLARLGVPRNATVHVRL
                     LDDRA"
     gene            2186..>2967
                     /locus_tag="VF276_17005"
     CDS             2186..>2967
                     /locus_tag="VF276_17005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608603.1"
                     /translation="MSDMGADPVAYEWLRLGVRAALEDNREDARYYFVQAVRTDLNFA
                     RAWLYLGGVADDPALTLSCLQKVLRLDPSETQARAGLVWAREKLGLLQPLLPLLSSMS
                     QADLLPQPAPAATVQAPAAPPAPPRTLLWGGLPAPTLPSMPAIATPPPAAQAAPAPRA
                     VPPPAAPEAPPQEEEDSVARLLRRGVRAAVEGNRGRARYYFVKAIAVDPGNTRAWLYF
                     GGVAEDPSTTLACMERVLRDEPDNAQARAGADWAVRRLGINAP"
BASE COUNT          438 a         1023 c         1013 g          493 t
ORIGIN      
        1 cgcgttgatt ctccccgttt tatgcggcgg atcatggggc ctgctgctga ccctcattct
       61 catccacgat tagcggagac ggggtaacgt acttattcct gacggctgac ggctgacggc
      121 tgaccactga ccactgacca ctgaccactc gttcttgaca agcccccggc tttggcataa
      181 actggcgcca tgatcatcga tttccacgcg cacgtcttcc ccgacgaggt gcggcgggat
      241 cgggatcggt tccgggcgcg agatgcctgg ttcgccacgc tctacgcccg cgcggagcaa
      301 aagctggccc gcgccgaaga cgtgatcgcg gcgatggacg gcgccggggt cgataagacg
      361 gtgctggtcg gctttccctg gcgcgatccg ggcatctgcg cgctgcacaa cgactacatg
      421 atcgactgtg tgcggcgcta tccggaccgg ctgctggcga tggcgattgt gcagcccgca
      481 gcgggggcgg cggcggggcg cgaactgggc cgctgcctgg aggcggggct ggtcggctgc
      541 ggcgaactgg ggcccgacgg gcaggggttt cggctcgaca gcccgcacga cctcggcccc
      601 atcgccgagc cgctgctggc ctaccggcgc ccgctgctga cccacagcag cgagccgctg
      661 ggccatctct atccgggcaa agggaccgtt tggccgggac tcatctacca ggcggcgctg
      721 aatttccccg acctgctcat cgtcgcggca cactggggcg ggggcttgcc gttctatgaa
      781 ttgatgccgg aagtgggcgc cgcgctgcgc aatgtgtatt acgatagcgc ggcttcgacc
      841 tatctctacc ggttcgatat tttccgccag gtcatcgccc tcgccgggtg cgagcggatt
      901 ctgtgggcga ccgactaccc gttattgggc caggcgcgct ttctggcgcg gacacgtgac
      961 gccgggctat cggagaacga gttggcagaa gtgctggggg gcaacgccgc ccggctgttg
     1021 cgcctggaat cgccggagcc gctgtagcgg cagccggcgg gccgggcttg tccgcgaggt
     1081 gcactgtggc gctgctcggg cagctggaag agtttcatct gcagcacatc gtggcgctgc
     1141 tgcaggtgga acggcagacc ggggaactga tgctggaaca ggacgggcaa cgggcgtgcc
     1201 tctatttcca ggatggggcc atcgtccacg cggtcgccgg caaggaaacc ggctacgagg
     1261 ccgcgctggc gccgttcctc tggaccagcg gcaagttcca cttcgaggcc tatgagcccg
     1321 aggtcgagcc gaccatcacg gccggcaatg cggccattgt cgcggcgggc cgccggctgg
     1381 ccgtggaagc cgaggaagtg cgccgctcgg tcacgagcat gaacatgctt gtccacctcg
     1441 tgcctcaggt cgatacgccc gccgggcaga tcaatttgag cttcagcgag tggcgcttcc
     1501 tgactctggt cgatgggcgg cgcgacctgg ccgccatcgg cgcgcaattg aagcaggcgg
     1561 acttcgccgt gcggctgatc gccaaccggc tggtcaagaa ccacctgatc gacctgcgcg
     1621 accctcgcca gacgatgctg cgcatgacgg ccatgccgac cagcctcgat ctccgcccgc
     1681 ccgccgaccc gctgctggcg ctcatggacg atttgacgct cgatatgctg ctcgccaacc
     1741 accgcgatcg ggtggcgcgc gcgcgggcgc gcgtgcaggt gctgaccagc gacgatcgct
     1801 cgctggtgct ggccgtggag ggccggcccg atctggccga ccggctgctt ctctccgagg
     1861 tggtgctggc ccgcctcggc gtgccgcgca atgcgaccgt gcatgtgcgg ctgctcgatg
     1921 atcgcgcata acttggcaaa cgcgtgagca ataccggctc accacagggg tgacgcctgg
     1981 gcattgcggg tacggtgttc tcgcgcacgg tgaggtaacc tggagttggg gatgacggcg
     2041 ccggcaattt agcccgattc gcccgaaatc ctgctacctt tccgcgcgct cttcgttgat
     2101 tatgacgacg gagggtgcga cagtggcagc agtccttttc ctacaaacac caattctcta
     2161 tggccgaagg accgagcggc cgcccatgag cgacatgggc gccgaccccg tggcatacga
     2221 gtggctgcgg ctcggcgtgc gcgccgcgct cgaagataac cgcgaggatg cccgctatta
     2281 tttcgtccag gccgtgcgca cggacctgaa cttcgcccgt gcctggctct acctgggcgg
     2341 cgtggccgac gacccggcgc tgacgctgtc gtgcctgcaa aaggtgctgc ggctcgaccc
     2401 cagcgagacc caggcgcgcg ccgggctggt ttgggcgcgc gagaagctgg ggctgttgca
     2461 gccgctgttg ccgctgctgt cctcgatgtc gcaggccgat ctgctgccgc aacccgcgcc
     2521 cgcggccacg gtccaagcgc ccgccgcccc gccggcgccc ccgcgcaccc tgctctgggg
     2581 cgggctgccc gcgcccaccc tgccctccat gcccgccatc gccaccccac cgcccgccgc
     2641 gcaagcggcc ccggcgcccc gtgccgtgcc gcctcccgcc gcgcccgaag ccccgcccca
     2701 ggaagaggaa gacagcgtgg cccgcctgct gcgccggggc gtgcgcgctg ccgtcgaggg
     2761 caaccgcggg cgcgcccgct actatttcgt gaaggccatc gccgtcgatc ccggtaacac
     2821 gcgcgcctgg ctctacttcg ggggtgtggc cgaagacccc tcgaccaccc tggcctgcat
     2881 ggaacgcgta ctgcgcgacg agcccgacaa cgcccaggcg cgcgcgggcg cggactgggc
     2941 cgtgcgccgg ctcggcatca acgcacc
//
LOCUS       DASUYC010001041         5027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_968691,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001041 DASUYC010000000
VERSION     DASUYC010001041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5027
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968691"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..244)
                     /locus_tag="VF276_17010"
     CDS             complement(71..244)
                     /locus_tag="VF276_17010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608604.1"
                     /translation="MADNRNWDAAGDVKAGANYTVWRTARWTPREFAALAQEEQFKYL
                     ALCAHLAPSSHNT"
     gene            complement(293..1252)
                     /locus_tag="VF276_17015"
     CDS             complement(293..1252)
                     /locus_tag="VF276_17015"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEX6608605.1"
                     /translation="MYAYYPASGDLVHYAPAAVHRLTQVTSNSRLLQDAPLEMSWPEI
                     RAVFDDLTGAVAGASVGNNIAHALAMDLRPRALKIADPNFFKVANANRVRLTYRDIVL
                     TNRRKAEERTPFGLKNKAIGAAEQIHALDPFMQVWAYYEGVTPDNVAGFVGGNRVEPR
                     AHYVIEETDNPDTKVFIRRAAREQRVHLIMATDLGSAVQVDVRPFDLDPAASPAVGAA
                     DAELYARQAAAQAQPGNREVFWHFVDSLIGVAYRERGEFGHLMAGRLPRIAASIPQLG
                     STATMAGGIIAEVVARIALGHRYPERFLIDKYTLELQTWGAWV"
     gene            complement(1296..2372)
                     /locus_tag="VF276_17020"
     CDS             complement(1296..2372)
                     /locus_tag="VF276_17020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608606.1"
                     /translation="MPTQETQPADRAGSSAVRPVAEVIELVREWVDRPARARPDFAGA
                     YLWGGITTLPAEAPFPLYRDVDVVVVLTQGAPEDEEEVCYRGLILEVIWQNLAAHQEV
                     AAVLADPSRGPNLATTQILADPTGILAPLHQAVAAGYGWRRWIQARCAAEKAAVAEWL
                     ATMRQAATPLDRLIAARVVLGMLSGLLAVAQLARPTTRRALALLGELLEAQERPDLHE
                     AALTLMGSAHLSRADVQALHDQTVSAFDRAVEVYQTPIPFGFALRAHVRPYHVEGTQE
                     MIDAGQYREALFWITCLDTAYRALENDAPTAETPVFAAQFHALLAALGYTSAEAWAER
                     VAAAERLALAIYRIADALAARYPD"
     gene            complement(2576..4510)
                     /locus_tag="VF276_17025"
     CDS             complement(2576..4510)
                     /locus_tag="VF276_17025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608607.1"
                     /translation="MSSLTSASPGATLGVPARPGLAVPATLRFNLLMAGLSLLMAGVF
                     VLNQWAASHNLATDADVLPWTLLLLGTGLLSGFVLGVVAFRGRAAGIPAWRSLPPGYG
                     LSLLGVLVFLGGALLDLLWRAAMLSYPLGDDLLRPTSLLTALGAVLIGTGPLRAVWQS
                     SGDRGERVPWRIILPALVAATELLITLTLFTQRANPLVDTAYVATQPPFRAPLNEIYR
                     MNADGTGQTRLLTRPGHFFWGPAWSPDGKRIAFTMGGEQDPGVLYIANPDGSQAVKLT
                     QDGRGSYLPAWSPDGRQIAFLSQAPRNGHFGEVAVINADGSGETRLTDNGGWNYGVSW
                     SPDGQRLVFGSRQSGTWQLYTTAADGTDQLLLDTGGGGNAPAWSPDGRRIAFTSDRTG
                     QDHIYVVAVSDAHVQRLTSGESHNDNAVWSPDGLQIAFTSDRAGNAEIYVMQADGSGV
                     RNLTASPGLDSLVPGWSPDGSQILYTAVVHAPPSDPFDRQSLGIAAILIQSALLIGVL
                     LFVARRWRLPAGALALVIVLSSLAALALQDHYELLPGVILTALLAEILYALMLRQQEG
                     TLAARPPRFRLFATLVPALFYALYLVTVLVTQGLAWTADIAAGVVLLAGATGLLISFL
                     LLPLGDRRAAIPRPAPDSER"
BASE COUNT          850 a         1683 c         1699 g          795 t
ORIGIN      
        1 cgtttgcccg aacgtgccgc gatccaaata cagctggagg gtggcctcct gcggatccag
       61 tttgacacgg ttaggtgtta tggctgctag gcgccagatg ggcgcacagc gccaaatact
      121 tgaactgctc ctcctgcgct aaggcggcga actcgcgggg ggtccaacgc gccgtacgcc
      181 acaccgtata gttcgcgccc gctttgacgt cgcctgcggc gtcccaattg cggttatctg
      241 ccattcgcac cgatccttcc cctcgcctca ctcccccgtg gtttggtggc ccttacaccc
      301 aggcccccca cgtttggagt tccagcgtat acttgtcgat gaggaaccgc tccggatatc
      361 ggtggcccag cgctatgcgg gccacgacct cggcgattat ccccccggcc atggtcgccg
      421 tggagccgag ttgcgggata ctcgccgcga tccggggtag gcggccggcc atcaggtgcc
      481 cgaactcccc acgctcgcgg taggccactc caatcaaact gtccacgaag tgccagaaca
      541 cctcccgatt gccgggttgc gcctgcgctg ccgcttgccg cgcgtataac tccgcatccg
      601 ccgcgccgac cgccggcgag gccgcgggat ccaggtcgaa gggccggaca tccacctgca
      661 ccgccgaccc caggtccgtc gccataatca ggtgcacgcg ctgttcgcgg gcggcgcggc
      721 ggatgaagac tttggtgtcc gggttgtcgg tttcctcgat cacatagtgc gcccgcggct
      781 ccacgcggtt gccgcccaca aagccggcga cgttgtccgg cgtcaccccc tcgtagtacg
      841 cccacacctg catgaacggg tcgagggcgt ggatctgctc ggcggcgcca atggctttgt
      901 tcttgaggcc aaacggcgtt cgttcctccg ctttccggcg gttcgttaac acgatgtccc
      961 ggtaggttag gcgcacgcga ttggcgttgg cgactttgaa gaagttggga tcggcgattt
     1021 tcagcgcgcg gggccgcaga tccatggcca gcgcatgggc gatgttatta cccacgctcg
     1081 cccccgccac ggcccccgtg aggtcatcaa agacggcccg gatctcgggc caactcattt
     1141 cgagtggcgc gtcctgcagc aagcgggagt tactggtcac ctgcgtgagg cgatggactg
     1201 cggccggcgc atagtggacg agatcgccgg acgcgggata atacgcatac atgccataat
     1261 cacgctgtgc tggggatccg cggcgcagat tgtcgttaat caggataacg cgccgccagg
     1321 gcgtcggcga tgcggtagat cgccagggcc aaccgctcag cggcggccac gcgctcggcc
     1381 cacgcctcgg ctgaggtata ccccagcgcc gccagcaggg cgtggaactg cgccgcgaac
     1441 accggtgtct ctgccgtggg cgcatcattc tccagtgcgc gataggccgt gtcgaggcag
     1501 gtgatccaga acagggcttc ccgatactgg ccggcatcga tcatctcctg cgtaccttcg
     1561 acatgatacg ggcgcacatg ggcgcggagg gcgaagccaa acggaatcgg cgtctggtag
     1621 acttcgacgg cgcgatcgaa ggcgctcacg gtctggtcat gcagcgcctg cacatccgcc
     1681 cggctcaagt gcgccgatcc catcagggtg agcgccgcct cgtgcagatc cggccgctct
     1741 tgtgcttcga gtagctcgcc cagcagggcg agcgcgcgcc gggtggtcgg ccgcgctagt
     1801 tgcgcgacgg ccagcaggcc actgagcatc cccagaacga cccgcgccgc aatcagacga
     1861 tcgagcggtg tcgcggcctg acgcatggtg gccagccatt ccgccaccgc tgccttttcc
     1921 gcggcgcagc gggcctggat ccatcgccgc cagccatagc cggcggcgac tgcttggtgc
     1981 aaaggggcga ggatgccggt cgggtcggcc agaatctgcg ttgtcgccag gttcggaccc
     2041 cggctggggt cggccagcac ggcggccact tcctggtggg cggcgaggtt ctgccagatg
     2101 acctccagga tgagaccccg gtagcacacc tcctcttcgt cctcgggtgc gccctgggtt
     2161 aagacgacga cgacgtcgac atcccggtac aggggaaatg gcgcctccgc cggcaacgta
     2221 gtgatcccgc cccacagata cgcgccggca aaatcgggcc gggcgcgggc ggggcggtcc
     2281 acccactcgc gcacaagttc gatcacttcg gcaaccggcc ggacggcgct actgcccgcc
     2341 cgatcagccg gttgggtttc ctgcgtcggc atggaaatgg ctccaatgtt ggggcttttg
     2401 gttgagcgta gcatcactat gccgagtagc agaaatacga actgggccct cggctcctct
     2461 aacgcccctt ggcggtagat ggttgccata ccaggcagta gcgcggcccc agggccgcgc
     2521 tgagcgggat cgcacgcagt ttcccgtacc gtcacccatg tcccgtcagt ccgcgtcatc
     2581 gctcagaatc gggcgcaggc cgggggatgg cggccctcct gtcacccagg ggcagcagca
     2641 ggaagctgat cagcaggccc gtggcgccgg cgagcaagac cacgcccgcc gcgatatccg
     2701 ccgtccaggc cagcccttgc gtgaccagca ccgtcaccag gtagagcgcg tagaataggg
     2761 ccggcacgag cgtcgcgaac aagcggaacc ggggcggccg cgcggccagc gtcccttcct
     2821 gctggcgcag catgagcgca tagaggatct cggccagcag ggccgtcagg atgactcccg
     2881 gcaggagttc atagtggtcc tgcagcgcca gtgcggcgag gctgctcagc acgatcacca
     2941 gggcgagggc cccggccggt aggcgccagc gacgggcgac gaacagcagg accccgatca
     3001 gcaacgcact ctggatcagg atggccgcga tccccaggga ctggcgatcg aacgggtcac
     3061 tcgggggcgc atgaaccaca gccgtataca ggatctggga cccatcgggc gaccagccgg
     3121 gcacgaggct gtccaggccc gggctggcgg tgaggttccg gactccgcta ccgtccgcct
     3181 gcatcacata gatctccgcg ttgcccgccc ggtcggacgt gaaggcgatc tgcagaccgt
     3241 ccggcgacca gacggcgttg tcgttgtgac tctcgccgct ggtcaggcgt tggacgtggg
     3301 cgtcacttac cgccacaacg tagatgtggt cctggccggt gcggtcggag gtaaaggcga
     3361 tccggcggcc gtccggggac caggccggcg cgttgccgcc cccgccggta tcgagcagca
     3421 gctgatccgt gccatccgcc gcggtggtgt agagctgcca agtcccgctt tgccgggagc
     3481 cgaacacgag gcgctggccg tcgggcgacc agctgacgcc gtagttccac ccgccgttgt
     3541 cggtcagtcg cgtttcgccg ctgccgtctg cgttgatgac cgcgacctcg ccgaagtggc
     3601 cgttgcgcgg ggcctgcgac aggaaggcaa tctggcgacc gtcgggcgac caggcgggca
     3661 ggtagctccc gcgcccgtcc tgtgtcaact tgaccgcctg gctgccatcg gggttggcga
     3721 tgtaaagcac ccccggatcc tgctcgccgc ccatcgtgaa ggcaatgcgc ttcccgtccg
     3781 gggaccacgc gggaccccag aagaagtggc cgggacgggt cagcagccgg gtttgcccgg
     3841 tgccgtcggc gttcatacgg tagatctcat tgagcggggc gcggaacggc ggttgcgtgg
     3901 cgacgtaggc cgtatcgacc aggggattcg cccgctgggt gaagagcgtc agcgtgatca
     3961 acagctcggt ggccgcgacc agcgccggca ggatgatccg ccagggtacg cgctcgcccc
     4021 ggtccccgct gctctgccag acggcgcgta gcggtcccgt gccgatgagc acggctccca
     4081 gcgcggttag gagactcgtg ggtctgagca gatcgtcgcc gagtgggtac gagagcattg
     4141 ctgctcgcca gagaaggtcg agcaaagcgc cgccgaggaa gaccagcacg ccgagcaggg
     4201 ataggccata gccgggcggg agggaccgcc aggccgggat accagcggcc cgcccgcgga
     4261 acgcaacgac accgagcacg aagcccgaga gcagccccgt gccgaggagg agcagcgtcc
     4321 acggcagcac gtcagcatcc gtcgctagat tgtggctagc agcccactgg ttcagcacaa
     4381 agaccccggc catgagtagg gacaaaccgg ccatcaacag gttaaagcgg agtgtggccg
     4441 gcacggcgag ccccggcctt gcgggcacgc cgagcgtggc gcccggcgag gctgaagtga
     4501 gggaactcat tagctgtcct cctgacgtgc ggtggaccgc gtcacacctg cggcgcggga
     4561 agggtccaac aagcaaagac atggaccgcc cggtgccgcg ccaagacgcg gcgccggtca
     4621 ctggtcttgg gcatgccccc aacgaagcac accaggagca aagaatcagc cggatgactc
     4681 ctcaccgaga cgtgggcctg ctacggcgcg gtggtcgccc atccgcgccg taggggcgtc
     4741 ggagcgcctg agctgccctc gctcgtcggc acgccgctgt cgagcggtga acatggctaa
     4801 tcggggtccg gcacggtgcg ctggcgcaac ccccgcccca gcgtcaggag gagtagcccc
     4861 aggccaccca ctcgcacaat ggcaatgact tcggccaccg gtaggacagc gcaccgccgc
     4921 gcctgatcgt ccggttgtgt ttcctgcgtc ggcactgacc tgtctccagt agggggtcac
     4981 acggataagc gtggattcat agcgtatcat gccgggccgc ccgccgg
//
LOCUS       DASUYC010001042         6365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_2024592, whole genome shotgun sequence.
ACCESSION   DASUYC010001042 DASUYC010000000
VERSION     DASUYC010001042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6365
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2024592"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..2684
                     /gene="mgtA"
                     /locus_tag="VF276_17030"
     CDS             72..2684
                     /gene="mgtA"
                     /locus_tag="VF276_17030"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HEX6608608.1"
                     /translation="MGTTVQERREPGAALPPAAGPGPVPLDLPSLYQRLNSSPAGLSS
                     AAAARRLVAGGANDPGARAQRGWPAQVLGAFGSPLNAILLIAAGVSAVLGEPVNAAII
                     ATMVVVSGILNFAQTYRSQQAADRLRQEVAPTATVLRDGAWGELPRRAVVAGDVIRLT
                     AGDLVPADGRLIESRDLHVQEAALTGESLPAEKEAADPPGVPADPSGARYRVFLGTSV
                     VSGTATALITATGGATAFGEIAARLAARPPETEFERGTRQFGLLITRTVFFLVVFIFL
                     AAAALHRDPLESLLFGVALAVGLTPEFLPMIISITLSQGALHMARRKVIVKNLAAIQN
                     FGSIDILCSDKTGTLTSGEMRLDRHLDPLGAPSERVFLLGYLNSYYDTGVPTPSQAAM
                     LRPAAGNPLDTAILQHEHPDLAAYQKLDEVPFDFERRCLSVIVAWPGGTRLLVAKGAP
                     EQLVARCTAYEVAGVAHPLDAAARAPIESVYGDLHAAGLRVLAVAYRQVPVQPVYRIA
                     DETDLVLAGFLSFVDAPLADAGAAVAALRADGVEVKIITGDNELVARHVCAQVGLPTD
                     RLVLGDALDRIGDAALGRVAEQTSIFARVSPAQKNRIILALKSRGHVVGYMGDGINDA
                     PSLHAADVGISVAGAVDVAKDAAAIILLERGLDILHSGILEGRKAFGNVMKYLLMGTS
                     SNFGNMFSMAGAFLFLPFLPLLPTQILLNNFLYDLAQVTIPTDTVDPGYIHKPHRWDI
                     GLLRDFMLFIGPISSLYDFLTFGALIWMFQAGEPLFHTGWFVESLATQTLVLFVIRTA
                     GNPLRSRPSRALTLTTLGVVLLALILPYLPGADLLGFVPLPLTFYPFLAAVVCTYLLL
                     VEVVKRRLLSRMLG"
     gene            3135..4148
                     /locus_tag="VF276_17035"
     CDS             3135..4148
                     /locus_tag="VF276_17035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6608609.1"
                     /translation="MPDRVTPPRVSAVITTYNYERYVAAAIESVLGQTVPPDEVVVVD
                     DGSTDGTAGVVARYAGRGVRYIRQANAGAGAARNRGIDETRGELIAFLDADDCWLPDK
                     LERQLAHLRRYPAVGLVTGDEWQVFATDRPAVRERRRPARAARLYPRILIENLVGNPS
                     LVLVRRACFTQVGEFDETLPLGQDWEMWIRIARAYPIGVIDAPLIEFLQHDGSLTAGK
                     AAERHRSNQTIRRRYLGAVRSPARRAYLWCAGQSMSYFYMGAALADQGAARGATLRAA
                     LPALALDPSYRTKEKVGLVVRAVLGPGGFARIRAALHRVARPAPVADFAPRRREGYED
                     GLD"
     gene            complement(4234..6270)
                     /locus_tag="VF276_17040"
     CDS             complement(4234..6270)
                     /locus_tag="VF276_17040"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HEX6608610.1"
                     /translation="MNITRVRLGLAVLVMLALLAGLGRSALAVEVPPLGSLIYLQTNG
                     PTDDISDGDWYGSNQNGAVPGYHYFSVTVPCGWPSNLTIYFDLQSPEMNTNPDAQLYD
                     ELYTTYQPTEFELYPPSVTVRPGPTTPNVPAPGTGIPGSRITYPPANVPVTTGGISTV
                     GPAEGWVRYHSLPAPVTCGQYLLRSQANGDNQNGWRLRVGSDNDNDPNNAPPPNYDNP
                     DGLPGTGDELALGVLQGAYQHDQTTVATQCLDLYEFVAPGAATVTFHNFDMDLSLDPS
                     RNNVTYYDPSGTAHTGTTSDQNVWNNGTQTSRGAGDVIANPQPGWWRIHSCTGEHNEF
                     IQEGQFSIPAYYTQPPYPVMTLTKDDGRTVVGPGDVLTYTINFANTSNTTNHPGAAVN
                     VVLTDNLPANATYVSCGFNAPTTGTCSQAAGVVTWNVSGIIAAGAGGSVRLTLQVNAN
                     ATGTVVNNVTVNYGDPLGNIYPPVPAGDTDTIQQVAPTPTATPTPPAGPTATPTVTVT
                     PGGPTATPTQPAPPTATPPAPPPPTATPPRPGKTPPPRPTPRPPTATPTGTTTPTPPP
                     APEPPTNTPEPPTATPTATVEPPTATPTTAPAPPTNTPEPPTATPPAPPPATAAPAPP
                     SPTPSGNVGGGIGMPRSGAGAGDALGGWLALLLVALGLIGGGVLVLRRTRPARR"
BASE COUNT          842 a         2068 c         2386 g         1069 t
ORIGIN      
        1 tccccaccgg attgctgctc gccaggattc ctgggggtgg ataggctacc gccaggggaa
       61 ggggataggc catgggcacg acagtccaag agcgccggga gcccggcgcg gccctgccgc
      121 cggcagccgg gcccggcccg gtgcccctgg acctgccgag cctgtaccag cgcctgaaca
      181 gttccccggc cgggctgagc agcgccgcag cggcccgccg cctggtcgcc ggcggagcca
      241 acgatccggg cgcgcgggcg cagcgggggt ggccggccca ggtgctgggc gccttcggca
      301 gcccgctcaa cgccattttg ctcatcgccg cgggtgtgtc ggcggtgctg ggcgagccgg
      361 tcaacgccgc catcatcgcg accatggtgg tcgtcagcgg catcctgaat ttcgcgcaga
      421 cctatcgctc gcagcaggcc gcggatcggc tgcgccagga ggttgcgccc acggccaccg
      481 tgctgcgcga cggcgcctgg ggggagctgc cgcggcgcgc agtggtggcg ggcgacgtga
      541 tccggctgac ggccggcgac ctggtcccgg ccgatggccg gctgatcgag tcgcgcgacc
      601 tccatgtgca ggaggcggcg ctcaccggcg agtcgctgcc cgcggagaag gaagccgccg
      661 acccgccggg cgtgcccgcc gacccgagcg gcgcgcgcta ccgcgtcttc ctgggcacgt
      721 cggtggtcag cggcacggca acggcgttga tcaccgcgac gggcggggcc acggctttcg
      781 gcgagatcgc cgcccggctg gccgcgcggc ctccggaaac cgagttcgag cggggcaccc
      841 ggcagtttgg cctgctgatc acgcgcactg tgttcttcct ggtggtgttt atcttcctgg
      901 ccgccgcggc cttgcaccgc gacccactgg agtcgttgct gttcggcgtg gcgctggcgg
      961 tgggcctcac gccggaattc ctgccgatga tcatctcgat cacgctgtcg caaggcgcct
     1021 tgcacatggc gcgccgcaaa gtgatcgtca agaacctggc cgccatccag aacttcggca
     1081 gcatcgacat cctgtgcagc gacaagaccg gaaccttgac cagcggcgag atgcgcctgg
     1141 atcgccatct cgacccactg ggcgcgccgt cggagcgggt cttcttgctg ggctacctca
     1201 acagctatta cgataccggg gtgccgacgc ccagccaggc ggcgatgctg cggcccgcgg
     1261 ccggcaaccc tctggacacc gcgatcttgc agcacgagca ccccgatctg gcggcctatc
     1321 agaagctgga cgaggtgccg ttcgacttcg agcgccgctg cctctcggtc atcgtcgcct
     1381 ggcccggtgg cacgcggctg ctcgtcgcca agggcgcgcc ggagcagttg gtcgcccgct
     1441 gcacggccta cgaggtggcc ggagtggccc acccgctgga tgccgccgcc cgtgccccga
     1501 tcgaatcggt ctatggcgac ctccatgccg cgggtctgcg cgtgctggct gtggcctatc
     1561 gccaggtgcc cgtccaaccg gtctaccgga tcgccgacga gacggacctg gtgctggcgg
     1621 ggttcctcag cttcgtcgac gcgccgctgg ccgatgccgg ggcggcggtg gccgcgctgc
     1681 gcgccgacgg cgtggaggtc aagatcatca ccggcgataa cgagttggtg gcccgccacg
     1741 tctgcgccca ggtgggcctg cccaccgacc ggctggtgct gggcgacgcg ctggatcgca
     1801 tcggcgacgc ggcgctgggg cgcgtggccg agcagacgag catttttgcc cgcgtgtctc
     1861 cggcgcagaa gaaccggatc atcctggcgc ttaagagccg gggccatgtg gtgggctata
     1921 tgggcgacgg catcaacgac gcgccctcgc tgcatgccgc cgacgtgggc atctcggtag
     1981 ccggcgcggt ggacgtggcg aaggatgccg cggcgatcat cttgctggag cgcggcctgg
     2041 atatcctgca cagcgggatc ctggaagggc gcaaggcctt cggcaacgtc atgaagtacc
     2101 tgctgatggg caccagttcc aacttcggca acatgtttag catggccggc gcctttctct
     2161 tcctgccctt cctgcccctg ctgcccacgc agatcctgct gaacaacttc ctgtatgacc
     2221 tggcccaagt gactatcccc accgacactg tggaccccgg ctacatccat aagccgcacc
     2281 gctgggacat cggcctgctg cgcgacttca tgctcttcat cggaccgatc agttcgctgt
     2341 atgacttcct gaccttcggc gcgctgatct ggatgttcca ggccggcgag ccgctgttcc
     2401 acaccggctg gttcgtggag tcgctggcga cccagaccct ggtgttgttc gtgatccgca
     2461 cggcgggcaa cccgctgcgc agccgtccca gccgggcgct cactctgact acgctcggcg
     2521 tcgtgctgct ggcgctgatc ctgccctacc tgcccggcgc cgatctgctg ggcttcgtgc
     2581 cgctgcccct caccttctac ccgttcctgg ccgccgtggt ctgcacttac ctgctactgg
     2641 tcgaggtggt caaacggcgg ctgctcagcc ggatgctggg gtgaggtgac ctctctccgg
     2701 cccctgccct gcgcggggag gaggcggatt gtcgcgtgct tctccctttc cgccgcggga
     2761 aagggggggt caggttagtc gcgtacccgt atgcccagtt ccgccgccgg cgcgggcgga
     2821 ggtcggggga agttgcgaga ttgctaccat ttgcggggct ttgctcattg gattttaaca
     2881 ttcagccgat ataataaggc tgtacgtacg tcgcgggcgt ggcgccgggc tgtggtatac
     2941 tgcagccgac gggccggacg gcctgccgcg ggtggagcgc gaccggggca ggccgggcag
     3001 gggttggctg ccgggccgcc gagctgtgga tatgattcga ccggcgcaca gccgcgcggg
     3061 gaacgggcgc cggcgcatcg cacaccggcg cgccctccct cttatagact cagcccactg
     3121 ggaccttgac ctccatgcca gaccgggtta caccgccgcg cgtgagtgcg gtcatcacta
     3181 cctataacta tgagcggtac gtcgccgcgg cgattgaaag tgtattgggg caaacggtgc
     3241 ccccggacga ggtcgtggtg gtggacgacg gctcgaccga cggcaccgcc ggggtggtgg
     3301 cccgctatgc cgggcggggg gtgcgctata tccgccaggc caacgcgggg gccggcgcgg
     3361 cgcgcaaccg cggcatcgac gagacgcgcg gcgagttgat tgccttcctc gacgccgacg
     3421 actgctggct gcccgataag ctggagcgcc agctcgccca cctgcgccgc tatcccgcgg
     3481 tggggctggt cacgggcgac gagtggcagg tgttcgcgac ggaccggccc gcggtgcgcg
     3541 agcgccgccg cccggcccgc gccgcccggc tgtacccgcg catcttgatc gagaacctcg
     3601 tgggcaaccc ctcgctggtg ctggtgcggc gggcctgctt cacacaggtg ggggagttcg
     3661 acgagaccct gccgctgggc caggactggg agatgtggat ccgcatcgcc cgcgcctatc
     3721 ccatcggcgt gatcgacgcg ccgctcatcg aattcctgca gcacgacggc agcctcacgg
     3781 cggggaaggc cgccgaacgg caccggtcga accagaccat ccgccgccgt tacctgggcg
     3841 cggtgcggtc gccggcgcgg cgggcgtacc tgtggtgcgc ggggcagagc atgagctatt
     3901 tttatatggg cgcggcgctg gccgatcagg gggcggcgcg gggggcaacg ttgcgcgcgg
     3961 ccctgccggc cctggcgctg gatcccagct accggacaaa ggagaaggtg gggctggtcg
     4021 tgcgggcggt cctcggccca ggggggttcg cgcgcatccg ggccgccctg caccgcgtgg
     4081 cccgcccggc cccggttgcg gattttgcac cgcgaagacg cgaaggatac gaagatggtt
     4141 tagattagtc gtcttcttcg cgcgctacgc gccttcacgg tgaactcatg agttggtgcg
     4201 cccgccggtc agacgaccgg cgggcgcggt ggtttagcgg cgggcggggc gcgtccggcg
     4261 caggacgagc acgccgccac cgatcaggcc aagggcgacc agcagcaggg ccagccatcc
     4321 gccgagggca tccccggcgc cggcgccgct acgcggcatg ccgatgccgc cgccgacatt
     4381 gccgcttggc gtggggctgg gcggggcggg cgcggccgtc gcgggcggcg gcgcgggcgg
     4441 cgttgccgta ggcggctcgg gcgtattggt gggcggggcc ggggccgtcg tgggagtggc
     4501 cgtgggcggt tctaccgtcg cggtcggcgt ggccgtgggc ggctcaggcg tattcgtcgg
     4561 cggttccggg gccggaggcg gcgtcggcgt cgtcgtgccc gtgggcgtcg cggtcggcgg
     4621 gcgcggggtg gggcgcggcg gcggcgtctt gccggggcgc ggcggcgtgg ccgtgggcgg
     4681 cggcggcgcc ggcggcgtgg ccgtgggcgg cgccggctgg gtcggggtgg ccgtggggcc
     4741 gccgggcgtc acggtcacgg tcggggtcgc cgtgggcccg gccggcgggg tcggcgtggc
     4801 cgtcggcgtg ggcgcgacct gctggatcgt gtcggtatcg ccggcgggca cgggcggata
     4861 gatgttgccc aggggatccc cgtagttgac cgtcacgttg ttgaccacgg tgccggtggc
     4921 gttggcgttc acctgcaggg tcaggcggac gctgccgccg gcgcccgcgg cgatgatgcc
     4981 ggacacgttc caggtgacga cgccggcggc ctgcgagcag gtgcctgtgg tcggcgcgtt
     5041 aaagccgcag ctcacatagg tggcattggc cggcaggttg tcggtcagca ccacgttgac
     5101 cgcggcgccg gggtggttgg tcgtgttgga cgtgttggcg aagttgatcg tgtaggtcag
     5161 cacgtcaccc ggccccacga ccgtccgccc gtcgtccttc gtcagcgtca tgaccgggta
     5221 gggcggctgg gtgtagtagg ccgggatgct gaactggcct tcctggatga actcgttgtg
     5281 ctcgccggtg cagctatgga tgcgccacca acctggctgc gggttggcga ttacatcgcc
     5341 ggcgccgcga ctggtctgcg tgccgttatt ccagacgttc tggtcggagg tggtgccggt
     5401 atgggccgtc cccgatgggt cgtagtacgt gacgttgttg cggctcgggt cgagggagag
     5461 gtccatgtcg aagttgtgga aggtgacggt ggcggcgccg ggggccacga actcatagag
     5521 gtcgaggcac tgcgtggcga ccgtcgtctg atcgtgctga taggcgccct ggagcacgcc
     5581 tagcgccagt tcatcgccgg tgccgggcag gccgtcgggg ttgtcgtagt tgggcggcgg
     5641 cgcgttgttc gggtcgttgt cgttgtccga gccgacgcgc aggcgccagc cgttttgatt
     5701 atcgccgttg gcctggctgc gaagcaggta ctggccgcac gtcaccgggg cggggagcga
     5761 atggtagcgc acccatccct cggcggggcc gacggtggag atgccgccgg tcgtcacggg
     5821 cacgttggcc ggcgggtagg tgatccggga gccggggatg ccggtgcccg gcgcgggcac
     5881 gttcggcgtg gtcgggccgg ggcgcacggt gacgctgggc gggtagagct caaactcggt
     5941 cggttgatac gtcgtgtaca gttcgtcgta gagttgcgcg tcggggttgg tgttcatctc
     6001 cgggctttgc agatcgaagt aaatcgtcag attgctcggc catccgcacg gcacggtgac
     6061 ggagaaataa tgatagccgg ggacggcgcc gttctggttg ctgccgtacc agtcgccatc
     6121 ggaaatatcg tcggtggggc cattggtctg gagataaatc aggctgccga gggggggcac
     6181 ctctaccgcc agggccgagc ggcccagccc ggcgagtagc gccaacatga ccaggaccgc
     6241 taggcccagt cgaacccgcg tgatattcaa ttcgttacct ccctgatgca ctaagccaac
     6301 catggagcga ttgaggcaag caagcacagc gaccccgtac ccggtccagg gccgatcggc
     6361 ggttg
//
LOCUS       DASUYC010001043         4316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_176612,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001043 DASUYC010000000
VERSION     DASUYC010001043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176612"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VF276_17045"
     CDS             <1..576
                     /locus_tag="VF276_17045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013323235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase"
                     /protein_id="HEX6608611.1"
                     /translation="KLMQWNDEKLGGKGFHDWVPFDHPQLGPVEIGGWDFKYVWQNAP
                     AEYLPELCDRHCRFTIAHALMAPRLGLKQVDVTHQGADVYRIVAVLENRGFLPTYTSK
                     KGQERNIVRPIRVTLALPEGGALVTGPAEQEVGQLEGRANKAFGGFRSPSPTDSQRKV
                     EWVVRAPAGSTVQITAQAERAGTVRADVPLE"
     gene            1207..1950
                     /locus_tag="VF276_17050"
     CDS             1207..1950
                     /locus_tag="VF276_17050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608612.1"
                     /translation="MQHLKLSELKTPAPRRERRQNLMLVPGPARSADPLATAARARVR
                     HALVRALQEFLDRAGFTELERAADPAVAALLGRSYWATRADLLQGLAVGADGATLLDL
                     LERGLLAGLHGALNRGYADLQALGADLDRLKYVRLPVERVRHEVAAARWAGDVRHWTA
                     PSTPADLDGAALAEAGAPVLLVKYTPDALEDEDAPAPRLLEARLLLPGVGLVATAADG
                     GMLSLDVAALTRFLCAAEVAVLARPALAR"
     gene            1978..3141
                     /locus_tag="VF276_17055"
     CDS             1978..3141
                     /locus_tag="VF276_17055"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX6608613.1"
                     /translation="MIQESIPFYNPNLSYQENYDQGPFGLFATEPPALVRPQAGAASE
                     PGGVAPERFLGVPLAVPFGIPAGPLLNAAYVAAAFRWGYDLCHYKTVRSRPWPAHPFP
                     NVLQVGPAAPLRDTGAAPPRLVARPFAGNGAVPASQVGGLSITNSFGMPAQPVVVWQA
                     DVGRALDAAGPGQALVVSVVGTADPGDGPAQFAADFAHTAALAAAAGAPILEANLSCP
                     NVGGHGLLCHDIAAARAVCAAIRECVPDRPLLVKLGSYPATGAGQDALAALVAALAPY
                     VAGFSAINAVPYPVVDAVGTQALPGAGRARAGICGAAIRDMGLDVVRRLAALRAESGG
                     AWGIIGVGGVMTPADYRAYRRAGADVVQAAAGPMWNPRLALEIAAETQVAGAV"
     gene            3322..>4316
                     /gene="pyrF"
                     /locus_tag="VF276_17060"
     CDS             3322..>4316
                     /gene="pyrF"
                     /locus_tag="VF276_17060"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX6608614.1"
                     /translation="MSFNARLDAAIASKGSLLCVGLDPIYGDLPADQRGDDVTAALVA
                     FNRRIAGATAAQAVAFKLQLKVYTAEGPAGYAALIETCRYLKTTYPDHLLILDAKYGD
                     VGHVVARCAHEAFDLCGADAVTAFAHPGREALAPLLARPDRGCFVVCRTSNPGAAEMQ
                     DVAVVGGESYFLYMARHIAATWNAHENCGLVAGATYPAEMRRIRAAAPDLPLLVPGVG
                     AQGGDLAGSMQAGVNAAGAGLLISVSRGVMQAADPAAAAAEYVAAMAAARSAPAPEPS
                     AAPPAELDALLPALFDAGIIKFEPITLKSGRVSPYYHNLRALVAHPDLLRRVAEA"
BASE COUNT          570 a         1503 c         1572 g          671 t
ORIGIN      
        1 aagctgatgc agtggaacga cgagaagctg ggcggcaagg gcttccacga ctgggtgccg
       61 ttcgaccacc cgcaactcgg cccggtggag atcggcggct gggatttcaa gtacgtctgg
      121 cagaatgcgc ccgccgaata cctgcccgag ctctgcgacc gccactgccg cttcactatc
      181 gcccacgccc tgatggcgcc gcgcctgggg ctgaaacagg tcgatgtgac gcaccagggc
      241 gccgatgtgt accgcatcgt cgccgtgctg gaaaaccgcg gcttcctgcc cacctacacc
      301 agcaagaagg gccaggaacg caacatcgtc cgccccatcc gggtcacgct ggcgctgccg
      361 gagggcggcg cgctcgtcac cgggcccgcc gagcaggagg tgggccaact cgaaggccgc
      421 gccaacaagg ctttcggcgg gttccgctcc ccctcgccga ccgacagcca gcgcaaggtg
      481 gagtgggtgg tgcgcgcccc ggcgggcagc acggtccaga tcaccgccca ggccgaacgc
      541 gcgggcaccg tgcgcgccga tgtgcccctc gaataggcac cagcgcgaga gtgctcccct
      601 gcgggacggc ggccacgccg tcccgccgcc tgttgtatat ccggctgttc tctatttgcc
      661 gccacttgtt taccggttgc aacctccact gcaacctggc gccgctatca tcttcctgcc
      721 ggcggcgcgt ggccggcacc gggccattgc cgggcggttt gtgacgctca gaccgcttga
      781 caagcccggc ggcctgtagt atcattgacc cggtattgag gcaaccttta agttggtccc
      841 gtgaggccga caaggtgccg gtaagcgtgc ggggcgcgat gcgaatcgtg gtgctcgccg
      901 ggagtttgcc gtggaccttg cgcgcctggg ggcggcaagg ttttttgttt gccctggagt
      961 ttgatcggat gtccgcactc gccgctaacc gcatagatcg cgccgccggc ctggccgggt
     1021 ggttggtgtc gctggcgggc agcggagccg ggcacggcca gcccgcgaat ccgggcgccg
     1081 gagtgatgcc cgattcacct gctggggccg ggcaaccggc cgatcacagc aaatatcgca
     1141 cgttggggga tggagtccgc gggacctgtc cccttttttc tgcctgacgc acggaggagg
     1201 caacccatgc agcacctgaa attgagcgag ttgaagaccc cggccccgcg tcgcgaacgc
     1261 cgccagaacc tgatgctggt gcccggtccg gcgcggtccg ccgatccgct ggcgacggcg
     1321 gcgcgagcgc gcgtgcgcca cgccctggtg cgcgccttgc aggagttcct cgaccgcgcc
     1381 ggtttcacgg agttggagcg cgccgccgac ccggcggtgg cggcgctgtt ggggcgcagc
     1441 tactgggcca cgcgagccga cctgctgcag gggctggccg tgggcgcaga cggcgcgacg
     1501 ctgctcgatc tgctggaacg gggcttgctg gccggcttgc atggcgcgct gaaccggggt
     1561 tacgccgacc tccaggcgct gggcgccgac ctggaccggc tcaagtatgt gcggctgccg
     1621 gtggagcggg tgcgccacga agtcgccgcc gcgcgctggg ccggcgatgt gcgccactgg
     1681 acggcgccca gcacgcccgc cgatctcgac ggcgcggcgc tggccgaggc cggagcgccg
     1741 gtgctgctgg tgaagtacac gcccgacgcg ctagaggacg aggatgcgcc cgcgccccgg
     1801 ctgctggaag cgcgcctgct gctgcctggc gtgggcctgg tggcgacggc agcggatggc
     1861 ggtatgttga gcctggacgt ggccgccctg acgcggttcc tctgtgcggc ggaggtggcc
     1921 gtgctggccc gcccggcgct ggcccgctag catccggcgc tatgaaatgg agtacgtgtg
     1981 atccaggagt cgatcccgtt ctataacccc aacctgagct accaggagaa ctacgaccag
     2041 ggcccgtttg gcctgttcgc cacggaaccg ccggccctgg tccgcccgca agccggcgcc
     2101 gcgtcggaac ctggtggcgt cgcacccgaa cgcttcctgg gtgtgccgct ggccgtgccc
     2161 ttcggcatcc cggcggggcc gctgctcaac gcggcgtatg tggcggcggc tttccgctgg
     2221 ggctacgacc tctgccacta caagacggtc cgcagccggc cctggcccgc gcacccgttc
     2281 cccaatgtgt tgcaggtagg gccggctgcg ccgctgcgcg acaccggcgc ggcgccgccc
     2341 cggctggtcg cgcgcccgtt cgccggaaac ggcgcggtgc ccgcgtccca ggtgggcggc
     2401 ttgagcatca ccaactcgtt cggcatgccg gcccagccgg tcgtcgtctg gcaggccgat
     2461 gtgggccggg cgctggatgc tgcggggccg ggtcaggcgc tggtcgtctc ggtagtcggc
     2521 acggcggacc ccggcgacgg ccccgcgcaa tttgccgccg atttcgcgca caccgcggcc
     2581 ctggccgccg cagccggggc gccgatcctg gaagccaacc tgtcctgccc gaacgtgggc
     2641 ggccacggcc tgctctgcca cgacatcgcg gcggcgcggg ccgtttgcgc ggccatccgc
     2701 gagtgtgtgc ccgaccggcc cctgctggtc aagctgggca gctacccggc caccggcgcc
     2761 gggcaagacg ccctggcggc cctggtggcg gccctggcgc cctacgtcgc cgggttctcg
     2821 gccatcaacg ccgtacccta cccggtggtg gacgctgtgg gcacccaggc tctgcccggc
     2881 gcggggcggg cgcgcgcagg tatctgcggc gcggccatcc gcgacatggg cctggacgtg
     2941 gtccggcggc tggcggcctt gcgcgcggag tccggcggcg cctggggcat tatcggggtg
     3001 ggcggcgtga tgacccccgc cgattaccgc gcctaccgcc gggcgggcgc cgacgtggtg
     3061 caggccgctg ccgggccgat gtggaatccg cgcctggccc tggagatcgc ggcggaaacg
     3121 caggtagccg gtgccgtttg agtgcctgcg cttgctctcg cccccgcgcc ctgctgtcac
     3181 ccagcggggc gttgtcgcgt tgtacgacag gaccgaattg gccgcttgcg gcaaagcgcg
     3241 ggccggtttg aaaccggccc ctacaagtga cggcatcgag gggttggccc actgacatga
     3301 agaaagaggt ttctcccgac catgagtttt aatgcacggc tggatgcggc gattgcgagc
     3361 aagggcagct tgctctgcgt gggcctggac ccgatctatg gcgatctgcc ggcggaccaa
     3421 cggggtgacg atgtgacggc ggcgctggtg gccttcaacc ggcgcatcgc cggcgccacg
     3481 gcggctcagg ccgtggcttt caagctgcaa ctgaaggtct acacggcgga ggggccggcg
     3541 ggctacgcgg cgctgatcga aacctgccgc tacctgaaga cgacctaccc cgaccacctg
     3601 ctgatcctgg acgccaagta cggcgacgtg ggccacgtgg tggcgcgctg cgcgcacgaa
     3661 gccttcgacc tctgcggtgc cgacgcggtg acggctttcg cccaccctgg ccgcgaggcg
     3721 ctggcaccgc tgctggcgcg gccggatcgt ggttgcttcg tcgtctgccg cacctcgaac
     3781 cctggcgccg ccgagatgca ggacgtggcg gtggtgggcg gcgagtccta cttcctctac
     3841 atggcgcgcc acatcgcggc gacttggaac gcgcacgaga actgcggcct ggtggccggg
     3901 gcgacctacc cggcggagat gcgccgcatc cgcgccgccg cgcccgatct gccgttgctg
     3961 gtgccgggcg tgggcgcgca gggcggcgac ctggccgggt cgatgcaggc cggcgtcaac
     4021 gcggcgggcg cgggcctgct gatctcggtg agccgcggcg tgatgcaggc ggctgacccg
     4081 gctgccgccg ccgccgaata cgtggctgct atggcggcgg cgcggtcagc gcccgccccg
     4141 gagccgtctg ccgcgccccc cgccgagctt gacgcgctgc tgccggccct gttcgatgcg
     4201 ggtatcatca agtttgagcc gatcacgctg aagtcgggcc gggtgtcgcc ctactatcac
     4261 aacctgcgcg cgctggtcgc gcaccccgac ttgctgcgcc gcgtggccga agcgta
//
LOCUS       DASUYC010001044         2789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1330730, whole genome shotgun sequence.
ACCESSION   DASUYC010001044 DASUYC010000000
VERSION     DASUYC010001044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1330730"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..2775
                     /locus_tag="VF276_17065"
     CDS             223..2775
                     /locus_tag="VF276_17065"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6608615.1"
                     /translation="MTERRYTCPVLIGRRREVEALERQWQLVAAGQGQAVLIAGDAGI
                     GKSRMLLEMAGRARRQGWRVLIGHCSPYDAHLPYAPLLDALRAYVHTLPLADVAALFG
                     PQAPELLKLLPELAALLPDAVPSPALAPAAEKRRLFEAVYRFLAGLTAERPLLFGLED
                     MHWADDTSLELLHLLARRISGDAILLFATYRSDEPVARPGTPWARSIADLERERLITE
                     VRLQPLDPDDIRAMVAEVFALDTPVGAEFVEAVYARTEGNPFFVEELLKALVESGELV
                     PAGPAWDRAPIDRLDLPRSVREAILRRLDGLDPLTLQVVDTAATVGRYFDFAVLQEVT
                     GLSEDALLIAVKQLLNQQLISLERRFGWDHQGYLFRHALTRDAIYERLLPQEQRALHR
                     RVAETLEQIYRQQGSTAGHLGELAHHYHSAGGWAQAHGYGVRAGDQARALDANAEAAG
                     HYTRALDAAARLGDVPAADYAQLHHTRARVYTLLGQLAPAEAGYTEARRLARRAGDSA
                     LELEILYDLSGLEAGHGNYGQALALGEEMLALAGSDAADPRALARALSRVGNVLDNLA
                     RLAGGMANHQRALALFTNLGDTWGIADSLDLIGMNNYLAGDAGAAHEYWARSLALFTE
                     LDDRERMASCQTSLGMTVTQFDGMCVQAASPEECLACTERGLALSRRIEWRAGEAYAL
                     ALTAYAQIGAGQYGAAWRLATEALALARSIDHPEWQVLSLTALALTAFDLGDLAGAGA
                     RLEQALDMARPAGARQCELRLAALLATVRAQQGDLPAAAALLDPMQPAIGRATNTWER
                     QATYAMALIEQARRHPARALDLVDSLLAGQAPAHLLVLKAQLLGALGRPAAG"
BASE COUNT          387 a          996 c         1001 g          405 t
ORIGIN      
        1 ccacagcgct ctccttctta tttttaccgc acgcgcgggg cgcatggatg gcggcggcgc
       61 cgggcgacac ctgtaaatgg ctcgtgacgc gactattata gagcattgac atgccgccgg
      121 ggatgcgcta aactgcggac acgaaggcgt tggtgcagtc ctccggcagc ccccgcgaca
      181 gagccggctc ttgttaagta ttgcctgacc acggttgaag ttatgaccga gcgacgctac
      241 acctgccccg tgctgattgg ccgccggcgc gaagtcgaag cgctggagcg gcagtggcag
      301 cttgtggccg cgggccaggg gcaggccgtc ctcatcgcgg gcgacgccgg catcggcaag
      361 agccgcatgc tgctggagat ggcgggccgc gcccgccgcc agggatggcg tgtgctgatc
      421 ggccattgct cgccctacga cgcacacctg ccctacgcgc ccctgctcga cgcgttgcgc
      481 gcctacgtcc acactctgcc cctcgccgac gtggcggcac tgttcggtcc ccaggcgccc
      541 gaactcttga agttgctgcc cgagctggcg gcgctgctgc ccgatgccgt gcccagcccc
      601 gcgctggcgc ccgccgccga gaagcggcgc ctctttgagg ccgtctatcg gttcctggcc
      661 ggcctgaccg ccgagcgccc gctgctgttc ggcctggaag acatgcactg ggccgacgat
      721 accagcctcg aattgctgca cctgctggcg cggcgcattt ccggcgacgc gatcttgctg
      781 ttcgccacct accgcagcga cgagccggtg gcccggcccg gcacgccctg ggcgcgcagc
      841 atcgccgatc tcgaacgcga gcgcctcatc accgaagtgc ggttgcagcc gctcgatcca
      901 gacgacatcc gcgccatggt ggccgaggtc tttgccctcg acacgccggt cggcgccgag
      961 tttgtcgagg ccgtctacgc tcgcaccgag ggcaatccct ttttcgtcga ggaattgctg
     1021 aaggcgctgg tggagtcggg cgagcttgtc cccgccggcc cggcctggga tcgcgcgccg
     1081 atcgaccggc tggatctgcc acgcagcgtg cgcgaggcga tcttgcgccg cctggacggc
     1141 ctcgatcccc tcacgttgca ggtggtcgat acggcggcca cggtcgggcg ctatttcgac
     1201 ttcgccgtct tgcaggaggt cacgggctta tccgaagacg ccttgctgat cgcggtcaag
     1261 cagttgctca accagcagtt gatctcgctg gagcgccgct tcggctggga ccaccagggc
     1321 tacctgttcc gccacgcgtt gacccgcgat gccatctatg agcggctgct gccgcaggag
     1381 cagcgggcgc tgcaccgccg ggtcgccgag acgctggagc agatctaccg gcagcagggc
     1441 agcaccgccg ggcacctggg cgaactggcc caccactacc actccgcggg cggctgggcg
     1501 caggcgcatg gctatggcgt ccgcgcgggc gaccaggcgc gggcgctcga cgccaacgcc
     1561 gaagccgccg ggcattatac gcgcgccctg gacgccgccg cgcggctggg cgacgtgccc
     1621 gccgccgatt acgcccaact gcaccacacg cgggcgcggg tctacacgct gctagggcag
     1681 ttggcccccg ccgaggccgg ctacaccgag gcccggcgcc tggcccggcg ggcgggcgac
     1741 tcggcgctcg aactggagat cctctacgac ctgtcggggc tggaggccgg gcacggcaac
     1801 tacggccagg cgctggcgct gggcgaggag atgctggcgc tggccggcag cgacgccgcc
     1861 gacccacgcg ccctggcacg cgccctgagc cgcgtgggca acgtgctcga caacctggcc
     1921 cggctggccg ggggcatggc gaaccaccag cgggcgctgg ccctgttcac caacctgggc
     1981 gatacctggg gcatcgccga cagcctcgac ctgatcggca tgaacaacta cctggcgggc
     2041 gacgccgggg ccgcgcacga gtactgggcg cgcagcctgg ccctgttcac cgagttggac
     2101 gaccgcgagc gcatggcgtc gtgccagacc agcctgggca tgacggtgac gcagttcgac
     2161 gggatgtgcg tgcaggccgc atcgcccgag gagtgcctgg cctgcaccga gcggggcctg
     2221 gcgctgagcc ggcgcatcga gtggcgggcg ggcgaagcct acgcgctggc cctgacggcc
     2281 tacgcccaga tcggcgcggg gcagtacggc gcggcgtggc ggctcgcgac cgaggcgctg
     2341 gccctggccc gatccatcga ccacccggaa tggcaggtgt tgagcctgac ggcgctagcc
     2401 ctgacggcgt tcgacctggg cgacctggcc ggggcgggcg cccggctgga gcaggcgctg
     2461 gacatggccc ggcccgccgg ggcgcgccag tgcgagttgc gtctcgccgc cctgctggcg
     2521 accgtgcgcg cgcaacaggg cgacctgcct gccgccgcgg cgctgctgga ccccatgcag
     2581 ccggccattg gccgggcgac gaacacctgg gagcggcagg cgacctacgc catggcgctc
     2641 atcgagcagg ctcgccgcca ccccgcgcgg gcgctggacc tggtggacag cctgctggcc
     2701 gggcaggcgc ccgcgcatct gctggtgctg aaagcgcagt tgctgggcgc gctgggtcgc
     2761 ccggcggcgg gctaagcgct gctggcgga
//
LOCUS       DASUYC010001045         2982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_2025697, whole genome shotgun sequence.
ACCESSION   DASUYC010001045 DASUYC010000000
VERSION     DASUYC010001045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2025697"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2369)
                     /locus_tag="VF276_17070"
     CDS             complement(<1..2369)
                     /locus_tag="VF276_17070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX6608616.1"
                     /translation="MTQMLELTPPSGVALAEPDAGPTLAGALTGLSAAEVLARRAQGQ
                     GNNVALATGRSYQEILRENVFTFINGVLFSIGAVLVALGRVSDAVISVGLVFINVIVS
                     LVQEVRAKRKLDRIALLTRPRATVIRDGRAQEIDPGEIVRGDLLVVHAGDQIVVDGQV
                     VGDGRMDVDESLLTGEADLIVKRAGDPVYSGSFCVTGGATYVAEKVGLAGLANQITLG
                     ARAFRRVYTPLQREINLVIRVLVLMASFFGILLVTAALVSDIPLVQSVQMAAVIAGLV
                     PNGLFMAITLAYAIGAVRIATRGALIQQANAVESLSNVDVLCLDKTGTLTANRINLHA
                     LYPIGLSEPELRGLLGDYAAGTRDGNRTNDAIVAATAGKPRQVREAVPFSSARKWSAL
                     AFDDPALRGVYVLGAPEMLAPAVRVDAEMEARMAAAAAEGMRVLLFAYRTDLVSLHDA
                     AEQPQLPTDLTPLGVLVFSDELRPEAKATLTGFAQAGIKLKIISGDNPHTVAALAQQA
                     GLGPDIRVVAGPDLAAMDDAQFAQVAEDTTVFGRITPQQKERLVQALRGRGHYVAMIG
                     DGVNDVLSLKQADLGIAMESGSQATRGVADIVLLQDSFAALPAAFQEGQRIRNGMLDI
                     MKLFMTRVLYMALLIVATGIVGPEFPFTPRQSSILVLLTVGIPTLALTVWAQPGAQPR
                     RRGLVRSLLGFVLPAMASLGLVAGGIYLGYFVMNLLALKAANPFVSDAAVFAQILPAA
                     RTALITVTVMCGLQLMLFAKPPSAAWVGGSPLSGDRRPAWLALALFGVY"
     gene            2721..>2982
                     /locus_tag="VF276_17075"
     CDS             2721..>2982
                     /locus_tag="VF276_17075"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX6608617.1"
                     /translation="MAETELHVGDMAPDFTLPAEDGRMVSLSDYRGKRVVLYFYPQDD
                     TPGCTTQACGFRDAYPQIEEQNAVVLGVSPDSEQSHRKFKTKY"
BASE COUNT          504 a         1017 c         1025 g          436 t
ORIGIN      
        1 cagtagacgc cgaacagggc cagggccagc caggccgggc ggcggtcgcc gctgagcggg
       61 ctgccgccca cccaggccgc gctcggcggc ttggcgaaca acatcaactg caacccgcac
      121 atcactgtca cggtgatcag cgcggtgcgc gccgcgggca agatctgcgc gaagacggcg
      181 gcgtcgctca cgaacgggtt ggccgccttc agcgccagca ggttcatcac gaagtagccc
      241 aggtagatgc ccccggcgac caggcccagc gaggccatgg cgggcagcac aaagccaagc
      301 agcgagcgca ccagcccgcg gcggcgcggt tgtgcaccgg gttgcgccca caccgtcagc
      361 gccagtgtcg ggatgcccac cgtcagcagc accaggatcg agctttgccg cggcgtgaag
      421 gggaactccg gcccgacgat gccggtggcg acgatcaaca gcgccatgta gagcacgcgc
      481 gtcatgaaca gcttcatgat gtcgagcatg ccgttgcgga tgcgctggcc ttcctggaac
      541 gcggcgggca acgcggcgaa cgagtcttgc agcagcacga tgtcggccac gccgcgggtc
      601 gcctggctgc cgctctccat ggcgatgccc aggtcggcct gcttgagcga gagcacgtcg
      661 ttgacgccgt cgccgatcat ggcgacatag tggccgcgcc cgcgcagcgc ctgcaccagc
      721 cgctctttct gctgcggagt gatgcggcca aagacggttg tgtcctcggc cacctgcgcg
      781 aactgcgcgt cgtccatggc ggccaggtcg gggccggcca ccacgcgaat gtcggggccg
      841 agccccgcct gctgcgccag cgccgccacg gtgtgcgggt tgtcgccgga gatgatcttc
      901 agcttgatgc ccgcctgggc aaagccggtc agcgtggcct tggcctccgg gcgcaactcg
      961 tcgctgaaga ccagcacgcc gagcggggtc agatcggtgg gcaactgcgg ctgctcggcg
     1021 gcgtcgtgca gcgacaccag gtcggtgcgg taggcgaaca gcagcacgcg catcccctcg
     1081 gcggcggcgg cggccatgcg cgcttccatc tcggcgtcca cgcgcacggc gggggccagc
     1141 atttcgggcg cacccagcac atagacaccg cgcagcgccg ggtcgtcaaa ggccagcgcg
     1201 ctccacttgc gcgccgagga gaagggcacc gcctcgcgca cctgccgggg cttgccggcg
     1261 gtagcggcta caatggcgtc gttggtgcgg ttgccgtcgc gggtgccggc ggcgtagtcg
     1321 cccagcaggc cgcgcaattc gggttcgctc aggccgattg ggtagagcgc gtggagattg
     1381 atgcggttag cggtcagcgt gccggtcttg tccaggcaga gcacatcgac attgctcagc
     1441 gactcgacgg cattggcctg ctggatcagc gcgccccgcg tggcgatgcg caccgcgccg
     1501 atggcgtagg ccagggtgat tgccatgaac aggccgttgg gcaccagtcc ggcgatcacg
     1561 gcggccatct gcacgctctg cacaagcggg atatcggaga ccagcgcggc ggtgacgagc
     1621 aagatgccga agaaggacgc catcaagacc agcacgcgga tgaccaggtt gatctcgcgc
     1681 tgcaagggcg tgtacacccg gcggaaagcg cgggcaccca gcgtgatctg gttggcgagg
     1741 ccggccagcc cgactttctc ggctacgtag gtcgcgccgc cggtcacgca gaagctgccg
     1801 gagtagaccg ggtcgcccgc gcgcttgacg atgagatcgg cttcgcccgt cagcagtgac
     1861 tcgtccacgt ccatgcgccc gtcgcccacc acctggccgt ccaccacgat ctggtctccg
     1921 gcgtggacca ccagtaggtc gccccgcacg atctcgccgg ggtcgatctc ctgcgcccgg
     1981 ccgtcgcgga tcacggtggc ccgcgggcgg gtcagcaggg cgatgcggtc gagtttgcgc
     2041 ttggcgcgta cctcttgcac caggctcacg atcacgttga tgaagaccag gcccacggag
     2101 atgacggcat cgctcacgcg acccagcgcc accagcaccg cgccgatgct gaacagcacg
     2161 ccgttgataa atgtgaagac gttctcgcgc aggatttctt gatacgagcg accggtcgcc
     2221 agggcgacat tgttgccctg gccctgcgca cggcgggcca ggacttcggc ggcggacagg
     2281 cccgtgagcg ccccggccag ggtagggccg gcatccggct ccgcgagcgc gacgccggac
     2341 ggcggggtca gttccagcat ctgggtcatc atatctccta tccgggttgc ccaggtgagc
     2401 ggtcggcacc cgcccacccg atctgttgta ggtgtaagtc tctgcccatt gtaaggccgc
     2461 gcccccgatc atacgagcgc cgcgccgccg atttcgagcg ggaccgctgc cgcatcttcc
     2521 cctgggacag ttgtcccgcc gggccggttt gaaaccggcc cctacagcgc cacgaggaac
     2581 tatcagggcg gcccggcacg tacactgaaa gagtattcgc gaccgggccg ccctgggatc
     2641 cgcggcaggg gctacccgga tagcccggcg acgcctgcca ccaaagaggc accggcccgc
     2701 cggaagagga ggctagttac atggccgaaa ccgaattgca tgtgggcgac atggcccccg
     2761 actttaccct accggcagag gacgggcgca tggtcagcct gtcggactat cgcggcaagc
     2821 gggtggtgct ctacttctac ccgcaggacg acacgcccgg ctgcaccacg caagcctgcg
     2881 gcttccgcga tgcctatccg cagatcgagg agcagaacgc cgtcgtgctg ggcgtcagcc
     2941 ccgacagcga acaatcgcat cgcaagttca agacgaagta cg
//
LOCUS       DASUYC010001046         4674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_408951,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001046 DASUYC010000000
VERSION     DASUYC010001046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4674
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408951"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VF276_17080"
     CDS             <1..517
                     /locus_tag="VF276_17080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608618.1"
                     /translation="ADGVLLWSEEQYNGPVIPPTSTLHLLKRDGSQANTVIATGEGTF
                     SGYSVSGDTVTWGFMPPNPDLRTYIYSISSRISRALTTGPALHPLVKDRTVVWTEGPF
                     GAAGTPPYWSIKAYNLDSGQVTTPVRDVFQMTQAWALARGNVVAFTIDSGPNHRGSDL
                     YALGFNFAGAP"
     gene            608..943
                     /locus_tag="VF276_17085"
     CDS             608..943
                     /locus_tag="VF276_17085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608619.1"
                     /translation="MRVSLLIAILGGVIGVASAIQALMTVGNQPDYQDRAVFGWAALI
                     LAITATGGVALARTRPGVAGIVMVVDGLLGLGAMLLFYINTWYAVAVPCWILAAALMF
                     TEGRRAAQP"
     gene            complement(965..3760)
                     /locus_tag="VF276_17090"
     CDS             complement(965..3760)
                     /locus_tag="VF276_17090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608620.1"
                     /translation="MFDIFAAGIVALVFLSLLGYALTGWLLPPALGGLRWATLPWTGY
                     AAFVVLAQFATQAGLNMAQTALLALILALAANGLYLWRGGAWPREIAAEHSRLGRLVA
                     GLTALVFLLGVLPLLFYGYHTIIGENWDGEIYLALGEYLRTYAQPALGQAPPNPLLHT
                     LLVPPYSGRTHGFSYFQAAVGALTGLPSLVTLAPVLALLRALAVPASYFFFRAGWGWT
                     PRPALAAAAVLGLNPFLLWITYNTFGMQVPSLGLLPLSAGAALLALRAPAQAERGATA
                     RLVAYAALVTAALSVTYHPALTAWVAIAGAGGLAVLLGSGRRWGRVLGAGLALGLGTV
                     ALSAVAQWLSLGGFLAQYAEQTAGLGLTSFTAPTDSLGLSLSFRSLLAAGPGQPLLAR
                     AVQAYGLLILVAGLICVALGALWLWGLVRERPSGALVQAATAGGGLVYMLLFLRPLDY
                     DYGWFKAQSFVAFVLVGAVVGGLTYLGRRLLPGRGLARDRELDTLKTNQYGAINRAAT
                     PYRVQAAVRWWRGVALAGALPALALLTTLGLLLWQYHWPLRYSAEMMDARQVYDYVRP
                     GDSALVSNSRLMPGRLFNGVLAYFLRDSRIYGTFRTANAAWDEVRPTGVYTWAVVPAH
                     EPPQLYGYRPANRVWGNALLSLYRAPGDLLYTQSWERKGDYPRSTEGAPLTWQVGVDA
                     VQPGAAAVPAGAGSSESRHLDLGLAVFAPQTLGLAVQPATGAPLSYTVQLAPGLQDLA
                     FDLPAPATLTLHPATDAGLLAVRWATLHESLPGGAPQPPVAAPPITDVLMLRITGQAQ
                     GDTVETTIGRVDTHAPDRQADRVVLDIYRSGGAGGEADHFGYWSFALTGDTATFRVPL
                     GGGSPQAGPGAQPLPDASRRGPAQTGRFTASLTFYHGATVLDTVTDIYTFEARPDSGG
                     ALAIDNIAVRALPLLFY"
     gene            3991..>4674
                     /locus_tag="VF276_17095"
     CDS             3991..>4674
                     /locus_tag="VF276_17095"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6608621.1"
                     /translation="MEFQLTEEQQFIRETARQFADEVLAPRAAHHDQTAEFTAEQARR
                     LGELGFLGMQVPEEYGGSALDSVSYVLALEEVSRGDAATSVTMTVQNSLVNWPLVRFG
                     TAEQKRQYLPRLATGEWLGCYALTEPVAGSDPASMGTGAVEDGDSYVLNGSKIFISNG
                     GVADLALVWAQTHRELKHKGVTAFIVEKGMPGFEVGAEEHKMGIRGSNTVELYFKNCR
                     VPKANILGPL"
BASE COUNT          820 a         1662 c         1576 g          616 t
ORIGIN      
        1 cgcggacggc gtgttgctct ggagcgagga gcaatataac ggcccggtga tcccaccgac
       61 atccacgctg cacctgctga agcgcgacgg cagccaggcc aataccgtca tcgccaccgg
      121 cgagggcacg ttcagcggct actccgtctc gggcgatacc gtcacctggg gcttcatgcc
      181 gcccaacccc gatctgcgca cctatatcta cagcatcagc agccgcatca gccgcgcgct
      241 gaccaccggc ccggcactgc acccgctggt caaggatcgc accgtggtct ggaccgaagg
      301 tccgtttggc gcggcgggca cgccgccgta ctggtcgatc aaagcctata acctggacag
      361 cgggcaggtc accaccccgg tgcgcgatgt gttccagatg acgcaagcct gggcgctggc
      421 gcggggcaac gtagtcgcct tcaccatcga cagcggcccc aaccaccgcg ggagcgacct
      481 ctacgcgctg ggcttcaatt tcgccggcgc gccctgaccc ggcggcttgc cctaccctgt
      541 ataatggctg cgggtaccct gcctgagcag gatacccgca gctagtttat tggggagagg
      601 accaccaatg cgcgttagtt tgctcatcgc catcctgggt ggcgtgatcg gggtcgcatc
      661 ggctatccag gcgcttatga cggtcggcaa ccaaccggat taccaagatc gcgccgtgtt
      721 cggctgggcc gcgttgatcc tggcaatcac cgcgaccggc ggcgtggcgc tagcccggac
      781 ccggcccggc gtggccggga tagtcatggt ggtcgatggg ctgctgggcc tgggcgccat
      841 gctgctgttc tacatcaaca cctggtatgc ggtcgcggtt ccttgctgga tccttgccgc
      901 cgcgctgatg ttcaccgagg gccgccgcgc agcccaaccc tgaacggcgc aggcatccgc
      961 ccggctaata gaacagcagc ggcagggcgc gcacggcgat gttgtcgatg gcaagcgcgc
     1021 caccggaatc gggccgcgcc tcgaaggtgt agatgtcggt cacggtgtcg agcacagtcg
     1081 cgccatggta aaacgtgagc gaggcggtga agcggccggt ctgcgcgggg ccgcggcggc
     1141 tggcgtcggg caagggttgc gcgcccggcc cggcttgcgg cgatcccccg cccagcggca
     1201 cgcggaacgt cgccgtgtcg ccggtcaggg cgaaggacca gtagccgaaa tggtcggcct
     1261 cgccccccgc gcccccgctg cggtaaatgt cgagcaccac ccggtcggcc tgcctgtcag
     1321 gcgcatgggt atcgacccgc ccgatggtcg tctccacggt gtcgccctgg gcctgcccgg
     1381 tgatgcgcaa catcagcacg tcggtgatgg gcggggcggc gacgggcggc tggggcgcgc
     1441 cgccgggcag cgactcatgc agcgtggccc agcgcacggc cagcaacccg gcatccgtcg
     1501 ccgggtgcaa ggtcagcgtc gcgggcgcgg gcaggtcgaa ggccaggtcc tgcaagcctg
     1561 gcgccagttg cacggtatac gagagcggcg cgcccgtcgc gggctgcacc gccaggccca
     1621 gcgtttgcgg cgcgaacacc gccaggccca ggtcgagatg gcgcgactcg ctgctgccgg
     1681 ccccggcggg cacggccgcc gcaccgggct gcaccgcgtc cacgcccacc tgccaggtga
     1741 gcggggcgcc ctcggtgctg cgcggatagt cgcccttgcg ctcccaggat tgggtgtaga
     1801 gcaggtcgcc cggcgcccgg tagaggctca gcagcgcgtt cccccagacg cggttggccg
     1861 ggcgatagcc gtaaagctgc ggcggctcgt gcgcgggcac caccgcccag gtatagacgc
     1921 ccgtgggccg cacctcgtcc caggcggcgt tggccgtgcg gaacgtgccg tagatgcggc
     1981 tgtcgcgcag gaaataggcc agcacgccgt tgaacagccg ccccggcatc aggcggctgt
     2041 tgctgaccag ggcgctatcg ccgggccgca cataatcgta cacctggcgc gcgtccatca
     2101 tctccgcgct gtagcgcagc ggccagtggt attgccacag cagcaagccc agcgtagtca
     2161 acaaggccag cgcgggcaag gccccagcca gggccacgcc ccgccaccat cgtaccgccg
     2221 cctggacccg atagggtgta gctgcgcgat ttatcgcgcc gtactggttg gtctttagcg
     2281 tgtccagttc ccgatcgcgg gccagcccgc gccccggcag gagccgccgc cccaggtagg
     2341 tcagcccgcc caccaccgcg cccaccagca cgaaggcaac gaacgactgc gctttgaacc
     2401 agccgtagtc atagtcgagc gggcgcagga acagcagcat gtagacgagg cccccgcccg
     2461 ctgtcgcggc ctgcaccagg gcgccggatg gccgctcgcg cacgaggccc cagagccaga
     2521 gcgcgcccag cgccacgcag atgagacccg cgaccaggat gagcaggcca tatgcctgca
     2581 cggcgcgggc gagcagcggc tggcccggcc ccgcggccag caggctgcga aacgacagcg
     2641 agagccccag gctgtcggtc ggcgcggtga aactggtcag gcccagcccc gccgtctgct
     2701 cggcatactg ggccaggaag ccgcccaggc tcaaccactg ggccaccgcg ctgagcgcga
     2761 ccgtgcccag acccagcgcc agcccggcgc ccagcacccg gccccagcgt cgtccgctgc
     2821 ccagcagcac cgccagcccg cccgccccgg cgatggcgac ccacgccgtg agcgccgggt
     2881 gataggtcac ggagagcgcc gccgtgacca gggccgcata ggccaccagc cgggcggtcg
     2941 cgccccgctc cgcctgcgcc ggggcgcgca gcgccagcag cgccgcgccc gcggagagcg
     3001 gcagcagccc caggctgggc acctgcatgc cgaaggtgtt ataggtaatc cagagcaaga
     3061 acgggttcag gccgagcacc gccgcggcag ccagggccgg gcgcggggtc cagccccacc
     3121 cggcccggaa aaagaaatag ctcgcgggca cggccagagc gcgcagcaag gccagcaccg
     3181 gcgccagcgt gaccaggctg ggcagcccgg tcagggcgcc caccgccgcc tggaagtagc
     3241 tgaagccgtg cgtccgcccg ctgtacggcg gcacgagcag cgtgtgcagc agcggattgg
     3301 gcggcgcctg ccccagcgcc ggttgcgcgt aggtgcgcag gtattcgccc agcgccaggt
     3361 agatctcgcc gtcccagttc tcgccgatga tggtgtgata gccgtagaac agcagcggca
     3421 gcacgcccag cagaaacacc agcgcggtca gcccggcgac cagccggccc agacggctgt
     3481 gctcggcggc tatctcgcgc ggccaggcgc cgccgcgcca caaatagagg ccgttggccg
     3541 ccagcgccag aatcagggcg agcagcgccg tctgcgccat gttcagcccg gcctgtgtgg
     3601 cgaactgggc cagcacgacg aacgccgcgt agccggtcca gggcaaggtc gcccagcgca
     3661 ggccgccgag tgcgggcggc agcaaccatc cggtgagcgc atagcccagc aaactgagga
     3721 agacgagcgc gacgatcccc gccgcgaaaa tatcgaacat tagaaaccct gcgtgcctgt
     3781 agcgtatgtt aagccgtaaa tcggcccatt ataccacacg gcaggagcca cacagccgta
     3841 tggtataatg gaggcgttga acagaccgcg aatcgcccgg tccaggagca caccacccat
     3901 ccggcgaagg caccacacca cgcagcacac aagacggcag cgatagcccc atccccccgc
     3961 tatcgccatg cagaaggagc tgaggaaaca atggagtttc aactcaccga agaacagcaa
     4021 ttcatccggg aaacggcccg ccagttcgct gacgaggtcc tggcgccacg cgccgcccac
     4081 catgatcaaa cggccgagtt caccgccgaa caggcccgcc gcctgggcga acttggcttc
     4141 ctcggcatgc aggtgcccga ggagtatggc ggctccgcgc tcgacagcgt cagttatgtg
     4201 ctggcactgg aagaagtcag ccggggcgac gccgccacgt ccgtcaccat gaccgtgcag
     4261 aattcgctgg tcaactggcc cctggtgcgc ttcggcaccg ccgagcagaa gcgccaatat
     4321 ctgccgcgcc tggcgaccgg cgagtggctc ggctgctacg cgctgaccga acccgtcgcc
     4381 ggatcggacc cggccagcat gggcaccggc gccgtcgagg acggcgacag ctatgtgctc
     4441 aacggatcca agatcttcat cagcaacggc ggcgtggccg atctcgccct cgtctgggcg
     4501 cagacccacc gcgagttgaa gcataagggc gtcacggcgt tcatcgtgga gaagggcatg
     4561 cccggcttcg aggtcggcgc cgaagagcat aagatgggca tccgcggctc gaacaccgtc
     4621 gaattgtact tcaagaactg ccgcgtgccc aaagccaata tcctcggccc cctc
//
LOCUS       DASUYC010001047         2326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_923181,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001047 DASUYC010000000
VERSION     DASUYC010001047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923181"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            913..1773
                     /locus_tag="VF276_17100"
     CDS             913..1773
                     /locus_tag="VF276_17100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608622.1"
                     /translation="MHDATAWRRDLGRRIGQAYAANPKVAAVMIAGSTGRGTADRYSD
                     LEVDIYWHAPPTDAERIAAATAAGGTNIALAEDPDEWEEQMDLGGFHAATSAFLVSTM
                     ERYLHAVLDEYSTDPSAQMRLYSVLHAQPLTGTDLTAGWQARAAAYPPPLRAAMLREN
                     LGFDGFGYAEDMFAARDDVLVLYDIFCGIGRQILGALLGLNGIYLPNPGFKSMNEIVD
                     EMRIAPPDLAARLRGAFRVPPPDGVRELHRLIAEVFTLVELHVPTFDLAPYRAKVGAR
                     RGVWDAPPAL"
     gene            1888..>2326
                     /locus_tag="VF276_17105"
     CDS             1888..>2326
                     /locus_tag="VF276_17105"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEX6608623.1"
                     /translation="MAQISEEDRQMLHTLLDAEPEAQARYNKLANYKREMSLDWIADA
                     PEPRLREQRIRRLVRRLWLTAFDPYYDLGGMTLSELDENYGPPRQLLRVPEAEQAEAL
                     RERRVLGAYHYRNFDDGGTSFYIVDADTKRVDEFMLVYRRPCVL"
BASE COUNT          397 a          837 c          724 g          368 t
ORIGIN      
        1 taaccccccc gcccccttcc cggcgcggga agggggagag cagggcggcg gcgatctccc
       61 catcctgcca cggggagaag ttatccgcgt acctaccacc ggcggctggg caaattaccc
      121 tcgcgaaata gcacttttgc gtgatgcccg gcggccccga tcccggctat cataaggctc
      181 aagcggcgtg cttcggtggc aggcgtggaa ctcgtcaccg gggtaggccg ccgcttttta
      241 tctaccgtct gatccgcgca atactcgcca ccacccggac tgggccggct tccccccggc
      301 ccgaagcccc cgcggctctc gccgccgccc ggtggcgccc cctgtgcccc ccgattcccc
      361 gtggttcccg gcccccggcc cgccgccgtc gccccggcgg cggcgagcca ccggcgggag
      421 tgctgagtgc tgagtgctga gaggttgtga aacaaacccc caggcacgaa atgggggagg
      481 gctgaaccac aagatgttgt ggttatgctg gcatactaac cactacctgt tgtggtcccg
      541 gtagcatagg gggtttgttt cacagcctct gagtgctgag gacgagtagc cagcggtcgg
      601 tggttagcta tcagccgtca gctatcagcc gtcagccgtc agctatcagc cgtcagtaaa
      661 gagagcatta ttactctgag tgcagcgaag agtctcggcc tactccagcc ttgtcactct
      721 gagcgcagcg aagagtctcg gcgcgtgcgc aaacaagacg acacgcatca ctgcgttcag
      781 cacgatgtca ggcgcagccc tctccccctt cccgcggcgg gaagggggcc ggggggttag
      841 gttggtcggg cgcgaacaag gtccccacgc gccgccaaaa cccgctataa tacccggcgg
      901 gagggacact acatgcacga cgcaactgcc tggcgccgcg atctgggccg gcgcatcggc
      961 caggcctatg ccgccaaccc caaggtcgcc gccgtcatga tcgccgggtc taccgggcgc
     1021 ggcaccgccg accgctactc cgacctcgaa gtcgatatct actggcacgc cccgcccacg
     1081 gacgccgagc gcatcgccgc cgcgaccgcc gcgggcggca ccaacatcgc cctggccgaa
     1141 gaccccgacg aatgggaaga gcagatggac ctgggcggct tccatgccgc caccagtgcc
     1201 ttcctggtca gcacaatgga acgctacctg cacgccgtcc tggacgagta cagcaccgac
     1261 cccagcgcgc agatgcgcct ctactccgtg ctccacgccc agccgctcac cggcacggac
     1321 ctcaccgccg ggtggcaggc gcgcgccgcg gcctacccgc cgcccctgcg cgccgccatg
     1381 ctgcgcgaaa acctcggctt cgacggcttt ggctacgccg aggacatgtt cgccgcccgc
     1441 gacgacgtgc tcgtgctcta cgacatcttc tgcggcattg gccgccagat cctgggcgcg
     1501 ctgctcggcc tcaacggcat ctacctgccc aaccccggct tcaagtcgat gaatgagatc
     1561 gtcgacgaga tgcgcatcgc cccgcccgac ctggctgccc gcctgcgcgg cgccttccgc
     1621 gtcccaccgc ccgacggcgt acgcgaactc caccgcctga tcgccgaggt cttcaccctg
     1681 gtcgaactcc acgtgcccac cttcgacctc gcgccctacc gggccaaagt cggggcccgc
     1741 cgcggcgtct gggacgcgcc gccggccctg tagtccccgg cgggtgtcac tctgagcgca
     1801 gcgcaggcca gcatcgcggc ctgtcactct gagcgcagcg aagagtctcg tcgccccacc
     1861 cctatcactc caaggaggtg accgaccatg gcccagataa gcgaagaaga tcgccagatg
     1921 ctgcacacgt tgctggacgc cgagccggag gcgcaggcgc ggtacaacaa actggcgaac
     1981 tacaagcggg agatgtccct ggactggatc gccgatgcgc cggagccgcg gctgcgcgag
     2041 cagcggattc gccggctggt ccggcgcctg tggctgaccg cgtttgatcc ttattatgat
     2101 ctcggcggta tgaccctcag cgaactggac gagaactacg ggccaccccg ccagttgctg
     2161 cgtgtgccgg aggcggagca agccgaggcg ctacgggagc gccgggtgct gggcgcgtat
     2221 cactaccgca acttcgatga cggcggcacc tcgttctata tcgtggatgc cgacacaaag
     2281 cgagttgacg agttcatgct cgtgtatcgg cggccctgtg tgctga
//
LOCUS       DASUYC010001048         2323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1564369, whole genome shotgun sequence.
ACCESSION   DASUYC010001048 DASUYC010000000
VERSION     DASUYC010001048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1564369"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="VF276_17110"
     CDS             complement(<1..950)
                     /locus_tag="VF276_17110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608624.1"
                     /translation="MRRSPWLYYSVLVVVVLALAGLGLALNAGLTPAGAAPVGPATGL
                     DMTSATHTSTGSCGFAWRTPPNPVLTTSHNVLRGVAARAANDVWAVGYGEAGRQSLIL
                     HWDGSAWSQVPSPNPSAGDNRLMAVAAISPDDAWAVGYGTVDALILHWDGSTWSQVNG
                     PGVPGSSVFLRGITAVAANDIWAVGYYGTDTGNSRTLTLHWNGTAWSIVPSPNAAGWA
                     NELYAVTALGTNNVWAVGAVTSVGAVTLHWDGFGWAVVTDPLSGNLTFPAFYGVAAHA
                     ANDIWAVGYQVGGTGYAGTLVVHWDGSTWSAVPSPNGGTG"
     gene            complement(1159..1971)
                     /locus_tag="VF276_17115"
     CDS             complement(1159..1971)
                     /locus_tag="VF276_17115"
                     /inference="COORDINATES: protein motif:HMM:NF024819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside adenylyltransferase
                     domain-containing protein"
                     /protein_id="HEX6608625.1"
                     /translation="MSGPVGNPTPYAAVNEVLGALLAGVQRILGPDLHGMYLYGSLAL
                     GDFTPHRSDIDAVVVTAGELTDAQTAALQALHSDLATRYVPWGSELEVTYMPQRALRR
                     YDPADARYPSIQRGERLVVEAQDSSGVVQRHVLREHGITLAGPPIREQIDPVPPDDLR
                     RAVLAMARGWLAGLRADPRPLRHQGYQGYVVMTICRMFYTLQFGTIVSKPAACRWARG
                     VLGRRWAPLIDRALAEDVGETVDMIAFALDRVRAYPEPGEPSPVDPQPPGPF"
BASE COUNT          384 a          849 c          730 g          360 t
ORIGIN      
        1 tggccggtgc cgccgttcgg gctgggcaca gcgctccagg tggagccgtc ccagtgcaca
       61 accagggtgc cggcgtagcc tgtgccgccg acctgatagc ccaccgccca gatgtcgttc
      121 gcggcgtggg ccgcgactcc atagaatgcg gggaaggtga gattgcccga cagcgggtcg
      181 gtcaccaccg cccaaccgaa gccatcccag tgcaaggtta ccgcccctac cgaggtcacg
      241 gcgccgacgg cccagacatt gttcgtgccg agtgccgtga cggcgtaaag ttcattcgcc
      301 cagcccgccg cgttcgggct gggcacgata ctccacgctg tgccgttcca atgcagcgtc
      361 agggtgcggc tgttgccggt gtcggttcca taatagccca ctgcccagat atcattggcc
      421 gccaccgcgg tgatgccgcg caggaagacg ctgctgccgg gcacgccggg gccgttgacc
      481 tggctccagg tgctgccgtc ccagtgcagg atgagcgcgt ccaccgtccc gtagccgacc
      541 gcccaggcat cgtccgggct gatcgccgcg acggccatca ggcggttatc gccggctgac
      601 ggattcgggc tgggcacttg gctccaggcg ctcccgtccc agtgcaggat caggctctgg
      661 cgcccggctt ctccgtaacc caccgcccag acatcgttcg ccgcacgggc cgccacgccg
      721 cgcaagacat tgtgagaggt agtgagaacc gggttgggcg gggtgcgcca ggcgaacccg
      781 caactaccgg tggatgtgtg ggtggcgctg gtcatatcca ggccggtcgc cgggccgacg
      841 ggggccgccc cggcgggcgt aaggccggca ttgagcgcca gtccgagacc ggctagcgcc
      901 agcacgacaa ccacgagcac agaataatat aaccaagggc tgcgacgcat ggtaggctcc
      961 taatatttat gaaacaggga acagtgatct ggggaacacc aggttgcggt actgatcagc
     1021 ctggtgctgc taaatcatac taaaagtcga tctgttttgc aatccctcgg cttggagccg
     1081 tagaatgccg tggccgttgt gcagccggtg aatatcgctg tccggccggt ccggttagtt
     1141 tggggatccg caccggactc agaatgggcc gggcggctgc gggtccacgg gactcggttc
     1201 ccccggctcg gggtacgccc gcacgcggtc cagcgcgaag gcgatcatgt ccaccgtctc
     1261 gcccacgtcc tcggccaggg cgcggtcgat cagcggcgcc cagcgccggc ccagcacgcc
     1321 ccgcgcccac cggcaggcgg ccggcttcga cacaatggtg ccgaactgta gcgtgtagaa
     1381 catgcggcag atcgtcatca cgacatagcc ctggtagccc tggtggcgca agggccgcgg
     1441 gtcggcgcgc aacccggcca gccacccccg cgccatcgcc agcaccgccc gccgcaggtc
     1501 gtcgggcggc accgggtcga tctgctcgcg gatgggcggc ccggccagcg tgatgccgtg
     1561 ctcgcgcagc acatggcgct ggacaacccc gctgctgtcc tgggcctcca cgaccagtcg
     1621 ctcgccgcgc tggatgctgg ggtagcgggc gtccgcaggg tcgtaccgcc gcagcgcgcg
     1681 ctgcggcata tacgtcacct ccaactccga gccccacggc acgtaccggg tcgccaggtc
     1741 gctgtgcaac gcctgcaacg ccgccgtctg cgcgtccgtc aactccccgg ctgtcaccac
     1801 gaccgcgtca atatcgctgc gatggggcgt gaaatcgccc agtgccagcg acccgtacag
     1861 gtacatgccg tgcaagtccg gccccaggat gcgctgcacc cccgccagca acgcgcccag
     1921 cacctcgttg actgccgcat aaggcgtcgg attccccacc ggtccgctca taccctcgac
     1981 ccccttccca caacctcgca tcgagtctac cacacccatc aacccccgcc cagcccggta
     2041 gcccggaggc tcaagcctcc ggctagcgct acaaagcccg cctgcgcggc ctgtcttccc
     2101 tatcccagcc cgcgcaggcg ggcttcgtag cgctagcgcg ctcgttcatg ggccgggcga
     2161 ggccgctccc gcgcgatgac cggctcccgc ccccgaagca cctgaccact gaccactgac
     2221 cactgaccac tgaccactga ccaccgacca ccgaccactg accaccgccc cctcgtcccc
     2281 aaccggcgtc ttatgctata ctgaggacgc catggttgat ttc
//
LOCUS       DASUYC010001049         3675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1103493, whole genome shotgun sequence.
ACCESSION   DASUYC010001049 DASUYC010000000
VERSION     DASUYC010001049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103493"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1985
                     /locus_tag="VF276_17120"
     CDS             <1..1985
                     /locus_tag="VF276_17120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608626.1"
                     /translation="HERQILRAADLNVEQAYLIGMRRRHNCGPHSWGIVRGLRLRLQA
                     GDLVLSPGVAFDLYGRELVVPAEVRVADAWLDRVGSPRVAVWLAYARVDLDIAPDGRP
                     DCVPGGYARVREEARLCFTVAPAGDEAQIDPRRPYQVPDTALSFGSQCEPPDDPAVRW
                     PVYLGQACRGDEKAPWCLVPEGDPGYLGEDGCGDDKGTPGADRQEIPEACKGITGPAR
                     PYAGLQGETVASASRVPVAATGADGPPERAPAARMQVGAEQDTGTRFAVEIADKQGAP
                     QERLTIDSAGPIALRGVARLSRAARPAPNLYLGEPPVSAETVPNQVALVAGLRTSRSP
                     FFNLLRAQPPLEPPAPPNPLPPLPQQVVDKLNALLAAPDLNLRLPLGGRRLRAETWRL
                     LRRPAAARNTPLLNRMLLADLSPDQDQFSAAPGPRGVGWARAIPLPAAAYPWRVYRTT
                     AQEDDATIQQLRVEIGQAGAGGSPRATRLAIGFHKEGKFHPCLLVDAACNVIVPGGLH
                     VNGDVIEAPIPADPADPRFLDLLKQQLQQGLAAAQGAAGGLQATITGLDQAQQGTAWP
                     YQVTVRNTGATPLIYVAAYESAAINDGVLPTRQIGQIIPDLPVGGTLTFTAKHDPLNP
                     AQGTVAVAVTVVGYGPALNAVYGTARATAPIVKAAP"
     gene            2026..3639
                     /locus_tag="VF276_17125"
     CDS             2026..3639
                     /locus_tag="VF276_17125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608627.1"
                     /translation="MSNSDIARVRYYTRQFLRTADFVDEQAYHVAMQRRHNLAGHSWG
                     IVRGLDVEQDKDARLGVSVGAAIDGYGRYVILPAPQPIEPGEFDQRGSEVLDVWLVYS
                     QQDGAPAPDGYIDCGLPANSGPGRTQEFGKVILRPPNPRLVRDPVLGTPAHRQPEAVP
                     ENDLDFAPFRVPPDDPAQSWPIFLGQIQRNKGAPVPYTIDSTGRPYAGLVGEQIKAPS
                     GLAWVQVGTAEDADAKSRFAVFLQKDRCVDPDAPPLPRLAIDEDGRIDLRGDTTVYGD
                     LTVDGGAVEFNVGYAETPQPWRVYRVQYKGDLPPDPVAPAGTGAAGKDVGFDELRIEM
                     AAPVVRHAGDPKQRNQVVIGAWSDQQSKFVPCLTIADDCSVTVQGNLIVKGQFDKIPV
                     PAPPTAEALAMSRGALFTGLAGGVNAFQTAVPPAPPAPEALLNALGGMIEALAPQVAG
                     NPAFARTVATQLAERISVAMPAVRETLRAALAAPPPGPATAAAPRPPTAGRRPRPRPA
                     AAPAPEAGPKPEPGAGAAPAGDAAAGEGK"
BASE COUNT          562 a         1323 c         1271 g          519 t
ORIGIN      
        1 ttcacgagcg gcagatccta cgcgccgccg acctgaacgt cgagcaggcg tacctgatcg
       61 gcatgcgtcg ccggcacaat tgcggcccgc attcctgggg catcgtgcgc ggcctccgcc
      121 tgcgcctcca ggccggcgat ctcgtgctgt cgcccggcgt ggccttcgac ctgtacggcc
      181 gcgaactggt cgtgcccgcg gaggtccggg ttgcggacgc ctggctggat cgtgtgggca
      241 gtcccagagt cgccgtctgg ttggcttacg cgcgggtgga tctggacata gcgccggacg
      301 gacggcccga ttgcgtcccc ggcggatacg cccgcgtgcg cgaggaggca cgcctgtgct
      361 tcacggtcgc gcccgccggc gatgaagccc agatcgatcc ccgccggccg tatcaggtgc
      421 cggatacggc cctcagcttc ggttcccagt gcgaaccgcc cgatgatccc gcggtgcgct
      481 ggccggtcta tctcggccag gcatgccggg gcgatgagaa ggccccctgg tgcctggtgc
      541 cggagggcga ccccggctac cttggggaag atggatgcgg ggacgataaa ggcacaccgg
      601 gcgctgatag acaagagata ccggaagcct gcaaggggat caccgggccg gcgcgtccct
      661 acgccggcct ccagggcgag accgtcgcct ccgcctcgcg cgtgccggtc gctgccacgg
      721 gcgcggacgg cccgccggag cgcgcgcccg ccgcgcggat gcaggtgggc gccgagcaag
      781 ataccgggac gcgctttgcc gtggagatcg ccgataagca gggcgccccg caggagcgcc
      841 tgaccatcga cagtgccggc ccgatcgcgc tgcgcggcgt ggcgcgcctt tcccgcgccg
      901 cgcggccggc gccgaatctc tatttaggcg aacctccggt tagcgccgag actgtgccga
      961 accaggtcgc cctggtcgcc ggcctgcgga cctcgcgcag ccccttcttc aacctgctgc
     1021 gagcgcagcc cccgctggaa cctccggcgc cgccgaatcc cttgccgccg ctgccccagc
     1081 aagtggtgga caagctgaac gccttgctcg ccgcgcccga tcttaacctg cgcctcccac
     1141 tcggcgggcg ccggctgcgc gcggagacct ggcgcctgct gcgccggccc gcggcggccc
     1201 gcaacacccc gctgttgaac cggatgctgc tggccgatct gtcgccggat caggaccaat
     1261 ttagtgccgc gccggggccg cgcggcgtgg gctgggcacg cgctatcccg ctgcccgccg
     1321 ccgcctatcc ctggcgggtg tatcgcacca ccgcgcaaga ggacgacgcc acgatccagc
     1381 aactgcgcgt ggaaatcggc caggcgggcg ccgggggcag cccccgcgcg acgcgactcg
     1441 ctatcggctt tcacaaagag ggcaaattcc acccatgcct cctggtggac gccgcctgca
     1501 atgtgatcgt accgggcggg ctgcacgtga acggcgatgt catcgaagcg cccatccccg
     1561 ccgatcccgc cgatccgcgc tttctggact tgctcaagca gcaactacag cagggactcg
     1621 ccgccgcgca aggcgcggcc ggcgggctac aggccactat caccggcctg gaccaggctc
     1681 agcagggcac cgcctggccc tatcaggtga cagtgcgcaa caccggcgcc acgcccctga
     1741 tctacgtcgc cgcctatgag agcgcggcga tcaacgatgg tgtcttgccc acccgccaga
     1801 tcgggcagat cataccggat ctgccggtgg gcgggacact caccttcacc gcgaaacacg
     1861 acccgctcaa tcccgcccag ggcacggtcg cggtggccgt cacggtggtc ggctacggcc
     1921 ccgcgctcaa cgcggtctat gggacggcgc gggcgactgc gccgatcgtg aaagcggctc
     1981 cgtgacggct ggtcgggacc ggcggagcaa cgaggagcat cagagatgag caacagcgac
     2041 atcgcccgcg tgcgctatta tacgcgccag tttttgcgca ccgccgattt cgtcgacgag
     2101 caagcctacc atgtggcgat gcagcggcgg cataatctgg ccggccatag ctggggtatc
     2161 gtccgcgggc tggatgtgga acaggacaag gacgcccggc tgggggtcag cgtgggtgcg
     2221 gccatcgacg gctacggccg ctatgtgatc ctgcccgcgc cccagcccat cgagccgggc
     2281 gagttcgacc agcggggcag cgaggtgctg gatgtctggc tggtctacag ccaacaggat
     2341 ggcgcccctg cgccggacgg ctacatcgac tgcggcctac cggcgaacag cgggcccggc
     2401 cgcacccagg agttcggcaa ggtcatcctg cgcccgccca acccgcgcct ggtccgtgac
     2461 ccggtcctgg gcaccccggc gcaccgccag ccggaagccg tgccggagaa tgatctcgat
     2521 ttcgctccgt tccgcgtgcc gcccgacgat cccgcccaga gctggccgat cttcctgggg
     2581 cagattcaac gcaacaaagg ggcccctgtg ccctacacca tcgactcgac gggccggccc
     2641 tacgccgggc tggtgggcga gcagatcaag gccccctccg gcctggcctg ggtgcaggtg
     2701 ggcacagcgg aagacgccga tgccaagagc cgttttgcgg tctttctgca aaaggatcgc
     2761 tgcgtcgatc ccgatgcccc gcccctgccg cgcctggcga tagacgaaga cggccggatc
     2821 gatctgcgag gcgatacgac ggtctacggc gacctcaccg tcgatggcgg cgccgtcgaa
     2881 ttcaacgtag gctatgcgga gaccccccag ccgtggcggg tctatcgcgt gcagtacaag
     2941 ggcgaccttc cccctgaccc ggtggccccg gcggggacgg gcgccgcggg caaagacgtg
     3001 ggcttcgatg aattgcgcat tgagatggcg gcgccggtag tccgccacgc gggtgatccc
     3061 aagcagcgca accaggtggt gatcggcgcc tggtcggacc agcagagtaa gttcgtgccg
     3121 tgtttgacga ttgccgatga ttgctcggtc acggtgcagg gaaatctgat cgtgaaaggc
     3181 cagttcgata agatccccgt cccggccccg cccacggccg aggcgctggc gatgagccgg
     3241 ggcgcgctgt tcacggggct ggcgggcggg gtgaatgctt tccagacagc cgtgccgccg
     3301 gcgccgcctg ctcccgaggc gctgctgaac gcccttggcg gcatgatcga agcgctcgcg
     3361 ccccaggtag cgggcaatcc cgccttcgcg cggacggtag ccacgcagtt ggcggagcgg
     3421 atcagcgtgg cgatgcccgc ggtgcgcgag accctccgcg ccgccctcgc cgcgccgccc
     3481 cccggccccg ccacggcggc agcgccccgg ccccccaccg cgggccgccg cccgagaccg
     3541 cggcccgctg ccgccccggc gccggaagca ggccccaagc cggaaccggg cgcgggggcc
     3601 gcaccggccg gcgacgcagc cgccggagag gggaaataga cgatggacgg tcgcatcggc
     3661 ctcttacatt gccgc
//
LOCUS       DASUYC010001050         2515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_640365,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001050 DASUYC010000000
VERSION     DASUYC010001050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640365"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..247
                     /locus_tag="VF276_17130"
     tRNA            174..247
                     /locus_tag="VF276_17130"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:208..210,aa:Val,seq:cac)
     gene            333..1427
                     /locus_tag="VF276_17135"
     CDS             333..1427
                     /locus_tag="VF276_17135"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX6608628.1"
                     /translation="MITYPARRALAAVACGALLALTLACDSGNPTTTPVVPATVAPTA
                     TAVPATDTVVPPTNTAVPATDTAVPPTDTVVVPTDTAVPPSDTPLPAPATVTPRPPTR
                     VPPTRTPAPAPPTATSEATAPAASATSAPGEETPAPTSEATGTPVSGPPPVVDRTKVP
                     GDMSQVIKVPPGKHQIALTFDAGAGTGYVTEILDALRKHNTHITFFITGKWAEENPDE
                     LRAIVAAGHEVANHSYSHPSFPKLTDDQMITELQKTETIIQREAGVSTKPYWRPPYGD
                     ETGHVLKVAAAQGYRSIYWTWDSLDSVGKPKTKDFILKRVTESSIELDGAIILQHIAA
                     EPSAQALPEELDRLYAKGLRVVTISELLQP"
     gene            1617..>2515
                     /locus_tag="VF276_17140"
     CDS             1617..>2515
                     /locus_tag="VF276_17140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608629.1"
                     /translation="MAMFSGLLPLLIGAGVWGWWLGPAHQQPLLWAPLPGAELRLDTL
                     SLLFLTLLLALPAPPPVPPVATGTTPRPRWIATLLTPAGLALVALAGETTGQIAGLAL
                     AALGRGLAGAPGARLAWLAAVLLLPVAWSGAFDAHPLPGRPIPVLPAELVVLALLAAT
                     AGAGAWPFAGGTPANRSVRDAWLGALYGTAALIPLLRSLGAGPWDAWGRLAVLTVGLL
                     TLASAALAALAAPDHATAWRAAWRYLVAVMLIGLGSGNPVGVAGAVVWLAAGLGGLAL
                     SGRRGWPRLVGLGALGGLPPALG"
BASE COUNT          345 a          964 c          856 g          350 t
ORIGIN      
        1 gaacccccgg tgcaggccgg tttcatcacc acggtcatcc ccggcggcct gcccatgaga
       61 atcgggcccg cgtaaaggcg gcgcggcgcg tttgcggcta gcgggcgcgc cgaatcgccc
      121 cgccttgcgc gcttgcacgc ccgcgcggga tctgctataa tagcagcgtc tgtgggcgta
      181 tagctcagtt ggtcagagcg cacggttcac atccgtgagg tccatggttc gagcccatgt
      241 acgcccaccc atgaacgcct agccccggcc cgcgccgggg ttttttcgtg gccggacaga
      301 atcgcccgtc gcgcagaaag gacacgtccc ccgtgatcac ctatcccgcc cgccgcgccc
      361 tggccgccgt ggcctgtggg gcgctgctgg ctctgaccct ggcctgtgac agcggcaatc
      421 ccaccaccac gccggtcgtg cccgccacgg tggcccccac cgcaaccgcc gtcccggcca
      481 ccgacaccgt cgtgccgccg accaataccg ctgtgcccgc cacggacact gccgtcccgc
      541 ccacggacac cgtggtggtg cccacggata ccgccgtgcc gccgtccgat acgccgctgc
      601 ccgcgcccgc gactgtcacg ccgcgcccgc cgacccgcgt cccgccgacc cgcacgccgg
      661 ccccggcccc gcccacggcg accagcgaag ccaccgcccc ggcggcctcg gcaaccagcg
      721 cgcccggcga ggaaaccccc gccccgacta gcgaggccac gggtacgccg gtgagcggcc
      781 cgccgcccgt cgtcgaccgc accaaggtgc cgggcgacat gagccaggtc atcaaggtac
      841 cgcccggcaa gcaccagatt gcgctcacct tcgacgcggg ggccggcacg ggctacgtca
      901 ccgagatcct cgacgccctg cgcaagcaca acacgcatat caccttcttc atcaccggca
      961 aatgggccga ggagaacccc gacgaactgc gcgccatcgt cgccgccggg cacgaagtgg
     1021 cgaaccacag ctactcgcac ccgtcgttcc ccaagctgac cgacgaccag atgatcaccg
     1081 aattgcagaa gaccgagacg atcatccagc gcgaagccgg cgtgagtacc aaaccttact
     1141 ggcgcccgcc ctacggcgat gagaccggcc atgtgctgaa agtggcggcg gctcagggct
     1201 accggtccat ctactggacc tgggacagtc tcgactcggt cggcaagccg aagacgaagg
     1261 actttatcct caagcgcgtg accgagtcct cgatcgagtt ggacggggcg atcatcttgc
     1321 agcacatcgc cgccgaaccg tcggcccagg cgctgccgga ggaactggac cggctctacg
     1381 ccaagggcct gcgcgtggtc accatctcgg agctgcttca gccctgagcg cgtcgccggt
     1441 gacggtgcac ccaggcgctg cgggcagcgt gtccgcagcg cctgggcata ccgagcgcca
     1501 cgccgcctgg ccgtctccgg cccacgagac gagactcttc gctgcgctca gagtgacagg
     1561 cgcgcaaggt aatgctgagc gcggcgaagc atctccgtcc agagtgacat gccccgatgg
     1621 cgatgtttag cgggctgctg ccactgctga tcggcgcggg agtctggggc tggtggctgg
     1681 gaccggcgca ccaacagccg ctgctctggg cgcccctgcc cggcgccgaa ttgcgcctgg
     1741 atacgctcag cctgctgttc ctcaccctgc tcctggcctt gcccgccccg ccgcccgtgc
     1801 cgccagtcgc gaccggcacg acgccccggc cccggtggat cgccaccctg ctcacgccag
     1861 ccgggctcgc gctggtggcc ctggcgggcg agacgaccgg gcagatcgcg gggctggccc
     1921 tggccgcgct ggggcgcggg ctggcggggg cgcccggcgc ccggttggca tggctggcgg
     1981 cggtattgct gctgccggtg gcctggagcg gcgccttcga cgcccatccc ctgccgggcc
     2041 gtccgatccc ggtgctaccg gcggaactcg tggtcctggc gctgctggcg gcgaccgccg
     2101 gagccggcgc ctggccgttc gcgggcggca ccccggccaa ccgtagcgtg cgggatgcct
     2161 ggctcggggc gctctacggc accgcggcgc tcatcccgtt gctgcgcagc ctcggcgcgg
     2221 ggccgtggga cgcctggggg cgcctggctg tgctcacggt cgggctgctc acgctggcaa
     2281 gtgccgccct ggccgccctg gccgcccccg accacgcgac agcctggcgc gcggcctggc
     2341 gctacctggt cgccgtcatg ctgatcggcc tgggcagcgg caacccggtg ggcgtggcgg
     2401 gcgccgtcgt ctggctagcc gccgggttgg gcgggttggc actgagcgga cggcgaggct
     2461 ggccgcgcct ggtcgggttg ggcgcgctgg gcggcctccc gcccgcgctc ggctt
//
LOCUS       DASUYC010001051         6082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1332626, whole genome shotgun sequence.
ACCESSION   DASUYC010001051 DASUYC010000000
VERSION     DASUYC010001051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6082
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332626"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..1142
                     /locus_tag="VF276_17145"
     CDS             231..1142
                     /locus_tag="VF276_17145"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015190912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX6608630.1"
                     /translation="MASRDIAFTREDITVALVRRLIAEQFPQWVDLPIRPVAFAGWDN
                     CTFHLGANMRVRLPSAQGYVAQVEKEHRWLPRLAPLLPLPIPVPIARGVPGADYPWPW
                     SIYRWLDGKPATIAPIADLPAFATALAQFLTALERIDATGGPAAGPHNFYRGGPLTVY
                     DAETRQAIAALDGRIDAAAATAVWETALQATWHGSPVWVHGDVASGNLLVTEGRLSAV
                     IDFGSSGVGDPACDLTIAWTFFSGESREAFRAGMPLDSATWARARGWALWKALITLVP
                     ILHTDPVKAAEPRRVIDAVLAEHERAA"
     gene            1168..2205
                     /locus_tag="VF276_17150"
     CDS             1168..2205
                     /locus_tag="VF276_17150"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX6608631.1"
                     /translation="MSMEDVARGPASRAVASPRLFAALHEHYGIPADTARQDLGGSSN
                     LNLLLGDGARRYVVRVYRPWVTEARLADLQLARRHLVQGGVPCAVPIPTRTGESWIRV
                     DDRLVEVEPYFEHDAKMDSWPRLEAGLPLLGRIHSLLRPLAVSAAGRNAPAANYIAPQ
                     DVLFGTQRGIARLRQWAPSPGELQLAAAAEELAQLVDRAQRGIEDLPRQLVHGDYWDN
                     NVLFRAGRVVFVGDLDFMGERARIDDLALTLYYTNSTFADDPVSDARIHRLRTLADAY
                     DAGAGPPLSPAERAALPVALARTPLAFIAMIAAVDSEAGARRLAAEMTADIAWALVIM
                     RDLPRWQSGFA"
     gene            2550..5636
                     /gene="treY"
                     /locus_tag="VF276_17155"
     CDS             2550..5636
                     /gene="treY"
                     /locus_tag="VF276_17155"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HEX6608632.1"
                     /translation="MVTTLQTPNPSAPAVDMPPDARVEALVDAVRARLAAGPHIPSAT
                     YRVQLNRYFTFAQAREQVEYLHRLGISDLYASPYFAANAGSLHGYDIVNHNELNPEIG
                     SQEDYDALVAELHRHSMYQVLDIVPNHMGIGQLGNTWWMDVLENGPASVYAPYFDIEW
                     DPINTKITNKVLLPILGDQYGKVLENQELRLSFVPEDGAFRLHYYDRPFPIEPGSYQA
                     VLDYDKAALIDELGAESEAALEYQSILTALQHLPPPDETDRDRVLERNREKEIIKRRL
                     ASLCAAEPRVVAHIERSVAAFNGTAGEPRSFDLLDRLLDAQPYRLSFWRIAGEEINYR
                     RFFDINELAAIQVDQLPVFEETHRTILQLLAAGKANGLRIDHIDGLLDPAGYLDDLQR
                     GYVLAVCRACMDELPESIPDAERPALEAAVLERLAAPGAPDAALRPLYTVVEKILGRG
                     ESLPADWATYGTTGYEFTNALNGLFVNRANARAFDALYTTFTGETVKFADLAYESKKQ
                     IMRLSLSSEINMLTNMLNRISQSDRRYRDFTLNSIRHAIREVIACFPVYRTYISARRP
                     VPDKRDQGFVETAVARAKKRNPATDLTIFDFLRDILVLTDYADLSEADQVAHLKFVLK
                     FQQVTGPVIAKGLEDTAFYVYNRLISLNEVGGEPGDFGLSPADFHKQQADRQRRWPAA
                     LLTTSTHDTKRSEDVRARIDVLSERPKEWRAALRRGARANRKHKRSVDGQLAPSPNEE
                     YFLYQTLLGVWPCAGDLDDAAYEDLLSRVDAYMRKALNEAKIHSSWVNTNEPYLAAVS
                     DFIRAILRRDDANRFLPDFLAFQQEIAHYGAFNSLAQVLLKITSPGVPDIYQGTELWD
                     FSLVDPDNRRPVDYGLRRRLLDEVAAVADPAGAAALVETRADGRIKLFVTSRALAYRR
                     NHVDLFQAGAYTPLAAQGRHAECSVAFARSAGDRQVITVVPVLAAGLAKSGERGLPLG
                     TVWGDTWLEVPGATAGQGFRNVFTGEVVEAQERNGKAGLALEQVLAVFPVALLERI"
     gene            5717..>6082
                     /locus_tag="VF276_17160"
     CDS             5717..>6082
                     /locus_tag="VF276_17160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme GlgX"
                     /protein_id="HEX6608633.1"
                     /translation="MLPSPDKLAVWPGDPYPLGATWDGQGVNFALFAQHATGVELCLF
                     DTPDAPHERERIAMPEQTDNVWHVYLPGVQPGQLYGYRVYGPYEPEEGHRFNPAKLLV
                     DPYARALTGRLEWNDAVFGY"
BASE COUNT         1058 a         2116 c         1938 g          970 t
ORIGIN      
        1 cacctccgtt tgtgacaaaa tcgcggcgcc atccagggcg tcagcgagaa aggcccgaaa
       61 tgagcagcaa tccgccgcgg ggggctatcc aaccgcggat gttggcatag cccctgctgg
      121 ctggtagtgc aaaccggcac atcgcactgg cgccttgcgg cagccgagtc aacgcaggca
      181 gaggcgctta caaagctggt ctagatatat tgaaaggcaa agattatttt atggcgtccc
      241 gcgacatcgc tttcacaaga gaagacatca ccgtggcgct ggtgcgccgg ttgatcgccg
      301 agcagtttcc ccagtgggtc gatctgccga tccggcccgt ggcgttcgcc gggtgggaca
      361 actgcacgtt tcacctgggt gcgaacatgc gcgtgcgcct gcccagcgcc caggggtatg
      421 tcgcgcaggt ggagaaggaa catcgctggt tgccccggct ggccccgctc ctgccgctgc
      481 ctattccggt cccgatcgcg cggggcgttc ccggcgccga ctatccctgg ccctggtcca
      541 tctaccgctg gctcgacggc aaacccgcca cgatcgcgcc tatcgccgat ctgcccgcat
      601 ttgcgacggc gctggcacaa ttcctgactg ccctggagcg catcgatgcc accggcggcc
      661 cggcggccgg cccgcataac ttctaccgcg gcgggccgct gaccgtatac gacgcggaga
      721 cccggcaggc catcgccgcg ctcgacggcc ggatcgacgc cgctgccgcg accgccgtgt
      781 gggaaacggc gctacaggcc acctggcacg gctcgcccgt ctgggtccac ggcgacgtgg
      841 cgtccggcaa cctgctggtc acggagggcc ggttgagtgc cgtcatcgac ttcgggtcgt
      901 cgggcgtggg cgatcccgcc tgtgacctga caattgcctg gaccttcttc tcaggggaaa
      961 gccgggaagc gttccgcgcc gggatgccgc tggacagtgc cacctgggcg cgggcgcggg
     1021 gctgggcgct ctggaaggcg ctgattaccc tggtcccgat cctgcacacc gacccggtga
     1081 aagccgccga gccgcggcgt gtcatcgacg cagtgctggc cgagcatgag cgcgccgcat
     1141 gagatcgcgg ctgtcaggag gtgagcgatg agcatggagg acgtggcgcg cgggccggca
     1201 tcgcgcgcgg tggcgtcgcc ccggctgttc gctgcgctgc acgagcacta cgggatcccc
     1261 gccgataccg cccggcaaga tctcggcggc tcatctaacc tcaacctgct tctcggcgac
     1321 ggcgcccggc gctatgtcgt ccgcgtctat cggccctggg tcacggaggc gcggctggcc
     1381 gatctgcaac tcgcccgccg gcacctggtg caaggcgggg tgccctgcgc ggtgcccatc
     1441 cccacgcgca ccggcgagtc gtggattcgc gtggacgacc ggctcgtcga agtcgagccc
     1501 tacttcgagc acgatgcgaa aatggattcc tggccgcggc tggaggcggg cctgccgttg
     1561 ctggggcgca tccacagcct gctgcgcccg ctcgcggtga gcgcggcagg ccgtaacgcg
     1621 cccgccgcca actatatcgc cccgcaggat gtgctcttcg ggacgcaacg cggcattgca
     1681 cgcctccggc aatgggcgcc ctcgcccggc gaactgcagc ttgcggcggc agccgaggaa
     1741 ctggcacagt tggtggatcg ggcgcagcgc gggatcgaag acctgccccg gcagttggtc
     1801 cacggcgact actgggacaa caacgtgctg ttccgcgcgg gccgcgtggt cttcgtcggc
     1861 gacctcgact tcatgggcga acgcgcccgc atcgacgacc tggcgcttac gctgtactac
     1921 accaactcca ccttcgccga cgacccggtg tcggatgcgc gcatccaccg cctacgcacg
     1981 ctcgccgacg cctacgacgc gggcgcgggc ccaccactct cgcccgccga acgggcggct
     2041 ctccccgtgg ccctggcccg gacgccgcta gccttcatcg ccatgatcgc tgcggtcgac
     2101 tccgaggccg gcgcgcgccg gctggcagcg gagatgacgg cagatattgc ctgggcgctg
     2161 gtcatcatgc gcgacctgcc gcgctggcag tcgggcttcg cctgatccga tactgttgtg
     2221 tgttggcacg acatttgctt atagcgtaca caggactgga atgtcaggat agaagctgcg
     2281 catcacggga ctagacaagc gatgatcgcg gcgatagtcg ttcctcatga gggtcaagga
     2341 cgacccgttt ctcaggtatt tgcccactta cgatcgctgt tgccctgccg ctgcgcaaca
     2401 atggtatgcg tgaatagccg ggtggtgatg agagattgat gacggataac agcgccacct
     2461 ccggcacgca ggcgcgtgag cccgatgcac agacatcgca ggcccgcctg accagggcag
     2521 gaatacggca accacaactg gaaagaacaa tggtaacaac tctacaaaca cctaacccct
     2581 ccgcgcctgc ggtggatatg ccccccgatg cgcgtgtcga agcactggtc gatgccgtgc
     2641 gcgcccggct cgccgcgggc ccgcatatcc cgtcggcgac ctatcgcgtg cagttgaatc
     2701 gctacttcac ctttgcgcag gcccgcgagc aggtcgaata cctgcaccgg ctcggcatca
     2761 gcgatctgta cgcctcgccc tactttgccg ccaacgcggg cagcctgcac ggctacgaca
     2821 tcgtcaacca caacgaactc aatcccgaga tcggcagcca ggaggactat gacgcgctgg
     2881 tggccgaact ccatcgccat agcatgtacc aggtgctcga catcgtgccc aaccacatgg
     2941 gcatcgggca actgggcaac acctggtgga tggacgtgct ggagaacggc ccggcgtcgg
     3001 tctacgcccc ctacttcgac atcgagtggg acccgatcaa caccaagatc accaacaaag
     3061 tgctgctgcc catcctgggc gaccagtacg gcaaagtcct ggagaaccag gagttgcgcc
     3121 tgagcttcgt gccggaagac ggagcgttcc gcctgcacta ctacgatcgc cccttcccca
     3181 tcgagccggg gtcctaccag gcggtcctcg attacgataa ggcggcgctc atcgacgaac
     3241 tgggcgcgga gagcgaggcg gcgctggagt accagagcat cctcaccgcc ctccagcacc
     3301 tcccgccgcc cgacgaaacc gaccgcgacc gggtgctgga gcgcaaccgc gagaaagaga
     3361 tcatcaaacg gcgcctggcg agcctttgcg ccgccgagcc gcgcgtcgtg gcccacatcg
     3421 agcgcagcgt ggcggccttc aacggcacgg cgggcgagcc gcgcagcttc gatctgctgg
     3481 accgcctgct cgacgcccag ccctaccgcc tcagcttctg gcgcatcgcg ggcgaggaga
     3541 tcaactaccg gcgcttcttc gacatcaatg aactggccgc catccaggtg gaccagttgc
     3601 cggtgttcga agagacccac cgcaccatcc tgcaattgct ggccgcgggc aaagcgaacg
     3661 gcctgcgcat cgaccatatc gacgggttgc tcgatcccgc cgggtacctg gacgacctgc
     3721 agcgcggcta cgtgctggcc gtctgccggg cctgcatgga cgaactcccc gaatcgatcc
     3781 ccgacgccga gcgccccgcc ctggaggccg ccgtgctgga gcgcctcgcc gcgcccggcg
     3841 cgcctgatgc cgctctccgg cctctctaca ccgtggtcga gaagatcctg ggccggggcg
     3901 aaagcctgcc cgccgactgg gccacctacg gaaccaccgg ctacgaattc accaacgccc
     3961 tcaacggcct gttcgtcaac cgggccaacg ccagggcatt tgacgcgctc tatacgacct
     4021 tcaccggcga gacggtcaag ttcgccgacc tggcctacga gagcaagaag cagatcatgc
     4081 ggctgtcgct gtcgagcgag atcaacatgc tgaccaacat gctcaaccgc atctcgcaaa
     4141 gcgaccgccg ctaccgcgac ttcacgctca acagcatccg ccacgccatc cgcgaggtga
     4201 tcgcttgctt cccggtctac cgcacctaca tcagcgcgcg ccggcccgtg cccgacaagc
     4261 gcgaccaggg cttcgtcgag acggccgtgg cacgggccaa gaaacgcaac ccggccacgg
     4321 acctgaccat cttcgacttc ctgcgcgaca ttctcgtgct gacggactac gcggacctga
     4381 gcgaagccga tcaggtggcg cacctgaagt tcgtgctcaa gttccagcaa gtgaccggcc
     4441 cggtgatcgc caaggggctg gaggacaccg cgttctacgt ctacaaccgc ctcatctcgc
     4501 tgaacgaggt cggcggcgag cccggcgatt tcggcctcag ccccgccgac ttccacaagc
     4561 agcaggccga ccgccagcgg cgctggcccg ctgccctgct gaccaccagc acccacgaca
     4621 cgaagcgcag cgaggacgtg cgtgcgcgca tcgacgtgct ctccgagcgc ccgaaggagt
     4681 ggcgcgcggc cctgcgccgc ggggcgcgcg ctaaccgcaa gcacaaacgc tcggttgacg
     4741 gacaactcgc acccagcccc aacgaggagt acttccttta ccagaccctg ctgggcgtct
     4801 ggccctgcgc gggcgatctg gacgatgccg cctatgagga tctcctcagc cgcgtggacg
     4861 cctacatgcg caaggcgctg aacgaggcca agattcacag tagctgggtc aacaccaacg
     4921 agccctatct ggcggcggta agcgatttca tccgcgccat cttgcgccgc gacgacgcca
     4981 atcgcttcct gcccgatttt ctggccttcc agcaggagat cgcccattac ggcgccttca
     5041 actcgctggc ccaggtgctg ctgaagatca ccagccccgg cgtgcccgat atttaccagg
     5101 gcaccgaact ctgggacttc agcctggtcg accccgataa ccgccgcccg gtggactacg
     5161 gcctgcgccg ccgcctgctg gacgaagtgg ccgcggtggc ggacccggcg ggggctgcgg
     5221 ccctcgtgga aacccgcgcc gacggccgga tcaagctgtt cgtcaccagc cgcgccctcg
     5281 cctatcgccg gaaccacgtc gatctgttcc aggcgggcgc ctacaccccg ctggccgcgc
     5341 aagggcggca cgctgaatgc agcgtggcct ttgcccgcag cgccggcgac cggcaggtga
     5401 tcaccgtggt gcccgtgctg gccgccgggc tggcgaaaag cggggaacgg gggctgccgc
     5461 tgggcaccgt ctggggcgat acctggctgg aagtgcccgg cgccacggcc gggcaggggt
     5521 tccggaatgt ctttaccggc gaggtcgtgg aagcgcagga gcgcaacggc aaggccggac
     5581 tcgcgttgga acaggtgctt gcggtcttcc ccgtggccct cctggaacgc atctaaaacg
     5641 caactgcata ggcgggcaac acaatccggc atgccgctga actcgtgccc gccgtctttc
     5701 ggaggtttgg aataccttgt taccgagtcc cgataagctg gccgtctggc cgggcgaccc
     5761 ctatcccctg ggcgcgacgt gggatgggca gggcgtgaac ttcgcgctgt tcgcgcagca
     5821 cgcgacgggt gtcgagctat gcctgttcga tacgcccgac gcgccgcatg agcgcgagcg
     5881 gatcgccatg cccgagcaga ccgataatgt ctggcatgtg tatctaccgg gggtgcagcc
     5941 ggggcagctg tatggctacc gtgtctatgg gccgtacgaa cctgaggaag gccaccgctt
     6001 caacccggcc aagctgctcg tcgatcccta tgctcgcgcg ctgaccggca ggctcgaatg
     6061 gaacgatgcc gtgtttggct ac
//
LOCUS       DASUYC010001052         6320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_873554,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001052 DASUYC010000000
VERSION     DASUYC010001052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6320
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873554"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(222..1100)
                     /gene="atpB"
                     /locus_tag="VF276_17165"
     CDS             complement(222..1100)
                     /gene="atpB"
                     /locus_tag="VF276_17165"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX6608634.1"
                     /translation="MTPPKPSFAAETLFTIGGFFHVSNSMIMTLIVAAILVIVFARAT
                     GKMQLVPGRLQNFAEVIVEGLMGIVESTAGKRTGRIIFPLIATLFIFIITANWLSLLP
                     IGWIGLCHPEAAAAGEAAHAAIHQGTCPEGAILVPFLRAPNADLNMTLSMALIAFIVV
                     QTAGVIAHGPGGYLKELTTPIFLAPVHIIGELSRIISLSFRLFGNIFGGEVLLSVMYF
                     LLGSIFLGFATIIFVGLEVLFGTIQALVFSLLTLVYISMAVAGHGDAEGHDVEHNPAG
                     EDIAEHPQTGTEVAMG"
     gene            complement(1097..1432)
                     /locus_tag="VF276_17170"
     CDS             complement(1097..1432)
                     /locus_tag="VF276_17170"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEX6608635.1"
                     /translation="MRDRNSLRALAIAGEVGFGVAGPLVICTGLGWYLDGQWGTKPWL
                     VLIGLALGLLSMFATFYRLATAFPAQPRSTKRSVPPPATQDQTGDNPDDAGDGSPPPA
                     PSGSTRSNP"
     gene            complement(1521..1922)
                     /locus_tag="VF276_17175"
     CDS             complement(1521..1922)
                     /locus_tag="VF276_17175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608636.1"
                     /translation="MDDQGAAGGCERRGGVTRRLGSEGQVRVVAVWVCADGRELRVVR
                     WELRGAARDLYQWSRGRDFQAADDIENEAAEWTAGVALEQRESATARWSTVQRQELEG
                     QDLHSVAARLRGWEAGLLDRAEKLARRVKRL"
     gene            2170..2865
                     /locus_tag="VF276_17180"
     CDS             2170..2865
                     /locus_tag="VF276_17180"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX6608637.1"
                     /translation="MIILLLGAPGVGKGTQSALLCEQLDMAHVASGDLLREHRRNGTP
                     LGKQADEYLRRGDLVPDKLVIDMILERLSAPDCARGVILDGFPRTVTQAAALDATLAR
                     QGTQVERALYIKADPQILLDRLSGRWICRGCQNSFHERFNPPRVRGVCSHCGGELYQR
                     DDDRREVAENRLQVYFEQTLPVISYYRGRGALVEVNGDQEIEAVTDDLLAALTLAPAY
                     LLMNESVRLSNFA"
     gene            3260..3565
                     /gene="rpsF"
                     /locus_tag="VF276_17185"
     CDS             3260..3565
                     /gene="rpsF"
                     /locus_tag="VF276_17185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX6608638.1"
                     /translation="MPDLREYELLYILKPTIADDQIPAAIDKVAGMVTTRGGDIYEVL
                     QTPPWGKRKLAYPIDRHVDGYYVVNHMRMDPDTTADFEHMLEINEDVIRHMLVRMDV"
     gene            3647..4093
                     /locus_tag="VF276_17190"
     CDS             3647..4093
                     /locus_tag="VF276_17190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014996077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX6608639.1"
                     /translation="MAGRGLNKVMIIGNLGGDPEMKYTPSGKAITTFRVAVGRTYRSP
                     EGENRDETEWFRVVAWDKLAETCNQYLHKGSKVYVEGRLQTRTWKDNNGQDRYMTEVV
                     ANEMMMLDSRGAGQGPSEAPARGGQSSGGGQSFGGGGDDLDDDIPF"
     gene            4189..4647
                     /gene="rpsR"
                     /locus_tag="VF276_17195"
     CDS             4189..4647
                     /gene="rpsR"
                     /locus_tag="VF276_17195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX6608640.1"
                     /translation="MDTTRPEAPAAPAPAGAADGQRPEQQPGGGGGYGGPRPGGYGGP
                     RPGGGGFGGPRPGGGGRPGGPGGRPAGRRPKSKDRFVPRRKVCGFCAEKLPYIDYKDV
                     GRLRRYLSERGKIEPRRKTGTCARHQRALTVALKRARHVALLPFVGPHAR"
     gene            4663..6195
                     /locus_tag="VF276_17200"
     CDS             4663..6195
                     /locus_tag="VF276_17200"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX6608641.1"
                     /translation="MANKRRKDAVQAPRQMTRGQLSRHQRELQRTRRFYLAMAGVGVL
                     IVLVLLTALVSQYVIRPNIEVGKVNDQTITRAMYNKYRAWTIFNQLRVLEFYAQQGDA
                     TQAQQYASQITTYQNELQHLDSWPTIDATTLQQLADNLLLEQKAGADLGVSVTDADVL
                     AAAQKNFEPQPTPIPGPTETPGTPTATATTTPTGAPPTATATATGTPPTETPTVTPTA
                     THTATPGPSPTTTSTPTITSTPLPVPGAEKTAVVDYNSFIQAIAAGPAPATGYCSLGC
                     PSLSEQDYLSLVVKPDELQKKVTEKLAANIPTTQEEVHVQHILVNADQEALAKDIKAQ
                     LDKGADFAALAAKYSTDTSNKDQGGDLGWFAPTEKGGPMVKEFSDAAFALTKPGQISD
                     PVKSQFGWHIIRLIERGPRPLTSDELDKAKSDAYSAWLSDEKAKSTIRLDIPATPTPF
                     PTQAPLPTEPPAAATATVPLTGTVGTAETPATTPVTTPATDTTGAKATAVPGSPGATP
                     TQ"
BASE COUNT         1177 a         2087 c         2038 g         1018 t
ORIGIN      
        1 ccagcccgat tgccaggccg gcggccaaac gcgctgctgt acctacgtcc atgagagaat
       61 tcctcctaaa ggttggcgtg cctgttgcgg caactcgcta attttcgtgg ggcgggaccg
      121 ccggtgagct cgtcccgccg cccgtaagcg tgcagtgcct cgcagtcgag gccgggcgcg
      181 cccgcacggc gcgcccaagg cttaccaaag tgacggcatc ctcagcccat ggcgacttcc
      241 gtgccggtct gcggatgctc ggcgatatct tcgcccgccg ggttatgctc gacgtcatgg
      301 ccctcggcgt ccccatgccc ggccacggcc atggagatat agaccagggt caagagcgag
      361 aacacaagcg cctggatcgt cccaaacagc acttccaggc ccacaaagat aatcgtcgca
      421 aagcccagga agatcgagcc gagcaggaag tacatgaccg acaacaacac ctcgccgccg
      481 aagatattgc cgaagagacg gaaggagagc gagatgatgc ggctgagttc gccgatgatg
      541 tgaaccggcg ccaggaagat cggcgtggtc agttccttaa gatagccgcc cggcccatgg
      601 gcgataaccc ccgccgtctg cacgacgata aacgcgatca gcgccatcga cagggtcatg
      661 ttcaggtcgg cgttgggcgc gcgcaggaag ggcacgagaa tcgcgccttc cgggcaggtg
      721 ccctggtgga ttgcagcatg ggccgcttcg cccgcggcgg cggcctccgg gtggcagagc
      781 ccgatccaac caataggcag cagcgacagc cagttggcgg tgatgatgaa gataaacagg
      841 gtggcgatga gcggaaagat gatgcgcccg gtgcgcttgc cggcggtgct ttcgacaatg
      901 cccatcaggc cttcgacaat cacttcggcg aagttttgca ggcgccccgg caccagttgc
      961 attttgccgg tcgcgcgggc gaagacgatg accaggatgg ccgcgacgat cagcgtcatg
     1021 atcatcgagt tgctgacgtg aaaaaaccca ccgatagtga acaatgtttc ggctgcgaag
     1081 ctgggcttcg gaggcgtcat gggttgctcc ttgtcgatcc ggaaggggcc ggcgggggcg
     1141 aaccgtcgcc ggcgtcgtcc gggttatcgc ctgtctggtc ctgggtcgcg ggagggggca
     1201 cactgcgttt cgtggatcgg ggttgcgccg ggaaagcggt tgccagccgg taaaatgtgg
     1261 cgaacatgct cagcaatccc agtgccagcc cgatcaacac cagccacggc tttgtgcccc
     1321 attgcccgtc gagataccac ccgaggcccg tgcagatcac taacggcccg gccacaccaa
     1381 agccgacttc gccggcgatg gctaacgcgc gcagggagtt gcgatcacgc atcagatcct
     1441 cgcaatcaac gtatccaatt ggtcggcgca ttctgtaagc gcccgcatta tagcactgtc
     1501 aggcatgatt gtcaagattt tcacaatctt ttcacccgcc gggccagctt ctccgcgcga
     1561 tccagcagcc cggcttccca gccgcgcaac cgcgcggcca cactgtgcaa atcctgaccc
     1621 tccagctcct ggcgctgcac ggtgctccac cgcgcggtgg ccgattcccg ctgctccagc
     1681 gcgaccccgg cggtccactc cgccgcctcg ttctcgatgt catcggcggc ctggaagtcg
     1741 cgcccgcggc tccattggta gagatcgcga gccgcgccgc gcagttccca gcgcaccacg
     1801 cgcaactcgc ggccatcggc gcagacccag acggcgacca cccgcacctg gccttcgctc
     1861 ccgagccggc gcgtgacgcc gccccggcgc tcacacccgc cggctgcccc ctggtcgtcc
     1921 attgaacgct agtctgtagc aggattcagg ccatgcattt cgatgttgcc cgccccattt
     1981 ggaggcctgg ggcacttgcg ttgcgccgtg gtttccgtta tgctatataa tgttcggcgg
     2041 taccagccat ccgggtcgct cagagcgtag tgcggagtcg cgtcatgtgt cactctgagc
     2101 ggagcgaaga gtctcgtccg ccggtacata ccaccaacag tcctggcaat ggagacaggg
     2161 aggtcaatga tgatcatact actgcttggt gccccaggcg tgggcaaagg aacgcaatcg
     2221 gctctgctct gcgaacagct tgatatggct catgtcgcca gcggcgatct gctgcgcgag
     2281 caccgccgca acggcacgcc tctcggcaag caggccgacg agtatctgcg gcgcggcgac
     2341 ctggtgcccg ataagctggt catcgacatg attctggagc gcctcagcgc gcccgattgc
     2401 gcccgcggcg tcatcctgga cggcttcccc cgcaccgtca cccaggccgc cgcgctcgat
     2461 gccacgctgg cccggcaggg cacgcaggtg gaacgcgcgc tctacatcaa ggccgacccg
     2521 cagatcctgc tcgaccggct gtcgggccgc tggatctgcc gcgggtgcca gaacagcttc
     2581 cacgagcgct tcaatccgcc ccgcgtgcgt ggggtgtgca gccactgcgg cggcgaactc
     2641 taccagcgcg acgacgaccg ccgcgaggtg gccgagaacc gcctgcaggt ctatttcgag
     2701 cagaccctgc ccgtgatctc ctattatcgc ggccggggcg ccctggtcga ggtcaatggc
     2761 gaccaggaga tcgaggccgt cacggacgac ctgctggccg cgctcacgct cgcgcccgcg
     2821 tacctgctta tgaacgaaag cgtccggctg tccaatttcg cctgatcgct tcccccgcta
     2881 tccgagaggg cgcatgcttt gccatgcgcc ctctttcatg cgcggcagca ttggacacat
     2941 tcgtccggaa cggcaccgtg aagatcggtt atattaactt cgttcttcgc agccttcgcg
     3001 tcttcgcggt gaaacggtga acgaatgacc ggatgccgcg tttgcgctgc ccgcccggtt
     3061 atggtataat cacggttggc gtgcgtcacg cacgcgaaaa tcatatttgt ccgggccttg
     3121 cgcttctttt gtgaagcgac tctctagatc gcgtctcagc gatcccggcc cgcgaccttt
     3181 ctgctcagcg tagtccgggt ggcggcacac cccatccgtg tttagcgctg ggtaaaccca
     3241 aggaaaggag gtgtgtacga tgcccgatct gcgcgaatac gaactgctgt atatcctcaa
     3301 gccgaccatc gccgacgatc agatccctgc tgccatcgac aaggtggccg gcatggtgac
     3361 gacgcgtggt ggcgatatct acgaggtgct gcagaccccg ccctggggca agcgcaagct
     3421 ggcctatccc atcgatcgtc atgtcgacgg ctattatgtc gtcaaccata tgcgcatgga
     3481 ccccgatacg actgctgact ttgagcacat gctcgaaatc aacgaagacg tgatccgcca
     3541 tatgctggtg cggatggacg tttaagtcct ggcgcgtccc gactagtgtg gtgtagtgtg
     3601 taatgtcgcg tttgtgtgag tagcttgatc actaggaggc tttaacatgg cgggtcgtgg
     3661 actgaacaag gtcatgatca tcggcaatct gggtggcgat ccggaaatga agtatacgcc
     3721 gagcgggaaa gcgatcacga cgtttcgtgt ggctgtcggc cggacctacc gcagccccga
     3781 aggggagaat cgcgacgaaa ccgaatggtt ccgggtcgtg gcctgggata agctggctga
     3841 aacctgcaac cagtatctgc ataaaggcag caaggtctat gtcgagggtc ggttgcagac
     3901 ccgcacctgg aaggacaaca acggccagga tcgctacatg accgaagtgg ttgccaatga
     3961 aatgatgatg ctcgacagcc gcggggctgg ccaggggccg agcgaggcgc ccgcacgggg
     4021 cggccagagt tccggcggcg gccagagctt cggcggcggg ggcgacgacc tggacgacga
     4081 cattcccttc tagggagtgc agcgtgcggg cgggtcccgg tcgctcatac atggaatgaa
     4141 tgggaaggac cggcgcgcta atcgccggtc ttgaaggagg atacattagt ggatacaaca
     4201 cgtccggagg cgcccgccgc gccggcaccc gcgggtgccg ccgacgggca gcgtcctgag
     4261 cagcagccgg ggggcggtgg gggctatggt ggcccgcggc ccggtggtta tggcggcccg
     4321 cggcccggtg gcgggggctt tggcggcccg cggcccggtg gcggcgggcg accggggggt
     4381 cccggtggcc ggcccgcggg ccggcggccc aagtcgaagg accgcttcgt accgcgccgc
     4441 aaggtctgcg gcttttgcgc cgagaagctg ccctacatcg actacaagga tgtcggacgg
     4501 ctgcgccgct acctgtcgga gcgcggcaag atcgaacccc ggcgcaagac gggcacctgc
     4561 gcccgtcatc agcgcgcgct gaccgtcgcc ctgaagcgcg ctcgccatgt cgccctgctg
     4621 ccctttgtcg ggccgcacgc gcgctaggaa gcgctgccca ctatggctaa caagcgacgt
     4681 aaagatgcag tgcaggcgcc gcgccagatg acgcggggcc agctctcgcg ccaccagcgc
     4741 gagttgcagc gaacgcgccg gttctacctg gcaatggccg gcgtgggcgt gcttattgtg
     4801 ctcgtgctgc tgaccgccct ggtctcgcaa tatgtgatcc ggcccaatat cgaagtgggc
     4861 aaggtcaacg accagacgat cacccgcgcc atgtacaata agtaccgcgc ctggacgatc
     4921 ttcaaccagc tccgcgtgct ggagttctac gcgcagcagg gcgatgcgac ccaggcccag
     4981 cagtatgcca gccagatcac cacgtatcag aacgagttgc agcacctgga tagttggccg
     5041 accatcgacg cgaccacgct gcagcaactg gccgacaacc tgctgctgga gcagaaggcc
     5101 ggcgccgacc tgggcgtgag cgtgaccgat gccgacgtgc tggccgcggc gcagaagaac
     5161 ttcgagccgc agccgacccc gattccgggg ccgaccgaaa cgcccggcac gccgacggcc
     5221 accgcgacga cgaccccgac cggggcgccg cccaccgcga cggcgaccgc gaccggcacc
     5281 ccgccgaccg aaacgccgac ggtgacgccg acggccacgc acacggccac gcccggtccc
     5341 agcccgacga cgacctcgac gccgacgatc accagcaccc cgctgcccgt gcccggcgcg
     5401 gagaagacgg cggtggtcga ctacaacagc ttcatccagg ccatcgcggc ggggcccgcg
     5461 ccggccacgg gctactgctc gctgggctgc cccagtctgt ccgagcaaga ctacctcagc
     5521 ctggtcgtca agcccgatga actgcagaag aaggtcaccg aaaagctggc ggccaacatc
     5581 ccgacgaccc aggaagaagt gcacgtgcag cacatcctgg tgaatgcgga ccaggaggcc
     5641 ctggcgaagg acatcaaggc ccagctcgac aagggtgcgg actttgcggc cctggccgcg
     5701 aagtattcga ccgacaccag caacaaggac cagggcggcg atctgggctg gttcgccccg
     5761 accgagaagg gcggcccgat ggtgaaggag ttctccgacg ccgcgttcgc cctgaccaag
     5821 cccggccaga tcagcgaccc ggtgaagagc cagttcggct ggcacatcat ccgcctgatc
     5881 gagcgcggcc cccggcccct gaccagcgac gagctggaca aggccaagag cgacgcctac
     5941 agcgcatggc tgagcgacga gaaggccaag tcgaccatcc gcctggacat cccggcgacg
     6001 ccgacgccct tcccgacgca ggcgccgctg cccacggaac cgcccgcggc ggccacggcc
     6061 accgtgccgc tgaccggcac ggtggggacc gcggagactc ccgccaccac gccggtgacc
     6121 acgccggcga cggacaccac gggcgccaag gcgacggccg tgccgggtag ccccggcgcc
     6181 acgccgacgc agtagcggcg cgccgcgcgc aaaaaaagag agggtgcagc tccggctgtg
     6241 ccctctcttt ttatatgcac ttgtctggtg ggatccgctc aggcttccgg cttgcggcgg
     6301 acgtggagca cgatcccggt
//
LOCUS       DASUYC010001053         4970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_254525,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001053 DASUYC010000000
VERSION     DASUYC010001053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4970
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254525"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VF276_17205"
     CDS             complement(<1..536)
                     /locus_tag="VF276_17205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX6608642.1"
                     /translation="MEGGGKRRIDQQHAKGKLTARERIDLLLDEGTFTELDAFVVHSN
                     SDFGLADQRILGDSVVTGFGRIEGRLVFVFSQDFTVFGGSLSLVHAEKVCKIMDMAMK
                     AGAPVIGLNDSGGARIQEGVDSLGGYADIFLRNTLASGVIPQISAILGPCAGGAVYSP
                     AITDFILMVKDTSYMFVTG"
     gene            complement(804..3188)
                     /locus_tag="VF276_17210"
     CDS             complement(804..3188)
                     /locus_tag="VF276_17210"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEX6608643.1"
                     /translation="MVSPLRRSIAWLLRLLLVCASLVIALQADLIPKLLRSLAGWATP
                     QWPPQATDATPLTPGLRLEDGLHALYTTLTLGQPLLPWLIWVGAAVGLLALARPAQPA
                     QQPPSVPPVVQPRRRLVTNLLTGAGLLAILGVGFATRAANLLPDSTGTWPASNFDEQV
                     YYTNARLLAQGELPYRDHFLAHPPGVAYALAPGLAAEPAWGGPAAFAAARQWTLLFSL
                     SAIPLIFLAGRRLGGNGSGLLAAAVLALDGRVAGVAVLETLVNFWSLAAIVCYLYMLR
                     ALLRRQYVGWVLAYGICCAAALLTKIPGLAIAGALPLHALLRRRFAVAAGLVAATMGA
                     ALLGVLPFLLLAPGALVRQTIFFQLLRPQEVRPGIDQTLRIAAYPESLLTTLLAAAGL
                     GLLAAAVWRATCARPLTTHAARLQDALAGWLVAPLWALPVLAVFVFGRSYHAPYYVQW
                     APPLALLAGAICARPVWRALLPGRPRWRVRLALAGVPLAALALLPIGGTAWTATFQVP
                     SDTVYLPAGAALRAATGPHDPVLSFDPGYAFVAGRPPAVVPGGGRLVDSAGYMTYAGL
                     GIDRRPYGDLLNRVLRFDKSRNAPEIFQSAPAQAGALGGFWTSRAVVVDGKIGLPQLR
                     GQTLALLDNLAAGKQTIEHVTIYHLDPTPPPPRPMGPLTLRALTLEPLNPDGSGAGAV
                     LMQDDPTEVAVQPGQVLQLGCYWQARPGAPDARIVIRLTTATGESLLQIDRAPDQDQA
                     ATHLWNPAFVYPDLHNLPIPPTLPPGTYGLQLGINAPAAAFPPAMLPLRLIVGP"
     gene            complement(3199..3281)
                     /locus_tag="VF276_17215"
     tRNA            complement(3199..3281)
                     /locus_tag="VF276_17215"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3246..3248),aa:Leu,seq:taa)
     gene            complement(3369..3800)
                     /locus_tag="VF276_17220"
     CDS             complement(3369..3800)
                     /locus_tag="VF276_17220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608644.1"
                     /translation="MSQQETAQALVQVLSEHRDDLITMLVEQAPAAGSGYEQTPPDAL
                     RPRMASVVDACIASLARQDPGLLRGFMRAAAEQRIREGYSVDSLITLALFTEGALSDT
                     AEIAFEAAPERRKEARKLIRALIAAAEEVLDPIAASTPDES"
     gene            complement(3879..3953)
                     /locus_tag="VF276_17225"
     tRNA            complement(3879..3953)
                     /locus_tag="VF276_17225"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3919..3921),aa:Gly,seq:gcc)
     gene            complement(4011..>4970)
                     /locus_tag="VF276_17230"
     CDS             complement(4011..>4970)
                     /locus_tag="VF276_17230"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX6608645.1"
                     /translation="IEMAEPIALRVSGFVAILARVMWPAVAFLSGTTNLVVRLLGGRR
                     NAQIPSVTEEEIISMVETGEDEGVLTPSEQEMIRNIFEFTDRRVSEVMVPRPDMFELS
                     GRVTIAEAGPLVRKSGYSRIPIYEQDRENITGMVFAKDVLGAYIEGRIDQPLQKIVHP
                     VVFVPETKQIGELLQELQRSRQNLAIAVDEFGGVSGMLTLEDLLEEIVGDIQDEFQHE
                     EAPLQRVAPDELLVAGNLLLREVVDAFDLRVRDWDLDSTVAGLILELQGRIPDPGDSI
                     TWHGMTFTVAEMNGRRIQKVRVKLDEAPSVPAEEPGTTVAAGE"
BASE COUNT          829 a         1573 c         1818 g          750 t
ORIGIN      
        1 ccggtgacga acatgtagct ggtgtctttg accatcagga tgaaatcggt gatggctggg
       61 gagtagaccg cgccgcccgc gcacggcccc aggatggccg agatctgcgg gatgacgccg
      121 ctggcgagcg tgttgcgcaa gaagatgtcg gcatagccgc ccaggctgtc cacgccttcc
      181 tggatgcgcg ccccgcccga gtcgttgagg ccgatgaccg gggcgcccgc tttcatcgcc
      241 atgtccatga ttttgcagac tttctcggcg tggaccagcg agagcgagcc gccgaagacg
      301 gtgaagtcct gggagaagac gaagaccagc cgtccctcga tgcgcccgaa gccggtcacg
      361 accgagtcgc cgaggatgcg ctggtcggcg aggccgaagt cgctgttgct gtggacgaca
      421 aaggcgtcga gttcggtgaa ggtgccttcg tccagcagga ggtcgatacg ttcgcgggcg
      481 gtcagtttgc ccttggcgtg ttgttggtcg atgcggcgtt tgccgccacc ttccatcgct
      541 ttttcgcgca gttcccgcag gtgagtaatg cgggcggcgg tgccggtggc ggggtgctca
      601 atggtcttat cggacgattc aggcatgcgt gtcggtgttc ctcctggtct cctggcgggg
      661 atgggccagc ggtagtatag cacaggcgcc cgcgggggcg caagaatggg tgacgggcct
      721 aaccccctgg cccccttccc ggcgcgggaa gggggaaatt gggttataca tatcccgtga
      781 ggacaagaga aatggacgaa cagttagggc ccgactatga gccgcagcgg taacatggcc
      841 ggggggaacg cagcggcggg ggcgttgatg ccgagttgca ggccgtaggt gccgggcggc
      901 agggttggag ggatgggcag gttgtgcagg tcggggtaga cgaaggcggg gttccagagg
      961 tgggtggccg cctggtcctg gtcgggcgcc cggtcgatct gcaagagcga ctcgcccgtc
     1021 gccgtggtca gccggatgac gatgcgggca tcgggggcgc cgggccgggc ctgccagtaa
     1081 cagccgagtt gcagtacctg gccgggctgc acggcaacct cggtgggatc gtcctgcatg
     1141 agcacggcgc ccgcgccgct gccgtcgggg ttgagcggct ccagggtcag ggcgcgcagg
     1201 gtcagcggcc ccatggggcg gggcggtggc ggggtggggt ccagatggta gatggtcacg
     1261 tgctcgatgg tttgcttgcc ggcagcgagg ttatcgagca aggccagggt ctgcccgcgc
     1321 agttgcggca ggccgatctt gccgtccacg accacggccc ggctggtcca gaagccgccg
     1381 agcgctccgg cctgcgccgg ggctgactgg aagatctccg gcgcgttgcg cgacttgtcg
     1441 aagcgcagga cgcggttgag caggtcgcca taggggcgcc ggtcgatgcc gaggccggca
     1501 taggtcatgt acccggcgct atcgaccaga cgcccgccgc ctgggaccac cgctggtggc
     1561 cggcccgcga cgaaggcata gccggggtcg aagctgagca ccggatcgtg cgggccagtg
     1621 gcggcgcgga gggcggcgcc ggcgggcagg tagacggtgt cggacggcac ctggaaggtg
     1681 gcggtccagg ccgtgccgcc gatgggcaac agggccaggg cggccagcgg cacaccggcc
     1741 agggctaagc gcacgcgcca ccggggccgg cccggcagca acgcgcgcca caccgggcgc
     1801 gcgcaaatcg ccccggccag cagggccagg ggcggcgccc attgcacata gtagggcgcg
     1861 tggtaggacc gcccgaagac gaacacggcc agcaccggca gcgcccacag cggcgcgacc
     1921 agccatccgg ccagggcatc ctggaggcgc gccgcatgag tggtcagggg gcgggcgcag
     1981 gtggcgcgcc agacggcggc ggccagcagg cccagccccg cggcggccag cagcgtggtg
     2041 agcaggcttt cgggataggc ggcgatgcgc agcgtttggt cgatgccggg ccgcacctct
     2101 tggggccgca atagctggaa gaagatggtc tggcgcacga gggcgccggg cgccagcagt
     2161 aggaacggca acacgccgag cagggccgcc cccatcgtcg cggcgaccaa cccggcggcg
     2221 accgcgaagc ggcggcgcag cagggcgtgc agcggcaacg ccccggcgat ggcgaggccg
     2281 gggattttcg tgagcagcgc cgctgcgcaa catatcccat aagccaaaac ccaaccgaca
     2341 tactggcgac gcaatagagc acggagcata taaagatagc aaactatcgc cgccagggac
     2401 cagaaattga ccagggtttc gagcaccgcg accccggcca cgcggccatc gagcgccagc
     2461 accgcggcgg ccaggagtcc gctgccgttg cccccgagcc ggcgcccggc caggaagatc
     2521 agcgggatag cggacaggct gaacagcagt gtccactggc gggcggcggc gaaggcggcg
     2581 gggccgcccc aggccggttc ggcggccagt ccgggcgcca gtgcataggc gacgccgggc
     2641 gggtgggcaa ggaagtggtc gcggtagggc agttcgccct gggccagcag gcgggcgttg
     2701 gtatagtaca cctgctcgtc gaagttggac gcgggccagg tgccggtgct gtcgggcagc
     2761 aggttggcgg cgcgcgtggc gaatccaacg ccgaggatcg ccagcaaccc agcgccggtc
     2821 agcaggttcg tgacgagccg gcggcggggc tggacaacag gtggcacgga cggcggttgt
     2881 tgggcgggct gcgccgggcg ggccagggcg agcagcccca cggcggcgcc gacccagatc
     2941 agccagggca gcagcggctg gccgagggtg agggtggtat acagggcgtg caggccgtct
     3001 tccaggcgca ggcccggcgt cagcggagtg gcgtcggtgg cctgtggcgg ccactggggg
     3061 gtggcccacc cggccagcga gcgcagcagc ttggggatca ggtcggcctg gagcgcgatg
     3121 accagggatg cgcagacaag taaaagccgg agcagccatg cgatgctccg gcgtaggggt
     3181 gagaccataa aatcagggtg ccagagaggg gagtcgaacc cctacggcct tgcggccagc
     3241 ggtgtttaag accgcagcgt ctgccattcc gccactccgg ctcgtccttc cgtgagtaat
     3301 tgtcgcctac gcgctgggct ggccggtttg aaaccggccc tacatcgcac tatcaggcgg
     3361 gccggctact agctctcgtc gggggtgctc gcagcgatcg ggtcgagcac ttcctcggca
     3421 gcagcgatca gcgcacggat gagcttgcgc gcttccttgc gccgctccgg cgcggcctcg
     3481 aaggcgatct ccgcggtgtc gctgagcgcg ccctcggtga agagcgccag cgtgatcagc
     3541 gaatcgaccg agtagccctc gcggatgcgt tgctcggcgg ccgcgcgcat aaaaccgcgc
     3601 agcaagccgg ggtcctggcg cgcgaggctg gcgatacagg catcgaccac gctcgccatg
     3661 cgaggccgca atgcgtcggg cggggtctgc tcatagcccg accccgcggc gggcgcctgt
     3721 tccaccagca tcgtaatcaa gtcatcgcgg tgctcgctca gaacctgaac cagtgcctgt
     3781 gccgtttctt gctgcgacat gcctctaccc cgtgtgtgct atgcttgcgg cccaaacaaa
     3841 aaggggaagc gctcggcttt cccctctttg tgctcgaatg gagcggaaga ccagattcga
     3901 actggcgacc ctctccttgg caaggagatg ctctaccact gagccacttc cgcgtttgcg
     3961 accgtattat accaaacggc acgcctgctg tcaagagaac gccggcgggt ttactcgccc
     4021 gcggccaccg tggtgcccgg ttcctcggcg ggcacggacg gcgcttcatc cagcttgacg
     4081 cgcactttct ggatgcgccg cccgttcatc tcggccacgg tgaaggtcat accgtgccag
     4141 gtgatagagt cgccgggatc ggggatgcgg ccctgcaact ccaggatcaa cccggccacc
     4201 gtgctgtcga gatcccaatc gcgcacgcgc aggtcgaacg cgtcgacgac ctcgcgcagc
     4261 aggaggttgc cggccaccag taactcgtcg ggcgcgacgc gctgcaaggg cgcttcctcg
     4321 tgctggaact cgtcctggat atcgccgacg atctcttcga gcaggtcttc cagcgtcagc
     4381 atgcccgaca cgccgccgaa ctcgtccacg gcaatggcca ggttctggcg gctgcgctgg
     4441 agctcctgca acaactcgcc gatctgcttg gtttcgggca cgaagacaac cggatggacg
     4501 attttctgca acggctggtc gatgcgccct tcgatatagg cgcccagcac gtccttggcg
     4561 aagaccatgc cggtgatgtt ctcgcggtcc tgctcgtaga tcgggatgcg cgagtagccc
     4621 gacttgcgca ccagcggccc cgcttcggcg atggtcacgc ggcccgatag ctcgaacatg
     4681 tcggggcgcg gcaccatgac ctcactgacc cgccggtcgg tgaactcaaa gatgttgcgg
     4741 atcatctcct gctcgctcgg cgtcagcacc ccctcgtctt cgccggtctc gaccatcgag
     4801 atgatctctt cctcggtcac gctcggtatc tgcgcgttgc ggcgcccgcc cagcagccgg
     4861 acgaccaggt tcgtggtgcc ggagaggaag gcgacggcgg gccacatgac gcgcgccagg
     4921 atcgccacaa acccggagac gcgcaaggca atcggctcgg ccatctcgat
//
LOCUS       DASUYC010001054         2278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1566220, whole genome shotgun sequence.
ACCESSION   DASUYC010001054 DASUYC010000000
VERSION     DASUYC010001054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1566220"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1499
                     /locus_tag="VF276_17235"
     CDS             <1..1499
                     /locus_tag="VF276_17235"
                     /inference="COORDINATES: protein motif:HMM:NF018471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HEX6608646.1"
                     /translation="VGRLLQGRAAPPTYFLTRWLFLRLLGLVYLFAFGALWPQLPGLI
                     GPNGILPAAGYLHVFALQSGPERFLQLPTLAWLGTSDGFLLFLCGAGMLLALLVIAGV
                     LPGPALALLWLDYLSIANIGQDFLAFQWDSLLLETGFLAIFFAPWGLRPGFAHEAPPS
                     RVVLWLLRWLLFRLMFFSGVVKLASGDPTWHNLTALDYHWWTQPLPTPVAWYLYQLPD
                     WFQALSTASTFVAELFAPLLIFAPRRVRFLGAALMIALQLLIMLTGNFGYFNLLTIAL
                     CVPLLDDALLSRVFPADLRARLLAHFGRRTAPGARLLVVVPQAILILLLSWVQVSRQI
                     SVGVDVPLPVLELSGRLAPWQLVNGYGLFAIMTTDREEVIVEGSDDGTHWREYTFFAK
                     PGDVRRAPAWVAPYHPRLDWQMWFAPFGGPGANPWLTSFLRGLLRGTPQVLALLPANP
                     FPAAPPRYVRASLYAYRFTDIPTRLATGAWWQRRFVAPYIAPMSSAGP"
     gene            2004..>2278
                     /locus_tag="VF276_17240"
     CDS             2004..>2278
                     /locus_tag="VF276_17240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608647.1"
                     /translation="MDRFNRDSAPPWSAFGAMRRIQVRALGYDAQPAQRWTSWVLDDS
                     PDCLVTYRPPQGPIFRRREITWPNPAPAITLFWPARCYHLSYLLDGES"
BASE COUNT          316 a          805 c          700 g          457 t
ORIGIN      
        1 gggtggggcg gctgctccag gggcgcgccg ccccaccgac ctactttctc acccgctggc
       61 tcttcctgcg cctgctagga ctggtgtacc tgttcgcgtt cggtgcgctc tggccgcaac
      121 tgcccggcct gatcggcccg aacggcatcc tgcccgccgc cggctatctg cacgtcttcg
      181 ccttgcagag cggaccggaa cgcttcctgc aactcccgac cctggcctgg ctgggcacga
      241 gcgacggctt cctgctcttc ctttgcgggg cgggcatgct gctggccctg ctggtcatcg
      301 cgggggtgct gccggggccg gccctggcgc tgctgtggct cgattacctt tccatcgcca
      361 atatcgggca ggatttcctg gccttccagt gggactcctt gctgctggag acgggcttcc
      421 tggcgatctt cttcgcaccc tggggactgc ggcccggctt cgcccatgag gcgccgccct
      481 cgcgcgtggt gctctggctc ttgcgctggc tgctgttccg cctgatgttc ttttcgggcg
      541 tggtcaaact cgccagcggc gaccccacct ggcacaatct caccgccctc gattatcact
      601 ggtggaccca gccgctgccc acgccggtcg cctggtatct gtaccagttg ccggactggt
      661 tccaggcgct gtccaccgcg tccaccttcg tcgccgagct attcgcgccg ctgctgatct
      721 tcgcgccccg ccgcgtgcgc ttcctgggtg ccgccctgat gatcgcgctg caactgctga
      781 tcatgctcac cggcaatttc ggctatttca acctgctcac catcgccctg tgcgtcccgc
      841 tgctggacga tgcgctgttg agccgggtct tccccgcaga cttgcgcgcc cggctgctcg
      901 cgcacttcgg gcgccggacc gctccgggcg cccggctcct ggtcgtcgtg ccgcaggcga
      961 tcctgattct gttgctcagt tgggtccagg tcagccggca aatatcggtg ggcgtcgatg
     1021 tgccgctgcc cgtgctcgaa ctcagcgggc ggctcgcgcc ctggcaactg gtcaacggct
     1081 acgggctgtt tgccatcatg accacggatc gcgaggaagt gatcgtcgag ggcagcgacg
     1141 acggcaccca ctggcgggaa tacacgtttt tcgccaagcc cggcgatgtg cggcgcgcgc
     1201 ctgcctgggt cgccccgtat cacccgcggc tcgactggca gatgtggttc gcgcccttcg
     1261 gcggccccgg cgccaatccc tggctgacca gcttcctgcg cgggctgttg cgcgggacgc
     1321 cgcaggtgct ggccttgctg cccgccaacc ccttccccgc tgccccgccg cgttatgtcc
     1381 gcgcctccct ctatgcctac cggttcaccg acatccccac tcgcttggct accggcgcct
     1441 ggtggcagcg ccgcttcgtc gcgccctaca tcgcgcccat gtccagcgcc ggcccctgag
     1501 tagagcgtcg gacccaactg tcattctgag cggagcgaag aatctcgtct cggagcgggg
     1561 tagtgcggcg ccgcgactct gcgctgcact cagagtgaca agcccatcgg tgtcattctg
     1621 agcggagcga agaatctcgt cttggagcgg agtgctgcgg cgacgagacg cttcgctgcg
     1681 ctcagagtga catggataag gggaaaggct attgatcgat caaaatccgc ttgcgtgtac
     1741 ccacaaccga actctgcgcc agcactcggt gtaatactca atagcgggaa cttttggcgg
     1801 gccggcaacg tctgcacaag agaaccggcc ggtacgcgat ccctgtgcta tccggggatt
     1861 gtgcagcttg catataggat tccggtgcta ctttcgatat agtagacata ggaccattgg
     1921 gctatgcggt gcgcatagca gcaagcaccc tggcacgacg ggatccggcc agatcgcaca
     1981 ccatttcggg aaggatccgc agtatggaca ggttcaatcg cgactcggcc ccgccctggt
     2041 ccgctttcgg cgccatgcgg cgcatccagg tgcgggcgct cggctacgac gcccaaccgg
     2101 cgcaacgctg gacaagctgg gtgctggacg atagcccgga ctgcctcgtc acctatcgcc
     2161 cgccgcaggg ccccatcttc cgccgccgcg agatcacctg gcccaacccg gcgccagcga
     2221 tcacgttatt ctggccggcg cgctgctacc atctctccta cctcctcgat ggcgaatc
//
LOCUS       DASUYC010001055         4930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1105130, whole genome shotgun sequence.
ACCESSION   DASUYC010001055 DASUYC010000000
VERSION     DASUYC010001055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1105130"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..846
                     /locus_tag="VF276_17245"
     CDS             79..846
                     /locus_tag="VF276_17245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX6608648.1"
                     /translation="MTKMLPVLDPRVRAEVLAQPYSLLFVTISGAHLYGFPSPDSDYD
                     LRGAHILPLPDVVGLEPGQNTVTVSAVREGLEVDLVSHDVHKFFTLMLKRNGYVLEQL
                     YSPLVVHTTPEHAELRAIARGCITRHHSHHYRGFAATQWGLFEKERPRRVKPLLYVYR
                     VLLTGIHLMRTGEVEANLLRLNETFRLPYIADLVARKLAGPEHGTLDAVDVAFHAQEY
                     QRLWALLEEAHLASTLPEAPSARPALHDLLVRLRLGA"
     gene            906..1646
                     /locus_tag="VF276_17250"
     CDS             906..1646
                     /locus_tag="VF276_17250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608649.1"
                     /translation="MTRPVSTTENRRWGRGCLLAGLLLAACADSGPAPTPTAVAVGGN
                     AALATAIPATSTAIPIPATPTIFLLPDARTKLAAVKTVDPAAYATFYAREVAREQRML
                     TPRPSNTPQPTPLPITATPQLGLWTDCGHGVHGDPLPANCWQLFINGTLYRVMAGRLE
                     PESEADQGVLWVKRLDQRSQDVPWYLTPQRVGPVSIVAVDGLRFTVATDDPPNPPVSF
                     VFDLATRQWLHPDGTPWPTPTPLPTMPP"
     gene            1722..2240
                     /locus_tag="VF276_17255"
     CDS             1722..2240
                     /locus_tag="VF276_17255"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX6608650.1"
                     /translation="MDDLLQEVRTVLITTAARWLSMTATLSPAALARPPAPGEWSAAE
                     CLGHLIQTERDVFPRRVRNFLAGEDLSGFTPSGATGDADLSLRDLAAEFARLRAANLE
                     LIADIQPADLGRSARHPELGPVTLGEVLHTWAAHDLNHTMQAERALMQPFIAGSGAFR
                     VRFAEHVLPKQA"
     gene            2364..>4930
                     /locus_tag="VF276_17260"
     CDS             2364..>4930
                     /locus_tag="VF276_17260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0051731 - polynucleotide
                     5'-hydroxyl-kinase activity [Evidence IEA];
                     GO_process: GO:0042245 - RNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide kinase-phosphatase"
                     /protein_id="HEX6608651.1"
                     /translation="MNITIPELALVVLIGASGAGKSTFARTHFRPTEILSSDFCRGLV
                     ADDENDQSATTDAFDVLHYIAAKRLAAGRLTVVDATSVQPEARKPLVDLAREYHCLPV
                     AIVFNLPERLCHERNQGRPDRAFGPHVVRRQVQQLHRSLRGLRGEGFRHVFVLDTPEE
                     VAAVTVERQPLWNNRRTEHGPFDIIGDVHGCIGELRALLTTLGYAPDGPGADAVWRHP
                     AGRRAVFLGDLVDRGPDTPAVLRLVMGMVAAGTALAVPGNHDVKLVRKLKGKNVQITH
                     GLAESLAQLDAESPEFRERVATFLDNLVSHYVLDDGKLVVAHAGLPAAMQGRGSGRVR
                     EFALYGETTGETDEFGLPVRYNWAAGYTGKAMVVYGHTPVPAPEWLNRTINIDTGCVF
                     GGRLTALRYPEEELISVPAAEIYAPPVRPFLPAEPADAAAPALTAQQQHDDVLDIADV
                     LGKRLITTRLQPNIMIPEAQATAALEVMSRFAANPQWLIYLPPTMSPSETTQEPGLLE
                     HPAEAFAYYRQAGVPRVVCEEKHMGSRAVVIVCRDAETAHRRFGVTTGEIGICYTRTG
                     RRFFDDPALEAAFLVRVRDALDATDLWTTLRTTWVCLDCELMPWSAKAQELLRRQYAA
                     VGAAAGAAFAAALPALAGAAAWEEDAAPLLARYDARAAMLDDYVAAYRRYCWPVHALA
                     DLKLAPFHLLAGEGAVHTDKDHLWHMAHLAPLAAAAPDLLLATAHQVVDVTDAASVEA
                     GVAWWQALTGRGGEGMVVKPLDFIARGPQGLLQPAVKCRGPEYLRIIYGPEYLAPAHL
                     ARLRARGLGRKRSLALREFALGLEALERFVRREPLRRTHECVFGVLALESEPVDPRL"
BASE COUNT          713 a         1783 c         1699 g          735 t
ORIGIN      
        1 gcacgcgcct gccggagcgg cccgattacg cccgcgccga cgccctgctc atccgggcgc
       61 gccgggcgat ggtgggagat gactaagatg ctgccggtgc tggacccgcg tgtgcgggcc
      121 gaagttctcg cgcaacccta ctcgctgctg ttcgtgacca tcagcggcgc gcatctctac
      181 ggcttcccgt cgcccgactc ggactacgat ctgcgcggcg cacacatcct gcccttgccg
      241 gacgtggtgg ggctggagcc gggccagaac acggtgacgg tctcggcggt gcgtgagggg
      301 ctggaggtgg acctggtcag ccacgacgtg cataagttct tcacgctgat gctgaagcgc
      361 aacggctacg tgctggagca gctttactcg ccgctggtgg tccacaccac gccggaacac
      421 gccgaactcc gcgccatcgc gcggggctgc atcacccggc accacagcca ccactaccgg
      481 ggcttcgcgg ccacccagtg ggggctgttc gagaaagagc gcccgcgccg ggtcaagccg
      541 ctgctctacg tctaccgcgt gctgctcacc ggcatccacc tgatgcgcac gggcgaggtc
      601 gaggccaacc tgctgcggct gaacgagacc ttccgcctgc cctacatcgc cgacctggtg
      661 gcgcgcaagc tggccgggcc ggagcacggc acgctggacg ccgtggatgt ggctttccat
      721 gcccaggagt accagcggct gtgggcgctg ctggaagagg cgcacctggc gagcaccttg
      781 cccgaagcgc cctcggcccg ccccgccctg cacgatctgc tggtgcgctt gcggcttggc
      841 gcgtgatgct gtatatcctt atacaagggc gtaactagcc cgcgatcttt tgggagggtg
      901 cttgcatgac tcggcccgta tccacaaccg aaaaccggcg ctgggggcgg gggtgcctgc
      961 tggcggggct gctgctggcg gcgtgcgcgg acagcggtcc cgctcccacg cccaccgcgg
     1021 tcgccgtcgg cggcaacgct gccctggcca ccgcgatccc agcgaccagc actgccattc
     1081 ccatcccggc cactccaacg atcttcttgc tgccggatgc gcggacaaaa ctggcggccg
     1141 tcaagaccgt tgatcccgct gcctacgcga ccttttacgc tagggaagtc gctcgcgaac
     1201 agcgcatgct gacgccccgt ccgagcaata ccccgcagcc caccccgctg cccattaccg
     1261 ccaccccgca gttgggcttg tggactgact gcgggcacgg ggtccatggc gatcccctgc
     1321 ccgctaactg ctggcaactc tttatcaacg gcaccttata tcgggtcatg gcgggccggc
     1381 tcgaacccga gtccgaggcc gatcaaggcg tgctgtgggt gaagcgcctc gatcagcgta
     1441 gtcaggacgt gccctggtat ctcaccccgc agcgggtcgg ccccgtcagt atcgtggctg
     1501 tggatgggct gcgcttcacc gtggcgaccg atgacccgcc gaacccgccg gtcagctttg
     1561 tgttcgacct ggcgacgcgc cagtggctgc acccggacgg cacgccctgg cccacgccca
     1621 cgccgctgcc caccatgccg ccgtgatgcg gggccggggg ggcgcgtgat tccgtgcgcg
     1681 agcccggtgc cttgacataa ttcgcgaaga ggagcaagga tgtggacgat ctgttgcaag
     1741 aagtgcggac cgtgttgatc accaccgccg cccgctggct cagtatgacg gcgacgcttt
     1801 cgcccgccgc gctggcgcgc ccgccggccc ccggcgagtg gtccgccgcc gaatgcctgg
     1861 ggcacctgat ccagaccgag cgggacgtgt tcccgcgccg ggtgcgtaac ttcctggccg
     1921 gcgaggatct ctccgggttc acgccgtccg gcgccacggg cgacgcggac ctcagcctgc
     1981 gggatctggc cgccgagttc gcccgcctgc gcgccgccaa cctggagctg atcgcggaca
     2041 tccaaccggc ggacctgggg cggagcgccc ggcaccccga gctaggcccg gtgacgctgg
     2101 gcgaggtgct gcacacctgg gccgcgcacg acctgaacca cacgatgcag gccgaacgcg
     2161 ccctgatgca accgttcatc gccgggtcgg gcgcgtttcg cgtgcggttc gcggagcatg
     2221 tgctgcccaa gcaggcctga ttcttcaccg cgaaggaacg aaggaaacga agaataagac
     2281 ctatataaaa taaccttcgc gttcttcgtt ccttcgcggt gaactagttc gtcgcggaga
     2341 ccttgcgttt agaaagccaa aacatgaaca tcaccatccc ggaactggcc ctcgtcgtgc
     2401 tcatcggtgc gtcgggggcg gggaaatcga ccttcgcgcg gacccacttc cggcccaccg
     2461 agattctgtc gtcggatttc tgccgcgggc tggtggccga cgacgagaac gaccagtcgg
     2521 ccacgaccga cgccttcgac gtgctgcact atatcgcggc caagcggctg gccgccgggc
     2581 ggctgaccgt ggtggacgcg accagcgtgc agccggaggc gcgcaagccg ctggtggacc
     2641 tggcccgcga gtaccactgc ctgcccgtcg ccatcgtgtt caacctgccg gagcggctct
     2701 gccacgagcg caaccagggg cggcccgacc gcgccttcgg cccgcacgtg gtgcgccgcc
     2761 aggtgcagca gttgcaccgg tcgctgcgcg ggctgcgcgg cgagggcttc cgccacgtct
     2821 tcgtgctcga cacgccggag gaggtcgccg ccgtcacggt ggagcgccag ccgctctgga
     2881 acaaccggcg caccgagcac ggcccgttcg acatcatcgg cgacgtgcac ggctgcatcg
     2941 gcgaactgcg cgcgctgctc accacgctgg gctacgcgcc cgatgggccg ggggcggacg
     3001 ccgtgtggcg gcaccccgcc gggcgccggg ccgtcttcct gggcgacctg gtggaccgcg
     3061 ggccggacac gcccgcggtg ctgcgcctgg tcatgggcat ggtggcggcg gggacggcgc
     3121 tggccgtgcc cggcaaccac gatgtcaagc tggtgcgcaa gctgaagggc aagaacgtcc
     3181 agatcaccca cgggctggcc gagtcgctgg cccagttgga cgccgagtcg ccggagttcc
     3241 gcgagcgcgt ggcgaccttc ctcgacaacc tggtcagcca ctacgtgctc gacgacggca
     3301 agctggtggt ggcccacgcc gggctgcccg ccgccatgca ggggcgcggg tcgggccgcg
     3361 tgcgcgagtt cgcgctctac ggcgagacca ccggagagac cgacgagttc ggcctgccgg
     3421 tgcgctacaa ctgggccgcc ggctacaccg gcaaggcgat ggtggtctac ggccacacgc
     3481 cggtgcccgc gcccgaatgg ctcaaccgca ccatcaacat cgacacgggc tgcgtgttcg
     3541 gcgggcggct gaccgcgctg cgctacccgg aggaggagct gatctcggtc ccggcggcgg
     3601 agatctacgc gccgcccgtg cgcccgttcc tgcccgccga gcctgcggac gcggcggcgc
     3661 ccgcgctcac cgcccagcag cagcacgacg atgtgctcga catcgccgac gtgctgggca
     3721 agcggctgat caccacgcgc ttgcagccca atatcatgat ccccgaagcg caggccaccg
     3781 ccgcgctgga ggtgatgagc cggttcgcgg ccaacccgca atggctgatc tacctgccgc
     3841 ccaccatgtc gccgtcggag acgacgcagg agccgggcct gctggagcac cccgccgagg
     3901 cgttcgccta ctaccggcag gcgggcgtgc cccgcgtcgt ctgcgaggag aagcacatgg
     3961 ggtcgcgcgc cgtggtcatc gtctgccgcg acgcggagac ggcgcaccgg cgcttcggcg
     4021 tgaccaccgg cgagatcggc atctgctaca cgcgcaccgg gcgccgcttc ttcgacgacc
     4081 cggcgctgga ggccgccttc ctggttcgcg tccgcgacgc gctcgacgcg accgatctct
     4141 ggaccacgtt gcgcacgacc tgggtctgcc tcgattgcga actgatgccc tggtcggcca
     4201 aagcgcagga gctgttgcgc cgccagtacg ccgcggtggg cgcggcggcg ggcgccgcct
     4261 tcgccgccgc gctgcccgcg ctggccgggg ccgccgcctg ggaggaggat gccgccccgc
     4321 tgctggcccg ctacgatgcg cgcgccgcca tgctcgacga ctacgtggcc gcctaccgcc
     4381 gctactgctg gccggtccac gcgctggccg acctcaagct ggccccgttc cacctgctcg
     4441 ccggcgaggg cgccgtgcat accgacaagg accacctctg gcacatggcg cacctggcgc
     4501 cgctggccgc cgccgcgccc gacctgctgc tggcgaccgc gcaccaggtg gtggacgtga
     4561 ccgacgcggc cagcgtggag gcgggcgtgg cctggtggca ggcgctgacc gggcgcggcg
     4621 gcgagggcat ggtcgtcaag ccgctcgact tcatcgcccg cgggccgcag gggctgttgc
     4681 aacccgccgt caagtgccgc ggaccggaat acctgcgcat catctacggg ccggagtacc
     4741 tggcgcccgc ccacctggcc cggctgcggg cgcgcgggct gggccgcaag cggtcgctgg
     4801 ccctgcgcga gttcgccctg ggcctggagg cgctggagcg cttcgtccgc cgcgaaccgc
     4861 tgcgccgcac ccacgagtgc gtcttcggcg tcctggccct cgaaagcgag ccggtcgatc
     4921 cgcgcctgta
//
LOCUS       DASUYC010001056         3662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_2028429, whole genome shotgun sequence.
ACCESSION   DASUYC010001056 DASUYC010000000
VERSION     DASUYC010001056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2028429"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="VF276_17265"
     CDS             <1..686
                     /locus_tag="VF276_17265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6608652.1"
                     /translation="LRRDADLIFTTGAPLREFLGAGRDRVWFVPNGVDPAQFQPHAPH
                     PIPVDMRALPRPIVGYSGKMQQRIDIELMQAVAAALPDVSFVFIGQILNRAWMEPLYR
                     VPNIHFLGDKPYAELPDYLAAFDVCIIPHHVGKYEHGGTALKLYEYMAMHKPIVTTGI
                     GGVDMFADRICIANSAEAFAGGIRRYLAALQAHEPIPGIADPLPPGNTWQEKAAFILD
                     RIAERWPAV"
     gene            683..1465
                     /locus_tag="VF276_17270"
     CDS             683..1465
                     /locus_tag="VF276_17270"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX6608653.1"
                     /translation="MSASTARGGLLHRLASRVASRLKGGEAYRLDPAMSPRMLADVTA
                     RRGFAALRGLRFRLRFGASRGLIFAGHNVRIHHAYQIRAGRSLILEDGVYIDALSRQG
                     VRFGNNVTIAKYAVIQVTGVAQNLGVGLRLGNHVGLGAFNFIGAQGGVSIGDNVICGP
                     RVSFHAENHVYSDPGTPIRLQGVTRRGIVVEDDCWIGAGAMVLDGVRIGRGTVVAAGA
                     VVTRDVPPYSVVAGVPARVIKSRRPEQPGVLGEKNLTTTPEF"
     gene            complement(1478..>3662)
                     /locus_tag="VF276_17275"
     CDS             complement(1478..>3662)
                     /locus_tag="VF276_17275"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX6608654.1"
                     /translation="LLIGIFLAQTLVVCAAGPDRWAPILGGLVALNWLLLPLVLIGTD
                     WAGKASLSGAAAYAQLPSWLPHFFTNSYGQAPDGIQPNQLAIMLALLLPLSLTLIRTP
                     PDSPLRVVIGTGRLARLNQGLLRKRGLIVLAALTLGAILFTQSRGGLLACGVALVVLG
                     GWRYRRYLGWAVLGLLMVAALLLVLPGVRDSSAWLFTPQGPQAHLSNTLSIRVELWQR
                     AVRMIKDYPFTGIGLNTFPEVQTTLYPLVSVHRTEFALPTAHNLYLQALLDFGIPGGL
                     VFFALLLTLARGIRRAAQFGPGPGDLAPTIAAAGAAWLTFGLLDVLDLSSKGGYILWV
                     YIGLLALLVQAQGAAAARRGWRGFRQMARGPRLALAGVVGAGLLGLVLSGPYLVAAAY
                     LNWGSIQVSKHRDALANAAPPAEAAFAASRAWWPGSSGALYQQGRVRLAAGNYARAVA
                     ALEPAHALSPRDSLISYLLGQAYQGAGMPAQAAQAWHEANSWFLSFPPLVSEGLQAVN
                     QRQTAQADTRFQQAQVLAPDIDPDHQEWRANLYYGLALVARQQGRPAEAKAQFDAALA
                     AGPHNMAVLVEAGTFYATALGQVDQGLALVELAARENPQSYWCAMKQADLYNQLQRRG
                     EAMAAWRRAIPLAPEGDTTADRALAGAYLVADQPAAAVAVLTPAWARVPADGEVAYLL
                     GTAQLGAGNRVAGCATLGEAAGLSPGAAYHQALADKLRTCTQAAP"
BASE COUNT          588 a         1330 c         1229 g          515 t
ORIGIN      
        1 tgctccgccg cgacgccgac ctgatcttca ccaccggagc gccgctccgc gaattcctgg
       61 gcgccgggcg cgaccgggtc tggtttgtgc ccaacggcgt cgatcccgcg cagttccagc
      121 cccatgcgcc gcacccgatt cccgtcgata tgcgcgcgct gccccgcccg atcgtgggct
      181 attccggcaa aatgcaacag cgcatcgata tcgaattgat gcaggccgtg gccgccgcgc
      241 tcccggatgt cagctttgtg ttcatcggcc agatcctcaa tcgcgcctgg atggaaccgc
      301 tgtatcgtgt ccccaacatc cacttcctgg gcgataaacc ctacgccgag ttacccgact
      361 acctggccgc gtttgacgtg tgtattatcc cgcaccacgt gggcaaatat gagcacggcg
      421 gcaccgcgct caagctctat gagtacatgg cgatgcacaa gcccatcgtg acgaccggca
      481 tcggcggcgt ggatatgttt gccgaccgga tttgcatcgc caacagcgcg gaggcgttcg
      541 ccggcgggat ccgtcgctac ctggcagcgt tgcaagccca cgagccgatc cccggcatcg
      601 ccgacccgct gccgcccggc aacacctggc aggagaaggc cgccttcatc ctcgaccgga
      661 tcgccgaaag atggcccgcc gtatgagcgc gtccacggcg cgcggcgggt tgctgcaccg
      721 gctcgcctcc cgcgtggcct cccggctgaa gggcggtgaa gcctaccgcc tggacccggc
      781 catgagtccg cgcatgctgg ccgatgtcac ggcgcggcgg ggctttgccg ccctgcgcgg
      841 gctgcgcttc cgcctgcggt tcggcgccag ccggggcctg atcttcgcgg gccacaacgt
      901 gcgcatccac cacgcctacc agatccgcgc cgggcgcagc ctgatcctgg aggacggcgt
      961 ctacatcgat gccctgtcgc ggcaaggggt ccgcttcggc aacaacgtca ccatcgccaa
     1021 atacgccgtc atccaggtca cgggcgtggc gcagaatctg ggcgtgggcc tgcgcctcgg
     1081 caatcacgtg ggcctgggcg cgttcaactt tatcggcgcc cagggcggcg tgtcgattgg
     1141 cgacaacgtg atctgcgggc cgcgggtcag cttccatgcc gagaaccacg tctatagcga
     1201 ccccggcacg ccgatccgcc tgcagggtgt cacccggcgg ggcatcgtgg tcgaggacga
     1261 ttgctggatc ggcgccgggg cgatggttct ggacggcgtg cgcatcgggc gcggcacggt
     1321 ggtggccgcg ggcgcggtcg tcacgcgcga cgtgccgccc tacagcgtcg tggccggggt
     1381 gccggcgcgg gtcatcaaga gccgccgtcc tgagcaaccc ggcgtgctcg gcgagaaaaa
     1441 cctcacgacc acacccgagt tctagggcgc ccaacgatca aggggcggcc tgggtacagg
     1501 tccgcagctt atcggccaac gcctggtgat aggccgcgcc gggcgagagg cctgccgctt
     1561 cgcccagggt cgcgcagccg gccacgcggt tccccgcccc cagttgcgcc gtgcccagca
     1621 ggtacgcaac ctcgccgtcg gcagggacac gcgcccacgc cggggtgagc accgccaccg
     1681 ctgcggccgg ctgatcggcc accaggtagg cccccgccag cgcgcgatcc gcggtcgtat
     1741 cgccctcggg cgccagtggg atggcccgcc gccaggccgc catcgcctcg cctctgcgct
     1801 gcaactggtt atagagatcg gcctgcttca tcgcgcacca gtacgattgc gggttctccc
     1861 gcgcggccag ctcgaccaga gccagcccct ggtccacctg gcccagcgcc gtggcgtaga
     1921 acgtgcccgc ttcgaccagc acggccatat tatgcggccc ggccgccagc gccgcgtcaa
     1981 actgcgcctt cgcttcggcg ggccggccct gctgccgggc caccagcgcc aacccataat
     2041 acaggttcgc ccgccactcc tgatggtcgg ggtcgatgtc gggcgccagc acctgggcct
     2101 gctggaaccg cgtgtccgcc tgcgccgtct gccgctggtt cacggcctgc aacccctcgc
     2161 tgaccagcgg cgggaatgac aggaaccacg aattcgcctc gtgccacgcc tgggccgcct
     2221 gagcgggcat cccggcgcct tgataggcct ggcccagcag gtagctgatc aggctgtcgc
     2281 ggggcgacaa cgcatgggcc ggttccaggg cggccacggc gcgcgcatag ttccccgccg
     2341 ccagccgcac ccggccctgc tggtagagcg cgccgctgct ccccggccac caggcccggc
     2401 tcgcggcgaa ggccgcctcc gccgggggcg cggcgttcgc cagggcatca cgatgcttgc
     2461 tgacctggat gctgccccag ttcaagtagg cggcggccac gagatatggc ccgctcagca
     2521 cgaggccgag caaccccgcg cccaccaccc cggccagggc caggcgcggc ccgcgcgcca
     2581 tctgccggaa cccacgccat ccccggcggg ccgctgctgc cccttgcgcc tgcaccagca
     2641 gcgccagcaa cccgatgtac acccatagga tatagcctcc cttggaactc aggtcgagca
     2701 cgtcgagcaa gccgaaggtc agccaggccg cgcccgccgc cgcaatggtc ggggccagat
     2761 cgccggggcc gggtccgaac tgcgccgcgc ggcgaatgcc gcgcgccagg gtcagcaaga
     2821 gcgcgaagaa caccagcccg ccagggatac cgaaatcgag cagggcttgc aggtacaggt
     2881 tgtgcgccgt cggcagggca aactcggtgc ggtggaccga caccagcggg tacagggtag
     2941 tctggacttc ggggaaggtg ttgagtccga tgcccgtgaa gggatagtcc ttgatcatgc
     3001 ggacggcgcg ctgccacaac tccacgcgaa tcgacagggt gttgctgagg tgcgcctgcg
     3061 gcccctgcgg cgtgaacaac caggcggagc tatcgcgcac gccgggcagc accagcagca
     3121 aggcggcgac catgaggagg cccaggaccg cccatcccag gtagcggcgg tagcgccagc
     3181 cccccagcac caccagcgcc acgccgcacg ccagcagtcc cccgcgtgac tgggtgaaga
     3241 ggatcgcgcc gagggtgagc gcggccagca cgatcaagcc ccgcttgcgt agcaacccct
     3301 ggttcagccg ggccaggcgc ccggtgccga tcaccacgcg cagaggcgag tccggcgggg
     3361 tacggatcag ggtcaggctc agcggcagca acagcgccag catgatcgcc agttggttcg
     3421 gctggatgcc gtccggggcc tgcccatagg agttggtgaa gaaatgcggc aaccaactcg
     3481 gcagttgggc ataggcggcg gcgccgctca gcgaggcctt ccccgcccag tccgtgccga
     3541 tgagcaccag cggcagcagc agccagttca gggcgaccaa cccgcccagg atcggcgccc
     3601 agcgatcggg gccggcggcg cacaccacca gggtctgtgc caggaagatg ccgatcagga
     3661 ga
//
LOCUS       DASUYC010001057         2647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_411736,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001057 DASUYC010000000
VERSION     DASUYC010001057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411736"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="VF276_17280"
     CDS             complement(<1..448)
                     /locus_tag="VF276_17280"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX6608655.1"
                     /translation="MPDLEMLLQRLEAQGYKRTAPRRRLLAEIAQRPGPFTAAEIYAA
                     LQSQDAGIGRATVFRTLDLLADLGLLQRMHDEQIGGAGAAYLACEPVHHHHLICSSCH
                     AVWDFVEGDLEVSIAAVARRNAFQMLDHRLELYGVCAACREQAPAGR"
     gene            970..1875
                     /locus_tag="VF276_17285"
     CDS             970..1875
                     /locus_tag="VF276_17285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004891988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX6608656.1"
                     /translation="MRLVTYHEVDELRLGVLHGDQVIDVQRWLSPPSEPLPTTMIGLI
                     AMGETGLQRLRQALDLLPEHALAGAPRLDALKPAAPIPRPRKNVFCIGQNYAAHAAEQ
                     NQAPPARPSVFTKAPTAVTGPYDPILLDPSVSDKMDWEVELGVVIGEGGRGIKREQAL
                     DHVFGYTVINDVSARDIQYAHGGQFFLGKSLDTACPMGPWIVTRDQIADPQQLRLTCR
                     VNGETRQDSDTSDMIFTVAALIEWLSRGMTLEPGDLIATGTPSGVGHFRKPPIYLQPG
                     DLVEAEVEGVGTLRNRVELWPGGPR"
     gene            1854..2501
                     /locus_tag="VF276_17290"
     CDS             1854..2501
                     /locus_tag="VF276_17290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608657.1"
                     /translation="MARRPALAVATGLALLLVLWGLVGAGALRGLDGPVHAWTLRHRP
                     PGSGTLIKWLLAGEMLVLAGVLAWLLRGHLGLLARLGALVIGAEGVVWALKHAGRGWD
                     VPWYANNFPSGHTTVGALLCLLLARVLAALPGPDAGLVGMLRGVCFALAGLFGALAVF
                     PMGHVPSEMVGGFVLAGTAAQIGGGWIAAALPPRALVPVDSAGRLLHTGEGRRRV"
BASE COUNT          389 a          832 c          985 g          441 t
ORIGIN      
        1 cccggccggc gggcgcctgc tcccggcagg cggcgcagac cccatagagt tcgagccgat
       61 gatccagcat ctggaaggcg ttgcgccggg ccactgcggc gatggacact tccaaatcgc
      121 cctcgacaaa gtcccagacg gcgtggcacg acgaacagat gagatggtgg tgatggaccg
      181 gctcgcaggc caggtaggcc gccccggccc cgccgatctg ctcgtcgtgc atgcgctgga
      241 gcaggccaag gtcggccagc aggtcgagcg tgcggaagac ggtggcccgc ccgatgcccg
      301 catcctggct ttgcagggcg gcgtagatct cggcggcggt gaacgggccg ggccgctgag
      361 cgatctcggc cagcagccgg cggcgtggcg cggtgcgttt gtagccctgg gcttctaacc
      421 gttggagcag catttccagg tcaggcatgg ctatacgatc ctctgttcgt gcgctaactg
      481 atatccagta tcatctacgc cattctagca cagttcggca ggcttgtcaa tgggagcggg
      541 cttagttgca ccgcgaaggc gcgaaggacg cgaaggtttt ttgtattggt tgtgttttgt
      601 atggcgcgga tcgtcgtcat ggcggcaacg cccggcccgt gaacaggcgg gctacgtcaa
      661 cgaagcccgc ctgcgcgggc tggcacaatc gggcggagaa cgtccgcgcg ctagcgagat
      721 cgcccggccc gtgaacgggc gggctacgtc aacaaagccc gcctgcgcgg gctgggcgaa
      781 tctagcgaga ggccgcgcag gcggccttgg taggggtagc cggaggcttg agcctccgtg
      841 ctaccgcctc cgtgagcggc ggcggggtat ttcggctata atggcgagtg gtgcggtgct
      901 ggcgcgcttt gtgctgctgg atcgcggggg cgccgcttgg cgctcactac ctcaagcagg
      961 agatttccga tgcgtttggt gacatatcat gaagtggacg aattgcgcct gggcgtgctg
     1021 catggcgacc aggtgatcga tgtgcagcgc tggctcagcc cgcccagcga gccgctgccc
     1081 acgacgatga tcgggctgat cgccatgggc gagacagggc tgcaacggct gcggcaggcg
     1141 ctcgacctgc tgccggagca cgcgctggcc ggcgcgccgc gcctggatgc gctgaagccg
     1201 gcggcgccga tcccgcgccc gcgcaagaac gtgttctgta tcggccagaa ctacgcggcg
     1261 cacgcggcgg agcagaacca ggcgcccccg gcgcggccct ccgtgttcac caaggcgccc
     1321 acggcggtga cgggcccata cgaccccatc ttgctggacc cgtcggtgtc cgacaagatg
     1381 gactgggagg tcgagctagg cgtagtgatc ggcgagggcg ggcgcggcat caagcgggag
     1441 caggcgctgg accacgtctt cggttacacg gtaatcaacg acgtgtcggc ccgcgacatc
     1501 cagtacgccc acggcggcca gttcttcctg ggcaagagcc tggacaccgc gtgcccgatg
     1561 gggccgtgga tcgtgacgcg cgatcagatc gccgacccgc agcagttgcg cctgacctgc
     1621 cgggtgaacg gcgagacccg gcaggactcc gacacctcgg acatgatctt cacggtcgcg
     1681 gcgctgatcg agtggctgtc gcgcggcatg acgctggagc cgggcgacct catcgccacg
     1741 gggacgccga gcggcgtggg gcatttccgc aagccgccca tctacctgca gcccggcgat
     1801 ctggtggagg ccgaagtcga gggcgtgggc acgctgcgca accgggtgga actgtggccc
     1861 ggcggcccgc gctagccgtc gccaccggac tcgcgctgct gctggtgtta tgggggctgg
     1921 tgggcgcggg ggcgctgcgc ggcctggatg ggccggtcca tgcctggacg ctgcgccacc
     1981 gcccgcccgg cagcggcacg ctgatcaagt ggctgctggc cggggagatg ctggtgctgg
     2041 cgggggtgct ggcctggctg ctgcgcgggc acctggggct gttggcccgc ctgggcgcgc
     2101 tggtgatcgg cgctgagggc gtggtctggg cgctcaagca cgcgggccgc ggctgggacg
     2161 tgccctggta cgccaataac tttcccagcg gccacacgac ggtgggcgcg ctgctctgcc
     2221 tgctgctggc gcgcgtcctg gccgcgctgc cgggaccaga tgccggcctg gtggggatgc
     2281 tgcggggcgt gtgtttcgcg ctggccgggc tgtttggggc gctggccgtg ttccccatgg
     2341 gccacgtgcc gagcgagatg gtgggcggct tcgtgctggc aggcaccgcg gcgcagatcg
     2401 gcggcggctg gatcgccgcc gcgttgccgc cgcgggccct ggtgccggtt gacagcgcgg
     2461 ggcgcctttt gcataccggc gagggccggc gacgggtctg aaattgacac aacgctctgt
     2521 catgctgaac gcagtgaagc atctccgtct tcgatcaccg agacgagact cttcgctgcg
     2581 ttcagagtga caatttgggc acaacgctct gtcatgctga acgcagtgaa gcatctccgt
     2641 cttcgat
//
LOCUS       DASUYC010001058         2482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1334328, whole genome shotgun sequence.
ACCESSION   DASUYC010001058 DASUYC010000000
VERSION     DASUYC010001058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334328"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..1629
                     /locus_tag="VF276_17295"
     CDS             115..1629
                     /locus_tag="VF276_17295"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX6608658.1"
                     /translation="MLDFIRGNKINARWPANTPTGGTLDKDEAQVADKLEEPIVRRKT
                     IGLRPGERKLLQAITDWVVTLSGAFVIGLQSGRVYHGTEVVLSAAILAALWFFFANAF
                     DAYDITVLQNHFRSAYKGGKVFLVTGTAYLLVAGFTGGTLPVIRPRISESLLALFLVG
                     PVIMILRTVAAQVLNHAPLRRRVVVVGANESGYEMVQALSHYGGRTYEFLGYFDDQPA
                     EPMAGDDGPPPTAQPIADLLRLNNEQGIDQIVLANPEQTPELLRALSICHERGIQITP
                     MFALYQELTGRVPVSHLGRDWFVALPAHVKNTARTYLVLKRLVDLTLGLVLMTLTAPL
                     IPIIALAIKLDSPGPVFFKQVRAGRGGKPFKIVKFRSMRQDAEAGTGAVWASSADSRI
                     TRVGRIMRGTRLDELPQLWNVVVGDMSFVGPRPERPELEERLEREIPFYRARRAVRPG
                     LTGWAQVRYGYGNTMLDALRKVEYDLYYIKNESLYLDLLIMLRTVSVIIRRSGT"
     gene            complement(1607..>2482)
                     /locus_tag="VF276_17300"
     CDS             complement(1607..>2482)
                     /locus_tag="VF276_17300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6608659.1"
                     /translation="VMGRVAGFLTRRPVVSTIHNNRVDLDEEPARRQWMERVTARWMG
                     RRLIVVAENLREEIAAWFGVPLDKVVAITNGVDTDRFHRDGSFDRAAVRRALAGGDFP
                     LATSVGRLVPQKAQHILIEAAAQVAAQRPDTRVVLLGEGPLAEELAAQAAKTGVQDQV
                     IFSGFRNDVADILAASQIFVLSSAWEGLPIALLEAMAAGCAAVCTDVGGVSQVLEHGV
                     TGLLVPPNDPAALAAALLACFNDPARTAQMGEAGRIAVVERYSMAAWVRRCQALYLQE
                     VAPAPRRRIKSRSAG"
BASE COUNT          408 a          871 c          809 g          394 t
ORIGIN      
        1 cgaatccacg gattcgtggc gcaagttgga tagcggccgg aacatcgtca tccagccggc
       61 agactcgccg cacgccacga aatgcagcgc aagaaaggcg gagtggtagg atatatgttg
      121 gactttatcc ggggtaataa gatcaatgcc cgttggccgg cgaatacccc aaccggggga
      181 accctggaca aggacgaggc gcaggttgcc gacaagctgg aggagcccat cgtgcgccgc
      241 aagacgatcg gcctgcggcc cggtgagcgc aagctgttac aggcgatcac cgactgggtc
      301 gtcactctca gcggagcctt cgtcatcggg ctgcagagcg ggcgggtcta tcatggcacc
      361 gaagtggtgc tcagcgccgc catcctggcc gcgctctggt tcttcttcgc caacgccttc
      421 gacgcctacg acatcaccgt gctgcaaaac cacttccgca gcgcctacaa aggcggcaag
      481 gtgttcctgg tcaccggcac cgcctacttg ctggtcgccg gcttcaccgg cggcaccttg
      541 ccggtgatcc ggccgcgcat cagcgaatcc ttgctggcgc tgttcctggt cggcccggtg
      601 atcatgatcc tgcgcaccgt cgcggcccag gtgctcaacc acgcgccgct gcgccggcgg
      661 gtcgtcgtcg tcggggccaa cgagtcgggc tacgaaatgg tgcaggccct gtcgcactac
      721 ggcgggcgca cctacgagtt cctgggttac tttgacgatc agccggcgga gccgatggcc
      781 ggggacgacg ggccgccgcc caccgcccag cccatcgccg atctgctgcg cctcaacaac
      841 gaacagggca tcgaccagat cgtgctggcc aacccggagc aaacgcccga actgctgcgc
      901 gcgctctcga tctgccacga gcgcggcatc cagatcacgc cgatgtttgc gctctaccag
      961 gagctgaccg ggcgcgtgcc cgtctcgcat ctgggccgcg actggtttgt ggccttgccg
     1021 gcccacgtca agaacaccgc ccgcacctac ctggtgctca agcgcctggt ggacctgacc
     1081 ctgggcctgg tgctcatgac gctcaccgcg ccgctgatcc cgatcatcgc gctggcgatc
     1141 aagcttgact cgcccggccc cgtcttcttc aagcaggtgc gcgcgggccg gggcggcaaa
     1201 cctttcaaga ttgtgaagtt tcgctcgatg cggcaggatg ccgaagcggg gacgggcgcc
     1261 gtctgggcca gcagcgcgga ctcgcgcatc acgcgcgtgg ggcgcatcat gcgcggcacc
     1321 cggctcgacg agctacctca gttgtggaac gtggtcgtcg gcgacatgag cttcgtcggc
     1381 ccgcggcctg agcgacccga gctggaagag cggctggagc gggaaatccc gttctaccgg
     1441 gcccggcggg cggtgcgccc tgggttgacg gggtgggccc aggtgcgtta cgggtacggc
     1501 aacacgatgc tcgacgcact gcgcaaagtc gagtacgatc tctactacat caaaaacgaa
     1561 tcgctctatc tcgacctgct aatcatgctg cgcaccgtct cggtgattat ccggcggagc
     1621 gggacttgat ccggcgccgg ggcgcgggcg cgacctcctg caggtacagc gcctggcacc
     1681 ggcgcaccca ggccgccata ctgtatcgtt cgaccaccgc gatccggccc gcttctccca
     1741 tctgggcggt gcgggccgga tcgttgaagc acgccagcag cgcggcggcc agggccgccg
     1801 ggtcgttggg cggcaccagc aacccggtca cgccatgctc caacacctgc gacacgcccc
     1861 ccacatcggt gcagaccgcg gcacacccgg cggccatcgc ctccaacaac gcgatcggca
     1921 gcccttccca ggccgacgac agcacgaata tctggctggc ggccaggatg tcggccacgt
     1981 cgttgcggaa gccgctgaag atcacctggt cctgcacccc ggttttggcc gcctgggccg
     2041 ccaattcctc ggccagcggg ccctcgccca gcagcaccac gcgggtgtcg ggccgttgag
     2101 ccgcaacctg ggccgccgcc tcgatcagga tgtgctgggc cttttgcggc accaggcgcc
     2161 cgacgctggt cgccagcggg aagtcgcccc cggcgagcgc gcgccgcacc gccgcccggt
     2221 cgaaactgcc gtcccggtgg aagcggtcgg tatcgacccc gttggtgatc gccaccactt
     2281 tgtcgagcgg cacgccgaac caggcggcga tctcttcgcg caggttctcg gccaccacga
     2341 tcaggcggcg ccccatccag cgcgccgtca cgcgctccat ccactggcgg cgggccggct
     2401 cttcgtccag gtccacgcgg ttgttgtgga tggtggacac aacaggacgg cgggtgagga
     2461 agccggccac ccggcccatc ac
//
LOCUS       DASUYC010001059         2164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1798327, whole genome shotgun sequence.
ACCESSION   DASUYC010001059 DASUYC010000000
VERSION     DASUYC010001059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1798327"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            276..1361
                     /locus_tag="VF276_17305"
     CDS             276..1361
                     /locus_tag="VF276_17305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX6608660.1"
                     /translation="MNSKMFIWLIGLLGGAAIGWSIAGYLQQASGSVELPLQLAITLA
                     GAFFGLILIPYITSVPLRWLRRRLKLIAAADILAAAIGLILGLVVAALLSIPLSQLPN
                     ELGHLLPSAAAIVFGYLGIYLMVMRRAEFLDILGGMNPVHLAHPPVTEAEKEPAAEKY
                     ILIDTSAIIDGRIADISQTGFLDGVLLIPRFVLNELQRIADSPDTLKRNRGRRGLEML
                     KRLQKDSVIPIQISEIDATDVNDVDGKLIKIGRTYHYPVVTNDYNMNRVAELQGVRVL
                     NINELANSVKPAVLPGEEMRVRVIQEGKELNQGVAYLDDGTMIVVENGRRYVDRGDVD
                     VMVTRVLQTVAGRMIFATVKEHQRESA"
     gene            1358..2080
                     /gene="ispD"
                     /locus_tag="VF276_17310"
     CDS             1358..2080
                     /gene="ispD"
                     /locus_tag="VF276_17310"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEX6608661.1"
                     /translation="MSGPRWCGAIVVAAGGSRRMGGIDKQFALLGGQPVLARSVAAFE
                     TTPEVDAVVVVANPATRDQVQRLGQAAGWRKVVAVVPGGVRRQDSADHGLAALEQAAT
                     AAGATLDLVLVHDGARPLAGRALIERGIAAARTHGAAIAAIPVRDTVKVVDADERITA
                     TPDRARLQQAQTPQVFRYDLLAAAYRAARAQDLSVTDDAALLEALGHPVYVYEGAPSN
                     LKITTPEDLILAEALLAAASPT"
BASE COUNT          352 a          745 c          716 g          351 t
ORIGIN      
        1 gacctccggc tgcagccttt tattttgcgg ggagggtggg ccaggccggc catccgttgt
       61 cgatacccgg cggccatacc agttcctaga cctgcccgct gcccgcgggg cgcggccggt
      121 ctgaggcgga gaacagcgtg cccggcgacc cttgttgagc aaggggccgc cgtttttgtt
      181 gctcacccgg catcgttccg gcacctgctt attcgccggc gccgccgggg tgatcgaccc
      241 gctgtgcggg tccgcactta tctggaggcc gtgaaatgaa tagcaagatg ttcatctggc
      301 tgatcggcct gctcgggggc gccgcaatcg ggtggagcat tgccggctac ctgcaacaag
      361 ccagcggcag tgtggagttg cccttgcaac tcgccattac gctggccggc gccttctttg
      421 gcctgattct gatcccctat atcaccagcg tgccgttgcg ttggctacgg cggcggctga
      481 agctcatcgc cgccgccgac atcctagccg cggccattgg cctgatcctg ggcctggtcg
      541 tggccgcgct gctttccatc ccgctcagcc agttgccgaa cgaactgggg cacctgctgc
      601 cctcggcggc ggccatcgtc ttcggctatc tcggcatcta tctcatggtg atgcggcggg
      661 ccgaattcct cgacatcctc ggcgggatga acccggtgca cctggcccat ccgcccgtga
      721 cggaggcgga aaaggaacct gccgccgaga agtacatctt gatcgacacc agcgccatca
      781 tcgacgggcg catcgccgac atcagccaga ccggcttcct cgacggcgtg ctgctcatcc
      841 cgcgctttgt gctcaacgaa ctccagcgca tcgccgactc gcccgacacg ctgaagcgca
      901 accgcgggcg gcgcgggctg gagatgctca agcggctgca aaaggactcc gtcatcccca
      961 tccagatctc cgagatcgac gctaccgatg tgaacgacgt ggacggcaag ctgatcaaaa
     1021 tcgggcggac gtaccactac ccggtcgtga ccaatgacta caatatgaac cgcgtcgccg
     1081 agctacaggg cgtgcgcgtg ctgaacatca acgagctagc caactcggtg aaacccgctg
     1141 tgttgcccgg cgaggagatg cgcgtgcgcg tgatccagga gggtaaggaa ctcaaccagg
     1201 gcgtggccta cctggacgac gggaccatga tcgtggttga gaacgggcgc cgctatgtgg
     1261 accgcggcga tgtggacgtg atggtcacgc gcgtgctgca gacggtggcc gggcgcatga
     1321 tcttcgcaac cgtgaaggaa caccagcggg agtcggcgtg agcggaccgc gctggtgcgg
     1381 ggccattgtc gtggccgcgg gcggcagccg ccgcatgggc ggcatcgaca aacagttcgc
     1441 gctgctgggc gggcagccgg tgctcgcccg cagcgtggcc gcctttgaaa ccacgccgga
     1501 ggtcgatgcc gtcgtggtcg tcgccaaccc ggccacgcga gatcaggtgc agcgcctggg
     1561 gcaggcggcg ggctggcgca aggtggtcgc cgtggtgccg ggcggcgtcc ggcgccagga
     1621 ctcggcggac cacggcctcg ctgctctcga acaggccgcc accgccgccg gggccaccct
     1681 cgacctggtg ctggtgcacg acggggcgcg cccgctggcc gggcgcgcgc tgatcgagcg
     1741 cggtatcgcc gccgcccgca cccacggcgc ggccatcgcc gccatccccg tgcgcgatac
     1801 cgtgaaggtg gtggacgcag acgagcgcat caccgccacg ccggaccgcg cccgactcca
     1861 gcaagcgcaa acgccgcagg tcttccgcta cgacctgctg gccgcggcct accgcgccgc
     1921 ccgcgcccag gacctgagcg tgaccgatga cgccgccctg ctggaagcgc tcggccaccc
     1981 cgtctacgtc tacgaaggcg ccccatccaa cctcaagatc accaccccgg aagacctgat
     2041 cctggccgaa gctttgctcg ctgctgcctc gcccacctag tccgcctcgc cgcccgcgat
     2101 acggggaaaa gctgccgtgt tccgaggagt gacggcagca tggagtctgg tagacctcta
     2161 gatt
//
LOCUS       DASUYC010001060         3949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1568051, whole genome shotgun sequence.
ACCESSION   DASUYC010001060 DASUYC010000000
VERSION     DASUYC010001060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1568051"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2189
                     /locus_tag="VF276_17315"
     CDS             <1..2189
                     /locus_tag="VF276_17315"
                     /inference="COORDINATES: protein motif:HMM:NF040129.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HEX6608662.1"
                     /translation="AGSMERTRAGFTSILGSAQAADALIKQLQNEANISPFELDSFRK
                     AAQLLLGMGVAADDITPRLHEVSNVVAAVGGSPEAFDRISLALGQIQAKGKVQAQEIN
                     QLAENGVAGWDILAKAMNKSRAEVIGLAEDGKISAETFFNAFAQYANSPAIAQAAEKQ
                     SHTFQGLVSTIHDVGQALETAFGTPLLAAVEPTLERIVDALKDPATVETLAQWGAAAG
                     QFVVGLINAGETAFSVGGQIIQALKPVTDMIGSLLGINIAGPNISAGAFTPPDLSTSV
                     NQAQQFGAGIKSSADSAKSAKDQIADLERQTRELSRATAEANVGYDHRKQSLQDQLTL
                     LDRQYQAENKAADISQLQQKIAKDRALAVDQYSSQGQAAAARLVDEEARLAQLQREQG
                     HDAAKNAIEDRIKAVDREKQANSEASAARTRQLQEQMDALRANAAAVTALTNAIKPPA
                     PDKSKNRNVNDAFGDEQTSSKAQGTIGLLQQLADALTGNKGVKAAIDSVGIAWGQLGD
                     AFKASNPELAQLKTSIDDIAHALGLDAGFAAEAVIAQAAFKQFAETAAALVPQNLAVV
                     GAGLKELNDQLNITAKGLTLFLDKVQGKDDRTIAADLQAFAAALQKADTDRQRGVAGF
                     VQQVNAGNQRIGDITQQNIQDLVNGLIESGKQGTVSSGGATSNAPDFGPRTTGNGPDF
                     GPGVTTTRSRNAALAQQHTIRMEFDERGFKGFVDATTDRRMQEALR"
     gene            2186..>3949
                     /locus_tag="VF276_17320"
     CDS             2186..>3949
                     /locus_tag="VF276_17320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608663.1"
                     /translation="MTARYSVMVGWASTEMHEIAQTGFYDANASTLQRMARDQETASN
                     VRIIFDFNAPGVPPIREWMQIWAGWRNGNVWLQGPDPPFLGFLVQLEGSGAATHVITA
                     QVQTMAYLTSRRTVIGWPTGESDGLPTAGYPADYSVGNWLVGDTPYPGILRFYFPAIL
                     MDGIDPYFDTLTLDATAIPGGNTEALVGQWGFTTVEAVLQDMIGVSRYVAARDDGLLI
                     EPVGWWQTVAQGSILRPRYRLIDANDTASKPVKGVFSVSPAVGEFPFEKFSHTRDGKD
                     VRTDVVVKGIGSDPTTGALIYSRYQGHIADYPCEYLPDGIGGEPYFDTAIDTQAKADA
                     VAATIGYRVWGALGALSWVTDGVAEGGTAITYFEPGDVIALYDPAEGQDYTRYVVRKV
                     TPQGGTAGHPVYRIEVGDVIPNVKELIRGATIVQPHRRAIGTGGGTNTRGAALGTHGL
                     PGAPPQIPMEARPVHSQQNNMRAAFTDVIDLTTDQPTQLRPDVDPDNPPTPAVRQLHR
                     DQYGNPRQWLGPEGADGVHHYTFHVTAAGEYLFTIPDPVTLVEELIQNPDGSVNSDIT
                     TSYLANDSAVPVPSGINPLTCT"
BASE COUNT          807 a         1279 c         1285 g          578 t
ORIGIN      
        1 tggccgggtc gatggaacgc acgcgggcgg gcttcacgtc gatcctcggc agcgcacagg
       61 ccgctgatgc cctgatcaag caactccaga acgaggccaa tatcagcccg tttgaactcg
      121 actcgttccg caaggccgcc caactgctgc tgggcatggg cgtcgccgcc gacgacatca
      181 cgccccggct acatgaggtc agcaacgtcg tagcggcggt gggcggcagc ccggaggcgt
      241 tcgaccgcat atcgctagca ttagggcaaa ttcaagcgaa aggaaaagta caagcccagg
      301 aaattaatca gcttgctgaa aatggggtgg cgggctggga catccttgcg aaggctatga
      361 ataagagtcg ggccgaggtc attgggctgg ctgaggacgg caagatcagc gccgaaacct
      421 tctttaatgc cttcgcccag tacgccaaca gccccgcgat tgcccaggcc gcggaaaagc
      481 agtcgcacac gttccagggg ctggtgagca cgattcacga cgtgggccag gcgctagaga
      541 ccgcgtttgg gacgccgttg ctggccgccg tggaaccgac gctggaacgc attgtagacg
      601 cgctgaaaga ccccgcgacg gtcgaaacgc tggcccaatg gggcgcggcg gcggggcaat
      661 tcgttgtggg cctgatcaac gcgggcgaaa ccgccttcag cgtgggcggg cagattatcc
      721 aggcgctgaa acccgtcacc gacatgatcg ggtcgctgct gggcatcaat atcgccgggc
      781 cgaatatcag cgcgggcgcg tttaccccgc cggacctgag taccagcgtg aaccaggcgc
      841 agcaattcgg cgcgggcatt aagagcagcg ccgatagcgc gaaatccgcc aaagaccaga
      901 tcgccgacct ggagcgccag acccgcgaac tgagccgcgc gaccgccgag gccaatgtcg
      961 gatacgacca tcgcaagcaa tcgctccagg accagttgac gttgctggac cgccagtacc
     1021 aggcggagaa caaggccgcc gacatcagcc aactccagca gaagatcgcc aaagaccgcg
     1081 cgctggccgt cgatcagtac tccagccagg ggcaagcggc ggcggcgcgg ctggtggacg
     1141 aggaagcgcg gctcgcgcag ttgcagcggg agcaagggca cgacgcggcc aagaacgcca
     1201 ttgaggaccg gatcaaggcg gtagaccggg agaagcaggc caatagcgag gccagcgccg
     1261 cccggacccg gcaactgcaa gagcagatgg acgccttgcg cgccaatgcc gccgccgtga
     1321 ccgcgctcac caacgcgatc aagccgcccg cgcccgataa gagcaagaac cgcaacgtca
     1381 acgacgcctt cggcgacgag cagaccagca gcaaggcgca aggcaccatc ggtctgttgc
     1441 agcaactcgc cgacgcgctc accggcaata agggcgtcaa ggcagccatc gattcggtcg
     1501 gtatcgcctg ggggcaattg ggcgacgcct ttaaggccag caaccccgaa ctggcgcaac
     1561 tgaaaaccag cattgacgac attgctcacg ccctcggcct cgatgccggg ttcgcggcgg
     1621 aagcggtcat tgctcaggcc gcgttcaagc aattcgccga aaccgccgcc gcgctcgtgc
     1681 cgcaaaacct cgcggtggtc ggcgcgggcc tgaaggaact caacgaccaa ctcaacatca
     1741 ccgcgaaggg cttgacgctg ttcctggata aggtgcaagg caaagacgac cgcacgattg
     1801 ccgccgacct gcaagccttc gcggcggcgc tgcaaaaggc cgataccgac cggcagcgcg
     1861 gcgtggcggg ctttgtccag caggtcaacg cgggcaacca gcgcatcggg gatattaccc
     1921 agcagaacat ccaggacttg gtgaacggtc tgatcgagtc cggcaagcaa ggcacggtca
     1981 gcagcggcgg ggccacgagc aacgcgccgg actttggccc gcgcacgacc ggcaacgggc
     2041 cggactttgg ccccggcgtc accacgacgc gcagccgcaa cgccgcgctg gcccagcagc
     2101 ataccatccg catggagttt gacgagcgcg gctttaaagg ctttgtggac gcgaccaccg
     2161 accggcggat gcaagaggcg ctccgatgac ggcccgctac tctgtgatgg tcggctgggc
     2221 ctccacggag atgcacgaaa tcgcgcagac cggcttctat gatgccaacg ccagcacgtt
     2281 gcagcgcatg gcccgcgacc aggaaacggc atccaatgtg cggattatct ttgatttcaa
     2341 cgcgcccggt gtgccgccga ttagagaatg gatgcagatc tgggcgggct ggcgcaatgg
     2401 gaacgtctgg ttacaagggc cggacccgcc gtttctcggc tttctggtgc aactggaggg
     2461 cagcggggcc gcgacgcacg tgattaccgc gcaagtgcag accatggcct atctgaccag
     2521 ccggcgcacg gtgatcggct ggcccacggg cgagagcgac gggctgccca cggcgggcta
     2581 tcccgccgat tacagcgtcg gcaactggct cgtaggcgat acgccctacc ctggcatctt
     2641 gcggttctac ttcccggcga tcctcatgga cggcatcgat ccgtatttcg atacgctcac
     2701 cttggatgcc acggccatcc ccggcggcaa cacggaagcc ctggtcggcc agtgggggtt
     2761 tacgaccgta gaagcggttc tccaggacat gatcggggtc tcgcgttatg tggccgcgcg
     2821 ggacgatggg ctgctgattg aacccgtcgg ctggtggcag accgtcgcgc aaggctcgat
     2881 cctccgcccg cgctatcgct tgatcgatgc caacgacacg gcgtccaagc ccgtcaaggg
     2941 cgtgttcagc gtcagcccgg cggtggggga attccccttc gagaagttca gtcacacccg
     3001 cgacggcaag gacgtgcgga cggatgtcgt ggtgaagggc atcggcagcg acccgacgac
     3061 cggcgcgctc atctacagcc gctaccaagg gcacatagcc gattatccct gtgaatacct
     3121 accggacggc atcggcgggg agccgtactt tgataccgcc attgacacgc aggccaaagc
     3181 ggatgccgtc gccgccacta tcggctaccg ggtctggggc gcgctgggcg ccttgtcctg
     3241 ggtgacggac ggcgtcgcgg agggtgggac ggcgatcacc tattttgagc cgggcgacgt
     3301 gatcgcgctg tacgaccccg ccgagggcca ggactatacc cgctatgttg tccgcaaggt
     3361 cacgccgcag ggcggcacgg caggccaccc ggtctaccgg atcgaggtcg gcgacgtgat
     3421 ccccaacgtc aaggaactga tccggggcgc cacgattgtg cagccccacc ggcgggcgat
     3481 tggcaccggg ggcggcacca acacgcgcgg ggcggcgctg ggcacgcacg ggctccccgg
     3541 cgcgccgccg caaatcccga tggaagcccg cccggtgcat agtcagcaaa acaatatgcg
     3601 cgccgcgttt accgatgtca ttgacctgac gaccgaccag ccgacgcaac tgcggcccga
     3661 cgtcgatccc gacaatcccc ctacacccgc cgtccggcag ttgcaccggg accagtacgg
     3721 caacccccgg caatggctgg ggccggaggg tgccgacggc gtgcatcact acacgttcca
     3781 tgtcacggcg gcgggggaat acctgttcac gatcccggac ccggtgacgc tcgtcgaaga
     3841 gttgatccaa aacccggacg gctcggtgaa cagcgacatt acgaccagtt atctcgccaa
     3901 tgatagcgcc gtccccgtgc ccagcggcat caatccccta acgtgtacc
//
LOCUS       DASUYC010001061         2926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_181069,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001061 DASUYC010000000
VERSION     DASUYC010001061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181069"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2131)
                     /locus_tag="VF276_17325"
     CDS             complement(<1..2131)
                     /locus_tag="VF276_17325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6608664.1"
                     /translation="MTEMLPDVSVVICAYTEDRWFDIERAIESIRRQSMPAREIILVV
                     DHNAQLLARARAALPDVLVVENREARGLSGARNSGIEVAQGALVAFLDDDAEADPDWL
                     AYLTGACADPHVLGAGGRVDPLWQVDPPRWFPDEFFWVLGCTYRGLPQESAPVRNLYG
                     GCGCIRRTAFSAVGGFRSGLGRIGTRPLGGEETEFCIRLQQHDPQGIFIYEPRARIRH
                     RIPAQRARWDYFARRCYSEGLSKAAIARLVGTQDGLAAERAYTLRTLPRGVQHGLADS
                     VLRRDPGGLGRAAAIVGGLACTSAGYILGTARQAPAGRGRRHIPVQTLEAPPVPAGPA
                     AVTAVRGPVANVHPPTRDRPLRVLQVTARYFPYMGGVETHVYEVSRRLAASGVDVTVL
                     TSDPTDRLPAQEEVDGVQIRRVRAWPAERDYYFAPGIYSQIRSGSWDVVHVQCYHTLV
                     TPLALLAARRANIPFLLTFHSGGHSSRARNAVRGIQRKILRPLLAPASRLVGVSQFEA
                     DFFHRHLQLPAAKFVIIPNGSQLPRVEEPSEPGEPGPLLVSVGRLEQYKGHQRIIRAL
                     PFILAEQPNARLRIAGTGPYESALRRLSEELGIADRVVIGPIPPVDRQGMARLLSQAA
                     LVTLLSDYEAHPVAVMEALALGRPVLVTDTSGLHEIATRWGVPSVPLASPAPAVAAAV
                     LEQLRRPWCPPTVELPTWDGCANALLTL"
     gene            complement(2131..>2926)
                     /locus_tag="VF276_17330"
     CDS             complement(2131..>2926)
                     /locus_tag="VF276_17330"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX6608665.1"
                     /translation="MYHSIGDKVSKRFAPFVVPPCQFAEQMAYLAEAGYTSLTVTQLV
                     AAWSGGLEALPARSVVLTFDDGFADFRTQALPVLERYGFRATLFVTTGFVGSTSQWLA
                     RDGAGERPLLSWRELSEVSAAGIECGGHSHTHPALDALPVARVRTEITECRDRLEQAL
                     GRVVCAFAYPFGYHSPSVQTAVREAGYTSACAVQYTASRPDNDRYALARLIVTRNTSL
                     KTFTQLVVSGGSTPDQLARRIRGAVWARIRHAHYGVIHTREAKGTA"
BASE COUNT          536 a          979 c          934 g          477 t
ORIGIN      
        1 agagggtgag caatgcattc gcacagccat cccaggttgg cagttccact gttggtgggc
       61 accagggccg gcgcaactgc tccagaaccg cagcagctac ggccggggcc gggctcgcca
      121 gcggtacgct cggaaccccc cagcgggtgg cgatctcgtg caagcccgac gtatcggtta
      181 ccagtaccgg gcgcccgagc gcgagcgcct ccatgaccgc caccgggtgt gcttcgtaat
      241 cgctcagcag ggttaccagc gccgcctgcg acagcaaccg ggccatgcct tgccgatcca
      301 cgggcgggat gggcccgatc accacccggt cggcaatgcc aagctcctca gagagccgcc
      361 gcaacgcgga ttcataaggg ccggtccccg caattcgcag ccgagcattc ggctgttcgg
      421 ccaggataaa gggcagcgcc cggatgattc gttgatgtcc cttatattgt tccaatcgtc
      481 cgacagaaac cagcagcgga ccgggctccc ccggttcgct cggctcctcg acgcgaggca
      541 actggctccc atttggtatt ataacgaatt tcgcggcagg taattgcagg tggcggtgaa
      601 agaaatcggc ctcgaactgc gacacaccaa ccagccgcga cgcgggagcg agcagggggc
      661 gaagtatctt gcgctggatg ccccgcaccg cgttgcgggc gcgcgaggaa tggccaccgc
      721 tgtgaaacgt gagcagaaac gggatgttgg cgcgccgggc ggccagcagc gccagcggag
      781 tcacgagggt atgatagcac tggacgtgca cgacatccca tgatccggag cgaatctgcg
      841 agtagatacc gggggcaaaa tagtagtcgc gctcggccgg ccaggcccgc actcgtcgga
      901 tctgcacccc gtcaacttcc tcctgcgccg gcagccggtc agtcgggtct gaggtcagca
      961 ccgtcacgtc cacgccggaa gccgccaggc ggcggctgac ctcgtaaacg tgcgtttcca
     1021 ccccgcccat ataggggaag tagcgggccg tgacctgcag cacccgcaga ggccgatccc
     1081 gcgtgggcgg gtgcacgttt gcgaccgggc ctcgcactgc cgttacagcg gccgggcctg
     1141 cggggaccgg cggtgcctcc agggtctgca ccgggatgtg ccgtcgtccc cggccggcag
     1201 gcgcttggcg ggccgtgcca agaatatagc ctgcgctcgt gcaggccaat ccgccaacaa
     1261 tagcagccgc ccgccccagc ccgcctggat cgcgccgcag tacgctatcg gccaggccat
     1321 gctgtacgcc gcgcggcagg gtgcgcaatg tgtaggcccg ctccgcggct agtccatcct
     1381 gggtgccgac caggcgggct atggccgcct tcgacaaacc ttccgagtag cagcgtctgg
     1441 caaagtagtc ccaacgtgcc cgctgcgccg ggatccggtg ccggatccgg gcgcgcggct
     1501 cgtatatgaa gatgccctgt ggatcgtgct gctgcaggcg aatgcaaaat tcggtttcct
     1561 caccgcctag gggccgggtt ccgattcgtc cgagcccgga gcggaagccg cccacagcgg
     1621 agaacgccgt ccggcggatg catccgcatc caccataaag gttgcggaca ggcgccgatt
     1681 cctgcggcag cccccggtac gtacagccga gcacccagaa gaattcatcg ggaaaccaac
     1741 gcggcgggtc gacctgccac agcggatcca cccgcccacc ggcacccagg acgtggggat
     1801 cggcacaagc gcccgtcaga tacgcgagcc agtcagggtc ggcttccgca tcgtcatcca
     1861 ggaaagcgac gagggcccct tgcgccacct cgatcccgct gttgcgggcg cctgaaaggc
     1921 ccctggcctc acgattctcg acaaccagca cgtcgggtag tgcggcgcgc gcgcgcgcca
     1981 atagctgggc attgtgatcg acgacaagga tgatttcccg cgccggcatc gattgcctgc
     2041 ggatcgattc gatcgcccgc tctatgtcaa accagcggtc ctccgtatac gcgcaaatta
     2101 ccaccgacac atcgggcagc atctccgtca tcaggcggtc ccctttgcct cgcgcgtgtg
     2161 tatgacgcca taatgcgcgt ggcggatccg cgcccagacg gcgccgcgga tgcgccgcgc
     2221 cagttgatcg ggggttgatc cgccacttac cactaactgt gtgaaggtct tcagacttgt
     2281 attcctcgtg acgatgagcc gggccagcgc atagcgatcg ttatcgggcc ggctggccgt
     2341 gtattgcacc gcgcatgccg atgtgtagcc cgcttctcgc accgcagttt gcactgaagg
     2401 actgtgataa ccgaagggat acgcgaacgc gcacacgacc cggcccaggg cttgctccag
     2461 gcggtcgcgg cattctgtga tttccgtccg gacacgcgca acgggcagcg catccagggc
     2521 cgggtgcgta tggctgtgcc ccccgcactc gattccggcc gcacttacct cgctgagttc
     2581 gcgccacgag agcaagggcc gttcgcccgc gccgtctcgc gcgagccact ggctggttga
     2641 tccgacaaag cctgtggtga cgaacagcgt cgccctgaaa ccatagcgct cgagaaccgg
     2701 tagggcctgg gtccggaaat cggcgaagcc atcgtcaaac gtcaagacga ccgatcgggc
     2761 cggtagggcc tctagtccgc cactccaggc cgcaaccagt tgcgtcacgg tgagcgatgt
     2821 gtagcctgct tcggccaggt atgccatctg ctcggcaaat tggcacggcg gcaccacaaa
     2881 cggagcaaag cgtttgctga ccttatcccc gatactgtgg tacatt
//
LOCUS       DASUYC010001062         3315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1766428, whole genome shotgun sequence.
ACCESSION   DASUYC010001062 DASUYC010000000
VERSION     DASUYC010001062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1766428"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            434..1534
                     /locus_tag="VF276_17335"
     CDS             434..1534
                     /locus_tag="VF276_17335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608666.1"
                     /translation="MHRFPRPVARGLAGLLVVTALLLGAVATPPAATRAADPPAPQHG
                     VAFVQLPTARFGSPASLESLRRLHATGANWVSIVAPFRSNGATYNTFYRSPNDPVDPE
                     LVAIIGAAHGMGMRVMLQPVVATDDGVWAGAIHPSTPAAWFDHYIEQIRNYARLAAGT
                     GVDELCIGTELFTMTGPQYSANWRAVIAAVKEHYSGPLTYSANWGDKKTPEYATIDWW
                     DALDAIGISAYFPLSWNNFYTEPLRQGWFTYTDPYGPNAHGQTFHWVDAITAVHNRWN
                     KPVQFTKIGFASYANSPGRWDLRPDPYLELSVQANAYDATMQAWQGAGWLTGMFWSPW
                     YSEPGAGGPLDTGESPQNKPAEQVLHHWYGGQ"
     gene            complement(1623..3194)
                     /locus_tag="VF276_17340"
     CDS             complement(1623..3194)
                     /locus_tag="VF276_17340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608667.1"
                     /translation="MTRLARIPAATWCVAGWIAAAALARAPLLTNQFHSIDEASYFAQ
                     AGRLHTLDAFVYAYLYRTETKSQVAVVPYLLAQLLAPAQAVLLVQVFALVAIVVSGAL
                     LIAAAGRWLGSPVIGLLAAGGWLPFLEVGPGYPLPGLDPTEAFPAPLLEYWQAPCVLA
                     AGYLALRAAESRGPRRAAPAWGLAGLAWALAVLIKPPALVLAPLLLAAPWALRVPASP
                     RTYLRNAVAFGVGALLPVAAVFGPYLPRPDTWAELRFNLVDVNAAYAEVVPLPLRAAA
                     MLLAFPPLLLLGWVAAPIVARRAAAGGPGRLLAGIWWAGPVFFAGLLPGGGFAHYIIP
                     LIPFVALATVGYGWWWLRGLAAAGRPRRAGTLAALLLGAYLLSQAPALLALPAQLSGD
                     SYLAGDRQRFDLDGLVAYIQAHSAPGDAIWVYYNTPELYTLAARLPATRMPNNEYLIA
                     YWREPWFSRTAADLAAEQPALLIGITDPRFARAWVTPLPAIPVVQDVIARLYRCDPAA
                     VRGAVICTRTPPAAR"
BASE COUNT          527 a         1167 c         1185 g          436 t
ORIGIN      
        1 taacaatacc tcaaggtcgt tgattatcaa gtaccctgat tatatcacgc accgtcctcg
       61 tgatcaagcg ggggcgggta gcctttagac gttcaccgcg aaggaacgaa ggcaacgaag
      121 agtgttgggg tttgtttcac cgcgggattt ttgcggattg gctatgtagt ggcccgctga
      181 ccgtattccc gcaaaccctc aactcccttc gcgcccttcg cgccttcgcg gtgcgatcaa
      241 accccgcaaa tcttcgttac cttcgttcct tcgcggtgaa taacgggcgc ctgaagcaag
      301 cgggtagcgg gggctggccc ggaatttgca ccggggaggc gaggggccgc ggcacacgcg
      361 ggcgcgccgg gcgcgggcgc gttccacacc ggcaatctgc acccgcccgg cccgcacgcg
      421 caggaggatc ttcatgcacc gtttcccccg cccggtggct cgtggcctgg ccgggctgct
      481 cgtcgtgacc gcgctgctgc tcggggccgt cgccaccccg cccgccgcca cgcgggccgc
      541 ggacccgccc gcgccccagc atggcgtggc ctttgtccag ttgcccaccg cccgcttcgg
      601 gtcgccggct tcgctggagt cgttgcgccg gctccatgcc accggtgcca actgggtgtc
      661 gattgtggcc ccgttccgca gcaacggcgc cacctataac accttctacc gctcgcccaa
      721 cgacccggtg gaccccgaac tggtggcgat catcggcgcc gcgcacggca tgggcatgcg
      781 cgtgatgctg caaccggtgg tggcgaccga cgacggcgtc tgggcggggg cgatccaccc
      841 cagcaccccg gcggcctggt tcgaccacta catcgagcag atccgcaact atgcccggct
      901 ggcggcgggc accggcgtgg acgaactctg catcggcacg gaactgttca cgatgaccgg
      961 gccgcaatat tcggccaact ggcgcgcggt gatcgccgcc gtcaaggagc actacagcgg
     1021 cccgctgacc tactcggcca actggggcga caagaaaacg cccgagtacg cgaccatcga
     1081 ctggtgggac gcgctcgacg ccatcggcat ctcggcctat ttcccgctgt cgtggaacaa
     1141 cttctacacc gagccgctgc gccagggctg gttcacctac accgacccct acggccccaa
     1201 cgcccacggc cagaccttcc actgggtgga cgcgatcacc gccgtgcaca accgctggaa
     1261 caagccggtc cagttcacca agatcggctt cgcatcgtat gccaactcgc cggggcgctg
     1321 ggacctgcgg cccgacccct acctggaact gagcgtgcag gccaacgcct acgacgcgac
     1381 catgcaggcc tggcagggcg ccggctggct cacgggcatg ttctggagtc cgtggtacag
     1441 cgagccgggc gcgggcggcc cgctggacac cggcgaaagc ccgcagaaca agcccgccga
     1501 gcaggtgctg caccactggt acggggggca atagcgagtg ctgagtacga gtggtcagtg
     1561 gtcagtggtc agtggtcagt aacgcattag gcttgtcact ctgagcgcag cgcagagtct
     1621 cgttagcgcg cggcgggcgg ggtgcgggtg cagatgacgg cgccgcggac ggcggcgggg
     1681 tcgcagcggt agaggcgggc gatcacgtct tgcacgaccg ggatggcggg caggggcgtg
     1741 acccaggcgc gggcgaagcg cgggtcggtg atgccgatga gcagggccgg ttgctcggcg
     1801 gccaggtcgg cggccgtgcg gctgaaccac ggctcgcgcc agtaggcgat gaggtactcg
     1861 ttgttgggca tgcgcgtggc cgggaggcgg gcggccagcg tgtagagttc cggggtgttg
     1921 tagtagaccc agatggcgtc gccgggcgcg ctgtgggcct ggatataggc cacgaggccg
     1981 tcgaggtcga accgctgccg gtcgccggcc aggtaggaat cgccgctcag ttgcgcgggc
     2041 agggcgagca gggcgggcgc ctggctcagc aggtaggcgc cgagtagcag cgcggcgagg
     2101 gtccctgcgc ggcgcggacg cccggcggcg gccaggccac gcagccacca ccacccgtag
     2161 cccaccgtgg cgagggccac gaacgggatc agcgggatga tgtagtgggc gaagccgccg
     2221 ccgggcagca ggcccgcgaa gaagaccggc ccggcccacc agatgcccgc cagcaggcgg
     2281 cccggcccgc ccgccgccgc ccgccgggcc acgatgggcg cggccaccca gccgagcagc
     2341 agcagcggcg gaaacgccag cagcatggcc gccgcccgca gcggcagcgg caccacctcg
     2401 gcataggcgg cgttcacgtc caccaggttg aagcgcagtt cggcccaggt gtcggggcgc
     2461 ggcaggtacg gcccgaacac ggcggccacc ggcagcagcg cccccacgcc gaacgcgacg
     2521 gcattccgca gatacgtccg cggcgacgcc ggcacgcgca gcgcccaggg cgcggccagc
     2581 agcagcggcg ccagcaccag cgcgggcggc ttgatcagca cggccagcgc ccaggccagc
     2641 cccgccagcc cccatgccgg cgcggcccgg cgtggccccc ggctctccgc ggcgcgcagg
     2701 gccaggtagc ccgcggccag cacgcacggc gcctgccagt attcgagcag cggcgcgggg
     2761 aacgcttcgg tcgggtcgag gccgggcagc ggatagcccg gccctacctc caggaacggc
     2821 agccagcccc ccgccgccag caggccgatg accgggctgc ccagccaccg ccccgccgcc
     2881 gcgatgagca gcgctccgct gacgacgatg gcgaccagcg cgaacacctg cacgagcagc
     2941 acggcctggg ccggggccag cagttgggcc agcaagtagg gcacgaccgc gacctggctc
     3001 ttggtctcgg tgcggtagag gtaggcgtag acgaaggcgt cgagcgtatg cagccgcccg
     3061 gcctgcgcga aatacgacgc ttcgtcgatg ctatggaact ggttggtcag cagcggcgcg
     3121 cgggccagcg cggcggcggc gatccacccg gcaacgcacc acgtggccgc cgggatgcgg
     3181 gcaagacggg tcaccacgat ctcccttggg ttgcgcgtgt attatagccg ggttgcggcg
     3241 cggggggcaa cctctccccc cggcccctcc ccgccgcggg gaggggagat cgctagcccc
     3301 ccttcccgtg gcggg
//
LOCUS       DASUYC010001063         2614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1106633, whole genome shotgun sequence.
ACCESSION   DASUYC010001063 DASUYC010000000
VERSION     DASUYC010001063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106633"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..649)
                     /locus_tag="VF276_17345"
     CDS             complement(158..649)
                     /locus_tag="VF276_17345"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX6608668.1"
                     /translation="MADKPLPTEQVLTMLAAAPPRIAAATAGRDPAQLQGRPAAEEWS
                     ANEVLAHLRACADMWGNCIMAMIAEDRPVLRAINPRTWIEKTDYLEQAFQPSLEAFTT
                     QRAALLAVLEPLPPEGWARAATVTGAGKALERTVHFYAQWLATHERPHLKQIESIVNT
                     LPG"
     gene            complement(917..1519)
                     /locus_tag="VF276_17350"
     CDS             complement(917..1519)
                     /locus_tag="VF276_17350"
                     /inference="COORDINATES: protein motif:HMM:NF025946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX6608669.1"
                     /translation="MAVTIDRFAQGMTFEAYVAQMSENRDKFMANYEKVQISAADRAA
                     ICGIGVPLNVLILTEDWCGDALTYVPVLGRLAACAGNWNLRIFKRDENLDLADQYLNF
                     GKFRSVPVVVFFDEQMRELGRFIERPASVNRERQQVIDGIAREHAEVQPGLRYNDQSD
                     AGKDLLAGPLRELRLSRQAAWEQAAIDEVCAALANVRAAA"
     gene            complement(1717..2073)
                     /gene="ssrA"
                     /locus_tag="VF276_17355"
     tmRNA           complement(1717..2073)
                     /gene="ssrA"
                     /locus_tag="VF276_17355"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2088..2558)
                     /gene="smpB"
                     /locus_tag="VF276_17360"
     CDS             complement(2088..2558)
                     /gene="smpB"
                     /locus_tag="VF276_17360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX6608670.1"
                     /translation="MSKREDGRRDLAVNRKAFHDYSIEETLEAGLVLVGSEIKSLRDG
                     KANLRESYVRIEGGEAWLINAHIGAYPQGGTHFNHEPLRRRKLLLHGNEIIYLRAKVE
                     QKGLTLVPLRIYLARGKAKLEIAVARGKKLYDKREALAERDARRDMERAVRGKA"
BASE COUNT          415 a          860 c          837 g          502 t
ORIGIN      
        1 acggccttcg gcgcggcggg ggccgggcac ctgcgcctct cctacggctc ggcccccgtg
       61 ccggtgctgg acgaggccgt ggcccgcctg cgcgccttct tcgcccgccg ctagtccacc
      121 gcgcgacata ccccgcaatt cggcgcagtg gcccgtgcta tccgggtagc gtattcacga
      181 tgctttcaat ctgcttcagg tgcggccgtt cgtgcgtcgc gagccactgc gcatagaagt
      241 gcaccgtccg ttctagggcc ttgcccgcgc ccgtcacggt tgccgctcgt gcccagcctt
      301 cgggcggcaa cggctccagg acggccaata gcgcggcccg ttgcgtcgta aaggcctcca
      361 gcgacggctg gaatgcctgt tcgagataat ccgttttttc gatccacgtc cgggggttga
      421 tggcgcgcag gaccggcctg tcttcggcga tcatcgccat gatgcagttg ccccacatgt
      481 cggcacacgc acgcagatgg gccagcacct cgttggccga ccactcctcg gcggcggggc
      541 ggccctgcaa ttgcgcgggg tcgcgccccg cggtcgccgc ggcgatgcgg ggcggcgcgg
      601 cggccagcat ggtcagcacc tgctcagtgg ggaggggttt gtcggccacg ggatgtctcc
      661 tttcgcagag gccagtggat cgataggcgg cggaccggtt tgaaaccggc ccctacaccg
      721 ccggttgggc gaaccggctt gaagccggcc cctacagcgc cagtcgcgcg ggccggttgg
      781 aaatccgccc gtacgcgatc atatcgggcg ggccggtttg aaagcggcgc ctgcaccggc
      841 caatcggcgg caaaaagatg ccgcctgcca ggggggtagg cggcattggt gcgggcttgg
      901 cgcggctgcg cgtgaatcag gcggcggcgc ggacgtttgc cagcgcggcg cagacttcgt
      961 cgatggcggc ctgttcccag gcggcctggc ggctcaggcg cagctcacgc aacggcccgg
     1021 ccagcaggtc tttgcccgcg tcgctctggt cgttgtagcg cagccccggc tggacctcgg
     1081 cgtgctcgcg ggcgatgccg tcgatcacct gctggcgctc gcggttgacg gacgccgggc
     1141 gctcgatgaa gcggcccagt tcgcgcatct gctcgtcgaa gaagacgacg accggcaccg
     1201 agcgaaactt gccgaagttg aggtactggt cggccaggtc gaggttctcg tcgcgcttga
     1261 agatgcgcag gttccagttg ccggcgcagg ccgccagccg ccccagcacg ggcacatagg
     1321 tgagcgcgtc gccgcaccag tcttcggtca ggatcagcac gttcagcggc acgccgatgc
     1381 cgcagatggc cgcccggtcg gcggcgctga tctggacctt ctcgtagttc gccataaact
     1441 tatcgcggtt ctcgctcatt tgcgccacat atgcctcaaa ggtcatacct tgcgcgaaac
     1501 gatctatcgt aacagccaca gtttccttcc tctcttgcac gtaatccggg acggcagcct
     1561 accgcatatt tccaagccgt aacctggtaa gccgttccac aatctattat agggaaagtt
     1621 tgcccttgcg cctggaatcc ggcttacact accggacaag gccggacaag caaaaagccg
     1681 gccgcgcctc atgcggggcg caaccggctt ttcggatggt ggagatgggg ggaatcgaac
     1741 ccccgtccag aaagctcagc cgataatatg ctacaagctt agtccgcgta tatttgagtc
     1801 actcaccgga cctccacgga cggagagccg gttcgccagc ccgatggtct tttgtcgcgg
     1861 ttatcaggcg gtccccgcgg cagcatcctg gttcatggcg cccggatccg ttctaccagg
     1921 aagagaacgg gcggacgtcg ccggagctag ttggtactag gcagcgagag caacaggctg
     1981 tttgccggtt gtggtttgct ctggattcac gaggtgagag cgcctcggct tgcaattacc
     2041 taaccttgcc ccctgtcgaa gccatgcatc cccaggtggc gcctgtctca ggctttgccc
     2101 cgcacggcac gttccatgtc gcgccgggca tcgcgctcgg ccagggcctc acgcttgtcg
     2161 tagagctttt tcccgcgggc gacggcgatc tccagcttgg ccttgccgcg cgccagatag
     2221 atgcgcagcg gcaccagggt caaccccttt tgctcgacct tcgcccgcaa gtagatgatc
     2281 tcgttcccgt gcaacagcag tttgcggcgc cgcagcggct cgtggttgaa gtgtgtgccc
     2341 ccctgggggt aggccccgat gtgggcattg atcaaccagg cttcgccgcc ttcgatgcgc
     2401 acatagctct cgcgcaggtt cgctttgcca tcccgcaagg acttgatctc ggatcccacc
     2461 agcaccaggc cggcttccag ggtttcttcg atactatagt cgtggaacgc cttgcggttg
     2521 accgcaagat cccggcgacc gtcctcacgc ttgctcacgc actaattgta ccagattcgg
     2581 cccgttttta caagccggtg gtcacgatgc ggta
//
LOCUS       DASUYC010001064         2366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1638417, whole genome shotgun sequence.
ACCESSION   DASUYC010001064 DASUYC010000000
VERSION     DASUYC010001064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1638417"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1776)
                     /locus_tag="VF276_17365"
     CDS             complement(<1..1776)
                     /locus_tag="VF276_17365"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEX6608671.1"
                     /translation="MRLHTRASVPARSGARIAGALLVLLALLLPVGLTPPAYAADQHQ
                     YWDNDEVTVQIHPDSTFDVTERQGFVFDSGTFHGSYRDIETNRLTGITNVSVSEEGVG
                     AYRPDNTKIDIENGDYGPPQTFQVLNESGHKRIIWFYGTLQSPAKKTMVIKYTVTGGL
                     RFYPGGDQFYWSPFMPDRNGIIEHATITVQVPPNIDLTTDPARKWAVYPADAGTVQVS
                     PHQAVFTANGVLGTSDDFEARLQWSHGLIQGSPAPWQEGDDRLRAFQAQWGPVFTLLA
                     LLGGAIILIGGSLFWLVRWYTKGRDPVTGPIADYLNEPPSDLPAGLVGVLLDEKADIR
                     DVIATILDLGRRGNLRIEEIKQKKGILGSESSDFQYTRLSMGGLQPYEQLVMNALFSG
                     SKNTRKLSDLKNQFYSKLPAINKAMYQALVDRKLFPRSPDETRNRNRALAGSLFLLAF
                     LSIFAAAFLGDLLTQALWIFPVAFAVPALFGFAMAGAMPQKTPKGAEQAARWQAFGRY
                     LADLQRFGDLGQAAQRFGEFLPYAVAMDLEKKYAQQFESVQDNMPMPTYYYPWGWNTG
                     YGSSTRDFSTGNGQRGGGSSSSGGGP"
     gene            complement(1886..>2366)
                     /locus_tag="VF276_17370"
     CDS             complement(1886..>2366)
                     /locus_tag="VF276_17370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608672.1"
                     /translation="MGVDQQYTRQFESVQAQMPMPTYYYPWGWMPGYGYGYGVPGVPG
                     ARGLAGAGVAGPGGLVESGGSRGIDFSPGGINAMNAGIVGSINSLNSGLTSMINDASS
                     VFVSTPSASSSGGWSGGGGFGGGGGGFGGFGGGGGGGSFGGGGGGGGGGGGGGGGSF"
BASE COUNT          344 a          804 c          810 g          408 t
ORIGIN      
        1 gggcccgccg ccgctgctgc tgcttccgcc gccgcgctgg ccgttgccgg tgctgaagtc
       61 gcgcgtgctg gagccgtagc cggtgttcca gccccacgga tagtagtagg tcggcatcgg
      121 catgttgtct tgcacgctct cgaactgctg ggcgtatttc ttttcgagat ccatggccac
      181 ggcatagggc aggaactcgc cgaagcgctg cgcggcttgg cccagatcgc cgaagcgttg
      241 caggtcggcc aggtagcgcc cgaaggcctg ccagcgcgcc gcttgctcgg cgcccttcgg
      301 cgtcttctgg ggcatggccc cggccatcgc gaagccgaac agggccggca cggcgaaggc
      361 caccgggaag atccacagcg cctgggtcag caggtcgccg aggaacgcgg cggcgaagat
      421 cgacaggaaa gcgaggagga agaggctgcc cgccagcgcc cggttgcggt tgcgcgtttc
      481 gtcggggctg cgcgggaaga gcttgcggtc caccagtgcc tggtacatcg ccttgttgat
      541 cgcgggcagc ttggaataga actggttctt gagatccgac agcttgcgcg tgttcttgct
      601 gccggagaac aacgcgttca tcaccaactg ctcgtagggc tgcagtccgc ccatgctcag
      661 ccgggtgtac tggaagtcgc tgctctcgct acccaggatg cccttctttt gtttgatttc
      721 ctcgatgcgc aggttgccgc gccggcccag gtcgagaatc gtggcgatca cgtcgcggat
      781 gtcggccttt tcgtcgagca gcacgccgac cagcccggcg ggcaggtccg acggcggctc
      841 gttgagatag tcggcgatgg gcccggtgac cgggtcgcgg cccttggtgt accagcggac
      901 cagccagaac aggctgccgc cgatcaggat gatcgcgccg ccgagcaggg ccagcagggt
      961 gaacaccggc ccccattgcg cctggaaggc gcgcagccgg tcgtcgccct cctgccaggg
     1021 cgcggggctg ccctggatca ggccgtgcga ccactgcaag cgggcctcga aatcgtcgct
     1081 cgtgccgagc acgccgttcg cggtgaagac cgcctggtgc gggctgacct gcaccgtgcc
     1141 cgcgtccgcc gggtagacag cccatttccg cgccgggtcg gtggtcaggt cgatgttcgg
     1201 cggcacctgc acggtgatcg tcgcgtgctc gatgatcccg ttgcggtcgg gcatgaaggg
     1261 gctccagtag aactggtcgc cgccggggta gaagcgcagg ccgccggtca cggtgtactt
     1321 gatgaccatc gtcttcttgg cgggcgattg cagcgtgccg tagaaccaga taatgcgctt
     1381 gtgcccgctc tcgttcagca cctggaaggt ctgcggcggg ccgtagtcgc cgttctcgat
     1441 gtcgatcttc gtgttgtcgg ggcggtaggc gccgacgcct tcctcgctga cgctgacgtt
     1501 ggtgatgccg gtcagccggt tggtctcgat gtcgcggtag ctgccgtgga aggtgccgct
     1561 gtcgaagaca aagccctgcc gctcggtcac gtcgaaagtc gagtccgggt ggatctggac
     1621 ggtgacttcg tcgttatccc agtactggtg ctggtccgcg gcataggccg gcggcgtgag
     1681 gcccaccggc agcaacaaag ccagcaggac cagcagcgcg ccggctatcc gcgccccgct
     1741 gcgcgcgggc accgatgccc tggtgtggag tcgcatttcc ctcttccatt ccgccccttg
     1801 cggggtctgt gaatcgatcg tatcgtttga gtagcgctag ccggcgcggc gatcagcgga
     1861 cccatctccg ccggggtggg cggcgctaga acgagccacc gccgcctcca ccgccgccac
     1921 cgccgcctcc gccgcctccg ccgaagctgc cgccgccgcc accgccgccg aagccgccga
     1981 agccgccgcc cccaccgccg aagccgcccc cgccgctcca accgccgcta ctcgacgcgc
     2041 tgggggtgct gacgaagacg ctgctggcgt cgttgatcat gctggtcagc ccgctgttga
     2101 ggctgttgat ggagccgacg atgccggcat tcatcgcgtt gatgccgccc ggcgaaaagt
     2161 cgatgccccg gctcccgccg gactccacaa ggccgcccgg cccggcgacg cccgcgccgg
     2221 ccagcccgcg ggcgcccggc acccccggca cgccgtagcc gtagccatag cccggcatcc
     2281 agccccaggg ataatagtaa gtaggcatcg gcatctgggc ctgcacgctc tcgaactggc
     2341 gggtgtactg ctggtccacg cccatg
//
LOCUS       DASUYC010001065         2332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_181699,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001065 DASUYC010000000
VERSION     DASUYC010001065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181699"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1685)
                     /locus_tag="VF276_17375"
     CDS             complement(<1..1685)
                     /locus_tag="VF276_17375"
                     /inference="COORDINATES: protein motif:HMM:NF016330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HEX6608673.1"
                     /translation="MPPTWTTEQILALAPDAGAAKAGKDLAAARKWLTLGHDAEVAWG
                     TCQGSGVAPYQTGIDLSGPGFRCSCPSRKFPCKHALSLFLLLANTPGSFTETARPEWI
                     AAWLAGRTRREAGAAKADGRTKAAANPAARARSAADRTARVAAGLQDLDLWMTDLVRH
                     GLATAQSRPAKFWETPAARLVDAQAPGVARLVRQLAGIPATGDGWPDRLLERLGRLHL
                     LGAGFRQIDSLPEPIQADIRGLIGWTQSQEELLAAPGLRDQWLVLGQRVTEEDRLRAQ
                     HTWLWGRTTGRAALILNYAHGNAPLDTSLLPGTAFEADLVYYAGAYPLRALIQARRDS
                     AALPATLPGYATIAEAIGAYAAALAAYPWLDELPLPLTGVVPVAHGDGWAVQDAEGRV
                     LPLHPQFNGGWMLLALSGGQPLGLFGEWNGEVLVPLSTWTGDRFYPLVSNEFTADGTS
                     GALAVTPPRALHEAHAALVSAALMGTERGSVSLPPLPGALGDLAAQLAGATPEHALLG
                     TAAALTLYERTGQVPARALAPLPEPAPAEDLPACSPAAGQHLAEMARGRHTILL"
     gene            2093..>2332
                     /locus_tag="VF276_17380"
     CDS             2093..>2332
                     /locus_tag="VF276_17380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HEX6608674.1"
                     /translation="MTRLLDSINGPADLKNRSIEELNQVAREVRELLIETIPAIGGHF
                     SSNLGTVELSVALHHVFDSPWDKIVWDVGHQGYPHK"
BASE COUNT          360 a          793 c          816 g          363 t
ORIGIN      
        1 agcaggatgg tgtgccggcc ccgcgccatc tcggccaggt gctgcccggc ggcgggcgaa
       61 caggcaggca aatcctccgc cggggccggc tcgggcagcg gggccagggc gcgggccggc
      121 acctggccgg tacgctcgta gagcgtgagc gccgccgccg tgcccagcag tgcgtgttcg
      181 ggcgtagcgc cggccagttg cgccgccagg tcgcccagcg cgccgggcag gggcggcagg
      241 ctcacgctgc cgcgctcggt gcccatcagc gccgcgctga ccagggccgc gtgggcctcg
      301 tgcagcgccc tgggcggggt cacagccagg gcgccggacg tcccatcggc ggtgaactcg
      361 ttgctgacca gcgggtagaa gcggtcgccg gtccatgtgc tcaagggcac cagcacttcc
      421 ccgttccatt cgccgaacag gcccaggggc tgtccgccgg acagcgccag cagcatccag
      481 ccgccgttga actgcggatg cagcggcagc acccggccct ccgcgtcctg cacggcccag
      541 ccgtcgccgt gggccacggg caccacgccg gtcagcggca gcggcaattc gtccagccag
      601 ggataagcgg ccagcgccgc ggcataggcg ccgatggcct cggcgatggt ggcgtagccg
      661 ggcagcgtcg cgggcagggc ggcggagtcg cggcgcgcct ggatcagcgc gcgcagcggg
      721 taggcgccgg cataatagac cagatcggcc tcgaaggccg tgcccggcaa gagactcgtg
      781 tcgagcggcg cgttgccatg cgcatagttc aggatcagcg cggcccggcc ggtggtccgg
      841 ccccagagcc acgtatgctg ggcgcgcagc cgatcctcct ccgtcacccg ctggcccagc
      901 accagccact ggtcgcggag accgggcgcc gccagcagtt cctcctggct ctgcgtccag
      961 ccgatgaggc cgcggatgtc cgcctggatc ggctccggca agctgtcgat ctgccggaag
     1021 cccgcgccga gcaggtgcag gcggcccagc cgttccagca ggcgatccgg ccagccgtcg
     1081 cccgtggcgg ggataccggc cagttggcgc accaggcggg ccacaccggg cgcctgcgcg
     1141 tcgaccagcc gggcggcggg cgtctcccag aacttggcgg gccggctctg cgcggtggcc
     1201 agcccatggc gcaccaggtc ggtcatccac aggtcgagat cctggaggcc cgcggccacc
     1261 cgcgccgtgc ggtcggcggc ggaccgggcg cgcgccgccg ggttggcggc ggccttcgtg
     1321 cgcccgtcgg ctttggccgc tccggcctcg cggcgcgtcc gccccgccag ccacgcggcg
     1381 atccattcgg gccgcgccgt ctcggtgaag ctacccggcg tgttcgccag cagcaggaac
     1441 aggctgagcg cgtgcttgca ggggaacttg cggctggggc aactgcagcg aaaccccggc
     1501 ccgctcaggt cgatccccgt ctgatagggc gctacgccgc tgccctggca ggtgccccag
     1561 gccacctcgg cgtcatggcc cagcgtgagc cacttgcgag cagcggccag atccttcccg
     1621 gctttcgcgg ctccggcatc gggggcaagc gcaaggattt gctcggtcgt ccaggttggg
     1681 ggcatccatg cgtctccgtc tcatccagac tggtgggtct cggtggaacc gtccggccac
     1741 cggtccggtc cacgaccctg tttcctatta tatccataat cggcccggag atccggaatc
     1801 cgcccattcg cgccgcaccg tgattttgcg ctattgactg aattttcaga aagtaatata
     1861 atgttgtgaa agcggggagt cccggctccc ggcatctgac aggcggatgt aagcgttcac
     1921 tacctggatc gttataaata tggcgctgcg gtgtatcgaa cgatgcacgc tacagaattg
     1981 acacaggggc gctaactctg gtaatattgc tggcaatgtc cgcaaacttg agggcgttcc
     2041 gggacgacac gtcaggcaag gggcctggaa ttatgaagga atggatggtg ccatgactcg
     2101 ccttttggac tctatcaacg ggccggccga cttgaaaaac cgatctatcg aagagctgaa
     2161 tcaggtggcg cgcgaagtgc gcgaactgct gatcgagacg attcccgcca ttggcggcca
     2221 cttttcgtcg aacctcggca cagtcgagtt gtccgtcgcg ttacatcacg ttttcgatag
     2281 cccgtgggat aagatcgtgt gggatgtcgg ccaccagggc tacccgcaca ag
//
LOCUS       DASUYC010001066         5098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1335627, whole genome shotgun sequence.
ACCESSION   DASUYC010001066 DASUYC010000000
VERSION     DASUYC010001066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5098
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1335627"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1248)
                     /locus_tag="VF276_17385"
     CDS             complement(<1..1248)
                     /locus_tag="VF276_17385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019492046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HEX6608675.1"
                     /translation="MADTQSILTTLRGAAQPFTGAPADYTPLLDLIGDARFVLIGEAS
                     HGTRDFYHERAEITKRLIRDKGFTAVAVEADWPDAYQINRYVRGSGDGATAIDSLAGF
                     ERFPTWMWRNTVVLDFIGWLRDYNDNRPTGAAKAGFYGLDLYSMYGSIRAVLEYLDKV
                     DPEAAQRARYRYSCLEHFGEDSQAYGYAASFGMSQPCEDEVIKQLVELQHHGADYSSR
                     DGRVAEDEFFFAEQNARLVKNAEEYYRSMFHGRVSSWNLRDTHMVETLDALVGHLDRQ
                     GGRSKVVLWAHNSHLGDARATQMGQGGEVNIGQLVRERHDGDTALIGFTTYTGTVTAA
                     SDWDEPAERKRVRPGLPGSYEALFHEVGIPRFWLPLHSGPAADALRRPRLERAIGVIY
                     RPDTERISHYFTAELPAQFDAVIH"
     gene            complement(1267..1968)
                     /locus_tag="VF276_17390"
     CDS             complement(1267..1968)
                     /locus_tag="VF276_17390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015183423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEX6608676.1"
                     /translation="MTTGVFHDRRDAGRQLATRLATYAGRPDVLVLALPRGGVPVGFA
                     VAQALQVPLDVFLVRKLGVPGHEELAMGAIATGDVVVLNEDVVRELGIPERVLAAVVA
                     QERQELARRDHLYRDGRPMPAIQDRVIILVDDGLATGATMRAAAQAIRAQQPARLVIA
                     VPVGATPTCEALQAEADEVICLLTPTPFYAVGLWYEDFSPTSDQEVRADLAEAAARSL
                     AAPGGPAPPAAGTYA"
     gene            complement(2022..2495)
                     /locus_tag="VF276_17395"
     CDS             complement(2022..2495)
                     /locus_tag="VF276_17395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEX6608677.1"
                     /translation="MTFRLTSSAIADGQPIPRRYSCEEHSISPPLTWDDVPAGTQSFA
                     LLLDDPDAPHGVYTHWVLYGIAAGARAIPENVIPGKEGPDGSRQGRNSAGQPGYTGPC
                     PPRGTHRYVFDLYALDTTLDLPPGATKRELIDAMGTHIIAEAQLTGTYQKHTPGG"
     gene            complement(2989..4005)
                     /locus_tag="VF276_17400"
     CDS             complement(2989..4005)
                     /locus_tag="VF276_17400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608678.1"
                     /translation="MKTLMISRTASIRSYVMVCLTVLLTITALVPQAGYAMPADPDPT
                     RAAPAVVRAWQPGPYYPQIGPSCTGYALAAWLGSEPQPIATHPLGWQIYDMADARDGL
                     VGPHHGTTVAAGVAVLTDLGYVRASRTTSNPDSALAFLRTSGPLVIEGPFPGGIPSLD
                     SAGNLRWAGRLYYHAWICYGLEAGDRLACQDSLATAWNARVGGRFHMSRASLVTVLAG
                     GRAWLLQKAPTPPAAEQQSRWAYSPEDYIALVYGGQAATTPDWGTREAELHQLFDAGH
                     WDVDDTVGSRSNRYRLTWDSWRAEHITYIAEPNAYARKIAVEQWKGTLPGPGVPHVLL
                     VAPL"
     gene            complement(4257..4442)
                     /locus_tag="VF276_17405"
     CDS             complement(4257..4442)
                     /locus_tag="VF276_17405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608679.1"
                     /translation="MYQDYINQARAAQHRQDLLTEAANERSLRAVRQMRQEAQQSEPP
                     RTPKPQTPRRLGLHPAR"
     gene            complement(4785..4970)
                     /locus_tag="VF276_17410"
     CDS             complement(4785..4970)
                     /locus_tag="VF276_17410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608680.1"
                     /translation="MYQDYISQARAAQHRQDLLTEAANERSLRAARQMRQEAQQSDQP
                     RLHRPQGRRQLSLRPAR"
BASE COUNT          750 a         1613 c         1806 g          929 t
ORIGIN      
        1 atggatcacg gcgtcgaatt gcgccggcaa ctcggcggtg aagtagtggc tgatgcgctc
       61 ggtgtccggg cggtagatga cgccgatggc gcgttccagg cgcgggcggc gcagcgcgtc
      121 cgcggcgggg ccgctgtgca ggggcagcca gaagcgcggg atgccaacct cgtggaacag
      181 ggcctcgtag ctgcccggca gccctgggcg cacccgtttg cgttccgccg gttcgtccca
      241 gtcggacgcc gccgtcacgg tgccggtgta ggtggtgaag ccgatcagtg cggtgtcgcc
      301 atcatggcgc tcgcgcacca gttggccgat gttcacctcg ccgccctggc ccatttgcgt
      361 ggcgcgggcg tcgcccaggt gcgagttgtg cgcccagagc acgaccttgc tgcgcccgcc
      421 ttgccggtcg agatggccca cgagtgcgtc gagcgtctcg accatatgcg tgtcgcgcag
      481 gttccaggac gagacgcgcc cgtggaacat gctgcggtag tattcctcgg cgttcttcac
      541 caggcgggcg ttctgttcgg caaagaagaa ctcgtcttcc gccacgcggc cgtcgcggct
      601 gctgtagtcg gcgccgtggt gctgcaactc cacaagctgt ttgatcacct cgtcctcgca
      661 gggctggctc atgccgaagc tggcggcgta accgtaagcc tgcgagtctt cgccgaagtg
      721 ttccaggcag ctatagcggt agcgggcgcg ctgggcggcc tccgggtcca ccttgtcgag
      781 atactccagc acggcgcgga tcgagccgta catgctatag aggtccagcc cgtagaaccc
      841 ggccttggcc gcgcccgtgg ggcggttgtc attgtagtcg cgcaaccagc cgatgaagtc
      901 gagcaccacg gtgttgcgcc acatccaggt tgggaagcgc tcgaagcccg ccagcgagtc
      961 gatggccgtg gccccgtcgc cgctgccgcg cacgtagcgg ttgatctgat acgcgtcggg
     1021 ccagtcggct tcgacggcga cggcggtgaa gcccttgtcg cggatcagcc gcttggtgat
     1081 ctcggcgcgt tcgtggtaga agtcacgggt gccgtgcgag gcttcgccga tcagcacgaa
     1141 ccgtgcatcg ccaatcaaat cgagcagcgg ggtgtaatcg gcgggcgcgc cggtgaaggg
     1201 ctgggcggcg ccgcgcaggg tagtaaggat gctctgggtg tcggccatgg ttggcagcct
     1261 cctgttctat gcgtaggtgc ctgcggcggg cggcgccggg ccgccgggtg cggcgaggga
     1321 gcgcgcggcg gcctcggcca ggtcggcccg cacctcctga tcgctggtgg gcgagaaatc
     1381 ctcgtaccag aggccgacgg cgtaaaacgg cgtgggcgtc agcaggcaga tcacctcgtc
     1441 ggcctcggcc tgcaacgcct cgcaggtcgg ggtggcgccc accggcacgg cgatcaccag
     1501 ccgcgcgggc tgttgggcgc ggatcgcctg ggccgccgca cgcatggtcg cgcccgtcgc
     1561 caacccgtcg tcaaccagga tgatgacgcg gtcctggatc gccggcatgg ggcggccgtc
     1621 gcggtagaga tggtcgcggc gggcgagttc ctggcgctcc tgggccacca cggcggcgag
     1681 cacgcgttcc gggatgccca gttcgcgcac cacatcttcg ttcaacacga ccacgtcgcc
     1741 ggtggcgata gcgcccatcg ccagttcttc gtgccccggc acgccgagtt tgcgcacgag
     1801 gaacacgtcg aggggcacct gcagcgcctg ggccacggcg aaccccaccg gcacgccgcc
     1861 gcggggtagg gccaggacca ggacatcggg gcgcccggcg taggtcgcca gccgggtggc
     1921 cagttgccgg cccgcatccc tgcggtcatg aaatactccc gttgtcatgt gtgttccccc
     1981 tcgctgtgcg cacagttacc gccggcgcgc ggcgcgagcg actagccgcc cggtgtgtgc
     2041 ttttgatacg taccggtcaa ttgcgcctcg gcgatgatat gggtgcccat cgcgtcgatc
     2101 agctcgcgtt tggtcgcgcc cggcggcagg tccagcgtcg tgtccagcgc gtagaggtcg
     2161 aagacgtagc ggtgcgtgcc gcgcggcggg cacgggccgg tatagcccgg ttggcccgcg
     2221 ctgttgcggc cctggcggct gccgtcgggg ccctccttgc cggggatcac gttctccggg
     2281 atcgcccgcg ccccggcggc gatgccatac agtacccagt gcgtgtagac accgtgcggc
     2341 gcgtcggggt cgtcaagcag cagggcgaaa ctctgcgtgc ccgccggtac gtcgtcccag
     2401 gtcagaggcg gggagatgct gtgttcttcg cagctatagc gccgcgggat gggttgcccg
     2461 tcggcaatcg cggaactggt gagtcgaaag gtcatgctgg tgaatcctcc tcgttgaggt
     2521 tgcgttgtgc cggtccggtc ggcgggccgg tcggatttgt gccttttaat tcgtagcaaa
     2581 gtatgtgcca gaaatcagcg ctgtattgtg ttgttatact acaagtgtct gtcatgggtg
     2641 cagtgaagat cgctgtattt gttgttgaga acgcttttgt cgcgcagagg agcttgccgg
     2701 gttcatacgc gagtcttcaa tcgttgttta tcccacaccc tgaaagaatt atagatctat
     2761 ttcgcgccgg cacaggggtg atcgacagag gtacgcggtg cgcggagtcc gcgatctggt
     2821 taagggagat cgtagactcc accttataca ggatacatcg gttgccttaa caccgcagtg
     2881 aaccggggtg tacagttggt aacaagggcg ccgggaaccg agaccgcctt cgctcccgcg
     2941 ggagcgaagg cggtatcacc cggcaaggat tgcgggctgc gactggtatt acagcggggc
     3001 gaccagcaac acgtgcggca cccccggtcc cggcagcgtg cccttccact gttcgaccgc
     3061 gatcttgcgg gcataggcgt tcggttccgc gatgtaggtg atgtgctcgg cgcgccagct
     3121 atcccaggtg aggcggtagc ggttagagcg cgagccgacc gtgtcgtcta cgtcccaatg
     3181 gccggcgtcg aacaactggt gcaactcggc ttcgcgcgtg ccccaatccg gcgtggtggc
     3241 agcctggccg ccataaacca gcgcgatgta gtcctcaggg ctgtaggccc agcggctctg
     3301 ttgctcggcg gcgggcgggg tcggggcctt ctgcagtaac caggcccggc cgccggccag
     3361 caccgtgacg aggctggcgc gcgacatatg gaagcggcca cctacgcggg cgttccacgc
     3421 cgtggcgagg ctgtcctggc aggccagccg gtcgccggcc tccaagccgt agcagatcca
     3481 ggcgtggtag tacagccggc cggcccaccg caggttcccc gcgctgtcca ggctagggat
     3541 tccgccgggg aaggggcctt cgatcaccag cgggccgctg gtgcgcagga aggccagggc
     3601 gctgtccggg ttggaggtgg tccgcgaggc gcgcacgtaa ccgaggtcag tcaggacggc
     3661 cacgcccgcc gcgacggtgg tgccgtggtg cggacccaca aggccgtccc gcgcgtcggc
     3721 catatcgtag atctgccagc ccagcgggtg cgtggcgatc ggctgcggtt cgctgccaag
     3781 ccaggcggcg agcgcatagc cggtgcacga ggggccgatt tgcgggtagt acgggccggg
     3841 ctgccacgcg cggaccaccg ccggcgcagc gcgtgtcggg tccgggtctg ccggcatggc
     3901 gtaaccggcc tggggtacga gggcggtgat ggtgagcagg accgtcaggc agaccataac
     3961 gtacgagcga atagacgctg tccggctgat cattaaggtc ttcatctgga gcctcctgtg
     4021 cggatcggcg ctgtatcttc cgatcgactc cagcgtagga ccaggatgcc aaagaactgc
     4081 caacactctc gttggcatag cgaaggggtc cagcatatcg tcgctggacc ccttcgccat
     4141 tgtgcgtctt cacgttgccg gtgggcggcc cgatgacgcg ccgtcacggc gccactccgt
     4201 ggatcgcaaa cgcccgtcct ctcggatggg cgtctgcgcc gccgaaccct gggcggctat
     4261 cgggccggat gcaagcctaa ccggcgcggg gtttgcggct tgggcgttcg cggtggttcg
     4321 gattgttgcg cctcctggcg catctgccgg acggcgcgca ggctgcgctc gttggccgct
     4381 tcggtcagca gatcctgccg gtgctgggcg gcgcgcgcct ggttgatata gtcctgatac
     4441 attgtggtcc tccttttcca tgcggcgcgc tcgggcccaa tcccttgccg cggcgcccgc
     4501 acgcgcggga tacacttgtc gatctgccgt catcttcgga tcggcgctct gtcttccgat
     4561 cgactccaat gtaggaccgg ggtgccaaag aactgccaaa actctcgtcg gcatgaaaaa
     4621 ggggtccagc atatctttgc tggacccctg caatgtcgtg tggctctacg gccccgtcgg
     4681 gagcggtaat gttcgtccgc cacggaatgc gggcggatcg cggccgggga tgacagacgc
     4741 ccgtccgaga gggacgggcg tctgccccgt tcaacaccgg gcggctaccg agccggacgc
     4801 aagctcaatt ggcgccggcc ctgcggcctg tggagccgcg gttggtcgga ttgctgcgct
     4861 tcctggcgca tctgccgcgc ggcacgcagg ctgcgctcat tggccgcctc ggtcaggagg
     4921 tcctgccggt gctgagcggc gcgcgcctgg ctgatataat cctgatacat cgtggtcctc
     4981 ctttccggag cggcgcgctg gggccgggtc acttgccgtt ggcccgcacg cgcgggatac
     5041 cagtgtcgtt ctgccgtcat ctatcatcat ctggtgatgg ttgccggctt ctgattca
//
LOCUS       DASUYC010001067         5061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_2048813, whole genome shotgun sequence.
ACCESSION   DASUYC010001067 DASUYC010000000
VERSION     DASUYC010001067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2048813"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1479
                     /locus_tag="VF276_17415"
     CDS             <1..1479
                     /locus_tag="VF276_17415"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6608681.1"
                     /translation="TIDAYVKSYKKIDNYTVQIETTHPAYDFVTTLGVYTWGSAFNIV
                     PKHIFEKQTDLAAFKNTNPVTLGAYTIKAFDPNGQWHLWQRRDDWQRSSTGSLGTPTP
                     KYVLYKNFGDEQTRTLAFIKNEYDVDTFMSPDSIAAAQKQNPNVQTFAKTLPYHDMGD
                     ACSYGIIMNQQKAPLDNANVRWALALSLDLANVGTNAMSGQFRASALPMPDTQITRPA
                     FFDPLQQWLKDLKLSDGYQPYNPNFGADMVKKLTDMGMDAATLPQGEAVTQGFGLGWW
                     KADPAEAEKLMNSAGYKKGADGFYAGPDGKTWQVELVIPSDWNKVMQRVGFSIADAWT
                     KAGFKVNARQVDNGEFTKVQNTNALLTTMVNWSTSCVFNTNYLNAFRSINKDNVLPAD
                     SNDALSGNQDRITDQKVFDLVTKANSLDQSAPEFLQTGQDIIKEIVQNMYYINMMNIP
                     TTIPTNSTYWTGFPKADNTYAVPYTWWSSFKKILVNIKPTGK"
     gene            1597..2601
                     /locus_tag="VF276_17420"
     CDS             1597..2601
                     /locus_tag="VF276_17420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6608682.1"
                     /translation="MKLAQYVLQRFVSYLLVLFIGITITFFLPRLMPGDPIENYISQV
                     QSRAGQTMTPEAVAQLRDSLAELYGLKGDLFSQYLSFLNRVFLHFDFGPSFTSYPEPV
                     SNILMAALPWTAGLLVTTTILSWLLGNLIGLVAGYFNKHPAAAALEIFGIVLYPIPYY
                     ILALTVIIVLAFFFPIFPIAPTFTPGALTLRKIGVILYNSFLPALTLVLAGFGWNILS
                     MKSLAVATKEEGYVTFARLKGTPDWTRMTRYVFRNALLPQVTALVLSLGTIFNGALLT
                     EMLFNYPGVGLLMRTAAGAGDYNLLYGAITLAIISVATAALVLDLCYPLFDPRIRLGR
                     "
     gene            2607..3506
                     /locus_tag="VF276_17425"
     CDS             2607..3506
                     /locus_tag="VF276_17425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007257168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6608683.1"
                     /translation="MKETVSVSSSVAAKPAVATPVQRRLPKLNGRLYIGLTITMILVL
                     GSLILPLFTDVDPAAQGTYLKNLPLSGKHLLGTNALGQDIFWFLVFAMRNSLLLGVGV
                     SLGITAISTFLGISAGYIGGWYERIVVIFIDTFITIPVLPILIVLGALVRGNTSFAIV
                     GVILICFGWAWNARTIRSMALSLREREFINMARFSGARTFDIISREIFPYVYAYMVVG
                     FINTILFAINTEAALAVIGLSKVQIPTLGSIIFWALNYNALMIGQTAWIIAPIVATVL
                     LFLGLFLTSTGYNELFAERRGVA"
     gene            3503..4597
                     /locus_tag="VF276_17430"
     CDS             3503..4597
                     /locus_tag="VF276_17430"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6608684.1"
                     /translation="MIKLDNIVATYTTQRGQVSAVDGVTLEIPDSLILGIAGESGCGK
                     STLMKVIYGDIGFPLGLASGSVDYGFQNERKQPVTTKNIQREWFKRISYIPQSSMSSL
                     NPVVRIREQFVDFPATSKQKAQTLERVREFVQKLGLPPESIDSYPHQLSGGMRQRIMV
                     AMATFLQPDIILADEPTTALDVVVQKSILILLMDLQKEMKNTIIFVSHDMGVHYQLTD
                     KMLIMYAAKAVEYANTPDIFREPLHPYTLMLTSSLPTIGDDRARQGLPGRPPSLWDPP
                     AGCRFAPRCPLATELCRTVEPPLAEHRPGRLSACHYAESVPQLKQTLQNAPKLSEVTN
                     EPTKLIAQVQRELKLDETIDQEVKVEEVAR"
     gene            4594..>5061
                     /locus_tag="VF276_17435"
     CDS             4594..>5061
                     /locus_tag="VF276_17435"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6608685.1"
                     /translation="MKTILQTTNLNKEYQLGSFPRNVTIRALNNINLTVESDRPVIIS
                     IVGESGSGKTTLAKVILRLVPPTSGNVTIGGHVVAGKGAHPDKAAFLRTVQPIFQNPF
                     EAFSRYRTIDSYLYETATRVAGLSKDAAVQALEEALAVVGLEYKDVRGKYPNQF"
BASE COUNT         1024 a         1627 c         1465 g          945 t
ORIGIN      
        1 accatcgacg cctacgtcaa atcctacaag aagatcgaca actacacggt ccagattgag
       61 accacccacc cggcctacga cttcgtcacc acgctgggcg tctatacctg gggctcggcg
      121 ttcaacatcg tcccgaagca catcttcgag aagcaaaccg accttgccgc tttcaagaac
      181 accaacccgg tcacgctggg cgcgtacacg atcaaagcct tcgatcccaa cggccagtgg
      241 cacctctggc agcggcgcga cgactggcag cgctcgtcca ccggcagcct gggcacgccg
      301 acgcccaagt atgtcttgta taagaacttc ggcgacgagc agacccggac cctggccttc
      361 atcaagaacg agtacgacgt cgatactttt atgagcccgg acagcatcgc cgcggcgcag
      421 aagcagaacc ccaacgtcca gacgtttgcc aaaaccctgc cttaccatga catgggcgat
      481 gcctgctcgt atggcatcat catgaaccag cagaaggcgc ccctggacaa cgccaacgtg
      541 cgctgggcac tggccctgtc gctcgacctg gcgaacgtcg gcaccaacgc catgagcggc
      601 cagttccggg cgtcggccct gcccatgccc gacacgcaga ttacacgccc ggccttcttc
      661 gacccgctgc agcagtggct caaggatttg aagctgtcgg acggctatca gccctacaac
      721 ccgaacttcg gcgcggacat ggtcaagaag ttgactgata tgggcatgga cgccgccacc
      781 ctaccccagg gcgaagcagt cacccagggc ttcggtctcg gctggtggaa ggctgacccg
      841 gctgaggcgg agaagttgat gaactcggcc ggctacaaga agggcgcaga cggcttctac
      901 gccgggcccg acggcaagac ctggcaggta gaactggtca tccccagcga ctggaacaag
      961 gtcatgcagc gcgtcggctt ctcaatcgcc gatgcctgga ccaaggccgg cttcaaggtg
     1021 aacgcccgcc aggtggacaa cggcgaattc accaaggtgc agaatacgaa tgccctgctc
     1081 accaccatgg tgaactggtc tacgagctgc gtcttcaaca cgaactatct caacgccttc
     1141 cgttcgatca acaaagacaa cgtcctgcct gcggactcga acgacgcgct gagcggcaac
     1201 caggaccgca tcaccgacca gaaggtgttc gacctggtta caaaggcgaa ttcgctggac
     1261 cagtcggcgc ccgaattcct gcagaccggc caggacatca tcaaagagat cgtccagaat
     1321 atgtactaca tcaacatgat gaacatcccg acgacgatcc cgacgaacag cacgtactgg
     1381 acgggcttcc cgaaggccga caacacctat gccgtcccgt acacctggtg gagttcgttc
     1441 aagaagatcc tggtcaacat caagcccacg ggcaagtaat acaccgcaaa cacggtgggg
     1501 gccgcagccg ccgcggcccc cacccaatca gggacaacag gttgattggc actcgggtca
     1561 acgcttgcgg cgacccacgc gtggatgagg tgcatcatga aactagctca atacgtcctg
     1621 cagcggttcg tgagctatct gctggtcttg ttcatcggga tcaccatcac gttcttcctg
     1681 ccccggctca tgcctgggga cccgatcgag aactatatct cgcaggtcca gtcccgcgcg
     1741 gggcagacga tgaccccgga ggccgtcgcg cagttacgcg actcgcttgc tgagctctac
     1801 ggcctgaagg gcgatctctt cagccagtat ctcagtttcc tgaaccgcgt gttcctgcac
     1861 ttcgacttcg gtccgtcgtt cacctcctac ccggagccgg tgtccaacat cttgatggcc
     1921 gcgctgccct ggaccgcggg actgctcgtg accaccacga tcctctcctg gctactgggc
     1981 aacctgatcg ggctggtggc cggctacttc aacaaacacc cggccgcggc agcgctggag
     2041 atcttcggca tcgtgctgta tccgatccct tactacattc tggcgctaac ggtcattatc
     2101 gtgctcgcct ttttcttccc gatcttcccg atcgcgccca ccttcacgcc cggcgccctg
     2161 acattgcgga aaatcggggt catcctgtac aactctttcc tgcccgccct caccctcgtg
     2221 ctggccgggt tcggctggaa tatcctgagt atgaagtcgc tggcggttgc caccaaagag
     2281 gagggctacg tcaccttcgc ccggctcaag ggcacgcccg actggacgcg gatgacccgc
     2341 tatgtcttcc gtaacgccct gttaccgcag gtgacggcgc tggtgctctc gctcggcacg
     2401 atcttcaacg gggcgctgct cacggagatg ctattcaact atccgggcgt cgggctgttg
     2461 atgcgcaccg ccgccggtgc cggcgattat aacctgctct acggcgcgat taccctggcg
     2521 atcatttcgg tggcaacggc ggcgctggtc ctcgatctgt gttacccgct gttcgacccg
     2581 cgtatcaggc tagggaggtg accaacatga aggaaaccgt gagcgtcagc agtagcgttg
     2641 ccgccaagcc cgccgtcgcc accccggttc agcgccggct cccgaagctg aacggacggc
     2701 tgtacatcgg gttgacgatt accatgatcc tggtcctcgg gagcctcatc ctgccgctgt
     2761 tcacggacgt tgatccggcg gcgcaaggca cctacctgaa gaacctgccg ctgagcggca
     2821 agcatctgct cgggaccaat gcgctcgggc aagacatctt ctggttcctc gtctttgcga
     2881 tgcggaattc gctgctgctg ggcgtcggtg tgagccttgg tatcaccgcg atctccacct
     2941 tcctgggcat aagtgccgga tacatcggcg gctggtacga gcggattgtc gtgatcttca
     3001 tcgacacgtt catcaccatc ccggtgctgc ccatcctgat tgtcctggga gcgctggtgc
     3061 gcggcaatac ctcgttcgcc atcgtcggcg tgatcctgat ctgcttcggt tgggcgtgga
     3121 acgcgcggac gatccgctcg atggccctgt cgctccggga acgtgagttc ataaacatgg
     3181 cgcgcttctc cggcgcgcgc accttcgata tcatctcccg cgagatcttt ccctacgtgt
     3241 acgcctatat ggtggtcggc ttcatcaaca cgatcctgtt cgccatcaat acggaggcgg
     3301 ccctggcggt gatcggcctc tcgaaagtcc agatacccac gctggggtcg atcatcttct
     3361 gggcgctgaa ctacaacgcc ctgatgatcg gccagaccgc ctggatcatc gcgcccatcg
     3421 tggcgacggt gttgttgttc ctcggcctgt tcctgacctc gacaggctac aatgagctct
     3481 tcgcagaaag gcggggtgtg gcatgatcaa gctggacaat attgtggcga cctacaccac
     3541 gcaacggggc caggtcagcg ccgtcgacgg cgtgaccctg gagatccccg acagcctgat
     3601 cctgggcatc gccggcgagt cagggtgcgg caaaagtacg ctgatgaagg tcatctacgg
     3661 cgacatcggc ttcccgctgg ggctggcgtc cgggtcggtc gattatggct ttcaaaacga
     3721 gcgcaagcag ccggtgacga ccaagaacat tcagcgggaa tggttcaagc gcatctccta
     3781 catcccacag agttccatga gttcattgaa cccggtcgtg cgcatccgcg agcagttcgt
     3841 cgatttcccg gccacctcga agcagaaggc gcagacgctg gagcgggtgc gggaattcgt
     3901 gcagaaactg gggctgccgc ctgaatcgat cgactcctat ccgcaccagc tttccggcgg
     3961 catgcgccag cgcatcatgg tcgccatggc gaccttcctg cagccggaca tcatcctcgc
     4021 cgatgagccc acgacggcgc tggatgtcgt ggtgcagaag agcatcctga tcttgctgat
     4081 ggacctgcag aaggagatga agaacaccat catcttcgtc tcccacgaca tgggcgtcca
     4141 ctaccagctc acggataaga tgctgattat gtacgccgcc aaagcggtcg agtacgccaa
     4201 caccccggac atcttccggg agccattgca cccctatacc ctgatgctga ccagttcgtt
     4261 gccgaccatt ggcgacgatc gcgcgcggca aggcctaccg gggcgcccac cgagcctctg
     4321 ggacccgccc gcggggtgcc ggttcgcccc gcgctgcccc ctggcaacgg aactgtgccg
     4381 aaccgtcgag ccgccgctgg cggagcatcg ccccggacgc ctctccgcct gccactatgc
     4441 cgagagcgtg ccgcagttga aacagacgtt gcagaacgcg ccgaagctga gcgaggtgac
     4501 caacgagccg acgaagttga tcgcgcaagt ccagcgcgag ttgaagctgg acgagacgat
     4561 cgatcaggaa gtcaaggtgg aagaggtggc ccgatgaaga cgattctaca gaccacgaac
     4621 ttgaataaag agtaccagtt gggcagcttt ccgcgcaacg tgaccattag ggcgctgaac
     4681 aacatcaacc tgacggtgga gagcgaccgg ccggtgatta tcagcatcgt cggggagtcg
     4741 ggaagcggca aaaccacgct agccaaggtc atcctgcggc tggtaccgcc cacgtcgggc
     4801 aacgtgacca ttggcggcca tgttgtcgcc ggcaaggggg cccacccgga taaggccgcg
     4861 ttcttacgga ccgtgcagcc catcttccag aatccgttcg aggcgttcag ccgctatcgt
     4921 acgattgact cttacctcta tgaaacggcg acgcgggtgg ccgggttgag caaggacgcg
     4981 gcagtgcagg cgctggagga ggctctggcg gtcgtcggac tcgaatacaa ggacgtgcgc
     5041 ggtaaatacc ccaaccagtt c
//
LOCUS       DASUYC010001068         3160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1568924, whole genome shotgun sequence.
ACCESSION   DASUYC010001068 DASUYC010000000
VERSION     DASUYC010001068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1568924"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..991)
                     /locus_tag="VF276_17440"
     CDS             complement(200..991)
                     /locus_tag="VF276_17440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX6608686.1"
                     /translation="MAHDPSGTPPVPLSPPLTPMVDPALLRPAPGSGIPDDYLVSIVM
                     PVYNEARTIRQIVEQVNAVPLKKELIAVDDCSTDGTGDVLEQLAREGQLRLFKHRVNG
                     GKGAAVRTGLAQVRGDIVIIQDADLEYDPAEYPLLIAPILKGRSKAVYGSRFLGPHKA
                     MYFWHQVGNKFLTLVTNVLFDTTLTDMETCYKVFTGDIARRLDIKSARWGIDPEITAK
                     ILRMGVRIYEVPISYTGREMWEGKKISWKDGIAVVGTLLRYRFLK"
     gene            complement(1045..1413)
                     /locus_tag="VF276_17445"
     CDS             complement(1045..1413)
                     /locus_tag="VF276_17445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608687.1"
                     /translation="MQQWEYLGVRLIPEADGGRVTVLDPLSLKGTPLNALGREGWEVV
                     GIFRDNFWVLLKRPLGTAPAQGTAPAAQAAPPAEPPASANLSPTYGVPDESRERALEQ
                     KLQQELQRRQHGGGDRPGRR"
     gene            1570..>3160
                     /locus_tag="VF276_17450"
     CDS             1570..>3160
                     /locus_tag="VF276_17450"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX6608688.1"
                     /translation="MTTLHIRLFGGLLLQRDQQTLPAPTPQKAALLLAYLLTAHNRTH
                     PRALLAGLFWGDQDERRARRNLSDALWRIRTLLEPDRDTAAPVLLFEGDTVRFNPDAP
                     VTHDVRAFEDLLRDPQDIEHQARAADLYRGDLLVGYYDDWVLLERERLRQMYLACLTR
                     LVSAYKTQGAWDEAVAAAGRLVQADPYQEDATRDLMRLYYRLGRRDRALQQFATLKAT
                     LANDLELEPEPETVALYQMMQAGMAAATLDAPPPVPAPTPVVRPAPTRPATTSALSLL
                     GNTTRSLRVVGRVREQADLAAWLASPRPQPPMLLLLGEAGVGKTRLAQDTAEEAYRHR
                     MFVLWGHYYQLAAPLPYAGLVEALRVGLRLGGVPALDPLWLSEVSRLIPELAEQHPGL
                     PRPVSLPPDQERIRLWEAFTQYLLALAAAGPHMFVLEDVQWTDSATLDLLQYALPRLR
                     NVGVGLRLLATARSEELANVPQVEAALRSLEAQNMLGYLPVDRLSPDAITELVQEALG
                     LDNPAPRFSSHLWRETEGNPFF"
BASE COUNT          464 a         1106 c         1084 g          506 t
ORIGIN      
        1 cgcatcacgg cctcgacggt gccggccatg cggttgatga cggcgtcggg cccctcctcg
       61 gcgcgagtct gttcccgcgc ctgggccagc acctggccgc gcaaatcaaa gcgggccgtg
      121 aggatcttgg tgccgcccag gtcgatgccg atcacatact cgtccatagg cccctcgtgc
      181 cggcggcgcg cgccgtcgcc tacttcaaga agcgatagcg cagcagggtg ccgacgacgg
      241 cgatgccgtc tttccaggag atcttcttgc cttcccacat ctcgcgcccg gtatacgaga
      301 tgggcacttc gtagatgcgc acgcccatgc gcaggatctt ggccgtgatc tccgggtcga
      361 tgccccagcg cgccgatttg atgtccaggc gccgggcgat gtcgccggtg aacaccttgt
      421 agcaggtttc catatcggtc agcgtggtgt cgaataggac gttcgtcacc agggtcagga
      481 acttgttgcc gacctggtgc cagaaataca tcgccttatg cggccccagg aagcgcgagc
      541 cgtagacggc cttgctgcgc cccttgagga tgggtgcgat gagcagcggg tactcggccg
      601 ggtcgtactc cagatcggcg tcctgaatga tgacgatgtc gccgcgcacc tgggccagcc
      661 cggtgcgcac cgcggcgccc ttcccgccgt tgacgcggtg cttgaagagc cggagctggc
      721 cttcgcgggc cagttgctcc agcacatccc ccgtgccgtc ggtcgagcag tcgtccacgg
      781 cgatgagctc cttcttcagg ggcaccgcgt tgacttgctc aacgatctgg cgaatggtgc
      841 gggcctcgtt gtagaccggc atgacgatgg agaccagata atcgtcgggg ataccgctgc
      901 cgggcgccgg gcggagcagg gccgggtcca ccatgggggt gagcggcggc gaaagcggga
      961 cgggcggcgt gccgctcggg tcatgtgcca tgctgtctac aatacctccg tgttaggctg
     1021 atgccgggcg caacgcgccg gggcttagcg gcgaccgggc cgatccccgc cgccgtgttg
     1081 ccggcgctgc aattcctgtt gtagcttctg ttccagggcg cgctcccggc tctcgtcggg
     1141 cacgccgtag gtgggcgaga gattcgcgga cgcgggtggc tccgcgggcg gcgcggcctg
     1201 ggccgcgggg gccgtgccct gggcgggcgc cgtgcccagc gggcgcttca gcagcaccca
     1261 gaagttgtcg cggaagatgc cgaccacctc ccagccctcg cgccccaggg cgttgagcgg
     1321 ggtgcctttc agactgagcg ggtcgagtac ggtgacccgg cccccatcgg cctccggaat
     1381 caagcggacg cccaaatatt cccactgttg catacggcga ttcctcctcc gtcaaaacgc
     1441 ctcattctac catagagcgc gggccgggtt caaccgtggc gccgggcggg ccggcgagat
     1501 ggcctcgcgg gggtctggga aataaacgct ccggcttatg ctataatacg ggggctgagg
     1561 aagtactgca tgaccacttt gcacattcgc ctgttcggcg gcctcttgct gcaacgcgac
     1621 cagcagacct tgccggcgcc cacgccgcag aaagccgcgc tgctcctggc gtacctgctc
     1681 accgcgcaca atcgcaccca cccgcgggcg ttgctggccg gcctcttctg gggcgaccag
     1741 gacgagcgcc gcgcccgccg caacctgagc gacgcgctct ggcgcatccg cacgttgctg
     1801 gagcccgacc gggacacggc ggccccggtg ctgttgtttg agggcgatac cgtgcgcttc
     1861 aaccccgacg cccccgtcac ccacgacgtg cgcgcgttcg aggatttgct gcgcgacccg
     1921 caggatatcg agcaccaggc gcgcgccgcc gacctctacc gcggcgatct gctggtcggc
     1981 tactacgacg actgggtgct gctggagcgc gagcgcctgc gccagatgta cctggcctgc
     2041 ctgacccgcc tggtgagcgc ctacaagacg cagggcgcct gggacgaggc cgtggcggcg
     2101 gcggggcgcc tggtccaggc cgacccgtac caggaagacg ccacgcgcga tctgatgcgc
     2161 ctgtattacc gccttggccg gcgcgaccgc gccctgcaac agttcgccac gctgaaagcc
     2221 acgctggcga acgacctgga actggaaccg gagccggaga cggtggccct gtaccagatg
     2281 atgcaggcgg gcatggcggc ggccacgctc gacgccccgc cgccggtccc ggcccccacg
     2341 cccgtggtcc ggcccgcgcc cacccgcccg gccaccacca gcgccctgag cttgctcggc
     2401 aacaccacgc gcagcctgcg cgtcgtcggg cgggtgcgcg agcaggccga cctggccgcc
     2461 tggctggcta gcccgcggcc tcagccgccc atgctgctgt tgctcggtga ggccggcgtg
     2521 ggcaagacgc gcctcgcgca agacaccgcc gaggaggcgt atcgccaccg catgttcgtg
     2581 ctctggggcc actattacca gttggcggcg ccgctgccct acgccgggct ggtcgaggcg
     2641 ctgcgcgtcg ggttgcgcct gggcggggtg ccggcgctgg acccgctctg gctgagcgag
     2701 gtcagccgcc tgatccccga actggccgag caacaccccg gcctgccgcg gcccgtcagc
     2761 ctgccgcccg atcaggagcg catccggctg tgggaagcct tcacgcagta cctgctggcg
     2821 ctggccgccg ccgggccgca tatgttcgtg ctcgaagatg tgcagtggac cgactccgcc
     2881 acgctcgacc tgctgcaata cgcgctgccg cgcctgcgca acgtcggcgt ggggctgcgc
     2941 ctgctggcga ccgcgcgcag cgaggaactg gcgaatgtgc cgcaggtcga ggccgcgcta
     3001 cgcagcctgg aagcgcagaa catgctcggc tatctgccgg tggaccggct ctcgcccgat
     3061 gccattactg aactggtaca ggaagcgctg gggctggaca atcccgcgcc gcgcttcagc
     3121 agccacctct ggcgcgagac cgagggcaac ccgtttttcg
//
LOCUS       DASUYC010001069         2888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_876930,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001069 DASUYC010000000
VERSION     DASUYC010001069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876930"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2021
                     /locus_tag="VF276_17455"
     CDS             <1..2021
                     /locus_tag="VF276_17455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608689.1"
                     /translation="EETDVAVREVFAPRAGIEPKGAPPDEAAAPANGDRRVLQLRPRR
                     PAPPASPSEAAESDTSHPPTQPISIHSVAATAHDFDAALGAPPAEHEDGERGLSPDEA
                     EPAGPSAAREGLDKLIGRARLFGRKAAPVVGTAGKATLGAAGTMLGHTLPENVRQRGA
                     AGMLNPDGVLEIEEVDEEPDAPPATEEEYEPEYAAEANRIVDFDAPRHAPAAPAPPFP
                     WMRWLVPIAVVALLGVLLFAVQQILAGQQTARIDVLLQQAQREESDGQDGTAAVRRDH
                     LLKALNQVQTALDIDAKSQPALRLESRIQSELDSLNGVVRLSGLNLLLDLSTARPATG
                     TTTAAPALPTDTPEVGATPSVTDSTALAPLIAPPKPGDYFSQVVVNGDDAFLLDKGSG
                     RLYRYRMAASQYSPILGAGDQVELVGTAGEKATVGALLFMAWRPTTEGGDLIALDDAR
                     VAYIWTPATGQWQAFRLGGADKLDRPRDLGTYDGNLYLLGTKPGQISKWAAGAYNNDP
                     TDWMSEAASNEIRNRGPVAMTIDGDIHLLLGDGRIVTMSAGEIKNTIALNVWPQVASP
                     LAIFTTETAHSLYVVEVADKRIIRVDKAGGAVQGQLKAPADSTAFDGLRNIYVDEAAG
                     KLYALSGKKLYVATLPPLPTAPGTPAPALPAADATPTAAPAVTATAAP"
     gene            2037..>2888
                     /locus_tag="VF276_17460"
     CDS             2037..>2888
                     /locus_tag="VF276_17460"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX6608690.1"
                     /translation="MSDLTGKTLGRYRILERIGRGGMAEVYKGYQPSLDRYVAVKVLH
                     PFLLEEEGSRARFEREARAVAALRHPNIVQVFDFDEQDGTYFMVMEYIAGPNLKAILQ
                     DRARQGARLSLARSGEIVTGIGGALAYAHRQGMIHRDVKPHNIMFAAEGQPLLTDFGI
                     AKIVGGENVSASGVLSGTPAYMSPEQGRALPLDSRTDIYSLGVVLYEMVTGRIPFDAD
                     TPFAVVVRHINDPLPLPRTLDPRIPEAVERVILKAMAKAPEERFQTADEMVRATQAAI
                     APLAATGA"
BASE COUNT          484 a         1080 c          947 g          377 t
ORIGIN      
        1 cggaagagac ggatgtggcc gtgcgcgagg tgttcgcgcc acgcgccggg atcgagccca
       61 aaggcgcccc gccggacgag gctgccgcgc ccgcgaatgg cgaccgccgc gtgctgcaat
      121 tgcgcccccg ccgcccggcg ccgcccgcct ctccgtcgga ggcggcggaa tccgacacat
      181 cgcatccgcc cacccagccc atctccatcc acagcgtggc ggccacggcc catgatttcg
      241 acgccgccct cggcgcgccc cccgccgagc acgaagacgg cgagcggggc ctgtcgccgg
      301 acgaagccga gccggcgggc ccgtccgccg cccgcgaggg cctggacaaa ctcatcgggc
      361 gggcgcggct gttcgggcgg aaagccgccc ccgtggtcgg cacggcgggc aaagccaccc
      421 tgggcgccgc gggcacgatg ctgggccaca cgctgcccga aaacgtgcgc cagcgcggcg
      481 ctgcgggcat gttgaacccc gacggcgtgc tggaaatcga ggaagtcgac gaagagccgg
      541 acgcgccccc ggccaccgag gaagagtacg aacccgaata cgcggcggaa gccaaccgga
      601 ttgtcgactt cgacgcgccg cggcacgccc cggccgcgcc cgcgccgccc ttcccctgga
      661 tgcgctggct ggtgcccatc gccgtggttg ctttgctcgg cgtgctgctc ttcgccgtgc
      721 agcagatcct ggccggccag cagacggcgc gcatcgacgt gctgttgcag caggcccagc
      781 gcgaagaatc cgacggccag gacggcaccg ccgccgtccg gcgcgaccac ctgctcaagg
      841 cgctgaacca ggtgcagacg gcgctggaca tcgacgcgaa gtcccaaccc gccttgcgcc
      901 tcgaatcgcg catccagagc gaactggaca gcctgaacgg cgtggtccgc ctgtcgggcc
      961 tgaacctgct gctcgatctg agcacggccc gcccggccac cggcacgacc accgccgcgc
     1021 ccgccctgcc gaccgatacg cccgaagtgg gcgccacgcc ctcggtcacg gacagcaccg
     1081 ccctggcccc gctgatcgcc ccgcccaagc ccggcgacta tttcagccag gtggtggtca
     1141 acggcgacga cgctttcctg ctggacaaag gcagcgggcg gctctatcgc taccgcatgg
     1201 cggcgagcca gtacagcccc atcctggggg cgggcgacca ggtcgaactg gtcggcaccg
     1261 ccggcgagaa ggccaccgtg ggcgcgctgc tgttcatggc ctggcggccc accaccgagg
     1321 gcggcgacct gatcgccctg gacgatgccc gcgtggccta tatctggacc ccggccaccg
     1381 ggcaatggca ggcgttccgc ctgggcggcg ccgacaaact cgaccggccc cgcgacctgg
     1441 gcacctacga cggcaacctc tatctgctcg gcaccaagcc cggccagatc agcaaatggg
     1501 cggcgggcgc gtacaacaac gatccgacgg attggatgag cgaggccgcc agcaacgaga
     1561 tccgcaaccg gggccccgtg gccatgacga ttgatggcga catccacctg ctgctgggcg
     1621 acgggcgcat cgtcaccatg agcgcgggcg agatcaagaa caccatcgcc ctgaacgtct
     1681 ggccgcaggt tgccagcccg ctggcgatct tcaccaccga gactgcccat tcgctttacg
     1741 ttgtcgaagt ggccgacaaa cgcatcatcc gcgtcgataa ggccggcggc gcggtgcagg
     1801 ggcagttgaa agcccccgcc gacagcacgg cctttgacgg cctgcgcaac atctacgtgg
     1861 acgaagcggc gggcaaactc tatgccttaa gcggcaaaaa gctctacgtc gcgacgttgc
     1921 ccccgctacc caccgcgccg ggcacacccg cgcccgcgtt gccggccgcc gatgctaccc
     1981 cgaccgccgc gcccgccgtc accgccaccg ccgcacccta gcggctgaga accgccatga
     2041 gtgacctgac cgggaagacc ctcggccgct accggattct tgaacggatc gggcggggcg
     2101 gcatggctga ggtctataaa ggctaccagc cctcgctcga tcgctacgtg gcggtgaagg
     2161 tgctgcatcc cttcttgctc gaagaagaag gtagccgtgc ccgcttcgag cgcgaggcgc
     2221 gcgccgtggc cgccctgcgc caccccaaca tcgtccaggt cttcgacttc gacgagcaag
     2281 acggcaccta tttcatggtc atggagtaca tcgccgggcc gaacctgaag gccattttgc
     2341 aggatcgggc ccgccagggc gcccgcctca gcctggctcg cagcggcgag atcgtcaccg
     2401 gcatcggcgg cgccctggcc tacgcccacc gccagggcat gatccaccgc gatgtcaagc
     2461 cccacaacat catgttcgcc gccgagggcc agccgctgct gaccgacttc gggatcgcca
     2521 agatcgtcgg cggcgagaac gtcagcgcca gcggcgtgct gtcgggcacc cccgcctata
     2581 tgagcccgga acagggccgc gcgctgccgc tggactcgcg caccgatatc tactcgctcg
     2641 gcgtggtgct ctacgagatg gtgaccgggc gcatcccctt cgacgccgat acccccttcg
     2701 ccgtggtggt ccggcacatc aacgacccgc tgccgctgcc gcgcaccctc gatccccgga
     2761 ttcccgaagc cgtggagcgg gtgatcctca aggcgatggc gaaagcgccg gaggagcgtt
     2821 tccagacggc ggacgagatg gttcgggcga cgcaggcggc gattgccccg ctcgccgcca
     2881 cgggcgcc
//
LOCUS       DASUYC010001070         2358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1336156, whole genome shotgun sequence.
ACCESSION   DASUYC010001070 DASUYC010000000
VERSION     DASUYC010001070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1336156"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VF276_17465"
     CDS             complement(<1..188)
                     /locus_tag="VF276_17465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608691.1"
                     /translation="MAVDVGAILSRAFQVTFKNPVLWLLGFLVAVLSGTSNTNYRFNA
                     ADFMPDSVLGSSILALVIL"
     gene            complement(337..1731)
                     /locus_tag="VF276_17470"
     CDS             complement(337..1731)
                     /locus_tag="VF276_17470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608692.1"
                     /translation="MAIDFGALVSRTFQILLRYKVLWVLGVIVTLLSTNLNFSYNANS
                     FNRFNTASEPDVAALAGGAGLLACVAFILYVIFFFVRAGFEAGLIIAGDRAAQDRPPT
                     LQEAWALGRTRMWQIIGLNLIIVAIGLVVALIFVILALVILGSAVAGIFSSIASSTGQ
                     PADPSTIFPALGGFFVCFCGFLLLAIPLFALLSVVVQLAQRAAVLDNQGVGQAWSTGW
                     QLFRANIGNVLMLLLIEFGIGILVAIVIGVISAAVSLPLTALALSGGTGAGGTLLGIV
                     IGVILWVVTGVLGALPLAGNSVLWTLFYRILTVPAGAGPGQAALYPGGPPPGAYGAPP
                     ASPYGTPGGYSQPGPPAGGYSQPGQYPGGYTQPGQYPGGYSQPPGGYTQPGQYPGGYV
                     PPSGASGGYGAPAPPASGPGQPPGGYPPPAPPANPYGPPAGQGGYSPPPGGGEDRARQ
                     EGGLERPEDTPPRT"
     gene            complement(1736..>2358)
                     /locus_tag="VF276_17475"
     CDS             complement(1736..>2358)
                     /locus_tag="VF276_17475"
                     /inference="COORDINATES: protein motif:HMM:NF037737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608693.1"
                     /translation="KSDGVHLRLTARAADAAAARALLAPLEAAVRERLGDAIWGADAD
                     TLEDVVCNLLAQFDTHLAVAEIGGATGGQIARVLSSATAVDRVTAVLDVPSPEAHAVA
                     QAGLDSGGTDPLSEEGVRAVAAAVALRWNTGGLLVTAGRLPAGRDDERVLGEMWVAWS
                     CAAPGHVGTVKTLRLPVRAARGEVKRLMALQALNLLRKELAAIGVK"
BASE COUNT          410 a          823 c          804 g          321 t
ORIGIN      
        1 agaatcacca gggccaggat gctactgccc aatacggaat cgggcataaa atcggcggca
       61 ttgaaccgat aatttgtgtt ggaagtgcct gatagcacgg ccaccagaaa ccccagcagc
      121 cagagtaccg ggtttttgaa ggtgacctgg aaagcgcgcg atagaatcgc gcccacatcg
      181 accgccatgg ctgacctcct gtgcgctaaa gaccccggac ccgctcaaga tccctgcttg
      241 gatggcattc tccgcagaca gagtccggca ttctaattgt cgcacagccg gcgcgccata
      301 gcggttacaa cctgataacc cggcgataca gcctgctcac gtgcgtggcg gggtatcctc
      361 tggccgctcc aggccgcctt cctggcgggc gcgatcctcg ccgccacccg gcggcgggct
      421 gtagccgccc tggccggcgg gcgggccata ggggttcgcg ggaggcgccg ggggcgggta
      481 gccgccgggc ggctggccgg ggccggacgc gggcggagcc ggtgcaccat accccccgga
      541 ggcgccggac ggcgggacat agccacccgg atactggccc ggctgggtgt agccgcctgg
      601 tggctggcta tagccaccgg gatattgccc cggctgggta tacccgccgg gatactggcc
      661 gggttggctg tagccgcctg cgggcgggcc gggttggctg tagccgccgg gggtcccata
      721 cgggcttgcc ggcggcgcgc catacgcacc aggcggcggg ccgccgggat agagcgcggc
      781 ctgccccggt cccgcgcccg cgggcacggt gaggatgcgg tagaacagcg tccagagcac
      841 cgagttgccg gcgaggggca gggcgccgag cacccccgtc accacccaga ggatgacccc
      901 gatgacgatg ccgagcagcg tcccgccggc gccggtgccg ccgcttaggg ccagagccgt
      961 cagcggcagc gacacggcgg cgctgatcac cccgatgaca atggcgacca ggatgccgat
     1021 gccgaactcg atcagcagca gcatcagcac attgccgatg ttcgcccgga acaactgcca
     1081 gcctgtgctc cacgcctgac ccacgccctg gttatccagc accgcggcgc gctgggccaa
     1141 ctggacgacc acactcagta gcgcgaagag cggaatcgcc agcagcaaga agccgcagaa
     1201 gcagacaaaa aacccgccca gcgccgggaa gatggtcgaa gggtcggcgg gttgcccggt
     1261 ggacgacgca atgctgctga agatcccggc aaccgcgctg ccgaggatga ccagggccag
     1321 gatcacgaaa atgagggcga ccacgagccc gatggcgacg ataatcaggt tcagcccgat
     1381 gatctgccac atgcgcgtgc ggcccagcgc ccaggcttcc tgcaacgtcg gcgggcggtc
     1441 ctgcgcggcg cggtcgccgg cgatgatcag cccggcctcg aacccggcgc gtacgaagaa
     1501 gaagatgacg tacaggatga acgccacgca ggccagcagg ccggcgccgc cggccagcgc
     1561 ggccacatcc ggctcgctcg ccgtgttgaa ccggttgaag ctgttggcgt tgtagctgaa
     1621 gttgaggttc gtgctcagca gcgtcacgat cacgccgagc acccacagca ctttgtagcg
     1681 caagaggatt tggaaggtgc gggacaccag ggcgccaaaa tcgattgcca tatgcttact
     1741 tcactcctat ggctgccagt tccttacgca gcagattgag cgcctgtagc gccatcaacc
     1801 gcttgacctc gccccgcgcg gcgcggacgg gcaggcgcag ggtcttcaca gtgccgacat
     1861 ggcccggcgc ggcgcaactc caggccaccc acatttcgcc cagcacccgc tcgtcgtcgc
     1921 ggcccgcggg caagcgcccc gccgtgacca gcaggccgcc cgtgttccag cgaagggcca
     1981 cggcggcggc cacggcgcgc actccttcct cgctcagcgg gtccgtgccg ccgctatcca
     2041 gcccggcctg cgcgacggcg tgcgcttccg ggctgggcac atcgagcacc gccgtgaccc
     2101 gatccaccgc cgtggcgctg ctcagcacgc gggcgatctg cccgccggtg gcgccgccga
     2161 tctcggccac ggccaggtgc gtgtcgaact gcgccagcaa gttgcacacc acatcttcga
     2221 gcgtatcggc atcggcgccc cagatggcgt cgcccaggcg ttcgcgcacg gccgcctcca
     2281 gcggcgcgag caaggcccgc gcggcggcgg catcggcggc gcgcgccgtc agccgcaggt
     2341 ggacgccgtc gctcttgg
//
LOCUS       DASUYC010001071         2602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_2049493, whole genome shotgun sequence.
ACCESSION   DASUYC010001071 DASUYC010000000
VERSION     DASUYC010001071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2049493"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VF276_17480"
     CDS             complement(<1..262)
                     /locus_tag="VF276_17480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608694.1"
                     /translation="MERPVFRSRADYAGRFTDAAYWRPYIEAVCDRHALGPCNEVRAG
                     LPGTNAVFLVDGRYAVKLYTDLFGGATSFPAERDLYGWFAAHP"
     gene            complement(284..1297)
                     /locus_tag="VF276_17485"
     CDS             complement(284..1297)
                     /locus_tag="VF276_17485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013173477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HEX6608695.1"
                     /translation="MRDRKRIWVAPRTPWVATFSIVGQDPRNGDLGVAVQSKFLAVGA
                     VVPWARAGVGAVATQSWANTSYGPAALDLLAQGQEPQAVIDTLVANDEGRAQRQVGIV
                     DAQGRAATFTGSECFAWAGGRTGPHFAAQGNILVSQETVDSMAETFLEQQMSGEGELS
                     DHLVAALAAGQAAGGDSRGMQSAALLVVRAAGGYAGFNDRYIDLRVDDHPYPIAELRR
                     ILDLHQLYFQPTDPTSLLPLSGAALDEVRRLLHEGGYLPTVPTDESYDPVTQEAFSAF
                     AHRENLEDRWQEDARVDPVVLRYLRTHIAQMPRVESTPAEPPHLHIVRPSERPGSHAP
                     EVE"
     gene            complement(1437..>2602)
                     /locus_tag="VF276_17490"
     CDS             complement(1437..>2602)
                     /locus_tag="VF276_17490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608696.1"
                     /translation="GSAGMNRLSRPLLLTQVLLLGVLALGGLLAACDVPKPPAEALTP
                     VVGSATRTPKIIDPNVPTATPAPMTLPATGRLWFLRGGVLWTAAPDGSGAKAVSTNKA
                     TSPPVPNGAGTKVAYLSERKLVLLDVASGQETTLVEADMSPLQRPSWSPDDKQIAYFT
                     EDATQYGTEVVWAIPAAGGAPQHVTALADGGYRSGPTFEHVASWAGDGRRLAVSGPQG
                     PIHILPLDQTAGDPKVVNGGEPTWSTDSRTLLYTETLNGALALQDVVTDDFQPYRNEK
                     RLDGTRLGQYAQAPLPRFNADASLILYRAQGGDGAPAVAIRARPDGSEQLFLPGNNAG
                     WAPDGQWLVYETGTLVPTEAGKMWQPQGLARIRADGSGQTDVLSDASWPAWSK"
BASE COUNT          358 a          904 c          941 g          399 t
ORIGIN      
        1 cagggtgcgc ggcaaaccag ccgtagagat cgcgctcagc ggggaagctg gtcgcgcccc
       61 cgaacaggtc ggtgtagagc ttgaccgcgt agcggccatc cacaaggaag acggcgttcg
      121 tgcccggcag gcccgcgcgg acctcgttgc acggccccag ggcatggcgg tcgcacacag
      181 cctcgatata ggggcgccag taggcggcgt cggtaaagcg cccggcatag tcggcgcgcg
      241 aacggaagac cggcctttcc atcggttcgc gggcgcgccc ggcctactcg acttcgggcg
      301 cgtggctgcc ggggcgctcg ctgggccgca cgatatgcag gtgaggcggc tccgccgggg
      361 tgctttcgac gcgcggcatc tgcgcgatgt gcgtgcgcag gtagcgcagc accaccgggt
      421 cgacgcgcgc atcctcctgc caccggtctt ccaggttttc ccgatgggcg aaggcgctga
      481 aggcttcctg ggtcacggga tcgtagctct catcggtggg cacggtgggc aggtagccac
      541 cctcgtgcag caggcgccgg acctcgtcca gcgcggcccc gctcagcggc agcaggctgg
      601 ttgggtcggt gggctggaag tagagctggt gcaggtcgag gatgcggcgg agttcggcga
      661 tggggtaggg gtggtcgtcc acgcgcagat cgatgtagcg gtcgttgaag cctgcgtagc
      721 cgcctgccgc gcgcaccacc agcagcgccg ccgattgcat gccgcggctg tcgccgcccg
      781 ccgcctgccc agcggccagc gcggccacca ggtgatcgga tagctcgccc tcgccgctca
      841 tctgctgctc caggaaggtc tcggccatgg aatccacggt ctcctggctg accaggatgt
      901 tgccctgcgc ggcaaagtgc ggccccgtgc ggcccccggc ccaggcgaag cactcgctgc
      961 cggtgaaggt ggcggcgcgg ccctgggcat cgacgatgcc cacctggcgc tgggcgcggc
     1021 cctcgtcgtt ggcgaccagc gtgtcgatca cggcttgcgg ctcctggccc tgggccagca
     1081 gatcgagtgc cgccgggcca tagctcgtgt tggcccacga ctgggtcgcc acggccccga
     1141 ccccggcgcg cgcccacggc accaccgcgc ccacggccag gaacttcgat tggacggcca
     1201 cgcccaggtc gccgttgcgc gggtcctggc ccacaatcga gaaggtcgcc acccacggcg
     1261 tccgcggcgc cacccagatc cgcttgcggt ctcgcatctg ctcggtctcc ttatacccaa
     1321 tacgcgccgt actgcacgct atccgttcaa cgcgcgacct tctccaaccg gcactacgct
     1381 actcctcata ctgaccactg accaccgacc actgaccact cgtcgtcatt cagcactcac
     1441 ttgctccacg ccggccagga ggcgtcggag agcacgtccg tctggccgct gccgtcggcg
     1501 cggatgcggg cgaggccctg cggctgccac atcttgcccg cttcggtcgg caccagcgtg
     1561 ccggtctcgt agacgagcca ctggccgtcg ggcgcccagc cggcgttgtt gccgggcagg
     1621 aacagttgct ccgaaccgtc ggggcgggcg cggatcgcca cggcgggcgc gccgtcgccg
     1681 ccctgcgcgc ggtagaggat cagcgaggcg tcggcgttga agcgcggcaa gggcgcctgc
     1741 gcgtactggc ccaggcgggt gccgtcgagg cgcttctcgt tgcggtaggg ctggaaatcg
     1801 tcggtgacca cgtcttgcag ggccagggcg ccgttcagcg tctcggtata gagcagggtg
     1861 cggctgtcgg tcgaccaggt gggttcgccg ccgttcacga ccttggggtc acccgccgtc
     1921 tggtccaggg gcaggatgtg gatcggcccc tgcggcccgc tgaccgcgag gcggcggccg
     1981 tcgccggccc acgaggctac atgctcgaaa gtcggcccgc tgcgatagcc gccgtcggcc
     2041 agcgcggtca catgctgcgg cgcgccgccc gccgccggga tggcccagac gacctccgtg
     2101 ccgtactggg tggcgtcttc ggtgaaatag gcgatctgct tatcgtcggg cgaccaggac
     2161 gggcgctgga gcggcgacat gtcggcttcg accagcgtgg tctcctgccc gctcgccacg
     2221 tcgagcagaa ccaatttgcg ctcgctcagg taggccactt tggtgcccgc cccgttgggc
     2281 acgggcggcg aggtggcctt gttggtggac acggccttcg cgccgctgcc gtcgggcgcc
     2341 gccgtccaga ggacgccgcc gcgcaggaac cagagccgcc cggtggcggg cagggtcatc
     2401 ggcgccgggg tggcggtggg cacgttggga tcgatgatct tgggtgtgcg cgtggccgag
     2461 ccgaccaccg gggtcagcgc ctcggcgggg ggtttgggca catcgcaggc ggccagcagt
     2521 ccgccgaggg ccagcacacc cagcagtagc acctgggtca gcaacaaagg acgggacagg
     2581 cggttcatgc cggcactccc tc
//
LOCUS       DASUYC010001072         3417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_414309,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001072 DASUYC010000000
VERSION     DASUYC010001072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414309"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2216
                     /locus_tag="VF276_17495"
     CDS             <1..2216
                     /locus_tag="VF276_17495"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6608697.1"
                     /translation="TAAGGQARVVLLTGEVGIGKSRLWSEWAATLPPDEPVLETRCLE
                     ATQALPFAPLIELFSSRAYVSRLFTPPSPVPPIWLAEVARILPEVRTLAPDLPAPAVL
                     PAAEERRRLFEAFVQCVLALGGRPLVFFIDDLHWADNATLDWLGYLVHRMREAALLLV
                     AAYRPAEAPPALVHLLAGWGREGLTRRVALAPLTPAESVALVTELGGDPALAEQVQEQ
                     SAGNPYFLIELCQAAPGNVPPALAELVRARLDRLPETARQVAQAAAVLEPDFDFPTLR
                     RTSGRGEEETLDALDTLLEAGVLREVGGRYSFSHPFVATVVRDGLSGARRAFIHRRAA
                     EAREAEYADRLPVIAGRLAAHYEAAGDPARAATYAEMAAQRALALAAPAEAADFYRLA
                     LALEPTPDRQMGLGRTLLRQGDMAGAQAAYRAARAGFQAAGRRADMARAALSMAETYL
                     PAGRPDELLHWVELALPDLDADADPAAHARAHFLLGAGHLAREIDTAEAERHLSEAAE
                     LATANHLPDLAGQSRFELGNLRAQRGDLPGARRAYDEAIAFAQEAGDPFQEVLGHNNA
                     AYHALLAGDLEAAHRHLDTALAMSERGDLRMPRQYLYSTRGELALAEDRPAEAETWFR
                     RGLAEAESTGNSRQVANYHANLSLARRAQGDLDGALALLEQAQAEAAPLVAPYLQTQV
                     DLWRAELYTARGEYAAAEAALAQATARLANAGHPHLLAWADRLRRKAPGGAPAPC"
     gene            2210..3217
                     /locus_tag="VF276_17500"
     CDS             2210..3217
                     /locus_tag="VF276_17500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HEX6608698.1"
                     /translation="MLALPAALEAAIAAALKDVPASQWMSAARALSDRYRAERAGGER
                     ALARGAPAALGYAALILPAAYAQLRGAMTATAARIPGWRPTTVLDLGSGPGTALWAAL
                     EQWPSLGTFQAWEREQAFITLGQRLAATGPRPLARATWRQIALDGPLPADTPRFDLVV
                     LGHVLNELDEPVRQRLVAWAWDHCAGLLLIVEPGTSAAFPVVRAARDQLLATGARTIA
                     PCAHDAPCPLRGDWCHFPQRLERPAFQRRAKDASAGTEESKFSYAAMARFGTDPIWGR
                     LIHQPQIHKGFAELIVSSREGIVRPRVFKRERERYREVTDYRWGDALPAPVEPTLPTA
                     K"
BASE COUNT          442 a         1333 c         1196 g          446 t
ORIGIN      
        1 cgacggcggc cggggggcaa gcgcgtgtgg tgttgttgac cggggaagtc ggcatcggca
       61 agtcgcggct ctggagcgag tgggcggcga ccttgccgcc ggacgagccg gtgctggaga
      121 cgcgctgcct ggaagcgacg caggcgttgc ccttcgcccc gctgatcgag ctgttcagca
      181 gccgcgccta cgtgagccgc ctgttcacgc caccgtcgcc cgtgccgccg atctggctgg
      241 ccgaggtcgc gcgcatcctg cccgaggtcc gcaccctggc gccggatctg cccgcgcccg
      301 ccgtgctgcc cgccgccgag gagcgccgcc gcctcttcga ggcctttgtg cagtgcgtcc
      361 tggcgctggg cgggcgaccg ctggtcttct tcatcgacga cctgcactgg gccgacaacg
      421 ccacgctcga ctggctgggc tacctggtcc accgcatgcg cgaggccgcg ctgctgctgg
      481 tcgccgccta ccggcccgcc gaagcgccgc ccgcactggt gcacctgctc gccgggtggg
      541 gtcgcgaggg cctgacccgg cgcgtcgccc tggcgccgct cacgcccgcg gagtcggtcg
      601 ccctcgtcac cgagctgggc ggcgacccgg cgctggccga gcaggtgcag gagcagagcg
      661 cgggcaatcc ctatttcctg atcgaattgt gccaggccgc gcccggcaac gtgccgcccg
      721 cgctggccga actggtgcgc gcccgcctgg accgcctgcc ggaaacggcc cgccaggtgg
      781 cgcaggccgc cgccgtgctc gaacccgatt tcgacttccc caccctgcgc cgcaccagcg
      841 ggcggggcga ggaagagacg ctcgacgcac ttgacacgct actggaggca ggcgtgctgc
      901 gcgaagtggg cgggcgctac agcttcagcc atcccttcgt ggcgacggtg gtgcgcgacg
      961 ggttgagcgg cgcgcgccgg gcctttatcc atcgccgggc cgccgaggcg cgcgaagccg
     1021 agtacgcgga ccgtctgccg gtcatcgccg ggcggctggc cgcccattac gaggccgcgg
     1081 gcgacccggc ccgtgccgcc acctacgccg agatggccgc ccagcgcgcc ctggccctgg
     1141 cggcgcccgc cgaagccgcg gacttctacc ggctagcgct ggccctagag ccgacgcccg
     1201 accgccagat gggcctgggc cggacgctgt tgcgccaggg cgatatggcc ggggcgcagg
     1261 ccgcctatcg cgcggcgcgc gcgggcttcc aggccgcggg ccgccgcgcc gatatggccc
     1321 gcgccgccct gagcatggcc gagacctacc tgcccgcagg ccgcccggac gaactgctgc
     1381 actgggtcga actcgcccta cccgatctcg acgcggacgc cgacccggcg gcccatgccc
     1441 gcgcccactt cctgctgggg gccgggcacc tggcccgcga aatcgacacc gccgaagccg
     1501 agcgccacct gagcgaggcg gcggaactgg cgaccgccaa ccacctgccc gacctggccg
     1561 gtcagagccg ctttgaactg ggcaacctgc gcgcccagcg tggcgacctg ccgggcgcgc
     1621 gccgggccta tgacgaggcg attgccttcg cccaggaggc gggcgacccc ttccaggagg
     1681 tgctgggcca caataacgcc gcgtatcatg cgctgctggc gggcgacctg gaggccgcgc
     1741 accgccatct cgacaccgcg ctggcgatgt ccgagcgggg cgacctgcgg atgccgcgcc
     1801 agtacctcta cagcacgcgc ggcgaactgg ccctggccga ggaccggccc gcggaggccg
     1861 aaacctggtt ccgccgcggg ctggccgaag ccgagtccac cggcaacagc cgtcaggtcg
     1921 ccaactacca cgccaacctg agcctggccc gccgggcgca gggcgatctc gacggtgcgc
     1981 tggctctgct cgaacaggcc caggccgagg cggcgccgct ggtcgcgccg tatctgcaaa
     2041 cgcaggtcga tctctggcgg gccgagctct acacggcgcg cggcgaatat gccgccgccg
     2101 aggcggcgct ggcccaggcg acggcgcggc tagcgaacgc cgggcacccg cacctgctgg
     2161 cctgggccga ccggctgcgg cgcaaggcgc cgggcggagc gccggcccca tgctagccct
     2221 ccccgccgcc ctggaagcgg ccatcgccgc ggcgctgaag gacgtgcccg cctcgcagtg
     2281 gatgagcgcg gcgcgcgccc tgagcgaccg ctaccgcgcc gagcgggcgg gcggcgaacg
     2341 cgcattggcc cgcggggcac ccgccgcgct cggctacgcg gccctgatcc tgcccgccgc
     2401 ctacgcgcaa ttgcgcggcg ccatgaccgc caccgccgcc cgcatccccg gctggcgccc
     2461 taccaccgtg ctcgacctgg gcagcgggcc gggcaccgcg ctctgggcgg ccctggagca
     2521 gtggccgtcg ctgggcactt tccaggcgtg ggagcgcgag caggcgttca tcaccctggg
     2581 gcaacgactc gctgccaccg gcccacgccc gctggcccgc gccacctggc ggcagatcgc
     2641 gctcgacggc ccgctgcccg ccgatacgcc ccgctttgac ctggtggtcc tcggccatgt
     2701 gctcaacgaa ctcgacgagc cggtgcggca gcggttggtc gcctgggcct gggaccactg
     2761 cgcgggcctg ctgctgatcg tggagccggg cacctcggcg gccttccccg tcgtgcgcgc
     2821 ggcccgcgac cagttgctgg cgaccggggc gcgaaccatc gccccttgcg cccacgacgc
     2881 cccctgcccc ctgcgcggcg actggtgcca tttcccgcag cggctggagc gccctgcgtt
     2941 ccagcgccgg gccaaggatg cctccgcggg taccgaggag agcaagttct cctacgccgc
     3001 catggcccgc ttcggcaccg accccatctg gggccggctc atccaccagc cgcagatcca
     3061 caagggcttc gccgaactca tcgtctccag ccgcgaaggc atcgtgcgcc cgcgcgtctt
     3121 caagcgcgag cgcgagcgct accgcgaggt caccgactac cgctggggcg acgccctgcc
     3181 cgccccggtc gaacccaccc tgcccaccgc caagtagcgc gtcggcggcc cctgtcattc
     3241 tgagcgcagc gaagaatctc gtctcctgcg gacgaagaga ctcttcgctg cgctcttctg
     3301 aaaatactat ccggccctgg tcaggcggct tggtccttgg gtttgagcac gacttcgttc
     3361 cccaagtgct ccaagcgccg cacgagctgc cgttccagca gcgcccggtc acgctcg
//
LOCUS       DASUYC010001073         3129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1604793, whole genome shotgun sequence.
ACCESSION   DASUYC010001073 DASUYC010000000
VERSION     DASUYC010001073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1604793"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="VF276_17505"
     CDS             complement(<1..1122)
                     /locus_tag="VF276_17505"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6608699.1"
                     /translation="MRAKPKHLGPEYAAQFADASVASAYHHRIPYPPEVFAILAGLLG
                     DGPRVVLDVGCGTGEIARPLLPLVDRVDAVDPSAAMIARGRALPGGDDPRLRWIVGRA
                     EDAPLAPPYGLITAGSSIHWLDWDVALPRFRRLLAPGGYLALLSPAARPLPWAAELQR
                     IIDRYSTNRDYAPYDIVEELTSRGLFHVRGTQTTAPIAFSQPLADYIESYHAMNGFSR
                     DRMTAEAAAGFDAAVRALVAPFCPDGQVRLEVTATVTWGVPGRIMVNADMSFRSLKPD
                     DYPLLLEWLQRPHVKEWWDDGDDTLDKVAAHYSDPDAERFILLYQEAGSMDEARPIGY
                     FQYYREDAGVVGIDQFIGEADLLNRGLGTLAVRAFIALVI"
     gene            complement(1218..2540)
                     /locus_tag="VF276_17510"
     CDS             complement(1218..2540)
                     /locus_tag="VF276_17510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608700.1"
                     /translation="MGRLTPFGSLVLLILLIAGVGGLLAMTDNLRWPPFGITLTAPTP
                     IVITATPNPSGPPAATSVALVPTNPPAAPNMTAAPTAPPPTATSTPTATPTATSTPTP
                     TPPGLAYVGAIDVPGLDAGPGGPQPAATDPETHRLYVMTTNGAGRGILGVYDETNLQK
                     QKDIPLDLEGMTLNTVDPVPVLVHGPTKRIYVVAPNGRIAVIDSNALAETRSFNLGQP
                     IEHAFLNPDGTKLYGLLSDQPGSGGPLLRMLDLQKVTTKGIKIPSGWSADSAFVDGTT
                     LYLTGREGLLPMDMRYDIFGGPIQLGFEPLFPLFDGSARRLFAWRQPQTVPLVPEMVV
                     LDAGNWQVAAKLTHDGCPCLLAYGGGRQRVYLAVPQSPGRVLAYSLGDKLATHDDLVL
                     NTVPALNILPSASAPRLYVQSSGPHAILVLQDNGPAPGRQPTVVDVTK"
     gene            complement(2556..2993)
                     /locus_tag="VF276_17515"
     CDS             complement(2556..2993)
                     /locus_tag="VF276_17515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608701.1"
                     /translation="MVVRFVIYGLLGWCMEIVWTALPKRRPIDWRLPGHTYLWMFLIY
                     GLVAPLYEPLHDGLRTAGVAWPLRGLLYAAGIMGVELVTGWLLERLTGRCPWDYSATA
                     RWHWRGYTRFDYAPLWFLVGLALEPLHDFLVRLTPAITAALAL"
BASE COUNT          503 a         1082 c         1086 g          458 t
ORIGIN      
        1 gatcaccaac gcgatgaacg cgcgcaccgc cagcgtgccc aggccgcgat tgagcaggtc
       61 ggcttccccg ataaactggt cgatgcccac cacgcccgcg tcttcccggt aatactggaa
      121 gtagccgatg ggccgggcct cgtccatgct tccggcttcc tggtagagga gaatgaagcg
      181 ctcggcgtcc gggtcgctgt agtgggcggc caccttgtcc agcgtgtcgt cgccgtcatc
      241 ccaccactcc ttgacgtgag gccgctgcaa ccattcgagc aagagggggt aatcgtccgg
      301 cttcagcgat cggaaactca tgtcggcatt gaccattatg cgtcccggca caccccaggt
      361 gacggtcgcc gtgacctcca ggcgcacctg gccgtcgggg cagaacggcg ccaccagggc
      421 acgcaccgcc gcgtcgaagc ccgcggcagc ctccgccgtc atccggtcgc gcgaaaagcc
      481 gttcatggcg tggtacgatt cgatatagtc cgcgaggggc tggctgaagg cgatgggcgc
      541 ggtcgtctgc gttccgcgca cgtggaacag cccgcgcgac gtgagttctt ccacaatatc
      601 gtagggcgcg tagtcacggt tggtggagta gcggtcgatg atgcgctgta gctcggcggc
      661 ccagggcagc gggcgggcgg ccggcgagag cagcgcgagg tagccacccg gcgccagcag
      721 gcggcggaac cggggcagcg ccacgtccca atccagccag tggatgctcg accccgccgt
      781 gatcagccca tagggcgggg ccagcggcgc gtcctcggcc cgcccgacaa tccagcgcag
      841 gcgcggatca tccccgcccg gcagcgcccg gccccgcgcg atcatggcgg cggacggatc
      901 gacggcatcg acccggtcca ccagcggcag cagcgggcgg gcgatctcgc cggtgccgca
      961 gcccacgtcg agcaccacgc gcgggccgtc gcccagcagc cccgccagga tggcgaacac
     1021 ctccggcggg taggggatgc gatggtggta ggcgctcgcc acgctcgcgt cggcgaactg
     1081 tgccgcgtat tccggtccca ggtgtttcgg cttggcgcgc atcggtacct cctttgccca
     1141 ggttcggcag gcacaggtag gggcgggtgt gacacccgcc ccgttgcccg tatctctata
     1201 gtcgcccgcg cccgatccta cttggtcaca tcgaccacgg tgggctggcg accgggggcc
     1261 ggaccgttgt cttgcaacac caggatggcg tgcggcccgc tgctctgcac atagagccgc
     1321 ggcgcgctcg cgctgggcag gatgttcagc gcgggcaccg tgttcagcac gaggtcgtcg
     1381 tgcgttgcca gcttatcgcc gagcgagtag gccagcaccc ggcccggcga ttgcggcacg
     1441 gccaggtaga cgcgctgccg cccgccgccg taagccagca agcaggggca cccgtcgtgc
     1501 gtcagcttgg ccgccacctg ccagttgccc gcgtcgagca cgaccatctc cggcaccagc
     1561 ggcaccgttt gcggctggcg ccaggcgaac agccgccggg cactgccgtc gaacagcggg
     1621 aacaacggct cgaacccgag ctggatcggc ccgccgaaga tatcgtagcg catgtccatc
     1681 ggcaacaggc cctcgcgccc ggtgaggtag agggtcgtgc cgtccacgaa cgcgctgtcg
     1741 gcggaccacc cgctggggat cttgatcccc ttcgtcgtca ccttctgcag gtcgagcata
     1801 cgcagcaacg ggcccccgct gcccggctgg tcgctcaaca agccgtacag cttggtgccg
     1861 tcggggttca ggaaagcgtg ctcgatgggc tgccccagat tgaaggaccg cgtctcggcc
     1921 agggcgttgc tgtcgataac cgcgatgcgc ccgttgggcg ccaccacgta gatccgcttg
     1981 gtggggccat gcaccagcac gggcaccggg tccaccgtgt tcagcgtcat gccttccagg
     2041 tccagcggga tgtccttttg cttttgcagg ttcgtctcgt cgtagacgcc gaggatgccc
     2101 cggcccgcgc cgttggtggt catgacgtag agccggtggg tctccgggtc cgtggcggcc
     2161 ggttgcggcc cgccgggacc ggcgtcgagg cccggcacgt cgatggcgcc gacataggcc
     2221 aggcccggcg gcgtcggcgt cggcgtgctg gtggccgtcg gcgtcgcggt cggcgtgctg
     2281 gtggccgtcg gcgggggtgc cgtcggcgca gcggtcatgt tgggggcggc gggcgggttg
     2341 gtcggcacca gggcgacgct ggtggcggcg ggcggcccgc tcggattcgg ggtggccgtg
     2401 atcacaatcg gggtgggcgc agtcagcgtg atgccgaacg gcggccagcg cagattgtcg
     2461 gtcatcgcca gcaggccacc cacgccggcg atgagcagga tcagcaagac cagtgagcca
     2521 aaaggtgtta gtctacccat aaacagaagg accctctaga gtgccagcgc cgcggtgatc
     2581 gccggagtga ggcgcaccag gaagtcgtgc aacggctcca gggccaaccc caccaggaac
     2641 cagagcggcg cgtaatcgaa gcgcgtatag ccgcgccagt gccagcgcgc cgtggcgctg
     2701 tagtcccacg gacaacgacc ggtcaggcgc tccaacagcc agccggtcac cagctccacg
     2761 cccatgatac ccgcggcata cagcagcccg cgcaacggcc aggccacgcc cgccgtgcgc
     2821 aggccatcgt gcagcggctc atagagcggc gccaccaggc cataaatcag aaacatccag
     2881 aggtacgtat ggcccggcaa ccgccagtca atggggcggc gcttgggcag ggccgtccag
     2941 acgatctcca tgcaccagcc cagcaggcca taaatcacga agcgcacgac catccgttgc
     3001 gtacctctcc ggggaggctg ccccctctcc cgcccgtcag cataccacac ccggcgggcg
     3061 cccgcaaggc gggggtagcc cggcggctca agccgccggc taccattacc aaggccgcct
     3121 gcgcggcct
//
LOCUS       DASUYC010001074         2617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_2031034, whole genome shotgun sequence.
ACCESSION   DASUYC010001074 DASUYC010000000
VERSION     DASUYC010001074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2031034"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            436..1221
                     /locus_tag="VF276_17520"
     CDS             436..1221
                     /locus_tag="VF276_17520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX6608702.1"
                     /translation="MLYIGTSGYSYDDWIGTYYPEGIAKTEMLPFYAREFKTTEINYT
                     YYRMPSARTLKAIADKVPDDFVFSIKASKELTHEREDVTFFEQFNTALTPLIEQHKLG
                     CVLAQFPNSFKPSDENRDYLKVFRERMGDLPVAVEFRNSGWITDETMDLLRQYNLGYC
                     CVDEPHLKGLIPPIAVATAPVAYVRFHGRNYQKWWQHDESWERYNYSYSTEELQEWVP
                     KIEHLTQVAPTTYVYTNNHYRGQAVDTVRQIKLMLEQAGEQVA"
     gene            complement(1245..1868)
                     /locus_tag="VF276_17525"
     CDS             complement(1245..1868)
                     /locus_tag="VF276_17525"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX6608703.1"
                     /translation="MATNPGGTQPRYTTRPQMPAPVDITGRVIKLLGKDHIGPDVIRT
                     IAGMGEDVIAVLGAVATGERPASAYERENAVYVLGMLGQDSAVDPLARVLWDPNLNTQ
                     VLAARALGRIGHRGNQEALDLLRRVYNGYWGPSADEPEAARPAADSPGGARVNLRSWA
                     GQTTPTPLPPALAMEIRDILGLNGDHASYVTTPPAAHALESDTGPLP"
     gene            complement(2004..>2617)
                     /locus_tag="VF276_17530"
     CDS             complement(2004..>2617)
                     /locus_tag="VF276_17530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608704.1"
                     /translation="LPLAVTRVFAQGNPRDGTIRDWNEIRDIFDNCNEEPYRADLCVD
                     NIWAPAGIEFRIIVRREIRIADNWAHQLPATVLREFSRAHNLPRLLNVYFLRAVEGAR
                     AWGAADRNPGQNGQQGALWVGDRCFEAPTSECWAEDIVTIAHEAGHFLNLAHRCDNTG
                     AHPRCGPGDEQYLMYGDGTSAASRVLTDEEIFRARQRAWYYRP"
BASE COUNT          461 a          854 c          866 g          436 t
ORIGIN      
        1 gctcctatta gccgtcagcc gtcagccgtc agccatcaga cgtcaaatgc tttgttcact
       61 acgagtatca gcaattccaa caccattgat aggatgccca tcataatggg caacccggcg
      121 ctctccccct tcccgcggcg ggaagggggc cgggggggtt aggttgcacc ccccgcgcgg
      181 atgccgcgtt gccatcttac cacgccgatg ccgggcaccg caaaaggccc ggcgcacgcc
      241 tgtgcccggc tgacgttata tggagagcga agatgccgcc tagaggcgcg tgtgataccg
      301 gttggaccgg cgcgccccca cgccgaattg taggggccgg tttcaaaccg gcccggctta
      361 cccgtcaggc gctgtagggg ccggtttcaa accggcccgg tgcccacgac gaccgaaaac
      421 agacgaggtg caacgatgct ctatatcgga accagcgggt atagctatga tgactggatc
      481 ggaacctatt accccgaagg catcgccaaa accgagatgc tgccgttcta tgcccgcgaa
      541 ttcaaaacca ccgagatcaa ctatacctac taccgcatgc ccagcgcccg cacgctcaag
      601 gccatagccg ataaggtgcc cgacgacttc gtgttcagca tcaaggccag caaggaactg
      661 acccacgagc gcgaggacgt gaccttcttc gagcagttca acaccgccct cacgccgctg
      721 atcgagcagc acaaactggg ctgcgtgctg gcccagttcc ccaactcgtt caagccgagc
      781 gacgagaacc gcgactacct gaaggtattc cgcgagcgga tgggcgacct gcccgtggct
      841 gtcgagtttc gcaactccgg ctggatcacc gacgagacga tggacctgct gcggcagtac
      901 aacctgggct actgctgcgt cgacgagccg catctcaagg ggttaattcc gccgattgcc
      961 gtggcgacgg cgcccgtggc ctacgtacgc ttccatgggc ggaactacca gaagtggtgg
     1021 cagcacgacg agtcctggga gcgctacaac tacagctatt cgaccgagga gttgcaggag
     1081 tgggtgccca agatcgagca tctgacccag gtggcgccga cgacctatgt ctacaccaac
     1141 aaccactatc gggggcaggc ggtggatacg gtgcgccaga tcaagctgat gctggagcag
     1201 gccggggaac aggtggcctg atcgcgggcc ggccgccctg ccgctcaggg cagggggccg
     1261 gtatcgcttt ccagggcgtg cgcggccggc ggcgtggtga cgtaggaggc gtggtcgccg
     1321 ttcaggccga gaatgtcgcg gatctccatc gccagagcgg ggggcagcgg cgtgggggtg
     1381 gtctggccgg cccaggagcg caggttgacc cgcgccccgc ccggactgtc ggcggcgggc
     1441 cgcgccgcct cgggctcgtc ggcgctgggc ccccagtagc cgttgtacac ccggcgcagc
     1501 aggtccaggg cctcctggtt gccgcggtgg ccgatgcgcc ccagggcgcg cgccgccagc
     1561 acctgcgtgt tcaggttggg gtcccacagc actcgcgcca gcgggtcgac ggcgctgtcc
     1621 tgcccgagca tgccgagcac atagacggcg ttctcgcgct cataggcgct cgccgggcgc
     1681 tcgcccgtgg cgacggcgcc caacactgcg atgacatcct cacccatccc ggcaatggtg
     1741 cggatcacgt cgggtccaat atgatctttg cccagtaact taatcacacg cccggtgata
     1801 tccaccgggg ccggcatctg gggccgggtc gtgtagcggg gctgcgtccc gccgggattg
     1861 gtagccatgt ccaacgtcct ttcactgacc gaaaaccatc aaaccgcgga tggtctcctt
     1921 tcattatacc actgtcctgt ctacgcaaga tagcggtcgt tggcatccat catagcacag
     1981 tcccggcggc gcgggctaga gacttaaggc cgatagtacc aggcgcgctg gcgggcgcgg
     2041 aagatctcct cgtcggtgag cacgcgcgag gcggcgctgg tgccgtcgcc atacatgagg
     2101 tactgctcgt cgccggggcc gcagcggggg tgcgcgccgg tgttgtcaca gcggtgggcc
     2161 aagttcagga agtggcccgc ctcgtgggcg atggtcacga tatcctcggc ccagcactcg
     2221 ctggtcggcg cctcgaagca gcggtcgccc acccacagcg ccccctgctg gccgttctgg
     2281 ccggggttgc gatcggcggc gccccaggcg cgggcccctt cgacggcacg caagaagtag
     2341 acgttcagca ggcgcggcag gttatgcgcg cggctgaact cgcgtagcac cgtcgccggg
     2401 agctggtgcg cccagttatc ggcgatgcgg atctcgcggc gcacgatgat gcggaactcg
     2461 atccccgccg gggcccagat attgtccaca cacaggtcgg cccggtaggg ttcttcgttg
     2521 cagttgtcga agatgtcgcg gatctcgttc cagtcgcgga tggtcccgtc gcgcgggttg
     2581 ccctgcgcga agacgcgggt gacggccagc ggcagcg
//
LOCUS       DASUYC010001075         2056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1108426, whole genome shotgun sequence.
ACCESSION   DASUYC010001075 DASUYC010000000
VERSION     DASUYC010001075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108426"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..1989
                     /locus_tag="VF276_17535"
     CDS             130..1989
                     /locus_tag="VF276_17535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608705.1"
                     /translation="MADRDETPERARIEELDPAPPAGPARHWELVLGLLLLLGLAGFG
                     GWQWWQGSRQASAYRAGAQAAAARDWDAAYQAFTQATDYEDAAARAKDAAAQRTERDT
                     QYQRATAARAAGRPLDALAAIRRVLAVAPAYRDSADLAATLQAEVATAALSGTIALRS
                     QATPPGLYAYGAQGWLALAGSDTASQVFDVCPDGAVLFDVPGGDTVPVPVPTLDPDSE
                     RFTSRLRGRRLVVAVPGAPPRATLTLDPSQFQSFGCHELGVWGASYAAGAAQIAGAYG
                     VLQAEHAAYQPFDDPVPHVPVLPGPAWYVLGIAPDGRHIALIDTAGLVPGNWRARIYL
                     ANPDGGGRQLIFDGPGVPGYTPFSADGRYLLLDLQQAADAAGRAHHTVQVVDVTGAAP
                     PRVLADAPQDDGTAILGPRVLARFVQRWGHDPEVLLRTPEADGQTIRLFNVAHPERPP
                     VRYTTDTDLGLLLFWDTSGTAGGLALAWQTWLPDITPRTGTVLYIGDDDRVARTEIAL
                     TGNTQIIRATVRAARLLVATMPYGRGPDDSRWEETFSSRPVGPGAGAPVPHLYTGTES
                     MQPLGRSWYLGPGLLAYTTPSGEVYARAYDGSADVRLDSGVRGFSSVDLTLRR"
BASE COUNT          266 a          774 c          745 g          271 t
ORIGIN      
        1 ggcttgcgca ccggttgcgc gcagacgccg cgcgctgcac gcgacctgct tgacggatcg
       61 cccgccgtgg cgcatattgt tatacacgag ttgccgagcg cggatcgctt gccgggaaag
      121 gagacgccga tggctgaccg ggacgagacg ccggaacggg cgcgcatcga ggaactggac
      181 cccgcgccgc ccgcgggccc ggcgcgccac tgggaactgg tgctgggctt gctgctgctg
      241 ctggggctgg ccggcttcgg cggctggcag tggtggcagg gtagccgcca ggcgagcgcc
      301 taccgggccg gcgcccaggc ggcagcagcc cgcgactggg acgccgccta ccaggccttt
      361 acgcaggcca cggactacga ggacgccgcc gcgcgggcca aagacgcggc ggcccagcgc
      421 accgaacgcg acactcagta ccagcgggcc acggcggcgc gcgcagcggg ccgcccgctg
      481 gacgccctgg ccgccatccg ccgcgtgctg gccgtggcgc ccgcctaccg tgacagcgcc
      541 gatctggccg ccacgttgca ggccgaggtc gccaccgcgg cgctgagcgg caccatcgcc
      601 ctgcgctcgc aggccacccc gcccggcctc tacgcctacg gcgcgcaggg ctggctggcc
      661 ctggcgggca gcgatacggc gagccaggtg ttcgacgtgt gccccgacgg cgccgtgctc
      721 ttcgatgtgc cgggcggcga cacggtgccc gtgcccgtac ccacgctgga cccggattct
      781 gaacgattca cttcccggtt gcgcgggcgg cggctggtcg tcgccgtgcc cggcgcgccg
      841 ccccgcgcga ccctgaccct cgatcccagc cagttccagt cgtttggttg ccatgagctg
      901 ggcgtgtggg gcgccagtta cgcggccggc gcggcgcaga tcgccggcgc ctacggcgtg
      961 ctccaggccg aacatgccgc ctaccagccc ttcgacgacc cggtgcccca cgtgccggtg
     1021 ctgcccggcc cggcctggta tgtgctcggc atcgcgccgg acggccgcca catcgccctc
     1081 atcgacaccg cggggctggt ccccggcaac tggcgggcgc gtatctatct cgccaacccc
     1141 gacggcggcg ggcgccagct catcttcgac ggccccggcg tgcccggcta tacgcccttc
     1201 agcgccgacg ggcgctatct gctgctggac ttgcaacagg ccgccgacgc ggcgggccgg
     1261 gcgcaccata ccgtccaggt ggtggatgtg accggcgccg ccccgccgcg ggtgctggcc
     1321 gatgcgccgc aggacgacgg gaccgccatt ctgggaccgc gcgtgctggc gcgcttcgtc
     1381 cagcgctggg gccacgaccc ggaggttctg ctgcggaccc ccgaggcgga cgggcagacg
     1441 atccgcctgt tcaacgtcgc ccacccggaa cgccccccgg tgcggtacac gaccgacacc
     1501 gacctggggc tgctgctgtt ctgggacacg agcggcacgg cgggcggcct tgcgctggcc
     1561 tggcagacct ggctgccgga tattaccccc cgcaccggca ccgtgctcta tatcggcgac
     1621 gacgaccggg tggcgcggac ggagattgcg ctgaccggaa atacccagat catccgggcg
     1681 actgtgcgcg ccgcgcgcct gctggttgcc accatgccct atgggcgcgg tcccgacgat
     1741 tcccgctggg aagagacctt cagcagccgg ccggtagggc caggggccgg cgccccggtg
     1801 ccccacctct ataccggcac ggagagcatg cagccgctgg gccggagctg gtatctgggg
     1861 ccggggctgc tggcgtacac cacgcccagc ggcgaggtct acgcccgcgc ctacgacggc
     1921 agcgccgacg tgcggctgga ctccggcgta cgcggtttca gctcggtgga cctgaccctc
     1981 cgccgctgag ggccgccccg gcgcgggccg gtttgaaacc ggcccctaca gcgggcggga
     2041 tgcaggcaca ggccgg
//
LOCUS       DASUYC010001076         2164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1432624, whole genome shotgun sequence.
ACCESSION   DASUYC010001076 DASUYC010000000
VERSION     DASUYC010001076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432624"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="VF276_17540"
     CDS             complement(<1..499)
                     /locus_tag="VF276_17540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608706.1"
                     /translation="MQSTQAQRSPAAALAPLRSAGLTGLLQSDGAMLAAMFVAVGVYY
                     LLPGLAFAVIGAILFFALAVLRPYLTPAPIVASMSLFFRPREIGHYLFPLAEFLILAS
                     VAAWVLRDAYELLRRPGGVAALPGIVLDALRRPFVWAAAGFLVAGLLSLVLPHPTHRA
                     EALREF"
     gene            complement(1024..1410)
                     /locus_tag="VF276_17545"
     CDS             complement(1024..1410)
                     /locus_tag="VF276_17545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HEX6608707.1"
                     /translation="MGWRFHKSVKIAPGVRLNLSKRGPGLSFGGRGMRYSVGPSGRRT
                     TVGIPGTGLSYVKQSSTRRGQGCSCCLGLTTLALGVPLAAALTVARARRRAPAPAQPG
                     AARRPAANTAPRPVLYPAAKQTGMPV"
     gene            complement(1433..1573)
                     /locus_tag="VF276_17550"
     CDS             complement(1433..1573)
                     /locus_tag="VF276_17550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608708.1"
                     /translation="MEEILGAVFRIIFRVVAYAVVEIVWELLLQTLFGAIGAGWRRLR
                     GR"
     gene            complement(1589..>2164)
                     /locus_tag="VF276_17555"
     CDS             complement(1589..>2164)
                     /locus_tag="VF276_17555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608709.1"
                     /translation="PLRRLLPSPVQTNWRIHVRDPRTGIQGIYFVTNAINSTLHALPA
                     RMLSEGMPMHVLRRAAIRPTGDGGWQMDLDPGPGTAPDAHAVLRPTAAPPADGPWQVC
                     FPSYLAMLAYCVPQDRAMSSQPWYARITRQEIHLGIPLAACEPLAGTVVSQAAQAFVG
                     NATPFCFRVAQVAFRFDREEHDPLYSTIIPT"
BASE COUNT          349 a          714 c          808 g          293 t
ORIGIN      
        1 ggaactcacg caacgcctcg gcgcgatggg tggggtgggg cagcaccagc gacagcagcc
       61 cggccaccag gaagcccgcc gccgcccaca cgaagggccg ccgcagcgcg tccagcacga
      121 tgcccggcaa cgcggccacc ccgccgggcc gccgcagtaa ctcgtaggcg tcgcgcagca
      181 cccaggcggc caccgaggcc aggatcagga actcggccag cgggaagagg tagtggccga
      241 tctcgcgcgg gcggaagaag agcgacatgc tggcgacgat gggcgccggg gtcaggtagg
      301 ggcgcagcac ggccagcgcg aagaacagta tcgcgccgat cacggcgaag gccaggcccg
      361 gcagcagata atagacgccc acggctacga acatcgcggc cagcatggcg ccatcgctct
      421 gcaagaggcc ggtcagcccc gcgctacgaa gcggggccag ggccgccgcc ggagaacgct
      481 gcgcttgtgt cgattgcatg gatgaggctc ttcttttccc gttagcgcgc taattgataa
      541 accatcgtcg cgatcacgca caggccggtt tgaaaccggc ccctacaccg ccgtcgggcc
      601 acgcgggccg gtttgaaacc ggcccctaca gcggcagccg gacggatccg cccatagcgt
      661 cgccgtttgg catgggccgg tttgaaaccg gcccctacag cgccttcgag cgacgtgggc
      721 cggtttgcaa ccggccccta caccggcagt cgagccgttc ggcctacagc gccttcgggc
      781 gacgcgggcc ggtttgaaac cggcccctac atgggctggt cgcgccgccg ggccggcaaa
      841 taatgccccg gtcgcggtgt aggggccggt ttgaaaccgg cccgcgtggg tcacagcgcc
      901 gattccctga cggcaggccg gggaggtagg gcggcaggcg ggtttgaaac ccgcccctac
      961 accggcatcc cggctggcgg caggccgggg ggatggggcg gcaggcgggt ttgaaacccg
     1021 cctctacacc ggcatcccgg tctgtttagc agcggggtac agtacaggtc gcggcgctgt
     1081 attggcggcg gggcggcggg ccgcgccggg ttgggccggc gcaggggcgc ggcggcgagc
     1141 ccgggcgacg gtcagggcgg cggctagagg cacgcccagg gcgagcgtgg tgaggccgag
     1201 gcagcagcta cagccttgcc cgcgccgcgt gctgctctgc ttgacgtagg acagcccggt
     1261 gccggggatg ccgacggtgg tgcgccggcc gctgggcccg acgctgtagc gcatcccgcg
     1321 cccgccgaag ctcagccccg gtccccgctt gctcaggttg agccgcaccc ctggcgcgat
     1381 cttgacgctc ttgtgaaatc gccagcccat gtttgttcct ccgtatagcc gcctaccgcc
     1441 cgcgtaaccg gcgccacccg gcgccgatgg cgccgaacag cgtttgcagc agcaactccc
     1501 acacgatctc cacgacggcg taggcaacga ccctgaagat gatccgaaag acggcgccga
     1561 gtatttcttc cattaatgta gcgcactcct acgtggggat tatggttgag tatagcggat
     1621 cgtgttcctc gcggtcaaag cggaaggcga cctgggccac gcggaagcag aacggcgtgg
     1681 cattccccac gaacgcctgc gccgcctggg agacgacggt gccggccagc ggttcgcacg
     1741 cggccagcgg gatgcccagg tggatctcct ggcgggtgat gcgcgcgtac cagggctggc
     1801 tggacatggc gcggtcctgc ggcacgcaat acgccagcat cgccagatag ctggggaagc
     1861 agacctgcca ggggccgtcc gcgggcgggg cggcggtcgg gcgcaggacg gcgtgagcat
     1921 cgggcgcggt gccggggccg gggtcgaggt ccatctgcca gccgccgtcc ccggtggggc
     1981 ggatggccgc gcggcggagg acgtgcatcg gcatgccctc ggagagcatg cgggccggga
     2041 gggcgtgcag cgtgctgttg atggcgttgg tcacgaagta gatgccctgg atgccggtgc
     2101 gcgggtcgcg gacgtggatg cgccagttgg tctggaccgg cgacggtagc agccggcgca
     2161 gcgg
//
LOCUS       DASUYC010001077         2942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_155134,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001077 DASUYC010000000
VERSION     DASUYC010001077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155134"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="VF276_17560"
     CDS             <1..503
                     /locus_tag="VF276_17560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608710.1"
                     /translation="AAAERAQPTAATEAAAETPVPADVGARAAALIRDQAEVLVMRIM
                     YDTQELVRVGAHGVDPVTARAIVETYAAALEHGDARTFAYTYSRAARPMVPQINDQVL
                     PFRLGLQIAATIEGILRDTFQQGFAGDPALQAASGQLLAQLSAPAQQALLIEPREIIQ
                     WQAPRI"
     gene            complement(637..1992)
                     /locus_tag="VF276_17565"
     CDS             complement(637..1992)
                     /locus_tag="VF276_17565"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6608711.1"
                     /translation="MDELLPGNPELATLIGQRLRDVRRARGWSQQALAEGLFSKGYVS
                     SIEHGKIFPSVRALRALAGRLGVDMSEFFDPALLGGMEDAEAAEEAEEAQRRRLLLLE
                     VRALAAAEQPGVSSRLRQLEAERLSPAEQVDLHLAAGQAALAVGDADAALAAFEQALA
                     TLPAATGDESDGARALPVHLALGEALLARGQATLAVEHFAQVQQLVQESAAPDPALRR
                     AAVFGLARAYAAAGEVPRARAAFQEALTLAGESATLELLVGQLLGLSRQAGAAGQWDA
                     ATRYGEQALLLAQTVQTLRAVVQTHLHAGALLRALGERGARDLIEQATRLAQQSGTEA
                     VIAEVEAELAALEIEAGDLPAAEAALARATQAADRAADRRMQARVQFLRARLDQAAGD
                     LAAAETGLRTVLDGLTEPGDRPLRADAAFHLAQVLVAGGRHVAAAPYYEEAFRARTAA
                     E"
     gene            2244..2873
                     /locus_tag="VF276_17570"
     CDS             2244..2873
                     /locus_tag="VF276_17570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608712.1"
                     /translation="MAQLPEADFNSNDRAHFAFLDDDAPGDGAGPPADVFAPPVRVAG
                     PVVPLPIPVNPANGWQALDADYRVLPEPPTALSLAALLRRDAPPVAVYQRARLEPPIL
                     LGWVAVSCLWLGALPWLVLGLGMAGFSLFMRRTFAMCGMEGHAGVYRALGAGQMLLGV
                     VAITGPGPFAPVLGLYAAGVLLAWVFVECRYMVRVERQNLREQPAPLIF"
BASE COUNT          379 a         1041 c         1100 g          422 t
ORIGIN      
        1 ccgccgccgc cgagcgcgcg cagcccaccg ccgccaccga ggcagcggcg gagaccccgg
       61 tgcccgcgga cgtgggcgcg cgcgccgcgg ccctgatccg cgaccaggcc gaagtgctcg
      121 tcatgcgcat catgtacgat acccaggaac tggtgcgcgt gggcgcgcac ggcgtcgatc
      181 cggtcacggc gcgggccatc gtggagacct acgccgccgc cctggagcac ggcgacgcgc
      241 gcaccttcgc ctacacctac agccgcgccg cccgcccgat ggtgccgcag atcaacgacc
      301 aggtcttgcc cttccggctg ggcctgcaaa tcgccgcgac catcgagggc atcctgcgcg
      361 acaccttcca gcagggcttc gcgggcgacc cggccttgca ggcggccagc gggcagttgc
      421 tggcccaact ctcggccccg gcccagcagg ccctgctgat cgagccgcgc gagatcatcc
      481 agtggcaggc gccgcgcatc tagccgcgcg agcatagcgg agccgggccg ggttcaaacc
      541 ggcccctaca ccgccaatag ctggcacaag caggcccccg gtggtactcg ccaccggggg
      601 cctttttgcc gtggagggcg ccgtgccgtg cggggactac tccgcggcgg tgcgggcgcg
      661 gaaggcttct tcgtagtagg gggcggcggc gacgtggcgg cccccggcga cgagcacctg
      721 ggccaggtgg aaggcggcgt cggcgcgcag cgggcggtcg cccggctcgg tgaggccgtc
      781 gagcacggtg cgcaggccgg tctcggcggc ggcgaggtcg cccgcggcct ggtcgagccg
      841 ggcgcgcagg aactgcacgc gcgcctgcat gcgccggtcg gcggcgcggt cggcggcctg
      901 cgtggcgcgg gccagggcgg cttcggcggc gggcaagtcg ccggcttcga tctccagcgc
      961 ggccagctcg gcttcgacct cggcgatgac ggcttcggtg ccgctctgct gggccaggcg
     1021 cgtggcctgc tcgatcaggt cacgcgcgcc ccgctcgccg agcgcgcgga gcagggctcc
     1081 ggcgtggaga tgggtctgca cgacggcgcg cagggtctgg acggtctggg ccagcaacag
     1141 cgcctgctcg ccgtagcggg tggcggcgtc ccactgcccg gcggcgccgg cctggcggct
     1201 cagcccgagc agttgcccga ccagcaactc cagcgtggcg ctctcgccgg ccagcgtcag
     1261 ggcttcctgg aaggcggcac gggcccgcgg cacttccccg gcggcggcgt aggcgcgggc
     1321 cagcccaaac accgcggcgc ggcgcagggc agggtcgggc gcggcgcttt cctgcaccag
     1381 ttgctgcacc tgggcgaaat gctcgacggc gagcgtagcc tggccgcgcg ccagcagcgc
     1441 ctcgcccagc gccaggtgca ccggcagggc gcgggcgccg tcgctctcgt cgccggtagc
     1501 ggcgggcagg gttgccagcg cctgctcgaa ggcggccagg gcggcgtcgg cgtcgcccac
     1561 agccagggcg gcctgcccgg cggccaggtg caggtccacc tgctcggcgg gactcaggcg
     1621 ctcggcctcc aactggcgca ggcgggagct cacgccgggc tgctcagccg cggccagggc
     1681 gcgcacttcc agcagcagca ggcgccggcg ctgggcctcc tcggcctcct cggcggcttc
     1741 ggcgtcttcc atgcccccca gcagcgcggg gtcgaagaac tcggacatat cgacgccgag
     1801 ccgcccggcc agcgcgcgca gggcgcgcac cgaggggaag atcttgccat gttcgatgct
     1861 gctgacgtag cccttggaaa ataggccctc ggcgagggcc tgctggctcc agccgcgcgc
     1921 ccgccgcaca tcgcgcaggc gctggccgat taacgtggcg agttcagggt tgccgggtag
     1981 tagttcatcc acagtctggt cctccgccgc tgcgtggttc agtggcaccc aacttgggtt
     2041 tactgttaat gagaataggc cggataggac gcattatacc aaccttgccg cctggtgaca
     2101 agggccaacc agcacctgtc gtcgatactc cccgcagaga cgacaggtat tccagcgact
     2161 ggggatttgg gctataatga gatccggtgg ggtgtggcat ccgggggccg acggccgccg
     2221 gggagccgag gggaggtagc gcgatggcgc agttgccgga agcggacttt aactccaacg
     2281 accgggccca tttcgcgttt ctcgacgacg acgcgccggg cgacggggcc gggccgccgg
     2341 cagacgtgtt cgcgccgccc gtgcgcgtgg ccgggccagt cgtaccgctg cccatcccgg
     2401 tgaaccccgc gaacggctgg caggcgctcg acgccgatta tcgcgtgctg ccggagccgc
     2461 cgacggcgct atccctggcc gcattattgc gccgcgacgc gccgcccgtg gccgtatacc
     2521 agcgcgcccg gctggagccg ccgatcctcc tgggctgggt ggccgtgtcg tgtttatggc
     2581 tgggcgcgct gccctggctg gtcctcggcc tggggatggc cggcttctcc ctgttcatgc
     2641 ggcgcacgtt cgccatgtgc ggcatggagg gccacgccgg ggtctaccgg gcgttgggcg
     2701 ccggccagat gctgctgggc gtggtggcta tcaccgggcc ggggccgttc gcgccggtgc
     2761 tcggcctcta tgccgcaggc gtgctgctgg cgtgggtgtt cgtcgagtgc cgctatatgg
     2821 tccgcgtcga gcggcagaac ctgcgcgagc agcccgcgcc gctgatcttc tgagcagtct
     2881 gccatacatg aattgtcact ctgagcgcag cgaagagtct cgtcgtttgg gctacgagac
     2941 gg
//
LOCUS       DASUYC010001078         4890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1346928, whole genome shotgun sequence.
ACCESSION   DASUYC010001078 DASUYC010000000
VERSION     DASUYC010001078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4890
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346928"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VF276_17575"
     CDS             complement(<1..441)
                     /locus_tag="VF276_17575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608713.1"
                     /translation="MRHRSWSPGLFALIVCLALAAFALGASQVGAHNAAGPVLGTAPA
                     PTGGHPAAPGTYVGDYTVVTSTGTLVPGATDIGNHGDDAVTGITLPFPVTFYDRTFVT
                     ATVSSNGNLQFVSSNSEWQNGPLPDGALNYAMLPHWDDLTTDNTG"
     gene            complement(725..2392)
                     /locus_tag="VF276_17580"
     CDS             complement(725..2392)
                     /locus_tag="VF276_17580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608714.1"
                     /translation="MSIRPITGTGRRGRIAALAAAAALLLIALLDGLICARAGLGRTG
                     SWSFPIDDAYIYANYVRGAEAGGLFQYNPGEQSGGVTGAGWMLLLLVAHPLTGLLGDA
                     PAGLAPTAAQSVMPATALTAGRLYLAAYGWGALLLAGAALACGWLAWEITRALPPPAA
                     WTAALLAGGALLADQNVVWGAYSGLELPLSLLLIALAPATLLYELRVHGLTRSQPLRW
                     SLPAVALLPWARPELAVVGAAGVLWLAWGAARGRGSWRTVAAYGAAVLAGLAALAAFF
                     WWGTGYPLPSSFYAKVAGARLDTLTAALQEWVLAGAWQPFALSSAAALGTGLLYWAGR
                     RAAKAPSRTPAPAGLPALCALAYFAAMLITLRWFGQEDRYILPIHALALPLAAGGIAV
                     LAARLPAFRTPVAGRTWVAGAAVVALLALGVHIATLRVWASSDYALFIQNIEDAHVAP
                     ALWLAANAAPGAVVAAEPIGAVRLFSGHPTVDIVGLTTPAQLGHYQDWPATRALLRAR
                     NAQYLLFYPRWWPTPNQPLPWAHEAQRFLVPDNRIAGDSPIAVYRLD"
     gene            complement(2389..>4890)
                     /locus_tag="VF276_17585"
     CDS             complement(2389..>4890)
                     /locus_tag="VF276_17585"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX6608715.1"
                     /translation="AALSIPLSLHLRWLLALIALSTYITGTDSPLPAVLAGLILVFNT
                     TGYLLRRAGIPLASPLTAVADLVFISMLISQRGGALSETYLLYTLPIFYLGLQYGWRG
                     AIAACTGTLFAYAGTIAATMQVVVLPLGFDPASHFLVRLLYFVFVTAVVSVIVREQTH
                     NQHTVAQADGRIREQVRRLEAIQRITEKLNTAPASADVARVIAEETRGIIPFKNCRVH
                     VVESGPDGVWLPLVAFYGQLANDRPLPAGALRVRPGEGITGWVAVHGEPLLIHDAMHD
                     PRARHIPGTPYTPNSLLAVPVLRGKQLKGVIVLGQAGLHAFCDDDLKLMMTLANAAAI
                     ALTNIESRESLARQARTDAVTGLPHHGAFQAALVEAMEEATRRDETLALALLDIDGFR
                     SYNERMGLAAADRALRHVGMTLEHICHTASVSQLDSPGEEGDAGCRAPLVQCFRIGGD
                     EFALLLSGPKGCRDAAIDVVRECIRTVSAGQPDPVQRVTVSAGMGFFPVDATSRADLL
                     DTAEAALYLVRQTGGNRLGLADAEARETLHLRHTLEQMVQASLAESGSAEAVQHLIAE
                     AAGVSRSSRHATLAEQLTTEALRALAAAIDAKDDYTRGHSERVAATATEIARRMGMPL
                     TEVEHVTTAARMHDIGKIGVPDHILHKGSALTAEEQAVVATHPDVGARILAPIHTLRE
                     VVPIVRHHHEHYDGNGYPLGLCAEDIPLGARVVAVADAIDAMITDRPYRRGMPITVAL
                     TELQRWAGSHYDPRVVATAVQLYGAGGTGLALRGVLPAARATRPSDDEVAAAMTAPLS
                     LVLHPEAVGTPAEFALRAIVRGDAETRPIHADIPH"
BASE COUNT          782 a         1635 c         1781 g          692 t
ORIGIN      
        1 gccggtgttg tcggtggtca ggtcgtccca atgcggcagc atggcataat tgagcgcgcc
       61 gtcgggcagc gggccgttct gccactcgct gttgctgctc acaaactgga gattgccgtt
      121 gctgctgacg gtggcggtaa cgaatgtccg gtcatagaat gtgaccggaa agggcagggt
      181 gatgcctgtg acggcatcgt cgccgtggtt gccgatgtcg gtcgcgcccg gcacgagggt
      241 gcccgtggag gtgacgaccg tgtagtcgcc gacgtaggtg ccgggcgccg ccgggtggcc
      301 gccggtcggg gcgggggccg tgccgagcac cgggccggcg gcattatgcg ccccgacctg
      361 ggatgcgccc agggcgaacg cggccagcgc caggcacacg ataagggcga atagaccagg
      421 ggaccaagaa cgatgcctca tagcaggatg gacgttcctt tccgagggcg cctcgcgcca
      481 ctcactacat gaaaacccac cacgcgccgc ccggcccaag accaacacgc cttgtcgcgg
      541 gcatcagata tccggcgcag cggtggtgag cgtgtaggcc gcgcagttat acgatgaata
      601 ggagagggat aattgcctgt agcccagtaa ccagtatagg ggatgccgcc gggtttgcca
      661 ggggtacaag gggcggaaag ccggggctcc ctggtcctta ctatgctggc ccgatggtcc
      721 ctggtcagtc gaggcgatac acggcgatgg ggctgtcgcc cgcgatgcgg ttatcgggaa
      781 cgaggaagcg ctgcgcctcg tgcgcccagg gcaggggttg gttgggggtg ggccaccagc
      841 gcgggtagaa gagcaggtat tgcgcgttcc gcgcccgcag tagcgcacgg gtggcgggcc
      901 agtcctggta gtggcccagt tgggcgggcg tggtcaggcc gacgatgtcg accgtgggat
      961 ggccgctgaa caggcgcacc gcgccgatcg gttcggcggc gaccacggcg cccggcgccg
     1021 cgttggcggc cagccagagc gcgggcgcga catgcgcgtc ctcgatgttc tggataaaca
     1081 gcgcatagtc gctcgacgcc cagacgcgca gcgtagcgat atgcaccccc agcgccagca
     1141 gcgcgacgac ggcggccccg gccacccacg tccgcccggc gacgggtgtg cggaaagccg
     1201 gtagccgagc ggcgagcacg gcgatcccgc ccgcggcaag gggcagggcc agcgcatgga
     1261 tcggcaagat gtagcgatcc tcttgcccaa accagcgcag cgtgatcagc atggccgcaa
     1321 agtaggccag ggcgcacagc gccggcaggc ccgccggggc cggagtgcgg gatggggcct
     1381 tggcggcgcg acggcccgcc cagtagagca ggcccgtgcc cagcgccgct gcgctgctca
     1441 gcgcgaacgg ctgccacgcg ccggccagca cccactcctg cagcgcggcg gtcagcgtat
     1501 ccaggcgtgc gcccgcgacc ttcgcgtaaa agctgctcgg cagtgggtag ccggtgcccc
     1561 accagaagaa ggcggccagc gcggccagtc cagccagcac ggcggccccg taggccgcta
     1621 cggtacgcca actcccgcgt ccgcgggcgg cgccccaggc cagccagagc acccccgctg
     1681 cgcccacgac cgccagttcg ggccgcgccc agggcagcag ggcgaccgcg ggcaggctcc
     1741 agcgtagcgg ctgggaccgg gttagaccgt ggacgcgcaa ctcgtacagg agggtcgccg
     1801 gggccagcgc gatcagcagc agcgataggg gcagttccag cccggagtag gcgccccaga
     1861 ccacgttctg gtcggccagc agggcgcctc cggcgaggag ggccgccgtc caggcggcgg
     1921 gcggcggcag ggcgcgcgtg atctcccagg ccagccagcc gcaggccagc gccgcgcccg
     1981 ccagcagcag ggcgccccag ccataggcgg ccaggtagag ccgcccggcg gtgagcgccg
     2041 tcgcgggcat gaccgactgg gctgccgtgg gcgcgaggcc cgcaggcgca tcccccagca
     2101 ggccggtcag cgggtgcgcg accagcagca gcaacatcca gcccgcgccg gtgacgccgc
     2161 cgctctgttc gcccggatta tactggaaca ggccgccggc ttcggcgccg cgcacatagt
     2221 tggcgtagat ataggcgtcg tcgataggga acgaccagga cccggtgcgg cccagcccgg
     2281 cgcgggcgca gatcaggccg tcgagcaggg caatcagcag cagcgccgcc gccgccgcta
     2341 gcgcggcgat gcggccccgg cgcccggttc cggtgatggg gcggatgctc aatgcggaat
     2401 atcggcgtgg atcgggcggg tttcagcgtc gccgcgcaca atggcgcgca gggcaaattc
     2461 ggcgggtgtg cccaccgcct cggggtgcag caccaggctc agcggtgcgg tcatagccgc
     2521 agcgacctcg tcgtcgcttg gacgggtggc gcgcgcggcg ggcagcacac cgcgcagggc
     2581 cagcccggtg ccgcccgcgc cgtaaagttg cacagcggtg gcgaccaccc gcggatcata
     2641 atggctgccc gcccagcgtt gtagttcggt cagcgccaca gtgatcggca tcccgcgccg
     2701 gtaggggcga tcggtgatca tggcgtcaat ggcgtcggcc acggcgacca cccgcgcgcc
     2761 cagcgggatg tcctcagcgc agagcccgag cggatagccg ttgccgtcgt agtgctcgtg
     2821 gtggtggcgc acgatgggca ccacttcgcg cagcgtatgg atcggcgcga gaatccgggc
     2881 gccgacatcc gggtgggtgg cgacaaccgc ctgttcctcg gcggtgagcg cgctgccctt
     2941 gtgcagaatg tgatccggca caccgatctt gccgatatcg tgcatgcgcg ccgccgtggt
     3001 gacgtgttcg acttcggtca gcggcatgcc catgcggcgg gcgatctcgg tcgccgtcgc
     3061 cgccacgcgc tcgctgtggc cgcgtgtata atcgtccttc gcgtcgatgg cggcggccag
     3121 ggcccgcaac gcctcggtcg tcagttgctc ggccagggtg gcatggcggc tgctgcggct
     3181 caccccggcg gcctcggcga tcaggtgctg caccgcctcc gcggacccgc tctcggccag
     3241 gctggcctgg accatctgtt ccagcgtatg gcgcaggtgc agcgtctcac gcgcctcggc
     3301 gtcggccagg cccaggcggt tgccacccgt ctggcgcacc aggtagaggg ccgcctcggc
     3361 ggtgtccagc aagtcggcgc gcgaggtggc atccaccggg aagaacccca tgcccgccga
     3421 taccgtgacg cgctgcaccg ggtccggctg cccggcgctc acggtacgga tgcactcgcg
     3481 caccacatcg atggccgcgt cgcggcagcc cttgggtccg ctcagcagca gcgcgaattc
     3541 gtcgccgccg atgcggaagc actggaccag cggcgcccgg cacccggcat caccttcttc
     3601 ccctgggcta tccagctgcg acacactcgc ggtgtggcag atatgttcca gggtcatgcc
     3661 cacgtggcgt agcgcccggt cggcggcggc caggcccatg cgttcgttgt agctgcggaa
     3721 cccgtcgatg tcgagcaggg ccagggcgag ggtctcgtcg cgccgcgtgg cttcctccat
     3781 ggcctcgacc agggcggcct ggaaggcgcc gtgatggggc agacccgtta ccgcatcggt
     3841 gcgtgcctga cgggccagcg attcgcggct ctcgatattg gtcagcgcaa tcgccgccgc
     3901 gtttgccagg gtcatcatca gcttcaggtc gtcgtcgcag aaggcgtgca ggccggcctg
     3961 gcccagcacg ataacgccct tcaactgttt gccccgcagc acgggcaccg ccaacagcga
     4021 gttgggggtg tagggtgtgc cggggatgtg gcgggcgcgc ggatcgtgca tggcatcgtg
     4081 gatcaagagc ggttcgccgt gcacggccac ccagccggtg atgccctcgc cggggcgcac
     4141 tcgcagcgcc cccgccggca agggcctgtc gttcgcgagt tgcccgtaga aggccaccag
     4201 cggcaaccag acgccgtccg gtcccgactc gaccacatgc acacggcagt tcttgaaggg
     4261 aatgatgccg cgggtttcct cggcaatcac gcgcgctaca tcggcgctcg ccggcgcggt
     4321 gttgagtttc tcggtgatgc gctggatcgc ttccaggcgg cgcacctgct cgcgtatgcg
     4381 cccatcggct tgtgcgaccg tatgctggtt gtgagtctgt tcgcgcacga tcacgctgac
     4441 caccgccgtg acgaagacga aatagagcag gcgcaccaga aaatgcgagg ccgggtcaaa
     4501 ccccagcggc aacaccacga cctgcatggt ggccgcaatc gttcccgcgt aggcgaacaa
     4561 cgtgcctgtg caggccgcga tcgcgccgcg ccagccgtat tgcagcccga ggtaaaagat
     4621 cggtagcgtg tagagcaggt acgtctcgct aagcgcgccg ccgcgctggc tgatcagcat
     4681 cgagataaac accaggtcgg cgacggcggt cagcggggaa gccaggggaa tcccggcgcg
     4741 ccgcagcagg tagccggtcg tattgaagac cagaatcagc ccggccagga cggcgggtag
     4801 gggggaatct gttcccgtga tataggtgga cagcgcgatc agcgccagga gccagcgcag
     4861 atgcaagctg agggggatcg ataatgcagc
//
LOCUS       DASUYC010001079         4428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_415847,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001079 DASUYC010000000
VERSION     DASUYC010001079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415847"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VF276_17590"
     CDS             <1..568
                     /locus_tag="VF276_17590"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX6608716.1"
                     /translation="SAAFSPDGKLVATGDNAGAVRLWDAATGQEVRRFIGHTAGYVRV
                     SFSRDGKQIVTAGDDTTARVWDVQTARELQRFTVPDAGMKAAEFSSDGRLVLTAGDDG
                     VARLWDVATGQEVRRFTGHSDQVKAAVFSPDGKYILTASLDKTARRWQIDYHDTIRSL
                     CAELTRDLTPEERSQYGIADQEPTCSAP"
     gene            629..1111
                     /locus_tag="VF276_17595"
     CDS             629..1111
                     /locus_tag="VF276_17595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004436463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HEX6608717.1"
                     /translation="MDTQSMHGQGSAPGDEAAVRGLYAALLEQWNRRDAAGMAALFAE
                     AGHLVGYDGSVVNGRAAIAGHLAPIFADHPTPAYVGKVREVRFLTPDVAVLRAVSGLV
                     PAGTRDLNPALNAVQSLVAARHDGQWRVELYQNTPAAFHGRPEESAALTAELRALLTE
                     "
     gene            complement(1203..>4428)
                     /locus_tag="VF276_17600"
     CDS             complement(1203..>4428)
                     /locus_tag="VF276_17600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608718.1"
                     /translation="ADARVAGSVFGHNVVVEAGARVDGDIYSLGDLTLGAGAEVGGMA
                     LAGDTLVAGGASRIQGSGGAQGGTVRLGPGARLTGWATAAEGLTADSAAAGGLLAGAD
                     LALNGAVEIGATAAGSAAGAVRVDAPVRIGGTTAGSESVHAWHSDGPDATAEPPGQAL
                     SSVLTRRGRGLAEDALRAAETPATLTAPQPVDTASEDGLPVTPATEGPTLYLPATLAR
                     ADDDPPPGLPAEPEAWEGPLAGAWMLAQVWREFLGGLRLPAIIAVLFTLLCWGAAVAL
                     LAGGQGGAAGLVLLIWAGALLVAAGLLWMFRPRPLELVRLWWAWETWPVAGGVLLWDT
                     LDAGREPGYLEPAAPLPPLADLAAAVAAADLPDTAVQERLAALGTALETLAWKSWPGV
                     FAPKPPDAPPAAEAPPSVVDELLQLPVDVLPAEQLPGMLVLAPRLDPEQVRAGADRLA
                     AWPTYQAAGREVWHTLNRRLDSLDDVLATWRGEAATAAERAATATATLDAVRARAVDL
                     ADETFAALETEIGPDLDRVGLLHVEGGKLISACYERIRDDLQATLAREQDRLRAQIAT
                     TERKLQQQETVLSEIDAELQPLTDRWQALAALHERTGAALRQDWKQARAQTARAVAEG
                     DLYVSPRPIAVEQAPTVVAQAAQDLTKLARQAEDLEALCAKDEQDLPVQLPWPAWPAQ
                     ITAPLAVDSEVAALSALLRRARNEPVRARGRLDTLNSLRARVTHALERALGAQETLAG
                     ALAAEHAPAGADPGRLAGAKSQADGLTQRLYALAGTLARLQRRLEVATVAYDEVADNL
                     AYTLKGGRGGTTWDQARRLQDDYVRLQGERTAALNAVSATGAALATAHADAQRLQTTT
                     ETEQQRLSRAEKDLIAWSEEYAGREEATLRRHVELLRARHAAVLAGFAPRRGWLDALA
                     AFDAAAAQLKVDGTDRLRERVRLLAALADGRIAETAAARLDALWPADSWPATDMLYLV
                     PLWLLRYRPLGARSAPRLCAVTPGTVRAAPPRRFALTGFMVDPQPGLAARFAADVLTW
                     ANRPPGVTTPLEGANLLAPGAHLAARWQALAAHGLVPPWLGRLL"
BASE COUNT          555 a         1592 c         1722 g          559 t
ORIGIN      
        1 cagcgcggcc ttcagccccg acggcaagct ggtcgcgacc ggcgacaacg ccggcgcggt
       61 gcggctgtgg gacgcggcga ccgggcagga agtgcgccgc ttcatcggcc atacggcggg
      121 gtatgtgcgg gtcagctttt cgcgggacgg caagcagatc gtcactgccg gcgacgatac
      181 gaccgcgcgc gtgtgggatg tgcagaccgc gcgggaacta caacgcttta cggtccctga
      241 tgccggtatg aaggcggcgg agttctcgtc ggatggacgg ctggtcctca cggcgggcga
      301 tgatggcgtg gcgcggctgt gggacgtggc gaccgggcag gaagtccgcc ggttcaccgg
      361 ccacagcgac caggtgaagg ccgcggtctt ctcgccggac ggcaagtaca tcttgacggc
      421 cagcctggat aagacggccc ggcgctggca aatcgactac catgacacga ttcgctcgct
      481 ctgtgccgaa ttgacccgcg acctgacgcc ggaagagcgg tcgcaatacg gcatcgctga
      541 ccaggagccg acgtgctcag caccatagca gccctatccc ggccccacaa ttcgtcttca
      601 cgggccggat agccagggag aatgtggcat ggacacccaa tcgatgcacg gacaaggatc
      661 agcgccgggc gacgaagccg cggtgcgcgg gctgtatgcc gcgttgctgg agcagtggaa
      721 ccggcgcgac gccgcgggga tggcggcgct gttcgccgag gccggccacc tggtcggcta
      781 cgacggcagc gtggtgaacg ggcgggcggc catcgccggg cacctggcgc ccatcttcgc
      841 ggaccacccg acgccggcct acgtgggcaa ggtgcgcgag gtacgcttcc tgacgcccga
      901 cgtggccgtg ctgcgcgccg tctcgggcct ggtgcccgcc ggcacccgcg acctcaatcc
      961 cgccctcaac gccgtgcaga gcctggtcgc cgcccggcac gacggccagt ggcgcgtcga
     1021 actctaccag aacacacccg cggccttcca cggccgcccg gaggaaagcg ctgcgctcac
     1081 ggcggagttg cgggcgttgc taacggaata gattcaccgc gaaggaacga aggtaacgaa
     1141 gatcttctat attaatcgtc ttcgttgcct tcgtttcttc gcggtgaaat tgtgcgaatc
     1201 ggttacagca accggcccag ccagggcggc acgaggccgt gggcggccag ggcttgccag
     1261 cgggcggcca ggtgcgcgcc gggggccagc aggttcgcgc cttcgagcgg ggtggtcaca
     1321 cccggcgggc ggttggccca ggtgagcacg tcggcggcga agcgggcggc caggccgggc
     1381 tgcgggtcta ccatgaagcc ggtcagggcg aagcggcgcg gcggggcggc gcgcaccgtg
     1441 cccggcgtga cggcgcagag gcggggcgcc gagcgcgcgc ccagcgggcg gtagcgcagc
     1501 agccagagcg gcaccaggta gagcatatcg gtggcgggcc aggagtcggc gggccagagg
     1561 gcatccagcc gcgcggcggc ggtctcggcg atgcgcccgt cggccagggc ggccagcagg
     1621 cgcacccgct cgcgcaggcg gtcggtgccg tcgaccttca actgcgcggc ggcggcgtcg
     1681 aaggcggcca gcgcgtcgag ccacccgcgg cgcggggcga acccggccag cacggcggcg
     1741 tggcgagcgc gcagcagttc cacatgacgg cgcagggtcg cttcttcccg cccggcgtac
     1801 tcctcgctcc aggcgatcag gtccttctcg gcgcggctca ggcgctgctg ctcggtctcg
     1861 gtcgtggtct ggaggcgctg cgcgtcggcg tgggcggtcg ccagtgccgc gccggtggcg
     1921 ctgacggcgt tcagcgcggc ggtgcgctcg ccttgcaggc ggacgtagtc gtcttgcagg
     1981 cggcgggcct ggtcccaggt ggtgccgccg cgcccgccct tgagcgtgta ggccaggttg
     2041 tcggccacct cgtcataggc caccgtggcg acctccaggc ggcgttgcag gcgggccagg
     2101 gtgccggcca gggcgtagag ccgctgggtc agcccgtcgg cctggctctt ggcgccggcg
     2161 aggcggcccg gatcggcgcc cgccggggcg tgctcggcgg ccagcgcgcc ggccagcgtt
     2221 tcttgcgcgc ccagcgcgcg ctccagcgcg tgggtgacgc gggcgcgcag gctgttcagc
     2281 gtgtcgaggc ggccacgggc gcgtaccggc tcgttgcgcg cccggcgcag cagcgcgctc
     2341 agggccgcca cctcgctatc gacggccagc ggcgcggtaa tctgcgcggg ccaggcgggc
     2401 cagggcaact gcacgggcaa atcctgctcg tccttggcgc agagcgcttc caggtcctcg
     2461 gcctggcggg ccagcttggt caggtcctgg gcggcctggg cgacgacggt gggcgcctgc
     2521 tccacggcga tgggccgcgg cgacacgtag aggtcgccct cggcgacggc gcgggcggtc
     2581 tgggcgcggg cctgcttcca gtcctggcgc aaggccgcgc cggtgcgctc gtgcagggcg
     2641 gccagggcct gccagcggtc ggtcagcggc tgcaactcgg cgtcgatctc gctcagcacc
     2701 gtctcctgct gctgcaactt gcgctcggtc gtggcgatct gggcgcgcag gcggtcttgc
     2761 tcgcgggcca gcgtggcctg caaatcgtcg cggatgcgct cgtagcaggc gctgatcagc
     2821 ttgccgcctt cgacgtgcaa caggcccacg cggtcgaggt cggggccgat ctcggtctcc
     2881 agcgcggcga aggtctcatc ggccaggtcc acggcgcggg cgcgcacggc gtcgagggtc
     2941 gcggtggccg tggcggcgcg ctcggcggcg gtggcggcct cgccgcgcca ggtcgccagc
     3001 acgtcgtcca ggctgtcgag ccggcggttg agcgtgtgcc agacctcgcg cccggcggcc
     3061 tggtaggtgg gccaggcggc gagccggtcg gcgcccgcgc gcacctgctc cggatcgagc
     3121 cggggcgcca gcaccagcat gccgggcagt tgctcggcgg gcagcacatc gaccggcagt
     3181 tgcagcagtt cgtccaccac cgagggcggg gcctcggcgg cggggggcgc atcgggcggt
     3241 ttgggggcga agacgcccgg ccagcttttc caggccaggg tctccagcgc ggtgcccagc
     3301 gcggccagcc gctcctgcac ggcggtgtcg ggcagatcgg cggcggccac cgcggcggcg
     3361 agatcggcca gcgggggcag cggcgcggcg ggttcgagat agcccggctc gcgcccggcg
     3421 tcgagcgtgt cccagagcag cacgccgccc gcgaccggcc aggtctccca ggcccaccag
     3481 aggcgcacca gttcgagcgg gcgcgggcgg aacatccaga gcagcccggc ggccacgagc
     3541 agcgcgccgg cccagatgag cagcaccagc cccgccgcgc cgccctgccc gccggccagc
     3601 agcgcgacgg cggcgcccca gcagagcagc gtgaacagca cggcgatgat cgcgggcagg
     3661 cgcaggccgc ccaggaactc gcgccagacc tgggccagca tccaggcccc ggccagcggg
     3721 ccctcccagg cctcgggctc ggcgggcagg ccgggcggcg ggtcgtcgtc ggcgcgggcc
     3781 agggtggcgg gcaggtagag cgtcggcccc tcggtggccg gggtgacggg caggccgtcc
     3841 tcgctcgccg tgtccaccgg ctgcggcgcg gtgagcgtgg ccggggtctc ggcggcgcgc
     3901 agggcgtcct cggccagccc gcgcccgcgc cgggtcagca cgctgctcag ggcctggccg
     3961 ggcggctccg ctgtggcgtc cggcccgtcg gagtgccagg cgtggacgct ctcgctgccc
     4021 gccgtggtcc cgccgatacg caccggcgcg tccacgcgca cggcgcccgc ggcgctgccc
     4081 gccgccgtcg cgccgatctc caccgcgccg ttgagggcga gatcggcccc ggccagcagg
     4141 ccgcccgccg ccgcactgtc ggcggtgagg ccctccgccg ccgtggccca gccggtgagg
     4201 cgcgcgcccg gccccaggcg cacggtgccg ccctgcgcgc cgccgctgcc ctggatgcgg
     4261 ctcgcgcccc cggccaccag cgtgtcgccg gccagggcca tgccgcccac ctcggcgccc
     4321 gcgcccagcg tgaggtcgcc caggctgtag atgtcgccgt ccacccgcgc ccccgcctcc
     4381 acgaccacgt tgtgcccgaa caccgacccg gccacgcggg cgtcggcg
//
LOCUS       DASUYC010001080         2302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1571607, whole genome shotgun sequence.
ACCESSION   DASUYC010001080 DASUYC010000000
VERSION     DASUYC010001080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1571607"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VF276_17605"
     CDS             complement(<1..401)
                     /locus_tag="VF276_17605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6608719.1"
                     /translation="MSKDVAPGPAAGARGAAVSVPQAEFALEARDIVKRFPGVLANDH
                     VNFGLRHGEVHALLGENGAGKSTLMNILYGLYQPDEGQVLVNGEVVHFQNPRDAINRG
                     IGMVHQHFMLVPPLTVTENIMLGQESTRNGV"
     gene            complement(414..1322)
                     /locus_tag="VF276_17610"
     CDS             complement(414..1322)
                     /locus_tag="VF276_17610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6608720.1"
                     /translation="MTLCDQPILQLLLSVLVTALSFATPIILGALGGVACERAGVVNI
                     AIEGMMLSGAFGGYWAAVTFHSVPMGVLFAILIGGVMALLHGWLSITFKVDQIISGTV
                     INILGFGITSYLYGQWYARDAPASPGTIGSVPLPIPGISLSVQLLAIVAILLVAVLHL
                     VLNYTPWGLRVRAVGEHPKAADTVGISVHRVRYSAVLLGGLFAGLGGASYVPGYIPVF
                     TPGMTSGFGFIALAAMIFGKWKPFGALAASFFFGLTLSIQNNMQSCGVPIDARLVGML
                     PYIVTLFVLAGFVGRSIPPAADGVPY"
     gene            complement(1333..>2302)
                     /locus_tag="VF276_17615"
     CDS             complement(1333..>2302)
                     /locus_tag="VF276_17615"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6608721.1"
                     /translation="PEALAGTLIKSTPYIFAGLAVALAFKCGLFNIGAEGQVALGALF
                     AAFVGYNFQLPAIIHIPLTIAAGLLGGFIWGAIPGFLKARTGAHEVITTIMMNYLALQ
                     FVAWMLSGPMKKPGSVAAQTPYLQETSWLPMLIPQLQLHSGVLLALLTAALVWWFLYR
                     TTWGFEIRTTGANSSAARYAGIPIMRNIILAMALSGMLAGLAGAVEVTGVALDVTRLQ
                     PLGDRYFGLGFSSGYGFDSIAVALLGRSNPAGVVLAALLFGALRAGATQMQYETQVPG
                     EIISVIQGLILLFVAADAIIRAVYRVRATPASSGSPVLTSGWGEKA"
BASE COUNT          440 a          754 c          773 g          335 t
ORIGIN      
        1 atgacgccgt tgcgggtgct ctcctggccc agcatgatgt tctcggtcac ggtgagcggg
       61 ggcaccagca taaagtgctg gtggaccatg ccgatgccac ggttgatggc gtcgcgcggg
      121 ttctggaagt ggaccacctc tccattgacc agcacctgcc cttcatcggg ctggtagagg
      181 ccgtagagga tattcatgag cgtcgatttg ccggcgccgt tctcgcccag gagcgcgtgg
      241 acctcgccgt ggcgcaggcc gaagttcacg tgatcgttgg ccagcacgcc ggggaagcgc
      301 ttgacaatgt cgcgcgcctc cagcgcgaat tcggcctgcg gcacactgac cgccgcgccc
      361 cgcgccccgg cagcggggcc gggcgccacg tctttgctca tcgtcgctcc tgcctaatag
      421 ggcacgccgt cggcggcggg cgggatcgac cggcccacga acccggcgag cacaaacaac
      481 gtcacgatat agggcagcat gcccaccagc cgcgcgtcga tgggcacgcc gcacgattgc
      541 atgttgttct ggatcgagag cgtgagcccg aagaagaacg aggcggcgag cgcgccgaac
      601 ggcttccact tgccgaagat catcgcggcc agggcgatga agccgaagcc ggaggtcatg
      661 ccgggcgtga agaccgggat atagccgggc acgtaggacg ccccgcccag cccggcgaac
      721 agcccgccca gcagcacggc ggagtaacgc acgcggtgga cgctgatgcc gaccgtgtcc
      781 gccgccttgg ggtgctcgcc cacggcccgc acgcgcaggc cccagggcgt ataattcagc
      841 accaggtgca gcacagccac cagcaaaatt gccacaatcg ccagcaactg caccgacaac
      901 gatatgccgg ggatgggcaa cggcacgctg ccgatggtgc ccggcgaggc gggcgcatcg
      961 cgcgcatacc actggccgta caggtagctg gtgatgccga agccgaggat gttgatcacc
     1021 gtgccgctga tgatctgatc gaccttgaac gtgatcgaca gccagccgtg cagcaaggcc
     1081 attaccccgc cgatcaggat cgcgaacaac acgcccatcg gcacgctgtg gaacgtgacc
     1141 gcggcccagt aaccgccgaa ggccccgctc agcatcatgc cctcgatggc gatgttgacc
     1201 acgcccgccc gctcgcaggc cacgccgccc agcgcgccca ggatgatcgg cgtggcgaag
     1261 ctcagcgccg tgaccagcac gctcaggagt agttgtaaga tcggttgatc acagagagtc
     1321 actcgcttct ccctacgctt tctcgcccca gccgctggtc agcacggggg agccggagga
     1381 ggccggtgtg gcgcgcaccc ggtacaccgc acggataatg gcatcggccg ccacaaacag
     1441 caaaatcagg ccctggatca cggaaataat ctcgccgggc acctgcgtct catattgcat
     1501 ctgcgtggcc ccggcgcgca gggcgccgaa gagtagcgcg gccagtacca cgccggcggg
     1561 gttgctgcgg cccagcaagg ccacggcgat gctgtcgaag ccgtagccgc tggaaaagcc
     1621 cagcccgaaa tagcggtcgc ccagcggctg cagccgggtc acgtcgaggg ccacgccggt
     1681 cacttccacc gccccggcca gccccgccag catgccgctg agcgccatcg ccaggatgat
     1741 gttacgcatg atcgggatgc ccgcatagcg cgccgcggag gaattggccc cggtggtgcg
     1801 gatctcgaag ccccaggtgg tgcggtacag gaaccaccag acgagcgcgg ccgtcagcag
     1861 tgccagcaag acgccgctat ggagttgcaa ctgcgggatg agcatgggca gccacgaggt
     1921 ctcctgcagg tagggtgttt gcgcggctac gctgcccggc tttttcatcg gcccgctgag
     1981 catccaggcg acgaattgca gcgcgagata gttcatcatg atcgtcgtga tgacttcgtg
     2041 cgcgccggtg cgggccttga ggaagccggg gatggcgccc cagatgaagc cgccgagcag
     2101 cccggcggcg atggtcagcg gaatgtggat gatggcgggc aactggaaat tatagccgac
     2161 aaaggcggcg aacagcgcgc ccagcgccac ctggccctcg gcgccgatat tgaagaggcc
     2221 gcatttaaag gccagcgcca cggccaggcc ggcgaaaata tagggcgtgg acttgatcag
     2281 ggtgccggcg agggcttccg gt
//
LOCUS       DASUYC010001081         2393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_647660,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001081 DASUYC010000000
VERSION     DASUYC010001081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647660"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..374
                     /locus_tag="VF276_17620"
     CDS             78..374
                     /locus_tag="VF276_17620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608722.1"
                     /translation="MKKRGSLTLLQPDSPPDPPDDAAAAEQAPPLVHMRIVGRHGVFL
                     LERGANSNEKAVAVVDTRAPGGIHCTCGCRATDPASECEHVAVLRACGFLDSAA"
     gene            441..1304
                     /locus_tag="VF276_17625"
     CDS             441..1304
                     /locus_tag="VF276_17625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608723.1"
                     /translation="MEDHPRGTKVSPEIYVYATNKNFLNVYDALRIEKVKIEVAGYDQ
                     STGRQTGIASAWLDVDDVRLLTHLVTSRQFKDVLDRGRFEKFGGSDRDGAVESRTLVI
                     DWDPGQDGRFARYPYRITIANGPGQRTPNGAVQPKGDPASKLSMRLPEADMMKIMLTV
                     ADYLHAYETAHHHRIIAEKVREVQEKLAGRAERRLPVAAAPEDDERVATPAARSTNPP
                     LRVVPPADAPRNTPSRPTVVPPAANRPAAAPRSNPPIVAAAPNRPAPERPGRVAAESP
                     APRYERPTRTG"
     gene            1406..1834
                     /locus_tag="VF276_17630"
     CDS             1406..1834
                     /locus_tag="VF276_17630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011746086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX6608724.1"
                     /translation="MSRTLNKVSLIGRLGKDPEMRYTASGAAVTTFTLATNRMTRAAD
                     GTAQEETDWHTIVAWDKLAETCNQYLTKGRQVYIEGRLQTRTWEQDGQKRYKTEVIAH
                     EMLILDSKQDGNGNGAGNGYSRREALTVVAPADDADGLPF"
     gene            1942..>2393
                     /locus_tag="VF276_17635"
     CDS             1942..>2393
                     /locus_tag="VF276_17635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608725.1"
                     /translation="MATREPQSIGANALAATEAAVPAGVRWRAAVARAETAPPPQALG
                     LETIEGTLERITFQNEANGWTVARLHVTGQKAPVTIAGTMPTVHTGETLRLAGSWKQH
                     PQYGRQFQVAQYQALMPGTIPGLKKYLGSGLLKGVGPVWAEHLVNTFG"
BASE COUNT          458 a          794 c          812 g          329 t
ORIGIN      
        1 actataatgt gagaggaaca tttgttctaa agaccgcaga caccgccggt atgcggcaac
       61 gccgtgagga ggacaggatg aagaagcgag gatcgctgac gctgctgcaa ccggatagcc
      121 cgccggatcc gcccgacgac gctgccgctg ccgagcaggc gccgccgctg gtgcatatgc
      181 gcatcgtggg gcggcatggt gtgttcctgc tggagcgcgg agcgaacagc aacgagaagg
      241 cggtggccgt cgtcgatacc cgcgcccccg gcggcatcca ttgcacatgt ggctgccggg
      301 ccaccgaccc cgccagtgaa tgcgagcacg tcgccgtcct gcgtgcttgc gggttcctgg
      361 attccgccgc gtgaggctgg ggcggcaccg cgcgaagaac tatcaagaca gactgagatc
      421 gtaaaagcaa ggagcagcat atggaagatc atccacgggg cacgaaagtc tcgcccgaga
      481 tttacgtcta cgcgacgaac aagaatttcc tcaacgtgta tgacgccctg cgcatcgaga
      541 aggtgaagat cgaggtggca ggctacgacc agagcaccgg ccggcagacc ggcatcgcca
      601 gcgcctggct ggatgtggac gacgtgcggc tcctcacgca cctcgtgacc agccggcagt
      661 tcaaggatgt gctcgaccgc gggcggttcg agaagttcgg cggcagcgac cgggacggcg
      721 cggtcgagag ccgcacgctg gtcatcgatt gggatcccgg ccaggatggc cggttcgcgc
      781 gctatccgta ccgcatcacc atcgccaacg ggccgggcca gcgcacgccg aacggcgcag
      841 tacagccgaa gggcgatccg gcctccaagc tgtcgatgcg cctgcccgaa gccgacatga
      901 tgaagatcat gctgacagtg gccgattatc tccacgccta cgagacggcg caccaccacc
      961 gcatcatcgc cgagaaggtg cgcgaggtgc aggagaagct ggccgggcgg gccgagcgcc
     1021 gcttgccggt ggccgccgcg cccgaagacg atgagcgcgt agcgacgccc gccgcgcgga
     1081 gcacgaatcc cccgttgcgg gtcgtaccgc ccgccgacgc gccgcgtaac acgccgtcac
     1141 gcccgaccgt ggtgccgccc gccgccaacc ggcccgcggc ggcgccccgt tccaacccgc
     1201 cgattgtcgc cgctgccccg aaccgccccg ctcctgagcg gccgggccgg gtggcggccg
     1261 agtccccggc gcctcgttac gagcgaccca cgcgcaccgg atgaggcccg ttgccgcctc
     1321 tggcggtcct gagacatatc aagatctatg aagtagtgtt gagccacgtt ttgtgtgatt
     1381 agcttagaaa gagagaggta gagaaatgtc gaggacgttg aacaaagtca gcctgatcgg
     1441 tcgcctgggc aaagatccgg agatgcgcta taccgcgtcg ggcgcggcgg tgacgacctt
     1501 cacgctggcg accaaccgca tgaccagggc ggcggacggc acggcccagg aagagaccga
     1561 ctggcacacc atcgtcgcct gggacaagct ggccgagacc tgcaaccagt acctgaccaa
     1621 gggccgccag gtctacatcg agggccggct gcagacgcgg acctgggagc aggacggtca
     1681 gaagcgctac aagaccgaag tcatcgccca tgagatgctg atcctggact ccaagcagga
     1741 cggcaacggc aacggggccg gcaacggcta cagccgccgc gaggcgctga ccgtggttgc
     1801 cccggcggac gacgccgacg ggttgccctt ctaaggcgcg tgggggcggc gcggcgtggg
     1861 cgcgccgccc ctgttcgcgg aattgtctag ggagcgcagc gaagagtctc gtctccgcgc
     1921 cgggatcgta gatagtgacg catggctaca cgtgaacctc aatcgatagg cgcaaacgcc
     1981 ctggccgcca cggaagccgc cgtgcccgcc ggggtgcgct ggcgtgcggc ggtagcgcgc
     2041 gccgagaccg cgccgccgcc ccaggcgctg ggcctggaga cgatcgaggg cacgctggag
     2101 cgcattacct tccagaacga ggccaacggc tggaccgtgg cccgcctgca cgtcaccggg
     2161 cagaaagccc ccgtcaccat cgccggcacc atgccgactg tgcacaccgg cgagacgctg
     2221 cgcctggccg ggtcgtggaa gcagcacccg caatatggcc gccagttcca ggtcgcgcaa
     2281 taccaggcgc tcatgccggg caccattccc ggcctcaaaa aatacctcgg ctcgggcctg
     2341 ctcaagggcg tggggccggt gtgggctgaa cacctcgtta acaccttcgg ctt
//
LOCUS       DASUYC010001082         2117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1075311, whole genome shotgun sequence.
ACCESSION   DASUYC010001082 DASUYC010000000
VERSION     DASUYC010001082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075311"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VF276_17640"
     CDS             <1..401
                     /locus_tag="VF276_17640"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HEX6608726.1"
                     /translation="RDLLARPVHGILSTMLADGQPLSSLVWVDYEGVHIRINTALERR
                     KCRNMQANPMVTLLVVDPDRTDRWIEVRGRVAGITTDGAVAHADMLAQRYLGKQHFYG
                     DVYKAEWRQYETRVIVKIVPVKVSLDAIFR"
     gene            506..1726
                     /locus_tag="VF276_17645"
     CDS             506..1726
                     /locus_tag="VF276_17645"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX6608727.1"
                     /translation="MAIQQMPAPTRGDSHPATGPDPEACTPAFLAGDIIVFGSTGDLF
                     SNLSRWMMRGRHEEPTYAVHTGQFLDSQRVLEMAGMVGVAGFDTVLNRRYWHGPFRGL
                     CPPAAWAQLYTVLNRRFGRGGRTWQGLWKARGFEVWRSTRLTDTEREALTREALKYLD
                     VKFGFAKLWAHTLDDLICKLLRRDVFLFRHIDPEDRHPVCSGVTAAVYDRALHYRFGV
                     DPECVDPDQIHDWVQSPPDEWVCVFRASSEALAGDREMGRKMSVMGVGGEIAVPTLLY
                     TAGALAVSRRFRPKFRITRDSRALRLPGILLVAGGFSLNLAAAAAMLKAHQKDRLATG
                     GLYRLFRDPMYVLQIFLTLPGLFLLFNSWLVLAGVLPAYLAYRVFVREEHRYLADRFG
                     EAYREYLKTVLLKV"
BASE COUNT          360 a          689 c          697 g          371 t
ORIGIN      
        1 atcgcgacct cctggcccgc ccggttcacg ggatcttatc cacgatgctg gccgacggtc
       61 agccgctgtc gagcctggtg tgggtggact acgagggcgt gcacatccgc atcaacacag
      121 ccctggagcg ccgcaaatgc cggaacatgc aggccaaccc catggtgacg ttgctcgtgg
      181 tggacccgga ccggaccgac cgctggatcg aggtgcgtgg ccgggtcgcc gggataacga
      241 cggacggtgc ggtcgcccat gcggacatgc tcgcccaacg gtacctgggc aaacagcact
      301 tctatggtga tgtttacaaa gcggagtggc ggcaatatga aacgcgggtc atcgtcaaga
      361 tcgtgcccgt caaagtgtcg ctcgacgcca tcttcagata ggagcctgcg acgcgcccga
      421 tcgcctgccg cggcgctacg cggcgccgtg ccgagagcag cgcaccgggt aacgggcgac
      481 ggcgcaacga ctggagggac taatcatggc tattcagcag atgcccgcgc ccacgcgtgg
      541 ggacagtcac ccggctacag gaccggaccc agaggcgtgt actcccgcgt ttctcgcggg
      601 cgacatcatc gtcttcggga gcacgggcga tctcttcagc aatctgagcc gctggatgat
      661 gcggggccgg catgaggagc cgacctacgc ggtgcatacc ggccagtttc tcgattccca
      721 gcgcgtgctg gagatggcgg ggatggtcgg ggttgccggc tttgacaccg tgctcaaccg
      781 ccgctactgg cacggcccct tccgcgggct ttgcccgccc gcggcctggg cccagcttta
      841 taccgtgctc aaccggcgct tcgggcgcgg cgggcgcacc tggcaggggc tgtggaaagc
      901 gcgcggcttt gaggtctggc gctccacccg gctcacggat acggagcgcg aggctctcac
      961 ccgcgaggcg ttgaagtatc tggacgtgaa gttcggcttc gccaagctct gggcgcacac
     1021 actcgacgac ctgatctgca aactgctgcg ccgggacgtg ttcctgttcc ggcacatcga
     1081 ccccgaagat cgccaccctg tctgctccgg cgtcaccgcg gctgtctacg atcgggcgtt
     1141 gcattaccgg ttcggggtcg accccgaatg cgtcgacccc gaccagatcc acgactgggt
     1201 ccagtcgccc ccggacgagt gggtctgcgt atttcgcgct tccagcgaag ccttagcggg
     1261 agatagagag atgggaagga agatgtccgt tatgggagtc ggtggggaaa tcgccgtgcc
     1321 tacgttgctt tacacggccg gcgccctggc agtgagccgg cgcttccgcc cgaagttccg
     1381 cataacgcgc gactcgcggg cgctccgttt gcccggcatc ctgctggtgg ccggcggctt
     1441 ctccctgaac ctcgctgcgg cggcagccat gctgaaggct caccagaagg accgcctggc
     1501 caccggcggc ctctaccgcc tcttccgcga cccgatgtac gtgctccaga ttttcctcac
     1561 gctgccgggt ctgttcttgc tgttcaattc ctggctcgtg ctcgcgggcg tcctgcccgc
     1621 ctacctcgcc taccgcgtct tcgtgcgcga ggagcatcgc tatctggcgg acaggttcgg
     1681 ggaggcgtac cgggaatatc taaaaacggt tctgctcaag gtctgagcgg ttgccggcct
     1741 ggtgggcttg ggattaacgc ccgctcggtg ccgcgcggcg caaggatcgt aagccacagc
     1801 acgggtgcga ctggacggct atggtataat ggcaagacgg acccggcccg atgatgcgcc
     1861 gggcgcatgg gcgaccggtc aggctacatc cgccggccgg gcgcaatatt agcgggcggt
     1921 tggggagtgc tccgccgcga taaggaaaca ttgctcatgg atactgacgt tcgcctcgac
     1981 ccgcccaccg gcgagtccca cgccggcagc ggcacgctga ccattatccc ggcacagcgt
     2041 aaggcgcgca acccgaacat caaactggaa accgagcggg tcacgctcac gcgcaaacca
     2101 agctggctca acgtgaa
//
LOCUS       DASUYC010001083         3276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1146026, whole genome shotgun sequence.
ACCESSION   DASUYC010001083 DASUYC010000000
VERSION     DASUYC010001083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146026"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VF276_17650"
     CDS             complement(<1..422)
                     /locus_tag="VF276_17650"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX6608728.1"
                     /translation="MDRIWTVVQKELTDALRNRMTMATILISSVLFILLPLGMAYGMG
                     PNGLLQQLDSKQMDTADLGPILQVYPQLRTLDPASQLQGFMLSGIQALFLIIPLMVPM
                     VIAVYSVIGEKQNRSLEAVLATPIETSELLIAKCVAAAT"
     gene            complement(422..1390)
                     /locus_tag="VF276_17655"
     CDS             complement(422..1390)
                     /locus_tag="VF276_17655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011959285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6608729.1"
                     /translation="MTRPAAIAARDLRKKFDGRLAVDGVDLDVAPGEVFGFLGPNGAG
                     KTTTIRMLTALIAPTAGDAWIDGLALGHQDSAIRQRVGVLTETPGLYERMTARENLAF
                     FARLYRVPAAEIVQRIEFYLRRLDLWDRCDDRVGGFSKGMKQKVALARALLHQPPVVF
                     LDEPTAGLDPESSVVVHEFIDQIRGEGRTIFLCTHNLDEAERLCDRVAIFRRHIIRMG
                     SPMELRRSLYGRQVEIGLANPDAGDRLLAAARALPISTSATSDGDGRIIVSLQDLDTQ
                     IPALVTALVGAGAQITRVAEVEHSLQRAYLDLIRQAEAEPALQGVH"
     gene            complement(1387..>3276)
                     /locus_tag="VF276_17660"
     CDS             complement(1387..>3276)
                     /locus_tag="VF276_17660"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX6608730.1"
                     /translation="LVSGYFQGQEKRADEQRLALEELAALFKINSAANSTLDFDQVLD
                     LVVEHVTAAMHSDICSLFIYEPEIDRLVLRATRGLTPEAVGRVRLRLGEGVTGWAARA
                     GKPLALSNGWADERFLHVPALGEEETHAILAVPIVLFTKIKLVGVITLQSYDQRQFTE
                     RDISFLETVAGEVAIAIENARLHRDTDERLRQKVAELTILQGVSASIAATLNLQEVAH
                     LIATEAVHLLTADAAILYELREGQLVPRASFGFGYDGVVPVAGSTADSDAPPLPLPPP
                     LDPQTSLVGRAIMAGKPIGLQKQQGGGTETGHLLHTIGEGDALALQGVGMDAVAPLWV
                     DSEPDELPFASRICVPLLAPRSIVGGICVYSVAQRYFSEEQISLLLAFAREGAIAIEN
                     AHLYEEARHGMEMQTLLLREMHHRVRNNLQRTASLLRMQANRLPEDNVARHALQESII
                     RIQSIARVHDLLTRQQVGLTEVGAVANLACEVVNNTLGRPDLRVRWHVEAAPLTLSSR
                     EAFPLTLLLTELLSNSVLHGFHSREDGQVTIKAFSNANNVVIKITDNGQGLADDFDLG
                     RSSNLGLEVVRRLGEQELGGRLTIGPNPDGAGTRTVLTFPYQQPAGPAPTEADRVRES
                     AKAQA"
BASE COUNT          511 a         1072 c         1137 g          556 t
ORIGIN      
        1 gtcgctgccg ccacacactt agcgatcagc aactcgctgg tctcgatggg cgtcgccagc
       61 accgcttcca ggctgcggtt ctgcttttcg ccgatcacgc tgtacacggc gatgaccatc
      121 ggcaccatga gcgggatgat caggaagagc gcctggatgc ccgagagcat gaagccctgc
      181 aactggctgg ccgggtcgag agtccgcagt tgcggataga cctgcaggat tgggcccagg
      241 tcggcggtat ccatctgctt gctgtcgagt tgctgcagca ggccgttcgg ccccatgccg
      301 taggccatgc ccagcggcag gaggatgaag agcacgctcg atatgaggat cgttgccatc
      361 gtcatgcggt tgcgcagcgc atcggtcagc tctttttgca cgaccgtcca gatgcggtcc
      421 attaatgtac cccctggagc gcgggctcgg cttcggcctg gcggatgagg tcgagatagg
      481 cgcgctgcaa cgagtgctcg acttcggcca cgcgggtgat ctgcgcgccc gcgccgacca
      541 gcgccgtgac cagggccggg atctgcgtgt ccagatcctg caaactcacg atgatgcgcc
      601 cgtcgccgtc gctcgtggca ctggtgctga tgggcagggc gcgggcggcg gccagcagcc
      661 ggtcgccggc atcgggattc gccaggccga tctcgacctg ccgcccgtag aggctgcgcc
      721 gcagttccat cgggctgccc atgcggatga tgtggcggcg gaagatcgcc acccggtcgc
      781 acagccgctc ggcttcgtcc aggttatgtg tgcagagaaa gatggtgcgc ccctcgccgc
      841 ggatctgatc gatgaactcg tggacgacga ccgagctttc ggggtcgagg cccgccgtcg
      901 gctcgtcgag gaagaccacg ggcggctggt gcagcagggc acgagccaga gccaccttct
      961 gcttcatgcc tttggagaag ccgccgacgc ggtcgtcgca gcggtcccag agatcgagcc
     1021 ggcgcaggta aaattcgatg cgctggacga tctccgcggc gggcacgcga tagagccggg
     1081 cgaaaaaggc caggttttcg cgcgccgtca tgcgctcgta gagaccgggc gtctcggtca
     1141 gcacgccgac gcgctggcgg atggcgctat cctggtggcc gagggccagg ccatcgatcc
     1201 aggcgtcgcc cgcggtcggg gcgatcagtg cggtgagcat gcggatggtg gtggtcttgc
     1261 cggcgccgtt cggccccagg aagccgaaga cctcgccggg agccacgtcg agatccaccc
     1321 catccacagc gaggcgcccg tcgaatttct tgcgcaaatc gcgggcggca atcgcggcgg
     1381 gtcgggtcat gcttgcgcct tcgcggactc gcgcacccgg tcggcttccg tcggcgcggg
     1441 gccggcgggt tgctggtagg ggaaggtaag gaccgtgcgc gtgccggcgc catccgggtt
     1501 cggaccgatg gtcagccgcc cgcccagttc ctgctcgccc agccggcgca cgacctccaa
     1561 ccccaggttg gagctgcgcc caaggtcgaa gtcgtccgcc agcccctgcc cgttgtcggt
     1621 gatcttgatc accacgttgt tggcgttgct gaaggctttg atcgtgacct ggccatcctc
     1681 ccggctatgg aagccgtgca gcaccgagtt ggagagcaac tcggtcagca gcagcgtgag
     1741 cgggaaggct tcgcgcgagg agagggtcag cggcgccgcc tccacatgcc agcgcacccg
     1801 cagatcgggc cggcccagcg tgttgttgac cacctcgcag gccaggttgg cgaccgcgcc
     1861 gacctcggtc agccccacct gctggcgggt cagcaggtca tgcacgcggg cgatggactg
     1921 gatgcggatg atgctttcct gcagggcatg gcgcgcgacg ttgtcttccg gcagccggtt
     1981 ggcctgcatg cgcagcagcg acgcggtgcg ttgcaggttg ttgcgtacac ggtgatgcat
     2041 ctcgcgcagc agcagcgtct gcatttccat gccgtggcgg gcctcttcat atagatgtgc
     2101 gttctcgatg gcgatggcgc cttcgcgggc aaacgccagc agcaggctga tttgctcttc
     2161 gctgaaatag cgctgcgcca cgctgtagac gcaaatgccg ccgacgatgc tgcgcggcgc
     2221 cagcaacggc acgcagatcc ggctggcgaa cggcagctcg tcgggctcgg aatcgaccca
     2281 cagtggtgcc acggcgtcca tgcccacgcc ctgcagggcc agcgcgtcgc cctcgccgat
     2341 ggtatggagc aagtgccccg tttcggtgcc gccgccttgc tgcttctgca agccgatggg
     2401 cttgccggcc atgatggccc ggcccaccag gctggtctgc gggtcgagcg ggggcggcag
     2461 gggcaggggc ggagcgtcgc tatccgcggt gctgcccgcc acgggcacca cgccgtcgta
     2521 cccaaagccg aaggacgccc gcggcaccaa ctggccctcg cgcaactcat agaggatggc
     2581 ggcatcggcg gtcagtagat gcacggcctc ggtggcgatc agatgcgcca cctcttgcag
     2641 gttcagcgtg gccgcgatgc tggccgacac tccttgcagg atggtcagtt cggccacctt
     2701 ctggcgcagc cgctcgtcgg tatcgcggtg caggcgggcg ttctcgatgg cgatagccac
     2761 ctcgccggcc accgtctcca ggaaggagat gtcgcgctcg gtgaactggc gctgatcgta
     2821 gctttgcagc gtgatgacgc cgaccagctt gatcttggtg aagagcacaa tcggcacggc
     2881 caggatggcg tgggtctctt cctcgccgag ggcgggcaca tgcaggaatc gctcgtcggc
     2941 ccagccgttg gacagcgcga gcggcttgcc ggcgcgcgcc gcccagccgg tgacgccctc
     3001 gcccaggcgc aaccgcaccc ggcccaccgc ctcgggcgtc agcccccgcg tggcccgcag
     3061 caccagccgg tcgatctccg gctcataaat aaagagcgag cagatgtcgc tgtgcatggc
     3121 cgccgtgacg tgctccacga ccaggtcgag cacctggtcg aaatcgagcg tcgagttggc
     3181 ggcggagttg atcttaaaca gggcggcgag ttcttccagc gccaggcgct gctcgtcggc
     3241 ccgcttttcc tggccctgga agtagccgct gaccag
//
LOCUS       DASUYC010001084         2120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1333369, whole genome shotgun sequence.
ACCESSION   DASUYC010001084 DASUYC010000000
VERSION     DASUYC010001084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333369"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            809..>2120
                     /locus_tag="VF276_17665"
     CDS             809..>2120
                     /locus_tag="VF276_17665"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEX6608731.1"
                     /translation="MTALLQGIRQFRIGRRRPPAQAGSMAGSAPQRGVRPAIDIAPND
                     PLLAYFLTAQGAVEIEHLRLESPALDALRAEGVKLTVPLVSQGELIGVLNLGPRRSDQ
                     DYSSDDRGLLANLAAQAAPAVRVAQLVREQQEAARARERIEQELRVARLIQQTLLPKE
                     RPVLPGWKLNTYYQPARAVGGDFYDFLPLADGRLGIVIADVTDKGVPAALVMATTRSI
                     LRSMAPSGSPGVVLERVNDLLYPDIPEKMFVTCFYAVLDPVSGRLHYANAGHDLPYRR
                     GTRRVDELRATGMPLGLMPGMRYEEKELVLGFGETVLFYTDGLVEAHNAQREMFSFPR
                     LMALFATPQPDGDVIAFLLDELGKFTGTGWEQEDDVTLVTLQRTTGPGSSSEIAGPGD
                     GAAWGTGNGWRVLAEFTVPSQPGNERVAMEQVAEVVAGLDLPAPR"
BASE COUNT          336 a          704 c          732 g          348 t
ORIGIN      
        1 ctagcaggcg actgtctcct gatcgttacg acgaccgtta cgacccgccg aatgacgcgt
       61 cgcctcgtgg gcgcgcagtt tccccggtta tacgagtcgc gcggtgcagg gctacgccat
      121 aatggatcgc cgcgtcctcc cgtgcctgac gacggatgca tgccggcgga cacctctggc
      181 gaatgggtcg cgtgactgcc tgcgcggcgc tgccacaatc cgcgggatag ggcggcgttg
      241 aataaaagag ggttcgcaac caacagcgat gtgctacaat aggacatgcg aatccggacc
      301 catggcctgc ccgccgggtc cggcgccggg tagtaccggg tggcgcgcct ggagtggcgc
      361 cggcataggg ttcgcccctg gtcgacttac ggaataccgg gttccgcacc ggcccgcggc
      421 tgagattgcg cggcctgtcc tggtatctcg tacccttacc cttacccccg cggcctgtcc
      481 ctttcccata gcgcccgtcc gtgccggatc cccggaccgc ttggcttatc aaggccccca
      541 caagcgctcg tcctcacccg ccatgcacgc gcacccgctt gttcgcggcc ctggcgctgc
      601 gtggcctgag gtattcgatt agctcaggcc gggcggtttg tggcgcgcca ccagccacgc
      661 agtcaatgcc caggtaacac ggccggtacc ggccgtatcc gatcccgaca atactgagga
      721 ttcgtcgtgc cgtaacgatc gccgtaacga tcaggagacg atcgtccgct aggatagggt
      781 cagataccgc aaggaaacgg agtgtgagat gaccgcactg ctgcaaggga ttcgacagtt
      841 caggattggc cggcgccgcc cgccggcaca ggccgggagc atggcgggga gcgcgccgca
      901 gcgcggcgtc cggccggcga tcgatatcgc gccgaatgac ccgctgctcg cctacttcct
      961 gaccgcgcaa ggggcggtgg agatcgagca tctgcgcctc gaatcgccgg cgctcgacgc
     1021 gctccgggcg gagggagtca agctcacggt gccgctggtc agccagggcg agctgatcgg
     1081 ggtactcaac ctgggcccac ggcgcagcga ccaggactac tcctccgacg accgcggctt
     1141 gctcgccaac ctggccgcgc aggctgcgcc cgccgtccgg gtggcccagc tggtgcgcga
     1201 gcagcaggaa gcggcgcggg cgcgggagcg catcgagcag gagttgcgcg tcgcccgcct
     1261 gatccagcag accctgctgc ccaaagagcg gcccgtgctg ccggggtgga agcttaacac
     1321 ctactatcag ccggcccgcg ccgtcggcgg ggacttttat gacttcctgc cgctggccga
     1381 cgggagattg ggcattgtca tcgccgacgt caccgacaag ggtgtgcccg ccgcgctcgt
     1441 catggcgacc acgcggagca tcctgcgctc gatggctccg tccggctcgc cgggcgtggt
     1501 cctggagcgg gttaacgatc tgctctatcc cgacatcccg gaaaagatgt tcgtcacctg
     1561 cttctacgcc gtgctcgacc cggtcagcgg gcggctgcac tacgccaacg ccgggcacga
     1621 tctgccgtac cggcgtggta cgcgccgcgt cgacgaattg cgggcgacgg gcatgcccct
     1681 gggcttgatg ccggggatgc gctacgagga gaaagagctg gtgctgggtt ttggcgagac
     1741 ggtgctgttt tacacggatg gcctggtgga ggcgcacaat gcgcagcgcg agatgttcag
     1801 cttcccccgg ctcatggcct tgtttgccac cccgcagccg gatggtgacg taatcgcttt
     1861 cctgctggac gagttgggca agttcaccgg gacggggtgg gagcaagagg acgacgtgac
     1921 gctcgtcacg ctccagcgca ctaccgggcc gggcagcagc agcgagattg cggggcccgg
     1981 cgacggcgcc gcctggggga ccggcaacgg ctggcgggtg ctggccgagt tcaccgtgcc
     2041 aagccagccg ggcaacgagc gcgtcgcgat ggagcaggtg gccgaggtcg ttgccggtct
     2101 ggatctgccc gcgccccggc
//
LOCUS       DASUYC010001085         3356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_880532,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001085 DASUYC010000000
VERSION     DASUYC010001085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880532"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VF276_17670"
     CDS             <1..616
                     /locus_tag="VF276_17670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEX6608732.1"
                     /translation="DGALADWRIDLDAGTATLAEPSPGLGALSLPLAPMLGCFGVAPP
                     RGQAISTATSAEHGGNMDYPGFVAGVTVYFPVFVPGALFHLGDGHAIQGDGEIVGTGI
                     EISMEVQFTVDLRKGRPIGWPRGEDAEYIFTVGNARPLDQAVQHATTEMLRWLRDDYG
                     LDTRAASILLGQAARYDLGNVYDPAYTMVCKMPKRVLASLRGPA"
     gene            719..871
                     /locus_tag="VF276_17675"
     CDS             719..871
                     /locus_tag="VF276_17675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HEX6608733.1"
                     /translation="MSILRAILCIVFPPLAVIDKGCGSIVIVTLLTIAGWVPGVIGAI
                     FINTRK"
     gene            complement(949..1482)
                     /locus_tag="VF276_17680"
     CDS             complement(949..1482)
                     /locus_tag="VF276_17680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608734.1"
                     /translation="MLGKSLAEIGRYAEQYAVLHQDSVGAPPHAALVRYVKRQDLPAL
                     GLNCMVDWGVIEEPPYVLVVLQGSFQPNYPDAAAFRGPPAHYLAYIYDVWAGEVTYVD
                     ATADAGHFKKILNNPALPDPVAPASTNCPAFQGTRMHYGSTPPTPIMTALPYVPPPTH
                     IPGPVPTEAAPLPAPMK"
     gene            complement(1734..2555)
                     /locus_tag="VF276_17685"
     CDS             complement(1734..2555)
                     /locus_tag="VF276_17685"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6608735.1"
                     /translation="MTGTNAANQGPARATRRAPVVLEVAGQQVFGVIHRPATEAGRLP
                     GVAIFHGLVGSKDQPHQIFVKLAEALARAGMVALRIDFRGRGDSEGETVDITPAADLM
                     DARAALDYLAVQPDVDPARLALCGMSWGGAIAACLAGHDPRVSAVALWSSAPVAGFDW
                     NPPFRQVDGRDVAEMWGNIVGRQFYEGLGHIHPLADIQRARGPVLIAYGTTDEEVPQS
                     AVDHAAATLAAAGIACDVLAIPDADHAFMGYDWEQALIAGTVAWLRRALLDSTTR"
     gene            3030..>3356
                     /locus_tag="VF276_17690"
     CDS             3030..>3356
                     /locus_tag="VF276_17690"
                     /inference="COORDINATES: protein motif:HMM:NF037651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit beta"
                     /protein_id="HEX6608736.1"
                     /translation="MLDFLRHTPKNFTLSEADRARQREVPENLWVKCTRCKELSYVKE
                     FENSLKVCQKCGYHARLSAGERLQWLLDEDSFQETAGDIEPGDPLAFVSEGQGYRDKL
                     EEAQTKT"
BASE COUNT          593 a         1156 c         1069 g          538 t
ORIGIN      
        1 cgacggcgca ctggccgact ggcgcatcga cctggacgcc ggcaccgcta ccctggccga
       61 gccgtcgccc ggactcggcg cgctcagcct gccgctggcg cccatgctgg gctgcttcgg
      121 cgtggccccg ccgcgcggcc aggccatctc gacggccacc tccgctgagc acggcggcaa
      181 tatggactat cccggcttcg tcgccggagt cacggtctac ttcccggtgt tcgtgccggg
      241 cgcgctgttc cacctgggcg acggccatgc catccagggc gatggcgaaa ttgtgggcac
      301 ggggattgag atctcgatgg aggtgcagtt cacggtcgat ctgcgcaagg ggcgacctat
      361 cggctggccg cgcggcgagg acgccgagta catcttcacg gtgggcaacg cccggccgct
      421 cgaccaggcc gtgcagcacg ccaccaccga gatgctgcgc tggctgcgcg acgactacgg
      481 cctggacacc cgcgcggcga gcatcctgct gggccaggcc gcccgctacg acctgggcaa
      541 cgtctacgac ccggcctata ccatggtctg caaaatgcct aaacgggtgc tggcatccct
      601 gcgcggacct gcgtaagccg ggccggggcg cgtattgcca cgggccgccc cggcgttgcg
      661 tatatagagg aaagaggcgg cgccccggcg ccgggttgga acgagagaag gaagagttat
      721 gagcatactc cgcgcgatct tgtgtatcgt cttcccgccg ctggcggtca tcgacaaagg
      781 ctgcgggtcc atcgtgatcg tcaccctgct gactatcgcc ggctgggtgc caggcgtcat
      841 cggcgcgatc ttcatcaaca cgcgcaaata ggccgggcgc cggcccgaga acacgacagc
      901 ggcgcccgaa tgggcgccgc tgcaacattt aacagaccct ggggcaggtc acttcatcgg
      961 cgcgggcaag ggtgccgctt ccgtcggcac cgggccgggt atgtgggtcg gcggcggcac
     1021 atagggcagc gccgtcatga ttggggtggg cggtgtactt ccatagtgca tccgggtgcc
     1081 ctgaaaagct ggacaattgg tactggctgg tgccacagga tcgggtagtg ctggattgtt
     1141 caggatcttc ttaaagtgtc ccgcatctgc tgttgcatcg acataggtaa cttcgcccgc
     1201 ccacacatca taaatgtagg ccaggtaatg tgccggaggg cctctgaaag ccgcagcgtc
     1261 gggatagttg ggctggaacg atccttgaag aacaaccagc acataaggtg gttcctcgat
     1321 gactccccag tcgaccatgc agttcagtcc gagtgcgggc agatcctgcc gcttaacata
     1381 gcgtacaagc gccgcgtgcg gcggcgcacc tacactgtct tgatgaagta cggcatactg
     1441 ttcggcgtac cggccaatct ccgccaacga cttgcccagc aacgtgttcg gtcctagcgg
     1501 aatcggagta ccatcggtgc gcagaggaac cgggcttctg gtcgccgtgc tcatatcagt
     1561 agttacagga gttgcggttc ccgtcgccgc gtaaccggcg gaaccacagg cgctaagcgc
     1621 caaactcagg aggagtccgc cggctaagcg taagcccacc atcagctttg aacgtgaccg
     1681 catccgtctt cctctcagcg cgatagcgtg tgctgcaact cccatagctg caattagcgg
     1741 gtggtgctgt ccaagagcgc gcgccgcagc cacgccaccg tccccgcgat cagcgcctgc
     1801 tcccagtcgt agcccatgaa ggcgtggtcg gcatcgggga tggcgagcac gtcgcaggcg
     1861 atcccggcgg cggccagcgt ggcggcggcg tggtccacgg cggattgcgg cacttcctcg
     1921 tcggtggtgc cgtaggcgat cagcaccggc ccgcgcgccc gctggatgtc ggccagcgga
     1981 tggatatgcc ccaggccctc gtagaactgg cggcccacga tgttacccca catttcagcc
     2041 acatcccgcc catccacctg gcggaagggc ggattccagt caaaccccgc taccggcgcg
     2101 ctcgaccaga gcgctaccgc cgagacgcgc gggtcgtgcc cggccaggca cgccgcgatg
     2161 gccccgcccc agctcatgcc gcacagcgcc agccgggccg ggtccacgtc gggctggacg
     2221 gcgagatagt cgagcgccgc ccgcgcgtcc atcaggtcgg cggcgggcgt gatgtccacc
     2281 gtctcgcctt cgctgtcgcc ccggccccgg aagtcgatcc gcagcgccac catgccggcc
     2341 cgcgccagcg cctcggccag cttgacgaag atctggtgcg gctggtcctt cgagccgacc
     2401 aggccgtgga agatcgccac gcccggcagc cgcccggcct ccgtcgcggg ccggtgaatc
     2461 accccaaaca cctgttgccc ggccacctcc agcaccaccg gcgcccgccg ggtggcgcgg
     2521 gccggtccct gattggccgc gtttgtccca gtcatcgatt gtcctcctcc atacggcgcc
     2581 caccccgccg gcatatccgg ccccagccgc gtgcggcgcc caccatctcc ggaaatctac
     2641 atccatcata tacgctgcac ggcgccgcgc catccggcta cggaacgaga ttcgccgcgg
     2701 gccggtttga aaccggcccc tacaccgcga tagaggcgca ggccggtttc aaaccggccc
     2761 ctacagcgcg ttcgaggcgc gggtcggttt gaaaccggcc cctacagcgc aagccagaaa
     2821 cgcgcgatca cccataccac acgccaacgc ctataactga tcactgacca ctgaccactg
     2881 accactcgtc ccccgacggc ccgctttcac gaattgccca tagcctacag ccgtggtata
     2941 ataggggcgc gccttttgcc cgaggcccag acagcatggc tgggcgcaac gcggcgcggc
     3001 ccaggcgtga atacaggagg cacacccggt tgctcgattt cttgcgccac accccgaaga
     3061 acttcaccct ctccgaggcc gatcgcgccc gccagcgcga ggtgccggag aacctgtggg
     3121 tcaaatgcac ccgctgtaaa gagctaagct acgtcaaaga gttcgagaat tcgcttaagg
     3181 tctgccagaa gtgcggctat cacgcccggc tgtcggcggg cgaacggctg caatggctgc
     3241 tggacgagga cagcttccag gagaccgcgg gcgatatcga gccgggcgac ccgctggcct
     3301 tcgtctcgga gggccagggc taccgcgaca aactggagga agcccagacc aagacc
//
LOCUS       DASUYC010001086         2785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1111986, whole genome shotgun sequence.
ACCESSION   DASUYC010001086 DASUYC010000000
VERSION     DASUYC010001086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111986"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="VF276_17695"
     CDS             complement(<1..716)
                     /locus_tag="VF276_17695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608737.1"
                     /translation="MAMTPPPDLQTQLAAYQRRIRRDRRGQYLAGAMLAVAGYWLLVA
                     GLYYLDRPFPQWLPVGLAWTVLLPLAALLVEWQQRPTPAEIAAMLDARLDNRQRVLTA
                     VELLAAGAPAPLAATQLTTTAALLESAPADAVYPVHSPWPMAGFSAGLLALALGLLIL
                     KGVPDSFAPFQAGNGPDTAQAAAAALSPTPPDGLPGATPPTPPPAAATGATPSAGNAA
                     GDPGGTSGSATDPNAAMQQAA"
     gene            complement(707..2170)
                     /locus_tag="VF276_17700"
     CDS             complement(707..2170)
                     /locus_tag="VF276_17700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608738.1"
                     /translation="MNRAVAAIRIGVGCLGLLLLLAVPASPRVSAAEPDVPQLTTRYF
                     TIYYPAGEEQTANWYAGFVDDMDSTVSELLGADPVTGLILVIYNTEAEYTAANPLAEL
                     HPGVLAHAIPDRKEIGVAVERLRAAPPELARESFRHEMTHVVAGALSNQELPIGFQEG
                     LAQYNELSDSRAGEVVKALQAAQAQGVDLLSWDDLTNTRRFQRQLDVAYPESYTVMAF
                     LADRYGMGAYTRFLAGLRAGHAWSAALAEGFGVSAAELETQWRDWLPGFFTDGWRKNV
                     LRASDLAPGRTLYDAGQFAEAAAYFKQSEALYRDLGRMAQLTAAMNARVNAERAQQAT
                     QQAAAAGTALAAHDYAGAQQQATTAAATFAALQLTARRNQAAGIADQAAQGVQALATL
                     DRARLAQQAWRVADAEQAAREAGAALAALGDAPRVAEANRILADIWGLERAAGLAALG
                     AGALGLIGAALAAVRRRLRAARMPRPPVLPEETASWL"
     gene            2575..>2785
                     /locus_tag="VF276_17705"
     CDS             2575..>2785
                     /locus_tag="VF276_17705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6608739.1"
                     /translation="MEIEQTGTAKIRVLIVDDSSCMRDSLRTALSFDPALEIVGEATD
                     GAEAVKMAAHLQPDVALVDLEMPLGD"
BASE COUNT          418 a          932 c         1022 g          413 t
ORIGIN      
        1 gcggcctgct gcatggcggc attggggtcg gtcgcgctgc cggaggtgcc gccggggtcg
       61 cccgcggcgt tgccggcgga cggcgtggcg ccggtcgcag cggcgggcgg cggggtcggg
      121 ggcgtggcgc ccggcaagcc gtcgggcggg gtcggcgaga gggccgcggc ggcggcctgg
      181 gcggtatcgg ggccgttgcc cgcctggaac ggcgcgaagc tgtcgggcac gcccttgagg
      241 atgagcaacc ccagcgctag cgccagcaag cccgcgctga agcccgccat cggccagggg
      301 ctatgcaccg ggtagaccgc atccgccggg gccgattcca gcagcgccgc cgtggtcgtg
      361 agttgcgtgg cagccagcgg ggccggcgcg cccgccgcca gcagttccac cgccgtcagc
      421 acgcgctggc ggttgtcgag ccgcgcgtcg agcatcgccg cgatctccgc cggcgtgggt
      481 cgctgctgcc attcgaccag cagcgcggcc aggggcagca gcaccgtcca ggccaggccc
      541 accggcagcc actgggggaa cgggcggtcc aggtagtaca gccccgccac gagcagccag
      601 tacccggcca cggcgagcat ggctccggcc aaatactggc cgcgccggtc gcgccggatg
      661 cggcgctgat aggcggccag ttgcgtttgc aggtcgggcg gcggggtcat agccatgacg
      721 cggtctcctc tggtagcacc ggggggcgcg gcatccgggc ggcgcgcagg cggcggcgaa
      781 cggcggccag ggcggcgccg atcagtccca gcgcgcccgc gccgagcgcg gccaggcccg
      841 ccgcccgttc cagaccccag atgtcggcga gaatgcggtt ggcctcggcc acgcgcgggg
      901 cgtcgcccag ggcggccagc gctgcaccgg cctcgcgggc ggcctgctcg gcgtcggcca
      961 cgcgccaggc ttgctgcgcg aggcgggcgc ggtcgagcgt cgccagcgcc tgcacaccct
     1021 gcgccgcctg gtccgcgatg cccgccgcct ggttccggcg ggcggttaac tggagggcgg
     1081 caaaggtcgc ggcggcggtg gtcgcctgct gttgcgcgcc ggcatagtcg tgtgcggcca
     1141 gggccgtccc ggcggcggcg gcctgctgcg tagcctgctg ggcgcgctcg gcgttgacgc
     1201 gggcgttcat cgccgccgtc agttgcgcca tgcggcccag gtcgcggtag agcgcctcgg
     1261 attgcttgaa ataggcggcg gcctcggcaa actgcccggc gtcatacagc gtccgccccg
     1321 gcgcgagatc ggaggcgcgc aacacgttct tgcgccaacc gtcggtgaaa aagccgggca
     1381 gccagtcgcg ccactgcgtc tccagttcgg cggcggagac gccgaagccc tcggccagcg
     1441 cggcggacca cgcgtgcccg gcgcgcagcc cggccaggaa gcgcgtgtag gcgcccatgc
     1501 cgtagcggtc ggccaggaac gccatcaccg tgtagctctc cgggtaggcc acgtcgagtt
     1561 gccgctggaa gcggcgcgta ttggtcaggt cgtcccagga gagcagatcg acgccctggg
     1621 cctgggcggc ctgtagcgcc ttcaccacct cgccggcgcg gctgtccgac agttcgttgt
     1681 actgggccag cccttcctgg aatccgatgg gtagttcctg gttggacagc gcgcccgcca
     1741 ccacatgggt catctcgtgg cggaaactct cgcgggccag ttcgggcggc gccgcgcgca
     1801 atcgctcgac ggccacgccg atctctttgc ggtcggggat ggcgtgggcc agcacgccgg
     1861 ggtggagttc ggccaggggg ttggccgcgg tgtactcggc ctcggtattg tagatcacca
     1921 ggatcagccc ggtcacgggg tcggcgccga ggagttcgct gaccgtgctg tccatgtcgt
     1981 cgacgaagcc cgcataccag ttcgcggtct gctcttcgcc cgcggggtaa tagatggtga
     2041 aatagcgggt ggtgagctgg ggtacgtcgg gttccgcggc agacacacgc gggctcgcgg
     2101 gcacggccag taagagcaag agccccaggc accccacccc gatgcggatt gcggcaacag
     2161 cacggttcat gtctggtctg cgcattctag ggggtcgccg cagggagcga tcacccagga
     2221 gaggtcatta taccatatta tggcgtgatt gtactcctgt tttgcccgtg atcacgcatg
     2281 atttttcaga tcaaattcct gccgcctccg ccctccagtc cggccaatag ggcaaatcag
     2341 ccctgggcgg ggtccgcctc cagatgtaga cccgtccacc caaattccgc catatgagat
     2401 ccaacaaggg ccagatgccc gatcccgcag tctctgctaa gatattgatg acggcaacga
     2461 ggacacacaa tccgaaggtc gtaaaccaga gcaaattgca gtagacttcg cggcaacgcg
     2521 aacaggggcc ggccccccat aggccggaga gcaggaaaga tcagggtggc acagatggag
     2581 atcgagcaga cgggtaccgc gaaaattcgg gttttgattg tcgacgattc gtcctgcatg
     2641 cgcgacagtc tgcgcacggc cctgagcttt gacccggccc tcgaaatcgt cggcgaagcc
     2701 actgacggcg ccgaagcggt gaagatggcg gcgcatctcc agcccgacgt ggccctggtc
     2761 gatctcgaaa tgcccctggg cgatg
//
LOCUS       DASUYC010001087         2931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_649072,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001087 DASUYC010000000
VERSION     DASUYC010001087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649072"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1657
                     /locus_tag="VF276_17710"
     CDS             <1..1657
                     /locus_tag="VF276_17710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HEX6608740.1"
                     /translation="VMAEGGIAGALANLDKKDSWQVHFQDTMRGGAFLNNWRTAEILA
                     KEAPERVLELEQWGAVFDRTPEGLIMQRPFGGHTYRRLNHVGDRTGLELIRTLQDRAV
                     ATGMTCYMEVTMTRLLKDGDRVVGAFGYYRETGRFLAIKARAVVLVTGGWGRMFKVTS
                     NSWESTGDGAMLAFDAGAELMGMEMVQFHLTGMVWPPGVRGLLVTEGVRGEGGILRNS
                     KGERFMFDPKYTPASYKGRYADTEEEAGRWIEDKANNRPPPELLPRDVVARAIYREVL
                     AGNGTEHGGVYLDVTHRGADFIRRKLPSMYEQFHALGDVDITAQPMEVYPTIHYTMGG
                     VRAEPETAATTLPGLYAAGEVSAGLHGANRLGGNALCELLVFGKRAGEGAAQYAQSAG
                     PGTLDEAAIQEEAASLLAPFESSGTENPYVITEELQAAMQEGAMIARTEESLQKCLNT
                     VLELQQRAHNLHVEGSRVYNPGWHTARDVHNMLKISEIIVRCALERQESRGAQWRLDY
                     PNPDPEWAKKNLIATRQDDTVRITTTPVPAMPPELAGLFEEGH"
     gene            1658..2440
                     /locus_tag="VF276_17715"
     CDS             1658..2440
                     /locus_tag="VF276_17715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013988227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEX6608741.1"
                     /translation="MYMVTLRVFRGDASGGTERDYQVPVFPGMVVLDAIHHIQAHDDS
                     SLACRWNCKAGKCGSCSAEVNGRPRLMCKTRIADYTNSDAPIVVHPLKTFPLIKDLVT
                     DVSWNYRMSAKIAPFTPAADAPRPLVMQQIDVDRIQEFRKCIECFLCQDVCHILRDHN
                     RKDLFYGPRQIIKLANLDMHPYDSLDRRRMIREEAGVGVCNITKCCTEVCPEHIHITD
                     NGIIPLKERVADTFFDPVMWVARKFSRKVPPPPPQPAEQPAE"
     gene            complement(2493..2888)
                     /locus_tag="VF276_17720"
     CDS             complement(2493..2888)
                     /locus_tag="VF276_17720"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEX6608742.1"
                     /translation="MAFTSDLSGQEFWLVVDKGYIPMGLVLGNSVYSMGAMGGWLSGI
                     KGQFRGEVVEVTRLMYDAREMALQRMKAEAESLGADGVIGVELKIEYLHGGQWMEVTA
                     VGTAVKWVGMREPSRGQVVVGTSEQGRTL"
BASE COUNT          529 a         1032 c          936 g          434 t
ORIGIN      
        1 cgtcatggcc gagggcggca tcgccggggc gctggcgaac ctcgacaaaa aggactcctg
       61 gcaggtccac ttccaggata cgatgcgcgg cggcgcgttc ctcaacaact ggcgcacggc
      121 ggagatcctg gcgaaagagg cccccgagcg ggtgctggag ctggagcagt ggggcgcggt
      181 gttcgaccgc acgcccgagg gcctgatcat gcagcggccc ttcggcggcc acacctaccg
      241 ccgcctcaac cacgtcggcg accgcaccgg gctggaactg atccgcaccc tgcaggatcg
      301 cgccgtcgcc acggggatga cctgctacat ggaagtgacc atgacccggc tgctcaagga
      361 cggcgaccgg gtggtgggcg cgttcggcta ttaccgcgag acggggcgct tcctcgccat
      421 caaggcgcgg gcggtggtgc tggtgaccgg cggctggggg cgcatgttca aagtgacctc
      481 caactcctgg gagtccaccg gagacggcgc catgctggcc ttcgacgctg gagccgaact
      541 gatgggcatg gagatggtgc agttccacct caccggcatg gtctggccgc ccggcgtgcg
      601 cggcctgctc gtcaccgagg gcgtgcgcgg cgaaggcggc atcctgcgca acagcaaggg
      661 cgagcgcttc atgttcgacc ccaagtacac gcccgcctcc tacaaagggc gctacgccga
      721 caccgaggag gaggccggcc gctggatcga agacaaggcc aacaaccggc ccccgcccga
      781 gttgctaccg cgtgacgtgg tggcgcgcgc gatctaccgc gaggtgctgg ccggcaacgg
      841 caccgagcat ggcggcgtct acctcgacgt gacccatcgc ggcgcggact tcatccgccg
      901 caagctgccg agcatgtatg agcagtttca cgcgctgggc gacgtggaca tcacggccca
      961 gccgatggaa gtctacccga cgatccacta cacgatgggc ggggtgcgcg ccgagcccga
     1021 gacggcggcg acgacgctgc ccggtctcta cgcggcgggc gaggtgtccg ccgggctgca
     1081 cggtgccaat cgcctgggcg gcaatgccct gtgcgaactg ctggtgttcg gcaagcgcgc
     1141 gggcgagggc gcggcccagt acgcgcaatc cgccggcccc ggcacgctcg acgaagccgc
     1201 catccaggag gaagcggcct cgctgctggc ccccttcgag tcatcgggca ccgagaaccc
     1261 ctacgtgatc accgaggagt tgcaggcggc catgcaggag ggggcgatga tcgcgcgcac
     1321 tgaggagagc ttgcaaaagt gcctcaacac ggtgctcgaa ctccagcagc gggcgcacaa
     1381 cctgcatgtc gagggctcgc gcgtctacaa ccccggctgg cacacggcgc gcgacgtgca
     1441 caacatgctc aagatctcgg agatcatcgt gcgctgtgcg ctggaacgcc aggaaagccg
     1501 cggcgcgcaa tggcggctcg actatcccaa ccccgacccg gaatgggcca aaaagaacct
     1561 gatcgccacc cggcaagacg acaccgtccg gatcacgacg acccccgtcc cggcgatgcc
     1621 gcccgaactc gccgggctgt tcgaggaggg ccactaaatg tacatggtca ctctgcgggt
     1681 gttccgcggc gacgcatctg gcggtaccga gcgcgactac caggtgccgg tctttccggg
     1741 catggtagtg ctcgacgcga tccaccacat ccaggcgcac gacgacagca gcctcgcctg
     1801 ccgctggaac tgcaaggcgg gcaaatgtgg ctcgtgcagc gccgaggtca acgggcgccc
     1861 ccgcctgatg tgcaagaccc gcatcgccga ttacaccaac agcgacgcgc cgattgtggt
     1921 ccacccgctc aagacgttcc cgctgatcaa ggacctggtg acggacgtgt cctggaatta
     1981 ccgcatgtcg gccaagatcg cgccgttcac gcccgccgcc gacgccccgc gcccgctggt
     2041 catgcagcag atcgacgtgg accgcatcca ggagttccgc aagtgcatcg agtgcttcct
     2101 gtgccaggac gtgtgccaca tcctgcgtga tcataaccgc aaggatctgt tttacggccc
     2161 gcgtcagatc atcaagctgg cgaatctcga catgcacccc tatgacagcc tggaccgccg
     2221 ccgcatgatc cgcgaggaag ccggggtagg tgtctgcaac atcaccaagt gctgcaccga
     2281 ggtctgcccg gagcatatcc acatcaccga caacggcatc atcccgctca aggagcgcgt
     2341 ggccgacacc ttcttcgacc cggtgatgtg ggtggcgcgc aagttcagcc gcaaggtacc
     2401 accgccgccc ccgcagcccg cagagcagcc cgcagagtag cttccgcttc cgcagtgcac
     2461 ggcccgccgg cgaccccggc aggctgcgcc gatcacaagg tgcggccctg ctcagaggtg
     2521 ccgacgacca cctggccgcg gctcggctcg cgcatgccca cccatttgac ggcggtaccc
     2581 accgccgtca cctccatcca ctgcccgccg tgcagatact cgatcttcag ttccacgccg
     2641 atcacgccgt ccgcgccgag cgattcggcc tcggctttca tgcgttgcag cgccatctcg
     2701 cgggcgtcgt acatcagccg ggtgacttcg accacctcgc cgcggaactg gcccttgatg
     2761 cccgacagcc agccgcccat cgcgcccatc gagtagaccg agttccccag caccagcccc
     2821 atggggatgt agcctttgtc caccaccagc cagaattcct gccccgacag atccgatgtg
     2881 aaagccatgc gccgtcctcc ttatcgttcg cgtgcctgcg gaaattgtcg c
//
LOCUS       DASUYC010001088         2429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_687247,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001088 DASUYC010000000
VERSION     DASUYC010001088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687247"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VF276_17725"
     CDS             <1..205
                     /locus_tag="VF276_17725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018209957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608743.1"
                     /translation="QAILLAVVLREGAGLTAREIRAYCARRLDDFMVPQHVQIRAALP
                     RTDNGKVDRRRIQAEFDPCTTTS"
     gene            complement(169..876)
                     /locus_tag="VF276_17730"
     CDS             complement(169..876)
                     /locus_tag="VF276_17730"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6608744.1"
                     /translation="MQLLIIEDNPSTLKMLAFLLENEGYTVHAKSTVATGLEVLRREP
                     IDLLVLDVMLPDSNGLQVCRQIRNEGLNTPILMVSALGSRTDKLAALEAGADDYMTKP
                     LDTNEVVARVRALARRTQPTLAPELQTRLCVGPICLDVAQQCIVLASTGKVLPLTRME
                     VQLLHVLMRNAGNVVSHDRLITEAWGSNYEGSSNQLEVYVHRLRTKLRKEVGDQEVIR
                     TVPGGSYEVVVQGSNSA"
     gene            complement(1050..1733)
                     /locus_tag="VF276_17735"
     CDS             complement(1050..1733)
                     /locus_tag="VF276_17735"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX6608745.1"
                     /translation="MSEPTSAAGAGATRAPLITVLVAGTRAETGVTANLPPDQFQVWT
                     IQPAEAPSLLRAASPAVAILALDDPAHLDLVRELAGAAPPLLVLVPPEATDLVAAALA
                     AGAWDYVRRSAADLALLPVLLQRAIAGAGAQRRLVELDQQRTAAPSSPAEQEQKRRIG
                     RLAHDLRTPLTYLIGYSELLLTRDHPPAVVKQMAGEIFREAERMAAMVEGLRDMYANN
                     AGDAPLNHP"
     gene            complement(1896..>2429)
                     /locus_tag="VF276_17740"
     CDS             complement(1896..>2429)
                     /locus_tag="VF276_17740"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6608746.1"
                     /translation="VQQTIGMLRALVSDLRPPLLDTSGLLGALDYLVGRVGRETDLTI
                     SFTSRLGCRLDPTVEIVVFRLVQEALANIRKHAEAHHAWIRLQREDDELHLEVRDDGK
                     GFAVNESMNRALATGHLGLASMFERAEAAGGRLSIDSRVGEGTVLHFVLPFRPVEGDG
                     DPEPVVPTDADADAAQA"
BASE COUNT          357 a          794 c          864 g          414 t
ORIGIN      
        1 ccaggccatt ctgctggcgg tggtcttacg cgaaggcgcc ggcctcacgg cccgcgagat
       61 ccgcgcctac tgcgcccgcc ggttggacga cttcatggtg ccgcagcatg tgcagatccg
      121 cgccgcgctg ccgcgtaccg acaacggcaa ggtggaccgg cgccggattc aggccgagtt
      181 cgacccctgc accaccactt cgtagctgcc gcccggcacc gtgcggatca cttcctggtc
      241 gccgacctct ttgcgcagct tggtacgcag ccggtggaca tagacctcta gctggttact
      301 gctgccctcg tagttgctgc cccaggcttc ggtgatcagc cggtcgtgcg agacgacgtt
      361 gcccgcgttg cgcatcagca catgcaggag ttgcacttcc atccgggtca gcggcaacac
      421 cttgccggtg ctcgccagca cgatgcattg ctgggccaca tcgaggcaga tcgggccgac
      481 gcagaggcgc gtctggagtt cgggggccag ggtgggctgg gtgcgccgag ccagcgcgcg
      541 cacgcgggcg acgacctcgt tggtgtcgag cggcttggtc atatagtcgt cggcgccggc
      601 ctccagcgcg gccagtttgt ccgtgcggct gcccagcgcc gagaccatca ggatgggggt
      661 gttcaggccc tcgttacgga tctggcggca gacctgcaag ccgttgctgt cgggcagcat
      721 cacgtcgagc accagcagat cgatgggctc gcggcgcaag acctccagcc cggtggcgac
      781 ggtgcttttc gcgtggacgg tgtagccttc attttcgagc aggaaggcca gcattttaag
      841 cgtcgaagga ttatcctcga taatcagcag ttgcacagca agccccatta gattctgccc
      901 gcagatcggg cgcatcatac ctttctgtct tcattctaat tagcccccgc gtccttgtca
      961 acttgactta tctgttcctt acggcagccg ccgcgggcgg ctggccctgg cggcaaatcg
     1021 gataggcgcc gtggagttag ggatgaatgt caggggtggt tgagcggggc atcaccggcg
     1081 ttgttggcgt acatatcccg caagccctcg accatggccg ccatgcgctc ggcttcacgg
     1141 aagatctccc cggccatttg cttaaccacg gcgggcggat gatcgcgggt cagcagcagt
     1201 tcggaatagc cgatcaggta ggtcagcggg gtgcgcagat cgtgggctaa gcgcccgatg
     1261 cggcgcttct gctcttgctc agcggggctg ctcggcgctg cggtgcgctg ctggtctagc
     1321 tcgaccaggc ggcgctgggc tcccgcgccc gcgatggctc gctgcagcag gacgggcagc
     1381 agcgccagat cggcggcaga gcgccggacg tagtcccagg ccccggcggc gagcgcggcg
     1441 gccaccaggt cggtggcctc gggcggcacc agcaccagca gggggggtgc ggcgcccgcc
     1501 aactcgcgca ccaggtcgag gtgcgccggg tcgtccagcg cgagaatggc gaccgcgggc
     1561 gaggcagcgc gcagcaggct gggcgcctcg gcgggctgga tggtccagac ctggaactga
     1621 tcgggcggca ggttggccgt tacgccggtc tcggctcgcg tgccggcgac cagcacggta
     1681 atcagcggcg cgcgggtggc cccggcgccg gctgcgcttg ttggctcgct catcctctat
     1741 ctccgcaatg gctgtgggcg cgtcgccggc catccccgga aaccgttgga cgcggggtca
     1801 ggagtctcgg tccacccggt gtggcgggca catgtcgcgt gcgggccgcc gggggagtga
     1861 aacgcgggag taggaaacgc ccctgcgggc gcggctcacg cctgggccgc atcggcatcc
     1921 gcgtcagtgg gcaccaccgg ctcggggtcg ccgtcgccct ccacggggcg gaacggcaag
     1981 acgaagtgca gcaccgtgcc ttcgcccacc cggctgtcga tggacaagcg cccgcccgcg
     2041 gcctcggcgc gctcgaacat gcttgccagg cccaggtggc cggtcgccag cgcgcggttc
     2101 atgctctcgt tgactgcaaa gcccttgccg tcgtcgcgga cttcgagatg gagctcgtcg
     2161 tcctcgcgct gcagccggat ccaggcatgg tgggcttcgg catgtttgcg aatgttcgcg
     2221 agcgcttcct ggacgaggcg gaacacgacg atctcgaccg tggggtcgag ccggcagccc
     2281 aggcggctgg tgaagctgat ggtgaggtcg gtttcgcggc ccacccgccc gacgaggtaa
     2341 tcgagcgcgc ccagcaggcc gctggtgtcg agcaggggcg ggcgcagatc gctgaccagg
     2401 gcgcgcagca tgccgatcgt ctgttgcac
//
LOCUS       DASUYC010001089         2408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1042563, whole genome shotgun sequence.
ACCESSION   DASUYC010001089 DASUYC010000000
VERSION     DASUYC010001089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042563"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VF276_17745"
     CDS             complement(<1..475)
                     /locus_tag="VF276_17745"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX6608747.1"
                     /translation="MNRAGLGGVAVACVCVVAAAGAPLLAPYDPGLLTGPPLALPSAA
                     HLLGTNDVGQDILSRLLYGVRTSLLIAGSVAGLSAGLSWAVGLAAGMSRLAETLLMPL
                     TELLLALPSLPLYLLTVTLVGPSLTNLVLTLALLSWPSFARIVRSLVLAARGAPYV"
     gene            complement(468..1469)
                     /locus_tag="VF276_17750"
     CDS             complement(468..1469)
                     /locus_tag="VF276_17750"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6608748.1"
                     /translation="MSRAVARLTVRYALLVACVILLNFLIPRLLPGDPLNVGAAEGLE
                     SAVPLSAAARAQLRSYYHLDAPLPGQFVAYLGDLAHGNLGWSIARSAPVGELLLARLP
                     WTLALLLTALLLAAALGTALGMLAGRQPGSRRDRTLVALTAALSALPEFLIAIGLLLV
                     FAITLRWFPLLGGQTAFADYGTDALAGGRAALDMAWHLTLPALTLVLAGGAGFVLIAR
                     DVTAGITRAPWLTVARAKGLPESRIAWGHMLPNLALPLLTYFGLRLGAVLGGALVVER
                     VFNVPGLGLLGYEAIRARDYPVLQALFLLAGLGVLLANFAVELLYLRLARRQPAAHE"
     gene            complement(1632..2381)
                     /locus_tag="VF276_17755"
     CDS             complement(1632..2381)
                     /locus_tag="VF276_17755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6390 family protein"
                     /protein_id="HEX6608749.1"
                     /translation="MSDLLTPAPPLAGQVRFIHYAFMPNRLHYCGGDDNPTLLDYGTT
                     QAVDAGLTPLLRRFTGALPYLRLIAQTNGIADPFDARVVEAYWVGNDLLERVEVRQLY
                     DALAERFGKQLEGRAREWVLGKAPAGARPHHNFHVFDIHSRVGEVEHGLETMDQCRIS
                     AGKVLAVEGAELVVERQPLILQEGKLALGPGQRERVVRQVDTRGFADTAQVGDWVALH
                     WGWVCEVLTPRQAANLAHYTRYHLALANQTI"
BASE COUNT          383 a          856 c          884 g          285 t
ORIGIN      
        1 ccacgtaggg cgcgccgcgc gccgccagca ccaggctgcg cacgatgcgc gcgaacgacg
       61 gccaggacag cagcgccagc gtcagcacca ggttggtgag gctcggcccg accagcgtca
      121 ccgtgagcag atacagcggc aggctgggca gagccagcag gagttcggtc agcggcatca
      181 gcagggtctc ggccaggcgc gacatcccgg cggccaggcc cacggcccaa ctcagccctg
      241 ctgagagccc ggccacgctc ccggcaatga gcagcgaggt ccgcacgccg tacagcagcc
      301 ggctcaatat gtcctggccc acgtcgttgg tgcccagcag gtgggcggcg ctgggcagcg
      361 ccagcggcgg gccggtcagc aggccggggt cgtagggggc cagcagcggc gcgcccgcgg
      421 cggcgaccac gcacacgcag gcgacggcga cgccgcccag cccggcccta ttcatgcgcg
      481 gcgggctgcc gccgggccag ccgcaggtag agcaattcga cggcgaagtt cgccagcagc
      541 acgcccagcc cggccagcag gaacagcgcc tggaggaccg ggtagtcccg cgcccggatg
      601 gcctcgtaac ccagcagccc cagccccggc acgttgaaca cgcgctctac gaccagcgcc
      661 ccgcccagca cggcgcccag gcgcagcccg aaataggtca gcaagggcag cgccagattg
      721 ggcagcatgt gcccccaggc gatgcgcgat tcgggcaggc ccttggcccg cgccacggtc
      781 agccagggcg cgcgcgtgat gcccgccgtc acgtcgcggg cgatcagcac gaagcccgcg
      841 ccgcccgcca gcaccagcgt gagggccggc agggtcaggt gccaggccat gtcgagcgcg
      901 gcccgcccgc ccgccagggc gtccgtcccg tagtcggcga aggccgtctg cccgcccagc
      961 agcgggaacc agcgcagcgt gatggcgaac acgagcagca ggccaatggc gatcaggaac
     1021 tcgggcaggg ccgaaagcgc cgccgtcagc gcgaccaggg tgcgatcgcg ccggctgccg
     1081 ggctgccggc ccgccagcat gccgagggcc gtgcccagcg ctgccgccag cagcagggcg
     1141 gtgagcaaca acgccagggt ccagggcagc cgggccagca gcaactcgcc gaccggagcc
     1201 gaccgggcga tggaccagcc caggttgccg tgtgccaggt cgcccaggta ggcgacgaac
     1261 tggccgggca gcggcgcgtc aagatggtag taggagcgca gttgcgcccg cgcggcggcg
     1321 gagagcggca ccgcggactc caggccctcg gcggcgccca cgttgagcgg gtcgcccggc
     1381 agcaggcgcg ggatgaggaa attgagcagg atgacgcagg ccacgagcaa tgcgtagcgc
     1441 acggtcaggc gggcaacggc gcgcgacata gccgcccgtc agcgaccgcg cagggcgcgc
     1501 ccgtcgttgg ggcagggcac ctcgctcatc ggcccgcccg ccgtcgccgt gggcctgaac
     1561 gtgcctgggt cgtatacccg gtcgaaccgg cggtggtaac gcccaatcgt cggcagttcc
     1621 tctccagaca atcagatcgt ctggttggct agcgccagat ggtagcgcgt gtagtgggct
     1681 agatttgccg cctggcgcgg ggtgagcacc tcgcagaccc agccccagtg cagcgccacc
     1741 cagtcgccca cctgcgcggt gtcggcgaag ccacgcgtgt ccacctggcg caccacccgc
     1801 tcgcgctggc ccggccccag cgccagcttg ccctcctgca ggatgagcgg ctgccgctcg
     1861 accaccagtt cggctccttc caccgccagc accttgcccg cgctgatgcg gcactggtcc
     1921 atcgtctcca ggccatgctc cacctcgccc acgcggctgt ggatatcgaa gacatggaaa
     1981 ttgtggtgcg ggcgcgctcc ggcgggcgcc ttgcccagca cccattcgcg cgcccggcct
     2041 tcgagctgtt tgccgaagcg ttcggccagc gcgtcgtaga gctggcggac ttcgacccgc
     2101 tccagcaggt cgttgccgac ccagtaggct tcgacgacgc gggcgtcgaa ggggtcggcg
     2161 atgccgttgg tctgggcgat gaggcgcagg tagggcaggg cgccggtgaa gcggcgcagc
     2221 aggggggtga ggccggcgtc cacggcttgc gtggtgccgt agtcgagcag ggtcgggtta
     2281 tcgtcgccgc cgcagtagtg caggcggttg ggcatgaagg catagtggat gaagcgcacc
     2341 tggccggcca ggggcggcgc gggggtcagc aggtcactca tccgttgcgt cctcctggga
     2401 cgcgagtt
//
LOCUS       DASUYC010001090         2366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1804558, whole genome shotgun sequence.
ACCESSION   DASUYC010001090 DASUYC010000000
VERSION     DASUYC010001090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1804558"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..1081)
                     /locus_tag="VF276_17760"
     CDS             complement(146..1081)
                     /locus_tag="VF276_17760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6608750.1"
                     /translation="MDFALVISVLAAAIAAGTAILYACLGEILCERAGVLNLGVEGMM
                     LMGALSGFAVTYWTASPWLGALAALVMGGLLSLIHAVLTVTLRANQVVSGLALTLFGT
                     GLTSFLGQPLVGQPAPASFEKVALPLLGDLPVLGPILFRQDMLTYISFLLVPALWVLI
                     YRTRAGLNLRAVGENPATTDAMGLSVAGLRYLYIAAGGMLAGLGGAAISLASNPGWTE
                     NMTAGRGWIAIALVIFATWSPARAALGAYLFGGVEALQFRLQSAGTQVSPFFLNMLPY
                     LFTILVLVFATRETVRRRIGAPAALGLPYYREERS"
     gene            complement(1089..2207)
                     /locus_tag="VF276_17765"
     CDS             complement(1089..2207)
                     /locus_tag="VF276_17765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004573018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6608751.1"
                     /translation="MATSRIRIERQAAPSRAAAVLIPVVAVLLALGLGAGLLLAVGKD
                     PVAVYGAMFQGAFGSVNGLAETLVKTIPLLLAGLGVSIAFRMLLWNIGAEGQLYLGGI
                     AATGTALYLLPDAPGVVLIPAMIVAGFAGGAAWALIAGALRAYLKVNEIITTLMLNYV
                     AILFSQYLVHGPWKNPQGYGFPGTPPLPDAAWLPHLGTTRVHLGLLFGVLAAVILFVI
                     LRRTWWGYEIRVIGENERAARYAGINITRNILLVMALSGGLAGLAGMSEVAGIAHQLQ
                     TNLSPGYGYTAIIVAWLAKLNPWTIIVVSFLFAGLLVGGDQLQISMGLPAAIAPMLQG
                     TILFFLLGSDILARYRIVWTRRPAPTVAAEAQPGKKVG"
BASE COUNT          414 a          783 c          835 g          334 t
ORIGIN      
        1 atgagttctt tttgcgatcc tgattggaag ctagggtggg gatggccttc ctaatatggc
       61 gtttccggct gactcctggt tctaccatat tagaatctct gcgtcctctg cgcctcggcg
      121 gtgtcgttct cctgccgggg agagctcagg accgttcttc gcggtagtag ggcaggccga
      181 gcgcggcggg ggcgccgatg cgccgccgga cggtctcgcg cgtggcgaac accaggacga
      241 ggatggtgaa cagatagggc agcatgttca ggaagaaggg cgagacctgg gtgcccgcgc
      301 tttgtaggcg gaactgcaag gcttccacgc cgccgaacag gtaggcgccg agggcagcgc
      361 gggcgggact ccaggtggcg aagatgacaa gggcgatggc gatccagccg cgccccgccg
      421 tcatgttctc ggtccagccg gggttcgacg ccagcgagat ggccgcgccg cccagcccgg
      481 ccagcatgcc gcccgccgcg atatagaggt agcgcagccc ggccacgctc aggcccatgg
      541 cgtcggtcgt ggccggattc tcgcccacgg cgcgcaggtt cagcccggcg cgggtgcggt
      601 aaatcagcac ccacagcgcg ggcaccagca ggaacgagat gtaggtgagc atgtcctggc
      661 ggaacagaat cgggccgagt accggtaggt cgccgagcag cggcagcgcc accttctcga
      721 acgacgcggg cgcgggctgg cccaccagcg gctggccgag gaacgaggtc agcccggtgc
      781 cgaacagcgt gagggccagg ccgctgacca cctggttggc gcgcagggtc acggtcagca
      841 cggcatggat cagactgagc aggccgccca tcaccagcgc ggccagcgcg cccagccagg
      901 ggctggcggt ccagtaggtc acggcgaagc cgctgagggc gcccatgagc atcatgccct
      961 cgacgccgag gttcagcacc cccgcccgct cgcagaggat ctcgcccagg caggcgtaca
     1021 ggatggccgt gccggcggca atcgccgcgg ccaggaccga gatcaccaac gcaaagtcca
     1081 tggtctcctt acccgacctt cttgcccggt tgcgcctcgg cggcgaccgt cggggccggg
     1141 cggcgcgtcc agacgatgcg atagcgggcc aggatgtcgc tgcccagcag gaagaacagg
     1201 atggtgcctt gcagcatcgg cgcgatggcc gcgggcaggc ccatgctgat ctgcaactgg
     1261 tcgccgccca ccagcagccc ggcgaatagg aacgagacga cgatgatggt ccacggattg
     1321 agcttggcga gccaggccac gataatcgcc gtgtagccgt agccgggcga caggttggtc
     1381 tgcaactggt gggcgatgcc cgcgacctcg ctcatgccgg ccaggccggc caggccgccg
     1441 ctgagcgcca tcaccagcag gatgttgcgc gtgatattaa tgcccgcgta gcgcgcggcg
     1501 cgttcgttct cgccgataac gcggatctcg tagccccacc acgtgcgccg caggatgacg
     1561 aacaggatca ccgccgccag cacgccgaac agcaggccca ggtggacgcg ggtggtgccc
     1621 aggtgcggca gccacgccgc gtcgggcagg ggcggcgtgc cggggaagcc gtagccctgc
     1681 gggttcttcc acggtccgtg caccaggtac tgcgagaaca ggattgccac gtagttcagc
     1741 atcagcgtgg tgatgatctc gttgaccttc aggtaggcgc gcagcgcccc cgcgatcagc
     1801 gcccaggccg cgccgcccgc gaacccggcg acgatcatgg ccgggatgag cacgaccccc
     1861 ggcgcgtccg gtagcaggta gagcgcggtg ccggtggcgg cgatgccccc caggtacaac
     1921 tggccctcgg cgccgatgtt ccacagcagc atgcggaagg cgatgctgac ccccagcccg
     1981 gccagcagca gcgggatggt cttgaccagg gtctcggcca gcccgttcac cgagccgaac
     2041 gcgccctgaa acatggcgcc gtagacggcg accgggtctt tgcccacggc cagcagcagc
     2101 ccggcgccca gccccagggc cagcagcacc gccaccacgg ggatgagcac cgccgccgcc
     2161 cgcgaggggg cggcctggcg ttcgatccgg atgcgcgaag tagccatacg tctatcctgt
     2221 gcgagccgtc agcaatcagc cgtcagccgt caggttgcta gcctgcggca ttttgggcgt
     2281 caggtgaggc aattggtcag cgagcggaaa gttatcgtta gcggcggcag gagcgataaa
     2341 tcgcgccgct acgtcagccg cataca
//
LOCUS       DASUYC010001091         5413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_187300,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001091 DASUYC010000000
VERSION     DASUYC010001091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5413
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187300"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3451
                     /locus_tag="VF276_17770"
     CDS             <1..3451
                     /locus_tag="VF276_17770"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6608752.1"
                     /translation="RYGGSVIGFSGDAILAWFDGDDGHRAVTCALALQAAFVPVAGVP
                     LPAARQVTLGLKVAVAAGAARRFLVGDPAIRMIEVLAGAVLDRLAAAERLARQGEVVL
                     DPLTAACLRSRVRVAATRPGPRGAGPFVVVGGLARAAAPAPWPPLSPGALTPAQVRPW
                     LLPAVHAWLVSGQGDFLTELRPAVALFLRFAGLDYDHDAATGARLDAYIRWVQGILAR
                     YDGSLIQVTIGDKGSYLYAAFGAPLAHADDARRAVAAALELRAPPADLAYITGSAIGI
                     SGGLARTGTYGGSTRTYGVLGDAVNLGAGLMQAAAAGQVLLSGDLAGAVERSYTLHAV
                     PRVPVKGPRATVPVLAVVGPRPTPAIRLQEPEYTHPLVGRAAELAHVAQLTARVLAGQ
                     GRILGLVAEAGMGKSRLVAEIIRRAMAAGMQGYGGAAQATGTRIAYLAWHAVWRAFFD
                     LDPAAAPDVQVAELEAAVAGVDPALRRRVPLLAPALGLSLPDNDLTRDMDAQLRKESL
                     EALLVDCVKHRAQITPLLLVLEDAHWLDALSADLLGAIGRAIGALPVLVLLAYRPPDS
                     TGGPALPVLALPYAGELRVGELSGAEVAELIADRLAAAGHPPALPAVLVDAITTRAAG
                     NPFYIEELLNYLQDRGIALTDMANMDVMQLPDSLQSLILSRIDTLTVEQQTTLKVASI
                     IGRVFPAAWLWRVHPDLGPVERVQADLRALAALDLTPLETPEPDPRYLFKHIITQEVT
                     YASLPFALRARLHEQLATWLESSADAANPPLDLLAYHFGRSANTAKQHEYYRKAGDAA
                     AARFANAAAVQYYENLLALLPAHEQGPVHVLLGDMQERLSAWDAAETHYRAALTLVGG
                     VANAVHHAQASLGLGAVERARGAYSAAVIWLEQARREFADLGRQDGLSLVLIHLAIVY
                     WVQGKLDQTQALVDEILALEETTGDKWRISRALHLLGNVDLSRGDYPMAREWWVRSLV
                     LKRELGDKPGIAGSVTNMALAAFNEGQYTEARTLVTESLALYQELGSRREYGQARGVL
                     ARIHLAEGDFTTARSLFAANLILFRDLGARQEIVVSLIGLAAATEPAESRYAVRLIAA
                     ALALLAAIDSAIEPGDQLRADAVVAAAHARLGADGVREAWAQGQAMAWASAVDYALAP
                     GDG"
     gene            complement(3459..3761)
                     /locus_tag="VF276_17775"
     CDS             complement(3459..3761)
                     /locus_tag="VF276_17775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608753.1"
                     /translation="MSAQQHSVPGTFIRGADGALYFVPDSELESYRVPESGRPAMEEF
                     LKSHKHPERGGQAHPHSLEATHLHVPGPTAALGATGMSSSGHRAAIPPPEETEPAK"
     gene            complement(3767..5188)
                     /locus_tag="VF276_17780"
     CDS             complement(3767..5188)
                     /locus_tag="VF276_17780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfuA-like protein"
                     /protein_id="HEX6608754.1"
                     /translation="MTVYIFTGPTLAPAAARQELDAVYLPPVAQGDVYQAARRRPQAI
                     GIIDGFFDSVPAIWHKEILWAMREGIHVFGSASMGALRAAELAAFGMEGVGAIFAAYQ
                     SGALEDDDVVAVIHGPAESGYRPLSVALVNIRATLHAAAAGGIIVPATAVALERLAKD
                     LFYPERTYPRLLGAAAGHGLPAADLAALRAWLPGGQVDQKAEDARAMLRVIRERLAAG
                     MAPKQVYYHFEHTVFWDQARAGGRPPAPQPDAAHLPGDILLEELQLDPAAYAAVRQAT
                     LAHVLARTLAAQQGQAVPDDFTRAITEAFRRARGLFQPEATLQWMATHDLAPAQFDRL
                     MREEALLRWAVDWAGPEVSAHLADQLRLTAGYPALRDRARDKQRTLAAAGLADPNLAD
                     AGVTQDGLLDWYFTERTGAPPPADLTQYALDAGFLDEYAFIRAVLREFCYTRLIDSTT
                     PGAYTTRTLWPTQPADPDHAAGG"
     gene            complement(5185..>5413)
                     /locus_tag="VF276_17785"
     CDS             complement(5185..>5413)
                     /locus_tag="VF276_17785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002611293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HEX6608755.1"
                     /translation="AAGPTRQTDSCAADVTWELARLQAAGITRVVVVDLTKPEFRLPV
                     VRVIIPGLESLAGTASYVPGARAHATAGRPV"
BASE COUNT          752 a         1832 c         1956 g          873 t
ORIGIN      
        1 tcgttacggc ggcagcgtga tcggcttcag tggcgatgcc atcctggcct ggtttgacgg
       61 cgacgatggc caccgcgcgg tgacctgtgc cctggccctg caagcggcct tcgtcccggt
      121 ggccggggtg ccgctgcccg ccgcccggca ggtcacactg ggactcaagg tcgcggtggc
      181 ggcgggcgcg gcgcggcgtt tcctggtggg tgatcccgcc atccggatga tcgaggtgct
      241 ggccggggcg gtattggatc gcctggccgc cgcggagcgc ctggcccggc aaggcgaggt
      301 cgtgctcgat cccctgactg ccgcctgcct gcgcagccgg gtgcgggtcg ctgcgacccg
      361 tcccggtccg cgcggggccg gcccgttcgt cgtggtcggg gggctggcgc gggccgcggc
      421 cccggcgccc tggccgcccc tgtctcccgg tgcgctcacg cccgcccagg ttcggccctg
      481 gttgctcccg gcggtgcatg cctggctggt gagcgggcag ggcgactttc taacggaact
      541 ccgcccggcc gtcgccctgt ttctccgctt cgccgggctg gactacgacc acgacgcggc
      601 caccggcgcc cgcctggacg cgtacatccg ctgggtccag gggatactgg cccggtatga
      661 tggcagcctc atccaggtga cgatcggtga caagggcagc tatctgtacg cggctttcgg
      721 cgcgcccctg gcgcacgccg acgatgcgcg tcgggccgtg gcggcggccc tcgaactgcg
      781 tgccccgccc gccgacctgg cttacatcac cgggtcggcg atcgggatca gcggcgggct
      841 ggcgcggacg ggcacctatg gcggcagcac gcgcacctac ggcgtcctgg gcgatgctgt
      901 aaatctgggt gccgggctga tgcaagcggc ggcggccggg caggtgttgc tcagcgggga
      961 tctggccggc gcggtggagc ggtcgtatac cctccacgcc gtcccccgcg tgcccgtcaa
     1021 aggcccgcgt gccacggtgc cggtgctggc cgtcgtgggg ccccggccca ccccggccat
     1081 ccggttgcag gagccggaat acacccaccc gctggtcggg cgcgccgccg aactcgcgca
     1141 tgtggcgcaa ctcaccgcac gggtgctggc tggacaaggt cggatcctgg gcctcgtggc
     1201 cgaggccggc atgggcaaat cccgcctggt cgccgagatc atccgccggg ccatggcggc
     1261 gggtatgcag ggctatggcg gtgccgccca ggccaccggg acgcgcatcg cgtacttggc
     1321 ctggcacgcc gtctggcgcg ctttcttcga tctggacccc gccgccgcgc cggacgtgca
     1381 ggtcgccgag ctcgaagccg ccgtagccgg cgtcgatccc gcgctgcggc ggcgggtgcc
     1441 gttactcgca ccggccctgg gcctgtccct gcccgacaac gacctgacgc gggacatgga
     1501 cgcccagttg cgtaaagagt ccctggaggc gctgctggtg gattgcgtca aacaccgcgc
     1561 gcagatcacc ccgctgctgc tggtcctgga agatgcacac tggctcgacg cgctttcggc
     1621 ggacctgttg ggcgctatcg ggcgggcgat cggcgcgctg ccggtgctcg tgctgctggc
     1681 ctaccgtccg cccgactcta cgggtgggcc cgccttgccc gtgctggctt tgccgtatgc
     1741 cggcgaactc cgggtcgggg agttgagcgg cgccgaagtg gccgaattga tcgccgatcg
     1801 cctggccgcc gccgggcacc cgccggcgct gccggcggtg ttggtcgacg ccatcacgac
     1861 acgcgccgcg ggcaatccct tctacatcga ggaactgctc aactacctgc aggatcgcgg
     1921 gatcgccctg accgatatgg cgaacatgga cgtgatgcag ttgcccgaca gcctgcaaag
     1981 cctgattttg agccgcattg acaccttgac ggtggagcag caaaccacgt tgaaggtcgc
     2041 cagtatcatc gggcgggtct tcccggcggc ctggctttgg cgggtgcatc cggacctggg
     2101 gccggtcgag cgtgtgcagg ccgacctgcg cgccctggcc gcgctcgacc tcacgccgct
     2161 ggagacaccg gagcctgatc cacgctatct gttcaagcac atcatcaccc aggaagtgac
     2221 ttacgccagc ttgccgttcg ctctgcgcgc acggctgcac gagcaactgg cgacctggct
     2281 cgaaagcagt gcggacgccg ccaacccgcc gctggatctg ctggcctatc attttggccg
     2341 cagcgccaac acggccaagc agcacgagta ttaccgcaaa gcgggcgacg ccgccgcggc
     2401 ccggtttgcc aacgccgccg ccgtgcaata ctacgagaac ctgctggcgt tgctgcccgc
     2461 ccatgagcag gggccggtgc atgtgctgct gggcgacatg caggaacgcc tcagcgcgtg
     2521 ggatgccgcg gaaacgcact accgcgcggc actcaccctg gtcggcggcg tggcgaacgc
     2581 cgtgcaccac gcccaggcat ccctcggtct gggcgcagtg gagcgggccc gtggcgccta
     2641 ttctgcggcg gtgatctggt tggagcaggc acgccgcgag ttcgcggacc tggggcgcca
     2701 ggatggcttg agcctggtct tgatccacct ggcgatcgtc tactgggtgc agggtaaact
     2761 ggaccagacg caggcgctgg tggatgaaat cctggcattg gaggagacga cgggtgataa
     2821 gtggcgcatt tcgcgggcct tgcatctgct gggcaatgtg gacctgtcgc gcggagacta
     2881 cccgatggcg cgcgaatggt gggtgcggag cctggtcctg aaacgcgaac taggcgacaa
     2941 gccggggatt gccggttccg tcaccaatat ggccctggcc gcttttaacg aggggcagta
     3001 cacggaggcc cggacgctgg tgacggaaag cctggccctg taccaggagc ttggctcccg
     3061 ccgggagtac ggccaggctc gtggtgtgct ggctcgtatc catctcgccg agggggactt
     3121 caccacggcc cggagcctgt ttgccgcaaa cctcatcctg tttcgagatt taggcgcccg
     3181 gcaagagatc gtggtatcct tgatcgggct ggcggccgcc accgagccgg cggagagccg
     3241 ctacgcggtg cgcctgatcg ccgcggccct ggccttgctg gcggccattg acagcgcgat
     3301 cgagccgggc gatcagctgc gggcagacgc ggtggtcgcc gctgcgcacg cgcgattggg
     3361 cgcggacggg gtgcgcgagg cctgggcgca gggccaggcc atggcctggg cctcagccgt
     3421 ggactatgcg ctggcgcccg gcgacggctg aggcccgatt acttggccgg ttccgtctcc
     3481 tctggcggcg ggatagcggc ccgatgcccg ctactgctca tacctgtcgc ccccagggcc
     3541 gccgtggggc cggggacgtg gagatgggtt gcctccaggc tgtgtggatg cgcctgcccg
     3601 ccgcgttcgg ggtgcttgtg ggatttcaga aactcttcca tcgcgggccg gcccgattcc
     3661 ggcacgcggt aagattccag ttcgctgtcg ggaacaaagt aaagtgcgcc gtcggccccg
     3721 cggatgaatg tgcccggtac gctatgctgc tgtgcggaca tgttctctat cctcctgctg
     3781 catgatcggg atctgccggc tgtgtcggcc acaatgtgcg cgtagtatac gcgccaggcg
     3841 tagtggagtc aatcagtctc gtatagcaaa actcgcgcag caccgcgcgg ataaatgcat
     3901 actcgtccag gaatcccgca tccaaagcgt actgggtgag atcggcggga ggcggagcgc
     3961 cggtgcgctc cgtgaaatac cagtccagca gcccgtcctg ggtcacgccc gcatccgcca
     4021 ggttggggtc cgccaggccc gccgcggcca gggtgcgctg tttgtcgcgg gcccggtcgc
     4081 gcagcgctgg atagccggcg gtaagccgca actggtcggc caggtgtgcg ctgacctcgg
     4141 ggccggccca atcgacggcc cagcgcagca gcgcctcttc tcgcatcagc cgatcgaact
     4201 gcgccggggc caggtcgtgc gtcgccatcc attgcagggt cgcctcgggc tggaacaggc
     4261 cgcgggcgcg gcggaaggct tccgtgatcg cgcgggtgaa atcgtcgggg acggcctggc
     4321 cttgctgggc ggccagcgtc cgcgccagca catgggccag cgtggcctgg cgcaccgccg
     4381 catatgcggc cgggtcgagt tgtaattcct ccagcaggat atcgcccggc aagtgggcgg
     4441 catcgggctg cggggccggg gggcggccac cggcccgtgc ctggtcccag aagacggtat
     4501 gctcgaaatg gtagtagacc tgcttgggcg ccatcccggc ggccaggcgc tcccggatga
     4561 cgcgtaacat ggcccgcgcg tcctcggctt tctggtcgac ttggccgccc ggcagccagg
     4621 cgcgcagggc cgcgaggtcg gcggcgggca ggccgtgccc ggcggcggca ccgagcaggc
     4681 gcgggtaggt gcgctccgga tagaagaggt ctttagccag ccgttccagg gccacggcgg
     4741 tagccggtac aataatgccc ccggccgccg cggcgtgcag cgtcgcgcgg atattgacca
     4801 gcgcgactga taacgggcgg tagccgcttt ccgccgggcc gtggattacg gccaccacgt
     4861 cgtcatcctc cagcgcgccg ctctggtagg cagcgaaaat cgcgccgacc ccctccatgc
     4921 cgaaagccgc cagttccgcc gcccgcagcg cgcccatgct ggcgctgccg aagacatgga
     4981 tgccttcgcg catcgcccag aggatctcct tgtgccagat ggcggggacg gagtcgaaga
     5041 agccgtcgat gatgccgatg gcctgcggcc ggcggcgcgc cgcctgatag acatcgccct
     5101 gcgcgacggg cggcaggtag acggcgtcga gttcctggcg cgcggcggcg ggcgccaggg
     5161 tcggcccggt aaagatatag acggtcatac gggccggccc gccgtggcgt gtgcgcgcgc
     5221 ccccggcaca tagcttgcgg tgccggccag gctctccagg ccggggatga tgacgcggac
     5281 caccggcagc cggaactccg gtttcgtcag gtcgaccacc acgacgcggg tgatccccgc
     5341 cgcctgcagg cgggccagct cccaggtcac gtcggcggcg cagctatcgg tttgccgggt
     5401 cggtcccgcc gcg
//
LOCUS       DASUYC010001092         2627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1935891, whole genome shotgun sequence.
ACCESSION   DASUYC010001092 DASUYC010000000
VERSION     DASUYC010001092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1935891"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VF276_17790"
     CDS             complement(<1..352)
                     /locus_tag="VF276_17790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608756.1"
                     /translation="MWENRRFSGTGAWPRALVAGLAALVLATVLAWAVALAAPSRTVV
                     QVTLTADGAVARVNDDRLTLKLAPAEAAAGGHFGWYLAAPSDSLYYTPADPQTPLVSW
                     GRLGEALQLARPRAY"
     gene            579..2030
                     /gene="glnA"
                     /locus_tag="VF276_17795"
     CDS             579..2030
                     /gene="glnA"
                     /locus_tag="VF276_17795"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003263708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX6608757.1"
                     /translation="MVLISDEKALFGATSPDEVFELCRANGIKMVDVKFTDLPGGWQH
                     FSVPVDELNEGCFTEGLGFDGSSIRGFKHIHESDMLLRLDPTTAFVDPVLRVPTLSII
                     GDVYDPISGQPYGRDPRRIARRAEQYLQDTGIATTSYWGPEAEFYIFNSVRFDQTSNS
                     GYYFIDSDEGIWNSGKDGANQGFRPRYKEGYFPVPPTDKLQDLRSEIVLALQDAGIRV
                     EVQHHEVGTAGQAEIDMRFDTLVNMADKVMKYKYIVKNVCAQAGYVASFMPKPLFGDN
                     GSGMHVHQSLWNVEQPLFADRDGYAGLSETARWYIGGLLKHAPALLALAAPTTNSYRR
                     LVPGFEAPINLVYSQRNRSACVRIPMYSQNPKAKRIEFRAPDPSCNPYLTFAALLLAG
                     IDGVRNQIEPPRPIDEDIYELDPAEKLDIRSTPGSLDAVLDALAADHAFLLEGDVFSA
                     DLIDAWISYKREHELRPVQMRPHPYEFFLYSDV"
     gene            complement(2148..>2627)
                     /locus_tag="VF276_17800"
     CDS             complement(2148..>2627)
                     /locus_tag="VF276_17800"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX6608758.1"
                     /translation="ICCSERVLDDPEGSADFTFRLDPQDPDLAIRDDGVRFRLAPGAR
                     AVLADLHARGVRLAMASWNHVPPVRNLLAAFGLLPLFSQVVGGWTSDKATMLRSILAA
                     EAAAGRPTPPDSVLFVDDDPEARYRPMAAAVGTRFAQMGNPAEVASFADIARLVGVD"
BASE COUNT          481 a          874 c          858 g          414 t
ORIGIN      
        1 agtaggcgcg cggacgggcc agttgcaggg cttcgcccag gcggccccaa ctgacgagcg
       61 gggtctgggg gtcggcgggc gtgtagtaga gcgagtcgct gggcgcggcc aggtaccagc
      121 caaaatgccc gcccgccgcc gcctcggcgg gcgccagctt gagggtcagg cggtcgtcgt
      181 tcacgcgggc cacggcgccg tcggcggtca gcgtgacctg aaccacagtg cggctgggcg
      241 cggccagggc cacggcccag gcgaggaccg tcgccagcac gagcgccgcc agcccggcca
      301 ccaatgcacg gggccacgca ccggtgccgc taaaccttcg attttcccac atacccgcct
      361 agcataacac aaacgggtaa aaacatcctg gaccgggagt tttgttcatt gtgcacaagt
      421 tttacggcgc acagattgtt tgtgctgccg tagactcgtt tctcgaaagg atcgatagta
      481 ggaaacggag agatatttgt gcagagcgac catgaccctg tgaccggcgc ctgcacgcga
      541 tcttcgcgtc gttgcagtta gggagggaat aacagcgtat ggtactgata agcgacgaaa
      601 aagcgctctt tggcgcgaca agccccgacg aagtcttcga gttgtgccgc gccaacggga
      661 tcaagatggt ggacgtgaag ttcaccgatc tgccgggcgg ctggcagcac ttcagcgtgc
      721 cggtggacga actgaacgaa ggctgcttca ccgaggggtt gggcttcgac ggctcgtcca
      781 ttcgcggctt caagcatatc cacgaatccg acatgctgct gcgcctcgac ccgaccacgg
      841 cctttgtcga tcccgtgctg cgcgtgccga ccctgagcat catcggcgat gtctacgacc
      901 cgatcagcgg ccagccgtat ggccgcgatc cccggcgcat cgcccggcgc gccgagcaat
      961 atctccagga caccggcatc gccaccacca gctactgggg gccggaagcc gagttctaca
     1021 tcttcaactc ggtgcgcttc gaccagacct cgaactcggg ctactacttc atcgacagcg
     1081 atgagggcat ctggaactcg ggcaaggacg gcgccaacca gggcttccgc ccgcgctaca
     1141 aagagggcta cttccccgtc ccgccgaccg ataagctgca ggacctgcgc tcggagatcg
     1201 tgctggcctt gcaagacgcg ggtatccgcg tcgaggtcca gcaccacgaa gtgggcaccg
     1261 ccgggcaggc cgagatcgat atgcgcttcg acaccctggt caacatggcc gacaaggtga
     1321 tgaagtacaa gtatatcgtc aagaacgtct gcgcccaggc gggctacgtc gccagcttca
     1381 tgcccaagcc gctcttcggc gacaacggct caggcatgca cgtgcaccag agcctctgga
     1441 acgtggaaca gccgctgttc gccgataggg acgggtacgc aggtctcagc gagaccgcgc
     1501 gctggtacat cggcgggctg ctgaagcacg cgcccgcgct gctggcgctg gccgcgccga
     1561 cgacgaacag ctatcgccgt ctggtgcccg gcttcgaggc gccgatcaac ctggtctact
     1621 cgcagcgcaa ccgctcggcc tgcgtgcgca tccccatgta ctcgcagaac ccgaaagcca
     1681 agcgcatcga gttccgcgcg cccgacccgt cgtgcaaccc gtacctgacc ttcgccgccc
     1741 tgctcctggc cggcatcgac ggggtgcgca accagatcga gccgccgcgc cccatcgacg
     1801 aggacatcta cgagctggac ccggcggaga agctggacat ccgctccacg cccggctcgc
     1861 tggacgccgt gctggacgcc ctggccgccg accatgcctt cttgctggaa ggcgacgtgt
     1921 tcagcgccga cctgatcgac gcctggatca gctacaagcg cgagcacgag ctacggcccg
     1981 tgcagatgcg cccgcacccc tacgagttct tcctctactc ggacgtgtag gtcagcgcgg
     2041 caacgacgtt tattcaccgc gaagacgcga agggcgcgaa gaatggttct attaacgcca
     2101 atcttcgcgt tcttcgcgtc ttcgtggtga gatacagagt cggggattta atcgacgccg
     2161 accaggcggg cgatatcggc gaagctggcc acctcggcgg gattgcccat ctgggcgaag
     2221 cgcgtgccga cggcggcggc catcgggcga tagcgcgcct cggggtcgtc gtccacgaag
     2281 aggacgctgt ccggcggggt tgggcgaccg gcggcggcct cggcggcgag gatgctgcgc
     2341 agcatggtcg ccttgtcgct ggtccacccg cccaccacct ggctgaacag cggcagcagc
     2401 ccgaaggcgg ccagcaggtt gcggaccggc ggcacgtggt tccaactcgc catcgccagc
     2461 cgtacgccgc gggcgtggag gtcggccagc actgcgcggg cgcccggcgc cagccggaag
     2521 cgcaccccgt cgtcgcggat cgccaggtcg ggatcctgcg gatcgaggcg gaaggtgaaa
     2581 tccgccgagc cctcgggatc gtcgagcacg cgctccgagc agcagat
//
LOCUS       DASUYC010001093         2948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_2035918, whole genome shotgun sequence.
ACCESSION   DASUYC010001093 DASUYC010000000
VERSION     DASUYC010001093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2035918"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1589)
                     /locus_tag="VF276_17805"
     CDS             complement(<1..1589)
                     /locus_tag="VF276_17805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608759.1"
                     /translation="MLVAPRHDAQSGAELSNPPASASHRLSAAGSNVATAYRRATPAL
                     AWAVVPLLAVLAYLAVLRIGFLSDDFLTLITGRNRGLNPRILLPDPNWMFYRPISTIL
                     TWGLGWRLWGDNPFPYHLTGLLLHAGVALLLGLWLAGVSRRPGLGRLAGALFAVFPLH
                     TEAVGWLAAQWDLWAALFGLAALWLFTRWWAESRRVAIYLTALICWGLGVFSKESVIP
                     LLPLFALSIWAAPGRLAARDWRRLGGALVPFALIVTVNIGIRLATWGTLGGYAVPAGD
                     RILFWDQALNYMRLLLAPLNALVFGDPVVQIVGAVTSLLLLAGLIRYGNGLGRLLALS
                     GAWIALTLAPVLNLPVGRADLQNNRFLYLAAAGYCVALALLLCAALAAPGARPRLGRE
                     LIAGLLVISGLACWVQLQPWHTATVRAEGLIQALHRLIPPGPHKDAMTWYVENLPDSY
                     RGAYMFRLGFDVARYFADGDLPRIEAVPSAAQVDLAAAEPSGFALRFATDEVAGHAYI
                     GYAAGITAARPPPSTADAGADL"
     gene            complement(1606..2052)
                     /locus_tag="VF276_17810"
     CDS             complement(1606..2052)
                     /locus_tag="VF276_17810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608760.1"
                     /translation="MTPKLYSALKWGALFGGALALFETLGYLTSLRSQNLADYQITEC
                     LSLMLEAALPFVAGLLAARATGERVSGLVAGITAGVIVALINTVTEIIVPADALVQLV
                     DLPADAAQPTILDALFFRVTTLGLGAWFGWMGGRMGVLVSRRDSDH"
     gene            2325..2651
                     /locus_tag="VF276_17815"
     CDS             2325..2651
                     /locus_tag="VF276_17815"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX6608761.1"
                     /translation="MQNGLSESAPRTQLESWGAALALKPYLHAIADPTRLSMLHELAQ
                     AGEHQVLELARLLGLSQPLTSWHLRILKQAGLVSTRRLGRQVLCRLNRERLSEFKAQF
                     DALINL"
     gene            2726..>2948
                     /locus_tag="VF276_17820"
     CDS             2726..>2948
                     /locus_tag="VF276_17820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEX6608762.1"
                     /translation="MGKVRVAIIGVGNCASSLVQGVHFYRNAEDDEFIPGLMHVNLGG
                     YHVGDVEFSAAFDVDSRKVGTDLSEAIFAE"
BASE COUNT          565 a         1023 c          966 g          394 t
ORIGIN      
        1 agatccgcgc ccgcgtcggc ggtggacggc ggtgggcgag cggcggtgat cccggcggca
       61 tagccgatat acgcgtgacc ggcgacctcg tccgtagcga aacgcagcgc gaacccactc
      121 ggctcggcgg cagcgagatc gacctgggcg gccgagggca ccgcctcaat gcgcggcagg
      181 tcgccgtcgg cgaagtagcg ggccacatcg aaccccaggc ggaacatata ggcgccgcga
      241 taggagtccg gcaagttctc gacgtaccag gtcatggcgt ctttgtgcgg gccgggcggg
      301 atcaggcggt ggagggcctg gatcagccct tcggcgcgca ccgtggcggt gtgccagggc
      361 tggagttgca cccaacaggc cagcccgcta atcaccagca gccccgcgat gagctcccgc
      421 cccaggcgcg ggcgcgcgcc cggtgcggcc agggcggcgc acagcaagag ggcaagcgcc
      481 acgcagtagc ccgccgccgc caggtagagg aagcggttgt tttgcagatc cgcgcgcccc
      541 acgggcaggt tcaataccgg ggccagcgtc agcgcgatcc acgcacccga cagcgccagc
      601 aggcgcccga ggccgttgcc gtaccggatc aggcccgcga gtagcagcag actcgtcacc
      661 gcccccacga tctgcacgac cggatcgccg aacacaagcg cattgagcgg cgcgagcagc
      721 agacgcatgt agttcagcgc ctggtcccaa aagaggatgc gatccccggc aggcacggca
      781 tatccgccca gtgtgcccca ggtggcgagc cggatgccga tattcaccgt cactatcagc
      841 gcgaaaggca ccaacgcccc gcccagccgc cgccaatcgc gcgccgccag gcggcccggc
      901 gctgcccaga tcgacagcgc aaacagcggc agcagcggga tcacgctttc cttggaaaac
      961 acccccaggc cccagcagat gagcgcggtc agatagatcg cgacccgccg cgattccgcc
     1021 caccagcgcg tgaacagcca gagcgccgcc agcccgaaga gcgccgccca gagatcccac
     1081 tgcgcggcca gccagcccac ggcctcggtg tgcaacggaa acactgcgaa cagcgccccg
     1141 gccagccggc ccagcccagg ccggcgactc acccccgcca gccagaggcc cagcagtaaa
     1201 gccaccccgg cgtggagtaa taaacctgtc aggtgatagg ggaaggggtt gtcgccccac
     1261 agccgccagc ccaggcccca tgtcaggatc gtgctgatgg ggcgataaaa catccagttg
     1321 gggtcgggca gcaagatgcg cggattcaag ccccggttgc gcccggtgat cagcgtgaga
     1381 aagtcgtcgg acaggaagcc gatgcgcaac accgccaggt aggccagcac cgccagcagc
     1441 ggcaccaccg cccaggccag cgcgggagtt gcccggcgat acgccgtggc cacattggac
     1501 ccggcggcgc tcagccggtg cgaggcggag gccggtggat tactcagctc cgccccgctc
     1561 tgcgcatcat gccggggcgc tacaagcatg gcaggcaatc aggctctagt ggtcggagtc
     1621 gcggcgcgag acgagtacgc ccatccgccc gcccatccaa ccgaaccagg cccccaggcc
     1681 cagcgtggtc acgcggaaga acagcgcgtc gaggatggta ggttgcgcgg cgtccgccgg
     1741 gagatcgacc agttggacca gcgcgtcggc gggcacgatg atctcggtca cggtgttgat
     1801 cagggccacg atcaccccgg cggtaatccc ggcgaccagg ccgctgacgc gctcgccggt
     1861 ggcccgcgcg gccagcaggc cggcgacgaa gggcagcgcc gcttccagca tcagcgacag
     1921 acattcggtg atctggtaat cggccaggtt ctgcgaccgc agcgaggtca ggtagccgag
     1981 cgtttcaaac aacgccaggg cgccgccgaa cagggcgccc cacttgagcg cgctatagag
     2041 ttttggtgtc atacatgccg ggccgccgcc cccgtttccc ggccatcggc cagggggcgc
     2101 ggccctcagc tccttaaggg caaagttcga tgcaacggcc cggaccaggc gggccgcacc
     2161 cattataaca cccgcgcctg ttcagcagca atcaacagcg acggcgcggc ggcgcacctt
     2221 gacagggggc cggttctcct atatcatata agcgctcgct tatggattcg ccgggcgagc
     2281 cgattgagac gtagtgcacc acggaaggac agctcgccgg tattatgcag aacggcctct
     2341 cggagtccgc cccccgaacc caactggaat cgtggggcgc ggccctggcg ctcaaaccct
     2401 atttgcacgc catcgcggac cccacccgcc tcagcatgct gcacgagctg gcccaggcag
     2461 gcgaacacca ggtgctggaa ctggcccggc tgctcggcct cagccaaccc ctcaccagtt
     2521 ggcacctgcg tatcctcaag caagcgggcc tcgtgtccac acggcgcctg ggccgccagg
     2581 tcctctgccg cctcaaccgg gagcgtctca gcgagttcaa agcccaattc gacgcgctta
     2641 tcaacctgta gccgccggat gccgggtcgc ggctcagcac cgaccagatc aagcaagtag
     2701 catgaagtaa ggagtaatga agcatatggg taaagttcgc gtggctatca tcggcgtggg
     2761 caactgcgct tcgtcgctgg tccagggcgt gcatttctat cgcaatgccg aagacgacga
     2821 gttcatcccc ggcctgatgc acgtcaacct gggcggctat cacgtgggcg acgtggagtt
     2881 ctctgccgcc ttcgatgtgg acagccgcaa ggttggtacg gatctctccg aggccatctt
     2941 cgcggagc
//
LOCUS       DASUYC010001094         2999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1342262, whole genome shotgun sequence.
ACCESSION   DASUYC010001094 DASUYC010000000
VERSION     DASUYC010001094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1342262"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VF276_17825"
     CDS             <1..260
                     /locus_tag="VF276_17825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX6608763.1"
                     /translation="KVPGYSTAAKTGTAQIPGPNGAYDPNGTIASVIGFAPAHDPKFV
                     VLIRLDRPTSSQWGGETASPAFGRLAAQLFQYWKIPPDQKQ"
     gene            377..1777
                     /gene="murF"
                     /locus_tag="VF276_17830"
     CDS             377..1777
                     /gene="murF"
                     /locus_tag="VF276_17830"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEX6608764.1"
                     /translation="MYTLNDILSGTGGTLDPQYPGPAGLVLREVVIDSRTAGPGTLFV
                     ALPGQVYDGHEFVVDAVGHGAQAALVRADWQPPGPLGEGVAVIHVPDTLRGLQALATW
                     WRSRFPALKAIGITGSIGKTSTKEYTAAVLATCMQVFRSEGNYNSETGLPLSVLRLQP
                     EHEVAVFEMGGGSRMGELTDLAAIAQPQVGVITNVSHSHLASMGTLENLAYHKGELVR
                     ALPPAPVGVAVLNYDDERVRAMAATTPARVITYGLDPAADLWANEIESQGLGGVQFTL
                     HRAEESMRLRLPLLGVHSVHTALRAAAACEAVGMNWKDIVVGLQTGEAQQLRLLVAAG
                     INGTTIIDDSYNANPASTLAALNLLMEMPHPRLAVLGDMLELGDYTEEGHIKVARRAI
                     EIVDRLVVVGQLGRLIGEEALRCGMAPERIFFAPGNAQAIDYLRRVLAPGTYVLVKGS
                     HGMHMEEIVEGLRAPA"
     gene            1774..2847
                     /gene="mraY"
                     /locus_tag="VF276_17835"
     CDS             1774..2847
                     /gene="mraY"
                     /locus_tag="VF276_17835"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX6608765.1"
                     /translation="MMQHLFDFLLSIGAPTGTPLLDMPVAQITPLAGFVMIRALALGG
                     LAFLIGLVLGRPIINQLRARRIGKQIRIEGPQSHLVKMGTPTMGGIIFLVTVVICIVL
                     FMDLPHFKSLLLPLGIIVSCGLLGAVDDRLSIVGGKKREGMTARFKMLWLILIAAAAA
                     WVLHYPLGLTSIYIPFSGLRRIDIGMWYIPVAIIGIVGMANAVNLSDGLDTQAAGMAA
                     MSFVAYGIIAYLQRQEPVVYLCFATVGALLAFLWYNAHPAQVFMGDTGSLALGALLAV
                     CAFMTGQWLVLPVVGIMFVAISASSMLQVIYFKLTGGRRLFKMAPLHHHFELLGWSET
                     QVAMRFWLISMLGGMLGVALALA"
BASE COUNT          464 a          914 c         1087 g          534 t
ORIGIN      
        1 cgaaggtgcc gggctattcc acggcagcca agaccggcac ggcgcagatc cccggcccca
       61 acggggccta cgacccgaac gggacgattg cctcggtgat cgggtttgcg ccggcccacg
      121 acccgaagtt tgtggtgctg atccggctgg accggcccac gtcgagccaa tggggcggtg
      181 agacggcgtc cccggccttt ggccggctgg cggcgcagtt gttccagtat tggaagatcc
      241 cgcctgacca gaagcagtag gccgggcgcc aggccggttg caaaccggcc cctacacccg
      301 gacagcaggc cggttgcaaa ccggccccta caactagaag cgtagtgacg gacggtgcgc
      361 ccgtcgataa ggatttatgt acacactgaa cgacatttta agcggcacag gaggcacgct
      421 ggacccgcag tatccgggac cggcgggcct cgtgttgcgc gaagtggtaa tcgattcgcg
      481 cacggcgggg ccgggcacgc tgtttgtggc cctgcccggc caggtctacg acgggcacga
      541 attcgtggtc gacgccgtgg gccacggggc gcaggccgca ttggtgcgcg ccgactggca
      601 gccgcccggc ccgctgggcg agggcgtggc cgtgatccac gtgcccgaca cgctgcgcgg
      661 cttgcaggcg cttgccacct ggtggcgtag ccggttcccc gcgctcaagg ctatcggcat
      721 taccggcagc atcggcaaga cgagcaccaa ggagtatacc gccgccgtgc tcgcgacctg
      781 tatgcaggtc ttccgcagcg agggcaacta caacagcgag accgggctgc cgctgagcgt
      841 gctgcgcttg cagccggaac acgaggtggc cgtcttcgag atgggcggcg gcagccgcat
      901 gggcgagtta accgacctgg cggcgattgc ccagccgcag gtcggcgtga tcaccaacgt
      961 gagccactcg cacctggcga gcatgggcac gctggagaac ctggcctacc acaagggcga
     1021 gttggtacgc gcgctgccgc ccgcgccggt gggcgtggcc gtgctcaact acgacgacga
     1081 gcgcgtacgc gccatggccg ccaccacgcc ggcgcgggtc attacctatg gcctggaccc
     1141 ggcggcggat ctgtgggcca acgagatcga gagccagggc ctgggcggcg tgcagttcac
     1201 gctccaccgc gccgaggaat cgatgcgctt gcgcctgccg ctgctgggcg tccatagcgt
     1261 gcataccgcg ttgcgggcgg cggcggcctg cgaggcggtg ggcatgaact ggaaggatat
     1321 cgtggtcggc ctgcaaacgg gcgaggcgca gcagttgcgc ctgctggtgg ccgcgggcat
     1381 caacggcacg acgatcatcg acgacagcta taacgccaac ccggcctcga cgctggccgc
     1441 gctcaacctg ctgatggaga tgccgcatcc gcggctggcc gtgctcggcg atatgctgga
     1501 gctgggtgac tataccgaag aggggcacat caaggtggcg cgccgggcca tcgagatcgt
     1561 cgaccggctg gttgtggttg ggcaactggg gcgcctgatc ggcgaggagg cgctgcgctg
     1621 cggcatggcg ccggagcgga tcttcttcgc gccgggcaat gcccaggcca tcgattacct
     1681 gcgccgcgtg ctggcgcccg gcacctatgt gctggtcaaa ggctcgcacg gcatgcacat
     1741 ggaagagatc gtcgaggggt tgcgagcacc ggcatgatgc agcacctgtt cgactttctg
     1801 ctgagcatcg gggcccccac cgggacgccg ctgctcgata tgccggtcgc ccagatcacc
     1861 ccgctcgcgg gcttcgtgat gatccgggcc ctggcgctgg ggggcctggc cttcttgatc
     1921 ggcctggtgc tgggccgccc gatcatcaac cagttgcggg cgcggcgcat cggcaagcag
     1981 atccgcatcg aggggccgca atcgcacctg gtgaagatgg gcacgccgac gatgggcggg
     2041 attatcttcc tggtgacagt cgtcatctgt atcgtgttgt ttatggacct gccgcatttc
     2101 aagtcgctgc tgctgccgct gggcatcatc gtctcgtgcg ggctgctggg cgcggtggac
     2161 gaccggctga gcatcgtcgg cggcaagaag cgcgagggca tgacggcgcg cttcaagatg
     2221 ctctggctga tcttgatcgc ggcggcggcg gcctgggtgc tgcattaccc gctggggctg
     2281 accagtattt acatcccgtt ttcggggttg cgccgcatcg acatcggcat gtggtatatc
     2341 ccggtggcga tcatcggtat cgtgggcatg gcgaacgcgg tcaacctgag cgacgggctg
     2401 gacacgcagg cggcgggcat ggcggccatg tcgttcgtgg cttacggcat catcgcctac
     2461 ttgcagcggc aggagccggt ggtctacctg tgctttgcca ccgtgggggc gctgctggcg
     2521 ttcctgtggt acaacgctca cccggcgcag gtgtttatgg gcgacacggg gtcgctggcg
     2581 ttgggggcgc tgctggccgt ctgtgccttc atgaccgggc agtggctggt gctgcccgtg
     2641 gtcggcatca tgttcgtcgc catctcggcg tcgtccatgt tgcaggtgat ctatttcaag
     2701 ctgaccgggg gccggcgcct gttcaagatg gcgccgctgc accaccactt cgagttgctc
     2761 ggctggtccg aaacccaggt ggcgatgcgc ttctggttga tcagcatgct gggcggtatg
     2821 ctcggcgtgg ccctggcact ggcgtagtgc tgagtgctga gtgctgagtg ttgcgagtgg
     2881 tcggtggtca gtggtcagta ggacgagtag tgagggacct gacgaggttg cgtgtagggg
     2941 ccggtttgaa accggcccgg tggggcaaga tgtggtgtag gggccggttt caaaccggc
//
LOCUS       DASUYC010001095         2880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1387010, whole genome shotgun sequence.
ACCESSION   DASUYC010001095 DASUYC010000000
VERSION     DASUYC010001095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1387010"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VF276_17840"
     CDS             <1..343
                     /locus_tag="VF276_17840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608766.1"
                     /translation="VERRYRKPPAGATPPSSGAVPQPPAGTTAPPSGTLPRDTGTGTS
                     GNYTGPITSAPADTGGPGVRTLQDVRSAPVPTRGAKDARRSADGGATESPSDAPYAGP
                     HREPGGYGEVQ"
     gene            complement(425..2581)
                     /locus_tag="VF276_17845"
     CDS             complement(425..2581)
                     /locus_tag="VF276_17845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608767.1"
                     /translation="MNRVTRHRSHPIWHLLTVIPVLAMIAGLGSWTAVAAPLAAPATS
                     GCQLNSPNGAVQHVIYIQFDNTHFTRDNPNVPSDLEQMPHLLNFIENNGVMLANHHTP
                     LISHTATDILTSLTGVYGDRHGVPVSNSFRYFLPNGTTHTGVSFAYWTAPLFDPANPN
                     PPIDTSYNMLTADGKNAPAPWVPYTRAGCNVGAVATANTILENTAIDIPTVFGANSPE
                     AQEVIANPGQAFADFVGIGIHCAQGDALCASANHGRPDLLPDEPGGYSGYQGLFGHKY
                     TVPQISPNGPLTDLNGNVIQDPAGHVGFPGFDGMAATVSLAYVAAMQEHGVPVTYAYI
                     SDAHDAHPTGPAYGPGSAGYVAALARYDDAFDRFFQRLAADGINAGNTLFVFTADEND
                     HFAGGPPSPANCDGVHTPCTYSLIGEVNTNMAGLLATEQGITTPFTVHSDDAPTIYLT
                     GNPARDALVTRNFGRAVGALTAVNPYTNQQENLTLGLADPVEMKLLHMITADPARTPT
                     LTMFARPDYFLFAGAPNCNSPCVAVNPAFAWNHGDVTPDITVTWLGMVGPGVRHEGVN
                     HSVWSDHTDIRPTMLMLLGLHDDYLHDGRAITEPLYAWALPPSLLAHRETLLRLGAVY
                     KQINAAVGRLGLDSLQISTQALESGSPADDSTYTTLENQLATITARRNALAAQMQALL
                     ESATFDNQPADEQQAKALIAQGQALLDDVAALANGH"
BASE COUNT          424 a          935 c         1062 g          459 t
ORIGIN      
        1 agtcgagcgg cgctaccgca aaccgcccgc gggcgcgacg ccgccatcca gcggcgccgt
       61 gccgcagccg cccgcgggta cgaccgcgcc gccgagcggc acgctgccgc gtgatacagg
      121 caccggaacc tccggcaact acaccggtcc catcaccagc gcgcccgcgg acaccggcgg
      181 gcccggtgtg cgtaccttgc aggacgtgcg ctccgcgccc gtgcccacgc gcggcgccaa
      241 agacgcccgc cgctccgcgg acggtggcgc caccgagtcg cccagcgatg cgccctatgc
      301 cggcccgcac cgcgaaccgg gcggctacgg cgaagtccag taggcccggc gcggcgccgc
      361 gaagccaaca gggcgaccca tgcgggtcgc cctgtcgtct atccaccggg gcgggcgccc
      421 cggcttagtg tccgttcgcc agcgccgcca cgtcgtcgag cagcgcctgg ccctgcgcga
      481 tcagggcttt ggcctgctgc tcatcggcgg gctggttgtc gaacgtggcc gactccaaca
      541 gcgcctgcat ctgcgccgcc agcgcgttgc gccgggcggt gatggtggcg agctggttct
      601 ccagggtggt ataggtgctg tcgtcggcgg gactgccgct ttccagcgcc tgggtcgaga
      661 tctgcaagct atccaggcca agccggccca ccgcggcgtt gatctgcttg tagacggcgc
      721 ccaaccgcag cagcgtctcg cggtgcgcga gtagcgacgg cggcagcgcc caggcataga
      781 gcggctcggt gatcgcgcgt ccatcgtgca ggtagtcatc gtgcagcccc agcaacatca
      841 gcattgtcgg gcggatgtcg gtgtggtccg accagacgct gtgattgacg ccctcgtggc
      901 gcacccccgg cccgaccatg ccgagccagg tcacggtgat gtcgggcgtc acgtcgccgt
      961 ggttccaggc gaaggccggg tttaccgcca cgcaggggct attacagttg ggcgccccgg
     1021 caaacaggaa gtagtcgggc cgcgcgaaca tcgtcagcgt cggtgtgcgc gccgggtccg
     1081 ccgtgatcat gtgcaacaac ttcatctcca ccgggtcggc caggcccagc gtcagattct
     1141 cttgctggtt ggtgtacggg ttcacggcgg tcagggcgcc cacagcccgg ccgaagttgc
     1201 gggtcacgag cgcgtcgcgc gccggattgc cggtgaggta gattgtgggc gcgtcgtccg
     1261 agtggaccgt gaagggcgtg gtgatgccct gctcggtcgc cagcaggccg gccatgttcg
     1321 tgttgacctc gccgatcagg ctgtaggtgc agggcgtatg cacgccgtcg cagttggccg
     1381 ggctgggcgg gccaccggca aagtggtcgt tctcgtcggc ggtaaatacg aagagcgtgt
     1441 tgccggcgtt gatgccgtcg gcggcgaggc gctggaagaa gcggtcgaag gcgtcgtcat
     1501 agcgggcgag ggccgccacg tagcccgccg agccggggcc gtaggccggg ccggtggggt
     1561 gggcgtcgtg ggcgtcggag atataagcgt aggtcaccgg gacaccgtgc tcctgcatcg
     1621 ccgccacgta ggccagcgag accgtcgccg ccatgccgtc gaagccgggg aagccgacgt
     1681 ggcccgccgg gtcctggatc acgttgccgt tcaagtcggt cagcggcccg ttggggctga
     1741 tctgcgggac ggtgtacttg tggccgaaga ggccctggta gccgctgtag ccgcccggct
     1801 catcgggcag caggtcgggc cggccatggt tggcgctcgc gcagagcgca tcgccctgcg
     1861 cgcagtggat gccgatgccc acgaagtcgg cgaacgcctg gcccggattg gcgatcactt
     1921 cctgggcttc gggcgagttg gcgccgaaca cggtcgggat gtcgatggcc gtgttttcga
     1981 ggatggtgtt cgccgtcgcc acggcgccga cgttgcagcc ggcgcgggtg tagggcaccc
     2041 acggggcggg cgcgttcttg ccgtctgccg tcagcatgtt atagctcgtg tcgatggggg
     2101 ggttcgggtt ggccgggtcg aacagcgggg ccgtccagta ggcgaaggac acaccggtgt
     2161 gcgtggtgcc gttgggcagg aaatagcgga agctgttgct gaccggcacg ccgtggcggt
     2221 cgccgtatac ccccgttagc gaggtcagga tgtcggtcgc cgtgtgcgag atcagcggcg
     2281 tgtggtggtt ggcgagcatg acaccgttgt tctcgatgaa gttgagcagg tggggcatct
     2341 gctccaggtc cgacgggaca ttgggattat cgcgggtgaa gtgggtattg tcgaactgaa
     2401 tataaatcac gtgctggacc gcgccgttcg gggagttcaa ctggcagccg ctggtggcag
     2461 gtgccgccag gggcgcggcc acggcggtcc aactgcccag cccggcgatc atcgccagga
     2521 ccgggatcac ggtcagcagg tgccaaatgg ggtgagatcg atgtcgggta acgcgattca
     2581 cggtcgctcc tcctcaaggc cgctaacagc cctccggcag ggggcacctg ccgccgcgcc
     2641 gcccaaccgg gcaggcgggg acacgccgtt agcgggaacc gtaactggtg gccgcgtgat
     2701 acctgaccct ccatgcgcaa tctacccaga ccgttctggg tacccgcacg ctcctcctcg
     2761 cgcgtgcggc aaacggcggg atccgcaacc gcctcgcaat gccgggtgcc ggggtggcga
     2821 accgggcggc gccgggcgcg ccgcagatcc gcaagcaggc tggacagcac caccccgtat
//
LOCUS       DASUYC010001096         8647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_2036928, whole genome shotgun sequence.
ACCESSION   DASUYC010001096 DASUYC010000000
VERSION     DASUYC010001096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 8647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8647
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2036928"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1180)
                     /locus_tag="VF276_17850"
     CDS             complement(<1..1180)
                     /locus_tag="VF276_17850"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6608768.1"
                     /translation="MAGNKAAFDAAMKRAQDYAFENAWDKALREYQRALDEFPDDANA
                     QTNMAQALFRLQQWPAALQAYNNLLRVRPSDPFVLNRIAECQISLQQPADAERTYMPV
                     ATVYGAQNQVRDALAALRTVLGLNSGNRLARERMAEIYRNMGERNLAIQEYLTLSRQA
                     LESQPEDLDLAVRHAETAFGLDNSHPEARDWLYRLRRRQLEATGTQFDEKTLRIVSGP
                     GQAAQRDLQQMYDLAMQYQEKNQFAAARVQLEAAQAAGLDTAALHYSLGLIYQQERQF
                     KQAVEQLQKAAADPEYAMSSQYALGECYRTLGQKAEATRAYEAAVSLVDLQHVGKNEV
                     NDLIELYQAAADAHLAQGNTSRAASLYSTLATFLQARRWRSGFTDQLISRADELNDQG
                     I"
     gene            complement(1586..2425)
                     /locus_tag="VF276_17855"
     CDS             complement(1586..2425)
                     /locus_tag="VF276_17855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6608769.1"
                     /translation="MERVGKYLAELSIPVNISPENTPISSGASEVCPNCHGAGYLRLE
                     VPLGHPNFGRLFPCQCKLRELADKTHSGMERLSNLDAFAAKTFATFDPASPGVREAFM
                     RCVEYAQNPTGWLLLLGSYGCGKTHLAAAIANAVLERGLQTYFAIAPDLLDHLRAAYA
                     PNSEGSYDDRFEAVRTVPLLIIDDLGTENTTPWAREKLYQIFNHRYNYRMPTVVTSNV
                     DLDALDPRIASRLCDQQLCGHIFIDAPDYRQRAAPRRPRDTGSNGGNAGRSSAARRPS
                     RGQ"
     gene            complement(2337..3152)
                     /locus_tag="VF276_17860"
     CDS             complement(2337..3152)
                     /locus_tag="VF276_17860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012984226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEX6608770.1"
                     /translation="MFNGFQSEEKRISLPESFFTSVLPQITNLAELKVTLHVFYRLAA
                     QVGQPRLLSYPELAEDRALAAALTAGRNPRPAEEWLREGLELAVARGTLLQLIVQPEG
                     GAADDEFWYLVNTPFNKRWLAQMCGEEAGAETGGLASAQWLALLRAERDAALGRAPDS
                     VAEGAPDAERVRLVQRRANIFELYEQNIGVITPLLADQLTEAERLYPAAWIEEAFTEA
                     VNYNKRSWSYVRRILENWAAKGRDDGTRGQVSGGTLDPGKYLTGKYAHLFGRK"
     gene            complement(3216..5876)
                     /locus_tag="VF276_17865"
     CDS             complement(3216..5876)
                     /locus_tag="VF276_17865"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015717917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX6608771.1"
                     /translation="MAVSIPNNTGLYSSGDGPLERPVPYDLQAEEAVLGALLLDRDAV
                     IKVATFLQPADFYREANGWIYDCVLDLYHRREPPDLVTLSSELDRRGRLEPVGGWAYL
                     AALMHGVPTAVHVEYYARIVERNAVLRRLISVGGQIAGLGYEEGLDLPVVLDKAESLL
                     FGVTQRQSNADFVSIKRVLEEYFDRLDIVQHQRGNVVGVPTGFHDLDELTGGLQKSDL
                     VIVAARPSVGKSSFGLSIAYNAAVQHGKSVGVFSLEMSAEQLVQRLLSICSGVETQRL
                     RLGYMSDTEWQSLSDAFGTMSDVPIYFDDSAGLSVTEVRSRARRLQAEAGCDLLIIDY
                     LQLMSGRKSDNRVQEVSEITRGLKQLARELNVPVIAMSQLSRAVESRTSHVPMLSDLR
                     ESGCLSGDTPVYLPDQGIYRPIEQLVGQSGFRVLALNTQTWKLEPCAVSNAFATGHKP
                     VYRLTTRLGRSICATANHKFLTIHGWKRLDELAPGGRIALPRQLPGPETATMTGDELA
                     LLGHLIGDGCTLPRRSIQYTTRELGLAETVAGLARNVFGSDVVPRIHAERTWYQVYLP
                     AREQVTHNKHNPIVTWLDSMSVFGLRSYEKRVPDRVFAQPAAGIARFLRHLWSTDGCV
                     HLSKTNSIGVYYASSSPQLAQDVQSLLLRLSINAVISRSPQPGKGRDQYHVAIMGKPD
                     IERFLTIVGGLGEHKIVHQAAIMSYLAERVTNTNRDVLPRDIWRLVAVPAMQAAGVTS
                     RQMQAGLGNAYCGTGLYKQNISRDRAARLAEVVRSDELTRLAQSEVYWDEIVTIEPDG
                     ERAVYDLTVDSLHNFVAGNIIVHNSIEQDADIVMFIYREELYNPETERKNIAEIHIAK
                     HRNGPTGVVPLFFKNSTTHFSDLDVYRQPS"
     gene            complement(5983..6825)
                     /gene="rplI"
                     /locus_tag="VF276_17870"
     CDS             complement(5983..6825)
                     /gene="rplI"
                     /locus_tag="VF276_17870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX6608772.1"
                     /translation="MKVILTETVEKLGQPGDVKEVANGYARNFLFPRNLAIPATKGAL
                     QEAANRRASTEKRAAQQASQAQAAVDKLNGLTVLLYARTGEQNRLYGSITPSDVAQAL
                     QVQHGETVDRRRIKIDTPIHRTGNFTATVSAGNNLSAKLNLVVEPETSRTGALTSVAA
                     QAPAPPPAPAEAAPAAGTAPEPAAPAETPVEAAPAAEAPVAEVTPATEAPVAEAAPAT
                     EAPVAKAAPATEAPEAETTPATDTAATPSTAPEAVTFAPTDVAPVAEAASAAAGEPEA
                     APEV"
     gene            7311..>8647
                     /locus_tag="VF276_17875"
     CDS             7311..>8647
                     /locus_tag="VF276_17875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6608773.1"
                     /translation="MPDKVDTAVVAERVAPSIPRAARAGALAADAPPVGAPVPARGAK
                     LSAGFRALRHRNYRLFFFGQLISLIGTWMQTVAQAWLVLQLAGKDADLMLGITSALQF
                     APVLLLSVVGGALADRLPKRNVLVVTQSVMMVLAFVLAVLAFTHVVQIWHVMVLAALL
                     GTTNAIDMPTRQAFVVEMVGKEDLMNSIALNSSVFNAARLVGPAIAGVLISLVGVVGC
                     FFLNGVSYLGVITGLLLMRAPFFTNTSKEAPPQGWGTKGGFLANLGGGFRYIRHTPAV
                     FALVALVGAVGTFGMNFTVWMPVMARDILHVGADGYGLMMSTMGAGSLVSGLALAYGA
                     KQARRRVVIVAAFGFGLTLLAFAASSWFPLSLALLVAMGAAMVAFSATANTTVQMTVP
                     DELRGRVMAVYMMVFAGTTPIGSLLAGTLAHATSTPISMAAGGAVSLLGVLGT"
BASE COUNT         1426 a         2768 c         2842 g         1611 t
ORIGIN      
        1 tgatgccctg gtcgttgagt tcgtcggcgc gtgagatcaa ctggtcggtg aagccgctgc
       61 gccagcggcg cgcctgcagg aacgtcgcca gcgtcgagta gaggctggcc gcccgcgagg
      121 tgttgccctg ggccaggtgg gcatccgccg ccgcctgata gagctcaatc aggtcgttga
      181 cctcgttctt gccgacatgc tgcagatcga ccaggctgac ggcggcctcg taggcgcggg
      241 tagcctcagc cttctggccc agcgtgcggt aacattcgcc cagcgcgtac tggctcgaca
      301 tcgcatattc gggatcggcg gcggccttct gcaattgctc gacggcctgc ttaaactggc
      361 gctcttgctg gtagatcagg cccaggctgt aatgcagcgc cgccgtgtcg aggccggccg
      421 cctgcgcggc ttccaactgc acgcgggccg ccgcaaactg gttcttctcc tggtactgca
      481 tcgccaggtc gtacatctgc tgcaggtcgc gctgcgcggc ctggcccggc ccactgacga
      541 tgcgcaacgt cttctcgtca aactgggtgc cggtggcttc gagctggcgg cggcgcaggc
      601 gatagagcca gtcgcgcgct tcggggtggc tattgtcgag gccgaaggcg gtttcggcgt
      661 ggcgcacggc caggtcgagg tcctcgggct ggctttccag ggcctggcgc gaaagggtca
      721 ggtattcctg gatggcgagg ttgcgctcgc ccatgttgcg atagatctcg gccatgcgct
      781 cgcgcgccag ccggttgccc gaattcagcc ccagcacggt acgcagcgcg gccagggcgt
      841 cgcggacttg gttctgcgcg ccatagaccg tcgccaccgg catataggtg cgctcggcgt
      901 cggcgggctg ctgcaagctg atctggcact cggcgatgcg gttcagcacg aacgggtcgc
      961 tggggcgcac ccgcagcagg ttgttatatg cttgcagcgc ggcgggccac tgctgcaagc
     1021 ggaacagcgc ctgggccatg ttggtctgcg cgttcgcgtc gtcggggaac tcgtcgaggg
     1081 cacgctggta ctcgcgcaag gccttgtccc aggcgttctc aaaagcgtaa tcctgtgccc
     1141 gcttcatcgc ggcatcaaac gccgctttat tgcccgccac tgcgtgcctc ctctacctgg
     1201 ggggacgttt tagacagatc gtgaggccgg gcgcgctgat cgccgccggt ggttttagta
     1261 taccataaga cctgacaggc tgggtagggg aaatcgccat ctggccgggg cgggaatcgg
     1321 ttgtggataa ttcgtggata aagtggtgga taaacaggat tgacagggcg ggcgcccggt
     1381 gctatgatga gggcacttca gtaaattgtt cttcggaaca gagtactgga gagcaaggcc
     1441 ggaggatccg caaggattgt ccggccttcg cgttttcccg gcgaaccgct gaggcggcag
     1501 gccggtttca aacccgcccc tacagcggac ggtgaggcgg gccggtttca aacccgcccc
     1561 tacaaatcag aattacgggc gccctctact gcccccggct ggggcgtcgc gccgccgacg
     1621 agcgtccggc gttcccgccg ttggaacccg tatcgcgcgg gcggcggggc gccgcgcgct
     1681 ggcggtagtc gggcgcgtcg atgaagatgt ggccgcacaa ctgctggtcg cacagccgcg
     1741 aggcgatacg cgggtcgagc gcgtcgaggt cgacgttgga cgtgaccacg gtgggcatgc
     1801 ggtagttgta gcggtggttg aagatctggt agagcttttc gcgcgcccag ggcgtggtgt
     1861 tctcggtgcc caggtcgtcg atgatcagca gcggcaccgt gcgcacggcc tcgaagcggt
     1921 cgtcatacga cccctcgctg ttcggggcat aggcggcgcg caggtggtcg agcaggtcgg
     1981 gcgcgatggc gaaataggtc tgcaggccgc gctccaggac ggcgttggcg atagcggcgg
     2041 ccaggtgggt cttgccgcag ccgtacgagc cgagcagcag cagccagccg gtggggttct
     2101 gggcgtattc gacgcagcgc atgaaggcct cgcgcacgcc gggcgacgcc gggtcgaatg
     2161 tcgcgaacgt cttggcggcg aaagcgtcga ggttgctcag ccgttccatg ccgctgtgtg
     2221 tcttgtccgc cagttcgcgc agcttgcact ggcatgggaa cagccgcccg aagttcgggt
     2281 ggcccagcgg cacttccagg cgcaggtagc ccgccccgtg gcagttggga cagacctcac
     2341 ttgcgcccga agagatgggc gtattttccg gtgagatatt taccgggatc gagagttccg
     2401 ccagatactt gcccacgcgt tccatcgtct cttcccttcg ccgcccagtt ctccagaatc
     2461 cggcgcacat acgaccaact gcgcttattg tagttgacgg cctcggtgaa agcctcttcg
     2521 atccaggccg ccgggtacag gcgctcggct tcggtgagct ggtcggccag cagcggcgtg
     2581 atcacgccga tgttctgctc atacagctcg aagatattgg cccgccgttg caccaggcgc
     2641 acgcgctcgg catccggcgc gccctcggcc acgctgtccg gcgcgcgccc caacgcggcg
     2701 tcgcgctcgg cgcgcaagag agccagccac tgcgccgagg ccaggccgcc ggtttccgcg
     2761 cccgcttcct cgccgcacat ctgggccagc cagcgtttgt tgaacggcgt attgaccagg
     2821 taccagaact cgtcgtccgc cgcgcccccc tccggctgga cgatcagttg cagcaaggtg
     2881 ccgcgggcca cggccagttc cagcccctcg cgcagccatt cttcggcggg gcgcgggttg
     2941 cggcccgccg tcagcgccgc ggccagcgcg cgatcctccg ccagctccgg gtagctcagc
     3001 agccggggct ggcccacctg ggccgccagc cggtagaaca catgcagtgt caccttcagc
     3061 tcagccagat tcgtgatctg cgggaggaca gacgtgaaga agctctccgg cagcgagata
     3121 cgcttctcct cgctctggaa cccgttaaac ataagaggcc cctcgactta ccgtcgcacg
     3181 tccatttcgg gggccggcgt cactccgggc cacgcttaac tgggttgccg gtacacatcc
     3241 agatcgctga aatgtgtagt tgaatttttg aagaagaggg gaacgacgcc cgtcgggccg
     3301 ttccggtgct tggcgatatg gatctcggcg atgttcttgc gctcggtctc cgggttgtag
     3361 agttcctcac gataaatgaa cattacgatg tcggcgtctt gctcgatgct gttgtgaacg
     3421 ataatattgc cggctacaaa attgtgcaac gagtccacag tcagatcgta aacggcacgt
     3481 tcaccgtcag gctcaattgt cacgatctca tcccagtata cttcactctg ggcaagccgg
     3541 gtcaactcat cactgcgcac aacctccgct aatcgtgcgg cccggtcgcg gctaatattc
     3601 tgcttgtaaa gacccgtgcc acagtaagca ttaccgaggc ccgcctgcat ctgccgggaa
     3661 gtcacgcccg cagcctgcat ggcgggtaca gccaccaggc gccatatgtc cctgggtagc
     3721 acgtcacgat tcgtgttggt aacccgctcc gccagatagc tcataatagc cgcctgatgt
     3781 acaattttgt gttcgccgag gcccccgact atcgtcagga aacgctcaat atcaggcttg
     3841 cccataattg ccacatggta ttgatcgcgt ccttttcctg gctgaggaga acgcgagatc
     3901 actgcattaa tactgagacg cagcaacaaa gattggacat cctgcgccag ttgcggactg
     3961 ctggacgcat agtacacgcc tatagagttc gtcttgctca ggtggacgca gccgtccgtc
     4021 gaccataaat ggcgcagaaa gcgggcgata ccggcagccg gttgggcgaa aacccgatcc
     4081 ggtacacgct tctcataaga gcggaggccg aagacgctca tgctgtccaa ccatgtgaca
     4141 atcgggttgt gcttgttgtg tgtaacctgt tcgcgagccg gcaaatagac ttgataccag
     4201 gtccgttccg catgaattcg cggcacaaca tcggacccaa acacatttcg tgccaagccc
     4261 gcaacagtct cggccaagcc caactcccgc gtggtgtact ggatagatcg acgcggcaaa
     4321 gtgcagccat ccccgatgag atgccccagg agcgccagct cgtcgccggt catggtcgct
     4381 gtctcagggc cgggtaattg gcgaggtaaa gcaatgcgcc cgccaggtgc cagttcatcc
     4441 aggcgtttcc agccatggat cgtcaggaac ttatggtttg ccgtcgcgca tatcgaacga
     4501 cccaggcgcg tggtgaggcg gtacaccggc ttatggcccg tcgcgaaagc attgctcacg
     4561 gcgcaaggct caagcttcca ggtctgtgta ttcaaagcaa ggacccggaa cccggactgg
     4621 ccgaccagtt gttcaatagg tcgataaata ccctgatccg gcaggtaaac gggtgtatcg
     4681 ccgctcaaac accccgactc gcgcaagtcg ctgagcatcg gcacgtgcga cgtgcgactt
     4741 tccaccgcac gggaaagctg cgacatggcg atcaccggca cgttgagttc gcgcgccagt
     4801 tgcttcaggc cgcgcgtgat ctccgagacc tcctgcacgc ggttgtcgct tttgcgcccg
     4861 ctcatcagtt gcaggtagtc gataatcagc agatcgcatc ccgcttcggc ttgcaggcgg
     4921 cgcgcacggc tgcggacctc cgtcacgctc aggcccgcgc tgtcgtcgaa atagatcggc
     4981 acgtcggaca tggtcccgaa ggcatcgctc aacgactgcc actcggtgtc gctcatgtag
     5041 cccaggcgca ggcgctgcgt ttcgaccccg ctgcaaatag atagcaagcg ctggaccaac
     5101 tgttccgccg acatttccag gctgaacacg cccacgctct tgccgtgctg cacggcggcg
     5161 ttataggcga tgctgaggcc gaagctgctc ttgccgaccg aggggcgcgc cgcgacaatc
     5221 accaggtcgg acttttgcag accgccggtc agttcgtcga gatcgtggaa gccggtgggc
     5281 acgccgacga cgttgccccg ctggtgctgg acgatgtcga gccggtcgaa atactcttcg
     5341 agcacgcgct tgatcgacac gaagtcggcg ttgctctggc gttgggtgac gccgaagagg
     5401 agcgactcgg ccttgtcgag cacgaccggc agatccaggc cctcttcgta gccgaggccg
     5461 gcgatctgcc cgccgacact gatcaggcgg cgcaggacgg cgttgcgttc gacgatgcgc
     5521 gcgtaatatt cgacgtggac ggcggtgggg acgccgtgca tgagcgcggc caggtaggcc
     5581 cagccgccca ccggttcgag ccgcccgcgc cggtcgagtt cgctggaaag cgtgaccagg
     5641 tcgggcggct cgcgccggtg gtagagatcc aggacgcaat cgtagatcca gccgttggcc
     5701 tcgcggtaga agtcggcagg ctggaggaac gtggcgacct tgatgacggc atcacgatcc
     5761 agcaagagag cgccgagcac ggcttcctcg gcttgcagat cgtaggggac agggcgctcc
     5821 agcggcccat cgccgctgga atagaggccg gtgttgttcg gtatcgaaac agccatagtc
     5881 agtggttagt acctatcatt gaggttagca gccgtactca acacgggcta ctaaccgctg
     5941 acacacgccg gtcgcccggc gcctcacgcg cccccgcggg cgctagactt cgggcgccgc
     6001 ttccggttcc ccggcggcgg cagacgcggc ctcggccacc ggcgccacat cggtgggggc
     6061 aaaggtcacg gcttccggcg cggtggacgg cgtagcagcc gtgtccgtcg ccggagtggt
     6121 ctcggcctcg ggcgcctcag tcgccggggc agctttcgct acgggtgcct cagtcgccgg
     6181 ggcggcttcg gccacaggtg cctcagtcgc gggagtgacc tcggccacgg gcgcttccgc
     6241 cgccggggcg gcttccacgg gcgtctccgc cggggccgcc ggctcgggcg cggtcccggc
     6301 ggccggggcc gcttccgcgg gggcgggcgg cggggccggg gcctgggccg ccacgctggt
     6361 caacgcgccg gtgcggctcg tctccggttc cacgaccagg ttcagcttgg cggacaggtt
     6421 attgcccgcc gacacggtcg cggtgaagtt gccggtgcgg tggatcggcg tatcgatctt
     6481 gatgcggcgg cggtccaccg tctcaccgtg ctgcacctgc agcgcctggg ccacatccga
     6541 cggcgtgatc gacccataga ggcgattctg ctcgccggtg cgggcgtaca gcaggacggt
     6601 cagcccgttc agcttatcga cggcggcctg ggcctggctg gcctgctgcg cggcgcgctt
     6661 ctcggtgctg gcgcggcggt tggccgcctc ttgcagggcg cccttcgtgg ccgggatggc
     6721 caggttgcgc gggaagagga agttgcgggc atacccgttc gcgacttcct tcacgtcgcc
     6781 cggctggccg agtttttcca cggtctcggt caaaatgact ttcatacgaa tctaactcct
     6841 atcgttctta tcgtatagag gcgattccca ccggctgcaa gcggtgcatc gccgataact
     6901 catctgttca aggcgccggc gcttgctaac aagccgcgca gcgcacccct accgcgggcg
     6961 cgcatcgggt caggccacct ctgaacagag gccattatag cacaaatgtg ccggaactcc
     7021 aaagcctgac ccgcgctgct gtccaccaat ttgtccacaa ccttttgaac agcccggctg
     7081 cctgactatc cacaactcat gcacaggtca cgcgggagac agtggataag ccggcgcctt
     7141 gctacagaaa accaatacct gtatattaac gttaacgtta aggtaagcat agtaatgaaa
     7201 ggcccgcgtc gcgtgcgccg ggcgcttccg gcccagtccc ggttgccggg acccgcggta
     7261 tacggccata taccgccgca acctgtacga gatcgaggaa gacggaacgt atgccagaca
     7321 aagtcgatac cgccgtggtg gccgagcgcg tggcgcccag cataccgcgg gcggcgcgcg
     7381 ccggcgcgct ggcggccgat gctccgccgg tcggcgcccc ggttcccgcg cgcggggcca
     7441 aactcagcgc gggcttccgc gcactccgcc atcgcaatta tcgcctcttt ttcttcgggc
     7501 aattgatctc gctgatcggc acctggatgc agacggtggc ccaggcgtgg ctggtgctgc
     7561 aactggccgg caaggacgcc gacctgatgc tgggcatcac cagcgcgctc caattcgcgc
     7621 ccgtgctgct gctgtcggtc gtcggcggcg cgctggccga ccggctgccc aagcgcaacg
     7681 tcctggtcgt cacccaatcg gtcatgatgg tcctggcctt cgtgctggcc gtgctcgcct
     7741 ttacccatgt cgtgcagatc tggcatgtca tggtgctggc ggccttgctg ggcaccacga
     7801 acgccatcga catgcccacc cgccaggcgt ttgtcgtgga gatggtcggc aaagaagacc
     7861 tgatgaactc gatcgcgctc aactcgtcgg tgttcaacgc ggcgcgcctg gttggcccgg
     7921 ccatcgcggg cgtgctgatc agcctggtcg gcgtggtcgg ctgcttcttc ctgaacggcg
     7981 tcagctacct gggcgtgatc accgggctgc tgctcatgcg cgcgcccttc ttcacgaaca
     8041 cgagcaaaga ggcccccccg cagggctggg gcaccaaagg cgggttcctg gcgaacctgg
     8101 gcggcggctt ccgctatatt cgccacacgc ccgccgtgtt cgcgctggtc gcgctggtcg
     8161 gcgcggtggg caccttcggc atgaacttca ccgtctggat gccggtcatg gcgcgcgata
     8221 ttctgcatgt aggcgccgac ggctatgggc tgatgatgtc gaccatgggc gcagggtcgc
     8281 tggtgtcggg gctggccctg gcctacggcg ccaagcaagc gcgccgccgg gtggtgatcg
     8341 tggccgcctt cggcttcggc ctgaccctgc tggccttcgc cgcctcgtcg tggttccccc
     8401 tctcgctggc cttgctggtg gcgatgggcg ccgcgatggt cgccttcagc gccacggcga
     8461 acacgacagt gcagatgacc gtgcccgacg aactgcgggg ccgggtcatg gcggtctata
     8521 tgatggtctt tgccgggacg accccgatcg gctcgctgct ggccggcacc ctggcccacg
     8581 ccaccagcac gccgatcagc atggccgcgg gaggcgcggt cagcctgctg ggcgtcctgg
     8641 gcacctg
//
LOCUS       DASUYC010001097         3048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1231000, whole genome shotgun sequence.
ACCESSION   DASUYC010001097 DASUYC010000000
VERSION     DASUYC010001097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1231000"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1230)
                     /locus_tag="VF276_17880"
     CDS             complement(<1..1230)
                     /locus_tag="VF276_17880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX6608774.1"
                     /translation="MTEGRIMRHEAKRAPIWLGFLLVGTLIKGIIWMLMIFPFDAPDE
                     PSHFSYITQLQSYRQLPVVVMPTPDRISIPPSTPQDAATQAMIHRFGFANFEGLSYES
                     SQPPLFYIAASLLTAPLGKNQLALLYGARLMAVLCGVGAVWALWATARTLWPEQPQIS
                     WIAPLVLTLHPQFTFITSTITNDACVFFLGTLLLWIWVKGLQSANEQDNGTVWRWILL
                     AGVVTGLGLLAKLTMIVTLPCTLLWLWWIAGARGGSMQGRLQRFMGGALIIGGAVILL
                     AGWWVLRNLLVYGEPTGTKALFELYRIYFRNEAPISLGGTQPDLKVLGQSIAVGIFSF
                     WALYGWGIVLLPPWEYAGPLLLAGLAVLGGARAIAAWRAGTPTDEQTQMRRRIAGLSV
                     LVLLVSLIDLILFNVNVA"
     gene            complement(1227..2147)
                     /locus_tag="VF276_17885"
     CDS             complement(1227..2147)
                     /locus_tag="VF276_17885"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX6608775.1"
                     /translation="MSNTPGPDAAVTTTDFPLPLFLRGKVRDTYELGNRLLLVATDRL
                     SAFDVVLPSGIPGRGEVLNRLSAFWFDRTRDLLPNHFITTDPAAYPFDTGGWPPDERA
                     KLAGRSMLVRRTRRIDIECVVRGYLSGSAWADYRQSGGREALGVALPAGLVESQELPE
                     PIFTPTTKAATGHDEPISFARLIMLVGTEMAEALRAASLQIYREAAAYARARGIIIAD
                     TKMEFGRLDDQLILIDELLTPDSSRFWDAARYAPGGPQPSFDKQYVRDWLTASGWDRE
                     SPAPVLPAEVIARTAAIYREAYTRLTDATL"
     gene            2232..2429
                     /locus_tag="VF276_17890"
     CDS             2232..2429
                     /locus_tag="VF276_17890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608776.1"
                     /translation="MASTPGSEEPPSSPREEPPAPADLMHNEREQLRIFGVPAIYIAL
                     IAFIILIAIMAWLGWLPTSVL"
     gene            complement(2400..2753)
                     /locus_tag="VF276_17895"
     CDS             complement(2400..2753)
                     /locus_tag="VF276_17895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608777.1"
                     /translation="MPDVRTRKILLIAGIIGLVFACVGLSQAVFARTVADFTGARPAL
                     IIAWLILMPLFAYTISLRGRARKSARQRALARKAALDAEAAQLRTADGVRAAGNAGAP
                     PAEPESLQHGSGEPA"
     gene            complement(2746..>3048)
                     /locus_tag="VF276_17900"
     CDS             complement(2746..>3048)
                     /locus_tag="VF276_17900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608778.1"
                     /translation="FAFQGAIGVGTFATLASLGLGLRGSTLGAAIFLVLALVSLGALA
                     YFGRIYLRLRRVRWQGELERPNAALQQLFDRARAEQAAAPAANEAPLPEGEDTDRA"
BASE COUNT          569 a          955 c         1016 g          508 t
ORIGIN      
        1 cgcgacattg acgttgaaga ggatcaaatc gataaggctc acgagcagca cgagtacgga
       61 tagcccggcg atcctgcgcc tcatctgggt ctgctcatct gtaggggtgc cggcccgcca
      121 ggcggcgatg gcccgcgctc ccccgaggac ggcgaggccc gcgagcagca atggtccggc
      181 gtactcccac ggagggagta gaacgatgcc ccagccatag agggcccaaa aggagaatat
      241 gcctacggcg atagactggc cgagcacctt gaggtccggc tgggttccac cgagactgat
      301 gggcgcctca ttccggaagt agatacgata cagctcgaag agtgcttttg tgccggttgg
      361 ctcaccgtag accagcaggt tacgcaaaac ccaccagcct gccaggagga taaccgcacc
      421 gcctatgatc agggcaccgc ccataaatcg ctgtaagcgg ccctgcatac tgcccccacg
      481 agcgccggcg atccaccaga gccagagcaa tgtgcagggc agtgtgacga tcatggtaag
      541 tttcgccaag agtccgagac ccgtcacaac acccgcgagc agtatccagc gccataccgt
      601 gccgttgtcc tgctcgttag ccgattgcag tccttttacc catatccaca gcagcaatgt
      661 gcccaaaaaa aacacgcatg catcgttggt aattgttgat gtgataaagg tgaactgtgg
      721 gtgcaaagtg agtaccagtg gggcaatcca actaatttgc ggttgctccg gccacagagt
      781 ccgtgccgtg gcccagagcg cccacactgc ccctacgccg caaagaactg ccatcaggcg
      841 ggcgccatat aggagcgcta gctggttttt tcccagcggg gcagtcagca aacttgctgc
      901 aatgtaaaat aacggtggtt gagaagactc gtaagacaag ccttcgaagt ttgcaaaacc
      961 gaaccggtgg atcatcgcct gagtcgccgc gtcttgtggg gtagagggcg ggatactaat
     1021 gcggtcaggc gtgggcatca cgaccaccgg aagttggcga tagctttgca attgggtgat
     1081 ataactgaag tgtgagggtt catcaggggc atcgaagggg aaaatcataa gcatccaaat
     1141 tatgcccttg atcaacgtgc ccactagcag gaaccccagc cagatagggg cgcgctttgc
     1201 ttcgtgccgc atgattctgc cttcggtcat agggtcgcgt cggtcagccg cgtataggct
     1261 tcccgataaa tagcagcggt ccgcgcgatc acctcggcgg gcagcacggg ggcgggcgac
     1321 tcgcggtccc agccgctggc cgtgagccag tcgcggacgt actgcttgtc gaagctgggt
     1381 tgcggcccgc cgggtgcgta gcgggcggcg tcccagaagc gcgagctatc gggcgtcagg
     1441 agttcgtcga tcaggatcag ttggtcgtcc agacggccaa actccatctt ggtgtcggcg
     1501 atgatgatgc cgcgggcgcg ggcataggcc gcggcctcgc ggtagatttg caggctggcg
     1561 gcgcgcagcg cctcggccat ctccgtgccc acgagcatga tcaggcgggc gaagctgatc
     1621 ggctcgtcat ggccggtcgc cgccttggtg gtcggggtga aaatcggctc gggcagttcc
     1681 tggctttcga ccaacccggc gggcagcgcc acgcccaggg cttcccgtcc gccgctctgc
     1741 cggtagtcgg cccaggccga gccggacagg tagccgcgca ccacgcactc gatgtcgata
     1801 cggcgggtgc gccggaccag catgctgcgg cccgccagct tggcccgctc gtcgggcggc
     1861 cagccgccgg tgtcgaaggg gtaggccgcc gggtcggtgg tgatgaagtg attgggcagc
     1921 agatcgcgcg tgcggtcgaa ccagaaggcc gacaggcggt tcagcacctc gccccgcccc
     1981 ggaataccgc tcggcagcac cacgtcgaag gcggagaggc ggtcggtcgc caccagcagc
     2041 aggcggttgc ccagttcgta ggtgtcgcgc accttgccgc ggagaaacag cggtaagggg
     2101 aaatcggtgg tcgtcaccgc cgcgtcaggg ccgggtgtgt tgctcaacag aactcctcgc
     2161 gcccttgccg gaccggcggc ggcgcgtata ataagagggt gcggtcccga ttattagcga
     2221 aagggtacgt tgtggcttct actcctggct cggaagagcc gccatcctcg ccccgcgagg
     2281 agcccccggc gcccgccgat ttgatgcaca acgagcgcga gcagttgcgc atcttcggcg
     2341 tgccggcgat ttacatcgcg ctgatcgcct tcatcatcct gatcgccatc atggcctggc
     2401 taggctggct ccccacttcc gtgctgtagc gattccggct cggcgggggg tgcgcccgcg
     2461 ttgcctgcgg cgcggacgcc gtcggcggtg cgtaactggg cggcctcggc gtcgagcgcg
     2521 gctttgcgcg ccagagcgcg ctgccgggcg ctcttgcggg cgcgtccccg caggctgatc
     2581 gtgtaggcga acagcggcat caggatcagc caggcgatga tgagcgccgg gcgggcgccc
     2641 gtgaaatcgg cgacagtgcg cgcgaagacg gcctgactca gccccacgca ggcgaacacg
     2701 aggccgatga tcccggcgat cagcaagatt ttgcgcgtgc gcacgtcagg cacggtccgt
     2761 gtcctctccc tcgggcagcg gcgcctcatt agcggctggt gccgctgcct gctcggcccg
     2821 cgcccggtcg aacaactgct gcagcgccgc gttgggccgc tccaattcgc cctgccagcg
     2881 cacgcggcgc aagcgcagat agatgcggcc aaaataggcc agcgcgccca ggctgaccag
     2941 cgccagcacc aggaagattg ccgcgcccag ggtgctgccg cgcagcccca ggcccagcga
     3001 ggccagcgtg gcgaacgtac ccacgccgat ggcgccctgg aaggcgaa
//
LOCUS       DASUYC010001098         3847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_420438,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001098 DASUYC010000000
VERSION     DASUYC010001098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420438"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="VF276_17905"
     CDS             complement(<1..746)
                     /locus_tag="VF276_17905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX6608779.1"
                     /translation="MTATDSHPPPTEPGPDSARATEAPGQTAWEHTVLEPALRRNPER
                     QASFETASGIPQERLYTPAGLQARNFDYEHDLGYPGAFPYTRGVQPTMYRGRFWTMRQ
                     YAGFGTAAESNARYRYLLSQGQTGLSVAFDLPTQMGYDPDHPLAEGEVGKVGVSIASV
                     EDMALLMEGIPLDQVSTSMTINATASILLAFYIAVARRQGVPEAKLDGTVQNDILKEY
                     IARGTYIYPPQPSMRIITDLFRYCADEVPR"
     gene            complement(743..1267)
                     /locus_tag="VF276_17910"
     CDS             complement(743..1267)
                     /locus_tag="VF276_17910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX6608780.1"
                     /translation="MERSYIVEVDGAEWQIAIRESPEGLRIRINDEPEQAVDYILAQI
                     PALYSLLLNGESSQARVVPSADEPAMWHVTLSGYHLDARVQTERERRLSRVTLGKQAH
                     TGEVTIKAPMPGLVRAVTVGVGDTVEQGQRLVLLEAMKMENEIHAPRAGTVKAVRVTP
                     GETVENGRPLVVIE"
     gene            complement(1278..2807)
                     /gene="accC"
                     /locus_tag="VF276_17915"
     CDS             complement(1278..2807)
                     /gene="accC"
                     /locus_tag="VF276_17915"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX6608781.1"
                     /translation="MPIRKLLIANRGEIAVRIIRACRDLGIASVAVYSDADRTALHVR
                     QADEAYPIGPAPARDSYLRADVMIDVARRCGADAIHPGYGFLSERAEFAEACRDAGLV
                     FVGPPPEAIRLLGDKVSARQLAIANDVPVVPGTEAEIRDPVEAARIAEQIGYPVLLKA
                     SAGGGGKGMRVVRTPADLEDAIRAASSEASAAFGYGGVYIEKYLEHVRHIEIQLLADS
                     HGNCIHLGERECSIQRRHQKLIEESPSPVLDPETRAAMGAVAVRAAKAAGYVNAGTIE
                     FLFTPERHFYFLEMNTRLQVEHPVTELVTGLDLVQEQLRVAAGEPLGYTQDDIRFHGH
                     AIECRVTAEDPEHNFRPSIGRIPAVSEPSGPGVRVDSGVYPGAEVSIYYDPMIAKVIC
                     WGSDRAQAMRRMRRALAEYRAAGILTNIPYQQAVIDSPEFERAEFDTGFVERFGPLAS
                     HTDPDDRARAARLAAVAAALTAHQAREKSAVHPVHGEAAPTAEDGRQWRAVGRRLSFH
                     V"
     gene            complement(2794..3198)
                     /locus_tag="VF276_17920"
     CDS             complement(2794..3198)
                     /locus_tag="VF276_17920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008880495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6608782.1"
                     /translation="MIRIETIDHVQILAPTGSEADIRAFYGGILGLEETEKPPELRQR
                     GGVWYRVGDGRILLHIGTEANPPDAGRRHFAFRVADVAAARRHLEDHGVRTGEAPQVA
                     GMPRFYGYDPFGNQIEFMNYADPQADPTHAHS"
     gene            complement(3195..>3847)
                     /locus_tag="VF276_17925"
     CDS             complement(3195..>3847)
                     /locus_tag="VF276_17925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX6608783.1"
                     /translation="VQEHFARNIVVGFARLNGRSVGIVAQQPMVLAGVLDIDSADKAA
                     RFVRFCDCFNIPIITFVDVPGFLPGVSQEHGGIIRHGAKLLYAYCEATVPKITVITRK
                     AYGGAYDVMSSKHVRGDINYAWPTAEIAVMGVDGAVNIIFKDQIEQSANPAATRAQLV
                     ADYQAKFANPYVAAGRGYVDDVIEPRTTRPRLIDALEMLKTKRDSNPPKKHGNMPL"
BASE COUNT          543 a         1211 c         1430 g          663 t
ORIGIN      
        1 cagcgcggaa cctcgtcggc gcagtagcgg aaaaggtcgg tgatgatgcg catcgagggc
       61 tgcggcggat aaatgtaggt gccgcgggcg atgtactcct tgaggatgtc gttctggacc
      121 gtgccgtcga gtttggcctc gggcacgccc tggcgccggg ccaccgcgat gtagaaggcc
      181 agcaggatcg acgccgtggc gttgatcgtc atcgacgtgc tgacctggtc gagcgggatg
      241 ccctccatga gcagggccat gtcttcgaca gaggcaatcg agacgccgac cttgccgacc
      301 tcgccctcgg ccagcgggtg gtcgggatcg tagcccatct gggtcggcag gtcgaaggcg
      361 acggaaaggc cggtctgccc ctggctcagc aggtagcgat agcgggcgtt ggactccgcg
      421 gcggtgccga acccggcgta ctggcgcatg gtccagaagc gcccgcggta catggtgggc
      481 tgcacgccgc gggtgtaggg gaacgcgccg gggtagccca ggtcatgctc gtaatcgaag
      541 ttgcgggcct gaaggccggc gggcgtgtag agccgctcct gcgggatgcc ggaagcggtt
      601 tcaaagctgg cctggcgttc ggggttgcgg cgcagcgcgg gttccagcac ggtgtgttcc
      661 caggcggtct ggccgggggc ctcggtggcg cgggcggagt ccgggccagg ttcggtcggt
      721 ggagggtgcg aatcggtggc agtcattcga tcaccaccag tgggcggccg ttttcgaccg
      781 tttcgcccgg cgtcacgcgt acagccttga ccgtgccggc gcgcggggca tggatttcgt
      841 tttccatctt catcgcctcc aggagcacga gccgttgccc ttgctccacg gtgtcgccta
      901 ccccgacggt gacagcccgg acgaggccgg gcatgggcgc tttaatggtg acttcgccgg
      961 tatgcgcctg tttgccgagc gtgacccggc tgaggcgccg ttcgcgttcg gtctgcacgc
     1021 gcgcgtcgag atggtagccg ctcagcgtca cgtgccacat ggccggttcg tccgccgagg
     1081 gcaccacgcg ggcctggctg ctctcgccgt tcagcaacag cgaatagagc gcgggaatct
     1141 gggcgaggat ataatcgacc gcctgctccg gctcatcgtt gatgcggatg cgcaagccct
     1201 ccggactttc gcggatggcg atctgccatt cggcgccgtc gacttcgaca atgtaggaac
     1261 gttccatcag actcctgcta gacgtggaag gataggcggc ggcctaccgc gcgccattgc
     1321 cggccgtctt cggctgtggg ggccgcctcg ccgtgcaccg ggtggacggc gctcttctcg
     1381 cgcgcctggt gtgcggtcag ggccgcggcc accgctgcca gccgggcggc gcgcgcccgg
     1441 tcgtcgggat cggtatggct cgccaggggg ccgaagcgct cgacaaagcc ggtgtcgaac
     1501 tcggcccgct cgaactcggg cgagtcgatg accgcctgct gatagggaat attggtgagg
     1561 atgcccgccg cgcggtactc ggcgagggcg cggcgcatgc ggcgcatggc ctgcgcccgg
     1621 tcgctgcccc agcagatgac cttggcgatc atcgggtcgt agtagatgga gacctcagcg
     1681 ccgggataga cgccgctgtc cacgcgcacg cccggtccgg acggttcgct gaccgccggg
     1741 atgcgcccga tcgatgggcg gaagttgtgc tccgggtcct ccgccgtcac gcggcactcg
     1801 atggcgtggc cgtggaagcg gatgtcgtct tgcgtgtagc ccagcggttc gcccgcggcg
     1861 acgcgcaact gctcctgcac cagatccagc cccgtgacca gctccgtgac cgggtgctcg
     1921 acttgcaggc gggtgttcat ctccaggaag tagaaatggc gctccggcgt gaacaggaac
     1981 tcgatggtcc cggcgttgac atagcccgcg gctttggcgg cgcgcaccgc gacggcgccc
     2041 atggcggcgc gcgtctccgg gtcgagcacg ggcgaggggc tttcctcgat cagtttctgg
     2101 tggcggcgct ggatcgagca ttcgcgctcg cccaggtgga tgcagttgcc gtggctgtcg
     2161 gccagcagtt ggatctcgat gtggcggaca tgctccaggt acttctcgat gtagacgccg
     2221 ccgtagccga acgcggcgct ggcctcgctg gaggcggcgc ggatggcgtc ctccaagtcc
     2281 gcgggggtgc gcaccacgcg catgcctttg ccgccgccgc ccgccgacgc cttgagcagc
     2341 accgggtagc cgatctgctc ggcgatgcgc gcggcttcca cggggtcgcg gatctcggcc
     2401 tcggtgcccg gcaccacggg cacgtcgttg gcgatggcaa gctggcgggc ggagaccttg
     2461 tcgcccagca ggcggatcgc ttcgggcggc ggcccgacga atacgaggcc cgcgtcgcgg
     2521 cacgcttcgg cgaactcggc gcgctcggac aggaagccgt agccgggatg aatcgcgtcg
     2581 gccccgcagc gccgggccac gtcgatcatc acgtcggcgc gcaggtagct gtcgcgggcg
     2641 ggcgccgggc cgatggggta ggcttcgtcg gcctggcgca catgtagcgc ggtgcggtcg
     2701 gcgtcggaat agacggccac gctggcgata cccaggtcgc ggcaggcgcg aatgatgcgc
     2761 accgcgatct cgccgcggtt ggcgatcagt agcttacgaa tgggcatggg tcgggtccgc
     2821 ctgcgggtcg gcgtagttca tgaactcgat ctggttgccg aaggggtcgt agccgtagaa
     2881 gcgcggcatc ccggccacct gcggagcctc gccggtgcgg acgccgtggt cctccaggtg
     2941 gcggcgggcc gcggccacat cggccacgcg gaaggcgaag tggcggcgcc cggcgtcggg
     3001 cgggttggcc tcggtgccga tgtgcagcaa gatgcgcccg tcgcccacgc ggtaccagac
     3061 gcccccgcgc tggcgcaact cgggcggctt ctccgtctct tcgaggccca ggatgccgcc
     3121 gtagaaggcg cggatgtccg cctcgctgcc ggtgggcgcc aggatctgga cgtggtcgat
     3181 ggtttcgatg cggatcataa gggcatgttc ccgtgcttct tgggcgggtt ggagtcccgc
     3241 ttggttttaa gcatttcgag ggcgtcgatc agccggggcc gcgtggtgcg cggctcgatc
     3301 acgtcgtcca catagccacg cccggcggcc acgtaggggt tggcgaactt ggcctggtag
     3361 tcggcgacca actgggcgcg cgtggcggcg gggttggccg actgctcgat ctggtccttg
     3421 aagatgatgt tgaccgcgcc gtcgacgccc atcacggcga tctccgccgt gggccaggcg
     3481 tagttgatgt caccgcgcac gtgcttgctt gacataacgt cgtaggcacc gccgtaggct
     3541 ttgcgcgtga tcaccgtgat cttgggcacc gtggcctcgc agtaggcgta gagcagcttg
     3601 gcgccgtggc ggatgatgcc gccgtgttcc tgcgacacgc cgggcaggaa gccgggcacg
     3661 tcgacgaagg tgatgatcgg gatgttgaag cagtcgcaga agcgcacgaa gcgcgcggct
     3721 ttatcggcgg agtcgatgtc gagtacgccg gccagcacca tcggctgctg ggccacgatg
     3781 ccgaccgagc gcccgttgag ccgggcgaag ccgacgacga tgttgcgcgc gaaatgctcc
     3841 tgcactt
//
LOCUS       DASUYC010001099         2548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1115056, whole genome shotgun sequence.
ACCESSION   DASUYC010001099 DASUYC010000000
VERSION     DASUYC010001099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115056"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..767)
                     /locus_tag="VF276_17930"
     CDS             complement(30..767)
                     /locus_tag="VF276_17930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608784.1"
                     /translation="MKAFVSAMARWFMGLAGGKGLHYGMGTRLRMPGAAMLMVAALAL
                     LPSLAAAQSLATPPRRIEFNLKANARFVNCLAQYPGDTRRPPKAEVEVKRGRLNDELE
                     LKLQNIKPGLAFDLFTVQRSSLLANGTPDPNFTNFGMAWYQSDVQANSEGSAHVEINT
                     ILLDQIFGFDPDANLAPTNTFHVGFWFNNPQDAAACGFDVTHPTPFNGEHRAGPLAMI
                     SLPDAATNLGPLCSNPDRSVTPARCNP"
     gene            complement(1230..1934)
                     /locus_tag="VF276_17935"
     CDS             complement(1230..1934)
                     /locus_tag="VF276_17935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608785.1"
                     /translation="MPNPNTRRARGLDVLVLVVAGLVNTVLAPADAALVLAPGTPAQG
                     GNLTVRMAWVTAHADQWQAGWAFWFVVTTTFAWSYYALARHLGGPLPWRTLAVWLALA
                     AAAVDLAGVLLNLGLLPQLAQRAQEVPVEAQQPAALTYLAAETLANQLTTAAYGLYTG
                     AGLLLLPALVATPAYPRPLVWLGAAEWAVATVTTALLVLAPALATGPLLLSFLLYAPW
                     VWGSAWWIAHPGRPAR"
     gene            complement(1921..>2548)
                     /locus_tag="VF276_17940"
     CDS             complement(1921..>2548)
                     /locus_tag="VF276_17940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608786.1"
                     /translation="TWARARVTLVVITLFSVAATLVTLLNLGPFLKHPWFHLAYWLTM
                     YCILFIAAPVVFITQERAHGGRLPVEVPLSPPARALAAFGLAACGLTGLGLLANVALV
                     NPFWPWTLTPLVGGIVGVWFTTLAAGYAWTLWDGDWRRTRIMFQQAIPTGLGLALLPL
                     LHAGDVRPGAGGLVALYLALALLPAAGALILLLTMRRAPLAEVGRAEP"
BASE COUNT          425 a          825 c          952 g          346 t
ORIGIN      
        1 ccacgccggt agccgcgggt cgcgctagtc tacgggttgc agcgggccgg ggtcaccgag
       61 cggtcggggt tgctgcagag cggccccagg ttggtcgccg catcgggcag gctgatcatt
      121 gccagcgggc cggcacggtg ctcgccgttg aagggcgtcg ggtgggtcac gtcgaagccg
      181 caggccgcgg cgtcttgcgg gttgttgaac cagaagccga cgtggaaggt gttggtcggc
      241 gccaggttgg cgtcgggatc gaagccgaag atctgatcca gcaagatggt attgatctcg
      301 acatgggcgc tgccctcgct gttcgcctgg acgtcggact ggtaccaggc catgccgaag
      361 ttggtgaagt tcgggtcggg tgtgccgttg gcgagcagcg agctgcgctg cacggtgaag
      421 agatcaaagg ccaggccggg cttgatgttc tggagtttca gttcgagctc gtcgttcagc
      481 cggccccgct tgacttcgac ctcggccttg ggcgggcggc gggtgtcgcc gggatactgg
      541 gccaggcagt tgacgaagcg cgcgttggcc ttgaggttga actcgatgcg ccggggcggc
      601 gtggcgaggc tctgggcggc ggccagcgac ggcagcagcg ccagcgcggc gaccatcagc
      661 atggccgcgc ccggcatgcg cagccgggta cccatgccgt agtgcagccc cttgccgccg
      721 gccaacccca taaaccaccg tgccattgcc gatacgaatg ctttcatctt gtatctcctt
      781 aactttagtt gttgtcggtt gcttgctatt ccactcccgc ttgcgggcag ccggcaagcg
      841 gaatcacaat cgatgccgaa tggccgcatg ttgtcgaaac gctcggtgtg cgatgcctca
      901 cagttacctc ctcgatccag atttcggctc ggccaggagc gtttgcacac caccacgata
      961 cccgcaaccg ccgctcggca catgcgggat tccccggtat tgcgcgggcc gccagtacgg
     1021 taatcgcggg gtggggcagc accgtaacca tatcagggca cggggcagcg gaccgaccag
     1081 cagagcgaca tccgggtacc cgaccaacct aaccccccta ccccccttcc cgtcgcggga
     1141 agggaggaat cgtttgcgcg agaaaaagta actcccctcc ccgcggcggg gaggggccgg
     1201 gggagaggtt ggttggtttg agcggtgggt tagcgcgcag gccggcccgg atgcgcaatc
     1261 caccaggcgc tgccccagac ccagggcgcg tagagcagga agctcagcag cagcggcccg
     1321 gtcgccaggg cgggggccag gaccagcagt gcggtcgtca cggtggcgac ggcccactcg
     1381 gcggcgccca gccagacgag cgggcggggg tacgcgggcg tggcgacgag ggcgggcagc
     1441 aggagcaggc ccgcgccggt atagagccca tacgcggcgg tggtgagttg gttcgccagc
     1501 gtctcggcgg cgagatacgt gagggcagcc ggttgctggg cctcgacggg gacctcctgc
     1561 gcccgctggg cgagctgcgg gagcaggccg aggttgagca gcacgccggc cagatcaacc
     1621 gccgccgctg ccagcgccag ccagacggcc agggtgcgcc acgggagcgg cccgccgagg
     1681 tgccgcgcga gcgcatagta gctccaggcg aaggtagtgg tcacgacgaa ccagaaggcc
     1741 cagccggcct gccactggtc cgcgtgcgcc gtgacccagg ccatgcggac cgtcaggttg
     1801 ccgccctgcg ccggcgtgcc gggggccagc accagcgcgg cgtcggcggg ggcgagcacg
     1861 gtattgacca ggcccgcgac gaccaggacc agcacgtcga gcccgcgggc ccggcgggtg
     1921 ttagggttcg gcacgcccga cctcggccag cggcgcgcgg cgcatggtca gcagcagaat
     1981 cagggcaccc gccgcgggca gcagggccag ggccaggtag agcgcgacca ggccccccgc
     2041 ccccggccgc acatcgccgg catggagcag cggcagcaac gccaggccca gcccagtggg
     2101 gatggcctgc tggaacatga tgcgcgtgcg ccgccaatcg ccgtcccaca gcgtccaggc
     2161 atagcctgcg gccagcgtgg tgaaccacac cccgacgatg ccgccgacca gcggcgtcag
     2221 cgtccacggc cagaacgggt tgacgagagc gacgttggcg agcaggccca ggcccgtcag
     2281 cccgcaggcg gcgaggccga aggcggccag ggcgcgggcc ggcggcgaca gcggcacctc
     2341 gacgggcagc cgcccgccgt gggcgcgttc ctgggtgata aacacgaccg gcgcggcgat
     2401 gaacaggatg cagtacatgg tcagccagta ggccaggtgg aaccaggggt gcttgaggaa
     2461 cggccccagg ttgagcaagg tgacgagcgt ggcggcgacc gaaaacaggg tgatcacgac
     2521 aagggtcacg cgcgcccgcg cccaggtg
//
LOCUS       DASUYC010001100         2128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1806996, whole genome shotgun sequence.
ACCESSION   DASUYC010001100 DASUYC010000000
VERSION     DASUYC010001100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1806996"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="VF276_17945"
     CDS             complement(<1..845)
                     /locus_tag="VF276_17945"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX6608787.1"
                     /translation="MAASKVTVLAYHRIAISGHPADLSPALITSNPAAFEAQMRFVAA
                     RYNVISSWDLVRALRDGKALPPRALIITFDDGYRCFKDTALPILRRLNLPATLFVPTG
                     FPGVPGRLFWWDAIHRALARTREPQIEVPGLGVLPLTTPVERRAAYDRLVPLVERTPE
                     AAAGRLVESVVAQCKVEPNTVPHMLDWDELAAVAEQGVDIGPHTREHIVLAQASPERV
                     AAEVEGSWADLRARIPRALPIFCYPTGKPHAINCVAANAVRQAGLAGAYTMVAGLNVI
                     GQTDP"
     gene            complement(739..>2128)
                     /locus_tag="VF276_17950"
     CDS             complement(739..>2128)
                     /locus_tag="VF276_17950"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX6608788.1"
                     /translation="PIYAVLIYADLLSVLTHYQGLPPLARFVGAALLAAVLGYRLVIH
                     RQGLVADEVTWWMLLYGLMVSVGLLYARDSDPVMTNIIEFARNFITYLIIVNTATTRT
                     RLRRILWALLAAGTILSLLTIYQSLTGNWANEFGGLAQSRATEIVNGQAAPRPGGTIN
                     DANYYGLSLIVLVPLALYLVFEGKRLLVRLVGGASALALIAAIVFTYSRGDALALAIL
                     LGAVALYKRLNPLYLLGIAVALLIAVPILPPNYVARLSTVLQTAEGNQQTLYNETSIR
                     GRAGAVQSAIAMFLDHPLVGVGRENYVLYELEYISGTSLALDAKGIPPHDLYLEVLAE
                     HGLMGLFVVGGLFVMLWRAVLEAHRRFRAVGNHPDAELAAWLGLGLFGYMAGSLFLHG
                     SYFYLFWLQMAAIVALRQLSRTAPADGRDDGKEHTHGGFEGDGAGVSPDRHFRPPRRL
                     IPRTHYIEPGSF"
BASE COUNT          383 a          719 c          721 g          305 t
ORIGIN      
        1 taggggtcgg tctgcccgat cacgttgaga ccggcgacca tagtgtaggc gccggccagc
       61 ccggcctggc gcacggcgtt cgcagccacg cagttaatcg cgtgcggttt cccggtggga
      121 tagcagaaga tcggcagcgc ccgcgggatc cgcgcccgca ggtcggccca cgacccctcc
      181 acctcggcgg ccacgcgctc gggactcgcc tgcgccagca cgatgtgttc gcgcgtatgc
      241 gggccgatgt ccactccctg ctcggccacc gcggccaact cgtcccagtc cagcatatgc
      301 ggaacggtgt tcggctcgac cttgcattgc gcgaccacgc tctccaccag gcggcccgcc
      361 gcggcttcgg gcgtccgctc gaccagcggc acgagccgat catacgcggc gcggcgctcc
      421 accggggtgg tcaggggcag cacgcccagg ccgggcacct cgatctgcgg ctcccgcgtg
      481 cgcgccagcg cacggtgaat ggcgtcccac cagaagagcc ggccgggcac gccggggaac
      541 cccgtgggca cgaagagcgt ggccggtagg ttaagccggc gcaagatcgg cagcgcggtg
      601 tccttaaagc agcgatagcc gtcgtcgaag gtgatgatca gggcgcgggg cggcagggct
      661 ttgccgtccc gcaacgcgcg caccaggtcc cacgacgaga tcacgttgta gcgcgcggcc
      721 acaaagcgca tctgggcctc aaaagctgcc gggttcgatg taatgagtgc gggggataag
      781 tcggcggggt ggccggaaat ggcgatccgg tgatacgcca gcaccgtcac cttcgaagcc
      841 gccatgcgtg tgctcctttc cgtcgtcacg cccatccgcg ggcgccgtgc gggacaactg
      901 gcgcagcgcc acaatcgcgg ccatctgcaa ccagaacaga tagaagtatg acccgtgcag
      961 gaacaggctg ccggccatgt agccgaacaa cccgagcccc agccaggccg ccagttccgc
     1021 gtcgggatgg ttgcccaccg cgcggaagcg ccggtgggct tcgagcaccg cgcgccagag
     1081 catcacaaag aggccgccga cgacgaacag gcccatcagg ccatgctcgg ccagcacctc
     1141 caggtagagg tcgtgcggcg ggatgccctt cgcgtccagc gccaggctcg tgccggagat
     1201 atattccagt tcgtacagca catagttctc gcggcccaca ccgaccagcg ggtggtccag
     1261 gaacatggcg atggccgatt gcacggcgcc cgcccgcccg cgaatggacg tttcgttgta
     1321 cagcgtttgc tggttgccct cggcggtttg cagcacggta ctgagccggg ccacgtagtt
     1381 cggcggcaaa atgggcaccg cgatgagcag cgccacggcg atgcccagta gatagagcgg
     1441 gttcaggcgc ttgtagagcg ccaccgcgcc cagcaagatt gccagcgcca gggcgtcgcc
     1501 gcgcgagtag gtgaagacaa tggcggcgat gagcgccagg gcgctggccc cgcccaccag
     1561 gcgcaccagc aagcgcttgc cctcgaacac caggtagagc gccagcggca ccaggacgat
     1621 caggctcaac ccgtagtagt tcgcatcgtt gattgtgcca ccggggcgcg gggccgcctg
     1681 cccgttcaca atctccgtcg cccgcgattg cgccaggccg ccgaattcgt tggcccagtt
     1741 gcccgtcagc gactggtaga tcgtgagcag cgacagaatg gtgcccgcgg cgagcagcgc
     1801 ccagaggata cggcgtaggc gcgtgcgcgt cgtggccgtg ttgacgataa tcaggtaggt
     1861 gatgaagtta cgggcaaact cgatgatatt ggtcatcacc gggtcggagt cgcgcgcgta
     1921 caacaggccg acgctgacca tcagcccata gagcagcatc caccacgtca cttcgtcggc
     1981 caccagcccc tgccggtgga tcaccagccg gtagccgagc actgccgcca gcagggcggc
     2041 gcccacgaag cgggccagcg gcggcagccc ctggtagtgg gtcagcacgc tgagcaggtc
     2101 ggcgtagatc agcaccgcat agatcggc
//
LOCUS       DASUYC010001101         2720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1343483, whole genome shotgun sequence.
ACCESSION   DASUYC010001101 DASUYC010000000
VERSION     DASUYC010001101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343483"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(228..2105)
                     /locus_tag="VF276_17955"
     CDS             complement(228..2105)
                     /locus_tag="VF276_17955"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEX6608789.1"
                     /translation="MNRAPDLAPAPSAPRGTRQTAEASSPAPAPAARRAPLLHGLVLA
                     LIVVVALVLRIVGHNWDENQHLNVDDYYVTKVAMTRVNLPPDASLGQLLDPATSPLNP
                     RAGGEFYVYGALPLYAARAAGALATAATGNTYYSSLNGVEQIGRALAALFDTLTLLLV
                     YLTARRLWGAGAGLFAAVLYALAVLPIQISHFYITDPFMTTFMTATLAASVLFYQTRR
                     LRFAALAGLCTGLALACKLSAAPVVVVPGAAALALWWQARAAGEGRRAARRLVATGAL
                     IAGAAFLGLLLADPFAVLDAGSYLRIMTEQATIQTGDKDQWFTRKYVGTWPVLYLGSQ
                     LLLLGAGPLVGLAGLAGGGLVAARAWRGRRVVAGLLLLGALAYFASIAFAETQWVRYL
                     LPVVPYLCLFATAFALWSADWRAGGAWTVRRAVVPGVLLLSAALGALAISATFQRPHP
                     QVQASRWIYDHVPYRTPLGIEVTANVMPLPLPGHVAPAREYPLMRFDPLADQSSPAAA
                     AALRAWLNSADYLIIDASQAARIAPHLPWRYPVQIRYYDLLFQGRLGFTPVYIATSYP
                     SLFGLQLRDDVAWIDPSFIEFSHPPIWIFHKDRTLSDAEWQALFADAVVQPAVPSRHA
                     P"
     gene            complement(2102..>2720)
                     /gene="yqeC"
                     /locus_tag="VF276_17960"
     CDS             complement(2102..>2720)
                     /gene="yqeC"
                     /locus_tag="VF276_17960"
                     /inference="COORDINATES: protein motif:HMM:TIGR03172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HEX6608790.1"
                     /translation="VPAHPFVIEPDPAALAAQLPGLLAEAGHVRVAGEQLRVDKIRGL
                     DAAQVAALKTLPGLDNLLVEADGARHRSLKAPAAHEPALPAGVDLLLIIVGLDIIGKP
                     LDEDHVHRPEQVAAITGLPMGAILTPAAVAKVVTSPAGGLKDVPPGVRGWVVATKFSY
                     DTAPVGGELASAVLTAGDSRIEGVVLLGAGPVEQIGSAPMVVVKW"
BASE COUNT          445 a          955 c          988 g          332 t
ORIGIN      
        1 cccggttgcg cccaccgcgg tccccgaccg gggcatcacg ccagacatcg tgttgccgcg
       61 caccaacgac gacgtagccc agcagcgcga cccgcaactc gaccgcgccg tgcagttcgt
      121 cctcaccggc aagtaggtca ccgggctacc gcgcatacga tcaggggcgg cgccggatgt
      181 gggcgccgcc cttcgtattt ccatgcaaac gtactggcgg cgtgaaccta cggcgcgtgg
      241 cgcgagggga ccgcgggctg gacgacggca tcggcgaaga gggcctgcca ttcggcgtcg
      301 ctgagcgtgc ggtccttgtg gaagatccag atcggcgggt gcgagaactc gatgaacgag
      361 gggtcgatcc aggccacatc gtcgcgcagt tggagaccga acaggctggg gtacgacgtg
      421 gcgatgtaga ccggggtgaa gccgagccgt ccctggaaca gcaggtcgta gtagcggatc
      481 tggaccgggt agcgccaggg caggtgcggg gcgatgcgcg ccgcctggct ggcgtcgatg
      541 atcaggtagt cggcgctgtt gagccaggca cgcagggccg cggcggctgc tgggctggac
      601 tggtcggcca ggggatcgaa gcgcatcaac gggtactcgc gggcgggggc cacgtggccc
      661 ggcagcggca gcggcatcac gttcgccgtg acctcgatgc ccagcggcgt gcgatagggc
      721 acatggtcgt agatccagcg cgacgcctgc acctgcgggt gcgggcgctg gaacgtcgcg
      781 ctgatcgcca gcgcgccgag cgccgccgac agcaggagga cgccgggcac gaccgcccgg
      841 cgcaccgtcc aggcaccgcc cgcccgccaa tccgccgacc acagcgcaaa ggctgtcgca
      901 aagaggcaga gatagggcac gaccggcaac aggtagcgca cccactgtgt ctcggcaaag
      961 gcgatggacg cgaaataggc cagggcgccc agcagcaaca gccccgcgac cacccgccgt
     1021 ccgcgccagg cgcgcgccgc caccagcccg ccgcctgcca gcccggccaa ccccaccagc
     1081 ggcccggcgc ccagcagcag caactggctg cccaggtaga gcaccggcca ggtgcccacg
     1141 tatttgcgcg tgaaccactg gtccttgtcg ccggtctgga tggtggcctg ctcagtcatg
     1201 atgcgcaaat agctgcccgc atcgagcacg gcgaacgggt cggccagcag caggcccagg
     1261 aacgcggcgc ccgcgatcag cgcgcccgtc gcgaccagcc ggcgggccgc gcgccgcccc
     1321 tcgcccgccg cccgcgcttg ccaccagagc gcgagcgccg cggcgccggg cacgacgacc
     1381 accggggccg cgctgagctt gcaggccagg gccagcccgg tacacagccc ggccagcgcg
     1441 gcaaagcgca gccgccgggt ctggtagaac agcacgctgg ctgccagggt cgccgtcatg
     1501 aaggtggtca tgaacggatc ggtgatgtaa aagtggctga tctggatggg caggacggcc
     1561 agggcgtaga gcaccgccgc gaacaggccc gcccccgcgc cccagagccg ccgcgccgtc
     1621 agatagacca gcagcagcgt gagcgtatcg aacagcgccg ccagggcccg cccgatctgc
     1681 tcgacgccgt tgagcgagct atagtaagtg ttgccggtcg cggcggtcgc cagggcaccg
     1741 gcggcacgcg cggcgtagag cggcaaggcg ccgtagacgt agaactcgcc gcccgcgcgc
     1801 gggttgagcg ggctggtagc cgggtcgagc aactgcccca gggaggcatc cggcggcagg
     1861 ttgacgcggg tcatcgccac cttggtcacg tagtagtcgt cgacgttgag gtgctggttc
     1921 tcgtcccagt tgtggcccac aatgcgcagc accagggcca cgaccacgat cagcgccagg
     1981 accagcccat ggagaagcgg cgcgcgtcgg gcggcgggtg cgggcgccgg ggatgaagcc
     2041 tctgccgtct gcctcgtgcc ccgcggagcg gagggcgcgg gggcgaggtc cggcgcgcgg
     2101 ttcaccactt gaccaccacc atgggcgccg aaccgatttg ctcgaccggc ccggcgccga
     2161 gcaacaccac gccttcgatg cgcgagtcgc ccgccgtcag caccgccgag gccagttcac
     2221 cgcccacggg ggccgtgtca tagctgaact tggtggcgac cacccagccg cggacgcccg
     2281 gcggcacatc cttcagcccg cccgccgggc tggtcacgac cttcgccacg gcagcggggg
     2341 tgaggatcgc ccccatcggc aggccggtga tcgcggccac ctgctcgggg cgatggacgt
     2401 ggtcttcgtc cagcggcttg ccgataatat cgaggcccac gatgatcagc agcagatcga
     2461 cccccgcagg cagggccggt tcgtgggcgg cgggcgcctt gagcgagcgg tggcgcgcgc
     2521 cgtcggcctc gaccagcagg ttatccaggc cgggcagcgt tttgagcgcg gccacctgcg
     2581 cggcgtccag tccgcggatc ttatcgacgc gcaactgctc gcccgcgacg cgcacgtgcc
     2641 cggcttcggc cagcaggccc ggcagttgcg ccgccagcgc cgccgggtcg ggttcgatga
     2701 caaagggatg cgcgggcacg
//
LOCUS       DASUYC010001102         2024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_884518,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001102 DASUYC010000000
VERSION     DASUYC010001102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884518"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..332)
                     /locus_tag="VF276_17965"
     CDS             complement(84..332)
                     /locus_tag="VF276_17965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608791.1"
                     /translation="MPWPNITTVAQCRARLAESRRMRLASLDLWPVPPHLETEFHYHP
                     DGAPVNAYERFMGGLRHANSHIDQIHEIVRQARARAEC"
     gene            complement(338..>2024)
                     /locus_tag="VF276_17970"
     CDS             complement(338..>2024)
                     /locus_tag="VF276_17970"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEX6608792.1"
                     /translation="QPPLYYALCAAVAVAGRALTGADLGAPLTTPLQANPAWFEYTSS
                     LAMLHPAADRWPLWPFVFRGLSILAGLGVVGLTYATARTLLPPPAPAAGPLIAAAFAA
                     LLPQANFIRASVSNETLAALIGAWIVLLLVQHLMRPYSRRRLVWLGVALGLGLLTKAS
                     TVALWPAVGAVLLIHRPVPWRPWLGDLVRLAAVAGVLAGPFYLWRWAVYGDPTAAAAW
                     AEMLPPDSPWHLTDLFWLVDPFREALWQSFWGVFGWQNVPLPVGFYYAFLGVTLLAVG
                     GGAYLVARRALSRAQQAACGVLVAVMLLLYLLVILFSLRLVAWQGREMFPALSSICVL
                     FGVGLAGLALGRAAVRGGAALARGRRWLAARLAPAVALGLLGVNLFSIVYVVAPVLTP
                     PPIAPPATRALALAAVVGALRMPVAVPPGGEHTFADVPPTHPAFAAIETGAHAGLIAG
                     YTCGGPGEPCDAARRPYFRPDAPLVRGHLAKILVTARHWPLVTPAQSPFADMPPAAPL
                     YPYITTAAAHGVLSGMPCRAPRWPCDAAAQPYFGPSLVVFPAELEASLVLARR"
BASE COUNT          329 a          713 c          779 g          203 t
ORIGIN      
        1 accatcgcga tgagatcggc ggcgccattc acggtccccc accgccgcta taccacgcac
       61 aactcggtac tccgcatcgc tactcagcac tcagcacgcg cccgcgcctg gcgtacgatc
      121 tcgtggatct ggtcgatgtg ggagttggcg tggcgcaagc cgcccatgaa gcgctcatag
      181 gcgttgaccg gcgccccgtc ggggtgatag tggaactcgg tttccaggtg gggcggcacc
      241 ggccagaggt ccaggctcgc cagccgcatg cgacggcttt ccgccaggcg ggcgcggcac
      301 tgggcgacgg tggtgatgtt tggccacggc acgctgacta ccggcgcgcg aggacgaggc
      361 tggcttcgag ttcggcgggg aacacgacca ggctcgggcc gaagtagggc tgggcggcgg
      421 cgtcgcaggg ccagcgcggg gcgcgacagg gcatgccgct gaggacgccg tgagcggcgg
      481 cggtggtgat gtaggggtag agcggcgccg ccggaggcat atcggcgaag ggtgactggg
      541 ccggggtcac gagcggccag tggcgggccg tgaccaggat tttcgccagg tggccgcgga
      601 ccagcggcgc atcggggcgg aagtaggggc gccgggcggc gtcgcagggt tcgccgggac
      661 cgccacaggt gtagccggcg atcagcccgg cgtgggcgcc cgtctcgatg gcggcaaacg
      721 cggggtgcgt gggcggcaca tcggcgaagg tgtgttcgcc ccccggcggc acagcgaccg
      781 gcatgcgcag ggccccgacc acggcggcca gcgccagggc gcgggtggcc gggggtgcga
      841 tggggggcgg cgtgagcacc ggcgccacca cgtagacgat gctgaacagg ttcacgccca
      901 gcaggcccag cgcgaccgcg ggcgccagcc tcgcggccag ccagcgccgc ccgcgcgcca
      961 gggccgcccc gccgcgcacc gccgcccggc ccagcgccag cccggccagc cccacgccaa
     1021 acagcacgca gatgctcgac agcgccggga acatctcgcg gccctgccag gccaccagcc
     1081 gcaggctgaa caggatcacc agcagataga gcagcagcat gaccgcgacc agcaccccgc
     1141 aggccgcctg ctgcgcccgg ctgagcgctc gccgcgccac caggtaggcg ccgccgccca
     1201 cggccagcag cgtcaccccc agaaacgcat agtagaatcc gaccggcagc ggcacgttct
     1261 gccagccgaa cacgccccag aacgattgcc agagcgcctc acggaaggga tcgaccagcc
     1321 agaatagatc ggtcaggtgc caggggctgt cgggcggcag catctccgcc caggccgcgg
     1381 cggctgtggg gtcgccataa acggcccagc gccagaggta gaacgggccg gccagcacgc
     1441 ccgccacggc agccaggcgc acgagatcgc ccagccaggg gcgccacggc acggggcggt
     1501 ggatcagcag cacagcgccc accgccggcc agagcgccac ggtgctggcc ttggtgagca
     1561 ggcccagccc cagcgccacg cccagccaga ccaggcgccg ccggctgtag ggccgcatca
     1621 ggtgctgcac cagcagcagc acgatccacg cgccgatgag ggcggccagg gtctcgttgc
     1681 tcacactggc gcggatgaag ttggcctgcg gcagcagcgc ggcgaacgcg gcggcgatca
     1741 gcggccccgc cgcgggggcg ggcggcggca gcagcgtccg cgcggtggca taggtcagcc
     1801 ccaccacgcc cagcccggcc aggatggaga ggccgcggaa cacgaagggc cacagcggcc
     1861 agcggtccgc ggccgggtgc agcatcgcca ggctggaggt gtactcgaac caggccgggt
     1921 tggcctggag gggggtcgtc agcggggcgc ccaggtcggc cccggtcagg gcgcgcccgg
     1981 ccacggcgac ggcggcgcag agggcgtagt agagcggtgg ctgc
//
LOCUS       DASUYC010001103         3178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1201296, whole genome shotgun sequence.
ACCESSION   DASUYC010001103 DASUYC010000000
VERSION     DASUYC010001103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1201296"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VF276_17975"
     CDS             complement(<1..466)
                     /locus_tag="VF276_17975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HEX6608793.1"
                     /translation="MATQYRCRNQARRIAVRETLGGDGQPLRNGIDFLEVGADQQTLR
                     VHFIHNLPVSPDPVLTRDNVRIDGGVRITGIKVTAVNSVEDVLTVVTDRAGDFSTYTL
                     RLVTSPTNPAPPPPMDVQLSEVPFTFKVDCPSDFDCAPATACPPEAPPEPLID"
     gene            complement(470..826)
                     /locus_tag="VF276_17980"
     CDS             complement(470..826)
                     /locus_tag="VF276_17980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006514835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HEX6608794.1"
                     /translation="MQIDYPYHFDSRGRTATTDQDDHIRDLIEQVLFTVPGERVNRPT
                     FGTGLLQLVFAPNSDELATATQFLVQSALQQWLGDLIQVEAVAVENVDSTLRLTVQYT
                     VRRTQQRQVAQFSREV"
     gene            complement(826..1146)
                     /locus_tag="VF276_17985"
     CDS             complement(826..1146)
                     /locus_tag="VF276_17985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608795.1"
                     /translation="MPGFLLHVGATVLCAHGGQAQPTVPNPRVLVGGQPVVTQSGPYV
                     IAGCPFVPPGGNGPCVTAQWVTGATRVLAGGVPVLMQDSQAICAPTGTPLMIVVTQVR
                     VKGM"
     gene            complement(1146..1643)
                     /locus_tag="VF276_17990"
     CDS             complement(1146..1643)
                     /locus_tag="VF276_17990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEX6608796.1"
                     /translation="MSQFFGKYRGKVANNIDPMQLGRVQVSVPAVLGDGSLSWAMPCA
                     PYGGSGVGFFAVPPVGANIWVEFEGGDSDYPIWSGCFWGVGEVPAQPALPQMKVWKTD
                     GITLTLSDLPGAGGFTVEVGPPVVPTPLKMVFNASGIEISNSAASVKLTPASVSLNNG
                     ALEVI"
     gene            complement(1640..2776)
                     /locus_tag="VF276_17995"
     CDS             complement(1640..2776)
                     /locus_tag="VF276_17995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608797.1"
                     /translation="MLLGVHLTLLIGPTIAVPAPIQLTEALQSVRVSHSDDRQSGFEI
                     TFQIGRSGPADLLDYALVSNPLLKPFNRVVIVVTFSVMPRVLMDGIITHVQVSPSDQA
                     GASTMTVIGEDVSVMMDRKEESAEHPAQDETIIALKLIAKYAQYGLIPLVIPPPALDV
                     PIPIERTPVQQATDLQYLKQMAARYGYVFYITPGPAPLTNTAYWGPPIRAGVPQSALT
                     INMGPETNVQTFNVRNNALEPTVVEGKVQDRTTNQSLPVQTFASLRPPLAAFPAWVAN
                     AANLRHVQFRESGVNTAQAFARAQGTMDASVDAVVAVGDLDATRYGNLLQPRGLVGVR
                     GAGYLNDGFWYVKSVDHTVRRGTYQQHFTLSREGVGSTTPTVIP"
     gene            complement(2818..>3178)
                     /locus_tag="VF276_18000"
     CDS             complement(2818..>3178)
                     /locus_tag="VF276_18000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015121144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608798.1"
                     /translation="RPPGFGRCRGRFQPDPPPSGGQTMFDPTSRYYNIETAGLLMPDG
                     RTVAYVRRRFLPQGAAMPLLVEITVAQGDRLDLITARTLGDPEQFWRVADANDAMNPP
                     DLTAQVGRVLRVPIPQP"
BASE COUNT          483 a         1002 c         1159 g          534 t
ORIGIN      
        1 agtcgatgag cggctccggc ggcgcctccg gcgggcaggc cgtcgcgggc gcgcagtcga
       61 aatcgctggg gcaatcgacc ttgaaggtga acggcacctc gctcagttgc acgtccatcg
      121 gcgggggtgg cgcgggattg gtcggcgacg tgaccaggcg cagcgtgtag gtggagaaat
      181 cgcccgcccg gtcggtcacg accgtgagca cgtcttccac ggagttcacc gctgtgactt
      241 tgatgcccgt gatgcgcacg ccgccgtcga tgcgcacgtt gtcgcgcgtg agcaccgggt
      301 cggggctgac cggcaggttg tggatgaaat gcacgcgcaa cgtctgctga tcggcgccga
      361 cctccaggaa gtcgatgccg ttgcgcagcg gttggccgtc gccgcccagc gtttcgcgga
      421 cggcgatgcg gcgggcctgg ttgcggcagc ggtactgagt ggccatgaat cacacctcgc
      481 gggagaactg cgcgacctgg cgctgctggg tgcgccggac ggtgtactgg acggtcaggc
      541 gcagggtcga gtcgacgttt tcgacggcca cggcttcgac ctggatcagg tcgcccagcc
      601 attgttgcag cgccgactgc accaggaatt gggtcgccgt cgccagttcg tcgctgttgg
      661 gcgcgaagac gagttgcagc aggccggtgc cgaaggtggg ccggttgacg cgctcaccgg
      721 gcacggtgaa cagtacctgc tcgatcaggt cgcggatgtg gtcgtcctgg tcggtggtcg
      781 cggtgcgccc gcggctgtcg aaatggtatg ggtagtcgat ctgcatcaca tcccctttac
      841 ccgcacctgg gtaactacga tcatcaaagg cgtgccggtc ggagcgcaga tggcctggct
      901 atcttgcatc agcacaggca caccgcccgc cagcacgcgg gtcgcgcccg tcacccactg
      961 cgcggtaacg caggggccgt tgccgcccgg cggcacgaac gggcaacccg cgatgacata
     1021 ggggccggac tgcgtcacca ccggctgccc gcccaccagc acgcgcgggt tgggcacggt
     1081 cggttgggcc tggccgccgt gcgcgcatag caccgtcgcg ccgacgtgca ggagaaagcc
     1141 gggcatcaga tcacctccag agcgccgttg ttgagcgaga cgctggccgg ggtcagcttg
     1201 acgctggccg cgctgttgct gatctcgatg cccgacgcgt tgaacaccat tttcagcggc
     1261 gtgggcacca ccggcgggcc aacctctacc gtgaatccgc cggcgccggg caggtcgctc
     1321 agggtgagcg tgatgccgtc ggttttccag accttcatct ggggcagggc cggttgggcg
     1381 ggcacttcgc ccacgcccca gaagcagccg ctccagatgg ggtagtcgga gtcgccgccc
     1441 tcgaactcga cccagatgtt ggcgccgacc ggcgggacgg cgaagaagcc gacgcctgac
     1501 ccaccgtagg gtgcgcaggg catggcccaa ctgaggctgc cgtcgcccag cacagcgggc
     1561 acgctcacct gcacccgccc caattgcatc gggtcgatgt tgttggcgac cttgccgcgg
     1621 tacttgccga aaaactggct catgggatca ccgtgggcgt tgtcgacccg acgccctcgc
     1681 gggatagcgt aaagtgctgc tggtaggtgc ccctgcggac ggtgtggtcc acgctcttga
     1741 cgtaccagaa gccgtcgttc aggtagcccg cgccgcgcac gcccaccagg ccgcgcggtt
     1801 gcagcaggtt gccgtagcgg gtggcgtcca ggtcgcccac ggcgaccacg gcgtccaccg
     1861 aggcgtccat ggtgccctgg gcgcgggcga aggcctgcgc ggtgttcacg ccgctctcgc
     1921 ggaactggac gtggcgcagg ttggccgcgt tggcgaccca ggccgggaag gcggccagcg
     1981 gcgggcgcag gctggcgaag gtctgcaccg gcaggctctg gttggtggtg cggtcctgca
     2041 ccttgccctc gaccacggtg ggttccaggg cgttgttgcg cacgttgaag gtctgcacgt
     2101 tcgtctccgg ccccatgttg atggtcagcg ccgattgcgg cacccccgcg cggatcggcg
     2161 gcccccagta ggccgtgttg gtcagcggcg ccgggccggg cgtgatgtag aagacatagc
     2221 cgtagcgcgc ggccatctgt ttgagatatt gcagatcggt cgcctgctgg accggggtgc
     2281 gctcgatggg gatgggcacg tcgagcgcgg gcggcgggat gaccagcggg atgaggccgt
     2341 actgggcgta tttggcgatc agcttgagcg cgatgatcgt ctcgtcctgg gccgggtgct
     2401 cggcggactc ctctttgcgg tccatcatga cgctcacatc ctcgccgatg acagtcatag
     2461 tcgaggcgcc ggcctggtcg ctgggcgaga cctgcacatg ggtgatgatg ccgtccatga
     2521 gcacgcgcgg catgacgctg aaggtcacga cgatcaccac ccggttgaag ggcttgagca
     2581 gcgggttgct gaccagcgcg tagtcgagca ggtcggcggg gccggagcgc ccgatctgga
     2641 aggtgatctc gaagcccgat tggcggtcgt cgctgtgcga gacgcgcacg ctttgcagcg
     2701 cttcggtcaa ctggatcggg gccggcacgg cgatggtcgg gccgatcaac agggttaaat
     2761 gcacgcccaa tagcatggtg gcagagtcct tatccgatca ggatacgagc cgatacctca
     2821 gggttgcggg atggggacgc gcagcacgcg ccccacctgc gcggtcaaat cgggcgggtt
     2881 catggcgtcg ttggcgtcgg ccacgcgcca gaactgctcg gggtcgccca gcgtgcgggc
     2941 ggtgatcagg tccaggcggt cgccctgggc cacggtgatc tccaccagca gcggcatggc
     3001 cgcgccctgc ggcagaaacc gccgccggac gtaagcgacg gtgcggccgt ccggcatgag
     3061 caggccggcg gtttcgatat tgtagtagcg actggttggg tcgaacatcg tctgtcctcc
     3121 gctgggcggc gggtcgggtt gaaaccggcc cctacaccgg ccaaatcccg gtggccgg
//
LOCUS       DASUYC010001104         3588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1807959, whole genome shotgun sequence.
ACCESSION   DASUYC010001104 DASUYC010000000
VERSION     DASUYC010001104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1807959"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="VF276_18005"
     CDS             <1..655
                     /locus_tag="VF276_18005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6608799.1"
                     /translation="VRAAAERFAAGAPGVLLGAKGLAGLSAAVQALVAHGAQVVTGGQ
                     PLPGPGFHYANTILRATGAQFLADPAALQMEAYGPVSLLILAKSADAMRAMAGQVEGS
                     LTGTIYSHSGGHDDAAYDRLAPVLRPRVGRLLNDKMPTGVAVSAAMNHGGPYPATGHP
                     GFTGVGIPAAIRRFTALYSYDNVRSHRLPVELQDANPTGHLWRLVDGSWTQGDVPAG"
     gene            complement(716..985)
                     /locus_tag="VF276_18010"
     CDS             complement(716..985)
                     /locus_tag="VF276_18010"
                     /inference="COORDINATES: protein motif:HMM:NF044561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX6608800.1"
                     /translation="MDDVPPVRGFQTREARARYIAAAVAQAQYEMLPDDGGIYATVPG
                     LQGVWAQGKTRESAQAELAGVLEGWLTLSLERRRPIPLIRGWEAL"
     gene            complement(1000..1272)
                     /locus_tag="VF276_18015"
     CDS             complement(1000..1272)
                     /locus_tag="VF276_18015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608801.1"
                     /translation="MPQAQLTRGHRTHEIYIDGAPVTAAAGLDQDLVRLNTTTGRFYI
                     SRADYVTLEREWTPGEPMPVALIPPARAAEAPPRPLWSWIYPAPAP"
     gene            complement(1294..>3588)
                     /locus_tag="VF276_18020"
     CDS             complement(1294..>3588)
                     /locus_tag="VF276_18020"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6608802.1"
                     /translation="TIQKIEAGERRPSRHVAELLAEHLRVPADQHAAFLAFARGTPPA
                     PPPAPPGAPAAPPAPAPVLPAPSAPSRPVPLTRLIGRDATVGAVRRALRQGPARIVTL
                     VGPPGVGKTRLAVAVAEAAAADYPGGAPFIPLAALRDVALLPATIAGALGLGDRGGGA
                     PQAALLAALRDQEMLIVLDNFEQILPGAPNVVELLRACPGVRMLITSRAPLNVRGEQS
                     WPVAPLAVPDPAALPPLDALTEYPAVALFVERARMVQPAFTVRPDNAAAIAEICARLD
                     GLPLAIELAAARTRLLPPTVLLARLAQPLPLLTGGPRDLPARQQTLRDAIAWSYDLLT
                     PGEQCLFRRLGVFAGGWTLPAAEAIGNAVGDLPVPVLDAVATLLDHNLITAAPPAAGE
                     SRFTMLETIREYAIELLEAGPETATVHRWHAEYFLALAEAADPALSGADSAVVLARLA
                     AEQANIQAVFRWSIAAEGAATALRLGALYWRFCEMRGYLDEGRYWLEAVLAATDPAPV
                     AVRGLVLMGAGRVAWLQGDYAAARAHTEQSVAVFRALNDQANLPRALHSLALQLGALG
                     EPATARACYEESLALMRAQGNRAGVARLLNSLGIEARAQGDYARAVALFSESLALQRE
                     IDNKTSIGVLLANLGFATYDQGDLARAGALFRESLALLRDLGHRIAMIDGLAGLAAIA
                     TRRADPLRAARLFGAAEALAGAIGYTMEPADRAWYDRQVAALRATAPPAALAAAWAAG
                     RDLPLDQVLAEALATHDQPGPAVL"
BASE COUNT          490 a         1233 c         1402 g          463 t
ORIGIN      
        1 cgtgcgcgcg gcggcggagc gcttcgccgc gggggcgccg ggcgtgctgc tgggcgcgaa
       61 agggctggcc ggactcagcg ccgccgtcca ggcgctggta gcgcacggcg cgcaggtggt
      121 gacgggcggg cagccgctgc cggggccggg cttccactac gccaacacca tcctgcgcgc
      181 gaccggcgcg cagttcctgg ccgatccggc agccttgcag atggaagcct acgggccggt
      241 gagcctgctg atcctggcca agagcgcgga cgctatgcgg gcgatggcgg ggcaggtcga
      301 gggtagcctg accggcacga tctacagcca tagcggcggg cacgacgacg cggcctacga
      361 ccggctggcg ccggtcctgc gaccccgcgt ggggcggctc ttgaacgaca agatgccgac
      421 cggcgtggcg gtcagcgcgg cgatgaacca cggcgggccg tacccggcca ccgggcaccc
      481 cggcttcacg ggcgtcggca tcccggcggc gatccggcgc ttcacggcgc tctactccta
      541 cgacaacgtg cgctcgcacc ggctgccggt cgagctacag gacgcgaatc ccaccgggca
      601 cctctggcgg ctggtggacg gctcgtggac gcaaggggat gtgccggcgg gataggccag
      661 gcggcggcgg gctggctcaa ccggaccggc tgttatgcgg cacagagggg taagttcaga
      721 gcgcttccca tccgcgaatc aacggaatgg ggcgccggcg ttcgagtgag agggtcaacc
      781 acccctccag tacgcccgca agttccgctt gtgcgctctc gcgggttttc ccctgtgccc
      841 aaaccccttg caaccccggt accgtcgcat agatgccgcc gtcatccggt agcatctcgt
      901 actgtgcctg cgccacggct gcggcgatat agcgggcgcg ggcttcgcgg gtctgaaatc
      961 cgcgaaccgg gggaacatcg tccatcattg ccacctgcat taaggagcgg gggccgggta
     1021 gatccaggac cagagcgggc gcggcggggc ctcggcggcg cgggccgggg ggataagggc
     1081 gaccggcatc ggttcgccgg gggtccattc gcgttccagg gtgacgtaat ccgcgcggct
     1141 gatatagaag cgtcccgtcg tggtgttcag gcgcaccaga tcctggtcca gccccgcggc
     1201 ggcggtgacc ggcgcgccgt cgatgtagat ttcgtgcgtc cggtgcccgc gagtcaactg
     1261 cgcctgcggc atgtggtaac cctcctgcgc ccattatagc acggccgggc cgggttggtc
     1321 atgggtcgcc agggcctcgg cgagcacctg gtccagcggt aggtcgcggc ccgcggccca
     1381 ggccgccgcc aacgctgcgg gcggggcggt cgcgcgcaac gcggccacct ggcggtcata
     1441 ccaggcacgg tcggctggtt ccatggtgta gccgatggcg ccggccaggg cctcggcggc
     1501 gccgaagagg cgggcggcgc gcagcgggtc ggcccggcgg gtggcgatgg cggccagccc
     1561 ggccagcccg tcgatcatgg cgatgcggtg gcccaggtcg cgcagcaagg ccaggctctc
     1621 gcggaagagg gcccccgccc gcgccaggtc gccctggtcg taggtggcga agcccaggtt
     1681 cgccagcagc acgccgatac ttgtcttgtt gtcgatctcg cgctgcaagg ccaggctttc
     1741 gctgaagaga gctacggcgc gggcgtagtc gccctgggcg cgagcttcga tacccaggct
     1801 gttgagcagc cgggccaccc cggcccgatt gccctgggcg cgcatcaggg ccaggctttc
     1861 ctcgtagcag gcccgcgccg tcgcgggttc gcccagcgcc cccagttgca gcgccaggct
     1921 gtgcagcgcg cggggcaggt tggcctggtc gttcagcgcg cggaagacgg ccacgctctg
     1981 ctcggtgtgg gcgcgggccg cggcgtagtc tccttgcagc caggccacgc gcccggcccc
     2041 catcagcacc agcccgcgca cggccaccgg cgccgggtcg gtcgccgcta gcacggcctc
     2101 cagccagtag cggccttcgt ccaggtagcc gcgcatctcg cagaagcgcc agtagagcgc
     2161 gcccagccgc agcgccgtgg cggccccctc ggcggcgata gaccagcgga acacagcctg
     2221 gatgttggcc tgttccgccg ccaggcgggc cagcaccacc gccgagtccg cgccgctgag
     2281 cgccggatcg gcggcctcgg ccagggcgag aaaatactcg gcgtgccagc ggtggaccgt
     2341 cgccgtctcg gggccggcct ccagcagttc gatcgcatac tcgcggatcg tctccagcat
     2401 ggtgaagcgc gactcgcccg ccgcgggcgg ggccgccgtg atcaggttgt gatcgagcag
     2461 cgtggctacg gcatcgagca cgggtaccgg caggtcgccc acggcgttgc cgatggcctc
     2521 ggcggcgggc aaggtccagc cgccggcaaa tacgccgagc cgccggaaaa ggcactgttc
     2581 gccgggcgtg agcagatcat agctccaggc aatggcgtcg cgcagggttt gctggcgcgc
     2641 gggcagatcg cgcgggccgc cggtcagcag cggcaagggt tgggccagcc gggccagcag
     2701 gacggtgggc ggcaggagcc gcgtgcgcgc ggcggccaac tcaatcgcca gcggcagccc
     2761 gtcgagccgg gcgcagatct cggcgatggc cgccgcgttg tcggggcgca cggtgaaggc
     2821 cggttgcacc atgcgcgcgc gctcgacgaa cagggccacc gccgggtact cggtcagcgc
     2881 gtcgagcggg ggcagggcgg cgggatcggg cacggcgagc ggggccacgg gccaggattg
     2941 ctcgccgcgc acgttcagcg gggcgcggct ggtgatgagc atgcgcacgc cggggcaggc
     3001 gcgcagcagt tcgaccacgt tgggcgcgcc cggcaggatc tgctcgaaat tgtcgaggac
     3061 gatcagcatc tcctggtcgc gcagcgcggc cagcagcgcg gcctgcggcg cgccgccgcc
     3121 ccggtcgccc agacccagcg ccccagcgat ggtggccggg aggagcgcga catcgcgcaa
     3181 cgcggccagc gggataaacg gggcacctcc cgggtagtcg gcggcggcgg cttcggccac
     3241 ggcgacggcc aggcgcgttt tgcccacgcc cggcgggccg accagcgtga cgatgcgcgc
     3301 cgggccctgg cgcagcgccc ggcggacggc gccgaccgtc gcatcgcggc caatcaggcg
     3361 ggtcagcggc acggggcggc ttggcgcgga cggcgcgggc agcactgggg ccggcgcggg
     3421 tggggcggcg ggcgcgccgg ggggcgccgg cggcggggcg ggtggggtgc cgcgagcaaa
     3481 ggccaggaag gccgcgtgct ggtccgcggg gacgcgcagg tgctcggcga ggagctcggc
     3541 gacgtgccgc gaggggcgtc gctcgcctgc ttcgatcttc tggatggt
//
LOCUS       DASUYC010001105         2107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_2039015, whole genome shotgun sequence.
ACCESSION   DASUYC010001105 DASUYC010000000
VERSION     DASUYC010001105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2039015"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..1142
                     /locus_tag="VF276_18025"
     CDS             63..1142
                     /locus_tag="VF276_18025"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX6608803.1"
                     /translation="MTLDTRASLRSRPTRTHRRDAGPRPNWPVRVHHPLAAQMVLRTS
                     ANWDHDLSPATWAAEGSTAEFRLPLGAGHWAAKPCLRAGNRLIWAAGYDVALTGEPGG
                     QDLYPQFVAPRQGTVSDKLETPTHPATGRHYMRVYLPPDYDKQPDQRYPVVYMQDGAN
                     LFFPDESYSGHAWDVQHTMDLLIALGLIRPAIVVGIYPAARMAEYTRPGYEEYGRWLA
                     QELKPAVDRMYPTLADDPASAVVIGSSLGGVVSFYLAWQWPEVFGGAACLSPTFGWRD
                     NLFARVAREARRPGRFYVDSGGPGDNFQAARNMHAALERRGYRAGRDLYYQSFRQAGH
                     NERDWAARLHRPLQFLLGLPQPDAV"
     gene            complement(1165..1569)
                     /locus_tag="VF276_18030"
     CDS             complement(1165..1569)
                     /locus_tag="VF276_18030"
                     /inference="COORDINATES: protein motif:HMM:NF040320.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX6608804.1"
                     /translation="MAIETALRVQLLSRHGELARVARVLADAGVNLDAVAGIDTGQMS
                     AVELLVDQMEAAQQALNAAHISAASVPVAVAWLPNRPGSLARATAALAAAGISLRSVF
                     IIRTTGDQDQVAFGCAEAAQADAILGGLRETW"
     gene            complement(1690..1914)
                     /locus_tag="VF276_18035"
     CDS             complement(1690..1914)
                     /locus_tag="VF276_18035"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6608805.1"
                     /translation="METLTWHTHHDDMPDLHHEEYTVAELAEILGVGREFLLYAIRTG
                     ELKAEQINSHTIRIKREDSLDWLQRRGQFY"
BASE COUNT          324 a          768 c          674 g          341 t
ORIGIN      
        1 tctcaggtgg ccgccgaagg ctctcgtgta gggccgggcc tgtacggggg gggtccggcc
       61 cgatgacact cgatacccgc gcctccctgc gttcccgccc tacccgcacc catcgccgcg
      121 atgccggccc gcgcccaaac tggccggtgc gcgttcatca tcccctggcg gcgcagatgg
      181 tcctgcgcac gtccgccaat tgggaccacg acctgtcccc cgcgacctgg gccgccgagg
      241 gcagcaccgc cgagttccgg ctgccgctgg gcgccgggca ctgggccgcg aagccctgcc
      301 tgcgcgccgg caaccggctg atctgggccg ccgggtacga tgtggccctg accggcgaac
      361 cgggtgggca ggatctctac ccgcagttcg tggccccgcg ccagggcacg gtctccgata
      421 agctggagac gcccacgcac ccggccaccg ggcggcacta tatgcgcgtc tatctgccgc
      481 ccgactacga caagcagccc gaccagcgct acccggtagt ctacatgcaa gacggtgcca
      541 acctcttctt cccggacgag tcgtattccg gccacgcctg ggacgtgcag cacacgatgg
      601 acctgctgat cgccctcggc ctgatccgcc cggccatcgt cgtcggcatc tacccggcag
      661 cgcgcatggc cgagtatacc cggcccggct acgaggagta tggccgctgg ctggcacagg
      721 agttgaaacc ggcggtggac cgcatgtacc ccacgctggc cgatgacccg gcctcggcgg
      781 tcgtcatagg gtcctcactg ggcggcgtgg tctcgttcta cctggcctgg caatggcccg
      841 aggtgtttgg cggggcggcc tgcctctcgc ccacgttcgg ttggcgcgat aacctgtttg
      901 cccgcgtggc gcgggaagcg cgacggccag ggcgcttcta cgtggacagc ggcggccccg
      961 gcgacaactt ccaggcggcg cgcaatatgc acgccgcgct ggaacgccgc ggctaccggg
     1021 cgggccgcga tctctactac cagagcttcc gccaggccgg gcacaacgaa cgcgactggg
     1081 ccgcgcgcct gcaccgcccg ctgcagttcc tgctgggtct gccgcaaccc gatgccgtct
     1141 agcgcccggc ccagtcggcg ttacctacca ggtctcgcgg aggccgccca ggatcgcgtc
     1201 ggcctgggcg gcctcggcac acccgaaagc cacctgatcc tgatcccccg tcgtgcggat
     1261 aatgaagacc gaccgcagac tgattccggc ggccgccagc gctgcggtgg cgcgcgccag
     1321 cgagccgggg cggttaggca accacgccac tgccaccggc acgctcgccg cgctgatatg
     1381 cgccgcgttg agcgcctgct gcgccgcctc catctggtcc accagcagtt ccaccgcact
     1441 catctggccg gtatcgatgc ccgccaccgc gtccagattg acgcccgcgt cggccagcac
     1501 acgggccacc cgcgcgagtt cgccatggcg cgacagcaac tgcacgcgta gcgccgtttc
     1561 gatagccatc gtcacacctc ccgcgtcgcc gggcctaccg gcgcgtcttg atccataccg
     1621 ccgcagcata atctgtaccg gcaacgaggg atgatcgggt ttctgccggc cgggccgggc
     1681 catgccggct taatagaact gcccgcgccg ttgcagccag tccagcgaat cttcgcgctt
     1741 gatccggatc gtgtgcgaat tgatttgctc ggctttcaac tcgccggtcc ggatagcata
     1801 cagcaggaac tcacgcccca cacccaggat ctccgccaat tcggccaccg tgtactcctc
     1861 gtggtgcaga tcgggcatgt catcatggtg agtatgccag gttagggtct ccattgatcc
     1921 tctcctttca ggggccggca gtcgccgggg atccagcgtg cccggcaagt ggctccccat
     1981 ttcatcatag ccgaaagcgg ttggcgtagt ggtatatgcg cgtaacagat gggttaaaat
     2041 cgggtggcgg gcgtgaagaa acaccgcgcc gggccaccgc cccgcgcgat cccaggcaac
     2101 cttgggg
//
LOCUS       DASUYC010001106         4205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_653120,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001106 DASUYC010000000
VERSION     DASUYC010001106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653120"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            320..1987
                     /locus_tag="VF276_18040"
     CDS             320..1987
                     /locus_tag="VF276_18040"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HEX6608806.1"
                     /translation="MGYFHPARRMRLSRPRRGLLVLLLTLVLVAPLLPAPLTTPPPAA
                     AAAISIRWGFYVTYNPNSFASLQAHVDQLNYVSPWFYTVNGTGAITNSVQSNVNDLLR
                     SRGVKNVPMIRNNSTYQNFHNVLADAGMRAALIANLRTIVLQNNYDGITIDFEGILDA
                     DGPLLTTFMSSLYNDLKQYDKLVVMAVPAKTRDVNTGWAGPFNYRDLRPWADYFVIMA
                     YDQHYPGGAPGPIAPLPWLNDVANYATPILGAGKIIWGIDLYGYDWNTTANPRPPADP
                     RTWSETQALGQTYQGTFAYDTASQSPSLVYMQNNQRHEVWYQNKQSFDARLALVQQRN
                     YPGFAIWRLGQEDPGIWQSIAGLRSPCTPIPPFPNTPTRIYFPQTGHSLGGGFLEYWR
                     AHGGLPIYGYPLSEEFTEVSPTDGRPYTVQYFERNRFEWHPELAGTPYSIQLGLLGSQ
                     YTAGRQFPTVPPFPNTPDRIYFPQTQHSLGGGFYILWRQRGGLAQFGYPLSEEFTEVS
                     PTDGRPYTVQYFERARFEWHPEHLGTPSEFQLGLLGVWALERTGCRP"
     gene            2455..3630
                     /locus_tag="VF276_18045"
     CDS             2455..3630
                     /locus_tag="VF276_18045"
                     /inference="COORDINATES: protein motif:HMM:NF025124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6608807.1"
                     /translation="MPRPSVPLATADDNPAYESTLLRGAGIGDLDLEAVRAYLSALDA
                     EAGRSPADPAGAGPESGADVLERRLAALGLGAGTGADWHPTVAGLLLFGRAPHHALPQ
                     VQVKAARFRGSDVSGFIVDRAELSGAAGPLIEAVTQFVTRNMRVGGAIEGVYRRDVPE
                     YPIQAVREAVTNAVAHRDYSLTGQKVQVRMFDDRLEIESPGGLAGPVTLDTLEVRRYS
                     RNPRLAQAMYMLRLVEEMGTGIRRIKRALAELGSGPPSFVTDRASFLVRLPALPDAPA
                     PAESPAVATPAGPAAGAPAASREAPPVAPAAPPAPEISEEARRRSATWLRAGLSPRQA
                     AGLEHAFQSGRLTNREYRELHGELSDEAARLDLADLVERGYLMKIGSNRGTYYILRD"
     gene            complement(3771..4130)
                     /locus_tag="VF276_18050"
     CDS             complement(3771..4130)
                     /locus_tag="VF276_18050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016202155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6608808.1"
                     /translation="MEVVNLAEKFSRFQDYWQPKIVGELDDYHVKLVKVKGEFVWHQH
                     NAEDEMFLVVQGRLQIRLRDREVILNPGEMTIIPHGVEHMPVAAEETHLLLLERKATL
                     NTGNVQNERSVLAEEWI"
BASE COUNT          645 a         1516 c         1333 g          711 t
ORIGIN      
        1 cgggctgttc gtcttgttta tgaggcgcga tctcgctggc atccatctgc cggtcctcgg
       61 cgcgctaggg ctgtaaagcc tggtacgcga gttcatagca ataacagggc cgctttgggc
      121 cgctgcccag ttgcctggaa atcgaggcag cgcccgccca ttctggcgtt ggtattattc
      181 ggattgccgc ccgcgctcgc ctcgatcacc ggcgaggccg ccgggcctgc gcacccggat
      241 tcccgtccgg gctcgctccg taacgtattc cggcgaccga tccggatcgg tgctggcttc
      301 tcctgggaag gattgattca tgggttattt tcatcccgcg cggcggatgc gtctgagccg
      361 cccgcggcgc ggcctgcttg tcctgttgct gaccctggtg ctggtggcgc cgctgctgcc
      421 tgcgcccctg accacccccc ccccggcggc cgccgcggcc atctcgatcc gctggggctt
      481 ctatgtgacc tacaacccga actcgttcgc ctcgttgcag gcacacgtcg atcagttgaa
      541 ctatgtctcg ccctggttct acacggtgaa cggcaccggc gcgatcacca acagcgtgca
      601 atccaacgtc aacgatctgt tgcggtcgcg gggcgtcaag aacgtgccga tgatccgcaa
      661 caacagcacc taccagaact tccataacgt gctggccgat gcggggatgc gcgcggcgct
      721 catcgccaac ctgcgcacca tcgtgctgca aaacaactac gacggcatca ccatcgactt
      781 cgaggggatc ctcgacgccg acggcccctt gctgacgacc tttatgtcgt cgctctacaa
      841 tgacctgaaa caatacgaca agctggtggt catggccgtg cccgccaaga cccgcgacgt
      901 gaacacgggc tgggccggcc cgtttaacta ccgcgatctg cgcccctggg ccgactactt
      961 cgtcatcatg gcctatgacc agcactatcc cggcggcgcg cccggcccca tcgcgcccct
     1021 gccctggctg aacgacgtgg cgaactacgc cacgccgatc ctgggtgcgg gcaagatcat
     1081 ctggggcatc gacctctacg gctacgactg gaacaccacg gccaacccgc gcccgccggc
     1141 cgatccgcgc acctggagcg aaacccaggc gctgggccag acctaccagg gcaccttcgc
     1201 ctacgatacc gccagccagt cgccgtcgct ggtctatatg cagaacaacc agcgccacga
     1261 ggtctggtac cagaacaagc agagctttga cgcccgcctg gccctggtgc agcagcgcaa
     1321 ctatcccggc ttcgccatct ggcggctggg gcaggaagat cccggcatct ggcagagcat
     1381 cgccgggctg cgcagcccct gcacgccgat cccgcccttc cccaacacgc ccacgcggat
     1441 ctacttcccg cagacggggc acagcctggg cggggggttc ctggagtact ggcgggcgca
     1501 cggcggcctg cccatctacg gctatccgct ctccgaggag ttcaccgagg tcagcccgac
     1561 cgacggccgc ccgtacacgg tgcaatactt cgagcgcaac cgcttcgagt ggcacccgga
     1621 gttggccggt acaccctatt cgatccaact gggtttgctg ggtagccagt acaccgcagg
     1681 ccgccagttc ccgacggtgc cgccgttccc caacacgccc gaccggatct atttcccgca
     1741 aacgcagcac agcctgggcg gtgggttcta catcctctgg cggcagcggg gtggcctggc
     1801 ccagttcggc tatccgctct ccgaggagtt caccgaggtc agcccgaccg acgggcgccc
     1861 gtacacggtg cagtatttcg agcgagcgcg cttcgagtgg cacccggaac acctgggcac
     1921 gccgagcgag ttccaattgg gcctgctggg cgtctgggcc ctggagcgga ccggctgccg
     1981 gccctaaccc ggcacgcgcg gcggccggtc tgagcgcaag tgcgttcgcg ccgtttgtgc
     2041 tcaggccggg cgcgcccctc acgatggcct gcgccttgct gaggctggtt gcgatctgcg
     2101 gcgaagcgat tcacctcgat cagcgggtgg caccgatagc ggtataatcc gcccgcgcca
     2161 ctctccgtct gcgcacggac cttttgtacg cgcgttggct tcgggttcgc ggccaggagt
     2221 tggcccggcg cgaccctcag gccagccccg gtgtgaccgg cctgcctcta ttgccgtcat
     2281 attctagagc gcttcccgcg gagcgcgccg tgtcccggcg cctggccccg gcccgcggta
     2341 aaagccggcg ccgaaacgga ccggcgactc ccgccatata atgtggtata atcccttgcg
     2401 ctttgcgccg gggccattcc ctcgatccca tcacaggagt gattgttatt tcatatgccg
     2461 cgcccatctg tgccccttgc aaccgcagac gacaaccccg cgtacgagtc cacgctgctg
     2521 cgcggcgccg ggatcggcga tctcgacctg gaggcggtgc gcgcctatct gagcgcgctc
     2581 gacgccgagg ccgggcgctc gcccgccgac cccgccggcg ccgggccgga gagcggggcc
     2641 gacgtgctgg agcgccggtt ggcggccctg ggcctgggcg cgggcacggg tgccgattgg
     2701 caccccacag tggccggcct gctgctgttc gggcgcgcgc cccatcacgc gctgccccag
     2761 gtgcaagtca aagccgcccg cttccggggc agcgatgtct ccggcttcat cgtggaccgc
     2821 gccgagctga gcggcgccgc ggggccgctc atcgaagcgg tcacgcagtt cgtgacgcgc
     2881 aacatgcgcg tgggcggggc catcgagggc gtctaccgcc gtgatgtgcc cgagtacccc
     2941 atccaggccg tgcgtgaggc cgtgaccaac gccgtggccc accgcgacta cagcctgacc
     3001 gggcagaagg tccaggtgcg catgttcgac gaccgcctgg agatcgagag tccgggcggg
     3061 ctggccggcc cggtcacgct ggacacgctg gaggtgcgcc gctactcgcg caacccgcgc
     3121 ctggcccagg ccatgtatat gctgcggctg gtcgaggaga tgggcacggg catccgccgc
     3181 atcaagcgcg cgctggccga actggggtcg gggccgccca gtttcgtgac cgaccgggca
     3241 tcgttcctgg tgcggctgcc cgcgctgccc gatgcccccg cccccgccga gtcgcccgcc
     3301 gtggcgacgc ccgccgggcc ggctgccggg gccccggctg cctctagaga ggcaccgccg
     3361 gtcgcgcctg ccgccccgcc cgcgccggaa atcagcgagg aagcgcgccg ccgcagcgcg
     3421 acctggctgc gcgccgggct cagcccgcgc caggccgccg gtctcgaaca cgccttccaa
     3481 tcaggccgcc tgaccaaccg cgagtaccgc gaactccacg gcgagctcag cgacgaagcc
     3541 gcgcgcctgg acctcgcgga cctggtcgag cggggctacc tcatgaagat cggctccaac
     3601 cgcggcacgt actatattct gcgcgattag ccgtcagcgg tcagcggtcc gcggtccgct
     3661 atcagccgtc agtcgtcagc tatatacctg cgacgccact gcaccgtgtg cctgcggcac
     3721 cgctgtggga tcagcctgat agctgacggc tgacggctga ccgctaacgg ctagatccac
     3781 tcctcggcga ggacggagcg ctcgttctgc acgttgccgg tgttcagcgt ggctttgcgc
     3841 tccagcagca gcagatgggt ctcctcggcg gcgacgggca tatgctcgac gccgtgcggg
     3901 atgatcgtca tttcgcctgg gttgaggatg acctccctgt cgcgcaggcg aatctggagc
     3961 cggccctgca cgacaaggaa catttcgtct tccgcgttgt gctggtgcca gacaaactcg
     4021 cccttgacct tgaccagctt gacgtggtaa tcgtccagtt ccccgacgat cttcggctgc
     4081 cagtagtcct ggaaccggct gaacttctcg gccagattga ccacttccac cgcgccctcc
     4141 tttcgctcac ccaaccggcg cgcccagttc ctgcaacacc gtggccgtat cgaccacggt
     4201 ggcga
//
LOCUS       DASUYC010001107         3180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_904482,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001107 DASUYC010000000
VERSION     DASUYC010001107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904482"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1037
                     /locus_tag="VF276_18055"
     CDS             <1..1037
                     /locus_tag="VF276_18055"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX6608809.1"
                     /translation="DRVFGSGGKRLRPALAILATQAGPEPVETRRVVALGAAMETLHT
                     ASLVHDDLIDNSYVRRGLPTLNAWMSPAATVLAGDYLFARAAVLITETEHVQVIARFA
                     DSLRQLCDGELRQMFRNAFTSMPGLAAGLGLELATSENAPVALAERTVADDLALLPSD
                     AEYERRITGKTAALFATSAQTGAMLAGAPPAQVAALHGYGLALGRAFQIVDDVLDFTS
                     TEAELGKPVGSDLRQGHLTLPVTRFAQAHPDEWHSWIGRIPQLVKGSDYIFGQAPEGS
                     NKHSSTPEHEQAISELIARVAGSEGVRLALQRAAEYARAAQALLQAVPESAARTLLYN
                     VAEYVVQRSY"
     gene            complement(1169..1426)
                     /locus_tag="VF276_18060"
     CDS             complement(1169..1426)
                     /locus_tag="VF276_18060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608810.1"
                     /translation="MIQELVRDIYESASMDWATSEKVAYTMLQFLERKLPPDDYAHIQ
                     KYILGYHTYTLPPSTAYVGYTETPPSQADTGRQLGSPAAPR"
     gene            1828..2874
                     /locus_tag="VF276_18065"
     CDS             1828..2874
                     /locus_tag="VF276_18065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608811.1"
                     /translation="MTTSDQRGGPQGVHLVGSVPLENAEQVFRTANDILGSRLRRIPD
                     GETGVRTNWIGWQAGVLMQHPLFERVPTDSEYSPLPHFRLRAPTPAEDIRFENLGYAD
                     AARASWATFQRLQQASDLPAGTRFQVSLPTAVATVAFFVTPADQAATEPGYEARLLEE
                     VREITETVPHDQLAIQWDVAVEFGILEGVFPTGLQADEAGLIARLVRLGEAVPADVEL
                     GYHLCYGDAGHQHFTQPADTARLVAIANGIAAGLQRPLNWIHLPVPRDRSDDAYFAPL
                     RNLHLHPETELYLGLVHYTDGREGTRRRIAAAQRTVPVFGVGTECGMGRRPAETVREL
                     MQIHADLATPVRKE"
BASE COUNT          514 a         1069 c         1129 g          468 t
ORIGIN      
        1 tggaccgggt gttcggcagc ggcggcaagc ggctgcgccc ggcgctggcg atcctggcga
       61 cgcaggccgg cccggagcca gtggaaaccc ggcgcgtggt ggcgctcggc gcggcgatgg
      121 agacgctgca cacggcgagc ctggtccacg acgacctgat cgacaactcg tatgtccggc
      181 gcggcctgcc caccctcaac gcctggatga gcccggcggc gacggtgctc gccggagact
      241 acctcttcgc ccgcgccgct gtgctgatca ccgagaccga gcatgtgcag gtgatcgccc
      301 gcttcgccga tagcctgcgc cagttgtgcg acggcgaact gcggcagatg ttccgcaacg
      361 ctttcacctc gatgccgggg ctcgccgccg ggttggggct ggaactggcg accagcgaga
      421 acgcgccggt ggccctggcc gagcgcaccg tggccgacga cctggcgctg ctgcccagcg
      481 acgccgagta cgagcggcgc atcaccggca aaacggcggc cttattcgcc acgtcggcgc
      541 agacaggcgc catgctggcc ggggcaccgc ccgcccaggt cgcggcgctg cacggctacg
      601 ggctggccct gggccgcgcg ttccagatcg tggacgacgt gctcgacttc accagcacgg
      661 aggccgaact gggcaagccg gtgggcagcg acctgcgcca gggccacctg accctgccgg
      721 tgacgcgctt cgcgcaggcc caccccgacg agtggcactc ctggattggg cgcatcccgc
      781 aactggtcaa gggcagcgat tacatcttcg ggcaggcgcc cgaggggtcc aacaagcaca
      841 gctccacgcc cgagcacgag caggcgatca gcgagttgat cgcgcgcgtc gccgggtcgg
      901 agggcgtgcg gctggccttg caacgggcgg cggagtatgc ccgcgcagcg caagccctgc
      961 tgcaagcggt acccgagagt gcggcgcgca cgctgctgta caacgtcgcc gagtacgtcg
     1021 tccagcgatc ctattagcag tccgccaggc cgcaacaggc cggtttgaaa ccggccccta
     1081 caacggccgt gccggccggg cgcatatagt atcagggcat gaagaagccc ccgcgaccgt
     1141 agtggtcgcg ggggctttgt gcgggcggct agcgcggggc cgccgggctg cccaactggc
     1201 ggccggtgtc ggcctgcgag ggcggggtct cggtgtagcc gacgtaggcg gtcgacggcg
     1261 gcagggtgta ggtgtggtag cccaggatgt atttctggat gtgggcataa tcatccgggg
     1321 gcagtttgcg ctccaggaac tgcaacatgg tgtaagccac cttctcggac gtggcccaat
     1381 ccatcgacgc cgattcgtaa atatcgcgaa cgagttcttg aatcatctac aggctcctat
     1441 acgactgggc cgtgacccag cttcctccag cggatggcgc gggccgccgt gccgccggcc
     1501 tccgtgcgcc ggcgccgcat ccgcccggcc atcgcggtcc tcgcttatac tatcgtaaac
     1561 gtgcgggggc gtcaagtgtg gcgcgggtgc gtgaattcgg cgctaggcgg cagcgaatcg
     1621 ttgcccggtt agggtcccgc gctccggctt cttcaagcaa gatctctgcg ttcactgcta
     1681 atcttttggt cttccccgga tccgtgttat aatgaccggc gcgagggtag gcaagcgcat
     1741 ccccgggggg gcgcatgtcc ccaccgcagg ccaaccacgg acaaggcgcg caaccaccgg
     1801 gctacgcgca ggaggaggag acagaccatg accacaagcg atcagcgcgg cggcccacag
     1861 ggagtccatc tcgtggggag cgtgccgctg gaaaacgcgg agcaggtgtt ccgcacggcg
     1921 aacgacatcc tggggagccg cctgcggcga ataccggacg gcgagacggg cgtgcgcacc
     1981 aactggatcg ggtggcaggc cggggtgctg atgcaacacc cgttgtttga acgggtgccc
     2041 accgacagcg agtactcgcc cttgccccat ttccggctgc gcgcgcccac cccggcggag
     2101 gacatccggt tcgagaacct gggctatgcg gacgcggcca gggcctcctg ggccacgttc
     2161 cagcgcttgc agcaggccag cgacctgccg gcgggcacaa ggttccaggt gagcttgccc
     2221 acggccgtcg ccacggtggc ctttttcgtg acgcccgcgg accaggcggc gacggagccg
     2281 ggctatgagg cgcggctgct ggaggaagtg cgcgagatta ccgagaccgt gccgcacgat
     2341 caactcgcca tccagtggga tgtggccgtg gagttcggta tcctggaagg cgtgttcccc
     2401 accggcctcc aggcggatga agccggcctg atcgcgcgcc tggtgcgcct gggcgaagcg
     2461 gtgcccgccg atgtcgaact cggctaccat ctttgctacg gcgatgcggg ccaccagcac
     2521 ttcacgcaac ccgccgacac cgcccggctg gtcgctatcg cgaacggcat cgccgccggc
     2581 ctccagcggc cgctcaactg gatccacctg ccggtgccgc gcgaccgcag cgacgacgcc
     2641 tacttcgcgc ccctgcgcaa tctgcacctg caccccgaga cggaattgta cctcgggctg
     2701 gtgcattaca ccgacgggcg ggagggcacg cgccgccgca tcgccgccgc gcaacggacg
     2761 gtgccggtct tcggcgtcgg gacggagtgc ggcatggggc gccggcccgc ggagacggtc
     2821 cgcgagttga tgcagatcca cgccgatctg gccacacccg tgcgcaagga gtagccgggg
     2881 ccaggatgcc cggcccggct gccctatgca aagacctctc ccggttgcgg gagaggtctt
     2941 ttacagccgg cgctagacac atggcggcaa gcctgctata gtgaatcgac caggatggcg
     3001 gggcgcaccg tgcgcctcac gcgccggcac gaggacatgc cgtgagcgat aacgatgatc
     3061 tgattgggcg ccaggccgaa caggtggcgc tggaccaggc actggccgcg acccggcagg
     3121 gcagcggcgg catcgtgctg ctggccggcg aggcgggcgt gggcaagacg cggctgctgg
//
LOCUS       DASUYC010001108         4312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_422439,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001108 DASUYC010000000
VERSION     DASUYC010001108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422439"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="VF276_18070"
     CDS             <1..524
                     /locus_tag="VF276_18070"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6608812.1"
                     /translation="LLAHLRPPAVGEGDHCPFQELRPADLHGPPDGGPPARRATLDDM
                     DPLIDFYIRGFYSLARLPTRAAWHARLSEQIIHRTLYLIEQDGIVVSAAMSSAETPQA
                     AMIGGVATVKEYRNRGLSARCVCALCADLFARGIEQIGLFYLPANKPAARVYDKLGFR
                     PAGEWWLQRLYYW"
     gene            699..1403
                     /locus_tag="VF276_18075"
     CDS             699..1403
                     /locus_tag="VF276_18075"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010368273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6608813.1"
                     /translation="MRDGVPEQTLREMRDYYRARAAEYDEWFYRRGRYDRGPEANARW
                     FADAEEVFAAFAALHLTGDVLELAPGTGIWTERLVRTARTITAVDASPEMVTINRAKV
                     ASARVTYVLADLFAWHPDRTYDAVVFGFWLSHVPDERLDAFWQTVAAALRPGGTVFFV
                     DSRREQTSTAADHQLPDASSQVMTRRLNNGAEYQIVKNFPTPAAIEARCAAAGLTFTV
                     RETPTYFQYGIGTRRA"
     gene            complement(1478..3442)
                     /locus_tag="VF276_18080"
     CDS             complement(1478..3442)
                     /locus_tag="VF276_18080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608814.1"
                     /translation="MKRNHNAARLFGLPLLMLLVSGLLLPAGPAPVARAQAPAVLPFP
                     PVTVANAGPAMSSGPFFGALPVNGDWIAYTISNLGCGHCGTYVHTIVLRDTVDARQFT
                     IHAATYRDFNANDVVFGARDIQFAGPYLLWRQPTRPANPTYLPQYQAGDFDCSLCLFD
                     VRTGQARQAANLAALDTSDAGAVRPVALDAGGSGRVLVLVETQSAASLYLAHLDSADV
                     RAIPAALRPGNLISAVLSGATIVWSSTGDHQTFGLYAYHEADNSVTELAAGPQAFTNL
                     QTNGAAIFWNTASLVSRRDMTTGVVQHLDGVGVDFQVAGDLIAWTRETGQQAPVLLTV
                     RNLTSGQVLQQQEVGQAAIAGGWTTIGLGAFGAGKIVLVANDFAPEFGPPANQRILLG
                     WSTTPDPAFARVWARADAPVAAGQAPRSWLWGPQPRFLAEEAYAEGPGGRHLVEYYDK
                     SRMEVNHPADNSNDPYYVTNGLLAVEMVSGEIQTGDTSRIRASVPCTIPVVGDPRKDN
                     PVAPGYATFAAVASLHGEHQAASRIGAPVDDAIDVDGVVSKDAPHGALASYAAFTPQT
                     GHNIPNVFWTYLQGMQGTYGFDWTFVLGYPITEGYWTQMRVGGKDYPVLVQIYQRRVL
                     TYVPDFAPVWQIQQGNVGQHYLEWRYTLNQ"
     gene            3679..4254
                     /locus_tag="VF276_18085"
     CDS             3679..4254
                     /locus_tag="VF276_18085"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011322363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HEX6608815.1"
                     /translation="METLPQDAALIIIDVQQGLDDPKYGPRNNPQAEENMARLLAAWR
                     ETGRPIYHIQHMSREPQSPLRPGLPGNAIKPMVQPQGDEPVITKDVNSAFIGTDLEAR
                     LRRAGQQTVVLIGLTTEHCVSTTARMAGNLGFTTYVVSDATATFDMRGPDGTFYPAET
                     VHGVSLASLHGEFATVLDTATVLQELSAPVE"
BASE COUNT          712 a         1447 c         1473 g          680 t
ORIGIN      
        1 cgttgctggc ccacctgcgc cctcccgccg tgggcgaggg cgaccactgc cccttccagg
       61 aattgcgccc cgccgatctg cacgggccgc cggacggtgg cccgcccgcc cgtcgcgcca
      121 cgctcgacga tatggatccg ctgatcgact tctatatccg cggcttctat agcctggccc
      181 ggctgcccac gcgcgcggcc tggcatgccc gcctgagcga gcagatcatt caccgcacgc
      241 tctacttgat cgaacaggac ggcatcgtcg tctcggcggc gatgagcagc gccgagacgc
      301 cgcaggcggc catgatcggc ggcgtggcga cggtgaagga gtatcgcaac cgcgggctgt
      361 ccgcgcgctg cgtgtgtgcc ctctgcgccg atctgttcgc tcgcggcatc gagcagattg
      421 gcctctttta cctgcccgcc aacaagcccg ccgcgcgggt ctacgacaag ctcggcttcc
      481 ggccggcggg cgaatggtgg ttgcagcgcc tgtattactg gtaatcagcc acaccgattg
      541 tttctgtagg ggccggtttg aaaccggccc gcctgtctgt tgtgcgctgt aggggccggt
      601 ttcaaaccgg cccgcctgcc tgcggcgcgc tgtaggggcc ggtttgaaac cggcccaggc
      661 agccgccggc gttactaaag ggggtacggg gagtagcgat gcgagacggg gtgcccgagc
      721 aaacgctgcg cgaaatgcgc gactattacc gagctcgcgc cgccgagtac gacgagtggt
      781 tctaccggcg gggccgctac gaccgcggcc cggaagccaa cgcccgctgg ttcgccgacg
      841 ccgaggaggt ctttgcggcg ttcgccgccc tgcatctcac cggcgacgtg ctcgaactgg
      901 cccccggcac cggcatctgg acagagcggc tggtgcggac cgcacggacg atcacggcgg
      961 tcgatgcctc gcccgaaatg gtgaccatca accgggcgaa ggtggcgagc gcccgcgtta
     1021 cctacgtgct cgccgacctg ttcgcctggc acccggaccg cacctatgac gcggtagtct
     1081 tcggcttctg gctgtcccac gtgcccgacg aacggctgga cgccttctgg cagacggtcg
     1141 cggcggcctt gcgacccggc ggcacggtct tcttcgtgga cagccgccgc gagcagacga
     1201 gcaccgccgc cgatcatcag ttgccggacg caagcagcca ggtcatgacc cggcgactga
     1261 acaatggcgc cgagtaccag atcgtcaaga acttccccac ccccgccgcg atcgaggcac
     1321 gctgcgccgc cgccgggttg accttcaccg tgcgcgaaac gcccacctac ttccagtacg
     1381 gcatcggcac gcggcgggca taactcggca cacaaagaca caaaaaggga gcgcgacccg
     1441 gctgggtcgc gctccctgct gttggttgct gccgcgccta ttggttcagc gtgtaccgcc
     1501 attccaggta gtgctggccg acgttgccct gctggatctg ccagaccggg gcgaagtcgg
     1561 gcacataggt cagcacgcgg cgctgataga tctgcaccag caccgggtag tccttgccgc
     1621 ccacgcgcat ctgcgtccag tagccttcgg tgatcgggta gcccagcacg aaggtccagt
     1681 cgaagccgta ggtgccctgc atgccttgca gataggtcca gaagacgttc gggatattgt
     1741 gcccggtctg cggggtaaag gcggcatagg acgccagcgc gccgtgcggc gcgtccttgc
     1801 tcaccacgcc gtccacgtcg atggcgtcgt ccaccggggc gccgatgcgg ctggcggcct
     1861 ggtgctcgcc gtgcaggctg gcgacggcgg cgaaggtggc gtagccgggc gccacggggt
     1921 tgtccttgcg cgggtcgccc accaccggga tggtgcaggg cacgctcgcc cggattcggg
     1981 acgtgtcgcc ggtctggatc tcgccgctga ccatctccac ggccagcagt ccgttggtca
     2041 catagtaagg atcattggag ttgtccgccg ggtggttgac ttccatgcgg ctcttgtcgt
     2101 agtactccac cagatgccgg ccgcccggcc cctcggcata ggcttcctca gccaggaagc
     2161 gcggctgtgg tccccagagc cacgagcgcg gcgcctggcc cgccgccacc ggcgcgtcgg
     2221 ccctggccca gacgcgggca aaagccggat cgggcgtcgt tgaccagccc aggaggatgc
     2281 gctggttcgc gggcggaccg aactccggcg cgaagtcgtt cgccacgagc acgatcttgc
     2341 ccgcgccgaa tgcgcccagg ccgatggtgg tccaaccgcc cgcgatggcc gcctgcccga
     2401 cctcttgttg ctgcagcacc tggccgctgg tcaggttgcg caccgtgagc aggacaggtg
     2461 cctgctggcc ggtctccctg gtccaggcga tcaggtcgcc cgccacctgg aagtctacgc
     2521 ccacgccgtc caggtgctgc accaccccgg tcgtcatgtc gcggcgtgag acgaggctcg
     2581 ccgtgttcca gaagatagcc gctccgttcg tctgtagatt agtgaatgct tgcggccccg
     2641 ccgccagttc ggtgacgctg ttatcggcct catggtaggc atagaggcca aaggtctggt
     2701 gatcgccggt gctggaccag acaatggtgg cgccggaaag cacggcggag atgagattac
     2761 cgggccgcag cgccgcgggg atggcgcgga catccgccga gtcgaggtgc gccaggtaca
     2821 gcgaggctgc gctctgggtc tccaccagga ccagcacgcg cccgctgccg ccggcatcca
     2881 gggccaccgg gcgcaccgcg cccgcgtccg aggtatcgag cgccgccagg ttcgcagcct
     2941 gccgggcctg gccggtgcgc acgtcgaaca ggcagagcga gcaatcgaag tctcccgcct
     3001 ggtactgcgg aaggtaggtg ggattcgccg ggcgggtggg ctgccgccac agcaggtagg
     3061 ggccggcgaa ctggatgtcc cgtgcgccga agaccacgtc gttggcgttg aaatcccggt
     3121 aggtggcggc gtggatggtg aactgccggg catctacggt gtcgcgcagg acgatggtgt
     3181 ggacataggt gccgcaatgg ccgcagccga gattactgat cgtgtaggcg atccaatccc
     3241 cgttgaccgg gagcgcgccg aaaaaggggc cgctggacat agccggcccg gcattggcga
     3301 cggtcacggg ggggaagggc agcacggcgg gcgcctgcgc tcgcgcgacg ggggcaggcc
     3361 cggcaggcaa taacaggccg gacaccagca acattaacaa cggcaggcca aataagcgcg
     3421 ccgcattgtg atttcgtttc atcgcaaact cctgtttggt tgggcaggct gtccaaccct
     3481 gcccttatac ggacccggaa agctcattcg ctagtaacac gaacaggcgg cgggctggtt
     3541 gcgcggggcc gccgggccgc gggtgtaagc ttgcctaccg cgcgagggtg cctcactggt
     3601 ataatggtgt ccagcaccat cgcgggtaac gcggcaaccg ggcacggcag ccaatgggct
     3661 gcgcagaaag ggaatcccat ggagacactt ccgcaggacg cggcgctgat cattatcgac
     3721 gtgcagcaag gcctggacga ccccaaatac ggcccgcgca acaatcccca ggccgaggag
     3781 aacatggccc ggctgctggc ggcctggcgg gagaccgggc gcccgatcta ccacatccag
     3841 cacatgtcgc gtgagccgca gtcgccgctg cgccccggcc tgccgggcaa cgccatcaag
     3901 ccgatggtcc agccgcaggg cgacgagccg gtcatcacca aggacgtgaa cagcgccttc
     3961 atcggcaccg acctggaagc gcggttgcgc cgggcgggcc agcagaccgt ggtgctcatc
     4021 ggcctgacca ccgagcactg cgtctccacc acggcgcgca tggcgggcaa cctgggcttc
     4081 acgacttatg tggtcagtga cgccacggcc accttcgaca tgcgcggccc cgacggcacg
     4141 ttctaccccg ccgagacggt gcatggcgtc tccctcgcca gcctgcacgg agagttcgcg
     4201 accgtgctcg atacggcaac tgtgctgcag gaactgagcg ccccggtcga gtaagcgaaa
     4261 ggagtgcgcg gtggaggcgg taaatctggc cgagaagttc agccggttcg ag
//
LOCUS       DASUYC010001109         3324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1578252, whole genome shotgun sequence.
ACCESSION   DASUYC010001109 DASUYC010000000
VERSION     DASUYC010001109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1578252"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /locus_tag="VF276_18090"
     CDS             <1..1003
                     /locus_tag="VF276_18090"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX6608816.1"
                     /translation="VVACPGRLIDHISESTIALARVETLVLDEADRMFDMGFLPDIRR
                     ILKHLPAERQTLLFSATMPADIRRLAQEVLRNPITVQVGTMEPVKAVSHALYPVATHL
                     KTPLLLALLRQTDTESVLVFTRTKYKAKRLGEQLVNAGFRGASLQGNLGQNRRQAALD
                     GFRDGTHQILVATDIAARGIDVSQISHVINYDMPDTVEAYTHRIGRTGRAAKTGDAFT
                     FVTDEDQDMVRAIERALGGAVPRHKLPGFDYSVPAPVRDTEFARPPRVPQPRRAKPAP
                     APAPAATPVPATPAPARRPAASPAPAPAPKRARPARAPRPGNAPGAPASRAHRHATG"
     gene            complement(1082..>3324)
                     /locus_tag="VF276_18095"
     CDS             complement(1082..>3324)
                     /locus_tag="VF276_18095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608817.1"
                     /translation="PLTVLMGYGVVAALAAHLLLAPGRVVARWLAPEARYLGRLLARP
                     AIWALVGVAVLGGVAAYQVRHSYDIDIGDATDQAYTRNFHERLVDDATGRTYRTLDAY
                     GYIVLTGVGGSAPYSVTLSLNPGAPDVGLTVILNGETFLQQRMAPGWQDYSFTIDAAH
                     PRALAARDLVIELRGRPARAVLVDRVRIGPQQGGFVAPTYSQLGYLGALVLLVYLLLG
                     RAFAPLPAEGVVVSDRDSDRRQAGLRPAPTPQMDRGGLIVRRGAPLPAGPLPWRPALG
                     GAALAALALVGALAAVHLPITEAAEHLVATGAAGYVLLVLAEPLAARLAPGTARGARL
                     AALLFAGAFLVRFGAMALPQTVIIDMPYHLKWMRELLAGNVDALTDPHGGLNEPPREW
                     GLAVFIPKSPLFYFLAAPLALLPGDLETGLDGLVCLLEASTVLFCYGLLARFAPRWGG
                     WQAGLWAGFAYAANPLGFRALSYGILPTILAQWLSLAFLALLLVWVQDRLRAPADGPP
                     VRRGAWTTGAGWGLLLVLLTAALIAFPTIAVFTSLVTGGVALVWLRARYRRVGWAVGA
                     LLAAAWIAAIALYYGVYIADLLTQTLPQMLAPHPATAGTPPPSTVHWTGPLDLLGWTL
                     GYLVSPLPLVMGLAGLVVLWRSVWGRGSQAGESPVLPERLLAPLTAFWTAILPVFIVA
                     NYRIDMIGKHLYYTMVPLSLGSGIFLWLLARRGGWPRRAAWALAAVLLLTALLFWADR
                     LVRAST"
BASE COUNT          528 a         1229 c         1180 g          387 t
ORIGIN      
        1 cgtcgtggcc tgccccggtc gcctgatcga ccacatcagc gagagtacca tcgcgttggc
       61 gcgggtcgaa acactggtgc tggacgaggc ggaccgcatg ttcgatatgg gcttcttgcc
      121 cgacatccgc cggattttga agcacctgcc cgccgagcgg caaacgctgc tgttctcggc
      181 caccatgccc gccgacatcc ggcgcctggc gcaagaggtg ctgcgcaacc cgatcacggt
      241 gcaggtcggc acgatggagc cggtcaaggc ggtctcgcac gcgctctacc cggtggcgac
      301 gcacctcaag acgccgctgt tgctggcgct gctgcgccag accgacacgg agtcggtgct
      361 ggtgtttacc cgcaccaagt acaaggccaa gcgcctgggc gagcagttgg tcaacgccgg
      421 cttccgcggc gcctcgctgc aaggcaacct gggccagaac cggcgccagg ccgcgctcga
      481 cggcttccgt gacggcacgc accagatcct cgtggcgacc gacatcgccg cgcgcggtat
      541 cgatgtgtcg cagatctcgc acgtcatcaa ctacgacatg cccgacaccg tggaagccta
      601 cacgcaccgc atcgggcgca ccgggcgcgc ggccaagacc ggcgatgcct tcaccttcgt
      661 caccgacgag gaccaggaca tggtgcgggc cattgagcgc gccctgggcg gggcggtgcc
      721 gcgccacaag ctgccgggct tcgactattc agtgccggcg cccgtgcgcg acaccgaatt
      781 tgcccgcccg ccgcgcgtgc cccagccgcg ccgggccaag cctgccccgg caccggcgcc
      841 tgcggccacc cctgtgcccg ccaccccggc gcccgctcgc cgcccggcgg ccagtccggc
      901 gcctgcgccc gcccccaagc gggctcgccc cgcccgcgcg ccgcgtccgg ggaacgcgcc
      961 cggcgcaccc gcgtcccgcg cccaccggca cgcgaccgga taagctaatt tgagctaagt
     1021 gttggaccgg ctcgctacag cgagccggtt tttctatatc tcatcactcc ggatagttcg
     1081 cttatgtgct ggcccgcacc agccggtcgg cccagaagag cagggcggtg agcaagagca
     1141 cggcggccag cgcccaggcg gcgcggcggg gccagccccc tcgacgggcc agcagccaga
     1201 ggaagattcc gctgcccagc gacaacggca ccatcgtgta gtagaggtgc ttgccgatca
     1261 tgtcgatgcg gtagttggcg acgatgaaga caggcaggat ggccgtccag aaggcggtga
     1321 gcggggccag caggcgctcc ggcagcaccg gggactcccc cgcctgcgac ccgcgccccc
     1381 acacgctgcg ccagagcacc accagcccgg ccagccccat gaccagcggc agcgggctga
     1441 ccaggtagcc cagcgtccag ccgagcaggt cgagcgggcc ggtccagtgc acggtgctgg
     1501 gcggcggggt gcccgccgtg gcggggtgcg gcgccagcat ctgcggcagg gtctgcgtca
     1561 gcaggtcggc gatgtagacg ccgtagtaca gggcgatggc cgcgatccag gcggcggcca
     1621 gcagcgcccc caccgcccag cccacccgcc ggtagcgcgc ccgcaaccag accagcgcca
     1681 ccccgcccgt cacgaggctg gtgaacacgg cgatggtggg gaaggcgatc agcgcggcgg
     1741 tgagcagcac cagcagcagg ccccacccgg ccccggtggt ccacgccccg cgccgcacgg
     1801 gcgggccatc tgccggcgcc cgcagccggt cctgcaccca gaccagcagc aaggccagga
     1861 acgccaggct gagccactgg gccaggatgg tcggcaggat gccgtaggag agggcgcgga
     1921 agccgagcgg gttggcggca taggcgaagc ccgcccatag ccccgcctgc catccgcccc
     1981 agcgcggcgc gaagcgggcg agcagcccgt agcagaacag caccgtgctc gcttccagca
     2041 ggcagaccag gccgtccagc cccgtctcca gatcgcccgg cagcagggcc agcggcgcgg
     2101 ccaggaagta gaacagcggc gacttgggga tgaagacggc gagcccccac tcgcggggtg
     2161 gttcgttgag gccgccatgc gggtcggtca gcgcgtccac gttgcccgcc agcagttcgc
     2221 gcatccactt caagtggtag ggcatgtcga tgatgaccgt ttgcggcagg gccatggcgc
     2281 cgaagcgcac caggaaagcc ccggcgaaca gcagtgcggc cagccgggcg ccccgcgcgg
     2341 tgcccggcgc cagccgcgcg gccagcggct cggccagcac cagcagcaca taccccgccg
     2401 ccccggtggc gaccaggtgc tcggcagctt cggtgatcgg caggtggacg gcggccaggg
     2461 cgccgaccag ggccagcgcc gccagcgccg cgccgcctag cgccgggcgc cagggcagag
     2521 gcccggcggg cagcggcgcg ccccggcgca caatcaggcc gccccggtcc atttgcggtg
     2581 taggggccgg tctcaaaccg gcctgccgcc tatcggaatc acggtcggag acgactaccc
     2641 cttcggcggg gagcggcgcg aacgcccggc ccagcagcag gtacaccagc agcaccagcg
     2701 cgcccaggta gccaagctgg ctgtaggtgg gcgcgacgaa gccgccctgt tgcggcccga
     2761 tgcgcacgcg gtcgaccaga acggcgcgcg ccgggcgccc gcgcagttcg atcaccaggt
     2821 cgcgggcggc cagggcgcgc gggtgggcgg cgtcgatggt gaagctgtag tcctgccagc
     2881 cgggcgccat gcgctgctgc aagaaagtct cgccgttcag gatcaccgtc agccccacgt
     2941 cgggagcgcc ggggttcagg ctcagggtga cgctgtaggg cgcgctgccg cccacgccgg
     3001 tcaggacgat gtagccgtag gcgtcgagcg tgcgataggt gcggcccgtg gcgtcgtcca
     3061 ccagccgctc gtggaagttg cgcgtgtagg cctgatccgt ggcgtcgccg atgtcgatgt
     3121 cgtagctgtg gcgcacctgg taggcggcca cgccgccgag cacggccacg cccaccagcg
     3181 cccagatggc gggccgggcc agcaggcggc ccaggtagcg cgcttcgggc gcaagccagc
     3241 gcgccaccac tcgccccggc gccagcagca ggtgcgcggc cagcgcggca accaccccgt
     3301 agcccatcag cacggtcagc ggca
//
LOCUS       DASUYC010001110         5746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_885898,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001110 DASUYC010000000
VERSION     DASUYC010001110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5746
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885898"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VF276_18100"
     CDS             complement(<1..617)
                     /locus_tag="VF276_18100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6608818.1"
                     /translation="MTIRLVIADDHVVVRRGLRSFLEVMPDVEIVGEAADGEEAVVLA
                     QQLRPDVVLMDLLMPRMDGIAATAAIRGQLADTEVIALTSVLEDAAVIGAVRAGAIGY
                     LLKDADGDELYRAIKDAVAGQVHLAPQAVARLLHEVRYPESPEPLSERETQVLKLVAQ
                     GHSNKEVAQQFSLSEKTIKTHMSNILSKLGLSSRTQAALYAVRIGL"
     gene            905..1312
                     /locus_tag="VF276_18105"
     CDS             905..1312
                     /locus_tag="VF276_18105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6608819.1"
                     /translation="MAAATEPVARTTVLIVDDQPAVLSAVRDWLAYETDLDIIGQAED
                     GVTALILAQELQPDIVLIDLKMPNMDGIATAEALREVAPQVRVVILTIYDDVASRARA
                     QAAGVAAFVAKHDSVEALPAAIRKVAAGRQECL"
     gene            1900..2667
                     /locus_tag="VF276_18110"
     CDS             1900..2667
                     /locus_tag="VF276_18110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6608820.1"
                     /translation="MGSQETVRGANPARARVLVVDDEANLVELVQKYLQLEGFAVLTA
                     ADGLTALEIVRSARPDLIVLDLLLPGLDGIEVCRRLRTFSDAYVLMLTARAEEIDKII
                     GLSVGADDYLTKPFSPRELVARVKAMLRRPRAGAAGAAPPAAPAPLIFDDLVIDPAAH
                     AVSRDDMPIRLTPREYDLLWTLAGYPGRVFTRDQLLTQVWGDAYYDEHVVDVHVNNLR
                     KKLDDDAAHPRYIETVRGVGYRFLGTRRQAQEDGGRV"
     gene            2651..3823
                     /locus_tag="VF276_18115"
     CDS             2651..3823
                     /locus_tag="VF276_18115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6608821.1"
                     /translation="MAAVFNFPWARASLRAKLLAAHLIVVAVGVVTVLLITELLAPPF
                     FDIHMASMIDHAGMMGSSGMMNGTAADAALRAAYLTAVTQAVLVAGTAATLAAVLVSL
                     FVSARIVGPVQGLVAATRRIAAGHYAERVPWTKLDREDELGQLAGSFNAMAAALETTE
                     RRRVELLGDVAHELRTPISTLEGYLEGLLDGVVEPSPETWARLHGEAGRLHRLVSDLQ
                     DLSRAESRQLALKLQAVDPAQITEIALARVRQPLADKGLELVTQIPAPLPAVRADLDR
                     AAQALTNLLANALRYTPAPGKVTLAIGRTPAGNEVLFQVADSGIGLAAEHLPHLFERF
                     YRVDKSRSRAAGGSGIGLTIAQAVVEAMGGRIWAESPGPGQGSTFSFTLPLAEGGE"
     gene            complement(3935..4609)
                     /locus_tag="VF276_18120"
     CDS             complement(3935..4609)
                     /locus_tag="VF276_18120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6608822.1"
                     /translation="MARILVIDDDPAVTSVLKRGLAYEGFGVDTAAAGAAGLALARER
                     YPDLVILDIMMPGLSGMEVLQRLRAADPQLPILLLTGKDAPADQVAGLAGGADDYVVK
                     PFTFEVLLARVNALLRRRASERPPVLRFADLSLDTGTRRAQRGHREIDLTSTEYDLLV
                     QFLEHPARALSKEFLIDRVWGYDFGGNNNIVEVYVKQLRQKLEAGGESRLIHTLRGTG
                     YVLRED"
     gene            complement(4760..5476)
                     /locus_tag="VF276_18125"
     CDS             complement(4760..5476)
                     /locus_tag="VF276_18125"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX6608823.1"
                     /translation="MEGQPLTLPVVMGAGLVDSLNPCAFALLMVFIATMLAMIQRRPD
                     RAGERAARGWLLTHGGVYVVGIFITYLALGLGLLSSLEGLKLVSSTHFASRLAAFFAL
                     GLGLLALQEVLLPEWGTRMTAHVDHVKLKGMVMKLSFPALLLAGFLVGLCTVPCAGSV
                     YLGVLALLSTQATFAQGLAYLVIYNLVFIAPLVIILGFSSSPLVYRRLARWQMHRRAV
                     LKLATSAAAITVGIFTLLIV"
     gene            complement(5493..>5746)
                     /locus_tag="VF276_18130"
     CDS             complement(5493..>5746)
                     /locus_tag="VF276_18130"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX6608824.1"
                     /translation="GTLRIPAGKPVTVRLVNNESPYHASGALHQFAVDALGIDVKLDA
                     KQSRVITLQSDQPGTYTYYCDVCCGGKKSPNMQGTLTFY"
BASE COUNT         1010 a         1864 c         1924 g          948 t
ORIGIN      
        1 agaccgatgc gtacggcgta aagagccgcc tgtgtgcggc tcgacaagcc caacttactc
       61 aggatgttgc tcatatgcgt cttaatagtc ttttcgctca aactgaactg ctgagcaacc
      121 tctttgttgg agtgaccctg cgccaccagt ttgagcacct gggtctcgcg ctcgctgagg
      181 ggctccggac tctccggata gcggacctcg tgcagcagcc gggcgaccgc ctgcggggcc
      241 aggtgcacct gaccggcgac ggcgtccttg atggcgcggt acaactcatc gccgtcggcg
      301 tccttcagca ggtagccgat ggcgcccgcc cgcaccgcgc caatcaccgc cgcgtcttcc
      361 agcacactgg tgagcgcgat tacttcggtg tcggctagtt gtccgcggat cgcggcggtg
      421 gccgcgatgc catccatccg gggcatgagc agatccatga gcaccacatc cgggcgcagt
      481 tgttgggcca acacgacggc ttcttcgcca tccgccgcct cgcccacgat ctcgacgtcg
      541 ggcatcactt caagaaagct acgcaagccc ctgcgcacta cgacgtgatc gtccgcaatc
      601 accagacgaa tggtcactgg ccgccgtcct ttctgggcta cgtacatcgg tgtagcgatc
      661 cctaatacca aaggcgggag aaacgttgtc ccctcgcccg ctgttaagca ccacgcggtg
      721 tgcgagaccc cggtcaggta gacccacctc actgctgtgc ggtgtgccgc ctattatatg
      781 agcaggaaac acaaccatgc aagcgggttc agggccagag gtgggaaacg ggcgtctcca
      841 ggaccggata tgggtctgtt agcgggtgat acccgccgtc cggtgccgcc aggagggttt
      901 tcacatggca gcggcaactg agccggtagc gagaacgaca gtgctcatcg tcgatgatca
      961 gcccgcggta ctgagcgcgg taagggattg gcttgcgtat gagaccgacc ttgatatcat
     1021 aggtcaggcg gaggacggcg tcaccgcgct gatcctggcg caggagctac agccggatat
     1081 tgtgctaatc gacctcaaga tgccgaacat ggatggcatc gccaccgcgg aggcactacg
     1141 ggaagttgcg ccccaggtgc gggtggtaat actaaccatc tacgacgacg tggcgagcag
     1201 agccagggcc caggcagccg gcgtcgccgc gtttgtagcc aagcacgact cggtagaagc
     1261 gctgccggcg gcgattcgga aagtcgccgc cggcaggcaa gagtgcctgt agcgccatgc
     1321 gccggcggtg gcgaacagca ccctacccag ccccgcctgg agtcgccggc ctgctacagc
     1381 gggcaaggat ccggatgcgc acgcgggccg cgggtcggat ctgcggcaca gcataggcta
     1441 gcagggtcat ggtttcttca tagctgcgca cgatctccgc acacggtggg catacgacga
     1501 tgtgccgatg aacggcctgc tgctcatccg agtccagtgc tccagccgcg gccgccggca
     1561 acgcgggggc gatagattta cagtccatgg cgatctcctt tccggaccgc cggttgtatt
     1621 aggtgccctg gtgccgcgac gggcgggcac aatagccggc agccgcatgc tgtctacctg
     1681 ccctgtctgg gggtcgcagc caaccggcat aaaccggtct cagcgtaagc atacgcctgc
     1741 cggctgaggc caagttaacg gatgctgagt aatgcctgag aatgtgaacc gctggaccgg
     1801 tgcgccgcaa gtacgcaaaa cccgccctga tcgctccatt tgcgtttggc ccctccccat
     1861 cttacaatga gtaagagccc gagagaggag caactgccga tgggatcgca agagacagtg
     1921 cgcggcgcca acccggcgag ggcgcgggtg ctggtggtag acgacgaagc caatctagtg
     1981 gagttggtgc agaagtatct gcagttggag ggctttgcgg tcctgaccgc tgccgacggg
     2041 ctaaccgccc tggagatcgt ccgctccgcc cggcccgacc tcatcgtcct cgatctgctg
     2101 ctgccgggcc tcgacggcat cgaagtgtgc cgccggctgc gcacgttctc ggacgcctat
     2161 gtgctgatgc tgaccgcacg cgccgaggag atcgacaaga tcatcggtct gtccgtcggc
     2221 gccgacgatt acctgaccaa gcccttcagc ccgcgggagc tggtggcgcg cgtcaaagcc
     2281 atgctccgcc ggccccgcgc tggggccgcg ggcgccgcgc cacccgcggc gcccgcaccc
     2341 ctgatcttcg acgatctggt gatcgacccg gctgcgcacg cggtatcacg cgatgacatg
     2401 cccatacggt tgacgccgcg cgagtacgac ctgttgtgga ccctggccgg gtatccgggg
     2461 cgggtcttca cccgcgacca gctcctgacc caggtttggg gcgatgcgta ctacgacgag
     2521 cacgtggtgg acgtgcatgt gaacaacctg cgcaagaagt tggacgacga tgccgcccac
     2581 ccgcggtaca tcgagacggt gcgcggggtc gggtatcgct tcctggggac gcgccggcag
     2641 gcgcaggagg atggcggccg tgtttaactt cccctgggcg cgcgccagtc tacgcgccaa
     2701 gttgctggcg gcccacctga tcgtcgtggc ggtgggtgtg gtcaccgtgc tgctcatcac
     2761 cgaactgctg gccccccctt tcttcgacat ccatatggcg agcatgatcg accatgccgg
     2821 gatgatgggg tcgagcggga tgatgaacgg gacggcagcc gatgccgcgc tccgcgcggc
     2881 gtatctgacc gcggtgaccc aggcggtgct ggtggcgggc acggcggcga cactcgcggc
     2941 ggtgctggtc agcctgttcg tgtccgcgcg catcgtgggg ccggtgcagg ggctggtggc
     3001 cgcgacgcgg cgcattgccg ccgggcacta cgccgagcgt gtgccgtgga ccaaactgga
     3061 ccgcgaagat gaactgggcc aactggccgg cagcttcaat gcgatggcgg cggccctgga
     3121 gaccaccgag cggcggcggg tggagttgct cggcgacgta gcccacgaac tgcgcacgcc
     3181 gatcagcacg ctggaaggct atctggaggg cttactcgac ggggtggttg agccgtcgcc
     3241 cgagacgtgg gcccggctgc atggcgaggc cgggcggttg catcggctgg tgagcgatct
     3301 gcaggatctc tcgcgggctg agtcgcggca actggcgctc aagctgcaag ccgtagaccc
     3361 ggcgcagatc accgagatcg cgctggcccg cgtccggcag ccgctggccg acaaggggct
     3421 ggagctggtc acccagatcc ctgccccgtt gccggcagtc cgcgccgatc tggaccgggc
     3481 ggcccaggcg ctcacgaatc tgctcgccaa cgccttgcgt tataccccgg cgcccggcaa
     3541 ggtcaccctg gcgattggcc ggacgccggc aggcaacgaa gtcctcttcc aggtcgccga
     3601 cagcggtatc ggcctggccg ccgagcacct gccgcatctc tttgagcggt tctaccgggt
     3661 ggacaaatcg cgcagccggg ccgcgggcgg gtcgggcatc ggcttaacca tcgcccaggc
     3721 ggtcgtcgag gcgatgggcg ggcgcatctg ggcggagagt cctgggccgg ggcagggcag
     3781 cactttctcg ttcacgctcc cgctggccga aggtggcgag tagcccagcc tgcccgccgc
     3841 tccgtacacg agcgggcacc tgggggcaaa gctatgggca gccacccctg cctggcctgc
     3901 gcggaggccc gtggccggag ggaatcacga cgggctagtc ttcgcgcagc acataaccgg
     3961 tgccgcgcag ggtatggatg aggcgggact cgcccccggc ctccagtttc tggcgcaatt
     4021 gcttgacgta cacctcgaca atattgttgt tgccgccgaa atcgtacccc cagacccggt
     4081 cgatgaggaa ctctttggac agggcgcggg cgggatgttc caggaactgc accagcagat
     4141 cgtactcggt gctggtcaga tcgatctcgc ggtgcccgcg ctgcgcccgc cgggtgcccg
     4201 tatccaggct gagatcggca aagcgcagca ccggcgggcg ctcgctggcg cgccggcgca
     4261 gcagagcgtt cacgcgcgcc aggagcacct cgaaggtaaa gggcttgacc acataatcgt
     4321 cggcgccccc cgccaagccg gccacctggt ccgccggggc gtccttgccg gtcaacagca
     4381 ggatcggcag ttgcgggtcg gcggcgcgca gccgttgcag gacctccatg ccgctgaggc
     4441 ccggcatcat aatgtctaaa atcaccagat ccgggtagcg ttcccgcgcc agcgccagcc
     4501 cggcggcgcc ggcagcggcg gtgtcgacgc caaacccttc gtaggcgaga ccacgcttga
     4561 gcacactcgt caccgccggg tcgtcgtcga tcaccaggat ccgcgccatg ccccatgcct
     4621 cctttccttg tctacaccgc atcatagcac atcgcaggcc gatgtaggct ttcgatcagg
     4681 ttccgcttac acaccaccgg cgctgcggca ccggccgccg ggcatgcgtc cggcgccggt
     4741 gcctcggtgt cctcagtact cagacgatca gcagggtgaa gatgccaacg gtgatcgctg
     4801 ccgcgctggt ggcgagcttg agcacggccc ggcggtgcat ctgccagcgg gccaaccggc
     4861 ggtagaccag cggactgctg gagaacccca gaatgatgac gagcggcgcg ataaagacca
     4921 ggttgtagat caccaagtag gccaggccct gggcgaatgt cgcttgtgtc gagagcaacg
     4981 ccaggacccc aaggtagacc gaaccggcgc acggcacggt gcagagcccg acgaggaacc
     5041 cggcgaggag caaggccggg aagctgagct tcatcaccat ccccttcagc ttcacgtggt
     5101 cgacgtgcgc cgtcatacgg gtgccccact cgggcagcag cacctcttgc agggccagca
     5161 ggcccagccc cagcgcgaaa aaggccgcca ggcggctggc gaagtgggtg ctgctgacga
     5221 gcttcagtcc ctccaggctg ctcagcagcc ccaggccgag cgccaggtaa gtaatgaaaa
     5281 tcccgaccac atagactccg ccgtgcgtga ggagccagcc gcgggcggca cgttcgcccg
     5341 cgcgatccgg ccgccgttgg atcatcgcca gcatcgtggc gatgaagacc atcagcaagg
     5401 cgaatgcaca gggattaaga ctatcgacca ggccggcgcc catgaccacc ggcagcgtaa
     5461 ggggttggcc ttccatgctg aggagcctcc gtttagtaga aggtaagggt gccctgcata
     5521 ttggggctct tcttgccgcc gcagcacaca tcgcaatagt aggtataggt gcccggctga
     5581 tcggattgca gggtaatgac gcgcgactgc ttggcgtcga gtttcacgtc gatacccagg
     5641 gcgtcgaccg cgaactggtg cagggcaccg ctggcgtggt acgggctttc gttgttcacc
     5701 aggcgcacgg tgaccggctt gcccgccgga atacgcaggg tgcccg
//
LOCUS       DASUYC010001111         2469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_2039918, whole genome shotgun sequence.
ACCESSION   DASUYC010001111 DASUYC010000000
VERSION     DASUYC010001111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2039918"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(286..1200)
                     /locus_tag="VF276_18135"
     CDS             complement(286..1200)
                     /locus_tag="VF276_18135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608825.1"
                     /translation="MDTQRLHRIAYLFPEAPSAARAFFDYLAQFLGELPGTTAGASGI
                     GFAVTPPRGLPALSMALAAVAFPQVLFNTEAPIDLPLGAFALRSSAVAGDAETPPAPD
                     SRPAPHRADAVGPYVEVGSPDAASAQLALTELARRLAGRLVRLDHTGVNIPTTRVDRA
                     GWDALLHRLGGIANVYRYPTGEDWPFILPATDAEFADGITDFAAVREPKFELVYDSYA
                     REPVIQISVETDLLRSDLEALLPAPYGVAFPDLGDIFRTVFVHPPWPGLQIRCDLYSR
                     SAAPVDPWTSGEWLVTQGGRIGNGHPAG"
     gene            complement(1291..>2469)
                     /locus_tag="VF276_18140"
     CDS             complement(1291..>2469)
                     /locus_tag="VF276_18140"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX6608826.1"
                     /translation="APAPAPAPAPAAVAVAPAPAQAPATGGNGAGGGSKRISPAVARL
                     LEEHALDINQIQGTGLEGRVTKLDIERYLEQRAAGTLPAPAPAQPAAAAPAPAVAQAP
                     AAPAATAPVPGAGMAEAGMRSPYEPALPYTAPPPAAPAPPAPAAAAPAPPVAPVFQGA
                     GDQQVPLTQMRKAIAEHMVRSVHTSPHAWTYVEIDMNNIVKYRNRIKEQYQQREGLAP
                     SMIAIVIPSLVQALRQHPYVNSVWTDQGIVLKRDINVGIPIDLGGQGLIVPVIHQADT
                     LSFVGLTRTVNDLANRARSNKLRIDDLQGSTVTLNNSGTFGSVMSFPILNQPNVINIT
                     MEAIIKRPVVIDDAIAIRPMMNMCASFDHRVLDGATVGRFLQTMKKLLEGFTGEGFGY
                     "
BASE COUNT          381 a          791 c          933 g          364 t
ORIGIN      
        1 cctacgcggc ggcgggccga gccaggccgc caaacaaaaa ccggtccctg ttcaaggccg
       61 gtttcactat tggcgcccgc ccgctgggaa tcggcgggcg gctgccccag gctcaagtga
      121 ccacgtacaa gccggggaac acgctgcatt gaacgctctg ctattgccgg cagagtcgct
      181 gcttagcggc tcaggcgcaa ggcagactcc accgagttta ggatagcgcg ggcacgtgaa
      241 aaggccggta tagctgggtg gcagtttggt gagcgagccg gcgcgtcatc cggccggatg
      301 cccattcccg atccgcccgc cctgcgtcac cagccactcg ccgctggtcc aggggtccac
      361 gggcgcggcg ctgcgggagt agaggtcgca gcggatctgc aagcctggcc agggagggtg
      421 gacgaacacc gtgcggaaga tgtcgcccag gtcggggaag gcgacgccat agggggcagg
      481 cagcagggcc tccaggtcgg agcggagcag atcggtctcc acgctgatct ggatcaccgg
      541 ctcgcgggca taggagtcgt agaccaactc gaacttcggt tcgcggacgg cggcgaagtc
      601 ggtgatgccg tcggcgaact cggcgtcggt ggccgggagg atgaacggcc agtcctcgcc
      661 ggtgggatag cggtagacgt tggcgatccc gcccagccga tgcagcaagg catcccagcc
      721 ggcgcggtcc acccgcgtgg tcgggatatt caccccggta tggtcgagcc ggacgagacg
      781 cccggccagg cggcgggcca gttcggtcag cgccagttgc gcggacgccg cgtcgggcga
      841 tcctacttcg acatacggcc ccacggcgtc cgcgcggtgc ggcgccgggc ggctgtcggg
      901 cgcgggcggc gtctccgcat ccccggccac cgctgagcta cgcagcgcga aggcgcccag
      961 cggcagatcg atgggtgcct cggtgttgaa cagcacctgg gggaaggcca cggcggccag
     1021 ggccatcgac agcgccggga ggccgcgcgg cggggtcacg gcgaagccga tcccgctcgc
     1081 gccggcggtc gtgcccggta gctcgcccag gaactgcgcc agatagtcga aaaaggcgcg
     1141 cgccgcggaa ggagcctccg ggaacaggta ggcgatccgg tgcaatcgct gcgtatccat
     1201 gccacacctc cgccacaaaa agcggcgggg cgggtgaata cccgccccgc ccgtcttatt
     1261 cgtgtcgcgc gatggggcgc cgggcctggc ttagtagccg aagccctcgc cggtgaagcc
     1321 ttcgagcagc ttcttcatcg tctgcaggaa gcggcccacc gtggcgccgt cgagcacgcg
     1381 atggtcaaag ctcgcgcaca tgttcatcat cgggcggatg gcgatggcat cgtcgatgac
     1441 gaccgggcgc ttgatgatcg cctccatggt gatgttgatc acgttgggct ggttcaggat
     1501 cgggaacgac atgaccgagc cgaaggtgcc cgagttgttc agcgtcaccg tggagccttg
     1561 cagatcgtcg atgcgcagct tgttcgaccg ggcgcggttc gccaaatcgt tcacggtacg
     1621 cgtcaggccg acgaaggaca gggtgtcggc ctggtggatc accggcacga tcagaccctg
     1681 cccgcccagg tcgatcggga tcccgacgtt gatatcgcgc ttgagcacga taccctggtc
     1741 ggtccagacc gagttgacgt aggggtgctg gcgcagcgcc tggaccagcg acgggatcac
     1801 gatggcgatc atgctggggg ccaggccctc gcgctgctga tactgctcct tgatccggtt
     1861 gcggtacttg acgatgttgt tcatatcgat ttcaacatat gtccaggcgt gcggcgaggt
     1921 atgcaccgaa cgcaccatgt gctcggcgat ggccttgcgc atctgcgtca gcggcacctg
     1981 ctgatcgccg gccccctgga agaccggggc gacgggcggc gcgggcgcgg cggccgccgg
     2041 ggccggtggc gcgggtgcgg cgggcggcgg cgcggtgtag ggcagcgccg gctcgtaggg
     2101 gctgcgcatc ccggcctcgg ccatccccgc accgggcacg ggggccgtag cagcaggcgc
     2161 agcaggggcc tgggcaacag ccggggcagg cgcggcagcg gcgggctggg ccggggcggg
     2221 cgcgggcagc gtgccggcgg cgcgctgctc caggtagcgc tcgatgtcga gcttggttac
     2281 gcggccttcg aggccggtgc cctggatctg gttgatgtcc agggcgtgct cttcgagcag
     2341 gcgggccacg gcggggctga tgcgcttcga gccgccaccg gcaccgttgc cacccgtggc
     2401 cggtgcctgg gcaggcgcgg gcgcgacggc caccgcagcg ggcgccgggg ccggagcggg
     2461 cgcgggagc
//
LOCUS       DASUYC010001112         3203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1117587, whole genome shotgun sequence.
ACCESSION   DASUYC010001112 DASUYC010000000
VERSION     DASUYC010001112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117587"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="VF276_18145"
     CDS             complement(<1..1088)
                     /locus_tag="VF276_18145"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX6608827.1"
                     /translation="MDETLTDPIAVQRVELQQLEQDLSTLTRLAGDMLDLLRRSTNPG
                     DIGNAVAGLAPALQEQVHTAQQHRAAAALGLDAIARLAESRRQEAHHLRTLYTIVQAV
                     NSTLDLRALLNLVMDKVIEVTKAERGYVVLRDEQSGALTVAAARGMDHGTIDSDAFLI
                     SRGIVTQVATSAQPVLTTNAQADPRFSAMESVMHYNLRAILCAPLVIRGQVLGVVYVD
                     NRLRTGLFTPRDLDLLVNLANQAAVAIDNARLYGYMANVLASIASGVVTTDEYGIIRT
                     YNRAAAGIFAIQPEQAIGQSYRDVFGLLANSPVPALFTQVQESGEPVLGHEARAVVPG
                     RGEVVLLLNVARVVAETGEKLGVVVVIE"
     gene            complement(1128..>3203)
                     /locus_tag="VF276_18150"
     CDS             complement(1128..>3203)
                     /locus_tag="VF276_18150"
                     /inference="COORDINATES: protein motif:HMM:NF024764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6608828.1"
                     /translation="DAPESAGGTQPVAVASPDTPLTERVAPPLPVEPLGIAPPLPAEP
                     VKVEPPASAEAILPAPADLLPAAPPPGTKMAAAARLGMGRMSCSMAVSVVAVLLLLIG
                     GVSYLSLGRLPLGQPPGGNSAASSPPTSYAVVAMPPDSEVAGSPLPTTLAAAAPPGGG
                     SGDQHATATAAANGAQATAAAMQAATAVAADPSRALQTGDQALAAKQYAKALAVYDAV
                     LTGDPQNADALRGQGLALLGLGRYQDAINALTQALGAHPDDPALLLARAQASAGLQSW
                     SDVAQDTERILSQDKAAVPALLLHAQAAAMNGGDARAVADLDTAVAAAPQDPAVYKAR
                     ADYYVNNRRLAAAVADYQKALSFAPDDAGIWIALGRAYITYDEAIQPQPDQALAAFGH
                     ALQVDPRNAQAYYWRARVYQDYKSDRADALADINQAITIGPPTADMYYLRATLEGQLA
                     NDKAQLLDLQRAVTLEPKNTQVYIWLAEYYAAHQQYDRTAETLTRLIAVDNNYYYYGA
                     RSEIYLLLGDYARARADAQQAVTLRADAAYGYAQLAHVFTVQRDFTSALDQINQALDH
                     AAADEKADLLAYRARINLGRSQPDQAAADLTAAAAINADNANVLLEQAELAVAKKQPA
                     QALEYLSRWDGKDDRYGWGYVVRAGIEAGQNQPVKARADLQQARKLALFPDERQAAAE
                     IAARLGAAP"
BASE COUNT          456 a         1047 c         1162 g          538 t
ORIGIN      
        1 tcctcgataa cgacgacgac gcccagcttt tcgcccgttt cggccacgac gcgggccacg
       61 ttgagcagca gcaccacctc gccgcgcccc ggcaccacgg cgcgggcctc gtgccccagc
      121 accggctcgc ccgactcctg cacctgggta aacagggccg gcaccggcga gttggccagc
      181 aggccgaaca cgtcgcggta cgactggccg atggcctgtt cgggctggat ggcgaaaatg
      241 cccgcggcgg cgcggttgta ggtgcggatg atgccgtact cgtcggtcgt gaccacgccg
      301 ctggcgatac tggcgagcac gtttgccatg tagccgtaca gccgtgcgtt gtcgatcgcg
      361 acggcggcct ggttggcgag gttgaccagc aggtcgagat cgcgcggggt gaacagcccg
      421 gtgcgcaggc ggttgtccac gtaaactacg cccagcactt ggccgcggat caccagcggc
      481 gcgcacaaga tcgcgcgcag gttgtagtgc atgacgcttt ccatggccga gaagcgcggg
      541 tcggcctggg cgttcgtggt cagcaccggc tgggcgctgg tcgccacctg ggtgacgatg
      601 ccccggctga tcaggaaggc gtcgctgtcg atggtgccgt ggtccatgcc gcgggcggcg
      661 gccacggtca gcgcaccgct ctgttcatcg cgcagcacca catagccgcg ctcggccttc
      721 gtcacctcga tgaccttgtc catcaccagg ttgagcaggg cacgcagatc gagcgtcgag
      781 ttgacggcct gcacaatcgt gtagagcgtg cgcaggtgat gagcttcctg gcggcgcgac
      841 tcggccagcc gggcgatggc gtcgaggccc agggcggcgg cggcgcggtg ctgctgggcg
      901 gtatgcactt gctcctgcag ggcgggggcc agcccggcca ccgcgttgcc gatatcgccc
      961 ggattggtcg agcggcgcag cagatcgagc atatcgcccg ccaggcgggt gagtgtgctc
     1021 agatcttgct caagctgttg gagctcgacc cgctgcacgg caatcggatc ggttaacgtt
     1081 tcatccatcg cagttcctct gtttcgcggg gcaccaacgc ggcgcgctca cggtgcggcg
     1141 ccgagccggg ccgcgatctc ggcggcggcc tgccgctcat ccgggaagag cgccagcttg
     1201 cgcgcctgtt gcagatcggc gcgggccttc accggctgat tctggccggc ctcgattccg
     1261 gcgcggacga cgtagcccca gccgtagcga tcatccttgc cgtcccaccg gctcagatac
     1321 tccaacgcct gcgccggttg ctttttggcg accgccaatt ccgcctgctc cagcagcacg
     1381 ttcgcgttgt cggcgttgat ggccgcggcg gcggtcaggt cggcggcggc ctgatccggt
     1441 tgcgaccggc ccaggttgat ccgggcgcgg taggccagca aatcagcctt ctcgtcggct
     1501 gccgcatggt ccagcgcttg gttgatctgg tcgagcgcgg acgtaaaatc gcgctggacc
     1561 gtgaagacgt gggcaagctg agcatagccg taggccgcgt cggcacgcag agtgacggcc
     1621 tgctgggcgt cggcgcgcgc cctggcgtag tcgccgagca gcagatagat ttcgctgcgt
     1681 gccccataat aataatagtt attgtccact gcgatcaagc gggtcagcgt ttcggcggtc
     1741 cgatcgtact gctggtgggc ggcatagtat tcggctagcc agatatagac ctgggtgttc
     1801 ttcggttcga gcgtcacggc ccgctggaga tccagcaact gggctttgtc gttcgccagt
     1861 tggccctcca gcgtggcacg caggtaatac atatcggcgg tgggcggccc gatggtgatg
     1921 gcctggttga tgtcggcgag ggcatcggcg cggtcactct tgtagtcctg ataaacgcgt
     1981 gcccgccagt agtaggcctg ggcgttgcgc gggtccacct gcagcgcgtg accgaaggcg
     2041 gccagcgcct ggtcgggctg gggctggatg gcctcgtcgt aggtgatata ggcccggccc
     2101 agcgcgatcc agatcccggc atcatcgggt gcgaagctga gcgccttctg gtaatcggcg
     2161 actgccgcgg cgaggcggcg gttattgaca taataatcag cccgtgcctt gtagacagcc
     2221 ggatcctgcg gcgccgccgc gaccgccgtg tcgaggtcgg ccacggcgcg cgcatcgccg
     2281 ccgttcatgg ctgcggcctg ggcatgcagc agcagggccg ggaccgccgc tttgtcctgg
     2341 ctaaggatgc gttcggtgtc ctgggccacg tcggaccacg actggaggcc cgcgctcgcc
     2401 tgggcacgcg ccaggagcag cgccgggtcg tccgggtggg cgccgagggc ctgggtcagg
     2461 gcgttgatcg cgtcctggta gcggcccagg ccgagcaagg ccaacccctg gccgcgcaag
     2521 gcgtccgcgt tctgcggatc gccggtcagc acggcgtcat agacggcaag ggccttggcg
     2581 tattgtttcg ccgccagggc ctgatcgccg gtctgcaggg cgcggctcgg atcggcggcg
     2641 acggcagtgg ccgcctgcat ggccgccgca gtggcctgcg ccccattcgc ggcggcggtg
     2701 gccgtggcat gttgatcccc gctccctccg cctggcggcg ccgcggcggc gagagtcgtc
     2761 ggaagcggac tgccggcgac ttcgctgtcc ggcggcatgg cgacgacggc atagctggtg
     2821 ggcgggctgc tcgccgcact gtttccacct ggcggctgcc ctaacggcag ccggcccagg
     2881 ctcaggtagc tcacgccgcc gatcagcagc agcaggaccg cgacaacgga aacggccatc
     2941 gaacacgaca tgcgccccat gcccagccgg gcggcagcgg ccatcttggt gccgggcggc
     3001 ggggcagcgg gcagcagatc cgccggggcc ggtaaaattg cctcggcgga ggcgggcggt
     3061 tcgaccttta ccggctcagc gggtagaggc ggcgcgatcc ccaagggctc gacgggtagg
     3121 ggcggcgcga cccgttcggt gagcggcgta tccggcgacg ccacggcgac cggctgggtg
     3181 ccgccggcgc tctccggcgc gtc
//
LOCUS       DASUYC010001113         4469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1809108, whole genome shotgun sequence.
ACCESSION   DASUYC010001113 DASUYC010000000
VERSION     DASUYC010001113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4469
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1809108"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1609
                     /locus_tag="VF276_18155"
     CDS             <1..1609
                     /locus_tag="VF276_18155"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX6608829.1"
                     /translation="NIMREEINAIGGQELTMPVVHPADIWKETGRWYQIGSELGRFFD
                     KSNRDMVLAMTHEEVVTDLVRKEINSYRQLPQLIYHIQTKWRDDPRPRAGLIRVREFT
                     MVDSYSLDAEWEGLDRQYREHYQAYFNIFNRTALPVLAVKSDVGMMGGSMAHEFMYLT
                     PIGEDTLLLCDNCGYAANRQIARFAKPPAAPEAPLPLEKIATPATTTIESLAELLGVP
                     RAKTAKAVFLVATVTEGDRDVDRFVFAVVRGDMDVNETKLANAVKAKELRPARTEEIQ
                     VVGAVPGYASPIGLRDIVVVVDDIIPDSPNLVAGANEEGYHLLNTNYGRDYTATVVAD
                     IAVARAGDACPVCGAPLRTSGGVETGNIFKLGTRYSAAMGATFLDAEGKSRPVIMGSY
                     GIGLGRLLACVVEEHHDDKGIIWPISIAPYQVHLVALANERGAAAATADQLYADLTAA
                     GVEVLYDDRAESPGVKFMDADLIGIPLRLTVGERGLKRGGVELKRRTSAESTLVPVAD
                     VVEAVRAEIARLEEAVAARIMPVPFNG"
     gene            1616..>4469
                     /locus_tag="VF276_18160"
     CDS             1616..>4469
                     /locus_tag="VF276_18160"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX6608830.1"
                     /translation="MDSTAAPVPIVLHGAWVPADGGASGALLLWGERLVRPAPTANAS
                     APTGDSPAPHPFAAGEADLRAALHTVGAGFKPALGALPAAWFPAIPLWLPQGAGGGPQ
                     PSPALDPAPGAPGPLRPWTVGALALDALGALALLLRLPATAPLPVGSGLHFWRAAARL
                     AAAMLAGGRYLPGFRSDDEGAWASWDPSWSDPGDNAARARLVAAMPLVAAAATHPADP
                     RRLLDSFIRATIGAAARAWAPAEALPAPPRSGAPTAQHAAYEWMLALLGADPLVAAEP
                     AAWHAFTRQYRTWHDTQQPAEGTPSFRVCFRLEPPAESADPWRLHFLLQATDDPSLLV
                     PAAQVWQMTGGRAAALPLRGRDFARPPERFRAGLAGAGRLFAPIADALRHPAPINSPL
                     TPVQAYSFLREAAPLLQAAGYGVLVPPWWGTRAARPVVRLQVREAAPVASPPPAGGPG
                     LLGADALVDFDWQVAAGDVVLDRAEFERLVALKSPLVNVKGQWVEFRPGEVEAALRFW
                     TQQRAHGPAPLAVVARLLLGGDGAADAGLPLDEEAGALSPAVTEALVVLREGLHAPDL
                     PPPLELQATLRPYQVRGFSWLAGLVRLGLGACLADDMGLGKTIQTIALLLHRRAGMPA
                     LLVCPTSVVGNWRREIDRFAPSLRVLVHQGPQRAAGPAFAATARAHDLVITSFSLLAR
                     DEATLQTVTWAGLILDEAQNIKNPDAAQTRAARRLRAGYRVALTGTPVENRLQELWSI
                     IDFLNPGFLGDRESFRAQYALPIEREGDPAAMDALRRRVNPLILRRLKTDRAIIQDLP
                     DKLELKVYCTLTREQVTLYEAVVRDGMAQLAQLAGRGAFARRGVVLSLLTRLKQIVDH
                     PALFLSDGSPLPDRSGKLARLAEMLDEALAEGDKALIFTQFAEMGAMLQQYLAATFAR
                     PVLYLHGGTPRPQRDAMVAEFQAPGGPALFVLSIKAG"
BASE COUNT          621 a         1589 c         1613 g          646 t
ORIGIN      
        1 aaacatcatg cgcgaggaga tcaacgccat cggcggccag gagctgacga tgccggtggt
       61 ccacccggcc gacatttgga aggaaaccgg gcgctggtac cagatcgggt ccgaactggg
      121 gcgtttcttt gacaagtcga accgcgacat ggtgctggcg atgacccacg aagaggtcgt
      181 gaccgacctg gtgcgcaagg agatcaactc ctatcgccag ttgccacagt tgatctacca
      241 tatccagacc aagtggcgcg acgacccgcg cccgcgcgcc gggctgatcc gcgtacgcga
      301 gttcaccatg gttgacagct acagcctcga cgccgagtgg gaggggctgg accggcaata
      361 ccgcgaacac taccaggcct atttcaacat cttcaaccgc accgcgctgc cggtgctggc
      421 ggtgaagtcc gacgtgggca tgatgggcgg cagcatggcc cacgagttca tgtacctgac
      481 gcccatcggc gaggacacgc tgctgctctg cgacaactgc ggctacgcgg ccaaccggca
      541 gatcgcccgc ttcgccaagc cgcccgccgc gcccgaagcg ccgctgccgc tggagaagat
      601 cgccacgccc gccaccacga ccatcgagag cctggccgag ttgctcggcg tgcccagagc
      661 aaagacggcc aaggcggtct tcctggtggc gaccgtgacc gagggcgacc gggacgtgga
      721 ccgcttcgtc ttcgcggtgg tgcgtggcga catggacgtg aacgagacca agctggccaa
      781 cgccgtgaag gccaaggaac tgcgcccggc gcgcaccgag gaaatccagg tcgtgggcgc
      841 ggtgcccggc tacgcctcgc ccatcggcct gcgcgacatc gtcgtggtcg tcgacgatat
      901 catcccggac tcgccgaacc tggtggccgg ggccaacgag gaaggctacc acctgctcaa
      961 cacgaactat ggccgcgact acacggcgac ggtggtggcc gacatcgccg tggcccgcgc
     1021 gggcgacgcc tgcccggtgt gcggcgcccc gctgcgcacg tcgggcggcg tggagacggg
     1081 caacatcttc aagctgggca cgcgctacag cgcagccatg ggcgccacct tcctggacgc
     1141 tgagggcaag agccgcccgg tgatcatggg gtcctacggc attgggctgg ggcggctgct
     1201 ggcctgcgtg gtcgaggagc accacgacga caaaggcatc atctggccga tctccatcgc
     1261 cccgtaccag gtccatctgg tggcgctggc gaacgaacgg ggcgccgccg cggccaccgc
     1321 cgaccagctt tacgccgatc tgacggcggc gggcgtcgaa gtgctctatg acgaccgcgc
     1381 ggaaagcccc ggcgtgaagt tcatggacgc cgacctgatc ggcatccccc tgcggctgac
     1441 ggtgggcgag cgggggctga agcgcggcgg cgtcgagttg aagcgccgca ccagcgcgga
     1501 gagcacgctg gtgccggtgg ccgacgtggt agaggcggtg cgggccgaga tcgcgcggct
     1561 ggaggaagca gtggccgcca ggatcatgcc agtgcccttc aacggctagg cgcgcatgga
     1621 ctcgacggca gccccggtgc cgatcgtgtt gcacggcgcc tgggtgccgg cggacggcgg
     1681 ggcgagcggt gcgctgctgc tctggggcga acggctggtc cgccccgcgc ccacagccaa
     1741 tgcatctgcc cctaccggcg atagccccgc gccgcacccc ttcgccgccg gagaggccga
     1801 tctccgcgcc gcgttgcata ctgtaggggc cggtttcaaa ccggccctcg gtgccttgcc
     1861 cgccgcctgg tttccggcga tcccgctctg gctgccgcaa ggcgcgggcg gcgggccgca
     1921 accatccccc gcgctcgacc cggcgcccgg tgcgcccggc ccgctgcgcc cctggaccgt
     1981 gggcgcgctg gcgctggatg cattgggcgc gctggcgctg ctcttgcgct tgcccgctac
     2041 ggcacccctc cccgtcgggt caggcctgca cttctggcgg gcggcggcgc ggctggcggc
     2101 ggccatgctg gccgggggcc gctacctgcc cggcttccgt agcgatgatg agggcgcctg
     2161 ggctagctgg gatccgagtt ggagcgatcc cggcgacaac gcggcgcgtg cccggctcgt
     2221 ggccgccatg cccctcgtcg cggcagccgc cacccatccc gccgacccgc gccgcctgct
     2281 cgatagcttc atccgtgcaa ccatcggcgc ggcggcgcgc gcctgggcgc cggcggaggc
     2341 gctgccggct ccgccgcgca gcggagcgcc cacggcgcag catgctgcct atgagtggat
     2401 gctggccctg ctgggcgccg acccgctggt ggcggcggag ccggcggcct ggcacgcctt
     2461 cacccgccag taccgcacct ggcacgatac gcagcagcct gcggagggca cgccctcgtt
     2521 ccgcgtctgc ttccgcctgg agccacccgc ggagtccgcc gatccgtggc gactgcactt
     2581 cctcttgcag gccaccgacg accccagcct gctggtgccc gccgcgcagg tctggcagat
     2641 gaccggcgga cgggcggcgg cgctgccgct gcgcgggcgc gacttcgccc ggcccccgga
     2701 acgcttccgc gcggggctgg cgggggcggg ccgcctattc gcgcccatcg ccgacgcgct
     2761 gcgccacccc gcgccgatca acagcccgct gaccccggtt caggcgtaca gctttttgcg
     2821 cgaagccgcg ccgctgttac aggcggcggg ctacggggtg ctggtgccgc cgtggtgggg
     2881 cacacgcgcc gccaggccgg tggtgcgttt gcaggtgcgc gaggcggcgc ccgtggcgag
     2941 tccgcccccg gcaggcgggc cggggctgct aggcgccgat gcgctggtcg atttcgactg
     3001 gcaggtggcc gccggggatg tcgtgctgga ccgggccgag tttgagcggc tggtggcgct
     3061 caagtcgccg ctcgtcaacg tgaagggcca gtgggtcgag ttccggcccg gcgaggtcga
     3121 ggcggcgctg cgcttctgga cgcagcagcg cgcgcatggc cccgcgcccc tggcggtggt
     3181 cgcccgtttg ctgctgggcg gcgacggcgc ggcggatgcc gggctgccgc tggacgagga
     3241 ggctggggcg ctgtcgcccg ccgtgaccga ggcgctggtc gtgctacgcg agggactgca
     3301 cgcgccggat ttgcccccgc ccctggaatt gcaggcgacg ttgcggccct accaggtgcg
     3361 cggcttctcc tggttggccg ggttggtgcg cctgggcctg ggcgcctgcc tggccgacga
     3421 catgggcctg ggcaagacga tccagaccat cgccctgctg ctccaccggc gggccgggat
     3481 gcccgcgctg ctggtctgcc ccacatcggt ggtgggcaac tggcggcgcg agattgatcg
     3541 gtttgccccg tcgttgcgcg tgctggtcca ccaggggccg cagcgggcgg cggggccggc
     3601 gttcgcggcg acggcacgcg cccacgatct cgtcatcacc tcgttcagcc tgctggcccg
     3661 cgacgaggcc actctccaga ccgttacgtg ggccgggctg atcctcgacg aggcgcagaa
     3721 catcaagaac ccggacgccg cccagactcg tgccgcacgc cgcctgcggg cgggctaccg
     3781 ggtggccctg accggcaccc cggtcgagaa ccgtctgcaa gagttgtggt cgatcataga
     3841 cttcctcaac cccggcttcc tgggcgaccg cgagtcgttc cgcgcgcaat acgccctgcc
     3901 catcgagcgc gagggcgacc cggcggccat ggacgccctg cgccgccgcg tcaacccgct
     3961 gatcttgcgc cgcctcaaaa ccgaccgcgc gatcatccag gatttacccg acaagctgga
     4021 gctgaaggtc tactgcacac tgacccgcga gcaggtgacg ctctacgagg cggtggtgcg
     4081 cgacgggatg gcccaactgg cgcaactggc gggccggggg gcctttgcgc gccggggcgt
     4141 ggtgttgagc ctgctgacgc ggctcaagca gatcgtggat caccccgcgc tgttcctgag
     4201 cgacggcagc ccgctgcccg accggtcggg caaactggcc cggctggccg agatgctgga
     4261 cgaggcgctg gccgagggcg acaaggcgct gatcttcacc cagttcgccg agatgggcgc
     4321 catgttgcag caatacctgg ccgcgacgtt cgcgcggccc gtgctctacc tgcacggcgg
     4381 cacgccgcgc ccgcaacgcg acgccatggt cgctgagttc caggcgcccg gcggcccggc
     4441 cctgttcgtc ctctcgatca aggcgggcg
//
LOCUS       DASUYC010001114         4302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_448547,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001114 DASUYC010000000
VERSION     DASUYC010001114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448547"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /gene="sucC"
                     /locus_tag="VF276_18165"
     CDS             complement(<1..947)
                     /gene="sucC"
                     /locus_tag="VF276_18165"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEX6608831.1"
                     /translation="MKIHEYQAKEILARHGIPVNSGELATTPEEAEAIARKIGKMVVV
                     KAQVLVGGRGKAGGVKLAKTPEEAREKAQQILGMDIKGITVQKVLIADAADIAQEYYL
                     GAILDRGSKQIVVMASAAGGVEIEEVARETPELIHRVGVDPLIGFQDFQARDLAYAME
                     IPAPLIRDFVKICRGLVDALIASDASLAEINPLIRTPDGKLLAIDAKMDLDDFGLARH
                     PDLEALRDPNEDIPVEKAAREAGLSYVKLDGDIGCMVNGSGLAMGTMDAIKLHGGEPA
                     NFLDVSGGANEQQVTTAMRLILSDPKVNAVLINIFGGITR"
     gene            complement(1287..3797)
                     /locus_tag="VF276_18170"
     CDS             complement(1287..3797)
                     /locus_tag="VF276_18170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX6608832.1"
                     /translation="MSAGLSRLKLLPPRGAVDPAAPAPPPAAKPRPVAPTAPPKSRER
                     RVGRPQMPKLPRPRVRLPRVPRPRLPKPATWTRYTLGLAGLLILGLLVRLPLLWAAPL
                     DTPSAPQATMAAFVRRLATTGGDLKAALPWIGPLPIDLTGGLPLYAWVAALLSGFLGP
                     QAWIGRALALAGVLAATGLLFVVVRRLAGGRAALYAALFLTVTPPGLYYGRAYLPETW
                     GWAAACAALAAALRWRDCVLAGRPDERRWFRIAAATGGLALLLSPANLALLPPLLYLS
                     RSLGPPAATDSLALDMARARRTGNEVYLYAALLVGPLLLWWALTHIGGAVSEVDPAFG
                     GGGIGAALAELRRGEFYGLLAGRLVNGTLTVAGFLLLLAGLGRPARAPWPWALHLWAG
                     TGLVVVLGNATRLAADDSTLVAWLPALAAVAGLGANWVAALPAAIVAALRGHEEEAEI
                     LEADLDDGAPADEAAPGTWLGARRPPRGAVRRAGWSQGTGARPEQSWPRTRTALLRLG
                     NAVAVILLLAVFAGGFGALTARYEVSAAARLYEVVGSRAAATGPGHAGGRMVVAGPGA
                     PEMFYTAGLTGWAVPQDRFSRAALDQMKQAGAVLLISADQTWLGRQPDYPGLVASFQV
                     ALLAQEYIIFDLHNPPPTRDSLYFLETGHTLRGSFRTFWQSNGGVARFGFPLTEELQE
                     VSPEDGKERTVQYFERAVMELHPDQAGTPYDVQLAPLGRRLLDAQIKRADSLGTQVVG
                     VDPVPEAPNTPTFHFFKETGHSVKGEFLKTWQAGGVRVYGYPISEEMREISPADGKVH
                     IVQYFERARFEWHQEFAGTPNQVQLGLIGREWLDAQKP"
     gene            complement(4082..>4302)
                     /locus_tag="VF276_18175"
     CDS             complement(4082..>4302)
                     /locus_tag="VF276_18175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEX6608833.1"
                     /translation="GDTDTNAAIAGALLGAIYGAGTVPDQWRAVLLDCRPAQGAPGVH
                     RPRPEMYWPVDALDLAGQLLAAGRRAAV"
BASE COUNT          624 a         1550 c         1496 g          632 t
ORIGIN      
        1 cgggtgatgc cgccgaagat gttgatcagc acggcgttca ccttggggtc gctcaggatc
       61 aggcgcatcg ccgtcgtcac ctgctgctcg ttggcgccgc cgctcacgtc gaggaagttg
      121 gccggctcgc cgccgtgcag cttgatggcg tccatggtgc ccatcgccag cccggagccg
      181 ttgaccatgc agccgatgtc gccgtcgagc ttcacatagc tcagcccggc ctcgcgggcc
      241 gccttttcga cggggatgtc ttcgttgggg tcgcgcagcg cctccaggtc ggggtgccgg
      301 gccaggccga agtcgtcgag gtccatcttg gcgtcgatgg cgagcagctt gccgtcaggc
      361 gtgcggatca gcgggttgat ctcggccagg ctggcgtcgc tggcaatcag cgcgtcgacc
      421 agcccgcggc agatcttgac aaaatcgcgg atcagcggcg cggggatctc catggcatag
      481 gccaggtcgc gcgcctggaa gtcctggaag ccgatcagcg ggtccacgcc cacgcggtgg
      541 atcagttcgg gcgtctccct ggcgacttcc tcgatctcca cgccgcccgc cgcgctcgcc
      601 atgaccacga tctgcttgct gccgcggtcc aggatggcgc ccagataata ctcctgggca
      661 atatcggcgg catcggcgat cagcaccttc tgcacggtga tacccttgat atccatgccc
      721 aggatctgct gggctttctc gcgggcttcc tcgggggtct tcgccagctt caccccgccg
      781 gccttgccgc gcccgcccac cagcacctgc gccttcacga ccaccatctt gccgatcttg
      841 cgcgcaatcg cctccgcttc ctcgggcgtg gtcgccagct caccagaatt gaccgggatg
      901 ccgtgccggg ccaggatctc cttggcctgg tattcgtgta tcttcataga atctcctcgt
      961 tgagataaac taaactcaaa aagccgagcc ggtaacaacc gcctgcggcc gcgtgcggtg
     1021 cccctgccgg cggcagaaag ggccgccgga ttataacata tttcccccgc ttctgaacac
     1081 cgcgcgccgt gcagtctcgt ctccgtgcgt ccgtgtcact ctgagcgcag cgaagagtct
     1141 cgctccccgg cgtgtcactc tgagcgcagc gaagagtctc gctccccggc gtgtcactct
     1201 gagcgcagcg aagagtctcg tctccgtggc gcagagacga gatgcttcac tgcgttcagc
     1261 atgacaagtt cggttggagg cagcattcag ggcttctgcg cgtcgagcca ttcgcgcccg
     1321 atgaggccga gctgcacctg gttcggcgtc ccggcgaact cctggtgcca ctcgaagcgg
     1381 gcgcgctcga agtattgcac gatgtggact ttgccgtccg ccgggctgat ctcgcgcatc
     1441 tcctcgctga tcgggtagcc gtagacgcgg acgccgcccg cctgccaggt tttcaagaac
     1501 tcgcccttga cgctgtgccc ggtctccttg aagaagtgga aggtgggagt gttgggcgct
     1561 tcgggcaccg ggtccacgcc caccacctgg gtgccgagcg agtcggcacg tttgatctgc
     1621 gcgtcgagca gccggcggcc cagcggcgcc aattgcacgt cgtagggcgt gccggcctgg
     1681 tcggggtgca gttccatcac ggcccgctcg aaatactgca ctgtgcgctc tttgccgtct
     1741 tccgggctga cctcttgcag ttcctcggtc agcgggaagc cgaagcgggc cacgccgccg
     1801 ttgctctgcc agaaggtgcg gaacgagccg cgcaacgtat ggcccgtctc caggaagtag
     1861 aggctgtcgc gggtgggcgg cgggttgtgc aggtcgaaga tgatgtactc ctgcgccagc
     1921 agcgccacct ggaacgaggc gacgaggccg ggataatcgg gctggcgccc tagccaggtc
     1981 tggtcggcgc tgatcagcag caccgccccg gcctgtttca tctggtcgag cgcggcgcgg
     2041 ctgaagcggt cctgcggcac ggcccagccc gtcaggcccg cggtatagaa catttccggc
     2101 gcgcccggcc cggccaccac catgcgcccg ccggcatggc ccggcccggt ggccgcggcg
     2161 cggctgccca ccacttcata gagcctggcc gcggcgctga cctcgtagcg ggccgtgagc
     2221 gcaccgaagc ccccggcgaa aaccgccagc aagaggatca cggcgacggc gttgcccagg
     2281 cgcagcagcg ccgtgcgcgt gcgcggccag ctctgctcgg ggcgggcgcc cgtgccttgc
     2341 gaccagcccg cgcgccgcac ggctccgcgc ggcgggcgcc gcgcgcccag ccacgtccct
     2401 ggcgccgcct catcggcggg cgcgccgtcg tccaggtcgg cttccaggat ctcggcttcc
     2461 tcttcgtgcc cgcgcagcgc ggcgacgatg gccgcgggca gcgccgccac ccagttggcg
     2521 cccagcccgg ccacggcggc cagcgccggc agccaggcca cgagcgtcga gtcgtcggcg
     2581 gccaggcgcg tcgcgttgcc tagcacgacg accagcccgg tccccgccca caggtgcaac
     2641 gcccacggcc agggcgcccg cgccgggcgg cccaggccgg ccagcagcaa caggaagccg
     2701 gccaccgtca gcgtgccgtt gaccagccgc ccggccagca ggccgtagaa ctcgccgcgc
     2761 cgcaactcgg ccagcgccgc gccgatgccc ccgccgccga acgccgggtc cacctcgctc
     2821 accgccccgc cgatgtgcgt cagcgcccac cagagcagca gcggccctac cagcagcgcg
     2881 gcatacaggt acacctcgtt gccggtgcgc cgggcgcggg ccatgtccag ggccagcgag
     2941 tccgtggccg cgggcgggcc gagcgaccgg ctgagataaa gcagcggcgg cagcaaggcc
     3001 aggttggcgg ggctgagcag cagcgccagg ccgccggtgg ccgcggcgat gcggaaccag
     3061 cggcgctcgt ccgggcgccc ggccagcacg cagtcgcgcc agcgcaaggc ggcggccagc
     3121 gcggcacacg ccgccgccca gccccatgtc tcaggcaggt aggcgcggcc atagtagagg
     3181 ccgggcgggg tcacggtgag gaacagcgcc gcgtagagtg cggcccgccc gcccgccagc
     3241 cggcgcacca ccacgaacag caacccggtg gcggccagca ccccggccag ggccagcgcc
     3301 cgcccgatcc aggcttgcgg ccccaggaac ccgctgagca gcgcggccac ccaggcgtac
     3361 agcggcagcc cgcccgtcag gtcgatgggc agggggccga tccagggcag cgccgctttc
     3421 aggtcgccgc cggtggttgc cagccggcgc acgaaggccg ccatcgtcgc ctgcggcgcg
     3481 ctgggcgtgt ccagcggcgc ggcccagagc aggggcaggc gcaccagcag gccgaggatc
     3541 agcagtccag ccaggccgag ggtgtagcgg gtccaggtgg cgggcttagg cagccgcggg
     3601 cgcggcacgc gcggcaggcg cacccgcggg cggggcagtt tcggcatctg gggacgcccc
     3661 acccggcgct cccggctctt aggaggcgcg gtgggtgcga caggtcgcgg cttggcggcg
     3721 ggcggcgggg ccggggcggc ggggtcgacg gcgccacggg gcggcagcag cttcaggcgg
     3781 gatagcccgg cgctcatcgc ccggactcct cccccacgtg gtcgtggccg ggtcccaggg
     3841 gtgtatgcgg tttcatggcg gactccggcg gggccgtcac taccccggct ggcggaaatg
     3901 gtgatgcgat atgtgtatca taccagagcc gcccgttgtt ggcaaggccg ttaccggccc
     3961 atcacccggc ccgtacgcag gcacatttca cttgtcactc tgagcgcagc gaagagtctc
     4021 gtctcgtggg ggatcagacg agatgcttca ctgcgttcag catgacaagg ctggggtaaa
     4081 cctacacagc cgcccgacgg cccgccgcca gcaactgccc cgccaggtcg agcgcgtcca
     4141 ccggccagta catctccggg cgcgggcggt gcactcctgg cgccccctgc gcgggccggc
     4201 agtccaataa caccgcgcgc cactggtcgg gcaccgttcc ggcgccatag attgcgccga
     4261 gcagcgcccc ggcgatggcc gcgttggtgt cggtatcgcc ac
//
LOCUS       DASUYC010001115         3483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_2040367, whole genome shotgun sequence.
ACCESSION   DASUYC010001115 DASUYC010000000
VERSION     DASUYC010001115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2040367"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="VF276_18180"
     CDS             <1..638
                     /locus_tag="VF276_18180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189660.1"
                     /note="membrane component of a putative sugar ABC
                     transporter system; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HEX6608834.1"
                     /translation="HFFKVQAFIVTLMGLFLARGLAYIISLTSVTINDGFYKYLALTQ
                     IPMPFSRQAYVYIPSLVGPLLLLLAIYITFFTRFGRTIYAIGNNEQSARLMGLPVGQT
                     KILVYSFSGFCSALAGIVFSISLLAGYGQFATGMELDTIAAVVMGGTLLTGGVGNVVG
                     TLFGVLIEGTIISILQYNGTLSSWWTRIGVGVLTLIFIGIQSLFYIRRKHS"
     gene            830..982
                     /locus_tag="VF276_18185"
     CDS             830..982
                     /locus_tag="VF276_18185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX6608835.1"
                     /translation="MLRLHAAARHFGGAVKGFLAGPARGQIHVEQLPGYAPDLNPDEG
                     VWGQLK"
     gene            complement(1112..1942)
                     /locus_tag="VF276_18190"
     CDS             complement(1112..1942)
                     /locus_tag="VF276_18190"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6608836.1"
                     /translation="MSNQGQGYLLQGGAAELERLRLQARVWEPEAQALLDRIGVPPGA
                     TGVDLGCGAMGILGPLSTRVGADGRVVGVDVDDTQLAAARAYVADNGFTNVEIRKADA
                     YNTGLASEAFDLVHVRFLFAPVGRDEELLAEMLRLTKPGGLLVIQEPDATPWTCLPAS
                     AAWNQLKTTILAAFRLGGGDFNAGQRTYKMLRDAGLREVDLRAAVIALHDAHPYMRLP
                     IQFATSLRPRILAARLLTAADLDGAIAECEEIASRPDTSVLTFIVTQVWGRKAYRSLH
                     "
     gene            1981..2439
                     /locus_tag="VF276_18195"
     CDS             1981..2439
                     /locus_tag="VF276_18195"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX6608837.1"
                     /translation="MVVPEPVSTRAGALLTVAAAPGVPPPRICVPWRWVPGRFVRSGL
                     RPHHLRQVGRLTARLHDHAQGFQPPPGFSRPRIDDLPATRAAVLPLVAGIRPPRDVAL
                     VAAVLDRVAATLAGLDPGPDAYGLIHSDLHQWNYLFQHGQVHLLDFDDCG"
     gene            2506..2712
                     /locus_tag="VF276_18200"
     CDS             2506..2712
                     /locus_tag="VF276_18200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608838.1"
                     /translation="MCAALLAGYEATRPLPPAPETLLTTLTHLRHIQNLVWDIGLHGA
                     LYGRDHRQQIVASELDGLRAFLTG"
     gene            complement(2907..>3483)
                     /locus_tag="VF276_18205"
     CDS             complement(2907..>3483)
                     /locus_tag="VF276_18205"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6608839.1"
                     /translation="ATALEAAASLDPATTGRDRARALYGAGFLAQEQGDLPAASDLLE
                     QSLTLAREYGDLLTVARALRSLGDAVSGYGRGDLAAARTLYEQSLTQFRELGDQRNIA
                     MGFVALGNLALAGGDYTGARALLEDALTQFQKLGDVRLVAHVLGDLGWLAGHQGDYPA
                     ARTLLEQGLAFGQQNVLLQMVFDCPAVELGA"
BASE COUNT          560 a         1230 c         1068 g          625 t
ORIGIN      
        1 tccatttctt caaggtgcag gcgtttattg tgaccctgat gggcctcttc ctcgcgcggg
       61 gcctggcgta catcatcagc ctgacctccg tgaccatcaa cgacggcttc tacaagtatc
      121 tcgcgctcac acagattccg atgccgttta gccgccaagc atacgtgtat attccttcgc
      181 tggtcggccc gttgctgctg ctgctggcga tctatataac cttcttcacc cgctttgggc
      241 gcaccatcta tgccatcggc aataacgagc aatctgccag gctgatgggt ttaccggtcg
      301 gtcaaacgaa aatcctcgtc tattccttca gtggcttctg ctccgccctg gccggcattg
      361 tcttcagtat ctcgctactc gcgggctatg gacagttcgc cacagggatg gagctcgaca
      421 ccatcgccgc ggtggtgatg ggcggcacgt tgctgacggg cggcgtcggc aatgtcgttg
      481 gcaccctgtt cggcgtgcta atcgagggca cgatcatatc gatcctccaa tacaacggca
      541 ccttgagctc gtggtggacc aggattggcg ttggcgttct cacgctgatc tttatcggca
      601 ttcaaagtct gttctatatc cgaagaaagc actcctgaac gcgcgggcgc gatcggctac
      661 ctgctgaagg acagcacggc cgaggacttg tgcgcggcga tccccgtcgc ggtgcaggag
      721 atgctccgcg ctacggtccc ctctcacggt accaaaaccc acccgctcaa aagtgtggca
      781 gccgatcact cttttgaggg agcacaacca tccctcgaag ggggatggga tgctgcgtct
      841 gcatgctgct gcaaggcact tcggcggcgc cgtcaagggg tttttggcgg gcccggcgcg
      901 cgggcagatc cacgtggagc agttgccggg ctacgcgccg gacttgaacc ccgacgaagg
      961 ggtctggggt caactgaagt aggtggaatt gcggaacgtc acatgtcatg atcagactga
     1021 actccgccgc gagttgcgcc gggcaattgc ccgtctgcgc cacaagccgg acctggttcg
     1081 cggcttcatc aagcactccg gttattagat cttaatgcag cgatctgtag gctttccgtc
     1141 cccagacctg ggtgacaatg aacgtcagca ccgacgtgtc tggtcggctg gctatctcct
     1201 cacactccgc gatcgcgccg tccagatccg ctgctgtgag caacctggcg gcgagaatgc
     1261 gggggcgcag cgaggtcgca aactggatcg gcaaccgcat gtacgggtgc gcatcgtgca
     1321 aggcgattac ggccgcgcgc aggtccacct cgcgcagccc ggcatcccgc agcatcttgt
     1381 aggtccgctg ccccgcgttg aagtcgccac ccccaaggcg aaaggctgcc aggatcgtgg
     1441 tcttgagctg gttccaggcg gcagaagcgg gcaagcaggt ccagggcgtc gcatcgggct
     1501 cctggattac tagcaggccc cccggcttgg tgagccgcag catctccgcc agtagttctt
     1561 catcgcgccc caccggggca aacaggaagc gcacgtgaac caggtcgaac gcctcgcttg
     1621 ccagcccggt gttgtaggca tcggccttgc gaatctcgac gttcgtgaag ccgttgtctg
     1681 cgacgtaggc tcgcgcggcg gctaactgcg tatcgtcgac atcgacgcct acgactcgcc
     1741 cgtcagcccc gacccgcgta ctgagggggc cgaggatgcc catggccccg cagcccaaat
     1801 ccacaccggt ggcccccggt ggcaccccta tgcgatccag cagcgcctga gcttcaggct
     1861 cccagacgcg agcttgcagc cgcagccgct ctaactcggc ggcacctccc tggagtaagt
     1921 atccctgacc ctggttactc atcgaggccc tccttccaag cagcgcatca ccacacggat
     1981 ctggtcgttc ccgagccggt gtccacccgg gccggggcgc tgctgaccgt ggctgccgcg
     2041 cccggcgtgc cccctccgcg gatctgcgtg ccctggcgct gggtgccagg ccgctttgtg
     2101 cgctccggct tgcggcccca ccacctgcgg caggtgggcc ggctcacggc ccgcttgcac
     2161 gaccacgccc agggcttcca gcccccgccc ggcttcagcc gcccgcgcat cgacgacctt
     2221 cctgccacgc gggccgccgt gctgccgctg gtggccggga tccgcccgcc gcgcgacgtg
     2281 gcgctggtcg ccgctgtgct ggatcgcgtc gccgccacgc tggccgggct ggaccccggc
     2341 ccagacgctt acgggctgat ccactccgac ctgcaccagt ggaactacct gttccagcac
     2401 ggccaggtcc acctgctcga ctttgacgac tgcggctaag cccggttcct ctatgacctg
     2461 gccgtcacca cctttgaact ggagcatgac ccgcactttc ctgcggtgtg cgcggccctg
     2521 ctggccggct acgaggccac ccggcccctg ccgcccgccc ccgaaaccct gctgaccacg
     2581 ctcacccacc tgcggcacat ccagaacctg gtctgggaca tcggcctgca cggcgcccta
     2641 tacgggcgcg atcaccggca acagatcgtc gcgagcgagc tggacgggct gcgggccttc
     2701 ttgaccggct gaagcggcgc actccggcgc ccgcgggctg tgttaaaaac ccccgcggtc
     2761 agataagccg cctgcccccg cgcccccgcg ccgccctggc gcacgatgac cgggcgggcg
     2821 atcgccgggt gctcgtgcac cacgcggcca ccggagccgt gtgggatggg gtggcgcggc
     2881 atccccgctg ccttgccgcg ggcccgttac gcaccgagct ccaccgccgg gcagtcaaac
     2941 accatttgta gcagcacgtt ctgctggccg aacgccaggc cctgctccag cagcgttcga
     3001 gccgccgggt agtctccctg gtgcccggcc aaccagccca ggtcgccgag cacgtgggcg
     3061 acaagccgca cgtcacctag cttctggaac tgggtcaggg cgtcttccag cagcgcgcgc
     3121 gcgcctgtgt agtcacctcc cgccagggcc aggttcccca aggcgacgaa gcccatagct
     3181 atgttccgct ggtcacctag ctcacggaac tgggtcaggg actgttcgta caaggtccgg
     3241 gctgccgcta aatccccacg cccgtacccg gataccgcgt cgcccaggct gcggagcgcg
     3301 cgcgcgaccg tcaatagatc gccgtattcc cgcgccagag tcagactctg ctccagcaga
     3361 tctgaagcgg cggggaggtc tccttgctcc tgggccagaa acccggcccc gtagagagcc
     3421 cgcgcccgat cgcgccccgt cgtggccggg tccagcgagg ccgccgcctc cagcgctgta
     3481 gct
//
LOCUS       DASUYC010001116         2248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1809740, whole genome shotgun sequence.
ACCESSION   DASUYC010001116 DASUYC010000000
VERSION     DASUYC010001116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1809740"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1229)
                     /locus_tag="VF276_18210"
     CDS             complement(<1..1229)
                     /locus_tag="VF276_18210"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX6608840.1"
                     /translation="MRYRGTSPRVARGLITGLLAALLTGGAALWAAPAPHPGRVPAAQ
                     ATAPTGGFAGSATPAPGTTDVVETVPGEIVVRFKEQGGGRAMEPAGAQRALIAAGLGI
                     GAVVQRPLAVAGTYVVGVTPGRETDSAARLQKQPDVLAADPNLIRHVHALAVTPNDPL
                     YPVQWAYPRINAPAAWSTAQVPGVIVAVLDTGLDLNHPEFAGRIVPGVNYARPGASPQ
                     DDNGHGTHIAGIIAGGGNNGVGGAGLAWRASILPIKVADSGGNLTSEAWINGIAYAIQ
                     HRAQVINMSFGGTTYSGTEQAAINDAWRAGLVVVGSAGNSGDGGNPVEYPASYDNVVS
                     VAAIGSDNARAYYSEYNRFVDVTAPGGNARFSNDPPDRFILSSWPLGLQSNYQTGYGQ
                     EIGTSQAAAFVSGLAAL"
     gene            complement(1291..>2248)
                     /locus_tag="VF276_18215"
     CDS             complement(1291..>2248)
                     /locus_tag="VF276_18215"
                     /inference="COORDINATES: protein motif:HMM:NF019311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HEX6608841.1"
                     /translation="PNAQIDSVTGPQGGNALFFAVTVPPADRAAPYQVAVTIRDAAGA
                     QTVLTSTTGIDVDWSRAVPPGAQLPLQITWRGALRPPAWAVYHVRVDQSAGPADGAHL
                     ELAGRALDPAGSDVPLIAGRYPFTLTATVSRPAGVTRLLWTVPAGPTGTAPGEATVPP
                     AALLAPSLGEYGVTAIYRQGTDPAGPVSLVREEPAVNWFHFYTPFADTGPFVGEWRAT
                     LQAPQSGRYTFTADIGGTPGLWLDGAAVTWTPAGRSALNVPLYEATITLAAGPHTFRF
                     QDLAPGWREPCFLFWTPPGGTQQLIPAEVFRPELWPGEARSR"
BASE COUNT          303 a          799 c          819 g          327 t
ORIGIN      
        1 atgagggcgg ccagaccgga gacaaaggcc gccgcttgcg acgtgccgat ctcctggccg
       61 tagccggtct ggtagttgct ttgcaggccc agcggccagc tactgaggat gaagcggtcg
      121 ggcgggtcgt tgctgaagcg ggcgttgccg ccgggcgcgg tcacgtcgac gaagcggttg
      181 tactccgagt agtaagcgcg ggcattgtcg ctgccgatgg ccgccaccga gacaacattg
      241 tcgtacgagg cgggatactc caccgggttg ccgccgtcgc cggagttacc cgccgagccc
      301 accaccacca acccggcgcg ccaggcgtcg ttgatcgccg cctgctcggt gccgctgtac
      361 gtggtgccgc cgaagctcat gttgatgacc tgggcgcgat gctggatagc gtaggcgatg
      421 ccgttgatcc aggcttcgct ggtcaggttg ccgccgctgt cggccacttt gatcggcaag
      481 atggatgcgc gccaggccag gcccgcgccg ccgacgccgt tgttgccccc gcccgcgatg
      541 atcccggcga tgtgtgtgcc gtggccgtta tcgtcctgtg gcgaggcgcc gggccgggca
      601 tagttcacgc cgggcacaat gcgcccggca aactccgggt ggttcaggtc gaggccggtg
      661 tcgagcacgg ccacgatgac gccgggcacc tgggccgtgc tccaggcggc gggcgcgttg
      721 atgcgcggat aggcccactg caccgggtag agcgggtcgt ttggggtgac ggccagggcg
      781 tggacatggc ggatcagatt ggggtcggcg gcgagcacgt cgggctgctt ttgcaaccgg
      841 gccgccgagt ccgtctctcg accaggggtg acgccgacaa cataagtacc ggccaccgcc
      901 agcgggcgct gcacgacagc cccgatgccc agccccgcgg cgatcagcgc gcgctgcgcg
      961 cccgccggct ccatggcgcg cccgccgccc tgctccttga agcgcaccac gatctcgccc
     1021 ggcacggttt cgaccacatc ggttgtgccg ggggctggcg tggcgctgcc cgcgaacccg
     1081 ccggtcggcg ctgtggcctg ggcggcgggc accctgccgg gatgcggcgc gggcgcggcc
     1141 cacaacgcgg cgccgccggt gagcagcgcg gccaatagcc cggtgatcaa cccgcgagcc
     1201 acgcgcgggg aggtgcctcg gtagcgcatt gttggtctcc tgggacgggg cggcgcgccg
     1261 ttaccgcgca ccgggccggt ccgccggctc ctaccggctg cgagcttcgc cgggccacaa
     1321 ttcgggccgg aatacctccg ccgggatcaa ttgctgcgtg ccgcccggcg gcgtccagaa
     1381 tagaaaacag ggttcgcgcc agcccggcgc gaggtcctgg aaccggaatg tgtgcggccc
     1441 cgcggccagc gtgatcgtcg cttcgtacag cggcacgttg agcgcgctgc gcccggcggg
     1501 cgtccaggtg acggcggccc cgtcgagcca cagccccggc gtgccgccga tgtccgccgt
     1561 gaaggtatag cgcccgcttt gcggcgcctg caaggtggcg cgccactcgc ccacaaacgg
     1621 ccccgtgtcc gcgaacggcg tatagaaatg gaaccagttc accgccggct cctcgcgcac
     1681 caggctcacc ggccccgccg gatcggtgcc ctggcgatag atcgccgtga cgccgtactc
     1741 gcccagcgac ggcgccagca gcgccgccgg gggcaccgtt gcctcgcctg gcgccgtgcc
     1801 ggtggggcct gcgggtaccg tccagagcag ccgcgtcacg ccggccggcc ggctcaccgt
     1861 cgccgtcagg gtgaacgggt agcgcccggc gatcagcggc acatcggacc ccgccggatc
     1921 gagcgcccgc ccagccagtt ccaggtgcgc gccgtccgcc ggtccggcgc tctggtccac
     1981 gcgcacatga tatactgccc aggccggtgg gcgcaaggcc cctcgccagg tgatttgcaa
     2041 cggcagttgc gcgcccggcg gcaccgcgcg actccaatcc acgtcgatcc cggtcgtgct
     2101 ggtcagcacc gtctgcgcgc ccgcggcgtc gcggatagtc acggccacct ggtagggcgc
     2161 ggcgcggtcc gcgggcggca cggtgacggc gaagaagagc gcgttgccgc cctgcggccc
     2221 ggtcacgctg tcgatttgcg cgttcgga
//
LOCUS       DASUYC010001117         2050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_5780,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001117 DASUYC010000000
VERSION     DASUYC010001117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5780"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1504
                     /locus_tag="VF276_18220"
     CDS             <1..1504
                     /locus_tag="VF276_18220"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX6608842.1"
                     /translation="AAGAGGGATPPAASSFPTTVPPASGFPTTVPPASGFPTPAATNP
                     PAARGFPTPAATNPPAARSFPPPAGAERVGRVNFRDDKQLTDQVVVTINAAPAKPAGQ
                     ALYAWLLASGTGAHANLGRLEADTSAPVTLRYSDPQNTNLIASYDRFEVTAEALEVVP
                     NAPSGDVVLSGQLPPQALVHIRHLLVSFPDTPNKIGLEVGLREQVNVMLQHAQFMRDA
                     QAAGKLAEVKLHAEHLVNLIEGSQGAHYGDLNKDGQITNPGDGYGLLPNGSQLGYLQG
                     AKDHAELSAAAPDATADIKLHAGHVAITVDNVTGWVTPIRDHALAVINAPDLNTTAPL
                     ARDILTLAQQSFNGVDLQGDGQILPIPGSGGALTSYQHAQLMAAIPLQAGNVSFPAGP
                     APAAPTATTQAQAPAAAPAANAVQVSIRNFLFGTGPLTVKVGTTVTWVNDDSAAHTAT
                     ADNKLWDSGVLQKGQSFSFTFKQAGQFPYYCTLHGGPGGQGMASSITVEP"
     gene            1539..>2050
                     /locus_tag="VF276_18225"
     CDS             1539..>2050
                     /locus_tag="VF276_18225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608843.1"
                     /translation="MLNFNDGTLHVEKARVLMGGWGLLILLASIVNQFFDLPNPNYVL
                     LLWTGVTVLGILAHITSLIHGLGQNFGAWVIVIVIGWAFTFYVVKFDNGTHIDLYGDL
                     AGVWLILLGIGYIPTAYHVERHFLVFAALHIVAGVLLELSARGVMRIGFLDAYSTLIF
                     GLVAGLPLLIA"
BASE COUNT          350 a          742 c          645 g          313 t
ORIGIN      
        1 ggcggcggga gccggcggcg gggccacccc gcccgccgcc agcagcttcc ccacgaccgt
       61 cccgccggcc agcggtttcc cgacgaccgt cccgccggct agcggcttcc cgaccccggc
      121 agccacgaac ccgcccgccg cccgcggctt ccccaccccg gctgccacga atccacccgc
      181 cgcccgcagc ttcccccccc ccgcgggagc cgagcgggtg ggccgcgtca acttccgcga
      241 cgacaagcaa ctgacggacc aggtggtggt gacgatcaac gcggccccgg cgaagccggc
      301 gggccaggcg ctttacgcgt ggctgctggc tagcggcacc ggtgcgcacg ccaatctggg
      361 ccggctggag gccgacacca gcgcgccggt cacgctgcgc tatagcgatc cccaaaacac
      421 caacctgatc gcctcctacg accggttcga ggtcacggcg gaagcgctgg aagtcgtgcc
      481 gaacgcccca tccggcgacg tggtgctgag cggccagttg ccaccgcagg cgctcgtcca
      541 catccgccat ctgctggtga gtttccccga tacgccgaac aaaatcggcc ttgaagtggg
      601 cctgcgcgag caggtgaacg tgatgctcca gcacgcccaa ttcatgcgcg acgcccaggc
      661 cgccgggaag ctggccgagg tgaaactgca cgccgaacac ctggtcaacc tgatcgaggg
      721 atcgcagggc gcgcactacg gtgacctgaa caaggacggg cagatcacca atccgggcga
      781 cggctacggg ctgctgccca acggcagcca gttggggtat ctgcaaggcg ccaaggacca
      841 cgccgagttg tccgccgccg cgcccgacgc caccgccgac atcaagctgc acgccgggca
      901 tgtggcgatc accgtggata atgtgaccgg ctgggtgacg ccgatccgcg accacgcgct
      961 ggcggtcatc aacgcgcccg acctcaatac caccgcgccc ctcgcccgcg acatcctgac
     1021 gctggcccag cagagcttta acggcgtgga cctgcaaggc gacggccaga tcctgcccat
     1081 tccgggcagt ggcggggcct tgacgagcta tcaacatgcc caactaatgg cggccattcc
     1141 gctgcaggcc ggcaatgtga gcttccccgc cggcccggcg cccgccgccc ccaccgccac
     1201 cacgcaggcc caggcgcccg ccgccgcgcc tgccgccaac gcggtccagg tctcgatccg
     1261 caacttcctg ttcggcaccg gcccgctcac ggtgaaagtc gggacgacgg tcacctgggt
     1321 caacgacgac agcgccgccc acacggccac ggcggacaac aagctctggg acagcggggt
     1381 gctgcaaaaa gggcaaagct tcagcttcac cttcaagcaa gccgggcagt tcccgtatta
     1441 ttgcacgctg cacggcggcc ccggcgggca gggcatggca tccagcatta cggtcgagcc
     1501 gtgagcacgc cgcagcttag aaacggagga caaacacgat gctgaacttc aatgacggca
     1561 cgctgcatgt cgagaaagca cgggtgctga tgggcgggtg gggcctgctg atcctgctgg
     1621 cgtccatcgt caaccagttc tttgacctgc ccaacccgaa ttatgtgctg ctgctctgga
     1681 ccggggtgac ggtgctgggg attctcgccc atataacgtc gctgatccac ggcctgggcc
     1741 agaacttcgg cgcctgggtg atcgtcatcg tgatcggctg ggcgttcacg ttctatgtcg
     1801 tcaagttcga taacggcacg cacatcgacc tgtacggcga cctggccggc gtctggttga
     1861 tcctcctggg cattggctac atccccaccg cctatcacgt ggagcggcac ttcctggtct
     1921 tcgccgcgct gcatatcgtc gccggtgtgc tgttggagct ttcggcccgc ggggtgatgc
     1981 gcatcggctt cctggacgcg tactccacgc tcattttcgg cctggtcgcc gggctgccgc
     2041 tgctgatcgc
//
LOCUS       DASUYC010001118         3277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_824975,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001118 DASUYC010000000
VERSION     DASUYC010001118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824975"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..940
                     /locus_tag="VF276_18230"
                     /pseudo
     CDS             1..940
                     /locus_tag="VF276_18230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020467591.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(998..1636)
                     /locus_tag="VF276_18235"
     CDS             complement(998..1636)
                     /locus_tag="VF276_18235"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6608844.1"
                     /translation="MFEVCPACGLYAVEKTIDPGGPFAICPHCGHAQPFLRLPLFVVT
                     GASGAGKTTACLAVVGQLPECVVLDSDVLWDARFNTPEDNYRAYRALWLTLARDIGQN
                     GRPVVLFGSAVPEQFEGLPARRYFSAIHYLALVCEDTVLVERLQSRPAWREAGKPEFI
                     AAMLAFNRWFYEHAATTQPPITLHDTEAGSLAATAAFIIAWIRAHLAALEAK"
     gene            complement(1657..2916)
                     /locus_tag="VF276_18240"
     CDS             complement(1657..2916)
                     /locus_tag="VF276_18240"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6608845.1"
                     /translation="MPKPVPSRPLRLAFIAPFGLRPKGTASARALPLAQALVARGHAV
                     RLIVPPWDDPSAPPEGRVSCLGGVEVVELPAPRPGVPGAGVIGWPTALVREALRGRPD
                     VVHVFKPKAYAGFAALGLAALRRPWVLDTDDWEGRGGWNALNRYPAPQRALFQWQETT
                     LPHLAGAATVASRTLETQLWGFGVPRGRVFYLPNGVDHAKYDPWLAAAADPAAVERVR
                     GLYGLPPATPDNPLLLLWTRFVEFPVEWPLRVLEALAPRVPGLRLLVVGEGFHGEERR
                     LQEAADARGLAGRVLVCGRVEGVDLGALLAQGTVALYPMRDTLVNRAKCPVKLLDLLA
                     LGVPVAAHNVGQVGEYLGHGESGVLAPPGDVPGLAAGVQALLADPARREALGRAAAAR
                     AWTCFPWSRLATAAEAAYAVALRRAGR"
     gene            complement(2897..>3277)
                     /locus_tag="VF276_18245"
     CDS             complement(2897..>3277)
                     /locus_tag="VF276_18245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6608846.1"
                     /translation="PLEMLREAQPMYEQQAIGFLGRFLFTYEQCRRPIRTMSGGEKAR
                     LQLARLMLSGANCLLLDEPTNNLDILSAEVLERALEDYTGAVVVISHDRYFLDHVVDR
                     IWALEDGRLHEYLGGWSAYAEARA"
BASE COUNT          573 a         1113 c         1123 g          468 t
ORIGIN      
        1 cgggccgcca tatgaaacaa tctatgccca tcaaatccag ttgtcgcgcg gcaaagggca
       61 aactgcctat taccaacgcc agggccaata atttgcagaa cgtcagcgtc gatattccca
      121 cgggggttct gacagtcgtg acgggggtgg caggttccgg caaaagttcg ctgatccact
      181 actcgtttct gcctcaatgc cctgacgcca tcgtgatcga tcaatcggtg gtgggcgcgt
      241 cgagccgatc gaatcccgcg acctataccg ggatcatgga cgacatccgc aaagcgttcg
      301 cgacggcaaa caaggtcagc ccgtcgttgt tcagtttcaa ttcgaaaggc gcctgcgaaa
      361 cctgccaggg cctgggcgtg atctacacta atctgggctt catagacgaa gtgaaaatcc
      421 cctgcgagac gtgcggcggc aagcgcttca aggatgatgt gccggcctac acgctcgacg
      481 gcaaatcgat ctccgatgtg ctggcgatgt cggtgcgcga ggcgctggga ttcttcaccg
      541 ccaaagaggt cgtgcgccgc ttgcaggcga tgagtgatgt cgggttggat tacctgacgc
      601 tgggccagcc actcagcacg ttatcgggcg gggaaagcca acggataaag ctcgccagcg
      661 aactgcataa aaagggcagc atctacgtga tggacgagcc gaccaccggc ttgcacatgt
      721 cggatatcgg ccatttactg gcgatcatgg accggctggt cgatacgggc aacactgtga
      781 tcgtcatcga acacaacctg gatattatca agaacgccga ctggatcatc gatatggggc
      841 cggaaggcgg caacaaaggc gacaaggtga tgttcgaggg gacgccgcgg caattgctcg
      901 atgcgcccaa tcgctcacca gcgcctactt gcggggttga tccgataggc tgattagaag
      961 ggacacccca tgtggggtcc tggtcaagcg cccgcgctca tttagcttcc agtgcggcca
     1021 ggtgcgcgcg aatccaggcg atgatgaacg cggccgtcgc ggccagggag ccggcctcgg
     1081 tgtcgtggag cgtgatcggg ggctgggtcg tggccgcgtg ctcgtagaac cagcggttga
     1141 aagccagcat ggcggcgatg aactccggct tgccggcctc gcgccaggcc gggcggcttt
     1201 gtaggcgctc aacaagcacc gtatcctcgc agacgagtgc caggtagtgg atggccgaga
     1261 agtagcggcg agcgggtagg ccctcaaact gctccggcac ggcgctgccg aacagcacca
     1321 ccgggcgccc gttctggccg atgtcgcgcg ccagggtgag ccagagggcg cggtaggcgc
     1381 ggtagttgtc ctccggggta ttgaagcgtg catcccaaag aacatcgctg tccagcacca
     1441 cgcattcggg aagctgcccg acgacggcga ggcaggcggt cgtcttgccc gcgccgctgg
     1501 cgccggtgac gacgaacagc ggcaggcgca agaagggctg cgcgtggccg caatgcgggc
     1561 agatcgcgaa cggtcccccc ggatcgatgg ttttctccac cgcatagagc ccgcaagccg
     1621 ggcaaacctc gaacattggg cgccgtcctc tgccgctcag cggcctgcgc ggcgtagggc
     1681 aacggcgtag gcggcttcgg cggcggtggc gaggcgcgac caggggaagc aagtccaggc
     1741 acgggcagcg gcggcccgcc ccagcgcttc gcggcgcgcc gggtcggcca ggagcgcctg
     1801 gacacccgcg gccagccccg gcacatcgcc cggcggcgcc agcaccccgc tctcgccgtg
     1861 ccccaggtac tcgcccacct gccccacgtt gtgcgcggcc accggcacgc cgagcgccag
     1921 cagatcgagc aacttgacgg ggcacttggc ccggttcacc agcgtgtcgc gcatgggata
     1981 gagcgccacg gtgccttgcg ccagcagcgc gcccaggtcg acgccctcca cgcgcccgca
     2041 gaccagcacc cgcccggcca acccccgcgc atccgccgcc tcttgcagcc ggcgctcctc
     2101 gccgtgaaac ccctcgccca cgacgagcag gcgcagcccc ggcacgcgcg gcgccagcgc
     2161 ctccagcacg cgcagcggcc actcgacggg gaactcgacg aagcgcgtcc agagcagcag
     2221 cagcgggttg tccggcgtgg ccgggggcaa gccgtacagg ccgcgcaccc gctcaacggc
     2281 ggcggggtcc gcagcggcgg ccagccaggg gtcgtacttg gcgtgatcca cgccgttggg
     2341 caggtagaag acccgcccgc gcggcacgcc gaagccccag agctgcgtct ccagggtgcg
     2401 gctcgccacg gtggccgccc cggccaggtg cggcagcgtg gtctcctgcc attggaacag
     2461 ggcgcgctgc ggcgccgggt agcggttgag cgcgttccag ccgccccggc cctcccagtc
     2521 gtcggtgtcg agcacccagg gccggcgcag cgccgccagg cccagcgcgg cgaacccggc
     2581 gtaggccttg ggcttgaaga cgtggaccac gtcgggccgc ccgcgcagcg cctcgcgcac
     2641 cagcgcggta ggccagccga tcaccccggc gccgggcacg ccgggccggg gcgccggtag
     2701 ctcgaccacc tcgacgccgc ccaggcacga gacgcgcccc tccggcggcg cgctcggatc
     2761 gtcccacggc ggcacgatca ggcgcactgc gtggccgcgg gcgaccagcg cctgagccag
     2821 cggcaaggcc cgcgcgctag ccgtgccctt gggccgcagc ccgaacggcg cgatgaaggc
     2881 caggcgcagg ggccggctag gcacgggctt cggcataggc gctccagccc cccaggtatt
     2941 cgtgcagacg cccgtcctcc agcgcccaga tgcggtccac cacgtggtcc aggaagtagc
     3001 ggtcgtgcga gatgacgacc acggccccgg tgtagtcttc cagcgcgcgc tccagcactt
     3061 cggcggagag gatgtcgagg ttattggtcg gctcgtccag cagcaggcag ttggcgcctg
     3121 acagcatcag ccgggccagt tgcaggcgcg ctttctcgcc gccgctcatg gtgcgaatgg
     3181 gccggcggca ctgctcgtag gtgaacagga agcgccccag gaagccgatc gcctgctgct
     3241 cgtacatcgg ctgggcctcg cgcagcatct ccagcgg
//
LOCUS       DASUYC010001119         5280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1347314, whole genome shotgun sequence.
ACCESSION   DASUYC010001119 DASUYC010000000
VERSION     DASUYC010001119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5280
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1347314"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="VF276_18250"
     CDS             complement(<1..745)
                     /locus_tag="VF276_18250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEX6608847.1"
                     /translation="MNRVMRGSNFRGLRLGIAGVVALGLAMILAGHLAWGAPAPDTMN
                     RPIPDAGAGIAAAERAAPNVPTTVQQFEDVLPSNPFYDFINNLYLDHIISGYTCGGVG
                     EPCVAPGNRPYYRPNNNVTRGQMGKFVDLGRRNIADAVGTRLVITNSAQIGMVISSTT
                     TDSLDVNNASGAEAVQVLCLTPDTNCWAVYSSAPAGDYAGVFNGGRGVSVSSNDSGYA
                     ALHATASGTGSNSYAVLGTSAYRAGYFKST"
     gene            1236..3080
                     /gene="typA"
                     /locus_tag="VF276_18255"
     CDS             1236..3080
                     /gene="typA"
                     /locus_tag="VF276_18255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEX6608848.1"
                     /translation="MQRKDIRNIAIVAHVDHGKTTLVDAMLKQSHIFRENQQVAERVM
                     DSNALERERGITILAKNTSIVYKDVKINIVDTPGHADFGGEVERVMNMVDGVLLLVDA
                     VDGPMPQTKFVLRQALQRGHKAIVVVNKIDRANARPMHTINATFDLFVDLGANEEQAE
                     FPVLFTNALTGEAGLDHNHMGPTLEPLFETILEHIPGPEVEPDQPTQLLVTNLSNDEY
                     KGKIVVGRLQSGTVRKGQPVARIDHDGNITPAKVTQVFNYHGLTRQEVEEVAAGDIIA
                     LAGVPDANIGDTISDLANPVALPPIRVEEPTLRMSFSVNNSPFAGREGTYVTSRKLRE
                     RLYNELEHDVALRVEDTDTTDTFLVSGRGELHLTILIENMRREGYEFQVSKPEVIYKE
                     QDGKLQEPYEQVEIEVAQEVLGVVVEMLGARRGVMQDMKYRDDGSVHLVSRVPTRGLL
                     GFRQQFLTNTRGQGIMNTLYAGYGPLAGPVATRNFGSLIAWEAGPTTTFGLHGAQERG
                     QLFLGPGVEVYEGMVVGQHIREKDLEVNVCKKKHLTNMRSSTAEEALRLEPPRIMSLD
                     DAIEYLADDELLEVTPKAFRLRKRVLSHQDRGRLAGQAKKGVPAGVSS"
     gene            3203..3751
                     /locus_tag="VF276_18260"
     CDS             3203..3751
                     /locus_tag="VF276_18260"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HEX6608849.1"
                     /translation="MDNQQVLQKYLGDMHALEAHIQTAIDKQVKLAENDPEAKQKFAE
                     MSGTLKGHLSALEQRLEALGGSATSPVKEAGAAVLGLGAGLIDKMRAEEISKDLRDDY
                     TALSHSIISYVMLNATALALGDQQTADLAERNVKDNVAFVVWVNNNMPKFVIKDLQDS
                     GASIDQGAAEASQAAAKRIWNQ"
     gene            3883..4659
                     /locus_tag="VF276_18265"
     CDS             3883..4659
                     /locus_tag="VF276_18265"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6608850.1"
                     /translation="MDSAPNGQPPTTQEARAAMQFAEGDAAFAQGDLAGAAVAYEAAA
                     ADFRALDAPKLLADCLHQLGVIRQYQDDYAGAEPLLQESLSIAERLDDAEAQARTLHQ
                     LGRSRVQQGAYAAADDYYSRSLSLKTMLGDRAAMGSTLHQMGMVRRLMGNLHEAAEFY
                     GLALAINQRLQDWNGVGGTLAELGRLRLQEGDLAAALANSWQALSIFHGTSRPEAAYA
                     AITILDVRRRMEPAAFAEAAGRAGITHEVIDQVEEAVHER"
     gene            4649..>5280
                     /locus_tag="VF276_18270"
     CDS             4649..>5280
                     /locus_tag="VF276_18270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEX6608851.1"
                     /translation="MNAEIVSVGTELLLGNITDTNATYLTRELAALGINCYWVSQVGD
                     NLGRLTEVLGRAMDRSDLTVVSGGLGPTEDDLTREAIAAVLGEEMVVQPDLEAELRAF
                     FAHRGQVMPARNAKQATLIPSAQVLANPVGTAPGWWVEHNGHVLVAMPGVPHEMMRMW
                     SQEIAPRLVAGGNGEIISRTLKVLGLGESAAEERVEDLLRSNNPTLATYA"
BASE COUNT         1028 a         1714 c         1752 g          786 t
ORIGIN      
        1 tggtggactt gaagtagccg gcacggtagg cggaggtacc gaggaccgca taggaattcg
       61 accccgtccc cgacgcagtc gcatggagag ccgcatagcc gctgtcgttg ctggagacgc
      121 taacgccgcg ccccccattg aagacaccgg cgtagtcgcc cgccggcgcg ctgctataca
      181 cggcccaaca gtttgtgtca ggggtgaggc acaacacctg gacggcctcc gcgcccgagg
      241 cgttattgac gtcgagactg tcggtcgtgg tcgaggagat gaccatcccg atctgcgcac
      301 tattggtgat gaccagccgg gtgccgacgg cgtcggcgat gttgcgccgg cccaggtcga
      361 cgaacttgcc catctgtccg cgggtgacat tgttgttcgg gcggtagtag gggcggttgc
      421 cgggcgccac gcacggctcc cccacccccc cgcaggtata gccgctgatg atgtggtcga
      481 ggtagaggtt gttgataaag tcgtagaagg gattgctggg cagcacatct tcaaattgct
      541 gcaccgtggt ggggacgttg ggcgccgccc gttcggcggc agctattccc gcgccggcat
      601 ccgggatcgg acggttcatg gtgtcagggg cgggtgcgcc ccaggccaga tggccggcca
      661 agatcattgc caagcccaga gcaaccacgc cggcgatccc cagccggagc ccgcgaaagt
      721 tcgatccacg catgacgcga ttcatcggta ctgctccctt cctaccactt gcgttccgtc
      781 ctggtgccta tcggtggtgc gctatctgtg gcagcgccag ggcggaggtc aagaccgcgc
      841 gttccgcgcg cctgccgcca cagtaagccc attatagagg cggcaacgac aaagcacaat
      901 caatccacgt aaaatcggcc tgaatgcagc ggcacaagcc gccgctcccc cgcgccggcg
      961 acaaagatag gacggaccga tagcgtgtag cgtgggtgcg ggggagcggc agggtcgagc
     1021 gaggtggctt atcgcgggat gtactcccgc gagggtgcca atcagcaaaa atccgccgaa
     1081 tcgggtataa ttagtagctg tgcgtggagc atgcgcgtgc gtaacctcta caaaacatcc
     1141 actgattatt tgccgggcat ggacggcgcg actcccgccc atcggacgca gcaggcagac
     1201 cagggtcgcc gctagtatat tagggtagga gtctcatgca gcgcaaagat atacggaaca
     1261 tcgccatcgt ggcccacgtc gaccacggca agaccacgct cgtggacgcg atgttgaagc
     1321 agagccatat tttccgcgag aaccagcagg tcgcggagcg agtcatggac tcgaacgcgc
     1381 tcgaacgcga gcgcggcatc accattctcg ccaagaacac gtccatcgtc tataaggatg
     1441 tcaagatcaa catcgtggac acgcccggcc atgccgactt cggcggcgag gtggagcgcg
     1501 tgatgaacat ggtcgacggc gtgctgctgc tggtcgacgc cgtggacggc cccatgccgc
     1561 agaccaagtt cgtgctgcgc caggcgttgc agcgcgggca caaggccatc gtcgtcgtca
     1621 ataagatcga ccgggccaac gcccgcccca tgcacacgat caacgccacc ttcgacctgt
     1681 tcgtggacct gggcgcgaac gaggagcagg ccgagttccc ggtgctgttc accaacgcac
     1741 tgaccggcga ggccgggctg gaccacaacc acatggggcc gacgctggag ccgctgttcg
     1801 agacgatcct ggagcacatt ccgggaccgg aggtcgagcc cgaccagcca acccaactgc
     1861 tggtgaccaa cctcagcaac gacgaataca agggcaagat cgtcgtgggc cggctgcaga
     1921 gcggcacggt gcgcaagggc cagccggtgg cgcgcatcga ccacgacggc aacatcacgc
     1981 ccgccaaagt cacccaggtg ttcaactacc acggcctgac ccgccaggag gtcgaggaag
     2041 tagccgccgg ggacatcatc gccctggccg gcgtgcccga cgccaacatc ggcgacacga
     2101 tcagcgacct ggcgaacccg gtggcgctgc cgcccatccg ggtcgaggag ccgacgctgc
     2161 gcatgagctt cagcgtcaac aacagcccct tcgccgggcg cgagggcacc tacgtcacca
     2221 gccgcaagct gcgcgagcgg ctctacaacg agttggagca cgacgtggcc ttgcgcgtgg
     2281 aggatacgga cacgacagac accttcctgg tctcgggccg cggcgagttg cacctgacca
     2341 tcctgatcga gaacatgcgc cgcgagggct acgagttcca ggtctccaag ccggaggtca
     2401 tttacaagga acaggatggc aagttgcaag agccgtatga gcaggtcgag atcgaagtcg
     2461 cccaggaagt gctgggcgtg gtggtcgaaa tgctcggcgc gcggcgcggg gtcatgcaag
     2521 acatgaagta ccgcgacgac ggcagcgtgc acctggtgtc ccgcgtgccg acgcgcggcc
     2581 tgctcggctt ccgccagcag ttcctgacca acacacgcgg ccagggcatc atgaacacgc
     2641 tctacgccgg gtacgggccg ctggccgggc cggtcgccac gcgcaacttc ggctcgctga
     2701 tcgcctggga ggccggaccc accaccacct tcggcctgca tggcgcccag gagcgcggcc
     2761 agctcttcct cggccccggc gtggaggtct acgagggcat ggtcgtgggt cagcatatcc
     2821 gcgagaagga cctcgaagtc aacgtctgca agaagaagca cctgaccaac atgcgcagtt
     2881 cgaccgccga ggaagcgctg cgcctggagc cgccgcgcat catgtcgctg gacgatgcca
     2941 tcgagtacct ggccgacgac gaattgctgg aagtcacgcc gaaagccttc cgcctgcgca
     3001 agcgcgtgct gtcgcaccag gaccgcgggc ggctggcggg ccaggccaag aaaggcgtgc
     3061 cggccggcgt cagcagttaa gccatttgtc gatctggcat acatattgct tttctatctc
     3121 tcgcctacat gatgacgccc gtctatcttt accggggtag acggcgatga agcaagaaag
     3181 ttagaggaga gatagattag ttatggataa tcagcaggtt ttgcagaagt atcttggcga
     3241 catgcacgcg ctggaggcgc atatccagac cgccatcgac aagcaggtga agctggccga
     3301 gaacgacccc gaggccaagc agaagttcgc cgagatgtcg ggcacgctca agggccacct
     3361 gtcggcgctg gagcagcggc tggaagcgct cggcggcagc gccaccagcc cggtgaagga
     3421 agcgggcgcg gcggtgctcg gcctgggcgc cgggctgatc gacaagatgc gcgccgagga
     3481 gatctccaag gatctccgcg atgactacac cgcgctgagc cacagcatca tcagctacgt
     3541 catgctgaac gccacggcgc tggcgctggg cgaccagcag acggcggacc tggccgagcg
     3601 caacgtcaag gacaacgtcg ccttcgtcgt ctgggtcaac aacaacatgc cgaagtttgt
     3661 gatcaaggac ttgcaggaca gcggcgcgag catcgatcag ggtgccgccg aggccagcca
     3721 ggcggcggcc aagcgcatct ggaaccaata atcgccgtag cacctcacga ctcgaccgca
     3781 ggccggcgcc ccaggggtgc cggccttttg cgtgtgtttg acaaaacgtg ccccgccgcc
     3841 tacaatcgag cgagagacca cgcactagga cggagcgaaa ccgtggattc agcaccgaac
     3901 gggcaaccgc ccaccaccca ggaggcccgc gcggcgatgc agtttgccga gggcgatgcc
     3961 gccttcgcgc agggcgatct ggcaggcgcc gccgtggcct acgaggccgc cgccgccgat
     4021 tttcgcgccc tggatgcgcc caaactgctg gccgattgcc tgcaccagct cggcgtcatc
     4081 cgccagtacc aggacgacta cgccggggcc gagccgttgt tgcaggagag cctgagcatc
     4141 gccgagcggc tggacgacgc cgaggcccag gcgcgcacgc tgcaccagtt ggggcgctcg
     4201 cgggtgcagc agggcgccta tgccgccgcc gacgactact atagccgcag cctatcgctc
     4261 aagacaatgc tgggcgaccg ggcggccatg ggcagcacat tgcaccagat gggcatggtg
     4321 cgccgcctga tgggcaacct gcacgaggcc gccgagttct acggcctggc gctcgccatc
     4381 aaccagcggc tccaggactg gaacggcgtc ggcggcacgc tggccgaact gggccgcctg
     4441 cggctgcaag agggcgatct ggcggcggcg ctcgccaact cctggcaggc gctctcgatc
     4501 ttccacggca ccagccgccc cgaagccgcc tacgctgcga tcaccattct cgacgtgcgc
     4561 cgccgcatgg agcccgccgc cttcgcggaa gctgcgggcc gcgccggcat cacccacgaa
     4621 gtgatcgacc aggtggaaga ggctgtccat gaacgctgag atcgtctcgg tcggcaccga
     4681 gctattgctg ggcaacatca ccgacaccaa cgcgacctac ctgacccgcg aactggccgc
     4741 gctcggcatc aactgctact gggtctcgca ggtgggcgac aacctgggcc gcctgaccga
     4801 agtgctgggc cgggcaatgg accgctcgga cctcaccgtg gtcagcggcg ggctgggccc
     4861 caccgaagac gacctgacgc gcgaggccat cgccgccgtg ctgggcgagg agatggtggt
     4921 gcagcccgac ctggaggccg aattgcgggc cttcttcgcc caccggggcc aggtgatgcc
     4981 cgcgcgcaac gcgaaacagg cgacgctgat cccctcggcc caggtgctgg cgaaccccgt
     5041 cggcacggcg cccggctggt gggtcgagca caacggccat gtcctggtgg cgatgcccgg
     5101 cgtgccccac gagatgatgc gcatgtggag ccaggagatc gcgccgcgcc tggtcgcggg
     5161 cggcaacggc gagatcatct cgcggacgct gaaggtgctg ggcctgggcg agtcggcggc
     5221 ggaagagcgc gtcgaggacc tgttgcgcag caataacccc acgctagcga cctatgccaa
//
LOCUS       DASUYC010001120         3141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_244899,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001120 DASUYC010000000
VERSION     DASUYC010001120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244899"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VF276_18275"
     CDS             complement(<1..267)
                     /locus_tag="VF276_18275"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX6608852.1"
                     /translation="MSAPPPASSLRRPYLLTLGDLLLDVLVEGDFRREYDSAGTVQLY
                     PGGSAANFAAGAQALGARVRFVGRVGDDAAGRLLIHDMQDRGIAA"
     gene            complement(264..1202)
                     /locus_tag="VF276_18280"
     CDS             complement(264..1202)
                     /locus_tag="VF276_18280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HEX6608853.1"
                     /translation="MSTAFAQNDPDTPFVIAPEVAAAFAAGRAVVALESTVIAHGLPR
                     PTNLETARACEDAVRQAGAVPATIAVFDGRVHIGCDAAALERLATDPNIAKLSVRDLP
                     MALALRRNGATTVASSIYLARACPHPIPVFATGGIGGVHRGWQQTLDISADLPVLAAS
                     DIVTVCAGAKSILDLPATREWLETHAVSLLGWQTDEFPAFYTRQSGLSVDARVEDAAQ
                     VAAIVRARHALRMPGGVLLTAPLPAEAAIPAAEIRHAIDDALQAAAEIGVKGKELTPF
                     LLGHLHRKTEGRSLRANVALLLNNAAIAGQVAVALA"
     gene            1448..2608
                     /locus_tag="VF276_18285"
     CDS             1448..2608
                     /locus_tag="VF276_18285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX6608854.1"
                     /translation="MDFELSEEHRLFRESVRDFAEKEVAPGAIERDRTHEFPADLLKR
                     MAELGLLGIPFPEKYGGAGGDTLSYAIAVEEISRVDGSLGIILAAHISLGASPFYLFG
                     TEEQKKRWLVPLASGEMLGAFGLTEPGAGSDAGGTTTTAVREGDEWVINGRKLWITSG
                     RVAGVIVATAKTDRHAQGSRGISSFIIPRGTPGLSYGKDEDKMGLRSSITSPLFFENV
                     RVPAGNILGKEGDGFKQFMQILDGGRISIGAMGLGLGQGAFEAALSYATRREQFGQPI
                     ANFQAIQFKLADMAMKLEAARLLVYKAAMLKDQKKPFTQAAAMAKLYASEIGEECAYQ
                     AIQIHGGMGYAVESQVERMYRDVRLTTIGEGTSEIQRMVIARNLLKEAARTA"
     gene            2617..>3141
                     /locus_tag="VF276_18290"
     CDS             2617..>3141
                     /locus_tag="VF276_18290"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6608855.1"
                     /translation="MDLDLTEEQAQIRQLAREFAERELAPHALEWDETEHFPREVFAQ
                     MGALGFAGMLISEEYSGVGADTLSYILMLEEINKVLPALGTVVSVHNSLVGHAIARWG
                     NAEQRQEFLPRLATGEWLGGYALSEAHAGSDPASMRTLAVRDGDEYHITGTKTWITSG
                     QSADVFILFAVTDRK"
BASE COUNT          539 a         1053 c         1102 g          447 t
ORIGIN      
        1 ggcggcgatg ccccgatcct gcatgtcatg gatcagcagg cggcccgccg cgtcgtcgcc
       61 gacgcgcccc acaaagcgca cccgcgcccc cagcgcctgg gcgcccgcgg cgaaattggc
      121 tgccgagccg cccggataga gctggaccgt gcccgcgctg tcatactcgc ggcggaaatc
      181 gccctccacc agcacgtcga gcagcaggtc gccgagcgtc agcagatagg ggcggcggag
      241 ggaggaggcg ggtggaggcg cgctcacgcc agggcaacgg cgacctggcc ggcgatggcg
      301 gcgttgttca gcaacaacgc gacgttggcg cgcaggctgc ggccttcggt cttgcggtgc
      361 agatggccga gcaggaacgg cgtcagttcc ttgcctttca cgccgatctc cgccgcggcc
      421 tgcaaggcgt cgtcaatggc gtggcggatc tcggcagccg ggatggccgc ctcagcgggc
      481 agcggggccg tcagcagcac gccgcccggc atacgcagcg cgtggcgggc gcgcacgatg
      541 gcggcgacct gcgccgcgtc ttccacgcgg gcgtccactg ataggccgct ctggcgcgtg
      601 tagaaggcgg ggaactcatc ggtctgccag ccgagcaagc tcacggcgtg ggtctccagc
      661 cactcgcggg tcgcgggcag gtcgaggatc gacttggccc cggcgcagac cgtgacgatg
      721 tcgctggcgg ccagcaccgg caagtcggcg gagatgtcca gcgtctgttg ccagccgcgg
      781 tgcaccccgc cgatgccgcc ggtggcgaac accgggatcg ggtgcgggca ggcgcgcgcc
      841 aggtagatgc tcgacgccac cgtcgtcgcg ccgttgcggc gcagggccag agccatgggc
      901 aggtcacgca cgctcagttt ggcgatattg ggatcggtcg ccaggcgctc cagcgcggcg
      961 gcgtcgcaac caatatggac gcgcccgtcg aagacggcga tggttgccgg gacggcgccc
     1021 gcctggcgta cagcgtcttc gcaagcgcgc gccgtctcca ggttggtcgg gcgcggcagc
     1081 ccgtgggcga tgacggtcga ttccagcgcc accaccgcgc gccccgccgc gaaggccgcg
     1141 gccacttcgg gggcgataac aaagggcgta tccgggtcgt tctgtgcaaa agcggtgctc
     1201 atggcgccag tataccgcag ccgggccggt tcgacaatag cgcggcccat cccgtataat
     1261 gaggtgaaaa cggagcgccg gcccccgcgg gccgcgcaca ctctgcaacc gccggcagat
     1321 ctcagccggg gcctgaacgc tacccgcagc ccccactgag aaccggcggc taatagctga
     1381 cagctaaata ctcgtgcaac agccgctcag cgaggagccg gctggaaagg ccagaggagg
     1441 cactgcattg gattttgaac tctccgagga gcaccggctc ttccgcgaaa gcgtgcgcga
     1501 cttcgcggaa aaagaggtgg cgcccggcgc catcgagcgc gaccggacgc atgaattccc
     1561 cgctgatctg ctcaagcgca tggccgaact cggtctcctg ggcatcccct tccctgagaa
     1621 atacggcggc gccggcggcg acaccctgtc ctacgccatc gcggtcgagg agatcagccg
     1681 ggttgacggc tcgctgggca tcatcctcgc cgcccatatc tcgctggggg cctcgccctt
     1741 ctacctgttc ggcaccgagg aacagaaaaa gcgctggctg gtgccgctgg caagcggcga
     1801 gatgctgggc gctttcggcc tgaccgagcc gggcgcgggg tccgacgccg gcggcacgac
     1861 aaccaccgcc gttcgcgagg gcgacgagtg ggtgatcaac gggcgcaagc tctggatcac
     1921 gtcgggccgc gtggccgggg tgatcgtggc gacggccaag accgaccgcc acgcgcaggg
     1981 cagccggggc attagctcgt tcattatccc gcgcggcacg cccggcctca gctacggcaa
     2041 ggacgaggac aagatgggcc tgcgctcgtc gatcacctcg ccgctgttct tcgagaacgt
     2101 gcgtgtgccc gccgggaata tcctgggcaa agagggcgac gggttcaagc agttcatgca
     2161 gatcctggac ggcgggcgca tcagcatcgg cgcgatgggc ctggggctgg gccagggcgc
     2221 cttcgaagcg gcgctgtcct acgccacccg ccgcgaacag ttcggccagc ccatcgccaa
     2281 cttccaggcc atccagttca agctggccga catggcgatg aagctggagg cggcgcggct
     2341 gttggtctac aaggcggcta tgctcaagga ccagaagaag ccgttcaccc aggcggcggc
     2401 gatggcgaag ctctatgcgt ctgagatcgg cgaggagtgc gcctaccagg ccatccagat
     2461 ccacggtggc atgggctacg cggtggagag ccaggtcgag cgcatgtacc gcgatgtgcg
     2521 cctcaccacc atcggcgagg gcacgtcgga gatccagcgc atggtcatcg cccgcaacct
     2581 gctcaaggaa gcggcgcgga ccgcataggt acgacgatgg acctcgatct gaccgaagaa
     2641 caggcccaga tccgccaact ggcgcgcgag ttcgccgagc gcgagcttgc gccccatgcg
     2701 ctggaatggg atgagaccga gcatttccca cgcgaggtct tcgctcagat gggcgcgctg
     2761 ggcttcgccg ggatgctcat ctccgaggag tacagcggcg tgggcgcgga cacgctgtcg
     2821 tacatcctga tgctcgaaga gatcaataag gtgctgcccg cgctaggcac ggtcgtctcc
     2881 gtgcataact cgctggtggg ccacgccatc gcgcgctggg gcaacgccga gcagcggcag
     2941 gagttcctgc cccgcctggc gacgggcgaa tggctgggcg gctacgccct cagcgaggcc
     3001 cacgccggat ccgacccggc cagtatgcgc accctggccg tgcgcgacgg cgacgagtac
     3061 catatcaccg gcaccaagac ctggatcacc agcggccaga gtgccgacgt gttcatcctg
     3121 ttcgccgtga ccgaccgcaa a
//
LOCUS       DASUYC010001121         2281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_426140,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001121 DASUYC010000000
VERSION     DASUYC010001121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426140"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VF276_18295"
     CDS             complement(<1..484)
                     /locus_tag="VF276_18295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608856.1"
                     /translation="MSGMNFMDSLRKGLDRAGFEADKLMRYNRVRAEAGRLNIQAREQ
                     IQALGERALALHKAGTLRDSELEALARDITDLQSRAKQKEDEANAIQAEDWVEPPDPT
                     PAPPPAQSAPPPAPAYTPSYNPTPSPAPSYSPAPSSYMPAPAPSYTPAPPPAPPPAPP
                     S"
     gene            complement(537..1709)
                     /gene="rodA"
                     /locus_tag="VF276_18300"
     CDS             complement(537..1709)
                     /gene="rodA"
                     /locus_tag="VF276_18300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEX6608857.1"
                     /translation="MYRVTASQISIGARSFTNRWATFDFILLLSTLAACAFGVLMVYS
                     ATHGLGPEPLAWSNLAVQHAFKALIGFVLMLMLTQVEYHMLASFVWPLYAGMVGLLAV
                     TTALGHTAGGATRWFKLGDLPVQPSEIAKLVLVLTLAKYLSDNEKRTDRITVYIGSLI
                     IAGIPMALIFSQPDLGTASVCGVIWLVMVVTARLRWLFLAGSVVLGVPAGWGLWSYLM
                     NTMPDKFQYMQDRLLIFLHPESDLYGQGYNILQARISIGAGGLFGKGFLAGTQSQLHF
                     LKVQYADFIFSVIAEEFGLIGCLALIALLYVIVIRYLHIAMNAPDSYGTLIAVGLAAW
                     LGMHIFVNVGMNLSLMPVVGIPLPFISYGGSSMLSLLAAQGLLQSIALRSRKVIFA"
     gene            complement(1795..>2281)
                     /locus_tag="VF276_18305"
     CDS             complement(1795..>2281)
                     /locus_tag="VF276_18305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608858.1"
                     /translation="DRVLDEATPLDTSPARAAVCGPVIAMEGMIREVPPAQLAWCILR
                     GGVFVGPGTFQEGIIAGLRAGTEVVPCDGHNFVSLVHVADMAAAVLAAIRHAPGGSTF
                     NINADSLRQGAYLDELAARRGAAPPPRDPVRPCPPSFRCSNAAAQATLHWAPDHGIWP
                     S"
BASE COUNT          403 a          751 c          779 g          348 t
ORIGIN      
        1 tcgacggcgg cgcgggcggc ggcgcgggcg gcggcgcggg cgtgtagctc ggcgccggtg
       61 cgggcatgta actcgacgga gcggggctgt agctcggcgc gggcgacggc gtcgggttgt
      121 agctcggcgt ataggcgggc gcgggcggcg gcgccgattg cgcgggcggc ggcgcggggg
      181 tcgggtcggg cggttccacc caatcctcgg cctggatcgc gttggcctcg tcttccttct
      241 gcttggcgcg gctctgcaaa tcggtgatgt cgcgggccag ggcctccaac tcgctgtcgc
      301 gcagcgtgcc cgccttgtgc agggccagcg cccgctcgcc gagggcctgg atctgctcgc
      361 gcgcctggat attgagccgc ccggcctcgg cgcgcacccg gttatagcgc atcagcttat
      421 cggcctcgaa cccggcccgg tccagcccct tgcgcaaaga gtccatgaaa ttcatgcccg
      481 acattcccgc aacctccccg aaagatgacg gatggatgcg ccctgcggcg cgcgaatcac
      541 gcgaatatga cttttcgtga gcgcagtgcg atactttgca acaggccctg cgccgccagc
      601 aggctcagca tggacgaccc gccatagctg atgaacggca gcgggatgcc gaccaccggc
      661 atcaggctca gattcatgcc cacattgacg aaaatgtgca tgcccagcca ggccgccagc
      721 cccacggcga tcagcgtgcc gtagctgtcg ggggcgttca tggcaatatg cagatagcgg
      781 atgacgatca cgtagagcag cgcgatcagc gccaggcagc cgatgaggcc gaactcctcg
      841 gcgatgaccg agaagataaa gtcggcgtat tgcaccttga ggaagtgcaa ctggctctgc
      901 gtgccggcca ggaagccctt cccgaacagg ccgcccgctc cgatgctgat ccgcgcctgg
      961 aggatattgt agccctgccc gtacaggtcg ctctccgggt gcaggaagat cagcaaccgg
     1021 tcctgcatgt actggaactt gtcgggcatg gtgttcatca ggtaggacca gaggccccag
     1081 cccgcgggca cgcctagcac caccgagccg gccaggaaga gccagcgcag gcgcgccgtc
     1141 accaccatca ccagccagat cacgccgcag acgctggccg tgcccaggtc gggctggctg
     1201 aagatcagcg ccatggggat cccggcgatg atcaggctgc cgatatagac ggtgatgcgg
     1261 tcggtgcgtt tctcgttgtc gctgagatat ttcgccaggg tcagcaccag cacgagcttg
     1321 gcgatctcgc tgggctgcac cggcaggtcg ccgagcttga accagcgcgt ggcgccgccg
     1381 gccgtgtgcc ccagggccgt ggtcacggcc agcaacccca ccatgcccgc gtagagcggc
     1441 cagacgaagc tcgccagcat gtggtattcc acctgggtca gcatcagcat cagcacgaag
     1501 ccgatcaggg ccttgaaggc gtgctgcacg gccaggttcg accaggccag cggctcgggc
     1561 cccaggccgt gcgtggcgct gtagaccatc aacacgccga aagcgcacgc ggccagggtg
     1621 gacaagagca ggataaagtc gaaggtggcc caccggttgg tgaacgaacg ggcgccgatg
     1681 ctgatttgcg atgcggtaac tcggtacata aggatcctgg gttagcggca gacctttctc
     1741 gcccaatgcg gtaagtcttc acgattatac acgattgcct ccccgatctg ccagtcagga
     1801 cggccagata ccgtgatctg gcgcccagtg taacgtggcc tgcgccgcgg cgttgctgca
     1861 acggaaggag ggcgggcaag ggcgaacggg atcgcgcggc ggcggcgcgg cgccgcggcg
     1921 cgcggccagt tcgtccagat aggcgccctg gcgcagagaa tcggcgttga tgttgaaggt
     1981 cgacccgccc ggcgcgtggc gtatcgccgc cagcaccgcc gccgccatat cggccacatg
     2041 caccagtgag acgaaattgt gaccatcgca gggcaccacc tcggtcccag cccgcaaccc
     2101 ggcgatgatg ccctcctgga aggtgcccgg cccgacaaac accccaccgc gcaagataca
     2161 ccaggcgagc tgcgccggcg gcacctcgcg gatcatgccc tccatggcga tgaccgggcc
     2221 gcaaacggcg gcgcgcgcgg gcgaggtgtc cagcggcgtg gcttcgtcca gcacccggtc
     2281 g
//
LOCUS       DASUYC010001122         5940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_657308,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001122 DASUYC010000000
VERSION     DASUYC010001122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5940
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657308"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1435)
                     /locus_tag="VF276_18310"
     CDS             complement(<1..1435)
                     /locus_tag="VF276_18310"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HEX6608859.1"
                     /translation="MPASKSAPQGAAKTTRANAKTPRNKPAKLSSPDALHAALKQFGH
                     ERFRPGQERIIHDLLRERDVLAVLPTGAGKSLVYQLAAQFLDGVTLVVSPLIALMKDQ
                     VETINEQGVGAGVINSTLGEAEAEDELDKVKQQKAKLLYVTPERFQDQDFLADLAGTP
                     VGLFVVDEAHCVSEWGHSFRPAYLGLAGAAERLGRPTMLALTATATPWIRTEIIDRLG
                     LRDPDLVVREIDRPNLFLEVRRLETEHEVRQTLHDLFLGEAGEYPEALTGQLHEAMQG
                     SGIVYTATTRAAQETADWLNDWGIPADYYHGQRKKADRDRVQDAFMDGELRVIAATNA
                     FGMGVDKPDVRFVVHCDIPGSLEAYYQEAGRAGRDGAFARCTLLYRPADLGQAAFLSG
                     GGHLTPEDIEQARAGLGAQPDGTIEELAEATGLSAGDAARAVTLLRRTGIVEEKAGRV
                     RLLVADFDPATISLEDEESRRAYDRSRL"
     gene            complement(1768..2637)
                     /locus_tag="VF276_18315"
     CDS             complement(1768..2637)
                     /locus_tag="VF276_18315"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6608860.1"
                     /translation="MDESATGAGPGRGFDPVAEDPPDGDPRYPATLTEVQIPSGADRM
                     LGVLLGAAGAGPHPTLLLLHGFPGDERNFDLAHTVRRAGWNVLVFHYRGAWGSPGAFS
                     FAHVLEDVAAALAFLRDPATAATYRADPARLVLVGHSMGGFAALVTAARDPAIPAVGA
                     FTPINLGLLGAEVADLPWLEEVATANFAALMAPLAGTTAADLVREAITQAAAWDLRAL
                     APALAGRTVLLVGAERDTDTPLDLHYTPLAAALAAAPGIALTTHVLAADHVFSERRVA
                     LARLLLAWLDGPA"
     gene            complement(2646..2906)
                     /locus_tag="VF276_18320"
     CDS             complement(2646..2906)
                     /locus_tag="VF276_18320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016514661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608861.1"
                     /translation="MNGLRVQRRCFTVPHVDDNHQAGEAGDNSNEAALSPEQQQRAKN
                     AALIRLLQSWRDSDDADEQRETLAYLMRVLDEDRLSERKLFP"
     gene            complement(2964..4223)
                     /gene="fabF"
                     /locus_tag="VF276_18325"
     CDS             complement(2964..4223)
                     /gene="fabF"
                     /locus_tag="VF276_18325"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX6608862.1"
                     /translation="MGEQKRVVVTGMGAIAPVGNTVEECWANISAGRSGLGPITRFDA
                     SGLETRIAGEVKGFTPEAYIPAKEVRRMDRFIQFAVATAGQAIASANYTVTPENAPDC
                     GVLVGSGIGGILTIDEQLHVLDNKGPRRVNPFTIPMMLTDLAAGRIAMQFGMTGPNFA
                     VMSACASSANAIGEGMEIIKRGDATAMVCGGAEATILPFAVAAFGVMGALSTRNDEPQ
                     QASRPFEKNRQGFVLAEGAAVLLLEERESALKRGAPILAEILGYGSTADASHVTAPME
                     TGEGAARAMQIALRKAGLRPDQIGYLNAHGTGTPLNEKFETIAIKRAFGDAAYHLPIS
                     STKSMTGHMLGAAGGLEAIFAIKALETGILPPTINYEVPDPDCDLDYIPNEARHKAIE
                     YAMTNSMGFGGHNVSLILAGPDHARAA"
     gene            4694..>5940
                     /locus_tag="VF276_18330"
     CDS             4694..>5940
                     /locus_tag="VF276_18330"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX6608863.1"
                     /translation="MPLVPLGCAWMLGVLLGTWWPGATPDRAGIALAAALAGAGALAL
                     VWPHRGARWAVLVLLAGWLGLARSLIALPPAVPDPHSLRALNVPAAEARSVVAPRREV
                     RGRVLADPDPARAGRAAQVRVAVEAVRVDGAWRPATGGLLAVAGPFVAAVQGDLVECA
                     GVVQDPPPVAGFDYRAWLARHGIESYMDGATVRVLAAAADAPETTLARWRREAGLRTA
                     AMLPEPEAGLLRGLLLGQQKAIDPPLWQDFNTTQTSWLIVVSGAQITMLLLLVFNPAR
                     RALYPWPSALLAGVVVVLYAVFVGLGVPVARAAVMGVLYLGALGLGRPITPINLLAVA
                     ALALTALDPLTFADAGFQLSFAAVAGIIIFEPALARRWRRVPVLGQVTAIAVAAQLTT
                     WPVVAAQFAQVSSIGFPAGILAGL"
BASE COUNT          735 a         1970 c         2247 g          988 t
ORIGIN      
        1 ccaggcggct gcggtcgtag gcgcggcggc tttcctcgtc ttccagcgag atggtggccg
       61 ggtcgaagtc agccacgagc aggcggacgc ggcccgcctt ctcctcgacg atgcccgtcc
      121 ggcgcagcag cgtcacggcg cgcgccgcat cgcccgcgct gaggccggtc gcctcggcca
      181 gttcctcgat ggtgccgtcc ggttgcgccc ccaacccggc gcgggcctgc tcgatgtctt
      241 cgggtgtgag gtggccgccg ccggagagga aggcggcctg gcccaggtcg gcggggcggt
      301 agagaagcgt gcagcgggcg aaggcgccgt cgcgcccggc ccgacccgcc tcctggtaat
      361 aggcttccag gctgccgggg atgtcgcagt ggacgacaaa gcgcacatcg ggcttatcca
      421 cgcccatgcc gaaggcgttg gtcgccgcga tgacgcgcaa ctcgccgtcc atgaaggcgt
      481 cctgcacgcg gtcgcggtcg gcctttttgc gctggccgtg gtaatagtcg gcggggatgc
      541 cccagtcgtt gagccagtcc gccgtttcct gtgcggcgcg ggtcgtcgcc gtgtagacga
      601 tgccgctgcc ctgcatcgcc tcgtgcaact gcccggtcaa cgcttcggga tactccccgg
      661 cctcgcccag gaagaggtcg tgcagcgtct ggcgcacctc gtgttcggtc tccagccgcc
      721 gcacttccag gaacaggttg ggccggtcga tttcgcggac caccaggtcg ggatcgcgca
      781 ggcccaggcg gtcgatgatc tcggtgcgga tccagggcgt ggcggtggcg gtcagcgcca
      841 gcatggtcgg gcggcccaaa cgttcggcgg ccccggccag ccccaggtag gccgggcgga
      901 agctgtgccc ccactcggac acgcagtgcg cctcgtcaac cacgaacagc ccgaccggcg
      961 tgcccgccag gtcggccagg aagtcctggt cctggaaccg ttcgggggtc acatagagca
     1021 gcttagcctt ctgctgcttg actttgtcca gttcgtcctc ggcctcggcc tcgcccaggg
     1081 tgctgttgat cacgccggcg cccacgccct gctcgttgat cgtctccacc tggtccttca
     1141 tcagcgcgat caggggcgag acgaccaggg tgacgccgtc gaggaactgc gccgccaact
     1201 ggtagaccag cgacttgccc gcgccggtgg gcagcacggc cagcacatcg cgctcgcgca
     1261 gcaggtcgtg gatgatgcgt tcctggccgg gccggaagcg ctcatggccg aactgtttca
     1321 gggcggcgtg cagggcgtcc ggcgaggaga gtttggccgg cttgttgcgc ggggttttgg
     1381 cgttggccct ggtggttttg gcggcgcctt gcggcgcgga cttggacgcg ggcatccggt
     1441 gatcctttcg cgggtcgtat ggcggcatgg gtggcgcccg accaacctct cccccaaccc
     1501 ctccccgccg cgggctttca agggtaggtt tttttcttat ccgtcatatg gagtggttgc
     1561 gcgaccgggc tggccccgca aagctagccc cgcaccccac caaatagggt ttgacgctac
     1621 tattgaacga cttacacggc caaaaagcaa cttatactgc tacaacggcg tgccgactcc
     1681 gcccgtagtc gcttcccacc gccctgatgt gacggataag aaaataccta cccttgaaag
     1741 cccgctccgg gaaggggggc aggggggtta ggccgggccg tcgagccagg ccagcagcag
     1801 gcgggccagg gccacgcgcc gctcggagaa gacgtgatcg gcggcgagga cgtgggtggt
     1861 cagggcgatg ccgggggcgg cggccagggc ggcggccagc ggggtgtagt gcaggtcgag
     1921 cggcgtatcg gtgtcgcgct cggcgcccac cagcaggacg gtgcgcccgg ccagcgccgg
     1981 ggccagggcg cgcaggtccc acgccgcggc ctgcgtgatc gcctcgcgca ccagatccgc
     2041 cgccgtggtg ccggccagcg gggccatcag cgcggcgaag ttggccgtgg cgacctcctc
     2101 cagccagggc agatcggcca cctcggcgcc cagcaggccc aggttgatcg gcgtgaacgc
     2161 cccaaccgcg ggaatcgccg ggtcgcgggc cgccgtcacc agggcggcga acccgcccat
     2221 gctgtggccc accaggacga ggcgggccgg gtcggcgcga taggtcgccg cggtcgcggg
     2281 gtcgcgcagg aaggcgagcg cggcggccac gtcctccagc acatgggcga agctgaaggc
     2341 gccggggctg ccccaggcgc cccggtagtg gaacacgagc acgttccacc cggcgcggcg
     2401 cacggtgtgg gccaggtcga agttgcgctc gtcgccgggg aagccgtgca gcagcagcag
     2461 cgtggggtgg ggcccggcgc ctgccgcccc cagcagcacc ccgagcatac gatccgcgcc
     2521 gctggggatc tgcacctcgg tgagcgtggc gggatagcgc gggtcgccgt cgggcgggtc
     2581 ctccgccacg gggtcaaacc cgcgccccgg ccccgcgccg gtcgccgatt cgtccatgcc
     2641 ccggctcatg gaaacagttt tcgctcggac agccggtcct cgtcgaggac ccgcatcaga
     2701 taggccaggg tctcgcgttg ctcgtcggca tcgtcgctgt cgcgccagga ttgcagcagg
     2761 cgtatgagtg ccgcgttctt tgctcgttgt tgctgctccg gcgacagggc ggcttcgttg
     2821 ctattgtcgc cggcctcgcc agcctggtgg ttatcatcta cgtgcggcac ggtaaagcac
     2881 cttctttgca cgcgaagacc gttcatatca gtcgtcttct tcgcgtcctt cgcgtcttcg
     2941 cggtgaaata ggcgcggggc cagttacgcg gcgcgggcgt ggtcggggcc ggccaggatg
     3001 agcgagacgt tgtggccgcc gaagcccatc gagttggtca tggcgtattc gatggccttg
     3061 tggcgggctt cgttcgggat gtagtcgagg tcgcagtcgg ggtcgggcac ctcgtagttg
     3121 atggtcggcg gcaggatgcc ggtttcgagc gccttgatgg cgaagatggc ttcgaggccg
     3181 cccgccgcgc ccagcatgtg cccggtcatc gacttggtcg agctaatcgg caggtggtag
     3241 gcggcgtcgc cgaaggcgcg cttgatggcg atggtctcga acttctcgtt gagcggggtg
     3301 ccggtgccgt gggcgttgag gtagccgatc tggtcggggc gcaggccggc cttgcgtagc
     3361 gcgatctgca tggcgcgcgc cgcgccctcg ccggtctcca tcggcgcggt gacgtggctg
     3421 gcgtcggcgg tcgagccgta gcccaggatc tcggccagga tgggcgcgcc gcgtttgagc
     3481 gcgctctcgc gctcttcgag cagcagcacc gccgcgccct cggccaggac gaagccctgg
     3541 cggttcttct cgaacgggcg gctggcctgc tgcggctcgt cgttgcgggt ggacagcgcg
     3601 cccatcacgc cgaacgcggc gacggcgaag ggcaggatcg tggcctcggc gccgccgcag
     3661 accatcgccg tggcgtcgcc gcgcttgatg atctccatgc cctcgccgat ggcgttggcg
     3721 ctcgacgcgc aggccgacat gacggcgaag ttggggccgg tcatgccgaa ctgcatggcg
     3781 atgcgcccgg cggccaggtc ggtcagcatc atcgggatgg tgaacgggtt gacgcggcgc
     3841 ggccccttgt tgtcgagcac gtgcaattgc tcgtcgatgg tgaggatgcc gccgatgccc
     3901 gagccgacga gcacgccgca gtcgggcgcg ttctcgggcg tgaccgtgta gttggcgctc
     3961 gcgatggcct ggccggcggt ggcgacggcg aactggataa agcggtccat gcgccgcact
     4021 tccttggccg ggatataggc ttcgggggtg aagcccttga cctcgccggc gatgcgcgtc
     4081 tccaggcccg acgcgtcgaa ccgcgtgatc ggcccgagac ccgaccgccc ggcgctgata
     4141 ttggcccagc attcctccac tgtgttgccg acgggggcga tggcgcccat gcccgtgacc
     4201 acgactcgct tctgctcgcc caccttgcct actcctttgc gttgggggcg cgcggcccca
     4261 tgttcgtgcg tctctccgta ccgccgagtc taccacaaag cgcgccgggg cggcaaccgg
     4321 ggcgacgagt ggtcagtggt cagtggtcag tggtcagtgg tcagtggtcg gtggtcagtg
     4381 gtcagcggtc agcggtcagt ggtcagtggt cagtggtcag gagacgagtc atgggcgggc
     4441 tagcgcgcgg gtgcggcgcc gcccggcccg tgaacgggcg ggctacggca accaagcccg
     4501 cctgcgcggg ctgggctaca tgaagagagg ccgcgcaggc ggccttcgta cgggtagccg
     4561 gaggcttgag cctccgggct accccgccgc ttgacacccg ccggcccccg tgctatgatc
     4621 cggcttgctt caccgggact atcagatacc gctattgttc gcgctgttta cggggtgctt
     4681 gcgagggtga gtcatgccgc tcgtgccgct gggctgtgcc tggatgctgg gggtgttgct
     4741 ggggacgtgg tggccggggg cgacgcccga tcgcgcgggc atcgcgctgg cagcggcatt
     4801 ggcgggcgcg ggggcgctgg cgctggtgtg gccgcatcgc ggcgcgcgct gggcggtgct
     4861 ggtgctgctg gcgggctggc tggggctggc gcgcagcctg atcgcgctgc ccccggcggt
     4921 gcccgatccg cacagcctgc gtgcgctgaa tgtgcccgcc gccgaggccc gctcggtcgt
     4981 cgcgccgcgg cgcgaggtgc gcgggcgggt gctggccgac cccgacccgg cgcgggcggg
     5041 ccgcgcggcc caggtgcgcg tggcggtcga ggcggtgcgg gtggacggcg cgtggcgccc
     5101 ggccacgggc gggctgctgg cggtggccgg gccgttcgtc gccgccgtgc agggcgacct
     5161 ggtggagtgc gcgggcgtgg tgcaggaccc gcccccggtg gccggcttcg actaccgcgc
     5221 ctggctggcc cgccacggca tcgagtcgta catggacggc gcgacggtgc gcgtgctggc
     5281 ggcggcggcg gacgcgccgg agaccaccct ggcccgctgg cgccgcgagg cggggctgcg
     5341 cacggcggcc atgctgccgg agccggaggc ggggctgttg cgcgggctgt tgctcggcca
     5401 gcagaaggcc atcgacccgc cgctctggca ggacttcaac acgacgcaaa cttcctggct
     5461 gatcgtcgtg tccggcgcgc agattacgat gctcttgctg ctggtcttca acccggcgcg
     5521 gcgggcgctg tacccgtggc cgagcgcgct gctggcgggc gtggtggtgg tgctctacgc
     5581 cgtgttcgtg ggcctgggcg tgccggtggc gcgcgccgcc gtcatgggcg tgctctacct
     5641 gggcgcgctg gggctgggcc ggcccatcac gccgatcaat ctgctggcgg tggccgcgct
     5701 ggcgctgacc gcgctcgacc cgttgacgtt tgcggatgcc gggttccagc tttctttcgc
     5761 ggcggtggcc gggatcatca tatttgagcc ggccctggcc cgccgctggc ggcgggtgcc
     5821 ggtgctgggg caggtcacgg ccattgcggt ggcggcccaa ctgaccacgt ggccggtggt
     5881 cgcggcgcag ttcgcgcagg tctcgtccat cggctttccc gccggcatcc tggccgggct
//
LOCUS       DASUYC010001123         3020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1206215, whole genome shotgun sequence.
ACCESSION   DASUYC010001123 DASUYC010000000
VERSION     DASUYC010001123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1206215"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2389
                     /locus_tag="VF276_18335"
     CDS             <1..2389
                     /locus_tag="VF276_18335"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX6608864.1"
                     /translation="WAWMRKYIPIISSQFRRLGASCDWTRARFTMDPGPARAVRTVFK
                     HLYDKKLIYQGERIINWCPRCMTSLSDLEVENEETPGSLWTIRYPLAEGDGGIIVATT
                     RPETMLGDTAVAVHPDDARWRGLIGKQARLPIMDRLIPIIGDEAVDPAFGTGAVKITP
                     AHDPLDFEIGQRHHLPAINIMNLDSSLNENAGPYQGLKPLDAREQILERLSAEGLLLK
                     TEPHTHMVPHCERCNTVLEPMISRQWFVDIKPLAEPAIAAAQDGRITFVPDRFQKVYM
                     HWMENIRDWNISRQLWWGHRIPVWYCANGHQFASAADPETVTTCEICGSAEIRQDPDV
                     LDTWFSSGLWPFTTLGWPDKTPDYDYFYPTQVMETGYDILFFWVARMIMQGLENTGEA
                     PFSTVYLHGLVRDEIGRKMSKSIGNTVNPLEAADKYGTDALRFNLATGSTPGNDMKFS
                     ESRLEGMQRFANKLWNATRFIVFESPGGTRRVPEAESLRLELPPPERRTLADRWILAR
                     QHDLAADVQRLMEDYQFGEAGRLIYEFIWGEFCDWYLECAKGPLNGDDAQAKADTIAV
                     LRWTLERSLRLLHPFMPFVTEELWQYLLGRPADRAGLPPAEQSIMVRPWPRPEEAPAD
                     PAAERDFGLLMAIITAIRNARAEAIKDAPEGQKADLARRRIAAYVAAGAWAPLLEAQR
                     DILIRLAHLDPANVIIGAEMPEAASAGRMTPLVVEDVAIALPMAGLVDLDAVKARLAT
                     EAAQAEAEIARIVALLANDQFVSKAKPAVVDAQREKLTAARDRLAVLQTRLASFE"
     gene            2648..2893
                     /locus_tag="VF276_18340"
     CDS             2648..2893
                     /locus_tag="VF276_18340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608865.1"
                     /translation="MDTTTRDRKLAFFRRMIWWLPVLAFAIWAAVLVAVGGATAYAGG
                     SFMGVILQALVVGVVVGIVCVAVYFGYKYMLERNPNL"
BASE COUNT          482 a         1035 c         1005 g          498 t
ORIGIN      
        1 ctgggcctgg atgcgcaagt acatcccgat catcagcagc cagttccgcc gcctgggcgc
       61 gtcgtgcgac tggacgcgcg cccgcttcac catggatccc ggcccggcgc gtgccgtgcg
      121 caccgtgttc aagcacctct acgacaagaa gctgatttat caaggcgagc gcatcatcaa
      181 ctggtgcccg cgctgcatga cctcgctgtc cgacctggag gtggagaacg aggagacgcc
      241 cggctcgctc tggaccatcc gctacccgct ggccgaaggc gacggcggca tcatcgtggc
      301 gacgacccgc cccgagacga tgctgggcga caccgccgtg gccgtgcacc cggacgacgc
      361 ccgctggcgc ggcctgatcg gcaagcaggc gcgcctgccg atcatggacc gcctgatccc
      421 gatcatcggc gacgaggcgg tggacccggc cttcggcacc ggcgccgtga agattacgcc
      481 cgcgcatgac ccgctcgact tcgagatcgg tcagcgccac cacctgcccg cgatcaacat
      541 catgaacctg gactcgtcgc tcaacgagaa cgccgggccg taccagggcc tcaagccgct
      601 ggatgcccgc gagcagatcc tggagcgcct gagcgccgag gggctgctgc tcaagaccga
      661 gccgcacacg cacatggtgc cccattgcga gcgctgcaac accgtgctgg agccgatgat
      721 ctcccgccag tggttcgtgg acatcaagcc gctggccgaa cccgccatcg ccgccgccca
      781 ggatgggcgc atcaccttcg tgcccgatcg cttccagaag gtgtacatgc actggatgga
      841 gaatatccgc gactggaaca tcagccgcca gctctggtgg ggccaccgca tcccggtgtg
      901 gtactgcgcc aacggccacc agttcgccag tgccgccgac ccggagaccg tgaccacctg
      961 cgagatctgc ggtagcgccg agattcgcca ggacccggac gtgctcgaca cctggttctc
     1021 gtcgggcctc tggccgttca ccacgctcgg ttggccggac aagacgccgg actatgacta
     1081 cttctacccg acgcaggtga tggagaccgg ctacgacatc ctgttcttct gggtggcgcg
     1141 catgatcatg cagggtctgg agaacaccgg cgaggcgcct ttctccaccg tgtacctgca
     1201 cgggctggtg cgcgacgaga tcgggcgcaa gatgagcaag tccatcggca acacggtgaa
     1261 cccgctggag gccgccgaca agtacggcac cgacgcgctg cgcttcaacc tggcaaccgg
     1321 ctcgacgccc ggcaacgaca tgaagttctc ggagtcgcgc ctggaaggga tgcagcgctt
     1381 cgccaacaag ctctggaacg cgacgcgctt catcgtgttc gagagtcccg gcggcacgcg
     1441 gcgcgtgccg gaggctgaaa gcctgcgcct ggagttgccg ccgcccgaac gccgcacgct
     1501 ggccgaccgc tggatcctgg cccggcagca tgacctggcc gccgacgtgc agcggttgat
     1561 ggaggactat cagttcggcg aggccggccg cctgatctac gagttcatct ggggcgagtt
     1621 ctgcgattgg tacctggaat gcgccaaggg gccgttgaac ggtgacgacg cgcaggccaa
     1681 agccgacacc atcgccgtgt tgcgctggac gctggagcgg tcgctgcgcc tgctgcaccc
     1741 gttcatgccc ttcgtgaccg aggagctgtg gcagtacctg ctgggccggc ccgccgaccg
     1801 cgccggcctg ccgcccgccg agcagagcat catggtgcgc ccgtggccgc gcccggagga
     1861 agcgcccgcc gaccccgccg ccgagcgcga cttcggcctg ctgatggcga tcatcacggc
     1921 gatccgcaac gcgcgggccg aggcgatcaa ggacgcgccg gagggccaga aggccgacct
     1981 ggcgcggcgg cgcatcgccg cctacgtggc ggcgggcgcc tgggcgccgc tgctggaggc
     2041 ccagcgcgac atcctgatcc ggctggcgca cctcgacccg gccaacgtga tcatcggcgc
     2101 ggagatgccg gaggcggcct ccgccgggcg gatgaccccg ctggtggtgg aggacgtggc
     2161 gattgccctg cccatggcgg gcctggtgga cctcgacgcc gtaaaggcgc gcctggcgac
     2221 cgaggccgcg caggccgagg ccgagatcgc tcgcatcgtg gcgctgctgg cgaacgacca
     2281 gttcgtgagc aaggccaagc ccgccgtggt ggacgcccag cgcgaaaagc tcacggcggc
     2341 ccgcgaccgc ctggccgtgt tgcagacccg gctggcgtcg ttcgagtaag cgcgcagata
     2401 ccggggccgg ggggaagtcg tgtctgcttc cccccggcct tttgtgcttg ctaccctatt
     2461 agccccggtc tcgcggtgat tttcggcccg gtagtccccc cttgcctagc ctattcgggc
     2521 tattgacttg gtttgttcag gtgtgctacg atgcccttat catcaagctt accgggcttg
     2581 ctgagtggca tggggccgta actcatcgtc gcattccgtc acactactcg gtaaaggagg
     2641 tgaatacatg gacacaacta ctcgcgaccg caaactcgcg tttttccgcc gcatgatctg
     2701 gtggttgccc gtgctggcct tcgccatctg ggccgccgtg ctcgtcgccg tcggtggcgc
     2761 gactgcctac gccgggggca gcttcatggg tgtcatcttg caggccctgg tcgtcggcgt
     2821 cgtagtcggc atcgtctgcg tggccgtcta cttcggctat aagtacatgc tggagcgcaa
     2881 cccgaatctc taacccgctg tcctggggac atggccttgc cgcgtttcgt ggtcgcaggc
     2941 aggcggcgtc cggctcaggc cggcgcatta tgcgtcgcgc aggatacccg gcgtagcgct
     3001 ttccggtgat tgcccgcggc
//
LOCUS       DASUYC010001124         6175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_195094,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001124 DASUYC010000000
VERSION     DASUYC010001124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6175
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195094"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="VF276_18345"
     CDS             complement(<1..508)
                     /locus_tag="VF276_18345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX6608866.1"
                     /translation="MYVLGTAGHVDHGKSTLVKALTGIDPDRLQEEKEREMTIDLGFA
                     WLRLPGGPEVSIVDVPGHERFIKNMLAGVGGIDAALLVIAADEGPMPQTAEHLNILHL
                     LGVERGLVVLTKRDLVDSDWLDLVQEEVRDRLAGTTLAGAPLIAVSARTGEGLPELKT
                     AIATLLAGT"
     gene            complement(512..1816)
                     /locus_tag="VF276_18350"
     CDS             complement(512..1816)
                     /locus_tag="VF276_18350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608867.1"
                     /translation="MPTLFRKKPAPVAAGASKSMPRRVGTPSAPVGPTYVVPGYPGAA
                     ASAAAEKGQRFRYPTRFWLPLRLVGYALILLSVLNLVNTMGRFVQLLSNFSVYSVLLL
                     ITAFASLLFSVVAGALIADALPSLEVRSDGLAVTEFVRWRTLPWQRIVKLHSMALPGD
                     RYVVFVQYAGAPLSPEHIVYSLLAGLGAKSGFFYTSDIKDFDDLTRSILDARMRAVPG
                     TRVEDLLVEDVPMPVVQMAFNPMGTLTKVAGFTEGEVSSPLMNLEERPTIPRSQLIRM
                     QLGITLVPVILFLIDNLINGGIPILTSSTGSLVLGTFGLLALGLLELPFVALAIQAVG
                     ETVLGHGEFQVALNTYPYLELPRMLGFVVIFALIAIKAPFILVFLVWLGALGWTTYLT
                     LLFTQRLYRITQNQAIMAGGAAAIVQLFVLIAYMMLRAPGLA"
     gene            2241..4289
                     /locus_tag="VF276_18355"
     CDS             2241..4289
                     /locus_tag="VF276_18355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair helicase XPB"
                     /protein_id="HEX6608868.1"
                     /translation="MPYNPANPLIVQSDRSILLEVDNPLYEAARDDLARFAELEKSPE
                     HIHTYRVTPLSLWNAAASGSTAAEIVAALTRYSKFDLPGNVLADIEDYVARFGRVKLL
                     VDESGRLVLRSTDTLLITEIQRNSHTRLFLRGRIDDQTLEVDPAARGHIKQALVEFGY
                     PAEDLAGYVDGEELRFELRPVTNGGEPFDLRHYQRDSVGAFWAGGSSQGGSGVIVLPC
                     GAGKTVVGMGAMEKVQASTLILAPSTSAVRQWIRELLDKTTLTPDQVGEYTGEHKEIR
                     PVTVTTYQIMTYRPFTVNENTGELGEFPHFQLFTNHNWGLIIYDEVHLLPAPVFRVTA
                     EIQARRRLGLTATLVREDGREEDVFSLIGPKKFDVPWKDLEQQGWIATAECAEIRIKL
                     LPSERLDYVMAEDSRIKYRIAAENPLKLDVLMALIEQHRDDNVLVIGQYIEQLKQIAA
                     QLQAPLITGKTPNHERETLYRDFREGRIKLLVVSKVANFAIDLPDANVAIQVSGTFGS
                     RQEEAQRLGRILRPKADGGPAHFYTLVTRDTKDQDFSANRQLFLTEQGYRYTIMDAHD
                     IVPDVEAKVAAEQRLLEQEGADALRARAQERRKAMLNGSEDGDDEDGAPRNGRKATAT
                     APAPADGTKKRRGRPPKVRPETVQASDAPAPATNGNGAHPAGNGTGAERSRGSLRLVK
                     "
     gene            4383..5753
                     /locus_tag="VF276_18360"
     CDS             4383..5753
                     /locus_tag="VF276_18360"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX6608869.1"
                     /translation="MSVIAVLGCQWGDEGKGKIVDLLASRARVVVRCNGGPNAGHTIV
                     NSMGTFKMRLTPSGIFHPRTICLIGNGVVVDPGILLEEIDTLQAAGVDISRLLISDRA
                     HVIMPYHRILDELEEKDRAASGDGFAIGTTKRGIGPAYADKTARIGIRMADLQHEETL
                     LSKLSVVLNYKNRILTKLYGVPPVSLHDTYLQLVEYGRRLGKHIISSDLVLNRALGEK
                     HHILLEGAQGALLDLDIGTYPYVTSSNPTTAGLCVGAGLPPTAVDGALGVYKAYQTRV
                     GEGPFPTELTGEQADFLRSQGGAGHEEFGTVTGRPRRVGWFDAVAGRYIARANGLTAL
                     AITRLDSLDRFPVIKICTGYQVNDMLLRSVPAEIATMEIVQPQYEEHPGWMAPTSDCR
                     QWDDLPANARKYLSRLRELMGVRIDLVSVGPDREQTILLREPFDALPDIVSRFVDTVQ
                     DRGATL"
     gene            5769..>6175
                     /locus_tag="VF276_18365"
     CDS             5769..>6175
                     /locus_tag="VF276_18365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608870.1"
                     /translation="MSAAVPADVLTLAGGLLRLGTLPAGLLLGGGAGAGLLLRHLGRD
                     PAAVEQAGRRLARPLLLILIAALVPLAVVETLQQTGAPLNLVLLTDLLPYMLLNTLFG
                     VVWAAHAVILGALWARLRAGHAPGLVGALALLLA"
BASE COUNT         1110 a         2095 c         2048 g          922 t
ORIGIN      
        1 tggtcccggc cagcagcgtg gcgatggccg tcttgagctc cggcagcccc tcgcctgtgc
       61 gcgccgacac ggcgatcagc ggcgccccgg ccagcgtcgt gcccgccagc cggtcgcgga
      121 cttcttcctg caccaggtcg agccagtcgc tatccaccag gtcgcgcttg gtcagcacga
      181 ccagcccgcg ctcgacgccc agcaggtgga ggatgttcag atgctccgcc gtctggggca
      241 tcggcccctc gtcggcggcg atcaccagca gcgcggcgtc gatgccgccc acgcccgcca
      301 gcatgttctt gatgaagcgc tcgtggcccg gcacatcgac aatcgaaacc tcgggtccgc
      361 ccggcaagcg aagccaggcg aagccgaggt cgatggtcat ttcccgttct ttttcttctt
      421 gcaagcgatc cggatcgatc ccggtcagcg ccttaaccaa cgtggatttg ccgtggtcga
      481 cgtggccagc ggtgcccagt acatacatgc cctaggccag tccgggcgcg cgcagcatca
      541 tataggcgat cagcacgaac agttgcacga tcgccgcggc cccgcccgcc atgatcgcct
      601 ggttctgggt gatgcgatac aggcgctggg tgaacagcag ggtcaggtag gtcgtccagc
      661 ccagcgcgcc gagccagacc aggaacacca ggatgaacgg cgccttgatc gcgatgaggg
      721 cgaagatgac gacgaagccg agcatgcgcg gcagttcgag gtagggatag gtgttcaggg
      781 ccacctggaa ctcgccgtgc cccagcacgg tctcgcccac ggcctggatc gccagggcga
      841 cgaagggcaa ttccagcaac ccgagcgcca gcaggccgaa ggtgcccagc accaggctgc
      901 cggtgctgct ggtcaggatc gggatgccgc cgttgatcag gttgtcgatc aggaacagga
      961 tgaccggcac cagcgtgatg ccgagctgca tgcggatcag ttgcgaccgc gggatcgtgg
     1021 gccgctcttc gaggttcatc agcggcgagc taacctcgcc ctcggtgaaa ccggccacct
     1081 tggtcagcgt gcccatgggg ttgaaggcca tctgcacgac cggcatcggc acgtcttcga
     1141 ccagcagatc ttcgacgcgc gtgccgggga cggcgcgcat acgggcatcc aggatgctgc
     1201 gggtcaggtc gtcgaagtcc ttgatgtccg aggtatagaa aaacccggac ttggcaccca
     1261 gcccggccag caacgaatag acgatgtgct cgggcgagag cggcgccccg gcatattgca
     1321 cgaagacgac gtagcggtca cccggcaggg ccatcgagtg cagcttgacg atgcgttgcc
     1381 agggcagcgt ccgccagcgc acgaactcgg tcacggccag accgtccgag cgcacttcca
     1441 ggctgggcag cgcgtcggcg atcagcgcac cggcgaccac gctgaacagc aaactggcga
     1501 aagccgtgat cagcagcaag acgctgtaga cgctgaaatt ggacagcaac tggacgaagc
     1561 gccccatcgt gttgaccagg ttgagcacgc tgagcaaaat cagcgcatag cccaccagcc
     1621 gcaacggcag ccagaagcgg gtcgggtagc ggaaccgctg gcccttctcg gcggcggcag
     1681 aggcagcggc gcccggataa ccaggcacca catacgtcgg ccccacgggg gcgctaggag
     1741 tacccacgcg gcgcggcatg gacttagaag cacccgccgc caccggcgcg ggcttcttgc
     1801 ggaataatgt aggcacgggg atccctcctt tgtcggaccg ggcggactcc ggtcgtcctc
     1861 aatcccttca gtttatcatg agcttaccgc atttggcaat agcccatttg ggtcagatgc
     1921 gcctgggagg ggctggccgg cccgtcgtgc ggcgagagat acgacgccgc attacacccg
     1981 cgaaaacacc ttcacgaagc cttcagcacg agttccatcc gccggctatg tcgcccgccc
     2041 aagccgccac cggccaggag gatcgccccg atgtggaagg ggcctgccaa atgtggtaca
     2101 atacgatgct gccctttcgg gcgcgcgccg gatagcttgc gatgatgcga tctgccgcgg
     2161 tgcggatcgc ataatgcgcc tggcccatgt aacggcgcac cgatccggcg cgaatgttca
     2221 aggaaaacga ggtctcccgc atgccctaca acccggccaa tccgctcatc gtgcaaagtg
     2281 atcgctcgat cttgctggaa gtcgataacc cgctctacga ggcggcccgc gacgacctgg
     2341 cgcgcttcgc cgaactggag aaaagccccg aacacatcca tacctaccgc gtcacaccgc
     2401 tgtcgctgtg gaacgccgcg gcttcggggt caaccgccgc cgaaattgtc gccgcgctga
     2461 cccgctacag caagttcgac ctgcccggca acgtcctcgc cgacatcgaa gactacgtcg
     2521 cccgctttgg ccgggtcaag ttgctggtgg acgaatcggg gcggctggtg ctgcgctcga
     2581 cggacacgct gctcattacc gagatccagc gcaattcgca cacgcggctc ttcctgcgcg
     2641 ggcggatcga cgaccagacg ctcgaagtcg acccggcggc gcgcgggcat atcaagcagg
     2701 cgctggtcga gttcggctat ccggcggagg acctggcggg ctatgttgac ggcgaggaac
     2761 tccgcttcga gttgcgcccc gtgaccaacg gcggcgaacc gttcgatctg cgccactacc
     2821 agcgcgactc ggtgggcgcc ttctgggcgg gcggcagcag ccagggcggc agcggggtga
     2881 tcgtgctgcc ctgcggcgcg ggcaagacgg tggtcggcat gggcgccatg gaaaaggtcc
     2941 aggcgagcac gctcatcctg gcccccagca cgtcggcggt gcgccagtgg atccgcgagc
     3001 tgctcgataa gacgacgttg acgccggacc aggtgggcga gtacacgggc gagcacaaag
     3061 agatccgccc ggtgacagtc accacctacc agatcatgac ctaccggccc ttcacggtga
     3121 acgagaatac gggcgaactg ggcgagttcc cgcacttcca actgttcacc aaccacaact
     3181 ggggcctgat catctacgac gaggtccacc tgctgcccgc accggtgttc cgcgtgaccg
     3241 ccgagatcca ggcccgccgc cgccttggcc tcaccgccac gctggtgcgc gaggacgggc
     3301 gcgaggaaga tgtattctcg ctgatcgggc cgaagaagtt cgacgtgccc tggaaagacc
     3361 tggaacagca gggctggatc gccaccgccg agtgcgcgga gatccgcatc aagctgttgc
     3421 ccagcgagcg cctggactac gtcatggccg aggactcgcg catcaagtac cgcatcgccg
     3481 ccgagaatcc gctcaagctg gacgtgctga tggccctgat cgagcagcac cgcgacgaca
     3541 acgtgctggt catcgggcag tatatcgagc agttgaagca gatcgcggcc cagttgcagg
     3601 ccccgctgat caccggtaag acgccgaatc acgagcgcga aacgctctac cgcgatttcc
     3661 gcgagggccg catcaagctg ctggtggtct ccaaggtggc gaatttcgcc atcgacctgc
     3721 ccgacgccaa cgtggcgatc caggtgtcgg ggacgttcgg ctcgcgccag gaagaagcgc
     3781 agcggctggg ccgcatcctg cgccccaagg ccgacggcgg ccccgcgcac ttctacacgc
     3841 tggtgacgcg cgacaccaag gaccaggact tttcggccaa ccgccagctc ttcttgaccg
     3901 aacagggcta ccgctataca atcatggacg cgcacgatat tgtgcccgac gtggaggcta
     3961 aggtggccgc ggaacagcgc ctgctggagc aggagggcgc cgatgcgctg cgcgcgcggg
     4021 cacaagagcg gcgcaaggcg atgctgaacg gcagcgagga cggggacgat gaggacggcg
     4081 cgccgcgcaa cgggcgcaag gcgacggcca ccgccccggc gcccgccgac ggcacgaaga
     4141 agcgccgggg ccgcccgccg aaggtccggc ccgagaccgt ccaggcgagc gatgcacccg
     4201 cgcccgccac caacggcaac ggcgcgcacc ccgcgggcaa cggcaccggg gccgagcgga
     4261 gccggggcag cctgcgctta gtcaagtagc ggcagccggc ggacgctgta agggccggtt
     4321 tcaaaccggc gcgctgcggc aacacggtag ggactgctag cccaatccag aggaggaaag
     4381 ctgtgtcggt aatcgcggtt ttgggatgcc agtggggcga tgagggcaag ggcaagatcg
     4441 tggacttgct cgccagccgg gcgcgcgtcg tggtccgttg caacggcggc cccaacgccg
     4501 ggcacaccat cgtcaactcg atgggcacct tcaaaatgcg cctcaccccg tcggggatct
     4561 tccacccgcg caccatctgc ctgatcggca atggggtcgt cgtggacccc ggcatcctgc
     4621 tggaggagat cgacaccctg caagccgccg gggtcgacat cagccgcctg ctgatcagcg
     4681 accgggcgca cgtgatcatg ccctaccatc gcatcctcga tgaactggag gagaaggacc
     4741 gcgcggcgtc cggcgacggc tttgccatcg gcacgaccaa gcgcggcatc ggtccggcct
     4801 acgccgacaa gacggcgcgc atcggcatcc gcatggccga tctgcaacac gaagagacgc
     4861 tgctcagcaa gctctcggtc gtgctgaact acaagaaccg catcctgacc aagctctatg
     4921 gcgtgccgcc ggtcagcctg cacgacacct acctgcaact ggtggagtat ggccgccgcc
     4981 tgggcaagca cattatttcc agcgatctgg tgctcaaccg cgccctcggc gagaagcatc
     5041 acatcttgct cgaaggcgcg cagggcgccc tgctcgacct cgacatcggc acctacccct
     5101 atgtcacgtc cagcaacccg accaccgccg ggctgtgcgt gggcgcgggt ctaccgccca
     5161 ccgccgtgga cggcgccctc ggcgtctata aggcgtacca gacccgcgtc ggcgaaggcc
     5221 ccttcccgac cgaactcacc ggcgagcagg ccgacttcct gcgcagccag ggcggcgcgg
     5281 gccacgagga gttcggcact gtcaccgggc ggccccgccg ggtgggctgg ttcgacgccg
     5341 tggccgggcg ctacatcgcg cgggccaacg gcctgaccgc gctggcgatc acccggctcg
     5401 actcgctcga ccgcttcccg gtgatcaaga tctgcaccgg ctaccaggtg aacgacatgc
     5461 tgctgcgcag cgtgcccgcc gagatcgcca cgatggagat cgtgcagccg cagtatgagg
     5521 agcatccggg ctggatggcg ccgaccagcg attgccgcca gtgggacgac ctgccggcca
     5581 acgcgcgcaa gtatctgtcc cgcctgcgcg agttgatggg ggtgcggatc gacctcgtct
     5641 ccgtcggccc cgaccgtgag cagacgatcc tcctgcgcga acccttcgac gccctgcccg
     5701 acatcgtcag ccgcttcgtc gacacggtgc aagaccgcgg cgcgacgctc tagcacggca
     5761 gccgcggatt gagcgccgcc gtccccgctg atgtgctgac cctggcgggc gggctgttgc
     5821 ggctaggcac cctgcccgcc ggactgctgc tgggcggcgg cgccggagcc gggctgctgc
     5881 tgcggcacct gggtcgcgac cccgccgccg tcgagcaggc cgggcgccgg ctggcccgcc
     5941 cgctgctgct gatcctgatc gccgcgctgg tgccgctggc cgtggtcgag acgctgcaac
     6001 agaccggggc gccgttgaat ttggttttgc tgaccgatct gctgccgtac atgctgctga
     6061 acacgttgtt cggcgtggtc tgggcggcac acgcggtgat cctgggcgcg ctctgggcgc
     6121 gcctccgcgc cgggcacgcg cccggcctgg tgggcgcgct ggcgctactg ttggc
//
LOCUS       DASUYC010001125         3653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1812753, whole genome shotgun sequence.
ACCESSION   DASUYC010001125 DASUYC010000000
VERSION     DASUYC010001125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1812753"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..303
                     /locus_tag="VF276_18370"
     CDS             76..303
                     /locus_tag="VF276_18370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608871.1"
                     /translation="MRAALAAATFTGPRGPVTMDGQTHCTSGPQYLREVQARDGALAN
                     VPVAPLPGGAEAGAALRATTKSGWTNAYLSV"
     gene            366..887
                     /locus_tag="VF276_18375"
     CDS             366..887
                     /locus_tag="VF276_18375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015843035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEX6608872.1"
                     /translation="MPQDPYIGDIYVFAGTYAPRGWAFCEGQILSIAQNQALFSILGT
                     TYGGNGQTTFALPDLRGRVPMHFGQGPGLSPRTLGEKSGTESVTLLTTELPAHIHSVT
                     ASSDNGIRTGPANGVWASSARGDSQYSATADTSMNPAAMGVAGSSQPHNNMQPYLAIR
                     FIIALVGVYPSRT"
     gene            936..1454
                     /locus_tag="VF276_18380"
     CDS             936..1454
                     /locus_tag="VF276_18380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEX6608873.1"
                     /translation="MSEPFLGEIKMFAGNFAPRGWALCNGQILAISQNTALFSLLGTT
                     YGGNGQTTFALPNLQSRIPIAPGQGPGLTNYDLGEEIGTESVTLISTEMPMHSHVPQA
                     QSGPGTSTSPQNNVWAGSSNRDAQYGDTLNTPMNAAALPPAGGGQPHNNLQPYLVINF
                     IIALEGIYPPRS"
     gene            1525..2043
                     /locus_tag="VF276_18385"
     CDS             1525..2043
                     /locus_tag="VF276_18385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEX6608874.1"
                     /translation="MADPYLGEIRMFAGNFAPLGWAFCNGQLLPIAQYDALYAILGTT
                     YGGDGQTTFALPDLRGRGPIHAGQGPGLSNHPQGEQGGTETVTLTVAQMPGHTHVPAA
                     SSAAGTSSHPTGAVWAASSTSDNQYASSTKTPMNPGTVSAAGGNQPHDNLQPLLAINF
                     IIALEGIFPSPN"
     gene            2114..2590
                     /locus_tag="VF276_18390"
     CDS             2114..2590
                     /locus_tag="VF276_18390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006507563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6608875.1"
                     /translation="MRVSLRPVQADDAAFLYELYAATRAAELAQWGWDAAQQDAFLRL
                     QFRAHEQHYARALPHADQQIVLRDAASVGRLIVARNDRDIVLADIALLPAARNAGLGT
                     HLVTAVLAEAAVAGRPVRLHVAVGNPALRLYQRLGFVILAENGTHYFMEKPAPAPG"
     gene            2619..>3653
                     /locus_tag="VF276_18395"
     CDS             2619..>3653
                     /locus_tag="VF276_18395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX6608876.1"
                     /translation="MTVFPHPPARSVIRGVPRAALHVLLVLGLLMAALGYGAASAAPS
                     ATITVTTTADTLDAAAGNCAAITIAGLPGPDGVTSLREAVCAANNSPGSTITFGVNGT
                     FTLTRTGVNEDAAATGDLDLLSNVTITGKGATNTIIDGNNSDRIFDIDPLQGSNAVVS
                     ISGVTIQHGSVNPAAINLGGGVATGGSSTVTISNSTITANQSVSGTGGGLEAFGALTL
                     TNVTVSNNIADAQGGGIRATGNTTISGSTITGNSSEVGGGLWLGSASGISMSVTESTI
                     SGNHAVDRPGGLGAASEDGGGLYIDTDGSVTIQRSTISGNDASRNGGGIYFRDNPSGS
                     VGSLGLTNNTI"
BASE COUNT          630 a         1381 c         1103 g          539 t
ORIGIN      
        1 ctcggctaag agaccgcgcg actggcggcc ctggcgctgg ccgcgaccgg cggcgacccc
       61 acccgcagca cccagatgcg cgccgcgctg gccgccgcga cctttaccgg cccgcgcggc
      121 cccgtcacca tggatgggca aacccattgc accagcggcc cgcagtacct gcgcgaggtg
      181 caggcgcgcg acggcgcgct cgccaacgtg cccgtcgccc cgctgcccgg cggcgccgag
      241 gccggcgccg ccttgcgcgc gaccaccaag tctggatgga ccaacgctta tttatcggtc
      301 tagttccccc ggtggcgcgc ggcaccccgc gcccggcaga ttatcggcta caggaggatt
      361 gagctatgcc gcaggaccct tatattggcg atatatacgt ctttgccgga acctatgccc
      421 cgcgcggctg ggccttctgc gaggggcaaa tcttgtccat cgctcagaac caggccctct
      481 tctccattct cggcacgacc tacggcggca acgggcagac caccttcgcc ctgcccgacc
      541 tgcgcgggcg cgtgcccatg cacttcggcc agggaccggg cctgtccccg cgcacgctgg
      601 gtgaaaagag cggcaccgaa agcgtgaccc tgctaaccac ggaactaccc gcccatatcc
      661 acagcgtcac cgcctcgtcc gataacggca ttcgcaccgg ccccgccaac ggcgtgtggg
      721 ccagttcggc gcgcggcgac tcccagtaca gcgccactgc cgacacgagc atgaacccgg
      781 cggccatggg cgtcgccggc agcagccagc cgcacaataa catgcaaccc tacctcgcga
      841 tccgttttat catcgcgctg gtgggtgtgt atccctcgcg cacctgagcg gccccatgca
      901 cgcggccagc gccgcctctg aaggagcaac ctgcgatgtc cgaaccgttt cttggcgaaa
      961 tcaaaatgtt tgccggcaac ttcgccccgc gcggctgggc gctctgtaac gggcagatcc
     1021 tggccatcag ccagaacacc gccctgtttt ccctgctcgg caccacctat ggcggcaacg
     1081 gccagaccac cttcgccctg cccaatttgc aatcccgcat cccgatcgcg ccgggccagg
     1141 gccccggcct gacgaactac gacctgggcg aggagatcgg caccgaaagc gtgaccctga
     1201 tcagcaccga gatgccgatg catagtcatg tgccgcaggc gcagagcggc cccggcacca
     1261 gcacctcccc gcagaataat gtctgggctg gatccagcaa ccgcgacgcg caatacggcg
     1321 acaccctcaa cacacccatg aacgcagcgg cgctgccccc ggccggcggc gggcagcccc
     1381 ataataacct gcagccctat ctggtgatta atttcattat cgccctggag ggcatctacc
     1441 caccgcgctc ctgaagccgc gcctgccggg gccgtccgcg agtggcgccc ggtcccggcg
     1501 cgttaatgag agaggataga agcgatggct gatccatacc tcggcgagat tcggatgttc
     1561 gccggtaact ttgcccctct gggatgggcc ttctgtaatg gacaacttct gcccattgcc
     1621 caatatgacg cgttgtacgc cattctcggt acgacttatg ggggcgacgg ccagacgacc
     1681 ttcgccctgc ccgacctgcg cgggcgcggc ccgatccacg ccggccaggg gccggggctg
     1741 agcaatcacc cgcagggcga gcagggcggc accgaaacag tcaccttgac ggtcgcccag
     1801 atgcctggcc acacccacgt acccgccgcg tcgtcggcgg ccggcaccag ttcgcacccc
     1861 accggcgcgg tgtgggccgc atccagcact tcagacaacc agtatgcgag ctcgaccaag
     1921 acccccatga atccggggac ggtgagcgcg gcaggcggca accagccgca cgacaacctc
     1981 cagccgctgc tggcgatcaa cttcatcatc gccctggagg gcattttccc gtcgcccaac
     2041 tgaccctggg accgccgctc ccagcacccg gcgcgcccgg tcggaccggg cgcgccccta
     2101 gattcgagtg cttatgcgtg tgagcctccg cccggtccag gcggacgacg cggcgttttt
     2161 gtacgagttg tacgcggcca cccgcgccgc cgaactggcg cagtgggggt gggacgccgc
     2221 gcagcaagac gcctttctgc gcctgcagtt tcgcgcccat gaacagcact acgcccgcgc
     2281 cctgccccac gccgaccagc agatcgtcct gcgcgacgcc gcgtcggtgg gccggctgat
     2341 cgtcgcgcgc aacgaccgcg atattgtgct ggccgatatt gccctgctgc ccgccgcgcg
     2401 caacgccggc ctgggcaccc acctcgtcac cgccgtgctg gccgaggcgg cggtggcggg
     2461 tcgccccgtg cgcctgcatg tcgcggtggg taacccggcg ctccgcctgt accagcgcct
     2521 gggcttcgtc attcttgccg agaacggcac ccattacttt atggagaagc cggcgcccgc
     2581 gcccggctga tccctcactt caggaggagc gaacacccgt gaccgtattt ccccatccac
     2641 ccgcacggtc ggtgatccgg ggcgtgccgc gcgctgcctt gcacgtgttg ctcgtgctgg
     2701 gcctgctgat ggcggccctc gggtacggcg cggccagcgc cgcgcccagc gccacgatca
     2761 ccgtcaccac caccgccgat accctggacg ctgccgccgg caactgcgcg gccattacta
     2821 tcgccggctt gcccggcccc gacggcgtca ccagcctgcg cgaagccgtc tgcgccgcca
     2881 acaacagccc tggctcgacc atcacctttg gcgtcaacgg caccttcacc ctcacccgca
     2941 ccggcgtcaa cgaagatgcc gccgccaccg gcgacctcga cctcctctcc aacgtcacca
     3001 tcaccggcaa gggcgccacc aacaccatca tcgacggcaa caacagcgac cgcatcttcg
     3061 acatcgatcc gctccaggga tcgaacgccg tggtcagcat ctccggcgtg accatccagc
     3121 atggcagcgt caaccccgcc gctatcaacc tgggcggcgg cgtggcgacc ggtggctcct
     3181 cgaccgtgac catcagcaac agcaccatca ccgccaacca gtcggtgagc ggcaccggcg
     3241 gcggcctcga agccttcggc gccctgacgc tcaccaacgt caccgtgtcc aacaacatcg
     3301 ccgacgccca gggcgggggc atccgcgcca ccggcaatac gaccataagc ggcagcacga
     3361 tcaccggcaa ctcgtcggaa gtggggggcg ggctgtggct ggggtcagcg agcggcatca
     3421 gcatgagcgt gaccgagagt accatctccg gcaaccacgc cgtggaccgc cccggcgggc
     3481 tgggggccgc cagtgaggat ggcggcgggc tctacatcga caccgacggt agtgtgacca
     3541 tccagcgcag caccatctcc ggcaacgacg cttcgcgcaa cggcggcggg atctatttcc
     3601 gcgataaccc gagcgggagc gttggcagcc tcggtctgac caacaacacg atc
//
LOCUS       DASUYC010001126         2218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1582867, whole genome shotgun sequence.
ACCESSION   DASUYC010001126 DASUYC010000000
VERSION     DASUYC010001126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1582867"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1498
                     /locus_tag="VF276_18400"
     CDS             <1..1498
                     /locus_tag="VF276_18400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608877.1"
                     /translation="PLVAPQPVSGLGGFLSAIVNRVLGTTPAPPAPVAGPAPLDMPWA
                     RRPAAPAVEEDSAGEYEPAPAPLSVQRAVAAPPAPPAGATAVAALPPTAAAPVADSGP
                     AVAMPAVPTSGDPFAVPVPGDLPAVPAAPAWVSTPAPAFDQTAGELAEAPVAQPWMDL
                     DSLGDVLARVQTPLVPTAGLSTAGQGGLWRRIVGAFERALPGGGAPAAPMTLAWSGGS
                     PATTGAGAAPFVPAQPGGYSAPSAGSAAPWPVYGPGAGAWIPAAGPGGAALLAGRLPA
                     DFGAALGPHALTPAVPVPYFDAESAGDADAEVGDAAQAWIAALDRMYGAPAAGSETMP
                     LAVPYGGFVVPAAGASGVLPQDLAWVAETIGLESAPPSATPARRVWERPVAGAPAPPS
                     WAGGQGPGFVPPAPGWIGDTGSAEYDSEAAAWADVVAAAVESDYGAGVPALALAGEEQ
                     GVPAVQDAPSGADHEAAGDAAGDLDELANSVYEIIRRRLEVERERDWA"
     gene            1522..2190
                     /locus_tag="VF276_18405"
     CDS             1522..2190
                     /locus_tag="VF276_18405"
                     /inference="COORDINATES: protein motif:HMM:NF039279.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX6608878.1"
                     /translation="MPANNGLQEAVIKNLLTGEEVRCLFRPKEYTFAKQNTWTPNRAI
                     GKTMQPPKYTGGQPMTLQMELLFDTYEERGNRDVRRRTDGLWKMMKVADQKKDPQTKK
                     SEPPHVEFRWGRTWTFKAVITSLQQKFTLFDPDGTPVRSTVTISFMQAEEQGQYPGQN
                     PTSGAREGYAVHVVKEGETIDWIAFTAYGTSTAWRHLAAGNDLDDPGRLQPGQRLLIV
                     PLES"
BASE COUNT          313 a          806 c          795 g          304 t
ORIGIN      
        1 tccgctggtc gcgccgcagc cggtgagcgg gctgggcggc ttcctgagcg ccatcgttaa
       61 ccgcgtactg ggtactacgc ctgcgccacc tgcgcccgtc gccggcccgg cgccgctcga
      121 tatgccctgg gcgcggcgcc ccgcggcgcc cgctgtggaa gaagacagcg ccggggagta
      181 tgaaccggcc cccgcgcctc tctctgtgca gcgggccgtt gccgcgccgc ccgcgccgcc
      241 cgctggcgcc accgccgtag ccgcgctgcc cccgacggca gcggccccgg ttgccgattc
      301 cggccccgcg gtggctatgc ccgccgtccc aacgtccggc gatccgtttg cggtgccggt
      361 gccgggcgac ctgcccgccg ttcccgcagc gcccgcctgg gtgagcaccc ctgcgccggc
      421 cttcgatcag actgccggag agctcgctga agcgccggtc gcgcagccct ggatggatct
      481 cgactcgctc ggcgatgtgc tggcgcgggt ccagactccg ctggtcccca cagcgggcct
      541 gagcacggcc gggcagggcg ggctgtggcg ccggatcgtg ggagctttcg agcgcgcgct
      601 gcccggtggc ggcgcgccgg cagcccccat gaccctggcc tggagcggcg gcagcccggc
      661 gacgaccggc gcgggtgccg cgccgtttgt gcccgcgcag ccgggcggtt atagcgctcc
      721 atccgcagga agcgccgccc cgtggccggt ctacgggccg ggcgccgggg cctggatacc
      781 ggctgcggga ccgggtgggg ccgccctgct ggccggtcgc ttgccggccg atttcggggc
      841 cgcgctgggc ccccacgccc tcacgcctgc cgtgccggtc ccatattttg acgccgagtc
      901 agcgggcgat gcggacgccg aagtgggcga tgccgcgcag gcctggatcg ccgccctcga
      961 ccggatgtac ggcgcgcccg ccgccgggtc ggagacgatg ccgctggccg tgccctatgg
     1021 cggctttgtc gtccccgccg ccggggcgag cggcgtgctg ccgcaagacc tggcctgggt
     1081 cgcggaaacc atcggcttgg agagtgcgcc gccgtctgcc accccggcgc gccgggtgtg
     1141 ggagcgacct gtcgccgggg cgccggcccc gccgagttgg gcgggcggcc agggaccggg
     1201 cttcgtcccg ccggcgccgg gatggatcgg cgataccggg agcgccgagt acgatagcga
     1261 ggccgccgcc tgggccgatg tcgtagccgc cgccgtcgag agcgactacg gggccggggt
     1321 gcccgcgctg gcgctggccg gcgaggagca aggcgtgccc gcggtccagg acgccccttc
     1381 cggcgcggac catgaagccg cgggcgacgc agcgggcgac ctggacgaat tagccaattc
     1441 cgtgtacgag attatccgcc gccgcttgga ggtggaacgc gaacgtgact gggcctagcc
     1501 cggcgcaagg agggactgcc aatgcctgcc aataacggcc tccaggaagc tgtcatcaag
     1561 aacctgctca cgggcgagga agtgcgctgc cttttccgcc ccaaagagta caccttcgcc
     1621 aagcagaaca cctggacccc caatcgggct atcggcaaaa ccatgcagcc gcccaagtac
     1681 accggcggcc agccgatgac cttgcagatg gagttgctgt tcgacaccta tgaggagcgc
     1741 ggcaaccgcg atgtgcgccg ccgcaccgac ggcctctgga agatgatgaa agtggccgac
     1801 cagaagaagg acccgcagac caaaaagagc gagccgccgc atgtggagtt ccgctggggc
     1861 cgcacgtgga ccttcaaggc cgtcatcacc tcgttgcagc agaagttcac cctgttcgac
     1921 ccggacggca cgccggtccg ctccacggtg acgatctcgt tcatgcaggc cgaggagcag
     1981 ggccagtatc cggggcagaa ccccacgtcg ggcgcccgcg agggctacgc cgtccatgtg
     2041 gtcaaggagg gcgagaccat tgactggatc gccttcaccg cttacggcac cagcacagcc
     2101 tggcgccacc tggccgccgg gaacgacctg gacgatcctg gtcgcctgca accgggccag
     2161 cgcctcttga tcgtcccgct ggagagttaa gcgccatgcc tgcgaccgta cacatcgc
//
LOCUS       DASUYC010001127         2383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_926463,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001127 DASUYC010000000
VERSION     DASUYC010001127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926463"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(314..754)
                     /gene="ruvX"
                     /locus_tag="VF276_18410"
     CDS             complement(314..754)
                     /gene="ruvX"
                     /locus_tag="VF276_18410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX6608879.1"
                     /translation="MRILALDLGRKRIGVAVSDELCLVARTLTTLQARGRNADTAAIA
                     ALVAEQDAGRVIVGLPLHLSGETGAQAEKVQKFGAYLATQLSVPVEYWDERYTTVEAS
                     RLLTQSGVHPRKQRDQIDATAAAILLQSYLDSHPHLQSGAFGSA"
     gene            complement(796..>2383)
                     /locus_tag="VF276_18415"
     CDS             complement(796..>2383)
                     /locus_tag="VF276_18415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608880.1"
                     /translation="PAAVAVAPPPDAVAVAPPVERAIPAPRPRLRERPARPQAQKRTS
                     RRGGGLLVLLGVLLILLALSAIVVFNIGPALATATVQLQPRPGDSLGVVRVTVPVLTT
                     GSADAGRAGLAAPSLQVTATTTLTPTQPGAGDTVRMAQPVQAQKFAAAVVADKTIPTT
                     GVRQQPNKAAVGTIEFTNTSSAAVTIPANTVLTAPNGKKFHTVNTITVAGTNFLGRTF
                     GTGRVDIQANEPGPDSNGLRVGGSYGSAVFATVIPPGGGDTIPIQTIAEKDVADLQAA
                     LKGDIVARASQELLGKVPPAMQPITCTMLPAIPDTAFEASPLPAIGSDAGEIHGVMTA
                     TVDVYAFAPAEADHHAALAVQGAVPTDLPPGIDAQIDPASVDETALTLTQTSDCEGGR
                     VEYSTELRPRILYTVTDPKIIQDQIKQLVRGKTPAEARQLIASSPWGPYLDSAGVHIN
                     LGSSLLNMQQDTLPEAVTNINVQLPTQPGGTATGGGGDVTPTPAVAPAGTSEPPPAGA
                     PAGTDTPAPAAIPAGTGEPK"
BASE COUNT          329 a          797 c          865 g          392 t
ORIGIN      
        1 gtcattctga gcgcagcgaa gaatctccgt ctcgtgcggg ttagcgccga gactctgcgc
       61 taaaaagagg ctggacgcgt gcggcggcaa ggcgggtttg aaacccgccc ctacaccgcc
      121 atcccggttc ttgtctgccc ctacaccgcc agccagcctt gtccgcccct acatcgccat
      181 cccggccctt gtccgcccct ataccaccag ccggccttgt cggcccttac accaccagcc
      241 cggctctttt cagtagccct tgtggggcgg gaacccggat aggcgcaaag tgacagggga
      301 cggggacgga cggctaagcg gagccgaagg cgccggattg gagatgcggg tgggagtcga
      361 ggtaactttg cagcaggatg gcggcggcgg tggcgtcgat ttggtcgcgt tgcttgcgcg
      421 ggtggacgcc tgactgtgtg agcaggcgcg aagcctccac cgtggtgtag cgctcgtccc
      481 agtattcgac cgggacggag agttgcgtag cgaggtacgc gccgaacttc tggacctttt
      541 cagcctgggc gccggtctcc ccgctgaggt gcaggggcag gcccacgatc acgcgcccgg
      601 catcttgctc ggccaccagc gcggcgatgg ccgccgtgtc ggcgttgcga ccgcgggcct
      661 gcaatgtggt cagcgtgcgc gccaccaggc acagttcatc gctcaccgcc acgccgatgc
      721 gcttgcggcc aaggtcgagc gcgagaatac gcatgcgcga tcccggagcg ggcgggcgcc
      781 gcgccccgaa ccctcctact taggctcgcc ggttccggcg gggattgcag ccggtgccgg
      841 cgtatccgtg cccgcgggcg cgcctgccgg cggcggctcg ctggtgcccg cgggggcgac
      901 agccggtgta ggcgtcacgt cgccgccgcc gcctgtggcc gtgccgccgg gctgcgtggg
      961 tagctgcacg ttgatgttcg tcaccgcctc gggcagcgtg tcctgctgca tattcaacag
     1021 gctagaacca aggttgatat gcacaccagc actatcgaga tacggccccc agggcgagga
     1081 cgcaataagc tgccgggctt cggcgggcgt cttgccgcgc acaagctgct tgatctgatc
     1141 ctggataatc ttcgggtccg tcaccgtgta gagaatccgc gggcgcaact cggtgctgta
     1201 ttcgacccgc ccaccctcgc agtcgctggt ctgggtcagg gtcagcgcgg tctcgtcgac
     1261 gctggccgga tcgatctggg catcgatgcc cggcggcagg tcggtgggca cggcgccctg
     1321 caccgccagc gccgcgtgat gatcggcctc cgccggggcg aaggcgtata cgtccaccgt
     1381 cgccgtcatg acgccgtgga tctcgccggc atccgacccg atcgccggca atgggctggc
     1441 ctcgaaggcc gtgtcgggga tcgcgggcag catggtgcag gtgatgggct gcatcgcggg
     1501 cggcaccttg cccagcagtt cctggctggc gcgagccacg atgtcgccct tgagcgcggc
     1561 ctgcagatcg gcaacgtcct tctcggcaat cgtctggatc gggatggtat cgccgccgcc
     1621 cggcgggatg accgtggcga agacggcgct gccgtagcta ccgcccacgc gcaggccgtt
     1681 ggagtccggt cccggctcgt tggcctggat gtccacccgc ccggtgccga aggtgcggcc
     1741 caggaaatta gtgcccgcga cggtgatggt gttcaccgtg tggaacttct tcccgttcgg
     1801 cgcagtcagc acggtgttcg ccgggatggt cacggcggcg ctgctcgtgt tggtgaactc
     1861 gatggtgccg accgcggcct tgttgggttg ctggcggacg ccggtggtcg ggatcgtctt
     1921 atcggcgacc acggcggcgg cgaatttctg cgcctgcacc ggctgggcca tgcgcaccgt
     1981 gtcgccggcg cccggctggg tgggggtcag cgtcgtggtc gccgtgactt gcagcgacgg
     2041 cgccgccagc ccggcccggc ccgcatcggc gctgccggtg gtcagcaccg gcacggtcac
     2101 gcggaccacg cccagggagt cgccggggcg cggctggagt tgcacggtag ccgtagccag
     2161 tgccgggccg atgttgaaga cgacgatagc gctgagggcc agcaggatga gcaacacgcc
     2221 cagcagcacc agtaaccccc ccccgcgccg cgaggtgcgc ttctgggcct ggggccgggc
     2281 tgggcgttcg cgcaggcgcg gccgtggcgc cgggatggcc cgctcaaccg gcggggccac
     2341 cgccacggcg tcgggaggcg gcgcgactgc cacggcggcg gga
//
LOCUS       DASUYC010001128         3760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_2044334, whole genome shotgun sequence.
ACCESSION   DASUYC010001128 DASUYC010000000
VERSION     DASUYC010001128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2044334"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2173)
                     /locus_tag="VF276_18420"
     CDS             complement(<1..2173)
                     /locus_tag="VF276_18420"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6608881.1"
                     /translation="MLHTRNHGAFPVFARAGTLALALAMALTALPVQPSMAAPGQPQA
                     QKELIFVSDGMRPDLAEKWAQSGDMPNYAKIFNNGVTGDNGMVPQFPPNTGAGWSSIS
                     TGTWSGTHGAVNNTFHIDSNAITTSTTAYNAALLQAETYGEVAEKMGKKVAIMDWPAT
                     LPGTKVKGPAVDFRNFYSNRGVASNFDVPGVLPDFVKSLGVGYTNTLQISDAAGWTGA
                     PQSFSPAKETSFSFNTYAISGTVAALNWPVYIYDSTNDNQVNYDKAVIAKGSKDAGQA
                     VANLKAGDWADIKLNLPQNNLLVGYYLKLMDLSPDLSKFRVYFTSLARARSNMPDLEQ
                     KIAVDFPSATGADFAPIEAGLIDGQTYVEQGLKWYDTFRMVHDYVIQTYQPDVVMAGY
                     PTTDEFSHQFMALSSPSYTGPRPAYGVDPATAEGYIKQAYKQADAALGHLWDLLGANT
                     ITFVASDHGFGAAYRSANVLQVLQDAGLYDPANRGKSQTGAYVAGGTANIYVNLKGRE
                     QGGVVEQADYEKVRSQIMDAFNNLSDNGVKITAAVMKKEETRTIQAGGYTINGWFPTR
                     TGDVVVVLQPPYQWDGATPGQRVADSAFYGQHGYLPDTVDLGKNVNMHAMFGIYGPGV
                     AQGKKIGKPRTVDLAPTAAYAIGIAPPRYAEGRILTEAFTDGSANLVPIQVLAWGDYH
                     GQLDPIQARVDGLTPLSGGVAAIGTYWKEARATNPNGTIILS"
     gene            2691..>3760
                     /locus_tag="VF276_18425"
     CDS             2691..>3760
                     /locus_tag="VF276_18425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX6608882.1"
                     /translation="MRREDLLLRTKLSPPEQHRRVLPRPALLARLREAFEYRLTIVQA
                     GTGYSKTTALAALDTGAFPLFWYSIGETDADPQRFLSYLVAAFRQRLPDLSDLPLAVL
                     QERGAEGSREAWMQALDALINALSEALPGPALLVVDDYHIVAGLPEIQALAEHFLTYL
                     PPHLHVVLSTRYPLAGPGLLRWRARGEVLDINRGALAFRPDEIEALFRETYDMPLAPA
                     ELATLAEKTEGWPIALQLVRQGLRSGTARRPADLLAQGSTSLFDYLARDVLGGQDPAV
                     AAFLRETAVLRELAPAACDAVRGAGDSAALLARLHDLDLFVVDLGAGHYRYHHLFHDF
                     LREQAAADPAGTQARHRRAAAY"
BASE COUNT          514 a         1241 c         1334 g          671 t
ORIGIN      
        1 cgctgaggat gatcgtgccg ttggggttgg tggcccgcgc ttccttccag taggtgccga
       61 tggccgccac gccgccgctg agcggggtga ggccgtccac gcgcgcctgg atcgggtcca
      121 actggccgtg atagtcgccc caggcgagca cctggatcgg caccaggttg gcgctgccgt
      181 cggtgaaggc ctcggtcagg atgcgaccct cggcgtagcg tggcggggcg ataccgatgg
      241 cgtaggccgc ggtgggcgcc aggtcgacgg tgcgcggctt gccgatcttc ttgccctggg
      301 ccacgccggg accatagatg ccgaacatgg cgtgcatgtt cacgttcttg cccaggtcca
      361 cggtgtcggg caggtagccg tgctggccgt agaaggccga gtcggccacg cgctggccgg
      421 gcgtcgcgcc gtcccactgg tacgggggct gcaacacgac gaccacgtcg ccggtgcgcg
      481 tcgggaacca gccgttgatg gtgtagccgc cggcctggat ggtgcgcgtt tcttccttct
      541 tcatgacggc ggcggtgatc ttgacgccgt tatcggacag gttgttgaag gcgtccatga
      601 tctgcgagcg gaccttctca tagtcggcct gctcgaccac gccgccctgt tcgcggccct
      661 tcaggttgac gtagatgttg gccgtgccgc cggccacata ggcgccggtc tggctcttgc
      721 cgcggttggc cgggtcgtag aggccggcgt cctggagcac ctggagcacg ttggcgctgc
      781 ggtaggccgc accgaagccg tggtcggagg ccacgaaggt gatcgtgttg gcgccgagga
      841 ggtcccacag gtggccgagc gcggcgtcgg cctgcttata ggcctgcttg atgtagcctt
      901 ccgccgtggc cgggtccacg ccgtaggccg ggcgcgggcc ggtgtagctc ggcgaggaga
      961 gggccatgaa ctggtgcgag aactcgtcgg tggtcgggta accggccatg accacatcgg
     1021 gctgataggt ctggatgacg tagtcgtgca ccatgcggaa ggtgtcgtac cacttcagcc
     1081 cctgctcgac ataggtctgg ccgtcgatca ggcccgcttc gatgggggcg aagtcggcgc
     1141 cggtggcgct ggggaagtcc acggcgatct tctgctccag gtcgggcatg ttgctgcgcg
     1201 cccgcgccag ggaggtaaag tagacgcgga acttcgacag gtcggggctg agatccatta
     1261 acttgaggta atagcccacg agcaggttgt tctggggcag gttgagcttg atatcggccc
     1321 agtcgccggc cttgaggttg gcgaccgcct gcccggcgtc cttactcccc ttcgcgatga
     1381 ccgccttgtc gtagttcacc tggttgtcgt tggtcgagtc gtagatgtag accggccagt
     1441 tcagcgccgc caccgtgccg ctgatggcgt aggtgttgaa gctgaagctg gtctccttgg
     1501 ccgggctgaa cgactggggc gccccggtcc agcccgcggc atcgctgatc tgcaaggtgt
     1561 tggtgtagcc gacgcccagc gacttgacga agtcgggcag cacgccgggc acgtcgaagt
     1621 tggaggccac gccgcggttg ctgtagaagt tgcggaagtc caccgccggg cctttgacct
     1681 tggtgccggg cagggtggcg ggccagtcca tgatcgccac cttcttgccc atcttctcgg
     1741 ccacttcgcc gtaggtctcg gcctgcaaca gcgcggcatt atacgccgtg gtgctggtgg
     1801 tgatggcgtt gctgtcgatg tggaacgtgt tgttcaccgc gccgtgggtg ccgctccacg
     1861 tgccggtgct gatgctgctc cagccggccc cggtgttggg cgggaactgc ggcaccatgc
     1921 cgttatcgcc ggtgacgccg ttgttgaaga tcttggcgta gttgggcata tcgcccgatt
     1981 gcgcccactt ttccgccagg tcgggccgca tgccgtcgga aacgaagatc aactccttct
     2041 gggcctgcgg ttggccgggc gccgccatgc tgggctgcac cggcagggcc gttagggcca
     2101 tcgcgagggc cagcgccagc gtgccggcgc gcgcgaaaac ggggaacgcg ccatggttcc
     2161 gggtatgaag cattcagact cctccttcca gggtatcagg gacaatccgt acagcggtga
     2221 tccaccatgc cggggactca tgaatcgaag tcgcccgcag gaaccgatta tagcacagtg
     2281 gagccagtgt tggggcgtga aacggcacca ataggaacga agcgcactca gctatagtga
     2341 tgattttcga tccgcgaatt ttgcatatac gttgccggga tgatcgggcg ggtgcgcgcc
     2401 atcgttttgc atcccggcgg gcggggcaac ctctcccccg gcccctcccc gcggcgggga
     2461 ggggagtcgc tttcccgcgc gccggcgatt ccccccttcc cgcggcggga agggggccgg
     2521 ggggttaggt tggtcgccta ccccgatccc gcaaccccgc ttgcggaatc gcgcggcatg
     2581 ggcgataatg ccggggagcg ccccgcgggt gcggcgatcc cgcatccggt gcgagggcgg
     2641 tggggtgctc gcgccggagg accggcccgc cgccaggaga ccccgcccgt atgcgccgcg
     2701 aagacctgct gctccgtacc aagctctccc cgcctgagca acaccgccgg gtgctgcccc
     2761 ggcccgccct gctggcccgg ttgcgcgagg ccttcgagta ccggctcacc atcgtccagg
     2821 cgggcaccgg ctacagcaag accacggcgc tggccgcgct cgacacgggc gcgttccccc
     2881 tgttctggta cagcatcggc gagaccgacg ccgacccgca gcgcttcctg tcgtacctgg
     2941 tggccgcctt ccgccagcgc ctgcccgatc tgtccgacct gccgctggcc gtgttgcagg
     3001 agcgcggggc cgagggcagc cgcgaggcgt ggatgcaggc gctcgacgcg ctgatcaacg
     3061 cgctcagcga ggcgctgccc ggtccggcgc tgctggtggt ggacgactac cacatcgtcg
     3121 ccgggctgcc ggagatccag gccctggccg agcatttcct gacctacctg ccgccccacc
     3181 tgcatgtcgt gctgtccacg cgctatccgc tggccgggcc gggcctgttg cgctggcggg
     3241 cgcggggcga ggtgctggac attaaccgcg gcgcgctggc cttccgcccc gacgagatcg
     3301 aggcgttgtt ccgcgagacc tacgacatgc cgctggcgcc cgccgaactg gcgacgctgg
     3361 ccgaaaagac cgagggctgg cccatcgcgc tgcaactggt ccgccagggg ttgcgcagcg
     3421 gcacggcccg ccgccccgcc gacctgctgg cccagggctc gacttcgctg ttcgactacc
     3481 tggctcgcga cgtgctgggc gggcaggacc cggcggtggc cgccttcttg cgcgagaccg
     3541 ccgtgctgcg cgaactggcg cccgccgcct gcgacgccgt gcgcggtgcc ggcgacagcg
     3601 ccgcgctgct ggcccgcctg cacgatctcg atctgttcgt ggtcgacctg ggcgccgggc
     3661 actaccgcta tcatcacctg ttccacgact ttttgcgcga gcaggccgcc gccgatccgg
     3721 cgggcaccca ggcccggcac cgccgtgccg ccgcctattt
//
LOCUS       DASUYC010001129         2104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1583757, whole genome shotgun sequence.
ACCESSION   DASUYC010001129 DASUYC010000000
VERSION     DASUYC010001129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1583757"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VF276_18430"
     CDS             <1..372
                     /locus_tag="VF276_18430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608883.1"
                     /translation="HYVEEVYDLAVHPAADHHAAGAAPAEQVSAHQEPAAGHAATTGA
                     PDAHSATVATDHASAPDGHSAAAAPDHTSAPVAHQPAGLPQTGGNGATAGSPWAVWAL
                     AGLGLAGLGLGLRVARGRRAR"
     gene            491..979
                     /locus_tag="VF276_18435"
     CDS             491..979
                     /locus_tag="VF276_18435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608884.1"
                     /translation="MKPFIPQPITAEHEELHAELVRATQAGGQTGTAAAEVARLLHQH
                     FEAEEAYALPPLGYLAILAGDAPVGDVTEVLAMTHQLVDDLPNMLQEHRAIVAALQVL
                     VQAATDEHKPEHVRFAEKLMLHAQAEEEILYPAAILVGKYLELTAARPEAALHLEPAR
                     MA"
     gene            complement(1041..1622)
                     /locus_tag="VF276_18440"
     CDS             complement(1041..1622)
                     /locus_tag="VF276_18440"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX6608885.1"
                     /translation="MSDEPRPIPINVRGQKVALGPFLREHVAVHWGWLQDPEIAVHGN
                     GSFELRSLERGLDGFANPTKTEMVFSIYALEDLTLIGETVLMEIDHRQGTAMFGITIG
                     RKEYWGQGYGTEATRLVLDYGFRFLNLHNIMLITTSFNTRAQRAYAKAGFKEIGRRRG
                     SLLVGGKRYDDIYMDCLATEFESPRPGWFALPE"
     gene            complement(1684..>2104)
                     /locus_tag="VF276_18445"
     CDS             complement(1684..>2104)
                     /locus_tag="VF276_18445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase PknK"
                     /protein_id="HEX6608886.1"
                     /translation="VYALEGYASVAETTVTLWETGRHDPATARTDRRAAHRAVRALNN
                     FARVFPIAAPRAGRWRGLAAWLDGHPALARTVWARSLEAATRFAMPYEQALLHYEMGR
                     HARGRARRAHFAQARAGFARLGATGDLARLAAAARDP"
BASE COUNT          314 a          711 c          795 g          284 t
ORIGIN      
        1 cattatgtgg aggaggtcta tgacctggcc gtccacccgg ccgcggatca ccacgcggcg
       61 ggcgcggccc cggcggaaca ggtgagcgcg caccaggaac cggcggccgg ccatgccgcg
      121 acgacgggcg cgccggacgc ccacagcgcc acggtggcta ccgaccacgc gagcgcgccg
      181 gacgggcata gcgcggcggc ggcccccgac cacacgagcg ccccggtggc acaccagccc
      241 gccggcctcc cgcagaccgg cgggaacggc gccactgccg gcagcccctg ggcggtctgg
      301 gcgctggcgg gcctcgggct agcgggtttg ggcctcggcc tgcgggttgc gcgcgggcgg
      361 cgggcgcgct aggctcctcg tcccgtctct cggtgcgaac ccggatcgcg cgtcgcacgt
      421 tcgattgggt tctgcaccgg ggcatggcgg gtgtgcagac gacgtacaac agcagaaagg
      481 aaacaagacg atgaaaccgt ttataccgca accgatcacg gcggaacacg aggaactgca
      541 cgcggaactc gtgcgggcga cccaggccgg cgggcagacc ggcacagcgg cggcggaggt
      601 cgcgcggctg ctgcaccagc acttcgaggc cgaggaagcg tatgcgctgc ctccgctcgg
      661 ctacctcgct atcctggccg gcgacgcacc ggtcggtgat gtgaccgagg tactggcgat
      721 gacccaccaa ctggttgacg atctgcccaa tatgctgcag gagcaccgcg cgatcgtcgc
      781 cgcgctccag gtactggtgc aggccgcgac cgatgagcac aagcccgagc atgtgcgctt
      841 cgccgagaag ctaatgctcc acgcccaggc cgaagaggag atcctctacc cggccgccat
      901 cctggtcggt aagtacctgg aactgacggc cgcacggcct gaagcggcgc ttcacctgga
      961 gccggcgcgc atggcgtgac cccgccccag gcacctggcc cgcgtcgcga cccccgtccg
     1021 gcgcgggtcc ggtgcgccgc tcactccggc agggcgaacc agccgggccg gggcgactcg
     1081 aactctgtcg ccaggcagtc catgtagatg tcgtcgtagc gcttgccgcc gacgagcaac
     1141 gagccgcggc ggcggccgat ctccttgaag ccggccttgg cgtaggcgcg ctgggcgcgg
     1201 gtgttgaagc tggtggtaat taacataatg ttgtgcaggt tgagaaagcg gaagccgtaa
     1261 tccagcacca gccgggtggc ttcggtgccg tagccctggc cccagtactc cttgcgaccg
     1321 atggtgatgc cgaacatggc cgtgccctgg cggtggtcga tctccatcag caccgtctcg
     1381 ccgatgagcg tgaggtcctc cagggcgtag atactgaaga ccatctcggt tttggtcggg
     1441 ttggcgaagc cgtccaggcc gcgctccagc gagcgcaact cgaagctgcc gttgccgtgg
     1501 acggcgatct cggggtcttg cagccagccc caatgcacgg ccacatgctc gcgcaagaag
     1561 gggccaagcg cgaccttctg gccgcggacg ttgatcggga tcgggcgggg ttcgtcgctc
     1621 atgcgggggc gtctcccttc gggatgcagg aatgggcgcc gggcgcgcgc ccggcggggt
     1681 gggtcatggg tcgcgggccg ccgcggcgag gcgggcgagg tcgccggtgg cgcccaggcg
     1741 ggcgaagccg gcgcgggcct gggcgaagtg ggcgcggcgg gcgcgcccgc gggcgtggcg
     1801 acccatctcg taatgcagta gcgcctgctc gtagggcatg gcgaagcggg tggcggcctc
     1861 caggctgcgc gcccagacgg tgcgggccag cgccgggtgg ccgtcgagcc aggcggccag
     1921 gccccgccag cgcccggcgc gcggcgcggc gatggggaag acgcgggcga agttgttcag
     1981 cgcgcgcacg gcgcggtggg cggcgcggcg gtcggtgcgc gccgtggccg ggtcgtgccg
     2041 gcccgtttcc cacagcgtca cggtcgtctc ggcgaccgaa gcgtagcctt cgagggcata
     2101 gacc
//
LOCUS       DASUYC010001130         3330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_196418,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001130 DASUYC010000000
VERSION     DASUYC010001130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196418"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VF276_18450"
     CDS             <1..411
                     /locus_tag="VF276_18450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015622785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX6608887.1"
                     /translation="GGGEKVTLKLVARYADGCNVGGGNPDTIRHKVDVLHQHCATLGR
                     DPKEITVSTSVEDIHLLAPGETPDQAAASMPNQYTPEQYRKRFKIWTADETVAQIQRC
                     VDAGADYIIVYLAGLAHNTDMVGRFAQEVIPRFA"
     gene            complement(571..864)
                     /locus_tag="VF276_18455"
     CDS             complement(571..864)
                     /locus_tag="VF276_18455"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX6608888.1"
                     /translation="MICVHCGQELPDTANFCLHCGRRPAMGNIEWRWVHRYTRPIIGL
                     LILFIGFLLLMYFDVIPFNIDLLNTATRIFWILLVAWSITTLLWIWMWRRTHR"
     gene            complement(1007..1978)
                     /locus_tag="VF276_18460"
     CDS             complement(1007..1978)
                     /locus_tag="VF276_18460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020618600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HEX6608889.1"
                     /translation="MELTGLHHVSALTAHAPENLAFYTRILGMRLVKKTVNQDDTTSY
                     HLFYGDEVGNAGTELTFFDIPHAAATRPGVSSISRIALRVPDRAALAYWAERLAEHGV
                     EHDPVTEEAGRAVLAFRDFEGQRLALVDDSGASVPGGTPWAKSPTPAPVAIRGLGTVT
                     LTVRRLEPTERVLTEVMGFRRAGSYPAAEGPQREIVVFATGAGGPGAEVHVDERRELP
                     MEQLGHGGVHHVAFRVPTSAEHAAWLDRLKTARVPNSGQIDRFYFQSIYFREPNGILF
                     ELATDGPGFATDEDPAHLGERLALPPFLEPRRALIESRLQPLELAVS"
     gene            2367..2648
                     /locus_tag="VF276_18465"
     CDS             2367..2648
                     /locus_tag="VF276_18465"
                     /inference="COORDINATES: protein motif:HMM:NF023857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family antitoxin"
                     /protein_id="HEX6608890.1"
                     /translation="MAEIPSFANEEEAAAWFATHDTSPYMARLEEVTEPLPVVRSAAR
                     KKPVGLRLRSDYLDALKQVAARKGIPYQTLIQMWLVEKLRQEAPDLLDR"
     gene            complement(2665..>3330)
                     /locus_tag="VF276_18470"
     CDS             complement(2665..>3330)
                     /locus_tag="VF276_18470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6608891.1"
                     /translation="GADVALGLRDADSGADLAAQIEGMGRRALRLPMDVRRLDQVRAG
                     IDATVERFGRLDILVNNAGIAPGNPAELVREEDFDETLAVNLKGTFFASQAAGRVMIG
                     QRYGRIINLSSQAGFVALPTESVYCMTKAGIAHLTRCLAVEWAPHGITVNAVAPTFIQ
                     TPGTVRWLEDPVNAADVVSRIPLGHVGRPVDVAGAIVFLASPAASLITGATLLIDGGW
                     TAR"
BASE COUNT          570 a         1049 c         1169 g          542 t
ORIGIN      
        1 ggcggcggcg agaaggtgac gctgaagctg gtcgccaggt acgccgacgg ctgcaacgtg
       61 ggcggcggca accccgacac gatccggcac aaggtggacg tgctgcacca gcactgcgcc
      121 acgctgggcc gcgaccccaa ggagatcacc gtcagcacga gcgtggagga catccatctg
      181 ctggcccccg gcgagacccc ggaccaggcg gcggcctcga tgccgaacca gtataccccg
      241 gagcagtacc gcaagcggtt caagatctgg acggccgacg aaaccgtcgc gcagatccag
      301 cggtgcgtgg acgccggggc cgattacatc atcgtctacc tggccgggct ggcccacaat
      361 accgatatgg tgggccggtt cgcccaggag gttattccgc ggttcgcgta acgcgggggg
      421 aaacctctcc ccccggcccc tccccgccgc ggggagggga gcgtattttc ctcgcaccag
      481 agattcctcc cttcccgcgg cgggaagggg ggtagggggg ttaggttggt cgggtgcccg
      541 gatgttcgcc cctacaatac gtggtatgga ctaccggtgg gtacgcctcc acatccatat
      601 ccacaaaagc gtagtaatgc tccaagctac taataatatc cagaaaatac gggtagccgt
      661 attgagcagg tcaatattga aagggataac gtcgaagtac atcagcagca ggaaaccgat
      721 aaagaggatc agcaggccaa tgataggccg cgtataccga tgcacccagc gccactcgat
      781 gttgcccatg gcggggcgcc ggccacagtg caggcagaag tttgccgtgt cgggtagttc
      841 ctggccgcag tgtacgcaga tcacgggtgc ctcctccggg cctatcaagg ccggtgctgc
      901 tcaattctta gcgtcgtgtc gggctatttt accataggta tacgcaaaac gggcgctacc
      961 ggtatcgcac cggtagcgcc cgttggctta gcaacgattg gcggggttag ctgacggcca
     1021 gttcgagcgg ctggaggcgg gactcgatca gggcgcggcg tggctccagg aagggcggca
     1081 gggccagccg ttcgcccagg tgcgccgggt cctcgtccgt ggcgaagccg gggccgtcgg
     1141 tggcgagttc aaacaggatg ccgttcggct cgcggaagta gatcgactgg aaatagaagc
     1201 ggtcgatctg gcccgagttc ggcacgcgcg ctgtcttgag ccggtccagc caggcggcgt
     1261 gttccgcgct tgtgggcacg cggaaggcga cgtggtggac gccgccgtgg cccaactgct
     1321 ccatcggcag ctcgcgccgc tcgtccacgt ggacctccgc gccgggaccg cccgcaccgg
     1381 tggcgaagac gacgatctcg cgctgcgggc cctcggcggc ggggtagctg cccgcccggc
     1441 ggaagcccat cacctcggtc agcacgcgct cggtcggctc cagccggcgc acggtgagcg
     1501 tgaccgtgcc caggccgcgg atggcgaccg gggccggggt gggactcttg gcccaggggg
     1561 tgccgccggg cacgctcgcc ccgctgtcgt cgaccagcgc gagccgctgg ccctcgaagt
     1621 cgcggaaggc gaggaccgcc cgacccgcct cctcggtcac cgggtcgtgc tcgacgccgt
     1681 gctcggccag gcgctcggcc cagtaggcca gcgcggcgcg gtcgggcacg cgcagggcga
     1741 tgcgcgagat gctgctcacg ccgggccgag tggccgccgc gtggggaatg tcgaaaaagg
     1801 tcagttcggt gcccgcgttg cccacctcat cgccgtagaa caggtgataa gaagtggtgt
     1861 cgtcctggtt cacggtcttt ttgaccaggc gcatgcccag gatgcgggtg taaaaggcga
     1921 ggttttcggg tgcgtgcgcc gtgagggcgg acacgtgatg taagccggtg agttccatgg
     1981 tttccttcca gtgtgtactg cttgtgccta tgcgttatcc ggcggaccgc cggcccgcgt
     2041 ctaacgtttg gcttaagaat accaaaccta tgtcccgcga agcgtccttt ggggcgtcct
     2101 agccggggcg cggcggcgcc cggcccgtca acgggcgggc taacgcgacg aagcccgcct
     2161 gcgcgggctg aagatgggtg cagattggtg cttgattctt gtctagacag cgtctatact
     2221 tctcggaata tcgccggtgg tgtggcttcc ggacaccttt cggatgcagc ttacactgac
     2281 aatgaacgag tacggcgcga taaatcgcgc ggctacgcat gagggagcgc ggtgcgctgt
     2341 aagttgcagt aggaggcgaa ggtaagatgg ccgaaatacc cagctttgcc aatgaggagg
     2401 aggccgccgc gtggtttgcg acgcatgaca ccagcccgta tatggctagg ctggaagaag
     2461 tgacggaacc cctgcctgtg gtgcggtcgg cggcccggaa gaagccggtg ggcctgcgcc
     2521 ttcgcagcga ctatctggac gcgctgaagc aggtggcggc acggaaaggc attccctacc
     2581 agacgctcat ccagatgtgg ctggtggaaa agctgcgcca ggaagcaccg gacctgctgg
     2641 atcggtagcc cgcaggtggc gcgcttaccg cgcggtccag ccgccgtcga tgagcagggt
     2701 ggcgccggtg atcagggagg cggcggggga ggcgaggaag acgatggccc cggccacgtc
     2761 caccgggcgc cccacgtggc ccagcgggat gcgcgagacg acatccgcgg cgttcaccgg
     2821 gtcttccagc cagcgcaccg tgcccggcgt ctggatgaag gtgggcgcga cggcgttgac
     2881 ggtgatgccg tgcggcgccc attccacagc caggcacctg gtcaggtggg cgatgcccgc
     2941 cttggtcatg caatagaccg actcggtcgg cagggccacg aagcccgcct gcgaactcag
     3001 gttgatgatg cggccatagc gctgcccgat catcacccgg cctgcggcct ggctggcgaa
     3061 gaaggtgccc ttgaggttga ccgcgagcgt ctcgtcgaag tcctcctccc gtaccagttc
     3121 cgccgggttg cccggggcga tgccggcgtt gttcaccaga atgtccagcc gcccgaagcg
     3181 ttcgactgtc gcgtcgatcc ccgcgcgcac ctgatccagc cgcctgacgt ccatcggcaa
     3241 ccgcagcgcg cggcggccca tcccctcgat ctgcgccgcc aggtcggcgc cggaatccgc
     3301 gtcccgcagg cccagggcga cgtcggcgcc
//
LOCUS       DASUYC010001131         3682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1583847, whole genome shotgun sequence.
ACCESSION   DASUYC010001131 DASUYC010000000
VERSION     DASUYC010001131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1583847"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VF276_18475"
     CDS             complement(<1..431)
                     /locus_tag="VF276_18475"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017580822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX6608892.1"
                     /translation="MLGPLHPTPYADVNDALRDLLAGVRAALDMRFVGMYLTGSLALG
                     DFDPATSDIDLVVVTGDALPANLVEPLREMHERFAAGDSPWAGKVEAAYIPRNALRRA
                     APDDALYPQVEKDRDLFVDQLESGWIVQCYILREHGVTLAGP"
     gene            complement(560..763)
                     /locus_tag="VF276_18480"
     CDS             complement(560..763)
                     /locus_tag="VF276_18480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608893.1"
                     /translation="MDEPLLTQPGGPALEAPPTDTAAGYGEAGRLPIVPPQPDGTGLD
                     DDLALDSPWPTVGGWWMLAGDGD"
     gene            complement(782..2581)
                     /gene="polX"
                     /locus_tag="VF276_18485"
     CDS             complement(782..2581)
                     /gene="polX"
                     /locus_tag="VF276_18485"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HEX6608894.1"
                     /translation="MPGKSAKPKKDLTPPPDLQPVPGINNMSLAETMSEIAAMLELKG
                     ENRFKIQSYRRAADTLLNMPEDIRVVWRAGRLEDLPNIGEAISTKIDELLRTGRLGFY
                     DRLRAEIPPGVLALTAVPDIGPKTALALYQSLGISSIAELETALNEGRIRTVPGFGER
                     SEEKLRESLAAVQRRAANQRVLLAEARPLARQLVGQLQESGLPITRIEVAGSIRRGRS
                     TIGDMDILCTSPDPAQVINFFVGLPLVGEVVSHGGNKSTVRLRNGMQVDLMVLPPEHF
                     GALLHHFTGAREHNIQMRDRALNRGLRLNEYGFDRPDGTRILGPQEEDVFATLGLPWI
                     PPELREGAGEIEAAAAGRLPNLVTVADIKGDLHNHSKWSDGTTTIEAMARAAQARGYQ
                     YMAITDHSQSLTIARGMTVDQIRAQAAEVAEVNRKLAPFRVLHGVELEIKTDGTLDYP
                     DEVLAELDIVIASAHQGLRHDPERVTARVLDACRNPHVDIIAHPTGQILNSREPSGLD
                     VEALIETARETGTALEINASPERLDLNEVYTRRAVESNVLLSIDTDSHHPDGFANIEY
                     GITVARRGWAEAPNILTTWPLERVLAWLARRGA"
     gene            complement(2721..3527)
                     /locus_tag="VF276_18490"
     CDS             complement(2721..3527)
                     /locus_tag="VF276_18490"
                     /inference="COORDINATES: protein motif:HMM:NF021082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2383 domain-containing protein"
                     /protein_id="HEX6608895.1"
                     /translation="MSDKELGKTQKALEELVKVEYQAIAAYDEGIAETEDNKLRRQYT
                     RFRNEHEKQARALNNRLAELGGEPVEYGAGTGKVKAGLFAKIAGMFGDAASIGGMVKG
                     AEDGIRIYLDHLDEIHDSKALSIIRRNLESKQQEVRWLEEQSQKASEDKGADMSERVQ
                     KAQDTAGEVLKQQAKALKETKSKVEETIQPESKKTGFLGLPVWLLLAAAGAAAFFFLR
                     RQEEPDFSDEAFQYETTDFSVAGENPSGEGYHGINESSDTTQSMGTDGNA"
BASE COUNT          515 a         1200 c         1258 g          709 t
ORIGIN      
        1 gggccggcca gcgtcacccc atgttcgcgg aggatgtagc attggacgat ccagccgctt
       61 tcaagctggt ccacgaagag gtcgcggtcc ttctcgacct gcggatatag ggcatcgtcc
      121 ggcgcggcgc gccgcagggc gttacgcggg atgtaggcgg cttccacctt gccggcccag
      181 ggggagtcgc ccgcggcaaa gcgctcatgc atctctcgca acggctcgac aagattcgcg
      241 ggaagcgcgt cgccggtcac aacgacaaga tcgatatcgc tggtggcggg atcgaagtcg
      301 ccgagggcca gcgatccggt gaggtacatc ccgacgaacc gcatgtccag ggcggctcgc
      361 acccccgcca gcaggtcgcg cagcgcgtca tttacgtccg cgtatggtgt cgggtggagg
      421 ggtccaagca tgagctaccg ggcggctact caccgggcta accagcaagc cgcctccctt
      481 ccccgcgcag ggaagggaca gaatgttaaa ccacttgtct ccccttcccg caccgggaag
      541 gggtcggggg ttaggccgtc tagtcgccgt cgccggccag catccaccag ccgcccaccg
      601 tgggccaggg gctgtcgagc gcgaggtcgt cgtcaaggcc ggtcccgtcg ggctggggcg
      661 ggacgatggg cagccgcccg gcctcgccgt agccggcggc ggtgtcggtg gggggcgctt
      721 cgagggccgg cccgcccggt tgcgtgagca gaggttcgtc catggtcggt tctcccgcgg
      781 gtcaggcgcc gcgccgcgct agccaggcca gtaccctttc gagcggccag gtagtcagga
      841 tattgggtgc ttccgcccag ccgcgccggg cgaccgtgat gccgtattcg atgttggcaa
      901 agccgtcggg atgatgcgag tccgtatcga tagataacag cacatttgat tccacggcgc
      961 ggcgggtata gacctcgttc aggtccaggc gctctggcga ggcgttgatc tccagcgccg
     1021 tgccggtttc gcgcgccgtt tcgatcaggg cttccacgtc gaggccgctg ggttcgcggc
     1081 tgttgaggat ctggccggtg gggtgggcga tgatatcgac gtgcggattg cggcaggcgt
     1141 cgagcacgcg cgccgtgacc cgctccgggt cgtggcgcag accctggtgg gccgaggcga
     1201 tcacgatgtc gagttcggcc agcacctcgt cggggtagtc gagcgtgccg tcggttttga
     1261 tttcgagttc gacgccgtgc agcacgcgga agggcgccag cttgcggttg acttcggcta
     1321 cttccgccgc ctgggcgcgg atctggtcca cggtcatgcc gcgggcgatg gtcaggctct
     1381 ggctgtggtc ggtgatcgcc atgtactggt agccgcgcgc ctgggccgcc cgcgccatcg
     1441 cctcgatggt ggtggtgccg tcgctccact tgctatggtt gtgcaggtcg cccttaatgt
     1501 cggccaccgt gaccaggttg ggcaaccgcc cggcggcggc ggcctcgatc tcgcctgcgc
     1561 cctcgcgcag ttcgggcggg atccagggca ggccgagcgt ggcgaacacg tcttcttcct
     1621 gcgggccgag gatgcgcgtg ccgtcgggcc ggtcgaagcc gtattcgttg agccgcaggc
     1681 cccggttcag cgcccggtcg cgcatctgga tgttgtgctc gcgcgcgccg gtgaagtggt
     1741 gcagcagcgc gccgaaatgc tccggcggca gcaccatcag gtctacctgc ataccgttgc
     1801 gcaggcgcac cgtcgatttg ttgccgccgt gcgacaccac ctcgcccacc agcggcaggc
     1861 ccacgaagaa gttgatcacc tgcgccgggt cggggctggt gcagaggatg tccatatcgc
     1921 cgatggtcga gcggccgcgg cggatcgacc cggccacctc aatgcgggtg atgggcaggc
     1981 cgctctcctg gagctggccg accagttggc gggccagggg ccgggcctcg gccagcagca
     2041 cccgctggtt ggcggcccgg cgctgcacgg cggccaggct ttcgcgtagc ttttcctcgg
     2101 agcgctcgcc gaagccgggc accgtgcgga tccggccctc gttcagcgcc gtctccagtt
     2161 cggcgatgct gctgatgccc aggctctggt aaagcgccag cgccgtcttc ggcccgatgt
     2221 cgggcacggc ggtgagcgcc agcacgcccg gcgggatctc ggcccgcagc cggtcgtaga
     2281 agcccagccg cccggtgcgc agcagttcgt cgatcttggt cgagatggct tcgccgatgt
     2341 tgggcagatc ttccagccgc ccggcccgcc agaccacccg gatgtcctcg ggcatgttga
     2401 gcagcgtgtc ggccgcgcgg cggtacgact ggatcttgaa gcggttttcg cccttcagtt
     2461 cgagcatcgc cgcgatctcg ctcatcgtct cagccaggct catgttgttg atgcctggga
     2521 ccggttgcag atcggggggc ggggtcagat ccttcttcgg cttggcggat ttacccggca
     2581 tacctgcggt cctcctgcgg gggcggctgg atgaagataa cacaagcgcc gccccggaag
     2641 gcggcgtttg tgctgttaga cgccaggcgg cgggccggtt tgaaaccggc tcctacaccg
     2701 caaaactacg gggttagtta ctaggcgttg ccgtccgtgc ccatgctctg ggtggtgtcg
     2761 ctgctctcgt taatgccgtg gtagccttcg ccgctggggt tctcgcccgc gacgctgaag
     2821 tcggtcgtct cgtactggaa ggcctcatcg ctgaaatccg gctcttcctg gcggcgcagg
     2881 aagaagaagg ccgccgcgcc ggccgccgcc agcagcaacc acaccggcag ccccaggaag
     2941 ccagtctttt tggactcggg ctggatcgtt tcctcgacct tgctcttggt ttccttgagc
     3001 gccttagcct gctgcttgag cacctcgccg gcggtatcct gagccttctg cacgcgctcg
     3061 ctcatgtcgg cacccttgtc ctcgcttgcc ttctgcgact gctcttccag ccagcgcact
     3121 tcctgttgct tggattccag gttgcgccgg atgatgctca gcgccttgct gtcgtggatc
     3181 tcgtccaggt ggtcgaggta gatgcggatg ccgtcctcgg cacccttgac catgccgccg
     3241 atgctggccg cgtcgccgaa catgccggcg atcttggcga acagcccggc cttcaccttg
     3301 cccgtgcccg cgccgtactc gaccggctcg ccgcccagtt cggccaggcg gttgttgagg
     3361 gcccgcgctt gcttttcgtg ctcgttgcgg aagcgcgtat actgccggcg cagcttgtta
     3421 tcttcggtct cggcaatacc ctcgtcatag gcggcgatgg cctgatattc gaccttcacc
     3481 agttcttcga gggctttctg ggtcttcccc agttccttgt cgctcatcaa tatagcctcc
     3541 tatcggtaaa tcacaccgtc catccgtgca cagcacaggt gtcctggtcg agcgcgtacc
     3601 gcccgcgcaa atgtaggcca ggggatgggc agggcggtag caagtcccgt gcaatgtcag
     3661 caagagacgt gccagtaaag cg
//
LOCUS       DASUYC010001132         3071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_2069893, whole genome shotgun sequence.
ACCESSION   DASUYC010001132 DASUYC010000000
VERSION     DASUYC010001132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2069893"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(352..1086)
                     /locus_tag="VF276_18495"
     CDS             complement(352..1086)
                     /locus_tag="VF276_18495"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX6608896.1"
                     /translation="MKTQQDQDTDRPGTAQKTPPVQQGAAAPAPPGVRVLRRVVRGIF
                     GLVFRVRVVGLAQVPATPVIICANHLGWADTFLVLLFFPVEPRVYVLGEQEVRTISRW
                     RTWLIDRLEIMVALDRRNPLRAVRTMDDVLRRGGSLLIFPEGQLGATEGALQPLAEGA
                     ARASLVTGAPLLPVGLTGTRELWLRRPLTMRIGPPIAPASVTGTTRERVDALTPRLRT
                     AMLALLPGDPARPRWKPLRAWLTKLF"
     gene            complement(1334..2641)
                     /locus_tag="VF276_18500"
     CDS             complement(1334..2641)
                     /locus_tag="VF276_18500"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6608897.1"
                     /translation="MDMPIRDTTFVPPGAPVEPEVAAPAVVERFNLGLALRYNVANLG
                     AQVVYALFNFGMPLYLDTYRLNPSLIGLLANERSFVASFVQPLVGRLSDRTRSPLGRR
                     RPFFLAGIPLMALALVALALHPPFWIMLAIMSVAAFFLSVAWDPYMAFMADLFPPEHR
                     GRVGGLLGIGSGLGTVIFLLLAFNLWEHAEFAVFLICIAVMVAAWAFTFFTVKEPPLP
                     PAEAIAARAARSRPNPAAYVRDVARYPEVVKYMLAVNFYWLGLGGVTPFITLFGVKAL
                     GATEQQAFLLPLIATLLNALFAVPAGYLADRFSKKAVMTTGLLIIGVVAMIGSQSQTL
                     WQAMIALGAVGVGNATLAQINPLLADLVPRKRTAEFMGLVSAVFSFAQPLGSVAAGAV
                     VSLATIWVGGNDAYRWAFIFAGCTLLLSAALLQTVKPAQAVLD"
BASE COUNT          558 a         1001 c         1030 g          482 t
ORIGIN      
        1 tgctcatgct gcctccttat ttaccggtat ataatataat aaacgaaaca cggagtaaag
       61 tcaatagttt gttcaaggat tcacccaagt tccatgggta tcgggatgga tgcagaggat
      121 tggggagagt gggtacccct accggccttg tccagggagc gcagcaaagt gtctcgtctt
      181 atgaccaaac gagacgcttc cctgcggtca gaatgacagt tccactgcca cccttgtcac
      241 tctgagcgca gcgaagagtc tcgtcgcgtg cctaatcgag acgagattct tcgctgcgct
      301 cagaatgaca atttcagctt aaagcagtat tgatagcgaa cacaagggcg atcagaagag
      361 cttggtcagc caggcgcgca ggggtttcca gcgcgggcgg gcggggtcgc ccggcagcag
      421 ggccagcatg gcggtgcgca ggcggggggt cagcgcatcg acccgctcgc gggtggtgcc
      481 ggtgacgctc gccggggcga tgggtgggcc gatgcgcatg gtcagcgggc ggcgcagcca
      541 gagttcgcgg gtgccggtga ggccgacggg cagcaacggg gcgccggtga cgaggctggc
      601 gcgggccgcg ccctcggcca ggggttgtag cgcgccctct gttgcgccga gttggccttc
      661 ggggaagatg agcagcgagc cgccgcgccg cagcacatcg tccatcgtgc gcacggcgcg
      721 cagcgggttg cggcggtcca gcgcgaccat gatctccagg cggtcgatca gccaggtgcg
      781 ccagcgagaa atggtgcgca cttcttgctc gccgaggaca tagacccgcg gctcgactgg
      841 gaaaaagagc agcaccagga aggtatcggc ccagcccagg tgattggcgc agataattac
      901 gggcgtggcc ggcacctgcg ccagcccgac cacgcgcacc cggaacacca ggccgaagat
      961 gccgcgcacc acccgccgca gcacccgcac ccccggcggc gcgggcgcgg cggccccttg
     1021 ctgcacaggg ggcgtttttt gagcggtgcc ggggcggtcc gtgtcctggt cctgctgtgt
     1081 cttcatcggg ctgtgatcct ctctagccgg cgctgtaggg gccgatttca aaccggcccg
     1141 ccgcccaccg gtcacgctaa tttcgcgcta tgggggctgg tttgaaaccg gcctgccggg
     1201 cactgaccaa gctaatttcg cgttgtacgg gccggtggca aaccggcctg ccgggaactt
     1261 gccgagctaa tttcgcgttg caggggccgg ttgcaaaccg gcccccggta atcacaagcg
     1321 gaatggcgga cgattagtcg agcaccgcct gggcgggctt cacggtttgc agcagcgccg
     1381 cgctcagcaa cagcgtgcag ccggcgaaga taaaggccca gcggtaggca tcgttgccgc
     1441 ccacccagat cgtcgccagt gagacgacgg cccccgccgc caccgacccc agcggttgcg
     1501 cgaacgagaa gaccgccgac accaggccca tgaactcggc ggtgcgcttg cgcggcacca
     1561 ggtcggccag cagggggttg atctgcgcca gtgtcgcgtt gcccacgccc acggcgccca
     1621 gcgcgatcat cgcctgccac agcgtctggc tctgcgagcc gatcatggcg accacgccga
     1681 tgatcagcag cccggtggtc atgacggctt tcttgctgaa gcggtcggcc aggtagcccg
     1741 cgggcacggc gaacagcgcg ttgagcagcg tcgcgatcag cggcagcaag aacgcctgct
     1801 gctcggtcgc gcccagcgcc ttcaccccga acagggtgat gaacggggtc acgccgccga
     1861 ggccgagcca gtagaagttc accgccagca tgtacttgac cacttcggga tagcgcgcca
     1921 cgtcgcggac gtaggccgcc gggttggggc ggctgcgcgc cgcacgcgcg gcgatcgctt
     1981 cggcgggcgg cagcggcggc tctttcacgg tgaagaaggt gaacgcccag gccgccacca
     2041 tcacggcaat gcagatcagg aagacggcga actcggcgtg ctcccagagg ttgaacgcca
     2101 gcagcaggaa gatgaccgtg cccaggccgc tcccgatgcc gagcaacccg cccacgcgcc
     2161 cgcggtgttc gggcgggaac aggtcggcca tgaacgccat gtaggggtcc caggccaccg
     2221 acaggaagaa cgcggccacc gacatgatgg cgagcatgat ccagaacggc ggatgcagcg
     2281 ccagggcgac cagcgccagg gccatcagcg ggatgcccgc caggaagaag ggccgccgcc
     2341 ggcccagggg gctgcgcgtg cggtcgctga gccgcccgac cagcggctgc acgaacgagg
     2401 cgacgaacga ccgctcgttc gccagcagcc cgatcagcga ggggttgagc cggtaggtat
     2461 ccaggtagag cggcatcccg aagttgaaca gcgcgtagac gacctgcgcc cccaggttcg
     2521 ccacgttgta gcgcaaggcc agccccaggt tgaagcgctc gaccaccgcg ggcgccgcga
     2581 cttcgggttc gaccggtgcc cccggcggca cgaaggtggt gtccctgatt ggcatgtcca
     2641 tggtcttatc cttccacgac gtgtccccca tgcgccccat gtgcctctcg cgccaactat
     2701 ccgtatcata acaccggcgg tctcattcgc aatgggcaga tttgcgtaat ttgctcgatc
     2761 tccctgatcg aggcggctag gtagtttcgc ctatttaacc gcagatgtag tgatcgtaac
     2821 gcaggtgggc tatgcagcca cggctgagag tgctatgata agagaggcat ggccccgttg
     2881 gcaaacccca tacccccgcc catcccggtc gccggcactc caacccaaag gagaggtcca
     2941 tggctacgac tactcacacc gggaccctta ccgtgccccg cccccactcc gcgtggcgcc
     3001 gcgcctggcg gattgccgcc ctgctgctgg cgctgctcat cctcggcggc gcgctatttg
     3061 tctactggtt g
//
LOCUS       DASUYC010001133         2143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_428147,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001133 DASUYC010000000
VERSION     DASUYC010001133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428147"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            324..908
                     /locus_tag="VF276_18505"
     CDS             324..908
                     /locus_tag="VF276_18505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608898.1"
                     /translation="MPETDPALAGLPPDAYTRVSELADEVVRMYGGTGPPFPILHLLA
                     ILEPVTLILPPLADGRDAGVYGYAFPVEFARYKRHAALCLDSNAPTREVFVTAFHEAG
                     HVLLHILPDRCAPGIPYARRAGPPLDPRHGQAEIEADLFARLTTMPAAWVRPAWLYAA
                     AVTATPDEAVAWMADLFSVPVGWMQRRLLHLGLL"
     gene            complement(1161..1985)
                     /locus_tag="VF276_18510"
     CDS             complement(1161..1985)
                     /locus_tag="VF276_18510"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6608899.1"
                     /translation="MPAPNPRPADDPDAPADSGILDRFAPFYDLEYGAYDADLAFYRG
                     CAAQAVPRRSPAARVLELACGSGRVLLALAAAGHTCTGVDASPAMLALARARLAAAGL
                     PATLVAARMEALPPDLGPFDLVLCALNSFAYRPTQADQLATLVDVQARLRRGGLLVLD
                     LSPVDTGGPYPAGGELVHQGAWDRPGGGRVLKFVSGVWDSAEQQHHVHWIYDEEDAAG
                     RVRRTTIPQTLRYTYRWEAQLLLERAGLRLAGIYGTYDLDPYTSDSPRLILVAQRP"
BASE COUNT          325 a          693 c          765 g          360 t
ORIGIN      
        1 tgggtggccg gctgccggac ggcgccggcg ctgcgtcgac atcaggtagc ttggttatca
       61 tatttactgc ccatcctttg caccgaaaat cggtaacttg atcattatat gaaagttaga
      121 gcgctctaag tcaagagcat agtctatgcc ggtggcatct ggcggtgggc gacgagccgg
      181 gcgtgaacat ctgctacctg tgcggcgccg gaaaagtggc ggaatgctaa ccgttctgct
      241 tccggcatcc tcttgtaaaa ctagaacatt agtgctaaaa taggggcgct gtgcgacctt
      301 gatccgctac tgtggagttg cttttgcctg agaccgatcc ggcgctggcc gggcttcccc
      361 ctgacgccta cacacgggta tcggagcttg ccgatgaggt ggtacggatg tacggcggga
      421 ccgggccacc gttccctatt ctgcacctgc tggctatcct ggaacctgtc acgctgattc
      481 tgccgccgct tgccgatggg cgcgacgccg gggtctacgg ctatgccttt ccggtggagt
      541 tcgcgcgcta taagcggcac gccgcgctct gcctggactc aaacgcgccg acgcgggagg
      601 tattcgtcac cgcgttccac gaggcggggc atgtgctgct gcacatcctg ccggatcgct
      661 gcgcgccggg gattccgtat gcccgtcgcg ccgggccgcc gctggacccg cgccatgggc
      721 aggccgaaat cgaagccgac ctgttcgccc ggctgacgac catgcccgcc gcctgggtgc
      781 ggcccgcatg gctctatgcc gccgccgtga cggccacgcc ggacgaagcc gtcgcctgga
      841 tggccgacct gttcagcgtg cccgtaggat ggatgcagcg ccggctcctg cacctgggct
      901 tgctgtagcc tcggcgattg ccggaggtgg ctgaccacca ttgcataagg ccaggccgcc
      961 cgcttgcgaa cgcgtgggtc gattcgggtg gctccggacg tggcgacgca ggcaggaggg
     1021 attcgtagcg gcgcgattta tcgcgcctgc cgctttacga gcatctcgtt tggatcgcgg
     1081 ccctgccgtc ccggcatccg ggtaaagaga caggcgcgat gaatcgcgcc gctacgcctg
     1141 agaacttgct ggaatcggca ttagggccgt tgtgccacca ggatcaggcg gggagagtcg
     1201 ctggtgtagg ggtcgagatc gtaggtgccg tagatgccgg ccaggcggag gccggcgcgt
     1261 tcgagcagga gctgggcttc ccagcgatag gtgtagcgca gggtttgcgg gatggtggtg
     1321 cggcggacgc gcccggcggc gtcctcttcg tcgtagatcc agtggacgtg gtgttgctgt
     1381 tcggcgctgt cccacacgcc gctgacgaac ttgagcaccc gcccgccgcc gggccggtcc
     1441 caggcgccct gatgcaccag ttcgccgccg gcggggtagg ggccgcccgt gtccaccggg
     1501 ctgaggtcca gcaccaggag tccgccgcgc cgcagccgcg cctgcacatc gaccagcgtc
     1561 gccagttgat cggcctgcgt gggccggtag gcgaagctgt tcagggcgca gaggacgaga
     1621 tcgaagggac cgagatccgg gggcagggcc tccatgcggg cggcgaccag cgtggccggc
     1681 aggccggctg cggccaggcg agcacgggcc agggccagca tggcgggcga ggcatcgacc
     1741 ccggtgcaag tgtgcccggc ggcggccagg gccagcagca cccgcccgct gccgcaggcc
     1801 agttccagca ctcgcgccgc cggactccgc cgggggacgg cctgggccgc gcaaccccgg
     1861 tagaaggcca ggtcggcgtc gtaagcgccg tattcgagat cgtagaacgg cgcgaaccga
     1921 tccaggatgc ccgagtccgc cggggcatcc gggtcgtccg cggggcgggg gttaggcgcc
     1981 ggcatccagc tccaggggtg ggaccggccg gggccggtcg tgttcatcga gggccacgaa
     2041 gacgaagcgc ccggtgctgg tcagcgcgcg ctcgccgctg gtgacctcct cggcccagac
     2101 ctccacgtcg atctgcatcg acgtgcgccc caccgccacc agc
//
LOCUS       DASUYC010001134         2424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1201695, whole genome shotgun sequence.
ACCESSION   DASUYC010001134 DASUYC010000000
VERSION     DASUYC010001134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1201695"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(472..>2424)
                     /locus_tag="VF276_18515"
     CDS             complement(472..>2424)
                     /locus_tag="VF276_18515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608900.1"
                     /translation="PATAPPATGSTTTSRRRTRTPAPAAEPATPAPGTPALGTPLAAA
                     AAAPAAAVASAAAQAAPADATTTPTSTTTRRRRAASAASAAPPPAVAPESPAPAPPAP
                     PAATEATSTRPRTGTARRATKAAADTPTPLPMPAAPVPPAAPAAPPEAAVARAMPPAA
                     AAPASLEAPQARALEAGPMPVPAAGAAVVPPAPPPAAPPREAAGAPALQPVTRRNPVP
                     FIAVGAGVLLLLILAAVFGPGLLGARTGGAGSPTPAATAPPTAASLPSATSGLPVGAV
                     ASATPAQVAGGEPTLAPTEPATTAPTTAPPSPVPATAAPTAMEMPTPAAIPPTTSQGA
                     VGGLRFTDNGQHVVDQAVVTLTLDTLPAPPAGQGRVAGLVSSATGANLNLGRITPDAK
                     GAATLTFTDTQGANLLAGYDGFVVTNEALEGTPFAPSSDVVLHGQLPVQAMVHISHLL
                     VSYPDTPGQVGLEVGLRAQVNALVDHAQQLSGAQATGNLATVKQEAEAIANLIEGSKA
                     PNYGDLNKDGQITDPGDGFGFLQNGTHDGYLLGVKDHAELSAAAPDATDTVKQHAEGV
                     AITTDNVRDWVTQIRDKARAIIAAPNTTATRQLASDILDLATAVEQGRSIGTDGNIQP
                     VKGSGGAITSYQEALHMADMPIQPAK"
BASE COUNT          290 a          773 c          983 g          378 t
ORIGIN      
        1 accgccacgt agcggtccag cgactcctgg taggctttgt agaccgtggc catgccgccc
       61 cgccgaatga cttcggtgag ttggtactgg cccagttggc ttccgatcag gttacgcatc
      121 ggcctactcc gcgatggtca gcctggtttg cgtgcggtag cgttagagca ggagcttgcg
      181 gccaagccgg gtatgagcaa gtccctaggg gtacgtgcgg gatcaggcgg gaaggcggca
      241 acggcgagag cagatcaagc cttaagtaaa ctgcgaatag aaaccggctc cggcaagctg
      301 ttaatatgca attgatatta tactacgagg ataccctttg tcaataagct ggccgcccat
      361 ccggttacaa gcatcccgcg cagccacgaa ggacgcgaag atcggtgcca tgcttcgtgt
      421 gctgcgcggt gaacgctgag gcggtatgac cggcgccggc gcggggcggg actatttggc
      481 cggctggatc ggcatgtcgg ccatatgcag ggcctcctgg tagctggtga tggcgccgcc
      541 gctgcccttg accggctgga tattcccgtc ggtgccgatc gaccgcccct gctcgacggc
      601 ggtggcgagg tccagaatgt cgcttgccaa ctgccgggta gcggtcgtgt tgggggcggc
      661 gataatcgcc cgcgccttgt cgcggatctg cgtgacccag tcccgcacgt tgtcggtggt
      721 gatggccaca ccctcggcgt gctgcttgac agtgtcggtg gcgtccggtg cggcggcgga
      781 cagttcggcg tggtccttca cgcccagcag atagccgtcg tgcgtgccgt tctgcaggaa
      841 gccgaacccg tcgccgggat cggtgatctg cccgtctttg ttcagatcgc cgtaattggg
      901 cgccttgctg ccttcgatca ggttggcgat ggcctcggct tcctgtttca cggtggcgag
      961 gttgccggtg gcctgcgccc cgctcaattg ctgcgcgtga tcgaccagcg cattgacctg
     1021 cgcgcgcagg cccacttcca ggccaacctg gccgggtgta tcggggtagc tgaccagcag
     1081 gtgcgagata tggaccatgg cctgtaccgg caactggccg tgcagcacga cgtcgctcga
     1141 cggggcgaag ggcgtgcctt cgagcgcctc gttggtgacg acgaagccgt catagcccgc
     1201 cagcaggttg gcgccctggg tatcggtgaa ggtcaacgtc gcggcgccct tcgcgtcggg
     1261 cgtgatccgg cccaggttca ggttggcgcc ggtcgcgctg ctgacgagcc cggccacgcg
     1321 gccctggccc gcgggcgggg cgggcagggt gtccagggtc agggtgacga ccgcttgatc
     1381 gaccacgtgt tgcccgttgt cggtaaagcg caggccgccg accgcgccct ggctggtggt
     1441 gggcgggatc gccgcgggcg tcggcatctc catagcggtc ggcgcggcgg tggccgggac
     1501 cgggctgggc ggcgcggtgg tcggggccgt ggtcgccggc tcggtgggcg ccagggtcgg
     1561 ctcgccaccg gcgacctgcg ccggggtcgc cgaggccacg gcgccgacgg gcaggccgct
     1621 ggtcgcgctg ggcagggacg cggcggtggg cggggccgtc gcggcgggcg tggggctgcc
     1681 cgcgccgccc gttcgcgccc ccagcagccc cggcccgaag acagcggcca ggatcagcag
     1741 cagcagcacg cccgcgccca cggcgataaa gggcaccggg ttgcgccgcg tcaccggctg
     1801 caaggccggg gcaccggcgg cttcacgcgg cggggcggcc ggcggaggcg cgggcggcac
     1861 cacggcggca ccggcggcgg gcacgggcat cggcccggct tccaacgcgc gcgcctgtgg
     1921 tgcctctaaa gaggcaggcg cggcagccgc gggcggcatg gcgcgcgcca cggcggcttc
     1981 gggcggcgcc gcaggcgcgg cgggcggcac cggggccgcg ggcatgggca gcggcgtggg
     2041 cgtgtctgct gcggcttttg tggcgcggcg ggccgtgccg gtccgggggc gggtgctggt
     2101 cgcctcggtg gcggcgggcg gggccggcgg cgccggggcc ggagattcgg gggccacggc
     2161 gggcggcggt gcggcgctcg ccgcgcttgc ggcgcggcgg cggcgggtgg tggtcgatgt
     2221 aggcgtggta gtagcgtccg ctggggctgc ctgcgccgca gcactcgcta ccgcagccgc
     2281 gggcgcggcg gcagcggcgg ccaggggcgt gcccagcgcg ggcgtgcccg gcgcgggcgt
     2341 ggccggttcg gcggcaggcg ccggggtgcg tgtgcgccgc cggctggtgg tggtgctgcc
     2401 ggtcgcgggc ggcgcggtgg ccgg
//
LOCUS       DASUYC010001135         5256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_428413,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001135 DASUYC010000000
VERSION     DASUYC010001135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428413"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..4403)
                     /locus_tag="VF276_18520"
     CDS             complement(99..4403)
                     /locus_tag="VF276_18520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX6608901.1"
                     /translation="MTASRSTWLNLTLLRRLLVVPAAGLALYAERLAADARGAAPAPP
                     QLSWALFALAALVFVIAAWPPRALRESPGLRFGVARRALAQPLRFWAPLGLGLVCALL
                     AIPLFITINAAPPASPIGEPANTGAWLLWIAALLLFGAAWVVWERNTPAPPAGTLPAS
                     KEAPPADRLPVRIEWLVMGVLLLGALGLRVFSLDNVPPGLWFDEAQNGIVAQRLLAPD
                     APHPTFIGDLTQMGALYFYILGVVLKLAGTDIWPLRLLPALAGAVTVPMIYLIAARLY
                     GWRAGLAAAGVLMVSAWNITFSRFGMVSMTTVALDVAVYLCMVQALRTGRLGYYAGAG
                     VLLGLAMQGYYVSRLVPLVLLVLLVHQLISTRLRLLRAIRVGIVVFALGALLAFLPVG
                     LFAIQQPDSFSGRVSTVSVLNPEVNHGDANVVIDNLNKHLLMFNFRGDANGRHNLPGA
                     PMLDDLTAALFFLGLGACLLRAWRWQYAFPVVWFVAAISGGVLSIPFEAPQSHRTLEN
                     SVVTALLAGIVLGEFWQCLTRPRRVTEEADEAAPARKILTRALPWAGRVRPKAPATTL
                     PTRRRPVEAAGTLPVRTRGSAAETGAPQPAVLAAVAEPQPAHSPLADGENGTHPGETL
                     AAAGPGPAGPAEPATMALDTADQPLPSETPEALPDASQAPPEVLAPVPAAVRQRPSRN
                     RLLAWAVSAVGILLVVGAVSQVTIPRYFQIQARDRSVWGDMYSPEAQAARLVRSLSPN
                     YQVFVSSVFIGLPPMRYLVPDANPIEWPGMAALPLNGADRDVAIIIDPPQAADLTAIA
                     RMYPHASFDPFNAPSDPSPMLYSIRIPATDIAALHGAQVSLYDPGATRPREERTTPNF
                     ALDWAAVGKPGTARLATTLHVDQYGVYNFDWHPAGPAAEPALLVDGYPVRSGDPVALA
                     VGLHTVVATDTVGTATGTAQLGWGMQGNAAQPVPTANLFDPQRIEPHGLTGFYRQGTT
                     ASGPPQGVRVDSVISFNFHLLPMQMARPYNVEWRGRLYVPQAGPYALATEQISESHLF
                     VDGQEVIVNLRENNVVEAQLTLAAGWHDLRLLFIDAAGYSHMYLYWTPPGRGRSIIPS
                     AFLWPELGAYPTVPSTGAWPTLAESDASILPGQGTKAPPSLEAGGPTPPPVPVGAIPT
                     PPIAAAPAPTQAPAGPVTALTPSLLLGAQGPGLARPRAAAADAAGNIYLFTEQDSKIH
                     KFAPDGKEILAWDVRNSAGKPLTEGSALVVKDNQLRLLDAAASDLLSFDFDGKATGKT
                     HLCACFFPRGMAVSRDGNYWLADTGNNRLIKVTPDGQSVQTLGGKGTGPGQFAEPAGV
                     WETADGTLYVADIGNNRVQSLAPDGKPLAAWWVGPSIARDGNRLTGDSTGNVLVTIAA
                     QQAIARFNAQGQELQRWQFTNSGGILTPVGIAPAGADRFIALYLNNGLAAVFSAGR"
     gene            complement(4613..>5256)
                     /locus_tag="VF276_18525"
     CDS             complement(4613..>5256)
                     /locus_tag="VF276_18525"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEX6608902.1"
                     /translation="LDVFKQVLDELARMRYGISGYFHAEGEDGGRNYEYTVADDTWQV
                     NEARLRVKPIPADEGILLGQVYSQQDATANILRMLTGEQMVEPGVQGLWSIKDVLAHL
                     TEWQQRVSGWILVELQGETVGPDQIPPLAARDTLNEASYYVNRDRPLADVLAYYKAAN
                     LELIQLIARLPDASLYWHITENIVEHFQEHRAAIEAWIQRTYPALWPPARPNE"
BASE COUNT          838 a         1703 c         1915 g          800 t
ORIGIN      
        1 ccgacggcgc gccggcgccg cggatggcgc cgcgatctac cggcgcaaca acggcccggc
       61 actcgtggtg agcgccgggc cgccggcctg gtcgtccgtc accggcccgc actgaacacg
      121 gcggccagcc cgttgttcag gtacaacgcg ataaagcgat ccgctcccgc gggggcgata
      181 cccaccgggg tcaggatgcc gccgctattg gtgaactgcc agcgttgcaa ctcctggccc
      241 tgcgcgttga agcgggcgat ggcctgctgc gccgcgatgg tcacgagcac attgccggtg
      301 ctgtcgccgg tgaggcggtt gccgtcgcgg gcaatcgacg gccccaccca ccaggcggcc
      361 aggggcttgc cgtcgggcgc caggctctgg acgcggttgt tgccgatgtc ggccacatac
      421 agcgtgccgt cggcggtttc ccacacgccg gccggttcgg cgaactggcc gggccctgtg
      481 cccttgccgc ccagggtctg cacgctctgg ccgtccggcg tcactttgat caggcggttg
      541 ttgccggtgt cggccagcca gtagttgccg tcgcgggaca cggccatgcc gcgcgggaag
      601 aagcaggcgc acaggtgcgt cttgccggtg gccttgccgt cgaagtcgaa gctgagcaga
      661 tccgaggcgg cggcgtcgag caggcgcagt tggttatcct taacgaccag ggccgagcct
      721 tcggtcagcg gtttgccggc gctgttgcgg acatcccagg cgaggatctc cttgccgtcg
      781 ggcgcgaact tgtggatctt gctgtcctgt tcggtgaaga ggtagatatt gcccgcggcg
      841 tcggcggcgg cggcgcgggg ccgggccagt ccggggccct gcgcccccag cagcaggctg
      901 ggcgtcagcg ccgttaccgg cccggcgggc gcctgggtgg gggcgggcgc ggcggcaata
      961 ggcggcgtgg gaatggcccc caccggcacc ggcgggggag tcggcccgcc cgcctccagc
     1021 gacggtggag ctttcgtgcc ctggcccggc aggatactcg catcggactc ggccagcgtg
     1081 ggccaggcgc ccgtggacgg cacggtgggg taggccccca gttccggcca caggaaggcg
     1141 gacgggatga tgctgcgccc gcggcccggc ggggtccagt agagatacat gtgcgagtag
     1201 ccggcggcgt cgatgaacag caagcgcagg tcgtgccaac ccgcagccag cgtgagttgc
     1261 gcttcgacca cgttgttttc gcgcaggttg acgatcacct cctgcccatc gacgaacagg
     1321 tgggactcgc tgatctgctc ggtcgccagc gcatacgggc cggcctgcgg cacatagagc
     1381 cgcccgcgcc actcgacgtt gtacgggcgc gccatctgca tgggcaggag atggaagttg
     1441 aacgagatga cgctgtccac gcgcacgccc tgcggcgggc cgctggcggt cgtgccctgg
     1501 cgatagaagc ccgtgaggcc atgcggctcg atgcgctgcg gatcgaagag attggccgtg
     1561 ggcaccggct gggcggcgtt gccctgcatg ccccagccca gttgcgccgt gccggtcgcg
     1621 gtgccgacgg tgtccgtcgc cacgacggta tgcagcccaa cggccagggc caccgggtcg
     1681 ccggagcgga ccgggtagcc gtccaccagc agggccggtt ccgcggcagg gccggcaggg
     1741 tgccagtcga agttgtagac gccgtattga tcgacgtgca aggtcgttgc caggcgcgcg
     1801 gtgccgggtt tgcctaccgc cgcccaatcg agggcaaagt tgggcgttgt gcgctcttcg
     1861 cgcggacggg tggcgccggg atcgtagagg cttacctggg cgccgtgcag cgcggcgata
     1921 tcggtggccg ggatgcggat ggagtaaagc atcggcgacg ggtcggaggg cgcgttaaac
     1981 ggatcgaagc tcgcgtgcgg atacatgcgg gcgatggccg tgagatcggc ggcttgcggc
     2041 ggatcgataa tgatcgccac gtcgcgatcc gccccgttca ggggcagggc ggccatgccc
     2101 ggccattcga tggggttggc gtcgggcacc aggtagcgca tgggcggcag gccgatgaac
     2161 accgaggaca cgaacacctg gtagttggga ctcaggctgc gcactagacg cgcggcctgc
     2221 gcctcggggc tatacatatc cccccagaca ctgcggtcgc gcgcctggat ctggaagtag
     2281 cgcgggatcg tcacctggct aaccgcgccc acgacgagga ggatgcccac cgcgctcacg
     2341 gcccaggcga gcaggcggtt acggctgggc cgctgccgca cggcggcggg caccggcgcc
     2401 agcacctccg ggggcgcctg ggatgcatcc ggcagggcct ccggcgtttc ggacggtagg
     2461 ggttgatccg cggtgtccag cgccatggtg gcaggctccg caggccccgc cgggccggga
     2521 cccgccgccg ccagggtctc gccgggatgc gtgccgttct cgccgtccgc caggggtgag
     2581 tgggccggct gcggctcggc gacggcggcc agcacggcgg gttgcggcgc gccggtttcg
     2641 gctgcgctcc cgcgcgtgcg gacaggcaag gtgcccgcgg cctcgacggg acggcgccgg
     2701 gtgggcagcg tggtggccgg ggccttcggg cgcacccggc ccgcccaggg cagggcgcgg
     2761 gtcaggatct tgcgggccgg ggcggcctcg tccgcttcct ccgttacccg ccggggacga
     2821 gtgaggcact gccagaattc gccgagcacg atccccgcca gcagcgccgt gacgacgctg
     2881 ttttcgagcg tgcggtggct ttgcggcgct tcaaacggga tcgacagcac cccgccggag
     2941 atggcggcga cgaaccagac cacggggaag gcgtattgcc agcgccaggc gcgcagcagg
     3001 caggcgccca ggccgaggaa gaataacgcc gccgtcaggt cgtcgagcat gggcgcgccg
     3061 ggcagattat gccgcccatt ggcgtcgccg cggaagttga acatcagcag gtgcttgttc
     3121 aggttgtcga tcaccacgtt cgcgtcgccg tggttgactt cagggttcag gacggagacg
     3181 gtgctgacgc gcccgctaaa gctgtcgggc tgctggatgg caaacaggcc gaccggcagg
     3241 aaggccagca gcgcgccgag cgcgaacacc acgatgccca cgcggatggc gcgaagcagg
     3301 cgcaggcgcg tgctgatcaa ctgatgcacc agcaacacga gcagcaccag cggcaccagg
     3361 cggctcacgt agtaaccctg catcgccagg cccagcagca ccccggcccc ggcgtagtag
     3421 cccagccgcc cggtgcgcag cgcctgcacc atgcacagat agacggccac gtccagggcc
     3481 acggtggtca tggagaccat gccgaagcgg ctgaaggtga tattccaggc cgagaccatg
     3541 agcacacccg ccgccgccaa tcccgcgcgc cagccataga gccgcgcggc gatcaggtag
     3601 atcattggca ccgtgacggc gccggccagc gcgggcagca gccgcaaggg ccagatatcg
     3661 gtgccggcga gcttgagcac cacgcccagg atatagaaat agagcgcgcc catctgggtc
     3721 agatcgccga tgaaggtggg gtgcggggcg tcgggggcca gcaggcgctg ggcgacgatg
     3781 ccgttttgcg cctcgtcgaa ccagaggccg ggcggcacgt tgtccaggct gaacacccgc
     3841 aaccccagcg cgcccagcag caacacgccc atcaccagcc actcgatccg gaccgggagc
     3901 cggtcggcgg gcggtgcctc tttagaggca ggcagggtgc cggcgggggg cgcgggcgta
     3961 ttgcgctccc agaccaccca ggccgcgccg aacagcagca gcgccgcgat ccagagcagc
     4021 caggcgcccg tgttggccgg ttcgccgatg ggcgaagcgg gcggagcggc gttgatcgtg
     4081 atgaacagcg ggatcgccag cagcgcgcag accagcccca ggcccagcgg tgcccagaag
     4141 cgcagcggtt gggccagcgc gcgccgcgcc acaccgaacc gcaggccggg cgattcgcgt
     4201 agcgcccgtg gcggccaggc ggcgatcaca aacacgagcg cggccagggc aaacaaagcc
     4261 caactcagtt ggggtggggc cggcgcggcg ccccgtgcat cggcggcgag gcgctcggcg
     4321 tagagcgcca gccccgcggc gggcacaacc agcaggcggc ggagcagtgt gagattcagc
     4381 caggtggatc gagacgcggt catgggttcc gtgagcgggt gagcggccgg ggtcgccggg
     4441 gtacaaccgg ggatcgcagg cgcaggttgg acatcgctgg gattatagcc ggaccaacat
     4501 aagaggcagc tagccggggg taattgtatc acgttggccg ggccggcgtc aaccgcagtt
     4561 tagcgaatca ggcccttggc cggtggtttc acctgctgtt gggcctacct gattactcgt
     4621 tcggtcgcgc cggcggccac agggccgggt atgttcgctg tatccaggct tcgatggccg
     4681 cacgatgctc ctggaaatgc tccactatgt tttcggtgat atgccagtac aggctggcgt
     4741 cgggtagcct ggcgatcaat tgtattagtt ccaggttcgc cgccttgtaa taagccagta
     4801 catcggccaa tgggcgatcc cgattcacat agtatgaggc ttcgttaagg gtatcgcggg
     4861 ctgcgagagg tgggatctgg tcggggccga ccgtctcgcc ttgcagttcg actagtatcc
     4921 atcctgatac acgctgttgc cattcggtga gatgcgccag cacatctttg attgaccaga
     4981 gaccctgtac ccctggttcg accatttgct cgcctgtcag catccgcagg atgttggcgg
     5041 tggcgtcctg ctgactgtat acctggccca gcaagatccc ctcatcggcg ggaattggtt
     5101 taacgcgcaa gcgtgcctcg ttcacctgcc acgtatcgtc cgcgacggtg tactcgtagt
     5161 tccggcctcc atcttctcct tctgcgtgga aatatcccga tatgccgtag cgcatacgcg
     5221 ccaactcatc taatacctgc ttgaagacat caagcc
//
LOCUS       DASUYC010001136         3792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1350856, whole genome shotgun sequence.
ACCESSION   DASUYC010001136 DASUYC010000000
VERSION     DASUYC010001136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1350856"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VF276_18530"
     CDS             complement(<1..576)
                     /locus_tag="VF276_18530"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEX6608903.1"
                     /translation="MSTSPLAAEIARSAEALRAQLPADFRPRVLVILGSGLGALAGEV
                     DAVARIPYSEIPGWTASTVVGHAGRLICGTLEGVPLLMMQGRLHYYEGHSPAQATFPV
                     RVARALGAEMMIVTNAAGGLNPEYRPGDLMLIADHINMMGWGGANPLIGPNDESLGPR
                     FLPMNPAYDPALMDLATAEAAAQTRLLRRGVY"
     gene            complement(573..1367)
                     /gene="recO"
                     /locus_tag="VF276_18535"
     CDS             complement(573..1367)
                     /gene="recO"
                     /locus_tag="VF276_18535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX6608904.1"
                     /translation="MTTPLHERLYRTEAIVLRRSEIGEADYILTVYTPHLGKIRVVAR
                     GVRKPASHMGGHVELFSRSRMMIASARNLDIVTQCETLDPHRAIGEDLDRIAHAFYLV
                     EMVDRMTPDEQENYAVYKLLSESLTYLDTTPTPDILRHWFEIQLLGLLGYRPELTECV
                     ACRTTLEPVVNAWSPAQGGVLCPACKRSGVARDLSLTAFKVLRLMQRSPWPDVARVRV
                     PPQVAVELDQAMDTYVTYILEREPRSNLFLKVAEQTPDYIPQEGTP"
     gene            complement(1597..2220)
                     /locus_tag="VF276_18540"
     CDS             complement(1597..2220)
                     /locus_tag="VF276_18540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608905.1"
                     /translation="MPTRSNPRLRLLYTAILLLLLSGCGANPAGPAVPTPTALPATLP
                     PPGGIWLPTRTPAPPTPTPPPQPTSTRTPRPPTRTPAPPTVPAVFTPPAAGEVLIGTW
                     GSEDTHLTANRFGGAMAFQCAEGSLEQPLRVDAQGRFDVSGTYLDKPGFPRPTPPAPI
                     PARYQGQIIGSTMVLTLTLFYTKPYTSTYHLTFVSAEPTYIPPSPCA"
     gene            complement(2444..3238)
                     /gene="ehuA"
                     /locus_tag="VF276_18545"
     CDS             complement(2444..3238)
                     /gene="ehuA"
                     /locus_tag="VF276_18545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019928089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0033308 - hydroxyectoine transport
                     [Evidence IEA];
                     GO_process: GO:0051470 - ectoine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine/hydroxyectoine ABC transporter
                     ATP-binding protein EhuA"
                     /protein_id="HEX6608906.1"
                     /translation="MTIPTTPEAYSGEAIVQAEDVHKSFGHTEVLRGLNLTVHPGKTV
                     VLIGPSGSGKTTFLRCINHLEKIQRGRIYVGGQLIGYREQDGKLVEDSERNVAAQRAR
                     IGMVFQRFNLFPHMTALENVIEAPVHVKKLPKAEATEKARELLRKVGLADKADRFPNQ
                     LSGGQQQRVAIARALAMDPMLMLFDEATSALDPELVGEVLKVMEQLAAEGMSMVVVTH
                     EMGFARDVADRVVFMDGGVVCEEGPPAQIFEHPQQERTQNFLRRIL"
     gene            complement(3266..>3792)
                     /locus_tag="VF276_18550"
     CDS             complement(3266..>3792)
                     /locus_tag="VF276_18550"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX6608907.1"
                     /translation="LIIFATGLYQIGIRLDLLTSALLALSINEGAYMAEIIRAGIQSI
                     DPGQMEAAKSLGMTYGLGMRRIVLPQAARVILPPLGNEFNNMLKSTSLASVIAIQELL
                     SRGADLNHLYFKTLEIYTIISCYYLAMTTVVTWVQGWMESRLGDRRGVEAPAGFWGRL
                     RGNILSPRRAAPHR"
BASE COUNT          601 a         1174 c         1354 g          663 t
ORIGIN      
        1 gtagaccccg cggcgcagca ggcgcgtctg ggccgccgcc tcggcggtcg ccagatccat
       61 caaggccggg tcgtaggccg ggttcatcgg caggaagcgc gggcccaggc tctcgtcgtt
      121 cggcccgatc agcgggttgg cgccgcccca gcccatcatg ttgatatggt cggcgatcag
      181 catcaggtcg ccgggccggt attcggggtt gagaccgcct gccgcgttgg tgacgatcat
      241 catctcggcg ccgagggcac gggccacgcg caccgggaag gtggcctggg ccggcgaatg
      301 gccctcgtag tagtgcaggc gcccctgcat catcagcagc ggcacgcctt ccagggtgcc
      361 gcagatcagg cgcccggcgt ggcccacgac cgtgctcgcc gtccagccgg ggatctcgct
      421 gtaggggata cgggccacgg cgtccacttc gccggccagg gcgcccaggc ccgagccgag
      481 gatcacgagc acgcgcggac ggaaatcggc gggcagttgg gcgcgcagcg cctcggcgga
      541 acgggcgatc tcggcggcca gcggcgacgt gctcacgggg tgccctcctg cgggatgtag
      601 tcgggagtct gctcagccac tttcagaaaa aggttcgagc gcggctcccg ctccaggata
      661 taggtcacgt aggtgtccat ggcctggtcg agttcgaccg cgacctgcgg cggcacccgc
      721 acgcgggcca cgtcgggcca ggggctgcgc tgcatcagcc gcagcacttt gaacgcggtc
      781 agcgacaggt cgcgggccac cccggatcgc ttgcaggccg ggcagagcac gccgccctgc
      841 gccggactcc aggcgttgac caccggctcc agcgtcgtcc ggcaggcgac gcactcggtc
      901 agttccgggc ggtagccgag caggccgagc aactggatct cgaaccagtg gcgcaggatg
      961 tcgggtgtgg gcgtggtgtc caggtaggtg agcgactcgc tgagcagctt gtagacggcg
     1021 tagttttcct gctcgtcggg cgtcatgcgg tccaccatct cgaccaggta gaaggcgtgg
     1081 gcgatgcggt ccaggtcttc gccgatggcg cggtgcgggt cgagggtctc acactgggtg
     1141 acgatgtcga gattgcgcgc cgaggcgatc atcatgcggc tgcggctgaa gagttcgacg
     1201 tgcccgccca tatggctggc gggcttgcgc acgccgcgcg ccaccacccg gattttgccc
     1261 aggtgcggcg tgtagacggt gagaatatag tcggcctcgc cgatctccga acggcgcagc
     1321 acaatcgctt cggtgcggta taaccgttcg tgcagcgggg tagtcacagc agcatccctt
     1381 tcccggcccg cggcgcgggc aaccgagcca tcccgttcgg gtttgtgcgg agcgcacaaa
     1441 gtccctctct attatacccg aaaacggggc gcgacgcaca gaaagcaggc gttttgcata
     1501 tgtgctgggg ccgcagggcg gacatctgga tccgccccta ccatggacgc ggcggaccct
     1561 gtatctgcac ttgcattgta cgtctgccgc ttccccctac gcgcagggac tcggtgggat
     1621 gtaggtgggt tctgccgaga caaacgtgag atgataggtg ctggtatatg gctttgtgta
     1681 gaacagcgtc agtgtaagca ccatggttga gccgatgatc tggccctggt aacgtgcagg
     1741 gatcggcgcc gggggggtcg gccggggaaa tccgggctta tctaagtacg ttccggacac
     1801 atcaaagcgg ccctgggcgt ccacccgcag tggctgctcc aggctaccct cggcacactg
     1861 gaaggccatc gcgcccccaa agcggttggc cgtgaggtga gtgtcctcgc tgccccaggt
     1921 gccgatgagc acctcgccgg cagcgggagg ggtgaagacg gcgggcacgg tgggtggcgc
     1981 cggggtgcgg gtggggggcc ggggcgtgcg ggtggatgtg ggctgcgggg ggggtgtcgg
     2041 cgtaggcggc gcgggggtcc gtgtagggag ccagatgccg cctgggggcg ggagagtggc
     2101 agggagggcc gtgggcgtgg ggaccgccgg gccggcggga ttggccccgc agccactcag
     2161 cagcagcagc aggatggcgg tgtatagcaa gcgcaagcgt gggttgcttc gggtcggcat
     2221 cgtttcctcc ggcgtcgttg gtacgtggcc ccgcgtatac acacaactcc gttcgtagca
     2281 gcattgtagc acaccaccga gctcatcagc actaccttga ccctagtact ttcatcttat
     2341 attagtccta gtttctggca caaaacccga atgccggtcc tagatcgcgg ggcatgaaca
     2401 gctacgaccg cccgacgggg cggcctctac ccgtgcccgg cagctagagg atgcggcgca
     2461 ggaagttttg cgtgcgctct tgctgggggt gctcgaagat ctgcgccggg gggccttctt
     2521 cgcagaccac gccgccatcc atgaagacca cgcggtcggc cacgtcgcgg gcaaagccca
     2581 tctcgtgcgt gacgacgacc atgctcatgc cctcggcggc cagttgctcc atcaccttga
     2641 gcacctcgcc caccagttcg gggtcgagcg cggaggtggc ctcgtcaaag agcataagca
     2701 tcgggtccat cgccagggcg cgggcaatgg cgacgcgctg ctgctgcccg ccggagagct
     2761 ggttggggaa ccgatctgcc ttgtcggcca ggcccacttt gcgcagcaac tcgcgggctt
     2821 tctcggtcgc ctcggccttg ggcagcttct tgacgtggac cggcgcctcg atgacgtttt
     2881 cgagcgccgt catgtgcggg aagaggttga agcgctggaa gaccatgccg atgcgcgccc
     2941 gttgcgcggc cacgttgcgc tcgctgtctt ctaccagctt gccgtcctgt tcgcggtagc
     3001 cgatcaactg gccgccgaca tagatccgcc cgcgctggat cttctccagg tgattgatgc
     3061 agcgcaggaa agtcgtcttg cccgaaccgc tcggcccgat cagcaccacg gtcttgccgg
     3121 ggtgcacggt gaggttgagg ccgcgcagca cctcggtgtg cccgaagctc ttatgcacat
     3181 cctcggcctg gacgatggcc tcgccgctgt atgcctcggg ggtggtgggg atagtcatgg
     3241 atctttcctc ctgtgcgggc ggaatctagc gatgcggggc ggcgcggcgg gggctgagga
     3301 tgttgccgcg caggcgtccc cagaacccgg cgggcgcttc cacgccgcgg cggtcgccca
     3361 ggcggctctc catccacccc tgcacccagg tgaccaccgt ggtcatggcg agatagtaac
     3421 aggagatgat ggtgtagatc tccagggtct tgaaatagag atggttgagg tcggccccgc
     3481 gggacagcaa ctcctggatg gcgataaccg aggccagcga cgtgctcttg agcatgttgt
     3541 tgaactcgtt gcccagtggc ggcaggatca cgcgcgcggc ctgcggcagc acgatgcgtc
     3601 gcatgcccag gccgtaggtc atgccgagcg acttcgcggc ttccatctgg ccggggtcga
     3661 tgctctggat gccggcgcgg atgatctcgg ccatatacgc cccctcgttg atgctcaggg
     3721 ccagcagcgc cgaggtgagc agatccaggc ggatgccgat ctgatagagg ccggtggcga
     3781 agataatgag ct
//
LOCUS       DASUYC010001137         2420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_429824,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001137 DASUYC010000000
VERSION     DASUYC010001137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429824"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..944)
                     /locus_tag="VF276_18555"
     CDS             complement(108..944)
                     /locus_tag="VF276_18555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608908.1"
                     /translation="MNIVCFVRPAPGWDKFRFNLSTGAVEVPRDVPLGVSAFGEGTWV
                     LHPADRAALDAAATLLAATGADGTLTVVALESEVPDAAATVLREALARGAARAVLVTG
                     APDADSYVTASALAAVIGQAPDDGKPDLVVLGADAPGGAPDEVGPMLAEDLGWAQITA
                     ALTLAPAPDDPAHLRADQQWVAGLRTVDAALPLVVSLLPNAALTPRYARGANVLAAYR
                     KGEIESVDVAALGLTGANAAQGVHRVVVRRAALGEAPAAERLTGPVEESVAVLLPALR
                     QS"
     gene            complement(941..2101)
                     /locus_tag="VF276_18560"
     CDS             complement(941..2101)
                     /locus_tag="VF276_18560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6608909.1"
                     /translation="MDYTLPPEYRMLQRTIREFVDAEIAPIAAQIDREDCTPPSLVRK
                     MAQAGMFGLPFSTKYGGGGAGELGYCIMMEEIARASSAVGVIVGAHIGIGGGAIYIDG
                     SEEQKQRYMPALCRGEKLAAFCLTEPQAGSDAANLRTTATRDGDYWVLNGTKLWASNG
                     NRADILSVFAANDRSMGARGGITAFIVEKEFGGVTVARLEEKMGLHGSDTALLIFENC
                     RVPRENVIGMVGAGFLTAMKTLDGGRLSLGAAALGGAQYGLETMVRWAQAAVENGQPL
                     ANRQAIQWMIADTAMEVAALRHMVYSAAWLFDQGKKVSRESAMIKAYATEVGARTVDR
                     AVQVHGRAGLRHGAPVERHFRDERIARIFEGTNEVQRMIIAQETYRQMGYRA"
     gene            complement(2118..>2420)
                     /locus_tag="VF276_18565"
     CDS             complement(2118..>2420)
                     /locus_tag="VF276_18565"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX6608910.1"
                     /translation="LEIMLMGTDISAQEALRMGLVNKVVPEGTVVREARNMARVLATK
                     PSGAVQAILSMVQEGYGKPQSEALAMERDRFSQLVGTPDMREGLSAFIEKRKPAFQ"
BASE COUNT          337 a          875 c          836 g          372 t
ORIGIN      
        1 gtatagccgt cagcagtcag ctgtcagccg tcaggtgacg ataaacgata ctcgctactg
       61 aggatcagca ctaaaataac tcgtactcag cactcagcac tcagtactta ggactgccgc
      121 agcgctggca gcaagacggc cacgctctcc tcgaccgggc cggtcaggcg ctcggcggca
      181 ggggcctcgc ccagggcggc ccggcggacg acgacgcggt ggacgccttg cgcggcgttg
      241 gcgccggtga ggcccagcgc ggccacgtcc acgctctcga tctcgccctt gcgataggcc
      301 gccaacacat tggcgccccg cgcgtaacgc ggcgtcaggg cggcgttggg cagcagcgag
      361 accaccagcg gcaaggcggc atcgacggtg cgcagcccgg ccacccactg ctggtcggcg
      421 cgcaggtggg ccgggtcatc aggcgccggg gccagggtca gcgcggcagt gatctgcgcc
      481 cagcccaggt cttcggccag catcgggccg acctcgtcgg gcgcgccgcc cggcgcatcc
      541 gcgcccagca ctaccagatc gggcttgccg tcatccggcg cctggccgat cacggcggcc
      601 agcgcgctgg ccgtcacgta ggagtcggcg tcgggcgcgc ccgtgaccag cacggcgcgg
      661 gcggcgccgc gggccagcgc ctcgcgcagc acggtcgcgg cggcgtcggg cacctcggac
      721 tccagggcca ccacggtcag cgtaccgtcc gccccggtcg cggcgagcaa ggtcgcggcg
      781 gcgtcgagcg cggcgcggtc ggcgggatgc agcacccagg tgccttcgcc gaaggccgac
      841 acgcccagcg gcacgtcgcg cggcacctcg acggcgccgg tgctcaggtt gaagcggaac
      901 ttgtcccagc cgggagccgg gcggacgaag cagacgatgt tcatgcgcgg taccccatct
      961 ggcgatacgt ctcctgggcg atgatcatgc gctgcacctc gttggtgccc tcgaagatgc
     1021 gggcgatgcg ctcgtcgcgg aagtggcgct ccaccggggc gccatggcgc aagcccgccc
     1081 tgccgtggac ctggacggcc cggtccaccg tgcgcgcccc cacttcggtc gcgtaggctt
     1141 tgatcatggc cgactcgcgg ctcaccttct tgccctggtc gaacagccag gccgcgctgt
     1201 agaccatgtg ccgcagcgcg gcgacttcca tggccgtatc ggcgatcatc cactggatgg
     1261 cctggcggtt ggctagcggc tggccgttct cgaccgccgc ctgcgcccag cgcaccatcg
     1321 tctccagccc gtactgcgcg ccgccgagtg ctgccgcgcc caggctgagc cgcccgccgt
     1381 cgagcgtctt catcgcggtc aggaacccgg cgccgaccat gccgatgacg ttctcgcgcg
     1441 gcacgcggca gttctcgaag atcagcaggg ccgtgtccga gccgtgcagg cccatcttct
     1501 cctccagccg ggccacggtg acgccgccga actccttctc gacgatgaag gccgtgatgc
     1561 cgccgcgcgc ccccatgctg cggtcgttgg cggcgaagac gctgaggatg tcggcgcggt
     1621 tgccgttgga ggcccagagc ttggtgccgt tcagcaccca gtagtcgccg tcgcgggtgg
     1681 cggtggtgcg caggttggcg gcgtcggacc cggcctgcgg ctcggtcagg cagaaggcgg
     1741 ccagcttttc gccccggcag agcgcgggca tgtagcgctg cttctgctcc tcgctgccgt
     1801 cgatgtagat ggcgccgccg ccgatgccga tgtgcgcgcc gacgatcacg cccacggcgg
     1861 acgaggcgcg ggcgatctct tccatcatga tacagtagcc cagttcgccc gcgccgcccc
     1921 cgccgtactt ggtggagaag ggcagcccga acatcccggc ctgggccatc ttgcgcacca
     1981 gcgagggcgg cgtgcagtcc tcccggtcga tctgcgcggc gatgggcgcg atctcggcgt
     2041 ccacgaactc gcgaatggtg cgctggagca tccggtactc cggcggcagc gtgtagtcca
     2101 tgcttgctcc gtcctgtcta ctggaacgcg ggcttgcgct tctcgataaa cgcgctcaag
     2161 ccctcgcgca tgtcgggcgt gccgaccagc tggctgaagc ggtcgcgctc catcgccagc
     2221 gcctcgctct ggggcttgcc gtagccctcc tggaccatgc tcaggatggc ctggaccgcg
     2281 cccgacggct tggtcgccag cacgcgggcc atgttgcgcg cctcgcgcac caccgtgccc
     2341 tccggcacga ccttgttcac caggcccatg cgcaaggctt cctgcgcact gatgtcggtg
     2401 cccataagca tgatttcgag
//
LOCUS       DASUYC010001138         2031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_893515,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001138 DASUYC010000000
VERSION     DASUYC010001138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893515"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1225)
                     /locus_tag="VF276_18570"
     CDS             complement(<1..1225)
                     /locus_tag="VF276_18570"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HEX6608911.1"
                     /translation="MTIRTPVGALAGRREPGRPMEEAEAPRWPFTADTMIPLNRLTLR
                     QKVGQLITVEIPDARLTPKIAHFIRHCHPGGIAIFGRNLAGPWATAQLISDLQGVAAA
                     AGDAPLLIGIDQEGGQVSRLRYPAAELPSNMACNAAGGPAAAGEAAEILGAEMAAMGI
                     NLAYAPVADINTAATNPVIGTRAYSDDPRQVAACVREAITGLRRGGVFSMAKHFPGHG
                     DTLADSHLKLPSVSAGWDRMESVELVPFRAAIAAGVDTICSAHVRFPGIDPSHLPATL
                     SHRLMTDLLRKELGFQGVLFADALVMDAIAGRKGGNIPPAAVAAINAGVDCVMILGSL
                     RLQAQVYDTLLGAVQAGVISEERLDEAVARVYALRCRVTEPPAMPAWPVEAHRRAADR
                     LAHAAVTLVRDRDHLL"
     gene            complement(1271..>2031)
                     /locus_tag="VF276_18575"
     CDS             complement(1271..>2031)
                     /locus_tag="VF276_18575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608912.1"
                     /translation="PPAECILNDGGLPVTVDPGNPAYRARLTEEVRAMLQDLDADGFK
                     IDFTHLVPRGPRTATQPVPSSDEPARAAVAAQEVLAVAGHILWTRTPLGAAANGHWGL
                     ELMRDWLSIVSDAARAAKPDALIITHTANPYLADLADMLRLNDIAGLDDIEGSIVPDM
                     RHRTRIARAASPGWLIDSDNWPCLTRTQWRDYVLAQGSGEFGVPSLYFAERLGWGPTD
                     EPLDEEDYAAVRAAWAAYRARIPATAGLPPQSPA"
BASE COUNT          279 a          668 c          791 g          293 t
ORIGIN      
        1 gcagcaggtg gtcgcggtcg cggacaagcg tgaccgcggc gtgggccagc cggtcggcgg
       61 cgcggcggtg ggcttccacc ggccacgcgg gcatggccgg cggctccgtg acccggcaac
      121 gtaaggcata gacgcgcgcc acggcctcgt ccagccgctc ctcggagatg acgcccgcct
      181 gcacggcacc gagcagggtg tcgtagacct gggcctgcag gcgcaggctg cccaggatca
      241 tcacgcagtc caccccggcg ttgatcgcgg ccacggcggc gggcgggata ttgccgccct
      301 tgcgcccggc gatagcgtcc atgaccaggg cgtcggcgaa gagcaccccc tggaaaccca
      361 gctccttgcg cagcagatcg gtcatcagcc ggtgcgagag cgtggcgggc aggtgcgagg
      421 ggtcaatgcc ggggaagcgc acatgggccg agcagatcgt atcgacgccc gcggcgatgg
      481 cggcgcggaa cggcaccagt tcgaccgact ccatgcggtc ccacccggcg gacacgctgg
      541 gcagcttgag gtgcgagtcg gccagggtat cgccgtggcc ggggaagtgt ttcgccatgc
      601 tgaacacgcc gccccggcgc agaccggtaa tcgcctcgcg cacgcaggcg gccacctgcc
      661 gggggtcgtc ggagtaggcg cgcgtgccga taaccgggtt ggtggcggcg gtgttgatgt
      721 cggcgacggg cgcgtaggcg agattgatgc ccatggccgc catctcggcg cccaggatct
      781 cggcggcttc gcccgcggct gccgggccgc ccgcggcgtt gcaggccatg ttgctcggca
      841 attcggcggc cgggtagcgc agccgggaga cctgcccgcc ctcctggtcg atgccgatca
      901 gcaggggggc gtccccggcg gcggcggcga cgccctgcaa atcggagatg agctgggcgg
      961 tggcccaggg gccggccagg ttgcggccaa agatggcgat cccgccgggg tggcagtggc
     1021 ggataaagtg cgcgattttg ggggtcagcc gggcgtcggg gatctccacg gtgatgagct
     1081 ggccgacttt ctggcgcagc gtcagccggt tgagcgggat catggtgtcc gcggtaaacg
     1141 gccagcgcgg cgcttcggct tcctccatcg ggcggcccgg ctcgcggcga ccggctagcg
     1201 caccgacggg tgttctgata gtcattacgt tccttccaaa cgtgatgagg cgcgcccgtg
     1261 ggcgggccga ttaagccggg gactgcggcg gcaggccggc ggttgcaggg atgcgggcac
     1321 ggtaggcggc ccaggccgcg cgcaccgccg cgtaatcttc ctcgtccagg ggttcgtcgg
     1381 tcggacccca gccgaggcgc tcggcgaagt agagcgaggg cacgccgaac tcgccgctgc
     1441 cctgggccaa cacgtagtcg cgccactgcg tgcgggtcag gcagggccag ttatcggagt
     1501 cgatcagcca gccgggcgag gcggcgcgcg cgatgcgcgt gcggtggcgc atgtcgggca
     1561 cgatactgcc ctcgatgtcg tcgagcccgg cgatgtcgtt gaggcgcaac atatcggcca
     1621 ggtcggccag gtaggggttg gccgtgtggg tgatgatcag ggcgtcgggc ttggcggcgc
     1681 gcgccgcgtc cgagacgata ctcagccagt cgcgcatcag ttccaggccc cagtggccgt
     1741 tggccgccgc gcccagcggc gtgcgcgtcc agaggatgtg cccggcgacg gccagcactt
     1801 cctgagcggc gacggcggcc ctggccggtt cgtcgctgct gggcaccggc tgggtggccg
     1861 tgcgcggccc gcgcggcacc aggtgggtga agtcgatctt gaagccgtcg gcgtcgaggt
     1921 cctgcaacat ggcccgcact tcctcggtca gccgcgcccg gtaggccggg ttgcccggat
     1981 cgaccgtcac cggcaggccg ccgtcgttca ggatgcactc cgccgggggc a
//
LOCUS       DASUYC010001139         9322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1265288, whole genome shotgun sequence.
ACCESSION   DASUYC010001139 DASUYC010000000
VERSION     DASUYC010001139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 9322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9322
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1265288"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(371..829)
                     /locus_tag="VF276_18580"
     CDS             complement(371..829)
                     /locus_tag="VF276_18580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608913.1"
                     /translation="MTTRTDAHMIAGELARGLNQLCALGLLSEGGRGLRPAARLEVVR
                     DALAQTESGALAGYGSTDTRQALDVWRLVQRMFESNQTPFSDMDNQELAQFLEKLRKA
                     AYGPQISHAMARWLPSRMFISAQYTTQQLEKMRPAGSAGRGLSSRPAGLR"
     gene            complement(1104..2957)
                     /locus_tag="VF276_18585"
     CDS             complement(1104..2957)
                     /locus_tag="VF276_18585"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6608914.1"
                     /translation="MRVEKELVVNAPVSTVYALWTDFENFPRFMSHVESVTKVSDDTY
                     HWKAKVGLRNREWDARVVGLVPNRSVTWRSVEGEENAGAVNLAEQGNVTLMQVVIEYH
                     PNWFEGVLDTVTHEMSRSVEADLEKFKRLAEGTEGEAAQMQTGAQAQAGDTGGAQGAT
                     SESVGAGNAGTVSPSSMRAGQRGSGVTSGISASGAASMASAAAATEATRSHDAAPMSR
                     SGAMQGGSEGGGEYASGGASSGSLGGSSRSGSMGQETPVPGEETIIGSMGPGGANASL
                     PSQAGDIGADSRDLSVHSGEAQHGFGVPPVDETAGGGPPMPVSGSHHNYAGTDIVPEP
                     GATGFSDTNAGSAGGDTGSGATADNATGVAMDSGAAAGLDSTSGGGSQQGAVRNTPAG
                     ANQPDNATGGSGLTGAGAPTGTASIAGTDTAGVTGPSLYSNEAAGYEGYGMTPGETPA
                     TIGGTAAASGGESNTAGSGASSVDRGSSAAAQTGGTTGSTPGDTTGGSGMSGRAAGAG
                     TPTQTTGAISGSMQDESVRPIDEERGTTPGMVGGTGGWGSPTGGAPGSGAMGDLEQGK
                     GVGGAATGDPGQEVGLPAVSTTDMNLGQQPTGESMSMGPSTASGGTGSLGS"
     gene            complement(3121..3651)
                     /locus_tag="VF276_18590"
     CDS             complement(3121..3651)
                     /locus_tag="VF276_18590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016863082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6608915.1"
                     /translation="MPEFETPRLRLRLFRRDDLDALAAIFSKPAVMRFMPGGQPIPRE
                     RIPAGIERRIQDWEMRGFGLFALEDKATGALIGQAGLFPLEDTDEVEVAYLIDEPYWG
                     QGLATEAARACLRFGFEHLHLPRIVAVAVPANRASTRVMEKLGMEYQGPAHHYGLDVA
                     RYAITRESFATLNTDH"
     gene            complement(3865..4557)
                     /locus_tag="VF276_18595"
     CDS             complement(3865..4557)
                     /locus_tag="VF276_18595"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX6608916.1"
                     /translation="MNIQAIIFDMDGVLIDSEPLHHAAMNAVLAPLQQRVDDPEYRTY
                     IGASSAYAWERIVAARGLPDPPASYMTRYDEAVCAVVAEQGVPMPGLLPLLAALRAAG
                     VPLAVASSSAARWVQVTLGCIGVRDYFAVVVSGEQVTRGKPAPDIFLRAASLLGLDPA
                     QCLVIEDSARGLEAARAAGMFAVALQAAPLPPGAPAPAADLVVASLPEFHAWWRASGQ
                     RLAVSHQPSGDE"
     gene            complement(4554..5255)
                     /locus_tag="VF276_18600"
     CDS             complement(4554..5255)
                     /locus_tag="VF276_18600"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050532 - 2-phosphosulfolactate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HEX6608917.1"
                     /translation="MQIHFATLDTCAEAAGTVVVIDVIRAMTTAAYAFAAGVRDIVPV
                     GTVEEALALRARFPGALVMGEVGGLPVPGFDFGNSPAALIGRDLHGTRLIQRTSAGTQ
                     GLVRSARASLLLGGNFVCAAATVRHIRAQGAGEVTLVTTGQEDEDQACAEYLAALLRG
                     ERPPVAALLARTHDAGLMRMSDAGFGHDFTEAERVEFVADLCCCTALDRFDFVLRVER
                     QADLLVMAPVPARPR"
     gene            complement(5263..5991)
                     /locus_tag="VF276_18605"
     CDS             complement(5263..5991)
                     /locus_tag="VF276_18605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEX6608918.1"
                     /translation="MDLHINFLYPDLMNIYGDRGNIIAMQRRAEWRGITVHVHTTTIG
                     ESLDITRDDFYFFGGGQDRQQISVAQDLAGTKGAQLREAVENGAALLSVCGGYELLQH
                     YYRPADGQELPGTGLFDAYSVAGDTRFIGNVVVETDFVGELVGFENHSGLTFLNPGCR
                     PLGRNIVGYGNNGRDGQEGAIYKTAYGCWIHGSLLPKNPRFTDYLLRQALHRRHGDVD
                     LAPLDETVEQAAHATAVRRAHATR"
     gene            complement(6019..7050)
                     /locus_tag="VF276_18610"
     CDS             complement(6019..7050)
                     /locus_tag="VF276_18610"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608919.1"
                     /translation="MKDQIVVRAPVRVDFAGGPSDVAPFCTEEWGYVVNAAIDKYAVV
                     RVARRTDGQVVLASADLGIQETYPSVAALRHDTPLRLLTTAIELTGIRQGVEVQVQTD
                     VPAGSGLGASAAVSVALLYALHEISGEGVPAGADLAQAAIRHETGKLHNVGGGQDQYA
                     ATFGAVQSYRFAAGEVGRRYLAVAPETVQALNESLLLCYSGTSRISGAVLDGVMRRYV
                     AGDAAVTGHLRRLREIAAEIEAMLEAGDIRGLGPHMTAAWHAFRALHPSVSTPRIEQV
                     LAVAAAHGATGGKATGAGGGGCVLLACAPGTRDAVAAALHEAGFSTIPFHITRQGVHI
                     VTERPPGQE"
     gene            complement(7066..8487)
                     /locus_tag="VF276_18615"
     CDS             complement(7066..8487)
                     /locus_tag="VF276_18615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HEX6608920.1"
                     /translation="MVKGIGPREMAAIAAAQGAGQAARLLRRGGGTALPGLVAERVSP
                     RILRRLTRQLRRGVIIVTGTNGKTTTSRMLGAILDAAGWHAIHNRSGSNLVRGLIATL
                     VQESNLGGNLHADSGLFEVDEATVPAVVAQVRPRLLLINNLFRDQLDRYGEIDHIHRR
                     WRETLATLDARSTVVLNADDPAVAVLGRSLAARVCYYGIADTGQALAALPHAADAIDC
                     PVCGHRLDYSAILLSHLGHYRCPACGFVRPAPDIVAESVALQGTEATRLTVRAGEQTF
                     PVELAVPGFYNAYNGLGACAAALTLGLSADIIRRGLGGFRAAFGRIERVAAGTSGKHL
                     LLALVKNPVGFNEVLRMLFPPQHEAPPRHLLIIINDLIADGRDVSWLWDVDFEVLGEP
                     GRVASATCAGIRATDMANRLKYAGLDPARIRVVEELDAALEATLAALPAGETLYVLPT
                     YTAMLDFRALLQRRGWVSQFWEQ"
     gene            complement(8481..9206)
                     /locus_tag="VF276_18620"
     CDS             complement(8481..9206)
                     /locus_tag="VF276_18620"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HEX6608921.1"
                     /translation="MARTTQLFELQQVDSQSDELLRRALAIKHALDETDSLKLARERH
                     AEAATALAVAQERLRVLSHEVDEMTAHLTLQSKRLYDGSVKNPKELVAIQHDITATKE
                     VRSAREDVMLEAMDAVDQAQAAVDAAAQDLEVAETEWQRNQEGLLEEKDAIDGQLRVL
                     RTRREKMVAGIAWADLQLYEKLRKAKHGTAIAGLEHDVCQACRVTVPAGTLRTVKQGL
                     EFVYCPSCARVLHPGARGDLVRW"
BASE COUNT         1329 a         3362 c         3200 g         1431 t
ORIGIN      
        1 cccccccccc cccccaccgc cgcggctgcg ggcagcggcg cgcccagggc cgcctcgcgt
       61 cccccggcgg cggcgaacac ggccaggacc agcaggacag ccgcgaggag catcccgccc
      121 cacagcatgc cgcgattacg catcttcagc tccttccaga caggagggtg gggatgagac
      181 cacccgagag ggaacaacga cggcacaggt tgcgttgcgc ggtggcctca gtggaggcac
      241 ggatggcgcc gggcggcgcc aattgcaact agctagagta taccaaatca aacgagcggg
      301 cgggagggca aacggcgacg gccccgctct gccggagccg ggccgcaacg cacgggagga
      361 acgccggagg ctaacgcagg ccggcgggcc ggctgctcag gccgcgcccg gcgctgcccg
      421 cggggcgcat cttttcgagt tgctgcgtgg tgtactgggc gctgataaac atgcgcgagg
      481 gcagccagcg cgccatggcg tgcgaaatct gcgggccata ggccgccttg cgcagcttct
      541 ccagaaactg cgccagttcc tggttatcca tgtccgaaaa cggggtctgg ttgctctcga
      601 acatccgctg gacgaggcgc cacacgtcga gcgcctgccg cgtatcggtg gagccgtacc
      661 cggcgagcgc gccgctctcc gtttgcgcga gcgcatcgcg caccacttcc aaccgcgccg
      721 cgggccgcag gccgcgcccg ccttccgaaa gcaagcccaa cgcgcaaagc tggttcaggc
      781 cgcgcgccag ctcgcccgcg atcatatggg catccgtccg ggttgtcatc cgccgttcct
      841 tcctgtgctg cgagtgatct acaaataccc cgcgcagcca gccggcgtgg ggggtccgct
      901 cttaagtgta gcagaccgcg acaaagcagg caatagccta ttggtaccaa gtatatccta
      961 gaaatcggct atcgtaatgc ggcgttgtag gggccggtcg caacccggcc cgccgcccgg
     1021 cggactcatg gtgagaacga tgcggcgggg gtggctccca cacggaagcc acccccgcgg
     1081 ctaaagtgtg ctacttgaca gcgttaactc ccgagcgagc cggtcccgcc gctggcggtg
     1141 gacggcccca tcgacatcga ttcgccggtg ggctgctggc ccaggttcat gtcggtcgtg
     1201 gagacggcgg gtagcccgac ctcctggccc ggatcgccgg tggccgcgcc gccgacgccc
     1261 ttgccctgct ccaggtcgcc catggcgccg ctgccggggg cgccgccggt ggggctgccc
     1321 cacccgccgg tgccgccgac catgcccggc gtggtgcccc gctcctcgtc aatcgggcgc
     1381 accgactcgt cctgcatgct gccggagatg gccccggtcg tttgggtggg ggtgcccgcg
     1441 ccggcggcgc ggccggacat gccgctgccg ccggtcgtgt cgccgggcgt gctgccggtg
     1501 gtgccgccgg tctgcgcggc ggcgctgctg ccgcggtcca cggaactggc gccgctgccg
     1561 gccgtattcg actcgccccc gctcgccgcc gcggtgccac cgatggtggc gggtgtctcg
     1621 ccgggggtca tcccgtagcc ctcgtagccg gcggcttcgt tggagtagag cgacgggccg
     1681 gtcacgcccg ccgtatccgt gcctgcgatg ctcgcggtgc cggtgggcgc gcccgccccg
     1741 gtcagaccgc tgcccccggt ggcgttatcg ggctggttgg ccccggcggg cgtgttgcga
     1801 acggcgccct gctggctacc cccaccagag gtggagtcca gcccggcggc ggccccgctg
     1861 tccatcgcca cgccggtcgc attgtcggcg gtggccccgc tcccggtatc gccgcccgcc
     1921 gacccggcgt tggtgtcgga gaacccggtg gcgcccggtt ccggcacgat gtccgtgccg
     1981 gcgtagttgt gatgcgaccc ggagaccggc atgggcggcc cgcctccggc ggtttcgtcc
     2041 accggcggca cgccgaagcc gtgctgggcc tcgccgctgt ggacactcag gtcgcgcgaa
     2101 tcggcgccga tatccccggc ctgcgacggc aaactggcgt tggcaccgcc cggccccatc
     2161 gagccgatga tcgtctcttc gcccggcacc ggggtttcct ggcccatgct gccgctgcgc
     2221 gaggagccgc cgagcgaccc gctgcttgcg ccgccgctgg catattcgcc gccgccttcc
     2281 gagccgccct gcatcgcgcc cgagcgcgac atgggcgcgg catcgtggga gcgggtcgcc
     2341 tcggtggccg ccgccgccga ggccatgctc gcggcacccg atgcgcttat gccgctcgtg
     2401 accccgctgc cgcgctgccc ggcgcgcatg gaactgggac tgaccgtgcc cgcattcccg
     2461 gcgccgacac tctcgctggt cgcgccttgc gcgccaccgg tgtcgccggc ctgcgcctgg
     2521 gcgccggtct gcatctgggc cgcctcgccc tccgtgcctt cggccagtcg cttgaatttc
     2581 tccaggtcag cctccacgct gcgcgacatt tcgtgcgtca ccgtgtcgag cacgccctca
     2641 aaccagttcg gatgatactc gatcaccacc tgcatcaggg tcacgttgcc ctgctcggcc
     2701 agattcacgg cgccggcgtt ttcctcgcct tcgaccgagc gccacgtcac cgagcggttg
     2761 ggcaccaggc ccacaaccct ggcatcccat tcgcggttgc gcaggcccac cttggccttc
     2821 caatgatagg tatcgtcgct caccttcgtg accgactcta catggctcat gaagcgtgga
     2881 aagttttcaa aatcggtcca cagggcatat acggtactga ctggggcatt gaccaccagt
     2941 tctttctcaa cccgcatttg gaacctcctc gttcagttcg gcaggtgccg tctccggcca
     3001 gtaccatccg cggaaggtcg cccaccggcg gccacgtccg cgcccctggc ctgatagagc
     3061 tttctgagca agaagcatgc ctattccgag tgctgagtac gagtggtcag tggtcggtgg
     3121 tcagtggtca gtgttgagtg tggcgaagga ctcgcgggtg atggcgtagc gggctacgtc
     3181 gaggccgtag tggtgggcgg gaccctggta ttccatgccc agcttttcca tgacgcgggt
     3241 ggaggcgcgg ttggcgggga cggccaccgc gacgatgcgc ggcaggtgca ggtgctcgaa
     3301 gccgaagcgc aggcaggcgc gcgccgcctc ggtcgccaat ccctggcccc agtacggctc
     3361 gtcgatcagg taggccactt cgacctcgtc cgtgtcttcc agcgggaaca gcccggcctg
     3421 cccgatcagc gcgccggtgg ccttgtcttc cagggcgaac aggccgaagc cgcgcatctc
     3481 ccagtcctgg atgcgccgct cgatacccgc gggaatccgt tcgcgcggga tcggctgccc
     3541 gccgggcatg aagcgcatca ccgcgggctt gctgaagatc gcggccagcg cgtccagatc
     3601 gtcgcgccgg aacagccgca gccgcaagcg cggcgtctca aactcaggca tgtgcaggtc
     3661 tcccatgtca gccgtcagcc gtcagccgcc agccgtcagc cgtcagccgt cagctatgtg
     3721 ctacgcaccg cttcgcgatc aggcattgtc gatctcagcc gccttatgcg tagccgcgcg
     3781 atttatcgcg ccgtactcgt tggtcgtcag cgtgctgctg acccggcagg tgtaggcgtg
     3841 ggccgccact acagaaaagg ccgcttactc gtctcctgac ggctgatggc tgacggctaa
     3901 ccgctgcccg ctcgcccgcc accaggcgtg gaactccggt aacgaggcga cgaccagatc
     3961 ggcggcgggc gcgggtgcgc cgggcggcag cggcgccgcc tggagcgcga cggcgaacat
     4021 ccccgcggcg cgggcggcct ccaggccacg cgccgagtcc tcgatcacca ggcactgggc
     4081 cggatcgagg cccagcaggc tcgccgcgcg caggaagatg tcgggcgcgg gtttgccgcg
     4141 agtcacctgc tcgccgctca ccaccacggc gaaatagtcg cgcacgccga tgcagcccag
     4201 cgtgacctgg acccagcgcg ccgccgaact ggacgccacg gccagcggca cccccgccgc
     4261 ccgcagggcg gccagcaacg gcagcaggcc cggcatgggc acgccctgct cagcgaccac
     4321 cgcgcacacc gcctcgtcat agcgggtcat gtagctggcc ggcgggtcgg gcaacccgcg
     4381 cgcggccaca atccgctccc aggcgtaggc cgaactggcg ccgatgtaag tgcgatactc
     4441 cgggtcgtcc acgcgctgct gcagtggggc gagcacggcg ttcatggccg cgtgatgcag
     4501 cggctcgctg tcgatcaaca cgccgtccat atcgaagata atcgcctgga tgttcatcgc
     4561 ggcctcgcgg gcaccggcgc catgaccagc aggtcggcct gccgttccac ccgcagcacg
     4621 aagtcgaagc ggtccagcgc cgtgcagcag cacaggtcgg ccacgaactc gacgcgctcg
     4681 gcctcggtga agtcgtggcc aaagcccgca tcgctcatgc gcatcagccc ggcgtcgtgc
     4741 gtgcgcgcca gcagcgcggc caccggcggg cgctcgccgc gcaggagcgc ggccaggtac
     4801 tcggcgcacg cctggtcctc gtcctcctgc cccgtcgtga ccaacgtcac ctcgccggca
     4861 ccctgcgccc ggatatggcg caccgtggcg gcggcgcaga cgaaattgcc gcccagcagc
     4921 agtgacgccc gcgcgctacg caccagcccc tgcgtgcccg cgctggtgcg ctggatcagg
     4981 cgcgtgccgt gcaggtcgcg cccgataagc gcggcgggcg agttgccgaa atcgaagccg
     5041 ggcacgggca ggccgcccac ctcacccatc accagcgcgc cgggaaagcg cgcccgcagc
     5101 gccagcgcct cctcgaccgt gcccacgggc acgatgtcgc gcacgcccgc cgcgaaggca
     5161 taggccgccg tggtcatggc gcggatcacg tcgatgacga caacggtgcc ggcggcctcg
     5221 gcgcaggtat cgagcgtcgc aaagtggatc tgcatcaggg cctcaccgcg tggcgtgagc
     5281 gcgacgcacg gcggtcgcgt gcgcggcctg ttccacggtc tcgtccaggg gggccaggtc
     5341 cacgtcgccg tggcggcggt gcaaggcctg gcgcagcagg tagtcggtga aacgcgggtt
     5401 cttaggcagc agcgagccgt ggatccagca gccgtaggcc gtcttgtaga tcgcgccctc
     5461 ctgcccgtcg cggccgttgt tgccgtagcc gacgatgttg cggcccagcg ggcggcaacc
     5521 ggggttgagg aaggtcagcc cgctgtggtt ctcgaagccg accagttcgc ccacgaagtc
     5581 ggtctcgacc acgacgttgc cgatgaagcg cgtatccccc gccaccgaat aggcgtcgaa
     5641 caggccggtg cccggcaact cctgcccgtc cgccgggcgg tagtagtgct gcaacagctc
     5701 gtagccgccg cagacgctga gcagcgccgc gccgttctcg acggcttcgc gcaactgggc
     5761 gcccttggtg cccgctaagt cctgggccac gctgatctgc tggcgatcct ggccgccgcc
     5821 gaagaagtag aaatcgtcgc gcgttatgtc aagcgattcg ccgatggtcg tcgtgtggac
     5881 atggacggtg ataccccgcc actcggcccg gcgctgcatg gcgataatat tgccccggtc
     5941 gccgtagatg ttcatcaggt cggggtagag gaagttgatg tgcagatcca taagcgtaga
     6001 tacctcggcg gcgcgccgct actcctgacc cggcgggcgc tcggtcacaa tgtgcacgcc
     6061 ctggcgggtg atgtggaacg ggatggtgct gaagccggcc tcgtgcagcg ccgcggcgac
     6121 cgcgtcgcgg gtgccgggcg cgcaggccag cagcacgcag ccgcccccgc ccgcgccggt
     6181 cgccttgccg ccggtagccc cgtgggcggc ggccacggcc agcacctgct cgatgcgcgg
     6241 cgtggacacc gatgggtgca gcgcgcgaaa ggcgtgccac gcggcggtca tgtgcggccc
     6301 caacccgcgg atgtcgcccg cctccagcat cgcctcgatc tcggcggcga tctcgcgcag
     6361 gcggcgcaga tgcccggtga cggcggcatc gcccgcgaca tagcggcgca tcacgccgtc
     6421 gagcaccgcc ccggagatgc gcgaggtgcc gctgtagcag agcagcagcg actcgttcag
     6481 cgcctggacc gtctccggcg ccacggccag gtagcggcgg cccacctcgc ctgcggcgaa
     6541 gcggtagctc tgcaccgccc cgaaggtggc ggcgtactgg tcctgcccgc cgcccacgtt
     6601 gtgcagcttg cctgtctcgt ggcggatggc cgcctgggcc aggtcggcgc ccgccggcac
     6661 gccctcgccg ctgatctcgt gcagggcgta gagcaaggcc acgctcaccg ccgccgacgc
     6721 ccccaggccc gacccggcgg gcacgtcggt ctgcacctgc acctccacgc cctggcggat
     6781 gccggttagc tcaattgccg tggtcagcag gcgcaggggc gtgtcgtggc gcagcgcggc
     6841 aacggagggg taggtctcct gtatgcccag gtcggcggag gcgagcacca cctggccgtc
     6901 agtgcgccgg gccacgcgca ccacggcata cttgtcgatg gcggcgttga ccacgtagcc
     6961 ccattcctcg gtgcagaagg gcgccacatc ggacggccct ccggcgaaat cgacccgcac
     7021 cggcgccctg acgacgatct ggtccttcac aatgcgctcc ccgccctatt gctcccagaa
     7081 ctggctgacc caacctcgcc gctgcaagag cgcgcggaag tcgagcatcg ccgtataggt
     7141 cggcagcaca tagagcgttt cgcccgcggg caacgcggcc agcgtcgcct ccagcgcggc
     7201 gtccaactcc tcgacgaccc gaatgcgtgc cgggtcgagc ccggcgtact tgagccggtt
     7261 cgccatgtcg gtggcgcgga tcccggcgca ggtggccgag gctacccggc ccggttcgcc
     7321 cagcacctcg aagtccacgt cccacagcca cgacacatcg cgcccgtcgg cgatcaggtc
     7381 gttgatgatg atcagcaggt ggcggggcgg ggcctcgtgc tgcggcggga agagcatccg
     7441 cagcacttcg ttgaagccga ccgggttctt gaccagcgcc agcagcaggt gcttgccgct
     7501 ggtccccgcc gccacgcgct cgatgcgccc gaaagcggcg cggaagccgc ccaggccccg
     7561 ccggattata tcggcgctca ggcccagcgt gagcgcggcg gcgcaggcgc ccagcccgtt
     7621 gtaggcgtta tagaagccgg gcacggccag ttccaccggg aaggtctgct cccccgcgcg
     7681 caccgtgagt cgggtcgcct ccgtgccctg caacgcaacc gactcggcga caatatcggg
     7741 cgcgggccgg acgaagccgc aggccgggca gcggtagtgc cccaggtgcg agagcaagat
     7801 cgcgctgtag tcgagccggt gcccgcagac cgggcaatcg atggcgtcgg cggcatgggg
     7861 cagcgcggcc agggcctggc cggtatcggc gatgccgtaa tagcacaccc gcgcggccag
     7921 gctgcgcccc agcacggcca ccgccggatc gtcggcgttc aggacgaccg tgctgcgcgc
     7981 gtcgagcgtc gccagggtct cgcgccagcg gcggtggatg tggtcgatct cgccgtagcg
     8041 gtccaactgg tcgcggaaca ggttgttgat cagcaggagc cgggggcgca cctgggccac
     8101 cacggcgggc accgtggcct cgtccacctc gaagaggccg ctgtcggcgt ggaggttgcc
     8161 gccgaggttg ctttcctgca ccagcgtggc gatcagcccg cgcaccaggt tggagccgga
     8221 gcggttgtgg atcgcgtgcc agcccgccgc gtcgagaatc gcgcccagca tgcggctggt
     8281 ggtcgtcttg ccgttggtgc cggtgacgat gatcaccccg cgccgcagtt ggcgggtcag
     8341 gcggcgcagg atgcgcggcg acacccgttc ggcgaccagg ccgggcagcg ccgtgccgcc
     8401 cccgcgccgc agcaggcgcg ccgcctggcc cgcgccctgc gcggcggcga tggcggccat
     8461 ctcgcgcggg ccgatgcctt ttaccatcgc accaggtctc cgcgcgcgcc gggatgcagc
     8521 acccgcgcgc acgacgggca atagacgaac tccagcccct gcttgaccgt gcgcagcgtc
     8581 ccggcgggca cggtgacgcg gcaggcctgg cacacgtcgt gctccagccc ggcgatggcg
     8641 gtgccgtgct tggccttgcg cagcttctcg tagagttgca ggtcggccca ggcgatcccg
     8701 gccaccatct tctcgcgcct ggtgcgtagc acgcgcaact ggccgtcgat ggcgtccttt
     8761 tcttcgagca ggccctcctg gttgcgctgc cactcggtct cggccacctc caggtcctgg
     8821 gccgcggcgt ccaccgccgc ctgcgcctgg tccacagcgt ccatcgcctc cagcatcaca
     8881 tcttcgcggg ccgagcgcac ctctttcgtg gcggtgatgt cgtgctgaat cgccaccagt
     8941 tccttggggt tcttgaccga gccgtcgtag aggcgtttgc tttgcagggt caggtgcgcg
     9001 gtcatctcgt ccacctcgtg gctcagcacg cgcaggcgtt cctgggccac ggccagcgcc
     9061 gtcgccgcct ccgcatggcg ctcgcgggcg agcttgaggc tgtcggtctc gtcgagggca
     9121 tgtttaatcg ccagtgcccg gcgcagcagt tcgtcggact ggctatcgac ctgctgtaac
     9181 tcgaatagct gggtggtccg cgccatggca ctccctcttc cttctcgctg taacccgttc
     9241 acgctcattc gcagtcgtaa gataccagat ccaccccgca atggcaagac cggcgcgagt
     9301 gctgagtgct gagtgctgag tg
//
LOCUS       DASUYC010001140         3267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_661449,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001140 DASUYC010000000
VERSION     DASUYC010001140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661449"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1202
                     /locus_tag="VF276_18625"
     CDS             <1..1202
                     /locus_tag="VF276_18625"
                     /inference="COORDINATES: protein motif:HMM:NF024298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX6608922.1"
                     /translation="QKDYWRVLTGKETAMNTVAFAPDGQRLATNMGEQVLLFRWRDST
                     PLARFQGHQGQVTTLAFAPDGQTLASGATDATVRLWAIPPLPTPATPAPPTATPDPAL
                     LHTWLGHGAKVSSVAFAPDGQEVASAGDDRRVHLWSLVPGVHPLALDPQRYRVTSVAW
                     SPDGQILAAGGEDSTIHLWRQSDHNLLRTLTADAGTIYSLVFTANGETLISGSATRLI
                     HIWRVSDGTAIRVLEAQPKPVLSLALAPGSQVLATASSEATVRLWRISDGAPLDPITI
                     PGRRAFAVAFTPDEQVLAVGLDNGTIQLWRVADRTLLRTLHGHKDVILSVAFDPTGQV
                     LASGSYDHTIKLWRVGDGTLIQTLMGHTEGVYSVAFAPDGRTLASGSADTTVRLWQVA
                     PPGATSP"
     gene            complement(1268..1663)
                     /locus_tag="VF276_18630"
     CDS             complement(1268..1663)
                     /locus_tag="VF276_18630"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX6608923.1"
                     /translation="MSVESTRAVMTAYLDHDDLSRVAENAVYRVMGTGYEAMGREGVR
                     QLLRHFYQEAFDARAQTRNLIYAEDHAVLEADFIGKHIGNFAGIEPTGQVISVPLCVV
                     YDLAGDQITQARLYFETDALFRQLRGAFI"
     gene            complement(1716..2774)
                     /locus_tag="VF276_18635"
     CDS             complement(1716..2774)
                     /locus_tag="VF276_18635"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEX6608924.1"
                     /translation="MQLRIPGPTPLPETVRAAGARQMINHRGQAFHELLRDVTERAKG
                     FYQTQNDLLFFPAAGTGGLEASIVNCFSPGDTVLSLSSGHFADRYADIAAAYGLEVRR
                     VAFEWGQAVDPDAARRALDAHPEVRGVLLTHNETSTGVQNPVEAIGALAREYDKLLLV
                     DSISALGATDLRTDAWGLDVVVTASQKAWMTPPGLAMLSVSPRAWDANRRARLPRYYF
                     DFAAARKYAERWETPYTPAVSLLFQLQAALQLMDEEGRDAVFARHEALRDYVRGRLRA
                     LGLPLLAADAVASRTVTAFAVPDARPILAALRRQGIELAGGQGSLEGRLIRIGHVGWA
                     TEADMREVLDRLAEVLAA"
     gene            complement(2775..>3267)
                     /locus_tag="VF276_18640"
     CDS             complement(2775..>3267)
                     /locus_tag="VF276_18640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608925.1"
                     /translation="QALEPLADTLGLCRCKVRANELHFDENGGYAGFDRTNPLWQAGG
                     KAMVVRELAPTRRGVLLAGDGATDAEARHAAELFVGLGGVANHETVRRTADVYIKCKS
                     LAPLLVLAAGREGCARLLQHPDYRDLVIKGLGLLVRGGCVEWKDEYAAFGLKVRRYAL
                     EGI"
BASE COUNT          546 a         1130 c         1082 g          509 t
ORIGIN      
        1 tgcaaaagga ctattggcga gtattgaccg gaaaagagac cgcgatgaac acggtggcgt
       61 tcgctcccga tggacaacgc ctggcaacga acatgggaga acaggttttg ttattccgct
      121 ggcgcgatag caccccgcta gcccgcttcc agggacacca agggcaggtg accactctgg
      181 cgttcgcccc agatggacaa acgctggctt ctggggccac ggacgcgaca gtgcgcttgt
      241 gggcgattcc gcccttgccg accccggcca cgccggcgcc gccgacggcg acccccgatc
      301 cggcgctgct acacacctgg ctggggcacg gcgccaaagt ctccagtgtg gccttcgccc
      361 ccgacgggca ggaggtggcg tcggcgggag acgaccggcg cgtccatctg tggagtttgg
      421 tgccgggggt ccacccgctg gccctcgacc cgcaacggta tcgggtgacg agtgtggcct
      481 ggtcgccgga tgggcagatc ctcgcggccg gcggggagga cagcacgatc cacctgtggc
      541 gccagagcga tcacaacctg ctccgcaccc taacagccga tgcaggaacc atctacagtt
      601 tggttttcac cgcgaatggc gaaacactca tttccggctc ggcaacgcgc ttgatccaca
      661 tctggcgggt gagcgatgga accgcgattc gggttctgga agcacaaccg aagccggtgt
      721 tgagtcttgc cttggctcct ggtagccagg tgctggccac cgccagcagc gaggccactg
      781 tgcggctctg gcggatcagc gatggcgccc cgctggaccc gatcacgata ccagggcggc
      841 gggcgttcgc cgtcgccttc actcccgatg aacaagtcct cgcggttggt ttggacaatg
      901 gcaccatcca gttatggcgg gtggccgatc ggacgttgct ccgcacgttg cacggccata
      961 aggacgtgat actcagtgtg gcgttcgatc ccacgggcca ggtacttgcc tccggctctt
     1021 acgaccacac catcaagctg tggcgggtcg gcgatggaac gctgatccaa accctgatgg
     1081 gacacacgga aggggtctac agtgtggcct tcgcccccga tgggcggact ctcgcgtcgg
     1141 gatcggcgga cacgacagtg cgcctgtggc aggtggcacc tcccggcgcc acctccccat
     1201 aaaacccgag caagttttgt ggtgacagct aaaggggcgc acggccacgc gagagggggc
     1261 gacgcggtta gatgaaggcg ccgcgcaact ggcggaacag ggcatcggtc tcgaaataga
     1321 ggcgcgcctg ggtgatctgg tcgccggcca ggtcgtagac cacgcagagc ggcacggaga
     1381 tcacctggcc ggtcggctcg atgccggcaa agttgccgat atgcttgccg atgaaatcag
     1441 cctccagcac ggcatgatcc tcggcgtaga tcaggttgcg ggtctgcgcc cgcgcgtcga
     1501 aagcttcctg gtagaagtgg cgcagcagtt ggcgcacacc ctcgcggccc atcgcctcgt
     1561 agccggtgcc catgacccgg tagacggcat tctcggccac ccggctcagg tcgtcgtggt
     1621 ccaggtaggc ggtcatgacc gcccgcgtac tctccaccga catggcagac tcccttccgc
     1681 gattcgctgg tgcggcaggc gcccgcaacc ctgcgctacg ccgccagtac ctcggccagc
     1741 cggtcgagca cctcgcgcat atcggcctcg gtggcccagc ccacgtggcc gatgcggatc
     1801 agccggcctt cgagcgaccc ctggcccccg gccagttcga tgccctggcg gcgcaacgca
     1861 gccaggatcg ggcgcgcatc gggcacggcg aacgccgtca cggtgcgcga ggccacggcg
     1921 tcggcggcca gcaatggcag gcccagcgcg cgcagccgtc cgcgcacata gtcgcgcagc
     1981 gcctcgtgac gcgcgaacac ggcgtcccgc ccctcctcgt ccatcagttg cagcgcggcc
     2041 tgtagttgga acagcagcga gacggcgggc gtgtacggcg tctcccagcg ctcggcatac
     2101 ttgcgcgccg cggcgaaatc gaaataatag cgcggcaacc gcgcccgccg gttcgcgtcc
     2161 caggcgcgcg ggctgaccga cagcatcgcc aggcccggcg gcgtcatcca cgccttctgc
     2221 gaggcagtga cgaccacgtc aaggccccag gcatccgtcc gcaggtcggt cgcgcccagc
     2281 gccgagatgc tgtccaccag cagcagctta tcgtactccc gcgccagtgc gccgatggcc
     2341 tccaccgggt tctgcacgcc ggtcgaggtc tcgttgtggg tcagcagcac gccgcgtacc
     2401 tccggatgcg cgtccagggc ccggcgcgcg gcgtcggggt ccacggcctg gccccactcg
     2461 aacgccacgc ggcgcacctc cagcccgtag gccgcagcga tatcggcgta gcggtcggcg
     2521 aagtggccgc tgctgagcga gagaacggtg tcgcccggcg agaagcagtt gacgatcgag
     2581 gcttccagcc cgccggtccc cgccgccggg aaaaagagca gatcgttttg cgtctgataa
     2641 aagcctttgg cccgctcggt cacgtcgcgc agcaactcgt ggaacgcctg gccccggtgg
     2701 ttgatcatct ggcgcgcgcc ggcggcacgc accgtttcag gcagcggcgt cggtcccggt
     2761 atccgcaatt gcatctaaat gccctccagc gcatagcggc ggaccttcag cccaaacgcc
     2821 gcgtactcat ctttccattc gacacagccg ccgcgcacca gcaggcccag gcccttgatc
     2881 accaggtcac ggtagtcggg gtgctgcaac agccgcgcgc agccctcgcg cccggcggcc
     2941 agcaccagca gcggcgccag gctcttacat ttaatataca cgtcggcggt gcggcgcacg
     3001 gtctcatggt tggcgacccc gcccaggccg acgaacagtt cggcggcgtg gcgggcctcg
     3061 gcgtcggtcg cgccgtcgcc ggccaggagc acaccccggc gggtgggcgc cagttcgcgc
     3121 accaccatgg ctttgccgcc cgcctgccag agcgggttgg tgcggtcgaa cccggcatag
     3181 ccgccgttct cgtcgaagtg caactcattc gcacggacct tgcagcggca gaggcccagc
     3241 gtgtcggcca gtggctccag cgcctgc
//
LOCUS       DASUYC010001141         4775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_663307,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001141 DASUYC010000000
VERSION     DASUYC010001141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663307"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1058
                     /locus_tag="VF276_18645"
     CDS             <1..1058
                     /locus_tag="VF276_18645"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6608926.1"
                     /translation="PRDTTTMVKQLLELAPSTQVLLLAEDTDAPDMRRAVLAGARGIL
                     RKPLNAEELLGTILEVFESEVARRERIAELARQRKAKATRGRVIVIFSPKGGVGCTVL
                     ACNLAIALRRLTNKRVALIDYSLQFGTIGTQLNIQSAHSVAELAPHEEDLDNTILDDV
                     MVQHTSGVKVLLPPISPDQVEMITTESLTGILEGLRSHFDYIVVDTWHSVEDATLAIM
                     ESADNLLLVTTPEVPALSTARRFLDMLKRYPQLLYKPQLIVNRHPSKGAVALTEIEKS
                     IGLQALATIPSDGSVITLSINEGIPLFEKNVSSVAVRNLSKLAEALALPADIQSPIAA
                     GNSNWLGTLRRRSFPQS"
     gene            1099..2625
                     /locus_tag="VF276_18650"
     CDS             1099..2625
                     /locus_tag="VF276_18650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEX6608927.1"
                     /translation="MPIIRRKSGAPPPLAREMPPGGPPSGGLIHNGIAPRSLPGDAST
                     GGPTFRNSFGVTGGTSGGLATRASGSRGSNGIALPRSSRVDLEQRLHHKIIAELRDSV
                     DLNDPMAVRAQIERLFNHYMAEEDVVLNRIERSKVFDQVCAEILGYGPIQQLINSDMV
                     SEIMVNGAQQVWVERQGKLTLTDITFKDDEHVMRIISRIVAPLGRRIDESSPMVDARL
                     PDGSRVNAIIPPLSLIGPVLTIRKFSRVPLGVDDLLSLGTLTDPAVIFLESAVRAGLN
                     VVVAGGTSSGKTTLLNVLSSFIPPDERIITIEDAAELQLQQVHVIPLESRPASIEGQH
                     EISIRDLVVNCLRMRPDRIVVGECRDKEALDMLQAMNTGHDGCMTTVHSNSPRDALSR
                     VETMALMASGDLPLRAVREQIAAAFDIIVYMERMEDGSRKVTYITEIQGFEQDTILLQ
                     NVFQLDWVERGGQRMRELVPTGIRPQIMRKIERSRIFLAPDFFQPPAELVTTPHRLGR
                     "
     gene            2661..3635
                     /locus_tag="VF276_18655"
     CDS             2661..3635
                     /locus_tag="VF276_18655"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX6608928.1"
                     /translation="MLIILAGLLVGLAVFGILAGIVITLTSPQGRVNARVRQYVAGDG
                     PATVTQSPVTRQARRHNDLIAQLDARWEQRRTGQALVTELERGGLNLTVSEFLLLRIS
                     AAVGVALFLALLAGPIWWLVLLPGALLGWRLPRTYLRWSARRRLNKLDSQLPDILNIL
                     AGSIRTGSSLLQALERIAREAEEPSRSEYLRVVRAISLGAPLETALLGLSERMPTEDV
                     DMLVTAIGIQQQTGGNLGQILDLIATTVRERHRILREIEVLSATQRASALLLALLPVG
                     IIGILFLISPSYIARLFQPGLILILPITGVVLLAIGFFIMNRIASIDV"
     gene            3652..>4775
                     /locus_tag="VF276_18660"
     CDS             3652..>4775
                     /locus_tag="VF276_18660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX6608929.1"
                     /translation="MPMLKRVLPAILLALLLLPTTALAQTPTRSMKLNQIRGSGWPAV
                     AVNFNLRSLNNTPLGDIKPDQFVIEENGVAQQVTSVALAHDIGIPLSVVLVLDTSGSM
                     QGPKLAAAQAAAAGFMDGLGAGDEVALLPFNSRVLPAIPFTSDKQKIADGLNSQVATG
                     NTLLYDALYAAAQLVNTSKPDNRRAIVLLTDGQDTGSIHQLGFAVAAARTAGATVYTI
                     GVGGDTSDRVMAAMSDPTGGRYAKAAGPDELKGIYEELARELAGQFLLTYKSTTHIAK
                     AYETILVRMRYTTPAGEVLVQETRYRPPLAAVLPAEAIPTPTAVPIAQVPLPPGLPAS
                     AGGVTGPGTGNGPGAIDLSSRSTAGLIAAALAGMAVLLVFG"
BASE COUNT          814 a         1687 c         1545 g          729 t
ORIGIN      
        1 atccgcgcga cacgaccacc atggtcaagc aactgctgga gctggcgccc agcacgcagg
       61 tgctgctgct ggccgaggat accgatgcgc ccgacatgcg ccgcgccgtg ctggcggggg
      121 cgcgcggcat tctgcgcaag ccgctcaacg ccgaggagtt gctgggcacc atcctcgaag
      181 tcttcgagag cgaggtggcc cggcgcgagc gcatcgccga actggcccgc cagcgcaagg
      241 ccaaggccac ccgcgggcgc gtgatcgtga tcttcagccc caagggcggc gtcggctgca
      301 cggtgctggc ctgcaacctg gcgatcgccc tgcgccgcct caccaacaag cgcgtggccc
      361 tgatcgacta tagcctgcaa ttcggcacca tcggcacgca actcaacatc cagtcggcgc
      421 atagcgtcgc cgaactcgcg ccccatgagg aggatctcga caacacgatc ctcgacgacg
      481 tcatggtgca gcatacgtcg ggagtcaagg tgctgctgcc gcccatctcg cccgaccagg
      541 tggagatgat caccaccgag agcctcaccg gcatcctgga gggcctgcgc agccacttcg
      601 actatatcgt ggtcgatacc tggcactcgg tggaggacgc cacgctggcg attatggagt
      661 ccgccgataa cctgctgctg gtgacgacgc ccgaagtccc ggcgctgagc acggcgcggc
      721 gcttcctgga catgttgaag cgctacccac aactgctgta caagccgcaa ctgattgtca
      781 accgccatcc cagcaagggc gcggtggcgc tgaccgagat cgaaaagagc attggcctgc
      841 aggcgctcgc cacgattccc agcgacggct cggtaatcac cctgtccatc aacgagggca
      901 tcccgctgtt cgagaaaaac gtgagcagcg tggcggtgcg caacctgagc aaactggccg
      961 aggcgctggc gctgcccgcc gacatccaat cgcccatcgc ggcgggcaac tcgaactggc
     1021 tgggcaccct gcgccgccgc agctttccgc agtcataact cgtaacgaat cgcactatcg
     1081 tctaggggag atgtcgttat gcccatcatc cgtcgtaaat ccggggcccc gcccccgctg
     1141 gcgcgggaga tgccgcccgg cgggccgcca tcgggcgggc tgatccacaa cgggatcgcg
     1201 ccgcgctcgc tgccgggcga tgcgtccacc ggcggcccca ccttccgcaa cagcttcggc
     1261 gtcaccggcg gcaccagcgg gggcctcgcc acgcgcgcca gcggcagccg cggcagcaac
     1321 ggcatcgccc tgccccgctc gtcgcgggtc gatctggaac agcgcctgca ccacaagatc
     1381 atcgccgaac tgcgcgactc ggtcgatttg aacgatccca tggccgtgcg cgcccagatc
     1441 gagcggctgt tcaatcacta catggccgaa gaagatgtgg tcctgaaccg catcgagcgg
     1501 tcgaaagtat tcgaccaggt ctgcgccgag atcctgggct acgggccgat ccagcagttg
     1561 atcaacagcg acatggtcag cgagatcatg gtcaacggcg cgcagcaggt ctgggtcgag
     1621 cggcagggca aactgaccct gaccgacatc accttcaagg acgacgagca tgtgatgcgc
     1681 atcatcagcc gcatcgtggc cccgctgggc cggcgcatcg acgagtcctc gccgatggtc
     1741 gacgcccgcc tgcccgacgg atcgcgcgtc aacgccatca tcccgcccct ctcgctcatc
     1801 ggcccggtgc tgaccatccg caagttcagc cgggtgccgc tgggcgtcga cgacctgctg
     1861 agcctgggca cgctgaccga ccccgccgtg atcttcctgg agtcagccgt gcgcgccggg
     1921 ctcaatgtcg tcgtcgccgg cggcaccagc agcggcaaga ccaccctgct caatgtgctc
     1981 tcgtcgttca tcccgccgga tgagcggatc atcaccatcg aggacgcggc ggaactgcaa
     2041 ctccagcagg tccatgtcat cccgctggag tcgcgcccgg ccagcatcga gggccagcac
     2101 gaaatcagca tccgcgacct ggtggtcaac tgcctgcgca tgcgccccga ccgcatcgtg
     2161 gtcggcgagt gccgcgacaa agaggcgctg gacatgctcc aggccatgaa caccggccat
     2221 gacggctgca tgaccaccgt gcactccaac agcccgcgcg atgcgctgtc gcgcgtcgag
     2281 accatggcgc tgatggcgag cggcgacctg ccgctgcggg cggtccgcga acagattgcc
     2341 gcggcgttcg acatcatcgt ctacatggag cgcatggaag acgggtcgcg caaggtaacg
     2401 tatatcaccg agatccaggg cttcgagcaa gacacgatct tgctgcaaaa cgtgttccag
     2461 ctcgactggg tggagcgggg cggccagcgc atgcgcgaac tggtgccgac cggcatccgg
     2521 ccccagatca tgcgcaagat cgagcggtcg cgcatcttcc tggcgcccga tttcttccag
     2581 ccgccggccg aattggtcac cacgccgcac cggctagggc gctagccggg tgccgcggcc
     2641 acgctagaag ggacgctgcc atgctgatca tcctcgccgg gctgctggtg ggtctggcgg
     2701 tcttcggcat cctggccggg atcgtgatca ccctgaccag cccgcagggg cgggtcaacg
     2761 cccgtgtgcg gcagtatgtg gccggcgacg ggccggcgac ggtcacgcaa agcccggtga
     2821 cccggcaggc gcggcgccac aacgacctga tcgcccaact cgacgcgcgc tgggagcagc
     2881 ggcgcacggg ccaggcgctc gtcaccgagt tggagcgcgg cggcctcaac ctcaccgtga
     2941 gcgagttcct gctgctgcgc atcagcgcgg cggtgggcgt ggcgctgttt ctggcgctgc
     3001 tggccgggcc gatctggtgg ctggtcttgt tgccgggcgc cctgctcggc tggcgcttgc
     3061 cgcgcaccta cctgcgctgg agcgcgcgcc gccgcttgaa caagctcgac agccagctcc
     3121 ccgacatcct gaatatcctg gccggatcga tccgcaccgg cagcagcctg ttgcaggccc
     3181 tggagcgcat cgcgcgcgaa gccgaggagc cgagccggtc ggaatatctg cgcgtggtgc
     3241 gggcgatcag cctgggcgcg ccactcgaaa ccgccctgct gggcctgtcc gagcgcatgc
     3301 ccaccgagga cgtcgatatg ctggttacgg ccatcggcat ccagcagcaa acgggcggca
     3361 acctcggcca gatcctcgat ctgatcgcca cgaccgtccg cgagcgccac cgcatcttgc
     3421 gcgagatcga ggtgctgagc gcgacccagc gggcctcggc gctgctgctg gccctgctgc
     3481 ccgtcggcat catcggcatc ctctttttga ttagccccag ctatatcgcc cggctcttcc
     3541 agcccggtct catcctgatc ctgcccatca ccggggtggt gttgctggca atcggctttt
     3601 tcatcatgaa ccggattgcg tccattgacg tctagtttag cgaagcgacg aatgccgatg
     3661 cttaaacgcg tgctgccggc gatcctgctg gccctgctgc tgctacccac aacggccctg
     3721 gcgcagacgc cgacccgcag catgaagctg aaccagatcc gcggctccgg ctggccggca
     3781 gtggcggtca acttcaacct gcgcagcctg aacaatacgc cgctgggcga catcaagccc
     3841 gaccagttcg tcatcgagga gaacggtgtc gcgcagcagg tgacgagtgt tgcgctggcc
     3901 cacgacatcg gcatcccgct gtcggtggtg ctggtgctcg acaccagcgg cagcatgcag
     3961 ggccccaagc tggccgccgc ccaggcggcg gcggcgggct tcatggacgg cctgggcgcg
     4021 ggcgacgagg tggccctgct gcccttcaac agccgcgtcc tcccggccat ccccttcacc
     4081 agcgataagc agaagatcgc cgacggattg aacagccagg tggcgacggg caacacgctg
     4141 ctgtacgatg cgctctacgc ggcggcccaa ctcgtcaaca cctccaagcc ggacaatcgc
     4201 cgggccatcg tgctgctcac cgacggccag gacacgggca gtatccacca gttggggttt
     4261 gcggtggccg ccgcgcgcac cgccggggcc acggtctaca ccatcggcgt gggcggcgac
     4321 accagcgacc gggtcatggc ggcgatgagc gacccgaccg gcggccgcta tgccaaggcc
     4381 gccggccccg acgaactgaa aggcatctac gaggaactgg cccgcgaact ggccgggcaa
     4441 ttcctcctga cctacaagtc gaccacgcac atcgccaagg cgtacgagac gatcctggtg
     4501 cgcatgcgct ataccacgcc cgcgggcgag gtgctggtcc aggaaacgcg ctaccgcccg
     4561 ccgctggccg cggtgctgcc cgctgaggcc attcccaccc cgaccgccgt gcccattgcc
     4621 caggtgcccc tgccgccggg gctgccggcc tccgcgggcg gcgtgaccgg gccggggacc
     4681 ggcaacggcc ccggcgccat cgacctgagt tcgcgcagca ccgccggcct gatcgcggcg
     4741 gcgctggccg gcatggcggt gttgctggtc ttcgg
//
LOCUS       DASUYC010001142         3743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1354833, whole genome shotgun sequence.
ACCESSION   DASUYC010001142 DASUYC010000000
VERSION     DASUYC010001142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354833"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="VF276_18665"
     CDS             complement(<1..963)
                     /locus_tag="VF276_18665"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015142190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX6608930.1"
                     /translation="MKLAERMHRLGTESAFEVAARARALEAEGRPMIHLEIGEPDFDT
                     PAHIRQAACAALEAGYTHYGPSTGIPELREVLAQHIGAKRGITADPANVIVTPGAKPI
                     MFYAILALVEPGDQVIYPNPGFPIYESMINFIGGEAIPLQLSESRGFAFDVDDLAALI
                     SNRTRMIILNSPHNPTGGALPRELLRGIADLAKQHDLVVLADEIYSDLVYEGEHHSIL
                     EFPDMLERTILLDGFSKTYAMTGWRLGYGLFPAPLIPHISRLIINSVSCTSTFSQKAA
                     VAALTGPTDEVDQMVAAFHRRRELVVGGLNQIPGLSCVWPEGAFY"
     gene            complement(1314..2063)
                     /locus_tag="VF276_18670"
     CDS             complement(1314..2063)
                     /locus_tag="VF276_18670"
                     /inference="COORDINATES: protein motif:HMM:NF019242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HEX6608931.1"
                     /translation="MSTRKPAPGGPKPAPGTPRPRHAVPIAQAKAKRKGGRDLFGLYF
                     ILGGAAFIFLFGAAVWGLRSLNSQAATVLPTATVETVAQVPLPQTDETPLPNFTPPPS
                     PTLPAPDALNSGPFVQSTPVTSVVTDATKLPEPMNLPPQIPQPKIDMSIESVDLGAVK
                     LGAVITREVTIANPGTHDLVIRGLASDCACVSATVAAETIPSGSRTRMVMTYDSTLEP
                     NTSGRYDHIVTIISTDGQEPKKEFLVSVALP"
     gene            complement(2172..3620)
                     /locus_tag="VF276_18675"
     CDS             complement(2172..3620)
                     /locus_tag="VF276_18675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6608932.1"
                     /translation="MQVQGGQARVGGYAQAAALDRQPLSAFHWKLLLLAGIGWMFDAM
                     DVGLITFVLPVLGAEWKLAPDLRGLILSAGFLGMFVGAAVAGTLADRFGRTLVFSSTL
                     GIFAVATGLTALVPSPDVPAGVQAVDLAHLGDYGSLMTSVIILLVLRFLVGLGLGGEL
                     PVASTLVGEWAPAEQRGRLMVLLESFWAYGWIIAAAVGLLLIPALPGGWRIAFALGAI
                     PALYTLILRRSIPESPRYLESRGDAAGARTMMQAAGMQAADVAASGRATAAPGAGQAL
                     RRLFQPGLVRRTVMLWILWFGIVFSYYGIFSWLPTLMTQHSSLVRGFQYTFLITLAQV
                     PGYFSAAWLVERWGRKPTLIAYLIGTALGALLYGYALVDPGVPDAWLVFSGAVLSFFN
                     LGAWGVVYTYTPEQYPTAVRGSGAGWAAAVGRLGGILGPYVPGLWLGAGFGKEGLFAV
                     FTVVVLITAVAVGLLGTETRGRRLEEISAEVE"
BASE COUNT          658 a         1275 c         1264 g          546 t
ORIGIN      
        1 atagaacgcg ccctccggcc agacacagga cagcccgggg atctggttca gcccccccac
       61 gaccagttcg cggcggcgat ggaaggcggc gaccatctgg tccacctcgt cggtcgggcc
      121 ggtgagggca gccaccgcgg ccttctggct gaaggtgctg gtgcaggaga cactgttgat
      181 gatcaggcgg ctgatgtggg ggatgagggg ggcggggaag aggccatagc ccagccgcca
      241 gccggtcatg gcgtaggtct tggaaaagcc gtccagcagg atagtccgtt ccagcatatc
      301 ggggaactcc aggatgctgt ggtgctcgcc ttcatagacc aggtcgctgt agatctcatc
      361 ggccagcacg accagatcgt gctgcttcgc caggtcggcg atgccgcgca gcaactcgcg
      421 gggcagggcg ccgccggtgg ggttgtgcgg gctgttgaga atgatcatgc gcgtgcggtt
      481 cgagatcagc gccgccaggt cgtccacgtc gaaggcgaaa ccgcggctct cgctcagttg
      541 cagcgggatc gcctcgccgc cgataaagtt gatcatgctc tcataaatgg ggaagccggg
      601 attgggatag atcacctgat cgcccggttc caccagcgcc agaatggcgt agaacatgat
      661 cggcttggcg cccggcgtga cgatcacgtt ggcggggtcg gcggtgatgc cgcgcttggc
      721 gccgatatgc tgcgccagca cctcgcgcaa ctccgggatc cccgtggacg ggccgtagtg
      781 ggtatacccg gcctccagcg cggcgcaggc cgcctggcgg atatgggccg gcgtgtcgaa
      841 atcgggttcg ccgatctcca ggtggatcat gggccggccc tcggcctcca gggcgcgggc
      901 gcgcgccgcc acctcaaacg cactctcggt gcccaaccgg tgcatccgtt ccgctaattt
      961 catgcttggc gttctccttt gaactaacca ggaccaaaac ccaaaaagac cgcgcgacag
     1021 cgaacggccc ggcgcacgga gtcgccgccc ttcaaccgcc ggtaacggga gtcattatac
     1081 cccttgcgcc atccgtgggc aagacacgat tgctgatacc cttttggcag ccggatgatc
     1141 accgcagaga cgccgagaac acagagattc agcatggagg gctgcgcggg atcgcgtctc
     1201 attctaatga tctctgcgtc ctcggcgtct ctgcggtgag gaggatcgga caaagaacgg
     1261 cggcccgccg gatcgcgggc cgccgtggcg ggatcacaag gaaggccgcg ccgctacggc
     1321 agcgcaacgg agaccaggaa ttccttcttc ggctcctggc cgtcggtcga gataatcgtc
     1381 acgatatgat cgtagcggcc cgaggtattc ggctccagcg tggagtcgta ggtcatgacc
     1441 atgcgggtgc ggctgccgct ggggatcgtc tcggcggcga ccgtggcgct cacacaggcg
     1501 caatcgctgg ccagaccgcg gatcaccagg tcgtgggtgc cggggttggc aatcgtcact
     1561 tcgcgcgtga tcacggcgcc cagcttaacc gcccccaggt ccaccgactc gatggacatg
     1621 tcgattttgg gctgcgggat ctgcggcggc aagttcatcg gctcgggcag cttcgtggcg
     1681 tcggtcacga ccgaggtgac gggcgtcgac tgcacgaacg ggccggaatt gagcgcgtcg
     1741 ggggcgggca gcgtggggct gggcggcggc gtgaagttgg gcagcggcgt ctcgtcggtt
     1801 tgcggcagcg gcacctgggc cacggtctcg acggtggccg tgggcaggac cgtggccgcc
     1861 tggctgttca ggctacggag cccccacacg gcggcgccga acaggaagat aaacgccgcg
     1921 ccgccgagga taaagtagag accgaagagg tcccggcccc ccttgcgctt ggccttggct
     1981 tgcgcgatgg ggacggcgtg ccgggggcgc ggggtgcccg gtgcgggctt cggtccgccc
     2041 ggcgcaggtt tacgtgtgct catactggct caaaactctt tctggcaagg gtattactca
     2101 acgccaagcg agcgccggat caaccggcgc ccgcaggctt acgtttgtcc attatacccg
     2161 acgcagcggg tctattcaac ctcggcggag atctcctcca ggcggcggcc acgggtttcg
     2221 gtacccagca ggcccacggc caccgccgtg atcagcacca ccacggtgaa gacggcgaac
     2281 agcccttcct tgccgaagcc cgcacccagc cagaggccgg gcacatacgg cccgagaatg
     2341 ccgcccaggc ggcccacggc ggcggcccac ccggcgccgc tgccgcgcac ggcggtcgga
     2401 tattgctccg gtgtataagt atataccacg ccccaggcgc cgaggttgaa aaacgacagc
     2461 accgcgcccg aaaagaccag ccacgcatcg ggcacgcccg gatcgaccag cgcatagccg
     2521 tagagcagcg cgccgagggc cgtgccgatc aggtaggcga taagcgtggg cttgcggccc
     2581 cagcgctcga ccagccaggc ggcgctgaaa tagccgggca cctgggccag cgtgatcagg
     2641 aaggtgtact ggaagccgcg caccaggctg ctgtgctggg tcatcagcgt cggcagccag
     2701 ctaaagatgc cgtaatagct gaagacgatg ccgaaccaga ggatccagag catgaccgtg
     2761 cgccgcacca ggcccggctg gaacaggcgg cgcagcgcct gcccggcgcc cggcgcggcg
     2821 gttgcgcgcc cggacgcggc cacgtccgcc gcctgcatgc ctgccgcctg catcatcgtc
     2881 cgcgcgcccg ccgcgtcgcc ccggctctcc aggtagcgcg gcgactcggg gatgctgcgg
     2941 cgcaggatca gcgtgtaaag cgccgggatg gcgcccagcg cgaaggcgat gcgccagccg
     3001 cccggcagcg cggggatcag cagcaggccg accgctgccg cgatgatcca gccgtaggcc
     3061 cagaagctct ccaacagcac catcaggcgc ccgcgctgct cggcgggcgc ccactcgccc
     3121 accagcgtgc tggcgacggg cagttcgccg cccagcccga gccccaccag gaagcgcagc
     3181 accagcagga tgatcacgct agtcatcagc gagccgtagt cgcccagatg cgccaggtcc
     3241 acggcctgca ccccggcggg cacatcgggc gaaggcacca gcgccgtcag cccggtcgcc
     3301 acggcgaaga taccgagcgt gctgctgaag acgagcgtgc gcccgaagcg gtcggccagc
     3361 gtgcccgcca ccgccgcgcc cacaaacatg cccaggaacc cggcgctgag gatcaggccg
     3421 cgcaggtcgg gcgccagctt ccactccgca cccagcaccg gcagcacgaa cgtgatcagg
     3481 ccgacatcca tcgcatcgaa catccagccg atcccggcca gcagcaacag cttccagtga
     3541 aacgccgaca acggctgccg gtcgagcgcc gccgcctgcg cgtagccccc aaccctggcc
     3601 tgccccccct gcacctgcat cggatctcct ccttgtcccg ccgcctcctg gccccacttg
     3661 cccgctcgcc tacctgccgc ccagcctctt catgaagcgt gccaatcctt gcgggcataa
     3721 aaagaccggg gacgggcgcg gcc
//
LOCUS       DASUYC010001143         3700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1818499, whole genome shotgun sequence.
ACCESSION   DASUYC010001143 DASUYC010000000
VERSION     DASUYC010001143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1818499"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2294)
                     /locus_tag="VF276_18680"
     CDS             complement(<1..2294)
                     /locus_tag="VF276_18680"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6608933.1"
                     /translation="MDPTLAAYIPMDRRQALARGTSLPDRTHGAALFADIAGFTPLTE
                     TLMRRLGARRGGEELTHQLNRVYDALVAEVHRYGGSVLGFSGDAITCWFADGAPEAAP
                     PATLRATASALAMQQVMAPFATVSLPGGDTVSLAMHVTVAAGPARRFVVGDPAIQIID
                     VAAGAALDRMALVEPLAGKGEVILDAEAVVQAGDQLIIQGWRISADTGARGAMVAGLR
                     APVPATPWPPLLAAALRAEDLRPWLLPAVYARLQSERIEFLTELRPVVALFLRLEGID
                     YEGDPAAGATLDAYVRWVQGTLARYEGTLLQFTVDDKGSYFYAAFGAPIAHEDDARRA
                     VAAAFELSQPPSLPAPLRPLQIGLSQGTMRTGAYGGTTCRTYGALGSEVNLASRLMQH
                     AAPGQVLASERVHLLTSETFGWDALPPIRVKGRSSPVAVYSLRGPAAQGPAAPPADMV
                     GRAAEQAALGHALHALRRGTGRVIIVEGDPGIGKSRLLAAARRQATALEISAFTGTGD
                     AVEQLTPYHAWGTIFSRVLDLPPGAPGETQRAHLERRLAALPDQARVAPLLGAVPGIA
                     IPDNESTAGMAGAARAEHTINLLVAILQDVAAHTPTVIILDDAQWLDSASWALALAVS
                     RRVQPLLLIVSTRPLPAPPPDEYTQLRATAAQGHLRLGALPPDDVAAVVAQRLGVTRL
                     PAPVTALIRQRAEGHPFFSEELACALRDAGWISVAGGECRVAPGVDLSAVTFPDTLHG
                     VILSRIDRLAPSQQVTLKTASVIGR"
     gene            2730..3032
                     /locus_tag="VF276_18685"
     CDS             2730..3032
                     /locus_tag="VF276_18685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608934.1"
                     /translation="MDDKMSKEKGSHHKDPGPAPQSADLDQRRRAFVSKLVDRIAADP
                     SLRQALVDNNQAALDASGLGDEYEALRAAYDKLPESERGAHHSHGLCTWFLPKTGG"
     gene            3124..>3700
                     /locus_tag="VF276_18690"
     CDS             3124..>3700
                     /locus_tag="VF276_18690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608935.1"
                     /translation="MSDPSPRFPTPDPALPGWPVAAVFAADLAGWAVTAPVPPDIWTH
                     LAPPGAAPPRQGWKLHLTAGICDAGAVLGRTLPVLLAERVAFKLVASPAHLHVLNGAG
                     YGLAQVGKFLTVYPRDDAQAVRLAAALDAATRGLRGPAIRTDRPLAPGSLVAYRYGSH
                     APQFAYTDTGTVVPAIQAPDGTWVPDDRGPRY"
BASE COUNT          500 a         1317 c         1379 g          504 t
ORIGIN      
        1 cgcccgatca cgctggccgt cttcagcgtc acctgctgcg agggcgccag gcggtcgatg
       61 cggctgagga tgacgccgtg cagcgtgtcg gggaaggtga cggcgctcag gtccacgccg
      121 ggggcgaccc ggcactcgcc gccggccacg ctgatccagc ccgcgtcgcg cagcgcgcag
      181 gccagttcct cgctgaagaa ggggtggcct tcggcgcgct ggcggatcag ggccgtgacg
      241 ggggcaggca ggcgggtgac gccgagccgc tgggccacca cggcggccac atcgtcgggc
      301 ggcagcgcgc ctaaccgcag gtgcccctgg gccgccgtcg cgcgcaactg ggtgtattcg
      361 tcgggcggcg gtgccggcag cggccgcgtg ctcacgatga gcagcagcgg ctggacccgc
      421 cggctcaccg cgagcgccag cgcccaggac gcggaatcga gccactgggc gtcgtcgagg
      481 atgatcacgg tcggcgtgtg ggcggcgaca tcctgcaaga tggcaaccag caggttgatc
      541 gtgtgttcgg cgcgcgccgc cccggccatg cccgcggtgc tttcgttgtc cgggatggcg
      601 atgcccggca ccgcacccag cagcggggcc acccgcgcct ggtccggcag cgcagccagg
      661 cggcgctcca ggtgcgcgcg ctgggtttcg cccggcgcac ccggcggcag gtcgaggacg
      721 cggctgaaga tggtgcccca cgcatggtag ggcgtcagtt gctcgaccgc gtcgccggtg
      781 ccggtgaagg cgctgatttc gagcgccgtg gcctggcggc gggccgccgc gagcaggcgc
      841 gacttgccga tgcccggatc gccctcgacg atgatcacgc ggcctgtgcc acgccgcagg
      901 gcgtgcagtg cgtgccccag tgccgcctgt tcggcggcgc ggcccaccat gtccgccggg
      961 ggcgcggcgg ggccctgagc cgccgggccg cgcaggctat agaccgcgac gggcgaacta
     1021 cggcccttga cgcggatggg cggcagggcg tcccagccga aggtttcgct ggtcagcagg
     1081 tggacgcgct cgctcgccag cacctggccg ggcgccgcat gctgcatcag gcgcgaggcc
     1141 aggttcacct cgctgcccag cgcgccgtag gtgcggcagg tggtgccgcc ataggcgccg
     1201 gtgcgcatgg tgccctggct gaggccgatt tgcagcgggc gcagcggggc cgggaggctt
     1261 gggggttggc tcaactcgaa cgccgccgcc acggcccggc gggcgtcgtc ctcatgggcg
     1321 atgggcgcgc cgaaggcggc gtagaaatag ctgcccttgt cgtccacggt gaattgcagc
     1381 agggtgcctt cgtagcgggc cagcgtgccc tgcacccagc gcacgtaggc gtcgagcgtg
     1441 gccccggcgg ccgggtcgcc ttcgtaatcg atgccttcca ggcgcagaaa gagcgccacc
     1501 accgggcgga gttcggtcag aaactcgatg cgctcgcttt gcagacgcgc atagacggcg
     1561 ggcagcagcc agggccgcaa gtcctcggcg cgcagcgccg cggcgagcag cggcggccag
     1621 ggcgtggcgg gcacgggcgc gcgtagcccc gcgaccatgg cgccgcgcgc ccccgtgtcc
     1681 gcgctgatgc gccagccctg gatgatcagt tgatcgcccg cctggaccac ggcctcggca
     1741 tccaggatga cctcgccttt gccggccagc ggctcgacca gcgccatgcg atccagcgcc
     1801 gccccggcgg ctacgtcgat gatctggatg gccgggtcgc cgacgacgaa gcgacgggcc
     1861 ggcccggcgg cgacggtgac atgcatcgcc aggctcacgg tgtcgccgcc cggcagggac
     1921 accgtggcga agggggccat gacctgctgc atcgccaggg ccgaggccgt ggcgcgcagg
     1981 gtggccgggg gcgcggcctc gggcgcgccg tccgcgaacc agcaggtgat ggcgtcgccg
     2041 ctgaagccga gcacgctgcc gccatagcgg tggacctcgg ccaccagcgc atcgtagacg
     2101 cggttcaact ggtgggtcag ttcctcgccc ccgcggcggg cgcccaggcg gcgcatcagc
     2161 gtctcggtga gcggcgtgaa gccggcaatg tcggcgaaca gcgccgcacc gtgggtgcgg
     2221 tcaggcaagc tggtgccgcg ggccagcgcc tggcggcgat ccatgggaat gtaggcagct
     2281 agagtgggat ccatacgcgc cggtgtacct cactacttga cgatggggag tccgtgcggg
     2341 atggcgccac ggcagggcgg cgtggcgtgg gtcacgctcc cggttcgccc tagtatagca
     2401 cacgctaccc ggtcccgcta ccgggcgatc cgcctttcgt gaataccgat gcccacggcg
     2461 caggccacgc ggcgcgggct gcccgctgcc gacgtccctc catgtctgcg tattttgcac
     2521 tgcaacgaca caatggccct gaataatacg accaccgctc attggtttcg cgggcctcgt
     2581 tccttcgcgg tgaacttatg gacggatggc cgcccggcgc tgttgacggg ccgggcgcca
     2641 cctgctatac tccacgccgc atcgggaccc acggctatag ggtagagggg gggcagcggg
     2701 gagcggcccc gcggaaagga tcgccggcca tggacgacaa gatgagcaaa gagaagggat
     2761 cgcaccacaa agatcccgga ccggccccgc agagcgccga tctggatcag cggcggcgtg
     2821 cgtttgtcag caagctcgtg gaccgcatcg cggcggaccc gtcgttgcgc caggcgctgg
     2881 tggacaacaa ccaggcggcg ctcgacgcca gcggcctggg ggacgagtac gaggcgctgc
     2941 gcgccgccta tgacaagctg cccgagtctg aacgcggcgc gcatcactcg cacggcctct
     3001 gcacctggtt cttgcccaag acagggggat gatccacggc gcgacgcgcc ccgccctgtc
     3061 ggggcgccgc cggtcttgcc acccgccgcg catccggccc ggcgggtggt tctgtatttg
     3121 ccgatgagcg acccttcgcc ccgtttcccc acgcccgacc cggcgctgcc gggctggccc
     3181 gtggccgctg tgtttgccgc ggacctggcc ggctgggcgg tgacggcgcc ggtcccgccc
     3241 gacatctgga cgcacctggc cccgccgggg gccgcgccgc cccgccaggg ctggaagctg
     3301 caccttacgg cgggcatctg cgatgccggt gccgtgctgg gccgtaccct gccggtgctg
     3361 ctggccgagc gggtggcctt caagttggtg gcctcgccgg cgcatctgca tgtgttgaac
     3421 ggcgcgggct acggcctcgc ccaggtgggc aagttcctca ccgtctaccc gcgcgacgac
     3481 gcccaggcgg tgcgcctggc cgccgcgctc gacgcggcca cgcgcgggct gcgcggtccc
     3541 gccatccgca ccgatcggcc cctggcgccc ggcagcctgg tggcctaccg ctacggcagc
     3601 cacgccccgc agttcgccta cacggacacg gggaccgtcg tacccgccat ccaggccccg
     3661 gacggcacct gggtgcccga cgaccgcggc ccgcgctacc
//
LOCUS       DASUYC010001144         2787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_968908,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001144 DASUYC010000000
VERSION     DASUYC010001144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968908"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1513
                     /locus_tag="VF276_18695"
     CDS             <1..1513
                     /locus_tag="VF276_18695"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX6608936.1"
                     /translation="GTVRPRPAAAPPAPPRLELLLGTAAPGVPGDRIEVQAVLGPGER
                     THGVLRFDATAAEMRGLVARLHSRQAADPEVARLGSVLFDALFRDTVGAAYREARGQA
                     RAHKLPLRLVLTSASVEMAALPWEFLYDPSLRHFLALTPDVHLSRNLPSLRPLNALPA
                     ALPLRVLLGVAGPGTYQGHRLFGLDVTAERALIDTGLAALVDAGYLEVHSVTLDGPAS
                     LPDAVRRTQPHIFHYIGHSAVLRDTPLLFGGPTGGEAVPLSGAQLAAALSLAPELQLV
                     LLNSCWSGTAGVQRSLFGLAPAIAEAGVPAVIGWQTGISDRSAPWLAGSLYAELARGA
                     AVDEALTIARLALYANPRVERLAWGLAVGYLRGERTQIVAPPEKTWRLLVIDDEPERA
                     DLLQSRLRGRGLDVVWAEGGMAGLAEVHRARPDVIVLDLKMPGMDGFEVLRRLKANPV
                     TADLPVVVLSSLGADYETALSAYIGGAKYVIPYNGRLDQLEQVLRANLDVPLR"
     gene            1545..>2787
                     /locus_tag="VF276_18700"
     CDS             1545..>2787
                     /locus_tag="VF276_18700"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX6608937.1"
                     /translation="MERVIQILLADDRDRSRDLLIMLRRHYAVHTAHDDTRLLQLLDQ
                     ARADARPYDLALIAHEPPLLGNPQTMHKLRVIAPTTLVVPLVPPGAADLGLELAGVQS
                     PHYLVQPVDPEAAMAVIHDLLAERLAGLARTRAEITRPLVALGEALLRQPDLERALRH
                     LYQELDSLISAEYLYVALYDRRRGRLVYLLFYDHGDYVSAYTRPYELGQGFAEWVIAN
                     DAPLVSGNLPVDAARRGWPAAEPESPFVPASVMVMPLRLGGQVIGVLSAQSARPEAFQ
                     EADATLFGFVADLMSGVVANWWTDLQWQRQDDLLAEFDKTLAAGGSRDAILDRTVTAA
                     RQMTGMDAAALVGVDRRGTARDWFTSPPGSYADQFQDALADLTGYLAATGRSGGSVDH
                     ARTGRILASLGERGVGRVVSHA"
BASE COUNT          377 a          966 c         1023 g          421 t
ORIGIN      
        1 cgggaccgta cggccccgcc cggcagcggc cccccccgct ccgccgcgcc tggaactgtt
       61 gctgggcacc gcggcgccgg gcgtgcccgg cgaccggatc gaagtgcagg cggtgctggg
      121 tcccggcgag cggacccacg gcgtgttgcg cttcgacgcc acggcagccg agatgcgcgg
      181 cctggtggcc cggctgcaca gccgccaggc cgccgatccc gaggtggcgc gcctgggcag
      241 cgtgctgttc gacgcgctgt tccgcgatac cgtgggggcg gcgtatcgcg aggcgcgcgg
      301 gcaggcgcgc gctcataagc tgccgttgcg cctggtgctg acgagcgcgt cggtggaaat
      361 ggccgcgctg ccctgggagt ttctctacga ccccagcctg cgccacttcc tcgccctgac
      421 gcccgatgtc cacctgagcc gcaacctgcc ctcactgcgc ccgctcaacg cgctgcccgc
      481 ggccctgccc ttgcgcgtgc tgctgggcgt ggccgggccg ggcacctacc agggccaccg
      541 gctgttcggg ctcgatgtga cggcggagcg ggcgttgatc gacacggggt tggcggcgct
      601 ggtggacgcg ggctacctgg aggtccacag cgtcaccctc gacggcccgg cgagcctgcc
      661 ggacgcggtg cggcgcaccc agccgcatat cttccactac atcggccact cggcggtgct
      721 gcgcgatacg ccgctgctgt tcggcggccc gacgggcggg gaggcggtgc cgctgagcgg
      781 ggcacaactg gccgccgcgc tcagcctcgc cccggagttg caactggtgc tgctcaacag
      841 ttgctggagc ggcaccgccg gggtgcaacg gagcctgttc gggctggccc cggccatcgc
      901 cgaggcaggg gtgcccgcgg tcatcggctg gcagaccggc atcagcgacc gcagcgcgcc
      961 ctggctggcg ggtagcctct acgccgaact ggcccgcggc gcggcagtgg acgaggcgct
     1021 gaccatcgcc cggctggcgc tctatgccaa ccccagggtg gagcggctgg cctggggcct
     1081 ggccgtcggc tacctgcgcg gggagcgcac gcagatcgtc gcgccgcccg agaagacgtg
     1141 gcggctgctg gtgatcgacg atgaacccga gcgggccgac ctgttacagt cgcggctgcg
     1201 cgggcgcggg ctcgacgtgg tgtgggcgga aggcggcatg gccgggctgg cggaagtcca
     1261 ccgcgcccgg cccgatgtca tcgtgctcga cctgaagatg cccggcatgg acggctttga
     1321 ggtgttgcgc cggttgaagg ccaaccccgt cacggcggac ctgccggttg ttgtactgtc
     1381 gtcgctcggc gcggactatg agaccgcgct gagcgcgtat attgggggcg cgaagtacgt
     1441 gataccgtat aatgggcggc tggaccagtt ggagcaagta ctgcgggcca acctagacgt
     1501 accgctacgc tgaccactag atcggcagaa acaggagtgc gagtgtggaa cgcgtgatac
     1561 agatcctgct tgcggatgac cgcgaccgga gccgggacct tctgatcatg ctgcggcggc
     1621 attatgccgt acataccgcg catgacgata cgcgtttgct gcaactgctc gatcaggcgc
     1681 gcgccgacgc acggccctac gatctcgcgc tcatcgccca tgagccgccg ctactgggca
     1741 acccgcagac gatgcataag ctgcgtgtga ttgcaccgac cacgctggtg gtgccgctgg
     1801 tgccgcccgg tgccgccgac ctcgggctgg aactggccgg agtccagtcg ccgcactacc
     1861 tggtgcagcc ggtggacccc gaagcggcca tggccgtcat ccacgatctg ctggccgagc
     1921 ggctggccgg cctggcgcgc acgcgggccg aaatcacccg cccgctggtg gcgctgggcg
     1981 aggccctgtt gcgccagccc gacctggaac gcgccttgcg ccatctctac caggaactcg
     2041 attccctgat cagcgccgag tatctctatg tggcgctcta cgaccggcgg cgcgggcggc
     2101 tggtctacct gctgttctac gatcacggcg attacgtctc cgcctacacg cggccctatg
     2161 agttgggaca gggcttcgcc gagtgggtga tcgccaatga cgcgcccctg gtcagcggca
     2221 atctgccggt ggacgcggcg cggcgcggct ggcccgcggc ggaacccgaa agcccctttg
     2281 tgcccgcgtc ggtcatggtc atgcccctgc ggctgggcgg gcaggtgatc ggcgtgctga
     2341 gcgcgcagag cgcccggccc gaggcgttcc aggaagccga cgccacgctg ttcggcttcg
     2401 tcgccgacct catgtccggc gtggtcgcca actggtggac cgacctgcaa tggcagcgcc
     2461 aggacgatct gctggccgag ttcgacaaga cgctggccgc gggcggttcg cgcgacgcca
     2521 tcctcgaccg caccgtgacc gcggcgcggc agatgaccgg catggacgcg gcggcactgg
     2581 tgggggtgga ccgccgcggc actgcgcgcg actggttcac gtcgccgccg gggtcgtatg
     2641 ccgaccagtt ccaggatgcg ctggccgacc tgaccggcta cctggcggcc acgggccgca
     2701 gcggcggatc ggtggaccac gcgcgcacgg gccgcatcct ggcgagcctg ggcgagcgcg
     2761 gggtgggccg ggtggtgtcc cacgccc
//
LOCUS       DASUYC010001145         2193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_2049684, whole genome shotgun sequence.
ACCESSION   DASUYC010001145 DASUYC010000000
VERSION     DASUYC010001145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2049684"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1359)
                     /locus_tag="VF276_18705"
     CDS             complement(<1..1359)
                     /locus_tag="VF276_18705"
                     /inference="COORDINATES: protein motif:HMM:NF014895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEX6608938.1"
                     /translation="MDAAGGGPVVLRGVAGAPGQALGPVLVYRPATPVAPVTPIDPAQ
                     ASVEQARVAAALAGAQAELERLAEEVAGALGPEEAAIFSAQALMVTDPLLSDRAAELV
                     AAEHYPADAAILAAADEQAALLAAIDDPYLRERAADVRDVGRRAARIARGDPAPADLR
                     RLPGPVVILAEDLSPSETAGLDRDRVLGIGLSGGGPTSHAAVVARALGVPLVCGLGAF
                     VAHEGQVALLDGDGGRLTLDPGPDALAAHEAWRAARGRAAVADAGLRDLPAETRDGRR
                     IRLAANAGSVADAQQAAAAGAEGIGLLRTEFLFLDHRPADAEQEAAYRAIYAALPGRE
                     VVVRTLDLGGDKPPPYLDFGREANPFLGWRGLRVALDEPDLLRTQLRALLRAGAGRAV
                     HVMFPMVTTRDEWRRARALVDEVGADLAAAGVPAPTDLAVGIMVEVPAAALLAPVFAA
                     EAD"
     gene            complement(1368..2006)
                     /gene="dhaL"
                     /locus_tag="VF276_18710"
     CDS             complement(1368..2006)
                     /gene="dhaL"
                     /locus_tag="VF276_18710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HEX6608939.1"
                     /translation="MDLTPDVLTACLRRIATVLREQRQYLTDLDSPIGDADHGINMDR
                     GFTAVAAKLDGAAGQDPAALLKLVAMTLISTVGGASGPLYGTAFLRAATALAGKPVLT
                     LDDCVAGMTAAVQGIRDRGKATTGEKTMLDALVPAQEALSAAATEGADLAAAFARAAD
                     AAEAGAQATIPLLATKGRASYLGERSIGHQDPGATSSALMLRAVADTLAGAE"
BASE COUNT          249 a          904 c          789 g          251 t
ORIGIN      
        1 atcggcctcg gcggcgaaga ccggcgccag caacgccgcc gccggcacct cgaccatgat
       61 gcccacggcc agatccgtcg gggcgggcac gcccgccgcc gccaggtcgg cgcccacctc
      121 gtccaccagg gcgcgggcgc ggcgccactc gtcgcgcgtc gtgaccatcg ggaacatgac
      181 gtggacggcc cgcccggccc cggcccgcaa cagcgcgcgc agttgcgtgc gtagcaggtc
      241 gggctcgtcg agcgccacgc gcagcccccg ccagcccagg aacgggttcg cctcgcgccc
      301 gaagtcgaga tagggcggcg gcttatcgcc gcccaggtcg agcgtgcgca ccacgacttc
      361 gcggccgggc agcgccgcgt aaatcgcgcg ataggccgct tcctgttcgg cgtcggcggg
      421 ccggtggtcg aggaacagga actcggtgcg cagcaggccg atgccctcgg cgccggctgc
      481 cgcggcttgc tgcgcgtcgg ccaccgaccc ggcgttggcc gccagccgga tgcggcgccc
      541 gtcgcgcgtc tcggctggca ggtcgcgcag cccggcgtcc gccacggcgg cccggccccg
      601 cgcggcccgc cacgcctcgt gcgcggccag cgcgtccggc cccggatcga gcgtcagccg
      661 cccgccgtcg ccgtcgagca gcgcgacctg cccctcgtgc gcgacgaagg cgcccagccc
      721 gcagaccagc ggcaccccca gcgctcgcgc caccaccgcc gcgtgcgagg tcggcccccc
      781 gccgctcagc ccgatgccca gcacccgatc ccggtccagc cccgccgtct ccgacggcga
      841 cagatcctcg gccaggatga ccaccgggcc gggcagccgg cgcaggtcgg cgggggccgg
      901 gtcgccccgc gcgatgcgcg ccgcccgccg gcccacgtcg cgcacgtcgg cggcccgctc
      961 gcgcagatac gggtcgtcga tggccgccag cagcgccgcc tgttcgtccg ccgcggccag
     1021 gatcgcggcg tccgccgggt agtgctcggc ggccaccagt tcggcggcgc ggtcgctcag
     1081 cagcgggtcc gtgaccatca gcgcctgggc cgagaagatg gccgcctcct ccggcccgag
     1141 ggcgccggcc acttcctcgg ccagccgctc cagttcggcc tgcgcgccgg ccagcgccgc
     1201 cgccacgcgc gcctgctcga cgctcgcctg ggccgggtcg atgggcgtga ccggcgcgac
     1261 gggcgtggcc gggcgataga ccagcaccgg ccccagcgcc tgccccggcg ccccggccac
     1321 cccgcgcagc accaccggcc cgccgcccgc ggcgtccacc gcccccccta ctccgccccg
     1381 gccagcgtgt cggcgacggc gcgcagcatc agcgccgacg acgtggcgcc ggggtcctgg
     1441 tggccgatgc tgcgctcgcc caggtagctg gcccggccct tggtcgccag cagcgggatg
     1501 gtcgcctgcg ccccggcctc ggcggcgtcc gcggcccgcg cgaaggccgc ggccagatcc
     1561 gcgccctccg tggcggcggc actcagcgcc tcctgcgcgg gcaccagcgc gtccagcatc
     1621 gtcttctcgc cggtggtggc cttgccccgg tcgcggatgc cctgcaccgc ggcggtcatc
     1681 ccggccacgc agtcgtccag cgtcagcacg ggcttgccgg ccagcgcggt cgctgcgcgc
     1741 aagaaggccg tgccgtagag cggccccgac gccccgccca ccgtcgagat cagcgtcatg
     1801 gcgaccagct tgagcagcgc ggcggggtct tgcccggcgg cgccgtcgag cttggcggcc
     1861 acggcggtga agccgcggtc catgttgatg ccgtggtcgg cgtcgccgat gggcgagtcg
     1921 aggtcggtca ggtactggcg ctgctcgcgc agcacagtcg cgatgcgccg caggcaggcg
     1981 gtgagtacgt cgggggtcaa atccatacgg ctgcgatcct cctcatgtgt gctaacgctg
     2041 cccggtgggc ggcgcgagcc ggtttgaaac cggcccctac gccgccaccc ggtcatcccg
     2101 gcctcggcgg caggccggtt gcaaacccgc ccctaccccg ccacccggtc atcccggcct
     2161 cggcggcagg ccggtttgaa accggcccct acg
//
LOCUS       DASUYC010001146         2274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_432955,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001146 DASUYC010000000
VERSION     DASUYC010001146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432955"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="VF276_18715"
     CDS             complement(<1..639)
                     /locus_tag="VF276_18715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004828897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX6608940.1"
                     /translation="MAKTADIAPTAEPAAAVADSAGPAGAPAGDRPAKPARRTQAERS
                     EAMRERLVEATLKCLVSEGYAGTTVSKIVATAQVSRGAPVHHFPHKAALIEAAAEKLV
                     RHFYIELGRIMLSLEDSDDRLHKLITVGWRNLMATPYTTALLEILVASRHEPELAQVM
                     RMLWAACYDTTQTAANHYLEPVRPGEDVSDFMILTQWLLRGMAEDYQLTGQHP"
     gene            886..2061
                     /locus_tag="VF276_18720"
     CDS             886..2061
                     /locus_tag="VF276_18720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004732268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6608941.1"
                     /translation="MSAVHIPPHVAPMIPRTLFNADHEAFRDTARRFFETEIAPFHEK
                     WEEQQHLDRVLWNKAGELGLLCCTMPEEYGGSGVDRLYSMILMEEQARVGDSASGFSL
                     HSDIVANYINNFGNEEQKKTWLPKMASGEAVGAIAMTEPGTGSDLQSIRTTAVADGDD
                     YIVNGSKIFITNGYLCDVAVVAVKTGDINGGAQSVSLLIIEANRPGFSKGKPLKKVGM
                     KGQDTAELFFDNVRVPKSNLLGMEGMGFIMLMKELAWERMMIGIMCAAGAEFALDTTV
                     EYVKQRKVFGKPVNAYQYTRFKLAEMRSEVQIARVFVDRCMEQVLKNELSVEAACAAK
                     YWVSDLLSKVVDECVQLHGGYGFMLEYPIARAYIDTRANRIYGGTNEIMKELISRSI"
BASE COUNT          386 a          744 c          754 g          390 t
ORIGIN      
        1 ggggtgctgt ccggtgagct ggtagtcctc ggccatgccg cgcagcagcc actgcgtcag
       61 gatcatgaag tccgacacgt cctcgcccgg gcgcaccggt tccaggtagt ggttggcggc
      121 ggtctgggtg gtgtcgtagc aggcggccca cagcatgcgc atgacctggg cgagctcggg
      181 ttcgtggcgg ctggccacca ggatctcgag cagcgccgtg gtatagggcg tggccatgag
      241 gttgcgccag cccacggtga tgagcttgtg caggcggtcg tcggagtctt ccaggctcag
      301 catgatcctg ccgagctcga tatagaaatg acgcaccagc ttttccgcgg cggcctcgat
      361 gagggcggcc ttgtgcggga agtggtgcac cggcgcgccg cggctgacct gggcggtggc
      421 gacgatcttg ctgaccgtgg tgccggcgta gccctcgctg accaggcact tgagggtggc
      481 ctcgaccagg cgctcgcgca tggcctcgct gcgctcggcc tgggtgcggc gggccggctt
      541 ggcgggacgg tcgcccgcgg gggcgccagc cggcccggcg ctatcggcca cggcggcggc
      601 cggctcggcg gtcggggcga tgtcggcggt cttggccatg gccaggctcc ggtggcggcc
      661 ctcctacgaa gggcaatatc gaagggcgaa ttcaataaaa acaactggtt agcttgtcgg
      721 gcggggtgtc cgggccggcc gtgggcggga ttatgcacat cggcgtccgc ttgacacccc
      781 ttccgttcgc ttgctatcaa ttaaacaagt tggacgtacg taatgtaaat attcggcccc
      841 gtccttccat ccgacctttg taggacctta tccccggagt tcccgatgag cgcagtacac
      901 attccccctc atgtcgcccc gatgatcccg cgcaccctgt tcaacgcgga ccacgaggcc
      961 ttccgcgata ccgcgcgccg cttcttcgag accgagatcg cgccgttcca cgagaagtgg
     1021 gaagagcagc agcacctgga ccgcgtgctc tggaacaagg ccggcgagct tggcctgctc
     1081 tgctgcacca tgcccgagga atacggcgga tcgggcgtgg accgcctgta ctcgatgatc
     1141 ctgatggaag aacaggcccg cgtcggcgac tccgcctccg gcttctccct gcattccgac
     1201 atcgtcgcca actacatcaa caacttcggc aacgaggagc agaagaagac ctggctgccg
     1261 aagatggcct ccggcgaagc cgtgggcgcc atcgccatga ccgagccggg caccggctcc
     1321 gacctgcagt ccatccgcac caccgcggtg gccgacggcg acgactacat cgtcaatggc
     1381 tccaagatct tcatcaccaa cggctacctc tgcgacgtcg ccgtggtggc ggtgaagacc
     1441 ggcgacatca atggcggcgc gcagagcgtg tcgctgctga tcatcgaggc caaccgcccc
     1501 ggcttcagca agggcaagcc gctgaagaag gtcggcatga agggccagga caccgccgag
     1561 ctcttcttcg acaacgtgcg cgtgcccaag agcaacctgc tcggcatgga aggcatgggc
     1621 ttcatcatgc tgatgaagga actggcctgg gaacgcatga tgatcggcat catgtgcgcc
     1681 gccggcgccg agttcgcgct cgacaccacc gtcgagtacg tgaagcagcg caaggtcttc
     1741 ggcaagcccg tcaacgccta ccagtacacc cgcttcaagc tggccgagat gcgctcggaa
     1801 gtgcagatcg cccgcgtctt cgtggaccgc tgcatggagc aggtgctgaa gaacgagctc
     1861 agcgtggagg ccgcctgcgc cgccaagtac tgggtgtccg acctgctcag caaggtcgtc
     1921 gacgagtgcg tgcagctgca cggcggctac ggcttcatgc tggagtaccc gatcgcgcgc
     1981 gcctacatcg acacccgcgc caaccgcatc tacggcggca ccaacgagat catgaaggaa
     2041 ctgatctcgc gctccatctg agcgcaatga caagggcggc ccgcaaggcc gccctttttc
     2101 catgcggtgg gcggcgactg tgctgtgccc tgtgcgggcc atggaacgac ccctgcgcct
     2161 tccggagcgc ccgccttcgc cgtcgtctgg ccattgccgc ggttatggcc gccatttcgc
     2221 atcggcaatt gccccggggc gcacgcgtca gtccgcggcg tgtgcgcgac ccgg
//
LOCUS       DASUYC010001147         2425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1590109, whole genome shotgun sequence.
ACCESSION   DASUYC010001147 DASUYC010000000
VERSION     DASUYC010001147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590109"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /locus_tag="VF276_18725"
     CDS             complement(<1..1173)
                     /locus_tag="VF276_18725"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX6608942.1"
                     /translation="MADRMAGRVLNGRYELQGILGGGGMALVYRARDRVLGRTVAVKI
                     LREQYASDPLFQQRFTREAQAAGALSHPNIASVYDVGQDGDCPYIVMEYVPGVSLRDL
                     IAREGTIPVDQAVEIAAQILSALEYAHRNGLVHRDIKPGNVLITPQGTVKVVDFGIAK
                     GASDLSLTGAGMALGTATYFSPEQARGEGARVQSDLYSVGVTLYEMLTGRPPFEADND
                     VGMAFKHINEPPPPPRQLNPMIPPQLERIVLRALAKNPAARFTSAAEMEQALRNYAAF
                     GAQATAAVPQVPRPAPAVAVVPSVAPRPAPAVAPPAPPPATLQSYRSGGLSSSSCLTW
                     VLGAVALLLLLGGTVFVLNGGLPLGFGATPTPNLNGQPTFIAPTQQRDTPVPTATGV"
     gene            complement(1244..>2425)
                     /locus_tag="VF276_18730"
     CDS             complement(1244..>2425)
                     /locus_tag="VF276_18730"
                     /inference="COORDINATES: protein motif:HMM:NF024876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HEX6608943.1"
                     /translation="RITRAVVSYNYTGEPTTGDVSATLTPGASVTVQARLNTRTHYMP
                     PGVDISYYWTIEDDAGRHFDTPPQVLRYEDKRYAWQVAEDKAHNLRVHWYKGGASFGQ
                     ALRKTAVAGLDRLQAGFGLTLTLPVEVWVYPDDASFTSALPPNLPEWVGGKAFVQNGV
                     VAALIDPAFDADHEIRRIVPHELSHLVVYQATRNPYNTPPAWLDEGLAIYNQEVQDSE
                     MDAQLKAAAEHGKLLRLRTLQGSFPADPDAATLSYAESASVVRFMIDHYGAAKVGAVL
                     AAFRDGVTYDEALQAGVGTDTDGVDSAWRAALPYKQPRVAATASPVPAQSQPAGATPG
                     VPATAAPAAATAAPAATATAASASLPAEGSNLNTILLVAAACVLVAVGTIGLLAVRRR
                     G"
BASE COUNT          358 a          856 c          843 g          368 t
ORIGIN      
        1 cacgccggtc gcggtcggca cgggcgtgtc ccgctgttgc gtcggcgcga taaacgtggg
       61 ctgcccgttc aggttcggtg tcggggtcgc gccaaagccg agcggcaggc cgccgttgag
      121 cacgaacacg gtgccgccca gcaacagcag cagcgccacc gcgcccagca cccaggtcag
      181 gcaggacgaa ctgctcaggc caccgctgcg atagctctgc aacgtcgccg ggggcggcgc
      241 gggcggggcg accgccgggg cgggccgcgg cgcgaccgaa ggcaccacgg ccacggcggg
      301 cgccgggcgc ggcacctggg gcacggcggc ggtggcctgt gcgccgaaag ccgcgtagtt
      361 acgtagcgcc tgctccatct ccgccgccga ggtaaaccgc gccgccgggt tcttcgccag
      421 ggcgcgcagc acgatacgct ccagttgcgg cgggatcatc gggttcaact ggcgcggcgg
      481 cgggggcggc tcgttgatgt gcttgaaggc catgcccacg tcgttgtccg cctcgaacgg
      541 cgggcgcccg gtcagcatct cgtagagcgt cacgccgacc gagtagaggt cggactgcac
      601 gcgcgcccct tcgccgcggg cctgctccgg cgagaagtag gtggcggtgc ccagggccat
      661 gcccgcgccg gtcagcgaca gatcgctggc gcccttggcg atgccgaagt ccactacctt
      721 gacggtgccc tgcggcgtga tcagcacgtt gcccggcttg atgtcgcggt ggaccagccc
      781 gttgcggtgg gcatattcga gtgcggacag gatctgcgcg gcgatctcca ccgcctggtc
      841 caccgggatg gtgccctcgc gggcgatcag gtcgcgcaac gagacgcccg gcacgtactc
      901 catgacgata taggggcagt cgccatcctg gccgacatca tagaccgacg cgatattggg
      961 gtgcgataag gccccggcgg cctgcgcctc gcgggtgaac cgctgctgga acagcgggtc
     1021 gctggcgtac tgctcgcgca ggattttgac ggccacggtg cggcccagca cgcggtcgcg
     1081 ggcgcgatag acgagggcca tgccgccccc acccaggatg ccctgcaact cgtagcgtcc
     1141 attcagcacc cgacccgcca tacggtcggc catgggtagt tctcctcttg ctcaaaaacc
     1201 gcagtcgcgg gccgccgccc gccgctgtaa ttctacgata ttatcagccg cgccgtcgca
     1261 cggccagcag cccgatcgtg cccaccgcca ccagcacgca cgccgccgcc acgagcaaaa
     1321 tcgtgttcag gttgctgccc tccgccggca aggatgcgga ggccgccgta gcggtcgccg
     1381 ccggcgcggc agtggcggca gccggcgccg ccgtggccgg cacgccgggc gttgcgccgg
     1441 caggctgcga ttgcgccggg acggggctgg ccgtggcggc cactcgcggc tgcttgtagg
     1501 gcagcgcggc ccgccaggcg ctgtccaccc cgtccgtgtc tgtcccaacg ccggcctgca
     1561 acgcctcgtc ataggtcacg ccgtcgcgga aggcggccag cacggcgccc accttggccg
     1621 ccccgtaatg gtcgatcata aaacggacga cgctcgcgct ctcggcgtag gagagtgtcg
     1681 ccgcgtccgg gtccgccggg aaggacccct gcaacgtgcg cagccgcagc agcttaccgt
     1741 gttcggcggc ggcttttagc tgcgcatcca tctcgctgtc ctggacctcc tggttgtaaa
     1801 tcgccaggcc ctcgtccagc caggcgggcg gcgtgttgta cgggttgcgc gtcgcctggt
     1861 aaaccaccag gtggctcaac tcgtgcggca caatgcgccg gatctcgtgg tcggcgtcaa
     1921 aggccgggtc gatcagggcc gccaccaccc cgttctgcac gaacgccttg ccgcccaccc
     1981 actccggcag attgggcggc agcgccgagg taaaactggc gtcgtcggga tagacccaga
     2041 cctcgaccgg cagggtcagc gtgaggccga agcccgcttg cagccggtcc agcccggcca
     2101 ccgccgtctt gcgcagcgcc tggccgaagc tcgcgccgcc cttgtaccaa tggacgcgca
     2161 ggttatgcgc cttgtcctcg gccacctgcc aggcgtagcg cttatcttca taacgcagca
     2221 cctgcggcgg cgtgtcgaaa tgccggcccg cgtcgtcctc gatggtccaa taatatgaaa
     2281 tgtccacgcc gggcggcatg taatgggtgc gcgtattgag ccgcgcctgc accgtcacgc
     2341 tggcgccggg cgtgagcgtg gcgctcacgt cgcccgtggt cggctcgccc gtatagttgt
     2401 acgagaccac ggcgcgggta atgcg
//
LOCUS       DASUYC010001148         2877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_434600,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001148 DASUYC010000000
VERSION     DASUYC010001148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434600"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..169
                     /locus_tag="VF276_18735"
     CDS             5..169
                     /locus_tag="VF276_18735"
                     /inference="COORDINATES: protein motif:HMM:NF012687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608944.1"
                     /translation="MVGNALITAHFDLIRGTNDHHAIEALFKAFALALHQATRLSGLA
                     GAIPSTKGVL"
     gene            171..845
                     /gene="hisH"
                     /locus_tag="VF276_18740"
     CDS             171..845
                     /gene="hisH"
                     /locus_tag="VF276_18740"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011244777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX6608945.1"
                     /translation="MAKTIAIVDYGAGNLRSIWKALARVATESSADGTGPAVVVTMDP
                     AVVAAADAVVLPGVGAAGATMRHLERHGLIAPVHEAAQSGRPFLGVCLGLQLLFEAHE
                     EDAAPGLGVLPGRARRLPGGLKVPHMGWNTVRPTSAATPLLASPTSQDESPMGGDLYF
                     YFVHSYYVEPAPELAGAIAGTTAYGLQFCSLLAYRNIWATQFHPEKSGTNGLRLLANF
                     VREVAA"
     gene            845..1606
                     /locus_tag="VF276_18745"
     CDS             845..1606
                     /locus_tag="VF276_18745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEX6608946.1"
                     /translation="MEILPSIDIKDGRNVRLYQGDYDQVTTFPDSVLEAGRRRQAEGA
                     RWLHVVDLDGARAGHPVNTALILDLRRALDLRIEVGGGLRTVADVDQLLDAGIDRVQL
                     GTAALDDPALLDHVLARDPAALSVGLDARDGIVRTGGWLLSSGRRAEEVARALAARGV
                     RCFSHTDIAHDGAMGGPNYAALAAMQAALQTGDRATRPLLIASGGVSSLAHVRRLRDM
                     GLDGVIIGRALYTGAIALLDALRVAAGAEENANAG"
     gene            1596..2387
                     /gene="hisF"
                     /locus_tag="VF276_18750"
     CDS             1596..2387
                     /gene="hisF"
                     /locus_tag="VF276_18750"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEX6608947.1"
                     /translation="MPADTAILNGLTRRIIPCLDVTAGRVVKGVHFVDLRDAGDPVEL
                     AAFYDRAGADELVFLDITASSDSRRTVIDLVERTAAQVFIPLTVGGGVRAVEDIRALL
                     GAGADKVSLNTAAVRDPALISRGAEAFGSQCIVVAIDARHDPAAPSGWGVFTHGGRTP
                     TGLDALAWAREAVERGAGEILLTSMDRDGTKDGYDLPLTQAVAAAVPVPVIASGGAGT
                     LDHFRAVLAPGGADAALAASIFHFGEYSVAQVKRYLADHGVAVRL"
     gene            2384..2800
                     /gene="hisI"
                     /locus_tag="VF276_18755"
     CDS             2384..2800
                     /gene="hisI"
                     /locus_tag="VF276_18755"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF000768.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEX6608948.1"
                     /translation="MSEPSQEAQAPGLVDVVCFGADGLVPVVVQAGGDRQVLLVGSMN
                     REALARTLTSGRLWLWSRSRGQLWLKGETSGHYQQVDAVYINCELNSLLIQVTSLGPI
                     CHEGYATCFYRRAHATGELETVATREFDPSTVYPAP"
BASE COUNT          407 a          992 c         1031 g          447 t
ORIGIN      
        1 cgccctggtc ggcaacgcgc tgatcacggc ccatttcgac ctgatccgcg gcaccaacga
       61 ccaccacgcc atcgaggcgc tgttcaaggc gttcgcgctg gcgctgcacc aggccacgcg
      121 cctgagcggt ctggccggcg cgatcccgtc gaccaagggg gtgctgtgag atggcgaaaa
      181 ccatcgcgat tgtcgactac ggcgcgggca acctgcgcag catctggaag gccctggccc
      241 gcgtcgccac cgagtcgagc gcggacggca ccggcccggc ggtcgtcgtg accatggacc
      301 cggcggtcgt ggccgccgcc gatgcggtgg tgctgcccgg tgtgggcgcg gcgggcgcga
      361 cgatgcgcca cctggagcgc cacgggctga ttgcaccggt gcacgaggcg gcccagagcg
      421 ggcggccctt cctgggcgtc tgcctgggcc tgcaactgct gttcgaggca cacgaagaag
      481 acgcggcgcc ggggttgggc gtcttgccgg ggcgcgcgcg ccgcctgccg ggcggcctga
      541 aggtgccgca catgggctgg aacaccgtgc ggcccacatc cgccgccact cccctgctcg
      601 cttcgcccac atcgcaggac gagagtccaa tgggcggcga cctatacttt tacttcgtgc
      661 attcatatta tgtcgagccg gcccctgaac tggccggggc catcgccggg accacggcct
      721 atgggctgca attttgcagc ctgctggcct accgcaacat ctgggcaacg cagtttcacc
      781 cggagaaaag cggcaccaac ggcctgcgcc tgctcgccaa cttcgtgcgg gaggtggcgg
      841 cgtgatggag atcctgcctt cgattgacat caaggatggg cgcaacgtgc ggctctatca
      901 gggcgactac gaccaggtga ccaccttccc cgactcggtg ctggaagccg ggcggcgccg
      961 gcaagccgag ggcgcgcgct ggttgcacgt ggtcgatctc gacggggcgc gggctgggca
     1021 cccggtgaac accgccctga tcctggacct gcgccgggcg ctggacctgc gtatcgaggt
     1081 gggcggcggc ctgcgcaccg tggccgatgt ggaccagttg ctggacgccg ggatcgaccg
     1141 ggtgcaactc ggaaccgccg ccctggacga cccggccttg ctggaccatg tgctggcccg
     1201 cgaccccgcc gcgctgtccg tggggctgga cgctcgcgac ggcatcgtgc ggaccggcgg
     1261 ctggctccta tcgagcgggc ggcgggccga ggaggtcgcg cgggcgctgg cggcgcgcgg
     1321 ggtgcgctgc ttcagccaca cggacatcgc ccacgacggc gccatgggcg gcccgaacta
     1381 cgctgcgctg gcggccatgc aggcggcctt gcagaccggt gatcgggcaa cccgccccct
     1441 actgatcgcg tccggcggcg tctcgtccct cgcgcatgtg cgccggctgc gcgatatggg
     1501 gctggacggg gtgattatcg ggcgggcact ctataccggc gccatcgcct tgctcgacgc
     1561 gctacgtgtt gccgccggtg ccgaggagaa cgccaatgcc ggctgatacc gctatcctga
     1621 acggcctcac gcgccgcatc atcccctgcc tcgacgtgac cgcggggcgg gtggtcaaag
     1681 gcgtccactt cgtggatctg cgcgatgccg gcgacccggt ggaactggcc gccttctacg
     1741 atcgcgcggg cgccgacgaa ctcgttttcc tcgacatcac cgcgtcgagc gacagccgcc
     1801 gcacggtcat cgacctggtg gagcgcaccg ccgcccaggt cttcatcccg ctgacggtgg
     1861 gcgggggcgt gcgggccgtt gaggacatcc gcgcgctgct gggcgcgggc gcggacaagg
     1921 tctcgctcaa caccgccgcc gtgcgcgacc cggcgctgat cagccggggg gccgaggcct
     1981 tcggcagcca gtgcatcgtc gtcgccatcg acgcccggca cgacccggcg gcgccgtcgg
     2041 gctggggcgt gttcacgcac ggcgggcgca cacccaccgg cctcgatgcc ctggcctggg
     2101 cacgtgaggc ggtggaacgc ggggcgggcg agatcttgct gaccagcatg gaccgcgacg
     2161 gcaccaagga tggttacgat ctgccgctga cccaggcagt ggccgccgcc gtgcccgtcc
     2221 ccgtgatcgc ctcgggcggc gcgggcacgc tggaccactt tcgggcggtg ctggcgccgg
     2281 gcggggcgga cgcggcgctc gccgcctcga tcttccactt cggcgagtac agcgtggccc
     2341 aggtgaagcg ctacctggcc gaccacggcg tggcggtgcg gctatgagcg agccgtccca
     2401 ggaagcgcag gcgcccggcc tggtcgatgt ggtgtgcttc ggcgccgatg ggctggtgcc
     2461 agtagtggtg caggccggcg gcgaccggca ggtgttgctg gtgggcagca tgaaccggga
     2521 ggcgctggcg cgcacgctga ccagcgggcg gctctggctc tggagccgtt cgcgcgggca
     2581 gctctggctc aaaggtgaga catcgggcca ctaccagcag gtcgacgccg tctacatcaa
     2641 ctgcgagctg aacagcctgc tgatccaggt aacatcactc ggcccgatct gccacgaggg
     2701 ctacgcgacc tgcttctacc gccgcgccca tgctaccggc gagcttgaga cggtcgccac
     2761 gcgcgagttc gatcccagca cggtctaccc tgccccctga tttgtcactc tgagtagtct
     2821 gtcacatggt ttttgtcact ctgagcgcaa cgaagagtct cgtctttcta gctacga
//
LOCUS       DASUYC010001149         5308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1129401, whole genome shotgun sequence.
ACCESSION   DASUYC010001149 DASUYC010000000
VERSION     DASUYC010001149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5308
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129401"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VF276_18760"
     CDS             <1..464
                     /locus_tag="VF276_18760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608949.1"
                     /translation="AVRHADPVWAEALLGALPALRTDDTLNNLIALLPAERWEALVLD
                     SLRAHAGPLRMNHPAGVLLQARPALWSPALSRAVLDHLLPAIGAGKLPPHWDVRQLLQ
                     LAACCLAPAVLPEAVRRWPVAPDSGSPWTAAGDEFLGLVEFRDTMLKEISR"
     gene            461..1543
                     /locus_tag="VF276_18765"
     CDS             461..1543
                     /locus_tag="VF276_18765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6608950.1"
                     /translation="MTIVLREHAEQQYAEELAELARADTRTRPPNWNLSPWAVTSYLL
                     GGKLDNGFVVSPKYIGSRRLIEIAVATLTTDRALLLLGVPGTAKTWVAEHLSAAIAGD
                     STLLVQGTAGTSEESIRYGWNYARLLAEGPSLGALVPSPLMVAMQKGKIARVEELTRI
                     PADVQDSLITILSEKTLPSPELGEEVQAVKGFNVLATANNRDRGVNELSSALKRRFNT
                     VILPVPASADEEVEIVERRVASLGRALELPAEKPALEEIRRVVTIFRELRDGVTADGK
                     TKLKSPSGTLSTAEAISVVSQGLALAGHFGDGRLRAGDVAAGLVGAVVKDPVQDRVVW
                     LEYLETVVKERGGWKDLYRACREVSE"
     gene            1540..2559
                     /locus_tag="VF276_18770"
     CDS             1540..2559
                     /locus_tag="VF276_18770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018895029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608951.1"
                     /translation="MSAREQPGPVADLADLQCWDVLAPERREAVARAVAARLPGSFRF
                     AGMATHTLGDQTHTLAFFDRDGARFALIPGGEVTLGYDPATFVPSPGQVAAMDEMREY
                     FAEDDDDATVDLPVYLAQLASGEYKDRFLTPVRRATIAPFLLETQATEAGVFPVPAEE
                     YLTAAQIASFRRQPGIQAEMGVSGQAIRVRSGRDGAVRVVVNRHPTHGEVTAQLARTG
                     FRLPSSDEWEYACGAGARTLFRWGDDCPLRRLPILDGEDEPDSTWDLHARPNAFGLHF
                     SGNPYDWEICTEPGLMRGGDGGVAICGGEGPVAAWTALATAFASRWEHDKLFGAHMRR
                     AYSLL"
     gene            2572..4908
                     /locus_tag="VF276_18775"
     CDS             2572..4908
                     /locus_tag="VF276_18775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012627861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HEX6608952.1"
                     /translation="MAEAAKTRSGNNGVHVFGIRHHGPGSARSLRRALEELRPDVILV
                     EGPPDAAEVLPLLAHAEMEPPVALLIYRPDEPRRAVYYPFAGFSPEWQAIHYGLTGGI
                     PVRFMDLPQAHRLAGPAGEEATEAPPPEPPAEPFTEDAPPAEAASPVDEEPPAPDPWA
                     DPLGWLAQAAGYSDGERWWDHLVESRRDSAAVFDAILEGMTALRAALPPRDDPEEAQR
                     EAYMRQTIRAAQREGYGRIAVVCGALHAPALVDPPPAKADAALLAGLSRVKVAATWVP
                     WTHGRLLMAGGYGAGIESPGWYEHLWQTGGDSATSEQVAVRWMTRVARLLRDEDLDAS
                     AAHVIEAVRLAHALAALRDRPVPGLPELNEATRTVLCFGDDLPMGLIHDKLIVGERLG
                     AVPDDTPVVPLQADLQREQRRLRLPAEATQRQLDLDLRKPVDLDRSRLLHRLALLGVP
                     WGRLNRVAGKKGTFHELWQTQWDPAFAVALIEAGIWGNTILDAATAFARAAADRAPDL
                     PALTSLVEQALLADLPDAMTHLMERLQAEAARASDVAHLMAALPPLASVLRYGNVRGT
                     DAGMVGTVVDGLVARICIGLPGACASLNDEAADAMFGHIMDVHGAVALLQNPDQRAAW
                     QDTLRRLADQAGVHGLVAGRCCRILLDAEALSAEDVARRLGLALSRAANPPAAAAWVE
                     GFLRGSGLLLLHDEALWQVLDTWVTSLPADTFTALLPLLRRTFATFPAAERRKMGEKV
                     RSGPARPAVATTAEDFDAARAETVLPLVARLLGLTPAG"
     gene            4973..>5308
                     /locus_tag="VF276_18780"
     CDS             4973..>5308
                     /locus_tag="VF276_18780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608953.1"
                     /translation="MADSALTKKLGLKPGRRALILNAPDGYRALLDPLPQGVDLTTTP
                     DGTFDFVQLFAKNKADLAEYAPAAMAAVNPGGMLWISFPKKTSKIQTDISRDTGWDVI
                     QQAGWQGVFL"
BASE COUNT          775 a         1866 c         1883 g          784 t
ORIGIN      
        1 ccgcggtgcg ccacgccgat cccgtgtggg ccgaggcgct gctgggcgcg ctaccggcct
       61 tgcgcaccga cgatacgctg aacaacctca tcgccctgct gcccgcggag cgctgggagg
      121 cgctggtgct ggatagcctg cgcgctcatg ccgggccgct gcgcatgaac cacccggcgg
      181 gggtgctgct gcaagcgcgc ccggcgctct ggagtcccgc cctgagccgc gccgtgctcg
      241 accatctctt gccggccatc ggcgccggga agttgccgcc gcactgggac gtgcggcaac
      301 tattgcaatt agcggcctgt tgcctggcgc ccgcggtttt gccggaggcg gtgcgccgct
      361 ggccggtcgc gcccgactcc ggctcgccct ggaccgcggc gggcgatgaa tttctcggcc
      421 tggtcgaatt ccgcgatacc atgctcaagg agatctcccg atgaccattg tcctgcgcga
      481 acacgccgaa cagcaatatg ccgaagagtt ggcggaactg gcccgcgccg acacgcgcac
      541 ccggcccccc aactggaacc tgtcgccctg ggctgtcacg agctatctgc tgggtggcaa
      601 gctcgacaac ggcttcgtcg tctccccgaa atacatcggc agccgccgcc tgatcgagat
      661 cgccgtcgcc acgctgacca cggaccgcgc gctgctgctg ctgggtgtgc ccggcacggc
      721 caagacctgg gtggccgagc atctgtcggc ggccatcgcc ggggactcca cgctgctggt
      781 gcagggcacg gcgggcacca gcgaggagtc gatccgctac ggctggaact atgcccgcct
      841 gctggccgag ggaccgtcgc tcggcgcgct ggtgcccagc ccgctgatgg tcgccatgca
      901 gaagggcaag atcgcccgcg tggaagagct gacgcgcatc cccgccgacg tacaggactc
      961 gctgattacc atcctgtcgg aaaagacgct gcccagcccg gagttgggcg aagaagtgca
     1021 ggcggtcaaa ggcttcaacg tcctcgccac ggccaacaat cgcgaccggg gcgtgaacga
     1081 actgtccagc gccttgaagc gccgcttcaa cacggtcatc ctgccggtgc ccgccagcgc
     1141 cgacgaagag gtggagatcg tcgagcgccg ggtggcgagc ctgggccggg cgctggaact
     1201 gcccgccgag aagcccgcgc tggaggagat ccgccgcgtt gtgaccatct tccgcgaact
     1261 ccgcgacggc gtcaccgcag acggcaagac caagctcaag tcacccagcg gcacgctcag
     1321 cacggcggag gccatctcgg tggtgagcca gggcctggcg ctggccgggc acttcggcga
     1381 cgggcggctg cgcgccgggg acgtagcggc gggcctggtc ggcgccgtcg tgaaagaccc
     1441 ggtgcaggac cgcgtggtct ggctcgaata cctggaaacc gtcgtcaagg agcgcggcgg
     1501 ctggaaggac ctctaccgcg cctgccgcga ggtcagcgaa tgagcgcgcg cgagcaaccg
     1561 gggccggtcg ccgatcttgc cgatctccag tgctgggacg tgctcgcccc ggaacggcga
     1621 gaagccgtgg cgcgggccgt ggcagcgcgc ttgccagggt ccttccggtt tgccgggatg
     1681 gcgactcata ccctgggcga ccagacgcat actctggctt tcttcgaccg tgacggcgcc
     1741 cggttcgcgc ttatccctgg cggtgaggtt acgctgggct atgacccggc gaccttcgtg
     1801 ccctcgcccg gccaggtcgc ggccatggat gagatgcgcg agtactttgc cgaagacgac
     1861 gacgacgcca cagtggactt acccgtttac cttgcccagt tggcgagcgg ggaatacaaa
     1921 gatcgcttcc tcacgccggt tcggcgcgcg acaatcgcgc ctttcctgct cgaaactcag
     1981 gctacggagg ccggggtgtt tcccgtgccc gccgaggagt acctgacggc ggcccagatt
     2041 gcctcgttcc ggcgccagcc gggcatacag gcggagatgg gggtctccgg tcaggcgatc
     2101 cgggtgcgat cgggccgcga cggcgccgtc cgcgtggttg tcaatcgcca ccccacccac
     2161 ggggaggtga cggcgcaact ggcgcgtacc ggcttccggc tgccgtcgtc cgacgagtgg
     2221 gagtacgcct gcggcgcggg cgcacgcacg ttgttccgct ggggcgacga ttgccccctg
     2281 cggcgcctgc ccatcctgga tggtgaagac gagccggaca gcacctggga tctgcatgcg
     2341 cgacccaatg ccttcggcct gcacttttcc ggcaacccct acgactggga aatctgcacc
     2401 gaaccgggcc tcatgcgcgg cggcgacggc ggggtggcga tctgtggcgg ggaaggcccc
     2461 gtggccgcct ggacggcgct ggcgacagca ttcgcctcgc ggtgggagca cgacaaactc
     2521 tttggcgcgc atatgcggcg cgcatattcc ttactctgag cgggaggcta catggcagag
     2581 gcggcaaaaa cccggtccgg caacaacggg gtgcatgtct tcggcatccg ccaccacggc
     2641 cccggctcgg cacgcagcct gcggcgggcg ctggaggagt tgcggcccga cgtgatcctg
     2701 gtggaggggc cgcctgacgc cgccgaggtg ttgccgctgc tggcgcatgc cgagatggag
     2761 ccgccggtgg ccctgctgat ctaccggccc gatgagccgc gccgcgccgt ctattatccg
     2821 ttcgccggct tctcgccgga gtggcaggcc atccactacg ggctgaccgg cggcatcccc
     2881 gtgcgtttca tggacctgcc gcaggcccac cggctggccg gcccggcagg ggaggaagcc
     2941 accgaagcgc cgccgcccga accgcccgcc gagccgttca ccgaggacgc gccgcccgcc
     3001 gaggccgcca gtcctgttga tgaggagccg cccgcgcccg atccctgggc cgacccgctg
     3061 ggctggctgg cccaggccgc gggctacagc gacggcgagc gctggtggga ccacctggtc
     3121 gagagccgcc gcgacagcgc cgcggtcttc gacgcgatcc tggagggcat gacggcgctg
     3181 cgcgcggccc tgcccccgcg ggacgaccca gaggaagcgc agcgcgaagc ctacatgcgc
     3241 cagacgatcc gcgctgccca gcgcgagggc tacggacgca tcgccgtcgt ctgcggggcg
     3301 ctgcacgcgc ccgcgctggt cgatccgccg cccgcaaagg ccgacgccgc gctgctggcc
     3361 gggctgtcca gggtcaaagt ggccgcaacc tgggtgccct ggacccatgg ccggctgctc
     3421 atggccgggg gctacggcgc gggcatcgag tcgcccggct ggtacgagca cctgtggcag
     3481 accggcggcg acagcgccac gagcgaacag gtggcggtgc gctggatgac ccgcgtggcc
     3541 cggctgctgc gcgacgagga cctggacgca tccgccgccc atgtgatcga ggcggtgcgc
     3601 ctggcccacg ccctggccgc cctgcgcgac cggcccgtgc cgggcttgcc cgaactgaac
     3661 gaggccacgc gcaccgtgct ctgcttcggc gacgacttgc cgatgggcct gatccacgac
     3721 aagctaatcg tgggcgagcg gttgggcgca gttcccgacg acacgccggt ggtgcccttg
     3781 caggccgatt tgcagcgcga gcagcgccgc ctgcgcctgc ccgccgaggc gacccagcgc
     3841 cagctcgacc tggacctgcg caagccggtg gatctggatc gcagccgcct gctgcaccgg
     3901 ctggcgctgc tcggggtgcc ctggggccgc ttgaaccgcg tggcgggcaa gaagggcacg
     3961 ttccacgaac tctggcagac gcagtgggac ccggctttcg ccgtggcgct gatcgaggcg
     4021 ggcatctggg gcaacacgat cctcgatgcg gccacggcct tcgcccgcgc cgccgccgat
     4081 cgggcgccgg acctgcccgc gctgaccagc ctggtcgagc aggcgctgct ggccgatctg
     4141 ccggacgcca tgacccacct gatggagcgc ttgcaagccg aggcggcgcg ggccagcgat
     4201 gtcgcccacc tgatggccgc tctgccgccg ctggcgagcg tgctgcgcta cggcaacgtg
     4261 cgcggcaccg acgcgggcat ggtcggcaca gtggtggatg gcctggtcgc ccgcatctgc
     4321 atcgggctgc ccggcgcctg cgcctcgctc aacgacgaag ccgcggatgc gatgttcggg
     4381 cacatcatgg atgtccacgg cgcggtggcg ctgttgcaaa accccgacca gcgcgccgcc
     4441 tggcaggata cgctgcgccg gttggccgac caggcgggcg tgcatggact ggtggccgga
     4501 cgctgctgcc gcatcttgct cgacgccgaa gcgctatccg ccgaggacgt ggcccggcgg
     4561 ctggggctgg ccctgtcgcg cgcggccaac ccgcccgccg ccgcggcctg ggtcgagggc
     4621 ttcttgcgcg gcagcggcct gttgctcttg cacgacgagg cgctctggca ggtgctcgac
     4681 acctgggtca cgagcctgcc cgccgatacg ttcacggcgc tgctgccgct gttgcgccgc
     4741 actttcgcca ccttccccgc cgccgagcgc cgcaagatgg gcgagaaggt gcgcagcggc
     4801 cccgcccgcc ccgccgtggc gacgaccgcc gaggacttcg acgccgcccg cgccgagacg
     4861 gtgttgccgc tggtggcccg gctgctgggc ctgacgcccg cgggttgaac gggtgtggta
     4921 tcctggggaa gacgtgtctt tggcctgctg cgtgtgaatg gagatgcccc ctatggcgga
     4981 tagtgccctg acgaaaaagc tcggcctgaa gccgggccgg cgcgctctga tcctcaacgc
     5041 cccggacggc taccgggcct tgctcgatcc cttgccccaa ggcgtggacc tgaccactac
     5101 cccggacggc accttcgact tcgtgcagtt gttcgcgaag aataaagccg acctggcgga
     5161 atacgccccg gcggcgatgg cggcggtcaa tccgggcggg atgctctgga tctcgtttcc
     5221 caagaaaacc tcgaagatcc agaccgacat cagccgcgat accggctggg acgtgatcca
     5281 gcaagcggga tggcaagggg tctttctg
//
LOCUS       DASUYC010001150         3480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1129530, whole genome shotgun sequence.
ACCESSION   DASUYC010001150 DASUYC010000000
VERSION     DASUYC010001150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129530"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            527..1618
                     /locus_tag="VF276_18785"
     CDS             527..1618
                     /locus_tag="VF276_18785"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6608954.1"
                     /translation="MEVIDQARSRLARGDGAGAQALLDKALQGYQAAGDRAGGAAVLH
                     EMAVQARTRGDLAAAEPPLRQAIQLRLSLGDHAGWVASVFELGLLERARGQSALATDI
                     FARALYSARQLNDYPHIAACLHYLALIHGEAGDLDTAETLLNEALEVRGTIDDRTGQS
                     ATLHQLGRLREGQHDLFAALDYYKRSLAFAEQAGDAAGQLANLTQLGAVHQARNRPAE
                     AERCYRAALDLARREGRPDLIAANLRHLGEAAYNRGDYAAAFEALWQAHTLQPGDTPL
                     KWMHPIYLSAGPDVFLAQARRLGVDPALLDQVARYVPPSDGGGRPTLAQLVALRLRVG
                     PQQFAVLAQQSQIPADVVAKVESAAQSLV"
     gene            2210..>3480
                     /locus_tag="VF276_18790"
     CDS             2210..>3480
                     /locus_tag="VF276_18790"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6608955.1"
                     /translation="METQRSTLESLGQRLRQARLERHMSQEKLAQPEFTKSYVSAVER
                     GKARPSLKALELMARRLEIPMNELLHAAPPAEDLPNLPALQADLSYELDAAQMLINTN
                     QADEALRRINAAEQTAGPHLVEFDEDTRYRLYYLRARAYLRLAEPASARHELQQAMSL
                     AQQRGAEEDVERVRNDIGGAFFEQDMPGLALEQHKQCLHAIHTGVVKDLNLRLSIYSN
                     IANDYWALNDVEQAIGVYKESLELLEDTNNLERQSGIYWGLSLAYRAAGDLDRAKLYA
                     GRALSIFEAADNLVSAAKMSVNLADILTAREEYDEAERRLDRAQELLLPTGNQLFLST
                     VYEHYALLDLARGRLDEAADWARRSRELSESVIGETQNADAMTQANTMRTAARALRAA
                     GLVEEQRGDTRAADDLFGRALDLLSRVENDET"
BASE COUNT          599 a         1266 c         1097 g          518 t
ORIGIN      
        1 cggctacgcg gtgcgctacg aggagttcga cggcccgcac accgtcccac ccgccatcgc
       61 ccgcgccgcg gtgcagtggt tcaccccggt cgccgcctag cccgcccggc gtccctgtca
      121 ctctgagcgc agcgaagagt ctccgcgcgg ccccaacgag acggagattc ttcgctgcgc
      181 tcagaatgac gggggcgtgg ctgccgttcg cccgattacc cccggcgtgc gggaatgtcg
      241 tccgaggcgg gcgcgggcgg gcgcgctttc gataaatctg cgatacagcc ccgctacact
      301 aagggcgcag acggcgcgga tcgggcccgc catccggcac ccgaaccccg ccgcgccggc
      361 gagatagccg cgtccggccg ctacccggtt gacaagtacg gcgggcggtt ctacaatagg
      421 tcatagtagg gggtactcag cgatgatccc gagggttggc acggtgccca ggacggccac
      481 cgaaacacgg ctctacgcgc ataccccacc gcacggagaa cgcgatatgg aggtcatcga
      541 ccaggcgcgc tcccggcttg cgcgcgggga tggcgcggga gcgcaagccc ttctcgacaa
      601 agccctccag ggctatcagg cggccggcga ccgcgcgggc ggcgcggcgg tgttgcatga
      661 gatggcggtg caggcccgca cccgcggcga cctcgctgcc gccgaacccc ccctgcgcca
      721 ggccatccag ttgcgcctca gcctcggcga ccacgccggg tgggtggcga gcgtgtttga
      781 actcggcctg ctggaacgcg cccgcggcca gtccgccctc gccaccgata tcttcgcccg
      841 cgccctctac agcgcccgcc agctcaacga ctacccgcat atcgccgcct gcctgcacta
      901 cctggccctc atccacggcg aggccggcga ccttgatacc gccgagacgc tgctcaacga
      961 ggccctggag gtgcgcggta ccatcgacga ccgcaccggc cagagcgcca cgctgcacca
     1021 gttgggacgc ctgcgcgagg gccagcacga cctgttcgcc gcgctcgact actacaaacg
     1081 cagcctggcc ttcgccgaac aggcgggcga tgccgcgggc caactcgcca acctgaccca
     1141 gttgggcgcc gtccaccagg cgcgcaaccg ccccgccgaa gccgagcggt gctaccgcgc
     1201 cgcgctcgac ctggcccgcc gcgaggggcg ccccgacctc atcgccgcca acctgcgcca
     1261 cctcggcgaa gccgcctaca accgcggcga ttacgccgcc gccttcgagg cgctctggca
     1321 ggcccacacg ctgcagcccg gcgacactcc gctgaagtgg atgcacccca tctacctgtc
     1381 cgccggcccc gatgtcttcc tcgcccaggc ccggcggctc ggcgtcgacc ccgcgctgct
     1441 cgaccaggtg gcgcgctatg taccgcccag cgacggcggc gggcggccca ccctggccca
     1501 actggtggcc ctgcgcctgc gcgtcggccc gcagcagttc gccgtcctcg cccagcaatc
     1561 gcaaatcccc gccgacgtcg tcgccaaggt cgaatccgcg gcgcaaagcc tggtctgacc
     1621 gcaccccgcc ggccgtccgg cgccgcgctc agcagtctgt cacaggtatc ttgtcattct
     1681 gagcgcagcg aagaatctcg tctcgtcgcc cagactctgc gctgcgctcc gaagcgacac
     1741 gcggcggccc gccgtcgaga cgcttccctg cgctcagagt gacaagggcc ggcccgcgag
     1801 ggcggctatt gcacccggtc gcaagcgata aacgccgtcg ccgttcattc cgaatagtgt
     1861 atcgggtcgc tatattctgg aacaccatac cgcctgatcc atcgccctgc cgctcgccga
     1921 gcgcgggaaa ggcgagtgac acttgcaacc ttttcgcggt cgcctgcgtc tgatctatag
     1981 cgggccgtgc tacccgaggc atctgtgcgc gtcgcaggtg gcccgccggg gtagcgccga
     2041 tgtagtaaat tgcggccctc gggcaaacag tttctcagtt ccttaataga ttcttatcta
     2101 tctgtgacgt gtaaccgact tgttccctta ttgggagtgt gatatactgg gtttgacccg
     2161 aggtttcgcg gtcgattata gtactacaca gtagtagggg ggaactacca tggaaacaca
     2221 gcgtagcacg ctggaatcgc tgggccagcg cttgcgccag gcgcggctgg agcggcacat
     2281 gagccaggaa aaactggccc agcccgagtt taccaagagc tatgtcagcg cggtcgagcg
     2341 cggcaaggcg cggccgtcgc tgaaagcgct ggagttgatg gcgcgccggt tggagatccc
     2401 gatgaacgag ctgcttcatg ccgcgccgcc cgccgaggat ctgcccaatc tgccggcctt
     2461 gcaggccgac ctcagctatg agcttgatgc cgcgcagatg ctcatcaata ccaaccaggc
     2521 cgatgaagcc ctgcgccgca tcaacgccgc cgagcagacg gcgggcccgc acctggtcga
     2581 gtttgacgag gatacgcgct accgcctgta ctacctccgc gcccgcgcct acctgcgcct
     2641 ggccgaaccg gccagcgccc gccacgagct ccagcaggcc atgtccctgg cccagcagcg
     2701 cggcgccgag gaagatgtcg agcgcgtgcg caatgacatc ggcggcgcct tcttcgagca
     2761 ggatatgccg gggctggccc tggagcagca taaacaatgt ttgcacgcca ttcacaccgg
     2821 cgtcgtcaag gacctgaacc tgcgcctcag catctacagc aatatcgcca atgactattg
     2881 ggcgctgaac gacgtcgaac aggcgatcgg ggtctataaa gaatcgctgg aactgctcga
     2941 agataccaac aacctggagc ggcaatcggg catctactgg ggcctcagcc tggcctatcg
     3001 cgccgccggc gatctggacc gcgccaagct ctacgccggc cgcgccctga gcatcttcga
     3061 ggccgcggac aacctggtct cggcagccaa gatgagcgtc aacctggccg acatcctgac
     3121 ggctcgcgag gagtatgacg aggccgagcg gcgactcgac cgcgcccagg aactgctgct
     3181 gcccaccggc aaccagctct ttctcagcac ggtctacgag cactatgccc tgctcgacct
     3241 ggcccgcggg cggctcgatg aggcggcgga ctgggcgcgc cgcagccgcg aactgagcga
     3301 gagcgtcatc ggcgagacgc agaacgccga cgccatgacc caggccaaca cgatgcgcac
     3361 cgccgcccgc gccctgcgcg ccgccgggtt ggtcgaagag cagcgcggcg atacccgcgc
     3421 cgccgacgac ctcttcgggc gcgccctcga cctgctgtcc agggtcgaga atgacgagac
//
LOCUS       DASUYC010001151         2118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_2052080, whole genome shotgun sequence.
ACCESSION   DASUYC010001151 DASUYC010000000
VERSION     DASUYC010001151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2052080"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1439)
                     /locus_tag="VF276_18795"
     CDS             complement(<1..1439)
                     /locus_tag="VF276_18795"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX6608956.1"
                     /translation="MSGNVTRKHFARLGLSSTALAVGLLTGGTAVAQDATPPPPARSA
                     DTQANAEPGADKTIIVTGSRVARSTFDTASPVTVLDAKVIDDLNLNNVGEVIAQLPAN
                     SNFFAPNNVGLGNFNVGAQLVNLRGLNPFFGTRTLTLVDTRRVVPTTTGGGVDITLMP
                     SMLVQRTETVTGGASAIYGSDAIAGVVNIILDTKLDGFKGQADFSQTSHGDGDDLHFS
                     GAYGTGFAEDRGHVVVGVEYDNQDAIGICSEVRGWCGENYNLITNQAYLANGDPHYII
                     ARDTYLANTSLTGVLLPCVAPGGLCVGGTPFQLSTDGQSTLPFDRGQYSYLAGYGASR
                     VGGDALAVGPYDTTTLRPSVKRWTALGHVSYDLSDALSGFVELSYANTKGVNPVANGT
                     IGPYFFELVPNLYTGARIAPDNAFLSPAVAAAIGPIGADFGRNMVNVQSALNQTNNET
                     WRAVAGLNGEIGPNWSWDAYYSHGVNKNE"
     gene            1645..>2118
                     /gene="ccsA"
                     /locus_tag="VF276_18800"
     CDS             1645..>2118
                     /gene="ccsA"
                     /locus_tag="VF276_18800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX6608957.1"
                     /translation="MHAFANPARFLRIARPLTPVLLLSGLAITAAALVYGLFYAPAER
                     LQGETVRILFIHVPTAWLGMAGWSAIALASLVELVWRHPLAALAARGSAVPGAVFAAL
                     CLATGSLWGRPAWGTYWVWDGRLTSMLVLLFLYLGYIALAGAVQREGGSSRIPAIF"
BASE COUNT          314 a          663 c          748 g          393 t
ORIGIN      
        1 tgctcgttct tgttcacgcc gtgcgagtag taggcgtccc aggaccagtt cggcccgatc
       61 tcgccgttta gaccagcgac ggcgcgccag gtttcgttgt tggtctggtt gagggcggat
      121 tggacattga ccatgttgcg cccgaagtcg gccccgatcg ggccgatggc ggcggcgacg
      181 gccggtgaca ggaaggcgtt gtcgggcgcg atccgcgctc cagtgtacaa attcggaaca
      241 agctcgaaga agtacggccc gatcgtcccg ttggcgaccg ggttcacgcc cttggtgttg
      301 gcgtaggaca gctcgacgaa gccggagagg gcatcgctca ggtcgtaact cacatggccg
      361 agggccgtcc aacgcttcac cgaagggcgc agggtggtgg tgtcgtaagg ccccacggcg
      421 agagcgtcgc cgcccacccg ggaggcaccg tatccggcga ggtaagagta ttgcccccga
      481 tcgaacggca gagtcgactg gccgtccgtg gaaagctgga acggcgtccc gccgacgcac
      541 aggccgccgg gcgcgacgca gggcagcagc accccggtca ggctggtatt ggcgagataa
      601 gtgtcgcggg cgatgatgta gtggggatcg ccgttcgcga gataggcctg gttggtgatg
      661 aggttgtagt tctcaccgca ccacccgcgc acttccgaac agatcccgat ggcgtcctgg
      721 ttgtcgtact cgacgccgac gacgacatgg ccgcggtctt cggcaaaacc ggtgccgtag
      781 gcgcccgaga agtggaggtc gtcaccgtcg ccgtggctgg tctggctgaa atccgcctgg
      841 cccttgaacc cgtcgagttt ggtgtcgagg atgatgttga cgacgccggc gatggcgtcc
      901 gaaccgtaga tcgccgaggc acccccggtg accgtttccg tccgctgcac cagcatcgaa
      961 ggcatgagcg tgatgtccac gccgccgccg gtggtggtgg gcactacccg gcgggtatcc
     1021 accagcgtca gcgtgcgggt accgaagaag ggattgaggc cgcgcaggtt gaccagttgc
     1081 gcgccgacgt tgaagttgcc taggccgacg ttgttggggg cgaagaagtt ggagttcgcc
     1141 ggcaattgcg cgatcacttc gccgacgttg ttcaggttga gatcgtcgat cactttcgcg
     1201 tcgaggacgg ttaccgggct ggcggtatcg aacgtgctgc gcgcgacccg cgacccggtg
     1261 acgatgatcg tcttgtccgc gccgggctcg gcattcgcct gagtatcggc gctccgagca
     1321 ggaggtggag gggtggcgtc ctgggccacc gcggtgccgc ccgtcagcag gccgaccgcc
     1381 agtgcggtgc tgctgagacc aagacgcgcg aaatgcttac gggtcacgtt gccgctcata
     1441 ctctctctcc ctgtagagtg gggccaccgg gctccttctt gttagccggt gcaaattccg
     1501 tcacgctttt gaaaaagtca aacgaatgcg acatactcgc gggggctatc tcgacggcgt
     1561 tgccgatccg gaatgtcccg aggcaggccg cttccgcgct tgagcgcgcc cggttgctgc
     1621 tctaacgcgg cgccggcccc gatcatgcac gccttcgcca atcccgcccg cttcctgcgc
     1681 attgcccggc cgctgacgcc ggtgctgttg ttgtcgggcc tggcgatcac ggcggcggcg
     1741 ctggtctacg gcctgttcta cgcgcccgcc gagcgcctgc agggcgagac cgtgcggatc
     1801 ctcttcatcc acgtgccgac cgcctggctg ggcatggccg ggtggagcgc gatcgcgctc
     1861 gccagcctgg tcgagctggt ctggcgccat ccgttggcgg ccctggcggc gcgcggcagc
     1921 gccgtgccgg gcgcggtctt cgccgccctg tgcctggcca ccggatcgct gtggggccgg
     1981 ccggcctggg gcacgtactg ggtctgggac gggcggctga cgagcatgct ggtgctgctt
     2041 ttcctttacc tcggctacat cgcccttgcc ggggccgtgc agcgcgaggg cggatcgagc
     2101 cggattccgg cgattttc
//
LOCUS       DASUYC010001152         3552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_205822,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001152 DASUYC010000000
VERSION     DASUYC010001152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205822"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..951
                     /locus_tag="VF276_18805"
     CDS             34..951
                     /locus_tag="VF276_18805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006633763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEX6608958.1"
                     /translation="MAMSAPSPVRRAFADLVRKADADIDLARAALLIAQEEYPALDIE
                     GYLAQLDDLADLVRARLGLVPAAPARRRPIAPVAAPRAIITALNTVLFAERGFRGNRE
                     EYYDPRNSFLNEVLDRRTGIPITISLVYMEVAARLGLRLLGVGMPGHFIVQYPGVGNG
                     PGRYGRPQADELFIDPFNDGALLTRADCAAHFRRLYGPEFTFSGEFLTPMTRHQILAR
                     LLNNLKAIYLQRRDYNRAVVVVDRLLLVHPDAVWELKERGLLYYRLGAFLPALSDLRS
                     FLRHDPDAEDVPLVRYYADLCHRLLMIAN"
     gene            987..1736
                     /locus_tag="VF276_18810"
     CDS             987..1736
                     /locus_tag="VF276_18810"
                     /inference="COORDINATES: protein motif:HMM:NF013624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEX6608959.1"
                     /translation="MADTPAASVPRLLLTGFEGFGQFAVNPSWELAQRFQDQNIEMVH
                     VITAQLPVNWATAWSTLQAAIEQVDPRWVLMLGVGGTRRVVNAEVRGRNFTNPTRDNA
                     GELPPPNDLIEEGGPEYRIATIPATLLAERFQESGVPAEISTDAGGFLCNWVLYKALG
                     YAESRPGLQGVGFVHVPPLPEQGGPGVTLDQLERGLRLAIDTLVAGAAPLFNLVEAEI
                     EIERRQEIADRHLGQGLGVRNPTQPEGSAGA"
     gene            1774..2604
                     /locus_tag="VF276_18815"
     CDS             1774..2604
                     /locus_tag="VF276_18815"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEX6608960.1"
                     /translation="MSIAGGCQNALTRGRDVGCDAVQLFTKNASQWKAKPLLPEDVAR
                     FQATLEETGYSPDALVVHDSYLINLATPDDALWEKSIAAFGEELDRAGALGIPGVVTH
                     AGAHVGSGEEAGVTRIAAALDRVLSERSTGALVLLETTAGQGTCLGYRFEQIASMIEQ
                     SHYPDRIGVCLDTCHVFAAGYDLTTPEGYEGTLLEFDRLIGLDRLKAIHLNDAKKPLG
                     SRVDRHDHIGQGSIGLDAFRLLVNDPRLRHIPMVLETEKGPDGAEDRMNLATLRGLIS
                     "
     gene            2694..3329
                     /locus_tag="VF276_18820"
     CDS             2694..3329
                     /locus_tag="VF276_18820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020467428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEX6608961.1"
                     /translation="MPLARDPRPAEPPSILLVGQAPSRNEHESFAPPDDPLPPILLVG
                     QAPGLRATIEGVPFAGISGAKLRQWFALAGFSEADFWHLIYFSAVTKCYPGRLPGAKG
                     DRVPTPAEQALCRPWMDAQLAVLDPPIVVLVGQLAISTFLGKGPLHTFVGESFERDGR
                     RWLPLPHPSGVSTWLNDRANQARVAHAMAVLTRWMAELGIPVSGTPTPVPG"
BASE COUNT          541 a         1247 c         1191 g          573 t
ORIGIN      
        1 tggttacggc atctggtaga ggagcagcct cctatggcga tgagcgcccc ctcacctgtg
       61 cgccgtgcct tcgctgatct tgtccggaag gccgacgcgg atatcgatct ggcccgtgcc
      121 gccctgctga tcgcgcagga agaatacccc gcgctcgata ttgagggcta cctggcccaa
      181 ttggacgacc tggccgacct ggtgcgggcg cggttgggac tggtgcccgc cgccccggca
      241 cgtcgccggc ccatcgcgcc ggtcgcagcc ccgcgcgcca tcatcaccgc gctgaacacc
      301 gtgctcttcg cggaacgggg gttccgcggc aaccgcgagg agtattacga cccgcgcaac
      361 tcgttcctga acgaggtgct ggatcgtcgc accggcatcc cgatcacgat ctcgctggtc
      421 tacatggaag tggcggcccg gctggggttg cgcctgctgg gcgtgggcat gccggggcac
      481 tttattgtcc agtatccggg cgtgggcaac ggccctggtc gctatggccg gccccaggcc
      541 gatgaactct tcatcgaccc cttcaacgac ggcgcactgc tcacacgggc cgactgcgcg
      601 gcccatttcc gccggctcta tggtcccgag ttcaccttct ccggcgagtt tctcaccccc
      661 atgacccgcc accagatcct ggcccgcctg ctcaacaacc tgaaggcgat ctatttgcag
      721 cgccgcgact ataaccgcgc cgtggtggtg gtggaccggc tactgctggt gcatcccgac
      781 gcggtctggg aattgaaaga gcgcgggttg ctctattacc ggctgggcgc cttcttgccc
      841 gcgctgtccg acctgcgcag tttcctgcgg cacgaccccg acgccgagga tgtcccgctc
      901 gtccgctact atgccgatct ctgtcaccgg ctgctgatga tcgcgaacta gggcgcccgg
      961 cgccgccatg tagtgaggtt ttccctatgg cagatactcc cgcggcgagc gtgccgcgcc
     1021 tgttactgac cggttttgaa ggcttcggcc agttcgccgt caatccttcg tgggagctgg
     1081 cgcagcggtt ccaggaccag aacatcgaga tggtgcacgt gatcacggcc cagttgccgg
     1141 tcaactgggc cacggcctgg tccaccttgc aagccgccat cgagcaggtc gatccgcgct
     1201 gggtgctgat gctgggcgtg ggcggcacac gccgggtggt caacgccgag gtgcggggcc
     1261 gtaacttcac caaccccacg cgcgacaacg cgggcgaatt gccgccgccg aacgatctga
     1321 tcgaggaagg cgggccggag taccgcatcg ccaccatccc ggcgacgctg ctggccgagc
     1381 gcttccagga gagcggcgtg cccgccgaga tctcgaccga cgccgggggc ttcctctgca
     1441 actgggtgct ctacaaggcg ctgggctatg ccgaaagccg gcctggtctc cagggcgtgg
     1501 gcttcgtgca tgtgccgccg ctgccggagc agggcgggcc gggcgtgacg ctcgaccagt
     1561 tggagcgcgg gttacgcctg gcgattgaca cgctggtggc gggcgccgcg ccgctgttca
     1621 acctggtgga ggccgaaatc gagatcgagc gccgccagga gatcgccgac cggcacctgg
     1681 ggcagggact gggcgtgcgc aacccgaccc agccggaggg ttccgccggt gcctgagcaa
     1741 gccacgtcta ccgggccgcg cctgggcgcg catatgtcga ttgccggcgg ctgccagaac
     1801 gccctcacgc gcgggcgcga cgtgggctgc gatgccgtgc aactgttcac caagaacgcc
     1861 agccagtgga aagccaagcc gctcctgccg gaggatgtgg cccgcttcca ggcgacgctc
     1921 gaagaaaccg gctatagccc cgacgcgctg gtggtgcacg acagctacct gatcaacctg
     1981 gcgactcccg acgacgcgct ctgggagaag tccatcgccg cctttggcga ggaactggat
     2041 cgggccgggg cgctgggcat ccccggcgtg gtgacccatg ctggggcgca cgtgggcagc
     2101 ggcgaggagg cgggtgtgac ccgcatcgcc gccgcgctgg accgcgtgct gagcgagcgg
     2161 tccaccggcg cgctggtgct gctggagacc acggcgggcc agggcacctg cctgggctac
     2221 cgcttcgagc agatcgcctc catgatcgag cagagccatt accccgaccg catcggcgtc
     2281 tgcctggata cgtgccatgt gtttgcggcg ggctacgatc tgaccacgcc cgaaggctac
     2341 gagggtacgt tgctggaatt tgaccggctg atcggcctgg atcggttgaa ggcgatccac
     2401 ctcaacgacg ccaagaagcc gctgggcagc cgggtggacc gccatgacca tatcggccag
     2461 ggcagcatcg ggctggacgc gttccgcctg ctggtgaacg acccccgcct gcgccatatc
     2521 cccatggtgc tggagaccga gaaaggcccc gacggcgcgg aagaccgcat gaacctggcg
     2581 accctgcggg ggctgatctc ctgacacgcc gcgagcgact cctggccctg aatgccgaga
     2641 tccgggcctg ccggcgctgt cacgccgcgg gctacctgaa cgagcacgag tcgttgccgc
     2701 tggcccgcga tccccggccc gctgagccgc cgtcgatcct gctggtgggg caggcgccca
     2761 gccgcaacga gcacgagtcg ttcgccccgc ccgacgaccc cctgccgccg atcctgctgg
     2821 tggggcaggc gccgggcctg cgcgccacca tcgagggcgt gcccttcgcc gggatcagcg
     2881 gggccaagct gcgccaatgg ttcgccctgg ccggcttctc cgaggccgac ttctggcacc
     2941 tcatctactt cagcgccgtc accaagtgct accccggtcg cctgccgggc gccaagggcg
     3001 accgcgtgcc cacgcccgcc gagcaggccc tgtgccgccc ctggatggac gcgcaactgg
     3061 ccgtgctcga cccgcccatc gtggtgctgg tgggccagtt ggcgatcagc accttcctgg
     3121 gcaaaggccc gctgcacacc ttcgtcggcg agagcttcga gcgggatggc cgccgctggc
     3181 tgccgctgcc gcacccctcc ggcgtgagca cctggctcaa cgaccgcgcc aaccaggccc
     3241 gcgtggccca cgccatggcc gtcctcaccc gctggatggc ggaattaggc atccccgtca
     3301 gcggcactcc cacaccggta ccgggataat ccccgcccca gcgggcgccc ccatcccccg
     3361 tgtcaccctg agcgcagcga agggtctcgt ctaggcgcgg gccggtttca aaccggcccc
     3421 tacagcgcaa acgggcggtg gcgcaggcat gggtgcatgc cggaccccgg cggcaccatt
     3481 cggtgtgtcg ttcgtcatag tatacgaaga ggcacgcggc ctggactctt gccgcagggt
     3541 tattctgaca at
//
LOCUS       DASUYC010001153         2320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1823050, whole genome shotgun sequence.
ACCESSION   DASUYC010001153 DASUYC010000000
VERSION     DASUYC010001153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1823050"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1218)
                     /locus_tag="VF276_18825"
     CDS             complement(<1..1218)
                     /locus_tag="VF276_18825"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEX6608962.1"
                     /translation="MAKGWGAAGVALVLALTMWYYVGGVLIPYQQAEAAGSDRPRGNL
                     SDLYPRWLGSRELLLHGRDPYSAAVTREIQVGYYGRPIDPTRPHDPKDQQGFAYPVYV
                     AFLLAPTLGLDFGVVQSGFTWLLLALTAAGVPLWMYAVGWRPGMPIMLAAVLLTLGSF
                     PVVQGIALQQLSLLVGVLLAGAAAALVAGRPAPAGVLLALATIKPQVAAPLAAWLALW
                     TISDWRRRQGLAWGFGGTMALLLVGAEIASPGWIGRFRDALAAYQQYTDGRSLLATLL
                     TPIGGTVATVLLLALTALVCWRVRRDPAGTPGFALALALVPAVTLAIIPTWAPYNQVL
                     LLPGLFLLAAARDRIWARGRAARYCYLLAWGLVAWPWVATAALAAAALVLPAATVQSA
                     WTLPLYTSLLIPLG"
     gene            complement(1211..1954)
                     /gene="purQ"
                     /locus_tag="VF276_18830"
     CDS             complement(1211..1954)
                     /gene="purQ"
                     /locus_tag="VF276_18830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEX6608963.1"
                     /translation="MKFGVLVFPGSNGDHDCLYVLNEVLGVPTEAVWHEETRLAGYDC
                     LILPGGFSYGDYLRCGAIARFSPVMAPLRDYAAAGGPVLGICNGFQILTEAGLLPGAL
                     LRNQELAFSCRTIHVRVEPGARSTPFTAGVPGGAVWRLPIAHGEGRYFVEAAALADLE
                     AHQQIVLRYCTPDGVVTPDANPNGSTAGIAGVCNVAGNVLGLMPHPERAGEDLVGGSD
                     GLHLFHALIAACTRAGANLRAVPAGAAHG"
     gene            complement(2021..>2320)
                     /locus_tag="VF276_18835"
     CDS             complement(2021..>2320)
                     /locus_tag="VF276_18835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608964.1"
                     /translation="AHAATLPAPLHLFHEYGWGGYLIAHHVPVFVDGRADPYGNLLDD
                     YVAASSGARWEPVFATYGVNGVLIRTGAPLDKIIGATPGWSQVYHDSLSVLYIKR"
BASE COUNT          379 a          826 c          837 g          278 t
ORIGIN      
        1 cccgaggggg atcagcaggc tggtgtagag gggcagggtc cacgcgctct gcacggtggc
       61 ggcgggcagg accagcgccg ccgcggccag cgccgccgtg gcgacccacg gccaggccac
      121 caggccccag gccagcagat agcagtagcg ggcggcccgg ccccgcgccc agatgcggtc
      181 gcgggcggcg gccagcagga acaggcccgg cagcagcagc acctggttat agggcgccca
      241 ggtggggatg atcgccagcg tgaccgccgg gacgagggcc agagccagcg caaagccggg
      301 cgtgcccgcc gggtcgcgtc gcacccgcca gcagaccagc gccgtgagcg ccagcagcag
      361 caccgtggcg accgtgccgc cgatgggcgt gagcagggtc gccagcagcg agcgcccgtc
      421 ggtgtactgc tgataggcgg ccagcgcgtc gcggaagcgc ccgatccagc cgggcgaggc
      481 gatctccgca cccaccagca gcagcgccat ggtcccgccg aagccccagg ccagcccctg
      541 gcgccgccgc cagtcgctga tcgtccagag cgccagccag gcggccagcg gcgcggccac
      601 ctgcggcttg atcgtcgcca gggccagcag caccccggcc ggcgccgggc gccccgcgac
      661 cagcgccgcc gccgcgcccg ccagcagcac gccgaccagc aggctcagtt gctgcaaagc
      721 gatgccctgc accaccggga agctgcccag cgtgagcagc acggcggcca gcatgatcgg
      781 catgccgggg cgccaaccca cggcgtacat ccagagcggc acgccggcag ccgtcagcgc
      841 cagcagcagc caggtgaagc cgctctgcac caccccgaag tcgaggccca gcgtgggcgc
      901 cagcaggaag gccacgtaga ccgggtaggc gaagccctgc tgatccttgg ggtcgtgcgg
      961 gcgcgtgggg tcgatggggc ggccatagta gcccacctgg atctcgcggg tgacggcggc
     1021 gctgtagggg tcgcggccat gcagcagcag ttcgcgcgaa ccgagccagc gcgggtagag
     1081 atcggacagg ttgccgcgcg ggcggtcgct gcccgccgct tcggcctgct gataggggat
     1141 cagcacgccg cccacgtagt accacatggt cagcgccagc acgagcgcca cccccgccgc
     1201 gccccagccc ttagccatgc gcggctcccg ccgggacggc gcgcagattc gcgcccgcgc
     1261 gtgtgcaggc ggcgatcaga gcgtggaaca ggtgcaaccc gtcgctaccg ccaacgaggt
     1321 cttcgcccgc ccgctccggg tgcggcatca gccccagcac gttgcccgct acgttgcaca
     1381 ctcccgcgat gcctgccgtc gagccgttcg ggttggcgtc cggcgtcact accccgtcag
     1441 gcgtgcagta gcgcagcacg atctgctggt gggcttcgag atcggccagc gccgccgcct
     1501 cgacaaagta gcggccctcg ccgtgggcga tgggcagccg ccagacggcg ccgcccggca
     1561 cgcccgccgt gaacggcgtg gatcgcgcgc ccggctcgac gcgcacatgg atcgtgcggc
     1621 aggagaaagc cagttcctgg ttacgcaaga gcgcgcccgg cagcagcccg gcctcggtga
     1681 ggatctggaa gccgttgcag atgcccagca ccggcccgcc cgctgccgcg tagtcgcgca
     1741 gcggcgccat gaccggcgag aagcgcgcga tggccccaca gcgcaggtaa tcgccgtagc
     1801 tgaagccgcc gggcaggatc aggcaatcgt agccggccag ccgcgtctcc tcgtgccaga
     1861 cggcctccgt gggcacgccc agcacctcgt tcagcacata gaggcaatca tggtcgccgt
     1921 tggatccggg aaacaccagg acaccgaatt tcagttgtcg cacgtgcgcg ccttctttcg
     1981 tgtgagccgt cagccgtcag gaaccatgga gtgttggctg ctatctctta atatacagta
     2041 ccgacaggct atcgtgatag acctgcgacc agcccggcgt ggcgccgata attttgtcca
     2101 ggggcgcgcc ggtgcggatg aggacgccgt tcacaccgta ggtggcgaag acgggttccc
     2161 agcgagcgcc gctggatgcg gcgacgtagt cgtcgagcag gttgccgtag ggatcggcgc
     2221 gcccgtcgac gaagacgggc acatggtggg cgatcaggta gccgccccag ccgtattcgt
     2281 ggaacaggtg cagcggcgcg ggcagggtgg cggcgtgggc
//
LOCUS       DASUYC010001154         3403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1897830, whole genome shotgun sequence.
ACCESSION   DASUYC010001154 DASUYC010000000
VERSION     DASUYC010001154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1897830"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VF276_18840"
     CDS             complement(<1..221)
                     /locus_tag="VF276_18840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608965.1"
                     /translation="MARPTDTGDAPEATRVSRGTPGAISASPNTIGRLAFRWEGLLVV
                     LILAVTLLNSRLSPFFLNGTNLLRTTSDF"
     gene            complement(278..1294)
                     /locus_tag="VF276_18845"
     CDS             complement(278..1294)
                     /locus_tag="VF276_18845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6608966.1"
                     /translation="MAATTDAYARPERGWLQAVVRFREVGIILFILVLAVLVSLRTPA
                     FLTPTNFNNILLNIAILVIVALAQAMVIITRGIDLSVGSTIGLTAMMVAFTVVAFPGL
                     SAPLVVALGMALGAVLGGFNGLIVARGGVPPIIATLGTLSVYRGLIFLYSGGTWITAD
                     KMPPGFREIAKGTPLGVPNLILFAVVVIAAVYAFLKYTRTGRDIYTVGSNPTAARFAG
                     IPQERVIFLVFTISGLLCGLAGVLWASRFEAAQTNTAMGFELQTVAAPVVGGVNIFGG
                     SGSVLGVVLGAFLLGIINNALTLVGISPFWQLAVQGLLILLAVITDSAISRRLQRLTI
                     RRTR"
     gene            complement(1325..2815)
                     /locus_tag="VF276_18850"
     CDS             complement(1325..2815)
                     /locus_tag="VF276_18850"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018560953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX6608967.1"
                     /translation="MSDQTAVFKAEHISKSFGGVQALQDVHFEIFPGEVHALLGENGA
                     GKSTLIKIITGVHQPDSGTLFLDGQPIRFAGTREAQAHGIAGIYQEPSLFPDLDIAEN
                     ILVGRQPVRRGAVDWKRMYQEAGVLLRRLGLALDPRTKARDLSVAQQQMVEIARALSI
                     NAKILIMDEPTSSLTLHEVEELFAIVRQLRAAGTAVVFISHRLEELFALADRVTILRD
                     GTYVGTRSMTEVTPDDLIRMMVGRTLGELFPKQAIAPGEVILEVENLGVEGSFSGVSF
                     QLRRGEILGMAGLIGAGRTNVARALFGTEPATAGTIKLDGKIVTIANPDTAMALGIGY
                     VPEDRKEHGLVLEMSIGRNITLPILPRFTRLGWMDERRERQAASADARRLEVKMAGVG
                     QKAGELSGGNQQKVVLAKWLGTQPRVLILDEPTRGIDVGTKAAVHGLMSNLAAQGMAI
                     LMISSELPEVLGMSDRILVMREGRLTGQFSRAEATQETIMAAATTA"
     gene            complement(3155..>3403)
                     /locus_tag="VF276_18855"
     CDS             complement(3155..>3403)
                     /locus_tag="VF276_18855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEX6608968.1"
                     /translation="VGKPSPIMVATVLAMLDLPAARCIMIGDRLETDIRMGLSAGMAT
                     CLVLTGDATPAKLAASGMQPTLVLDRIDGLLDADRAAP"
BASE COUNT          588 a         1090 c         1157 g          568 t
ORIGIN      
        1 atgaaatcgg aggtggtgcg cagcaaattg gtgccgttca agaagaaggg cgacagccgg
       61 ctgttcagca gggtcacggc caggatcagc acgaccagga ggccctccca gcggaaggcc
      121 aggcgcccga tcgtgttggg cgatgcggag atcgcgccgg gcgtgccccg gctgacccgc
      181 gtcgcctccg gcgcgtcgcc tgtatcagtt ggccgagcca tgtccactct ccttgggcct
      241 ggtgggcggc tgtcgcccaa atggtgcgcg ctgcatatca gcgggtgcgc cggatcgtga
      301 gccgttgcag gcgccgcgag atggccgagt cggtgatgac ggccagcagg atgagcagcc
      361 cctgcacggc tagctgccag aagggcgaga tgccgaccag cgtgagcgcg ttgttgatga
      421 tgccgagcag gaaggcgccc agcaccacgc ccagcacgct gccgctgccg ccgaagatat
      481 tgaccccgcc gacgaccggc gccgccacgg tctgcaactc gaagcccatg gccgtgttcg
      541 tctgcgccgc ctcaaagcgc gacgcccaca acacgcccgc cagcccgcaa agcaggcccg
      601 aaatggtgaa cacgaggaag atgacccgct cttgcgggat gccggcaaag cgggccgccg
      661 tgggattgct gcccaccgtg tagatgtccc gccccgtgcg ggtgtatttc aggaaggcgt
      721 agacggcggc gatcaccaca acggcgaaca ggatcaggtt gggcaccccc aggggcgtgc
      781 ccttggcgat ctcgcgaaag cccggcggca ttttgtccgc ggtgatccag gtgccgccac
      841 tatagagaaa gatcagcccg cgatagacgc tgagcgtgcc cagcgtggca atgatcggcg
      901 gcaccccgcc gcgggccacg atcaatccgt tgaagccgcc gagcacagcg cccagcgcca
      961 tgcccagcgc gacgacgagg ggcgcggaca ggccggggaa ggccacgacc gtgaacgcga
     1021 ccatcatcgc cgtcagcccg atagtcgagc cgacggacag gtcgatgccg cgggtgatga
     1081 tgaccatggc ctgcgccagg gcgacgatca ccaggatggc gatgttcagc aggatgttgt
     1141 tgaaattggt gggcgtgagg aaggcgggcg tgcgcaggct caccagcacg gccagcacca
     1201 ggatgaacag gatgatgccc acctcgcgga agcgcacgac cgcctggagc cagccgcgct
     1261 ccgggcgagc gtaggcgtca gtcgttgcag ccatccccga tgctcccttc cccgttgtgc
     1321 gcggctaggc cgtcgtggcg gcggccataa tcgtttcctg agtcgcttcg gcacggctga
     1381 actggccggt cagccgccct tcgcgcatca ccaggatgcg gtcgctcatg cccaggacct
     1441 ccggcaattc ggaggagatc atcaggatcg ccatgccctg cgcggcgagg ttgctcatca
     1501 agccatgcac ggcggctttg gtgcccacgt cgatcccgcg cgtcggctca tcgaggatga
     1561 gcacgcgggg ctgggtgccc agccacttcg ccagcactac cttctgctga ttgccgccgg
     1621 acagttcgcc ggctttctgg cccacgccgg ccatcttcac ttccagccgc cgggcatcgg
     1681 cgctggcggc ctggcgctcg cggcgctcgt ccatccagcc caggcgggtg aaccggggta
     1741 ggatcggcag cgtgatgttg cggccaatac tcatctccag caccagcccg tgttccttgc
     1801 ggtcttcggg cacatagccg atgccgagcg ccatggccgt gtcgggattg gcgatggtga
     1861 cgatcttgcc gtccagcttg atcgtgcccg ccgtggcggg ttccgtgccg aacagggcgc
     1921 gggccacgtt cgtgcgcccc gcgccgatca gcccggccat gcccaggatc tcgccccggc
     1981 gcaactggaa agacacgccg gagaaactgc cttcgacgcc caggttttcg acctccagga
     2041 tcacctcgcc gggcgcgatc gcctgcttgg ggaataattc gcccagcgtg cggcccacca
     2101 tcatgcggat caggtcgtcg ggagtcacct cggtcatcga gcgggtgccg acataggtgc
     2161 cgtcgcgcag gatcgtgacc cggtcggcca gggcgaagag ttcttccagg cggtgcgaga
     2221 tgaacacgac ggcggtgccg gcggcgcgca actggcgcac gatggcgaat agctcctcga
     2281 cttcatgtag cgtgagcgag gaggtcggtt cgtccatgat caggatcttg gcgttgatcg
     2341 acagggcgcg ggcgatctcc accatctgct gctgggccac gctcaggtcg cgggctttgg
     2401 tgcgcgggtc gagggcgagg cccagccgcc gcagcagcac cccggcttcc tggtacatgc
     2461 gcttccagtc cacggcgccc cggcgcaccg gctggcgccc caccaggatg ttttcggcaa
     2521 tatccaggtc ggggaacagg ctcggctcct ggtagatccc ggcgatgccg tgtgcctggg
     2581 cctcgcgagt gccggcaaag cggatcggct gcccgtcgag gaagagggtg ccgctatcgg
     2641 gttggtgcac gccggtgatg atcttgatca gggtggactt cccggcgccg ttctcgccca
     2701 gcagcgcgtg gacttcgccg gggaagatct cgaagtggac atcctgcagc gcctgcacac
     2761 cgccaaagga cttggagata tgttccgctt tgaacaccgc agtctggtca ctcatcgcgc
     2821 atactccgtc gcctgcggcg ccccgatctg cccaggcacg cgcatgaaaa aagcaagcgc
     2881 gcttcgggaa ttgtcgatct tcagcctgaa gtgggggtca agccgaagcg agcgaaaggg
     2941 tagcgaactg gtaaggctcc gattgggtta tcatagcatg gctgttcgca aaagtcaata
     3001 ggttagtgaa catgtatttt cgtatcgttt acggaacgat ctgctactac ccatgctcta
     3061 tgctatgcgg cacgtatatt gcggcttcga cttatgaagc tgttccgaat acggtggggc
     3121 ttgctggata gattgcgtcc gcccggcgcg cgagtcacgg cgcagcccga tcggcatcca
     3181 gtaagccgtc gatgcggtcg aggaccaggg tcggctgcat cccgctggcg gcgagcttgg
     3241 cgggggtcgc gtcgccggtg agcacgaggc aggtcgccat gccggcgctg aggcccatgc
     3301 ggatgtcggt ttccagccgg tcgccgatca tgatgcagcg ggccgccggc aagtcgagca
     3361 tcgccaggac cgtggcgacc atgatcggcg agggcttgcc gac
//
LOCUS       DASUYC010001155         4823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_337444,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001155 DASUYC010000000
VERSION     DASUYC010001155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337444"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VF276_18860"
     CDS             complement(<1..185)
                     /locus_tag="VF276_18860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608969.1"
                     /translation="MKPLRLRVRVALVLGVLMCAALAAGWVRAAPGAAPAAGPATGMR
                     VGYARKHDLSPPLGSLPP"
     gene            810..1175
                     /locus_tag="VF276_18865"
     CDS             810..1175
                     /locus_tag="VF276_18865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012322382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4260 domain-containing protein"
                     /protein_id="HEX6608970.1"
                     /translation="MLVQSLTRPTLLLRVEGGALLLAALVLYGQTGGNWLLFVLLLLA
                     PDLSMLGYRAGNATGAAVYNLAHTEVFPALLGAAGLLAGQPLLLALALIWFAHIGMDR
                     VLGYGLKYPSGFKQTHLGA"
     gene            complement(1195..3030)
                     /locus_tag="VF276_18870"
     CDS             complement(1195..3030)
                     /locus_tag="VF276_18870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608971.1"
                     /translation="MPEPPATGPFASIEDLDPAPARPASQMPRWEVVLGVLLVLAVTG
                     FASLQWWDQQTRLTHYREATRAMAALDWTAAHRAFLAAAGYADADKRAADAAGLEKAR
                     NTAYAAAGAADAAGHWTDLVAPAARLRTLGPQYRDTPRLIAAVEQHVYQPTLSGTVVL
                     RPDASPPGLYTYEAGGWRALPGSDRTSTVLATCPDGLLVYDAAWRGSGPVPTPAPGRL
                     PVRRVLVGARGGTAPVALALDPDRFDLFRCTDAGVWGIRTLTTLPGAPASAPVPQRLV
                     AYQAFGTTTPISPVDLGTNWMIAGPAPDGRHLLLYETSALTGAGGQTGLYVADADGRN
                     PRRLAGLPGAPGPDLPVAAGPDLVVSAYLPVAGTRAYTNTVLLVDPAGARPPHPVVEV
                     RVPAATFDDHFQVQIQVVATGPRAGQVLVIWPADSGTVVRLVDPVHPARPWYDIYLSE
                     RQNARVLATDSADGGLILAASRVTAQSRRPDDRLVYIDPHDRATEIAESAGSLDQILT
                     AWMRGDYLVYATGAGPNIGFGGPKRYIFRSLPRGQLTGIPTVVYAATVDRLWPSLGPP
                     WWAGADLLAYLTPDGELHARSYDGHQDVVLDTGVTAFARETFVTP"
     gene            complement(3251..4702)
                     /locus_tag="VF276_18875"
     CDS             complement(3251..4702)
                     /locus_tag="VF276_18875"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEX6608972.1"
                     /translation="MSRAAGAGLTGPPRAVAGIDREAELIGLAVTLLDVLALHPLLAL
                     IAGPGSDGTARAALWPLVALAVLARGVVWLLEQRRASATVYTAVTALAGLGSLAALVW
                     VEFYAAPGAGWDALVRDLQHFSVTGLKLIALVLLGGYIWWRSLRGDAPALGAIYTRFE
                     RIVVAYVAYALITWVAAARFMQPVFTRDLFLVFLTALWAFALARSRMEGARSRQHLGT
                     RWFVFSLGLIAAILAAGMLVAGLFARDVADALFGPLWTLVQWIGVVVVGILAALTLII
                     SSLLEFFFGFLRGLPRAPATPTPTPGPTGGNLIDRLREQAPGGPPADTTLFAVIGIIV
                     LLVLALWAARAVLGSAQRRRHSLGGGERESLFNWGALLGSLQRARPAPGPEADSLRDL
                     AGNPAYRYTVRVRRAYRRALARAAAHEVTRTPAQTADEVLPALQGAFPAARDPLRDLT
                     ALYDATRYTATPATAADAEAAEAAARALDTPDR"
BASE COUNT          788 a         1666 c         1769 g          600 t
ORIGIN      
        1 ggcggcaggc tgcccagcgg cggcgacaga tcatgcttcc gcgcgtagcc cacgcgcatc
       61 ccggtcgccg gacccgccgc cggcgccgcg ccgggggccg cccgcaccca gcctgccgcc
      121 agcgcggcac acatcaagac ccccagaacg agcgcaacgc gcacgcgcaa acgcagaggt
      181 ttcatagcta ttcactttct cgccggactc agcggggaat ctgcgcgaac ctgcgcgccg
      241 cccttggcgt gaccgaggcg acgcaccaaa atcgccccgg ttacacaggg ctaagtagct
      301 gtcacaccaa tcgcgccatt ccgagcgcag cgaagaatct ccgtcgcgta gcccaaacga
      361 cggacgcttc gcccagaatg acaaacacgc ggtgacaaac taccaggtac cgcaaacagc
      421 gcgcgatctg tgtccaggcc ggcaaggggg cggcagtagt ggatggggtg agactacgcg
      481 ggcacaaaga gtaagcgcac gatgctaccg ggaagcggac ggatcgggtc catgcagaac
      541 gccaacgggg aggtcgcaca ttggccgcgg ggcactccct acacccttat tctaaccgat
      601 caacccgctc gccacaatcc atccgcggcg caacgaacac cggcgccgtc catataccgg
      661 ccacccggct gacacaacca gggctcggcc aaaagacgga ggcaaagtcg ggacaaacat
      721 cgcttggcaa ttatagaatg gtattctata attaaaatgt agaacggaat tctagattat
      781 gagactcagg atgatggagg tccaattcga tgctggtgca atcgctgacg cgcccgaccc
      841 tgctgctgcg ggtggaaggc ggcgcgctgc tgctggcggc gctggtgctc tatgggcaga
      901 cgggcgggaa ctggttgctg ttcgtgctgc tgctgttggc gcccgacctg tcgatgctgg
      961 gctatcgggc gggcaacgcc accggcgccg ccgtctataa cctggcccac accgaggtct
     1021 tcccggccct gctgggcgcc gcgggcctgc tggccgggca gccgttgctc ctggccctgg
     1081 cgctgatctg gttcgcccat atcggcatgg accgggtgct gggctacggg ttgaagtatc
     1141 ccagcggctt caagcagacc cacctgggcg cgtaggcccc accgccgggg gcgctcaggg
     1201 tgtgacgaag gtttctcgcg cgaacgccgt cacgccggta tcgagtacta catcctggtg
     1261 accgtcatag ctgcgggcgt gcagttcgcc atcgggcgtc agataggcca gcagatcggc
     1321 gcccgcccac cagggcggcc ccagcgacgg ccagaggcga tccaccgtcg ccgcgtagac
     1381 gacagtggga atgccggtga gttgcccgcg cgggaggctg cggaagatat agcgcttggg
     1441 gccgccaaag ccgatgttcg ggccggcccc ggtggcgtag acaagatagt ccccgcgcat
     1501 ccaggcggtc agtatttggt ccagactgcc ggccgattcc gcgatctcgg tcgcccgatc
     1561 gtgcgggtca atatagacga ggcggtcgtc gggccggcgg ctttgtgccg tcacgcggga
     1621 ggccgccaga atcagcccgc cgtcggcgga atcggtagcc agcacgcgcg cgttttggcg
     1681 ctcgctgaga tagatgtcgt accagggccg ggccggatgc acgggatcga ccaggcggac
     1741 gaccgtaccg ctatcggcgg gccagatcac cagcacctgc ccggcgcgcg gcccggtcgc
     1801 cacgacctgt atctggacct ggaaatggtc gtcgaaagtg gccgcgggca cgcgcacctc
     1861 gacgaccggg tggggcggcc gcgcgcccgc cgggtccacc agcagcaccg tgttggtgta
     1921 ggcgcgggtg ccggccacgg gcaggtacgc gctcaccacc agatcgggtc cggcggccac
     1981 gggcaaatcg ggaccgggcg cgccgggcag gccggccagc cggcggggat tgcgcccatc
     2041 ggcatcggcc acgtacaggc cggtctgccc gccggcgccg gtcagggcgc tcgtctcgta
     2101 gagcagcagg tgccgcccgt cgggcgcggg gccggcgatc atccaattag tgcccagatc
     2161 taccgggcta atcggggtgg tggtgccgaa agcctggtag gccaccaggc gctgcggcac
     2221 cggtgcgctg gccggggcgc cgggcagcgt ggtcagcgtg cggatgcccc acaccccggc
     2281 atcggtgcaa cggaacaggt cgaagcggtc gggatcgagc gcgagggcga cgggcgcggt
     2341 cccgccacgg gcacccacca gcacccggcg taccggcagg cggcccggtg cgggagtcgg
     2401 caccggaccc gacccccgcc aggcggcatc atagaccagg aggccatccg ggcaggtcgc
     2461 cagcacggtg ctggtgcggt cgctgccggg cagggcgcgc cagccacccg cctcgtaggt
     2521 atagagcccc ggcgggctcg catcgggccg caggacgacc gtgccgctca gagtcggctg
     2581 gtagacatgc tgctcgacgg cggcgatcag gcggggcgtg tcgcggtact gtggtcccag
     2641 cgtgcgcagc cgggccgccg gcgccacaag atccgtccaa tgcccggcgg catctgccgc
     2701 accggcggcg gcgtaggccg tgttgcgcgc cttttccagg ccggcggcat cagccgcccg
     2761 cttgtccgcg tcggcgtagc cggcggcagc gaggaatgcc cggtgcgccg ccgtccagtc
     2821 gagcgcggcc atggcccgcg ttgcctcacg gtagtgcgtg agccgcgtct gctgatccca
     2881 ccactgcaac gaggcaaacc cggtcacggc cagcaccagc agcacgccca gcaccacctc
     2941 ccagcgcggc atctggctcg ccgggcgggc gggcgcgggg tccagatcct cgatggacgc
     3001 gaacgggccg gtggcgggcg gctcgggcac agacacaacc tccagggggc ggggccggcc
     3061 ctagtggtgc cccgctcatt ctcttgccga cctcactggc cgccgcctcg gccatccgcg
     3121 ggcggcgcac tcgatggggt tcccctctag aatagcacat ccggccgccg ggatgcgact
     3181 cgtagtttcc ggcaggggtc ggcccgatcg cccggcacag caggaggtaa gccccgccac
     3241 gcggggaggg ctagcgatcc ggcgtgtcca gcgcgcgggc ggcagcctcg gcggcttcgg
     3301 catcggcggc ggtggccggg gtggcggtgt agcgggtggc gtcgtagagc gcggtcagat
     3361 cgcgcaacgg gtcgcgggcg gcggggaagg cgccctgcaa cgcgggcaac acctcgtcgg
     3421 cggtctgggc cggggtgcgg gtgacttcgt gcgcggcagc gcgggccagc gcccggcggt
     3481 aggcccggcg cacgcgcacg gtgtagcggt aggcggggtt gccggcgaga tcgcgcagcg
     3541 agtcggcctc cgggccgggc gcggggcggg cgcgttgcaa cgaccccagc agcgcgcccc
     3601 agttgaacag cgactcgcgc tcgcccccgc ccaggctgtg gcgccgccgc tgcgcgctgc
     3661 ccagcacggc gcgggcggcc cacagggcca ggaccagcag cacgatgatg ccgatcaccg
     3721 cgaacagggt ggtatcggcc ggggggccgc cgggcgcctg ttcgcgcagc cggtcgatca
     3781 ggttgccgcc ggtggggccg ggcgtaggcg tcggcgtggc cggggcgcgc ggcaggccgc
     3841 gcaggaagcc gaagaagaac tccagcagcg aactgatgat gagagtcagc gccgcgagga
     3901 tgcccacgac gaccacgccg atccactgca ccagggtcca gagcgggccg aacagggcat
     3961 cggccacgtc gcgggcgaac aacccggcca ccagcatgcc tgcggccagg atcgccgcga
     4021 tgaggcccag cgagaacaca aaccagcgcg tgccgaggtg ctggcggctg cgcgctccct
     4081 ccatgcgcga gcgagccagc gcgaacgccc acaacgccgt caggaacacc aggaacaggt
     4141 cgcgggtgaa caccggctgc atgaaccggg cggcggccac ccaggtgatc agggcgtagg
     4201 ccacgtaggc gacgacgatg cgctcgaagc gggtgtagat ggcgccgagc gcgggcgcat
     4261 cgccgcgcaa tgagcgccac cagatgtagc cgcccagcaa cacgagcgcg atcagcttca
     4321 gcccggtcac ggaaaagtgc tggagatcgc gcaccagcgc atcccacccg gcgccgggcg
     4381 cggcgtaaaa ctcgacccag acgagggcgg ccaggctgcc cagcccggcc agcgcggtca
     4441 cggcagtgta gacagtagca ctcgcgcgcc gctgctccag cagccagacc acgccgcggg
     4501 ccaatacggc tagagcaacc agcggccaga gcgccgcccg cgccgtccca tcgctgcccg
     4561 gcccggcgat gagcgccagc agcgggtgca gcgccagcac gtcgagcagc gtcacggcca
     4621 gcccgatcag ctcggcctcg cggtcgatgc cggccacggc gcgcggcggg ccggtcagcc
     4681 cggccccggc ggcgcggctc atggggccac ctccgcgggc gtggcggccc cggcgggctg
     4741 tgagtcgggt gcgggcgcgt cgtcgtcaaa cagggtgcgc aggctgggca gcgggggcgg
     4801 gcgcaggtag ggcgcatcgg cgg
//
LOCUS       DASUYC010001156         2199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1594105, whole genome shotgun sequence.
ACCESSION   DASUYC010001156 DASUYC010000000
VERSION     DASUYC010001156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1594105"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VF276_18880"
     CDS             <1..197
                     /locus_tag="VF276_18880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608973.1"
                     /translation="PTGASLLKLPLDLDAWHRALPDDLLAQGVAIIDSRDALVGKQAE
                     RGQAVPAGSGGHARQQTARA"
     gene            491..1501
                     /locus_tag="VF276_18885"
     CDS             491..1501
                     /locus_tag="VF276_18885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX6608974.1"
                     /translation="MKYFVTGATGFIGGRVVRQLRAAGHEVVTVARNPAKAGDLAALG
                     VDVRPGDITERATLREPMTGVDGVFHIAAWYKIGARDKSPARPTNVEGTRNVLETMRD
                     LGIPKGVYTSTLAVFGDTHGVTADENYRAGGPWLSEYDHTKWQAHYEVAEPLMKAGLP
                     LVIVQPGLTYGPGDTSAVHDTLVRYLKGKLPMAPQRTTFCWGYVDDVAQGHILAMERG
                     RPGESYIIAGSPHTFIEALTLAQQISGVPAPRQHPPPALLKGLAAVMGVVGAVVPLPQ
                     DYTAESLRSIAGVTQLATNAKARRELGYNPRPLADGVAETLAAEMAALGMPAPKPAAS
                     AR"
     gene            complement(1606..>2199)
                     /locus_tag="VF276_18890"
     CDS             complement(1606..>2199)
                     /locus_tag="VF276_18890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608975.1"
                     /translation="LRRVVEFLNGQMDPAEVLAIEIRQYVGQNLKTLVPRVVGQTANA
                     EQKKTAGDADIRQWDEDGFFQELTARKGDEAAHVARQILDWAKAHGLRIWFGKGKKDG
                     SFFPLLDYKGTTYWLMAVWTYGRVDIQFQYMAGPFATDAGRLAVLQRLNAIPGLTLAA
                     NTITRRPSIPLQTLAEPSRIQQFLAVFDWMISEIKAT"
BASE COUNT          376 a          724 c          732 g          367 t
ORIGIN      
        1 cgcccaccgg ggccagtctg ctcaaattgc cgctcgatct cgacgcctgg caccgcgccc
       61 tccccgacga tttgctggcg cagggagtgg cgatcatcga ctcgcgggac gccctggtcg
      121 gcaagcaggc cgagcggggg caggccgtcc cggcgggatc gggagggcac gcccggcagc
      181 aaaccgcgcg ggcttaaagc gcgcctccat ccctaccttt ccggcgcgaa ccagatatgc
      241 cggcatgccg gcggcgggtg gcccacaggg tggccggact gcggtataat gcatgcaaga
      301 agcagtgccg gccctccacc ctatccgttc cccacttggc ccgaaaaccg tctcgctcag
      361 ctttaccgtg gttgccctgg tcgtaaggcg gtgcgccgca tcggcccggc gcgggcggat
      421 ctgcttcgcg tgtgctggcc cggtatgatc ggcgcggtct cataccggca ccgaaaagga
      481 gcgatgcgct gtgaaatact tcgttaccgg ggcaacgggc tttatcgggg ggcgggtggt
      541 gcggcaattg cgggcggccg ggcatgaggt cgtcaccgtg gcccgcaacc cggccaaagc
      601 gggcgacctg gccgcgctcg gcgtggatgt ccggcccggc gacatcaccg agcgggccac
      661 gctgcgtgag ccgatgacgg gcgtggacgg tgtgttccat atcgccgcct ggtacaagat
      721 cggggcgcgt gataagtcgc cggccaggcc gaccaacgtc gagggcacgc gcaacgtgct
      781 ggaaacgatg cgcgacctgg gcatccccaa gggggtctac accagcacgt tggccgtctt
      841 cggcgacacc cacggggtga ccgccgacga gaactatcgc gcgggcggcc cctggttgag
      901 cgagtacgac cacaccaagt ggcaggcgca ttatgaggtg gccgagccgc tgatgaaggc
      961 gggcctgccg ctggtgatcg tccagccggg gctgacctac gggccgggcg ataccagcgc
     1021 cgtgcatgac actctcgtgc ggtatctcaa gggcaagctg ccgatggctc cgcagcgcac
     1081 taccttctgc tggggctacg tcgatgacgt tgcgcagggg catatcctgg cgatggagcg
     1141 gggccggcct ggcgagagct atatcatcgc cggctcgccg cacactttca tcgaggcgct
     1201 gactctggcc caacagatca gcggcgttcc cgcgccccgg cagcacccac cgcccgccct
     1261 gctcaagggg ctggccgccg tgatgggcgt ggtgggcgcg gtcgtgcccc tgccccagga
     1321 ttataccgcc gagagcctgc gcagcatcgc cggcgtgacc caactcgcca cgaacgccaa
     1381 ggcgcggcgc gaactgggct acaacccgcg cccgctggcc gacggcgtgg ccgagacgct
     1441 ggcggcggag atggcggcgc tgggcatgcc cgccccgaag cctgcggcga gcgcgcggta
     1501 acgcgccggg gactatcgcc ggagcgcacc ggcaccggcg cgctccggat cgccgagcgc
     1561 gatctggtgc ttcgtgccgg gcagagtggc ccgatgcccc ggcgctcatg ttgctttaat
     1621 ttcggagatc atccaatcga acacggcaag gaactgttgt atacgcgacg gctctgccaa
     1681 ggtttgcagg ggaatgcttg ggcgacgggt gatcgtattg gccgcaagag tgaggcctgg
     1741 aatggcgttc aaccgctgga gcaccgccaa gcgcccggcg tcggtagcga aaggcccggc
     1801 catatactga aactgaatat ccacccgccc ataggtccag acggccatca gccagtatgt
     1861 ggtgccttta taatcaagca atggaaaaaa cgatccatct ttcttgccct tcccgaacca
     1921 gatccgcaag ccgtgggctt tagcccaatc gaggatctgt cgggcaacat gggcagcctc
     1981 gtcacctttt cgtgctgtaa gctcctggaa aaagccgtct tcatcccatt gccggatatc
     2041 cgcgtcgccg gcggttttct tctgctccgc gtttgccgtt tgcccaacga cccgaggaac
     2101 cagggtcttc aagttctgtc ctacatattg gcggatttca atcgccaaca cctctgccgg
     2161 gtccatctgt ccgttcagga actcgaccac gcggcgcag
//
LOCUS       DASUYC010001157         2871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1133076, whole genome shotgun sequence.
ACCESSION   DASUYC010001157 DASUYC010000000
VERSION     DASUYC010001157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133076"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1218)
                     /locus_tag="VF276_18895"
     CDS             complement(<1..1218)
                     /locus_tag="VF276_18895"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX6608976.1"
                     /translation="MDDHLLQHLTPAALARNELFQGLSTDELERLSQRLRFRRAKKGA
                     VLCKEGDPSDSMYIIEAGQIRVYKETPDGRILLATAGPGSHVGEMALLTGDARSATLV
                     VSIDAELWELTKGDFDEVLREYPGIPLTFSRVLAKRLSQADRRIVLEETSHLVALVGT
                     PQETVRLAESVYRQSGRTVLLLDLVETGDECSTIDPADPFHDVIGIAEGVSRVDVPVE
                     VSSGDFSEIASRLLLRFNHLLLRLPDYSGTYLYQALDLCDSVIIFGQQIDHWVFRSPV
                     PANRIWQVMGPDHRRYTPERGERDIDRLARRVAHMSVGLALSSGAAHGLAHIGVLRVL
                     IQNQIPIDMVAGTSMGSLIGAAFVSGADPDGLYKLGKEFSGFKKLSAWRIWDFQFPRS
                     GVVRGNAAKRWIDH"
     gene            1519..>2871
                     /locus_tag="VF276_18900"
     CDS             1519..>2871
                     /locus_tag="VF276_18900"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX6608977.1"
                     /translation="MVDPGAHAATVNPLAQPVWTQLNNTILAATGWRTVLADCAGAVV
                     DTGAAAPGAAESPPLSAGLVQAVQTTCRNGTPRVVLDDSRDGLPVRWAVVPIVIGGQA
                     LGVLLAGGLPAAEDVPGGPSIGQADADQACRLIGGLVTAIAGLYSAAPAQAAVQDRYL
                     AAQRRAAALYDLSTLITSTLELGPIVELLLTQTRQLFGADRAAVFLRGDGGELRCVNA
                     VGLSGEYLSAVNQMYEQAAGGRVEQTGRTLVVEDATASPTMGPLRVMAQREGFRSMVV
                     TPFVYHGKAIGALTLYHDNPHEYSDDDVSALTTFANHVAVALGNARLFEQAQRQVRRS
                     NFLAEAGRLLNSSLDMGHVLNSLSRSAVEVLGEACAIYLLRKNEDELALTAYSDQLDE
                     NIRDRVAFLEAHPPRLGATGIGLAALRGESLLVDAQHPRPDSGPDLYLEGLGAHSYLV
                     V"
BASE COUNT          449 a          966 c          967 g          489 t
ORIGIN      
        1 gtggtcgatc cagcgcttgg cggcattgcc gcgcaccacg ccggatcggg ggaactggaa
       61 gtcccagatg cgccaggcgc tcagtttctt gaacccggag aactccttgc ccagtttata
      121 cagcccgtcc ggatcggccc cgctgacaaa agccgccccg atcaaggacc ccatgctggt
      181 gcccgccacc atatcgatgg gaatctggtt ttgtatgagc acgcgcaaca cgccgatatg
      241 cgccagcccg tgcgccgccc cgctgctcag ggcgaggccg acgctcatat gggccacgcg
      301 ccgcgccagc cggtcaatat cgcgctcgcc ccgttcggga gtgtagcgcc ggtgatccgg
      361 ccccatcacc tgccagatcc ggttggcggg cacgggcgac cggaacaccc agtggtcgat
      421 ctgctggccg aaaataatca cgctgtcgca caggtcgagc gcctggtaca ggtatgtccc
      481 gctataatcc ggcaggcgca gcagcaagtg gttgaagcgc agcagcaggc gcgaggcgat
      541 ctcgctgaaa tcgccgctgc tgacctccac cggcacatcg acgcgcgaca cgccttcggc
      601 gatcccgatc acgtcgtgga aggggtctgc cgggtcgatg gtgctgcact cgtcgccggt
      661 ttcgaccagg tccagcagca gcaccgtgcg cccgctttgc cggtagacac tttccgccag
      721 gcgcaccgtt tcctgcggcg tgccgaccag cgccaccagg tgcgaggtct cttcgagcac
      781 aatgcggcgg tcggcctggc tcaggcgctt cgccagcacc cggctgaagg tcaggggaat
      841 accggggtac tcgcgcagca cctcgtcgaa gtcgcccttg gtcagttccc agagttcggc
      901 gtcgatgctg accaccagcg tggcgctgcg ggcatcgccg gtcagcaggg ccatctcgcc
      961 cacatggctg cccggcccgg cggtggcaag caggatacgg ccatccggcg tctctttgta
     1021 gacgcggatc tgccccgctt cgatgatata catcgaatcg ctggggtcgc cctccttgca
     1081 gaggacggcg cccttcttcg cccggcggaa gcgcaggcgc tgcgacagcc gttccaactc
     1141 atcggtcgaa aggccctgaa atagttcgtt gcgcgccagt gccgcggggg taagatgctg
     1201 cagcaggtgg tcgtccatgg cagcattgta tactggccct ctgtgcttgt caatgggcca
     1261 gcggtccccg ccggcccccg ccggcaggcc ctatgaggcg gggagtggcg ttccccttcc
     1321 cgcgccggga agggggccag ggggttaggt tggtcccctg cggcgatgac cgccctcttg
     1381 accgtataga tccgaatcag tatactggtg atcaagtaca aaacgtggta cccgaattgt
     1441 ccgattttgt ccttacgggc atgtatgaac gggggcgctc tttgcacctg actcggatgg
     1501 ccgtctggtg gtatcggcat ggttgatcct ggtgcccacg cggcgacggt aaacccgctg
     1561 gcccagccgg tctggaccca gcttaacaac acgattctgg cggccaccgg gtggcggacc
     1621 gtgctggccg actgtgccgg ggccgtcgtg gacaccgggg ccgcggcgcc aggggcggcg
     1681 gaatcgccac cgctgagcgc ggggctggtg caggccgtcc agacgacgtg ccggaacggt
     1741 actccgcgcg tggtgctgga cgatagccgg gacggcctgc cggtgcgctg ggccgtggtg
     1801 ccgatcgtca tcggggggca ggcgctcggt gtgctgctcg ccggggggtt gccggccgcc
     1861 gaggatgtgc caggcggccc cagcatcggc caggcggatg ccgaccaggc gtgccggtta
     1921 atcggcggcc tcgtcacggc catcgccggg ttgtacagcg ccgccccggc gcaggccgcc
     1981 gtgcaggatc gctacctggc cgcgcagcgg cgcgcggcgg ctctatatga cctctccacc
     2041 ttgattacct cgaccctgga actcggcccc attgtcgaat tgctgctcac tcagacccgc
     2101 cagctatttg gcgccgaccg ggcggcggtg ttcctgcgcg gcgacggcgg ggagttgcgc
     2161 tgtgtgaatg ccgtcggcct ctccggcgaa tacctgagcg ccgtcaacca gatgtatgag
     2221 caggccgcgg gcgggcgcgt cgagcagaca ggccgtaccc tggtggtgga ggacgcgacg
     2281 gccagcccca ccatggggcc gttgcgcgtc atggcccagc gcgaaggctt ccgcagcatg
     2341 gtcgtcacgc cctttgtcta ccacggcaaa gccatcggcg ccctgaccct ttaccatgac
     2401 aatccgcatg agtacagcga tgacgatgtc tcggcgctga cgaccttcgc caaccatgtg
     2461 gcggtggccc tcggcaatgc gcgcctgttc gagcaggccc agcggcaggt gcgccgctcc
     2521 aacttcctgg ccgaagccgg gcggctgctc aactccagcc tcgacatggg ccatgtgctc
     2581 aactcgctca gccgctcagc ggtggaggtg ctgggcgagg cgtgcgccat ctacctgctg
     2641 cgcaagaacg aagacgaact ggcgctgacc gcctattccg atcaactcga cgaaaacatc
     2701 cgcgaccggg tcgcattcct ggaggcccac ccgccgcgcc tgggggcgac cgggatcggg
     2761 ttggccgcgc tgcgcggcga gtcgctgctg gtcgatgccc agcacccgcg gcccgatagc
     2821 ggccccgatc tctacctgga gggcctgggc gcgcattcgt acctggtggt g
//
LOCUS       DASUYC010001158         2235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_439185,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001158 DASUYC010000000
VERSION     DASUYC010001158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439185"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..1621
                     /locus_tag="VF276_18905"
     CDS             176..1621
                     /locus_tag="VF276_18905"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HEX6608978.1"
                     /translation="MLFNSIYYAFFLPVVLALYWAIPRRVRYPLLLVASYLFYMNWIP
                     AYIGLILALTVVNYGFGFLIARQRSRAWLALAVAVDLGVLGYFKYANFFIGTARDVGL
                     DFPTAHILLPLGISFFTFEFIHYVVDVWRGSPPVRNFVQFALFAAFFPTQIAGPIKRY
                     QAFVHQLQEYPRFDAALAIDGVHLIARGLIKKVILADNLARIANLGFDHPLTLATASS
                     WIAVYAFAFQIFFDFSGYTDIGRGSAQLFGFTVPENFAAPYLARNLSDFWRRWHMSLS
                     SWLRDYLFIPLGGSRGSRLETYRNLFLTMALGGMWHGAAWHFLIWGSFQGVGLAGLRW
                     LGDRGILPRKGDPFPGEAPPPSRVARWRAPIGTLLAVALTFQFTCIGWVLFRADTLAA
                     AGQMFSRMFVPVSGDPVYSQNDITLVIAVVIGYFVLVGGAGWLQRTLSGARWSTIQRT
                     AWNLRPVLYAAALVWMVVFPADAQRFIYFQF"
     gene            1638..>2235
                     /locus_tag="VF276_18910"
     CDS             1638..>2235
                     /locus_tag="VF276_18910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608979.1"
                     /translation="MAGLIETTETPPAAPERAPGGWSLIRRRLLPQALVVLALLIVVD
                     VVVAVGVEPLSLFDKSSKASTAIGRVALYHGFPTTPDVILMGNSRVQAGLDPSLLVNR
                     AKAQFQQPVTVLNLGLVGGTPQPNYWLLKNIIQSNKQPKLIIYGATEFEFNPNSKVER
                     WNYADELATLGDYGDIFPDMSRHIDQQLSFLLGRVWKLY"
BASE COUNT          325 a          765 c          678 g          467 t
ORIGIN      
        1 ggcgcttgtt tttcccttga ttctgcacta cggagctgcg aggaacacga taatcttcgc
       61 ggccttcgtg tcttcgcggt gaaactgtca acgactacgg atggcagaat ctggacacgg
      121 cccggcgcgg tcgggtataa tgggaggatt cagcgcaagg agccccggtt gccggatgtt
      181 atttaatagt atctattacg cgttcttcct gcccgtggtc ctggctctct actgggccat
      241 cccccgccgg gtgcgctacc ctctcctgct ggtcgccagc tacctgttct atatgaactg
      301 gatccccgcc tatatcgggt tgatcctggc gctcacggtg gtcaactacg gcttcggttt
      361 cctcatcgcc cggcagcgta gccgtgcctg gctggccctg gccgtcgccg tggacctggg
      421 cgtgctcggc tatttcaagt acgccaactt cttcattggc accgcgcgcg atgtggggct
      481 ggactttccg accgcccata tcctgctgcc gctgggcatt tcgttcttca ccttcgagtt
      541 catccattac gtggtcgatg tctggcgcgg cagccccccg gtgcgcaact tcgtgcagtt
      601 cgccctgttc gccgccttct tccccacgca gatcgccggc ccgatcaagc gctaccaggc
      661 cttcgtccac cagttgcagg aatatccccg cttcgatgcg gcgctagcca tcgacggggt
      721 gcatctgatc gcccgcggcc tgatcaaaaa ggtcatcctg gccgacaacc tggcgcgcat
      781 cgccaacctg ggcttcgacc acccgctgac gctcgccacg gccagttcct ggatcgcggt
      841 ctatgccttt gcgttccaga tcttcttcga cttctcgggt tataccgaca tcgggcgcgg
      901 gtcggcgcag ttgttcggct tcaccgtgcc cgagaacttc gccgcgccgt atctggcgcg
      961 caacctgtcg gatttctggc ggcgctggca catgtcgctg tcctcatggc tgcgcgacta
     1021 cctgttcatc ccgctcggcg gctcgcgtgg ttcccggctg gagacgtacc gcaacctgtt
     1081 cctgactatg gccctgggcg gcatgtggca cggcgccgcc tggcacttcc tcatctgggg
     1141 atcgttccag ggcgtggggc tggccgggct gcgctggctg ggcgaccggg gtatcctgcc
     1201 gcgcaaaggc gacccgttcc ccggcgaggc cccgccgccc agccgggtcg cccgctggcg
     1261 cgcgcccatc ggcacgctgc tggccgtggc gctcaccttc cagttcacct gcatcggctg
     1321 ggtactgttc cgcgccgata ccctggccgc cgccgggcag atgttcagcc gcatgttcgt
     1381 gccggtgagc ggcgacccgg tctacagcca gaacgacatc acgctggtga tcgcggtcgt
     1441 catcggctat ttcgtgctgg tggggggtgc cgggtggctg cagcgcacgt tgtcgggtgc
     1501 ccgctggagc accatccagc gcaccgcctg gaacctgcgc ccggtgctat atgccgccgc
     1561 gctcgtctgg atggtcgtgt tcccggcgga cgcccagcgt tttatctatt tccagttcta
     1621 gaggataccg tcgcaccatg gccggtttga tcgagacaac cgaaacgccc cccgccgcgc
     1681 ccgaacgtgc ccctggcggc tggagcctca tccgccgccg gctgctgccg caggccctgg
     1741 tggtgctggc cctgctgatc gtggtcgatg tggtggtggc cgtgggcgtc gagccgttgt
     1801 ccctgttcga taaaagctcg aaagcctcga ccgccatcgg ccgcgtggcg ctctaccacg
     1861 gctttcccac cacgcccgac gtgatcttga tgggcaactc gcgcgtgcag gcgggccttg
     1921 acccctccct gctcgtgaac cgggcgaagg cccagttcca gcagccggtc accgtgctga
     1981 acctgggcct ggtgggcggc acgccccagc ccaactactg gctgctgaaa aacatcatcc
     2041 agagcaacaa gcaacccaag ctgatcatct acggggcgac cgaattcgag ttcaacccca
     2101 actcgaaggt cgagcgttgg aactatgccg acgaactggc gacgctgggc gactatggcg
     2161 acatcttccc cgacatgagc cggcacatcg accagcaact cagcttcctg ctgggccggg
     2221 tctggaagct ctacc
//
LOCUS       DASUYC010001159         2096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_670176,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001159 DASUYC010000000
VERSION     DASUYC010001159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670176"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..1239
                     /locus_tag="VF276_18915"
     CDS             157..1239
                     /locus_tag="VF276_18915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HEX6608980.1"
                     /translation="MYTVTLIPGDGIGPEISQATRRVLDATGVPLEWDVQEAGTAVAE
                     KTGSALPDSVIESVRRNKVALKGPITTPVGKGFRSVNVGLRKALDLYANVRPCKTRPG
                     VRSRYENIDLIVVRENTEDLYAGIEFNEGAPETADLITWIAEHNGAKIRPDSAISIKP
                     ISISGSRRIVKFAFEHAKANGRRKVSAVHKANIMKATDGLYLRTAEDVAKEYPDIAFE
                     NVIVDNMCMQLVQKPELYDVLVLPNLYGDIISDLCAGLVGGLGVAPGANIGEGVALFE
                     PIHGSAPKYAGQNKVNPMAMMFSGVMMLRYLGEHAAADRVEHILDDIIAEGKYVTYDL
                     KQNRDDPTALSTSAVADEIVRRLQAA"
     gene            1321..>2096
                     /locus_tag="VF276_18920"
     CDS             1321..>2096
                     /locus_tag="VF276_18920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6608981.1"
                     /translation="MRIAVNGFFWKDPYTGSGQYLRRLWEYLPAVPESAGHEFVLLCP
                     GVPQERPPTDARRGVLVAPAGPFAGRNANLAQVWWEQIGLPAAARRVRADVLHSPYLA
                     APLAAPCPVVVTAHDLIPWVLPAYRASRALRLYLRLAQAGCRRARLVLADSEASRRDV
                     LRFFHLPPARVRTVLLGIEPEFRAPVPPEDVAGGRARFGLPPRYAFYIGGFDARKDVP
                     LLLRAWAAALPALRRAGEPAPVLAVAGRLPEPGGLYPDLPA"
BASE COUNT          318 a          724 c          734 g          320 t
ORIGIN      
        1 cagcccgcgc aggcgggctt cgtaaggcta gcccgcccct tcaggggccg ggcacgccgg
       61 tagcgcgggc cgtggctgat cggctataat ggggtgcttc atgagtgtga ggggcgggcc
      121 tgcgtccgcc tagctgtgcg aaaggacaaa gacgtcatgt ataccgtaac gctgattccg
      181 ggggacggca ttggtccgga gattagccag gcgacccggc gcgtgctcga cgccaccggc
      241 gtgccgctcg aatgggacgt gcaggaagcc gggacggcag tggccgaaaa gaccggcagc
      301 gccctgcccg actcggtgat tgagtccgtg cggcgcaaca aggtggcgct caaggggccg
      361 atcacgacgc cggtgggcaa gggcttccgc agcgtcaacg tggggctgcg caaggcgctc
      421 gatctctacg ctaacgtgcg tccctgcaag acccggcccg gcgtgcgctc gcgctacgag
      481 aacatcgatc tgatcgtcgt gcgcgagaac accgaggacc tgtatgccgg gatcgagttc
      541 aacgagggcg cgcccgaaac cgccgacctg atcacctgga tcgccgagca taacggcgcc
      601 aagatccgcc ccgactcggc catcagcatc aagccgatca gcatcagcgg cagccggcgc
      661 atcgtcaagt tcgccttcga gcatgccaag gccaatgggc ggcgcaaggt cagcgcggtg
      721 cacaaagcca acatcatgaa ggccaccgac gggctgtacc tgcgcacggc ggaagacgtg
      781 gccaaggaat accccgatat cgccttcgag aacgtgatcg tcgacaacat gtgcatgcag
      841 ttggtgcaga agcccgagct gtatgacgtg ctggtgctgc ccaacctcta cggcgacatc
      901 atctcggacc tctgcgccgg gctggtcggc gggctgggcg tggcgccggg ggccaacatc
      961 ggcgagggcg tggccctgtt cgagccgatc cacggcagcg cgcccaaata cgccgggcag
     1021 aacaaggtca acccgatggc gatgatgttc agcggcgtga tgatgctgcg ctacctgggc
     1081 gagcacgccg ccgccgaccg ggtggagcac atcctggacg atattattgc cgaaggcaag
     1141 tatgtgacct acgacctcaa gcaaaaccgc gacgacccga cggcgctcag cacctcggcg
     1201 gtcgccgacg agatcgtgcg ccgcttgcag gccgcgtaac caccgccact tcgtccggcg
     1261 gcgcggttgc ccggcatccg aatgccgggg gccgtgccgc cgcgcctgta agcgaccctc
     1321 atgcgcatcg ccgtcaacgg gtttttctgg aaggacccct ataccgggag cggccagtac
     1381 ctgcgccggc tctgggaata cttgcccgcc gtgccggaga gtgccgggca cgagttcgtg
     1441 ctgctctgcc ccggcgtgcc gcaggagcgc ccgcctacgg acgcgcgccg cggggtgctg
     1501 gtcgccccgg cggggccgtt tgccgggcgc aacgccaacc tggcccaggt gtggtgggag
     1561 cagatcggct tgcccgcggc ggcccggcgg gtgcgggccg atgtgctgca cagcccctat
     1621 ctggccgcgc cgctggccgc tccctgcccg gtcgtcgtca ccgcgcacga cctgatcccc
     1681 tgggtgctgc ccgcgtaccg cgcctcgcgc gcgttgcggc tctacctgcg cctggcccag
     1741 gccggatgcc gccgcgcccg cctggtgctg gccgactccg aagcatcccg gcgcgatgtg
     1801 ctgcgctttt tccacctgcc gcctgcgcgg gtgcgcacgg tgctgctggg catcgagccc
     1861 gagttccgcg cgccggtgcc gccggaggat gtggccgggg ggcgggcgcg cttcggcctg
     1921 ccgccccgct acgcctttta tatcggcggc ttcgacgccc gcaaggacgt gcccttgctg
     1981 ctgcgcgcct gggccgcggc gttgcccgct ctgcgccgcg ccggtgaacc ggccccggtg
     2041 ctggcggtgg ccgggcgctt gccggagccg ggtggcctct atcccgactt gcccgc
//
LOCUS       DASUYC010001160         3532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1133684, whole genome shotgun sequence.
ACCESSION   DASUYC010001160 DASUYC010000000
VERSION     DASUYC010001160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133684"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="VF276_18925"
     CDS             <1..682
                     /locus_tag="VF276_18925"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015123661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HEX6608982.1"
                     /translation="EQLKKMGSFEYHHTSFSYIDRETSEERFVGVPEEGGRDLIATDP
                     LAPGSVYTAAVDDQGRVGLYRIEVGCSPGTGKLRLAGGIDSTMKESLLRAFGYLQAHK
                     SQLGIAQLFDTTDFHVEAIDLLTNRVPADAGIAFVVAVYSALKRQPGLPALVVLGDLT
                     IQGNLKPTRSLAEPLRIAMDNGARRVLVPIENKRHFFDVAAEVVERVDPVFYSDPQVA
                     AFKALGLT"
     gene            760..>3532
                     /locus_tag="VF276_18930"
     CDS             760..>3532
                     /locus_tag="VF276_18930"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6608983.1"
                     /translation="MIILTRLQVNHFKSLQNIDLVFPRQGSILVEGLNEAGKSTLFEA
                     VYCALYGEPLVGEETGNRGRAAYDGAIRYHADAAWLRLELDVAGTLLTVERTLRAKGA
                     NRAKLTVARPGEAVEEVNTVRAVNERLLQELGNLDKEALLNSCFVEQKKLSKLEDLGT
                     TDRRRSLEHLLNLDKLQQLHDEFKLTRQDDSLLETLRRRAELAHAQAQVPVLETTLTD
                     LGRRLMLVSIHEALARTAQHTANRDELARQQAALTAEREQVQGVQRRITALKRLQPAL
                     VEVTGFRAQVTQATMRLEETTAALAELDRQEQVELPALRDRQQRLQTLDALLATRQQR
                     TSDLQRTEREIAARQADLDAIDRRTALAAELSRKIADMQVRIDRQRAAISADEAAALA
                     QKAEATARQAALQQVAAQFDPLRDAEYARQQAAQALARAVDQAADYAALQDRLQTAAD
                     TARADEAAANQANEAYAQAQQVAEQIRTLVATQQAREQDHRAAVVAVTDAERALAHAQ
                     EEVTRQEHIHAAIQQTLATLEIREDALRAAIADHDAARQARIEIAGLMNDQQQREQEY
                     THAQAAATQAAATAAAATEWRVAFDAAVAGQEARATEVQAAETAHTAAQQALTTARTV
                     RALTAWLQARRAEEALARGTAAHPAEEGAVDAAETQLAAQTRSRAAAQRRVLGLAAAG
                     GALILTGIALLVLNLSMPAGITLVLLGLGLGAAVLRAARGGARVQQEVVAAQQAVETA
                     RRHVAQVAAERKAAEALGGGAARLAEAAAELRALDVPIPASIVDAETRLAADGMSGDV
                     RAAEAEVTRCRTVLDAARARLHESTLKYSLLKNQEANHAGSIDPSDLKGQVATLAAAV
                     QTGRETLATRAQILFPPDLGAPEDWAAPAHDGTHRAALDDLVETRRAALDAARQAQTA
                     AQTALEVLAR"
BASE COUNT          664 a         1208 c         1192 g          468 t
ORIGIN      
        1 ggaacaactc aagaagatgg ggtcctttga gtaccatcac acctcgtttt cctacatcga
       61 ccgggaaacc agcgaagaac gctttgtcgg cgtgcccgaa gaggggggcc gcgacctgat
      121 cgccaccgat ccgctggcgc ccggctcggt ctacaccgcc gccgtggacg atcagggccg
      181 cgtggggctt taccgcatcg aggtcggctg ctcgccgggc acgggcaagc tcagactggc
      241 cggcggcatc gacagtacga tgaaagaatc gttgctgcgc gccttcggct acctgcaagc
      301 gcacaaaagc cagttgggca ttgcccaact cttcgatacg accgacttcc acgtagaagc
      361 catcgatctg ctcaccaacc gggtgcccgc ggatgcgggg atcgccttcg tggtcgccgt
      421 ctactcggcc ctgaagcgcc agccggggct gcccgccctg gtcgttctcg gcgacctgac
      481 catccagggc aatctcaaac ccacccgcag cctggccgaa ccgctgcgca tcgccatgga
      541 caacggcgcg cgccgggtgc tggtgcccat cgagaacaag cgccacttct tcgatgtcgc
      601 cgccgaagtt gtggagcgag tggatccggt cttctacagc gatccccagg tggcggcgtt
      661 caaggcgctg gggctgacct gatacacccc gcgctcacat agcagtctca cgctatcggc
      721 tttacgacct tagtctctac caacccagga gccgcggcca tgattatcct cacccgcctg
      781 caggtgaacc actttaaatc gctacaaaac atcgatctgg tgtttccgcg ccagggcagc
      841 atcctcgtcg aaggcctgaa cgaggccggc aaatcaaccc tcttcgaggc cgtctactgt
      901 gcgctgtacg gcgagccgct ggtgggcgaa gagaccggga accggggccg tgccgcctat
      961 gacggcgcga tccgctatca cgccgacgcg gcgtggctgc gtctggaact tgatgttgcc
     1021 ggtaccctgc tcacggtgga gcggacgctg cgggccaagg gcgcgaaccg ggccaagctg
     1081 accgtggccc gccccggcga agcggtggaa gaggtcaaca ccgtgcgtgc cgtgaacgaa
     1141 cggctgttgc aggaattggg caacctggac aaagaggcgc tgctcaactc ctgttttgtc
     1201 gagcaaaaga aactctcgaa gctggaggac ctgggcacca cggatcgccg ccgctccctg
     1261 gagcatttgc tgaacctgga taagctccag caattgcacg acgaattcaa actcacccgc
     1321 caggacgaca gcctgctgga gacgctgcgc cgccgcgccg aactggcgca tgcccaggcc
     1381 caggtgcccg tgctcgagac gaccctgacc gacctgggcc ggcgcctgat gctggtgtcc
     1441 atccacgaag cgctggcgcg gaccgcccag cataccgcga atcgcgacga gctagcccgg
     1501 caacaggccg cgctgaccgc ggagcgcgaa caggtccagg gcgtgcagcg ccggatcacg
     1561 gccctgaaac ggctgcaacc ggcgctggtc gaggtgacgg gattccgcgc gcaggttaca
     1621 caagccacca tgcggttgga ggaaaccaca gcggctctcg cggagctgga tcgccaggag
     1681 caagtggagt taccggcatt gcgcgaccgg cagcaacggc tccaaaccct cgatgccctg
     1741 ctggcgaccc gacagcagcg gacctcggat ctccagcgca ccgagcggga gatcgcggcg
     1801 cggcaggcgg acctggatgc gattgaccgc cgtaccgcac tcgccgcgga gctaagccgg
     1861 aagatcgccg acatgcaagt gcgaatcgac cggcaacgtg cggccatcag cgccgatgag
     1921 gcggcggcgc tggcgcagaa agcggaggcg acggcgcgcc aggcggcgct gcagcaggtg
     1981 gcggcgcagt ttgatccgct ccgcgacgcg gaatacgccc ggcagcaggc cgcgcaggcc
     2041 ctggcgcgcg ccgtggatca ggccgcggac tacgcggccc tacaggatcg gttgcagacg
     2101 gcggctgaca cggcccgtgc agacgaagca gctgctaacc aggcgaacga agcctatgcg
     2161 caggcccagc aagtagccga gcaaattcgc acgctagtcg ccacgcagca agcacgggag
     2221 caagatcacc gggcagcggt cgtggccgtc acggatgcgg aacgcgccct cgcccacgcc
     2281 caggaagaag tcacccgtca ggagcacatc cacgcggcga tccagcagac acttgctacg
     2341 ttggagatac gcgaagacgc gttgcgcgcg gccatagcag atcacgacgc cgcccggcag
     2401 gcgcggatcg agatcgccgg gttgatgaac gaccagcagc agcgcgagca ggagtatacc
     2461 cacgcccagg cagcggcgac ccaggccgcc gccaccgccg cggcagccac cgagtggcgc
     2521 gtcgcgtttg atgcggcggt cgccggccag gaggcgcggg ccacggaggt gcaagccgcc
     2581 gaaacggccc atacggcggc ccagcaggcg ctcacaacgg cgcgcacagt ccgcgcgttg
     2641 accgcctggc tgcaggcccg gcgggccgaa gaggccctgg cgcgcgggac agccgcccac
     2701 ccagcggagg aaggcgcggt ggatgctgcc gaaacccaac tggcagctca gacccggagc
     2761 cgcgctgccg cccaacgccg ggtgctgggc ctcgcggcgg ccggcggcgc gctgatcctc
     2821 accggaatcg ccttgctcgt gctgaacctg agcatgccgg ccgggatcac cctggtgctg
     2881 cttgggctgg gtctgggcgc tgcagtgttg cgggcggcgc gcggaggggc gcgcgttcag
     2941 caagaagtgg tggccgctca acaggcagtg gagacagccc gccgccatgt ggcgcaggtc
     3001 gccgccgagc ggaaggctgc cgaagcccta ggtgggggcg cggcccgctt ggccgaagcc
     3061 gcagccgagt tgcgggcgct ggatgtgccc atccccgcca gcatcgtgga cgccgaaacc
     3121 cggctggcgg cggacggcat gtccggggat gttcgggcgg ccgaagccga agtaacccgc
     3181 tgccggaccg tcttagacgc agcgcgcgcc cgcctgcacg aaagcacgct caaatatagc
     3241 ctgctcaaga accaggaagc gaaccacgcc ggatccatcg atccctctga tctgaagggg
     3301 caggtggcga ccctggccgc cgccgtgcaa acaggccggg aaaccctggc aacacgcgca
     3361 cagatactat ttccgcccga tctgggcgcg cccgaggact gggcagcgcc cgcgcacgac
     3421 gggacacacc gcgccgccct cgatgatctg gtcgaaaccc ggcgcgcggc gctagacgct
     3481 gcccgccagg cgcagaccgc cgcgcagacg gccctggagg tcctggctcg cc
//
LOCUS       DASUYC010001161         5029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_439721,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001161 DASUYC010000000
VERSION     DASUYC010001161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5029
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439721"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VF276_18935"
     CDS             <1..358
                     /locus_tag="VF276_18935"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6608984.1"
                     /translation="GADADNSYATVMAVNVATLPEGKQFIADYKAAFNDDPGAYSASA
                     FDATNIIIQAIKTAGKKDREAVRAAIAATKDYKGVLGTTGFDDNGDTTNRWVSIYQVK
                     GGKWEFIDQQRFEAAK"
     gene            529..1470
                     /locus_tag="VF276_18940"
     CDS             529..1470
                     /locus_tag="VF276_18940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX6608985.1"
                     /translation="MDPVFILQQLINGISRGSVYALIALGYTMVYGIIELINFAHGDV
                     FMLGSFTAISVVAVFGVNDANPVLQDPLMVVVVLLVTFIVTMVLMGLLGVVVEQVAYR
                     PLRNAPKLAPLISAIGVSYILQNVGQIWRGPAPVNFPDIFQDTFYRIPIGTQALTIRS
                     KAILILVVAIILMVLLTLFVQRTKMGKAMRATALDRETAQLMGINVNMTIALTFFIGA
                     ALAGAGGLLYGMFIRAVQFTLGYQAGLRAFTAAVLGGIGNIPGAMLGGFLIGILEAMS
                     DTFFQPQWTQAVVFAVLILILVFRPSGLLGQHVPEKV"
     gene            1478..2704
                     /locus_tag="VF276_18945"
     CDS             1478..2704
                     /locus_tag="VF276_18945"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX6608986.1"
                     /translation="MSQSTSARPSPGVANKSLLPPARRASRRSMITAGILGVLLLLVP
                     VPQLLAENHIVTLPFSQLYNQAIINAAADTGAFVLLALGLNIVVGYAGLLDLGYAAFF
                     AIGTYTYAILGSSLFDIHIPFWIMLFVSAAITALFGVAFGAPTLRLRGDYLAIVTLGF
                     GEIVPTFILNLDQLTGGTNGISGVDRPAIGGFIIGAQNDNISFYYLALILVAGIIFVV
                     NRLRDSRLGRAWMAIREDELAAATMGINTTTTKLFAFGLGAAVSGFAGTFYAAHLGFA
                     SPQQFNFSQSVLILCMVILGGMGNLWGVILGAVVIYLLQTVFLIQITNWINDLGRAIN
                     FDLLVQIKLTDYTFFIYGVVLVLFMLFRPEGLIPSSRRREELRPETEDISEAERQTVY
                     DVREAGQADPDTQGGD"
     gene            2810..3553
                     /locus_tag="VF276_18950"
     CDS             2810..3553
                     /locus_tag="VF276_18950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6608987.1"
                     /translation="MRFGGLVAVNNVDFTIPRGGIVSLIGPNGAGKTTFFNTITGLYK
                     PTSGRMIFDGKDITGTPPSAITALGIARTFQNIRLFANMTALENVLVGQHCRLRSGLL
                     GIVVRTPAVRQEETEAREKAVRLLKFVRLTGAADDLAKNLPYGDQRRLEIARALASNP
                     KLLLLDEPTAGMNPQETVSLISFIRELRQQLEIAILLIEHDMKVVMGLSERISVLDYG
                     VKIAEGTPAEIRANPRVIEAYLGRGSTSE"
     gene            3591..4391
                     /locus_tag="VF276_18955"
     CDS             3591..4391
                     /locus_tag="VF276_18955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6608988.1"
                     /translation="MDETAPYRPLLLRAVGITKRFGDLVALDGIDLVIPRHAIISLIG
                     PNGAGKTVFFDVLSGIHKPTSGLIWFDGQAIAGLAPDRIAQLGIGRTFQNIRVFANMS
                     VRENVLVGCHCRMRGGVWDAVFHTPIQIEDETVAAARVRDLLAFTGLTSVAEMPAKHL
                     SYGDQRRLEIARALATDPALLLLDEPSAGMHPQETAAIMALIGRLRDERGVTILLIEH
                     DMRLVMRISDRVAVLDYGVKIAEGAPAQVRADRRVIEAYLGRPVPVGV"
     gene            4407..>5029
                     /locus_tag="VF276_18960"
     CDS             4407..>5029
                     /locus_tag="VF276_18960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6608989.1"
                     /translation="MALLEVDHIETYYGNIRALKGVSLTVNKGEIVTLIGSNGAGKTT
                     TLRTISGMLHPRRGQVRLEGKRIDTLPADQMVRLGLAHSPEGRRIFRRMTVQENLEMG
                     AFARNDKAGIAADLERVLGLFPRLKERLAQQGGTLSGGEQQMLAMGRALMSKPTILLL
                     DEPSMGLAPILVETIFGIVKDLNAAGNTILLVEQNALLALQIAHRGYV"
BASE COUNT          878 a         1711 c         1583 g          857 t
ORIGIN      
        1 cggagccgat gccgacaaca gctacgccac ggtgatggcg gtgaacgtcg cgactctgcc
       61 ggaaggcaag cagtttattg ccgactataa agccgccttc aacgacgatc ccggcgccta
      121 cagcgccagc gccttcgacg cgaccaacat catcatccag gccatcaaga cggcgggcaa
      181 gaaggaccgc gaagccgtcc gcgcggccat cgcggcgacg aaggattaca aaggcgtgct
      241 gggcaccacc ggcttcgacg acaacggcga caccaccaac cgctgggtct cgatctacca
      301 ggtcaagggc ggcaagtggg agtttatcga ccagcagcgc tttgaggcgg ccaaataaga
      361 cggagtacag aaccgggggc gggcctgccc caccggcgcg gcgcggggac ctcccagccc
      421 cccgccgccc aggggcagga ggccgcccga cgatcacgca aggggcgcgt ctagcctgag
      481 gccgcccctt attacacccc ggcaccaggg ctggagagga gacgcgtatt ggacccagtt
      541 ttcatattgc aacaactgat taacgggatc tcgcgcggat cggtgtatgc gctcatcgcg
      601 ctcggctaca ccatggtcta cggcatcatc gaactgatca acttcgccca cggcgatgtg
      661 ttcatgctcg gctccttcac ggccatcagc gtcgtcgcgg tgttcggggt gaacgacgcc
      721 aatcccgtgc tccaggaccc gctcatggtc gtcgtggtcc tgctggtcac gtttatcgtc
      781 accatggtgc tgatgggcct gctgggagtg gtggtcgaac aggtggccta ccggccctta
      841 cgcaacgcgc caaaattggc gcccctgatc tcggctatcg gcgtgtcgta tatcctgcaa
      901 aacgtcggcc agatctggcg cggccccgct ccggtcaact tccccgacat cttccaggac
      961 acgttctacc gcatccccat cggcacccag gccctcacca ttcgcagcaa agccatcctg
     1021 atcctggtcg tcgccatcat cctgatggtg ttgctgacgc tgttcgtgca gcggaccaag
     1081 atgggcaagg ccatgcgggc caccgcgctg gaccgggaga cggcgcaact catgggcatc
     1141 aacgtgaaca tgaccatcgc gctcaccttc ttcattgggg cggcgctggc cggggccggc
     1201 gggctgctct acggcatgtt catccgcgcg gtgcagttca ccctgggcta ccaggccggg
     1261 ttgcgcgcct ttaccgccgc cgtgctcggc ggcatcggca atatccccgg cgccatgctc
     1321 ggcggcttcc tgatcggcat tctcgaagcc atgagcgaca cgttcttcca gccgcaatgg
     1381 acgcaggccg tcgtcttcgc ggtgctgatt ctcattctcg tcttccgacc gtccgggctg
     1441 ctcggccagc acgtcccgga gaaagtgtag gcgcacgatg tcacaatcca caagcgcccg
     1501 gcccagcccc ggtgtagcca ataagagttt gctgccgccc gcgcggcggg ccagccgccg
     1561 ctcgatgatc acggcaggca tcctgggcgt cctgcttcta ctggtgccgg tgccgcaact
     1621 gctggccgag aaccatatcg tgaccctgcc gttctcccag ctctacaacc aggcgattat
     1681 caacgcggcc gccgataccg gggccttcgt cctgctcgcg ctcggcctga acatcgtggt
     1741 aggctatgcc ggcctgctcg acctgggcta cgcggcgttc ttcgccatcg gcacctatac
     1801 ctacgccatc ctggggtcgt cgctcttcga catccacatc cccttctgga tcatgctctt
     1861 cgtgtccgcc gccatcaccg cgctcttcgg cgtggccttc ggcgccccga ccttgcgctt
     1921 gcgcggcgac tacctggcga tcgtcaccct gggcttcggc gagatcgtgc cgacctttat
     1981 cctcaacctc gaccaactga ccggcggcac caacggcatc tccggggtgg atcgcccggc
     2041 catcggcggc ttcatcatcg gcgcccaaaa cgacaacatc agcttctact acctggccct
     2101 cattctggtc gcgggcatca tcttcgtggt caatcgcctg cgcgactcgc ggctgggccg
     2161 ggcgtggatg gcgatccgcg aggacgaact ggccgccgcg acgatgggca tcaacacgac
     2221 gacgaccaag ttgttcgcct tcgggctggg cgccgccgtc tcgggcttcg cgggcacctt
     2281 ctacgcggcc cacctcggct tcgcctcgcc ccagcagttc aacttcagcc aatcggtgct
     2341 gatcctctgc atggttatcc tgggcggcat gggcaacctg tggggcgtca tcctcggcgc
     2401 cgtcgtcatc tacctcttgc agacggtctt cctgatccag atcaccaact ggatcaatga
     2461 cctggggcgg gcgatcaact tcgacctgct ggtccagata aagctgaccg actatacctt
     2521 cttcatctac ggcgtggtgc tggtgttgtt catgttgttc cggcccgaag gcttgatacc
     2581 cagcagccgg cgccgcgaag aactgcgtcc ggagaccgag gacatcagcg aggccgaacg
     2641 ccagacagta tacgatgtgc gcgaagccgg gcaggctgac cccgataccc aaggagggga
     2701 ctaaacgatg agccaaccag gtagcagcct gcccaccgcg gccgcgcccc cggcgggcgg
     2761 gcctgccgtc gccgatggga aggatttgct ctcggcccaa aacatcacca tgcgcttcgg
     2821 gggcctcgtg gcggtcaaca acgtcgattt caccatcccg cgcggcggca tcgtcagcct
     2881 gatcgggccg aacggcgcgg gcaaaacgac cttcttcaac acgatcaccg ggctctataa
     2941 gcccaccagc gggcgcatga tcttcgacgg gaaagacatc accggcacgc cgccgtcggc
     3001 catcaccgcg ctgggcatcg cccgcacgtt ccagaatatc cggctcttcg ccaatatgac
     3061 ggccctggaa aacgtgctgg tgggccagca ctgccgcctg cgctcgggcc tgctgggcat
     3121 tgtcgtgcgc acgcccgccg tgcgccagga ggagaccgag gcccgcgaga aagccgtgcg
     3181 cctgctcaag ttcgtgcgcc tgaccggcgc cgccgacgac ctggcgaaaa acctgcccta
     3241 tggcgaccag cgccgcctgg agatcgcccg cgccctcgcc agcaacccca agctgttgct
     3301 gctcgacgag ccgaccgccg ggatgaaccc ccaggaaacg gtgagcctga tcagcttcat
     3361 ccgcgagttg cgccagcagt tggagatcgc catcctgctg atcgagcacg atatgaaggt
     3421 cgttatgggc ctgtcggagc gcatctccgt gctggactac ggcgtgaaga tcgccgaggg
     3481 cacgcccgcc gagatccgcg ccaacccgcg ggtgatcgag gcgtacctgg gccgggggag
     3541 cacgagcgaa taaggcgctg ccgatccgcg gccaggaagc agaggcagcc atggacgaga
     3601 ccgcccccta ccgcccgctg ctgttgcggg cggtcgggat cactaaacgc ttcggcgacc
     3661 tggtggccct cgacgggatc gacctggtga tcccgcgcca tgcgattatc agcctgattg
     3721 gccccaacgg cgcgggcaaa accgtgttct tcgacgtgct gagcggcatt cataagccga
     3781 ccagcggcct gatctggttc gacggccagg cgattgccgg gctggcgccc gatcgcatcg
     3841 cccagttggg catcgggcgg acctttcaga acatccgcgt gttcgccaac atgtcggtgc
     3901 gcgaaaatgt gctggtgggc tgtcattgcc gcatgcgcgg cggggtgtgg gacgccgtct
     3961 ttcacacccc gatccagatc gaggacgaga cggtcgccgc ggcacgggtc cgggacctgc
     4021 tggcctttac cggcctgacg agcgtggcgg agatgccggc caagcacctg tcgtatggcg
     4081 atcaacgccg cctggagatc gcccgcgccc tggcgaccga tccggcgctg ctgctgctcg
     4141 acgagccgag cgccggtatg cacccgcagg aaacggcggc gatcatggcg ctcatcgggc
     4201 gcttgcgcga cgagcgcggg gtgacgattc tgttgatcga gcacgacatg cgcctggtca
     4261 tgcgtatctc cgaccgggtg gccgtgctcg attacggcgt gaagatcgcc gagggagcgc
     4321 ccgcccaggt gcgggccgac cggcgcgtga tcgaggcata tctgggccgg ccggtgccgg
     4381 tgggcgtcta agcagaggag caagggatgg cattactcga agtcgaccac atcgaaacct
     4441 actacggcaa cattcgcgcg ctcaagggcg tctccctgac cgtgaacaag ggcgagatcg
     4501 tgaccctgat cggcagcaac ggcgccggga agacgaccac gctgcgcacg atttcgggga
     4561 tgctccaccc gcgccgcggg caggtgcggc tggaaggcaa acgcatcgac acgctgcccg
     4621 ccgaccagat ggtccgcctg ggcctggctc attcgccgga aggccggcgc atcttccgcc
     4681 gcatgacggt gcaggagaac ctggagatgg gcgccttcgc gcgcaacgac aaggccggca
     4741 ttgccgcgga cctggagcgc gtgctcggcc tcttcccccg gctcaaggag cggctggcgc
     4801 agcaaggcgg caccctctcc ggcggcgagc agcagatgct ggcgatgggc cgggccctga
     4861 tgagcaagcc cacgatcctg ctgctggacg agccctcgat gggcctggcg ccgatcctgg
     4921 tcgagacgat cttcggcatc gtcaaggatc tgaacgccgc gggcaacacc attttgctgg
     4981 tcgagcagaa cgccctgctg gcgctgcaaa tcgcccaccg gggctacgt
//
LOCUS       DASUYC010001162         2399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_208950,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001162 DASUYC010000000
VERSION     DASUYC010001162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208950"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..839
                     /locus_tag="VF276_18965"
     CDS             183..839
                     /locus_tag="VF276_18965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015160509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEX6608990.1"
                     /translation="MATGVNENDVVAEGKETGRLEAFSDGVFAIAITLLVLDIHVPPL
                     DENGQAGQLAPALLHQWPVYFAYVTSFLTVLIMWTNHHALFRHIARVNQPFLMLNGLL
                     LMGITLVPFPTALVSEYMGAPDAHVAGVVFGGTFLALACVFNLLWRYASTDNRLLARD
                     ADSVQVGNISRQYAFGPPLYLACCLLALVSVPAMLVLYVLIALFFALPGLAPRLPRRA
                     "
     gene            1080..1466
                     /locus_tag="VF276_18970"
     CDS             1080..1466
                     /locus_tag="VF276_18970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608991.1"
                     /translation="MSRPRPGGVAVCRDCGAPLTLADLGFAYCAECQWQHLFHAAVEA
                     AGTHGTGIRFVFYRPAGAGDEVLDSTSQPWRAFSGDPPAVCAFCGGPVAQGWVHDPPG
                     HPAVHICAEHVDLIPGPPIRPAIWPG"
     gene            1662..>2399
                     /locus_tag="VF276_18975"
     CDS             1662..>2399
                     /locus_tag="VF276_18975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6608992.1"
                     /translation="MKQRLRSMLLVSAGMVLGMLLTLTWHTYGSLAQSGGCQTFPQTN
                     HKVCGKFLTYWQQHGGLAQQGYPLSEEFVETSDLNGKPYTVQYFERAVFELHPEYAGT
                     PYEVLLSQLGTYLGRSNYPQGFPAVSGQQPFYEIRNAAPADLLKSYYNAINRKEYERA
                     YSYFQGAPHPSPDVAPPYAQFVQGYADTTAVTLAVGQAMIDAGAGNVYASMPVVLTAQ
                     HTDNSTHLYAGCYFLHHVNPGISQNPLD"
BASE COUNT          361 a          890 c          722 g          426 t
ORIGIN      
        1 attgtagggg ccggtttcac accggcccgt cgcctcggat cgcggtgtag gggccgattt
       61 gaaaccggcc cgtggcctca tcgcggtgta ggggccggtt gcaatccgcc ccgccgcctc
      121 ggatcgcggt gtaggggccg gtttcaaacc ggcccgtggc cttttaccag ggaggcagca
      181 cattggcaac cggtgtgaat gaaaacgacg tagtcgccga gggcaaggaa accgggcggc
      241 tcgaagcctt cagcgacggt gtgttcgcca tcgccatcac gctgctggtg ctcgatatcc
      301 acgtcccgcc gttggacgaa aacgggcagg cggggcaact tgccccggcc ctgctgcacc
      361 agtggccggt ctacttcgcc tacgtgacca gcttcctgac ggtgctgatc atgtggacca
      421 accaccacgc cctcttccgg cacatcgcgc gtgtgaacca gcccttcctg atgctcaacg
      481 gcctgctgct gatgggcatc acgctggtgc cgttcccgac cgcgctggtc tcggagtata
      541 tgggtgcccc cgacgcccac gtggcgggcg tcgtgttcgg cggcacgttc ctggccctgg
      601 cctgcgtctt caacctgctg tggcgctatg cctccaccga caaccgcctg ctggcccgcg
      661 acgccgactc ggtccaggta ggcaacatct cgcggcaata cgccttcggc ccgccgctct
      721 atctcgcctg ctgcctgctg gccctggtga gcgtcccggc catgctggtg ctgtacgtcc
      781 tgatcgcgct cttcttcgcg ctgcccggcc tggccccgcg cctgccgcgc cgcgcctgac
      841 acggcatgcg ttcctaaatt caccgcgaag acgcgaagga cacgaagatt ttttgttttt
      901 attcttcgcg tccttcgcgt cttcgcggtg cctaatagcc ggagttgcgc ggatgtcggg
      961 gccgccaggt tgccttcgga cgccgattcg agtatcatct tttctatgga aagggtctgc
     1021 cgccggtcgc gattcctggg ccggcccggt gcgcgccggg ctgcggggag gcccgcgccg
     1081 tgagtcgccc ccggcccggc ggcgtggccg tgtgccgcga ctgcggcgcg ccgctcaccc
     1141 tggccgacct cggctttgcc tactgcgccg agtgccagtg gcagcacctc ttccatgccg
     1201 cggtcgaagc cgcgggcact catggcaccg gcatccgctt cgtcttctac cgccccgccg
     1261 gcgccgggga cgaggtgctg gacagcacca gccagccctg gcgtgccttc agcggcgacc
     1321 cgcccgccgt ctgcgcgttt tgcggcggcc ccgtcgccca gggctgggtc cacgacccgc
     1381 ccggccaccc cgccgtccac atctgcgccg agcacgtcga cctcatcccc ggcccgccca
     1441 tccgccccgc catctggccc ggctgatctt ccccccgcgc aaccaccccc gccgctcacg
     1501 cgtcgtatga tcaggcacct ccgcgccgcc gtaactcgcc gcgcccgccg tgccatagcc
     1561 tgtatgacgg ctggcactag ccccgttttt gcgcggcccg tgcggacgga tgtatagtta
     1621 tagagtcgcg gcctttggga catacgataa ggagaagtat gatgaagcag cgtttacggt
     1681 cgatgctcct ggtaagcgcc gggatggtcc tcggcatgtt gctcaccctc acctggcaca
     1741 cctacggctc gctggcccaa tcgggcggct gccagacctt cccgcagacc aaccacaagg
     1801 tctgcggcaa gttcctcacc tactggcagc aacacggcgg cctggcccag cagggctatc
     1861 cgctcagcga ggaattcgtg gagaccagcg acctcaacgg caagccctac acggtgcagt
     1921 atttcgagcg cgccgtgttc gagctacacc ccgaatacgc cggcacgccc tacgaagtac
     1981 tgctgagcca gctcggcacc tacctgggcc gcagcaacta tccgcagggc ttccccgccg
     2041 tctccggcca gcagccgttc tacgagatcc gcaacgccgc gcccgccgac ctgctgaagt
     2101 cgtactacaa cgcgattaac cgcaaggagt acgagcgcgc ctacagctac ttccagggcg
     2161 cgccccaccc gtcccccgac gtggccccgc cctacgcgca gtttgtccag ggctatgccg
     2221 acaccaccgc cgtcacgctg gccgtgggtc aggcgatgat cgacgcgggc gccgggaacg
     2281 tctacgccag catgccggtc gtgctgacgg cccagcacac cgacaacagc acccaccttt
     2341 acgcgggctg ctacttcctg caccacgtca accccggcat ctcgcagaat ccactggac
//
LOCUS       DASUYC010001163         2126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_872973,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001163 DASUYC010000000
VERSION     DASUYC010001163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872973"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="VF276_18980"
     CDS             <1..883
                     /locus_tag="VF276_18980"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6608993.1"
                     /translation="KKASDEFLIAFVGPLTSLILAGVFFGLEQIVTPTIGHVGAGAAA
                     VLQYLAFINFILALFNLIPGFPLDGGRVLRSIIWGVTRNFQRSTQLAGFVGQLVAYGF
                     IFLGLYQTFFLGDFGGGLWLAFIGWFLLNAAQQSVSGTVMRETLRGITAGQVMEPAPP
                     VAAPHMTIAQLLTQFILPYNARAVPVADDGRLVGIVTLGDIKDVPQDEWGMVTVGQRM
                     TGPDKLKVVRPQDDLQRALELINEGDFDQVPVVDATGRLVGLLTRAHVLQWLQIRDQL
                     KMQQQAPGPGRGAMPGA"
     gene            complement(1195..>2126)
                     /locus_tag="VF276_18985"
     CDS             complement(1195..>2126)
                     /locus_tag="VF276_18985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX6608994.1"
                     /translation="TAVQELAFTLADGLAYVEAAIARGLNVDDFAPRLSFFFNAHNDF
                     FEEIAKYRAARRIWAREMKERFGAKNPRSMWLRFHTQTAGQSLTAQQPEINLIRTTIQ
                     ALAGVLGGTQSLHTNSMDEAIALPSDKAVRLALRTQQIIAEESGVTNTIDPLGGSYFV
                     EALTNEMERQTYEYFDRINALGGVIPAIEANFFQDEIARASYEYQKAVDARTKTVVGV
                     NKYVQDEPLEIPILEIDPQGEEHQRARLAQLRRERDPERYTAALEHVRRAAEGTENLM
                     PALIEAAHAYATLGEITGVLRSVFGVQQFSTVV"
BASE COUNT          330 a          714 c          700 g          382 t
ORIGIN      
        1 gaaaaaggct agcgacgaat tcctgatcgc gtttgttgga ccgctcacca gcctgatact
       61 ggccggggtc ttcttcggcc tggaacagat cgtcacgccc accatcggac atgtaggggc
      121 gggcgcggcg gcggtgctgc aatacctggc cttcatcaac ttcatcctgg ccctgttcaa
      181 cctgatcccc ggcttcccgc tggacggcgg gcgggtgctg cgctcgatca tctggggcgt
      241 cacgcgcaac ttccagcggt ccacccaact ggccgggttc gtcgggcagt tggtggccta
      301 cggctttatc ttcctcggcc tctaccagac gttcttcctg ggcgacttcg gcggcgggct
      361 gtggctggcg tttatcggct ggttcctgct gaacgcggcc cagcagagcg tctccggcac
      421 cgtgatgcgc gagaccctgc gcggcatcac cgcgggccag gtgatggagc cggccccgcc
      481 ggtcgccgcg ccgcatatga ccatcgccca actgctgacc cagttcatcc tgccctataa
      541 cgcccgcgcc gtgccggtgg ccgacgatgg tcgcctggtc ggcatcgtca ccctgggcga
      601 catcaaggac gtgccgcaag acgagtgggg catggtcacg gtgggccagc gcatgaccgg
      661 cccggacaag ctcaaggtgg tgcgcccgca agacgatctc cagcgcgcgc tggaactgat
      721 caacgagggc gacttcgacc aggtgccggt ggtggacgcc acggggcgcc tcgtcggcct
      781 cctgacccgc gcccatgtgc tgcaatggct gcaaatccgc gaccaactca agatgcagca
      841 gcaggcgccg ggaccgggcc gcggcgcgat gccgggggca taaaattggg gccggggaag
      901 cacttccccg gccctttttc atccaaggct tgtcactctg agcgcagcga agagtctcgg
      961 cttttgcccg gaagagacga gattcttcgc tgcgctcaga atgacggttc ctgaaggact
     1021 tgtcactctg agcgcagcga agagtctcgg ctcctggcgg gcggcgggcc ggtttcaaac
     1081 cggcccctac acctgcgcgg tcaggccggt ttgaaaccgg cccctacaac gctcgccggg
     1141 cggcaggccg gtttcaaacc ggcccctaca ccacgaaatc accgtggccg gcgactaaac
     1201 caccgtgctg aactgctgca cgccgaacac gctgcgcagc acgccggtga tttcgcccag
     1261 cgtggcgtag gcgtgggccg cctcgatcag ggcgggcatc aggttctcgg tgccctcggc
     1321 ggcgcggcgg acgtgctcca gggcggcggt gtagcgttcc gggtcgcgct cgcggcgcaa
     1381 ttgggccaga cgggcgcgct ggtgctcctc gccctgcggg tcgatctcca gaatcgggat
     1441 ctccagcggc tcgtcctgca catacttgtt cacgccgacg accgtcttgg tgcgcgcatc
     1501 gaccgccttc tggtactcat aggaggcccg cgcgatctcg tcctggaaga agttggcctc
     1561 gatggccggg atcaccccgc ccagcgcgtt gatgcggtcg aagtattcgt aggtctggcg
     1621 ctccatctcg ttggtcagcg cctccacgaa gtacgagccg cccagcggat cgatcgtgtt
     1681 ggtcacgccg ctctcctcgg cgatgatctg ctgcgtgcgc aaggccaggc gtaccgcctt
     1741 gtcggacggc agggcgatgg cctcgtccat cgagttggtg tgcagcgact gcgtgccacc
     1801 gagcacgccc gccagcgcct ggatcgtcgt gcggatcagg ttgatctcgg gctgctgggc
     1861 cgtgagcgac tggcccgccg tctgcgtgtg gaagcgcagc cacatcgagc gggggttctt
     1921 cgcgccgaag cgctccttca tctcgcgcgc ccagatgcgc cgcgccgccc gatatttggc
     1981 gatctcctcg aaaaagtcgt tgtgcgcgtt gaagaagaag ctcaggcgcg gggcgaagtc
     2041 atccacattc agcccgcgcg cgattgccgc ctcgacatag gccagcccgt cggccagcgt
     2101 gaaggccagt tcctgcaccg ccgtcg
//
LOCUS       DASUYC010001164         3831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_2058206, whole genome shotgun sequence.
ACCESSION   DASUYC010001164 DASUYC010000000
VERSION     DASUYC010001164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2058206"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..1503
                     /locus_tag="VF276_18990"
     CDS             169..1503
                     /locus_tag="VF276_18990"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HEX6608995.1"
                     /translation="MGVQPLTRPAPRDPAEGMPFPAPGIDGLPDEALPATEAEARPPL
                     EEALPGAWHDTPHGRCFAIEYRYTLDHQHGSVPLAQLLDLPGETLSHLGRNGDFAGVD
                     RRRLLFFDTETTGLAGGTGTYVFLAGLGFFDGDSFVVRQLFLPDLQSERALLHAFNDL
                     LGRSACLVSFNGRAFDWPLIESRFTLSRMRPVQGAPLHLDLLAPARRVWKDRLPSCAL
                     GSLEAHALHFRRRGDVPGWLIPSLYFQYLRGGGINALLPVFEHNRLDVLSLLALAGYM
                     GSLLHAPDAANLESAECYGLARLYEDLDNYEAAVRCYERALKGAISPALRALTLQRLP
                     MAHKKLSHHHEALRIWEELVARETTLVFPYIELAKHYEHHTREYAQAVALVNHALALC
                     QDPWVRATLPRNTGPDLERRRVRLETKIARAATGPSPKNRGRAAEGCAEAVL"
     gene            1748..2281
                     /locus_tag="VF276_18995"
     CDS             1748..2281
                     /locus_tag="VF276_18995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017746386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEX6608996.1"
                     /translation="MDAIFDIDWRRLFVPELPILEILIRGTVIYLGIFILLRLVLKRE
                     TGTLGITDLLVVVFLADAAQNAMAGDYHSITEGLILVGTIVFWSYALSWLGYRFPRVQ
                     RFINPPPLLLVKEGRMIPRNLRQELLTSDELMSQLRQQGVRDLAEVKEAFMEGDGRIS
                     VIPYNGPNPKTPERTAG"
     gene            complement(2396..3130)
                     /locus_tag="VF276_19000"
     CDS             complement(2396..3130)
                     /locus_tag="VF276_19000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003936171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6608997.1"
                     /translation="MALLQTERLTKVYGKGSAAVTALDHVNLSINPRELVAIMGPSGC
                     GKSTLLHLLGGLDTPTEGRVLLDGVDLSTLNDTALTITRREKIGFVFQFFNLIPVLSA
                     LENVTLPLALGARPKDAPARAAEWLTRLGLGDRLGNRPDQLSGGQQQRVALARALVTE
                     PVLILADEPTGNLDSRAADDMLHLLRQAVDEWGRTVVMVTHDARMAAYADRIIFLKDG
                     TVVDDTRLTTAPAIEDTMQKMVTVRK"
     gene            complement(3168..3809)
                     /locus_tag="VF276_19005"
     CDS             complement(3168..3809)
                     /locus_tag="VF276_19005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX6608998.1"
                     /translation="MTSSLTRSKIERGPVQHALLALLSQQSRHGYELHDLFEAAVGGH
                     WELNSGQIYSSLERLARDGLVYEEGVEKGGGPDKRVWALTDEGRASLLRWWHSAVPRD
                     YRLRDEFYLKLMLAIVTRAERPHLVLHVQRRELFQELHDLTTRRNAANPAQELAQILL
                     LDSAIMHTEAELRWLDMVESRLDDIRAQPLPLPAPRQRGRPRKVSPDKNGAGK"
BASE COUNT          612 a         1365 c         1188 g          666 t
ORIGIN      
        1 tctactgacc actgaccacc gaccactgac cactcgtccc gtttgacgcc cgtggtacaa
       61 atgtgctata cttgccgctt agaagccccc cgcctggggg gccggcggct ggttcacctg
      121 ggaggcccac cgatcgacgc cgcgaatttc cgcgaccgca tgcgcgccat gggcgtgcaa
      181 cccctcaccc gccccgctcc gcgcgatccg gcggagggga tgcccttccc tgcgcccggc
      241 atagacggac tgcccgacga ggcgctcccg gccaccgagg ccgaggcgcg cccgccgctg
      301 gaggaggcgc tgccgggcgc ctggcacgac accccgcatg gccgctgctt cgccatcgaa
      361 taccgctaca cgctcgatca tcagcacggc agcgtgccgc tggcgcagtt gctggacctg
      421 cccggcgaga ccctgagcca cctggggcgc aacggcgatt ttgccggggt cgaccggcgg
      481 cggctgctct tcttcgacac cgagaccacc ggcctggccg gcggcaccgg cacctatgtc
      541 ttcctggccg gcctcggctt cttcgacggc gacagcttcg tcgtacgcca gttgttcctg
      601 cccgacctgc aatccgagcg cgcgctgctg cacgccttca acgacctgct gggccgcagc
      661 gcctgcctgg tcagcttcaa cggacgcgcc ttcgactggc cgctgatcga gtcgcgcttc
      721 acgctcagcc ggatgcgccc ggtgcagggc gcgcccctgc atctcgacct gctggcgcct
      781 gcccgccggg tctggaaaga ccggctgccg tcgtgcgcgc tgggatcgct ggaagcgcac
      841 gcgctccact tccgccgccg gggcgacgta cccggctggc tcatcccctc gctctatttc
      901 cagtacctgc gcggcggcgg catcaacgcg ctgctgcccg tcttcgagca caaccggctc
      961 gacgtgctgt cgctgctggc gctggcgggg tatatgggca gcctgctgca cgcgcccgac
     1021 gccgcgaacc tggagagcgc cgagtgctac ggcctcgcgc gcctgtacga agacctggac
     1081 aactacgagg ccgccgtccg ctgctacgaa cgcgccttga agggcgccat cagcccggcc
     1141 ctgcgcgccc tcaccctgca acggctgccc atggcccaca agaagctaag ccaccaccac
     1201 gaggcgctgc gcatctggga agagctggtg gcccgcgaga ccacgctggt cttcccctac
     1261 atcgagcttg ccaagcacta cgaacaccac acccgcgagt acgcccaagc cgtcgccctg
     1321 gtcaaccacg ccctggccct ctgccaggac ccctgggtgc gggccaccct gccgcgcaac
     1381 accggccccg acctcgaacg ccggcgggtc cgcctcgaaa ccaagatcgc ccgcgccgcc
     1441 accgggccct ctcctaaaaa tcgaggccgt gctgccgagg ggtgcgccga ggctgtcctt
     1501 taggcttcga ccccgccgcc cccgcgcctg ttccgcccgc cccaggaatg ctccgcgcgt
     1561 cgaaccggag tccggcattc tttttgctta ggagccggtt gagggttggc tgcaggattt
     1621 ccgtttgtgg gaacagcgat gaaatgcttc acggcgcccc atggccggcg caatcgcatc
     1681 cccgcacaat tgatgccgca cccaggaagc aaccccgccg ctggtaaatg gaggttcctt
     1741 ctcgactatg gatgcaatct tcgacattga ctggcgccgt ctcttcgttc ctgaattacc
     1801 cattcttgaa atcctcattc ggggcacggt gatctatctg gggatcttca tactcttgcg
     1861 gctggtattg aagcgcgaga ccgggacgct gggtatcacc gatctgctcg tcgtcgtgtt
     1921 cctggcggac gcggcgcaaa acgctatggc cggtgactac cactccatta cagagggcct
     1981 gatcctcgtg ggcacgatcg tcttctggag ttacgccctc agttggcttg gttatcgttt
     2041 cccccgtgtg cagcggttca tcaatccccc accactcctg ctcgtcaaag agggccgcat
     2101 gattccgcgt aacttgcggc aggaactcct gacgtctgac gagctaatga gccagttgcg
     2161 ccagcaagga gtccgggatc tcgccgaagt aaaagaggca tttatggagg gagacggccg
     2221 gatcagcgtg attccctaca acggccctaa tccgaagaca ccggagagaa ctgccgggta
     2281 accgccggcc ttgatccgcg cccggaaggc ggcccggcag ctagcgaacc gccaacatca
     2341 cgcacgaagt caccaggttt gcgccacgct cagggatagc cggcgcggcg ggccgctact
     2401 tgcggaccgt gaccatcttc tgcatcgtat cttcaatggc gggcgcggtc gtcaggcggg
     2461 tgtcgtcgac cacggtgccg tctttgagga agatgatcct gtcggcatag gcggccatgc
     2521 gcgcgtcatg cgtcaccatg accacggtgc ggccccattc gtccacggcc tggcgcaaca
     2581 ggtgcagcat gtcgtcggcg gcgcgcgagt ccaggttgcc ggtcggctca tcggccagga
     2641 tcagcaccgg ttcggtcacg agcgcgcggg ccagggccac gcgctgctgc tggccgccgg
     2701 aaagctggtc gggccggttg cccaaccggt cgcccaggcc caaccgggtc agccactcgg
     2761 cggcgcgcgc cggcgcgtcc ttgggccggg cgcccagggc cagcggcagc gtcacgttct
     2821 ccagcgccga cagcaccggg atcaggttga agaactggaa cacgaagccg atcttctcgc
     2881 gccgggtaat ggtgagcgcc gtatcgttca gcgtcgagag gtccacgccg tcgagcagca
     2941 cgcggccctc ggtcggcgtg tccaggccgc caagcagatg cagcagcgtc gatttgccgc
     3001 agccgctcgg ccccatgatc gccaccagtt cccgcggatt gatgctcagg ttgacgtgat
     3061 ccagcgccgt aaccgccgcg ctgcccttgc catagacctt ggtcagccgt tcggtttgca
     3121 aaagggccat gagaagtctc ctctacctct ccgtactgca tgcgccgtta tttgccggcg
     3181 ccgttcttgt cgggggatac cttgcggggc cgcccgcgct gccggggggc gggcagcggc
     3241 agcggttgcg cgcgaatgtc gtccagccgg ctttccacca tatcgagcca gcgcaactcg
     3301 gcctcggtgt gcatgatcgc gctgtcgagc aacaggatct gcgccagttc ctgggccggg
     3361 ttcgccgcgt tgcgccgcgt ggtcaggtcg tgcaactcct ggaagagttc gcgccgctgc
     3421 acatggagca ccagatgcgg acgctcggcc cgcgtgacaa tcgccagcat cagcttcagg
     3481 taaaactcat cgcgcaggcg gtagtcgcgg ggcaccgccg agtgccacca gcgcagcagg
     3541 ctcgcgcgcc cctcgtcggt cagcgcccag acccgtttgt ccggcccgcc gcccttctcc
     3601 accccttctt catacaccag cccgtcgcgg gccagccgtt cgaggctgct gtagatctgg
     3661 cccgagttca actcccagtg cccgcccacg gcggcctcga agagatcgtg cagttcatac
     3721 ccatggcgcg attgctgcga cagcaacgcc agcagcgcat gttgcacggg gccgcgctcg
     3781 attttgcttc gggtcaggct tgaggtcata aatacctact tgctatctag a
//
LOCUS       DASUYC010001165         2391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_672561,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001165 DASUYC010000000
VERSION     DASUYC010001165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672561"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /gene="hslU"
                     /locus_tag="VF276_19010"
     CDS             <1..1028
                     /gene="hslU"
                     /locus_tag="VF276_19010"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HEX6608999.1"
                     /translation="GAQLEATRQGGGENVPLDDASSGNATIVAAAPDTGAGRSPAAEK
                     ARHRQQQRLARQLAAQQLEEEQVEIEIEGDNTPFSSVLEFVPGMSQDEMAASFQDFLD
                     SFPQGRRRSRRVSVKEARRILTEEEAHKLLDFDSIIETALQRVQESGVIFIDEIDKIT
                     GPAVEMGADVSGEGVQRDLLPIVEGSTVQTRYGSVKSDHILFIAAGAFHQSRPSDLIP
                     ELQGRFPLRVELAGLSQEDLEAILTQPENALTKQYQALLATEGVEIDYTPEALKAIAE
                     MAAILNERTEDIGARRLQTIMEQVLEELSFEADTRNGEKVLIDEEFVNKRMEGFIEDE
                     DLSRFIL"
     gene            complement(1133..1747)
                     /locus_tag="VF276_19015"
     CDS             complement(1133..1747)
                     /locus_tag="VF276_19015"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEX6609000.1"
                     /translation="MESHTGPDLGSIILVGGQSRRMGRNKALLRLTPDGPTLIEQVIA
                     AVAPFGPVLLVTNTPADYAFLNLPMVPDTPLGQGPLAGLYSGLEATTARYNFVLACDM
                     PRLQPALLGHMAALPREYDVLVPRWTAPDGTEQLETLHAIYSKACLPAIGECLQRGRL
                     RMIAFYPAVRVQYLDEPELRRRDPALASFRNLNTPDEVAGLDTP"
     gene            complement(1983..>2391)
                     /locus_tag="VF276_19020"
     CDS             complement(1983..>2391)
                     /locus_tag="VF276_19020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6609001.1"
                     /translation="FLVAALAALFAVGAPAYAAASVCRTDPVVILTDGTIIDISASID
                     TSLFNLQHVTYTVHAPAGSRLLAVVHTPNLPGTTESFQFYDDGPDNQIQTDTIVSTRT
                     SNVGVTATTLVGLRLRSVSGMANEHLVVQLPRR"
BASE COUNT          418 a          727 c          863 g          383 t
ORIGIN      
        1 ggggcgccca actggaggcc acccgccagg gcggcggcga gaacgtcccg ctcgatgacg
       61 cgagcagcgg gaacgcgacc atcgtcgccg ccgcccccga taccggggcc ggccggtcgc
      121 ccgccgccga gaaggcccgc caccgccagc agcagcgcct ggcccgccag ttggccgcgc
      181 agcagttgga agaagaacag gtcgagatcg agatcgaggg cgataatacc ccgttcagca
      241 gcgtgctgga gtttgtgccc ggcatgagcc aggacgagat ggcggccagc ttccaggatt
      301 tcctggacag cttcccgcag ggtcgccgcc gttcgcgccg cgtctcggtt aaggaagcac
      361 gccgcatcct gaccgaggag gaagcgcaca agctgctcga cttcgacagc atcatcgaaa
      421 cggcgctaca gcgagtgcag gagagcgggg tcatcttcat cgacgagatc gacaagatta
      481 ccggccccgc cgtggagatg ggcgccgatg tgtcgggcga gggcgtgcag cgcgacctgc
      541 tgcccattgt cgagggatcg acggtgcaga cgcgctacgg ctcggtcaag agcgatcaca
      601 tcctgttcat cgcggcgggg gcgttccacc agtcgcgccc gtcggacctc attccggagt
      661 tgcagggccg cttcccgctg cgcgtcgaac tggccgggct gagccaggag gacctggagg
      721 cgatcctgac ccagccggag aacgcactga ccaagcagta ccaggcgctg ctggcgaccg
      781 agggcgtgga gatcgactat acgccggagg cgctgaaggc catcgccgag atggcggcca
      841 tcctcaatga gcgtacggaa gacatcggcg cccgccgctt gcagacgatc atggagcagg
      901 tgctggagga actgtccttc gaggccgaca cccgcaacgg cgagaaggta ctgatcgacg
      961 aagagtttgt caacaagcgc atggaaggct ttatcgagga cgaagacctg agccgcttca
     1021 tcctataaat ccgcgcggca atactgtaca tgcaagaggc gtgtcccacc gggacacgcc
     1081 tctttgtata gccttatcgg ctgggtcatt tatccagtcg gtgcctccgg gtctaggggg
     1141 tgtccaaccc ggcgacctcg tcgggggtgt tgaggttgcg gaagctggcg agggcggggt
     1201 cgcggcggcg cagttcgggt tcgtcgaggt actggacgcg cacggcgggg tagaaggcga
     1261 tcatgcgcag acggccccgc tgcaagcact cgccgatggc gggcaggcag gctttgctgt
     1321 agatggcgtg gagcgtctcc agttgttcgg taccgtcggg cgccgtccag cggggcacca
     1381 gcacatcgta ctcgcggggc agggcggcca tgtggcccag cagggccggt tgcaggcggg
     1441 gcatatcgca ggccagcacg aaattgtagc gggcggtggt ggcttccagg ccactgtaca
     1501 gtccggcgag cggtccctgg ccgaggggtg tgtcgggcac catgggcagg ttgaggaagg
     1561 cgtagtcagc cggggtgttg gtcaccagca gcaccggccc gaagggcgcc acggcggcga
     1621 tgacctgttc gatcagcgtc gggccgtccg gggtgagccg cagcagcgcc ttattgcgcc
     1681 ccatgcgccg gctttgccca ccgaccaaaa tgatgctgcc gagatcggga ccggtgtggc
     1741 tctccatatg catccctccg ccgtaattcg cgcatggccc tgctgcgcta ccattatacc
     1801 tgatcggtgg gaacgcggtg ggcgccggct gggggcgttg tgcatggcag ggggttggtg
     1861 ggaatcgatc cggaggccct cgaacgacag cgggcgccgg gatccgtggc ccgcggttcg
     1921 acactgaacc gccggctgga cccaggcacc cgttgtttgc ggatcgggtt tgcccgcgtg
     1981 ggttagcggc gcgggagttg caccaccagg tgctcgttcg ccatgccgct gaccgaccgt
     2041 aaccgcaggc caacgagggt cgtggccgtc acgccgacgt tgctcgtgcg ggtgctcacg
     2101 atcgtatcgg tctggatctg gttgtccggt ccgtcgtcgt aaaactggaa cgactcggtt
     2161 gtgcccggca aattcggcgt gtgaaccacc gccagcagcc gcgacccggc gggcgcgtgc
     2221 acggtatagg tcacgtgctg gaggttaaac aggctggtgt cgatggacgc actgatgtcg
     2281 atgatcgtgc cgtcggtcaa gatgacgacg gggtcggtgc ggcagacgct ggcggcggcg
     2341 taggcggggg cgcccacggc aaacaacgcc gccagtgcgg caaccaggaa c
//
LOCUS       DASUYC010001166         2616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_2058741, whole genome shotgun sequence.
ACCESSION   DASUYC010001166 DASUYC010000000
VERSION     DASUYC010001166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2058741"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VF276_19025"
     CDS             <1..395
                     /locus_tag="VF276_19025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6609002.1"
                     /translation="IAESYERIHRSNLVGMGVLPLQFLNGESSQTLGLTGREVFDIDG
                     LAEVIASNLGMREVTVRARPVEGEEGASAEFHALVRIDTPQEVLYYRHGGILQYVLRQ
                     LLSGHDEPEVVSKGLATVADPATGSVPT"
     gene            complement(474..>2616)
                     /locus_tag="VF276_19030"
     CDS             complement(474..>2616)
                     /locus_tag="VF276_19030"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEX6609003.1"
                     /translation="KAEARKSFALLRMARLDAEAAIAPIPQAARRLTMPRRITHGVSA
                     LMLVLTLVLMNGIAQATPALPAASDAPSAAGDWPMYGHDAARTNYNAAETAIGAANVD
                     QLVPRWEYHIGSGADQTSSAPSVAGGRVYVGSSVTSGDNFFALDAVTGGFAWSADLGY
                     TNDCWHVGIGATPAVAGDLVVAGGGAATYYGLDAATGAQRWSHALNAGGSAFAWASPL
                     LANGRAYIGVASYCDNPSVQGAVQALDLATGNVLANQPIVPSGRAGGGVWNSPALSPD
                     GSTLIAATGEDYAGFNGPYTRAMVSFDPGSLAVREAHQEGSTNQDLDFGTTPIIFHDS
                     QNRTLVGANHKDGTFYAYLLSNINAGPVWSRDTGTTVGFMPAYDPTYGAGGTLFIAGG
                     GARLYAVDPATGADRRADTSSGDSHGNMAIASGLIFLNNGGDLDIRDEHTGNLLRTLN
                     PTHPGGALSGVAVANGFIYWLSGGYLNAWSLPGFVPPTATPPGPTPTPGGCGITFSDV
                     FPTDYYYEPVRYLYCAGILSGYSDNTFRPGAGTTRGQMVKIIANAYHIPAYTPAQPTF
                     SDVPAAHPFYAFIEAAAHAGIVSGYSDGTFRPGAGVTRGQLAKIVVNAAGWPLQTPAQ
                     PVFTDVPAGSPFYEAIATAACHGIISGYADGSFRPGAGATRGQISKIVYLALTNSGGC
                     AAPALAAAAMSGAGGVEFSTARLEHLCIIEH"
BASE COUNT          389 a          818 c         1013 g          396 t
ORIGIN      
        1 tgatcgcgga gagctacgag cgcatccacc gcagcaacct ggtggggatg ggcgtgctgc
       61 cgctgcagtt cctcaacggc gaaagctcgc agacgctggg cctgaccggc cgcgaggtgt
      121 tcgacatcga cgggctggcc gaggtgatcg ccagtaacct gggcatgcgc gaggtgacgg
      181 tgcgcgcccg cccggtggaa ggcgaggagg gggccagcgc cgagttccac gcgctggtgc
      241 gcatcgacac gccgcaggag gtgctgtact accgccacgg cggcatcctg caatatgtgc
      301 tgcgccagct cctgagcggc cacgacgagc cggaagtcgt ctcgaagggc ctggcgacgg
      361 tggccgaccc ggcaacgggc agcgtgccta cctgagccgg ctggtaagcg aatataagca
      421 acgggccggg gagcgtgctc cccggcccgt cgtttgtgcc tgacgcgcgg gcctcaatgc
      481 tcgatgatgc aaaggtgttc caggcgcgcg gtcgaaaact ccaccccgcc cgcgccgctc
      541 atggccgcag cggcgagcgc gggggccgcg cagccgccgc tgttggtcag cgccaggtag
      601 acgatcttgc tgatctggcc gcgggtggcg cccgcgccgg ggcgaaagct gccgtcggcg
      661 tagccgctga tgatgccgtg gcaggccgcc gtggcgatgg cttcatagaa ggggctgccc
      721 gccggcacgt cggtgaagac cggctgagcg ggggtttgca gcggccagcc cgcggcgttg
      781 accacgatct tggcgagttg gccgcgggtg acgcccgcgc cgggacggaa ggtgccgtcg
      841 ctgtagccgg agacaatgcc ggcgtgggcg gcggcctcga tgaaggcgta gaaggggtgg
      901 gcggcgggta cgtcgctgaa ggtgggctgg gcgggcgtat aggccgggat atgataggcg
      961 ttggcgataa tcttgaccat ctggccgcgg gtggtgccgg cgccggggcg gaaggtattg
     1021 tcgctgtagc cgctcaggat accggcgcag tagagatagc gcaccggctc atagtagtag
     1081 tcggtgggga acacgtcgct gaaggtgatg ccgcagccac ccggcgtcgg cgtggggccg
     1141 ggcggggtag cggtgggcgg cacgaaaccg ggcaggctcc aggcgttgag gtagccgccc
     1201 gatagccagt agatgaaacc gttggcgacg gccacgcccg acagggcgcc gccggggtgc
     1261 gtgggattca gcgtgcgcaa caaattgccg gtatgctcgt cgcggatgtc caggtcgccg
     1321 ccgttgttga ggaagatcag gccgctggca atcgccatgt tgccgtgtga gtcgcccgac
     1381 gaggtgtcgg cccgccggtc ggcgccggtt gccgggtcga cggcgtagag gcgggcaccg
     1441 ccgccggcga tgaacagcgt gcccccggcg ccgtacgtgg gatcgtaggc cggcatgaag
     1501 ccgacggtcg tgccggtgtc gcggctccag accggcccgg cgttgatgtt gctcagcagg
     1561 taggcgtaga aggtgccgtc cttgtggttg gcccccacca gggtgcggtt ctggctgtcg
     1621 tggaagatga tcggcgtggt gccgaagtcg agatcctggt tggtcgagcc ttcctggtgc
     1681 gcctcgcgca cggccaggct gccggggtcg aacgagacca tggcgcgggt gtaggggccg
     1741 ttgaagcccg cgtagtcctc gccggtggcg gcgatcagcg tgctgccgtc gggactcagc
     1801 gctggggagt tccagacgcc accgcccgcc cgcccgctgg ggacgatggg ctggttcgcc
     1861 agcacgttgc cggtagcgag gtcgagcgcc tgaacagcac cctggaccga gggattatcg
     1921 cagtaggacg ccaccccgat gtaggcgcgg ccattggcga gcaagggcga ggcccaggcg
     1981 aaggcgctgc cgcccgcgtt gagggcatgg ctccagcgtt gcgcgcccgt cgcggcatcc
     2041 agcccataat aggtggcggc gccgccgccg gccacgacga gatcgcccgc gacggcgggc
     2101 gtcgcgccga tgcccacgtg ccagcaatcg ttggtatagc ccaggtcggc gctccaggcg
     2161 aaccccccgg tcacggcgtc gagcgcgaag aagttgtcgc cggaggtcac gctgctgccc
     2221 acatacacgc gcccgcccgc gacgctgggc gcgctcgatg tctggtcggc gccgctgccg
     2281 atatggtact cccagcgcgg caccagttgg tcgacgttgg cggcgccgat ggccgtctcc
     2341 gccgcgttgt aattcgtgcg cgcggcgtcg tggccgtaca tcggccaatc gcccgcggca
     2401 gatggggcgt cggatgccgc gggcagagca ggggtcgcct gagcgatgcc gttcatcagc
     2461 accagcgtga gcacgagcat gagggcggag acgccgtgcg tgatccggcg tggcatggtg
     2521 agcctccttg ccgcctgcgg gatcggcgcg atagccgcct cggcgtcgag gcgcgccatc
     2581 ctgagcaggg caaatgactt gcgggcctcc gccttg
//
LOCUS       DASUYC010001167         7936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1827913, whole genome shotgun sequence.
ACCESSION   DASUYC010001167 DASUYC010000000
VERSION     DASUYC010001167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 7936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7936
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1827913"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1482
                     /locus_tag="VF276_19035"
     CDS             <1..1482
                     /locus_tag="VF276_19035"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6609004.1"
                     /translation="YRLRSAAAVPLIYQGQAVGVLSLYARRPAAFEAIDMALLVVMAD
                     HAAVALQNAVHYQEVNQARARSQALLKVTQAINQSVDLDTVIELIVQSAVELLRAAGA
                     AVYLLKRAVPVHDPTEGGWITAHSTVGLEESFTQQIGELPMEQSVAGRAILTRETQIV
                     DDTLAVSDIVFPRLSSGDALRSLVVVPLRVGDDMLGVLSTYAARSHAFNPTAIQLLEA
                     FGAQASTAIYNARLYQEAQRGREAAESEQQRLRELEQMKDEFLSTAAHELRTPLTTIR
                     MSAGLAYEQLRMLTAAPDGGEAAWLDPRLANLVGLVLESSERMHSLVNDLLDLTRLEQ
                     GRTQLIIEDLDMREVINASLEVTRPLFGSKNQQISFHMPEARYMVRGDRPHLEQVLIN
                     LLSNAHKYSPEGSQVDVRLTRSGGECLVMVRDQGPGVPEDERERIFERFYRSSLHRQD
                     RTPSTGLGLPIARTITEMHRGRLVVEPAPGGGSIFTLALPIAA"
     gene            complement(1534..1761)
                     /locus_tag="VF276_19040"
     CDS             complement(1534..1761)
                     /locus_tag="VF276_19040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6609005.1"
                     /translation="MNQQQTVIPASQISPVRMLRIEGAERDPYVFGVKDIRRLDRSED
                     GQPIGVASAATVRAADPLRSLHIYELVNCTV"
     gene            2135..4408
                     /locus_tag="VF276_19045"
     CDS             2135..4408
                     /locus_tag="VF276_19045"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0050840 - extracellular matrix binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APA family fibronectin-binding glycoprotein"
                     /protein_id="HEX6609006.1"
                     /translation="MDGSQDGSLAARVDQLEQRVQDLQQHTRQLETALAAAGIVVLPL
                     PDVLPERGPHPPRPSAAPTQAMPGVPRQPGAPATTPIGAPPVIPTPPPGVRSTADLHT
                     GALPNAPLPVRRVRPASPSGGFDLVRNGEFWLNKVGISLLLFGVAFLFKYSVDQGWLV
                     PSVRVGIGGAIGLILLVLGLRLYEDRPHFSQVLLGGSIGAFYISVYAGYQLFGLMPYN
                     VAFGLMVSVTVLAFLLAVRQNAAVLSLIGTAGGLATPFVLYSAAHNLPGLIAYVGLVL
                     LGTSAIYFYRGWHSLIWTAFAGVWATFAVAELDFLWLGAHRPADRWALQLGFAAGWLA
                     FAALPVIRAVLGTRNPEGGQHPAFDPPAAAVPGAPARRPDRHVYLLTLITPVLALGLS
                     VPIWDFSRTTWGLLILAGSAGYILAARALREVHPALGYTHALLALSFATLALVLLLEG
                     SALYFALAAEATMLHLLAHRLKDRGTALAGHVLFAVVGIWFLWRVLFTYMPAARPFDA
                     AALTDLVVIGLALLVSAVVDGPDAVWTYRVLGYLAGLAWLWRQWGGPGAVGDYLSAGW
                     AIYSAGLLYLAIRSGTQYINGRPTPEWAAGHIGFLALGVWFVIRLPLAGLPDSALANA
                     RALSDLAAIGLVLGVATWLLRRDLAWLYRLAAHLALLAWFWHELSGLPNGNGLVTVTW
                     TVYALALLIAGLPSTERRLIYGALGTLLLVVAKLFLIDLVALEAIWRILLFLSIGSMF
                     LVLSYYLQSWLRRTQGV"
     gene            4666..6843
                     /locus_tag="VF276_19050"
     CDS             4666..6843
                     /locus_tag="VF276_19050"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX6609007.1"
                     /translation="MKAFTDWLGRRWARSLSVKLEVLLIVSTLGPVGVLLLLLWLTGS
                     LWSALLGLVVMQGVVLILGRRAIIWLIRPLRELTTRLRLTAAGDLRHRLPTNTATAQA
                     AALQGFVAQRVDAILLAAVDDQSLVPAAQAAHRAGIPVIAVDSRLAEAQATNAVVMTD
                     NHGGGRMAGEALIRLLDGQGQVAVVGLSPHQSHGRAREAGFREALAAAPGVDIVTVQY
                     AFGDKALGRRLTEDILRTYPALAAIYAVNEGAACGVLMALRGHAPRERRVRVVAWDAA
                     PEELQALESGLLDALIVQNAWMLGYCAIDTALAVIEGRAGPPVVHLATRLIRQADLAS
                     PEVRAWLSGAPPTGHTPPPRAPVQLYRIGFARKGRGAVFWEQVTAGAVAAARDCGVRL
                     LYHDSAEGDADELGALAETFNQRIDSIRSLVLRLQQEAGVIGPRAQELVQSAAGQAAL
                     AREQTAALERLSHGVDRMSLAAQQIVAGTEAVAESAAGTLRGVTQAEVAVNDSSQRLQ
                     AIVERLTESLNLLSGRSEQVTSGADAMSDIADETHLLSLNATIEAAGAGAYGQRFAVV
                     AEAVRELAGQALNTTQEFQSIAEEMRVAARAALAATQDSERGTDLSMDLALQARDAIQ
                     GIAGLAQHTSDAVQAITRAVAEQQDTNTELAGFAGQVTATAREAATASAALSQVAQDL
                     TAVVARLQESVAAFHIAETGSPGAQPAGGPERRSAPNPSVARV"
     gene            6959..>7936
                     /locus_tag="VF276_19055"
     CDS             6959..>7936
                     /locus_tag="VF276_19055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HEX6609008.1"
                     /translation="MPTLDAPIVAVTVFTDRARVTRRGTIHLPPGEHALALTGLPLGL
                     DEDSVRAAGRGAQVRILGVEVRTDVVTQAPDPNQAALEARLQALQDQDKALEDAEATQ
                     AARTRFLDTLTARGAAGLAKTFAAGTATLDNVETLAAYLTREHEAVAARCRELAAQRR
                     ALAAEIAAVRARIAPPVPPANSARRQRTIVVAVDAAAETDLELEVLYAVRDASWTPLY
                     DIRLVDNRVTLTYLAHVRQHTGEDWPAVPLSLSTARPAVRATIPRLDAWYIEAFRVPP
                     PVAAPRAYGSAAGGTGPLPALPPAAAEPQYMAAPPPPPPPVQQATAVIES"
BASE COUNT         1141 a         2803 c         2697 g         1295 t
ORIGIN      
        1 tatcggttgc gctcggcggc cgccgttccg ctcatctacc agggccaggc cgtgggcgtc
       61 ctgagtctct acgcccgccg ccccgccgcg ttcgaggcca tcgacatggc gctgctggtg
      121 gtcatggccg accatgccgc cgtggccctg caaaacgccg tccactacca ggaggtgaac
      181 caggcgcggg cgcgctcgca ggcgctgctc aaagtgaccc aggccatcaa ccagtcggtc
      241 gaccttgaca ccgtgatcga gttgattgtg caatctgcgg tggaactgtt gcgggccgcg
      301 ggcgcggccg tctacttgct gaagcgggct gtccccgtcc acgacccgac ggagggcggg
      361 tggatcaccg cgcactccac ggtggggctg gaagagtcgt ttacccagca gatcggcgaa
      421 ttgccgatgg agcagagcgt ggccgggcgg gccattctga cccgcgaaac gcagatcgtg
      481 gacgacactc tggcggtgtc cgatatcgtc ttcccgcgcc tgtccagcgg cgatgcgcta
      541 cgctcgttgg tagtggtgcc gttgcgggtg ggggacgaca tgctgggcgt gttgagcacc
      601 tacgccgcgc gctcccacgc gtttaacccg accgccatcc agttgctgga ggcgtttggc
      661 gcgcaggcca gcacggccat ctacaacgcg cgcctctacc aggaggccca gcgcgggcgc
      721 gaggccgccg aatccgagca gcagcggttg cgcgaactgg agcagatgaa ggacgagttc
      781 ctctccaccg ccgcccacga gttgcgcacg ccgctgacga cgatccgcat gtcggcgggc
      841 ctggcctacg agcagttgcg tatgctgacc gcggcgccgg acgggggcga ggccgcctgg
      901 ctcgacccgc ggctcgccaa cctggtcggc ctggtgctgg aaagcagcga acggatgcat
      961 tcgctggtga acgatctgct cgatctgacc cggctcgaac aggggcgcac ccagcttatc
     1021 attgaagacc tcgacatgcg cgaggtgatc aacgcctcgc tggaagtgac ccggccgctc
     1081 ttcggcagca agaaccagca gatcagcttc cacatgcccg aggcccgcta catggtgcgc
     1141 ggcgaccgcc cgcacctgga gcaggtactg atcaacttgc tgtcgaacgc gcacaagtat
     1201 tcgccggaag gcagccaggt ggatgtgcgg ctgacgcgat ccggcggcga gtgcctggtc
     1261 atggtccgcg accaggggcc cggcgtgccg gaggacgagc gcgagcggat cttcgagcgc
     1321 ttctatcgga gcagcctgca ccgccaggat cgcacgccca gcaccggcct cggcctgccc
     1381 atcgcgcgca ccatcaccga gatgcaccgg ggccgcctcg tggtcgaacc ggcgccgggc
     1441 ggcggcagca tcttcacgct ggccctgccg attgccgcgt gaggcgggcg gccctgctgc
     1501 ccgcgctgtg atccctggcc gccggctcct ccgctatacg gtgcagttga ccagttcgta
     1561 gatgtgcaag ctgcgcaacg gatcggcggc gcgcaccgtg gccgcacttg ccaccccgat
     1621 cggttggccg tcctctgagc gatccagccg gcggatatcc ttcaccccga acacataagg
     1681 atcgcgttcc gcgccctcga ttcgcaacat ccgcacgggt gagatctggg atgcgggaat
     1741 aactgtctgc tgctggttca tctgttggtc ctcccgtggt ggtgtgccgg tggcggatgg
     1801 gcgcggggaa gagtcgaatc gagcccccgt aaacggcctc tgtaccttat ctgtacctta
     1861 tctgtacctt atctgtaata taacaaacgc gtctggacaa agcggtaagc gttggtaaac
     1921 gaacggtgaa aatccgagta acatgtcccg gcacggcgcc ctctacccac caccggctcg
     1981 gcaagactac ctagtgccct cggtcctacg gcatcacaaa atagagcaga tataccgatg
     2041 cggccccttc cggctctcgg ctatcattaa gctggacccc ttcacatctt cctgacgcaa
     2101 ctggtatagg tatatcgaga aagaggtgga caggatggat ggatcgcaag acggcagcct
     2161 ggccgcgcgc gtggatcagt tggagcagcg tgtgcaggat ctgcagcagc atactcgcca
     2221 actagagact gcgctggcag cggcggggat cgtggtcctg ccgctgccag atgtgctacc
     2281 ggagcgcggg ccgcacccgc cgcggcccag cgcggcgccg acgcaggcca tgccgggcgt
     2341 gccgcggcag cccggcgccc ccgcaaccac gccgataggc gcgccgccgg tgatcccgac
     2401 tccgccgcca ggggtccgct cgaccgccga tctacacacc ggtgcgctcc ctaacgcgcc
     2461 actgccggtg cggcgcgtcc ggcccgcctc gccgtccggc ggcttcgacc tggtgcgcaa
     2521 cggcgaattc tggctgaaca aggtgggcat cagcctgctg ctatttggcg tggccttctt
     2581 gttcaagtac tcggtagacc agggatggct ggtgccgtcc gtgcgcgtgg gcatcggcgg
     2641 ggcgattggt ctgattttgc tggtgctggg gctgcgcctg tacgaggatc gcccgcactt
     2701 cagccaggtg ctcctgggcg gcagtatcgg cgcgttctac atctcggtgt atgccggcta
     2761 ccagctcttt ggcctgatgc cctataacgt ggccttcggg ttgatggtca gcgttaccgt
     2821 gctggccttc ctgctggccg tgcgccagaa tgcggccgtg ctctcgctca tcggcacggc
     2881 aggcggatta gcgacgcctt ttgtgctcta cagcgccgcg cacaatctgc cgggcctgat
     2941 cgcctatgtc ggcctggtcc tactggggac gagcgccatc tacttctacc ggggctggca
     3001 ttcgctcatc tggaccgcgt tcgccggggt gtgggcgacc tttgccgtgg cggaactcga
     3061 cttcctgtgg ttgggggcgc atcggcctgc cgaccgctgg gccttgcaac tgggctttgc
     3121 cgccggatgg ctggctttcg ccgcgctgcc cgtgatccgc gctgtcctcg ggacgcgtaa
     3181 cccggagggc gggcagcacc cggccttcga tccgccggcg gcagcggtgc ccggcgcccc
     3241 ggcccggcgc cccgatcgcc atgtctacct gctcacgctg atcaccccgg tcctcgccct
     3301 ggggctatcg gtccctatct gggatttctc caggacaacg tggggcctgc tgatcctggc
     3361 cggctcggcc ggctatatcc tcgccgcgcg ggcgctgcgc gaggtccatc cggcgcttgg
     3421 ttatacccac gccctgctgg cattgagctt tgccacgctg gccctggtcc tgttgctgga
     3481 gggcagcgcc ctctatttcg cgctcgccgc ggaagccacc atgctgcatc tgctggccca
     3541 ccgcctgaag gatcgcggca cggcgctggc cgggcacgtg ctctttgcgg tggtcggaat
     3601 ctggttcctc tggcgcgtgt tattcacgta tatgccggcc gcccggcctt tcgatgccgc
     3661 cgcgttgacc gacctggtgg tgattgggct cgccctgctg gtctccgccg tcgtggacgg
     3721 gcccgatgcc gtctggactt accgggtcct cggctatctg gccgggttgg cctggctctg
     3781 gcgtcaatgg ggcggccccg gcgcggtggg cgactacctc agcgccggct gggccatcta
     3841 ctccgccggg ctcctgtact tggcgatccg cagcggcaca cagtacataa acgggcgccc
     3901 gacgccggaa tgggcggcgg ggcacatcgg gttcctggcc ctgggcgtct ggtttgtgat
     3961 ccggctgccg ctggccgggc tgcccgactc ggcgctcgcc aacgcgcggg cactgagcga
     4021 cctggcagcc attggtctgg tgctcggcgt ggccacgtgg ctgctccggc gcgacctggc
     4081 ctggctctac cggctcgcgg cacatctggc gctactggcc tggttctggc acgaactgag
     4141 tggcctgccg aacggcaatg gtctggtgac cgtcacctgg accgtttacg cgctggcatt
     4201 gctgatcgcc ggattgccat ctaccgagcg ccgcctcatc tacggcgcgc tcggtaccct
     4261 gttgctggta gtggcgaaac tcttcctgat cgatctcgtg gccctggaag cgatctggcg
     4321 catcctgctg ttcctcagca tcgggagcat gttcctcgtc ctgagctact acctgcaatc
     4381 ctggctgcgc cggacgcaag gcgtctgacg cgccgtcctt ttcctgcccg ccgcgggcag
     4441 ggggccgggc ggttgggcat atcgctcgcc ccttgcccgc cgcggcgtaa ggtagtcagg
     4501 cgtcaggcgc agtgcggcgc cgttgacagg gctgccgggc ctgtgctaga atccgtttca
     4561 atctgcgatt ggcgccgagg ccgcgccgca accctggggg ttgctatgca ttagcggagg
     4621 gacccggctc atgttactcc tggccgccgg ttccattagc acgccatgaa agcgttcacc
     4681 gactggctgg gccggcgctg ggcgcggtcg ctgagcgtca agctggaagt gctgctgatc
     4741 gtcagcacgc tcggcccggt gggcgtgctg ctgctgctgc tctggctcac cggcagcctc
     4801 tggtcggccc tgctcggcct ggtcgtgatg cagggcgtcg tgctcatcct cgggcgccgg
     4861 gccatcatct ggttgatccg ccccctgcgc gagttgacta cccggctgcg actcacggcc
     4921 gcgggcgatc tccgccaccg tctgcccacc aataccgcga ccgcgcaagc cgccgccctc
     4981 caggggtttg tcgcgcagcg cgtggacgcg atcctgttgg ccgcggttga tgaccagagc
     5041 ctggtccccg cggcgcaggc ggcccaccgg gccggcatcc cggtcatcgc cgtggactcg
     5101 cgcctggccg aggcgcaagc gaccaatgcc gtggtcatga ccgacaacca cggcggcggg
     5161 cgcatggccg gagaggcgct catccgcctg ctggacggcc agggccaggt cgccgtcgtc
     5221 ggcctgtcgc cgcaccaatc gcatgggcgg gcgcgcgagg ccgggttccg cgaagcgctg
     5281 gccgccgcgc ccggcgtgga catcgtcacc gtgcaatatg cctttggcga taaagcgttg
     5341 ggccggcgcc tgaccgaaga catcctgcgc acctacccgg cgctggcggc catttatgcc
     5401 gtcaacgagg gcgcggcctg cggcgtgttg atggcgctgc gcggccatgc gccccgcgag
     5461 cgtcgggtgc gcgtggtggc ctgggatgca gcgcctgaag agttacaggc gctggaatcc
     5521 ggcttgctgg acgcgctgat cgtccagaac gcctggatgc tgggctactg cgccatcgat
     5581 acggctctgg ccgtgatcga ggggcgggcc ggaccgccgg tggtgcatct cgccacgcgc
     5641 ctgatccggc aggcggacct ggcgagcccc gaggtccgcg cttggctcag cggcgcgccc
     5701 cccaccggcc acaccccgcc gccgcgcgcg ccggtacagc tttaccgcat cggcttcgcc
     5761 cgcaagggtc gcggcgccgt cttctgggag caggtcacgg ccggtgccgt agcggcggcg
     5821 cgcgattgcg gcgtgcggct gctgtaccat gacagcgccg aaggcgacgc cgacgaactg
     5881 ggcgcgctcg ccgagacctt caaccagagg atcgacagca ttcgctcgct ggtgctgcgg
     5941 ctgcagcagg aagcgggggt gatcgggccg cgggctcagg aactagtcca gtcggcggcg
     6001 ggccaggccg cgctggcccg cgaacagacg gcggccctgg agcgcctgag ccacggcgtc
     6061 gaccgcatga gcctggccgc ccagcagatc gtggccggca ccgaggcggt ggccgagagc
     6121 gccgccggca ccctgcgcgg tgtcacccag gccgaagtcg cggtcaacga cagctcgcag
     6181 cgcttgcagg ccatcgtcga acgcctcacc gaatcgttga acctgctcag cgggcgcagc
     6241 gagcaggtca ccagcggcgc cgacgccatg agcgacatcg ccgacgagac gcatctgcta
     6301 tccctgaacg ctaccatcga ggccgccggg gccggggcct acggccagcg cttcgccgtc
     6361 gtcgccgagg ccgtgcgcga actggccggc caggcgctca acaccaccca ggagttccag
     6421 agcatcgccg aggagatgcg cgtcgccgcc cgcgccgcgc tcgccgccac ccaggacagc
     6481 gagcgcggca ccgacctgag catggacctg gcgttgcagg cgcgcgatgc gatccagggc
     6541 atcgccgggc tggcccagca cactagcgac gctgtccagg ccatcacgcg cgccgtggcc
     6601 gagcagcagg acaccaacac cgaactggcg gggtttgccg ggcaggtcac ggccacggcc
     6661 cgcgaggccg ccaccgccag cgccgcgctc agccaggtgg cgcaagacct gacggcggtg
     6721 gtcgcccggt tgcaggagag tgtggccgcc ttccacatcg ccgaaaccgg gtcgccgggc
     6781 gcacaaccgg ccggtggccc cgagcggaga tccgcgccca atccatccgt cgcgcgggtg
     6841 tagcgcccgg ccccgtaacg cgcggcgcgc ccgcgccgta taacaccgtg agtccgggcc
     6901 acaccgccgg actatccagc ctatccaagc actaactacc aatcgaaggg gatacgccat
     6961 gccgactctg gacgccccaa ttgtggcggt caccgtcttt accgatcgcg cccgcgtgac
     7021 ccggcgcggt acgatccacc tgccgcccgg cgagcacgcc ctggcgctga ccggcctgcc
     7081 cctgggcctg gacgaagact cggttcgtgc cgcgggccgc ggggcacagg tccgcatcct
     7141 gggcgtggag gtccgcaccg acgtggtgac ccaggcgccg gatcccaacc aggcggcgct
     7201 ggaggcgcgc ttgcaggcgt tgcaggatca agacaaagcc ctggaggacg ccgaagcgac
     7261 gcaggcggcc cgcactcgct tcctggacac cctgacggcg cgcggggccg ccggcctggc
     7321 caagacattc gccgcgggca ccgccactct cgacaacgtg gagaccctgg ccgcctacct
     7381 gacccgcgag cacgaagccg tcgccgcccg ctgccgcgaa ctggccgccc agcggcgcgc
     7441 gctggccgcc gagatcgccg ccgtgcgggc caggatcgcc ccgccggtgc cgcccgccaa
     7501 ctcggctcgc cgccagcgca ccatcgtcgt cgccgtggac gccgccgccg agaccgacct
     7561 ggaactcgaa gtgctctatg ccgtgcgcga tgcctcgtgg acgccgctct acgatatccg
     7621 gctggtcgac aaccgcgtga cgctgaccta cctggcccac gtgcgccagc acaccggcga
     7681 ggactggccc gccgtgcccc tgtcgctctc gacggctcgg cccgccgtgc gcgccaccat
     7741 ccccaggctc gacgcctggt acatcgaggc ctttcgcgtg ccgccgcccg tcgccgcgcc
     7801 cagagcctac ggcagcgcgg cgggggggac ggggccgctg cctgcgttgc cgcctgccgc
     7861 ggcggaaccg cagtacatgg ccgcgccgcc cccgccgccc ccgcctgtgc aacaggccac
     7921 cgcggtgatc gagagc
//
LOCUS       DASUYC010001168         2428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1365551, whole genome shotgun sequence.
ACCESSION   DASUYC010001168 DASUYC010000000
VERSION     DASUYC010001168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1365551"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..1152
                     /locus_tag="VF276_19060"
     CDS             151..1152
                     /locus_tag="VF276_19060"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6609009.1"
                     /translation="MGQDHAGGGRLAVVAPVVAVAGTTAALAGLNRLIDLQAGPLPEQ
                     LPADPQEYESRFGRVIYYTAGAEDAPPLLLVHGHNAAASAYEFRRQFTRLAQHYHVVA
                     PDLIGYGLSDRPPIEYTAETYIELLGDLLREVVRAPAIVVASSVSGAHAIQVAADDPA
                     WITHLVLVCPTGLSRQTGPDGVGRAVTALLRAPVVGETLFHGLASRAGIRGFLRKQTY
                     ADPACVDDDLVEMNYLSAHQPGARYAPASFVGGALGHDVHDAWPRVGQPALLVWGSEA
                     QITPVGDAAGFLALNPGTELETIESAGLVPHDEQPEEFARIVEHWLERTKDLIPGPV"
     gene            complement(1175..2083)
                     /gene="ftcD"
                     /locus_tag="VF276_19065"
     CDS             complement(1175..2083)
                     /gene="ftcD"
                     /locus_tag="VF276_19065"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HEX6609010.1"
                     /translation="MQKLVECVPNFSEGRRPDRIRRIVGAIENLPGIRLLDVESNPDH
                     NRSVVTFVGEPQATGEAAFRLTQAAVAVLNMEEHEGAHPRIGAMDVIPFVPITGVSMD
                     ECVALARQVGARIGAELDVPVYLYAHAATTPARRRLPDVRQGQYAGLKTAISTPERRP
                     DFGPARMHPTAGATAVGARPPLIAFNINLATRNIHAAREIARAVRESSGGLVNVQAMG
                     VDLAEQGMVQVSMNLLDYTRTPIHRAFELVRLEAERHGATIAGSEIVGLVPVDALLDA
                     ATFYLRLDGFRRDQTLELRLLEAEQQ"
     gene            complement(2162..>2428)
                     /locus_tag="VF276_19070"
     CDS             complement(2162..>2428)
                     /locus_tag="VF276_19070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6609011.1"
                     /translation="TPIDRIPLLLALLAFCAFLYRRLSAQPGFALATLAVLLGTTAGV
                     FALWFLAFAAFGGNVWIGILGVIVECSLGIALGVLGINRLDPAA"
BASE COUNT          399 a          817 c          848 g          364 t
ORIGIN      
        1 atactgcgtg ctactgtcaa actcgcgtgc gcaccgggac gctcacaaga aagctaaaag
       61 acgatcacta gatcgtacta acttagcact cagcaatgtg ctacgcaccg ctacgcgaac
      121 agcactcagc acttaggaag aggtggctat atgggacaag accatgcggg cgggggccgc
      181 ctggcggtag tggcgccggt ggtggccgtg gccgggacaa cggcggcgct ggccgggttg
      241 aaccgcctga tcgacctcca ggccgggccg ttgcccgaac aattgcccgc cgatccccag
      301 gaatacgagt cgcgcttcgg gcgcgtcatc tactacacgg cgggcgccga ggatgcgccg
      361 cccttgctgc tggtccacgg ccacaacgcg gcggcctcgg cctatgagtt tcgccggcag
      421 tttacccgcc tggcgcagca ctaccacgtg gttgcacccg atctgatcgg ctatggcctc
      481 tccgaccggc cccccatcga atacacggcg gagacctaca tcgaactact gggcgatctg
      541 ctgcgcgagg tggtgcgggc gccggccatc gtcgtcgcca gcagcgtgag cggcgcccac
      601 gccatccagg tcgcggcgga cgacccggcg tggatcaccc acctggtgct ggtctgtccg
      661 accgggctga gccggcagac ggggccggac ggggtgggtc gcgccgtcac cgcgctgctg
      721 cgcgcgcccg tggtgggcga gacgctgttc cacgggttgg cgtcgcgggc gggtattcgc
      781 ggtttcctgc gcaagcagac ctacgccgac ccggcctgcg tggacgacga cctggtggag
      841 atgaactacc tgagcgcgca ccagccgggg gcgcgctatg ccccggcctc gtttgtcggc
      901 ggggcgctgg gccacgacgt gcacgacgcc tggccgcgcg tgggccagcc ggcactgctg
      961 gtctggggca gcgaggcgca gatcaccccg gtgggcgacg cagcgggctt cctggccctg
     1021 aaccccggca ccgaactgga gacgatcgag tccgccggtc tcgtgcccca tgacgagcag
     1081 cccgaagaat tcgcccgcat tgttgagcac tggttggagc gcaccaaaga cctgatcccc
     1141 ggcccggtgt gatgcggtgc cgggcgacgg gccgctactg ctgctcggct tcgagcaggc
     1201 gtaattccag cgtctggtcg cggcggaaac cgtcgagccg caggtagaac gtggcggcgt
     1261 cgagcagggc atccaccggc accaggccga cgatctcgct gcccgcgatg gttgccccgt
     1321 gccgctcggc ttccaggcgc accagttcaa aggcgcgatg gatcggagtg cgcgtgtagt
     1381 cgagcaggtt catcgacacc tgcaccatgc cttgctcggc caggtccacg cccatcgcct
     1441 gcacgttgac cagcccgccg ctgctttcgc gcactgcgcg ggcgatctcg cgggcggcgt
     1501 ggatgttgcg cgtcgccagg ttgatgttga aggcgatcag cggcgggcgg gcgcccacgg
     1561 cggtggctcc ggcggtgggg tgcatgcgcg ccgggccgaa gtcggggcgg cgctccggcg
     1621 tgctgatcgc cgtcttcagc ccggcatact ggccctggcg cacatcgggc agccggcggc
     1681 gggccggggt ggtcgccgcg tgcgcgtaga ggtagaccgg cacatccagc tccgcgccga
     1741 tgcgcgcgcc cacctgccgg gccagcgcca cgcactcgtc catgctgacc ccggtgatcg
     1801 gcacgaaggg gatcacgtcc atggcgccga tgcgcgggtg cgcgccttca tgttcctcca
     1861 tgttcagcac ggccaccgcc gcctgggtca gccggaacgc cgcctccccc gtggcctgtg
     1921 gctcgcccac gaacgtcacc accgagcggt tgtgatcggg gttggactcg acatccagca
     1981 gccggatgcc ggggaggttc tcgatcgcgc cgacgatgcg gcggatgcgg tcggggcgcc
     2041 ggccttcgct aaagttcggg acacattcca ccagtttctg catagccgtt tctcccataa
     2101 acacaatagc gcgtagcggg ccgagaagcg gtaccgctac gccgagataa tgagaatgtg
     2161 cctacgcggc agggtccagg cgattgatac ccagcacgcc cagagcaatg cccaggctgc
     2221 actcgacaat gacgcccagg ataccgatcc agacattgcc gccaaaggcc gcgaaagcca
     2281 ggaaccagag cgcgaagacg cccgccgtcg tcccgagcaa gacggccagc gtagccagcg
     2341 cgaagccggg ttgtgccgag agccggcgat agaggaaggc gcagaaggcc agcagcgcca
     2401 gcagcagcgg gatgcggtcg atgggggt
//
LOCUS       DASUYC010001169         5379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_907870,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001169 DASUYC010000000
VERSION     DASUYC010001169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5379
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907870"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..636
                     /locus_tag="VF276_19075"
     CDS             106..636
                     /locus_tag="VF276_19075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011650109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX6609012.1"
                     /translation="MAKLVFGMNQSLDGYVDHLAFAPSPTLFRHFIAEAQGQAGSVYG
                     RRLYEVMRYWDDDHPEWDAEEQAFAAAWRHQPKWVVSRSLPAVGPNARLVADDLAGAI
                     RALKAERDGEIEVGGPALAHSLTELGLIDEYRIYLHPVVLGHGKPYFAGPRPPLRLMA
                     HERIAEDVIRLTYVPA"
     gene            complement(786..1154)
                     /locus_tag="VF276_19080"
     CDS             complement(786..1154)
                     /locus_tag="VF276_19080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEX6609013.1"
                     /translation="MTKHRIYTTSVASVYPHYVAKAEKKGRTQAEVDTIIRWLTGYSA
                     EALAAQLANQIDFEHFFAQAPHLNPARALIKGVVCGVRLEEIAEPTMREIRYLDKLID
                     ELAKGRALEKILRTDPSAGC"
     gene            1296..1517
                     /locus_tag="VF276_19085"
     CDS             1296..1517
                     /locus_tag="VF276_19085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6609014.1"
                     /translation="MSDSRNRLRRLDPPHRCIVQQFSPAVHMRSARAAAARSGLRCLE
                     HLDELERAPALIEVIHSPARTSLRYRVVE"
     gene            2043..2261
                     /locus_tag="VF276_19090"
     CDS             2043..2261
                     /locus_tag="VF276_19090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6609015.1"
                     /translation="MDASLQPGDLVIWWKRVPGSPYVVPLAATVLAVTAHRVKIQADE
                     DGRPVIRYVPPARLQPRDPPNRRERKDR"
     gene            2530..4695
                     /locus_tag="VF276_19095"
     CDS             2530..4695
                     /locus_tag="VF276_19095"
                     /inference="COORDINATES: protein motif:HMM:NF014312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX6609016.1"
                     /translation="MEKRSANRLRYLVAGLVVFLLVAGGLAAWALTQSQVAQANEQAA
                     RASLAHADALRLDADVDSLISSHGPNDLAALLIVRSLNFDYTPQADVQLSAATNWTFP
                     AREFVGHTNGVNDVAISPDGKYLATASTDATAALWNLATGKQLHVFSGYGGSVVTARF
                     SPDGKYLLTSSEDKSVHIWDIATGQSVRVFAGNPDIVDAAVFSPDGRRVAASVEDGIA
                     RVWDIASGQLVLQIRNPGYIGVGYSPDGKYLATGGVDPSHIARIWDASSGQLVQTFTG
                     HTDNVNNPVFSPDGKYLVTPSDDGTVRIWDSATGKTLHVLMGHTGGATGVAFSPGGKR
                     LATGSADRIARIWDVSTGQLVQTLSGHKDGVGGVAFAPDGKWLATASADHTARLWPVQ
                     AATGPRIFAGHTDVVQRATFSPDGKYIATASVDNTARLWDAATGAQLHQFTGHTGWVD
                     TAILSPDGKTLLTSSKDATARLWDVATGTQLRIFRGHTDGLNSAYFSRDGKTIVTASD
                     DKTARLWDVATGKQLRIFRGHTDVVNKAVLVPDAKLVLTGSADGTVRLWDASTGKEVR
                     RYTGHVGPVRPSVSPSGKMMVTASDDGTVRLWDVQSGQELRRFGVPGVAMHTAEFSPD
                     GRMIAAGGRDEEVQVWDVESGQELRRFNGQGGEVRSVAFSGDGKQILVANADKTARLW
                     PADYHDTIRALCGELTRDLTPDERTQYGITDQGPTCLAR"
     gene            complement(4692..5318)
                     /locus_tag="VF276_19100"
     CDS             complement(4692..5318)
                     /locus_tag="VF276_19100"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX6609017.1"
                     /translation="MPAAPDPSDSVPATSAAPALPALTAGVRPPASARPEAVYLAGLA
                     PAGRRSMAARLRTVAGLLGAPSVDAVPWEQLRYAHVAAIRAALQERALAPASINTTLT
                     ALRGVAASAGNLGYLPVEELERIRQVKPVRGSRLPAGRAVPADELRTLLEACAADPSI
                     AGLRDAALIAVLYATGLRRGELAALHLADYSPAPPALTVRQGKGRAEH"
BASE COUNT          937 a         1737 c         1827 g          878 t
ORIGIN      
        1 gcagcagcaa gaagccaccg ggttgacctc gtgtggtagg tcaccaccga ttgtataacg
       61 caatcagaac cgcgcggctg ggccgcggta ggaaaggaag tgggtatggc taagcttgtg
      121 ttcggaatga accagtccct ggatggctac gtcgaccatc tggcgtttgc gcccagcccc
      181 acgctcttcc gccacttcat cgcggaggcg caggggcagg cgggcagtgt gtacgggcgc
      241 cgcctgtatg aggtcatgcg ctactgggac gacgatcatc cggaatggga tgccgaggaa
      301 caggccttcg cggcggcgtg gcggcaccag ccgaaatggg tcgtctcgcg ctcgttgccg
      361 gcggtcggcc ccaacgcccg gctggttgcg gatgatctgg cgggcgcgat ccgcgcgctg
      421 aaggccgagc gcgacgggga gatcgaagtt ggtggcccgg ccttggcgca cagcctcacc
      481 gaactgggcc tgatcgatga gtatcgcatc tacctgcacc ccgtcgtact gggtcatggc
      541 aagccatatt tcgccggacc ccggccgccg ctgcgcctta tggctcatga gcggattgcc
      601 gaggatgtga tccggttgac ctacgttcct gcgtagtcgc gggatccccc tggcccgttc
      661 cccctgatca acgtcgacct cgggctgccc gaggcaagcg gtgcggggcc ataagcgaac
      721 cagaacgcgg gggccggagg ggcgatcgag ttgaattggc cggatccgtt atcaatagag
      781 ggtagctagc agccggcgga tgggtccgtc cgcaaaattt tctccagcgc tcggcccttg
      841 gctaactcat cgatcaattt gtccaggtag cgaatttccc gcatcgtggg ttcggcgatc
      901 tcttccagcc gcaccccaca cacgacgcct ttaatcaagg cccgcgccgg attcaggtgc
      961 ggtgcctggg cgaaaaagtg ttcaaagtcg atctggttgg ccaactgcgc cgccagcgct
     1021 tccgcgctgt agccggtcaa ccaccgaata atggtatcca cttcggcttg cgtccggcct
     1081 tttttctccg ccttcgccac gtaatgggga tagacactgg ccacacttgt ggtatagatg
     1141 cgatgtttgg tcatgctacg tccttcttgg ctggggatgc tccaggatcg cgcggcggag
     1201 ccgccgctga tcgcgcgcac ccggcggatg cggtccgcgc agcggtgctg gctggcccgc
     1261 cagtcattct aggcggcggc gcttggccgg atgccatgag cgactcccgt aaccggctgc
     1321 gccgactcga cccaccccac cgctgtattg tacagcaatt cagccccgcg gtgcacatgc
     1381 gctccgccag ggccgcggct gctcgcagcg ggctgcgctg cttggagcac ctggacgaac
     1441 tcgaacgcgc gccggcgctg atcgaggtga tacacagccc ggcccgcacc agtctgcgct
     1501 accgtgtcgt cgagtagccg ggggggacgc gccgcgccgg tatgcgtcga cgaggccggg
     1561 caaatggggg acgccgggcg cgcgggcgag cgcgaagcgg ccccggtcgg aggcgcgcac
     1621 gggccgcccc tgcggtaccg ccgcgccgat cccgcggtcc tcctgcggga tcgcggcgct
     1681 gcaggccacc gcctgcagcc cgcccgcccc ggctgcgccg ccccgcgcgt ggcagaacca
     1741 cgcgcagagg ggcagcggct ggtctggccc gagcggcagg atcaggtggc gcgacatctg
     1801 ggcaaggtcg gcgcgaacga gccggcggaa tactggcaac accaattttg gctcagctac
     1861 gggacctcac ctattgccgc tcacccggcg cactcccggc gcaagagcgc gtaccggcgc
     1921 gcgtcgtaga aggtagcata ccgaataagt ggtatcccgt ggagtggagc actccacacg
     1981 ggttctggcg aattcgcgtg caaaacccgg cacggccgat cttgtgctat actcgccgtc
     2041 ggatggacgc gtcattgcag ccgggcgatc tggtgatctg gtggaagcgc gtgcccggta
     2101 gcccctatgt tgtgccgctg gccgcgaccg tgctcgcggt cacggcgcac cgcgtcaaaa
     2161 tccaggccga tgaggacggc cggccggtga tccgctacgt cccgccggcc cggctgcaac
     2221 cgcgtgaccc gccgaatcgc agggaaagga aggatcggtg atggcacacc accccgcccc
     2281 gccgcagcct gattcgcccg acctgctgcg gcgggggaat ggcagcgatc cggccaggag
     2341 cggagttttc tggcgagtgg cgcgcggctg gcgcagttcg ccgaactgac accgcctgag
     2401 caaacagagg aggatgcggc ggcgacgctg gcactaaccg cggaagagcg cgagtacctg
     2461 gcggcgagcc tggcggagca acagcgcctg gaagctgcag agcgggagcg ccatgcgcga
     2521 gagttgtcct tggagaagcg atctgccaac cgtctgcgct atctggtagc gggtctggta
     2581 gtcttcctgc tggtcgcggg ggggctagca gcctgggcgc tcacccagag tcaggtggcc
     2641 caggctaatg agcaagccgc ccgcgccagc ctggcccatg ccgatgccct gcggctggac
     2701 gctgatgtcg atagcttgat ctcctcgcac ggtccgaacg acctggctgc cctccttatc
     2761 gtccgctcgc tcaatttcga ctacacgccc caggccgacg tgcagctctc tgccgccact
     2821 aactggacgt ttcccgcccg ggagttcgtg ggccatacca atggggtgaa tgacgtggcc
     2881 atctccccgg acggcaagta cctggccacg gcgtccaccg atgctaccgc tgccctctgg
     2941 aaccttgcga cgggtaagca gttgcacgtc ttctccggct acggcggtag cgtcgtcact
     3001 gcgcgattct cgcctgacgg caagtatctc cttaccagct ccgaagacaa gagcgttcac
     3061 atctgggaca tagccacagg acaaagtgtg cgcgtcttcg cgggtaaccc tgacatcgtc
     3121 gatgcggccg tcttctcccc cgacggcagg cgcgtggccg cctccgtcga ggatggcatt
     3181 gccagggtgt gggatatcgc cagcggccag ctggtgctcc agatccgcaa ccccggatac
     3241 attggtgtgg gctactcacc cgacggcaag tacctggcga cagggggagt ggaccccagc
     3301 cacatcgccc gtatctggga tgcctccagc gggcaattgg tgcaaacctt caccggtcat
     3361 accgacaatg tcaacaatcc cgtgttctcg cccgacggca aatatctcgt tacgccgagt
     3421 gatgacggca ccgtgcgcat ctgggacagc gctactggca agaccctgca tgtactgatg
     3481 gggcataccg gcggcgccac cggggtcgcc ttctccccag gcggcaagcg gctggccacg
     3541 ggtagcgcag accgaatcgc gcggatatgg gatgtttcga ccggccagct tgtgcagacg
     3601 ttgagcggac acaaggacgg ggtcggcggt gttgccttcg ccccggacgg caaatggctc
     3661 gccactgcca gtgccgacca taccgcccgc ttatggcccg tccaggcagc cacgggaccc
     3721 cgcatcttcg ccgggcatac ggatgtggtg caacgcgcca ccttttcacc cgacggtaaa
     3781 tatatcgcca ctgccagcgt cgacaatacg gcccggctgt gggatgccgc cacaggcgcc
     3841 cagttacacc agttcactgg tcacacgggc tgggtagaca ccgctatcct ctccccggac
     3901 ggcaagacgc tgctcacatc cagtaaagac gccacagcgc ggctctggga cgtggcgacg
     3961 gggacgcagc taaggatctt ccgcggacac acggacggtc tcaatagcgc ctacttctct
     4021 cgggacggca agacaatagt gacggccagc gacgacaaga cagcgcggct ctgggacgta
     4081 gcgacgggga agcagctaag gatcttccgc ggacacacgg atgtcgtcaa caaggcggtc
     4141 ctcgtacctg acgccaagct ggtgctgacc ggctccgctg acggtacggt ccggctttgg
     4201 gatgctagca ccggcaagga agtgcgccgc tatacgggcc atgtaggccc cgtccgccct
     4261 agcgtatcac ccagtggcaa aatgatggtg accgccagcg acgacggcac agtgcggctg
     4321 tgggatgtgc agagcggcca ggaactgcgc cgctttggcg tgcctggtgt ggctatgcat
     4381 acagccgaat tctcgccgga cggccgtatg atcgccgccg gagggcgcga tgaggaggtc
     4441 caagtttggg atgtggagag cggccaggag ttgcggcgct tcaatgggca aggcggggaa
     4501 gtaagaagtg tggcattctc cggggacggc aagcagatac ttgtcgctaa cgccgacaag
     4561 accgcccggc tctggccggc tgattaccat gatactatcc gtgccctatg cggcgaattg
     4621 acgcgcgacc tgacacctga tgagcgcacc caatacggaa taactgatca ggggcccacc
     4681 tgcctagcac gctaatgctc agcacggcct ttgccctggc gcacggtcaa cgccgggggc
     4741 gccgggctgt aatcggccag gtgcagggct gcgagctcgc cccggcgcaa gccggtggcg
     4801 tagagcacgg cgatcagggc ggcgtcgcgc aagcccgcta tgctggggtc ggcggcgcag
     4861 gcttcgagca gggtccgtag ttcgtcggcc ggcacggcgc gcccggcggg cagccggctg
     4921 ccgcgcaccg gcttgacctg gcggatgcgc tccagttcct cgacgggcaa atagcccagg
     4981 ttcccggcgc tggcggcgac gccgcgcagg gcagtcaggg tggtattgat cgaggccggg
     5041 gccagcgcgc gctcctggag ggcggcgcgg atggcggcga catgggcgta acgcaactgc
     5101 tcccagggca cggcgtcgac ggacggggcc ccgagtagcc cggcgacagt gcgcaggcgc
     5161 gcggccatgg agcggcggcc ggcgggggct aggcccgcta ggtagacggc ttcgggccgg
     5221 gcactggcgg gcgggcggac gcccgccgtc aacgccggca gggccggcgc ggcgctcgtg
     5281 gccggtacag agtcggacgg atcgggcgca gcaggcatgg gcaaaccctt tcggcacgat
     5341 taaaaattag atccgtgaag gacacttcta gcaccaagc
//
LOCUS       DASUYC010001170         2151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_681764,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001170 DASUYC010000000
VERSION     DASUYC010001170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681764"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VF276_19105"
     CDS             <1..286
                     /locus_tag="VF276_19105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6609018.1"
                     /translation="RVPADDEGQARNRLAALREAGVQTGIWWYADHPTHYQGDARLAQ
                     RDAGEQLLETMADAVARAVRAIKADDVTPRLQDAFHRAAAAPGSLVPDGD"
     gene            305..>2151
                     /locus_tag="VF276_19110"
     CDS             305..>2151
                     /locus_tag="VF276_19110"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX6609019.1"
                     /translation="MMEEPVARYDQIDPTVEARVDDLLGRMTLAEKVGQLVQITPFAP
                     FDVAKMEEQARLAEAEGRPFVFEPEVRADLEELIRAGRVGSIFGTADVALINRCRRAA
                     VEGSRLGIPLIVGNDVIHGLRTVFPIPLAEASTWDPPLLEQAARIAAEEASATGTDWI
                     FAPMVDVARDPRWGRIAEGAGEDPYLGGVMAQARVRGFQSAGLASGQRIAACPKHYVA
                     YGGAEAGRDYNTVDISERTLRDVYLPPFKAAFDAGAGTVMSSFNEIGGVPATCNAFIL
                     TSVLRDEWQWAGMVVSDYEAVRELLAHGVAADLQDAARRSILAGLDMDMVSNAYADHL
                     AALVEAGAVPQAAVDEAVRRVLRMKIRLGLFEHPYTGEEIAGKVILRDDFREVALRVA
                     QESMVLLKNEDGLLPLAPDAGRVAVIGALADNRRELLGCWAFHGQPEDVETVLEGLSV
                     YLEGDRAPVYVPGYEVPAKPGEEAAVDIPAAVAAAREADVVVLVAGESADMSGEAHSR
                     AHLGLPGRQQELLDALAATGKPLVVVLMSGRPLVIPRVAEQATALLAAWHGGIRAGQA
                     IADILFGAANPSGKLTASWPRTEGQIPVYYAHKNTGRPVSGSGTTQFEEA"
BASE COUNT          314 a          724 c          827 g          286 t
ORIGIN      
        1 ccgggtgccg gcggatgacg aagggcaggc gcgcaaccgg ctggcggcgc tgcgcgaggc
       61 gggcgtgcag acgggcatct ggtggtacgc cgaccacccc acgcactacc agggcgacgc
      121 ccggctggcg cagcgcgacg cgggcgagca gttgctggag accatggccg atgcggtggc
      181 ccgcgccgtc cgcgcgatca aggccgacga cgtgacgccg cgcctgcagg acgcgtttca
      241 tcgcgccgcc gcggcacccg gcagccttgt cccggatggg gactgagccg gggtagggag
      301 gagcatgatg gaagagccgg tggcccgtta cgaccagatc gatcccaccg tcgaggcccg
      361 cgtcgacgac ctgctggggc gcatgacgct ggctgaaaag gtgggccagt tggtgcagat
      421 cacgcccttc gcgccgttcg atgtcgcgaa aatggaggag caggcgcggc tggccgaggc
      481 cgagggccgg cccttcgtat ttgagccgga ggtgcgcgcc gatctcgaag aactgatccg
      541 cgcgggccgg gtcggttcga ttttcggcac ggcggatgtg gcgctgatca atcgctgccg
      601 gcgggccgcc gtggagggat cgcgcctggg catcccgctg atcgtcggca acgacgtgat
      661 ccacggcctg cgcaccgtct tccccatccc gctggccgag gcgtccacct gggacccgcc
      721 gctgctggaa caggccgcgc gcatcgccgc cgaagaggcg tcggccaccg ggaccgactg
      781 gatcttcgcg ccgatggtgg acgtggcgcg cgacccgcgc tggggccgca tcgccgaggg
      841 cgcgggcgag gacccctacc tgggcggggt gatggcccag gcgcgagtgc gcggcttcca
      901 gtccgccggg ctggcgagcg ggcagcgcat cgccgcctgc cccaagcact acgtggccta
      961 cggcggcgcg gaggcgggcc gcgactacaa caccgtggac atctccgagc gcaccctgcg
     1021 cgacgtgtac ctgccgccct ttaaggccgc cttcgacgcc ggggccggca cggtcatgag
     1081 ttcgttcaac gagatcggcg gcgtgcccgc gacctgcaac gccttcatcc tgaccagtgt
     1141 gttgcgcgac gaatggcagt gggcgggcat ggtggtcagc gactacgagg cggtgcgcga
     1201 actgctggcc cacggcgtgg ccgccgacct gcaggacgcc gcccgccgca gcatcctggc
     1261 cgggctggat atggacatgg tcagcaacgc ctacgccgat cacctggccg cgctggtgga
     1321 ggcgggcgcg gtgccgcagg cggcggtgga cgaggcggtg cggcgcgtgc tgcggatgaa
     1381 gatccggctc ggcctcttcg agcaccccta taccggcgag gagatcgccg gcaaggtgat
     1441 cctgcgcgac gacttccgcg aggtggcgct gcgcgtggcc caggagtcga tggtgctgct
     1501 caagaacgag gacggcctgc tgccgctggc gccggacgcc gggcgggtcg ccgttatcgg
     1561 tgcgctggcc gataaccggc gcgaactgct cggctgctgg gcattccacg ggcagccgga
     1621 ggacgtggaa accgtgctcg aagggctgag cgtctacctg gaaggggacc gggcgccggt
     1681 gtatgtgccg ggctacgaag tgccggcgaa gccgggcgag gaggccgcgg tggatatccc
     1741 cgccgccgtc gccgcggcgc gcgaggccga tgtggtggtg ctggtcgcgg gcgagagcgc
     1801 cgacatgagc ggggaggccc actcgcgggc gcacctcggc ctgccggggc gccagcagga
     1861 actgctcgac gccctggccg ccaccggcaa gcccctcgtc gtcgtgctga tgagcgggcg
     1921 gcccctggtc atcccgcgcg tggccgagca ggcgacggcg ctgctcgccg cctggcacgg
     1981 cggcatccgc gccgggcagg ccatcgccga catcctcttc ggcgccgcca atccgtccgg
     2041 caagctgacg gcaagttggc cgcgcacgga ggggcagatt cctgtatatt acgcgcacaa
     2101 aaacacgggg cgcccggtca gcggcagcgg cacgacacag ttcgaggagg c
//
LOCUS       DASUYC010001171         2456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1830377, whole genome shotgun sequence.
ACCESSION   DASUYC010001171 DASUYC010000000
VERSION     DASUYC010001171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1830377"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..1154
                     /gene="aroB"
                     /locus_tag="VF276_19115"
     CDS             36..1154
                     /gene="aroB"
                     /locus_tag="VF276_19115"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017910872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEX6609020.1"
                     /translation="MSRNIVVRTPEGTYPISIGTGLLRDGVELVDGINSGKQVAVATD
                     SHVGPLHAATAQAMLRRAGFNPALITMPAGEAHKNWASLDLLISGFAETGLDRSGWVL
                     ALGGGVVGDTVGLAAALYMRGVPVVQTPTSLLAMVDSSVGGKVAIDHPLGKNLVGAFK
                     QPAAVIADLTTLATLPPDEVSNGMAEVVKAAILDGPWNRSPVGADGRSPLLDLLERGA
                     APSEEMVALAIEVKKRIVEADPYEQNQRALLNLGHTFGHAFERLSNYTLKHGYGVAEG
                     MMVAAHLAERLGLALPALTTRTARLLSNYHLPLTWGRSLPPGTTPEQVLAATATDKKR
                     RDGRQRFVLARGIGDVHVEDHVPEAEVLAALAATRRDA"
     gene            1151..1600
                     /gene="aroQ"
                     /locus_tag="VF276_19120"
     CDS             1151..1600
                     /gene="aroQ"
                     /locus_tag="VF276_19120"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017307149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEX6609021.1"
                     /translation="MTHNILVLHGPNLNMLGRREPEVYGHTTLAEIDALLVQAAGERA
                     TLRTRQSNHEGALIDAIQEAYGWMSGILINPGAFTHYSYALRDALAGAAVPAVEVHLS
                     NVYAREPFRHTSVLAPVCRGQISGFGVDSYLLGLEALLRLLDRAPGP"
     gene            1621..2433
                     /locus_tag="VF276_19125"
     CDS             1621..2433
                     /locus_tag="VF276_19125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HEX6609022.1"
                     /translation="MPGLHDYHMHSYLCGHGEGVPAEYAARAVALELDEIGVSEHIPL
                     YWLPEAERDPELAMPMGNLPLYFELVDAARRAYPALPIRLGLEADYIPGHEDALRAIL
                     EAHPWDYVYGSVHFLGAWGMDDPRYVAEYDRRDINALYEEYFRTVEAAARSGLFDIMA
                     HLDLVKKFGHRAMQDLGPLYARVAQAMAESGVCVEVNTAGLLKPVTEIYPQVDLLRAL
                     YRAGVPITLGSDAHAPHLVGYAFAEAVALLREVGYERVVRFAGRARSYAALP"
BASE COUNT          392 a          846 c          850 g          368 t
ORIGIN      
        1 gtctacacag ggcaagtgaa ggagcgagac acgccgtgag tcgcaacatc gtcgtgcgga
       61 cacccgaagg cacatatccc attagcatcg gaaccggatt gctgcgcgat ggggtagagt
      121 tggtcgatgg cattaacagc ggcaagcagg ttgccgtggc gaccgacagc catgtcggcc
      181 cgctgcacgc ggcgacggcg caggccatgt tgcggcgcgc gggcttcaac ccggccctga
      241 tcaccatgcc tgcgggagag gcacataaga actgggccag cctcgacctg ctcatctccg
      301 gcttcgccga aaccgggctg gaccgtagcg gctgggtgct ggcgctgggt gggggcgtgg
      361 tcggcgacac ggtgggcctg gccgcggcgc tctacatgcg cggcgtgccc gtggtccaga
      421 cgccgaccag cctgctggcg atggtcgact ccagcgtggg cggcaaggtc gctatcgacc
      481 acccgctggg caagaacctg gtcggcgcgt tcaagcaacc cgccgccgtc atcgccgacc
      541 tgacgacgct ggcgacgctg ccgcccgacg aggtcagcaa cggcatggcc gaggtcgtga
      601 aggcggcgat tctggacggc ccctggaatc gcagcccggt gggcgccgac ggccgctcgc
      661 cgctgctcga tctgctggag cgcggcgccg cgccgtccga agagatggtg gccctggcga
      721 ttgaggtcaa gaaacgcatc gtcgaagccg acccctacga gcagaaccag cgcgcgctgc
      781 tcaacctggg ccacaccttc gggcacgcct tcgagcgcct gagcaactat acgctcaagc
      841 acggctacgg cgtggccgag ggcatgatgg tggccgcaca cctggccgag cgcctggggt
      901 tggcgctgcc cgccctgacc acacgcacgg cccgcctgct cagtaactac cacctgccgc
      961 tgacctgggg ccgcagcctg ccgcccggca ccacgccgga acaggtgctg gcggccaccg
     1021 ccaccgacaa gaagcgccgc gacggccgcc agcggttcgt gctggcgcgc ggcatcggcg
     1081 atgtgcatgt ggaggaccat gtgcccgaag ccgaggtgct ggccgcgctg gccgccacgc
     1141 gaagggacgc atgacgcaca atatcctggt cctgcacggg ccgaacctga acatgctggg
     1201 ccgccgcgag ccggaggtct acgggcacac cacgctggcc gagatcgacg cgctgctggt
     1261 tcaggcggca ggcgagcgcg ccacgctgcg cacgcggcag tcgaaccacg agggcgcgct
     1321 gatcgacgcc atccaggagg catacggctg gatgagcggc atcctgatca accccggcgc
     1381 cttcacccac tacagctacg ccctgcgcga cgccctcgcc ggggccgccg tgcccgccgt
     1441 cgaagtccat ctgtcgaacg tctacgcccg cgagccgttt cgccacacct cggtgctggc
     1501 gcccgtctgc cgcgggcaga tcagcgggtt cggcgtggac agctacctgc tgggcctgga
     1561 ggcgctgttg cgcctgctgg accgcgcgcc cggcccctga gcgaaaggat cacgccctct
     1621 ttgcccggac tccacgacta ccatatgcac tcgtacctct gcggccacgg cgaaggcgtg
     1681 cccgccgagt acgccgcccg cgctgtcgct ctcgaattgg acgagatcgg cgtgtcggag
     1741 catatcccgc tctactggct gccggaggcc gagcgcgacc cggagctagc catgccgatg
     1801 ggcaacctgc cgctctactt cgagctagtc gatgccgcgc ggcgggccta tccagccctg
     1861 cctatccgcc tgggcctgga agccgattat atccccggcc acgaagacgc gttgcgggcg
     1921 attctagagg cgcacccctg ggattacgtg tacggctcgg tgcatttcct gggcgcctgg
     1981 ggcatggacg acccgcgcta cgtggccgag tacgaccgcc gcgacatcaa cgcgctgtat
     2041 gaggaatatt tccgcacggt cgaggcggcg gcgcgtagcg gcctgttcga tatcatggcc
     2101 catctcgacc tggtgaagaa gttcggccac cgggccatgc aggacctcgg cccgctctat
     2161 gcgcgcgtgg cccaggcgat ggccgagagt ggcgtctgtg tggaggtcaa cacggcgggg
     2221 ttgctcaagc cggtgaccga gatctacccg caggtcgatc tgctgcgcgc gctctaccgg
     2281 gcaggcgtgc ctattacgct ggggtccgac gcccacgcgc cgcacctggt gggctatgcc
     2341 ttcgccgagg cggtggcgtt gctgcgcgaa gtcggctacg agcgggtggt gcgcttcgcc
     2401 gggcgcgccc ggtcttacgc ggcgctgccc tgaaagaacg cggccaggta ggggcg
//
LOCUS       DASUYC010001172         2441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1139042, whole genome shotgun sequence.
ACCESSION   DASUYC010001172 DASUYC010000000
VERSION     DASUYC010001172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139042"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..1408)
                     /locus_tag="VF276_19130"
     CDS             complement(185..1408)
                     /locus_tag="VF276_19130"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HEX6609023.1"
                     /translation="MKQLAILGWYGSPNLGDEAELAAILAACARLPQPPRVVAFSVHP
                     ARTLTQHGGYPGGLRALPRNPFAPATLRALRGSAALILGGGGLIQDQTSIYNLPLFTL
                     FALVAAGLRRPLQWWGVGVEPLVTPLGRAQARLLVRLAAPGAVGLRDENSRRLLRRAG
                     VPARALRVSADPAVTIRPAPLAEIAGLLPPDAGVAARPCVAFCVRDLPNNPRGAGPGY
                     LLPVALQKRLGLGGGDRATHARRAAAFYALLACAADHLVEQHGARVLFVPFWPGRDDA
                     VAVTILAQMRHPDAAVLLDAPLTPAQARALLGMMDLVVAMRLHALIFAATAGVPVLGF
                     GYAQKVPAFLGRLGQGVRALDPATLTWPQLQAALDSVWAERDAIRPALRSAAVRLQAA
                     AAEDAAVAARLLGAA"
     gene            complement(1405..>2441)
                     /locus_tag="VF276_19135"
     CDS             complement(1405..>2441)
                     /locus_tag="VF276_19135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6609024.1"
                     /translation="TISWGRSTIFLPFFLLYALLRALVIIPFNRRIQIIHLSDALLAP
                     LGLLLKTLFRVPVVVTVHGLDITFDNGFYQWLVPRCLVHMDKLICVSGYTRDQCLRRG
                     IPAALCEVIPNGVNMSEFACNFSEEGVAAVEARVGSRLRGRKALLTVGRLIERKGVVH
                     FLDSILPRVLARCPDTCYLIVGEGPLHEVIETRIASLHLDDHVVVLGQVDDATLKATY
                     HLADLFVMPNVPVPNDIEGFGLVALEAGAAERYVVASRLDGIPEAVVSGKNGILVDPD
                     DTQGFVDAIVRLLEDDAYRAAHGRQAREFVRAHYSWDIIARRYNDLFRDVVQAALARG
                     ELRRGRRARF"
BASE COUNT          402 a          910 c          840 g          289 t
ORIGIN      
        1 aaaccggcct gccgccccac cggtcgcccg ccctcgcgca tcccgcccat gtaggggcca
       61 gtttcaaacc ggcctgccgc caccccgccg ctagtccgcc ctcgcgcatc ccgcccatgt
      121 aggggccggt ttcaaaccgg cccgcgccgc cctcaccgag gcgaatcacc aatcgccgtc
      181 ttccctacgc cgcgcccagc agccgcgccg cgaccgccgc atcttccgcc gctgccgcct
      241 gcagccggac cgccgcgcta cgcagcgccg ggcggatagc atcccgctcg gcccacaccg
      301 agtcgagcgc ggcctgcaac tgcggccagg tcagcgtggc cgggtcgagc gcccgcacac
      361 cctggccgag gcgccccagg aacgcgggca ccttctgcgc gtagccgaac cccagcaccg
      421 gcacccccgc cgtcgccgcg aagatcagag cgtgcaaccg catcgccacc accaggtcca
      481 tcatgccgag cagcgcgcgc gcctgcgccg gagtcagcgg cgcgtcgagc agcacggcgg
      541 cgtcggggtg gcgcatctgc gcgaggatcg tcaccgcgac ggcatcatcg cggcccggcc
      601 agaagggcac gaacagcacc cgcgcgccgt gctgctcgac caggtgatcg gcggcgcagg
      661 ccagcagcgc gtagaaggcc gccgcgcgtc gggcatgcgt cgcgcggtcg ccgccgccca
      721 ggcccagccg cttctgcaag gccaccggca agagatagcc cggcccggcc ccgcgtgggt
      781 tgttgggcag gtcgcgcacg cagaaagcca cgcacgggcg ggccgccaca ccggcatccg
      841 gcggcaacag cccggcgatc tcggccaggg gcgcgggccg gatggtcacg gcggggtcgg
      901 cgctgacgcg cagggcgcgg gccgggaccc cggcgcggcg caacaggcgg cgcgagttct
      961 cgtcgcgcag acccacggcg ccgggcgcgg ccagccgcac cagcagccgc gcctgcgccc
     1021 gccccagcgg cgtcaccagc ggctccacgc ccacacccca ccattgcagc gggcgccgca
     1081 ggcccgcggc caccagggca aagagcgtga acagcggcag gttgtagatg ctcgtctggt
     1141 cctggatcag gccgcccccg ccgaggatga gcgccgcgct gccgcgcaag gcgcgcagcg
     1201 tggccggagc gaaggggttg cgcggcaggg cgcgcagccc gccgggatag ccgccgtgct
     1261 gggtcagggt gcgcgcgggg tggacggaga aggcgaccac gcgcggcggc tggggcagcc
     1321 gcgcgcaggc ggccaggata gcggccagtt cggcttcatc ccccaggttg gggctaccgt
     1381 accagccgag aatcgccagt tgcttcaaaa gcgggctcgg cgcccccggc gcagttcgcc
     1441 acgggccagg gccgcctgca ccacgtcgcg gaacaggtcg ttgtagcgcc gggcgatgat
     1501 gtcccagcta tagtgcgcgc gcacgaactc gcgcgcctgg cgcccgtgcg cggcccggta
     1561 agcgtcgtct tccagcagcc gcacgatggc gtccacaaag ccctgcgtat catcggggtc
     1621 caccaggatg ccgttcttgc ccgacacgac ggcttccggg atgccgtcga gccgcgaggc
     1681 caccacgtag cgctcggcgg cgcccgcttc cagcgccacc agtccgaagc cctcgatgtc
     1741 gttgggcacg ggcacgttgg gcatcacgaa caggtcggcc aggtgatagg tcgccttcag
     1801 cgtggcgtcg tccacctgtc ccagcaccac cacgtgatcg tccaggtgca ggctggcgat
     1861 gcgcgtctct atcacctcgt gcagcggccc ctcgccgaca atcaggtagc aggtatcggg
     1921 gcagcgggcc agcacccgcg gcaagatgga gtcgaggaag tgcactacgc ccttgcgctc
     1981 gatcaagcgg cccaccgtca gcagcgcttt gcgcccgcgc agccgcgacc ccacccgcgc
     2041 ttcgacggcg gccacgccct cttccgagaa gttgcaggcg aattcgctca tgttgacgcc
     2101 gttggggatc acctcgcaca gcgcggcggg aatgccccgg cgcaaacact ggtcgcgcgt
     2161 gtagccgctg acgcagatca gcttgtccat atgcaccagg cagcgcggca ccagccattg
     2221 gtagaagccg ttgtcgaagg tgatatcgag gccgtgaacg gtgacgacca ccggcacgcg
     2281 aaacagcgtc ttgagcagca agcccagcgg cgccagcaac gcgtcgctca ggtggatgat
     2341 ctggatgcgc cgattaaagg ggatgatgac cagcgcccgc agcagggcat agagcagaaa
     2401 aaacggcaga aaaatggtgg aacggcccca cgagatcgtg g
//
LOCUS       DASUYC010001173         4272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1600816, whole genome shotgun sequence.
ACCESSION   DASUYC010001173 DASUYC010000000
VERSION     DASUYC010001173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1600816"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..904)
                     /locus_tag="VF276_19140"
     CDS             complement(212..904)
                     /locus_tag="VF276_19140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6609025.1"
                     /translation="MFIVCPDCGADQQEVGKYCENCGRLLTAADAQAPAPVAAPATGA
                     PWNAAPAATPVASDPAPWAAPLPGATQAIPPGSARFAIVRNGQANPAEGFTITQEGEF
                     LVGRPNIEAGSGVDVDLRQWVQPLDLNGQKQYLIHRRQCFLGIGTDGVVTIRPCPGAE
                     ADTLVKPAGQPGFIPLNQFSTVRALRPDSTFPLQPGDQVYMGDPDAVRYYQTGDPTAA
                     GTYVVLELMPGA"
     gene            1046..1981
                     /locus_tag="VF276_19145"
     CDS             1046..1981
                     /locus_tag="VF276_19145"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX6609026.1"
                     /translation="MTETNTVRVWSKSIPSRLHPDRDEDLAWSAANGLAHAVIDGMGS
                     ARRRTPHGEIGGEHAARMIGQVLDARLQNLPANLTVPAARELLAAVVREAGERVFHDV
                     NAAGRIPPDEIPPGKTAADVLAAAVMTATILCEGGRRAVISQNGDTRGYLYSNRELLL
                     LTEDQDAVGSDLEQQVLTPDQAYTVEETIDNFDGHDLGAMDPMVRRYYAQRFLVFGQI
                     GDALPVPAPALTVIQLRPRDLLLLTSDGVHDNLTRREIADGLELIDPAAALVDRADAR
                     SAERALPDAADLNQPYNYRAHQDDITAIVIKIDWV"
     gene            2239..3288
                     /locus_tag="VF276_19150"
     CDS             2239..3288
                     /locus_tag="VF276_19150"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX6609027.1"
                     /translation="MLAHDKRGKDAAPRLDDRALTAVDGTDAAGRLVVDVAHKVEGPA
                     VPMVAGRMSRPWRDLGWAILYMAPALAIFIAFTFVPFVRSIWLSFFITDQTGSPARFN
                     GIQYYLRFLNLDGSGRDEYLHSVLTTIEFAVLVVPLGIAVAVGLAQLATARVRAIQIF
                     RTIFTSTVAISVASAAVIWALIYNPSIKVTNWVVDLLNLQTEGVLLDPATALPAVAFM
                     TIWTGLGFNFIIALAGMQGIPADLYESSTLDGASAWQAFRHITLPLLTPTLLFLLIIN
                     TINSFQAFTQFNVLIANEGPNGSTNVMVFALFTAFFKENRYGFASTIAVVLFVVLLVL
                     SLIQFRLLDRRVHYQ"
     gene            3285..4157
                     /locus_tag="VF276_19155"
     CDS             3285..4157
                     /locus_tag="VF276_19155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016821186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX6609028.1"
                     /translation="MSAQPIAASYEYRSGTQRVRDWLKTTGLYILLILAALILLFPLI
                     FSLSLSLQGPTIAPTLIPNFNKLDWGVFREAFNEEPLLSRWIVNSFVVSLAVTIGQLI
                     TSALAAYALATFNFPAKTLFFFLFLGTLMIPWEATIVPNYLTVVQLHWKDSYQGLVAP
                     FLASGFGIFLLRQYFLTIPRDLYEAAVIDGCGRFRYLWSILLPLSRPALGTLAVYAFL
                     NTYNQYYWPLLVTDSPEWRTTQVGITAFRSSEIALYNLQMAGTIIVMLPTLILLVLGQ
                     RQLVRGLTSGALKG"
BASE COUNT          713 a         1487 c         1335 g          737 t
ORIGIN      
        1 tctgcatccg agcgggttac agcctccaca gtgattccgc cggcggcagg ccggtttgaa
       61 accggcccct acaacaccgc cgtgcacgcc aggccggttt gaaacaggcc cctacaacgg
      121 ccaacgcgca acagcaggcc agtttgaaac cgggcgccta cagcggcgaa ccggcggatt
      181 ataagcggcc ctacacggcg ccggcggact attacgcccc cggcatgagt tccaggacca
      241 cgtaagtgcc cgcggcggtg gggtcgccgg tctggtagta gcggaccgcg tccgggtcgc
      301 ccatgtagac ctggtcgccg ggctggagcg ggaaggtcga gtcgggccgc aaggcgcgga
      361 cggtgctgaa ctggttcagc gggatgaagc cgggttgccc ggcgggctta accagcgtgt
      421 cggcttcggc gccgggacac gggcggatcg tgaccacgcc gtcggtgccg atgcccagga
      481 aacactgccg gcgatggatc aggtactgct tctggccgtt caggtccagc ggctgcaccc
      541 actggcgcaa atcgacatcc acccccgacc ccgcctcgat gttgggccgg cccaccagga
      601 attcgccttc ctgggtgata gtgaagccct ccgccgggtt cgcctgcccg ttgcgcacga
      661 tggcgaagcg cgcgctgccc ggcgggatcg cctgcgtggc gccgggcagc ggtgcggccc
      721 acggcgccgg gtcactcgcg accggcgtgg ccgccggggc ggcgttccag ggcgcgccgg
      781 tcgcgggcgc cgccacgggt gcgggcgcct gggcatcggc cgcggtcagc aaccgcccgc
      841 aattctcgca atatttgccg acttcctgct ggtccgcacc acaatcggga cacacaataa
      901 acacgaatcg atcctccttg ccggggcccc gcgcttccca ctcgctcaat ctacgcaggg
      961 cagcgtctca ggttgcaggg caattatact gctgattccg cctgtttgca ttataatggc
     1021 tgcagcaacc tgtaaggagc catccatgac cgaaaccaac accgtgcggg tctggtccaa
     1081 aagcatcccc agccgcctgc atccggatcg cgatgaagat ctggcctggt cggcggccaa
     1141 cggcctggct cacgccgtga tcgacggcat gggcagtgcc cggcgccgca caccgcacgg
     1201 cgagatcggc ggcgaacacg cggcgcgcat gatcgggcag gtgctcgacg cccgcctgca
     1261 aaacctgccc gccaacctga ccgtgcccgc cgcccgcgaa ctgctggccg ccgtggtgcg
     1321 cgaggccggt gagcgggtct tccacgacgt gaatgcggcg ggccgcatcc cgccggacga
     1381 gatcccgccc ggcaagacgg ccgccgatgt gctggccgcc gccgtgatga ccgcgacgat
     1441 cctctgcgag ggcgggcgcc gcgccgtgat cagccagaac ggcgataccc gcggctatct
     1501 ctattccaac cgcgaactgc tgctgctgac cgaggaccag gacgccgtgg gctcggatct
     1561 cgaacaacag gtcctgacgc ccgaccaggc ctacaccgtc gaggagacca tcgacaactt
     1621 cgacggtcac gacctgggcg ccatggaccc catggtgcgc cgctactacg cgcaacgctt
     1681 cctggtcttc ggccagatcg gtgacgcgct gcccgtgccc gccccggcgc tgaccgtcat
     1741 ccagttgcgc ccgcgcgatc tcttgctgct gaccagcgac ggtgtccacg acaacctgac
     1801 ccggcgcgag atcgccgacg gcctcgaatt gatcgacccc gccgccgcgc tggtggaccg
     1861 cgccgacgcc cgctcagccg agcgcgctct gcccgacgcc gccgatctca accaaccgta
     1921 caactaccgg gcgcaccagg acgatatcac ggccattgtg atcaagatcg actgggtgta
     1981 gaatcgcagt aggccaggcc gggcagttgc acgaaccggc caactatgct agacttggcc
     2041 cggttgttac gacacgactg cctatcgtag cgcgccgcgg aaactcgcgg caccgccggt
     2101 taacgccgcg gcgtgaagct accatcgcgg cctgagcgca ccagcattcc acgacgaccg
     2161 ccgcccgcgc cccgcccgcg gggccgttcc gttcggacgg atgcggatcg atccggagat
     2221 agaggccaca gacgacctat gctcgcacat gacaagcgcg gcaaagatgc cgccccccgg
     2281 ctcgatgacc gggcgctcac cgccgtggac ggcaccgatg ccgcgggccg cctggtggtc
     2341 gatgtggccc acaaggtgga ggggcccgcc gtgcccatgg tggcgggccg gatgagccgc
     2401 ccctggcgcg acctcggctg ggctatcctg tatatggccc cggcgctggc gatcttcatt
     2461 gccttcacct tcgtgccctt cgtgcgctcg atctggctca gctttttcat caccgaccag
     2521 accggcagcc cggcgcgctt caacggcatc cagtactacc tgcgcttctt gaacctcgac
     2581 ggcagcgggc gcgacgagta cctgcacagc gttctcacca ccatcgagtt cgccgtgctg
     2641 gtggtgccgc tgggcatcgc cgtcgccgtg ggcctggccc aactggcgac ggcgcgggtg
     2701 cgcgcgatcc agatcttccg cacgatcttc accagcaccg tcgccatctc cgtcgccagc
     2761 gctgccgtca tctgggcgct catttacaac cccagcatca aagtgaccaa ctgggtagtc
     2821 gatctcctga atctgcaaac cgaaggtgtg ctgctcgacc cggccaccgc cctgcccgcc
     2881 gtcgccttca tgaccatctg gaccgggctg gggttcaact tcatcatcgc gctggccggc
     2941 atgcagggca tcccggcgga cctctacgaa agcagcaccc tggacggcgc gagcgcctgg
     3001 caggcgttcc gccatatcac cctgccgctg ctcacgccga cgttgctgtt cctgctgatc
     3061 atcaacacga tcaactcgtt ccaggcgttc acccagttca atgtgctcat cgccaacgag
     3121 gggccgaacg gctcgaccaa cgtcatggtg ttcgcgctgt tcaccgcctt ctttaaggag
     3181 aatcgctacg gcttcgcctc gaccatcgcg gtcgtgctgt tcgtcgtgct gctggtgctg
     3241 agcttgatcc agttccgcct gctggacagg agggtccact atcaatgagc gcccaaccta
     3301 tcgccgcgtc ctatgaatat cggtccggca cgcagcgggt ccgcgattgg ctgaaaacca
     3361 ccggcctgta tatcctgctg atcctggccg cgctgatcct gctgttcccg ctgatcttct
     3421 cgctgtcgct gtcgttgcag gggccgacca tcgcgccgac cctgatcccc aacttcaaca
     3481 agttggattg gggcgtgttc cgcgaggcgt tcaacgagga accgctgctg agtcgctgga
     3541 tcgtgaactc gttcgtggtc tcgctggccg tcaccatcgg gcagttgatc acatcggcgc
     3601 tggcggcgta tgcgctggcg accttcaact tcccggccaa gacgctgttc ttcttcctgt
     3661 tcctgggcac gttgatgatc ccctgggaag cgacgattgt gcccaattac ctgacggtgg
     3721 tgcagttgca ctggaaggac agctatcagg gcctggtcgc gccgttcctg gccagcggct
     3781 tcggcatctt cctgctgcgg cagtacttcc tgaccatccc gcgcgacctg tacgaggccg
     3841 cggtgatcga cggctgcgga cggttccgct acctgtggag catcctgctg ccgttgtcgc
     3901 gcccggccct gggcaccctg gccgtgtacg cctttttgaa cacctacaac cagtactatt
     3961 ggccgctgtt ggtgaccgat agcccggaat ggcggaccac gcaggtcggc attacggctt
     4021 tccggtcgtc ggagatcgcc ctctataacc ttcagatggc ggggaccatt atcgtgatgc
     4081 tgccgaccct gatcctgctg gtgctgggcc agcggcaact ggtgcgcggc ctcacgagcg
     4141 gcgcccttaa gggctgaacc acaatctaca ggacccacgg aaccaaagga ggaaacatga
     4201 ctgtgcaacc gatgggcagt acgcatgagt cgcgcgcgag gaagcgcaag gggccgaagc
     4261 cggtcgagcg gc
//
LOCUS       DASUYC010001174         2433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1870404, whole genome shotgun sequence.
ACCESSION   DASUYC010001174 DASUYC010000000
VERSION     DASUYC010001174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1870404"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..515)
                     /locus_tag="VF276_19160"
                     /pseudo
     CDS             complement(57..515)
                     /locus_tag="VF276_19160"
                     /inference="COORDINATES: protein motif:HMM:NF033580.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(479..868)
                     /locus_tag="VF276_19165"
     CDS             complement(479..868)
                     /locus_tag="VF276_19165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX6609029.1"
                     /translation="MLTELTDAEWEIVVEAFRAARSRRGGRGRDDRKFLEALHYFTVH
                     NITWRALPREFGKWNSIWKRFWRLSQRGVFEAMLALLAETSGTAHLVQMVASTIVRAH
                     VSAAGAKGGRRARHSAARAADFQPKFT"
     gene            1028..2230
                     /locus_tag="VF276_19170"
     CDS             1028..2230
                     /locus_tag="VF276_19170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HEX6609030.1"
                     /translation="MSGASTEERLELWASSLRQVKGRMRPLFTQDRVAASAGLFLDGL
                     LGEERRKTGWMRAEAAGDPGPWRQQAILGRGRWDADALRDIVREYVVETLAEPDAVLV
                     VDETGFLKQGKASCGVARQYTGSAGKITNCQIGVFAAYVSGRGHAFIDRALYLPRAWA
                     DDPARRAAAHVPQEIAFATKPHLARAMVERAIAAGVPFAWVVGDSIYGVSEVEMALRR
                     AGKGYVLGVNSNSQFNSWGGKPPVAGTAAEIAGTLDPSAWRQLSAGTGAKGERLFDWA
                     YLELADLEAAEYNESLSGLWTRGLLIRRNIADGDLAFFTTWCPAGTGMETLVAVEGRR
                     WAIEDGFETAKNELGLDHNETRSWHGWHRHVSLVMLAFAMLAAIRRQANTPAPPKSSL
                     RMIRKSRR"
BASE COUNT          413 a          754 c          819 g          447 t
ORIGIN      
        1 gatcgccgcc gcggtgcgcg aggtacacgg ttacgacagg tgagccggcc ccggaactag
       61 gccgtgtgga cggattttgc ccggataaag gtgcaagcga gggagatgaa ggcggcaaag
      121 ttctgcgcgg ttttctcgca gcgcaacgcg atgcgtttga agcgcttgag cttgccgatg
      181 gtctgctcga tgcgggcgcg gcctttgtag agggtgcttg ggtagagggt gcttgggtag
      241 agggtgcttg gaaagaattt cggcttggcg gtggcattgc tgcggtacgg gatgacggga
      301 cagatgccgc gccggcgtgc cgctttgcga tttgccttgc tgtcgtagcc cttgtcgccg
      361 atcaccgccc ggggcgtgat gtccgggccg atgtcgagca gggtctcgaa attacggctg
      421 tcgctggctt ggccgccggt caggtggaag gccagcggca gaccatcgaa atcgcacttc
      481 aagtgaattt tggttgaaaa tccgccgcgc gagcggccga gtgcctggcc cttctgcccc
      541 cttttgcgcc ggccgccgag acatgagcgc gcacgattgt cgaagcgacc atctgaacca
      601 agtgcgcggt gccgctcgtc tcggccaaga gcgccagcat cgcctcgaaa acaccccgct
      661 ggctcagtcg ccagaaccgc ttccagatgc tgttccactt gccgaactct ctcggcaagg
      721 cgcgccaagt gatattgtga accgtgaaat aatgcagcgc ttccaggaat ttgcggtcgt
      781 cccgaccgcg cccgccacgg cgcgagcgcg ccgcccggaa cgcttcgaca acaatctccc
      841 actcggcatc cgtcagctcg gtcagcatga tcggctcctc ccagccaacc ctgtcccaac
      901 cactgatgcc ctccgatgta atgcccctta ctttctattc acccccttcc gtccacacgg
      961 cctagcacta cagttgcaat tttgacgagg ggttctgatc ttctcccgga tggttgggag
     1021 aggatggatg agcggcgcat caaccgagga gaggctggag ctttgggcgt cgtcgctgcg
     1081 gcaggtgaag gggcggatgc gcccgttatt cacgcaggac cgggtagcgg cctcggccgg
     1141 tctgtttctt gacgggctgc ttggggagga gcggcgcaag acgggctgga tgcgggccga
     1201 ggcggcgggt gaccccggtc cctggcggca gcaggcgatc ctggggcggg gccgctggga
     1261 tgccgacgct ctgcgcgata ttgtacgcga atacgtggtt gagacgcttg ccgaaccgga
     1321 tgccgtgctg gtggtggacg agaccgggtt tctaaaacag ggcaaggctt cctgcggcgt
     1381 ggctcggcaa tacaccggct cggcaggcaa gatcaccaac tgccagatcg gcgtgttcgc
     1441 cgcctatgtc tcgggccggg ggcacgcctt tatcgaccgg gcgctctatc tgccccgggc
     1501 ctgggccgac gatccggcgc gccgggcggc agcgcacgtg ccgcaggaga ttgcctttgc
     1561 gaccaagccg cacctggcgc gcgccatggt cgagcgggcc atcgcggcgg gggtgccatt
     1621 tgcctgggtg gtcggcgaca gcatctatgg ggtgagcgag gttgaaatgg ccctgcgccg
     1681 ggccggcaaa ggctacgtgc ttggcgtcaa cagcaatagc cagttcaact cctggggcgg
     1741 caagcctccc gtggccggga ccgcggccga gatcgccggc accctcgatc cctctgcctg
     1801 gcgtcagttg tcggccggga ccggagccaa gggcgaacgg ctgttcgact gggcctacct
     1861 ggaactggcc gatcttgaag cggctgaata caacgagagc ctgtccggtc tgtggaccag
     1921 gggccttctg atccgccgca atattgctga tggcgacttg gcctttttca ccacctggtg
     1981 ccccgccggc accggaatgg aaaccctggt ggcggtcgag ggccggcgct gggcgatcga
     2041 ggacggcttc gagacggcca agaacgagtt gggtctcgac cacaacgaga cccgctcctg
     2101 gcacggctgg catcggcacg tctcgctcgt catgctggcc tttgctatgc tggccgccat
     2161 ccgccgccaa gccaacacgc ccgcgccccc aaaaagcagc ctgaggatga tccgcaagag
     2221 ccgccgttga tctgctggtc ggtgcaggaa ctccgccgcg tggcttgccg cttggcgcag
     2281 caccagatcc aacccgccgc cgtcatcgcc tgggccctct ggcgacgggc ccaccaagcc
     2341 gccgcacaac aggctcacat caagttaaaa acgcaactgt agtgctaggg ctgggcgatt
     2401 gtattccgat cgacaatcgg aggctttgcg atg
//
LOCUS       DASUYC010001175         4178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_454400,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001175 DASUYC010000000
VERSION     DASUYC010001175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454400"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1169
                     /locus_tag="VF276_19175"
     CDS             <1..1169
                     /locus_tag="VF276_19175"
                     /inference="COORDINATES: protein motif:HMM:NF027641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HEX6609031.1"
                     /translation="IRHGSVVSQLQSVVSRFTNQPAFLRASGKPVIFFWSPQALGGVA
                     AWQDVRRQVDPNHNQIWSVDTTDQAYLDVFDSIHLFSAGKWNAGTNVGAVDKQWRGIV
                     DNYNHRTGGARLWAAGVIPGWDESRVQPPRPSPKVFPRHDGAMYAESWQAAIASDPDW
                     ITITSFNEWFEGTQIEPSATYGTRYLDLTRQYATQWKGTPPPQPAPGPAPCTAGQFFP
                     QTGHSICSQVQGYWQQYGGLMQFGYPITDPLVEVSPTDGKPYTVQYFERARFELHPEN
                     RGTPYEVLLGLVGRQFHPVDPPVPLRPGTGHYFEQTGHNVSTGFYQYWQQHGGLFVNG
                     YPISEEFAERSSDGKMYTVQYFERARFEWHPENPAPYNVLLGFLGHQAWSLRGR"
     gene            1413..1619
                     /locus_tag="VF276_19180"
     CDS             1413..1619
                     /locus_tag="VF276_19180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6609032.1"
                     /translation="MNPEAKSVSFVLRFVYEEPPGDGDRRAGPWYSVVRHVQSNTERH
                     FTRWDDVVAFIEDYVNLDRESGHE"
     gene            1612..2631
                     /locus_tag="VF276_19185"
     CDS             1612..2631
                     /locus_tag="VF276_19185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012449129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HEX6609033.1"
                     /translation="MSEPSIGIAAVGTYIPRTYITAAEIAAETGIPEHVIASKFGLTR
                     KPVPGPDDHTNAMALWAAQDALAGSDIAPDEIDVVLCTTEEWKEYPLWTAGIKLAYDL
                     GARRAWAIDVQMRCGTTIGALKLARSLMLAEPDVKTVLIAGGYRNGDLVDYRNPRSRF
                     MINLSAGGGALLLRKGYPRNRLLGTSVIVDGSFSLDVIVPAGGTMEPLTAEAIAAGRN
                     RLDVPDPEGMKQRLDALSMQNFLRVIDEALAQSGHTRADIDYLNVLHMKRSAHDFVLR
                     ELGLREDQSFYLSDFGHIGQQDQALSIKKGLETGRLHDGDLMVMVAAGIGYAWGAGVV
                     QWGSN"
     gene            2616..3395
                     /locus_tag="VF276_19190"
     CDS             2616..3395
                     /locus_tag="VF276_19190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP reductase"
                     /protein_id="HEX6609034.1"
                     /translation="MGIELTGKVAVITGGANGIGRETALTFVRAGAAVAIWDMAEATG
                     HAVAEQIREAGGQALFRKVNVVDATEVAAAVSEVVQEYGRIDILINNAGITRDAQLVK
                     IQHQHVVGKMSEADFDAVIAVNLKGVFNCTQAVAPVMIAQGSGRIISASSVVGLYGNF
                     GQTNYVASKAGVIGMTRVWARELGKRGISVNAVAPGFIATEMTAGMPDSVLAGMVAHT
                     PLGRMGEPADVAAAYLFLASDEAAFINGAVLNVDGGLVIGT"
     gene            3407..>4178
                     /locus_tag="VF276_19195"
     CDS             3407..>4178
                     /locus_tag="VF276_19195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011959281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX6609035.1"
                     /translation="MYIGDWLARRDMLSPHKVALIDTLHDNRPITYREWNAAANQTAS
                     LLRDLGIGKGDRVAVLAMNCVEYLDIWFASGKLGAILQTLNWRLTPHELGGLLADGTP
                     KVLVYGPEFVAQIQAVRAGAASVRHWIALDPAGRAAPDDLVFTAREDYPATPPPEVEL
                     TWDDPWVICYTGGTTGLPKGAVLTHGNIAWNSINTVMSWGLALDDVGVLNSPLFHTGG
                     LNVFTAPLVHIGGTSIVCRQFDVDQVYDLLANAGVTIYF"
BASE COUNT          723 a         1461 c         1348 g          646 t
ORIGIN      
        1 ccatccggca cggcagcgtc gtcagccagt tgcaatcggt ggtcagccgc ttcaccaacc
       61 aacccgcctt cttgcgcgcc agcggcaagc cggtgatctt cttctggtcg ccgcaggcgc
      121 tcggcggcgt ggccgcgtgg caggatgtac gccggcaggt cgatcccaac cacaaccaga
      181 tctggtcggt ggacacgacc gaccaggcct acctggatgt gttcgacagc atccacctgt
      241 tcagcgcggg caagtggaac gccggcacca acgtcggcgc ggtcgacaag cagtggcgcg
      301 gcatcgtgga caactacaac caccgcaccg gcggcgcgcg tctgtgggcg gcgggcgtca
      361 tccccggctg ggacgagagc cgcgtgcagc cgccgcgccc gtcgcccaag gtcttccccc
      421 gccacgacgg cgctatgtac gcggaaagct ggcaggcggc catcgccagc gaccccgact
      481 ggatcacgat aaccagcttc aacgagtggt tcgagggcac gcagatcgag ccgagcgcga
      541 cgtacggcac gcgctatctc gacctgacgc gccagtatgc gacgcagtgg aagggcaccc
      601 cgcccccgca acccgcgccc ggcccggccc cctgcacggc gggccagttc ttcccgcaaa
      661 ccggccacag catttgcagc caggtgcagg gctactggca gcagtacggc ggcctgatgc
      721 agttcggcta cccgatcacc gacccgctgg tcgaggtcag ccccaccgac ggcaagccct
      781 acaccgtgca atacttcgag cgcgcccgct tcgagttgca ccccgagaac cggggcacgc
      841 cctacgaggt gctgctgggt ctggtgggcc ggcaattcca cccggtcgat cccccggtgc
      901 cgctgcgtcc cggcaccggc cactatttcg agcaaaccgg ccacaacgtc agcacgggct
      961 tctaccagta ctggcagcag cacggcggcc tcttcgtcaa cggctacccc atcagcgagg
     1021 agttcgccga gcgcagcagc gacggcaaga tgtacacggt gcagtatttc gagcgggccc
     1081 gcttcgagtg gcacccggag aaccccgcgc cctacaacgt gctgctcggc ttcctgggcc
     1141 accaggcctg gagcctgcgg ggccggtaag catccggcgc gttgcgccgg cgccgacccg
     1201 ccgtttcctg gcgtgctgct acgtcacgcc ggcccatggg gccggcgtga ccacatgcct
     1261 ggagaaaata cccgcctttc cctcgcccgc cccgttctga tccctgccgc tgtaaccgat
     1321 ttgtaaccgg gttgtacccg gccccctgta cactggtccc gcaacaccca ccggcgccgt
     1381 tcgcggcatc ataccctacc ctggagccgt ccatgaaccc ggaggccaag tcggtctcgt
     1441 ttgtcttgcg ctttgtctac gaggagccgc ccggcgacgg cgaccgccgg gccggtccct
     1501 ggtacagcgt cgtccgccac gtgcagtcga ataccgagcg ccatttcacc cggtgggacg
     1561 atgtcgtggc ctttatcgaa gattatgtaa acctcgatcg cgagtcgggc catgagtgaa
     1621 ccgagcatcg gcatcgccgc cgtcggcacg tatatccccc gcacctatat caccgccgcc
     1681 gagatcgccg ccgaaaccgg catcccggag cacgtcatcg cgagcaagtt cgggctaacg
     1741 cgcaagccgg tgcccggccc cgacgaccat accaatgcca tggcgctgtg ggcggcgcaa
     1801 gatgcgctgg ccggcagcga catcgctccg gacgagatcg acgtggtgct ctgcacgacc
     1861 gaggagtgga aagagtaccc gctctggacg gcgggcatta agctggccta cgaccttggc
     1921 gcgcgccggg cctgggccat cgacgtgcag atgcgctgcg gcaccaccat cggcgcgctc
     1981 aagctggccc gcagcctcat gctggccgag cccgatgtca agacggtgct catcgccggg
     2041 ggctatcgca acggcgacct ggtcgactac cgcaatcccc gctcgcgctt catgatcaac
     2101 ctgagcgcgg gcggcggcgc gttgctgctg cgcaagggct atccgcgcaa tcgcctgctg
     2161 ggcacctcgg tcatcgtgga cggttcgttc tcgctcgacg tgattgtccc ggcgggcggc
     2221 accatggaac cgctgacagc ggaagcgatt gccgcgggcc gcaaccggct cgacgtgccc
     2281 gatccggagg gcatgaagca gcgcctcgac gctctgtcga tgcagaactt cctgcgcgtg
     2341 atcgacgaag ccctggcgca gagcggtcac acccgcgccg atatcgacta cctcaacgtg
     2401 ttgcatatga agcgctcggc gcacgatttc gtgctgcgtg aactggggct gcgcgaggac
     2461 cagtcgttct acctgtccga cttcggccac atcgggcagc aggatcaggc gctatcgatc
     2521 aaaaaaggac tggaaaccgg ccggttgcac gacggcgacc tgatggtcat ggtcgccgcg
     2581 ggcatcggct acgcatgggg agcaggagtg gtgcaatggg gatcgaacta accggcaaag
     2641 tggcggtgat caccggcggg gccaacggca tcgggcgcga aaccgcgctc acctttgtgc
     2701 gggccggggc cgccgtggcg atctgggaca tggccgaggc caccgggcac gccgtcgccg
     2761 agcagatccg cgaggccggc ggccaggccc tcttccgcaa ggtcaacgtc gtcgatgcga
     2821 ccgaggtggc agccgccgtg agcgaagtgg tccaggagta tggccgcatc gacatcctga
     2881 tcaacaacgc gggcatcacc cgtgacgcgc aactggtcaa aatacaacac cagcacgtgg
     2941 tcggcaagat gagcgaggcc gattttgacg ccgtgatcgc cgtgaacctc aagggcgtgt
     3001 tcaactgcac ccaggccgtg gctccggtga tgatcgccca gggcagcggg cgcatcatca
     3061 gcgcctcgtc ggtggtcggc ctctacggca actttgggca gaccaactac gtggcctcca
     3121 aggccggggt gatcggcatg acccgcgtct gggcgcgcga actgggtaag cgcggcatca
     3181 gcgtgaacgc cgtcgcgccc ggcttcatcg ccaccgagat gacggcaggg atgcccgact
     3241 cagtgctggc gggtatggta gcccacaccc cgctggggcg catgggcgaa cccgccgacg
     3301 tggccgcggc ctacctgttc ctggcctccg acgaggccgc tttcatcaac ggcgccgtgc
     3361 tcaacgtgga cggcggcctg gtcatcggca cctgagaggc cctgccatgt acatcggcga
     3421 ttggttagcc cggcgcgaca tgctcagccc gcacaaagtg gccctcatcg atacgctgca
     3481 cgacaaccgg ccgatcacct accgcgagtg gaacgcggcg gccaaccaga cggcgagctt
     3541 gttgcgcgac ctcggcatcg gcaagggcga ccgtgtcgcc gtgctggcga tgaactgcgt
     3601 cgagtacctg gacatctggt tcgcctccgg caagctcggc gcgatcttgc agacgctcaa
     3661 ctggcggctc acgccccacg agctaggcgg cctgctggcc gacggcacgc cgaaggtgct
     3721 ggtctacggg cccgaattcg tcgcccagat ccaggccgtg cgcgcgggcg ccgcatcggt
     3781 gcgccactgg atcgccctcg acccggcagg ccgcgccgcg cccgacgacc tggtcttcac
     3841 ggcacgcgag gactaccccg ctacgccacc gccggaagtc gaattgacct gggacgaccc
     3901 ctgggtgatc tgctacaccg gcggcaccac gggcctgccc aagggcgccg tgctgaccca
     3961 cggcaacatc gcctggaact cgatcaacac ggtcatgagc tggggcctgg cgctcgacga
     4021 cgtaggcgtg ctgaattcgc cgctgttcca taccggcggg ctgaacgtgt tcaccgcgcc
     4081 gctggtccac atcggcggca cgtccatcgt ctgccggcag ttcgacgtgg accaggtcta
     4141 cgacttgctc gccaacgcag gcgtgacgat ctacttcg
//
LOCUS       DASUYC010001176         7126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_213581,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001176 DASUYC010000000
VERSION     DASUYC010001176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 7126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7126
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213581"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            319..846
                     /locus_tag="VF276_19200"
     CDS             319..846
                     /locus_tag="VF276_19200"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6609036.1"
                     /translation="MDNQGTTNGKIRVLIADDVECMRESLRTVLSLDSSLEIVGEAAD
                     GAEAVKLAAHLKPDVVLVDLEMPCCDGYVCTQELTEHALCSAVVVLSIHSDAASRARA
                     RAAGAKLFLPKGTPMGPLVAAIQRAAGRDEAAPLPSRPGSRVRRTPAWPLPPAVAHPA
                     PPAGAINLGPASPKL"
     gene            complement(861..1451)
                     /locus_tag="VF276_19205"
     CDS             complement(861..1451)
                     /locus_tag="VF276_19205"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX6609037.1"
                     /translation="MSEMGFCMRCGTALREERPPGDADRRWVCTNPACGQIFYRNAKP
                     CAGALVEREGAVLLVRRNIEPFRDAWDIPGGYLHAWEHPVEATRRELREETGLEIEVT
                     RLLGIFIDRYGSNDANTFNVYYRARPVGGALHPADDAAEARWFGPGELPANLAFPGHI
                     PAVLRAWQASVAAEPHPEPATDGTLAVHVAAGVGPE"
     gene            complement(1471..2610)
                     /locus_tag="VF276_19210"
     CDS             complement(1471..2610)
                     /locus_tag="VF276_19210"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6609038.1"
                     /translation="MINAFLRREPGIAEAIARLGKYAYAPEVLSRLATYLRGLPAPQL
                     DHIPPTDLGHALGLDEQATLELLVAAVHEGLCDLYWTAHCAQCNKTAGEWAHLAGARH
                     ETDCAACGARLELTLDQNLHVRFALNRRYRTGRLGVPATSRAQPLITPVAGTVTGLDL
                     LNLQVFRDLLAGEVLLPDESLRVTRVALLFSDLRGSTALYARKGDPVAFGLVREHYGL
                     ITAQVRAARGAVVKMVGDGMMASFADPAAGLRASLAIQQDLARFNQRRGLRGDDALRL
                     KLGLHAGACLSVNLNGRMDYFGTAVNIAARVEGLATGDDVVTTAAVLAEAGARQVLDE
                     ARRAGAQVSEFATTLRGLEEAVQVVRITPRLAQAGRPAVPVATGA"
     gene            complement(2732..3157)
                     /locus_tag="VF276_19215"
     CDS             complement(2732..3157)
                     /locus_tag="VF276_19215"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX6609039.1"
                     /translation="MKKSALLRKVSAETGVPYSTVKKVMDSALEHIAGSLGTGERVVL
                     SGFGTFALRPYRARQWVNPQTGQQVDIPAALRPGFVTSAALKRRLAGAPHAPEAGGDS
                     GSDGAAASITARSRRAASSMRQVRTRKSGGQAVQASSGK"
     gene            3428..4006
                     /locus_tag="VF276_19220"
     CDS             3428..4006
                     /locus_tag="VF276_19220"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6609040.1"
                     /translation="MSYTAKRMIILLMMWGLIVFVGVTVRININTPSVPQVTPTPAAS
                     PTAAPVAAGADTVAALQQALARNPNDMTALAGLANIAYQNQDWTQAIDLYGRVLAQDP
                     HNVDVLVHLAAAQLYAVRFADAKQSLQQAVDLAPDRTDAHLLLGLALSRQSPPDMAGA
                     QAEWKRVIALAPGSDIAKQAQDLLDNGAGPGQ"
     gene            4015..4755
                     /locus_tag="VF276_19225"
     CDS             4015..4755
                     /locus_tag="VF276_19225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6609041.1"
                     /translation="MAITFIVIGGGCYGSHHLRRLEKGRARGTIGADDRILVVDRNPN
                     CQVRRELAAQPNPLVEVVRADWLEFMKSEFDHRPATDEVVPAPIATHLAAEWLLWSLE
                     SRLPAGGVRQVPFDDAFGGLPYEYLSPAGERFFSAANWICPPNCRAPRKCPAILAPRT
                     WDLEDTIRDFSRRHADRYAEPAIFRTAYRTTGIETIPVGFYRETRDRLLALAADGTAA
                     GKQVIVSTLSPCHGAANLLQFAAPVPSR"
     gene            4785..5330
                     /locus_tag="VF276_19230"
     CDS             4785..5330
                     /locus_tag="VF276_19230"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEX6609042.1"
                     /translation="MDVDQTAKLRTGLDDALSNLMAVLPGADNEPGWDVATDEEGWTV
                     RQVLAHLASAEASMNVLIDRALAAAREQRPVGDLIARGKDGSPFDLNLWNRRQVEKRA
                     TQPPSALRLELTEERARTLRTLKTHTAEELAAPAWHPALGETRVEGIYKIMGIHMRDH
                     TRAIKRALREGLHGRYWADVE"
     gene            5450..6067
                     /locus_tag="VF276_19235"
     CDS             5450..6067
                     /locus_tag="VF276_19235"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011231937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEX6609043.1"
                     /translation="MAYELPPLPYPFDALEPNIDAKTMEIHHDRHHAAYVNNLNKALE
                     GHPELGNQSVEDLIRNINQVPEDIRTAVRNNGGGHANHSFFWKIMGPNGGGEPTGAVA
                     QAINSTFGGFQAFKDKFNAAGAGRFGSGWAWLVADRNGNLSVTSTPNQDSPLMEGQTP
                     ILGCDVWEHAYYLKYQNKRPDYLAAWWNTVNWDEVNKHYQQARQG"
     gene            6149..6991
                     /locus_tag="VF276_19240"
     CDS             6149..6991
                     /locus_tag="VF276_19240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6609044.1"
                     /translation="MLRSPLSLPSARRTILAGGLLVLLAACDSGAAPATPVLPATATL
                     PAATATPGAPTAAPTATSAPATPTSGSNAAIYATYTALALNDAATATAAAAPAPPSPT
                     ALPPTPAPTVPAAVLGTYRLIITARAEGYRVYQQPGTLVISAPPPGSDHSAEVQLWTG
                     VRTGKEYADLSPGKTVGGLYVTTSAALLPASFPQDQSFAARVVYNPATKFLAVNPDHP
                     RPLPGGWSTGPNNKAGKPRPITAGTMLLDVGTRGQVSGQIDLHSQPAGGADLLYSGGI
                     QGEK"
BASE COUNT         1155 a         2563 c         2296 g         1112 t
ORIGIN      
        1 gggtcgatgt ggctccgggg accggttcgg gggtgtgcgg acggctagag ggccgcgccg
       61 gcacacgcgg ccccgaatat cggtgccaat ggcggcaaat atccgccttg agaccgatca
      121 ctactcagcc gaaattcgtt atagtatata caacgcgggc cagtaccgca ggtcgcccga
      181 ctttgttaca attgagtcag gttgggagta ccagtgccaa tccattggtg cgtgacatcc
      241 cgaccggcgg cctgtcgcaa ctttataaat ttcagcccgt ccccgcccat gcgcggctgg
      301 agagattgac gtgtagctat ggataaccag ggaaccacaa acgggaagat tcgtgtttta
      361 attgccgatg atgtagagtg catgcgcgaa agtttgcgca ccgtcttgag tctcgattcc
      421 tcgctcgaaa tcgtcggcga agcggccgat ggcgcggaag ccgtgaaact ggccgctcat
      481 ctcaagcccg atgtggtcct ggtcgatctg gaaatgccgt gctgcgacgg ctacgtctgc
      541 acccaagaac tcacggagca cgccctctgc tcagccgtgg tggtgctgag cattcatagc
      601 gatgccgcca gtcgcgcccg cgctcgtgcc gccggcgcga aattgttttt gcctaagggc
      661 acgcccatgg ggccgctcgt ggccgcgatc cagcgcgccg cgggccgcga tgaagccgcc
      721 ccgctgccgt cgcgtcccgg atctcgtgtc cgccgcacac ccgcctggcc cctgccgccc
      781 gccgtggcgc accccgcgcc ccccgccggc gcgatcaacc tggggcccgc ctcgcccaaa
      841 ctctgagctt tcgctagccg ctattccggt ccgacgcccg ccgccacatg gacggccagg
      901 gtgccgtctg ttgccggttc gggatggggc tcggcggcca cgctggcctg ccacgcgcgc
      961 agcaccgccg ggatgtgacc ggggaaagcc aggttggcgg gtagctcgcc gggtccgaac
     1021 cagcgcgcct ccgccgcgtc gtccgccggg tgcagcgcgc cgcccaccgg acgcgcccgg
     1081 tagtacacat tgaacgtgtt ggcgtcgttc gagccgtagc ggtcgataaa gatgcccagc
     1141 agccgggtta cttcgatctc cagcccggtt tcctcgcgca gttcgcggcg tgtggcctcc
     1201 accgggtgtt cccaggcgtg cagatagccg ccgggaatat cccaggcatc gcggaagggt
     1261 tcgatattgc gccggaccag caacacggcc ccctcgcgct cgactagcgc gcccgcgcac
     1321 ggtttggcgt tgcgatagaa gatctgtccg caggcggggt tggtgcaaac ccagcgcctg
     1381 tcggcgtcgc cgggcggccg ttcttcccgc agcgccgtcc cgcaccgcat gcaaaagccc
     1441 atctcgctca tcgggatctt tccccggcgg tcatgcgccg gtcgccacgg gcacagcggg
     1501 gcgcccggcc tgcgccaggc ggggcgtgat gcgcacgacc tggacggcct cctccagccc
     1561 gcgcagagtc gtggcgaact cgctcacctg ggcgccggcc cggcgcgctt cgtcgaggac
     1621 ctgccgcgcg cccgcttcgg ccagcacggc ggcggtcgtc accacatcgt cgcctgttgc
     1681 cagcccttcc acgcgcgccg cgatattcac cgccgtgccg aagtagtcca tgcgcccgtt
     1741 caggttgacg ctcaggcagg ccccggcatg cagccccagc ttgagtcgca gggcatcatc
     1801 gccccgtaag ccgcgccgct ggttgaagcg cgccaggtcc tgctggatgg cgaggctcgc
     1861 gcgcagcccg gcggccggat cggcaaacga ggccatcatg ccgtcgccca ccatcttgac
     1921 gaccgcgccg cgcgccgcgc gcacctgggc ggtgatcaag ccgtagtgct cgcgcaccag
     1981 tccgaaggcc accgggtcgc ccttgcgggc gtagagcgcc gtcgagccgc gcagatcgct
     2041 gaacagcagg gccacccgtg tgacccgcag gctctcgtcg ggcagcagca cttcgccggc
     2101 cagcagatcg cgaaagacct gcaggttcaa cagatcgagt cccgtcaccg tcccggccac
     2161 cggcgtgatc agcggctgcg cgcgcgaggt cgcgggcaca ccgaggcgcc ccgtccgata
     2221 gcgccggttg agcgcgaagc ggacgtggag gttctggtcg agagtcagtt cgagccgcgc
     2281 gccacacgcc gcgcagtcgg tttcgtgccg ggctccggcg aggtgtgccc actccccggc
     2341 ggttttgttg cactgggcgc aatgcgcggt ccagtagagg tcacacaggc cctcgtggac
     2401 ggccgcgacc agcagttcca gcgtggcctg ttcgtccagg cccagggcgt gccccagatc
     2461 ggtgggtggg atgtggtcca gttgcggggc cggcaggccg cgcaggtacg tggctaaccg
     2521 gctgaggact tcgggcgcat acgcatactt gcccaggcgg gcaatagcct ccgcgatgcc
     2581 cggctcgcgg cgcagaaacg cattgatcat gggaacctct ggcgggtggc agctcccggc
     2641 tagcgaatgg cgtgcccccg cggcgacgct gctgcgggga tcttatacgg tgtacgtatc
     2701 acgcccggcg ccagatgttc ccggcgccgc cctacttgcc gctgctggct tgcaccgcct
     2761 ggccgcctga tttgcgggtg cggacctggc gcatgctgct ggcggcgcgg cgagagcggg
     2821 ccgtgatcga ggccgcggcg ccgtcactac cgctgtcccc gcccgcttcc ggcgcatgag
     2881 gcgccccggc cagccggcgc ttcaaggccg cgctggtgac gaaaccgggg cgcaatgccg
     2941 ccggaatgtc cacctgctgg cccgtttgcg ggttcaccca ttggcgcgcc cggtatggcc
     3001 gcagggcgaa cgtgccgaac ccgctgagca ccactcgctc gcccgtcccg agtgagccgg
     3061 cgatgtgctc cagcgccgaa tccatcacct tcttgactgt cgagtagggt actcccgttt
     3121 ccgcactaac tttacgtagc agagcgctct ttttcatggg atccctcact ttcgatagtt
     3181 gctgctaatc gaacacctcc gcgcctgacg ggccaggggc ggcgtagacg agactggagg
     3241 agtcccggtt gctagcaagt atatagggca tacaagtgat tgtcaacagg gcaattgcac
     3301 tacccgatag atgatcgcaa aatcgaggta cagtagaccc tggccgccct atccggcgat
     3361 ctccagcatg agcttgcttg cccttagtat gcttgccgcg gaccccgctt tcgcggataa
     3421 tagacctatg agctacaccg ccaagcgcat gattattttg ctgatgatgt ggggtctgat
     3481 cgtctttgtc ggcgtgacgg tccgcattaa tataaatacg ccatcagtgc cgcaagttac
     3541 gccgaccccc gccgcgtcgc cgacggcggc gcccgtggcc gccggcgccg atactgtggc
     3601 cgcgctgcaa caggcgctgg cccgcaaccc caacgatatg accgccctgg ccgggctggc
     3661 gaatatcgcc taccaaaacc aggactggac gcaggccatc gatctctacg ggcgggtgct
     3721 ggctcaggac ccgcataacg tcgacgtgct ggtgcacctg gcggcggccc agctctatgc
     3781 cgtgcgcttt gccgacgcca agcagtcgct gcaacaggcg gtggacctgg cgcccgaccg
     3841 caccgacgcc catctgctgc tgggcctcgc gctgagccgc cagtcgccgc ccgacatggc
     3901 gggcgcccag gccgagtgga agcgcgtgat cgcgctggcg cccggcagcg acatcgcgaa
     3961 gcaagcccag gatctgctcg acaacggcgc cgggccgggc cagtaggaca cgctatggcg
     4021 atcacgttca ttgtcatcgg cggcgggtgc tacggcagcc atcatctgcg gcggctggaa
     4081 aagggccgcg cccgcggcac catcggcgcg gatgaccgca tcctggtcgt ggatcgcaac
     4141 cccaactgcc aggtgcggcg cgaactggcc gcgcaaccca acccgctggt cgaagtcgtg
     4201 cgcgccgact ggctggagtt catgaagtcc gagttcgatc accggcccgc caccgacgag
     4261 gtggtgcccg cgcccatcgc cacgcacctg gccgccgagt ggctgctgtg gagcctggag
     4321 agccgcctgc cggcgggcgg cgtgcgccag gtgccgttcg acgatgcgtt cggcggcctg
     4381 ccctacgaat acctcagccc ggcgggcgag cgcttcttca gcgccgcgaa ctggatctgt
     4441 ccgccgaact gccgcgcccc gcgcaagtgc ccggccatcc tggccccgcg cacctgggac
     4501 ctcgaagaca cgatccgcga cttcagccgc cggcacgccg accgctacgc tgagccggcg
     4561 atcttccgca ccgcgtaccg cacgaccggc atcgagacca tcccggtcgg cttttatcgc
     4621 gagacccgcg atcgcttgct ggccctggct gcggacggga cggccgccgg caagcaggtg
     4681 atcgtctcga cgctatcacc ctgccacggc gcggcgaacc tgctacagtt tgccgcgccg
     4741 gtgcccagcc ggtaacccgg ccccgcacac ggaggattct tcagatggat gtggatcaga
     4801 cagccaagct acgcaccggt ctggacgatg ccctctcgaa cctgatggcc gtcctgccgg
     4861 gggccgacaa cgaacccggt tgggacgtgg cgaccgacga ggagggctgg acggtgcgcc
     4921 aggtgctggc ccacctggcc tccgccgagg cgagcatgaa cgtgttgatc gatcgcgcgc
     4981 tggcggcggc ccgcgaacag cgccccgtcg gcgacctgat cgcccgcggc aaggatggct
     5041 cgcccttcga cttgaacctc tggaaccgcc gccaggtcga gaaacgtgcc acccagccgc
     5101 cctccgcgct gcgcctcgaa ctgaccgagg agcgggcccg gacgctgcgc accctgaaaa
     5161 cacacactgc ggaagaacta gccgcccccg cgtggcaccc cgcgctgggt gaaacgcgcg
     5221 tcgaaggcat ctacaagatc atgggcatcc atatgcgcga ccacacgcga gctatcaaac
     5281 gggcgctgcg cgagggtctg cacgggcgct actgggccga tgtcgaataa gctatgggat
     5341 cgattcttgc tggcactatg ttagctcttt aagagcggct tgtgattttg tgacccaggg
     5401 ccgggctacg gtgctgtacc ggcccactat ataaaaggat ggtgtaccca tggcttatga
     5461 acttcccccc ttaccatatc cctttgacgc gctggaaccc aatatcgacg ccaagacgat
     5521 ggagatccat cacgaccgcc accacgcggc ttacgtgaac aatctcaaca aggcgctcga
     5581 aggccacccc gaactgggca accagagcgt cgaagacctg atccgcaaca tcaaccaggt
     5641 gccggaggac atccgcaccg cggtccgcaa caacggtggc ggccacgcca accatagctt
     5701 cttctggaag atcatgggcc ccaacggcgg cggcgagccg accggcgcgg tggcgcaggc
     5761 catcaactcg accttcggcg gcttccaggc gttcaaggac aagttcaacg ccgcgggcgc
     5821 cgggcgcttc ggctccggtt gggcctggct ggtcgccgac cgcaacggca acctcagcgt
     5881 cacctcgacg ccgaaccagg acagcccgct catggaaggc cagacgccga tcctgggctg
     5941 cgacgtgtgg gagcacgcgt attatctgaa gtatcagaac aagcgccccg actatctagc
     6001 cgcctggtgg aacacggtga actgggacga agtcaacaag cattatcagc aggcccgcca
     6061 gggctaagca ggcggcccgc atgggcagcg gtccgggccg ggcgcccgga ccgcttcgtt
     6121 tacccgcgcc ccacaggaga ccccgccggt gttgcgttcc ccattgtcct tgccttccgc
     6181 ccggcggacc atcctggccg gggggctgct ggtgctgctg gccgcctgcg actccggcgc
     6241 ggcgcccgcc acgcccgtcc tgcccgccac ggcgaccctg cccgccgcta ccgccacgcc
     6301 gggcgcgccc acggcagcgc cgaccgcgac cagcgctccc gcgacgccca cttccggctc
     6361 gaacgctgcc atctacgcga cctacacggc cctggccctg aacgacgccg cgaccgcgac
     6421 ggctgccgcc gcgcccgccc cgccctcgcc gacggccctg ccgcccaccc ccgcgcccac
     6481 cgtgcccgcc gccgtgctcg gcacctaccg cctgatcatc acggcgcgcg ccgagggcta
     6541 tcgcgtctac cagcagcccg gcacgctcgt catcagcgcg ccgccgcccg gtagcgacca
     6601 ctccgcggag gtgcaactct ggaccggcgt gcgcaccggc aaggagtacg ccgacctcag
     6661 ccccggcaag accgtcggcg ggctctacgt gaccaccagc gcggccctgc tgcccgccag
     6721 cttcccccag gaccaatcct tcgccgcccg cgtcgtctac aacccggcca cgaagttcct
     6781 ggccgtcaac cccgaccacc cccgcccctt gcccggcggc tggtccaccg gccccaacaa
     6841 caaggccggc aagccccgcc ccatcaccgc aggcaccatg ttgctcgacg tgggcacgcg
     6901 cggccaggtc agcgggcaga tcgatctcca ttcccagcct gccggcggcg ccgatctgct
     6961 ctacagcggc ggcatccagg gcgagaagta gggcggggat gggccgggct tgctccgccg
     7021 gctgtcgagt gccggaaggc cgttgctcat ttccgcgccg tctgccggat caccggcggc
     7081 ggcccatagc gcgcgatgat ccaggcgcat gtcgcgtggg ctagca
//
LOCUS       DASUYC010001177         4814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1601725, whole genome shotgun sequence.
ACCESSION   DASUYC010001177 DASUYC010000000
VERSION     DASUYC010001177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4814
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1601725"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VF276_19245"
     CDS             complement(<1..690)
                     /locus_tag="VF276_19245"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX6609045.1"
                     /translation="MTYSQAPLEPSPDAGKHLLHGLNPAQQAAVQTVGGPILIIAGPG
                     SGKTRVLTHRIAYLVEAAHVDPYRICAVTFTNKAAKEMKHRLEALCGEDANKITVGTF
                     HALGVRMLRQDIDKIGYDRDFAIYDDEDQIGLVKQALKDLNFDEKQYAPRAVLSFISK
                     AKSALLEPAAAAEATENYFQEIAARVYKRYQELLARNRAVDFDDLIRLPIKLLVEDPA
                     VRDKWQRRFQHI"
     gene            1023..2315
                     /locus_tag="VF276_19250"
     CDS             1023..2315
                     /locus_tag="VF276_19250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6609046.1"
                     /translation="MEFMASDVSTPPIAPLVAASASGAKLAPPATEGRAWKLGFFAIA
                     ALMALFLLLRPVYYPYPPDHYVYLAHHLALGDLTVDNLPLDYGDYVNWNGHKYLPLGP
                     LPGIVLIPFLPLLDAGLQLAWIGHLFTLLSIWLLYRVLRRLQVPGEHRKWAILLSFGG
                     TVYYSGALMGTAWYFAHIVAITCLLVAISAALGGRRTWLVGLFLGLAAMTRLTMLFAL
                     PFFLWILWRGTGRAGVGVPPSSRFAALTLLSLGLAGPLVLLFGYNYARFGNPLESGYG
                     LAVMGGPVLGEALAYGLFSPVHIPKNLFMLLLQGPLPYPSADAPVLKFPYIQPTPWGM
                     GIFFTSPAFVCIFKARRSDPLVQACWLGVIFVMAPIITYYGIGWVQFGFRYALDFTLF
                     LLLLTVCGWRGSLSNLVRGLVLLSVLINLLGGIWLMLV"
     gene            2456..4240
                     /locus_tag="VF276_19255"
     CDS             2456..4240
                     /locus_tag="VF276_19255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX6609047.1"
                     /translation="MSTLQRPAVAAPRRHLALNSFWGSLAILAAAQVALALLVSPARD
                     YPVEDDWAYAQVVQSILQGHLQLSEFAGPSTVFHAAWGALWCAIFGFSFTTLTVANLV
                     MGVLAALAFWVLLRELGFSAWPSLIGALTLLLNPLTMFLSYTFMTDLTSLALLLAALA
                     AFAAACHRTPSLGYLLLSGLFAAAAVLTRQITFAAPLAIAVVWLRQWGFRRGVVGAAA
                     AVLPPVVAYGLLQAWVNTQGGPPWAWGLYSPGAVLAYVANPASLLIHVVRFLYIAGFL
                     TFLLLPIFALLLSALFRRGVGAPLAQVRRAVALRAVHWPTFRYITLGVAVAAIYAAFY
                     FVGGSFNGGSDILQRRGYWPTLSGLTDKLPVFPLPFWIALTIVIVPLVAALLVLLGHG
                     LVRALQRGPRDPALLGVTLSMLVILGLTMSVTAYHDSLSLPLLAGLIIFLLARVRGRV
                     APVGPAVVVLVLFATFGILSLRDLHNWHTAAWRAGQALLAAGVPLTHISGGNEWNYWY
                     LGEEEVRQARAAGRPMSFGVKLIQPEYVISFSPQITWIVDAPFPASGPPYRVCAQLPY
                     ERPLHAAPAYVYVLTDGGCPLSGLPQAP"
     gene            4492..>4814
                     /locus_tag="VF276_19260"
     CDS             4492..>4814
                     /locus_tag="VF276_19260"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX6609048.1"
                     /translation="MNNLKPLIPYLRTHQGAILLGVLTSLIGTILALLQPYLIGSAID
                     ALQHSRPVSDILQLAALILGFAAVQGVFTFFTRYSINKVSREVEYELRRDYFAHLERM
                     PQTFF"
BASE COUNT          685 a         1466 c         1532 g         1131 t
ORIGIN      
        1 gatgtgctgg aagcggcgct gccacttgtc gcggacggcg gggtcctcta ccagcagttt
       61 gatcggcagg cggatcaggt cgtcgaagtc gacggcgcgg ttgcgcgcga gcagttcctg
      121 gtagcgcttg tagacgcggg cggcgatctc ctgaaaatag ttctcggtgg cctcggccgc
      181 ggcggcgggt tccagcaggg cgctcttggc tttgctgatg aaggacagca cggcgcgcgg
      241 cgcgtactgc ttctcgtcga agttcaggtc cttcagcgcc tgcttgacca gcccgatctg
      301 atcctcgtcg tcgtagatgg cgaagtcgcg gtcgtagccg atcttgtcga tgtcctggcg
      361 cagcatgcgc acgccgagcg cgtggaaggt gccgacggtg atcttgttcg cgtcctcgcc
      421 gcagagcgcc tccaggcggt gcttcatctc cttggcggcc ttgttggtga aggtgacggc
      481 gcagatgcgg tagggatcga cgtgggcggc ttccaccagg taggcgatgc ggtgggtgag
      541 cacgcgggtc ttgccgctgc cggggccggc gatgatcagg atcgggccgc cgaccgtctg
      601 cacggcggcc tgctgagccg ggttcaggcc gtgcagcagg tgcttgccgg cgtccgggct
      661 cggctccaaa ggggcttggg aataggtcac aggggagggg cctccgcttc cgataagatc
      721 gggcatatcc cgtattttac ccgatctacg gggatgcttg ccagggtcgt gtatggtgga
      781 ccagttcagg gaccaacggg cccgccaagt gaagacctct agtccttccg caactcgtgg
      841 ccgatatgct aagctgcatg catttaccgg tactgaccgg caacacagct ttgtgactct
      901 gtcggtaagg gcattaagta gacgggacca tatgctactc ccgcactgta aatcacgcaa
      961 ccaagatcga gtgtcgatta tcggcgccac ggcgcattgc aaagagggga ttatctgggg
     1021 gtatggaatt tatggcaagt gatgtgagta cgccgcctat tgcccccttg gtagcggcta
     1081 gcgccagcgg ggcaaagctc gcgccgccgg caaccgaggg ccgggcatgg aagctcggtt
     1141 tcttcgccat cgccgctctc atggcgttgt tcctgcttct ccggccggtc tactacccct
     1201 acccgccgga tcactatgtg tacctggcgc atcatctcgc ccttggagat ctgacggttg
     1261 acaacctccc gctggattat ggcgactatg tcaactggaa cgggcataag tacttgccgc
     1321 ttggaccact ccccggcatc gtgctgattc cgttcctgcc gttgctggac gccggactgc
     1381 aattggcctg gatcggccat ctgtttacgc tgctcagtat ctggctgctg taccgtgtgt
     1441 tacgccggtt gcaggttccc ggtgagcatc ggaaatgggc gatcctgctc tcctttggcg
     1501 gcaccgtcta ttactccggc gcgctgatgg gcaccgcctg gtacttcgcg catattgtag
     1561 cgatcacgtg cttactcgtg gctatttcag cagcgttagg cgggcgccgc acctggctgg
     1621 ttggcctgtt tctggggctg gccgccatga cccgcctgac catgttattt gcgctaccct
     1681 tttttctatg gattctctgg cgcgggaccg gtcgcgcggg tgttggagtc ccgccttctt
     1741 cccgttttgc cgccttgacc ctcctcagcc tggggttggc ggggccgctc gtattgttgt
     1801 tcggatataa ttacgcgcgt ttcggcaacc cgctagagag cggctatgga ctggcggtaa
     1861 tgggtggtcc ggtattgggc gaggcactcg cttatggcct gttcagcccg gtacatattc
     1921 caaaaaacct gtttatgttg ctgttgcagg ggccgctacc ttatccatcc gccgatgctc
     1981 ccgtgttaaa gtttccctat attcagccta ccccgtgggg tatgggtata tttttcacct
     2041 ccccggcctt tgtgtgcatt ttcaaagccc ggcgtagcga tccgctggtg caggcttgct
     2101 ggctaggcgt catctttgtg atggcaccga tcatcaccta ctacggcatt ggctgggtgc
     2161 aattcggatt tcggtatgcg cttgacttca cgctattcct cctgttactg acggtgtgcg
     2221 gctggcgggg atcactcagc aacctggttc gggggctggt tttgctcagc gtgctgatca
     2281 atttattggg gggaatctgg ttaatgctcg tgtaatcgcc caagtcatcc gactccccgt
     2341 gcttcctgag cttgcggcct ttgcccggcc ggtggcgccc ccgattctcc gtatggctgc
     2401 tggagcggat agttgatata atagcgtgat attcgctgct caaaggatga tgcacgtgtc
     2461 cacgctccag aggccggcag ttgccgcgcc gcgccgccat cttgcactca actccttctg
     2521 ggggtcgctg gcgattcttg cggcggcaca ggtagccctg gctctcctgg tctccccggc
     2581 gcgcgactac ccggtggaag acgactgggc ctacgcgcaa gtcgtacaaa gcatcctcca
     2641 gggccacctt cagctgtcag agttcgccgg cccaagcacc gtctttcacg cggcctgggg
     2701 cgcgctctgg tgcgcgatct tcggcttctc gttcacgacg ctgacggtcg ccaacctggt
     2761 gatgggcgtc ctggcggccc tggcgttttg ggtgctgcta cgcgagcttg gcttctcggc
     2821 gtggccgagc ctgatcggcg cgctgactct gctgctcaat ccgctgacca tgttcctcag
     2881 ctataccttc atgaccgatc tcacctcgct cgcgctgttg ctggcggcgc tggcggcctt
     2941 tgccgccgcc tgtcaccgaa caccgtccct gggctacctg ctattgagcg ggttattcgc
     3001 tgccgcggcg gtgctgaccc gccagatcac gtttgccgcg cccctcgcca ttgccgtggt
     3061 gtggctccgg caatgggggt tccggcgggg cgtggtcggg gcggcggctg ccgtattgcc
     3121 gccggtcgtc gcctatgggt tgctgcaggc ctgggtgaat acgcaaggcg gcccgccctg
     3181 ggcctggggc ctgtatagcc ccggcgcggt gctcgcatat gtcgccaatc cggcctccct
     3241 gctgatccac gtggtgcgct tcctctatat cgccggcttc ttgaccttcc tgctcctgcc
     3301 catcttcgcg ttgctgctaa gcgcgttatt ccggcgcggg gtgggcgcgc cgttggccca
     3361 ggtccggcgc gcggtcgctt tgcgtgcggt gcactggcct accttccgct atattaccct
     3421 gggagtggcg gtggcggcga tttacgccgc gttctatttt gttgggggtt cgtttaacgg
     3481 cggctcggat attctccagc ggcgtggcta ctggccgaca ttgagcggcc tcacggataa
     3541 gctgccggtg tttccgctgc ccttctggat agccttgacg atcgtgatcg tcccactggt
     3601 cgcggcactg cttgtcctgc tggggcatgg gctggtgcgc gcgttgcagc ggggtccgcg
     3661 cgacccggcc ctgctgggcg taaccctctc tatgctggtt atcctcggtc tcaccatgag
     3721 tgtgacggct tatcacgaca gtctctcgct gccgctcctg gccggtctga ttatcttttt
     3781 gctggcgcgg gtgcgtggcc gcgtggcgcc ggtggggccg gccgtcgttg tgctggtgct
     3841 gttcgctact ttcggcatcc tcagcctgcg cgatctgcac aattggcaca cggccgcgtg
     3901 gcgggccggc caggccctgc tggcggccgg cgtgccgctc acgcatatca gcggtggtaa
     3961 cgaatggaac tactggtatc taggggaaga agaagtgcgc caggcgcggg ccgccgggcg
     4021 gcccatgagt tttggcgtga agctgatcca gccggagtat gtgattagct ttagcccgca
     4081 aattacctgg atcgtcgatg cgcccttccc cgcatccggc ccgccatacc gtgtctgcgc
     4141 gcaactcccc tatgagcgac ctttacacgc cgcccccgcc tacgtttacg tgctgactga
     4201 tggcggctgc ccgctcagcg ggctaccgca agccccgtaa cccgataggc tttcaccctg
     4261 gaggcctggc gttaggatcg ggaaggctcg tcggctgcac ttgcccggtg tgttaccggc
     4321 ggcgctgccg gcaatgcgtc aggtctccgt cgggccggcg aattgggccg tttggcggat
     4381 gtcgggttac aacctgcgac cgggcatggc agttgcttcg tacgttatta ttgaagggca
     4441 gtgcgccggg atgtgacccg gctttccgca tttgtgcgca ggagatccgc gttgaataac
     4501 ctgaaacctc tcattcccta cttgcgcacg catcaagggg ctatcctcct gggcgtgctc
     4561 acgtcgctga tcgggaccat tctggccctg ttgcagccct atctgatcgg cagcgcgatt
     4621 gatgcgcttc agcacagccg gccggtgagc gatattttgc aactggccgc gctgatcctc
     4681 gggttcgcgg cggtgcaagg tgtgttcacc ttcttcaccc gctacagcat caacaaagta
     4741 tcgcgcgaag tcgagtatga actgcggcgc gattacttcg cgcacctgga gcgcatgccg
     4801 cagacctttt tcca
//
LOCUS       DASUYC010001178         2597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_807803,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001178 DASUYC010000000
VERSION     DASUYC010001178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_807803"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VF276_19265"
     CDS             <1..536
                     /locus_tag="VF276_19265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6609049.1"
                     /translation="SAPLISSGRTRSLTMVVGSQGPAACTTAGSWSSGRNVLPRKLAG
                     SSASIERRLRRALADHHVSYASCYRPLLAPVLDWATLLREDRQVVLAGADSSHTARIH
                     LLRVSLPYWGGSLPLAWARWPQHVAQAPGQYWDQLDTLFAQVAARLPAGLEVVVTADR
                     ACAVPNFIARVAAQGWH"
     gene            655..1239
                     /locus_tag="VF276_19270"
     CDS             655..1239
                     /locus_tag="VF276_19270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6609050.1"
                     /translation="MCKDAGWRGASGVGLWGRGAAESLVVLTDLPARGAVLARYERRY
                     WIEPGFRNDKTRGWQGEASQVRGQAAQERRRVALAWARVVVLSVGLTAAREQLAWERQ
                     RRAAGARGQVRRAPAARRAAVGYGTARPALPWGLGDRAGPSWGQRWHRVQAQALILPP
                     LGPPGAAGRAGLDPILEVLALLARPRPNECSVRA"
     gene            1689..>2597
                     /locus_tag="VF276_19275"
     CDS             1689..>2597
                     /locus_tag="VF276_19275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6609051.1"
                     /translation="MSRKRIWFLVLLIITLVLPACGDVAATPTAVSSNPSGGGGTTEA
                     TATSAPSGGNTSSGGTAGTLNGVTLPADAAPPDQQVYITHYDNTADFTTVDFYESVYK
                     RGGAVADVLSDPLVRLDKNFKVTPAAATKWEVDSTGLVWTFHLDPNLIWTDDTPVTAD
                     DYVATLRYGADPKHAWDFTWYFQGVIKNWSEAVEGKVPLDQLGVTAADPHTLQITTVS
                     PAPYLPAMLLYSSALQKKALEAHGGLYNSDPATSVSSGPFVLKEWRKGDRLVYEANPK
                     YKGSNKPFIQKVIVIGAAPATDFASYQ"
BASE COUNT          371 a          887 c          962 g          377 t
ORIGIN      
        1 gcagcgcgcc gctgatcagc agcgggcgca ccaggtcgct caccatggtc gtgggcagcc
       61 aggggccggc ggcctgcacc acggcgggca gttggtctag cggcaggaac gtgttgccca
      121 ggaagctcgc cggcagcagc gcgagtatcg agcgccggtt gcgccgcgcc ctggccgatc
      181 accacgtaag ctatgccagt tgctatcgcc ccctgctggc cccggtgctc gattgggcca
      241 ccctcttgcg ggaggaccgc caggtggtgc tggccggcgc cgacagtagc cacaccgcgc
      301 gcatccacct gttacgggtc agtctgccct attggggcgg cagcctgccg ctggcctggg
      361 cgcggtggcc gcagcatgtc gcccaggcac cgggccaata ctgggaccaa ctcgacaccc
      421 tgttcgcgca ggtggccgcc cggctgccgg cgggcctaga ggtggtcgtg acggccgatc
      481 gcgcctgcgc cgtgcccaac ttcattgccc gcgtggccgc ccagggctgg cactgagtgg
      541 tgcgcgtgac cacgccaggc agccaccgct tccgcgctgc gcgcgggcag gcgcaggagg
      601 tgcgggtgct ggtggggcgg cacgtggggc cggcgctgga aggcgcgcgg ggcggtgtgc
      661 aaggacgcgg gctggcgggg cgcgagcggg gtcgggctgt gggggcgcgg ggcggcggag
      721 agtctggtgg tgctgaccga cctgccggcg cgcggggcgg tgctggcgcg gtatgagcgg
      781 cgctattgga ttgaacccgg atttcgcaat gataagacgc gcggctggca gggggaagcc
      841 agccaggtgc gggggcaggc cgcgcaggag cggcggcgag tggcgctggc gtgggcgcgt
      901 gtggtggtgt tgagtgtggg gctgacggcg gcgcgggagc agctggcgtg ggagcggcaa
      961 cggcgggccg ccggggcgcg ggggcaggtg cggcgggcgc cggcggcgcg ccgggcggcg
     1021 gtggggtatg ggacggcgag gccggcgtta ccgtgggggt taggggaccg ggccgggccg
     1081 agttgggggc agcggtggca ccgggtgcaa gcgcaggcgt tgattttgcc gccgctggga
     1141 ccgcccggtg cggcggggcg ggcggggctg gatccgatat tggaggtgct ggcgctgctg
     1201 gcccgcccgc gcccgaacga atgttctgtc cgcgcctgaa ccgccgcggg aaggggcgtg
     1261 attttctctc tcgctggcga ttcccccttc ccgtggcggg aaggggggta ggggggtgag
     1321 gttgttcgcg cgcccggatg ccgccgggcg tggtgcttga caagcggggc gggccggtgt
     1381 atcatcttcg cgccaagggg ggcggctctt cggctatttt gcatccagca ccggcacgcc
     1441 ggcaccggcc cgcgcgggat tcccttgcac cccacgccgg gaaccaccgg ggcgcggcgc
     1501 cggccccacc ccgttgcggc gcagtttccc ccatgggatc ggacgacggc ccaccacaca
     1561 cggcacaagg aggtgattac cccgccccgg caccggcgcc ggccaggcag cgctcacgcc
     1621 tgcggccccg cccaccctgt cgattgactg tgaaggctat cgggtttgac cgaacaggag
     1681 gccaacctat gagtcgcaaa cgcatctggt tcctcgtcct gctcatcatc accctcgtgc
     1741 tgccggcctg tggggatgtc gccgccacgc ccaccgccgt gagcagcaac cccagtggcg
     1801 ggggcggtac caccgaggcc acggccacca gcgccccgtc cggcggcaac acgtccagcg
     1861 gcggcacagc cggcaccctg aacggcgtga cgctgcccgc cgacgccgcc ccgcccgacc
     1921 agcaagtcta catcacccat tacgacaaca ccgccgactt taccaccgtc gatttctacg
     1981 agtcggtcta caagcgcggc ggcgcggttg ccgacgtgct ctccgatccg ctggtgcggt
     2041 tggataagaa cttcaaggtc acccccgcgg cagccaccaa gtgggaagtc gactccaccg
     2101 gcctcgtctg gacgttccac ctcgacccca acctgatctg gaccgacgat acgcccgtca
     2161 cggcggatga ttatgtcgcc accctgcgct acggcgccga ccccaagcac gcctgggact
     2221 tcacctggta cttccagggc gtgatcaaga actggagcga agcggtcgag ggcaaggtgc
     2281 cgctcgacca gctcggcgtc accgccgccg acccgcacac gttgcagatc accacggtgt
     2341 cgcccgcgcc ctacctgccc gcgatgctgc tctacagcag cgccctgcag aagaaggcgc
     2401 tggaggccca cggcgggctc tacaactccg accccgccac ctccgtctcg tccggcccct
     2461 tcgtgctgaa ggagtggcgc aagggcgacc ggctcgtcta tgaggccaac cctaagtaca
     2521 agggttccaa caagccgttc atccagaagg tgatcgtcat cggcgcggcc ccggccaccg
     2581 actttgcctc ctatcag
//
LOCUS       DASUYC010001179         2238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_376116,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001179 DASUYC010000000
VERSION     DASUYC010001179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376116"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VF276_19280"
     CDS             complement(<1..275)
                     /locus_tag="VF276_19280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6609052.1"
                     /translation="MRARRPPLAEIDTQALWQAIRAGRRREVLPWLLLLAGFCAVLAG
                     LASFFWLTTRDTPIDRIPLLGALLAFCVFLYRRLSAQRGFALATLAV"
     gene            complement(283..1263)
                     /gene="meaB"
                     /locus_tag="VF276_19285"
     CDS             complement(283..1263)
                     /gene="meaB"
                     /locus_tag="VF276_19285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006692993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEX6609053.1"
                     /translation="MTGPTTGAAAAPPAPAGLAARILAGDRLALSRAITRVENRRPEA
                     AELLRAITPHTGRAYLLGITGPPGGGKSTLTTQIVKLLRARGETVGVIAVDPTSALSG
                     GALLGDRIRMLEFHADPGVFIRSMASRGVLGGLAATTGDVARVLDAFGFDWVIVETVG
                     VGQGEVAVAALADTTLLIEVPGLGDDVQAIKAGVLEVADILVVNKADREGASRLATTL
                     RGMLSLAHTEGWKPPIVQTVAAEGKGVPELLDEVAKHRAYLEASGRRAERTRARLRAE
                     VLDRAQELVTARLIQLMDGDSEFAAALAGLERRELDPATVAADLAARLTG"
     gene            complement(1260..1685)
                     /locus_tag="VF276_19290"
     CDS             complement(1260..1685)
                     /locus_tag="VF276_19290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEX6609054.1"
                     /translation="MVTATERKIRVLVAKPGLDGHDRGAKVIARALRDAGMEVIYTGL
                     HQTPEMIAEAALQEDVDAVGLSILSGAHMALFPRVLEELRKRGMDDVLVCAGGIIPPD
                     DVEPVRQMGIKGVFGPGTSMEDVVRFIRANVRPEAEAGA"
     gene            complement(1864..>2238)
                     /locus_tag="VF276_19295"
     CDS             complement(1864..>2238)
                     /locus_tag="VF276_19295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6609055.1"
                     /translation="PTQVAFWQGTIQPADNHASGKAGDGQYHWINGWTGIINHRQLGV
                     VAGSLVDRSGSGTPELGILRVDGEDPSLNGLYKPPVQSGPLRITAVKGSCLTIASTGN
                     TTYQFDAAAHTWSCSSSAAVTP"
BASE COUNT          364 a          766 c          757 g          351 t
ORIGIN      
        1 aagacggcca gagtcgccag ggcgaagccg cgctgggccg acagccggcg atagaggaag
       61 acacaaaagg ccagcaacgc gcccagcagc gggatgcggt cgatgggggt gtcgcgggtg
      121 gtcagccaga aaaacgacgc cagcccggcc agcacggcgc aaaagccggc cagcaacagc
      181 agccagggca gcacctcccg ccggcgcccg gcccggatgg cctgccagag cgcctgggtg
      241 tcgatttccg cgagcggcgg gcggcgggca cgcatcgcag ggtcagccgg tcagccgggc
      301 ggccaggtcg gcggccacgg tggcggggtc gagttcgcgc cgctccaggc cggccagcgc
      361 ggcggcaaac tcggagtcgc cgtccataag ctggatcagg cgggccgtca ccagttcctg
      421 cgcccgatcg agcacctcgg cgcgcagtcg cgcccgcgtg cgctcggcgc gccgcccgct
      481 ggcctccagg taggcgcgat gcttggcgac ctcgtccagc agctcgggca cgcccttgcc
      541 ctcggcggcg acggtctgca cgatgggcgg cttccagccc tcggtatggg ccaggctgag
      601 catgccacgt agcgtggttg ccaggcggga cgcgccttcc cggtcggcct tgttcaccac
      661 caggatgtcg gccacttcca gcaccccggc cttgatcgcc tgcacgtcgt cgcccaggcc
      721 gggcacttcg atcagcagcg tggtatcggc cagcgctgct acggccacct cgccctggcc
      781 cacgccgacc gtctcgacga tgacccaatc gaagccgaag gcgtcgagta cccgcgctac
      841 gtcgccggtg gtggcggcga gcccgcccag cacgccccgg ctcgccatgc tgcggatgaa
      901 cacgcccgga tcggcgtgga actccagcat gcggatgcgg tcgccgagca gcgccccgcc
      961 gctaagggcc gaggtgggat cgacggcgat cacgcccacg gtctcgccgc gcgcgcgcaa
     1021 cagcttgacg atttgcgtag tcagggtgga tttgccgccg cccggcggcc cggtgatgcc
     1081 gagcaggtag gcgcgcccgg tatgcggggt gatggcgcgc agcagttccg cggcttccgg
     1141 gcggcggttc tccacgcggg tgatggcccg gctcagcgcc aaccggtcac ccgccaggat
     1201 acgcgcggcc aggcccgcgg gggccggagg ggccgccgcg gcgccggtgg tcggtccggt
     1261 catgcgccag cctcggcctc gggccgcacg ttggcgcgga taaagcggac cacgtcttcc
     1321 atgctggtgc ccggcccgaa cacgcccttg atgcccatct ggcgtaccgg ctccacgtcg
     1381 tcgggcggga tgatgccgcc cgcgcagacc agcacgtcgt ccatgccgcg cttgcgtagc
     1441 tcctccagca cgcgcgggaa gagcgccatg tgcgccccgc tgaggatgga gaggcccacg
     1501 gcgtccacgt cttcttgcag cgcggcctcg gcgatcatct ccggcgtctg gtgcagcccg
     1561 gtgtagatca cttccatccc cgcgtcgcgc agggcgcggg cgatgacctt ggcgccccgg
     1621 tcgtgcccgt cgaggcccgg cttggcgacc agcacgcgaa ttttgcgctc cgtagcagta
     1681 accatagtgc gttagcccct ttgctgaacg actaatgtgc cgcggcaaca aacatggctg
     1741 ccgctacgta atcataaccg aacggcccgg ctgcgacaag ccgtgggccg tgtggctaag
     1801 acttataaca aagggactgg attgctccag tcccaaggct cttgctttct aacttatctt
     1861 cgattacggg gttacagcag cggatgagct acaggaccat gtatgtgctg ctgcatcaaa
     1921 ctggtaagtt gtgttaccgg tggaagcgat ggtaaggcaa ctgcccttta ctgcggtgat
     1981 gcgtaaaggc ccggactgga cgggcggttt gtatagccca ttcaagctgg gatcctcacc
     2041 gtccacgcgc aggatgccca attccggtgt gcctgaaccc gaacgatcca ccagcgaacc
     2101 ggctactacc cccagttggc gatggtttat gatgcccgtc caaccattga tccaatggta
     2161 ctgcccatcg ccagccttac ccgaagcgtg attgtcggcc ggctgaatgg tgccttgcca
     2221 aaaggctact tgagtcgg
//
LOCUS       DASUYC010001180         5306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1243954, whole genome shotgun sequence.
ACCESSION   DASUYC010001180 DASUYC010000000
VERSION     DASUYC010001180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 5306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5306
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243954"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            546..1022
                     /locus_tag="VF276_19300"
     CDS             546..1022
                     /locus_tag="VF276_19300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6609056.1"
                     /translation="MRGQHSSRLSRTLTFALLLLGIALLSLPGTHALAASRVPGISIT
                     PVPVTTTGLQFTPALVTGGLSAGSLATYRLNLHNYGPESVPFKLDVINPDGWKVQVKT
                     SDVAIKAGGDVTVAVTVGAPVIKTGSKTAITVSAYSPTSSTKTQLNLQVLYPSTGP"
     gene            complement(1115..1519)
                     /locus_tag="VF276_19305"
     CDS             complement(1115..1519)
                     /locus_tag="VF276_19305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6609057.1"
                     /translation="MESLDFTFIVITIVMTVGAAGIAAALYRRYVPAAKARIRVVAIY
                     WSAGGNLLDVRYQVTRPGRVLPLDEIAILGADGRRAGTVAQVTRIGPLASRSALRRTG
                     GFVLFRNASGVARGDRVIAVVGRGQQEAFTVT"
     gene            complement(1583..2911)
                     /locus_tag="VF276_19310"
     CDS             complement(1583..2911)
                     /locus_tag="VF276_19310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003487680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX6609058.1"
                     /translation="MRTPKSATPGGPALDRRSFLKLAGGLAGGLAAGVVLPQSARAIP
                     VTGPAHADLSAPDREIPARTRHFYIYATDGYLTLPDQTRTYIRGFAGNNPPTTPVADP
                     ASLKGKAQLPAPIIEAVAGDDCYLHFRDISNANPNAPVDPHTVHLHGIHTSTQNDGFP
                     EGSFSVMDREVIYYFKPEHAGTFMYHCHVEASEHVQMGMYGALIIRPAANMGNTVYGG
                     VRHQGQLIDRFDKEYIMLLSDLDTRWHRMIEGHNIAFNPVDYRPDYWLLNGRCFPDTI
                     LPAQAVFAGNTGGPPPGYDSYVHVRTGQRLLLRMINMGYQTIPWHVHGYHFNIVGKDA
                     SPLVELGANGGLHDNYTVTIGSGETYDLIVEVMDKSGIYKNKSPQDVPYVPVPTADGG
                     FYTYDGTAATFFPQFFPMHNHDDYKVTNNGVYPGGALALMQADAPAGWRP"
     gene            complement(3422..4666)
                     /locus_tag="VF276_19315"
     CDS             complement(3422..4666)
                     /locus_tag="VF276_19315"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6609059.1"
                     /translation="MADHRSLLGRVGRLPGLLLLGLMLIGGAACSEAAAPPALPVETT
                     PVAAPPPTAPKGAPKGAPKEASKEAPPPRTTPTAIPTITPAGPAPATLAAGYQTRGRT
                     YYAQGAYDRALAEYNKALALQPQDADLYVDRAAAYAGLGQYDHAVADYTSAIKLHPND
                     AGAYNGRGEALAAQGALDRALADYNQAIQLQPDLAVAYTNRGNLYRRRGIVDRALADF
                     EQALHYNPRAAGAYLGRGDIYASQGDATRAIAEYNQALQVSPLLPDAYLGRADAYLAQ
                     GNYDRAVSDYSRALGIAPANAAGYLGRAHAYVAQGDDARALADYTQALALDANNAGAF
                     NGRGLIYARRGDYPHALADYTQAIRLQPDNATFYFNRALSYRQSGNRDAALADLQKVV
                     LLTNDLALRGQVEDQLRALLAK"
     gene            complement(4899..>5306)
                     /locus_tag="VF276_19320"
     CDS             complement(4899..>5306)
                     /locus_tag="VF276_19320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194383.1"
                     /note="catalyzes the interconversion of 2-phosphoglycerate
                     and 3-phosphoglycerate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEX6609060.1"
                     /translation="YDFIVVNYANGDMVGHSGKLSAAIKAVETVDTCLGRVVPAILAQ
                     GGVALITADHGNCEQLEDYETGEPHTYHTLNPVPFILVAPEGSPLRQVRLRPGRLCDV
                     TPTILDIMHLHPAPEMNCGTLIVGGAPQEESAS"
BASE COUNT          943 a         1652 c         1754 g          957 t
ORIGIN      
        1 accgtggccg ccggtgtgcc taacgcgctg ccgggcagga tcagactcaa cgtcatgatc
       61 gccaacagca cacgcatact ccaatggcgt tgctttatca tgcttaccac tcctcctcta
      121 tgcagatctt tgtcttcggg cgcgccctca gttcccgccg cgccgtgccg gtcggggccg
      181 aaccgggcgg cgacaacgcc ggtagttgtc cgggaatcgc ggaagaacca gtcctagccg
      241 aaaagtacta ggacttaaca ttcaagtata ccggaaatac taccaaaaac aacagtgccg
      301 gggccatcct tgcgaggttg taaccaaaat cctccgtgcg ccggccccgc gccgaccaca
      361 catgccgccc aaccgggcgt ctttgccgct ccgcgccttt ccaaaaccaa tttgcgacag
      421 gccgctactt tttggccggc cccgccgttg ctcgttacgt aagccactgc agaggcccga
      481 ttagcttagc taagctgaac attgttccat cttgcatcat aattctgccc aggaggctaa
      541 ctaccatgcg cggccaacac tcttctcgcc tgtcccggac actcacattc gcgttactgc
      601 tactcggcat tgctcttctc agcctgccgg gcacccatgc cctggcggcc tcgcgggtgc
      661 ctggtatttc gattacgccg gttcccgtga ccacgacggg cctgcaattc accccggccc
      721 tggtaaccgg tgggctgagc gccggcagcc tggccaccta ccgcctgaac ctgcataact
      781 acggccccga aagcgtgccg ttcaagctgg atgtgatcaa tccggatggg tggaaggtac
      841 aggtcaagac gagcgacgtg gcgatcaagg ccgggggcga cgtgacggtg gcggtgacgg
      901 tgggcgcacc ggtgatcaaa accggcagca agaccgcgat taccgtgagc gcctacagcc
      961 cgacgagcag taccaaaacg caactcaacc tgcaagtcct gtacccgagc accgggccgt
     1021 agcggaccgc gcaatgcgcg aacacagcga gggggtagcg cgacgctacc ccctcatctt
     1081 gcatacatgg ccctggcgcc cgcgggcggg atcgttacgt cacggtgaat gcttcttgtt
     1141 gtccacggcc gacgaccgcg atgacccggt cgccgcgcgc cacgccgctt gcattgcgga
     1201 agagaacaaa gcccccggtg cgccgcaacg cgctgcggct agccagcggg ccgatgcggg
     1261 tgacctgcgc cacggtgccg gccctccggc catctgcgcc tagaatggcg atctcgtcca
     1321 gcggcagcac ccggcctggc cgggtcacct ggtagcgcac gtccaggaga ttgccgcccg
     1381 cgctccagta gatcgcgaca acgcgaatcc gcgctttcgc ggcaggcacg tagcgccggt
     1441 acagcgccgc tgcgatgccg gcagccccta cagtcatcac gatcgtgatg acgatgaagg
     1501 taaaatcgag gctctccatc ggtgtttcct gttcccgccc ctggcgctta tcaacgacag
     1561 cccgcgcggt ggctccccca acctacgggc gccaacccgc cggcgcatca gcctgcatca
     1621 gcgccagggc cccacccgga tacacgccgt tgttggtcac tttgtagtcg tcgtgattgt
     1681 gcatggggaa gaactgcggg aagaacgtgg cggccgtgcc gtcataggtg tagaatccgc
     1741 cgtccgcagt cggtaccggc acatagggca cgtcctgcgg cgacttgttc ttataaatac
     1801 ccgacttgtc catcacttcg acgatcaaat cgtaggtttc cccggagccg atggtgacgg
     1861 tgtagttatc atgcaagccg ccgttggcgc ccaactccac tagcgggctg gcatctttgc
     1921 ccacgatatt gaagtgatag ccgtggacat gccaggggat cgtctgatag cccatattga
     1981 tcatgcgcag caaaagccgt tgcccggtgc gcacatggac gtaggagtca tagccgggcg
     2041 gcgggccgcc ggtattgccg gcaaacacgg cctgggccgg caggatggta tcggggaaac
     2101 agcgcccgtt gagcagccag taatcgggcc gataatccac cgggttgaac gcgatgttgt
     2161 ggccttcgat catgcggtgc cagcgggtat cgaggtcgga gagcaacatg atatattcct
     2221 tgtcaaaccg gtcgatcagt tgcccctggt gccggacccc accgtatacc gtgttgccca
     2281 tgttcgccgc cgggcggatg ataagcgccc cgtacatgcc catctggacg tgttcggatg
     2341 cttcgacgtg gcaatggtac ataaacgtac cggcatgttc gggtttaaaa tagtagataa
     2401 cctcccggtc catcacgctg aaagaccctt cgggaaagcc gtcgttttgc gtcgaggtgt
     2461 gaatcccgtg caggtggacc gtgtgggggt ccaccggcgc gttcgggttc gcgttgctga
     2521 tgtcgcggaa atgcaggtag caatcgtcgc ccgcgacggc ctcgataatg ggggccggca
     2581 gttgggcttt gcctttgagc gaggccgggt cggcaaccgg ggtagtaggc gggttattgc
     2641 cggcgaagcc gcggatatag gtgcgcgtct gatcgggcaa ggtcaagtag ccgtcggtgg
     2701 cgtagatata gaaatgccgg gtgcgcgccg ggatctcgcg atccggggcg ctcaggtcgg
     2761 cgtgcgccgg cccggtgacg gggatggcac gggcgctttg cggtagcaca acaccggccg
     2821 ccagcccacc ggccagcccg ccggccagtt tgagaaagct ccgccggtcc agggccggac
     2881 cgccgggcgt cgcgctttta ggggttctca tcgggtctct cctccgcata cgtgtaaaca
     2941 tctaccgaaa taataggccc ggaataccag tggggcaacc ggaaagagga tggtagtgac
     3001 ccatgtgcga agccgtgatc tcgccacctt tcacaataag ataggaactg gggcggcgcg
     3061 gatcacaggc ttctatatgc tttatcggca agttgcccgg ttattcttcc ccttgctggt
     3121 ggtaatgttt attggcaatc atagtgattg cgcgcaaacg atccttacct gctatataac
     3181 aggcgaaggt tacttcgcag gtaaatacag gtggacagat agtaaatgtg agttgaaggg
     3241 gatcgggtct atgaggtggt cagatgcgat ccccggcgcc ggcggtggta tgggacgccg
     3301 gaagggtcgc gaggataatc gggacgctat cagcagaccc ggcgacgagg atcggaccca
     3361 ccgccccggc ccccgacggc gccgtgcgcg tgagggggca tccttggggc aggcagggcc
     3421 gttacttcgc cagcagggcg cgtaactggt cctcgacctg gccgcgcagg gccaggtcat
     3481 tcgtgagcag caccactttc tgcagatcgg cgagcgcggc gtcgcggttg ccgctctggc
     3541 gatagctcag ggcgcggttg aaatagaacg tcgcgttatc gggctggagg cggatggcct
     3601 gggtgtagtc ggccagggcg tgcgggtagt cgccgcggcg ggcgtagatc aggccgcgcc
     3661 cgttaaaggc cccggcgttg ttcgcgtcga gggcgagggc ctgcgtgtaa tcggccaggg
     3721 cgcgggcatc gtcgccctgt gcgacgtagg cgtgcgctcg ccccaggtag cccgccgcgt
     3781 tggcaggcgc gatgcccagc gcgcgggaat agtcactcac cgcccggtcg taattgccct
     3841 gcgccaagta ggcgtcggcg cggcccaggt aggcatccgg caacaggggc gacacctgca
     3901 gcgcctggtt gtactcggca atagcgcggg tggcgtcgcc ctggctcgcg tagatgtcgc
     3961 cgcgccccag ataggcgccg gcggcacggg gattgtagtg taacgcctgc tcgaagtcgg
     4021 ccagggcgcg atccacgatc ccacgccgcc ggtagagatt gccgcgattg gtgtaggcca
     4081 cggccaggtc gggctggagc tggatggcct ggttgtaatc ggccagcgcc cgatccagcg
     4141 cgccctgcgc ggccagcgcc tcgccgcgcc cgttataggc gcccgcgtcg ttggggtgca
     4201 acttgatggc gctcgtatag tcggcgacgg cgtggtcgta ctggcccaac ccggcatagg
     4261 ccgccgcccg gtccacgtag agatcagcgt cctgtggttg gagcgcgagc gctttgttgt
     4321 actcggcaag cgcccggtca tacgcgccct gcgcgtaata ggtgcgtcca cgggtctgat
     4381 agccggcggc gagggtggcc ggggccggcc cggcgggggt gatagtgggg atcgcagtcg
     4441 gcgtagtgcg cggcggcggg gcctctttcg atgcctcttt aggtgcccct ttaggtgccc
     4501 ctttaggggc agtcgggggc ggggcggcga cgggggtggt ctcgaccggt aaggcgggcg
     4561 gagcggcggc ctcggaacag gccgcgccgc cgatcagcat gagacccagc agcagcaggc
     4621 ccggcaggcg ccccacccgc cccaggagcg atcgatgatc ggccatagta ggtagctccc
     4681 atcacgaacc gggatggaac tgttcggacg aactaccaca agagccaacc ttgtgtccgc
     4741 acgatacgac tattgtaccc gcgcggacta tatggcctat gaatagcggc gccggaaacg
     4801 ttacagcatt gttagcggtg cggcgccccc ctgccgcgga cgcggttcag cggccctcag
     4861 ccaccttacc ggtccgcaag aaatggggcg ccgcgcactc acgaggcgct ctcctcctgc
     4921 ggtgcgccgc ccacgatgag cgtgccgcag ttcatttcgg gcgccgggtg caggtgcatg
     4981 atgtccagga tagtcggcgt cacgtcgcag aggcgaccgg gccgcaggcg cacctggcgc
     5041 aggggcgagc cctccggcgc gaccaggatg aagggcaccg ggttgagcgt gtggtaggtg
     5101 tggggctcac cggtctcata gtcttccagt tgctcgcagt tgccgtggtc ggcggtgatc
     5161 agcgccacgc cgccctgcgc caggatggcc ggcaccaccc ggcccaggca ggtgtccacg
     5221 gtctcgacgg ctttgatggc cgcgctcagc ttgccgctat ggcccaccat atcgccattg
     5281 gcgtagttca cgacgatgaa gtcgta
//
LOCUS       DASUYC010001181         2326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1419565, whole genome shotgun sequence.
ACCESSION   DASUYC010001181 DASUYC010000000
VERSION     DASUYC010001181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1419565"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..775)
                     /locus_tag="VF276_19325"
     CDS             complement(53..775)
                     /locus_tag="VF276_19325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002628399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HEX6609061.1"
                     /translation="MAGNRSRNLSNAARRGRIVARPGLPEGAAQDTGAPPGIHRLDLD
                     SARDGLCYVPAGYRSDAPAPLVLLLHGAGGNARNGLAPLQPLADAAGLILLAPDSRRP
                     TWDVIQGGYGPDIAFIDAALAATFARYAVDPVHLGIGGFSDGASYALSVGLTNGDLFT
                     HILAFSPGFLAPASQNGAPRIFIAHGIHDSVLPIDQCSRRIVPRLRDAGYAVRYEEFD
                     GPHTVPPAIARAAVQWFTPVAA"
     gene            1162..>2326
                     /locus_tag="VF276_19330"
     CDS             1162..>2326
                     /locus_tag="VF276_19330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HEX6609062.1"
                     /translation="MQNPAWAGTDRRRREWVRYGSAVLAVALALAISLVLGELNVQSP
                     FTIFLTAVAVAAWFGGPGPGLLATILAGAILFVVFVPPVFTLSITSPIVLVRLAVFAV
                     GALLINLLGEARARSEAAARSAAARFAVTLGSIGDAVIATDVLGRVTFLNAAAETLTG
                     WRQAEALGHPAGEVFRLVDSQTREPLDTPVNLALGTGQTQRVVGMPLCLARDGTTHPV
                     DDSAAPIRDAAGQVRGVVLVFRDVTAQAAAEAERVAALEREQAARAEAEVAQARFALL
                     AEASGVLASSLDYQTTLAAVANLAVPRVADWCAVDLLDEQGIVRRLAVAHVDPAKVAW
                     AHELQERYPPDPNAAGGVYQVIRTGRPEILPDITPEMIRAATSDPDLLAIIDTI"
BASE COUNT          320 a          756 c          907 g          343 t
ORIGIN      
        1 gccgggccgg tttcaaaccg gcccctacaa tacggggttc agcgtccgcg ggctaggcgg
       61 cgaccggggt gaaccactgc accgcggcgc gggcgatggc gggtgggacg gtgtgcgggc
      121 cgtcgaactc ctcgtagcgc accgcgtagc cggcgtcgcg caggcggggc acgatgcgcc
      181 ggctgcactg gtcaatgggc aagacgctgt cgtggatgcc gtgcgcgatg aagattcgcg
      241 gggccccgtt ctggctcgcg ggggcgagga agcccggcga gaaggccagg atatgggtga
      301 agaggtcgcc gttggtcagg cccaccgaga gggcgtagga cgcgccgtcc gagaagccgc
      361 cgatgcccag gtggaccggg tccaccgcgt agcgggcgaa cgtcgcggcc agcgccgcgt
      421 cgatgaaggc gatatcgggg ccgtagccgc cctggatgac atcccaggtg gggcggcgcg
      481 agtcgggcgc gaggaggatc agcccggcgg cgtcggccag gggctggagg ggggccaggc
      541 cgttgcgggc attgccgccc gcgccgtgca gcagcagcac cagcggggcg ggcgcgtcgg
      601 accggtagcc ggcgggcaca tagcagaggc cgtcgcgcgc cgaatccagg tcgagccggt
      661 ggatgccggg cggggcgccg gtgtcctggg cggcgccttc gggtagccct gggcgggcca
      721 cgatgcgccc ccggcgcgcg gcgttactca aattacgtga tcgattgcct gccatgtccc
      781 ggtcctttcc cgtgttgcgc cgccgatccg gcacggatgg gtggagcaag atgcagaccg
      841 cctcggggcg gttgcccggc ccgtgaacgg gcgggcaacg gggacgaagg ccgccggcgc
      901 ggcctatcct ggggcgggtt cgcgcgattt attgtatggc ggcgcgccgc gggaagcgtg
      961 gggttggcgg cagactctgc cgagcgggtg gcgcccggcg gcagaatatg gtagagttgc
     1021 ctgccaattg tcgcaaatga gggcatggcc cttgctggct ttccatggcc ccggtatcac
     1081 ccggcccggc ggatagcgtg tggatcacgg tttggctgac gccgcctggg ccaatctact
     1141 ccctggagca cgaggcaatc tatgcaaaat ccggcatggg cgggcacgga ccgccggcga
     1201 cgcgagtggg tgcgttacgg cagcgcggtg ctggccgtgg ccttggccct cgccatatcg
     1261 ctcgtcctgg gcgagttgaa cgtccagagt ccgttcacga tcttcctgac ggcggtggcc
     1321 gtggccgcct ggttcggggg gccggggccg gggttgctgg cgaccatcct ggccggcgcg
     1381 atcctgtttg ttgtgtttgt gccgcccgtc ttcaccctaa gcattaccag tccaatcgtg
     1441 ctcgtgcgcc tggcggtgtt cgccgtgggg gcgctgctga ttaacctgct gggcgaggcg
     1501 cgggcgcgct cggaagcggc agcgcggagc gcggcggcgc gcttcgcggt gacactgggc
     1561 agcatcggcg acgccgtgat cgccaccgat gtgctggggc gagtcacctt tctgaatgcc
     1621 gccgccgaaa cgctgaccgg gtggcggcag gccgaggcgc tgggccaccc ggccggcgag
     1681 gtgttccggc tggtggatag ccagacgcgc gaaccgctgg ataccccggt gaacctggcc
     1741 ctgggcaccg gccagactca gcgggtcgtg gggatgccgc tctgcctggc gcgcgacggc
     1801 accacgcacc cggtcgacga cagtgccgca ccgatccgcg atgccgccgg gcaggtgcgg
     1861 ggcgtggtgc tggtgttccg cgatgtcacc gcacaggcgg cggcagaggc cgagcgcgtg
     1921 gcggcgctgg agcgcgagca ggcggcacgc gccgaggccg aggtcgccca ggcccgcttt
     1981 gccctcctgg ccgaagccag cggcgtgctg gcctcgtcgc tcgactatca gacgacgctg
     2041 gccgccgtgg cgaacctggc cgtgccacga gtggccgact ggtgcgccgt cgatctgctg
     2101 gatgagcagg gcatcgtgcg gcggctggcg gtagcccacg tcgacccggc caaggtggcc
     2161 tgggcgcacg agttacagga acgctacccg cccgatccca acgcagccgg cggcgtctac
     2221 caggtgatcc gcaccggccg gcccgagatc ctgcccgaca ttacccccga gatgatccgg
     2281 gcggccacgt cggaccccga cctgctggcg atcatcgaca ccatcg
//
LOCUS       DASUYC010001182         2417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_910597,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001182 DASUYC010000000
VERSION     DASUYC010001182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910597"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VF276_19335"
     CDS             complement(<1..373)
                     /locus_tag="VF276_19335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6609063.1"
                     /translation="MSENEQAGSSALPAGMLLGGMALAGAAAVGGTALLRRFDAWALR
                     MITHPERRDVGVTPADQGLAYEEVWLRSDDAVDLYGWLIPAPGGAQHARATLILGHGY
                     SDTIDSILGPVTYLQPAGYNVL"
     gene            complement(601..2247)
                     /locus_tag="VF276_19340"
     CDS             complement(601..2247)
                     /locus_tag="VF276_19340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6609064.1"
                     /translation="MSDRKTFAPMPLTRRRRPRALFVLLLLAVLTLVPPARPGAAAPA
                     SAALPFDLYSSFLWGVGTEADQVGDTPWLHDTPARILTCWFNRHEDLQVFKYWHDVGH
                     LARWQMDGYIIHVVTWEPLPITHGSYHTSAQWLDDISTLAGYLAGRPGLPQPPTVLWS
                     LATELQTYAQPENVWNDQTAPYYTKLQANLLAARDRIKAQAPGSLVSLSWGGWQTSFD
                     DPRIGAGLSMIPHFADTMRQMDFVSFQAMQSNAGMNAAEIARNAAYFHQYNPHLMISH
                     WKPDNNNIDVLKQDLKDFAQPGFIDTLRKNGVFAMSIMDQTYINRPDVLPAVQDLIRQ
                     TTDLRLVPNALPAGPQPGHPAWQPVGAFTDTADRRFFREVGHSLDHPFLDYWTSHGGL
                     PVLGYPLGEAHEEISPTDGKPYLVQYFERDRLEYHPANPPGARIVHGLLGRDVTSNRV
                     DGPFAGIGAPTDGSLYFAATGHTVAAAFRDYWQQHGGVSGFGYPISEPMDAVSPTDGK
                     TYRVQWFERARLEWHPDLPPPYRVSLGLLGREYLQARGIR"
BASE COUNT          367 a          774 c          877 g          399 t
ORIGIN      
        1 tcagcacgtt gtacccggcg ggctggagat acgtgacggg gccgaggatc gagtcgatgg
       61 tatcgctgta gccgtggccg aggatcagtg tggcccgcgc gtgctgcgcg ccgcccggcg
      121 cggggatcag ccagccgtag aggtccacgg cgtcgtcgct gcgcagccag acttcctcgt
      181 aggccagccc ctggtcggcg ggcgtgaccc cgacatcgcg gcgctcaggg tgggtgatca
      241 tgcgcagcgc ccaggcgtcg aagcggcgca gcagcgccgt gccgcccacc gcagcggccc
      301 cggccagggc catgccgccc agcagcatcc cggcgggtag cgcgctgcta cccgcctgct
      361 cgttctcgct caccctgctc tgcctcccct gctgtcgaat ctggccgcgt ggtcatccca
      421 ctgtacggat agcatacatc tgccctgcgc aaagatcaag ccgcccgacc aacctaaccc
      481 cctggccccc ttcccgccgc gggaaggggg aacggcaact cccctctccg cggcggagag
      541 gggccggggg agaggttgtc cacgcgccgg gatgcaacac ccccgcggtt attgccgccc
      601 tcagcggatg ccgcgcgcct gcaagtactc gcggcccagc agccccaggc tgacgcggta
      661 ggggggcggg aggtcggggt gccattcgag gcgcgcccgc tcgaaccact gcacgcgata
      721 ggtcttgccg tcggtggggc tgaccgcgtc catcggctcg ctgatcgggt agccgaaccc
      781 gctcacgccg ccgtgctgct gccagtagtc gcggaaggca gcggccaccg tgtgcccggt
      841 cgcggcgaag tagaggctcc cgtcggtggg ggcgccaatc ccggcgaagg ggccgtcgac
      901 ccggttgctg gtcacgtcgc ggcccaacaa gccatgcacg atgcgcgcgc cgggcgggtt
      961 cgcggggtgg tattccagcc ggtcgcgctc gaagtattgc accaggtagg gcttgccgtc
     1021 ggtcgggctg atctcctcgt gcgcctcgcc cagcgggtag ccgagcaccg gcaggccgcc
     1081 gtggctggtc cagtagtcga ggaaggggtg atcgagcgag tggcccacct cgcggaagaa
     1141 gcggcggtcg gcggtatcgg tgaaggcgcc caccggctgc caggccgggt ggcccggttg
     1201 cggcccggcg ggcagcgcgt tcggcaccag gcgcaggtcg gtcgtctgcc ggatcaggtc
     1261 ctgtacggcg ggtagcacat cgggccggtt gatatacgtc tggtccatga tcgacatggc
     1321 gaagacgccg ttcttgcgca gggtgtcgat aaagcccggc tgggcgaaat ccttgagatc
     1381 ctgcttgagc acatctatat tgttgttgtc gggcttccag tgcgagatca tcaggtgcgg
     1441 gttgtactgg tgaaaatagg cggcgttgcg cgcgatctcc gccgcgttca tgccggcgtt
     1501 gctctgcatg gcctggaagc tgacgaagtc catctggcgc atggtgtcgg cgaagtgcgg
     1561 gatcatgctc agcccggcgc cgatgcgcgg gtcgtcgaac gaggtttgcc agccgcccca
     1621 actgagcgag acgagcgagc cgggcgcctg ggccttgatg cggtcgcggg cggccagcag
     1681 gttggcctgc aacttcgtgt agtagggcgc ggtctggtcg ttccagacgt tctccggctg
     1741 ggcgtaggtc tgcaactcgg tcgccagcga ccagaggacg gtgggcggct gcggcaggcc
     1801 gggccgcccg gccaggtagc cggccagcgt gctgatgtcg tcgagccact gcgcggacgt
     1861 gtggtagctg ccgtgcgtga tcggcagcgg ttcccaggtg acgacgtgga tgatgtagcc
     1921 gtccatctgc cagcgggcca ggtgccccac gtcgtgccag tacttgaaca cttgcagatc
     1981 ttcgtggcgg ttgaaccagc aggtcaggat gcgggccggg gtgtcgtgca gccagggcgt
     2041 gtcgcccacc tggtcggcct ccgtgcccac gccccacagg aaggagctat agagatcgaa
     2101 gggcagggcg gcggatgccg gggccgccgc accggggcgg gcaggcggca ccagggtgag
     2161 cacggccagc agcaagagga cgaacagcgc gcgcggacgg cgccggcgag tcaagggcat
     2221 aggggcaaag gtctttctat cgctcattag tccgcgccac tcatttgtca ctctgagcgc
     2281 agcgaagagt cccgtctctt gagctataag acggagattc ttcgctgcgc tcagaatgac
     2341 agatccccgt tggccagcta cgcgcgctcc gtctgagttg taaccttccc ggtgtccggt
     2401 ccgttgatag agagaga
//
LOCUS       DASUYC010001183         4321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_216187,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001183 DASUYC010000000
VERSION     DASUYC010001183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4321
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216187"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VF276_19345"
     CDS             complement(<1..366)
                     /locus_tag="VF276_19345"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX6609065.1"
                     /translation="MPATDTITCYTNDGRAVVVPRDQVSMRPGVYGVAFTADRGQVLL
                     TRDRRSNVWELPGGGIDPGESAATAMVREFKEEVGLDITLGPPIYFAEAFYYHDKDTA
                     PGGWHSLKLFYLVHTSGTLD"
     gene            complement(457..1932)
                     /gene="cysS"
                     /locus_tag="VF276_19350"
     CDS             complement(457..1932)
                     /gene="cysS"
                     /locus_tag="VF276_19350"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX6609066.1"
                     /translation="MAVQLYNTMTRTKEPFAPLDPPHVGIYTCGPTVYNYQHIGNMRT
                     YIFEDLLVRVLAYNGYDVKRVLNITDVGHLTSDADEGEDKMEVGARRTGKTPAEIARF
                     YTDVFFEDFKALNCLQPTVVSPATEHIPEMLDLVGCLVDQGVAYEISDGVYFDVAKFP
                     AYGALSRLSLEGQEAGARVEVNPEKHGPFDFALWKKADPAHLQQWPSRWGQGYPGWHI
                     ECSAMSMKYLGKTFDIHCGGIDHIPVHHENEIAQSEACTHQTFARYWLHGEFLRMVQP
                     ITRERAGETVTVEEEVKMSKSLGGFITLSDVVKAGYPALAYRYFCLNAHYRSALTFRN
                     DLSSLDAPRTALNNLYDFVRRAASERGSAAEAEQAAVESPAWVQPYREQFLDAINDDL
                     NMPRALAAVWLLIGEANRRGEAPAALPVLFDWDNVLGLNLLQEAEREDVLEPDLQQMV
                     DERQAARKARDWARADALRAALLARGIEIEDTPQGPRWKRV"
     gene            2191..4272
                     /locus_tag="VF276_19355"
     CDS             2191..4272
                     /locus_tag="VF276_19355"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX6609067.1"
                     /translation="MDARREYGRANRAKQATDSVARQTAIERRRERAANEQFTIVAER
                     PDEVLGFYTVTGDSGIPYTVEIRDPDRFLNRCSCPDYEGNNLGTCKHIEAVLIRARRR
                     PAKLRAARTALVEQRRLHIRIGLAYVNGSWQLAPDYDESIDLGLLRLVNYYLAPHLDE
                     INRDPAAFAFRLKEFEDEVREYGGSASIDPDVWTHVAQVGHRLERAAAREALLAAVQA
                     GERRLDLLRVPLYPFQLLGVLFLAFTERALLADDMGLGKTAQAIGAAHLLMEQQGIGR
                     VLIITPASVKRQWAREIARFSGLPATVVGGSPARRARQYADPAVFTIINYELVLRDFE
                     RIVALAPDLIVLDEAQRIKNWQAKTTRRIKELPAAFAFVLTGTPLENRLEELYSVTEF
                     LDPRLLGPAWQFLAEHTEQDSFGAIIGYKNLEQVRHTLAPIFLRRRKADVLTELPPRV
                     DNFYTVDLTPEQTRLYRPIERELLDLLRLSRWDWEARGRVLECITHLRELADAAQLVA
                     PEVAASSKLVELGNIVPDLLATGHKILVFSEWERMTRLAQEALARQNIASVRLHGGMG
                     PAAREEAMRRFREEPDLGVFLSTEAGGLGLNLQVADYVINLDLPWNPARLEQRIARAH
                     RMGQTSTVNVINIVAAGTIEQRVLDVLYEKQALFTELFDTDIDTINLDAAAQLDRFRA
                     LVAQLLAHEGR"
BASE COUNT          699 a         1477 c         1449 g          696 t
ORIGIN      
        1 gtcgagcgtg ccgctggtgt ggaccaggta gaacagcttg agcgagtgcc acccgccggg
       61 ggccgtgtcc ttatcgtggt agtagaacgc ttccgcaaaa taaatcggcg ggccgagggt
      121 gatgtccagg cccacttcct ctttgaactc gcgcaccatg gccgtcgccg ccgactcgcc
      181 cggatcgata cccccgcccg gcaactccca gacgttggag cgccggtcgc gcgtcagcag
      241 cacctggccg cggtcggcgg taaaggcgac gccgtagacg ccggggcgca tgctcacctg
      301 gtcgcggggc acaaccaccg cgcgcccgtc gttggtgtaa caggtaatcg tatcggttgc
      361 aggcatgaaa atacatccct ttaatgggtc gcgcgttgcc gccggcgggc ggggatgtag
      421 ggatggcatc cgcccgcgac gcaacgcgcg atagggctag actcgtttcc agcgcgggcc
      481 ttgcggcgtg tcctcgattt cgataccgcg ggccagcagt gccgctcgca gcgcgtcggc
      541 gcgcgcccag tcgcgggcct tgcgggccgc ctggcgctcg tccaccatct gctgcaggtc
      601 gggttccagc acgtcctcac gctcggcctc ctgcagcaag ttcaggccca gcacattgtc
      661 ccagtcgaac agcaccggca gggcggcggg cgcctcgccc cggcggttgg cctcgccgat
      721 caagagccag acggccgcca gcgcgcgcgg catgttcagg tcgtcgttga tggcgtccag
      781 gaactgctcg cggtacggtt gcacccacgc ggggctttcc acggcggcct gttcggcctc
      841 ggcggccgag ccgcgctcgc tcgccgcgcg gcggacgaag tcgtacaggt tgttcagcgc
      901 cgtgcgcggg gcgtcgagcg agctcagatc gttgcggaag gtcagggcgc tgcgatagtg
      961 cgcgttcagg cagaagtagc gataggccag cgcgggatac ccggctttca ccacgtcgct
     1021 cagggtgatg aagccgccca ggctcttgct catcttcact tcttcctcga ccgtcaccgt
     1081 ctcgcctgcg cgctcgcggg tgatcggctg gaccatgcgc aggaactcgc cgtgcagcca
     1141 gtagcgcgcg aacgtctggt gtgtgcaggc ttcggactgg gcgatctcgt tctcgtggtg
     1201 gaccgggatg tggtcgatgc cgccgcaatg gatgtcgaac gtcttgccga gatacttcat
     1261 gctcatcgcc gagcattcga tgtgccagcc gggatagccc tggccccagc ggctgggcca
     1321 ctgttgcaga tgggccgggt cggccttctt ccagagcgcg aaatcgaacg ggccgtgctt
     1381 ctccgggttc acctcgacgc gggcgcccgc ctcctgccct tcgagcgaca ggcggctcag
     1441 cgcgccgtag gccggaaact tcgccacgtc gaaatagacg ccgtcgctga tctcgtaggc
     1501 cacgccctga tcgaccaggc agcccaccag gtccagcatt tccgggatat gctccgtggc
     1561 cggggaaacc accgtgggtt ggaggcagtt cagcgccttg aagtcctcga aaaacacgtc
     1621 ggtatagaag cgggcaattt cggcaggggt cttgccggtg cgccgggcgc cgacctccat
     1681 cttgtcctcg ccttcgtcgg cgtcggacgt gaggtggccc acgtcggtga tgttgagcac
     1741 gcgcttcaca tcgtagccgt tgtaggccag cacgcgcacc agcagatcct cgaaaatgta
     1801 ggtccgcata ttgccgatat gctggtagtt gtagaccgtc ggaccgcagg tgtagatgcc
     1861 gacatggggc gggtcaagcg gagcaaaggg ctccttggtc cgggtcatgg tgttgtaaag
     1921 ctgtacggcc atgtgtgtgc ctctcttccg caggtagtgt gcgctgcccg gcccgtaaac
     1981 gggcgggcta cggggacgaa gcccgcctac gcgggctgga tactccggcg gaggccgcgc
     2041 aggcggcctt ggttcccata gcccgcccct tgaggggcgg agcgcgcttg caccctggta
     2101 ttgccggaat tatactctat agagcgcgcc ttgagtagac ttctctagac cgatattcag
     2161 gggggtccaa ggccggggga tggaggaccg atggacgcca ggcgcgagta tggccgcgcg
     2221 aaccgggcga agcaggccac cgacagcgtg gcccgccaga cggcaatcga gcgccgccgc
     2281 gagcgcgcgg ccaacgagca gttcaccatc gtggccgagc ggcccgacga ggtgctgggc
     2341 ttctacaccg tcaccggcga cagcggcatc ccctacaccg tcgagatccg cgaccccgac
     2401 cgcttcctga accgctgttc ctgccctgac tacgagggca acaacctggg cacctgcaag
     2461 catatcgagg cggtgctgat ccgcgcccgc cgccgcccgg ccaagctgcg cgccgcgcgc
     2521 accgccctgg tcgagcagcg ccgcctgcac atccgcatcg gcctggccta cgtgaacgga
     2581 agctggcaac tggcccccga ctacgacgag tcgatcgacc tgggcctgtt gcgcctggtc
     2641 aactactacc tggctcccca cctggacgag atcaaccgcg accccgccgc cttcgccttc
     2701 cggctcaagg agttcgagga cgaggtgcgc gagtacggcg gcagcgcgag catcgacccc
     2761 gatgtctgga cgcacgtggc ccaggtgggc catcggctgg agcgggccgc ggcgcgcgag
     2821 gcgctgctgg cggcggtgca ggcaggcgag cggcggctgg acctgctgcg ggtgccgctc
     2881 taccccttcc agttgctggg cgtgctcttc ctggccttca ccgagcgggc gctgctggcc
     2941 gacgacatgg gcctgggcaa gacggcgcag gccatcggcg cggcgcacct gctgatggag
     3001 cagcagggca tcgggcgcgt gctgatcatc accccggcgt cggtcaagcg gcagtgggcg
     3061 cgcgagatcg cccgcttctc gggcctgccc gccacggtgg tcggcggcag cccggcccgc
     3121 cgcgcccgcc agtacgccga tcccgccgtg ttcaccatca tcaactacga actggtgctg
     3181 cgcgacttcg agcgcatcgt ggccctggcg cccgacctga ttgtgctaga cgaggcccag
     3241 cgcatcaaga actggcaggc caagaccacc cgccgcatca aggaattgcc cgcggcgttc
     3301 gccttcgtgc tcaccggcac gccgctggag aaccggctgg aggagctata cagcgtgacc
     3361 gagtttctcg acccgcgcct gctcggcccg gcctggcagt tcctggccga gcacaccgag
     3421 caggactcgt tcggggcgat catcggctac aagaacctgg agcaggtgcg ccacaccctg
     3481 gccccgatct tcctgcgccg ccgcaaggcc gacgtgctga ccgaactccc gccgcgcgtg
     3541 gacaacttct acaccgtgga cctgacgccg gagcagaccc ggctctaccg cccgatcgag
     3601 cgcgaactgc tcgacctgct gcgcctgagc cgctgggact gggaggcgcg aggccgcgtg
     3661 ctggagtgca tcacccacct gcgcgaactg gccgacgccg cgcaactggt cgcgccggag
     3721 gtggccgcgt cgagcaagct ggtggaactg ggcaacatcg tccccgacct gctggcgacg
     3781 gggcacaaga tcctggtctt cagcgagtgg gagcgcatga cccgcctggc gcaagaggcg
     3841 ctggcgcggc agaacatcgc cagcgtgcgc ctgcacggcg gcatggggcc ggcggcccgc
     3901 gaggaggcga tgcgccgctt ccgcgaggag ccggacctgg gcgtcttcct ctccaccgaa
     3961 gcgggcgggc tgggcctcaa cctgcaagtg gccgactacg tgatcaacct cgacctgccc
     4021 tggaacccgg cccggctgga gcagcgcatc gcccgcgccc accgcatggg ccagaccagc
     4081 acggtcaacg tgatcaacat cgttgccgcc ggcaccatcg agcagcgcgt gctcgacgtg
     4141 ctctacgaga agcaggccct cttcaccgaa ctcttcgaca ccgacatcga cacgatcaac
     4201 ctcgacgccg ccgcccaact cgaccgcttc cgcgccctgg tcgcgcaact gctggcgcac
     4261 gaggggcggt aagaatctgg aacacttgtc attactcagg atggatcagc tatttaaagc
     4321 g
//
LOCUS       DASUYC010001184         2026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_448281,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001184 DASUYC010000000
VERSION     DASUYC010001184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448281"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..1518
                     /locus_tag="VF276_19360"
     CDS             154..1518
                     /locus_tag="VF276_19360"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012329916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HEX6609068.1"
                     /translation="MATLLVRNATLLVTMDDEDHRWPDGAIYVVDNRIEAVGPAADLP
                     ATADRVIDATDMVILPGLVNTHHHFYQTLTRNVPGTQDAVLFQWLVTLYPLWARLTPE
                     AVYVSSKIAIAELMLSGCTTSSDHTYIWPNGARLDDQIQAAQEMGFRFHAARGSMSVG
                     ESQGGLPPDSVVEDEAAILADSRRVIEQYHDAGRYAMLRIVLAPCSPFSVSQDLMRES
                     LALARSYGVHSHTHLAETRDEEVYCLANFGRRPVELAEDLGWMGTDVWHAHMVHPSAE
                     EVGRLGASRTGVAHCPSSNMRLASGIAPVPGLLGAGARVGLGVDGSASNDSSHMLAEA
                     RQALLLHRVMGDPAGMTARAALRLATRGGAAVLGRDDIGYLAPGMAADFIGYRLDTLG
                     LAGGAVHDPLAALVFCHPPQVDLSVINGRVRVADGVLLDVDLPPLIARHNALARALVR
                     GEPA"
     gene            complement(1515..>2026)
                     /locus_tag="VF276_19365"
     CDS             complement(1515..>2026)
                     /locus_tag="VF276_19365"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6609069.1"
                     /translation="ILDVFTAAFAGPPYHQGLPDTARFNATLTRHARYTGFRCYVAEE
                     GAGGRIVGFTYGYTALPGGWWYDLVAAALDRKTVDRWMGDAFEFVELAVHPDAQGHGL
                     GARLHDTLLADLPYRTALLSTYQGETAARQLYRRRGWVPLLEHFYYPDGSVPMAILGL
                     KLPLRPARG"
BASE COUNT          296 a          705 c          739 g          286 t
ORIGIN      
        1 aggttggccg gcgatcggga tgccgccctt cgcgcgcata aacgtgacgc gcccccgctg
       61 ccggggtggt ttgacgaggc tgctgggcgc tattatacta taccttccgc gagaatgcgc
      121 aacccctacc ccgccgggag atcggaggac acgatggcga cattgctggt caggaacgcg
      181 acgctgctcg tgaccatgga cgacgaggac caccgctggc ccgacggcgc catctacgtg
      241 gtcgataacc gcatcgaggc ggtgggaccg gccgccgatc taccggccac cgccgaccgg
      301 gtgatcgacg ccacggacat ggtcatcctg ccggggctgg tcaacacgca ccaccacttc
      361 taccagaccc tgacccgcaa cgtgccgggc acgcaggacg ccgtgctgtt ccagtggctg
      421 gtcacgctct acccgctgtg ggcgcggctg acgccggagg ccgtttacgt cagcagcaag
      481 atcgccatcg ccgagttgat gctgtcgggc tgcaccacgt ccagcgacca cacctatatc
      541 tggcccaacg gcgcgcggct ggacgaccag atccaggcgg cgcaggagat gggcttccgc
      601 ttccacgcgg cgcgcggctc catgtcggtg ggcgagtcgc agggcgggct gccgccggac
      661 agcgtggtgg aggacgaggc ggcgatcctg gccgactcgc ggcgggtgat cgagcaatac
      721 cacgacgcgg ggcgctacgc catgctgcgt atcgtgctgg cgccctgttc gcccttctcg
      781 gtgtcgcaag acctgatgcg cgagagcctg gccctggccc ggtcctacgg cgtgcactcg
      841 cacacccacc tggccgagac gcgcgacgag gaggtctact gcctggcgaa cttcgggcgg
      901 cgcccggtgg aactggccga agacctgggc tggatgggca ccgatgtctg gcacgcgcac
      961 atggtccacc cgtcggcgga ggaggtgggg cggctgggcg cgagccggac cggggtggcc
     1021 cactgcccgt cgtccaacat gcggctggcc tcggggatcg cgccggtgcc ggggctgctg
     1081 ggggccgggg cgcgggtggg tctgggcgtg gacggctcgg cgtcgaacga cagctcgcac
     1141 atgctggccg aggcccgcca ggcgctgctg ctgcaccgcg tcatgggcga tcccgccggg
     1201 atgacggcgc gcgcggcgtt gcggctggcg acgcgcggcg gggccgcggt gctgggccgc
     1261 gacgacatcg gctatctcgc ccccggcatg gccgccgact tcatcggcta ccgcctcgac
     1321 acgctgggcc tggccggcgg cgccgtccac gacccgctgg cggcgctggt cttctgccac
     1381 ccgccgcagg tggacctcag cgtgatcaac gggcgggtgc gggtggcgga cggcgtgttg
     1441 ctcgacgtgg acctgccgcc gctgatcgcc cgccacaacg ccctggcccg cgccctcgtg
     1501 cgcggcgagc cggcctagcc acgggcgggg cgaaggggca acttcagccc caggattgcc
     1561 atggggacgc tgccgtcggg gtaatagaaa tgctccagca gcggcaccca gccccggcgc
     1621 cggtaaagct gccgcgccgc cgtctcgccc tggtaggtgg acagcagcgc cgtccggtac
     1681 ggcaggtccg ccagcagcgt gtcgtgcagc cgggcgccga gaccgtgccc ctgggcgtcg
     1741 ggatgcacgg ccagttccac aaactcgaag gcgtcgccca tccacctgtc cacggtcttc
     1801 cgatcgagcg cggcggcgac gagatcgtac caccagccac cgggcagcgc ggtatagccg
     1861 taggtgaagc ccacgatgcg gcccccggcg ccctcttccg cgacgtaaca gcggaatccc
     1921 gtgtaccgcg cgtgccgggt cagcgtggca ttgaaccggg ccgtgtcggg cagcccctgg
     1981 tggtaaggcg gcccggcaaa ggcggcggta aacacgtcga ggatgc
//
LOCUS       DASUYC010001185         2685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_2066322, whole genome shotgun sequence.
ACCESSION   DASUYC010001185 DASUYC010000000
VERSION     DASUYC010001185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2066322"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="VF276_19370"
     CDS             complement(<1..718)
                     /locus_tag="VF276_19370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HEX6609070.1"
                     /translation="MRTHLLSPARRMAAGLLILLLPALTACGAEAPTTPAAAGQARSI
                     GAGAPTGITAPAAAATDTAAPAIADTAQLRAVQPLAASTVAAAEAPPPTPLPAPPAAN
                     AFIATAADPLSTFGMDVDTASYTNVRNYLNAGQLPPPALVRAEEFINYFHYPYPAPTD
                     GPFGITVDGAPSPFTAGETQIVRVGIQGRRIDPAQRQDAVLTFVIDVSGSMDQPNRLP
                     LVKDSLRALVQQLRPTDQVAI"
     gene            1092..>2685
                     /locus_tag="VF276_19375"
     CDS             1092..>2685
                     /locus_tag="VF276_19375"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEX6609071.1"
                     /translation="MTRLIVLIALLVTLPGVSVGAAPQPAAAAGAAATPTACAAGQFS
                     DVPPESTFYPWITHLAGVGAISGYSDCTFRPGNTITRGQVAKVIMLAFAIPLVNPPQA
                     TFSDVPIGSPFFTYIETAYAHGIISGYADGTFHPADNVTRGQLTKMVARGRNWPLLNP
                     AQPTFTDVPPAHPFFTYIETSYAHGVISGYTCGAACLEFRPGNTSTRGQGAKIIDIAA
                     TADATATPAATGTAVPATGTATATATPDAGPKGTATATAGSVPSATGTATATSTAPPV
                     TGTATPKVSATRTPTYTATATTTSTDTPTNTPTDTATATNTPTSTPTDTPTSTPTDTP
                     TNTPTDTPTNTPTNTPTATPTHTATPTNTPTNTSTPTNTPTDTPTNTPTNTPTNTATA
                     TNTPTDTPTLTPTNVATSTPTATPTRTSMPTRTPTSTRTPTRTPTNTPTSSVTPDVPV
                     LLNVGYSSGTVYQGGAITLSYTVYTPYSGVFALGANVRNNNHGYSDVGNDKQVSLPAG
                     TSVVTRQFYIAPNYAAGTYDILW"
BASE COUNT          473 a          988 c          856 g          368 t
ORIGIN      
        1 cgatggcgac ctggtcggtc gggcgcagtt gttgcaccag ggcgcgcagc gaatctttca
       61 ccaggggcag ccggttcggc tggtccatcg agccgctgac atcgatcacg aaggtgagca
      121 cggcgtcctg gcgctgggcc gggtcgatgc gccggccctg gatgcccacg cgcacgatct
      181 gcgtctcgcc cgcggtgaag ggcgacggcg cgccgtccac ggtgatgccg aagggcccgt
      241 cggtgggcgc gggataggga tagtggaagt agttgatgaa ctcttcggcg cgcaccaggg
      301 cgggcggggg gagttggccc gcgttgaggt agttacgcac gttggtatag gacgccgtat
      361 cgacatccat gccgaaggtc gagagcggat cggcggcggt ggcgatgaag gcgttcgcgg
      421 cgggcggtgc gggcaggggc gtgggcggcg gcgcctcggc ggcggcgacg gtactcgcgg
      481 cgagaggctg gaccgcgcga agttgggccg tatccgcgat agcgggcgcg gcggtgtcgg
      541 tggccgcggc ggccggggca gtgatgccgg tgggagcgcc ggccccgatg ctgcgcgctt
      601 gcccggcggc agcaggggtg gtgggggctt ccgcgccgca cgcagtcagg gccggcagga
      661 gcaggatcag cagacccgcg gccatacggc gggcaggact cagtagatgg gtgcgcatac
      721 gatcctcctt tggacgagct tgcaccatca gtcaccttgt cgtcttcacc tgcctgccca
      781 tggtacaggc gagttagtgg gatgacgcga ggtcgccgcc gattgttccc cgccgccacc
      841 gctgatttac cggcggttgc acagcgcagc gagggatgct tgcataatct tcaggattgg
      901 cgcgcgccgc agggggaatc tggacagatt gtaagcgctt cggcgcccgg ttgtaagcgc
      961 ctgtttcttg aggtcgcccc ggcggggtgc gtataatcag gcataccatc ccgaattacg
     1021 tcccgcctgg ccgggactcc gaaggagcag gcatgtccgg cgccccgcac tggtcttcac
     1081 ggtccactcg cttgacacga ctaatcgtac tgatcgcact actggtcaca ctgcccggcg
     1141 tgtccgtcgg cgccgcaccc cagcccgccg ccgccgcggg cgccgcggcc actcccacag
     1201 cctgcgccgc cgggcagttc agcgatgtgc cgcccgaatc gaccttctac ccctggatca
     1261 cgcacctggc cggggtgggc gccatttccg gctacagcga ttgcacgttc cggcccggca
     1321 acacgatcac gcgcgggcag gtcgccaagg tcatcatgct cgccttcgcc atcccgctgg
     1381 tcaacccgcc gcaggccacg ttcagcgatg tacccatagg gtcgccgttc ttcacctaca
     1441 tcgagacggc ctatgcccac ggcatcatct ccggctacgc cgacggcacc ttccacccgg
     1501 cggataacgt gacgcgcggg cagttgacga agatggtggc ccgcgggcgc aactggccgc
     1561 tgctcaaccc agcgcagccc acctttaccg acgtgccgcc cgcgcacccg ttcttcacct
     1621 acatcgagac cagctatgcc cacggcgtga tctcgggtta cacctgcggg gccgcgtgcc
     1681 tggagttccg gcccggcaat acctcgacgc gcggccaggg cgccaagatc atcgacatcg
     1741 cggccacggc ggacgccacc gcgacacccg ccgccaccgg caccgccgtc ccggccaccg
     1801 gcaccgccac ggcgaccgcc acgccggacg ctgggccgaa gggcaccgcc acggcgaccg
     1861 cgggcagcgt cccgtctgcc accggcaccg ccacggccac cagcacggca ccgcctgtca
     1921 ccggcacggc cacgcccaaa gtgtcggcca cccgcacgcc gacctatacc gccaccgcga
     1981 cgaccacgtc gacggatacg ccgaccaaca cgccgaccga taccgccacg gcgaccaata
     2041 caccgacgag caccccgaca gatacgccga cgagcacccc cacggacacg ccaaccaaca
     2101 ccccgaccga tacgccaacc aatacgccga ccaacacgcc gacggccacg ccgacgcata
     2161 ccgctacgcc gacgaatacg ccgaccaaca cctccacgcc cacgaacacg ccgacggata
     2221 cgccgaccaa cacgcccacc aacacgccga caaacacggc tacggccacc aatacgccga
     2281 cggatacgcc tacgctcacg ccgaccaatg tggcgacgag cacgccgacc gcgacgccga
     2341 cgcgcaccag catgccgacg cgcacgccca cctcaacgcg cacgccgacg cgcacgccca
     2401 ccaacacgcc gaccagttcg gtcacgcccg atgtgcccgt gctgctcaac gtgggctact
     2461 cgtcgggcac cgtgtatcag ggcggggcca tcactctgtc ctacacggta tacacgccct
     2521 acagcggcgt cttcgcgctg ggcgccaacg tccgcaacaa caaccacggc tatagcgatg
     2581 tcggcaacga taagcaggtc agcctgccgg cgggcacctc ggtcgtgacc cgccagttct
     2641 atatcgcgcc gaattatgcc gccggcacct acgatattct ctggg
//
LOCUS       DASUYC010001186         3328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1144242, whole genome shotgun sequence.
ACCESSION   DASUYC010001186 DASUYC010000000
VERSION     DASUYC010001186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1144242"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1170
                     /locus_tag="VF276_19380"
     CDS             <1..1170
                     /locus_tag="VF276_19380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6609072.1"
                     /translation="GEIFDGQGYGGQFYPANEPTIYVLADVQNVLIPKITQVYWWPIT
                     QEYKADWESLNQPLTGTLEIGTRSFPPTTYSLRHDNSYSNAQTELILGDEAAPAYAAY
                     QKALSDYQAAAGAYNEQWQVFQQQLAEWGQKADELRAAGKSTADLPVPKQPVQPTQPT
                     VSVTAPAPGIAFTLPAGEYQMALRGTDGTIVPGSERRIVAFGPRRSGVAYKVIAESKY
                     TLPDASTNPQDTIFVSGEKALYVQPAAQREYDNFYAAKLLNPQQRAAQDRRGVWTWLP
                     AGEFPATTLALSVGGATETITKVKYYAKQRPGAALGYDILPYDASAGEGVATFDAFKI
                     VPQRGTMTIAAPGTPDSSREIRVVQTGAGNVLIGLAFLPLAGGLAGLAYRRWRTR"
     gene            1167..3260
                     /locus_tag="VF276_19385"
     CDS             1167..3260
                     /locus_tag="VF276_19385"
                     /inference="COORDINATES: protein motif:HMM:NF039707.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HEX6609073.1"
                     /translation="MTYTVSSRALSVKIDPESGRLLRVRNLARDLDLIAGTPANPPFR
                     LELAKVGVVETFEDFHCEPLANGLRLIWRAPHSITVTSDILVRGDDILFTVAAANAGS
                     STIDRIEYPILGGIGRLGGAGQDDLAHSHATGMLFHDPLDLFEPDPENRRRLRFSPYP
                     EGFAGSTMQFMAYYARNRGGFFLGTEDGEKGLKWYNFYKENDFLGASILHKASVLAPG
                     RSVAPPYAVVFAPLLEGTWHEAGDRYRRWACRQAWAQPGPRSRWLREAVGVCTFGINA
                     RYDRAAWLDEIHRMAGTPVFHILGPNWAKWGHDYHNNLPRGRADWFPASFSAANMATI
                     ARNGDYWAPFEFDLLCSHSAEFADPVLENRMQHNDAELGLSDPGLVGFPFMCAGTEYW
                     HQFHVERDERLIDEYGADALYYDISVSNLLLQCLAPGHQHLPGAGTAIADAFTTMYRD
                     TSAAMARAKGAYVPAGTEVISEIFLNVFDYYQARAEAGPYAPFEHNPYREWLIAGQAE
                     KIPLFTYVFQERAPLRMDGWAKLAPEAGDLFYWTAAQVLLNGGLFELNYEFSALEDLN
                     GQSDDPAEHYYRFDARHHPIDPQKAAFVGEVARARVGPANRFLAYGHMLPAPAVDAPP
                     VDLSYYTYNVGKGDAAIYEDRGTMTVPGALASAWQHEGKTIWLVANLLPQAQEIRVDG
                     QTLTLPPRRIRVVEP"
BASE COUNT          577 a         1161 c         1093 g          497 t
ORIGIN      
        1 ggcgagatct tcgacggtca gggctacggg ggccagttct atcccgcgaa cgagccgacg
       61 atctatgtac tggccgacgt gcagaacgtg ctcatcccca agatcaccca ggtctactgg
      121 tggccgatca cccaggagta caaggccgat tgggagagcc tgaaccagcc gctgaccggc
      181 acgctggaga tcggcacgcg cagcttcccg ccgaccacgt acagcctgcg ccacgataac
      241 agctacagca acgcgcagac cgagttgatc ctcggcgacg aggcggcgcc cgcatatgcc
      301 gcctatcaga aagcgctgag cgactatcag gcggcggcgg gcgcgtacaa cgagcagtgg
      361 caggtattcc agcagcaact ggccgagtgg ggccagaagg ccgacgagct acgcgccgcg
      421 ggcaaatcca ccgccgacct gccggtgccc aagcagccgg tacagcccac ccagcccacc
      481 gtgagcgtca cggcgcctgc gccagggatc gccttcaccc tgccggcggg cgagtaccag
      541 atggcgctgc gcggcaccga cggcaccatc gtgccgggca gtgagcggcg catcgtggcc
      601 ttcggcccgc gccgcagcgg ggtcgcctac aaggtgatcg ccgagagcaa atacacctta
      661 cccgacgcca gcaccaaccc gcaggacacg atcttcgtca gcggcgagaa ggcgctctat
      721 gtgcagcccg ccgcccagcg tgagtacgac aacttctacg ccgccaagct gctcaacccg
      781 cagcagcgcg cggcgcaaga ccggcgcggc gtctggacct ggctgcccgc cggggagttc
      841 ccggccacca cgctcgcact cagcgtgggc ggcgcgacgg agaccatcac caaggtgaag
      901 tattacgcca agcagcggcc cggcgccgcg ctgggctacg atattctgcc ctacgacgcg
      961 agcgcgggcg agggcgtcgc gaccttcgac gccttcaaga tcgtgccgca gcgcgggacg
     1021 atgaccatcg cggcgcccgg cacccccgac agcagccggg agatccgcgt ggtgcagacc
     1081 ggcgccggga acgtgctgat cgggctggcc ttcctgcccc tcgccggggg gctcgccggg
     1141 ctggcctatc gccgctggag aacgcgatga cgtacactgt ttcgagccgc gcgctcagtg
     1201 tgaagatcga cccggaaagc gggcgcctgc tgcgtgtgcg caacctggcc cgcgacctgg
     1261 acctgatcgc cggcacgccc gcgaacccgc ccttccgcct ggaactggcg aaggtcggcg
     1321 tggtcgagac cttcgaggac ttccactgcg aaccgctggc gaacggcctg cgcctgatct
     1381 ggcgcgcccc gcacagcatc accgtgacca gcgacatcct ggtgcgcggc gacgacatcc
     1441 tgttcaccgt cgcggcggcc aacgcgggca gcagcaccat cgaccgcatc gagtatccga
     1501 tcctgggcgg catcgggcgg ctgggcgggg cggggcagga cgacctggcg cacagccacg
     1561 ccaccgggat gctgttccat gacccgctcg acctgttcga gccggacccg gagaaccgcc
     1621 gccgcctgcg cttctcgccc tatcccgaag gcttcgccgg gtcgaccatg cagttcatgg
     1681 cctactacgc ccgcaaccgg ggcggcttct tcctcggcac cgaggacggc gaaaagggct
     1741 tgaagtggta caacttctat aaagaaaacg attttctggg cgcgagcatc ctgcacaagg
     1801 cgtcggtatt ggcgccgggc cgctcggtgg cgccgcccta cgccgtggtc ttcgcgccgc
     1861 tgctggaggg gacatggcac gaggcgggcg accgctaccg ccggtgggcg tgccggcagg
     1921 cctgggcgca gccggggccg cgcagccgct ggttgcgcga agcggtcggc gtctgcacct
     1981 tcggtatcaa cgcccgctat gaccgggcgg cctggctgga cgagatccac cgcatggccg
     2041 gcacgccggt gttccatatc ctgggcccca actgggccaa atggggccac gactaccaca
     2101 acaacctgcc gcgtggccgc gccgattggt tcccggcctc gttcagcgcg gccaacatgg
     2161 cgaccatcgc ccgcaacggc gactactggg cgcccttcga gttcgacctg ctgtgcagcc
     2221 actccgccga gttcgccgac ccggtgctgg agaaccgcat gcagcacaac gacgccgagc
     2281 tgggcctgag cgatccgggc ctggtcggct tcccctttat gtgcgcgggc accgaatact
     2341 ggcaccagtt ccatgtcgag cgcgacgagc ggctgatcga cgagtacggc gccgacgcgc
     2401 tctactacga catctcggtg agcaacctgc tgctgcaatg cctggcgccc ggccatcagc
     2461 acctgccggg cgcgggcacg gccatcgccg atgccttcac cacgatgtat cgcgatacca
     2521 gcgcggccat ggcgcgggcc aaaggcgcct atgtgcccgc gggcaccgag gtcatcagcg
     2581 agatcttcct gaatgtattc gattactacc aggcgcgcgc cgaggccggt ccctatgccc
     2641 cgttcgagca caacccgtat cgcgagtggc tgatcgcggg ccaggctgag aagatcccgc
     2701 tgttcaccta tgtgttccag gagcgcgcgc cgctgcgcat ggacggctgg gccaagctgg
     2761 cgcccgaggc gggcgatctc ttctactgga ccgccgctca ggtgctgctc aacggcgggc
     2821 tgttcgagtt gaattacgag ttcagcgctt tggaagacct gaacggccaa tccgacgacc
     2881 ccgccgagca ttactaccgc ttcgacgcgc gccaccatcc tatcgacccg cagaaagcgg
     2941 ctttcgtcgg cgaggtggcc cgcgcccgcg tcggcccggc taatcgcttt ctggcctacg
     3001 gccatatgct gcccgcgccc gccgtggatg cgccgccggt ggatctgagc tattacacct
     3061 acaacgtcgg caaaggcgat gccgccatct acgaggaccg gggcacgatg acggtaccgg
     3121 gcgccctggc gagtgcctgg cagcacgagg gcaagacgat ctggctggtc gccaacctgc
     3181 tgccgcaggc gcaggagatc cgcgttgacg gccagacgct gacgctgccg ccgcgccgga
     3241 tccgcgtggt cgagccttaa gccgtagcga ggtcgcgtaa ttatccggta gttttacttc
     3301 agacgaagga gtgttatcgt agtggata
//
LOCUS       DASUYC010001187         4208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1606467, whole genome shotgun sequence.
ACCESSION   DASUYC010001187 DASUYC010000000
VERSION     DASUYC010001187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1606467"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VF276_19390"
     CDS             <1..627
                     /locus_tag="VF276_19390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020093975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEX6609074.1"
                     /translation="VACGNSICGNSAIAAVAPVIGATAADVASSIALTAVVGVVLVLA
                     LPLLIPVLQFSFYQYGVLAGMTVYAVPQVLAATFPVSALSGQIGTLVKLVRVLMLGPV
                     VFFFALRYPRPATAGGFALSRFVPWFIIGFIALAVARSIGLLPEALAAPAADLSRWLT
                     VAAMAALGLGVDIRVVSKVGRPIALAVIGSLAVLLVLSISLIIGLGIR"
     gene            705..1682
                     /locus_tag="VF276_19395"
     CDS             705..1682
                     /locus_tag="VF276_19395"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX6609075.1"
                     /translation="MESTTKNRKTREQVARMVARAFGGAALDAGDDAVSELKEGWFNA
                     AYNVRLAGGREVILKIAPPPDAEVLVYERNIMATEVATMRLVRANPAIPVPEIYYFDQ
                     ARDLCDSDYFFMEKLTGDNYEHVKESLPPAMQEQIDRQIGAIVREINGFTGTYFGYDG
                     NPALRGATWKEAFVKIVHSVLEDGRRKDADFGYPIDEIRAAVLQHAPALEAVTTPQLV
                     HWDAWNLNFFVKDGQVTGILDFERALWADPLLEAQFRALAFGGVSDSMRGYGKTTFTH
                     AEDERCHLYTLHLALVMKAECYYRNYETDEVSNIATQLIGPALTWLREN"
     gene            1707..3050
                     /locus_tag="VF276_19400"
     CDS             1707..3050
                     /locus_tag="VF276_19400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX6609076.1"
                     /translation="MERLGTAPVGKLLLEMSSQTTFSLLVYAVYSVTDTYFLSVGINS
                     LAAAGASIIAPVLTALGGVATTVGAGGASVVSRALGEANPQKASRTVANTFLIFWVVA
                     LAITVGGALFIQPIVYLLGATDSVAPYAVEYGRIIFLGALTSTGYSAIVRADGNIGYS
                     TAMWIVPVTANTVLCWLFVIVLHMGVAGAALATVLGQAISAGMSIYFFFLRKNRSYQI
                     KAAYFQPDWAIMGEVLIIGFPSFVKGLSTGLVGIVTNNLLRVIGGDSALGVFAIVNRL
                     YFALSTPQFGIMQGMQPILGYNFGRKQFGRVRQTITYSLGTAVVYGLIVCSLCLLIPT
                     TLVALLSKEPRIVAEGQDALRLMALAYPFAGISLMVAAYFQAVGRAKEALLITLGGIV
                     LVKLPVLLLASGLFSLTGIWAAEAASEFILCVGSLLMLRSYQEKMVTMERLAYSP"
     gene            3467..>4208
                     /locus_tag="VF276_19405"
     CDS             3467..>4208
                     /locus_tag="VF276_19405"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6609077.1"
                     /translation="MPDEEQISRLAQLGLNAYEARAYLALLGKESFSASQVADLSGVP
                     RQRIYDILASLVEQGLAVSRPGRRGTKYAAVAPGVALQALLGRAKQQVTALEVATNSL
                     ITTLASQYAAGKGETSPLEYIEVLRGTAAINQRFAEIQANCRREILVFTKPPYATPPQ
                     ENVEGLETLHRHVRACSIYEYDVLRDPATRDGVAYFLQQGEQARFVAHLPMKLVVVDE
                     AIVMFAMEDPIAGRTDLTIMVIEHPQLAA"
BASE COUNT          703 a         1292 c         1372 g          841 t
ORIGIN      
        1 gtcgcctgcg gcaactccat ctgcggcaac tcggccatcg ctgccgtcgc cccggtcatc
       61 ggcgccaccg ccgccgacgt ggcctcgtcc atcgcgctga ccgccgtggt cggcgtggtg
      121 ctggtgctgg ccctgccgtt gctgatcccc gtgctgcaat tctcgttcta ccagtacggc
      181 gtgctggccg ggatgacggt ttacgccgtg ccccaggtgc tggccgctac cttcccggtg
      241 agcgcgctga gcggccagat tggcacgctg gtcaagctgg tgcgcgtgct catgctgggg
      301 ccggtcgtgt tcttcttcgc gctgcgctac ccgcgccccg ccacggcggg cggcttcgcg
      361 ctgagccggt tcgtgccctg gttcatcatc ggcttcatcg ccctggccgt cgcgcgctcg
      421 atcgggctgt tgcccgaagc cctggccgcg cccgccgccg atctctcgcg ctggctgacg
      481 gtggccgcca tggccgcgct gggcctcggc gtcgatattc gtgtcgtcag caaagtaggc
      541 cggcccatcg ccctcgccgt gatcggctcg ctggccgtgc tgctggtgct gagcatcagc
      601 ctgatcatag ggttggggat tcgttagagg cctttgttgc cacggcgcga cgcagataca
      661 tgccggctca acctgggcct gctgttgacc aggagaggga ttccatggaa agcacaacca
      721 aaaaccgcaa gacgcgagag caggtggccc ggatggtcgc gcgggccttt ggcggggccg
      781 ccctggacgc cggggacgac gcggtgagtg agttaaagga aggctggttt aacgcggcct
      841 ataacgtccg gctggccggc gggcgggagg tgatcttaaa gatcgcgccg ccccccgatg
      901 ctgaagtgct ggtgtatgag cggaatatca tggcgaccga ggtggcgacg atgcgcctgg
      961 tgcgggcgaa cccggccatc cccgtgcccg agatttacta ttttgaccag gcccgcgatt
     1021 tatgcgactc cgattatttc ttcatggaga agctgaccgg cgataattac gagcacgtga
     1081 aagaatcgct gcccccggca atgcaggagc aaatcgaccg gcagatcggc gcgatcgtcc
     1141 gcgagatcaa cgggtttacg ggcacctatt tcggctatga cggcaacccc gccctgcgcg
     1201 gggctacctg gaaggaggcc ttcgtcaaga tcgtgcattc ggtgctggaa gacggtcgcc
     1261 ggaaagatgc ggatttcggc tatcccatcg acgagatccg tgccgccgtc ctgcaacacg
     1321 cgcccgccct ggaggcggtc actacgccac agttggtcca ctgggatgcc tggaacctga
     1381 atttcttcgt caaggacggc caggtcacag gcatcctcga ttttgaacgg gcgctctggg
     1441 ccgacccctt gctggaggcc caattccggg cgttagcctt tggcggcgtc tccgacagca
     1501 tgcggggcta tggcaagacc accttcacgc acgcagagga tgaaagatgc cacctgtaca
     1561 ccctgcatct ggccctggtg atgaaggcgg agtgctacta ccggaattat gagaccgacg
     1621 aggtcagcaa tatcgcaacg caactgatcg ggccggccct gacctggctg cgggagaact
     1681 gaccgccaat gcctgccccc gcaagggtgg aacgcctggg cacggcgcca gtcgggaagc
     1741 tgctcctgga aatgtcgtcc cagaccacgt tttcgctgct ggtctatgcc gtctacagcg
     1801 tcaccgacac ctacttcctg tcggtgggca tcaactcgct ggctgctgcc ggggcctcga
     1861 tcatcgcgcc cgtattgacg gccctcggag gggttgccac cacggtgggg gccgggggcg
     1921 cctcggtggt gtcgcgggcg cttggcgagg cgaacccgca gaaggcgtcc cggacggtcg
     1981 ccaatacctt cctgatcttc tgggtcgtgg cgcttgcgat cacggtgggc ggtgcgctgt
     2041 ttatccagcc catcgtctac ctgctcggcg cgacggacag cgtggcgcct tatgccgttg
     2101 agtatggccg gattatcttc ctcggcgcgc tcaccagcac cgggtactcc gccatcgtcc
     2161 gggccgacgg caatatcgga tactcgacgg cgatgtggat cgttcccgtg actgccaata
     2221 cggtgttgtg ctggctgttc gtcatcgtcc tgcatatggg cgttgccggg gcggccctgg
     2281 cgaccgtcct ggggcaggcg atctctgccg gcatgagcat ctatttcttc ttcttgcgca
     2341 agaaccggtc atatcagatc aaggcggcgt atttccaacc cgattgggcg atcatgggcg
     2401 aggtcctcat catcgggttt ccctcgttcg tcaaaggcct cagcaccggc ctggtcggca
     2461 tcgtcaccaa caacctgcta cgggtgatcg gcggcgatag cgcgctgggc gtgtttgcca
     2521 tcgtcaaccg gctctatttc gcgctgagca cgccgcagtt cggtatcatg caggggatgc
     2581 agccgatcct gggctataac ttcggccgga agcagtttgg ccgggtgcgg caaaccatca
     2641 cctattcact cggcacggcg gtcgtatacg ggctgattgt ctgtagtttg tgcctgctga
     2701 ttccaacgac gctagtcgcg ctgctgtcca aggaacccag gattgtcgcc gaaggccagg
     2761 acgccttacg attaatggcg ttggcctacc cgtttgccgg gatttcgctg atggtcgccg
     2821 cgtattttca agcggtgggc cgggccaagg aagcattact gatcacactg gggggcatcg
     2881 ttctcgtcaa actgccggtg ctgttgctgg catccggcct gttctccttg acgggaattt
     2941 gggccgcgga ggcggcctcg gaattcattt tatgcgtcgg ttccttgctg atgttgcgga
     3001 gttaccagga gaagatggtc acaatggagc gtctggctta cagtccctga tcgggcaggg
     3061 gtgattcacc gttgaaaagc gcccgtctga tcagcaggcg tttaatcggc tgcgctggaa
     3121 caagctgcca accggcggct tgttttgctg tttgtcgccc ggacccttgg gtgcgtatcc
     3181 ggtgtcgtcg cgggttcggg ggggtggccg gagaccggcc tatcgaaccc gcgacgcatc
     3241 tgctggttgc gtgaagcaga agatatgcca ccacaaaacg tggtgatagc gtgagagaac
     3301 tttgaaactg ccttgctcta gccggaatcg ctgctccatt acttgagcat ttttgtgtta
     3361 actgcgggcg atctgagcct cttgatctct tatgttaatg gctgtactat aagaatacaa
     3421 ccacgcgccg gggtggcaaa ttgggcgcaa ggagggacct ggaaacatgc ctgatgagga
     3481 acagatcagc cggctggctc aactggggct gaacgcctac gaggcgcggg cctacctggc
     3541 gctgctgggc aaggaaagct tcagcgcgtc ccaggtggcg gacctctctg gggtgccgcg
     3601 ccagcgcatc tacgatattc tggcgagcct ggtggagcag gggctggcgg tgagccggcc
     3661 cggccgccgg ggcaccaagt atgccgccgt ggcccccggc gtggcgttgc aggcgctgct
     3721 gggccgcgcc aagcagcagg tgacggcgct ggaagtggcg accaacagcc tgatcacgac
     3781 cctggcctcg caatatgccg cgggcaaggg cgagacgagt ccgctcgaat acatcgaggt
     3841 gctgcgaggg acggcggcga tcaaccagcg cttcgccgag atccaggcca attgccgccg
     3901 cgagatcctg gtcttcacca agccgccgta tgctaccccg ccccaggaga acgtcgaagg
     3961 gctggagacg ctccaccgcc atgtgcgcgc ctgctcgatc tacgagtacg acgtgttgcg
     4021 cgacccggcc acgcgcgacg gcgttgccta cttcctgcaa cagggcgagc aggcccgctt
     4081 tgtcgcccac ctgccgatga agctggtagt cgtcgacgaa gccatcgtca tgttcgccat
     4141 ggaggacccc atcgccgggc gcaccgatct gacgatcatg gtcatcgagc acccgcaact
     4201 ggccgcgg
//
LOCUS       DASUYC010001188         6508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_343358,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001188 DASUYC010000000
VERSION     DASUYC010001188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 6508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6508
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343358"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VF276_19410"
     CDS             complement(<1..467)
                     /locus_tag="VF276_19410"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6609078.1"
                     /translation="MTLYQEVFSTAHCGPAPARALQGRATEQDLLLWALSSQRTVKAQ
                     DGTGAEFALIERRLPAGFAAPAHIHHGGDEAFYVLEGEMRFQCGDQAITGTPGAFIYL
                     PRQVPHTFRVGPAPARILQFHTARGDAGLAPGPLSMARMLALTGTYPIELLLPP"
     gene            complement(507..683)
                     /locus_tag="VF276_19415"
     CDS             complement(507..683)
                     /locus_tag="VF276_19415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6609079.1"
                     /translation="MSGTSIYQLEQQMHDTTKQIAAELDHLRHAGQARCGDQRRAART
                     LWQIGRLRFVWAPR"
     gene            977..1663
                     /gene="nth"
                     /locus_tag="VF276_19420"
     CDS             977..1663
                     /gene="nth"
                     /locus_tag="VF276_19420"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX6609080.1"
                     /translation="MNDGDGLEPAEKINPIYHALLRVYGQPTWRPHHPPLDELVLTIL
                     SQHTSDLNSGRAFEQLRAALPTWEAVRDAPVSAIAAAIQSGGLAQMKAPRIKAILQRI
                     WDERGSFDLDFLKTVPLDEARAWLAALPGVGPKTAACVLLFACGRPAMPVDTHVHRVS
                     RRLGLVPDRATAEQAHVLLEALVPPEWYYAFHLNVIKHGREVCKAPRPRCEICVLTQW
                     CDYYRGVRKA"
     gene            1660..2385
                     /locus_tag="VF276_19425"
     CDS             1660..2385
                     /locus_tag="VF276_19425"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX6609081.1"
                     /translation="MNLYLVRHGLSTWNVSGRLQGWANTDLTPEGFEQAQRTAEFFSD
                     FAQRQRMRFYALYSSPLLRAWLTAHAIGDRLGLVPIPVDDLREMQGGAVEGLRREEWQ
                     RRYPELIPGWSDRKNLDFGWPGGETRRAFRNRCMRAISEIVAQHKSYDNIIVVTHGGV
                     IKAYLNSAGLDDPMGPRSYEADNCSVTHIQFMGSDTADDRGGEIWSVGCLRIFNQVEH
                     LRDEDSDVDDELVATARADLALQ"
     gene            2418..3113
                     /locus_tag="VF276_19430"
     CDS             2418..3113
                     /locus_tag="VF276_19430"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX6609082.1"
                     /translation="MYLFLIRHGQSEGNVAGRLQGWQDAPLTPLGEHQASRAAMALAT
                     FLQSAGVSVATVYSSPLQRARRTADVISQAIGAPLEIDPGLREMHFGRVEGLTDAEWK
                     DCFPELLADWHRHDNLDFGWPDGETRRAFYTRIEQAIDGIVASQPPGANPVIVAHGGV
                     ISSYLSHLTTGEWHHWRRFSVGNCSITHVTLEPDRPAPPAMSCLLRFNETSHLDDPDV
                     EAVTGEEDAGSAA"
     gene            complement(3256..3777)
                     /locus_tag="VF276_19435"
     CDS             complement(3256..3777)
                     /locus_tag="VF276_19435"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEX6609083.1"
                     /translation="MNWVDVTILTAIVVSALLGLFWGLIRQIAATFGLILAIFLAGAN
                     YKAVAAFLHPPQGGGLIADENLANIIAFVLILVGVSLAVGIVASVLRTALGLLFLGWA
                     DHLLGALLGVFQMLLLMAVIIMVATLFPVPGLSDAVRQSTLAPAVARPFAFVLDWLPP
                     EFGLLRFLLNTGG"
     gene            complement(3774..4751)
                     /locus_tag="VF276_19440"
     CDS             complement(3774..4751)
                     /locus_tag="VF276_19440"
                     /inference="COORDINATES: protein motif:HMM:NF020660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HEX6609084.1"
                     /translation="MQTNETEPSQVRCTEETAQGRAILVLENGESRLAFDPAQGGRWT
                     TWDLALEGGPVHHVMSAPGTVISAGPARLAAVGQTPEVMASLGPATPSGLGDHFLPLT
                     VRQRDFALGTARELGTFLAQPFEATHYAPARDQQTVALRSAGGIQGAKRVTPVTLLKR
                     LTLGRPGGEISIHYRLDNPNEKALQIFFGVEFCFALSPEAVAAGADHPAYEFDGARER
                     GGYGVRGIAADTTSVALIDPQPGLTIRLGWERPAAVWICPSPAGAEGPAIKGASVMPV
                     WDLRVPPDDNWAVNIWLQPGATGPVAPIPADVVARIARFESEDTPWKQP"
     gene            5010..6359
                     /gene="glp"
                     /locus_tag="VF276_19445"
     CDS             5010..6359
                     /gene="glp"
                     /locus_tag="VF276_19445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX6609085.1"
                     /translation="MSATKMGSITVGEALAYILSHFAPLPPEPTPLAEADGRVLAEDV
                     TSDIDIPPFANSAMDGYAVRAADTQGATRAAPRPLRVIGNVAAGYVAGATVGPGEALR
                     IMTGAPLPGGADAVIRFEETSEGDALHAGAAHAGNRPPEGAKAAWRDVAGSNAASGAT
                     EVQLYAGVQTWDNVRHAGEDVRRGQVVLARGTVIRPFEVALLAAVGRPQVLTHRRPRV
                     AILSTGDELVDVDQPVGPGQIRNSNSYGLAAQVRAAGGVPILLGIARDNLAELTARVE
                     EGLAQAPDLFVTSAGVSVGDYDIVKDVLDREGTMHFWQVRMKPGKPLAFGVVRGVPLL
                     GLPGNPVSSLVSMEQFGRPAILKMLGRPHLGRPVVTVRALEAIPNKSGREHYIRAVVT
                     RNAAGEYEARSTGEQGSGILTSLTGANAMLIVEETRTLVQPGETVRAIMLDWPEQVF"
BASE COUNT         1070 a         2181 c         2251 g         1006 t
ORIGIN      
        1 ggcggcagca gtaattcgat ggggtacgtt cccgtgagcg ccagcatgcg ggccatgctg
       61 agcggacccg gcgctaggcc cgcgtcccca cgggcggtat ggaactggag tatgcgggcg
      121 ggcgccgggc ccacccggaa ggtatgcggc acctggcgcg gcaggtaaat gaacgcgccc
      181 ggcgtgcccg tgatcgcctg gtcgccgcac tggaagcgca tctcgccctc cagcacatag
      241 aatgcctcgt cgccgccatg atgaatatgc gcgggcgcgg caaagcccgc gggcagccgc
      301 cgctcgatca gcgcgaattc cgcgccggtg ccatcttgcg ccttgaccgt ccgctggctg
      361 ctcagcgccc agagcagcaa atcttgctcg gtggcccggc cctgcagggc gcgggcgggg
      421 gccggtccgc agtgcgccgt cgaaaacact tcctggtaca acgtcatctt cgtccctcta
      481 aaacgaatct ccgagtcccg cgccgattaa cgcggcgccc agacgaagcg caggcggccg
      541 atctgccaca gcgtgcgcgc cgcgcgccgc tggtcgccgc agcgggcctg cccagcatgg
      601 cggaggtgat cgagttcggc agcgatctgc ttcgtcgtgt cgtgcatctg ctgctccaat
      661 tgatagatac ttgtgccgct catgagtttg ctcctgctgc cacctggcaa tactgatcgt
      721 gctcgttgtg cgtccggcgt tgccgggcgg gatggtgttg gctgaatatc ttgctgagtc
      781 tattaaagca caggatggcc taaactgatc ctgacaaaaa aagcgacaaa agcctgacat
      841 ctagccatcc ggcgggggcc cggcgccggg gacgcagcgc cgcggggcac gaatcgcata
      901 tttcgggtat aatagtgcta tgcgtgttca cagtcacgaa gtaaactaca cctcccccgc
      961 gcccggtgcg gcgaccatga atgatggcga tgggctggaa cccgccgaga agatcaatcc
     1021 gatctaccac gcgctgctgc gggtctacgg gcaaccgacc tggcgcccgc accatccgcc
     1081 gcttgacgaa ctggtgctga ccatcctttc gcagcacacg tcggatctga acagcgggcg
     1141 ggccttcgag caattgcgcg cggcgctgcc cacatgggag gccgtgcgcg atgccccggt
     1201 gagcgccatt gccgctgcga tccagagcgg cggcctggcc cagatgaaag cgccgcgcat
     1261 caaggccatc ttgcagcgca tctgggacga gcgggggagt ttcgacctcg acttcttgaa
     1321 gactgtccct ctggatgagg cgcgcgcctg gctggcggcg ctacccggcg tagggccgaa
     1381 gacggcggcg tgcgtgctgc tgttcgcctg cggccggccc gccatgcccg tggacaccca
     1441 tgtccaccgg gtcagccgcc gcctgggcct ggtgcccgac cgggcgacgg cggagcaggc
     1501 gcacgtgctg ctcgaagcgc tggtgccgcc ggaatggtat tatgctttcc acctgaacgt
     1561 catcaagcat ggccgcgagg tgtgcaaggc accccgcccg cgctgcgaga tctgcgtcct
     1621 cacccagtgg tgcgactact ataggggggt acgcaaggca tgaatctcta cctggtccgc
     1681 catggactct ccacctggaa cgtttcgggc cgcctgcagg gctgggcgaa tacggatctg
     1741 acgcctgaag gcttcgagca ggcccagcgc accgcggagt tcttctccga tttcgcccag
     1801 cgccagcgca tgcgtttcta cgcgctctac agcagcccgc tgctgcgcgc ctggctcact
     1861 gcccacgcca tcggcgatcg cctgggactg gtgcccattc ccgtcgacga tctgcgcgag
     1921 atgcagggcg gcgcggtcga agggctgcgc cgcgaggaat ggcagcgccg ttaccctgaa
     1981 ctgatccccg gttggagcga ccgtaaaaat ctcgattttg gctggccggg tggggagacg
     2041 cggcgagcct tccgcaaccg ctgcatgcgg gccatttccg agatcgtggc ccagcataaa
     2101 tcgtacgaca atattattgt ggtcacgcac ggcggggtca tcaaagccta cctcaacagc
     2161 gccgggttgg acgacccgat gggaccgcgc tcctacgagg cggacaattg cagcgtcacc
     2221 cacatccagt tcatggggtc ggataccgcc gacgacaggg gcggcgagat ctggtcggtg
     2281 ggctgtttgc gcatattcaa ccaggtggag cacttgcgcg acgaggacag cgacgtagac
     2341 gatgagctgg tcgcgacagc gcgcgcggat ctggccctgc aatagcgcgc gctaccgcta
     2401 cgggcggaag gagccgtatg tacctctttt tgatccgcca cgggcaatcc gagggtaatg
     2461 tcgccgggcg cttgcagggc tggcaagatg cgccgctcac gccgctgggc gagcatcagg
     2521 ctagccgtgc ggccatggcc ctggcgacct tcctgcagtc cgccggggtc tcggtcgcca
     2581 cggtgtacag cagccccttg cagcgcgccc ggcgcaccgc cgacgtgatc agccaggcca
     2641 ttggggcgcc gctggagatc gatccgggac tgcgcgagat gcatttcggg cgggtcgagg
     2701 gcctgaccga cgccgaatgg aaggattgtt tccccgagct gctggcggac tggcaccggc
     2761 acgacaatct cgacttcggc tggccggacg gtgaaacgcg ccgcgcgttt tacacgcgca
     2821 ttgagcaggc catcgacggg atcgtggcct cccagccgcc gggcgcgaac ccggtcatcg
     2881 tggcccacgg cggcgtgatc tccagctacc tgtcgcacct caccacgggc gaatggcacc
     2941 actggcggcg tttctcggtc ggcaattgca gcatcaccca tgtgaccctg gagccggacc
     3001 ggcccgcccc gcccgccatg tcctgcctgc tgcgcttcaa cgaaaccagc cacctggacg
     3061 accctgatgt ggaggctgtg accggcgaag aggacgccgg cagcgcggcc tagtagccgg
     3121 cgcgaccctg cttttgcgac ggggatggga ccggcttcaa accggctggg ctcctgtaca
     3181 aaccgatccg ctatggcgct gtaggggccg gttttaaacc ggcctgccgc ccgtcagaat
     3241 ctgtgcgccg ggctactaac cgcccgtatt caacaggaag cgcagcaacc cgaactccgg
     3301 cggcagccag tccagtacga aggcgaacgg gcgggccacc gcaggggcca gggtcgattg
     3361 gcgcacggcg tccgacaggc caggcacggg gaagagcgtc gccaccatga tgatcacggc
     3421 catcaacagc agcatctgga acacgcccag cagcgccccg aggagatgat cggcccagcc
     3481 gaggaacagc aaacccagcg ccgtgcgcag caccgaggcc acgataccca cggccaggct
     3541 cacgccgacc aggatcagga caaaggcgat gatgttcgcc aggttctcgt ctgctatcag
     3601 cccgccgccc tgcggagggt ggaggaaggc ggccacggcc ttgtagttgg ccccggccag
     3661 gaagatagcg aggatcagcc cgaaggtcgc cgcgatctgg cggatcaggc cccagaacag
     3721 gccgagcagg gcgctgacga caatggccgt taggatcgtt acgtcgaccc agttcatggc
     3781 tgcttccagg gggtgtcttc ggactcgaat cgggcgatgc gcgccaccac gtcggcggga
     3841 atgggggcca cggggccggt ggcgccgggt tgcagccaga tgttgaccgc ccagttatcg
     3901 tccggcggca cgcgcagatc ccacaccggc atgacgctgg cacccttaat cgccggccct
     3961 tcggcgcccg cggggctggg gcagatccag accgccgcgg gccgctccca gcccaggcgg
     4021 atggtcaggc cgggttgcgg atcgatgagc gccacgctgg tggtgtcggc ggcgatgccg
     4081 cgcacgccgt agccgccgcg ttcgcgcgcc ccgtcgaact cgtaggcggg gtgatcggcg
     4141 cccgctgcca cggcctccgg gctgagagcg aaacagaact cgacgccgaa gaagatttgc
     4201 agcgccttct cgttggggtt gtcgagccgg tagtggatgc tgatctcgcc gccgggccgg
     4261 cccagcgtca gccgcttgag cagcgtgacc ggcgtcaccc gcttggcgcc ctggatgccg
     4321 cccgcgctgc gcagggccac ggtttgctgg tcgcgggccg gggcgtagtg cgtggcctcg
     4381 aacggttggg ccaggaaggt gcctagttcg cgggccgtgc cgagcgcgaa gtcgcgctgg
     4441 cgcaccgtga gcggcaggaa gtggtcgccc aggcccgacg gcgtggcggg gccgaggctc
     4501 gccatgacct ccggcgtctg gcccacggcg gccagccggg ccgggccggc gctgatgacc
     4561 gtgccgggcg cgctcatcac gtggtggacc gggccaccct ccagcgccag gtcccaggtg
     4621 gtccagcgcc cgccctgcgc cgggtcgaac gccaggcgcg actcgccgtt ctccaggacc
     4681 aggatggccc ggccctgcgc cgtctcttcc gtacagcgca cctgggatgg ctcggtctcg
     4741 ttcgtctgca taaaaccagt ctaccatacg cgctgaggag cgtcaacgcg cccggcgcag
     4801 ccgtccgtaa actcaccgcg aaggcgcgaa gagcacgaag atcctcatta ttaatctcgt
     4861 tcttcgcgtc cttcgcgcct tcgcggtaaa attgcacgtc tggcgggcgg tacggagctt
     4921 gcagcggcaa gagcatccgg ttttgcgccg ggtgcgtaga taccgattga acagcacatc
     4981 cctgggaaga tcagcggagg aggccaaacg tgagcgcaac aaagatggga agcattaccg
     5041 tgggcgaggc cctcgcttat atcctgagcc atttcgcgcc gctgccgccg gaacccacgc
     5101 cgctcgccga ggccgatggg cgcgtgctgg cggaggacgt gacctccgac atcgacatcc
     5161 cgccgttcgc caactcggcc atggacggct acgccgtgcg cgccgccgat acgcagggcg
     5221 ctacccgcgc cgcgccgcgg cccctgcgcg tgatcggcaa tgtcgcggcg ggctacgtcg
     5281 ccggggcgac ggtggggccg ggcgaggcgc tgcgcatcat gaccggcgcc ccgctgcccg
     5341 gcggcgccga cgccgtgatc cgcttcgagg agacgagcga gggcgatgcc ctacacgcgg
     5401 gcgccgcgca cgccggcaac cgcccgccgg agggcgcgaa agcggcctgg cgcgacgtgg
     5461 ccgggagcaa cgcggcgtcc ggcgcgaccg aggtgcaact ctatgccggg gtgcagacct
     5521 gggacaatgt gcgccacgcg ggcgaggatg tgcggcgggg ccaggtggtg ctggcgcgcg
     5581 gtacggtgat ccggcccttc gaggtagcgc tgctggccgc cgtggggcgc ccgcaggtgc
     5641 tcacccatcg ccgcccgcgc gtggcgatcc tctccaccgg cgacgaactg gtggatgtgg
     5701 accagccggt ggggccgggc cagatccgca acagcaacag ctacggcctg gccgcccagg
     5761 tgcgcgcggc gggcggcgtg cccatcctgc tcggcatcgc ccgcgacaac ctggcggaac
     5821 tgacggcgcg cgtggaggag gggctggccc aggcgcccga tctattcgtc acctcggcgg
     5881 gcgtctccgt cggcgactac gacatcgtga aggacgtgct cgaccgcgaa ggcacgatgc
     5941 acttctggca ggtgcgcatg aagccgggca agccgctggc cttcggcgtg gtgcgcggcg
     6001 tgccgctgct ggggctgccc ggcaaccctg tctcctcgct ggtgagcatg gagcagtttg
     6061 gccgcccggc tattctcaag atgctgggcc gcccgcacct gggacggccc gtggtcacgg
     6121 tgcgcgcgct ggaggcgatc cccaacaagt cgggacgcga gcactacatc cgcgccgtgg
     6181 tcacgcgcaa cgccgccggc gagtacgagg cgcgcagcac cggcgagcag gggtcgggca
     6241 tcctgacctc gctgacaggg gccaatgcca tgctcatcgt cgaggaaacg cgcacgctcg
     6301 tccagccggg cgagacggtg cgcgccatta tgctggactg gccggagcag gtcttctgat
     6361 cgcgcgccgc ttcccttggc cgccggttgg ccccgcccac cgccaacatc cgggtagggc
     6421 ggcaggcgcg ataaatcgcg ccgctacgaa ttacgcctga ataaaggcgc tgacgggcaa
     6481 catgcttgtc tgattcgtag tggcgcga
//
LOCUS       DASUYC010001189         2545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1145086, whole genome shotgun sequence.
ACCESSION   DASUYC010001189 DASUYC010000000
VERSION     DASUYC010001189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145086"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1207)
                     /locus_tag="VF276_19450"
     CDS             complement(<1..1207)
                     /locus_tag="VF276_19450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional rhamnulose-1-phosphate
                     aldolase/short-chain dehydrogenase"
                     /protein_id="HEX6609086.1"
                     /translation="MVMESKTYRHVTYAWDDAVAAGLDPLGRLVYRSNLLGADGRITN
                     TGGGNTSAKLTETDPLTGKPVSVLWVKGSGGDLRTAKRENFSSLYMDKLIALQQHYHH
                     APHRGPKTEIEDAIVGLYAHCTFNLNPRPSSIDTPLHGFVPYRHVDHTHPNAVIAVAA
                     ARNSERLTREIYGDDVIWTAWQRPGFDLGLKLEQIVRDHPRARGVVLGGHGLINWADD
                     DKACYELSLDLIERAGRYIEEHDKGAQTFGGARYQALVENQRHAGLIEILPWLRGQVS
                     QYKRMVGTVQEDPAILRFVNSHDAPRLAELGTSCPDHFLRTKIKPLYVNWDPQNEDMA
                     ALKQKLRAGLEQYRRDYARYYEQHKHPDSPAMRDTNPTVILIPGLGMIAWGKDKSESR
                     VTAEFYNCAV"
     gene            complement(1222..2502)
                     /locus_tag="VF276_19455"
     CDS             complement(1222..2502)
                     /locus_tag="VF276_19455"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HEX6609087.1"
                     /translation="MNSTSPTTKQVQDAYTLARDRYAALGVDTDQALAALDKVALSLH
                     CWQGDDVAGFESPDAALGGGLAATGNYPGKARTAAELRRDLDRTYSLIPGTHRLNLHA
                     IYAETGGQKVERDALQPEHFQGWVEWAKTHGHGLDFNPTLFSHPLAESGFTLASPDPA
                     IRRFWIDHCIACRHIGAYFGRELGTACATNIWIPDGLKDTPVDRGAPRRRLQESLDAI
                     LAVPIDAQYNLDSVESKLFGLGSESYVVGSHEFYLGYAIHKQILLTLDSGHFHPTESI
                     ADKISAVLLYVPGLVLHISRGVRWDSDHVVTLNDDTQAMLREVVTGGYLDRVHIGLDF
                     FDASINRIAAWTIGARNVLRAALLALLEPTARLREMEESGDYTARLAWQEALKGLPWA
                     AVWDYYCTQHDVPTGLGFLDEVRTYEAQELSKRA"
BASE COUNT          372 a          778 c          912 g          483 t
ORIGIN      
        1 cgaccgcgca gttgtagaac tcggcggtca cgcgcgactc gcttttgtcc ttgccccagg
       61 cgatcatgcc caggccgggg atcaggatga cggtggggtt ggtgtcgcgc atggcgggcg
      121 agtcgggatg cttgtgctgc tcgtagtagc gggcgtagtc ccggcggtat tgttccagcc
      181 cggcgcgtag cttttgcttc agcgccgcca tatcctcgtt ttgcggatcc cagttgacat
      241 aaagcggctt gatcttggtg cgcaggaagt ggtcggggca ggaggtgccg agttcggcca
      301 ggcgcggggc gtcgtggctg ttcacgaagc gcaggatggc cgggtcttcc tgcaccgtgc
      361 cgaccatgcg cttgtactgg ctgacctggc cgcgcagcca gggcaggatc tcgatcagcc
      421 cggcatggcg ctggttctcg accagcgcct ggtagcgggc gccgccgaag gtctgcgcgc
      481 ctttgtcatg ctcctcgata tagcggccgg cgcgctcgat caggtcgagg ctcagttcgt
      541 agcaggcttt gtcgtcgtcg gcccagttga tcaggccgtg cccgcccagg accacgcctc
      601 tagcccgcgg gtgatcccgc acgatctgct ccagcttcag ccccaggtcg aagccgggcc
      661 gctgccacgc cgtccagatc acgtcgtcgc cgtagatctc gcgcgtcagg cgctccgagt
      721 tgcgcgccgc cgccaccgcg atcaccgcgt tggggtgggt gtgatcgaca tgccggtagg
      781 gcacgaagcc gtgcagcggc gtgtcgatgg acgaggggcg ggggttcagg ttgaaggtgc
      841 agtgggcgta cagcccgacg atggcgtcct cgatctcggt cttggggccg cgatgaggcg
      901 cgtggtggta gtgctgctgt agcgcgatca gcttatccat gtagagcgag gagaagttct
      961 cgcgcttggc ggtgcgcagg tcgccgccgg agcctttgac ccagagcacg ctcaccggtt
     1021 tgccggtcaa cgggtcggtt tcggtgagct tggccgaggt gttgcccccg ccggtattcg
     1081 tgatgcgccc atcggcgccc agcaggttgg agcgatagac gagccgcccc agcggatcca
     1141 gcccggcggc gacggcatcg tcccaggcgt aggttacgtg gcggtaggtt ttggattcca
     1201 tcaccatcgc ggggttcctc cttaggcgcg cttgcttagt tcctgggcct cgtaggtccg
     1261 cacttcgtcc aggaacccca ggccggtagg cacatcgtgt tgggtgcagt agtaatccca
     1321 gaccgccgcc cagggcaggc ccttgagcgc ctcctgccag gccaggcgcg cggtgtagtc
     1381 gccggactcc tccatctcgc gcaggcgggc ggtgggttcg agcagggcca gcagggccgc
     1441 gcgcagcacg ttgcgcgccc cgatagtcca ggcggcaatg cggttgatgc tggcgtcgaa
     1501 gaaatcgagc ccgatatgca cccggtcgag atagcccccc gtcacgacct cgcgcagcat
     1561 ggcctgggtg tcgtcgttca gcgtgaccac gtggtcgcta tcccagcgca cgccgcggct
     1621 gatgtgcagc accaggcccg gcacgtacag cagcaccgcc gatattttgt cggcaattga
     1681 ttccgtggga tggaagtggc cgctgtccag cgtcagcaag atctgcttgt ggatggcgta
     1741 gcccaggtag aattcgtgcg agccgaccac gtagctctcc gaaccgaggc caaacagctt
     1801 gctctcaacc gagtccaggt tgtactgtgc gtcgatgggc acggccagga tggcgtccag
     1861 cgactcctgg aggcggcgac gaggcgcgcc gcggtcgacg ggggtgtctt tgaggccgtc
     1921 ggggatccag atgttggtgg cgcaagcggt gcccagttcc cgcccgaaat aggcgccgat
     1981 atggcggcag gcgatacaat ggtcgatcca gaaccggcgg atggccgggt cggggctggc
     2041 gagcgtaaag ccgctctcgg ccagcgggtg ggagaaaagg gtggggttga agtcgagccc
     2101 gtgcccgtgt gttttcgccc actcgaccca gccctggaag tgctcgggct ggagtgcgtc
     2161 gcgctcgacc ttctggccgc cggtttcggc gtagatagcg tggaggttga gccggtgggt
     2221 gccggggatc aggctgtagg tgcggtcgag atcgcggcgc aactcggcgg cggtgcgggc
     2281 cttgccgggg tagttgccgg tagccgccag gccgccgccc agggccgcgt ccggcgactc
     2341 gaatccggcc acgtcgtcgc cctgccagca gtggaggctc aaggcgactt tgtcgagtgc
     2401 ggctaacgcc tggtccgtat ccacccccag ggcggcatag cggtcgcggg ccagcgtgta
     2461 cgcgtcttgc acctgcttcg ttgtcgggga cgtgctgttc atccgttgtc cttcctgcta
     2521 tcagtcgaga tggaaaatct cggtt
//
LOCUS       DASUYC010001190         2410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_2073689, whole genome shotgun sequence.
ACCESSION   DASUYC010001190 DASUYC010000000
VERSION     DASUYC010001190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2073689"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VF276_19460"
     CDS             <1..296
                     /locus_tag="VF276_19460"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional nuclease domain-containing protein"
                     /protein_id="HEX6609088.1"
                     /translation="VLDVNGRHAEIDCRSSDAVAIAVRVHSRIYVDESVMERGGVTLD
                     GEPATAEASADHPEAAGSEGPKPDDERLSVFRDFINSLDIDDIDRPGRDENRG"
     gene            complement(413..1354)
                     /locus_tag="VF276_19465"
     CDS             complement(413..1354)
                     /locus_tag="VF276_19465"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HEX6609089.1"
                     /translation="MPVLEYDPQQFSLDDTLACGQAFRWERLPDGAWRGVVRDAVITL
                     RQDGARLHYTATTGDVAPLLRDLFLLDEDLPAIQAALIAVDPALAGPIGRAPGLRRMR
                     QDPEECLLSFICSTANQVPRIVAGIRHLSAALGPALGTVEGRTYHAFPTAATLAAADP
                     ARLQAATGLGYRAGRLVDVGRQLVARGSEWLPGLRAAPYAAAHNALVALHGVGPKVAD
                     CVCLFSLDHTEAVPVDTHIWQMAQQLWGRDQSGVPLAPGETPRVQGLTAAAYRRVGDL
                     FRARYGALAGYAQIWLFYDHFRGYWGGTSPLLRAAGG"
     gene            complement(1361..>2410)
                     /locus_tag="VF276_19470"
     CDS             complement(1361..>2410)
                     /locus_tag="VF276_19470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6609090.1"
                     /translation="FAAPDGTAVLDGVMRSFQPAAAPPPRGGYAPLLFLRNGNLWEAD
                     SAGGSLRQVTTGGRVRGFALSSDLTHLALLLAGSAAAHTAETLEVRTLTGSGATALWH
                     TDEIQAVAWYGEHELVALAKEAGMRGLWRMAATKDGTRRLLANFKSLPDGGAQAGSLH
                     VAPDRLWITFLAGSRDGQDLYGMRPDGSGLRSLLPNTAQRPAVSQYAWMPPDSATGAA
                     WILLLADRQLQRLTLPAAGAIESDLIPLATLPNDQFDDLVVAPAGQAAALTHPGDAPP
                     QVCVGADPLAGESYHLIVLEPGQFVPGSLTWAPNGQYLIYREAAANGAERVLQVDTAG
                     SSIAPPIDLPLEPAR"
BASE COUNT          357 a          855 c          872 g          326 t
ORIGIN      
        1 tggtgctcga tgtcaacggc cggcacgccg agatcgactg ccggtcgagc gacgccgtgg
       61 ctatcgccgt gcgcgtgcac agccgcatct atgtcgatga gagcgtcatg gagcgcggcg
      121 gtgtgaccct ggatggcgaa ccggcgactg cggaagcaag cgccgaccat ccggaggcag
      181 caggcagcga aggtcccaag cccgacgatg agcggctgtc ggtcttccgg gattttatca
      241 acagcctgga catcgacgac atcgatcggc ccggtcgcga cgagaatcgc ggttaggccg
      301 gccggcggcg cctcctgact tctacaactc acgcactgca acacgcctcc ggccgcccgg
      361 ctggaggcgt ttgctgtttt agcggggttg cgaccctgcc caccgggccc atttatccgc
      421 ccgccgcccg gagcagcggg ctggtgccgc cccagtagcc ccggaagtgg tcgtagaaca
      481 gccagatctg ggcgtagcct gccagggcgc cgtagcgggc gcggaacagg tcgcccacgc
      541 ggcggtaggc cgccgccgtc agcccctgca cgcgcggcgt ttcgccgggc gccagcggca
      601 ccccggactg gtcgcgaccc cagagctgct gggccatttg ccagatgtgc gtatccaccg
      661 gcaccgcctc ggtgtggtcg aggctgaaga ggcagacgca atcggccact ttggggccca
      721 caccgtgcag cgccaccagc gcgttgtgcg ccgccgcgta gggcgccgcc cgcaggccgg
      781 gcagccactc ggatccccgc gccaccagtt gccgccccac gtccaccagc cgccccgccc
      841 ggtagcccag ccccgtcgcc gcctgcaagc gcgcgggatc ggcggcggcc agcgtcgcag
      901 cggtggggaa ggcgtgatag gtccggcctt cgaccgtgcc gagggccgga cccagcgccg
      961 cgctcaggtg gcgaatgccc gcgacgatgc gcggcacctg gttggcggtg gagcagatga
     1021 acgagagcag gcactcctcg gggtcctggc gcatccggcg caggccgggc gcccgcccga
     1081 tcgggccggc cagtgcgggg tcgacggcga tcagcgccgc ctggatggcg ggcaggtcct
     1141 cgtccagcag aaacaggtcg cgcagcagcg gcgcaacatc gcccgtcgta gcggtatagt
     1201 gcaggcgggc gccgtcctgg cgcagggtga tcaccgcgtc gcgcaccacg ccgcgccagg
     1261 cgccgtccgg caggcgctcc cagcggaacg cctgcccgca ggccagggta tcgtctaacg
     1321 agaactgctg gggatcgtat tcgagaaccg gcatgcgcgg ctagcgcgcc ggttccagcg
     1381 gcaagtcgat gggcggggcg atgctgcttc ccgccgtgtc tacctgcaac acccgttcgg
     1441 cgccgttcgc ggcagcctcg cggtaaatca ggtactgccc gttgggcgcc caggtcaggc
     1501 tgcccggaac aaactggccc ggctccagca cgatcaggtg ataggactcg ccggccagcg
     1561 gatcagcccc cacgcagacc tgtggcggcg cgtcgccggg gtgggtgagc gccgcggcct
     1621 gcccggccgg ggccaccacc aggtcgtcga actggtcatt gggcagcgtc gccagcggga
     1681 tcagatcgga ctctatggcg ccggctgccg gtaaggtgag gcgctgcaac tgccggtcgg
     1741 ccagcagcag gatccaggcg gcgccggtgg cgctgtcggg cggcatccag gcatactggc
     1801 tgacggccgg gcgctgcgcg gtgttcggca gcaacgagcg cagcccgctg ccgtcggggc
     1861 gcatgccgta gagatcctgc ccgtcccggc tcccggccag gaaggtgatc cagagccgat
     1921 cgggggcgac atgcaggctg cccgcctgcg cgccgccgtc gggcaggctc ttaaagttcg
     1981 ccagcaagcg gcgggtgccg tccttggtcg ccgccatccg ccacagcccg cgcatgcccg
     2041 cttctttcgc cagggccacc agttcatgct cgccgtacca ggccacagcc tggatctcgt
     2101 cggtgtgcca gagcgcggtg gcgcccgaac cggtcagggt gcgcacttcc agcgtctcag
     2161 cggtatgcgc ggcagcggat ccagccagca gcagcgccag atgcgtcagg tcgctcgaca
     2221 acgcgaagcc gcgcacgcgg ccgccggtgg tgacctggcg caggctaccc cccgcgctat
     2281 cggcttccca gaggttgccg ttacgcagga agagcagcgg cgcgtagccc ccgcgcggcg
     2341 gcggcgcggc ggccggttgg aagctgcgca tgactccgtc gagcacggcg gtgccgtcgg
     2401 gcgcggcgaa
//
LOCUS       DASUYC010001191         8292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_776050,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001191 DASUYC010000000
VERSION     DASUYC010001191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 8292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8292
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776050"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1253
                     /locus_tag="VF276_19475"
     CDS             <1..1253
                     /locus_tag="VF276_19475"
                     /inference="COORDINATES: protein motif:HMM:NF022113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LpqB family beta-propeller domain-containing
                     protein"
                     /protein_id="HEX6609091.1"
                     /translation="SVEVSLDLRAGQGSGTRLAPIVNLQLRDAAGQPAPLGPGPMAYL
                     PMNISGKVTRWVADLSAPTVPGTYHMHILARVAGQPDMEFDLRDPALQVVVATPFQGG
                     LVYSRNGNLWRSDAQGGHARRLTYYTGDGRADSPAWSPDGKRIAYTRSLPAAASDIPN
                     TEIWSIDPDGGSPQVLAPRRPGEDLITPAFGPDGALYFTSDRTVDPATNATPTMDRLT
                     EGVESWSVDRLVPAAPDGKRATVLPAARMPDVSRDGKQIVYISAPDAAAESESFITHT
                     LMLADSDGRNARPLFPPDLYQDVLAPRFSPDGTQVAFAAVTPLEVPSGDFDLLRALGL
                     APLPARANGVPWDIYLAPVAGGQIIRLTRLNADQPVVAWANDGKRLALLDEKGLFMLD
                     LADPASLHHVGPPSSHGQLDWYDR"
     gene            1620..2234
                     /locus_tag="VF276_19480"
     CDS             1620..2234
                     /locus_tag="VF276_19480"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HEX6609092.1"
                     /translation="MRYMSVRRVTGAGLPAVLALAAALAWGGGVAVAHAQLSSSDPAD
                     GSQVTMPPPRIVMQFSEAVDPAASTATVSRMDGTRVDNGDAQVDPANRRTLTVGVPAL
                     LPGGYIVQWHAVTPDDNGVTEGSFTFTVVAEGGGVATPVTAPPSPAGGTGSISGGGPA
                     MPRTGHATDTGPFALPGAALLGLGLVLGGLWLVRRSRAGAATLR"
     gene            2280..4511
                     /locus_tag="VF276_19485"
     CDS             2280..4511
                     /locus_tag="VF276_19485"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEX6609093.1"
                     /translation="MGHLPLAWSRFGLARLGALTAILLLSLIFAPRAAAHARLEQSDP
                     PAGAVLLQAPPMVKLTMSESISPQFSQIQVLDRARRDVTAGPVAVDLRDPATIGVVLQ
                     PGLPGGVYTVLWRAISAVDGHLTLGNFAFTIQAPGETIAPAAPAPVGQTGVAPDDGSS
                     PPPPVWRWIWRALVLIGAAGLLGGPLFSSLILAGAPAADLQPLVPALGRRLARLVLVS
                     AVVVLGGLLADMLYELATVRGTDVPAALGNVAQVGDIVTTTGYGAFWAVKVAAAVALL
                     GYALWRGGTPNRPEWTAAVMNGGFLLAGEALGSHGAAARGLGGLPLGMLGDLLHLLAA
                     AAWVGGLIYLAVVALPVVRRAEPVVAGRALARMVPRFSTLALVSIAVLVVTGLVNLAI
                     HTLDPAALVESDYGRVLLVKHLLFLPLVALAALNHGIVRPRLIALLAPGVREAAPPIR
                     SLGRTIGAQVVLAVAVMICAAGLTLLPPPVSAAGSTPGSLPAPAAPPSGPPAPTPTPV
                     IASASQTVSGIGFDLTAAPSGEGDHFTVAVTRRDLAGPPLTDVLKLQLRVTPQDVDAG
                     STSLEVHRVGAMDPDRQVYTATGQIFTLSGAYQLTAELLRTQGADVRAGFRLVLADDG
                     TLAITPSQVLQALVTTNPSPAISGTVQLNIKIVDGQEQPVPGATLRVTPLMPSHGHVE
                     PTGDAVPVAGQPGMYRLPANLPMGGPWLLLVEVDRPGQPPVKLDGTFNVIDPLATATP
                     ASP"
     gene            4714..5721
                     /locus_tag="VF276_19490"
     CDS             4714..5721
                     /locus_tag="VF276_19490"
                     /inference="COORDINATES: protein motif:HMM:NF014312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HEX6609094.1"
                     /translation="MNRQMQWRTVLVLVMMAGVLAACGGDAPPAAEASRATPQGRIMT
                     ADQGANTVSVIDVATNEVYATIPTGQSPHHVVSSPDGREIWVTLYKENHLQVFDAKTF
                     KELGQVDVGGPSDDLTFAPDGKRLYASMGADNMVAVIDPAARKLVTKVGVGQTPHGVK
                     VRPDGKELYVTNTAENTISIISLEGDPKLEISFRVGADPFEVTFDPTGKTAFVSNFLG
                     DSIAVIDTATRKLTGTIRSGKQPAMLSFVEGTGGTLLWVANTGTQEVWIVDPATRKLV
                     MRIPAGGGAHGVVPTPSGKVFVTNTNDNNVTVIEAASGKVITTLGVGTNPNGLTFVPN
                     T"
     gene            5733..6089
                     /locus_tag="VF276_19495"
                     /pseudo
     CDS             5733..6089
                     /locus_tag="VF276_19495"
                     /inference="COORDINATES: protein motif:HMM:NF024515.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6264..7379)
                     /locus_tag="VF276_19500"
     CDS             complement(6264..7379)
                     /locus_tag="VF276_19500"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEX6609095.1"
                     /translation="MPLRVLTTENKITDGADARRWIEQNVPPGCTKVHDHPLFWLDII
                     EPDPAAIDWLAGYFHFHPLTIEDLRSPNERGKIETYEGYAFIIAHAVFVEGSDMLGGP
                     HHAPDQHAGHAGTPAPRRPWQAVPRPGPANDPICDINSYELHAYLSRNYLITVRDAGL
                     KMVDKIWNEIDNHQDDEDPHPLNHGPDYLLYRILDDIADTYFEALERTADQIDYLEDT
                     VIDRPDRDLLNDVFTVKRNLVTIRRQATPVREGLNVLSNPGNAFVRPENQIYLRDVHN
                     LMITVYELADTQRDSTGGVLDAYLSSVNNNLSTVMKRLTIISTIFLPISFIVGFGGMN
                     FVKFIPYDDPLMFWGLMASLVVVPVLMLLWFYRSRWI"
     gene            complement(7667..8212)
                     /locus_tag="VF276_19505"
     CDS             complement(7667..8212)
                     /locus_tag="VF276_19505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008338240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEX6609096.1"
                     /translation="MDLSARIRERRLALGLTLEALAARTGVSRAMLSDIERGRKNPTV
                     KLLAAIAEGLGCTVSALLGEDGGTPVAAINVVRRDERRTLVDPRSGVERHVLAPALLQ
                     RGVEVVWYEIPPGQETGAFPPHRPGVEEHITVVAGRLRCTLAGRELTLETGDSVTFRA
                     DIVHNFANAGPDPCQYLLIIL"
BASE COUNT         1203 a         2859 c         2893 g         1337 t
ORIGIN      
        1 tcagcgtcga ggtcagcctg gacctgcgcg ccgggcaggg gtcgggcacg cgcctggcgc
       61 ccatcgtcaa cctccaactg cgcgacgccg cggggcagcc cgcccccctg ggccccggcc
      121 cgatggccta cctgccgatg aacatcagcg gcaaggtcac gcgctgggtg gccgatctga
      181 gcgccccgac ggtgcccggc acctatcaca tgcacatcct ggcgcgggtg gccgggcagc
      241 ccgacatgga gttcgacctg cgcgatcccg cgttacaggt ggtggtggcg acgccgttcc
      301 agggcggcct cgtctacagc cgcaacggca acctgtggcg cagcgacgcg cagggcggcc
      361 acgcccgccg cctgacctac tacaccggcg acgggcgggc cgatagcccg gcctggtcgc
      421 ccgacggcaa gcgcatcgcc tacacgcgct cgctgcccgc cgcggccagc gacatcccca
      481 acaccgagat ctggtccatt gaccccgacg gcggctcgcc gcaggtgctg gcgccccgcc
      541 gccccggcga agacctgatc acgcccgctt tcgggccgga cggcgcgctc tacttcacct
      601 cggatcgcac cgtggacccg gcgaccaacg ccacgcccac catggaccgg ctcaccgagg
      661 gcgtggagtc gtggtcggtg gatcggctgg tgcccgccgc gcccgacggc aaacgcgcca
      721 ctgtgctgcc cgccgcccgc atgcccgacg tgagccgcga cggcaagcag atcgtctaca
      781 tcagcgcgcc cgatgccgcg gcggagagcg agtcgttcat cacccacacg ctgatgctgg
      841 ccgatagcga cgggcggaac gcgcgcccgc tgttcccgcc cgacctctac caggatgtgc
      901 tggcgccgcg cttctcgccc gacggcacgc aggtcgcctt tgccgccgtc acgccgctgg
      961 aggtgccgtc gggcgacttc gatctgctgc gggcgctggg gctggctccg ctgcccgcgc
     1021 gcgccaacgg cgtgccctgg gacatctatc tggccccggt ggcgggcggc cagatcatcc
     1081 gcctcacccg cttgaacgcc gatcagccgg tcgtggcctg ggcgaacgac ggcaaacgcc
     1141 tggcgctgct ggacgagaag ggcctcttta tgctggatct ggccgatccc gcgtcgctgc
     1201 accatgtcgg gccgccgtcg agccatgggc agttggattg gtacgaccgc taggcgcgtg
     1261 ccggcgccgc ccgcccgtca acgggcgggc tgcttcgcat gaaccaaggc cgcctgcgcg
     1321 gcctccatca ggttgccttg tccgcccgcc gtcaggtggg gtcgcctgta tcgggtgcaa
     1381 cattcgggtc aacctgcaca gtggtgatgt tgataaccgg ctgccggtag tgtactatag
     1441 aggcagtcgg gtgcgttgcc cgccggatac ctgtctggcc cggtgggcgc acggcgtctg
     1501 cggatgccgg cgcacctgcc gggaaggccc aggagccgga tcgttctcct cccgccgtgc
     1561 cacgcaccgg cccgccgctg cgctgtgggt tgccgcgaga tggaggaggc gtgaatttca
     1621 tgcgctacat gtctgtgcgt cgcgtgaccg gcgcgggact gccggcggtg ctggcgctgg
     1681 ccgccgcgct ggcctggggc ggcggcgtgg ctgtcgccca cgcccaactg agcagcagcg
     1741 atcccgcgga cggcagccag gtgactatgc cgcccccgcg gatcgtgatg cagttcagcg
     1801 aggccgtgga tccggcggcg tccacggcga cggtcagccg tatggacggt acgcgggtgg
     1861 acaacggcga tgcccaggtc gatccggcta accgccggac gctgaccgtg ggcgtgccgg
     1921 ccctgctgcc gggcggctac atcgtgcaat ggcacgccgt gacaccggac gacaacggcg
     1981 tgaccgaggg cagcttcacg ttcaccgtcg tggccgaggg tggcggcgtc gccacgcccg
     2041 ttaccgcgcc gccgtcccct gccggcggca cgggcagcat cagcggcggc ggtcccgcga
     2101 tgccacggac cgggcacgcg acggacaccg ggccgttcgc gctgccgggc gcggccctgc
     2161 tggggctggg cctggtactc ggcgggctgt ggctggtccg gcgcagccgg gccggcgcgg
     2221 caacactccg ctaaacagaa gggaggtcgg gcgcgctgcc cggccacgac aaccgcttca
     2281 tgggccattt gcctctcgcg tggtcccggt tcgggcttgc ccggctgggc gcgctcaccg
     2341 cgatcctgct gctcagcctg atctttgcgc cgcgggccgc cgcccatgcc cgcctagagc
     2401 aatccgatcc cccagccggg gccgtactgc tgcaggctcc gcccatggtc aagttgacca
     2461 tgagcgagtc gatcagcccg cagttcagcc agatccaggt gctggatcgg gcccggcggg
     2521 atgtcaccgc cgggccggtc gccgtcgatc tgcgcgaccc ggcgactatc ggcgtggtgc
     2581 tgcaacccgg cttgcccggc ggcgtctaca cggtgctctg gcgggcgatc tcggcggtgg
     2641 acgggcatct cactctcggc aatttcgcct tcacgatcca ggcgcccggc gagaccatag
     2701 cccccgcggc ccccgccccg gtgggccaga ccggagtcgc ccccgacgac ggcagcagcc
     2761 cgcccccgcc cgtgtggcgc tggatctggc gcgcgctggt gctgatcggc gcggcggggc
     2821 tgctcggcgg gccactgttc agcagcctga tcctggccgg ggcgcccgcc gcagacttgc
     2881 agccgctggt gcccgcgctg ggccggcgcc tggcgcggct ggtgctggtg tctgccgtgg
     2941 tcgtgctggg cggcttgctg gccgacatgc tgtatgagct ggcgaccgtg cgcggcaccg
     3001 atgtgcccgc cgccctcggc aacgtggccc aggtcggcga cattgtcacg acgaccggct
     3061 acggcgcttt ctgggccgtc aaggtggcgg cggcggtcgc gctgctgggc tacgcgctct
     3121 ggcgcggcgg gacgccgaac cgcccggagt ggacggcggc ggtgatgaac ggcggcttcc
     3181 tgctggccgg ggaggcgctg ggtagccatg gcgcggcggc gcggggcctc ggcgggctgc
     3241 ccctggggat gctcggcgac ctgctgcacc tgctggcggc cgccgcctgg gtgggcgggc
     3301 tgatctacct ggccgtcgtg gcgctgccgg tcgtgcgccg ggccgagccg gtggtggcgg
     3361 gacgcgcgct ggcgcgcatg gtgccgcgtt tctccaccct ggccctggtc agcatcgccg
     3421 tgctggtcgt gacgggcctg gtcaacctgg cgatccacac gctcgacccg gcggcgcttg
     3481 tggagagcga ctacgggcgc gtgctgctgg tgaagcatct gctgttcctg ccgctcgtcg
     3541 cgctggccgc cctgaaccac ggcatcgtgc gcccccgcct gatcgcgctg ctggcgcccg
     3601 gcgtccgtga ggccgcgccg cccatccgca gcctgggccg gaccatcggc gcgcaggtgg
     3661 tgctggccgt ggcggtgatg atctgcgccg ccgggctgac cttactgccg ccgcccgtga
     3721 gtgccgccgg cagcacgccc ggcagcctgc ccgcgcccgc cgcccctcca tccggcccgc
     3781 ccgcgcccac gcctaccccg gtcatcgcga gcgcgagcca gacggtgagc ggcatcggct
     3841 tcgacctgac cgccgcgccg agcggggagg gcgatcactt cacggtcgcg gtcacgcgcc
     3901 gcgacctcgc cggcccgccg ctgaccgacg tgctcaagct gcaactgcgc gtcacgccac
     3961 aggacgtgga cgcgggcagc accagccttg aggttcaccg ggtgggggct atggacccgg
     4021 atcgtcaggt ctataccgcc accgggcaga tcttcaccct gtccggcgcg tatcaactta
     4081 ccgcggaatt gctacgcacc cagggcgccg acgtgcgcgc cgggttccgc ctggtgctgg
     4141 ccgacgacgg cacgctggcg atcacgccct cgcaggtgtt gcaggcgctc gtgaccacca
     4201 accccagccc ggcgatttcg ggcacggtgc aactcaatat caagatcgtg gacggccagg
     4261 agcagccggt gcccggcgcc accctgcgcg tcaccccgtt gatgccgtcg cacggccacg
     4321 tcgaacctac gggcgatgcc gtgccggtgg cggggcagcc cggcatgtac cgcctgccgg
     4381 ctaacctgcc catgggcggg ccctggctgc tcctggtcga agtggatcgt ccgggccagc
     4441 ccccggtgaa gctggacggc acgttcaacg tgatcgatcc ccttgcgacc gctactccgg
     4501 cgtccccgta gtggtcagtg gtcagcaggt ttgtggtagt attagttgtt aagttagagt
     4561 tgggcggcca gaggtttttg tttcaggtac gattaggggc gagtgcggta gccggcggac
     4621 ggtatcagaa ggctggtcat tcgggtcaat tactgaccac tgatcactga tcactgacca
     4681 ccgaccactg accactcgcc caacggagcg aagatgaacc gacaaatgca atggcgcacc
     4741 gtgctggtgc tggtgatgat ggccggggtg ctggccgcgt gcgggggcga cgcgccgccc
     4801 gctgccgagg cgagtcgggc cacgccgcag gggcggatta tgacggcgga ccagggggcc
     4861 aacacggtca gcgtaatcga cgtggcgacc aacgaggtct atgcgacgat ccccaccggg
     4921 caatcgcccc accacgtggt gagttcgccg gacgggcgcg aaatctgggt gacgctctac
     4981 aaagagaacc atctccaggt tttcgacgcc aaaacgttta aggaactggg ccaggtggat
     5041 gtcggcggtc cgtcagacga cctgaccttt gcccccgacg gcaagcggct ctatgccagc
     5101 atgggggcgg acaacatggt ggctgtgatc gacccggcgg cgcgcaagct ggtgacgaag
     5161 gtgggcgtgg gccagacccc gcacggtgtc aaggtgcggc ccgacggcaa ggaactgtac
     5221 gtcaccaaca ccgccgagaa cacgatctcg atcatcagcc tggaaggcga tcccaagctg
     5281 gagatcagct tccgcgtcgg cgccgatccc ttcgaggtca cgtttgatcc caccggcaag
     5341 acggccttcg tcagcaactt cctgggcgac tcgattgccg tgatcgacac ggccacgcgc
     5401 aagctgaccg gcaccatccg gtcgggcaag cagccggcca tgctctcgtt tgtcgagggc
     5461 accggcggca cgctgctctg ggtcgccaac accggcacgc aggaggtgtg gatcgtggac
     5521 cccgccacgc gcaagctggt catgcgcatc ccggcgggcg gtggcgcgca tggcgtggtg
     5581 cccacaccgt cgggcaaggt cttcgtcacc aacaccaacg acaataacgt gaccgtgatc
     5641 gaggccgcga gcggcaaggt catcacgacg ctgggggtgg gtacgaaccc caacgggctg
     5701 accttcgtac cgaatacatg agattgcgcg gcatggcccg cccggcgggg ccggcgggcc
     5761 tgctcctggc gctgctggtg gcgctggtcg cgctggccgt gccccggcct gccgccgccc
     5821 acggcggtat cgtctgggtg gacgacaact acggcgcgtt ccacattgtc gtgcagacca
     5881 tcccgaccac gaaccagcac gagtggcgct tcaccgtcct ggtgaacgag ttcgacaacg
     5941 gcgaccccat cgccgacgcc cacgtgcagg tgcgcgccgc gctgacgggc acggcggatt
     6001 cgctggttga ggtgacggcg ccgccggagg ccgggcaacc cggcttctat gacgtgccca
     6061 tccgcacgcc gcgcgacggc acctgggcca tcaccgtgca cgtcgagcgc gccggcaagg
     6121 agggcaccgc cgcgtttgat atgcccgtcc agtcggccac ggactacggg gcggtcgtct
     6181 ggctgctggc ggcggtgccg attgtgctgg cggtgggcat tttcatctac tactggcgcc
     6241 ggcccctgaa agagccgccc ctcctagatc cagcggctgc ggtagaacca gaggagcatg
     6301 aggacgggga cgacgaccag ggaggccatg aggccccaga acatgagcgg gtcgtcatag
     6361 ggaatgaatt tgacgaagtt catgccgccg aagccgacga taaacgagat cggtaagaag
     6421 atcgtcgaga tgatcgtcag gcgcttcatc accgtgctca ggttgttgtt gacgctgctc
     6481 aagtacgcat ccagcacgcc cccggtcgag tcgcgctggg tatcggccag ttcgtagacg
     6541 gtaatcatca ggttgtgtac atcgcgcagg tagatctggt tctcggggcg cacgaaggcg
     6601 ttgccgggat tgctgaggac gttcagcccc tcgcgcaccg gggtggcctg ccgacggatc
     6661 gtgaccaggt tgcgcttcac ggtgaagaca tcgttgagca gatcgcggtc gggccggtcg
     6721 atgaccgtgt cctccagata gtcgatctgg tcggcggtgc gttccagcgc ttcaaaatag
     6781 gtgtcggcga tatcgtccag gatgcggtag agcaggtagt ccggcccatg attcagcggg
     6841 tgcggatctt cgtcatcctg gtgattgtcg atctcgttcc agatcttatc caccattttc
     6901 agccccgcgt cgcgcaccgt gatcaggtag ttgcggctca ggtaagcatg tagctcgtag
     6961 ctgttgatgt cgcagatcgg gtcgttggcg ggaccggggc gtggtaccgc ttgccagggc
     7021 cggcgcggcg ccggggtgcc cgcgtgcccg gcgtgctgat ccggcgcatg atgcgggccg
     7081 cccagcatgt cgctgccctc gacgaagacg gcgtgcgcga tgatgaaggc atagccttcg
     7141 tacgtctcga tcttgccccg ttcattgggg ctgcgcaggt cttcgatggt cagcgggtga
     7201 aagtggaagt agccggctag ccaatcgatg gccgccgggt cgggttcgat gatgtcgagc
     7261 cagaagagcg ggtggtcgtg cactttggtg cagccggggg gcacattctg ttcgatccag
     7321 cggcgggcgt cggcgccgtc ggtgattttg ttctccgtgg tcagtacgcg tagcggcata
     7381 gcgtctcctg catcgttcgc gcttgcagcc cggtggccgc cggagaaatc gcgagcgccc
     7441 tttgcgctca ttataacgtg gaacgccccc cggcgacacc atcacacggt gcgcgctgta
     7501 acccgccgcc tgctgcgccg cgattccgcc ttataggggc cggtttcaaa ccggctcgcg
     7561 cggtccaccc gcgttgtagg ggccggtttc aaaccggccc gccccaatcg cgttgtaggg
     7621 gccggtttca aaccggctcg cggcccattg gaatcaacgg cacgtcctag aggataatca
     7681 gcaggtactg gcaggggtcc ggcccggcgt tggcgaagtt gtggacgatg tcggcgcgga
     7741 aggtaacgga atccccggtc tccagcgtca gttcccggcc cgccagggtg cagcgcaggc
     7801 gccccgcgac cacagtgata tgctcttcga cgccgggccg gtgcggcgga aaggcgcccg
     7861 tttcctgtcc cggcgggatc tcgtaccaga cgacttccac gccgcgctgc aacagcgccg
     7921 gggccagcac gtggcgttcc accccggagc gcggatcgac cagcgtgcgc cgctcgtccc
     7981 ggcgcaccac gttgatcgcc gccacgggcg tgccaccgtc ctcgcccagc agcgccgaca
     8041 ccgtgcagcc cagtccctcg gcgatagccg ccagcagctt cacggtcggg ttcttgcgcc
     8101 cgcgctcgat gtccgacagc atcgcccggc tgacgccggt gcgcgcggcc agcgcctcca
     8161 gggtcaggcc cagggccagc cgccgctcgc ggatgcgcgc gctcaagtcc atgcccgccg
     8221 tctcctctcc gtttccgtta ttgtagcata tctctgctat agcagaaacc ggcgacttct
     8281 ccagccagga tt
//
LOCUS       DASUYC010001192         3761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1589511, whole genome shotgun sequence.
ACCESSION   DASUYC010001192 DASUYC010000000
VERSION     DASUYC010001192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1589511"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="VF276_19510"
     CDS             <1..768
                     /locus_tag="VF276_19510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6609097.1"
                     /translation="PAAAAPAVPTPVLPDINATLTAIAVSAAATQTANAAASQTAAAA
                     ATQTALALAAQQTAEAAATQTALAAAQQTAAANAALTAQAAAALTPVGLPTILKLTIE
                     SAPGTQQGARVTWVVSNAEKVTLNNDPVALTGSRPVVTSTDQALTLVATNANGSVSQS
                     TGLLLVRPPQIVSFTADQTTVAAGTEVTLSWRTLGAAKLTLNGQSVSGPDGTQKVRPT
                     ATTDYLLTAENALGPAVSLVTVNVPGTATPGAQNATP"
     gene            825..>3761
                     /locus_tag="VF276_19515"
     CDS             825..>3761
                     /locus_tag="VF276_19515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6609098.1"
                     /translation="MSIRIDLQLPQTTLELAPGDAVEVPITLTNHGGVVDAFTIEIGE
                     LPPGWYTLPANEVRLFPEQSDTLAVRFHPPAGPEAQAGGYPFSVLATSRDNPHEQTLV
                     NGIIILAATPGLLLDLQPRRVQARQALYGIMLTNPGNAPRSVVLRATDPEEALAYTLG
                     EEQPMLVAGDAPATAGVPAVVAPPLKLETPLVSGAGRLEYALDLAPGAAVMVPLQTRP
                     RKRTWFGPNQTYQFEVAAMPPGVEWEASEAQTVGGEQIYQPLLAGWGALPFALRRALL
                     IGVPLLILALILFLLLRSPGTAAPGNNLTPGADAGATQTALALAAQQTALALAAQQTA
                     AAQQTALAAAQQTALAAAAATQTALAAAGQGGATPVAGGPTAVPAGVPVNAGVPLIRA
                     FYMVVPGPGAPAGDLAANEPRLGWDVSAAQNIRLDQAALLSDLLGLPNTQLVDYRLVA
                     TGTTGVATTTLGVLLVGPPIIDSLKVSTSTVAAQDIKLVAGQVVTITQGERATLAWQV
                     QGAQKADLDGTPVPLDAQGTGRFDVTPLSSHTYIFHASNASGTVGRAVTLAVLTLTPT
                     PTPTCIVEDYVVRESAQQTMVPGTTDIGNHCDDCATSITLPFPYVLYGRPFTQAAITS
                     NGQVDFGAADATRDNVCLPDANAQDAIFAHWDDLRTDRSAGAGIYTSVSGEAPNRIFN
                     IEWRAVYADTLAPVHFEVRLYESLQQFDVIYATVGQQGRGATVGVQRDTGVSHRTEYS
                     CNLPRLNEGQMLTFYLPGCGPGGPGGPGQPDKTPFVPPTATNPPQEPGNPGGLPTATM
                     TASPTPTDTATPTVTETPQPATATVITQFTATPTGTATTTPTLPPTRERPPKERPTST
                     STAIPANTATNTPTGTPTNTPTNTPANTATLTPTPTPVCLLNDYSSTDVGQALFSYCD
                     GGCVPDTITSTLTINTPANIASIEVTNLNITSTDGGALQVYLLSPQGTTVPLFDTVCL
                     GQPWPPG"
BASE COUNT          606 a         1497 c         1179 g          479 t
ORIGIN      
        1 cccgctgccg ccgcgcccgc cgtcccgact cctgttttgc ccgacatcaa cgccacactg
       61 accgccatcg ccgtctcggc tgccgccacg cagactgcga atgccgccgc cagccagacg
      121 gccgccgctg ccgccacgca aacggccctg gcactcgccg cacagcaaac ggcagaggcc
      181 gcggccacgc agacggccct ggccgccgcg caacagaccg ccgcagcgaa cgccgcgctc
      241 actgcccagg ccgccgccgc gctcacgccc gtcggcctgc ccaccatctt gaagctgacc
      301 atcgagtctg cgccgggcac ccagcaaggc gcccgcgtca cctgggtggt cagcaacgcc
      361 gagaaggtga cgctcaacaa cgacccggtg gccctgaccg gctcccggcc cgtcgtcacg
      421 tccaccgatc aggcgctgac cctcgtcgcc accaacgcca acggcagcgt gagccagagc
      481 accggcctgt tgctggtgcg cccgccgcag atcgtcagct tcactgccga ccagacgacg
      541 gtggccgcgg gcacggaggt gacgctgtcg tggcgcacgc tgggcgcagc caaactgacg
      601 ctcaacggcc aatccgtcag cgggccggac gggacgcaga aggtgcggcc caccgcgacc
      661 accgactact tgctcaccgc cgagaacgca ctggggccgg cggtcagcct ggtcacggtc
      721 aacgtgcccg gcaccgcgac gcccggcgcg cagaacgcta caccgtaatt gccggaccgg
      781 ccccgcgcgg gccgccggct ttagttgtag gagatatcgg gaccatgtcg atccgcatcg
      841 acctccaatt gccgcagacg acgctggagc ttgcgcccgg cgacgccgtg gaggtgccta
      901 tcaccctgac caaccacggc ggcgtggtcg atgcgttcac catcgagatc ggcgagttgc
      961 cgccgggctg gtacaccctg cccgctaacg aggtccgact cttccccgag cagagcgaca
     1021 cgctggcggt ccgcttccac ccgcccgccg ggccggaggc ccaggccggc ggctatccct
     1081 tcagcgtcct cgccacgtcg cgcgacaacc cgcacgagca aaccctggtc aacggcatca
     1141 tcatcctggc ggccacgccc ggcctgctgc tcgacttgca gccgcgtcgc gtgcaggccc
     1201 gccaggcgct ctacggcatc atgctcacca accccggcaa cgcgccccgg tccgtcgtgc
     1261 tgcgggcgac cgacccggag gaagccctcg cctacaccct gggcgaggaa cagccgatgc
     1321 tggtggccgg cgatgccccg gcgacagccg gcgtacccgc cgtggtcgcc ccgccgctga
     1381 agctggagac gcccctggtg agcggggcgg gccgcctgga gtacgcgctg gacctggcgc
     1441 cgggcgccgc ggtcatggtg ccgctgcaaa cccgcccgcg caaacgcacg tggttcggcc
     1501 ccaaccagac ctaccagttc gaggtggctg ccatgccgcc cggcgtcgaa tgggaagcct
     1561 ccgaggccca gacggtcggc ggtgagcaga tctaccagcc gctgctggcc gggtgggggg
     1621 cgctgccttt cgccctgcgc cgcgcgctgc tcatcggcgt gccgctgctc atcctggcgc
     1681 tgatcctgtt cctgctgctg cgctcgccgg gcaccgccgc gccgggcaac aacctgacac
     1741 ccggcgccga tgcgggcgcc acgcagaccg cgctggccct ggccgcgcaa cagacggcgc
     1801 tggccctggc cgcccagcaa accgctgccg cgcagcagac cgccctcgcc gccgcgcaac
     1861 agacggcgct ggccgcggca gccgccacgc agacggctct ggccgccgcg ggccagggcg
     1921 gggccacacc ggtcgccggc gggcccaccg ccgtgccggc gggcgtgccg gtgaacgcgg
     1981 gcgtgccgct gattcgcgcc ttctacatgg tggtgcccgg ccccggcgcc ccggcgggcg
     2041 atctcgcggc taatgagccg cgcctgggct gggatgtgag cgccgcgcag aatatccgcc
     2101 tggaccaggc cgcgctgctg tccgatctgc tggggttgcc caatacccaa ctcgtcgatt
     2161 accgcctggt cgccaccggg accaccggcg tggcgaccac cacgctcggc gtgctgctgg
     2221 tcgggccgcc catcatcgac tcgctcaagg tcagcacgtc caccgtggcc gcgcaggaca
     2281 tcaaactggt cgccgggcag gtcgtcacca tcacccaggg cgagcgagcc acgctggcct
     2341 ggcaggtcca gggcgcccag aaggccgacc tggacggcac acccgtgccc ctcgatgccc
     2401 agggcacggg ccgcttcgac gtgaccccgc tcagtagcca cacctacatc ttccacgcct
     2461 ccaacgcctc gggcaccgtg ggccgggccg tgaccctggc cgtgctgact ctcacgccca
     2521 cccccacgcc cacgtgtatc gtcgaggact acgtggtgcg cgagtccgcc cagcagacga
     2581 tggtgccggg cacgaccgac atcggcaacc attgtgacga ctgcgccacc tcgatcacgc
     2641 tgccgttccc ctatgtgctc tatggccggc ccttcacgca ggctgcgatc acatccaacg
     2701 gccaggtcga cttcggcgcc gccgatgcca cccgcgacaa cgtgtgcctg cccgacgcca
     2761 acgcccagga cgccatcttc gcccactggg acgacctgcg caccgaccgg tccgccggcg
     2821 cgggcatcta cacgtcggtc agcggcgaag cgcccaaccg catcttcaac atcgagtggc
     2881 gcgccgtcta cgccgatacc ctcgccccgg tccacttcga ggtccggctg tacgaatcgc
     2941 tgcaacagtt cgacgtgatc tatgccacgg tgggccagca gggccgcggc gccacggtgg
     3001 gcgtgcagcg cgataccggc gtcagccatc gcaccgaata ctcctgtaac ctgccgcgcc
     3061 tcaacgaagg ccagatgctg accttctacc tgcccggctg cggtcccggc ggccccggcg
     3121 gtcccggcca acctgataag acgccgttcg tgccgcccac ggccaccaat ccgccgcagg
     3181 aaccgggcaa ccccggcggg ctgcccacgg cgactatgac cgccagcccg acgccgaccg
     3241 atacggccac gccgaccgtc accgaaacgc cgcaacccgc gacggcgacc gtgatcaccc
     3301 agttcacggc cacgcccacg ggcacggcca ccaccacgcc gaccctaccg ccgactcgtg
     3361 agcgcccgcc gaaagagcgg cccacgtcca caagcaccgc gataccggcg aacacggcga
     3421 cgaacacacc gaccggtacg ccgacgaaca cgccgacgaa cacaccggcg aatacggcca
     3481 ccttgacccc gacgccgacc ccggtgtgcc tgctcaacga ctactccagc acggatgtcg
     3541 gtcaggcgct gttctcctac tgtgacggcg ggtgcgtgcc ggatacgatc acgtccacgc
     3601 tgaccatcaa tacgccggcc aatatcgcca gtatcgaggt aacgaacctc aatatcacgt
     3661 ccacggacgg cggggcgttg caggtctatc tgctcagccc gcaggggacg accgtgccgc
     3721 tgttcgacac cgtctgcctg ggccagccct ggccgccggg c
//
LOCUS       DASUYC010001193         4914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1609183, whole genome shotgun sequence.
ACCESSION   DASUYC010001193 DASUYC010000000
VERSION     DASUYC010001193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4914
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1609183"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1585)
                     /locus_tag="VF276_19520"
     CDS             complement(<1..1585)
                     /locus_tag="VF276_19520"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6609099.1"
                     /translation="MTVHGRSYRAVLLIMLAGLLLPLLAACGGETPTATSVPAAPPTN
                     TTAPAAATDTPAMQAQPTNTTAAAPEATATTSSASAGAVDLNQPAACANVTAGLQGKK
                     LHILSNVVGGKTPEENDLFVKELERLTGADIDFVKPASDYDQKLLADVSAGTAYDLIY
                     MTKPTMDILVDQKALTPLTDKIKASPVLGDPKVIPPAEWDIIRYGDGQIYSVYQKFEG
                     GTMPIVRQDWMDKLHLQEPKTLDDFYNVLKAFKEQDPDGNGKADTYGLSTSGLYDIQG
                     FMSAAGVKSHYVIDANGKRSIPFATDAAIPVYEWLAKLYKEGILDPNFATNSTSQMRE
                     LFLADRVGMITYWDAWVGLLNNTRMTKDPNTTFMAKGIAGATGPDGKIMLRRGDPSVF
                     AIPVNAPDPDAAFKFLEFWNCPAGILLGSLGIEGNDYTVQDGKYTLTKTGQEHGMDHG
                     VPRWYNSYFARPLPLPPGVTDAQKIILDNNATIEIPTAQWTDANKIINDYAFKAINGQ
                     MPVADAVHQMHDELKAKNLI"
     gene            2081..2806
                     /locus_tag="VF276_19525"
     CDS             2081..2806
                     /locus_tag="VF276_19525"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX6609100.1"
                     /translation="MAHYRLLALDYDGTLATRGVIAEPTERALCAAKQAGIRLVLATG
                     REVEPLIEACPQIGLFDLVVAENGGLLYFPARDAIEHLAEPRPVELLEELQRRGVFFR
                     RGRVILSTRVEEMPVIQDAIARLKLDLPVTYNKDSAMFLPPGIDKASGLRAGLRRLGV
                     DPSACVGMGDAENDICFVEVCGLVVAPANAIAELKAVAGLVMTHENGAGVAEFIYEHL
                     LAGVGPGSSRDAAAPEMGAAPGA"
     gene            complement(2803..>4914)
                     /locus_tag="VF276_19530"
     CDS             complement(2803..>4914)
                     /locus_tag="VF276_19530"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX6609101.1"
                     /translation="DVGGTFTKAAAIYSRPLRIVHQVVVPTTHGAPEGVARGVVEALE
                     RLLASPTLCRFQPRVVAHSTTQAVNALLEGDTAPVGIIGMAREEDRREAEKRTRVGDI
                     TLAPGRTLRTLHRFLDTTDGLSRHDVVQAINELVAQGARAIVASEAFSVDDSANERLV
                     MSAAREQGLPATGGHEVSGAYGLEVRTLTAAINASIMPRMMQTAEMVEGCLGQAHVHS
                     PLVVMRGDGGVMSMQQLRERPLLTVLSGPAASLAGALLYGRVLDGIFLEVGGTSTNLG
                     VIRGGQPAMKYVQVMDHPTCLRSLDVRVQGVAGGSMARVRGKKLADVGPRSAHIAGLA
                     YSCFARPEELDGPLTIELFAPRAGDPADYVAVQNAAGKRFALTVTCAANALGRVAEGA
                     YAYAPAEAALKAFAPLGRLLGCSAEGAAERLLDRAAEQVAAAVRALCVEYRLDRKDLD
                     LVGGGGGAAALGPAVAAKIGARWALADHAEVISSIGVAMAMVREEAEKTLENPGPEDV
                     AQLAALVESRAISSGAEPASVQVFTEALPEKGGIRAVAIGNVGLDAGGEREIDAAEAL
                     TIAARRVQAPPGKLLAVGQTGHYFVFAPPARKGWPWAKRYPVIVVDRHGGVRLEAEDA
                     EVLTGTPAEVLAALRRALTGEQGVGAFGLGPRLYLLTGPHLLDLSSMQQSADSLAAAE
                     RALAGATSDAVVAVLQRPRLL"
BASE COUNT          714 a         1652 c         1711 g          837 t
ORIGIN      
        1 cgatcaggtt tttcgccttc agttcgtcgt gcatctggtg cacggcatcg gccaccggca
       61 tctggccgtt gatcgccttg aaagcgtagt cgttgatgat cttattggcg tccgtccact
      121 gcgcggtcgg gatctcgatg gtggcgttgt tgtccaggat gatcttctgg gcatcggtca
      181 cgccgggggg cagcggcaac gggcgcgcga agtagctgtt gtaccagcgc ggcacgccat
      241 ggtccatgcc gtgttcctgg ccggtcttgg tgagggtgta cttgccgtcc tggacggtat
      301 agtcgttgcc ctcaatgccc agcgaaccga ggaggatccc ggcggggcag ttccagaact
      361 ccaggaactt aaaggcggca tccgggtcgg gcgcgttcac cgggatggcg aagacgctgg
      421 ggtcgccgcg gcgcagcatg atcttgccgt cggggccggt cgccccggcg atgcctttcg
      481 ccataaaggt cgtgttcggg tccttggtca tgcgcgtgtt gttgagcagg cccacccagg
      541 cgtcccagta ggtgatcata cccacgcggt cggccaggaa gagctcccgc atctggctgg
      601 tgctgttggt ggcgaagttg ggatcgagga tgccctcttt gtacagcttc gccagccact
      661 cgtacaccgg gatggcggca tcagtcgcga acgggatgga gcgcttgccg ttggcgtcga
      721 tcacatagtg cgatttcacg ccggcggcgc tcatgaagcc ctggatgtcg tacaggcccg
      781 aggtcgagag gccataggtg tcggccttgc cgttgccgtc cgggtcctgc tctttgaacg
      841 ccttgagcac gttatagaag tcgtccaggg tcttcggctc ttgcagatgc agtttgtcca
      901 tccagtcctg gcgcacaatg ggcatggtgc cgccctcgaa cttctgatag acggagtaga
      961 tctgcccgtc gccgtagcgg atgatgtccc actccgcggg cgggatcacc ttggggtcgc
     1021 ccaggaccgg cgaggccttg atcttgtcgg tcagcggcgt cagcgccttc tgatcgacca
     1081 ggatgtccat cgtcggcttg gtcatgtaga tgaggtcata cgccgtcccc gccgacacgt
     1141 cggccagcag tttctggtca tagtccgacg ccggcttgac gaagtcgata tcggcgcccg
     1201 tcagccgctc cagctccttg acgaagaggt cgttttcttc gggcgtcttg ccgccgacca
     1261 cgttgctgag aatgtgcagc ttcttgccct gcaggcccgc ggtgacattg gcgcaggcgg
     1321 cgggctggtt caggtccacg gcgccggcac tagcgctgct ggtggtggcg gtggcctccg
     1381 gcgcggcagc ggtggtgttg gtcggttggg cctgcatggc cggggtgtcg gtcgcggcgg
     1441 cgggggccgt cgtgttcgtg ggcggcgccg ccggcacgct cgtcgcagtg ggggtttcgc
     1501 ccccgcaggc tgccagcagc ggcagcaaca acccggcgag catgatcaac aggacggcgc
     1561 ggtaactcct tccatggacg gtcataaata cttgcctcct ttggcgttca gtcgtggccc
     1621 ggcgccctcc ctgccagggt tacggccccg gcccgactct cctgtgtagc cccggttcta
     1681 ttcagtcgcc agggcggctg ctacaataat cgggaatccg atcgcccgtt ttgtagataa
     1741 agattcacca cctcctttca cagtcgtggt agaaagcgtg cctccctatc gcggggatgc
     1801 tgccggcgga aacgagcgcg tcaggcgcag cagaacggtc agcagcattc ctcacccagc
     1861 atagtggccg gtgaggtccg aacggcgact cggcccgggg tacagcaaat cctgagcgat
     1921 catccggcgt accgtgttat gggcacgcgc ttcccgtttc gttaaatgcc ttaccgccgt
     1981 gcattttaca ctaatcgaga ccgctcggca atacaatcgc cgagaatctg tatctgaaca
     2041 cgacctatcg tataatgcaa attacgacat aggagatgag atggcacact accggctgct
     2101 ggccctggac tacgacggca ccctcgccac ccgcggcgtg atcgccgagc cgaccgagcg
     2161 ggcgttgtgc gcggccaagc aagccggcat ccggctggtg ctcgccacgg ggcgcgaggt
     2221 cgagccgttg atcgaggcgt gcccgcagat cgggctgttc gacctggtgg tggccgaaaa
     2281 cggcgggctg ctctacttcc ccgcccgcga cgcgatcgag cacctggccg aaccgcgccc
     2341 cgtggaactg ctggaggaac tacagcgccg gggcgtgttc ttccggcgcg ggcgggtgat
     2401 tctcagtacc cgcgtggagg agatgccggt cattcaagat gcgattgccc ggctcaagct
     2461 cgacctgccg gtgacgtaca acaaggactc ggccatgttt ctgccgccgg ggatcgacaa
     2521 ggcgagcggc ttgcgggcgg ggctgcgccg gctcggcgtc gacccgagcg cgtgcgtggg
     2581 catgggcgac gccgagaacg acatctgctt cgtggaggtc tgcgggctgg tggtggcgcc
     2641 ggccaacgcc attgcggagc tgaaagccgt ggccgggctg gtgatgacgc acgagaacgg
     2701 ggcgggcgtg gccgagttta tctacgagca cctgctggcc ggcgtgggac ccggtagcag
     2761 ccgggatgcg gccgcgccgg agatgggcgc ggcgcccggc gcttagagca gccggggtcg
     2821 ctgcaacacc gccacgacgg cgtcgctggt cgccccggcc agggcgcgct cggcggcggc
     2881 caggctgtcg gcgctctgct gcatgctcga caggtcgagc agatgcggcc cggtcagcag
     2941 gtagagccgc ggtcccaggc cgaaggcgcc cacaccttgc tcgccggtca gcgcccggcg
     3001 cagcgcggcc agcacctccg cgggggtacc ggtcagcacc tcggcgtcct cagcttcgag
     3061 gcgcaccccg ccgtgacggt ccacgacgat caccgggtag cgtttggccc agggccagcc
     3121 cttgcgcgcg ggcggcgcaa acacgaaata gtgcccggtc tggcccacgg ccagcagctt
     3181 gcccggcggc gcctgcacac ggcgcgccgc gatcgtcagc gcctccgcgg cgtcgatctc
     3241 gcgctcgccc ccggcgtcca ggcccacgtt gccgattgcc acggcgcgga tgccgccctt
     3301 ttcgggcagc gcctcggtga acacctgcac gctggccggc tcggcgccgc tgctgatggc
     3361 ccgcgactcc accagcgcgg ccagttgggc tacgtcctcg ggaccggggt tctccagcgt
     3421 cttctccgcc tcttcgcgca ccatggccat ggcgacgccg atggatgaga tgacctcagc
     3481 gtggtcggcc agcgcccagc gcgcgccgat cttggcggcg accgccggac ccagcgccgc
     3541 cgcgccgccg cccccgccga ccaggtccag gtccttgcgg tcgaggcgat actccacgca
     3601 aagcgcgcgc accgccgcag ccacctgctc ggcggcgcgg tcgagcaggc gctcggcggc
     3661 cccctcagcc gaacagccca gcagccggcc cagcggcgcg aacgccttca aggcggcctc
     3721 cgccggcgca taggcatagg cgccctcggc cacgcggccc agggcattgg cggcgcaggt
     3781 gacggtcagg gcgaagcgct tgccggcggc gttctgcacg gccacatagt ctgcggggtc
     3841 gccggcgcgc ggcgcgaaca gttcgatcgt cagcgggccg tccagctctt cgggccgggc
     3901 gaagcaggag taagccagcc cggcgatgtg cgcgctgcgc gggccgacat cggccagctt
     3961 cttgccacgc acccgcgcca tgctgccgcc cgccacgccc tgcacgcgca cgtcgagcga
     4021 gcgcaggcag gtggggtggt ccatcacctg gacatacttc atggccggct ggccgccgcg
     4081 gatgacgccc aggttggtgc tggtgccgcc cacttcgagg aagatgccgt cgagcacgcg
     4141 cccgtagagc agcgccccgg ccagcgacgc cgccgggccg gagagcaccg tcagcagcgg
     4201 gcgctcgcgc agttgctgca tgctcatcac gccgccgtcg ccgcgcatca ccaccagcgg
     4261 cgagtggaca tgcgcctggc ccaggcagcc ttcgaccatc tcggcggtct gcatcatgcg
     4321 cggcataatg ctggcgttga tcgccgcggt cagcgtccgc acttcgaggc cgtaggcgcc
     4381 ggagacttcg tggccgccgg tggcgggcag gccctgttcg cgggccgcgc tcatcaccag
     4441 ccgctcgttg gcgctgtcgt ccacgctgaa cgcttcgctg gcgacgatgg cccgcgcgcc
     4501 ctgggccacc agttcgttga tcgcctggac cacgtcgtgc cgcgacagcc cgtcggtcgt
     4561 gtcgaggaag cggtggaggg tacgcagcgt gcggccaggc gccagggtga tgtcgcccac
     4621 gcgggtgcgc ttctcggcct cgcggcggtc ttcctcacgc gccatgccga tgatgcccac
     4681 gggggccgta tcgccttcga gcagcgcgtt gaccgcctgg gtggtgctgt gggccacgac
     4741 gcgcggctgg aagcggcaga gtgtaggcga cgccagcagg cgctccagcg cctcgaccac
     4801 gccccgcgcc accccttcgg gcgcgccgtg cgtggtcggc acgaccacct ggtggacgat
     4861 gcgcagcggg cggctgtaga tcgccgccgc cttggtgaag gtgccgccga catc
//
LOCUS       DASUYC010001194         4634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1135111, whole genome shotgun sequence.
ACCESSION   DASUYC010001194 DASUYC010000000
VERSION     DASUYC010001194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4634
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135111"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VF276_19535"
     CDS             <1..447
                     /locus_tag="VF276_19535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6609102.1"
                     /translation="DFGQDQGCEAPVYRGCTRAAAGTAGAGPAAGAIAAEAATGPAAG
                     AIAAEAAARAIAATGAIAAEAATGAITAARGAIAAEAATGATGAGGPAGRRASGARST
                     PAGGGHIAGFFRVVGPVAVGNWPAWSGRASRGSRVDFSILWHSCTS"
     gene            complement(465..851)
                     /locus_tag="VF276_19540"
     CDS             complement(465..851)
                     /locus_tag="VF276_19540"
                     /inference="COORDINATES: protein motif:HMM:NF016204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEX6609103.1"
                     /translation="MKAKHPAPRPKHVPQRTCVACRTVESKRGMVRIVRTPTGRVEID
                     PTGKRSGRGAYLCRNRACWTAALQKKSLEYALKTAINLEDKAMLQAYSETLPASATDA
                     GGDAAPVASEESGSPPGPSEGLTKPT"
     gene            complement(954..2402)
                     /gene="nusA"
                     /locus_tag="VF276_19545"
     CDS             complement(954..2402)
                     /gene="nusA"
                     /locus_tag="VF276_19545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX6609104.1"
                     /translation="MKGEFYAAIAQIASERSLAKDVILESVEAALISAYKRMTGSDQS
                     VTVRIDPASGQAHVFAQKTVVEQVEDPRAEISLADARALEPGAQLGDTVQVETTPANF
                     GRIAAQTAKQVVMQRIREAERDRFFSEYIDRVGDIVNVMVQRVDPRSVDPARPEQPGA
                     IIVEIGKAEAVLPPNEQVKTERYRVGQRMRVLLLDVQRNQRGPQLIVSRAHKNMLRRL
                     LELEVPEIHNGTVEIKAIARESGMRSKVAVAARQEGLDPVGACVGMRGIRIQNIVNEL
                     YGEKIDIVSWHPDMAVFIANALSPAQVVSVELLEQEKTAQVIVPERQLSLAIGKEGQN
                     ARLAAKLTGWRIDIKSATASMHEPEEDFARRAVEALRAEAGNGFDSLAAFPAAAPEAM
                     RDDRRKVRANNTVVYQNRVFGPLPENLVGETVRLRLTDAGLQVQTPTRIVATYPWEQA
                     QALDATEARPEDDMEPADMAPPDSPATEITES"
     gene            2704..3192
                     /locus_tag="VF276_19550"
     CDS             2704..3192
                     /locus_tag="VF276_19550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6609105.1"
                     /translation="MQQEPGDSGVAPPPYAYYIIDEVLNYGGFFGGNTVTIYAHTPAT
                     PDDDRKFIIAEQNFENLRDGDRFKVTEGQMLGLVLDGDEVRSARLVGAPTREGLRAAL
                     APPRPLPGHELEVRSLAYHCGHCNLWIAGQPPRQGGDGPYLCAICGNPLQPTQPDSER
                     RP"
     gene            3201..4283
                     /locus_tag="VF276_19555"
     CDS             3201..4283
                     /locus_tag="VF276_19555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007502809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEX6609106.1"
                     /translation="MDDTLKMLEELTQALGVPGQEDEVRGVLSRYLAPYGELVRDNLG
                     GVAVRKVGTDAAAENGPRVLIAGHMDEVGYMVSLVTAEGYIRFQPLGGWWEQVMLAQR
                     VLIRTRKGDIMGVTGSKAPHILPPEERNKIVQKRDMYIDIGARSKDEVLAAGVRPGDP
                     VVPICPFTVMSNPDMLLAKAWDNRIGCAIAVETCRLLNDEAHPNVLYAGATVQEEVGL
                     RGAATLTHLTAPDVAIALDVGIAGDTPGVKSEEAQGKLGAGPVVLLYDSSLIPNTRLR
                     DLVSDVAEAEGIPLQYDLMAAGGTDAGRMQLFGPGVPSIAIGVPARYIHSAASMISRS
                     DFEQAAQLVAALVRRLDAETVAGLRG"
BASE COUNT          708 a         1558 c         1557 g          811 t
ORIGIN      
        1 gactttgggc aggatcaggg gtgtgaggcg ccggtctacc gtggatgcac gagggccgcc
       61 gccgggaccg ccggggccgg gccggccgcc ggggcgattg ccgccgaagc cgccaccggg
      121 ccggccgccg gggcgattgc cgccgaagcc gccgccaggg cgattgccgc caccggggcg
      181 attgccgccg aagccgccac cggggcgatt accgccgcca ggggggcgat tgccgccgaa
      241 gccgccaccg gggccaccgg ggccggcggg ccggcgggcc ggcgggccag cggggcgagg
      301 agcacgcccg cggggggcgg gcacattgcg ggtttcttcc gcgtcgtcgg ccccgtcgcc
      361 gttggtaact ggcccgcgtg gagcgggcgt gcgagccggg gtagcagggt cgatttttct
      421 atcttgtggc attcttgtac ctcctgagcg accctgctgt gaccttatgt gggcttagtc
      481 agcccctccg aaggtccggg gggagacccg gactcttcgc ttgccacagg ggccgcgtca
      541 ccgcccgcgt ctgttgcgga tgcaggcagg gtttcactat atgcctggag catagcctta
      601 tcctccaggt tgatcgccgt tttgagtgca tactctaacg actttttttg cagtgctgcg
      661 gtccagcacg cacgattgcg gcagagatag gcgccgcgcc cggagcgctt cccggtcgga
      721 tcgatttcca cccgcccggt gggcgtgcgc acgatccgga ccattccacg ctttgactca
      781 acggtccggc aggccacgca ggtgcgctgg ggcacatgct tgggccgcgg ggccggatgc
      841 ttcgctttca tgctaacttt ccgctttcag ccgccagggg ccggtgggcc ggcccgctga
      901 ccactgtgcg cgcaacggtg ctgcgtacac cgcgcggtgg cgcccgccca cccctagctt
      961 tccgtgattt ccgtcgccgg agaatcgggc ggcgccatgt ccgccggttc catatcgtct
     1021 tcgggtcgcg cttcggtggc gtccagcgcc tgcgcttgct cccacggata cgtggcgaca
     1081 atgcgtgtcg gcgtctggac ttgcagcccg gcgtcggtca gccgcaggcg caccgtttcg
     1141 ccgaccaggt tctcaggcag cgggccgaac acgcggttct gatagaccac cgtgttgttg
     1201 gcccgcacct tgcgccggtc gtcgcgcatg gcttcgggcg cggcggcggg gaaggcggcc
     1261 aggctgtcga agccgttgcc cgcttccgcc cgcagggcct ccacggcgcg ccgggcgaag
     1321 tcttcctccg gctcgtgcat agacgccgtc gcgctcttga tgtcgatgcg ccagccggtc
     1381 agcttggccg ccaggcgggc gttctgcccc tctttgccga tggccagcga caactgccgc
     1441 tcgggcacga tcacctgcgc ggttttctcc tgctccagca actccacgct gaccacctgg
     1501 gccgggctga gcgcgttggc gatgaacacc gccatgtcgg ggtgccacga cacgatgtcg
     1561 atcttttccc cgtacaattc gttaacgata ttctggatgc gtatgccgcg catgccgacg
     1621 caggcgccca cggggtcgag cccttcttgc cgggccgcga cggcgacctt cgagcgcatc
     1681 ccggactcgc gggcaatcgc cttgatctcg accgtgccgt tatggatctc cggcacttcg
     1741 agttccagca gccggcgcag catattcttg tgcgcgcgcg acacgatcaa ctggggcccg
     1801 cgctggttgc gctgcacgtc gagcagcaac acgcgcatcc gctggcccac ccggtaacgc
     1861 tcggtcttga cctgctcgtt gggcggcagc accgcttcgg ccttgccgat ctcgacgata
     1921 atcgcgccgg gctgttcggg ccgggccggg tccaccgacc gcgggtcgac ccgctggacc
     1981 atgacgttga cgatatcgcc gacgcggtcg atatattcac taaagaaacg gtcgcgctcc
     2041 gcctcgcgga tgcgctgcat gacgacctgc ttggccgtct gtgccgcgat gcgcccgaag
     2101 ttggccgggg tggtctccac ctgcacggta tcgcccagtt gcgcgccggg ttccagggca
     2161 cgcgcatccg ccaggctgat ctccgcccgc gggtcttcta cctgttccac gaccgttttc
     2221 tgggcgaaga cgtgggcctg cccgctcgcc gggtcaatcc gcacggtaac gctctggtcg
     2281 ctgccggtca tgcgcttgta cgccgaaatc agcgctgctt ccacactctc caggatgaca
     2341 tccttggcga gggaccgctc gctggcgatt tgcgcaatgg cagcgtagaa ctctcccttc
     2401 atcgcattcg ccctcctgct cttaatctgt actccctgtt gcgccgcctc ggggtttccc
     2461 aaaaataccc ctaacaacac gaaaagtggg ggtgacccac ttttcggagg cggtgttggt
     2521 gctgccgtaa ttatatcatt ttggcggcgt ttccgcaagg cacaccgcgg cgcgccggta
     2581 aggtgccgcc gggtagcccg tccactagag tacggaatcg cgcccgcccc ggatgcgcgg
     2641 gcgattgttc cccggcgggg gcgcgtggta taatgcggca tctggcgcga tggaggaaac
     2701 tcgatgcaac aggaaccggg cgacagcggc gtcgctcccc cgccttacgc gtattacatc
     2761 atcgacgagg tgctcaacta cggcggcttt ttcggcggca ataccgtcac gatctacgcc
     2821 cataccccgg cgacccccga cgacgaccgc aagttcatca tcgccgagca gaacttcgag
     2881 aacctgcgtg acggcgaccg cttcaaagtg accgagggcc agatgctggg cctggtgctg
     2941 gacggcgacg aggtgcgctc cgcccgcctg gtgggcgcgc ccacccgcga aggcttgcgc
     3001 gccgcgctcg cgccgccccg ccccctgccc ggccacgagc tcgaagtccg ctccctcgcc
     3061 taccattgcg gccactgcaa cctctggatt gccggccaac cgccccgcca gggtggggac
     3121 ggcccgtacc tctgcgcgat ctgcggcaat cctttgcaac ccacccaacc cgactctgag
     3181 aggagaccgt agagatcccg atggacgata cgctgaagat gctggaggag ttgacccagg
     3241 cgctgggcgt gccggggcag gaagacgagg tccgcggcgt gctcagccgc tacctggcgc
     3301 cttatggcga actggtgcgc gataacctgg gcggcgtcgc cgtgcgcaag gtcggcaccg
     3361 acgccgccgc cgagaacggc ccgcgcgtgc tgatcgccgg gcacatggac gaagtcggct
     3421 acatggtctc gctggtgacg gcggagggct acatccgctt ccagccgctg ggcggctggt
     3481 gggaacaggt gatgctggcc cagcgcgtcc tgatccgcac ccgcaagggc gacatcatgg
     3541 gcgtgaccgg ctccaaggcg ccgcacatct tgccgccgga agagcgcaac aagatcgtgc
     3601 agaagcggga catgtatatc gacatcggcg cgcgcagcaa ggacgaagtg ctggcggcgg
     3661 gcgtgcgccc cggcgatccc gtcgtgccga tctgcccctt cacggtgatg agcaaccccg
     3721 acatgttgct ggcgaaagcc tgggacaacc gcatcggctg cgccattgcc gtcgagacct
     3781 gccgcctgct gaacgacgag gcgcacccca acgtgctcta cgccggagcc acggtgcagg
     3841 aggaagtggg cctgcgcggc gccgccacgc tgacccacct gaccgcgccc gatgtcgcca
     3901 tcgcgctcga cgtgggcatc gccggggata cgccgggcgt caagtccgag gaagcgcagg
     3961 gcaaactggg ggcggggccg gtcgtgctcc tctacgacag cagcctgatc cccaacacgc
     4021 gcctgcgcga cctggtgagc gacgtggccg aggccgaggg catcccactg caatacgacc
     4081 tgatggccgc gggcggcacc gacgccgggc ggatgcagct tttcgggccg ggcgtgccct
     4141 cgatcgccat cggcgtgccc gcccgctaca tccacagcgc cgcctcgatg atcagccgca
     4201 gcgacttcga gcaggcggcg caactggtgg ccgccctggt gcgccgcctg gatgcggaaa
     4261 cggtggccgg gctgcgcggc taagcgcgac gatttatccg ttcaccgcga aggaacgaag
     4321 aacgcgaaga ttattgacat tagttttgtt cttcgcgctc ttcgcgtctt cgcggtgtag
     4381 aataagcata catgaacggg tgtcgggcaa aaagaagggc ggctgcgagg cacgcagcca
     4441 cccttttgcg tggccggagg gcttattgca gcgggatgat aatgttctgc ccggcaaaga
     4501 tcgcattcgg gttgctcatg ccgttgcgct gggtgatggc gctgatcgag acgttgaaca
     4561 gttgcgagat atgggtcagg ttatcgccgg agcgaatggt gtactgcagc tcggtcggct
     4621 tgtagtcggc gggc
//
LOCUS       DASUYC010001195         2276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1609437, whole genome shotgun sequence.
ACCESSION   DASUYC010001195 DASUYC010000000
VERSION     DASUYC010001195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1609437"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="VF276_19560"
     CDS             <1..776
                     /locus_tag="VF276_19560"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEX6609107.1"
                     /translation="LLWDDTNLYVAAHVFSPEHHQTETGPSVWKGDTLWIYLDSNHDK
                     QSVETKLTLAQTPSGPQVWEWQSGFFLPDAKLTWQQVPGGYIYEAALPWKSLNVRAPK
                     AGQQMGINLGRGCCGTGFMDLSGTDPDIAKNEVQLTLVDTLSAGSKDQVQHPSGPDAV
                     GMRWQLDDRGPGRQLHGGASDRDYLTLERLASAPFDLKAGAHSLRLEYAGTDPTRQIS
                     VDGVLLMPTVLHKTLRDAGGRQIDVTYQIDGGKWSATGP"
     gene            779..1849
                     /locus_tag="VF276_19565"
     CDS             779..1849
                     /locus_tag="VF276_19565"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX6609108.1"
                     /translation="MPMTIKDIAARLGVSTATVSRALNDKPGVSDEVRRRVLDLAADL
                     SFAPNGTGRGLSTARTQTLGFVVRRKAFPTVQDHFYLQIMHGVEEEVARNGYHVILSA
                     VGAPAGANTMNGANDLSLVREGRVDGLILAGPDIDSSLILELQHNGVPLVLVDNALRQ
                     TPIDCVLNDDREGAFAAVSHLIEHGYERIAFIGGPSTWISTVERRAGFEDALAAHNKT
                     ACAIVHQRETTVETGYAAMIELLEQDTRPNAVFAVNDSMAFGAMRAARENGLTVPGDL
                     ALAGFDDIDAAQHTTPPLTTVRIFKERVGEVAARRLLDRLTGPATPPQRTYVGTELII
                     RQSCGCPPAPIRREVVSAADTP"
     gene            1928..>2276
                     /locus_tag="VF276_19570"
     CDS             1928..>2276
                     /locus_tag="VF276_19570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6609109.1"
                     /translation="MERIHRMFALLATLALLLPLLAACGGETPATTAPAAATNTTAPA
                     PAAAATDTPASAAAAPTNTTAPAAGNAGGEKVTLTMATWAGADESKELQAIIDKVNAA
                     ATDYQIVYNPAPAD"
BASE COUNT          411 a          827 c          725 g          313 t
ORIGIN      
        1 acttgctgtg ggacgacacc aacctctacg tcgccgcgca cgtcttttcg cccgagcacc
       61 accagaccga gaccggtccc tctgtctgga agggcgatac gctctggatc tacctggaca
      121 gcaaccacga caagcagagc gtggagacca aattgacgct ggcccagacg cccagcgggc
      181 cgcaggtctg ggagtggcag agcggcttct tcctgccgga cgccaaactc acctggcagc
      241 aggtgcccgg cggctacatc tacgaggcgg ccctgccctg gaagtcgctc aacgtgcgcg
      301 cgcccaaagc gggccagcag atgggcatca acctgggccg gggctgctgc ggcaccgggt
      361 tcatggacct gtcgggcacc gatcccgaca tcgccaaaaa cgaggtccaa ctgacgctgg
      421 tggacacgct gtcggcgggc agcaaagatc aggtgcagca tcccagcggc cccgatgcgg
      481 tgggcatgcg ctggcaactc gacgaccggg ggccgggccg ccagttgcac ggcggcgcct
      541 cggaccgcga ttacctgacc ctggagcggc tggcctcggc gccgttcgac ctgaaggcgg
      601 gcgcccacag cctgcgcctg gaatacgcgg gcaccgatcc cacgcgccag atctcggttg
      661 acggcgtgct gctcatgccc acagtgctgc ataaaacctt gcgcgatgcc ggcggccggc
      721 aaatcgacgt aacctatcag atcgacggcg gtaagtggag tgcaacagga ccataaccat
      781 gccgatgacg attaaggaca tcgcggcgcg gctgggcgtt tcgacggcca ccgtctcccg
      841 cgcgctcaac gataagccgg gggtgagcga cgaggtgcgg cgccgggtct tggatctggc
      901 ggcagacctg tcgttcgcgc ccaacggcac cgggcgcggc ctcagcaccg cgcgcacgca
      961 aaccctgggc ttcgtggtcc gccgcaaggc gtttcccacg gtgcaggatc acttctattt
     1021 gcagatcatg cacggcgtgg aagaggaggt cgcgcgcaat ggctaccatg tcatcctatc
     1081 ggcggtcggc gcccccgccg gagcgaacac gatgaacggc gccaacgacc tgtcgctcgt
     1141 ccgcgaaggc cgggtggatg gcctgatcct ggccggcccg gacatcgaca gcagcctgat
     1201 cctggaactc cagcacaacg gggtgccgct ggtgctggtc gacaacgcat tgcggcaaac
     1261 gcccatcgat tgcgtgctga acgacgaccg cgagggcgcc ttcgccgccg tcagccacct
     1321 gatcgaacac ggctacgagc gcatcgcctt catcggcggc ccctccacct ggatcagcac
     1381 ggtggagcgc cgggcgggct tcgaggacgc gctggccgcg cacaacaaga ccgcctgcgc
     1441 catcgtccac cagcgcgaaa cgacggtcga gaccggctat gccgccatga tcgaattgct
     1501 ggagcaggac acacggccca acgccgtctt cgcggtgaac gacagcatgg ctttcggcgc
     1561 catgcgcgcc gcgcgcgaga acggcctgac cgtgcccggc gacctggcgc tcgccggctt
     1621 cgacgacatc gacgcggcgc agcataccac gccacccctg accaccgtgc gcatcttcaa
     1681 ggaacgggtg ggcgaggtcg ccgcgcggcg gttgctggac cgcctcaccg gcccggcgac
     1741 gccgccccag cgcacctatg tcggcaccga actcattatc cgccagtcgt gcgggtgccc
     1801 acccgccccc atccgaaggg aggtggtcag cgccgccgat accccgtaat gccgcccggc
     1861 ccgtagtcgt ggcggctcgc cctcagcccc caatcgcgtc caggttagtt gcgaaggagg
     1921 aaagatcgtg gaacgtatac accgcatgtt cgccctgctg gcgacccttg ccttgctgct
     1981 gccgctgctg gcggcctgtg gcggcgaaac ccccgccacc acggcccccg ccgcagccac
     2041 aaatacgacc gcgccggccc ccgccgccgc ggcgaccgat acgcccgcgt ccgccgcagc
     2101 cgcgcccacc aacaccaccg cccccgccgc cggtaacgcg ggcggcgaaa aggtcacgct
     2161 gaccatggcg acctgggccg gggccgatga gtccaaggaa ctccaggcca tcatcgacaa
     2221 agtcaacgcc gccgccaccg actaccagat cgtctacaac ccggcccctg ccgact
//
LOCUS       DASUYC010001196         3322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_222349,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001196 DASUYC010000000
VERSION     DASUYC010001196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222349"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="VF276_19575"
     CDS             complement(<1..646)
                     /locus_tag="VF276_19575"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX6609110.1"
                     /translation="MKDTSILSYPPRTDTPGASPARWPVYLAILTGACLVALVLSFTG
                     QPIFEILNRWIQPDDREMGSALFSVYYLVIGLLPALWWPRRLGWQVGRVRREWRLLAG
                     LLVLVSVLSIGFILLAGPIPFHNFPWTQFTVAPLSEEAVFRGVFFAAILALLRRAGAG
                     ERSTVLAIWFSAIAFGLAHSSNYLYFPAGFVTFQIGEATLMGALLAYVRVKTDSL"
     gene            complement(696..1748)
                     /locus_tag="VF276_19580"
     CDS             complement(696..1748)
                     /locus_tag="VF276_19580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6609111.1"
                     /translation="MQIEALPPQKVAVRYQTVLAAGIEFLIALFVLVVYTVLHESGHA
                     LVGLAFGGTITAFNTNFLNLTAHVGIEGNFSILQQCLVAVAGVSFPLLVWAAFVYSTP
                     RQANIVLDSLKLISSLAVLNTLLAWIVIPLLYLGGGRPGDDSTNFLNYSGAPPLLVTG
                     VALLLYAAGWRFYVARVGGIAGARRLIAHYRAASDAVVDRQALRALRILVVAGVVVAG
                     ASVALNWYLTRTNPLGLPAGYREVATLSLEATGYTEASVYQFTLDQPATAGFYILLQD
                     IQAGPVEITLVGPSGYRNTFLKFRSDFNAMGRGTVHPQGLALEPGAYAIQLTVPRTPG
                     RIVISTKLDGAEPGNR"
     gene            complement(2078..2362)
                     /locus_tag="VF276_19585"
     CDS             complement(2078..2362)
                     /locus_tag="VF276_19585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6609112.1"
                     /translation="MGHACAEAGHRFDQPADPCHLVVLSVPTAHHLRAALGHIGRAGI
                     RCAVFHEPDDQLGDTAACTEPIPGAYRRVFRRFLLWSGDTLPSARGPPVR"
     gene            complement(2436..2897)
                     /locus_tag="VF276_19590"
     CDS             complement(2436..2897)
                     /locus_tag="VF276_19590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6609113.1"
                     /translation="MNTLPRAVTARFFPDPESYTALRRQWWLLLTSDRKHELTAAHHL
                     LYLALLGKDWRRGFTPITNTRKLENGAFTGWALFRALGSIAWPGDESALLAPFAGTVT
                     PGMLQSLRALLPQASPYACRPEDFAAGFPWDAYTVPEALPAASQPEDLSRG"
BASE COUNT          692 a         1033 c         1116 g          481 t
ORIGIN      
        1 agaggctatc ggtcttgacg cggacatagg ccagcagggc gcccatcagc gttgcctcgc
       61 cgatctggaa ggtgacgaag ccggcgggga aatacagata attcgagcta tgggcgagcc
      121 cgaaggcgat ggcgctgaac cagatcgcga ggacggtgct gcgttcgcct gccccggccc
      181 gccgcagcag ggcgaggatg gccgcgaaga acacgccgcg gaacacggcc tcctcgctca
      241 ggggcgccac ggtgaactgc gtccagggaa agttatggaa cgggatgggt ccggccagca
      301 ggataaagcc gatggagagc acgctgacca agaccagcag cccggccagc aaacgccatt
      361 cccggcgaac ccggcccacc tgccacccca accgccgcgg ccaccagagg gcgggcagca
      421 ggccgatcac caggtagtag acgctgaaca gggccgagcc catctcgcgg tcgtccggct
      481 ggatccagcg attcaggatc tcgaagatcg gttgccccgt aaaggacaac accagcgcca
      541 cgaggcaagc gccggtcagg atggcgagat agaccggcca ccgcgccggc gacgcgccgg
      601 gcgtatcggt acgcggcgga tacgatagta tggaggtgtc cttcatcggc ggcatcccct
      661 atcgacgagc cgcgcggcgc acagcgcggt agacctcagc gatttcccgg ctcagcgcca
      721 tcaagttttg tggagatcac gatccggcca ggtgtccggg gcaccgtgag ttgaattgcg
      781 taagcccctg gttcgagcgc taacccctgc ggatggacgg tcccgcgccc cattgcgttg
      841 aagtcggagc gaaacttcaa gaaggtgttg cgatagccgc tcggccccac cagcgttatc
      901 tcgaccgggc ctgcttgaat atcctgcaag agaatataaa aaccggcggt cgcaggttga
      961 tcgagggtaa actgatacac actggcctct gtataccccg tcgcctccag ggaaagcgta
     1021 gcgacttctc tgtaacctgc cggcagcccc agcggattgg tccgggtcag ataccagttc
     1081 aacgcgaccg aggcgccggc aacgaccacg cccgccacca ccaggatgcg caacgcgcgc
     1141 aacgcctgcc ggtcaacaac cgcatcggac gcggcgcgat aatgcgcgat caggcgccgg
     1201 gctcccgcga tcccgcccac ccgggcgacg taaaagcgcc aaccagccgc atagagaagc
     1261 agcgccacgc ccgtcacgag cagcggcgga gcgcccgaat agttgaggaa attcgtggaa
     1321 tcatcgcccg gtcgcccacc cccgagataa agcagcggga tgacgatcca ggccagcagc
     1381 gtgttgagca ccgccagaga gctgatcagc ttcagcgagt cgaggacgat gttcgcttgg
     1441 cggggcgtag aatagacgaa ggcggcccag accagcaacg gaaacgacac gccggcaaca
     1501 gccaccaaac actgctggag aatcgagaag ttgccctcga ttcccacatg cgcggtcaga
     1561 tttaggaagt tcgtgttaaa tgccgtgatg gttccgccga acgcgagccc gaccagcgcg
     1621 tgaccactct cgtgcaggac ggtatagacg acgagcacaa acagggcgat caaaaattcg
     1681 atcccggcag ccagcaccgt ttgatagcgg acggctacct tttgcggagg aagcgcctcg
     1741 atctgcatgg agtccctccg ggatgtaccc ggcgccgccg gaacccgatc tgccgccagg
     1801 ccctacatca tcgccctacc catgccggat gcggcagacg ctacagcggt ccggctatta
     1861 gcgtactgcg cacaggtcaa catcaggggg tattgtggtg acaattttgt gaattggcac
     1921 atcgccggcg gggcatccgg caagagcggc acgccgcacg gcgggagcag gggatcgatt
     1981 gagcaggcca cgcgcgacac cggctcccgc caggccgtcg cgcgtgcccg gttcatatgc
     2041 aagacgagat agcgatcccg gctgtgacag aaagcggtca acgcaccggc ggaccgcggg
     2101 cggagggtag cgtatcgccg gaccagagca ggaagcggcg gaagacgcgc cggtaggcgc
     2161 cggggatcgg ctcggtacag gcggcggtat cgcccagttg gtcgtcgggt tcgtggaaga
     2221 cggcacagcg gatcccggcc cggccaatgt ggccgagggc cgcgcgcaga tggtgcgcgg
     2281 tgggaacgct gaggactacc aggtggcagg ggtcggcggg ctgatcgaag cggtggcctg
     2341 cctcggcgca cgcatggccc acctggacga tctgatcggc gagcggaatg tccgcgcgga
     2401 ccaggacgta gatgtagcga ggggcaatct acgtatcagc cacgggaaag gtcctcgggc
     2461 tgcgacgcag cgggcaacgc ctcgggcacg gtgtaggcgt cccaggggaa gcccgccgcg
     2521 aaatcctccg gacggcaggc ataaggactc gcctggggca gcagcgcgcg cagactctgc
     2581 aacatccccg gcgtcaccgt cccggcgaag ggcgcgagca acgccgactc atcgcccggc
     2641 caggcgatgc tgccgagcgc ccggaacagc gcccacccgg taaaggcacc gttctccagc
     2701 ttccgcgtat tggtaatcgg ggtgaaaccc cggcgccaat ccttgcccag cagggccagg
     2761 tacagaagat ggtgcgcggc cgtaagctcg tgcttgcggt cggaggtcag cagcagccac
     2821 cactgccggc gcagggcggt atacgattca ggatcaggga aaaaccgggc cgtgacggcc
     2881 cgcggtaacg tattcatcag ttgtatctcc atatcgttga gggtcaatga cgacaacagc
     2941 agcaggcggg cgcctgccgg gaagatacaa ccgggggacc tctagtttgt tggcgtagta
     3001 cctaccacat gggaaactcc ttgccggtgc gccatgcgag aatcgaactc gctgcaggcg
     3061 ggtttagagt ccgccactca tccaacgagt atatggcgcg cgataagcct attctactca
     3121 ggagtattcc cgccgtcaat aggccgaaag actcattctc gccggcgcgg cgggttcgct
     3181 aactgggaat cgtcgcgcgg gcttgcggcg aacgcgccgg gatgcgggct agccagcggc
     3241 gggtggcggc gggggacggg aggcggatca tctgcaggtg ggcatattcg ggctgccggg
     3301 gcagtgtgct atactcgctg cg
//
LOCUS       DASUYC010001197         2462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1840457, whole genome shotgun sequence.
ACCESSION   DASUYC010001197 DASUYC010000000
VERSION     DASUYC010001197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1840457"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2002)
                     /locus_tag="VF276_19595"
     CDS             complement(<1..2002)
                     /locus_tag="VF276_19595"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6609114.1"
                     /translation="MEFLEREQYADTLGAVLREAVGGAGRTVLIGGEAGIGKTTLVER
                     FIAEQGRAARVLWGACDALLTPRPLGPLHDIMRQAPAELGTLLDLGKDWLDIASALLA
                     WLERGAVPTVIVVEDAHWADEATLDLLRFLGRRMPKTRALLVVTYRDDELAPQHSLWR
                     VLGDLPARATIRLALPRLSEAAVDRLAQQAGRPTGGLYVATGGNPFFVTEVLAHGAEG
                     LPATVRDAVLGRAARLSPPARQVLDLASIVPGAIEPGIVAAVCGPSAPQIEECAASGL
                     LLLRDDRLAFRHDLARRAVEETLPATRARELHAQVLAALTGDGMEPVPLARLVHHATQ
                     AGHAPSVLRFAPLAARQASGAGAHCQAADHYATALRYAARLPPAERAELLEGRSFECY
                     LMADSDAAIQARQEATDIWRRLDRPEREGDGVRWLSRLFWQAGNKQEADRYADEAIAI
                     LEPLAPGPALAMAYSNRAQLHGLAEEHAAALGWGRRALALAWELGETEIMVHALTNIG
                     ADELRLGDEAGRAKLETALATALAHELHDHVARAYANLACAAIEGRDYARGLDYLEKG
                     LAYATARDLDTYGPYLRGWRARARFEQGQWRAAEEDARRALRDPPGAAVIPLPALITL
                     GHLYARCGEPTAAALLDSARTIAIRTGEPQRLGPLAAARAEAA"
BASE COUNT          368 a          882 c          866 g          346 t
ORIGIN      
        1 acgccgcctc cgcgcgcgcc gccgccagcg ggccgagccg ttgcggctcc ccggtgcgta
       61 tggcgatggt acgggcggaa tcgagcaggg ccgcggcggt aggttccccg caccgggcgt
      121 acaaatggcc cagggtgatc agcgccggga gcgggattac ggcggcgccc ggcggatcgc
      181 gcagcgcccg gcgcgcatcc tcttccgccg ctcgccactg gccctgctcg aaccgcgcgc
      241 gggctcgcca cccgcgcaga taagggccat aggtgtccag gtcgcgcgcc gtggcatacg
      301 ccaggccttt ctccagatag tccaggcccc gcgcgtagtc gcgcccctcg atcgccgcgc
      361 aggcgaggtt ggcataggcc cgcgccacat gatcgtgcaa ctcgtgcgcc agcgcggtgg
      421 cgagcgccgt ttccagcttg gcgcggcccg cctcatcgcc gagccgtaac tcgtccgcgc
      481 cgatattggt cagcgcatgg accatgatct cggtctcgcc cagctcccag gccagcgcca
      541 gggcccggcg gccccagccc agcgcagcgg cgtgttcttc ggccagcccg tgcaattgcg
      601 cgcggttgct gtaagccatc gccagcgcgg gccccggcgc gagcggctcc aggatcgcga
      661 tggcctcgtc ggcgtagcgg tcggcctcct gtttgttgcc ggcctgccag aagagccgcg
      721 agagccagcg gaccccgtcg ccctctcgtt ccgggcgatc caggcgccgc cagatatcgg
      781 tcgcctcttg ccgcgcctgg atcgcggcat cggaatcggc catcagatag cactcgaacg
      841 agcggccttc gagtagttcg gcccgttccg cggggggcag gcgcgcggcg tagcggagcg
      901 ccgtggcgta atggtcggcg gcctggcagt gcgcgcccgc cccgctggcc tggcgcgcgg
      961 ccagcggcgc gaagcgcaac acgctcggcg cgtggccggc ctgggtggcg tggtgtacta
     1021 atcgggccag cgggaccggc tccataccgt cgccggtcag ggcggccagc acctgggcgt
     1081 ggagttccct ggcccgcgtg gccgggagcg tttcctcgac cgcgcgccgt gcgagatcgt
     1141 gacgaaacgc gagccgatcg tcacgcagca gcagcaaccc gcttgcggcg cactcctcga
     1201 tctggggcgc agacggaccg cagaccgcgg ccacgatccc tggctcgatg gctcccggca
     1261 caatggacgc caggtccagc acctgccggg cgggcgggga gagccgcgcc gcccgcccca
     1321 gtacggcgtc gcgcaccgtc gcgggcaagc cttcggcgcc atgcgccagc acctcggtga
     1381 cgaagaaggg gttgccgccc gtggcgacgt acaggccgcc ggtggggcgc ccggcctgct
     1441 gcgccaggcg atcgacggcg gcttcggaga gccggggtag cgccagccgg atcgttgccc
     1501 gtgcgggcag atcgccgagg acccgccaca aggagtgctg cggcgccagt tcgtcgtcgc
     1561 gataggtgac cacgagcagg gcgcgcgtct tgggcatgcg ccggcccaga aaccgcagca
     1621 ggtccagcgt ggcttcgtcc gcccagtggg cgtcctcgac gacgatcacg gtgggtaccg
     1681 cgccccgttc caaccaggcc aggagcgcgg aggcgatatc cagccagtcc ttgcccaagt
     1741 cgagcagggt gccgagttcg gcgggggcct ggcgcatgat gtcgtgcaac gggccgaggg
     1801 ggcggggtgt gagcagcgca tcgcaggcgc cccagagcac gcgggcggcc cggccctgct
     1861 cggcgatgaa gcgctcgacc agcgtcgtct tgccgatgcc ggcctcgccg ccaatcagca
     1921 ccgtgcggcc ggccccaccg acggcctccc gcaagaccgc gcccagggtg tccgcatatt
     1981 gttcgcgttc gagaaactcc atcgcccgct caccatgcgc gtcgtccggc gcctgcggat
     2041 aggtagccgc gcctggcccg ccgctcgcca gagcggatca gaccggcgcc ttgctcctac
     2101 tatagcacac tggctgcaaa atagggagtg gcccccgcaa aatagggatc tctcccgatg
     2161 tgccgtggcg gcgcatggcg tatcgtggta atagcagcgg ccaccatggc acagcccatc
     2221 gcggcttccc gcaggggcaa gcagtgggcg catccgttaa ggcctggtgc aagcgagggt
     2281 ccgctgtaat ccgtcgggag cctggacggc gaatacacta cctgcctgtc cggcaacgac
     2341 cgtcctgaaa gaaataacat gactacgact atgtctgcac tccagttggc cctgatcaag
     2401 ggccggcaac aacaaacctg ggccgccggc gactacggca tgatcggcac gaccctgatc
     2461 ct
//
LOCUS       DASUYC010001198         7447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_722662,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001198 DASUYC010000000
VERSION     DASUYC010001198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 7447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7447
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722662"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VF276_19600"
     CDS             complement(<1..329)
                     /locus_tag="VF276_19600"
                     /inference="COORDINATES: protein motif:HMM:NF015414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX6609115.1"
                     /translation="MTEQVVYLTPEGKAELESKLEFLRTTRRREVAERIRGAKDDGDI
                     SESGEYEDAKREQAFLEGEIRSIEQTLRYGRVIENNGTTETVGVGRTVYVKDAEGVEE
                     NWRIVSPA"
     gene            complement(674..1081)
                     /locus_tag="VF276_19605"
     CDS             complement(674..1081)
                     /locus_tag="VF276_19605"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6609116.1"
                     /translation="MNLGFVVLYVDDMDKVKAFYTVLLGMSVVPEASGPHFLTLRPAG
                     GSLVALQDKAGARFPPAHETGSGSVELSFEVADVDGTWQHWKDQGVEIVSDPVDLPFG
                     RYFLAKDPEGHYHSVYRFAQQPAIPAESTGQAG"
     gene            complement(1230..4661)
                     /locus_tag="VF276_19610"
     CDS             complement(1230..4661)
                     /locus_tag="VF276_19610"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX6609117.1"
                     /translation="MPLTSRWPAIQQLAAAQRAQMLTVAAAPPRQLVEQSLAAHGLDV
                     FPLPPGDPLLGGARAVFDGEFVAYDGGLPATAATFSLAHELGHKLLHDEQARCAAADI
                     DEECAALGLDAAPGGVETYSPRQQRELEANAFAAAFLMPAAELRAGFLAGLSYPELAA
                     RCGVSESAILNTLSATLLAAPPSSPAPAAPAPPAEPPLRLDPSQEAAATVAEGPVLID
                     AGPGTGKTRTLIERVLFLLRNGVAPQAILALTFSNRAAGEMRERLRLAAPAAADEITV
                     GTFHAFCLQFLQEHAAEAGLPPDFTLVDSVQAAAWLEPHLPEMALQHYTTLANPARSL
                     PDILDAISRARDELKDAADYADLAARARAAADPEDAKAVEQAERWAEVARVYAVYERV
                     LEQHGALDFGALLLRTVRLLDTHPALLFRLQRRYSQILVDEYQDMNRASGVLLSRLAG
                     DGRGLWVVGDLRQAIYRFRGASPANIDKFETDFPRGRRLRLGVNYRSDPAIVGLFRQL
                     GREMKVGDRATAQWDAHRAAQPGPRIWQATAASGVAEAAGIAEAVRTRHEAGRYYREQ
                     VVLCRTHGQAALVARALEAADVPTLYLGNILEREEVRDVLALVSLAAEGDGRALLRAA
                     RLTGHAVDRGACARLVRHARAAGISFPRALNLAADAGLDAETAAACAALAAIIADAGR
                     YGDAAGFLARYLFGPASPLRALLADGSARAVARLLALGQLLALARSFQAPSASAAAGE
                     DSAPADPAAPLREFLAHVRRLYAAKEVPGRTPPGGEDVDAVRVLTVHASKGLEFPVVY
                     LPGLAAGRFPFREMWEPCPAPPGLIDGAEAGDHDMEETCLFFVALSRARDELILSHAE
                     SYGKRKAKDSPFWTMIEPFFQETPVPVLQWQAAEDPAPPAPPAPSGEDRLTLPVEDLE
                     LYLRCPRQYEYSRALRLDDSDESDGYPRFYRVVQTVLGRLREDHERGALPAHEGVVLD
                     LLAGAWETGGPQGHVHEAIYAEAARHVVVATWQRLSATAPADQAPIELSLPLGGATVR
                     ARYDLAERRSDQGVRLVRTRVGKPRDEDRKALRLALLRAAARHTLGAEVPIAIELEYL
                     GTGETVEVAESGRYEQQRLDKLEGAVLGIRARHFPPAPAETDECLRCPFWIICPA"
     gene            complement(4669..5271)
                     /locus_tag="VF276_19615"
     CDS             complement(4669..5271)
                     /locus_tag="VF276_19615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6609118.1"
                     /translation="MNQQHHEPQAAESLRQVQLAFLDALDRGERADGWLQRYPQHAAA
                     LIDLAQARDLEAALPPPTAAEVAAVAAIARRTLAVTTGAALSLSERARQAGLSIRDLA
                     ARVGLTSDILFKIDRRVVRPETVPGALVRELAAALGCTAAALRAGLTGGPVTAGALYH
                     AKQAPQVGQQTFAEAVAASVALPPADRRRWLEAAAHPDAE"
     gene            complement(5268..6044)
                     /locus_tag="VF276_19620"
     CDS             complement(5268..6044)
                     /locus_tag="VF276_19620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6609119.1"
                     /translation="MPELIEQGSAEVPPPGGLGLSRNYRRRAEVEAEIVALPAATDGG
                     LLPALQAAGAVETLVHGIRRLVKAGRHAEADQVAQALIERVTPLITRIAAGQVVLAED
                     REDLVQTVLVQMWQEVYDTAPQHEFWEVHFAHMLRVACGDAAERLRRPRDHERAFKRG
                     QSEDGDPWSEEDTIADPQAGEPALLVPEALAQLDGNVRRALYLKTLGYKERSKDVGEP
                     TISSLLRVSDRTVRNYLRTAEEVLRPWLERGLLESGQVQT"
     gene            complement(6359..>7447)
                     /locus_tag="VF276_19625"
     CDS             complement(6359..>7447)
                     /locus_tag="VF276_19625"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEX6609120.1"
                     /translation="DSPPFLSTLFGYFQDLRTDVNGVQPSGVYSSVVGTAPNRQYVLR
                     WHATYFGNASNEVDFEVVMNENSPTISVIYGQVPANANDAGVGIQLNDGQYTQFSCSG
                     NPPLTAGLRVDYVPVSCNAPTATVTPQNTPTATQTSVPLATQTSVPGTATATATPVNC
                     PLPFTDVDQFNPFYQYIQCLYCRGIISGYSDNTFRWGNDVTRGQVSKIIANSAGLNDT
                     VSGQTFTDVDAANPFYVYIERLYQHGFISGYDTAANCPTGTPCFRWELPVTRGQLAKI
                     DANAAGYNETPTGQTFRDVPAANPFYVFIERLSLHGVISGYDCGGPGEPCPGLYYRPF
                     NNITRGQVSKVASQTFFPNSCAPAATRP"
BASE COUNT         1053 a         2522 c         2702 g         1170 t
ORIGIN      
        1 gcggggctga cgatacgcca gttctcctcc acgccctcgg catccttcac gtagaccgtc
       61 cggcccacgc caacggtttc ggtggtgccg ttgttctcga tcacgcggcc atagcgaagg
      121 gtctgctcga tgctgcggat ctcgccttcg aggaacgcct gctcgcgctt ggcgtcctcg
      181 tattcgccgc tctcgctgat atcgccgtca tctttcgcac cacggatgcg ctccgcgacc
      241 tcgcggcggc gcgtagtgcg caggaattca agtttagact ccagttcggc tttgccctcc
      301 ggagtcaggt ataccacctg ctcagtcata gctgctcact tcctacccct cactcttagc
      361 ggaggtgttg tgctacattg ccaacacgcc cggcgacggt ccgggggata aataaatgcc
      421 cccgcacgta gcgggggtgc aaaagctgcg cgaagaccct tttgaccggt gctgggtccc
      481 tctggaaaca gcttacagat gttatagcaa aaatcgcggc attttgcaag tagcccatta
      541 gtccttgatc ggtatgtgaa cgaattgtaa caaaaggacg ttcggcctcc cgccgcctgc
      601 gcttgcaccc aggccggcgc ggcccccacc ggttatggcg ggggccgcgt cacgtgctgc
      661 tcgggccgcc cgcctagccg gcctggccgg tgctttccgc ggggatcgcc ggttgctggg
      721 cgaagcgata cacggagtga tagtggccct ccggatcctt cgccaggaaa tagcgcccaa
      781 acggcagatc caccggatcg ctcacgattt cgacgccctg gtccttccag tgctgccatg
      841 tgccgtccac gtcggcgacc tcgaagctca gttccacgct gccggacccc gtctcgtgcg
      901 ccggcgggaa gcgcgccccg gccttgtctt gcagggcgac cagtgagccg cctgcggggc
      961 gcagggtcag gaagtgcggg ccggaagcct ccggcacgac gctcatgccc agcagtacgg
     1021 tgtagaacgc cttcaccttg tccatgtcat ccacgtacag caccacaaag cccaggttca
     1081 tcgtctgctc tccttctcgg ggtatcgcgg cggttgcctc ttcactggcc gccggtatct
     1141 tgattatagg agagccctgt gacagcatta tgtcagcagc gtttcgcggt ttgtggcttg
     1201 ttttgcgcgg gaagagggcg atccccgcgc taggccgggc agatgatcca gaaggggcag
     1261 cgcaggcact cgtcggtttc ggcgggagca ggcgggaagt gccgggcgcg gatgccgagc
     1321 acggcgccct ccagtttgtc cagccgctgc tgctcgtagc gcccggactc agccacctcc
     1381 acggtctcac cggtgcccag atactccaac tcgatagcga tgggcacctc ggcacctaag
     1441 gtgtggcggg ccgcggcgcg cagcagcgcc agccggagcg ccttgcggtc ctcgtcgcgc
     1501 ggcttgccga cgcgggtgcg caccaggcgc acgccctgat cagaccggcg ctcggccagg
     1561 tcgtagcggg cgcgcaccgt ggccccgccc agcggcaggc tgagctcgat cggcgcctgg
     1621 tcggcaggcg ctgtggcgct caaccgctgc caggtggcga cgacgacgtg ccgcgccgcc
     1681 tccgcgtaga tggcctcgtg gacgtggccc tgcggtccgc cggtctccca ggcccccgcc
     1741 agcaggtcga ggaccacccc ttcgtgcgcg gggagcgcgc cacgctcgtg gtcctcgcgc
     1801 agacggccca ggaccgtctg caccacgcgg tagaagcgcg ggtagccgtc gctttcgtcg
     1861 ctgtcgtcga ggcgtagggc gcggctgtat tcgtactgcc gcgggcagcg cagatagagt
     1921 tccaggtcct cgacgggcag cgtcagccga tcctcgccgg aaggggccgg gggcgcgggc
     1981 ggggccgggt cctctgcggc ctgccattgc agcaccggca ctggcgtctc ctggaagaac
     2041 ggctcgatca tggtccagaa gggcgaatcc ttggcttttc gcttgccgta gctttcggcg
     2101 tggctcagga tcaactcgtc gcgggcgcgg ctcagggcca cgaagaacag gcaggtttcc
     2161 tccatgtcgt ggtcgcctgc ctccgcgccg tcgatcaggc cgggcggcgc ggggcacggc
     2221 tcccacatct cgcggaaggg gaagcgcccg gccgccagcc ccggcaggta gaccaccgga
     2281 aactccagcc ccttgctggc gtggacggtg aggacgcgca cggcgtccac gtcctcgccg
     2341 ccgggcggcg tgcggcccgg cacctccttg gccgcgtaga ggcggcgcac gtgggccagg
     2401 aactcgcgca acggggcggc agggtcggcg ggcgcgctat cctcgccggc agcggcgctc
     2461 gcggacggcg cctgaaagct ccgcgccagg gccagcaact ggccgagcgc cagaagccgg
     2521 gccaccgcgc gggcgctacc atcggccagc agcgcgcgta ggggtgacgc cgggccgaac
     2581 aggtagcgcg ccaggaaccc cgcggcgtcg ccgtaccgcc cggcatcggc gataatcgcg
     2641 gccagcgcgg cgcaggcggc agcggtctcc gcgtcgagtc ccgcgtccgc cgccaggttc
     2701 agggcgcgag ggaaggagat ccccgccgcg cgggcgtgcc ggaccaggcg ggcgcaggcg
     2761 ccacggtcca cggcgtggcc ggtcagccgg gcggcgcgca gcagggcgcg cccgtcgccc
     2821 tcggcggcga gcgagaccag ggccagcaca tcgcgcactt cctcgcgctc caggatgttg
     2881 cccaggtaga gcgtgggcac gtcggcggct tcgagcgcgc gggccaccag ggccgcctgc
     2941 ccgtgcgtgc ggcagagcac cacctgttcg cggtagtacc gcccggcctc gtgccgcgtc
     3001 cgcaccgctt ccgcgatgcc cgccgcctcc gccacgccgg aggccgccgt ggcctgccag
     3061 atacgcggcc ccggctgcgc ggcgcggtgg gcgtcccact gcgccgtggc ccggtcgccc
     3121 accttcatct cgcggcccag ttgcctgaac aacccgacaa tggccgggtc ggaccggtaa
     3181 ttgacgccga gacgcaggcg ccgtccgcgg gggaaatcgg tttcgaactt gtcgatattg
     3241 gccggggatg cgccgcggaa gcggtagatc gcctggcgca agtcgcccac cacccagagc
     3301 ccgcgcccgt cccccgccag ccggctcagc agcacgccgc tggcgcggtt catgtcctgg
     3361 tactcgtcga ccaggatctg gctgtagcgg cgctgcagcc ggaacagcag ggccgggtgg
     3421 gtgtccagca ggcggacggt gcgcagcagc aatgcgccga agtcgagcgc gccgtgctgc
     3481 tccagcacgc gctcgtagac ggcgtagacg cgggccacct cggcccagcg ctcggcctgc
     3541 tccacggctt tcgcatcctc gggatcggcg gcggcgcggg cgcgggccgc gagatcggcg
     3601 taatccgcgg cgtccttcaa ctcgtcgcgg gcgcggctga tggcgtcgag gatgtcgggc
     3661 aagctccgcg ccgggttcgc cagcgtggtg tagtgctgga gggccatctc cggcaggtgc
     3721 ggctcgagcc aggcggcggc ctgcacgctg tccaccaggg tgaagtcggg cggcagtccg
     3781 gcctcggcgg cgtgttcctg aaggaattgc aggcagaaag cgtggaacgt gccgacggtg
     3841 atctcgtcgg cggcagccgg cgcggccagg cgcaggcgtt cgcgcatctc gcccgccgcc
     3901 cggttggaga aggtcagggc caggatggcc tggggcgcca cgccgttgcg cagcaggaac
     3961 agcacccgct cgatcagggt gcgcgtcttg cccgtgcccg gcccggcgtc gatcaacacc
     4021 ggaccttcgg ctaccgtggc cgccgcttcc tgcgagggat cgagccgcag gggcggctcc
     4081 gcgggcgggg cgggggcagc gggcgccgga ctggaaggcg gcgcggccag caaggtggcg
     4141 ctcagcgtgt tgaggatggc cgactcggac acaccgcagc gcgccgccag ttcgggatag
     4201 ctcagcccgg ccaggaaccc ggcgcgcaac tcggcggcgg gcatgaggaa ggccgcggcg
     4261 aaggcgttgg cctcgagttc gcgctgctgg cgcgggctgt aggtctcgac cccgcccggc
     4321 gcggcatcga ggccgagcgc ggcgcactcc tcgtcgatgt cggcggcggc gcaacgggcc
     4381 tgctcgtcat gcagcagctt gtggccgagt tcgtgggcca ggctgaaagt ggccgcggtg
     4441 gccggcaggc cgccgtcata ggcgacaaac tcgccgtcga aaacggcccg cgcgccgccc
     4501 agcaggggat cgccgggcgg cagcggaaac acgtcgaggc cgtgcgccgc taacgactgt
     4561 tcgacaagct gccgcggcgg ggcagcggca acggtcagca tctgggcgcg ctgggcggcg
     4621 gcgagttgtt ggatggcggg ccagcggctg gtcagcggca tggggcggtc actcggcgtc
     4681 ggggtgggcg gcggcctcca accagcgccg gcggtcggcg ggcggcagcg ccacgctggc
     4741 cgcgacggcc tcggcgaacg tctgttgacc cacctgcggc gcctgcttgg cgtggtagag
     4801 cgcgcccgcc gtgaccgggc cgccggtcag cccggcgcgc agcgcggcgg cggtgcagcc
     4861 gagcgcggcg gcaagttcgc gcaccagcgc gccgggcacc gtctcgggcc gcaccacgcg
     4921 ccggtcgatc ttgaacagga tgtcgctggt cagccccacg cgggcggcca ggtcgcggat
     4981 ggagagaccg gcctggcggg cgcgctcgct caggctgagc gccgcgccgg tggtcacggc
     5041 cagtgtgcgg cgggcgatgg cggcgacggc ggccacctcg gcggcggtgg gcggcggcag
     5101 cgccgcttcc aggtcgcgcg cctgggccag atcgatcagc gcggcggcat gttgcgggta
     5161 gcgctgcaac cacccatcgg cccgttcgcc ccggtccagc gcgtccagga aagctaattg
     5221 cacctggcgg aggctctccg ccgcttgcgg ctcatgatgt tgctggttca tgtttgtacc
     5281 tgtccgcttt ccagaagccc acgttccagc caggggcgca agacctcttc cgccgtgcgc
     5341 aggtagttgc gcaccgtgcg gtcgctcacg cgcagcaggc tgctgatcgt cggctctcca
     5401 acgtctttac tgcgttcctt atagcccagc gtcttcagat agagcgcccg ccgcacattg
     5461 ccgtcgagtt gggccagggc ctcgggcacc agcagcgcgg gttcgccggc ctgcgggtcg
     5521 gcgatggtgt cttcctcgct ccacgggtcg ccgtcctcgc tctggccacg cttgaaggcc
     5581 cgttcgtggt cgcgcgggcg gcgcaggcgc tcggcggcgt cgccgcaggc cacgcgcagc
     5641 atatgcgcga aatggacctc ccagaactcg tgctgcggcg cggtgtcgta gacctcctgc
     5701 cacatctgca ccagcaccgt ttgcaccagg tcttcgcggt cctcggccag gaccacctgg
     5761 ccggcggcga tgcgggtgat gagcggcgtc acacgctcga tcagggcctg ggccacctgg
     5821 tcggcttcgg cgtggcgccc ggctttcacc aggcggcgga tgccgtggac cagggtctcg
     5881 acggcgccgg cagcctgcag cgcagggagc aacccgccgt cggtcgcggc aggcagcgcc
     5941 acgatttcgg cttccacctc ggcgcggcgg cggtagttcc ggctgaggcc cagtccgccg
     6001 gggggcggga cctccgcgga tccttgttcg atcaactcgg gcactgtgct ggctggcctc
     6061 ctcttgggat gcgggtctgc ggtgctctta cgtttcctat cgccagggga tgggaaatgc
     6121 ggaaagcggg tacataccgg gtacatccag ggtttgcgga caagttgttc caccccatta
     6181 tatcaaatcc ggcctggcgg ggaaaaggcc ggggatcctc cgcgcgttcc gggtaggggc
     6241 gcccgcacgg cagagacgaa ggccggcccc ctgagcagga gccggccttc ggggttgccc
     6301 cgcggcgcgg gccgtgggcc ggcccgcatc cgcgaggtcc tacgatgcgc ttgtccggtt
     6361 acggacgggt ggcggccggc gcgcaggagt tcgggaagaa cgtctgcgag gcgaccttgc
     6421 tcacctgccc gcgggtgatg ttgttgaaag gccggtagta gaggccgggg cacggctcgc
     6481 cggggccacc gcagtcgtag ccgctgatga cgccgtgcag cgacaaccgt tcgatgaaca
     6541 catagaacgg attggcggcg ggcacatcgc ggaacgtctg gccggtgggc gtctcgttat
     6601 agcccgccgc gttggcgtcg atcttggcga gctggccgcg cgtcaccggc agttcccagc
     6661 ggaagcaggg agtgccggtg gggcagttcg ccgctgtatc gtagccgctg ataaagccgt
     6721 gctgatacaa ccgctcgatg tagacataga acgggttagc cgcgtccacg tcggtgaacg
     6781 tctgcccgct gacggtatcg ttcagcccgg cggagttggc gatgatcttg ctcacctggc
     6841 cgcgggtcac gtcgttgccc cagcggaagg tgttatcgct gtagccggag atgatgccgc
     6901 ggcagtacag gcactggatg tactggtaga acgggttgaa ctgatcgaca tcggtgaacg
     6961 gcagcgggca gttcaccggc gtcgccgtcg cggtcgcggt ccccggcacg ctggtttgcg
     7021 tcgccagcgg cacggacgtt tgcgtcgcgg taggcgtgtt ctgcggcgtc accgtcgcgg
     7081 tgggcgcgtt gcaggacacg ggcacatagt ccacgcgcag gccggcggtc agcggcgggt
     7141 tgccgctgca cgagaactgc gtgtactggc cgtcgttgag ctggataccc acgcccgcat
     7201 cgttggcgtt cgccggcacc tggccgtaga tgaccgagat ggtcggcgag ttctcgttca
     7261 tgaccacttc gaagtccacc tcgttggagg cgttgccgaa gtaggtcgcg tgccagcgca
     7321 gcacgtactg gcgattcggc gcggtgccga cgacgctgct gtagacgccg ctgggctgga
     7381 cgccgttgac atcggtccgc agatcctgga agtagccgaa gagggtggac aggaacggcg
     7441 ggctgtc
//
LOCUS       DASUYC010001199         2311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_2072923, whole genome shotgun sequence.
ACCESSION   DASUYC010001199 DASUYC010000000
VERSION     DASUYC010001199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2072923"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="VF276_19630"
     CDS             complement(<1..526)
                     /locus_tag="VF276_19630"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX6609121.1"
                     /translation="MLDPFKVTRLWFSPNLAGIVRSIAFSHDGKIIAGGAGFPPTVYL
                     LDAETGAEVAGLTGHEGQVNSVAFSLDGRMLASGSADGTIKLWDITGSSAARTLEGHT
                     ADVNGVAFNPNGETVASASADGTVRLWHLNTGAELWVSGAGEASVNAVAFSPDGLTLA
                     TGSDDATVRLWDVAR"
     gene            complement(602..>2311)
                     /locus_tag="VF276_19635"
     CDS             complement(602..>2311)
                     /locus_tag="VF276_19635"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEX6609122.1"
                     /translation="GYSDTGIFVSRSTDGGLTWRDPVVAVGEAGGYSTDKQWFAVDNN
                     PASPYYHRMYLTWTEFGACNGCIHMVTSDDGGLTWSDRARQISTNSGNQQFSMPTVLW
                     NGNLFVNWANGNSIVYRISTDGGATFGPESTAGTMNNNLGMPGRAWRINPIPAAAADR
                     ATPAHIVIVWNDGRNNAANGVDIYAVRSADGGATWTPPARLNDDPPNVVKQQAEPWVT
                     TSPNGTFHAIWYDERDDNTPNIVFNIYYTRSDDGGATWIPDIRASDATTDLNIGIPQG
                     PGWNQAAGDYINVVATDSEVYGAWTDTRSGTNEDIYTFHGHLVVTTPTPTSTVTATPG
                     PVTATPGPVTPTPTPSVTATPCAIRFTDVSTEYYAEAVYYLACRGIVSGYSNGDGTMS
                     YRPNNNTTRAQMVKIVVGAFGVPVQTPATPTFADVPPAHPFYTYIESAAAAGIVSGYT
                     CGGSGEPCDATHRPYFRPSADVTRGQLTKLVTIAAGWAVRNPPAATFADVPPGAPFYA
                     FVETAVCHGVISGYTCGSPGEPCSGGPLYFRPGNPATRGQLAKIVYLSLTSGASCATG
                     PDQ"
BASE COUNT          364 a          716 c          800 g          431 t
ORIGIN      
        1 cgcgtgccac atcccacaac ctgacggtcg cgtcgtcgct ccccgttgcc aatgtgagtc
       61 catctgggct aaaggcgaca gcgttaacag atgcctcgcc cgccccacta acccataact
      121 cggcacctgt gttcaggtgc caaagccgga cggtgccatc ggcgctagca cttgctactg
      181 tttccccatt gggattgaag gcgaccccat taacgtctgc cgtatgcccc tctagagtcc
      241 gtgccgcact actacctgtt atgtcccaca gcttaatcgt tccatcggca ctaccacttg
      301 ccagcatacg accgtcaagg ctaaaagcta cggagttaac ctggccttca tgccctgtaa
      361 gccccgcgac ttcggcaccg gtctcggcat ccagcaaata gacagtagga gggaacccag
      421 ccccgcccgc gatgattttc ccatcgtggc tgaaagcaat ggagcggaca atgccggcca
      481 ggtttggaga aaaccaaagc cgagtaacct taaacggatc taacatagga agcattccat
      541 gttggtccag aaaggcactt tatagcaggc tttcccacta tagcgcgccc tcccgaacaa
      601 gctattgatc tggcccggtt gcgcaactgg ccccgcttgt caggctcagg tagacgatct
      661 tcgccaactg gccgcgcgtg gccgggttgc cggggcggaa gtagagcgga ccaccgctgc
      721 acggttcgcc ggggctgccg caggtgtagc cgctgatcac gccgtggcag acggcggttt
      781 ccacgaaggc atagaacggc gcgcccggcg gcacatcggc gaaggtggcg gcgggcgggt
      841 tgcgcacggc ccagcccgcg gcgatcgtca ccagcttggt gagttggccg cgcgtcacgt
      901 cggcggaggg ccggaagtag ggccggtgag tagcgtcgca cggctcgccc gacccgccgc
      961 aggtgtagcc gctgacaatc cccgcggccg ctgccgactc gatgtaggtg tagaacgggt
     1021 gggccggggg cacgtcggcg aaggttggcg tggccggcgt ctgcaccggc acgccgaagg
     1081 cgcccaccac gatcttgacc atctgggcgc gggtggtgtt gttgttcggg cggtagctca
     1141 tggtgccgtc gccgttgctg tagcccgaga cgatgccccg gcaggccagg tagtagacgg
     1201 cctcggcata atactccgtg ctcacgtcgg tgaagcgaat ggcgcacggc gtggcggtga
     1261 cgctgggtgt gggcgtcggc gtgaccggcc ccggcgtggc cgtcaccggc cccggcgtgg
     1321 ccgtcaccgt ggaggtcgga gtcggcgtgg tcacgaccag gtggccgtgg aaggtgtaga
     1381 tgtcctcgtt ggtgccgctg cgcgtgtcgg tccaggcgcc gtagacctcg ctgtcagtgg
     1441 cgaccacgtt gatgtagtcg cccgccgcct ggttccagcc cggcccctgc gggatgccga
     1501 tattcaggtc ggtcgtggcg tcgctggcgc ggatgtcggg aatccaggtc gccccgccgt
     1561 cgtcggagcg ggtgtagtag atgttaaaca cgatattcgg cgtgttgtcg tcgcgctcgt
     1621 cgtaccagat ggcgtggaag gtgccgttgg ggctggtcgt gacccagggc tcggcttgct
     1681 gcttgaccac gttgggcggg tcgtcgttca ggcgggcggg cggcgtccag gtggccccgc
     1741 cgtcggcgga gcgcacggcg taaatgtcca cgccgttggc ggcattgttg cgcccgtcgt
     1801 tccagacgat gacaatatgg gcgggcgtgg cgcggtcggc ggcggccgcc gggatggggt
     1861 tgatgcgcca ggcgcgaccc ggcatcccca ggttgttgtt catggtgccc gccgtgctct
     1921 ccgggccgaa ggtcgcgccg ccgtcggtcg agatgcggta gacgatgctg ttgccgttgg
     1981 cccaattgac gaagaggttg ccgttccaga gcacggtcgg catcgaaaac tgctggttgc
     2041 cgctgttggt cgagatctgg cgggcgcggt cgctccaggt cagcccgccg tcgtctgaag
     2101 tcaccatgtg gatgcagccg ttgcaggcgc cgaactcggt ccaggtcagg tacatgcggt
     2161 ggtagtaggg gctggccggg ttgttgtcca cggcgaacca ctgcttgtcg gtgctgtagc
     2221 cgcccgcttc gcccaccgct accaccgggt cgcgccaggt gagcccgccg tcggtcgagc
     2281 ggctgacgaa gataccggtg tcgctgtagc c
//
LOCUS       DASUYC010001200         2444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_224589,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001200 DASUYC010000000
VERSION     DASUYC010001200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224589"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1580)
                     /locus_tag="VF276_19640"
     CDS             complement(<1..1580)
                     /locus_tag="VF276_19640"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX6609123.1"
                     /translation="MQTTGIKDNSDVSDPEQRPRERLRPLAGSIQDIRTASQDVLRLM
                     GMILDTPVRIVARSEGRGYRIAAIHDTHGAGLQPDQVVGPDDPPAALLRAGEYGQAPD
                     APPDPFLAPLGLACCISVPICLSDGQVAGTLSAADPAPRQFTPAIREALVLLARMLSH
                     EIERTDRERELHILAGISQAFVQQPNLRAVFQELIEQAVSIFEADYCLLHRIDLTTEL
                     VTPVAAHGVALPIGTARRIAPHEESSATIQTARSRRIVWIPDARTDPRPDPRLVARFD
                     LVSILYVPLVVDDVTQGVLTIAHRGRPYQINPALLRVAEILANEAGLALRRHQLWEVM
                     AQRQHELAVLNELSTLCTSQLELEPLLRQIANEIRRVVTGPTDCYIVLLDAQGRLQPA
                     AASGYLHDRYRKLPRARTEPAIARAVIELNAPIRIEDLNQSEYADTQVRRMFPLRGVL
                     GVPMIARGKTLGAILLASARQPRRWTREEVDRLQTAANQVALSIANVQLYEEVATLAV
                     HDPLTGIFNRRYLEQKLR"
     gene            complement(1677..>2444)
                     /locus_tag="VF276_19645"
     CDS             complement(1677..>2444)
                     /locus_tag="VF276_19645"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX6609124.1"
                     /translation="ALSPAGTQRAARREAVALTVAALVYSNGLAILARRRGRIADQAY
                     RWTNPIFLLVLLSTLARARRTSVRTLLREAGVQRAGWPVALGGGLVLGGVLAAPALVF
                     FARPLVLDSPLEYGPIRGLSRRAFWVRVLIELPVGVAVFEELLFRGLLWEAWARAASE
                     PVAWAASSAAFAGWHCAVGVDTMRRTNIGAAATPLPRPLRRHAHTLGVLGGMLSTGVA
                     GMLLGWLRTRAHGNLLGPALAHWIADALLVAALRRGR"
BASE COUNT          385 a          843 c          864 g          352 t
ORIGIN      
        1 tcgcgcagct tctgctccag gtagcggcga ttgaaaatgc cggtcagtgg gtcgtgcacg
       61 gccagggtcg ccacttcctc gtagagttgg acattggcga tggacagcgc cacctggttg
      121 gcggcggttt gcagccggtc cacctcttcg cgggtccagc ggcgcggctg ccgcgcgctg
      181 gcgagcagga tcgcgcccag cgtcttgccc cgcgcgatca tcggcacgcc gagcacgccg
      241 cgcaatggaa acatccggcg cacctgggtg tcggcgtact ccgactggtt cagatcctcg
      301 atgcggatcg gggcgttgag ttcgatcaca gcgcgggcga tggccggttc cgtccgtgcc
      361 cgcggtagtt tgcggtagcg gtcgtgcaga tagccgctgg ccgccgcggg ctgcaagcgg
      421 ccctgggcgt caagcagcac aatatagcaa tcggtcggac ccgtgaccac gcgccggatc
      481 tcgttggcga tctggcgcag cagcggttcc agttcgagtt gcgaggtaca gagggtgctg
      541 agttcgttga gcacggccag ttcatgctgg cgttgcgcca tcacttccca gagctggtgc
      601 cggcgcaggg ccaggcccgc ctcgttggcc aggatctcgg ccacgcgcaa cagcgccggg
      661 ttgatctggt agggccggcc ccgatgggcg atggtcagca caccctgggt cacatcgtcg
      721 accaccagcg gcacatagag gatactcacc aggtcgaagc gcgcgaccag ccgcgggtcg
      781 ggccgtggat cggtgcgcgc gtcgggaatc cagacgatgc ggcggctacg cgcggtctgg
      841 atcgtcgccg acgattcttc gtggggcgcg atgcgccggg cggtgccgat gggcagggct
      901 acgccgtggg cggccaccgg cgtcaccagt tcggtggtga ggtcgatgcg gtgcagcagg
      961 cagtaatcgg cctcgaagat gctgaccgct tgctcgatca gttcctggaa tacggcgcgc
     1021 agattgggct gctggacaaa cgcctggctg atgccggcca ggatgtgtag ctcgcgctcg
     1081 cggtccgtcc gctcgatctc atgactgagc atgcgcgcga gcagcaccag ggcctcgcga
     1141 atcgcgggcg tgaactggcg cggggcggga tcggcggcgc tgagggtgcc ggccacctgg
     1201 ccgtcgctca aacagatggg cacgctgata cagcaggcga gacccagtgg ggccaggaag
     1261 ggatcgggcg gcgcatcggg ggcctgccca tactcgccgg cccgcagcag ggccgccggg
     1321 gggtcgtcgg ggccaacgac ctgatcgggc tgcaacccgg cgccgtgggt gtcgtggatg
     1381 gccgcgatac ggtagccgcg accctcgctg cgcgcgacga tgcgcacagg cgtatcgagg
     1441 atcatgccca tcaagcgcag cacatcctga ctggccgtgc ggatgtcctg gatgctcccc
     1501 gccaggggac gcagccgttc gcgcggtcgc tgctccgggt cgctcacgtc gctattgtcc
     1561 ttgatgccgg tggtctgcac cgctgtccgt agtatctcgt cgccccgccc gaatccggcc
     1621 gccgggcccg ccggtgttga agatgcatta taccatagcc cgccaggccg gatgggttag
     1681 cgcccgcggc gcaacgccgc cacgagcagc gcatcggcga tccaatgcgc gagggcgggg
     1741 ccaagtagat tgccgtgcgc acgggtgcgc aaccatccca gcaacatccc ggccacgccg
     1801 gtcgagagca tgccgccgag cacgccgagg gtatgcgcgt gccgccgcag cgggcggggc
     1861 agcggcgtgg ccgccgcgcc gatgttggtg cgtcgcatgg tatcgacgcc cacggcacaa
     1921 tgccaccccg cgaaggcggc gctactcgcc gcccaggcca ccggctcgga agcggcccgc
     1981 gcccacgcct cccagagcaa cccgcggaac agcaactctt caaacaccgc cacgcccacc
     2041 ggcaactcga tcagcacccg cacccagaac gcccgccgcg agagcccgcg gatggggccg
     2101 tactccagcg gcgagtcgag caccaggggc cgggcaaaaa agaccagcgc gggggcggcc
     2161 agcacgcccc ccagcaccag gccgccgccc agcgccaccg gccaccccgc gcgctgcacc
     2221 ccggcctcgc gcagcagggt acgcacggaa gtccgccgcg cccgcgccag cgtgctcagc
     2281 aggacgagca gaaagatagg gttcgtccaa cgataggcct ggtcggcgat gcgaccccgc
     2341 cgcctcgcca ggattgccag cccgttgctg tagaccaggg ccgccaccgt cagggccacc
     2401 gcttctcgcc gtgccgcgcg ttgcgtgccc gctgggctca atgc
//
LOCUS       DASUYC010001201         2742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1241392, whole genome shotgun sequence.
ACCESSION   DASUYC010001201 DASUYC010000000
VERSION     DASUYC010001201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241392"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VF276_19650"
     CDS             <1..218
                     /locus_tag="VF276_19650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6609125.1"
                     /translation="ANVTIILGEETWGPYRSPQLGIVFLANLPWILCPLYLIYRMRTA
                     PHPFTEVISAGATQATLPGLHPHEMDG"
     gene            386..1432
                     /locus_tag="VF276_19655"
     CDS             386..1432
                     /locus_tag="VF276_19655"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015909272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEX6609126.1"
                     /translation="MDEHALFTAPHRRRNPLTGEWLLVSPQRTARPWQGQVETPPPET
                     RPAYDPTCYLCPGNERAGGARNPDYTGTFVFENDFAALRPTTPPLDLARGMDGKPPLL
                     HAESERGICRVVCFSPRHDLTLAGMDLPGIERVLATWTAQYTELGSLPWVRHVQIFEN
                     RGAMMGASNPHPHGQIWANERLPNEPAKELHEQRAYHATGGGCLLCDYLALELREGTR
                     LVCRNDHFVALVPFWAVWPFETLVLPTAHHGALPDLDPAAATGLADILRRVTRRYDNL
                     FGVSFPYSMGFHQRPTDGAAYPEGHLHAHFYPPLLRSATVRKFMVGYEMLGQPQRDIT
                     PESAAERLRGLSEE"
     gene            1967..>2742
                     /locus_tag="VF276_19660"
     CDS             1967..>2742
                     /locus_tag="VF276_19660"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6609127.1"
                     /translation="MQPTILVPLDGSARAEAILPAAVAMAQATHSSLSLLQVVVSEST
                     TGIHGASDRQWVDARTPACAYLESVAARVLQAGVPVDVEVLVGHATTRIVQATRQDPR
                     ISLIAMATHGRGGLPRAVFGSIAEEVLRSVAVPVLLVRDGQAAVPPLTAGCTILVPLD
                     GSAFARQAVGTAQMLGRAFGATLLLAAVVPPREPADPPQTAPASSWRNEAQHRETGRL
                     MFELDKTARQLEAAGAPVRRQLLHGKPGEALVAQAGTAATA"
BASE COUNT          433 a         1004 c          917 g          388 t
ORIGIN      
        1 tggccaacgt caccatcatc ctcggcgaag agacctgggg gccataccgc tcgccgcaac
       61 tcggcatcgt gttcctggcg aacttgccct ggatcctctg cccgctgtat ctgatctacc
      121 gcatgcggac cgcgccacac cccttcactg aggtgatctc cgcaggcgca acccaggcga
      181 ccctgccggg gctgcatcct cacgagatgg acggctaagc cggggggagc ggtttacatg
      241 gacaccgccc cggcgcccat gccggcccgc gcgcatcact ccggcgaaga cgcgtcgttg
      301 atcattcaga caatggaaat ggggcaaccg tgcgtcattt tccgcgatgg cgccaaccac
      361 ggaccgatca gcgagggcag acttgatgga cgagcacgcg cttttcaccg cgccgcaccg
      421 ccggcgcaat ccgctcaccg gcgagtggct gctggtctcc ccgcagcgca ccgcgcgccc
      481 gtggcagggg caggtcgaga ccccgccgcc ggaaacgcgg cccgcgtacg atcccacctg
      541 ctacctgtgc cccggcaacg agcgcgcggg gggcgcacgc aatcccgact acaccggcac
      601 cttcgtcttc gagaacgact tcgcggccct gcgtcccacc accccgcccc tggacctggc
      661 ccgcggcatg gacggcaagc cgcccctcct gcacgccgag agcgagcgcg gcatctgccg
      721 ggtggtctgt ttctcgccgc gccacgatct cactctggcc ggcatggatt tgccgggcat
      781 cgagcgcgtg ctggcaacct ggaccgcgca gtataccgaa ctgggcagcc tgccctgggt
      841 gcgccatgtt cagatcttcg agaaccgcgg ggcgatgatg ggcgcgagca atccccaccc
      901 gcacggccag atctgggcca acgagcggct gcccaacgag ccggccaagg aactgcacga
      961 gcagcgcgcc taccacgcga cgggcggcgg ctgcttgctc tgcgactacc tggccctgga
     1021 actccgcgaa ggcacgcgcc tggtgtgccg caacgaccac ttcgtggccc tggtgccctt
     1081 ctgggccgtc tggcccttcg agacgctggt gttgcccacg gcgcaccacg gcgcgctgcc
     1141 tgacctcgac ccggcggccg ccacggggct ggccgatatc ctgcgccggg tgacgcggcg
     1201 ctatgacaac ctgttcgggg tcagcttccc ctacagcatg gggttccacc agcgcccgac
     1261 ggacggcgcc gcctaccccg aggggcacct gcacgcccac ttctacccgc ccctgctgcg
     1321 ctcggccacc gtccgcaaat tcatggtggg ctacgagatg ctggggcaac cgcagcgcga
     1381 catcacgccg gagagcgccg ccgaacggct gcgcggcctg tccgaggagt agcgggcagc
     1441 cccctcggcg gcaggtgggc cgggccggcc gcagggggcg ctaaagaccg gcaaggcgcc
     1501 aggatcatcc gccacaagcg cggcccgctg ccgcatcggc ggctccccgg cggaggcgcc
     1561 ggccactcgt accaccgaca cggcccctgg cctgcgccgg cctaccccgc acgaagtgtt
     1621 acgcaattgc cacctggtgt atacccggtt cgtcaccctg gcgcgctaca ctggcgatag
     1681 cgagacggca gaagtgaccg cgcaggagcg cgctgcctga cccctctcag gccaggagtg
     1741 agaggaatgc tcgctgcaca gaagttcccc gatgggaagc cgattctctg acggcaagcc
     1801 gcggagcgcg gcaggcctcg ccacaccgcc cggcgataag ccgcccggcg cgccccgctc
     1861 agcatccagg cgcggcccgc gacagcgaca gcaggcgggt ggaccgggat ctttcgccgc
     1921 gtgtcgtgat cgggtggcgg cgcgcaccag gaagggagat gcagcaatgc aaccaacgat
     1981 tctagtgccg ctggatggat cggcgcgggc cgaagcgatc ttgcctgctg cggtggctat
     2041 ggcgcaggcg acgcatagtt ccttgagcct gctgcaggtg gtagtctccg agagcaccac
     2101 cggcatccat ggcgcgtcgg atcggcaatg ggtcgacgcg cggacgccgg cctgcgccta
     2161 cttagagagc gttgcagcgc gcgtgcttca ggcgggggtg ccggtggacg ttgaagtgct
     2221 ggtcggccac gccaccaccc gtatcgtgca agcgacccgc caggatccgc gcatcagcct
     2281 catcgccatg gcaacgcatg ggcgcggcgg cttgccccgc gccgtgtttg gcagcatcgc
     2341 cgaagaagtc ttgcgcagcg tggccgtgcc cgtgctgctc gtgcgtgacg ggcaagcggc
     2401 ggtgccgccc ctgacagccg gttgtaccat cctggtgccg ctcgacggct ccgcgttcgc
     2461 ccgccaggcg gtgggcacgg cccagatgct gggccgcgcc tttggggcga ccttgctgct
     2521 ggcggcggtc gtccctcccc gcgaaccggc ggacccgccc cagacggcgc cagcctcatc
     2581 ctggcgcaac gaagcccagc accgcgagac cggtcggctc atgttcgagt tagacaagac
     2641 cgcacggcaa ctggaggccg ccggagcacc cgtgcggcgc cagttgctac acggcaagcc
     2701 gggggaagcg cttgtcgccc aggccggcac ggccgccacg gc
//
LOCUS       DASUYC010001202         2635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885 k141_688228,
            whole genome shotgun sequence.
ACCESSION   DASUYC010001202 DASUYC010000000
VERSION     DASUYC010001202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688228"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VF276_19665"
     CDS             complement(<1..507)
                     /locus_tag="VF276_19665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015622785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6609128.1"
                     /translation="MAVKFGVFVPQGWKMDLVGIPDPSEQYEAMARAARAADEAGGYD
                     SIWVYDHFHTVPEPTLETTFEAWTITAGLVRDTQRVRVGQMVTCNGYRNPALLAKMAS
                     TVDAMAHGRLICGLGAGWYEHEWRAYGYGFPDTPVRMGMFREACQIVYSMWTEDKPTF
                     SGKYYTIDG"
     gene            complement(774..1487)
                     /locus_tag="VF276_19670"
     CDS             complement(774..1487)
                     /locus_tag="VF276_19670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6609129.1"
                     /translation="MRTEQLPDGAPALFRLNGVPVQVPPAYWGDVIALGGGLVWLAGW
                     RRPERSWGGRLVVGGLALALTVLADLVHVFGHTVSARLAGAPMDRVRVALGIPRTLYD
                     NNDVPPATHRLRALGGPIANLLALLLSLLWRRATRPASAVRDLADVSCLGHGVLFFGS
                     LVPLPIFDAGTILKWTLVEQGRTEAEADALVHQVSELIGLGALTTGVVVVLQGRRAVG
                     GALIAGGAVAIAAGRDLIR"
     gene            1853..>2635
                     /locus_tag="VF276_19675"
     CDS             1853..>2635
                     /locus_tag="VF276_19675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEX6609130.1"
                     /translation="MHHDIYMTAKKIARRLALIAPLAYRRSAPLPPLLFKQLAGPDAP
                     PPLGPQQDDAGWEVVLPNTYWGGHNCNFALRGEFTVPPDFDPASPVALFLPLGDSGSF
                     AHPEALAYIDGAAYATTDYFHQEFRLPDACRDGRPHALLLQGWTGKVSDSVAVDNGQR
                     LFMHQCAVVQIDQPTRDFLAVARTALQTANVLDDLEPAKTRLLNALDAAFKILDTREP
                     FGDSFYASVPAARETLDAGIAQAGPPLDAEIIAAGHAHIDVAW"
BASE COUNT          455 a          961 c          828 g          391 t
ORIGIN      
        1 cccgtcgatc gtgtaatatt tcccgctaaa cgtcggcttg tcctcggtcc acatgctgta
       61 aacgatctgg catgcttcgc ggaacatgcc catgcgcacc ggggtgtcgg ggaagccata
      121 gccgtaggcg cgccactcgt gctcgtacca gcccgcgccc agcccgcaga tcagccgccc
      181 gtgggccatg gcgtccaccg tcgaggccat cttcgccagc agggccgggt tgcggtagcc
      241 gttgcacgtc accatctggc ccacgcgcac acgctgcgta tcgcggacca gccccgccgt
      301 gatcgtccac gcctcgaacg tcgtctccag cgtcggctca ggcaccgtgt gaaagtggtc
      361 gtagacccag atcgaatcgt acccgccggc ctcgtcggcg gcccgcgcgg cacgagccat
      421 cgcctcgtac tgctcgctgg ggtcggggat gcccaccagg tccatcttcc agccctgcgg
      481 cacaaacacg ccaaacttta ccgccatgtt gttgctcctt tcaccttctg cgcgtgcggc
      541 atcgccacat cgataaccgg gacaggcgtt gcaaaaagca tgcccgatgc cccctgacag
      601 cgaacacgtt cgagaatggt tggcacgcct accagcagag caagcggtaa tccccttatc
      661 cggctaatcc cgcacctccg gccgatcgcg gatgaccata agcgtcgcca gcgttgctgc
      721 ggggccgggc agacgtaagt ctgcccgcca gaaaggggtg cttcgataaa tccttagcgg
      781 atgagatcgc gccccgcggc gatggcgacg gcgccgccgg caatcagtgc gccgcccacg
      841 gctcgacggc cctggagcac caccacgacg cccgtggtga gcgcgcccag gccgattagc
      901 tcactcacct ggtggaccag ggcatcggcc tcggcttccg tccggccctg ctcgacgagg
      961 gtccacttga ggatggtgcc ggcgtcgaag atcggcaggg gcacgaggct gccgaagaag
     1021 aggaccccgt ggccgaggca ggacacgtca gccaggtcgc ggaccgccga ggcgggccgc
     1081 gtcgcccggc gccagagcag gctgagcagc agggcgagca ggttggcaat tggcccgccg
     1141 agggcgcgca ggcggtgggt ggcgggcggc acgtcgttgt tgtcgtagag cgtgcgcgga
     1201 atacccagcg cgacccgcac gcggtccatc ggcgccccgg ccaggcgcgc gctgacggtg
     1261 tgcccgaaga catgcaccag gtcggcgagc acggtgaggg ccagggccag cccgccgacc
     1321 acgagacgcc cgccccaact ccgctccggg cggcgccacc cggccagcca gaccagcccg
     1381 ccccccagcg cgatgacgtc gccccagtag gcgggcggca cttggaccgg cacgccgttg
     1441 agccggaaga gagccggcgc tccgtcaggt aattgctcgg tacgcataac gatcaggccc
     1501 cgtggtgctc ggcgcgggcg cgatggaccc gacaccagca cgcattataa aaagcggcca
     1561 cctaccccac cgggctgccg agagcccggc aggcatgcgg cgtcggcaga tttctcaggt
     1621 aaccactagt ggaaaggtat atcagcagtc ctggaaaggg ctggtccatg ccctgtctcc
     1681 ttcgtagact accataaggc gacaggtttg taaagcacgt ggtgggcgac ggcagcagaa
     1741 cctccagtcg cacggatatg tatatcacta ctgcccttga caggtccgaa cccgcccgcc
     1801 atactcatag tatcgatcga tcaccccgtt tcttgcccaa gggaggctcc atttgcatca
     1861 cgatatttat atgacggcca agaagatcgc ccgccgcctg gccctgatcg cgccgctggc
     1921 ctaccgccgc agcgcgccgc tgccgccgct gctgttcaag caactggccg gccccgacgc
     1981 gccgccgccg ctcggtccgc agcaggacga cgcgggctgg gaggtggtgc tgcccaatac
     2041 ctactggggc ggacacaatt gcaacttcgc cctgcgcggc gagttcaccg tcccgcccga
     2101 cttcgacccg gccagcccgg tcgcgctgtt cctgccgctc ggcgactccg gctcgttcgc
     2161 ccaccccgaa gccctggcct acatcgacgg cgccgcctac gccaccacgg actactttca
     2221 ccaggagttc cggctgcccg acgcctgccg cgacgggcgg ccccacgccc tgctgctcca
     2281 gggctggacc ggcaaggtga gcgactcggt ggccgtagac aacgggcagc gcctgttcat
     2341 gcaccagtgt gccgtcgtgc agatcgacca gcccacgcgc gacttcctgg ccgtggcgcg
     2401 caccgcgctg caaaccgcca acgtgctcga cgacctcgaa ccggccaaaa cccgcctgct
     2461 caacgcgctc gacgccgcgt tcaagatcct cgacacgcgc gagccgttcg gcgacagctt
     2521 ctatgccagc gtgcccgccg cccgcgaaac gctggacgcc ggcatcgccc aggccgggcc
     2581 gccgctcgac gccgagatta tcgccgccgg gcacgcccac atcgacgtgg cctgg
//
LOCUS       DASUYC010001203         2267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Chloroflexia bacterium isolate SMAG_U1885
            k141_1842646, whole genome shotgun sequence.
ACCESSION   DASUYC010001203 DASUYC010000000
VERSION     DASUYC010001203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961216
            Sequence Read Archive: SRR25158381
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chloroflexia bacterium (soil metagenome)
  ORGANISM  Chloroflexia bacterium
            Bacteria; Chloroflexota; Chloroflexia.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:29:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,938
            CDSs (total)                      :: 3,897
            Genes (coding)                    :: 3,862
            CDSs (with protein)               :: 3,862
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 2 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Chloroflexia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1842646"
                     /isolate="SMAG_U1885"
                     /isolation_source="soil"
                     /db_xref="taxon:2448782"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.77305556 N 100.6725 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VF276_19680"
     CDS             <1..330
                     /locus_tag="VF276_19680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX6609131.1"
                     /translation="GICISLDDFGTGFSNMSYLQQLPIDALKIDRSFVHDVDRNERNA
                     SICRAMIALGHGLGLQLVAEGVETPEQHAWLQRHGCDRAQGFGLGRPAPLQEVLATLG
                     GRDLGAA"
     gene            complement(340..1506)
                     /locus_tag="VF276_19685"
     CDS             complement(340..1506)
                     /locus_tag="VF276_19685"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEX6609132.1"
                     /translation="MIEEAAIQGALLAAAATWLLIWLLKPAALRLNLLDHPRGRKAHE
                     APTPVTGGLAMTLGVALGGFAAWSSLSPPRQALYLGMTLLLAVGLLDDLYDIRWWWRI
                     LAQVAAALILYYVGGVRVAQIGDALGFPHHTLGPLSLPLTVFATVGVINAITLIDGVD
                     GLAGTLVLCALAMFCCAALYAGNASLAGRVLLLGGAVAGFLAWNFRVPGRARAAAFMG
                     NSGSALLGLAIAWVCFRLTQNQGHPVSPVLALWLLPVPVMDCLVLIVRRIREGRSPFS
                     AGRDHIHHYLSEAGFGPMGICAVLGGFSLLVGLAAGQAMRLDIPNVLILAAFLGLCVA
                     WYALSARRRHAVRLFARLRAGLLGDLRGATVLLVPEASNDDVAVVPPRARTGSE"
     gene            complement(1654..>2267)
                     /locus_tag="VF276_19690"
     CDS             complement(1654..>2267)
                     /locus_tag="VF276_19690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007817380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6609133.1"
                     /translation="RAARRARHPVADMDHCGIHRQVADLHLASIGQGFLSTLGPAFLT
                     LLYESMDTADHSVLLVEIRDDCVAGFVAGGHGMGPIYRRMLGKPFRLIRALFPSLFRP
                     ERLLRILEILRYGRATDPALPKAELLSIAVAPAYRGQGIADALYRRLVGYFNSQGTDA
                     FKITVGEHLGPAHRFYLRMGAEPVSSVEVHRGEGSVVYIHRLG"
BASE COUNT          413 a          774 c          773 g          307 t
ORIGIN      
        1 ggcatctgca tctcgctgga cgacttcggc accggcttct cgaacatgtc gtacctgcag
       61 cagctgccga tcgacgcgct gaagatcgac cgttccttcg tccacgacgt cgaccgcaac
      121 gaacgcaacg cctcgatctg ccgcgcgatg atcgcgctcg gccatgggct cggcctgcag
      181 ctggtggccg aaggcgtcga gaccccggaa cagcacgcct ggctgcagcg ccacggctgc
      241 gaccgggcgc aaggcttcgg actcgggcgc cccgcgccgc tgcaggaagt gctggcgacg
      301 ctgggcggac gcgacctggg ggccgcgtag accgccggcc tactcggaac cggtgcgcgc
      361 ccgcggcggc accacggcga cgtcgtcgtt gctcgcttcc ggcaccagca gcaccgtcgc
      421 cccgcgcagg tcgcccagca acccggcgcg cagccgcgcg aacagccgca ccgcatgccg
      481 gcgccgcgcc gacagcgcgt accacgcgac gcacaggccg aggaacgcgg cgaggatcag
      541 cacgttcggg atgtccaggc gcatggcctg ccccgccgcc agcccgacca gcaggctgaa
      601 gccgccgagc accgcgcaga tgcccatcgg accgaaaccg gcctcgctga ggtaatggtg
      661 gatgtggtcg cgtccggcgg agaacggcga gcgcccctcc cggatccggc ggacgatcag
      721 gaccaggcag tccatcaccg ggaccggcag cagccacagc gccagcaccg ggctcacggg
      781 gtggccctgg ttctgggtga ggcggaagca gacccaggcg atcgccaggc ccagcagtgc
      841 gctgccggag ttgcccatga aagccgcggc gcgggccctt ccggggaccc ggaagttcca
      901 ggccaggaag ccggcgaccg cgccgccgag cagcagcacc cgccccgcca gcgaggcatt
      961 gcccgcgtac agcgccgcgc agcagaacat cgccagcgcg caaagcacca gcgttccggc
     1021 caggccgtcc accccgtcga tcagggtgat cgcgttgatc accccgaccg tggcgaagac
     1081 cgtcagcggc agggacagcg ggccgagcgt gtggtggggg aagcccagcg catcgccgat
     1141 ctgcgccacc cggacgccgc cgacgtagta gaggatcagg gcggcggcga cctgggccag
     1201 gatgcgccac caccaacgga tatcgtagag gtcgtcgagc aggccgacgg cgagcagcag
     1261 ggtcatgccc aggtacaggg cctgccgggg cggcgacagg ctggaccagg ccgcgaaccc
     1321 gcccagcgcc acgcccagcg tcatggccag gccgccggtg accggcgtcg gagcctcgtg
     1381 ggccttgcgg ccgcgggggt ggtcgagcag gttcaggcgc agggcggcgg gcttgaggag
     1441 ccagatcagg agccaggtcg ccgcggcggc cagcagggca ccctggattg cggcttcctc
     1501 gatcatcctg aatgcctgcc ttcttctgtg ggcgggtcgc gtgcgcgccg atcgcgcggc
     1561 ggacggggga tagccgctcg atcttagttt gtgaaatgtg acggaattgg cacaaaaagc
     1621 cggacagcca tcgcttgttc acggaaggca cagctagcca aggcgatgga tatagacgac
     1681 cgatccttcg cctcgatgca cctcgacgct ggaaacaggc tcggccccca tccgtagata
     1741 gaaccggtgt gcaggcccca gatgctcgcc cacggtgatc ttgaaggcat ccgttccttg
     1801 tgagttgaaa tatccgacga gtcttcgata cagcgcgtcg gcaataccct gcccacgata
     1861 cgccggagct acggcgatac tgagcagttc cgccttgggc aacgcggggt cagttgctcg
     1921 accgtagcgg agtatctcca ggatccgcag aagacgctcc gggcggaata gcgacggaaa
     1981 caacgcccgg atcaatctga agggctttcc gagcatgcgt cgatagatag gccccatgcc
     2041 gtgcccacct gcgacgaagc cggcgacgca atcatcgcgt atttccacca gcaatacgct
     2101 gtgatcggcc gtgtccatgg attcgtacag cagcgtcagg aatgcagggc ctaacgtcga
     2161 caggaatccc tgccctatgc tggcgagatg caagtcggcc acctgtcgat ggatgccgca
     2221 atgatccatg tcggctaccg gatggcgagc ccttctggcg gctcggg
//
